LOCUS       DASRUM010000001        22407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1505556, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000001 DASRUM010000000
VERSION     DASRUM010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 22407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22407
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505556"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1615
                     /locus_tag="VFF22_00005"
     CDS             56..1615
                     /locus_tag="VFF22_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HZX15237.1"
                     /translation="MSKIISAAAAVARIPDNANLATGGFVGIGFAEEIAIALEQRFLT
                     EQAPLGLTLVYAAGQGDGKGRGLNHLAHEGLVRRVIGGHWGLVPGLQKLAVDNRIEAY
                     NLPQGVISQLFRDIAAGKPGQLSRVGLGTYVDPRHGGGKLNACTTTELVRLMPIDGED
                     YLFYPTFPIHVAVVRATSSDADGNLSFEREALTIESLAIAMAARNSGGLVIAQVERIV
                     ERGSLNARDVKIPGILVDCVVLAEPANHQQTFATAYNPAFAGETRVPMNSLKAMPLDV
                     RKLIARRAALELQAGAVVNLGIGMPEGVAAVAAEEGVIERLTLTAEPGVIGGIPASGL
                     DFGAASNHSALLDQPYQFDFYDGGGLDIAFLGLAQADAAGNLNVSKFGSRLAGAGGFI
                     NISQNAKQVVFLGTFSAGPQDIRIEDGQLRIIKDGELRKFVVEVEHRTFAGHQAAERR
                     QPVLYVTERCVLRLTLDGLELIEVAPGVDIQRDILDRMDFAPIVREPKLMDARLFLPA
                     CIGLSERLTAN"
     gene            1824..2378
                     /locus_tag="VFF22_00010"
     CDS             1824..2378
                     /locus_tag="VFF22_00010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HZX15238.1"
                     /translation="MNKTYSVAVLVGSLRKASINRKLALALTELAPPSLQLKIIEIGE
                     LPLYNEDIDQDNPPAAYKDFRSQLQAADALLFVTPEYNRSVPAPMKNAIDVGSRPYGK
                     SAFSGKPGAVLSASPGAIGGFAANHHLRQSMVFLDVHMMQQPEAYLGGAGSFFDEAGK
                     LDDGIKPFLQKFIDAYAAWVARHL"
     gene            complement(2390..2581)
                     /locus_tag="VFF22_00015"
     CDS             complement(2390..2581)
                     /locus_tag="VFF22_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15239.1"
                     /translation="MTIFQPLTQGLFDPRLQLEAETSEYRAYPGSICLGMPDIHADII
                     QTIAAIDRLAGQLRQPPAQ"
     gene            complement(2777..4690)
                     /locus_tag="VFF22_00020"
     CDS             complement(2777..4690)
                     /locus_tag="VFF22_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020298792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZX15240.1"
                     /translation="MNFFEQQDRARRNSGRLLILMALAVLSLITLTSLALGAIWQLFG
                     NDQNSLGLLDGPGSLLPSWPLVGSVALAVIGVVLLGSLYKQLQLSRGGQAIAERLGGR
                     LINLSPQNLEERRLLNVVEEMALASGTSVPPVYVLDDAGINAFAAGLTPQDAVIGITR
                     GAICQLSRDELQGVIAHEFSHIFHGDMRLNTRLVSVLHGILLLGLIGGFLLRSSGRGS
                     RSSRDNSVAIVLAIGVTLWVLGYAGTFFGNLIKAAVSRQREFLADASAVQYTRNPDSI
                     AGALKKIGGNLYGSQLQAGHAAEFSHMYFAAGVRQAVDGMLATHPPLDQRIRRITPGW
                     DGRYPHVAELGAATPAASDSAVNLHDLSAFVPGLSAASASEAIGAIGDPQAPHLLAAR
                     STLAHLDIALSEAAHSCSGAQALLYGLLLDPVEKQAAQQLQLLQPRIAAEIAAQLELM
                     REPLRQLAPGMRLPLLDLAMPALKQLAADQFAALKANLALLIRADGRVALLEWTLLRI
                     VERNVEGVRAQTSRYQLQELAAETRVVLSLLARAGGVDEPAAQLAFKHAGSELPFQLD
                     QILPVSASGLVALEPALRRLSLLWPLQKPQLLKAMARCIEHDGRINAEEAELMRAVAD
                     ILDCPMPPLLSQG"
     gene            complement(4708..5304)
                     /locus_tag="VFF22_00025"
     CDS             complement(4708..5304)
                     /locus_tag="VFF22_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009043301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZX15241.1"
                     /translation="MSISTMIFLAVVVALIFYVISIYNQLVALRNRFQNAFAQIEVQL
                     KRRYDLIPNLVETAKGYMAHERETLEAVIAARNNAVTGLKAAAAQPGNAQSMAQLGGA
                     EGLLNSALGRLNVTLEAYPDLKASANMQQLSEELSSTENKVAFARQAFNDAVMAYNTY
                     KQAFPPVLLAASFGHAQDASLLEFADSVAIQEAPKVSF"
     gene            5499..6089
                     /locus_tag="VFF22_00030"
     CDS             5499..6089
                     /locus_tag="VFF22_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZX15242.1"
                     /translation="MKKISTRDKLIHAMADALQRKGLHGVGLSELLEIAGAPKGSLYH
                     HFPGGKSELAVAAIDHISRHIDSLFSQLFAQQTDPLKALHNWLQGALQQLENSQFERG
                     CPLATIALESGPEDVEIRAALQRSFAAVRQALSQQLHAYGYPSEQADNLAALFVALYE
                     GGLLQARVAGSSEPLKRAATALFNLTREQFPGGVRP"
     gene            6086..6970
                     /locus_tag="VFF22_00035"
     CDS             6086..6970
                     /locus_tag="VFF22_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZX15243.1"
                     /translation="MSLNTPAILSESVQLPALDGYPLTATLYHAPEPIAQLVIAGATG
                     VPQGFYRRFAEYAAQRGYSTLTLDYRGIGQSKPASLRGFAMDYLDWAHLDLAAAVDQH
                     RHPQRPLYMVGHSFGGHAFGLLPNHDQVQGFYTFGTGAGWHGWMPKAEQLRVLTMWRL
                     IGPLLTRYKGYLAWSKLGMGEDLPLGVYKQWKRWCSYPRYFFEDPQMPELAERFAQVS
                     TPIIAANALDDLWAPPASRDAFMAAYSAAPYQARTIDSAASIGAIGHMGYFRPAAQSL
                     WDETLNWFAELQAQQQAA"
     gene            6999..7406
                     /locus_tag="VFF22_00040"
     CDS             6999..7406
                     /locus_tag="VFF22_00040"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003179505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZX15244.1"
                     /translation="MSMIPAGFTPLFRSSPFLDLLGPIYNQRSDTGLVIGLRAEEKHC
                     NARGVVHGGVLSSLADVALGYNCAFAQEPPTPIVTSSLTIDYAGTAKLGDWITIETDV
                     QKVGKSLAFANCYFVVESKRIARASAVFSVVTA"
     gene            7802..8458
                     /locus_tag="VFF22_00045"
     CDS             7802..8458
                     /locus_tag="VFF22_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HZX15245.1"
                     /translation="MRSFLFFIGCLAATISSLASANGQNQLSNPKAATQLFWNELYGQ
                     GGSTLYCGKAFTEESGQLKASPIYSSKQLKSALRCITNRQCGIMNPRYVFMAADLHNL
                     YPAMTRVELVRRNAQFGELDDSVPSKFADIGCDMKTSFQLVEPRDEAKGNVARAIFYM
                     HVEYDLPIVGQVQMYKKWHQMDPPDAEEKARNDKIASLQGTRNRFIDNPELVEQQINN
                     "
     gene            complement(8465..9178)
                     /gene="hutC"
                     /locus_tag="VFF22_00050"
     CDS             complement(8465..9178)
                     /gene="hutC"
                     /locus_tag="VFF22_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine utilization repressor"
                     /protein_id="HZX15246.1"
                     /translation="MSSTTPRYKAIEAFLLERIHSGAYPVNHQIPAEEQLARDFGVSR
                     MTANKAIRDLVQKGYLLRQAGLGTFVTDRKAESSLHEVLNIATEVRGRGHAYSNDIIR
                     CEALAADDEVALRLGLRLGAEVFHSILVHREDGVPIQLEDRFVNPRWVPHYLDSDFSR
                     QTPNEVLVSSCPISDVEHVVEAVLVDQQVADWLAIEVASPCLSVIRRTWSDEHLISYA
                     RLIHPGDRYKLRSSHKPRR"
     gene            complement(9318..10853)
                     /gene="hutH"
                     /locus_tag="VFF22_00055"
     CDS             complement(9318..10853)
                     /gene="hutH"
                     /locus_tag="VFF22_00055"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HZX15247.1"
                     /translation="MSLFTLHPGQLTLAQLRAAYQAPLRVSLAASAYPAIEASVACVN
                     GIIAEGRTAYGINTGFGLLASTRIANDDLEKLQRSLVLSHAAGIGAPLDDAMVRLIML
                     LKVNSLARGFSGIRRQVIEALIALINAEVYPHIPLKGSVGASGDLAPLAHMSLVLLGE
                     SQTRYRGEWLPATEALAVAGLQPMTLAAKEGLALLNGTQVSTAYALRGLFEAEDLFAA
                     ASVCGGLTVEAALGSRAPFDPRIHAARGQRGQIDAAAIYRHLLGDSSEVGRSHEACDK
                     VQDPYSLRCQPQVMGACLTQLRQAAEVLAVEANAVSDNPLVFAAEGEVISGGNFHAEP
                     VAMAADNIALAIAEIGALSERRTSLMMDKHMSQLPPFLVANGGVNSGFMIAQVTAAAL
                     ASENKALAHPHSVDSLPTSANQEDHVSMAPAAGKRLWEMAANTRGVLAIEWLGACQGL
                     DFREGLKSSPLLEQARQALRAQVPYYVEDRFFAPDIEAANALLAERVLSPLLPSGVLP
                     SLA"
     gene            complement(10850..12532)
                     /gene="hutU"
                     /locus_tag="VFF22_00060"
     CDS             complement(10850..12532)
                     /gene="hutU"
                     /locus_tag="VFF22_00060"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HZX15248.1"
                     /translation="MSLFRDTEIRAARGTTLTAKSWLTEAPLRMLMNNLDPEVAENPK
                     ELLVYGGIGRAARNWECYDKIVEVLTRLEDNQTLLIQSGKPVGVFETHKDAPRVLLAN
                     SNLVPHWATWEHFNELDAKGLAMYGQMTAGSWIYIGSQGIVQGTYETFVEAGRQHYGG
                     NLKGRWVLTAGLGGMGGAQPLAATLAGACSLNIECQQSRIDFRLKTRYVDEQATDLDD
                     ALARIDTYCSEGKAVSIALCGNAAEILPELVKRGVRPDMVTDQTSAHDPRHGYLPAGW
                     SWDEYVARGKTEEALVVKAAKQSMAVHVRAMLAFQQMGVPTFDYGNNIRQMALQEGVD
                     NAFDFSGFVPAYIRPLFCQGIGPFRWVALSGDPEDIYKTDAKVKELIPDDAHLHNWLD
                     MARERISFQGLPARICWLGLKDRARLAQAFNDMVASGELKAPIVIGRDHLDSGSVASP
                     NRETEAMQDGSDAVSDWPLLNALLNTAGGATWVSLHHGGGVGMGYSQHSGVVIVADGT
                     PEARARLGRVLRNDPGTGVMRHADAGYEIAIDCAREQGLDLPMISATQGAHA"
     gene            complement(12576..13532)
                     /gene="hutG"
                     /locus_tag="VFF22_00065"
     CDS             complement(12576..13532)
                     /gene="hutG"
                     /locus_tag="VFF22_00065"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050415 - formimidoylglutamase activity
                     [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HZX15249.1"
                     /translation="MHADRNSMDLWQGRVDPEADSSRWHQRIQELSADSQPGVALLGF
                     ASDEGVRRNHGRVGAVNGPLAMRKALANLAWHRQGAAYDAGDVLCADGDLESAQARLG
                     HNVCALLDAGHLPIVLGGGHEVAFGSWQGIAEHMAGGSAPRMAAPRIGIINFDAHFDL
                     RDPAHARSSGTPFAQIAEQCAACGWPFAYACLGVSRASNTRALFQRAAELNVLVREDR
                     EIREGSLTALSAELQAFIAGCDAIYLTIDIDVLPACEAPGVSAPAARGVPLALLEPLL
                     ETVRDSGKLRLMDLAELNPDHDIDSRTAKVTARLIHLLTLTP"
     gene            complement(13522..14757)
                     /gene="hutI"
                     /locus_tag="VFF22_00070"
     CDS             complement(13522..14757)
                     /gene="hutI"
                     /locus_tag="VFF22_00070"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HZX15250.1"
                     /translation="MSPSALRLLWRELCLFDGLTTLAEPINLLVADGKVAGLWPTSAF
                     PEAMAEGALEAGRGGVMTPGLVDCHTHLVYAGNRAAEFEQRLEGVSYAEIAKAGGGIL
                     STVRATRAASEDELLAQSLPRLQELLDDGVTTLEIKSGYGLSTADELKMLRVARRLGE
                     LLPLRITTTLLGAHALPPEFADDSDGYIDLVCNEMIPAAVAEGLADAVDVFCENIAFS
                     PAQCELVFQAAQRHGLPIKVHAEQLSNQGASALAARYQGLSADHIEFLDEAGVQAMAA
                     AGTVATLLPGAFYTLRETQLPPIALLRQYKVPMALASDANPGTSPICFPTLTLNLACT
                     LFRLTPREALAGMTAHAARALGRPDLGRIEVGAPADLCLWDVQQPAELAYAVQPGRLR
                     QRIFNGAISYSKEANSYAR"
     gene            complement(15228..17006)
                     /locus_tag="VFF22_00075"
     CDS             complement(15228..17006)
                     /locus_tag="VFF22_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HZX15251.1"
                     /translation="MADYKAPLRDMRFVLNEVFEVSKLWAQLPALAEVVDEDTAAAIL
                     EEAGKVTGGVIAPLNRSGDEEGCSWEGGAVKTPAGFPDAYKTYAEGGWVGVGGDPVFG
                     GMGMPKAISAQVEEMVNSANLSFGLYPMLTAGACLSINAHASEELKEKYLPNMYAGTW
                     AGSMCLTEPHAGTDLGIIRTKAEPQADGSFKVSGTKIFITGGEHDLTENIIHLVLAKL
                     PDAPAGPKGISLFLVPKFMVNADGSLGEQNSLSCGSIEHKMGIKASATCVMNFDGATG
                     WIVDAPNKGLAAMFTMMNYERLGVGIQGLSLGERSYQNAVEYARDRIQSRAPTGPVAK
                     DKAADPIIVHPDVRRMLLTMKALNEGGRAFSSYVAMQLDTAKYSEDAAVRKRAEELVA
                     LLTPVAKAFLTDMALETTVHGQQIFGGHGFIREWGQEQLIRDCRITQIYEGTNGIQAL
                     DLVGRKVVGSGGAFYKHFAEEIKAFTDSADSSLAEFIEPLKGAIANLELMTSDLLERA
                     KANPNEVGAASVEYLQVFGYTAYAYMWALMARTALAKQDQDEFYVSKLGTARFFFARL
                     LPRIHSLTASVKAGSESLYLLDAAQF"
     gene            complement(17203..18999)
                     /locus_tag="VFF22_00080"
     CDS             complement(17203..18999)
                     /locus_tag="VFF22_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HZX15252.1"
                     /translation="MPEYKAPLRDMHFLIDEVFDFHAGYAAMGASDAPPDMVNAILTE
                     GAKFCEQVLAPLNRSGDEEECQINDGVVTTPKGFKEAFAQYVEGGWNGLAADPAYGGQ
                     GLPHSLGLLISEMVGASNTSWGMYPGLTHGAMSAIHAHGTEEQKQTYLSKLTEGSWTG
                     TMCLTEAHCGTDLGLIKTRAVPQADGSYAVSGSKIFISAGEHDMSENIVHLVLAKLPD
                     APAGTKGISLFIVPKFLPNAEGQAGERNGVSCGSIEHKMGIKASATCVMNFDSATGYL
                     IGEANKGLNCMFTMMNHARLGTGMQGLCLGETSFQGAIKYATERLQMRALTGPKAPDK
                     AADPIIVHPDVRRMLLTMKAFNEGNRALAYFTAQLIDQAHRGNSAEQREEAENLLAFL
                     TPICKAFMTETGLEVTNHGMQVFGGHGFIREWGMEQLVRDCRIAPIYEGTNGIQALDL
                     LGRKVLGSQGKLLRGFTKIVHKFCEAQAEHPQLKDYVAQLATLNQQWGTLTQKIGMAA
                     MKNPDEVGAASVDYLMYSGYVTLAYFWLRMALVAEQKLAEGAGEAAFYQAKLATCEFY
                     FKRLLPRTSTHLAGAEAGSESLMQLPAEHFAL"
     gene            complement(19110..20399)
                     /locus_tag="VFF22_00085"
     CDS             complement(19110..20399)
                     /locus_tag="VFF22_00085"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZX15253.1"
                     /translation="MSRLAAVRFSHFLPSLLLLISGLLLARLAVLSEFFTSLFNVLPT
                     LLLLLGGAFCLVYGRQREVFLLLTLYLGYFLLDTQVDHFRATGQVRSDAALIFHLCSL
                     LLPLLYSLYAAWQERTHLLQDLIARAAVLLAVGAVAVGLAQRFPEALLHWLSRVRWPA
                     LQVEWLALVQLAYPVFILGLILLAVQYLRAPRPLHAALLLGLCGLLWMLPNTFILPNA
                     LNVMSSQIMLILVAAVAHEAYQMAFRDELTGLPGRRALNERLQRLGRSYVIAMADVDH
                     FKKFNDTHGHDVGDQVLRLVAAQLRKIGGGGKAYRYGGEEFTLVFPGKTLEECLPHLE
                     VVRQAIETYRIQLRDKQQRPRDDKQGRARRAGSTANQVSVTVSMGVAERGLEQRSPEE
                     VLKAADQALYNAKGAGRNCVRSYGQRRGAVKLDTTRG"
     gene            complement(20577..22382)
                     /locus_tag="VFF22_00090"
     CDS             complement(20577..22382)
                     /locus_tag="VFF22_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacyl-CoA dehydrogenase"
                     /protein_id="HZX15254.1"
                     /translation="MPDYKAPLRDIRFVRDELLGYEAHYQSLPGCQDATPDMVSAILE
                     EGAKFCEQVLSPLNRVGDTEGCTWSPEGVKTPTGFKEAYQQFVEGGWPSLAHDVDHGG
                     QGLPESLGLAISEMVGTSNWSWGMYPGLSHGAMNTISEHGTDDQKHTYLTKLVSGEWT
                     GTMCLTEPHCGTDLGMLRTKAEPQADGSYKVSGTKIFISAGEHDMAGNIVHIVLARLP
                     DAPQGTKGISLFIVPKFSVNADGSVGARNAVSCGSLEHKMGIHGNATCVMNFDGATGY
                     LIGPANKGLNCMFTFMNTARLGTALQGLAHAEMGFQGGLKYARERLQMRSLTGPKAPE
                     KAADPIIVHPDVRRMLLTMKAFAEGNRAMVYFTAKQVDIVKYSQDEAEKKQADALLAF
                     LTPIAKAFMTEVGFEAANHGVQIYGGHGFIAEWGMEQNVRDSRISMLYEGTTGIQALD
                     LLGRKVLQTQGEALKGFTKIVHKFCQVNEGNEAVKALVAPLAKLNKEWGDITMKVGMA
                     AMKDREEVGAASVDYLMYSGYVCLAYFWADMARLAAEKIAAGAEDAAFYKAKLQTANF
                     YFARILPRTRGHVEAMLSGANNLMDMAEEDFALGY"
BASE COUNT         4354 a         7051 c         6916 g         4086 t
ORIGIN      
        1 gggcatggcc cgctcctaca aatgatttag cccttttcgc tttcgagtac ccgctatgag
       61 caagatcata agcgccgccg ccgctgtggc gcgcatcccg gataacgcca acctggccac
      121 tggcggcttt gtcggcattg gtttcgccga ggaaatcgcc atcgccctgg agcagcgctt
      181 tctcacggaa caagctccgc tgggcctgac cctggtgtat gccgccggcc aaggcgatgg
      241 caagggtcgt ggccttaacc acctggccca tgagggcttg gtgcggcggg tgatcggcgg
      301 ccactggggg ctggtgccag ggctacagaa gctcgcggtg gataaccgca ttgaggccta
      361 caacctgccg cagggggtga tctcccaact gtttcgcgat atcgccgccg gtaaaccggg
      421 gcagctttcg cgggtcggtt tgggcaccta tgtcgaccca cgccacggcg gcggcaagct
      481 caatgcgtgc accaccaccg aactggtgcg gctgatgccg atagacggtg aggactacct
      541 gttctacccg accttcccga ttcacgtggc cgtggtgcgc gccaccagca gcgatgccga
      601 cggcaacctg agcttcgagc gcgaggcgct gaccattgaa agcctggcca tcgccatggc
      661 cgcgcgcaat tcgggcgggc tggtgatcgc ccaggtcgaa cgcatcgtcg aacgcggttc
      721 gctaaatgcc cgtgacgtga aaatccccgg catcctggtg gactgcgtgg tgctggccga
      781 accggccaat caccagcaaa ccttcgccac cgcctacaac ccggccttcg ccggcgaaac
      841 ccgcgtaccg atgaacagcc tgaaggccat gccactggat gtgcgcaaac tgatcgcccg
      901 ccgcgcggcg ctggagctgc aagccggcgc ggtggtcaac cttggtatcg gcatgccgga
      961 aggcgtggct gcggtggcgg ccgaggaagg cgtgatcgaa cgcctgaccc tgaccgccga
     1021 gccaggggtg atcggcggca tacccgcttc ggggctggat tttggcgcgg cgagcaacca
     1081 cagcgcgctg ctcgaccagc cttatcagtt tgatttctac gacggcggcg gcctggatat
     1141 cgcctttctc ggcctggccc aggccgatgc ggcgggcaac cttaacgtgt ccaagttcgg
     1201 ctcacgcctg gctggggccg gcggctttat caatatcagc cagaacgcca agcaggtggt
     1261 gtttctcggc accttcagcg ctgggccgca agatatccgc atcgaggacg gccagctgcg
     1321 catcatcaag gacggcgagc tgcgcaagtt tgtcgtcgag gtggaacacc gcaccttcgc
     1381 cggccaccag gccgccgagc gccgccagcc ggtgctctac gtcaccgaac gctgcgtact
     1441 gcgcctgacg ttggacggcc tggaactgat cgaagtggcg ccgggcgtgg atatccagcg
     1501 cgacatcctt gaccgcatgg acttcgcccc catcgtgcgt gaaccaaagc tgatggatgc
     1561 gcgactgttt ctgccggcat gcatcggtct gagcgaacgg ttgaccgcca actagacgta
     1621 gggtggaccc cgcttcaccg gtccaccatg ggcgaccaag ccgaatgcca atggtggaaa
     1681 taaacagcga tttccaccct acgttatcgc ggccagcgct cgggcgagga tctgctgacg
     1741 gcctttgccg cctgaatcag aacggctcaa agttgtggcg gtctatagtt ttcagtctcg
     1801 tcactcaccc ccaaggagtc gtcatgaaca agacctacag cgtcgccgta ctggtcggta
     1861 gcctgcgcaa ggcttcgatc aaccgcaaac tggcactggc cctgaccgaa cttgccccac
     1921 cttcgctgca gctgaagatc atcgagatcg gcgagctgcc gctatacaac gaagacatcg
     1981 accaggacaa cccacccgcc gcctataaag acttccgcag ccagttgcag gcggccgatg
     2041 ccctgctgtt tgtcaccccg gagtacaacc gctcggtgcc ggcaccgatg aaaaacgcca
     2101 tcgatgtcgg ctcgcggccc tacggcaaaa gcgccttcag cggtaaaccc ggcgccgtgc
     2161 tgagcgcctc gccgggggcc atcggcggct tcgcggccaa ccaccacctg cgccagtcca
     2221 tggtgtttct cgacgtgcat atgatgcagc agccggaagc ctacctcggc ggtgccggca
     2281 gtttcttcga tgaagccggc aagctcgacg atggcatcaa gccgtttctg cagaagttta
     2341 tcgacgccta tgccgcctgg gtggcgcggc atctctagca ggccacgggc tactgcgctg
     2401 gcggttgacg caactgcccg gccagacggt cgatggcagc gatagtctgg atgatatcgg
     2461 cgtggatatc cggcataccc aggcagatgc tgccagggta tgcccggtat tcgctggttt
     2521 ccgcttccag ctgcagacgt ggatcgaaca gcccttgggt aaggggttgg aaaatagtca
     2581 ctggatgctc cttgccggct acacgcaggc gtgacgattg aggtcaggag gcagtagcaa
     2641 ggggcacacg gccgcgacag cgttcttatg actagaagcg tctttaggtt ggaggccggg
     2701 agggcaaacg cgacccgccg acgccttaac agtccgccga ttgccgcgac gggtcaagcc
     2761 atcggccgcc ggacggtcaa ccctggctca gcagtggcgg catcggacaa tcgagaatat
     2821 ccgccaccgc tcgcatcaac tcggcctcct cggcgttgat ccggccgtca tgctcgatac
     2881 aacgcgccat ggccttgagc agctgtggtt tctgcaacgg ccagagcagg ctcaggcgcc
     2941 gcagcgcagg ttcaagcgcc actaaaccac tggcgctgac cgggaggatt tggtcgagct
     3001 ggaacggcag ctcactgcct gcatgcttga acgcgagctg cgcggcaggc tcatccactc
     3061 cgccggcgcg tgccagcagc gacagcacca cgcgggtttc agcggccagc tcctgcagct
     3121 gatagcgact ggtctgcgcg cgcacgcctt cgacattacg ctcgacgatg cgcagcagcg
     3181 tccattccag caaggcaacc cggccgtcgg cacgaatcag cagggccaga ttggccttga
     3241 gcgcagcgaa ctgatcggcc gccaattgct tgagcgccgg catggccaaa tccagcagcg
     3301 gcaaacgcat ccccggcgcc agctgacgca gcggttcacg catcagctca agctgggcgg
     3361 caatctcggc ggcgatacgc ggctgcagca gttgtagttg ctgcgccgct tgcttttcga
     3421 cggggtcgag taacaggccg tagagcagcg cctgggcgcc actgcagctg tgcgccgcct
     3481 cgctcagggc aatatccaaa tgcgccaggg tgctgcgcgc ggccagcaag tgcggggcct
     3541 gcggatcacc aatggcgcca atcgcctcac tcgccgaggc cgcgctcagg cccggaacaa
     3601 aggcgctcaa atcatgcaga ttgaccgccg aatcactggc cgctggcgtt gccgcgccaa
     3661 gctccgccac atgcgggtag cggccatccc aaccgggagt gatgcggcgg atgcgctgat
     3721 ccaggggcgg atgagtggcc agcatgccgt ccactgcctg gcggacgccg gcggcaaaat
     3781 acatatggct gaattcggcg gcatgcccgg cctgcagctg cgagccatat agattgccgc
     3841 cgatcttctt caaggctcca gcgatgctgt ccgggttgcg ggtgtactgc accgccgaag
     3901 cgtcggcaag aaactcgcgc tggcggctca cggcggcctt gatcagattg ccgaaaaagg
     3961 tcccggcata acccagcacc cacagcgtga cgccgatggc gagcacgatc gccaccgagt
     4021 tgtcgcgact ggaccggcta ccgcgaccgc tgctgcgcag cagaaaaccg ccaatcaggc
     4081 cgagcagcag aatgccgtgc agcaccgaca ccagccgcgt attgaggcgc atatcgccgt
     4141 ggaagatatg gctgaattca tgggcaatca cgccctgcaa ttcatcgcgg ctgagctggc
     4201 agatcgcgcc acgggttatt ccgatcaccg catcctgcgg cgtcaggccg gcggcaaagg
     4261 cgttgatccc ggcatcatcg agcacataca ccggcggcac cgaggtgccg gaagccaggg
     4321 ccatttcctc caccacattg agcagccggc gctcttcgag gttctgcggc gacagattga
     4381 tcaggcgccc gcccagccgc tcggcaatcg cctggccgcc ccggctcagc tgcagctgct
     4441 tgtacagact gcccagcagc accacgccga tcaccgccag cgctacgctg ccaaccaatg
     4501 gccaactggg cagcaggctg cctgggccat caagcaggcc caacgagttc tgatcgttgc
     4561 cgaatagctg ccagattgcc cccagcgcca gactggtcag ggtgatcagg ctaagcaccg
     4621 ccagcgccat cagaatcagc agacgcccgc tgtttcgccg ggcgcgatcc tgttgctcga
     4681 agaaattcat cgtgccgagc ccttaactta gaacgacacc ttcggcgctt cctgaatcgc
     4741 cacgctgtcg gcgaactcca gcaggctggc gtcctgagcg tggccgaagc tggcggccag
     4801 caacaccggc gggaacgctt gtttgtaggt gttgtaggcc attaccgcat cgttgaaggc
     4861 ctggcgggcg aacgctacct tgttctcggt gctgctcaac tcttcactga gctgctgcat
     4921 attcgccgag gccttgaggt ccgggtaagc ctcaagcgtc acgttcaggc gacccagggc
     4981 gctattgagc aacccttccg caccgcccag ctgcgccatg ctctgggcat tgccaggctg
     5041 cgccgcagcg gccttgagcc cggtgaccgc gttgttacgc gcggcaatca ccgcctccag
     5101 ggtctcgcgc tcatgggcca tatagccctt ggcggtttcc accagattgg ggatcaggtc
     5161 ataacggcgc ttgagctgca cctcgatctg cgcaaaggca ttctggaagc ggttacgcag
     5221 ggccaccagc tggttgtaga tgctaatcac gtaaaagatc agcgcgacga caaccgccag
     5281 gaaaatcatc gtggagatgg acatggggca atccttatcg catgcgggag agaagcagat
     5341 ggtagcggaa aagcgccgca gcgaatgccc acaggcgcac tctggcggca cgcctgaacg
     5401 tgcgtcgaca acaaaggaac ttatgtcgag ccatatcggc tgactatgcc gaagaaaact
     5461 cttggcaaaa tagaccgacc ggtctatata tttatcccat gaaaaagatc tcaacccgcg
     5521 acaaactgat tcacgccatg gccgacgcct tacagcgcaa ggggctgcat ggcgttggcc
     5581 tgagtgagct gttggaaatt gccggcgcac ccaagggcag cctctatcac cacttccctg
     5641 gcggtaagag cgagctggcg gtggcggcta tcgaccatat cagccggcat atcgacagcc
     5701 tgtttagcca gctttttgct cagcaaaccg acccactcaa ggcgctgcac aactggttgc
     5761 aaggcgcact gcagcagctg gagaacagtc agttcgagcg cggttgtccg ctggccacca
     5821 tcgccctgga aagtggcccg gaagatgtgg agattcgcgc cgccctgcag cgcagttttg
     5881 ccgccgtgcg ccaggcactg agccagcaac tgcatgccta cggctacccc agcgagcagg
     5941 cggataacct cgcagcgctg tttgtagcgc tgtatgaagg cgggctgctg caagcccgcg
     6001 tggccggcag cagcgagccg ctcaagcgcg ccgctactgc cctgttcaac ctgacccgcg
     6061 aacagtttcc gggaggagtc cgcccatgag cctcaatacc ccagccattc ttagcgaaag
     6121 cgtgcagctg ccggcactcg acggctatcc gctgaccgcc acgctgtatc acgctccgga
     6181 gccgatcgcg cagctggtga ttgccggcgc caccggcgtg ccgcagggct tctaccgacg
     6241 ctttgccgaa tacgccgcgc agcgcggcta tagcaccttg accctcgact accgtggcat
     6301 cggccaatcg aaacccgcca gcctgcgcgg ctttgccatg gattatctgg actgggcgca
     6361 cctcgacctg gcagccgccg tcgaccagca ccgtcacccg cagcgaccgc tgtatatggt
     6421 cggtcactcc tttggcgggc atgccttcgg cctgctgccg aatcatgatc aggtccaggg
     6481 cttctacacc ttcggcactg gcgccggctg gcacggctgg atgcccaagg ccgaacaatt
     6541 gcgcgtgctg accatgtggc ggctgatcgg cccgctgctg acccgctaca agggctacct
     6601 ggcctggagc aagctgggca tgggtgagga cttgccactg ggcgtgtaca aacagtggaa
     6661 gcgctggtgc agctacccgc gctatttctt cgaagacccg cagatgccgg aactggccga
     6721 gcgttttgct caggtcagca cgccgatcat tgccgccaac gccctggacg acttgtgggc
     6781 cccacccgcc tcacgcgatg cctttatggc cgcctacagc gccgcgccct atcaagcgcg
     6841 gaccatcgat tcagccgcca gcatcggcgc catcggccat atgggttact tccgcccggc
     6901 ggcgcagtcg ctgtgggatg aaacgctgaa ctggtttgcc gagctgcagg cgcaacagca
     6961 ggccgcctga acagcccata tcaaaggaga tttcgctgat gagcatgatc ccggcaggtt
     7021 ttaccccact gtttcgcagc agcccctttc tcgatttgct cgggccgatc tacaaccagc
     7081 gcagcgatac cggcctggtg attggcctgc gcgccgagga gaaacactgc aatgcgcgcg
     7141 gcgtggtgca tggcggcgta ctcagcagcc tggccgatgt cgccctaggc tacaactgcg
     7201 ccttcgccca ggagccgccc acgccaatag tcaccagcag cctgaccatc gactacgccg
     7261 gcaccgccaa gctaggtgat tggataacca tcgaaaccga tgtgcagaag gtcggcaaaa
     7321 gcctggcgtt tgccaattgc tactttgtgg ttgagtcgaa acgaatagcc cgcgccagtg
     7381 ccgtatttag cgtggtcaca gcttaggttt ggccgctcga gaagagtgct gctgttaggc
     7441 gaactacccg cagggtgagc ctactggcgc gcataaaaaa gaaaaccccg cgattgcggg
     7501 gcctttgcag acttgagtcc tgacatcctt gatctgcttg ccatcctggc agctgtccag
     7561 cgtgtccgtt ttgaatagtg tgcgcttcct gcgcgacgtc catgggcagc agattagcca
     7621 tcgcccggaa ttcggaacag tggcgatctg cagcacgctg tgtaagccaa agcctacacc
     7681 gccccctttc gagtaaaacc gagcaaaggc tcggctttat tttgtgacgt aggtcttgtt
     7741 agactcgatt ttgtcttaca aaaacaacag accccgtcaa cggcggtcat tggggccaca
     7801 catgcgctca tttcttttct tcatcggctg cctggcagcc accatcagct cactggcatc
     7861 cgcaaacgga caaaatcagc tttccaaccc caaagccgcc acacagttgt tctggaatga
     7921 gctttatggc cagggcggca gcacgctgta ttgcggtaaa gcgttcaccg aggaaagtgg
     7981 ccaactcaag gccagcccga tctacagcag caaacagctg aaaagcgcgc tgcgctgcat
     8041 aaccaaccgt caatgcggaa tcatgaaccc gcgctacgtc tttatggcgg ccgacctgca
     8101 caacctctat ccggcgatga cccgcgtgga actggtgcgg cgcaatgcgc agttcggcga
     8161 gctggatgac agcgtgccaa gcaaattcgc cgacatcggc tgcgatatga aaaccagctt
     8221 ccagctggtg gagccgcgcg acgaagccaa gggcaacgtg gcccgggcga tcttttatat
     8281 gcatgtcgag tacgacctgc ccatcgtcgg ccaggtgcag atgtacaaga agtggcacca
     8341 gatggaccca ccggacgccg aagaaaaggc gcgcaacgac aagatcgcca gcctgcaagg
     8401 cacccgcaac cgctttatcg acaatcctga gctggtcgag cagcagatca ataactgatc
     8461 gggtttagcg tcgcggctta tgcgatgaac gcagcttata gcgatcgccc ggatggatca
     8521 gccgggcgta gctgatcagg tgttcatccg accaagtccg acggatcaca ctcaggcacg
     8581 gcgaggccac ttcgatagcc aaccagtcgg ccacctgctg atccactagc actgcttcca
     8641 ccacatgctc gacatcggaa atcggacagc tgctgaccag cacctcgttg ggcgtttgcc
     8701 gggaaaaatc actgtccagg taatgcggca cccagcgcgg gttgacgaag cggtcttcca
     8761 gttggatcgg cacgccatcc tcgcggtgca ccagaatgct gtgaaacacc tccgcgccaa
     8821 ggcgcaggcc caagcgcagc gccacttcat catccgccgc cagcgcttca caacgaatga
     8881 tgtcgttgct gtaggcatgc ccacggccgc gcacttcagt ggcgatattc agcacttcgt
     8941 gcagcgatga ctcggccttg cggtcggtga caaaagtgcc caagccggcc tgacgtagca
     9001 ggtagccctt ctgtaccagg tcgcgaatag ccttgttggc ggtcattcga ctcaccccga
     9061 aatcccgcgc cagttgctct tctgccggaa tctgatgatt gaccgggtaa gcgccgctgt
     9121 ggatgcgctc cagcaggaac gcttcaatcg ccttgtagcg cggggttgta ctgctcactg
     9181 cggctccttg gggtggtccc gatgcgcgga taatagcgcg tccaccgcca ccgtaggttg
     9241 ggttgaggag cctgcgacga agcccaacgg ccaatgacag acggcatctg ttgggcttcg
     9301 ctgcgctcaa cgccaaccta cgccagcgac ggcaataccc cgctcggcaa caacgggctc
     9361 agcacgcgct cagctagcaa ggcattggca gcctcgatat ccggggcgaa gaagcggtct
     9421 tccacgtagt acggcacctg cgcgcgcaga gcctgacgcg cctgctcgag cagcggcgag
     9481 cttttcagcc cttcgcggaa atccaggccc tggcaggcgc ccagccattc gatggccagc
     9541 acgccgcggg tgttggcggc catttcccac aggcgtttgc cagcagctgg ggccatggac
     9601 acgtggtctt cctgattcgc cgaggtcggc agactatcga cgctgtgcgg atgggccagc
     9661 gccttgttct cgctggccag tgcggcagcg gtgacctggg cgatcataaa gccggagttg
     9721 accccgccat tggccaccag aaacggcggc aactgcgaca tatgtttgtc catcatcaac
     9781 gaggtacgac gttcgctcag tgcaccgatt tcggcgatgg ccagggcgat attgtcggca
     9841 gccatggcca ccggctcggc gtggaaattg ccgccggaaa tcacctcgcc ctcggcggca
     9901 aataccaagg ggttatccga cactgcgttg gcttccaccg ccagcacctc ggcggcctga
     9961 cgcagctggg tcaggcaggc acccataacc tgcggctgac aacgcagcga gtatgggtcc
    10021 tgcaccttgt cgcaggcttc gtgggaacgc cccacttcgc tgctgtcgcc aagcagatgg
    10081 cgatagatcg ccgctgcatc gatctgccca cgctgaccac gggcagcatg gatgcgcgga
    10141 tcgaagggtg cacgcgagcc caaagcagcc tcgacggtca gaccgccgca aacgctggcc
    10201 gccgcaaaca gatcctcggc ctcgaacaac ccacgcaatg cgtaggcagt cgacacttga
    10261 gtgccgttaa gcagcgccag accttccttg gccgccaagg tcatcggctg caaaccggcg
    10321 acggccaacg cttcagttgc tggcagccat tcgccgcgat aacgcgtctg gctttcaccg
    10381 agcagcacca gcgacatatg cgccagcggc gccaggtcgc cggaggcacc aaccgagccc
    10441 ttgagcggaa tatgcggata gacctcggca ttgatcaggg cgatcaacgc ctcgattacc
    10501 tgccggcgaa tgccagagaa gccgcgcgcc aggctgttga ccttgagcag catgatcagc
    10561 cgcaccatgg cgtcatccag cggtgcaccg ataccagcgg catgcgacag caccagggag
    10621 cgctggagtt tttccaggtc atcgttggcg atgcgggttg aggccagcag gccgaagccg
    10681 gtgttgatgc cataggcggt acggccttcg gcaatgatcc cgttaacgca ggccacactg
    10741 gcctcaatgg ccggataggc cgacgcagcc aggctcacgc gcaggggtgc ctggtaggcg
    10801 gcgcgcagtt gggccagggt caactggcca gggtgcaggg tgaataggct catgcatggg
    10861 ctccttgggt tgcgctaatc ataggcaggt cgaggccctg ctcgcgggcg caatcgatgg
    10921 caatttcata accggcatcg gcgtgacgca tcaccccggt gcccgggtcg ttgcgcagca
    10981 cgcgaccgag acgagcgcgg gcttctggcg tgccgtcagc gacaattacc accccggaat
    11041 gctgcgaata gcccataccg acgccgccac cgtggtgcag cgacacccag gtcgcgccgc
    11101 cggcggtatt caacagggcg ttgagcagcg gccagtcgga cacggcgtcg gagccgtcct
    11161 gcatggcctc ggtttcacgg ttagggctgg ccaccgagcc ggaatccaga tggtcgcggc
    11221 cgatcacgat aggtgccttc agctcgccgc tggcaaccat gtcgttgaag gcctgggcca
    11281 ggcgcgcacg gtccttcagg ccgagccagc agatccgcgc cggcaggccc tggaagctga
    11341 tgcgctcgcg ggccatgtcc agccagttgt gcaggtgggc gtcgtccggg atcagttcct
    11401 tgaccttggc gtcggtcttg tagatgtcct ccgggtcgcc ggacagcgcc acccagcgga
    11461 acgggccgat gccctggcag aacagcggac ggatataagc cgggacgaaa ccagagaagt
    11521 cgaaggcgtt gtcgacgccc tcctgcaggg ccatctggcg gatattgtta ccgtagtcga
    11581 aggtcggcac gcccatctgc tggaaggcca gcatggcgcg tacatgcacg gccatggact
    11641 gcttggcggc cttgaccacc aaggcctcct cggttttgcc gcgggccacg tattcgtccc
    11701 agctccagcc ggctggcagg tagccgtgac gcgggtcgtg ggcgctggtc tggtcggtga
    11761 ccatatccgg gcgtacaccg cgcttgacca gctccggcag aatctcggcg gcgttgccgc
    11821 agagggcgat ggacaccgcc ttgccttcgc tgcagtacgt gtcgatacgc gccagggcat
    11881 catccagatc ggtggcctgc tcatcgacat aacgggtctt caggcggaaa tcgatacgcg
    11941 attgctggca ctcgatattc agcgagcagg caccggccaa tgtcgccgcc agtggctgcg
    12001 cgccgcccat gccgccaaga cccgcagtga gcacccaacg gcccttgagg ttgccgccgt
    12061 aatgctggcg gccggcctcg acaaaggttt cataggtgcc ctgaacgatg ccctggctgc
    12121 cgatatagat ccagctgccg gcggtcatct gcccgtacat ggccaggccc ttggcatcca
    12181 gttcgttgaa gtgctcccag gtggcccagt gcggcaccag gttggagttg gccagcagca
    12241 cgcgcggcgc gtccttgtgc gtctcgaaca cgccgaccgg cttgccggac tggatcagca
    12301 gggtctggtt gtcttccagg cgggtcagca cttcgacgat cttgtcgtag cactcccagt
    12361 tgcgcgcggc gcggccgatg ccgccataca ccaacagctc cttggggttc tcggccactt
    12421 ccgggtcgag gttgttcatc agcatgcgca gcggcgcttc ggtcagccag ctcttggcgg
    12481 taagcgtggt gccacgggcg gcgcggattt cggtgtcacg gaaaaggctc attactggcc
    12541 tcctattctt gttaggtatg aggttctgga taaattcagg gggtcagagt caacagatga
    12601 atcaaccgcg ccgtgacctt ggctgtgcga ctgtcgatgt catgatcggg gttgagttcg
    12661 gccagatcca tcaggcgcaa cttgccgctg tcacgcacgg tttccagcag cggttcaagc
    12721 agcgccagcg gcacgccacg cgcagccggg gcgctgacgc ccggggcttc gcaggccggc
    12781 agcacgtcga tatcgatggt caggtagatg gcgtcgcaac cggcgataaa ggcctgcaac
    12841 tcggcactaa gcgccgtcag gctgccttcg cgaatctcgc ggtcttcgcg caccagtacg
    12901 ttcagctcgg cggcgcgctg gaacaaggcg cgggtattgc tggcgcggct gacgccgagg
    12961 caggcatagg cgaacggcca gccacaggcg gcgcactgct cggcgatctg cgcgaagggc
    13021 gtgccggagg aacgggcgtg agccgggtcg cgcaggtcga aatgcgcatc gaaattgata
    13081 attccgatac gtggggcggc catccgcggc gcgctgcctc cggccatatg ctcggcaata
    13141 ccctgccagc taccgaaggc cacctcatgg ccgccgccga gaacgatggg cagatgcccg
    13201 gcatccagca gcgcgcagac gttatgacct aggcgcgcct gggcgctttc cagatcgcca
    13261 tcggcgcaca gcacatcacc ggcatcatag gccgcgccct ggcgatgcca ggccagattg
    13321 gccaacgcct tgcgcatcgc cagcggacca tttaccgcgc ccacgcggcc atggttgcgg
    13381 cgcacgcctt cgtcgctggc gaaaccgagc agggccacgc caggctggct gtcagcgctc
    13441 agttcctgaa tgcgctgatg ccagcgcgag ctgtcggctt ccggatcgac gcggccctgc
    13501 cagaggtcca tggagttgcg atcagcgtgc atagctgttg gcctctttgc tgtagctaat
    13561 ggcgccgttg aagatgcgct gacgcagccg ccccggctgc accgcgtaag ccagctcggc
    13621 aggttgctgc acatcccaca ggcacagatc ggcaggggcg ccgacctcga ttcgacccag
    13681 gtcagggcgg ccgagggcgc gggcggcatg ggcggtcata ccggccaggg cttcgcgcgg
    13741 ggtcaaacgg aacagggtgc aggccaggtt aagggtaagg gtcggaaagc agatcggtga
    13801 ggtgccgggg ttggcatcgc tggccagggc catcggcact ttgtactgac gcagcagcgc
    13861 gatgggcggc agctgggttt cgcgcagggt ataaaaggcc cctggcaata gggtggccac
    13921 ggtgccggcg gcggccatgg cttgcacccc ggcctcgtcg agaaactcga tatggtcagc
    13981 cgagaggccc tgataacgcg cggccagggc gctggcgccc tgattggaca gctgctcggc
    14041 gtgcaccttg atcggcaggc cgtggcgttg cgcagcctgg aacaccaact cgcactgcgc
    14101 cgggctaaag gcgatgtttt cgcagaacac gtccaccgca tcggccaggc cttcggccac
    14161 ggccgccggg atcatctcgt tgcacaccag atcgatatag ccgtcgctgt cgtcggcgaa
    14221 ctccggcggc agggcgtggg caccgagcaa ggtggtggtg atgcgcagtg gcagcaactc
    14281 gccgaggcgg cgcgccacgc gcagcatctt taattcatcc gccgtgctca ggccgtagcc
    14341 ggatttgatt tccagggtgg tgacgccgtc gtccagcagc tcctgcaaac gcggcaggct
    14401 ctgcgccagc agctcgtcct cgctggctgc ccgggtggcg cgcacggtgc tgagaatacc
    14461 gccgccggcc ttggcgatct cggcgtagct gacgccctca agacgctgct cgaactcagc
    14521 ggcgcggttg ccggcgtaca ccagatgagt gtgacaatcc accagacccg gggtcatcac
    14581 cccgccacgc ccagcttcga gcgcaccttc agccatagct tcaggaaacg cgctggtagg
    14641 ccacagcccc gcgaccttgc catccgccac cagcagattg atcggctcag ccagcgtcgt
    14701 caggccatca aagaggcaga gctcgcgcca aagcaggcgc agggcggacg gggacatggg
    14761 actctccttg agcgaacctg tatatacaat aaagttcgac catgcagtcg tcaaccccgt
    14821 tcgtcaccga aaaatcactg ccgtgctaca accgtcacat gcccgcgctg aggcataacc
    14881 aaagaaatca ccgagattgt tcagcagcaa agtccagcag cagacgaacg gcagcgctcg
    14941 gctggcacag tctatgtgca gccataacgc acagaatgta tatacataaa taacagtctg
    15001 gatgagcatc cgtattatcg acagccacac ggcacgggcc tggaaccctc cgactggcgt
    15061 ataagcgttg gcgagatttg tagggtgcgc cgcgcgcacc gcagcagcct atgagacctt
    15121 ggtgcgcgcg gcgcacccta caacagcagg tttgcgcgcc ttcagcagca gcgcacacaa
    15181 aaaagccccg gctgtttaga ccggggcttg gtttactgca ggtaccgtta gaactgcgca
    15241 gcatccagca ggtacagcga ctcgctaccg gctttaaccg aagctgtcag cgagtggata
    15301 cgcggcagca ggcgggcgaa gaagaaacgc gcggtgccca gcttgctgac gtagaactca
    15361 tcctgatcct gcttggccag tgcggtgcgt gccatcaggg cccacatgta ggcgtaggcg
    15421 gtgtagccga acacctgcag gtactcgact gaggcagcgc cgacttcgtt ggggttggcc
    15481 ttggcgcgtt ccagcagatc gctggtcatc agctccaggt tggcgattgc gcctttcagc
    15541 ggctcgataa actcggccag gctgctgtcg gcggaatcgg tgaaggcttt gatctcttcg
    15601 gcgaagtgct tgtagaacgc accgccgctg ccgaccactt tacggccgac cagatcgaga
    15661 gcctggatgc cgttggtgcc ttcgtagatc tgagtgatgc ggcagtcgcg gatcagctgc
    15721 tcctggcccc actcacggat aaagccgtgg ccgccaaaga tctgctggcc atgcacggtg
    15781 gtttccagcg ccatatcggt gaggaaggcc ttggccaccg gggtcagcag agcaaccagc
    15841 tcttccgcgc gcttgcgcac tgcggcgtct tcgctgtatt tggcggtgtc cagctgcatg
    15901 gccacgtagc tggagaaggc acggccgcct tcattcagcg ctttcatggt cagcagcatg
    15961 cggcgcacgt ccgggtgcac gatgatcggg tcagcggctt tgtctttagc caccgggccg
    16021 gtcggcgcgc ggctctggat acggtcgcgg gcgtattcca cggcgttctg gtagctgcgc
    16081 tcacccaggg acaggccctg gatacctacg cccagacgct cgtagttcat catggtgaac
    16141 atggcggcga ggcccttgtt cggcgcatcg acgatccagc cggtggcgcc gtcgaagttc
    16201 atcacgcagg tggccgaagc cttgatgccc atcttgtgtt cgatcgagcc gcaggacagg
    16261 ctgttctgct cgcccaggga gccgtcggcg ttgaccataa acttcggcac caggaacagc
    16321 gagatgcctt tcgggcccgc tggggcgtcc ggcagcttgg ccagtaccag gtggatgatg
    16381 ttctcggtca ggtcgtgctc accaccggtg ataaagatct tggtaccgga caccttgaag
    16441 ctgccatcag cctgcggctc ggccttggtg cggataatgc ccaggtcggt accggcgtgt
    16501 ggctcggtca ggcacatgga acccgcccag gtgccggcgt acatgttcgg caggtatttc
    16561 tctttcagct cttcgctggc gtgggcattg atcgacaggc aggcaccggc ggtcagcatc
    16621 gggtataggc cgaacgacag gttggccgag ttgaccattt cctcgacctg ggccgagata
    16681 gccttgggca tgcccatacc gccgaataca gggtcaccac caacgcctac ccagccgcct
    16741 tcggcgtagg ttttgtaggc atccgggaag ccggccgggg ttttcaccgc gccaccttcc
    16801 caggagcagc cttcttcgtc gccgctgcgg ttcagcgggg cgatcacgcc gccggtgacc
    16861 ttgccggctt cttcgagaat ggccgctgcg gtgtcttcat cgaccacttc agcaagggcc
    16921 ggcaattggg cccacagctt ggacacttca aacacttcat tgagcacgaa gcgcatatcg
    16981 cgcaggggag ctttgtagtc agccatgggg caaatcctcg gacgagcaac aggtgtgaag
    17041 cagtaggtgt aaagatgtgc agggagtcta accgaacaac tttttagaca catagcgtct
    17101 gtttatgact aaatggtcac catccgtttg ctaggccaca ccccgcccca ttaacgacaa
    17161 aacccgccgg gtggcgggct ttgtcagggc tacaaccgct ggtcagaggg cgaagtgttc
    17221 ggccggcaac tgcatcaggc tctcgctgcc cgcctcggca cccgccagat gagtggaggt
    17281 gcgcggcaac aggcgcttga aatagaactc gcaggtggcc aacttggcct ggtagaacgc
    17341 cgcttcgcca gcgccctccg ccagcttctg ctcggcgacc aaggccatgc gcagccagaa
    17401 gtaggccagg gtgacgtagc cggaatacat gaggtaatcc accgacgccg cgccaacttc
    17461 gtccggattt ttcatcgcag ccatgccgat cttctgggtc agcgtgcccc actgctgatt
    17521 cagcgtcgcc agctgcgcga cataatcctt gagctgcgga tgctcggcct gggcttcgca
    17581 gaacttgtgg acgatcttgg taaagccacg tagcagcttg ccctggctac cgagcacctt
    17641 gcgcccaagc agatcgaggg cctgaatgcc attggtgcct tcatagatag gcgcaatgcg
    17701 gcagtcgcgg accagctgct ccatacccca ctcgcggata aagccatggc cgccgaatac
    17761 ctgcatgccg tggttggtca cctccaggcc ggtctcggtc ataaaggctt tgcagatcgg
    17821 cgtgaggaag gccagcaggt tctccgcctc ttcacgctgc tcggcgctgt tgccacggtg
    17881 cgcctggtca atcagctgtg cggtgaaata ggccagcgcg cggttgcctt cgttgaaggc
    17941 tttcatggtc aacagcatgc gccgcacatc agggtgcacg atgatcgggt cggcggcttt
    18001 atccggtgcc ttcggcccgg tcagcgcacg catctgcaga cgctcagtgg cgtacttgat
    18061 cgcgccctgg aaactggttt cacccagaca cagaccctgc atgccggtgc ccaggcgtgc
    18121 gtggttcatc atggtgaaca tgcagttgag gcccttgttg gcctcgccga tcaggtagcc
    18181 ggtggcgctg tcgaagttca tcacgcaggt ggccgaagcc ttgataccca tcttgtgttc
    18241 gattgagccg cagctgacgc cattgcgctc gcctgcctga ccttcagcgt tgggcaggaa
    18301 cttgggcacg ataaacagcg agatgccctt ggtgccagcc ggcgcatcgg gcagcttggc
    18361 cagcaccagg tgaacgatgt tctcgctcat atcgtgctcg ccggcggaga taaagatctt
    18421 gctgccgctg accgcatagc tgccgtcggc ctggggcacg gcgcgggtct tgatcagccc
    18481 cagatcggtg ccgcagtggg cttcggtcag gcacatggtg ccggtccagc tgccttcggt
    18541 gagcttgctc aggtaggtct gcttctgctc ctcagtgccg tgggcgtgga tcgccgacat
    18601 cgcgccgtgg gtcaggccgg ggtacatgcc ccaggaggtg ttgctcgcgc cgaccatttc
    18661 gctgatcagc agaccaaggg aatgcggcag accctggccg ccgtaggccg ggtccgccgc
    18721 caggccattc cagccgccct caacgtactg ggcaaaggct tccttgaagc ccttgggtgt
    18781 ggtcaccaca ccgtcgttga tctggcattc ctcttcatcg ccggagcggt tcagcggggc
    18841 cagcacctgc tcacagaact tcgcgccctc agtgaggatc gcgttgacca tatccggggg
    18901 ggcgtcgctg gcacccatcg cggcgtagcc agcatggaag tcgaagacct cgtcgatcag
    18961 aaagtgcata tcgcgcaggg gagctttgta ctcgggcatg ctggcatctc cgtcagctag
    19021 agcgtggatt agctctttaa gctacggctc atgcagcgtt gcaacaatca cagtgcagat
    19081 gctgaataca ctgccattag ccgacggtct taaccccgcg tggtatccag cttgaccgcc
    19141 ccgcgtcgct gaccataact gcgcacacag ttacgtccgg cgcccttggc gttatacagc
    19201 gcctggtcgg cggccttgag tacctcttcc gggctgcgct gctccagtcc gcgctcggcc
    19261 acgcccatgc tgaccgtcac cgacacctga ttggccgtgc tgccggcgcg ccgcgcccgg
    19321 ccctgtttgt cgtcccgcgg gcgctgctgt ttgtcgcgca gctggatgcg gtaagtctcg
    19381 atggcctggc gcaccacttc gaggtggggt aggcattcct cgagcgtctt gccggggaac
    19441 accagggtaa attcctcgcc gccatagcgg taggccttgc ctccgccacc gatcttgcgc
    19501 agttgggcgg cgacaaggcg cagcacctgg tcgccaacat cgtggccgtg ggtatcgttg
    19561 aatttcttga agtggtcgac gtcggccatg gcgatcacat aactgcggcc cagacgctgc
    19621 agccgttcat tcaaggcacg ccgcccaggc aggccagtaa gttcgtcacg gaaggccatc
    19681 tgataggcct catgcgcaac ggcggcgacc aggatcagca tgatctggct gctcatcaca
    19741 ttcagcgcat tgggcaggat aaaagtattc ggcaacatcc acagcaggcc gcacaggccc
    19801 agcagcagag ccgcatgcaa cggccgtgga gcgcgcaagt actgcaccgc tagcagaatc
    19861 aatccgagga taaataccgg ataagccagc tgcaccagcg ccaaccactc aacctgcaac
    19921 gccggccagc gcacacgcga taaccaatgc agcagcgcct cgggaaagcg ctgcgccagg
    19981 cccaccgcca ccgcaccgac tgccagcaga accgctgcgc gggcaatcag gtcctgcagc
    20041 agatgggtac gttcctgcca ggccgcgtag aggctgtaga gcagcggcaa caacaggctg
    20101 cacagatgga agatcagcgc cgcatcactg cgcacctgcc ccgtggcgcg gaaatgatcg
    20161 acctgggtat cgagcagaaa gtagccgagg tacagcgtca gcagcaggaa cacctcgcgc
    20221 tgacggccat acaccaggca aaacgcgcca ccgagtaaca gcaggagggt gggcagcaca
    20281 ttgaacagcg aggtgaaaaa ctcgctcagc accgccaggc gcgccaacag caagccgctg
    20341 attagaagca gcagtgaagg cagaaaatgg ctgaagcgca cagcagcaag acgcgacaag
    20401 acgggctcca cacagcaata aaaagtggat aacaccgcgc agtttgcctg taacgagagt
    20461 gtttcgcacg aaacctcgca ggcaaatcga caagctatct aagacttttt tgtgcgccca
    20521 aataaaaaac cgccacccaa agggcagcgg ttttttgacc tggaaaggac taaggcttag
    20581 tagcccaggg cgaagtcttc ttcggccatg tccatcaggt tgttggcgcc agacagcatg
    20641 gcttcaacgt ggccacgggt gcgcggcagg atgcgtgcga agtagaagtt ggcagtctgc
    20701 agcttggcct tgtagaaagc cgcgtcttca gcgccagcag cgatcttctc ggcagccagg
    20761 cgagccatgt cagcccagaa gtaagccagg cagacgtaac cggagtacat caggtaatcc
    20821 accgaagcgg caccgacttc ttcacggtcc ttcatggcgg ccatacccac tttcatggtg
    20881 atgtcgcccc actctttgtt cagcttggcc agcggtgcaa ccagcgcttt gacagcctcg
    20941 ttgccttcgt tgacctggca gaacttgtgc acgatcttgg tgaagccttt cagcgcttcg
    21001 ccttgagtct gcagcacttt acggccgagc agatcgagtg cctggatacc ggtggtgcct
    21061 tcgtacagca tggaaatacg gctgtcgcga acgttctgct ccatgcccca ctcggcgata
    21121 aagccgtggc cgccatagat ttgtacgccg tggttggcgg cttcgaagcc gacttcggtc
    21181 ataaaggctt tggcgattgg agtgaggaaa gccagcagtg cgtcggcttg cttcttctcg
    21241 gcttcgtcct ggctgtactt gacgatgtcg acctgcttgg cagtgaagta caccatcgca
    21301 cggttacctt cagcgaaggc tttcatggtc agcagcatgc gacgcacgtc cgggtgaacg
    21361 atgatcggat cagcggcttt ttccggcgct ttcgggccag tcagggagcg catctgcaga
    21421 cgctcacggg cgtacttcag gccgccctgg aaacccattt cggcatgagc cagaccttgc
    21481 agagcagtac ccaggcgagc cgtgttcata aaggtgaaca tgcagttcag gcctttgttg
    21541 gctgggccga tcaggtaacc agtggcaccg tcaaagttca tcacacaagt ggcgttaccg
    21601 tggataccca tcttgtgctc gagcgagcca caggaaacgg cgttacgcgc accgacgctg
    21661 ccgtcagcgt tgaccgagaa cttcggcacg ataaacagcg agatgccttt ggtgccttgc
    21721 ggcgcgtcgg gcaggcgggc cagcacgatg tggacgatgt taccggccat gtcgtgttca
    21781 ccagcggaga taaagatctt ggtgccggaa accttgtacg agccatcagc ctgcggctcg
    21841 gctttggtac gcagcatgcc caggtcggtg ccgcaatgcg gctcggtcag gcacatggtg
    21901 ccagtccatt cgccggatac cagcttggtc aggtaggtgt gcttctggtc gtcggtaccg
    21961 tgctcggaga tggtgttcat cgcaccgtgc gacaggcctg ggtacatgcc ccacgaccaa
    22021 ttagaggtac caaccatttc gctgattgcc agacctaggg actcaggcag gccttggccg
    22081 ccgtggtcaa cgtcgtgggc caggctcggc cagccgcctt caacgaactg ctggtaggcc
    22141 tctttgaagc cggtaggggt tttcacgcct tccggggacc aggtgcaacc ttcggtgtca
    22201 cctacacggt tcagcggaga cagcacttgc tcacagaact tggcgccttc ttcaaggatg
    22261 gcgctgacca tgtccggagt agcgtcctgg cagccaggca ggctctgata atgcgcttcg
    22321 tagccgagca gttcgtcgcg tacgaaacgg atatcacgca agggggcctt gtaatcaggc
    22381 atagcggtta acctctgcgg tggattc
//
LOCUS       DASRUM010000002        40287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1171132, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000002 DASRUM010000000
VERSION     DASRUM010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 40287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40287
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171132"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFF22_00095"
     CDS             <1..199
                     /locus_tag="VFF22_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014755346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
                     /protein_id="HZX15255.1"
                     /translation="LAVLSCGHTQHLRHQPPWQNRAWVLDAHQRQAHVGGPFLCGWCA
                     AEKGASEALTERPNQDLNKER"
     gene            200..1387
                     /locus_tag="VFF22_00100"
     CDS             200..1387
                     /locus_tag="VFF22_00100"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HZX15256.1"
                     /translation="MPARNILVINCGSSSIKFALINEAQDAFILSGLAERLGSPDAQV
                     HWQQGNSKDSLILPHADHRAALAQVLPLVQAAAGGQLHGIGHRVVHGGEHFTAACRLD
                     ALSLAAIRATAPLAPLHNPANLLGIEAAIKLFPSLTQVAVFDTAFHQTLPEHAYRYAL
                     PDHLYREHGVRRYGFHGTSHRFVSQRAAEMAGLPVNASSWLVAHLGNGCSTCAIVDGQ
                     SRDTSMGLTPLEGVVMGTRSGDVDPNLHSHLSRTLGWSLEQIDAMLNKDSGLLGLSGL
                     SNDMRTLEQAREQGHAGATLAIEVFCYRLAKSLAAMSCALPQLDGLIFTGGIGENSAL
                     IRSKTVTHLKLLNLALDEPANARTVRGSAGPIQADGHPRVLVVPTNEERQIALDTLAL
                     LEA"
     gene            1415..3508
                     /gene="pta"
                     /locus_tag="VFF22_00105"
     CDS             1415..3508
                     /gene="pta"
                     /locus_tag="VFF22_00105"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HZX15257.1"
                     /translation="MHTFFIAPTGFGVGLTSISLGLVGGLERAGLKVGFFKPIAQPHQ
                     GDMGPERSSELIARTHGLHSPKPLALAHVERMLGDGQLDELLEEIISLYQQAAAGKDV
                     VIVEGMVPTRQASYAARVNFHLAKSVDAEVILVSAPEQESLSELSDRVEIQAQLFGGP
                     KDEKVLGVILNKVRSNDGIEAFATRLKELSPLLKGDDFRLLGCIPWQDELNAPRTRDI
                     ADLLGARVLNAGDYEQRRMLKIVLCARAVANTVQLLKPGTLVVTPGDRDDIILATSLA
                     AMNGMPLAGLLLCSDFAPDPRIMELCRGALQGGLPVMTVSTGSYDTATNLNRLNKEIP
                     VDDKERAEKVADFVASHIDHEWLFARCGTPRELRMSPPAFRYQLVQRAKAANKRIVLP
                     EGSEPRTVQAAAICQARGIARCVLLAKPEDVHAVARAHGIELPEGLEILDPDLIRERY
                     VAPMVELRKGKGLNAPMAAAQLEDTVVLGTMMLALDEVDGLVSGAINTTANTIRPALQ
                     LIKTAPGYKLVSSVFFMLLPDQVLVYGDCAVNPDPTAVELAEIAIQSAASASAFGIIP
                     RVAMLSYSTGDSGSGEEVEKVREATRLAKALDPQLLLDGPLQYDAAAIESVGRQKAPG
                     SPVAGRATVFIFPDLNTGNTTYKAVQRSADCVSVGPMLQGLRKPVNDLSRGALVDDIV
                     FTIALTAIQAANLPQ"
     gene            3708..4613
                     /locus_tag="VFF22_00110"
     CDS             3708..4613
                     /locus_tag="VFF22_00110"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009867899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZX15258.1"
                     /translation="MLHFLPAPLRGLLAGLLLALNTLICCWPLFIGALLKFLLPIPKV
                     QRALRAAMHATAEFWISVNKFWMHLVGKTEWNVEGLKGFDMQHSYMVTSNHQSWVDIL
                     VLQYQLNRRIPFLKFFLKQELIWVPVIGLCWWALEFPFMKRFSKEYLAKHPEKRGQDL
                     ATTRKACVRYKSNPVSVFNFLEGTRLSPTKHAQQQSPFKHLLKPKAGGIAFVLDAMGE
                     QLHSLVNVTIHYPQGNPGFWDLLCGRVEQVVVRFDQLQIPEEFIGSNYDQDEAYRLQF
                     QQWVNQLWLDKDAQLDELKKRYPAR"
     gene            complement(4643..6793)
                     /locus_tag="VFF22_00115"
     CDS             complement(4643..6793)
                     /locus_tag="VFF22_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZX15259.1"
                     /translation="MAHVQLKVATSTQILLVVDDRPENLEAMQALLEEGDWQLRCVDS
                     GEAALQCLLEEDVGLVLLDVQMPHMDGFEVARLMRGNPRTRYTPIIFVSAISQTQDSV
                     IKGYATGAVDFMLKPFDSNVLRHKIHALLEHERSRSELLQLTQQLDTARAFNASVLDN
                     AAEGIMVVGEDGRINFANPAMARMLEGSINDLEGSEMLSYLVSPQVTSDWRQSEFYLQ
                     WRRGEAYRLHDASLRTLNGGQVPVALSCSPLPRLQHAMVVMALDMSVVRRLHQQLESQ
                     AITDALTGLLNRRGFYQALESALSRINRSGQRMAVLYLDLDGFKRINDSLGHDVGDRL
                     LKLVGEQLKASLRPYDSLARIGGDEFTALLDTLEHPEDAARIAEKLIEQVSVRHNLDG
                     IEVTLGASVGIACFPECGQTVEGLLRAADMAMYEAKRTGRQQYRFFSPEMNGRARSRL
                     MLEESLRSAIENNDFVLVYQPQIYLDSGRLRGFEALLRWQHRVAGTVAPGVFIPLLEE
                     TRLINRLGSWIFQTGIEKCQQLSQQYGNELVLSLNVSPVQFGMPQLVEDLRRVLEEHQ
                     LNPRQLEVEVTESALMQDLETSQEQLRQLRQLGVKIAIDDFGTGYSSLAYLRHFELDT
                     LKIDRLFISNMLDSPRDAAVVSTIIDLGRNLGLEVIAEGVETMAQRDWLAAHNCAIMQ
                     GFLVAPGLSTEQIKTFPAQLDWHNLPLRNEKPVG"
     gene            complement(6866..10621)
                     /locus_tag="VFF22_00120"
     CDS             complement(6866..10621)
                     /locus_tag="VFF22_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX15260.1"
                     /translation="MDDRPASTSGKPWLPLWVTLAVLIGLGTLIVWQWQGFEQRELEQ
                     NQQRFELEAQDIGQRVISRMQAYEMVLRGVSGLMTGSDHVSLTEWERALDQLQLQDRY
                     PGILALAWSRYLPAEQLDAYLLEQQSQGRADYRLFPAGARQHYLLIDYISPLDWRNRR
                     VLGYDMLSEAVRRTAVEQAIDTGQAILSAPVTLRQETEVNVQGGILLYLSVYQPGMPQ
                     STAEERRAALLGVVHGAFRSHELMEGILGAQSQRFDVVIKDQQALQTPLLSAEIQGVA
                     QQPRFQHELELPLYGRNWLLRVTSTPAYEAAFSSRGISLWIGLVAAALLALLVGGYLY
                     LRERELHANHLATDKLREREERFRLLVERLPVATLLCNAQGRIEMANRSAADLLDSTP
                     DGLVGERVKRFLPSVEQLSALAIDHDTSELSNEYLARRDLGEEVPVALSLSVLGDSGG
                     VNYLINLIDLRARKGAEERFRLVVEASPNAIVLVDNKGCIAMVNRQTEQMFGYGREAL
                     LSQPVEILLPTALRGSHVPMRQGYQKSPEPRRMGNNRELFGQHRDGRQIPLEVGLSPI
                     SGGEGALVQAVIIDISERKAAEQRLRDQAEQLILANRYKSEFLANMSHELRTPLNSIL
                     ILSDQLRLNSAGNLTEKQAKHADIVHRAGSDLLQLINDVLDLAKVESGRMQLKLEPLN
                     MQDVLVELDASLRPMAEIKGLRLHTQLEAGVPRVIHTDRIRLQQILRNLLSNALKFTE
                     HGEVSLTVSCASGEPEEGHELLNFSVRDSGIGIPAEQHEQIFQAFQQIDGSTSRRFGG
                     TGLGLAITRQLVLAMDGEISLQSALGEGSCFTVQLPVRVVAQAQAEPEVDAPQRSGEG
                     PAVLIVEDDVNFAVVLAEEAHAHGFASVHCRSGVQALNLLQSERFSAVILDILLPDIS
                     GWQLFRRLRSHASHRSTPVHIISCVPQPVDWNDDGTRYLVKPIQRADLEQVFVDLQQL
                     DQSPAQRLLLVEDVEVEREHYREHLQQLGFDVHACARADEAQQAYASGMFNALVIDLD
                     LPDQDGFELLETLNRQRPLNGTRVVINTGVDVTRQALQRLRRYSAVVVHKHGEDMQEL
                     SEAVQGFLGSVRDPSRAGAPRIENPLEGRRVLLVDDDVRNVYALTALLDEVGLIVSSA
                     KDGQEAINCYEREPFDLILMDMAMPTMDGYTATRLLKTEHGCSIPIIALTAHAMKGDR
                     EKCITAGADDYLAKPVSREALLELLVRWLTTAEPARRSFEVPAVPPQ"
     gene            complement(10789..11445)
                     /locus_tag="VFF22_00125"
     CDS             complement(10789..11445)
                     /locus_tag="VFF22_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZX15261.1"
                     /translation="MKLWRNLAVVTLGLGILAGCTTNPYTGEREAGKAGIYGGVGAVT
                     GAVIGAATSSKKDRAKGALIGAAVGGAAGGGYGYYVDTQEAKLRQTLQGTGVQVQRNG
                     DDLKLIMPGNITFASNSADISSSFYPTLNSLVTVFKEFNKNGIDIVGHTDSTGSLELN
                     QNLSNRRAQSVASYLSANGVPASRLSSYGAGPNQPIASNASEAGRAQNRRVEINLRPL
                     "
     gene            complement(11546..12193)
                     /locus_tag="VFF22_00130"
     CDS             complement(11546..12193)
                     /locus_tag="VFF22_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZX15262.1"
                     /translation="MSSAATPTLIRETFPVGPLHCNCTIIGDPVTKKAIVVDPGGNPE
                     LIMARLEAHGLKVVSIIHTHAHLDHFLASGQMKEKTGATLHLHKEDQFLWDNLEMQCR
                     MFGVPYTPVPAPDQWLSDDEELACGCGVALHTPGHTPGSMSFWFPQDKLLIAGDTLFK
                     RGIGRTDLWGGDYPTIERSIKQRLYTLDEDAIVVTGHGADTRLGDEMRDNPFIRA"
     gene            complement(12196..13215)
                     /locus_tag="VFF22_00135"
     CDS             complement(12196..13215)
                     /locus_tag="VFF22_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017519109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZX15263.1"
                     /translation="MKPSLSFSAELVPVAYAAALLDLVEELGVPRAQLFAEARVRPEV
                     LGNPQGRLSFIDFTQLSSSALRLCDEPALGLLLGQRLNVSTHGILGYAVLSSANLGKA
                     IQFALKYYRILGLTYELEMVELGTRIELRASETMPLGPLSRFAAEGLLTSCFTIVRFL
                     VGERLEDVDVGFAHAAPHYAERYERLFGVPVVFDQPYHRLSMPITYLDRPMALANPAT
                     VQMCEQQCEALLVSLDVQDGLLTRVRRLLLTRPGDFPSLASAAEALHTSGRSLRRHLS
                     TMGTSYQQVLDEVRKSLALQYLTTTHLPLYEIAYLLGFSDPSNFRRAFKKWTGKLPSD
                     YRAEE"
     gene            13611..14450
                     /locus_tag="VFF22_00140"
     CDS             13611..14450
                     /locus_tag="VFF22_00140"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZX15264.1"
                     /translation="MVSTTPEGLQIRPRHMDFDFPNPLPRHWHSGDAFKSHLFDAMSV
                     LFPDGERFFIDSVRHFRDQIDDPVLKEQIRGFIGQEGHHSREHLEYSNRLRDLGYNIT
                     RIEKRARARIRFAQKKLSPQRQLAATAALEHITAIMADGLLKNPKYLAGAHPTMVRLW
                     RWHALEETEHKAVAFDVYNQVCGSRKLLRRAMIMASFLFVLDTTRGLAHMLKQDGLLW
                     NWRVWRDGIQWMWGKEGVFRPLIGTYRDFFKSNFHPWEHNNLELLYQTRSEFDLPAQS
                     QAA"
     gene            complement(14553..15494)
                     /locus_tag="VFF22_00145"
     CDS             complement(14553..15494)
                     /locus_tag="VFF22_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX15265.1"
                     /translation="MSASSLPLMPPLAHGFARLGFGALPLERLKARYGNATKGSRYVE
                     LDGFNLHYRDEGSRDKPVLVLIHGVVASLHTWDDWLPAFAADYRVIRFDVPGFGLTGP
                     ARDGVYSAERMIKVFGLLLDYLGVNKASIVGNSLGGYIAWNFALAQPQRVEKLVLIDP
                     AGYHMHKVPWMIAAAALPGATVAMPLWMPRALIAQGIKEVYGEPARIKPGVVDRYNDL
                     TRRPGNRRAMMDIFRVLLKVNKEELYGTAARVAQIQAPTLLLWGERDRWISPKHVPLW
                     QRDLPGIQVKTYPGVGHIPMEEIPQQSAADALRFLQG"
     gene            complement(15574..16254)
                     /locus_tag="VFF22_00150"
     CDS             complement(15574..16254)
                     /locus_tag="VFF22_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZX15266.1"
                     /translation="MQQTLAELGAVRAVFEDLGGYGAVAQQQEQDGDGPLIGHLQRTN
                     IQLAAIPVFARKGLAETTVNDLLAAANVSRRTFYKYFENKMQVLEGIYQTAVSLMLLR
                     FSEMQTLAGSPQAWLQGMVGRYFDYHLAVGPIIRLMQEEALRTDSPLAAHRQRAHTEM
                     ARLLADRLHGATHTTDPLTYRALIWAMEAASLDLLGRAASREEIEQAKRVLSELLIAS
                     LCPASSST"
     gene            16342..17208
                     /locus_tag="VFF22_00155"
     CDS             16342..17208
                     /locus_tag="VFF22_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15267.1"
                     /translation="MNTLTRRLCLPLAILLLVLLSPVQAAELFYLGQKIPDIHKTWNS
                     ADYQQLLEALNKVDSTQLNALPRRSGEFTGPIYERMVSEENFRPQLNIYAPLELRQNE
                     AREALFRLKELMRLYFDFRAVQQPYAAEALGLMSYSLRQQAVLFTLTTEFWMTLSESE
                     QSNPVRLQGLHETKAAAAMLTSSAFDYLGLTKQFGRDELVLYSAELSKQLPELFIHLS
                     ADVRETLLQRVEQLAQQHAYAEVQQNMRELQPVLLSIQQDVQAKLAAAAARPRKAPTR
                     SLDLSVPEESAQ"
     gene            complement(17197..19920)
                     /locus_tag="VFF22_00160"
     CDS             complement(17197..19920)
                     /locus_tag="VFF22_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZX15268.1"
                     /translation="MTDLSRTPGLVAAPNVLGGHFYRPPLPASYVARPGLCERLLGGL
                     AGRLLLVSAPAGFGKSSLAIEFCEHLPEQWQSLWLGLSARDSDPGRFLERLLCGLQQF
                     FPGVGQEALGLLRLRQRHQPFAFEEWLDGLLDELALRLDPQQPLLLVLDDYHLAQGAV
                     LDRCLQFFLSHLPPGLVMLVTSRQRPDWHLARLRLSRQLLELHEQDLRLTDTEGTELL
                     ALQGAVLSDDALAVLLQRSEGWVAGLRLWSLAASEVQESSALPTLAQGADGLIHDYLL
                     EEVIERQPSEVQAFLYDTAGQERFCAELCDALRDAHDSAAILRHLQAHQVFLVPLDEQ
                     GKWFRYHHLFSDLLRSRPQPTPGLHLRACRWFSAEGLLDEAIEQALRAGQPDVAANLV
                     QNLSEEQLLAEQNVATLLRWKMDLPDSLLTSTPRLILLYGWALALACQLDAAEELLAQ
                     LGRFLPARQRCEQEGLLAQWLALSGVIARGRGDSAKAQAYCSEALSCLPLDRYGQRLM
                     CLSSLANLAIVHGDLWRARGLNREALELAQRVGNPLFEALAHYDRARVLQARGEVLRA
                     LDEVRQGQQRLASLPAQRQYAARGRLTLYEGYLLSLRLQPQQARQRLLAGIAEARSCR
                     DISVLIGYCVLASMEGRSGNLPEAFAQLAEAERLMHIWDIPPIYYLAMITLTKCELWL
                     RQGQTELAGVWLSRLLDAYGGEQAAAAPEFHPQLPLHIQLHLATYERLQGEDEQAERR
                     LRGLSQLAQNAGGQLLGAIAQMQLTALLRDGGRERDAQQQLRSALQAAAGGGLLPFHG
                     LLVRQPEWLREQLLTHEVSPLVEALLQHLPQSVQAFSQDDYAGVVDALSSRELSVLRL
                     IAQGCSNQEISDSLFISLHTVKTHARHINSKLGVERRTQAVARAKSLGLLR"
     gene            complement(20199..21566)
                     /locus_tag="VFF22_00165"
     CDS             complement(20199..21566)
                     /locus_tag="VFF22_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HZX15269.1"
                     /translation="MKTTKSLLQTGALALSLLASGVMAAVSPDEAAKLGTTLTPVGAE
                     MAGNADGSIPAWTGGLPASAGTADGAGFLSDPFPSEQPLFTITAQNVDQYKDKLTPGQ
                     LAMFKRYPESYKMPVFATHRTATMPDFILAAAKNNAVNTKMVEGGNGLENFEQANPFP
                     IPKDGLEAIWNHITRYRGGSVKRLVTQATPQPNGSYSLVYFQDEFTFRGALKDADTSK
                     PSNVLFYFKQRVTAPSRLAGNVLLVHETLNQVKEPRLAWLYNAGQRRVRRAPQVSYDG
                     PGTAADGLRTSDNFDMYNGAPDRYEWKLNGKKEIYIPYNSYRLDSPKLKYDQIIKAGH
                     INQDLTRYELHRVWNVTATLKSGERHIYAKRDFYLDEDTWQAAAIDHYDGRGTLWRGA
                     EAHAQYYYDKQVPWYSVETLYDLLSGRYLALGMKNEEKSAYEFDYPAKESDYTPAALR
                     QAGVR"
     gene            complement(21597..23486)
                     /locus_tag="VFF22_00170"
     CDS             complement(21597..23486)
                     /locus_tag="VFF22_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HZX15270.1"
                     /translation="MTKTKQTWRLAKLPLAVSLASTLAAPAFGVTFNIGEIEGQFDSS
                     LSVGASWSVRGADPDLIGSNNGGDGLSQTTDDSRQNFKRGETFSKIFKGIHDLELKYG
                     DTGVFVRGKYWYDFELKDESRLFKDISDSNRKEGAQASGAQILDAFVYHNYAIADQPG
                     SVRLGKQVVSWGESTFIQNSINSINPVDASAFRRPGAEIKEGLIPVNMFYVSQSLTEN
                     LSMEAFYQLEWDQTVVDNCGTFFAQPDIIADGCTDNLRVLSNNPAAGAGTNAALVGLF
                     GRPDLVIDQNNEGVLVARSGDRDARDSGQFGTAFRYFSPELDTEFGAYFMNYHSRTPA
                     FSAAGPAADVYAVAGLLGAAAPLVVAGNSNYFLEYPEDIQLYGLSFSTTLPTGTSWAG
                     ELSYRPNAPVLLNTTDILFAGVNPLGGLFGNASLIDGQPGQEVNGYKRKEITQFQTTF
                     THFFDQVMGASRLTLVGEVGIVHVGGLESSSDTRYGRDPIYGPGPLPDTQVGPATFIN
                     TCNALNGSTLGTASRENQSKYCEDDGFVTSTSWGYRARAIWDYPDVFAGVNLKPSVSW
                     SHDVDGYGPNGLFGEGNKAISLGLDAEYQNTYTASLAYTDFFGGKYNTSIDRDFVALS
                     FGVNF"
     gene            complement(23858..25540)
                     /locus_tag="VFF22_00175"
     CDS             complement(23858..25540)
                     /locus_tag="VFF22_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HZX15271.1"
                     /translation="MLQTRLIAPADNAHQAPLLIKRLLLSGIRYEKTREIVYRDQLRY
                     SYATLNERVARLANVLSEAGVKAGDTVAVMDWDSHRYLECMFAIPMIGAVLHTINIRL
                     SADQILYTMNHADDKFVLVNSEFVPLYKGIESQLTTVEKTILLTDAAEKTADLGGLVG
                     EYENLLAAASPRYDFPDFDENSVATTFYTTGTTGNPKGVYFSHRQLVLHTMAEASVLG
                     SLDSIRLLGTNDVYMPITPMFHVHAWGIPYVATMLGIKQVYPGRYEPDMLCRLIKEEK
                     VTFSHCVPTILQMVLAAPGAQGHDFGGLKMIIGGSALNRSLYETAKSRGIQLTAAYGM
                     SETCPLISCAHINDELMAGSEDERTTYRIKAGVPVPLVEAAIMSADGTLLPADGESQG
                     ELVLRAPWLTQGYFREPEKGAELWEHGWLHTGDVATIDDFGFIDIRDRIKDVIKTGGE
                     WISSLELEDLISRHPAVREVAVVGVVDPQWGERPFALLVLREGQSLDAKGLKEHLKPF
                     VDQGSINKWAIPSQIALVTEVPKTSVGKLDKKRIRLDIVQWQAAGSPFLSTV"
     gene            25723..26898
                     /locus_tag="VFF22_00180"
     CDS             25723..26898
                     /locus_tag="VFF22_00180"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZX15272.1"
                     /translation="MSDIVIVSGARTPMGGFQGSLSSIAAVDLGAAAIRAAVERAGVA
                     PADVQEVIMGCVLPAGLKQGPARQASLNAGLPAATGCTTINKLCGSGMKAVMMAFDSL
                     KAGSNNVMIAGGMESMSNAPYIMSKARAGLRMGHGEIKDHMFLDGLEDARTGRLMGSF
                     AQETADQYGITREQMDAYAIESLKRAQAAIASGALDAEIVPITVSGRKGDVVVKDDEQ
                     PLNANLEKIPGLKPAFRKDGSITAANASSISDGASALLLMTAEEAAKRGLKPLAKIVA
                     HATQSQDPSEFTIAPIGSISNLLKKTGWDKAEVDLYEINEAFAMVTMLAIREHGLDHA
                     KVNVYGGACAQGHPVGSTGSRIILTLINALKNTGGKRGIASLCIGGGEATAVAVELI"
     gene            complement(26978..27589)
                     /locus_tag="VFF22_00185"
     CDS             complement(26978..27589)
                     /locus_tag="VFF22_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2846 domain-containing protein"
                     /protein_id="HZX15273.1"
                     /translation="MRGLLWFTVLLLVTLSGCSTPGAFFGATEGEAFREHALSDDNHA
                     LVYLYRPQSDWADQELEAPALFLNHARIGSLPSNAYMVLEFDVASYQLEMRRPLFGTH
                     WTFFADGPLDFTLISSFTLDAEAGGIYYLRYDELNPPAVNEQAPSQGNGPLQLVSQEV
                     AATEIATTREIQPFEQIAASGETERMQRSFWRKVGKSLDKIGI"
     gene            complement(27679..28836)
                     /locus_tag="VFF22_00190"
     CDS             complement(27679..28836)
                     /locus_tag="VFF22_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HZX15274.1"
                     /translation="MPHNVFPWRCGNTLTLLIDGPQFFVQMLARIDAAQRQVELELYL
                     VESGACGSALLESLCAAAQRGVAVRCLLDAFGAQGLYSADRQCLQAAGVQVQWYNPLR
                     WKHGLRNFHRDHRKLLLVDGQFAFVGGTGATDEFWQPEKASSPWHEVMVEISGPLVAD
                     WQQLFERQWQASRDWIVWRPRMPLRLKNLPALPALGQGLGRVAYADASQYRDILLSLV
                     RALRGSKQRIWLATPYFLPTWRVRRTLRQAAKRGMDVRLLLAGHHTDNPPVRFAGQRY
                     YSTLLKAGVRIFEYQPRFLHLKMVLVDDWVSVGSCNFDHWNLRFNLDANLEALDPTFT
                     ASVAASFQADFADSQEITLAEWRARPWWRRLQQSIWGALDQLLVNLLDRRR"
     gene            complement(28949..29287)
                     /locus_tag="VFF22_00195"
     CDS             complement(28949..29287)
                     /locus_tag="VFF22_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017519679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZX15275.1"
                     /translation="MIRRRPFSSLLLLIVLLLVALAWQNRAHLQAFPGIIGAYSAKEY
                     CSCRYVANNPADYCEAYVAQYVSLSGFFDDQANKRVTARGLGSTQSAQWLGLRQGCQL
                     LPKATALPES"
     gene            complement(29284..30408)
                     /locus_tag="VFF22_00200"
     CDS             complement(29284..30408)
                     /locus_tag="VFF22_00200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZX15276.1"
                     /translation="MSFTLFPARRLILACAFGLSCATAHAENWPGEDWDSQLAAPSSA
                     LSELESYAFPVRDDASRTGVRTDALLVIRDGQVVYERYAAPTTAATPHLTWSMAKSLL
                     ATTMGVAYGQGRFRLDAPVADYYPPFAAHPQVKVGHLLNWASGLDWQEDYEYAPLKSS
                     VVAMLYTRGRSDMAAFTAAHSADAEPGERFRYSSGDSNVLSAALKNMVGAEAYTDYPW
                     TALFDPLGIKTAVWERDAAGTFVGSSYVYMSARDLARVGLLMQRDGRWGEQQLLPKAW
                     VEFNRTPFANYQPQAEQPGEAVPGGHWWLNAAVAGTSRPWPDAPEDTLVASGHWGQGL
                     YVIASENLLIVRYADDRDGSFDRNHFLKLVLAALADEVQP"
     gene            complement(30529..31314)
                     /locus_tag="VFF22_00205"
     CDS             complement(30529..31314)
                     /locus_tag="VFF22_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZX15277.1"
                     /translation="MRREPIVLFDNGEHQCMMFDDLVSGEGVQSNQFLITDNEQYLLL
                     DPGGDLTYTPLSLELSKHIPVQDLTYIFASHQDPDIIASLDKWLLHTRARVICSKLWA
                     RFLPHLTANYLALSRGINTFDRIIALPDRGQSFALGKCTLKAVPAHFLHSVGNFQLYD
                     PVSKILFSGDMGASMVDDAYPVSDFVNHVPNMLGFHRRYMASNKICRLWAAMVRDMDV
                     AMIVPQHGRPFVGAEMINAFLYWIESLECGMDLMGPEDYRLPK"
     gene            complement(31402..32289)
                     /locus_tag="VFF22_00210"
     CDS             complement(31402..32289)
                     /locus_tag="VFF22_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZX15278.1"
                     /translation="MPWQQLLSPQARLPVAGLEEWYASLLERIGNPPPLQLALLGGRL
                     AATPGLAFLAGYQGALRALWPAAPWTLGALCVTENRSTRPADMSTRINALTLDGRKDF
                     VTAAEAADWLLVAAREETGGAPVQLALGVVRNGAPGVQIETLPALPLMPDVSHGRLHL
                     QGAQCERLAGDGWDDYVKPFRTLEDTHVLAALAAWLFSVGQESAWPQALQLRLLGLLA
                     GCAEVARQCPSAADSHLMLAGLFAQFDSLRSDLDNAFTAGDAHWAPLWQRDKGLLAIA
                     GSARSKRLQKAQAVLGINL"
     gene            32438..33187
                     /gene="olsB"
                     /locus_tag="VFF22_00215"
     CDS             32438..33187
                     /gene="olsB"
                     /locus_tag="VFF22_00215"
                     /EC_number="2.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ornithine N(alpha)-acyltransferase"
                     /protein_id="HZX15279.1"
                     /translation="MTPMALKATPARRLQAERLHGAAALREAQALRFRVFSAEFDAKL
                     KGAELGLDMDDYDIHCQHIGVRDLNSGELVATTRLLDHRAAASLGRFYSEEEFSLHGL
                     PHLEGPVLEIGRTCVDAAYRNGATIAVLWGELAEVLNEGGYRYLMGCASISMQDGGIQ
                     AQAIMQRLRERYLCTEHLRAEPKTPLPALDVPSNVIAEMPPLLKAYMRLGAKICGEPC
                     WDQDFQVADVFILLKRDELCPRYARHFKAAV"
     gene            33400..34194
                     /locus_tag="VFF22_00220"
     CDS             33400..34194
                     /locus_tag="VFF22_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZX15280.1"
                     /translation="MKKLRLYLRLARLLAVISLGTLLAAGITLLERLVRHDLMPTRQR
                     LTRWFLARLAAALPFRLQVQGELPRQPMLWVSNHVSWTDIPLLGMLTPLSFLSKAEVR
                     TWPVAGWLAHKAGTLFIRRGSGDSNLLNQQLGNHLQRGRNLLIFPEGTTTDGLGLRTF
                     HGRLLSSAIDSGVCVQPVAIRYLRGGKPCSVAPFIGDDDMLSHLLRLLDSELSEVQIQ
                     LLTPIASSDLNRNALARAAHQAISHALFGAAAAPATARTETESQAA"
     gene            complement(34191..34775)
                     /locus_tag="VFF22_00225"
     CDS             complement(34191..34775)
                     /locus_tag="VFF22_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HZX15281.1"
                     /translation="MNYLAHLHLGGDAPAQLLGSLYGDFVKGPLAGQWPADIEAAIRL
                     HRRIDVFTDSHPLQLQARARFAVERRRYSGILLDVFFDHCLALHWADYAAEPLPRFTG
                     RVYQVLAAEPRLPERLALIAPRMAAQDWLGSYREFAVLEQVVAGIDRRLSRPGLLIGG
                     LGELERLYQPLLEDFRQFYPELQAYVARERGLAG"
     gene            34902..35204
                     /locus_tag="VFF22_00230"
     CDS             34902..35204
                     /locus_tag="VFF22_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010220843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZX15282.1"
                     /translation="MQLDIDEVIKALAHPVRRDILHWLKDPQAYFADQDHPLEIGVCA
                     GKIDQRTGLSQSTVSAHLATLQRAGLVSSKKVGQWNFFKRNEETIQAFLNHISQAL"
     gene            35243..36409
                     /locus_tag="VFF22_00235"
     CDS             35243..36409
                     /locus_tag="VFF22_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX15283.1"
                     /translation="MPAALLVLALSAFAIGTTEFVIMGLLPEVASDLGVSIPGAGWLV
                     TGYALGVAIGAPFMALATASWPRKASLLTLMSIFIAGNLFCALASDYQMLMLARVITA
                     LCHGAFFGISSVVAASLVPANRRASAVALMFTGLTLANVLGVPLGTALGQYAGWRSTF
                     WAVTLIGVIALIGLWHVLPSKTDEAKADFASEVRALRGAGLWLALSTTVLFSAAMFAL
                     FTYIAPLLGEVTGVSPNGVTWTLLLIGLGLTLGNVLGGRLADRSLKATLIGVFVAMAV
                     ISTLFSWTSQALIPAEITLFLWATAAFAAVPALQVNVVAFGSGAPNLVSTLNIGAFNL
                     GNALGAWVGGLVIAEGFGLTRVPLAAAALAVLAMLATLLTFSTRSPEPQLALNE"
     gene            36427..37476
                     /locus_tag="VFF22_00240"
     CDS             36427..37476
                     /locus_tag="VFF22_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HZX15284.1"
                     /translation="MTTLFDPIQIGDLQLNNRIIMAPLTRCRADEGRVPNALMAEYYV
                     QRASAGLIISEATSVTPMGVGYPDTPGIWSNDQVRGWSNITKAVHAAGGKIVLQLWHV
                     GRISDPLYLNGQLPVAPSAIQPAGHVSLVRPIKDFVTPRALETEEVADIVEAYRLGAE
                     NAKAAGFDGVEIHGANGYLLDQFLQSSTNQRTDRYGGNLENRARLLLEVTDAAIEVWG
                     AGRVGVHLSPRADLHDMGDANHAETFTYVARELGKRGIAFICAREQEADDSLSPSLKE
                     AFGGVFIANERFTKDQANAWLAEGKADAVAFGVPFIANPDLPARLAQDAELNAPHPET
                     FYSQGAVGYLDYPTL"
     gene            37812..39086
                     /gene="codB"
                     /locus_tag="VFF22_00245"
     CDS             37812..39086
                     /gene="codB"
                     /locus_tag="VFF22_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HZX15285.1"
                     /translation="MNQAQQDSDYPLSEVPSDARKGLLSTSILLFGFTFFTATMFAGG
                     KIGMAFDFTTLLWAAVIGNLLLGLYAAALGLIACRSGLNSVLMGRFCFGEIGSKLSDF
                     LLGFTQIGWYAWGTATIAIVLVRLLDLPEGLTIPLMVLFGFGFCVTAFIGYRGLDLLS
                     RVAVPAMLILLVCSLWIATRDAGGWAALLAIEPKDDMTLSMAITLVFGTFVSGATQAT
                     NWTRFAKSGRVAVLSSLIGFFIGNGLMIIAGAYGAIVYQQPDVVEVLVLQGLSMAAVV
                     MLFLNLWTTQDNTIYNFAAAGCNMLRSKRRKTVTLVGAGIGTLLAIAGMYEMLIPFLI
                     LLGSIIPPIGGVIMANYFYCHKQNYPKLVDAQLPAFNWAGLGAYLIGALCAYLSPWVA
                     PIVGILVGAAAHIVLFEVQRVFSGQLQAKANP"
     gene            39083..>40287
                     /locus_tag="VFF22_00250"
     CDS             39083..>40287
                     /locus_tag="VFF22_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017847007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HZX15286.1"
                     /translation="MSLRVADVLALPGLQSLQLRAGAAGLDNPVRWPYVAENAGIADW
                     VMGGELIFVTGINHPRDEANLLQLVHEGHQQLVAGLVILTGEEFIQSIPSSVINEAER
                     LGIPLLEQPYQLKMVIVTQAIGTALVEEQVLGRSRLHVLEQLLEGDYRSLDVLLQRAH
                     NLRLPLAVPRQVALFKLEGGERLFTAEQPEQAERQLQANRQQLQRQLEQSLGTLGDAL
                     APICQGEHWIALLPAHDSPHEQRNRRAMMRLLEQLNQQLAPQRLFLGLSDANHHPARY
                     AHGLSEARQALVAAQSFPERLGLCCFNELGVIELLGAIRDRTLLDRFVDNVLGALIGD
                     DSRHEAVLIPTLEAWFHENGNLALAAQRLAVHRNTLGHRLQRIEALSGCSFDDPHDRL
                     NIAVALQIWR"
BASE COUNT         8319 a        12729 c        11632 g         7607 t
ORIGIN      
        1 gctggcagta ttgtcctgcg gccatactca gcacctgcgc caccagccgc cctggcagaa
       61 tcgcgcgtgg gtgctggatg ctcatcaacg ccaggcgcac gtgggaggac ctttcctctg
      121 cggctggtgc gcggctgaaa aaggcgcaag cgaggcgctg acagaacgcc cgaaccaaga
      181 tttgaacaag gagcggtaaa tgcccgcacg caacattctg gtgatcaact gcggcagttc
      241 atcgatcaag tttgccctga tcaacgaagc gcaagacgcc tttatcctca gcggcctggc
      301 cgagcgcctg ggcagcccgg atgcgcaagt tcactggcaa cagggcaaca gcaaagacag
      361 cctgattctg ccccatgccg atcaccgcgc cgccctcgcc caggtgttac ctctggtgca
      421 ggccgccgct ggtggccaac tgcacggtat tggtcaccgt gtggtgcatg gcggtgagca
      481 cttcactgcg gcctgccgcc tggatgcgct gagcctcgcc gcaattcgcg ccaccgcgcc
      541 gctggctcca ctgcacaacc cggccaacct gctgggtatc gaagcggcga tcaagctatt
      601 ccccagcctg actcaagtag cggtgttcga tactgcgttt caccagaccc tgcccgaaca
      661 cgcctaccgc tacgccctgc ccgaccacct gtaccgtgag cacggtgtgc gccgctatgg
      721 ctttcacggc accagccacc gttttgtcag ccagcgcgcc gcagaaatgg ccgggctgcc
      781 ggtcaatgcc agcagctggc tggtggccca cctgggcaat ggctgctcga cctgcgccat
      841 cgtcgatgga caaagccgcg acaccagcat gggcctgacg ccactggaag gcgtggtgat
      901 gggcacgcgc agcggcgatg tcgaccccaa tctgcacagc cacctgtcgc gcaccctggg
      961 ctggagcctg gaacagatcg acgccatgct taataaagac agcggtctgc tgggcctgtc
     1021 cggcctgtcc aatgacatgc gcaccctgga gcaggcccgc gaacagggcc atgccggcgc
     1081 caccctggcc atcgaggtgt tctgctaccg tctggccaaa tccctggcag ccatgagctg
     1141 cgccctgccg caactcgacg gcctgatctt caccggcggc atcggcgaga actcggcgct
     1201 gatccgcagc aagaccgtca ctcacctgaa attgctcaac ctggccctcg acgagccggc
     1261 caacgcccgc accgtgcgcg gctcagccgg gccgatccag gccgatgggc acccgcgtgt
     1321 gctggtcgta ccgaccaatg aagaacgaca gattgccctc gacaccctgg cgctactcga
     1381 agcctgatcg tcacctgact actacggagc gcgaatgcac accttcttta ttgcccccac
     1441 cggtttcggt gtcggcctta cctcgatcag cctgggtctg gtcggcggcc tggagcgtgc
     1501 tggcctcaag gtcggctttt tcaaacccat cgcccagccg catcagggtg acatgggccc
     1561 ggagcgttcc agcgagctga tcgctcgcac ccatggcctg cactcaccca agccgctggc
     1621 cctggcgcat gtcgagcgca tgctcggcga cggccagttg gatgaactgc tggaagaaat
     1681 catcagcctc tatcagcaag cggctgcagg caaagacgtg gtgatcgtcg aaggcatggt
     1741 accgacgcgc caggccagct acgccgcgcg ggtcaacttc cacctggcca agagcgtcga
     1801 tgccgaggta attctggtct ccgcaccgga acaggaaagc ctcagcgaac tgagcgaccg
     1861 ggtggagatc caggcccagc tgttcggcgg gccgaaggac gaaaaagtac tcggtgtgat
     1921 cctcaacaag gtgcgcagta acgatggcat cgaagccttc gccactcgcc tgaaagaact
     1981 ctcgccgctg ctcaagggcg atgacttccg cctactaggc tgcatcccct ggcaagacga
     2041 gctgaacgct ccgcgcaccc gcgatattgc cgacctgctc ggcgcgcggg tgctgaatgc
     2101 cggcgattac gagcagcggc gcatgctgaa gatagtgctc tgcgcccgcg cggtggccaa
     2161 taccgtgcaa ctgctcaagc ccggcacatt ggtggtgacg cctggggatc gcgacgacat
     2221 tatcctcgcc actagcctcg ccgccatgaa tggcatgccg ctggccggcc tgctgctgtg
     2281 cagcgacttt gcccccgatc cacggatcat ggagctgtgt cgtggcgcgc tgcagggcgg
     2341 cttgccggtg atgacggtaa gcaccggctc ctacgacacc gcgaccaatc tcaaccgcct
     2401 aaataaggaa atcccggtcg acgacaagga gcgtgcggaa aaggtcgccg acttcgtcgc
     2461 cagccacatc gaccacgaat ggctgttcgc ccgctgcggc acgccgcgcg agctgcgcat
     2521 gtcgcctccg gcgttccgct atcagctggt gcaacgcgcc aaggcagcca acaaacgcat
     2581 cgttctgccg gaaggcagcg agccgcgcac cgtgcaggcc gccgccattt gccaggcccg
     2641 cggcatcgct cgctgcgtgc tgctggccaa gccggaagac gttcacgcgg tggcccgcgc
     2701 ccacggcatc gaactgccgg aaggcctgga aatcctcgac ccggacctga tccgcgagcg
     2761 ctatgtcgcg cccatggtcg agctgcgcaa gggcaaaggt ctgaacgcgc cgatggccgc
     2821 cgcgcagctg gaagacaccg tggtgttggg caccatgatg ctcgccctgg atgaagtcga
     2881 cggcctggtt tccggggcga tcaacaccac cgccaacacc atccgcccgg cgctgcagct
     2941 gatcaagact gccccgggct acaagctggt gtcgtcggtg ttctttatgt tgctgccgga
     3001 ccaggtgctg gtctatggcg actgcgcggt aaacccagac ccgacagcgg tggaactggc
     3061 ggaaatcgcc atacaaagtg cggcctcggc cagcgctttc ggcatcatcc cgcgggtggc
     3121 gatgctcagc tactccactg gcgactccgg cagtggcgaa gaggtcgaga aagttcgcga
     3181 ggccacccgc ctggccaagg ctctggaccc gcagctgctg ctcgacggcc cgctgcaata
     3241 cgatgcggca gcaatagaaa gcgttggccg gcagaaagcc cctggcagcc cggtcgccgg
     3301 ccgcgccacg gtgtttatct tccccgacct gaataccggc aacaccacct acaaggcagt
     3361 acaacgcagc gccgattgcg tcagtgtcgg gcccatgctg cagggcctgc gcaagccggt
     3421 aaacgacctg tcgcgcggcg cgctggtcga tgacatcgtc ttcaccattg ccctgactgc
     3481 gattcaggcc gccaacctgc cgcagtaacg cacagccagg gctgcccagc tgcggcccta
     3541 gccaagcaaa agcgctaaaa atccgcgatc cgcagcgatc accagcgttt cgaaaggctt
     3601 gcagttcggt acacagacgt taacctgtgc gccgaccgat ctgtggtgga aaaccacagg
     3661 catagataag tcggggccta cgcccctttt tggaggcatc tgtccccatg ctgcattttc
     3721 tgcctgctcc attacgcggt ctgcttgccg gcctgctact cgccctcaac acccttatct
     3781 gctgctggcc gctatttatc ggagccctgc tgaaatttct gctgcccatt ccgaaggtgc
     3841 agcgcgcgct gcgcgcggca atgcatgcga ctgccgagtt ctggatttcc gtcaacaaat
     3901 tttggatgca tttggtaggc aagaccgaat ggaatgtcga aggactcaag ggtttcgaca
     3961 tgcagcactc ctacatggtg accagtaacc accaaagctg ggtcgatatc ctggtactgc
     4021 aataccagct caaccggcgc atcccatttc tcaagttctt cctcaagcag gaactgatct
     4081 gggtaccggt gattggcctg tgctggtggg cgctggaatt cccctttatg aagcgcttca
     4141 gcaaggaata cctggccaag cacccggaaa aacgcggcca ggacctggcc accacgcgca
     4201 aggcctgcgt gcgttacaag agcaatccgg tgtcggtgtt caatttcttg gaaggcacgc
     4261 gcctgtcgcc caccaagcac gcgcagcagc aatcaccgtt caagcacctg ctcaaaccca
     4321 aggccggcgg cattgccttt gtgctggatg ccatgggtga gcaattgcat agcctggtca
     4381 acgtcaccat ccattacccg cagggcaacc cagggttctg ggacctgctg tgcgggcggg
     4441 ttgaacaagt ggtggtacgc ttcgatcaac tgcagatccc cgaggaattt attggcagca
     4501 actacgatca ggacgaggcc taccgcctgc agttccagca gtgggtcaac cagctgtggc
     4561 tggacaagga tgcgcagctc gacgagctga agaagcgcta tcccgcgcgc taaaccgcta
     4621 ccgcagcagc ctccaagccc gcttaaccaa cgggtttttc attgcgtaat ggcaagttgt
     4681 gccaatccag ctgcgcgggg aaagtcttga tctgctcggt gctcagcccc ggcgcgacca
     4741 gaaagccctg catgattgcg cagttgtgcg ccgccaacca atcgcgctga gccatggttt
     4801 ccacgccctc ggcgatcact tccagcccca ggttacggcc caggtcgatg atggtgctga
     4861 ccaccgcagc gtcacgcgga gagtcgagca tattagagat aaatagccgg tcgatcttca
     4921 gcgtatccag ctcgaaatgg cgcaggtagg ccagcgagga gtagccggtg ccaaagtcat
     4981 caatggcgat cttcacaccc agctggcgca actgacgcag ctgttcctga ctggtttcca
     5041 ggtcttgcat cagcgcgctt tcggtgactt ccacttccag ttgccgaggg ttcaactggt
     5101 gctcttcaag cacccggcgc aagtcttcga ccagctgcgg cataccgaac tgcaccggac
     5161 tgacattcag gctgagcacc agctcgttgc cgtactgctg gctcagctgc tggcatttct
     5221 cgatcccggt ctggaaaatc cagctgccca ggcggttgat caggcgagtc tcttccagca
     5281 gcggaataaa cacccccggc gccacggtgc cggcaacccg gtgctgccag cgcagcaagg
     5341 cctcgaagcc gcgcaggcgc ccgctatcca gatagatctg cggctggtag accagtacaa
     5401 agtcattatt ctcgattgca ctgcgcagac tttcctcaag catcaaccgt gagcgcgcgc
     5461 ggccgttcat ttccggggag aagaagcggt attgctggcg gcccgtgcgc ttggcctcgt
     5521 acatggccat gtccgcggcg cgcaacaagc cttctacggt ttgcccacat tcggggaagc
     5581 aggcaatccc tacgctcgcc cccagggtca cctcaatgcc gtcgaggtta tgccgcaccg
     5641 acacctgctc gatcagtttc tcggcgatgc gcgccgcgtc ttccgggtgt tccagagtgt
     5701 cgagcagggc ggtgaattca tcgccgccaa tccgcgccag gctgtcatag gggcgcaggc
     5761 tggcctttag ctgttcaccg accagcttga gcaagcggtc gccgacatcg tgaccaagcg
     5821 aatcattgat ccgcttgaag ccatccagat cgagatagag caccgccata cgctgaccgc
     5881 tgcgattgat ccgcgacagc gccgactcca gggcctgata aaagccacgg cggttgagca
     5941 ggccagtcag ggcgtcggta atcgcctgcg actccagttg ctggtgcaaa cggcgcacca
     6001 ccgacatatc cagtgccatc accaccatgg cgtgctgcag gcgcggcaag ggtgaacagg
     6061 acagcgccac cggcacctgc ccgccgttca acgtgcgtag gctggcatcg tgcaagcgat
     6121 aagcctcgcc gcgccgccac tgcaggtaaa actcggactg acgccaatcg ctggttactt
     6181 gcgggctgac caggtaggac agcatttcac tgccttccag atcgttgatc gaaccttcaa
     6241 gcatgcgcgc catggccggg ttggcgaaat tgatgcggcc atcttcgccc accaccatga
     6301 tgccttcggc ggcgttatcc agcaccgaag cattgaaggc ccgcgccgtg tccagttgct
     6361 gagtcagttg cagcagttcg ctgcgactgc gctcatgttc aagcagggca tgaatcttat
     6421 ggcgtagcac gttggagtcg aacggtttga gcataaagtc caccgcgccg gtggcgtagc
     6481 ccttgatcac cgagtcctgg gtctgggaga tggcggacac gaagatgatc ggggtgtaac
     6541 gggttcgtgg gttgccgcgc atcaatcggg caacttcgaa gccgtccatg tgcggcattt
     6601 gtacatccag cagcaccagg ccgacgtcct cttcgagcag acactgcagg gcagcttcgc
     6661 ccgagtcgac acagcgcagt tgccagtcgc cctcttccag cagggcttgc atggcctcca
     6721 gattttccgg acggtcgtcc accaccagca gaatctgcgt actggtggca accttcagct
     6781 gcacgtgtgc catatgttct ccctgtatcc ctggcgagcg ccttgcgact cctgttgcgg
     6841 ttcaggttag caggcgctgg caaaactact gcggcggtac tgcaggtact tcaaatgaac
     6901 gcctcgctgg ctcggcagtg gtcagccagc gcaccagcag ctcgagcaag gcctcgcggc
     6961 taaccggttt ggccaggtag tcatcggcgc ctgcggtgat gcacttctcc cggtcacctt
     7021 tcatcgcgtg ggcagtcagc gcgatgatcg gaatgctgca gccgtgctcg gtcttgagca
     7081 gccgggtggc ggtgtagccg tccatggtcg gcatggccat gtccatcagg atcagatcga
     7141 acggctcgcg ctcgtaacag ttgatggcct cctggccgtc cttggccgaa ctgacgatca
     7201 gccccacctc gtcaagcaga gcggtcaggg catacacgtt gcgcacatca tcatccacca
     7261 gcagcacgcg ccggccttcc aacggattct cgattctcgg agcacctgcc cgcgagggat
     7321 cgcgcacgct gccaaggaag ccctgcaccg cctcgctcag ctcctgcatg tcctcgccgt
     7381 gtttgtgcac caccaccgcg ctgtaacggc gcaggcgctg cagggcctgg cgggtgacat
     7441 cgacaccggt attgattacc acccgcgtgc cattcagcgg gcgctggcgg ttgagggttt
     7501 ccagcagctc aaaaccgtcc tgatcgggca gatcaaggtc gatcaccaat gcattgaaca
     7561 tcccggaggc ataagcctgc tgcgcctcat cggcgcgcgc gcaggcatgc acatcaaagc
     7621 ccagttgttg cagatgctca cggtaatgtt cgcgctcaac ctcaacatcc tcgaccagca
     7681 acaggcgctg cgccggcgat tggtcgagct gttgcagatc gacaaatacc tgctccagat
     7741 ccgcacgctg aatcggttta accagatagc gcgtaccgtc atcgttccag tccaccggtt
     7801 gtggtacgca ggagatgata tgcaccgggg tgctgcgatg gctggcatgg ctgcgcaggc
     7861 gacggaacag ctgccagccg ctgatatccg gcagcagaat atccaggatc accgcgctga
     7921 agcgctcgct ctgcagcagg ttgagcgcct gcaccccaga gcggcaatgc acgctggcaa
     7981 agccgtgggc atgcgcttcc tcggctaata ccacggcaaa attcacatcg tcttcaacaa
     8041 tcagtaccgc agggccttca ccgctgcgct gcggcgcatc gacttctggc tctgcctggg
     8101 cttgcgccac cacccgcacc ggcagctgca cggtaaagca ggagccttca cccaacgcac
     8161 tttgcaggct gatctcgccg tccatcgcca gcaccagctg tcgggtaatc gctagaccca
     8221 gcccggtacc accgaaacgc cgactggtag agccgtcgat ctgctggaaa gcctggaaaa
     8281 tctgctcatg ctgctcggcg ggaataccaa taccgctgtc gcgcacgctg aagttgagca
     8341 gctcatgtcc ctcctccggc tcgccgctgg cgcaggacac ggtcaggctg acctcgccgt
     8401 gctcggtgaa tttcagcgcg ttggacagca ggttgcgcag gatctgctgc aggcggatgc
     8461 ggtctgtgtg aataaccctc ggcaccccgg cctccaactg ggtatgcaga cgcaggccct
     8521 tgatctcggc catcggccgc aggctggcat ccagctcgac cagaacatcc tgcatattca
     8581 gcggttcaag cttgagctgc atgcgcccgg actcgacctt ggccagatcc agcacatcgt
     8641 tgatcaactg cagcaggtcg ctgccggcgc ggtggacgat atcggcatgc ttggcctgct
     8701 tctcggtcag gttgccggcg ctgttcaggc gcaactggtc gctgagaatc aggatgctgt
     8761 tgagcggcgt gcgcagctca tgggacatat tggcgagaaa ttcagacttg tagcggttgg
     8821 ccagaatcag ttgctcggcc tggtcgcgca ggcgctgctc ggcggcctta cgctcgctga
     8881 tgtcgataat caccgcctgc accagcgcgc cctcgccacc actgatcggc gacagcccca
     8941 cttccagggg aatctgccga ccatcgcggt gctggccgaa cagctcgcgg ttattgccca
     9001 tgcgccgcgg ttctggactc ttctgatagc cctgacgcat cggtacatgg ctgccacgca
     9061 gggccgtggg cagcagaatt tctaccggtt gggagagcaa cgcctcacgg ccataaccaa
     9121 acatctgctc ggtctggcga ttgaccatgg caatacagcc tttgttgtcg accagcacga
     9181 tggcattggg tgacgcctcg accaccaggc gaaagcgctc ttcggcgccc ttgcgcgcgc
     9241 gcaggtcaat caggtttatc aggtaattga ccccaccaga atcgcccagc acgctcaggc
     9301 tcagcgccac cggaacttct tcgccgaggt cgcgacgcgc caggtattcg ttggacagtt
     9361 cggaggtgtc gtgatcgatg gccagggcgc tgagttgctc aacgctcggc agaaagcgtt
     9421 tgacccgctc gccgaccaag ccatcgggcg ttgagtcgag caagtcggca gcgctgcggt
     9481 tggccatctc gatgcggccc tgggcgttgc acagcaacgt cgccaccggc agccgttcga
     9541 ccagcaggcg gaagcgctcc tcgcgctcac gtagcttgtc ggtagccagg tggttggcgt
     9601 gcagctcgcg ctcgcgcaaa tacagatagc cgccgaccag caaggccagc aaggcggctg
     9661 ccaccaagcc gatccacaag ctgataccac gactggaaaa agccgcctca taggctggcg
     9721 tactggttac ccgcagcagc cagttgcgcc catacaaggg taattccagt tcatgctgaa
     9781 agcgtggctg ctgggcgaca ccctgtatct cggcgcttaa caacggggtt tgcagcgcct
     9841 gctgatcctt gatcaccaca tcgaagcgct ggctctgcgc accaagaatg ccttccatca
     9901 gctcatggct gcgaaacgct ccatgcacca cgccgagcaa ggctgcgcga cgttcttcgg
     9961 cagtgctctg tggcatgcca ggctgatata ccgacaggta cagcagtatg cccccctgca
    10021 cattgacctc tgtttcctga cgcagagtga ccggtgcact gagaatcgcc tgccccgtgt
    10081 cgatggcctg ttcgactgcc gtacgacgca cggcttcact gagcatgtcg taacccagca
    10141 cccgccgatt gcgccaatcc aatgggctga tgtagtcgat caacagatag tgctgacgcg
    10201 cccccgcagg gaacagccgg tagtccgcac gcccctggct ctgctgttca agcaaataag
    10261 catcgagctg ttcggcgggc agataacgcg accacgccag ggccaggatg cccggataac
    10321 gatcctgcaa ctgcaactga tcaagagcgc gctcccactc ggtcagggac acatgatcac
    10381 tgccggtcat caacccggaa acgccgcgca ggaccatttc ataggcttgc atgcggctaa
    10441 tcacccgctg accgatgtcc tgcgcttcca gctcgaagcg ctgctgattc tgctcgagct
    10501 cacgctgctc gaagccctgc cactgccaga cgatcaatgt tcccaggcca atcaacacag
    10561 ccaaggtcac ccacaacggc aaccagggtt tacccgaagt ggaagcaggc ctgtcgtcca
    10621 tgtactctcc tttttcgtcc atgttcggcg cgtgcagcgc acgatggggc aggattattg
    10681 gaaccactgc cagcagtcta gtcacgattt gaatgggggg ccgcgtctaa attacaggca
    10741 taaaaaagcc gcgcattcgg ccaatgcgcg gcttttttca gcctgcggtt acagcggacg
    10801 caggttaatt tctacccgac ggttctgcgc gcggccggct tcactggcat tgctggcaat
    10861 cggttggttc gggcccgcgc cataagacga cagccgcgaa gccggaacgc cattggcgct
    10921 caggtaagag gcaacacttt gcgcacggcg attggagagg ttctggttca actctagcga
    10981 gccggtgctg tcggtatggc caacgatatc aatgccgttc ttgttgaact ccttgaatac
    11041 cgtgaccagg gaattgaggg ttggataaaa gctgctggaa atatccgccg agttgctggc
    11101 gaaggtgata ttgcccggca tgatcagctt caggtcatca ccattgcgct gcacctgcac
    11161 gccagtgcct tgcagagtct ggcgcagctt ggcttcctgg gtgtcgacgt agtagccgta
    11221 accaccaccg gccgcaccac caactgccgc gccgatcagg gcacctttag cacgatcctt
    11281 cttgcttgat gtggccgcac caatcaccgc accagtgaca gcgccgacac cgccatagat
    11341 gccagccttg ccggcctcac gctcaccggt ataagggttg gtggtacagc cggcgagtat
    11401 gccaagaccg agagttacga cagccagatt tcgccacagc ttcatgcgta cttcctttga
    11461 attcttatcg ggacaggttt gcacctgagt tttcgagcct gaaacgcggt ttaagttcct
    11521 ctcgccgcac aattgagtgg aagggctatg cccgaataaa ggggttgtcg cgcatctcat
    11581 caccgaggcg cgtatcagcg ccatgaccag tcaccacgat ggcatcctcg tccagggtgt
    11641 ataaacgctg cttgatcgag cgctcaatgg tcgggtaatc accgccccac aggtccgtgc
    11701 ggcctatgcc acgcttgaac agggtgtcgc cggcaatcaa cagcttgtcc tggggaaacc
    11761 agaagctcat cgaacccggg gtatgccctg gcgtatgcag tgccacgccg caaccgcagg
    11821 ccagctcttc gtcatcactc aaccattgat ccggggccgg cacaggggtg tagggcacgc
    11881 caaacatgcg gcactgcatt tccagattat cccagaggaa ctgatcctcc ttatgcaggt
    11941 gcagggtcgc gccggtcttc tccttcatct gcccggaggc aaggaagtga tccagatggg
    12001 cgtgagtgtg gatgatgctc accaccttca ggccatgggc ctccaagcgc gccatgatca
    12061 gctcagggtt gccacccgga tcgacgacga tggccttctt ggttaccggg tcaccgatga
    12121 tcgtgcagtt gcaatgcaaa ggcccgacgg gaaaggtttc gcggatcagg gtcggggttg
    12181 cggcagaact catggttatt cctcagcgcg ataatcgctg ggcagtttac ccgtccactt
    12241 cttgaacgca cggcgaaaat tcgatgggtc gctaaagccc agcaggtagg cgatttcata
    12301 cagcggcagg tgggtggtgg tgaggtactg caaggccagg cttttacgca cttcatcgag
    12361 cacttgctgg tagctggtgc ccatggtcga caggtgccga cgcagactgc ggccactggt
    12421 gtgcagcgcc tcggctgcgc tggcaaggct ggggaagtcg ccagggcggg tcagcagtaa
    12481 acggcgtaca cgggtcaaca gaccgtcctg cacatccaga ctcaccaata gggcttcaca
    12541 ttgttgctcg cacatctgca ccgtagccgg gttggccagg gccattggcc ggtcgagata
    12601 ggtgattggc atgctcaggc ggtgataagg ctgatcaaat accactggca caccaaacag
    12661 ccgctcgtaa cgctcagcgt agtgcggcgc ggcatgggca aagcccacgt ctacgtcctc
    12721 taaacgctcg cccaccagaa atcgcacaat agtgaagcaa ctggtcagta aaccttcggc
    12781 ggcgaaacga cttagcggac ccagcggcat ggtttcactg gcgcgcagtt caatccgcgt
    12841 acccaactcg accatttcca gctcgtaggt aagacccaag atccggtagt acttgagggc
    12901 gaactggata gccttaccca ggttggcact ggacaacacc gcatagccga ggatgccgtg
    12961 ggtcgaaaca ttcagccgct gcccaagcag caaacccaga gcaggctcat cgcacagacg
    13021 caaagcgctg ctggaaagtt gagtgaaatc aataaaggac aaccgtccct gcggattacc
    13081 cagcacttcg gggcgcaccc gtgcctcggc aaacagctgc gcgcgcggca cgcccaactc
    13141 ctcgaccaga tcaagtaagg cagcggcata agcaacggga accagctcag cggaaaaact
    13201 caatgatggc ttcatgggct tcttattatc tggccagata tgaccactga ctgcgacgat
    13261 aaccttgttc gacaaaaccc gacaagggca tccctgcacc acggccaggt caatcagcct
    13321 gtgcagcttg tgcgagggtt ttcaccatac tcaggacttg agcggccgat caccacaacc
    13381 gaaggttggg cgcatgcatg gcgagggcgt cttccacgca caaacaagtg ctcaagcacg
    13441 agcgtttgat aacccaacca actaagctga caagccatta aaattggtac actcgtgcaa
    13501 tattggccgt atatgacctt aatattggct gttgtgaacc tttgccgaag tgtttggcga
    13561 atccatagtg agaccacgct tcgcttcaat cacagactca gggaatcagc atggtcagca
    13621 ctactccgga aggtttgcag attcgtccac ggcatatgga tttcgacttt cccaatcctc
    13681 tgccgcgcca ttggcacagc ggtgatgcct tcaaaagtca tctattcgat gccatgtcgg
    13741 tgctgttccc cgatggcgag cgcttcttta tcgattcggt gcggcacttc cgcgaccaga
    13801 tcgacgaccc ggtgttgaaa gagcagattc gtggttttat cggccaggaa ggccaccaca
    13861 gccgcgaaca cttggaatac agcaaccgcc tgcgcgacct ggggtacaac atcacccgca
    13921 ttgaaaagcg cgcacgcgca cgcatccgct tcgcccagaa gaaactctca ccacaacggc
    13981 aactggctgc aactgctgcg ctggagcaca ttacggcgat catggccgac ggcctgctca
    14041 agaacccgaa atacctggca ggcgctcacc caaccatggt gcgcctgtgg cgctggcacg
    14101 cgctggagga aaccgagcac aaggcggtgg ccttcgacgt gtacaaccag gtatgtggca
    14161 gccgcaaact gctacgcaga gcgatgatca tggccagctt cttatttgtg ctcgatacca
    14221 ctcgcggcct ggcgcatatg ctcaagcagg atggcctgct gtggaactgg cgcgtgtggc
    14281 gtgatggcat ccagtggatg tggggcaaag aaggcgtgtt ccgtccgctg atcggcacct
    14341 accgcgactt cttcaagtcc aacttccacc cctgggagca caacaacctg gagctgctct
    14401 accagacccg cagcgagttc gatctgcctg cgcaaagcca ggctgcttaa gcctggcgat
    14461 gagccgaaac gacaagggag ccatcggctc ccttttttat gcacatctac ttaaccgata
    14521 gcgacgactg ctaacgccgc tatcggactc tttcagccct gcagaaaacg caacgcatcg
    14581 gcagcacttt gctgcgggat ttcttccata gggatgtgcc cgacgcctgg gtaggttttt
    14641 acctggatac ccggaagatc acgctgccac agcggtacat gcttagggga aatccagcga
    14701 tcacgctcgc cccacaacag cagagtgggc gcctgaatct gtgcgacccg agccgcagta
    14761 ccgtatagct cttctttatt gaccttgagc agcacgcgga agatatccat catcgcccgg
    14821 cgattacccg gccgccgcgt caggtcgttg tagcgatcca ccacacccgg cttgatgcgc
    14881 gccggctcac cgtagacttc cttgatgccc tgggcgatca gcgcacgcgg catccacagc
    14941 ggcatggcca cggtggcacc gggcaacgcg gcggcggcaa tcatccacgg caccttgtgc
    15001 atgtgataac cggcagggtc gatcagcacc agtttctcca ctcgctgcgg ctgcgccaag
    15061 gcgaagttcc aggcgatata accgcccagg gagttgccga caatcgacgc tttgttcacg
    15121 cctagatagt cgagcagcag gccaaacacc ttgatcattc gctcggcgga atacacgcca
    15181 tcgcgagccg ggccggtaag gccaaaacct ggcacatcga aacggatcac ccggtaatcg
    15241 gcagcaaaag ccggaagcca gtcgtcccag gtgtgcagcg aggccaccac accgtgaatc
    15301 agcaccagca ctggtttgtc gcgactgccc tcatcccggt aatgcaggtt gaagccgtcg
    15361 agttcgacgt agcgcgagcc cttggtggca ttgccatagc gcgccttgag acgctccagc
    15421 ggcaaggcac cgaaacccag gcgggcaaaa ccgtgcgcca gcgggggcat cagcggaaga
    15481 ctggaagcag acatgcaggc acctctagat tgttgttatg gggaagacgt atccgcccta
    15541 tcagatcacg cccacttcaa cctgacgcct aacctaggtt gagctggagg ccgggcacaa
    15601 cgaggcgatc agcagttcgc tgagcacacg cttggcctgt tcgatttcct cgcggctggc
    15661 tgcgcgaccg agcaggtcca aggaggccgc ttccatggcc cagatcagcg ctcgataggt
    15721 cagagggtcg gtcgtatggg tcgcaccatg cagacgatcg gcaagcaatc gcgccatctc
    15781 ggtatgagcc cgctgccggt gcgcggccag cggggagtcg gtgcgcaggg cttcttcctg
    15841 catcagacga atgatcgggc cgacagccag gtgatagtcg aagtaacgcc cgaccatacc
    15901 ttgcaaccag gcctgcgggc tgccggcgag agtctgcatt tcactgaagc gcagcagcat
    15961 caaggagaca gccgtctgat agataccttc gagcacctgc attttgtttt cgaagtattt
    16021 gtagaaggtg cgacgcgaca cattggccgc cgccagcaaa tcattcaccg tagtttcggc
    16081 aaggccctta cgagcaaata ccggaatcgc ggccagttga atattggtac gctgcagatg
    16141 gccgataagc ggcccgtcac cgtcctgctc ctgctgctgg gccaccgcgc cgtagccgcc
    16201 gaggtcttca aacaccgcac gcacagcacc gagttctgcc aaggtttgct gcattgcact
    16261 tttcccaacc aaggcgcggg tgacgcgagc cgccaagctg gttacgctga gccacgccac
    16321 cgccgcagga ttattgttgt gctgaacacg ctaacacgcc gcctttgcct accgctggcc
    16381 atccttttgc tggttctgct gtcacccgtg caggccgccg agctgttcta tctcgggcag
    16441 aagattccgg acatccacaa aacctggaac agcgctgact atcagcaact gctagaggca
    16501 cttaacaagg tcgacagcac ccagctcaac gccctgcccc gacgcagcgg cgagttcacc
    16561 gggccgatct atgagcgcat ggttagtgag gagaacttcc gcccgcagct gaatatctac
    16621 gcgcccctgg agctgcggca gaacgaagcc cgcgaagcgt tgtttcgcct caaggaactg
    16681 atgcgtctgt attttgattt tcgtgccgtg caacagccct atgccgccga ggcgctgggg
    16741 ctgatgagtt attcactgcg ccagcaggcg gtgctgttca ccttgaccac cgaattctgg
    16801 atgaccctct cggaaagcga gcaaagcaat ccggtgcgac tgcaaggcct gcatgaaacc
    16861 aaagcagcag cggccatgct cacttccagt gccttcgact acctcggcct gaccaaacaa
    16921 ttcggccgtg acgagctggt gctctacagc gccgagctaa gcaagcagct gcccgagctg
    16981 tttatccatt taagcgcaga tgtgcgcgaa accttgctgc agcgcgtcga gcaactggcg
    17041 cagcagcacg cctatgcaga agtacagcaa aacatgcgtg agctacagcc ggtgctgctg
    17101 agcatccagc aggatgtgca ggccaaactg gcggcggccg cggcgcgacc gcgcaaggcg
    17161 cctacgcgca gcctcgacct gtcggtacca gaagagtcag cgcagtagac cgaggctttt
    17221 ggcccgtgcc acggcctgag tgcgccgttc gacgcccagc ttgctattga tatgccgggc
    17281 gtgagtctta acggtgtgca atgagataaa caagctgtcg ctgatctcct ggttggagca
    17341 gccttgggcg atcagccgca gcaccgacag ctcgcgcgaa ctgagggcgt cgaccactcc
    17401 tgcatagtca tcctggctga atgcctgcac tgactgcggc aggtgctgca acagggcctc
    17461 aaccaagggg ctaacctcat gcgtcagcaa ctgctcacgc aaccattcgg gctgtcgtac
    17521 cagcaggcca tgaaagggta ataaaccgcc accggccgcc gcctgcaacg cgctgcgcaa
    17581 ttgctgctgc gcatcacgct cgcgcccacc gtcacgcagc agggccgtca gctgcatctg
    17641 cgcaatcgcc cccagcaact gcccgccggc gttctgcgcc agttgactca gcccgcgcaa
    17701 acgccgctcg gcttgctcat cctcgccctg caaacgctcg taggtggcca ggtgcagctg
    17761 aatatgcagc ggcaattgcg gatggaattc gggggccgca gcggcctgct cgccgccata
    17821 ggcatccagc aaacggctca gccagacgcc cgccagttca gtctgtccct gacgcagcca
    17881 cagctcacat ttggtcaggg tgatcatagc caggtagtag atcggcggaa tatcccagat
    17941 atgcatcagc cgttcggcct cggcgagctg ggcaaaggcc tcgggaaggt tgccgctgcg
    18001 cccttccata ctggccagca cgcaataacc gatcaacaca ctgatatcgc ggcaactgcg
    18061 cgcttcggca atcccggcca acaatcgctg acgcgcctgc tgtggttgca aacgcaaact
    18121 cagcagatag ccttcataca gagtcagccg cccgcgcgcg gcgtactgcc gttgcgccgg
    18181 cagactggcc agacgctgct gaccctgacg cacttcatcc agcgcgcgca gcacttcacc
    18241 acgggcctgc aatacccgcg cgcggtcgta atgcgctaga gcctcgaaca gcggattacc
    18301 gacacgctgc gccaactcca gagcctcgcg attcagcccc ctcgcgcgcc atagatcacc
    18361 gtgcacaatc gccaggttgg ccaaactgga gagacacatc aaacgctgcc cgtaacggtc
    18421 cagcggcaaa cagcttagtg cctcgctgca ataggcctgg gccttggcac tgtcaccgcg
    18481 cccccgtgca attacgccgc tcagggccag ccactgggcg agcaagcctt cctgctcgca
    18541 gcgctgacgc gccggcaaaa agcgcccgag ctgggcgaga agctcttcgg cggcatccag
    18601 ctgacaggct aaggccagtg cccagccata caacagaatc aggcgaggcg tactggtcag
    18661 cagactgtcc ggcaagtcca tcttccaacg cagcaaggtg gccacgttct gctcggccaa
    18721 caattgttct tcggagaggt tctgcaccag attggccgcc acatcaggct gaccggcacg
    18781 taacgcctgc tcgatggcct catccagcag tccttcagcg ctaaaccagc ggcatgcgcg
    18841 caaatgcagg cctggggttg gctgcggacg actgcgcagc aagtcggaaa acagatggtg
    18901 ataacgaaac catttgccct gttcgtccag cggcaccaga aacacctgat gcgcctgcag
    18961 gtgacggaga atcgcggcgc tgtcatgggc atcgcgcagg gcatcgcaca gctcggcgca
    19021 gaagcgctcc tgaccggctg tatcgtagag aaaggcctgt acctcggagg gctgtcgctc
    19081 gatcacttct tccagcaagt aatcatgaat cagcccatca gcaccttgcg ccagcgtcgg
    19141 tagagccgaa gactcctgca cctcactggc ggcaagcgac cacagccgca gccccgccac
    19201 ccaaccctcg ctgcgctgca atagaacagc cagcgcatcg tccgacagca cagcgccttg
    19261 tagcgcaagc aactcagtgc cttccgtatc ggtcaggcgc aaatcttgct catgcagctc
    19321 cagcaactgg cgcgacagac gcagacgcgc tagatgccag tccgggcgct ggcgactggt
    19381 caccagcatc actaaaccgg gcggcaagtg gcttaaaaag aactgcaggc agcggtcgag
    19441 caccgcgccc tgggccaggt ggtaatcatc cagcaccagc aacagtggct gctgaggatc
    19501 gaggcgcagc gccaactcat cgagcaggcc gtcgagccac tcttcaaagg caaacggctg
    19561 atgccgctga cgcagacgca gcaaccccag cgcctcctgc ccaacgcctg ggaaaaactg
    19621 ctgcaaaccg caaagcaagc gctcaagaaa gcgccccgga tcactgtcgc gcgcactcaa
    19681 gcccagccac aggctttgcc actgttcggg cagatgctcg cagaactcga tggccaatga
    19741 gcttttacca aagcccgctg gtgcactgac cagcagcagg cgccccgcta agccacccag
    19801 caaccgctca cacaacccag gtcgcgctac gtaactggcg ggtaacggtg gtcgatagaa
    19861 atgcccgccc agcacattgg gcgcggcaac caagccagga gttcgggaca aatcagtcat
    19921 caaccagttt cttgtagtag tcaggcagag gcactaccaa tgccccgcag actagcctct
    19981 tgatgtgcgc atttgaagcc cttgtggcca tcaggtagca aacggtaatg attgttcatc
    20041 catcggccac atactccctg gctttacgca agtataaaaa atggccgcga gccattttta
    20101 acgtcgcgca acgacagccc gaggggcggc cgccatggat ggacgaccat aaaaaaccgg
    20161 cccttgcggg ccggttcttt atgcagcatt actcaggttt agcgtacgcc agcctggcgc
    20221 agggccgctg gggtgtaatc gctttccttc gcagggtagt cgaactcgta agcacttttc
    20281 tcttcgttct tcatacccag agccaggtaa cgaccggaca gcaggtcata cagagtctct
    20341 acgctgtacc acggcacttg cttgtcgtag tagtactgag catgggcttc agccccacgc
    20401 cacagagtac cgcgaccgtc gtagtggtcg atggcagcag cttgccaggt atcttcgtcg
    20461 aggtagaagt cacgcttggc atagatatga cgctcgcccg atttcagggt tgcagtcaca
    20521 ttccatacgc ggtgcagctc gtaacgggtc aggtcctgat tgatatggcc agccttgatg
    20581 atctggtcat acttcagctt cggcgagtcc agacggtagc tgttgtaagg aatgtagatt
    20641 tccttcttac cgttgagctt ccactcgtaa cggtcaggtg caccgttgta catgtcgaag
    20701 ttgtcggaag tacgcagacc gtcagcagcg gtacctggac cgtcatagga cacctgcggt
    20761 gcacgacgca cacgacgctg accggcgttg tacagccacg ccagacgcgg ctctttcacc
    20821 tggttgaggg tttcgtgtac cagcagcacg ttaccggcca gacgcgacgg agcagttacc
    20881 cgttgtttga agtagaacag cacgttgctt ggcttgctgg tatcggcatc cttcagcgca
    20941 ccacggaagg tgaactcatc ctggaagtac accaggctgt aggagccgtt aggctgcgga
    21001 gtagcctggg ttaccagacg cttcacgctg ccaccacggt agcgggtgat gtggttccag
    21061 atggcttcca gaccgtcctt gggaatcggg aaggggttag cctgctcgaa gttctccaga
    21121 ccgttaccgc cctcgaccat cttggtgttg actgcgttgt tcttcgcagc cgccaggata
    21181 aagtcaggca tggtagcagt acgatgggtc gcgaacaccg gcattttgta gctttccgga
    21241 taacgcttga acatcgccag ctggccaggg gtcagcttgt ctttgtactg atcgacgttt
    21301 tgcgcagtga tggtgaacag cggctgctca ctcgggaacg ggtccgagag gaagcccgcg
    21361 ccatcggcag taccagcact agctggcaga ccgccagtcc aggctgggat cgaaccatcc
    21421 gcattaccag ccatttccgc accgactggc gtcagcgtag tacccagttt ggcagcttca
    21481 tccggcgata ccgccgccat cacaccgctg gccagcagcg agagtgccag ggcgccggtt
    21541 tgcagcagac tttttgttgt tttcatagtc atagtcgtcc tcaaaatctc gtccgcttag
    21601 aagttcacgc caaagctgag cgcaacgaag tcgcggtcaa ttgaagtgtt gtatttgcca
    21661 ccgaagaagt cggtgtaggc caggctggcg gtgtaggtgt tctgatactc agcatccagg
    21721 cccaggctaa tagccttgtt gccttcacca aacaaaccgt taggaccata gccatcaacg
    21781 tcatgcgacc aagaaacgct tggtttcagg ttcacaccgg caaatacatc cggataatcc
    21841 cagatggcgc gagcgcgata gccccaggaa gtgctggtaa caaaaccgtc gtcttcgcag
    21901 tacttagatt ggttctcgcg actagctgta ccaagtgtgc tgccattgag cgcgttacac
    21961 gtattgataa aagtagctgg gccaacctga gtatccggca atgggcctgg gccatagatc
    22021 ggatcgcgac cgtaacgagt gtcactagag ctctctaagc cccccacatg aacaataccg
    22081 acctcgccga ccaaggtaag acggctagca cccattacct gatcaaagaa gtgggtaaaa
    22141 gtggtctgaa actgagtaat ctccttacgc ttgtagccgt tcacttcctg gccaggttga
    22201 ccatcaatca gcgaagcgtt accaaacaag ccccccagtg ggttcacacc agcaaacagg
    22261 atatcagtgg tgttaagcag aactggcgca ttcggacggt agctaagctc accagcccaa
    22321 gaagtaccgg taggtaatgt tgtcgagaag ctcagaccat agagctgtat atcttcagga
    22381 tactcgagga aataattcga gtttcctgca acaaccagcg gcgcagcggc acctaaaaga
    22441 ccagcaacag cataaacatc agcagcagga ccagcagcgc taaacgctgg cgtacggcta
    22501 tgatagttca tgaaataagc accgaactca gtatctagct caggtgaaaa ataacggaaa
    22561 gccgtaccaa actgcccgct atcacgagca tcacgatcgc cagagcgagc aaccaatacg
    22621 ccttcgttgt tctgatcaat cactaagtct ggtcgaccaa ataagccgac aagagcagca
    22681 tttgtacctg cgcctgcagc tggattgttg ctcagaaccc gcaaattatc ggtacagcca
    22741 tcagcaatga tgtctggctg agcaaaaaat gtaccgcagt tgtcgactac agtctggtcc
    22801 cactcgagct gataaaaagc ctccatggag agattttcgg tcaggctctg ggagacataa
    22861 aacatattga ccgggatcag gccttctttg atctctgcgc ccggacgacg gaatgcagaa
    22921 gcatccacgg ggttgattga gttaatactg ttctggataa aagtactttc accccaacta
    22981 actacttgct tgcccaaacg gaccgaaccg ggctgatccg ctattgcata gttgtgataa
    23041 acaaaagcat cgagaatttg tgcacccgat gcctgagcgc cttctttacg gttactgtcg
    23101 ctaatatctt tgaacagacg gctttcgtct ttcagttcaa agtcatacca gtatttacca
    23161 cgaacaaata cgccggtatc accatatttc aactcaagat catgaatacc cttgaagatt
    23221 tttgaaaagg tttcgccgcg tttgaaattc tgacgcgaat cgtcagtggt ctgagataaa
    23281 ccgtcaccac cgttgttgga accgattaga tctggatccg caccacgtac cgaccagctc
    23341 gcgccaaccg acaaggacga atcgaactga ccttcgattt caccgatatt gaaagtaacg
    23401 ccgaatgcag gtgcggcgag ggtggatgcg aggctgacgg ccagtggcaa ttttgccagg
    23461 cgccaagttt gttttgtttt tgtcatcgac gctactccat gtgttttttg ttatggatgg
    23521 tgcggactat agccagcggg tacttctgct tgatccctct aaagtgtgat ttgcagcccc
    23581 caggcactct ggctcgcagg tttcggcgtg tttgacacga ccggcagagc caagaatggc
    23641 ttggaatgag caattagcaa gccaaacgct tgtttgactg accagtcacc aaaagcgacc
    23701 tggcgtgacg cgtattgaac agcccacaac gttacgcaaa ccgctcttgg aaaattctgc
    23761 aaaaccgcct gaaagccttt attcagctgg cgttggcaca actgcacggc aagcccacat
    23821 acgggcgaga gctgcgtctc gcccgtggta caaagtctta gacggttgag agaaacgggc
    23881 tacctgcagc ctgccactgg acaatatcca ggcgaatgcg tttcttatcc agcttgccga
    23941 cgctggtctt gggaacttcg gtaacaaggg caatttgtga cggtatcgcc cacttgttga
    24001 tgctgccctg atcgacaaaa ggtttgaggt gctccttcag ccctttggca tccagactct
    24061 ggccttcacg cagtaccagc agagcaaacg gccgctcgcc ccactgcgga tcgaccacac
    24121 cgaccactgc cacttcacgt accgcagggt gacgactgat caggtcttcc agctccaggg
    24181 aggagatcca ctcgccaccg gtcttgatca catccttgat acggtcgcgg atatcgataa
    24241 agccaaagtc gtcgatggtc gccacatcac cggtgtgcag ccagccgtgc tcccacagct
    24301 cagcgccctt ctccggctcg cggaagtagc cctgagtcag ccacggcgca cgcagcacca
    24361 gctcaccctg gctttcacca tcggccggta acagggtgcc atcggcggac atgattgccg
    24421 cttccaccag cggtaccggc acgccagcct tgatccggta agtggtgcgt tcatcttcgc
    24481 tgccggccat caattcatca ttgatatgcg cgcaggagat cagcgggcag gtttccgaca
    24541 tgccataggc agcggtcagc tggatgccgc gggacttggc ggtctcatac agcgagcggt
    24601 tgagcgcgct gccgccgatg atcatcttca ggccgccaaa gtcgtggccc tgagcccctg
    24661 gggcggccag aaccatctgc aaaatggtcg gcacgcagtg ggagaaggtg accttttcct
    24721 ctttgatcag gcgacagagc atgtccggtt catagcggcc agggtagacc tgtttgatgc
    24781 ccagcatagt ggccacatat gggatgcccc aggcatgcac gtggaacatc ggggtgatcg
    24841 gcatatacac gtcgttggtg cccagcaggc ggatgctgtc caagctgccc agcacgctgg
    24901 cttcggccat ggtgtgcagc accaactggc ggtggctgaa gtacacgcct ttggggttgc
    24961 cggtggtgcc ggtggtgtag aaggtggtgg cgaccgagtt ctcgtcgaaa tccgggaagt
    25021 cgtagcgcgg gctggcggcg gccagcaggt tttcgtattc accgaccaag ccgcccaggt
    25081 cggcggtttt ctcggctgca tcggtgagca gaatggtctt ctcaaccgtg gtcaactggc
    25141 tttcgatgcc tttgtacagc ggtacgaact cgctgttgac cagcacaaac ttatcgtcgg
    25201 catggttcat ggtgtaaaga atctgatcag ccgataggcg gatattgatg gtatgcagca
    25261 ccgcgccaat cataggaatg gcgaacatgc attccagata gcggtggctg tcccagtcca
    25321 tcacggccac agtgtcaccg gccttgaccc cggcttcgct gagcacattg gccaagcgcg
    25381 ccacgcgctc gttgagggtg gcgtagctgt aacgcagctg gtcgcggtag acgatttcgc
    25441 gggttttctc gtagcggata ccggacagca gcaggcgctt gatcaacagc ggcgcttggt
    25501 gtgcattgtc ggcgggggca atcaggcggg tctgcagcat ggcgttacct attgttggct
    25561 ttttcagtgt ggccaatcta gagcccccgc cgccggctca aatcagccca aagaatgatt
    25621 ttctgtgtga ctctttgggc atttttagtc acgcagtaga atcgacgcct cgcattcgat
    25681 gtgtttgacc tgcgatctac tacaaaaacg aggagtttcc tgatgtccga tatcgtcatc
    25741 gtcagcggcg cacgtacgcc gatgggtggt tttcaaggca gcctgtcgag cattgctgcg
    25801 gttgacctgg gtgcagccgc tatccgtgcc gcggttgaac gcgcaggtgt tgcgcctgcc
    25861 gacgtgcagg aagtaatcat gggctgcgtg ctgccggccg gcctaaagca gggcccggcg
    25921 cgccaggcgt cgctgaacgc cggcctgcca gctgccaccg gttgcaccac gatcaacaag
    25981 ctgtgcggtt cgggcatgaa agcggtgatg atggcgtttg attcgctcaa ggccggtagc
    26041 aataacgtga tgatcgccgg cggcatggaa agcatgtcca acgcgcccta catcatgagc
    26101 aaagcccgcg cgggcctgcg catgggccac ggcgagatca aggaccacat gttcctcgac
    26161 ggcctggaag atgcgcgcac cggccgcctg atgggctcct tcgcccagga aaccgccgac
    26221 cagtacggca tcacccgtga gcagatggac gcctacgcca tcgagtcgct gaaacgcgcc
    26281 caggctgcca ttgccagcgg tgcgctggat gcggaaatcg tcccgatcac cgtcagcggt
    26341 cgcaagggcg atgtcgtggt caaggatgac gagcagccgc tgaacgctaa cctggagaaa
    26401 attcccggcc tgaagccggc cttccgcaag gacggcagca tcaccgcagc caacgccagc
    26461 tcgatctccg acggcgcctc ggcgctgctg ctgatgactg ccgaagaagc cgccaagcgt
    26521 ggcctcaagc ctctggcgaa gatcgttgcg cacgcgaccc aaagccaaga cccaagcgag
    26581 ttcaccatcg cgccgattgg ctcgatcagc aacctgctga agaaaaccgg ttgggataag
    26641 gccgaggtcg acctgtacga gatcaacgaa gccttcgcca tggtcaccat gctggccatc
    26701 cgcgaacacg gcctggatca tgccaaggtc aacgtctacg gcggcgcctg tgcccagggt
    26761 cacccggtcg gctctaccgg ttcgcggatt atcctgacct tgatcaatgc gctgaaaaac
    26821 accggcggta aacgcggcat cgcttcgctg tgcattggtg gtggtgaagc gacggctgtg
    26881 gcagtcgaat tgatctaagc caaatggtgg aaaaacgctg cgcggttttc cacactacag
    26941 atgccaacgg gccagccttg cgctggcccg ttacgtttta gataccaatc ttgtccagcg
    27001 atttgccaac cttgcgccag aagctgcgct gcatgcgctc ggtttctcca ctggcggcaa
    27061 tctgctcgaa gggttgtatt tctcgggtgg tggcgatttc cgttgcggcc acttcttggg
    27121 aaaccagttg cagcgggcca tttccctgac tgggcgcctg ctcattgaca gccggtgggt
    27181 tgagctcgtc ataacgcagg tagtagatgc cgccagcttc ggcatccagg gtgaagctac
    27241 tgatcagagt gaagtccagc gggccatcgg cgaagaaagt ccagtgcgtg ccgaacagcg
    27301 gccggcgcat ctccagctgg taactggcga catcgaactc caacaccata taggcattgc
    27361 tcggcaggct gccgatgcgc gcgtgattga gaaacagcgc tggcgcttcc agctcctggt
    27421 cagcccagtc gctttgcggg cggtagaggt aaaccagggc atggttgtca tcgctgagcg
    27481 catgctcgcg gaacgcttca ccctcggttg caccaaaaaa cgcccctggg gtcgagcaac
    27541 cactcaaggt gaccaacaac agcactgtaa accagagcag accacgcatc gatagcatcc
    27601 ttattcgagt tataggccga gcatagcggt gccctgcccg gccgacacca aacaaaagtg
    27661 cgggcgaaaa agtgcggatc agcggcggcg gtcaagcaga ttgaccagca attgatccag
    27721 cgccccccaa atggactgct gcaaccgtcg ccaccagggt cgcgcacgcc actcggccag
    27781 agtaatttcc tggctatcgg caaagtccgc ctgaaagctg gccgccacgc tggcggtaaa
    27841 cgtgggatca agcgcctcca ggttggcatc cagattgaag cgcaggttcc agtggtcgaa
    27901 attgcacgag ccaacactga cccaatcgtc caccagcacc atcttcaggt gcagaaaacg
    27961 cggctgatac tcgaagatgc gtaccccggc cttgagcaac gtggagtagt aacgctgacc
    28021 ggcaaaacgc accggcgggt tatcggtgtg gtgcccggcc aataacagac gcacatccat
    28081 gccgcgcttg gcagcctggc gcagagtgcg acgcacgcgc caagtcggca gaaagtatgg
    28141 cgttgccagc cagatacgct gcttggagcc acgcaacgca cgcaccagcg agagcaagat
    28201 gtcgcgatac tgcgaggcat cggcataggc tacacgcccc aaaccttgcc ccaacgcagg
    28261 caatgccggc aggttcttca agcgcaacgg catacgcggc cgccagacga tccagtcccg
    28321 actcgcctgc cactggcgct caaacaactg ttgccagtcg gccaccagcg gcccgctgat
    28381 ttccaccatg acctcatgcc aagggctgct ggccttctcc ggctgccaga actcatcggt
    28441 cgcacccgtg ccgccgacaa aggcaaattg cccatcgacc agcagcagct tgcggtgatc
    28501 acggtgaaag ttgcgcaggc catgcttcca gcgcagcggg ttgtaccact gcacctgcac
    28561 gccggcagcc tgcaggcact gccgatccgc gctgtacaaa ccttgcgcgc caaaggcatc
    28621 cagcagacag cgcacggcaa cgccacgctg ggcagccgca cacagcgact ccagcaaggc
    28681 actgccgcag gcaccgcttt ctactagata aagttccagc tccacctgcc gctgcgcggc
    28741 gtcgatacgc gccagcatct gcacaaagaa ctgcggcccg tcgatcagca gcgtcagcgt
    28801 gttgccgcac cgccagggaa agacgttatg cggcatgaca gctcacgttg cagttgattc
    28861 tgcgcattgc gtctcacgca taaaaaatgg agacgccacc atagccggct ggcaaggcct
    28921 tgcaaaccct accagccgtc tctcgctatc aggactcggg cagcgcagtc gcttttggca
    28981 atagctggca accctgacgt aggcccaacc attgcgcact ctgggtgctg cccaggccac
    29041 gcgccgtcac gcgcttgttg gcttgatcat cgaagaagcc agagagcgaa acgtactgcg
    29101 ccacatacgc ttcgcagtaa tcggccggat tgttcgccac atagcggcag gagcagtatt
    29161 ccttggccga gtaggcaccg atgatccccg gaaaagcctg caagtgcgcg cggttctgcc
    29221 acgccagggc aacgagtagc aacacaatca gcaacagcag actgctgaac ggacgacgac
    29281 gaatcatggc tgcacctcat cggccaacgc ggcgagaacc agtttgagga aatggttgcg
    29341 atcaaagctg ccatcgcggt catcggcgta gcgcacgatc agcaggttct cgctggcgat
    29401 cacgtacagc ccctgccccc aatgcccgga cgccaccaga gtgtcctcgg gcgcatccgg
    29461 ccagggcctg ctcgtcccag ccaccgctgc gttgagccac cagtgcccgc cgggcaccgc
    29521 ctcaccaggc tgttcggcct gtggctgata gttggcaaac ggcgtgcgat tgaactcgac
    29581 ccaggcttta ggcagcaact gctgctcacc ccagcgacca tcgcgctgca tcagcaggcc
    29641 gacacgggcc aggtcacggg cgctcatata gacgtaggag gagccaacaa aagtgcccgc
    29701 tgcatcacgt tcccagacgg cagtcttgat ccccaacgga tcgaacagcg cggtccaggg
    29761 gtaatcggtg taggcctcgg cgcccaccat gttcttcaac gcggcggaca gcacattgct
    29821 gtcaccactg gagtaacgga agcgctcgcc cggttctgca tcggcgctgt gcgcggcagt
    29881 gaacgcggcc atatcactgc gaccacgggt gtagagcatc gccaccaccg aggatttcag
    29941 cggcgcgtat tcatagtcct cctgccagtc cagtcccgag gcccagttga gcagatgacc
    30001 gaccttgacc tgcgggtgtg cggcaaacgg cgggtagtaa tcggccaccg gcgcatctag
    30061 acggaagcgc ccctggccgt aggccacgcc cattgtggtg gcgagcagac tcttggccat
    30121 cgaccaggtc agatgcgggg tcgcggcggt ggtaggcgca gcgtagcgct cgtaaaccac
    30181 ctggccatcg cggatcacca gcagggcgtc ggtgcgcaca cctgtgcggc tggcgtcatc
    30241 acgaaccgga aaggcgtagc tttccagttc gctaagcgcg ctgctaggcg cggccaactg
    30301 gctgtcccag tcttcacctg gccagttctc agcgtgcgcc gtcgcgcagc tcaggccgaa
    30361 ggcacacgcc aggatcaacc gccgtgcggg aaagagagtg aaggacattg cgctgcctct
    30421 gcggggatcg aaggcggcac cctagcatgg cgctgatgac gacaaaaagc gccgaacacg
    30481 cctgtttacc cggtgattcg gcgcaaaaag tcactgcacg gaaatccgct acttgggcag
    30541 gcgatagtcc tccggcccca tcagatccat gccgcactcc aggctctcga tccagtagag
    30601 gaaggcattg atcatttccg cgccgacaaa aggccgcccg tgttgtggca cgatcatcgc
    30661 cacgtccatg tcacggacca ttgccgccca cagccggcag atcttgttac tggccatata
    30721 gcggcggtga aagccgagca tattcggcac atggttaacg aagtcgctca ccgggtatgc
    30781 gtcgtcgacc atcgaggcgc ccatatcgcc ggaaaacagg atcttgctca ccgggtcata
    30841 cagctggaag ttgcccaccg agtggagaaa atgcgccggt actgccttga gcgtgcattt
    30901 gcctagggca aacgactggc cgcgatccgg caacgcgatg atccggtcga aggtattgat
    30961 gccgcggctg agggccaggt agttggccgt caggtgcggc agaaaacgtg cccagagttt
    31021 ggagcagatc acccgcgctc gagtatgcag cagccactta tccagcgaag cgatgatgtc
    31081 cgggtcctgg tgcgaggcga agatataggt caggtcctgc accgggatgt gcttggacag
    31141 ttccagcgac aacggtgtat aggtcagatc gccacccgga tcgagaagca ggtattgctc
    31201 gttgtcggtg atcagaaact ggttggactg cacgccctcc ccgctgacca ggtcatcgaa
    31261 catcatgcat tgatgctcgc cgttatcgaa caacactatg ggctcgcgtc gcatggcagg
    31321 ctctctcccg aaagcccgcg agcttactgg cgcagcggca cggccttact gatctggatc
    31381 aagccggcac gccatgccga gttaaaggtt gataccgagc acagcctgcg ccttctgcag
    31441 acgtttgctg cgcgcgctgc cggcaatcgc cagcaggcct ttgtcgcgct gccaaagcgg
    31501 cgcccagtgc gcatcaccgg cggtaaaggc gttgtccaaa tcactgcgca ggctatcgaa
    31561 ctgagcaaac agcccggcca gcatcaggtg gctatccgct gcacttgggc attgccgcgc
    31621 cacttccgcg cagccggcca gcagaccaag caggcgcagc tgcagggcct gcggccaggc
    31681 cgattcctgg cccacgctaa acagccaagc ggcgagcgcc gcaagtacgt gggtgtcttc
    31741 cagagtacgg aagggtttta cgtagtcgtc ccagccatcg ccggccagcc gttcgcactg
    31801 cgcgccctgc agatgtaggc ggccatggct gacatccggc atcagcggca atgcgggtaa
    31861 ggtctcgatc tgcacccccg gcgcaccatt gcgcaccacg ccaagagcca gctgtaccgg
    31921 cgcgccgccg gtttcctcac gggccgcaac tagcagccag tcagcggctt cggcagcggt
    31981 gacgaaatcc ttgcggccat ccagggtcag cgcgttgatc cgcgtgctca tatcggccgg
    32041 acgggtgctg cggttctcgg ttacgcacag cgcgcccagg gtccagggtg cggccggcca
    32101 gagtgcgcgc aacgcgcctt gatagccggc gagaaacgcc agccccggcg tcgctgccag
    32161 ccggccgccg agcaaggcca gctgcaacgg gggcgggtta ccgatgcgtt cgagcaggct
    32221 ggcgtaccac tcctccagcc ccgcgaccgg cagacgcgct tgcgggctca acaactgttg
    32281 ccagggcatg cattgctcct tgttggggtt attgaagtca ctgtcacaca agcatcacca
    32341 aagattcacg gaggtgacac cgccgctacc tagcctgagc ttgcccaaca agatcgaccg
    32401 gctgcaaggc aaaaagccgg ctaacggaga tgacggcatg acccctatgg cacttaaagc
    32461 tactcccgca cgtcgcctgc aggccgagcg cttacacggc gctgccgccc tgcgcgaagc
    32521 tcaggcgctg cgctttcgcg tattcagcgc cgaattcgat gccaagctca agggtgccga
    32581 actgggcctg gacatggatg actatgacat tcactgccag cacatcggcg tgcgtgacct
    32641 caacagcggt gaactggtgg ccaccacgcg cctgctcgac caccgcgcag ccgccagcct
    32701 ggggcgcttc tacagcgaag aagaattcag cctgcacggc ctgcctcacc tcgaagggcc
    32761 ggtgctggaa atcggccgca cctgcgtcga cgccgcctac cgcaatggcg caactatcgc
    32821 cgtgctctgg ggcgagctgg ccgaagtgct caacgaaggt ggttaccgct acctaatggg
    32881 ctgcgccagc atctccatgc aggacggtgg catccaggcc caggcgatca tgcagcgcct
    32941 gcgcgaacgc tacctgtgca ccgaacacct gcgcgccgaa ccgaaaaccc cgctgccagc
    33001 gctggatgtg ccgagcaacg tgatcgccga aatgccaccg ctgctcaaag cctatatgcg
    33061 cctgggtgcg aagatctgcg gcgagccgtg ctgggaccaa gatttccagg tcgccgacgt
    33121 gtttatcctg ctcaagcgcg acgagctctg cccgcgctat gcccggcact tcaaggcggc
    33181 cgtttgaggg caggtcaacg gctgctgcgc gtcggccctg cggcgttaaa aacaggctcg
    33241 gaatgctcat gtacaaaagt acactccgct tcctcgcctg tttttgcctt gcaggactct
    33301 agctcgcgag cctttaaaca agttatgagt ggagttgcac aactcgccgg cactctgtag
    33361 acagtgccgg cgtttgtgtt tttgccgcag gagagcttga tgaagaaatt gcgtttgtac
    33421 ctgcgcctgg cgcgcctgct ggcagtgatc agcctcggca ccctgctggc ggcgggcatc
    33481 accctgctcg aacgcctggt ccgacatgac ctgatgccga cccgccagcg cctgacccgc
    33541 tggtttctcg cgcgcctggc tgcggctttg ccgttccgct tgcaggtgca gggcgaactg
    33601 ccgcgtcagc cgatgctgtg ggtcagcaat catgtgtcct ggaccgatat tccgctgctc
    33661 ggcatgctca ccccgctgtc ctttctgtcc aaggccgagg tacgcacctg gccggtggcc
    33721 ggctggttgg cgcacaaggc cggcaccctg tttatccgcc gcggttcggg cgatagcaac
    33781 ctgcttaatc agcagttggg caaccacttg caacgcgggc gtaacctgct gatcttcccg
    33841 gaaggcacca ccaccgacgg cctcggcctg cgcacctttc atggccgttt gctcagcagc
    33901 gccatcgaca gcggcgtgtg tgtgcaaccg gtggccatcc gttacctgcg cggcggcaag
    33961 ccctgcagcg tggccccttt tatcggcgat gacgacatgc tctcgcacct gctgcgcctg
    34021 cttgacagcg agctgagtga agtgcaaatc cagctgctga cgccgattgc cagcagcgat
    34081 ctgaatcgca atgccctggc ccgcgcagcc catcaggcga tcagtcacgc gctgtttggt
    34141 gcagcagccg cacctgcaac agcaaggacg gaaacagaaa gccaggcggc ctaaccagca
    34201 agcccacgct cgcgggcgac ataagcctgc aactcaggat agaactggcg aaaatcctcc
    34261 aacagcggct gatacagccg ctccagctcc cccaggccgc ctatcagcaa gccgggccgc
    34321 gacagccgtc ggtcaatccc cgccaccacc tgttccagca cggcgaactc gcggtaactg
    34381 cccagccaat cctgtgcggc catgcgcggt gcgatcagcg ccagacgctc gggcaagcgc
    34441 ggctcggcgg ccaacacctg ataaacccgt ccggtaaagc gcggcagcgg ctcggcggca
    34501 tagtcggccc agtgcagcgc caggcagtga tcgaaaaaca catccagcaa gatcccgcta
    34561 tagcgccgcc gctcgacggc aaagcgcgcc cgcgcctgca attgcagcgg gtggctgtcg
    34621 gtgaatacat cgatacgtcg gtgcaggcgg atggccgcct cgatatccgc cggccactgc
    34681 ccggccagcg gccctttgac gaaatcacca tacaggctgc ccagcagttg cgccggcgcg
    34741 tcgccgccca ggtgtagatg cgcgaggtag ttcatggcca cagcttacac atcagcacgc
    34801 gtgatgaaga ctatcgccac gaacgatctg tatatcgcca acaaccgata taaggttcgc
    34861 cccatcgcga tatgacgtta cagcacacag agaacgcccc tatgcagctc gacattgacg
    34921 aggtcatcaa ggccctggcc cacccggtgc ggcgcgacat cctgcactgg ctaaaggacc
    34981 cgcaggcgta tttcgccgac caggaccatc cgctggaaat cggcgtatgc gccggcaaga
    35041 ttgaccagcg caccggcctg tcgcaatcca cggtgtcagc gcatctggcg accttgcaac
    35101 gcgctggcct agtaagcagt aagaaggtcg gccagtggaa cttcttcaaa cgcaatgaag
    35161 agaccatcca ggcgttcctc aaccacatca gccaagcgct ctaaaccaac ttttacattc
    35221 acttttttca ggagctgacg gcatgcctgc agcgcttctc gtcctcgctt tgtccgcgtt
    35281 tgccatcggc accacggaat tcgtcatcat gggcctgctg ccagaagtgg ctagcgacct
    35341 cggcgtatcg attcccggcg ccggctggtt ggtcaccggt tacgccctgg gcgtggccat
    35401 cggtgcgccc tttatggccc tggccaccgc cagctggccg cgcaaagcct cactgctgac
    35461 gctgatgagc atctttatcg ccggcaacct gttctgcgcc ctggccagcg attaccagat
    35521 gctgatgctc gcccgggtaa tcaccgccct ttgccatggc gcgttcttcg gcatcagctc
    35581 ggtggttgcc gccagcctgg tgccagccaa tcgccgcgcc tcggcagtgg ctttgatgtt
    35641 caccggcctg accctggcca acgtgcttgg cgtaccgctg ggcaccgcgc tcggtcagta
    35701 cgctggctgg cgctcgacct tctgggccgt gaccctgatt ggcgtaattg ccctgatcgg
    35761 cctgtggcac gtgttgccca gcaagaccga tgaagccaag gccgacttcg ccagcgaagt
    35821 gcgcgccctg cgcggtgccg gcctgtggtt ggcgctgagc accacggtgc tgttctccgc
    35881 ggcgatgttt gccctgttca cctatatcgc cccgttgctc ggcgaggtta ccggcgtatc
    35941 gcccaacggc gtgacctgga ccctgctgct gatcggcctc ggcctgaccc tcggcaacgt
    36001 actgggcggg cgcctggcgg atcgcagcct gaaagccacc ttgatcggcg tattcgtcgc
    36061 catggcggtg atttccaccc tattcagctg gaccagccag gcactgatcc ccgccgaaat
    36121 caccctgttc ctctgggcca ccgccgcctt cgctgcagtg cctgccttgc aggtcaatgt
    36181 ggtggccttt ggcagcggtg caccgaacct ggtgtcgacc ctgaatattg gcgccttcaa
    36241 cctcggcaac gccctcggcg cctgggtcgg cggcctggtg attgccgaag gcttcggcct
    36301 gacccgcgtg cccctggcag ccgccgccct ggccgtactg gcgatgcttg ccaccctgct
    36361 gacttttagt acccgtagcc ctgaacccca acttgcactc aacgaataac ctgagaggac
    36421 cgtcccatga ctacgctttt cgacccgatc cagatcggcg acctgcaact caacaaccgc
    36481 atcatcatgg ctccgctgac ccgttgccgc gcagatgaag gccgtgtgcc caacgcactt
    36541 atggccgagt actacgtaca acgcgccagc gccgggctga tcatcagcga agccacctcc
    36601 gtgactccga tgggcgttgg ctatccggac actcccggca tctggtcgaa tgatcaggtg
    36661 cgcggctgga gcaatatcac caaggccgtg cacgctgctg gtggcaagat cgttctgcaa
    36721 ctctggcacg ttgggcggat ttccgacccg ctgtacctga acggtcaact gccggttgcg
    36781 ccaagcgcca tccagcccgc cgggcacgtc agcctggtgc ggccgataaa agacttcgtc
    36841 accccgcgcg ccctggaaac cgaggaagtc gctgacatcg tcgaggccta tcgcctgggc
    36901 gcggaaaacg ccaaagccgc aggctttgat ggcgtggaaa tccacggcgc caacggttac
    36961 ctgctcgatc agttcctgca gagcagcacc aaccagcgca ccgaccgcta cggcggcaac
    37021 ctggaaaacc gcgcgcgcct actgctggaa gtcactgatg cggcaatcga agtctggggc
    37081 gctggccgcg ttggcgtaca cctgtcgcca cgggccgacc tccacgacat gggcgacgcc
    37141 aaccacgccg agaccttcac ctacgtcgct cgcgaactgg gcaaacgcgg catcgccttt
    37201 atctgcgccc gtgagcagga agctgacgac agcctgtcgc caagcctgaa agaagccttc
    37261 ggcggcgtat ttatcgccaa cgagcgtttc accaaagacc aggccaatgc ctggctggct
    37321 gaaggcaagg ccgatgcggt ggccttcggc gtgccgttta tcgccaaccc ggacctgccc
    37381 gcgcgcctgg cgcaggatgc tgagctgaac gcgccgcacc cggaaacctt ctacagccaa
    37441 ggcgcggtcg gctacctcga ctacccaacg ctgtaaaaca gagatccggc ttttcagcca
    37501 cccaggacgc ccgatgctca gcatcgggct gctccccgta catacccact gagtgatcgg
    37561 ttcacgccgt cactcccagc cagcacccgc tgatttgcga gccaaatgcg tgcaagcccg
    37621 ccagaatttg caccgcagca gaccatcctg ttcactcacc acaacgtttt atcgctgaaa
    37681 gcggtaaata acgcatttaa ttgtgcagtt cgcacaaccc tccttgtccg tctcgcccag
    37741 ctttatccgc ccgcctgagt tggcacgcgg cctgctatca ccaggttctt cccccacctg
    37801 gagcgatgaa catgaaccaa gcccagcaag acagcgacta ccccctgagt gaagtgccca
    37861 gtgacgctcg taaagggctg ctgtcgacgt ccattctgtt gttcggcttc accttcttca
    37921 ccgccacgat gttcgccggc ggcaagatcg gcatggcctt cgacttcacg accctgctct
    37981 gggcagcggt aatcggtaat ttgctgctcg gtctgtacgc tgccgcgttg ggcctgatcg
    38041 cctgccgcag cggccttaac tcggtgctga tggggcgttt ctgctttggc gagatcggca
    38101 gcaagctgtc ggactttctc ctcggcttca cccagatcgg ctggtatgcc tggggcaccg
    38161 caacgattgc catcgtcctg gtacgcttac tcgatctacc cgaagggctg actatcccgc
    38221 tgatggtgct gtttggcttc ggcttttgcg tcaccgcttt tattggctat cgcggcctcg
    38281 acctgctgtc gcgcgtggcc gtacccgcca tgctgattct gctggtctgc tcgctgtgga
    38341 tcgccacccg cgatgcaggc ggctgggcag cactgctggc catcgaaccc aaggacgaca
    38401 tgaccctgag catggctatt accctggtct tcggcacctt cgtcagtggc gccacccagg
    38461 ccaccaactg gacccgcttc gccaagagcg gtcgcgtggc ggtgctttcc agcttgatcg
    38521 gtttctttat cggcaacggc ctgatgatca tcgccggcgc ttacggcgcc atcgtctacc
    38581 agcaaccgga cgtggttgaa gtattggtgc tgcaaggtct gtcgatggcc gccgtggtga
    38641 tgttgttcct caacctgtgg accacccagg acaacaccat ctacaacttc gccgccgccg
    38701 gctgcaacat gctgcgcagc aaacgccgca agaccgtgac gctggtaggc gccggcatcg
    38761 gcacactgct ggcaattgcc ggcatgtacg agatgctgat tccgttcctg attctgctcg
    38821 gctcgatcat tccgcccatt ggcggcgtaa tcatggccaa ctacttctac tgccataagc
    38881 aaaactaccc caagctggtc gatgcgcaac tgccggcctt taactgggcc gggcttggcg
    38941 cttacctgat tggtgcccta tgcgcttacc tgtcgccctg ggttgcacct atcgtcggca
    39001 ttctggtcgg tgcggctgcg catatcgtgc tgtttgaagt gcagcgggta ttcagcggtc
    39061 agctccaggc caaagccaac ccatgagcct acgtgtcgcg gacgtactcg cactgcccgg
    39121 cctgcaaagc ctgcaactac gggccggtgc agccgggttg gacaacccgg tgcgctggcc
    39181 ttacgtggcc gagaacgcag gcattgccga ttgggtgatg ggcggcgagc tgattttcgt
    39241 caccggcatc aaccacccgc gtgatgaagc caacctgctg caactggtac acgaaggcca
    39301 ccagcaactg gtggccggcc tggtcattct gaccggtgag gaatttatcc agagcattcc
    39361 cagctcggtg atcaacgagg cagaacgcct cggcattccg ctgctggagc agccttacca
    39421 gttgaaaatg gtcatcgtca cccaggccat cggtacggca ctggtcgagg agcaagtgct
    39481 cggccgctcg cgcctgcatg tgctggagca actgctggaa ggtgactatc gctcgctgga
    39541 tgtgctgctg cagcgcgccc ataacctgcg cctgccactc gcggtgccgc gccaggttgc
    39601 gctgttcaaa cttgaaggtg gtgagcgctt gtttaccgcc gagcagccgg aacaggccga
    39661 acgccagctg caagccaacc gccagcaact gcaacgccag ctggaacaga gcctggggac
    39721 gctgggcgat gctctggcac cgatctgcca aggtgaacac tggatcgccc tgctgccagc
    39781 ccacgacagc ccgcatgagc aacgtaaccg ccgcgccatg atgcgcctgc tcgaacagct
    39841 caatcagcaa ctggcgccgc aacgtctgtt tctcggactg agcgacgcta atcatcaccc
    39901 ggcacgctat gcccacggtt taagcgaagc gcgccaggcc ttggtcgcag cgcaatcgtt
    39961 cccggaacgg ctggggctgt gctgcttcaa cgaactgggg gtgatcgaat tgctcggtgc
    40021 gatccgtgac cgcaccctgc tcgaccgctt cgtcgacaac gtgctcggcg cattgatcgg
    40081 cgacgattca cgtcatgaag ccgtgctgat tccaaccctg gaagcctggt tccatgaaaa
    40141 cggtaatctg gccctggcgg cccaacgcct ggccgtacac cgcaacaccc tgggtcatcg
    40201 actacaacgt atcgaagccc tgagcggttg ttcgtttgac gatccgcatg accgtctgaa
    40261 catcgccgtg gcactgcaaa tctggcg
//
LOCUS       DASRUM010000003         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1507354, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000003 DASRUM010000000
VERSION     DASRUM010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507354"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..917)
                     /locus_tag="VFF22_00255"
     CDS             complement(159..917)
                     /locus_tag="VFF22_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009046352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HZX15287.1"
                     /translation="MIWDIGWSGALLLIIALAVASVRILREYERGVVFLLGRFWKVKG
                     PGLILIVPGIQQMVRVDLRMIVMDVPSQDVISRDNVSVKVNAVVYFRVVDPEKAIIQV
                     ENFIEAISQLAQTTLRSVLGKHELDEMLAERERLNIDIQQILDAQTDAWGAKVANVEI
                     KHVDIDESMVRAIARQAEAERERRAKVIHAEGELQASEKLLQAAQVLAQSPQAMQLRY
                     LQTLNAIAGDKSNTIVFPLPTEWIDSLGKFSRGG"
     gene            complement(920..2263)
                     /locus_tag="VFF22_00260"
     CDS             complement(920..2263)
                     /locus_tag="VFF22_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HZX15288.1"
                     /translation="MKRSVGYFILLALLVILPLAAFARTVVVAEIDGAISPASAAYFL
                     RAQDEAKRAQAELLVLRLNTPGGLDSSMREMIQGILASPVPVATYVAPSGARAASAGT
                     YLLYASHIAAMTPGTNLGAATPVAIGIGGDSGKPPPADSADGKERAPAASSMEKKAMQ
                     DAAAYLRSLAQLRGRNAEWAEKAVREAESLSADEALKLKVVDFVAADLSDLLRQADGR
                     SVKMAQGERRLELEGAAIVTIEQNWKERMLAAVADPNIALILMMLGIYGLLFEFYTPG
                     FGVAGVIGAISLLLALYALAMLPINATGALFLLLGIALMATEAFVPSFGAFGIGGIVA
                     FVAGALMLIDADIPGLEISLAFVLPLAVTSALILVGIGTFALRARHRPVVTGSEAMVG
                     GTAVALEDFEHEGWVQALGERWHARSETALTRGARARIVAVEGLTLVVQPEQKGE"
BASE COUNT          454 a          763 c          659 g          436 t
ORIGIN      
        1 gcccctacgc cacatcagtt tgaatcgcac ccatttcaac ggattatgcc gcttttgtag
       61 gtgcgaattt attcgcacct acaaaccact caactgagga atttatggaa aagatgggtg
      121 gccttcacag ccatccggcg cggagaccaa agcatgccct atcctccgcg gctgaatttg
      181 cccagtgaat cgatccattc tgtcggcaac ggaaacacga tggtgttgct cttgtcgccg
      241 gcaatggcgt taagcgtctg cagatagcgc agttgcatgg cctgcggcga ctgcgccagc
      301 acctgggcgg cctgcagcag tttttccgaa gcctgcaact cgccttcggc atgaatcacc
      361 ttggcgcgcc gctcgcgttc ggcttcggcc tggcgggcga tggcacgcac catggactcg
      421 tcgatatcca catgcttgat ttccacattg gccaccttgg caccccaggc gtcggtctgc
      481 gcatcgagaa tctgctggat gtcgatgttc aggcgctcgc gctcggccag catttcatcc
      541 aattcgtgct tgcccagcac cgaacgcaat gtggtctgcg ccagttgtga aatcgcctcg
      601 ataaagtttt ccacctgaat gatggctttc tccggatcga cgacgcggaa atacaccacc
      661 gcgttcacct ttaccgaaac gttgtcgcgc gaaatcacgt cctggctcgg cacgtccatc
      721 acgatcatgc gcaggtccac ccgcaccatc tgctggatgc ccggcactat gagaatgagg
      781 cccggtccct tcactttcca gaaacgcccg agcaggaaca ccacgccgcg ctcgtattcc
      841 cgcaggatgc gtacgcttgc taccgccagg gcaatgatga gcagcagcgc gccgctccag
      901 ccaatatccc agatcatgat tactctcctt tctgttcagg ttgcaccacc agggtaagcc
      961 cttccaccgc cacgattcgt gcccgtgcgc cgcgtgtcag ggcagtctcg ctgcgtgcat
     1021 gccagcgctc accgagagcc tgcacccagc cttcgtgctc gaaatcctca agtgctaccg
     1081 ccgtgccgcc gaccatcgcc tcgctgccgg tgacgaccgg tctgtgccgt gcgcgcaacg
     1141 cgaaagttcc gatgccaacc agtatcaggg cgctggtgac ggccagcggc aagacgaagg
     1201 cgagcgagat ttccaggccg ggaatgtcgg cgtcaatcag catcagcgcg ccggcgacga
     1261 aggcaacgat accgccgata ccgaaagcgc cgaagctggg cacgaaagct tccgtcgcca
     1321 tcaacgcgat gcccagcagc aggaacaagg cgccggtggc attgatcggc agcatggcaa
     1381 gcgcatacag tgccagcagc aggctgatgg cgccgatgac gccggccact ccaaagcccg
     1441 gcgtgtagaa ctcgaacagc agtccataga tacccaacat catcaggatc agcgcgatgt
     1501 tcgggtcggc caccgcagcc agcatgcgct ccttccagtt ttgctcgatg gtgacgatgg
     1561 cggcgccctc cagttccagc cggcgctcgc cctgcgccat cttgacgctg cgcccgtctg
     1621 cctgacgcag caggtcggac agatcggcag cgacgaagtc caccaccttc aatttcagcg
     1681 cctcatcggc tgacaggctc tcggcctcgc gcaccgcctt ctccgcccat tcggcgttac
     1741 gaccgcgcag ctgggcgagg ctgcgcaaat aggcggcggc atcctgcatg gcttttttct
     1801 ccatgctgct ggcagccggt gcgcgttctt ttccgtcggc agagtcggcc ggtggcggct
     1861 tgcccgaatc tccgccgatg ccgatagcca ccggcgtcgc ggcgcccaga ttggtgcccg
     1921 gcgtcatcgc ggcaatgtgg ctggcgtaaa gaaggtaggt gccggcgctg gcggcgcgcg
     1981 caccggatgg cgctacataa gtggccaccg gcacgggtga ggcgaggatg ccctgaatca
     2041 tctcgcgcat agatgagtcg agcccacccg gtgtattgag cctgagcacc agcaactccg
     2101 cctgcgcccg cttcgcctcg tcctgggcac gcagaaaata ggcggcactg gccgggctga
     2161 tggcaccatc tatttcggcc accacgactg tcctggcaaa ggcagcaagc ggcagaatca
     2221 ccagcaaggc cagcaagatg aaataaccca cggatctttt catgtatcgc tccgatgaac
     2281 gctacgtggc tcactgtaat ccgccaaccg gg
//
LOCUS       DASRUM010000004         7899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1507463, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000004 DASRUM010000000
VERSION     DASRUM010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507463"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="VFF22_00265"
     CDS             <1..1236
                     /locus_tag="VFF22_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZX15289.1"
                     /translation="RARSGYFVRELARHSLAEPETSPQLMQTTEVDVSELVFSVLASL
                     KDPGTVPFGSAFPSPQLFPLPRLARSMAHSLRDMQPQAVIADMTEGNPNLRRQIALRY
                     MVSGVMLPLEELVISSGAMEALNLCLQCVTQPGDLVAIEAPAFYACLQVLERLQLKAV
                     EIPVNPREGIDLDVLSERLAGLPIRACWFMSSLQNPLGASMSDDKKASLYQLLQRHQV
                     PLIEDDVYAELYFGSQPPKPVKSFDRDGLVMHCGSFSKCLAPGYRVGWVAGGRYAEQI
                     SRLKLMTTISPSVPAQAALADYLQHGGYDRHLRKLRHALEAQQASMLASAARHFPAST
                     KVTRPSGGYFLWFEFPEQVDALRLFQLALAQGISLAPGPIFSATRRFGNCARLNYGHP
                     WNAESEQAMATLGRILASF"
     gene            1581..3218
                     /locus_tag="VFF22_00270"
     CDS             1581..3218
                     /locus_tag="VFF22_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017906885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX15290.1"
                     /translation="MQSQLSIAQRLSLGFGLILSLMILITLIGVQRVGFIDETLTSVS
                     EGASLKQRYAINFRGSVHDRAIAIRDAVLVDNDAALNRQLAEIQRLKDFYQESAQAMS
                     TLLREQAATDTEQRLLSDIQTIERSTLTLTEQLIDLRRQGDTDAAKSLLLEQASPAYS
                     EWLKRINAFIEHEEQGVVQDISAVREAASGFRLLILLVTAAAVLLSIGVSLIIINKLK
                     STLGAEPHEVADVIQRLAKGELNQRIQTRYPDSVMGALQDMVKHLAETITQVRSAAGE
                     LTLASDQLLTTSDSNNQQIRLQSSEAEQMATAINQMAATVNEVANYAASAASATRNAD
                     HEVATGNQVVSATASAIQNLARTLESAAESVQQVSTDSESIEKIIEVITSIAEQTNLL
                     ALNAAIEAARAGEHGRGFAVVADEVRSLATRTQDSTREIRGMIGKLQEGAGKAAEVMR
                     NSRQLAQQTVEQTGQAETALNKIRSEVTAINEMNAQIASASEEQSAVAEEVNQNINRI
                     HSATLETSSGSEQVAASSRELATLANRLTDKVSFFKL"
     gene            3324..4385
                     /locus_tag="VFF22_00275"
     CDS             3324..4385
                     /locus_tag="VFF22_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZX15291.1"
                     /translation="MDSITQAVLGATIQGALLGRWQGRKALLYGAMLGTLPDLDVVID
                     YGDAVADMTYHRGFSHSLFVLTGLALLLTWLTRRFRHNPGYSAQRLFITLWLVLITHP
                     LLDSFTSYGTQLLWPLTPIPTAWSSIFIIDPLYTVPLCIAVALGLLYGLRDKAAKAPA
                     LALLVSSLYLGFTLAGKHMAEQRVEAELARQGIEAQQIFSTTTPFNSLLWRVIVLDGE
                     DYHEALVSWFDNTPPTLARIPRGTHLAAALVDSTAHARLAWFTNGVLRYDQLGEQLVV
                     TDIRLGMTGFHPFRFDFATLENGQWRVHDDIQRLPMDRGDLSRLQLLWARIWQPKVQV
                     PLLVWADELRKAPLASSTQ"
     gene            4461..6068
                     /locus_tag="VFF22_00280"
     CDS             4461..6068
                     /locus_tag="VFF22_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX15292.1"
                     /translation="MNIRQKMIACGAISVLAATLLGSIGFWGQTRLASALNENQLSVS
                     ALRNHLEGDMMHDALRADVLAAFYIDPTDSAAVTQVRDDLRDHSEWFTRTLNDNAKLP
                     LSADIRQAISQSQPALARYIQQAEGIVNLALTDPQAARQQMAAFDASFGDLEEKNEAL
                     SGLIEAHSAQTRSQGEAAVQQAAWLLIGGILVVCCLLCLLTTQLMKAVLHPLEKIIEV
                     ARNIAQGNLRSTISVDSNDEAGQLQQALADMQGNLRQMIDGIRQEGEQLQQTARNLNG
                     ASQSIVRSTTEESQSASSMAGAMEQMMQNIEQIAGHARNAQEISSHSEHLASNGGQVI
                     MGVVDGMSRIAEAVNESSATITALGESSEEIHSIIQVIKSIAEQTNLLALNAAIEAAR
                     AGEAGRGFAVVADEVRNLAARTAQSTQEITGMIERIRTSTEQAVNSMQTGVTRVNDGV
                     TLARQAGESISEIRGGAQRAAAVVEEISQTIDEQSRASSEVAQQVEHIAQMSQQNSRT
                     VNELADAAQSLDRVARSMQSAVLQFQT"
     gene            complement(6079..>7899)
                     /locus_tag="VFF22_00285"
     CDS             complement(6079..>7899)
                     /locus_tag="VFF22_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX15293.1"
                     /translation="GHASTSGLSAIDAQVLQARRAERDFALRQTSEAAAQVHEQLDRV
                     SSAVAQQLAAASTADQARLKSMQQAIDDYRRQFDSFVEQQNNARAARTQMREAAGEAR
                     DQFDVIELDMYDAVRELRLAGDKLRGSDPLTLAETASGLSKRMLDLRGHESQYIIDGS
                     AEALEEWTYISDDLQTVARSLMVWLDDSQKGAIETALKALTLYQQAFANYQQMREQSQ
                     QSAAAMVERAQAVLALVEEAKAQEEQAMTVDSRQAMLLLGAMGIAAVIIGMLAALIIS
                     RLIVAPLQQTVVFAQRIAAGDLTHDLPQDRRDEPGQLMAAMQVMSVSLRTLVGRIGGG
                     VSQIAAAAEQLSAITAQTSAGVQNQKMETEQTATAMHEMAATVQEVAQNAEQASLAAR
                     AADQEAQQGNLVVQQAVSQIDHLATEVEESAEAIAALSLESARIGGVLEVIRNVAEQT
                     NLLALNAAIEAARAGEQGRGFAVVADEVRALAKRAQDSTEEIEGLIASLQRLAKGAVE
                     KMDSSRSLTQRTVTLAGEAGDALGRITQAVSTIEQMNQQIAAAAEEQSAVAETISESV
                     TRVRDIGEQSASASEQTAASSAELARLGVELQGLVRQFRT"
BASE COUNT         1601 a         2535 c         2300 g         1463 t
ORIGIN      
        1 cgcgcccgct ccggttactt cgtccgtgag ctggcgcggc attccctggc cgagccagaa
       61 accagcccgc aattgatgca aaccaccgag gtggatgtca gcgagctggt gttttcggta
      121 ctcgcttcgc taaaagaccc aggcaccgtg ccattcggct cggccttccc cagcccgcaa
      181 ctatttcccc tgccacgcct ggcacgctct atggcacaca gcctgcgcga tatgcagccg
      241 caggcggtga ttgccgacat gaccgagggc aaccccaacc tgcgccggca gattgccctg
      301 cgttatatgg tcagcggcgt gatgctgccg ctggaggaac tggtgatcag cagcggcgcc
      361 atggaagcgc tcaacctgtg cctgcaatgc gtaactcagc cgggcgacct ggtagcaatc
      421 gaggcaccgg ccttctatgc ctgtctacag gtgttggaac gcttgcaact caaagccgtg
      481 gaaatcccgg tcaacccgcg tgaaggtatt gatctggatg tgctgagcga acgcctggcc
      541 ggcctgccga tcagggcctg ctggtttatg agcagcctgc agaatccgct cggcgcgagc
      601 atgagcgatg acaagaaggc ctcgctgtac caactcctgc agcgccacca ggtgccgctg
      661 attgaggatg acgtctacgc cgagctgtac ttcggcagcc agccgccgaa gccggtcaag
      721 agcttcgacc gcgacggcct ggtgatgcac tgtggctcgt tttccaaatg cctggccccc
      781 ggttatcggg tcggctgggt ggccggcggg cgttatgccg agcagatcag tcggctcaag
      841 ttgatgacca ccatctcgcc ctcggtgccg gcccaggccg ccctggccga ttatctgcag
      901 cacggcggtt atgaccgcca cctgcgcaaa ctgcgccatg ctctggaggc gcaacaagcg
      961 tcgatgctcg cctcggcggc gcggcacttc ccggccagca ccaaggtcac ccggcccagc
     1021 ggcggctatt ttctttggtt cgagtttccc gaacaggtcg atgcgctgcg cctgttccaa
     1081 ctggccctgg cccagggcat cagcctggct ccgggaccga tcttctcggc cacccgacgc
     1141 ttcggcaact gcgcccgctt gaattacggc cacccctgga acgccgaaag tgaacaggct
     1201 atggcgacat tggggcgtat tctcgcctcg ttctgagcca tacaacgtgc tgcttatcgg
     1261 ctgtgcataa cgcctctttc agcccatcct gtacaactcc cgtataaagc tgacggtcat
     1321 tgactgctct gctggatagg ccgtgctcgt cttttaaaca gagcaagcaa cggctatttg
     1381 cgggcattac ggccaattta tggacttcta tgcagcacaa ataccggcca tcggccacta
     1441 tcgctaaaac acaaaacaaa attgtacaaa gcaatatttc tgtataagat ttgcacaaga
     1501 agacatttaa taccttgctg agcctcccct tgaaacgcag caagcagtcg cagccccaca
     1561 gggcaagagg agtttatttc atgcaatcac aactgagcat cgcgcaacgc ctgagccttg
     1621 gatttggctt aattttgtcg ctgatgatcc tgattaccct catcggcgtg cagcgcgttg
     1681 gatttatcga cgagaccctg acctcggtga gtgaaggggc ttcgcttaag cagcgttatg
     1741 ccatcaactt ccgtggcagc gtgcatgacc gtgcgatcgc catccgcgat gcagtgctgg
     1801 tggataatga cgctgcgttg aatcgccagt tggcagaaat tcagcgactc aaggatttct
     1861 accaagagtc ggcccaggcc atgagcacct tgctgcgtga gcaagcggca acggataccg
     1921 agcaacgcct gctgagtgat attcagacga tcgagcgcag cactctgacc ttgactgaac
     1981 aactgatcga cctgcgccgc cagggtgata cagacgcggc gaaaagcctg ctactggaac
     2041 aagcttcacc tgcttatagc gaatggctca agcgcatcaa tgccttcatc gagcatgagg
     2101 aacaaggtgt cgtccaggat attagcgcgg tgcgcgaggc cgctagcggc ttccgtctgc
     2161 tgattctgct ggtcaccgct gccgctgtgc tgctgagcat tggcgtgtcg ctgattatta
     2221 tcaacaagtt gaaatccacc ctcggcgccg agccccatga agtggccgac gtcatccagc
     2281 gcctggccaa aggtgaactc aaccagcgta tccagacccg ctacccggat agtgtcatgg
     2341 gtgccctgca agatatggtc aagcacctgg ccgagaccat cacccaggtg cgcagtgccg
     2401 ccggtgaact gaccctggcc tcagaccagt tgctgactac atccgacagt aataaccagc
     2461 agatccgcct gcaatcgagc gaggccgaac agatggcaac cgccatcaac cagatggccg
     2521 ccaccgtcaa tgaagtggcc aactatgccg ccagtgcggc ctccgccact cgcaacgcgg
     2581 accatgaagt ggccaccggc aatcaagttg tcagcgctac cgccagcgcc atccagaacc
     2641 tggcgcgcac cctggaaagc gccgccgaaa gcgtgcagca ggtgtccacc gacagcgaga
     2701 gcattgaaaa gatcatcgaa gtgattacct cgattgccga gcaaaccaac ctgctggccc
     2761 ttaacgccgc catcgaagct gcgcgggccg gcgaacatgg ccgtggcttt gccgtggtgg
     2821 ccgacgaggt gcgttcgctg gccacccgca cccaggactc gactcgggaa attcgcggca
     2881 tgatcggcaa actgcaggaa ggtgccggca aagcggccga ggtgatgcgc aacagtcgtc
     2941 aactagccca gcagacagtc gagcaaaccg gccaggcgga aacggcgctg aacaagatcc
     3001 gcagcgaagt caccgccatc aacgaaatga acgcgcagat cgccagcgcc tcggaagaac
     3061 agagcgcggt ggccgaagag gttaaccaga acatcaaccg catccacagc gccaccctgg
     3121 aaacctcctc cggctccgaa caggttgctg cctccagccg cgaactggcg accctggcca
     3181 atcgtctgac tgacaaggtc agctttttca agctctagca ggctgtagca ggcatgccca
     3241 tgaaaccttt gcttgaacat tagccgctgc caaaacctgg gcgttcggct aatttagccg
     3301 cctgtttaat tcagagatca gtcatggact cgataaccca ggcggtactt ggcgccacga
     3361 ttcagggcgc actgctcggc cgctggcagg ggcgcaaggc gctgctgtac ggcgccatgc
     3421 tcggcacgtt gccggatctg gatgtggtca tcgattacgg cgacgccgtg gccgatatga
     3481 cctaccaccg tggtttcagc cattcgctgt ttgttctcac ggggctggca ttgctactca
     3541 cctggctgac ccgacgattc aggcacaacc ccggttactc agcgcagcgg ctatttatca
     3601 ccctgtggct ggtgctgatt acccatccac tactcgacag cttcaccagt tacggcaccc
     3661 agctactctg gccgctgacg ccaattccca ccgcctggtc gagcatcttt atcatcgacc
     3721 cgctgtacac cgtaccgctg tgcatcgccg tagcactggg gctgctttat ggcctgcgcg
     3781 ataaggcagc caaggctccg gccctggccc tgctggtttc atccctttat ctgggcttta
     3841 ccctggccgg caaacacatg gccgagcagc gcgtggaagc ggaactggcg cgtcagggca
     3901 tcgaggcgca gcaaattttc agcaccacca caccgttcaa cagcctgctg tggcgggtga
     3961 tcgtgcttga tggtgaggat taccacgaag cgctggtcag ttggtttgac aatacaccgc
     4021 cgacgcttgc gcgcatcccc cgtggtacgc acctggccgc agctctggtc gattcgacgg
     4081 cgcatgcgcg gctggcctgg tttaccaatg gtgtgctgcg ctacgaccag ctgggcgagc
     4141 agttggtggt gaccgacatc cgactgggca tgaccggctt ccaccctttc cgcttcgact
     4201 tcgccaccct ggaaaacggc cagtggcgcg tgcatgacga catccaacgt ctgccgatgg
     4261 accgcggcga tctctcgcgc ctgcagctgt tgtgggcgcg catctggcag cccaaggtac
     4321 aagtaccgct gctggtttgg gccgatgaac tgcgtaaagc gccgctggcc agtagcacgc
     4381 aataacagcg cttgccggcc cgcagagacg ccacgcatag gctaggctga attccaacct
     4441 ttacccgttg gagtctggcc atgaatatcc ggcagaaaat gatcgcctgt ggtgcgatca
     4501 gcgtactggc tgccaccctg ctcggtagca tcggtttctg ggggcaaacc cgcctggcca
     4561 gtgccctcaa cgaaaaccag ctgagcgtca gcgccctgcg caaccacctg gaaggcgaca
     4621 tgatgcacga cgccctacgc gccgatgtac tcgccgcctt ttatatcgac cccaccgaca
     4681 gcgcggcggt aacccaggta cgcgatgacc tgcgcgacca cagcgaatgg tttacccgca
     4741 ccctcaacga caacgccaaa ctgccgctca gcgcggatat tcgccaggcc atcagccagt
     4801 cgcagccggc cctggccaga tacattcagc aggccgaggg gatcgtcaat ctggccctga
     4861 ctgacccgca ggccgcacgc caacagatgg cagccttcga tgccagcttc ggcgatctgg
     4921 aagaaaagaa cgaagccctc agcggcctga tcgaagcgca ctcggcgcaa acccgcagcc
     4981 aaggcgaagc cgccgtgcaa caagccgcct ggctgctgat tggcggcatt ctggtggtct
     5041 gctgcctgct ctgcctgctc actacccagt tgatgaaagc ggtattgcac ccactggaga
     5101 aaatcatcga ggtggcgcgc aacattgccc agggcaacct gcgcagcacc atcagcgtcg
     5161 acagcaacga tgaagccggg caactgcagc aggcactggc ggatatgcag ggcaatctgc
     5221 ggcaaatgat cgacggcatc cgtcaggaag gtgaacagct gcagcaaacc gcgcgcaacc
     5281 tcaacggtgc ctcgcagagc atcgtgcgca gcaccacgga agaatcgcag agcgccagca
     5341 gcatggccgg ggccatggaa cagatgatgc agaacatcga acagatcgcc ggccacgcgc
     5401 gcaatgccca ggaaatctcc tcgcactccg aacacctggc cagcaatggc ggccaggtga
     5461 tcatgggcgt ggtcgatggc atgagccgga ttgccgaagc ggtcaacgaa tccagcgcca
     5521 ccatcaccgc gctgggtgaa tcatccgaag aaattcactc gatcattcaa gtgatcaaga
     5581 gcattgcgga gcaaaccaac ttacttgcgc taaacgccgc cattgaggcc gcgcgcgccg
     5641 gtgaagccgg ccgtggtttt gccgtggtgg ccgacgaagt acgtaacctg gctgcacgca
     5701 ccgcgcaatc gacccaggaa atcaccggga tgatcgaacg catccgcacc agcaccgaac
     5761 aggcagttaa cagcatgcag acaggggtca cccgggtgaa cgatggcgtg accctggcac
     5821 gccaggccgg tgagtcgatc agtgaaatcc gcggcggcgc acaacgcgcg gccgcggtgg
     5881 tggaagagat atcgcagacc atcgacgaac agagccgcgc cagcagcgag gtggcgcagc
     5941 aagtcgaaca tattgcgcag atgtcgcagc aaaacagccg cacggtaaat gaactggccg
     6001 acgccgcgca aagcctcgac cgcgtggcgc gcagtatgca aagcgccgtg ctgcagtttc
     6061 agacctgagc caaacggatc aggtgcggaa ttgccgcacc agcccttgca gctccacacc
     6121 caggcgcgcc agttcggcgc ttgaggcggc agtttgctcg ctagcactgg cgctttgctc
     6181 gccgatgtcg cgtacccgag tcacgctttc gctaatagtc tcggccaccg cgctctgctc
     6241 ttcggcggcg gcggcgatct gctggttcat ctgctcgatg gtgcttaccg cctgggtaat
     6301 gcggcccagg gcatcaccag cttcacccgc cagggtgacg gtgcgctgag tcaggctgcg
     6361 gctgctgtcc attttctcca ccgcgccctt ggccaggcgt tgcagactgg caatcagccc
     6421 ttcgatttct tcggtggaat cctgcgcacg cttggccagg gcgcgcactt catcggccac
     6481 cacggcaaaa ccacggccct gctcgccggc gcgggcggcc tcgatggcgg cgttgagtgc
     6541 gagtagattg gtctgttcag caacgttgcg aatcacctcc agcacgccac caatccgcgc
     6601 gctttccaga ctcagagcgg cgatggcttc ggcggattcc tccacttcgg tggccaggtg
     6661 gtcgatctga ctgaccgcct gttgcaccac cagattgccc tgctgcgcct cctgatcggc
     6721 agcccgcgca gccagggaag cctgctcggc gttctgcgcc acttcctgca cggtcgcagc
     6781 catttcatgc atggcggtgg cggtctgttc ggtctccatc ttctggttct gcacgccggc
     6841 gctggtttgc gcggtgatcg ccgacagctg ctcggcagcg gcggcaatct ggctcacgcc
     6901 accgccaata cgcccgacca gggtgcgcag gctcacgctc atcacctgca tggcggccat
     6961 caattgaccg ggttcgtcac gtcgatcctg cggcaggtcg tgggtcaggt cgccagcggc
     7021 aatgcgctgg gcaaacacca cggtctgctg caagggcgcg acaatcagac gggaaataat
     7081 caacgctgcc agcatgccaa taatcaccgc agcaatgccc atggcaccga gcagcagcat
     7141 ggcctggcgg ctgtccacgg tcatggcctg ttcttcctgg gctttggctt cctccaccag
     7201 ggccagcacc gcttgggcac gctcgaccat ggcggctgcg ctctgctggc tctgctcacg
     7261 catctgctgg tagttagcga acgcctgctg gtaaagggtt agcgccttca aggcggtttc
     7321 gatggcgcct ttctgactgt cgtccagcca gaccatcagg ctgcgcgcca cggtttgcag
     7381 gtcatcgctg atataggtcc actcctccag ggcttcggcc gaaccatcga taatgtactg
     7441 gctttcatgg ccacgcaggt cgagcatgcg cttgctcagg cccgaggcgg tttccgccag
     7501 agtcagaggg tcgctgccgc gcagcttgtc gcccgcgagg cgcaactcac gcaccgcgtc
     7561 gtacatgtcc agctcaatca cgtcgaactg atcacgcgcc tcgccggccg cctcacgcat
     7621 ttgcgtgcgc gccgcgcggg cattgttctg ctgctcgaca aaactgtcga actggcgacg
     7681 atagtcgtcg atggcctgct gcatgctttt cagacgcgcc tgatcagcag tggaagcggc
     7741 tgccagctgc tgggcgactg cggaactgac acgatccagc tgctcatgta cctgggcggc
     7801 cgcctcgctg gtctgccgca aggcgaaatc acgctcggcg cggcgcgcct gcaacacctg
     7861 ggcatcgatt gccgacaggc cactggtgct ggcatgccc
//
LOCUS       DASRUM010000005         2610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1173907, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000005 DASRUM010000000
VERSION     DASRUM010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173907"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..1557)
                     /gene="tilS"
                     /locus_tag="VFF22_00290"
     CDS             complement(241..1557)
                     /gene="tilS"
                     /locus_tag="VFF22_00290"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008057329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZX15294.1"
                     /translation="MTPVHIRLRDALQPWRSTAGWCVALSGGLDSTVLLHLLASLAQR
                     EALPPLSAIHIHHGLQAAADVWPAHCRELCAALCVPLQVEYVQVAPGASLERAARDAR
                     YAAFAARLGAGELLLTAQHRDDQAETLLFRLLRGAGVQGLSAMPLSRALGAGQLVRPL
                     LNCSRDELLAYAREHKLTWIEDPSNADERFSRNYLRRQVVPALLSRWPQAATNMARSA
                     AHLSEAAQLLDELAQQDMAAAQVSAEFAWLPLPNLALPALRGLSEPRQRNALRYWLRP
                     LTAMPDSEHWAGWQALRDSASDAAPIWKLAAGELRRSDERLWWLAGDWLCSPVPVNVT
                     LQGNQQVALPANGTVRIDGQLPSGNWQLGYRQGGDQILLPGRGHRDLKRLFNELRVPA
                     FVRDRLPLLRLDNQVMAIANLPGMQGAVDGSWQLHWQPPTSDQGLS"
     gene            complement(1618..2568)
                     /locus_tag="VFF22_00295"
     CDS             complement(1618..2568)
                     /locus_tag="VFF22_00295"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HZX15295.1"
                     /translation="MNPNFLDFEQPIADLQAKIEELRLVGNDNSLNIGDEIARLQDKS
                     SSLTESIFGNLSSWQIAQLARHPRRPYTLDYLQHIFTEFDELHGDRHFSDDAALVGGV
                     ARLDGEPVMVIGHQKGREIREKVRRNFGMPRPEGYRKACRLMEMAERFKMPILTFIDT
                     PGAYPGIDAEERNQSEAIAWNLRVMARLKTPIIATVIGEGGSGGALAIGVCDQLNMLQ
                     YSTYSVISPEGCASILWKTAEKAPDAAEAMGITAERLKGLGIVDQVIGEPLGGAHSDP
                     AAAAESVRQALLTQLAMLKTFDTDALLKRRYERLMSYGIA"
BASE COUNT          591 a          813 c          751 g          455 t
ORIGIN      
        1 caatgaagcc gaggcgatgc ctttccccaa tgaagaaaca acaccacccg tgacgaatat
       61 gtagcgcgtc atgaaaaacc ctagaagtct gcgtttaagc ggtcaatgcc gccggggaaa
      121 gcgaaggaat accgaagcgg ctctctgcga tgcactgcaa tgagctagcc ctatcgaccc
      181 ccaaagggag tcaacaaaag ctgtagtaag acgggagcgt agtctaccgg aaaggcctta
      241 tcagctcaaa ccttgatcac tcgtcggtgg ctgccaatgc aattgccagc tgccatcaac
      301 agcaccttgc atgcctggca ggttggctat ggccataacc tggttatcca gacgcagcaa
      361 tggcaggcga tcacggacaa aagccggcac gcgcagctca ttaaacaagc gcttgagatc
      421 gcggtgtccg cgtccaggta aaagaatctg atcaccgccc tggcgatagc ccaactgcca
      481 gttacctgaa ggtaactgac cgtcaattcg cacagtgcca ttagcgggca atgccacctg
      541 ctgattacct tgaagcgtca cattgaccgg cacgggcgag cacagccaat cacccgccaa
      601 ccaccataga cgctcatcac tgcggcgcaa ctcccccgcc gccagcttcc agattggcgc
      661 agcatccgac gcggaatcgc gcaacgcctg ccaaccggcc cagtgctcac tatcgggcat
      721 cgccgtcagc ggacgcagcc agtaacgcag agcattgcgc tgacgtggct cacttaggcc
      781 gcgcagcgca ggcagcgcca gatttggtaa cggcaaccag gcaaactccg ctgacacctg
      841 cgcggcagcc atatcctgct gcgccaactc atcgagcaac tgtgcagcct cactcaagtg
      901 cgcggcgcta cgggccatat ttgtggcggc ctgcggccag cgactgagca gcgcaggcac
      961 cacttgacga cgcaggtaat tgcgcgagaa gcgctcatcg gcatttgacg gatcttcgat
     1021 ccaggtcagc ttatgctcac gggcataagc caacagttca tcgcgcgagc agttgagtag
     1081 cggccgaacc agctgccccg ctcccaatgc acgacttagc ggcatggccg acaaaccctg
     1141 cacgccggca ccgcgcaaca ggcgaaacaa cagagtctca gcctggtcat cgcgatgctg
     1201 cgcagtcagc agcagttcac ctgcgccaag acgagccgca aaagccgcgt aacgagcatc
     1261 gcgcgcagca cgctcaagac tggcgcctgg agcaacttga acatattcaa cctgcagcgg
     1321 cacgcacagg gccgcgcaca gctcacggca atgcgccggc cagacatcgg ccgcagcctg
     1381 caggccatgg tggatatgaa tagcggaaag aggcggcaat gcctcgcgtt gcgcgaggct
     1441 ggccagcagg tgcagcagca ccgtggagtc cagcccgccg gacagggcaa cacaccagcc
     1501 tgcggtacta cgccagggct gcagggcatc acgcaaacga atatgcacag gagtcatcgg
     1561 ccaagcttaa aacaacaacg ggcccgaagg cccgttgtat gcgcaactgc caagcaatca
     1621 ggcaatcccg tagctcatca ggcgttcata tcgacgcttg agcagcgcgt cggtatcgaa
     1681 ggtcttgagc attgccagct gcgtcagcaa agcctgacgc accgattcgg cggcagcagc
     1741 tgggtcgctg tgtgcgccgc caagcggctc accaatcacc tgatcaacaa tgcccaggcc
     1801 ttttaggcgc tcggcagtaa tgcccatcgc ctcggcggca tccggcgctt tttccgcggt
     1861 tttccacaga atagaggcgc aaccttcggg cgagatcacc gaataggtgg agtactgcag
     1921 catgttcagc tgatcgcaca caccaatcgc caaggcaccg ccggaaccac cctcaccgat
     1981 gacggtggcg atgatcgggg ttttcaggcg tgccattaca cgcaggttcc aggcgatggc
     2041 ttcgctctgg ttgcgctctt cggcgtcgat gcctgggtag gcgcccggag tgtcgataaa
     2101 ggtcaggatc ggcatcttga agcgttcggc catttccatc aggcggcacg ccttgcggta
     2161 gccttcgggg cgcggcatgc cgaagttgcg gcgcaccttt tcgcgaatct cacggccttt
     2221 ctggtgaccg ataaccatca ccggctcacc atccagacgt gcaacgccgc ccaccagggc
     2281 tgcgtcgtcg gagaagtgac gatcgccatg cagctcatcg aactcggtaa agatgtgctg
     2341 caggtaatcc agggtataag gacggcgcgg atggcgcgcc aactgggcga tctgccagct
     2401 gctcaggttg ccgaaaatgc tttcggtcag cgagctgctc ttgtcctgca ggcgggcgat
     2461 ttcatcgccg atgttcaacg agttgtcgtt accgaccagg cgaagctctt cgatcttggc
     2521 ttgcaggtcg gcgatcggct gttcgaaatc gagaaaattc gggttcatag tcatccgtct
     2581 tgcgtcgacg gccaagtggc cgggagctgt
//
LOCUS       DASRUM010000006        44015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1340852, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000006 DASRUM010000000
VERSION     DASRUM010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 44015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44015
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340852"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VFF22_00300"
     CDS             complement(<1..939)
                     /locus_tag="VFF22_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZX15296.1"
                     /translation="MASKILLVEDDRALREALADTLALGGHDYRAVDCAEAALLAIAE
                     EPFGLVVSDVNMPGMDGHQLLAQIRQRQPQLPVLLMTAFGAVERAVDAMRQGAADYLV
                     KPFEPKALLTLVDRHALGRVSVSEQDGPIAEEPASVQLLALATRVAQSDSTVLITGES
                     GTGKEVLARFIHQQSPRGSKPFIAINCAAIPDNMLEATLFGHEKGAFTGAIASAPGKF
                     ELAEGGTILLDEISEMPLGLQAKLLRVLQEREVERVGARKPITLDIRVLATSNRDLAA
                     EVAAGRFREDLYYRLSVFPLAWQPLRERPGDIVPLAE"
     gene            complement(946..2154)
                     /locus_tag="VFF22_00305"
     CDS             complement(946..2154)
                     /locus_tag="VFF22_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX15297.1"
                     /translation="MNANAQRPEESEAPAPVEQASRAGLEQAFALFNQMSTQLSTSYS
                     MLEARVTDLKGQLALVSAQRMQELAEKERLAHRLQSLLDLLPGGVIVIDGQGVVREAN
                     PVARNLLGQPLVGMLWRQVIARNFAPREDDGHEISLKDGRRLSIATRSLHAEPGQLVL
                     LTDLTETRRLQDQLSRHERLSALGRMVASLAHQIRTPLSAALLYASHLTEQVLPVEQQ
                     QRFAGRLKERLHELEHQVRDMLIFARGELPLPDRLAPKALFDALRSAAEPHVLDMQVR
                     WQCDSRAGELLCNRDTLVGTVLNLIENAIQAGGREARLKIHLYQRGETLRLCISDNGP
                     GIDSATLARLGEPFFTTKTTGTGLGLAVVKAVARAHQGDVQLRSRPGRGTCAILTLPL
                     LGATHPLIQE"
     gene            complement(2340..3812)
                     /locus_tag="VFF22_00310"
     CDS             complement(2340..3812)
                     /locus_tag="VFF22_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZX15298.1"
                     /translation="MWRETKILLIDDNSERRRDLTVILNFLGEDHLASDSQNWRQCVA
                     ELASSREVLSVLLGEVKAKGGVLDMLKQLAAWDEFLPTLLINDQVAAEWPEEQRRCVL
                     ATLEMPMSYNKLLDSLHRAQVYREMYDQARERGRQRETNLFRSLVGTSRAIQQVRQMM
                     QQVADTEASVLILGESGTGKEVVARNLHYHSKRREAPFVPVNCGAIPAELLESELFGH
                     EKGAFTGAITSRAGRFELANGGTLFLDEIGDMPLPMQVKLLRVLQERTFERVGSNKTQ
                     SADVRIIAATHKNLEKMIEDGSFREDLYYRLNVFPIEMAPLRERIEDIPLLMNELISR
                     MEFEKRGSIRFNSAAIMSLCRHDWAGNVRELANLVERMAIMHPYGVIGVGELPKKFRH
                     VDDEDETLVASLREDLDERATILAGLPGLDSMAMLPPEGLDLKDYLGNLEQGLIQQAL
                     DDASGVVARAAERLRIRRTTLVEKMRKYGMSRRDDEGLED"
     gene            complement(4053..4349)
                     /gene="fliT"
                     /locus_tag="VFF22_00315"
     CDS             complement(4053..4349)
                     /gene="fliT"
                     /locus_tag="VFF22_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003152328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HZX15299.1"
                     /translation="MSSSVQRLEETGSALRTALAIQDWAAIGELDLQCRQAVDEAMVE
                     SVSDEDTLRARMQELLDLYRELVNVCQAEQQRLANELVQLNQSQQGAKVYQLFG"
     gene            complement(4377..4757)
                     /gene="fliS"
                     /locus_tag="VFF22_00320"
     CDS             complement(4377..4757)
                     /gene="fliS"
                     /locus_tag="VFF22_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HZX15300.1"
                     /translation="MNAMAALRQYQHVNTHVQAVEATPHRLIQMLMEGGLTRLAQARG
                     AMERGQIALKGELIGKAVGIIGGLREGLNLELGGEVAANLDSLYEYMISRLVDANVQN
                     EIAPLDEVAGLLRNVKAGWDAIAE"
     gene            complement(4825..5211)
                     /gene="fliS"
                     /locus_tag="VFF22_00325"
     CDS             complement(4825..5211)
                     /gene="fliS"
                     /locus_tag="VFF22_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HZX15301.1"
                     /translation="MNKPIDTYKKVKTSQDVSQYRAVALLLDGAIERVKLARQAQIEG
                     NSEVRGLAVGTTITILGVLQASLDKQLGGEIAENLDVLYAYMTRRLAGAAIEREAGSL
                     EEVEALLAQLQEAWAGIEAEVEPSAS"
     gene            complement(5215..6597)
                     /gene="fliD"
                     /locus_tag="VFF22_00330"
     CDS             complement(5215..6597)
                     /gene="fliD"
                     /locus_tag="VFF22_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HZX15302.1"
                     /translation="MAGITGIGSGIDIDAIVKASVAAERAPKDAQLARLTTKTTTQIT
                     SLGSFKSALSEFQTAAKGLNDASLFGTRKASSSDATRLTASAEKTALTGTYRVEVEQL
                     ASASKIASGSLTGDSSLAFASGGKLTIGLGADSFDVDVAAGATLKEVRDAINTQLNEK
                     GISANIISDPANGTSQLVLSSNKTGAGNDLTLAASGADLQGLVTNMPAALSEAQNAKF
                     KIDGLSLESATNAVSGAIEGVTFTLVKAEEGVSTTLTVGENTTAVKDSLKKFVDAYNK
                     LITTTNSLTSFTKVGESGEPLTGALLGDSSVRNVLNGLRSELSNTAAGQSGDPFRALA
                     DLGITTQKDGKLAIDDTKLTAALEDNFDAVGSFITGDKGLMNRLSDRVDGYVKSGGVL
                     EQRISGLQKTDADIKEQKADLEIRVAAIQARLYSQYNAMDSLIGQLSRTSDSLGSALS
                     SLPGVVRKDS"
     gene            complement(6672..7064)
                     /locus_tag="VFF22_00335"
     CDS             complement(6672..7064)
                     /locus_tag="VFF22_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HZX15303.1"
                     /translation="MDIGSIRDSMKQGGLVAEPSKPRATDVADKGGALSAIADTAQTK
                     AAADKQQAEKPATRAELETAMSNIQEFVQSVRRDLNFSLDDGNGRVVVKVTDATSGDV
                     IRQIPSEEALKLAENLTEVRSLLFKAEA"
     gene            complement(7135..8325)
                     /locus_tag="VFF22_00340"
     CDS             complement(7135..8325)
                     /locus_tag="VFF22_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HZX15304.1"
                     /translation="MALTVNTNIASLNTQRNLNTSGKALDTSLQRLSTGSRINSAKDD
                     AAGLQISNRLSSQINGLNVAVKNSNDGISMAQTAEGALQQSTSILQRMRDLSLQSANG
                     SNSDEERKALNSEVTELKKELNRISNTTTFGGKKLLDGSFETTTFQVGSAANETISVK
                     IDEMSTESLEGTFSSGAIDAGDVATADAAASGAIAVSVTMVGGEARTFTATFASGATA
                     AEQVQALATVINDANIGVGAFVNDDGDIDLVTALGSGAETGQIESLSFGSGASVDAAK
                     TDATTNAITVDEVDDGNLVDDIDITDVIGSQKAVIVIDAAIQAIDAQRADLGAVQNRF
                     ENTIGNLQNISENVSAARGRIQDTDFAAETANLTKNQILQQAGTAILAQANQLPQAVL
                     SLLG"
     gene            complement(8591..13645)
                     /locus_tag="VFF22_00345"
     CDS             complement(8591..13645)
                     /locus_tag="VFF22_00345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014590146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZX15305.1"
                     /translation="MTGGRCLGEPWRVGIAGLACGDPEQVFVLSEADWQNLVLQLPWE
                     QLKAYGDSNGLIFEPVSRTDSDCYDGLLLVACPHEAQPLLAMNPQVRRYLLLRGEQAR
                     FSAEIEETFARIFAPAGEPFTNGHLVCLNQPLLVSGVYPEDQPLNEPRLLVCDDQPDF
                     PGWLSTKWRECLEAGHIPLHMGSWQVPDWVPADIYVDGHNFADQEKLDRHWKENGRKI
                     AGALNDFLVGKSSYPYSSDCWVTALTAAIAGDATAVREAAPLLSIIIPTYNHGRFLGQ
                     ALRSVLEQGYEDIEVLVLDNASTDGTEEVMRAFAAEPRVRYLRNQRNLGPSYNVLNGI
                     QIATGRYLSMLMADDFYNEGYLSRLLPLLLENPKVVVGYTSIRWVNEQGQALAMPRHP
                     GYREEDYIGGRNEVAELLTYDNYIPPSAALIRREPLLKVWKRDPQLNGAGDWLSMVQV
                     AEQYADFVFLSDPGVTYRRHAAQLSTAFYASAAPLADHIRIVEGVFERGAEGKLRGSE
                     RDVAAHLRRRLALYPSEQASELGERVRHLCERLEALARKDEEILFSIILTTYNRPDLL
                     KDALASVGSQTLRDFEVVLVNDNGEPVESLLAACDFPVTYVRQGCNRGLSAARNAGLK
                     LARGTYVCYLDDDDFYWPEHLATLARTFASHPNTVVYTGVEYVDERLEVGRRIELGRS
                     RPFLHQQYDRERLFIQNYIPVNTWAHPRSMLATVGEFDTGLTAFEDWDMLLRLGARYT
                     FVHAPEVTTEVRVRQGSTAGSDHMLGRERKNFGALYEEMYRRHADLGSERVRAGRRKL
                     LQQFGVKSGQPRQTLSLDAWLAQRTLTPLQQRLIDEHLSGQVPRIGVAILDLDGAAEQ
                     VVATYESLQGVLYPHVRPWLLAPVDYRDLPADLPIHLATKQDWPLVLNQLLQTNEFDW
                     LCIVQAGERFTPGGLLMAALELLGAPECRAVYCDELYQPATGGLGSLFRPALNLDYLL
                     SFPAGMARHWLFRRDVAVQAGGFDLQFIDAPEFELILRLINQDGLAGLGHVAEPLLIG
                     RAPQLVDHEHEQRAISQHLALRGYEQAQVLSSQPGRYRIRYGHAETPLVSILIEAGGR
                     LAPLQRCVESLLESTDYAQYEVLLIEDRTDAADVSTWLEALETLGEARVRVLRGYAGV
                     CVAERLNTAARLVSGSLLLMLHPETAAFEPEWLGEMVNHALRPEVGVVGGLLLSAAGK
                     VTQAALVLGLEGPVGEPFIGQPASAPGYMHRLEVTQNCSAVSRRLLMIKAPLFDELNG
                     VSEAGVAAGYLDVDLCLRARQAGYLIVWTPYARLLSERVDVPRPSVDEQDGFYARWLP
                     LLARDPAYNPNLSLAQPGGFKLADTAMSWRPLASFKPLPVVLAHPADQFGCGNYRVMQ
                     PFHAQQQAGLIDGVLSVGLMHVADLERYAPDVVLLQRQIGDERIDAMRRMKAFSPAFK
                     VYELDDYLPNLPLKNVHRQHMPKDILRSLRRGLAHADRFVVSTEPLAEAFAGLHPDIR
                     VMQNRLPVPWWQGLQSQRRMGTRPRVGWAGGSSHTGDLELIADVVRELANEVDWVFFG
                     MCPESMRPYIKEMHAGVEIGRYPAMLASLNLDLALAPVEDNLFNACKSNLRLLEYGAC
                     GFPVICSDVRCYQDGLPVTRVKNRFRDWVDAIRMHLADLDACAQAGDELRRTVQRDWM
                     LEGANLHAWRAAWLPD"
     gene            complement(13808..15112)
                     /locus_tag="VFF22_00350"
     CDS             complement(13808..15112)
                     /locus_tag="VFF22_00350"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HZX15306.1"
                     /translation="MLAPTAMKYGLITNVMTFGHLTAGSRSNLGDDIQTHAVEHLYAC
                     MGIAPEQIVRLNRYEFRNYDGRYGYILMPMCGYFTLGNAQSPLPMSPYIIPVYFSFGL
                     SSDVDDPADLEHFRRHEPIGTRDERVMHMFRERGISAFVSGCLTITFPRREYAPVDGK
                     VFLVDVPDELLEFVPAELADRVERLTHIYPYERIPSDQDEADRLDALARELCRRYADE
                     GQLVVTSRLHCAAPCIAMGIPTIVVAHNISDRFAWLDRYVKIHTRETFGEIDWQVGVP
                     DIEDSKQMIRSAAAKQIEAARARWAPLAEISGFYESRERSVYNNLMVQTLQRLLLDKE
                     KPLRFAFWGANTHGVRLTRALRELWPGSELVTVVDEYLQSDSFCGVPVVRSGVLDELR
                     REGIYVFICTFAGRDYAERHLLEKGVEHGCLFDDLLTFHALS"
     gene            complement(15124..16035)
                     /locus_tag="VFF22_00355"
     CDS             complement(15124..16035)
                     /locus_tag="VFF22_00355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HZX15307.1"
                     /translation="MTGEIDKFQYGEYINDQHAGLFDYPEFLAGTDVAEIRITAEGVS
                     VSSAAHDISMLVDSVDLHATPYTLLDFGSYETDETAFLKSVFRDGEVFLDIGANLGWY
                     SLVLGRKCPTSQIYAFEPIPTTVTVLEKNIRLNRLDNIETICMGMFDKEDELNFLYAP
                     DVSGATSLKLTGQSRGRTSIQNVVCRTTTLDSFCTTRNIVPSLLKIDVEGAELMVVQG
                     GTKTLESTPIILMELLRKWSRAFGYHPNDVFALLEEYGYRAWVFVDGGRLQPCPQVTE
                     ETVQTNYIFMHPQKHAEVISQWELPAR"
     gene            complement(16098..17162)
                     /locus_tag="VFF22_00360"
     CDS             complement(16098..17162)
                     /locus_tag="VFF22_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016488441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZX15308.1"
                     /translation="MNPPMGVPCRHPVIAEDLQLIFSRPEDWQRFAGSHVLIAGATSF
                     LAAYLVEFFASLSERWPERPVIIHALARDPEKLRRRFPHLVGQSWFRPIIQNVCDPVP
                     TLERVDFIVHAAGAGSPKQYLSDPVGTTRANVVGMLNLLELAKLHGARLLFMSSGAVY
                     GHGESDSPIGEYDFGVLDPLDVSACYGESKRMAETLCADYHRQYGVATVMARISHTYG
                     PGIALDDGRSFADFIADALAGRDIVLNSDGSGSRPFCYVSDTTAAFLILLLRGEGGNA
                     YNVGLDQETTILELAKLIVRLSPTPNLQVKHPPPNPERQALFRSSGHFNLSKMEGLGW
                     RPSILPEAGFARTLRYFIQS"
     gene            complement(17164..18033)
                     /locus_tag="VFF22_00365"
     CDS             complement(17164..18033)
                     /locus_tag="VFF22_00365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX15309.1"
                     /translation="MVSRELIARHCICCGSTQLNRSPAVLMPFVAQRVFGHEPVEITA
                     EWGLRDLRQGMAYTLCNSLQCQECGALFLDFRFTDEQMAALYHNYRDEHYNAQRIRYE
                     PGYVNVVPHYHQRAAYLSEVEAWLAPHLPDTPAVLDWGGDTGINSLFRECNRLLHIHD
                     ISGVELVPGAERVDLGLIGQSQYDLVTCSQVLEHVPFPLELVQRMLPALKSGTLLYLE
                     VPHEALVREHPDSLELAALKRHWHEHINFYTETAVRRLLGSLSLTVVDVLRLPVDVGW
                     SRGEVMGVLARLD"
     gene            complement(18027..18857)
                     /locus_tag="VFF22_00370"
     CDS             complement(18027..18857)
                     /locus_tag="VFF22_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX15310.1"
                     /translation="MASMESLFRLDGRVAVVTGGAGLLGYQHAATIAGLGGVPVLLDI
                     NTAGLASNVARLTEESGCEAQALVADITDLDAVLRAFEQLMARHGRVDILINNAARNP
                     KVESAGDKDFSRLEHFPWEQWQLDLNVGLGGAFNCAKVFGAQMARQGTGVIVNIASDL
                     GVIAPDQRLYRVEGLAPDQQPVKPVTYSVVKHGLIGLTKYLATYWCEQGVRCNALSPG
                     GVYAGQNDVFVSQLSQLIPLGRMAEADEYRGAIAFLCSDASSYMNGANLVVDGGRSIW
                     "
     gene            complement(18850..19902)
                     /locus_tag="VFF22_00375"
     CDS             complement(18850..19902)
                     /locus_tag="VFF22_00375"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZX15311.1"
                     /translation="MKVLFCGLGSIGQRHLRNLRQLLGDELQVHAYRVRGQRIKLKEG
                     GVEEGADLERDYGVVVHESLAQALASNPDAAFICNPNSLHIPVALACAHAGVAIFMEK
                     PLASELEGIAELDALVREKNLLFHVGYNYRFHPGLQRIKTLVDEGFFGRLLTARGEIG
                     EHLPDWHRYEDYRQMYASRAVQGGGVIRSQIHEMDLICWLFGLPLSIVTHGGQLSSLE
                     IDVEDTASSLMRCSGEAGSFPILLHQDFLQRPAVRTFKVVGDCGVAEIDLLANRLRVF
                     DAQGALCEQDDFPGFVRNDMYLAQARHFLDCLGSGAAPLVSLRDGVQSLRLALAALRS
                     LNEGREVALNEVNIHG"
     gene            complement(19899..20786)
                     /locus_tag="VFF22_00380"
     CDS             complement(19899..20786)
                     /locus_tag="VFF22_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZX15312.1"
                     /translation="MPADYLATYAAAGVYTKQGRLGPQLDGHYQSFPRTTWEAELQTL
                     GSTPSIRGLEWIYDLYGEGANPLETAAGRRHMNDCLVRSGIELVSVCADYFMDCPLVR
                     CSEAQRLERCERLIWLIGICPEVGIRHIVLPLLDTSSIKTPQEREIVVACLRQVLPLA
                     QESGVRLNLESDLPPQGFRELLLEIDHPMVGVNYDTGNSAAAGFDPRDEFKAYGSWIN
                     SVHVKDRRRNAGTVPLGEGDTDFPLLRELLRQIDYQGSFVMEAARGESGQEPAWQARM
                     AARTVNWLSGGMMTDEVRV"
     gene            complement(20789..22579)
                     /locus_tag="VFF22_00385"
     CDS             complement(20789..22579)
                     /locus_tag="VFF22_00385"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZX15313.1"
                     /translation="MRVADYIFEFIANQGVGHVFFLPGGGAMHLNNALLRQPRLSAVS
                     MLHEQGAAIAAEGYARTSGKFGACLVTSGPGATNAITGLAGAWFESTPVMFISGQVKR
                     ADLKGSSGLRQLGTQELDIVSVVQPLSKYAVCLLDPLRVRYELEKALHLMLGGRKGPV
                     WLDVPLDVQATEVDPEQLQGYTPDVMDQPPVLDIQALQHLGQRLVEAQRPVLLVGNGV
                     HGAAAEAGMRKLVEQLGIPTLTTWIGADLLEYEHPLYFGRCGTVAPRGANFTVQNADL
                     VLAIGCRMDFSITGFNRSHFARQAEVIVVDIDPAEIAKLGDMPDESFVCDAGVFIDGL
                     SSCMPTERLQLDDWLARCTEWKTRYPVVLPEYREQRGFVNTYVFTETLCDALDEGELI
                     IPGSSGAAIDTFWISAKLKRGQRTVATGGLGSMGYGLPAALGGCLGAGSKRTISVDGD
                     GGFVMNIQELEVVRRLQLPIKYFVLNNNGYASIRASQTGYFKETIGCDPASGMSLPNI
                     VALAGAFGVRSVQVSDQAALSATIREVLDYPGPVVCEVLVEPDQPIGPRVTSRIGDNG
                     VMVSSPLEDLYPFLDRGELEANMLVPLVQE"
     gene            complement(22584..23780)
                     /locus_tag="VFF22_00390"
     CDS             complement(22584..23780)
                     /locus_tag="VFF22_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HZX15314.1"
                     /translation="MSTETLESRLKSLLNEPLTVVRERQSDTLTRLIGGFERPFALFG
                     ASHLGRKVQTVLKGMGLTPCAFMDNNPALWGTHIDSVPVCSPAQLAEQWHGDLPAVIC
                     TIWSGHIHDRMADRLQPLRELGFEHIALFGHLAWRYPEHFLPHYSMDLPEKVLGEVDA
                     IQYAFSLLGDEASRRLFVDHVAWRLHLDHDLLPGSSPLPIYFDPHFSTDFSGEVVFDL
                     GAFNGDTLEGYLASGRSFSAIHCFEPVASNFTALQARLERLAQPGVYAHRLAVGDSQG
                     EVLIEAANGPSSRVGFGDEQVTMTTLDDLVAQGMLPTFIKIDIEGFEPQCLAGGRRLI
                     SECQPVIAVSVYHEQNHLWRILLQLHSYHDGYRFSLCPHVSDGWDLVLYAVPAGRVPL
                     NSCCEP"
     gene            complement(23777..24988)
                     /locus_tag="VFF22_00395"
     CDS             complement(23777..24988)
                     /locus_tag="VFF22_00395"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HZX15315.1"
                     /translation="MNLTSRGDAESRLESLLSESLASVRQRQRETLGLIMGGEQAPFV
                     LFGAGGLGRQVLPVLRAAGLCPLAFIDNNPALWGSNIDGIEVLGPAQLAERMAGCLPA
                     VVASIGAVRVGTSHLEGWRAPLRSLGFQRIALFGHLAWRFPQLLPYQCLDLPEQVLAE
                     ADRVREAFHLLADEDSRNLYVAHVAWRLTLDSDLLPAESGEAIYFSDRFSASDDAEVL
                     YDIGAFNGDSILGYLESGRRYREIHSFEPSEGNFDDLQKTISGLVESGHLGVQAHLLA
                     VGDHAGSIAIGVEGGHTLKVGEGDGMVSMTTLDLLAEHLTPPSFIKMDIEGFEPQCLA
                     GARRVIAEKQPALAVCAYHEQDHLWRLLLQVHSYHAGYHFMLGQHLYEKAWDIVLYAI
                     PESRLPKGTQS"
     gene            complement(24985..26889)
                     /locus_tag="VFF22_00400"
     CDS             complement(24985..26889)
                     /locus_tag="VFF22_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014590137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HZX15316.1"
                     /translation="MTSILIDGVPLGGDRVFVIAEAGNNHNGSLELARQLVDAALAAG
                     ADCVKFQLRNRDALYRRKADGSRAEDLGVEYIQDLLDKVELSLDAHRQLRAYCAQKGI
                     IYLCTPWDEPSVDALADFDVAALKIASADLCNPYLIAKAASLGKPLILSTGMSFEHEI
                     VRAIEQLNTLGTPFALLHCNSAYPAPEVDIQLGYLRRLQELHDLVGYSGHERGIAVTL
                     GAVALGAKLVERHITLDRDMEGPDHLASLEPEEFRQLVEGIRQLELALPWQGPGRHAS
                     QGELLNRENLGKSVIAACNIARGTVLDASMLRVASPGQGLPPYRLPELLGKTATRDIA
                     QSDFLFDSDLDEQQAQQSLAFALPIRWGVPVRYHDFSDYRRRIEPDLYEFHLSYRDLS
                     LQPQPFLSEVACSRLVVHAPELFENSELLDLVADDPAYRQRSIDNLQRVVDATLQIGE
                     FFPRADARLIVANIGGFSADEPRPLAMRAQLYERFHDACSRIDFAGTELIPQNMAPFP
                     WHFGGQRHQNIFMMPDELAAQAREHGLRLCLDLSHLQMTCFHFNLDFQAALALLLPYS
                     AHLHVADAKGSNGEGVLMGTGDIDWPATWAQLIRHPEVSFIPEVWQGHKDHGAGFWAA
                     LRFLSELEAR"
     gene            complement(26886..28250)
                     /locus_tag="VFF22_00405"
     CDS             complement(26886..28250)
                     /locus_tag="VFF22_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019471412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZX15317.1"
                     /translation="MSSLVKALTIDMQQTILDAIKCIESGNLQIAFVVAEDMRLLGVV
                     TNGDIRRYLLQGGLTAQPVTACMNREFRSVSIATPREDLLKAFDLGHNALPGLDAEGR
                     LVEVYTRQMAASAEVPVLARARAPVRMSFCGGGADLTYFFIDHPAAVLSCTVGLYAHA
                     TLVPRDDRQIHIFSEDLDREEHFDSLPTLLAAAEKSLLATIVSVIKPKYGFDLYLRSD
                     FPVGSGLGGSSAATIASIAAFNELRQDRWNTYEIAELAFQAERLCFGIAGGWQDQYAS
                     AFGGFNLIEFENQRNLVHPIRLEEAIRNELESCLVLCDTGISHDSGRLHELQREEIAA
                     ESSQTELLSASVALCRRMHRYLMRGELRDFGLCLDEAWQLKKRFSSAISHSRLDEIYE
                     AALAAGALGGKLLGAGGGGFFLFYVQPQHRQRVVQTARALGCRTQNFRFESCGVTSWR
                     TKIQ"
     gene            complement(28250..28942)
                     /locus_tag="VFF22_00410"
     CDS             complement(28250..28942)
                     /locus_tag="VFF22_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZX15318.1"
                     /translation="MHAYILCGGFGTRLRTVIKDTQKAVVDIHGRPFLALVLRQLREA
                     GVSRGVLCAHYRADQLAALLPSLEAESGLALQLVVEETPLGTGGAVLNALLQVPPGGR
                     YLVINADTFLDSSAYRLASAAENEVLVGVQVEDRSRYGSLCCSVQGEILGFEEKGQVG
                     SGWVSAGVYGFSADALAGMPITSCSMEHDLLPSLIRQHRLYICEYRGAFVDIGTPDSL
                     NAFKSDLLGSAV"
     gene            complement(28942..30267)
                     /gene="rfbH"
                     /locus_tag="VFF22_00415"
     CDS             complement(28942..30267)
                     /gene="rfbH"
                     /locus_tag="VFF22_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HZX15319.1"
                     /translation="MSDSTAQQLRQQIAELVGRYAELQMAPKPFVAGQSLVPPSGKVI
                     GAGELQSMTEAVLDGWLTTGRFNAAFEKRLAKYLGRRCVLTTNSGSSANLLAFSALTS
                     PRLGERAIKPGDEVIGVAAGFPTTVNPILQFGAVPVFVDVELPTYNIDPAKIEAAISP
                     KTKAIMLAHTLGNPFDLSVVRALCDKYGLWLIEDCCDALGSIYQGRMVGTFGDIGTLS
                     FYPAHHITMGEGGAVFTNNPALKRIIESFRDWGRDCYCAPGEDNTCGKRFCWQLGDLP
                     EGYDHKYTYSHLGYNLKITDMQAACALAQMDRLDDFILARKRNFAWLSERLAGCRDFL
                     ILPQATPGSDPSWFGYPMTLRDGCGIRRVELLRYLDAQGVGTRLLFAGNLVRQPYMQG
                     RNFRVSEQLPITDKVMNDTFWIGVWPGLAEEHLEHAARCLETFFGLRVG"
     gene            complement(30264..31328)
                     /gene="rfbG"
                     /locus_tag="VFF22_00420"
     CDS             complement(30264..31328)
                     /gene="rfbG"
                     /locus_tag="VFF22_00420"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HZX15320.1"
                     /translation="MALSPAFWKGRKVLLTGHTGFKGGWLAIWLRQLGVDLRGFALPA
                     ATTPSLWQVAHLESQVAGDLADIRDADRLAVAMQAFQPELVLHLAAQPLVRESYRAPA
                     ETYATNVIGTLNLLEAVRATASVRAVLVVTTDKCYENREWSWPYREQDALGGFDPYSS
                     SKACVELLCASYRDSFLRASGVALATARAGNVLGGGDWSPERLLPDIFRAWQEGREVV
                     LRYPHATRPWQHVLDPLMGYLQLAQGLLNDPEHHAAAWNFGPDSAGLATVEAVVGRLA
                     EHWPGEARWSVSADAQPHEAGLLALDSSRARQSLGWVPRWSLEATLKHTLDWQLAWQE
                     GRDMTVVTREQIAVHQGEIQ"
     gene            complement(31319..32089)
                     /gene="rfbF"
                     /locus_tag="VFF22_00425"
     CDS             complement(31319..32089)
                     /gene="rfbF"
                     /locus_tag="VFF22_00425"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016488448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HZX15321.1"
                     /translation="MKVVILAGGLGTRISEESHLKPKPMIEIGGKPIIWHIMKIYSHY
                     GINDFVICLGYKGYVIKEYFANYFLHMSDVTFDMAENRMHIHQRHAEPWRVTLVDTGE
                     ATSTGGRLKRVREYLGDETFCFTYGDGVSNIDIPALIAFHKQHGKLATVTAVQPPGRY
                     GALDVQGQMVSGFQEKPLGDGGWINGGFFVLEPAVIDYIDGDATTWEFEPMRTLASEG
                     QLMSHLHRGFWQPMDTLRDRVLLEEHWRSGEAEWQLWR"
     gene            complement(32407..33654)
                     /gene="flgL"
                     /locus_tag="VFF22_00430"
     CDS             complement(32407..33654)
                     /gene="flgL"
                     /locus_tag="VFF22_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HZX15322.1"
                     /translation="MRISTIQAFNNGVTGLQRNYADVTRTQEQISTGNRILTPADDPV
                     ASVRLLQLEQQQSVLGQYNENLAAANSSLTQEEVTLNSVNTVLQRVRELTVQAGNGAL
                     SKEDRESIAAELGEREEELFSLVNTRNARGEYLFSGFQGKTQAFVRDGDGSYSYQGDE
                     GQRKLQIASSLQVPISDSGKAIFENIVNAGRLDGALDLTGAPGSTLRASAPLVQDEVA
                     FGSTPPFPEAGVEVIFGNPDANSYEVFEIGAAAPAPVLASGTLDDDPDTRDQMVFRGV
                     SVLFDGTALGGESLQVTVDPTRQKQGILDTIATLRRTLEDPASTDAQIRDSVAIGLTN
                     LDNGMINVDSVRGAIGARLNVIDTTLTDNEDVALVNKGIQADLRELDYPEALSRLSFQ
                     TIILEASQQSYVKIASLNLFNKL"
     gene            complement(33737..35779)
                     /gene="flgK"
                     /locus_tag="VFF22_00435"
     CDS             complement(33737..35779)
                     /gene="flgK"
                     /locus_tag="VFF22_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HZX15323.1"
                     /translation="MADLLNIGLSGLSANKTSLAVTGHNITNVNTPGYSRQDAVQATR
                     VPQFSGVGYIGSGTTLVDIRRTYSEFLTAQVRSSTALSADVEAYKAQIEQLDSLLAGT
                     TTGITPSLQSFFSALQTAAEDPANIPARQLVLSEAEGLAKRFNTVYDRVSEQNEFINK
                     QMSAVTEQVNRLATSIASLNEAIAVAAANGKQPNDLLDAREEAVRQLSTYIGVSVVAQ
                     DDSSLNVFVGSGQPLVVGSTASRLEAVAGRDDPTRFEIEFVSGGSRQGVTSLLTGGEL
                     GGLIRYRSETLDPTLNSIGRLALAVADQVNSQLGQGLDLKGQVGAALFGDFNDPATAQ
                     LRVRASAGNTSNVQPALNITDSSVLTTSDYRLEFDGTQYSARRLSDGATLTVNPNPPG
                     SYPAALSFTDASGRDQGFEIALDAAPAANDRYLLQPTRRGATAIEAVLDQPDQLAFAA
                     PVRADATVQNRGTATIGQPTLQSTGSNIDPAALQAALLPPVTLTYDAGTSDFTVPAGA
                     TLTRINPNDGTPATPATFQSGQQNTYELELSDGSRMRMTLSGRPENGDTFSLSFNQNG
                     VSDNRNALALADLQSKATVGLDPTITGISTGSSFVDSYGDTVERVGTLTAQARQDSLA
                     TTSILKQATDNRDSLSGVSLDEEAANLIKFEQYYNASAQVIQVARSLFDTLIGAFR"
     gene            complement(35786..36958)
                     /gene="flgJ"
                     /locus_tag="VFF22_00440"
     CDS             complement(35786..36958)
                     /gene="flgJ"
                     /locus_tag="VFF22_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HZX15324.1"
                     /translation="MDSRLSAGFLGGTSTVDSGAYNDLNRLNQFKVGGDSEKNIKKVA
                     QEFESLFLNEMLKAMRSANEAFGEGNFMNSNESKTYQDMHDQQLSVTLSNHQNGIGLA
                     DVLVRQMSKMKQADSRPNPFAQVNEPVPSAPSKPLAKVDSARDDTSLINQRRLSLPGK
                     LTDRLLAGIVPSATAADGQPLAQNDWIPAKSFAAPADTALGLNGMDAITGRRIAQPPL
                     APGKSAFGSKDEFIAAMLPMAEQAAEKIGVDPRYLVAQAALETGWGKSIIRQQDGTSS
                     HNLFGIKTHNTWEGDSARVLTTEFKGGKAVKEAASFRAYDSFEHSFNDFVSFLQGNGR
                     YEKALASTDKPEQFARELQKAGYATDPQYARKIAQIARQMQTYQTVAAVSTPSTNG"
     gene            complement(36972..38075)
                     /locus_tag="VFF22_00445"
     CDS             complement(36972..38075)
                     /locus_tag="VFF22_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HZX15325.1"
                     /translation="MTLSKYLLAAAALLLASLAQAERLKDLASIQGVRTNQLIGYGLV
                     VGLNGSGDQTTQTPFTVQTFNNMMAQFGIKVPQGGNVQLKNVAAVSIHADLPAFAKPG
                     QTIDITVSSIGNAKSLRGGSLLMTPLKGIDGNVYAIAQGNLVVGGFDAEGGDGSRITV
                     NVPSAGRIPGGATVERPVPSGFDQGNTLTLNLNRPDFTTAKNIVDHINELLGPGVAQA
                     VDGGSIRVSAPLDPGQRVDYLSILENLEVEIGQAVAKVIINSRTGTIVIGQNVRVQPA
                     AVTHGSLTVTITEDPIVSQPEALSGGQTAVVPRSRVNADEEAKPMFKFGPGTTLDEIV
                     RAVNQVGASPSDLMAILEALKQAGALQADLIVI"
     gene            complement(38087..38806)
                     /gene="flgH"
                     /locus_tag="VFF22_00450"
     CDS             complement(38087..38806)
                     /gene="flgH"
                     /locus_tag="VFF22_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HZX15326.1"
                     /translation="MNRLIIISALLGPLALSGCMAPAPKPDDPYYAPVMPRTPLPAAQ
                     NNGSIYQAGFENGLFGDRKAYRVGDIITITLNERTQASKNSNSQISKDSSANLGVPNL
                     FGMTVAPKNPLAGLSALGMTNDTLGLDASFDATRSADGSGAAGQSNSLTGSITVSVAE
                     VMPNGVLLVRGEKWMTLNTGDELVRIAGLVRADDIATDNTVSSTRIADARITYSGTGA
                     FADASQPGWMSRFFISPMWPF"
     gene            complement(38927..39712)
                     /gene="flgG"
                     /locus_tag="VFF22_00455"
     CDS             complement(38927..39712)
                     /gene="flgG"
                     /locus_tag="VFF22_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HZX15327.1"
                     /translation="MLPALWVSKTGLSAQDMNLTTISNNLANVSTTGFKRDRAEFEDL
                     LYQIRRQPGGQSSQDSQLPSGLQLGTGVRVVGTQKIFTAGSLQTTEQPLDMAINGRGF
                     FQVLLPDGTVSYSRDGSFHLNSDGQVVTSNGYALEPAIVLPNEVRTFTVGEDGTVSVT
                     TAGNPQPQIVGNLQTADFINPAGLEAIGNNLFLETASSGAPQVGTPGLNGLGTTLQNT
                     LENSNVSVVEELVNMITTQRAYEMNSKVISTADQMLGFISQNL"
     gene            complement(39759..40499)
                     /locus_tag="VFF22_00460"
     CDS             complement(39759..40499)
                     /locus_tag="VFF22_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HZX15328.1"
                     /translation="MDKMLYVSMTGAHNNTLAQRAHANNLANITTNGFRRDFEQARSM
                     PIFGDSYPSRVYAMSERPGTDFTPGSLQETGRDLDVAVGGKGWLAVQAPDGSEAYVRT
                     ASLNVDSLGVLRTGNGLPVMGNAGPIAVPPEQKIEIGKDGTISIRALGEAPNVLAEVD
                     RLKLVNPDLKQMEKGTDGMIRFKGQGPVQADANMEITSGFLESSNVNAVEEMTAILSL
                     SRQFELQVKMMRTAEDNSSAMARVLQMS"
     gene            complement(40705..42051)
                     /gene="flgE"
                     /locus_tag="VFF22_00465"
     CDS             complement(40705..42051)
                     /gene="flgE"
                     /locus_tag="VFF22_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HZX15329.1"
                     /translation="MAFNIGLSGLRAATNDLNVTGNNIANAGTAGFKQSRAEFADVYA
                     SSVLGTGSNPQGSGVLLGDVSQLFNQGNINYTQNALDLAINGNGFFQVNNNGALSYTR
                     AGYFGTDREGFIVDNFGYNLQGYTTDANGVLQQGVVGNLQVQTASQEPRATTSISQGF
                     NLNSTLASPTNTPFDPGDPTTYNSSTSTNIYDTQGNAHVLTQYFVKDDANQWQMNILI
                     DGRSPSDPAAAAPVGTEPDRYQLNFDASGQISSLAQITDITTIPPTLGAAITPAGQFT
                     IGTWNPAAPDTNSPTGWSLNGAESNPGGFTFDMRGSTQFSSAFAVNRVSQDGYTTGEL
                     AGLEIDETGVIFARYTNGQSKVQGQVILSSFANVQGLTPVGKTQWVQSFESGEPVRNP
                     PGSGTLGSLQAGALEDSNVELSDQLVNLIVAQRNYQANAKTIETESAVTQTIINLR"
     gene            complement(42094..42762)
                     /gene="flgD"
                     /locus_tag="VFF22_00470"
     CDS             complement(42094..42762)
                     /gene="flgD"
                     /locus_tag="VFF22_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HZX15330.1"
                     /translation="MSTVSGTSSVLDQYQMQQGSTAQNKELGKNEFLNLLVAQLNNQN
                     PLEPQGNGEFIAQLAQFSQVEGIEKLNTSMSSLLTGYQSSQALQASSLVGRKVIVPTD
                     KAVVDTSESFKASLVLPTSSSNVYVNVYDSAGAVVSRVNLGEQKAGNVSFIWDGKDSG
                     GNLMPPGTYKFEAQATYDGEAKGLYTLLPANVDSVVLGGSELMLNLAGLGSVPLSQVQ
                     VIGQ"
     gene            complement(42782..43225)
                     /gene="flgC"
                     /locus_tag="VFF22_00475"
     CDS             complement(42782..43225)
                     /gene="flgC"
                     /locus_tag="VFF22_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HZX15331.1"
                     /translation="MSLASVFNIAGTGMSAQSTRLNTIASNIANAETVSSSIDQTYRA
                     RHPVFATMFQSAQNGDRGSLFAEQDEAGVGVQVLGVVEDQSSLMPRYEPNHPAADAGG
                     YVYYPNVNVVEEMADMISASRAFQTNTEMMNTAKQMMQRVLTLGQ"
     gene            complement(43241..43648)
                     /gene="flgB"
                     /locus_tag="VFF22_00480"
     CDS             complement(43241..43648)
                     /gene="flgB"
                     /locus_tag="VFF22_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008029381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HZX15332.1"
                     /translation="MSISFDRALGIHEQALGFRAQRAEVLANNIANADTPNYKARDLQ
                     FATVLAEQSAKSQRGQVSLNRTDSQHIPADGVIVGDASLAYRTPFHPSIDQNTVDLQI
                     EQSNYAENSVQFQASFTLLNSKFKGLVSALRGD"
BASE COUNT         9792 a        13372 c        12073 g         8778 t
ORIGIN      
        1 ctcggccagc ggcacaatgt cccccgggcg ctcacgcaat ggctgccagg ccagcggaaa
       61 taccgacaag cgatagtaga gatcttcacg gaaacgcccc gccgccactt ctgcggccag
      121 atcgcggtta ctggtggcca gcacgcggat atccaaggta atcggcttac gcgcacccac
      181 ccgctctacc tcgcgctctt gcagcacgcg cagcagcttg gcctgcagac ccagtggcat
      241 ctcggaaatc tcgtcgagca gaatggtgcc gccctcggcc agttcgaact tgcccggagc
      301 actggcaatt gccccggtaa atgcgccctt ctcatggccg aacagcgtgg cttcgagcat
      361 gttgtccgga atcgccgcgc aattaatggc aataaagggc ttgctgccgc gtggcgactg
      421 ctggtggata aagcgcgcca gcacctcttt acctgtgccg gattcgcccg tgatcaatac
      481 cgtcgaatcg ctctgcgcca cccgcgtggc cagggccagc agctgcacgc tggccggttc
      541 ctcggcaatc gggccatcct gttcgctgac agaaacccgg cccaatgcat gccggtcaac
      601 cagcgtcagc aaggctttcg gctcgaatgg tttgaccagg taatccgccg cgccttggcg
      661 catggcatcc actgcccgct ctaccgcgcc aaaggcggtc atcagcagca ccggcagttg
      721 tggctggcgc tgacgaatct gcgccagcaa ctggtggcca tccatgccgg gcatgtttac
      781 gtcgctgacc accaggccaa acggctcttc ggcaatcgcc aacagcgccg cttcggcgca
      841 atccaccgcg cggtaatcat gcccacccag cgccagggta tcggccagtg cctcgcgcaa
      901 tgcgcggtca tcttcgacca acagaatttt gctcgccatg gatggttact cctgaatcaa
      961 agggtgcgta gcaccaagca gcggcaaggt gagaatggcg caggtgccac gtcccggccg
     1021 cgaacgtagc tgcacatcgc cctgatgggc gcgcgccact gccttgacca cggccaggcc
     1081 aagcccggtg ccggtggttt tggtggtaaa gaagggttcg ccaagccgcg ccagcgtcgc
     1141 gctgtcgatg ccaggaccgt tgtcgctgat gcacaggcgc agcgtttcgc cacgctgata
     1201 cagatgaatc ttcaagcgcg cctcacggcc gccggcctga atcgcattct cgatcagatt
     1261 aagcacagtg ccgaccaggg tgtcgcggtt acacagcagc tcccccgcgc ggctgtcgca
     1321 ctgccagcgc acctgcatat ccagcacatg gggttcggcg gcgctgcgca atgcgtcgaa
     1381 caacgccttc ggcgccaaac gatccggcag cggcagctcg ccacgggcaa agatcagcat
     1441 gtcgcgcacc tgatgctcca gctcgtgcag acgctctttc aggcggccgg caaagcgctg
     1501 ctgttgctca actggcagca cctgctcggt caggtgactg gcatagagca gcgcagcgga
     1561 aagtggcgtg cgtatctgat gagccagaga agcaaccatg cggcccaaag cagacaaacg
     1621 ctcatgacgg gacaattgat cctgcaagcg ccgggtttct gtcagatcgg ttagtagcac
     1681 cagctgacca ggttcggcat gcaaggaacg ggtggcaatc gacaagcgcc ggccatcctt
     1741 cagcgagatt tcgtgaccat catcctcacg cggggcaaag ttgcgcgcga tcacctggcg
     1801 ccagagcatg cccacgagag gctgcccaag cagattgcgc gcaaccgggt tggcctcacg
     1861 caccacgccc tgaccatcga tcacgatcac cccgccaggc agcagatcca gcaggctctg
     1921 caggcggtga gccaagcgct ctttctcggc cagctcctgc atccgctgag cgctgaccag
     1981 cgccaactgg cccttaagat cagttacccg cgcctccagc atgctgtagc tggtgctgag
     2041 ctgagtcgac atctggttaa acaaggcaaa ggcctgttcg agcccggcac ggctggcttg
     2101 ctccacggga gcgggcgctt cggattcttc agggcgttgg gcgttagcgt tcatggttct
     2161 ctctcgcgca gcgagccgtc ataagacagt cagttgcaag agagatagca atacccgtgc
     2221 ctgaaaaaat acccttttaa atcaacgcca taaaaaacaa agccgaaggt tacgtcaaac
     2281 cttcggcttt attggaagga caagcgccaa gtcactgacg attgcctgac gcagcaaggt
     2341 cagtcttcca gcccctcatc atcacggcgg ctcatgccgt acttgcgcat tttctcaacc
     2401 agggtggtgc gtcgaatacg caagcgctcg gcggcacgcg ccacaacacc actggcatca
     2461 tccaacgcct gctgaatcag gccttgctca aggttgccta ggtaatcctt caggtccaat
     2521 ccctcaggcg gcagcatggc catcgaatcc aagcccggca aaccggccag gatggtggcg
     2581 cgctcatcca agtcctcacg caggctggca accaaggtct cgtcttcatc atcgacatgg
     2641 cggaatttct tcggcaactc gcccacgcca ataacgccat agggatgcat gatcgccata
     2701 cgctcgacca ggttggccaa ctcacgcacg ttacccgccc agtcatgccg gcacagcgac
     2761 atgatcgccg ccgagttgaa gcggatagaa cctcgcttct cgaactccat gcgcgagatc
     2821 agctcattca tcagcagcgg aatatcctcg atgcgctcac gcagcggggc catttcgatc
     2881 gggaagacgt tgaggcggta atacagatcc tcacggaagc tgccgtcctc gatcatcttt
     2941 tcgaggttct tgtgcgtggc agcaatgatc cgcacatcgg cgctctgggt cttgttgctg
     3001 cccacccgtt cgaacgtgcg ctcctgcaat acacgcagca acttgacctg catcggcaac
     3061 ggcatatcgc cgatttcatc aaggaacagg gtgccgccat tggccagttc gaaacgtcca
     3121 gcacggctgg tgatcgcacc ggtaaacgcg cccttctcgt ggccgaacaa ttcgctctcc
     3181 agcaactcgg caggaatcgc accgcagtta accggtacaa aaggcgcttc acgacgcttg
     3241 gagtggtaat gcaggttgcg cgcgaccact tccttgccag tgccagattc gccaagaatc
     3301 agcacgctgg cctcggtatc ggcaacctgc tgcatcatct gccgcacctg ctgaatggcc
     3361 cggctggtgc cgaccagact gcggaacaga ttggtctcgc gctgacgacc acgctcacgg
     3421 gcctgatcgt acatctcgcg gtaaacctgg gcgcggtgga gagagtcgag cagcttgttg
     3481 tagctcatcg gcatttccag cgtcgccagc acgcagcggc gctgttcttc cggccactca
     3541 gcagccacct gatcgttaat cagcagcgtt ggtaaaaact cgtcccaggc agccagctgt
     3601 ttgagcatgt ccagcacgcc acctttggct ttcacctcgc caagcaacac gctgagcact
     3661 tcacggctgg acgccaactc cgcgacgcat tgacgccagt tctggctatc actggccaga
     3721 tgatcttcac cgagaaaatt caagatcacc gtcaagtcac gacggcgctc gctgttatcg
     3781 tcaatcagga ggattttggt ttcacgccac ataatttatt tactgatcct ggagtgaaaa
     3841 acgcgcccgc cgtccaaata ccactcgatc gcgccggcaa ttggccagta gttaagtcaa
     3901 ttttatgtta atagtcaatt ttatggcgtg cttttattga gcccgcgaag cagacgaaat
     3961 ggtctggtca acatagaaag ggacagatcg gggcagaaag cagagatata ccagaaggtc
     4021 gataccggac agcaccgacc gagaataact aatcaaccga acagctgata gaccttagcg
     4081 ccttgttgcg actgattgag ctgaacgagc tcgttagcca agcgttgttg ctcggcttgg
     4141 caaacattaa ccagctcacg ataaagatcc agcaactcct gcatacgtgc gcgcagggtg
     4201 tcttcatcgc tgacagactc gaccatggct tcgtccacgg cctgacggca ttgcaaatcc
     4261 aactcgccaa tggctgccca atcctggata gccaaagcag tacgcaaagc gctgccggtt
     4321 tcttctagac gctgcacgga tgaactcata aacaattcct caggcgggaa acggcatcac
     4381 tcggcgatgg catcccagcc agcttttaca ttgcggagca ggcctgcaac ttcatccagc
     4441 ggcgctattt cattctgcac gttggcatca accaaacgcg agatcatgta ctcgtacaag
     4501 ctatccaaat tggcggccac ttcgcctccc aactccagat tcagaccttc gcgcaagcca
     4561 ccgataatgc cgacagcttt gccaatcagc tcacccttaa gggcaatttg accgcgctcc
     4621 atggcgcctc gggcctgggc caaccgtgtc agaccgccct ccataagcat ttgaatcaga
     4681 cggtgcggag tcgcctcaac ggcctgtacg tgagtattca catgctggta ttggcgcagg
     4741 gctgccattg cgttcatcgt catgtctcgc tgttgggctg cagatacttc tatatcgacg
     4801 cccagccaga aaactttaac gtattcagct ggcagatggc tctacttcgg cctctatccc
     4861 agcccaagct tcctggagtt gagccagcaa agcttcaacc tcttcgagac tgcccgcttc
     4921 gcgctcaata gccgcgccag ccaagcgacg agtcatgtag gcatagagta cgtcaagatt
     4981 ttcagcaatt tctccaccga gctgcttgtc caggctggcc tgcaatacac ccagaatagt
     5041 gatggtggtg cccactgcaa gcccacgcac ctcactgtta ccctcgattt gtgcctgacg
     5101 tgccagtttg acccgctcga tagcaccgtc gagcaacagt gcaacggcac ggtactgcga
     5161 gacatcctgg ctggttttga ctttcttgta ggtatcaatt ggcttattca tggatcaact
     5221 atcctttctc accacgcccg gcaggctgga caatgcgctg cccaatgaat cactggttcg
     5281 ggacaattgc ccgatcaagc tatccatggc gttatattgc gaatagaggc gcgcctgaat
     5341 cgcggccaca cgaatttcca gatcggcttt ctgttctttg atgtcagcat cagttttttg
     5401 taggccgctg atgcgctgct ctaaaacacc accactctta acataaccat caacacgatc
     5461 actgaggcga ttcatcaggc ccttatcacc agtgataaat gacccaaccg catcgaagtt
     5521 atcctcaaga gccgcggtga gcttggtatc gtcgatggcc aacttgccgt ctttttgtgt
     5581 ggtaatgccc agatcggcca gcgcacggaa cggatcgccg ctttgccctg ccgccgtatt
     5641 gctcagctca gaacgcaaac cgttaagcac attacgcacc gacgagtcgc caagcagggc
     5701 acctgtcaac ggttcgccac tctcaccaac cttagtaaag ctggtcaggc tattggtcgt
     5761 tgtgatgagc ttgttgtagg cgtcaacaaa cttcttcagg ctgtccttga ccgccgtcgt
     5821 gttctcgccc acggtcaggg tcgtactcac cccttcctca gctttgacca atgtgaaggt
     5881 cacaccctcg atcgctccac tgaccgcatt ggtggcgctc tccagggaca gaccatcgat
     5941 cttgaacttg gcgttctgcg cctcgctaag tgcagccggc atattggtca ccagcccttg
     6001 caggtcggca cccgacgccg ccagggtcag atcattacca gcgccggtct tgttggacga
     6061 gagcaccaac tgtgaggtgc cattagccgg gtcgctgatg atgttggcac tgatgccctt
     6121 ttcattgagc tgggtgttga tagcgtcgcg gacttccttg agcgttgcgc ccgcagcgac
     6181 gtcgacatca aaactgtcgg cacccaggcc gatagtcagc ttaccgccag aggcaaaggc
     6241 aaggctcgaa tccccagtga gactgccaga agcaatcttg gacgcactgg caagctgctc
     6301 gacctcgact cgataagtcc cggtaagtgc agttttttcg gcactggcag taagacgggt
     6361 cgcgtcagag gacgaggcct ttctggtacc gaacaggctg gcatcattca ggcccttggc
     6421 cgctgtctga aattcactca gggcactttt gaaagagccc aatgaggtaa tctgcgtggt
     6481 tgtcttggtt gtcaaccgcg ccagctgtgc gtctttaggc gcacgttctg ccgccactga
     6541 tgccttgacg atcgcgtcga tgtcgatccc cgatcctata cccgtgatac cagccatgtt
     6601 agctacctcg ccaaagggtt gacaccctat tcctgttacc taagcggtaa acaagtttca
     6661 tgccaccgcg ctcatgcctc agccttgaac agcaagctgc gaacctccgt gaggttttcg
     6721 gccagtttca gcgcttcttc cgagggaatc tgacggatca catcccccga ggtggcatca
     6781 gtcactttca ccacgacgcg gccattgcca tcgtcgaggg aaaaattgag atcgcgccgc
     6841 acggactgca cgaattcctg aatattgctc atggccgttt ccagctcagc ccgggtagcc
     6901 ggcttttcag cctgctgctt atcggctgcc gccttggtct gcgccgtatc ggcaatcgcc
     6961 gatagcgccc cacctttatc agccacatcc gttgcgcggg gcttgctggg ctctgccacc
     7021 aaaccgcctt gcttcatcga atcacgtatc gaaccaatgt ccatagtctt ttcctcaaaa
     7081 agagggaatg gggaggcagg ttaccctggc tccccatcga tgctaccccg gagcttagcc
     7141 cagcaggctg agcactgcct gcggcaactg gttggcctgg gccaggatcg ccgtacctgc
     7201 ctgctgcagg atctggttct tggtcaggtt agccgtttcc gctgcgaagt cagtatcctg
     7261 aattcgaccg cgggctgccg atacgttttc cgagatgttc tgcaggttac cgatggtgtt
     7321 ctcgaagcgg ttctgtaccg caccaagatc agctcgctga gcatcgatcg cctggatggc
     7381 agcgtcgatt acgattaccg ccttctgcga accaatcacg tcggtgatat cgatgtcgtc
     7441 gaccagattg ccgtcatcta cttcatccac ggtgatggcg ttagtagtag catctgtttt
     7501 agcagcatct accgaagccc ccgagccgaa gctcagcgat tcaatttggc cagtctctgc
     7561 gccggaaccc aacgccgtta ccaggtcaat atcgccatcg tcattgacga acgcaccgac
     7621 accaatgttg gcatcgttga tcacggtcgc cagagcctgc acctgctcag ccgcagtcgc
     7681 accgctggcg aaggtcgcgg taaaggtacg cgcttcacca ccgaccatgg tgaccgatac
     7741 agcgatggcg ccggaggctg ccgcatcagc agtagcaaca tcaccagcat caatggcacc
     7801 cgagctgaaa gtaccctcca gcgactcggt gctcatttcg tcgattttga cgctgatggt
     7861 ttcgttcgca gcactaccaa cctggaaggt ggtggtctcg aaggagccgt cgagcagttt
     7921 cttgccaccg aaggtggtgg tgttggaaat acggttcagc tctttcttca gctcggtcac
     7981 ttccgagttc agggctttac gttcttcatc gctgttcgaa ccgttggccg actgcaggga
     8041 cagatcacgc atacgctgca gaatgctggt ggactgctga agtgcgcctt cagcggtctg
     8101 cgccatcgag atgccgtcgt tggagttctt caccgcaacg ttaaggccat tgatctggct
     8161 actcagacgg ttggagatct gcaggccggc tgcatcgtcc ttggcgctgt tgatacgcga
     8221 accggtggac agacgctgca gggaggtgtc cagggccttg ccagaggtat tcaggttgcg
     8281 ctgagtgttg agggacgcaa tgttcgtgtt gactgtaaga gccatgatgt atgtcctcca
     8341 tcggacctag cttagttgcc tgaggctgcg aaacccggtc ctgcaattct caggcagcca
     8401 ttgagttcgc gttcgtatta cttatcggct tgtgtggcag gagctttagg gtggagtgaa
     8461 aatttttttc tttccgcatt gacagcgcta ccgcccacct aactcgcatc agttctatcg
     8521 gcacgttcgg cctcagcttt agccattcct gtaaatgcat caggccgctc gaaagcggcc
     8581 tgatgggagg tcaatcagga agccatgccg ctcgccaggc atgtaggttc gccccttcaa
     8641 gcatccagtc gcgctgtact gtcctgcgca actcatcacc cgcctgggcg caggcgtcga
     8701 ggtcggcaag gtgcatgcgg atggcgtcaa cccaatcgcg gaaacggttc ttcacccgcg
     8761 taacgggcaa cccatcctga taacaacgca catcactgca gatgacagga aaaccgcagg
     8821 caccatactc gagcaagcgc aggttgcttt tgcaggcgtt gaacagatta tcttcaaccg
     8881 gtgccagagc gaggtccagg ttgagcgatg ccagcatggc cgggtagcgt ccaatctcga
     8941 caccagcatg catttccttg atatagggac gcatgctctc gggacacata ccgaaaaaca
     9001 cccagtccac ttcattggcc aactcgcgca ccacatcagc tatcagttcc agatcgccgg
     9061 tatgactgga gccgccggcc catccaacgc gtggtcgcgt gcccatcctc cgctgactct
     9121 gcagcccctg ccaccagggc acgggtaagc ggttctgcat aacccggata tccgggtgca
     9181 ggcctgcaaa agcctcggcc agcggctctg tggaaaccac aaaacggtcg gcatgggcca
     9241 gaccacgacg caacgagcgc aaaatatctt taggcatatg ctgccggtgg acatttttta
     9301 gcggcagatt cggcaggtaa tcgtctagct catagacctt gaatgcgggg gaaaaagcct
     9361 tcatccgccg cattgcatcg atgcgctcgt caccgatctg ccgctgcagc agcacgacat
     9421 cgggggcata gcgttccaga tcagcaacgt gcatgagccc caccgacagc acgccatcaa
     9481 tcaagcccgc ctgctgctgc gcatggaatg gctgcatgac acgataattc ccgcagccaa
     9541 actggtccgc agggtgagcc aacactaccg gtaatggttt aaacgaggcc aaagggcgcc
     9601 aggacattgc cgtatcggca agcttgaagc ctccaggctg tgccagtgaa aggttcgggt
     9661 tgtaggcagg gtcacgcgcc agcagtggta accaacgagc ataaaaccca tcctgctcat
     9721 cgacacttgg acgaggtaca tctacacgtt cagataacaa ccgtgcataa ggcgtccaca
     9781 caatcagata gcctgcctga cgagcacgca gacagaggtc aacgtccaag taaccggccg
     9841 ctacccccgc ctccgacaca ccattcagct catcgaacaa cggtgccttg atcatcaaaa
     9901 gacgacggct gaccgcacta caattttgtg taacttccag ccgatgcata taacccggag
     9961 ctgaagcagg ctgaccgata aaaggctctc ccaccggccc ttccagcccc aacaccagag
    10021 cagcctgagt gaccttaccc gccgccgata gcagcagccc tcccacaact cccacttccg
    10081 ggcgcagtgc atgattgacc atctcgccca accactcggg ctcgaaggcg gccgtttccg
    10141 gatgaagcat cagcaacagg ctacccgaga ccaagcgagc agccgtattc aggcgttcag
    10201 ccacgcagac acccgcgtac ccacgcagga cccgaacacg cgcctctcct agcgtctcaa
    10261 gcgcttcaag ccaggtggag acatcggctg catcagtacg gtcctcaatc aagagaactt
    10321 cgtactgtgc atagtccgta ctttccagca ggctttccac gcaccgctgc agcggcgcca
    10381 agcggccgcc ggcctctatc agaatcgaga ccaagggggt ttcggcatga ccgtaacgaa
    10441 ttcgatatcg gccaggttgt gatgacaaaa cttgcgcttg ctcatagccg cgtaaggcca
    10501 agtgctggct aattgcacgc tgctcatgct catgatcgac aagctgtggg gctcgcccga
    10561 tcaacagcgg ctcggccaca tgccctaacc ccgccaaacc atcctgattg atcaagcgaa
    10621 ggatcagctc gaactccgga gcatcgataa attgcagatc gaagccccca gcctgtaccg
    10681 ccacatcgcg gcggaacaac caatgacgcg ccattccagc cgggaagctg agcaggtagt
    10741 caaggttcag tgctgggcgg aacagacttc ccaggccgcc cgtagccggc tgatacagtt
    10801 catcgcaata aaccgcacgg cattctggcg cccccaatag ctccaacgcc gccatcaaca
    10861 agccgccggg tgtaaagcgc tccccagcct ggacgatgca cagccagtcg aattcgttgg
    10921 tttgcaacaa ttggttgagt accaacggcc aatcttgttt ggtcgctagg tgaataggta
    10981 agtcagcagg caagtcgcga tagtctacag gtgcgagcaa ccagggacgg acatgcggat
    11041 aaagcacccc ttgcagcgac tcgtaggtgg ccacaacctg ctccgccgca ccgtcaagat
    11101 cgagaatggc caccccgata cgcggtacct ggccacttag gtgctcatca atcaaccgct
    11161 gctgcagcgg tgtaagcgtc cgctgagcca gccaagcatc cagactcaag gtctgccgag
    11221 gctggccgct tttaacgcca aattgctgca acagcttgcg ccgccccgca cggacacgct
    11281 cactgccgag gtctgcatgg cggcggtaca tttcctcata gagcgcaccg aaattcttac
    11341 gctcacgccc cagcatgtgg tcactgccgg ctgtactgcc ctgccttacg cgcacttcgg
    11401 tcgttacctc aggcgcatgc acaaaggtat aacgtgcgcc cagacgcagg agcatgtccc
    11461 aatcctcgaa cgccgttagc cccgtatcga actcccccac agtcgccagc atgctgcgcg
    11521 ggtgagccca ggtattgacc ggaatgtagt tttggatgaa cagccgctcg cgatcatact
    11581 gctgatggag aaacggtcgg ctgcgtccaa gttcaatgcg acgccccacc tccaaacgct
    11641 cgtcaacata ctcaacgccg gtatacacca cagtattggg gtgactggca aaggtacgtg
    11701 ccagtgtggc caggtgttct ggccaataga agtcatcgtc atcgaggtag caaacatagg
    11761 tgccccgcgc gagcttcaag ccggcgttac gggccgccga caacccccgg ttgcacccct
    11821 gccgcacgta ggtgacagga aaatcacacg cggccaacaa agactccact ggctcgccgt
    11881 tgtcattgac caggacgacc tcaaaatcgc gcaaggtctg actaccgacg ctggccagag
    11941 cgtccttgag aagatcggga cggttgtaag tggtcaggat gatggagaac agaatctctt
    12001 catccttccg ggcgagagcc tcgaggcgtt cacacaaatg ccggacccgc tcccccagct
    12061 ccgacgcttg ctccgacgga taaagagcca gacgccggcg aagatgagcg gcaacatcac
    12121 gctcgctgcc gcgaagcttg ccttcggcac ctcgctcaaa aacgccctca acaatacgga
    12181 tatgatcagc aaggggagcc gcagaagcat aaaacgcagt ggaaagctga gcagcatgcc
    12241 ttcggtaggt aacgcccgga tcggatagga acacaaaatc agcgtactgc tctgcgacct
    12301 gaaccatgga aagccagtcg cctgcaccat tgagctgagg atcccgcttc cagaccttca
    12361 gtagtggctc acgtcgtatc agcgctgcag aaggagggat gtagttgtca tacgtcaaca
    12421 gctcggctac ttcgttacgg cctccaatat agtcctcctc ccgatacccc ggatgccgtg
    12481 gcattgcgag tgcctgtcct tgctcattaa cccacctgat cgatgtatag ccgacaacaa
    12541 ctttagggtt ttccaacaaa agcggtagca ggcgagacag atatccttcg ttgtaaaagt
    12601 catcagccat caacattgac agatagcgac cagtggcaat ctgtataccg ttcaggacgt
    12661 tgtaactcgg ccccaggttc ctctgattgc gtaagtagcg aacgcgcggc tccgcagcaa
    12721 aagcacgcat gacctcttca gtgccatccg tggaggcatt atcgaggact agaacctcaa
    12781 tatcttcata gccttgctcc agcacagagc gtagcgcttg cccaagaaaa cgaccgtggt
    12841 tataggtcgg aatgatgata ctaagaagtg gggcagcttc tctaaccgct gtggcatcgc
    12901 ctgcaatagc tgccgtcagt gcagtaaccc aacagtcact tgaatacgga taactactct
    12961 taccaacaag aaaatcatta agagcaccag cgatttttct accgttttct ttccaatgac
    13021 gatccaactt ttcttggtca gcaaaattgt gaccatccac atagatatcg gccggcaccc
    13081 agtcaggcac ctgccaactc cccatatgga gaggtatatg gcctgcttct aggcactcac
    13141 gccatttcgt tgacaaccac cctggaaagt cgggctggtc atcacatact agaaggcgag
    13201 gttcattaag cggctgatct tccggataca cccccgagac gagcaagggc tgattcaagc
    13261 agactaaatg accattagtg aaaggctcac ccgcaggcgc aaagatccgt gcaaatgttt
    13321 cttcgatctc tgctgaaaaa cgcgcttgtt cccctcgtag taagaggtat cgacgaacct
    13381 gtggattcat cgccaggagg ggctgagcct catgggggca agcaacgagc agaaggccgt
    13441 catagcaatc gctatcagtc cggctgacag gctcaaaaat cagtccattg gagtcgccat
    13501 aagccttcaa ttgctcccaa ggcagctgca gaacaaggtt ttgccaatct gcttcactga
    13561 gtacgaatac ctgttcagga tcaccgcaag ccaagccagc aatccccaca cgccaaggct
    13621 cgccgagaca acgtccaccg gtcatcacat cccactccat tacgtacccc tattattact
    13681 tcgccccgtg gcaagcctag taaattcgaa aacctgctcg ggatggtcaa atacaagatg
    13741 catttaggca ctaggcagag agtgcctgac tgcctatttt cccttgtatc cgttagccgg
    13801 acccgaatta agaaagcgca tgaaaggtaa gaagatcatc gaacaagcaa ccatgttcta
    13861 cgcccttctc cagcaaatgc ctctccgcat aatcacgtcc agcgaaggtg caaatgaata
    13921 cgtatatgcc ctcgcgtcgc aactcatcaa gcacgcctga gcggactacg ggaacgccgc
    13981 aaaaactatc cgactgcaaa tactcatcga ccacagtgac cagctcactt cctggccaca
    14041 gttcccgtag cgcacgggtc aggcggacac catgggtatt cgccccccag aatgcaaaac
    14101 gcagaggctt ctctttatct agcaacagac gttgtagagt ttgcaccatt agattgttgt
    14161 agacacttcg ctcacgggac tcgtaaaaac cgctgatttc agccagtggc gcccagcgtg
    14221 cccgtgcggc ctcgatctgt ttggcggccg cactacggat catctgcttg ctatcttcga
    14281 tatccggcac ccccacctgc cagtcaatct cgccgaaggt ttctcgggtg tggatcttca
    14341 cgtaacgatc cagccaggcg aagcgatcac tgatgttgtg cgccaccacg atggtaggga
    14401 tacccatggc tatacagggt gcggcgcagt gtaagcggct ggtcaccacc aactggcctt
    14461 catcggcata gcgccggcaa agctctcggg ccagggcgtc aaggcggtcg gcctcatcct
    14521 gatcagaggg aatccgctcg tagggataaa tatgagtaag ccgctccacc ctgtctgcca
    14581 actccgcggg cacgaactca agcagttcat cgggcacgtc taccagaaac accttgccat
    14641 ccaccggcgc gtactcgcgg cgagggaaag tgatggtcag gcagcccgaa acaaaggctg
    14701 atatgccacg ttcccggaac atgtgcatga cgcgctcgtc gcgggtccca ataggctcat
    14761 ggcgacggaa atgctccagg tccgcgggat catctacgtc gctggaaaga ccgaaactga
    14821 agtacacggg gatgatgtag ggggacatgg gcagcgggct ttgtgcatta cccaaggtga
    14881 aataaccgca catcggcatc aggatgtagc cgtagcgccc atcgtagtta cggaactcgt
    14941 aacggttcag ccgcacgatt tgctcgggtg caatccccat gcaggcatac aggtgttcga
    15001 ccgcatgggt ctgaatatcg tcgccgagat tgctccggct tccggccgtc aggtggccga
    15061 aggtcataac attggtaatc aggccgtatt tcatggccgt tggcgccagc atggatcgaa
    15121 cctctatcgg gctgggagtt cccattggga aatcacctca gcgtgcttct gcggatgcat
    15181 gaagatgtag ttggtctgga cggtttcttc ggtaacctga gggcagggct gaagtctgcc
    15241 gccatcgaca aagacccagg cccggtagcc gtactcctct agcagagcaa acacgtcgtt
    15301 ggggtggtaa ccaaacgccc gcgaccactt gcgcagtagc tccatcagga tgatcggcgt
    15361 gctctccaag gtcttcgttc cgccctgcac caccatcaac tccgcgccct cgacatcaat
    15421 tttcaatagc gaagggacga tgttgcgggt agtgcagaaa ctatcgaggg tggtggtccg
    15481 gcacactacg ttctggatcg aagtgcggcc ccgagactgc ccagtcagct tcagcgaagt
    15541 ggcaccggaa acatcagggg cataaaggaa gttcagttcg tcctccttat caaacatgcc
    15601 catacaaatg gtctcgatgt tatccagacg gttgaggcga atatttttct ccagcaccgt
    15661 gacggtggtt ggaatgggct cgaaagcgta aatctgcgaa gtggggcatt tgcgaccgag
    15721 caccagggaa taccaaccga ggttggcacc gatatcgaga aacacctcgc catcgcggaa
    15781 caccgacttg aggaaagcag tctcgtcagt ctcgtaactg ccaaaatcca acagggtgta
    15841 aggcgtggcg tgcagatcca cggaatcgac cagcatcgag atgtcatgag ctgcggagct
    15901 gaccgaaacg ccctcggcgg tgatacgtat ttctgccaca tcggtgccgg caagaaattc
    15961 gggatagtcg aaaagcccag cgtgctggtc gttgatgtat tcgccgtact ggaatttgtc
    16021 gatttcgcca gtcatgaacc ttttcctggc caagacaagg cgattcattg cggtattttc
    16081 catgtagggt tttccgatca agactggata aagtaacgca gggttcttgc gaaccctgct
    16141 tcgggtagga tgcttggccg ccagcccagc ccctccattt tggagagatt gaaatggcca
    16201 ctggaacgga aaagcgcttg ccgttcgggg ttcggcggcg gatgttttac ctgcaagtta
    16261 ggcgtgggag aaagccgtac gatcaacttc gccaattcga ggatagtagt ttcctgatcc
    16321 aggccaacgt tgtaagcatt accaccctct ccccgtagca aaaggatcag aaaggcggcg
    16381 gtggtatcag atacgtagca gaagggccgg ctaccagagc catcgctgtt gagcacgatg
    16441 tcgcgcccag ccagcgcatc cgcaataaag tcagcaaagg aacgtccgtc atccagcgca
    16501 atacccggac cataggtatg ggaaatacgc gccattacag tagccacgcc gtactgacgg
    16561 tggtaatcgg cacacaaggt ctcggccatt cgtttgcttt caccataaca agcggacaca
    16621 tccagcgggt caagcacgcc aaagtcatac tcaccgatgg ggctgtcact ctcaccatgg
    16681 ccgtaaaccg cgccactgct cataaaaagc agtcgtgctc catgcaactt ggctagttcc
    16741 agcaaattga gcatgcctac tacattagcg cgtgtggtgc cgacgggatc actcaggtat
    16801 tgcttggggc tgcctgcgcc ggccgcatgc acgatgaaat ccacccgttc cagagtggga
    16861 acagggtcgc agacattctg gatgatgggt cggaaccagc tctgcccgac caggtgaggg
    16921 aagcgccgcc gcagtttttc cgggtctcgc gccaaagcat ggatgatcac aggtcgctcc
    16981 ggccagcgct cgctcagtga ggcgaagaac tccaccagat aagccgcaag gaagcttgtg
    17041 gcgccagcga tgagtacatg actgccggca aagcgctgcc agtcctcagg tcgagaaaag
    17101 atgagctgca agtcctcagc gatcaccggg tggcggcacg gaacacccat cggtggattc
    17161 atctcagtcc agcctcgcca gtacacccat cacttcacca cgcgaccagc ccacatccac
    17221 aggtaggcgc aggacatcaa ccaccgtcag gcttaggcta cctagcaggc gacgcaccgc
    17281 ggtttcggtg taaaaattga tgtgttcgtg ccagtgccgc ttgagcgctg ccagttccaa
    17341 gctatctgga tgctcacgta ccagagcctc atggggcacc tcaaggtaca gcagcgtgcc
    17401 cgatttcaaa gctggcagca ttcgctgcac caactccaga gggaatggaa cgtgctccaa
    17461 cacttggcta caggtcacca gatcgtattg gctctggccg atcagcccga ggtcgacacg
    17521 ctcggcgcca ggcaccagct caacgccaga aatatcatga atgtgcaaca ggcgattaca
    17581 ctcacggaac aggctgttaa taccagtatc gcccccccag tccagcactg ccggtgtatc
    17641 tggaagatgg ggtgccaacc aggcctccac ctcactcaga taggcggcac gctgatggta
    17701 gtgcggcacc acgttgacat agcccggctc gtaacgaatg cgctgggcgt tgtaatgctc
    17761 atcgcggtag ttgtggtaaa gcgctgccat ctgctcgtcg gtaaagcgaa aatcgaggaa
    17821 cagcgcacca cactcttgac attgcaatga gttgcacagg gtgtacgcca tgccctggcg
    17881 caagtcgcgc agaccccatt cggcggtaat ctccacgggc tcatggccga acactcgctg
    17941 ggcgacgaag ggcatcagca ctgcaggaga gcggttcagc tgcgtactgc cacagcagat
    18001 gcagtgccgg gcaatcagtt cgcgactcac catatgctac ggcctccatc gaccaccaaa
    18061 ttggcgccgt tcatataaga cgaggcgtcc gaacagagaa aggcgatagc tcctcggtac
    18121 tcatcggcct cggccatgcg gccaagggga atcagttggc tcagttggct gacaaataca
    18181 tcattctgcc cggcataaac cccgccggga gacagggcgt tacagcgtac gccctgctca
    18241 caccagtagg tggccaggta cttggtcagg ccgatcagtc cgtgcttgac cactgagtag
    18301 gtcactggct tgaccggttg ctggtcgggc gcgagcccct cgactcggta aagacgctgg
    18361 tcaggtgcga tcacccccag atcagaggca atattgacga tcaccccggt gccttgccgg
    18421 gccatctgcg caccgaaaac cttggcacag ttgaatgcac cgccaagccc gacatttaaa
    18481 tccagctgcc actgttccca cggaaaatgc tcaagacgcg agaagtcctt gtcgccggcg
    18541 ctctccacct tgggattgcg tgcggcgttg ttgatcagga tatccacccg tccatggcgc
    18601 gccatcagtt gctcgaaggc cctgagaacg gcgtccagat ccgtgatatc agccaccagt
    18661 gcctgagcct cacaaccgct ttcttcggtc agccgagcca cattactggc aagacctgcg
    18721 gtattgatgt cgagcagtac cggcacgcca ccgagaccgg caatggtcgc ggcatgctgg
    18781 taaccgagca aaccggcgcc accggtaacg accgcgaccc gaccatccag gcggaacagg
    18841 ctttccatgc tagccatgga tattcacctc attcaatgcc acctctctgc cctcattgag
    18901 tgagcgcagc gcggctaaag caaggcgcaa gctctgcaca ccgtcgcgaa gactcaccag
    18961 cggcgcagca ccactgccca agcaatcaag gaaatgccgg gcctgagcaa ggtacatgtc
    19021 attacgtacg aaaccaggga aatcgtcctg ctcgcacagc gcgccttgag catcgaaaac
    19081 ccgcagacga ttggccagta aatcgatctc ggcaacgcca caatcgccaa caactttgaa
    19141 ggtacggact gctggacgtt gcaggaaatc ctggtgaagc agaataggaa agctgccggc
    19201 ctcgccgctg caacgcatca gtgaagacgc ggtgtcctcc acgtcgatct ccaaactact
    19261 caattgcccc ccatgggtga caatgctgag cggtaggcca aatagccagc agatcagatc
    19321 catctcgtga atctgcgaac ggattacccc accaccctgc acagcacgcg atgcgtacat
    19381 ctgccgataa tcctcataac gatgccagtc aggtaggtgc tcgccgatct caccgcgagc
    19441 ggtaagcaga cgaccgaaga acccctcgtc taccagtgtc ttgatgcgtt gcaggccggg
    19501 gtggaaacgg tagttgtagc ccacatggaa caacaggttc ttttcacgca ccaaggcatc
    19561 cagttcagcg ataccttcca gctcactggc cagcggtttt tccatgaaaa tagccacgcc
    19621 tgcatgggcg caggccaagg ccaccgggat gtgtagcgaa ttgggattgc agataaaggc
    19681 ggcatccggg ttactcgcta aggcctgcgc caaactctcg tgaacgacaa caccatagtc
    19741 acgttcaagg tcggcgcctt cttcgacgcc cccctccttc agcttgatac gttgcccccg
    19801 cacgcgatag gcgtgtacct gtaactcatc acccaacaat tgtcgcaggt tgcgcagatg
    19861 gcgctggcca atgctaccca ggccgcagaa cagaaccttc acaccctcac ctcatctgtc
    19921 atcatcccgc cacttagcca gttgaccgtt ctcgccgcca tacgcgcctg ccaagcaggc
    19981 tcttgaccgg attcccccct ggcagcttcc atgacgaaac taccctgata gtcgatctgt
    20041 cgtagcaact cgcgcagcaa cggaaaatcc gtgtccccct cccctaacgg caccgtgccg
    20101 gcgtttcgcc gacgatcctt aacatgcacg ctgttgatcc aggagccata ggctttgaat
    20161 tcatctcgcg gatcaaaacc ggctgctgcg ctattgccgg tgtcgtagtt cacgccgacc
    20221 atgggatggt caatctccaa gagcagctca cgaaaaccct ggggcggcaa gtcactctca
    20281 agattgagac gaacaccact ctcttgcgcc agaggtaaca cctggcgcag gcaagcgacg
    20341 acgatctcgc gctcctgggg tgtcttgatg ctcgacgtat ccaacaatgg caagacgatg
    20401 tgccgaatac ccacctcggg gcagatccca atcagccaaa tcaaccgctc gcaacgctcg
    20461 agacgctgtg cttccgagca gcgcactagc gggcagtcca tgaaatagtc agcgcacact
    20521 gacacaagct ctataccact tcgcactaga cagtcattca tatgccgccg gcctgcagcg
    20581 gtttccaatg gattggcgcc ctcgccgtac aggtcgtata tccactcaag cccacgaatc
    20641 gaaggcgtag agccgagtgt ttgcagctca gcctcccagg tagtgcgggg gaacgactgg
    20701 tagtgcccat ccagctgggg gccaagacgc ccctgcttgg tataaacccc cgcagcagca
    20761 taagtagcca gataatctgc aggcatcttc actcctgaac cagcggtacc agcatattgg
    20821 cttccagctc cccacgatcc agaaaagggt acaaatcctc cagcgggctg gacaccatta
    20881 ccccgttgtc accgatacgg gaggtcaccc gtgggccaat tggctgatct ggctcgacca
    20941 gcacctcgca tacaaccggc cctgggtaat ccaacacctc gcggatcgtc gccgataagg
    21001 cggcctgatc actcacctgc acgctgcgaa caccaaatgc acccgccagc gccacaatgt
    21061 tcggcaaact catgccggat gccgggtcac aaccgatggt ttccttgaaa taacccgtct
    21121 gcgaggcgcg aatagaggca taaccattgt tgttcagtac gaaatacttg atcggcaatt
    21181 gcagccgacg caccacttcc agctcctgga tattcatgac gaaaccacca tcaccatcaa
    21241 ctgagatggt gcgtttgctg ccagccccca aacagccccc caatgctgcc gggagaccgt
    21301 aacccatgga gccgagccca cccgtggcga cagtgcgctg cccgcgcttg agtttggccg
    21361 aaatccagaa ggtatctatg gcagcacccg agctaccggg aatgatcagt tcgccttcat
    21421 ccagtgcgtc gcacagggtt tcggtgaaca catacgtatt cacgaacccg cgctgttcac
    21481 ggtattcagg taataccact ggatagcgcg ttttccactc cgtacagcgc gccagccaat
    21541 catctaactg caggcgctcg gtaggcatgc aactgctcaa accatcaata aagacacccg
    21601 catcgcaaac aaagctctcg tcgggcatat cgcccagctt ggcgatttct gccggatcga
    21661 tatccaccac gataacctcc gcctgacggg cgaagtggct gcgattgaaa ccggtgatgg
    21721 aaaagtccat gcggcaaccg atggccagca ccagatcagc attctgcacc gtgaagttgg
    21781 caccgcgtgg cgccacagta ccgcagcgtc cgaaatacag agggtgctcg tactccagca
    21841 ggtcggcacc aatccaagtg gtcagggtcg ggatacccaa ctgctcaacc agtttacgca
    21901 tgcccgcttc agccgccgca ccatggacac cgttacccac caggagtacc gggcgctggg
    21961 cctccaccag gcgctgccct aaatgctgca atgcctggat gtctagtacg ggcggctgat
    22021 ccatgacatc aggggtgtaa ccttgcagtt gctcagggtc gacctctgtc gcctgcacgt
    22081 ccagcggcac atcgagccaa acggggcctt tgcgtccccc tagcatcaga tgaagggcct
    22141 tttccaactc atagcggacc cgtaatgggt cgagcaaaca gaccgcatat ttacttagtg
    22201 gctgcacaac actgacgata tccagctcct gtgtgcctaa ctggcgcaag ccactgctgc
    22261 ctttgaggtc ggcacgcttg acctggccgg agatgaacat caccggcgtg gactcgaacc
    22321 aagcgcctgc cagcccggta atggcattgg tggcgccagg gccggaagta accaggcaag
    22381 caccaaactt gccagaggta cgcgcatagc cttccgccgc gatggccgct ccctgctcgt
    22441 gcagcatgct tacggcagac agacgcggct ggcgcaacag agcgttattt aggtgcatag
    22501 ccccgccacc gggcaggaag aacacatggc caacgccctg gttggcgata aactcgaaaa
    22561 tgtaatcggc aacacgcatg aggttacggc tcacaacagg aattaagagg gacacgccca
    22621 gcgggcacgg catacagcac taaatcccag ccatcactga catgggggca caggctgaaa
    22681 cgatagccat cgtggtagct gtgtaactgc agaaggatgc gccacaggtg gttctgctcg
    22741 tggtaaacac tgaccgcgat caccggttga cactcactga tcaaacggcg ccccccggcc
    22801 aggcactgcg gttcgaaacc ttcgatatca atcttgatga acgtcggtaa catgccctgc
    22861 gcaacgagat catccagcgt agtcatcgtt acctgctcgt cgccaaatcc cacacgcgaa
    22921 gaggggccgt tggctgcctc gatcagtacc tcaccttgac tatcacccac cgccagacga
    22981 tgggcgtaga caccgggctg cgccaggcgc tctagccgtg cctgcagcgc cgtgaagttg
    23041 ctcgctaccg gctcaaaaca gtggatcgca ctgaaactgc ggcccgacgc cagatatccc
    23101 tctaatgtgt cgccattgaa cgccccaaga tcaaacacca cctcaccgct gaagtcggtg
    23161 ctgaaatgag ggtcgaagta gattggtaac ggggaagaac cgggcagcag gtcatgatcc
    23221 aaatgcaatc gccaggcgac atgatcaacg aacaagcggc gcgatgcctc atcgccaagt
    23281 aacgaaaagg catactggat agcatcaacc tcacccaaca ctttctccgg caagtccatc
    23341 gagtagtgcg gaagaaaatg ctcggggtaa cgccaagcca gatggccgaa tagggcgata
    23401 tgctcgaagc ccagctcacg taatggttgc agacggtcgg ccatgcggtc atggatatgc
    23461 ccactccaga tcgtgcagat aaccgcgggt aagtcaccat gccactgttc agccaactgc
    23521 gcagggctgc ataccggtac agagtcgata tgggtccccc acaatgccgg attgttgtcc
    23581 ataaaggcgc agggtgtcag gcccatgcct ttgagtacgg tctgcacctt gcgtccgaga
    23641 tgactggcgc caaacagtgc aaagggccgc tcaaaaccgc caatcaagcg cgtcaaggta
    23701 tccgattggc gttcgcggac gaccgtgagg ggttcattga gcaggctctt gagcctgctc
    23761 tcaagggttt cagtgctcat gattgggtcc ctttgggcag acgactttcc gggatggcat
    23821 acagcacgat gtcccaggcc ttctcataaa gatgctgccc aagcatgaag tggtagcctg
    23881 catggtacga gtgaacctgc aacaagaggc gccaaaggtg atcctgctca tggtaggcgc
    23941 acacggccag tgctggctgc ttctcggcga tcacccgacg ggcaccggcc aggcactgcg
    24001 gctcgaagcc ttcgatgtcc attttaataa agctaggcgg tgttaaatgc tcggccagca
    24061 gatcgagtgt ggtcatggat accatcccat caccctctcc gactttgagc gtatgccccc
    24121 cttccacgcc aatggcaatc gagcctgcat gatcgcctac tgccagaaga tgagcctgca
    24181 caccaagatg cccagactca acaagcccgg agatggtttt ctgcaaatca tcgaagtttc
    24241 cttctgaggg ctcgaaggaa tgaatctccc gataacgacg gccactttcc aaatagccga
    24301 ggatggaatc gccattgaag gcacctatgt cgtacagcac ttcagcgtca tcgctggcac
    24361 tgaaccgatc actgaagtaa atcgcctctc cactttccgc cggcaacaaa tccgagtcga
    24421 gcgtcagacg ccaggcaaca tgcgcaacat acaagttgcg cgagtcctca tcggcgagca
    24481 agtggaacgc ttcacgcaca cggtcagctt ctgcaagcac ctgctcgggt aaatccaggc
    24541 actgataggg cagcaactga ggaaaccgcc acgccagatg cccgaacaac gcaatacgct
    24601 gaaaccccag agaacgcaga ggtgctcgcc acccctccag gtgagaggtg ccgactcgca
    24661 cggcaccaat gctggcaact accgcgggca aacaaccggc catgcgctca gccaattgtg
    24721 caggcccgag cacctcaata ccgtcgatgt tgcttcccca tagcgcgggg ttgttatcga
    24781 tgaaggctaa tggacacaaa cctgcagcac gtagcaccgg tagcacctga cgccccaggc
    24841 caccggcgcc gaacaataca aagggggcct gctcccctcc catgatcagc cccaacgttt
    24901 cgcgctgacg ctgacgcaca gacgccaacg actcgctcag cagactttcg agtcgggact
    24961 cggcatcgcc tctactggtc agattcatct tgcctccagc tcactcaaga aacgcagcgc
    25021 cgcccagaag cctgcaccat gatccttgtg cccctgccaa acctctggaa tgaaactgac
    25081 ttcaggatgc ctgatgagct gcgcccaagt ggccggccag tcgatatcac cggtgcccat
    25141 cagcacgccc tcgccgttgc tgcctttggc atccgcgacg tgcaggtgag cggagtacgg
    25201 caggagcaac gccagcgcag cctggaaatc gaggttgaag tgaaagcagg tcatctgcag
    25261 atgggacaaa tccaggcaca ggcgcaggcc atgctcacgg gcctgtgcgg caagctcgtc
    25321 gggcatcatg aaaatgttct ggtggcgctg gccgccaaaa tgccagggaa acggcgccat
    25381 gttctgcggg atgagttcag taccggcgaa gtcgatacgt gagcaggcgt catggaaacg
    25441 ctcatacagt tgcgcacgca tggccagtgg ccgcggctcg tcggcagaaa agccgccaat
    25501 attggccaca atcaggcggg catccgccct ggggaagaac tcaccgatct gcagggttgc
    25561 atcgaccaca cgctgcaggt tgtcgatcga acgctgccga taagctggat catccgcgac
    25621 caggtcgagc aactcactgt tttcaaacag ctccggcgcg tgtaccacca agcgcgaaca
    25681 ggccacttcg ctgaggaagg gttggggctg caaactgagg tcacgatagg acaggtgaaa
    25741 ctcgtagaga tcaggctcga tacgccggcg gtagtcgctg aaatcgtgat agcgcaccgg
    25801 cacgccccag cgaattggca gagcaaaggc aagcgactgc tgcgcttgct gttcgtcgag
    25861 gtcactgtca aagaggaagt cactctgggc aatatcgcgg gtagccgttt tgccaagcaa
    25921 ctctggtaat cgatatggcg gcaaaccctg gcctgggcta gccacccgca acatggacgc
    25981 gtcaaggacg gtgccacggg caatgttgca ggcggcgatc acgcttttgc cgaggttctc
    26041 gcgattgagc agttccccct gactggcgtg ccggcccgga ccttgccacg gcaaggccag
    26101 ctccagttga cggatgccct ccaccagttg gcggaactct tccggctcca ggctagccaa
    26161 gtggtccggc ccttccatgt cacggtccag ggtgatgtgc cgctcgacca gtttcgcacc
    26221 cagcgcgaca gcgcccaagg tcactgcaat accgcgctcg tgacccgagt aaccgaccag
    26281 atcatgcaac tcctgcaagc gccgcagata gccaagctgg atatcgactt caggagccgg
    26341 gtaggcactg ttgcaatgca gcaaggcgaa gggggtgccg agggtattca gttgttcgat
    26401 ggcacgcaca atctcatgct cgaaggacat accagtggag aggattagcg gtttgcccag
    26461 gctcgccgct ttggcaatca ggtaggggtt gcacaagtcg gccgaggcga ttttcagcgc
    26521 ggcgacatcg aaatccgcca aggcatcgac actcggctcg tcccaaggcg tgcacaggta
    26581 aatgatgccc ttctgcgcgc agtaagcccg tagctggcga tgcgcatcga ggctcagctc
    26641 aactttatcc aggagatcct gaatatattc aacgccgaga tcctcagccc gcgaaccgtc
    26701 tgccttgcga cgatagagcg catcgcggtt gcgcagttgg aatttgacac aatcggcacc
    26761 agccgcgagc gctgcgtcca ctaactgccg agccagttct agcgagccat tgtggttatt
    26821 gcctgcttcg gcaatcacaa agacacggtc gccgcccagc gggacgccgt caataagaat
    26881 agaagtcatt ggattttcgt tcgccatgag gttacgccgc aggattcgaa gcggaagttc
    26941 tgcgtacggc agcccagtgc ccgtgcggtc tgcaccacgc gctgccgatg ttgcggctgg
    27001 acatagaaga gaaagaaacc accgccacca gcacccaata gcttgcctcc caaggcacct
    27061 gcagccagag cggcttcata gatttcgtcg aggcggctgt ggctgatcgc cgaggagaaa
    27121 cgcttcttca gttgccaagc ttcatccagg cacaggccaa aatcgcgtag ctcaccgcgc
    27181 atcaggtaac ggtgcatacg ccggcacaag gcgacactgg cgctgagcag ttcggtctgc
    27241 gaggactctg cggctatctc ctcgcgttgt aactcatgca aacgccccga gtcatgggaa
    27301 atcccggtat cgcaaagcac tagacaggac tccagctcgt tgcgaatcgc ctcttccaat
    27361 cgaatcggat gcaccaggtt gcgctgattc tcgaactcga tcaggttgaa gccgccgaag
    27421 gctgatgcgt actggtcctg ccaaccgccg gcgatgccaa aacacaggcg ctcggcctga
    27481 aaggccagct cggcaatctc ataggtgttc caacggtcct ggcgcaactc gttgaatgcc
    27541 gcgatgctgg caatagtggc cgccgaggac ccacccaacc cagaccccac cggaaagtcc
    27601 gaacgcaaat aaagatcgaa gccatacttg ggtttgatca ccgacacgat ggtcgccaac
    27661 aagctcttct cagcagccgc cagcagagtc ggcagcgaat cgaagtgttc ttcacggtcc
    27721 agatcttcag agaaaatatg gatctgccta tcgtcgcgag gcaccagcgt ggcatgggcg
    27781 taaaggccca cagtgcaact gagcacagcc gcagggtggt cgatgaaaaa gtaagtcagg
    27841 tcagccccgc cgccgcagaa gctcatccgc accggtgccc gcgcgcgcgc caggacgggc
    27901 acttccgcac tggccgccat ctgccgggtg tagacctcga cgagacgccc ctcggcatcc
    27961 aggcccggca gcgcgttatg gcccaggtcg aacgccttca gcaggtcttc ccgcggggtc
    28021 gcaatcgaca cgctacgaaa ctcacggttc atgcaggccg ttacgggctg agcggtcaga
    28081 ccaccctgca acagatagcg gcgtatatca ccgtttgtca cgaccccaag caagcgcatg
    28141 tcctcggcaa cgacgaaggc aatctgcaag ttgcccgact cgatgcactt gatggcatca
    28201 agaatcgtct gttgcatgtc gatagtgaga gccttgacca gcgagctcat cagacagcac
    28261 tcccgagcaa atcagacttg aacgcattca gggagtcggg ggtacctata tccacgaacg
    28321 ctccgcggta ctcacagata tacagccgat gctgacggat aagcgacggc agcaaatcat
    28381 gctccataga acagctagtg atcggcatac ccgccagagc atcagcgcta aaaccataga
    28441 cgcctgcact cacccaaccg ctccccacct gacccttttc ctcgaacccg aggatttcgc
    28501 cctgcacaga gcaacacagg cttccatagc gcgagcgatc ctcaacctgc accccgacca
    28561 gcacctcatt ctcggctgcc gaggctaggc gataggcgga tgagtcgagg aaggtatccg
    28621 cgttaattac cagatagcgc ccgccaggcg gcacttgaag caaggcattc agcacggcac
    28681 caccggttcc cagaggtgtt tcctcgacca ccaactgcag cgccaagccg gactcagcct
    28741 ctagcgaggg cagcaatgcg gccaactgat cggcgcgata atgcgcacag agcaccccac
    28801 gactgactcc ggcctcacgc aactgcctca acaccagcgc taggaatggg cggccatgaa
    28861 tatccaccac cgccttctgc gtatccttga tcaccgtgcg caggcgggtg ccaaagccac
    28921 cacagaggat ataggcgtgc atcaacccac cctcaaaccg aaaaaggttt ccaggcaacg
    28981 agctgcatgc tctaggtgct cctctgcaag accgggccat acgccaatcc agaatgtatc
    29041 gttcatcacc ttgtcggtta tcggcagttg ctcggaaact ctgaagttgc gcccctgcat
    29101 gtagggctgg cgcaccagat tgccggcaaa caacaaacga gtaccgaccc cctgcgcatc
    29161 caggtagcgc agcaattcga cgcgtcggat gccgcaacca tcgcgcaagg tcattggata
    29221 accgaaccaa gacggatcgc tgccaggagt cgcctgcggc aggatcagga aatcgcggca
    29281 gccggccaag cgttcactca gccaggcaaa attgcgctta cgagccagga tgaagtcgtc
    29341 cagccgatcc atctgcgcca atgcgcaggc cgcctgcata tcagtgattt tgaggttgta
    29401 gcccaagtgc gagtatgtgt acttgtggtc gtagccttcc gggaggtcac ccagttgcca
    29461 gcaaaagcgt ttgccacagg tgttgtcctc gccgggcgcg caatagcagt cgcgccccca
    29521 atcacggaat gactcgataa tgcgtttgag cgccggattg ttggtaaaca cggccccgcc
    29581 ctcgcccatg gtgatgtgat gagccgggta aaagctgagc gtgccaatat caccgaatgt
    29641 accaaccatg cgcccctggt agatcgagcc cagggcatca cagcaatctt cgatcagcca
    29701 cagcccgtac ttgtcgcaca aggcacgaac cacactcagg tcgaaagggt tgcccagagt
    29761 atgggccagc atgatggcct tggttttagg gctgatcgct gcctcgatct tcgctgggtc
    29821 gatgttgtac gtaggtaact caacatccac aaaaaccggc acagcgccga attgcaggat
    29881 cgggttgacg gtggtgggaa agcccgcagc cacaccgatc acctcgtccc ccggtttgat
    29941 cgcccgctca ccgagtcgcg gtgaggtcaa cgcggagaat gccagcaggt tggccgatga
    30001 gcctgagttg gtggtaagca cacaacggcg ccccaggtat ttcgccaggc gtttctcaaa
    30061 cgccgcgttg aaacgcccag tggtcagcca gccatccagc acagcctcgg tcattgactg
    30121 caactcgccc gccccgatca ccttacctga aggtggaacc aggctttgcc ccgccacgaa
    30181 cggcttaggg gccatctgca actcggcgta gcgcccgacc aactcagcaa tctgttggcg
    30241 caactgctgc gcagtactgt cactcattga atttcgcctt ggtgaaccgc gatctgctcg
    30301 cgggtaacga cggtcatatc tcgtccttcc tgccaggcca actgccagtc cagggtatgt
    30361 ttcaaggtag cttcaagcga ccatcgcggc acccagccca gagactgccg tgctctactg
    30421 gaatccagcg ccagcaaacc tgcctcatgc ggttgggcgt cggcagatac cgaccagcgc
    30481 gcctcacccg gccaatgttc cgcgaggcgc cccaccacgg cctcaactgt cgccagaccc
    30541 gcgctgtcag gcccgaagtt ccaggctgca gcatgatgct cagggtcgtt cagcaacccc
    30601 tgcgccagtt gcaggtagcc catcagagga tcgagtacgt gctgccaagg acgagtcgca
    30661 tgagggtagc gcaaaacaac ttctcgcccc tcctgccaag cgcggaagat atccggcagc
    30721 aggcgttccg gcgaccaatc accgccgcca agcacattgc cagcacgcgc tgtcgccagc
    30781 gcaacaccgg aggcacggag aaacgagtcg cggtagctgg cacataacaa ttcgacgcac
    30841 gccttgctac tgctataggg atcaaaaccg ccaagggcgt cctgctcacg ataaggccag
    30901 gaccactcgc ggttctcgta gcacttgtcg gtggtcacca ccagtactgc acgcactgaa
    30961 gccgtagcgc ggacggcttc gagaagatta agcgtgccga tgacattggt ggcgtaggtc
    31021 tcggcgggcg cacggtagga ctcgcgcacc aatggctgtg cggccagatg aagaaccaac
    31081 tcaggctgaa acgcctgcat ggctaccgcc agcctgtcag cgtcgcgaat atcggcaaga
    31141 tcaccggcaa cttgcgactc caggtgcgcc acctgccata aggaaggcgt agtcgcagcc
    31201 ggcaaagcga acccacgcaa gtcaacccct aactggcgca accagatcgc caaccacccc
    31261 cccttaaagc cggtatggcc agtgagcagg accttgcggc ctttccagaa agcggggctc
    31321 agcgccataa ctgccactcc gcctccccac ttctccagtg ctcctccagc agtacccggt
    31381 cacgcaaggt atccataggt tgccagaaac cacgatgcag atgactcatc aattgtccct
    31441 cactggccag ggtgcgcatg ggctcgaact cccaggtcgt ggcgtcccca tcgatgtagt
    31501 cgatcactgc cggttccaga acaaaaaaac cgccattaat ccagccccca tcgcccagtg
    31561 gtttctcttg aaagccactg accatttgcc cctgaacatc aagcgcgccg tagcggccgg
    31621 gcggttgcac agctgtcact gtcgccagct tgccgtgctg tttgtggaaa gcgatgagcg
    31681 ctggtatgtc gatattggaa acgccatcac cataggtaaa gcagaaggtc tcatccccca
    31741 aatactcccg tacgcgcttg agacgcccgc cagtgctggt ggcttcgccc gtatcaacga
    31801 gggttacccg ccaaggctca gcatgacgct ggtggatgtg catacgattt tcagccatat
    31861 cgaaggtgac atccgacatg tgcaaaaaat agttggcaaa gtattccttg atgacatagc
    31921 ccttgtagcc cagacaaatc acgaaatcat taatgccgta gtgagaatag attttcatga
    31981 tgtgccagat gatcggctta ccgccgatct cgatcatcgg cttgggcttc agatgagact
    32041 cttcgctgat acgtgtaccc aacccaccag ccagaatgac tactttcata gacgcgccta
    32101 agcctcaaac caaatcgaca atttattggc attattatca gaacagcaaa agacatgccg
    32161 ccctaagaga gtggcacgta tttggaggac ggggaaaatt tgaaccacat tggcaacggc
    32221 ttggagcagc tccaaccgca ataggatcgt aacagcctac aagatgaaga atctagggca
    32281 gcgagcgaca ccaggcgaca gcactttgca gtgcttgaga tatgatctta gagggcagaa
    32341 ggggcaccca tcagtaacgc cgctccaaaa cactaaagag cacagcgcca tagagcctgt
    32401 agccaatcac agcttattaa acaaattcaa actggcaatt ttcacataac tctgctgcga
    32461 cgcttccaga atgatcgtct gaaacgacag tcgcgacaag gcttcaggat agtccaactc
    32521 acgcaggtcg gcctggatgc ccttgttcac cagggcaacg tcctcattgt cggtgagcgt
    32581 ggtatcgata acgttaagcc gcgcaccgat agcgccgcgt acgctgtcga cattgatcat
    32641 gccgttgtcc agattggtga ggcctatggc aacgctgtca cgaatctgcg catccgtgct
    32701 ggcggggtct tccagggtac ggcgcagagt ggcaatggta tcgaggatgc cttgcttctg
    32761 acgcgtagga tcgaccgtga cctgcaagct ttctccaccc aatgcggtgc cgtcaaacag
    32821 aacactaacc cctcgaaaaa ccatctgatc acgagtatcc ggatcatcgt caagggtacc
    32881 actggccagg accggtgccg gggctgcggc accgatctcg aacacctcat agctgttggc
    32941 atctgggtta ccgaagatga cctccacccc tgcctcaggg aaaggcggcg tactgccgaa
    33001 ggccacttca tcttgcacca agggcgccga ggcacgaagc gtggagccag gcgctccagt
    33061 gagatccagg gcaccatcta gacgcccggc attaacgatg ttttcaaaaa tcgctttgcc
    33121 actgtcgctg atcggtactt gcagggagct ggcgatctgc agcttgcgct ggccttcatc
    33181 gccctggtaa ctgtaggagc cgtctccatc ccgcacaaag gcctgggttt ttccctgaaa
    33241 cccggagaat agatattcgc ctcgcgcgtt gcgcgtattg accagcgaga acagctcctc
    33301 ttcacgctca cccagctcag cggcgatgga ttctcggtct tccttactca gcgccccatt
    33361 gcccgcctgt accgtcagct cgcgcacgcg ctgcaggacg gtgttgaccg agttgagcgt
    33421 gacttcctcc tgcgtcaggc tgctgttggc cgcggccagg ttctcgttgt actgccccag
    33481 cacgctctgc tgctgctcga gttgcaacag acgcacggag gcaaccggat catccgcagg
    33541 tgtaaggatg cgattgccgg tactgatttg ctcttgcgta cgcgtcacgt cggcatagtt
    33601 gcgctgcagg ccggtgacgc cattgttgaa tgcctggatc gtggagatgc gcatgaaacc
    33661 ttacctcatg gatgctggag caatacccac cgcagttagc tgaccgccag tgaaacgacg
    33721 tctttacaca ctaccgttac cggaaggcac caatcagggt atcgaacaac gagcgcgcaa
    33781 cttgaatgac ttgcgctgac gcgttgtagt actgctcgaa tttgatcagg ttggccgcct
    33841 cttcatcgag gctcaccccc gacaatgaat ctcggttatc ggtggcctgc ttgagtatcg
    33901 aggtagttgc caggctgtcc tgtcgcgcct gggcagtcaa ggtgccgaca cgctcgaccg
    33961 tgtcgccata gctgtcgacg aaactgctgc ccgtgctgat accggtaatt gtcggatcaa
    34021 ggccaacggt ggctttactc tgcaagtctg ccagggccaa agcattgcgg ttatccgaaa
    34081 caccattctg gttgaacgac aggctaaagg tgtcgccgtt ttccggacga ccactcaggg
    34141 tcatacgcat ccggctgccg tcactcagct ccagttcata ggtgttctgc tgcccggact
    34201 ggaaggtagc cggtgtcgcc ggcgtaccat cattggggtt gatgcgcgtc agcgtagccc
    34261 cagcaggcac ggtaaaatca ctggtgcccg catcgtacgt cagggtaacg ggtggcagca
    34321 gcgccgcctg cagcgcggcg ggatcaatat tgctgccggt gctttgcaac gtcggctggc
    34381 cgatggtcgc ggtaccgcgg ttctgcacgg tggcgtcggc ccgcacgggc gcagcgaaag
    34441 ccagttggtc gggctggtcg agtacggctt cgatggccgt cgcaccgcgg cgtgtcggtt
    34501 gcaacaggta gcgatcattg gccgccgggg cagcatccag ggcaatctcg aagccctgat
    34561 cgcgaccgct ggcatcggtg aagctcagcg cagctggata gctgcccggt gggttggggt
    34621 tgacggtcag ggtcgcgcca tcgctcaggc gccgggccga gtattgcgtg ccatcgaact
    34681 caagacggta gtcactcgtg gtcagcacac tgctgtcggt gatgttcaaa gcgggctgca
    34741 cgttgctggt gttgccggca gaagcacgca ctcgcaactg ggccgtggcc gggtcattga
    34801 aatcaccgaa cagggcggcg cccacttggc ctttgaggtc caagccctgc cccagctgac
    34861 tattcacctg gtccgcgacg gccaatgcca ggcggccgat ggaattgagc gtagggtcga
    34921 gggtttcact gcggtaacgg atcagcccac ccagctcgcc gccagtcagt agcgaggtga
    34981 ccccctgacg cgagccacca ctgacgaact cgatctcgaa acgggtcggg tcatctcgcc
    35041 ccgcgactgc ctccaggcgg ctggcggtgc tgcctactac cagtggctgg ccggagccga
    35101 caaacacgtt cagcgaactg tcgtcctgcg ccaccacact gacaccgata taggtagaca
    35161 gttggcgtac cgcttcttca cgggcatcga gcaggtcgtt gggttgcttg ccattggccg
    35221 ctgcgacggc aatggcctcg tttaaactgg ctatcgaggt ggccagacga ttgacctgtt
    35281 cggtcacggc cgacatctgc ttattgatga actcgttctg ctcgctgacg cggtcgtaga
    35341 ccgtattgaa acgcttggcc agaccttcgg cctcagacag caccaactgc cgagcaggga
    35401 tattcgccgg gtcttcggcc gctgtctgca aggctgagaa gaacgactgc agcgacggtg
    35461 taataccagt ggtggtacct gccaacaaag agtcgagttg ctcgatctgc gccttgtaag
    35521 cctccacatc ggcgctcagc gccgtgctgc tgcgcacttg cgcggtcaaa aactcgctgt
    35581 aggtgcgacg gatatcaacc agggtcgtgc ccgagccgat atagccaaca ccactgaact
    35641 gcggcacacg ggtggcctgt acagcatcct gacgtgaata gccgggcgta ttgacgttgg
    35701 tgatgttgtg ccccgtgacc gccagcgagg tcttgttcgc cgacagccca gacaggccaa
    35761 tattgagtaa gtcagccatc tttcatcatc cgttggtgga cggcgtactg acggccgcga
    35821 ctgtttgata ggtctgcatc tgccgtgcta tctgggcaat tttgcgagca tattgcgggt
    35881 ctgtggcata tccggctttc tgcaattcac gggcgaactg ctcaggcttg tcggtgctgg
    35941 ccagggcttt ctcgtaacgg ccattgccct gcaggaagct cacaaaatcg ttaaagctgt
    36001 gctcgaagga gtcataggcg cggaacgaag ccgcttcctt gaccgccttg ccgcccttga
    36061 actcggtggt cagtactcgc gccgagtcgc cttcccaggt gttgtgagtc ttgataccga
    36121 acaggttgtg gctgctggtg ccgtcctgct ggcgaatgat cgacttgccc caaccggttt
    36181 ccagcgcagc ctgggccacc aagtaacgcg gatcgacgcc aatcttctcg gcagcctgct
    36241 cggccatcgg cagcatggcg gcaataaatt catccttcga accgaacgcg gatttgcccg
    36301 gtgccagtgg cggctgagca atacgccgcc cggtgatcgc atccatacca ttcaagccca
    36361 gcgcagtatc agcaggtgcg gcaaaagact tggctggaat ccagtcattc tgtgccaatg
    36421 gctgaccatc ggcggcagtg gccgatggca caatgcccgc tagcagacgg tccgtcagtt
    36481 tgcccggtag agacaggcgc cgctggttga tcaatgaagt gtcgtcacgc gcactgtcga
    36541 ccttggccag tggcttgctc ggcgcactcg gcaccggctc attgacctga gcaaacggat
    36601 taggtcggct atccgcctgt ttcatcttcg acatttgccg caccagcacg tcagccaggc
    36661 caatgccgtt ctgatgattg gacagagtca ccgacagctg ctggtcgtgc atgtcctgat
    36721 aggttttgct ctcgttgctg ttcatgaagt tgccttcacc gaacgcttcg ttggccgagc
    36781 gcatggcctt gagcatttca ttaagaaaca gcgactcgaa ttcctgagcc acctttttga
    36841 tgttcttttc gctgtcgcca ccaaccttga actggttgag gcgattaaga tcgttatagg
    36901 caccactgtc gacggtgctg gtgccgccga gaaaaccggc tgaaaggcga gagtccatag
    36961 cgtgcgctcc cttaaatcac gatcagatca gcctgcagag cgcctgcttg cttcagcgcc
    37021 tccaggatgg ccataaggtc ggaaggcgaa gcgcccacct gattcaccgc acggacgatt
    37081 tcatccagcg tggtcccggg accgaacttg aacatcggct tggcttcttc atcggcattc
    37141 acccgcgagc gcggcaccac ggcagtctgg ccgccagaca gtgcttccgg ctggctgaca
    37201 atcggatctt cggtgatggt tacggtgagg ctgccgtggg tcactgcggc aggctgcacg
    37261 cgcacgttct ggccaatcac gatggtgccg gtgcgcgagt tgatgatgac cttggccaca
    37321 gcctggccga tctcgacttc gaggttttcc agaatcgaca ggtagtccac ccgctgaccc
    37381 ggatccagcg gggcactgac acgaatcgag ccaccatcca ccgcctgcgc gacgccaggg
    37441 ccaagcagtt cgttgatatg gtcgacgata ttcttcgcgg tggtgaaatc cggacgattg
    37501 aggttcagcg tcagggtgtt gccctgatca aagccactgg gcactggccg ctcgaccgtc
    37561 gcgccacccg gaatgcgtcc ggccgacggt acgttgacgg taatccgcga gccgtcgccg
    37621 ccttcggcgt caaaaccacc gaccaccaga ttgccctggg caatcgcata gacgttgccg
    37681 tcgatcccct tgagcggcgt catcagcaag ctaccgccac gcaggctctt ggcgttaccg
    37741 atggaggaca cggtgatgtc gatggtctgc ccaggcttgg cgaatgctgg caggtcggca
    37801 tggatcgaca ccgcggcaac gttcttcagc tgcacgttgc cgccctgcgg caccttgatg
    37861 ccgaactgcg ccatcatgtt gttgaaggtc tgcacggtaa atggcgtttg ggtagtctgg
    37921 tcgccactgc cgttgaggcc gaccaccagg ccgtagccaa tcaactggtt agtccgcacg
    37981 ccctggatgc tggccagatc cttaagccgc tcggcctgag ccagcgaagc cagcaacaga
    38041 gcggcggcag ccagtaggta cttggaaagg gtcatggtgg ccatcctcag aacggccaca
    38101 ttgggctaat aaagaaacga ctcatccagc cgggctggga cgcatcggca aaggcgccag
    38161 tccccgagta ggtgatgcgc gcatcggcaa tccgggtcga ggaaacagtg ttgtcggtag
    38221 cgatgtcatc agcccgaacc aggccagcaa tgcgcaccaa ctcatcacca gtattgaggg
    38281 tcatccactt ttccccgcgc accagcagca cgccgttggg catcacctcg gccactgaca
    38341 cggtaatcga gccggtcagg ctgttgctct gcccggccgc gccggaacca tctgctgaac
    38401 gggtggcatc aaagctggcg tccaggccca gggtgtcgtt ggtcatgccc agcgcgctaa
    38461 ggcctgccag ggggtttttc ggggctacgg tcatgccgaa caggttaggc acacccaggt
    38521 tggccgagct gtctttgctg atctggctat tgctgttttt gctggcctgg gtgcgctcat
    38581 tcagggtgat ggtgatgatg tcacccacgc ggtaggcctt gcgatcgccg aacaggccat
    38641 tttcgaaccc ggcctgatag atcgaaccat tgttctgtgc cgctggcagc ggtgtacgcg
    38701 gcatcaccgg agcgtaatag ggatcatccg gcttaggcgc aggagccatg cagccactca
    38761 aagccagggg gccaagcagt gcacttatga ttatcagccg gttcatatct actacctcat
    38821 gcgttgcctg gggtggcgtg taacgtgagc caccagtgcc agttcaagcg attccttgct
    38881 gttatcaggg ccaagccagc cgcagcgcgg ctaacccacc tacctatcaa agattctgcg
    38941 aaataaagcc gagcatctgg tccgcggtgg aaatcacctt ggagttcatt tcataggcgc
    39001 gctgagtggt gatcatgttc accagttcct cgaccacgct gacgttggag ttttccaggg
    39061 tgttctgcag tgtggtgccc aagccgttca ggccaggagt gccgacctgc ggtgcgccgc
    39121 tggaagcggt ttcaagaaac aggttgttgc cgatcgcttc cagacccgcc gggttgatga
    39181 agtcagcggt ctgcaggttg ccgacgatct gcggctgcgg gttaccggcg gtggtcaccg
    39241 acacggtacc gtcctcgccc acggtaaagg tgcgcacttc attgggcagt acgatggcgg
    39301 gctctagggc atagccgttg gacgtcacca cctgaccatc ggagttcaga tgaaagctac
    39361 cgtcgcggct ataggacacc gtgccgtcag gcagcagcac ctggaaaaag ccacggccgt
    39421 tgatcgccat atccagcggt tgctcggtgg tttgcaggct gccagcagtg aaaatcttct
    39481 gcgtacccac cacccgcaca ccagtaccca gttgcagacc ggaaggcaat tgactgtcct
    39541 ggctggactg accacccggc tgacgacgga tctggtacag caggtcctca aactcggcgc
    39601 gatcacgttt gaagcccgtg gtcgaaacgt tggccaggtt gttggaaatg gtggtcaggt
    39661 tcatgtcctg agcggacaga ccggtcttgc tgacccacag tgccggaagc atatcgattc
    39721 tcctcgggcg ccgttttaac gacgccgcgt gctgataatt aggacatctg caagacgcgc
    39781 gccatggccg acgaattgtc ttcggcggtg cgcatcatct tcacttgcaa ttcgaattgg
    39841 cgggacagcg agagaatcgc ggtcatctct tccacggcgt tgacgttgct cgattcgaga
    39901 aagcccgagg tgatttccat attggcgtcc gcctgcacag ggccctggcc cttgaagcgg
    39961 atcatcccgt cggtgccctt ctccatctgc ttgaggtccg ggttgaccaa cttcaagcgg
    40021 tccacctcgg ccagcacgtt gggcgcctcg cccagagcgc ggatgctgat ggtgccgtcc
    40081 ttgccgatct cgattttctg ctctggcggt accgcaatcg gtccggcgtt gcccatcacc
    40141 ggcaaaccat tgccggtacg taacacaccc agagaatcga cgttcaggct ggcggtacgc
    40201 acataggctt cgctgccatc gggggcctgc acggccagcc agcccttacc gcccactgcc
    40261 acatccagat cgcggccggt ttcctgcaga gaacccggag tgaagtcagt ccccgggcgt
    40321 tcgctcatgg cgtagacccg cgacggataa ctgtcaccaa agatcggcat ggaacgcgcc
    40381 tgctcgaaat cccggcggaa accattggtg gtgatgtttg ccaggttgtt ggcatgggca
    40441 cgctgggcca gggtgttgtt gtgggcgcca gtcatggaaa cgtacagcat cttgtccatg
    40501 gttattctcc gagtgggctt gccgccccgc tgctctatac aagctataaa gcaatggctg
    40561 tgccagcact cgaaaaacaa acataagcat ttgataaata tgaatttaaa aaataaaaaa
    40621 ggctccaaat ggagcctttt aaatgccagt gcggcgtact tttgccgcct gcgacaggca
    40681 cgcgcctgcc gcaggcaaca cttatcaacg caggttgata atggtctggg tcaccgcgct
    40741 ctcggtttca atggttttgg cgttggcctg gtagttgcgc tgcgccacga tcaggttgac
    40801 cagctgatcc gacagctcaa cgttggagtc ttccagcgcg ccagcttgta acgagcccag
    40861 cgtgccactg ccaggcgggt tgcgcaccgg ctcacccgat tcgaacgact gcacccactg
    40921 ggttttgccg accggtgtca ggccctggac gttggcgaaa ctcgacagaa tcacctgccc
    40981 ttgcaccttg gattgaccgt tggtatagcg cgcaaagatc acaccagtct catcaatctc
    41041 caatccggcc agctcacccg tggtgtagcc atcctggctg acccggttta ccgcaaacgc
    41101 actggaaaat tgggttgaac cgcgcatgtc gaaggtaaaa ccacccgggt tgctctcggc
    41161 tccgttgagc gaccaccccg tcggcgagtt ggtatcaggt gctgccggat tccaggtacc
    41221 gatggtgaac tgcccggccg gcgtgatagc cgccccgagc gttggcggga tcgtggtgat
    41281 atcagtgatc tgcgccagcg aactgatttg cccagacgca tcaaaattta actgatagcg
    41341 atctggctca gtacctaccg gtgccgcagc tgctggatca gacgggctgc gcccgtcgat
    41401 caaaatattc atctgccact ggttcgcatc atccttgacg aaatactgcg tcagaacgtg
    41461 ggcattgcct tgagtgtcat aaatattggt cgaagtcgac gagttataag tggtcggatc
    41521 gcccggatca aatggcgtat tggtcgggct cgcaagggtc gaattgaggt tgaagccctg
    41581 ggaaatgctg gtcgtagccc ggggttcctg actggcagtc tgcacctgca aattgcccac
    41641 cacgccctgc tgcaacacgc cattggcatc ggtcgtgtag ccctgcaggt tgtagccgaa
    41701 gttatcgacg ataaagccct cgcggtccgt gccgaagtag ccggcgcggg tgtagctcag
    41761 ggcgccattg ttgttcacct ggaagaaacc attaccattg atcgccaggt ccagtgcgtt
    41821 ctgcgtgtaa ttgatgttgc cctggttgaa cagctgcgac acatcgccca gcaacacacc
    41881 gctaccctgg gggttgctgc cggtgcccag cacagaagag gcgtacacgt ctgcgaactc
    41941 ggcgcgcgac tgcttgaagc ctgcagtgcc ggcgttggcg atgttgttac ctgtcacgtt
    42001 tagatcattg gtcgctgcac gcaggccgct aagaccgata ttgaacgcca tggatgtctc
    42061 ctttgccggt acgccggcgg ctaagcctgt aagttattga ccgatgacct gcacttgaga
    42121 cagcggcaca ctgccgagcc cagccaggtt gagcatcaac tcgctaccac cgagaaccac
    42181 actgtcaacg ttggcaggca acagggtgta aagccctttg gcctcaccgt cataagtcgc
    42241 ctgcgcctcg aacttataag taccgggcgg catcaggttg ccgccactgt ccttgccatc
    42301 ccagataaag ctgacgttgc cggccttctg ctcacccaga ttgacccgcg aaaccactgc
    42361 cccagcgctg tcgtaaacat tgacgtagac gttgctgcta ctagtcggaa gaaccaggct
    42421 cgccttgaaa ctctcactgg tgtcgaccac tgctttatcg gtcggcacga tgaccttgcg
    42481 cccaaccaaa gaagaagcct gcagggcttg ggaagactga tagccggtca acagcgacga
    42541 catactggtg ttgagttttt caatgccttc aacttggctg aactgagcca gctgggcgat
    42601 gaactcgccg ttaccctgag gctcgagcgg gttctgatta ttcagttgcg cgaccagcag
    42661 gttgaggaac tcgttcttgc ccaactcctt gttctgcgcc gtgctgcctt gctgcatctg
    42721 gtactgatcc agaaccgagc tggtgccgct tactgtactc atggtcatcg tcctcgctgg
    42781 gttactgacc cagggtcaat acgcgctgca tcatctgctt ggcggtgttc atcatttcag
    42841 tgttggtctg aaaggcccga ctggcagaaa tcatgtcggc catttcttcc accacattga
    42901 cgttggggta atacacatag ccgccggcat cggctgctgg atggttcggt tcatagcgcg
    42961 gcattaggct gctctgatcc tcgaccacac ccagcacctg tacgccaacc ccggcctcat
    43021 cctgctcggc aaacagcgaa ccgcgatcac cgttctgcgc tgactgaaac atggtggcga
    43081 acaccgggtg acgcgcacgg taggtctgat cgatgcttga ggagaccgtc tcagcgttgg
    43141 cgatgttgct ggcaatggta ttcaggcgag tgctctgggc gctcataccc gttcccgcga
    43201 tgttgaaaac actggctagt gacatggcat cagactccta ttaatcgccg cgtagggcgc
    43261 tgaccagccc tttgaatttg ctgttgagca gggtaaagct ggcctggaac tgcaccgagt
    43321 tttccgcgta attggattgc tcgatctgca ggtcgacggt gttctggtca atcgaaggat
    43381 ggaatggggt gcgatacgcc aggctggcat ctccgacaat cacgccatca gcagggatat
    43441 gctggctatc agtacgattc aggctgacct ggccgcgctg gctcttggcg ctttgctcag
    43501 caagcacagt ggcgaactgc agatcacgcg ctttgtagtt cggcgtgtcg gcattggcga
    43561 tgttgttggc caacacctca gcgcgctgag cgcgaaagcc cagtgcttgt tcatggatgc
    43621 cgagtgcccg atcgaaactg atgctcatgg tcagaaacct ttgccggttg gtcttgctac
    43681 tttccgtgca agaccataag caatgcttgt gccatatatt tttttactta tttatcaatg
    43741 ccttgatcga caaaatcatc gacagaagcg gcaaagcggc aagcgctttc cgcctatggg
    43801 caagcaagcg gcaagtgggt tttgcctgat tgccgctcac gcggaaaatt ccaacggaag
    43861 ctacgtgacc acgactgcgc cactctccac ccgcagccag atcctgcccc gcaaccgtcc
    43921 gtcaggcaat tcgcaatcaa ccacacacag ttttgacaag cattctcatt aacattagta
    43981 tccttcgcac tagccactgc cctgccacga gcctt
//
LOCUS       DASRUM010000007         8156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_505551, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000007 DASRUM010000000
VERSION     DASRUM010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505551"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="VFF22_00485"
     CDS             <1..149
                     /locus_tag="VFF22_00485"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248803.1"
                     /note="converts protoheme IX and farnesyl diphosphate to
                     heme O; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HZX15333.1"
                     /translation="GGRFLYWAVVLYRDSKPHAAISTFKYSIWYLFALFIALLVDHYL
                     LLNL"
     gene            161..793
                     /locus_tag="VFF22_00490"
     CDS             161..793
                     /locus_tag="VFF22_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008027684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZX15334.1"
                     /translation="MTRTQITVFVLVAIVALVLGLTVNKVLTSKGQGDPTVLLDAGIV
                     LLPQSRSLPELSLTNQDGQTVAVDQLKDQWSLLFFGYTFCPDICPATLAQLRQLQGQL
                     PPETLAKLRIVLVTVDPNRDTPEQLKKYLDYFDAGFIGLTGEQATLQKLANGVSIPYI
                     PADTSKENYTVDHSGNLVIIGPDGKQRGFIRAPINNAKLAAQLPGLLDRL"
     gene            complement(798..1223)
                     /locus_tag="VFF22_00495"
     CDS             complement(798..1223)
                     /locus_tag="VFF22_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZX15335.1"
                     /translation="MALAPFHLAIPVYDLAAARHFYGEVFGCAEGRSSDHWVDFDFFG
                     HQLVIHQAPKMAHQEAAHSNPVDGHDVPVPHFGVVLGWEDWQTLAARLQAKGTQFVIE
                     PYVRFAGQVGEQATLFLLDPCGNALEFKAFKDISQLFAK"
     gene            1334..2227
                     /locus_tag="VFF22_00500"
     CDS             1334..2227
                     /locus_tag="VFF22_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZX15336.1"
                     /translation="MLRELKTFVAVARHGTFAAAGQQVGLTQSAVSAQMRVLEQGLGV
                     RLFDRSGRAAVLNVAGRHALPLAEQMLALYGQMARPASLAEWQGELKVGAIASVQTGL
                     LPEALPAFRQAAPRVELKLVPGVSLQLLSQVDAGELDLALLIKPPFDLPKELLQVSLA
                     REPFVLITPLTVAGDDPLQILAEQPFVRYDRSSFGGRQVSQFLREQRLQVHEALELDE
                     LDAIVRMVECGLGVSLIPRAGLWLQRSSQLRVIDLGALTFHRELVALLRRAQQQPALE
                     CLLQCLQAQNAIGVRSTAPPV"
     gene            2499..4028
                     /locus_tag="VFF22_00505"
     CDS             2499..4028
                     /locus_tag="VFF22_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX15337.1"
                     /translation="MRALLYPLLAMLLAGCSPNQDPNQLSIGGPFEFTSQDPSRDGYL
                     YTRLQVAETLLDVDAQGQLLPGLAEAWSQSEDSRVWRFTLRQGVRFHDGAPLDAAAVV
                     HALQVARSKPGVLATAPVEAFEAEDAHTLRIRLSRPYNPLGAVLAHYTTVILSPASYD
                     AKGLVTRLHGTGPYQVERFDPPHRLGVVRFADYWGTPATIERAVYLTGHRAESRALQV
                     MAGQTDIVYTLDPASLDLLGRRDNVRLHSHTIPRTIQLKLNLADPLLAERDTRLALSL
                     AIDRSGLANGVLRVPGGEAGQLVPPSLGDWHLADLPAPQRDLFRARALLAEQGWQAGA
                     DGILQRNGEAFRLRLMTYADRPELFQLATAIQAQWRELGVDLQVSVVNSSDIPAGHHN
                     GTLQVALIARNYGNIADPLGLLLADYGDGGNGDWGAMAWHNSELPVLLEAQQSERDAG
                     RYHARAQRVAQILAEELPLIPVLYYTQQVAVSARVQGFSFDPYERNYRIAEMSFFPCC
                     N"
     gene            4016..4954
                     /locus_tag="VFF22_00510"
     CDS             4016..4954
                     /locus_tag="VFF22_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX15338.1"
                     /translation="MLQLILRRLLQAAFVTWSVGTLSFVLMRLLPGDMEFRIAAGRYG
                     YDHVDLAAAEAVRAELGLDRPGWEVYLHWLGDLLRFNLGDSLVSGLPVSHEIAHLLGH
                     TLLLAGAAMLIALLIAAPVGVWAALRAGGWFDRLSLLVSALLRAQPVFVIGLGLLLVF
                     ALEWRMLPVAGFDGPRYLILPALTLALAMAAVSSRVVRHSAAEVLRSAYFNFARVKGL
                     SLRQALQRHGARNAALPVVVFLGIQLASVIEGVVMIESLFSWPGVGHGLAHAVFNRDV
                     PMIQGAAMALGLLFVALNLLVDLACYLIDPRQRSAH"
     gene            4954..5751
                     /locus_tag="VFF22_00515"
     CDS             4954..5751
                     /locus_tag="VFF22_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX15339.1"
                     /translation="MLRLFASLSTSQRVGAGLLALLVLFAVLEGLLGHTDPALQNLAQ
                     RFTTPSLAEPLGTDPFGRSMLARMGAALRLSLSLSLVCVLTSALIGVGLGIAAGWRQG
                     WVDRVLSFVLNLVLALPGLVLILLVAGLAPGSLLALYVAISLVLWVEYFRVVRAITRQ
                     AVASQPMQASRMLGFGWRYCLRRHVWPALVGPVATLAAFGAAGSIMALAALGFVAVGL
                     RPPTAELGLMMVELFPYYSDAPWVLAQPVLAVFLLVLGLNLLAGKRA"
     gene            5748..7151
                     /locus_tag="VFF22_00520"
     CDS             5748..7151
                     /locus_tag="VFF22_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZX15340.1"
                     /translation="MTILLRIEGLSVQADGKTLLEPLSLNLAAGECLTILGETGAGKS
                     LLMHAIMGTLPPPLTASGRILLGDSDLASLDRSARERLWGQQLAMLPQEPWHSLDPLM
                     PALQQVAEVHECVHGQVPEQARQTATQALTDVGLEAAAARLPSQLSGGMAQRLAFCAA
                     TAAGAPILLADEPTKGLDASRRDQVATLLRQHAQQGALLTITHDVEVARQLGGTLLVM
                     RAGHLLESGDAQAILDAPQHDYTRALIAADPRHWPTPLVAAVTGAPAHEPVLEARGLC
                     LKRGGRQLFEGLDLRIEPGEILGIAGDSGCGKSSLGDLLLGLLPADAGMVTRRPGVAA
                     HRYLKLYQDPPAAFAAGVPLRRLFDDVLRLHRLDPAKLPPLLDRLSLRPELLARPATE
                     VSGGELQRLAIARALLLDPLLLFADEPVSRLDPITAREVTELLVQSARERGCALLLVS
                     HDAEALERACDRHIRLG"
     gene            complement(7361..8131)
                     /locus_tag="VFF22_00525"
     CDS             complement(7361..8131)
                     /locus_tag="VFF22_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HZX15341.1"
                     /translation="MKKLIAAFAALAAFSAQAESLSVAATAVPHAEILEFVKPALAKE
                     GVELNIKVFTDYVQPNVQVAEQRLDANFFQHQPYLDEFNKSRGTELVSVAGVHVEPFG
                     AYSSQHKTLADLPQGANVVIPNDATNGGRALLLLQKAGLITLKPEAGILATPKDIVEN
                     PKTIKVRELEAATLPRVLTQVDLALINTNYALEAKLNPTKDALIIEGSDSPYVNILVA
                     RADNKDNAALQKLAKALNSPEVKAFILEKYQGAVVPAF"
BASE COUNT         1228 a         2614 c         2680 g         1634 t
ORIGIN      
        1 tgggcgggcg ctttctgtac tgggcggtgg tgctgtaccg tgacagcaaa ccgcatgcgg
       61 cgatcagcac cttcaagtac agcatctggt atcttttcgc gctgtttatc gccctgctgg
      121 tcgaccacta tctgctgtta aacctgtaat cgttggtgcc atgacccgaa cccagatcac
      181 cgtttttgtt ctcgttgcta tcgtcgccct ggtgctgggc ctgaccgtca acaaggtgct
      241 gaccagcaag ggtcagggtg atcccaccgt gctgctcgat gccggcatcg tgctactgcc
      301 acagagtcgc agcctgccgg agttgagtct gaccaatcag gatggccaga cagtggcggt
      361 cgatcagctc aaggaccagt ggagcctgct gtttttcggc tacaccttct gcccggacat
      421 ttgcccggcc acccttgccc agctgcgcca actgcaaggt cagctaccgc cggaaaccct
      481 ggccaagttg cgcatcgtgc tggtcacggt tgacccgaac cgcgatacgc cggagcaact
      541 gaagaagtac ctggattact tcgacgccgg ctttatcggc ctgactggcg agcaggcgac
      601 tctgcagaag ctcgccaacg gcgtaagcat tccgtatatc ccggctgata ccagcaagga
      661 aaactacacc gtcgatcaca gcggcaacct ggtgatcatc ggcccggacg gcaagcagcg
      721 cggctttatc cgcgcaccaa tcaacaacgc caagctggcc gcgcagttgc ccggcctgct
      781 tgatcgccta taacacgcta tttggcaaat agctggctaa tgtccttgaa cgccttgaac
      841 tccagggcgt tgccgcacgg gtcgagcagg aacaacgtgg cctgctcgcc gacctggccg
      901 gcgaagcgca catagggttc gatcacaaat tgcgtgccct tggcctgtag ccgcgccgcc
      961 agcgtttgcc aatcctccca gcccaacacc acaccgaaat gcggcaccgg cacgtcgtgg
     1021 ccatccactg gattgctgtg cgcggcttcc tgatgcgcca ttttcggcgc ttggtgaatc
     1081 accagctggt ggccgaagaa atcgaaatcc acccagtggt cgctgctgcg gccttcggca
     1141 cagccgaaca cctcgccgta gaagtgccgt gcggcagcca ggtcgtaaac gggaatagcc
     1201 aggtgaaagg gtgcgagagc catgctgaga ttccttgaaa gggcggtctg ctgattctca
     1261 gcaacagcga ttctgattaa aagcgaatat ccttgcttac aagcacaaac aaaattgatc
     1321 agtcgaggcg tcaatgctgc gcgaattgaa aacctttgtg gcggtcgcac gccacggcac
     1381 ctttgccgcg gccggtcagc aggttggcct gacccagtcg gcggtcagcg cgcagatgcg
     1441 cgtgctggag cagggcctgg gtgtgcgctt gttcgatcgc agcggccgcg ccgccgtgct
     1501 caatgtcgcg ggccggcatg ccttgcctct ggccgagcag atgctcgcgc tttacgggca
     1561 aatggcgcgc ccggcatccc tggccgagtg gcagggcgaa ctcaaggtcg gcgctatcgc
     1621 tagcgtgcag accgggctgc tgcccgaggc gctgccggcc tttcgccagg ccgcgcctcg
     1681 ggtcgagctg aagctggtac cgggggtgtc gctgcagttg ctgagccagg ttgacgccgg
     1741 tgagttggat ctggcgctgc tgatcaagcc gccgttcgat ttgcccaagg agctgctgca
     1801 agtcagcctg gcgcgcgaac ctttcgtgct gatcacgccg ctgacagtgg cgggcgacga
     1861 cccgctgcag atcctcgccg agcagccctt tgtgcgttat gaccgcagct ccttcggcgg
     1921 gcgtcaggtc agccagtttc tccgcgagca gcgcctgcag gtgcacgaag ccctggagct
     1981 ggatgaactg gatgccatcg tgcgcatggt cgagtgtggc ctgggcgtct cgctcatccc
     2041 ccgcgccggc ctatggctac agcgctcgag ccaactgcgg gtgatcgacc tgggcgcgct
     2101 gaccttccac cgcgagctgg tggccctgct gcgccgggct cagcaacagc ccgcgctgga
     2161 gtgtttgctg cagtgtctac aggctcagaa cgctataggc gtccgctcaa ctgcaccacc
     2221 agtttgagct gctggccgtc gttctcgccc tgggccattg attaggaggg agcgaggcct
     2281 aggcagcgac ggcaagcagg gtcaggcgct tgaccattac tccaggtggc agccggctga
     2341 gcccggcggt ttctatggct ggaagggttt tttcgcgcag tgttccagcg taattgacta
     2401 gcaatgttat cttataacgt attagtattt gccccccctg ttttgctggc aggttttgcc
     2461 tgctgcattt tttgttcatc ctgctgctgg aaacgcccgt gagagccctg ctatatccgc
     2521 tgctggccat gctgttggct ggctgctcgc ccaaccagga cccgaaccag ctgtcgattg
     2581 gcggtccgtt tgaattcacc agccaggacc cgtcgcgcga cggttacctc tatacccgcc
     2641 tgcaggtagc cgaaaccctg cttgatgtag acgcgcaggg tcagttactg ccaggcctgg
     2701 ccgaggcctg gtcacagtct gaagacagcc gggtgtggcg cttcacgctg cgccaggggg
     2761 tgcgttttca tgatggtgcg ccgctggatg cggcggcggt ggtgcatgct ctgcaggtgg
     2821 cgcgcagtaa acccggtgtg ctcgcaaccg caccggttga ggccttcgag gccgaggatg
     2881 cgcacaccct gcggatccgt ctgagccgtc cctacaaccc gcttggcgcg gtgctggcgc
     2941 attacacgac ggtgatcctc tcgccggctt cttatgatgc caagggcctg gtcacccgct
     3001 tgcacggcac cgggccctat caggtcgagc gtttcgatcc accgcatcgt ctgggtgtgg
     3061 tgcgtttcgc cgattactgg ggcacgccag cgaccatcga gcgcgccgtc tacctcaccg
     3121 ggcaccgtgc ggaaagccgt gcgctgcagg tgatggccgg gcaaaccgat atcgtctaca
     3181 ccctcgaccc ggccagcctc gacctgctgg gtcgacgcga caatgtgcgg ctgcactcgc
     3241 acaccattcc gcgcaccatc cagctcaagc tcaacctagc tgatccgctg ctcgccgagc
     3301 gtgatacgcg cctggccctg agcctggcga tcgaccgcag cggcctggcc aacggggtgc
     3361 tgcgcgtacc cggcggggag gccgggcagt tggtgccgcc gtcgctcggc gactggcatc
     3421 tggccgatct acccgcgccg caacgggact tgttccgggc ccgtgcgctc ttggccgagc
     3481 aaggctggca ggccggtgcc gacggtatct tgcagcgcaa tggcgaggcg ttccgtctgc
     3541 gcctgatgac ctacgcagac cgtcccgaac tgtttcagct ggccaccgcg atccaggcgc
     3601 aatggcgcga gctgggcgtc gatttgcagg tctcggtggt caactccagc gatattcccg
     3661 ccgggcacca caacggcacg ctgcaggtgg cgctgatcgc ccgtaactac ggcaatatcg
     3721 ccgatccgct ggggctgctg ctggccgact acggtgatgg cggcaacggt gactggggcg
     3781 ccatggcgtg gcacaacagt gagttgccgg tgttgctgga ggcgcaacag agcgagcgcg
     3841 atgctggccg ctaccacgca cgcgcccagc gtgtggcgca gatcctggcc gaagaactgc
     3901 cgctgattcc ggtgctctat tacacccagc aggtcgccgt ctccgcccgc gtacaggggt
     3961 tcagtttcga cccttacgaa cgtaactacc ggattgccga gatgagtttt tttccatgct
     4021 gcaattgatc ctgcgtcgcc tgctgcaggc ggctttcgtc acctggagcg tgggcaccct
     4081 gagcttcgtg ctgatgcgtc tgctgcctgg tgatatggag tttcgtatcg ccgccgggcg
     4141 ctacggctat gaccacgtcg acctggccgc cgccgaggcg gtacgcgccg agctcggcct
     4201 ggaccgtcct ggttgggagg tttatctgca ctggttgggc gacctactgc gcttcaacct
     4261 gggcgattcg ctggtcagcg gcctgccggt gagccacgaa atcgcccacc tgctcggcca
     4321 taccctgttg ctggctggcg cggcgatgct catcgctttg ttgatcgccg cccctgtcgg
     4381 ggtctgggct gcgctgcgcg ccggcggctg gttcgaccgc ttgagcttgc tggtctcggc
     4441 cctgctgcgc gcccagccgg tgttcgtcat cggtctgggc ctgctgctgg tgttcgccct
     4501 ggagtggcgc atgctgccgg tggccggctt cgacggcccg cgctacctga tcctgccggc
     4561 gctgaccctg gccctggcga tggcggcggt gtccagccgg gtggtgcgcc acagcgccgc
     4621 cgaggtgctg cgctcggcct acttcaattt cgcccgggtc aagggcctga gcctgcgcca
     4681 ggctttgcag cggcatggcg cgcgcaatgc ggcgctgccg gtggtggtgt ttctcggcat
     4741 ccagctggcc agcgtgatcg aaggtgtggt gatgatcgag tcgctgttct cctggccggg
     4801 tgtcggccat ggcctggccc acgccgtgtt caaccgtgat gtgccgatga tccagggtgc
     4861 tgccatggcc ctgggcctgc tgttcgtggc cctcaacctg ctggtcgacc tggcctgcta
     4921 cctgatcgac ccccgtcagc ggagcgccca ctgatgctgc gtctattcgc ctccctgtct
     4981 accagccagc gcgtcggcgc cggcctgctc gccctgctgg tgttgttcgc cgtactcgaa
     5041 ggtctgctcg gccacaccga ccctgcgctg cagaacctgg cccagcgctt taccacgccg
     5101 agcctggccg aacccttggg caccgacccg ttcggccgca gcatgctggc gcgcatgggc
     5161 gccgcgttgc gcctgtcgct gagcctgagc ctggtctgcg tgctgacctc ggcgctgatt
     5221 ggcgtgggcc tcggcatcgc cgctggctgg cgccagggct gggtcgaccg ggtgctgtcg
     5281 ttcgtcctca atctggtgct ggcgctgccc ggcctggtgc tgatcctgct ggtcgcaggc
     5341 ctcgcgcccg gctcgctgct ggcgctgtat gtggccatct cgctggtgct gtgggtggag
     5401 tatttccgag tggtgcgggc gatcacccgg caggcggtgg cgtcgcagcc gatgcaggcc
     5461 tcgcgcatgc tcggcttcgg ctggcgttac tgtttgcgcc ggcatgtctg gccggccctg
     5521 gtcgggccgg tggcgaccct tgccgcgttt ggcgcggcgg gttcgatcat ggccctggcg
     5581 gccctcggct tcgttgccgt tggcctacgc ccgccgactg ccgagctggg tttgatgatg
     5641 gtcgagctat tcccttatta cagcgatgcc ccctgggtac tcgcacagcc tgtgctggcg
     5701 gtgtttctgc tggtgctggg cctcaacttg ctggcaggta aacgcgcatg accatcttgt
     5761 tgcgcattga agggctcagc gtccaggccg acggcaagac cttgctggaa cccttgagcc
     5821 tgaatctggc ggccggtgaa tgcctgacca tccttggtga aaccggcgcc ggaaagagcc
     5881 tgctgatgca cgccatcatg ggcaccctgc cgccaccact gacagccagc ggacgcatcc
     5941 tgctgggtga cagcgacctc gccagcctcg accgcagtgc tcgcgaacgg ctctggggcc
     6001 agcaattggc gatgttgccc caggaaccct ggcattccct tgacccgctg atgccggcac
     6061 tgcaacaggt ggccgaggtg cacgagtgtg tgcacggcca ggtgcccgag caggcccgac
     6121 aaaccgccac ccaggcgctc acggacgtgg gcctggaggc ggctgcggcg cgcttgccca
     6181 gccagttgtc cggcggcatg gcccagcgcc tggcgttttg cgccgccacg gcggcgggcg
     6241 cgccgattct gctcgccgat gagccgacca agggcctgga tgccagccgc cgtgatcagg
     6301 tcgccacgct gttgcgtcag catgcgcagc agggtgcgct gctgaccatc acccacgatg
     6361 tcgaagtggc gcggcaactg ggcggcacgt tgctggtgat gcgcgccggg cacctgctgg
     6421 agtcgggcga tgcccaggcg attctggacg cgccgcagca tgactacacc cgcgcgctga
     6481 tcgccgccga cccacgccac tggcccaccc cgctggtcgc cgccgtgacg ggcgcgccag
     6541 ctcacgagcc ggtgctggag gcgcgcggct tgtgcctcaa gcgcggtggg cggcagctgt
     6601 tcgagggtct ggatttacgc atcgaaccgg gagaaatcct cggcattgcc ggtgacagcg
     6661 gttgcggcaa gagcagcctg ggtgatctgc tgctcggcct gttgccggcc gatgccggca
     6721 tggtgacgcg gcgtccgggc gtggccgcgc atcgctacct caagctgtat caggacccgc
     6781 cggcagcctt cgccgccggg gtgccgctgc gccggctgtt cgacgacgtg ctgcgcctgc
     6841 accgccttga tcccgccaaa ctgcccccgc tgctggaccg cctgagcctg cgcccggaac
     6901 tgctggcgcg gccggcgacc gaagtctccg gcggtgaact gcagcgtttg gcgattgccc
     6961 gcgcactgct gctcgatccg ctgctgctgt tcgccgacga gccggtgtca cgcctggacc
     7021 cgattaccgc ccgcgaagtc accgagctgc tggtgcagag cgcccgtgag cgcggttgtg
     7081 cgctgttgct ggtgagccac gatgccgagg ccctggagcg cgcctgcgac cggcatattc
     7141 gtttgggtta atccatcctg acgaaaacgg ctgtaggtgc cctctttgct ctcacgcagg
     7201 ccaatcgggg taaaggctat gtcccaagta gggtgcgccg cgcgcaccga tgtcttgcag
     7261 ccagcgccgg tgcgcacggc gcaccctacg gttctaaaac caggtcatcg cccaagaaaa
     7321 accccgccaa ttggcggggt tcgtcgttgc aggcgggcga tcagaacgcc ggcaccaccg
     7381 caccctggta tttctccagg atgaaggctt tgacttcagg gctgttcagc gccttggcca
     7441 gcttctgcag ggccgcgttg tccttgttat cggcgcgcgc taccaggatg ttgacgtacg
     7501 gcgagtcgct gccttcgatg atcagggcgt ccttggtggg gttgagcttg gcttccaggg
     7561 cgtagttggt gttgatcagt gccaggtcaa cctgggtcag cacgcgcggc agggtggccg
     7621 cttccagttc acgcaccttg atggttttcg ggttctcgac gatgtctttt ggcgtggcga
     7681 ggataccggc ttccggcttg agggtgatga ggccagcctt ttgcagcagc agcagggcac
     7741 ggccgccatt ggtggcgtcg ttagggatca ccacgttggc gccttgcggc aggtcggcga
     7801 gggttttgtg ctggctggag taggcgccga agggctcgac gtgcacgccg gcaacgctga
     7861 ccagctcggt gccacggctc ttgttgaatt catccaggta cggctggtgc tggaagaagt
     7921 tggcatctag gcgctgctcg gcaacttgca cgttcggctg cacgtagtca gtgaagacct
     7981 tgatgttcag ctcaacgcct tctttggcca gggctggctt aacgaactcg aggatttccg
     8041 cgtgcggtac ggcagtggcc gcgacgctca ggctttcggc ctgggcggag aaggctgcca
     8101 gggcagcgaa tgcagcgatc agttttttca tcgtaaagct ccttgtggga tgtgtg
//
LOCUS       DASRUM010000008         2915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1174956, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000008 DASRUM010000000
VERSION     DASRUM010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174956"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1764
                     /locus_tag="VFF22_00530"
     CDS             22..1764
                     /locus_tag="VFF22_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX15342.1"
                     /translation="MFDNNNKTRHSMALAALLTLSGLSGAAWADAYEEAAKKWIGEEF
                     SPSTLTPEQQLEELKWFIKAAEPFRGMSISVASETIATHEYESKVLAKAFSEITGIKL
                     KHDLMQEGDVVEKLQTQMQSDKNIYDGYINDSDLIGTHFRYGKAVAISDMMEGEGKAV
                     TSPTLDLQDFIGISFTTGPDGKLYQLPDQQFANLYWFRADWFERPELKAKFKEIYGYE
                     LGVPVNWSAYEDIAEFFSEKVKEIDGQRIYGHMDYGKKDPSLGWRFTDAWFSMAGGGD
                     KGLPNGLPVDEWGIRVDGCRPVGSSVARGGDTNGPAAVYATTKYVDWMRKYAPPEAQG
                     MTFSESGPVPAQGSIAQQIFWYTAFTADMTKPGLPVVNEDGTPKWRMAPSPKGPYWEE
                     GMKLGYQDAGSWTFLKSTPEKQRLAAWLYAQFVTSKTVSLKKTLVGLTPIRESDINSQ
                     AMTDVAPKLGGLVEFYRSPARVQWTPTGTNVPDYPRLAQLWWQFIAEAASCDKSPQEA
                     LDGLAAAQDTMLGRLERSGVLGECGPKLNEPKDPQYWLDQPGAPKPKLANEKPKGETI
                     NYNELLKSWEQARD"
     gene            complement(1837..2691)
                     /gene="folD"
                     /locus_tag="VFF22_00535"
     CDS             complement(1837..2691)
                     /gene="folD"
                     /locus_tag="VFF22_00535"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HZX15343.1"
                     /translation="MTAQLIDGKAIAASLRQQIAQRVAERRQQGLRAPGLAVILVGSD
                     PASQVYVSHKRKDCEEVGFVSQAYDLPASTGQDELMALIDRLNDEPSIDGILVQLPLP
                     EHLDASLLLERIRPDKDVDGFHPYNIGRLAQRMPLLRPCTPKGIMTLLQSTGVDLYGM
                     HAVVVGASNIVGRPMAMELLLAGCTVTVTHRFTKDLPMHVGQADIVVVAAGKPGLVHG
                     EWIKPGAIVIDVGINRQTDGKLIGDVVYETALSRAGWITPVPGGVGPMTRACLLENTL
                     HAAEHLHD"
BASE COUNT          628 a          838 c          890 g          559 t
ORIGIN      
        1 aaccctgaga tggaggtctc tatgttcgac aataacaaca aaactcgaca tagcatggcg
       61 ctggctgccc tgctgacgtt gtccggcttg agcggcgcag cctgggctga tgcctacgaa
      121 gaagcggcaa agaagtggat cggcgaggag ttcagcccct cgaccctgac gcctgagcag
      181 caactcgaag agttgaagtg gtttatcaag gccgccgagc cgtttcgcgg catgagcatc
      241 agtgtggcct cggaaaccat cgccactcac gagtacgagt ccaaggtgct ggccaaggcc
      301 ttcagcgaaa tcactgggat caagctcaag cacgacctga tgcaggaagg cgatgtcgtc
      361 gagaaactgc aaacccagat gcagtcggac aagaacatct acgacggcta tatcaacgac
      421 tcggacctga tcggtacgca tttccgttat ggcaaggccg tggccatcag cgacatgatg
      481 gaaggcgaag gcaaggctgt gacctcgccg accctcgatc tgcaggactt tatcggcatt
      541 tccttcacca ccgggcccga cggcaagctc tatcagctgc cggaccagca gttcgccaac
      601 ctctactggt tccgcgccga ctggttcgag cgtccggagt tgaaggccaa gttcaaggaa
      661 atctacggct acgagctggg cgtaccggtc aactggtcgg cctatgaaga tatcgccgag
      721 ttcttctccg agaaggtcaa ggagatcgat ggccagcgca tctatggcca tatggattac
      781 ggcaagaagg acccatccct gggatggcgc tttaccgatg cctggttctc catggcgggt
      841 ggcggcgaca aggggctgcc taacggtttg cccgtggatg agtggggcat tcgcgtagat
      901 ggttgtcgtc cggtgggttc cagcgtcgcc cgtggcggcg ataccaatgg cccggcggcg
      961 gtgtatgcca ccaccaaata tgtcgactgg atgcgcaagt acgcgccgcc ggaagcccag
     1021 ggcatgacct tctccgagtc cggtccggtg ccagcgcagg gcagcatcgc ccagcagatc
     1081 ttctggtaca ccgcctttac cgccgacatg accaagccag gcttgccggt agtaaatgaa
     1141 gacggcacgc cgaagtggcg tatggcgcct tcaccgaaag gtccgtactg ggaagagggc
     1201 atgaagctgg gctatcagga cgccggttcc tggaccttcc tcaagtccac cccggagaaa
     1261 cagcgcctgg ccgcctggct gtatgcgcag ttcgttacct cgaaaaccgt atcgctgaag
     1321 aagaccctgg ttggcctgac gccgattcgt gagtccgata tcaactcgca ggccatgacc
     1381 gatgttgcgc cgaaactggg cggcctggtg gagttctatc gcagcccggc gcgcgtacag
     1441 tggaccccga ccggtaccaa cgtgcctgac tacccacgtc tggctcagct gtggtggcaa
     1501 ttcattgccg aagcagccag ctgcgataag tcgccacaag aggcgctgga tggcctggct
     1561 gcagcgcagg acaccatgct gggtcgcctt gagcgttccg gcgtgctcgg cgaatgtgga
     1621 ccgaagctga acgagccgaa agatccgcag tactggctgg atcaaccggg tgcaccgaag
     1681 ccgaaactgg ccaacgagaa gcccaagggt gaaaccatca actacaacga gctgctgaaa
     1741 tcctgggaac aagcccgcga ctgacgctgc gggttgagct caagcataaa aaaacggcac
     1801 ctaaggtgcc gttttctatt tctgctagcc ctgtacttag tcgtgcagat gctcggcggc
     1861 atgcagggtg ttttccagta ggcatgcacg ggtcatcggg ccaacgccgc ctggcaccgg
     1921 agtgatccaa ccggcgcggg acagggcggt ttcatacacc acatcgccaa tcagcttgcc
     1981 gtcggtctga cggttgatcc ccacatcaat cacgatggcg ccaggcttga tccactcgcc
     2041 gtgaaccagg ccaggcttgc cggccgcaac cacgacgata tcggcctggc cgacatgcat
     2101 gggcaggtct ttggtgaagc ggtgggtcac ggtgacggta cagccggcca gcagcagctc
     2161 catcgccatc ggccggccga cgatattcga ggcgccaacc accactgcat gcatgccata
     2221 caggtcaacg ccggtgcttt gcagtagggt catgatgcct ttcggggtgc aggggcgcag
     2281 cagtggcatg cgctgggcca ggcgaccgat gttgtagggg tgaaaaccat caacgtcttt
     2341 atccgggcga atgcgctcca gtaataaaga agcgtcgaga tgttctggca gtggcagctg
     2401 caccagaatg ccgtcgatgc tcggttcgtc attcaggcgg tcgatcaatg ccatcagctc
     2461 gtcctggccg gtgctggcag gcaggtcata ggcctgcgag acgaagccaa cttcttcgca
     2521 gtctttgcgt ttgtgcgaaa cgtagacctg ggacgcaggg tcgctaccga cgagaatcac
     2581 cgccagcccg ggtgcgcgca ggccttgctg acgtcgttcg gcgacacgtt gggcgatctg
     2641 ctggcgcagg ctggcggcaa ttgctttgcc atcgatcagt tgtgcggtca ttgcgcttgg
     2701 ttaaccataa aaaaaggatg gaaaaaggac gcgcattctc gcatgaccat gctgtggggc
     2761 aaaggcgcac gccgcggttt tcgcgtaact cctttaaatg gctgaatttt tttaactttt
     2821 cctgttgacg acctgaaacg gcctctataa cattcgcccc gcttgtcgag cacagccagt
     2881 cgctgggtaa gacggcaaag gtcgaagctg caaag
//
LOCUS       DASRUM010000009        17028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2345687, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000009 DASRUM010000000
VERSION     DASRUM010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 17028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17028
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2345687"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..2052)
                     /gene="tkt"
                     /locus_tag="VFF22_00540"
     CDS             complement(52..2052)
                     /gene="tkt"
                     /locus_tag="VFF22_00540"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZX15344.1"
                     /translation="MPSRRERANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWRDF
                     LKHNPSNPKFADRDRFVLSNGHGSMLIYSLLHLTGYDLGIEDLKNFRQLHSRTPGHPE
                     YGYTPGVETTTGPLGQGIANAVGFAIAEKVLGAQFNRDGHQIVDHFTYAFLGDGCMME
                     GISHEVCSLAGTLGLGKLVAFYDDNGISIDGEVHGWFTDDTPARFEAYGWQVIRNVDG
                     HDADEIKIAIETARKTSDRPTLICCKTTIGFGSPNKQGKEECHGAPLGNDEIALTRAT
                     LGWNHGPFEIPAEIYAEWDAKAAGAAAEAEWGQRFAAYAAAYPELASEFKRRVAGELP
                     ADFAAKAAAYIQDVAEKGETIASRKASQNALNAFGPLLPEFLGGSADLAGSNLTLWKG
                     CKGVSADDASGNYMFYGVREFGMSAIMNGVALHGGFVPYGATFLIFMEYARNAVRMAS
                     LMKQRVLFVFTHDSIGLGEDGPTHQPIEQLASLRGTPNLDTWRPCDAVESAVAWKYAI
                     ERNDGPSALIFSRQNLPHQSRSAQQLADVARGGYVLKDCAGEPELILIATGSEIGLAV
                     SAFEKLSEQGRKVRVVSMPSTSVFDQQDAGYKQAVLPVQVGSRIAIEASHADYWYKYV
                     GLEGRIIGMTTFGESAPAPALFEEFGFTVENILETAAELLDA"
     gene            2250..3281
                     /locus_tag="VFF22_00545"
     CDS             2250..3281
                     /locus_tag="VFF22_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZX15345.1"
                     /translation="MNMRASHLDFEPVDALAALCKASGDALRLNILRALANDSFGVLE
                     LAQIFATGQSGISHHLKVLAQAGLVATRREGNAIFYRRALAQGDQLGSRLHAALLEEV
                     DQLPLPAEVQGRIAAVHGQRAAASQEFFARSAASFQAQQDLIAGLPQYRDSVLALLDS
                     LGFASDACAVEIGPGDGGFLPELARRFQNVLALDNSAAMLELARMRCTEEGLNNVELQ
                     LADALHDDYPAADCLVLNMVLHHFAAPADALKQLAQRVKPGGSLLITELCSHNQSWAR
                     EACGDLWLGFEQDDLARWAVAAGLNTCESLYVGLRNGFQIQVRHFAKPSVTIGLRAAI
                     TGKQQETER"
     gene            3278..4468
                     /gene="metK"
                     /locus_tag="VFF22_00550"
     CDS             3278..4468
                     /gene="metK"
                     /locus_tag="VFF22_00550"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZX15346.1"
                     /translation="MSEYSLFTSESVSEGHPDKIADQISDAVLDAIIAQDKHARVAVE
                     TLVKTGVAIVAGEVTTSAWVDLEQIVRDVICNIGYTNSDVGFDGATCGVLNIIGKQSP
                     DINQGVDRAKPEDQGAGDQGLMFGYASNETYVLMPAPICFSHRLVERQAEARKSGLLP
                     WLRPDAKSQVTCQYENGKVVGIDAVVLSTQHNPEVSLADLREGVMELIIKHALPAELL
                     HKGTQFHINPTGNFVIGGPVGDCGLTGRKIIVDSYGGMARHGGGAFSGKDPSKVDRSA
                     AYAGRYVAKNIVAAGLAERCEIQVSYAIGVAQPTSISINTFGTNKVADDLIIKLVREV
                     FDLRPYAITKMLDLLHPMYQETAAYGHFGRTPKQKTVGDDSFTTFTWEKTDKVADLRA
                     AAGL"
     gene            complement(4539..5447)
                     /locus_tag="VFF22_00555"
     CDS             complement(4539..5447)
                     /locus_tag="VFF22_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15347.1"
                     /translation="MGLLRLGRVCLCLLVAGLAPFAAVHGAEDVAPQWRFIHNAPETE
                     GDQRYAYHWRVLRAALEVTRSKYGDYRLEPGQPMSEKLQVVEMQRPQVGLNTLVLDAT
                     QTLEEVLLPVKIPVDKGLLGYRVFLIQAEDQPRFAGVQSLEQLRQLRFGQQREWSDVA
                     VYQAAGLPVVTGSSYEGLFHMLMLRRFDAFGRGVSEVNGELAHWRKQYPQMTIENELL
                     LYYPLPVYFWFARTDEGRLRAQRVEEGMQTLMADGTLDRLFSEEYAATIQQLGLDSRR
                     TLKIANPHLSSNHPFDKPEYWFVPTP"
     gene            5538..7211
                     /gene="ligB"
                     /locus_tag="VFF22_00560"
     CDS             5538..7211
                     /gene="ligB"
                     /locus_tag="VFF22_00560"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HZX15348.1"
                     /translation="MKAAIALLFTALTLDTWAAPCPDWPAQRAKLEIAALSQQLAAWD
                     DVYHRQGESPVADELYDQASANLQRWRACFPEASVPPSDPLSTSSGRHHHPTAHTGLR
                     KLADSQAAGAWISGREDLWIQPKVDGVAVSLIYRDGQLQQLISRGDGRSGQDWSAHAR
                     HLQAIPRQLPKPFNLALQGELYWRLPGHIQHKSGGIGARSKVAGLMNRQTLTAEDAAG
                     IGLFVWDWPDGPALMPQRLQQLAELGFAESQQFSQPIRSLEQAAHWRERWYNSALPFA
                     TDGVVLRQGQRPAAARWQAEPPHWAVAWKYPVSQALAVVQAVDFRIGRSGRITPVLRV
                     QPVKLDDRRIEYVSAGSLQRWQALDIRPGDQVVIRLSGLTIPVLDSVLWRTQQRAALQ
                     VPDAAAYHPLSCWRATPACASQFRARLAWLSSKQGLALPGVGPGTWEKLLHAQRLNSL
                     LDWLHLSNEQLSSVPGLGPRSSAALQQSFRLARERPMQDWLRALGLPISGNLALDADW
                     DTLAQRSVADWQRHAGIGPQRAAQLRAFFQHPEVQALRQQLRTAQIAGF"
     gene            7275..7664
                     /locus_tag="VFF22_00565"
     CDS             7275..7664
                     /locus_tag="VFF22_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017735695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HZX15349.1"
                     /translation="MHKALLLSSLLLAATLATAATTQLTQLTGCAAKRQTISEQIEAA
                     KSHGNSSQQAGLEKALKEVENNCNDASLRAEREAKVVEAEDEVSEREGDLREALADGD
                     QDDISKRQHKLEEARAELQQAREELSK"
     gene            complement(7670..8119)
                     /locus_tag="VFF22_00570"
     CDS             complement(7670..8119)
                     /locus_tag="VFF22_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZX15350.1"
                     /translation="MKLKTLSLVVLALAASGCGGVDPNSPQGQRQSIFKQMLKVSEDL
                     GGMLRGRLAFKEQAFIDGAAKLDQLTRTPWQHFPQVRDEGEESRAKDDVWQRQARFQE
                     LAQAMEASTAALVAATAAQPLTPEALTAPMQRVEDSCKKCHEEFRAF"
     gene            complement(8243..8629)
                     /locus_tag="VFF22_00575"
     CDS             complement(8243..8629)
                     /locus_tag="VFF22_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003157091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HZX15351.1"
                     /translation="MTLAYWCVLIAIVLPYLSTAAAKFLGPGYGPRANQDPRAFLSTL
                     EGWRKRANNAQLNGFEVTPAFAAAVIIAHQAGGAEQGLLDQLAIAFVLSRVLYFLCYL
                     ADWGPIRSLVWFAGMALIVSLFVVSA"
     gene            complement(8727..10010)
                     /locus_tag="VFF22_00580"
     CDS             complement(8727..10010)
                     /locus_tag="VFF22_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HZX15352.1"
                     /translation="MNTAIEVLQSSQPHINSERLWQSLMELAQLGATVKGGVCRLALT
                     DLDRQARDLFVRWCEEAGCTVTVDGVGNIFARRPGHNAALPPVMTGSHIDTQPTGGKF
                     DGCFGVLAGVEVLRTLNDLGVQTEAPLEVVVWTNEEGSRFAPCMMGSGVFAEKFTLEE
                     TLAKRDAEGVSVGEALNAIGYSGSRAVSGHAVGAYFEAHIEQGPILEDQGKTIGVVLG
                     ALGQKWFDLNLKGVEAHAGPTPMHLRKDALVGASAVVAAVNRVALEHQPHACGTVGCL
                     QAYPGSRNVIPGEVRMTLDFRHLEPARLDSMIAEVRGVIEATCAKHGLSFELMPTADF
                     PPLYFDQGCVEAVRGAAAGLGLSHMDIVSGAGHDAIFLAELGPAGMIFVPCEGGISHN
                     EIENAAPADLAAGCAVLLRAMLAASAALAKGKLAA"
     gene            complement(10169..11656)
                     /locus_tag="VFF22_00585"
     CDS             complement(10169..11656)
                     /locus_tag="VFF22_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HZX15353.1"
                     /translation="MQQSRSQVTERDGLYELAAGPDVLDSPRYNHDIAPTKVHERTWN
                     KWHITALWVGMSICVPTYTLGGVLTAYFGLSVGEALLAILLANIIVLIPLTLNAFPGT
                     KYGIPFPVLLRSSFGVIGSNVPCLIRAVVACGWFGIQTMFGGLAIHLLLGSIFEGWKS
                     LGGTGEVIGFMIFWVLNLWVVLRGAESIKWLETLSAPLLVLVGAGLLVWALPNVSMSE
                     LLAQPANRPEGASVTSYFLAGLTAMVGFWATLSLNIPDFSRYAKSQKDQIIGQVIGLP
                     LTMFLFAALGVVMTAASASLVGETVSDPVTLIGHIQSPGWVALAMALIIVATLSTNTA
                     ANIVSPTNDFQNIAPKLINRTKAVILTGLIGLGLMGHELLKKLGLLVSDLSLESVYSN
                     WLLGYSSLLGPIAGIMVVDYFLIKKQRLDLAGLYRDDVYPAWNWIGFVAFAVPVALTL
                     LSLGSSAFSWFYDYGWFTGSFLGGLIYFGLCSLRSAQPVAAKAPV"
     gene            12294..13733
                     /gene="hydA"
                     /locus_tag="VFF22_00590"
     CDS             12294..13733
                     /gene="hydA"
                     /locus_tag="VFF22_00590"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HZX15354.1"
                     /translation="MSLLIRGATLVTHEESYRADVLCADGLIQAIGDNLDAPAGCQVL
                     DGSGQYLMPGGIDPHTHMQLPFMGTVASEDFFSGTAAGLAGGTTSIIDFVIPNPQQSL
                     LEAFHTWRGWAEKSASDYGFHVAITWWSDEVSREMGELVAKFGVNSFKHFMAYKNAIM
                     AADDTLVASFERCLELGAVPTVHAENGELVYHLQQKLLAQGLTGPEAHPLSRPSQVEG
                     EAASRAIRIAETLGTPLYLVHVSTKEALDEITYARAKGQPVYGEVLAGHLLLDDSVYR
                     HPDWQTAAGYVMSPPFRPLGHQEALWHGLQSGNLHTTATDHCCFCAEQKAAGRDDFSK
                     IPNGTAGIEDRMAVLWDEGVNSGKLSINDFVALTSTNTAKIFNLFPRKGALRVGADAD
                     LVLWDPQGTRTISAKTHHQQVDFNIFEGKTVRGVPSHTISQGKLVWVDGDLRAERGAG
                     RYVERPAYPAVFDLLSKRAEQNRPTAVER"
     gene            13934..15301
                     /locus_tag="VFF22_00595"
     CDS             13934..15301
                     /locus_tag="VFF22_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZX15355.1"
                     /translation="MIDTLSHLPRPDAGAAELADRFSDLAPPLTARQAALESSRCLYC
                     YDAPCVNACPSEIDIPSFIRNISHENVQGAAEKILSANILGGSCARVCPTEILCQQAC
                     VRNNAQECAPVLIGLLQRYAIDNAQFTEHPFQRAPASGKRIAMVGAGPAGLSCAHRLA
                     MHGHEVVIFEAREKAGGLNEYGIAAYKLVDDFAQREMEFLLQIGGIEIRHGHKLGDDL
                     SLSELHQQFDAVFLGIGLAASKRLGLPGEDAPGLLAATDYIRELRQSDDLTQLPVAER
                     CIVLGAGNTAIDMAVQMSKLGARDVNLVYRRGFEEMGATEHEQDIAKANQVRLLTWAQ
                     PDAVLLNEQGQVRGMRFARTRVENGRLQSTGQTFELAADAIFTAIGQAFDGSALSDPL
                     ARELKREGERIWVDAQLQTSVPGVYAGGDCTALGQDLTVQAVQHGKLAAEAIHSHLML
                     NVEAA"
     gene            15302..16576
                     /gene="preA"
                     /locus_tag="VFF22_00600"
     CDS             15302..16576
                     /gene="preA"
                     /locus_tag="VFF22_00600"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HZX15356.1"
                     /translation="MADLSIEFAGIKAPNPFWLASAPPTDKAYNVVRAFEAGWGGVVW
                     KTLGEDPAAVNVSSRYSAHFGSNRQVQGINNIELITDRSLEINLREITQVKKDWPDRA
                     LIVSLMVPCVEESWKAILPLVEATGCDGIELNFGCPHGMPERGMGAAVGQVPEYVEMV
                     TRWCKTYSSLPVIVKLTPNITDVRLSARAAHRGGADAVSLINTINSITSVDLERMVAH
                     PIVGTQSTHGGYCGSAVKPIALNMVAEIARDPKTLGLPICGIGGIGSWRDAAEFVALG
                     CGAVQVCTAAMLHGFRIVEEMKDGLSRWMDSQGYQSLADFSGRAVGNTTDWKYLDINY
                     QVIAKIDQDACIGCGRCHIACEDTSHQAIASLTQTDGTHKYEVIDEECVGCNLCQITC
                     PVENCIEMVPQDTGKPFLDWNHDPRNPYRVAS"
     gene            complement(16680..>17028)
                     /locus_tag="VFF22_00605"
     CDS             complement(16680..>17028)
                     /locus_tag="VFF22_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZX15357.1"
                     /translation="RAKMNHSRSRPHGSKVWADEIIHGAPMLGASLDDSLYSWAKMKE
                     AKIRQWIEDKRILAVEPSALLYMIWASTQHYADFSHQVAILNDHQALSELQFERAVQT
                     VTSVILRGIGLEP"
BASE COUNT         3423 a         5555 c         5110 g         2940 t
ORIGIN      
        1 ctcgcgatca atgtttgagc atcgcgagca agctcgctcc taccaaggcc ttcaggcgtc
       61 cagcagttcg gctgcagttt ccaggatatt ttcgacggtg aagccgaatt cctcgaatag
      121 cgccggggcc ggggcggact caccgaaggt ggtcatgcca ataatgcggc cttccagacc
      181 gacgtatttg taccagtagt cagcatgcga ggcctcgatg gcgatccgcg aacccacttg
      241 caccggcagc acggcctgct tgtagccggc atcctgctgg tcgaacacgc tggtggacgg
      301 catcgacaca acgcgcacct tgcggccttg ctcgctgagc ttctcaaacg cagatacggc
      361 caaaccgatt tccgaaccgg tggcgatcag gatcagctcc ggctcgccgg cgcagtcctt
      421 caatacatag ccaccgcggg cgacatcggc cagctgctgg gcgctgcgcg actggtgcgg
      481 caggttctgc cggctgaaga tcagcgcaga cggaccgtcg ttacgctcga tggcgtactt
      541 ccaggccacc gccgactcca cggcgtcgca tgggcgccag gtgtcgaggt tcggcgtgcc
      601 gcgcaggctg gccagttgct cgattggctg gtgggtcggg ccgtcttcac ccagaccgat
      661 ggagtcgtgg gtaaacacga acagcacacg ctgtttcatc agcgaggcca tgcgcacggc
      721 gttgcgcgcg tactccataa agatcaggaa ggtcgcgccg tacggcacga aaccaccgtg
      781 cagggccacg ccgttcatga tcgcgctcat gccgaactcg cgcacgccgt agaacatata
      841 gttgccggaa gcatcgtcag ccgacacgcc cttgcagccc ttccacaggg ttaggttgga
      901 gccggccaga tccgccgagc caccgaggaa ttccggcagc agtgggccaa aggcgttcag
      961 cgcgttctgg ctggccttac ggctagcgat ggtttcgccc ttctcggcga cgtcctggat
     1021 ataggccgcg gctttggcgg cgaaatcagc gggcagctca ccggcgacgc ggcgtttgaa
     1081 ctcgctggcc aattcaggat aagcggcggc gtaggcagcg aaacgctgac cccactcggc
     1141 ttcggcggcg gcgccggcgg ccttggcgtc ccactcggcg tagatctcgg cggggatttc
     1201 gaaggggcca tggttccagc ccagggtggc gcgggtcagg gcgatttcgt cattgcccag
     1261 cggcgcgccg tggcactctt ccttgccctg cttgttcggc gagccgaagc cgatggtggt
     1321 tttgcagcag atcagggtcg gacggtcgct ggttttgcgc gcggtctcga tggcgatctt
     1381 gatttcgtca gcgtcgtggc catcgacatt gcgaatcacc tgccagccat aagcttcgaa
     1441 gcgcgcaggg gtgtcgtcgg taaaccagcc atgcacttcg ccgtcgatgg agataccgtt
     1501 gtcgtcataa aacgccacta gcttgcccag gcccagggtg ccggccagcg agcagacttc
     1561 atggctgatg ccttccatca tgcaaccgtc gccgaggaaa gcgtaggtga agtggtcaac
     1621 gatctggtgg ccgtcgcggt tgaactgcgc gcccagcact ttctcggcga tggcgaaacc
     1681 gacggcgttg gcgatgccct ggcccagcgg gccggtggtg gtttccacgc ctggggtgta
     1741 gccatattca gggtggcctg gggtacggct gtgcagttga cggaagtttt tcaggtcttc
     1801 gatgcccagg tcgtagccgg tgaggtgcag caacgagtag atcagcatcg agccgtggcc
     1861 gttggacagc acaaagcggt cacggtcggc gaatttcggg ttgctcgggt tgtgcttgag
     1921 gaaatcacgc cacagtactt cggcgatatc cgccataccc atcggggcac cggggtggcc
     1981 gctgttggct ttctgcacgg catccatgct cagggcacga atggcattgg ctcgctcacg
     2041 acggctgggc atcactgaat ctcctacggg ctgcttgggc aactgggggt cgaaaaaggc
     2101 ggccattttc gcccagcggg gcctgtcggg gcaatgacag atagtcgcac agccaatgtt
     2161 ttctggcctg agctccagca agggcaatat caaaactttt tgatattgcc cttgctggat
     2221 aaaaagtgac cgactagact gcccagccta tgaatatgcg cgcctcccat ctggactttg
     2281 agccggtcga cgccctcgca gccctgtgta aggccagcgg tgatgcgctg cgcctgaata
     2341 ttctgcgcgc cctggccaac gactcgttcg gcgtactgga attggcgcag atcttcgcca
     2401 ccggtcaatc cggtatcagc catcacctca aggtgctggc ccaggccggg ctggtagcaa
     2461 cgcgccgcga aggcaacgcg attttctacc gccgcgccct ggcccagggc gaccagctgg
     2521 gcagtcgcct gcatgctgcg ctgctagagg aagtcgacca gttgccactg ccggccgagg
     2581 tacagggacg cattgccgcc gtacacggcc aacgtgccgc tgccagccag gaattcttcg
     2641 cccgcagcgc ggcgagcttc caggcgcagc aggatctgat cgccggcctg ccgcaatacc
     2701 gcgacagcgt gctggccctg ctcgactccc taggctttgc cagtgacgcc tgcgccgtgg
     2761 agatcggccc tggcgatggc ggctttctgc cggaactggc gcggcgcttc cagaatgtgc
     2821 tggctctgga caacagtgcg gcgatgctcg aactggcgcg tatgcgctgc accgaggaag
     2881 gcctaaataa tgttgagctg cagctggccg acgccctgca cgacgactac ccggctgccg
     2941 actgcctcgt gctgaacatg gtgctgcacc acttcgccgc cccggccgat gcgctcaaac
     3001 agctggcgca gcgcgttaaa cctggcggca gcctgctgat caccgaactg tgcagccaca
     3061 accagagctg ggcccgcgaa gcctgcggtg acctctggct gggcttcgaa caagacgacc
     3121 tggcccgctg ggccgtcgct gctggtctca acacctgcga aagcctctat gtcggcttgc
     3181 gcaatggctt ccagattcag gttcggcact ttgccaaacc ttcagtaacc attggcctac
     3241 gggctgcaat taccggtaaa caacaggaaa ccgaacgatg agcgaatact ccctctttac
     3301 ctccgagtcc gtctccgaag gccatcccga caaaatcgcc gaccagatct ccgatgcggt
     3361 gctggacgcc atcatcgccc aggacaaaca cgcccgtgtg gccgtggaaa ccctggtcaa
     3421 gaccggcgtg gccatcgttg ccggtgaagt caccaccagc gcctgggtcg atctggagca
     3481 gatcgtccgc gacgtgatct gcaacatcgg ctacaccaac tccgacgtcg gcttcgatgg
     3541 cgccacctgc ggcgtgctga atatcattgg caagcagtcc cctgacatca accagggcgt
     3601 tgaccgcgcc aaacctgaag atcagggtgc cggcgaccag ggcctgatgt tcggctatgc
     3661 cagcaacgaa acctacgtgc tgatgcccgc accgatctgc ttcagccacc gcttggtcga
     3721 gcgccaggcc gaagcgcgca agtccggcct gctgccgtgg ctgcgcccgg atgcgaaatc
     3781 ccaggtcacc tgccagtacg aaaacggcaa ggtagtcggc atcgacgccg tggtgctgtc
     3841 cactcagcac aacccggaag tgtcgctggc tgacctgcgc gaaggcgtga tggagctgat
     3901 catcaagcac gccctgccgg ccgagctgct gcacaaaggc acgcagttcc acatcaaccc
     3961 aaccggcaac ttcgtcattg gcggcccggt gggcgactgc ggcctgaccg ggcgcaagat
     4021 tatcgtcgac agctacggcg gcatggcccg tcacggcggc ggcgcgttct ccggcaagga
     4081 cccatccaag gttgaccgtt cggccgccta cgctggtcgt tatgtggcga agaacatcgt
     4141 tgccgctggt ctggctgagc gctgcgaaat ccaggtgtcc tacgccatcg gcgtggccca
     4201 gccgacctcg atctcgatca acaccttcgg caccaacaag gtggcggacg acttgatcat
     4261 caagctggtg cgcgaagtat tcgacctgcg cccttacgcc atcaccaaga tgcttgacct
     4321 gctgcacccg atgtaccagg aaaccgcggc ctacggccac ttcggccgca cgccgaaaca
     4381 gaagaccgtc ggcgacgaca gcttcaccac cttcacctgg gaaaagaccg acaaggtcgc
     4441 cgacctgcgc gctgccgccg gcctgtaagc ccaaattggc tgcagaaaaa acccgctacg
     4501 gcgggttttt tattgcgatc aactagcgct tcaccacgct agggtgttgg cacaaaccaa
     4561 tattccggtt tgtcgaaggg atggttcgag gagagatgag gattggcaat cttcaaggtg
     4621 cgccgactat ccaaaccaag ctgctggatg gtggcggcat actcttcgct aaacaaacga
     4681 tccagagtgc cgtcggccat cagcgtttgc atgccctcct cgacacgctg cgcgcgcagc
     4741 cgcccctcgt cggtgcgggc aaaccagaag tacaccggca acgggtaata cagcagcaac
     4801 tcgttctcga tggtcatctg cgggtattgc ttgcgccaat gcgccagctc gccgttcacc
     4861 tcgctcacgc cacgaccgaa ggcatcgaaa cgccgcagca tcagcatatg aaacaaacct
     4921 tcatagctgc tgccggtgac caccggcaat ccagccgcct gatacaccgc cacatcggac
     4981 cattcacgct gctgaccaaa ccgcagctgg cgcaactgct caagcgactg cacgccagca
     5041 aagcgcggtt gatcctcggc ctgaatcagg aatacccgat aaccgagcaa gcccttgtcg
     5101 accggaatct ttaccggcag caggacttct tccagggtct gcgtggcatc cagcaccagg
     5161 gtattaaggc caacctgcgg gcgctgcatc tcgaccacct gcaacttctc gctcatcggc
     5221 tggccaggct caagccgata atcgccgtat ttgctgcggg taacctccag cgccgcccgc
     5281 aatacgcgcc agtgataggc gtagcgctgg tcgccctcgg tttccggggc gttgtggata
     5341 aagcgccact gcggcgcaac atcctcggcc ccatgcaccg cagcaaaggg tgcaaggcct
     5401 gcgacaagca ggcacaggca aacacggcca agtcgaagca accccattgg cataaagccc
     5461 ccacacaaac gccagaactg acccatcaag catagctgct acgctgaaaa aaccgcttcc
     5521 gagcaaggac gctcagcatg aaagccgcca tcgcgcttct ctttaccgcc ctgaccctcg
     5581 acacttgggc cgcgccctgc cccgactggc cggcgcaacg cgccaagctg gaaatcgccg
     5641 cgctgagcca gcaactggcg gcctgggacg acgtctacca tcgccagggc gaatcaccgg
     5701 tggcggacga actctatgac caggccagcg ccaatctgca gcgctggcgt gcctgcttcc
     5761 ccgaagcatc agttccccct agcgatccgc tcagcaccag cagcggccgc catcaccacc
     5821 caaccgccca taccggcctg cgcaaactgg ccgatagcca agcggcaggg gcctggattt
     5881 ctggccggga ggatttatgg attcagccca aggtcgatgg cgttgccgtc agcctgatct
     5941 atcgcgacgg ccagctgcag cagttgatca gccgtggtga tggccgtagc ggccaggact
     6001 ggagtgccca cgcccgccat ctgcaggcca ttcccagaca actgcccaag ccgtttaatt
     6061 tggcgctgca gggcgagttg tactggcgat tgccggggca tatccagcac aagtccggcg
     6121 gcatcggcgc acgcagcaag gtagccgggc tgatgaacag acagacgctg accgccgagg
     6181 acgccgccgg catcggtcta tttgtctggg attggcccga cggcccggca ttgatgcccc
     6241 agcgcttgca acaactggcg gagctgggat ttgccgagag ccagcagttc agccaaccga
     6301 tccgcagcct ggagcaggcc gcccactggc gcgaacgctg gtacaacagc gccctgccct
     6361 tcgccaccga tggcgtggtc ctgcgccaag ggcaacgccc ggcggccgca cgctggcaag
     6421 ccgaaccgcc gcattgggcg gtggcctgga agtatccggt gagccaggcc ttggccgtgg
     6481 tgcaggcggt ggattttcgt atcggccgca gcgggcgcat caccccggtg ctgcgcgtac
     6541 agccggtcaa gctggatgac aggcgcatcg aatatgtcag cgccggctcg ctgcagcgct
     6601 ggcaggcctt ggatattcga cccggcgatc aggtggtgat ccgcctttcc ggcctgacca
     6661 ttccggtgct cgacagcgtc ctctggcgaa cccagcaacg cgccgcactg caagtgcctg
     6721 acgcggcggc ctatcaccct ttgagctgct ggcgcgccac cccggcctgc gccagccagt
     6781 tccgtgcgcg cctggcctgg cttagcagca aacagggcct ggccttaccg ggcgtaggcc
     6841 ccggcacctg ggaaaaactg ctgcatgcgc aacgcctgaa cagcctgctc gactggttgc
     6901 acctctcaaa cgagcagctc agcagcgtgc caggcctcgg cccacgcagc tcagccgcgc
     6961 tgcagcaaag cttccgcctg gcccgcgaac ggcccatgca ggactggctg cgcgccctcg
     7021 gcctgccgat cagtggcaac ctggcgctgg acgccgactg ggacaccctg gcgcaacgca
     7081 gcgtggccga ctggcaacgt cacgccggta tcggcccaca gcgagcggcg cagttacggg
     7141 ccttctttca acacccggaa gtgcaggcgc tgcggcaaca actacgtacc gcgcagatag
     7201 ccgggttttg agcagaaata aactttgccc aacgcgagcg gtcgactgca cgcaaccatc
     7261 actcaggaac caccatgcac aaagcactac tactgagcag cctgctgctg gctgccacac
     7321 tggcgactgc cgccaccaca cagctcaccc aacttaccgg ctgcgccgcc aagcgccaga
     7381 ccatcagcga gcaaatcgag gcagccaaaa gccacggcaa cagcagccag caagccggcc
     7441 tggaaaaagc cctgaaggaa gttgaaaaca actgcaacga tgccagcctg cgcgcagagc
     7501 gcgaggccaa ggtggtagaa gccgaagatg aagtgagcga gcgcgaaggc gatctacgcg
     7561 aagccctggc cgacggcgat caggacgaca tcagcaagcg ccaacacaaa cttgaagaag
     7621 cccgcgcaga actgcagcaa gcgcgcgaag agctaagcaa gtagctcaat taaaacgcgc
     7681 gaaactcttc atgacatttc ttgcagctat cctcgacacg ctgcatcggc gcggtcagcg
     7741 cctcgggcgt tagcggttgc gcggcggtgg cggctaccaa cgcagcggtg ctggcttcca
     7801 tggcctgggc caactcctgg aaacgagcct gacgctgcca gacgtcatcc ttggcgcggc
     7861 tctcctcacc ctcgtcacgc acctgcggga agtgctgcca aggcgtgcgg gtcagttgat
     7921 cgagtttggc cgcgccatcg ataaatgcct gttccttgaa cgccaaccgc ccgcgcagca
     7981 tgccgccgag gtcttcgctg accttgagca tttgcttgaa aatgctctga cgctggcctt
     8041 gcggcgaatt gggatcgaca ccgccgcagc cacttgcagc cagcgccagt acaaccaggg
     8101 acagggtttt aagcttcata acaccttcaa tgccaacaac cgggtggata acgggcgaca
     8161 gtatcgccac ccgccatcca gcggccaagc gtggtgtttt gccgcagatg tcagaacctg
     8221 cgtgagcgct ggggcgcaga cgttaagcgg agaccacaaa caggctgacg atcagcgcca
     8281 tcccggcgaa ccacaccagc gaacggatcg gcccccagtc ggccaggtag cacaggaaat
     8341 acagcacccg gctgagcacg aaggcaatcg ccagctgatc cagcagcccc tgctcggcgc
     8401 cgccagcctg gtgggcgatg atcaccgccg cagcgaatgc cggagtcacc tcgaagccgt
     8461 tgagctgggc attgttggcg cgcttgcgcc agccctccag ggtactcaga aacgcacgag
     8521 gatcctgatt ggcgcgcggt ccgtagccgg ggccaaggaa cttggcagcg gcggtactca
     8581 ggtaaggtag gacgatggcg atcagcacgc accagtaggc gagggtcatg gaagctccgc
     8641 agttcggtaa tggaatgcgc agcgtgtccc gagcaggaac gggcgtccat gactgccacc
     8701 acctaagtcg acggcgattc agccaatcat gccgccagtt tgcccttggc cagcgccgcg
     8761 ctggccgcca gcatagcgcg cagcaatacc gcacaaccgg cggcgagatc tgccggcgcg
     8821 gcattctcga tttcgttgtg gctaatgccg ccctcgcacg gcacgaagat catcccggcc
     8881 gggcccagtt cggcgagaaa gatcgcgtca tgcccggcac cgctgacgat atccatatgc
     8941 gacagcccca gcccagccgc cgcgccgcgc acggcctcga cgcagccctg atcgaaatac
     9001 agcggcggga aatctgcggt cggcatcaac tcgaaactca gcccgtgttt ggcgcaggtc
     9061 gcctcgatta ccccacgcac ctcggcaatc atcgaatcca ggcgtgccgg ctccaggtga
     9121 cggaagtcca gggtcatgcg tacctcgccg ggaatcacat tgcgcgagcc ggggtaagcc
     9181 tgcagacaac cgaccgtgcc acaggcatgc ggctgatgct ccagcgccac ccggttgacc
     9241 gccgcaacca cggccgaggc accgaccagg gcatccttgc gcaggtgcat cggcgttggc
     9301 ccggcatggg cctcgacgcc tttgagattg agatcgaacc acttctgccc gagtgcaccg
     9361 agcaccacac cgatggtctt gccctgatct tcgagaatcg gcccctgctc gatatgcgcc
     9421 tcgaaatagg cccccaccgc atggccgctc accgcgcggc taccggagta gccgatggcg
     9481 ttcaatgcct caccgacgct gacaccctcg gcgtcgcgct tggccagggt ttcctccagg
     9541 gtgaacttct cggcgaacac ccccgaaccc atcatgcacg gggcaaagcg cgagccttcc
     9601 tcgttggtcc acaccaccac ttccagcggc gcttcagtct gcacgcccag atcattgagg
     9661 gtgcgcagca cctcgacgcc ggccagcacg ccgaaacagc cgtcgaactt gccgccggtg
     9721 ggctgggtgt cgatatggct gccggtcatc accggcggca acgcggcgtt atgcccaggg
     9781 cgacgggcaa agatattgcc gacgccatcg accgtcaccg tgcagccggc ctcctcgcac
     9841 cagcgcacaa acaggtcgcg cgcctggcgg tcgaggtcgg tcagcgccaa gcggcaaacc
     9901 ccgcctttga cggtggcacc gagctgcgcc agctccatca gcgattgcca caggcgctcg
     9961 ctgttgatat gcggctggct ggattgcaga acctcgatgg cagtgttcat ggtgatctcc
    10021 ttgggccttt ctgattttcg ttagcgctgg gtgcgcctgc tcaatcgttt cgcgggcaag
    10081 cccgctccta caggtgcgcg ttgttcttgt aggagcggct gggcggcatt ccgctgggcg
    10141 gcgttccgat tgtccgcgat gttttgtctt aaaccggcgc cttggccgct acaggctgcg
    10201 cgctacgcag gctgcacaag ccgaaataga tcagcccgcc caggaaggag ccggtaaacc
    10261 agccgtagtc gtagaaccag ctgaaggcgc tgctgccgag cgacagcagg gtcagcgcga
    10321 cgggtacggc aaaggcgaca aagccgatcc agttccacgc cgggtagacg tcgtcgcggt
    10381 acaggccggc caggtcgagg cgctgtttct tgatcaggaa gtagtccacc accatgatcc
    10441 cggcaatcgg ccccagtagg ctggaataac ccagcaacca gttggaatac acgctttcca
    10501 ggctcaggtc ggagaccagc aggccaagct tttttagcag ctcgtggccc atcaggccga
    10561 gaccgatcag gccggtgagg atcaccgctt tggtgcggtt gatcagcttg ggcgcgatgt
    10621 tctgaaagtc gttggtgggc gagacgatat tcgctgcggt gttggtcgac agcgtggcga
    10681 cgataatcag tgccatggcc agggccaccc agccggggct ctggatatga ccgatcaggg
    10741 tcaccgggtc ggacacggtt tcacccacca gcgaggccga ggcggcggtc atcaccacgc
    10801 ccagggcggc gaacaggaac atggtcagcg gcaggccaat gacctggccg atgatctgat
    10861 ccttctggct cttggcgtag cggctgaagt cgggaatgtt cagtgacaac gtcgcccaga
    10921 aaccgaccat ggcggtcagg ccggcgagaa agtaactggt cacgctggcg ccttccgggc
    10981 gattggccgg ctgcgccagc agttcgctca tcgacacatt gggcagcgcc cacaccaaca
    11041 ggccggcacc gaccagcacc agcagcggcg cggagagggt ttccagccac ttgatcgact
    11101 cggcaccgcg cagcactacc cacaggttga gcacccagaa gatcataaaa ccaatgacct
    11161 cgccggtacc gcccaggctc ttccagccct cgaagatcga accgagcagc aggtggatgg
    11221 ccaggccgcc gaacatggtc tgaataccaa accagccgca agcgacgacg gcgcgaatca
    11281 ggcagggcac gttggagccg attaccccaa atgacgagcg cagcaacacc ggaaagggaa
    11341 tgccgtactt ggtgccgggg aaggcgttca gcgtcagcgg aatcagcacg atgatattgg
    11401 ccagcagaat cgccagcagc gcctcgccca ccgacaagcc aaagtaggcg gtgagtacgc
    11461 cgcccaaggt gtaggtcggc acgcagatcg acatgccgac ccacagcgcg gtgatatgcc
    11521 acttgttcca agtgcgctca tgcaccttgg tcggggcgat gtcgtggtta tagcgcgggc
    11581 tgtcgagtac gtcggggcca gcggccagtt cgtacaagcc atcgcgttca gtcacttgcg
    11641 atctgctctg ttgcatgggc ctctccagtc attgttctga ttattcgctc atcgggctta
    11701 accgccgtcg ggcagtggtt gcactgccaa acggccgttc gcgatggccg gagtacattt
    11761 ccatctcagg gccggccagc ttggtagcga cggctatcaa taagcgtgcc gggaacctgt
    11821 tcaactcatc tatcacgcac agtaaaaaca gctaatcaat tgttttataa ggatttcaat
    11881 aaacaaagcc ggtttgcatt acctgctttg ccgggccgtt ccagacccag cacgcaagcc
    11941 tgtctgccta gtcaggattc aaagtggtgc aggcaaatat tttttctgcc gccaccgcct
    12001 attcgccgcg tggttcaagc gcctgatttt gcataggaaa tcttgaccat atttggttct
    12061 tgtcaagtgc gtcaaaatgg tgagcggctg caccattttg cagactttta tatttttacc
    12121 tttaatatca acaacttaaa tacacaaaaa ggcacttaaa aatattcttg atcaatcaca
    12181 tagcagcgac tagattcaat tcctgtcagg catgacagga atacaagccc ctcgcctgac
    12241 aggccataac accaagaacc ggcacgcacc ggtcagcctg agaggaactc gctatgtcgc
    12301 tgttgatccg tggtgccacc ctggtcaccc atgaggaaag ctaccgcgcc gatgtgctct
    12361 gcgccgatgg tctgatccag gccattggcg acaaccttga tgcccccgcg ggctgtcagg
    12421 tactcgacgg cagcggccag tacctgatgc ccggcggcat cgatccgcac actcatatgc
    12481 aactgccgtt catgggcacc gtggccagcg aagacttctt cagcggcacg gctgcagggc
    12541 tagccggcgg caccacctcg atcatcgatt tcgtgattcc caacccgcag caatcattgc
    12601 tcgaagcctt tcacacctgg cgcggctggg cggaaaagtc cgcgtccgac tacggatttc
    12661 atgtcgccat cacctggtgg agcgacgagg tgagccgcga aatgggcgag ctggtggcca
    12721 agttcggggt caacagcttc aagcacttta tggcctacaa gaacgccatc atggccgccg
    12781 acgacaccct ggtggcgagc ttcgagcgct gcctggagct gggcgcggtg cccaccgtgc
    12841 acgcggaaaa tggcgagctg gtctatcacc tgcaacaaaa gctcctggcc cagggcctca
    12901 ccgggccaga agcgcacccg ctgtcgcgcc cctcgcaggt tgaaggcgaa gccgccagtc
    12961 gcgccattcg catcgccgaa accctcggta cgccgctgta cctggtacac gtctcgacca
    13021 aagaagccct ggatgaaatc acctacgccc gcgccaaggg ccagccggtg tacggcgagg
    13081 tgctggccgg ccatctgctg ctggacgaca gcgtgtaccg ccaccccgat tggcagaccg
    13141 ccgccggtta tgtgatgagc ccgccgttcc gcccgctcgg ccatcaggag gcgctgtggc
    13201 acggcctgca atcgggcaac ctgcacacca ccgccaccga ccattgctgc ttctgcgccg
    13261 agcaaaaagc cgccggccgc gatgatttca gcaaaattcc caacggcacg gccggcattg
    13321 aagaccgtat ggcggtgctg tgggatgaag gggtcaacag cggcaagctg tcgatcaatg
    13381 acttcgtcgc gctgaccagc accaacaccg cgaaaatctt caacctgttc ccacgcaaag
    13441 gcgcgctgcg cgtcggcgcc gatgccgacc tggtgctctg ggacccacag ggcacgcgga
    13501 ccatctcggc caagacgcat caccagcagg tcgatttcaa tatcttcgaa ggcaagaccg
    13561 tgcgcggcgt gcccagccac accatcagcc agggcaagct ggtctgggtc gatggcgacc
    13621 tgcgcgccga acgcggtgct ggccgctatg tcgagcgacc agcctacccg gcggtgttcg
    13681 atctgctgag caaacgcgct gaacagaatc gtccgaccgc cgtcgagcgc tgagacagaa
    13741 cctgggtccg tgggaggggc tttagccgcg acatatccag ggatatcgac attctcgcgg
    13801 ctaaagcccc tcccacttaa gagttccacc accgatgcaa cacctatacg ccacaccagc
    13861 ccgcagaggc tggacggccg cttatacgct gttaactgcc caataaaagc cacaaaaccg
    13921 tgaggcaata accgtgatag acaccctcag ccatttgccg cgaccggatg ccggcgccgc
    13981 cgaactggcc gatcgtttca gcgatctggc cccgccgctc accgctcgcc aggccgccct
    14041 ggaaagttcg cgctgcctgt attgctacga cgcgccgtgc gttaacgcct gccccagcga
    14101 gatcgacatc ccctcgttta ttcgcaatat cagccatgaa aacgttcaag gcgccgccga
    14161 gaaaattctc tcggcgaata ttcttggcgg cagctgcgcc cgcgtctgcc ctaccgaaat
    14221 tctttgccag caagcctgcg tgcgtaacaa cgcccaggaa tgcgcgccgg tgctgatcgg
    14281 cctgttgcag cgctacgcca tcgacaacgc gcagttcact gagcacccgt tccagcgcgc
    14341 ccctgccagc ggtaagcgta tcgccatggt cggcgcggga ccggcgggtt tgtcctgcgc
    14401 ccatcgcctg gccatgcacg gccatgaggt ggtgatcttc gaagcccgcg agaaagccgg
    14461 cggccttaat gaatacggca tcgccgccta caagctggtg gatgacttcg cccagcgcga
    14521 gatggagttc ctcctgcaga tcggcggcat cgagattcgt cacggccaca aactgggcga
    14581 cgacctcagc ctgagcgagc tgcaccagca gttcgacgcg gtgttcctcg gcatcggtct
    14641 agcggccagc aagcgcctcg gcctgccggg cgaagacgcg ccgggcctgc tcgccgccac
    14701 cgattacatc cgcgaactgc gccagagcga tgacctgacc caactgcctg tggccgagcg
    14761 ctgcatcgtg ctcggtgccg gcaataccgc catcgatatg gccgtgcaga tgagcaaact
    14821 cggcgcgcgc gacgtcaacc tggtgtaccg ccgtggcttc gaggaaatgg gcgcaaccga
    14881 acacgaacag gacatcgcca aagccaatca ggtacgcctg ctgacctggg cgcaaccgga
    14941 cgccgtgctg ctcaacgagc agggccaggt gcgtggcatg cgcttcgccc gtacccgcgt
    15001 ggagaacggc cgcctgcaga gcaccgggca gaccttcgag ctcgccgccg acgccatctt
    15061 taccgccatc ggccaggcct ttgatggttc agccctgagc gacccgctgg cccgcgagct
    15121 gaagcgtgag ggcgaacgca tttgggtcga cgcacagctg caaaccagcg tgcccggcgt
    15181 gtacgccggc ggcgactgca ccgccctcgg ccaggacctc accgtacaag ccgtgcagca
    15241 cggtaagctg gccgccgagg ccatccactc tcatctgatg ctcaatgtgg aggccgcata
    15301 aatggccgat ctatccatcg aattcgccgg tatcaaggca cccaatccgt tctggctggc
    15361 cagcgcaccg cccaccgaca aggcctacaa cgtggtccgc gccttcgaag ccggctgggg
    15421 cggcgtggtg tggaagaccc tcggcgaaga cccggcggcg gtcaacgtat cgtcgcgcta
    15481 ttcggcgcat tttggctcga accgccaagt gcagggcatc aacaatatcg aactgatcac
    15541 cgaccgctcg ctggagatca acctgcgcga gatcacccag gtaaagaagg actggccgga
    15601 ccgcgcgctg atcgtctcgc tgatggtgcc ctgcgtcgaa gaatcctgga aagccatcct
    15661 gccgctggtg gaagccaccg gttgcgacgg catcgaactg aacttcggct gcccccacgg
    15721 catgcctgag cgcggcatgg gcgcagcggt cggccaagtg ccggaatatg tggagatggt
    15781 cacccgctgg tgcaagacct acagctcgct gccggtgatc gtcaagctga cgccgaatat
    15841 caccgacgtg cgcctgtccg cccgcgccgc ccatcgcggc ggggccgatg cggtgtcgct
    15901 gatcaacacc atcaactcga tcaccagcgt cgacctggag cgcatggtcg cccaccccat
    15961 agtcgggacg cagagcaccc atggcggcta ctgcggttcg gcggtcaaac ccatcgccct
    16021 gaatatggtc gccgagatcg cccgcgaccc gaaaaccctt ggcctgccga tctgcggtat
    16081 cggcggcatc ggcagctggc gcgatgcggc ggagttcgta gcactgggct gcggcgcggt
    16141 acaggtgtgc acggccgcca tgctgcatgg ctttcgcatc gtcgaggaaa tgaaggatgg
    16201 cctgtcgcgc tggatggaca gtcagggcta ccagagcctt gccgatttct ccggccgcgc
    16261 agtgggcaac accaccgact ggaagtacct ggatatcaac taccaggtga tcgccaagat
    16321 cgatcaggac gcctgcatcg gctgcggccg ctgccatatc gcctgcgaag acacctcgca
    16381 ccaggccatc gccagcctga cccagaccga cggcacgcat aagtacgaag tgatcgacga
    16441 ggagtgcgtg ggctgcaacc tgtgccagat cacctgcccg gtggagaact gcatcgagat
    16501 ggtgccccag gacaccggca aacccttcct cgactggaac cacgacccgc gcaatccgta
    16561 tcgtgtagcg tcctagcccg gcaattcgcc ggaacctatc gcgggcaagg actaggcgtc
    16621 cccaccgctc ctacaacaac tctgcacctg taggagcggg catgcccgcg atcaggcttt
    16681 cagggctcca acccaatacc acgcaatatc acactggtca ccgtctgcac cgcgcgctcg
    16741 aactgcagtt cggacagcgc ctggtgatca ttgagaatcg ccacctgatg gctgaaatcg
    16801 gcgtagtgct gagtcgaggc ccagatcata tacagcaagg cggatggctc gacggcgagg
    16861 atacgtttgt cctcgatcca ctggcggatc ttcgcctcct tcatcttggc ccagctgtag
    16921 aggctgtcgt cgaggcttgc gccgagcatc ggcgcaccat ggatgatctc gtcggcccag
    16981 accttggagc cgtgcggacg gctgcgcgaa tggttcatct tggcgcgg
//
LOCUS       DASRUM010000010        27950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2178554, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000010 DASRUM010000000
VERSION     DASRUM010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 27950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27950
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2178554"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VFF22_00610"
     CDS             complement(<1..742)
                     /locus_tag="VFF22_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZX15358.1"
                     /translation="MTLRLSGASLSHGNGVHALRGVDLHIAAGERVAIIGPSGAGKSS
                     LLNLLATALQPSDGEIQVLGEQPWHLSSRQRQRLRARIGLVHQAPPLPARQRVVTAVL
                     AGKLGQWSLGKSLLNLLHPLDIPGARRELAKLDLADKLFAQCQQLSGGQLQRVGIARV
                     LYQAPGLLLADEPVSAMDPVLAEHTLNVLCRHAETHGVTLVASLHAVELALAHFPRII
                     GLRDGQILFDRPASEVDQAQLDALYANEQ"
     gene            complement(739..1590)
                     /locus_tag="VFF22_00615"
     CDS             complement(739..1590)
                     /locus_tag="VFF22_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate ABC transporter
                     substrate-binding protein"
                     /protein_id="HZX15359.1"
                     /translation="MLKRTLALAAGFALCLSANLSQAAETLRVSAIPDEAPTELLRKF
                     KPLGAYLEQQLGMKVEFTPVADYPAVVEALATDRLDLAWLGGFTFVQVRLKTGNAIPL
                     VQREQDAQFTSKFITANPDVTSLADLKGKTFAFGSVSSTSGSLMPRYFMLQDGIKPES
                     HFSRVGYSGAHDATAAWVQAGKVDGGVLNASVWDKLVASGKVDSNKVKVFATTPTYFD
                     YNWTVRGTLDPALAEKIKAAFLALDPANPEHKAILDLQAASRFIETSPENYKGIEDAA
                     RAADLLK"
     gene            1844..2878
                     /gene="selD"
                     /locus_tag="VFF22_00620"
     CDS             1844..2878
                     /gene="selD"
                     /locus_tag="VFF22_00620"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HZX15360.1"
                     /translation="MSEPIRLTQYSHGAGCGCKISPKVLEVILAGSGAQNLDPKLWVG
                     NASRDDAAVYALDDERGVVSTTDFFMPIVDDPYDFGRIAATNAISDIYAMGGDPLMAI
                     AILGWPVNLLPPEVAREVIRGGRAVCDEAGIPLAGGHSIDAPEPIFGLAVTGVVLKRH
                     MKRNDTATAGCQLYLSKPLGIGILTTAEKKAKLRPEDVGLARDWMCTLNKPGSRFGKL
                     AGVTAMTDVTGFGLLGHLVEMADGANLTAQLDYDAVPRLPGVDYYLAEGCVPGGTLRN
                     FDSYGEKIAPITQEQQNLLCDPQTSGGLLVAVTPEGEAEFLAVAAELGLNLAPIGRLV
                     ERQRYAVEVL"
     gene            2878..3981
                     /gene="mnmH"
                     /locus_tag="VFF22_00625"
     CDS             2878..3981
                     /gene="mnmH"
                     /locus_tag="VFF22_00625"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003381312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HZX15361.1"
                     /translation="MRDNSNNYRDIFLNDLPMMDARAPVEFSKGAFPGVLNLPLMDDS
                     ERQRVGTCYKQHGQDAAIALGHQLVSGQVKAERVAAWAAFAKANPHGYLYCFRGGLRS
                     QITQQWLAEAGIDYPRVIGGYKAMRTFLLETTQQAIAECDFVILGGLTGTGKTDVLVQ
                     LNNSLDLEGHANHRGSSFGKRASGQPAQIDFENRLAIDLLKQRARGIEQFVLEDEGRM
                     VGSCTVPLELYQGMQRYPLVWLEDAFEDRVERILRDYVVNLCAEFIALHGEEEGFRLY
                     AERLLQSLSNIQKRLGGERYQRLLTIMQAALDEQRSGAVALHRDWIAGLLREYYDPMY
                     AYQRDSKAARIEFAGDQAAVIAYLTERKANRQG"
     gene            complement(4010..4414)
                     /locus_tag="VFF22_00630"
     CDS             complement(4010..4414)
                     /locus_tag="VFF22_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZX15362.1"
                     /translation="MNQRDVVFPPGRHALYERNRYSPAVRSNGFLFVSGQVGSREDGS
                     PEPELEEQVRTAFNNLNAILHAAGGSFDDVVDVTVFMVDPQATFERIWKVVPEFWGNA
                     PYPTITAVGVTWLYGFQFEIKVIAKLPEATSS"
     gene            4559..5506
                     /locus_tag="VFF22_00635"
     CDS             4559..5506
                     /locus_tag="VFF22_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZX15363.1"
                     /translation="MDRFAAMQAFARVVEAGSFTKAAETLHMSKTSVSQLVQQLEARL
                     RVKLLNRTTRKVNLTADGALYYERVLRLLDDLADAETSLSGASAVPRGRLRVDVPSPL
                     ARMILMPALPAFHARYPEIQIDMGVSDRIVDLIGEKVDCVVRGGELTDQSLMARRVGD
                     LQLGVYAAPSYLQRLGTPAHPRELEDSQHRIVGFLWARTGKALPYAMHLAGESIHIQG
                     RYVLAVDDGNAYLAAGLAGMGVLWLPDYMSAPHVAQGELLPLFTDWRLEPMPLYVAFP
                     PNRHVSIKLRVFIEWVAELMAEHAPISQRVLPRASFPKH"
     gene            5691..6053
                     /locus_tag="VFF22_00640"
     CDS             5691..6053
                     /locus_tag="VFF22_00640"
                     /inference="COORDINATES: protein motif:HMM:NF017742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HZX15364.1"
                     /translation="MQNPTFNPASNGQSAPSKPFSSVSASSVSREFQNFLADLEDLVK
                     ETTSLTGEELAQAKEKLNTRLATAKASLEHMNESVVQRAKQSAAVTNEYVHEQPWKAL
                     GASTVIAFLLGLAVARRN"
     gene            6065..6493
                     /locus_tag="VFF22_00645"
     CDS             6065..6493
                     /locus_tag="VFF22_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZX15365.1"
                     /translation="MNQNPEEQPSAATHAAEGLSLFALLRLLRSAGRAVFAQAALHGQ
                     LARVEWAEEKQRLLRILVTTVFGFVCLLCLMLLGNALVLALSWATPYRIHALVGLICL
                     HGLAVAIAWHHVRRLVRQGSSFAATRAEVAADIALIKRNL"
     gene            6490..6792
                     /locus_tag="VFF22_00650"
     CDS             6490..6792
                     /locus_tag="VFF22_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15366.1"
                     /translation="MSTLPASALEEQRQALRRRLLQQRQRIGYLLEPPVGGDNQFPRS
                     MTMRLLTRRPAVTMRLASQMALLLCGPRIIRTVSGAMLLVRLLRAVSAEQRRPPPR"
     gene            complement(6795..7502)
                     /locus_tag="VFF22_00655"
     CDS             complement(6795..7502)
                     /locus_tag="VFF22_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX15367.1"
                     /translation="MHKPHLLLLPGMLSDARLWQHQAAALADLAYVTVADLSQADSIA
                     EMASKVLDQAPDENFALAGLSLGGYVALEIMRQAPQRVLGLALLDTSAHADSDETKQG
                     RQALMAKAEHEFPAVIAGLLPKLLQPEHQADENLTEVVTAMANSLGQGAFMRQQRAML
                     GRIDSRPSLAQISCPTLVLCGREDAITPLDVHEELAAAIDGAQLVVLDDCGHLSSLEQ
                     PERVAQELERWLQRIQA"
     gene            complement(7651..8943)
                     /gene="cls"
                     /locus_tag="VFF22_00660"
     CDS             complement(7651..8943)
                     /gene="cls"
                     /locus_tag="VFF22_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HZX15368.1"
                     /translation="MAHNRAPVALDDANGPLSTARGQAIIERLQARDASSTFFDLHLA
                     IEESIAGSPLTTGNRVELLQDGPRTYQSMLEAIAGARDHINMESYIFDGDEIGQRFAD
                     ALIAKQQAGVQVNLIHDSVGTLGTATSFFSRLSETGVQVVEFNPINPLTAKAGWTLNQ
                     RDHRKLLIVDGRIAFLGGINISSVYSGSSLRLGNQAEPAADVPWRDTHLRLEGPVVAE
                     LQQLFIETWAKQKGPPLNTAQYFPVAEARGNEVVRAIGSSPDDPYSLIYVTLISALRS
                     AQREIWLTNAYFVPDPQLLATLKEAVARGVDVRLILPSRTDSWLVFHAGRAYYDELLR
                     AGVRIYERHEALLHVKTAVIDGVWSTVGSTNLDWRSFLHNQEVNAVVLGNDFGDKMRA
                     AFRADLEKSREITLPDWQARPLDMRAKELFGRLWEYWL"
     gene            complement(9195..11489)
                     /locus_tag="VFF22_00665"
     CDS             complement(9195..11489)
                     /locus_tag="VFF22_00665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HZX15369.1"
                     /translation="MNYQVAVRALCEFTAKVGDLDLRFTPSPTAQEGMTGHAIVVARR
                     GPGYYAELPLSGVYQGLQVRGRADGYNPDENLLEEIKTHRGDISRIPENHRLLHWAQV
                     KIYGWLLCQERDMAEINLAVVYFNVITQHETAFHLTVSADELREFFELQCSRYIRWAE
                     YEREHRRQRDEALLRLRFPHAEFRSGQRQLANQVYRAARDGHCLMAQATTGIGKTLGT
                     LFPQLKAFPEQQLDRLFFLTAKTPGRRLALDALATLRSQEQAMPLRVLEHVARDKACE
                     HPDKACHGDSCPLAKGFYDRLPAARQAALEGQWLSHESVRATALAHQICPYYLSQELC
                     RWADVVVCDYNYYFDMGGLLYGLTLLNEWRITLLVDEAHNLIERARGMYTAELNQQRF
                     EALRRIAPNVMKAPLERISRHWRQLERDQEVAYQVYPTAPDLFVMALQKAVSTLTDHL
                     SEQPAGNAGELLHFYLDAMLFCRLAEAFGAHSLFDISRDESSGQAVSTLCIRNIVPAP
                     FLAPRFDEAHSCTLFSATLSPANYYADLLGLPEETPWIDVESPFSAEQLQVQAVSNLS
                     TRFAHRDFSLAPIAALIARQYAEKPGNYLAFFSSYTYLQAALQELQMAHPQIPVWQQS
                     RQMDENERQAFLDRFASKGRGIGFAVLGGAFGEGIDLPGDRLIGAFIATLGMPQINPA
                     NEEIKQRMELMFGSNNGYGYTYLYPGLQKVVQAAGRVIRTVQDQGVVYLIDDRFNQPA
                     IRKLLPTWWKVDRCRLPLNQDTVV"
     gene            complement(11486..13150)
                     /locus_tag="VFF22_00670"
     CDS             complement(11486..13150)
                     /locus_tag="VFF22_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010678626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HZX15370.1"
                     /translation="MHTATDTPMQSQLDPHFYYLANFRQALTWLEERYADLLNEAEHE
                     FVRQFFAMDTPAQALLVRMIMRKGSHFRLSKLSYAEIGCARQAAAPLLDAGWVRHDAK
                     LSLEELFKLLRKDEVLQHLAGPTSRKSQNKSALLAQLAEACAEPRAFAAWCPGSEEQI
                     VSLTIDALCERLRLMFFGNLHQDWSEFVLAELGIFRYERVALTADSRAFRHRADVDCY
                     LHLQRSREQFEAGEPLANVLQQINAVACDTPFLAARRNKLLFRMGQQLEREADLEGAL
                     AFYSDCRYVGARLRQIRVLERLGRPEQAYALTLQAIQAPENDAEAQAVERALTRLTRQ
                     LGQPQPGKAKPVPPARIDLSLPRPEAQSVEFAVKAHLHEPDAPVHYVENSLICSLFGL
                     LCWPAIFAPVPGAFFHPFHTAPVDLFSADFHTQREELFASCLAHLDSDTYKQVIRENY
                     AAKFGIQSPFVFWAALDESLLEQALDCLPAAHLRAWFKRLLVDIKANRAGMPDLIQFW
                     PAEQRYRMIEVKGPGDRLQDNQRRWLAFCAEHGMPVDVCYVQWADA"
     gene            complement(13615..13884)
                     /locus_tag="VFF22_00675"
                     /pseudo
     CDS             complement(13615..13884)
                     /locus_tag="VFF22_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
     gene            14360..15244
                     /locus_tag="VFF22_00680"
     CDS             14360..15244
                     /locus_tag="VFF22_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019361095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HZX15371.1"
                     /translation="MTEKTVAPAEAPQSQEPRQYPLFGNDADSAPASSASDPIDKSFS
                     RYVVYVDESGDHGLENVDPNYPVFVLAFCVFHKNHYAQRVVPAIEAFKFRHFGHDLVV
                     LHETDIRKEKGGFRFNSRQHRELFLSELTDIIETSNFVLISCIIDKQRLRERTSNPYH
                     LALSFCLETLYELLEEKQQHQPTTHVVVECRGKREDAELELEFRRVCAGDNKFGKSLP
                     FEIVFADKKTNSSGLQLADLVARPIGLSVVKPQQPNRAFDLLKRKFFCSGGRMQVGIG
                     FDGWGLKIHPALESERPR"
     gene            15503..15715
                     /locus_tag="VFF22_00685"
     CDS             15503..15715
                     /locus_tag="VFF22_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15372.1"
                     /translation="MSNFQFLTSEFKNLFEPAKGAEQLMYSDPRACCMRTCHALEQLV
                     HWLYEHDRDANAIVAVIAAIKRSAAA"
     gene            15715..16719
                     /locus_tag="VFF22_00690"
     CDS             15715..16719
                     /locus_tag="VFF22_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009618448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HZX15373.1"
                     /translation="MAYQPPFTLNGSILSLVAEIAQSVGRLSAQPEHANALRLRRINR
                     IRTVQGSLAIEGNTLSEEQITAILDGKRIIAPPKDVQEARNALAVYEQLEAWQPANEQ
                     HLLNAHALLMKGLIDDAGHYRQGGVGVMKGDQVVHMAPPANRVSKLMRDLLQWLETTD
                     QPPLIASCVFHYEFEFIHPFADGNGRMGRLWQTLILSRWQPLFAHVPVESLVHAHQAE
                     YYAAINDSTQQADSAPFIGFMLSMLRDALLSMTPQVTPQVPPQVAELLRHMVGEVRRE
                     TLQAALGLADRKSFRERYLRPALDAGLIAMTRPDKPNSRLQCYYLTTLGKQIRGSAAL
                     "
     gene            complement(16878..17102)
                     /locus_tag="VFF22_00695"
     CDS             complement(16878..17102)
                     /locus_tag="VFF22_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HZX15374.1"
                     /translation="MPDSAMYFAIAVAALFALVNLWAVISVFRSDKTVGTKTLWALLI
                     WIVPIIGLVIWGVAGPRGHGPGPSSPEHSK"
     gene            complement(17339..17956)
                     /locus_tag="VFF22_00700"
     CDS             complement(17339..17956)
                     /locus_tag="VFF22_00700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014718614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZX15375.1"
                     /translation="MIGVVVPAHNEEALLGRCLQSLHNAARHSDLQGEPVCIVVVLDA
                     CSDASAQIARQHQVISLSIAAHNVGRARRAGAAHAISLGARWLACTDADSCVAADWLV
                     QQLQHDAEVVCGTVHLDDWEGYDDAVQRAYHQRYQPGDSHRHIHGANLGICVEAYKRA
                     GGFPPLRAHEDVRLIESLNEVGARIVWSGSNSVATSARHDSRARG"
     gene            complement(17953..18552)
                     /locus_tag="VFF22_00705"
     CDS             complement(17953..18552)
                     /locus_tag="VFF22_00705"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016391722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX15376.1"
                     /translation="MSLSADYFDALYAANDDPWHFRSRWYELRKRQLTLASLPRQRYQ
                     RAFELGCANGELSAELAQRCEHLLCCDASQRAVALARERLAGYAHAQVYAGQLPQCWP
                     EQCFDLIVISEVGYYLDQQALTNLLQHCIASLTPSGHLLACHWRHPIEGSPLDGEQVH
                     RLLRQILPFERIVLHQEPDFVLEAWSVSAFAINLHEVAR"
     gene            complement(18549..19313)
                     /locus_tag="VFF22_00710"
     CDS             complement(18549..19313)
                     /locus_tag="VFF22_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007249350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HZX15377.1"
                     /translation="MSEDDNLIQGQGTSAEAWASSQRLAALPVVALDELIAPGSRLAI
                     VAPHPDDEVLGCGGLLAQLAARDAPIRIVAVTDGEGSHPNSTLWPVATLRAIRQQESR
                     IALQCLGISAQRIEWTRLHLPDSGVALDEPRLRAHLEQALQGCSHVITTWRADGHCDH
                     EVVGRVSTAVAQATGATLYEMPVWAWHWATPEDPRIPWERACKLRLGEQTLARKRFAL
                     HAHRSQLLADPSTGAPPILTERTLARFAQPYEVYFR"
     gene            complement(19310..20350)
                     /locus_tag="VFF22_00715"
     CDS             complement(19310..20350)
                     /locus_tag="VFF22_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011333962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZX15378.1"
                     /translation="MTGKQSALVAAEQPMQLASALEAFSRRACEPANDAVPRQMRALM
                     AAGLDQLPTPGQGETLRRWQLLAEVAAADLSLLKLFEAHTDAQAILRELGASDENTEL
                     WAVWAAEPPQARVEITARNADGVTLHGCKAWCSGALHVDQALVTVWDEQGRSQLVALA
                     MNQPGVTVTRDGWQAVGMAAAASFEIRLEGASARCIGEPGAYTARPGFWHGGAGIAAC
                     WYGAGVAIARHVQQHMRSDPHALAHLGALDTALSGAKQALRACASWIDKQPTAAAEYP
                     ARLVRAQVEAALEQVMCHAGRALGAGPFCRDPHFARLLADLPVFLRQSHAERDLERLG
                     ELLLHSDKDWNL"
     gene            complement(20347..22875)
                     /gene="ligD"
                     /locus_tag="VFF22_00720"
     CDS             complement(20347..22875)
                     /gene="ligD"
                     /locus_tag="VFF22_00720"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HZX15379.1"
                     /translation="MAKAQSDYNRKRNFEQTSEPREQPRRRGSKAGALRFVIQKHDAR
                     HLHYDFRLELDGTLKSWAVPKGPSLDPRDKRLAVHVEDHPLDYASFEGSIPEGNYGAG
                     DVIVWDQGIWQPHDDPVGAYAAGKLKFSLIGEKLSGDWALVRTRLRGSGDKEQWLLIK
                     ERDDTARSSDEYDIVNEQPQSVISGALVGGPSKTAKGKKAASTRKPAAGRSDFPASLA
                     PQLATLSDRPPEGDWTYEVKFDGYRILTRISDGEVRLFSRNGHDWSERLPLQAKALAK
                     LGLRDSWLDGEVVALNDIGLPDFQQLQNAFDIGRSHDLVYYLFDAPFINGEDLRQTPL
                     EQRRALLKQLLPSTRKGPLRYSADFNAHHQDILDSACAMSLEGVIGKRVGSAYVSRRS
                     ADWIKLKCRLRQEFVIVGFTQPKGSRSGFGALLLAVNDDAGLRYAGRVGTGFDETALK
                     ALHQRLQQLAQADSPLAQKLTSAQAREVNWIKPELVGEVEFAEWTREGIVRQASFIAL
                     RSDKPASQIIREHPQWPDATKPEKNAEPASAAGKPLLAGVAISSAQRLIDPQSGTSKL
                     QLAQFYESVADWLLPHLAGRPVSLVRAPEGVSGEQFFQKHAEHLAIPQIKHLDPKLDP
                     GHAALMQIDSLPALIGATQMGCIELHTWGATSTRIDTPDRFVLDLDPDPALPWRSMLE
                     ATRLTLGVLDELNLQAFLKTSGGKGMHIVVPLARRADWDTVKAFAKAIAQFMTKQLPE
                     RFTATSGPKNRVGKIFIDYLRNARGASTVAAYSVRARPGLPVSVPISRDELDQVRSAQ
                     QWTVANLRQRLEQLARDPWHGYANQQRLNRGMWDKLGVIHRSTR"
     gene            complement(22891..23748)
                     /locus_tag="VFF22_00725"
     CDS             complement(22891..23748)
                     /locus_tag="VFF22_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017338703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HZX15380.1"
                     /translation="MPRAIWKGAISFGLVHIPVALTSATASQGVDFDWLDKRSMEPVG
                     YKRVNKVTGKEVSKENIVKGVAYEKGRYVVLSAEEIRAAHPEATQTIDIFAFVDSEKI
                     PLQHIDTPYYLAPDRRGEKVYALLREALSSTNKVALAHVVLHTKQHLAALMPLESALV
                     LVMLRWPSEVRSLASLELGAAVSKPKLAKSELDMAKRLIKDMSGDWQPDAYVDEFQNR
                     IMELVARKAKAGKIEEVDRVEGEDTRRSADVIDLTELLKRSLGGKPARKAPGKSTGSH
                     KPAARTKRN"
     gene            complement(23741..23893)
                     /locus_tag="VFF22_00730"
     CDS             complement(23741..23893)
                     /locus_tag="VFF22_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_099481817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSPA7_2676 family Cys-rich small protein"
                     /protein_id="HZX15381.1"
                     /translation="MTIRCLIAGCVWSSGTAIRMGRETLLCQRCSRCGSFHYVPESLA
                     GDGQHA"
     gene            24265..24543
                     /locus_tag="VFF22_00735"
     CDS             24265..24543
                     /locus_tag="VFF22_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15382.1"
                     /translation="MKGLSLCSIVAIAMLASPAFATDICTVNLQKLSDSKAALTTMTS
                     PLKEQVEERIKAAEEAQRAGDLETCGTHAEKALMLLQRPSKDRSTDAQ"
     gene            24659..24904
                     /locus_tag="VFF22_00740"
     CDS             24659..24904
                     /locus_tag="VFF22_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15383.1"
                     /translation="MYRLAAFLTAALLSTSLWAMHCPADMAKIDGLLKTDPPSDATVL
                     AQVQQLRAEGEALHKAGNHGESVKVLGEALQLLEASE"
     gene            complement(24942..27860)
                     /locus_tag="VFF22_00745"
     CDS             complement(24942..27860)
                     /locus_tag="VFF22_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX15384.1"
                     /translation="MPRASRYPLRQWIWRAFVQSALIPLILVESVLITVYLLSNAAIR
                     DAQIDHLQQSALDDLSAAVAREGQVIDSRLRSVEAQIQIFRDAAANALANTDFQPDAL
                     ERQRHAITDNGVFYSRSDDGRAASFYANSTPLAQQDHAKALRLSQLDPLMRSIQAANP
                     RVAAAYFNTWDSYNRIYPFFMTPEQYPHDMVIPDYNFYYLADAKHNPERKVAWTDVYL
                     DPAGMGWMMSAVAPVYRGDFLEGVVGLDITVGQMLDEIGELDVPWQGYAMLVSRDHNI
                     MALPSAGEEDFGLRELTEYSYAEAVRREVLKPEDFSLAKRAEMQPLLQAMTTGQGNVQ
                     EVVLGGRNQLIAWSEIPQTGWQLLLVVDEEHIFSETNRLAERYLHIGYLLIVGLALFY
                     LLFFALMWARSRRLSEQLVQPIDGIVGMMRELGQGNYQPAAPTSQIDELVSMAAAVQH
                     TGEQLQASETQRVEAQRILQLVLESTTESLWEVEAETMSITLSERFLRRFGLTHSNMS
                     FSEFNQRVHPNDLERIRHLRQLFANSGEDRFEAEYRYADALGEYVWLLSRGKVLERDS
                     EGRALRVAGTHVDITRLKQVQEDLRRASLDAQAASQAKSRFLSSMSHELRTPLNAIYG
                     FGQLIEMEAQDKPEAKLEADYAREIVNASRHLTSLVDDILDLSSIESRRQQLKLQPVE
                     VGALLHSCAELVQPEVQQRQLQLQVLAEADAGLYVQADMRRVRQVVLNLLSNAIKYNS
                     PQGLIRMGYEVRSNCVRLWVEDNGTGLTTEQQANLFQPFQRLGRENSSIPGTGIGLVL
                     CRELATLMAGEMGFSSSAGTGSRFWIDLPGAAAPAAQTAADVESVAAQPKQLAEVLCV
                     EDHPACLKVVQEALREFAEVRGAGSVQRALAELEHCTPTLLLLDLDLPDGDGMQVLDA
                     MRLNPRLQAVPVLVISAAADEAVFAEARRRGAQACLAKPIDLAQVRRVALSLLGQALK
                     "
BASE COUNT         5241 a         8628 c         8767 g         5314 t
ORIGIN      
        1 gttgctcatt ggcatacagc gcgtcgagct gcgcctggtc gacttcgctg gcggggcggt
       61 cgaacaggat ctgcccgtca cgcaggccga taatccgcgg gaagtgcgcc agggccagtt
      121 ccaccgcatg caggctggcg accagggtca cgccatgggt ttcggcatgc cgacacaata
      181 cgttgagcgt gtgctcggcc agcaccgggt ccatggctga caccggctca tcggccagca
      241 gcagtcccgg tgcctggtac agcacgcggg cgatgccaac gcgctgcagc tgaccaccgg
      301 aaagctgctg acactgggcg aacagcttgt cggccaggtc cagcttggcc agctcacggc
      361 gagcgccggg gatatccagc ggatgcagca gattcagcag gcttttgccc aagctccact
      421 ggccgagctt gccggccagc acggcagtaa ccacgcgctg gcgcgccggc aggggtggcg
      481 cctgatgcac cagaccaatg cgcgcacgca ggcgttggcg ctggcggctg gacaggtgcc
      541 acggctgctc accgagcacc tgaatctcgc cgtcactcgg ttgcagggca gtggccagca
      601 ggttgagtag gctcgacttg ccggccccgg acgggccgat gatggcgacg cgctcaccgg
      661 cggcgatatg taagtccacg ccgcgcaggg cgtggactcc gttaccgtgg ctgaggctgg
      721 cgccggagag gcgcagagtc atttcagcag gtcagcggcg cgcgcagcgt cctcgatgcc
      781 cttgtagttc tcaggcgagg tttcgataaa gcggctggcg gcctgcaggt caaggatcgc
      841 cttgtgctcc gggttggccg gatcgagggc gaggaaggcg gctttgatct tctcggccag
      901 ggctgggtcg agggtgccac gcacggtcca gttgtagtcg aagtaagtcg gcgtggtggc
      961 gaacaccttg accttgttgc tatcaacctt gccgctggca accagcttgt cccacacgct
     1021 ggcgttgagc acgccgccat cgaccttgcc ggcctgcacc caggcagcgg tggcatcatg
     1081 cgcaccggag tagccgacgc ggctgaagtg gctttccggc ttgatgccat cttgcagcat
     1141 gaaatagcgc ggcatcaggc tgccggaggt ggacgacacc gagccgaagg caaaggtctt
     1201 gcctttgagg tcggccaggg aggtcacgtc cgggttggcg gtgatgaact tgctggtgaa
     1261 ctgcgcatcc tgctcgcgct gtaccagcgg aatggcgttg ccggtcttca ggcgcacctg
     1321 cacgaaggta aaaccgccca gccaggccag gtccagacgg tcggtggcca gggcctcgac
     1381 cactgcaggg tagtcggcga ccggggtgaa ctcgaccttc atgcccagtt gctgttccag
     1441 gtaggcgccc agcggtttga atttgcgcag cagttcagtc ggcgcttcat ccggaatggc
     1501 gctgacgcgc aaggtttcgg cggcttggga caggttggcg gacaggcaca gggcaaagcc
     1561 ggcggcgagc gccagggtac gtttgagcat gaagattctc cggttcaata gcggaaaaat
     1621 tcgaaatggc agaccagcaa ggtgcgcggg cctgcgcgta gtcagggtag tcgacgtggc
     1681 gcgattatag gggccgctgc gctaaaggcc agctttgccg gctgtaggtc aataagacca
     1741 ggtcagcagc gccggttact gaactagcgg cttgtcttgc ctggattttt caactgcctg
     1801 ttagaatccg cgcctgtttt ccgatttgcc gagagcctga ccgatgagcg agccgatccg
     1861 tttgacccag tacagccacg gggccggctg cggctgcaaa atttcgccga aggtgctgga
     1921 ggtgatcctc gccggtagcg gcgcgcagaa cctcgacccc aaactgtggg tcggcaatgc
     1981 ctcgcgtgat gatgcggcgg tgtatgccct ggacgacgag cgcggggtgg tttcgaccac
     2041 cgacttcttt atgccgattg tcgacgatcc ctatgacttc ggccgcatcg ccgccaccaa
     2101 cgccatcagc gacatctacg ccatgggcgg cgatccgctg atggccattg ccatcctcgg
     2161 ctggccggtc aacctcttgc cgccggaagt ggcccgcgaa gtgattcgcg gcggccgcgc
     2221 ggtgtgtgat gaagcgggta ttccgctggc cggtgggcat tctatcgatg ccccggagcc
     2281 gatcttcggc ctggcggtca ccggtgtggt gctcaagcgc catatgaaac gcaacgacac
     2341 cgccacagcc ggctgccagt tgtacctctc caagccgctg ggcatcggca tcctgaccac
     2401 cgccgagaag aaggccaagc tgcgccccga ggatgttggc ctggcgcgcg actggatgtg
     2461 caccctgaac aagccaggct cgcgcttcgg caagctggcc ggggttactg cgatgaccga
     2521 tgtcaccggc ttcggcttgc tcggtcacct ggtggagatg gctgacggtg ccaatctcac
     2581 tgcccagctc gactacgatg ccgtgccgcg cctgccgggg gtcgactact acctggccga
     2641 aggctgtgtg ccgggcggca ccctgcgcaa cttcgacagc tatggcgaga agatcgcacc
     2701 tattacccaa gagcagcaga atctgctgtg cgacccgcag accagtggcg gcctgctggt
     2761 ggcggtcacc ccagagggcg aagcggagtt tctcgccgtg gctgctgagc tgggcctgaa
     2821 cctggcgccg atcggccgtc tggttgaacg acagcgttac gcggtagagg tgctgtgatg
     2881 cgcgacaaca gcaacaacta ccgcgacatc ttcctcaatg atctgccgat gatggacgcc
     2941 cgcgccccgg tcgaattcag caaaggcgcc ttccccggtg tgctcaacct gccgctgatg
     3001 gatgacagcg agcgccagcg ggtcggcacc tgctacaagc agcatggcca ggacgccgcc
     3061 atcgcgctcg gccaccagtt ggtcagcggt caggtcaagg ccgagcgggt tgcggcctgg
     3121 gcggcgttcg ctaaggccaa tccgcacggc tatctgtact gctttcgcgg tggcctgcgt
     3181 tcgcagatca cccagcagtg gttggcagaa gccggtattg attatccacg ggtgatcggc
     3241 ggctacaagg cgatgcgtac cttcctgctg gaaaccaccc agcaggccat agccgagtgc
     3301 gatttcgtta tcctcggcgg cctcaccggc accggtaaaa ccgatgtgct ggtgcagctg
     3361 aacaacagcc tggacctgga aggccatgcc aaccaccgtg gctccagctt cggtaaacgc
     3421 gccagcggcc agccggcgca gatcgacttc gaaaaccgtt tggccatcga tctgctcaag
     3481 cagcgcgccc ggggcatcga acagtttgtg ctggaagacg aagggcggat ggtcggcagc
     3541 tgcaccgtgc cgctggagct gtaccagggc atgcagcgat acccgttggt gtggctggaa
     3601 gatgccttcg aggaccgcgt cgagcgcatc ctgcgcgatt acgtggtcaa cctgtgcgcc
     3661 gaatttatcg ccctgcacgg tgaggaagag ggctttcggc tctacgccga acgtctgctg
     3721 caaagcctga gcaatatcca gaagcgcctg ggtggtgagc gttaccagcg tttgctgacc
     3781 atcatgcagg cggctctgga cgagcagcgt agcggcgcgg ttgcgctgca ccgcgactgg
     3841 attgccgggc tgctgcgcga atactacgac ccgatgtacg cctaccagcg cgatagcaag
     3901 gcggcgcgta tcgagtttgc cggtgaccag gcggcggtca tcgcctacct gaccgagcgc
     3961 aaggctaatc gccaaggctg agctcaatcg cccagcccct gcgctgtaat cagctgctgg
     4021 tagcttctgg cagcttggcg atcaccttga tctcgaactg gaagccatac agccaggtca
     4081 cgccgacggc ggttatggtc gggtacggcg cgttacccca gaactccggg acaaccttcc
     4141 agatcctttc aaacgtcgcc tgcggatcga ccataaagac ggtcacgtcg accacatcgt
     4201 caaagctgcc gccggcagcg tgcagaatcg cgttgaggtt attgaacgcg gtgcgcacct
     4261 gctcctccag ctcaggctcg ggtgagccgt cttcacggct accgacttgc cccgaaacaa
     4321 acaaaaagcc attgctgcgg accgccgggg aataacgatt gcgctcatac agcgcatggc
     4381 gacctggtgg gaatacaacg tcacgctggt tcataagtac ctccatcttg gggccggtag
     4441 cgccccgatt tattaacgat gaagtgactg tagggcgttg cgttaggccg ataaaccagc
     4501 aactctggct aacactgttt gtagaatcca aataatccag tcgatattgg gatagaaaat
     4561 ggaccgtttc gctgcgatgc aggcatttgc tcgggtggtg gaggcgggca gctttaccaa
     4621 ggcggccgaa accctgcaca tgagtaaaac cagcgtgagc cagttggtgc agcagctgga
     4681 ggcacgcttg cgggtcaagc tgctcaaccg caccacgcgc aaggtcaacc tcacggccga
     4741 cggcgcgctc tattacgagc gcgtgttaag gctgttggac gacctggctg atgctgaaac
     4801 cagcctgtct ggcgcctcgg cggtgccccg cggccggctg cgggtggatg tgcccagccc
     4861 gctggccagg atgattctta tgcctgcact gccggcgttt cacgcgcgct acccggaaat
     4921 ccagatcgat atgggcgtca gcgaccgcat cgtcgatctg attggcgaga aggttgactg
     4981 cgtcgtgcgc ggcggcgagc tgaccgatca atcgctgatg gcgcgccggg tcggcgatct
     5041 gcaactgggc gtttatgccg cgcccagcta cctgcaacgc ctcggcacgc ccgcgcaccc
     5101 gcgtgagctg gaggattcgc agcaccgtat tgtcggcttc ctctgggcgc gcaccggcaa
     5161 agccctgccg tatgccatgc acctcgccgg ggagagcatt catatccagg gtcgttacgt
     5221 gctggcggtc gacgatggca acgcctacct ggcggcgggg ttggctggca tgggcgttct
     5281 atggctgccg gactatatgt ccgccccgca tgtggcgcag ggtgagctgc tgccgctgtt
     5341 caccgactgg cgcctcgaac cgatgccgct gtacgtggcg tttccgccga accggcatgt
     5401 cagcatcaag ttgcgcgtgt ttatcgagtg ggtggctgag ctgatggcag agcatgcgcc
     5461 gatcagccag cgggtcctgc cgcgcgcaag ttttccgaag cattagtcca gctcaatgcg
     5521 gccagtgctt ctgcgcctaa gccctaggtt gggaatcgcc accagcgttc gctgacgcac
     5581 agacgcgccc tctacttgca tccaaggtaa agcctcaccg cgccaaccag gctcgtcctg
     5641 cgtatggtcg cgcccgtcat gggtcgtatc cacaccgaca ggagtccagc atgcaaaacc
     5701 caacgttcaa ccccgctagc aatggccaat cggcccccag caaaccgttc agcagcgttt
     5761 cggcttcgag cgtttcccgc gagttccaga attttctcgc cgacctcgaa gacctggtca
     5821 aagaaaccac ctcgctcact ggcgaagagc tggcgcaagc caaggagaag ctcaatacgc
     5881 gcttggccac tgccaaggca tcgctcgaac atatgaacga gagcgttgtg cagcgcgcca
     5941 agcagagcgc cgccgtgacc aacgaatacg tgcatgagca accttggaaa gcgctcgggg
     6001 ccagtaccgt gattgccttt ctgctcggcc ttgccgtcgc ccggcgtaac tagtcgctgt
     6061 tgccatgaac cagaaccctg aagaacaacc atcggctgcc acgcatgcag ccgaggggct
     6121 cagtctgttc gccctgctgc gtttgctgcg ctcggcgggc cgtgcggtgt ttgcccaggc
     6181 cgcgctgcat gggcagctgg cgcgcgtgga atgggccgaa gagaagcagc gcctgctgcg
     6241 catcctggta acgacagtct tcggctttgt ctgtctgttg tgcctgatgc tgctgggcaa
     6301 tgccctggtg ctggcattga gttgggccac cccttaccgc atccatgcgc tcgtgggatt
     6361 gatctgcttg cacggcctgg ctgtcgctat cgcctggcac catgtgcgcc gcctggtacg
     6421 ccaaggcagc agctttgccg cgacccgcgc cgaggtggcc gccgatatcg ccctgatcaa
     6481 gaggaatcta tgagcacgct ccctgcatcc gcgctagaag agcaacgcca ggcgctacgc
     6541 cgcaggttgt tgcagcaacg tcagcgtatt ggctatctgc tggagccgcc ggtgggcggg
     6601 gacaatcagt tcccgcggag catgaccatg cgcctgctga cccggcggcc cgccgtgacc
     6661 atgcgcctgg cctcgcagat ggcgctgttg ctctgcggcc cgcgcattat ccgtaccgtc
     6721 agcggtgcca tgctgctggt cagattgctg cgcgccgtct cggcggagca gcgccggccg
     6781 ccgccccgct aggctcaagc ttgaatgcgc tgcagccagc gctccagctc ctgcgctacc
     6841 cgctccggct gctccaggct ggacaggtgg ccgcaatcat cgagcaccac cagctgcgcc
     6901 ccgtcgattg cagctgccag ttcttcatgc acgtccagcg gggtgatggc atcttcgcgg
     6961 ccgcacaaaa ccagcgtcgg gcaactgatc tgcgccaagc tgggacggct gtcgatacgt
     7021 ccgagcatgg cccgctgctg acgcataaat gctccctgcc ccaggctgtt ggccatggcc
     7081 gtcaccacct cggtcaagtt ctcatcagcc tgatgctctg gctgcagcaa cttgggcagc
     7141 aggccggcaa taactgcagg gaactcgtgt tcggctttag ccatcaacgc ttggcgcccc
     7201 tgcttggttt cgtcgctatc ggcatgcgcg ctggtatcca gcaacgccag gccaagcacc
     7261 cgttgcggtg cctggcgcat gatttccagg gcgacatagc cacccagaga aagcccggcc
     7321 agggcgaaat tttcatccgg cgcttgatcc agcaccttgc tcgccatttc ggcgatagag
     7381 tcggcctggc tcagatcggc caccgtgaca tacgccagat cagccagcgc cgccgcctga
     7441 tgttgccaaa gacgggcatc gctaagcatg ccgggaagca gaagaagatg cggcttgtgc
     7501 atggtaaacc tcgcgtaatc gttgggtgga gctgcttaag gcagcgcgca gataccttca
     7561 cccaacatgg gcgtaaatca cgtggcttat ctgtgcggcc ccgcacattg gcgggtttgt
     7621 ttcgctcagc gccggtccat cggagcgctg tcatagccag tactcccaga gccgaccgaa
     7681 caactccttg gcccgcatat cgagtggccg cgcctgccag tcaggcaggg tgatttctcg
     7741 ggacttctcc aggtcagcac gaaacgctgc gcgcatcttg tcgccgaaat cgttgccgag
     7801 cacgaccgcg ttgacctcct ggttatgcag aaaactgcgc cagtcgaggt tggtcgaacc
     7861 gactgtcgac cacacgccat cgatcactgc cgttttcaca tgcagcaagg cttcgtggcg
     7921 ctcgtagata cgtacaccgg cccgcaacag ttcgtcgtag taggcgcggc cggcatggaa
     7981 caccagccag gagtcggtgc ggctgggcag gatcaggcgc acatcgacgc cgcgtgccac
     8041 ggcctccttc agggtggcca gcagctgtgg gtcgggcacg aagtaggcat tggtcaacca
     8101 gatctcgcgt tgtgcgctgc gcagcgcgga aatcagggtg acgtaaatca gactgtaggg
     8161 atcatccggc gagctgccga tggcgcgcac cacctcattg ccgcgcgcct cggccactgg
     8221 aaagtactgc gcggtgttta gtggcgggcc tttttgcttg gcccaggttt cgataaacag
     8281 ttgctgcaat tcggccacca ccggcccttc gagacgcaga tgggtatcgc gccagggcac
     8341 gtcggcggca ggctcagctt ggttgcccag gcgtaacgaa ctgccggagt acacgctgct
     8401 gatattgatc ccgccgagaa aggcaatgcg cccgtcgacg atcagcaact tgcggtgatc
     8461 acgctggttc agcgtccagc cggccttggc ggtgagcggg ttgatcgggt tgaactcgac
     8521 gacctgcacg ccggtctcgc tcaggcggct gaaaaagctc gtcgcggtgc ccagggtgcc
     8581 aacgctatcg tggatcagat tgacctgcac gccggcctgt tgcttggcga tcagtgcgtc
     8641 tgcaaagcgt tggcctattt catcgccatc gaagatgtag ctctccatat tgatatggtc
     8701 gcgcgcaccg gcaatcgcct ccagcatcga ctggtaggtg cgtggcccat cctgcagcag
     8761 ctcgacccga ttaccggtgg tcaatgggct gccggcaatc gattcctcaa tggccaggtg
     8821 caggtcgaag aacgtgctgg atgcgtcgcg ggcctgcagc cgttcgatga tggcctggcc
     8881 gcgcgcggtg gacagcggac cgttggcgtc gtccagtgct accggcgcac ggttgtgcgc
     8941 catatccggc actagtgtgg ggagtgccgc acagccagaa aaaagtgcca ggcatacagc
     9001 caatggcacc aacagcaacc aacgcctgca tgacatgcaa aaactccagg cagtagcggg
     9061 gcgatgcaat cctgccgcca ggctagcagg gttcggcgcc gcgcaattgc gggggcttgt
     9121 gtacaagaca gtggcagccg agggcatggg cgtcggtgcg ctacagcgca taagggcagc
     9181 gcaggatcgt gcgtctagac gacagtgtcc tggttcagcg gcaagcggca gcgatccacc
     9241 ttccaccagg tgggcagcag cttgcggatc gctggctggt tgaagcggtc atcgatcagg
     9301 tagaccacgc cctggtcctg cacggtgcga atcacccgcc cggcagcctg taccaccttc
     9361 tgcaaacccg ggtagaggta ggtgtagcca tagccgttgt tgctaccaaa catcagctcc
     9421 atgcgttgct tgatctcttc attggccggg ttgatctgcg gcatgccgag ggtggcgata
     9481 aacgcgccga tcagcctgtc gccaggcaga tcgatgccct cgccaaaggc accacccaag
     9541 acggcaaaac cgatgccgcg gcccttactg gcgaaacgat cgagaaaggc ctgacgctcg
     9601 ttttcatcca tctgccgcga ctgctgccag accgggatct gcggatgcgc catctgcagc
     9661 tcctgcagtg ccgcttgcag gtaggtgtaa ctgctgaaga aggccaggta gttacccggt
     9721 ttttccgcgt actgacgggc aatcagcgcg gcgatgggcg ccagcgagaa gtcgcgatgg
     9781 gcgaagcgag tggacaggtt gctgacggcc tggacctgca gctgctcggc gctaaagggc
     9841 gactccacat cgatccaggg tgtttcctcc ggcaggccga gcaggtcggc gtaatagttg
     9901 gccgggctca gggtggcgga aaacagcgtg cagctgtgcg cctcgtcgaa acgcggcgca
     9961 agaaacggcg cgggcacgat attgcggatg cacagggtcg acactgcctg gccgctgctc
    10021 tcgtcgcggc tgatatcgaa cagcgagtgc gcaccaaagg cctcggccag gcggcagaac
    10081 agcatggcgt cgaggtagaa gtgcagcagc tcgccggcat tgccagccgg ctgttcgctg
    10141 aggtgatcgg taagggtgct gaccgccttt tgcagcgcca taacgaacag gtccggcgcg
    10201 gtggggtaaa cctgataggc cacttcctga tcgcgctcca gctggcgcca atgccgactg
    10261 atgcgctcca gtggcgcctt catcacgttt ggcgcgatac gccgtagcgc ttcaaagcgc
    10321 tgctgattga gctcggcggt gtacatgccg cgcgcccgtt caatcaggtt gtgggcttcg
    10381 tccaccagca gggtgatacg ccattcattg agtagggtaa ggccatacag cagtccgccc
    10441 atgtcgaagt aatagttgta atcgcagacc accacatccg cccagcggca cagctcctgg
    10501 ctcaggtaat aaggacagat ctgatgcgcc agggcggttg cgcgcacgct ctcgtggctc
    10561 agccattggc cttcgagcgc tgcttgacga gctgcgggta aacggtcgta aaagcctttg
    10621 gccagcgggc aggagtcgcc atggcaggct ttgtccgggt gctcgcaggc cttgtcgcgc
    10681 gccacatgct ccagcacccg cagcggcatc gcctgttcct ggctgcgcag agtggccagg
    10741 gcatccaacg ccaggcgccg gccaggggtt tttgcggtga gaaaaaacaa gcgatccagc
    10801 tgctgctcgg ggaaggcctt gagctgcgga aacagggtgc cgagggtttt accaatgcca
    10861 gtggtggcct gggccatcag gcaatggccg tcacgcgccg cgcggtaaac ctgattggcc
    10921 agctgacgtt ggccgctgcg aaactcggca tggggaaagc gcaggcggag cagggcttca
    10981 tcgcgttggc ggcgatgctc acgctcgtac tcggcccagc ggatatagcg gctgcattgc
    11041 agctcgaaaa actcccgcag ttcatccgcg ctgacagtca ggtgaaaggc ggtttcatgc
    11101 tgggtgataa cgttgaaata gaccaccgcc agattgatct cagccatgtc gcgttcctgg
    11161 cagagcagcc agccatatat cttgacctgc gcccagtgca gcaggcggtg gttttccggg
    11221 atacggctga tatcgcctct gtgggtcttg atctcctcca gcaggttttc atccgggttg
    11281 taaccgtccg cccggccgcg tacctgcaaa ccttgataga cgccgctcaa aggcagttct
    11341 gcgtaatagc cggggccacg ccgggccacc acgatggcgt ggccggtcat gccttcctgg
    11401 gcggtgggcg agggggtaaa gcgcaagtcg aggtcaccga ccttggcggt aaactcgcac
    11461 agcgcgcgca ccgcaacctg atagttcacg cgtcggccca ctgcacgtag cagacatcca
    11521 ccggcatgcc gtgctcggcg cagaaggcca gccagcgccg ctggttgtcc tgcaggcggt
    11581 cgcccgggcc tttgacttca atcatccggt agcgctgctc ggccggccag aactggatca
    11641 ggtcgggcat gccagcgcgg ttggccttga tatccaccaa aaggcgtttg aaccaggcgc
    11701 gcaggtgcgc ggcgggcagg cagtcgagtg cttgttccag caagctctca tccagcgccg
    11761 cccagaatac gaagggcgac tggatgccga atttggccgc gtagttttcc cggattacct
    11821 gcttgtaggt atccgaatcg agatgcgcga ggcaactggc aaacaactcc tcgcgctggg
    11881 tatggaagtc agcgctgaac agatccaccg gcgcggtgtg gaacgggtgg aagaatgccc
    11941 ccggcactgg cgcaaagatg gccggccagc agagcaggcc gaacaggctg cagatcaggc
    12001 tgttctcgac gtaatgcacc ggcgcgtcgg gctcgtgcag gtgggctttg acagcaaact
    12061 cgacactctg cgcctcgggc cttggcaggc ttagatcaat ccgtgccggc ggtactggct
    12121 tggccttgcc gggctgcggt tgcccaagct ggcgggtcag ccgggtcaat gcacgttcca
    12181 ctgcctgggc ttcggcatca ttttccggtg cctggatggc ttgcagcgtc agcgcatagg
    12241 cctgttcggg gcgacccagg cgctccaata cgcgaatctg ccgcagccgc gcgccgacat
    12301 accggcagtc gctataaaac gccagcgcac cttccaggtc agcctcacgc tccagttgct
    12361 ggcccatgcg gaacagcagc ttgttacgac gcgcggccag gaagggcgta tcgcaggcca
    12421 cggcgttgat ctgctgcagc acgttggcaa gcggctcgcc ggcctcgaac tgttcacggc
    12481 tgcgctgcag gtgcaggtag cagtcgacat ccgcgcggtg acgaaaggcc cgtgaatcgg
    12541 ccgtcagcgc tacccgctca tagcgaaaga taccgagctc ggcgagcacg aactcggacc
    12601 agtcctgatg cagattgccg aagaacatca agcgcaggcg ctcgcacagg gcgtcgatgg
    12661 tcagggagac gatctgctcc tcactgcccg gacaccaggc cgcaaaggcg cgcggctcgg
    12721 cgcaggcctc ggcaagctgg gccagcagcg cggatttgtt ctgcgacttt ctgcttgtgg
    12781 ggcccgccag gtgctgcagc acttcatcct tgcgcagcag cttgaacagc tcctccaggg
    12841 acagcttggc gtcatgccgc acccaaccgg cgtcgagcag cggcgctgcc gcctggcgcg
    12901 cgcagccgat ctccgcataa ctcagcttgc tcaggcgaaa gtggctgccc ttgcgcatga
    12961 tcatccgcac cagcagagcc tgggcaggcg tgtccatggc gaagaactgc cggacaaatt
    13021 catgctcggc ctcgttcaac agatcggcgt agcgctcctc cagccaggtg agggcctgac
    13081 ggaaattcgc caggtagtag aagtgcggat cgagttgcga ctgcatgggc gtgtcggtgg
    13141 ctgtgtgcat attcaggtat cgcgcctcgc caggcgggca atgcaaatgg ctggggaggg
    13201 cgcagggaat gcggttggga taggggccgg gtagagttta gcctgtgctg tttatctata
    13261 cagcattaat ctgcagctaa ttgcccccgc ttgaggacaa gcatggctgc cccgtagggt
    13321 gagctgtgcg cacaaaaaaa tgcagccggc cctggtgcgc acggcgcacc ctacctattg
    13381 cgagccgcaa acgcccaatg aaagtggaat tcgccgtttt ggattggcta cgtatccgtt
    13441 aactgttgcg cgattgtccg gcttacgccg attagtcccc tcgccccttt ggggagaggg
    13501 ttagggtgag gggggctatt caagcctgca cactggcgtg tttggcaacc cccgcttctc
    13561 agttcggaag ggagcaaatc gctcctggca atacaacgta cacagccttg gaatttatag
    13621 accggaaggc gagcccacat gccccagttc ccgggatata tcgtggctgg cggcgagcag
    13681 ggccgtgaga aagccctgct gagcaatctg cgtattcatt accgcatccg ggccggacag
    13741 gttgatggcg gccactatgc tgccgctgtg gtcgcgaata ccggtggcga tggcggtgga
    13801 gtagtcggag cggtgcagca cccagccgcg ctggtgatcc tcggcgataa gctgttgcag
    13861 ctccggcagg gtgcgcggtg ccggcataga aaaggggacc gatttatttt tgagcgtttc
    13921 cacgctcagg cgtgggaacg ctgcttgagg gtagtgctat ccatcaatca agccaacagc
    13981 caaccccatg gctatcgggg cacttcagct tgctggcgat caaggctgca tacttacgcg
    14041 ggaaaatggg agcgatcaat gtctgtctat gcggtggact cagccgagta ggatgggttg
    14101 agcgcagcga tacccatcaa ctaggtcatc cctaaccgca tgggtatcgc gttgctcaac
    14161 ccatcctacg gaccgatacc gtagggtgga tcacgcttca ccgatccacc gggcggcgtt
    14221 atggtggatg aaaagagcgc catccaccct acaaactagt gctcccaaca cgccaaggaa
    14281 caggcaaccc aaatggctca agcatccgct cctgaaaata attactatcg ccctgcatcc
    14341 ttagccggcc ttataagcaa tgactgagaa aactgtcgct ccagccgaag cccctcagag
    14401 ccaagagcct aggcaatatc cgctgtttgg caacgacgcc gacagcgcac cagcgagctc
    14461 tgcgagcgac ccgatcgata agtcattcag ccgatacgtt gtgtatgtgg acgaaagtgg
    14521 cgaccacggg ctggaaaacg ttgatcccaa ctatcctgtg tttgttctcg ccttctgcgt
    14581 atttcataag aatcactatg cacagcgggt cgtgcccgcc attgaggcct tcaagttcag
    14641 gcacttcggt cacgacctcg tcgttttgca tgaaaccgat atccgcaaag aaaaaggcgg
    14701 ctttcgattt aatagccgcc agcacagaga gcttttcctg agcgaactca ctgacatcat
    14761 tgaaacaagt aacttcgttc tgatcagctg cattatcgat aagcaacggc tacgcgaacg
    14821 aaccagcaac ccctatcact tggcattgag cttttgcttg gaaacgctct atgagctgct
    14881 agaagagaaa cagcagcatc agcctactac gcatgtcgta gtggaatgca ggggcaagcg
    14941 ggaggacgcc gagttggagc ttgagtttcg gcgagtctgt gcgggggaca ataaattcgg
    15001 gaagtcgctg cctttcgaga ttgtgtttgc cgacaagaaa actaattcat ccggcttgca
    15061 gctggccgat ttggtggcac ggccgattgg actgtcagtg gtaaagccgc aacaaccgaa
    15121 ccgggcgttc gatctgctta agagaaaatt cttctgcagt ggcggacgta tgcaggtggg
    15181 tatcggtttc gacggatggg ggctaaaaat tcatcctgct ctagaaagcg aaaggccccg
    15241 gtgatcgcac cgaggccctg acgccgaccg ggaaccccca gtccatttgc ggtaattgta
    15301 ggtttttacg cgcgttaatc gcaagcgtgc tctacaaaga aaacgggata caggtggcct
    15361 aattgcagtt ctccgacgct gaatacattg cccagcccca tcacgctagg tgtagagcat
    15421 ccaggtcagc gcaattgccc tttccaccgc gccaacccat gaactgcagg cctaccgcac
    15481 aacggaaagt gagtagtcaa tcgtgagtaa tttccagttc cttacctccg aattcaaaaa
    15541 cctgtttgaa ccggccaagg gcgcggagca gttgatgtat tcggacccgc gtgcttgctg
    15601 catgcgcacc tgccatgccc tggagcaatt agtgcattgg ctgtacgagc atgaccgcga
    15661 tgccaacgcc attgtggcgg tgattgcggc catcaaacgc agtgcggcgg cgtaatggcg
    15721 tatcagccgc cctttacgct gaacggcagc attctgtcgt tggtggcgga gattgcgcaa
    15781 agcgtcggcc gcttgagcgc gcagccggag catgccaatg cgttgcggtt gcggcggatc
    15841 aaccgtatcc gcaccgtgca gggctcgctg gcgattgagg gtaatacgct gagcgaggag
    15901 cagatcaccg cgatcctcga cggcaagcgc atcattgcgc cgcccaagga tgtgcaggag
    15961 gcgcgtaacg cactggccgt ttacgagcag ttggaggctt ggcagcccgc gaacgaacag
    16021 catctgctca atgctcacgc gctgctgatg aaaggcctga tcgacgatgc cgggcattat
    16081 cgccaaggcg gtgttggcgt gatgaagggt gatcaggtgg tgcacatggc tccgccggcc
    16141 aatcgcgtca gcaagttaat gcgtgacctc ctgcagtggc tggagacaac cgatcagccc
    16201 ccactgattg ccagttgcgt ttttcactac gaattcgaat ttatccaccc ctttgccgat
    16261 ggcaatgggc ggatgggacg gttgtggcaa accttgattc tgagccgttg gcagccactg
    16321 ttcgcgcatg tgccggtgga aagcctggtg cacgcgcatc aagccgagta ctacgcggct
    16381 atcaatgaca gcacccagca agccgatagc gcgccgttta ttgggtttat gttgagcatg
    16441 ctgcgtgatg cgctgctaag catgaccccc caagtcaccc ctcaagtgcc cccccaagtc
    16501 gctgagctgc tcaggcatat ggtcggcgag gtcaggcgtg agacgctgca agctgcgctg
    16561 ggcttggccg accgcaaatc tttccgcgag cgttacctgc gtccggcgct ggatgcaggg
    16621 ctgattgcca tgactcgtcc ggacaaaccc aatagccgct tgcagtgtta ttacctgaca
    16681 acgctgggta agcagattcg gggtagtgct gcgttgtagt gcctgtccgg gcgcggcttt
    16741 gtatcccaaa aactggcgct atttcgcaat ccgagcggcg ctgacagcct gcacaaatca
    16801 gcccaatgcg cggcataacg cgaccctagg gtaacccccg gaaacttggc acccgcgcgc
    16861 tgtataaagc tatgggatca cttgctgtgt tctggcgaac tgggtcctgg accgtgaccg
    16921 cgaggcccgg ctacgcccca gatgaccagg ccaataatcg gcacgatcca tatcagcaag
    16981 gcccagagtg tttttgtacc gaccgtttta tcgctacgaa aaacactgat aacggcccac
    17041 aggttcacca aagcgaagag ggcggccact gcgattgcaa agtacatggc tgaatccggc
    17101 atgctctacc tccttgaaga cagcgctaat cacactccca ggtctgcctg tgttgttctt
    17161 gcgccggcag tgtcgactga catcattgat ttcgaggaac cgcctggagg cgaagttcac
    17221 cccttcagag cagcggtagc gcggtcagcg ctggcaggtt tcgcttagcg aatgttcagc
    17281 gctggccgca gcgatccgct tccagcgatt gcgccagctg ttgcaggtag tcgccaaatc
    17341 agccgcgtgc gcgcgaatcg tggcgggcgc tggtggcgac gctgttgctg ccactccaga
    17401 cgatgcgcgc gccgacttcg ttcagtgact cgatcagccg cacgtcctca tgggcgcgca
    17461 gcggtggaaa cccgccggca cgtttatagg cctcaacgca gatgccaagg ttggcgccgt
    17521 gtatgtggcg gtgactgtcg ccgggctggt aacgctggtg ataggcgcgt tggacggcgt
    17581 cgtcgtagcc ttcccaatcg tccagatgaa ccgtgccgca gaccacctcc gcgtcgtgtt
    17641 gcagctgttg caccagccag tccgcagcca cgcagctgtc ggcatcggtg caggccagcc
    17701 agcgcgcgcc gaggctgatg gcatgagccg cgcccgcgcg tcgggcccgg ccgacgttgt
    17761 gcgccgcgat gctcaggctg atgacctgat gctggcgggc gatctgtgcc gaggcgtcgc
    17821 tgcaggcatc cagcaccacc acgatgcaca ccggctcgcc ctgcagatcg gagtggcggg
    17881 cggcgttgtg cagcgattgc aggcaacggc cgagcagcgc ttcttcattg tgggcgggca
    17941 ccacgacacc gatcatcggg cgacctcgtg taggtttatg gcgaacgctg agacgctcca
    18001 ggcttccagc acgaagtcag gctcctgatg caagacgatc cgctcgaacg gcagtatctg
    18061 ccgcagcaga cggtgcacct gctcgccgtc gagcgggctg ccttcgatgg gatgccgcca
    18121 gtgacaggca agcagatggc cgctgggggt aaggctggcg atgcagtgtt gcagcaggtt
    18181 ggtcagcgcc tgctggtcaa ggtagtagcc gacttcgctg atcacgatca ggtcaaagca
    18241 ctgctccggc cagcactgcg gcagctgccc cgcgtagacc tgcgcatggg cataaccggc
    18301 aaggcgctcg cgggccaacg cgaccgctcg ttggctggca tcgcagcaca acaggtgttc
    18361 gcagcgctgc gccaactctg cgctcagctc gccgttggcg cagcccagct cgaaagcgcg
    18421 ctggtaacgc tggcgcggca gggaagcgag ggtcagttgg cgtttgcgca actcgtacca
    18481 gcggctgcga aagtgccagg gatcgtcgtt ggcggcgtac agcgcatcga aataatcggc
    18541 agacaggctc atcggaagta cacctcatag ggctgggcga aacgcgcaag ggtgcgctcg
    18601 gtgaggatcg gcggcgcacc ggtagacgga tcggccagca gctgcgagcg gtgcgcgtgc
    18661 agggcaaaac gcttgcgagc cagggtctgc tcgccgagcc tgagcttgca ggcgcgttcc
    18721 caggggatgc gtgggtcttc cggtgtcgcc cagtgccagg cccagaccgg catctcgtaa
    18781 agcgtcgcgc cggtggcctg tgcaactgct gtgctgacgc gcccaaccac ctcatgatcg
    18841 cagtgaccgt cagcgcgcca ggtggtgatg acatggctgc agccttgcag cgcctgctca
    18901 aggtgcgcgc gcagacgtgg ctcatctagc gccacgccgc tgtcgggcag atgcagccgt
    18961 gtccactcga tgcgctgcgc cgaaatgccc aggcactgca atgctatgcg gctttcctgc
    19021 tggcggattg cgcgcagcgt ggccaccggc cagagcgtgg agttggggtg gctgccttca
    19081 ccatcggtga cggcgacgat tctgatcggt gcatccctgg ccgccagttg cgccagcaac
    19141 ccgccgcagc ccagcacttc atcgtctgga tgcggcgcga caatggccag ccgcgaaccc
    19201 ggggcgatca gctcgtcgag cgcaaccacc ggcagcgcgg ccagccgctg cgagctggcc
    19261 caggcttcgg ctgaggtgcc ctggccctga ataaggttgt cgtcttcgct cacagattcc
    19321 agtccttgtc gctgtgcagc agcagttcgc ccagccgttc gagatcgcgc tcggcatggc
    19381 tttggcgcag gaataccggc agatcggcga gcaagcgggc gaagtgcggg tcacggcaga
    19441 aggggccagc gcccagcgcg cggccggcgt ggcacatgac ttgctccagc gccgcttcaa
    19501 cctgggcgcg caccaggcgg gcagggtact cagccgcggc agttggctgc ttgtcgatcc
    19561 agctggcgca ggcccgtagc gcctgtttcg cgccgctcag cgccgtatcc agcgcgccca
    19621 gatgcgccag cgcgtgcggg tcgctacgca tgtgctgctg cacatgccga gctatcgcca
    19681 ccccggcacc gtaccaacag gcggcgatgc cggcgccgcc gtgccagaag ccggggcgtg
    19741 cggtgtaggc ccctggctcg ccaatacagc gggcactagc gccctcgaga cggatctcga
    19801 agctggcggc cgctgccatt cctactgcct gccaaccgtc gcgggtcacc gtgacgccag
    19861 gctgattcat cgccagcgcc accaactgcg agcgtccctg ttcatcccag accgtcacca
    19921 gcgcctgatc gacatgcaag gccccggagc accaggcctt gcagccgtgc aacgtgacgc
    19981 cgtcagcgtt gcgcgcggtg atttccaccc gcgcctgcgg cggttccgcc gcccacacgg
    20041 cccatagctc cgtgttctcg tcactggcgc caagctcgcg gagaatggcc tgggcatcgg
    20101 tgtgcgcctc gaacagtttg agcagcgata aatctgctgc cgccacctcg gcaagcagct
    20161 gccagcgccg cagcgtttca ccctggcccg gcgttggcag ctgatccagc ccggccgcca
    20221 tcagcgcgcg catttgccgt ggcacagcat cgttggccgg ctcgcacgcg cgcctgctga
    20281 aggcctctag tgcgctagcc agttgcatgg gctgttcagc ggccacaagg gcagattgct
    20341 tgccggtcac cgtgtgctcc tgtggatcac gccaagcttg tcccacatgc cacggttgag
    20401 ccgttgctga ttggcgtatc cgtgccaggg gtctcgtgca agttgctcaa ggcgttgcct
    20461 gaggttggcg acggtccact gttgggcgct gcgcacctga tccagctcgt cacggctgat
    20521 cggcaccgaa accggcagcc ctggtctcgc ccgtaccgag taggccgcga cggtactggc
    20581 gccgcgcgcg ttacgcaggt agtcgataaa aatcttgccg acgcgattct tcggcccaga
    20641 cgtggcggta aagcgctccg gcagctgttt ggtcatgaac tgcgcgatgg ccttggcgaa
    20701 ggccttgacg gtgtcccagt cggcgcggcg cgccagtggc acgacaatgt gcatgccctt
    20761 gccgccgctg gtcttgagaa acgcctgcag gttcaattcg tccagtacac ccagggtcag
    20821 gcgggtggct tcgagcatgc tgcgccatgg caacgccggg tccgggtcga gatcgaggac
    20881 gaaacggtcc ggagtgtcga tgcgcgtgct ggtggcgccc caggtgtgca gctcgatgca
    20941 gcccatctgc gtggcgccga tcagcgctgg caggctgtcg atttgcatca gcgccgcgtg
    21001 gccgggatcg agcttcgggt ccaggtgctt gatctgtgga atggccagat gctcggcatg
    21061 cttctggaag aactgctcgc cgctgacgcc ctctggcgca cgcaccagcg acacagggcg
    21121 accggccaga tgcggcagca gccagtcggc cacgctctcg tagaactgcg ccagttgcag
    21181 cttgctggtg ccgctttgcg ggtcgatcag tcgttgcgca ctgctgatcg ctacgccggc
    21241 gagcagcggt ttgccggccg cgctggcggg ctcggcgttt ttttccggct tggtcgcgtc
    21301 aggccactgg ggatgttccc ggatgatctg gctggccggc ttgtcgctgc gcagggcgat
    21361 aaaactggcc tggcgcacga tgccttcgcg cgtccactcg gcgaactcca cttcgcccac
    21421 cagctccggc ttgatccagt tgacctcgcg ggcctgcgcg ctggtgagtt tttgcgccag
    21481 tggcgaatcg gcctgcgcca gttgctgcaa gcgctggtgc agcgccttga gcgccgtttc
    21541 gtcgaagcct gtacccacgc gtcctgcata gcgcaggcca gcgtcgtcat tcaccgccag
    21601 cagcagcgcg ccgaaaccgc tgcggctgcc cttgggctgg gtaaaaccta cgatgacgaa
    21661 ctcctggcgc aggcggcact tgagcttgat ccagtcggcg ctgcgccgcg acacataggc
    21721 gctgcccacg cgcttgccga tcacgccctc cagcgacatg gcgcaggcgc tgtcgagaat
    21781 gtcctgatgg tgcgcgttga aatcggcgga gtaccgtagc gggcctttgc gcgtgctcgg
    21841 cagaagctgc ttaagcaggg cgcggcgttg ctccagcggg gtttgccgca gatcctcgcc
    21901 gttgataaat ggcgcgtcga acaggtagta gacgaggtca tggctgcggc cgatatcgaa
    21961 agcattctgc agctgctgga agtccggcag gccgatgtcg ttcagcgcca ccacctcgcc
    22021 atcgagccag ctgtcgcgca ggcccagctt ggccagcgcc tttgcctgca gcggcaggcg
    22081 ctcgctccag tcatggccat tgcggctgaa cagacgcacc tcgccgtcgc tgatgcgggt
    22141 gaggatgcgg tagccgtcga acttcacctc gtacgtccaa tcgccttccg gtggccggtc
    22201 ggagagggtg gccaactgcg gtgccaggct ggcaggaaag tcgctgcgac cggctgcggg
    22261 tttgcgcgtg ctagcggctt ttttgccctt ggcggtcttg ctcggcccgc ccaccagcgc
    22321 gccactgata acgctctgtg gctgctcgtt gacgatgtcg tattcatcgc tggagcgcgc
    22381 cgtatcgtca cgttccttga tcagcagcca ctgttccttg tcaccgctgc cgcgcaggcg
    22441 ggtgcgtacc agcgcccagt cgccgctcaa cttctcgccg atcaagctga acttgagctt
    22501 gccggccgca taggccccga ccgggtcgtc atgcggctgc cagatgccct gatcccagac
    22561 aatcacatcg ccggcaccgt aattgccctc gggaatgctg ccttcgaagc tggcgtaatc
    22621 cagcgggtga tcctcgacat gcaccgccag gcgcttgtcg cgtgggtcca ggcttgggcc
    22681 cttgggcacc gcccaactct tcagggtgcc atcgagctcc aggcgaaagt cgtaatgcag
    22741 gtggcgggca tcgtgcttct gaatcacgaa gcgcagggcg ccggccttgc tgccacgtct
    22801 gcgcggctgt tcgcggggtt cgctggtctg ctcgaagttg cgtttgcggt tgtagtcact
    22861 ctgcgccttg gccatgctcg tcgctccgcc ttagttgcgt ttggtcctgg ctgcgggctt
    22921 gtgcgagccc gtgcttttgc ccggcgcctt gcgcgccggc ttgccaccga ggctgcgctt
    22981 gagcagttcg gtgaggtcga tgacatcggc gctgcgccgg gtgtcttcgc cctcgacgcg
    23041 atccacttcc tcgatcttgc cggccttggc cttgcgcgcc accagctcca tgatgcgatt
    23101 ctgaaactca tcgacgtagg cgtccggttg ccagtcgccg ctcatgtcct tgatcaggcg
    23161 cttggccatg tccagctcgc tcttggccag cttcggtttg ctcaccgccg cgcccagttc
    23221 cagtgacgcc agactgcgca cttcgctggg ccagcgcagc atcactagta ccagggccga
    23281 ctcaagcggc atcagcgcgg ccagatgctg cttggtgtgc agcaccacgt gggccagcgc
    23341 caccttgttg gtgctgctca gagcctcgcg caagagtgca tagacctttt ctccacggcg
    23401 atcgggggcg aggtagtagg gcgtgtcgat gtgctgcaac ggaattttct cgctgtcgac
    23461 aaaggcaaaa atatcgatgg tctgggttgc ctcggggtga gcagcacgga tttcctcagc
    23521 gctgagcacc acatagcggc ccttctcata ggccacgccc ttgacgatat tttccttgct
    23581 gacttccttg ccggtgacct tgttcacccg cttgtagccg accggctcca tgctgcgttt
    23641 atccagccag tcgaagtcca cgccctgcga cgcggtggcg gaggtcagtg caacggggat
    23701 atgcaccagg ccgaaactga tggcgccttt ccagatggct ctaggcatgc tggccgtctc
    23761 ccgccagcga ctcgggtacg taatggaaac tgccgcaacg gctgcagcgc tgacacagca
    23821 gggtttccct gcccatgcgt atggcggtgc ctgacgacca gacacagcct gcgatcaagc
    23881 atcgaatggt cattttctac ctccaacggc cagttggccg gggcgtctgt tcaaacagtc
    23941 gaccgctggc aggcgcagaa ggttttactc aaatgccaag cggcacgcac cgaaagccgc
    24001 tacaagcgga cgacgataga cagtcagcaa cggatctgcg cggtgaggat tcggccgcta
    24061 tggcctggtt tcggtggcgg tcggcggggt ggtgcggggc cagaggggta ggagggggga
    24121 aataacctga actcaggcca aaaccctcgg gtcatttatt gtgggcccag tgaatggggt
    24181 cagtaccctt ctcggcgacg ttacttgccc tcgcgacaag catgtgccga acacaaaaag
    24241 cattaataag cggagaacac tccaatgaaa ggattatccc tatgtagcat cgtcgctatc
    24301 gccatgttgg cttccccggc ttttgccaca gacatctgca ccgtcaatct gcagaaactt
    24361 tcggacagca aggccgcact cacaaccatg acctctcccc tcaaggagca agtcgaagaa
    24421 cgtattaagg ctgccgaaga ggcgcaacgt gcgggtgacc tcgaaacctg tggcactcat
    24481 gccgaaaaag ccctgatgct gctgcaacgt cctagcaagg atcgcagtac cgatgcgcaa
    24541 taagtcggac gaaaaggggc tgatagcgcc tgttgccagc ccctccattg catagttttt
    24601 ctgcgcgtta gcgtcacgcc ttctagagtc aatactcaac cctagaaagg tgatagccat
    24661 gtatcgttta gctgctttcc taaccgccgc acttctcagc acctcgctct gggccatgca
    24721 ctgcccagcg gatatggcga agatcgacgg cttattgaag accgacccac cgagcgatgc
    24781 cacggtactc gcgcaagtgc agcaactgcg cgccgaaggc gaagcgctgc acaaggccgg
    24841 taaccacggc gagtcggtca aggtgctggg tgaggccctg caactgctcg aggccagcga
    24901 gtagcctgag tcacttgcga gtgggctaaa tgcggtaggc gctatttaag cgcctgtccc
    24961 agcaagctaa gtgccacgcg gcgcacctgg gccaggtcaa tcggcttggc caggcaggct
    25021 tgtgcgccgc gtcggcgcgc ctcggcaaac acagcctcat ccgccgccgc actgatcacc
    25081 aataccggca ctgcctgcag ccgagggtta aggcgcatgg cgtcaagcac ctgcataccg
    25141 tcgccatcag gtaggtccag atcaagcagc agcagcgtcg gtgtgcaatg ttcaagctcg
    25201 gccagcgccc gctgaaccga gccggcgcca cgcacctcgg caaactcacg cagcgcttcc
    25261 tgaaccacct tgaggcaggc cgggtgatcc tctacacaca gcacttcggc caactgcttg
    25321 ggctgcgctg ccacggattc cacatcggca gccgtctgtg ctgccggtgc cgcagcgccg
    25381 ggcaggtcga tccagaagcg gctgcccgtg ccggcgctgc tgctaaagcc catctcgcca
    25441 gccatcaagg tggccaactc acggcacagc accaagccga taccggtgcc aggaatgctg
    25501 gagttctcgc gccccagccg ttggaacggc tgaaacaggt tggcctgttg ttcggtagtc
    25561 agccccgtgc cgttgtcttc gacccacaag cgcacgcagt tggaacgcac ctcatagccc
    25621 atgcggatca aaccctgcgg gctgttgtac ttgatcgcgt tggacagcag gttgagcacc
    25681 acctgacgca ccctgcgcat atccgcctgt acatacaggc cggcgtcggc ttcagccaac
    25741 acctgcaact gcaactggcg ttgctgcact tccggctgca ccagctcggc gcagctatgc
    25801 agcagcgcgc cgacttccac gggttgcaat ttgagctgct gacggcggct ctcgatgctc
    25861 gacaggtcga gaatgtcgtc gaccaatgag gtcagatgac gactggcatt gacgatctcg
    25921 cgggcgtaat cggcttccag cttggcctcg ggcttgtcct gcgcttccat ctcaatcaac
    25981 tggccaaagc cgtaaatcgc attgagcggt gtgcgcagtt cgtggctcat gctcgacaga
    26041 aagcggcttt tggcctggct ggcggcctgg gcgtccaggc tggcgcggcg caggtcttcc
    26101 tgcacctgct tgagtcgggt gatgtcgaca tgggtgccgg ccacgcgcag ggctcggccc
    26161 tcactgtcac gctccagcac cttgccgcga ctgagcaacc agacgtactc gcccagcgca
    26221 tcggcatagc ggtattccgc ctcgaaacga tcctcgccac tgttggcgaa cagttggcgc
    26281 agatgacgaa tgcgctccag atcattgggg tgcacgcgct ggttaaattc actgaagctc
    26341 atgttcgagt gggtcaggcc gaagcgcctg aggaaacgct cgctgagggt gatgctcatc
    26401 gtctccgcct cgacctccca gaggctttcc gtggtgctct ccagcaccag ttggagaatc
    26461 cgctgcgcct ccacacgctg ggtttcactg gcctgcaact gctcgccggt gtgttgcaca
    26521 gccgcggcca tgcttaccag ttcgtcgatc tggctagtcg gtgcggcggg ctgataatta
    26581 ccctggccca gctcgcgcat catcccgaca atcccatcaa tcggctgtac cagctgctca
    26641 ctcaggcgcc gcgaacgcgc ccacatcaag gcaaaaaaca gcaggtagaa cagcgccagg
    26701 ccgacgatca gcagataacc gatatgcagg tagcgctcgg ccaggcgatt ggtctcgctg
    26761 aagatatgct cctcatccac taccagcagt agttgccagc cggtctgcgg aatctcgctc
    26821 caggcgatca gctgattgcg cccgcccagc accacttcct gcacattgcc ctggccagtg
    26881 gtcatggcct gcaacaacgg ctgcatctcg gcgcgtttgg ccaggctgaa gtcttcgggc
    26941 ttgagtacct cacggcgcac cgcctcggca taggagtatt ccgtcagttc gcgcaggccg
    27001 aagtcctctt cgccagcact cggcaaggcc atgatgttgt gatcgcggct gaccagcatg
    27061 gcatagccct gccagggcac atccaactcg ccaatctcat caagcatctg cccgaccgtg
    27121 atatccagcc ccaccacgcc ttcgaggaaa tcaccgcgat acaccggcgc cactgccgac
    27181 atcatccagc ccatgccggc cgggtcgagg tagacatcgg tccaggccac tttgcgctcg
    27241 gggttgtgct tggcgtccgc caggtaatag aagttgtaat cgggtatcac catgtcatgg
    27301 ggatactgct cgggcgtcat aaagaagggg tagatgcggt tgtaactgtc ccaagtattg
    27361 aaataggccg ccgctacccg agggttggcc gcctggattg aacgcatcaa aggatcgagc
    27421 tgcgacagac gcaacgcctt ggcatggtcc tgctgggcca gcggtgtgct gttggcataa
    27481 aaggaagccg cacgcccatc atcgctgcgg ctgtagaaca cgccattgtc ggtgatggca
    27541 tggcgctgac gctccagcgc atcgggctga aaatccgtgt tggccagggc gttcgccgcc
    27601 gcatcacgga agatctgtat ctgggcttcc accgaacgta gacggctatc aatcacctga
    27661 ccttcacgcg ctaccgccgc cgatagatca tccagtgcgc tctgctgcaa atggtcaatc
    27721 tgtgcatcgc gaatcgccgc gttgctcagc agatacaccg taatcaacac cgattcgacc
    27781 agaatcagcg gaatcagggc actctgcaca aaagcgcgcc agatccactg acgcaaaggg
    27841 taacgcgacg cgcgcggcat acgagggtct catccagaag acaaagcgaa tgtgcaggat
    27901 gcaatcatag tcggcaagcc ccttgagcgt ccgcaaggca attgcctggt
//
LOCUS       DASRUM010000011         6472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_838524, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000011 DASRUM010000000
VERSION     DASRUM010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838524"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /gene="serC"
                     /locus_tag="VFF22_00750"
     CDS             <1..1028
                     /gene="serC"
                     /locus_tag="VFF22_00750"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HZX15385.1"
                     /translation="QRAQAELLDWQGKGLSVMEMSHRSDEYTAIAEKAEQDLRDLMEI
                     PSDYKVLFLQGGASQQFAEIPLNLLPEDGVADYIDTGIWSKKSIEEASRYGNINVAAS
                     AKAYDYFAIPGQNEWQLSKDAAYLHYASNETIGGLQFDWIPEVGDVPLVVDMSSDILS
                     RPIDVSKFGLIYAGAQKNIGPSGLVVTIVREDLLGRARSVCPTMLNYKIAADNGSMYN
                     TPATFSWYLSGLVFEWLKEQGGVAAMERINRAKKDLLYKAIDTSDFYSNPIAHNARSW
                     MNVPFRLADEKLDKPFLAGADARGLLNLKGHRSVGGMRASIYNAVGLDAVEALVAYMA
                     EFEKEHG"
     gene            1028..2134
                     /gene="pheA"
                     /locus_tag="VFF22_00755"
     CDS             1028..2134
                     /gene="pheA"
                     /locus_tag="VFF22_00755"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HZX15386.1"
                     /translation="MTDPISEQELQALRLRIDNLDEKILALISDRASCAEEVARVKMK
                     ALPEGEKPVFYRPEREAQVLKRIMERNQGPLGNEEMARLFREIMSSCLALENPLKVAY
                     LGPEGTFSQAAAMKHFGHAVISVPMAAIDEVFREVAAGAVNFGVVPVENSTEGAINHT
                     LDSFLEHDMVICGEVELRIHHHLLVGEATKTDKITRIYSHAQSLAQCRKWLDAHYPNV
                     ERVAVASNADAAKRVKSEWNSAAIAGDMAASLYGLAKLAEKIEDRPDNSTRFLIIGSQ
                     EVPPTGDDKTSVIISMRNKPGALHELLVPFHNNGIDLTRIETRPSRSGKWTYVFFIDF
                     VGHHRDPLIKDVLEKINQEAVALKVLGSYPKAVL"
     gene            2226..3332
                     /gene="hisC"
                     /locus_tag="VFF22_00760"
     CDS             2226..3332
                     /gene="hisC"
                     /locus_tag="VFF22_00760"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZX15387.1"
                     /translation="MTDFLALALPGVQQLSPYVPGKPVDELARELDLDPASIVKLASN
                     ENPLGPSPKALEAIRGELAELTRYPDGNGFELKSRLAARCGVQISQVTLGNGSNDILD
                     LVARAYLAPGLNAVFSQYAFAVYPISTQAVGAQGKVVPAKDYGHDLEAMLAAIDENTR
                     VVFIANPNNPTGTWFGPDALERFLARVPGNVLVVLDEAYIEFAEGDELPDGLKYLARY
                     DNLLVSRTFSKAYGLAALRVGYALSSAQIADVLNRVRQPFNVNSLALAAACAALDDAD
                     YLTQSRQLNDAGMAQLEAGLRELGLSWIPSKGNFIAVDFGRDTAAINQALLRAGVIVR
                     PVAGYGMPNFLRVSIGLPAENARFLDALAKVLNA"
     gene            3329..5584
                     /locus_tag="VFF22_00765"
     CDS             3329..5584
                     /locus_tag="VFF22_00765"
                     /EC_number="1.3.1.12"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019650497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional prephenate
                     dehydrogenase/3-phosphoshikimate
                     1-carboxyvinyltransferase"
                     /protein_id="HZX15388.1"
                     /translation="MSEANSAVTQVQPAAPKLGRLVVIGLGLIGGSFAKGLRERGLCR
                     EVIGVDLDPESRRLAVELGVVDRCEVDLAAACQGADIIQLAVPILAMEKLLTELAKLD
                     LGTTVLTDVGSAKGNVVRAARLAFTGKAVRFVPGHPIAGSEQSGVEASNAQLFRRHKV
                     ILTPCEQSDDAALALVDALWRELGADVEHMEVEHHDQVLAATSHLPHLLAFTLVDSLA
                     KRSENLEIFRYAAGGFRDFTRIAGSDPVMWHDIFLANREAVLRTLDVFRDDLDALRDA
                     VDAGDGHQLLGVFTRARVAREHFGKILARRAYVDAMHSNDLVFLANPGGKLSGRIRVP
                     GDKSISHRSIMLGSLAVGTTQVEGFLEGEDALATIQAFRDMGVVIEGPHHGRVTVHGV
                     GLHGLKAPAGPLYMGNSGTSMRLLSGLLAAQPFNTTLTGDASLSKRPMNRVAKPLREM
                     GAVIETGPEGRPPLNIKGGQRLTGMDYEMPMASAQVKSCLLLAGLYAAGTTSVTEPAP
                     TRDHTERMLRGFGYPVTVDGSTASVTSGHKLTATHIEVPADISSAAFFLVAGSIAEDS
                     ELVLEHVGINPTRTGVIDILKLMGGDITLENQREVGGELVADIRVRSARLKGIDIPED
                     LVPLAIDEFPVLFVAASVAEGRTVLRGAEELRVKESDRIQVMADGLIALGVKAEPTPD
                     GIIIEGGSIGGGEVWAHGDHRIAMSFSVASLRATAPIRIHDCANVATSFPNFLALSAQ
                     VGIRVAEEGKA"
     gene            5581..6267
                     /gene="cmk"
                     /locus_tag="VFF22_00770"
     CDS             5581..6267
                     /gene="cmk"
                     /locus_tag="VFF22_00770"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HZX15389.1"
                     /translation="MIVAPVITIDGPSGSGKGTIAGLLAKQLGWNLLDSGALYRLLAF
                     AAGNHGVDLTNEEAMKLLAAHLDVQFIAAGGGHGQRIILEGEEVTDLIRNEQVGAGAS
                     QVASLSAVREALLQRQQAFQEMPGLVADGRDMGTVVFPDAPLKIFLTASAEERARRRY
                     LQLKAKGDDVNLASLLDEIQARDERDTQRAVAPLKPAADAIQLDSTELSIEQVLERIL
                     SEVANRDLAG"
BASE COUNT         1244 a         1946 c         1994 g         1288 t
ORIGIN      
        1 tgcaacgcgc ccaggcggag cttcttgatt ggcaaggcaa aggcctgtcg gtcatggaaa
       61 tgagtcaccg cagcgacgaa tacaccgcca tcgcggaaaa ggccgagcag gacctgcgcg
      121 acctgatgga aattccgtcc gactacaagg tgctgttcct gcagggcggg gccagccagc
      181 agttcgctga aatcccgctc aacctgctgc cggaagatgg cgttgccgac tacatcgaca
      241 ccggtatctg gtcgaagaaa agcatcgaag aagccagccg ctacggcaat atcaatgttg
      301 ctgccagcgc caaggcctat gactatttcg ccatccccgg gcagaacgag tggcagctgt
      361 cgaaagacgc cgcctacctg cactacgcca gcaatgaaac catcggcggc ctgcagttcg
      421 actggattcc cgaagtgggc gacgtgcctc tggtggtgga tatgtcttcc gatatccttt
      481 cgcgccctat agatgtgtcg aagttcggcc tgatctacgc cggcgcgcag aagaacatcg
      541 gtcccagcgg cctggtggtg accatcgttc gtgaagacct gctcggccgt gcccgcagcg
      601 tctgtccgac catgctcaac tacaaaatcg ccgccgataa cggctcgatg tacaacaccc
      661 cagcgacctt ctcctggtac ctgtccggcc tggtgttcga gtggctgaaa gagcagggcg
      721 gggtggccgc catggagcgg atcaaccgcg ccaagaaaga cctgctgtac aaggccatcg
      781 ataccagcga tttctacagc aacccgattg cccataacgc ccgctcctgg atgaacgtgc
      841 cgttccgcct ggccgacgag aagctggata agccgttcct cgccggtgcc gatgcgcgcg
      901 gcctgctcaa cctcaagggc caccgttcgg tcggcggcat gcgtgcttcc atctataacg
      961 ccgtgggcct ggacgccgtt gaggcattgg tggcctatat ggccgagttc gagaaggagc
     1021 acggctgatg accgatccaa tttccgagca ggaactgcag gccctgcgcc tgcgcatcga
     1081 caacctcgat gagaagattc tcgcgctgat cagtgatcgc gccagctgcg ccgaagaagt
     1141 cgcgcgggtg aagatgaagg ccttgccgga aggcgagaag ccggtgttct atcgccctga
     1201 gcgcgaagcc caggtgctca aacgcattat ggagcgcaac caggggccgc tgggcaacga
     1261 ggaaatggcg cggctgttcc gcgaaatcat gtcctcctgc ctggccctgg aaaatccgct
     1321 caaggtcgct tacctcggcc cagagggtac tttctcccag gccgcggcga tgaagcactt
     1381 cggtcacgcg gtgatcagcg tgccgatggc ggctatcgat gaagtgttcc gcgaagtggc
     1441 cgccggtgcg gtgaacttcg gcgtggtgcc ggtggaaaac tccaccgaag gcgcgatcaa
     1501 ccacaccctc gacagcttcc tagaacacga catggtcatc tgtggtgaag tcgaactgcg
     1561 cattcatcat cacctgctgg tcggtgaagc caccaagacc gacaagatca cccgcatcta
     1621 ttcccacgcc cagtccctgg cgcaatgccg caagtggctg gacgcgcatt acccgaatgt
     1681 tgagcgtgtg gcggtggcga gcaacgccga tgcagccaaa cgggtgaaaa gcgagtggaa
     1741 cagcgcggcg attgccggcg atatggcagc cagtctgtac ggtctggcca agctggcgga
     1801 gaagatcgaa gaccgtcctg acaactccac gcgcttcctg atcatcggta gccaggaagt
     1861 gccgccgact ggcgacgaca agacctcggt catcatctcc atgcgcaaca agccgggcgc
     1921 gctgcatgaa ttgctggtgc cgttccacaa caacggtatt gatctgacgc gcatcgaaac
     1981 ccgcccgtcg cgcagcggca agtggaccta cgtgttcttt atcgactttg ttggtcacca
     2041 ccgcgacccg ctgatcaagg acgtgctgga aaagatcaat caggaagccg tggcgctcaa
     2101 ggtgctgggt tcctacccca aagcggtact ttaaaactgc tgcggcacgc ctcaggtctc
     2161 aagctacacg cgcatcgacg ccgggctgta cttggggctt gcggctttaa gcgtgaggct
     2221 ttgctatgac cgatttcctc gccctggccc tgccaggcgt acagcagctg tcgccctatg
     2281 tgccgggcaa gccggtggat gagctggccc gtgagctgga tctggacccg gccagcatcg
     2341 tcaagctggc cagcaacgag aatccgctgg gtccaagccc caaggcgctg gaagcgattc
     2401 gcggcgagct tgccgagctg acccgttacc ccgatggcaa tggcttcgag ctgaaaagcc
     2461 gtctggccgc gcgctgtggc gtgcagatca gccaggttac cctgggcaat ggctccaacg
     2521 atattctcga tctggtcgcc cgcgcctatc tggcccccgg ccttaatgcg gtgtttagcc
     2581 agtatgcctt tgccgtttac cccatctcga cccaggccgt aggtgctcag ggcaaagtgg
     2641 tgccggccaa ggactacggt cacgacctgg aagccatgct tgcggccatc gatgaaaaca
     2701 ctcgcgtggt gtttatcgcc aacccgaaca acccgaccgg cacctggttt ggccccgacg
     2761 cgctggagcg ctttctcgct cgcgtacccg gcaacgtgct ggtggtgctg gatgaagctt
     2821 atatcgagtt cgccgaaggt gatgagctgc cggacggcct caagtacctg gcgcgctatg
     2881 acaacctgct ggtctcgcgc actttctcca aggcctatgg cctggcggcg ctgcgcgttg
     2941 gttatgcgct cagttcggcg cagatcgccg atgtgctcaa ccgtgtacgc cagccattca
     3001 acgtcaacag cctggccctg gccgccgcct gcgcggcgct ggatgatgcc gattacctga
     3061 cgcaaagccg tcagctcaat gacgccggca tggcgcagct ggaagcaggc ttgcgcgaac
     3121 tgggcctgag ctggatcccg tcgaaaggca actttattgc cgtggacttt ggccgtgata
     3181 ccgcagcgat caaccaggcg ctgctacgtg ctggggtgat cgtgcggccg gtagccggtt
     3241 acggcatgcc gaacttcctg cgcgtttcga ttggtctgcc tgctgagaat gcgcgcttcc
     3301 tcgacgccct ggccaaggtg ctgaacgcgt gagtgaagcc aactctgctg tcactcaggt
     3361 gcaacctgcc gcccctaagc tcggccgcct ggtggttatt ggcctgggcc tgattggtgg
     3421 ctctttcgcc aagggtttgc gtgagcgcgg cctgtgccgc gaagtgatag gtgtcgatct
     3481 cgatcccgag tcgcgccggt tggcggttga gctgggtgtg gtcgatcgct gtgaggtcga
     3541 tctggctgcc gcctgtcagg gtgccgatat tatccagctg gcggtgccta tcctggccat
     3601 ggaaaagctg ctcacagagc tggctaaact tgacctgggt acgacggtgc tcaccgacgt
     3661 gggcagcgcc aaaggcaatg tggtgcgcgc ggcgcgactg gcctttaccg gcaaggcagt
     3721 gcgctttgtg cctgggcatc cgattgccgg ttccgagcag agcggggtgg aagcgtccaa
     3781 tgcgcaactg ttccgccgcc acaaggtcat cctgacgccc tgcgaacaga gtgatgacgc
     3841 agctctggcg ctggtcgacg ccctatggcg cgagctgggc gcggatgttg agcatatgga
     3901 ggtcgagcat cacgatcagg tgctggctgc gaccagtcac ctgccgcatt tgctggcctt
     3961 taccctggtt gactcgctgg ccaagcgcag tgagaacctg gagatctttc gttacgccgc
     4021 cggcggcttt cgcgacttca cgcgaattgc cggcagtgat ccggttatgt ggcacgacat
     4081 cttcctcgcc aatcgcgagg cggtgttgcg cacactagac gtatttcgcg acgacctcga
     4141 cgccttgcgc gacgcggtcg acgctgggga tgggcatcaa ttgctgggcg tattcactcg
     4201 cgcccgcgtg gcccgtgagc attttggcaa aattctggcc cgcagggcct atgtggacgc
     4261 tatgcactcg aatgatctgg tttttctggc taacccaggc ggcaaactct ccgggcgtat
     4321 tcgcgtaccg ggcgacaaat ccatctccca ccgttcgatc atgcttggct cgctggctgt
     4381 cggcaccacg caggttgaag gcttcctgga aggtgaagac gccctggcga ccattcaggc
     4441 gttccgcgat atgggcgtgg tcatcgaagg cccacatcac ggccgcgtga ccgtgcatgg
     4501 cgttggcctg catggcctga aggctcctgc tggcccgctg tatatgggca actccggcac
     4561 ctcgatgcgt ctgctgtccg gcctgctggc tgcacaacct ttcaatacaa ccctaacggg
     4621 cgatgcttcg ctatccaagc gcccgatgaa ccgcgtcgct aagccgctgc gcgagatggg
     4681 tgcagtgatc gagaccggtc ctgaaggccg tccgccgctg aatatcaaag gcggccagcg
     4741 cctgaccggc atggactacg aaatgcccat ggccagtgcc caggttaaat cctgcctgtt
     4801 gctggcgggg ctatacgccg ccggaacaac ctcggtgacc gagcctgcgc cgacccgtga
     4861 ccacacggaa cgcatgctgc gtggctttgg ctacccggta accgtggatg gcagcaccgc
     4921 cagcgtcacg tcgggccaca agctgactgc tacccatatc gaagtgccgg cggatatctc
     4981 ctctgcagcc tttttcctgg tggccggcag cattgccgaa gactccgagc tggtgctgga
     5041 acacgttggg atcaacccaa cccgtacggg cgtgatcgac atcctcaagc tgatgggcgg
     5101 cgatatcacc ctggaaaacc agcgtgaagt cggtggcgag ctggtggcgg atatccgcgt
     5161 gcgcagtgcc aggctcaagg gcatcgatat ccccgaagac ctggtgcccc tagctattga
     5221 tgagttcccg gtgctgttcg tcgccgcttc ggtggccgaa ggtcgcaccg tgctgcgtgg
     5281 cgccgaagag ctgcgggtca aggaatccga ccgtatccag gtgatggctg atggcttgat
     5341 tgctctgggc gtcaaggctg aaccaacgcc agacggcatc attatcgaag gcggcagcat
     5401 cggtggtggc gaagtctggg cgcacggcga tcaccgtatt gctatgtcgt tcagcgtggc
     5461 atcgctacgc gctactgcgc cgatccgcat ccatgattgc gccaacgtcg ccacctcgtt
     5521 ccccaacttc ctcgcgctgt cggctcaggt tggcattcgt gtggccgagg agggcaaggc
     5581 atgatcgtcg caccggttat caccatcgac gggccgagcg gctcgggcaa aggcaccatt
     5641 gctggcctgc tggccaagca actgggctgg aatctactgg attcaggggc gttgtatcgt
     5701 ctgctggcct ttgctgcagg caatcacggc gttgatttga ccaatgaaga ggcaatgaag
     5761 ctgctcgccg ctcatctgga tgtgcaattt atcgccgccg gtggcggtca tggccagcgc
     5821 atcattcttg aaggcgaaga agtcacagac ctgatccgca atgagcaggt cggcgccggt
     5881 gcatcgcagg tggcatcgct gtctgccgtg cgtgaagctc tgctgcagcg tcagcaggct
     5941 ttccaggaaa tgccgggcct ggtcgcagac ggtcgcgaca tgggtacggt ggtatttccc
     6001 gatgcgccgc tgaagatttt cctcaccgcc agcgccgagg aacgtgcccg tcggcgctac
     6061 ttgcagttga aggccaaggg cgatgatgtt aatctcgcga gtcttcttga tgagattcag
     6121 gcgcgcgatg agcgcgatac ccagcgtgca gtggctccgc tcaagccggc agccgatgcg
     6181 atccagctgg attccactga actctcgatt gaacaagtgc ttgaacgaat tctgagtgaa
     6241 gtcgccaatc gcgaccttgc cggatgataa gagccacagc tgcttaagct gaccggctcg
     6301 caaggaggcg cacgggaacc cagtcctagt tccgtttgcc tctttttaaa tgaacgtacc
     6361 cacattgtct ggaatgtggt ttgggcggat tccctgccct gaatcaacag gaattaaaat
     6421 gagcgaaagc tttgctgaac tgtttgaaga aagcctaaaa accctgaatc tt
//
LOCUS       DASRUM010000012         6999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_674198, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000012 DASRUM010000000
VERSION     DASRUM010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674198"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1507)
                     /locus_tag="VFF22_00775"
     CDS             complement(<1..1507)
                     /locus_tag="VFF22_00775"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZX15390.1"
                     /translation="MLARALRPMAHLSLAAVLTAVVALTGCQSLLNSRYSDSVHPDQG
                     IVRVKGLAQSVVIRRNPLGMPLIETTTFHDALFALGYVHASDRLSQMVGLRLMAEGRL
                     AEMAGPGVLEMDRFMRAVNLKKSAEVLYSDASPRIKRFFEVYARGVNAYLFRYKDKLP
                     MDLAESGYRPAYWKAEDSVLVFCLLNFGLAVNLQEEIASLVLAQKVGSEQLAWLLPTY
                     PDEPLPVEEAAKLKGLNLNGQLAGLSDISAAVSQFADAHMLGVAASNNWAFAPQRTRS
                     GKSLLANDTHLPLSMPSVWNFVQIRSPKYQAAGVSIAGVPAVVAGFNGKLGWGMTMVM
                     GDNQDLFLEQVKRQGGRLHYLADGKWLPAQQRQETFFIKGQRPIRETIFETRHGPLLN
                     SVLGERKHMLQPMQLSSGYGLALKTTQFEADKTLDAFFDLSRAQSVEQAFEATREVRA
                     MPLNIVFADAQHIGWQVTGRFPNRRAGLGLMPSPGWDSQYEWDGFADPMLHP"
     gene            complement(1636..2100)
                     /locus_tag="VFF22_00780"
     CDS             complement(1636..2100)
                     /locus_tag="VFF22_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2489 domain-containing protein"
                     /protein_id="HZX15391.1"
                     /translation="MNNLTLLVLIGSAVLIAALALYALHLWRRVWTQEKAQTEALNAQ
                     RELLGGDLHILASSLLEGQLPLIEGAIRIKVLLDNYDSRLSQDQRCEVFHLLFAATAQ
                     VPTHAEWKALERSVRRQHEQHFSELELQHKAAARAAARWLLDEALPPLRQSA"
     gene            complement(2108..2311)
                     /locus_tag="VFF22_00785"
     CDS             complement(2108..2311)
                     /locus_tag="VFF22_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HZX15392.1"
                     /translation="MTQQEHVHGPDCNHDHDHAHPHDHGHVHGPHCNHGHQEPARNAL
                     KDVGRNDPCPCGSEKKFKKCHGA"
     gene            complement(2317..2808)
                     /locus_tag="VFF22_00790"
     CDS             complement(2317..2808)
                     /locus_tag="VFF22_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016851905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HZX15393.1"
                     /translation="MFYQAQTIRILSLLMFFGLLSGLSGCSSRGDFKSPEVRLVKVDV
                     VKAKLLEQHFTLRFRIDNPNNFSLPVRGMDYLVHLNQVKLAEGESNVSFTVPAHGHHN
                     FSIPVRTNLWRHLRQIVKALEKPDRPIPYRLQGAVKTGWFFGQSVHMSRNGEIIPGDY
                     IPE"
     gene            2921..3484
                     /locus_tag="VFF22_00795"
     CDS             2921..3484
                     /locus_tag="VFF22_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003086339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15394.1"
                     /translation="MARYEIAFSAQLVPGAQLALVKANLAKLFQADEQRLALLFSGRR
                     IVIKSNLDAAGAEKYRSTLERAGAVVEVACLDDEIEEIELAAPPAVAPAPVAAPVRPA
                     ATSKPAGRLQVAPRDEYMAAFAEVDAPDFGIAPLGDDVQDGKPQVQAPVLDLSQFSLA
                     PAGSDMGEAKKAPAGPPPDISHLKLQD"
     gene            complement(3545..4015)
                     /locus_tag="VFF22_00800"
     CDS             complement(3545..4015)
                     /locus_tag="VFF22_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011332777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HZX15395.1"
                     /translation="MSSTNCPCGSGQLLTLCCGRYHAGAQPPSAEALMRSRYSAYALG
                     VIDYLVHSTLPAQQAGLDHEAIRTWSLGSTWLGLEVEETQLVEGEPQHAHVTFTAHWR
                     DESGAHSHRERSAFVCNNGQWYFIDPTVELKVGRNDACPCASGLKFKKCCASYF"
     gene            complement(4020..4517)
                     /locus_tag="VFF22_00805"
     CDS             complement(4020..4517)
                     /locus_tag="VFF22_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6231 family protein"
                     /protein_id="HZX15396.1"
                     /translation="MPATPSTRTPQQALAALLARYTPERLLVVGSRKLPAIDAFIQTH
                     SSCHLVHADAGALPGEVAAQRFDLALIVDCLEHLPKRAGLELLGGIRNLNASRMAVMV
                     DLQACDWQETDFFALALQASECFQRDEQTLNLFTYDLLDYKQVPDWLNAKFWANPENF
                     GKYWW"
     gene            4958..5311
                     /locus_tag="VFF22_00810"
                     /pseudo
     CDS             4958..5311
                     /locus_tag="VFF22_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250468.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
     gene            5453..6175
                     /locus_tag="VFF22_00815"
     CDS             5453..6175
                     /locus_tag="VFF22_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014336872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZX15397.1"
                     /translation="MMGIAPILLVSSVLSGCVTTSSTGDAPLNQGNWPLCSVIGGLTG
                     AGLGAIESSTWAAGGAVAGAVIGTLICYAQDGDEDDDGVFDRRDRCPGTPPNTPVHHN
                     GCPIKEYANTPVDAEPESIEVVDSVRVELDVKFDFNKSDVKEDSQADIKSLADFMKQY
                     PQTTTTVEGHTDAVGSDAYNQGLSERRANAVRDVLVNQHGIDSGRVSSVGYGESQPVA
                     DNESESGRAINRRVEAEVEAQP"
     gene            6366..6668
                     /locus_tag="VFF22_00820"
     CDS             6366..6668
                     /locus_tag="VFF22_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003082018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 domain-containing protein"
                     /protein_id="HZX15398.1"
                     /translation="MKTFLTLGKVLTALFWGVLLANLLQPFAQPFALLLNGAGAFILL
                     IHALELWFFDKRIAACPKPAHERVHVMLFGVFQLLGLPAEPVAEPEPALQMEAENA"
     gene            6661..>6999
                     /locus_tag="VFF22_00825"
     CDS             6661..>6999
                     /locus_tag="VFF22_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HZX15399.1"
                     /translation="MRKLIVLAALFSPLALAQAVIEVDSHAVMRLPSTTSVLLLDRLA
                     IADNGTLLIPSGLTEIRVAQLQLGRDARLAIAPGDQVLRLEIETADIASGAQISARGA
                     RGSAEKPALPG"
BASE COUNT         1409 a         1872 c         2193 g         1525 t
ORIGIN      
        1 agggatgcag catcgggtcg gcgaatccgt cccactcata ctgactgtcc cagccgggtg
       61 aaggcatcag gcccaggccg gcgcggcgat tggggaaacg tccggtgact tgccagccga
      121 tatgctgtgc atcggcaaat acgatgttca gcggcatggc gcggacttca cgggtcgcct
      181 caaaggcctg ctcgaccgac tgggcgcgcg agagatcgaa gaaggcatcc agggttttat
      241 ccgcctcgaa ctgggtggtt ttcagcgcca ggccatagcc gctgctcagc tgcatgggtt
      301 gcagcatgtg cttgcgttcg cccagcaccg agttcaatag cgggccgtgg cgggtttcga
      361 aaatggtttc gcggattggc cgctgacctt tgataaagaa ggtctcctgg cgttgctgtg
      421 ccggtagcca tttgccgtcg gccaggtagt gcaggcgtcc gccttggcgt tttacctgct
      481 cgaggaacag atcctggttg tcgcccatga ccatggtcat gccccagccc agtttaccgt
      541 tgaaaccggc caccaccgcc ggtacgccag caatcgatac gccggctgcc tggtatttgg
      601 gtgagcggat ttgcacgaaa ttccacaccg agggcatcga cagcggcagg tgggtatcgt
      661 tggccagcag gcttttgcca ctgcgtgtgc gctgcggggc aaaggcccag ttgttggagg
      721 cggccacgcc gagcatatgc gcatctgcaa attggctgac ggcggcgctg atgtcgctca
      781 gaccggccaa ttggccattg agattgagcc ccttgagttt cgcggcttct tcaacaggca
      841 agggctcgtc tgggtaggtt ggcagtagcc aggccagctg ctcgctgccg actttctgtg
      901 cgagcaccag tgaggcaatt tcttcctgca gattgaccgc caggccgaaa ttcagcaggc
      961 agaacaccag caccgagtcc tcggccttcc agtacgccgg gcgatagccc gactcggcca
     1021 ggtccatcgg cagcttgtct ttgtagcgga acaggtaggc gttgactccg cgcgcataga
     1081 cctcgaagaa gcgtttgatc cgcggcgagg cgtcgctgta gagcacctcg gcgcttttct
     1141 tcaggttgac cgcgcgcata aagcggtcca tttccagcac gccagggccg gccatttcag
     1201 ccaggcggcc ttcggccatc aggcgcagac cgaccatctg actgaggcgg tcgctggcat
     1261 gcacatagcc cagggcgaac agcgcgtcat ggaaggtggt ggtttcgatc agcggcatgc
     1321 ccagcgggtt acggcgaatc accacgcttt gcgccaggcc cttgacccgg acgatgccct
     1381 ggtctgggtg cacgctatcg ctgtaacggc tgttcagcaa tgactggcag ccagtgagcg
     1441 cgacgacggc cgtaagaacg gcggcgaggc tgagatgggc catggggcgt aacgcgcgcg
     1501 ctagcatgca gcaggctcct tgcgggtgga aaacggtcgg ttggggtgtg cactttccag
     1561 atgcgtgggc gcgcaagtgc gaacgtacta cgttagccag cagccctgcg cctgacaagg
     1621 cgcagggcga gggagtcagg cgctttgccg caggggcggc agggcttcat caagcagcca
     1681 gcgggcggcg gcgcgagcgg cggccttgtg ctgcagctcc agttcgctga agtgttgctc
     1741 atgctggcgg cgcacgcttc tctccagggc cttccactcg gcgtgggtcg gcacttgggc
     1801 ggttgccgcg aacaacaggt gaaaaacctc acagcgctgg tcctggctga ggcggctgtc
     1861 gtagttatcc agcagcacct tgatccgaat ggcgccctca atcagcggca actgaccttc
     1921 gagcaagctg ctggcgagaa tatgcagatc gccgccgagg agttcgcgct gcgcattgag
     1981 tgcctcggtc tgggcttttt cctgggtcca gacgcgtcgc cacaggtgca gggcatagag
     2041 tgccaatgcg gcaatcaata cggcgctgcc gatcaggacg agcagggtga ggttattcat
     2101 cgcaggttta tgcgccgtgg catttcttga attttttctc gctgccgcac gggcaggggt
     2161 cgttgcggcc cacatccttc agggcgttgc gcgcaggctc ctgatgacca tgattgcaat
     2221 gcgggccatg cacgtggcca tgatcatggg ggtgagcgtg gtcgtggtca tggttgcagt
     2281 cagggccgtg gacgtgttct tgctgggtca tgcgagttac tccgggatgt aatcgccggg
     2341 aattatctcg ccattgcgcg acatgtgcac gctctgtccg aaaaaccagc cagtttttac
     2401 ggcaccttgc aggcggtagg ggatggggcg gtcgggtttt tccaaggctt tgacgatctg
     2461 ccgcaggtga cgccagaggt tggtgcgcac tggtatggag aagttgtgat gaccgtgggc
     2521 gggtacggtg aacgacacgt tggattcgcc ttcggccagt ttgacttggt tcagatgcac
     2581 caaatagtcc atcccgcgca ctggcaggct gaagttgttg gggttatcga tgcgaaagcg
     2641 cagcgtgaaa tgctgctcca acagtttggc tttaacgacg tcgactttaa cgaggcgtac
     2701 ttcgggcgac ttgaaatcgc ctcgtgagga acaaccgcta aggcccgaca ggaggccaaa
     2761 aaacatcaat aggctgagaa ttcttatcgt ttgcgcctga taaaacattg catactccga
     2821 atgacgcatc agtgtaacaa gcaagtgctt ggctgcaacc tgttgtgacg gtaaaaaaac
     2881 ctgcgccata ctgagcaacg aataggacag gagtatgacc atggctcgct acgaaattgc
     2941 ctttagtgcg cagttggtcc cgggtgccca gttggcgttg gtgaaagcca acctggccaa
     3001 gttgttccag gcagatgaac agcgactggc gctgttgttc tcggggcggc gtatcgtgat
     3061 caaaagcaat ctggatgcgg ccggtgcgga aaaataccgc tcgaccctgg agcgcgctgg
     3121 cgctgtggtc gaagtggcct gcctggatga tgagatcgaa gaaattgagt tggccgcgcc
     3181 gcctgctgtg gctccggctc cagttgctgc gcctgttcgg ccagcggcta ccagcaaacc
     3241 ggcaggccgc ttacaggtag cgccgcggga tgaatatatg gcggcgttcg ctgaggtgga
     3301 tgcaccggat tttggcattg cgccgctggg tgatgatgta caggacggca agccgcaagt
     3361 gcaggctcca gtcctggatt tgtcgcagtt cagtctggcg ccagccggca gcgacatggg
     3421 cgaagcgaaa aaagcgccag ccgggcctcc gccggatatc tcccatctga aattgcagga
     3481 ttagtctgca ctgcgcagag agcgccttac cggtgctttc tgcgctatca acccgcttca
     3541 gcagtcaaaa ataactggcg cagcacttct tgaatttcag cccactggcg caagggcagg
     3601 cgtcattgcg accgaccttc aactcgacag tagggtcaat aaaataccac tggccgttat
     3661 tgcacacgaa tgccgagcgt tcacggtggc tgtgtgcgcc gctttcatca cgccagtgcg
     3721 cggtaaaggt gacatgggcg tgctggggtt cgccctcgac cagttgcgtc tcttcaacct
     3781 caagcccgag ccaggtactg cccaagctcc acgtgcggat agcctcgtgg tcgaggcctg
     3841 cttgttgggc cggtagtgtg ctgtgcacca ggtaatcaat cactcccagg gcataggcgc
     3901 tgtagcgtga gcgcatcaaa gcttcggcgc tgggtggctg agcgcctgcg tgataacggc
     3961 cgcagcaaag tgtcagtagt tggccgctgc cgcaggggca gtttgtgctg ctcatggctt
     4021 taccaccaat acttgccaaa gttttccggg ttggcccaga acttggcatt cagccagtct
     4081 ggtacttgct tgtagtcgag caagtcgtaa gtgaatagat tgagtgtctg ttcatcgcgc
     4141 tggaagcact cactggcctg cagggccagg gcaaaaaagt cggtttcttg ccagtcacag
     4201 gcctgcaagt cgaccattac ggccatacgg ctggcattca ggttacgaat gccgccgagc
     4261 agctctagtc cggcgcgttt cggcaagtgc tcaaggcaat cgacgatcag cgccagatcg
     4321 aaacgttgcg cggctacttc acccggtagc gctccggcat ccgcatgaac gaggtggcag
     4381 cttgaatggg tctggataaa tgcatcaatc gcaggtaatt ttcggctacc gactaccaac
     4441 aggcgttctg gggtgtaacg cgccaacaga gcggcaaggg cttgctgggg tgttcgggtg
     4501 gacggggttg caggcatgac ggctcctcgc tcaagtgcgc aaagactaac gtgcagtagc
     4561 aacgcggcct agtgcggatt gtgctagtaa tgtgtttgca tattctctgg catattttgt
     4621 tactgacgtc tttacttcgt cagtatcggt ttttgccgat ttaccaatag gagacgtata
     4681 tatgagcatc accaggaaag cattacccct gattgtcgcg agtacctttt tgaccggttg
     4741 tgccgggctg cagaaaacgg attggccaac ctgtgccgcg atcggcggtg taggcggtgc
     4801 tgctctgggc gctattgaaa gctcttcatg ggctggtggc ggtgcagtag ttggtgccgg
     4861 tatggcagct gcatattgct gggtgcatgg cgccagcgat gagcaggttg ttatggttga
     4921 agaagtggct gtggaagaag tcgcggtggc tgaacccgcc gaagcggtac gtgtagagct
     4981 ggacgttaag ttcgactttg acaaagccca agtcaaacca gaaagctatg gtgatatcaa
     5041 aaacctggct gacttcctga cccaataccc acaaaccacc accgtggttg aaggtcatac
     5101 cgactccgtt ggtagcgacg cttacaacca gactctgtct gagcgtcgtg ccaatgcggt
     5161 acgtgatgta ctggttaacc agtacggcgt aggcagtgat cgcgttaacg ctgtgggcta
     5221 tggcgaatct cgtccggttg cggacaacgc cagtgctgac ggccgtgccg tcaaccgccg
     5281 cgtagaagca gaagtagaag ctcgtccttg atcgtctgat tccagacttt ctgaagaaaa
     5341 caagccagac ccctcggggt ctggcttgtg cagctcgaag gggaggtctt tactcctaag
     5401 ttaccggcag tagccggtac tacaggagat ttctaccatg agagttagat ccatgatggg
     5461 gatagccccc attctcttgg tcagtagtgt tctttcaggt tgtgtgacaa cttccagtac
     5521 gggagatgcg ccgctcaacc aagggaactg gcccctgtgc agtgtgattg gcggtctgac
     5581 cggtgctggt ctgggcgcaa tcgaaagttc tacgtgggca gcaggtggag ccgtggccgg
     5641 tgcggtcatt ggtacgctga tttgttacgc ccaagatggt gatgaagacg atgacggtgt
     5701 gtttgaccgt cgtgaccgtt gtccaggaac ccctcccaat acgccggtgc atcacaacgg
     5761 ttgtccgatt aaggaatatg ccaatacgcc tgtcgatgca gaaccggagt ctatcgaagt
     5821 tgtggactcg gttcgggtag agctggatgt gaagttcgat ttcaacaagt ccgatgttaa
     5881 agaagacagc caggccgata tcaagagcct tgctgacttt atgaagcagt acccacaaac
     5941 caccacaacc gttgaaggcc ataccgacgc cgtaggcagt gatgcctata accagggatt
     6001 gtccgagcgc cgtgccaatg cggtgcgtga tgtactggtc aatcagcatg gtatcgacag
     6061 cggcagggtt tcctctgtcg gctacggtga gtcgcagcca gtcgccgata atgaaagtga
     6121 gtccggacgg gcgatcaacc gccgggttga agcggaggtc gaggcgcagc cataaccgat
     6181 gtgacgcgct ggcaggccgt tgcgaatcta tttgccgggt tcgcaacggt ctgccagcca
     6241 gaaagtggtt tcaacccagt aaaagctatg aaggtattca gcctccatgg tttttactgg
     6301 tcttctggca gcacactccc aaagcagata aggtgcagcg aaataaaaaa ctgccggggg
     6361 cggatatgaa gacatttcta acgttgggta aggtgctaac ggccttgttc tggggcgttt
     6421 tactggcaaa tctgctgcaa cccttcgcac aacctttcgc gctgctgttg aacggcgccg
     6481 gggcttttat tctgctgatc catgcgcttg aattgtggtt ttttgacaag cgcatcgccg
     6541 cctgccccaa acctgctcac gagcgtgtgc acgtcatgct gttcggcgtg tttcagttgc
     6601 tggggctgcc cgctgagcca gtagccgagc ctgagccagc gctgcagatg gaggccgaaa
     6661 atgcgtaagc tcatcgtcct cgctgcgctg ttcagtccgc tggcattagc ccaggcggtg
     6721 attgaagttg attctcatgc ggttatgcgt ctacccagca ctaccagcgt gttgctgtta
     6781 gaccgtctgg cgattgccga taatggcacc ttgctgattc catccggctt gaccgaaatt
     6841 cgtgtcgcac agctgcaact ggggcgtgac gcgcggctgg cgattgcccc tggtgatcag
     6901 gtgttgcgcc ttgaaattga aaccgccgat attgccagtg gcgcacagat cagcgcgcgt
     6961 ggcgcacgcg gcagtgctga aaagccggcg ctgccggga
//
LOCUS       DASRUM010000013        18795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_6038, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000013 DASRUM010000000
VERSION     DASRUM010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 18795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18795
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6038"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VFF22_00830"
     CDS             <1..334
                     /locus_tag="VFF22_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HZX15400.1"
                     /translation="RASMQDTQFGLIDGWLRSIRDLYYEHREHLAQLPSEEEQVDRLC
                     ELNVIQQVANVSHTTIVQNAWHRGQPLAVHGCIYGIKDGRWKNLNVTVSGMEQLPPQY
                     RLRPLGQR"
     gene            337..1236
                     /locus_tag="VFF22_00835"
     CDS             337..1236
                     /locus_tag="VFF22_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZX15401.1"
                     /translation="MLSSVRIALAGLLLATLCWSGNALVARAFAGEIPPFALSFWRWS
                     LALVLLLPFVAKPLWQHRAALRAAGWRLLVLAAVGIAGYNSLLYAAAQTTAAINITLV
                     NTCLPLMTFIGAGLLLGEWPHRRAWWGMAVAAGGLLVLISKGSWASLSGLAFNKGDLI
                     MLLAVADWALYSLLLRRWSAHLLPIPPLALLGVLMLLGVPLILPFYLYELAQGAHFKL
                     TAENLAAISYTAIFASLLAYLAWNHAIRVLGAAKAALSNYLMPVFTAVLGWLLLGEGL
                     QSYHWLGAALIFVGLLLGTQGRR"
     gene            1496..2458
                     /locus_tag="VFF22_00840"
     CDS             1496..2458
                     /locus_tag="VFF22_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15402.1"
                     /translation="MTERSTRSSGKPSLNEQSQENFADNEGWIAWRNLTTEAMRRFHE
                     QLLANVSRAPGLVGYEAAERARAANGSVAWSWGAAAEIQETINTINSWGTHLHDWCAW
                     NAVVESFETDEDKGQLLHHFVNPLAFFCMHQPSAVSDRLMLLAETLLHQANCLIEPGY
                     VDRLDQDGLRHGQGLRRSDRRKQVIRLGHRWTRFNGFLAALDAINDSAYRKLSRNFRD
                     LSVHSFAPRFMVGQIMRAFRSIEPWQELVEQPGGGSLLVDHPTKKCVSYTMCVLEPFP
                     LRDACSASLSEYQKVVAAMSAFSQLLEEVCDSMDAIPDRLSGQS"
     gene            complement(2700..3344)
                     /locus_tag="VFF22_00845"
     CDS             complement(2700..3344)
                     /locus_tag="VFF22_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZX15403.1"
                     /translation="MIKLKKSAIPDVLKDNAAKWTTAVLEKLKQSIELAKSEKNKYNH
                     PSIKEALVKETNGKCAYCESKILHITYGDIEHITPKKIRPELWFDWDNLTLACDICNT
                     KKSSKEHILDPYENDPADRLLFIGPTIWPAPGDEISKYTIEELALNREALTEKRQEKI
                     EYLMLMLDVIAKTNHPQLVETLKSDFKSELTDEKEFSGLARCLASELTAKGFLT"
     gene            complement(3341..4624)
                     /locus_tag="VFF22_00850"
     CDS             complement(3341..4624)
                     /locus_tag="VFF22_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZX15404.1"
                     /translation="MEGIKFKRIALQGWRQFDNIEIDLHERLTIVTGANGAGKSSILK
                     AFSKHFGLELPFLSTPVLVEGRFVYKTGLFISLTKKISSFFKKENHSTVGKISYTNKT
                     ESSLTVQESDSAQYLLQINGMQGISGIHIDSHQIISNYRQVANIPSSMTSPSAAYDLY
                     HNEINNRYHGGHTGYSPLYRMKEAIISMAVFGEGNSRNQGNPELQLALSGFINVLHKV
                     LPASLGFIDLSVRTPEVVLQTKTGEFLLDAASGGVSTLIDLAWRLHMFSLKNESFVVT
                     MDEPENHLHPSMQRSLMQRLLSAFPKVQFIVATHSPFIISSVKDSYVYVLRYNTEDRV
                     VDGFAPETTKSRVISEKLDTVNKASSANEILREVLGVEATTPEWVSEELSIITKKYKN
                     LEITKETLENIRAELKNLGFSDHYPLALAELMKGK"
     gene            complement(4674..5003)
                     /locus_tag="VFF22_00855"
     CDS             complement(4674..5003)
                     /locus_tag="VFF22_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15405.1"
                     /translation="MRRILLALALASSPALAEESKPVATQVGEALRPAAEAYGKTVNT
                     LVNEFFAGSKSPLGEAARSNLKSQNQRELEANRGVRRTMKECIKPGNVIDDDVKECID
                     GMRVKEW"
     gene            5456..5728
                     /locus_tag="VFF22_00860"
     CDS             5456..5728
                     /locus_tag="VFF22_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003115921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HZX15406.1"
                     /translation="MEQSGVVGLNIEGNPERVTDFRDAPFCSMQVLVDMLGTELISPD
                     VVRGWVETSTLPTIKIGRRRVINLHRIRRDLDRGKTVFCAGDYADE"
     gene            6060..6320
                     /locus_tag="VFF22_00865"
     CDS             6060..6320
                     /locus_tag="VFF22_00865"
                     /inference="COORDINATES: protein motif:HMM:NF036935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5447 family protein"
                     /protein_id="HZX15407.1"
                     /translation="MAKPDPCPSTPCDQCRPASVQRGRILSMGLVAGVWMPLKWEPLI
                     TPALYCAKHKPSDRPPKYWSVVENIGKPVPFVPLHEPFELTG"
     gene            6324..6623
                     /locus_tag="VFF22_00870"
     CDS             6324..6623
                     /locus_tag="VFF22_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15408.1"
                     /translation="MQWVEVITLSLSFMCVGILIGYSFGRVAQARAVKADPFLAYTRA
                     ELKGFTCGMLAALVAIHSKTDPPSRSPPVSVTGQRLNSGQPTESPPVAGAGGETG"
     gene            6910..7269
                     /locus_tag="VFF22_00875"
     CDS             6910..7269
                     /locus_tag="VFF22_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15409.1"
                     /translation="MARSTMEVAFLGTQRFDGENGQKYIKVFYGDEPDGKTEHGLSII
                     GMAAEEDAAEEIFAAGTRFEPLQLVRITFDVARGGQNKGKNLALHIEAVDTKATRAAS
                     PQANAQQQAKPADPAKA"
     gene            7762..8067
                     /locus_tag="VFF22_00880"
     CDS             7762..8067
                     /locus_tag="VFF22_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15410.1"
                     /translation="MYQSKKEVAIRGGQLVQQEGTSVGKALGGKAVALFLTGSAALTA
                     GSANAAITVPPELLEVFTDLATAFGTLVAAGAILFGVIRGTLALFKLARSVFGAAGA"
     gene            8087..9643
                     /locus_tag="VFF22_00885"
     CDS             8087..9643
                     /locus_tag="VFF22_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15411.1"
                     /translation="MRAAIGLRLLLALTLMGWGQLALFKFISFSRYFFVFSLVALPIY
                     SYASSPVAVYVRPGHSTPNWPTAEAACKALYGSTAYVAFQVGCYAPPSGNYLGSVQKI
                     HICTTGTLDPATNQCVVPEPPKDCSSHNGKFIIAAVSGSQAGSAYSTPGTVSIDGARY
                     VSAPDGNTRCKPNFDGSGTAICAARYGGDGSCMPSGHSESDDLTEGTFVDVTDTSTGD
                     EKCLTFGGKELCLSPDNAGCDVYNGNPWCYQTGDNCGEVTEPGTTQSKFVCFPKTARQ
                     CTYANGKMECIETGNPPGSKPPAYITPTSSDHPYNGGNADGNDKNDPKAPGAGDDTQY
                     VGGGGPPTDLSQTNDELRGLGEKLDESTGLLEDIKDLLTGDGDDSGNSDGSAADAEAE
                     GAAVGNQIGDAITTQMEAVDEERNEEVEGVLDELPNQVSQWFGADGSAVGLDFIDSIL
                     PTPVACADYVVPLSLNGYTANLRMPVCALMPYKPILEWVIWCLTAIGAWRIGYSALRQ
                     EDAKAVKGGF"
     gene            9643..10014
                     /locus_tag="VFF22_00890"
     CDS             9643..10014
                     /locus_tag="VFF22_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15412.1"
                     /translation="MGYFLNFFKKLFPTAFSWLAKFLLGLIMPILAPVIQAVAALLRK
                     IGIFLLIIAAIGAAIAVLAAGIDYVVSGIGSLVAPDLVIVGRMFLPSNLSFCISLLVF
                     LKLKSLVFYWVTKLSEKLIHT"
     gene            10023..11126
                     /locus_tag="VFF22_00895"
     CDS             10023..11126
                     /locus_tag="VFF22_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HZX15413.1"
                     /translation="MAVYIVTGKLGAGKTLLCVMRILDYLKQGRRVAVNVDVFMDKLC
                     KRDNKHSRLVRLPDLPTADDLTGLGVGCDVYDEEKFGGIFLDEAGVWLNSRDWNSGGR
                     TDLLKFFLFLRKRRWDLWLCVQNVNVIDKQIRESIAEHVIYINRLDRIKLPFPVGPLL
                     RVLSLGLFKGRLPKMHQAIVKYGAKFNSPKVDDWFYRGHDFYSFYDTTQEYDKAYDKG
                     SYSMLPPGYWRRPIPTAQRNAGFYMRTTKIFFKRFGVINGFAAGALVALVISLPVFGG
                     IAASRHYAQAPTPHIASVTSQPSGQTLMTEFEGYRIATYGELAGQTFYIFVDPDGRRI
                     KSEDLFNRDVLVKDRGPREALLVRGADFLSIYR"
     gene            11077..12486
                     /locus_tag="VFF22_00900"
     CDS             11077..12486
                     /locus_tag="VFF22_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HZX15414.1"
                     /translation="MKPCLFGALTFSQSTGEPMTSRVHSCFCSFLTIVINLFLLLSLH
                     AQSAERVELYDATLQDFVEWSAVQLNKSVVVGSDIRSAPVSIFATYSSDKELEQLLEN
                     AVQSSGFHFSSTPTTIRISSQQIPQSPELVTEVMQLQHLQADFAEQSVRDVLTSRTDN
                     SEPAATQLMVTPSPTTNAIIVTATAQQLDAIRQVITEIDQPRRQVEITAVIAELSDDD
                     FTALGLNVSGQTDRLDLAGASLRASDRSDLGFSLTFSGPTISAFLQAIKTSGSSRLLS
                     TPQLLTLNREAASIVVGQNVPFITGQTTSGSTPASDPFQTIVRQDVGVSLEVTPFITP
                     ADAIELQVNQSASSVSDDKTAADIITNTRRVLTRVQLKDGQGVLLGGLRSQQNDKSVA
                     AVPILGDIPYLGRIFRSESERVRTTNLVVLLTARIHRLGDSVVVPDTTKPWVDLSLWR
                     PGEGLRAAGDLAGRAESAR"
     gene            12553..13836
                     /locus_tag="VFF22_00905"
     CDS             12553..13836
                     /locus_tag="VFF22_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15415.1"
                     /translation="MAKAKDFARLDIETGLENPASRLFVDPGKMGFIDLSGVRLLRCG
                     VDTVRQLYRGMIRPEIMCLFDKPGTLVDFAGQRWHSGRVGKDSGYQYKLQNADLGIIL
                     LVKNFNAKLENIGPHLKIEVSPHAIDRFSPERLQQRLDYYAEHVLTHVERNQCAVHLA
                     LDLQGWTPSADLVARMHCRSRAVRDISGIKEIQWTLESATYGKGQSFMFGSAGSVQIG
                     IYNKTLQARSGDKLDYWEGVWRARDSFDENDPENYDPAQDVWRVELRYHHSVIQQFAS
                     GSVDLHTGKAIETDSFVSFAPHLDGLWRYGLRQFKLLARPGYFEPIWTLFREDVRVDL
                     PVDSLVDKTEYRRSYKTSRGFSGKNVELFLGNFVSLLARERVGARRAFHRLKDWECWP
                     VIRDHYAAKGMDEDGLYKHIKGILEERHVRWGRAV"
     gene            13833..14840
                     /locus_tag="VFF22_00910"
     CDS             13833..14840
                     /locus_tag="VFF22_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003162405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZX15416.1"
                     /translation="MTARKEGKTWTADFYENGRSGRRIRKYGFLTKSAAQRYESDYFS
                     TFNQTGRPLDDRLSDLVDVWYELHGCSLKDEKARLSRTKAIVERLGNPQASQFESLAW
                     ARYRQERLKEASPHTVNHEQRYLSAVFSELIRLGAWVGKNPLANIRQIKTDQTELSFL
                     TLEQINQLLEECRRSSNNHTYPVALICLATGARWDEAESLGRAAIFGGKAHFHRTKNR
                     QSRSVPIPKEVEEAALKVAMPGAGRLFMSCRAAFRGAYERCGFDTPGQLTHILRHTFA
                     SHYMMAGGDILSLQRILGHSSITMTMRYAHLSPDHLESALRLSPLAQSGHVPHGYSVA
                     K"
     gene            14884..15330
                     /locus_tag="VFF22_00915"
     CDS             14884..15330
                     /locus_tag="VFF22_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15417.1"
                     /translation="MESKIPLPTDNIYKFAALFSLVIVVVSFCSIIYSNSAANAIVFE
                     HWVEVETLQAQENLTVEQSSRLKALERKIEVAVSDKQLFIRLAAVFLGLGICGVFFGF
                     GYWHKRIQPLADQMALTQLEMARLQLLLLSAEVKAKGLEAESPKAS"
     gene            15424..15702
                     /locus_tag="VFF22_00920"
     CDS             15424..15702
                     /locus_tag="VFF22_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZX15418.1"
                     /translation="MILNFRCAETQALFEAGDSRRWKNILSVATRKLTMLHAATELRD
                     LRSPPGNRLEALQGNRVGQYSIRINDQFRICFEWSDAGPTNVEIVDYH"
     gene            15715..16020
                     /locus_tag="VFF22_00925"
     CDS             15715..16020
                     /locus_tag="VFF22_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HZX15419.1"
                     /translation="MAVNGMRPIHPGEILREEFLEPLDISPAALARALHVSAPTVNEI
                     VRERRGVTADMAIRLGRYFDTSAQFWMNLQTDYSLATTFAANGEEIEQEIEPLLAHG"
     gene            complement(16102..16178)
                     /locus_tag="VFF22_00930"
     tRNA            complement(16102..16178)
                     /locus_tag="VFF22_00930"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16142..16144),aa:Met,seq:cat)
     gene            complement(16256..17356)
                     /gene="ychF"
                     /locus_tag="VFF22_00935"
     CDS             complement(16256..17356)
                     /gene="ychF"
                     /locus_tag="VFF22_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HZX15420.1"
                     /translation="MGFNCGIVGLPNVGKSTLFNALTKSGIAAENFPFCTIEPNSGIV
                     PMPDPRLDALAEIVKPERVLPTTMEFVDIAGLVEGASKGEGLGNKFLANIRETDAIAH
                     VVRCFQDDNVIHVANSVDPKRDIEIIDLELIFADLDSCEKQLQKTTRNAKGGDKEAVA
                     QKALLEKLIPHFSEGKPARTLMKTLGDDERALVRGFHLLTSKPVMYIANVAEDGFENN
                     PLLDVVQAIADAEGAPVVPVCNKIEAEIAELDDGEEKDMFLEALGLEEPGLNRVIRAG
                     YELLNLQTYFTAGVKEVRAWTVKIGATAPQAAAVIHTDFEKGFIRAEVIAYNDFIQYK
                     GEAGTKEAGKWRLEGKEYIVKDGDVMHFRFNV"
     gene            complement(17390..17980)
                     /gene="pth"
                     /locus_tag="VFF22_00940"
     CDS             complement(17390..17980)
                     /gene="pth"
                     /locus_tag="VFF22_00940"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HZX15421.1"
                     /translation="MTAVQLIVGLGNPGPEYDQTRHNAGALFVERLADSQRINLSLDK
                     KYFGLTGKFSHQGRDVRLLIPTTFMNRSGQAVAALANFFRIPPEAILVAHDELDMPPG
                     VAKLKLGGGHGGHNGLRDIIAQLGNQNNFYRLRLGIGHPGHSSMVSNFVLGRAPRSEQ
                     ELLDTSIDFALGALPEILAGDWSKAMHKLHSQKATS"
     gene            complement(18050..18661)
                     /locus_tag="VFF22_00945"
     CDS             complement(18050..18661)
                     /locus_tag="VFF22_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HZX15422.1"
                     /translation="MTVEFALNAEVRSDLGKGASRRLRRNVAMVPAVIYGGEKAPQSI
                     SLLAKELAKLLENEAAYSHVLSLNVAGSNESVIIKALQRHPAKGYVLHADFVRVVAGQ
                     KLTAIVPLHFINQETSVGVKQQGGEVSHVLAEVEVSCLPKDLPEFIEVDMAAVEIGQI
                     VHLADLKLPKGVELVALAHGNDLAVANIHASRVVKDAEEGAAE"
BASE COUNT         3875 a         5161 c         5057 g         4702 t
ORIGIN      
        1 acgcgcatcg atgcaggaca cccagttcgg cctgatcgac ggttggctgc gcagcattcg
       61 cgacctctac tacgagcacc gcgaacacct ggcgcagttg cccagcgaag aagagcaggt
      121 cgatcgcctc tgtgagctca atgtgatcca gcaagtggcc aacgtcagcc acaccaccat
      181 cgtgcagaac gcctggcatc gtgggcagcc gctggcggtg catggctgca tctacggcat
      241 caaggacggc cgctggaaga acctcaacgt caccgtcagc ggcatggaac agctgccgcc
      301 gcaataccgc ctgcgcccgc tcggtcagcg ctgagtatgt tgtcttccgt gcgtatcgcg
      361 ctggccggcc tgctgctggc gacgctgtgt tggtccggca atgctttagt agcgcgagcg
      421 tttgccggtg agattccacc gtttgccttg tcattctggc gctggagcct ggcgcttgtg
      481 ctgctgttgc ccttcgtggc caagccgctg tggcaacacc gcgctgcact gcgtgcggcc
      541 ggctggcgtt tgctggtgct tgctgccgtc ggtattgcgg gctacaacag cctgctctac
      601 gccgccgcgc aaaccactgc tgcgattaat atcaccctgg tcaacacctg cctgccactg
      661 atgaccttta tcggtgccgg tctgctgctc ggcgagtggc cgcatcgacg cgcctggtgg
      721 ggcatggcag tggccgcagg cggactgctg gtgctgatca gcaaaggcag ctgggccagc
      781 ctcagtggcc tggccttcaa caagggcgat ctgatcatgc tgctggcggt ggccgactgg
      841 gcgctctact cattgctgct acggcgctgg tctgcccacc tgctgccgat tccgccactg
      901 gccctgctcg gcgtgctgat gctgctgggc gtgccgctaa ttttgccgtt ctatctctac
      961 gagctggctc agggcgcaca cttcaagctg acggcagaga acttggccgc catcagctac
     1021 accgcgatct tcgcctcgct gctcgcctac ctggcctgga accacgccat ccgcgtactc
     1081 ggcgccgcca aagcagcact ctccaactac ctgatgccgg tgtttaccgc cgtactgggt
     1141 tggttgctgt tgggcgaggg cttgcagagc tatcactggc ttggtgctgc gctgatcttt
     1201 gtggggctgc tgctgggtac gcaggggcgt cgttaagcgg agaagtcaga cgtgatttac
     1261 acgtttgtcg ccatcgctgg ggggcaacta gttgattcat tcccgccagc aaagctgggt
     1321 gatcacttcg ccacggcgct gaccttctcg ccgtcggctt tgctggagac ctcctaaggc
     1381 tggtgagcct gttggtaacg accttctctg ctctttctat ggtctgaacc ttcttgctgt
     1441 tagtcagaaa gcacgacaaa gttaagtctt ctttgagctg cattttggtt gcctcatgac
     1501 tgaacgttcc accagatcgt ccgggaagcc ctctctcaat gagcaatcgc aggagaattt
     1561 tgccgataac gaaggctgga tcgcatggcg aaacttgacc actgaagcca tgaggcgttt
     1621 tcacgagcag cttcttgcga acgtttccag ggccccgggc ttggtgggtt acgaggcagc
     1681 ggaacgcgca cgggcagcta atgggagcgt tgcctggtct tggggggcag ctgctgaaat
     1741 tcaggaaact atcaatacga taaattcgtg ggggacgcac cttcatgatt ggtgcgcttg
     1801 gaatgccgtt gttgagtcat ttgagacgga tgaagataaa gggcagctgc tgcatcactt
     1861 cgttaacccc ttagctttct tctgcatgca tcaacctagc gcagtcagtg atcgcctgat
     1921 gcttcttgcc gagacacttt tacatcaggc gaactgtctt attgaaccag gctacgttga
     1981 tcgacttgat caggatggtt tgagacatgg acaagggctt cgacgctctg atcgcagaaa
     2041 gcaggtcatt cgcctcggtc accgctggac acgatttaat gggtttctgg ctgcgttgga
     2101 tgcaatcaat gactctgcct atcgaaagct tagtcgcaat ttccgcgatc tatctgttca
     2161 ctcatttgca cctaggttca tggttgggca aatcatgcgc gcatttcgtt cgattgagcc
     2221 ttggcaagaa ctcgtggagc aaccgggcgg tggctcctta cttgtagatc atccaactaa
     2281 gaaatgtgtc agctacacga tgtgtgtgct ggaacctttt cctctccgcg atgcatgctc
     2341 agctagtctt tctgagtacc aaaaagtagt ggctgctatg agcgcatttt ctcagctgct
     2401 tgaggaggta tgcgacagca tggatgcgat accagatcgc ttatctggac agtcgtaggc
     2461 tgggacaaga ttgagacact tgtgggacac ccagaaaaca aaaaagggct tagctttcgc
     2521 taaacccttg ttttgtttgg tggctacgca gggacttgaa ccccggaccc cagcattatg
     2581 aatcgcttga gggtgaccat agtgaacctt agcagcccat aaaacggggc tctcagcagg
     2641 ccttcgacct caccagaaca gagctttcca gaaaaaagtg ctgatgtagt catcagcttt
     2701 taagtcaaaa agcctttagc tgtcagttca cttgcaaggc atcttgctag tcctgaaaat
     2761 tctttttcat cagttaactc tgacttgaaa tcgcttttaa gtgtttcaac taattgtgga
     2821 tggttagttt tggctatgac atctagcatc agcattaagt actcaatttt ttcttgtctt
     2881 ttttcagtta gagcttctct gtttagcgct agttcttcaa ttgtatattt tgaaatttca
     2941 tcgccaggtg caggccaaat agttgggcct ataaatagca gtctatctgc agggtcattt
     3001 tcatagggat ctaggatgtg ctctttgctt gatttttttg tattgcaaat atcacaagct
     3061 agagtcaagt tgtcccagtc aaaccaaagc tctggtctga ttttttttgg tgtaatatgc
     3121 tcaatgtctc cataggttat atgaagtatt ttactttcgc agtaagcgca ctttccgtta
     3181 gtttctttaa ccagagcttc ttttatactt ggatggttgt acttattttt ttctgatttt
     3241 gctagttcaa tagattgttt taatttttca agtacagcgg ttgtccattt ggctgcattg
     3301 tcttttaaaa catctggtat ggcgcttttt tttagtttaa tcattttcct ttcataagct
     3361 ccgcgagagc cagcgggtaa tgatcactaa agcctaagtt ctttagctct gctcttatgt
     3421 tttctagtgt ttctttcgtt atttctaagt ttttgtattt ttttgttatt atgcttagtt
     3481 cttcagatac ccactctggt gtcgttgctt ccacccctaa aacttctcta agtatctcgt
     3541 ttgcactaga tgccttgttt acggtgtcta gcttttcact tataactctg cttttggttg
     3601 tttcaggggc gaacccatca actactctgt cttcagtgtt gtatctaagc acatagacat
     3661 aggagtcttt aactgatgaa attataaatg gactatgtgt tgcaactata aactgcacct
     3721 tagggaaggc gcttagtaaa cgttgcatga ggcttctttg cattgatgga tgtaaatggt
     3781 tttcaggttc atccatggtg acaacaaaac tctcattttt taacgagaac atatgcaatc
     3841 gccaagctag gtctattagt gtcgaaacac caccagatgc agcgtctagt aggaattcac
     3901 ctgtctttgt ttgtagtact acctctggcg ttcttacaga aagatcaata aagcctaatg
     3961 atgcaggtaa tactttatgc agcacgttta taaatccgct taacgccaac tgcagctctg
     4021 ggttcccctg gtttctagag ttaccttctc caaaaaccgc cattgatatt atggcttctt
     4081 tcattcggta tagcggcgaa tacccggtat gcccaccatg gtatcggtta tttatttcgt
     4141 tatggtacag gtcatatgct gcactagggc tcgtcatact tgacgggatg ttagctactt
     4201 gtcggtagtt tgatataatt tgatgtgagt ctatgtggat tccgctaatt ccctgcatgc
     4261 cgtttatttg taatagatat tgcgcgctgt cgctttcttg tactgttaga gatgactcgg
     4321 ttttgttggt gtagcttatt ttccctaccg ttgagtggtt ttctttttta aaaaaactgc
     4381 ttattttttt tgttaaggat ataaatagtc cggttttgta aacaaatctc ccttccacta
     4441 gaactggtgt tgacaagaat ggtagctcta ggccgaagtg ttttgagaat gcttttagta
     4501 tcgagctttt acctgctcca tttgctcctg tgactattgt aagtctctcg tgcagatcta
     4561 tttcgatatt gtcgaattgc ctccatccct gcagtgcgat tctcttgaat tttatccctt
     4621 ccattttagg ctcttttaat ttgatttaaa ttttcagtat gttgtgaata tagctaccac
     4681 tctttcactc tcatcccatc aatgcactcc ttcacgtcat catcaatgac gttgccaggc
     4741 ttgatgcatt ccttcatggt gcggcgaact ccgcgattgg cttcgagttc tcgttgattt
     4801 tgggatttga ggttggagcg cgcggcctcg cctaacgggc tttttgaccc ggcgaagaac
     4861 tcattcacca gcgtattaac tgttttgccg taggcttcgg cggcggggcg tagtgcttcg
     4921 cctacttggg tggcgacggg ttttgactct tcggctaggg cagggcttga ggctagggcc
     4981 agtgccagca aaatccttct catcattctt ctccattcct tcgggcatac cagcgcctag
     5041 ctactaattg ggtaatcgct atcccgcgtt tggactggtc aagtttcggt cagcctcttc
     5101 atagtccggg ctgatctcgc ctttagctgg atcaattcgt cctgttctca accacagaga
     5161 gtattgggga tagcgatctg ccatcaagtc catttcgctt gtcctcaggt tcgctctctt
     5221 gtcgtacctg atggttttgc agcgttcttc ctttatcccg gtggcttcgc tgacctcgcg
     5281 atgaccaatc ttagataaaa aaagcctaac tctatcgtgg gttgattcca taaagatctt
     5341 aaaaatctct ggttgaaata ttccactcgc cttaagcgat ggtattattc cactcaattg
     5401 gtggaaatat tccacttgtt gttctgagtg cttctgggat tatagggatt tttatatgga
     5461 acagtctggt gtagtagggc tcaacatcga agggaacccg gagcgcgtta ccgattttcg
     5521 tgacgcgccg ttttgctcca tgcaggttct ggtcgacatg ctcggcacag agctcattag
     5581 ccctgacgtt gtgcgcggct gggttgagac ttccactctt ccaaccatca agatcggccg
     5641 ccgccgtgta atcaacctcc accgcatccg ccgcgacctc gaccggggca aaaccgtgtt
     5701 ctgcgccggg gattacgccg atgagtaaga ctctctatat cgtcgaaacc tgcaccctcc
     5761 acggcgctac caaacagcgt cgctggcatc gcgtgcatac cgggccgaac aaagccgaat
     5821 gcagctccta tatagagcgt gtcattgctg acctaccgtc aggccctggt cgtcactttg
     5881 gactcacaca agagcgtgcc cgcgacttct atcgtgtgcg tggtgtgagg gctgcagcat
     5941 gactattcgc actgtttcag gctttccggg cgagggcatg agctatgacg ccatccagct
     6001 acagcaacca accgcacgca ttgaactgcg actgctctgt ctgctggtca aaggccgaga
     6061 tggccaagcc agatccttgc ccgtccacac cctgcgacca atgtcgcccc gcatctgtcc
     6121 aacgcggccg catcctaagc atgggcctcg tcgctggtgt ttggatgcct ttgaagtggg
     6181 aaccgctaat aaccccggcc ttgtattgcg cgaaacacaa gccaagcgac cgaccgccga
     6241 agtattggag cgttgtggaa aacatcggca agccggtgcc cttcgtgccg ctgcacgaac
     6301 ctttcgagct gacggggtga gccatgcaat gggttgaagt gatcaccctg tctttgtcct
     6361 ttatgtgcgt gggcatcctg attggctatt ccttcggtcg tgtcgctcag gcacgtgctg
     6421 ttaaggctga tccatttctt gcctataccc gcgctgaact caagggtttc acctgcggca
     6481 tgctcgctgc gctagtcgcc attcacagca agaccgaccc acccagtaga tcgccgcctg
     6541 tgtccgtgac tggccaacgc ctcaactccg gtcagcctac ggagtcaccg ccagtcgcgg
     6601 gcgcaggtgg cgaaacggga tgacaagggc gaggcccttg gtgttatcgg tttgtattaa
     6661 tgaagttaat attcggaagt taagaaacct cactaataca gtctgttcca gagtaattcg
     6721 cgcaacatga taaatattga atagtgaaaa taacgcttgt ttctgtgtat taataagcac
     6781 gcataaccca tcgcaagccg caccacacct agcagataaa gaactttagc agttcttctg
     6841 cttgggatta ctcggcctgc agaaaggcaa aaccgcgcac taacgcgcaa ctaagcgagg
     6901 aaacaaaaga tggcacgttc aactatggaa gttgcatttc ttggcactca acgcttcgac
     6961 ggcgaaaacg gccagaagta catcaaggtg ttttatggcg atgaaccgga cggcaaaacc
     7021 gagcacggcc tgtccatcat cggcatggct gcagaggagg atgctgcaga agaaatcttc
     7081 gccgctggca ctcgcttcga acccctgcaa ctggtgcgca tcaccttcga cgtggcccgg
     7141 ggtggccaga acaagggcaa gaacctagct ctgcacatcg aagcggtaga taccaaagcc
     7201 actcgcgccg cctccccaca agccaacgcc cagcagcagg ccaaacccgc tgacccggcc
     7261 aaggcctgag gctgaccgcc gtgctgatcc gtgatcgtgt gctgtgcgat gagtgcatgg
     7321 ggcctatggg ccagctgcat cacatgcctg tcgatcacaa cgaccacttc acggacaaca
     7381 gcacggcccc cgattactgc gtgtgccctg actgccagga cgccttagcc gctcaagcca
     7441 gccagcaatc aacggctggc gactaagcac cttgcagcag gccacgcaca accccggagg
     7501 catcaggtat ggacagctat ttagccagca tcaccttggg cgacctctgg gcgctccagt
     7561 tcattcaagg cgtcgtgacc atcatggccc ttggtttgat tcacgggcat cagaggtaac
     7621 gggctatggc tttcacctgg gggcaatacc tcgttatcgc tgggcttctc gtgggttccc
     7681 tgggtatcgg cgtcgcctgg ggtgctttcc ggctcggctg gaaagaactg atagacgcat
     7741 caacaagctg aggatgtacc tatgtaccaa agcaaaaaag aagtcgccat tcgtggcggc
     7801 cagctcgtac agcaagaagg caccagtgtc ggtaaagcac tgggcggcaa agccgtggct
     7861 ctcttcctga ccggttccgc tgccctgacg gcgggttctg caaacgcggc catcaccgtt
     7921 ccacctgagt tgctggaagt cttcaccgat ctggccaccg ccttcggcac cctggtcgca
     7981 gccggtgcca tcctgttcgg tgtgattcgc ggcaccttgg ccctgttcaa actggctcgc
     8041 agcgtcttcg gcgcggctgg ggcttaacag gccaggaggc taatagatgc gcgcggctat
     8101 tggccttcgt ttgctgcttg ctctcactct tatggggtgg gggcaattgg cgttatttaa
     8161 atttattagc ttttcccgct atttcttcgt tttttccctg gttgcattgc ctatttattc
     8221 ttatgcctca tctcctgtcg ctgtttatgt taggcctggg cattcaaccc ctaattggcc
     8281 tactgcagaa gcggcgtgta aggcgcttta tggttctact gcatatgttg cctttcaggt
     8341 tggctgttat gctccgccta gtggaaacta tctcggcagc gtgcaaaaga ttcatatttg
     8401 cacaacaggt actctcgacc ctgctactaa tcaatgcgta gttcctgaac cacccaagga
     8461 ctgctcatcc cataacggca aattcattat tgccgctgtt tctggctctc aggccggaag
     8521 tgcttattca acgcctggca ctgtttcaat cgatggtgct aggtatgttt cagcgcctga
     8581 cggcaacacg cgctgcaagc caaattttga cggttcgggc acggccattt gcgccgctcg
     8641 atatggcggt gatggctcgt gtatgccctc tggccattcc gaatctgatg acttaactga
     8701 aggcaccttt gtcgatgtca ccgacacgtc aaccggtgat gaaaagtgcc tgacgttcgg
     8761 cggcaaagag ctgtgcttgt cgcctgacaa cgccggctgt gatgtctata acggcaaccc
     8821 ctggtgctat cagaccggcg ataactgcgg cgaggtaact gagcctggca caactcagag
     8881 caaatttgtc tgcttcccca aaacagcccg tcaatgcacc tacgccaacg gcaagatgga
     8941 atgtatcgaa accggcaacc ctccaggctc taagcctcct gcctacatta ccccgacgtc
     9001 ctccgatcac ccctacaacg gcggcaacgc tgatgggaat gacaagaacg atccgaaagc
     9061 ccctggcgcg ggtgacgaca cccagtatgt cggcggcggt ggcccgccca ctgatttaag
     9121 ccagaccaat gatgaacttc gcggcctggg tgaaaagctc gacgaatcca ccggcctgct
     9181 tgaagacatc aaagatttat tgaccggcga cggtgatgac tcgggcaatt ccgatggttc
     9241 tgcagccgat gccgaagccg aaggtgctgc agttggtaat caaattggcg atgccatcac
     9301 cacccagatg gaagccgtcg acgaagagcg caatgaagag gttgaaggcg ttcttgatga
     9361 actgcccaac caagtcagcc agtggtttgg cgcggatggc tcagccgttg gcctcgactt
     9421 tatcgacagc attcttccta cgcctgttgc ctgtgctgat tacgtcgtgc cgctctccct
     9481 caatggctac accgccaatt tgcgtatgcc ggtctgtgcc ctcatgccgt acaagccgat
     9541 tttggaatgg gtcatctggt gcctgacagc tattggcgcg tggcgcattg gttattccgc
     9601 tctgcgccag gaagacgcta aagccgtcaa aggaggtttc tgatgggcta tttcctcaac
     9661 ttcttcaaga agctatttcc caccgcattt tcctggctgg ccaagtttct gctgggcctc
     9721 atcatgccca tcctggcgcc ggtcattcag gccgtggccg cactgctgcg caagatcggg
     9781 atcttcctgc tgatcatcgc cgccattggt gcggccattg ccgtgctggc tgcaggtatc
     9841 gactacgtgg tgtcggggat tggctcttta gtcgcccctg atctggtgat cgtcggccgc
     9901 atgtttctcc ccagcaacct gtcgttctgt atctccttgc tggtgtttct aaaactcaag
     9961 tccctggttt tctactgggt gaccaagctt tccgaaaaac tcattcacac ctgagcactg
    10021 ccatggccgt ctatatcgtg accggcaagc taggggccgg gaaaaccctg ctgtgtgtga
    10081 tgcgcatcct cgattacctc aagcaaggtc gccgagtggc ggtgaacgtt gatgtgttca
    10141 tggacaagct gtgtaagcgc gacaacaaac attcacggct tgtgcgcttg ccggatctgc
    10201 ccaccgctga tgacctaacc ggcctgggtg ttggctgtga tgtgtacgac gaagaaaaat
    10261 tcggcgggat cttcctcgat gaagccggcg tttggctcaa ctcccgcgac tggaacagcg
    10321 gtggccgtac cgacctgctc aagttctttc tgtttctacg caaacgccgg tgggacctgt
    10381 ggttgtgcgt gcaaaacgtc aacgtcatag acaaacagat ccgcgaatcg attgctgagc
    10441 atgtcatcta catcaaccgc ctcgaccgca taaagctgcc gtttccggtc ggcccactgc
    10501 tgcgcgtgct gtccctcggc ctcttcaagg ggcggttgcc gaagatgcat caagccatcg
    10561 tcaagtacgg cgccaaattc aactcgccca aggtcgatga ctggttctac cgggggcacg
    10621 acttctacag cttctacgac accacccagg aatatgacaa agcctatgac aaaggctcct
    10681 actcgatgct gccgcctggg tattggcgtc gccctatacc cactgcccag cgcaacgcgg
    10741 ggttttatat gcgcactaca aaaatcttct tcaaacgctt cggcgtcatc aacggcttcg
    10801 ccgccggtgc actggtcgcc ctggtcatca gcctgccggt tttcggtggc atcgctgcat
    10861 cacgccacta cgcccaggca ccgactccac atatagccag cgtcaccagt cagcccagcg
    10921 ggcaaaccct gatgactgag tttgagggct accgcattgc tacctatggc gagctggccg
    10981 gccaaacctt ctacatcttt gttgacccag atggccggcg catcaagtcc gaggatctgt
    11041 ttaaccgtga tgtgctggtc aaggatcgcg gcccccgtga agccctgctt gttcggggcg
    11101 ctgactttct ctcaatctac aggtgaaccc atgacctctc gtgtgcacag ctgtttttgc
    11161 agcttcctca ctatcgttat caacctgttc ttgctcttgt cactacatgc acagtccgcc
    11221 gagcgcgtcg aactgtatga cgccaccctg caggatttcg ttgagtggtc agctgttcag
    11281 ctcaacaagt cggtggtggt cggctcggat atccgctctg caccggtctc catcttcgcc
    11341 acctacagca gcgacaaaga gctagagcag ctgcttgaaa acgccgtgca atcttccggt
    11401 tttcacttct cctcaacgcc gaccaccatc cgcatcagct ctcagcagat cccgcaatcc
    11461 cccgaactgg ttactgaagt gatgcaactg cagcacctgc aggcagactt tgccgaacag
    11521 tcggtgcgcg atgtactcac ctccagaacc gacaacagcg aacctgcagc cacccaactg
    11581 atggtcacgc cgtcacccac cacaaacgcc atcattgtca ccgccaccgc ccagcagttg
    11641 gacgcgatac ggcaagtcat caccgagatc gaccaaccgc ggcgacaggt ggaaattact
    11701 gcagtaattg ccgagctgtc cgatgatgac ttcaccgcac tcggcctcaa cgtcagcggc
    11761 cagaccgacc gcctcgacct ggctggggct tcattgcgtg caagtgatcg ctccgacctc
    11821 gggtttagcc tgaccttttc agggccgacc atttcggcct tcctgcaggc catcaaaacc
    11881 agcggtagca gtcggctact gtccacgcct cagctgctca ccctcaaccg cgaagccgct
    11941 tccatcgtcg ttggtcagaa cgtcccgttt atcaccggcc agaccaccag cggctctacg
    12001 ccggcctctg atccctttca aaccatcgtg cgccaggatg taggtgtctc cctcgaggtc
    12061 acgcccttta ttaccccggc cgatgccata gagctgcagg ttaaccagtc cgcctccagt
    12121 gtttcggacg acaagaccgc cgccgacatc atcaccaata ccagacgggt gttaacgcgg
    12181 gtgcagctca aggatgggca aggcgtgttg ttgggtgggc ttagatccca gcagaacgac
    12241 aagtctgtgg cggctgtgcc gatcctcggt gacatccctt atctcgggcg gatcttccgt
    12301 tctgagtctg agcgggtgcg cacaaccaac ctcgttgtct tgctgaccgc ccgtatccat
    12361 cgcctggggg attcggtggt ggtgcctgat acgacgaagc cttgggtaga tctttcgctc
    12421 tggcggcccg gcgagggttt acgcgcagcg ggagacctcg ccgggcgcgc cgagagcgcc
    12481 cggtgacgtc cctgtagcac gtcagataaa caaaacagta gaaccagact aaaccagagt
    12541 attgggtgaa aaatggctaa ggcaaaagac ttcgcaaggc tcgacataga gaccggattg
    12601 gaaaacccgg caagccggtt gtttgttgat ccgggcaaga tgggtttcat cgacctgtca
    12661 ggcgttcgcc tgcttcgctg tggcgtcgat accgtccgcc agttgtaccg gggcatgatc
    12721 cgcccggaaa tcatgtgtct tttcgacaag cctggcacct tggttgattt cgcgggtcag
    12781 cgttggcatt cgggccgtgt gggtaaagac tccggttatc agtacaagct gcaaaacgct
    12841 gacttgggca tcatcctgct cgtgaagaac ttcaacgcca agcttgagaa catcggccct
    12901 catctgaaaa tcgaagtgtc gccccatgca atcgaccgct tttcacccga gcgcctgcaa
    12961 cagcgtctcg attattacgc cgagcatgtg ctgacccatg ttgagcgcaa ccagtgtgcg
    13021 gttcacctag cgctagacct gcaaggctgg acgccatctg ctgacctcgt cgcacgcatg
    13081 cactgccgtt cacgcgctgt gcgtgatatt tcgggtatca aagagatcca gtggacgttg
    13141 gagtctgcca cctacggtaa ggggcagtcc ttcatgttcg gctctgccgg tagcgtccaa
    13201 atcggcatct acaacaagac cctgcaggcc cggtcgggcg acaaactcga ctattgggaa
    13261 ggggtttggc gtgcccgcga tagctttgat gaaaacgacc ctgagaacta cgacccggcc
    13321 caggatgttt ggcgtgtaga actgcgctac caccactcgg ttatccagca atttgccagc
    13381 ggctcggttg atctgcacac cggcaaggcg attgaaacgg acagctttgt ctcttttgca
    13441 ccgcacctcg acggcctttg gcgctatggc ctgcgccaat tcaagttatt ggctcgccct
    13501 ggctactttg agcccatctg gacgctcttt cgtgaagacg tgcgcgtaga ccttccggtc
    13561 gattccttgg tggataaaac cgagtaccga cgaagctaca agacctcgcg gggcttctct
    13621 ggcaagaacg tggagctgtt cctgggaaac ttcgtaagcc tgctggcacg ggagcgagtg
    13681 ggcgctaggc gagcatttca ccggctcaag gattgggagt gttggccagt gatccgcgac
    13741 cactacgcgg caaaaggtat ggatgaggac gggctgtata agcacataaa aggcatcctt
    13801 gaagaacgtc atgtccggtg gggtagggcg gtatgactgc ccgcaaggaa ggcaaaacgt
    13861 ggacggctga tttctatgag aatggccgct ctggccgtcg catcaggaag tatgggtttc
    13921 taaccaagtc tgcagcgcag cgctatgagt cggactactt ctcaaccttc aaccagaccg
    13981 gccggccttt ggatgatcgt ctgtctgatc tggttgacgt ctggtacgag ctgcacggct
    14041 gttcgctcaa ggatgagaaa gcccggcttt ccaggactaa agccatcgtg gagcgcctgg
    14101 gcaaccctca ggcctcacag ttcgaatcct tggcctgggc tcgctatcgt caagaacgcc
    14161 tgaaagaagc atcaccgcac acggtcaatc atgagcaacg ttacctatct gcagtgttct
    14221 ccgaactgat ccgcctaggt gcttgggtag gaaagaaccc gttagccaat attcgccaga
    14281 tcaagaccga ccagaccgag ctgtcatttc tcaccttgga gcagatcaat cagctgctcg
    14341 aagagtgtcg ccgatccagc aacaaccaca cttatccggt tgccctgatt tgcttggcca
    14401 ctggtgcccg ctgggatgag gccgaaagcc taggccgcgc tgccatcttc ggtggtaaag
    14461 cgcatttcca ccgtaccaag aaccgccaat ctcgttcagt gccgataccc aaggaagtgg
    14521 aagaggccgc gcttaaggta gccatgccgg gagcagggcg cttgtttatg tcctgcaggg
    14581 cggcttttcg tggtgcctat gagcgttgcg gcttcgacac gccgggccag ctaacacaca
    14641 tcctgcgcca taccttcgcc agccattaca tgatggccgg tggcgacatc ctcagcctgc
    14701 agcgaatcct tggccattcc tcgatcacca tgaccatgcg ctatgcacat ttgtcgcctg
    14761 atcatctgga gtctgctttg aggctttccc ctttggctca atcaggacac gtaccccatg
    14821 gctattcagt tgctaagtaa aatccgggca tacttcgctg gcttaattca gtcgaggtgt
    14881 tacatggaaa gtaaaatccc gttgccaacg gacaacatct acaagttcgc agctctgttc
    14941 tcactagtga tcgtggttgt ctctttctgt tcaatcatct attcgaatag cgccgccaat
    15001 gcgattgtgt ttgagcattg ggttgaggtg gagaccttgc aagctcagga aaatctcact
    15061 gttgagcagt cttcaaggct taaagcattg gagagaaaga ttgaggttgc ggtgtccgac
    15121 aagcagctct ttatcaggct ggcagccgtc tttcttgggc tgggtatttg tggtgttttc
    15181 tttggtttcg gttactggca caagcggatt cagcccttag cagatcagat ggctttgact
    15241 cagcttgaga tggcgaggct gcaactccta ttgcttagtg ccgaggtaaa ggcgaagggc
    15301 ttggaagcag aaagccccaa agccagttag tcccctcatt tccttctcta cgccatgcga
    15361 ccgcttgaaa accgtctgta atggcgtagt atgtctataa cgcttaacgt taagggttaa
    15421 agcgtgatcc tgaatttcag atgtgcagaa acccaagcgt tatttgaagc gggcgattca
    15481 cggcgctgga aaaatattct gagcgttgcc acccgtaagc tgaccatgct ccatgccgcc
    15541 actgagcttc gagacttacg ctctccaccg ggtaaccgtc tagaagcgct gcaaggcaac
    15601 agagttggtc agtacagcat cagaattaat gatcagttcc gcatctgctt tgaatggtca
    15661 gacgcggggc caacgaacgt tgaaatagtt gattaccact gaggaggtcg caccatggcc
    15721 gttaacggaa tgcgccccat ccatccggga gagatcctgc gcgaagagtt cctagagccg
    15781 ttggacattt cgccggctgc gcttgctcgt gcactgcatg tatctgcgcc aacggtaaat
    15841 gaaattgtgc gtgagcggcg gggcgtgact gcagatatgg ctattcgcct ggggcgttac
    15901 ttcgacacgt ctgcacagtt ctggatgaac ctgcagactg actattcgtt ggctacaacc
    15961 ttcgcggcta atggcgaaga gattgagcaa gagattgaac cgctactcgc tcacggatga
    16021 gcagtagtcg ggatgtagtc tgtttgtagt ctgcccgtaa aacaaaaaag ggcttacctt
    16081 tcggtaaacc cttgttttgt ttggtggcta cgcagggact tgaaccccgg accccagcat
    16141 tatgaatgct atgctctaac cagctgagct acgtagccga ttggcgcgca ttattcgcgt
    16201 cttggggttg ggtgtcaacc ctgttgagtg aaaatttctg cgcgcgatca atcgcttaca
    16261 cgttaaagcg gaaatgcatg acgtcgccgt ctttaacgat gtattccttg ccttccaggc
    16321 gccatttgcc ggcctctttg gtgccggctt cgcccttgta ttggataaag tcgttgtagg
    16381 cgatgacttc ggcgcggata aagccttttt cgaagtcggt gtggatcacg gcagccgctt
    16441 gcggggcggt ggcaccgatc ttgacggtcc aggcgcgtac ttccttgacc ccggcggtga
    16501 agtaggtctg caggttgagc agctcgtagc cggcgcggat tacgcggttg aggccgggtt
    16561 cttccaggcc cagggcttcg aggaacatgt ccttctcttc gccgtcgtcc agctcggcga
    16621 tttccgcttc gatcttgttg cacaccggta ccacgggcgc gccttcggcg tcggcgatgg
    16681 cctgtaccac gtcgagcagc gggttgttct cgaagccgtc ctcggcgacg ttggcgatat
    16741 acatcaccgg cttgctggtg agcaggtgga agccgcggac cagggccctt tcgtcgtcgc
    16801 ccagggtctt catcagggtg cgtgccggct tgccctcgct gaagtgcggg atcagcttct
    16861 ccagcagggc tttctgcgcg acggcttctt tgtcgccacc cttggcgttg cgtgtggtct
    16921 tctgcagttg tttttcgcag ctgtcgaggt cggcgaagat cagctcgagg tcgatgatct
    16981 cgatgtcgcg cttggggtcg acgctgttgg cgacgtggat cacgttgtcg tcctggaagc
    17041 agcgcaccac gtgggcgatg gcgtcggtct cgcggatatt ggcgaggaac ttgttgccca
    17101 ggccttcacc cttggaggca ccttcaacca ggccggcgat atcgacgaac tccatggtgg
    17161 ttggcagcac gcgctccggc ttgacgattt cagccagtgc atccaggcgc gggtcgggca
    17221 ttggcacgat gccgctgttc ggctcgatgg tgcagaacgg gaagttctcc gccgcgatcc
    17281 cggatttggt gagggcgttg aacagggtgg acttgccgac gttgggcagg ccgacgatgc
    17341 cgcagttgaa tcccatggtg ttgtccctcg cgcgaatggc ggtaaagagt taggaggtgg
    17401 ctttctggct gtgcagcttg tgcatggcct tgctccagtc gcctgcgagt atttccggca
    17461 gggcgcctag ggcaaagtcg atgctggtat ccagcagttc ctgttcgctg cgtggggcgc
    17521 ggccgaggac gaagttggag accatgctgc tatgcccggg atggccaatg ccaagccgca
    17581 ggcggtaaaa attattctga ttgccgagct gggcgatgat gtcgcgcagc ccgttatgcc
    17641 ccccgtgccc gccacccagt ttgagtttgg cgacgcctgg gggcatgtcg agttcgtcgt
    17701 gggccaccag aatggcttcc ggcgggatgc ggaagaaatt cgccaatgcc gctacggcct
    17761 ggccgctgcg gttcataaag gtggtgggga tcagcagacg aacatcgcgg ccctgatggc
    17821 tgaatttgcc agtcaggcca aaatactttt tgtcgaggct caggttgatg cgctggctgt
    17881 cggccaggcg ctcaacgaaa agggcccctg cattatgccg ggtctggtcg tattcagggc
    17941 cggggttacc caggccaacg atcagttgta cggcagtcat gtcaggagcc cttttgtgga
    18001 aatagctcgg cagaatcgca taccgagctg gtttccgtca ctgcgaggtt tactcggcag
    18061 cgccttcttc tgcatcttta acaacgcgcg aggcgtggat gttagccact gccaggtcgt
    18121 taccgtgagc cagtgcaacc agctcaacac cttttggcag tttcagatcg gccaagtgaa
    18181 cgatctgacc gatttcaacg gcagccatgt cgacttcgat gaattctggc aggtctttcg
    18241 gcaggcagga cacttctact tcagccagta cgtgggaaac ttcgccacct tgctgcttaa
    18301 cgccaaccga ggtttcctgg ttgatgaagt gcaggggcac gatagcggtc agtttctggc
    18361 cagcgactac gcgaacgaag tcagcatgca gcacatagcc tttggctgga tggcgctgca
    18421 gggctttgat gatcacgctt tcgttgctgc cggctacgtt caggctcagc acgtggctgt
    18481 aggctgcttc gttttccagc agtttggcca gttctttggc cagcaggctg atcgactgcg
    18541 gggctttttc gccaccgtag atgacggcgg gaaccatggc aacgttacga cgcaggcggc
    18601 ggctcgcacc tttccccagg tcggaacgca cttcggcatt cagggcaaat tcaacagtca
    18661 tttcacttct ccaaaataac caaaccgtcc gttacgcttg cgaccagcga cgggacggtt
    18721 cagtaataag cccgtactta aaatgagcag ggcgtttgcg ctcagggcgt attccgaatt
    18781 aacggaacat cgcgc
//
LOCUS       DASRUM010000014        24540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1680321, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000014 DASRUM010000000
VERSION     DASRUM010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 24540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24540
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680321"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="VFF22_00950"
     CDS             complement(<1..1374)
                     /locus_tag="VFF22_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZX15423.1"
                     /translation="MNHPLKALSTAVLLASLSTATQAMPYSQLIIFGDSLSDSGQFPD
                     VGSPLLGGNPTGGVRFTNRTGPNYLGNNTEYFDTVATQRLASLLGLQALPSTPILPQL
                     LTGNADGTNYAVGGYRTDQILSSITATNGSVVNAGGGISRTRNGYLVDVPRVDPDALF
                     YINGGGNDVLQFVVTDQVSAAAAAADLVAGVAALQAAGARTIIVSDLPDVGLTPAGFA
                     TGFRAAWSNASGLLNDELGTQLAALGGNVIRLNFRGLLTEVKADLASFGFDPLVAQTD
                     VCFSGSSCLADPTWGITGLTPNPDRLMFNDGVHPTAAVQQISADYIFSILGAPWEVSL
                     LPEMAMASLNAHQQQLRSEWQTDRGLWQAQGQWRSFVTASGMRQQFQRDAATSGGDSD
                     GLGVHLGGSYRFDENWRFGLSLGLQHQQLESDNDSEYDLRSYLLSAFSQYQKERLWAD
                     ASLSLGQL"
     gene            complement(1495..2049)
                     /locus_tag="VFF22_00955"
     CDS             complement(1495..2049)
                     /locus_tag="VFF22_00955"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZX15424.1"
                     /translation="MAWLQGLAFVLGTLMLLALSWRAVRSPQSHGFYRFFAWEAILAL
                     LVLNGPVWFVDRFALHQRLSWLLLFVSLLLLFAGLYQLRRMGRPDGQRKDVELFAFER
                     TSQLVTSGIFCLIRHPLYASLLLLAWGVACKQPNGLSLVLAMLASLALWLTAKRDEAE
                     CLQQFGEAYRDYMQRSKMFIPWVI"
     gene            2152..2559
                     /locus_tag="VFF22_00960"
     CDS             2152..2559
                     /locus_tag="VFF22_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hydroxyacyl-ACP dehydratase"
                     /protein_id="HZX15425.1"
                     /translation="MDRTQLHSLLPHQGPALWLDGLLAHDSLSIQGLSAWRFLETFGT
                     DASPCLLFEAAAQLCAAHGALYGDASAIEMALVGKLSQLQLHHEPTQRSGALQVSAQQ
                     EALSPAGALYAFSIHSEEQLLLDGKLLLVLIHA"
     gene            2552..3436
                     /locus_tag="VFF22_00965"
     CDS             2552..3436
                     /locus_tag="VFF22_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX15426.1"
                     /translation="MLELRRVGHCYPGTNRPALQGIDLQLQAGECLGLLGSNGAGKTT
                     LLSLLSGVLGLQQGELRWEGQPRLGLVPQQLAFYAGLKVSENLELFADLYRLRGSERR
                     QRLQQCIDICALEDKLARRSEQLSGGEQRRLNFAIGLLQPADLYLFDEATVGVDASSR
                     QQLLAAVEQLTADGKAVIYTSHYLEEVERVAQRILLLHEGQVRLDLDKRQLLGDDHGL
                     LLEWPDAPPTGLSELLAHLQLTTEVLPKGLRIASLNGQQLLAITEFVAAQDGLPNLLR
                     FGRPSLEQLYLRLNGGRL"
     gene            3433..4629
                     /locus_tag="VFF22_00970"
     CDS             3433..4629
                     /locus_tag="VFF22_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX15427.1"
                     /translation="MKLWAGLLALVRKEVLLLLRDPHALAVLFIMPTLFLLLMAGAMS
                     SYMQDKPPALRLVIEAPPDDASQFFREALQAQLPDSQLFDESSERLAHVRLPADFADK
                     LLDSPHQGPSLSFPAQFDKLSRQRLHSAVSIALAQTRLLAYLEDSGVVDAELSRAERL
                     QLVQQRTQSQIDEQEQLASGDLSGRANATQLSVPAWLIFGMFFVVLPMAGGFQREQQS
                     GTLLRLRCLGLNLGTLVLSKLLPYLAINLLQFVLLLGIGVYGLPLLGLPALSLPGSAA
                     AYPLLAISIALATSSLGLLLAALARSSEQALLLGGGINILLAAIGGIMVPKSVMPAAM
                     AQLAEISPMSWALDAFLTLLVGHGSLADIAPYCARLLLFAALCGIAGLLLFTRRVQRT
                     QWTTHY"
     gene            4611..4877
                     /locus_tag="VFF22_00975"
     CDS             4611..4877
                     /locus_tag="VFF22_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZX15428.1"
                     /translation="MDHSLLEQQLKQLLIRECDKEDDIDWQSIADDEPLFGAKSRIAM
                     DSLDALQVSLALQQHYGVRIEGAKDGRRILGSIASIAAYIRQQQ"
     gene            4874..5998
                     /locus_tag="VFF22_00980"
     CDS             4874..5998
                     /locus_tag="VFF22_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HZX15429.1"
                     /translation="MTPIYLQRAALHCALGDDLHSAAQALQSGQPPKGEPFVLHEVHE
                     PRNYLYATKTGESLSERLDRLLSETLGRNPQVLDDCLLIIASTSLDISELETLTSRQQ
                     GFRPDDSTSLDLLANQLRQRWGFAAAFTLNTACTSAANGLLYGARLIDAGQYARILVL
                     SFETPSTIAMQGFGALMLTSPSGQYRPFHPERDGLLLGEAYAAVLLSREPGDAPLARL
                     LGGFSACDTSSLTTTREDGSHIHWVMQQALRSAGCTAAQIDLVKLHGTATSANDEAES
                     NGMRLLYGEQMPALSLLKPWLGHTLGACGLSESLLLLQALQLGPLPAVDYASEAMLPL
                     SAEPQTLAADSLLLTNFFGFGGNNASLVLQGCSTGETLCR"
     gene            5989..6546
                     /locus_tag="VFF22_00985"
     CDS             5989..6546
                     /locus_tag="VFF22_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15430.1"
                     /translation="MPVIAASEIHGLAASSDKELKAALGQWLDIPPRRIDRLILQCLL
                     AAAPLKSQVRSNCGLYLASAHPARATMGALLEAVCVQHKQPKPFEFVNSVSNAAGFHI
                     AQQLGIDGPNLFIGAGGQVWAQLLSLANLDLQDGLIEQALLLVCDERGDFCVQAVLLE
                     GPTAGAYANSFAELSADAEVQRFSL"
     gene            complement(6625..7626)
                     /locus_tag="VFF22_00990"
     CDS             complement(6625..7626)
                     /locus_tag="VFF22_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HZX15431.1"
                     /translation="MSSFQHQQSAHCESGVMASLLTHAGLPMSEPMAFGLASGLAFAY
                     LPIVKLSGMPLIAYRMPPKHLIKTLSKRLGAKLHTRTFGNPEQGRRELDAALDSGRLA
                     GLQSSVFWLPYFPPEMRFHFNAHNLLAYGREGNEYLISDPVFEEPVRCAAEDLQKARF
                     AKGALAAKGLMYWLDDVPLEQDWNKLIRQSVLSTTRILDGMPLPWIGIRGIQHLAGRI
                     EKLDPAQAKYNRLYLTHIVRMQEEIGTGGAGFRFMYASFLQEAGEKLGDRNLQEASAQ
                     LTAIGDNWRQFASACVRASRNKGETPNFAPIAESLRGIALQERGLMKELAAWSKRKG"
     gene            complement(7623..8030)
                     /locus_tag="VFF22_00995"
     CDS             complement(7623..8030)
                     /locus_tag="VFF22_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15432.1"
                     /translation="MKLDEVPQDHSSTYGGHCKLVYAVDASGHYQGAQSDGWEPEAFA
                     TQLAVAELQEQAAEAEAGWRRGELSPLKCLMYHYRLDEPALAQITGLWQWRIRRHFRP
                     AIYRKLSAGILARYAEAFGLPVEQLISYQKGMA"
     gene            complement(8027..9193)
                     /locus_tag="VFF22_01000"
     CDS             complement(8027..9193)
                     /locus_tag="VFF22_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HZX15433.1"
                     /translation="MVQPVFINRISAFLPHEPVDNEQMEARLGQVGGKPSRARKLILR
                     SNGIQQRHYVIDPQTGAPSMSNAQVSAAAIRGLLGGDFQLEDLDCLVASSASPDQVMP
                     GHAVMVQGELGNPPCEVVSTAGICLCGMTALKYAWMSVASGESRNAVACGSEVASTLM
                     QASNFSAEYESRLDELETHPEIAFEKDFLRWMLSDGAGAVLLQSQPNRMGLSLRIDWI
                     DIFSFADQMPPCMYAGAEMVDGQLQGWSRYEGAERNRRSLMAIKQDVKLLNDNIVKYT
                     VEETLKRIMARRELHADSVDYFLPHYSSEFFRERLSVGLANAGLPIPQERWFTNLTSK
                     GNTGAASIFIMLDELFNSGSLRSGQRLLCYVPESGRFSSAFMQLTVVGQDGDNT"
     gene            complement(9213..10130)
                     /locus_tag="VFF22_01005"
     CDS             complement(9213..10130)
                     /locus_tag="VFF22_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dialkylresorcinol condensing enzyme"
                     /protein_id="HZX15434.1"
                     /translation="MRQVLIVHFSQTGQLDRLAQSVCAPLQQHGDIQLDFLQLQPAQP
                     YPFPWPFLEFFRIFPETVLMRPQPLLPLAVDASKRYDLVILAYQVWFLSPSQPMTAFL
                     ASPEAAQLLKGTPVVTLIGCRNMWLMAQEKVKSRLTELGARLVDNIALTDACGTAASF
                     LATPLWLFTGRQKPYSWVPRAGIDEAEIVAASRFGQAMAQRLAADSLPIEQPMLTGLG
                     AVRIDEKLIASEKVGNRSFQLWSRLLAALGPQQSRRRGAGLVVYILFLLCLIVTVVPL
                     SALLKKLLAPLLKERTQREKAYFAGPSGE"
     gene            10216..10608
                     /locus_tag="VFF22_01010"
     CDS             10216..10608
                     /locus_tag="VFF22_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HZX15435.1"
                     /translation="MLLLVGSSAQAGDILITVDGIQTTGTVHAALVSGEQKRWPEQPL
                     RQQQSDDGQLRLIGVPSGRYAIQLYQDRNGNGQLDQGRRGIPLEPVGFSASPPLLNGK
                     PTPADSLFEHRTTDTHLRIRLQSPKAKK"
     gene            complement(10622..11179)
                     /locus_tag="VFF22_01015"
     CDS             complement(10622..11179)
                     /locus_tag="VFF22_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019411731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HZX15436.1"
                     /translation="MRLLAALAAAAVLVGCANSGTGVVPPKAVEEVDLQRYQGTWYEQ
                     ARLPMFFQRNCAQSEALYELQEGGTIGVTNRCRTYDGELQQVQGQAVAQVPGETSKLW
                     VRFDNWASSLLPGLTKGEYWVLYLDDDYQSALVGHPNRKYLWLLTRDQQLSAQNRATL
                     LEEARSRGYDTDELIWRSTDAEMAR"
     gene            complement(11179..11433)
                     /gene="bamE"
                     /locus_tag="VFF22_01020"
     CDS             complement(11179..11433)
                     /gene="bamE"
                     /locus_tag="VFF22_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003171143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HZX15437.1"
                     /translation="MSLRVVALLAGCLLLAACNKINQDNYTQLKAGMSKDEVESLLGK
                     PGECAGALGMSSCTWGDEQRFISVQYAGDKVMMFSGKGLK"
     gene            complement(11502..12119)
                     /locus_tag="VFF22_01025"
     CDS             complement(11502..12119)
                     /locus_tag="VFF22_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HZX15438.1"
                     /translation="MSAPWQPLLDWWFGAAGCGSGVTAAEVAASRTALWFGKQDRQDD
                     QAREQFGALVEQALAGQLQDWQGEPEGWLATLLLLDQLPRMIFRDTPRAFAGDALARP
                     LALHGLTQGWDQQLPPIQRVFTYLVLEHAEDLALQDRAVRLFQVLREQAVATERELFA
                     GYLDYAERHQRVVARFGRFPHRNQILGRTSSVEESAFLLEPGSRF"
     gene            complement(12123..13130)
                     /locus_tag="VFF22_01030"
     CDS             complement(12123..13130)
                     /locus_tag="VFF22_01030"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HZX15439.1"
                     /translation="MSRVTLSRYLIEQTRSNNTPADLRFLIEVVARACKEISHQVSKG
                     ALGGVLGSMGTENVQGEVQKKLDVISNEILLEANEWGGHLAGMASEEMDNAYQIPGKY
                     PKGAYLLVFDPLDGSSNIDVNVSVGTIFSVLRCPDRNGNEGDLGEEAFLQPGTEQVAA
                     GYAIYGPQTMLMLTLGDGVKGFTLDRELGSFVLTHDNIKVPESTKEFAINMSNQRHWE
                     APVQRYVSELLAGEEGPLGKNYNMRWVAAMVADVHRILTRGGVFMYPRDSREPEKPGK
                     LRLMYEANPMSMIIEQAGGVATNGTQRILDIQPTSLHQRVAVFLGSKEEVLRISAYHR
                     E"
     gene            complement(13220..14863)
                     /locus_tag="VFF22_01035"
     CDS             complement(13220..14863)
                     /locus_tag="VFF22_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HZX15440.1"
                     /translation="MNACGDSAWALSAPVILTSVVCVGVLLLARWVTRQRYFPGRESF
                     IVLHLASLWWLIAASLEMAAQGPQCKVFWATIAWPGILTTPTFWAIFLWQYVNSVRQP
                     LPLRSTLSLALVPLLIWLMALSNPWHGLFYGAGTAPLSDEPGAPIRYQHGPLFYAAAA
                     YVYLFMTFCMGVVLRAALVSYGVHRRHYLAFVFVTVVPWVANISYVVFGWMLFGFDPT
                     PFSFAFTLVAFAWLIVGVRLFDLLPVARHLLLDALLDPVLVVDAQLRVIEANPAALKL
                     AGLRSGWQGRQLADWPILGADLQQLLSQQTQGEHDRLLTLANSARYFEVRIRAIERVT
                     HRGTLVLGQMLYLRDVTQRHLSELKLAEALALSEERLRTITNLHEQLQEQALCDPLTG
                     LYNRRYLDEFFARELAVAQRERTPISLALIDLDHFKQLNDEHGHLEGDDVLKGVAQHL
                     LENLRSSDAVFRIGGEEFLLILPRADAPEARARLETICRELAAHPLPTRGGARFVTLS
                     AGLALWPEQGLVLDELLQVADAALYQAKHAGRNRVCSLA"
     gene            complement(15058..16425)
                     /locus_tag="VFF22_01040"
     CDS             complement(15058..16425)
                     /locus_tag="VFF22_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 domain-containing protein"
                     /protein_id="HZX15441.1"
                     /translation="MISRLIHRVGGVALLGLLCSAPALAQEQPGDYAMQLPLSPSGEG
                     PWYSLDVPMALHFAARYGDLRDLRVFNAEGQALAYALVAGQGESRDSQQEQAVKWFPL
                     YAEQGDSNATPSLRVQRSTSGTLIELLEESDAAAKPAQLRGWLLDASALDEPLVRLQL
                     DWSGGQEGFQRFSIEASDDLQDWQPWGSGQLARLSFDGERVEQRQIELPGNKTRYLRL
                     LWQNPQQAPQLTAASLRSQRSDSVPAPLVWSQPLAATRSPDGHYQWQMPLALPLERVH
                     VELSQANSLAPIRVTGRSEAKGAWQSLAQGLLYRLPENGVEIRQDELPLPGWAVQQLR
                     LQVDERGGGLGVEPPQIKVAVRATQLVFLQRGSPPYRLALGRAGAKSAALPLTTLIPG
                     YQPQRLQQLGTAEAQLQIEGLAQQEQAVGESDWKRWGLWLVLLLGVGVLALMAVSLLR
                     RPSAE"
     gene            complement(16422..20027)
                     /locus_tag="VFF22_01045"
     CDS             complement(16422..20027)
                     /locus_tag="VFF22_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HZX15442.1"
                     /translation="MQWIFMLVGLVLGALADETLTASLLGALIGLGLGQALKLHGLER
                     ENAALTAQLKAFSQRFEQGTAALYERLLKLEQSAMPAPEAPPTPQPVNSVVEPVPEPI
                     VSAQAAQPELDWTLDFVLPEPAPEPVAPMQATAEPAAAPIKPPPATPWKPAEPRQPTL
                     LERGFTAAKDWLLGGNTVLRVGVVLLFLGLAFLLRYATEGVVVPVELRYASVAASAVA
                     LLALGWWLRLRNPNYALMLQGTGIAVLYLTVFAAMRLHPLLDPGVALGLLVLVTVFSA
                     ILAVKQNALGLAAVAALGGFAAPILTSTGSGNHVALFSYFALLNAGIFAIAWFKAWRL
                     LNLIGFVGTFGIGFAWGIRSYTPELLWSTEPFLILFFLMYVAIGLLFARRKLREALDA
                     PEDRDALLRWAARKGDYIDASVLFGPPLVGFGLQFALVQHIEFAAAFSALALGLFYIV
                     LARLLVGRTGDRAVLLVETCLALGVVFGSLAIPLGLDARWTSAAWAVEGAGIFWLGLR
                     QQRSLARAFALLLQFGAALAFAIGLRAGELTLLDGAPLGALMLGVALLFSYWQLRQNT
                     QHASSWEVRGLPVLACAGLAFLYLIAPLLFAAQGTAITWALAGLLTLWLGLRLQARSF
                     LFSAFAVQLLGGLVFMLGMAGNAATGMGGFSAGWQGLLTSSLIGFGLIAGMLLAARDP
                     QVGADRRLLRGLSVVLLVGLVFLNLAVLFVLPWATASAVWGGSGLLIIWLSLHLQQRA
                     SFVFGLLLQVLAGLVFLAVSPLLFGQLSAEGLRPLAHVDFWTPLVLGVAALVGAWRLQ
                     QLARREAPSALGNFSLLGLAQLLLVWGAAWWALALSSEVLRFVAVEWQSSALLAVLAA
                     SVALAAFLAPRTRWAELALLCCLLTPLAGVVLLHAWHLHYHPAAQFGWLAWTLLFAVH
                     FWALRRLAAQLPAGALSAAHVLGCWLLLGVLALELRYLLLALSEHYNAWRWLGWALLP
                     SAYLWLMAPWQMTPSRRWPWPLAAYPREYRLLAAAPLALLMLGWFWLANVVSAGDAEP
                     LAYLPLLNPLELGLLFALGAVFAWARMGLAELGVDSVRSQWLTQAVTGASLFALLTAM
                     VMRTAHHWGGVPYQLDALLDSMLVQAGLSIVWTLIALPLMVLGNRSGRRELWLIGAAL
                     IALVVAKLFFVELGNRGGLERIVSFIGVGVLLLVVGYFAPLPARKADAQQAQEQA"
     gene            20272..20667
                     /locus_tag="VFF22_01050"
     CDS             20272..20667
                     /locus_tag="VFF22_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZX15443.1"
                     /translation="MKNNLIAAAELDRLETWAKYTADMCHSCMSSCCTLPVEARLNDL
                     IRIGVVDEFERSEPLKNIAKRLQKDGIVERFHQKSGIFTLTRMSNNDCYYLDRKTRLC
                     TIYDKRPDTCRNHPKIGPRPGYCAYKPKA"
     gene            20729..22135
                     /locus_tag="VFF22_01055"
     CDS             20729..22135
                     /locus_tag="VFF22_01055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019372517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZX15444.1"
                     /translation="MSPWRSLSLWMDQLDEPLTARPALTEDLHADVAIIGAGYTGLWT
                     AYYLKRQAPQLRIVILEDEIAGFGASGRNGGWLMGNLLGQDRLLADQPSAERQSAYAL
                     LHGIPDEVASVLQREQIACDYRKAGVLYCAARYPEQLTSLRQQLAELRALGLDENDYR
                     WLTPFDLGKQLQLANAYGAIYTPHCATIQPAKLVRGLARCVAAMGVELYEQSRVIDWQ
                     TGLVRTASGSVRADWIVPAVEGYAACLPPLGKHQIPVQSLLVATEPLPKEVWADIGLE
                     RGQAFSENSRQVTYGQRSADDRLVFGARGGYRFGGQLRTDFNLSENERDLRRDLFGEL
                     FPQLKQVRITHAWGGNLGMARRFRPHMLVDRKQGIALSGGYGGEGVGASNLGGRTLAD
                     LILDQQSELTRQPWVLGDQPINRLPRWEPEPLRWLGYNAIIQSFALEDRVLANPDSAP
                     WRRKLASSLAGTLEKLMR"
     gene            complement(22146..22265)
                     /locus_tag="VFF22_01060"
                     /pseudo
     CDS             complement(22146..22265)
                     /locus_tag="VFF22_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677569.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            22423..23487
                     /locus_tag="VFF22_01065"
     CDS             22423..23487
                     /locus_tag="VFF22_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZX15445.1"
                     /translation="MQIPNVEQKAFILLLVLVTVAFFWILLPFYGAVFWAMVLAVVFT
                     PLQRRMARRIGGRGNLSALLTLVVCLLVAILPVIFITSAVVAEGASLYQKLESGELDI
                     GSYVTSTIRALPPTLQEQLDRIGVSDLDGLREQISTVAMAGSQFLATKVFAIGQGTFA
                     FFVSFFVMLYLLFFLLRDGLELARTIRETIPLGESTKRRLQIKFTRVVRATVKGNVVV
                     AAVQGALGGLIFWALGIYSPLLWGVLMAFLSLLPAVGAGIIWLPVAVYLILSGSLWQG
                     VILTVFGVLVIGLVDNILRPILVGKDTRMPDYLILISTLGGLALLGLNGFVIGPLVAA
                     LFLSSWNLFEGGKRTVKLPD"
     gene            complement(23607..>24540)
                     /locus_tag="VFF22_01070"
     CDS             complement(23607..>24540)
                     /locus_tag="VFF22_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007939427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HZX15446.1"
                     /translation="MTPLTVDEPTVSMTFQVNDSPFCGKEGKFVTSRNIKERLDKELL
                     YNVALRVEEGDSADKFKVSGRGELHLSVLIETMRREGFEMGVGRPEVIIRTVDGVKQE
                     PFENVTIDTPEESQGKVMEEMGLRKGDLTNMVPDGKGRVRLEYNIPARGLIGFRNQFL
                     TLTNGAGILTSIFDRYDTMKSGDMSGRQNGVLVSVETGKALTYSLETLQARGKLFVEH
                     GQEIYNGQIVGLNSRDNDMGVNPTKGKKLDNMRASGKDETIALVPPVKFTLEQALEFI
                     QDDELCEVTPKSIRLRKKILDEGERTRAAKKAKN"
BASE COUNT         5060 a         8100 c         7199 g         4181 t
ORIGIN      
        1 cagctgcccc agactcaggc tggcatcagc ccacagacgc tccttttggt actgggaaaa
       61 ggcgctgagc aaatagctgc gcaggtcgta ctcggaatcg ttgtcgctct ccaattgctg
      121 atgctgtaag cccagcgaga ggccgaagcg ccagttttca tcgaagcggt aactaccgcc
      181 caggtggacg cccaagccat cactgtcgcc accactcgtg gcagcatcac gctgaaactg
      241 ctgacgcatg ccgctcgccg tgacaaaact acgccactgg ccctgagcct gccaaaggcc
      301 acgatccgtc tgccactcac tgcgcaattg ctgctgatgg gcattcaggc tggccatcgc
      361 catttccggc agcagggaca cttcccaggg cgcgcccagt atcgaaaaaa tataatccgc
      421 actgatctgt tgcacggctg cagtcgggtg caccccgtca ttgaacatca agcggtcagg
      481 gttgggcgtg agccctgtga tgccccaggt cggatcggcc aggcaactac tgccgctgaa
      541 gcagacatcc gtctgcgcca ccaaggggtc gaagccgaaa gaggccagat cagccttcac
      601 ctcggtaagc aacccacgga aattcaggcg aatgacgttg ccgcccaatg cagccaactg
      661 agtacccagc tcatcgttga gcagccctga tgcattcgac caggccgcgc ggaagccggt
      721 ggcgaaaccc gcaggcgtca agccgacgtc cgggagatcg gagacgataa tggtgcgtgc
      781 accagcagcc tgcagagccg ccacccctgc gaccaaatcg gctgccgctg ccgcagcgct
      841 cacctggtcc gtcacaacaa actgcagcac atcgttgccg ccaccgttga tgtagaacaa
      901 cgcatcgggg tcaacacgtg gcacatccac cagatacccg ttacgggtac ggctgatgcc
      961 gccgccagca ttcaccaccg aaccattggt cgcggtgatc gaactcagga tctggtcggt
     1021 acggtagccg cccacggcat agttggtgcc atcggcgttg ccggtcagta actgcggcag
     1081 aatgggtgtc gagggcaacg cctgcaggcc gagtagggag gccaagcgct gggtggcaac
     1141 cgtatcgaag tactcggtgt tgttgcctag gtagttcggc ccagtgcggt tggtaaaacg
     1201 caccccacca gtcgggttcc cacccagtag agggctgccc acatctggaa actgcccact
     1261 atcgctcaag gaatcgccaa agatgatcaa ctgcgagtag ggcatggctt gtgtcgctgt
     1321 agacaggctc gccagcagca cggcagtgct caaggctttc aatggatgat tcatgggatt
     1381 ccccttctta ttgtgatgcc cactacaggc aagcgaagga gactaatgag aacccgcgca
     1441 cacgaccctc acccaaaagg gtgaatcgaa agcctattca gcggttgaat agtgctatat
     1501 cacccaaggg ataaacattt tgctgcgctg catatagtcc ctatacgcct cgccaaactg
     1561 ctgcaggcac tcagcctcat cgcgcttggc ggtcagccac agcgccaggc tggctagcat
     1621 tgccaggacc aggctcaaac cattgggctg cttgcaggcc acgccccagg ccagcaacag
     1681 cagcgaggcg tacagcgggt ggcggatcag gcagaagatg ccgctagtca ccagctgcga
     1741 tgtgcgctca aaggcaaata gctcgacatc cttacgctgc ccgtcgggac gacccatacg
     1801 ccgtagctga tagaggccgg caaacagcag cagcaaggac acaaacaaca gcagccagga
     1861 caggcgttga tgcagggcaa aacggtcgac aaaccagacc ggcccgttga gcaccagcaa
     1921 ggccagaatc gcctcccagg cgaagaaccg gtagaaacca tgggactgcg gactgcgcac
     1981 tgctcgccag gacagcgcca gcagcatcag cgtgcccagc acaaaagcca agccttgcaa
     2041 ccacgccata aagccttccc ccactccgct cgatagtccg tcaaagccag gcgctgcgtg
     2101 acttgcagcc cctctgttac actgccggcc tctttgcctc ggccgcccgc aatggatcgc
     2161 acccaactcc acagcctgct gccccaccaa ggcccagccc tctggctgga tggcctgctg
     2221 gcgcatgaca gcctgagtat tcagggcctg agtgcctggc gctttctcga gacatttggc
     2281 accgacgcct caccctgcct gctgttcgaa gcagcggccc aattgtgcgc cgcgcatggt
     2341 gccttatacg gcgatgccag tgccattgaa atggccctgg ttggcaagct ctcgcagttg
     2401 cagctgcacc atgaaccgac ccagcgcagt ggcgcgctgc aggtcagtgc ccagcaggag
     2461 gcgctaagcc cggccggcgc gctgtatgcc ttcagtatcc acagcgaaga acaactgctg
     2521 ctcgacggca aactgctgct ggtgttgatc catgcttgaa ctccgccgcg tcggccactg
     2581 ctacccaggc accaaccgcc ccgcactgca gggcatcgat ttgcagctgc aggccggcga
     2641 atgtctgggg ctgctcggca gcaacggcgc aggcaaaacc accctgctct ccttgctgag
     2701 cggcgtgctc ggcttgcaac agggcgagct gcgctgggag ggccagccca gactcggcct
     2761 ggtgccgcag cagctggctt tctatgccgg actcaaggta agtgagaacc ttgaactgtt
     2821 tgccgacctg tatcgcctgc gcggcagcga acgccggcaa cgcctgcagc aatgcatcga
     2881 tatctgcgcc ctagaagaca agctggcgcg gcgtagcgaa caactctccg gcggtgaaca
     2941 acggcggctg aatttcgcca tcggcctgct gcaaccggct gacctctatc tattcgatga
     3001 ggccaccgtt ggtgtggatg ccagtagccg ccaacaactg ctggccgccg tcgagcagct
     3061 gactgccgac ggcaaggcgg tgatctacac cagtcattac ctggaagaag tcgagcgggt
     3121 ggcgcagcgc attctgctgc tgcacgaagg ccaggtgcgc ctggatctgg acaaacgcca
     3181 gctgctcggc gacgaccacg gcctgctgct ggaatggccg gacgcgccgc caaccggcct
     3241 gagcgaactg ctcgcacacc tgcaactgac caccgaagtg ctgcccaagg gcctgcgtat
     3301 tgcatccctg aatggccagc agctgctggc catcaccgaa tttgtcgccg cccaggatgg
     3361 cctgcccaac ctgctgcgct tcggccgccc ttcgctggag cagctctacc tacgccttaa
     3421 cggaggccgg ctatgaagct ctgggcaggg ctcttggcat tggtgcgcaa ggaagtgctg
     3481 ttgctgctgc gcgaccccca tgcgctggcc gtgctgttta ttatgccgac cctgtttctg
     3541 ctgctgatgg ccggggccat gtccagctat atgcaggaca aaccgccggc gctgcgcctg
     3601 gttatcgaag cgccgcccga cgacgccagc cagttcttcc gcgaagcctt gcaggcgcaa
     3661 ctgccggaca gccagctgtt cgacgaaagc agcgaacgcc tggcccatgt gcgcctgccc
     3721 gcagacttcg ccgataaatt actggacagc ccgcaccagg gccccagcct gagtttcccg
     3781 gcgcaattcg acaaactctc gcgccagcgc ctacacagcg cggtgagcat cgccctggcg
     3841 cagacccgac tgctggccta cctggaagac agcggtgtgg tcgatgcaga actaagccgt
     3901 gccgagcgtc tgcaactggt gcagcaacgt actcagagcc agatcgatga gcaggagcaa
     3961 ctagccagcg gcgaccttag cggccgcgcc aacgccaccc aactgagcgt gccggcatgg
     4021 ctgatcttcg gcatgttctt cgtcgtgctg cccatggccg gcggctttca gcgcgaacag
     4081 cagagcggca ccctgttgcg cctgcgctgc ctagggctga acctcggcac cctggtgcta
     4141 agcaagctgc tgccgtacct ggcgatcaat ctgctgcagt tcgttctatt gctgggcatc
     4201 ggcgtctacg gcctaccgct gcttggcctg cccgccctaa gtttgcccgg cagcgccgcg
     4261 gcctatccgc tattagccat cagcatcgcc ctggccacca gcagcctggg cctgttactg
     4321 gccgcattgg cccgcagcag cgaacaggcg ctgctgctcg gcggcggcat caacatcctc
     4381 ctagctgcga ttggcgggat catggtgccg aaaagcgtaa tgccggcggc catggcccag
     4441 ttggcagaaa tctccccgat gagctgggcg ctcgacgcct ttctcaccct gctggtgggc
     4501 cacggctcac tggccgacat cgccccctat tgcgcgcgcc tgctgctgtt tgcagcgctg
     4561 tgcggcatag ccgggctgct tctgttcacc cgacgagtac agcgcacgca atggaccact
     4621 cactactaga acaacaactc aaacaattgc tgatccgcga gtgcgacaag gaagacgata
     4681 tcgactggca gagcatcgcc gacgacgagc cgctattcgg cgccaagagc cgtatcgcca
     4741 tggacagcct ggacgccctg caagtctccc tggccctgca gcagcactac ggcgtacgca
     4801 ttgagggcgc caaggatggc cggcgtatcc tcggtagcat cgccagcatc gccgcctata
     4861 tcaggcagca gcagtgaccc cgatctacct gcaacgcgcc gccctacact gcgccctcgg
     4921 tgatgacctg cacagcgccg cccaggccct gcaaagcggc cagccgccaa agggcgagcc
     4981 ctttgtgctg catgaagtgc atgagccacg caattatctg tacgccacga aaaccggcga
     5041 aagcctgagc gagcgcctgg atcgcctgct gagcgaaacc ctcggcagaa atccccaggt
     5101 actcgacgac tgcctgctga tcatcgccag caccagcctg gatatcagcg aactggaaac
     5161 tctgaccagc cggcagcagg gctttcgccc ggacgattca acgtccctcg acctgctcgc
     5221 caaccagctg cgtcagcgct ggggctttgc cgccgccttt accctgaaca ccgcctgcac
     5281 cagcgccgcc aacggcctgc tttatggcgc ccggctgatc gacgcgggcc aatacgcccg
     5341 catcctggtg ctgagtttcg aaacgcccag caccatcgcc atgcagggtt ttggcgcgct
     5401 gatgctgacc agccccagcg gccagtaccg gcccttccac cccgaacgcg acggcctgct
     5461 gctgggcgaa gcctacgctg cggtgctgct cagccgcgag ccgggtgacg caccactggc
     5521 acgcctgctc ggcggcttta gcgcctgcga caccagcagc ctgaccacca cccgcgaaga
     5581 tggcagccat atccactggg tgatgcaaca ggcgctgcgc agcgccggct gcacggcggc
     5641 gcagatcgac ctggtgaaac tgcacggcac cgcgaccagc gccaacgatg aagccgaaag
     5701 caacggcatg cgcctgctct acggcgaaca gatgccggcc ttgagcctgc tcaaaccctg
     5761 gctcggccat accctgggtg cctgtgggct aagtgaaagc ctgctgctgc tccaggcact
     5821 gcaactgggc ccgctaccgg cagtggatta cgccagcgaa gccatgctgc ccttgagtgc
     5881 agagccacag accctggcgg cagacagcct gctgctaacc aacttcttcg gcttcggcgg
     5941 caataacgcc agcctggttc tgcaaggctg cagcacggga gagaccttat gccggtaatc
     6001 gccgccagcg aaatccacgg ccttgccgcc agtagcgata aagagttgaa agccgctctg
     6061 ggccagtggc tggacatccc accccggcgc atcgaccggc tgatcctgca atgcctgttg
     6121 gccgccgcac cactgaaaag ccaggtacgc agcaactgcg gcctgtacct ggcttccgcc
     6181 cacccggcgc gggccaccat gggcgcactg ctggaggcgg tgtgcgtgca gcacaagcag
     6241 cccaagcctt tcgagtttgt gaatagcgta agcaacgccg cgggctttca tatcgctcag
     6301 cagctgggca tcgatgggcc gaatctgttt atcggcgcag gggggcaggt ctgggcacag
     6361 ttgctcagtc tggccaatct ggatttgcag gatggtttga tcgaacaggc actattgctg
     6421 gtctgcgatg agcgcgggga tttttgtgtg caagccgtgc tgctggaagg gccaactgct
     6481 ggcgcgtacg ccaacagttt tgccgaatta agtgcagatg ccgaggtgca gcggttcagc
     6541 ttgtagggtg cgccgcgcgc acctgatggc atcgcaccca tcttgaggtg cgcacagcgc
     6601 accctacaac ttcggcgacc gacctcagcc cttgcgcttg ctccaggccg ccagctcttt
     6661 catcaacccg cgctcctgca gggcaatccc gcgcaggctt tcggcgatgg gcgcgaagtt
     6721 tggtgtctcg cccttgttgc ggctggcgcg cacgcaggcc gaggcgaact gacgccagtt
     6781 gtcaccgatg gcggtaagtt gtgccgaggc ttcctgcagg ttgcggtcgc ccagtttctc
     6841 gcccgcctcc tgcagaaagc tcgcatacat aaagcggaaa cccgcgccgc cggtgccgat
     6901 ctcttcctgc atacgcacga tatgggtcag gtacaggcgg ttgtacttgg cctgggccgg
     6961 gtcgagtttt tcgatccgcc ccgccagatg ctggatgccg cgaataccga tccatggcag
     7021 cggcatgccg tcgagaatgc gcgtggtcga cagcacgctc tggcggatca gcttgttcca
     7081 gtcctgctcc agtggcacat cgtcgagcca gtacatcagc cccttagcgg ccagcgcacc
     7141 tttggcgaag cgggcctttt gcaggtcttc ggcagcgcag cgcaccggtt cttcgaatac
     7201 cggatcgctg atcaggtatt cattgccctc gcggccatag gccagcagat tgtgcgcatt
     7261 gaagtgaaaa cgcatctccg gcggaaagta cggcaaccag aacaccgagc tctgcagccc
     7321 ggccagccgt ccgctgtcca gcgccgcatc cagctcacga cggccctgtt cgggattgcc
     7381 aaaggtgcgt gtgtgcagct tcgcgcccag gcgcttgctc aaggtcttga tcaagtgctt
     7441 gggcggcatg cggtaggcaa tcagcggcat gccgctgagc ttgacgatcg gcagataggc
     7501 aaatgccaaa cccgaagcca ggccgaaggc catcggctcg ctcatcggca ggccggcatg
     7561 ggtcaacaga ctggccatca ccccgctttc gcagtgggcg ctctgttgat gctgaaaact
     7621 gctcatgcca tgcccttctg ataggagatc aattgctcaa cgggcaggcc gaacgcctcg
     7681 gcgtatcgcg ccagaatgcc tgccgacagc ttccggtaaa tcgccgggcg aaagtgcctg
     7741 cggatgcgcc attgccacag cccggtaatc tgcgcgagcg ccggctcatc caggcgatag
     7801 tggtacatca ggcacttcag cggcgacagc tctccgcgcc gccagcctgc ttcggcctcg
     7861 gcggcctgtt cctgcagctc ggctacagcc agttgggtgg cgaaggcttc cggctcccag
     7921 ccatcgctct gcgcgccctg ataatggcca ctggcgtcca cggcgtagac cagtttgcag
     7981 tggccgccgt aagtggagct gtgatcctgg ggaacctcat caagcttcat gtgttgtcac
     8041 cgtcctggcc aaccactgtc aactgcataa aggcgctgga gaaacgccca ctttccggca
     8101 catagcagag caaacgctgg ccgctgcgca ggctacctga attgaacaac tcatcaagca
     8161 tgataaagat cgacgcggcg ccggtattgc ctttgctggt caggttggta aaccagcgct
     8221 cttgcggaat cggcaaaccc gcattggcca ggcccacgga cagacgctcg cggaagaact
     8281 cggacgaata gtgcgggagg aaataatcga ccgagtcggc atgtaactca cgccgcgcca
     8341 tgatgcgctt cagagtctcc tccacggtgt atttgacgat attgtcgttc agcagtttga
     8401 cgtcctgttt gatcgccatt agtgaacgcc gattgcgctc agcgccttca tagcggctcc
     8461 agccctgcag ctggccgtcg accatttccg ccccggcgta catgcacggc ggcatctggt
     8521 cggcgaagga gaagatatcg atccagtcga tacgcaggct caggcccatg cggttcggct
     8581 ggctctgtag cagcacagca ccggcgccat cggagagcat ccagcgcaga aaatccttct
     8641 cgaaggcgat ttccgggtgg gtttccagct cgtcgaggcg gctctcgtac tcggcgctga
     8701 agttgctcgc ctgcatcagg gtcgaggcca cttccgagcc acaggccacg gcgttgcggc
     8761 tttcgccact ggccacgctc atccaggcgt atttcagcgc ggtcatgccg cacaggcaga
     8821 taccggcggt ggagaccact tcgcacggcg gattgcccag ctcgccttgc accatcaccg
     8881 catggccagg catcacctga tccggcgagg cactgctggc caccaggcaa tcgagatctt
     8941 ccagctggaa atcgccaccg agcaggccgc gaatcgccgc ggcgctgacc tgggcattgc
     9001 tcatgctcgg tgcgccagtc tgcgggtcga tcacatagtg acgctgctga atgccattgc
     9061 tacgcagaat cagcttgcgc gcccgcgacg gcttgccgcc gacctggccc aggcgcgctt
     9121 ccatctgctc attgtcgacc ggctcatggg gcaggaaggc gctgatgcga ttgataaata
     9181 caggttgtac cacggggttt cctcgggtca cttcactcgc cagacgggcc ggcaaagtaa
     9241 gccttctcac gctgggtgcg ctcttttaac agcggcgcca acaacttttt aagcaaggca
     9301 ctgagcggca ctacggtgac gatcagacac aacagaaaca ggatatacac caccagcccg
     9361 gcgccgcgtc ggcggctctg ctgcgggcct aaagcggcca gcaagcggct ccataactgg
     9421 aagctacgat taccaacctt ctcgctggcg atcagctttt catcaattcg cactgcgcca
     9481 agcccggtca gcatcggttg ctcaatcggc aggctgtcag ccgccagccg ctgcgccata
     9541 gcctggccaa agcggctggc ggcgacaatt tctgcttcat caataccggc gcgcggcacc
     9601 cagctgtaag gtttctgccg tccggtaaac agccacaagg gtgtcgccag aaaactcgcg
     9661 gcggtgccgc aggcatcggt cagggcgatg ttgtccacca gccgcgcgcc cagttcagtg
     9721 agccggctct tgaccttttc ctgggccatc agccacatat tgcggcagcc aatcagggtc
     9781 accactggcg tacccttgag cagctgcgcc gcttcaggcg aggcgagaaa ggcggtcatc
     9841 ggctgcgacg gtgaaaggaa ccacacctga taggccagga tcaccaggtc atagcgcttg
     9901 ctcgcatcca ccgccaacgg cagcagaggc tgcgggcgca tcaatacggt ttctgggaat
     9961 atccggaaaa actcgagaaa cggccaggga aaggggtaag gttgcgcggg ttgcagctgg
    10021 agaaaatcca gctgaatatc gccgtgctgc tgcagcgggg cacagaccga ctgcgccaac
    10081 ctgtctagct ggccggtttg cgaaaaatga acgatcaaga cctgacgcat cgccagccac
    10141 ttcctgcgaa aggcccggat tatgccacag agaactcttc aaactgtaaa gaaggccggc
    10201 tatcaagctg gccttttgct gctactggtc ggctcaagcg cacaggccgg cgacattctg
    10261 atcacagtgg acggcatcca gaccacggga accgtgcatg cagcgctggt gagcggcgag
    10321 cagaaacgct ggccagaaca accgttgcgt cagcagcaga gtgacgacgg ccagctacgc
    10381 ctgattggcg tgccgtccgg gcgctacgcc attcagctgt atcaggaccg caatggcaac
    10441 ggccaactcg accagggccg acgcggcatc ccactggaac cagtgggttt ttccgccagc
    10501 ccaccgctgc tcaatggcaa acccacaccg gctgacagcc tgttcgagca ccgcacgacg
    10561 gacactcatc tgcgcatccg cctgcagagc ccgaaagcca agaaataagc agcccttacg
    10621 gttaccgcgc catctcggca tccgtgctgc gccagatcag ctcgtcagtg tcatagccac
    10681 ggctgcgcgc ttcttcaagc aaggtcgcgc ggttttgcgc gctgagctgc tgatcacggg
    10741 tcagcaacca gaggtacttg cggttgggat gaccgaccag cgcgctctgg tagtcgtcat
    10801 ccagataaag cacccaatac tcgcccttgg tcagccccgg cagcaggctg ctggcccagt
    10861 tgtcgaagcg tacccagagc ttgctggtct cgcccggcac ctgagccacg gcctggccct
    10921 gcacctgctg caactcgcca tcgtatgtgc ggcagcgatt ggtcacgcca atagtgccgc
    10981 cttcctgcaa ctcataaagc gcctcggact gcgcgcagtt gcgctgaaag aacatcggca
    11041 aacgcgcctg ttcgtaccag gtgccttggt agcgctgcag atcgacttcc tccaccgcct
    11101 tcggtggcac tacgcccgta ccggagttgg cacaacccac cagcacagcc gctgcggcca
    11161 acgccgccag caggcgcatc acttcaagcc cttgccggaa aacatcatga ccttgtcgcc
    11221 ggcgtactga acgctgataa agcgctgttc atcgccccag gtgcagctgg acatgcccag
    11281 cgcgccggcg cactcaccag gcttgccgag caggctctcg acttcatcct tgctcatgcc
    11341 cgccttgagc tgggtgtaat tgtcctgatt gatcttgttg caggcagcca gcaacaggca
    11401 accagcgagt agtgcgacga cacgcagaga catggggaaa ctcctgagga aagaaccaac
    11461 accagcttgg catagcaata ccgagcgtgc ctgcttcgac gctagaagcg cgagccaggt
    11521 tccagcagaa aggcagattc ctcgacgcta gaagtgcgcc cgagaatctg attgcgatgg
    11581 gggaaacgac caaagcgtgc gaccacccgc tgatggcgct cggcatagtc cagataaccg
    11641 gcaaacagct cgcgctcggt ggctacggcc tgctcgcgca acacctgaaa cagccgcacc
    11701 gcccgatcct gtagcgccag gtcttcggca tgctccagca ccagataggt aaatacccgc
    11761 tggattggcg gcagttgctg atcccagccc tgggtcagcc cgtgcagggc caagggtcga
    11821 gccaacgcat caccggcaaa ggcgcgcggg gtatcgcgaa agatcatccg gggcagctga
    11881 tcgagcaaca gcagcgtcgc cagccagcct tcaggctcgc cttgccagtc ttgcaactga
    11941 ccggccaggg cctgctcgac cagcgcgcca aactgctcgc gcgcctgatc atcctggcga
    12001 tcctgcttgc cgaaccacag cgccgtccta ctggccgcta cctcggcagc cgtcacaccg
    12061 gaaccgcagc cggccgcacc gaaccaccag tcgagcaacg gctgccaggg cgcgctcatc
    12121 acttactcgc ggtgataggc gctgatgcgc agcacttctt ccttggaacc gaggaagacc
    12181 gcaacacgct ggtgcaggga agtcggctgg atatcgagaa tgcgctgggt gccgttggtg
    12241 gccacgccgc cggcctgctc gataatcatc gacatcggat tggcttcgta catcaggcgc
    12301 agcttgcccg gcttctccgg ctctcggctg tcgcgcgggt acataaacac gccaccacgg
    12361 gtgaggatgc gatgcacgtc ggcaaccatc gcggcgaccc aacgcatgtt gtagttcttg
    12421 cctagcggcc cctcttcgcc agccagcaat tcgctgacat aacgctgtac tggcgcttcc
    12481 cagtggcgct ggttggacat gttgatggcg aattctttgg tcgattccgg cactttgatg
    12541 ttgtcatgcg tcagcacgaa gctgcccagt tcgcggtcca gggtgaagcc cttgacgcca
    12601 tcaccgaggg tcagcatcag catggtctgc gggccgtaga tcgcataacc ggcagcgacc
    12661 tgctcggtgc ccggctggag gaaagcctct tcaccgaggt cgccttcatt gccattgcga
    12721 tccgggcaac gcagcaccga gaagatggtg ccgaccgaaa cgttgacgtc gatgttgctg
    12781 gagccgtcca gcggatcgaa taccagcagg taggcgcctt tcgggtactt gccgggaatc
    12841 tggtaggcgt tgtccatttc ttccgacgcc atgccggcca ggtgaccgcc ccactcgttg
    12901 gcttccagaa ggatttcgtt ggaaatcacg tcgagcttct tctgcacctc gccctgcacg
    12961 ttctcggtgc ccatgctgcc cagcacgccg cccagtgcgc ccttggatac ctgatggctg
    13021 atttccttac aggcccgtgc gaccacttcg ataaggaaac gcagatcggc cggggtgttg
    13081 ttgctgcggg tctgctcgat caggtagcgg ctgagagtaa cgcgggacat ggatggctcc
    13141 ggagggaatg aaatcgcgcg cagtttaacc ctttagcggg gaaagcgcct tagcactgga
    13201 tggaaggggc tgcctggggc taggccagac tgcagaccct attacgcccc gcatgcttgg
    13261 cctgataaag cgcagcatca gccacctgca gcaattcgtc gagtaccagg ccctgttctg
    13321 gccacagcgc caggccggcg gacagggtta caaagcgcgc accgccacgg gtcggcagcg
    13381 ggtgcgcggc cagttcgcgg cagatggttt ccagacgcgc gcgagcctca ggggcatcgg
    13441 cgcgcggcag gatcaacagg aattcttcgc caccaatacg gaataccgca tccgagctgc
    13501 gcagattctc cagcagatgc tgagccacgc ccttgagcac atcgtcgcct tcgaggtggc
    13561 catgttcgtc gttgagctgt ttgaagtgat ccagatcgat cagtgccagg gagatcggcg
    13621 tgcgctcgcg ctgcgccacg gcaagttcac gggcaaagaa ttcgtccaga taacgccggt
    13681 tgtacagccc ggtcagcggg tcacacagtg cctgttcctg cagttgctcg tgcaggttgg
    13741 tgatggtacg tagccgttct tcgcttaggg ccagtgcttc ggccagtttt aactcgctga
    13801 gatgccgctg agtgacatca cgcaggtaga gcatctgccc gagtaccagg gtgccgcgat
    13861 gggtcacccg ctcgatggcg cggatacgca cttcgaaata tcgcgccgag ttggccaggg
    13921 tcagcagccg atcatgctcg ccctgcgtct gctggctcag caactgttgc aaatccgcac
    13981 cgaggatcgg ccaatcggcc agctgccggc cctgccagcc actgcgcagg cccgccaact
    14041 tcaaagcggc ggggttcgcc tcgatcaccc gcaactgcgc atcgaccacc agcaccggat
    14101 caagcaaggc atccagcagc agatggcggg ccaccggcag caggtcgaac aaccgcacac
    14161 caacgatcag ccaggcaaat gccaccaggg taaaggcgaa gctgaacggt gtcgggtcga
    14221 agccgaacag catccagcca aacactacat aactgatatt ggcgacccag ggcaccaccg
    14281 tgacgaagac aaacgccagg taatgccgcc gatgcacgcc atagctgacg agcgccgcac
    14341 gcaacaccac gcccatgcag aaagtcataa acaggtagac gtaagcggcg gcggcgtaga
    14401 acagcggccc atgctgatag cggatcggcg cgcccggctc atcactcagc ggcgctgtac
    14461 cggccccgta gaacaaacca tgccaggggt tgctcagggc catcagccag atcagcaacg
    14521 gcaccaaagc caggctcagg gtgctgcgca gcggcagcgg ctgacgcacg ctgttgacat
    14581 actgccagag gaaaatcgcc cagaaggtgg gcgttgtgag gatgcccggc caagcaatgg
    14641 tggcccagaa caccttgcat tgcgggcctt gcgcagccat ttccaggctc gcggcgatca
    14701 accaccagag actcgccagg tgcagcacga taaagctctc gcgcccgggg aaatagcgtt
    14761 ggcgtgtgac ccagcgcgcc agcagcagca cgcccacgca gaccacagaa gtcaggatca
    14821 ccggggcgct aagcgcccag gcggagtcac cgcaggcatt catcggtagg gtactgccag
    14881 ggaaaacggc tgtgtacgcc taccgatcga gtcgggcaaa cgcacgagaa ctagccctag
    14941 acggggcacg gtaaaaatac gcaagggcct tcctgacctc aaacgccaat caattgaagg
    15001 tgccaacaat actgcaaaat ccgttaggta gccgggcttt atttctgcta acagcctcta
    15061 ttcagcgctc ggccggcgca acagactgac cgccatcagc gccagcacgc ctaccccaag
    15121 cagcagtacc agccacaaac cccagcgttt ccagtcgctc tcgccaactg cctgctcttg
    15181 ctgcgccagc ccctcaatct gcaactgcgc ttcggctgta cccagttgct gcagacgctg
    15241 cggctgatag ccgggaatca aggtggtcaa cggcaaggcg gccgatttgg cacccgcacg
    15301 ccccaacgcc aaccgatagg gcggactgcc acgctggaga aacaccaact gggtagcgcg
    15361 caccgcgact ttgatctgcg gcggctcgac acccagaccg ccaccccgct catccacctg
    15421 cagacgcaac tgctgcaccg cccagcctgg caatggcagc tcatcctgac gaatttccac
    15481 gccattttcc ggcagccgat agagcaagcc ctgagccagt gattgccaag cacccttggc
    15541 ttcactgcga ccagtgacac gaatcggcgc caggctattg gcctgactga gttcgacgtg
    15601 cacacgctcc agcggcaagg ccaggggcat ttgccactga taatggccat ccggactgcg
    15661 cgtcgccgcc agaggctgcg accacaccag tggtgccggc acgctgtcac tgcgctgact
    15721 acgcaggcta gctgcggtca gctgtggtgc ttgttgcggg ttttgccaga gcaggcgcag
    15781 gtagcgcgtc ttgttacccg gcagttcgat ctggcgctgc tcgacacgct caccatcgaa
    15841 agataagcgc gccagctgac cactgcccca aggctgccag tcttgcaggt catcactggc
    15901 ttcgatgctg aagcgctgaa aaccctcctg accgccgctc cagtccagct gcaggcgcac
    15961 cagcggctcg tccaatgcgc tggcatccag cagccagcca cgcagctgcg ccggcttggc
    16021 ggcggcatcc gattcttcga gcagctcgat cagcgtgccg ctggtgctgc gttgtacccg
    16081 cagactgggc gtagcattgc tgtcgccttg ctcggcatac agcggaaacc acttcaccgc
    16141 ctgttcctgc tggctatcgc gcgactcgcc ctgccccgct accagcgcgt aggccaaggc
    16201 ctggccttcc gcgttaaaca cccgcaggtc acgcaaatcg ccatagcgcg cggcgaaatg
    16261 cagcgccatc ggcacatcca ggctatacca ggggccttcg ccactggggc ttagcggcag
    16321 ctgcatggca tagtcgccag gctgctcctg cgccagggct ggcgcactgc acaacaaacc
    16381 aagcaacgca acgccgccca cgcggtgaat taaacgactg atcatgcttg ctcctgcgcc
    16441 tgctgcgcat ccgccttgcg tgccggcaag ggggcgaaat agcccaccac cagcagcaag
    16501 acacccaccc caataaacga cacgatgcgc tccaggccac cgcgattacc caattcgacg
    16561 aagaacagct tggccacgac cagggcgatc agcgccgcgc cgatcaacca cagctcgcgc
    16621 cggccactcc ggttgccgag gaccatcagc ggcagggcga tcagcgtcca gacgatcgac
    16681 agcccagcct gcaccagcat cgaatcgagc agcgcatcca gctgataagg cacgccgccc
    16741 cagtggtgag ccgtgcgcat caccatagcc gtcagcaggg caaacagcga agcccccgtc
    16801 acagcttgag tcagccactg gctgcgtacg ctatccacgc ccaactcggc cagccccatg
    16861 cgcgcccagg cgaacaccgc gccaagggca aacagcaggc ccagctccag cggattgagc
    16921 agcggcagat aggctagcgg ttcagcgtcc ccggcactga ccacgttggc cagccagaac
    16981 cagccaagca tcagcagcgc caacggcgct gctgcgagca gacggtattc acgcggatag
    17041 gccgccagcg gccatggcca gcgacgtgac ggcgtcatct gccagggggc catcagccat
    17101 aaataggcac ttggcaacag cgcccaaccc aaccagcgcc aggcgttgta atgctctgat
    17161 agcgccagta gcaggtagcg caactccagc gccagcacgc ccaacagcaa ccagcagccg
    17221 agcacatggg ccgcgctcag tgcacccgct ggcaactgtg cagccagacg ccgtagcgcc
    17281 cagaagtgca cagcgaacag cagcgtccag gccagccagc cgaactgcgc cgcggggtga
    17341 tagtgcagat gccaggcatg cagcagcacc acaccggcca gcggcgtcag caggcagcac
    17401 agcagcgcca gttcggccca gcgcgtgcgc ggcgcaagga atgccgccag ggcgacgctg
    17461 gccgccagca ccgcgagcaa ggcgctgctc tgccattcaa cggcgacaaa gcgcagcact
    17521 tcactgctta gcgccagcgc ccaccaggca gcgccccaca ccagcagcaa ttgcgccaga
    17581 ccaagcaaac tgaaattgcc aagggcgctg ggcgcctcgc gccgcgccag ctgttgcaag
    17641 cgccaggcgc cgaccaaggc ggcgacgcca agcaccagcg gcgtccagaa atccacatgg
    17701 gccagcggcc gaagcccctc ggccgacagc tggccgaata gcagcgggct gactgcgaga
    17761 aacaccagcc ccgccaacac ctgcaacaac agaccgaaga caaaactggc gcgttgctgc
    17821 aggtgaaggc tgagccagat gatcaacaga ccgctgccgc cccataccgc gctggccgta
    17881 gcccagggca ggacgaacag caccgccaag ttgaggaaca ccagtcccac cagtagcacc
    17941 accgacaggc cgcgcagcag gcggcgatcc gcgccgacct gcggatcacg ggccgccagc
    18001 agcatgccgg cgatcaagcc aaagccaatc agcgatgagg tcagcaagcc ctgccaaccg
    18061 gcagagaagc cgcccatacc agtggccgcg ttgccggcca tgcccagcat aaacaccagg
    18121 ccaccgagca actgcacggc aaaggcgctg aacaggaaac tgcgcgcctg caggcgcaag
    18181 cccagccaca aggtcagcag gccggccagc gcccaggtga tcgccgtgcc ttgggcggca
    18241 aacaacagcg gcgcaatcag atagaggaag gccaatccgg cgcaggccag caccggcagc
    18301 ccgcgcactt cccaactgct ggcgtgttgc gtgttctgcc gcaactgcca gtagctgaac
    18361 agcaaagcca ccccaagcat cagcgcgccc agcggcgcgc catccagcag ggtcagctcg
    18421 cccgcacgca ggccgatggc aaacgccagc gccgcgccaa actgcaacag cagggcaaac
    18481 gcccgggcca ggctgcgttg ctgacgcagg cccagccaga agatacccgc accttccacc
    18541 gcccaggcgg ccgaggtcca gcgcgcatcc aggcccagcg gaatcgccag actgccgaac
    18601 accacaccga gcgccaggca ggtttccacc agcagcacag cacgatcacc cgtccggccg
    18661 accagcaacc gcgccaacac gatatagaac agccccagcg ccagggcact gaacgcagcg
    18721 gcaaattcga tgtgctgcac cagggcgaac tgcagaccaa aacccaccag tggcgggccg
    18781 aacagcacgc tggcatcgat atagtcgccc ttacgcgccg cccaacgcag cagtgcatcg
    18841 cgatcctcgg gcgcatccag tgcttcgcgc agcttgcgcc gggcaaacag cagaccgatg
    18901 gccacataca tcaggaagaa cagaatcaga aacggttcgg tgctccacag cagttccggc
    18961 gtgtaggagc ggatgcccca ggcgaaaccg atgccgaagg tgccgacaaa accgatcagg
    19021 ttgagcaggc gccaggcctt gaaccaggcg atggcaaaga tcccggcatt gagcagggcg
    19081 aagtaactga acagcgccac atgattgccg ctgccggtag acgtcaggat cggcgcggcg
    19141 aaaccgccca aagctgcgac ggccgccagg cccaaggcat tctgcttgac cgccagaatc
    19201 gccgagaaca ccgtcaccag caccagcaga cccagcgcca cgccagggtc cagcaacgga
    19261 tgcaggcgca tggcggcaaa caccgtcagg tacaacacgg caatcccggt gccctgcagc
    19321 atcagcgcat agttggggtt acgaaggcgc agccaccagc ccagagccaa cagcgccacg
    19381 gcactggcgg cgacgctggc ataacgcaac tccaccggca ccaccacgcc ttcggtggcg
    19441 taacgcagca ggaaggccag accgagaaac aacagcacca caccgacgcg cagcaccgtg
    19501 ttgccgccca gcaaccagtc cttcgctgcc gtaaagcccc gctcaagcaa ggttggctga
    19561 cgcggctcag caggtttcca gggcgtagcg ggcggcggtt tgattggcgc agcggccggt
    19621 tcggccgtag cctgcatagg cgcgacaggc tcaggagcag gttccggcag gacaaaatcc
    19681 agggtccagt ccagttcggg ctgggccgct tgggcactga cgatgggctc aggtacaggc
    19741 tcgacaacag agttgaccgg ctgcggcgtt ggtggcgcct caggggctgg catggcgctt
    19801 tgctctagct taagcaaacg ctcatacagc gccgctgtgc cctgctcgaa gcgttgagaa
    19861 aaggccttga gctgcgccgt tagcgcggca ttttcgcgct ccagcccatg cagcttgagc
    19921 gcctgaccaa ggcccagacc aatcagagca cccagcaacg aggcagtcag cgtttcatcc
    19981 gccaacgccc cgagcaccaa gcccaccagc atgaaaatcc attgcatgca gcgccttcct
    20041 attcagctaa acccgaacat cctgttgccc ggcacccggc gcaaactgcg cagcagtata
    20101 ggggcccgac cggggcatac ccagagagac gaatgccgca cgcatcacag cacagtaaga
    20161 aattgccccg gccgcaaccg cacaaaatat gaccagctgg cagatagtca ttcacaaacg
    20221 cctcaaggcc gctatcatcg cgcgccgcat aactgatgca tgcctgctcc gatgaaaaac
    20281 aacctgattg ccgccgccga actcgaccgc ctggaaacct gggccaaata caccgccgat
    20341 atgtgccaca gttgcatgtc cagctgctgc acgctgccgg tcgaggcacg tctgaacgac
    20401 ctgattcgta tcggcgtggt cgatgagttc gaacgcagcg agccgctgaa gaacatcgcc
    20461 aaacgcttgc agaaagacgg catcgtcgaa cgcttccatc aaaagtcggg catcttcacc
    20521 ctgacccgca tgagcaataa cgactgctac tacctcgacc gcaagacccg cctgtgcacg
    20581 atctacgaca agcgccccga tacgtgccgt aatcacccga agatcggtcc gcgcccaggc
    20641 tattgcgcgt ataagcccaa agcctgagct caccccgcgc gccactggtt tatcctagac
    20701 gctccgattg cgacctaggc caacctgcat gtccccatgg cgctccctca gtctgtggat
    20761 ggatcagctg gacgaaccac tgaccgcccg ccccgcgctg accgaagacc tgcacgccga
    20821 tgtggccatc atcggcgcgg gctacaccgg cctgtggacc gcctactacc tcaagcgcca
    20881 ggcgccacag ctgcgcatcg tcatccttga agacgagatt gccggtttcg gcgcctccgg
    20941 gcgcaacggc ggctggttga tgggcaacct gctcggccaa gaccgcctgc tcgccgacca
    21001 gcccagtgcc gagcgccaga gtgcctatgc cctgctgcac ggcattcccg atgaagtcgc
    21061 cagcgtgctg cagcgcgaac agatcgcctg cgattaccgc aaagccggcg tgctgtattg
    21121 cgccgcgcgc taccccgagc agctgaccag cctgcgccag cagctagccg aattgcgtgc
    21181 actgggcctg gatgaaaacg actaccgctg gctgacgccc ttcgacctcg gcaagcaact
    21241 gcagctggcc aacgcctacg gcgccatcta caccccgcac tgcgcgacca ttcaaccagc
    21301 caagctggtg cgcggcctgg cgcgctgcgt agcagccatg ggcgttgagc tatatgagca
    21361 gagccgagtg atcgactggc aaaccggcct ggtgcgcacc gctagcggca gcgtgcgcgc
    21421 tgactggatc gtcccagccg tggaaggtta cgccgcctgc ctgccgccgc tgggcaaaca
    21481 tcaaataccg gtgcagagcc tgctggtggc caccgagccg ctgcccaaag aagtctgggc
    21541 cgatattggt ctcgagcgcg gccaggcctt cagcgagaac agccgtcagg tcacctacgg
    21601 ccaacgcagc gccgacgacc gtctggtatt cggcgcacgc ggcggctacc gctttggcgg
    21661 ccagttgcgc accgacttca acctcagcga gaacgagcgc gacctgcgcc gcgacctgtt
    21721 tggcgaactc ttcccgcaac tcaagcaggt acgcatcacc catgcctggg gcggcaacct
    21781 gggcatggcg cggcgctttc gcccgcatat gctggtggac cgcaaacaag gcatcgccct
    21841 gtccggtggt tacggcggcg agggcgtggg cgcgagcaac ctcggcgggc gcaccctggc
    21901 cgacctgatt ctcgaccagc agagcgagct gacccgccag ccttgggtgc taggcgacca
    21961 gccgatcaac cgcctgccgc gctgggagcc ggagccgctg cgctggctcg gctacaacgc
    22021 gatcatccag agtttcgcgc ttgaagaccg cgtgctggcc aacccagaca gcgcgccctg
    22081 gcggcgcaag ctggccagca gcctggccgg caccctggaa aaacttatgc ggtaacggtg
    22141 cgttttataa gcagcgaatc gagcatgaag tacaacgcat cgcggatagg tgacatgccc
    22201 gcacattgcc tgtcgtagcc agccaactgc tgagcgaaca gcgccttgaa ttcttcgtca
    22261 cgcataaaag ctctccgctg catcctggat gaatgatgga ggctggcttc cagtatccaa
    22321 gagccgtcaa gccacacttc aacagcgggg cttagagaat ccttaagcaa ctttgctcag
    22381 attgtggggg actaacagac ataactctcc agtgaagcgg ctatgcagat tcccaacgtc
    22441 gaacagaaag ctttcatact cctcctcgtc cttgtgacag tagccttctt ctggatactg
    22501 ctgccgtttt acggtgcggt cttctgggcc atggtgctgg cggtggtatt tacccctttg
    22561 caacggcgca tggcgcggcg cattggcggc cggggcaatc tttcagccct gctgacgctg
    22621 gtcgtatgtc tgctggtcgc catcctcccg gtgatattta tcacctcagc ggtcgttgcc
    22681 gaaggtgcca gcctctatca gaagctggag tcgggcgaac tggatattgg gtcctacgtc
    22741 accagcacca tccgggcgct gccgccgacg ctgcaagaac agctcgaccg catcggcgtt
    22801 tccgaccttg atggcctgcg cgaacagatt tcaaccgtgg ccatggccgg cagtcagttt
    22861 ctcgccacca aggtgttcgc catcggtcag ggcaccttcg cattcttcgt cagcttcttc
    22921 gtcatgctct atctgctgtt cttcctgctg cgtgatggcc tggaattggc gcgcacaatt
    22981 cgcgaaacca ttccgctggg cgaatccacc aaacgccgcc tgcagatcaa attcacccga
    23041 gtggttcgcg ccacggtaaa gggcaatgtc gtagtcgccg cagttcaggg tgcactgggc
    23101 ggccttatat tctgggcgct tggcatctac agcccgctgt tgtggggcgt gctgatggca
    23161 tttctatcgc tgctgcccgc cgtcggggcc ggcattatct ggttaccagt ggcggtgtac
    23221 ctgatactca gcggcagcct ctggcagggg gtgatcctca ccgtcttcgg cgtgctggtg
    23281 atcggtctgg tggacaacat cctgcgcccc atcctggtgg gcaaggacac ccgcatgccc
    23341 gactacctga tcctgatttc cactcttggc ggcctggccc tgctgggcct caacggcttc
    23401 gtgatcgggc cgctggtcgc ggcactgttc ctctctagct ggaacctgtt cgagggagga
    23461 aaaaggacgg tcaaattgcc ggactagcca agcctcaaac gcaattaacg aattaaaagc
    23521 ggcctgcaac accgccctgg gacagccaac aaaaagcccc cgcaggctcg cgccggcggg
    23581 ggctttttag ttagctagaa ctggtatcag ttcttggctt tcttggcagc gcgggtgcgc
    23641 tcgccttcgt cgaggatctt cttacgcaga cggatcgatt tgggcgtaac ttcgcacagc
    23701 tcgtcgtcct ggatgaattc cagagcctgt tccagggtga atttcaccgg cggaaccagg
    23761 gcgatggttt cgtccttgcc ggaagcgcgc atgttgtcga gcttcttgcc tttggttggg
    23821 ttaacgccca tgtcgttgtc acggctattc agaccaacga tctgaccgtt gtagatctct
    23881 tgaccgtgct caacgaacag cttgccgcgc gcctgcaggg tttccaggga gtaggtcagc
    23941 gccttgccgg tttctaccga taccagcaca ccgttctgac ggccggacat gtcgccggac
    24001 ttcatggtgt cgtagcgatc gaagatcgag gtcaggatgc cagcaccgtt agtcagggtc
    24061 aggaactggt tacggaaacc gatcagaccg cgagcaggga tgttgtattc cagacgaaca
    24121 cggcctttgc catccggcac catgttggtc aggtcgccct tacgcaggcc catctcttcc
    24181 atgaccttgc cctgggactc ttccggggtg tcgatggtga cgttttcgaa cggttcctgc
    24241 ttcacgccgt cgaccgtacg gatgatcact tccggacgac ccacacccat ttcgaagcct
    24301 tcgcgacgca tggtttcgat cagtaccgag aggtgcagct caccacggcc agagaccttg
    24361 aacttgtcag cgctgtcgcc ttcttcaaca cgcagggcaa cgttgtacag cagctctttg
    24421 tccagacgct ctttgatgtt gcggctggta acgaacttgc cttctttacc gcagaacggc
    24481 gaatcgttga cctggaaggt catggatacg gtcggctcgt cgacggtcag cggggtcatc
//
LOCUS       DASRUM010000015        12410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1345878, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000015 DASRUM010000000
VERSION     DASRUM010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 12410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12410
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345878"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..892
                     /locus_tag="VFF22_01075"
     CDS             2..892
                     /locus_tag="VFF22_01075"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HZX15447.1"
                     /translation="MAKIGFIGTGIMGKPMAQNLQKAGHTLFFSEHFDKAPADLLGDN
                     GIGLANPREVAQEAEFIIIMVPDTPQVEDVLFRADGVAAGVGAGKVVIDMSSISPTAT
                     KVFAEKIKATGAAYLDAPVSGGEVGAKAATLSIMIGGCPNAFERALPLFQAMGKNITR
                     VGGNGDGQTAKVANQIIVALNIQAVAEALLFAAKNGADPAKVREALMGGFAGSKILEV
                     HGERMIKGTFDPGFRISLHQKDLNLALAGARELGLNLPNTANAQQVFSTCAAIGGSNW
                     DHSALIKGLEHMANFNIRGE"
     gene            1060..2346
                     /locus_tag="VFF22_01080"
     CDS             1060..2346
                     /locus_tag="VFF22_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HZX15448.1"
                     /translation="MSIDPSFNPTSLLRELFASAIDAAHPRQVLADYLPEDRSGRVIV
                     IGAGKAAAAMAEVIEQEWQGEVSGLVVTRYEHGANCKKIEVVEAAHPVPDDAGERVAR
                     RVLELVSNLSESDRVIFLLSGGGSSLLALPAEGISLKDKQAVNKALLKSGASIGEMNC
                     VRKHLSAIKGGRLAKASWPASVYTYAISDVPGDEATVIASGPTVADPTTSAEALAILA
                     RYSIEIPANVHAWLQNPASETVKPGDPCLARSHFQLIARPQQSLDAAAAKARAAGLPT
                     LILGDLEGESREVAKVHAGIAKQVRLHGQPIKAPCVILSGGETTVTVRGNGRGGRNAE
                     FLLSLTDSLKGLPGVYALAGDTDGIDGSEDNAGAIMTPDSYARAAALGLSSSDELDNN
                     NGYGYFAALDQLIVTGPTRTNVNDFRAILILENPAK"
     gene            2343..3758
                     /gene="pyk"
                     /locus_tag="VFF22_01085"
     CDS             2343..3758
                     /gene="pyk"
                     /locus_tag="VFF22_01085"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZX15449.1"
                     /translation="MNADKKVKILATLGPAIKTIDDIRQLVEAGVNLFRLNFSHGEHA
                     DHAQRFDWVREVEQQLNTPIGILMDLQGPKLRVGRFAEGKVNLQRGQTLRLDLDSTPG
                     DVNRVNLPHPEIIEALQPGMSLLLDDGRLRLQVTAKHSDAVITEVIAGGELSDRKGVN
                     VPEAVLQLSPLTEKDRRDLSFGLELGVDWVALSFVQRPEDIIEARQLIGDRAFLMAKI
                     EKPSAVTHLEEITKLCDAIMVARGDLGVEVPAENVPRIQRDIIRTCRQLGKPVVVATQ
                     MLESMRFSPAPTRAEVTDVANAVAEGADAVMLSAETASGDYPLEAVQMMSKIIRQVEN
                     GPDFQAQLDVSRPQAEATASDAISCAIRRISTILPVAVLVNYTESGNSSLRASRERPK
                     APILSLTPSLDTARRLSVAWGVYSVVNERLHKVEEVTRTALETARSAGLAKTGDTVVI
                     TAGEPFGQPGSTNSLRIEILH"
     gene            3899..4852
                     /locus_tag="VFF22_01090"
     CDS             3899..4852
                     /locus_tag="VFF22_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HZX15450.1"
                     /translation="MSPLPLAPHALQGSRALLNGFSQIFLQAHPGCGLLVLFAIAIGA
                     PQLLGGALLGCLSSTLTAQRRGYAKADIDLGLYGYNGVLLGMLISLQFAWSALLPLLI
                     IVSAGASSLLLAAWMQRMRERQWLPAFTFPFVGLSWLLLWLAPVLQLELQPAAASQAQ
                     PLDWWASLIAMARGLGQVIFLDQPLAGVCLLIGLLLADRRAACWALLGSTGGLALALY
                     QGLPQHSALTGLYGYNAALAAIALSQVHRRPWLPALGIALALVLQPGFSALGLPALTM
                     PFILTCWLVSATLRLLHKAAADCTPLPLKKGKDLHRHDKTP"
     gene            4912..5736
                     /locus_tag="VFF22_01095"
     CDS             4912..5736
                     /locus_tag="VFF22_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HZX15451.1"
                     /translation="MDTRQKLYSIIFHTDTPAGQRFDRYLLLAIMASLVVVMLDSIDS
                     VHSQYAGLLAGLEWGFTALFAIEYVVRLYCSPKPLRYAFSFFGLIDLLAILPGILALF
                     YADAQYLLIIRVIRMLRVFRVLKLSPYLSQANFLLTALRGSKQKIIVFFACIATMVTV
                     FGTLMYVIEGPAHGFTSIPKGIYWAVVTLTTVGFGDIVPKTPLGQALSTLVMITGYSI
                     IAVPTGIFTAELANAMRGDGQMMHDCPTCAKSTHEHSAAFCSRCGNPLFPRQTDKP"
     gene            6032..6448
                     /locus_tag="VFF22_01100"
     CDS             6032..6448
                     /locus_tag="VFF22_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HZX15452.1"
                     /translation="MPLLKLLHFIALIGWCGALLYLPALIAAGTRSNEALFYRDHAHL
                     TRMVFTLVATPAALLAIGSGTLLFVRESVFDPWLILKLSAVAGMVLCHALCGVLILRI
                     ERTPARAIGHHCLGIGALLVVLITATLWLVLAKPAF"
     gene            complement(6505..6963)
                     /locus_tag="VFF22_01105"
     CDS             complement(6505..6963)
                     /locus_tag="VFF22_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HZX15453.1"
                     /translation="MTDKNDSIFSRAAIAGHPLHPMMIHFPVAALLGLVASDLAYLWS
                     DDPFWWRASLWLAGIGSLGGWLASLAGLIDLLTVERIRLKITAWCHAIIAVMMLSLAT
                     LNWLLRYQGLDGMQLWCLYLSLLTAGLIALAAYLGGRLVYEQAVGVEVNL"
     gene            7099..7989
                     /gene="coxB"
                     /locus_tag="VFF22_01110"
     CDS             7099..7989
                     /gene="coxB"
                     /locus_tag="VFF22_01110"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HZX15454.1"
                     /translation="MAREVALLWWLMCGFAGLVLLVVSALWIYAMRRTPQEITPEQAK
                     RINLRWLVGGGLLLPGLSITVLLFFGGSIGERMLPLPLLGEQPLRIEITGHQWWWEVR
                     YPDSGVVTANQLHIPAGRPVDLQVSSADVIHSFWVPRLGGKIDMIPGRHNRIRLQADE
                     PGIYRGQCSEFCGTQHSHMILHVQAHAEDGFNAWLSGRQNAQISPPAGAAGEIYATRC
                     GICHRVAGVSAGQRAPDLSDLGSRPTLGSGVLTNEPGAILTWLREHQRLKPGNAMPLH
                     DDIDPAHLHTIATWLEGLEP"
     gene            7986..10514
                     /gene="ctaD"
                     /locus_tag="VFF22_01115"
     CDS             7986..10514
                     /gene="ctaD"
                     /locus_tag="VFF22_01115"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HZX15455.1"
                     /translation="MSSRNHAPATDPDQLHDQFNAVWGNPKGWRALTIVNHTTIGLRF
                     MLTGAVFFLFGGLLAMLIRTQLALPNHEFMEPDVYNQVFTMHGTVMMFLFAVPMMEGM
                     AVYLIPKMLGARDLVFPRLSALGYYCYLFGGLILITSLFLDLAPKAGWFMYTPLSSAA
                     HTPGPNSDFWLIGITFVEISAVSAGVELVVSILRTRASGMALHKMPIYAWYILVMAMM
                     IVVGFPPLILGSILLELERAAGMPFFEVARGGDPILWQHLFWLFGHPEVYIIFLPAAG
                     IVSTLLPVFCQRPLVGYRWVVLGVLTTGFISFGLWVHHMFTVGIPQLAQLFFSAASML
                     VAIPTAVQVFAWLATLWQGKPVYHVPMLWLVGFLIVFVCGGLTGVMLALVPFDWQVHD
                     THFVVAHMHYVLVGGMFFPMMAGIYYWLPHFSGRMPSVRLGKWGFWLVFIGFNVTFLI
                     MHWTGLIGMPRRIYTYQTGLGWDIPNLISSVGSFIMAIGVATILLDIILHFRFGQPAK
                     VNPWRADTLEWATSLPPSAYNFVSLPAIHSRHPLWDNPGLPDSIARGEHELAVVDHGR
                     RETWGTDPLTGKVREIIHLPGNSWIPLIAALFIAAMCISLLVKVYWLAAVFFAAAVLT
                     LLRWSWENGTHPLAAPDARVQPGEPPLHSRTLDGPGLWGMGVTLLANGALYLALLFGW
                     FYLWTVAPNWQMPQHIPFNQWLLLASAVLLSLATWWQARAIKRLRHGNAQGLRGHLAL
                     VACLGLGQSGLLLWTLLGGELAPRATAHDAVIVVVLIYSLLHCALAATLSALQAIRVH
                     CGYVGAHAPYEPLVVGHLWYYNLGVLWSSYFALVLFPSTWGGSG"
     gene            10511..10864
                     /locus_tag="VFF22_01120"
     CDS             10511..10864
                     /locus_tag="VFF22_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15456.1"
                     /translation="MKGLRSPYHPGHIPLGLVFWSLWFVALYGGLSVACALAPPEPEQ
                     GPWNWLNALLGVSALLTAAALAALAIWCWRAGKPQPSPGHQRFVALLAAGTYGVATVA
                     TLVIALPILSLPPCM"
     gene            10855..11676
                     /locus_tag="VFF22_01125"
     CDS             10855..11676
                     /locus_tag="VFF22_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003294822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HZX15457.1"
                     /translation="MHVITALCLLLVMPTAQAHGLLDHQLNLRVPLLLSALMLATAWV
                     LYGLGARRVRPHGLEALWLHLAMLIAVLAVFGPLDDWAETSTSWHMLQHMLFMLVIAP
                     LWALARPLPQWRAVCGPLLQPLWRGVLRAGRYPVPLAMLHGALIWIWHTPSLYILALE
                     NLWSHMLEHASFLFSAWLFWWACLRAPPRQVPQALLAILLTLMHTGLLGALLTFGTSS
                     FYGAERDLADQQLAGLLMWVPGGLVYLAAAGWITWRWLARSWRHQQSARASKGSA"
     gene            complement(11715..>12410)
                     /gene="argR"
                     /locus_tag="VFF22_01130"
     CDS             complement(11715..>12410)
                     /gene="argR"
                     /locus_tag="VFF22_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015885478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HZX15458.1"
                     /translation="GCVIGAIAAGVYPLAQLGLLDGYRASVHWRWQDDFTERFPKVIA
                     TSHLFDWDRDRLTACGGLAVLDLLLALLARDHGAELAGAVSEELVVERIREGGERQRI
                     PLQNRLGSSHPKLTQAVLLMEANIEEPLTTDEIAQHVCVSRRQLERIFKQYLNRVPSQ
                     YYLELRLNKARQLLMQTSKSIIQIGLSCGFSSGPHFSSAYRNFFGATPREDRNQRRSN
                     SPFELTSTPIERG"
BASE COUNT         2153 a         4106 c         3764 g         2387 t
ORIGIN      
        1 catggcaaaa atcggattta tcggcaccgg catcatgggc aagcctatgg cgcagaacct
       61 gcaaaaagcc gggcacaccc tgttcttctc cgagcacttc gacaaggccc cggccgacct
      121 gctgggcgac aacggcatcg gcctggccaa cccgcgcgaa gtggcgcagg aagccgaatt
      181 catcatcatc atggtgccag ataccccgca ggttgaagac gtactgttcc gcgccgacgg
      241 cgttgccgca ggcgtgggcg ctggcaaagt ggtgatcgac atgagctcga tctccccgac
      301 cgccaccaag gtcttcgctg agaagatcaa agcaactggc gctgcctacc tcgacgcgcc
      361 ggtatccggc ggtgaagtcg gtgccaaggc ggccaccctc tcgatcatga tcggcggctg
      421 cccgaatgcc ttcgagcgcg ccctgccgct gttccaggcc atgggcaaga acatcacccg
      481 cgttggcggc aacggtgacg gtcagactgc caaggtcgcc aaccagatca ttgttgcttt
      541 gaacatccag gccgtggctg aagccctgct gttcgctgcc aagaacggtg ccgatccagc
      601 caaggtgcgt gaagcactga tgggcggttt cgccggttcg aagatccttg aagtgcacgg
      661 tgagcgcatg atcaagggca ccttcgaccc aggcttccgt atcagcctgc accagaagga
      721 cctcaacctg gcactggccg gtgcgcgcga gctgggcctt aacctgccca acaccgccaa
      781 cgcccagcaa gtgttcagca cctgtgcggc catcggtggc agcaactggg atcactcggc
      841 gttgatcaaa ggcctggaac acatggccaa cttcaacatc cgtggtgagt aagtaacagg
      901 tttcagggcg gggtcagccg cgctgaaaca accctttcga tctgcccggc aggcggtgtc
      961 acggcctgcc ggccgggtgg atcgattcta atgcagagcc tctgccaaac ggacgctgcg
     1021 cactagaatc gaagttgatc cagaccaaga gagatcacca tgtccatcga tccgtctttc
     1081 aatccaacgt ctctgctgcg cgagctgttc gccagcgcca tcgacgccgc ccacccgcgc
     1141 caggttctgg ctgactacct gccggaagat cgcagcggcc gcgtcatcgt gatcggcgcc
     1201 ggtaaagccg ccgcggccat ggccgaagtg atcgagcagg aatggcaagg cgaggtctcc
     1261 ggcctggtgg tcacccgtta cgagcacggc gccaactgca aaaaaatcga agtggtcgaa
     1321 gcggcgcacc cggtgccgga cgacgccggt gagcgcgtcg cgcgccgcgt gttggagctg
     1381 gtcagcaacc tgagcgagtc tgaccgggtg atctttctgc tgtccggcgg cggctcttcc
     1441 ctgctcgcac tgcccgctga aggcatcagc ctgaaagaca aacaagcggt caacaaggcg
     1501 ctgctgaaat ccggcgcctc gattggtgag atgaactgcg tgcgcaagca cctttctgcc
     1561 atcaagggcg gccgcctggc caaggccagc tggccggcca gcgtttacac ctacgcgatt
     1621 tccgatgtac cgggcgatga agccacggtg attgcctccg gcccgaccgt ggccgatccg
     1681 actacctccg ctgaagccct ggcgatcctc gcccgctata gcatcgagat tccagccaac
     1741 gtgcacgcct ggttgcaaaa cccagcctcg gaaaccgtca aaccgggcga cccgtgcctg
     1801 gcgcgcagcc acttccagct aatcgcccgg ccgcagcaat ccctcgatgc cgctgctgcc
     1861 aaggcgcgcg ccgccggctt gccgacgctg atcctcggtg atctggaagg cgaatcgcgg
     1921 gaagtcgcca aggtgcatgc cggcatcgcc aaacaggtgc gcctgcatgg ccaaccgatc
     1981 aaagccccct gcgtgattct ctccggcggt gaaaccacgg tgaccgtgcg cggtaatggc
     2041 cgtggcgggc gcaacgccga attcctcctc agcctcaccg acagcctcaa gggcctgcct
     2101 ggggtttacg ccctggctgg cgacaccgac ggcatcgacg gctcggaaga caacgctggg
     2161 gccatcatga cccccgacag ctacgcccgc gctgcggcgc tgggactaag cagcagtgat
     2221 gaactggata acaacaacgg ctacggctac ttcgccgccc tcgatcaact gatcgtcact
     2281 gggccgacgc gtaccaacgt caacgatttt cgcgccattc tgatccttga gaaccctgcc
     2341 aaatgaacgc cgacaagaaa gtaaaaatcc tcgccaccct tggcccagcg atcaaaacca
     2401 tcgacgacat ccgccagttg gtggaggctg gggtcaacct gttccgcctc aacttcagcc
     2461 acggcgaaca cgccgatcac gcccagcgct tcgactgggt gcgtgaagtc gagcagcagc
     2521 taaacacccc aatcggcatc ctgatggacc tgcaagggcc gaagctgcgc gtcggtcgtt
     2581 tcgccgaggg caaggtcaat ctgcaacgcg gccagaccct gcgcctggac ctcgacagca
     2641 cccctggcga cgttaatcgg gtcaacctgc cccacccgga aatcatcgaa gcgctgcagc
     2701 cgggcatgag cctgctgctc gacgatggcc gcctgcgcct gcaggtcacc gccaagcaca
     2761 gcgatgcggt gatcaccgaa gtgatcgccg gtggtgagct gtccgaccgc aagggcgtca
     2821 acgtgccgga agcagtattg caacttagcc cgctgaccga gaaggaccgc cgcgacctga
     2881 gcttcggcct ggaactgggt gtggattggg tggcactgtc attcgtgcag cgcccggaag
     2941 acattatcga agcccgccag ctgatcggtg accgcgcctt tctgatggcc aagatcgaga
     3001 agccgtcggc cgtgactcac ctggaagaga tcaccaagct gtgcgacgcg atcatggtcg
     3061 cccgtggcga tctgggcgtg gaagtgccgg ccgagaacgt gccgcgcatc cagcgcgaca
     3121 tcattcgcac ctgccgccag ctcggtaaac cggtggtggt ggccacacag atgctcgaat
     3181 ccatgcgctt ctccccggcg ccgacccgcg ccgaggtcac cgacgtagcc aacgccgtgg
     3241 ccgaaggcgc cgatgcggtg atgctgtcgg cggaaaccgc ctccggcgac tacccgctgg
     3301 aagctgtgca gatgatgagc aagatcattc gccaggtgga aaacggcccg gacttccagg
     3361 cccagctcga tgtcagccgc ccgcaagccg aagccactgc ctcggatgcg atcagctgcg
     3421 cgatccgccg catcagcacc atcctgccgg tggccgtgct ggtcaactac accgagtcgg
     3481 gcaactccag cctgcgcgcc tcgcgcgagc ggccgaaagc accgatcttg agcctgaccc
     3541 cgagcctgga taccgcgcgg cgtctgagcg tggcctgggg cgtgtactcg gtggtcaacg
     3601 aacgcctgca caaagttgag gaagtcaccc gcaccgccct ggaaaccgcc cgttcggctg
     3661 ggctggcgaa aaccggcgac actgtggtga ttactgccgg cgagccgttc ggccaaccag
     3721 gcagcaccaa cagcctgcgt atcgaaatcc tccactgatc cagctctgcg gcaggtcgcc
     3781 agccccgccg cagaacgcaa ctcccacgct ggctttgcca gttttgtagg cacccactgt
     3841 tcccgcggtg ggtgcctctt tttatggccc tggtgatcgt ctactcaggt tcgtctgtat
     3901 gtcgccatta ccgctcgccc ctcatgcttt gcaaggctct cgcgccctgc ttaacggttt
     3961 tagccagatc tttctacaag cccatcccgg ctgcggcttg ctggtgctct ttgcgattgc
     4021 catcggtgcg ccgcagctgc tcggcggcgc cctgctcggt tgccttagca gcacactaac
     4081 cgcgcagcgc cgcggttatg ccaaagccga tatcgatctc ggtttgtatg gctacaacgg
     4141 ggttctgctg ggcatgctga tcagcctgca atttgcctgg tcggcgttgc tgccgctgct
     4201 gatcatcgtc agcgccggag cttccagcct gctgctggcc gcctggatgc agcgcatgcg
     4261 cgagcgtcag tggctacccg ccttcacctt cccctttgtc ggtttgagct ggctgctgtt
     4321 gtggctggct ccggtcctgc agctggagtt gcagccggcc gcagcaagcc aggcgcaacc
     4381 actcgactgg tgggccagtc tgatcgccat ggcacgcggg ttgggtcagg tgatttttct
     4441 cgatcagccg ttggccggcg tttgcctgct aatcggcctg ctgttggcgg atcggcgcgc
     4501 cgcctgctgg gcgctactgg gctccactgg cggcttggct ctggcgctgt atcagggctt
     4561 gccacagcac agcgccctca ccggcctgta tggctataac gcagccctgg cggctatcgc
     4621 gctcagccag gtgcatcgcc gcccctggct accggccctc ggcatcgccc tggcgctggt
     4681 gctgcaaccc ggtttcagcg ctctgggtct gcctgccctg accatgccgt ttatcctcac
     4741 ctgctggctg gtcagtgcca ccctgcgtct actgcacaaa gctgcggccg attgcacacc
     4801 gctgccgctc aaaaagggca aagacttgca tcgtcacgat aaaactccct aggctgcctg
     4861 ttcgcgcgat cagcgatcag cgaacagcgc atacggagca gccagaccac catggacacg
     4921 cggcaaaagc tgtacagcat catctttcac accgatacgc cggccggaca gcgcttcgac
     4981 cgctacctgc tgctggccat catggccagt ctggtggtgg tgatgctcga cagcatcgac
     5041 agtgtgcaca gccagtacgc gggattgctg gcggggcttg aatggggctt taccgcgctg
     5101 ttcgccatcg agtacgtggt gcgcctgtat tgttcgccaa aaccgctgcg ctacgccttc
     5161 agcttcttcg gtctgattga cctgctggcg atcctgccgg gcatcctcgc cctgttctac
     5221 gccgacgcgc agtacctgct gatcatccgg gtaatacgca tgctgcgggt gttccgcgtg
     5281 ctcaagctca gcccctacct tagccaggcc aactttctgc tgaccgcgct gcgcggcagc
     5341 aaacagaaga tcatcgtgtt cttcgcctgt atcgccacca tggttacggt gttcggcacc
     5401 ctgatgtacg tgatcgaagg ccccgcccac ggttttacca gcattcccaa gggcatctac
     5461 tgggccgtgg ttactctgac caccgtcggc ttcggcgata tcgtcccgaa aaccccgctg
     5521 ggccaggcgc tgtcgaccct ggtgatgatc accggttact caatcatcgc cgtgcccacc
     5581 ggtatcttca ccgccgaact ggccaacgcc atgcgcggcg acggccagat gatgcatgac
     5641 tgcccaactt gcgccaaaag cactcatgag cacagcgccg ccttctgcag ccgctgcggc
     5701 aacccgctgt ttccaaggca aaccgacaaa ccctgagtaa tcagcggcaa tagcaaggct
     5761 cgtatcgatt gcgcccagtg aagcccagca gattgcagct gtctcgttgg gcttcgtcgc
     5821 attgcacccc aacggcgccc cgcccgcccc aacctctccc tgcctaagcg cccaatagcc
     5881 actggccacg ccaacaggct ttggcacgcg gttgattggg cttcgcgcac aaggccgccg
     5941 aaaaaacagt cgaacctcag cggcgattcg atagtcacag ggaaacaacg gacgtggtcc
     6001 gcaaccgcgg ccagcaatgc acggggcagc tatgccgcta ctcaaactgc tgcacttcat
     6061 tgccttgatc ggctggtgtg gcgctctgct ttaccttccc gcgctgatcg cggccggcac
     6121 ccgtagcaac gaggcgttgt tctaccgcga tcacgctcat ctgacgcgca tggtcttcac
     6181 cctagtcgcc acaccagcgg ccttgttggc catcggctcg ggcacgctct tgtttgtgcg
     6241 cgagtcggtg ttcgatccct ggctgattct caagctcagt gctgtcgccg gcatggtgct
     6301 gtgccatgcg ctgtgtgggg tgctgattct gcgtatcgaa cgcaccccgg cgcgcgccat
     6361 tggccaccac tgcctgggta taggcgcact gctggtcgtg ctgatcaccg ccacgctctg
     6421 gctggtgctg gctaaacccg cattctgaag gaggaatcga cgcgatctat gaacgtgccc
     6481 gcccccagcg ccgccgcggc gaaatcagag gttcacctca acgccgaccg cttgttcata
     6541 gaccaggcgc ccgccgaggt atgcggccag ggcaatcaac ccggcggtca gcagcgacag
     6601 gtacaaacac cagagctgca tgccatccag cccctggtag cgcagcaacc agttgagcgt
     6661 ggccagcgag agcatcatca cggcgatgat tgcatggcac caggccgtga tcttcaggcg
     6721 aatgcgctca accgtgagca ggtctatcaa gccggccagg cttgccagcc aaccacccag
     6781 ggaaccgata ccggccaacc agaggctggc acgccaccag aacgggtcat cgctccacag
     6841 gtaggccagg tcactggcga ccaaccccag caacgccgct acggggaagt ggatcatcat
     6901 cgggtgcaac gggtgaccgg caatggcggc gcggctgaaa atcgaatcgt ttttatcagt
     6961 catccgggag gccttgatca ggttggcggg ttctgcgctc gtcgcgcgcc cttagtgcaa
     7021 ttgaccgcgg cggctctggc agttcccatt ctttctgctt gcagtgggcc gcaatcggcg
     7081 ctcgatccgg ctgggcccat ggcccgcgag gtggcgctcc tatggtggct gatgtgcggc
     7141 tttgccggct tggtgctgct ggtggtcagt gcgctgtgga tttacgccat gcgccgcacg
     7201 ccgcaggaaa tcacgcccga acaggccaag cgaatcaacc tgcgctggtt ggtcggcggc
     7261 ggcctcctgc tgcctgggtt gagcatcacc gtgctgctgt tcttcggcgg ctccatcggc
     7321 gagcgcatgc tgccgttgcc gctgctgggc gaacagccgc tgcgcatcga gatcaccggt
     7381 catcagtggt ggtgggaggt gcgctacccg gacagcggcg tggtcaccgc caatcagttg
     7441 catattccgg ccggccgccc ggtggatctg caggtcagca gcgccgatgt gatccattcg
     7501 ttctgggtgc cgcgcctggg cggcaagatc gacatgatcc ctggcaggca caaccgcatc
     7561 cgtctgcagg ccgacgagcc cgggatctat cgcgggcagt gctcggaatt ctgcggcacc
     7621 cagcacagcc atatgattct gcatgtacag gcccacgccg aggacggctt taacgcctgg
     7681 ctcagtggcc ggcagaacgc acagatcagc ccacctgcgg gcgctgcggg ggaaatctat
     7741 gccacgcgct gcggcatctg ccaccgcgtg gccggggtca gcgccggcca gcgcgcgccg
     7801 gacctgtccg acctgggttc gcggccgacc ctgggcagtg gcgtgctgac caatgaaccc
     7861 ggcgccatcc tcacctggct gcgcgaacac caacgcctga aaccgggcaa tgccatgccg
     7921 ctgcatgacg atatcgaccc cgcgcatctg cacaccatcg ccacctggct agagggccta
     7981 gaaccttgag cagccgaaac cacgcgccgg ccaccgaccc ggatcagttg cacgaccagt
     8041 tcaatgcggt gtggggcaac ccaaagggct ggcgcgccct gaccatcgtc aaccacacca
     8101 ccatcggcct gcgctttatg ctcaccggcg cggtgttctt tctcttcggc ggcctgctgg
     8161 cgatgctgat ccgcacccag ctagcgctgc cgaaccatga gtttatggag ccggacgtct
     8221 acaaccaggt gttcaccatg cacggcacgg tgatgatgtt cctgttcgcc gtgccaatga
     8281 tggagggcat ggcggtttac ctgatcccga agatgcttgg tgcccgcgac ctggtgttcc
     8341 cacggctatc agcgctgggt tactactgct acctgttcgg cggtcttatc ctgatcacca
     8401 gcctgtttct cgaccttgcg cccaaggccg gctggtttat gtacacaccg ctttccagcg
     8461 ccgcccatac gccggggcca aactcggact tctggctgat cggcattacc ttcgtcgaga
     8521 tatccgcagt cagtgccggg gtcgagctgg tggtgtcgat tctgcgcacc cgggccagcg
     8581 gcatggcgct gcacaagatg ccgatctacg cctggtacat cctggtgatg gcgatgatga
     8641 tcgtggtcgg ctttccaccg ctgattctcg gctcgattct gctggagctg gagcgcgccg
     8701 ccggcatgcc gttcttcgag gtcgcccgtg gtggtgatcc gatcctctgg caacacctgt
     8761 tctggctgtt cggccacccc gaggtgtaca tcatcttcct gcctgcggcc ggcatcgttt
     8821 cgaccctgct gccggtgttc tgccagcgcc cactggtagg ctaccgctgg gtggtgctgg
     8881 gtgtgctgac caccggtttt atcagcttcg gcctgtgggt ccaccatatg ttcaccgtgg
     8941 gcatcccgca gctggctcag ttgttctttt ccgcagccag catgctggtg gcaatcccca
     9001 ccgcggtgca ggtctttgcc tggctggcga ccctgtggca gggcaaaccg gtgtaccacg
     9061 tgccgatgct atggctggtg ggttttctga tcgtgttcgt ctgtggcggc ctgaccggcg
     9121 tgatgctggc gctggtgccg ttcgactggc aggtgcacga cacccatttc gtggtcgccc
     9181 atatgcatta cgtgctggtc ggtggcatgt tctttccgat gatggccggt atctactact
     9241 ggctgccgca cttctccggg cgcatgccct cggtgcgtct aggcaagtgg ggcttctggc
     9301 tggtgtttat cggcttcaac gtcacctttc tgatcatgca ctggaccggc ctgatcggca
     9361 tgccgcggcg catctacacc tatcaaaccg gcctgggttg ggacatcccc aacctgatct
     9421 cctccgtggg cagctttatc atggccatcg gcgttgccac catcttgctg gacatcatcc
     9481 tgcactttcg cttcggccag ccggccaagg tcaacccgtg gcgcgccgac accctggaat
     9541 gggccaccag cctgccgcca agcgcctaca actttgtcag cctgccggcg attcacagtc
     9601 gccacccgct ctgggataac cccgggctgc cggatagcat cgcccgtggc gaacatgagc
     9661 tggcggtggt cgatcatggt cggcgcgaga cctggggcac cgatccgctg accggcaagg
     9721 tacgcgagat cattcacctg cccggcaata gctggatacc gctgattgcc gcgctgttta
     9781 ttgccgcgat gtgcatcagc ctgctggtca aggtctactg gctggccgcc gtattctttg
     9841 ccgctgcggt gctgacgttg ttgcgctgga gctgggaaaa cggcacccac cccctggcag
     9901 cgcccgatgc cagggtccag cccggcgagc ccccgctgca ctcgcgcacc ctggatggcc
     9961 ccggcttatg gggcatgggc gtaacgctgc tggccaatgg cgcgctctac ctggcgttgc
    10021 tgttcggctg gttctaccta tggacggtgg cgccgaactg gcagatgccg cagcacatcc
    10081 cattcaacca gtggttgctg ctggccagcg ccgtgctgtt gagcctggcg acctggtggc
    10141 aggcaagggc gatcaagcgc ttgcgccacg gcaatgctca aggcttacgc ggccatctgg
    10201 ccctggttgc ctgcctgggt ctgggccaat ccggcctgct gctgtggacg ctgctcggcg
    10261 gcgagctggc accgcgcgca accgcccacg acgcggtgat tgtggtggtg ctgatctaca
    10321 gcctgctgca ctgcgctcta gcggccactc tcagcgccct gcaggccata cgcgtgcact
    10381 gcggctatgt cggtgcccac gcgccttatg aacctctggt ggtcggccat ctctggtact
    10441 acaacctcgg cgtgctgtgg agcagctact tcgccctggt actgtttccg agcacctggg
    10501 gaggcagcgg atgaaaggcc tgcgctcgcc ctatcacccc ggccatatcc cactcggcct
    10561 ggtgttctgg agtctatggt tcgtcgccct ctacggcggc ctgtccgtgg cctgcgcgct
    10621 agccccgcct gaacccgagc aaggcccatg gaactggctt aatgctctgc ttggcgtaag
    10681 cgccctgctt actgcagcgg cgcttgctgc gctggccatc tggtgttggc gcgctgggaa
    10741 accgcagccc tcgccaggcc accagcgctt cgtcgccttg ctcgccgccg gcacctatgg
    10801 ggtggccaca gtagccaccc tggtcatcgc cttgcccatt ctgagcctgc caccatgcat
    10861 gtaataaccg ccctctgcct gttgctggtg atgccaacag cgcaggccca cggcctgctg
    10921 gatcaccaac tcaatttgcg cgtgccactg ctcctcagcg ccctgatgct ggccaccgcc
    10981 tgggtacttt acggcttagg cgcacgacgg gtacggcccc acggcctgga agcgctgtgg
    11041 ctacacctgg ccatgttgat cgcggtactg gcggtattcg gcccattgga cgactgggcc
    11101 gagaccagca ccagctggca tatgcttcag cacatgctgt ttatgctggt aatcgccccg
    11161 ctctgggcac tggccaggcc cttgccgcaa tggcgcgcag tgtgcgggcc gctgctgcaa
    11221 ccgctatggc gcggcgtatt gcgggccggt cgttaccccg tgccgctggc catgctgcac
    11281 ggcgcgctga tctggatctg gcatacgccc agcctgtata tcctggccct ggagaatctc
    11341 tggtcgcata tgctcgaaca cgccagcttc ctgttcagcg cctggctgtt ctggtgggcc
    11401 tgcctgcgcg cgccaccgcg gcaggtgccg caggcgctgc tggcgatact gctgaccctg
    11461 atgcacaccg gcctgctcgg tgccctgctg acgttcggca cgagctcgtt ttatggggcc
    11521 gagcgtgacc tggccgatca gcaactggcg gggctgttga tgtgggtgcc cggcggcctg
    11581 gtctacctcg ccgctgccgg ctggatcacc tggcgctggt tggcgcgtag ctggcggcac
    11641 cagcaaagcg cacgggcatc gaagggctca gcctgacgct ttcggcgacg atgactcgtt
    11701 ccactcgctg cgcttcagcc gcgttcgatt ggcgtagaag tcaactcaaa tggactattg
    11761 ctgcgccgct ggttgcggtc ttcgcgcggc gtagcgccga agaaattacg gtaggcgctg
    11821 gagaagtgcg gcccggatga gaaaccgcag gacagaccga tctggatgat cgatttgctg
    11881 gtctgcatca gcagctggcg cgccttgttc aggcgcagct ccaggtagta ctggctgggc
    11941 acgcgattga ggtattgctt gaagatccgt tccagctggc ggcgcgatac gcagacatgc
    12001 tgggcgattt cgtcggtggt cagcggctct tcaatattgg cttccatcag caacacggcc
    12061 tgagtcagct tggggtggct ggagcccaga cggttctgca acggaatacg ctggcgctcg
    12121 ccaccttcgc ggatacgctc gactaccaac tcctcggata ccgcaccggc caattcggca
    12181 ccgtggtcac gggccaacaa ggccagcagc agatcaagca cggccaggcc gccacaggcg
    12241 gtcaagcgat cacggtccca gtcgaacaga tggctggtgg cgatgacctt ggggaagcgt
    12301 tcagtgaaat catcctgcca gcgccagtgc accgaagcac ggtagccatc gagcaggccc
    12361 agctgcgcca gcggataaac cccggcggca atcgcaccga tcacgcagcc
//
LOCUS       DASRUM010000016        21687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1179904, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000016 DASRUM010000000
VERSION     DASRUM010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 21687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21687
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179904"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..626
                     /locus_tag="VFF22_01135"
                     /pseudo
     CDS             1..626
                     /locus_tag="VFF22_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042650166.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(682..1254)
                     /gene="efp"
                     /locus_tag="VFF22_01140"
     CDS             complement(682..1254)
                     /gene="efp"
                     /locus_tag="VFF22_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZX15459.1"
                     /translation="MKTAQEMKPNSVALIDGQPWLIQKAEFTKSGRNSAIVKMKLKNL
                     LSGSSTETVYKADDKMEPVILDRVDVTYSYYSEPNYVFMDQEFNQYELNKDDLESVMP
                     YIVDGMTDICEAVFFEGKVVSVDLPTTIVRQITYTEGSARGDTSGKVMKPAKLSNGTE
                     IKVADFCNIDDWIEIDTRTGEYKSRAKAPV"
     gene            complement(1301..2434)
                     /gene="earP"
                     /locus_tag="VFF22_01145"
     CDS             complement(1301..2434)
                     /gene="earP"
                     /locus_tag="VFF22_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HZX15460.1"
                     /translation="MRVTWDIFCSVVDNYGDIGVTWRLARQLVAEHGLAVRLWVDDLG
                     AFVRLCPQADAQALRQVQQGVDVCLWRAEWQAVESADVVIEAFACELPASYIAVMAQR
                     SQKPLWLNLEYLSAEDWVEGCHGLPSLQSNGLSKFFFFPGFTAGCGGLLRERELLVQR
                     QAFQADSGAREVFLRSIGVTPLPGSRLISLFAYENAALASWLDVLAVDAQASQLLVPE
                     GRILADLQAWLGCTGMRAGDVQQRGQLHIQVLPFIPQDQYDRLLWSCDFNAVRGEDSF
                     VRAQWAGRPLLWHIYEQANNAHLEKLDAFLALYKEGLSADGQRALVELWHAWNTGQSV
                     KESWNQLLQGWPEQQAYAEQWCLQRASQADLAEALVQFYLNWL"
     gene            complement(2542..2631)
                     /locus_tag="VFF22_01150"
     tRNA            complement(2542..2631)
                     /locus_tag="VFF22_01150"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2595..2597),aa:Ser,seq:gga)
     gene            complement(2724..2951)
                     /locus_tag="VFF22_01155"
     CDS             complement(2724..2951)
                     /locus_tag="VFF22_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15461.1"
                     /translation="MAGLIRLIRCWRAALALLLLAGMLWLASNQHSQSPAGVLGLFGF
                     GLFISLGFILFGQQRFEPISAKPSGEDQHLA"
     gene            3136..3825
                     /locus_tag="VFF22_01160"
     CDS             3136..3825
                     /locus_tag="VFF22_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZX15462.1"
                     /translation="MSDLISYQLDDGIATLTLCNGKVNAISPDVITAFNAALDRAVQD
                     KAIVIITGQPGILSGGYDLKVMTSGPQNAIDLVASGSTLARRMLAHPFPIIVACPGHA
                     VAKGAFILLSSDYRIGVEGPFSIGLNEVQIGMTMHHAGIELARDRLRKSAFHRSVING
                     EMFDPQGAVDAGFLDKVVPAEQLHATALAVAQQMKKINMTAHKNTKLKVRKALLEALD
                     QAVELDKKHMG"
     gene            3993..5876
                     /locus_tag="VFF22_01165"
     CDS             3993..5876
                     /locus_tag="VFF22_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX15463.1"
                     /translation="MKIAHKVGLAAAIVLFLTTSLLSLAQVSQVRETLHNQVAASIAE
                     SSNALARQIENWLNAKLKLMDLMSQSIDSNYSPEETQRIVNSPMLKNEFILVFGTLQS
                     DGKPIKNTDEWKPAPEWDGRQRPWYVTGKAGNQAVLTEPYVDSTTGEILISAVTRITN
                     AGEFLGVLGGDIRLKTVADAVNTLDFNGAGYAFLLTRNGNIISHPNTELNGKSYSELF
                     GGQSPALNKELQETSSGDKDLLVSFTPLPNLKGMDWYIGVVLDKSIVMAEANTLSWRA
                     VIGTVLGVIISLIVLGVLMNSLLKPLDHLHNSLREVNSGEGDLTRRLPATGNDEIALV
                     SKEFNSFLQSLQSLISEVMGSSRQVRESTTLTSSEANQAANRLQQQLQELDQLATAMQ
                     EMASTAEEVARNAQAAAQAALAANQETENGVRVVSRSTEAIKNLAEEMDETSHAINEL
                     AKLSHNIESILSVITSIADQTNLLALNAAIEAARAGESGRGFAVVADEVRSLASRTQQ
                     STQEIRQMIDQLQSGVKQAESRMQQSRDTASKTAEEAGAANDMLGRISDAITRINDMN
                     LQIATAAEQQSATTEEINRNTTNIRDISHEVAAGAEEQVRQCAVMVEQVGQQDHLLGR
                     FKV"
     gene            5939..6799
                     /locus_tag="VFF22_01170"
     CDS             5939..6799
                     /locus_tag="VFF22_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX15464.1"
                     /translation="MRILVTWLLCLAMQGIAQAQTPISVTVLCDASYPPYSYAENGEA
                     KGLYSDILRAAFAHMPNYHVSIRPVPWPRGLAALEKGTSFALFPPYHRPVERPWMDYS
                     RPILEESVVAFVRAELAQQRAIEDFPAAYAGLRIGLNRGFNIIAAPSYKTMLAAGEVK
                     QSYATDNRTNLLQLRRKRIDVYINDRLSILWELQQMRSEGLFASSGLDWLVEGPKLSS
                     EHGHLGYTRINEQAYPYKQDFMQQLDQALLALEQDGSITRLAQRYSSIDLLPATGKGA
                     DEDATSGNAP"
     gene            6870..7592
                     /locus_tag="VFF22_01175"
     CDS             6870..7592
                     /locus_tag="VFF22_01175"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HZX15465.1"
                     /translation="MLFLLRMLAMGVHFILAGILGLLLGLCRPFNPDNSRLCARLYSI
                     PAQWLLRWKLKTDVHSLEHQHSCVIVANHQSNYDLYVLGRVVPKRTVSIGKKSLKWVP
                     FFGQLYWLAGNVLLDRGNAIRAKQAMLTTTDTLKHKDTSIWVFAEGTRNLGKGLLPFK
                     KGAFQMAIAAGVPIIPVCVSSYVNHLQLNRWNSGEIMIRSLPAIPTAGLSQDDLPQLL
                     ETCRTQMQQCIDAMDQQLSQSA"
     gene            7735..8706
                     /locus_tag="VFF22_01180"
     CDS             7735..8706
                     /locus_tag="VFF22_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015885387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HZX15466.1"
                     /translation="MGRVVAAAVYSNGKKVTNISLDEGKPWSKKPGHFVWIGLHDPCA
                     NELISLQQQFDLHELALEDALQRHTRPKLETFGDALFLVLYSPILVNDELTFVETQLF
                     AGSGYIISARYGESAPYSQVRQRCEARPLLLEHGEDFVLYALLSFVIENYRPLMDNYH
                     VELEEVEQHVLKNPLSQADVERIQGLRRDLLRLRRYIGPLAEICKELQRLDFPFIDKN
                     MRPYFRDVAIHVNRLLEDLTGLREMADHAIEIGLLLESSRQSVTQRKFAAWAAILAFP
                     TAVAGIYGMNFQNMPELAWQYGYFGVLGLITTGCVGLYVSFKRSGWL"
     gene            complement(8927..9655)
                     /locus_tag="VFF22_01185"
     CDS             complement(8927..9655)
                     /locus_tag="VFF22_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidotransferase"
                     /protein_id="HZX15467.1"
                     /translation="MPLQICILETDVLRPELVDQYDSYGRMFEQLFARQPIAAEFKVY
                     NVMEGHYPPDSERYDAYLVTGSKADSFGSDAWIQTLKTYLLERYNRGDKLLGVCFGHQ
                     LLALLLGGKAERAVQGWGVGIHQYRMAGKPTWMSPDLEDLTLLISHQDQVTQLPLNAT
                     LLASSEFCPIASYCIGDQVLCFQGHPEFVHDYSRALLDLRQQHIGEPAYSKGIDSLQH
                     DHQGHTVAEWMMRFVAQGRAGLAE"
     gene            9877..10011
                     /locus_tag="VFF22_01190"
     CDS             9877..10011
                     /locus_tag="VFF22_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15468.1"
                     /translation="MTSFSAIASLLAASAFTSNHSASSQRQAEKVQAAQLKADQNAGK
                     "
     gene            10038..10235
                     /gene="ptrC"
                     /locus_tag="VFF22_01195"
     CDS             10038..10235
                     /gene="ptrC"
                     /locus_tag="VFF22_01195"
                     /inference="COORDINATES: protein motif:HMM:NF041597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system co-regulatory protein
                     PtrC"
                     /protein_id="HZX15469.1"
                     /translation="MSIAQSLSNQNVYGVTYATVDDSGIHFESELAIQLSDGTLTTLR
                     MPTHLSERQAIQQLVCGRQAC"
     gene            complement(10239..10637)
                     /locus_tag="VFF22_01200"
     CDS             complement(10239..10637)
                     /locus_tag="VFF22_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008027953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HZX15470.1"
                     /translation="MQILKVCALVLAGFWAAPSWADDCANAMDQFTLNQCAAAGYAAQ
                     DKRLNQLYGDYRKRLDDGQKQQLKEVQLAWIKFRDLACAFESASVEGGSAYPMVLNGC
                     LTQKTVARVRELEQLAVCEEGDLSCPMPAQ"
     gene            complement(10682..11161)
                     /locus_tag="VFF22_01205"
     CDS             complement(10682..11161)
                     /locus_tag="VFF22_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15471.1"
                     /translation="MKQGLIGVLLMALLGTAQAADERVYLVALANLGGSNLAQVVFLH
                     EPEITQLEVCEEARLKGIRERDWARYHHIFMSHRMKGYTVQLQYRCVVSEQPIDSWYD
                     KARYDYAYLVEVDATARMHLRQTTSLAQCTSTLQSMPQAQRQQAYCAKANQKLLAAQ"
     gene            complement(11271..13415)
                     /locus_tag="VFF22_01210"
     CDS             complement(11271..13415)
                     /locus_tag="VFF22_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZX15472.1"
                     /translation="MTDAIRYEKGQDNIVVLTMDMPGQSANTMNAVYREAMGKIVERL
                     EAEKDSIAGVIVTSAKKTFFAGGDLNELIKVTKADAPAFYQMILNIKGQLRRLECLGK
                     PVVAAINGAALGGGWEIALACHHRIALDSSSIQLGLPEVTLGLLPGGGGVVRMVRLLG
                     LEKALPYLAEGKKVRPDAALKAGLIHELASDKDDLLARARAWIAANPAPKQPWDVAGY
                     KIPGGTPSSPNVAQMLAIAPSVLRDKTKGCFPAPEKIMCAAVEGAQVDFDTAQIIEAR
                     YFTELTTGQVAKNMIGTFWFQLNEINAGGSRPQGFEPYVTKKVGVLGAGMMGAGIAYV
                     SAVAGIEVVLKDISIEAAEKGKDYSAKLLEKKVGRGQMSADKRDGILARIKPTVSDAD
                     FEGCDLIIEAVFEDRDLKANVTAAAERAALSDAVIASNTSTLPITGLANAVQKQDKFI
                     GLHFFSPVDKMPLVEIIKGEHTSDETLARGFDYVMQIKKTPIVVNDSRGFFTSRVFGT
                     FTNEGLAMLGEGVSAAMIENEARKAGMPVGPLAISDEVSMSLMNHIRQQTIKDLAAEG
                     KQIPEHPAFAVIDMMLGEYKRPGKVAGAGFYDYPAGGKKHLWPELKARFEKPDAQISQ
                     IDVRDRILFIQAIETVRCVEEGVLKSVADANIGSIMGIGFAAWSGGALQFINQYGVKD
                     FVARAQYLAEQYGERFLPPALLLEKAAKNELF"
     gene            complement(13579..14784)
                     /locus_tag="VFF22_01215"
     CDS             complement(13579..14784)
                     /locus_tag="VFF22_01215"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZX15473.1"
                     /translation="MTEAFIFDAVRTPRGKGKKDGALYSVKPVDLIAGLLKALQARNN
                     LDTSQVDDIVLGCVTPVGDQGADIAKTAAMVADWDVSVSGVQLNRFCASGLEAVNLGA
                     MKVRSGFEDLVVVGGVESMSRIPMGSDGGAWVMDPATNMHTHFTPQGIGADLIATLEG
                     FSRTDVDSFALRSQQKAARASKDGSFNKSLIPVADQNGIVLLDHDEFIRGESTLEGLG
                     KLKPSFEMMGQMGFDSTALRVYSHVEQIKHVHTPGNSSGIVDGSALMLIGSEAKGKEL
                     GLKPRARIVATAVTSTDPTIMLTGPAPATRKALAKAGLNVNDIDLFEVNEAFASVVMK
                     FMKDMGVSEDKVNVNGGSIAMGHPLGATGCAILGTLLDELEKRKLRYGLATLCVGGGM
                     GIATIIERI"
     gene            15459..15818
                     /locus_tag="VFF22_01220"
     CDS             15459..15818
                     /locus_tag="VFF22_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZX15474.1"
                     /translation="MRTLLATLSLLISLPLLAGEAELSAAVVALQSPESVLIDVRTAD
                     EFAAGALPGAEQIEHEQIASRINAIAPDKDTPIILYCRSGRRSGIAEENLRAMGYSNL
                     INAGGYDELKLALESQD"
     gene            15815..16627
                     /locus_tag="VFF22_01225"
     CDS             15815..16627
                     /locus_tag="VFF22_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008017651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZX15475.1"
                     /translation="MNKRTLCIALLALLPFSNLHAAELTLELGHGSQRLSSAQLLAHP
                     QAQNVEISDDVSYKRSMRYRAVPLAALLEGVEPGDHLQLVASDGFAAELSAAPALSQT
                     GSQAWLAIEDPAHPWPALGNGKPSAGPFYLVWQNPAASQIGPEQWPFQLATIRKLAPV
                     AQRFPQLLPAADASPAVQAGFAQYQKNCMACHRLNGAGDSQFGPDLNIPHNPTEYFSG
                     DFLTLYIRDPQSLRRWPQGKMPGFAVEVLSDKELGELVEYLGHMVGRKVQPL"
     gene            complement(17120..17917)
                     /locus_tag="VFF22_01230"
     CDS             complement(17120..17917)
                     /locus_tag="VFF22_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016973635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZX15476.1"
                     /translation="MRLSISHDTTYHYEDQVRTSIQYLRMTPHDSERQQVLSWQLTLP
                     RPVRAQLDPYGNILHVLTMDEPHESIVIGARGQVEIDEAREAEHESQSALPFLRFTRL
                     TEADEALREFAKLQSRSRPDRSGLIDLMHALNAHIRYQPGSTAVETSASEAFAGGKGV
                     CQDHAHAFLACARSLGVPARYVSGYLFTDSEDHLASHAWAEAWLDDAWYSFDVTNCLA
                     KPERHLKLAVGLDYLDACPVRGMRRGGGIEQMHAQVEVAPLVRVQQQ"
     gene            complement(17914..18864)
                     /locus_tag="VFF22_01235"
     CDS             complement(17914..18864)
                     /locus_tag="VFF22_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HZX15477.1"
                     /translation="MLSRTASDLYWMSRYLERAENLARMLEVSYSLSLMPQDGRGDGL
                     EELALPLLITGTLDDYLERHGPLHAERMLHFFALDASNPASIYCCLQAARSNAHAVRG
                     RITADMWENINATWLEIHVIAQQGLSRYGISRFCEWVKERSHLFRGATFGTIMRGDPY
                     RFIRLGTFIERADNTLRLLDARYEMLGEDIDEIDGRPARSYYQWTALLRALSSFEAFA
                     EIYRGSPGTRKVSELLLLRADVPRSLRACMDELSLMLASLPGDNGRPAQRLAAELEAR
                     LRYTSIEEVLDEGLHAWLTDFILLVRQLGQAIQSSYLEVA"
     gene            complement(18858..20276)
                     /locus_tag="VFF22_01240"
     CDS             complement(18858..20276)
                     /locus_tag="VFF22_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HZX15478.1"
                     /translation="MARTFYDEMYDASGAVRPHYQAFARWLADTPDELLAQRRREADL
                     LFHRAGITFTLYGDDQGTERLIPFDIIPRSIPASEWRIVERGCSQRVQALNMFLADLY
                     HDQRIIKAGIIPAEQVLANEGYQIAMQGLNLHRDLYAHIAGVDLVRDGDGSYYVLEDN
                     LRTPSGVSYMLEDRKMMMRLFPELFAAQRVAPIDHYPNLLLDTLKSSSPLDNPNVVVL
                     TPGRFNSAYFEHAFLSREMGVELVEGADLFVRDDKLFMRTTAGAKQVDVVYRRLDDAF
                     LDPLAFNPDSMLGVPGLLACYRSGNVVLANAIGTGVADDKSIYPYVDEMIRFYLSEEP
                     ILKNVPTFQCRKPAELSHVLANLEHLVVKETQGSGGYGMLVGPAATKAEIEDFRARLI
                     ARPEAYIAQPTLCLSTCPTFVERGIAPRHIDLRPFVLSGKETRIVPGGLTRVALREGS
                     LVVNSSQGGGTKDTWVVEDSSC"
     gene            complement(20494..21201)
                     /locus_tag="VFF22_01245"
     CDS             complement(20494..21201)
                     /locus_tag="VFF22_01245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HZX15479.1"
                     /translation="MTDILIFTHIDYCPPAHLGKVLEQAGHSFTVMRADLGELDGIDL
                     DRPKAVAIMGGPMSVNDPLPWLQTEVAALQHFIRRDIPLIGHCLGGQLLAKALGAEIS
                     RMPYTESGWQPLSRLDQAAPSPWLQHLPEQFPIFQWHGDSFAIPKGAQPLLSSPWCSN
                     QAFAWGDKVLALQGHPEMDEALVRQWLGDWHHLLDETQPSQQSRAQMLEELPAKVAEL
                     NRVAEGFYRHWLQLAGL"
     gene            complement(21313..21648)
                     /locus_tag="VFF22_01250"
     CDS             complement(21313..21648)
                     /locus_tag="VFF22_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HZX15480.1"
                     /translation="MSTALHDDVSNGVLRRMKEGGFDFASFHPIEFYALFPDEERARM
                     ATRKFRGESLHALVTERDDGVWHLQVSKVMFATYHGIDDFEHDLESVVAPLGGKLDGW
                     GVTQEVRQP"
BASE COUNT         4566 a         6767 c         6270 g         4084 t
ORIGIN      
        1 gtgcggcatc agcaagaaca cagcttttct ctggcgccac cgcttcttgg cctgcgcggc
       61 ccagcaccat gcagcacatg aaacgggcat tgttgaggtc gatgagacgt tttttctgga
      121 gtccttcaag gggcagcgca atctgccaag agcccccccc gcaaaagggg cggcgtgggc
      181 aaaacgcgcg gcacgggcaa ggatcagatt cctgtcatgg tggtgcgtga tcgcgagggc
      241 ataccgcaga cttcaagctg gaaaagctcg atgcagagca tgtgcgagct gccttactgc
      301 cgctgatcga ccgggaagcc gtactgtgca gtgatggcgc tgcggtctac gcaaactttg
      361 cccggaccca gggcattact caccatgtag ttcactcgcg tcctggcgag cgggtaaggc
      421 agggcgcttt ccacattcag aacgttaatg cctaccacag ccgcctaaag agctggatga
      481 ctcgctttca tggtgtcccc acccgctacc tcccgaatta cctgggctgg cgccgcatgc
      541 ttgagcgtta tcagcagcgt attcagccgg ttcactgtat ccaggaggcg gtagggagaa
      601 ccatgcaaca cgctattggg acatagccat aaaaaagccc gactcaaggt cgggctattt
      661 ctcacgcgat taaagccgat cttagaccgg agctttagcg cgggatttgt actcaccggt
      721 acgggtgtcg atttcgatcc agtcgtcgat gttgcagaag tcagcgactt tgatctcggt
      781 gccgttgctc agcttggctg gcttcatcac cttgccggaa gtgtcgccac gggcggaacc
      841 ttcggtgtag gtgatctggc gaacgatggt ggtcggcagg tcaaccgaaa ccactttgcc
      901 ttcgaagaat acggcttcgc agatgtcggt catgccatca acgatgtacg gcattacgct
      961 ttccagatcg tctttgttca gctcgtactg gttgaactct tggtccataa agacgtagtt
     1021 cggttcgctg tagtacgagt aggtcacgtc tacgcgatca aggatcaccg gctccatttt
     1081 gtcgtcggct ttgtacacgg tttcagtcga ggagccgctc agcaggttct tcaacttcat
     1141 tttaacgatg gcgctgttac gaccggattt ggtgaattcg gccttctgga tcagccaagg
     1201 ttggccgtcg atcagggcca cactgttggg cttcatttct tgtgcggttt tcatacgaat
     1261 atccgggttt ggatggaatt acaaaattct tggccgcgta tcatagccaa tttaggtaaa
     1321 actgcaccag cgcctcggca agatcggcct gagaggcccg ttgcagacac cactgctcgg
     1381 cgtatgcctg ctgctctggc cagccttgta gcagctgatt ccagctttct ttgaccgact
     1441 gaccggtatt ccaggcatgc cacagctcga ccagagcacg ctgcccatcc gctgataagc
     1501 cctccttata gagagccaga aaagcgtcga gcttttccag atgagcgttg ttcgcttgct
     1561 catagatatg ccagagcaac ggccgcccgg cccattgcgc acggacgaag gaatcctccc
     1621 cgcgaaccgc attgaaatca caactccaca gcagccggtc gtactgatcc tgcgggataa
     1681 acggcagcac ctggatatgc agctgaccgc gctgctgcac atcccccgcg cgcatgcctg
     1741 tgcagccgag ccaggcctgc agatccgcca gaatgcgccc ttccggcacc agtaactggc
     1801 tggcttgcgc gtcgacagcc agcacatcca accagctggc cagcgcagcg ttctcgtagg
     1861 caaacagaga aatcagccgc gacccaggca acggcgttac gccaatactg cgcaaaaata
     1921 cctcgcgggc accgctatcc gcctgaaacg cctggcgctg caccagcagc tcgcgctcac
     1981 gcagcaagcc gccgcagcct gcagtgaatc cgggaaagaa gaagaacttc gacaagccgt
     2041 tggactgcaa tgacggcaag ccgtgacagc cctcaaccca gtcttcggcg ctcaagtact
     2101 caaggttgag ccagagcggt ttctgcgaac gctgcgccat cacagctata taagaagccg
     2161 gcaactcaca ggcaaacgcc tcgatcacca catccgccga ctcaaccgcc tgccactcgg
     2221 cacgccatag gcagacatca acgccctgct ggacttgccg caacgcctgc gcatctgcct
     2281 gcggacacag gcgcacaaac gcccccaagt catcaaccca cagccgcacc gccagaccat
     2341 gctcggccac cagctgccgc gccaaccgcc aggtcacccc gatatcgccg tagttatcaa
     2401 caacgctgca gaaaatgtcc caagtgacgc gcatgcgctg ctccgttatg tactgagcac
     2461 accttaaagg cggctgaacc gtcagcaaac gtcagaaacg aaaaagcccg acacgtggtc
     2521 gggctttttc gtcaattcgt atggcggtga aggagagatt cgaactctcg atacgatttc
     2581 tcgtatacac actttccagg cgtgctcctt aagccactcg gacacttcac cgggtatcga
     2641 caagcattgc tgtctgtcga ggcgcgctaa tctagccgaa cagcttgcca aaggcaaaat
     2701 cttttttcaa aaaattcatg cgcttatgcc aggtgctggt cttcacccga gggcttggct
     2761 gagatcggct cgaagcgttg ctggccgaac aggataaagc ccagggagat aaataggccg
     2821 aagccgaaca gccccagcac accggccggg ctttggctgt gttgattgct cgccagccag
     2881 agcatgccgg ccaacagcag caaagccagt gctgcgcgcc agcatctgat aagtcggatt
     2941 aaaccagcca tatcggtcac tcctttatag agtgccgacc ttaagtgcac agagatcggg
     3001 cagcccaatt ggcgatcttg atcgccgcca tagcccaaac tgccgcaatc gctgactagc
     3061 cagtcagtca tcgtgcttta cctgaccggt gcggatgagt aacgtctgct gcacttcagc
     3121 acaaggaacc cgaccatgag cgacctgatc agctaccaac ttgacgatgg cattgccacc
     3181 ctgaccctgt gtaatggcaa ggtgaatgcc atctccccgg atgtgatcac tgccttcaac
     3241 gcagctctgg atcgcgccgt gcaggacaag gccatcgtga tcatcaccgg ccaaccgggc
     3301 attctttcgg gcggctacga cctcaaggtg atgacctccg gcccgcagaa tgccatcgac
     3361 ctggtggcgt ccggttccac cctggcgcgc cgtatgctcg cacacccctt cccgatcatc
     3421 gtcgcctgcc caggccacgc ggtggccaag ggtgccttta ttctgctttc gtctgactac
     3481 cgcatcggcg tcgaaggccc gttcagcatc ggcctcaacg aagtgcagat cggcatgacc
     3541 atgcaccacg ccggcatcga actggcccgt gaccgcctgc gcaaatcggc cttccaccgc
     3601 tcggtaatca acggtgagat gttcgacccg caaggcgcag tggatgccgg cttcctcgac
     3661 aaggtggtgc cggccgagca actgcacgcc accgccctgg cggtggccca gcagatgaag
     3721 aagatcaaca tgaccgcgca caaaaacacc aagctgaaag tgcgtaaggc gctgctcgaa
     3781 gccctcgatc aggcggtcga gctggacaag aagcacatgg ggtaaagccc cagcacaccg
     3841 cgaagcccgg cctatgtgcc gggcttttgc atttaatggc aaaacaatgg caaacccgca
     3901 agcaatctgg ccaggctgta gtacttttaa caggtaaggc cagggagtga ggcactcacc
     3961 gatctattcg aaattgccgc ctggagcgtg ttatgaagat tgcccacaag gttggtctgg
     4021 ccgcggccat cgtcctgttc ctcaccacca gcctgctgtc actggcgcaa gtcagccaag
     4081 ttcgtgaaac cctgcataac caggtggcgg caagcatcgc agagtcgagc aacgcgcttg
     4141 cgcggcagat cgagaactgg ctcaacgcca aactcaagct gatggacctg atgtcgcaaa
     4201 gcatcgacag caactacagc cccgaagaaa cccagcgcat cgtcaacagc cccatgctga
     4261 agaatgagtt catcctggtg ttcggcaccc tgcaaagcga cggcaagccg atcaagaaca
     4321 ctgacgagtg gaagcctgcg ccggaatggg atggccgcca gcgcccctgg tacgtcaccg
     4381 gtaaagccgg taaccaggcc gtgctcaccg aaccttatgt ggactccacc accggtgaaa
     4441 tcctgatttc tgccgtcacg cgcatcacca atgccggcga gtttctcggc gtgctgggtg
     4501 gcgacattcg tctgaaaacc gtggccgatg cggtcaacac cctggatttc aacggcgccg
     4561 gttacgcctt tctgctgacc cgcaacggca atatcatttc ccaccccaac accgagctga
     4621 acggcaaaag ctatagcgag ttgttcggcg gtcagagccc ggcacttaat aaagagctgc
     4681 aggaaaccag cagcggcgac aaggacctgc tggtgtcctt taccccactg ccgaacctca
     4741 agggtatgga ctggtatatc ggcgtggtgc tggacaagag catcgtcatg gccgaagcca
     4801 ataccctcag ctggcgcgcg gtgatcggca ccgtgctggg ggtgatcatc agtctgatcg
     4861 tgttgggcgt gctgatgaac agcctgctca agcccctcga tcacctgcac aactcgctgc
     4921 gagaggtgaa cagcggtgaa ggcgacctga cccgccgcct gcccgccacc ggcaacgatg
     4981 aaattgccct ggtctccaag gagttcaaca gctttctgca aagcttgcag agcctgatca
     5041 gcgaggtgat gggcagttcc aggcaggtgc gcgaaagcac caccctgacc tccagcgaag
     5101 ccaaccaggc cgccaatcgc ctgcaacaac aactgcagga actcgatcaa ctggccaccg
     5161 ccatgcagga aatggcctcc accgccgaag aagtggcgcg caacgcacag gctgcggcgc
     5221 aggctgcact ggcggccaat caggagactg aaaatggcgt gcgcgtggtc tcgcgctcca
     5281 ccgaggcgat caagaatctg gccgaggaaa tggacgaaac cagccacgcg atcaacgagc
     5341 tggccaagct cagccacaac atcgagtcga tcctctcggt gatcaccagc attgccgacc
     5401 agaccaacct gctcgccctc aacgccgcca tcgaagccgc gcgcgccggt gagtccggtc
     5461 gaggttttgc tgtagtcgcc gatgaggtac gctcgctagc ctcgcgcacc cagcagtcga
     5521 ctcaggaaat ccgccagatg atcgaccagc tgcagtccgg ggtgaagcag gcggagtcac
     5581 gcatgcagca gagccgtgac accgccagca agactgccga ggaagccggc gcagctaacg
     5641 atatgctcgg acgcatcagc gatgccatca cccggatcaa cgacatgaac ctgcaaatcg
     5701 ccaccgccgc cgagcaacag agcgctacta ccgaagaaat caaccgtaac accaccaata
     5761 tccgcgacat cagccatgaa gtagccgccg gcgccgaaga gcaagtacgt caatgcgccg
     5821 tgatggtcga gcaggtcggc cagcaggacc atctgctcgg gcgcttcaag gtctgagttc
     5881 gcagacaccg ggctgcaccg acgcgtgcag ccagcgccaa ccgataagga gattggacat
     5941 gcgcatactc gtaacctggc tactgtgcct ggctatgcag ggcatcgccc aggcacaaac
     6001 gcccatcagc gttacggtgc tgtgtgacgc cagttatcca ccctacagct atgccgaaaa
     6061 cggcgaggcc aaaggcctgt acagcgacat cctgcgtgca gccttcgcgc acatgccgaa
     6121 ctatcacgtg agtatccgcc cggtgccctg gccacgcggg ctggcggcgc tggaaaaagg
     6181 cacaagcttt gccctgttcc cgccctacca ccgccccgtg gaacgaccgt ggatggatta
     6241 ttcgcggccg attctggaag aaagcgtggt ggccttcgtc cgcgccgagt tggctcagca
     6301 acgtgccatc gaagattttc cggcggccta cgccggcctg cgcattggcc tcaaccgcgg
     6361 tttcaatatc atcgccgcgc ccagctacaa aaccatgctc gcggcgggtg aggttaaaca
     6421 gagctacgcc acggacaacc gcaccaacct gctgcaactc aggcgcaaac gcatcgatgt
     6481 gtatatcaat gatcgcctgt cgatcctctg ggagctgcag cagatgcgta gcgaaggcct
     6541 gtttgccagc agtggattgg actggctagt tgaagggccg aagctgtcca gcgaacacgg
     6601 tcatctgggt tatacccgca tcaatgaaca ggcctatccc tacaaacagg actttatgca
     6661 gcagctggat caggccctgc tggcccttga acaggatggc agcatcaccc gcctggccca
     6721 acgctacagc tcgattgacc tgctacccgc caccggcaaa ggcgctgacg aggacgcaac
     6781 aagcggtaac gccccctgac caacatcaat tgccgaaagc agtgcacgcc cgtacactgc
     6841 gcgccttttt cctatgacgg gcgtaccaaa tgctttttct tctgcgcatg ctggcgatgg
     6901 gcgtgcactt tatcctcgcc ggcattctgg gcctgttgct cggcctgtgc cgcccgttca
     6961 atccagataa cagccgcctg tgcgcacgcc tctacagcat tccggcgcag tggctgctgc
     7021 gctggaagct gaagaccgat gtgcacagcc ttgagcacca gcactcctgc gtgatcgtcg
     7081 ccaaccacca gtccaactat gacctctacg tgctcggccg cgtagtgccc aagcgcacgg
     7141 taagcatcgg caagaagagc ctgaaatggg tgcccttctt cggccagctg tactggctgg
     7201 ccggcaatgt gctgctcgac cgtggcaacg cgattcgcgc caagcaggcc atgctcacca
     7261 ccaccgatac gctcaagcac aaggacacct cgatctgggt gttcgccgaa ggcacgcgca
     7321 acctcggcaa aggtctgttg ccgttcaaga aaggcgcgtt ccagatggcg attgccgccg
     7381 gtgtgccgat catcccggtg tgcgtcagca gctacgtcaa ccacctccag ctcaaccgct
     7441 ggaacagcgg cgagatcatg atccgctcgc tgccggccat ccccaccgcc ggtctgagcc
     7501 aggatgacct gccgcagctg ctcgaaacct gccgcaccca gatgcaacag tgcatcgatg
     7561 ccatggatca acagctcagc caaagcgcct gacgctaccg gtaatgcccc aaaagcccag
     7621 ccacgcgctg ggcttttatt gcctgatgta tcccgcgcag caaccgttca atcacaccag
     7681 cagcgagcaa gcagctaagc tgcgtgctga ggtcaccgat aataaggaaa cactatgggt
     7741 cgcgttgttg ccgccgccgt ttacagcaac ggcaagaaag tcaccaatat cagcctcgac
     7801 gaaggcaagc catggtcgaa gaaaccgggg catttcgtct ggatcggcct gcatgacccc
     7861 tgcgccaatg agctgatcag cttgcagcag caattcgacc tgcatgaact ggcgctggaa
     7921 gatgccctgc agcgacacac ccggcccaag ctggaaacct tcggcgatgc actgtttctg
     7981 gtgctctact cacccattct ggtcaacgac gagctgacct ttgtcgagac ccagctattc
     8041 gccggcagcg gttacatcat cagcgcacgc tacggcgaat ccgcgcccta ttcccaggtc
     8101 cgccaacgct gcgaggcgcg cccgttgctg cttgagcacg gcgaagattt cgtgctctac
     8161 gccctgctca gcttcgtgat cgagaactac cgcccgttga tggacaacta ccacgtcgag
     8221 cttgaggagg tggaacagca cgtgctgaaa aacccactga gccaggccga tgtcgagcgc
     8281 atccagggtt tgcgccgcga cctgctgcgc ttgcgccgct atatcgggcc gctggcggaa
     8341 atctgcaaag agctgcagcg tctcgacttc ccatttattg acaagaacat gcggccctac
     8401 ttccgcgacg tggcgatcca cgtcaaccgc ctgttggaag acctcaccgg cctgcgcgag
     8461 atggccgacc atgccatcga gatcggcctg ctgctggagt cctcacggca aagtgtcacc
     8521 cagcgcaagt tcgccgcctg ggcagcgatt ctggcctttc ccacggcggt ggcggggatt
     8581 tacgggatga actttcagaa catgcccgag ctcgcctggc agtacggcta cttcggtgtg
     8641 ctcggcttga tcaccaccgg ctgcgtcggg ctttacgtca gcttcaagcg atccggctgg
     8701 ctgtaaaagc caggtctgag ttggctgttg ttggcatgct caacccttgt aggagcgggc
     8761 ttgcccgcga atcggagtca ataggagaaa catcgcggcc aatgactcgg cgtccccgcc
     8821 gctcctacgg gtttcgcaaa gcccgccagc actgctgcaa aacttaacta ggacatagcc
     8881 ataaaaaagc ccggtcactg accgggcttt ttcgtgcagc cgtggattac tcggccaagc
     8941 cggcacggcc ctgagcgaca aagcgcatca tccactcggc caccgtgtga ccctgatggt
     9001 cgtgctgcag gctgtcgata cccttggagt aggcaggctc gccgatatgc tgttgacgca
     9061 ggtcgagcaa cgcgcgcgag tagtcatgca cgaactcagg gtgaccctgg aagcacagca
     9121 cctgatcgcc gatgcaatag gaggcaatcg ggcagaactc actgctggcc agcaatgtgg
     9181 cgtttagcgg caattgggtg acctgatcct ggtggctgat cagcagggtc aggtcttcca
     9241 gatccgggct catccaggtc ggcttgcctg ccatgcggta ttgatggata cccacacccc
     9301 agccctgcac ggcgcgttcg gccttgccgc ccagcagcag cgccagcaac tgatggccga
     9361 aacacacgcc gagcagtttg tcgccacggt tgtagcgctc cagcaagtag gttttcaggg
     9421 tctgaatcca ggcgtcgctt ccaaacgaat ccgccttgct gcccgtcacc agataggcat
     9481 cgtagcgctc gctgtccggc gggtaatgcc cctccatcac gttgtacacc ttaaactcgg
     9541 cagcaatcgg ctgacgggcg aacaactgct cgaacatacg gccataactg tcgtattggt
     9601 cgaccaattc agggcgaaga acatcggttt ccaggatgca tatctgcagc ggcatatggg
     9661 cggttaccta gggcgcagga gggttgtttt accagcgcgc aagcgtgcct gctgacaggc
     9721 tgcaaggcaa tagctacggg gcaaacccga ccaattaccc gaggtccatc agcgccctcg
     9781 atagtcacca taagcaccat tcacttctgt cgatagcagc acgcgcagat gatctggcca
     9841 tcgggcactg cgccccactt accagaggac atcagcatga ccagtttttc cgccatcgcc
     9901 agcctgcttg ccgcatccgc ctttaccagc aaccacagcg cgagcagtca gcgccaagcc
     9961 gaaaaagtcc aagccgcaca actcaaggct gaccagaacg ccggtaaata acgtcacacc
    10021 gaaatcagga gaacgccatg agcatcgccc aatcgctgag caaccagaac gtttacggcg
    10081 tgacctacgc cactgtcgac gacagtggca tccacttcga atccgaactg gccatccagc
    10141 ttagcgatgg caccctgact accctgcgca tgcccactca tctgagcgag cggcaggcca
    10201 ttcaacagct ggtgtgcggc cgtcaggcct gctgacagtt attgcgctgg catcgggcag
    10261 ctaaggtcgc cttcttcaca gacggccagc tgctccagct ccctgacccg cgcgacggtc
    10321 ttttgcgtca gacagccgtt aagcaccatc ggataggccg aaccgccctc caccgaggcc
    10381 gactcgaacg cgcaggccag atcgcggaac ttgatccagg ccagctgcac ctccttgagc
    10441 tgttgcttct gcccatcatc cagacgcttg cggtaatcgc cgtacagctg attcaggcgt
    10501 ttgtcctgag cggcgtagcc cgccgccgcg cactggttga gggtgaactg atccatggca
    10561 ttggcgcagt catccgccca agacggagca gcccaaaagc cagccagcac taaagcgcac
    10621 actttcagaa tctgcataca acgctccttg atcgtataaa cctgattgtt acttgaacac
    10681 gctactgagc agccaacagc ttctggttgg ccttggcgca gtacgcttgc tgacgctgag
    10741 cctgcggcat actttgcagg gtgctcgtgc actgcgccaa gctagttgtt tgacgtaggt
    10801 gcatgcgggc agtcgcgtca acttctacca ggtaggcgta gtcgtaccta gccttgtcat
    10861 accaggagtc aataggttgc tcgctgacca cacagcgata ctgtaactgc acggtatagc
    10921 ccttcatgcg gtggctcata aagatatggt gataacgcgc ccaatcacgc tcacgtatgc
    10981 ccttcaatcg agcctcttcg cagacctcaa gctgagtaat ttccggctca tggagaaaca
    11041 ccacctgtgc caggttcgag cccccgagat tagctagagc caccagatac acccgctcat
    11101 cggcagcctg cgcagtgccg agcaatgcca tcagcagcac gccgattagt ccctgtttca
    11161 tgtttagctc cccaaaaaac cacagcctaa acaagccaac ggcaactggc catcaggcgc
    11221 aacaaaaaag ccagctcaca gagctggctt cgatgtgcca cagaaggtgc ttaaaacagc
    11281 tcgttcttcg cggctttttc cagcagcagc gctggtggca ggaaacgctc gccgtactgc
    11341 tcagccaaat actgggcgcg agcgacaaag tccttcaccc cgtactggtt gataaactgc
    11401 agcgcaccgc cgctccaggc ggcaaagccg atgcccatga tcgagccgat attggcgtcg
    11461 gcaaccgact tgagcacacc ctcttccacg cagcgcacgg tttcgatggc ctggataaac
    11521 aggatgcgat cacgtacgtc gatctgcgag atctgcgcgt ccggcttctc gaagcgtgcc
    11581 ttgagctccg gccacaggtg cttcttgccg cccgccgggt aatcgtagaa gcccgcaccc
    11641 gccaccttgc ccggacgctt gtactcgccc agcatcatat cgatcactgc gaacgccggg
    11701 tgctcgggga tctgcttacc ttccgcagca aggtccttga tggtttgctg acggatatgg
    11761 ttcatcaggc tcatcgacac ttcatcgctg atcgccagtg ggccgaccgg catgccggcc
    11821 ttacgcgctt cgttctcgat catcgccgcg ctcacgcctt cgcccagcat ggccagacct
    11881 tcgttggtaa aggtgccgaa cacccgcgag gtgaagaagc cgcgactgtc gttgaccacg
    11941 atcggagtct tcttgatctg catgacgtag tcaaagccac gcgccagggt ctcgtcgctg
    12001 gtgtgctcgc ccttgatgat ttccaccagc ggcattttgt ccaccgggct gaagaaatgc
    12061 aggccgataa acttgtcctg cttctgcacc gcgttggcca ggccagtgat cggcagggtc
    12121 gaggtgttgg aggctattac agcatcactc agcgccgcac gctcagcagc cgcagtgaca
    12181 ttggctttaa ggtcacgatc ctcgaatacc gcttcgataa tcaggtcgca gccttcaaag
    12241 tcggcatcgc tgacggtggg tttgattcgc gccaggatgc catcgcgctt gtcggcgctc
    12301 atctggccac ggccgacttt cttttccagc agcttggccg agtaatcctt gcccttctcg
    12361 gccgcctcga tggagatgtc cttgagcacc acctcaatcc cagccaccgc cgacacatag
    12421 gcaataccgg cgcccatcat gccggcaccg agcacgccaa ccttcttggt cacgtagggt
    12481 tcgaagcctt gcgggcgcga gccgccagcg ttgatttcat tgagttggaa ccagaaggtg
    12541 ccaatcatgt tcttcgccac ctggccggtg gtcagctcgg tgaagtagcg tgcctcgatg
    12601 atctgcgcgg tgtcgaagtc gacctgggca ccttcgaccg cggcgcacat gatcttctcc
    12661 ggcgcgggga aacatccctt ggttttgtcg cgcagcacgc tcggggcgat ggccagcatc
    12721 tgcgccacat tcgggctgga cggcgtaccg cctgggatct tgtaaccggc gacatcccaa
    12781 ggctgcttgg gcgccgggtt ggccgcgatc caggcgcggg ccctggccag cagatcatcc
    12841 ttgtcggaag ctaattcgtg gatcaagcca gccttgagcg cggcatcggg acggaccttc
    12901 ttgccttcag ccagatacgg cagggctttt tccaggccga gcaggcgcac catgcgcacc
    12961 accccgccac cgccaggcag caggccgaga gttacttccg gcaagccgag ctggatgctg
    13021 gaactgtcca gggcaatacg gtgatggcag gccagggcaa tttcccagcc gccgcccaaa
    13081 gccgcaccat tgatggcggc gaccacaggc ttgcccagac attccaggcg gcgcagttgg
    13141 cccttgatgt tgaggatcat ctggtagaag gccggcgcgt ctgccttggt gaccttgatc
    13201 agctcgttca gatcaccgcc ggcgaagaag gtcttcttcg cggaggtgac gatcaccccg
    13261 gcaatggagt ccttctcggc ttccaggcgc tcgacgattt tgcccatggc ttcgcggtac
    13321 accgcgttca tggtgttggc gctctggcca ggcatgtcca tggtcagaac gacgatattg
    13381 tcctggcctt tttcgtaacg gatggcgtca gtcatttcta ttcacttccc aaatgtcggg
    13441 ggagcgcttc aggggcgtca cgagcgaagg tcaggcaagg cggaaatcat tcggaaagcg
    13501 gagtttgctg gtgcaaatga gcatttccgg aggatttcca acgcagcatg accgagcgca
    13561 gtagcccgtg atagctcctt aaatacgttc gataatggtg gcaataccca taccgccgcc
    13621 tacgcagagc gtggccaggc cataacgcag cttgcgcttt tccagctcgt cgagcagggt
    13681 gccgaggatg gcgcaaccgg tcgcgcccag cgggtggccc atggcgatgg aaccgccgtt
    13741 gacgttgacc ttgtcctcgc tgacgcccat gtccttcata aatttcatca ccaccgaggc
    13801 aaaggcctcg ttgacctcga acaggtcgat gtcattgacg ttcagcccgg ccttggccag
    13861 tgccttgcgc gtggccggcg cagggccggt gagcatgatg gtcgggtcgg tactggtcac
    13921 ggcggtagca acgatgcgcg cgcgcggctt caaacccagc tccttaccct tggcctcgga
    13981 accgatcagc atcaaggccg aaccgtcaac gatgccggag ctgttgcccg gggtgtgtac
    14041 gtgtttgatc tgctcgacat ggctgtaaac gcgcagcgcg gtgctgtcga agcccatctg
    14101 gcccatcatc tcgaagctcg gcttgagttt gcccagacct tctagggtgg attcgccacg
    14161 gataaactca tcgtggtcga gcagcacgat gccgttctgg tccgccaccg ggatcagcga
    14221 cttgttgaac gatccgtcct tgctggcgcg ggcggctttc tgctgcgagc gcagggcgaa
    14281 gctgtcgacg tcagtgcggc taaagccttc cagggtagcg atcaaatcag ctccgatccc
    14341 ctgtggggtg aaatgggtgt gcatattggt cgctgggtcc atcacccagg cgccgccgtc
    14401 ggaacccatg ggaatgcgcg acatcgactc cacgccgccg accaccacca gatcctcgaa
    14461 accggagcgc accttcatcg cgccgaggtt caccgcttcc aggcccgagg cgcagaaccg
    14521 attgagctgc acgcccgaca cgctgacatc ccaatcggca accatggcgg cggtcttggc
    14581 gatgtccgcg ccctgatcac ccaccggcgt tacgcagccg agcacgatgt catcgacctg
    14641 gctggtgtcc aggttattgc gcgcttgcag cgccttgagc aggccggcga tcaggtcgac
    14701 aggcttgacg ctgtacagcg cgccgtcctt cttgcctttg ccgcggggcg tgcgcactgc
    14761 atcgaaaata aatgcttcgg tcatgacgtc ctcagacctc caatggaggg tggctgacgg
    14821 cgtaggtcga gcccacgcct gtttttgttg agtgcagtca ccatacgccg cgcgcagaca
    14881 ggctcaatga ccgaagtgct caggcctatt gaccgctcag ctcagacaaa cggtcaatca
    14941 ctgggcgaag gccagcaagc gcgtctagct caaggcccac ttacctatta cgagccccgc
    15001 gtcataaccg ctggcaacta acccgcgcat cttagtaatc aaggcgtcta tagcccgccg
    15061 ctgcgcgccc ctacagttga ttcgagccac ctgcaaatag tgcgcagatc ggctgctgta
    15121 ggcgtcgtcg ccgggttttg ccggggcggc ttgaataatc aacacaaggc gtggcgtcgc
    15181 atccgcatac agagcaggac agcgacgacc agccgcaaaa aacaacaagg ccgaccgcca
    15241 tgttcaactc agtaaaaatg cgtcaggcag gcatgatcgt atttgccacc acagtcattc
    15301 tgattcttcc caacctgacc cgcctggtca gctgaaaacc tgatttcctg ccctacctgc
    15361 ccccctggag tgagcaggca gcggcagagc agcctctaca aacgcgtgca aagcagcgcc
    15421 tgtgctaggg tgatttcaat caacccaagg agcacgccat gcgcacccta cttgccaccc
    15481 taagcttgct gatcagcctg cctctgctgg caggcgaagc cgaactcagc gccgccgttg
    15541 tcgccctgca atcaccggaa agcgtactaa tcgatgtgcg caccgccgat gaattcgccg
    15601 ccggcgcctt gcccggtgct gagcagattg agcatgaaca gatcgccagc cgcatcaacg
    15661 ccatcgcccc ggacaaggac acgccgatca tcctctactg ccgcagcggc cgccgttccg
    15721 gcatcgccga agaaaacctg cgcgccatgg gctacagcaa cctgatcaat gctggcggct
    15781 atgacgaact gaaactcgcc ctggagtcgc aggattgaac aagcgcaccc tctgcatcgc
    15841 cctgctcgcc ctcttaccct tcagcaacct gcacgccgcc gaactgaccc tggaactggg
    15901 ccacggcagc caacgcctga gcagcgccca actgctcgcc cacccacagg cgcaaaacgt
    15961 cgagatcagc gacgacgtca gctacaaacg cagcatgcgc taccgcgccg tgccgttggc
    16021 cgcgctgctg gaaggtgtag agccgggtga tcacctgcaa ttggtagcca gcgacggctt
    16081 tgccgccgaa ctctccgccg cccccgcgct cagccaaacc ggcagccagg cctggctggc
    16141 catcgaagac ccagcgcacc cctggcccgc cctgggcaac ggcaaaccca gcgccggccc
    16201 gttctacctg gtgtggcaaa acccagccgc cagccagatc ggccccgagc aatggccatt
    16261 ccagctggcc accatccgca aactggcccc ggtggcgcag cgcttcccgc aattactgcc
    16321 agccgcagac gccagcccgg cggtgcaggc cggttttgcc caataccaga aaaactgcat
    16381 ggcctgtcat cgcctgaatg gcgcaggcga ctcgcaattc ggcccggacc tgaatattcc
    16441 gcataacccg acggagtatt tcagtggcga ctttctaacc ctctatatcc gcgacccgca
    16501 gagcttgcgg cgctggccgc agggcaagat gcctgggttt gcggttgagg tgttgagtga
    16561 caaggaattg ggggagttgg ttgagtattt ggggcatatg gtggggcgca aagtgcagcc
    16621 tttgtagatt gatggcagaa ccgtaggatg ggtcgggccg cgcaggttga gcaacgcgag
    16681 ttttagcttt gcttttggtt cagccacaca aaacgtacaa acgacgccaa acgccccttc
    16741 aggagggtga acagagctgt tgtgtagagg ggtgagccgc atggatgcgg cgaaaggcgt
    16801 aaagggccag ggacggccct tgtacgccga ccctcggagc aatgattctg ttcacgaacc
    16861 ctgccgaagg cagggccgga tgtcggggct agacctttaa gttccttttg gggcgactgg
    16921 cacagccgaa ggctgaacgt gctttagcac ggccccgaag gggtgagcga agcgaatcaa
    16981 agggactcgc ccgtaagggg cgaaaccaaa actagagcct gcaaccgctc ggtgggttac
    17041 gtggcgctca agcttcgaat tgcacaatca acctcagatc agcgccacta acccacccta
    17101 cgaagccagc gcagcgcccc tactgctgtt gcacccgcac cagcggtgca acctccacct
    17161 gcgcatgcat ctgttcaatg ccgccacccc ggcgcatgcc gcgcaccggg caggcatcca
    17221 gatagtccag gcccacggct aacttcaaat gccgctccgg cttggccagg cagttggtca
    17281 cgtcaaagct gtaccaggcg tcatccagcc aggcttcagc ccaggcgtgg ctggccaggt
    17341 ggtcttcgct gtcggtaaac agatagccag acacatagcg cgctggaacg cccaggctgc
    17401 gcgcgcaggc gagaaaggcg tgcgcgtgat cctggcaaac gcccttgccg ccggcgaagg
    17461 cttcactggc gctggtttcc acggcggtgc tgcccggctg atagcggata tgtgcattca
    17521 acgcatgcat cagatcgatc aagccgctgc ggtccggccg gctgcggctc tgcagcttag
    17581 cgaattcacg cagggcttcg tcggcctcgg tcaggcgggt aaagcgcaga aacggcagcg
    17641 ccgactggct ttcgtgctcg gcctcgcgcg cttcgtcgat ttccacctgg ccacgggcgc
    17701 cgatcacgat ggattcgtgc ggctcgtcca tggtcagtac atgcaggata ttgccgtaag
    17761 ggtcgagctg cgcacgcacc ggacgcggca gggtcagctg ccagctgagg acctgctggc
    17821 gctcgctgtc gtgcggagtc atgcgcaggt actggatgct ggtgcgcacc tggtcttcat
    17881 agtgataggt ggtgtcgtgg ctgattgaca gtctcatgcg acctccaggt aggaactttg
    17941 gatggcttgg cctagctggc gaaccagcag gatgaaatcg gtgagccagg cgtgcaggcc
    18001 ctcgtcgagt acttcttcga tgctggtgta acgcagccgt gcttccagtt cagcggccag
    18061 acgctgagcc gggcgaccgt tgtcgccggg caggctggcg agcatcaagc tcagttcgtc
    18121 catgcaagca cgcagcgagc gcggtacatc ggcacgcaat agcagcagtt cggagacttt
    18181 gcgcgtaccg ggcgagccgc ggtagatctc agcaaaggcc tcgaacgagg acagtgcgcg
    18241 caaaagcgcg gtccactggt agtagctgcg cgccgggcgg ccgtcgattt catcgatgtc
    18301 ttcgccaagc atttcatagc gcgcatcgag caggcgcagg gtgttgtcgg cgcgctcgat
    18361 aaaggtgccg aggcggataa agcggtacgg gtcgccgcgc atgatggtgc cgaaggtggc
    18421 gccacgaaac aggtgcgagc gctccttgac ccactcgcag aaacggctga tgccatagcg
    18481 actcaggcct tgctgggcga taacgtggat ttccagccag gtggcgttga tgttttccca
    18541 catgtcggcg gtgatccgcc cgcgcacggc gtgggcattg ctgcgcgcgg cctgcaggca
    18601 gcaatagatg ctcgccggat tgctcgcatc aagggcgaag aagtgcagca tgcgctcggc
    18661 gtgcagcgga ccgtggcgct ccaggtaatc atccagggtg ccggtaatca gcagcggcag
    18721 ggccagttcc tccaggccat caccacgacc gtcctgcggc atcagcgaca gcgaatagct
    18781 gacttcaagc atgcgcgcga ggttttccgc acgctccagg tagcgcgaca tccagtacag
    18841 atcagaggca gtacgactca gcatgatgaa tcctccacca cccaggtgtc tttggtgccg
    18901 ccaccctggg acgagttgac caccagcgaa ccttcgcgca gcgcgacgcg ggtaaggccg
    18961 ccggggacga tgcgggtttc cttgccggac aggacaaacg gccgcaggtc gatatgccgt
    19021 ggcgcgatgc cacgttcaac aaaagtcggg caggtcgaca ggcatagggt cggttgggcg
    19081 atataggcct cggggcgggc aatcaggcgg gcacggaagt cttcgatctc ggccttagtg
    19141 gcggccgggc cgaccagcat gccgtaaccg ccggagccct gagtttcctt gaccaccaag
    19201 tgttccaggt tggccagcac gtgggacagc tcagccggct tgcggcactg gaaggtcggc
    19261 acgttcttca ggattggctc ttcgctgagg tagaagcgga tcatctcgtc gacatagggg
    19321 tagatggatt tgtcgtccgc caccccggtg ccgatggcat tggccagtac cacgttgccg
    19381 ctgcggtagc aggccagcag gccaggtacg ccgagcatgg agtccgggtt gaaggccagc
    19441 ggatcgagga aggcgtcgtc aaggcggcga taaaccacgt ccacctgctt ggccccggca
    19501 gtggtgcgca taaagagttt gtcgtcacgc acaaacagat ccgcgccttc gaccagctcc
    19561 acgcccattt cacgggaaag gaatgcgtgc tcgaaatagg cgctgttgaa acgccccggt
    19621 gtcagcacca ccacgttggg gttatccagc gggctggagg acttcagcgt atcgagcagc
    19681 agattcgggt agtgatcgat cggcgccacg cgctgggcgg cgaacagctc ggggaacagg
    19741 cgcatcatca tcttgcggtc ttcgagcatg tagctgacgc cgctgggggt gcgcaagttg
    19801 tcttccagca cgtagtagct gccgtcacca tcgcgcacca ggtcgacccc ggcgatatgg
    19861 gcgtagaggt cgcggtgcag gttgaggccc tgcatggcga tctggtaacc ctcgttggcc
    19921 agcacttgct cggcggggat gatgccggcc ttaatgatgc gctggtcgtg atagagatcg
    19981 gcgaggaaca tgttcagcgc ttgcacacgc tggctgcagc cgcgctcgac gatgcgccat
    20041 tcgctggcgg gaatgctgcg cgggatgata tcgaacggga tcagccgctc ggtgccctgg
    20101 tcgtcgccgt acagggtgaa agtgataccg gcgcggtgaa acagcagatc ggcttcacga
    20161 cggcgttggg ccaacagttc atccggcgta tccgctaacc agcgggcaaa agcttggtag
    20221 tgcgggcgga cagcgccact cgcgtcgtac atctcatcat aaaaggtgcg ggccatgccg
    20281 tactccttgt ctcccggaca tgcagacatg ctttgcaagg ggcgagccat acaacaaaag
    20341 tgttttaaat cagtaacttg agcatttacg atgcgagcaa cgcaccaagc cagtgcgcaa
    20401 tcgtgaccgc cgagtcgcag ggcgcgccat tttaaggcgc gtgttggctg tggtttgcaa
    20461 ccgcatacgt aagcacggca gggaatgcgc gtatcagagc ccggccagct gcaaccagtg
    20521 acggtaaaag ccttcggcga cgcggttcag ttcggcaacc ttggcgggta gctcttcgag
    20581 catctgcgcc ctgctctgct gactaggctg ggtttcatcc aacaggtgat gccagtcgcc
    20641 gagccattgg cgcaccaagg cttcgtccat ttccgggtgg ccttgaagcg ccagcacttt
    20701 atcgccccag gcaaacgcct ggttgctgca ccaggggctg ctcagcaagg gctgcgcacc
    20761 cttgggaatg gcgaagctgt cgccatgcca ctggaagata gggaactgct ccggcaggtg
    20821 ctgcagccag gggctgggcg ccgcctgatc aagacggctt agcggctgcc agccactctc
    20881 ggtgtagggc atgcggctga tttccgcgcc cagagccttg gccaacaact ggccgcccag
    20941 gcaatggccg atcagcggga tatcgcggcg gataaaatgc tgcagcgcgg ccacttcggt
    21001 ctgcagccag ggcagcgggt cgttgacgct catcggcccg cccatgatgg ccacggcctt
    21061 gggccggtcg aggtcgatgc catccagctc gccaagatcc gcgcgcatca cggtaaatga
    21121 gtggcccgcc tgctccagca ccttgcccag gtgggccggc gggcagtaat cgatgtgggt
    21181 aaaaatcagg atatcggtca tgcgccaagt ctgaagcaaa gcgcaggctg gggtcgaggg
    21241 acgattgatt ggggatagtt tgcaggggtt gaaatgacac tggccagctt atggctggcc
    21301 agtgtgcaga cttcagggct gtctcacctc ctgggttacg ccccacccat ccagtttgcc
    21361 gccaagcggc gccacgaccg actccaggtc atgctcgaaa tcatcgatgc cgtggtatgt
    21421 cgcgaacatt actttactga cttgcaggtg ccagacaccg tcatcacgct cggtcaccag
    21481 ggcgtgtaaa gactcacctc gaaatttgcg ggtggccatg cgggcccgct cctcatccgg
    21541 gaataaggcg tagaactcga tgggatggaa gctggcaaag tcgaagccac cctccttcat
    21601 ccgccgtaaa accccgttac tgacatcatc gtgcaaggct gtgctcatga agtgacctcc
    21661 tcaggcgatg gacaaattta caggacg
//
LOCUS       DASRUM010000017         5744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1013637, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000017 DASRUM010000000
VERSION     DASRUM010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013637"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1489
                     /locus_tag="VFF22_01255"
     CDS             23..1489
                     /locus_tag="VFF22_01255"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019472117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZX15481.1"
                     /translation="MWKVLPVTQKPDQCLGEWIDREALAEAMIPMIGQLYRNNNVVTS
                     IYGRGLINRSVIDILKAHRFARHRLTGDSELSVHDTFLVLKAMSELKLGAASVDLGKM
                     VSKFKAEGQGRSVEQFVKDELIEVAGKQNSEAREGTDVVLYGFGRIGRLLARILIEKT
                     GGGDGLRLRAIVVRKGATNDLVKRASLLRRDSVHGKFDGTITIDEENNTLTANGNLIQ
                     VIYAKSPAEVDYTQYGIKNALLVDNTGVWRDADGLGQHLACPGVDRVVLTAPGKGALK
                     NIVHGINHGEITPEDKIISAASCTTNAIVPVLKAINDQYGILNGHVETVHSYTNDQNL
                     IDNFHKGDRRGRSAPLNMVITETGAATAAAKALPVLKGKLTGNAIRVPTPNVSMAILN
                     LNLEKATTREEVNEYLRQMAMHSDLQKQIDFVQSQEVVSTDFVGSRHAGVVDAEATIC
                     NDNRVVLYVWYDNEFGYSCQVVRVMEDMAGVNPPAFPR"
     gene            complement(1556..2116)
                     /locus_tag="VFF22_01260"
     CDS             complement(1556..2116)
                     /locus_tag="VFF22_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003172583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HZX15482.1"
                     /translation="MRLLLALTTLLLLSGELFANNRERLQEANFAATHQFEQNSLERK
                     NQSVLTYLWADVYAAAFYAPAQASARQAVTKPLTQRLELYYFRDIDSQDVIKAAWVTL
                     ERQHDTATLQRLRPEIDALHATFRDIRPGDRYALNFNANSGLTLEVNGKVTYTSQDPA
                     LAKAYLGIWLSPNGLSDKLRTSLLAD"
     gene            2387..3724
                     /locus_tag="VFF22_01265"
     CDS             2387..3724
                     /locus_tag="VFF22_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HZX15483.1"
                     /translation="MIKIKHGLDLPITGVPVQRIEAARPVRSVAVIGFDYHGMKPSME
                     VQVGDRVKLGQVLFSDKKSPGVQYTAPAAGVISAIHRGEKRVLQSVVIDVEGEEQITF
                     DQVTVDQLATVGDAKVREVLLQSGLWTAFRARPFSKVPAADAQPSSIFVTAIDTHPLA
                     ADPAVVIAEYAADFTNGLQVLSNLAKVFLCKAEGASLPGEQLSKVHTEAFAGPHPAGL
                     PGTHIHFLDPVSVSKSVWSINYQDVIAIGKLFTTGQLWTERVVALAGPVVEQPRLVRT
                     RLGASLSELTAGQLQPGFNRVISGSVLGGRTAQGAFAFLGRYHLQVSCLVEGHERELM
                     HYLRAGVNKHSVMNIFVSKLNAAKKFAFSTTTNGSPRAMVPVGNYEAVMPLDILPTQL
                     LRYLIVGDTEMAQKLGCLELDEEDLALCTYVCAGKYEYGPILRDNLARIEKEG"
     gene            3728..4939
                     /locus_tag="VFF22_01270"
     CDS             3728..4939
                     /locus_tag="VFF22_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HZX15484.1"
                     /translation="MGIRAFLDKVEHNFEKGGKYEKWYALYEAVDTFFYRPGSVTKTT
                     AHVRDGIDLKRMMITVWLCTFPMMFFGMWNTGYQANLIFANSPELLTAQEGWRFALIG
                     ALAGFDPNSLWDNFIQGAAYFLPVYATAFIVGGFWEVLFASIRKHEVNEGFFVTSVLF
                     ALILPPSIPLWQVALGISFGVVIGKEVFGGTGKNFLNPALTGRAFLFFAYPAQMSGDA
                     VWTAVDGFAGATSLSLAAAGGIENVVSNGITWMDAFVGTIHGSMGETSTLAILIGGGI
                     LLITKIASWRIVSGVMLGMIGFSLLLNMLGSSTNPMFAMPWHWHLVVGGFAFGMFFMA
                     TDPVSASMTNTGKWIFGALIGVMVVMIRVVNPAFPEGMMLAILFANLFAPLIDHFVVQ
                     ANIKRRLARNV"
     gene            4932..5720
                     /locus_tag="VFF22_01275"
     CDS             4932..5720
                     /locus_tag="VFF22_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HZX15485.1"
                     /translation="MSSQKESTVRTLVVALLVCLVCSVFVAGAAIALKPTQVENRQLD
                     KQRSILAIAGLGDASMSSAQVKALFAERISARIVDLDTGTFSDAQNPATFDPLKASKD
                     PKLSAVLEGSDDIASIKRRERFTTVYLVEQDGKLETVILPVRGYGLWSTLHGFMALQG
                     DLNTVAGFGFYQHAETPGLGGEVDNPKWKSLWLGKTLFDDKGELAIQIIKGNVDAQSA
                     QATHQVDGLAGATLTSNGVNNLLHFWLGENGFAQFLANLQKGEA"
BASE COUNT         1121 a         1609 c         1744 g         1270 t
ORIGIN      
        1 agcccctttt ttcagcccct acatgtggaa ggtactgccc gtgactcaga agcccgacca
       61 gtgtctcggt gagtggatcg atcgagaagc ccttgcggaa gcgatgattc cgatgattgg
      121 ccaactctac cgtaacaaca acgtggtcac ctcgatctac ggccgtggtt tgatcaaccg
      181 ctctgtgatc gacattctca aggcccaccg cttcgcacgt caccgcctga ccggcgacag
      241 cgaactgtcg gtgcacgata ccttcctggt gctcaaggcc atgagcgagc tcaaactggg
      301 cgccgcctcg gtggacctgg gcaagatggt cagcaagttc aaggccgaag gccagggccg
      361 cagcgttgag cagttcgtta aagacgagct gattgaagtg gctggcaagc agaacagcga
      421 agcccgcgaa ggcaccgacg tggtgctcta cggcttcggt cgtatcggcc gtctgctggc
      481 gcgcatcctg atcgagaaaa ccggtggtgg cgacggcctg cgtctgcgcg ccattgttgt
      541 gcgcaaaggc gcgaccaatg acctggtcaa gcgcgccagc ctgctgcgcc gcgactcggt
      601 acacggcaag ttcgatggca ccatcaccat cgacgaagaa aacaacacct tgaccgccaa
      661 cggcaacctg atccaggtga tctacgccaa gtcgccggcc gaagttgatt acacccagta
      721 cggcatcaag aacgccctgc tggtggacaa cactggtgta tggcgtgacg ccgacggcct
      781 gggccagcat ctggcctgcc caggtgtcga tcgcgtagtc ctcaccgcac ctggtaaggg
      841 cgcgctgaag aacatcgtgc acggcatcaa ccacggcgaa atcacccctg aagataagat
      901 catctcggcc gcttcctgca ccaccaacgc catcgtgccg gtgcttaaag cgatcaatga
      961 tcagtacggc atccttaatg gtcacgtcga aaccgttcac tcgtacacca acgaccagaa
     1021 cctgatcgac aacttccata agggcgatcg ccgtggtcgc agcgcaccgc tgaacatggt
     1081 gatcaccgaa actggtgctg ccactgctgc tgccaaagct ctgccagtgc tgaaaggcaa
     1141 gctgaccggc aacgccattc gcgtaccgac gccgaacgtg tcgatggcca ttctcaatct
     1201 caaccttgag aaagctacca cccgtgaaga agtcaacgag tacctgcgcc agatggccat
     1261 gcactcggac ctgcagaagc agatcgactt cgttcagtcg caggaagtgg tttccactga
     1321 cttcgttggc tcgcgtcacg ccggtgtagt cgatgccgaa gccactatct gcaatgacaa
     1381 tcgcgttgtg ctgtacgtgt ggtacgacaa cgagttcggt tacagctgcc aggtggttcg
     1441 tgtgatggaa gacatggccg gggttaaccc gccagctttc ccgcgctaag cgcaggtaac
     1501 gccataaaaa acgggagctt cggctcccgt ttttttatgt ctgggattgc tgggattagt
     1561 cggccagcag gctggtgcgc aacttatccg acaggccgtt gggtgagagc cagatgccca
     1621 ggtaggcctt ggccagggct gggtcctggc tggtgtaggt gactttgcca ttcacttcaa
     1681 gggtcaggcc gctatttgcg ttgaagttca gggcgtagcg atcgccgggg cgaatatccc
     1741 ggaaggtggc gtgcagagcg tctatctccg gacgcagacg ctgcagtgtg gcggtgtcgt
     1801 gctggcgttc gagggtgacc caggctgcct tgatcacgtc ttggctgtcg atgtcgcgaa
     1861 agtagtagag ctccaggcgt tgcgtgagtg gcttggtgac cgcctggcgg gcactggctt
     1921 gggcgggggc gtagaaagct gcggcgtaga catcggccca caggtaggtc agcacgctct
     1981 ggtttttgcg ttccaggctg ttctgctcga actggtgggt ggcagcgaaa ttggcttctt
     2041 gcaggcgttc gcggttgttg gcgaataact cgccgctgag cagcagtagg gtggtgagcg
     2101 cgagtagcag gcgcatggca aggctccagt tagtctattg gtcttatgtc gcgcgccggg
     2161 cctctgcagt caagccgcca tctgttgcct tctgttacgt taaaccggat aaaagcaagc
     2221 gcttgggtgg ccagtggccg aaggtgcgac ttataatcgg ccagattttt tcccgcgacc
     2281 gacatcctgc ccagcgccca atctgttttg gcgttgcgcc gcatttttct gcgttggtgt
     2341 cgtccgtatt ctaaaaaagt ttataaatca gtggttaggc aaacctatga tcaaaataaa
     2401 acatgggctt gatttgccca taacaggcgt gccggtgcag cgcattgagg ctgcaaggcc
     2461 ggtgcgtagc gtcgccgtca taggcttcga ttatcacgga atgaagcctt cgatggaggt
     2521 tcaggtcggt gaccgggtca agcttggcca ggtgctgttc tcggacaaga agtcgccagg
     2581 cgtgcaatac accgctccag ctgcaggcgt gatcagcgcc atccatcgcg gcgagaagcg
     2641 agtgctgcag tcggtggtga tcgatgtcga gggtgaagag caaattacct ttgatcaggt
     2701 aacggtggat cagctggcga ctgtcggcga cgccaaagtg cgtgaagtgc tgctgcaatc
     2761 gggcctgtgg accgcatttc gcgcccgtcc gttcagcaag gtgcctgctg cagatgcgca
     2821 gcccagctcg attttcgtca cggccattga tacccacccg cttgcagccg acccggctgt
     2881 agtgattgcc gagtacgccg cagactttac taacggtctg caggtgctga gcaatctggc
     2941 caaggtgttt ctgtgcaagg ctgagggtgc cagcttgcct ggcgagcagc tgagcaaggt
     3001 gcacaccgaa gcttttgctg gtccgcaccc tgcgggcttg ccgggtacgc atattcactt
     3061 tcttgatccg gtcagcgtca gcaagagcgt ctggtcgatt aactatcagg acgtgatcgc
     3121 catcggcaaa ctgttcacca ccgggcagct gtggaccgag cgcgttgtcg cgctggccgg
     3181 ccctgtggtc gagcagccgc gcttggtgcg cacccgtctg ggcgcgagcc tcagcgagct
     3241 gactgccggc caactgcaac ctggtttcaa ccgggttatt tcaggctcgg tgctgggtgg
     3301 ccgtaccgcg cagggcgctt tcgccttcct cggtcgttat cacctgcagg tgtcctgcct
     3361 agttgaaggg catgagcgtg agctgatgca ttacctgcgt gccggtgtaa acaagcactc
     3421 ggtgatgaac atctttgttt ccaagctcaa tgctgcgaag aagttcgcct tctccaccac
     3481 caccaatggc agcccgcgcg ccatggtgcc ggtgggtaac tacgaagccg tgatgccgct
     3541 ggatatcctg ccgacgcaac tgctgcgcta cctgatcgtc ggtgacaccg agatggctca
     3601 gaaactgggc tgcctggaac tcgatgaaga agacctggcg ctgtgcacct atgtttgtgc
     3661 cggcaagtat gagtacggcc cgatcctgcg cgataacctc gcccgtattg agaaggaggg
     3721 ttaagtcatg ggaattcgtg cattcctcga taaagtcgag cacaacttcg aaaaaggcgg
     3781 caaatacgag aagtggtatg ccctctatga ggctgtcgac accttcttct atcgccctgg
     3841 cagcgtaacc aagaccaccg cccacgtgcg tgacggcatc gacctcaagc gcatgatgat
     3901 caccgtgtgg ttgtgtacct tcccgatgat gtttttcggt atgtggaaca ccggttatca
     3961 ggccaacctg atcttcgcta acagccctga gctgctgact gcccaggaag gctggcgctt
     4021 tgcgctgatc ggcgcgcttg ccgggtttga ccccaatagc ctgtgggata actttatcca
     4081 gggcgcggct tacttcctgc cagtttatgc cacggcgttt atcgtcggtg gcttctggga
     4141 agtgctgttt gcttcgatcc gcaagcacga agtcaacgaa ggtttcttcg tcacctccgt
     4201 gctgtttgcc ctgattctgc cgccgagcat tccgctgtgg caggtggctc tgggtatcag
     4261 cttcggcgtg gtgatcggca aggaagtgtt tggcggtacc ggcaaaaact tcctcaaccc
     4321 ggcgctgacc ggtcgtgcct tcctgttctt cgcctacccg gcgcagatgt ccggtgatgc
     4381 ggtctggact gcggttgacg gctttgccgg cgcaacctcg ctgagcctgg ctgctgcggg
     4441 tggcattgag aatgtggtga gcaatggcat cacctggatg gacgcctttg tcggcaccat
     4501 tcacggttcc atgggcgaaa ccagtaccct ggcgatcctg attggtggcg gcattctgct
     4561 gattaccaag attgcctcct ggcgcattgt ttccggcgtg atgttgggca tgatcggctt
     4621 cagcctgctg ctcaatatgc tcggttcgag caccaacccg atgtttgcca tgccttggca
     4681 ttggcacctg gtggtgggcg gttttgcttt cggtatgttc tttatggcca ccgacccggt
     4741 gtcggcgtcc atgaccaata ccggcaagtg gattttcggt gccctgatcg gggtgatggt
     4801 ggtgatgatc cgtgtggtca acccagcctt ccctgaaggc atgatgctgg cgattctgtt
     4861 cgccaacctg tttgcaccgc tgatcgacca ctttgtcgtt caagccaata tcaagcggag
     4921 gctggcacgc aatgtctagt caaaaagaat ccaccgtccg caccttggtg gtagccctgt
     4981 tggtctgcct ggtgtgctcg gtgtttgttg ccggtgcagc catcgcgctg aaaccgactc
     5041 aggtggaaaa ccgccagctg gacaagcagc gcagcatcct ggcaatcgcc ggtttgggcg
     5101 atgccagcat gagcagcgcg caggtcaagg cgttgttcgc cgagcgcatc agcgcacgga
     5161 ttgtcgatct cgacactggc accttcagcg atgcgcagaa tccggccact ttcgatccgt
     5221 tgaaagcgtc caaggatccg aagttgtcgg ctgtgcttga aggctccgac gatatcgcct
     5281 cgatcaagcg ccgcgagcgt ttcaccacgg tttacctggt ggagcaggat ggcaagctgg
     5341 agaccgtgat tctgcctgtg cgtggctatg gcctgtggtc gaccctgcac ggttttatgg
     5401 cgcttcaggg tgacctcaat accgttgccg gtttcggctt ctaccagcat gccgagaccc
     5461 cgggtctagg cggcgaagtg gataacccga agtggaaaag tctgtggctg ggcaagacct
     5521 tgtttgatga caagggcgag ctggcgattc agatcatcaa gggcaacgtc gatgcgcaga
     5581 gcgcgcaggc gactcaccag gtcgatggcc tggcgggtgc aaccctgacc agcaatggtg
     5641 tgaataactt gctgcacttc tggctgggag agaacggctt cgcccagttc ctcgccaatc
     5701 tgcaaaaagg ggaggcttga tcatgtcgca gccgactatt aaag
//
LOCUS       DASRUM010000018         3805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1516037, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000018 DASRUM010000000
VERSION     DASRUM010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516037"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="VFF22_01280"
     CDS             <1..1222
                     /locus_tag="VFF22_01280"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HZX15486.1"
                     /translation="EQAAAVVRYQGGHNAGHTLVIDGEKTVLHLIPSGILRENVQCLI
                     GNGVVVAPDALMREIIKLEEKGVPVRERLRISPSCPLILPYHVALDQAREKARGDAKI
                     GTTGRGIGPAYEDKVARRGLRIGDLFHRERFAAKLGELLDYHNFVLVNYYKEPAIDFQ
                     KTLDECMEYAELLKPMMTDVTAVLHDMRREGKDIMFEGAQGSLLDIDHGTYPYVTSSN
                     TTAGGIATGSGFGPMYLDYILGITKAYTTRVGSGPFPTELFDETGAFLAKRGHEFGST
                     TGRARRCGWFDAVILRRAIEINSISGLCLTKLDVLDGLETIRICVGYKDQNGDVLVDA
                     PTDADSYLGLQPVYEELPGWSESTLGAKTLEELPAAARTYIKRVEELVGAPIDIVSTG
                     PDRNETIVLRHPFA"
     gene            1360..3294
                     /locus_tag="VFF22_01285"
     CDS             1360..3294
                     /locus_tag="VFF22_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX15487.1"
                     /translation="MFAIISVLRSRLLLPVFLALGVALLVQVVVAVALTRTTVATLEA
                     DLAKGMQADTARLVDELAKAGDEVRGGLDSLSASTQAQLAKGLSARLAEEQAQIRGVL
                     DSNLKRSADELAQLLSAVAPKAIWDADVPALTELARGAHRNPAVLFAVYLDAQGARLT
                     RHLNRQDSRVRALMAKGQGDGALDKVLQAAASDPGVYIAEATISPMGSDIGKVLLGVS
                     TASVDEELAKLDQRFVSLIDSSGQLVSDSLSGAATSSADALAARLSVAREAAGGMEQS
                     SREVVQQAAAGLRWNISMGLVVVAVLILLVLALVLGRRVVSKLHTLIAVLNDWAAGEG
                     DLTRRVELNSRDEIGDMAAAVNRFVAKLQPIVREAGEVASLTGQEIGNLASRSAAAES
                     AAERQRDEVAGSLQALAEMAGEAQAESHAMREALQRVGAIRQAADDNAAIALRVGGSI
                     EELVRRVETGSAVIERLAKQSEQIEVVLTVIRSIAEQTNLLALNAAIEAARAGESGRG
                     FAVVADEVRALASKTQQSTGDIQTHIGALQQGAREAVEAIGQAGKQADQGLAALRESA
                     RVQQSVREAVEEVHGAINTATEAAAHQAEGADAVRGRVEVIHREAQRAAEAVAATANS
                     GRVLDGLAKKLKASLGQFRV"
     gene            complement(3400..3486)
                     /locus_tag="VFF22_01290"
     tRNA            complement(3400..3486)
                     /locus_tag="VFF22_01290"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3450..3452),aa:Leu,seq:cag)
BASE COUNT          710 a         1011 c         1277 g          807 t
ORIGIN      
        1 cgaacaggct gcagccgtcg tgcgctatca gggcggtcac aatgctggcc acaccctggt
       61 gatcgacggc gaaaaaaccg ttctgcacct gatcccctcc ggcatcctgc gcgaaaatgt
      121 gcagtgcctg atcggcaacg gcgtggtggt tgcgcccgat gcgctgatgc gtgaaatcat
      181 caagctggaa gagaagggtg tgccggtgcg tgagcgtctg cgtatcagtc cgtcctgtcc
      241 gctgattctg ccgtaccacg tggccctcga tcaggcccgt gagaaagccc gtggcgatgc
      301 caagatcggc accactggcc gtggcattgg cccggcctac gaagacaagg tggcgcgccg
      361 cggtctgcgc attggtgatc tgttccaccg tgagcgcttc gccgccaagc tcggtgagct
      421 gctggattac cataacttcg tactggtcaa ttactacaaa gagccggcca tcgacttcca
      481 gaaaacgctc gatgagtgca tggaatacgc cgagctgctc aagccgatga tgaccgacgt
      541 gaccgccgtg ctgcacgaca tgcgtcgtga gggcaaggac atcatgttcg aaggcgctca
      601 gggctcgctg ttggatatcg accacggcac ctacccctac gtcaccagct ccaacaccac
      661 agcgggcggt atcgctaccg gttccggttt tggtccgatg tacctggatt acatcctcgg
      721 tatcaccaag gcctacacca ctcgcgttgg ctcggggccg ttcccgactg agctgttcga
      781 cgaaaccggc gcgttcctcg ccaagcgtgg ccacgaattc ggctccacca ctggtcgcgc
      841 gcgtcgttgc ggttggtttg atgccgtgat cctgcgtcgc gccatcgaga tcaacagcat
      901 ctccggtctg tgcctgacca agctcgacgt cctcgacggc ctggaaacca tccgtatctg
      961 tgttggctac aaagatcaga acggcgacgt gctggttgat gcgccgacgg atgctgacag
     1021 ctacttgggt ctgcagccgg tttacgagga gctgccaggt tggagtgagt cgaccctggg
     1081 tgccaagact ctggaagagc tgccggcggc tgcgcgtact tacatcaagc gtgtggaaga
     1141 gttggtcggt gcgccgattg atatcgtttc caccggcccg gaccgcaacg agactattgt
     1201 gttgcgtcat ccgtttgcct gatagtcaca ggcagtgcac aaagggtcgc caattggcgg
     1261 ccctttgtcg tttctggtcc aggtaaaaat atccgagcta gcctttataa atcagcttcc
     1321 ccgccgttat aggcattacc gctgttagga gtgtctgccg tgtttgccat tatttctgtg
     1381 ctgcgcagtc gtttgttgtt gccggtgttt ctggcgctgg gtgtggcctt gctggttcag
     1441 gttgtcgtgg cggtggcgtt gacccgtacc acggtggcaa cgcttgaggc tgatctggcc
     1501 aagggcatgc aggctgatac ggcgcgtctg gtggatgagc tggccaaggc cggcgatgaa
     1561 gtgcgcggcg ggctggacag tctgtctgcc agcacccagg cgcagttggc caagggcctt
     1621 tccgcgcggc tggctgagga gcaggcgcag attcgcggcg tgctggacag caacctcaag
     1681 cgttccgctg atgagttggc gcagctgcta tcggctgttg cgcccaaggc aatctgggat
     1741 gccgatgtgc ctgcgctgac tgaattggcg cgtggcgcgc atcgcaatcc tgctgtgttg
     1801 tttgcggtgt atctcgatgc acaaggcgcg cggctaacgc ggcaccttaa tcggcaggac
     1861 tcccgcgtgc gtgcgctgat ggctaagggc caaggcgatg gtgcgctgga taaggtgctg
     1921 caggctgcag ccagtgatcc gggcgtgtat atcgccgagg cgacgatcag tccgatgggg
     1981 tcggatatcg gcaaggttct tttgggggtt tccacggcgt cggtggatga ggaattggcg
     2041 aagctggatc agcgtttcgt cagcctgatc gacagcagtg ggcagttggt ttccgacagt
     2101 ttgagtggtg ccgccaccag cagtgcggac gccctggcgg cgcggttgag cgtggcgcgt
     2161 gaggctgccg gcggtatgga gcagagcagt cgcgaggtgg tgcagcaagc tgcagccggt
     2221 ttgcgctgga atatcagcat gggcctggtg gtggtcgcgg tgctgattct gttggtgttg
     2281 gcgctggtgc tggggcggcg agtggtcagc aagctgcata cattgattgc ggtactgaat
     2341 gactgggcgg cgggtgaggg tgatttgact cgccgggtcg agctcaatag ccgcgatgaa
     2401 attggcgata tggcggcggc agtcaatcgc ttcgtcgcca agctgcagcc gattgtgcga
     2461 gaggcgggtg aggtggcatc gctcaccggg caggaaatcg gcaatctggc ctcgcgcagt
     2521 gcggcggccg agtcggctgc cgagcgtcag cgcgatgaag tggcgggcag tctgcaggct
     2581 ttggcggaaa tggccggtga ggcgcaggcg gaaagtcacg ccatgcgcga ggcgttgcag
     2641 cgcgtcggtg cgattcgtca ggctgcggat gacaatgccg ccattgcgct gcgcgtgggt
     2701 ggctcgattg aggaattggt gcggcgtgtg gaaaccggct cggcggtaat cgagcggctg
     2761 gccaagcaga gtgagcagat tgaagtggtg ctgacggtga ttcgctcaat tgccgagcaa
     2821 accaacctgc tcgccctcaa tgcggcaatt gaagcggcgc gagcgggtga aagcggtcgc
     2881 ggttttgctg tggtcgcgga tgaagtgcgc gcgctggcca gcaagacgca gcaatccact
     2941 ggcgatatcc agacccatat cggcgcgctg cagcagggcg cgcgcgaggc ggttgaggcg
     3001 attggtcagg cgggcaaaca agcggatcaa ggtctggcgg cattgcgcga aagtgcacgg
     3061 gtgcagcagt cggtgcgtga ggcggtggag gaggtgcatg gtgcaatcaa caccgcgacc
     3121 gaagcagctg cgcatcaggc tgagggtgct gatgccgtgc gtggccgtgt cgaggtgatt
     3181 catcgcgagg cgcagcgggc ggcagaggct gtggcggcga cggccaatag tggtcgggtc
     3241 ttggatggtt tggcgaagaa actcaaggcc agcctggggc aatttcgcgt ttaagccgat
     3301 tcaggacggg cgcgtctagc gcccgtccaa ttggttgcag cttgctgggc taaatcacag
     3361 gcaataaaaa accgagccag cggctcggtt tttttgaatt ggtgcccagg agaagactcg
     3421 aacttccacg accgtaaggt caccagcacc tgaagctggc gtgtctacca atttcaccac
     3481 ctgggcattg attgcaaaaa atgcttattt gctatcaact acaactcggt taacttcgtt
     3541 tcccgtcgtt gtggcgcgca taatacggac cgtgttttta cttgtaaacc cctgttgtta
     3601 aaaaatttta ttctgtgtgc cgggctgacc tggacagtgg tttcgcgctt caataggcac
     3661 atggcaaacc gaatttgtgt agataaggtg aattacctct aatggccgat tggcaatccc
     3721 tcgatcccga ggccgctcgt gaagcggaaa aatacgaaaa ccccattcca agtcgcgagc
     3781 tgatcctgca gcacttgtct gaccg
//
LOCUS       DASRUM010000019        33209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2186318, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000019 DASRUM010000000
VERSION     DASRUM010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 33209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33209
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2186318"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VFF22_01295"
     CDS             <1..992
                     /locus_tag="VFF22_01295"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HZX15488.1"
                     /translation="AAQLAPTASPLPAEATTALALVPDRDWRLALRTATTTGGAALAC
                     AFLAQAYPSQATELAILGVCIFSMLLGSMAQPQKQVPHMLLGVLAGVTGATLYRFAVQ
                     PHVDGMLELVLTLLPFLLVGGILRASRRFAVAAIDANMCFMLASQAGMPTASSGAIIS
                     SSLALLLAAGLVGSGFLLFPSTVQRALRLTTQAILGDIRTLLRDSNARSRERWLALAG
                     RRILRLLAHMGTDTHSGTTPPASLLSLLNLGQSIIDLHHLGQQPGHRAIYQEAIARLA
                     DFERQPQATAEFLLHLAEDSRDRWLTVTLQTLAHALLASRSTLLAQRPSISSA"
     gene            complement(979..1995)
                     /locus_tag="VFF22_01300"
     CDS             complement(979..1995)
                     /locus_tag="VFF22_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003201580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HZX15489.1"
                     /translation="MFLRDNGRDLAEALCQQIANLNAGRGGMLPGSSLLARYPQLAKG
                     LEQLAGLMQQTWDETTGLHEQVQRLCEERDQALAREQQLRDEMLHQQMTLAAVQAQLH
                     EQQQVLSSCELEARIWEVAQSTLTEGYWDLSVANGKVDDPGSMMRISNQFRVLMGYRL
                     DELPDGWDSQVGITHPEDLNGIFNLFERQIVDPAGSGEYVVEYRMRHKTRGYIWCRER
                     GRAVRDEQGRLYRVIGAVRDISDERNAKAAHEQMLQHNQQTYGQIARVVSVIKGIAEQ
                     TNLLALNAAIEAARAGEVGRGFSVVADEVKKLAQSTRQATQDIQSMLGDAQARTQQLT
                     ATPS"
     gene            complement(2002..3087)
                     /locus_tag="VFF22_01305"
     CDS             complement(2002..3087)
                     /locus_tag="VFF22_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZX15490.1"
                     /translation="MRGLLCAVLIFCSVSGYAQTLRIYNWKDYINPQVLAGFERDTGI
                     RVDYQTFTTAQELDEALDAGAGFDLVAPSHFQLQRLIGGGRLQALDVGKLRHYGNLDP
                     KLVASLAGFSGAGRYVVPYLWSTVGLVVEREAVERALGEAAPASWSLLFDAQYAQRLQ
                     ACGMGWLDAPEETFSLWMNLQGKRLFEASPRRLADSGAQLASQAAALHALNNEAYIEE
                     LAAGRLCVAMAWSGHALLAASQRNTLEFLVPEEGALMTIDSWAIPRDAQQPELAYRFI
                     DYMLEPGNARLNTLATRFYAPLRSDLAEMIKLAQSAPALVPQASERRRLYFLEPLKAE
                     QKRVIDQQWQAVKQHQRLHQPLSTELE"
     gene            complement(3234..4580)
                     /locus_tag="VFF22_01310"
     CDS             complement(3234..4580)
                     /locus_tag="VFF22_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZX15491.1"
                     /translation="MQHKKLGFITMMAICVGLVIVQGSMITATQGIGIGGMGFIAALF
                     VAFILAQFNAMSFAELSLMFPQEGTLATYTQKAIGHFPAIVAVFAGYVVVAMLALPVE
                     MFLVDIIIGELLPGMFPPLVLPLLILGFFVVTNILGADVFAKVQNLLAFILVTALILI
                     GLCAVTGAVEPHAELAGTAVSWSFDPVLDGSFIGLVAMAMWLMIGVEFICPMINEVEN
                     PQRNIPRAMMLSLCAMLGIFLLFVYGVTHYLDIETLTTSPLPYLDYANAVFGKAGLII
                     AAVMAVTATCSTVNTVLAAVPRMLQGMAENGQAFPQMKITSKRFGTPWVGILFMAAII
                     IIPLLAMDIDSLIPLVIAASTSWLLAYVVAHVDVLVLRRRLPHQARPFKTPFYPLPQI
                     LGTAAMLYVALNNSPSPEITVMVYKITGGILLLVSVIAALWVKLYMKRKLFEPDLG"
     gene            4862..5548
                     /locus_tag="VFF22_01315"
     CDS             4862..5548
                     /locus_tag="VFF22_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017001431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX15492.1"
                     /translation="MQNVLITGASRGLGLGLTRAYLARGARVYAVIRRQPSGELLALS
                     QGHAATLRLILCDLDDDQAAARIGEALQGTRLDRALFNAGIYGPAHQDPCQVSHEEIA
                     QLFLSNAIAPVRLAQALLPSIREDGVLAFISSQMASVELARATDMPLYGASKAALNSL
                     LRSWSQVPDLSPVTLLALHPGWVRTELGGPDAPLSVEESARGLIEVIEAQAGQPGCRF
                     LDHQGHVLPW"
     gene            complement(5767..6705)
                     /locus_tag="VFF22_01320"
     CDS             complement(5767..6705)
                     /locus_tag="VFF22_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HZX15493.1"
                     /translation="MTSTTAALDPRAFRRALGNFATGVTIVTAATADGQKVGVTANSF
                     NSVSLDPPLVLWSIDKRSGSYEVFEQAAHFAVNILSADQIDLSNQFARPSDDKFAGVT
                     HELGEGGAPLLPDCAARFQCEKHQQIDGGDHWILVGKVLAFDDFGRSPLLYHQGAYSM
                     VLPHTRMTEKTEGQQPSSAFQGRLSHNLYYLMTQAVRSYQSNYQPRQLSTGLRTSEAR
                     MLMVLENDAGLSLCDLLREVAMPVREIEQAVDILKRKGLVADADDRYVLTPAGVDQAE
                     ALWRIAGEQQDKVFGAFSEEQIDTFKAMLKAVIAIG"
     gene            complement(6795..7964)
                     /locus_tag="VFF22_01325"
     CDS             complement(6795..7964)
                     /locus_tag="VFF22_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent monooxygenase"
                     /protein_id="HZX15494.1"
                     /translation="MKKPNPLLEDLKAILPAIAANAAQAEKDRKAPDENIALLKGIGM
                     HRAFQPKPYGGMEISLPQFADCVVALAGACASTAWAFSLLCTHSHQLAMFAKQLQDEV
                     WGDNPDATASSSIAPFGKVEEVQGGVIFNGEMGWSSGSDHAEWAIVGCRRQNDEGGQD
                     YCFAVLPRSDYEIRDDWFAAGMKGSGTKTLIIKDVFVPNHRIQKAKDMMEGKSAGFGL
                     YPDSKIFYTPYRPYFASGFAAIGLGIAERMLEAYREKTKNRVRAYTGVNVGTATPALM
                     RLAESTHQVAAARAFLEKTWQEHAEHGERHQYPSRETLAFWRTNQAYAVKMCIQAVDR
                     LFEAAGGTSWFESNEMQRLFRDSHMTAAHAYTDYDVCAQILGRELMGLEPDPSMV"
     gene            8252..9454
                     /locus_tag="VFF22_01330"
     CDS             8252..9454
                     /locus_tag="VFF22_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX15495.1"
                     /translation="MSHLPSTLRALRHRNFRLYFIGHAVSTLGTWIQQVALAWLVYRL
                     TGSVALLGLTTCMSLLPQLLVGPFAGAWIDRHDKRRLLILVESLLGVQALALALLTAG
                     DWVTPTLLVAMATLMGVLNALDTPLRQSLLSQFVDDRADLPNALALNAMLFTSSRFIG
                     PPLAGLLLAVFSEALCFALNGISYLALVIGLLCIRIAPTARAAGSYRNVLSEGLRYAL
                     GNRQVRAMMLSVLAINVSASSYVVLLPVFAKDIFAGGATTLGWLWGAAGLGSLLSTVF
                     LASRLAVDSLPRLILAASCATALALLVFAWSDLLALSLAAMAVIGFGITVSNVGTNIV
                     LQSNAPEALRGRIVSLYTSTRFGFDALGGLLAGLLAGYLGAPPVMAGAGVALLLYCIC
                     VELQRRRN"
     gene            complement(9780..10367)
                     /gene="cysC"
                     /locus_tag="VFF22_01335"
     CDS             complement(9780..10367)
                     /gene="cysC"
                     /locus_tag="VFF22_01335"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009043426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HZX15496.1"
                     /translation="MSGSGKPDYRHKGQVSREQRESLNAQRGQVLWLTGLSGSGKSTL
                     AFALEARLYKLQRRCVVLDGDSLRGGLCADLGFTPDNRHENLRRAAEVAKLFAESGLI
                     CIAAFISPTSRDRAMVRDIVGANDFSEVYLNCSLQVCEARDVKGLYRRARAGEIADFT
                     GITSPYEPPQQPTLTLMTSSLSIEDCVQQLTECVG"
     gene            complement(10357..11574)
                     /locus_tag="VFF22_01340"
     CDS             complement(10357..11574)
                     /locus_tag="VFF22_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZX15497.1"
                     /translation="MWSDRFSQDDDARNQARVSKLQNSHRGRQGWLLMAVLIAVVTFA
                     IWASLFRIDEVARATGEVIASSRVQIIQSVDGGVLEQLLVKEGDRVAPGQLLARLEQT
                     RLGASVGEVEARLFGLQARATRLRAEVIGERRLSFQADLLERSPETARVEEALFKQRQ
                     LGLEEELRTMRVAVSLAQKELRLVEQLYANGDASGSELLRVQRGLNEAEARLVNRRNK
                     FLEDARLELAKAEDDISQSGQTLTLRKQEQQDSVFTAMVPGIVKNIRVTTVGGVLRAG
                     EELMQIIPVDDDLMIEAKVSTADIARVVPGLEATVRFDTFDYTIFGGVKGEVAFVSAD
                     SLKEETSRGMDIYYRVRIKPASYPVTSTTGRVLDVLPGMTAQVDIRTGERTVMDYLLK
                     PLRKTLSESFGER"
     gene            complement(11564..13612)
                     /locus_tag="VFF22_01345"
     CDS             complement(11564..13612)
                     /locus_tag="VFF22_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZX15498.1"
                     /translation="MDHTFAAVKPAEWLRQVLVQAGLKNLVPVQLAWRRLDLRRLPAL
                     VCTDDGWFLAVREATDAVTLINAAGERREVHDTELQDALVVWLRAPTSWNADAGRAFS
                     GNLATRLVWRELFRERGWLWKVMVATVLVNLLAVGTSIFAMQVYDRVVPTLAYATLTT
                     LVTGMAMIILLDWLLKTTRARILDSLSCAVDKRVSQQVFEHLLHLRLDQQPRSLGTLA
                     AQVGGLDTVRQFFSSGVVFALVDLPFALLFLAFIAIIGGAVGWVYAVLLPVALLLGYV
                     TQWRLRALLKQQMLRSNERQGLLVDSIRGAESIRANNAGWRFAQEWQSITASIDGYSI
                     QQRAISSFSSVSTSSLSTLAYVAAVVVGVWQIEAGLLTMGGLIACSILGGRVIAPIAQ
                     SVQYLAQWQHVRQALQMVHQVLSLERDRRPDQHLLLPDELPNEIALDRVRFAYPESPV
                     CQVNIEGLRFTSGERVLLVGPVGCGKSTLLKVLAGLYRPSEGRVRLGDADLWEIDPQL
                     VASQVGYLPQTVQLFKGSLRSNLALTGANSDSRLLQITRELGIDAIAASSPQGMDLSI
                     SEGGEGLSGGQRQLVALARVLINQPRIWLLDEPTAALDGESEQRVWAALQEAVRPEDI
                     LIVATHRPMAATRLATRVIVMQQGQVTKDGTPDKVLAQMLARPVASSRMGKGGTLDVV
                     "
     gene            complement(13737..15026)
                     /locus_tag="VFF22_01350"
     CDS             complement(13737..15026)
                     /locus_tag="VFF22_01350"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZX15499.1"
                     /translation="MVISLPRLGRRSALALCLGVLALPVNAQQADGLQAALQAALTLH
                     PAISGKQSQIKAREYSADAARAQRYPSLTAQAQQYSGDNRDATTGEDLSTPVTLRARQ
                     PLWAFGRIDNSIAFADADTDVERVDLLRVQRQLLEQTAVAYASVVGSRQRLQVVEASL
                     AAHQSLFDQISRREQGQLASTADVRLAATRLSQSKARVERYAGELEIAESDLLALTQV
                     RLPAQLPVASGLLHVTEGEALRQAALQGSAEVRFKKQEIERAKAGVDQAKTASMPTVY
                     LQAEQDYDQPSYRDDTRVSLVFEGTLEGMGFATRGRTGAAVAQQSAAEDDLTYAVNEL
                     ERSVRRLQRNRELQAELIQVQGESLTELEAVLGSYQRQYAAGTKSWLDVLNIQRELSE
                     QQLQLVQAQSDWVIYSLQLAALTGGLDALVALNTEEL"
     gene            complement(15020..17653)
                     /locus_tag="VFF22_01355"
     CDS             complement(15020..17653)
                     /locus_tag="VFF22_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZX15500.1"
                     /translation="MSSLPLLRRLVEQRQFELLFEACSDSSPQHPAEHVLWTLACAHL
                     GDAQAARQALSVLDVSALDLEARVDLAAVHLLLGDTDAAQVLLTDALVDAPEHSLLLA
                     RLAACHLQRQEPEVARQLYEQSLALQPRILVFEHLLHLYLQSADLARAEHTWAEAQAF
                     WAGEQAHWPEAQAAWHTQQLRGLRLDLWLANEGFAAADAWLDAQQVVLEEEPWCDLLG
                     GYALRLTERGRHAQAEDALRRGLRHYPAQQGLLQQLAQLAIVQGRMPQAIALLRRALR
                     MTSQEQKPTLALRVKLASMTTQNYPALAREMAQQAIEEALALQPSKDVSPAQIAHWLA
                     QTQVALAGVETQEQQYVEAEARYREVLDAHPQLLIALQGLGHLYMQLGRIDDAIELFE
                     QVKALDPVRGHAALINARRFPDDDATLQRLEAVARRPGLEGPVQPGLLLQLAAAREKR
                     KDYAKAFALADEANSASRKLLSYDPAAHRQRCARIRHAFNRDLYEHRPGVGNNSTLPV
                     FVLGMPRSGTTLIEQMLAGHSQIHGAGELGVMPSVIAGLERWERHVGSGRHYPDCVDD
                     LSAKVVAGISANVLKELQEYAPEALHVVDKLPHNFENIGLIKLLFPNARIISVRRDPR
                     DIAISNYFTDYAAKHGGMGFAYDLGWIGEQLADHNLLMHHWNQVFPGDILEVRYEDVL
                     ADPERETRRMLDYVGVGWEPQVLNFSELQRPVKTASVWQVRQPLYTTSMAKWARYEAY
                     LAPLIRGTNARIEWQPITEMVALPVPGLLETGVAFYREEKLDDAEYHFKQLLHHLPEH
                     AAATFMLGLIDLRKGHVSEGIALMLKAHEQCPWNGLWRDDLARAYELSGEPEKAAALK
                     PKKPHASVPDPEQEMDGEFAW"
     gene            complement(17730..24095)
                     /locus_tag="VFF22_01360"
     CDS             complement(17730..24095)
                     /locus_tag="VFF22_01360"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007156 - homophilic cell adhesion via
                     plasma membrane adhesion molecules [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin domain-containing protein"
                     /protein_id="HZX15501.1"
                     /translation="MGNWANIWNKKIIARRAGREFCMTSEMAVKQSMQLVERLADGSM
                     RYIPLIDMVSVPAKSGAQYALIDITTGKSPEGLQVKRKGSSLIVESEDDGFLVEITEF
                     YDEPETAFIPQAELDGSALVGTSVTSETPVIDTAANGDEIIWRGGSDSDLVTPTNLVL
                     GGLGLVGLGLAAGGGGGGGSSGGGEAPSSVLNTVNGTIVGGPVVAGNDLRVEVYQADG
                     ITLLGEAQINADGTFSIPVGSYLGVVIARVVNAGLNADYLDEATEAGKDLNAELFAME
                     VVSQPNSTITVNINVLSTLAYHKVQDAAAGGLPTAALVDSTNDAIAELFGLPNLHGTA
                     VVPTNGGSFESNDGLTSGEIYGAVLAALSGADAQNGGDSQQTINDLLAGLDVSDGTAT
                     LTESAQGIVVQGGQFTSTQTGQDLTGAVAGVVDTYAPDFTSGATATAINENSSAGQIV
                     YTAAASDAGSVVSYSLKAGDDAALFSINASTGAVTLTGNPDFETKPSYNFTVVATDVA
                     GNASEQVVTLGINNLDDTAPTITSAATASPINENSSAGQVVYTVTSTDSADVVSGATS
                     YSLKAGGDAALFSINTATGAVTLIGSPDYETKASYSFTVVATDAANNASEQTVTLAIN
                     DLVDETAPAVSSIAITSASGAQNSTLNAGDVVSVTVNMDEASLVDITGGTPRVALNIG
                     GSTVYADYVSGSGSTALVFQYSLQAGQTDANGISLDANALQANGGTLRDAAGNAANLA
                     HGAVADNASYLVDTTAPAISSGATATAIDENSGAGQTVYTAAATDAGSLTYSLKAGGD
                     AALFTIDVGTGAVTLIGNPNYESKTGYAFTVVATDAAGNSSEQAVTLGINNLDDTAPT
                     ITSAATATAISENSGAGQVVYTVTSTDTDDVVSGATSYSLKPGGDSALFSINAVTGAV
                     TLTDSPDYETKASYSFTVVATDVAGNFQEQVVSLAINDLVDEVAPTVSSIAISAATGA
                     QNNSLNAGDVVSVTVTMDEATLVNTAGGTPRVALSVGGSTVYADYVSGSGSTALVFQY
                     TIEAGQTDANGISIATNALQAGGGTLRDAAGNAADLDHGAVADNASYLVDTTAPVISS
                     GATATAIDENSGTGQIVYTAAATDAGGVTYSLKADGDHALLSINASTGAVTLTGNPNY
                     ESKTNYAFTVVATDAAGNAIEQTVTLDINNLDDTAPSITSAATATAINENSGAGQVVY
                     TVTSTDNDDVVSGATSYSLKPGGDSALFSINAVTGTVTLTDSPDYETKASYSFTVVAT
                     DTAGNFQEQAVTLAINDLVDEVAPTVSSIAISAATGAQNNSLNAGDVVSVTVTMGEAT
                     LVDTTGGTPRVALNVGGSTVYADYVSGSGSTALVFEYTIQVGQTDGNGISLDANALQA
                     NGGTLRDAAGNAADLDHGAVTDNASYLVDTTAPSFNSGVTATAIDENSGAGQAVYTAA
                     ATDAGTVTYSLKADADAALFSIDTGTGAVTLTGNPDYETKPSYGFTVVATDAAGNASE
                     QAVTLAVNDLDEVAPTVSSVAISGATGAQGSLLNAGDVVSVTVTMDENTIVTGTPRVA
                     LDIGGSTVYATYVSGSGSSALVFQYVIQASQTDVNGIAIPLNALELNGGTLKDAAGND
                     AVLAHGAVADNASYQVDTTAPSVTSGAVAAAISENSGAGQTVYTAAATDANTVTYSLK
                     VVGDHAAFSIDGVSGAVTLTGNPDYETKASYSFTVAATDAAGNTTEQAVSLSINDVDD
                     TGPAVSSVAITSATGVQNNTLNAGDVVSVTVGMNEATLVNTAGGTPRLALTIGSSTVY
                     ADYVSGTGTASLVFQYTILASQTDPNGISIAANALEANGGTLRDATGNDAVLTHALVA
                     DHASYLVDAAAPTLSSSAPTDNETDIAIGNNITLTFNETVLAGSGNIVISNGTDTRTI
                     SISDATQVSISGSIVTINPTLDLERGSTYNVQMAAGVLTDQAGNAFAGINDDTTLNFD
                     TETVDSSIVVFDLVQGVSSDHSGRTFDANTSYTIFIRVDSNSHVLSTAGSGPGTWDTW
                     SGANNLGVDDRIVLVGTGTPVKGTAGFGVTYLLGNPNTLIWASSGYAVELGLDGQLKR
                     SNGISAVMDIWGGSWAANPNTEHLGAVGANVYLTQIPAGILTSQGLV"
     gene            complement(24498..25400)
                     /gene="hpaA"
                     /locus_tag="VFF22_01365"
     CDS             complement(24498..25400)
                     /gene="hpaA"
                     /locus_tag="VFF22_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HZX15502.1"
                     /translation="MRDPQPIPNINIGQVYDQRYADADVHYEALGKLADFFGRNMPVH
                     RHDRFFQVHYVKSGVVRVYLDDQRYQQEGPMFFLTPPTFPHAFVTDADSDGHVLTVRQ
                     ELIWPLLEGQQGLALGPQIAPLCVAVGELDAQYDTEVARLNLLFDELRSEFADDRPGR
                     ALSLAALTRLIFISLLRLSTRSIQSQPARREDLQIFQRFNALIEANYQAHWALQRYAE
                     QMGVTEARLNDICRRIADLPSKRLVFDRLMQEAKRLLLFTGSSVNEICYQLGFTDPAY
                     FSRFFTRNAGMTPGEYRVTKGVAD"
     gene            complement(25460..25882)
                     /gene="hpaR"
                     /locus_tag="VFF22_01370"
     CDS             complement(25460..25882)
                     /gene="hpaR"
                     /locus_tag="VFF22_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HZX15503.1"
                     /translation="MPVTRPSLTLTLLQAREAAMSFFRPLLNQHGLTEQQWRVIRILR
                     QHGEMESYRLAELACILKPSMTGVLSRLERDGLVSRSKPPHDQRRVYIDLTAQGHECF
                     VSMSADMESNYQRIQEQFGEEKLQQLLGLLNELKQIKP"
     gene            26080..27579
                     /locus_tag="VFF22_01375"
     CDS             26080..27579
                     /locus_tag="VFF22_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009621649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZX15504.1"
                     /translation="MTAALIETLKSVEDFLQRQHGSFIDGTCQLDSASETLALRNPAD
                     DQVLANVQLADAGQVDRIVESAHRAFRSGVWSRLAPAERERTLLRFADLVEAHGEELA
                     QLETLNQGKSIHLARAIEVGASVNYMRYMAGWATKIEGQTLDVSIPMPANARFNAYTR
                     REPTGVVAGIVPWNFPMMIALWKVIPALACGCTIVIKPADETPLTALRLAELAIEAGI
                     PAGVFNVLIGRGAQAGAALVAHPLVNKVSFTGSTAVGKQIGIAALHNMTRFTLELGGK
                     NPMLVLEDADIDKAVAGAIAGGLLNQGQVCAAASRLYVPASRHAQFVEALSAQASAMS
                     LGPGMDPTAQVNPLATRKHQNHVLAMLALARRDGARIATGGDAPDRPGYFVNPTVLSG
                     LEQRSSTVQEEIFGPVLSVLPYDDLEQGLALVNDSRYGLAASVWSNDLGKVMDLLPRI
                     EAGTVWVNNHVPVDPNLPFGGFKQSGMGREFGRSAIEGFTELKSVCITY"
     gene            27871..28539
                     /locus_tag="VFF22_01380"
     CDS             27871..28539
                     /locus_tag="VFF22_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009621682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZX15505.1"
                     /translation="MSSALDKAAAGTLFGIALNYQGLLRSRLEEFQRPPYQKPPVKPV
                     LFIKTSNTRNGHDQPVVFPAGVERLQPGPALGVVIGKDASRVSEAEAMNHVAGYLIVN
                     EFSLPEDSYYRPAVKAKCRDGFCPIGPELVPASQITNPNALGIRLLVNGELRQQNSTA
                     NWVRNIPQLIAELSEFMTLHKGDVLITGTPEGRVDVQPGDIVAVEIDGLGRLVNTVVA
                     EGAL"
     gene            28539..29327
                     /locus_tag="VFF22_01385"
     CDS             28539..29327
                     /locus_tag="VFF22_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZX15506.1"
                     /translation="MKHARIRFQGDIHAVTVEDNNAVRLADGRMLAEEAVEWLPPASG
                     SMFALGLNYADHAAELAFSPPTEPLAFIKSPGTYTGHNQITWRPDNVAYMHYECELVA
                     VIGKPARNVKREDALEYLAGYTVCNDYAIRDYLENYYRPNLRVKNRDCTTPVGPWIVD
                     VADVPDPSKLRLRTWINGELKQEGTTSDMIFDIPYLIEYFSSFMTLQPGDMIATGTPE
                     GLADVVPGDEVVVEVEGVGRLVNRIVSEADFFTQQELARNKQEA"
     gene            29331..30791
                     /gene="hpaE"
                     /locus_tag="VFF22_01390"
     CDS             29331..30791
                     /gene="hpaE"
                     /locus_tag="VFF22_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HZX15507.1"
                     /translation="MIKHWINGREVESKETFTNYNPATGEAIGEVASGGAEEINQAVA
                     AAKAAFPKWANTPAKERAKLMRKLGELIEQNVPKLAELETLDTGLPIHQTKNVLIPRA
                     SHNFDFFAEVCTRMDGHSYPVDDQMLNYTLYQPVGVCGLVSPWNVPFMTATWKTAPCL
                     ALGNTAVLKMSELSPLTANELGRLALEAGIPEGVFNVIQGYGATAGDALVRHPDVRAI
                     SFTGGTSTGKKIMQTAGLKKYSMELGGKSPVLIFDDADLDRALDAALFTIFSLNGERC
                     TAGSRIFIQETVYDQFVAEFAARAKRLIVGDPQDPNTQVGSMITQAHYDKVTGYIRIG
                     IEEGANLLAGGLERPANLPAHLAKGQFIQPTVFADVDNRMRIAQEEIFGPVVCLIKFK
                     DEAEALRLANDTEYGLASYIWTQDIGKAHRLARGIEAGMVFINSQNVRDLRQPFGGVK
                     GSGTGREGGEYSFEVFAEIKNVCISMGSHHIPRWGL"
     gene            30920..31843
                     /gene="hpaD"
                     /locus_tag="VFF22_01395"
     CDS             30920..31843
                     /gene="hpaD"
                     /locus_tag="VFF22_01395"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012273111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HZX15508.1"
                     /translation="MGKVALAAKITHVPSMYLSELPGPRQGFRQAAIDGHIEISKRCR
                     ELGVDTIVVFDTHWLVNANYHVLCGPHFKGLYTSNELPHFISNMEYEFPGNPALGKLL
                     ADECNRFGVETMAHDATTLGPEYGTLVPMRYMNQDQHFKVVSVSALCTSHYLNDSARL
                     GWAMRKAVEEHYDGTVAFLASGSLSHRFAQNGQAPDFATKVWSPFLETLDHRVVQMWQ
                     DGEWDAFCGMLPEYAAKGHGEGFMHDTAMLLGALGWSNYDAKAEVVTPYFGSSGTGQI
                     NAIFPVIPQDGSAIPAAQASNPAGVVSTSRL"
     gene            31954..32364
                     /locus_tag="VFF22_01400"
     CDS             31954..32364
                     /locus_tag="VFF22_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate isomerase"
                     /protein_id="HZX15509.1"
                     /translation="MPHLVLLYTPDIERDLDVSGLCRALADCMLEQRDEAGKAVFPTG
                     GTRVLAYPAAHAAVADGKGDYGFLYANLRMGAGRSQVVHKQVGDNLMHVLRARLDGLL
                     AQRPLGVTLQIDESPGQVFDAKHSSLHPLFNKAP"
     gene            32377..33180
                     /gene="hpaH"
                     /locus_tag="VFF22_01405"
     CDS             32377..33180
                     /gene="hpaH"
                     /locus_tag="VFF22_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HZX15510.1"
                     /translation="MLDASIIQQAAARLDAAERSREQVRQFSLDYPNITIEDAYAIQR
                     AWVDLKLKAGRKLVGHKIGLTSRAMQVSSNITEPDYGALLDDMLFEEGSDIPFERFIV
                     PRVEVELAFILGKPLRGPNCSIFDVLEATEWVVPALEIIDARIQQVDPDSKVTRKVFD
                     TISDNAANGGVVMGARPVRPNDIDLRRVPAILYRNGVIEESGVSAAVLNHPAKGVAWL
                     ANKLAPYDVTLQAGQIILSGSFTRPVAASPGDTFHVDYDQLGSIACRFV"
BASE COUNT         6423 a        10759 c         9697 g         6330 t
ORIGIN      
        1 ccgcagccca gctggcgccc acggcatcgc ctctgcctgc cgaggcaacc acagcgctag
       61 cactggtgcc ggatcgcgac tggcggctgg ccttgcgcac cgccaccacc accggcggcg
      121 cggcactggc ctgcgccttt ctggcccagg cctatccaag ccaggcgacc gagctggcga
      181 tactcggcgt gtgcatcttt tcgatgttgc tgggttccat ggcccaaccg cagaagcagg
      241 ttccgcatat gctattgggc gtgctggccg gcgtaacagg cgccacgctc taccgcttcg
      301 ccgtgcaacc ccacgtggac ggcatgctgg agttggtact gaccctgctg ccgttcctgc
      361 tggtgggcgg cattctgcgc gccagccgcc gttttgccgt ggcggcaatc gacgccaata
      421 tgtgctttat gctggccagc caggccggca tgcccacggc cagcagcggc gcaattatca
      481 gcagctcgct ggcgctgctg cttgcggcag gcctggtggg cagcggtttt ctgctgttcc
      541 cgagtaccgt gcagcgcgcg ctgcgcctga ccactcaggc cattctcggg gatattcgaa
      601 ccctgctacg cgacagcaat gcccgcagcc gggaacgctg gctggccctg gccggccgcc
      661 ggattctgcg cctgctggcg catatgggga ccgacacgca ctccggtact acgccgccag
      721 ccagcctgct gtcgttgctc aacctgggac agtcgatcat cgacctgcac cacctgggcc
      781 agcaacccgg ccaccgggcc atttatcagg aggccatcgc caggctggcg gacttcgagc
      841 gtcagccgca ggcgaccgcc gagtttctac tgcacctggc agaggacagc cgcgaccgct
      901 ggctgacggt gaccctgcag accctggccc atgccctgct ggccagccgc agcacgctac
      961 tggcgcaacg cccgagcatc agctcggcgt agccgtgagc tgctgcgtcc gcgcctgggc
     1021 atcgccaagc atggactgga tatcctgggt cgcctgacgt gtgctctgcg ccagcttctt
     1081 cacctcatcg gcgaccaccg agaagccgcg ccccacttcg ccggcccggg ccgcttcgat
     1141 ggccgcgttc aaggccagca ggttggtctg ttcggcgatc cccttgatca cgctgacaac
     1201 ccgggcaatc tgcccatagg tctgctgatt gtgctgcaac atctgctcat gggccgcctt
     1261 ggcattacgc tcatcggaga tgtcgcgcac cgcaccaatc acccggtaga gccgaccctg
     1321 ctcatcacgc actgcgcggc cacgctcccg gcaccagata taaccacggg tcttgtggcg
     1381 catgcggtat tcgaccacgt actcgccgct gcccgccggg tcgacgatct gtctttcaaa
     1441 caggttgaag atgccgttga gatcctctgg atgagtgatg ccgacctgac tgtcccagcc
     1501 atcgggcaac tcatccaggc gatagcccat caacacgcgg aactgattgg atatgcgcat
     1561 catgctgcct gggtcgtcga ccttgccatt ggccacgctg agatcccagt agccttcggt
     1621 cagggtggac tgggcaacct cccaaattct cgcctccagc tcgcaactgc tcagaacttg
     1681 ctgttgctcg tgcagctgag cctgaacagc ggccagggtc atctgttggt gcagcatctc
     1741 gtcgcgcagc tgctgctccc gggccaaggc ctgatcgcgc tcctcgcaca gccgctgcac
     1801 ctgctcatgc agccctgtcg tttcatccca ggtctgttgc ataagtccgg ccaactgctc
     1861 aaggcccttg gcgagctgcg gatagcgcgc gagcaaggag cttccaggca gcatcccccc
     1921 gcgaccggca ttcagattgg cgatctgctg gcacagcgcc tcggcaagat cacgcccgtt
     1981 gtcgcgcaag aacataagcg gctactccag ttcggttgat aaaggctgat gcaaacgctg
     2041 gtgctgtttg accgcctgcc actgttggtc gatcacccgc ttctgctccg ccttgagcgg
     2101 ctcgaggaaa tacagccggc ggcgctcgct cgcctgaggc acgagtgcgg gggcgctctg
     2161 cgccagcttg atcatctccg ccagatcgct gcgcagtggc gcatagaacc tggtcgccag
     2221 ggtattcaga cgggcattgc ccggctccag catgtaatcg ataaagcgat aggccagttc
     2281 gggctgctgg gcatctctgg ggatggccca gctgtcgatc gtcatcaacg ccccctcctc
     2341 tggcaccaga aactccaagg tgttgcgctg cgacgccgcc agcaaggcat gcccgctcca
     2401 ggccatggcc acgcacaggc gtccagcggc cagttcttcg atataggcct cgttgttcag
     2461 cgcatgcaaa gcagccgcct ggctggccag ttgtgcgccg ctatcggcca aacgcctggg
     2521 tgaggcctcg aacaagcgct tgccctgcag gttcatccac agcgagaagg tctcttccgg
     2581 ggcgtccagc cagcccatcc cgcacgcctg caggcgctgg gcatactggg catcgaagag
     2641 caggctccag ctcgccggcg cggcctcacc cagggcccgt tccacggcct cgcgctcgac
     2701 caccagacca accgtgctcc acagataagg caccacataa cgccccgcac cggaaaagcc
     2761 tgcgagcgag gccacgagct tgggatcaag attgccgtaa tggcgcagct tgccgacatc
     2821 cagcgcctgt agacggcccc cgccgatcag acgttgcaac tgaaaatgcg aaggggccac
     2881 caggtcgaaa cctgcgccag cgtccagcgc ctcgtccagt tcctgggcgg tggtgaaggt
     2941 ctggtaatcg accctgatac cggtatcgcg ctcaaagccg gcgagcactt gcgggttgat
     3001 gtaatccttc cagttgtaga tacgtaacgt ctgggcataa ccgctgacgc tgcagaatat
     3061 gagcacggcg catagcaagc ctctcatcat ccgggctcct tagtattgtt gtgccccata
     3121 gttaacgccc agcagcggca acgtcttgcc tgagcgtgcc gggtccttgt tccgcgctgc
     3181 cgcccaataa aaagcgcctc aaaagaggcg cttttcggtt ctgccggttc aggtcaaccc
     3241 agatcaggct cgaacagctt gcgcttcata tagagcttga cccagagcgc cgcgatcacg
     3301 ctgaccagca gcaggatgcc gccagtgatc ttgtagacca tcacggtaat ttccggcgac
     3361 ggcgagttgt tcagcgcgac ataaagcatg gcggcggtgc cgaggatctg cggcagcggg
     3421 tagaacggcg tcttgaacgg gcgcgcctga tgcggcagac ggcgacgcag caccagcaca
     3481 tcgacatgcg ccaccacata ggccagcagc cagctggtcg acgcggcgat caccagcggg
     3541 atcagcgagt cgatgtccat cgccagcagc gggatgatga tgatcgcagc cataaaaaga
     3601 atgcccaccc atggggtacc gaaacgcttg ctggtgatct tcatctgcgg gaaggcctgg
     3661 ccattctccg ccatgccctg gagcatgcgc ggtacggcgg ccagcacggt gttcacggta
     3721 ctgcaggtgg cggtcaccgc catcaccgcg gcgatgatca aaccggcctt gccgaacacc
     3781 gcattggcat aatccaggta cggcagtggc gaggtggtga gggtttcgat atccaggtaa
     3841 tgggtaacgc cgtagacgaa caacaggaaa atcccgagca tggcgcacag cgacagcatc
     3901 atcgcccgcg ggatattgcg ctgcggattc tcgacctcgt tgatcatcgg gcagataaat
     3961 tccacgccga tcatcagcca catggccatg gccaccaagc caataaagct gccgtcgagc
     4021 acaggatcga agctccagct caccgccgta ccggccagct cggcatgggg ctcaaccgcg
     4081 ccggtaaccg cacacaggcc gatcaagatc aacgctgtca caagaatgaa ggccagcagg
     4141 ttctgcactt tggcgaacac gtcggcaccg agaatgttgg tcacgacgaa gaagccgagg
     4201 atcagcagcg gcaacaccaa tggagggaac atcccaggca atagctcgcc gatgatgata
     4261 tcgaccagga acatttccac cggcagcgcc aacatggcca ccaccacgta accggcgaac
     4321 actgcgacga tcgccgggaa atggccgata gccttttggg tgtaggtcgc cagcgtacct
     4381 tcctggggga acatcagcga cagctcggca aagctcattg cattgaactg cgccagaata
     4441 aaagcgacaa agagcgcggc gataaagccc atgccgccaa taccgatgcc ttgagtcgcg
     4501 gtgatcatcg agccttggac aatcaccaga ccgacacaga tggccatcat ggtgataaag
     4561 cccagtttct tgtgctgcat agaggtagcc ctccccgccg attttgctat tgaggtaaga
     4621 cctggggcgt tggtatcagt cattttcttt attctcccgc tcgggccatt gccagcccaa
     4681 aggtgcccat ggatgcgacg gcggtttcgc gccgtgtcgt gtatcactgt gctactgcat
     4741 ccaggagaat tcttgtccca ggctgccgcg cccttgtccc ggtgtgtcag cggggctgct
     4801 ggcaggccaa taagacgata ggatttgctc caattgcttc ttcactaagg gtatagcagc
     4861 catgcaaaac gtactgatca ccggtgcctc ccgcggcctg ggcctcggtc tgacccgggc
     4921 ctacctggcg cgcggtgcca gggtctatgc cgtgatacgc cgccagccca gcggcgaact
     4981 gctcgccctg agccaaggtc acgccgcaac attgcgcctg atcctctgcg acctcgacga
     5041 cgatcaggcc gccgcccgca tcggcgaagc cctgcaaggc acccgtctgg atcgggcact
     5101 attcaatgcc ggcatctacg ggcctgccca tcaggatcca tgccaggtca gccacgagga
     5161 gattgcccag ctatttctga gcaacgccat cgcccccgta cgcctggcgc aggcactgct
     5221 gccgagcatc cgtgaagacg gcgtactggc ctttatcagc tcacagatgg ccagtgtcga
     5281 actggcccgc gccacagaca tgccactgta cggtgccagc aaggcggcgc tgaacagcct
     5341 gctgcgcagc tggagccagg tgccggactt gtcaccggtt acgttgctgg cgctgcaccc
     5401 cggctgggtg cgtaccgaac tcggcggacc cgatgcaccg ctcagcgttg aagagagtgc
     5461 tcgcgggctg atcgaggtga tagaggcaca ggccggccag ccgggttgtc gttttctcga
     5521 ccatcagggc cacgtgctgc cctggtaggc agcgacacca gcgctgtacc gtttgagcgc
     5581 tagttcgcgg ctaaagcccc tcccgcaggt gtgcacaagc gacaacgggc tcccggtctg
     5641 aaaccgggag cccgttgctc tagtgtgctg cctcgcaata atcattgctc agctgctacg
     5701 gccggcctgg tagggtgcgc cgtgcgcacc actgacaacc gggtgtctag ggtgcaaaca
     5761 acgctttcag ccgatggcga tcaccgcctt gagcatggct ttgaaggtgt cgatctgctc
     5821 ttcgctgaag gcaccgaaca ccttgtcctg ctgctcgccg gcaatacgcc agagtgcttc
     5881 ggcctggtcg acgccggccg gggtgagcac atagcgatcg tcggcatcgg ccaccagccc
     5941 cttgcgcttg aggatatcca ccgcctgttc gatctcgcgc accggcatcg ccacttcccg
     6001 cagcaggtcg cacaggctca ggccggcatc gttctccagc accatcagca tccgcgcctc
     6061 gctggtgcgc aggccggtgg acagctgccg cggctggtaa ttgctctggt agctgcgcac
     6121 cgcctgggtc atcaggtagt aaaggttgtg gctcagtcgc ccctgaaagg cgctactggg
     6181 ctgctgcccc tcggtctttt cggtcatgcg ggtatgcggc agcaccatgg agtaagcccc
     6241 ctggtgatag agcagcggcg agcggccgaa gtcgtcgaag gccagcacct tgccgaccag
     6301 gatccaatga tcgccaccgt cgatctgttg atgcttctcg cactgaaaac gcgccgcgca
     6361 gtcgggaagc agaggcgcgc cgccctcgcc aagctcatgg gtcacaccgg caaacttgtc
     6421 gtcactcggc cgggcgaact ggttggacag gtcgatctgg tcggcggaaa ggatgttcac
     6481 cgcaaaatgt gcggcctgct cgaacacctc gtagctgccg gagcgcttgt cgatgctcca
     6541 gagcaccagc ggcggatcca gcgacaccga gttaaaactg ttggcagtaa cgccgacctt
     6601 ttgcccgtcg gcagtggccg cggtgacgat ggtcacgcca gtggcaaaat tgcccagggc
     6661 gcggcgaaag gcacgcgggt caagagcagc agtagtgctt gtcatgggat tctccctggc
     6721 taccgtcgag gttcgcggca gcgctgttat tcggctgtcg gggccgcgaa cggccccacg
     6781 caaggctggc tgggtcagac catgctcggg tccggctcca ggcccatcag ctcacggcca
     6841 aggatctgcg cgcagacgtc gtagtcggtg taggcatggg cagcggtcat atgcgagtcg
     6901 cggaacagcc gctgcatctc gttgctctcg aaccaactgg tgccgccggc ggcctcgaac
     6961 aggcgatcga ccgcctggat gcacatcttc accgcgtagg cctgattggt gcgccagaac
     7021 gccagggtct cgcggctcgg gtactggtga cgctcgccgt gttcggcgtg ctcctgccag
     7081 gtcttctcga ggaaggcacg ggcggcagcg acctgatggg tcgactcggc caggcgcatc
     7141 agcgccggcg tggcggtgcc gacgttgacc ccggtatagg cacgcacgcg gttcttggtc
     7201 ttctcccgat acgcctcaag catgcgctcg gcgataccca ggccgatggc ggcgaaacca
     7261 ctggcgaagt acggccggta aggcgtgtag aaaatcttgc tgtccgggta cagaccgaag
     7321 cccgcggact tgccctccat catgtccttg gccttctgga tacggtggtt gggcacaaag
     7381 acatccttga tgatcagggt cttggtgccg ctgcccttca tcccggcggc gaaccagtcg
     7441 tcacggatct cgtagtcgct gcgcggcagc acggcaaagc aatagtcctg accgccttcg
     7501 tcgttctgcc ggcgacagcc gacgatggcc cactcggcat gatcgctgcc gctgctccag
     7561 cccatctcgc cattgaagat gaccccgccc tgcacctcct cgaccttgcc gaacggcgcg
     7621 atgctgctgc tggcagtggc gtccggattg tcgccccaga cctcgtcctg cagttgcttg
     7681 gcgaacatcg ccagttgatg actgtgggtg cacagcaggc tgaaggccca ggcggtgctg
     7741 gcacaggcac ccgccagggc aaccacgcaa tcggcaaact gcggcaagga gatttccatg
     7801 ccgccatagg gcttgggctg aaaggcacgg tgcatgccga tacccttgag cagggcgata
     7861 ttctcgtccg gtgccttgcg gtctttttcg gcctgagcgg cattggcggc aatcgccggc
     7921 aatatggctt tcaggtcttc gagaagcggg tttggctttt tcatctggta ggggccctgc
     7981 ttattgttag aaggtggtcg cgtcgtcagt cgtgccggct atacgcctga ccaggctttg
     8041 cggcctcgga atccagcacc cgtagcgctg tatatccctg tatgcaggtt cattatgctg
     8101 agagctaggc tggctaaaaa tgcaccttcc agacactaaa ttgcactttt catggcttta
     8161 agggcaggat tgcccgccag aaggttaacc tgttaatgaa accccgatag gccttaccat
     8221 agcgccccac accaccctct cccagatcgc catgagccac ctgcccagca ccctgcgcgc
     8281 cttgcgtcat cgcaacttcc gcctgtattt cattggccat gccgtctcta ccctgggcac
     8341 ctggatccag caggtggccc tggcctggct ggtctaccgg ctgaccggtt cggtggcgct
     8401 gctcggcttg accacctgca tgagcttgct gccgcagttg ctggtcggcc cgttcgccgg
     8461 agcctggatt gatcgccacg acaagcgccg cctgctgatc ctggtggaaa gcctgctcgg
     8521 ggtgcaggcc ctggcgttgg cgctgctcac cgccggcgac tgggtgacgc cgacactgtt
     8581 ggtcgccatg gccactctga tgggtgtgct caacgccctc gacacgccgc tgcgccagtc
     8641 gctgctgagc cagttcgtcg acgaccgtgc cgacctgccc aacgccctcg ccctgaatgc
     8701 catgctgttc accagctcac gctttatcgg cccgcccctg gccggtctgc tcctcgcggt
     8761 attcagcgag gcgctgtgct tcgccctcaa cggcatttcc tacctggccc tggtcatcgg
     8821 cctgctgtgt atccgcatag cgccaaccgc cagggcggct ggctcctatc gcaatgtgct
     8881 gagtgagggc ctgcgctatg ccctgggtaa ccgccaagtg cgcgccatga tgctcagcgt
     8941 gctggcgatc aacgtcagcg cctccagcta tgtggtgctg ctgccagtgt tcgccaagga
     9001 tatcttcgcc ggcggcgcaa ccaccctggg ctggctgtgg ggcgcagccg ggcttggctc
     9061 gctgctgtcg acggtatttc tcgcctcgcg cctggcggtg gacagcctgc ccaggctgat
     9121 cctcgctgcc agctgcgcca ccgccctggc cctgctggtg ttcgcctgga gcgacctgct
     9181 ggcactgtcg ctggcggcca tggcggtgat cggcttcggc atcacggtga gcaacgtggg
     9241 caccaatatc gtcctgcaga gcaacgcccc cgaagccctg cgcggacgca tagtctcgct
     9301 ctacacctca acccgcttcg gcttcgacgc cctaggcggt ctgctcgccg ggctgctggc
     9361 cggttacctc ggcgcaccgc cggtgatggc gggtgcgggg gtcgcgttgc tgttgtactg
     9421 catctgcgtc gaactgcagc gccggcgcaa ctagtgccgt gccgaccctg aaaatcgctt
     9481 cgatgccagg ctgctgtgcc gctaggtgaa catcatccac atggccggcg tgcccggctt
     9541 attcacttgg gctgatgtcc ctctgactgc tccagaaggc ccaggcagac acgatgaccc
     9601 tgggcatgtg agtgctggcg gtggtggttt gtgtgtatag gttgttagca gaattgaccg
     9661 cgataggggc gactttcggc caaaagcggc cgttcaccag tgattatttc ggagcggcac
     9721 tggttgccca cagctaggcc tgccccttaa agctgtaagt ccgtatatac gtatttgcat
     9781 cagccaacac attcagtcag ctgctgtaca caatcctcaa tcgacaggct cgaagtcatc
     9841 aaggtcaagg ttggttgctg cggtggctca tacggggagg tgatacccgt gaagtctgca
     9901 atctctcccg ccctggcccg ccggtatagc ccttttacat cgcgcgcttc gcacacttgc
     9961 agggagcaat tgagataaac ctcggagaaa tcattcgcgc cgacaatatc gcgcaccatt
    10021 gcacgatcac ggctggtggg agagataaaa gccgcgatgc aaatcagccc ggattcggca
    10081 aacaacttgg caacctcggc agcacgccgc aggttttcat gtcgattatc tggagtaaaa
    10141 cccagatcag cgcacaagcc acctcgcagg ctatcgccgt ctagcacgac gcagcgccgt
    10201 tgcagcttat acagccgtgc ttccagggcg aaagccagcg tggactttcc cgaaccggag
    10261 agccctgtca gccacagcac ctggccgcgc tgggcgttta gcgactcgcg ttgctcacga
    10321 ctgacctgcc ccttgtggcg gtagtcgggt ttgccactac cgctcaccga aggactcact
    10381 cagggttttg cgtaggggct tgagcaaata gtccatcacg gtacgttcgc cggtgcggat
    10441 atcgacctgc gcggtcatgc ctggcagcac atccagcacc ctgccagtcg ttgaggtcac
    10501 ggggtagctg gctggtttga tccgcacccg gtaatagatg tccatgccgc gactggtttc
    10561 ttccttcagg ctatcggcac tgacaaacgc aacctcacct ttaacgccac caaaaatggt
    10621 gtagtcaaag gtatcgaaac gcacagtcgc ctccaggccc ggtaccacac gagcaatatc
    10681 ggcggtgctc accttggcct caatcatcaa gtcatcgtcg accgggatga tttgcatcag
    10741 ctcttcaccc gccctgagca ccccacccac cgtggtcacc cggatgtttt tgacgatgcc
    10801 aggcaccatc gcagtgaaca cactgtcctg ttgttcctgc ttgcgcaggg tcagggtttg
    10861 cccgctttgg gagatgtcat cttcggcctt ggccagctct agtcgggcgt cttccagaaa
    10921 cttgttgcgt cggttcacca gacgcgcctc ggcctcattg agcccgcgtt gcacgcgcaa
    10981 cagctcagag ccactggcat caccgttcgc gtaaagctgc tcaaccagtc gtaactcctt
    11041 ctgcgccagg gaaaccgcca cgcgcatggt acgcagctct tcctcaaggc ccagttggcg
    11101 ctgcttgaat aatgcctctt cgacacgtgc tgtttccggg gaacgctcaa gaagatctgc
    11161 ctgaaagctg agccgtcgct caccaataac ctcagcccgc agccgggttg ccctagcctg
    11221 caaaccaaac aaccgggctt ctacttcacc gacggacgcc ccaagccggg tttgctccaa
    11281 acgcgcgagc aactgaccgg gggccacccg gtcgccctcc ttgaccagca gttgctccag
    11341 tacaccacca tcgaccgact ggataatctg cacacggctg ctggcaatca cctcacccgt
    11401 agcacgcgcc acttcatcaa tacgaaacaa cgatgcccag atagcaaagg tgacaacggc
    11461 gatcagtaca gccatcagta accagccctg gcggccacgg tgtgagttct gcagcttgct
    11521 gacacgcgct tgattacgtg cgtcgtcgtc ctgagagaaa cgatcagacc acatcgagcg
    11581 tccctccttt acccatacgc gatgaggcaa ctggacgggc gagcatctgt gccagcacct
    11641 tgtctggcgt gccgtctttt gtcacctgcc cctgctgcat aacgatcacc cgcgttgcca
    11701 gacgcgttgc ggccatcggg cgatgggtcg caacaatcaa aatatcttca ggccggacgg
    11761 cctcttgcag agccgcccaa acacgctgtt cggattcacc atcaagcgca gcagtaggct
    11821 catccagcag ccatatacgt ggctggttga tcaaaacccg agccagcgcc accagctgcc
    11881 tctgaccacc cgacaatccc tcccctcctt cgctgatcga caaatccata ccctgggggc
    11941 tggatgccgc aatcgcatcg atgcccagct ctcgggtgat ctgcaacaag cgtgagtcgc
    12001 tgttcgcgcc ggtcaatgcc aggttgctgc gcaagctgcc cttgaacagc tgaacggttt
    12061 gcggcagata gcccacctgg ctagccacca gttgtggatc gatctcccac agatcggcat
    12121 cacccaggcg cacacggccc tcactggggc gataaagccc tgccaagact ttcagcagag
    12181 tcgatttacc acagcccacc ggccccacca gcaaaacacg ctcgccagat gtgaaacgca
    12241 gcccctcgat attgacctgg caaacgggcg actcaggata ggcaaaacgc acacggtcca
    12301 gcgctatttc attgggcaac tcgtcgggta acagcaggtg ctggtccggc cggcgatcac
    12361 gctccagaga caacacctga tgcaccatct gcagcgcctg ccgaacgtgc tgccactggg
    12421 cgaggtactg aacactttgc gcaattggcg caatcactcg accacccaga atgctgcacg
    12481 cgatcagccc gcccatcgtc agcaggcccg cctcgatctg ccataccccc acgaccacag
    12541 cagcaacata ggccaaggtc gacaggctac tggtgctcac actggaaaaa ctgctgatcg
    12601 cgcgttgctg aatgctgtag ccatcgatgc tggcggtgat gctttgccac tcctgagcaa
    12661 agcgccagcc ggcgttattg gcacgaatgg actcggcgcc acggatactg tcgaccaaca
    12721 aaccctgccg ttcattgctg cgtagcattt gctgcttgag caatgcgcgc aaacgccatt
    12781 gcgtgacata gcccaataac agagccacgg gcagtaacac cgcatacacc cagccgaccg
    12841 cgccgccaat gatggcgatg aacgccagaa acagcagcgc aaagggcaaa tccaccaacg
    12901 cgaataccac gcccgagctg aagaactgac gcacggtatc caacccaccc acctgcgcgg
    12961 ccagcgtgcc taagctgcgc ggctgctgat ccagacgcaa atgcagcaaa tgctcgaaaa
    13021 cctgctgcga aacccgcttg tccaccgcac aggacaggct gtccaaaatc cgcgcacggg
    13081 tggttttcag cagccagtcg agcaggatga tcatcgccat ccctgtgacc agagtggtga
    13141 gggtcgcgta tgccagcgtg ggcaccacac ggtcatagac ctgcatggca aaaattgacg
    13201 taccaaccgc cagtagattc accagcaccg tggcaaccat gaccttccac agccagccgc
    13261 gctcacgaaa taactcacgc cataccaaac gagtcgccag gttgcccgag aacgctcggc
    13321 ccgcatcggc gttccagctt gtcggggcac gcagccagac caccagggca tcctgcagct
    13381 ccgtgtcatg gacttcacgc cgctcgcctg cggcgttgat caacgtcaca gcatcggtcg
    13441 cctcacgtac cgctaaaaac cagccgtcat ccgtgcacac cagggccggt aagcggcgca
    13501 gatccagacg gcgccaggcc aactgcaccg gcacaaggtt tttcaggcct gcctgaacca
    13561 gcacctgcct cagccattcg gctggtttga ccgcagcaaa cgtatggtcc acgtgcgaac
    13621 aggcctgctg taacgcagcg ccatcacagg gaacctgcaa agacgccaat aactgcttaa
    13681 ccagcgcaaa cgcaataccc ggttgctccg atgcgccgtt ttgcccatcc atgtgcttac
    13741 agctcctcgg tatttaaggc gaccagcgca tccagcccgc ctgtcagcgc cgccagctgc
    13801 agggaataga tcacccaatc gctttgtgct tgcaccagct gcaattgctg ctcgctgagt
    13861 tcgcgctgga tattcagcac atccagccag ctcttggtcc cagccgcgta ttgacgctga
    13921 taagacccca acacagcctc caactcggtc agcgactcgc cctgcacctg aatcaactca
    13981 gcctgcaact cacgattgcg ctgcaaccgc cgcacgctgc gttcaagctc gttgacggca
    14041 tacgtcaggt cgtcttcggc cgcgctctgt tgggcgaccg ctgcgcctgt tcggccacgg
    14101 gtcgcgaaac ccatgccttc cagcgtaccc tcaaacacca ggctgacacg ggtgtcatcg
    14161 cggtatgacg gctggtcata atcctgttcg gcttgcagat aaaccgttgg catagaggcg
    14221 gttttggctt ggtccacgcc ggccttggcc cgttcgatct cctgcttctt gaagcgcacc
    14281 tcagcactgc cctgcagggc tgcctggcgc aacgcttcgc cctcggtgac gtgcaacaaa
    14341 ccagaggcaa ccggtagttg agccggcaag cgcacctggg tcagggccag caagtcgctc
    14401 tcggcaattt ccagctcgcc tgcgtaacgc tcaacccggg ccttggattg cgacaggcgc
    14461 gtggccgcta gacgcacatc cgcagtcgac gccaattggc cctgctcacg acggctgatt
    14521 tgatcaaaca gtgactggtg ggcggccagg ctggcctcga ccacttgtaa acgctggcgg
    14581 ctgcccacca cgctggcgta ggcaacagcc gtttgctcca gcagttggcg ctgcacccgc
    14641 agcaggtcga cgcgctcgac atcggtgtcg gcatcagcaa acgcaatgct gttatcgatc
    14701 cggccaaagg cccacagtgg ctgacgcgca cgtagggtga ccggggtgga aaggtcttca
    14761 ccggtggtgg cgtctcggtt gtcccccgaa tactgctgag cctgcgccgt caggctgggg
    14821 tagcgctggg cacgcgcagc atcggccgag tattcacggg ctttgatctg cgactgcttg
    14881 ccactaatcg ccggatgcag ggtcagtgcg gcctgcaagg cggcctgcaa accatcggcc
    14941 tgctgtgcat tgacgggcaa cgccaacaca cctaaacaca gggccagcgc gctacgccgt
    15001 cccaggcggg gcaaggaaat caccatgcaa actctccatc catttcttgt tcggggtccg
    15061 gcacgctcgc gtgcggtttt ttaggtttca aggcagcggc tttttccggc tcaccggata
    15121 attcatacgc ccgcgccagg tcgtcacgcc atagaccatt ccacgggcac tgctcgtggg
    15181 ctttgagcat caaggcgatg ccctcactca catggccctt gcgcaggtca atcaggccca
    15241 gcataaaagt ggcggccgca tgctccggta ggtgatgcaa cagctgtttg aagtgatact
    15301 cagcgtcatc cagcttttct tcacggtaaa acgccacgcc ggtttccagc aggccgggta
    15361 ccggtagcgc gaccatctcg gtgatcggtt gccattcgat gcgcgcgttg gtaccgcgta
    15421 tcaggggcgc aaggtaggct tcataacgcg cccatttagc catggaggtg gtgtaaagcg
    15481 gctggcgcac ctgccacacg ctggcggttt tcaccggccg ttgcagctcg ctgaaattga
    15541 gcacctgcgg ctcccacccg acaccgacat aatccagcat gcgccgcgtc tcgcgctctg
    15601 ggtcggccaa tacatcctcg tagcgcacct ccagaatgtc cccagggaac acctggttcc
    15661 agtggtgcat cagcaggtta tggtcggcca gttgctcgcc aatccagccc aggtcatagg
    15721 cgaagcccat gccaccgtgc ttggcggcgt aatcggtaaa gtagttggag atcgcgatat
    15781 cacgcgggtc gcggcgcacc gagatgatcc gcgcgttggg aaacagcaac ttgatcaggc
    15841 cgatgttctc gaagttatgc ggcagcttgt ccaccacatg cagcgcctcg ggggcgtact
    15901 cctgcagctc cttgagcaca ttggcggaga tgcccgcaac caccttggcg ctgaggtcgt
    15961 ccacacaatc agggtaatgc cggcccgagc ctacgtggcg ctcccagcgc tccagcccgg
    16021 cgatcacact gggcatcacg cccagctcgc ccgcgccatg gatctggcta tggccggcaa
    16081 gcatctgctc gatcagcgtg gtgcccgagc gcggcatgcc cagcacgaac acgggcaggg
    16141 tcgaattgtt gcccacgcct gggcggtgct cgtacaagtc acgattgaac gcatggcgaa
    16201 tacgcgcaca acgctgacgg tgtgctgctg ggtcgtagct cagcaactta cggctggcgc
    16261 tgttggcttc atcggctaac gcaaaagcct tggcgtaatc cttacgcttt tccctggcgg
    16321 cggctaattg cagcagtaag ccgggctgca ccgggccttc taggccaggt cggcgggcca
    16381 ctgcttccaa gcgttgtaac gtggcgtcgt catccgggaa acgccgcgca ttgatcagcg
    16441 cggcatggcc acgtaccggg tccagggcct taacctgctc gaacaattcg atggcgtcgt
    16501 caatgcgccc aagctgcata tacaggtggc ccaggccctg caaagcaatt agcaactgcg
    16561 ggtgagcatc cagaacctcg cggtagcgcg cttcagcctc gacatactgc tgttcttgcg
    16621 tttcaactcc ggccagcgcc acctgtgtct gggctaacca atgcgcaatc tgcgcagggc
    16681 tgacgtcctt gctcggctgc agcgccaggg cttcttcaat agcctgctga gccatttcac
    16741 gggccagtgc gggatagttt tgggtggtca tgctggccaa cttcacgcgc aaggccagcg
    16801 tgggcttttg ctcttggctg gtcatgcgaa gcgcccggcg cagcagcgct atagcctgag
    16861 gcatgcgccc ttgcacgatg gccaactgcg ccagctgctg tagcagccct tgctgcgccg
    16921 ggtaatgccg cagaccacgg cgcagtgcgt cctcggcctg agcatggcga ccgcgctccg
    16981 tcaggcgcag ggcgtagccc cccaacaagt cgcaccacgg ctcttcttcg agcacgacct
    17041 gctgcgcgtc cagccaggca tcagcagccg caaagccctc attggccaac cacaggtcca
    17101 ggcgcaaacc gcgcaattgc tgggtgtgcc aagccgcctg cgcctccggc caatgcgcct
    17161 gttcaccggc ccagaacgcc tgcgcctccg cccaggtgtg ctcagcgcgc gccaggtctg
    17221 cgctttgcag gtataggtgc agcagatgct caaacaccag aatacgcggc tgcaaagcaa
    17281 ggctttgctc gtacaactgc cgggctacct caggctcctg gcgttgcaaa tggcaggctg
    17341 ccaggcgcgc gagcaatagc gaatgttcag gggcgtcgac aagggcatcg gtcaacagca
    17401 cctgggcggc atcggtatcg cccagcagca aatgcaccgc cgcgagatcg acccgcgcct
    17461 ccagatcgag cgccgatacg tccaacacag acagtgcctg ccgggccgcc tgggcatcgc
    17521 caagatgcgc acaggccaac gtccacagca cgtgttcggc tggatgttgc ggtgaggaat
    17581 cgctacaagc ctcaaacaac aactcaaatt gccgttgttc gaccaggcgt cgcagcagag
    17641 gaagagaact cattgatgta acgcggccca tgggacaatt cgaccctccg tgaggagggc
    17701 cgaggatggc gaaaaggtga tgaaacgtcc tagaccagac cctgggaggt cagaatgccg
    17761 gcaggtatct gcgtcaggta gacgtttgct cccacggccc ccaagtgctc ggtattgggg
    17821 tttgcagccc aacttccgcc ccaaatatcc ataacagcag agatgccgtt ggagcgttta
    17881 agctgaccat ctaaacccag ttcgacagca taccccgaac ttgcccagat caaagtattc
    17941 ggattaccta gcaggtaagt taccccgaag ccagctgtgc ctttgactgg agtacccgta
    18001 cctaccaaca ctatgcggtc atcaacacct aagttattcg caccagacca cgtatcccag
    18061 gtacctggcc cggaacccgc agtgctaagg acgtggctgt tactgtcaac acggataaag
    18121 atggtgtagg aggtattggc atcaaaggtc cggccgctat ggtcagaact cacgccctgc
    18181 accaagtcga acacaacgat gctgctatct acggtctcag tgtcgaagtt cagcgtcgta
    18241 tcatcgttga tgccggcgaa agcattaccc gcttggtcgg tcagcacacc agcggccatc
    18301 tgcacgttat aggtactgcc cctctcaaga tctagcgtag ggttgatagt cactatgctg
    18361 ccgctgatgc tgacctgagt tgcatcagag atcgagatgg tgcgggtatc agtgccattg
    18421 ctgatgacaa tattgccact accagccaac acggtttcat tgaaggtcaa ggtgatgttg
    18481 ttgccgatgg cgatgtccgt ttcgttatca gttggggcgc tggagctaag cgtgggtgcg
    18541 gcagcgtcta ccagatagct ggcgtggtcg gccaccagcg cgtgagtcag caccgcgtcg
    18601 ttcccagtcg cgtcgcgcag cgtaccgccg ttggcctcca gcgcgttggc tgctatcgag
    18661 ataccgttgg ggtcggtctg gctcgccagg atggtgtact ggaagactag cgacgcagta
    18721 cccgtgcccg agacgtaatc ggcgtagacc gtactgctgc caattgtcag cgccaggcgt
    18781 ggcgtacctc cggcggtatt taccagcgtc gcctcattca tccccacggt tacgctgact
    18841 acatccccgg cattcaaggt gttgttctgc acgcccgtgg cgctggtgat ggcaacgctg
    18901 ctgaccgcag gcccagtgtc gtccacatca ttgatgctca gtgaaacggc ctgttcagtg
    18961 gtattacccg ctgcatcggt ggccgcaacg gtgaagctat agctagcctt ggtctcgtag
    19021 tcggggttgc ccgtaagcgt gaccgcgcca ctgacaccgt cgatgctgaa ggctgcatgg
    19081 tcgcccacaa ctttgaggct gtaggtgacg gtattggcgt cggtagccgc tgcggtatag
    19141 accgtttgtc cggcaccgct gttttcactg atggcagccg ccacggcgcc tgaagtcacg
    19201 ctgggtgccg tggtatcgac ctgatagctt gcattgtcgg caacagcacc atgggccagc
    19261 accgcgtcgt tacctgccgc atccttgagc gtgccgccat taagttcaag cgcattgagc
    19321 ggtatcgcaa taccgttgac atcggtctgg cttgcctgga tcacgtactg aaacaccagc
    19381 gcgctgctgc cgctgcccga gacataggtg gcgtagaccg tgctaccgcc aatatccaag
    19441 gctacgcgtg gcgtgccggt aacgatggtg ttctcgtcca tggtcacggt gacgctgacc
    19501 acgtcgcccg cattgagcag gctaccctgg gcgccagtgg cgccgctaat agccacgctg
    19561 ctgaccgtcg gcgcgacctc atccaggtca ttgaccgcca gcgtcaccgc ctgctcgctg
    19621 gcgttaccag cggcatcggt ggctacaacc gtaaagccat agctgggctt ggtttcgtaa
    19681 tccgggttac ccgtcaacgt cacagcacca gtgccggtat caatgctaaa cagtgccgca
    19741 tcagcatcgg ctttgaggct gtaggtgacg gtacctgcat cagtcgccgc tgcggtatag
    19801 acggcttgcc ctgcgccgct gttttcgtcg atggccgtgg ccgtaacgcc cgagttaaag
    19861 ctaggtgcgg ttgtatccac cagatagctg gcgttatcgg tcactgcacc atgatccaga
    19921 tcggccgcat tgcccgccgc atcgcgcagg gtgccaccat tagcctgcag ggcattggca
    19981 tccagactga tgccattgcc gtcggtctga cctacctgga ttgtgtattc gaaaaccagt
    20041 gccgtgctgc cactgcccga gacgtaatcg gcatacaccg tgctgccacc gacattcaag
    20101 gccacacgcg gcgtaccgcc cgtggtgtcg accagggtgg cctcacccat ggtcacagta
    20161 acgctgacca cgtcaccggc attcaggctg ttgttctgcg cgccagttgc agcgctgatg
    20221 gcgatactgc taacggtcgg cgcgacttca tccaccaagt cgttgatcgc aagggtaaca
    20281 gcctgctctt ggaagttgcc cgccgtatcg gtggcgacca cggtgaagct gtaactcgcc
    20341 ttggtttcgt agtctgggct atctgtaagg gtgaccgtcc ccgtcaccgc gttaatgctg
    20401 aacagtgctg aatcaccacc gggtttcagg ctatagctgg tggcgccgct cacaacgtca
    20461 tcgttgtcgg tgctggtgac ggtatacacc acttgcccgg caccgctgtt ttcgttgatg
    20521 gcagtggcag tagcggccga ggtaatagac ggcgcggtgt catccagatt gttgatatcc
    20581 agcgtcaccg tttgctcgat ggcattgccg gcagcatcgg tggcgaccac ggtaaaggcg
    20641 tagttggttt tgctttcgta gttggggttg ccagtcaggg tgactgcgcc ggtgctggcg
    20701 ttgatgctca gcagcgcatg atcaccatcg gctttaaggc tataagtcac gccgcccgca
    20761 tcggtcgctg ctgcggtata gacgatctgc cctgtgccac tgttttcgtc gatagctgtg
    20821 gccgtagcgc ccgagctaat gacaggggca gtggtatcca ccaggtagct ggcattgtcg
    20881 gccactgcac catgatccag atcagccgca ttaccagctg catcgcgcag ggtgccacca
    20941 ccggcctgca gtgcgttggt cgcaatgctt atgccgttgg cgtcagtctg accggcctcg
    21001 attgtgtatt ggaacaccag cgcagtgctg ccgctgcccg acacgtagtc ggcgtacaca
    21061 gtgctgccgc cgacactcaa ggcgacacgc ggcgtgccgc cggcggtgtt gaccaaagtg
    21121 gcctcatcca tggtcaccgt gacactgacc acgtcaccgg cattcaggct gttgttctgc
    21181 gcgccagttg cagcgctgat ggcgatactg ctaacggtcg gcgcgacttc atccaccaag
    21241 tcgttgatcg caaggctgac aacctgctct tggaagttgc ccgccacatc ggtggcgacc
    21301 acggtgaagc tgtagcttgc cttggtttcg tagtctgggc tatctgtaag ggttaccgca
    21361 cccgtgacgg cattgatgct gaacagtgct gaatcgccac cgggcttcag gctatagctg
    21421 gtggcgccgc tcacaacgtc gtcggtgtca gtgctggtga cggtgtaaac gacttggcct
    21481 gcgccgctgt tctcgctgat agccgttgcg gtggccgctg aggtaatagt cggggcggtg
    21541 tcatccaggt tattgatacc cagcgtcacg gcctgctcgc tggaattacc ggcggcatcg
    21601 gtggcgacca cggtgaaggc atagccggtt ttgctttcgt aattcgggtt accgatcagg
    21661 gtcactgcgc cagtgccgac gtcaatggta aacagtgcag catcaccacc cgccttgagg
    21721 ctataggtca agctgcctgc atcggtcgct gcggcggtgt agacggtttg tccggcgccg
    21781 ctgttttcat cgatggccgt ggccgtagcg cccgagctaa tggcaggggc ggttgtatct
    21841 accaggtagc tggcgttatc ggccacggcg ccgtgagcca aattggctgc attacccgcc
    21901 gcatcgcgca gggtgccacc atttgcttgc agggcattgg catcgaggct gatgccgtta
    21961 gcatcagtct gtccggcctg gaggctgtat tggaaaacca gcgcggtgct gccgctgccc
    22021 gatacgtagt cggcgtacac agtgctgccg ccgatattca gggccacacg cggcgtaccg
    22081 cccgtgatgt cgaccaggct tgcttcatcc atattcaccg taacgctgac cacatcgcct
    22141 gcatttaggg tgctgttctg cgcgccgctg gcactggtga tcgcaatgct gctgactgct
    22201 ggtgcggttt catccaccaa gtcgttgata gccagggtga cggtttgctc actggcgttg
    22261 tttgccgcat cggtggccac cacggtgaag ctgtagctcg ccttggtttc gtagtctggg
    22321 ctgccgataa gggtgaccgc accggtggca gtgttgatgc tgaacagtgc cgcatcgccc
    22381 ccggctttca ggctatagct ggtggcgccg ctcacaacgt cggcgctatc ggtgctggtg
    22441 acggtgtaca ccacctgtcc ggcactgctg ttttcgttga taggacttgc ggtggcagct
    22501 gaggtaatag tcggtgcggt gtcatccagg ttattgatgc ccagggtgac aacttgctcg
    22561 ctggcgttgc ctgcgacgtc agtggcaaca acggtgaagt tgtagctagg tttggtttcg
    22621 aagtcggggt ttccggtcag agtcactgcg ccggtgctgg cgttaatgct gaacagtgca
    22681 gcatcatcac ctgccttgag gctgtagctg acaacgctgc cagcatcgct cgcggcggct
    22741 gtgtaaacga tttgccctgc actgctgttc tcgttgatgg ccgtggctgt agcgcccgag
    22801 gtgaagtccg gcgcataggt atcaaccacc ccagcaactg cgccggtcag gtcttgcccg
    22861 gtctgcgttg acgtaaactg gccgccttgg accacgatgc cttgcgcact ttccgtcagc
    22921 gttgcagtgc catcgctgac atccaaacct gccagcaagt cattaattgt ctgctggcta
    22981 tcgccaccat tttgggcatc cgcaccggat aaagctgcta gcactgcgcc ataaatctca
    23041 cccgaggtga gtccatcatt gctctcgaaa ctccccccat tggtcggtac aactgcagtg
    23101 ccgtgcaaat tgggcaaccc aaagagctcg gcgatagcgt cattggtact gtcgaccagc
    23161 gcagcggtag gtaaccctcc agcggctgca tcttgcacct tgtgataggc caaggtactc
    23221 aggacgttga tattgacggt gatcgtggag ttgggttgtg acaccacttc catcgcgaat
    23281 agttcggcgt tgaggtcttt accagcctcg gtagcttcat ccaggtagtc cgcgttcaaa
    23341 cccgcgttga cgactcgcgc aatgaccacg ccaagatagc ttccaaccgg aatggaaaag
    23401 gtgccgtccg cattgatctg ggcctcgcct aaaagggtta taccgtcagc ctggtacacc
    23461 tctacgcgca gatcattacc cgctaccacc ggaccgccaa cgatggtccc attaaccgta
    23521 ttgagcacgc ttgaaggcgc ctcgccgcca ccgctactac cgcctccgcc gcccccacct
    23581 gcggccagcc ccaggccaac aaggccgaga ccgcccagca caagattcgt cggcgtgacg
    23641 agatcgctat cagaaccgcc ccgccaaatg atttcatcac catttgcggc agtatcaatt
    23701 accggcgttt cactggtaac agaagtgccg accagcgcgg aaccgtctaa ctcagcttgc
    23761 ggaataaaag cagtttctgg ctcatcataa aactcagtga tttcgaccaa aaacccgtcg
    23821 tcctctgact ctacaatcag cgaactgcct ttgcgcttga cttgcaagcc ctctggcgat
    23881 ttgcccgttg tgatatctat caacgcgtac tgagcgccag acttagcagg tacagacacc
    23941 atgtcgataa gagggatgta gcgcatagag ccgtcagcaa ggcgctccac tagctgcatt
    24001 gactgtttaa cggccatttc agatgtcatg cagaactccc tacctgcccg gcgggcaatg
    24061 atttttttat tccagatatt cgcccaattt cccacgggca atgtgccagc acgttaccag
    24121 acaaattgct acagagaata gtccagagat agtaagcact cttatttagg cgaaagttat
    24181 gcgtatagcc ctgcaagtta atcgccactt gtcttgtagt ccctcgatga ccgctattgg
    24241 ccgaaaacgg cctctcacaa aagtcggcta ttgcccgcca ccccctctca aacccgctag
    24301 tacccggggc ctaacaccgc gatacctgag aacagatgcc ccagttcgtc ccgtgctgtc
    24361 cgcaccagat tagatgcctt cccataccct aatcccgtcc gtgcttttgc ctgaaggacg
    24421 aggaaagcgt catccctcct gttatccctc gcgttaccgc tatagaaccc acagcccgaa
    24481 ctgcaacgcc ggcacagcta atccgctaca cccttggtaa cccggtactc acccggcgtc
    24541 atgcccgcat tgcgggtgaa gaagcggctg aagtaggccg ggtcggtaaa gcccagctgg
    24601 tagcagattt cgttgaccga gctgccggta aacagcagca ggcgcttggc ctcttgcatc
    24661 aggcggtcga acaccaggcg cttggacggc aggtcggcga tgcgccggca gatgtcgttg
    24721 agccgcgcct cggtcacccc catctgctcg gcatagcgct gcagcgccca gtgcgcctgg
    24781 tagttggcct cgatcagggc attgaaacgc tggaatatct gcagatcttc gcggcgcgct
    24841 ggctgcgact ggatcgagcg cgtcgacagg cgcagcaggc tgataaagat cagccgggtc
    24901 agcgctgcca ggctcagcgc ccgccccggc ctatcgtctg cgaactcgct gcgcagctcg
    24961 tcgaacagca gattgaggcg cgccacttcg gtgtcgtact gcgcatccag ctcaccgacc
    25021 gccacgcaca gcggggcgat ctgcggcccc agggccaggc cctgctgccc ttccagcagc
    25081 ggccagatca gctcctggcg caccgtgagt acatggccat cgctgtcggc gtcggtgaca
    25141 aaggcatggg gaaaggtcgg cggggtgagg aagaacatcg gtccttcctg ctgataacgc
    25201 tgatcatcca ggtacacgcg caccacgccg ctcttgacgt aatgcacctg aaaaaaacgg
    25261 tcgtggcgat gcaccggcat attgcggccg aaaaagtccg ccaacttgcc aagggcctcg
    25321 tagtgcacgt cggcatcggc gtaacgctgg tcgtagacct ggccgatatt gatattgggg
    25381 atgggttgtg ggtcgcgcat gggccttacc ctcgtcgcga gatcgtggtg gcgcggcgca
    25441 gtggccgcgc ccgccaagct cagggtttga tctgcttgag ctcgttgagc agcccaagca
    25501 attgctgcag cttttcctcg ccaaactgtt cctggatccg ctggtagttg ctctccatat
    25561 ccgcactcat cgacacaaag cactcatggc cctgtgcggt cagatcgata tacacccggc
    25621 gctggtcgtg cggcggcttg ctgcggctga ccaggccatc gcgctccagc cggctgagca
    25681 cgccggtcat gctcggtttg aggatgcagg ccagctcggc caggcggtag ctctccattt
    25741 cgccatgctg gcgcaagatg cggatcaccc gccattgctg ctcggtcagg ccatgctggt
    25801 tgagcagcgg gcgaaagaaa ctcatcgcgg cctcgcgggc ctggagcaag gtcagggtca
    25861 gggacggtcg ggtaacaggc atggtgagat tcggcttcag gcggttgtga ataggttaac
    25921 ggtatcactt tcagcctgca caagcgctca cctgcggtca atcggatgga tcgccctggc
    25981 agccagggac ataccgctgt gcaggcagag tcaagcccac ccacagcacc cggcctaagc
    26041 ttggttcctg tccatgccta ccccacagga gcccggttta tgaccgccgc attgatcgaa
    26101 accctcaagt ccgtcgaaga cttcctccag cgccagcacg gctcgtttat cgacggcacc
    26161 tgccagctcg acagcgccag cgaaaccctg gccttgcgca acccggccga cgaccaggtg
    26221 ctggccaatg tgcaactggc cgatgccggc caggtcgacc gcatcgtcga gtccgcccac
    26281 cgcgccttca ggtccggcgt ctggagccgc ctggcaccgg ccgaacgcga acgcaccctg
    26341 ctgcgcttcg ccgacctggt ggaagcccac ggcgaggagc tggcgcagct ggaaaccctc
    26401 aaccagggca agtcgatcca tctcgcgcgc gccatcgagg tcggcgcgtc ggtcaattac
    26461 atgcgctata tggccggctg ggccaccaag atcgagggcc agaccctcga tgtgtccatc
    26521 ccgatgccgg caaacgcgcg cttcaatgcc tacacccggc gcgagccgac gggtgtggtg
    26581 gcgggcatcg tgccgtggaa ctttccgatg atgatcgcct tgtggaaggt catcccggcc
    26641 ctggcctgcg gctgcaccat cgtcatcaaa ccggccgatg aaaccccgct gaccgccctg
    26701 cgcctggccg agctggccat cgaagccggc atccccgccg gcgtgttcaa cgtgctgatc
    26761 ggccgcggtg cccaggccgg tgccgcgctg gtggcacatc cgctggtgaa caaggtgtcc
    26821 ttcaccggct ctaccgccgt gggcaagcag atcggcatcg ccgccctgca caacatgacc
    26881 cgcttcaccc tcgaactggg tggcaagaac ccgatgctgg tgcttgaaga tgccgatatc
    26941 gacaaggccg tggccggtgc catcgctggc ggcctgctca accagggcca ggtctgtgcc
    27001 gcggcctcgc gtctgtatgt gccggccagc cgccatgccc agttcgtcga ggcgctgagc
    27061 gctcaggcca gcgccatgag cctaggcccg ggcatggacc caactgccca ggtcaacccg
    27121 ctggcaacgc gcaagcacca gaaccacgtc cttgccatgc tcgccctcgc ccgccgcgac
    27181 ggcgcacgca ttgccaccgg tggcgacgcc ccggaccgcc ccggttactt cgtcaacccc
    27241 acggtgctca gcggcctgga gcaacgcagc agcacagtgc aggaagaaat cttcggcccg
    27301 gtgctcagcg tgctgcccta cgacgatctg gagcagggcc tggccctggt caacgacagc
    27361 cgctacggcc tggccgccag cgtctggagc aacgacctgg gcaaggtcat ggatctgttg
    27421 ccgcgcatcg aggccggcac cgtgtgggtc aacaaccatg tgccggtcga ccccaacctg
    27481 ccgttcggcg gcttcaaaca gtcgggcatg ggccgcgaat tcggccgcag cgccatcgaa
    27541 ggtttcaccg agctgaagtc ggtgtgcatc acctactgag ggcccgcgag cgcgccccga
    27601 tcaggggccg ctctagccat gccatgcgcc atgaaaagtg caattcaatg gctgtaaaag
    27661 ccattcaacc ctcagcagca gccgtcatta tggtgaccag atagctgccg aatatccgcc
    27721 gctggactgc ctatagaagc agcggcgact cagcaaagta gaagctgtac agatcaataa
    27781 ttaacatgtt aaacatatga ttaacatgtt aaccaaatgc cgcgcttgct tacgcgcagc
    27841 cccgacttta ttgcaatggg agaacaaccg atgagcagtg ctctcgataa ggcagccgcc
    27901 ggcaccctgt tcggcatcgc gctgaactac caggggcttc tgcgcagccg cctggaagaa
    27961 ttccagcggc cgccctacca gaagccgccg gtcaaacccg tgctgtttat caagacctcc
    28021 aacacccgca acggccacga ccagccggtg gtgttccctg ccggcgtcga gcgcctgcaa
    28081 ccgggcccgg cgctgggcgt ggtgatcggc aaggacgcca gccgggtcag cgaggccgag
    28141 gccatgaatc acgtggccgg ctacctgatc gtcaacgaat tcagcctgcc cgaagacagc
    28201 tactaccgcc cggcggtcaa ggccaagtgc cgcgacggct tctgccccat cggcccggaa
    28261 ttggtgcccg ccagccagat caccaacccg aacgccctgg gcatccgcct gttggtcaac
    28321 ggcgaactgc gccagcagaa cagcaccgcc aactgggtgc gcaacatccc ccagctgatc
    28381 gccgaactga gcgagtttat gaccctgcac aaaggtgacg tgctgattac cggcactccg
    28441 gaaggtcgcg tcgatgtgca gcccggcgac attgtcgcag tggaaatcga tggccttggt
    28501 cgtctcgtca ataccgtggt cgctgaagga gcgctctgat gaaacacgcc cgtatccgtt
    28561 tccagggtga catccacgcc gtcaccgtcg aagacaacaa cgccgtgcgc ctggccgacg
    28621 gccgcatgct ggctgaagag gcggtcgaat ggctgccacc ggccagcggc agcatgttcg
    28681 ccctcggcct gaactacgcc gaccacgccg ccgagctggc cttcagcccg cccaccgaac
    28741 cgctggcctt tatcaagtca ccgggtacct acaccggcca caaccagatc acctggcggc
    28801 cggacaacgt cgcctatatg cactacgagt gcgaactggt ggcggtgatc ggcaagccgg
    28861 cgcgcaacgt caagcgcgag gacgcccttg agtacctagc cggctacacg gtgtgcaacg
    28921 actacgccat ccgcgactac ctggaaaact actaccggcc caacctgcgg gtgaagaatc
    28981 gcgactgcac caccccggtc ggcccgtgga tcgtcgacgt cgccgatgtg cccgatccat
    29041 cgaaactgcg cctgcgcacc tggatcaacg gcgagctgaa gcaggaaggc accaccagcg
    29101 acatgatttt cgacatcccc tacctgatcg aatacttctc cagtttcatg accctgcaac
    29161 ccggcgacat gatcgccacc ggcacgccgg aaggcctggc cgacgtggtg cctggcgatg
    29221 aagtggtggt ggaagtggaa ggcgttggcc gcctggtcaa ccgcatcgtc agcgaagccg
    29281 atttcttcac ccaacaagaa ctcgcgcgca acaagcaaga ggcataagca atgatcaagc
    29341 actggatcaa cggccgcgag gtcgagagca aagaaacctt taccaactac aacccggcca
    29401 ctggcgaggc tatcggtgaa gtagccagcg gcggtgccga ggaaatcaac caggcggtcg
    29461 ctgcggccaa ggccgccttc cccaagtggg ccaatacccc ggccaaagaa cgcgccaagc
    29521 tgatgcgcaa gctcggtgag ctgatcgaac agaacgtgcc caaactggcc gaactggaga
    29581 ccctggacac cggcttgccg atccaccaga ccaagaacgt gctgatcccc cgcgcctcgc
    29641 acaacttcga cttcttcgcc gaagtttgca cgcgcatgga cggccacagc tacccggtcg
    29701 acgaccagat gctcaactac accctgtacc agccggtggg tgtgtgcggc ctggtctcgc
    29761 cgtggaacgt gccatttatg accgccacct ggaagaccgc accctgcctg gccctgggca
    29821 acaccgcagt actgaagatg agcgagctgt cgccgctgac cgccaatgaa ctcggccgcc
    29881 tggccctcga agccggcatc ccggaaggtg tgttcaacgt catccagggc tacggtgcca
    29941 ctgccggcga tgccctggtt cgtcacccgg acgtgcgcgc catctccttc accggcggca
    30001 cctccaccgg caagaagatc atgcaaaccg ccgggctgaa gaagtactcc atggaactgg
    30061 gcggcaagtc gccggtgctg atcttcgatg acgccgacct cgaccgcgcc ctggatgccg
    30121 cgctgttcac catcttctcg ctgaacggcg agcgctgcac cgcaggcagc cggatcttta
    30181 tccaggagac ggtgtacgac cagttcgtcg ccgagttcgc cgcccgcgcc aagcgcttga
    30241 ttgtcggcga cccgcaggac ccgaacaccc aggtcggctc gatgatcacc caggcccatt
    30301 acgacaaggt caccggctat atcaggatcg gcatcgagga aggtgccaac ctgctcgccg
    30361 gcggcctgga gcgtccggcc aatctgccgg cgcatctggc caaggggcag tttatccagc
    30421 ccacggtatt cgccgatgtg gacaaccgca tgcgcatcgc ccaggaggaa atcttcggcc
    30481 cagtggtgtg cctgatcaag ttcaaggacg aagccgaagc cctgcgcctg gccaacgaca
    30541 ccgagtacgg cctggcctcc tatatctgga cccaggacat cggcaaggcc caccgcctgg
    30601 cccgcggcat cgaggccggc atggtgttta tcaacagcca gaacgtacgc gacctgcgcc
    30661 agccattcgg cggcgtgaag ggctccggca ccggccgtga agggggtgaa tacagcttcg
    30721 aggtgttcgc cgagatcaag aacgtgtgca tttccatggg cagccaccat atcccgcgct
    30781 ggggcctgtg atgcggcggc gcacccatta gtggtgcgct atctataaat ggaagggggc
    30841 acgtatcccc ttcccctgtg ccggtcgtgc aaacgtcctt acaacaaaag cctcacgcga
    30901 ggccaacagg agaaccgcca tgggcaaagt cgctctagct gccaagatca cccacgtacc
    30961 ctcgatgtat ctttccgagc tgcccggccc gcgccagggc tttcgccaag cagccatcga
    31021 cggccatatc gagatcagca agcgctgccg cgaactgggc gtggacacca tcgtggtgtt
    31081 cgacacccac tggctggtca acgccaacta ccacgtactc tgcggcccgc acttcaaggg
    31141 cctgtacacc agcaacgaat tgccgcactt catcagcaat atggagtacg agttcccggg
    31201 caacccggcg ttgggcaagc tgctcgccga cgaatgcaac cgcttcggcg tcgagaccat
    31261 ggcccacgat gccacgaccc tgggcccgga atacggcacc ctggtgccca tgcgctatat
    31321 gaaccaggac cagcacttca aggtggtgtc ggtctcggcg ctatgcacct cgcactacct
    31381 gaacgacagt gcgcgcctcg gttgggccat gcgcaaggcg gtggaggagc actacgacgg
    31441 caccgtagcc ttcctggcca gtggctcgct gtcgcaccgc ttcgcccaga acggtcaggc
    31501 cccggacttc gccaccaagg tctggagccc cttcctggaa accctcgatc accgcgtggt
    31561 gcagatgtgg caggacggcg aatgggacgc cttctgcggc atgctccccg agtacgcggc
    31621 caagggccac ggcgaaggct ttatgcacga caccgccatg ttgctcggtg ccctgggctg
    31681 gtcgaactac gacgccaagg ccgaggtggt gacgccctac ttcggctcct ccggcaccgg
    31741 ccagatcaac gccatcttcc cggtgatccc gcaggacggc tccgccattc ctgccgccca
    31801 ggccagcaac ccggccggtg tcgtttccac cagccgcctc tgatccctgt gggagcggct
    31861 cagccgcgaa tagcgtctgg ctgaagccct tctgcgattg gcggctgaag cggaacgccg
    31921 cccgaccgct cctactttta cgaggacatc accatgcccc atctggttct gctctacacc
    31981 cccgacatcg agcgtgacct ggacgtcagc ggcctatgcc gcgccctggc cgactgcatg
    32041 ctcgaacagc gtgacgaggc cggcaaagcc gtattcccta ccggcggcac ccgtgtactg
    32101 gcctacccgg cagcccatgc cgcggtggcc gatggcaagg gcgactacgg ttttctctac
    32161 gccaacctgc gtatgggggc cgggcgtagc caggtggtgc ataaacaggt gggcgacaac
    32221 ctgatgcatg tgttgcgcgc gcgcctcgac ggcctgctcg cccagcgccc gctgggcgtc
    32281 accctgcaga tcgacgaaag cccaggccag gtcttcgatg ccaagcacag cagcctgcat
    32341 cccctattca acaaagctcc ctaaggtaat cctgccatgc tcgacgcctc catcattcaa
    32401 caggccgccg cccgcctcga cgccgccgag cgctcacgcg aacaggtgcg ccagttttcc
    32461 ctggactacc caaacatcac catcgaagac gcctacgcca ttcagcgtgc ctgggtcgat
    32521 ctgaaactca aggccgggcg caagctggtc ggccacaaga tcggcctgac ctcgcgcgcc
    32581 atgcaggtgt cgtcgaatat caccgagccg gactacggtg ccctgctcga cgacatgctg
    32641 ttcgaggaag gcagcgacat tcccttcgag cgctttatcg tgccgcgggt agaggtggag
    32701 ctggcgttta tcctcggcaa gccgctgcgc gggccgaact gcagcatctt cgacgtgctg
    32761 gaggccactg aatgggtggt gcccgcgctg gaaatcatcg atgcgcgcat ccagcaggtc
    32821 gacccggaca gcaaagttac ccgcaaggtg ttcgacacca tttccgacaa cgctgccaac
    32881 ggcggcgtgg tcatgggcgc gcggccggtg cggcctaacg atatcgacct gcgcagggtg
    32941 ccggcgattc tctaccgcaa cggcgtgatc gaagaatccg gtgtctctgc cgcggtgctc
    33001 aaccacccgg ccaagggcgt ggcctggctg gccaacaaac tggcccccta cgacgtcacc
    33061 ctgcaagccg ggcagatcat cctcagcggt tccttcactc gcccggtggc ggccagcccc
    33121 ggcgatacct tccacgttga ctacgaccaa ctcggcagca tcgcctgccg ctttgtctag
    33181 ccccacaaac tgtaggagcg agcttgctc
//
LOCUS       DASRUM010000020         3364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2186341, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000020 DASRUM010000000
VERSION     DASRUM010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2186341"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1119
                     /gene="epd"
                     /locus_tag="VFF22_01410"
     CDS             43..1119
                     /gene="epd"
                     /locus_tag="VFF22_01410"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HZX15511.1"
                     /translation="MPKSKRPYRIALNGYGRIGRCVLRALHERGAGAQLEIVALNDLA
                     DQASIEYLTRFDSTHGRFPGEVRVDGDCLHINGDCVKVLRQATPEAIDWAALDIDLLL
                     ECSGQYTSRADAQRFIDAGAPRLLLSQPMASEADIDATVVFGVNQDCLSGREQLVSNA
                     SCTTNCGVPLLKLLNETVGIEYVSITTIHSAMNDQPVIDAYHHEDLRRTRSAFQSVIP
                     VSTGLARGIERLLPELSGRIQAKAIRVPTVNVSCLDITLQTARDTSAAEINAVLRQAA
                     ESGPLKGLLAYTELPHASCDFNHDPHSAIVDGSQTRVSGPRLVNLLAWFDNEWGFANR
                     MLDVAEHFLLQACPTPFNQPPVKD"
     gene            1124..2287
                     /locus_tag="VFF22_01415"
     CDS             1124..2287
                     /locus_tag="VFF22_01415"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005896277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZX15512.1"
                     /translation="MTVLKMTDLDLAGKRVLIREDLNVPVKDGVVKSDARILASLPTI
                     KLALEKGAAVMVCSHLGRPTEGEFSAENSLKPVADYLSKALGRDVPLVADYLNGVELK
                     AGEIVLFENVRFNKGEKKNADDLAQQYAALCDVFVMDAFGTAHRAEGSTHGVAKFAKV
                     ACAGPLLAAELDALGKALGNPAKPMAAIVAGSKVSTKLDVLNSLSQICDQLIVGGGIA
                     NTFLAAAGFPVGKSLYEADLVDTAKAIAAKVSVPLPVDVVVAKAFAEDAEATVKLVAD
                     VAEDDMILDIGPQTAAQFAELLKSSKTILWNGPVGVFEFDQFGNGTKVLAQAIAESPA
                     FSIAGGGDTLAAIDKYGVGAQISYISTGGGAFLEFVEGKVLPAVEVLEQRAKA"
     gene            2458..>3364
                     /gene="fba"
                     /locus_tag="VFF22_01420"
     CDS             2458..>3364
                     /gene="fba"
                     /locus_tag="VFF22_01420"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249246.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HZX15513.1"
                     /translation="MALISMRQMLDHAAEFGYGVPAFNVNNLEQMRAIMEAADKTDSP
                     VIVQASAGARKYAGAPFLRHLILAAIEEFPHIPVCMHQDHGTSPDVCQRSIQLGFSSV
                     MMDGSLKEDGKTPADYDYNVAVTQQTVAFAHACGVSVEGELGCLGSLETGMAGEEDGV
                     GAEGVLDHSQMLTDPEEAADFVKRTQVDALAIAIGTSHGAYKFTKPPTGDILAIDRIK
                     EIHKRIPNTHLVMHGSSSVPQEWLAIINQYGGDIKETYGVPVEEIVEGIKYGVRKVNI
                     DTDLRLASTGAMRRLMATNPSEFDPR"
BASE COUNT          662 a         1074 c         1026 g          602 t
ORIGIN      
        1 gagcaagctc gctcctacag gttgactgca ttcgtgccaa acatgccaaa aagcaagcgc
       61 ccctatcgaa tcgccctcaa cggctacggc cgtatcggcc gctgcgtgct gcgtgcgctg
      121 catgagcgcg gtgcgggcgc gcagctggaa atcgtcgcgc tgaatgatct ggccgaccag
      181 gccagcatcg aatacttgac ccgctttgac tccacccacg ggcgttttcc cggcgaggtg
      241 agggtcgacg gcgattgcct gcatatcaat ggcgactgcg tgaaagtgtt gcgtcaggca
      301 acgcctgagg ccatcgactg ggccgcgctg gatatcgatc tgctgctgga atgctcgggc
      361 cagtacacca gtcgcgccga tgcccagcgc tttatcgatg cgggcgcgcc acggttattg
      421 ctgtcgcaac cgatggccag cgaggcggat atcgatgcca cggtggtgtt tggcgtcaat
      481 caggattgcc tgagcggccg cgagcagttg gtgtcgaacg cctcctgcac caccaactgt
      541 ggcgtgccgc tgctcaagtt gctgaatgag acggtgggca tcgagtacgt gtcgatcacc
      601 accatccact cggcgatgaa cgatcagccg gtgattgatg cctatcacca tgaagatctg
      661 cgccgtacgc gttcggcctt ccagtcagtg attccagtgt ccaccggtct ggcccgcggt
      721 attgagcgcc tgttgccgga actctctggg cgtatccagg ccaaagccat acgtgtgccg
      781 acagtgaatg tttcgtgtct ggatatcacc ctgcagaccg cccgcgatac gtcggcggcc
      841 gagatcaacg cggtgctgcg ccaggcggcc gagagcggtc cgttaaaagg gctgttggcc
      901 tacaccgaac tgccccacgc cagctgcgac ttcaaccacg acccgcattc ggcgattgtc
      961 gatggcagcc agacccgtgt gtccggcccg cgcctggtga acttgttggc ctggttcgac
     1021 aacgagtggg gctttgccaa ccgcatgctg gatgtcgccg aacattttct cctacaagcc
     1081 tgtccaaccc cgttcaacca gccccccgtg aaggactgat ttaatgactg ttctgaagat
     1141 gaccgacctc gacctcgccg gtaagcgcgt gctgatccgc gaagacctca acgtgccggt
     1201 aaaagacggc gtggtgaaga gcgacgcgcg catcctcgct tccctgccga ccatcaagct
     1261 ggcgctggag aagggcgcag ccgtgatggt ctgctcgcat ctgggtcgcc cgaccgaagg
     1321 tgaattcagc gcagagaaca gcctcaagcc cgtcgccgac tacctgagca aggccctggg
     1381 ccgcgacgtg ccgctggtgg cggactacct gaacggtgtc gagctcaagg ctggcgagat
     1441 cgtgctgttt gagaatgtgc gctttaacaa gggcgagaaa aagaacgctg acgatctggc
     1501 ccagcaatac gccgccctgt gcgacgtgtt cgtcatggac gccttcggca ccgcccaccg
     1561 cgccgagggt tcgacccatg gcgtggccaa gttcgccaaa gtcgcttgcg ccggcccgct
     1621 gctggcggcc gagctggatg ccttgggcaa ggcgctgggt aacccggcca agccgatggc
     1681 ggcgattgtt gccggctcca aggtgtcgac caagctcgac gtgctcaaca gcctgagcca
     1741 gatctgcgat cagctgatcg tcggcggtgg catcgccaac accttcctcg ctgccgccgg
     1801 tttcccggtt ggcaagtcgc tgtatgaagc cgatctggtc gacaccgcca aggccatcgc
     1861 ggccaaggtc agcgtgccgt tgccggtcga cgtggtcgtc gccaaagcct tcgccgaaga
     1921 cgccgaagcc accgtcaaac tggttgccga tgtggctgag gacgacatga ttctggatat
     1981 cggcccgcag actgcagcgc agttcgccga gttgctgaaa tcctcgaaga ccatcctgtg
     2041 gaacggcccg gttggcgtgt tcgagttcga ccagttcggc aacggcacca aggtgctggc
     2101 ccaggccatc gcagaaagcc cggcgttctc catcgcgggc ggtggcgaca ccctggcagc
     2161 catcgataaa tacggcgttg gcgcgcagat ttcctatatc tccaccggtg gtggcgcatt
     2221 cctcgagttc gtcgagggca aagtgctgcc ggctgtggaa gtgctggaac agcgcgccaa
     2281 ggcctgagag accggccgcg gccgggttat gccaatattc atgtaggagc gggccatgcc
     2341 cgcgaaacgg gcaacgcaaa agcttcgcgg gcatggctcg ctcctacaaa ttttcaaccg
     2401 cgccgccact acggcgggct gctacgatta accagcccca ccgggagaaa gaacaccatg
     2461 gcacttatca gcatgcgtca gatgctcgac cacgccgccg aattcggcta cggcgtgccg
     2521 gccttcaacg tcaataacct cgagcagatg cgcgcgatca tggaagcggc cgacaagacc
     2581 gattccccgg tgatcgtcca ggcttccgcc ggtgcacgta aatacgctgg tgcgccgttt
     2641 ctgcgccacc tgatcctggc cgccatcgaa gagttcccgc atatcccggt gtgcatgcac
     2701 caggaccacg gcaccagccc tgacgtctgc cagcgctcga tccagctggg cttcagctcg
     2761 gtaatgatgg acggctcgct gaaagaagac ggcaagaccc cggctgacta cgactacaac
     2821 gtcgccgtga cccagcagac cgtggccttt gcccacgcct gcggcgtgtc ggtggaaggt
     2881 gagctgggtt gcctgggcag cctggaaacc ggcatggccg gcgaagaaga cggcgtcggc
     2941 gccgaaggcg tgctggatca cagccagatg ctgaccgacc cggaagaagc cgccgacttc
     3001 gtcaagcgca cccaggtcga tgccctggct atcgccatcg gcaccagcca cggcgcctac
     3061 aagttcacca agccgcccac cggtgacatc ctcgccatcg accgcatcaa ggaaatccac
     3121 aagcgcatcc ccaataccca cctggtgatg cacggttctt cttcggtgcc gcaagagtgg
     3181 ctggcgatca tcaaccagta cggcggcgac attaaagaaa cctacggcgt accggttgaa
     3241 gaaatcgtcg aaggcatcaa gtacggcgtg cgcaaggtca acatcgacac cgacctgcgt
     3301 ctggcgtcca ccggtgccat gcgccgcctg atggccacca acccgagcga gttcgacccg
     3361 cgca
//
LOCUS       DASRUM010000021        15436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_347868, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000021 DASRUM010000000
VERSION     DASRUM010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 15436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15436
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347868"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..986
                     /locus_tag="VFF22_01425"
     CDS             3..986
                     /locus_tag="VFF22_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HZX15514.1"
                     /translation="MQVKHLLLIAILALTVACSSKPVVDENLSEAELYQQAQADLDNS
                     SYTSAVAKLKALESRYPFGRYAEQAQLELIYAYYKNIEPEAAKSAADRFIRLHPQHAN
                     VDYAYYLKGLASFDQDRGLIARFVPLDMTKRDPGAARDSYNEFAQLTSRYPTSRYAPD
                     AKQRMIYLRNLLAAYEIHVAHYYLTRNAYVAAANRGRYVVENFQETPAVGDGLAVMTE
                     AYQRLTLDDLAATSLETLKLNYPDHPSLVDGQFVPREEEADSRSWMAKATLGLIETDT
                     PLPPGETRASQDVIRQYEDAEEQIPDELKTGNSEEQSSRSWFSYLTFGLFD"
     gene            1198..1443
                     /locus_tag="VFF22_01430"
     CDS             1198..1443
                     /locus_tag="VFF22_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HZX15515.1"
                     /translation="MGLFRLLFWIAIIAAAFWLWRRFTRPAAPRSKDHNASAAPTPMV
                     RCEHCGTHVPRDHALAKGQQWFCSQAHLEQGPQSGER"
     gene            1433..3022
                     /locus_tag="VFF22_01435"
     CDS             1433..3022
                     /locus_tag="VFF22_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX15516.1"
                     /translation="MNADFSLSGEQSRRILRLYHLYRLVIGITLALLVSSNLDSELLD
                     MMHPTLFRNGSWLYLALNALIIATVRHPKGLTQVFSLALVDVILLSGLFYAAGGTPSG
                     IGNLLIVAVAIANILLRGRIGLLIAAVAATGMIYLTFYLSLSRPAAAAQYVQAGALGA
                     LCFAAALFVQGLTRRLQVSENLAEQRAADVANLEALNALILQRMRTGILVLDELHRVL
                     LANQGANHLLGHADLTGKILDPHCPELIKRMQQWSHNPSLRPASLQAAPDGRVLQPSF
                     IALQRGEHKHTLIFLDDISQIAQQAQQLKLASLGRLTAGIAHEIRNPLGAISHAAQLL
                     QESEELEGPDLRLAQIIQDHSRRMNLVIENVLQLSRRRQAEPQLLDLKYWLHRFASEF
                     RSSAAPGQTLHLETSSGSIQTRMDPHQLTQVLSNLVENGLRYSAQKNKQGQVWLKLFR
                     DPESELPVLEILDDGPGIAEEQLHHLFEPFYTTENKGTGLGLYISRELCESNQARLDY
                     KLREGGGSCLRITFAHPRKLS"
     gene            3025..4362
                     /locus_tag="VFF22_01440"
     CDS             3025..4362
                     /locus_tag="VFF22_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZX15517.1"
                     /translation="MTRQRALIVDDEPDIRELLEITLGRMKLDTRSARNVKEAREWLA
                     KEPFDLCLTDMRLPDGTGLELVQHILLRHPQVPVAMITAYGSLDTAINALKAGAFDFL
                     TKPVDLARLRELVAAALRLRSTDSAELPVDSRLLGDSPPMRAMRKQILKLARSQAPVY
                     ISGESGSGKELVARLIHEQGSRGEQPFIPVNCGAIPTELMESEFFGHKKGSFTGAIED
                     KQGLFQAANGGTLFLDEVADLPLPMQVKLLRAIQEKAVRAVGGQQELIVDVRILCATH
                     KDLAGEVAAGRFRQDLYYRLNVIELRVPALRERREDIALLADIMLKRLAEGTGLSPAK
                     LDDDALEKLKSYRFPGNVRELENMLERAYTLCEDDRITTCDLRLADSVPISENGEANL
                     AQIDNLEDYLEAIERKLIMQALEETRWNRTAAAQRLGLSFRSMRYRLKKLGID"
     gene            complement(4417..5520)
                     /gene="thiO"
                     /locus_tag="VFF22_01445"
     CDS             complement(4417..5520)
                     /gene="thiO"
                     /locus_tag="VFF22_01445"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HZX15518.1"
                     /translation="MLDVIVVGGGVLGLLTASNLAAVGWRVGVMESGCLGHESSWAGG
                     GIVSPLYPWRYSPAVTALAHWSQDFYPRLGEQLLAQTQIDPEVYVTGLYWLDLEDEAE
                     ALAWAQREGRPLRRVDVAAVERAVPALAEGFGRAVQMADVANVRNPRLVKALRASLQQ
                     MPNVTLHEQCAVTGFIHEDGRITGVHTARGDMHADQVVLAAGAWSGELMKTLGIELPV
                     VPVKGQMILYKCAEDFLPAIVLAKGRYAIPRRDGHILIGSTLEHAGFDKTPTDEALAS
                     LKASAVELLPALADAEVVGHWAGLRPGSPEGIPFIGPVPGHEGLWLNCGHYRNGLVLA
                     PASCQLLADLMLGREPIVDPAPYAPAGRLASAG"
     gene            5607..6128
                     /locus_tag="VFF22_01450"
     CDS             5607..6128
                     /locus_tag="VFF22_01450"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HZX15519.1"
                     /translation="MRTDSIAGYSLLDVLITLALIGTLIGIGTPALSSWSEQQRLRSL
                     QTNLFHMTNKARYLAMTSQHRVTLCPLSEQGKCQQSWSGSISSFIDSNGNRQLDAGEE
                     IFSTLYVPNSITLNWSGMLPKNSIHFSNQGVTFVSNGTMSLCPTRPDSHSGTMVINRQ
                     GRVKTSKDGAHCP"
     gene            6242..6760
                     /locus_tag="VFF22_01455"
     CDS             6242..6760
                     /locus_tag="VFF22_01455"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HZX15520.1"
                     /translation="MMKQQSGFTLVEMMVTIAILAILMGIAAPAVTDFAIKQRVSSQA
                     NEMMLSLAFARSEAVKLNQDVRVIPRTSTANGWSDGWCVGPANIGSDCNHADVIRVFT
                     GAGGVSITSSGISNPPALVFLRDGTASNSVDALLKVSSPNLEPAGDTARCIRLTRPGR
                     AEVEKVARDAAC"
     gene            6777..7358
                     /gene="pilV"
                     /locus_tag="VFF22_01460"
     CDS             6777..7358
                     /gene="pilV"
                     /locus_tag="VFF22_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HZX15521.1"
                     /translation="MSPLLYKQHGATLIEVLVALLIFTVGLLGLAAMQLSSLQSTADS
                     GQRSQSVWLMQDFIERMRTNPDGTAAQYAAPQNCAALPARMCADHYNPSTAAKVNASE
                     CNATQMAAFDAWEAQCSYAAIATFNTIDARFNSRDFITPPVAGTLVDAIADGNNLDLN
                     LNWLNRSNLSPKSQAQAAETETDGTTTVLRVER"
     gene            7355..8239
                     /locus_tag="VFF22_01465"
     CDS             7355..8239
                     /locus_tag="VFF22_01465"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HZX15522.1"
                     /translation="MNPREHRQRSRQRGFSLVELLVAFVISMLLLTGVIQVFFASKQT
                     YASNEAASRLQENGRFALEFIAQSARHAGYVQPDNNADKPSPIAKPGDISCDSNNNED
                     DACSNDGDNAAADIWERSDRTSFIFQPRLGADDKRRDCNGNEIANNNALILNQFFIAP
                     PSAITPPYDPTLHPPLTLNCRSLIFNSNSKGAFIGVQSPLIEGIDALQIQYGLSEKSS
                     DLNNVSQYISAERVTALNKWSNVTAIRIAVLANSVERTNPPTPARAYVLLDAAPIVID
                     DGRARQVFTTTIKLRNNF"
     gene            8250..8819
                     /locus_tag="VFF22_01470"
     CDS             8250..8819
                     /locus_tag="VFF22_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HZX15523.1"
                     /translation="MMNHTPSQQTGATLIVALVFLVVLTIAGITAMQFSTLEERMASN
                     SQFRNETFQQAQSSIQANLIALSANATGRDRLLSALNAASNSSADSDCDEDYPFNLCT
                     QKLEQLGLQPTAKQAIDAIEDQTANLGFSVIRNTAPAKACEDDVYGASSITNFECINY
                     ELQIREELPNGAYSDQSQGLTFMNIKAKS"
     gene            8831..9157
                     /locus_tag="VFF22_01475"
     CDS             8831..9157
                     /locus_tag="VFF22_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15524.1"
                     /translation="MVKSITSLILILSFISTISISHAETANNSRTTIEGANVTIRMTN
                     NQPYAEFQPCDDCPYRLLSFTPTAQIYLNGEKTTASALQDGQTMEGTVFIQQRPIESI
                     IEIVAN"
     gene            9171..13700
                     /locus_tag="VFF22_01480"
     CDS             9171..13700
                     /locus_tag="VFF22_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HZX15525.1"
                     /translation="MNIRSLSIVLGSFCYISMTLQTALADDTEIYVSRKLPEDQQVRP
                     NLMFVIDNSGSMLSNVPGTTCAVADRSIRNRCEEVLISQTCTRYDWRGRCDRWTNNYE
                     YRKTRLQVVKDVTNELVEELRLSDDVNIGLMHFDAAPNTSNEEGAMVAIAAGRASTTA
                     NSFIAELNRLYGASNTPLSESFYEAALYMKGATPKFGNGTNAAYEDGNSWTNPSKPSV
                     AASKSGSTYKSPIQYSCQKSNIILLTDGEPTYDNSANNNIRTLINTSGAANTQHLAST
                     CRDPGTSSTSRSGECMPHLAEHLANQDASSSQTGKQTINTYTIGFATNQTLLQETATA
                     GNGRYFTTDNTSGLVDALKSILVEILAENSTFATPSVAVSAYNNLGYRNDLYYALFRP
                     AEGARWVGNVKRYKLGEDSTSGDAIIVDKNGQPAVDETTGFFRENASSYWSFQDGRDV
                     AKGGVAGMLGNPADRKIYTWTAADRSPTAGAGVTGSVLINTSAHALTTANAAITTALM
                     GVSTAAAKDNAVKWGRGLDPVTNQARQQLADVLHNEPRLVAYKTDEDLVRVENIKNGT
                     ASPSTSPEELYMFFGTNEGFIHAVSPQTGEEKFSFIPKELLPNLNAYYTDPKGSDAKR
                     YGMDGQINLWVEYGAINTTTKSRSITKNYLYAGMRRGGRNYYALDVGTITSPALKWVI
                     KGGTTPGFEKLGQTWSTPKLADINVNSTKTKVLIFTGGYDPQQDDDGTNTPLANDNYG
                     NSLYIVNAETGQLIWRAGHTSETGANLQLSNMTHSMPADPVIIDINSDGLADIIYATD
                     TRAQVFRFDINNANAGASDLGVGGRIASLGGANALNNRRLFNTPDVALVRERGSKTYF
                     TISLGSGYRAHPLNEDTIDRFYVLRDENVFNKPTSYAYAATASTPDKPITESDLVDVS
                     SVDLTDAQAAEVLEEIAELEAKINALNQSVADTRDVFTAFKNSSGFTDKYNAMLQANS
                     SANDRQVAIDSLMASDPYLLAHAPESLEQSKLQQGLLASQQTLQAFQQARTAADSDLA
                     AKEAAYNSASADKATAVAAREAAQTAYDADNSPENQTNLDNATAAETAAINAYASASA
                     AAQAAFDKKNTLHTQAESLAGLYSTLTNLQSELSSRHQQILDKEQAIIAAKATAADET
                     AIASLETELAGLNSSYASDPLTTERDALNAGATSTVLEDALAQLNTAITGGDTSTVQT
                     ALDNLETLAGPPTGPAQSIEDLLSRDEAAKNTELANNAENQASNVALLDTLDSQRLAF
                     AGQASTAQAEANAIANAAYDEGSALLTAAQLAAAKALYGSDLTIFEAYQYLIDQAQLA
                     TLDEDKGLPALRESINSLYAQLTPGNNYVPNKTLLANSKGWFLRLPKGEKVLSSPLIF
                     RGAVFFNTFSPRGETVTTCGPDVGRGRAYALSLKDASAILSRTGATPGSETPIRSYAL
                     TRSGIPPAPSVVFSDNGPPKVIIGTEVLNDRDPSDNSAAECTAGEKCPIKCTGDVGYC
                     PKNPQTIEKTYWREN"
     gene            13700..14155
                     /locus_tag="VFF22_01485"
     CDS             13700..14155
                     /locus_tag="VFF22_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HZX15526.1"
                     /translation="MRQQRGFTLIELMIVIAIIGILAAIAYPSYQEYQLKSGRSDGHA
                     KLMQVMQAQERFYSQNQSYTDNLGATAAGNPGLAFPGTAEDAPVISDEQRYSIAAEVC
                     AVGTPLTRCVRLVATAVGPQVADAQCGNLSLDSRGVKGESGTGTVQACW"
     gene            complement(14230..15174)
                     /gene="ispH"
                     /locus_tag="VFF22_01490"
                     /gene_synonym="lytB"
     CDS             complement(14230..15174)
                     /gene="ispH"
                     /locus_tag="VFF22_01490"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HZX15527.1"
                     /translation="MHIKLANPRGFCAGVDRAIEIVNRALEVFGPPIYVRHEVVHNKF
                     VVEDLRSRGAIFVEELDQIPDDVIVIFSAHGVSQAVRAEAERRGLKIFDATCPLVTKV
                     HMEVVRYSREGRECILIGHEGHPEVEGTMGQYDASNGGAIYLVEDETDVAELQVRNPE
                     ALAFVTQTTLSMDDTSKVIDALRSKFPAIGGPRKDDICYATQNRQDAVKQLAAECDVL
                     LVVGSPNSSNSNRLRELAERMGTPAYLIDGAEDLKQEWFAGVAGIGITAGASAPEVLV
                     QGVIQQLSEWGATGAQELLGRPENVTFSMPKELRMKPV"
     gene            complement(15321..>15436)
                     /locus_tag="VFF22_01495"
     CDS             complement(15321..>15436)
                     /locus_tag="VFF22_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003381292.1"
                     /note="rotamase; accelerates isomerization of the peptidyl
                     prolyl bond, involved in the folding of proteases; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZX15528.1"
                     /translation="PGVVKAFDYNQVTIDFNHPLAGKALSFEVEIIEVKAL"
BASE COUNT         3917 a         4784 c         3876 g         2859 t
ORIGIN      
        1 ccatgcaagt gaaacacctg ctgctgatcg caatcctcgc cctcaccgtc gcatgctcat
       61 cgaagccggt ggtcgacgaa aacctgagtg aggccgaact ctaccagcag gcacaggccg
      121 acctggataa tagtagctac accagcgccg tcgccaagct caaggcgctg gagtcgcgct
      181 atccgttcgg ccgctatgcc gagcaggccc agctggagct gatctacgcc tactacaaga
      241 acattgagcc ggaagctgcc aagtccgcag cagaccgctt tatccgtctg cacccgcagc
      301 acgccaacgt tgactacgcc tactacctca aaggcctcgc ctcgttcgac caggaccgcg
      361 gcctgatcgc ccgcttcgtc ccgctggaca tgaccaaacg cgacccgggt gccgcccgcg
      421 actcgtacaa cgagttcgcc cagctcacca gccgttaccc caccagccgc tacgcgccag
      481 acgccaagca gcgcatgatc tacctgcgca acctgctggc ggcctacgag atccacgtgg
      541 cgcattacta cctgactcgc aacgcctacg tggctgcagc caaccgtggt cgttacgtgg
      601 tggaaaactt ccaggaaacc ccagccgtcg gcgatggcct ggcggtaatg accgaagcct
      661 accagcgcct aaccctggat gacttggccg ccaccagcct ggaaaccctc aaactcaact
      721 acccggacca cccaagcctg gtcgacggcc agttcgtccc gcgcgaagaa gaagccgaca
      781 gccgctcgtg gatggccaag gcaacactgg gcctgatcga aactgacacc ccgctgccac
      841 cgggcgaaac ccgcgccagc caggacgtga ttcgtcagta cgaagacgcc gaagagcaga
      901 ttccggacga actcaaaacc ggcaacagcg aagaacagtc gagccgctcc tggttcagct
      961 acctgacctt cggactgttt gactaagccg gcaaagagca gtgaaagagg gaggcccgag
     1021 gcctcccttt ttattggccg gacggcaaaa ttagaactgc acgcaagtcc accgctcaac
     1081 accgcacagg gcttattgtt caaagtgacg ggaacagcac tcagcgcaca gcttcaacgc
     1141 tgtgcgcaca caggtgggtt agctaaactg cccgcttacc gatagaaaga gagcgccatg
     1201 ggcctgttcc gcttactgtt ctggattgcc atcattgcag ccgcattttg gttatggcga
     1261 cgctttactc ggccagcagc ccctcgcagc aaagaccaca acgccagcgc agcgcctacc
     1321 cccatggtgc gctgcgaaca ttgcggcact catgtgccgc gcgaccatgc cctggcaaaa
     1381 ggccaacagt ggttttgcag ccaggcacat ctagaacaag gcccacaatc cggtgaacgc
     1441 tgatttctcc ctgagcggag agcaaagccg gcgaattcta cggctctatc acctgtaccg
     1501 cctggttatc ggcattaccc tagccctgct ggtctccagc aacttggaca gcgaactgct
     1561 ggacatgatg cacccgacgc tgttccgtaa cggcagttgg ctgtatctgg cactcaacgc
     1621 gctgatcatc gccaccgtgc gtcaccccaa gggcttaacg caggttttca gcctggcctt
     1681 ggtcgatgtg attctgctat ccggcctgtt ttatgccgca ggcggcacac ccagcggcat
     1741 cggcaacctg ctgattgttg ccgtggcgat cgccaatatc ctcttgcgcg ggcgtatcgg
     1801 cctactgatt gccgcagtcg ccgcaacagg catgatctac ctgaccttct acctcagcct
     1861 cagccgacca gctgccgccg cgcagtacgt gcaggccggc gcacttggcg cgctatgctt
     1921 cgccgccgcg ctatttgtgc agggcctgac gcgccgcctg caagtcagcg aaaacctggc
     1981 cgagcaacgc gcagcggatg tcgccaacct ggaagcactc aacgcgctga ttctgcaacg
     2041 catgcgcacc gggattctgg tgctggatga gttacatcgc gtactactgg ccaaccaggg
     2101 cgccaaccac ctgctggggc atgccgatct gacaggcaaa attctcgacc cgcattgccc
     2161 ggagctgatc aagcgtatgc agcaatggtc gcacaacccc agcctgcgcc ctgccagcct
     2221 gcaagcagca cccgatggcc gcgtactgca gccaagcttt attgccctgc agcgcggcga
     2281 gcataaacat acgctgatct tcctcgatga catctcgcaa atcgcccagc aagcgcaaca
     2341 actcaagctc gcctccctgg gccgcctgac cgccggcatc gcccacgaaa ttcgcaaccc
     2401 gttaggcgca atcagccacg ctgcccagtt gctacaagag tcggaggaac tggaaggtcc
     2461 agacctgcgt ctggcgcaga tcattcagga tcactcgcga cgaatgaatc tggtgatcga
     2521 aaacgtcctg caactgtcgc gacggcgcca ggcagaacca caactgctcg acctgaagta
     2581 ctggctgcac cgtttcgcca gcgagttccg cagcagcgcc gccccaggcc agaccctgca
     2641 cctggagacc agcagcggca gcatccaaac ccgcatggac ccacaccagc tcactcaagt
     2701 gctgagcaac ctggttgaaa acggcctgcg ttacagcgcg caaaagaaca agcaggggca
     2761 agtctggcta aagctgttcc gtgacccgga gagcgagttg ccggtgctcg agatactgga
     2821 tgacggtcct ggcattgccg aagaacaact gcaccacctg ttcgagccct tctacaccac
     2881 ggaaaacaag ggcaccggcc tgggcctgta tatttcccgc gaactgtgcg agagcaatca
     2941 ggcccgcctc gactataaac tgcgtgaagg tggcggcagc tgcctgcgca tcaccttcgc
     3001 ccacccgcgc aaactgagct gaccatgacc cgccagagag ccttgatcgt cgatgacgaa
     3061 cccgatattc gcgaactcct ggaaatcacc ctggggcgca tgaagctcga cacccgcagc
     3121 gcgcgcaacg tcaaggaagc ccgcgaatgg ctggcgaagg aacccttcga cctgtgcctg
     3181 accgacatgc gcctgcctga cggcaccgga ctggagctgg tacagcatat tctgctgcgc
     3241 cacccacaag tgccggtggc gatgatcacc gcctacggca gcctggatac cgcgatcaac
     3301 gcgttgaaag ctggtgcatt cgactttctg accaaaccgg tggacctcgc tcgcctgcgt
     3361 gaactggttg ccgccgcctt gcgcctgcgc agcactgata gcgcagagct gccggtggac
     3421 agccgcctgc tcggcgactc accgccaatg agagccatgc gcaagcagat actcaagctg
     3481 gcccgcagtc aggccccggt atatatcagc ggtgagtccg gcagcggtaa ggagctggtt
     3541 gcgcgcctga tccatgagca aggctcacgc ggcgaacagc cctttatccc ggtcaactgc
     3601 ggcgccattc ccaccgagct gatggaaagc gagttctttg gccacaaaaa aggcagcttt
     3661 accggcgcta tcgaagataa acagggcctt ttccaggccg ccaatggcgg cacgctgttc
     3721 ctagacgagg tcgccgatct gccactacca atgcaggtca aactgctgcg cgcgattcag
     3781 gaaaaagccg tacgcgccgt gggtggccag caagagctga tcgtcgatgt gcgcatcctc
     3841 tgcgccaccc acaaggacct ggccggagaa gtcgcggccg ggcgcttccg ccaggatctg
     3901 tattaccgcc tcaacgtaat agaactgcgc gtgcccgccc tgcgcgagcg tcgcgaagac
     3961 atcgctctgc tcgccgacat catgctcaag cgcctggccg aaggcaccgg cctcagcccc
     4021 gcaaaactgg atgacgacgc gctggagaag ctcaagagct accgcttccc tggcaacgtc
     4081 cgcgagctgg agaatatgct cgaacgtgcc tacaccctgt gcgaagacga ccgcatcacc
     4141 acctgtgacc tgcgcctggc cgacagtgtg ccgatcagtg agaacggcga agccaacctc
     4201 gcccagatcg acaacctgga agattaccta gaagccatcg agcgcaagtt gatcatgcaa
     4261 gccctggaag aaacccgctg gaaccgcacc gccgcggcac aacgcctggg cctgagtttt
     4321 cgctcgatgc gttaccggct aaagaagctt ggcatcgact aaagcgagca tcaattgtgc
     4381 gaggggcttt agccgcgcgc cctcccagta gaacgctcaa ccagccgaag ccagccgccc
     4441 agcaggcgca taaggtgcag gatcgacaat tggctcacgc cccagcatca gatccgccag
     4501 caactggcac gacgccggcg ccagcaccag tccattgcgg taatgcccgc agttcagcca
     4561 caaaccttca tgccccggca ccggcccaat aaacggaata ccctcaggcg aaccaggccg
     4621 caaccccgcc caatgcccga ccacctcggc atccgccaac gccggcaata actcgacagc
     4681 tgaagctttt aagctggcca acgcctcgtc agtcggcgtc ttgtcaaaac ccgcatgctc
     4741 aagtgtgctg ccaatcagaa tatgaccgtc acgccgcgga atcgcataac gccccttggc
     4801 cagcacaatg gcaggcagaa agtcttcggc gcacttgtac agaatcatct gccccttaac
     4861 cggcaccacc ggcaactcaa tgccaagggt tttcatcaac tcaccactcc aggccccggc
     4921 tgcgagcact acctgatcgg catgcatatc accacgggcc gtatgcacac ccgtgatgcg
     4981 tccatcttcg tgaataaacc ccgtgaccgc acactgctca tgcagcgtga cgtttggcat
     5041 ttgctgcaac gacgctcgca gcgccttgac caatcgcggg ttgcgcacat tggctacatc
     5101 cgccatctgc accgcccgcc caaagccctc agccaacgcc ggcaccgcac gctctaccgc
     5161 agcaacatcc acacgccgca gcggccgccc ctcacgctgc gcccaagcca gcgcctcggc
     5221 ctcgtcctca agatcaagcc aatacaaccc ggtgacatac acctccggat caatctgcgt
     5281 ctgcgccagc aactgctcac ccaagcgcgg ataaaaatcc tgcgaccaat gcgctagcgc
     5341 tgttaccgcc gggctgtaac gccagggata aagcggcgag acgatcccgc cgccggccca
     5401 ggaggactca tgaccaagac agccagactc catcacgccg acccgccacc cgaccgctgc
     5461 aaggttgctc gctgtcaaaa gccccaacac cccaccacct actacgatga catccagcaa
     5521 aactaggtgc tccatatcga ctgggttaat tcagattttg tactacgctg aaggagcgtt
     5581 aaggacaaca ccaacaggaa ctcaggatga gaaccgactc aatagcagga tatagcctgc
     5641 tcgatgtact tattacactg gcacttattg gcaccctgat cggcattgga accccagcac
     5701 tctcgtcatg gagtgagcag cagaggctgc gcagcctgca aaccaatcta ttccacatga
     5761 ccaacaaagc ccgctacctg gcgatgacca gccagcaccg ggtcacgcta tgcccactat
     5821 cagaacaggg caaatgccag caaagctgga gcgggagcat aagcagcttt attgatagca
     5881 acggcaacag gcaacttgac gctggagaag agattttcag cacactctat gtgcccaaca
     5941 gcatcacgct aaattggagc ggcatgctgc ccaaaaactc aattcacttc agtaaccaag
     6001 gcgtcacctt cgttagcaac ggcacaatga gcctttgccc cacgaggccg gatagtcaca
     6061 gcggcacaat ggtaatcaac cgccaaggcc gtgtgaaaac atcgaaagac ggagcacatt
     6121 gcccttgagt agccaactac ctctcgacca ctcagcagcg ctaatccacc ggacatccca
     6181 agcgagcttg aaacgcgcgt ggcaactgac aatgatgcaa agcacatagt caggattccg
     6241 catgatgaag cagcaatctg gattcaccct tgttgagatg atggtcacca tcgcaatact
     6301 cgccatccta atgggtatag cggcgcctgc ggtcaccgat tttgcaatca agcaacgcgt
     6361 cagctcccag gcaaacgaaa tgatgctgtc tctggcattt gccagaagcg aagcagtaaa
     6421 actcaatcag gatgtcagag ttatccctcg gacgagtacc gccaacggct ggagtgatgg
     6481 ctggtgtgta ggccccgcca acattggcag cgactgtaac catgcagatg tcatacgagt
     6541 tttcacaggt gccggcgggg tgagtatcac cagctcaggc atcagcaacc cacctgcttt
     6601 agttttcctc cgtgatggaa ccgcctcgaa cagcgttgac gcacttctta aagtgtcatc
     6661 gccaaacctc gagccagcgg gcgacaccgc acgctgcatc aggctgacac gccctggccg
     6721 tgcagaagtt gaaaaagtcg cacgcgatgc tgcctgctga tcattaagga acgtatatga
     6781 gcccactttt atacaagcag cacggtgcca ccctgatcga agtattggtc gcgctgctga
     6841 tctttaccgt aggtttgctc ggcctggccg ccatgcagct tagctcgctg caaagcactg
     6901 ccgacagcgg ccagcgctcc cagtccgtat ggctgatgca agactttatc gagcgcatgc
     6961 gcaccaaccc agacggcact gcagcacagt atgccgctcc gcaaaattgc gccgccttac
     7021 cagcccgcat gtgcgccgac cactacaacc ccagcacggc tgcaaaggtc aacgccagtg
     7081 aatgcaacgc cacgcaaatg gctgcctttg atgcctggga agcacagtgc agctatgcag
     7141 cgattgcaac cttcaacaca attgatgctc gctttaatag ccgcgacttt atcacccctc
     7201 ccgtcgcagg caccctggtt gacgccatag ctgatggcaa caacctagat ctcaacttga
     7261 actggctcaa cagaagcaac ctgagcccta aaagccaagc acaagcagca gaaacagaaa
     7321 cagacggcac aacgacagta ctgagggttg aacgatgaac ccgcgcgaac atcgccagcg
     7381 ctcaagacag cgcggtttct ccctcgtcga actgctagta gcctttgtta tcagcatgtt
     7441 gttgctgaca ggtgttatcc aagtattttt tgcatcaaaa caaacctacg ccagtaacga
     7501 agccgcatca cgcttacagg agaacgggcg attcgcactg gagtttattg cacaaagcgc
     7561 acgccatgcg gggtatgtac agcctgataa caacgccgac aagccgagcc caattgccaa
     7621 gccaggtgat atttcttgcg attcaaataa caacgaagac gatgcctgta gcaatgacgg
     7681 cgacaacgct gctgctgata tatgggaacg atctgatcgt acaagcttta ttttccagcc
     7741 acgtcttgga gcggatgaca aacgccggga ctgcaatgga aatgaaatag ccaacaacaa
     7801 cgcacttatt cttaaccagt tcttcatagc accaccaagt gcaataacac cgccctacga
     7861 cccaaccctc cacccacccc tgaccctcaa ttgccgctcc ttaattttca attcaaacag
     7921 caaaggggct ttcataggcg tgcagtctcc acttatcgaa ggcattgacg cgttgcaaat
     7981 tcagtacggg cttagtgaaa aatccagtga tctaaacaat gtaagtcaat atatatcagc
     8041 cgaacgcgtt acagcattaa acaagtggag caatgtaacc gccatacgca ttgctgtatt
     8101 ggccaactca gtcgagcgca ctaaccctcc aacgcctgcc cgagcttatg ttctgctgga
     8161 cgcggctccc attgtcattg atgacggaag ggcccgccaa gtgttcacca ccactattaa
     8221 actacgcaat aatttttgag ggtcgcagca tgatgaacca caccccatcc cagcagactg
     8281 gcgccactct gatagttgcg ttggtgtttc tggttgtgct gaccatcgct ggcattactg
     8341 ccatgcagtt ctccacactg gaagagcgca tggccagcaa ctcgcaattt cgcaacgaaa
     8401 cctttcaaca agcccaaagc tcaatccagg caaaccttat agcactcagc gccaatgcca
     8461 ccggacgcga ccgactgctt agcgcactga atgccgcctc aaactcaagc gctgactctg
     8521 actgcgatga agactaccca ttcaacttat gcacgcaaaa gttggagcaa cttggccttc
     8581 aaccgactgc caagcaagct attgatgcca tagaagatca aacagcgaac ctcggattta
     8641 gcgtcatccg caatacagca ccggctaaag catgcgaaga cgacgtgtac ggcgcctcca
     8701 gcatcaccaa tttcgaatgc atcaactatg aactacaaat cagagaggaa ctgccaaacg
     8761 gcgcttactc tgaccaatcg caaggcctca cttttatgaa catcaaggcc aaaagttagg
     8821 agacgccagc atggttaagt caattacaag cctgatttta attctatcgt tcatttccac
     8881 aatcagcatc agccatgcag aaaccgccaa caatagccga acgactattg aaggggcgaa
     8941 tgtcaccatt cgcatgacca ataaccagcc ctatgcagag tttcaaccct gtgatgactg
     9001 cccatatcgt ctattgtcat ttaccccaac cgcgcaaatt tatttgaacg gcgaaaagac
     9061 tacagcaagc gcccttcaag acggacaaac aatggaaggg actgtattca tacagcaacg
     9121 ccccatagag tcgattattg aaatagttgc caattaacag gactaaagac atgaacattc
     9181 gctcactatc aattgtcctc ggcagctttt gctatatcag catgaccctg caaactgcac
     9241 ttgcagacga cactgaaata tacgtaagcc gaaaactccc tgaagaccag caggtacgcc
     9301 ccaacctgat gtttgtaatc gacaactctg gttctatgct aagtaacgtt cctggtacaa
     9361 cttgcgctgt ggctgaccga agcatccgca accgatgcga ggaagtactt ataagccaaa
     9421 catgcactcg ttacgactgg cgtgggcgtt gtgatcgctg gacgaacaac tatgaataca
     9481 gaaaaacacg cctgcaggtt gtaaaggacg tcaccaacga attagtcgaa gagctgcgcc
     9541 tgagcgacga tgtgaatatt gggctaatgc acttcgatgc agccccaaac acaagcaacg
     9601 aagaaggcgc catggttgca atcgcggcag gccgcgccag tacaacagcc aatagcttta
     9661 tcgcggagct taatcgcctc tatggtgcct ccaacacacc actgtcggaa agcttttatg
     9721 aagccgcact ttatatgaaa ggcgcaacgc caaaatttgg taatggcact aatgcggcct
     9781 acgaagatgg caatagctgg accaacccct ctaagcccag cgtcgccgct tcaaaatcag
     9841 gcagcacata caaaagcccc atccaatact cttgccaaaa aagcaatatc atattgctaa
     9901 ccgacggtga gcccacttat gacaactcag ccaacaataa catccgcacg ctgatcaaca
     9961 cctctggtgc agcaaacacc cagcacctgg caagcacatg tcgcgaccca ggcaccagca
    10021 gcaccagccg gagtggtgag tgcatgcccc acctcgcgga gcacctggcc aatcaggacg
    10081 ccagcagcag ccagactggc aaacaaacga tcaacacata caccattggc tttgcaacca
    10141 accagaccct actacaagag accgcaaccg ccggcaatgg ccgctatttc acaaccgata
    10201 acacctccgg cctggtcgac gctctcaaat cgattctggt agagatcctt gcggaaaact
    10261 ccacgttcgc aacacctagc gttgcagtta gtgcctataa caacctgggc tatcgcaatg
    10321 acctctacta cgccttgttc cgccccgcag aaggtgcgcg ctgggtgggt aacgtaaagc
    10381 gctacaaact aggggaagac agcacaagcg gcgacgccat tattgtggat aagaatggcc
    10441 aaccggctgt tgatgaaacc acaggcttct ttagggaaaa cgcttcaagt tattggtcct
    10501 tccaagacgg cagagatgtc gccaaaggtg gcgtagccgg catgctcggc aaccctgccg
    10561 atcgaaagat ttatacatgg acagcagccg atcgatctcc aactgcaggt gctggtgtga
    10621 ccgggagcgt gctaatcaat accagcgccc atgcactgac aactgctaac gccgctatta
    10681 ccactgcgct gatgggtgtc agcaccgccg cagccaaaga caacgccgtc aaatggggcc
    10741 gtggcttgga ccctgtaaca aaccaagccc gccaacaact tgccgatgtg ctgcataacg
    10801 aacctcgcct ggtcgcctat aaaaccgatg aagacttggt gcgtgtcgaa aatatcaaga
    10861 atggcactgc gtcgccgtcg acttccccgg aagagcttta tatgttcttc ggcaccaacg
    10921 aaggctttat tcatgcagtc agccctcaaa caggggaaga aaagttctca tttattccca
    10981 aagaactact accaaatctg aacgcctact acaccgaccc aaaaggctcc gatgcaaaac
    11041 gctatggtat ggacgggcag atcaacctat gggttgagta cggcgccatc aatacaacga
    11101 ccaaatcccg cagcattacc aaaaactacc tttacgcagg tatgcgccgt ggcggccgca
    11161 actactacgc actggacgta ggcaccatca catcacccgc tctcaaatgg gttatcaagg
    11221 ggggcacaac ccctggcttc gaaaaactgg gacaaacctg gtccacgcct aagttggcgg
    11281 atatcaatgt caacagtaca aaaaccaagg tcctgatttt cacgggcggc tatgaccctc
    11341 agcaggacga cgacggcacg aacacccccc tagcaaacga caattacggc aacagcctgt
    11401 atatcgtcaa tgccgaaacc ggccagctga tctggcgtgc aggccataca tcggagactg
    11461 gggctaacct gcagctttcc aacatgaccc atagcatgcc tgccgacccg gtgatcatcg
    11521 acatcaacag cgatggcctg gcagacatta tttatgcaac cgatacccgc gcccaggtat
    11581 tccgctttga catcaacaat gccaacgccg gggccagcga ccttggcgta ggtgggcgta
    11641 ttgccagcct aggcggcgct aacgcgctga ataatcgcag gctgtttaac acgcctgacg
    11701 tcgcattggt gcgcgaacgt ggcagtaaga cctacttcac catatctcta ggctccggct
    11761 accgagctca cccactcaac gaagacacga ttgatcgatt ctatgtgctg cgtgatgaaa
    11821 atgttttcaa caagcctaca agctatgcat acgctgccac tgcgagcacg ccagacaagc
    11881 cgataactga gagtgatcta gtcgacgtat ccagcgttga tcttacagac gcgcaagctg
    11941 cagaagttct cgaagaaatt gctgagctgg aagccaaaat caacgcgttg aaccagagcg
    12001 tagccgacac tagggatgta ttcaccgcgt tcaagaacag ctcgggtttc actgacaaat
    12061 ataacgccat gctacaggcc aatagcagcg caaatgatcg gcaggtggcc atcgattcgc
    12121 taatggcgtc cgacccttac ttgcttgccc acgcgcctga aagccttgag caaagcaagc
    12181 ttcagcaagg cctgcttgca agccaacaga cactacaggc attccaacaa gcccgcactg
    12241 cagcggatag tgatttagca gccaaggaag cggcttacaa cagcgcctca gctgataagg
    12301 caactgcagt agccgccaga gaagccgcgc aaaccgctta tgacgcagat aacagtccag
    12361 aaaaccaaac aaatctggac aatgccactg cagcagaaac agcagcaatt aacgcgtacg
    12421 ctagcgcaag tgcagcagca caagctgcct tcgacaaaaa gaacacgctg cacactcagg
    12481 ccgagtcact cgctggactc tacagcacac tgaccaactt gcagagtgaa ctgtcttcgc
    12541 gccatcagca gatcctcgac aaagagcaag ccatcatcgc ggcaaaggct acggctgcag
    12601 acgaaacagc tattgcctcg cttgagacag agctagcggg cctgaacagc agttatgcca
    12661 gcgatccgtt aacaacagag cgtgacgctc taaatgccgg tgccaccagc actgtactag
    12721 aagatgcatt ggctcagctc aataccgcca taacgggtgg tgacacctca acagttcaga
    12781 ccgcactgga taatctggaa accctagccg gtccacctac aggtccagcc cagagcattg
    12841 aagatctttt aagccgtgat gaggcagcga aaaacacaga gcttgcgaac aatgcagaaa
    12901 accaagccag taacgtcgca ctgctcgata cgctggacag ccagcgactt gccttcgcag
    12961 ggcaagcatc cacagcgcaa gccgaagcca atgccatagc caatgcggct tatgatgaag
    13021 gctctgcatt actgaccgca gcacagctgg ctgccgccaa agcgctgtac ggaagtgatc
    13081 tgaccatatt cgaggcttac cagtacctaa tagatcaggc acaactggca accctggacg
    13141 aagataaagg cctccctgca ctgcgtgaaa gtatcaactc gctttatgcc caactgacgc
    13201 caggcaacaa ctacgtgcct aataaaacac tactggccaa cagtaaaggc tggttcctgc
    13261 gcctacccaa aggtgaaaag gtgctctcgt cgcccctgat cttccgcgga gctgttttct
    13321 tcaacacatt tagcccgcgc ggcgagaccg tcaccacctg cggccctgat gtggggcgcg
    13381 gtcgcgccta cgcacttagc ctgaaggatg ccagcgccat tctttcacgg acaggagcaa
    13441 ctccgggaag tgaaacgccc attcgcagct acgcgctgac ccgctcaggc attccaccag
    13501 ccccatccgt tgtcttcagt gacaacggac cacccaaggt catcatcggc accgaagtgt
    13561 taaatgacag agacccttcc gacaacagcg cagcggaatg cactgcaggt gagaagtgcc
    13621 caataaaatg cacaggggat gttgggtact gcccaaaaaa tccgcagact attgagaaaa
    13681 cctattggag agaaaactga tgcgacagca gcgcggcttt acgctgatcg agttgatgat
    13741 tgtgatcgca atcatcggca tactcgcggc aattgcctac ccgagctacc aagaatatca
    13801 actgaagtca ggccgctctg atggccacgc aaagttgatg caggttatgc aggctcagga
    13861 gcgcttctac tcgcaaaacc agagctatac cgacaacctt ggagccactg ctgccggcaa
    13921 tcctggtctt gcgtttccgg gtacagccga ggacgccccg gtaattagtg acgagcagcg
    13981 ttacagcatt gctgctgaag tgtgcgctgt tggcacgcca ctaactcgtt gcgtcaggct
    14041 ggtggctacg gctgtaggcc cacaagttgc tgatgcccaa tgtggcaatc tcagcctgga
    14101 tagccgaggc gttaaaggtg agtctggaac aggtacagtt caagcctgct ggtagtaagc
    14161 gcagccagta aatacaaaaa agccgactcc atgagtcggc ttttcattta agtagcctaa
    14221 ggcaagtgat cacactggct tcattcgcaa ctctttcggc atcgagaacg tcacattctc
    14281 cggccgccct agcagctctt gcgcacccgt tgcgccccac tcgctcaatt gctgaataac
    14341 gccctgcacc agcacctctg gtgcagacgc gcccgctgtg atgccaatac ccgccacacc
    14401 ggcaaaccac tcttgcttga ggtcttccgc cccatcgatg aggtaggcag gcgtgcccat
    14461 gcgctcggcc agttcccgca ggcggttgga gttggagctg ttggggctgc ctaccaccaa
    14521 tagtacgtcg cattcagcgg ccagttgctt gaccgcgtcc tggcggttct gggtggcgta
    14581 gcagatatca tccttgcgcg ggccgccgat ggcggggaat ttgctgcgca gggcgtcgat
    14641 caccttgctg gtgtcgtcca tcgacaaggt ggtttgagta acgaaagcca gcgcctcagg
    14701 gttgcgcacc tgcagttcgg ctacgtccgt ttcgtcctcc accaagtaga tggcgccgcc
    14761 gttgctggcg tcgtattgac ccatggtgcc ttcgacttcc gggtggcctt cgtggccgat
    14821 gaggatgcat tcgcgcccct cgcggctgta gcgcaccact tccatatgca ccttggttac
    14881 cagcgggcag gtggcatcaa atatcttcaa accacggcgt tctgcttccg cgcgtacagc
    14941 ctgagaaacg ccatgggcgc tgaagataac gatgacatcg tcaggaattt gatccagctc
    15001 ttcgacgaag atcgcgccac gcgaacgaag gtcttcgacc acaaatttgt tgtgcaccac
    15061 ttcatgacga acgtagatcg gtggaccgaa gacttccaga gcgcgattga cgatctcaat
    15121 ggcgcggtcg acgccggcac agaagccacg gggattggcg agtttgatat gcatggcggt
    15181 atctcgacgg tagaccctgc gggctagtgt acggcgcagc cgcacgcact gggaaagcaa
    15241 tctgtgtaag ccgccgtgat cgcgaggatc acgcgggaac acgcaagacg aaagccgctc
    15301 ccacaggagc gagctaaacc ttagagcgct ttgacctcaa taatctccac ctcaaagctc
    15361 aacgccttgc cggccagtgg atgattgaag tcgatggtca cctggttata gtcaaaggct
    15421 ttgaccacac ccggca
//
LOCUS       DASRUM010000022        24183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2354158, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000022 DASRUM010000000
VERSION     DASRUM010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 24183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24183
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2354158"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="VFF22_01500"
     CDS             <1..175
                     /locus_tag="VFF22_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15529.1"
                     /translation="GNLSLSVIDRTSTRLLGRTQLSSTIYSDPVQFADVLKQSREQLS
                     QQGFTLEPWSMPE"
     gene            373..588
                     /locus_tag="VFF22_01505"
     CDS             373..588
                     /locus_tag="VFF22_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008011009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HZX15530.1"
                     /translation="MNEDENKPLTLWEMLQSVLSAAIGVQSGKNRSRDFSRGKPSHFI
                     ILGVLFTAVFVLVIFGVVKLVLHLAGV"
     gene            complement(590..1699)
                     /locus_tag="VFF22_01510"
     CDS             complement(590..1699)
                     /locus_tag="VFF22_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX15531.1"
                     /translation="MFRSVFTVIGAVCVLAFAHVVQAAEDVRAAGKASATVVFLNPGF
                     SSEPFWVGYSDFMQAAADDLGMHLQIIYGERNPPQLLAKARSVLARDKQPDYLIFVNE
                     MYTAPELLRLFADSPIKLFTLHSTLTPEQQQRIGGPREQYRNWIGSLIPNDEQAGYWM
                     TKALIAKLQGKPGSLLAFGGVRFTPSSSLREDGLRRALQEHPEIKLQQLLYGEWRRQR
                     AYEQAQLMLPRYPEVQLVWSANDEMAFGVMRAAQELGKQPGKDIYLAALNNSDEVLQA
                     RIDGKVSALVGGHFTLGGWAMVMLHDYHAGLDFAARGGKERVDQLFSLLDVKQAAHLR
                     QRLKVPGYGLNFRQFSAVYHPQIKDYGFSIKPLLQ"
     gene            1848..2414
                     /gene="pnuC"
                     /locus_tag="VFF22_01515"
     CDS             1848..2414
                     /gene="pnuC"
                     /locus_tag="VFF22_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017906956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HZX15532.1"
                     /translation="MSSLEIIASALGVWAVWLTVRQNRWCWPLGLVMVLMYAWIFYDG
                     KLYSNMLLQGVYAVLQGYGWWQWTSGGNSHTGVQVSRLSHQGILISLAIGASGAVILG
                     YLMATFTDAAAPWQDAALSALSLVAQVWMAQKRVECWPLWIVLDLLFVALFLQQGLYP
                     TAALYGLFTLLAVNGWLTWRRDRALVSA"
     gene            2425..2976
                     /locus_tag="VFF22_01520"
     CDS             2425..2976
                     /locus_tag="VFF22_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZX15533.1"
                     /translation="MKVLVLTGPESSGKSWLAGHIQAAFGGQLVGEYVRHFIDREQRD
                     TCYADISTIAQGQLAWEDQARAARPELLILDTHLLSNMLWSQELFGDCPAWIEQALLE
                     RDYHLHLLLNPVDVPWVDDGQRCQPELVQRMAFYQQCEHWLRLHQQPLQCIQGDWAQR
                     RELTLATVARWLSSAQQHAGPGQ"
     gene            complement(2944..3411)
                     /locus_tag="VFF22_01525"
     CDS             complement(2944..3411)
                     /locus_tag="VFF22_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HZX15534.1"
                     /translation="MHPFDAPKPPKLALLLGYAGLVPFVTGALGIWVTPEGWRVVVLA
                     ALLDYAAVILAFMGAIHWGLAMRAEDASEPAQIQLGLSVVPPLLGWLAISSGMPISLA
                     LPLCLLAFGGLYAADLRAVKLGLAPQWYPSLRRPLTIVVCLSLLLAWASVLLR"
     gene            complement(3437..3574)
                     /locus_tag="VFF22_01530"
     CDS             complement(3437..3574)
                     /locus_tag="VFF22_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15535.1"
                     /translation="MDTSLWRFLFVVFMPIAAADGKRPLQQNSGIMLLLGEKKALQCD
                     L"
     gene            3573..5447
                     /locus_tag="VFF22_01535"
                     /pseudo
     CDS             3573..5447
                     /locus_tag="VFF22_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492404.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme-cytochrome C peroxidase"
     gene            5501..6634
                     /locus_tag="VFF22_01540"
     CDS             5501..6634
                     /locus_tag="VFF22_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family protein"
                     /protein_id="HZX15536.1"
                     /translation="MFKRFWLWLGRVLGKLLVLVLVIGIGGWAIGAAYYGWKFSGPVS
                     TEEQIPADEAALTQGIIEDAIRVVEQHRDNTRVLRDAHAKAHGCMKAEVTVSADIDSS
                     LQRGVLSEPGKTWQAWMRLSNGNAYPQFDRARDARGMAIKLLDVPGEKLTQSPQHASE
                     QDFVMFNHPAFFVRDVAEYKSNFAAQADGKKAMAFFPSWNPSTWEIRHLIIALKTLSP
                     APETPVATTYNSIAPFKLGEHNIKYRVIPQPEACPEYQLPEQNQDLPNFLRNALYQQL
                     SLDRVPACFALQVQRQNAEYYMPIEDPSVEWSEAISPFETVATIKVPAQDFDSREQNL
                     FCDNLSFNPWHALPEHRPIGGINRLRKAVYEAVSIYRLERNQP"
     gene            6740..7096
                     /gene="queD"
                     /locus_tag="VFF22_01545"
     CDS             6740..7096
                     /gene="queD"
                     /locus_tag="VFF22_01545"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HZX15537.1"
                     /translation="MELFKEFMFEAAHRLPHVPEGHKCGRLHGHSFKVAVYIEGEVDP
                     YTGWIRDFSEIKAIFKPLYEQLDHNYLNDIPGLENPTSEVLAKWIWQQLKPLLPELSR
                     IRIHETCTSGCEYRGD"
     gene            complement(7135..9534)
                     /gene="ligA"
                     /locus_tag="VFF22_01550"
     CDS             complement(7135..9534)
                     /gene="ligA"
                     /locus_tag="VFF22_01550"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZX15538.1"
                     /translation="MTDASTAAQRIVQLRAELDGHNYRYYVLDEPSIPDAEYDRLFRE
                     LQALEAEHPQLVTAESPTQRVGGEAIAAFGEVRHEIPMLSLGNAFEEGDLLAFDRSVQ
                     SGLGLAGDLFGGGADVEYSCEPKLDGLAVSLRYENGQLVRGATRGDGSTGEDISSNVR
                     TIRNVPLKLQGEGWPQVLEVRGEVYMPKAGFDELNARQAESGGKTFANPRNAAAGSLR
                     QLDPRITASRPLEFCCYGVGQVSGELATTQVGMLEQLKAWGIPISRELKLAKGVQACL
                     DYYRDIGERRMSLAYDIDGVVFKVNNIEDQQQLGFRARTPHWAIAHKFPAQEELTELL
                     SVEFQVGRTGAVTPVARLKPVKVAGVMVANATLHNMDEVARLGLMIGDMVIIRRAGDV
                     IPQVMQVVLDQRPDWSEEELLARQISIPTECPVCGSHVERTQLVKRSKGKETISEGSV
                     YRCVGRLACGAQLKQAIIHFVSRRAMDIEGLGDKTIEQLVDEQLIASPADLYKLTFEQ
                     IINLEGFAEVSSNKLLQAIADSKQPTLARFIYALGIPDVGEETAKVLARSLGSLRRIR
                     KAYSEVLIYLPDVGLEVAHEIHNFFSDAHNRKVIAQLMRGSVAPLMDHGISPEFAACA
                     SLAGFIDKLNIPSVGPTGAEKLAVKQNSLEKIIAADGIDLRQALNEKPAKAVREFFEV
                     AANVERARAIEAQLKAFGMHWLSEKKVIEGLPLAGQTWVLTGSLESFSRDKAKAELEK
                     LGAKVAGSVSAKTSVVVAGPGAGSKLTKARELGVQDIDEAEFKQRLRAFGVELGGVRS
                     C"
     gene            complement(9596..10420)
                     /gene="zipA"
                     /locus_tag="VFF22_01555"
     CDS             complement(9596..10420)
                     /gene="zipA"
                     /locus_tag="VFF22_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HZX15539.1"
                     /translation="MEFGLREWLIVIGIIVIAGILFDGWRRMRGGKGKLKFKLDRSFS
                     NLPDDDSDPDLLSPPRVIDRSHEPAFDEEDLPSLSARELNRRRSGEPQQGDLNLGLEE
                     PVPTLLNPVDDDVVKPTSQRKESSKELPPVEEVLVINVIARDEEGFKGPALLQNILES
                     GLRFGEMDIFHRHESMAGNGEVLFSMANALKPGTFDLDDIEGFSTRAVSFFLGLPGPR
                     HPKQAFDVMVAAARKLAHELGGELKDDQRSVMTAQTIEHYRQRIVEYERKQLTNKR"
     gene            complement(10603..14091)
                     /gene="smc"
                     /locus_tag="VFF22_01560"
     CDS             complement(10603..14091)
                     /gene="smc"
                     /locus_tag="VFF22_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HZX15540.1"
                     /translation="MRLKCIKLAGFKSFVDPTTVTFPSNMAAVVGPNGCGKSNIIDAV
                     RWVMGESSAKNLRGESMTDVIFNGSNTRKPVTQASIELIFDNSDGTLTGEYAGYNEIS
                     IRRRVTRDSQNTYFLNGTKCRRRDITDIFLGTGLGPRSYSIIEQGMISKLIEAKPEDL
                     RNFIEEAAGISKYKERRRETENRIRRTHENLARLTDLREELERQLDRLHRQAQSAEKY
                     QEYKAEERQLKAQLTALRWQALNEQVGSREAVISNQEVSFEALVAEQRNADASIERLR
                     DGHHELSERFNLVQGRFYSVGGDIARIEQSIQHGQQRLRQLQDDLNEAERARLETESH
                     LGHDRTLLATLGEELAMLEPEQELTAAAAEESAAGLEESETAMHGWQEQWDSFNQRSA
                     EPRRQAEVQQSRIAQLEQSLERLAERQRRLSDELQQLAADPEDAAIIELGEQIAAGEI
                     SLEDLQLEEEQQAERLQQLRNDLQHSGQAQQQAQGELQRLNGRLASLEALQQAALDPG
                     KGAGEWLREQQLSERPRLAEGLRVEAGWELAVETVLGADLQAVLLDDFQGLDFAGLTQ
                     GDLRLASPSKGGARVAGSLLDKVEASIDLAPWLAKVKAVQSLDEALAARLSLAEGESL
                     ISRDGYWVGRHFLRVRRASEAESGVLARGQEIERLSLERDKREAALALLDERLLHLRE
                     NQRVQEEQREQQRRQAQDQARRLGELKAQLSAGQAKVEQLILRRTRLDSELLELGEQR
                     AIEHEQLGESRLQLQDALNAMATDNEQRESLLARRDSLRERLDRVRQEARQHKDHAHQ
                     LAVRLGSLKAQHDSTRQALERLELQAERLHEKREQLNLNLEEGEAPLEELRMKLEEQL
                     DKRMAVEDELKLARLALEDADRELRDAEKRRTQAEQQAQLLRGQLEQQRMDWQSLTVR
                     RKALQDQLLEDNYDLHGVLASLPAEASEKAWEEELERLAARIQRLGPINLAAIDEYQQ
                     QSERKRYLDAQNADLVEALDTLENVIRKIDKETRNRFKETFDQINGGLQALFPKVFGG
                     GNAYLELTGEDLLDTGVTIMARPPGKKNSTIHLLSGGEKALTALALVFAIFKLNPAPF
                     CMLDEVDAPLDDANVGRYARLVKEMSETVQFIYITHNKIAMEMADQLMGVTMHEPGCS
                     RLVAVDVEEALALVES"
     gene            complement(14104..14763)
                     /locus_tag="VFF22_01565"
     CDS             complement(14104..14763)
                     /locus_tag="VFF22_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016979284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZX15541.1"
                     /translation="MTFKAPDSLAEQIAHHLAERIIRGELKARERIQEQKVTQALNVS
                     RGSVREALLILERRHLIVIPPRRGAQVAELTPHNVKSLYTLVIELYILLGKAVADSWQ
                     VEADLGPFLVIQQRLQDSLQREDINGFVESSFDIMRAAFPFSNNPYLQETVENLLPAI
                     SRTYHLALERRKGEMGQFLNTFAALLQAIIVRDHQAVREVLLGYGEHNCQLVLAALAD
                     A"
     gene            15000..16139
                     /locus_tag="VFF22_01570"
     CDS             15000..16139
                     /locus_tag="VFF22_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HZX15542.1"
                     /translation="MFERLPSNWMLVIKSIGYWLWLIPVLGIPLSYYWSLNSQYANAW
                     AWLVISVVFGLIPLLDFIVGRDPANPDEAVQVPQMEQQGYYRLLSLATVPFLLGMLGW
                     AGWVFVSYEAWSWVGQLGWVLSVGTVTGAIGITVAHELIHKDPKLEQNAGGLLLAAVC
                     YGGFKIEHVRGHHVHVSTPEDASSSRYGQSLYAFLPHAYKHNFLNAFALEAERLKRKN
                     LPALHWRNELLAWYAISALFLLGFSLAFGWLGALFFIGQSIVAFTLLEIVNYVEHYGL
                     HRRKLENGRYERTNPTHSWNSNFLLTNLFLFHLQRHSDHHAYAKRRYQVLRHFDDSPQ
                     LPNGYAGMIVLALFPPLWRAVMDPRVRAYYAGEEHQLTDSQLTHS"
     gene            16449..17888
                     /gene="xdhA"
                     /locus_tag="VFF22_01575"
     CDS             16449..17888
                     /gene="xdhA"
                     /locus_tag="VFF22_01575"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HZX15543.1"
                     /translation="MIQFLLNRELRTEHALDPNITVLNYLREHLGKSGTKEGCASGDC
                     GACTVVVGELIGERVRYRTLNSCLTFVSSLHGKQLITVEDLKHQGQLHSVQQAMVDCH
                     GSQCGFCTPGFVMSLFALQKNSNGFDKADTMEALAGNLCRCTGYRPIIDAAEQACCQK
                     QPDQFDAAEAQTVAQLKAIAPQDTAELNSGDKRCLLPLTVADLADIYTANPEARLLAG
                     GTDLALEVTQFHRELPVMIYVGHIEAMKRVDVTAESIEIGAATALSDCYAALAQDYPD
                     FGELLHRFASLQIRNQGTLGGNIGNASPIGDAPPLLIALGAQIVLRKGNASRTLPIED
                     YFLDYKVTARQEAEFIEKIIVPRHQANQAFRAYKVSKRLDDDISAVCAAFNLVVEDGV
                     VQSARIAFGGMAAIPKRAAACEAALQGSAWYPGVIERACAALAEDFTPLSDFRASKEY
                     RLLIAQNLLRKFFLELQAPEVETRVTAYV"
     gene            17881..20274
                     /gene="xdhB"
                     /locus_tag="VFF22_01580"
     CDS             17881..20274
                     /gene="xdhB"
                     /locus_tag="VFF22_01580"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HZX15544.1"
                     /translation="MSNHIQHKTQAELAELFRADITTGVGKSVKHESADKHVSGEAIY
                     VDDRLEFPNQLHVYARMSDRAHARIVSIDTAPCYAIPGVAIAITSKDVPGQLDIGPVV
                     AGDPLLADGKVEYVGQVVIAVGADSLETARKAAMAAIIEYEDLEPVLDVVDALRKKHF
                     VLDSHQHKIGNSAAELATAPRRLQGSLHIGGQEHFYLETQISSVMPTEDGGMIVYTST
                     QNATEVQKLVAEVLGVPMHKIVIDMRRMGGGFGGKETQAAGPACLCAVIAHLTGRPTK
                     MRLPRVEDMQMTGKRHPFYVEYDVGFDDNGLLHGIEIELAGNCGYSPDLSGSIVDRAM
                     FHSDNAYFLGNATINGHRCKTNTASNTAYRGFGGPQGMVAIEEIMDAVARSLGKDPLE
                     VRKTNYYGKTERNVTHYPQTVEHNVIHEMTAELEASSDYAKRREEIRAFNAKSPVLKK
                     GLALTPVKFGISFTATFLNQAGALIHIYTDGSIHLNHGGTEMGQGLNTKVAQIVAEVL
                     QVDISRIQITATNTDKVPNTSPTAASSGADLNGKAAQNAAETLKQRLVDFLVREYKVT
                     PEDVEFRNSQVRVRDLFLSFEEVIQKAYFGQVSLSSTGFYRTPKIFYDRDKAAGRPFY
                     YFAYGAACSEVIVDTLTGEYKMLRTDILHDVGASLNPAIDIGQVEGAFVQGMGWLTME
                     ELVWNGKGKLMTNGPASYKIPAIADMPLDLRVKLVENRKNPEDTVFHSKAVGEPPFML
                     GIAVWCAIKDAVASLADYKVQPNIDAPATPERVLWGVEQMKKLQQPAKAAAAEVEPA"
     gene            20456..21292
                     /gene="xdhC"
                     /locus_tag="VFF22_01585"
     CDS             20456..21292
                     /gene="xdhC"
                     /locus_tag="VFF22_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017246470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HZX15545.1"
                     /translation="MSWISALADLQQRGEPCVLVTIIEERGSTPRNAGSKMVVTADRI
                     FETIGGGHLEFKAMELAREMLQSRSQDTRLERFSLGASLGQCCGGATVLLFEPMGQPQ
                     AQIAVFGAGHVGRALVPLLASLPCKVRWIDSRENEFPGQIPAGVDKVVNEEVIDEIER
                     MPAGSYFIVMTHNHQLDLELTAEILKRNDFAYYGLIGSLTKRAKFEHRLRDRGFDPAL
                     VARMRCPMGLAEVKGKLPVEIAVSIAGEVIATYNAAFGQDVKKGETSVAKLLPASRRA
                     TP"
     gene            21368..22672
                     /gene="guaD"
                     /locus_tag="VFF22_01590"
     CDS             21368..22672
                     /gene="guaD"
                     /locus_tag="VFF22_01590"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HZX15546.1"
                     /translation="MTNTLKAYRAAILHNIADPAVVGVEQSYEYFEDGVLVIENGQVA
                     RVGHAAELLPTLKGVEITEYRDALITPGFIDTHIHYPQTGMIASYGEQLLDWLNTYTF
                     PTEQQFADKAHAADVAGIFLKELLRNGTTTALVFGTVHKQSVDAFFEAAEALNLRMIA
                     GKVLMDRNAPDYLTDTPESGYADSKELIERWHGKGRLHYAVTPRFAPTSTPEQLTLAG
                     KLLGEYPDLYMHTHLSENRKEIEWVKALFPERKGYLDVYDHYKLIGARSVFAHGVHLC
                     DDECKRLAETGSAVAFCPTSNLFLGSGLFDLNKLEQHGVRVGLGTDVGAGTSFSQLQS
                     LNEAYKVMQLQGKKLDPFKSLYLATLGGAHALYLDDKLGNFESGKDADFVVLDYNATP
                     LISYRMQQAKTLEERLFALTMLGDDRAVKETFAAGQSVHRRD"
     gene            complement(22763..23521)
                     /locus_tag="VFF22_01595"
     CDS             complement(22763..23521)
                     /locus_tag="VFF22_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZX15547.1"
                     /translation="MNEQLQPLKKQTREGKSTRSGTQDDVVYAHIFDAILEQRLAPGT
                     KLSEEALGEIFGVSRTIIRRALSRLAHEGVVLLRPNRGAVVASPGAEEARQIFYVRRM
                     IERAITELACKNAKPEQIAALRQMVIDERDCFARGDRGMGIRLSGEFHLKLAEAAGNL
                     TLLGFLRSLISQTSLIIALYESNSRTHCSDAEHDQVIDAIEAGNVDVAVDLMMRHMDS
                     VDAKLNLDGETASDDLHAVFSHLLPSGKKKAASR"
BASE COUNT         5061 a         7663 c         6651 g         4808 t
ORIGIN      
        1 tggtaaccta agcctgagtg tgatcgaccg taccagcact cgcctgctcg gccgcaccca
       61 actgtccagc accatctaca gcgacccggt gcaatttgcc gacgtgctca agcaatcccg
      121 tgagcagcta agtcagcaag gtttcaccct ggaaccatgg agcatgcccg agtaatcggc
      181 tgctgcgaca agggctctga gttgccaatc ggcccctctt gtcagagccc ttcttccctt
      241 ctttgcccgt atcgaactta ccccagccct tgcactcatt tttccggctg agctttgctg
      301 cgtctgcaca taagcgctta tgctcaaagc ctgatactta gcaaaaccag tcagagcaaa
      361 gcaggggtgg ctatgaacga agacgaaaac aaaccactga cgctgtggga gatgctacag
      421 agcgtgctga gcgcagcaat aggcgtgcaa agcgggaaga accgcagccg cgatttctca
      481 cggggcaagc ccagtcactt catcattctc ggtgtgttgt tcacagccgt gtttgtgctg
      541 gtgatcttcg gtgtggtcaa gctggtgctg catttggcag gcgtctgact tactgcaaca
      601 acggtttgat ggaaaagccg tagtctttga tctgcgggtg gtacaccgcg ctgaactggc
      661 ggaaattcag gccgtagcct ggcaccttga ggcgttgacg caagtgcgcg gcctgtttga
      721 catccagcag gctgaacagc tgatcgaccc gttccttgcc gccgcgcgca gcaaaatcca
      781 acccggcatg gtagtcatgc agcatcacca tggcccagcc gcccagggtg aaatgcccac
      841 cgaccagcgc gctgactttg ccgtcgatac gtgcctgtag tacctcatcc gaattattca
      901 gggccgccag gtaaatatcc ttgccgggtt gtttgcccaa ctcctgcgcg gcccgcatca
      961 ccccaaaggc catttcatca ttggctgacc acactagctg cacctcggga tagcgcggca
     1021 gcatcaattg cgcctgttca taagcgcgct ggcgtctcca ctcgccatac agcaactgct
     1081 gtaacttgat ctccggatgt tcctgcaagg cgcggcgcag accgtcttcg cgcagactag
     1141 aagatggggt gaaacgaact ccgccaaacg ccagcaggct tcccggtttg ccttgcagct
     1201 tggcgatcag ggctttagtc atccagtaac cggcttgctc atcattgggg atcaggctgc
     1261 caatccagtt gcgatactgc tcacgcggac cgccaatgcg ctgttgctgc tcaggtgtga
     1321 gggtgctgtg cagggtaaac agcttgatcg ggctatcggc aaatagcctg agcaattccg
     1381 gcgcggtata catttcgttg acgaatatca ggtagtccgg ctgtttgtcg cgggccagca
     1441 cgctgcgggc ttttgccagc agctgcggcg gattgcgttc gccatagata atctgcaaat
     1501 gcatgcccag atcatcagcg gccgcctgca taaagtcgct gtaaccgacc cagaacggct
     1561 cactggagaa gcccgggttg agaaaaacca ccgtcgctga agccttgcct gccgcacgca
     1621 cgtcttccgc cgcctggacg acatgggcaa aagccaatac gcaaactgca ccaataacag
     1681 taaaaacgga cctaaacacg ccatgcctcc tcaacgggac gcagagtata tcggcaaagc
     1741 catagccatt agccacctag ccaacatctg cggccgtgca gccaagacgg cgcacagcgc
     1801 aatcgctaca atcgccagcc attcccgccc tctaacaccg gtgttctatg tcgtctctgg
     1861 aaatcatcgc ttcagccctt ggtgtatggg ccgtctggct gaccgtgcgg cagaaccgct
     1921 ggtgctggcc actgggcttg gtcatggtgc tgatgtacgc ctggatcttc tatgacggca
     1981 agttgtactc gaacatgctg ctgcaaggcg tttatgccgt actgcaaggc tacggctggt
     2041 ggcaatggac gagcggcggc aacagccata ccggcgtgca ggtcagtcgg cttagccatc
     2101 aaggcatatt gatcagctta gccatcggtg ccagtggcgc agttattctc ggttacctga
     2161 tggccacctt caccgatgct gccgcgccct ggcaggatgc agcgctgagc gcgctcagcc
     2221 tggtcgccca ggtgtggatg gcgcagaaac gggttgaatg ctggccactg tggatcgttc
     2281 ttgatctgtt gttcgtggcg ctgtttctgc aacaaggcct gtacccgacg gcagcactct
     2341 atgggctgtt taccctgctg gcagtcaacg gctggctgac ctggcgccgt gatcgcgccc
     2401 tggtcagcgc atgaaggccc agacatgaaa gtactggtgc tcaccggacc ggagtccagc
     2461 ggtaaaagct ggttggccgg gcatatccaa gcggcgtttg gcgggcagct ggtgggcgag
     2521 tatgtgcgcc actttatcga ccgcgagcag cgcgacacct gctacgccga tatcagcacc
     2581 atcgcccagg gtcaattggc ctgggaagac caggccagag cggcgcgccc cgagctgctg
     2641 attctcgaca cccacttgct gagcaatatg ctttggagcc aggagctgtt tggcgactgc
     2701 cccgcctgga tcgaacaggc cctgctcgaa cgcgattacc acctgcacct gctgctcaat
     2761 cccgtcgatg tgccctgggt cgacgatggc cagcgctgcc agccagaatt agtccagcgc
     2821 atggcgtttt accagcagtg cgagcactgg ctgcggctcc accaacagcc gctgcagtgc
     2881 attcaaggcg actgggcgca gcgccgtgag ctaaccctgg cgacagtggc gcgctggctc
     2941 agttcagcgc agcagcacgc tggcccaggc cagtagcaag ctcaggcaga cgacaatggt
     3001 caacggccgg cgcaggctgg gataccactg cggcgccagg cccagtttca ccgcccgcaa
     3061 atcagccgca tacaaaccgc caaacgccaa caagcacaga ggcaaggcca ggctgatcgg
     3121 cataccactg ctgattgcca gccagcccaa caatggcggc accaccgata accccagctg
     3181 tatctgcgcc ggctcgctgg catcctctgc ccgcatggcc aagccccagt gaatcgcccc
     3241 cataaaggcc aggatgaccg ctgcgtaatc cagcaaggcc gccaaaacca ccactcgcca
     3301 gccctctggc gtgacccaga ttcccaatgc cccggttacg aaaggcacca ggccggcata
     3361 gcccagcagc aacgccaatt taggcggctt cggcgcgtca aatggatgca tgcactcact
     3421 cctgttggtt aatccgtcat aaatcgcact gtaaagcctt cttttcgcct aacagaagca
     3481 taattccgct gttttgctgt aatggccgct tgccatcggc tgcggcaata ggcataaaaa
     3541 caacaaataa aaaacgccac aaggaagtat ccatgcgcat cctccgtcgg gccctgtatg
     3601 ggcttgtttt gttgctggtt gtggggctgc tggttgccct atatttcatc gcatacccca
     3661 acctgccggt ctatcaacag cccgacaaac tgcactacct cgatcagtgg tccgcagaag
     3721 aacgccagac ctactactac actccccagg gtacacaggt gaaaggcctg cgctacgaat
     3781 ggttcggcgc tctggagcta ccattcagca gcagcaagtt cgccaccccc gactaccttg
     3841 cgcgcttcgg tttcctcgtc gaccccgcgc aacagccctc cccgctaaac cccggtaacc
     3901 tgccggtcgg ctttgcacgg catgccgatg ctgagagcgg tgagcagtat ctggatatca
     3961 gctgcgcagc ctgccacacc ggcgagctgc gttataaagg ccaggccgta cgcatcgacg
     4021 gcggcgccgc gctacattcg ctggcctcca ccgtgcccac catacgcggc ggcagctttg
     4081 gccaggcact cggcatgagc atggccttca cctattacaa cccactgaaa ttccgccgct
     4141 tcgccaaaca ggtgctcggc gaggaataca gtaaccacaa agcgcaattg cgccgcgact
     4201 tcaaggtggt gctcgaccgc ctgctcggca cggcgttcaa tgactggcac cgcggccttt
     4261 atccgaccga agaaggcttc ggccgtaccg atgccttcgg tcgtattgcc aacagcgtgt
     4321 ttggtgatgc catcgatccg agcaactacc gcatcgccaa tgcaccagtg agttacccgc
     4381 aggtctggga tatctggaaa ttcgactggg tgcagtggaa tggctccgcc atgcagccca
     4441 tggcccgcaa cgtcggcgaa gccctggggg tcggcgctac cttgcgtttg ctcaataccg
     4501 aggggcaccc ggttgccgaa gccgagcgct acgcctccag cgtacgcttg cgtgacctgt
     4561 tcaccctgga agaaaccctc aagcaactgc aaccacccat ctggccggaa gaagtattcg
     4621 gcaaggtcga cctgccgctg gccagccgtg gtcgcgcgct gtttaccgag aactgcgctt
     4681 actgccacgc ccctgacccg aagaagcccg aggacagctt tgctccctcg cgtaacccgg
     4741 aatggcgtat gcgcgtggta ccgacctcga tcgtcggcac cgacccgacc actgcagaca
     4801 atatcgccga ccaccgcttc gatatcagca aactgggctg gaccaaggcc gagctgagca
     4861 agctggatgt gcgcctgttt ggcgccacgc tggacgatat cgacatgcgc agtatttcca
     4921 gcgccaaggc cctggcttat gtcaccgctt atgtggaaaa tcgcgcctat caggatgccg
     4981 gcatttccga gcgtgaacgc tggcgcatgg acggtttcgg cctgccgatt ggcgtgcagg
     5041 aaaaacgcgg ctacaaggcg cgcccgctgg atggcatctg ggccaccccg ccgtttctgc
     5101 ataacggttc agtgccgacc ttgttccagc tgctctcgcc cgtctcggaa cgccagaccc
     5161 agttctgggt gggcaacttc gagttcaatc ccaaacagat tggctaccag agcgagaaat
     5221 ttaccggcgg cttcctgttt gatacccggg tcaccggcaa cagcaatcgc ggccatgaat
     5281 tccgcgccgg ttgccgtgaa gacggcgtga tcggccgcgc cctgcaaccg gaagaacgct
     5341 gggcgctgat ctagtacctc aaggtcttgg gcaaccctgc gcaggaacag cagctccagc
     5401 ctgttgcagc caaggagtgg acgcccggcc cgtcttgcca gaactgaact gaacaccgcc
     5461 tgtgatcgca cactgagaat aattaaaagg aattgactcc atgtttaaac gattctggct
     5521 ctggctcggc cgcgtacttg gcaaattgct ggtgctcgta ctggtcatcg gtattggcgg
     5581 ctgggccatc ggggcggcct actacggctg gaaattctcc ggtccggttt ccactgaaga
     5641 acagatcccg gccgatgaag ccgcgctgac ccaaggcatc atcgaagatg ccattcgcgt
     5701 ggtcgaacag caccgcgaca atacccgcgt gctgcgtgat gcccacgcca aggcccatgg
     5761 ctgcatgaag gccgaagtta ccgtcagcgc tgatatcgat agcagcctgc aacgcggcgt
     5821 gctcagtgaa ccgggaaaaa cctggcaggc ctggatgcgc ctgtccaacg gcaatgccta
     5881 cccgcagttc gaccgggccc gcgatgcgcg cggtatggcg atcaagctgc tggatgtgcc
     5941 aggtgaaaaa ctcacgcaaa gcccgcagca cgccagcgag caggatttcg tgatgttcaa
     6001 tcacccagcc ttttttgtgc gcgacgtggc cgaatacaaa agcaatttcg ccgcccaggc
     6061 cgacggtaaa aaggccatgg ccttcttccc cagctggaac cccagcacct gggaaattcg
     6121 ccacctgatc atcgccctga agaccctctc gccggcgccg gaaacaccgg tggcgacgac
     6181 ctacaactcc atcgccccat tcaaactggg cgagcacaac atcaaatacc gagtcattcc
     6241 tcagccagaa gcctgccccg agtatcagtt gcctgagcag aaccaggacc tgccgaactt
     6301 tctgcgcaat gcgctgtacc agcagctgtc gttggaccgc gtaccggcct gctttgccct
     6361 gcaagtgcag cggcagaacg ccgagtacta catgcctatc gaagacccca gcgttgagtg
     6421 gagcgaagcc atctcaccgt ttgaaaccgt cgccaccatc aaggtgccgg cacaggattt
     6481 tgatagccgc gagcagaacc tattctgcga caacctgtcc ttcaacccct ggcacgcgct
     6541 gcccgagcat cgtcctattg gcggcataaa ccgcctgcgc aaggcagtct acgaagcggt
     6601 cagcatctat cggttggagc gcaatcagcc ataaaactgt atccaatcct ctgtgctact
     6661 cggttaacag ccattgagct ggtagtattc gcgccttgct aaacgcgctg ttagccgcgt
     6721 accacctgag agaaagaccg tggaattgtt caaagagttt atgttcgagg ccgcgcaccg
     6781 cctacctcac gtccccgaag gccacaaatg tgggcgcctg cacggccatt ccttcaaggt
     6841 ggcggtgtat atcgaaggtg aagttgaccc ttataccggc tggattcgcg acttttccga
     6901 gatcaaggcg atcttcaagc cgctctacga gcagctcgac cataactatc tgaacgatat
     6961 tcctggcctg gaaaacccca ccagtgaggt gttggccaag tggatctggc agcaactcaa
     7021 gccgctgctg cctgagctgt cacgtatccg catccacgaa acctgcacca gtggttgcga
     7081 ataccgcggc gactgatcgc attgcttgcc cggcactaca cagccaacgc cgggttagca
     7141 gcttcttacc ccccccagct caacgccaaa cgccctcaag cgctgcttga actctgcttc
     7201 gtcaatgtcc tgaacaccaa gctcacgtgc tttggtcaat ttcgaacctg cccctggacc
     7261 ggcgacgaca acactagttt tggcagacac tgagccagcg accttcgccc ccagcttttc
     7321 caattcggct ttggccttgt cgcggctgaa tgactccagg ctgccggtca gcacccaggt
     7381 ctgcccagcc aacggcaagc cttcgatcac tttcttttca ctcagccagt gcatgccaaa
     7441 ggcttttagc tgagcctcga tggcgcgtgc gcgctcaaca ttggcagcaa cttcgaaaaa
     7501 ttcacgcacg gcctttgcgg gcttttcatt caaggcttgt cgaagatcaa taccatcagc
     7561 agctattatt ttttccagcg agttctgttt caccgccaac ttttccgcgc ctgtagggcc
     7621 cacagaggga atattgagct tgtcaataaa tcctgccagt gatgcacaag cagcaaattc
     7681 tggacttatg ccgtgatcca tcaacggcgc aacactacct ctcataagct gtgcaattac
     7741 ctttcggtta tgagcatccg agaaaaaatt atgaatctca tgagccacct ctaaacctac
     7801 gtcaggcaaa taaatcaaaa cctccgaata ggctttccga attcgccgga gcgaacccag
     7861 cgaacgggcc agtaccttgg cggtttcctc gcccacatcg ggtatgccga gggcgtaaat
     7921 aaaccgcgcc agggtcggtt gcttactgtc ggcgatggcc tgcagcagct tgttgctgga
     7981 aacctcggca aagccttcca ggttgatgat ctgctcgaag gtcagcttgt aaagatcggc
     8041 cggtgaggca atcagctgct catcgaccaa ctgctcaatg gttttatcgc ccagaccttc
     8101 aatatccatg gcgcgacgtg agacaaagtg gatgatcgcc tgcttgagct gggcgccaca
     8161 ggccaaacgg ccaacgcagc ggtacaccga accctcgctg atggtttcct tgcccttgct
     8221 gcgcttaacc agctgggtgc gctcgacgtg ggagccgcac accgggcatt cagttggaat
     8281 actaatttga cgagcaagca gttcttcttc cgaccaatca gggcgctggt caagcaccac
     8341 ttgcatcacc tgcgggatga catcgccggc gcggcgaatg atcaccatat cgccaatcat
     8401 cagccccaag cgcgccacct catccatatt gtgcagcgtg gcattggcca ccattacacc
     8461 tgctactttg accggcttga ggcgcgccac cggcgtgacg gcgccggtac ggccgacctg
     8521 gaactcgacg ctgagtagct cggtcagctc ttcctgcgcc ggaaacttgt gggcaatggc
     8581 ccagtgtggc gttcgcgcac gaaagcccag ctgctgctgg tcttcgatgt tgttgacctt
     8641 aaacaccacg ccatcgatat cgtaggccag gctcatacgc cgctcaccga tatcgcggta
     8701 atagtccaga caggcctgca cgcctttggc cagcttcagc tcacgactga tgggaatgcc
     8761 ccaggctttg agctgctcaa gcatgccgac ctgggtggtc gccagttcgc cgctaacctg
     8821 gccgacgccg tagcagcaga attccagcgg acggctggcg gtaattctcg gatcaagctg
     8881 acgcaggcta ccggccgcag cgttgcgcgg attggcaaag gttttaccgc cactttcggc
     8941 ctggcgggca ttaagctcgt cgaaaccagc cttgggcatg tagacctcgc cacgcacctc
     9001 cagcacctgt ggccaaccct cgccctgcag cttcagcggc acattgcgga tggtgcgcac
     9061 gttgctgctg atgtcctcac cggtgctgcc gtcgccgcga gtggcaccgc gcaccagttg
     9121 gccgttctcg taacgcaagc tgacggccag gccgtcgagc ttgggctcgc agctatattc
     9181 gacatcggcg ccgccaccaa acagatcccc cgcgaggccc aaaccacttt gcacactgcg
     9241 gtcgaaggcc agcagatcgc cctcttcaaa tgcgttaccc aggctaagca tgggaatttc
     9301 atggcgcact tcgccaaatg cagcaatagc ctcgccaccg acgcgctggg tcggcgactc
     9361 ggctgttacc agttgcggat gctctgcctc cagcgcctgc agctcgcgga acaagcgatc
     9421 atattcggca tccggaatac tcggctcgtc gagtacgtaa tagcgatagt tgtgcccgtc
     9481 cagttcggca cgcagttgca caatgcgctg ggcagcagtc gaggcgtcgg tcattgcggt
     9541 gttctctcaa acgaaaagag cagccttggc tgctctttct acttcatggg ttaaatcagc
     9601 gcttattggt cagttgcttg cgctcgtatt cgacgatgcg ctggcgataa tgctcgatag
     9661 tttgtgcagt catcacgcta cgctggtcat ccttcaactc gccacccagt tcatgggcca
     9721 gcttgcgtgc ggcggccacc atcacatcaa aagcctgctt gggatgacgc gggccaggca
     9781 agccgaggaa gaaactcacg gcgcgggtgc tgaaaccttc aatatcgtcg agatcaaagg
     9841 tgccgggttt taaggcattg gccatggaga acagcacttc accattaccc gccatgcttt
     9901 cgtggcgatg gaagatgtcc atctcaccaa aacgcaggcc gctttccaaa atattctgca
     9961 gcagcgccgg gcctttaaag ccctcttcat cgcgagcgat gacattgatc accagcactt
    10021 cttcaaccgg tggcaattct ttgctgcttt ccttgcgttg actggtgggc ttaacaacat
    10081 cgtcatcaac cgggttaagc agcgtcggca cgggttcttc caaacctaga ttgaggtcac
    10141 cttgttgcgg ctcaccgctg cggcgacgat tcaactcacg cgcgctcagc gacggcaggt
    10201 cttcttcatc aaaagccggc tcatggctgc ggtcgataac ccgtggcggg ctgagcaaat
    10261 caggatcgct gtcatcatca ggcagattag aaaaactgcg gtcaagtttg aacttgagtt
    10321 tgcccttgcc accacgcata cgccgccaac catcaaacag gatgccggca atcacaataa
    10381 tgccgatgac tatcagccac tcgcgcagac cgaattccat gaaatacctt aacccctaat
    10441 aaaaatgtga tgaaaagggc cttcctggcc cattttgcta attcttttac gtgacgccaa
    10501 gcctatgttc tgtcaggcct ttccctgctt tataacgtca cgtctaataa caatgcgcat
    10561 taagctagca cgacgaaaga taactttaca ccgcctcgcg catcatgatt ccaccagcgc
    10621 cagcgcttct tccacatcca cggccaccaa tcgcgaacaa ccgggctcat gcatggtcac
    10681 gcccatcaac tgatcggcca tttccatggc gattttattg tgggtgatat agataaattg
    10741 caccgtttct gacatctctt ttaccagccg cgcataacgc ccgacgttgg cgtcatccag
    10801 cggcgcgtcc acctcatcga gcatgcaaaa cggtgccgga ttgagcttga agatggcaaa
    10861 aaccagggcc aaggccgtta gcgccttttc gccaccggag agcaggtgaa tggtgctgtt
    10921 cttcttgccc ggtgggcgcg ccatgatggt cacgccagta tccagcaggt cttcgccagt
    10981 cagttccaga taggcattgc cgccaccgaa caccttgggg aacagcgcct gtaggccgcc
    11041 attgatctgg tcgaaggttt ccttgaagcg gttgcgcgtc tctttatcga tcttgcgaat
    11101 cacgttttcc agagtatcca gcgcttccac cagatcggcg ttctgcgcat ccaagtaacg
    11161 cttacgctcg gattgctgct ggtattcgtc aatcgcagcc aggttgatcg gacccaggcg
    11221 ctggatacgt gcagccaggc gctccagctc ttcttcccaa gctttctcgc tggcctctgc
    11281 aggcaagctg gcgagcacac catgtaggtc gtaattgtct tccaacagct gatcctgcag
    11341 cgccttacgg cgtacggtca gggattgcca gtccatgcgt tgctgttcca actggccgcg
    11401 caacaactgc gcctgctgct cggcctgggt gcggcgtttc tccgcatcac gcagttcgcg
    11461 gtcggcatct tccagagcca gacgcgccaa tttgagttcg tcttccaccg ccatacgctt
    11521 gtcgagctgc tcttcgagct tcatgcgcag ttcttccagc ggcgcttcgc cctcctccag
    11581 attgaggttg agctgctcgc gcttctcatg cagacgctcg gcctgcaatt ccaagcgctc
    11641 cagagcctga cgggtggagt catgctgggc ttttagcgaa cccaatcgta cggccaactg
    11701 gtgggcgtga tccttgtgct ggcgcgcttc ctggcgcaca cgatcaaggc gttcacgcag
    11761 gctgtcacgg cgggccagca ggctttcacg ctgctcgttg tcggtcgcca tggcattcag
    11821 ggcgtcctgc agttgcagac gcgattcacc cagttgctca tgctcgattg cacgttgttc
    11881 gcccagctcc agcagctcgc tgtccaggcg ggtacggcgc agtatcagct gctcaacctt
    11941 ggcctgcccg gctgaaagct gagccttcaa ctcgccgagg cgacgcgctt ggtcctgcgc
    12001 ctgacggcgt tgctgctcgc gttgttcttc ctggacacgc tggttttcac gcaagtgcag
    12061 caaacgctca tcaagcaagg ccagtgccgc ttcgcgctta tcgcgctcca ggcttagccg
    12121 ttcgatttcc tgaccacggg ccagcacgcc gctttccgct tcactggcgc ggcgtacacg
    12181 caggaaatgc cggccgaccc agtagccatc acggctgatc aggctttcgc cttcggccaa
    12241 gctcaggcgc gcggccagcg cttcatccag gctctgcacc gccttgacct tggccagcca
    12301 gggtgccaaa tcgatgctgg cctcgacttt gtccagcagg ctgccggcaa cccgtgcgcc
    12361 gcccttgctc gggctggcca ggcgtagatc gccctgggtc aggccggcaa aatccagccc
    12421 ctggaaatca tccagcagca cggcctgcag atcggcaccc agcacggttt ccaccgccag
    12481 ttcccatccg gcttcaaccc gcaggccttc ggccaaacgt gggcgctcac tcagttgctg
    12541 ctcgcgcagc cattcaccgg cacctttgcc tgggtccagc gccgcttgct gcaaggcttc
    12601 caacgaggcc aaacggccat tcaggcgctg taattcgcct tgcgcctgct gctgagcctg
    12661 accgctgtgc tgcaggtcat tacgcagctg ctgtaggcgt tcggcctgct gctcttcttc
    12721 cagctgcaag tcttccaggc tgatttcgcc agcggcgatc tgctcgccca actcgataat
    12781 cgcggcatct tccgggtcgg ccgccagttg ctgcaactca tcggaaaggc ggcgctgacg
    12841 ctcggcgagg cgctccaggc tctgctccag ctgggcgatg cgtgattgct gcacctcggc
    12901 ctggcgccgc ggttcggcgc tgcgctggtt aaagctgtcc cactgctcct gccagccatg
    12961 catggctgtt tcggattctt ccaaaccagc ggcggactct tcggccgccg ctgcagtcag
    13021 ctcctgctca ggttcgagca tggccagctc ttcgcccaga gtcgccagca atgtgcggtc
    13081 gtggcccagg tgcgattcgg tttccaggcg cgcgcgctcg gcttcgttga ggtcgtcctg
    13141 caactggcgc agacgctgct gaccgtgctg gatgctctgc tcgatacggg caatgtcgcc
    13201 gcccaccgag tagaaacgac cctgcaccag gttaaaccgc tcgctcagct cgtgatgacc
    13261 atcgcgcaga cgttcgatgc tcgcatcggc gttgcgctgc tcggccacca gggcttcgaa
    13321 gctgacttcc tggttgctga tcaccgcctc gcggctaccc acctgctcgt tcagcgcctg
    13381 ccagcgcaag gcagtgagct gggctttgag ctgacgctct tcggccttgt attcctggta
    13441 tttctcggcc gactgggcct gacggtgcag gcggtcaagc tggcgctcca gctcttcacg
    13501 caggtcggtc aggcgcgcca ggttctcatg ggtgcggcgg atgcggttct cggtctcgcg
    13561 gcgacgttcc ttgtacttgg agatgccggc ggcttcttcg ataaagttac gcagatcttc
    13621 cggcttggct tcgatcagct tgctgatcat gccctgctcg atgatcgagt agctgcgcgg
    13681 ccccaggccg gtgccgagga agatgtcggt gatgtcgcgg cgacggcact tggtaccgtt
    13741 gaggaaatag gtgttctgac tgtcgcgagt gacccggcga cggatcgaga tttcgttgta
    13801 gccggcgtat tcgcccgtca gggtgccgtc ggagttatcg aaaatcagct cgatggaggc
    13861 ctgagtcacc ggcttgcggg tattggagcc attgaagatg acgtcggtca tcgactcgcc
    13921 acgcaggttc ttcgccgagc tctcgcccat cacccagcgt accgcgtcga taatgttcga
    13981 cttgccgcag ccattgggcc cgaccactgc cgccatattg ctgggaaagg ttaccgtggt
    14041 cgggtcaacg aatgacttga aaccggcaag cttgatgcac ttgagccgca tagacagcct
    14101 tccttaagcg tcggccagtg cagccagcac cagctggcaa ttgtgctcac cgtaacccag
    14161 caagacctcg cggacggcct ggtgatcacg gacgatgatg gcctgcagca atgcggcaaa
    14221 ggtattgagg aattggccca tttcaccctt acgccgctct agcgccaggt gataagtacg
    14281 gctgatggcc ggcagcaggt tctccacggt ttcctgcagg taaggattat tggagaacgg
    14341 gaaagcggca cgcatgatgt cgaaactgct ttcgacgaag ccgttgatat cttcacgctg
    14401 caggctgtcc tgcaaacgtt gttgaataac caggaacggc ccgagatcgg cctcaacctg
    14461 ccagctatct gctaccgctt tgcccagcag gatgtatagc tcaatgacca gggtgtacag
    14521 gctcttgacg ttatgcggcg tcagctcggc cacctgcgcg ccacggcgcg gcgggatcac
    14581 gatcaggtgc cgacgctcaa gaatcagcag cgcttcacgc acagaacccc ggctgacatt
    14641 cagcgcctgg gtgactttct gttcctgaat ccgctcacgc gccttcaact caccccgaat
    14701 gatgcgttcg gcaaggtgat gggcgatctg ctcagcgagg ctatccggtg ccttgaacgt
    14761 catgatgatc cttaggctct atctgaccta ttagagaggg ctgaaagcgg ctaaacccgc
    14821 gccagacttg gcctgagcgg tcgagcgcgg cgcgagtgta acacagggtg ttaccgggcc
    14881 aaggccacta gcgccatgct ttcaaaccaa aaggttggca gtcaggggat gggcatcaat
    14941 acagattgtc ctacaatcga gccaacacct actccaacaa taataagtac ggagccatca
    15001 tgttcgaacg cctaccctcc aactggatgc ttgtgattaa aagcatcggc tactggctct
    15061 ggttgatccc tgtattgggc atcccgctga gctattactg gtcattaaac agccaatatg
    15121 ccaatgcctg ggcctggctg gtgatctcgg tggtattcgg ccttatcccg ctgctggact
    15181 ttatcgtcgg ccgtgacccg gccaacccgg acgaagcagt acaggtgccg cagatggagc
    15241 aacagggcta ctaccgcctg ctcagcctgg ccactgtgcc cttcctgcta ggtatgctcg
    15301 gctgggctgg ctgggtattc gtcagctatg aagcctggag ttgggttggc cagttgggct
    15361 gggtgctgtc tgtaggcacg gtcacgggtg ccatcggtat taccgtggcc cacgagttga
    15421 ttcacaagga cccgaaactc gaacagaacg ccggcggcct gctgctagcg gcagtgtgct
    15481 acggcggctt caagattgag catgtacgtg gccaccatgt gcatgtatcg accccggaag
    15541 acgccagctc ctcgcgctac ggccagagcc tgtatgcctt cctgcctcat gcctacaagc
    15601 acaatttcct caatgccttc gcgcttgaag ccgagcgtct caaacgcaag aacctgcccg
    15661 ccctgcactg gcgcaacgag ctgctcgcct ggtacgccat cagcgccctg ttcctactcg
    15721 gcttcagcct cgccttcggc tggctcggcg cactgttctt tattggccag tcgatcgtcg
    15781 cctttaccct gctggaaatc gtcaactacg tggagcatta cggcctgcac cgccgcaagc
    15841 tggagaatgg ccgttatgag cgcaccaatc cgacgcactc atggaacagc aactttctgc
    15901 tgaccaacct gtttctcttc catctgcagc gccattccga ccaccacgct tacgctaaac
    15961 gccgctacca ggtgctccgg cattttgacg acagcccgca gttgcccaat ggttacgctg
    16021 gaatgatcgt cctggcgctg tttccgccgc tgtggcgcgc ggtgatggac ccacgggtac
    16081 gtgcctacta cgccggtgaa gagcatcaac tgacagattc gcagctgacc cactcctaag
    16141 cgcaatcgcc acaacaaaaa acgccaaggg cctgcaccct tggcgttttt ttgtttgctc
    16201 gactttatgt ttctgcgacg tcggcttggc ataaaagcgg ctggcgttta accgagctgc
    16261 ggggcttttc gcgctcataa ctgaatcgta tgacaatcgc ctgaccaaag agtcaaatat
    16321 ttgttgaccc taaagtcaga aaactctaga ttcgcctcat gccaagccgt gtggaaacgc
    16381 tgcgccgttg tgctcagtcg tttcagcaga gcctgatacg agaacaataa agtgcctgga
    16441 ggtcgtcctt gatccagttt ttactcaacc gggagctgcg tactgagcat gccctcgacc
    16501 caaacatcac cgtgctcaat tacctgcgtg agcatcttgg caaatccgga accaaagaag
    16561 gctgcgcctc tggcgactgc ggtgcctgca ccgtagtggt cggtgagctg attggggagc
    16621 gcgtgcgtta tcgcaccctc aactcctgcc tgacctttgt ctcctccttg cacggcaaac
    16681 agctgatcac ggttgaagac ctcaagcacc aaggtcaact gcacagcgtg cagcaggcca
    16741 tggtcgactg ccacggttcg cagtgcggct tctgcactcc gggtttcgtc atgtcgctgt
    16801 tcgccctgca gaagaacagc aacggcttcg acaaggccga caccatggaa gccctggccg
    16861 gcaacctctg ccgttgcacc ggttaccgcc cgattatcga cgctgccgag caagcttgct
    16921 gccagaagca gcctgatcag tttgacgcag ccgaagcgca aaccgttgcg caactgaaag
    16981 ccatcgcccc gcaggacacc gccgagctca acagcggcga caaacgctgc ctgttgccgc
    17041 taactgtcgc tgacctggcc gatatctaca cagccaaccc ggaagcgcgc ttgctcgctg
    17101 gcggcaccga cctggccctg gaagtcaccc agttccaccg cgagctgccg gtgatgatct
    17161 acgtcggcca tatcgaagca atgaagcgcg tcgacgtgac cgctgagagc atcgaaatcg
    17221 gtgccgctac cgcgctgtcc gattgctacg ccgccttggc tcaggattac ccggacttcg
    17281 gcgagctgct gcaccgcttc gcctctctgc aaattcgtaa ccagggcacc ctgggcggca
    17341 atatcggcaa cgcctcgccg attggtgacg ccccgccgct gctgatcgcc ctcggcgcgc
    17401 agatcgtgct gcgcaaaggc aacgccagcc gcaccctgcc gatcgaagac tacttcctcg
    17461 actacaaggt gactgcgcgc caggaagccg aatttatcga gaagatcatc gtgccgcgcc
    17521 atcaggccaa ccaggcgttc cgcgcctaca aggtgtccaa gcgcctcgac gatgacatct
    17581 ccgccgtctg cgccgccttc aatctggtgg tcgaagatgg cgtggtgcaa agcgcgcgta
    17641 tcgcctttgg cggcatggcg gctatcccga aacgcgcggc tgcctgtgaa gccgcgctgc
    17701 aaggctcagc ctggtatccg ggcgtgatcg aacgcgcttg cgccgcgctg gccgaggatt
    17761 tcaccccgct cagcgacttc cgcgccagca aggaataccg cctgctcatt gcacagaatc
    17821 tgctgcgtaa attcttcctc gagctgcaag ctcccgaagt cgaaacccgg gtgaccgcct
    17881 atgtctaacc acatccagca taaaacccag gccgaactgg ccgagttgtt ccgcgccgat
    17941 atcaccaccg gcgtcggcaa gagcgtcaaa catgaaagcg cggacaagca cgtctccggc
    18001 gaagccatct acgtcgacga ccgcctggaa tttcccaatc agctgcacgt ctatgcccgc
    18061 atgagcgacc gcgcccacgc gcgcatcgtc agcatcgaca ccgcgccttg ctacgccatt
    18121 cctggcgtgg ccatcgcgat caccagcaag gacgtaccag gccagctgga catcggccca
    18181 gtggtcgccg gtgacccgct gctggcggac ggtaaggtcg aatatgtggg tcaggtggtc
    18241 atcgccgtcg gcgccgacag cctggaaaca gcccgcaagg cggccatggc tgcaataatc
    18301 gaatacgaag accttgagcc ggtattggat gtggtcgatg cgctgcgcaa aaaacatttc
    18361 gtcctcgaca gccatcagca caagatcggc aactccgccg ccgaattggc caccgcgccg
    18421 cgccgcctgc aaggcagcct gcatatcggc ggccaggaac acttctacct ggaaacccag
    18481 atttcctcgg taatgccgac tgaagacggc ggcatgatcg tctacacctc gacgcagaac
    18541 gccaccgaag tgcagaagct ggttgccgaa gttctcggcg tgccgatgca caagatcgtg
    18601 atcgacatgc gccgcatggg cggcggcttc ggcggcaagg aaacccaggc cgctggcccg
    18661 gcgtgcctgt gcgcagtcat cgcccacctc accggccggc cgaccaagat gcgcctgccg
    18721 cgcgtcgaag acatgcagat gaccggcaag cgccatccgt tctacgtcga gtacgacgtc
    18781 ggcttcgatg acaacggtct gctgcatggc atcgaaatcg aactggccgg caactgcggc
    18841 tactcgccgg atctgtctgg ctcgatcgtc gaccgcgcga tgttccactc ggacaacgcc
    18901 tacttcctcg gcaacgccac catcaatggt caccgctgca agaccaacac cgcctcgaac
    18961 accgcctacc gcggcttcgg cggcccgcag ggcatggtcg ccatcgaaga gatcatggat
    19021 gcggtcgccc gcagcctggg caaagacccg ctggaagtgc gcaagaccaa ctactacggc
    19081 aagaccgagc gcaacgtcac ccactacccc cagaccgtcg agcacaacgt tatccacgag
    19141 atgaccgccg agctggaagc cagcagtgac tacgccaagc gccgcgaaga aatccgcgcg
    19201 ttcaacgcca agagccccgt gctgaagaaa ggcctggcgc tgaccccggt gaaattcggt
    19261 atcagcttta ccgccacctt cctcaaccag gccggcgcgc tgatccatat ctacaccgat
    19321 ggttcgattc acctgaacca cggcggcacc gaaatgggcc agggcctcaa caccaaggtc
    19381 gcgcagatcg tcgctgaagt gctgcaggtg gatatctcgc gtatccagat caccgccacc
    19441 aacaccgaca aggtgccaaa cacctcgccg actgccgcct ccagcggtgc cgaccttaac
    19501 ggcaaggccg cacagaacgc tgccgaaaca ctcaagcagc gcctggttga ttttctggtg
    19561 cgcgaataca aggtcacccc ggaagacgtc gaattccgca acagccaggt gcgcgtgcgt
    19621 gacctgttcc tgagcttcga ggaagtcatc caaaaggcct atttcggcca ggtatcgctc
    19681 tccagcaccg gcttctaccg cacgccgaag atcttctatg atcgcgacaa ggctgctggc
    19741 cgtcctttct actacttcgc ctacggcgca gcctgttcgg aagtgatcgt cgataccctg
    19801 accggtgagt acaagatgct gcgcaccgac atcctgcatg acgtcggtgc ctcgctgaac
    19861 ccggccatcg atatcggcca ggtcgaaggt gccttcgtcc agggcatggg ttggctaacc
    19921 atggaagagc tggtgtggaa cggcaagggc aagctgatga ccaacggccc ggccagctac
    19981 aagatcccag ccatcgccga tatgccgctg gacctgcgcg tcaaactggt ggaaaaccgc
    20041 aagaacccgg aagacaccgt gttccactcc aaggccgttg gtgagccgcc atttatgctc
    20101 ggcatcgccg tgtggtgtgc gatcaaagac gccgtggcca gcctggctga ctacaaggtg
    20161 cagccgaaca tcgacgcccc ggcaacgcct gagcgcgtgc tctggggggt ggagcagatg
    20221 aaaaaactcc agcaacccgc caaagccgca gcagcagaag tcgaaccggc atagaaccgt
    20281 aggagcgggc catgcccgct cctacaaaca gccaaaggtg tacaacaatg acaaaacaca
    20341 ccgaaacgaa accgcaaaca ccgcgaccaa ttccggtacc gatactggac ccgtcaccga
    20401 gcaactggcg cttaccacgc cccggacaga ccacgccttc ctggaggctg agcaaatgag
    20461 ttggatcagc gccctcgccg acctgcagca acgcggtgaa ccctgcgtgc tggtgaccat
    20521 catcgaagag cgcggctcaa ccccgcgcaa tgctggctcg aaaatggtgg tcaccgctga
    20581 ccgcatcttc gagaccatcg gtggcggtca tctggaattc aaggcgatgg agctggcccg
    20641 cgagatgctg caaagccgca gccaggacac ccgcctggag cgtttcagcc tgggcgccag
    20701 cctcggccag tgctgcggcg gcgccaccgt gctgctgttc gaaccgatgg gccaaccgca
    20761 ggcgcagatc gccgtattcg gtgccggcca cgtcggccgc gccctggtgc cgctgctcgc
    20821 cagcctgcca tgcaaggtgc gctggatcga ttcgcgggaa aacgaattcc ccgggcagat
    20881 ccccgctggc gtggacaagg tggtcaatga agaagtgatc gacgaaatcg aacgcatgcc
    20941 cgccggcagc tactttatcg tcatgaccca caaccaccag ctcgacctgg agctaactgc
    21001 agaaatcctc aagcgtaacg attttgccta ctacggtttg atcggctcgc taaccaagcg
    21061 cgcaaaattc gaacaccgcc tgcgtgatcg cggctttgac cctgcattgg tagcgcgcat
    21121 gcgctgcccc atgggcctgg ccgaggttaa aggcaagttg ccggtagaaa ttgccgtatc
    21181 catcgccggc gaagtgatcg ccacctataa cgccgccttt ggccaggacg taaaaaaagg
    21241 cgagacatcc gtcgccaagc tgctgcccgc gtcccgccgc gccactccgt agggtgcgcc
    21301 gcgcgcacca atatcccatt gaatccggtg cgcacggcgc accctacgca tgatcgagat
    21361 gtaaacgatg accaacactc tcaaagccta ccgcgccgcc atcctgcaca acatcgccga
    21421 ccccgccgtg gtcggcgtcg agcagtccta cgaatatttc gaggatggcg tgctggtcat
    21481 cgaaaatggt caggtcgccc gcgtcggcca tgccgccgag ctgttgccga cgctcaaggg
    21541 cgtcgaaatc accgagtacc gcgacgcact gatcaccccc ggttttatcg acacccatat
    21601 ccactacccg cagactggga tgatcgcctc ctatggcgag cagctgctgg attggctgaa
    21661 cacctacacc ttccccaccg agcagcagtt cgccgacaaa gcccatgcag cggacgtcgc
    21721 cggtatcttc ctgaaagagt tgctgcgcaa cggcaccacc accgcgctgg tgtttggcac
    21781 cgtgcacaag caatcggtgg acgccttctt cgaagccgcc gaagcgctca acctgcgcat
    21841 gatcgccggc aaggtgctga tggaccgcaa cgccccggac tacctgaccg acactccgga
    21901 atccggctac gccgacagca aggaactgat cgaacgctgg cacggcaagg gccgcctgca
    21961 ctacgcggtg accccacgtt ttgcccccac cagcaccccg gaacaactga ccctggctgg
    22021 gaaactgttg ggcgaatacc cggacctgta tatgcacacc cacctgtccg agaaccgcaa
    22081 ggaaatcgaa tgggtcaagg cgctgttccc cgagcgcaag ggctacctgg atgtgtacga
    22141 ccactacaag ctgatcggcg cccgctcggt attcgcccac ggcgtgcacc tatgcgacga
    22201 cgaatgcaaa cgcctggccg aaaccggttc ggccgtggct ttctgcccaa cctccaacct
    22261 gttcctcggc agcggcctgt tcgacctgaa caagctggaa cagcacggcg tgcgcgtcgg
    22321 cctgggcacc gacgtgggtg ccggcaccag ctttagccaa ctgcagtcgc tgaacgaggc
    22381 ctacaaggtc atgcaactgc agggcaagaa actcgatccg ttcaagtcgc tgtacctggc
    22441 caccctcggc ggcgcccatg cgctgtatct ggacgacaaa ctgggcaact tcgaaagcgg
    22501 caaagacgcc gacttcgtgg tgctcgacta caacgccacg ccgctgatca gctaccgcat
    22561 gcaacaggcc aagaccctgg aagaaagact gttcgccctg accatgctcg gcgacgaccg
    22621 cgccgtgaag gaaaccttcg ccgccggcca gtcggtgcat cgccgcgact aagatcgcca
    22681 agactaagcc aggcaggtta aatccctgcc aagaaaaagc ccgctcattg agcgggcttt
    22741 ttcttgatgc cggttgcgcc gatcagcggc ttgcggcttt cttcttgccg cttggcaaca
    22801 ggtgcgagaa caccgcgtgc aggtcatcgg aggcggtttc gccatccagg ttgagcttgg
    22861 cgtcaacgct gtccatatgg cgcatcatca ggtccaccgc cacgtctacg ttaccggcct
    22921 cgatggcatc aatcacctgg tcatgttcgg catccgagca atgggtgcgg ctgttgcttt
    22981 cgtagagggc gatgatcagc gaggtttgcg aaatcaggct gcgcagaaaa cccaataggg
    23041 tcaggttgcc ggcggcttcc gcgagcttca ggtggaactc acccgacagg cgaatgccca
    23101 taccgcgatc accacgggcg aagcagtcac gctcatcaat caccatctgg cgcaacgcgg
    23161 caatctgttc cggcttggcg ttcttgcagg ccagttcggt aatcgcccgc tcgatcatcc
    23221 ggcgtacata gaagatctgc cgcgcttctt ccgcaccagg gctggccact accgcgccac
    23281 ggttaggccg cagcagcacc acaccttcgt gggccaggcg cgataaagcg cgacggataa
    23341 tagtgcggct gacgccgaag atttcaccga gcgcctcttc actgagcttg gtaccaggag
    23401 ccagacgctg ctcaagaatg gcatcgaaga tatgggcgta aacgacatcg tcctgagtcc
    23461 cgctgcgagt gctcttgcct tcgcgcgtct gcttcttaag gggctgcaat tgttcgttca
    23521 ttgtggctcg cgtgatcaaa gttcggcggt tgctgatgca actctactgc gtgcgaccca
    23581 ccctctggca agcagcaaat cagtgaaaag tcacaaataa aaagcccttg cattgtacac
    23641 aatgtaacca gcacgtacac cgctaccaaa gcataaccat cttgcaaagg tgcatttgca
    23701 cacgctttaa gttcgcactt cctctaccga agaacgcgca gccggcagat tcatttctta
    23761 tatgcagcat ttttcttgtt cacgaatctg cttagcagcc gccccaagtg agtcacgaaa
    23821 gcaaccccgc tattcccgcg attattaccg aagacccgct gatcataggg gctgccagcg
    23881 cctcgtcgga atacctaacc ggatgctccg gtacacatca ggaaagtgac tggtattgca
    23941 taggtgaaaa agccgagtac atagcgaatt ttaaccagaa acattatttg ctttttttgt
    24001 atacaacatc ataatcggcc ctagcgttat ttcttgacct aaaggtcagc taagcacgcc
    24061 agcaaggcaa aaaacaccac ctacctcaag agtcgagcag tgcgcagcac aggctctggg
    24121 tggcacacaa caagagatga ggagtacccc tgtggaaagc gctaaacaag aacaacaggc
    24181 gac
//
LOCUS       DASRUM010000023        86462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1016410, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000023 DASRUM010000000
VERSION     DASRUM010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 86462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86462
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016410"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..2479
                     /locus_tag="VFF22_01600"
     CDS             110..2479
                     /locus_tag="VFF22_01600"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HZX15548.1"
                     /translation="MDLQQGFLLTRHWRDTPAGTQVEFWLATDNGPRHIRLPYQPSVA
                     FIPAAQREQAEALLQNERGVELRPLGLKDFHQRPVLGLYCQQHRQLMELDKLLRRAGV
                     EVYETDVRPPERYLMERFITAPVLFAGTPDPSGTGLLLDAQIKPCTDYRPTLKLASLD
                     IETNAFGELYSIAIEGCGQRNVYMLGRTPEQPPAVDFQLEYCASRGQLLERLNGWMAQ
                     HDPDAIIGWNLVQFDLRVLHEHARNLAIPLRLGRDGSEMQWREHGSRNGHFFADAAGR
                     LIIDGIEALRSATWSFPSFSLENVAQTLLGEGKDISTPYQRMDEIDRMFREDKPALAR
                     YNLRDCELVTRIFGKTAILDFLLERATVTGLPADRNGGSVAAFEHLYMPLMHRRGFVA
                     PNLGDNPPQASPGGFVMESQPGLYESVLVLDYKSLYPSIIRSFLIDPVGLIEGLRQPD
                     DAHSVPGFRGARFSRSEHCLPSIVERVWQGREAAKREHNAPLSQALKIIMNAFYGVLG
                     SSGCRFFDTRLASSITLRGHEIMARTRALIEAEGYAVIYGDTDSTFVWLKRPHAQEDA
                     AQIGRALVAKVNAWWREHLHNEYGLESALELQFETHYSRFLMPTIRGAEEGSKKRYAG
                     MVTHADGSHEMVYKGLETVRSDWSPLAQQFQRELYERIFLNQPYQAYVRDYIERTLNG
                     ELDELLIYRKRLRRKLTDYERNVPPHVRAARLADEYNAAHGRPLQYQRGGWISYVITT
                     AGPQPLEARSAAIDYDHYLTRQLQPVADAILPFVEDDFATLIGGQLGLF"
     gene            2519..3679
                     /locus_tag="VFF22_01605"
     CDS             2519..3679
                     /locus_tag="VFF22_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZX15549.1"
                     /translation="MLHTLAVANYRSINNLVLPLGRLNLVTGANGSGKSNLYRALRLL
                     AETAQGGVVNALAREGGLDSTFWAGPETLSRRMLNGEVPVQGGPRKEAKRLRLGFAGD
                     DFSYAISLGLPEPSLSAFALDPEIKRECIWAGASYRPASLLVDRAGPMVRIRDGRNWQ
                     VMAQHLPSFDSLFDQIGNDPACPEVFQLREHIRRWRFYDHFRSDAEAPARLPQLGTRT
                     PVLHHDGRDLAAALQTIREIGDPAALNAAISDAFPGARLRIDIQAGGRFAVELQQEGL
                     LRPLSASELSDGTLRYLLLVAALLTPRPPSLMVLNEPETSLHPDLLPALGRLIIAASQ
                     RTQVWVVSHASRLIATLEQHPECNAIVLEKQLGQTVIQGQGMLDEPAWKWPS"
     gene            3961..4494
                     /locus_tag="VFF22_01610"
     CDS             3961..4494
                     /locus_tag="VFF22_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZX15550.1"
                     /translation="MSTPAKLQTKQPQQLLFDSDGVIPNSPLPVLIYRQVELDGDLAA
                     AFERVFAVNQWPPQWRAGIFDYHHYHSNAHEALGVAAGNAEVMLGGEQGQAVHLQAGD
                     LVILPAGTGHCRIEQSADFLVVGAYPAGQEQYDMQRADASTHDASLERIARVATPQTD
                     PLYGGQGALAHAWGCKP"
     gene            4529..4852
                     /locus_tag="VFF22_01615"
     CDS             4529..4852
                     /locus_tag="VFF22_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008368314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZX15551.1"
                     /translation="MNAEYLANEPTRDALDALPGPTLIEFGTPWCGHCQAAQPLLAEV
                     FAGHPHVRHLKIEDGSGRRLGRSFKVKLWPTLIFFHDGQEVARLVRPTSVGQIEQAFL
                     TLTTP"
     gene            complement(4879..5784)
                     /locus_tag="VFF22_01620"
     CDS             complement(4879..5784)
                     /locus_tag="VFF22_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZX15552.1"
                     /translation="MNLSLYLLTVLIWGTTWIALKLQLGEVAIAASIAYRFALAAAVL
                     FAVLLLSGRLQKLDKRGQLICLAQGLCLFCINFMCFYTASQWIPSGLVAVIFSTATLW
                     NAINARLFFKQKIAVNVLAGGALGLGGLALLFWPELAGQEASRETLLGIGLALIGTLC
                     FSAGNMLSSLQQKAGLKPLTTNAWGMLYGALMLVAICLVSGTPFGFEWNTRYVGTLLY
                     LAIPGSVIGFTAYLTLVGRMGPERAAYCTVLFPVVALNISVFAEGYQWTAPALFGLVL
                     VMLGNVLVFRKPKALPATVALAAKG"
     gene            complement(6068..7282)
                     /gene="arsJ"
                     /locus_tag="VFF22_01625"
     CDS             complement(6068..7282)
                     /gene="arsJ"
                     /locus_tag="VFF22_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organoarsenical effux MFS transporter ArsJ"
                     /protein_id="HZX15553.1"
                     /translation="MFATLSPAVRQYLLVTGNYWAFTLTDGALRMLVVLHFHSLGYTP
                     LQIAALFLFYEIFGVLTNLVGGYLGARVGLNRTMNIGLGLQVAALLMLTVPAAWLTIP
                     WVMGAQALSGIAKDLNKMSAKSSIKLLVPDNQQGTLYKWVAILTGSKNALKGVGFFMG
                     GALLALLGFAGAVLAMAAVLALVWLASLLLLKKDLGKAKAKPKFRDILSKSRAINILS
                     AARLFLFGARDVWFVVALPVYLSSVFGWDFWMVGGFLAAWVIGYGMVQSFAPVITGKA
                     SGQLPDGRAAFAWAAMLAGLPAAIALGLNSDFSAQWVLLGGLMVFGALFAVNSSLHSY
                     LIVSYAKEDGVSLDVGFYYMSNALGRLIGTLLSGWVYQAYGLEACLWVSSGFVLLAAL
                     ISIGLPRHRAVA"
     gene            complement(7382..8377)
                     /locus_tag="VFF22_01630"
     CDS             complement(7382..8377)
                     /locus_tag="VFF22_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012316902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsJ-associated glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HZX15554.1"
                     /translation="MAIKVGINGFGRIGRLALRAAWGWPELEFVQINDPAGDAPTHAH
                     LLNFDSVHGRWQHEAGSDGDCVVIDGKRIQVTANKAIADTDWSGCDLVIEASGKMKTV
                     AVLQAYLDQGVKRVVVCAPVKEQGALNIVMGVNQQLFDPTQHRIVTAASCTTNCLAPV
                     VKVIHENLGIRHGSITTIHDLTNTQSILDTPHKDLRRARASGMSLIPTSTGSATAIAE
                     IFPELRGKLNGHAVRVPLANASLTDCVFEVERATTVEEVNALLKAAADGPLAGILGYE
                     ARPLVSIDYRTDPRSSIIDALSTMVVNGTQVKLYAWYDNEWGYANRAVELARMVG"
     gene            complement(8402..8734)
                     /locus_tag="VFF22_01635"
     CDS             complement(8402..8734)
                     /locus_tag="VFF22_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HZX15555.1"
                     /translation="MSMLNTLGLFALTALAEIIGCYLPYLWLKQGKSIWLLVPAAASL
                     ALFAWLLTLHPTAAGRVYAAYGGVYVAMAIIWLWLVDGIRPTHWDLLGAAVAMLGMAI
                     IMFAPRNI"
     gene            complement(8736..9230)
                     /locus_tag="VFF22_01640"
     CDS             complement(8736..9230)
                     /locus_tag="VFF22_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HZX15556.1"
                     /translation="MSHPFDVLSIPNRPGRLIFTPCPGSKDSSIDSALNTLKQAGADA
                     VLTLMPYAELAQNAASDLPALSAAQGLLWLHLPVADEQVPQADFDAAWPAAMAQIAEL
                     LAADKAIAIHCKGGSGRTGLIAARILIELGIARSEAISLVQALRSKAIQHPAHAGWIA
                     QFGN"
     gene            complement(9249..9959)
                     /gene="arsH"
                     /locus_tag="VFF22_01645"
     CDS             complement(9249..9959)
                     /gene="arsH"
                     /locus_tag="VFF22_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical resistance protein ArsH"
                     /protein_id="HZX15557.1"
                     /translation="MSPELPNIDPELLDPPSQEQLAKPAPLHKPRILLLYGSNRARSY
                     SRLLTEEAARLLEQFGAETRIFNPSGLPLPDDAPEDHPKVQELRELVLWSEGMVWCSP
                     ERHGAMTGVFKSQIDWIPLSMGAVRPTQGKTLALMQVSGGSQSFNALNQMRVLGRWMR
                     MFTIPNQSSVAKAFLEFDDAGRMKPSAYYDRVVDVMEELVKFTLLLRGQTDYLVDRYS
                     ERKESAEQLSKRVNQASI"
     gene            complement(9984..10757)
                     /locus_tag="VFF22_01650"
     CDS             complement(9984..10757)
                     /locus_tag="VFF22_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZX15558.1"
                     /translation="MSQKIRVLFLCVANAARSQMAEALLRHTDPEGYEVFSAGTLPGE
                     VDPRARQVLEQLGVSTEGLHSKPLESFDGQQFDYVITLCDKSAFECRTLPGAGEYIAW
                     DFEDPASSQQKNAYKHCLQQIHERIKMFVLVKSKAQAPLQMLAPVTLFKCLADDTRAR
                     MTQLIAQEGELCVCELTAALQESQPKISRHLAQLRSSGLLQDRRAGQWVYYRMHPQLP
                     AWVGQILAQVLQADDPQLAEDRLRLHSMRDRPDRASLCG"
     gene            10908..11984
                     /gene="arsB"
                     /locus_tag="VFF22_01655"
     CDS             10908..11984
                     /gene="arsB"
                     /locus_tag="VFF22_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HZX15559.1"
                     /translation="MNQALESSAPQTGSPLGLFERYLTLWVFLCIGGGTLLGLYAPEA
                     AQAVGALEIAQVNIPVGLLIWVMIIPMLMKIDFSAIGEVYQQRAGLGVTLSINWLIKP
                     FTMAFMAWLFLRHVFADWLPAEQIDSYVAGLILLGAAPCTAMVFVWSNLCKGNANFTL
                     TQVAINDLVMVFAFAPIVALLLGVSSIPVPWDTLLLSVVMYIVIPLAIAQFIRARLLR
                     HGDAALQAVLTRIGPFSILALLATLVLLFSFQGQTIVAQPLIIAMLAVPILLQTLFIA
                     ALGYWMCRQLKVRHDIAGPAAMIGASNFFELAVAVAIALYGFNSGAALATVVGVLIEV
                     PVMLWLVRMVNNSKGWYERTQVQG"
     gene            11987..12991
                     /locus_tag="VFF22_01660"
     CDS             11987..12991
                     /locus_tag="VFF22_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HZX15560.1"
                     /translation="MELLKWLNDQLLRMDWLAWLVRVLLEDGFDLDMTSRIGASLHFF
                     IYDVIKIFILLAVLIFSISWVQSYFPPERTRRILGGMSGFKAKLTGALLGTITPFCSC
                     SSIPLFIGFTSSGLPLGVTFAFLISSPLVDLASVILLASIFNWSIAIAYVLIGLVLAV
                     LGGTLIGRAKMERYVEAFVMHNPVLDIPQAELTRRDRAQFAWAQVSDIFRRVWLYVLI
                     GVGIGAAIHNWIPADWIEALLGQNNWWSVPLATLLGMPMYADIFGTLPIAEALVAKGI
                     GLGTALAFMMAVTALSLPSLIMLKKVVKAPLLALFFGIVTVGIVLIGYLFNAFAYLFI
                     "
     gene            13007..13240
                     /locus_tag="VFF22_01665"
     CDS             13007..13240
                     /locus_tag="VFF22_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZX15561.1"
                     /translation="MIIKILGSGCKKCITLTENTQAALANLGREAQVIKVTDFAKIAA
                     HGVMSTPALAIDDKVVSVGKVLTVGEVEKLLSA"
     gene            13342..14220
                     /locus_tag="VFF22_01670"
     CDS             13342..14220
                     /locus_tag="VFF22_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018927146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZX15562.1"
                     /translation="MSQLEHLQVFQSMHSSPNTRLHRSAELGDGLAAAVWSNRHDARD
                     YEAPSHHTLSCYLAGGTGTFRRESPHSKGAPDKLCILPAGHQSEWVINGEIHLAHLYI
                     SQEQFALAAVNLLDREPRELELREGTFLDDPQQAQRFRQLSQLDWQEPAERLLSSSLA
                     HGLINHALLSQVGRREGLRLTGGLAPSQRRRVREFIQANLSEALSLGQLASLCALSEY
                     HFARMFQLSFGLPPHRYVLSQRLRHGQQLLQHSTLALAEVALACGFASASHFNNRFRQ
                     AFSTTPGQYRAALQRP"
     gene            14396..17032
                     /locus_tag="VFF22_01675"
     CDS             14396..17032
                     /locus_tag="VFF22_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZX15563.1"
                     /translation="MALDILHLRQEDWRAEFGVGDLQRGQGYAAQGRSRLLSLKDSTL
                     LASCRGSGAQTYQQRITLHHYGQGWGVTGHCSCPVGFNCKHVASALLTLQTQQEQGVD
                     LSPLIVSSPQAREECLENLQPSPVLSLGSLVRVHFDARKGRMQEQTQHRAALAFDYQG
                     HLASGKQGKDLLIKQSAQHSLRIVRDISAETQLRKRLEQTGLQVALRQSEALPESAGE
                     AYEQPNETAWLQFVREQLPLLREEGWQIRMQPDFQYNLTPVDDWYAEVEEDPEQNWFD
                     LELGIEVEGQRISLLPILLQAIRRAPWLLNGEALNKRDDDEVLLVSLPHNHKRVALPL
                     ARLKPLLATLGELFVREPGESSQRLRLSRLDAARLNHLQQGPAISWQGGGQLRDFAER
                     LQRLDSATLQAPAGLEAELRPYQLQGLAWMQGLAELEVGGLLADDMGLGKTLQTLGHI
                     LCEFNAGRLQQPALIVMPTSLIPNWQDEAARFTPQLKVLALHGPKRRALFKQIDEHQI
                     ILTTYALLPRDLKALSAYRYRLLILDEAQNIKNPRSKAAVAAGQLQAGQRLCLTGTPL
                     ENHLGELWSLFNFLMPGWLGDSKSFTRDYRTPIEKNANQQRLTHLTGRIKPFILRRTK
                     EQVASELPPKTEITHWVELSAAQRDRYETLRLAMDQKVRAEIARQGLARSQIVILEAL
                     LRLRQACCDLRLLGDDGDSSLTSADSGKLSGLLEMLEELFAEGRRVLLFSQFTSMLAL
                     IEAELKARNIPYAKLTGSTQDRRTPVQQFQAGELPIFLISLKAGGSGLNLTAADTVIH
                     FDPWWNPAAEAQASDRAYRIGQDKPVFVYKLIARGSVEEKIQQLQQAKANLARGVLEG
                     GSQTQLQLSEDDLQALFAPLSD"
     gene            complement(17037..17543)
                     /locus_tag="VFF22_01680"
     CDS             complement(17037..17543)
                     /locus_tag="VFF22_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HZX15564.1"
                     /translation="MGKPNWAELMRERMHGLAESLGNLLMEAFHYLALFAIGAITAWA
                     AVMAFLGMLDKGHITVDDILLLFIYLELAAMVGIYFKTNHMPIRFMIYVAITALTRLM
                     ISDISHNHKPDMGVVMVSGAILLLALAIFVVRYASSRFPAVQAGGARKKTNAADEAAE
                     RDDAQSDD"
     gene            17812..18072
                     /locus_tag="VFF22_01685"
     CDS             17812..18072
                     /locus_tag="VFF22_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003188807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebG family protein"
                     /protein_id="HZX15565.1"
                     /translation="MAVEVVYRSSRDLERLFMDKAEADRHDKMLELAELLTEVLQKAV
                     PSLNEAQGEELGIYMAKNRDIFAKAFKNQPDALSELSEPAAE"
     gene            complement(18138..19157)
                     /locus_tag="VFF22_01690"
     CDS             complement(18138..19157)
                     /locus_tag="VFF22_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HZX15566.1"
                     /translation="MFAMMETARLESLHFAVDPQTGLQAMIAIHNSRLGPALGGCRYL
                     PYADTQSAIEDVLRLAKGMSYKAALAGLQQGGGKAVIIRPAHVDNRGALFEAFGRFIE
                     TLNGHYITAMDSGTSSVDMDCIAQHTQHVTSTTAEGDPSPHTAMGVFTGIRATALARL
                     GSDNLEGLRVAIQGLGNVGYALAEQLHAAGVELLVSDLDQGRVQLAVEQLGARPVASE
                     ALLATPCDILAPCGLGGVLNHTTVGQLRCSAVAGAANNQLASADISDSLQARGILYAP
                     DYVINAGGLIYVALKHQGEPLPAITAHLTQISRRLTEIFGHAQADKRSPARVADAIAE
                     RLLYG"
     gene            19310..20113
                     /locus_tag="VFF22_01695"
     CDS             19310..20113
                     /locus_tag="VFF22_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZX15567.1"
                     /translation="MTPRAACLACLEQNPPALFEAALWIAAEHQPTLQPAEVLQRLNG
                     LQQQVSQGLPNLPLQELAQPLLRRLSELDFHEDDERPLRPRAAMLDQLLLRRRGQPLS
                     LALLALELARRLGIPLQGVNFPGYFLLRVPGADHLLDPCSGRRLYTRECRELLQRHLG
                     PTAELSASQLQPCDARSMLQRLSRNLRQLHQLDDDPLAALRDAERVLLLGTPTLHDHL
                     ARAEIYQLLECPQGERFDLQCALLLSDDPAQHLQLSQRLRLLSASPALH"
     gene            complement(20311..22851)
                     /locus_tag="VFF22_01700"
     CDS             complement(20311..22851)
                     /locus_tag="VFF22_01700"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZX15568.1"
                     /translation="MNALAPFSLSAAASSCGSVQRPPPGHIARLSLQQRLLAHDCRLR
                     LLVGPAGFGKTVLLADCARECPANYRLLWLTCAGEGLHAEQLAQQLGKLLDYPADLSI
                     GELLATLTQESRPLWIMLNDYPAQPNHALDDFLNQLLMVTPPGICWWLGSRRRPACNL
                     SRLLLEGELLELGASELAFSEAEVFAWLQQLDPTRSAWAHDLHELTQGWPAALRLLLL
                     AAQAEPTALSAPQDGEHDQLLAEYVEREVLQGLPDELLQMLQQLAQLPRFNPALCDHL
                     FGVGEGAQWLRALIERGVFIQELDAAKGWYVLFPPLARLLQRQAANLPCSALHVHASQ
                     WFASHGDIRTAVEHALLAGQPEVAGSFLERFTEEQVLQGQDLALILRWRCELPESLLC
                     STPRLILLNAWVLLLVGRLDEAQTCINQLARFQPRSDGARTRELFAHWQAIQGIIAYG
                     RVCAVDARSHLHDALQGLPQSAWAQSLLCRSALTLVAIGEGQLEVAQKLSFEALKQAR
                     LHGNPVFEALLELDHALLLETRGEFARAEALLQRVLTATAPAALRNTPVLARLELRLG
                     RLRLRQGRADEAEAALQRGLEHALACGDPGAFHGYLGLAELAMGQGDMATAFAHLGQA
                     ERQMQRQHVTETLYRGTLLLASSHLWIAQGHYARARVAATRVLDYEQRVKAVLPTPNF
                     PELIPRFQALLLQLDRVQGEDVREPMRQLLEQVSAQGRQALLSDLWLSYAEACAASDD
                     KVGAEQARQTGQALQRRLGYLRPWFRVASQIDNAASAAPATNANALSSRELAVLGLIA
                     QGLSNQEVAEQLFISLHTVKTHARRINGKLGVARRTQAVAKAKALGFF"
     gene            complement(23010..24374)
                     /locus_tag="VFF22_01705"
     CDS             complement(23010..24374)
                     /locus_tag="VFF22_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011332510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HZX15569.1"
                     /translation="MNTRILQTGALVLTLLASSVMAAVSEQEAAQLGSALTPLGAEMA
                     GNADGSIPAWTGGLATDAGAVDDKGFLADPFANEQPLLTITAANAAQYQDKLSPGQLA
                     MLQRYSSYKIPVYPTHRTAAVPDAIYAAAKQSALKTTAIDGGNGLQNFADSRYYAFPI
                     PKSGVEVIWNHITRYRGGNVQRLIAQATPQTNGSYSIVKFADEVAVPSDMPDIDPAKA
                     ANVLLYFKQRVTAPSRLAGNVLLVHETIDQVKEPRLAWIYNAGQRRVRRAPQVAYDGP
                     GTAADGMRTADNFDLFSGAPDRYDWKLVGKKEMYIPYNSYKLDSPSLKYDDVLQAGHI
                     NQDLTRYELHRVWEVEATLKSDERHIYAKRHMYFDEDTWQLALVDHYDGRGQLWRVAE
                     GHAQNYYNHKTPGYTLEALYDLVAGRYLALGMKNEEKMAYNFGFKASAADFTPAALRN
                     SGVR"
     gene            complement(24408..26288)
                     /locus_tag="VFF22_01710"
     CDS             complement(24408..26288)
                     /locus_tag="VFF22_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HZX15570.1"
                     /translation="MTTRTRHAYFQPCALAVAVALGGIAPAQAVTFNIGEIEGEFDSS
                     LSVGASWSTQGADPDLIGTVNGGKAQASTGDDNRLNFKKGETFSKIFKGIHDLELKYG
                     DTGVFVRGKYWYDFELKDESRLFKDIDDHNRKEAAQSSGAQILDAFLYHNYNIADLPG
                     SVRVGKQVVSWGESTFIGNSINSINPVDAAAFRRPGAEIKEGLIPVNMFYVSQTLTDN
                     LSMEAFYQLEWDQTIADNCGTFFSSSDVVADGCDNNLTVPGSTTPEAVAGIRALQPFA
                     AATGVGFDIENEGVVVPRGGDRDARDSGQFGLALRWLGDNTEYGAYFMNYHSRTPFFN
                     TQTADAATFGQFAGAPAAADPSTTLGALCSAFGGFPACFGNATFNASVPSLAQVTALG
                     NGQYFLSYPEDIRLYGMSFSTVLPTGTSWAGEASYRPNAPVQINSTALNTALVNPLTG
                     GTAAPTIETTPGAENAGYRRKEITQVQTTFTHFFDQVLGASRLTVVGEIGAAHVGGLE
                     RTADLHYGRDSIYGAYSDANDDGFVTSTSWGYRMRGILEYSNAFAGVNLRPSVSWSHD
                     VDGYGPNGLFNEGSKAVSLGLDADYKNTYNASLSYTDFFGGDYNTSIDRDFVALSFGV
                     NF"
     gene            26865..27248
                     /gene="gcvH"
                     /locus_tag="VFF22_01715"
     CDS             26865..27248
                     /gene="gcvH"
                     /locus_tag="VFF22_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HZX15571.1"
                     /translation="MSELRFTVEHEWLRQDADGLVTVGITAYAQEALGDVVFVQLPEA
                     QSYAEGDEVAVLESVKAASNIAMPLDGEVVEVNAELESSPELVNEDPLGKGWFFRFRP
                     ANASAVADLLDQAAYERLLNANADA"
     gene            27258..30110
                     /gene="gcvP"
                     /locus_tag="VFF22_01720"
     CDS             27258..30110
                     /gene="gcvP"
                     /locus_tag="VFF22_01720"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HZX15572.1"
                     /translation="MTKLTTQNEFVARHIGPRDADTAAMLELLGYASIDALTYNVIPE
                     SIKGTSVLGAQPGLSEADALAKIKTIAGKNQQLKTFIGQGYYGTHTPSPILRNLLENP
                     AWYTAYTPYQPEISQGRLESLLNFQTLISDLTGMQIANASLLDEATAAAEAMTFCKRL
                     SKNKASNAFFASQHCHPQTLDVLRTRAEPLGIEVVIGDEAAITDASAYFGALLQYPAS
                     NGDIFDYRELVERFHAAGALVAVAADLLALTLLTAPGEFGADVALGSAQRFGVPLGFG
                     GPHAAYFATRDAFKRDMPGRLVGMSIDRFGKPALRLAMQTREQHIRREKATSNICTAQ
                     VLLANIASMYAVYHGPKGLTQIAQRVHQFTAILAKGLRALGYSVEQEFFFDTLTLATG
                     GKTAALHAAARKAGINLREVDSERLGLSLDETSDQAAVEAQLAVFAEGKAVPAFAELA
                     AGVIAQLPKGLLRESEILTHPVFNRYHSETELMRYLRKLADKDLALDRSMIPLGSCTM
                     KLNAASEMIPVTWAEFGSLHPFAPAEQSQGYTQLTTELEAMLCAATGYDGISLQPNAG
                     SQGEYAGLLAIRAYHLSRGDDQRDICLIPQSAHGTNPATASMAGMRVVVTACDSSGNV
                     DIADLKAKAEEHKERLAAIMITYPSTHGVFEEGIREIAQIVHDNGGQVYIDGANMNAM
                     VGLCAPGKFGGDVSHLNLHKTFCIPHGGGGPGVGPIGVKEHLIPFLPGHGHMARKEGA
                     VSAAPFGSASILPITWMYISMMGGEGLKRASQMAILNANYIARRLEEHYPVLYSGSNG
                     LVAHECILDIRPIKDSSGISVDDVAKRLIDFGFHAPTMSFPVAGTLMIEPTESESKEE
                     LDRFCDAMIAIRNEIRAVEAGQLDANDNPLKNAPHTALELVGEWTHAYSREQAVYPVA
                     SLIEAKYWPPVGRVDNVFGDRNLICACPSIEAYQDA"
     gene            30257..31510
                     /gene="glyA"
                     /locus_tag="VFF22_01725"
     CDS             30257..31510
                     /gene="glyA"
                     /locus_tag="VFF22_01725"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZX15573.1"
                     /translation="MFSKHDQLQGYDEALLAAINAEEQRQEDHIELIASENYCSQRVM
                     QAQGSGLTNKYAEGYPGKRYYGGCEHVDKVEALAIERAKQLFGADYANVQPHSGSSAN
                     SAVYLALLNAGDTILGMSLAHGGHLTHGAKVSSSGKLYNAVQYGIDEQGLIDYAEVER
                     LAVEHKPKMIVAGFSAYSRTLDFARFRAIADKVGALLFVDMAHVAGLVAAGLYPNPLP
                     FADVVTTTTHKTLRGPRGGLILAKANEEIEKKLNSAVFPGSQGGPLMHVIAAKAVCFK
                     EALEPGFKTYQQQVINNAQAMAEVFVARGYDVVSGGTDNHLMLVSLIKQGLTGKAADA
                     ALGAAHITVNKNAVPNDPQSPFVTSGIRIGTPAVTTRGFKQGECRTLAGWICDILDDL
                     ENPAVIERVRGQVADLCASFPVYAN"
     gene            31634..33010
                     /locus_tag="VFF22_01730"
     CDS             31634..33010
                     /locus_tag="VFF22_01730"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HZX15574.1"
                     /translation="MSLSVFDLFKIGIGPSSSHTVGPMLAALRFAEGLRRDDLLATTD
                     AVKVELYGSLGATGKGHGSDKAVLLGLEGEQPDTVDTSSVDDRLATIRSSGELNLLGE
                     KAIRFVEKQHLAMIRKPLAFHPNGMIFRAFDAAGLQIRSREYYSVGGGFVVDEQAAGA
                     DRIVEDSTVLQYPFTTGKQLLAHCAEHKLSISQVMLANEAAWRPEAETRARLLHIWQV
                     MQDCVEAGCRNEGIMPGGLKVKRRAADLHRQLCKNPEAALRDALSVLDWVNLYALAVN
                     EENASGGRVVTAPTNGAAGIVPAVLHYYSRFIPSSNDDGVVRFLLTAAAIGILYKENA
                     SISGAEVGCQGEVGVACSMAAGALCEVLGGSVNQVENAAEIGMEHNLGLTCDPIGGLV
                     QVPCIERNAMGSVKAINAARMALRGDGQHFVSLDKVIRTMRQTGADMNNKYKETARGG
                     LAVNIIEC"
     gene            33079..34200
                     /gene="gcvT"
                     /locus_tag="VFF22_01735"
     CDS             33079..34200
                     /gene="gcvT"
                     /locus_tag="VFF22_01735"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZX15575.1"
                     /translation="MSTETLAKTPLHALHLELGARMVPFAGYDMPVQYPLGVMKEHLH
                     TRESAGLFDVSHMGQIILRGPQAAQALERLVPVDIIDLPVGMQRYAMFTDDQGGILDD
                     LMVANLGNDTLFLVVNAACKHQDLMHLQLQIASQCQIEACFDRALLALQGPKAVDVLA
                     RLAPEVAKMTFMQFAPVRLLGVDCYVSRSGYTGEDGFEISVPAAHAETLARSLLAEPE
                     VAAIGLGARDSLRLEAGLCLYGHDMSTSTTPIEASLLWAISKPRRADGARAGGFPGAE
                     RIFAQQQQGVASKRVGLLPQERVPVREGAEIVDADGTVIGTVCSGGFGPTLAAPVAMG
                     YLQSSHIALDTEVWAMVRGKRVAMKVAKTPFVPQRYYRG"
     gene            complement(34252..34617)
                     /locus_tag="VFF22_01740"
     CDS             complement(34252..34617)
                     /locus_tag="VFF22_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HZX15576.1"
                     /translation="MQLAAPIPILRSFDEAKARVFYCEFLGFSEDWAHRFEAGLPLYL
                     QVSRAGCVLHLSEHHGDCCPGAALRISVDDLDALHAELTARAYGNARPGITSQPWGRD
                     MAVTDPFGNRLIFTDIRAA"
     gene            complement(34680..35036)
                     /locus_tag="VFF22_01745"
     CDS             complement(34680..35036)
                     /locus_tag="VFF22_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate isomerase"
                     /protein_id="HZX15577.1"
                     /translation="MPHCLVEGSQTLTRLITPAELLGVVEDAAAATQLFQPGEVKLRL
                     SLYEHFSVGGTQEDFVHLIFYILAGRTDEQKRLLSRQIVRALVERLPSVNAVSLDVRD
                     IDREVFSNKRNCLLPD"
     gene            35643..36905
                     /locus_tag="VFF22_01750"
     CDS             35643..36905
                     /locus_tag="VFF22_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015802 - basic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family porin"
                     /protein_id="HZX15578.1"
                     /translation="MTARNALSLALLTTAGSMALALPLSSQAAGFIDDSKVNIGLRNY
                     YFNRNFLNHTGPAVNGDDQGQAAEWTQSMIIDARSGFTQGTVGFGLDALVLGAIKLDS
                     GGGSTGSGLLPKHDDGDSADSYGRVAVAAKAMLSKTELKVGEWFAVLPILRADDGRAL
                     PQTFQGAQITSNEFDGLTLYGGQFWKNSQRNDASREEMSYSGVEGDDFNFGGGEYKFN
                     GNNTLVGAWHARLEDVYQQSYVQLTHSQPVGDWVLSTNLGYVDGKDEGAAKAGKLDNK
                     VYQGSFTAKTGNNAFTLAYQHLDGDTKFMRIDGASGGTLVNDGFTNSYDNPEERSWQV
                     RHDYNFAGLGIPGMTLMNRYVSGDNIKTAGRDDAEEWGRESELAYTVQSGALKNLNVK
                     WRNSTVRRDVGQDLHENRLIFNYPLSLL"
     gene            complement(37043..38173)
                     /locus_tag="VFF22_01755"
     CDS             complement(37043..38173)
                     /locus_tag="VFF22_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HZX15579.1"
                     /translation="MQISSNFDSGNIEVIDASNPQQVLLAIRPDLNSGHFQWFHFKVE
                     GLQPGQRYGFSLGNAGQSSYPNAWSGYHTVASYDQSDWFRVPSRFADGALSFDIEPQH
                     EQIWFAYFEPYSRERHSQLIEDAQRLAGAELFATGKSIEGRPIELLRIRRNPQAQRKI
                     WLIAQQHPGEHMAEWFMQGLLERLQLRDDAELNALLDKADLYLVPNMNPDGAFRGHLR
                     TNVAGRDLNRAWQSASEADSPEVFFVQQQMRAVGVDLFLDIHGDEEIPHVFVAGCEGN
                     PGYSPRLQRLENQFKQLLIDNGAEFQTTFGYTPDQFGQANTSLACNAVGMEFDCLAFT
                     IEMPFKDHDDNPNPHTGWNGERSMQLGKDVLSVVAAMVDDLR"
     gene            38403..38945
                     /locus_tag="VFF22_01760"
     CDS             38403..38945
                     /locus_tag="VFF22_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZX15580.1"
                     /translation="MNIAPLRHRPLLLLLCTGLLGGCASQPEQSQTEQPTEQADAPRE
                     NSVAEANLRSQANRGDLDSQFQLGSLYFVGKPNKDLKQAEYWWKLAADKGHAMAAVSL
                     AYLYTGRDNPDFNNPQQMLKYLNQSAAGGNPMAQHILGNLYLRGEQGVPLDANQARRL
                     FTSACQQNYSVSCEALQKLP"
     gene            complement(39090..40028)
                     /locus_tag="VFF22_01765"
     CDS             complement(39090..40028)
                     /locus_tag="VFF22_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HZX15581.1"
                     /translation="MNKPQVLQIGPLSERFNRTLAIRYEVTALWQQADPLAFLYEQGG
                     QFELLVSSARFGCSAEQLACLPNLRAICSFGVGYDPYPLELLRDRGIVISTTPDVLND
                     CVADLAMGLIIDTARQLAAADRFVRQGQWLAGNYPLTRKVSGKKLGIVGLGRIGQAIA
                     RRAEGFAMPVRYHKRRQDPSSPYGFEADLLALAHWADFLVLACPGGAATQHLIDAPVL
                     AALGPQGILVNIARGSVVEQSALVAALLDGSLGGAGLDVFADEPEVPPALFALPNVVL
                     LPHVGSGTQETRLQMEELVLANLQAFINKGELLTPL"
     gene            complement(40133..41689)
                     /gene="garD"
                     /locus_tag="VFF22_01770"
     CDS             complement(40133..41689)
                     /gene="garD"
                     /locus_tag="VFF22_01770"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HZX15582.1"
                     /translation="MQLIPHQDSPRYIRLHPADNVGVVVNEQGVAKGGRFADGLVAVE
                     GIPQSHKVALQDIAVGGDVVRYGEVIGYALEPIAAGRWVTEAQLRMPEPPALDNLPKA
                     TLAAAPVEPLTGYTFEGFRNPDGTVGTRNILGVTTTVQCVVGVLDHCVERVRKELLPK
                     YPYVDDVVALSHSYGCGVAINAPDAVVPIRTLYNISRNPNLGGQALVISLGCEKLQAS
                     QLMDGDQLTNGMDEEEWLFRLQDSSNGFAGMVEQIMQMIEARLQVLNERRRETVPASE
                     LSVGMQCGGSDAFSGITANPALGVAADLLVRAGATVMFSENTEVRDGIHLLTPRAATV
                     EVADALIREMDWYDRYLQRGMADRSANTTPGNKKGGLNNIVEKAMGSIAKSGSSPIAG
                     VVSPGERVRGKGLWFCATPASDFICGTLQLAAGMNLHIFTTGRGTPYGLAMVPVIKVA
                     TRTQLAERWPDLIDVDAGQITSGRMTLDEMGWHIFQLYLDVASGKQQTWAEKHRLHND
                     LTLFNPAPVT"
     gene            complement(41704..43059)
                     /locus_tag="VFF22_01775"
     CDS             complement(41704..43059)
                     /locus_tag="VFF22_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX15583.1"
                     /translation="MQAVKPGRVRYSILLMLFLVTTVTFADRSSLSIAGAALQADLGI
                     DAVMLGYIFSAFGWAYVIGQIPGGWLFDRFGAKRVYFITLLCWSLFTLLQGFVDMLPL
                     AWAIGSMFLLRLAVGFAGAPCFPGNARIIAAWFPTRERATATAMSTSAQYAATAVFAP
                     LMGWVVQDMGWQYVFFVLGGLGLLLCGVWLKVMHSPREHPHLGAAEFAHIEGGGGLID
                     LAPLPSAAGGSQWRTLKLLLTQRTLIGLYLGQYCNNAITYFFLTWFPVYLVQARGMTI
                     LAAGFAASLPAISGFVGGVLGGLVSDGLLRRGYSLTLARKLPIVVGMLLASCIVLSIY
                     VESDSAVIALMALAFFGKGFGSLGWTLVADTSPRQIVGLSGGLFNTFGNLAAITTPIV
                     VGYLVSHTGSFDAALIYVGANAVLAVISYLFIVGRIHRIELDEPPGPAADRAVAASNL
                     K"
     gene            complement(43147..44502)
                     /gene="gudD"
                     /locus_tag="VFF22_01780"
     CDS             complement(43147..44502)
                     /gene="gudD"
                     /locus_tag="VFF22_01780"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003282639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HZX15584.1"
                     /translation="MTTQNTAGAFSGAPIITELQVVPVAGHDSMLLNLSGAHGPFFTR
                     NILILKDNAGHIGVGEVPGGEAIRQTLEDARALLVGQSIGNYNGLLNQVRKTFADRDS
                     GGRGLQTFDLRITIHAVTALEAALLDLLGQHLNVPVAALLGEGQQRDAVEMLGYLFFI
                     ADKGKTDLGYRDERNADDAWFRTRNETALSPETIVRQAEAAHARYGFNDFKLKGGVLQ
                     GAEEVEAIRALAARFPEARVTLDPNGGWSLAEAVHLCRDLHGVLAYAEDPCGAENGYS
                     GREVMAEFRRATGLPTATNMIATDWRQMGHCIQLQSVDIPLADPHFWTMQGSVRVAQM
                     CNEWGLTWGSHSNNHFDISLAMFTHVAAAAPGKVTAIDTHWIWQDGQYLTHNPLQIEG
                     GLVQVPKTPGLGVELDWDALAKAHELYKGMGLGARNDAVAMQYLIPNWQFDNKRPCLV
                     R"
     gene            complement(44670..46112)
                     /locus_tag="VFF22_01785"
     CDS             complement(44670..46112)
                     /locus_tag="VFF22_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014597941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZX15585.1"
                     /translation="MANPSRYDNFIGGQWVAAASYSTNTSPSDLSDVIGEYAQADAAQ
                     VDQAIAAARAAFPAWSTSGIQARSDALDKVGSEILARREELGTLLAREEGKTLPEAIG
                     EVSRAGNIFKFFAGECLRQAGELLASVRTGVSVEVTREPLGVIGLITPWNFPIAIPAW
                     KVAPALAYGNCVVLKPADLVPGCAWAIAEIISRAGFPAGVFNLVMGSGRVVGEAIVQN
                     SQVDGVSFTGSVGVGRNIAANCVARGAKVQLEMGGKNPQVILDDADLKTAVELSVQSA
                     FYSTGQRCTASSRLIVTAGIYDQFVAAMAERMKSIKVGNALEKGVDIGPVVSQAQLEQ
                     DLRYIEIGKQEGARLVCGGERVSCEQDGYYLAPTLFADSTADMRISREEIFGPVANIV
                     KVADYDEALAMANDTEFGLSAGICTTSLKYANHFKRHSQAGMVMVNLPTAGVDYHVPF
                     GGRKGSSYGSREQGKYAQEFYTTVKTTYIG"
     gene            complement(46167..47078)
                     /gene="kdgD"
                     /locus_tag="VFF22_01790"
     CDS             complement(46167..47078)
                     /gene="kdgD"
                     /locus_tag="VFF22_01790"
                     /EC_number="4.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017709392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047448 - 5-dehydro-4-deoxyglucarate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxyglucarate dehydratase"
                     /protein_id="HZX15586.1"
                     /translation="MNPQELKSILSSGLLSFPVTDFDAAGDFRADTYARRLEWLAPYG
                     ASALFAAGGTGEFFSLTGKEYSAVIKTAVDTCAGSVPILAGVGGPTRQAIEMAQEAER
                     LGAKGLLLLPHYLTEASQEGVALHVEAVCKSVKIGVVVYNRSVCRLTAPLLEQVADRC
                     PNLIGYKDGIGDIELMVSIRRRLGDRLSYLGGLQTAEVYAAAYKALGVPVYSSAVFNF
                     IPKTAMNFYHAMARDDHATVGKIIDEFFLPYLAIRNRCAGYGVSIIKAGAKIVGHDAG
                     PVRTPLTDLLPEEYEMLAALIDTQGAQ"
     gene            47740..49140
                     /locus_tag="VFF22_01795"
     CDS             47740..49140
                     /locus_tag="VFF22_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZX15587.1"
                     /translation="MFEQAPTHTPHVVTMQVIPVAGRDSMLLNLCGAHAPYFTRNLVL
                     LTDSAGRTGCGEVPGGEGIRQALERCRDLVIGQPIGRYNRVLNSLRQAIAGRGNGPQT
                     TQHQVTSESEARVLQQPHEINLRLDNVITAVEAALLDLLGQHLNVPVAELLGSGQQRD
                     RVPMLAYLFYIGERQRTDLPYLTGKGSTDDWYHLRHQAALTPDAIARLAEAAHARYGF
                     NDFKLKGGVMRGEEEMDAIRAIKARFPDARVTLDPNGAWSLDEAIALCQGQGHVLAYA
                     EDPCGPENGYSGREIMAEFKRATGIPTATNMVATDWRQMGHSLRLEAVDIPLADPHFW
                     TMQGAVRLGQVCEEFGMTWGSHSNNHFDISLAMFTHAAAAVPGRITAIDTHWIWQEGE
                     ERLTREPLQIVGGQVEVPDKPGLGIEPDMQQIMAAHELYKKVANGARDDAMAMQYLVP
                     GWQYHPKQPSLGRQPA"
     gene            49183..50151
                     /locus_tag="VFF22_01800"
     CDS             49183..50151
                     /locus_tag="VFF22_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZX15588.1"
                     /translation="MKKTLCASLMSAMLTFTAGSAFAADAPNWPTRPVQVVVIANPGG
                     DTDFNARMMAKYFNQITGKTMVVTNVAGGGGTLAAEQVKGAAADGNTILFTHPGQLIV
                     NEVAGLTEDSYETFDVACFAGVDKSTVFVASKDAGVNTMQELIDKSKAAPSSITYGTE
                     MGSFSHAQGLMLEKLSGIKLKMVDGGTVSDRVVGMLGGRLDLGAITYGSVQDYVNGGQ
                     MVALGQPNDARNPLLGDVPTLKEQGLDLTMDKPYVVAFPKGTDPAIVAKMADLMKQIT
                     EIPEYAEDLKKFKQPVAYFGTEDAKAILAKTRDDFMQFKDELRQGK"
     gene            50216..50743
                     /locus_tag="VFF22_01805"
     CDS             50216..50743
                     /locus_tag="VFF22_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HZX15589.1"
                     /translation="MNTTANKKELIIGLAMTGCSLAYLVMAYQLPNHQGVDAATLPLL
                     LAGFLTLLGVLQVFGALASKPALGTERASDTQGELPASPAEAPPASVVEPKTVLLTLG
                     LILGYIALLGPVGFPLMTVVYLYLQFLVLTPVQHKANHLVYLLIAVVCSAVIFLLFRQ
                     AFDLMLPAGLLNNFI"
     gene            50753..52273
                     /locus_tag="VFF22_01810"
     CDS             50753..52273
                     /locus_tag="VFF22_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HZX15590.1"
                     /translation="MLELLQQGFGAVFSLNIMLLMAVGVAMGIVFGAVPGLSATMAVA
                     LCLPLTFTMGPQAGLSLLVALFIGATSGGLISAILLKIPGTPSSIATVFDGGPLMEQG
                     HGVKALGVGIVFSFLGTIFSIVALMFIAPQLAKVALRFGPHEYFAIAVFSLTLIATLS
                     AGSMLKGLFAGGLGIAVSTVGIAPVEAIRRFTFDFSELNGGFSMLAVMIGMFAVAEII
                     KLAETGRHAVQGKARSVSMKNIKGFGFSLKEFREQLPNAGRSGLIGLGIGILPGIGAG
                     TSNLVSYIIAKKRAKDGHTYGKGNIGGVVASETANNAGIGGAMLPLMTLGIPGDTVTA
                     IMLGGFLIHGIQPGPLLFISQGPLVYTIFAALIVATFMMLFMEFYGLRLFIKLLDVPK
                     HILLPIILVLCVVGAFGLSSRLFDVWSILLFGLLGYGFVKAGLPAAPFIIGFILGPMA
                     ETNLRRGLMLSDGNFADFFTTPIAGSFLGLALAFIIWQVVSSLRTKKSMVSEILRT"
     gene            52576..53295
                     /locus_tag="VFF22_01815"
     CDS             52576..53295
                     /locus_tag="VFF22_01815"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010562984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX15591.1"
                     /translation="MRNEELKALFDQQAAGYDKQWAGMAPIRDALHFLLNALFADLPE
                     NARILCVGVGTGTELAHLAERFPGWYFTAVDPSGAMLDICRQRAEKGGFLSRCSFHQG
                     YLDTLPAEPRHDGATCFLVSHFLLDPQARIALFHEISRRLRADGILANVDLASDVQSP
                     AYELLLRSWMTLMCSAGVPPDVLERSRAAYARDVAILPAQQVASLIKEAGFEAPVQFF
                     QAGLMHGWFCTLRPRPTCAVA"
     gene            53268..53442
                     /locus_tag="VFF22_01820"
                     /pseudo
     CDS             53268..53442
                     /locus_tag="VFF22_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241547.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
     gene            complement(53480..54751)
                     /locus_tag="VFF22_01825"
     CDS             complement(53480..54751)
                     /locus_tag="VFF22_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HZX15592.1"
                     /translation="MKGIKLSFGLSTKIFVGFISGMLFGLIAPEWGVAIKPIGDAFIR
                     AIQMLIIPIVFFSVSAGIANMGDVAQLKRVGGKTLVIYTVMTMLAGLVGLVVANLLQP
                     GVDFAGNGNSVAPVGDVPTIGQFLLGMIPSNIFDAMAKGSVIQIILIAVLVGVALVFL
                     GDRASGIKTLCTQASTLILKILDMVMALSPYGIFALMAYSMGNYGLDIFNQVGQFILA
                     DYIGLLVVLLLTSTVVVTYTKINPLYLLKKISKVWMVTLSTTSSAATLPMSMHVTSED
                     LKVPGWITSFMLPLGATINLMGAAVYLSVLVVFAAGFYGVDLSVSMMINVVMVGTLLS
                     MAAPGIPGGGIVMGTLMLQLMGLPFELVGVIAGIYRVIDMGHTTLNVTGDVFGALLVA
                     KSEGLLQCSDGGIPLAQASLEPDAPPVLVAR"
     gene            complement(54941..56119)
                     /gene="gltS"
                     /locus_tag="VFF22_01830"
     CDS             complement(54941..56119)
                     /gene="gltS"
                     /locus_tag="VFF22_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HZX15593.1"
                     /translation="MNISLDLIQTIGLAILVLYLGKYTKEQVPFFSKYFIPAPVIGGL
                     IFSIAILIGRQTGLFSINLNHGLDDFLMVLFFTCTGFMASIHVIKRSGKQGLVLAVAA
                     VTLLFLQNLLGMGVSSLMGIHPLLGVSMGSVSMSGGIGSAAAFGPSFEALGASSATII
                     GIAAATFGLVMGSLIGGPVAKRLIERMRLETSSSGTSISFSDLDQAPGTTSALSSVST
                     ILITMALGSVLVLLLNKTGITFPYYVGGVFAAALVRNLLDMTDYKINMKHIDLVGNIS
                     LALFLTLTVMSLKIWELFALALPMLTVLACQTVLMILFAYFVTFRLMGKDYEAAVMSA
                     GHCGVGLGQTPNAIANMSTVIDKYGPAPTAWIMLPVITVIIINICNPFVITLFMNLFK
                     "
     gene            complement(56172..57380)
                     /locus_tag="VFF22_01835"
     CDS             complement(56172..57380)
                     /locus_tag="VFF22_01835"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017478723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZX15594.1"
                     /translation="MTTQFLSSRLLSIQPSPSIAANALANELRAQGRDIINFTIGEPD
                     FDTPPHIIQAASAAMQQGDTHYTDTRGSLALRQAIVSKLLRDNGLDYDLGEVIAGSGG
                     KHIIYHALAATLNAGDEVIVHAPYWVSYPDIARLNAATPVIIPGDESSGFKLTAQALE
                     QVITPHSKWVILNTPNNPSGAVYSRDELLALAEVLRQHPHVLIMSDEIYEHFVYDNAC
                     HLSIVNVAPDLKSRTLIVNGASKGYAMTGWRLGFAAGPSVLITAIGKLISQTTTCPSS
                     ISQAAAVAAFAGDQAPIAQMRDIYAQRRQRMLEGLNDIAGLRCTPPQGAFYIFANVAG
                     LLGKQSPQGLRIESDTDLANYLLQEANVATVSGAAYGMSPYLRLSFSSPMDVIEEGCR
                     RISEACQKLR"
     gene            complement(57392..58054)
                     /locus_tag="VFF22_01840"
     CDS             complement(57392..58054)
                     /locus_tag="VFF22_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018929645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HZX15595.1"
                     /translation="MLPGLRIQPCLPLAPTHLVEAFRDVVTPHISDSLGRHMGARGLK
                     RYNQTGKLVGTALTVKTRPGDNLFIYKAMTLIQPGHVLVIDAQGDTLNAVIGELIKLY
                     AQKRGCVGFVVNGAIRDVASFDSTPCYALDVVHCGPYKSGPGEINVPVSIGGMLVNPG
                     DIVVGDEDGVVAFAQEHAEELLAAACTHAAHEERIKAEIATGNPQQSWLDAVLDKHGL
                     TV"
     gene            58177..59082
                     /locus_tag="VFF22_01845"
     CDS             58177..59082
                     /locus_tag="VFF22_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010175927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX15596.1"
                     /translation="MITFKQLEALYWIAELGSFETAANKLYMSQSAISKRIQELEDSF
                     GVQLFDRSKRQARLTEKGNELLEYAKELLEKRDLLVERVSSSEVLIKRFRLGVTELTA
                     LTWLPTLVERIRQIYPKVNIEPSVELSSDLFRKLGEDQLDLIIIPDVYDDARFVVTPL
                     KTVENAWMCAPHLAPARSPIGLEELGTYTVLTQGSSSGTGLVYERWLSSHNVQMPRTL
                     TSHNLLVQVGLTLSGLGISYLPKQCLSHLVDAGTLSIISTQPGLPLIRYAALYRLDRQ
                     MGLNGHVAQIAQKSCDFSHLLLNRF"
     gene            59549..60367
                     /locus_tag="VFF22_01850"
     CDS             59549..60367
                     /locus_tag="VFF22_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016780185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZX15597.1"
                     /translation="MTTTYTPFNRLLLTGAAGGLGKVLRESLRPYANILRVSDIAAMS
                     PATGAHEEVQVCDLADKAAVHQLVEGVDAILHFGGVSVERPFEEILGANICGVFHIYE
                     AARRHGVKRVIFASSNHVIGFYKQDETIDANCPRRPDSYYGLSKSYGEDMASFYFDRY
                     GIETVSIRIGSSFPEPHNRRMMSTWLSFADLTQLLERALYTPNVGHTVVYGMSANKNV
                     WWDNHLAAHLGFQPKDSSEVFRAQIDAQPMPAADDPAMVYQGGAFVAAGPFGDD"
     gene            60425..61315
                     /locus_tag="VFF22_01855"
     CDS             60425..61315
                     /locus_tag="VFF22_01855"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010656205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZX15598.1"
                     /translation="MHAELILDARNATGESPVWSAAEQALYWVDIPANRLHRWSAADG
                     QTQSWMAPQMLACIARSHNGGWIAGMQSGLFHVQAQADGQLISSLLCEVEHARTGMRF
                     NDGRCDRQGRFWAGSMLLDMGAAAPVGAMYRYSAGQTEPLNAQLSGFIVPNGLAFSPD
                     GRTMYLSDSHPSVQAIWAFDYDIDSGTPHNRRLFVDMHSHPGRPDGAAVDADGCYWIC
                     GNDAGQIHRFTPDGRLDRSLSVPVKKPTMCAFGGRNLDTLYVTSIRPAGIDLADQPLA
                     GGVFALNPGVTGLEEPYFND"
     gene            61386..62360
                     /locus_tag="VFF22_01860"
     CDS             61386..62360
                     /locus_tag="VFF22_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003254830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZX15599.1"
                     /translation="MTFKRKLLVAALPFAFCLSGLAQAAMSLKIAEIHPAGYPTVVAQ
                     ENMGKAIEAATNGELTFKMFSGGVLGSEKEVVEQVQIGAVQMTRVSLGTLGPVVPDVN
                     VFNMPFVFRDHEHMRKVIDGEIGQEILDKITNSEFNMVGLAWMDGGVRNLYTKEPVRS
                     LEDLKGMKIRVMGNPMFIETFNALGANGIAMDTGEIFSALQTGVVDGAENNPPTMLEH
                     NHFRNAKYYTLTGHLILPEPIVISKATWNKLTPEQQEIVKKYAKEAQFDERRLWDEKS
                     ASSEAKLKEAGVEFIEVDKKPFFDATAPIREKYGAPYADLIKRIEAVQ"
     gene            62427..62954
                     /locus_tag="VFF22_01865"
     CDS             62427..62954
                     /locus_tag="VFF22_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HZX15600.1"
                     /translation="MKNTLLRINDVIYMACIWVAGLSILVMSLIIPWGIFARYVLGTG
                     AGWPEPIAIMLMVVFTFIGAAASYRAGAHMAVTTLTDRMPDHWQPIITLLVQVLMAVI
                     CLFMTIWGIKLCIATWNQFMSTLPTLRVGISYAPIPLGGAITLIFILERLVFGDQSHR
                     RVVNFELIEDSKEAV"
     gene            62955..64235
                     /locus_tag="VFF22_01870"
     CDS             62955..64235
                     /locus_tag="VFF22_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002556152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HZX15601.1"
                     /translation="MDALILLGSFLVLIMLRVPVAYSLGLSALAGAWYIDIPFHAIMI
                     QIAGGVNKFSLLAIPFFVLAGAIMAEGGMSRRLVNFASVLVGFIRGGLSLVNIMASTF
                     FGAISGSSLADTASVGSVLIPEMEKKGYPREYSTAVTVSGSVQALLTPPSHNSVLYSL
                     AAGGTVSIASLFIAGIGPGLLLSAVMMGMCLYFARKRNYPKGEVIPLKQALKICVDAL
                     WGLFTMVIILGGILSGVFTATESAAIAVVWAFFVTMFIYRDYKWRDLPKLMHRTVRTL
                     SIVMILIAFAAAFGYIMTLMQIPSKITALFLTLSDNRYVILMCINFMLLALGTLMDMA
                     PLILILTPILLPVVTGIGVDPVHFGMIMLVNLGIGLITPPVGAVLFVGAAIGKVTIEN
                     TVKALLPFYAALFMVLLAVTYIPAISLWLPSVVL"
     gene            64305..65213
                     /locus_tag="VFF22_01875"
     CDS             64305..65213
                     /locus_tag="VFF22_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZX15602.1"
                     /translation="MSAAPFPRHIAVLILATLACSFAGGHIAARIAFDHDTGLLLAML
                     CRSGVTMLALIALVLWRSESLRLKPQTWGWQLLLGLLIAVQSFCIYSAVARIPVALAL
                     LVVNLAPIMLALLTWALGGPRPTARAALLMGLILFGLVLVLDLPSRLSGETQIDANWI
                     EGVLFGLTAAAVFACALWVTENRLSAMPGTVRSMLTMLVVFVASALAGAGGLLPGGMG
                     LPSAGVGWVALACLVLLYGTAFSTLFIFVTRLNIARNAPVMNMEPVAGLLFGWLILDQ
                     LLGGIQIIGGLIVVSGIVLLTYRRPA"
     gene            complement(65235..65990)
                     /locus_tag="VFF22_01880"
     CDS             complement(65235..65990)
                     /locus_tag="VFF22_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HZX15603.1"
                     /translation="MDTPNTPPRARRKHRSLAQELVTELSQRIRDGVIKRGDKLPTES
                     AIMEEQGVSRTVVREALSRLQASGLVETRHGIGTFVLDTPSPSGFRIDPATIVTLRDV
                     LAILELRISLEVESAGLAAQRRSAEQLASMREVLDVLYQSVSQAGDAVASDFQFHLLI
                     AESTGNRYFTDIMSHLGTSIIPRTRLNSARIAHDDQEHYMARLSREHEQIFEAIARQD
                     SDAARAAMRLHLTNSRERLRQAHEAAEAQAQQA"
     gene            66131..67009
                     /locus_tag="VFF22_01885"
     CDS             66131..67009
                     /locus_tag="VFF22_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011167796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HZX15604.1"
                     /translation="MTIKLLTLEDRLTRLTLAPELGGSIVNWTLRANGHALLRPSDQQ
                     ALDAGSPRRLACYPLAPWSNRIGQGGFSSPDGWFALPANSPNDPLPIHGSAWQQPWQV
                     IEQSAGEVCLQLESVVPFAYRAQQRFRLHDGCLQMHLQVTHLDSRAAWHGLGWHPYFP
                     RTAHTRLRAVAQQFWQCDQARLSSELAPLPADWNFAEAQPLPAEQIDNVFTGWDGSCE
                     IIQQDAGYQLHCRSQGTDYFILYTPTGQDFFCFEPVSHPVNAHHLPGQPGLRLLRQGE
                     TCQLSFSLHYQALPPR"
     gene            complement(67030..67335)
                     /locus_tag="VFF22_01890"
     CDS             complement(67030..67335)
                     /locus_tag="VFF22_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15605.1"
                     /translation="MRKSMQMGALGLVLIGLFGCEAAEQSAQKLAEKAEQAVVDVARE
                     AVDETVKELNKQVDEVQQSTNEWLGKPAEDAEQTEPQQPEQPKAEPNDQAATKSIEA"
     gene            67742..68440
                     /locus_tag="VFF22_01895"
     CDS             67742..68440
                     /locus_tag="VFF22_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX15606.1"
                     /translation="MAEELHFVTEEFPPFSYSRGAIASGALPEIVDATCLILQWRCQI
                     EVLPWRRALQQAEDGEVDGIFTVIQSPAREQAFFITPMLVTSGYDFYTLRSSNFHYSQ
                     PDDLRGRLIGVYGPSGTSFVLQESLKNTPNVEVKMVTNNHRLLLMLNAGRFGEQAVVV
                     LNRDVARHLIEHEYLYALRHAGHLASISYGIGFSRKKVTLAQFQAFNRGLSTLRHNGQ
                     LATILKRYGLQPAN"
     gene            68683..68895
                     /locus_tag="VFF22_01900"
     CDS             68683..68895
                     /locus_tag="VFF22_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15607.1"
                     /translation="MTKDELRAELERQAQRYKDVYGGEVITYAAQPDPERKPWRKKAN
                     LLDQAFEKELVKIEKELQSKADEASG"
     gene            complement(68953..70449)
                     /locus_tag="VFF22_01905"
     CDS             complement(68953..70449)
                     /locus_tag="VFF22_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HZX15608.1"
                     /translation="MMQTDQFVVGAGLDGLPVGQALRLANRHGLIAGATGTGKTVTLQ
                     RLIETFSDAGVAVFAADVKGDLCGLGAAGAPQGKVAERIASMPWLNHQPMAYPLTLWD
                     VHGKSGHPLRTTLSEMGPLLLGALLELTDSQQAALYAAFKVADREGLLLLDLKDLRAL
                     LAHLKSHPEVLGEDSALFTNTSSQALLRKLAGLEQQGAEALFGEPALQLEDILHPDRD
                     GRGRIHLLDASRLVHEAPKVYATFLLWLLAELFEQLPERGDADKPLLALFFDEAHLLF
                     ADTPKALQERLEQVVRLIRSKGVGVYFVTQSPGDLPDDVLAQLGLRIQHGLRAFTAKE
                     QKSLRAVADGFRPNPAIDTLTVLTELGIGEALVGTLEAKGTPAMVQRVAVAPPQSRIG
                     PLSEAERAALIQQSPLRGRYDKPIDRESAYELLTARAEQAAQQAEQSKPSAASKKAEA
                     GGIAGAAGELLGGLASQVMKTAMRQAANQIGRQLVRGLLGSLMGGKGR"
     gene            70579..72456
                     /locus_tag="VFF22_01910"
     CDS             70579..72456
                     /locus_tag="VFF22_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX15609.1"
                     /translation="MTNNLKFSHKILLAASLVVIAAFSLFTLYNDYLQRNAIRDDLES
                     YLSEMGSVTANNIQNWLSGRILLVESAAQTIQSDPSPSRVISLIEQKALLSTFAFTYL
                     GTAEGAFTMRPDAKMPDGYDPRARPWYKDAQAAGGTTLTEPYIDAATNVPIMTIATPI
                     SPVGVVGGDLNLQVLVKIINSLNFDGLGYAFLVSADGKILVHPDESLAMKSLKDIYPE
                     NTPPISSDFSEAEVDGQTRILTFAPIEGLPSVKWYVGLSIDKAKAYSMLSEFRASAIV
                     ATIIAVVLIMLLLSMLISVLMQPLNVMGKAMEDIAQGEGDLTKRLSIVSNDEFGRLAS
                     AFNQFVERIHSSIREVSSATSQVNEVAKLVVGASNSSMINSDEQASRTNSVAAAINEL
                     GAAAQEIARNAADASNQASDARHLAEDGRQVVEKTIQAMNELSGKISASCSNIETLNS
                     KTVNIGQILEVIKSISQQTNLLALNAAIEAARAGEAGRGFAVVADEVRNLAHRTQESA
                     QEIEKMIEELQVGSRESVTTMTESQRYSEESVEIANQAGARLSSVTERIGEIDGMNQS
                     VATATEEQTSVIESLNVDITEINTLNQEGVENLNATLRACGDLEQQASRLKQLVDSFR
                     I"
     gene            complement(72575..72760)
                     /locus_tag="VFF22_01915"
     CDS             complement(72575..72760)
                     /locus_tag="VFF22_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15610.1"
                     /translation="MTDPLARGTSSAPPVLGEGCTQRYDPEALTAEDGTEFTDAAELW
                     RQLQQDEQPATNSDKQK"
     gene            complement(72788..73213)
                     /locus_tag="VFF22_01920"
     CDS             complement(72788..73213)
                     /locus_tag="VFF22_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZX15611.1"
                     /translation="MLKSIKVRDYMTRNLVTFRPEMNLFTAINRLLEHRISGAPVVDG
                     QGHLVGLLSEGDCLRGILSGAYYESAGGDVSAYMTTEVEVISPEADIIEISERFLRGR
                     RRRLPVVEEGRLIGQVSRSDVLRAVKEFAQHDEGRLALS"
     gene            complement(73259..74110)
                     /gene="purU"
                     /locus_tag="VFF22_01925"
     CDS             complement(73259..74110)
                     /gene="purU"
                     /locus_tag="VFF22_01925"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HZX15612.1"
                     /translation="MRTFRLVIACPDRVGIVAKVSNFLATYNGWITEASHHSDNQSGW
                     FFMRHEIRADSLPFDLEGFRQAFAPIAREFSMEWRVTDSAVKKRVVLMASRESHCLAD
                     LLHRWHSDELDCDIPCVISNHDDLRSMVEWHDIPYFHVPVNAQDKQSAFDEVARLVKA
                     HAADVIVLARYMQILPAPLCAEFSQRVINIHHSFLPSFVGAKPYHQASLRGVKLIGAT
                     SHYVTEELDAGPIIEQDVVRVSHRDSIEEMVRLGKDVEKMVLSRGLRYHLEDRVLVHD
                     NKTVVFD"
     gene            74413..74787
                     /gene="mvaT"
                     /locus_tag="VFF22_01930"
     CDS             74413..74787
                     /gene="mvaT"
                     /locus_tag="VFF22_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein MvaT"
                     /protein_id="HZX15613.1"
                     /translation="MSLINEYSATEEAIKELQDRLKNLSQDDKLKKELEFEGKLRALM
                     GEYQKSLRDIIAMLDPDAKGSKVTRATKTTGTKRARKVKQYKNPNTGEVIETKGGNHK
                     TLKEWKAKWGGDVVEGWATLLG"
     gene            complement(74890..76320)
                     /gene="sbcB"
                     /locus_tag="VFF22_01935"
     CDS             complement(74890..76320)
                     /gene="sbcB"
                     /locus_tag="VFF22_01935"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017940004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HZX15614.1"
                     /translation="MTSSLFWYDYETTGIDPRRDRPLQVAGIRTDEDLNEIAEPLNIY
                     CQPSDDILPHPAACLVTGITPSTLAQHGLDEAEFMSRVHAELSQPGTCGVGYNSLRFD
                     DEVTRYSLYRNFFDPYAREWQGGNSRWDLIDLVRTAYALRPEGIVWPEEDGRVTLKLE
                     RLTAANGIEHGQAHDALSDVRATIALARLLRIKQRKLYDYLYQLRSKQRVQDQIRLLQ
                     PVVHISGRYAGARHYLAVVLPLAWHPRNRNALIVCDLQADIAPLLNESAETLRTRLYT
                     RRDQLAEGELPVPLKLLHINRCPVVAPMNVLREEDIQRLQLDVPAYQAQAEQLRINPG
                     LWQDKLAQVYAEEQFAANQDPEQQLYDGFIGDRDRRLCEQVRRAEPQRLAEAVWPFDD
                     GRLPELLFRYRARNFAATLSAEEQQRWWTFCQQRLSQPEYGAPNTLEQFSQALAAALP
                     DLGAEQQAILLEWSDYAEQLRQRYAL"
     gene            76490..77251
                     /locus_tag="VFF22_01940"
     CDS             76490..77251
                     /locus_tag="VFF22_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15615.1"
                     /translation="MSDISPSNPYAAPASALQEAPRDSQVPSIEEALSRGYDFNISDL
                     LSEAWQRVKGTKGIIIGGFLVFYAVLLAASFLIGGFFGLLGVVSDSPMLAVVAELVIG
                     ILASALAYPFMAGINMIGIRRAADQPISFNDIFSHFGRTVPLVITAIISMLLIYLGMF
                     LLIIPGLYLAIAYMLAIPLVVERGLTPWQALEASRKAITQHWFKVFGLFLLLGLITLV
                     SAIPLGIGLVWTIPLFVIAMGVLYRTIFGVLPAPQ"
     gene            77353..78075
                     /locus_tag="VFF22_01945"
     CDS             77353..78075
                     /locus_tag="VFF22_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZX15616.1"
                     /translation="MSPTPTPASVAARPLLDTRYKVETPEGIDLILRPAGVVPRALAF
                     SIDLLIRGALLGALFAVLALLGQFGMGLSTILLFLVTWWYMVLFEVLNQGRSPGKQMM
                     GLRVIHDDGTPIGWAASLTRNLLRFVDILPFGYTLGILSCLNHPAFKRLGDIAAGTLV
                     VYRDNAPAAPELPQAEALRAPFSMSLNEQRALLSFAERAQSLSSARRQELASILAEPL
                     QVPPEQAEQQIHGIARGLLGPT"
     gene            78072..79052
                     /locus_tag="VFF22_01950"
     CDS             78072..79052
                     /locus_tag="VFF22_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005733469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HZX15617.1"
                     /translation="MKQSLFEQHHQADWQAFNAQLEALERGKADSQQCKAFAANYRSL
                     CQHLALAQSRGYSSHLIEQLQLLAMRGHQQFYRHRSPLGGQLLGFILAGFPRLVRAEW
                     RFVAAASLLFFGSLLLMGSLVYHFPDLVYGVMAPDQVAGMEKMYDPDARRIGRFSERD
                     SGDDWMMFGYYIMNNIGIAFQTFASGLLFGLGSLFFLLFNGLMIGAVAGHLTQIGYGE
                     TFWSFVVGHGAFELTAIALAGAAGLKLGWALLVPGRLPRGEALRVAAGSSVRLVGGVI
                     VFLLLAAFIEAYWSSMDLASPLVKYWVGAGLWLLVLAYLLLAGRGVHAPD"
     gene            79039..80616
                     /locus_tag="VFF22_01955"
     CDS             79039..80616
                     /locus_tag="VFF22_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HZX15618.1"
                     /translation="MRLTDASVAIRPRSAWEAVDLGVLLAKQHAGLLMASWALVTVPI
                     FALLCLLLWDYPSIALLIFWWLKPAYERLPLYILSHALFGSTPSLKQALKALPGLLKP
                     QLLASLTWRRLSPTRSFDLPVQQLEGLSGKARSQRLIVLGQRDAGGASWLTIVGMHLE
                     MALWFGIISFVYMLLPAQMELDWTWDSLVNAASGEWLWLEHLSNLIYVLLLILWEPIY
                     VACGFTLYLNRRTALEAWDIELVFRRLRQRLTGVAYALLLGCGVLLMQAPNSAMAAAP
                     VSSCPLPIEDPIGPQAERLLKQPLTSLGAQESISQLLDQPPFEHRETVTRWRLGDEPK
                     EPREEDSKALIELLEKFFKLTEMWKSVDAVALFFEVLLWAALFSLIAWLLWRYRDWLS
                     TFAGRLRLPQGRTYQPPEQLFGLEVAPQSLPADIPGEVERLWDEQPRAALGLLYRALL
                     SRMLHEQRLPLKSSHTEAEVLQLVQALQQKDLSHFSQALTRHWQNLAYGHRLPPAALK
                     QGLCNAWRRLFEQGVQA"
     gene            80613..81866
                     /locus_tag="VFF22_01960"
     CDS             80613..81866
                     /locus_tag="VFF22_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HZX15619.1"
                     /translation="MNRRIQFLLGAGLLLVLGLLAIYLLGKLTPYQETLEHGPDPEAQ
                     ANPYLAAEHFLRKQGLQVQRADGLEVMKDLPSAGQTLLLLGDRNRMTPKQAERLLAWA
                     AKGGHLLFVAERLWDEEEGKSGDLLLDSLGIQQYESEELDEDASEATDEETSTEDETN
                     AEQQAEPMPDEEAPAAADEGNAYPELTQLYLENEQAPAYFNFDTDFHLYDAENRAHAW
                     ANSDAATHLLQLYHGDGLISVVSDSWIWQTESIGEYDNAWLLWYMTQDSQVTLLYRAD
                     RDNLFSLLLEHFPQALVSLALLLAFGLWHLGMRQGPLLLPASRNRRQLQEHLRGSADF
                     LLRHNGQHSLLQGLQQDIQRRARHRHPGFERLAVAEQWQVLSRLTRQPSSVISQAMRP
                     LPKQRLAAADFTRQVANLQTLRNAL"
     gene            81863..82954
                     /locus_tag="VFF22_01965"
     CDS             81863..82954
                     /locus_tag="VFF22_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005889118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZX15620.1"
                     /translation="MSEHTPEQPESAIEPAAVTTEPVTEAPAAAPAPAAPAAAAANPN
                     AQRQRASQLAQALRAELQKALIGQTAVIDDVLSALIAGGHVLVEGVPGLGKTLLVRAL
                     ARCFGGEFSRIQFTPDLMPSDVTGHAVYDLQSEQFKLRKGPVFTNLLLADEINRAPAK
                     TQAALLEVMQERQVTLEGRALNVPLPFMVLATQNPIEQEGTYPLPEAELDRFMLKLRI
                     DYPQQSEELNMVRQVARSSKADMLEVTPLRTLLQARDVLALQKIASDLPIDEQVLDYA
                     VRIARATRTWPGLAIGAGPRASIALVRCGRARALLRGGDFVLPDDIKGCALAVLRHRV
                     RLSPELDIEGLSVDQVLQQLLDQVPAPRL"
     gene            82995..84326
                     /locus_tag="VFF22_01970"
     CDS             82995..84326
                     /locus_tag="VFF22_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZX15621.1"
                     /translation="MKPSRALLALLAGLFALGIALGVCSALAIAVPRSFTPIYWGLLL
                     VLLGLALVDALLLRQVPSPRIERQLPGNLPLGRWSEVRLNLHHSYNRPQQVEVFDHLP
                     GDMAFEHLPLKVDLHPGEQTQVSYRVRPLLRGHFHFPRCEVSLPSPMGLWRARRYLPL
                     AAETRVYPDFARLYGAQLMAVDDWLSQLGVRQQQRRGLGQEFHQLREFRDGDTLRQID
                     WKATARKRTPIAREYQDERDQQIIFLLDCGRRMRSQDGELSHFDHALNACLLLSYVAL
                     RQGDAVGLSTFAGEQNRFLAPVKGQAQLNVLLNALYDLHSTQQPADFSAAVENLLSRQ
                     RRRALVVLVTNLRDEDDQALLGAVRRLSRQHRVLVASLREEVLDTLRHTPVQDLSSAL
                     DYCGTLDYLNARASLHERLIANKVPVLDARPSELGPQLVSSYLAWKKAGVL"
     gene            complement(84358..85095)
                     /locus_tag="VFF22_01975"
     CDS             complement(84358..85095)
                     /locus_tag="VFF22_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZX15622.1"
                     /translation="MSLFPADTVISPATPAALRELIERRRAVRRFTAEAVPDEVVRDC
                     LELAVLAPNSCNLQPWSFQVIRDPALLARLHPVCMSQNAAKAPLIIAVLARPDTWKQA
                     CQNIIDYWPEPEVPARIRSFYSKTAPFQYNQGPFGLLGLFKRQLLRVAAWRKPLMRAP
                     NSQAQMRIWAVKSTALAAQNLMLAFQSHGYATCPMEGFDEVRLRKTLSIPRQALPIML
                     LAVGKQDEKGVYNPRLRFPLEQHVTWL"
     gene            complement(85191..85673)
                     /locus_tag="VFF22_01980"
     CDS             complement(85191..85673)
                     /locus_tag="VFF22_01980"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HZX15623.1"
                     /translation="MSAFHDLNLRALDGQELPLAPFKGQVVLVVNVASKCGLTPQYAG
                     LEKLYQQYKSQGFSVLGLPCNQFAEQEPDSEEAIREFCSLNYGVTFPLGSKLEVNGPE
                     RHPLYRLLAGEGAEFPGDITWNFEKFLVGHDGRVLARFSPRTTPDDPSVIQAIEKALG
                     "
     gene            complement(85964..86449)
                     /locus_tag="VFF22_01985"
     CDS             complement(85964..86449)
                     /locus_tag="VFF22_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZX15624.1"
                     /translation="MLIAANKAVSIDYTLTNDAGEVIDSSAGGAPLAYLHGAGNIIGG
                     LEKALLGKQAGDELEVAVEPEDAYGEYSAELVATLNRSMFEGVDELEVGMQFHASGPD
                     GGMQIVTIRELEGDDVIVDGNHPLAGQRLHFKVKVISVRDASEEEVAHGHIHGEGGHH
                     H"
BASE COUNT        17622 a        27515 c        25112 g        16213 t
ORIGIN      
        1 gaatagctga tttgtctgtg cccgcttggc agccgcacaa caactgtata ggcatacaga
       61 taaaagcctg cgtagaattg cctctcaaca cagacccgag agcaacgcgg tggacttaca
      121 acagggcttt ctgctgaccc ggcactggcg tgatacgccg gcagggacgc aggtggagtt
      181 ctggctggcc accgataacg gcccacggca tatccgcctg ccctatcagc cctcggtcgc
      241 ctttatcccg gcggcgcagc gcgagcaggc cgaggcgctg ctgcaaaacg agcgcggcgt
      301 cgaactgcgc ccgctgggcc tcaaggactt tcaccagcgc ccagtgctag gcctgtattg
      361 ccagcagcat cgccagctga tggaactcga caagctgctg cgccgcgccg gcgttgaggt
      421 ctacgagacc gatgtgcgcc cgccggagcg ctacctgatg gagcgattta tcacagcacc
      481 tgtgctgttt gccggcacgc ccgacccgag tggcactggc ctgctgctgg atgcgcagat
      541 caaaccctgc acggattatc ggccgactct taagctggcc tcgctggata tcgaaaccaa
      601 tgcctttggc gagctgtatt cgattgccat cgaaggctgc ggccagcgca acgtgtatat
      661 gctcggccgc acacccgagc agccgccggc ggtggatttt cagctggagt actgcgccag
      721 ccgtggccag ctgctcgaac gcttgaatgg gtggatggcg cagcatgacc ccgacgcaat
      781 catcggctgg aacctggtgc agttcgacct gcgcgtgctg cacgaacatg cacgcaacct
      841 ggccattccg ctgcgcctgg gccgcgatgg cagtgaaatg cagtggcgcg agcatggcag
      901 ccgcaacggc cactttttcg ccgatgccgc cggccggctg attatcgacg gcatcgaggc
      961 gctgcgttcg gcgacctgga gctttccctc attcagcctg gaaaacgtcg cgcagaccct
     1021 actcggcgaa ggcaaggata tttccacgcc ctaccagcgc atggacgaaa tcgaccgcat
     1081 gttccgcgag gacaaaccgg ccctggcgcg ctacaacctg cgcgactgcg agctggttac
     1141 gcggatcttc ggcaagaccg cgatcctcga ctttctactg gagcgcgcca cggtcactgg
     1201 cttgcccgcc gaccgtaacg gcggctcggt ggcggccttt gagcacctgt atatgccgct
     1261 gatgcaccgc aggggttttg tcgcgccgaa tttgggcgac aacccaccgc aagccagccc
     1321 cggcggcttt gtcatggagt cgcagccggg gctgtacgaa tcggtgctgg tactggacta
     1381 caagagcctg tatccgtcga ttatccgcag ctttctgatc gacccggtgg ggctgatcga
     1441 aggcctgcgc cagccggacg atgcgcactc ggtgccgggc tttcgcggcg cgcgcttctc
     1501 ccgcagcgag cactgcctgc cgtccatcgt cgagcgggtg tggcagggcc gtgaggcggc
     1561 caagcgcgag cacaacgcgc cgctgtcgca ggcgttgaag atcatcatga acgccttcta
     1621 cggcgtgctc ggctccagcg gttgccgctt cttcgataca cgcctggcct cctccatcac
     1681 cctgcgcggc cacgagatca tggcccgcac ccgcgcgctg atcgaggccg aaggctatgc
     1741 ggtgatctac ggcgacaccg actccacctt tgtctggctc aagcgcccgc atgcccagga
     1801 agatgccgcg cagatcggcc gcgccctggt ggccaaggtc aatgcctggt ggcgcgagca
     1861 tttgcacaat gagtacggtc tggaaagtgc cctggaactg cagttcgaaa cccattacag
     1921 ccgctttcta atgccgacta ttcgcggcgc tgaggaaggc agcaagaagc gctacgccgg
     1981 gatggtcacg cacgccgacg gcagccatga gatggtctac aagggcctgg aaaccgtgcg
     2041 cagcgactgg tcgccgctgg cccagcagtt ccagcgcgag ctgtacgagc gcatcttcct
     2101 caaccagccc taccaggctt atgtgcgcga ctatatcgag cgcaccctga atggcgagct
     2161 ggacgagctg ctgatctatc gcaagcgcct gcgccgcaaa cttaccgact atgagcgcaa
     2221 cgtgccgccg catgtacgcg ccgcgcgcct ggccgatgaa tacaacgcag cccacggccg
     2281 cccactgcaa taccagcgcg gcggctggat cagctacgtg atcaccactg ccggcccgca
     2341 gccgctggaa gcgcgcagcg ccgccatcga ctacgaccac tacctgaccc gccagctaca
     2401 gccggtggcc gatgccatcc tgccgtttgt cgaggatgat tttgcaacct tgatcggcgg
     2461 gcagttgggg ttgttctagt agccagctct cacttattac tcgtcaggac gaccccaaat
     2521 gctgcacacc ctcgccgtcg ccaactaccg ctcgatcaac aatctggtgc tgccgctggg
     2581 ccggctcaat ctggttaccg gggccaacgg cagcggcaag tccaacctct atcgcgccct
     2641 gcgcctgctg gcggaaaccg cccagggcgg tgtggtcaat gcattggccc gtgaaggcgg
     2701 gctggactcc accttctggg ccgggccgga aacccttagc cggcgcatgc tcaacggtga
     2761 agtgccggtg caaggcggcc cgcgcaagga ggccaaacgc ctgcgcctgg gttttgccgg
     2821 cgatgatttc agctatgcca tttccctcgg gctgccggaa cccagcttgt ccgccttcgc
     2881 cctggacccc gagatcaagc gtgaatgcat ctgggccggg gccagctatc gccccgcctc
     2941 actgctggtg gatcgcgccg ggccgatggt gcgtatacgt gatggccgca actggcaggt
     3001 gatggcccag cacctgccga gtttcgacag cctgttcgat cagatcggca atgatcccgc
     3061 ctgcccggaa gtgttccagc tgcgcgaaca tatccgtcgt tggcgcttct acgatcactt
     3121 tcgcagcgat gccgaagcgc ctgcgcgcct gccgcaactg ggcacccgca ccccggtgct
     3181 gcaccacgac ggccgcgacc tggcggcggc gttgcagacc attcgtgaaa tcggcgatcc
     3241 cgccgcgctg aacgcggcca tcagcgacgc cttccccggc gcacgtctac gcatcgatat
     3301 acaggccggc gggcgcttcg ctgtcgaact gcaacaggaa ggattattgc ggccgttatc
     3361 ggccagcgaa ctgtcggacg gcaccctacg ttatctgttg ctggtcgccg ccctactcac
     3421 gccaaggccg ccatcgctga tggtgctgaa cgagccggaa accagcctgc atcccgatct
     3481 gctgcccgcc ctcggccggc tgattatcgc cgcctcccag cgcacccagg tctgggtggt
     3541 atcccacgcc agccgcctga tcgccaccct ggaacaacac cccgagtgca acgccatcgt
     3601 cctggaaaaa cagctggggc aaaccgtgat tcagggccag ggcatgctcg atgaaccggc
     3661 gtggaagtgg ccgagttaac cctggcggcc actactgcct gggaaaccag caccgccaaa
     3721 acagcatcgg aaatgtgagt ctgctcatct ctttctccaa ggggttatgg gcttgcttat
     3781 tggtggcccc ccggcaacgt acgcgcgttc agcaaatcgg cgggcctcaa ccctggcttg
     3841 cggatacacg gttcgccaac tcggaggaac tttgtcccgt tgcttcgttt ccgaacctta
     3901 aggacccaac tgcagcctac gcacccgcag ccaaccaggc atttatcagg agaaagcccc
     3961 atgagcactc cagccaagtt gcaaaccaag cagccgcaac aactgctatt cgacagcgac
     4021 ggcgtgattc ccaatagccc gctgccggtg ctgatctatc gtcaggtcga gctcgatggc
     4081 gatctggcag ccgcgttcga gcgtgtcttc gccgtcaatc aatggccacc gcagtggcgc
     4141 gccggcatct tcgattatca ccactaccat tccaacgctc atgaagcact cggcgtcgca
     4201 gctggtaacg ccgaggttat gctcggtggc gaacaaggcc aggcggtgca cctgcaggcc
     4261 ggcgacctgg tgatactgcc ggccggcacc gggcattgtc gtatcgaaca aagtgccgac
     4321 tttctcgtag tgggtgcgta ccccgcaggc caggagcagt acgatatgca gcgcgccgac
     4381 gccagcaccc atgacgcctc actggagcgc atcgcccgag tggcaacgcc gcaaaccgat
     4441 ccgctgtatg gcggccaggg cgccctggcc cacgcctggg gttgcaagcc ttaaccgaat
     4501 acccgttgac caggagattg ccatgagcat gaacgctgaa tacctcgcca acgagcccac
     4561 ccgggatgcg ctcgacgcgt tgcccggacc taccctgatt gagttcggta caccctggtg
     4621 cggccattgc caagcggcgc aacccttgct cgccgaggta ttcgccggcc atccacacgt
     4681 acgccacctt aaaatcgagg atggcagcgg ccgtcgtctc ggtcgctcgt tcaaggtcaa
     4741 attgtggcca acgctgatct tctttcacga cggccaggaa gtcgcgcgcc tggtcaggcc
     4801 caccagtgtc ggccaaattg agcaggcctt tctgacgctc accacgcctt aatccaggcc
     4861 ctcgtgatcg cggccgcgct aacccttggc ggccagtgca accgtggccg gcaacgcctt
     4921 gggtttacga aacaccagca cgttgcccag catcaccagc accaggccga acagcgccgg
     4981 ggcggtccat tgatagccct ctgcaaacac cgagatattc aacgccacca ccgggaacag
     5041 tacggtgcag taggccgcac gctccgggcc catgcgcccc accagggtca ggtaggcggt
     5101 aaagccgatc accgaaccgg gaatcgccag gtacagcaga gtgccgacgt aacgagtatt
     5161 ccactcgaaa ccgaacggcg tgccgctgac cagacagatc gccaccagca tcagcgcgcc
     5221 atagagcatg ccccaggcat tggtggtcag cggcttgagg ccggccttct gctgcagact
     5281 ggagagcata ttgccggcgg agaaacacag ggtaccgatc agcgctaggc cgatacccag
     5341 cagggtttca cgactggcct cctggccggc cagttccggc cagaacagca gggccaggcc
     5401 gcccagcccc agcgcgccac cggccagcac attgacggcg atcttctgct tgaagaacag
     5461 ccgcgcgttg atcgcattcc acagcgttgc ggtggaaaaa atcaccgcca ccaggccgct
     5521 gggtatccac tggctggcgg tgtagaagca cataaagttg atacagaaca gacacagccc
     5581 ttgcgccagg cagatcagct ggccgcgctt atcgagtttc tgcaggcgac cactaagcag
     5641 caggacggcg aacagcacgg cggcggccag ggcaaagcga taggcgatag acgcggcaat
     5701 cgccacttcg cccagttgca gcttgagggc aatccaggtg gtgccccaga tcagtacggt
     5761 caatagatag agcgacaggt tcatcaacag actcccgata gatgctaaag agtctgcgcc
     5821 tgatcagccc tgcaactctt gcacaaactt gcgcttttaa actaccaagc tcgcttttgg
     5881 atgttggata agtggcgcaa ttggcaacgc atcagcacat tgcccgcgcc catgcgcttg
     5941 accacccccg ttaaagtctt gcccacatac ctgtaggagc gggcttgtcc gcgatgcttt
     6001 tgggttgacg tcgattcgcg ggcaagcccg ctcctacacg acagcagtaa acacaggcat
     6061 agcattttca agcaaccgcc cgatgccgag gcagcccaat ggagatcagc gccgccagca
     6121 gtacaaagcc tgaagacacc cacagacagg cttccaaacc ataggcctga tacacccagc
     6181 cagacagcag cgtgccgatc agccggccga gggcgtttga catgtaatag aaacccacat
     6241 ccagcgacac gccgtcttcc ttggcgtagc tgacgatcag gtagctgtgc aacgaggagt
     6301 tcaccgcaaa cagcgcgccg aacaccatca atccgccgag caagacccac tgcgccgaaa
     6361 aatcactgtt cagacccagg gcaatggcgg ctggcaaccc ggccagcatg gcagcccagg
     6421 cgaatgccgc gcgaccatcc ggcaactggc cgcttgcctt gccggtgatg actggggcga
     6481 aggactgcac catgccgtag ccaatcaccc acgcagcgag aaagccgcca accatccaga
     6541 aatcccagcc aaacacactg gacaggtaca ccggcaacgc cacgacaaac cacacatcgc
     6601 gagcaccgaa taagaacagc cgcgccgctg acagaatatt gattgcccgg cttttggaca
     6661 gaatgtcgcg aaacttcggc ttggccttcg ccttgcctag atcctttttc aacagcagca
     6721 ggctggccag ccagaccagc gccaaaaccg ccgccatggc cagcaccgcg ccggcaaaac
     6781 cgagcagcgc cagcaacgca ccgcccataa agaagcccac gcccttgagc gcatttttcg
     6841 agccggtaag gatcgccacc cacttgtaca aggtgccctg ctggttgtcc ggcaccagca
     6901 atttgatcga gctcttggcg ctcattttgt tcaggtcttt ggcgataccg gacagcgcct
     6961 gcgcgcccat cacccaagga atggtcagcc aggctgctgg cacggtgagc atcagcaagg
     7021 ccgccacctg cagaccaagg ccgatattca tggtgcgatt gaggcccacg cgcgcgccca
     7081 ggtagccgcc gaccaggttg gtgaggacgc cgaaaatctc atagaacaga aacaacgcgg
     7141 caatctgcag cggcgtgtaa cccaggctgt ggaaatgcag caccaccagc atgcgcagcg
     7201 cgccgtcggt gagggtaaag gcccagtagt tgccggtgac cagcaggtat tggcgcacgg
     7261 caggcgagag ggtggcgaac ataagatgat cccgtgaacg gagtgccagg gtggcttggg
     7321 ctgtagggtg cgccgcgcgc accatggcgc aacaggtggt gcgcacggcg caccctacgg
     7381 gtcaaccgac catccgcgcc aactccaccg cgcggttggc gtagccccat tcgttgtcgt
     7441 accaggcgta gagcttcacc tgggtgccat tgaccaccat ggtcgacagc gcatcgataa
     7501 tcgaggagcg cgggtcggtg cggtagtcga tggataccag cggccgcgcc tcatagccaa
     7561 gaatcccagc cagcggcccg tcggcggcgg ccttgagcag tgcgttgacc tcctccaccg
     7621 tggtcgcgcg ctccacttca aatacgcagt cagtcagcga ggcattggcc aggggaacac
     7681 gcacggcgtg gccattcaac ttgccgcgca gctcggggaa gatctcggca atcgccgtgg
     7741 ccgaaccggt gctggtgggg atcaggctca tgcctgaggc acgggcgcgg cgcaggtcct
     7801 tgtgcggcgt atcgaggatg ctttgggtat tggtcaggtc gtggatggtg gtgatcgaac
     7861 cgtggcggat gccgaggttc tcgtggatca ccttgaccac cggggccagg cagttggtgg
     7921 tgcaggaggc ggcggtgacg atgcggtgct gcgtcgggtc gaacagctgc tggttgacgc
     7981 ccatcacgat attcagcgcg ccctgctctt tcactggagc acaaaccacc acgcgtttta
     8041 cgccttggtc gagataggct tgcagcaccg cgacggtttt catcttgccg ctggcttcga
     8101 tcaccagatc acagccgctc cagtcggtat cagcgatggc cttgttggcc gtgacctgga
     8161 tgcgtttgcc gtctatcact acacagtcgc catcactacc ggcctcatgc tgccagcggc
     8221 catgtaccga gtcgaaattc agcagatgcg cgtgggtcgg cgcatcgcca gccggatcgt
     8281 tgatctgcac gaattccagc tccggccagc cccaggccgc acgcagcgcc agacgaccga
     8341 tacgaccaaa accgttgata cctaccttga tagccatgga cagactctct cgttgaacgg
     8401 gttaaatatt gcgcggcgca aacataatga tcgccatacc gagcatggcc acagccgcgc
     8461 ccagcaggtc ccaatgggtc ggtcgaatgc cgtccaccag ccacaaccag ataatcgcca
     8521 tcgccacata caccccgccg taagcggcat acacgcgccc ggcggcggtc gggtgcaggg
     8581 tcagcagcca ggcgaacagc gccagcgaag cggcggccgg cactagcagc cagatgctct
     8641 tgccctgctt gagccacagg tacggcaggt agcagccgat aatctcggcc agggccgtca
     8701 aggcgaatag gcccaaggtg ttgagcatgc tcacctcagt tgccgaactg ggcgatccag
     8761 cccgcatggg ccggatgctg gatggctttc gaccgtagcg cctgcaccag gctgatggcc
     8821 tcgctgcggg cgatgcccag ctcaatcagg atgcgcgcag caatcagtcc agtacgcccg
     8881 gagccacctt tgcagtgaat cgcgatggcc ttgtcggccg ccagcagctc agcaatctgc
     8941 gccatagccg ccggccaggc tgcatcgaaa tccgcctggg gtacctgctc atcggccacg
     9001 ggtagatgca gccaaagcaa accttgcgcg gcgctcagcg cgggcaaatc gctggcggcg
     9061 ttttgcgcca gttcggcgta tggcatcagg gtcagcacgg catcggcgcc ggcctgcttc
     9121 agggtgttca gggcgctgtc gatgctgctg tccttgctgc ccgggcaagg cgtgaatatc
     9181 aaccgaccgg gacgattagg aatgctgaga acatcaaaag gatgggacat gctgagtgat
     9241 cctgagaatt aaatggaagc ctgattgacc cgtttggaca gttgctcggc ggactcctta
     9301 cgctcggagt agcggtccac caggtagtcc gtttggccgc gcagcagcag ggtgaacttg
     9361 accagttcct ccatcacgtc gaccacgcgg tcgtaatagg ccgaaggctt catccgcccg
     9421 gcgtcgtcaa attcgagaaa ggctttggcc accgaggact ggttggggat ggtgaacatg
     9481 cgcatccagc gccccagcac gcgcatctgg ttcagcgcat tgaaggattg cgagccaccg
     9541 gacacctgca tcagcgccag ggtcttgccc tgggtcgggc gcaccgcgcc catgctcagg
     9601 ggaatccagt caatctgcga cttgaacacc ccggtcatcg cgccatggcg ttccggcgag
     9661 caccagacca tgccctcgct ccacagcacc agctcgcgca gttcctgcac cttggggtga
     9721 tcctcggggg cgtcgtccgg cagcggcagg ccgctggggt tgaagatccg cgtttcggca
     9781 ccgaactgtt caagtaaacg cgcggcttcc tcagtcagca ggcgactgta agagcgcgcg
     9841 cggttggaac cgtacagcag cagaatgcgc ggtttgtgca gcggggccgg cttggccagt
     9901 tgctcctggc tgggcggatc gagcagttca gggtcgatat tcggcagttc ggggctcatg
     9961 gcctttctcc agaaaacggt ggctcagccg cacagtgagg cgcgatcagg acggtcacgc
    10021 atgctgtgca gacgcaaacg atcttcggcc aattgcgggt cgtcagcctg gagtacctgc
    10081 gccaggatct gaccaaccca ggccggcagt tgcgggtgca tacggtaata gacccactgc
    10141 ccagcgcggc ggtcctgcaa caggccgcta ctgcgcagct gggccagatg acgggagatc
    10201 ttcggctggc tttcctgcag ggcggcggtc agctcgcaga cgcacagctc gccctcctgg
    10261 gcgataagct gagtcatgcg cgcgcgggtg tcgtcggcca ggcacttaaa caaggtgacc
    10321 ggcgccagca tctgcagcgg agcctgggcc ttgctcttga ccagcacgaa catcttgatg
    10381 cgctcatgga tctgctgcag gcagtgcttg taggcattct tctgctggct gctggcgggg
    10441 tcctcaaagt cccaggcgat gtattcgcca gccccgggca aggtcctgca ctcgaaggcg
    10501 gacttgtcgc acagggtgat cacataatcg aactgctggc cgtcgaagct ctccagcggc
    10561 ttgctgtgca ggccctcggt gctcacgccc aactgctcca ggacctggcg tgcacgcggg
    10621 tcaacctcac caggcagggt gcctgcgcta aaaacctcat agccttcggg atcggtatgg
    10681 cgtagcagcg cttcggccat ctgcgagcgg gcagcattgg ccacacagag aaacagcacg
    10741 cgaatttttt ggctcatggc gatggatgtc atgcaaatac ggtttggtga atatattgat
    10801 atccgtatat cttgtaaacc gtatattcat cccacccagg tcgatccaga tcaatacttc
    10861 gcgcggcgga cctggctaga ctcgctattc gacccaagga atccagcatg aaccaggccc
    10921 ttgaatcctc agcccctcag accggatcgc cgttaggctt gttcgagcgt tatctgacgc
    10981 tctgggtctt tctctgcatc ggcggcggca ctctgctcgg cctctatgcg ccggaggcgg
    11041 ctcaggcggt gggcgcgctg gaaatcgccc aggtgaatat cccggtcggc ctgctgatct
    11101 gggtgatgat catccccatg ctgatgaaga tcgacttcag cgccatcggc gaggtctacc
    11161 agcagcgcgc cggcctgggc gtgacgctga gcatcaactg gctgatcaag ccgttcacca
    11221 tggcctttat ggcctggctg tttctacgtc atgtgtttgc agactggctg cctgccgagc
    11281 agatcgacag ctacgtggcc gggctgattc tgctcggtgc cgcgccttgc accgccatgg
    11341 tgtttgtctg gagcaacctg tgtaagggca acgccaactt caccctgacc caggtggcga
    11401 tcaacgacct ggtgatggtg ttcgccttcg cccctatagt cgccctgctg ctcggcgtgt
    11461 cgtcgatccc ggtgccctgg gacaccctgt tgctgtcggt ggtgatgtat atcgttatcc
    11521 ccctggcgat tgcccaattt atccgcgcgc gcctgctgcg ccatggtgat gcggcgttgc
    11581 aggcggtgct tacgcgcatc ggcccattct ccatcctcgc gttgctggcc accctggtac
    11641 tgctgttctc cttccagggc cagaccatcg ttgcgcaacc tttgatcatc gccatgctgg
    11701 cagtgccgat tctgctacag accctattta ttgctgcgct gggctactgg atgtgccgcc
    11761 aactcaaggt gcgccacgat atcgccggcc cggcagcgat gatcggtgcg tcgaacttct
    11821 tcgagctggc ggtggcggtg gccatcgccc tgtacggctt caactccggc gcagcgctgg
    11881 ccaccgtggt cggcgtgctg atcgaggtgc cggtgatgct ctggctggtg cgcatggtca
    11941 ataacagcaa gggctggtac gagcgcaccc aggtgcaagg ctaagaatgg aactgctcaa
    12001 atggctgaat gaccaactgc tgcgcatgga ctggctggcc tggctggtgc gtgtgctgct
    12061 ggaggatggc ttcgacctgg acatgaccag ccgcattggt gccagcctgc acttctttat
    12121 ctatgacgta atcaagatct ttatcctgct ggcggtgctg atcttcagca tttcctgggt
    12181 gcagagctat ttcccgcccg aacgcacgcg acggattctc ggcggcatga gtggcttcaa
    12241 ggccaaactg accggcgcac tgcttgggac catcacacca ttttgttcct gctcgtcgat
    12301 tccgctgttt atcgggttta ccagctcggg gctgccgctg ggagtgacct ttgcctttct
    12361 gatttcctca ccgctggtgg acctggcctc ggtgattctg ctggcgagca tcttcaactg
    12421 gtcgattgcc atcgcctacg tactgatcgg cctggtgctg gcggtgctgg gcggcacgtt
    12481 gatcggccgg gcgaagatgg agcgttacgt cgaagccttt gttatgcaca acccggtgct
    12541 cgacattccc caggccgaac tgacgcggcg tgatagggcg caattcgcct gggcccaggt
    12601 cagcgatatt ttccgccggg tctggctgta tgtgctgatt ggcgtgggca tcggtgcggc
    12661 catccacaac tggattccag cggactggat cgaagcgctg ctggggcaga acaactggtg
    12721 gtcggtgccg ctggccaccc tgctcggcat gccgatgtat gccgatatct tcggcaccct
    12781 gccgattgcc gaagctctgg tcgccaaggg catcggcctg ggtacggcgc tggcctttat
    12841 gatggccgtc accgcgctgt cgctgccctc gctgatcatg ctcaagaaag tggtcaaggc
    12901 accgctgctg gccttgttct tcggcatcgt tacggtcggc attgtgctga ttggctatct
    12961 gtttaacgcg ttcgcctacc tgtttatctg actaatggag tacgccatga tcatcaaaat
    13021 tctcggttcc ggctgcaaga agtgcatcac cctgacagaa aacacccagg ccgccctggc
    13081 caatctgggc cgcgaggcac aggtcatcaa ggtcaccgac ttcgccaaaa tcgccgccca
    13141 tggcgtgatg tcgacgcctg ccctggcgat tgacgacaag gtggtctcgg tcggcaaggt
    13201 gctgacagtc ggcgaagtgg aaaaactgct cagcgcctga gccgtctatt gcggccaccc
    13261 gcttgcacaa tcttgcgcat ttctccagcc gggggctggc acgcgggcat gcccggcgta
    13321 gcatggagcg acggaggatt tatgtcgcaa cttgagcacc tacaggtttt ccagagcatg
    13381 cacagctcgc ccaacacccg cctgcaccgt agcgccgagt tgggcgacgg gctggcggct
    13441 gccgtgtgga gcaaccgcca tgatgcccgc gactacgaag cccccagtca tcacacgctg
    13501 tcctgctacc tggccggcgg caccggcacc tttcgccgcg aaagcccgca cagcaaaggc
    13561 gcaccggaca agctgtgcat cctcccggcc ggccatcagt cggaatgggt gatcaacggt
    13621 gaaatccatc tggcccatct gtatatcagc caggagcagt tcgccctcgc ggcggtcaac
    13681 ctgctggacc gtgagccacg ggaactggaa ctgcgcgaag gcacctttct cgatgacccg
    13741 cagcaggccc agcgctttcg tcagctcagc cagctggatt ggcaggaacc ggccgaacgc
    13801 ctgctcagca gcagcctggc tcatggcctg atcaatcacg cgctactgtc ccaggtgggt
    13861 cgccgcgaag gcctgcgcct gaccggcggc ctggccccta gccagcgccg ccgggtgcgc
    13921 gagtttatcc aggccaatct cagtgaggcg ctcagcctgg gtcagctggc cagtttgtgt
    13981 gcgctctccg aatatcactt cgcccggatg ttccaactca gcttcggcct gccgccgcac
    14041 cgttatgtgc tgtcgcaacg cctgcgccat ggccagcaac tgctgcaaca cagcacgctg
    14101 gcactggctg aggtggccct ggcctgcggc tttgccagcg ccagccactt caacaaccgt
    14161 ttccgtcagg cgttcagcac aacgccgggg caataccgcg ccgccctgca acgcccctga
    14221 gcccacgcca agcagcatca gcccgccgca tacccgccta tcaggcaacg cagcttgcgc
    14281 cccctgagcc tgaaaacagc ctgctaatcc cgcgctggct gtgcaagaat catcggctgc
    14341 cttcgagaaa gctgcccatg ccacgattca tccactcgcc tgcccggtgc cactgatggc
    14401 cctcgatatc ctgcatctac gccaggaaga ctggcgcgcg gaattcggcg tgggcgatct
    14461 gcagcgcggc cagggctatg ccgcgcaagg ccgcagccgt ttgctcagtc tcaaggacag
    14521 caccctgttg gccagctgcc gtggctccgg tgcgcaaacc tatcaacagc gcatcaccct
    14581 gcatcattac ggccagggct ggggcgtgac cggccattgc agctgcccgg tgggcttcaa
    14641 ctgcaaacac gtggccagcg ccctgctcac cctgcaaacc cagcaggaac aaggcgtcga
    14701 cctctcgccg ctgatcgtca gcagcccgca ggcccgcgaa gagtgcctgg aaaacctgca
    14761 acccagcccg gtactgagcc tcggcagcct ggtgcgcgtg cactttgatg cacgcaaggg
    14821 gcgtatgcag gagcagaccc agcaccgcgc cgccctggcc ttcgactacc agggccacct
    14881 ggcatctggc aaacagggca aggacctgct gatcaagcag agcgcccagc acagcctgcg
    14941 catcgtgcgt gatatcagcg ctgaaaccca gctgcgcaaa cgcctggagc agaccggcct
    15001 gcaagtggca ctacggcaga gcgaagcgct gccggaaagc gccggcgaag cttacgagca
    15061 acccaacgag accgcctggc tgcagtttgt ccgcgagcaa ttgccgctgc tgcgtgagga
    15121 aggctggcag atccgcatgc agccggactt ccagtacaac ctgacaccag tggatgactg
    15181 gtatgccgag gtcgaagaag accccgagca gaactggttc gacctggagc tgggcatcga
    15241 ggtcgaaggc cagcgcatca gcctgctgcc gattctgctg caggccatcc gccgcgcgcc
    15301 ctggctgctc aacggcgagg cgctgaacaa gcgcgatgac gatgaggtgc tgctggtcag
    15361 cctgccgcac aaccacaagc gcgtggcttt gccgctggcg cgactgaaac cgctgctggc
    15421 gaccctcggc gaactgttcg tgcgtgagcc tggcgagtcc agccagcgtc tgcgcctctc
    15481 gcgcctggat gccgcgcggc tgaatcacct gcaacaaggc ccggcaatca gctggcaagg
    15541 cggcggccag ctgcgtgact tcgccgaacg cctgcagcgg ctggacagcg ccaccttgca
    15601 agcgcctgcc ggcttagaag cagagctacg gccctatcag ctgcaaggtc tggcctggat
    15661 gcagggcctg gccgagctgg aagtcggcgg cttgctggcc gatgacatgg gcctgggcaa
    15721 aaccctgcag acccttggcc atatcctctg cgagttcaat gccgggcgtt tgcagcagcc
    15781 ggcactgatc gttatgccca ccagcctgat tcccaactgg caggacgaag ccgcgcgctt
    15841 caccccgcag ctcaaggtgt tggccctgca cgggccgaag cgccgcgcgc tgttcaagca
    15901 gatcgacgag caccagatca tcctcaccac ctacgccctg ctgccgcgcg acctcaaggc
    15961 attgagcgct taccgctatc gcctgttgat tctggatgaa gcgcagaaca tcaagaaccc
    16021 gcgcagcaaa gctgccgtcg ccgctgggca actgcaggcc gggcagcgcc tgtgcctgac
    16081 cggcacgccg ctggagaatc acctcggcga gctgtggtcg ctgttcaact tcctcatgcc
    16141 cggctggctg ggtgacagca aaagctttac ccgcgactac cgcacgccga ttgaaaagaa
    16201 cgccaaccag cagcgcctga cccacctaac cgggcggatc aagcccttta tcctgcgccg
    16261 caccaaggag caggtggcca gcgagctgcc gccgaaaacc gaaatcaccc actgggtcga
    16321 actcagcgcg gcgcagcgag accgttacga aaccctgcgc ctggccatgg accagaaggt
    16381 ccgtgcggaa atcgcccgcc agggtttggc acgcagccag atagtgattc tcgaagcgct
    16441 gctgcgtcta cgccaggcct gctgcgacct gcgcctgctc ggcgatgacg gcgacagcag
    16501 cctgaccagc gcggattccg gaaagctcag cggcctgctg gaaatgcttg aagagttatt
    16561 tgccgaaggc cggcgggtgc tgctgttctc gcagttcacc tcgatgctgg cgctgataga
    16621 agccgaattg aaggcgcgca acattcccta cgccaagctc accggcagca cccaggatcg
    16681 ccgcaccccg gtgcagcagt tccaggctgg cgaactgccg atctttttga tcagcctgaa
    16741 agccggcggc tcaggcctga acctgaccgc cgccgatacg gtgatccact tcgacccctg
    16801 gtggaacccg gccgccgaag cccaagccag cgaccgcgcc taccgcatcg gccaggacaa
    16861 gccggtgttc gtctacaaat tgatcgcccg cggcagcgtg gaagagaaaa tccagcaact
    16921 gcagcaggcc aaagccaact tggcccgcgg cgtgctggaa ggcggtagcc agacccagct
    16981 gcagctcagt gaggacgacc tgcaggcgct gtttgcgccg ttaagcgact gatgcctcag
    17041 tcatcgctct gcgcatcatc acgctcggcc gcctcatccg ccgcattggt ctttttccgc
    17101 gcaccgccgg cctgcaccgc cggaaagcgc gaggaggcat agcgcaccac aaagatcgcc
    17161 agcgccagca acagaatcgc cccggagacc atcaccacgc ccatgtcggg tttatggttg
    17221 tgcgagatat cggaaatcat cagacgggtc agcgcggtaa tcgccacata gatcatgaag
    17281 cggatcggca tatggttggt cttgaaataa atccccacca tcgctgccaa ttccaggtag
    17341 ataaacaaca gcaggatgtc atctaccgtg atatggcctt tgtccaacat gccaagaaag
    17401 gccatgaccg ctgcccaggc ggtgatggcg ccgatggcga acagcgccag gtagtggaac
    17461 gcctccatca acagattgcc cagcgattcc gcgagcccat gcatgcgctc gcgcatcagc
    17521 tcggcccagt ttggcttacc cataaaagct cctagaagat cgaaaatgac gacagcaccg
    17581 cgcgtcatca tctaacagtg tcagtagaag aagcttgctg cgggcgaaaa agcacctgca
    17641 accgccgcgc catgctggcg gctggctgtt gcatcctgaa gaatcggcta tgttgttagc
    17701 cggcatggca attgctgctg gccaaatgca ggcgagttct ttatgctcgc aaccactgta
    17761 caaataaaca gtttattgca acccatcagc gtgaaggcgt agaggtgatg aatggccgtc
    17821 gaagtggtgt accgcagcag ccgggatttg gagcgtttat tcatggataa agccgaagcc
    17881 gaccgtcatg acaaaatgct cgaactggcc gagctgctca ccgaggtgct gcaaaaagcc
    17941 gtgccatcgc tgaatgaagc ccagggtgaa gagctgggga tttatatggc gaagaaccgt
    18001 gacatcttcg ccaaggcgtt caagaaccag cccgacgcct tgagtgaact gagcgaacct
    18061 gctgccgagt aacgccagca gcagtaaaca aaaccccagt cagtgactgg ggttttgtgt
    18121 ttataacgaa tacaaactca gccatataac aagcgctcgg caatggcatc ggcgaccctt
    18181 gccggtgagc gtttatcggc ctgggcatgg ccgaagattt cggtcaaccg ccggctgatc
    18241 tgcgtcagat gtgcggtgat cgctggcagc ggctcgccct gatgcttcag cgccacatag
    18301 atcaggccgc cggcgttgat cacatagtcc ggcgcataaa gaatcccccg tgcttgcagg
    18361 ctgtcggaga tatcggcgct ggccagctga ttgttcgccg cccccgccac tgccgagcat
    18421 cgcagctggc caactgtggt gtggttcagc acgccgccca gcccgcaggg cgcaagaatg
    18481 tcgcacgggg tggcgagcaa ggcctcgctg gccaccggcc gcgcgcccaa ttgctcgacc
    18541 gccaattgca cacggccctg atccagatcg ctgaccagca gttccacccc cgcagcatgc
    18601 agttgctcgg ccagcgcata acccacattg cccaggccct gaatcgccac acgcaagcct
    18661 tccaagttgt cgctgccgag gcgtgccagg gcggtggcac gaatgccggt aaatacaccc
    18721 atggctgtgt gcggcgaagg atcaccctcg gcagtggtgc tggtgacgtg ctgagtgtgc
    18781 tgggcgatgc agtccatatc gacgctggaa gtgccgctat ccatggccgt gatgtagtga
    18841 ccgttcaggg tttcgataaa ccggccgaag gcttcaaaca acgcaccgcg attgtcgaca
    18901 tgcgcaggac gaataatcac cgccttgccg ccgccctgtt gcaggccggc tagcgctgcc
    18961 ttgtagctca tgcctttggc cagacgcagc acatcctcaa tcgcgctttg cgtgtcggcg
    19021 tagggtaggt aacgacagcc acccagtgca gggcccaagc gactgttgtg gatggcgatc
    19081 atggcctgca gaccggtctg cgggtccacg gcgaagtgca acgactccag ccgggcggtt
    19141 tccatcatgg cgaacatgct gtggctcccg tactgatttg cacatcagta taggccgtgg
    19201 ctttgcaggc acccgcccag tggtcagttg cgatatggct cccgcacaac aatccactgg
    19261 acgaagcctc ggcgcttgac tacaaagcca gtgacatcgg gagattgtta tgaccccacg
    19321 cgccgcctgc ctcgcctgcc tggagcaaaa cccgcctgcg ctgttcgaag cagcgctatg
    19381 gattgccgca gagcaccagc ccacgctgca accggcagag gtgctgcaac gacttaacgg
    19441 tttgcagcag caggtcagcc agggcttgcc caatctgccg ctgcaggaac tagcgcaacc
    19501 gctgctgcgc aggctgagtg agctggattt tcatgaagac gacgaacgcc cgctgcggcc
    19561 aagggcggca atgcttgatc agctgttgct gcgccgtcgt ggccagccgt tatccctggc
    19621 actgctcgcg ctggagctgg ccaggcgttt ggggattccg ctgcaggggg tgaactttcc
    19681 cgggtacttt ctgctacgcg tgccgggcgc cgatcacttg ctcgacccgt gcagcggccg
    19741 gcgcctgtat acccgcgagt gccgcgaatt gctgcaacgc catttgggcc cgactgccga
    19801 attaagcgcc agccagctgc agccctgcga tgcgcgcagc atgttgcagc gtttgtcgcg
    19861 caacctgcgg caactgcatc aactggacga tgacccgctg gccgcgctgc gcgatgccga
    19921 gcgcgtgctg ctgcttggca cgccgaccct gcatgaccac ctggcccgcg cggagattta
    19981 tcaattactg gagtgcccac agggcgagcg cttcgatctg cagtgcgccc tgttgctcag
    20041 cgatgacccg gcgcagcacc tgcaactcag ccagcgtcta cgcctactca gcgccagccc
    20101 ggcgctgcac taaaaaaaag gctatgtccc aacagcgtgt tgcatccgac tattgctact
    20161 gagagtgaga tgacccggac ctgtagggtg cgccgtgcgc accagtgttc gctgcccgcc
    20221 ttggtgcgca cagcgcaccc tacccattgc cgccccagca agaaggactc aattctaaac
    20281 tccccatgca acacatcatt ggggcatagc ctaaaaaaag cccaacgcct tggccttagc
    20341 caccgcctga gtacgccgcg ccacgcccag cttgccgttg atccgccggg cgtgggtttt
    20401 caccgtgtgc agggaaataa acagctgctc ggccacttcc tgattggaca atccctgggc
    20461 gatcaaaccg agcaccgcca gttcgcggct gctcaaagca ttggcgttag tggcgggcgc
    20521 agcactcgcc gcattatcaa tctggctggc gacgcgaaac catgggcgca gataacccaa
    20581 gcgccgctgc agcgcctggc cggtctgtcg ggcctgttcg gcgcctactt tatcgtcact
    20641 ggctgcgcag gcttcggcat agctaagcca caagtcgctg agcaacgcct gccggccctg
    20701 ggctgatact tgctccagca actgccgcat aggctcgcgc acatcttcgc cctgcacacg
    20761 atccagttgt agcaataacg cctgaaagcg tgggatcaac tcggggaaat tcggcgtcgg
    20821 cagcaccgct ttaacccgct gttcataatc cagcacccgg gtcgccgcga cgcgcgcccg
    20881 cgcatagtgc ccctgggcaa tccacagatg actgctggcc agcaacaggg tgccgcggta
    20941 cagggtttcg gtgacgtgct ggcgctgcat ctgccgctcg gcctgcccaa gatgagcaaa
    21001 ggctgtggcc atatcgccct gccccatcgc caactcggcc aagccgaggt agccatgaaa
    21061 cgctcccgga tcgccacaag cgagggcatg ctcaagccca cgttgcagcg ccgcttccgc
    21121 ttcatccgca cggccctggc gcaaccgcag acgccccaag cgtagctcaa ggcgtgccag
    21181 taccggcgtg ttgcgcaagg cggccggcgc ggttgccgtc agcacccgct gcagcagcgc
    21241 ttcggcacga gcaaactcgc cacgggtctc cagcagcagc gcatggtcca actccagcaa
    21301 ggcttcaaac actgggttgc catgcaggcg cgcctgcttc aaggcttcga aactgagctt
    21361 ctgcgccacc tccagctgac cttcaccaat ggccaccagg gtcaacgccg aacgacacag
    21421 cagcgactgc gcccacgcgc tttgcggcag cccctgcagg gcatcatgca gatggctgcg
    21481 ggcatccaca gcgcagactc ggccgtaggc gatgatgccc tggatggctt gccagtgagc
    21541 gaacagctcg cgggtccgcg caccgtcact gcgcggctga aaacgtgcca gttgattgat
    21601 acaggtctgc gcttcatcca gacggccgac cagcagcaac acccaggcgt tgagcagaat
    21661 cagccgcggc gtgctgcaca gcaggctctc tggcaattcg cagcgccagc gcagaatcaa
    21721 tgccaggtcc tggccttgca atacctgttc ttcagtaaag cgctcgagaa aactgccggc
    21781 cacttctggc tgcccggcca acaaggcgtg ctccaccgcc gtacggatat cgccatggct
    21841 ggcaaaccac tggctggcat gtacatgcag ggcgctgcac ggaagattcg ctgcctgccg
    21901 ctgcagtagc cgagccagcg gcgggaacag cacataccag cccttggctg cgtccaactc
    21961 ctgaataaac acgccgcgct cgatcagcgc gcgcaaccac tgcgcgccct cgcccacacc
    22021 aaacagatga tcacacagcg ccggattgaa gcgcggcagc tgggccagtt gctggagcat
    22081 ctgcagcaac tcatccggca acccttgcag cacttcgcgc tcgacgtact cggcaagcag
    22141 ctgatcatgc tcgccgtcct gcggcgcgct cagcgctgtc ggttcggctt gggcagccag
    22201 aaggagcaaa cgcaaggccg caggccagcc ttgggtcagc tcatgtagat cgtgcgccca
    22261 ggcgctgcgg gtaggatcca gctgttgcaa ccaggcgaac acttccgctt cgctgaaggc
    22321 aagttcgcta gcccccagtt cgagtagctc gccttccagc aacaagcgcg agaggttgca
    22381 ggccggccgc cgccggctac ccaaccacca gcagatgccc ggcggggtga ccatcagcag
    22441 ttgattaaga aagtcatcga gggcgtgatt cggttgcgcc ggatagtcat tgagcatgat
    22501 ccacagcggc cgcgactcct gcgtcagggt tgccagcaac tcgccaatgc tgaggtctgc
    22561 ggggtaatca agcagcttgc ccagttgctg agccaattgc tcagcatgta ggccttcgcc
    22621 agcgcaggta agccagagta ggcggtaatt ggcggggcat tcacgggcgc agtcggccag
    22681 caatactgtt ttgccaaagc cggccgggcc gaccagcaag cgcagacgac agtcgtgcgc
    22741 cagcaggcgc tgctgcaggg atagccgagc aatatgccca ggcggcgggc gttgtacgct
    22801 gccacaggaa gacgctgcgg cgctcagcga gaatggcgct aatgcgttca tgacggctcg
    22861 ttttattgtt cttgcaggaa ccggatcatt gcaggctaaa ccgcaatggc caaggctcac
    22921 agttctatca cgacatgtga tggcgagcga taaaaaaggc ggcccgaagg ccgcccaatc
    22981 acggagtaac tctttaccaa gcggcgggtt taacgcacgc cgctattacg cagcgcggca
    23041 ggggtgaaat ccgcagcaga ggctttgaaa ccgaagttgt aagccatttt ctcttcgttc
    23101 ttcataccca gcgccaagta gcgaccagcc accaggtcat aaagcgcttc gagggtgtaa
    23161 cccggtgttt tatggttgta gtagttctgc gcatgccctt cggcaacacg ccacaactga
    23221 ccacggccgt cgtagtgatc aaccagcgca agctgccaag tgtcttcgtc gaagtacata
    23281 tggcgtttgg catagatatg gcgctcatcc gacttcaagg tcgcctcaac ttcccaaacc
    23341 cggtgcagct cataacgggt cagatcctga ttgatatggc cggcctgaag cacgtcatcg
    23401 tatttcagcg atggcgagtc caatttgtag ctgttgtagg ggatgtacat ctccttcttg
    23461 cccaccagct tccagtcgta gcgatctgga gcaccggaga acaggtcgaa gttgtcggcg
    23521 gtacgcatgc cgtcggcagc agtacccggc ccgtcatagg ccacctgcgg cgcgcggcga
    23581 acacgacgct gaccggcgtt gtagatccat gccaggcgcg gctccttcac ctggtcaatg
    23641 gtttcgtgga ccagcagtac gttaccagct agacgcgacg gcgcagtgac acgctgcttg
    23701 aagtacagca gcacgttggc cgccttggca gggtcgatat ccggcatatc cgacggaacc
    23761 gccacttcat ccgcgaactt aacaatgctg tatgaaccat tggtctgcgg agttgcctgg
    23821 gcaatgagac gctgtacgtt gccaccccgg tagcgagtga tgtggttcca aataacctca
    23881 acaccacttt tcgggatcgg gaacgcgtaa tagcggctgt ccgcgaagtt ctgcaagccg
    23941 ttacccccgt caatcgcggt ggtcttcagc gcgctctgct tggctgcggc ataaatggca
    24001 tctggcactg cagcggtgcg gtgagttgga tacaccggga ttttatagct ggagtagcgc
    24061 tgcagcatgg ccaactgccc tggggagagc ttgtcctggt attgcgcagc gttggcagcg
    24121 gtaatggtga gcagaggctg ttcattggca aacgggtccg cgaggaaccc cttgtcatct
    24181 accgcacccg cgtcagttgc caggccaccg gtccaggctg gaatgctgcc gtccgcatta
    24241 ccggccattt ccgcacccag cggggtcagg gcgctgccca gctgggcagc ttcctgctcg
    24301 ctgacggccg ccatcacgct gctggccagc aaggtcagca ccagcgctcc ggtttgcagg
    24361 attcttgtgt ttatcatcat gtacagatcc tttttgcgct gcattgatta gaagttcaca
    24421 ccaaagctca gggctacaaa gtcacggtca attgacgtgt tgtaatcacc accgaagaag
    24481 tcggtgtaac tgaggctcgc gttgtaagtg ttcttgtaat cggcatccaa gcccaagctg
    24541 acagccttgg aaccttcgtt gaaaagacca ttggggccgt aaccatcgac atcatgagac
    24601 caggacacgc tcggacgcag gttcaccccg gcgaacgcgt ttgagtactc caagatgccg
    24661 cgcatgcggt aaccccagga agtggaggtg acaaagccat cgtcattggc atcgctgtat
    24721 gcgccataga tggaatcgcg gccgtaatgc agatcagccg ttctttccag cccgccaacg
    24781 tgggcggcac cgatctcacc tacaacagtc aaacgactgg cacccagcac ttgatcaaag
    24841 aaatgggtaa aagtggtttg cacctgggtg atttctttgc gccggtaacc ggcgttttcc
    24901 gcaccaggtg tagtctcgat cgtgggggca gccgtaccgc cagtgagcgg attgaccagt
    24961 gcggtgttaa gcgccgtgct gttgatctga acgggtgcat taggccggta gctcgcctca
    25021 cccgcccacg atgtaccggt aggtaacacg gtggagaagc tcataccata gaggcggatg
    25081 tcttcgggat agctcaggaa atactgacca ttacccagcg ccgtgacttg cgctaaggaa
    25141 ggaaccgagg cgttgaaggt ggcgttgcca aagcacgcag ggaagccgcc aaatgcggag
    25201 caaagagcac ccagggtagt cgatggatca gccgctgcag gcgccccagc gaactgaccg
    25261 aacgtcgctg catcagcagt ctgggtatta aagaaaggag tacggctgtg atagttcata
    25321 aagtacgcgc cgtactccgt gttatcaccc agccagcgca atgccaggcc aaactgaccg
    25381 gagtcacggg catcgcggtc gccaccacgg ggaacgacca caccttcatt ttctatatca
    25441 aagcccaccc ccgtcgctgc ggcaaaaggt tgcaaggcac ggatacccgc aaccgcctcc
    25501 ggggtggtac tacctggaac ggtcagattg ttgtcgcagc catcggcaac gacatcggaa
    25561 ctagagaaaa aggtgccgca gttatcggca atggtctgat cccactcaag ctggtagaac
    25621 gcctccatgg acagattgtc ggtcaaggtt tgcgagacat agaacatgtt gacggggatc
    25681 aggccttcct tgatctccgc accaggacgg cggaatgcag ccgcatccac tgggttgatc
    25741 gagttaatgc tgttgccgat aaaggtgctc tcgccccagc tgaccacctg tttaccgacc
    25801 cgcacgctgc ctggtagatc ggcaatgttg taattgtggt agagaaaggc atcgagaatc
    25861 tgtgcaccgg aagactgcgc tgcttccttg cggttgtgat catcgatgtc tttgaacaga
    25921 cgactttcat ctttcagctc aaagtcatac cagtacttgc cacgcacgaa tacgccggta
    25981 tcgccgtact tcaattccag atcatgaata cccttgaaga tcttcgaaaa agtctcccct
    26041 ttcttgaagt tcaggcggtt gtcatcgccg gttgaggcct gcgccttgcc accattgacg
    26101 gtgccgatta gatctggatc ggcaccctgg gtcgaccagc tagcgccgac cgacaaggat
    26161 gaatcgaact ccccctcgat ttcaccaatg ttaaaggtca cagcctgggc cggcgcaatg
    26221 ccgcccaaag cgacagccac ggccagtgcg cagggctgga agtaggcatg tcttgttctt
    26281 gttgtcatgc gactctcctg gaaagcgggt ttttcatgta gagcccacaa gctactcagc
    26341 cgcctgatcc gggataagcg cccgaaagag gtatttgccc tgtcagccga aagagtgaat
    26401 gcgcctctaa aaagccgctt tgcagccggt ccatggagca tttcaatggt gctttatagg
    26461 gtgggtgaat ggccctgctg gctcgttgga ggtaatggcg gctccaagcg taatcatgca
    26521 gtccatatgg cagcaccggt accagggctg atcatggggg gtttgggcag aacaccgtat
    26581 cgatttaaat acatcgtaat gaaagcgaca caaagcgttc agcccgcgac gccagagcct
    26641 ttgcgcggtg ctctatagat gaattggtca tgctcgtatc gaaatcgcta cagacgctac
    26701 aagatcgaac ccctgaaaac aacttaactt attgttttta ttgataaaaa taacaatggc
    26761 acctactttg ctatggtctt tccacctttc gctcgtgccc ggcacgcgcg tccagcacat
    26821 cgcccccagc agaaagggcg agtcaccacc cttgaggagt tcacatgagc gaattgcgtt
    26881 ttaccgttga gcacgaatgg ctgcgtcagg atgccgatgg tctggttacc gtgggcatca
    26941 ctgcctatgc ccaggaagcc ctgggtgacg tggtattcgt tcagctgcca gaagcccaga
    27001 gctacgccga gggtgacgaa gtggccgtgc tggaatcggt aaaagctgcg agcaatatcg
    27061 ccatgccgct ggacggtgag gtggtcgagg tcaatgccga gctagaatcc agccctgagc
    27121 tggtcaacga agatcccctg ggcaagggtt ggttcttccg tttccgtccg gccaatgcca
    27181 gcgcggtagc cgacctgctc gaccaggccg cctacgagcg cctgctcaac gccaatgctg
    27241 acgcctgaga gattgtgatg accaagctga ccacccagaa cgaatttgtc gcccgccata
    27301 tcggcccacg tgacgccgat accgctgcca tgctcgaact gctcggctac gccagcatcg
    27361 acgcgctgac ctataacgtc atcccagaga gcatcaaggg caccagcgtg ctgggcgccc
    27421 agccgggcct gtcggaagcc gacgccctgg ccaagatcaa gaccatcgcc ggcaagaatc
    27481 agcagctcaa gacctttatc ggccagggct actacggcac ccacacgcca agcccgatcc
    27541 tgcgcaacct gctggaaaac ccggcctggt acaccgctta caccccgtac cagccagaga
    27601 tttcccaagg ccgcctggag tcgctgctga acttccagac cctgatcagc gacctcaccg
    27661 gtatgcagat cgccaacgcc tccctgctgg atgaagccac cgccgctgcc gaggccatga
    27721 ccttctgcaa gcgcctgtcg aagaacaagg ccagcaacgc cttcttcgcc tcccagcatt
    27781 gccatccaca gaccctcgac gtgctgcgca cccgtgccga gccgctgggc atcgaagtgg
    27841 tgattggtga cgaagccgcg attactgatg ccagcgccta cttcggcgcg ctgctgcaat
    27901 acccggcaag caatggcgat atcttcgatt accgcgaact ggtcgagcgc ttccacgcag
    27961 ccggtgccct ggtcgccgtc gccgctgacc tgctggccct gactctgctt accgcaccag
    28021 gcgagttcgg cgccgacgtg gccctgggca gtgcgcagcg tttcggtgtg ccgctgggct
    28081 tcggtggccc gcacgccgcc tacttcgcca cccgcgacgc gttcaagcgt gatatgccgg
    28141 gccgcctggt cggcatgtcg atcgaccgtt tcggcaagcc ggccctgcgc ctggccatgc
    28201 agacccgcga gcaacatatc cgccgcgaga aggccaccag taacatctgt accgcccagg
    28261 tgctgctggc caacatcgcc agcatgtacg ccgtgtacca cggcccgaaa ggcctgaccc
    28321 agatcgccca gcgcgtacac cagttcaccg cgatcttggc caaaggcctg cgcgccctgg
    28381 gttacagcgt tgagcaggaa ttcttcttcg acaccctgac cctggccacc ggcggcaaaa
    28441 ccgctgccct gcacgctgct gcgcgcaagg ccgggatcaa cctgcgtgaa gtcgacagcg
    28501 agcgtctggg cctctccctg gatgaaacca gcgaccaggc cgcagtcgaa gcccagctgg
    28561 cagtattcgc cgagggcaag gccgtaccag ccttcgcgga actggcggcc ggtgtaatcg
    28621 cccagctgcc aaaaggcctg ctgcgcgaaa gcgaaatcct cactcaccca gtgttcaatc
    28681 gctaccacag cgaaaccgag ctgatgcgct atctgcgcaa gctggccgac aaggacctgg
    28741 cgctggaccg cagcatgatc ccgctgggct cttgcaccat gaagctcaac gccgccagcg
    28801 aaatgatccc ggtgacctgg gccgagttcg gcagcctgca ccccttcgcc ccggccgaac
    28861 agagccaggg ttacacccaa ctgaccactg aactggaagc catgctctgc gcggctaccg
    28921 gttacgacgg catctccctg cagccgaacg ccggctccca gggcgagtac gcgggtctgt
    28981 tggctatccg tgcttaccac ctgagccgtg gcgatgatca gcgcgatatc tgcctgatcc
    29041 cgcaatcggc ccacggcacc aacccggcga ccgccagcat ggccggtatg cgcgtggtgg
    29101 tgactgcctg cgacagcagc ggcaacgtcg atatcgctga cctcaaggcc aaggccgaag
    29161 agcacaaaga gcgtctggcc gcgatcatga tcacctaccc gtcgacccac ggcgtgttcg
    29221 aggaaggcat ccgcgagatc gcccagatcg tccacgacaa cggcggccag gtgtatatcg
    29281 acggcgccaa catgaacgcc atggtcggcc tgtgcgcccc aggcaagttc ggcggtgacg
    29341 tgtcgcacct gaacctgcac aagaccttct gcatccccca cggcggtggc ggcccaggcg
    29401 tcggcccgat tggcgtgaaa gagcacctga ttccgttcct gcctggccac ggccatatgg
    29461 cccgcaagga aggggccgtc agcgccgcgc cgttcggcag cgccagcatt ctgccgatca
    29521 cctggatgta tatcagcatg atgggcggcg aaggcctcaa gcgcgcctcg cagatggcca
    29581 tcctcaacgc caactacatc gcccgccgcc tggaagagca ctaccccgtg ctgtactcgg
    29641 gcagcaacgg cctggtggcg cacgagtgca ttctggatat ccgtcctatc aaggacagca
    29701 gcggcatcag cgtcgacgac gtggccaagc gcctgatcga cttcggcttc cacgccccga
    29761 ccatgtcctt cccggttgcc ggcaccctga tgatcgagcc gaccgaaagc gaatccaagg
    29821 aagagctgga ccgcttctgt gacgcaatga tcgccatccg caatgagatt cgtgcggtgg
    29881 aagctggcca gttggatgcc aacgacaacc cgctgaaaaa cgccccgcac accgccctgg
    29941 aactggtcgg cgagtggacc cacgcttaca gccgcgagca ggccgtttac ccagtggcca
    30001 gcctgatcga ggccaagtac tggccgccgg tcggccgcgt ggacaacgtg ttcggcgacc
    30061 gcaacctgat ctgcgcctgc ccgtccatcg aggcgtatca ggacgcttaa gcaccgctga
    30121 aaacctcccc tcaccctaac cctctcccca gaggggcgag gggatggttc aacgtttagc
    30181 ttcacccaag aatttcaact cacctctacc cgaggcgggt tagctgcacc tcatataaaa
    30241 accagagggc ctcaccatgt tcagcaagca cgaccaactg caaggctacg atgaggccct
    30301 gctcgccgcg atcaacgcag aagagcagcg ccaggaagac catatcgagc tgattgcctc
    30361 ggaaaactac tgcagccagc gcgtgatgca ggcccagggc agcggcctga ccaacaagta
    30421 cgccgaaggc tacccaggca agcgttacta cggcggttgc gagcatgttg acaaggtcga
    30481 ggcgctggcc atcgagcgcg ccaagcagtt gttcggtgcg gactacgcca acgtgcagcc
    30541 gcactccggt tcatccgcca acagcgcggt gtacctggcg ctgctgaatg ccggcgatac
    30601 catactcggt atgagcctgg cccatggcgg tcacctgacc cacggcgcca aggtgtcgtc
    30661 ctccggcaag ctgtacaacg ccgtgcagta cggcatcgac gaacagggcc tgatcgatta
    30721 cgccgaggtc gagcgcctgg ccgtcgagca caagccgaag atgatcgtcg ccggattcag
    30781 tgcctattcg cgcaccctgg atttcgcccg tttccgcgcc atcgccgaca aggtcggtgc
    30841 cctgctgttt gtcgatatgg ctcacgtcgc tggcctggtg gccgctggtc tgtatccgaa
    30901 tccgctgccc tttgccgatg tggtgaccac caccacccat aaaaccctgc gcggcccgcg
    30961 cggcggcttg atcctggcca aggccaatga agagatcgag aagaagctca actctgccgt
    31021 attccccggc agccagggcg gcccgctgat gcatgtgatc gcagccaagg cggtgtgctt
    31081 caaggaagcg ctggagccag gcttcaaaac ctaccagcag caggtgatca acaacgccca
    31141 ggctatggcc gaagtgttcg ttgcacgcgg ctacgacgtg gtctccggcg gcaccgacaa
    31201 ccacctgatg ctggtcagcc tgatcaagca gggcctcacc ggcaaggccg ccgatgcagc
    31261 cctgggcgca gcgcatatca cggtgaacaa gaacgccgtg ccgaatgacc cgcagtcgcc
    31321 gtttgtcacc tccggcatcc gcatcggcac tccagcagtc actacacgcg gctttaagca
    31381 gggcgagtgc cgcaccctgg ccggctggat ctgcgacatc ctcgacgact tggaaaaccc
    31441 ggccgtgatc gagcgcgttc gtggtcaggt tgcggactta tgtgccagct tcccggtcta
    31501 cgctaactag ataacgcact ggttcagacg gaccgtagga gcgggccttg cccgcgaatg
    31561 tcaccgcgta tcgctggcat ggcccgctcc tacacacagc tataccgcga tcagaccaga
    31621 agtcggagca agcatgtcac ttagcgtttt tgaccttttc aagatcggca tcggcccgtc
    31681 cagctcgcac accgtcggcc cgatgctggc cgccctgcgc tttgccgaag gcctgcgccg
    31741 cgatgattta ctggccacca ccgatgccgt gaaagtggag ctatacggct ctctcggtgc
    31801 caccggcaag ggccatggca gcgataaagc cgtactgctt ggcctggaag gcgagcagcc
    31861 ggacacggtc gataccagca gcgtcgacga ccgcctggcg acgattcgca gcagcggtga
    31921 actcaatctg ctcggcgaaa aagccatccg ctttgtcgaa aaacagcatc tggcaatgat
    31981 ccgcaagccg ctggcctttc accccaacgg catgatcttt cgcgccttcg acgccgctgg
    32041 gttgcagatc cgctcacgcg agtattactc ggtgggcggc ggcttcgtgg tcgatgaaca
    32101 ggccgccggg gccgaccgca tcgtcgaaga cagcaccgtc ctgcaatacc cctttaccac
    32161 cggcaagcag ctgctggccc actgcgccga acacaagctg tcgatcagcc aggtgatgct
    32221 agccaacgag gccgcctggc gcccggaagc cgagacccgc gcgcgcctgc tgcatatctg
    32281 gcaggtgatg caggattgcg tcgaagccgg ttgccgcaac gagggcatca tgcccggcgg
    32341 cctcaaggtc aaacgccgcg ccgccgatct gcaccgtcag ctgtgcaaga accccgaagc
    32401 cgcgctgcgt gacgccctca gcgtgctcga ctgggtcaac ctctacgccc tggcggtgaa
    32461 tgaagaaaac gccagcggcg gccgcgtggt caccgcgccc accaatggag cggccggtat
    32521 cgtgccggcg gtgctgcatt actacagccg ctttatcccc agttccaacg acgacggcgt
    32581 ggtgcgcttt ctgctcaccg ccgccgccat cggcatcctc tacaaagaaa acgcctcgat
    32641 ctccggcgcc gaagtcggct gccaaggcga ggtcggcgta gcctgttcga tggctgctgg
    32701 tgccttatgt gaagtactcg gcggcagtgt caaccaggtg gaaaacgccg ccgagatcgg
    32761 catggaacac aaccttggcc tgacctgcga ccccatcggc ggcctggtcc aggtgccctg
    32821 tatcgaacgc aacgccatgg gctcggtcaa ggcgatcaac gccgcacgca tggccctgcg
    32881 cggcgacggc cagcacttcg tttcgctgga taaagtgatc cgcaccatgc gccagaccgg
    32941 tgccgacatg aacaacaagt acaaggaaac cgcccgcggc ggcctggctg tcaacatcat
    33001 tgaatgctga ttcgtagggt gcgccgtgcg caccaccccc tctaacggtg cgcacggcgc
    33061 accctaccgg agagctgtat gagcactgaa accctggcaa aaaccccact acatgccctg
    33121 catctcgaac tgggcgcacg catggtgccg ttcgccggct acgacatgcc ggtgcagtac
    33181 ccgctcgggg tgatgaagga acacctgcac acccgcgaat ccgccggcct gtttgacgtc
    33241 tcgcatatgg gtcagatcat tctgcgcggc ccgcaggcag cgcaggccct ggagcgtctg
    33301 gtgccggtgg atatcatcga cctgcccgtg ggcatgcagc gttacgccat gtttaccgat
    33361 gaccagggcg gcatcctcga cgacctgatg gtcgccaacc tgggcaacga cactttgttc
    33421 ctggtggtca acgcagcctg caagcaccag gacctgatgc acctgcaact gcagatcgcc
    33481 agccagtgcc agatcgaagc ctgtttcgac cgcgccctgc tcgccctgca aggcccgaaa
    33541 gccgtcgacg tactcgcacg cctggcaccc gaagtggcga agatgacctt tatgcaattc
    33601 gctccggtgc gcctgctcgg tgtcgactgc tacgtcagcc gctccggcta taccggcgaa
    33661 gacggcttcg aaatctcggt accagccgcc cacgccgaaa ccctggcgcg cagcctgctg
    33721 gccgaacctg aagtggccgc catcggcctt ggcgcccgcg actcgctgcg cctggaagct
    33781 ggtctgtgcc tgtatggcca cgatatgagc accagcacca cgccaatcga agccagcctg
    33841 ctgtgggcca tctccaagcc acgccgcgcc gatggcgcac gcgcgggcgg cttccccggt
    33901 gccgagcgga tcttcgccca gcaacagcaa ggcgtcgcca gcaagcgcgt cggcctgctg
    33961 ccgcaggaac gtgtaccggt acgtgaaggc gcagaaattg ttgatgccga cggcacggtg
    34021 atcggcaccg tatgcagcgg tggcttcggc ccgactctcg ccgccccagt ggccatgggc
    34081 tatctgcaga gcagccatat cgccctggat accgaggtat gggccatggt gcgcggcaag
    34141 cgcgtggcga tgaaagtggc caagacccca tttgtgccgc agcgctacta ccgcggttaa
    34201 agtgactcgt gctgcgccga ccagccggtt ggtgcagcct gattcgcgcg gttaagccgc
    34261 gcgaatatca gtaaaaatca gccgattacc aaacggatcg gtcaccgcca tatcacgtcc
    34321 ccagggctgg ctggtaatac ccggccgcgc attgccatac gcccgcgccg tcagctcggc
    34381 gtgcaaggca tccagatcat cgacactgat acgcagcgcc gcaccggggc agcaatcgcc
    34441 gtggtgttcg gacagatgca gcacgcaccc cgccctggac acttgcagat agagcggcaa
    34501 gcccgcctcg aagcgatgtg cccaatcctc gctgaaaccc aaaaactcgc agtaaaacac
    34561 ccgcgccttg gcttcatcga agctgcgcag aatgggaatc ggagccgcca gctgcataaa
    34621 cacctctgcc tgaatatgtg cccggcactc tatttcagcc ccaggcgaat gtcaccggct
    34681 cagtccggca gcaggcaatt gcgcttgttg ctgaacactt cgcggtcgat atcgcgcaca
    34741 tccagcgaca cggcattgac gctcggcagg cgctcgacca gcgcccgcac gatctgccgt
    34801 gacagcaggc gcttctgctc atccgtgcgc ccggcgagga tatagaaaat caggtgcaca
    34861 aaatcttcct gggtaccgcc cacactgaaa tgctcataca ggcttaggcg cagcttgacc
    34921 tcacccggct ggaacaactg agtggccgcg gccgcatcct cgacaacgcc gagcagttcg
    34981 gccggggtga tcaagcgcgt gagggtttgc gagccttcaa ccaggcaatg cggcatgtgt
    35041 gtctccaaaa gtatgactgg gccaaacggt gtaattcgtc atacgttatc gcacaactaa
    35101 aatcacagaa atagcgcttt tacgcggcga tcagccgctt agcgagtgcc aaccgcgcgc
    35161 cgcaagtcgc cttaatccaa cgctgaaaac tgtagaatcc cttgcatagc aaggattaca
    35221 gccaatcaag cacggctatc cggtgaataa tcgttgccgg agccatacaa ctttggtctg
    35281 gcatgcgttg acaaacccag cagaggctgt cgataatcca gccaatgttg tatgacaaca
    35341 tacaacaaat cataaaaaca aaaatcaagg tctcatcatg acgattgtcg cgctcgccac
    35401 atcgctcacc ctgtgccctg ccacagcacg ccccgcctcg ccgcccaaca ggccgagccg
    35461 ccggcgactc acgtatcgcc ccactgaatt cgccgacggc taagcctgaa gcagaggcgc
    35521 gcccccgacc aagggcgcgc ttgtcgccct ggcatggggc aactcactcg ttcctttcgc
    35581 tcgccgctgg caactcaggc cggcaaggga acggcttgtt caaaaatggg agcacaacaa
    35641 caatgactgc acgcaatgcc ctctcgctcg ccctgctcac caccgcaggc agcatggccc
    35701 tcgctctgcc actcagcagc caggctgccg gttttatcga cgactccaag gtcaacattg
    35761 gcctgcgtaa ctactacttc aaccgcaact tccttaacca cactgggccg gcggttaatg
    35821 gcgatgacca gggccaggcc gccgagtgga cgcagagcat gatcatcgat gcacgctctg
    35881 gctttaccca aggcactgtt ggttttggtc tggatgccct ggttctgggc gcgatcaaac
    35941 tcgacagcgg tggcggttcc acaggcagcg gtctgctgcc gaaacatgac gacggtgact
    36001 cggccgacag ctacgggcgc gtggccgtgg cggctaaagc catgctgtcg aaaaccgagt
    36061 tgaaagtcgg tgaatggttc gccgtgctgc cgatcctccg cgcagacgac gggcgtgcac
    36121 tgccgcaaac cttccagggt gcgcagatca cctcaaacga gttcgatggc ctgacccttt
    36181 acggtggtca gttctggaag aacagccagc gtaacgatgc cagccgtgaa gaaatgagct
    36241 acagcggcgt cgaaggcgat gatttcaact tcggcggcgg tgaatacaag ttcaacggca
    36301 acaacaccct ggtcggcgcc tggcacgccc gactggaaga cgtttaccag cagagctacg
    36361 tgcaactgac ccacagccaa ccggtcggcg actgggtgct gagcaccaac ctcggctacg
    36421 tcgacggcaa ggatgaaggc gcggccaagg ccggcaagct ggacaacaag gtctaccagg
    36481 gcagcttcac cgccaagact ggcaacaacg ccttcaccct ggcctaccag catctggatg
    36541 gcgacaccaa gttcatgcgt atcgacggtg ccagcggcgg cacgctggtc aacgacggct
    36601 tcaccaactc ctacgacaac ccggaagagc gctcctggca agttcgtcat gactacaact
    36661 tcgccggcct cggcattcct ggcatgacct tgatgaaccg ctacgtcagc ggcgacaaca
    36721 tcaaaactgc ggggcgcgat gacgccgaag agtggggccg cgaatccgag ctggcctata
    36781 ccgtgcagag cggtgcgctg aaaaacctca acgtgaaatg gcgtaactca accgtccgtc
    36841 gtgacgttgg ccaggacctg cacgaaaacc gtttgatctt caactacccg ctgtccctgc
    36901 tgtagatcca ccgcaacacc tgcatcaccg attactcctc gtgtgtgaaa ccttcagccc
    36961 gtccttgcga cgggcttttt tatgtcctgc catccgtggt ggcccacagc gagccggcgc
    37021 tgcgcgacgt taaacgtatg ccttagcgca aatcatcgac catcgccgcg acaacgctga
    37081 gcacgtcctt acccagctgc atcgaacgtt cgccgttcca gccggtgtgc ggattggggt
    37141 tgtcgtcgtg gtccttgaac ggcatctcga tggtaaaggc caggcaatcg aactccatgc
    37201 ccaccgcgtt gcaggccagg ctggtattgg cctggccgaa ctgatcgggg gtgtagccga
    37261 aggtggtctg gaattcggca ccgttatcga tcagcaactg cttgaactgg ttttccagac
    37321 gctgcaggcg agggctgtaa cccggattgc cttcgcagcc ggcgacaaac acatggggga
    37381 tttcctcgtc gccgtggatg tccagaaaca ggtcgacgcc caccgcgcgc atctgctgct
    37441 ggacgaaaaa cacctcgggg ctgtccgcct cgctggccga ctgccaggcg cggttaagat
    37501 cgcgtccagc gacgttggta cgcagatgac cacggaaggc gccgtccggg ttcatgttcg
    37561 gcaccaggta caggtcggcc ttgtccagca gggcattcag ctcggcatcg tcgcgcagct
    37621 gcaagcgctc aagcagccct tgcataaacc actcggccat atgctcaccg ggatgctgct
    37681 gggcgatcag ccagatcttg cgctgcgcct gcgggttgcg gcggatacgc agcaactcga
    37741 tggggcggcc ttcgatactc tttcccgtgg caaacagctc ggcgcctgcc aaacgctgcg
    37801 catcctcgat caactggcta tggcgctcac ggctataggg ctcgaaatag gcgaaccaga
    37861 tctgctcgtg ctgcggctca atatcgaagc tcagcgcgcc gtcggcaaag cggctaggca
    37921 cccgaaacca gtcgctctga tcgtaggagg ccacggtgtg gtaaccgctc caggcattcg
    37981 ggtaagacga ctgcccggcg ttgcccaggc tgaagccata gcgctgccca ggctgcaagc
    38041 cttcgacctt gaaatggaac cactggaaat gcccactgtt gagatccgga cgaatcgcca
    38101 gcaacacctg ctgcgggttg ctggcgtcga tgacctcgat attgccgctg tcgaagttgg
    38161 agctgatctg catggatacc tcgaaggctt gaatgcacgg agagacacga atatgaccca
    38221 gaatcgcagc tctggtgcat aggcgaaacg ctgcaggcga caaactgata tggattgtgc
    38281 aacctgcagc gcagccgatc attcgagcgc cacgcaactt gcgtgcgagc ctgcagtctg
    38341 gtgtttacct gccacaggtt caacttaagt gccaaggcca tatcaaggga gcagccatcc
    38401 gcatgaacat tgcgccatta cgccatcgtc cactgttgtt actgctctgc accgggctgc
    38461 tgggcggctg tgccagccaa ccggaacaaa gccagaccga acagcccacc gagcaagccg
    38521 atgcaccccg cgaaaacagc gtcgccgagg ccaacctgcg tagccaggcc aaccgtggcg
    38581 acctcgacag ccagttccag ctcggcagcc tgtatttcgt cggcaagcca aacaaggacc
    38641 tcaagcaggc cgagtactgg tggaagctcg ccgccgacaa aggccacgcc atggccgcag
    38701 tcagcctggc ctacctgtat accggccgcg acaacccgga tttcaacaac ccgcagcaga
    38761 tgctcaagta cctcaaccag tccgcagccg gcggcaaccc gatggcccag cacatcctcg
    38821 gcaacctcta cctgcgcggc gaacaaggcg taccgctgga cgccaaccag gcccgacgcc
    38881 tgtttaccag cgcctgccag cagaactaca gcgtcagttg cgaggcgttg cagaagctgc
    38941 cctgaacagc aaaagaaaac ggcgcacttg tggtgcgccg ctacaaccag atgtcagtaa
    39001 aacagacgta agaaaccgga tcgccgcttg tgacaacgct ccggatagga cggtgcgcca
    39061 cgcggcgaac cctcatattc aggcggggat cagagcggcg tcagcagctc gcccttgttg
    39121 ataaaggcct gcagattggc cagcaccagc tcttccatct gcaaacgggt ttcttgcgta
    39181 ccgctgccga cgtggggcaa cagcaccaca ttgggtaagg cgaacagtgc gggcggcacc
    39241 tcgggctcgt ctgcaaacac atccagccca gctccaccca gcgagccatc aagcagcgct
    39301 gcgaccagcg ccgattgctc gaccacgctg ccgcgcgcga tattcaccag aatgccctgt
    39361 ggccccagcg ccgccagcac gggcgcatcg atcagatgct gggtggccgc accaccaggg
    39421 caggccagca ccagaaaatc cgcccagtgc gccagggcca gcaggtccgc ctcaaaacca
    39481 taggggctgc tcgggtcctg gcggcgcttg tgataacgca ccggcatcgc aaaaccttcg
    39541 gcgcgccggg cgatggcctg gccgatacgg ccaaggccga cgatgccgag cttcttgccg
    39601 ctgaccttgc gcgtcagcgg gtagttgccc gccagccact gcccttggcg gacaaaacga
    39661 tcagcggcgg ccagctgccg cgccgtgtcg ataatcaggc ccatggccag gtcggcgaca
    39721 cagtcgttaa gcacatccgg cgtagtgctg ataacgatgc cgcgatcacg caacagctcc
    39781 agcggatagg ggtcgtagcc cacaccgaag ctgcaaatcg cgcgcaggtt tggcaggcac
    39841 gccaactgct cggcgctgca accgaaacgc gccgaagaca ccagcagctc gaactgcccg
    39901 ccctgctcgt acagaaaagc cagcggatcg gcctgctgcc atagcgcggt gacctcatag
    39961 cgtatggcca acgtgcggtt gaagcgttcg ctgagcgggc cgatctgtag tacttggggt
    40021 ttgttcatgt cggctcctgg gatggccttt atctcggtgg aaattggtgc gcacggcgca
    40081 ccctacagtc cgtacgcgcc tgctccgtag ggtgcgccgc gcgcaccggc attcaggtca
    40141 ccggggccgg gttgaacagg gtcaggtcgt tgtgcagcct gtgcttttcg gcccaggttt
    40201 gttgcttgcc gctggcgaca tccaggtaca gctggaagat atgccagccc atctcgtcca
    40261 gggtcatgcg cccgctggtg atctgcccgg catccacatc gatcaggtcg ggccagcgct
    40321 cggccagttg ggtgcgggtg gcgaccttga tcaccggcac catggccagg ccgtagggcg
    40381 taccgcgacc ggtggtgaag atatgcaggt tcatcccggc ggccagttgc agggtgccgc
    40441 agataaagtc gctggccggc gtggcgcaga accacaaacc cttgccgcgc acccgctcac
    40501 ccggcgagac caccccggcg atggggctgg agccagactt ggcaatcgag cccatggcct
    40561 tctcgacgat gttgttcagc ccgcccttct tgttgcccgg cgtggtgttg gcgctgcggt
    40621 cggccatgcc gcgctgcagg tagcggtcgt accagtccat ctcgcggatc agcgcatcgg
    40681 caacctcaac cgtggcggcg cgcggggtca gcaggtggat gccgtcacgc acctcggtgt
    40741 tctcggagaa catcaccgtg gcacccgcgc gcaccagcag gtcggcggcc acacccaggg
    40801 ccgggttggc agtgatgccg gaaaacgcat cgctgccgcc acactgcata cccaccgaca
    40861 actcggacgc tggcaccgtc tcacggcgac gctcgttcaa cacctgcagg cgcgcctcga
    40921 tcatctgcat gatctgctcg accatgccgg caaagccgtt gctggaatcc tgcagacgga
    40981 acagccactc ctcctcgtcc atgccattgg ttaactgatc gccatccatc aactggctgg
    41041 cctgcagctt ctcgcaaccg agactgatca ccagcgcctg gccgccgaga ttggggttcc
    41101 ggctgatgtt gtacagcgtg cggatcggca ccaccgcgtc cggcgcgttg atcgccacgc
    41161 cgcagccata gctgtgcgac agcgcgacca cgtcatcgac atacggatac ttgggcagca
    41221 gctccttgcg cacccgctcg acgcagtgat cgagcacgcc gaccacgcac tgcacggtgg
    41281 tagtcacgcc aagaatattg cgtgtaccga ccgtgccgtc cgggttgcgg aaaccctcga
    41341 aggtgtaacc ggtaagcggc tcaaccggcg ccgcggccag ggtcgccttg ggcaggttgt
    41401 ccagggccgg cggctcgggc atgcgcagct gcgcctcggt gacccagcgc ccggcggcga
    41461 tgggttccag ggcataaccg atgacctcgc cgtagcgcac cacgtcgcca cctacggcga
    41521 tgtcctgcaa ggccaccttg tggctctgcg gaataccttc aacagccacc aggccatcgg
    41581 caaagcggcc gcccttggcc acgccctgct cgttgaccac cacgccgacg ttgtcggccg
    41641 ggtgcaagcg gatatagcga ggcgaatcct gatggggaat caactgcatg gacatgttct
    41701 cctctatttc aagttggacg ccgccacggc gcggtcagcc gcagggcctg gcggctcgtc
    41761 cagctcgatc ctgtgaatac gaccgacgat aaacagataa ctgattaccg ccagcacagc
    41821 gttggcaccg acatagatca gcgccgcatc gaacgaaccg gtatggctga ccaggtaacc
    41881 gaccacgata ggcgtggtga tggccgccag gttaccgaag gtattgaaca gcccaccgga
    41941 caggccaacg atctgccgcg gcgaggtgtc ggccaccagc gtccagccga gcgagccaaa
    42001 acccttgccg aagaatgcca aggccatcag ggcgatcact gcgctatcac tttcgacata
    42061 aatgctcagc acgatgcagc tggccagcag catgccaacg acgatgggca gcttgcgcgc
    42121 cagggtaagg gaatagccac gccgcagcag accatccgac accaggccgc cgagcacgcc
    42181 gccgacaaag ccgctgatcg ctggcagcga agcggcaaaa ccggcggcga gaatggtcat
    42241 gccgcgcgcc tgcaccaggt acaccggaaa ccaggtgagg aagaaatagg taatggcgtt
    42301 gttgcagtac tggcccaggt atagaccgat cagcgtgcgc tgggtcagca gcagtttgag
    42361 ggtgcgccac tgcgaaccgc ccgcagcgga cggcagcggg gccaggtcga tcaggccgcc
    42421 gccgccttcg atatgggcga actcggccgc gcccagatgg ggatgctcac gcgggctgtg
    42481 catcaccttc aaccacacgc cacagagcag cagccccagg ccgcccagca caaaaaacac
    42541 atactgccag cccatgtcct gcaccaccca gcccatcagc ggagcaaata ccgcagtggc
    42601 ggcatattgc gccgacgtgg acatggcagt ggcagtggcc cgctcacggg tggggaacca
    42661 ggccgcgatg atccgtgcat taccgggaaa acacggcgca ccggcaaagc ctaccgccag
    42721 gcgcagaagg aacatgctgc caattgccca ggccagcggc agcatgtcga caaagccctg
    42781 caacagggtg aacagcgacc agcacagcag cgtgatgaaa tacacgcgct tggcgccaaa
    42841 gcgatcaaac agccagccac cgggaatctg cccgatcaca taggcccagc caaacgcgga
    42901 aaagatatag ccgagcatca ccgcatcgat gcccagatcc gcctgcagcg cagcaccggc
    42961 aatcgacagg ctggagcggt cggcaaaggt gaccgtggtg acgaggaaca gcatcagcag
    43021 aatggagtaa cgcacccggc ctggtttgac tgcttgcatc tggccaactc atcgagaaat
    43081 tcgcgtggaa cggcccggtg tcgttggaca ccgggccgct agaccgccgg gacgccggac
    43141 cgaggtttag cgcaccaggc aggggcgctt gttatcgaac tgccagttgg ggatcaggta
    43201 ctgcatggcc acggcatcgt tgcgcgcacc caggcccatg cccttgtaca actcgtgggc
    43261 cttggccagg gcatcccaat ccagctccac gcccagaccg ggagtcttcg gtacctgcac
    43321 caggccgcct tcgatctgca gcgggttgtg ggtcaggtac tggccgtcct gccagatcca
    43381 gtgggtgtcg atggcggtga ccttgcccgg cgcggcggct gctacgtgag tgaacatcgc
    43441 cagggaaata tcgaagtggt tgttggaatg cgagccccag gtcaggcccc actcgttgca
    43501 catctgcgcc acgcgcaccg aaccctgcat ggtccagaag tgcggatcgg ccagcggaat
    43561 atctactgac tgcagctgga tgcagtggcc catctgccgc cagtcggtgg cgatcatatt
    43621 ggtcgcggtc ggcaggccag tggcgcgtcg gaactcggcc atcacttcgc gaccggagta
    43681 accgttttcc gcaccgcagg ggtcttcggc ataggccagc acgccatgca gatcgcggca
    43741 caggtgcacg gcttcggcca gcgaccagcc gccgttcggg tcaagggtca cccgcgcttc
    43801 ggggaagcgt gctgccagag cgcgaatcgc ctcgacttcc tcggcgcctt gcagcacgcc
    43861 acccttgagt ttgaagtcgt tgaagccata gcgcgcatgc gccgcttcgg cctggcggac
    43921 gatggtctcg gggctcaacg cggtttcgtt gcgggtacgg aaccaggcgt catcggcatt
    43981 gcgctcgtcg cggtagccaa gatcggtctt gcccttatcg gcaataaaga acaggtagcc
    44041 gagcatctcc accgcatcgc gctgctggcc ttcgccaagc agggccgcaa ccggcacatt
    44101 gaggtgctgg ccgagcaggt cgagcaacgc cgcttccagc gcggtaactg cgtgaatggt
    44161 gatacgcaga tcgaaggtct gcaggccacg gccgccggaa tcacggtcgg cgaaggtctt
    44221 gcgcacctga ttaagcaggc cgttgtagtt gccaatggac tggccgacca gcagcgcgcg
    44281 ggcatcttcc agggtctggc gaatggcttc gccacccggt acttcgccaa cgccgatatg
    44341 cccggcgttg tctttgagaa taaggatgtt gcgggtaaag aatgggccat gggcgccact
    44401 caggttgagc agcatgctgt cgtggccggc aaccggaacg acctgcagct cggtgatgat
    44461 cggggcacct gaaaaggcac cggcagtgtt ctgtgtagtc atgaccgttc cctttttatt
    44521 gttaggcggg ttggctttaa acaagtacta aaaacgtagc gagcgaaggc taggcaaggc
    44581 ggaagcaggc taaaaaagcg gagtttacgt gcagtaaatg agcatttttt agactgcttc
    44641 caacgcagca tcgccgagcg cagtagtttt tagccgatgt aggtggtctt caccgttgta
    44701 tagaactcct gggcgtattt accctgctcg cggctgccgt aggacgaacc tttacgacca
    44761 ccgaacggca cgtggtaatc caccccggcg gtgggcaggt tgaccatgac cataccggcc
    44821 tgggagtgac gcttgaagtg gttagcgtac ttcagcgagg tggtgcagat accagcggac
    44881 aggccgaact cggtgtcgtt ggccatggcc agagcttcgt cgtagtcggc gactttgacg
    44941 atattggcca ccgggccaaa gatctcttcg cggctgatgc gcatatcggc agtgctgtcg
    45001 gcaaacagtg tcggcgccag gtagtagccg tcctgctcgc aggaaacgcg ctcgccaccg
    45061 cagaccaggc gtgcgccttc ctgcttgccg atttcgatat aacgcaggtc ctgttccagc
    45121 tgcgcctggg agaccaccgg accgatgtcc acgccttttt ccagggcgtt gccgaccttg
    45181 atcgacttca tccgctcggc catggccgca acaaactggt cgtagatacc ggcggtgacg
    45241 atcaaacggc tggaagccgt gcaacgctga ccggtggagt agaaggcgct ctgcaccgac
    45301 agctcgacgg cggtcttcag gtccgcgtca tcgaggatca cctgcgggtt cttgccgccc
    45361 atttccagct gcaccttggc gccacgggcc acacagttgg cggcgatgtt gcggccgaca
    45421 ccgaccgaac cggtaaagct cacgccatcg acctggctgt tctgcacgat ggcttcaccg
    45481 accacgcggc cgctacccat caccaggttg aacacgccgg ccgggaagcc cgcgcgggag
    45541 atgatttcgg caatcgccca ggcgcagccc ggcaccagat cggccggttt caacaccacg
    45601 cagttaccgt aggccagggc tggagcaact ttccaggctg ggattgcgat agggaagttc
    45661 catggggtga tcaggccgat cacgcccagc ggctcgcggg tgacttccac cgatacgccg
    45721 gtgcgcaccg atgccagcag ctcgccagcc tgacgcaggc attcgccggc aaagaacttg
    45781 aagatattgc cggcgcggct gacttcaccg atggcttcgg gcagggtctt gccctcttcc
    45841 cgagccagca gggtgccgag ttcttcacgg cgggcgagga tttcgctgcc gactttatcc
    45901 agcgcatcgc tgcgcgcctg gatgccggac gtcgaccagg ccgggaacgc ggcgcgggcg
    45961 gcagcgatgg cctgatcaac ctgggcggca tcggcctgag cgtactcgcc gatcacatcg
    46021 gacagatccg acgggctggt gttggtcgag tagctggcag cggcgaccca ttggccgccg
    46081 ataaagttgt cgtaacgcga tggattggcc acaagagcct cctgttgcga agcggcgcat
    46141 aacgcgccgc tatcagatga cggggtttat tgcgcgccct gggtgtcgat cagcgcggcg
    46201 agcatttcgt actcttccgg cagcaggtcg gtcagcggcg tgcgcacggg gccggcgtca
    46261 tgcccaacaa tcttggcacc ggccttgatg atgctgacgc cataaccagc gcaacgattg
    46321 cggatggcca ggtacggcag gaagaactcg tcgatgatct tgccaacggt ggcgtgatcg
    46381 tcacgggcca tggcgtggta gaaattcatc gcggttttcg ggatgaagtt gaacaccgcc
    46441 gaggagtaca ccggcacacc cagcgcttta taagcagcgg cgtagacttc ggcagtctgc
    46501 aggccgccga ggtagctcag gcggtccccc agacgacggc ggatcgacac catcagctcg
    46561 atatcgccga tgccgtcctt atagccgatc aggttcgggc aacgatctgc cacctgttcc
    46621 aacaatggtg cggtcaggcg gcagacgctg cggttgtaca ccaccacgcc gatcttcacc
    46681 gatttgcaca cggcttcaac gtgcagcgcc acgccttcct ggctggcttc ggtcaggtaa
    46741 tgcggcagca gcagcagacc tttggccccc aggcgctcgg cttcctgagc catttcgatg
    46801 gcctgacggg tcggaccacc gacgcccgcg agaatcggca cgctgccggc gcaggtgtcg
    46861 acggcggtct tgatcactgc cgaatactct ttgccagtca gcgagaaaaa ctcacccgta
    46921 ccaccggcgg cgaacagcgc cgaagcaccg tagggggcca gccactccag gcggcgggca
    46981 taggtatcgg cgcggaaatc gccggcagca tcgaagtcgg tcaccgggaa ggacagcaga
    47041 ccggaagaga ggatggactt cagttcttgt ggattcatta ttcgaacacc ctgtagagca
    47101 attgtgaggg gaagcgtcag ccgcagctgt agagatacgt tatcgtacaa caatcactag
    47161 cgccatacct ttacgctacc gaccatcgtc gatagccgcc ctcgcgtgcc ggccatgctc
    47221 agcgcaacaa ccaccccatt tcgctccaac cttgatctag acgcttcgct attgcgcggc
    47281 tgaaacacgg aacaagacag cggtgcaccc catgatcccg tccgtagcga aaaccagtgg
    47341 tcgctcaccg cacttttcag ccctggctgc gttgacaagc ccggcaaacg ctgctgataa
    47401 tccggccaag ttgtacgaca acgcataaca attctaaaaa caaaaatcag gccaagccat
    47461 gattacagcg acccaggccc acgtgccttt gacatgcccg accgccgcca tcccgcgaac
    47521 ttcgctgggt gtccgccagc ctaatgcatt gcgcctgcgg cgcagttcga ccttcgtctg
    47581 ccctcctgct ggccgattgc ccctgcagcg cagcgacagc ccagaacacc tcgttctatt
    47641 tgccccattg cccggcgtca tgccggcagt gccgagcacc cgattcgtta acggttaagc
    47701 ccctcactgc accgtgctgt catcaggaga accatgcgta tgtttgaaca ggccccgaca
    47761 cacacaccgc acgtggtgac gatgcaggtc attccggtgg ccggccgcga cagcatgctg
    47821 ctcaatctgt gcggcgccca tgcgccgtat ttcacccgca acctggtgct gcttaccgac
    47881 agcgccgggc gcaccggctg cggtgaagtg ccgggcggcg aaggcattcg ccaggcgctg
    47941 gaacgctgcc gcgacctggt catcggccaa cccatcggcc gttacaaccg cgtgctcaac
    48001 agcttgcgcc aagccatcgc cggacgcggc aatggcccgc agaccaccca gcatcaggtc
    48061 acctcggaat ccgaagcgcg ggtgctgcag cagccgcacg aaatcaacct gcgcctggac
    48121 aacgtgatca ccgcggtgga agccgctctg ctcgatctgc tcggccagca cctcaatgta
    48181 cccgtggccg aactgctcgg cagcggccag cagcgtgacc gcgtgccgat gctggcttac
    48241 ctcttctata taggtgagcg ccagcgcacc gacctgccct acctgacagg caagggttcg
    48301 actgacgact ggtatcacct gcgccaccag gccgcgctga cacccgacgc catcgcccgc
    48361 ctggctgagg ccgcgcatgc gcgttacggc ttcaacgatt tcaagctcaa gggcggcgtg
    48421 atgcgcggtg aggaggagat ggacgcgatt cgcgcgatca aggcgcgctt ccccgacgct
    48481 cgcgtaaccc tcgaccccaa cggcgcctgg tcgctggacg aagccattgc cctgtgccag
    48541 ggccagggcc atgtgctggc gtacgccgaa gacccctgcg gcccggagaa cggttattcc
    48601 ggtcgcgaga tcatggccga attcaaacgc gccaccggca tccccaccgc caccaatatg
    48661 gtcgccaccg actggcggca gatgggccat tcgctgcgcc tggaggccgt ggatatcccg
    48721 ctggccgacc cgcacttctg gaccatgcag ggcgccgtgc gcctgggcca ggtctgcgag
    48781 gagttcggca tgacctgggg ctcacactcc aacaaccact tcgatatttc cctggccatg
    48841 ttcacccacg ccgcagccgc cgtgccgggg cgcatcaccg ccatcgacac ccactggatc
    48901 tggcaggaag gcgaagaacg cctgacccgc gagccgctgc agatcgtcgg cggccaggtc
    48961 gaagtaccgg ataaaccggg cctcggcatc gaacccgata tgcagcagat catggccgcc
    49021 cacgagcttt acaagaaagt ggccaacggt gcgcgcgacg acgccatggc catgcaatac
    49081 ctggttccag gctggcagta ccaccccaag caaccgagcc taggccgcca accggcctag
    49141 cgttcaacgc ccccagcagc acctcaccac cggagactta caatgaaaaa aaccctctgc
    49201 gcctctctga tgtcggccat gctgaccttt actgccggca gcgccttcgc ggccgacgct
    49261 cccaactggc cgacccgtcc ggtgcaggtc gtggtcatcg ccaaccccgg cggcgacacc
    49321 gatttcaacg ctcggatgat ggccaagtac tttaaccaga tcaccggcaa gaccatggtg
    49381 gtgaccaacg tcgctggcgg cggtggcacc ctcgccgccg aacaggtcaa gggcgcggcg
    49441 gcggacggaa ataccatcct cttcacccat ccgggccagc tgatcgtcaa cgaagtggcc
    49501 ggcctgaccg aagacagcta tgagaccttc gatgttgcct gcttcgccgg cgtcgacaag
    49561 agcaccgtct tcgttgcctc caaggacgct ggcgtgaata ccatgcagga gctgatcgac
    49621 aagtcgaagg ccgctccgag cagcatcacc tacggcaccg agatgggcag cttctcccac
    49681 gcccagggcc tgatgctgga aaaactctcc ggcatcaagc tgaagatggt cgacggcggc
    49741 acggtttccg accgcgtggt cggcatgctc ggcggtcgcc tcgacctcgg cgccatcact
    49801 tatgggtcgg tgcaggacta cgtcaacggt ggccagatgg ttgccctcgg ccagcccaac
    49861 gatgcgcgta acccgctgct cggcgacgtg ccgacgctca aggaacaggg cctcgacctg
    49921 accatggaca agccctacgt agtggccttc cccaagggca ctgacccggc catcgtcgcc
    49981 aagatggccg acctgatgaa gcagatcacc gagatccccg aatacgccga ggacctgaag
    50041 aagttcaagc agccggtcgc ctacttcggc accgaggacg ccaaggcgat cctggccaag
    50101 acccgcgacg acttcatgca gttcaaggat gagttgcgcc aaggcaagtg agtcatgacg
    50161 caacgctggc cgagcgatca cgccagcgtt gcgcattcgt ttttcgaggt ttcccatgaa
    50221 cactaccgct aacaagaaag aactgatcat cggcctggcc atgaccggct gcagcctcgc
    50281 ctacctggtg atggcctacc aactgcccaa ccaccaaggc gtcgacgccg ccaccctacc
    50341 gctgctgctg gccggcttcc tgaccctgct cggtgtgctg caggtgttcg gtgcccttgc
    50401 cagcaagccc gcgcttggca ccgagcgcgc cagcgatacc cagggcgagc tgcccgccag
    50461 ccccgccgaa gcccccccgg ccagcgtcgt cgaacccaag accgtgctgc tgaccctggg
    50521 cctgatcctc ggctatatcg cgttgctcgg cccggtgggc tttccgctga tgacggtggt
    50581 ctacctctac ctgcagttcc tggtgctgac cccggtgcag cacaaggcca atcacctggt
    50641 ctacctgctg attgcagtgg tctgctcggc cgtcatcttc ctgctgttcc gccaggcctt
    50701 cgatctgatg ctgcccgccg gcctgctgaa caacttcatc tgaggatcga ccatgctcga
    50761 attactgcag caaggcttcg gtgccgtctt ctcactcaac atcatgctac tgatggccgt
    50821 cggcgtagcc atgggcatcg tcttcggtgc cgtgcccggc ctctcggcta ccatggctgt
    50881 cgccctgtgc ctgccactaa ccttcaccat gggcccgcaa gccggcctgt cgctgctggt
    50941 ggcgttgttt atcggtgcca cctcgggcgg attgatctcg gcgatcctgc tgaagatccc
    51001 cggtacaccc tcgtccatcg ccacggtgtt cgatggcggc ccgctgatgg agcaaggcca
    51061 cggggtcaag gcgctcggcg tcggcattgt gttctccttc ctcggcacca ttttcagcat
    51121 cgtcgcgctg atgtttatcg ccccacagct ggccaaggtt gcgctgcgct tcggcccgca
    51181 tgaatacttc gccatcgccg tattctccct gacgctgatc gccacgctat cggccggctc
    51241 gatgctcaag ggcctgtttg ccggcggtct gggcatcgcc gtgtccaccg tcggcatcgc
    51301 cccggtggaa gccatccgcc gcttcacctt tgatttcagc gagctcaacg gcggcttctc
    51361 catgctggcc gtgatgatcg gcatgttcgc cgtggccgag atcatcaagc tggccgaaac
    51421 cggccgccat gctgtgcaag gcaaagcccg ctcggtgagc atgaagaaca tcaagggctt
    51481 cggcttctcg ctcaaggagt tccgcgagca gctgcccaac gccgggcgct ccggcctgat
    51541 cggcctgggc atcggcatcc tccccggcat cggcgccggt acctccaatc tggtgtccta
    51601 catcatcgcc aagaagcgcg ccaaggacgg acacacctac ggcaagggca atatcggtgg
    51661 cgtggtcgcc agcgagaccg ccaacaatgc cggcattggt ggcgccatgc tgccgctgat
    51721 gaccttgggc attcccggcg atacggtgac cgcgatcatg ctcggcggct tcctgatcca
    51781 cggcatccag cccggcccgc tgctgtttat cagccagggt ccgttggtgt acaccatctt
    51841 cgccgcgctg atcgtcgcca ccttcatgat gctgtttatg gagttctacg gcctgcgcct
    51901 gtttatcaaa ctactcgacg tgcccaagca catcctgctg ccgatcatct tggtgctctg
    51961 tgtagtcggt gcctttggcc tgtccagccg cctattcgat gtctggtcga ttctgctgtt
    52021 cggcctactc ggctacggct tcgtcaaggc cggtttgccg gcggcgccgt ttatcatcgg
    52081 ctttattctc gggccgatgg ccgagaccaa tctgcgccgc ggcctgatgc tctccgatgg
    52141 caacttcgct gacttcttca ccacccccat cgccggcagc ttcctcggcc tggcgctggc
    52201 gttcatcatc tggcaggtag tcagctcgct gcgcaccaag aaaagcatgg tcagcgagat
    52261 cctgcgtacc tgatcaccac tcactggccc tctctgacca cagagggcca gcccccagag
    52321 ccccgctgac cataacgctt ttaggcgcgg tgcatgctca ttgcgaggca ggaaacccgg
    52381 ctcaggtagc ccttgaatgg tcaccattag ccgtaacaca caacggggac atagccactc
    52441 ataaaccggc cgacctgcgg ctgtggatcc atggacgctt cgcaccagct gggggggctt
    52501 gacagccctg ggatactgaa tacggcgccg cagccaacgc ggtgcatccc atcatgctgc
    52561 acaggaaggt gatccatgcg caatgaagaa ctcaaggctt tgtttgacca gcaagccgcc
    52621 ggctacgaca agcaatgggc aggaatggct cccattcgtg acgccctgca ttttttactc
    52681 aacgccctct tcgccgactt accggaaaac gcccggattc tctgcgtcgg tgtaggcacc
    52741 ggtacggaac tggcccactt ggccgagcgt ttccccggct ggtatttcac cgctgtggac
    52801 ccttcaggcg ccatgctcga catctgccgc cagcgagcgg aaaagggtgg ttttctctcg
    52861 cgctgcagtt ttcaccaggg ttacctcgac acgctgcctg ccgagcccag acacgacggc
    52921 gcgacctgtt ttctggtatc tcatttcttg ctcgacccgc aggcccgcat cgccttattc
    52981 cacgaaatct cccgccggct tagggcggat ggaatactgg caaatgtcga tctcgcctca
    53041 gacgtccaat cacctgccta cgagctgctg ctacgtagct ggatgacgct gatgtgctct
    53101 gccggcgtgc cgccggacgt attggaacga tcccgcgcgg cctacgcccg cgatgtggcg
    53161 atcctgccgg cgcagcaggt cgcaagcctg atcaaagagg ccgggttcga agcgccggtg
    53221 cagttctttc aggcggggtt gatgcatggg tggttttgca cactgaggcc gagaccaacc
    53281 tgcgccgtgg cctgatgctg tcggatggca acttcggggc gttcctcagt aaccccatcg
    53341 ccgcgtcgtt cctcgtcctg gcgctggcat gcatcatctg gcaggtggtc agctcgctgc
    53401 gcagcaagaa aaaacatggt gagtgcgatt ctgcgcacct gaacctgccc acgcccctct
    53461 cccgcctgga gggttgcttt cagcgagcga ccagcactgg gggtgcgtcc ggctcgagtg
    53521 acgcctgggc gaggggaatt ccaccatcag agcactgcaa caacccttcg cttttggcta
    53581 ccagcaaggc cccgaaaaca tcgccagtga cattcagggt cgtatgcccc atgtcgataa
    53641 cccggtatat gccggcaata acgccaacca gttcgaatgg caaccccatg agttgcagca
    53701 tcagcgtccc catgacgata ccgccaccgg gaatcccagg tgctgccatg gacaatagtg
    53761 tgccgaccat caccacattg atcatcatcg agacgctcag gtcgacgcca tagaagcctg
    53821 ccgcgaacac gaccagcact gagaggtaga ccgctgcccc catcagattg atggtggcgc
    53881 ccagcggtag catgaacgaa gttatccaac ctggaacctt aagatcctca ctagttacat
    53941 gcatgctcat tggcagcgtg gcggcgctgc tggtggtgga cagcgtaacc atccagactt
    54001 tcgaaatttt cttcagtaaa tacaatgggt tgatcttggt ataggtcacc accaccgtcg
    54061 acgtcagcag tagcacgaca agcaagccga tatagtcggc aaggatgaac tggccgactt
    54121 gattgaagat atccaagcca tagttgccca tggagtaggc catcagcgca aatatgccgt
    54181 aaggcgatag cgccatgacc atatctagaa ttttcaagat caaggttgag gcttgggtgc
    54241 atagggtctt gatgccactg gctctatcac ccagaaaaac cagtgccacg ccaaccagaa
    54301 ccgcaatcag gatgatctga ataacactgc ccttggccat ggcatcgaag atgttcgagg
    54361 ggatcatgcc gaggaggaac tgaccaatgg ttggtacgtc gcccacaggt gcaactgagt
    54421 tgccgtttcc agcgaagtcg acgccgggtt gtagcaaatt agcgacgacc agccccacta
    54481 agcccgcgag catggtcatc acggtgtata tcaccaaggt tttgccgccc acacgcttga
    54541 gttgggctac atcccccatg ttggcaatgc ctgccgacac gctgaaaaaa acgatgggga
    54601 taatcagcat ctgaatggcg cggataaagg catcgccgat cggtttgatg gctacgcccc
    54661 actcgggcgc aattagacca aacaacatgc cggatataaa tccgacaaat atttttgtcg
    54721 agagcccgaa ggacagtttg atacctttca tatccggcgc cctctaaaaa gtaactggct
    54781 tacgagtaaa gcgggatgtg tgatgctcat gatggacgcc tctttttact tggtattgtt
    54841 ctagcttctt aataggcaga tcagatgcat gccaatccct ggctttttgt tttatatgac
    54901 tatcgagcca tcatgtataa gatctatgca accaagtaga tcacttgaat agattcatga
    54961 ataaggtgat gacgaatgga ttacatatat tgatgatgat cactgtaatg acaggcagca
    55021 tgatccaggc cgtgggggct gggccgtact tgtcaataac agtcgacata tttgcaatgg
    55081 catttggcgt ttgacccagg ccaacaccac aatgacctgc cgacataaca gctgcttcgt
    55141 aatccttacc catcaatctg aacgtgacga aataagcgaa caggatcatc agaacagtct
    55201 ggcaagccag aaccgtcaac attggaagcg ccagcgcgaa aagctcccat attttcagcg
    55261 acatcaccgt cagcgtcaga aacaaggcca acgaaatatt tccaaccaga tcgatatgtt
    55321 tcatgttgat cttgtagtcg gtcatatcca gtaaattcct taccagggct gcagcaaaga
    55381 caccgccgac gtagtaggga aaggtgatgc cggttttgtt taacagcaga acaagaaccg
    55441 agcccagagc catggtgatc aatatcgtcg atacgctgct cagtgcagaa gtcgtgcccg
    55501 gtgcctggtc gagatcggag aagctgatcg aggtgccaga ggaggaagtc tccagtcgca
    55561 tgcgctcgat caggcgcttg gcaaccggac cgccaatcaa gctacccatg actagcccga
    55621 aggtagcggc tgcgatgcct atgatcgtgg ccgagcttgc acctagcgcc tcaaaggaag
    55681 gcccaaacgc cgcagccgat cctattcctc cggacataga gacagagccc atgctgacgc
    55741 ccagcagagg atgaatgccc atcaagctcg atacacccat gcccagcaga ttctgcaaga
    55801 acaataaggt tacggcggcg actgccaata ccaaaccctg tttcccactt cttttgatca
    55861 catggatact ggccataaag cccgtacagg tgaaaaaaag aaccatcaaa aaatcatcca
    55921 agccatggtt gagattgatg ctgaatagcc ctgtttgtct tccgatcaga atcgctatcg
    55981 aaaagatcag tccgccgatg accggtgcgg ggataaagta tttactgaag aatggaactt
    56041 gctctttcgt atacttgccc agataaagga caagtatggc gagtccgata gtctggatta
    56101 aatcgaggct gatattcata gggattcctt tttattcttc tggtcggtct ttttactgtt
    56161 gccaacaacc gctatcgtaa tttctggcag gcctcggata ttcgacggca tccctcctca
    56221 atcacatcca tagggcttga aaaagacagg cgtaggtaag gtgacatgcc gtatgccgcg
    56281 ccacttacag tggcgacatt ggcctcttgc agtaggtagt tcgccagatc cgtatcgctt
    56341 tcgatgcgca ggccttgtgg cgattgttta ccgagcagtc ctgccacgtt ggcaaagatg
    56401 tagaaggcac cctgtggtgg cgtacagcgc agtccggcga tgtcattgag gccctccagc
    56461 attcgttgcc ggcgctgggc atagatgtcg cgcatttgtg ctattggggc ttggtcgccc
    56521 gcgaatgcgg ccaccgccgc agcctggctg atcgaggaag ggcaggtggt agtctgcgat
    56581 atcagcttgc caattgccgt gatcaatacg ctgggaccgg cggcaaagcc gagacgccaa
    56641 ccggtcatcg cataaccttt ggaggcaccg ttgacgatca atgtccgact cttgagatcc
    56701 ggagcgacat tgacaatgga gaggtggcag gcattgtcgt acacgaaatg ctcgtagatt
    56761 tcgtcggaca tgatcaacac atgagggtgc tgacgcagta cttcggccag ggccaatagt
    56821 tcgtcgcggc tgtatacggc acccgatgga ttgttcggcg tgttgaggat gacccacttg
    56881 ctgtgtggcg tgatgacctg ctcgagtgcc tgcgcggtca gtttgaagcc gctgctttca
    56941 tcgcctggaa taatcaccgg cgtggctgca ttgaggcggg cgatgtccgg ataggaaacc
    57001 cagtaaggtg catgcacgat cacttcgtca cccgcgttca gggtggccgc gagcgcgtgg
    57061 tagatgatgt gcttgccgcc gctgcctgca atcacttcgc caaggtcata atccagacca
    57121 ttgtcgcgca agagcttgct cacgatggct tggcgcaggg cgaggctgcc gcgagtatcg
    57181 gtgtagtgag tatcgccctg ctgcatggct gcagaggcag cctggatgat gtgcggtggc
    57241 gtgtcgaagt caggttcacc aatggtgaaa ttgataatgt cacggccctg cgcccgtagt
    57301 tcgttggcca gcgcattggc tgcgatgctg ggcgatggct ggatgctcag caggcgtgaa
    57361 gagaggaatt gagtagtcat cgatcaattc ctcacaccgt aagtccgtgc ttgtccaaca
    57421 ctgcatctag ccaggattgc tgcgggttgc ctgtcgcaat ctctgcctta atccgctcct
    57481 cgtgagcggc atgcgtgcac gctgccgcga ggagttcttc ggcatgttcc tgggcgaatg
    57541 ccaccacgcc atcttcgtcg ccaacaacga tgtcgcctgg attgaccaac atcccaccaa
    57601 tgctcactgg cacgttgatc tcgccagggc cgctcttgta ggggccgcaa tgaaccacat
    57661 ccagggcata acaaggcgtg ctatcgaagc tggcaacatc gcggatggca ccattgacca
    57721 caaagcccac gcagcctcgc ttttgcgcat acagcttgat cagctcgccg atcaccgcat
    57781 ttagggtatc gccctgggca tcgattacca gaacgtggcc tggctggatc agggtcatgg
    57841 ctttgtagat aaacaggttg tcgcctgggc gcgtcttgac tgtcagggcg gtgccgacca
    57901 gcttgccggt ttggttgtag cgctttagcc cacgagcgcc catatggcgt ccaaggctgt
    57961 cactgatatg aggagtcacc acgtctcgaa atgcttccac gagatgggta ggtgcaagag
    58021 ggaggcaggg ttgaattctc aagccgggta acatttttat tctccttgat cggtatcggg
    58081 ctgatggccg tgtctcgatc ttaggaggga gaaaaagata gttttagcga tattttatga
    58141 tgcggtatga tcgatttttc tcatgagaaa gccggcatga ttaccttcaa acagcttgag
    58201 gcgctctact ggattgccga gctcggtagt tttgaaactg cggcgaacaa gctgtatatg
    58261 tcgcaatctg caatatccaa gcggattcaa gagcttgagg attcattcgg cgtgcaattg
    58321 ttcgaccgga gcaagcgtca agccaggctt acggaaaagg gcaatgaact gctggaatat
    58381 gccaaggagc ttcttgagaa aagggacctt ctggtggagc gagtcagctc cagcgaagtg
    58441 ctgatcaagc gctttcgcct aggcgtgacg gagctaacgg cactgacctg gttaccgacc
    58501 ctggtggagc gaattcgcca gatctatccc aaggtaaata tcgagccgtc agttgagttg
    58561 agcagcgatc tgttccgaaa actgggggag gatcagctcg acctcattat cattccagac
    58621 gtctatgacg acgctcggtt cgtggtcaca ccattgaaaa ctgtagagaa cgcgtggatg
    58681 tgcgcgccac atctggctcc tgcaaggtcg cctattggcc tggaagaact tggcacctac
    58741 acagtcctga ctcagggttc cagttcaggc acgggattgg tgtacgaacg ctggctgtcg
    58801 agccataacg tacagatgcc ccgcacgttg accagccaca acctgctggt gcaggtaggg
    58861 ctaacacttt cagggcttgg tatcagttat ctacctaaac agtgtctctc gcatcttgtt
    58921 gatgctggaa cactgagcat catttcgacc cagcctgggc tgccactgat ccgctacgcc
    58981 gccctgtacc gacttgatcg ccagatgggt ttgaacggcc acgtggctca gatagcccag
    59041 aagagttgtg acttctcaca ccttcttcta aatcgatttt gaatgacggc aggcattagc
    59101 cgccgctgac atggctcttc aatccataac caggcagcgg cgcggcagcg atgcgatgga
    59161 tgttctgcta tgtcataccc ctctgaaatc catgcacctt agccggcaca gccatccgga
    59221 cacatgagat acgttgtcgt acaacaaata ttaataatca acccacattc ccgccccaac
    59281 caccgaagcc tcgctgcctt ggctaacgca gtattaagta gagcctgaat aatcatctaa
    59341 acagccgctc tagagcatta aactcaaaaa atcagcgcat ccaaagcttg cttaatcgca
    59401 cattcgactc agcaatcagc cggtaattaa cgtgaatttt cgaccatagt cgcgttgaca
    59461 aaagcacgcg gcactcctga taatcgagcc aagtcatacg acaacagatg acaatccaat
    59521 aacaacaagc agagacgcag agactgccat gaccactacc tacacaccct tcaatcgcct
    59581 gctgctcacc ggggcggcag gcggcctcgg caaggtcttg cgcgaaagcc tgcggcctta
    59641 tgccaatatc ctgcgcgtct ccgatatcgc ggccatgagc cctgccacag gcgcccatga
    59701 agaagttcag gtttgcgacc tcgccgataa agcggcagtc catcaactgg tcgaaggcgt
    59761 ggatgcaatc ttgcacttcg gcggtgtatc ggtggagcgg cccttcgagg aaatcctcgg
    59821 ggccaatatc tgcggcgtgt ttcatatcta tgaagcggcg cgccggcatg gcgtgaagcg
    59881 ggtgatcttc gccagctcca accatgtcat cggtttctac aagcaggacg aaaccatcga
    59941 cgccaactgc ccgcgccgcc cggatagcta ctacggtctg tccaagtcct acggcgaaga
    60001 tatggccagc ttctacttcg accgctacgg catcgagacc gtgagcattc gcatcggctc
    60061 ctcgttcccc gagccgcaca accgccggat gatgagcacc tggctgagct ttgccgacct
    60121 gacgcagctg ctcgaacgcg ccctctacac ccccaacgtt ggccacaccg tggtctacgg
    60181 catgtccgcc aacaagaacg tctggtggga caaccacctg gccgcgcatc tgggcttcca
    60241 gcccaaagac agctccgagg tgtttcgcgc gcagatcgac gcgcaaccga tgcccgccgc
    60301 cgatgaccca gcgatggtct atcagggtgg cgccttcgtc gccgccggcc cgttcggcga
    60361 cgactgatcg cactacagcg acgcgcaaca ctcataacaa gaaccacccg gagaacgagc
    60421 tgccatgcac gctgaactga ttctcgacgc ccgcaatgcc accggtgaaa gcccggtatg
    60481 gagtgccgcc gaacaggcgc tgtactgggt cgatatccca gccaaccgcc tgcaccgctg
    60541 gagcgcggcc gacggccaga cgcagagctg gatggcgccg cagatgctcg cctgcatcgc
    60601 ccgcagccac aacggcggct ggatcgccgg gatgcaaagc ggcttgttcc acgtacaagc
    60661 ccaggccgat ggtcagctga tcagcagcct gctgtgcgag gtggaacatg cccgcacagg
    60721 catgcgtttc aacgacggcc gctgtgatcg tcagggccgc ttctgggccg gcagcatgct
    60781 gctcgacatg ggcgccgccg ccccggtcgg cgccatgtac cgctacagcg ccggccagac
    60841 cgaaccgctc aacgcacaac tgagtggctt tatcgtgccc aacggcctgg cgttcagccc
    60901 ggacggccgc accatgtacc tgtccgactc gcatccgtcc gtgcaagcga tctgggcgtt
    60961 tgattacgac atcgacagcg gcacgccgca taaccgccgg ctgttcgtcg atatgcatag
    61021 ccatcccggt cgcccggatg gcgcggcggt ggatgccgac ggctgctact ggatctgtgg
    61081 caacgacgcc gggcagatcc atcgtttcac ccccgatggt cgcctggacc gctcgctcag
    61141 cgtgccggtg aagaagccga ccatgtgcgc ctttggcggt cgcaacctgg acaccctcta
    61201 cgtcacctcg attcgtccag cgggcatcga cctcgccgat cagcccctgg caggcggcgt
    61261 gtttgccctc aacccgggtg tcactggcct ggaggaaccc tatttcaacg actgaggcgt
    61321 acccgctgcg cctcagtcat cgcaacgctg cactcccaac aacaacaata gactggagct
    61381 taactatgac tttcaaacgc aagttgcttg tcgccgccct gccctttgcc ttctgcctgt
    61441 caggcctggc ccaggccgcg atgtcgctga agattgctga aatccacccg gccggctacc
    61501 ccaccgtagt cgcccaggaa aacatgggca aggctatcga agccgccacc aatggtgaac
    61561 tgaccttcaa gatgttctcc ggcggtgtgc tgggctcgga aaaggaagtg gtcgaacagg
    61621 tgcagattgg cgccgtacaa atgacccgcg tcagccttgg caccctgggt ccggtcgtgc
    61681 cggacgtgaa cgtgttcaat atgccgttcg tgttccgtga ccacgagcac atgcgcaagg
    61741 tcatcgatgg cgaaatcggc caggagatcc tcgacaagat caccaactcc gaattcaaca
    61801 tggtcggcct ggcctggatg gacggtggtg tgcgcaacct ctacaccaaa gagccggtac
    61861 gcagcctgga agacctcaaa ggcatgaaga ttcgcgtgat gggcaacccg atgttcatcg
    61921 aaaccttcaa cgccctgggc gccaacggta tcgccatgga caccggcgag attttcagcg
    61981 ccctgcagac cggggttgtc gacggcgcgg aaaacaaccc gccgaccatg ctcgagcaca
    62041 accacttccg taacgccaag tactacaccc tgaccggcca cttgatcctg ccggagccga
    62101 tcgtgatctc caaggcgacc tggaacaagc tcacccccga gcagcaggaa atcgtcaaga
    62161 agtacgccaa ggaagcgcag ttcgacgaac gtcgcctgtg ggatgagaag tctgccagca
    62221 gcgaagccaa actgaaggaa gccggtgtcg agttcatcga agtcgacaaa aagcctttct
    62281 tcgacgccac cgccccgatc cgcgaaaagt acggtgctcc ttacgccgac ctgatcaagc
    62341 gcatcgaagc cgtgcagtaa cccgctctct ctgaagaacg tcgtggcaac ggctcaatgc
    62401 cgttgcctcg tgcgtggtga agtctcatga aaaacacgct gttacgcatc aacgacgtga
    62461 tctatatggc ctgcatctgg gttgccggcc tatctattct cgtcatgtca ctgatcattc
    62521 cttggggcat ttttgcccgt tacgtacttg gcactggcgc cggctggccc gagccgatcg
    62581 ccatcatgct gatggtggtg ttcaccttca ttggtgcggc agccagctac cgggcaggcg
    62641 cgcacatggc ggtgaccact ctgaccgatc gaatgccaga ccactggcag cccatcatta
    62701 ccctgctcgt gcaagtgctc atggcagtta tctgcctgtt catgaccatc tggggcatca
    62761 agctgtgcat cgccacctgg aaccagttca tgagcacttt gccgaccctg cgtgtgggca
    62821 tcagctacgc cccaatcccc ctgggcggcg cgataaccct gatctttatc ctcgaacgcc
    62881 tggtctttgg cgaccagagc caccgtcgcg tggtgaattt cgagctgatt gaagacagca
    62941 aggaggccgt gtaaatggat gctctgatcc tgcttggcag cttcctcgta ctgatcatgt
    63001 tgcgtgtgcc ggtggcctat tccctaggcc tgtccgctct ggcaggcgcc tggtacatcg
    63061 atattccgtt ccacgcgatc atgattcaga tcgccggggg cgtgaacaaa ttctccctgc
    63121 tggccattcc gttcttcgtc ctggccggcg cgatcatggc cgaaggcggc atgtcgcggc
    63181 gcctggtgaa cttcgccagc gtgctggtcg gctttatccg cggtggcctg tcgctggtca
    63241 acatcatggc ctcgaccttc ttcggcgcca tctccggctc gtctctggcc gataccgcct
    63301 cggtcggctc ggtgctgatc ccggaaatgg aaaagaaagg ctacccgcgc gaatactcca
    63361 ccgcagtgac tgtcagcggc tccgtgcaag cgctgctgac cccgccaagc cacaactcgg
    63421 tgctctactc gctggccgct ggcggcacgg tgtccatcgc ctcgctgttt atcgccggta
    63481 tcggcccggg cctgctgctc agcgccgtga tgatgggcat gtgcctgtac ttcgcgcgca
    63541 agcgcaacta ccccaagggt gaagtgattc cgctcaagca ggcgctgaaa atctgcgtgg
    63601 atgcgctgtg gggtctgttc accatggtga tcatcctcgg cggcatcctc tcaggggtgt
    63661 tcaccgccac cgagtcggcg gccattgccg tggtctgggc gttcttcgtc accatgttca
    63721 tctaccgcga ctacaaatgg cgcgacctgc ccaagctgat gcaccgcacg gtgcgcacac
    63781 tgtcgatcgt gatgatcctt atcgccttcg ccgccgcctt cggctacatc atgaccctga
    63841 tgcagattcc gtcgaagatc accgcgctgt tcctgacgct ctcggacaac cgctacgtga
    63901 tcctgatgtg catcaacttt atgctgctgg ccctgggcac gctgatggac atggcgccgc
    63961 tgatcctgat cctcacgccc attctgctgc cggtggtgac cgggattggc gtcgacccgg
    64021 tgcacttcgg catgatcatg ctggtcaacc tgggtatcgg cctgatcacc ccgccagtcg
    64081 gtgcggtgct gttcgtcggc gccgccatcg gcaaggtgac catcgagaac acggtcaagg
    64141 cgctgctgcc gttctacgcc gcactgttta tggtgctgct ggctgtgacc tatattccgg
    64201 ctatttcgct gtggttgccg agcgtggtgc tgtaacacgc aacaatgcgt cacgatcccc
    64261 tggagccgcc gacctggcgg ctccttgttt tgtaaggaca tagcatgtct gccgcaccct
    64321 tcccccggca catcgcggtg ctgattctgg ccaccctagc ctgctcgttc gccggcgggc
    64381 atatcgccgc ccgtatcgcc ttcgaccacg acaccggcct gctgctggcg atgctctgcc
    64441 gctccggcgt gaccatgctg gcgttgatcg cactggtgct gtggcgcagc gaatcactgc
    64501 gactgaaacc acagacctgg ggctggcaat tgctgctcgg cctgctgatc gcggtgcaga
    64561 gtttctgcat ctactcggcg gtggcgcgga ttccggtggc cctggcgctg ctggtggtca
    64621 acctcgcgcc gatcatgctg gcgctactga cctgggccct aggcgggcct cgaccaactg
    64681 cacgagcggc gttgctgatg gggctgattt tgttcggcct ggtactggtg ctcgacctgc
    64741 cttcgcggct gtccggtgaa acccagatcg atgccaactg gatcgagggc gtgctgttcg
    64801 gtctgacggc cgcggcggtg tttgcctgcg ccctgtgggt caccgaaaac cgcctgagcg
    64861 cgatgccggg cacggtacgc agcatgctga ccatgctggt ggtgtttgtt gcctcggccc
    64921 tggccggtgc cggcggcttg ctgccgggtg gtatgggctt gccgtcagcc ggcgtcggct
    64981 gggtcgccct ggcctgcctg gtactgctct acggaacggc gttttccacc ctgtttatct
    65041 tcgtcacccg gctgaacatc gcgcgcaatg cgccggtgat gaatatggag ccggtggctg
    65101 ggctgctgtt tggctggctg attctcgatc agctgctcgg cggcatccag attatcggcg
    65161 ggctgattgt ggtcagcggc attgtgctgc tgacctaccg ccggccggcc tgaagcatgg
    65221 ctggccaggg tgactcaggc ctgctgagct tgcgcctcgg cggcttcgtg ggcctggcgc
    65281 agacgctcgc ggctgttggt caggtgcagg cgcatggcag cgcgcgccgc atcggaatcc
    65341 tggcgggcga tggcttcaaa aatctgctca tgctcgcggc tcagacgcgc catgtaatgc
    65401 tcctgatcat cgtgagcaat gcgcgccgaa ttcagccgcg tgcgcggaat gatgctggtg
    65461 cccaggtgac tcatgatgtc ggtgaaatag cggttgccgg tcgactcggc aatcagcagg
    65521 tggaactgaa aatccgaggc caccgcatcg cccgcctgcg acacgctctg atacaacaca
    65581 tccagcacct cgcgcatgga cgccagttgc tcggcgctac ggcgctgcgc cgccaagccg
    65641 gccgattcaa cttccaggct gatgcgcagt tcaagaatcg ccagcacatc gcgcagggtg
    65701 acgatggtcg caggatcaat acgaaaaccg ctggggctcg gcgtatccag cacaaaggtg
    65761 ccgatgccat ggcgggtctc gaccagaccg gaagcctgca gccgcgacag ggcttcacgc
    65821 accacggtgc gactgacgcc ctgctcttcc atgatcgccg actcggtagg caatttatca
    65881 ccgcgtttga tcaccccgtc acggatgcgc tgagacagct cggtcaccag ctcctgagcc
    65941 aggctgcggt gctttctgcg ggcacgtggc ggggtgttcg gagtatccat atcgacggtc
    66001 tttctcggct gaaggctaaa acgcaatcat agcccaggcg gttgtacgat gacacactcc
    66061 ttgaatggaa attccagcac aaaggcccac tgatgaacca cggcgaatct tctctacgag
    66121 cacgcgcgcg atgacgatca aactgctgac ccttgaagac cgcctgacgc gcctgactct
    66181 ggccccagaa ctgggcggca gcatcgtcaa ctggaccctg cgcgccaacg gtcacgcgct
    66241 gctgcgcccg agtgatcaac aggccctgga tgccggtagc ccacgccgcc tggcctgtta
    66301 cccgctggca ccctggtcca accgcatcgg ccagggcggc ttcagcagcc cggacggctg
    66361 gtttgccctg ccagccaaca gccccaacga cccgctgccg atccacggca gcgcctggca
    66421 gcagccctgg caggtgatcg aacagagcgc gggcgaagtc tgtctgcagt tggaaagcgt
    66481 tgtgcctttt gcctaccgcg cgcagcagcg ctttcgcctg catgacggct gcctgcagat
    66541 gcacttgcag gtcacccacc tggactcacg cgcggcctgg catggtctgg gctggcatcc
    66601 gtatttcccg cgtaccgcac acacccgcct gcgcgccgtg gcgcagcaat tctggcaatg
    66661 cgatcaggcg cgcctatcca gcgagttggc gccattgccg gctgactgga acttcgccga
    66721 agctcaaccc ttgcccgccg agcagatcga caacgtcttt accggctggg atggcagctg
    66781 cgagattatt cagcaagacg ccggctatca gctgcactgc cgcagccagg gcaccgacta
    66841 tttcatcctc tacaccccga ctgggcagga tttcttctgc ttcgagccgg tcagccatcc
    66901 ggtcaacgcc catcacttac cggggcaacc gggtctgcgc ctgttacggc agggagaaac
    66961 ctgccagctg tccttcagcc tgcactatca agcgctgccg ccgaggtaaa tctggacgtt
    67021 cgacagcact caggcctcga tagacttcgt agcagcctgg tcattcggct cggcctttgg
    67081 ctgctcgggt tgttgcggct cagtctgttc agcatcttca gcgggtttgc ccagccactc
    67141 attggtcgac tgctgcacct cgtccacctg cttgttgagc tctttcacgg tctcatcaac
    67201 cgcttcgcgg gcgacgtcca caactgcctg ctcggctttc tcggcaagtt tctgcgccga
    67261 ttgctcagcc gcctcgcaac cgaacaggcc aatcagcacc agacccaggg cacccatttg
    67321 catcgactta cgcatcatgt tctccataca cagcctctac cggcctgttg tgccacgacc
    67381 caatggccgc cgcgttgatt gatcacccga ctgattaacc ttcaggcgag gcttagcggt
    67441 gggtgcgcaa atgtagggag gcgcgggagt ttcctcaatt gcatagagtt tgcaaggtgt
    67501 taccgagcag taaggtgcgc cgttgaaata gcaaggcgtt ttcagcaaaa cccaccacgc
    67561 actcagatag gcgcaagcag gcactaggca aatacgctta cacaaggcta ataatgcgaa
    67621 ccactaagcg cacctcagtt aatgcagcgc ctgtgagcat tcatcgcaac taaaggatta
    67681 tgtcgtgttg atacgcaatg cattgtgcgc attggcgagt ttgctctggg ttagcacttc
    67741 tgtggctgaa gagctgcact ttgtaaccga agagttcccg cccttcagtt attcacgcgg
    67801 agcaattgcc agcggcgccc taccggaaat agtcgacgcc acctgtctga tcttgcagtg
    67861 gcgctgccaa attgaagtat tgccctggcg ccgcgccctg cagcaagccg aagatggcga
    67921 agtggatggg atttttaccg ttattcaatc acccgcgcgg gagcaggcat tttttattac
    67981 tcccatgttg gtgacctccg gctacgactt ctacaccctg cgcagcagca acttccacta
    68041 cagccagcca gacgatttgc gcggccgcct gatcggtgtg tatggcccat ctggcacctc
    68101 attcgtgctg caggaaagcc tgaagaacac tccgaacgtt gaggtcaaaa tggtgaccaa
    68161 taaccatcgc ctattattga tgctcaatgc cggacgtttt ggtgaacaag ccgtagtggt
    68221 gcttaaccgc gatgttgctc gccacttgat cgaacatgaa tatctgtacg cactgcgtca
    68281 cgccggccat ttggcgtcaa tttcatatgg cattgggttt tcccgcaaaa aagtcaccct
    68341 agcgcagttt caggcattca atcgcgggct gagcacactg cgtcataacg gtcagcttgc
    68401 aaccatcctg aaacgctatg gtctacagcc tgccaattaa gcaagctgga catttaactt
    68461 ggcttcgcca taattgcccg gcgcagcaaa atcagccctt taacaaacct gacaattcgc
    68521 tcaggtaaag cacaattaaa gggcacactc gcgcgctaat agtccttgaa actattcgca
    68581 gcgtttataa atgcgcaggc caaaactgct tggccgtctc aggtgctcgg gccatatgcc
    68641 tggcatccag ccaatattct gttcaagacc atgggtaact acgtgacgaa agacgaactg
    68701 cgcgccgaac tcgagcgcca ggcgcagcgt tacaaggatg tatacggcgg ggaagtgatt
    68761 acctacgccg ctcaaccgga ccccgagcgc aagccgtggc gcaagaaggc taaccttctc
    68821 gaccaagcgt ttgaaaaaga gctggtgaaa atcgaaaaag agctgcaaag caaggccgac
    68881 gaggcctctg gctgacgctc gccgccctgc cgccagatca actggccatc cctgagcggg
    68941 atggccactg actcagcgcc ccttgcctcc catcagcgaa cccagcagac cgcgtaccag
    69001 ttgccggccg atctgattgg ccgcctggcg catggctgtt ttcatcacct gactggccag
    69061 gccgccaagc agctcgccag cagcaccggc aatcccgccg gcctcggctt tcttgctcgc
    69121 agcgctcggt ttgctttgct ctgcttgttg cgcggcctgc tcggcgcggg cggtcagcaa
    69181 ttcataggcg gactcacgat cgattggctt gtcgtagcgg ccacgcaacg gcgactgctg
    69241 aatcaaggcc gcacgctcgg cttcactcag cggcccgata cgtgactgcg gcggcgcaac
    69301 ggctacacgc tgcaccatgg ccggcgtgcc tttggcctcc aacgtaccaa ccaaggcttc
    69361 gccgataccc agctcagtaa gtaccgtcaa cgtgtcgata gccggattcg gccggaagcc
    69421 atcggccacc gcacgcagcg acttttgctc tttggcggta aaagcgcgca agccatgctg
    69481 gatacgcagg cccagctgcg ccagcacatc gtccggcaaa tcgcctggcg attgggtgac
    69541 gaaatacacg ccaaccccct tcgagcggat cagtcgcacc acctgctcca ggcgttcttg
    69601 caaggccttg ggagtgtcgg caaacagcag atgcgcttcg tcgaagaaca gcgccagcag
    69661 aggtttgtca gcgtcgccac gttccggcag ctgctcgaat aactcggcca gcagccagag
    69721 caggaaggtt gcataaacct tcggcgcttc atgcaccaaa cggctggcgt cgagcaggtg
    69781 aatgcgccca cgaccatcgc ggtccggatg cagaatgtct tccagctgca gggccggttc
    69841 gccaaacagc gcctcggctc cctgctgttc cagaccggcc aatttgcgca gcaaggcttg
    69901 cgatgaggta ttggtaaaca gcgcgctgtc ttcgccgagc acctcggggt ggcttttcag
    69961 gtgcgccaac agcgccctga gatctttcag gtcaagcagc agcaaacctt cgcgatccgc
    70021 caccttgaat gcggcataaa gtgcggcttg ctgactgtcg gtcagttcca gcagcgcgcc
    70081 gagtagcaaa ggccccatct cactgagcgt ggtacgcagt ggatggccgc tcttgccatg
    70141 cacatcccac agggttagcg ggtaggccat tggctggtga ttgagccagg gcatgctggc
    70201 gatacgctca gccactttgc cttgcggtgc gccggcagca cccagaccgc agagatcacc
    70261 ctttacatca gcggcaaaca ccgccacgcc ggcatcgctg aaggtttcga tcaagcgctg
    70321 cagagtcacc gttttaccag tacccgtagc gccggcaatc agtccgtgac ggttggccag
    70381 acgcaacgcc tgccccaccg gcaagccatc aagaccggcc cccacaacaa actgatcggt
    70441 ttgcatcatc ttgttatctc catggttaaa gctttacttc aactaagccg acatagtcag
    70501 catgccgcat gccctgcggc agccactgcc ttacatcagc acaagactga tgccagaact
    70561 taccggacgc aagaagccat gaccaacaac ctgaagttca gccataagat tctgctcgcc
    70621 gcctcgttgg tggtgatcgc cgccttctcg ctgttcaccc tgtacaacga ttacctgcaa
    70681 cgtaacgcca tccgcgatga tcttgagagt tacctgagcg aaatgggcag tgtgacagcc
    70741 aataacatac agaactggct ctccgggcgc attttgctgg tggaaagtgc cgctcagacg
    70801 atacaaagcg acccctcgcc gtcgcgcgtg atcagcctga tcgagcagaa ggcgctgctc
    70861 tcgacattcg ctttcaccta cctgggcact gccgaaggcg ctttcaccat gcgccccgat
    70921 gccaagatgc cggacggcta tgacccgcgt gcacgcccct ggtacaagga tgcccaggct
    70981 gcaggcggca ccaccctcac tgagccatat atcgacgcag ccaccaacgt accaatcatg
    71041 accatcgcca ccccaatttc accggtcggt gtcgtgggcg gcgatctcaa cctacaagtg
    71101 ctggtcaaga tcatcaactc cctgaatttt gatggtcttg gctacgcctt cctggtcagt
    71161 gctgacggca agatcctggt ccatcccgac gagagtctgg cgatgaagag cctcaaggac
    71221 atctaccctg aaaacacgcc ccccatcagc agtgatttca gtgaggcaga ggtcgacggc
    71281 caaacccgca ttctcacctt cgcgccaatc gagggactgc cgtcggtcaa gtggtacgtc
    71341 ggcctgtcca tcgacaaggc caaggcctat tcgatgctca gcgagttccg cgcctcggca
    71401 atcgtcgcca ccatcatcgc ggtagtgctg atcatgctgt tgctcagcat gctgatcagc
    71461 gtgctgatgc aaccgctgaa tgtaatgggt aaagccatgg aggacatcgc ccaaggcgaa
    71521 ggtgacctga caaagcgcct gagcattgtg tccaacgacg agttcggtcg cctggccagc
    71581 gcattcaacc agttcgtcga acgtatccac agttcgatcc gcgaagtgtc atcggccacc
    71641 agccaggtca atgaagtggc caaattggtg gtcggcgcct ccaactcgtc gatgatcaac
    71701 tccgacgagc aggccagccg taccaacagc gtcgccgcag cgatcaacga actgggcgct
    71761 gctgctcagg agattgcgcg caacgctgcc gacgcttcga atcaggcctc tgatgcgcgt
    71821 catctggccg aagacggccg ccaggtggtg gaaaaaacca tccaggcgat gaatgaactg
    71881 tccggcaaga tcagcgcttc ctgcagcaat atcgaaaccc tcaacagcaa aacggtaaac
    71941 atcgggcaaa ttcttgaggt gatcaaaagt atctcccagc aaactaattt gctcgcactc
    72001 aacgcggcca tcgaagcggc gcgcgctggc gaagccggcc gtggcttcgc cgtggtcgcc
    72061 gatgaagtac gcaacctggc ccaccgcact caagagtcgg cgcaagaaat cgagaagatg
    72121 atcgaggagc tgcaagtcgg ctcacgcgag tcggtcacaa ccatgaccga aagccagcgc
    72181 tacagcgaag agagcgttga gattgccaac caggccggcg cgcgcctgag cagcgtgacc
    72241 gaacgcatcg gcgagattga cggtatgaac cagtcggtgg ccaccgccac cgaggaacag
    72301 acctcggtaa tcgaatcgct caacgtcgac atcaccgaaa tcaacaccct taaccaggaa
    72361 ggcgtggaaa acctcaatgc caccctgcgc gcctgcggtg atctggagca gcaggccagt
    72421 cgactcaagc aactggtcga cagcttccgc atttaacaag tgcgtaaaaa agctgggagc
    72481 catttttaac gtcgcgtagc gacggcccgc aagggtggcc gccatggatg gcaggccata
    72541 caaacaaacg gtgcgctcac tgagcgcccc gtttctattt ctgcttgtcg ctgttcgttg
    72601 caggctgttc gtcctgctgc aattgccgcc ataactcagc ggcatcggta aattcggtgc
    72661 cgtcctcggc ggtcaacgcc tctgggtcat agcgctgcgt acaaccctca ccgagcacag
    72721 gcggcgcgct ggatgttcca cgcgcaagcg gatcagtcat aacacctcca gggcgcgttg
    72781 cggagcttca gctaagcgcc agacgccctt catcatgctg agcgaactcc ttgaccgcac
    72841 gcagcacatc gctgcggctg acctgcccga tcaggcggcc ttcttcgacc accggcaagc
    72901 gccgacgccg cccgcgcaga aaacgctcgg agatttcgat gatatccgcc tcaggcgaaa
    72961 tcacttccac ctcggtggtc atataagcgc tgacatcgcc gcccgccgac tcgtaataag
    73021 cgccagagag gatgccgcgc aagcaatcac cctccgacaa caaacccacc aggtgacctt
    73081 gcccatcgac caccggcgca cccgagatac ggtgttcaag caaacgattg atggcggtga
    73141 acagattcat ttcaggacgg aaagtcacca gattgcgggt catgtagtca cgtactttga
    73201 ttgacttgag catcagtaaa tccctctgtc tgtactgaac cgaaccgcca cctgcgcctc
    73261 agtcgaacac cactgtcttg ttatcgtgca ccagcactcg gtcctctaag tgatagcgta
    73321 gaccacgcga cagcaccatc ttctctacgt ccttacccag acgcaccatc tcttcgatac
    73381 tgtcgcggtg actgacgcgc accacgtcct gctcgatgat cggccccgca tccaactcct
    73441 cggtcacgta gtgcgaggtc gcgccaatca gtttgacccc acgcagcgac gcttggtgat
    73501 acggcttggc cccgacaaaa gacggcagaa agctgtgatg aatattgatc acccgctgag
    73561 aaaattcagc gcagagtggc gcaggcagaa tctgcatata acgcgccaac acgatcacgt
    73621 cagcggcatg ggctttcacc aggcgcgcca cctcatcaaa tgccgactgt ttatcctgcg
    73681 cattgaccgg cacatggaaa tacggaatgt catgccactc gaccatgctg cgcagatcgt
    73741 cgtggttgga aatcacgcag gggatatcgc aatccagttc atcgctgtgc cagcggtgca
    73801 gcaaatcagc caggcagtgc gattcgcgac tggccatcag caccacgcgc ttcttcaccg
    73861 ccgaatcggt cacccgccac tccatggaaa actcacgggc aatcggcgca aaggcctggc
    73921 ggaacccctc cagatcaaat ggcagcgagt cggcgcggat ctcgtgacgc ataaagaacc
    73981 aaccactctg gttatccgag tgatggctcg cttcggtaat ccagccgtta taggtggcca
    74041 gaaagttact gaccttggcc acgatcccaa cccggtccgg acaggcgatg accaatcgaa
    74101 aagtacgcat caacgactcc agcgctcaga ttaaagtcgg ccattctagt cacagccggg
    74161 gaaaactgca gtactcaaac aacccagcca acacagccat ggcgcaaacc cgatgaattg
    74221 cagcaacgcc ctttgagcaa acaccaccgc aataacagat gaataactag gcacgcacaa
    74281 cagataacgc cctcgataag ttacttaaca atacaattca taaaaaatac cgacaaagcg
    74341 gtttacttgt gaaaagtgcc tgactactat tgctatcact aaatcaccaa tcccgaatac
    74401 aggtaccccc acatgtcgtt gatcaacgaa tacagcgcca ccgaagaagc catcaaagaa
    74461 ctgcaagatc gcctgaaaaa tctgtcgcaa gacgacaaac tgaaaaaaga attggaattc
    74521 gaaggcaaac tgcgcgcact gatgggtgaa taccagaagt cgctgcgcga cattattgcc
    74581 atgctcgacc cggacgctaa aggcagcaaa gtcacccgcg cgaccaaaac caccggcacc
    74641 aagcgcgcac gtaaagttaa gcagtacaaa aacccgaaca ccggtgaagt tatcgaaacc
    74701 aaaggcggca accacaaaac tctgaaagag tggaaagcca agtggggcgg tgacgtcgtt
    74761 gaaggctggg caaccctgct gggctaagcc tgtcgcatac gcaacacccg ctttaactaa
    74821 aaacgccagc gctgctggcg ttttttatgg gcgctgtttc agcagagccg ctggcactta
    74881 ccttatggat tacagggcat agcgctggcg caactgttct gcataatcac tccactcaag
    74941 caagatagct tgctgctccg cgccaagatc aggcagtgcc gcggcaagtg cctggctgaa
    75001 ttgttctagc gtgttcggtg caccatattc gggctggctc aaacgctgtt ggcagaatgt
    75061 ccaccaacgc tgttgctctt cagcactcag cgtcgcagca aagttacgcg cacgataacg
    75121 aaacaacagc tccggtaaac gcccatcatc aaatggccaa actgcttcgg ccaaacgctg
    75181 cggctcggca cgccgaacct gttcgcacag tcggcgatca cgatcaccaa taaatccatc
    75241 gtatagctgc tgctcagggt cttgattggc ggcgaactgc tcctcggcat aaacctgggc
    75301 aagtttgtcc tgccacagcc caggattaat acgcaattgt tcggcctgcg cctgataagc
    75361 cggcacatcg agttgcaggc gctgaatatc ttcctcgcgc aatacattca tcggcgcaac
    75421 aaccgggcaa cgattgatat gcaataactt cagcggtacc ggcaactcac cctcggccaa
    75481 ttgatcgcgg cgggtgtaga ggcgcgtgcg caatgtctca gcggattcat tgagcagcgg
    75541 cgctatatca gcctgcagat cacagacgat cagcgcattg cggttgcgcg ggtgccaggc
    75601 cagcggcaat acaacagcca ggtaatgccg cgccccggcg taacgccctg atatatgcac
    75661 cacgggttgt agcagacgta tttggtcctg cacacgctgt ttgctgcgca gttgatagag
    75721 gtagtcatag agtttgcgct gtttgatgcg cagcaggcgc gccaaggcga tggtggcccg
    75781 cacatcggat agcgcgtcgt gggcctgccc gtgctcaatg ccattggccg cagtcaggcg
    75841 ctccagcttg agggtcacgc ggccgtcttc ttccggccag acaatccctt cagggcgcaa
    75901 tgcataggcc gtacgcacca gatcgatcag atcccaacgg ctgttgccgc cctgccattc
    75961 acgggcatag gggtcgaaga agttgcgata caggctgtag cgcgtcactt catcatcaaa
    76021 ccgcaggctg ttgtaaccca ccccacacgt gccgggctgc gacagctcgg catgcacacg
    76081 gctcataaac tcggcttcat ccaggccatg ctgcgccagc gtgcttgggg taatcccggt
    76141 caccaggcag gctgccggat gcggcaggat atcgtcgctg ggctggcagt agatattcag
    76201 cggctccgcg atttcattga ggtcttcatc ggtgcgaatc cccgcgacct gcagcggccg
    76261 gtcgcgccgc gggtcgatgc cggtggtttc gtagtcgtac caaaaaaggc tggaagtcac
    76321 tgtcatgtcc tgatgctgag gcgcaggcag tctaccaccc tgcgagcatc acgcagacaa
    76381 gtgcagaacc ttgcgccagc gcacgtctta cgcggtgcct gcgccttgta gggcgcactg
    76441 gcggcgacta aagtgggggt tctaaaacca ctctgcacaa ggacgcccca tgagtgatat
    76501 cagccccagc aatccctacg cagccccagc cagcgcactg caggaagcgc cgcgcgacag
    76561 ccaggtgccg agcatcgaag aagcgctgag tcgtggctac gacttcaaca tcagtgacct
    76621 gctcagcgaa gcctggcaac gggtaaaagg caccaaaggc atcatcatcg gcggttttct
    76681 ggtgttctac gccgtgctgc tcgcagcgtc cttcctgatt ggcggctttt tcggcctgtt
    76741 gggtgtggtc agcgacagcc caatgctcgc ggtcgtggcc gaactggtaa tcggcattct
    76801 ggcctcggcc ctggcctacc cctttatggc cgggatcaat atgatcggta tccgtcgcgc
    76861 cgccgaccaa ccgatcagct tcaacgacat cttcagccac ttcggccgca ccgtgccgct
    76921 ggtgattacc gccattatca gcatgctgct gatctacctc ggcatgttcc tgctgatcat
    76981 cccaggcctt tacctggcta tcgcctatat gttggccatc ccgctggtgg ttgagcgcgg
    77041 tcttacgcca tggcaagccc tggaagcctc gcgcaaggcg attacgcagc actggttcaa
    77101 ggtttttggc ctgtttctgc tacttggctt gatcacgctc gtcagcgcaa tcccgctggg
    77161 tatcggtctg gtctggacca ttccgttgtt tgtcatcgcc atgggcgtgc tctatcgcac
    77221 cattttcggc gtactgccag caccgcaata aaccctcact aaatagcccg ctgcgccggt
    77281 tcagatcact gagccggttg cttccctgcc tgctgctggc tagcatcggg ctttcctcga
    77341 cgcagtctgc cgatgtcgcc cacgccaacc cctgcttcag tcgccgctcg cccgctgctg
    77401 gatactcgct ataaggtgga aacaccggaa ggcatcgacc tgatcctgcg cccggccggt
    77461 gtagtgccgc gcgccctggc attcagcatc gacctgctga ttcgcggtgc gctgctcggc
    77521 gcattgtttg ccgtgctcgc cctgctcggc cagttcggta tgggcctgag caccatcctg
    77581 ctgtttctcg tgacctggtg gtacatggtg ctgtttgaag tgctcaacca gggccgttca
    77641 cccggcaagc agatgatggg cctgcgagtg attcatgatg acggcacacc gattggttgg
    77701 gccgcctcgc tgacccgcaa cctgctgcgt ttcgtcgaca tcctgccgtt tggctacacc
    77761 ctgggcattc tcagttgcct gaatcacccg gcgttcaagc gcctgggcga tatcgccgcc
    77821 ggcaccctgg tggtgtatcg cgataacgcc ccggccgccc ccgaactacc gcaagctgaa
    77881 gcgctgcgcg caccgtttag catgagtctg aatgaacaac gcgccctgct cagttttgcc
    77941 gaacgcgcgc agagcctatc cagcgcgcga cgccaggagc tggcgagtat tcttgccgaa
    78001 cctttgcagg tgccacccga gcaggccgaa cagcagatcc acggtattgc ccgtggcttg
    78061 ttggggccga catgaaacag agcctgtttg aacagcacca ccaggccgac tggcaagcct
    78121 tcaatgccca gctcgaagcc cttgagcgtg gcaaggccga cagccaacaa tgcaaggcct
    78181 ttgccgccaa ctaccgcagc ctgtgccaac acctggccct ggcgcagagc cgtggctaca
    78241 gcagccacct gatcgaacaa ttgcagctgc tggccatgcg cggccatcag cagttctatc
    78301 gccaccgcag cccattgggc ggccaattac tcggctttat cctcgccggg ttcccgcgct
    78361 tggtccgcgc cgaatggcgc tttgttgccg ccgccagcct gctgttcttc ggcagcctgc
    78421 tgctgatggg cagcctggtg tatcactttc ccgacctggt ctacggcgtg atggcacccg
    78481 accaggttgc aggcatggaa aagatgtatg accccgacgc caggcgcatc ggccgcttta
    78541 gcgaacgcga ctccggcgat gactggatga tgttcggcta ctacatcatg aacaacatcg
    78601 gcatcgcctt tcagaccttt gccagcggcc tgctgtttgg cctgggcagc ctgttcttcc
    78661 tgctgttcaa cggtttgatg atcggcgcag tagccggcca tttgacccag atcggctatg
    78721 gcgaaacctt ctggtcgttc gtagtcggcc atggcgcgtt cgagctgacc gccattgctc
    78781 tggccggtgc cgccggtttg aaactcggct gggcgctgct ggtgcccggc cgcctgccac
    78841 gcggcgaagc cttgcgggta gccgccggca gcagcgtgcg cttggtcggt ggggtcattg
    78901 tgtttctgct gctggcggcc tttatcgagg cctactggtc atccatggac ctagccagcc
    78961 ccttggttaa atactgggta ggcgccggcc tctggctgct ggtactggct tatctgctgc
    79021 tggctggccg aggtgtgcat gcgcctgact gatgccagcg tcgccattcg cccacgcagc
    79081 gcctgggaag ccgtcgatct cggcgtgttg ctggccaaac agcatgccgg cctactgatg
    79141 gccagctggg cgctggtgac cgtgccaatc tttgccctgc tgtgcctgct gctgtgggat
    79201 tacccaagca tcgccctgct gattttctgg tggctgaaac ctgcctatga gcgcctgccg
    79261 ctgtatatcc tctcgcacgc cctgtttggc tccacgccca gcctcaaaca agcgctgaaa
    79321 gccctgcccg gcctgctcaa accacaactg ctggccagcc tgacctggcg ccgactgagc
    79381 cccacacgca gcttcgacct gcccgtgcag cagctcgaag gtctgtccgg caaggcccgc
    79441 agccagcgcc tgattgtcct cggtcagcgt gatgccggcg gcgccagctg gctgacgata
    79501 gtcggcatgc acctggagat ggcgctgtgg ttcggcatca tcagcttcgt ttacatgctg
    79561 ctgccggcgc aaatggagct ggactggacc tgggacagcc tggtcaatgc cgcttctggc
    79621 gaatggctgt ggctggaaca cctgtccaac ctgatctacg tcctgctgct gattctctgg
    79681 gaaccgatct acgtggcctg cggcttcacc ctctacctga accgccgcac cgccctggaa
    79741 gcctgggata tcgaactggt attccgccgc ttgcgccaac gcctgaccgg cgtggcctat
    79801 gccctgctgc tgggctgcgg tgtgctgtta atgcaggcac ctaacagcgc catggccgcc
    79861 gcaccagtaa gcagctgtcc gctgccgatt gaagacccca ttgggccgca agctgaacgc
    79921 ctgctcaaac aaccgctgac cagcctggga gcgcaggaga gcatcagcca gctgctcgat
    79981 cagccgccct tcgaacaccg cgaaaccgtc acccgctggc gcctcggcga cgaacccaag
    80041 gagccccgcg aagaagactc caaggccctg atcgagctgc tggagaagtt cttcaaactc
    80101 acggaaatgt ggaagagcgt cgacgccgtg gcgctgttct tcgaggtcct gctgtgggct
    80161 gctttgttta gcctgattgc ctggctgctg tggcgctacc gcgactggct gagcaccttt
    80221 gccgggcgct tgcgtctgcc acaagggcgc acctatcagc cgccagaaca gctgttcggc
    80281 ctggaagtgg cgccacaaag cctgccagcg gatattcccg gtgaagttga gcggctctgg
    80341 gatgaacagc cacgcgctgc cctcggcctg ctctaccgcg ccctgctcag ccgcatgctg
    80401 catgaacagc gcctgccgtt gaaaagctcg cacaccgaag ccgaagtgct gcaactggtg
    80461 caggccctgc aacaaaaaga cctcagccac ttcagccagg ccctgactcg ccactggcag
    80521 aacctcgctt acggccatcg cctaccgcca gctgcgctca agcagggcct gtgcaatgcc
    80581 tggcggcgac tgttcgaaca aggggtgcag gcatgaaccg gcgcatccag ttccttctcg
    80641 gtgccggcct actgttggtg ctcggcttgc tcgccatcta cctgctgggc aagctcacgc
    80701 cctatcagga aacccttgaa cacggccccg atcccgaagc gcaggccaat ccttacctgg
    80761 ccgccgagca tttcctgcgc aagcagggcc tgcaagtgca acgggccgat ggcctggaag
    80821 tcatgaagga cctgccgagc gcagggcaaa ccctgctgct gctcggcgac cgcaaccgga
    80881 tgacgcccaa gcaggccgaa cgcctgctgg cctgggccgc caagggtggt cacctgctgt
    80941 ttgtcgccga acggctgtgg gatgaagaag aaggcaaaag cggcgacctg ctgctcgaca
    81001 gcctgggcat ccagcaatac gagagcgaag agctcgacga agacgccagc gaagcgacgg
    81061 atgaagaaac ctcaaccgag gacgagacca acgccgagca gcaagccgag cccatgccag
    81121 atgaagaggc acctgccgca gcagacgaag gcaacgccta cccggaactg acccagcttt
    81181 atctggaaaa tgaacaggcg ccggcctact tcaacttcga taccgacttc cacctctacg
    81241 atgccgagaa ccgcgctcac gcctgggcca acagcgacgc ggccacccac ctactgcagc
    81301 tctaccatgg tgatggtttg atcagcgtgg tcagcgacag ctggatctgg caaaccgaga
    81361 gcatcggcga atacgacaac gcctggctgc tctggtacat gacccaggac agtcaggtca
    81421 ccctgctcta tcgcgccgac cgcgataacc tgttcagcct gctgctcgag cacttccctc
    81481 aagcgctggt ctccctggcg ctgttgctgg cattcggcct atggcatttg gggatgcgcc
    81541 aggggccgct gttattaccc gccagccgca accgccggca gttgcaggag catttgcgtg
    81601 gcagtgccga tttcctcctg cgccacaacg gccagcacag cctgctgcaa ggcttgcaac
    81661 aggacattca gcgccgcgcg cggcatcgtc accccggttt cgaacgcctc gctgtggccg
    81721 aacaatggca ggtgcttagc cgcctgaccc gccagccttc cagcgttatc agccaagcca
    81781 tgcggccgtt gcccaagcag cgactggccg ccgccgactt cacccgccag gtcgccaacc
    81841 tgcaaaccct caggaatgcc ctatgagcga gcacacacca gaacaaccgg aaagcgcaat
    81901 cgaacctgct gccgtaacca ctgagcccgt caccgaggcc cctgctgcgg caccagcgcc
    81961 agctgccccg gcagccgctg cggccaatcc aaatgctcag cgtcaacgcg ccagccagtt
    82021 ggctcaagcg ctgcgcgctg aattgcagaa agccctgatt ggccagacgg cggtgattga
    82081 cgatgtgctc agcgcgctga tcgccggcgg ccatgtgctg gtcgaaggcg ttcccggcct
    82141 cggcaaaacc ctgctggtgc gcgcgctggc gcgctgcttt ggcggtgagt tctcgcgcat
    82201 ccagttcact ccagacctga tgccaagcga cgtcaccggt cacgccgtgt acgacctgca
    82261 gagcgagcag ttcaagctgc gcaagggccc ggtattcacc aacctgctgc tggcggatga
    82321 aatcaaccgc gccccggcca agacccaagc cgcgctgctg gaagtaatgc aggaacgcca
    82381 ggtcaccctg gaaggccgcg ccctgaatgt gccgctgccc tttatggttc tggccacaca
    82441 gaacccgatt gagcaggaag gcacctaccc gctgccggaa gctgagctcg accgctttat
    82501 gctcaagctg cgcatagatt atccccagca gagtgaagag ctgaacatgg ttcgccaggt
    82561 ggcgcgctca agcaaagccg acatgctcga agtcaccccg ctgcgcacac tgctgcaggc
    82621 ccgcgacgta ctggcgctgc agaagatcgc cagcgacctg ccgattgacg agcaggtgct
    82681 cgactatgcc gtacgcatcg cccgcgccac ccgcacctgg cctggcctgg ccatcggcgc
    82741 cgggccgcgc gcctcgattg ccctggtacg ttgtggtcgt gcccgtgccc tgctgcgcgg
    82801 cggcgatttc gttctgccgg atgacatcaa gggttgcgcc ctggccgtgt tgcgtcaccg
    82861 cgtgcggctg tcgccggagc tggatatcga aggcttgtca gtcgatcagg tgctgcaaca
    82921 actgctcgac caggttccgg caccgcgcct atgacactca cattgaaaaa gcccgcttcg
    82981 aaaggcgcta cggcatgaaa ccctcgcgcg cgctgctggc gctgctggct ggcctgttcg
    83041 cccttggcat tgccttgggc gtctgcagcg ccctggccat cgccgtgccg cgcagcttta
    83101 ccccaattta ctggggcctg ctgctggtgc tgctcggtct cgcgttggtc gacgccctgc
    83161 tgctgcgcca ggtgccgtcg ccgcgcatcg agcgccagct acccggcaac ctgccgctgg
    83221 ggcgctggag cgaggtgcgg ctgaatctgc accacagtta caaccgcccg cagcaggtgg
    83281 aggtattcga ccatctgcct ggcgatatgg ccttcgaaca cctgccgctc aaggtcgacc
    83341 tgcaccccgg cgaacagacc caggtcagct atcgcgtgcg cccgctgctg cgcgggcact
    83401 ttcactttcc ccgatgcgaa gtgagcctgc ccagccccat gggcctgtgg cgcgcccggc
    83461 ggtatttgcc gctggctgca gaaacccgcg tctacccgga ctttgcccgc ctgtatggcg
    83521 cgcaattgat ggcggtggat gactggctca gccagcttgg cgtgcgccag cagcaacgcc
    83581 gcggtctggg tcaggagttc caccagctac gcgaattccg tgacggcgac accctgcggc
    83641 agatcgactg gaaggccacc gcgcgcaaac gcacaccgat tgcccgcgaa tatcaggacg
    83701 agcgtgacca acagatcatc ttcctgctcg actgcggtcg gcgcatgcgc agccaggatg
    83761 gcgagctgtc gcacttcgac cacgccctca acgcctgcct gctgctcagc tacgtggcgc
    83821 tgcgccaggg tgatgcggtg ggcctgtcga cctttgccgg cgagcagaac cgcttcctcg
    83881 ccccagtcaa aggccaggca cagctgaatg tgctgctcaa tgcgctgtac gacctgcaca
    83941 gcacccagca accggcggac ttcagcgctg cagtggaaaa cctgctcagc cgccaacgtc
    84001 gccgcgcact ggtggtgctg gtaaccaacc tgcgtgatga agatgatcag gcactgctcg
    84061 gcgcagtcag acgcctgagc cgccagcatc gcgtgctggt cgccagcctg cgcgaggaag
    84121 tgctggatac cctacgccac acgccagtgc aggacctctc cagcgctctg gactactgcg
    84181 gcaccctaga ctacctgaat gcccgcgcta gcctgcacga acgcctgatt gccaacaagg
    84241 tgccagtgct cgatgcgcgc ccgagcgagt tggggccgca gctggtgagc agttatctag
    84301 cctggaaaaa agctggcgta ctgtaagggt ccgactagga tctgctgcgc gtcggcgcta
    84361 cagccaggtg acgtgctgtt ccagcggaaa gcgcagacgt gggttgtaca cgcccttctc
    84421 atcctgtttg cccaccgcca acagcatgat cggcaacgcc tgacgcggga tgctcagggt
    84481 cttgcgcagg cgcacttcat caaagccttc catcgggcaa gtggcatagc catggctctg
    84541 gaaggccagc atcaggttct gcgccgccag tgccgtggat ttcaccgccc agatacgcat
    84601 ctgcgcctgg ctgttgggcg cgcgcatcaa tggcttgcgc caggccgcca cacgcagcag
    84661 ctgacgctta aacaaaccaa gcagaccgaa tgggccctgg ttgtactgaa agggtgcggt
    84721 cttgctgtag aaactgcgaa tccgcgccgg cacttccggc tccggccagt agtcgatgat
    84781 gttctggcaa gcctgcttcc aggtgtccgg ccgagccagc acggcaatga tcagcggcgc
    84841 cttggcggcg ttctggctca tgcacaccgg gtgcaaacgg gccagcaacg ccgggtcgcg
    84901 aatcacctga aaactccagg gctgcaggtt gcaggaattg ggcgcgagca ccgccagctc
    84961 caggcaatca cgcaccacct cgtcgggaac cgcttcggcg gtaaaacgac gcaccgcgcg
    85021 gcgacgctcg ataagctcac gtagtgcggc gggggtagcg ggagagatta cggtgtcggc
    85081 gggaaacaga ctcatcaggg catcctcggc aggtcgcccg attaagccgc gatgccgcgt
    85141 gctgaacaag cggctgcggt ggatagtccg cagccttggc gcaaatggcc ttagcccaag
    85201 gccttttcaa tagcctgaat gacgctcgga tcatccggcg tggtgcgtgg cgagaagcgc
    85261 gctaggacgc ggccgtcgtg accgacgagg aatttctcga agttccaggt gatatcacca
    85321 gggaactcgg cgccctcgcc cgccagcaga cggtacagcg ggtgacgctc cggcccgttc
    85381 acctcaagct tgctgcccaa cgggaaggtc accccgtagt tgaggctgca aaactcgcga
    85441 atggcttcct cgctgtcagg ctcctgctcg gcaaactggt tgcacggcaa acccagcaca
    85501 ctgaagccct gactcttgta ctgctgatag agcttttcca ggccggcgta ctgcggcgtc
    85561 aggccgcact tggaggccac atttaccacc aacaccacct gccccttgaa gggtgccagc
    85621 ggcagctctt ggccatccag tgcacgcagg ttgaggtcgt gaaaggcact catggctaac
    85681 tccttgagaa cacacaggtt acgtgtagcg gacaaaaaag gcgcctatct gaaggcgcct
    85741 ttcttctgtt gcagcttagc agtcgagcca gcggttgata cgaccataag tcgactcgcg
    85801 gctatgacat aaggctacac cttagaatct gtttcggatc tcgcgagcta gagtcctgca
    85861 aggcaaaaac aggcgaggaa gcggagtgta ctgttgtaca tgagcattcc gagcctgttt
    85921 ttaacgccgc agggccgacg cgcagcagat ccgagacggg ttcttagtga tggtgaccac
    85981 cttcgccatg aatatgccca tgagcgactt cttcttcgct ggcatcacgc acgctgatga
    86041 ccttgacctt gaagtgcagg cgctggccag ccagtgggtg gttgccgtca acgatcacgt
    86101 cgtcgccttc cagttcgcgg atggtaacga tctgcatgcc gccgtccggg ccggaggcgt
    86161 ggaactgcat gccgacttcc agctcatcaa caccttcgaa catcgaacgg ttgagggtgg
    86221 ctaccagctc ggcgctgtat tcgccgtaag cgtcttccgg ctcaactgca acttccagct
    86281 cgtcgccggc ttgcttgccc agcagggctt tttccaggcc gccgatgatg ttgccggcac
    86341 cgtgcagata agccagcggc gcgccgcctg cggaactgtc gatcacctca ccggcatcgt
    86401 tagtcagggt atagtcgatg gagacggcct tattggcggc gatcagcatg gggcgagacc
    86461 tt
//
LOCUS       DASRUM010000024         7557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1518637, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000024 DASRUM010000000
VERSION     DASRUM010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7557
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518637"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..68
                     /locus_tag="VFF22_01990"
     CDS             <1..68
                     /locus_tag="VFF22_01990"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252496.1"
                     /note="catalyzes the formation of nucleoside triphosphate
                     from ATP and nucleoside diphosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZX15625.1"
                     /translation="ASAAREIAYFFAATEVCARIR"
     gene            92..1240
                     /gene="rlmN"
                     /locus_tag="VFF22_01995"
     CDS             92..1240
                     /gene="rlmN"
                     /locus_tag="VFF22_01995"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007247079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZX15626.1"
                     /translation="MTEATGKINLLGLTQAEMEQFFERIGEKRFRAGQMMKWIHHFGI
                     DDFDAMSNVSKVLREKLKACAEIRGPEVVSEDISTDGTRKWVVRVASGSCVETVYIPQ
                     GTRGTLCVSSQAGCALDCSFCSTGKQGFNSDLSAAEVIGQVWIANKSFGSVPAKIDRA
                     ITNVVMMGMGEPLLNFDNVVAAMKIMMDDLGYGISKRKVTLSTSGVVPMIDKLGEVID
                     VSLALSLHAPNDALRNQLVPINKKYPLEMLLAACKRYVSRLGEKRVLTIEYTLLKDVN
                     DKTEHAEEMIALLKDVPCKINLIPFNPFPHSGYERPSNNAIRRFQDLLHKAGHNVTVR
                     TTRGEDIDAACGQLVGQVMDRTRRSERYIAVRELSSEAETPSSAANRT"
     gene            1254..2012
                     /gene="pilW"
                     /locus_tag="VFF22_02000"
     CDS             1254..2012
                     /gene="pilW"
                     /locus_tag="VFF22_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003227892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HZX15627.1"
                     /translation="MTVRPALLLLIVGLLAGCVSTGNVDPMKTDKGRNEARDAYTQLG
                     IGYLQNGASERAKVPLKKALELDPSSADAHAALALVFQMEMEPKLADEHFRKALSQRP
                     DDARLLNNYGSFLFEQKRYQEAFERYTQAAQDTLYPERSRVFENLGLTSLQLKQREQA
                     KAYFERSLRLNSRRPRALMEMAQLSYEDKQYVPARGYYESYTALAQPDARSLLLGVRL
                     AKIFEERDNAASLGLQLKRLYPGTPEYQQYLSEQ"
     gene            2012..3010
                     /locus_tag="VFF22_02005"
     CDS             2012..3010
                     /locus_tag="VFF22_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014336697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ family helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZX15628.1"
                     /translation="MKAAHPEVLAVPRINPGETLRKARESRGWTIAEVAAQLNLTPQR
                     LSQVEQGAFDKLPGHTFARGYVRAYAKLLEVDQNRLVLEFDQFTGSDAAGSSVHSLGR
                     IEEPVSYSQRILRMVSFVLLLALAGLSFFWWQEQAERRAEDLASTSLEHVEVDGADGT
                     TQIHPLDEPEDQAVDAAQLDTEAMLLSLSEGAAELPGAVGSEPAAVGPQAEQPQALGE
                     TASAPAVSAAPAEAAPVIAGAVPASPAPVQAAAGEAVVSLTFIADCWTQLTDANGKVL
                     FSALKRKGDSLQLAGKAPLELRLGYARGVQISLNGNPVDVAPFISGETARLKLGGQ"
     gene            3012..4121
                     /gene="ispG"
                     /locus_tag="VFF22_02010"
                     /gene_synonym="gcpE"
     CDS             3012..4121
                     /gene="ispG"
                     /locus_tag="VFF22_02010"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZX15629.1"
                     /translation="MHCESPIKRRISRKIWVGSVPVGGDAPIAVQSMTNSDTNDVAAT
                     VAQIRRLEDAGADIVRVSVPDMDAAEAFGKIKQQVKVPLVADIHFDYQIALRVAELGV
                     DCLRINPGNIGREDRVRAVVEAARDKGIPIRIGVNAGSLEKDLQKKYGEPTPAALVES
                     ALRHVEHLDRLNFPDFKVSVKASDVFMAVEAYRLLAKQIEQPLHLGITEAGGLRSGTV
                     KSAVGLGMLLAEGIGDTIRISLAADPVEEIKVGYDILKSLRLRSRGINFIACPSCSRQ
                     NFDVVKTMNELEQRVEDLLVPLDVAVIGCVVNGPGEAKEAHIGLTGGSPNLIYIDGKP
                     ALKLGNDNLVDELEKLIRQKAADKVAADAALILRS"
     gene            4140..5429
                     /gene="hisS"
                     /locus_tag="VFF22_02015"
     CDS             4140..5429
                     /gene="hisS"
                     /locus_tag="VFF22_02015"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZX15630.1"
                     /translation="MSKSLQAIRGMNDILPEQTPLWRYFEGAVASLLDGYGYRQIRMP
                     IVEFTELFKRSIGEVTDIVEKEMYTFDDRNGDSLTLRPEGTAACVRAVLEHGLAGGGQ
                     TQKLWYIGPMFRHERPQKGRYRQFHQIGVEVFNIDGPDIDAELIVLTWRLWGLLGIRD
                     AVKLELNSLGTSAARAVYRDALVEFLTAHADQLDEDSRRRMGSNPLRVLDSKVPETQA
                     LLVNAPKLADYLDEESRVHFEGLKARLDAAGVPYAINPKLVRGLDYYSKTVFEWVTDQ
                     LGSQGTVCAGGRYDGLVEQMGGKPTPGVGFAMGIERLVLLLETLGKVPAEIARTVDVY
                     LCAFGEPAELAALTLSERLRDQLPNLRLQVNAGAGSFKSQFKKADKSGALYALILGED
                     ELAQQVVGCKPLRGQGEQQSIAWSALADHLATCADQA"
     gene            5530..6111
                     /locus_tag="VFF22_02020"
     CDS             5530..6111
                     /locus_tag="VFF22_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZX15631.1"
                     /translation="MPLLTGVALALVVVFGWQGWQKYQANQSQGASLVYQQLLEAALD
                     PSGKPDASKVAELANTLKNDFSGTHYAQYGSLFVAKVAVEAGKLDDAASELQAIVDKP
                     ADDSLAELARQRLARVLAAQDKAEDALKLLDAKVAPAFAASREELKGDLLVQLGRTDD
                     AHAAYEKAKTALSEDAAVGGLQMKLDDLAKGDA"
     gene            6104..7255
                     /gene="bamB"
                     /locus_tag="VFF22_02025"
     CDS             6104..7255
                     /gene="bamB"
                     /locus_tag="VFF22_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011063204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HZX15632.1"
                     /translation="MRDVMRWKNAALLALAVLAVGCSSNSKKELPPADLPKFEEEVSL
                     QKEWSRSIGDGQGESFNMLVPAIDGEQIYAADVEGLVVAMDRMTGKVIWKADLELPVS
                     GAVAAGYGLVVVGTLKGEIIALDTANGEEKWRARVTSEVLAPPALNGDVVLVQTQDDR
                     LIALDMNTGEQRWIFENTPAVLTLRGTGSPVLTNRLAIAGLSTGKVIALDAQRGIPVW
                     EQRVAVPQGRSELDRVVDIDGGLLLSAGTLYAVSYQGRVAALDLESGRILWQREASSS
                     VGVAQGYGSVYVSLASGTVEGIDERSASALWSNDALARRQLSAPEVFSSYIAFGDLEG
                     YLHLVSQVDGRFVGRTRIDSDGLRARPLVVGDWLYAYGNGGNLVALTIK"
     gene            7384..>7557
                     /locus_tag="VFF22_02030"
     CDS             7384..>7557
                     /locus_tag="VFF22_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZX15633.1"
                     /translation="MVPVIALVGRPNVGKSTLFNRLTKSRDAIVGDLSGLTRDRQYGE
                     AKWQGRTYIVIDTG"
BASE COUNT         1524 a         2061 c         2380 g         1592 t
ORIGIN      
        1 aagcctctgc tgcccgcgaa atcgcttact tcttcgctgc caccgaagtt tgcgcacgta
       61 ttcgctaagc gaatcgggtg agggtgaagc catgactgaa gctaccggta aaatcaatct
      121 gctgggtctg acccaggcag aaatggaaca attcttcgaa cgcatcgggg aaaagcgttt
      181 ccgtgccggt cagatgatga aatggattca ccactttggc atcgatgatt tcgatgccat
      241 gagtaacgtc agcaaggtct tgcgcgaaaa gctcaaggcc tgtgctgaaa tccgcggccc
      301 ggaagtggtc agcgaagata tttctactga tggcacgcgc aagtgggtag tgcgagtggc
      361 gtcaggcagt tgcgtagaaa ccgtgtatat cccccagggc acgcgcggca ccttgtgtgt
      421 gtcgtcgcag gccggctgtg cgctggattg cagtttctgc tcgactggca agcaaggttt
      481 caacagcgat ctgagtgccg ccgaagtgat cggtcaggtg tggatcgcca acaagtcgtt
      541 cggcagcgtg ccggcgaaga ttgaccgtgc catcaccaac gtggtgatga tgggcatggg
      601 tgagccgctg ctgaatttcg acaacgtcgt cgccgccatg aaaatcatga tggatgacct
      661 cggttacggt atctccaaac gcaaggtgac gctgtccacc tccggcgtgg tgccgatgat
      721 cgacaagctc ggcgaagtga tcgatgtatc cctggctttg tcgttgcatg cgccgaacga
      781 tgcgctgcgc aaccagttgg taccgatcaa caagaagtac ccgctggaaa tgctactggc
      841 ggcgtgtaaa cgttatgtct cgcgccttgg cgagaagcgc gtgcttacaa tcgaatacac
      901 cctgctcaag gatgtaaacg ataagaccga gcacgctgag gagatgatcg cacttctcaa
      961 agatgtgcct tgcaagatca acctgattcc gtttaatccc ttcccccact ccgggtacga
     1021 gcggccgagc aacaatgcaa ttcgccgttt ccaggacctg ctgcacaaag ccgggcacaa
     1081 tgtcaccgta cgcaccactc gcggtgaaga catagacgcg gcttgtggcc agttggtcgg
     1141 tcaggttatg gaccgtaccc gccgtagtga gcgctatatt gctgtgcgtg aattgagtag
     1201 cgaggccgaa acgccgagtt ccgccgccaa tcgaacctga ggggaggaaa tccatgaccg
     1261 tgcgtcctgc gctattgttg ttaatcgtcg gccttttggc tggctgtgtg tcgacgggca
     1321 atgtcgatcc gatgaaaact gacaaaggcc gcaacgaggc acgtgatgcc tacacccagc
     1381 tgggcatagg ctatctgcag aacggggcct cggagcgcgc caaggtaccg ctgaaaaagg
     1441 ctctggagct tgatccctcc agtgccgatg cgcatgccgc cctggcgttg gtgttccaga
     1501 tggaaatgga gcccaaactg gcggacgaac actttcgcaa ggcgctgtca cagcggcctg
     1561 atgatgctcg cttgttgaac aattacggca gtttcctgtt cgagcagaaa cgctatcagg
     1621 aggcatttga gcgttacacc caggcggctc aggacactct ttacccagaa cgctcacggg
     1681 tgtttgaaaa cctcgggctt acctcgttgc agcttaagca acgtgagcag gccaaggcct
     1741 atttcgagcg ttcgctgcgg ttgaatagcc gccggcctcg cgccctgatg gaaatggcgc
     1801 agctgtccta tgaggacaag caatatgtcc ccgcccgtgg ctattacgaa agctatacgg
     1861 cgcttgccca gccggatgcc cgcagccttc tgctgggcgt gcgcttggcg aagattttcg
     1921 aggagcgcga caatgccgcg agtctcggtt tgcagttgaa gcggctttat ccgggcacgc
     1981 cggaatatca gcaatacctg tcggagcaat gatgaaagcg gcgcatccag aagtgctagc
     2041 agttccccgt atcaatccgg gtgagacttt gcgcaaggct cgtgagagcc gcggttggac
     2101 tattgccgag gttgctgcgc agctgaactt gactccgcag cgcttgagtc aggtcgaaca
     2161 aggtgccttc gacaagctac ccggccatac ctttgcgcgt ggttatgtgc gtgcttacgc
     2221 caagttgctg gaagttgatc agaaccgtct agtgctggag ttcgatcagt tcaccggcag
     2281 cgatgccgcg ggcagcagtg tgcatagtct ggggcgtatc gaggaaccgg tgagctactc
     2341 acagcgtatt ctgcgtatgg tcagttttgt tctgttactg gcgctcgctg gtttgagctt
     2401 cttctggtgg caagagcaag ccgagcgtcg cgccgaagac ctggcttcaa ccagccttga
     2461 acatgttgaa gtagacggtg ctgatggcac cacgcaaatc catccgctgg atgaaccgga
     2521 agaccaggcg gtagacgctg cccagctgga tacagaagcc atgttgctgt cattgtcaga
     2581 gggtgccgct gaactgccag gcgctgtcgg cagcgaacca gcagccgtgg gcccacaagc
     2641 ggaacagccg caagctctcg gcgaaacggc aagtgctcca gcagtgtccg ctgctcctgc
     2701 cgaggcggcg cctgttattg ctggcgctgt gcccgcgtca cctgctcctg tgcaagctgc
     2761 cgcaggcgag gcagtggtca gcctgacctt tattgccgat tgctggacgc aactgaccga
     2821 tgccaatggc aaggtgttgt tcagcgcact caaacgtaaa ggcgatagcc tgcagttggc
     2881 cggcaaggct ccgctggaac tgcgtctggg ttatgcccgt ggtgtgcaga tcagcctgaa
     2941 tggcaatccg gtcgatgtgg cgccctttat ctccggtgag accgcgcgtc tgaagttggg
     3001 tgggcagtaa catgcattgc gagtcaccaa tcaaacgccg tatttcacgc aaaatctggg
     3061 tcggttctgt gccggtgggt ggcgatgcgc ccatcgcagt acagagcatg accaatagcg
     3121 ataccaatga tgtagccgct actgtggcgc aaatccgtcg cctggaagat gccggcgcag
     3181 acatcgtgcg tgtctcggtg ccggatatgg atgccgccga ggccttcggc aagatcaagc
     3241 agcaggtcaa agtgccgctg gtggccgata tccacttcga ttatcagatc gccttgcgcg
     3301 tggctgaact cggcgtggac tgtctgcgta tcaatccggg caatatcggc cgtgaagacc
     3361 gcgtgcgcgc ggtggtcgag gcggcgcgcg acaaaggcat tccgattcgc atcggggtaa
     3421 acgccggttc gctggaaaaa gatctgcaga agaaatacgg cgagccgact ccggccgcgt
     3481 tggtcgaatc tgcgctgcgt catgtcgagc acctggatcg cctgaacttc cctgacttca
     3541 aggtcagcgt aaaagcctcc gacgtgttta tggccgtcga agcctatcgc ctgctggcca
     3601 aacagatcga gcagccgctg cacttgggta tcaccgaagc cggcggcctg cgctcgggca
     3661 cggtcaagtc cgccgtgggc ctgggcatgc tgctggctga aggcataggc gacaccatcc
     3721 gcatctccct ggctgccgat ccggtggaag agatcaaggt tggctacgac atcctcaagt
     3781 cgctgcgcct gcgttcccgt ggcatcaatt tcatcgcctg tccgagctgc tcgcggcaga
     3841 acttcgatgt tgtgaaaacc atgaacgagc tggaacagcg cgtcgaagac ctgctggtgc
     3901 cgctggatgt ggcggtgatc ggttgtgtgg tcaatggccc tggcgaagcc aaggaagccc
     3961 atatcggcct gaccggtggc tcgcccaacc tgatctatat cgatggcaag ccggcgctca
     4021 aactgggcaa tgacaacctg gtcgatgagc tggaaaagct gatccgccag aaagcggccg
     4081 acaaggtcgc agctgacgcc gctctgattc tgcgcagcta acaccgttaa ggaattgaat
     4141 tgagcaagtc cctgcaagcc attcgtggca tgaacgatat cctgcccgag cagactccgc
     4201 tttggcgtta cttcgaaggc gccgtggcca gtctgctgga tggctacggc tatcggcaga
     4261 tccgtatgcc tatcgtcgag ttcaccgagc tgttcaagcg ctcgattggt gaagttaccg
     4321 atatcgttga aaaagagatg tacaccttcg atgaccgcaa cggcgactcc ctgaccctgc
     4381 gtccggaagg taccgccgct tgcgtgcgtg ccgtgcttga gcatggtttg gcgggcggtg
     4441 ggcagaccca gaagctctgg tacatcggcc cgatgttccg tcacgagcgt ccgcagaaag
     4501 gccgttatcg tcagtttcat cagattggtg tggaagtctt caatatcgac ggcccggata
     4561 ttgatgccga gttgatcgtg ctgacctggc gcctgtgggg cctgctgggt atccgtgatg
     4621 cggtgaaact tgaactcaat agcctcggca ccagcgccgc gcgtgctgtt taccgtgatg
     4681 cactggtcga gttcctgact gcccatgccg atcagctgga tgaagacagt cgtcgccgta
     4741 tgggcagcaa cccgctgcgg gtgctcgaca gcaaggtgcc ggaaacccag gctttgctgg
     4801 tcaatgcacc gaagttggca gattacctgg acgaagagtc gcgcgtgcat ttcgaggggc
     4861 tcaaggcccg tctggacgcc gccggtgtgc cttacgcaat caatcccaag ctggtgcgtg
     4921 gcctggatta ctacagcaag accgtgttcg agtgggtaac cgaccagctc ggctcccagg
     4981 gcacggtttg cgctggtggc cgttacgacg gcctggtcga gcagatgggc ggcaagccga
     5041 cgcctggcgt gggttttgcc atgggcatcg aacgtttggt gctgttgctc gaaactctag
     5101 gtaaagtacc ggctgaaatc gcccgtacgg tcgatgttta cctgtgcgct ttcggtgagc
     5161 ctgccgaact ggcggcgctg accttgagcg agcgactgcg tgatcagctg ccgaacctgc
     5221 gtctgcaggt aaatgccggg gctggtagct ttaagagtca gttcaagaag gccgataaga
     5281 gtggcgcgct gtacgccttg atcttgggtg aagacgaatt ggcccagcaa gtggtaggtt
     5341 gcaagccgct gcgcggtcag ggcgagcaac aaagtattgc gtggtcggca ttggccgacc
     5401 atctggcgac ctgcgccgat caggcctgag cgcctgttaa gtgatttagc gaataggagt
     5461 gttggggtgg tttcgcgtac tgatgatgaa gaactggcgg taatcaagga ctggtggcag
     5521 cgcaatggca tgccattact gaccggtgtt gcgctggcgt tggtggtggt gtttggctgg
     5581 cagggctggc agaagtacca ggctaatcag tcccagggtg cttcgctggt gtatcagcaa
     5641 ttgctggaag ctgcgctgga tccaagcggc aaacctgatg cgagcaaagt cgccgagctg
     5701 gccaacacgc tgaagaatga tttctccggt acccattacg cccaatacgg cagcctgttt
     5761 gtagccaaag ttgcggtcga agcgggcaag ctggatgacg ctgccagcga gctgcaggcg
     5821 attgtcgaca agccggctga tgacagcttg gctgagttgg ctcgtcagcg cctggcgcgc
     5881 gtgctggctg ctcaggacaa ggctgaagat gccctgaaac tgctggatgc caaggttgcg
     5941 ccggcgtttg ccgccagccg tgaagaactc aaaggtgacc tgctggtgca actcggccgt
     6001 accgacgatg cgcacgccgc ctacgaaaaa gccaagaccg cgctctctga agatgccgca
     6061 gtcggtggtt tgcaaatgaa gctggatgat ctggcaaaag gggatgcgtg acgtgatgcg
     6121 ctggaagaat gccgcactgc tggccctggc cgttctggcc gtaggttgca gcagcaatag
     6181 caagaaggaa ctgcctccgg ccgatctgcc caagtttgag gaagaggtta gcctgcagaa
     6241 agaatggagt cgttcgattg gcgacggtca gggcgagtcc ttcaacatgc tggtaccggc
     6301 gattgatggt gagcagattt atgccgccga cgtcgaaggc ctggttgtgg ccatggaccg
     6361 catgaccggc aaggtcatct ggaaagctga cctggaactg ccggtttccg gcgcagtcgc
     6421 agcaggctat ggcctggtgg tggtgggcac gctgaaaggc gagatcatcg cgctcgacac
     6481 cgctaacggt gaagagaagt ggcgcgcccg tgtgaccagt gaagtgctgg ctccacctgc
     6541 gctgaacggc gacgtggtac tggtacagac tcaggatgac cgcctgatcg ccctggacat
     6601 gaacaccggc gagcagcgtt ggatctttga aaataccccg gcggtactta ccctgcgcgg
     6661 cactggcagc ccggtactga ccaaccgtct ggcgattgct ggcctttcca ccggcaaggt
     6721 gattgcgctg gatgctcagc gcggtattcc ggtctgggaa cagcgtgtgg ctgttccgca
     6781 gggccgttcc gagctggatc gtgtggtcga tatcgacggc ggtctgctgc tctcggccgg
     6841 caccctctac gccgtaagct accagggccg tgttgccgcg ctggatctgg aaagcggtcg
     6901 tatcctctgg cagcgtgaag cgtccagctc ggtcggtgtg gcccagggtt atggcagtgt
     6961 ttacgtaagc ctggccagcg gtaccgttga aggtatcgac gagcgttcgg cttcggcctt
     7021 gtggagcaac gatgccctgg ctcgccgcca gttgtcggct ccggaagtgt tctccagcta
     7081 tatcgcgttc ggtgacctgg aaggttacct gcacctggtc agccaggtgg acggtcgttt
     7141 tgtcggtcgt acgcggatcg acagcgacgg cctgcgtgcc cgtccgctgg tcgtgggtga
     7201 ttggctgtat gcctatggca acggcggcaa tctggtggcg ctaaccatca agtgatcgct
     7261 cgctgacgct ttggctatgc tgcacagcac ctagggctgt gcagcatagc cagtgcaatg
     7321 aattccggcc gctgcctctg cagcggcttt tgtattttct gaaataacga agtggagagc
     7381 ctaatggttc ccgtaattgc cctggtgggc cgccctaacg tcggcaagtc caccctgttt
     7441 aaccgcttga ccaagtcccg tgacgcgatt gtcggcgacc tgtctggtct gacccgtgat
     7501 cgccagtacg gcgaggccaa gtggcagggg cgaacctata tcgtcatcga caccggt
//
LOCUS       DASRUM010000025         7065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_12772, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000025 DASRUM010000000
VERSION     DASRUM010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12772"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFF22_02035"
     CDS             complement(<1..194)
                     /locus_tag="VFF22_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361159.1"
                     /note="The beta subunit catalyzes the decarboxylation of
                     the malonyl moiety on coenzyme A; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HZX15634.1"
                     /translation="MHDANIQRLLAQRSFTELGARQRARALLDPGSFRELIDPFAKLV
                     SPWLPQQGIVPQADDGVVVG"
     gene            complement(187..486)
                     /locus_tag="VFF22_02040"
     CDS             complement(187..486)
                     /locus_tag="VFF22_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017476503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HZX15635.1"
                     /translation="METLTFEFPAGQPSQSRALVGCVGSGDLEVMFEPGRAGTLTIEV
                     VTSVNGSASRWQQLFERMFREQQLPALNISIHDFGATPGVVRLRLEQGLEELSHA"
     gene            complement(489..1367)
                     /locus_tag="VFF22_02045"
     CDS             complement(489..1367)
                     /locus_tag="VFF22_02045"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HZX15636.1"
                     /translation="MSAVNARQQPLSLSECLADLAVDALIDEADLSPKPGLVDRRGSG
                     AHTDLHLGLMHASALSLWPCFKAMADAALERGTINQGLREDIGQLGRDGEVEMLRVTN
                     GVNTHRGAIWALGLLTAAAALDSTALQPGEVSLRAARLAMLNDRRAPANGDSHGAQVC
                     RLYGVRGAREEAQLGFPAVTQQALPQLQRSRAAAAGEQNARLDALLAIMSTLTDTCVL
                     YRAGLAGLSSMQSGARAVLKAGGCASLEGRRRLRELDDDMLRLRASPGGAADLLAASL
                     FLDRLQPALQTQIGSV"
     gene            complement(1367..3028)
                     /gene="mdcA"
                     /locus_tag="VFF22_02050"
     CDS             complement(1367..3028)
                     /gene="mdcA"
                     /locus_tag="VFF22_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HZX15637.1"
                     /translation="MTTTISPPPQWSRRRAEKARRLDQVRTLADGVVLPSDKIVAALE
                     ALIAPGDRVVLEGNNQKQADFLSRSLAQVDPGRLHDLHMIMPSVSRAEHLDLFERGIA
                     RKLDFSFAGPQSLRISQLLEDGLLEVGAIHTYIELYSRLLVDLIPNVALVAGFQADRH
                     GNIYTGPSTEDTPALVEPAAFSDGIVIFQVNEIVDELPRVDIPASWVDFVVVADKPFY
                     IEPLFTRDPRHIKPVHVLMAMMAIRGIYEKHKVQSLNHGIGFNTAAIELILPTYGESL
                     GLKGKICRNWTLNPHPTLIPAIETGWVESVHCFGTELGMENYIAQRPDVFFTGRDGSM
                     RSNRMMCQLAGQYAVDLFIGATLQVDGDGHSSTVTRGRLAGFGGAPNMGHDPRGRRHP
                     TPAWLDMRPSESEAPLLERGKKLVVQMVETFQEGGKPTFVDQLDAVEVAKKSGMPLAP
                     IMIYGDDVTHLLTEEGIAYLYKARSLEERQAMIAAVAGVTAIGLRHNPKDTERMRREG
                     LIALPEDLGIRRADASRELLAAKSIAELVDWSGGLYNPPAKFRSW"
     gene            3224..4762
                     /locus_tag="VFF22_02055"
     CDS             3224..4762
                     /locus_tag="VFF22_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017906203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HZX15638.1"
                     /translation="MRIHVTFIDRVGITQEVLALLGGRNLNLDAVEMVPPNVYIDAPT
                     LSPEVLDELREALFKVRGVQAVTIVDILPGQRRHLQLDALLAAMTDPVLAVDSQGRVL
                     LANPALIELCGQQPEGLGIGELFADSQLQTELLEQKFRLPMREVTLKGQALLLDAQPI
                     TESVDGGARHLAGGLLSLYAPSRIGERLAALHHDHAEGFDSLLGDSAPIRNLKTRAQR
                     VATLDAPLLIQGETGTGKELVARACHAISTRRTAPFLALNCAALPENLAESELFGYAP
                     GAFTGAQRGGKPGLLELANQGTVFLDEIGEMSPYLQAKLLRFLSDGSFRRVGGQSEVK
                     VNVRILSATHRDLEKMVNEGAFREDLFYRLNVLNLEVPPLRERGQDILLLARHFMQQA
                     CAQIQRLPCRLTPDTYPALLGNRWPGNVRQLQNVIFRAAAICESNLVQIDDLDIAGTA
                     VAAQQADGEVSSLEAAVDQFEKALLEKLYPSHPSTRQLATRLHTSHSAIAMRLRKYGI
                     PGKP"
     gene            complement(4772..5539)
                     /locus_tag="VFF22_02060"
     CDS             complement(4772..5539)
                     /locus_tag="VFF22_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZX15639.1"
                     /translation="MSVTIEKNGPVTTLIINRAEVRNAVDRPTAEALAAALRAFEADE
                     QARVAVLAGAGGTFCAGADLGAVVEGAERANRLETEGDGPMGPSRMQLSKPLIAAIEG
                     HAVAGGLELALLADLRVMANNAVLGVFCRRFGVPLIDGGTVRLPRIIGQGRALDLILT
                     GRPVLAEEALSMGLVNRVVAAGSALAAAQQLALEIAGFPQRCMLADRVSAYAQWDLPF
                     VQALGNEFAGGMAVIASGETQAGAQRFKDGTGRHGTF"
     gene            complement(5722..6633)
                     /locus_tag="VFF22_02065"
     CDS             complement(5722..6633)
                     /locus_tag="VFF22_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX15640.1"
                     /translation="MSDAIHFQDQVVIVTGAGGGLGRAHALLFAKHGAKVVVNDLGGS
                     THGEGANASAADKVVEEIRSAGGTAVANHDSVTDGEKIVQCALDTFGRIDVVVNNAGI
                     LRDKTFHKMEDADWDLVYKVHVEGAYKVTRAAWPHMREQGYGRIIFTASTSGIYGNFG
                     QSNYGMAKLGLYGLTRTLAIEGRKNNILVNAIAPTGGTRMTEGLIPPQVFEQLKPELV
                     SPLVVYLASSACEETSGLFEVGGGWMGKVRWERSLGAGFDPREGYSPEDVAANFAQIC
                     DFEGAAHPKDNIEAMKEMMANLQKFAL"
     gene            complement(6766..>7065)
                     /locus_tag="VFF22_02070"
     CDS             complement(6766..>7065)
                     /locus_tag="VFF22_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15641.1"
                     /translation="AAPSGPTDPTGPTSPVPPSIPPVETDTDPDMDNGRDPGLSNDPQ
                     APRPGTDGTSTGQGTGTGTGNGTGNDMDTTPRNNGGATNNDDAGTDTGTGGYGEQ"
BASE COUNT         1293 a         2304 c         2200 g         1268 t
ORIGIN      
        1 ttgccgacaa ccacaccgtc gtcggcctgg ggcacgatgc cctgctgggg cagccagggt
       61 gacaccagct tggcaaaggg atcgatcagc tcgcggaaac tgcccggatc gagcaaggcg
      121 cgggcacgct ggcgggcacc aagctcggta aaactgcgct gcgcaagcag gcgctggata
      181 ttggcatcat gcatgggaca actcctccag gccttgctcc agacgcaggc gaaccacgcc
      241 aggggttgcg ccaaagtcat gaatggaaat gttcagggct ggcagttgct gctcgcggaa
      301 catgcgctcg aacagttgct gccaacggct tgcgctgccg ttgaccgagg tcaccacctc
      361 aatcgtcagg gtgccggcgc gaccgggctc gaacatcact tccaggtcgc cggagccaac
      421 acagccgacc agggcacggc tctggctcgg ttgcccagcg ggaaattcaa aggtcagggt
      481 ttccatgctt aaacactccc aatctgcgtc tgcagcgcag gctgcaggcg gtcgaggaat
      541 aaagacgctg cgagcaaatc ggctgcaccg ccgggcgagg cacgcagcct caacatgtcg
      601 tcatcgagtt cacgcaggcg tctgcgccct tcgaggctgg cgcagccgcc ggccttgagc
      661 acggcacggg ccccgctctg catgctgctc aggccggcca ggccggcgcg atagagcaca
      721 caggtatcgg tcaaggtact catgatcgcg agcaaggcat ccagccgggc gttctgctcg
      781 ccggccgccg ccgcgcggct gcgttgcaac tgcggcaggg cctgttgggt gacggccgga
      841 aagcccagct gcgcttcctc acgggcaccg cgcacgccat acagccgaca gacctgagca
      901 ccatggctgt cgccattggc cggagcgcgg cggtcgttga gcatggccag ccgcgcggca
      961 cgcaggctca cttcgcccgg ctgtaaggcc gtgctatcca gcgcagcggc ggccgtcagc
     1021 aggcccagag cccagatcgc gccccggtgg gtattcaccc cgttggtaac gcgcagcatc
     1081 tccacttcgc catcgcggcc gagctgaccg atgtcctcgc gtaacccctg gttgatcgtc
     1141 ccgcgttcca gtgcggcatc agccatggcc ttgaaacatg gccatagcga cagcgccgag
     1201 gcgtgcatca agcccaggtg caggtcggta tgcgcaccgc tgccacggcg gtcaaccagg
     1261 cccggcttgg gcgacaggtc ggcctcgtcg atcagcgcat ctaccgccag atcagccaga
     1321 cactcgctca gcgacagtgg ctgttgtcgt gcattgactg cgctcatcac cagctcctga
     1381 atttggcagg cgggttgtag agcccgccgg accagtcgac cagttcggcg atgctcttgg
     1441 ccgcgagcaa ttcacgactg gcatcggcac gacgaatgcc gaggtcttcc ggcagcgcga
     1501 tcagcccttc gcggcgcatg cgctcggtgt ctttcgggtt atgccgcagg ccgatggcgg
     1561 tcaccccggc gaccgcagcg atcatcgcct ggcgctcttc cagcgagcgc gccttgtaca
     1621 ggtaggcgat gccctcttcg gtgagcaggt gggtgacgtc gtcgccgtag atcatgatcg
     1681 gcgccagcgg catgccgctt ttcttcgcca cctcgaccgc gtcgagctga tcgacgaagg
     1741 tcggtttgcc gccctcctgg aaggtctcga ccatctgcac caccagcttc ttgccgcgct
     1801 cgagcagcgg ggcctcgctc tcgctcggac gcatatccaa ccaggctggc gtgggatggc
     1861 ggcggccgcg cgggtcgtgg cccatattcg gtgcgccgcc aaaaccggcc aggcggccac
     1921 gggtcacggt ggaagaatgg ccatcgccat ccacctgcag ggtggcaccg ataaacagat
     1981 ccaccgcgta ctgcccggcc agctggcaca tcatccggtt ggagcgcatc gagccgtcac
     2041 ggccggtgaa gaacacgtct ggtcgctggg cgatgtagtt ctccatgccc agctccgtgc
     2101 cgaagcagtg cacgctctcg acccaaccgg tttcgatggc cgggatcaac gtcggatgcg
     2161 ggttgagcgt ccaattgcgg cagatcttgc ccttgaggcc aagggactcg ccgtaggtcg
     2221 gcaggatcag ctcgatggcg gcggtgttga agccgatacc gtggttgagc gactgcacct
     2281 tgtgtttctc gtagatgccg cggatcgcca tcatcgccat cagcacatgc accggcttga
     2341 tatggcgcgg gtcgcgggtg aacagcggtt cgatatagaa cggcttgtcc gccaccacca
     2401 cgaaatccac ccaggaggcg ggaatgtcta cgcgcggcag ctcatcgacg atctcgttga
     2461 cctggaagat cacgatgccg tcactgaagg ccgcgggctc gaccagcgcc ggggtgtcct
     2521 cagtgcttgg cccggtgtag atattgccgt ggcggtcggc ctggaatccg gcgaccaggg
     2581 cgacgttggg gatcaggtcg accagcaggc gcgaatagag ttcgatgtag gtgtggatgg
     2641 cgccgacttc cagcaggccg tcttccagta actggctgat gcgcaggctt tgcgggccgg
     2701 cgaaggagaa gtcgagcttg cgcgcgatgc cccgctcaaa cagatcaagg tgctcggcgc
     2761 ggctgacgct gggcattatc atgtgcaggt catgcaagcg gccagggtcg acctgggcca
     2821 gagagcggga gagaaaatcc gcctgcttct ggttattgcc ttcgagcacc acccgatcgc
     2881 cgggggcgat caacgcctca agcgcggcga cgatcttgtc gctgggcagg actacaccat
     2941 ctgcaagggt gcgcacctga tcgagtcgcc gggctttttc agcgcgacga cgcgaccact
     3001 gcggcggtgg ggatattgtt gttgtcattg aagactccac gagttccgct gtcgtggagc
     3061 caccttagga gcgtgaccta gaggccatca atcaagcaaa cacatgttct attacgctca
     3121 ggttaacgat gcagttagca cggccttctc accgccctcg attaactaag cccagcagac
     3181 cgggcatact ggcgcacctg taagcaagtc ggccgcccgt tccatgcgta ttcacgtcac
     3241 ctttatcgat cgcgttggca tcacccagga agtgctggcg ctgctcggtg ggcgcaatct
     3301 caacctggat gcggtggaga tggtgccgcc caacgtctat atcgacgcgc cgaccttgag
     3361 ccccgaagtg ctcgacgagc tgcgcgaggc gctgttcaag gtgcgcggcg tacaggcggt
     3421 gaccatagtc gacatcctcc ccggccagcg ccgccacctg caactagacg ccctgcttgc
     3481 agccatgacc gacccggtat tggcggtcga cagccagggc cgcgtgctat tggccaaccc
     3541 ggcgctgatc gaactctgcg gccagcagcc cgagggtctg gggattggcg aattgtttgc
     3601 cgacagccag ttacagaccg aactactcga acagaaattc cgcctgccga tgcgcgaagt
     3661 caccctcaag ggccaggccc tgctgctgga tgcgcaaccg atcaccgaaa gcgtggacgg
     3721 cggcgcgcgg cacctggctg gcggcctgct cagcctgtat gcgccgagcc gtatcggcga
     3781 acgcctggcg gcgctgcacc atgatcacgc cgagggtttc gattcgctgc tcggcgactc
     3841 cgcaccgatt cgcaacctga aaacccgcgc ccagcgcgtc gccactctgg atgcaccgct
     3901 gctgatccag ggtgaaaccg gcaccggtaa ggagctggtg gcacgtgcct gtcacgccat
     3961 cagcacacgg cgcaccgcac cattccttgc attgaactgc gcggcattac cggagaacct
     4021 cgccgaaagc gagctgttcg gctacgcacc aggcgccttt accggtgccc agcgcggcgg
     4081 caagccgggg ctgctggagc tggccaacca gggcacggta tttctcgatg aaatcggcga
     4141 aatgtcgccc tatttgcagg ccaagctgct gcgcttttta agcgacggca gcttccgccg
     4201 ggtcggcggg cagagcgagg tcaaggtcaa tgtgcgcatc ctcagcgcca cccaccgcga
     4261 cctggaaaag atggtcaacg aaggcgcgtt ccgcgaagac ctgttctatc gcctcaacgt
     4321 actcaaccta gaagtgccgc cgctgcgcga acgcggccag gacatcctgc tgctggcgcg
     4381 gcattttatg cagcaagcct gcgcgcagat ccagcgcctg ccctgccgcc tgacgccaga
     4441 tacctacccg gcgctgctgg gcaaccgctg gccaggcaat gtgcggcagt tgcagaacgt
     4501 aatcttccgc gctgcggcga tctgcgaaag caacctggtg cagattgatg atctggatat
     4561 cgccggcacc gcagtggccg cgcagcaggc cgatggcgaa gtcagcagcc tggaagcggc
     4621 agtggaccag ttcgaaaaag ccctgctgga aaagctctac cccagccacc cctcgacccg
     4681 gcaactggcg actcggctgc acacctcgca cagcgcgatt gccatgcgcc tacgcaaata
     4741 cgggattccg ggtaagccgt aaacacgtaa atcagaatgt gccgtgacgc cccgtgccat
     4801 ccttaaagcg ctgcgcgccg gcctgggttt caccgctggc aatcaccgcc atgccgccgg
     4861 caaactcgtt gcccagcgcc tgcacaaaag gcagatccca ctgcgcataa gcgctgacgc
     4921 ggtcagcgag catgcagcgt tgtgggaagc cggcaatctc cagtgccagt tgctgcgctg
     4981 cagccaacgc gctaccggct gcgaccaccc ggttgaccag acccatgctc agcgcctcct
     5041 cggccaatac tgggcgaccg gtgagaatca gatccagcgc gcggccctgg ccgataatcc
     5101 gtggcaagcg cacggtgcca ccgtcgatca gcggcacgcc aaagcgtcgg caaaacaccc
     5161 cgagcaccgc gttattggcc atcacccgca gatcggcgag caacgccagc tccaggccgc
     5221 ctgccactgc atggccttcg atggcggcaa tcagcggctt gctcagctgc atgcggcttg
     5281 gccccatcgg tccgtcaccc tcggtttcca gacgattggc acgctcggca ccttctacca
     5341 ccgcccctag atcagcgccg gcacagaaag tcccgcccgc cccggccaat acagccaccc
     5401 gcgcctgctc gtcggcttca aaggcacgca aggcggcagc cagcgcctcg gcagtcggtc
     5461 ggtcaaccgc attgcgcacc tcggcgcggt tgatgatcag ggtggtgacc gggccgtttt
     5521 tttcgatggt cacgctcatg gcgaactcct gcgcactgtg tccgatgtag gagcggccca
     5581 tggccgcgat tttggttttc gcgggcatgg cccactccta cagctaaaaa gccgaaggcc
     5641 ggcatttagc cggccttcac cttaaagccc tgggcggtgg aaatcagcct gctgaccgat
     5701 ctccccgcca gagtggcgca atcagagggc gaacttctgc aggttggcca tcatctcttt
     5761 catcgcttcg atattgtcct tcggatgggc cgcgccctcg aagtcgcaaa tctgcgcgaa
     5821 gttagccgcc acatcttccg ggctgtagcc ttcgcgaggg tcgaaaccgg cacccaggct
     5881 gcgttcccaa cgcactttgc ccatccagcc gccgccgact tcgaacaggc cggaggtttc
     5941 ttcgcaggcg ctgctcgcca ggtacaccac cagcgggctg accagctcgg gcttgagctg
     6001 ctcaaacacc tgcggcggga tcaggccttc ggtcatgcgc gtgccgccgg taggggcaat
     6061 ggcgttgacc aggatattgt tcttgcgccc ttcgatggcc agggtgcggg tcaggccgta
     6121 caggccgagc ttggccatgc cgtagttgga ctggccgaag ttgccgtaga tgcccgaagt
     6181 ggacgcggtg aagatgatgc ggccatagcc ctgctcgcgc atatgcggcc aggcggcacg
     6241 agtgactttg taggcgcctt cgacgtgaac cttgtaaacc aggtcccagt cggcatcttc
     6301 catcttgtgg aaggtcttgt cgcgcaggat gcctgcgtta ttcaccacca catcgattcg
     6361 gccgaaggtg tccagggcgc actgcacgat cttctcgccg tcggtcaccg agtcatggtt
     6421 ggccactgcg gtgccgccag cggagcgaat ttcctcgacc accttatcgg ccgccgaagc
     6481 gttggcgcct tcaccatggg tgctgccgcc cagatcgttg accaccacct tggcaccgtg
     6541 tttagcgaac agcagcgcat gagcgcgccc cagaccgccg ccggccccgg tgacgattac
     6601 aacctgatct tgaaaatgga tggcgtcgct catgcagcat tcctcggcag attaacaatg
     6661 ctcccgagtg tccagcagtc gcgctctggt cacaataggc acgcgcagct ctgaatggtg
     6721 ctcgataagg cgacaggatg atcctgccgc cctagcgagt tttacttact gctcgccgta
     6781 accaccggta ccagtatcgg tgccagcgtc atcgttgttg gtggcgccgc cattattacg
     6841 gggcgtggta tccatgtcgt taccagtgcc attgcccgtg ccggtacccg tgccctggcc
     6901 tgtactggta ccgtccgtgc cgggtctcgg tgcctgaggg tcgttggaca ggccagggtc
     6961 gcggccatta tccatatcgg gatctgtatc ggtttcgaca ggcggaatgc ttggcggcac
     7021 cggcgaagtt gggcccgttg gatcggtagg tccgctcggc gcggc
//
LOCUS       DASRUM010000026         3515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_515187, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000026 DASRUM010000000
VERSION     DASRUM010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515187"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1312
                     /locus_tag="VFF22_02075"
     CDS             23..1312
                     /locus_tag="VFF22_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HZX15642.1"
                     /translation="MREDLNQGLIEFLKASPTPFHATASLAQRLEAAGFQHLDERASW
                     HTEAGGRYYVTRNDSSIIAFKLGKRAPLEGGIRLVGAHTDSPCLRVKPQPELQQQGFF
                     QLGVEVYGGALLAPWFDRDLSLAGRVTYRRDGKVESQLIDFQKPIAIIPNLAIHLNRE
                     ANQGWAINQQIELPPILAQLSGDEPADFRALITDQLARQHDFSADAVLDYELSFYDTQ
                     SAAVIGLNGDFIAGARLDNLLSCYAGLQALLAAPDEESCVLVCTDHEEVGSCSACGAD
                     GPMLEQVLRRVLPDGDAFVRTIQRSLLVSADNAHGVHPNYADKHDQNHGPKLNAGPVI
                     KINSNQRYATNSETAGFFRHLCLENEVPVQSFVTRSDMGCGSTIGPITASQLGVRTVD
                     IGLPTFAMHSIRELAGSHDLAHLVKVLTAFYASAELP"
     gene            1312..1710
                     /locus_tag="VFF22_02080"
     CDS             1312..1710
                     /locus_tag="VFF22_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZX15643.1"
                     /translation="MLTGSCLCGALAYSVARLDKPIGHCHCLTCQKAHAAPFATTAGV
                     LREDFRWLRGEDKLSSYESSPGKLRHFCSVCGSQLVAERPAQPHVIIRVATLDQDPGE
                     RPAQHIWTSHDRPLLATAALPSHDEWPPGR"
     gene            complement(1712..2428)
                     /locus_tag="VFF22_02085"
     CDS             complement(1712..2428)
                     /locus_tag="VFF22_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HZX15644.1"
                     /translation="MRDEAPRAVTAICRALEEQIEHGLLPSGSQLPAERKLSEVFDTT
                     RITLREALGQLEAQGLIYREERRGWFVSPPRVAYNPLVRSHFHAMVGEQGRVPATEVL
                     SAKLIPASAEICQLLALPALSSVYQIRRARRIDGRLVLYVEHYLNPNYFAGILDFDLT
                     RSLTELYASEYGIHYGRVRFDMVPTALHSEAASALKVAVGSPALRIVRINRDQHGRLI
                     DCDLEFWRHDALHVSVEVPE"
     gene            2771..>3515
                     /locus_tag="VFF22_02090"
     CDS             2771..>3515
                     /locus_tag="VFF22_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX15645.1"
                     /translation="MKQLLLASLMGSAIALATSAMAAETDLQALEKAARAEGAVNSVG
                     MPDSWANWKDTWVDLNKLYGLKHMDTDMSSAQEIAKFAAEKENATADIGDVGAAFGPI
                     AVQQGVTQAYKPSTWEQIPAWAKDTDGHWMLAYTGSIAFIVNKQLVKDIPRSWADLKT
                     GTYKVSAGDVSTAAQAVNGVLAAAIAMGCDESNVKPALEFYGELAKQGRLSLANPTIQ
                     TLEKGEVEVGVVWDFNGLSYRDQIDPERFE"
BASE COUNT          719 a         1143 c         1013 g          640 t
ORIGIN      
        1 cgattgccgt ctggagtgcc ctatgcgcga agatttgaat caaggcctga tcgagttcct
       61 caaggcctcg ccaacccctt ttcatgccac cgccagcctg gctcagcgcc ttgaagcggc
      121 cggttttcag cacctggacg aacgcgccag ctggcacacc gaggccggcg gccgctatta
      181 cgttacacgc aacgactcct cgatcatcgc cttcaagcta ggcaaacgcg cgccgctgga
      241 aggcggtatt cgcctggtcg gcgcacacac cgacagcccg tgcctgcggg tcaaaccgca
      301 accggagctg cagcaacagg gctttttcca gctcggcgtt gaggtttacg gcggcgcatt
      361 gctcgcgccc tggttcgacc gcgacctgtc gctggccggt cgggtgacct accgccgcga
      421 cggcaaggtg gaaagccagc tgatcgactt ccagaagccg attgcgatca ttcccaacct
      481 ggccattcac ctcaaccgtg aagccaacca gggttgggcg atcaaccagc agatcgagct
      541 gccgccgatc ctcgcgcagc tatccggcga tgagccggcc gatttccgcg cgctgatcac
      601 cgatcaactg gcccgccaac acgacttcag cgccgatgcg gtgctcgatt acgagctgag
      661 cttctacgac acccagagcg ctgcagtgat aggcctgaat ggcgacttta tcgccggcgc
      721 gcggctggac aacctgctgt cctgctacgc cgggctacag gcgctgctgg ccgcgccgga
      781 tgaagaaagc tgcgtgctgg tctgtaccga ccacgaggaa gtcggctcct gctcggcctg
      841 cggcgccgac ggcccgatgc tcgaacaggt attgcgccgc gtgctgccgg atggcgatgc
      901 cttcgtgcgc accattcagc gctcgctgct ggtctcggcc gacaacgccc acggcgtaca
      961 ccctaactat gccgacaagc atgaccagaa ccacggcccc aagcttaacg ccggcccggt
     1021 gatcaagatc aacagcaacc agcgctacgc caccaacagc gaaaccgccg gattcttccg
     1081 tcatctgtgc ctggaaaatg aagtgccggt gcaaagcttc gtcacccgca gcgacatggg
     1141 ttgcggctcg accatcggcc cgatcaccgc cagccaattg ggcgtgcgta ccgtggatat
     1201 cggcctaccg accttcgcca tgcactcgat ccgcgaactg gccggcagcc atgatctcgc
     1261 gcatctggtg aaggtgctga ccgcgtttta cgccagcgct gagctgcctt gatgctgacg
     1321 ggtagctgtt tgtgcggtgc gctggcctac agcgtagcgc gcctggacaa gcccattggt
     1381 cactgtcact gcctgacgtg ccagaaagct catgcagccc cgttcgctac tacggctggc
     1441 gtgctgcgtg aggattttcg ctggttgcgt ggtgaagaca agctctccag ctatgagtct
     1501 tcgccgggaa agttgcgcca tttctgttca gtctgcggct ctcagctggt ggctgaacga
     1561 cctgcccagc cccatgtgat catccgtgtg gcaacgctgg atcaggaccc cggcgaacgc
     1621 cctgcccagc atatctggac cagccacgac cgacccttgc tggcaaccgc agcgctgcct
     1681 agccatgacg agtggccgcc aggtcgctaa atcactccgg cacctcgacg ctcacatgca
     1741 gagcgtcgtg gcgccagaac tccagatcgc aatcaatcaa ccggccatgc tggtcgcggt
     1801 tgatccgcac gatacgcagc gccgggctgc ccaccgccac tttcaatgcc gacgcggctt
     1861 cgctgtgcaa cgccgtgggc accatatcga aacgcacccg gccgtaatga atgccgtatt
     1921 cgctggcata cagctcggtc agcgaacggg tcaggtcgaa atccagaatg ccggcgaagt
     1981 aattcgggtt gaggtagtgc tcgacataca gcaccagacg cccgtcgata cgccgtgcgc
     2041 ggcggatctg atagacgctg gacagcgccg gcaaggccag tagctggcag atctcggcgc
     2101 tggccggaat cagcttggcg cttagcacct cggtagccgg cacccggccc tgctcgccga
     2161 ccatagcgtg gaagtggctg cgcaccagcg ggttgtaggc cacccgcggc ggcgaaacaa
     2221 accagccgcg acgctcctcg cgatagatca acccctgagc ctcaagctgg cccagcgcct
     2281 cacgcagggt gatgcgcgtg gtatcgaata cctcgctgag cttgcgctcg gccggcaact
     2341 gactgcccga cggcaacagg ccgtgttcga tctgttcttc caatgcccgg catatagctg
     2401 tgactgcccg cggcgcttca tcgcgcatca agacctccat atttggacta gtccagcccc
     2461 attccggggc atcaacctgt gggtgcagca gtaaaaatca tgtttcaagt ttaagcagtc
     2521 ttgatgacca cgccatgaca actttctttc agcagtagca aaagctcata aaacttggtc
     2581 tgcaggccat acatgactga atttgttcat ggtctacgct agtggtgcgc cagtttgtgc
     2641 aacccggctt gagccggcaa atgaccaaca tacataaaac tgtcatttaa ccagcctaaa
     2701 ttggcctagg tcttgctgat ctagaccaga acttttcact gcacgcactt caatcgcaaa
     2761 ggagtttcca atgaaacaac tgctgctggc ttcactgatg ggctcagcca tcgccctggc
     2821 cacttcggcc atggctgctg aaacggactt gcaagcactg gaaaaggccg cgcgcgctga
     2881 aggcgcggtg aacagtgtgg gcatgccgga cagctgggcc aactggaagg acacctgggt
     2941 tgacctgaac aagctgtatg gcttgaagca tatggacacc gacatgagct cggcccagga
     3001 aatcgccaag ttcgccgctg agaaggaaaa cgccaccgcc gatatcggcg acgtaggcgc
     3061 ggccttcggc cccatcgccg tgcagcaggg tgttacccaa gcctacaaac cgagcacctg
     3121 ggagcagatc ccggcctggg ccaaggacac cgacggccac tggatgctcg cctacaccgg
     3181 ttccattgcc tttatcgtca acaagcagct ggtcaaggac atcccgcgct cctgggccga
     3241 cctcaagacc ggcacctata aagtctccgc cggcgatgtc agcaccgccg cgcaggcggt
     3301 caacggcgta ctggccgccg ccatcgccat gggctgcgat gaaagcaacg tcaaaccggc
     3361 cctggagttc tacggcgaac tggccaagca gggccgtctg tcgctggcca atccgaccat
     3421 ccagaccctg gaaaaaggcg aagtggaagt cggcgtggtc tgggacttca acggcctgag
     3481 ctaccgcgat cagatcgacc ccgagcgctt cgagg
//
LOCUS       DASRUM010000027         8242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1018128, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000027 DASRUM010000000
VERSION     DASRUM010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8242
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018128"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFF22_02095"
     CDS             complement(<1..314)
                     /locus_tag="VFF22_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017905839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HZX15646.1"
                     /translation="MQRIIKNGQLIDESWHLLPKDATLDGIPNCDDLIVPLGLWVEHS
                     TALKARDGGLGVWLDAGEEIEEIADQLDNFQVIALNFPAFTDGRHTSTAYLLRNRYGY
                     KG"
     gene            complement(298..1956)
                     /locus_tag="VFF22_02100"
     CDS             complement(298..1956)
                     /locus_tag="VFF22_02100"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HZX15647.1"
                     /translation="MYVYDQYDQKIVEDRVKQYRDQTRRYLAGELSGEEFRPLRLQNG
                     LYIQRFAPMLRVAVPYGLMSSAQVRMMAKIARDYDKGYAHISTRQNVQFNWPDLEDVP
                     DILAELATVQMHAIQTSGNCIRNTTTDQFAGVAKDEIIDPRPWCEIIRQWSTFHPEFT
                     HLPRKFKIAVNGAVSDRAAIEVHDIGLEAVQNDAGELGFRVSVGGGLGRTPIVGSFIN
                     EFLPWQHLISYLDAILRVYNRYGRRDNKYKARIKILVKALTPDVFAERVNAEWAHLKD
                     GPSTLTEAEVARVSAHFIDPAYKALADQDAALAALDAEHPGFARWRQRNTFAHKKPGY
                     VAVTLSLKPTGVAPGDVTDKQLDVIAELADRYSFGEVRNSHNQNIILADVEQAQLFTL
                     WGELRENGFATPNVGLLTDIICCPGGDFCSLANAKSIPIAEAIQRRFDDLDYLFDIGN
                     IDLNISGCMNACGHHHVGHIGILGVDKKGQEFYQVSLGGSAGRDASLAQILGPSFAEA
                     DMADVIDKIVKVYVERRDEEESFLDTFRRIGVDPFKERVYAANH"
     gene            complement(2389..3006)
                     /locus_tag="VFF22_02105"
     CDS             complement(2389..3006)
                     /locus_tag="VFF22_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15648.1"
                     /translation="MSDMEHQEVDLSKPQNQDLIWDLDSIARRELAERFIKLFENRLC
                     VYSESTRQLYTNYDLHFPSDMGRKMVVLPNPYAFHDTLHGIESHAVRKTGLCVLPGVV
                     LRKPGLLLTTMIKEGGPAPKTMPFKPALAQIISNQKKAGDIFLPIMMKGDLREFDQQM
                     PYIHLHRLQVSRLTRLSTFERDDIQQSITRKLLMLYRQADSLSCH"
     gene            complement(3014..4648)
                     /locus_tag="VFF22_02110"
     CDS             complement(3014..4648)
                     /locus_tag="VFF22_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016704793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX15649.1"
                     /translation="MNSLRSLPINRRLWLILVVAIVMLIIQGALLLKQIHADLYAAKA
                     EKTQHVVQSAAGILQHYHALEAAGSMPREEAQKQAMKTIRGLRYDGQEYFWINDQTPV
                     MVMHPTNPKLEGQNLSGFKDPDGKALFNEMVAISKSQGAGQVDYRWPKPGASDPVPKV
                     SYVQLFQPWGWIIGSGVYVDDVQAEFQAQALKAMVIGLVIAALVALLIVLIVRSITQP
                     LQQAVSAMANIASGDGDLTRNLDIQGNDELSALSRHFNAFTDKLRLVIKQSLDSAGQL
                     DTAARNLGQVSGQAQQHSEQQSQQMELVATAINEVTYGVQDVAKNAEHASSEVHAAEE
                     QALQGQQSIEASLRQINELSGTIDQAVAVIQALAQESTQIGSVLEVIRSIAEQTNLLA
                     LNAAIEAARAGEQGRGFAVVADEVRLLAQRTQKSTAEIQVMIERLQGNSEAAVKVIND
                     SSKASQLTIEQASQAGASLTLIASGLRNLTGLNASIASATLQQSHVVEDINQNVTQAA
                     SLAHNSALAAQQSSEAGQHLGQLAEQLNRLLGQFRV"
     gene            4981..5811
                     /locus_tag="VFF22_02115"
     CDS             4981..5811
                     /locus_tag="VFF22_02115"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017528990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HZX15650.1"
                     /translation="MDLWVAVQALILGVVEGVTEFLPISSTGHQIIVADLIGFGGERA
                     MAFNIIIQLGAILAVVWEYRRKILEIALGLPKEPQARRFTTNLLIAFLPAVALGVLFA
                     DFIHHYLFNPITVALALVVGGVIMLWAERREHLVHAESVDDMTWKDALKIGFAQCLAM
                     IPGTSRSGATIIGGLLFGLSRKAATEFSFFLAMPTMVGAAVYSGYKYRDLFQSSDLPV
                     FAIGFVTSFIFAMIAVRALLKFIGNHSYAAFAWYRIGFGLLILATWQLQWIDWSTAQQ
                     "
     gene            5897..6865
                     /locus_tag="VFF22_02120"
     CDS             5897..6865
                     /locus_tag="VFF22_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015474865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX15651.1"
                     /translation="MHQLEHQLLQVNGIDLSLYSAGPATGRPVWLLHGFPECWHSWRQ
                     QISPLADAGYRVLIPEMRGYGQSSAPRDPAAYDVLTICADIQAAMDQLGQQQVCVIGH
                     DWGAPIAWHLTLLEPSRVQAVVGMAVPFGGRPRQPAIEIMRQLYAERFHYIVYFQEPG
                     VAEAEMDADIPRTLRMMMHNSSAAVPRDHFLQDKPAGAGLFDGMQDPGTLPGWCDSAA
                     FDHYLKTFEGRGFYGALNWYRNFERNWQRTEALAERQIEQPALFLLGDKDPVGTLEAY
                     TLKQMPKRIAQLEQHVLQNCGHWIQNEQAEQVNQMLLDFLARHFPS"
     gene            complement(6893..7792)
                     /locus_tag="VFF22_02125"
     CDS             complement(6893..7792)
                     /locus_tag="VFF22_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZX15652.1"
                     /translation="MHDLNDLFYFAKVVEAGGFAAAGRTLGIPKSRLSRRIAELENRL
                     GARLLQRTTRKLALTDIGERYLRHCQAMLQEAEQAEETVASLTSEPRGRLRVSAPGGI
                     ALLSELVVSFLAAYPKVQLELIQTNRRIDLLNEGVDVALRVRSAEDEDPTLITRRLRP
                     AQAFMVAAPALLQHAKVSTPDDLSALPALGALEADRRIHLRLQHDSGRVQEVVLEARL
                     AIEDFNVRKHAAMTGLGFTMLPAPLCLDELADGRLIRLLPDWALPTGNLQAVYTHRRG
                     MLPAVRAWIEHISDAFSRWDQPI"
     gene            7916..>8242
                     /locus_tag="VFF22_02130"
     CDS             7916..>8242
                     /locus_tag="VFF22_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010953771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZX15653.1"
                     /translation="MKLLHLDSSILGDSSASRQLSRAVVDAWLVAEPEAQVSYRDLAS
                     DALSHLSAASLVAGGTPAELRDAAQKHEVELAEGTLNEFLAADAIVIGAPMYNFTVPT
                     QLKAWID"
BASE COUNT         1497 a         2327 c         2568 g         1850 t
ORIGIN      
        1 tcgcctttgt agccgtagcg gttacgcagc aggtaggcgg tggaggtgtg gcgtccatca
       61 gtaaaggccg ggaagttcag cgcgatgacc tggaagttat ccagctgatc ggcgatttcc
      121 tcgatttcct caccggcgtc cagccacacg cccaggccgc catcacgggc cttgagcgcg
      181 gtgctgtgct cgacccacag gcccagcggc acgatcaggt cgtcgcagtt gggaatgccg
      241 tccagcgtgg cgtccttggg cagcaggtgc cagctttcat cgatcagctg gccgttctta
      301 atgattcgct gcatatacgc gctccttgaa cgggtcgaca ccaatacggc ggaaggtgtc
      361 gaggaaactc tcttcttcat cgcggcgctc aacatagact ttgacgatct tgtcgatcac
      421 atcggccatg tccgcttcgg cgaaggaggg gccgaggatc tgcgccaggc tggcgtcacg
      481 accggcgctg ccgccgaggg acacctgata gaactcctgc cccttcttgt ccaccccgag
      541 gatgccgata tggccgacgt ggtggtgacc gcaggcgttc atgcagccgg agatgttcag
      601 gtcgatattg ccgatgtcga acaggtaatc caggtcgtcg aaacgacgct ggatggcttc
      661 ggcaatcggg atcgacttgg cgttggccag ggagcagaag tcgccgcccg ggcagcagat
      721 gatatcggtc agcaggccga cgttcggcgt ggcgaaaccg ttttcacgca gctcgcccca
      781 cagggtgaac agctgcgcct gctcaacatc cgccaggatg atgttctggt tgtggctgtt
      841 gcgcacttcg ccaaagctgt agcggtcggc cagttcggcg atcacgtcga gctgcttgtc
      901 ggtgacatcg cctggagcca cgccggtcgg cttgagcgac agggtgacgg cgacatagcc
      961 cggcttcttg tgggcgaagg tgttgcgctg acgccagcgg gcaaagcccg ggtgttcagc
     1021 atccagtgca gccaaggcgg cgtcctgatc ggccaaggct ttgtaggctg ggtcgataaa
     1081 gtgtgcagac acgcgcgcca cttcagcttc ggtcagagtg ctggggccat ctttcaggtg
     1141 agcccattcg gcgttaacac gctcggcgaa cacgtccgga gtaagggcct tgaccagaat
     1201 tttgatgcgc gccttgtatt tgttatcacg gcggccatag cggttgtaca cacgcaggat
     1261 ggcgtcgagg taactgatca ggtgctgcca cggcaggaat tcgttgataa agctgccgac
     1321 gatcggggta cggcccaggc cgccaccgac ggacacgcgg aagccgagct cgccggcgtc
     1381 gttctgtacc gcttccaggc cgatgtcgtg cacctcaatc gctgcacggt cgctcactgc
     1441 accattaacg gcaatcttga acttgcgcgg caggtgggtg aactcggggt ggaaggtcga
     1501 ccactgacgg atgatttcgc accacgggcg tgggtcgatg atttcatcct tggccacacc
     1561 ggcgaactgg tcggtggtgg tgttacggat gcagttgccg ctggtctgga tcgcgtgcat
     1621 ctgcacggtg gccagctcgg cgagaatgtc gggcacatct tccaggtccg gccagttgaa
     1681 ctggacgttc tggcgagtgc tgatgtgcgc gtaacccttg tcgtagtcgc gggcaatctt
     1741 ggccatcatg cgcacttgtg cagacgacat caggccgtac ggcacggcaa cgcgcagcat
     1801 gggcgcaaag cgctggatgt acaggccatt ctgcaggcgc agcgggcgga attcttcacc
     1861 gctcagttcg ccagccaggt aacggcgggt ttgatcgcgg tactgcttga cgcgatcttc
     1921 gacgattttt tggtcgtact ggtcgtatac gtacatgtag tgtcctgtta tcaggctttt
     1981 tacagctaat cagcgcgcac ggccgcgcag tcctggcgga ggcgaaggaa gataccagct
     2041 cagggttatg cgcaaaagtg atgtttgagt atatggaaag aacttaaaga gataagacgg
     2101 ccattgaaca tagccctatg gcttgagtaa gcgggcatgc tggtcttaac tgcacaagac
     2161 gattcaccca taaacacaac aagaggggta gtccatgagc gaacacagtg aaccggataa
     2221 tgctgacagc gcggtcgatg ccaaggccat ttttgccttg attgtgatcg tcgtcgtcac
     2281 cgccgtgttc tgggtcagcc agcaataaga cccagttggt ggctaaagca caaagcggga
     2341 tcagttaact gatcccgctt tttattgtcc gcagagcagg gttggcagtc agtggcagct
     2401 aaggctgtcg gcctggcgat agagcataag caacttacgc gtaatggact gctgaatgtc
     2461 gtcgcgctcg aaggtcgata gtcgcgtcag gcgactgacc tgcaggcggt gcaggtggat
     2521 atagggcatc tgctgatcga actcgcgcag gtcgcccttc atcatgatcg gcaggaagat
     2581 atcgccggcc ttcttctggt tgctgatgat ctgcgccagc gccggtttga acggcatggt
     2641 tttcggcgca gggccgcctt ccttgatcat cgtggtcagc agcaagcctg gcttacgcag
     2701 aaccacgccg ggcagcacgc acagtccggt tttacgtacg gcatgcgact cgatgccatg
     2761 cagggtgtcg tggaacgcat agggattggg tagcaccacc atcttgcggc ccatatcgct
     2821 ggggaagtgc aggtcgtagt tggtgtacag ctggcgcgtc gactccgaat acacgcacag
     2881 gcggttctcg aacaacttga taaagcgttc cgccagctcg cgccgggcaa tgctgtccag
     2941 gtcccagatc aggtcctggt tctgcggttt gctcaggtcg acttcctggt gttccatatc
     3001 gctcattgcg ttctcatacc ctgaactgtc caagcagccg gttgagctgc tcggccaact
     3061 gacctaggtg ttgcccggcc tcactggatt gttgcgccgc caaagcactg ttatgggcca
     3121 ggctggctgc ctgggtgaca ttctggttga tgtcttcgac cacgtgcgat tgttgcagtg
     3181 tcgcgctggc gatggaagca ttcaaaccgg tgagattgcg cagcccactg gcgatcaacg
     3241 tcaggctggc gccggcctgg ctggcttgct cgatggtcag ctgcgaagct ttgctgctgt
     3301 cgttaataac cttcaccgcg gcctccgagt tgccttgcag gcgctcgatc atcacctgga
     3361 tttccgctgt ggatttttgc gtgcgctgcg ccagcaggcg cacctcatcg gccaccacgg
     3421 caaagccacg gccttgctcg cctgcgcggg cggcttcgat ggcggcattc agcgccagca
     3481 gattggtttg ttcggcaatc gagcggatca cttccaatac gctgccgatt tgcgtgcttt
     3541 cctgggcgag ggcctgaatc accgccacgg cctggtcgat ggtgccggaa agctcgttga
     3601 tctgccgcag gctcgcctca atgctctgtt ggccttgcag ggcttgttct tcggccgcgt
     3661 ggacttcgga tgaggcatgt tcggcgttct tcgccacatc ctgcacgcca taggtcactt
     3721 cattgatcgc ggtggcgacc agctccatct gctgcgactg ctgctcgctg tgctgctgcg
     3781 cctgaccgga aacctgaccg aggttgcgcg cagcagtatc gagttggccg gcagaatcga
     3841 gggactgctt gatcaccaga cgcagcttgt cggtgaaggc attgaagtgc cgggacaggg
     3901 cagacagttc atcgttgcct tggatgtcga gattgcgcgt caggtcgcca tcgccgctgg
     3961 cgatattggc catggcactg acggcttgct gcagcggctg ggtgatgctg cgtacgatca
     4021 gcacgatcag cagggccacc agcgcggcaa tcaccaggcc gatcaccatg gcttttagcg
     4081 cctgggcctg aaactcggcc tgtacatcgt cgacataaac gccagagccg atgatccagc
     4141 cccagggctg gaacaattgc acgtaagaca ccttgggcac gggatcgctt gcgcccggtt
     4201 ttggccagcg gtaatcgacc tgaccggcgc cctggctttt acttatggcg accatttcat
     4261 tgaacagcgc cttgccgtca gggtccttga aacccgagag attctggcct tccagtttgg
     4321 ggttggtcgg gtgcatgacc atcaccgggg tctggtcatt gatccagaag tattcttggc
     4381 cgtcgtagcg caggccgcgg atggttttca tcgcctgctt ctgcgcctct tcgcggggca
     4441 tgctgccggc agcttccagg gcgtgatagt gctgcagaat gccggcggcg ctctgtacca
     4501 cgtgttgagt tttctcggct ttggcggcgt acagatcggc gtggatctgc ttgagcaaca
     4561 atgcgccctg aataatcagc atgacgatgg cgaccaccag gatcagccat aggcgacggt
     4621 taatgggcag gctgcgcagg ctattcatgg ggtgtctccc ttattgttgt atgcctctgt
     4681 atcggccaag agcgtttttt gactaggtta gaccttaggc tagctagcag ataagcgaag
     4741 tgtaagtgag cgattccatc ggctctctcg gcgtgagcgt tttgcttgcc ttcgtggggc
     4801 tcgcactaaa tgtgcagctt ctctgctagg atttgcaccg ttgcgtgtta agtgggtgat
     4861 ttaattattt tttctgtccc cgaggaacct tttctagagg gttttgtcgg acgccctcgc
     4921 aagttcatca tcatatttat tgttgttcgt gccggataaa gcacgcattt aggagtggag
     4981 atggaccttt gggttgccgt acaggcactg attctgggtg tcgttgaagg tgttaccgag
     5041 tttctgccga tttccagtac cgggcaccag attatcgtcg ccgacctgat tggttttggc
     5101 ggcgagcgag cgatggcctt caatatcatt atccaacttg gggcgattct cgcggtggtg
     5161 tgggagtacc gacgcaagat tctcgaaatt gcgctgggcc tgcccaaaga gccacaggcg
     5221 cggcgcttca caactaatct gctgattgcc ttcctacctg ccgtggcgct gggtgtgctg
     5281 tttgccgact ttatccatca ttacttgttc aacccgatca ccgtggccct ggcgttggtg
     5341 gtgggcgggg tgatcatgct ctgggccgag cggcgcgagc atctggtaca cgctgagagc
     5401 gtggatgata tgacctggaa ggatgcgctg aagattggtt ttgctcagtg cctggcaatg
     5461 attccgggta cctcacgctc cggcgcgacg atcatcggcg gcctgttgtt tggtctgtcg
     5521 cgcaaggctg caaccgagtt ttccttcttt ctcgccatgc cgaccatggt cggcgctgcg
     5581 gtgtactccg gctacaaata ccgtgacctg ttccaatcca gtgatctgcc ggtgtttgcg
     5641 attggttttg tcacctcgtt tatcttcgcc atgattgccg tgcgggcgct gcttaaattc
     5701 attggtaacc acagttatgc ggcgtttgcc tggtaccgca tcggttttgg cttgctgatc
     5761 ctggcaacct ggcagctgca gtggattgac tggagcacgg cgcagcaata attgctgatc
     5821 ccgtattcgg cagataaata gaggcgcacc ccggggtgcg cctttgtcat agtggtctcg
     5881 attattggag gcgcacatgc atcagctcga gcatcaactt cttcaggtca acggtatcga
     5941 cctcagcctt tacagcgccg gccccgctac ggggcggccg gtctggttgc tacatggctt
     6001 tcccgagtgc tggcattcct ggcgccagca gatttcgccg ctggcggatg ccggttaccg
     6061 cgtgctgatc ccggaaatgc gtggctatgg ccagagcagt gcgccacgtg atccagcggc
     6121 ttacgacgtg ctgaccatct gcgctgatat ccaggcggcc atggaccagc ttggccagca
     6181 gcaggtgtgt gtgatcggcc acgattgggg cgcaccgatt gcctggcatc tgaccttgct
     6241 tgagccttcg cgggtgcagg cggtcgttgg catggccgtg ccgtttggcg gacggcccag
     6301 gcagccggcc atcgagatca tgcgccagct gtacgccgag cggtttcatt acattgtgta
     6361 tttccaggag cctggtgtcg ccgaggcgga gatggatgcc gacatcccgc gcaccttgcg
     6421 catgatgatg cacaacagct cagcggccgt gcctagggac cactttctgc aggataaacc
     6481 cgccggtgct gggttgttcg atggcatgca ggaccctggc acactaccgg gctggtgcga
     6541 cagcgcagca tttgatcatt acctgaaaac cttcgagggt cgtggcttct acggcgcgtt
     6601 gaactggtac cgcaattttg aacgcaactg gcagcgcact gaggctctgg cggaacggca
     6661 gatcgagcag cccgcgctgt ttctgctggg tgataaggac ccggtcggca ccctggaggc
     6721 ttacaccctc aagcagatgc ctaagcgcat cgcccagttg gaacagcatg tgctgcagaa
     6781 ctgtgggcac tggattcaga acgagcaggc ggagcaggtt aatcagatgc tgctggattt
     6841 tctcgctcgg cattttccca gttaaaccac aggcttcgtt aatagtgcat agctagatcg
     6901 gctgatccca gcggctaaag gcatcactga tatgttcgat ccatgcccgc acggcgggca
     6961 gcataccgcg tctgtgggtg tataccgcct gcaggttgcc ggtaggcagc gcccagtcag
     7021 gcagcaggcg gatcagtcgg ccatcagcca gttcatccaa gcacaggggc gcgggcagca
     7081 tggtaaaacc caggccagtc atcgccgcgt gtttgcgcac gttgaagtcc tcgatggcca
     7141 ggcgcgcttc cagcacaact tcctgcacgc gcccgctgtc gtgctgcaag cgcaggtgaa
     7201 ttctgcggtc tgcttctaac gctccgaggg ccggtagtgc tgagaggtcg tcaggtgtgc
     7261 tgaccttggc gtgctgcagc aaggccggtg cggcgaccat aaaggcctgg gctggacgca
     7321 ggcggcgggt gatcagcgtg gggtcttcat cctcggcgct gcgcacgcgc agggcgacgt
     7381 ctacgccttc gttgagcagg tcgatacgcc gattggtctg aatcagctcc agctgcacct
     7441 tggggtaggc cgcgagaaag ctcaccacca gctccgagag cagggcaatg ccgcctggcg
     7501 cgctgacccg caggcgcccg cgcggctcgc tggtcaggct ggccacggtt tcctcggcct
     7561 gttcggcctc ctgcaacatg gcctggcaat ggcgcaggta acgctcgccg atatcggtca
     7621 gcgccagttt gcgcgtggtg cgttgcagca ggcgtgcgcc gaggcggttt tccagctcgg
     7681 caatccgtcg tgacaggcgt gattttggaa tgcccagggt gcgcccggcg gcggcaaagc
     7741 cgccggcttc caccactttg gcgaagtaga acaggtcatt gaggtcgtgc atggctttat
     7801 cgtcctatca gtgggacgaa ctatcgcatt tgcgctgact aatcggctat tgctttcatc
     7861 gatagtatct ccagcacctt gatcgccgtg gggcgatctc aatttggaga ttgtcatgaa
     7921 gctactgcac ctcgattcca gcatcctcgg cgactcgtcc gcctcccgcc aactcagccg
     7981 tgccgtggtc gatgcctggc tcgtcgccga acctgaagcg caggtgagct accgcgacct
     8041 ggccagcgat gcactgagcc atctgtccgc cgccagcctg gtcgccggtg gtacacctgc
     8101 ggagctgcgc gatgcggcgc aaaagcatga agtcgagctg gcggaaggca ccctcaacga
     8161 gtttctcgcg gccgatgcca tcgtgattgg cgcgccgatg tacaacttca ccgtgccgac
     8221 ccagctcaag gcctggatcg ac
//
LOCUS       DASRUM010000028         9470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_516154, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000028 DASRUM010000000
VERSION     DASRUM010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9470
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516154"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VFF22_02135"
     CDS             <1..599
                     /locus_tag="VFF22_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX15654.1"
                     /translation="GGQAIAVQADVADESQVIQLFARLDQHFGRLDVLVNNAGMLETQ
                     MRLQQMDLARWQRVFATNVFGSFLCCREAIKRMSTRQGGNGGAIVNLSSAAARLGAPG
                     EYIDYAAAKGAIDSMTLGLAKEVAADGIRVNAVRPGVIETEIHASGGEPQRVARVSQS
                     VPMGRGGLASEVAEAILWLASEQASYTSGALLDVSGGR"
     gene            complement(609..1589)
                     /locus_tag="VFF22_02140"
     CDS             complement(609..1589)
                     /locus_tag="VFF22_02140"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HZX15655.1"
                     /translation="MQIALAPMEGLVDEILRDVLTRVGGIDWCVTEFIRVTERVLPVS
                     SYEKLAPELFSGAQTAAGTPLRVQFLGSDPACLADNAAFACTLGAPVIDLNFGCPAKT
                     VNKSRGGAVLLKEPELLHAIVREVRRAVPAAIPVTAKMRLGFDHKDYALECAQALADG
                     GAAQIVVHARTKVEGYKPPAHWEWVARVQEAVKVPVFANGEIWTLDDWRRCREVSGVE
                     DIMLGRGLVARPDLARQIAAAKAGQDVAPMSWVELQPLLADFWLQARRKMSPRYAPGR
                     LKQWLAMLTRSYPEATELFNLLRRESDCLRIDGLLGIEFIEPERAEQASA"
     gene            1584..2099
                     /locus_tag="VFF22_02145"
     CDS             1584..2099
                     /locus_tag="VFF22_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15656.1"
                     /translation="MHKQALEKTLRSVRGEPAGASVKWNTVRLGESTSRPLTVLETIM
                     RTLLLLGCLYLSLGLSHAEPATQAPTANTGAKLAQLIIGRDEKVPKTCDVELQLEEKT
                     VAQLQAGDSISLDIPAGTHYLRAKLLAAADCTADDLASGQSVLLEPGETRQYQVMLDN
                     NALFLAPQLKE"
     gene            complement(2107..2556)
                     /locus_tag="VFF22_02150"
     CDS             complement(2107..2556)
                     /locus_tag="VFF22_02150"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZX15657.1"
                     /translation="MKWDLSNPFVIDIQVTAEDIDGLGHANNAVYVSWLERCAWRHSQ
                     FLGLDLTEYRRLDRAMAVLRHEIDYLASAYEDQQLQMATWIVESDQRLKMDRRFQLIR
                     PEDGVTLLRAKTTFVCIELSSGKPKRMPPEFVEGYGAALQPPFPMEL"
     gene            complement(2553..3203)
                     /locus_tag="VFF22_02155"
     CDS             complement(2553..3203)
                     /locus_tag="VFF22_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HZX15658.1"
                     /translation="MSLTMDVVLQQARPVLPVLVIEDISLAVDLARALHAGGIRVLEV
                     TLRTPRALDALAAMRKELPDLLIGAGTVIHAEQFLEARDAGAQFAVSPGCTERLAAAA
                     EESGLPYLPGVMTPSEVLLALEYGYRSLKLFPADGATSIKMLKSFKGPFTGIRFCPTG
                     GVTPDNLLGFLRLPNVACVGGTWIAPSNLVRARAWDQITQLAAEARALAGSLEQQA"
     gene            complement(3240..5048)
                     /gene="edd"
                     /locus_tag="VFF22_02160"
     CDS             complement(3240..5048)
                     /gene="edd"
                     /locus_tag="VFF22_02160"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HZX15659.1"
                     /translation="MHPVVEQVTAALEKRSAKRRERYLQRLALAATRATGKALSCSNL
                     AHALAAQPDDARLIMKQGGSPHVAIISSYNDLLSAHAPLRDYPERLKVALAKAGATSQ
                     FAAGVPAMCDGITQGEGGMQLSLFSRDLIAQATAIGLTHGIFDGALYLGVCDKIVPGL
                     LIGALHFGHLPAVFVPAGPMQSGLPNKEKAAVRQRYASGEASRDELLAAELAAYHQAG
                     TCTFYGTANTNQLLMEAMGLHVPGSAFIHPYTPLRDAMTDEAARLVAHNSRQGERYLP
                     VGQQIDARALINAVVALLASGGSTNHTLHLPAIARAAGYELSWDDFAALSRVVPLLAR
                     VYPNGAADVNQFQAAGGPAWLLRELLGAGLLHNDVATAAGHGLRAYTREPWLDGERLA
                     WRDLPAQSPAPDIVRHVAEPFAEEGGLVLLAGNLGRAILKTSAVDPAFWQIRAQARIF
                     DSEAAVQAAYSAGELQGDLVLVVRFQGPQANGMPELHKLMPLLANLQQAGQRVALITD
                     GRMSGASGQVSTALHLTPEAAAGGALARLQDGDWLVLDAQQGLLQVELSEQQLAARPL
                     ASASAELELGYGLEMFAAQRRLVGSAEQGASSLFEE"
     gene            complement(5153..5833)
                     /locus_tag="VFF22_02165"
     CDS             complement(5153..5833)
                     /locus_tag="VFF22_02165"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HZX15660.1"
                     /translation="MSQNLLHCHAQRDACLQALASDLLAQIQATLAQQPRAGLILPGG
                     SSPQALLPLLAQQLDSQRIDLSPSDERWVPAQAAESNWQLLHQGLPQANCLDPRQGEA
                     LAQAALSWGEQLMQWSPFSAVLLGMGEDGHIASLFPAMPGLLAALDPDAEPAALPALA
                     PQEPRERLSLNLAMLQRGQWLGLLAFGERKRALIDAVLADQPSARELPLYAWLRRSPL
                     PVHIYWAP"
     gene            5934..7790
                     /locus_tag="VFF22_02170"
     CDS             5934..7790
                     /locus_tag="VFF22_02170"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZX15661.1"
                     /translation="MLLRVLLLLLLPAMTQATSLPLQDSDSPLLPGPAMQVWTDPEGL
                     AGIHSVRQLPESAWQPVARRDASFGYSTHAYWLRFTLHNPADEAINWVLLIGNPLLDF
                     LDAYGLDGERVYRAGDQRPFDERWVNHRQLVLPFSLAAGEERELILRMQTNGSANLSA
                     SLMSAQAFNQHEQRMLLLQGLFFGALLVMMIYNLSIFIITRDRNYLWYSLFVASFSLY
                     QFIQLGFALQWFWPESLAWQQMSFPFSSALATLFGVLFAHGVLDLKNQHPAYSWICRG
                     LIGSSLLVMVLALVAPYLVALIGSFVLLLVCAIFGCIFTVLRWRDGYPPAKLFALGWS
                     ALIIASLFSVLSGTGLIANSLLTLHAQQIGSLLELVIFSIALGSRIRLAQHAQQLAQE
                     QLYANEHQLRLEQAKSLDLQQQINEGLEARVEERTASLQKALQELSTANRQLAELSRR
                     DSLTGLFNRQVLNEELERMLAQAKRSQQPIAMLMMDLDHFKKVNDQHGHLVGDACLQH
                     AALRMQQRMRSNDLLVRFGGEEFAAVLCDTNQSGAMDLAEQLRADLASHPCMHQGLSI
                     DLSLSIGVYALIPDIGSDREQLLRHADQALYRAKAAGRNKVIGYEASALGDV"
     gene            complement(7760..8599)
                     /locus_tag="VFF22_02175"
     CDS             complement(7760..8599)
                     /locus_tag="VFF22_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZX15662.1"
                     /translation="MNSMSWVRGFNASIGLLAPYALASKLRREFMTPHTLPPRDWELP
                     LLAQAERITLRFGLSALRWGSGPAVLLMHGWEGRPTQFAELIKALVNAGYGVVALDAP
                     AHGRSPGREANVVLFARALLEAASELPPLKAVIGHSMGGASALLATQLGLRTDALVSI
                     AAPSRILTMLRRFSRYMGLPRQARAHFVRLVEEKAGIPAGQLDSAHYQLDFPGLVVHA
                     VDDPMVPFSEAEAIHQSWFDSRLLRLESGGHQRVLADPRVVQAVLELLKSLSQTSPKA
                     LAS"
     gene            complement(8610..9149)
                     /locus_tag="VFF22_02180"
     CDS             complement(8610..9149)
                     /locus_tag="VFF22_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009043017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZX15663.1"
                     /translation="MSEKKAHTRDRILAAASAALLQRGPAEPSVGEVMGAAGLTVGGF
                     YAHFESKDALMLEVFQQMLCQRRERVAQFDPALPAHERRTLLAAFYLSRKHRDASEQG
                     CPLPSSVGELARMPDGFRGVLAEHIELMTAQLAGSPEEADQALADIALMVGGLALARA
                     LGPGELSDRVLRAAKSAIV"
     gene            complement(9391..9466)
                     /locus_tag="VFF22_02185"
     tRNA            complement(9391..9466)
                     /locus_tag="VFF22_02185"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9430..9432),aa:Glu,seq:ttc)
BASE COUNT         1886 a         3119 c         2829 g         1636 t
ORIGIN      
        1 cgggcggcca ggccattgcc gtgcaggcgg atgtggcgga tgaaagccag gtgatacagc
       61 tgttcgcccg cctcgatcag cactttggcc gcctcgatgt actggtcaac aacgccggca
      121 tgcttgaaac ccagatgcgt ttgcagcaga tggacctggc gcgctggcag cgggtgtttg
      181 ccaccaatgt gttcggcagc tttctctgct gccgtgaggc gatcaagcgc atgtcgacgc
      241 gtcagggcgg taacggcggt gccatcgtca acctgtcctc ggcagccgca cggctcggcg
      301 cgccagggga atatatcgat tacgcagcgg ccaagggtgc gatcgacagc atgaccctgg
      361 gtctggccaa ggaagtggcg gccgacggta ttcgcgtcaa cgccgtgcgc cctggggtga
      421 ttgaaaccga gattcacgcc agcggcgggg agccgcagcg ggtcgcgcgg gttagccaga
      481 gtgtgcctat gggccgtggc ggtctggcca gtgaagtggc tgaagcgatt ctctggctgg
      541 ccagcgagca ggcgtcctac accagcggcg cgctgttgga tgttagcggc gggcgctgaa
      601 tatcaggatc aggcgctcgc ctgctcggca cgctcaggct cgataaactc gataccaagc
      661 aagccatcga tacgcaggca atcgctttcg cggcgtagca gattgaacag ctcggtggcc
      721 tcggggtagc tgcgggttag catcgccagc cattgtttga gccgacccgg tgcatagcgt
      781 ggcgacatct tgcgccgcgc ctgcagccag aaatccgcca acagcggctg cagctcgacc
      841 cagctcatgg gcgcaacatc ctgccctgcc ttggctgcgg caatctgccg ggccagatcc
      901 ggacgcgcga ctaggccgcg acccagcatg atgtcttcaa ccccgctgac ctcacggcaa
      961 cgccgccagt catccaaggt ccagatctca ccattggcga ataccggcac cttgactgct
     1021 tcctgcaccc gcgccaccca ctcccaatgc gccggcggct tgtaaccttc gaccttggtc
     1081 cgcgcgtgca ccacgatctg cgccgcgcca ccatcagcca gggcctgggc gcattccagg
     1141 gcgtaatcct tgtgatcaaa gcccagacgc atttttgccg taaccggaat ggccgccggt
     1201 accgcacggc gcacctcgcg gacgatggcg tggagcaact cgggctcctt gagcagcacc
     1261 gcgccgccac gggatttgtt taccgtcttg gccgggcaac cgaagttgag atcgatcact
     1321 ggtgcgccga gggtgcaggc aaaggcggcg ttatccgcca ggcaggccgg atcggagccg
     1381 aggaactgca cgcgcagcgg cgtgccagcg gccgtttgcg cgccgctgaa caattccggt
     1441 gcgagtttct cgtagctgct gacgggtaac acacgctcgg tcacccgaat aaattcggtc
     1501 acgcaccagt cgatgccgcc aacccgggtc agcacatcgc gaaggatttc gtcgaccagc
     1561 ccctccatgg gtgccaaggc gatttgcata aacaagctct cgaaaaaacg ctgcgcagtg
     1621 tacgcggcga accggccggg gcgtcagtaa aatggaacac cgtccgtctc ggggagtcca
     1681 catccagacc attgactgtt ctggagacga tcatgcgcac cctgctattg cttggctgcc
     1741 tgtatcttag ccttggccta agccatgcag aacccgccac gcaagccccc acagcgaaca
     1801 ccggcgcaaa gctggcgcaa ctgatcatcg gccgtgatga gaaggttcca aaaacctgcg
     1861 atgtggagct gcaactggaa gaaaaaaccg tggcgcaact acaggccggc gactcgataa
     1921 gcctggacat acctgcaggc acccactatc tccgagccaa gctgttggct gcggctgatt
     1981 gtacggcgga cgatctggcc agcggtcagt cggttctcct tgaacccggc gaaacccggc
     2041 agtaccaggt gatgctagac aacaacgcgt tgttcctggc gccgcagcta aaggaatgac
     2101 tgcgcctcag agttccatgg ggaacggtgg ctgcagcgcc gcaccatagc cttcgacgaa
     2161 ctctggcggc atgcgtttgg gcttgccgct ggacagctcg atgcagacaa aggtggtttt
     2221 tgcgcgcaac agggtgacgc catcttccgg acgaatcagc tgaaagcgcc gatccatctt
     2281 caggcgctga tcagattcga caatccaagt ggccatctgc agctgctggt cttcgtaggc
     2341 actggctagg tagtcgatct catggcgcag cacggccatg gcgcggtcca gccggcggta
     2401 ttcggtcaga tccaggccga gaaactgcga atggcgccag gcacagcgct ccagccagct
     2461 gacatacacc gcattgttgg catggccgag accgtcgata tcctctgcag tgacctgaat
     2521 atcgatgaca aaggggttgc tcaggtccca cttcatgcct gctgctccag gctgccagcc
     2581 agggcgcggg cctcggcggc cagctgggta atctggtccc aggcgcgggc gcgcaccagg
     2641 ttgctcggcg cgatccaggt gccaccgaca caggccacgt tgggcaagcg caggaagccc
     2701 agcaggttat ccggcgtcac cccgccggtt gggcagaagc gaataccggt aaacggacct
     2761 ttaaagctct tgagcatctt gatgctggtg gccccgtcgg ccggaaacag cttcagcgag
     2821 cggtagccgt attccagcgc cagcagcacc tctgacggcg tcatcacgcc cggcaggtac
     2881 ggtagaccag actcttccgc cgctgcggcc agacgctcgg tacagcctgg gctgacagca
     2941 aactgcgcac cggcatcgcg ggcttcgaga aactgctcgg cgtggatcac cgtaccggcg
     3001 ccgatcaaca ggtccggcaa ctccttgcgc atggccgcca gcgcatccag tgcccgcggc
     3061 gtgcgcaagg tcacttccag cacgcggatg ccgccagcgt gcagggcacg ggccaagtcc
     3121 accgccaggc tgatgtcttc aatcaccagc accggcagca ccgggcgggc ctgctgcagc
     3181 accacatcca ttgtcaggct cataccttgc tccccgctct caagattcaa tcgtcgcgct
     3241 cattcttcaa acaggctgct ggcgccctgc tcagccgaac cgaccaggcg gcgctgcgcg
     3301 gcgaacattt ccagaccata gcccaattcc agctcggcac tggcactggc caatggccgc
     3361 gcagccagtt gctgctcgct caattccacc tgcagcagcc cctgctgcgc atccagcacc
     3421 agccagtcgc cgtcctgcaa acgcgccaga gcgccgccag cggctgcctc cggggtcaag
     3481 tgcaaggcgg tggaaacctg cccggaagca ccggacatgc gcccatcggt aatcagtgcg
     3541 acacgctggc cggcttgctg cagattggcc agcagaggca tcagtttgtg cagctcgggc
     3601 atgccattgg cttgtgggcc ttggaagcgc accaccagca ccaggtcacc ttgcaactcg
     3661 ccggcgctgt aggccgcctg cacagctgct tccgaatcga agatgcgcgc ctgggcgcgg
     3721 atctgccaga acgccgggtc caccgccgag gttttcagaa tcgcgcggcc cagattgccc
     3781 gctagcagca ctaacccgcc ctcctcggca aacggctcgg ccacgtgccg gacaatatcc
     3841 ggggccgggc tctgcgctgg caggtcgcgc caggccagac gctcgccatc cagccagggt
     3901 tcgcgggtgt aggcgcgtaa accatgcccg gcagcggtgg cgacatcgtt atgcagcagg
     3961 ccagcaccga gcagctcacg cagcagccag gccggtccac cggcagcttg aaactgattc
     4021 acatccgccg cgccattggg ataaacccgc gccaacaatg gcaccaccct ggacagtgcg
     4081 gcgaaatcat cccagctcaa ttcataaccc gctgcccggg cgatggccgg caggtgcagg
     4141 gtgtggttgg tcgagccgcc gctggcgagc agtgccacca cggcgttgat cagcgcccgc
     4201 gcatcaatct gctggccgac cggcaagtaa cgctcaccct ggcggctgtt atgcgccacc
     4261 aggcgcgccg cttcatcggt catggcatcg cgcagcggcg tgtaggggtg aataaaggca
     4321 ctgcccggca catgcaggcc catggcctcc atcagcaact gattggtatt ggccgtcccg
     4381 tagaaggtgc aggtgccggc ctggtggtag gcggccaact cggcggccag cagttcgtca
     4441 cgactggctt cgccactggc ataacgctgg cggactgcgg ctttttcctt gttcggcaga
     4501 ccggattgca tggggcctgc cggtacgaac acggccggca gatggccaaa gtgcaaggcg
     4561 ccaatcagca gacctgggac gatcttgtcg catacgccca ggtacagcgc gccgtcgaag
     4621 atgccatggg tcagaccgat ggctgtggcc tgggcaatca ggtcccggga gaacagcgac
     4681 agctgcatgc caccctcgcc ctgggtaata ccatcgcaca tggccggcac cccggcggcg
     4741 aactgcgagg tcgcgcccgc cttggccagc gccactttca ggcgctcggg atagtccctg
     4801 agcggcgcat gggccgagag caggtcgttg taactggaaa tgatcgcgac atgcggcgag
     4861 ccgccctgct tcatgatcag ccgtgcatca tcgggctgtg cggccagggc atgggccagg
     4921 ttggaacagc tcagagcctt gcctgtggca cgcgtagccg ccagggccag gcgctgcaaa
     4981 tagcgctcgc ggcgcttggc cgagcgtttt tccagtgcgg cggtaacctg ctcaacaacc
     5041 gggtgcaaca taacggttcc ctttatcggc gaggtacaag gcttgcagtg taacggctta
     5101 gcacctgctt ttaccgctgc agagtcgcta tgcagtggac cttaatatca gctcagggtg
     5161 cccagtagat atgtaccggc aacggactgc gccgcaacca ggcgtacaga ggcaactcac
     5221 gcgccgaggg ctgatcggcc agcacggcat caatcaaggc gcgcttgcgc tcgccgaacg
     5281 ccagcaagcc gagccactgg ccgcgctgca acatggccag gttcaacgac agacgctcgc
     5341 gcggctcctg cggtgccagg gcgggtagag ctgcaggctc ggcatcagga tcaagcgccg
     5401 ccagcagccc tggcatagcg ggaaacaacg aagcgatatg gccgtcctcg cccataccta
     5461 gcagcaccgc gctaaacggc gaccactgca tcaactgctc gccccagcta agcgccgcct
     5521 gcgccaacgc ctcgccctgg cgcggatcga ggcaattggc ctgcggcagc ccttgatgta
     5581 acaactgcca gttgctctcg gccgcctgcg ctggcaccca gcgctcatca ctgggactga
     5641 ggtcaatgcg ctgactgtcc agctgctgcg ccagcaaggg caacaaggct tgcggactgc
     5701 tgccgccggg caggatcagc ccggcgcggg gctgctgcgc cagcgtggcc tggatctgcg
     5761 ctaacagatc ggaggccagc gcctgcaggc aagcatcacg ctgagcatgg caatgcaaca
     5821 aattttggct cataaacaac cccggctaag ctatcggtat gtcataaggc cgcgcaataa
     5881 tgcacctttg tcgtgcccac caacatcctt gccatggaat gcctgttttg atcgtgttgc
     5941 tgcgcgtact tcttctactg ttgctgccgg caatgacgca ggccaccagc ctgcctctgc
     6001 aagatagcga ctcacccttg ttgcctggcc cagccatgca ggtctggaca gacccagaag
     6061 gcctggcggg catccatagc gtgcgccaac tgcctgagtc tgcctggcaa ccggtggccc
     6121 gccgcgatgc cagtttcggc tacagcaccc acgcctactg gctgcgtttt accctgcaca
     6181 acccggcgga tgaggcgatc aactgggtat tgctgatcgg caacccgctg ctcgacttcc
     6241 tggatgccta cggcctggat ggcgaacggg tgtatcgcgc cggcgatcaa cgccccttcg
     6301 atgaacgctg ggtcaatcac cgccaactgg tattgccatt cagcttggca gctggggaag
     6361 aacgcgagct gatcctgcgt atgcagacca acggctcggc caacctcagc gccagcctga
     6421 tgagcgccca ggccttcaat cagcacgaac aacgcatgct gctattacag ggcctgtttt
     6481 tcggcgccct gctggtgatg atgatctaca acctgtcgat ctttatcatc acccgcgacc
     6541 gcaactacct gtggtacagc ctgtttgtcg ccagtttcag cctctaccag ttcatccagc
     6601 tgggttttgc cctgcaatgg ttctggcccg aatcactggc ctggcagcag atgagtttcc
     6661 cgttcagctc agcgctggca accctgttcg gtgtgctatt cgcccatggc gtactcgacc
     6721 tcaagaacca gcacccggcc tacagctgga tctgccgtgg cctgattggc agtagcctgc
     6781 tggtgatggt gctggcgctg gttgcgccct atctcgtggc actgatcggc agtttcgtgc
     6841 tgctgctggt ctgcgcgatt tttggctgca tcttcaccgt gctgcgctgg cgcgacggct
     6901 acccgccggc caaactgttc gccctcggct ggtcagccct gattatcgcc agcctgttca
     6961 gcgtgctcag cggcaccgga ctgatcgcca attcgctact gaccctgcac gcccagcaga
     7021 tcggcagcct gcttgagctg gtaattttct cgattgccct cggctcacgc attcgtctgg
     7081 cgcaacacgc ccagcaactg gctcaggaac agctatacgc caacgagcac caactgcgcc
     7141 tggaacaggc caagagcctg gatcttcagc aacagatcaa cgaaggcctg gaagctcgcg
     7201 tggaagagcg caccgcctcc ctgcaaaaag ccctgcagga gctttccacc gccaaccgcc
     7261 agttggccga actcagccgt cgcgacagcc tgaccgggct gttcaaccgc caggtgctca
     7321 acgaagaact ggagcgcatg ctcgcccagg ccaaacgcag ccagcagcca atcgccatgc
     7381 tgatgatgga cctggaccac ttcaagaagg tcaacgacca gcacggccac ctggttggcg
     7441 acgcctgcct acaacacgct gccctgcgta tgcagcagcg tatgcgcagt aacgatctgc
     7501 tggtgcgctt cggcggcgaa gagtttgccg ccgtgctctg cgacaccaat caatccggag
     7561 ccatggacct ggccgagcaa ctgcgtgcag acctggccag ccacccctgc atgcaccaag
     7621 gcctgagcat cgacctgagc ctgagcatcg gcgtctatgc gctgattccg gatatcggca
     7681 gcgaccgcga acagttgcta cgccacgccg accaggcgct gtatcgggcc aaggccgccg
     7741 ggcgcaataa ggttatcggt tacgaagcca gcgctttggg cgatgtctga ctcaacgact
     7801 tcaacagttc cagcaccgcc tgcaccaccc tcggatcagc caatacgcgc tgatgtccgc
     7861 cgctttccag gcgtaacagg cggctgtcga accagctctg atgaatcgcc tccgcttcgc
     7921 tgaatggcac cattggatcg tccaccgcat gcacgaccaa tccggggaaa tccaactggt
     7981 agtgcgcgct atcaagctgc ccagcgggaa tcccggcttt ctcttcgacc agccgcacaa
     8041 agtgcgcgcg cgcctgacgc ggcagcccca tatagcgaga gaagcgccgc agcatggtca
     8101 gaatccggct gggtgcggca atgctgacca acgcatcagt acgcaagccg agctgggtgg
     8161 ccaacaaggc actggcgcca cccatggaat gaccaatcac cgctttgaga ggcggcagtt
     8221 cgctggcagc ctccagcaag gcacgggcaa acagcaccac attggcttca cgcccgggcg
     8281 aacggccgtg ggccggggca tccaatgcca ccacgccgta gccggcattg accaaggcct
     8341 tgatcagctc ggcgaactgc gtcggccgcc cttcccagcc atgcatcaac aacacggccg
     8401 gcccactgcc ccagcgcagc gctgacagac cgaagcgcag ggtgatgcgc tcggcctgcg
     8461 ccagcagcgg caactcccag tcccgcggcg gcaaggtgtg cggcgtcata aactcccggc
     8521 gcagcttgct ggccagggca tacggcgcca gcagaccaat cgatgcgttg aaaccacgaa
     8581 cccagctcat gctattcatt gccataccct cagacaattg ccgatttagc ggcacgcaag
     8641 actcgatcag acaactcacc ggggcccagc gcgcgtgcca gcgccagccc tccgaccatc
     8701 aaggcgatat cggccagcgc ctggtcagcc tcttcaggac tgcccgccag ttgcgccgtc
     8761 atcagctcga tatgttcagc cagcacgccg cgaaaaccgt ccggcatacg cgccagctcg
     8821 cctaccgagc tgggcaacgg gcagccctgc tcgctggcat cgcggtgctt acgcgataga
     8881 taaaaggccg ccagcagcgt ccggcgctcg tgagcaggca gcgccggatc aaactgtgcg
     8941 acccgctccc gacgctgaca gagcatctgc tggaacacct ccagcatcag cgcatccttg
     9001 ctctcgaaat gcgcatagaa gccgcctacc gtcaggcccg cagcgcccat cacctcgccc
     9061 acgctgggtt cagccggacc acgctgcaac aacgccgcac tggcagcggc caggatgcgg
     9121 tcgcgggtat gcgccttctt ctcgctcatg tcagcctccg taatattaca accgaaatat
     9181 tataggcgta atacgattaa gcaagcgcca agggcaaccg ctgatcggct ggagcaataa
     9241 tgttcagtag gtagaaaaaa ttgcctggag caatttttaa cgtcgcgtag cgacggcccg
     9301 gagggtggcc gccagggatg gcaggccata aaaacaaaag ggccattcac taatgaatga
     9361 cccttcaaaa tcccgcagag cgggcaaaaa tggcgtcccc taggggactc gaacccctgt
     9421 taccgccgtg aaagggcggt gtcctaggcc actagacgaa ggggacgaaa
//
LOCUS       DASRUM010000029        46554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1185758, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000029 DASRUM010000000
VERSION     DASRUM010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 46554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46554
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185758"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VFF22_02190"
     CDS             <1..532
                     /locus_tag="VFF22_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HZX15664.1"
                     /translation="LAEHLAIDTVIWLPDGLFNDETDGHVDNFCCYVRPGEVLLAWTD
                     DATNPNYPRCQAAMRVLESVRDAKGRQLIVHKMPIPGPLYASDEECAGVDLVAGTQER
                     DPSIRLAGSYVNFLIVNGGIIAPKFDDPKDAEAEAILRRLFPQHRVVMVPGREILLGG
                     GNIHCITQQQPAPQIA"
     gene            614..2260
                     /gene="mgtE"
                     /locus_tag="VFF22_02195"
     CDS             614..2260
                     /gene="mgtE"
                     /locus_tag="VFF22_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HZX15665.1"
                     /translation="MKPTPAAPNDQQPQHNRTESEAITQALGSGKNKRVRKLLARMHP
                     GKIAALLEMLDTRQRQSLWQQIESSLEGRILPHLSLELIAQLAGDANDPLPSRPQGER
                     HGASVNHLEAVRQALALGKLKRVGKLLQRIHPAKVAGLLEALPPVERRAAWSMLETER
                     TGKVLTYLHDEIRGALALELDLEDLIASAEQLELDDRVDLIQTLPGELGSKLLHASNA
                     AQRQQLESMLSYPENSAGGLMNADPILVRADVKASTVLRYLRLLEKLPPQTDMLMVVD
                     RKGHYQGALRLSELVTADLGQRVSDLMHHDIAGIAVTTSSSEVARLFQDHDLLSAPVV
                     DEHNRLLGRITVDDVIDLIRENSDRALMHMAGLDDEADIFAPVLVSARRRALWLGINL
                     VTAFGAAWVIGLFQATLEQLVALAVLMPIVASMGGIAGSQTLTLVIRGLALGQVQKGN
                     IRILLNRELGISILNGILWSVVIALLAVAWFGNWGIGAVLGAAILLNLLCATLSGWGI
                     PLVLHRMGIDPALAGSVILTTVTDVIGFFAFLGLATLLLL"
     gene            complement(2528..2719)
                     /locus_tag="VFF22_02200"
     CDS             complement(2528..2719)
                     /locus_tag="VFF22_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HZX15666.1"
                     /translation="MKANVGTLDRSLRIAAGLILIGLSLSGVIGLWGLIGIVPLATGL
                     FRFCPVYPLLGISTCKTKP"
     gene            complement(2740..3156)
                     /locus_tag="VFF22_02205"
     CDS             complement(2740..3156)
                     /locus_tag="VFF22_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HZX15667.1"
                     /translation="MRKLLAFLAGVIFGIGLLSAGMANPAKVLGFLDLAGAWDPSLAL
                     VMAAAIGVAFLPFSWARWQRSSLLGAPMQLPVKRELDRRLIGGSLLFGIGWGIAGICP
                     GPAVAILLTGRWQVLLFVAAMLVGMVVFAALERQRG"
     gene            complement(3159..3590)
                     /locus_tag="VFF22_02210"
     CDS             complement(3159..3590)
                     /locus_tag="VFF22_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HZX15668.1"
                     /translation="MSIDWYNFTPWSALAGGALIGLAASLFVVINGRVAGISGLLGSL
                     LERGEGRGEKALFLLGVLLAPLLWQLFSSLPEIQIDNGWLALTVAGLLVGIGTRYGAG
                     CTSGHGVCGLSRLSPRSLAATLTFMAAGFATVFVLRHLLGG"
     gene            complement(3590..3913)
                     /locus_tag="VFF22_02215"
     CDS             complement(3590..3913)
                     /locus_tag="VFF22_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZX15669.1"
                     /translation="MTPEHPPIDIQQLRANADAAGQLLKALSNPDRLLLLCQLSQGER
                     NVSELEVLLGIQQPTLSQQLAVLRREGLVATRREGKQIYYCISSQAALAVINTLYQLF
                     CAGDH"
     gene            complement(4088..5341)
                     /locus_tag="VFF22_02220"
     CDS             complement(4088..5341)
                     /locus_tag="VFF22_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015802 - basic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family porin"
                     /protein_id="HZX15670.1"
                     /translation="MRKTSLALAVAASTLGLSLSQVAHADFIGDSKASIEARNFYYNS
                     DSREDAGQSKQEEWAQGFLLRMESGYTEGTVGFGVDALATFGFKLDSGDGTAGSGLLN
                     PDLSGGSQDNYGGLGLTAKAKVSESVLKVGALQLKNQAVGSSDSRLLPQVFRGANLVS
                     KDIDGLTLDVGYLDEVNNRNSTDYQDMRLNTFTNSGITVSGANQANQFIFAGGNYKLT
                     DNLTGGYYYSNLEDLYKQHSFNLVHVLPIADKQSFKSDLRYARSTDDGSSNVDNKAFG
                     AMFTYSLSGHSLGLGYQKMSGDTGFAYIGDSVDPFLVNYVQVGNFAAKDETSWQARYD
                     FNFASIGIPGLTFMTRYLTGDNIDRGAGSEGKEWERNMDIAYVVQEGTFKNLGIKWRN
                     ATMRNNFSRDIDQNRLIVSYTLPLL"
     gene            complement(5819..6790)
                     /locus_tag="VFF22_02225"
     CDS             complement(5819..6790)
                     /locus_tag="VFF22_02225"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HZX15671.1"
                     /translation="MDEISREQLEQEELTLALLHTRAEVARLQEREQLFSTLLGSVNA
                     VLWAFDWDDQRMIYVSPAYERIFGRSAALLLGGFEEWRNSIYPDDLDYAAESFAQVLE
                     TGAVEAREYRIIRADGQLRWLSDKCFVSRESSPGKPHIIVGIAEDITEKKQLEGELHR
                     LATTDVLTQSSNRRHFFECAQREFEQAREYGSPLAFLLLDLDDFKQINDRYGHQMGDQ
                     VLQRLAYSGVATLRRGDLFGRIGGEEFAALFPRCEPALAEQIAERLQREVRRLSFNHE
                     GTTFGVTLSQGLTSLRPEDTSLDALYTRADAAMYRAKRQGKNQVVVD"
     gene            complement(6956..8143)
                     /gene="desA"
                     /locus_tag="VFF22_02230"
     CDS             complement(6956..8143)
                     /gene="desA"
                     /locus_tag="VFF22_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-9 fatty acid desaturase DesA"
                     /protein_id="HZX15672.1"
                     /translation="MWYNGFLDLSVWQLIAVTLLMTHVTIVSVTVYLHRYSAHRALEL
                     HPALKHFFRFWLWLTTAQNTREWTAIHRKHHAKCETVDDPHSPVIKGLSTVLRTGAEL
                     YAEEAKNPETLRIYGKNCPEDWVERNLYSRFPMAGISLMAVIDLALFGAAGITIWAIQ
                     MMWIPVWAAGVINGLGHAVGYRNFECRDAATNLVPWGILIGGEELHNNHHTYPNSAKL
                     SVKKWEFDMGWFWIKLFSLLGLAKVQRVAPIAHRVEGKRSLDMDTAMAILNNRFQIMA
                     QYRKLVIAPLVQQELAKADASVRHHFHRAKRLLSREPSLLDEGHQARIQRMLEQSQAL
                     QVIYEKRLALQQIWVKTSANGHDMLEAIKHWVSDAEASGIQSLREFAEQLKTYSLRPA
                     VAA"
     gene            8311..10599
                     /locus_tag="VFF22_02235"
     CDS             8311..10599
                     /locus_tag="VFF22_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003160943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZX15673.1"
                     /translation="MNAPQGAWRLTLCYMLAAGLWVLFSDGLLVGLGLSLAQLERAQL
                     LKGLGFVLLTAGLLYTVLRAHRQQHSLVHQALRSSEKRLHQALEAANDGMWDWDLQTQ
                     KLYFSPGYAALLGIQPDALGDNPQQWLQRLHADDRASFDRNLEQRLAARNPEPYEVNY
                     RLLHQDGSYRWIQSRGRLLLDEHGQAERLIGTASDITQRRKDEDSLRQAAAVFDATQE
                     GVLVTDAQQHIVHCNPAFTRITGYSQAEILGQSPSMLKSGRHDKNFYDSLWHALQNRG
                     AWSGEVWNRRKSGEIYPQWQCIRVIHDELGEISHYVAVFSDISALKRSQRELDYLAHH
                     DPLSNLPNRLLFTERVERALERAKIDEQGGAVLLIDLDHFKNINESLGHNIGDLLLKD
                     VGERLGQQLGKRMTLARLGGDEFGLLDEGCSSAEQAAVMAQRLQACLTQSFRINQQEL
                     FITASIGISLYPFEAADVGQVLRNADSALFKAKSSGRESYAFYSQELTEHARQRVELA
                     SALRHALDHQELRVYYQPLICLRNQEMIGVEALVRWQHPQRGLVAPGEFIPIAEETGL
                     IGAIDSWVLEQSCRQMVAWQIAGYKLKFVAVNVSSRLFSRGELDVRVAEVLRNTGLDP
                     AHLELEVTESAVMDNPDAALALLQRLRALGIRLAIDDFGTGYSSLARLKRLPVHKLKL
                     DQSFVSGLPHDQDDIAITRAVIALGHSMGLKVLAEGIEQPEQVEMLQQLGCDHAQGYF
                     FGRPQPAEQLTTHLALSAATTN"
     gene            10610..11743
                     /locus_tag="VFF22_02240"
     CDS             10610..11743
                     /locus_tag="VFF22_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HZX15674.1"
                     /translation="MRVMILEDDSWIADLLKQIVLSLRPGSQVVCLDSVSAALREWQQ
                     RPTEMVITDWNLPDGPGTLLLEQVRRDNSNTPLVVITGRSDRASVMAVRALKINAFIS
                     KPFQAAHVAASLETLLPPDNGEQQRPISDTQNDLLDHLRGLPSSALQLPLKAHVRDRL
                     LQSFKGEQIDLRELASEWQHDPALSARLLTVANSSAYNQAGKSCSSLLEALQKLGATN
                     SLNLALGLALRQCSALDNPLLRMLAQAHLDSTELLIERSTNLARQCALNPAPFHSAAL
                     LHRMGELCVLYLAQQWQDSGQGFSEAQVMQALDQFSGPFAISIKAQWQLPMHLRELIG
                     ACYNLPANNVKRENILMRLAACELNSAADDPELARLCRLAGLT"
     gene            11774..16234
                     /locus_tag="VFF22_02245"
     CDS             11774..16234
                     /locus_tag="VFF22_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012020086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZX15675.1"
                     /translation="MNKQIKPSAITWIVGLGSLGLAIFFSVLGGFALNERETLWNLQI
                     AKQHELQSLTLRNSQQELQQQAQLLAETIAADAWLVELVRQANALQSNQPLDTQSLNA
                     IRNQLYTRLAPRWRNLQGIRPFALYIHLAPKAEVLLRVHEPQWFADFPAAQRPMLLDS
                     LQQSKASVGLAVDGEKLSMRAIVPLQIDNLEGRLNVGALEVGLDVLGNLQQLDRELEA
                     GVALLLKRSIQAPNDSGEALRATVEGSDYWHLAGFSQEQVQRWQAQQRLPPPEAGNTF
                     RLLDDQGHTYLLNQVRLNDYQPNTGLVSPVVALTWRDITALYNLHQRDKRWLVVKWLL
                     AWLAAEALLLALLQATRHSSQQVMHRHHAELQHKHQQTEESRQLLALITQTQAAYINA
                     DNQREAFDTLLGRILEVSASQFGFVGEILHDDADAPFLRTFAISNIAWDAQSRAFYAA
                     HAPQGLEFRNLDTLFGQVIRSGQALISNDPSNHPQSAGLPPGHPPLLAFAGLPIHANG
                     ELLGMLGLANRAGGYDEQFAAQLQPLLTTLGQLLEALRRDTQRAQTQQHMQRQQAALR
                     ALNEIAALPKLSSHEQLRQALQLGAEFYGMPVGIISQINADTYQVMVQVSPSNTLQDG
                     QCFPLGDTYCSITLRSKDVLAIAAMGQSEHAAHPCYPLFALESYIGTSIWVAGQRFGT
                     LCFASGEARNAPFDDADEEFMRLFARWVGATLERMRHEEQTREARRFLQAVLDSATGV
                     SIITTNTDGLITLFNSGAERLLGYRSAEVIGQHSPALFHLSEEIQARGQQLSRQLGSE
                     VSGFAVFTRLPELGEPETRQWTYRRKNGEQRIVNLTVSAMRDAEGVITGYLGIASDIN
                     DLHQTTRALQKSESRFRGLVSSLPGAVYRCRNDESWSMSYLSEEIQVLSGYLASDFIN
                     NSVRSFSSVVHPDDLPITFRAVAAIERQESFELTYRLLHADGHSVWVREKGRGEYDSH
                     GELQWISGFIWDISDRKAVEDQLRLSQQRFSSAFSTAPQGMALVSLQGQWLEVNEVLC
                     EMLGYSREELLRSNFQQITHPDDLDADLLHIEELLAGEVSSYQMEKRYLDSQGRIIWV
                     LLSVSLVRDSDDAPVHFVVQIQDFNERIAAEMAIREREDYLRTLLDNVLDAIITIDQQ
                     GYIETFNHAAERIFGYSHLEVSGHRVTLLMPEPHRSMRARYLSHYLKTGISQVLGKEI
                     ELTAMRSNGETFAIELAISQISHQGERRFIAVIRDIEERKRIERMKNEFVSTVSHELR
                     TPLTAIAGSLGLVNGGALGAVPASMQQMLQIAQDNSQRLNLLINDLLDMDKLVAGKML
                     FELHDEALQPLLEQAIKENQPYADHYQVQLQLQGPPTQAHVRVDRLRLAQVLANLLSN
                     AAKFSPQGQTVDVRVLQQGESIRVSVTDHGPGVPENFQARIFSKFSQADATDTRQKGG
                     TGLGLAISKEIIERMGGQIGFNSNPGHGATFWLELTTLALEASPEDNRNA"
     gene            16227..16613
                     /locus_tag="VFF22_02250"
     CDS             16227..16613
                     /locus_tag="VFF22_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012020089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX15676.1"
                     /translation="MPELQRILHVEDDASIQAVAKLALEAVGGFQVLSCSSGQDALDQ
                     LLGFAPDFILLDVMMPDMDGPQTLARIAQLIDIHQVPVAFMTAKVQPAEIAHYRSLGA
                     RDVIIKPFDPMQLAAQVRKIWSLAHE"
     gene            16606..18258
                     /locus_tag="VFF22_02255"
     CDS             16606..18258
                     /locus_tag="VFF22_02255"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZX15677.1"
                     /translation="MSNGKSAADELQQHLQQLSNAFAVRLQVELPALAQNAERLLHAA
                     DPQEQLQHLHSLRDQLHKLAGAAGTFGFSSLGQRARELEQEADQWLETLQKEQQSLQD
                     FSRSLQQLARQQFQAERSQEPATRTVKPPRPNSSARRIYILEDQLSIGENMRLTLNNF
                     GYHAEHFSRISELDAALQQQLPDALIVDVNLPDESLTGLEYAAGLQQRLGEPLPLLVI
                     TTQNDFATYLEAVRVGAMGFFTKPVDIPQLENRLERCFAQQQGEPYRVLIIDDDRELA
                     SRFSLILRGANMLVEMLHEPAEIFARMRSFNPEVVLLDVSMPDCTGPELAQIIRMNDD
                     WLRVPIIYLSAETDINRQMNALIKAGDDFVTKPISDNALVAAVFSRAQRARLLSNALS
                     RDSLTGLLKHADIKEQAAVELERALRSGKPASVAMLDIDFFKKVNDSYGHAAGDNVIR
                     ALANLLRQRLRRIDSLGRYGGEEFLVVLPDCPSDQALRILDEIRQRFAELHFIAGGQQ
                     FSVTLSAGISSTEDAPTGADDLLDKADRALYAAKHNGRNQVR"
     gene            18615..19904
                     /locus_tag="VFF22_02260"
     CDS             18615..19904
                     /locus_tag="VFF22_02260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZX15678.1"
                     /translation="MFKQSAQHIGTYYAGTYPGQIPLRPSLQEALQCDVLIIGGGFSG
                     LHTALRLALAGKKVVLLEASRLAWAASGRNGGQALPGWSCDMPPFEKALGLERARRLW
                     DSMVWATEEMRELPERHNFDIDYRSGAIYTAVLPRRVRQLQDSLEEAEQKYGYTQLSF
                     IPKEQLPEWIASPRYLAALHDSGAAHLNPLKLAQGLADAIEKAGGKIFEQSKALSYQE
                     TADSYVARTEQGEVRSDVLVLACNAYIDKLDPQLSKRLLPVGSYQVATAPLDPELARS
                     LFPRGTCAIDNQFVPDYFRVTPDNRLLFGGGCTYLGGIPQDVPAATRPYLERVFPQLR
                     GVQIDYGWGGHIDCTMHRTPDVGRSGQKYWLQGFSGHGILPTLAAARAVSDAILGGAD
                     LLELYQGLSNPRFPGGDLLAAPIEAVGKAWYRLRDVI"
     gene            19916..20473
                     /locus_tag="VFF22_02265"
     CDS             19916..20473
                     /locus_tag="VFF22_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012271450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZX15679.1"
                     /translation="MDMQEEVEGLAILIRDLRKHKNLTLGELAARIDRSVGFLSQVER
                     GLSRPTVADLTAISETLGVPTTYFYSLSKPRDVPWVTRPGERRTLYYAGGITDVLASP
                     TMAGGFSMLESHLQPGATSGDGHLNDSSEQGGFVLEGELTIWYGDTAEPVTLGPNDSF
                     QLPPHAQFRYANLSDSPTRVLWVFT"
     gene            20498..21844
                     /locus_tag="VFF22_02270"
     CDS             20498..21844
                     /locus_tag="VFF22_02270"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013972149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HZX15680.1"
                     /translation="MTTAFPDLLSEVRAFRSANPEVRYVDLISLDIPGHFYGKRYPID
                     MLEKVAAGSPLKIPQNCVLLGVQGGLHPIGDYCFNDGDPDAPRRLIPGTLKPVRWESQ
                     PLGQMLISSDGTDAPIEFEPREVLARVLKRLAARGIRPVVAFELEFYLFDRKLQDGLP
                     QFPRDPLCDDADDQPNMHIERLSRFSDVLHEIVDAANEQGVDANVITAELGPGQFEIN
                     FGHCDDGLRAADWAALFCRSTRGVALKHGQRTSFMSKPYLHAPGSGMHVHVSLYDAAG
                     NNLLAADEQRPLRHAVAGCLELLPHCMPIFAANHNAFRRYGAMVNAASRASWGFEDRD
                     ACIRIPESDGKNLRIEHRLAGADANPYLVLAAILTGMEHGLDAGQEPIAPLNENRQSG
                     IDFPQDMLGAVSAMRDHPVVNQGLGSEFVMVYCENKRQDHLAFMQEVSAREYRWFL"
     gene            complement(21969..23312)
                     /gene="chrA"
                     /locus_tag="VFF22_02275"
     CDS             complement(21969..23312)
                     /gene="chrA"
                     /locus_tag="VFF22_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017245821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HZX15681.1"
                     /translation="MSESSAQPAPPTVSLGEAFWFWLKLGLISFGGPAGQIAILHQEL
                     VEKRRWLSERRFLHALNYCMLLPGPEAQQLATYIGWLMHRTWGGVIAGVLFVLPSLFI
                     LIGLSWLYIAFGDVPLVAGIFYGIKPAVTAIVMQAAWRIGSRALKNNWLWGIAGASFV
                     AIFAFNLPFPLIVLGAALLGYLGGRLLPQQFSLGGGHAQQGKSYGPALIDDDNPPLPH
                     TRFRASRLALLLAVGALLWLLPMSLLTVLYGWEGTLTQMAWFFTKAALLTFGGAYAVL
                     PYVYQGAIGHYGWLTPTQMIDGLALGETTPGPLIMVVAFVAFVGAYLQPVFGGDSGFV
                     SGALAAALVTWFTFLPSFLFILAGGPLVESTHGELKFTAPLTAITAAVVGVILNLALF
                     FAYHVLWPQGFGGTFDWPSALLAMVAALALLRFKRGVMEVLLVSALAGLAVHLLR"
     gene            23456..24361
                     /gene="pbpG"
                     /locus_tag="VFF22_02280"
     CDS             23456..24361
                     /gene="pbpG"
                     /locus_tag="VFF22_02280"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014850953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HZX15682.1"
                     /translation="MKIRHSIVSLLLICSCVVVASNTQAAPAKQQDLAAGSALLVDLQ
                     TNQVLYERNADSVVPIASVTKLMTAMVTLDAKLPLDQQLPIMIRDTHDMRGVFSRVRV
                     GSELSRRDMLLLALMASENRAAASLAHHYPGGHAAFVAAMNAKARALGMKNSRFVEPT
                     GLSEHNVSNARDLVLLLKAARQYPLISQFSTTSEKTQAFRKPNYTLGFRNTNNLVRKP
                     GWSVQLSKTGYTGEAGRCLVMNTVMNNRPVAFVVLDAFGKYTHMADANRLKRWLETGK
                     VTAVPPAAISYRQQKLAQRQASAAQ"
     gene            complement(24415..26391)
                     /locus_tag="VFF22_02285"
     CDS             complement(24415..26391)
                     /locus_tag="VFF22_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018927516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX15683.1"
                     /translation="MTSPRSRIAWQLATALALVLVLLISVSTLFALRSLSEANLVTRQ
                     EHLGSEARLLADQLSTFHDSLRESTQRLAGLFERRFSGGLSVQGAERIAVADMQAPAL
                     YLGSALLNNNFGEVDEFRQLTAGVATVFVRDGDEFVRISTSLTKPDGTRAIGTALDHQ
                     HPAYRRLLSGEGYVGRALLFERFYMTQYTPVRDSSGRVIAVLFVGFDYTDAQNMQFEN
                     LKRFRIGATGSLALLDDQGKWLVPPAGAREPEHIAGKLADVLAEPGQGAFWSDGVEDF
                     FSVAMPFSGGPWQVLASMPREEIQAVTWHVGSQLAIGSLVTLLLAVAAVIWLLRRKLR
                     PLGELVQQAQALGAGDLSARLNVTSQDEIGELARNFNQMGEALSTMVARIRGAAHNVS
                     SRAQALSGLASGAYEGIEQQSGEISSMAGAVEEFSATSLNIADNMRNTERLASDNAQQ
                     TRIGRSSMAEASSALEQIASSLNDTSVVVNTLGQRSQEIGGIVSVITSIADQTNLLAL
                     NAAIEAARAGEQGRGFAVVADEVRSLASRTREATNEISAMIGSIQGETNSAISTMEQG
                     KSLMQDGLQRNAKVAAALEQIAEQSSAAGEQFTAITTATSEQSSTATLLSSNLQSIAL
                     ANSEQREVVANLAQTARELDQLAEELRQEVGRFQ"
     gene            complement(26635..28305)
                     /locus_tag="VFF22_02290"
     CDS             complement(26635..28305)
                     /locus_tag="VFF22_02290"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HZX15684.1"
                     /translation="MSVFVEPLRLLLLAETPGWAQLLRERLSALGSGYALITAPSWEA
                     ASALFDKPSNAVLLTTAKYLPASGRCSLPIVLLLDAEPEAEPEGVCDWLVRDQLTVEV
                     LRRCLRYVRERGSLNDTLHRLAEQDALTGIANRQGFQTLLAARLAEYEGRGLTLGHLD
                     LDNFRHANDALGYQGGDSLILQVVARIKAQLHAGDQVARLGSDEFALLLDSRRDPQRA
                     ERLAAQITEALGEPYWIDGESLLIGCSLGLAHSRAEGSADPLMWHAHIAMQQAKSQQG
                     CTFHIYDERINRSARNQADLESELRRALRRDELELHYQPRLCLQTGRIVGLEALVRWR
                     HSEHGLLAPNEFVPLAEESGLIVPLGYWVISRALRDMQWLRGRGLPALHMAINLSFRQ
                     FQDSQLLPTLSRLIEERGVDAQWLEFELTETAVMRRSDQVQQTMLALGRLGVRFSLDD
                     FGTGFSSFVHLNNLPITLLKIDKSFVGGMGERAENRQLVRAMINLAHNLNLQVVGEGV
                     ENAEQLALLRQYGCDQVQGYLISKALPLAELARFLVFGMRQPLLGLNG"
     gene            28572..30581
                     /gene="rep"
                     /locus_tag="VFF22_02295"
     CDS             28572..30581
                     /gene="rep"
                     /locus_tag="VFF22_02295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HZX15685.1"
                     /translation="MSRLNPRQQEAVNYVGGPLLVLAGAGSGKTSVITRKIAHLVQNC
                     GIQARHIVAMTFTNKAAREMKERVGTLLKGPEARGLTVSTFHNLGMNIIRKEYAALGY
                     KPGFSIFDEGDIKALLSDIMQKEYSGDDGADEIKNYIGSWKNDLILPEQALAESRNPK
                     EQTAAIVYLHYQRTLKAYNAVDFDDLILMPVKLFQDNPDILEKWQNRIRYLLVDEYQD
                     TNASQYLLVKLLVGMRNQFTVVGDDDQSIYAWRGARPENLMLLKDDYPSLKVVMLEQN
                     YRSTSRILKCANILIANNPHAFEKQLWSEMGMGDEIRVIRCKNEDAECERVALEILTE
                     HLRTQRPYSDYAILYRGNYQAKLMELKLQHHQIPYRLSGGTSFFARQEVKDLMSYFRL
                     LVNPDDDNAFLRVINVPRREIGSTTLEKLGNYATSRKISMYAAAGEIGLGEQLDARYT
                     ERLGRFTKWMDRVRQECAQNDPIAAIRSMVMDIDYENWLRQNASNDKVADARMGNVWF
                     LVEALKNTLERDEDGEMTIEDAIGKLVLRDMLERQQEEEDGAEGVQMMTLHASKGLEY
                     PSVYILGVEEEILPHRSSIEADTVEEERRLAYVGITRAKRNLTMTFAAKRKQYGEIID
                     CSPSRFLDELPPEDLVWEGLEDAPPEVKAATGNSALANMRAMLKK"
     gene            30713..31285
                     /locus_tag="VFF22_02300"
     CDS             30713..31285
                     /locus_tag="VFF22_02300"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HZX15686.1"
                     /translation="MEALKQKIRDEGTVLSEQVLKVDAFLNHQIDPKLMQQIGHEFAR
                     RFAGLGITKIVTIEASGIAPAVMAGLELGVPVIFARKYQSLTLTDNLYISKVFSFTKQ
                     TESTLAISAKHLNAKDHVLLIDDFLANGHAARALIDLIGQAGASIAGIGVVIEKSFQR
                     GRAELDAQGYRVESLARIGSLSDGQVSFLD"
     gene            complement(31298..33427)
                     /locus_tag="VFF22_02305"
     CDS             complement(31298..33427)
                     /locus_tag="VFF22_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HZX15687.1"
                     /translation="MPKTCTIEQAVDHVLSAIDGPIHLGLPLGLGKPNQWVNALYARI
                     KQLPQRQLTIYTALCLGRPRAAAGLPSRLLEPFVERIYGDYPELDFLADLQRDTLPAN
                     VRVEQFFLQPGSLLDSATTQQNYISSNYSHVARDLNDKGLNLIAQLVAHDPQRPEHFS
                     LSCNPDITLDLLPLLEQRRAAGETILCLAQVHSALPYMVGDAEVSRRSFDLHLLEEDR
                     STLFSTPNMPVTLQDHCIGLHASALVRDGGTLQIGIGAMGDALTAALLARQADNQAYR
                     ALLNELRCTIHWAPEIAKDGGLQPFSEGLYGCSEMFVNGLLALADAGIVRRPVYPELH
                     LQQLASGGALDAQGMPRSVQALLDAGLAPQLDAQALQGLQASGLLAESIRWADGHLLM
                     PDGQQLLSDLRGARTQQVLQQHLGRARGGVVIHGGFFLGPQAFYQRLCELSAVQLGRF
                     AMTGICFINELHNQEALKRLQRRDARFINTAFTMTLLGAGVADQLEDGRVLSGVGGQY
                     NFVAQGHALQGARSILLLRSWRQAGGKVSSNIVWDYGHATIPRHLRDMVVTEYGIADL
                     RGKTDSQVIEALLNISDSRFQSGLIEQAQRAGKLPKDFRLNPRFAGNLPERLQAVQNC
                     HPGLFAEYPLGCDFDNEERDLLRALSWLKSKLRFACALELAVAIWRAPNPGKYDAHLQ
                     RMALAPPRGLRQRVQRRLLLAGLRASS"
     gene            complement(33541..33963)
                     /locus_tag="VFF22_02310"
     CDS             complement(33541..33963)
                     /locus_tag="VFF22_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012274807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZX15688.1"
                     /translation="MNLIKKLLVAPATVLALWAVTAQATTDEAIAERLKPVGQVCVMG
                     EECKGVGAVAVAAGGGARTADDIITKHCGACHTPGILGAPKIGDAAAWKARADAEGGL
                     DGILAKAISGINAMPPKGTCADCSDDELRGAIQKMSGL"
     gene            complement(34074..34622)
                     /locus_tag="VFF22_02315"
     CDS             complement(34074..34622)
                     /locus_tag="VFF22_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZX15689.1"
                     /translation="MDVGVRLQSIRKLKGLSQRELAKRAGVTNSTISMIEKNSVSPSI
                     SSLKKVLAGIPMSLVEFFSLDVEQDSQAQVVYRAAELTDICSGAITMKLVGKAHPTRA
                     IAFLDETYPGGTDTGDEMYAHEGEEAGMLVEGKLELTVGDEVFILEPGDSYYFESSKP
                     HRFRNPFEQPARLISATTPANF"
     gene            complement(34725..35798)
                     /gene="alr"
                     /locus_tag="VFF22_02320"
     CDS             complement(34725..35798)
                     /gene="alr"
                     /locus_tag="VFF22_02320"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZX15690.1"
                     /translation="MRPARALIDLQALRHNYQLAREVSGARALAVIKADAYGHGAVRC
                     AQALQEQADGFAVACIEEALQLREAGIRGPILLLEGFFEADELPLIEQHELWCVVHSL
                     WQLEAIERSAVRGPLTVWLKMDSGMHRVGLHPADYQAAYQRLLASGKVAKIVLMSHFA
                     RADELDCPRSDEQLAVFQQARQGIVAEVSLRNSPAVLGWPNVPSDWVRPGIMLYGATP
                     FEQAQAVAARLQPVMTLESKIISVRELPAGEPVGYGARFFSERPTRVGVVAMGYADGY
                     PRHAPTGTPVAVDGQLTRLIGRVSMDMLTVDLTDLPQAGLGSRVELWGKQVLASDVAA
                     QAGSIPYQLFCNLRRAPLLYLGS"
     gene            complement(35963..36313)
                     /locus_tag="VFF22_02325"
     CDS             complement(35963..36313)
                     /locus_tag="VFF22_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZX15691.1"
                     /translation="MSVQRLHTESRYSEIVIHNGTVYLAGQLADDYSGDIVQQTRENL
                     ANTDRMLAEAGSDKSKILSVTIYLKDMERDYAGLNQVWDAWVAPGAAPARACVEAKMY
                     KPEVLVEMMIVAAL"
     gene            complement(36288..37586)
                     /gene="dadA"
                     /locus_tag="VFF22_02330"
     CDS             complement(36288..37586)
                     /gene="dadA"
                     /locus_tag="VFF22_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HZX15692.1"
                     /translation="MRVLVLGSGVIGTASAYYLARQGFEVVVVDRQHAPAMETSFANA
                     GQVSPGYASPWAAPGVPLKAIKWLLQKHAPLAIKATADIDQYLWMAQMLRNCTASRYA
                     VNKERMVRLSEYSRDCLDELRAETGIAYEGRSLGTTQLFRTQAQLDNAAKDIAVLQQS
                     GVPYEVLDRAGIARVEPALAAVTDKLAGALRLPNDQTGDCRLFTTKLAEMAKALGVEF
                     RFGQDIQRIDAVGDRVNGVWIDGKLETADRYVLALGSFSPQLLKPLGIKAPVYPLKGY
                     SLTVPITNVAMAPTSTILDETYKVAITRFDNRIRVGGMAEIAGFDLSLNPRRRATLEM
                     ITADLYPQGGDLSQAEFWTGLRPATPDGTPIVGATGLRNLFLNTGHGTLGWTMACGSG
                     RLLADLMAKKRPQISAEGLDISRYSRSQESHKHVSTAPAH"
     gene            37742..38230
                     /gene="dadR"
                     /locus_tag="VFF22_02335"
     CDS             37742..38230
                     /gene="dadR"
                     /locus_tag="VFF22_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DadR"
                     /protein_id="HZX15693.1"
                     /translation="MRTQHQSRRELDKIDRNILRILQEDGRISFTELGERVGLSTTPC
                     TERVRRLEREGIIMGYHARLNPQQLKASLLVFVEISLDYKSGDTFEEFRRAVLKLPHV
                     LECHLVSGDFDYLVKARINEMASYRKLLGDILLKLPHVRESKSYIVMEEVKESLSLPI
                     AD"
     gene            complement(38227..38580)
                     /locus_tag="VFF22_02340"
     CDS             complement(38227..38580)
                     /locus_tag="VFF22_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZX15694.1"
                     /translation="MSCTSRKIDQLRLQIPSFACIPGCHDCCGPVTASSEEMSRLPVK
                     SAAEHDAALAEYNCVHLGPQGCTVYDQRPLICRLFGTTPSLPCPRGQGPEQMIEPAVA
                     KQVHQLIATTRQVLV"
     gene            38755..40068
                     /locus_tag="VFF22_02345"
     CDS             38755..40068
                     /locus_tag="VFF22_02345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014340850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZX15695.1"
                     /translation="MNARVHQPVHNSQHANSYYATSANRQLGYPALGGELRADVCIVG
                     GGFSGLNTAIELAQKGLSVVLLEAHKIGWGASGRNGGQLIRGVGHGVEQFESVIGAQG
                     VRELKLMGLEAVEIVRQRIEQFNIDCDLTWGYCDLANKPSHLADFAEDMAELKQLGYR
                     HEMRLLQPEQMHEVVGSNRYVGGMIDMGSGHLHPLNLALGEAAAAQSLGVQLFENSAV
                     TRIDYGSEVKVHTAQGVVRAATLVLGCNAYLNGLNSNLGGKVLPAGSYVIATEPLSEA
                     EARAIIPQNMALCDQRVALDYYRLSADRRLLFGGACHYSGRDPANIAGYMRPKMLEVF
                     PHLKDVKIDYQWGGMIGIGANRLPQIGRLKEQPNVYYAQAYSGHGVNATHLAGKLLGE
                     AIAGQASSGFDLFAKVPHMTFPGGKHLRSPLLALGMLWHRMKELV"
     gene            complement(40112..40357)
                     /locus_tag="VFF22_02350"
     CDS             complement(40112..40357)
                     /locus_tag="VFF22_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008030560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HZX15696.1"
                     /translation="MNGLSDVRLTMKSEELQQESTGEHLYAQAGAVKPAVSRWNAFWL
                     RLTTRRALLTLTDEQLKDVGLNREQARREAFLPFWKL"
     gene            40540..42006
                     /locus_tag="VFF22_02355"
     CDS             40540..42006
                     /locus_tag="VFF22_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZX15697.1"
                     /translation="MTLYINLAELLGTRIEQGLYRPGDRLPSVRALSLEHGVSLSTVQ
                     QAYRHLEDQGLATPRPKSGYFVPQARHQPALPMVSRMAQRPVDVSQWDQVLELISRPP
                     GQEDLLQLGRGRPDIDSPTLKPLLRSLSRLSRRQDANSLTYGCIYGHLGMREQISRLM
                     LDSGCSLSPQEIIVTTGCHEALSAAIRAICEPGDIVAVDSPSFHGVMQALKGFGMKAL
                     ELPTDPITGISLEALELALEQWPIKAIQLTPTCNNPQGYIMPDANKRALLALAQRYDV
                     AIIEDDVYGDLAYSYPRPRTIKSYDEDGRVLLCSSFSKTLAPGLRIGWIAPGRYLDRV
                     LHMKYMGTGSTAQLPQLAITEYLQTGHYEPHLRRMRAQYQRGRDLMTDWISRYFPAGT
                     RVSRPQGSFMLWLELPTGFDSQRLNRELLQHAIQIAPGNIFSAAGKYRNCLRINYAGK
                     ATPELEQAIRQVGECVAALLSEQQIAETAADAMLTSWR"
     gene            complement(41990..43138)
                     /locus_tag="VFF22_02360"
     CDS             complement(41990..43138)
                     /locus_tag="VFF22_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX15698.1"
                     /translation="MNWMTYFAVSAAVVIVGLSLGVTLPLVSFRLEAWGYGPFAIGVM
                     AAMPAVGVLLGARLTGHLAGWFGTPQTLRLCLLASAASVALLSVMPSYPLWLLLRLLI
                     GISLTVVFVLGESWINQLVEDRLRGRLVALYGTGFALSQLCGPLLLTLLGTESDRGFW
                     FSAALLVGGSLLLLGRDGAPKVDAQSASGRGILAFCRTSPAIAWAVVLFAAFEAMVLT
                     LLPVYLVREGFAQQLALVMVSAVVVGDAALQLPIGWMADRVPRQTLFRCCGVVLLLSS
                     LSIPLLLHTPLIWVVLVLFGASAGGLYTLSLILVGQRYRDDALVRANSHIALLWGAGC
                     LLGPLSTGAASQWVSGHALPILMAVGAAVFVWLAFQRGAFSEVASARM"
     gene            complement(43282..44775)
                     /locus_tag="VFF22_02365"
     CDS             complement(43282..44775)
                     /locus_tag="VFF22_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZX15699.1"
                     /translation="MTSLTRADWEQRAKNLKIEGRAFVHGEYRAAVSGATFECISPVD
                     GRLLGLVASCDLADAELAVSDARATFESGVWSRMAPAQRKRVMIRFADLLDQHAEELA
                     LLETLDMGKPISDALSVDVPAASRAIRWSGEAVDKIYDEVAATPHDELGLVTREPVGV
                     VAAIVPWNFPMIMTCWKLGPALATGNSVIVKPSEKSPLTALRMAQLALDAGIPAGVLN
                     VLPGYGHTVGKALALHMDVDTLVFTGSTKIAKQLMIYAGESNMKRVWLEAGGKSANIV
                     FADAPDLKAAAEAAAAAICFNQGEMCTAGSRLLVERSIKDTFMPMVLEAMQGWKAGHA
                     LDPDSKVGALVDAGHLNAVLGYIDAGHKDQAKLLCGGKRTLEETGGQYVEPTIFDDAH
                     NAMRIAQEEIFGPVLTVIPFDSTEEAIKIANDSIYGLAAAIWTSNLSKAHLAAKALRV
                     GSMWVNQYDGGDMTAPFGGFKQSGNGRDKSLHAFDKYTEIKATWIKL"
     gene            45027..45392
                     /locus_tag="VFF22_02370"
     CDS             45027..45392
                     /locus_tag="VFF22_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZX15700.1"
                     /translation="MNIEKIVDFATVTTTPEHYRPAAEKVLKGDPEQSVRNHYGSPCG
                     QFNAGIWEGAVGHWTINYTEHEYCEILQGVSVLRDKDGNAKTLRAGDRFVIPAGFSGT
                     WEVLEPCRKVYVIFEQASN"
     gene            complement(45544..45699)
                     /gene="rpmG"
                     /locus_tag="VFF22_02375"
     CDS             complement(45544..45699)
                     /gene="rpmG"
                     /locus_tag="VFF22_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HZX15701.1"
                     /translation="MRELIRLISSAGTGHFYTTDKNKRTTPDKIEIKKFDPVVRKHVI
                     YKEGKIK"
     gene            complement(45710..45946)
                     /gene="rpmB"
                     /locus_tag="VFF22_02380"
     CDS             complement(45710..45946)
                     /gene="rpmB"
                     /locus_tag="VFF22_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HZX15702.1"
                     /translation="MSTVYQVTGKGPVTGNNISHANNKTRRRFLPNLQHHRFWVESEN
                     RFVRLRVSAKGMRCIDKRGIDVVLSELRARGEKV"
     gene            complement(46220..>46554)
                     /gene="radC"
                     /locus_tag="VFF22_02385"
     CDS             complement(46220..>46554)
                     /gene="radC"
                     /locus_tag="VFF22_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HZX15703.1"
                     /translation="ARLRHEPHEVFACLFLDAKHRVLAFEVLFHGSIDGASVYPRQVV
                     KRALAHNAAALILTHNHPSGVAEPSQADRVLTRRLKEALELVDVRVLDHFIVGDGEPL
                     SMAEYGWL"
BASE COUNT         9624 a        15049 c        13682 g         8199 t
ORIGIN      
        1 cctggccgag catctggcca tcgacacagt gatctggctg ccggacggtc tgttcaacga
       61 cgagaccgac ggccacgtcg acaacttctg ctgctacgtg cgccccggtg aagtgctgct
      121 ggcctggacc gacgatgcca cgaaccccaa ctacccgcgc tgccaggccg ccatgcgcgt
      181 gctggaaagt gtgcgggatg ccaaaggccg tcagctgatc gtgcacaaga tgccgattcc
      241 cggcccgctg tatgccagcg atgaagaatg cgccggggta gacctggtgg ccggtactca
      301 ggagcgcgat ccatcgatcc gcctggctgg ctcttacgtt aatttcctga tcgtcaacgg
      361 cggcatcatc gcgcccaaat tcgacgaccc caaggacgcc gaagccgaag caatcctgcg
      421 tcgcctgttc ccgcagcacc gcgtggtgat ggtgccgggt agggagattc tcctgggcgg
      481 cggcaatatc cactgcatca cccagcagca gcctgcgccg caaatagcct gatatgaccc
      541 ctgccggtgt ttggccaagc caacaatcac cggctggggg caactgatcg cctgcggatg
      601 ataggctgag cccatgaagc caactcccgc agcacccaac gaccaacagc cccagcacaa
      661 ccgcactgaa agcgaggcca tcacccaggc actcggcagc ggtaaaaaca aacgcgtgcg
      721 taaactgctg gcgcgcatgc accccggcaa gattgccgca ctgctggaaa tgctcgacac
      781 gcgccagcgc cagagcctct ggcagcaaat cgagtcgagc ctggaaggcc gtattctgcc
      841 ccatctcagc ctggagctga ttgctcagtt ggccggcgac gcgaatgacc cactgccgtc
      901 gcgcccgcaa ggcgagcgcc atggggccag cgtcaaccac ctggaagccg tgcgccaggc
      961 tctggccctg ggcaaactca agcgggttgg caaactcctg cagcgcattc acccagccaa
     1021 ggtcgccggt ctgctcgaag ctctgccccc cgtcgagcgg cgcgcagcct ggagcatgct
     1081 cgagaccgag cgcaccggca aagtgctgac ctacctgcac gacgaaatcc gtggcgcact
     1141 ggcgctggaa ctggacctgg aagacctgat cgcctcggcc gagcagctgg agctggatga
     1201 tcgcgtcgac cttatccaga ccctgcccgg cgaactcggc agcaaactgc tgcatgccag
     1261 caacgcggcg cagcgccaac aactcgagtc gatgctgagt taccccgaga actcggccgg
     1321 cggcctgatg aacgccgacc cgatcctggt ccgcgccgac gtcaaggcca gcacagtgct
     1381 gcgttacctg cgtctactgg aaaagctgcc gccgcaaacc gacatgctga tggtggtcga
     1441 ccgcaagggc cattaccagg gcgccttgcg cctgagcgaa ctggtcacgg ctgatctcgg
     1501 ccagcgcgta agcgatttaa tgcatcacga catcgccggt atcgccgtca ccaccagcag
     1561 cagcgaagtc gcccggttgt ttcaggacca cgacctgctc tccgcacccg tggtcgatga
     1621 gcacaaccgg ctgctcggac gcatcaccgt ggacgatgtg atcgacctga ttcgcgaaaa
     1681 ctccgaccgc gccctgatgc acatggccgg cctcgacgac gaggctgaca tcttcgcgcc
     1741 agtgctggtc agcgcccgac gccgcgcctt gtggctgggc atcaatctgg tcacggcctt
     1801 cggcgccgcc tgggtcattg gcctgtttca ggcaaccctg gagcaattgg tagccctcgc
     1861 cgtactgatg ccaattgtgg ccagcatggg cggtatcgcc ggcagccaaa ccctgaccct
     1921 ggtcattcgc ggcctggcgc tgggtcaggt gcagaagggc aacattcgca tcctgctcaa
     1981 ccgcgaactg ggtatttcca tactcaacgg tattctctgg tcggtggtaa tagccctgct
     2041 ggcggtcgcg tggtttggca actgggggat tggcgcagtg ctcggcgcgg ccatcctgct
     2101 caacctgctc tgcgccacat tgtccggctg gggcattcca ctagtgctgc accgcatggg
     2161 gattgacccg gcgctggccg gtagcgtgat tctgacgacg gtgaccgacg ttatcggctt
     2221 ctttgccttt ctaggcctgg cgaccctgtt gctgctttaa tgctctcggc taacactgct
     2281 tgcggcaggt gctgtagcaa cggggccaga cccaaaactg ccgccaggca aacaccccaa
     2341 ccagcggcgt ggccacattg ctctgcaccg ccagagccga gcgggaaaaa ctcgccgcca
     2401 ccgtaccgac cacccgcacg ccataggcta gctgtaggct gagctggcag agtcgaaaaa
     2461 agcctgaatg agcggggaca tggcagctcc agccgaccgg cccagaggcc cggtcagcac
     2521 tccagcgtta gggcttggtc ttgcaggtgc tgatacccag cagcgggtat accggacaga
     2581 agcgaaacag accggtggcc agcggcacga tgcctatcag gccccatagg ccgatcacac
     2641 cactcaggct caagccaatc agaatcagac ccgcggcaat gcgcaggcta cggtcaaggg
     2701 tgccaacatt tgctttcata gtgatctccc gatcatcaat tagccgcgct ggcgttccag
     2761 cgcagcaaac acaaccattc ccaccagcat ggccgccaca aacagcagca cctgccagcg
     2821 cccggtgagc agaatcgcca cggccggccc cggacaaata cccgcgatgc cccagccaat
     2881 gccgaacagc agactgccgc cgatcaaccg gcggtccagc tcacgcttga ccggcaactg
     2941 catcggcgca ccgagcaaac tgctgcgctg ccaccgcgcc caactgaacg gcagaaacgc
     3001 cacgccaatc gccgccgcca tcaccagtgc caacgaagga tcccaagcgc ccgccaaatc
     3061 gagaaaaccc agtaccttgg ccggattggc catacctgcg ctaagcaggc cgatgccgaa
     3121 gatcacaccg gcgagaaacg ccaacagttt gcgcatattc agccccccag cagatgacgc
     3181 aggacaaaca ccgtggcgaa cccggcggcc ataaacgtca gggttgcggc cagagaacgt
     3241 ggcgacaacc gcgacaggcc gcagacccca tgcccgctgg tgcaaccggc gccatagcgg
     3301 gtgccgatac ccaccagcaa accggcaacc gtcagcgcca accaaccgtt gtcgatctga
     3361 atctccggca ggctgctaaa cagctgccag agcagcggcg caagcagcac gccgagcaga
     3421 aaaagcgcct tctcgccacg gccttcacca cgctccagta ggctgccgag caggccgctg
     3481 ataccggcca cccggccatt tattacgacg aacaggctgg cagccagacc gatcagcgca
     3541 ccaccagcca gggcactcca gggcgtgaag ttgtaccaat cgatgctcat caatgatcac
     3601 cggcacagaa cagctgatac agggtgttaa tcactgccag tgcggcctgg ctgctgatgc
     3661 agtaatagat ctgcttgccc tcacggcggg tcgccaccaa cccttcacgg cgcagcaccg
     3721 ccaattgctg cgacagggtc ggctgctgaa taccgagcaa tacttccagc tcgctgacat
     3781 tgcgctcgcc ctgggagagc tggcacagca gcagcagtcg gtccgggttg ctcagggcct
     3841 tgagcaactg ccccgccgca tcggcattgg cgcgcagttg ctggatatcg attggcggat
     3901 gttcaggcgt catggcaagc gtctcattta agataaaaac aatctattct tttgtatatt
     3961 gaatgacaag ccccgccaac taccacacgc ataaacggcg agcataaaaa aacccggaac
     4021 agagtccggg tttcgtttgc tgctcaggct gggctggatg caacagctca gccagcaacc
     4081 accacaatca gagcagtggc agggtgtagc tgacgatcag acggttctgg tcgatgtcac
     4141 ggctgaagtt gttgcgcatg gttgcgttgc gccacttgat gccgaggttc ttgaaggtgc
     4201 cttcctgaac cacgtatgca atatccatat tgcgttccca ctctttgccc tctgaacctg
     4261 cgccgcgatc aatattgtcg ccagtcaggt agcgggtcat aaaggtcagg ccagggatac
     4321 caatggacgc gaagttaaag tcgtaacgag cctgccagga agtttcgtcc ttggctgcga
     4381 agttgccaac ctgcacgtag ttgaccagga aggggtcaac actgtcgccg atataggcaa
     4441 agccggtatc gccgctcatt ttctgatagc ccagacccaa gctatggcca ctgagggagt
     4501 aggtgaacat ggcaccgaag gccttgttgt ctacgttgct agagccatca tcggtcgagc
     4561 gggcgtagcg caggtcgctc ttgaacgact gtttgtcggc gatcggcagc acatgcacca
     4621 ggttgaagga gtgctgtttg tacagatctt ccaggttgct gtagtagtag ccgccggtca
     4681 ggttgtcggt cagtttgtaa ttgccgccgg caaagatgaa ctgattggcc tggttggcac
     4741 cgctgacagt gatgccgcta ttggtgaacg tattcaggcg catgtcctga tagtcggtgg
     4801 agttgcggtt gttgacctca tccaggtagc ccacgtcgag ggtcaagcca tcaatatcct
     4861 tggataccag gttggcccca cggaagacct gaggcagcag acggctatca ctggaaccaa
     4921 cagcctggtt cttcaactgc agagcgccaa ccttgagcac gctctcggac accttggctt
     4981 tggccgtcaa acccagaccg ccgtagttgt cctgggagcc gccggacaga tccgggttga
     5041 gcagaccgct acccgcagta ccatcgccag agtccagttt gaagccgaaa gtggccagcg
     5101 catcaacgcc gaaacctacc gtgccctcgg tatagcccga ctccatgcgc agcaggaaac
     5161 cctgggccca ctcttcctgc ttggactgac ctgcgtcctc gcgggaatcg ctgttgtaat
     5221 agaagttacg cgcttcgatg ctggctttgc tgtcgccaat aaaatcagca tgcgccactt
     5281 ggctcaggga caagcccagg gtgctggcag cgacggccag ggcaagggag gtctttctca
     5341 ttatgtgttc tccaggtgtg tgtgggttgc acatcggcgc gggtactgtc ttcagcctat
     5401 gaagcggcag gcgcgcaccg aattcgggaa gtattgcagg ccaggcaggc gggcaagtgc
     5461 cgcagccaga accggccgtg caatatcgat aatcaggtga gcgcgtgaag gcagtcagcg
     5521 cgaactgggg aatgaggaca tagcagcgga caagtgcaga tcatggcgca ggtctcttca
     5581 ttttttattg tcggccattg attctggccg ttatgcgctg agccttacgc agatactgtg
     5641 ccagctgccc gaatcggctc gccaggctgt ctattgcaag gcgtttcacg gtattcaaac
     5701 aagaacgaga gcatttccaa ctagaagata ggcggtttct tgcctatcgc aaacctagaa
     5761 aaggcgactt ttcgccaatc gacctcgtca cctgcacgcc agcagccagt gcgcgcggtc
     5821 aatccaccac aacctggttc ttaccttggc gtttggcgcg gtacatcgcg gcatcggcgc
     5881 gcgtatacaa ggcatccagg ctggtgtctt caggtcgcag gctggtcagg ccctggctta
     5941 gggtcacgcc gaaggttgtg ccctcatggt tgaagctcag ccggcgcacc tcacgctgca
     6001 ggcgctcggc aatctgctcg gccaacgccg gctcacagcg tggaaacagc gcggcaaact
     6061 cctcaccgcc gatgcgccca aataaatcac cacggcgcaa tgtggcgacg ccgctgtagg
     6121 ccaggcgctg tagcacctga tcacccatct gatgaccata gcggtcgtta atctgcttga
     6181 agtcgtcgag gtcgagcagc aaaaacgcca gcgggctgcc atattcgcgg gcttgctcga
     6241 actcgcgttg ggcgcattcg aagaagtgcc ggcggttgct gctctgggtt agcacatcgg
     6301 tggtggccag gcggtgtaac tcgccttcga gctgtttctt ctcggtgatg tcttcggcga
     6361 tgccgacgat gatatgcggc ttgcccggcg agctttcgcg gctgacaaag cacttgtcgc
     6421 taagccagcg cagctggccg tcggctcgga tgatgcggta ttcgcgagcc tcgaccgcgc
     6481 cggtttccag cacctgggcg aagctttcgg cagcgtaatc gagatcatcg gggtagatgc
     6541 tgttgcgcca ctcttcaaaa ccgcccagca gcaacgcggc ggagcggcca aagatccgct
     6601 cgtaggcggg actgacgtag atcatccgct gatcatccca atcaaacgcc cagagcaccg
     6661 cattgacgct gcccagcaag gtgctgaaca actgctcgcg ctcttgcagg cgcgccacct
     6721 cggcgcgggt atgcaacagc gcgagggtca actcttcctg ttcgagctgc tctctgctga
     6781 tttcgtccat aacaaccgcc attgcataga ggtaccgagc aaagacagcg gcaggcgcca
     6841 gaaattcaaa ccgatcagac tggagcgtgc gccaaattta gtctgaaaag ggactacttg
     6901 aatggtaggc cataaaaagc ccgccaatag gcgggcttag taaccaaacg gcgcgttatg
     6961 cggcgacagc agggcgcagc gaataggttt tcagctgctc agcgaactca cgcagcgact
     7021 gaataccgct ggcttcggca tcactgaccc agtgcttgat ggcttcaagc atgtcgtggc
     7081 cattggcgct ggtttttacc cagatctgct gcagggccag acgtttctcg tagatcacct
     7141 ggagcgcctg actttgctcc agcatgcgct ggatgcgcgc ctggtggcct tcgtcgagca
     7201 gacttggctc acgcgacagc aggcgcttgg cacggtgaaa atgatgacga accgaggcat
     7261 cggccttggc cagctcctgc tgcaccagcg gcgcgatgac cagcttgcgg tactgcgcca
     7321 tgatctggaa acggttgtta agaatggcca tggcggtgtc catgtccagg ctgcgcttgc
     7381 cttccacccg gtgagcgatg ggcgcaacac gctgcacctt ggccaggcca agcaggctga
     7441 acagcttgat ccagaaccag cccatatcga attcccactt cttcaccgat agcttggcgg
     7501 agttcgggta ggtgtggtgg ttgttatgca gctcttcgcc gccgatcagg atgccccaag
     7561 gcaccagatt ggtcgcggca tcgcggcatt cgaagttgcg atagccgacg gcatggccga
     7621 ggccgttgat cacgcctgct gcccagaccg gaatccacat catctggatc gcccagatgg
     7681 tgatgcccgc agcgccgaac agagcgaggt cgatcaccgc catcaggctg ataccggcca
     7741 tggggaagcg tgagtagagg ttgcgctcga cccagtcttc cgggcagttc ttgccgtaga
     7801 tgcgcagggt ttccggattt ttcgcctctt cggcgtacag ctcggcaccg gtacgcagta
     7861 cggtgctcag gcctttgatc accgggctgt gcgggtcatc gacggtttcg catttggcgt
     7921 ggtgtttgcg gtggatcgcg gtccattcgc gggtgttctg cgccgtggtc agccacagcc
     7981 agaaacggaa gaagtgcttg agcgccgggt gcaattcgag ggcgcggtgc gccgagtagc
     8041 ggtgcagata gaccgtgacg ctgacgatag tcacgtgcgt catcagcagg gtgaccgcga
     8101 tcaactgcca gaccgacagg tcaagaaaac cgttgtacca catggatcat gtgcctcgtg
     8161 cagaaggaaa aacgccgcta gttttagcac acgtcgacgg ccattatccc tgaccagggt
     8221 cagaaaacca gttggtcttt tgcataagaa tgttgccccc ttataaacac ctatactgcc
     8281 ggtcttagac aaaggactga cttatatccc atgaatgctc cccaaggcgc ttggcgcctg
     8341 actctctgct atatgctggc cgctggcctt tgggtgctat tcagcgacgg tttactggtc
     8401 ggcctgggcc tgtctctggc gcaactagaa cgcgcacaac tgctcaaggg gctgggcttt
     8461 gtgctgctca ctgcaggctt gctgtatacc gtgctgcgtg ctcatcgcca gcagcacagt
     8521 ctggttcacc aggccctgcg cagcagcgaa aagcgcctgc atcaggcgct ggaagccgcc
     8581 aatgacggca tgtgggactg ggacctgcag acccagaagc tgtatttctc ccccggctac
     8641 gccgccctgc tcggcattca gcctgatgca ttgggcgata acccgcagca gtggttgcaa
     8701 cgcctgcatg ccgatgaccg tgcgagtttc gaccgcaacc tggagcagcg cctggccgca
     8761 cgcaaccctg aaccctatga ggtcaactat cgcctgctgc accaggacgg cagctaccgc
     8821 tggattcaat cacgcggccg cctgctgctc gatgaacacg gccaggccga acgcctgatc
     8881 ggcaccgcca gcgacatcac ccaacggcgc aaggatgaag acagcctgcg ccaggccgct
     8941 gcggtattcg atgccaccca ggaaggcgtg ctggtcaccg atgcccagca gcatatcgtc
     9001 cactgcaacc cggcctttac ccgcatcact ggctatagcc aggcggagat cctcggccag
     9061 tcaccgagca tgctgaaatc cggacggcac gacaaaaact tctatgacag cctctggcat
     9121 gccctgcaaa accgcggcgc gtggagcggc gaggtgtgga accggcgcaa gagcggcgag
     9181 atttacccgc agtggcaatg tattcgggta atccacgatg aactgggcga gatcagccat
     9241 tacgtcgcgg tgttctccga tatcagtgca ctgaaacgct cgcaacgtga gctggattat
     9301 ctggcgcacc acgacccgct gagcaacctg cccaaccgcc tgctgtttac cgaacgcgtc
     9361 gagcgtgccc tcgaacgcgc aaaaatcgat gagcaaggcg gcgcggtgct gttgatcgac
     9421 ctcgaccatt tcaagaacat caacgaaagc ctcggccaca acattggtga tctgctgctc
     9481 aaggacgtgg gcgaacgtct cggccaacaa ctcggtaagc gcatgaccct ggcgcgcctg
     9541 ggcggtgacg agttcggcct gctcgatgaa ggctgcagca gcgccgaaca ggcggcagtc
     9601 atggcgcagc gtctacaggc atgcctgacc caatctttca ggatcaacca gcaagaattg
     9661 tttatcaccg ccagcatcgg catcagcctg taccccttcg aggccgctga tgttggccaa
     9721 gtgctgcgca atgccgactc ggcgctgttc aaggccaaga gcagcggccg tgagagctat
     9781 gccttctaca gccaggagct gaccgaacac gcgcgccagc gcgtcgaact ggctagcgcc
     9841 ctgcgccatg ccctggatca tcaggagctg cgcgtctatt accaaccgct gatctgcctg
     9901 cgcaaccagg agatgatcgg cgtcgaagcc ctggtgcgct ggcagcaccc gcaacgcgga
     9961 ctagtggccc ctggtgaatt tatcccgatt gccgaagaaa ccgggctgat tggggccatc
    10021 gattcctggg tgctggaaca gagttgccgg cagatggttg cctggcaaat cgccggatat
    10081 aagctgaaat ttgtcgcggt gaatgtttcc agccgcctgt ttagccgcgg cgagctggat
    10141 gtgcgggtgg ctgaggtgct gcgcaatacc ggcctcgatc ccgcgcattt ggagctggaa
    10201 gtcaccgaaa gcgcagtgat ggacaacccc gacgccgccc tggccttgct gcaacgcctg
    10261 cgcgctttgg gcattcgtct ggccattgat gatttcggca ccggctacag ctccctggcg
    10321 cgccttaagc gtctgccggt gcacaaactc aagctcgacc agagttttgt cagcggcctg
    10381 ccgcatgatc aggacgacat agcgatcacc cgcgcagtga tcgccctcgg ccacagcatg
    10441 gggctcaagg tactggctga gggcatcgag cagcccgagc aggtcgaaat gctccagcaa
    10501 ctcggctgcg accacgccca aggctacttt ttcggccgcc cgcaaccggc agaacagctg
    10561 accacccact tggcgctaag cgccgcaaca acgaactaag gcacaacgca tgcgggtcat
    10621 gattctggaa gacgacagct ggattgccga tctgctcaag cagatcgtcc tcagcctacg
    10681 tcccggcagc caggtggtgt gcctggacag cgtcagcgcc gccctgcgcg aatggcaaca
    10741 gcgccctact gaaatggtca tcactgactg gaatctaccg gacggccccg gcaccctgct
    10801 gctcgagcag gtgcgccgcg acaacagcaa cacgccgctg gtggtcatca ccgggcgttc
    10861 cgatcgcgcc agcgtaatgg ccgtgcgcgc cttgaagatc aacgccttta tcagcaaacc
    10921 gtttcaagcc gcgcatgtcg ccgccagcct ggaaaccctg ctccccccag acaacggcga
    10981 acagcaacgc ccgatcagtg atacgcagaa cgacctgctc gatcacctgc gcggcctgcc
    11041 cagcagcgcc ttgcaactac cactcaaggc gcatgtgcgt gatcgattgt tgcagagctt
    11101 caagggcgaa caaatcgatc tgcgcgaatt ggccagcgag tggcaacacg accccgcgct
    11161 cagcgcgcgt ctgctcactg tggccaacag cagtgcctat aaccaggcag gcaagtcctg
    11221 cagcagcctg ctcgaagcgc tgcagaagct gggcgcaacc aatagcctga acctggcctt
    11281 gggcctggcc ctgcgccaat gcagcgcgct ggataatccc ctgctgcgca tgcttgccca
    11341 ggcgcacctg gacagcaccg agctgctgat tgagcgcagc accaacctcg cccgccaatg
    11401 cgccctgaac cctgcgccct tccacagcgc ggcgctattg caccgcatgg gcgagctgtg
    11461 tgtgctgtac ctggcacagc aatggcaaga cagcggccag ggcttcagcg aagcccaggt
    11521 catgcaggcg cttgaccaat tcagcggtcc ctttgccatc agcatcaagg cacaatggca
    11581 attgccgatg cacctgcgcg agctgatcgg cgcgtgctac aacctgccgg ccaacaacgt
    11641 caaacgcgag aatatcctca tgcgcctggc cgcctgcgag ctcaacagcg cagccgatga
    11701 tcccgaactg gcgcgcctgt gccggctggc ggggctgacc tgatggcgtc cttggtgcgc
    11761 aggaccctga ttgatgaata agcagataaa gccctcggca attacctgga tcgtcggcct
    11821 cggcagcctg ggcttggcga tcttcttcag cgtgctcggc ggctttgccc tgaacgagcg
    11881 ggaaaccctg tggaacctgc aaatcgccaa gcagcatgaa ctgcaaagcc tgacgctgcg
    11941 caacagccag caggagttgc agcaacaggc gcagctgctg gccgaaacca tcgccgccga
    12001 cgcctggctg gtcgaactgg tgcgccaggc caatgcactg cagagcaatc aaccgctaga
    12061 tacgcagagc ctcaacgcca tccgcaacca gctctacacc cgcctcgccc cgcgctggcg
    12121 caacctgcaa ggcattcgcc cattcgccct gtatatccac ctggcaccta aggccgaagt
    12181 gctgctgcgg gtgcatgagc cgcagtggtt tgcagacttc cccgctgcgc aacggcccat
    12241 gctgctcgac agcctgcagc agtccaaggc cagcgtcggc ctggcggtcg atggcgaaaa
    12301 gctcagcatg cgcgccatcg tcccgctgca gatcgacaac cttgagggcc gactcaacgt
    12361 cggcgcgctg gaggtcggcc tcgatgtgct gggcaacctg caacagctgg accgcgagct
    12421 ggaggccggt gttgcactgc tgctcaagcg ctcgatacag gcacccaacg acagtggcga
    12481 ggcgctgcgc gccaccgttg aaggcagtga ttactggcac ctggccggtt tttcccagga
    12541 gcaggtgcag cgctggcagg cccagcagcg cctgccgccc cctgaggccg gcaacacctt
    12601 caggctgctc gacgatcaag gccacaccta cctgctcaac caggtgcgcc tgaacgacta
    12661 tcagcccaat actggcttgg tcagccccgt agtggctctg acctggcgcg atatcacggc
    12721 actgtacaac ctgcaccagc gcgacaaacg ctggctggtg gtcaagtggt tgctggcctg
    12781 gctcgccgcc gaagcactgc tgctggcctt gctgcaggcc acccgacaca gcagccagca
    12841 ggttatgcat cgccaccacg cagaactgca gcacaaacat cagcaaaccg aggaatcgcg
    12901 ccagctgctg gcgctaatca cccagacgca ggcggcctat atcaatgcag ataaccagcg
    12961 cgaggccttc gacaccctgc tcgggcggat tctcgaagtc agcgccagcc agttcggctt
    13021 tgtcggggaa atcctgcatg acgatgccga cgcgcccttt ctgcgcacct tcgccatcag
    13081 caatatcgcc tgggatgcac aaagccgcgc cttctacgcc gcccatgcgc cgcagggcct
    13141 ggaatttcgt aacctcgaca ccctgttcgg ccaggtgatc cgcagcggtc aggcgctgat
    13201 cagcaacgac ccaagcaatc acccgcaaag cgccggcttg ccgccagggc acccgccgct
    13261 actggcattt gccggcctgc ccattcacgc caacggtgag ttgcttggaa tgctcggcct
    13321 ggccaaccgc gcaggcggct acgacgagca gtttgccgcc cagctgcaac cgctgctcac
    13381 caccctcggc cagctgctcg aagccctgcg ccgcgatacc cagcgcgcgc aaacccagca
    13441 acacatgcag cgccagcaag cggccttgcg cgcgcttaac gaaattgccg cactgcccaa
    13501 gctgagcagc cacgaacagc tgcgccaggc cctgcagctg ggcgccgagt tctatggcat
    13561 gccggtaggc atcatcagcc agataaatgc ggacacctat caggtcatgg tgcaggtatc
    13621 gccgtccaat accctgcagg acggccagtg cttccctctc ggagatacct attgcagcat
    13681 caccctgcgc agcaaggatg tgctggccat tgccgccatg ggccagagcg aacatgccgc
    13741 gcacccctgc tacccattgt tcgccctgga aagctatatc ggcaccagca tctgggtcgc
    13801 cggtcaacgc ttcggtaccc tttgcttcgc ctcaggcgag gcgcgtaatg cgccatttga
    13861 tgatgccgac gaagagttta tgcgcctgtt cgcccgctgg gtcggcgcca ccctggagcg
    13921 catgcgccat gaagagcaga cccgcgaggc acggcgcttc ctccaggcag tgctcgattc
    13981 ggccaccggg gtcagcatca ttaccaccaa caccgacggc ctgatcaccc tgttcaactc
    14041 cggcgccgaa cgcctgctgg gttatcgcag tgcagaggtc atcggccagc acagccccgc
    14101 actgttccac ctgagcgagg aaatccaggc gcgtggccag cagcttagcc gccagcttgg
    14161 cagtgaagtc agcgggtttg cggtgttcac tcgactccct gaactgggcg agccggaaac
    14221 ccgccagtgg acctaccggc gcaaaaacgg cgagcagcgc atcgtcaacc tcaccgtcag
    14281 cgccatgcgc gatgccgagg gggtgatcac tggctacctg ggcatcgcca gcgacatcaa
    14341 cgacctgcac cagaccaccc gcgccctgca aaagagcgag agtcgcttcc gcggcctggt
    14401 cagcagtttg ccgggcgcgg tgtatcgctg ccgcaacgat gaaagctggt cgatgagcta
    14461 cctcagtgaa gaaattcagg tactcagtgg ctacctggcc agcgacttta tcaacaacag
    14521 cgtacgcagc ttttccagcg tggtgcatcc cgacgacctg ccgatcacct tccgcgcagt
    14581 ggccgccatc gagcgtcagg agtccttcga gctgacctac cgcctgctgc acgccgacgg
    14641 ccacagcgtc tgggtgcgag aaaaaggccg cggcgaatac gacagccacg gcgagctgca
    14701 gtggatttcc gggtttatct gggacatcag cgaccgcaag gccgtggaag atcagctgcg
    14761 tctgagccag cagcgcttca gcagcgcctt cagcacggca ccgcagggca tggcactggt
    14821 gtcgttgcaa ggacagtggc tggaggtcaa tgaagtgctc tgcgaaatgc tcggctacag
    14881 ccgcgaagag ctgctgcgca gcaacttcca gcagatcacc caccccgacg acctggatgc
    14941 cgatctgctg catatcgaag agctgctggc tggcgaggtc agctcctatc agatggagaa
    15001 acgctacctg gatagccaag ggcggatcat ctgggtgctg ctgagcgtgt ccctggtgcg
    15061 cgacagcgac gatgcgccgg tacactttgt ggtgcagatt caggacttca acgaacgtat
    15121 cgccgccgaa atggccatcc gcgagcgtga ggattacctg cgtaccctgc tcgacaacgt
    15181 gcttgatgca attatcacca tcgaccagca gggctatatc gaaaccttca accacgccgc
    15241 cgaacgcatc ttcggctata gccacctgga ggtctccgga caccgggtaa cactgctgat
    15301 gcccgagcca caccgctcaa tgcgcgcccg ctacttaagc cattacctga aaaccggaat
    15361 cagccaggta ctcggcaagg aaatcgagct gaccgccatg cgcagtaatg gcgagacctt
    15421 cgccatcgag ctggccatat cgcagatcag ccaccagggc gagcggcgct ttatcgcggt
    15481 aatccgcgat atcgaggagc gcaaacgcat cgagcgaatg aagaatgaat tcgtctccac
    15541 cgtcagccac gaactgcgca ccccgctgac cgcgattgcc ggctccctcg gcctggtcaa
    15601 tggcggcgcc ctaggcgccg tgccggccag catgcagcag atgctgcaga tcgcccagga
    15661 caacagccag cgcctcaacc tgctgatcaa tgacctgctc gacatggaca aactggtggc
    15721 cggcaagatg ctcttcgagc tgcatgacga agcgctgcaa ccgctgctgg aacaagccat
    15781 caaagaaaac cagccgtacg ccgaccacta ccaggtgcaa ttgcagttgc aaggcccacc
    15841 cacccaggcc cacgtgcgcg ttgacaggct gcgcctggcc caggtgctgg ccaacctgct
    15901 gtccaacgca gcgaagttct cgccccaggg gcagaccgtc gatgttcgcg tgctgcagca
    15961 gggcgaaagc atccgcgtca gcgtgaccga ccacggcccc ggtgttccgg aaaacttcca
    16021 ggcacgaatt ttcagcaaat tctcccaggc cgacgccacc gacacgcggc aaaaaggcgg
    16081 caccggccta ggcctggcga tcagcaagga aataatcgag cgcatgggcg ggcagatcgg
    16141 tttcaactca aaccccggcc atggcgcgac tttctggctc gaactgacta ccctggcctt
    16201 agaggccagc cctgaggaca accggaatgc ctgagctaca gcgcattttg cacgtggaag
    16261 acgacgcgtc gatccaggcc gtggccaagc tggcgctgga ggcggttggc ggttttcagg
    16321 tgctaagttg ctcatccggc caggacgccc tggatcagct gctgggcttt gccccggact
    16381 ttatcctgct ggatgtgatg atgccggata tggacggccc gcaaaccctg gcacgcatcg
    16441 cccagctgat cgacattcat caggttccgg tggcctttat gaccgccaag gtgcaaccgg
    16501 cggaaatcgc ccattaccgc agcctcggcg cccgcgatgt gatcatcaaa cccttcgacc
    16561 cgatgcagct ggccgcacag gtgcgcaaaa tctggagcct ggcccatgag taacggcaag
    16621 agcgctgccg atgaactgca gcaacacctg cagcaactga gcaatgcctt cgccgtgcgc
    16681 ctgcaggtgg agttgccggc cctcgcgcag aatgccgaac gcctgctgca cgccgccgac
    16741 ccgcaagagc agctgcagca tctgcatagc ctgcgcgacc agctgcacaa gctggctggc
    16801 gccgccggca ctttcggttt cagcagcctt ggccagcgcg cccgcgagct ggagcaggaa
    16861 gccgatcagt ggctggaaac cctgcagaag gaacagcaaa gcctgcagga tttcagccgc
    16921 agcctgcagc agctggcgcg ccagcagttc caggccgaac gtagccagga gcccgccact
    16981 cgcacggtca agccgccacg gccgaacagc agcgcgcggc gcatttatat cctcgaagac
    17041 cagctgtcga ttggcgagaa catgcgcctg acgctgaaca acttcggcta ccacgccgag
    17101 cacttcagcc gcatcagcga gctcgacgcc gccttgcagc agcaactgcc cgatgcgctg
    17161 atcgtcgacg tcaacctgcc cgacgagagc cttaccggcc tggaatatgc cgccggcctg
    17221 caacaacgcc tgggcgagcc actgccgctg ctggtgatca ccacccagaa cgacttcgcc
    17281 acctacctgg aagcggtgcg cgtcggcgcc atgggctttt tcaccaaacc ggtggacatc
    17341 ccgcagctgg aaaatcgtct ggagcgctgc ttcgcccagc aacagggcga accgtaccgg
    17401 gtgctgatca tcgacgacga ccgcgagctg gccagccgct tcagcctgat tctgcgcggc
    17461 gccaatatgc tggtggaaat gcttcacgag ccggcggaga tcttcgcccg catgcgcagc
    17521 ttcaaccccg aggtggtgct gctggatgtc agcatgcccg attgcacagg ccctgaactg
    17581 gcacagatca tccgcatgaa cgatgactgg ctgcgcgtgc cgatcatcta cctttcggcg
    17641 gaaaccgaca tcaaccggca gatgaacgcc ctgatcaagg ccggcgatga cttcgtcacc
    17701 aaaccgattt ccgacaacgc cctggtcgct gccgtgttct cccgcgccca gcgcgcacgc
    17761 ctgctgagca acgcgctatc gcgcgacagc ctcaccgggc tgctcaaaca cgccgacatc
    17821 aaggagcagg cggcggtcga gctggagcgc gcgctgcgca gcggcaaacc ggccagcgtg
    17881 gcgatgctgg atatcgactt tttcaaaaag gtcaacgaca gctacggcca tgccgctggc
    17941 gacaacgtga tccgcgccct ggccaacctg ctgcgccaac gcctgcgacg catcgacagc
    18001 ctgggccgtt acggcggcga agaattcctg gtggtgctgc ccgattgtcc atcggaccag
    18061 gcgctgcgga ttctcgatga gattcgccag cgctttgccg aactgcactt tattgctggc
    18121 ggccagcagt tctcggtaac cctgagcgcc ggcatttcct ccactgaaga tgcgccaacg
    18181 ggtgctgacg acctgctgga taaggccgac cgcgcgctgt acgccgccaa gcacaacggg
    18241 cgcaatcagg tgcgctagtc ggcgcctgtt aggccgggtg tcacctaggt ggctccaccc
    18301 atccacggtc ttctgaagac cggcatgtag cccggataag cgcagcgcaa tccggggccc
    18361 cgctatcccg gattgcgcca aggcttatcc gggctacagg ggctcgtaag agcggtgcag
    18421 atgcccagga ctatgaccgc gaataggcat attggtcagc ttacagcgct tgccgaggct
    18481 atgcgggatc tagggatcgc ctgcgtctgg cccatcggtt tctagagtcc agaagccttc
    18541 gcgcttggct ttgtgaaatt tatagcgtag attttcatga aaatattcaa atataatttc
    18601 atgaggcgcg cgccatgttc aaacaatccg cccagcatat cggcacctac tacgccggca
    18661 cctacccagg ccagatcccc ctacgcccga gcctgcagga agcgttgcag tgtgatgtgc
    18721 tgattatcgg cggtggtttc agcggcctac acacggcgct gcgcctggcc ctggcgggca
    18781 agaaggtggt gctgctggaa gccagtcgcc tggcctgggc cgcctctggg cgcaacggtg
    18841 gtcaggcgct gccgggttgg tcctgcgata tgccgccgtt cgagaaggcc ctcggcctgg
    18901 aacgcgcccg ccgcctgtgg gacagcatgg tctgggccac cgaagaaatg cgcgaactgc
    18961 ccgagcgcca caacttcgat atcgattacc gcagcggcgc gatctacacc gccgtgctgc
    19021 cacgccgtgt gcgccagttg caggacagcc tggaagaagc cgagcagaaa tacggctata
    19081 cgcagctgag ctttatcccc aaggagcaat tgccagagtg gattgccagc ccgcgttacc
    19141 tggctgccct gcatgacagc ggtgccgccc accttaaccc gctgaaactg gcccagggtc
    19201 tggccgatgc catcgagaag gccggcggaa aaatcttcga gcagagtaag gcgctgagtt
    19261 atcaagaaac cgcggacagt tatgtagcgc gcaccgagca gggcgaggta cgcagcgacg
    19321 tgctggtact ggcctgcaac gcctatatcg acaagctcga tccacagctg tcgaaacgcc
    19381 tgttgccggt cggctcctac caggtcgcca ccgcgccgct ggacccggag ctggcgcgct
    19441 cgctgttccc gcgtggcacc tgcgccatcg acaaccagtt cgtcccggac tacttccgcg
    19501 ttaccccgga caaccgcctg ctgttcggcg gcggctgcac ctacctgggc ggtattcctc
    19561 aggacgtgcc ggcggccact cggccgtatc tggagcgggt attcccgcaa ctgcgcggcg
    19621 tgcagatcga ctacggctgg ggcgggcata tcgactgcac catgcaccgc accccggacg
    19681 ttggccgcag cggccaaaaa tactggctgc agggcttctc cggccacggc atcctgccga
    19741 ccctggccgc cgcccgcgcg gttagcgacg cgatcctcgg tggcgccgac ctgcttgagc
    19801 tgtaccaggg cctgagcaac ccgcgcttcc ccggcggcga tctgctggcg gcgcccatcg
    19861 aggccgtggg caaggcttgg tatcgcctgc gcgatgtaat ttaaggaagt tgaccatgga
    19921 catgcaggaa gaagtcgaag gtctggcgat cctgatccgc gacctgcgca agcacaaaaa
    19981 cctcaccctg ggtgagctgg ccgcgcgtat cgaccgttcg gtgggctttc tctcccaggt
    20041 cgagcgcggc ctgtcgcgtc caaccgtggc cgacctcacc gcgatcagcg aaaccctcgg
    20101 cgtgccgacc acctatttct acagcctgag caaaccgcgc gacgtgccct gggtcacccg
    20161 ccccggcgag cgccgcacgc tgtattacgc cggcggcatc accgacgtgc tggcctcgcc
    20221 aaccatggcc ggcggcttct ccatgctcga aagccatctg cagcccggag ccaccagcgg
    20281 tgatgggcat ttaaacgaca gttccgagca gggcggcttc gtcctcgaag gcgagctgac
    20341 gatctggtac ggcgacaccg ccgagccagt cacccttggc ccgaacgaca gcttccagct
    20401 gccgccgcac gcgcagtttc gctacgccaa cctttccgat tcccccacac gagtgctttg
    20461 ggtcttcacc tgacccgcgc agatagccgc catgacaata acaacagcat ttcccgacct
    20521 gctcagcgaa gtccgcgcct tccgtagcgc caaccccgaa gtgcgctatg tcgacctgat
    20581 cagcctggac atccccgggc atttctacgg caagcgctac cccatcgaca tgctcgagaa
    20641 ggttgccgcc ggcagcccgc tgaaaatccc gcagaactgc gtgctgcttg gcgtgcaggg
    20701 cggcctgcac ccgattggcg actactgctt caacgacggc gacccggacg cgccgcgccg
    20761 gctgattccc ggcacgctca agccggtgcg ctgggaaagc cagccgctgg ggcagatgct
    20821 gatcagctcc gacggcactg acgcgcccat cgagttcgaa ccgcgtgaag tgctcgcacg
    20881 cgttctcaaa cgcctggccg cacgcggtat ccgcccagtg gtggccttcg agctggagtt
    20941 ctatctgttt gatcgcaagc tacaggacgg cctgccgcag ttcccacgcg acccgctgtg
    21001 cgacgatgcc gatgaccagc cgaatatgca tatcgaacgc ctgtcgcgct ttagcgatgt
    21061 gctgcacgag atcgtcgatg ccgccaatga gcagggcgtg gacgccaacg tgatcaccgc
    21121 cgaactcggc cccggccagt tcgagatcaa cttcggccat tgcgacgacg gcctacgcgc
    21181 cgccgactgg gccgctttgt tctgccgcag cacccgtggc gtagcgctca agcacggcca
    21241 gcgcaccagc tttatgagca agccctacct gcacgcgccg ggcagcggca tgcatgtgca
    21301 cgtcagcctg tatgacgccg ccggcaataa cctgctcgcc gccgatgagc aacgtccgct
    21361 gcgccatgcc gtggctggct gcctggagct gctaccgcat tgcatgccga tcttcgccgc
    21421 caaccacaac gccttccgcc gctacggcgc catggtcaac gccgccagcc gcgccagctg
    21481 gggcttcgag gaccgcgatg cgtgcatccg catccccgaa tcggacggca agaacctgcg
    21541 catcgaacac cgcctggccg gcgccgacgc caacccttat ctggtgctgg cggcgattct
    21601 caccggcatg gagcacggcc tggatgccgg ccaggagccg attgctccac tcaacgaaaa
    21661 ccgccagagc ggtatcgact tccctcagga catgctcggc gcggtcagcg ccatgcgcga
    21721 tcatccggtg gtcaaccagg gccttggcag tgagttcgtg atggtctact gcgagaacaa
    21781 gcgccaggac cacctggcct ttatgcagga agtcagtgcg cgggaatacc gctggttcct
    21841 ctagccgtca cacggccatc gcgtagcccg gataagccct ggcgcaatcc gggaaccacc
    21901 ccggattacg ctggcgctta tccgggctac gggcaggctt ctgggaacct tagtccgcag
    21961 gtccagcttc aacgcaacag atgcaccgca agccccgcta gcgccgacac cagcaacact
    22021 tccatcaccc cacgcttgaa gcgcagcagc gccagcgccg ctaccatggc aagcagcgcc
    22081 gacggccagt cgaaagtgcc gccgaagccc tgcggccaaa gcacgtggta ggcaaagaac
    22141 aacgccagat taaggatcac cccgaccact gcggcagtaa tcgcggtcag cggtgcggtg
    22201 aatttcagct cgccatgggt ggattccacc aacggcccgc cggcgagaat aaacaggaag
    22261 gacggcagaa aggtaaacca ggtgaccagc gccgccgcca gtgcgccgct gacaaaaccc
    22321 gaatcgccgc cgaacaccgg ctgcagatag gccccaacaa aggccacgaa cgccaccacc
    22381 atgatcagcg gccccggcgt agtttcaccc agcgccaggc cgtcgatcat ctgcgtcggg
    22441 gtcagccagc cgtaatggcc aatggcgccc tggtagacat acggcagcac cgcataggcg
    22501 ccgccgaagg tcagcagcgc agccttggta aagaaccagg ccatctgcgt cagcgtgccc
    22561 tcccagccat acagcacggt caacaggctc atcggcagca gccagagcag cgcgcccacc
    22621 gccagcaaca gcgccaggcg cgacgcgcga aagcgcgtat gcggcaacgg cgggttgtca
    22681 tcatcgatca gcgccggccc ataagacttg ccctgttgcg catgcccacc acccaggctg
    22741 aactgctgcg gcagcaggcg gccaccgagg taaccgagca gcgcggcgcc aagcacgatc
    22801 aacggaaacg gcagattgaa ggcaaaaatg gcgacaaagg acgccccggc aatgccccac
    22861 agccagttgt tcttcagcgc ccgtgagccg atgcgccagg ccgcctgcat gacgatggcg
    22921 gtgaccgcag gcttgatccc gtagaagatc cccgccacca gcggcacatc accgaaggcg
    22981 atatacagcc aggacaggcc gatcaggata aacagcgacg gcagcacgaa cagcaccccg
    23041 gcaatcacgc cgccccaggt gcggtgcatc aaccagccaa tgtaggtggc caactgctgc
    23101 gcctccggcc ccggcagcag catgcagtaa ttcagcgcat gcagaaaacg ccgctcggac
    23161 agccagcggc gcttctccac caactcctga tgcaggatcg cgatctgccc ggccggccca
    23221 ccgaaactga tcaggccgag cttgagccag aaccagaaag cttcacccag gctgacggtg
    23281 ggcggcgcag gctgtgcaga ggattcggac atgcgggcaa ctcacggcaa taaaaacggt
    23341 cgcactatag cgcgagcgca tgacgattta cgccggcacg gctcaagcca aggcacggat
    23401 ttccgtatac ttggcagcgc tgcgccctcc ttccaacctg cccgtgaagc tctctgtgaa
    23461 aatccgtcat tcgatagtaa gcctgctgct gatctgcagc tgcgttgtag ttgcatcgaa
    23521 cacccaggcc gcccccgcca aacaacagga cctggccgcc ggcagcgccc tgctggtcga
    23581 tctgcagacc aatcaggtgc tctacgagcg caacgccgac agcgtggtgc cgattgcctc
    23641 ggtaaccaag ctgatgaccg ccatggtcac cctcgacgcc aagctgccgc tggatcagca
    23701 gctgccgatc atgattcgcg atacccatga catgcgtggc gtgttctccc gcgtacgcgt
    23761 gggcagcgaa ctgagccgcc gcgacatgct gctactggcc ctgatggcct cggaaaaccg
    23821 cgccgccgcc agcctggcgc accactaccc gggtggccac gcggcctttg tcgcggcgat
    23881 gaatgccaag gcccgcgccc tgggcatgaa gaattcgcgc tttgtcgaac ccaccgggct
    23941 gtcggagcac aacgtctcca acgcccgcga tctggtgctg ctgctcaagg ccgcacggca
    24001 gtacccgctg atcagccagt tcagcaccac ttcggaaaag acccaggcat tccgcaaacc
    24061 caattacacc ctgggttttc gcaataccaa caacctggtg cgcaagcccg gctggagcgt
    24121 gcagctgagc aaaaccggct acaccggcga agccggccgc tgcctggtga tgaataccgt
    24181 gatgaataac cgcccggtag ccttcgtggt gctggatgcc ttcggcaaat acacccatat
    24241 ggccgacgcc aaccgcctca agcgctggct ggagaccggt aaggtcaccg ccgtaccgcc
    24301 cgcagccatc agctaccgcc agcagaaact ggcgcagcgc caggccagtg cggcgcagta
    24361 gaaaaccgac aacaggtcgg atcaacacgg cgctccgccc gtataaccga ggggttactg
    24421 aaagcgtccg acctcttgac gcagctcttc ggccagctga tccagttcac gggcagtttg
    24481 tgcgaggttg gcgaccactt cgcgttgttc gctgttagcc aacgcgatgc tctgcaggtt
    24541 gctgctgagc aacgttgcgg tactgctctg ctcgctggta gcagtggtga tagcggtaaa
    24601 ctgttcaccg gccgcactac tttgctcggc gatctgttcc aacgcagcgg cgaccttggc
    24661 attgcgctgc aacccgtcct gcattagtga cttgccctgc tccatggtgc tgatcgcgct
    24721 gttggtctcc ccctgaatac tgccaatcat tgcggagatt tcgttggtcg cctcacgggt
    24781 acgggaagcc agactgcgga cctcatcagc aaccacggca aaaccacggc cctgctcgcc
    24841 agcgcgagcg gcttcgatgg ccgcgttgag cgcgagcaag ttggtctgat cagcaatcga
    24901 ggtgatcacg ctgacaatcc caccgatctc ctgcgaacgc tgacccagtg tgttcaccac
    24961 tacggacgtg tcatttagtg agctggcaat ctgctccagt gcgctcgaag cctcagccat
    25021 cgaactgcgt ccaatacggg tctgctgggc attgtcgctg gccaggcgtt cggtattgcg
    25081 catgttgtca gcaatgttca gcgaggtggc gctgaactct tccacggcgc cggccatgct
    25141 gctgatctcg ccagactgtt gctcgatacc ctcataagca ccgctggcca gaccggacag
    25201 ggcctgagca cggctactga cgttgtgcgc ggcgccgcga atgcgcgcca ccatagtcga
    25261 caatgcctcg cccatctgat tgaagttgcg cgccagctca ccgatctcat cctgactagt
    25321 gacgttaaga cgcgcactca gatcgcctgc acccaatgcc tgcgcctgtt gcaccagctc
    25381 accaagcggg cgcagtttgc gccgcaacaa ccagatcact gccgcgaccg ccagcaacaa
    25441 cgtcaccagg ctgccgatcg ccaactggct gcccacgtgc caggttaccg cctggatttc
    25501 ttcacgcggc atgctcgcta gtacttgcca ggggccaccg gaaaacggca ttgcaacact
    25561 gaagaaatcc tccacgccgt cgctccagaa cgccccctgt cctggctcag ccagcacatc
    25621 ggcgagcttg ccggcaatgt gctcaggctc ccgcgcacca gcgggtggca ccagccattt
    25681 accctgatcg tcgagtaacg cgagggagcc cgtggcgccg atgcggaaac gtttgaggtt
    25741 ttcaaactgc atgttctgcg catcggtgta gtcgaagcca acgaacagta ccgcaatcac
    25801 ccgcccactg ctgtcgcgca cgggggtgta ttgagtcatg taaaaccgct caaacagcaa
    25861 tgcccgtccc acatatcctt cgccactcag caggcgcctg tatgccggat gctgatggtc
    25921 gagtgccgtg ccgatggcac gagtgccgtc aggcttggtc agcgaggtgc tgatgcgcac
    25981 aaactcatcg ccatcacgga cgaacaccgt ggccacaccg gcggtcagct gacgaaactc
    26041 atcgacttcg ccaaagttgt tgttcaacag ggccgagccc aggtacagcg ccggcgcttg
    26101 catgtcggcc acggcaattc gctcagcccc ctgcacactc aagccaccac tgaaacgccg
    26161 ctcgaacagg ccagcaaggc gctgagtgct ttcgcgcagg ctgtcatgga aggtgctcag
    26221 ttggtcggcc aacagacgcg cctcactccc caaatgctcc tgtcgtgtga ccaggttggc
    26281 ctcgctcagc gagcgcagcg cgaataacgt actgacgctg atcaacagga ccaggaccag
    26341 agccaacgcc gtggcaagct gccaggcaat ccgagaacga ggggaggtca tgcatgcctc
    26401 caaaggcata aaacggcagt tatagggtat gaaaaggcca aatcagctta aatctagcaa
    26461 tcggcgagcg cggagaatac ttgagtaaac gactaagact atagccaact cgttacgcag
    26521 agatcgctct gagcagtggc accgccactt cagacaaaca tgcgaaagct gcggcttctt
    26581 ggattgctgg gagtgggtat ctggcgaggt gtgttaggca gtatcggcac tgccttagcc
    26641 gttaaggcct aacaagggct ggcgcatgcc aaacaccaga aaacgcgcca gctcggcgag
    26701 cggcaaggcc ttgctgatca ggtagccctg tacctggtcg cagccgtatt gacgcagcag
    26761 cgccagttgt tcggcgtttt ccacgccctc gcccaccacc tgcaggttga ggttgtgcgc
    26821 caggttgatc atcgcccgca ccagttgacg gttctccgcg cgctcgccca tgccaccgac
    26881 aaagctcttg tcgatcttca gcagggtgat cggcaggttg ttcaggtgga cgaaggaaga
    26941 gaaaccggtg ccaaaatcgt ccagcgagaa gcgcacgccg aggcgaccga gggccagcat
    27001 ggtctgctgc acctgatcgc tgcggcgcat caccgcggtt tcggtcagtt caaactccag
    27061 ccactgcgca tccacgccgc gctcttcgat caggcgacta agggtcggca gcagctggct
    27121 gtcctggaac tggcggaacg acaggttgat cgccatatgc agcgccggca ggccacgccc
    27181 gcgcagccac tgcatatcgc gcagcgcgcg gctgatcacc cagtagccga gcggcacgat
    27241 cagcccgctt tcctcagcca gcggtacaaa ttcgtttggg gccagcaaac catgttcgct
    27301 gtgacgccag cgcaccagcg cctccaggcc gacgatgcgc ccggtctgca aacacagacg
    27361 cggctggtaa tgcagttcca gctcgtcgcg gcgcagggca cggcgcagct cgctttccag
    27421 atcggcctgg ttgcgtgcgc tgcgattgat tcgctcgtca tagatatgaa aggtgcagcc
    27481 ctgctggctc ttggcctgct gcatggcgat atgcgcgtgc cacatcagcg gatcggcgct
    27541 gccttcggcg cgggaatgcg ccagacccag gctgcagccg atcaacaggc tttcgccgtc
    27601 gatccagtac ggttcgccga gggcttcggt aatttgcgcg gccaggcgct cggcgcgctg
    27661 cgggtcgcgg cggctgtcga ggagcaaggc aaattcatcg ctgcccaggc gggccacctg
    27721 gtcgccggcg tgcagctgcg ccttgatccg cgccaccact tgcagaatca ggctatcgcc
    27781 gccctggtaa ccaagggcat cgttggcgtg acggaagttg tccaggtcga gatggccgag
    27841 ggtcaggcca cggccttcat attcggccag gcgcgcagca agcagggtct gaaagccctg
    27901 gcgattggcg atgccggtca gggcatcctg ttcggccagg cggtgcaagg tgtcattgag
    27961 gctgccgcgt tcgcgcacat agcgcaggca gcggcgcagc acttcgactg tgagctgatc
    28021 gcgcaccagc cagtcgcaca cgccctcagg ctccgcttcg ggctcggcat ccagcagcag
    28081 gacaattggc agggagcagc gcccggaggc aggcaggtat ttggcggtgg tcaggagcac
    28141 ggcgttgctg ggtttatcga acagcgcgct ggccgcttcc caagacggtg cggtaatcag
    28201 cgcatagcca ctaccgagcg cactcaggcg ttcgcgcagc aactgcgccc agcccggcgt
    28261 ttccgccagc aaaagcaacc gtaatggttc gacgaacaca gacaagcaac ttcccccacg
    28321 gcacaaataa agagcattaa acccgctcgg atgcgtcgta atcgacgtct aatcacgcac
    28381 atccttgtgt cagtggcgcg cgctacgcag tatggcaagg tccagaaagt ggcaccaggg
    28441 tactggatta ggaaaattaa ccaagcctgg cgacttgcca ttatgtgcac tgcaacacac
    28501 ttccagatgg tcacagcaga aagcgccagg cctgttaaaa tgcgcgccca ttccgcctga
    28561 cgattgactc catgtcccga ctgaaccccc ggcagcagga agccgtgaac tatgtcggcg
    28621 gcccactttt ggtgctcgcc ggcgcaggct ccggcaaaac cagcgtgatc acgcgcaaga
    28681 tcgcccacct ggtgcagaac tgcggtatcc aggcccgcca tatcgtcgcc atgaccttta
    28741 ccaacaaggc cgcgcgcgag atgaaagagc gtgtcggcac cctgcttaag ggccccgaag
    28801 cccgtggcct gacggtgtcg accttccata acctgggcat gaatatcatc cgcaaggaat
    28861 atgcggcgct gggctacaag ccgggcttct cgatcttcga cgagggcgat atcaaggcgc
    28921 tgctgtcgga catcatgcag aaggaatatt ccggcgatga cggcgcggac gagatcaaga
    28981 actacatcgg cagctggaaa aacgatctga tcctgcccga acaggccctg gccgagtcgc
    29041 gcaaccccaa ggaacagacc gcagccatcg tctacctgca ctaccagcgc acgctcaaag
    29101 cgtacaacgc ggtggacttc gacgacctga tcctgatgcc ggtgaagctg tttcaggaca
    29161 accccgacat tctggaaaag tggcagaacc gcatccgcta cctgctggtg gacgaatacc
    29221 aggacaccaa cgccagtcag tacctgctgg tgaaactgct ggtgggcatg cgcaaccagt
    29281 tcaccgtggt cggcgacgac gatcagtcga tctacgcctg gcgcggcgcg cgcccggaaa
    29341 acctgatgct gctcaaggac gactatccgt cgttgaaagt ggtgatgctc gagcaaaact
    29401 accgctccac cagccgcatc ttgaaatgcg ccaacatcct catcgccaac aacccgcatg
    29461 cgttcgagaa acagctgtgg agcgagatgg gcatgggtga cgagatccgc gtgatccgct
    29521 gcaagaacga agacgccgag tgcgagcggg tggccctgga aattcttacc gagcacctgc
    29581 gcacccagcg cccctacagc gattacgcca tcctctatcg cggcaactac caggccaagc
    29641 tgatggagct gaagctgcag caccatcaga ttccctatcg cctctcgggc ggcaccagct
    29701 tcttcgcccg ccaggaggtg aaggacctga tgagctactt ccgcctgctg gtcaacccgg
    29761 acgacgacaa cgccttcctg cgggtgatca acgtgccacg ccgcgagatc ggctcgacca
    29821 cattggaaaa actcggcaac tacgccacca gccgcaagat cagcatgtac gccgccgccg
    29881 gcgaaatcgg cctgggcgag cagctggacg cgcgctatac cgaacgtctg gggcgcttta
    29941 ccaagtggat ggaccgggtg cgccaggagt gcgcgcagaa cgacccgatt gcggcgatcc
    30001 gcagcatggt catggatatc gactacgaga actggctgcg gcagaacgcc tccaacgaca
    30061 aggtcgccga cgcgcgcatg ggcaacgtct ggttcctggt cgaggcgctg aagaacaccc
    30121 tggagcgcga cgaagacggc gagatgacca tcgaggatgc catcggcaaa ctggtgctgc
    30181 gcgacatgct cgaacgccag caggaggaag aagacggtgc cgaaggcgtg cagatgatga
    30241 ccctgcacgc gtccaagggc ctggaatacc cttccgtgta tatcctcggc gtggaggagg
    30301 aaatcctgcc gcaccgctcc agcatcgagg ccgatactgt tgaagaagaa cgccgcctgg
    30361 cctacgtcgg cattacccgc gccaaacgca acctgaccat gaccttcgcc gccaagcgca
    30421 agcagtacgg cgaaatcatc gactgctcgc cgagccgttt tctcgatgaa ctgccgcccg
    30481 aggatctagt ctgggaaggt ctggaagacg ccccacccga ggtcaaggcc gccaccggca
    30541 actcggcact ggccaatatg cgcgccatgt tgaaaaaatg accgggagtc atttttaacg
    30601 tcgcttgcga cggcccgcag ggtggccgcc agggatggca ggccataaaa aataggtttg
    30661 ttcagtagcc cgacgcagag cgggtagcac atcacttaat gagggttacc gcgtggaagc
    30721 gctaaaacag aagattcgcg atgaaggcac cgtgctctcg gagcaggtgc tcaaggtcga
    30781 tgcctttctc aatcaccaga tcgaccccaa gctgatgcag cagatcggcc atgagttcgc
    30841 ccggcgtttc gccggcttag gcatcaccaa gatcgtcacc atcgaagctt cgggcattgc
    30901 cccggcggtg atggccggcc tggagctggg cgtaccggtg attttcgcgc gcaagtacca
    30961 gtcgctgacg ctcacggaca acctgtatat ctccaaggtg ttttccttca ccaagcagac
    31021 cgaaagcacc ctggccattt ctgccaagca cctgaacgcc aaggatcacg tgctgctgat
    31081 cgacgacttc ctggccaacg gccatgccgc ccgcgcgctg atcgacctaa ttggccaggc
    31141 cggcgcgagc attgcgggta ttggcgtggt gatcgagaag tccttccagc gcggccgtgc
    31201 cgagctggat gcacagggtt atcgggtcga gtcgctggca cgcatcggct cgctgagcga
    31261 tggccaggtc agctttctcg actaagccta cgcggcttca gctgctggcc cgcaagccgg
    31321 ccaacagcag gcgccgctgc acacgctgac gcaacccacg cggcggggcc aacgccatgc
    31381 gttgcagatg cgcgtcgtac ttgccagggt tgggcgcgcg ccatattgca acggccaact
    31441 ccagggcaca ggcgaatctc agtttgctct tcagccaact cagcgcgcgc aacagatcac
    31501 gctcctcgtt atcgaaatcg cagcccaagg ggtattcggc gaacagtccc ggatgacagt
    31561 tctgcaccgc ctgcaagcgc tcgggcaggt taccggcgaa gcgcggattg aggcggaaat
    31621 ccttgggcag tttgccagct cgttgcgcct gttcgatcaa acccgactga aagcgcgagt
    31681 cgctgatatt gagcagcgct tctatcacct gggagtcagt cttgccgcgc agatcggcga
    31741 tgccgtattc ggtcaccacc atgtcgcgca ggtgtcgcgg aatggtggca tgaccatagt
    31801 cccagacgat attcgagctg accttgccgc ctgcctggcg ccagctgcgc aacagcagaa
    31861 tcgagcgagc accctgcaag gcatggccct gggcgacgaa gttgtactgt ccacccacgc
    31921 cactgagcac ccggccgtct tccagttggt cggccacacc ggcgcccagc aaggtcatgg
    31981 tgaaagcggt gttgataaag cgtgcatcac ggcgctgcaa acgcttgagc gcttcctggt
    32041 tatgcagctc gttgataaag cagatgcccg tcatggcaaa gcggcccaac tgcactgcgc
    32101 tcaattcgca aaggcgttgg taaaaggctt gcggcccgag gaagaagccg ccgtgtatca
    32161 ccacgccgcc gcgtgcccgc cccagatgtt gctgcaacac ctgctgggtg cgcgcaccgc
    32221 gcaggtcgct caacagctgt tgcccgtccg gcattagcag gtgaccatcg gcccagcgaa
    32281 tactctcggc cagcaaaccg ctggcctgca acccttgcaa cgcctgggca tccaattgcg
    32341 gcgccaggcc cgcatcgagc agagcctgca cactgcgcgg cataccctga gcatccaacg
    32401 cgccgccaga ggccagctgc tgcaggtgca attcggggta taccgggcgc cgcacgatgc
    32461 ccgcatcggc caaggccagc aggccattga cgaacatctc gctgcagccg tacaggccct
    32521 cgctaaatgg ctgcaagccg ccatctttag cgatttcggg ggcccaatga atggtgcagc
    32581 gcagttcatt cagcagcgcc cgatacgcct ggttatccgc ctgacgcgcg agcaaggccg
    32641 cggtcagcgc atcgcccatg gcgccgatgc cgatttgcaa ggttccgccg tcgcgcacca
    32701 aagcgctggc atgcaggccg atgcagtgat cctgcagcgt caccggcata ttcggtgtgg
    32761 aaaacagcgt gctgcggtcc tcttccaaca ggtgcaggtc gaagctgcgc cgcgatactt
    32821 cggcatcgcc gaccatatag ggcagcgcgc tgtgtacctg ggccaggcac agaatggttt
    32881 caccggcggc gcggcgctgc tccaggagcg gcagcagatc cagggtgata tccgggttgc
    32941 agctgaggct gaaatgctcg gggcgctgcg gatcatgcgc gaccagttgg gcgatcaggt
    33001 tcaggccctt gtcattcagg tcgcgggcga catggctgta attgctgctg atgtagttct
    33061 gctgcgtcgt cgcgctgtcg agcaggctgc caggctgcag aaagaactgc tcgacacgca
    33121 cgttggccgg caaggtgtcg cgctgcaagt cggcgagaaa atccagttcg ggataatcgc
    33181 catagatacg ttcgacgaat ggctcaagca ggcggctcgg caaacccgca gcggcccgcg
    33241 ggcggcccag gcatagcgcg gtgtagatgg tcagctggcg ctgcggcagt tgcttgatgc
    33301 gcgcgtagag tgcattgacc cactgattcg gcttgcccag acccaggggc aggccaaggt
    33361 gaatagggcc gtcgatggca ctgaggacgt ggtcgaccgc ctgctcgatg gtacacgtct
    33421 tcggcatcgg gctctctcct ggcatcgtta acggggctat cgcccattat tgccgcaacg
    33481 ccgcgccaaa acgaaaaagc cgccctggca ggcggctttt tctacagcta ttgcgtgtgc
    33541 ttacagaccg gacatcttct ggattgcgcc acgcagctcg tcatccgagc aatcggcaca
    33601 ggtgcctttt ggcggcatgg cgttgatgcc ggaaatcgcc ttggccagga tgccgtcaag
    33661 accaccctct gcatcagcac gggctttcca ggctgcagcg tcgccaattt tcggcgcacc
    33721 cagaatacct ggggtgtggc aggcgccgca gtgcttggtg atgatgtcat cggcagtacg
    33781 cgcaccgccg ccagcagcaa cggctacagc acccacgccc ttgcattctt cacccatcac
    33841 acagacttga cctaccggct tcagtcgctc ggcaatggct tcgtcggtcg tggcttgagc
    33901 agtcactgcc cacagggcca atacggtagc cggtgctacc aggagctttt taatcaggtt
    33961 cacggtacac cctcttcgtg gctagtcacg ctcgcggcca cggtttcgcg agcgggcgtc
    34021 agtataacgg caagccgccg ccgctgaaac aacccatatt gtcgcagggt tatttagaag
    34081 ttggccggcg tagtcgcgct aatcagccgc gctggctgct cgaacggatt gcggaaacgg
    34141 tgcggtttgc tgctctcgaa gtagtagctg tcgcccggtt cgaggataaa aacctcgtcg
    34201 cccacggtca gctccaactt accttcaacc agcatgccgg cctcttcacc ttcgtgggcg
    34261 tacatctcgt cgccggtgtc ggtgccgccg gggtaggttt catcaaggaa ggcaatggct
    34321 cgggtcggat gtgccttgcc caccaacttc atggtgatgg ccccgctgca gatatcggtg
    34381 agttcggcgg cgcgatagac cacctgggcc tggctatcct gctccacatc cagggagaag
    34441 aactccacca gcgacatggg gatacccgcc aacacctttt tcaacgaact gatcgaaggg
    34501 cttacgctgt tcttctcgat catcgaaatg gtgctgttgg tgacgcccgc ccgcttggcg
    34561 agttcacgct gggaaaggcc tttgagtttg cgaatggatt gcagtcgtac accgacgtcc
    34621 aatgctggag tcccccttgg gatagatacg gaaaagtcgc cgtatcatgg catagcgttc
    34681 ggaatttaca acactcgtat tcaaactcga ctacagcgct ggggttaact cccaagatag
    34741 agcagcggcg cccgccgcag gttgcaaaaa agctggtagg gaatgctgcc ggcctgggcg
    34801 gctacgtcgc tggccagcac ctgtttgccc cagagttcga cccggctgcc caagcccgct
    34861 tgcggcagat cggtcagatc aacggtgagc atgtccatcg acacgcggcc gatcaggcgg
    34921 gtcagttggc cgtccaccgc cactggcgta cctgtcggcg cgtgacgcgg gtaaccgtcg
    34981 gcatagccca tggcgaccac accgacgcgg gtcggccgct cgctgaaaaa gcgcgcgcca
    35041 tagcccaccg gctcgccggc cggcagttcg cgcacgctga tgatcttcga ttccagggtc
    35101 atcaccggtt gcagacgcgc ggccacggcc tgtgcctgct cgaagggggt ggcgccgtag
    35161 agcatgatgc cggggcgcac ccagtcactg ggtacgttgg gccaacccaa tacggctggc
    35221 gaattgcgca ggctaacttc agcgacaatg ccctgacgcg cctgctggaa caccgccaac
    35281 tgctcatcac tgcgcgggca atccagttca tcggcgcggg cgaagtggct catcagcaca
    35341 atcttcgcca ccttgccgct ggccagcagg cgctgataag ccgcctgata atcggctgga
    35401 tgcagaccga cgcggtgcat gccgctgtcc atcttcagcc agacggtcag cgggccacgt
    35461 acagcgctgc gctcaatcgc ctccagttgc cacagcgaat gcaccacgca ccacagctcg
    35521 tgctgctcga tcagcggcaa ttcgtcggcc tcgaaaaagc cctctagcaa caaaatcggc
    35581 ccgcgaatac cggcctcgcg cagctgcagc gcttcctcga tacaggccac ggcaaaacca
    35641 tcggcctgct cctgcaacgc ctgggcacaa cgcaccgcgc catggccgta ggcatccgcc
    35701 ttgatcactg ccagggcgcg cgcgccgctg acttcacggg ccagttggta gttgtgacgc
    35761 aaggcttgca gatcaatcag ggcacgggca ggacgcatga tgttctcggc tatcagcagt
    35821 tttcaggctt aaaaaaatgg tcgggagcta tttttaacgt tgcgtagcaa tggcccgaag
    35881 ggtagccgcc agggatggca ggctataaaa aagcccggtg tgatggccgg gcaaacactt
    35941 atcgcaaacg ggatgggccg atttacaggg cggccacaat catcatttcc accagcacct
    36001 caggcttgta catcttcgcc tcgacgcagg cgcgtgcggg tgccgcgccg ggcgcgaccc
    36061 aagcatccca gacctgatta agcccggcat agtcgcgctc catgtccttc aggtatatgg
    36121 tcacggagag gattttcgac ttgtcgctgc cggcctcggc cagcatgcgg tcggtgttgg
    36181 ccaggttttc gcgggtttgc tgcacgatgt cgccgctgta atcgtccgcc agttggccgg
    36241 ccaggtagac cgtgccgtta tggatgacga tttcgctgta acgactttca gtgtgcaggc
    36301 gctgtactga catgcttatg actctcctgg gaacggctgt aacgggaaat atccagacct
    36361 tcggcgctga tctgcgggcg ctttttcgcc atcagatcgg caagcaggcg accggagccg
    36421 caggccatgg tccagcccag tgtgccgtgg ccggtgttga ggaacaaatt gcgcaaaccg
    36481 gtggcgccga caatcggcgt accgtccggg gtggccggac gcaggccggt ccagaattcg
    36541 gcctggctca gatcgccgcc ttgcgggtag agatcggcgg taatcatttc cagggtggcg
    36601 cggcgacgcg ggttgaggct taggtcgaag ccagcaattt ccgccatgcc gccgacgcgg
    36661 atgcggttgt cgaaacgggt gatcgccacc ttgtaggttt catcgaggat ggtcgaagtc
    36721 ggcgccatcg ccacattggt gattggcacg gtcagcgagt aacccttgag cgggtacacc
    36781 ggcgccttga tgcccagcgg cttgagcagt tgcgggctaa agctgccgag cgccagcacg
    36841 tagcggtcgg cagtttccag cttgccgtcg atccacacgc cattgacccg gtcgcccacg
    36901 gcgtcgatgc gttgaatgtc ctggccgaag cggaattcca cacccagcgc cttggccatc
    36961 tcggccaatt tggtggtgaa cagccggcaa tcgccggtct ggtcgttggg cagacgcagc
    37021 gcgccagcca gtttgtcggt aacggcggcc agggccggct cgacgcgggc aataccggcg
    37081 cggtcgagta cttcataagg cacaccggat tgctgcagta cggcgatgtc tttcgccgcg
    37141 ttgtccagct gcgcctgggt gcggaacagc tgggtggtgc ccaggctgcg gccttcatag
    37201 gcgatgccgg tttccgcgcg cagctcgtcg aggcagtcgc ggctgtactc ggacaggcgc
    37261 accatgcgct ccttgttcac cgcgtaacgg ctggcggtgc agttgcgcag catctgcgcc
    37321 atccacaggt actggtcgat atcggcggtg gccttgatcg ccagcggcgc gtgcttttgc
    37381 agcagccatt tgatggcttt cagcggcaca cccggcgcag cccaaggcga ggcgtagcca
    37441 ggagacacct gaccggcgtt ggcgaagctg gtttccatgg ccggggcgtg ctgccgatcg
    37501 accaccacca cttcaaagcc ctgacgtgcc aggtaatacg cactggctgt accaatcaca
    37561 ccgctaccga gaaccagaac ccgcatgccg ctctcctcgc cgcgattgag cgcgacggat
    37621 aatttctgag atcacaattg agcgcagtat agaaagagaa cagcagtgct tttcactata
    37681 taaatagcta tatttggcga caattcttgg caatatcgcc ttctgtagag gggtatccac
    37741 catgcgcacc caacatcaaa gccgccgcga actggataag atcgaccgta atatcctgcg
    37801 catcctgcag gaagacgggc gcatcagctt taccgagctg ggcgagcgag tcggcctttc
    37861 caccacgccc tgcaccgagc gcgtacgccg cctggagcgc gaagggatca tcatgggcta
    37921 ccacgcccgg ctcaacccgc agcagctcaa ggccagcctg ctggtgtttg tcgagatcag
    37981 cctggactac aagtccggcg acacctttga ggagttccgc cgcgccgtgc tcaagctgcc
    38041 gcacgtgctc gaatgccacc tggtgtccgg cgacttcgac tacctggtga aagcgcggat
    38101 caacgagatg gccagctacc gcaagctgct cggcgacatc ctgctcaagc tgccgcacgt
    38161 gcgtgagtcg aagagctaca tcgtgatgga agaagtaaaa gagagcctaa gcctgccgat
    38221 tgccgactag accagtacct ggcgcgtggt agcgatcaac tgatgcacct gctttgccac
    38281 cgccggctca atcatctgct ccggcccctg gccacgcggg cacggcaggc ttggcgtggt
    38341 gccgaacagg cggcagatca gcgggcgctg gtcatacacc gtgcagcctt gcggaccgag
    38401 gtgtacacag ttgtactcgg ccagggccgc gtcgtgttcg gcagcgcttt tcaccggcag
    38461 ccgcgacatc tcctcggatg acgccgtaac cgggccacag caatcatggc agccgggtat
    38521 gcaggcaaaa ctgggaatct gcaaacgcag ctggtcgatc ttgcggctgg tgcaactcat
    38581 gggcgtgatc ctgaaacaca ggcaggcata gtaaccgcgc agcgggctga atcgcggcag
    38641 ctgcgcgccc aaccgacgaa aatccactta tttcgcgatt gcctcttggc gtttggcaaa
    38701 tcccacgctt atccttcgta aaattttcca aacacaataa tcaggattcc acacatgaac
    38761 gcccgcgttc accagccggt tcacaacagc cagcacgcca actcctatta cgccaccagc
    38821 gccaatcgcc aactcggcta ccccgccctg ggcggcgagt tgcgcgccga tgtgtgcatc
    38881 gtcggcggtg gtttttccgg ccttaacacc gccatcgaac tggcgcagaa aggcctttcg
    38941 gtggtgctgc tggaagccca caaaatcggc tggggggcca gcgggcgcaa cggcggccag
    39001 ttgattcgcg gcgtcggcca tggcgttgag cagtttgaat cggtgattgg cgcgcagggc
    39061 gtacgcgagc tgaaactgat gggcctggaa gcggtggaaa tcgtccgcca gcgtatcgag
    39121 cagttcaata tcgactgcga cctgacctgg ggctactgcg acctggccaa caaacccagc
    39181 cacctcgccg acttcgccga agacatggct gagctcaagc agctgggtta tcgccatgaa
    39241 atgcgcctgc tgcaaccgga gcagatgcac gaagtggtcg gctccaaccg ctacgtcggc
    39301 ggcatgatcg acatgggttc cggccacctg cacccgctca acctggccct cggcgaagcc
    39361 gctgcggcgc agagcctggg cgtgcagctg ttcgagaact cggcggttac ccgcatcgac
    39421 tacggcagcg aggtcaaggt gcataccgct cagggcgtgg tgcgcgcggc caccctggtg
    39481 cttggctgca acgcctacct gaatggcctg aacagcaacc tgggcggcaa ggtgctgccc
    39541 gccggcagct atgtgatcgc caccgagccg ctgtccgagg ccgaagcgcg ggcaatcatt
    39601 ccgcagaata tggccctgtg cgaccagcgc gtggccctgg attactaccg cctatccgcc
    39661 gaccgccgcc tgctgttcgg cggtgcctgc cactactcag gtcgcgatcc tgcaaacatc
    39721 gccggctata tgcgaccgaa aatgctggaa gtcttcccgc acctgaaaga tgtgaagatc
    39781 gactaccaat ggggcggcat gatcggcatc ggcgccaacc gcctgccgca gatcggccgc
    39841 ctcaaggaac agcccaacgt gtattacgcc caggcctact cgggccacgg cgtcaacgcc
    39901 acgcacctgg ccggcaagct gctcggtgag gccatcgctg gccaggccag cagcggcttc
    39961 gacctgttcg ccaaggtacc gcacatgacc ttccctggcg gcaaacacct gcgctcgcca
    40021 ttgttagcgc tgggcatgct gtggcatcgg atgaaagagc tggtctgatc gccctattca
    40081 gcaggccaac gaggcctgct gtgaactccc cttacagctt ccagaacggc agaaacgcct
    40141 cgcgccgggc ctgttcacgg ttcaggccga catccttgag ctgttcatcg gtcagcgtca
    40201 gcagcgcccg gcgggttgtc aggcgcagcc agaatgcgtt ccagcgactt acagctggct
    40261 taaccgcacc agcctgagca tacagatgct cgccagtaga ctcctgctgc aactcttctg
    40321 acttcatggt cagcctgaca tcacttaaac cgttcatcgc ctgctcctgc ccgtaagcct
    40381 gattggagat catgatgagc gaggcagaaa aagcatgaca gatgcaaaaa tgtactttta
    40441 tatccataca gatttaagcg cacgagctct gaatgatgga ttttttgtcg atctgtactg
    40501 gcttagcgca ctacatagag tcattgcgag gcaactgtta tgacgctcta tatcaatcta
    40561 gccgaactgc tcggcacccg aattgagcag ggcctttatc gccccggtga tcgcctgccc
    40621 tccgtacgcg ccctgagcct ggagcatggc gtcagcctca gcacagtgca gcaggcctat
    40681 cgccacctgg aagaccaagg cctggccacg cccaggccca agtccggcta ctttgtgccc
    40741 caggcgcgcc accagccggc gctgcccatg gtcagtcgca tggctcagcg tcccgtggat
    40801 gtctcgcagt gggatcaggt gctggagctg atcagccgac cgccggggca ggaagacctg
    40861 ctgcaactcg gccgtggccg gccggatatc gacagtccga cgctaaaacc gctgctgcgc
    40921 tcactgtcgc gcctgagccg ccgccaggac gccaacagcc tgacctacgg ctgcatttac
    40981 ggccacctgg gcatgcgtga acagatctcg cggctaatgc tggactccgg ctgcagcctg
    41041 tcaccccagg agatcatcgt caccacaggc tgccacgagg cgctgtcggc agcgatccgc
    41101 gccatctgcg agcccggcga tatcgttgcg gtggattcgc cgagctttca cggcgtgatg
    41161 caggcgctca agggttttgg catgaaggcc ttggaattgc ctaccgaccc gatcaccgga
    41221 atcagcctgg aagccctcga attggccctg gagcaatggc cgatcaaggc catccaactg
    41281 accccgacct gcaacaatcc gcagggctac attatgcccg acgccaacaa acgcgccctg
    41341 ctggccctgg cacagcgcta tgacgtggcg attatcgaag atgacgtcta cggcgacctg
    41401 gcctacagct acccgcgccc acgcacgatc aaatcctatg acgaggatgg ccgcgtgctg
    41461 ctctgcagct cgttttccaa aaccctggcg cccggcctgc gcatcggctg gattgccccg
    41521 gggcgctacc tggaccgcgt gctgcacatg aaatacatgg gcaccggtag caccgcccag
    41581 ctgccgcagc tggccatcac cgaatacctg caaaccggcc attacgagcc gcacctgcgg
    41641 cgcatgcgcg cgcagtatca gcgcgggcgc gacctgatga ccgactggat cagccgctac
    41701 ttccctgccg gcacgcgggt cagcaggccg cagggcagct ttatgctgtg gcttgaattg
    41761 ccgacaggct tcgacagcca gcgcctgaac cgcgaactgc tgcaacacgc catccagatc
    41821 gcccccggta acatcttttc cgccgccggc aaataccgca attgcctgcg cataaactac
    41881 gccggcaaag ccacaccgga gcttgaacag gcaattcgcc aggtcggcga atgcgtggca
    41941 gcgctgctta gcgaacagca gatcgccgaa acagcggcag acgccatgct cacatcctgg
    42001 cgctagccac ctcgctaaac gcgccgcgct gaaacgccag ccagacaaac actgcagcgc
    42061 caaccgccat cagaatcggc agggcatggc cactgaccca ctggctggcg gcgccggtcg
    42121 acagcggacc gagcaggcaa ccggcgcccc atagcaaggc gatatgcgag ttggcgcgca
    42181 ccagcgcatc gtcgcgatag cgctggccga ccagaatcag cgacagggtg tacaggccgc
    42241 ccgcgctggc gccgaacagc accaacacca cccagatcag cggcgtatgc agcaataacg
    42301 gaatgctcag gctcgacagt aacaacacca ccccgcagca acggaatagc gtttgccgtg
    42361 gtacacgatc ggccatccag ccaatcggca gctgcagggc agcatcgccg accaccaccg
    42421 cactgaccat gaccaacgcc agctgctggg caaagccttc gcgcaccaga tacaccggca
    42481 gcagggtcag gaccatcgcc tcgaaggcgg caaacaacac cactgcccag gcaatcgctg
    42541 gcgaggtgcg gcaaaacgcc agaatgccgc gcccagacgc actctgcgca tcgaccttgg
    42601 gcgcgccatc gcggcccaac agcagcaggc taccgcccac cagcagcgca gcggagaacc
    42661 agaaaccacg gtcactttca gtccccaaca gcgtcagcag cagcggtccg cacagctgac
    42721 tgagagcaaa gccggtgccg tacagcgcga ccagacggcc gcgcagacgg tcctcgacca
    42781 gctgattgat ccagctttcg ccaagcacaa acaccacggt caatgaaatg ccaatcagca
    42841 ggcgcagcag cagccacagc ggatagctcg gcatcacgct aagcagcgcc accgacgccg
    42901 cactggccag taggcacagg cgcaaggttt gcggcgtgcc gaaccagccg gccagatgcc
    42961 cagtcagccg cgcgccgagc agcacaccaa cggccggcat agcggccatc acgccaatgg
    43021 caaaggggcc ataaccccag gcctcgaggc gaaacgacac cagcggcagc gtcacgccca
    43081 gcgacagccc aacgatcacc accgccgcac tgacggcgaa ataggtcatc cagttcaatc
    43141 agccaactcc caccccatgg agccaatcac tcacggccct gccgcgcaaa aaacaacggc
    43201 cgagctcctg ttcaggaacc cggccgcggg tttggctcat atagagccgg cccaacacgg
    43261 gccgacctac agcagtgcta tttagagttt gatccaggtc gccttaatct cggtgtactt
    43321 gtcgaaggcg tgcagcgact tgtcgcggcc gttgccggac tgcttgaagc cgccaaacgg
    43381 cgcggtcatg tcaccgccgt cgtactggtt gacccacatg ctgcccacac gcagcgcctt
    43441 ggccgccaga tgcgccttgg acaggttgct ggtccagatc gccgccgcca aaccgtagat
    43501 gctgtcgttg gcgatcttga tcgcctcctc ggtgctgtcg aagggaataa cggtcaacac
    43561 cgggccgaag atctcttcct gggcaatgcg catggcgttg tgcgcgtcat cgaagatggt
    43621 cggctcaacg tactggccgc ctgtttcttc cagagtgcgc ttaccgccgc agagcagctt
    43681 ggcctgatcc ttatgaccgg cgtcgatata accgagcacc gcattcaggt gaccggcatc
    43741 gaccagcgcg ccgaccttgc tgtccggatc cagggcgtga ccggctttcc agccctgcat
    43801 ggcttccagg accatgggca taaatgtgtc tttgatcgaa cgctccacca gcagacgcga
    43861 accagcggtg cacatctcgc cctggttgaa gcagatcgcc gcagcagcag cctcggccgc
    43921 agccttgagg tccggcgcat cggcgaagac gatattcgcg ctcttgccgc cggcttccag
    43981 ccaaacgcgc ttcatgttgg attcgccagc gtagatcatc agctgcttgg cgatcttggt
    44041 cgaaccggtg aacaccagag tgtcgacatc catatgcagc gccagcgcct tgccgacggt
    44101 gtggccgtaa cctggcagca cattgagcac gccggccggg atgccggcgt ccagggccag
    44161 ctgggccatg cgcagggcgg tcagcggcga tttctccgag ggtttgacga tcaccgagtt
    44221 gcccgtggcc agggctggac cgagcttcca gcaggtcatg atcatcggga agttccacgg
    44281 cacgatggcc gccactacac ccactggctc gcgagtcacc aggcccagtt cgtcgtgcgg
    44341 ggtggcggcc acttcgtcat aaattttgtc gaccgcttcg ccgctccagc gaatcgcccg
    44401 cgaggctgcc ggtacatcaa cgctcaaggc atcgctgatt ggcttgccca tatccagggt
    44461 ttccagcagc gccagctctt cagcgtgctg atccagcagg tcggcaaagc ggatcatcac
    44521 gcgcttgcgc tgcgccgggg ccatacgcga ccagacgccg gactcgaagg tggcgcgggc
    44581 gtcacttacg gccagctcgg catcagccag atcacagctg gccaccaggc cgagcaggcg
    44641 accgtcaacc gggctgatgc attcgaacgt ggccccggag acagcggcgc ggtactcgcc
    44701 atgaacaaag gcacggcctt cgatcttcag gttcttggcg cgctgctccc agtcggcacg
    44761 agtcaggcta gtcattgcta catcctctat cgctagtaaa gcgccgcagg gtggccgcac
    44821 gcgctgtcaa ttattctgca tgggcctcgc gacccaatcc tgccggcaac actaaaccag
    44881 aggcttgcac acttccaata ttttttacaa aaactggcaa aaccgccttg ttatgtgtgc
    44941 tttattaaac atagactgcc gaaatcgcca cccaaccaat aacaagcctg gccttaacag
    45001 gcgctcaatc cctaccggac atgcttatga atatcgaaaa gatcgtcgac ttcgccaccg
    45061 tcaccaccac gcccgaacat taccgcccgg cagcagaaaa ggtgctcaag ggcgaccccg
    45121 agcaaagcgt gcgcaaccat tacggcagcc catgcggtca gttcaatgcg ggaatctggg
    45181 aaggcgccgt cggccactgg accatcaact acaccgaaca cgagtactgc gagatcctgc
    45241 aaggcgtctc ggtgctgcgc gacaaggacg gcaatgccaa gaccctgcgc gcgggcgacc
    45301 gcttcgtcat cccggccggc ttttccggca cctgggaagt gctggagccg tgccgcaagg
    45361 tctatgtgat cttcgagcaa gccagcaact gatagctatt catgcgtccc cccttcgccc
    45421 gccttgcgcg ggctttttat cgcctgtcat cctgacggcc accttctagg tttaccagac
    45481 caaatcgcag gcaagaaaaa gcccgctata aaagcgggct ttttcggcaa ggtgccagat
    45541 caattacttg atcttgcctt ccttgtagat cacgtgctta cgcacaaccg gatcgaattt
    45601 tttaatttcg attttgtccg gagtggtgcg cttgtttttg tcggtagtgt agaaatgacc
    45661 agtaccggca ctcgagatca aacggatcaa ttcacgcatg actttctcct taaacctttt
    45721 cgccgcgagc gcgcagctcg gacagaacga cgtcaatacc acgcttgtcg atgcaacgca
    45781 tgcctttggc agaaacgcgc agacgcacga aacggttctc agactcgacc cagaagcggt
    45841 gatgctgcag gttcggcagg aaacgacgac gggttttgtt gttagcgtgg gaaatgttat
    45901 tcccggttac cggaccctta ccggtaactt gatagactgt cgacatacat cagccctcta
    45961 aaaccacatg cccaacccgg catgggttgg ccgcttaaac tcaaagtcat ttggcgctcg
    46021 gcgccacgtt ttttcagggt cttaccggcc acgcctataa agcggaggaa ccgggcccct
    46081 agaaaagagc gcgcctttat atcagaaaca cccccttgca gcaagaacag tggcgctttc
    46141 tagtgcaccg ctgccgccgt caaagcgcct atccccatgc ggacggggca taaccaggcc
    46201 ccgaccattc gtcgcacgct tacagccaac cgtactcggc catggacagg ggctcgccgt
    46261 caccgacgat aaagtgatca agcacgcgca catccaccaa ctccagagcc tctttcaggc
    46321 gccgggtcag cactcgatcg gcctgactcg gctcagccac cccggacgga tggttgtggg
    46381 tcaggatcaa cgcggcggcg ttatgcgcca gggcacgctt gaccacctgg cgcgggtaaa
    46441 cgctggcgcc atcgatgctg ccgtgaaaca gcacctcgaa cgccagcacc cggtgcttgg
    46501 catcgagaaa caggcaggcg aagacttcat ggggttcgtg gcgcaaacgc gcct
//
LOCUS       DASRUM010000030        15666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2356713, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000030 DASRUM010000000
VERSION     DASRUM010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 15666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15666
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2356713"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..548
                     /locus_tag="VFF22_02390"
     CDS             84..548
                     /locus_tag="VFF22_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HZX15704.1"
                     /translation="MLFGAILVLSWLILLIRYPSKALPISLGALIGLGLVASWVLWQE
                     SRENRHLAHLELRLAYAPDSCPADRPLALNLKNGSEAALLELHWQIAAYRPGDSINLA
                     ERLYETPRYSGPGELLPGTDWHTCLPLPTLRSGYRASTLEFRAERLQGSFSR"
     gene            578..1408
                     /locus_tag="VFF22_02395"
     CDS             578..1408
                     /locus_tag="VFF22_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX15705.1"
                     /translation="MNQPSVLITGCSSGIGRALAEVFQQAGYQVWATARKAEDLAALN
                     AAGFHAVQLDVNDGLALGQLAERLKAEIGGLDVLINNAGYGAMGPLLDGGVEAMRKQF
                     ETNVFSLIGVTRALFPLLRQNKGLVVNIGSVSGVLVTPFAGAYCASKAAVHALTDALR
                     LELAPFAIEVMEVQPGAIASSFGANASQQAEQLIDEASPWWPIRDGIRARANASQDNP
                     TPTSHFARNLLAAVQSKSRPNLLRLGNGSRSMPLLAALVPKRLMESILKKRFGLNRSL
                     "
     gene            1428..1808
                     /locus_tag="VFF22_02400"
     CDS             1428..1808
                     /locus_tag="VFF22_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15706.1"
                     /translation="MNPPNTDNVSTTHLAIGGVVAVMVLNLLVRSFVKLGGVLSTLLV
                     AALVAGGMALWFHLQHRRRPLPSEHRKLLALYGGILALLYLGLLGLMWLKDEPGPMGL
                     FIFGLHYFCYPLLAWLALAIPKDS"
     gene            1910..3847
                     /locus_tag="VFF22_02405"
     CDS             1910..3847
                     /locus_tag="VFF22_02405"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZX15707.1"
                     /translation="MKIKEIELNNIKSFDGHITCAFDDLTSIFSISGKNGAGKSTILK
                     SAYLIQKAYFSKLLGDSHWIEFEHEVKRFLNSYDSIIRLVLCDKDEDCILTLKYTNKT
                     IDLEYSNEKFIKKYWNLTSPENLILYIDASKGFSEDTLKFNELNISDNSRNDLAIEAV
                     LRPGLLFSGIYRQLVKDHIHGRLIPSKPDRLLYFRVASKMFTSLIPNVELRNFSGKHK
                     PGEFVLLGKANTDKRKPLYDVREFSSGEKALLSTLAFLCISKSVCALFIDEPENHFHE
                     SLLLEFVSLLHALCESGGLLGWATKENEAEGQINLKEEWLEKEYKDHRLNQVVLSTHS
                     KTLIYKIFTVGKNFTVSSSIKELNYDDAENELRGLGLSSIYSKVLLVEGTGDHESLEY
                     ILKGKSVTIKPLGGSKEVIETFKKLVVLRKHIREMEFVFLVDSDNKPEEYFEKIRALD
                     EDFYDKTFIKLSKHEFENYLLDADIFEHVINQYIEISGESDKKITKEEINQQFSELAK
                     KSLPQVYKKETSLVLNHKIDSYFSRKIWGNKDFVWDSKEKILEQIINQALDETSIQAL
                     ANDLKESVTSVFDAYTQNADTTLIDRCDGKQVFGLASAFFSKYTGIDNKVFKKAIYRK
                     AERTEGSQLTGLIREILNRFS"
     gene            complement(4072..4272)
                     /locus_tag="VFF22_02410"
                     /pseudo
     CDS             complement(4072..4272)
                     /locus_tag="VFF22_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4256..4531
                     /locus_tag="VFF22_02415"
     CDS             4256..4531
                     /locus_tag="VFF22_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15708.1"
                     /translation="MSDTFVKLGGLFTTVLIAALVAAGMTLQGERRRLLALYGGILAL
                     LYLGLLAMMALQDDPNPMGLLIVGLHYFCYPLMAWLFLAKGYGKPSQ"
     gene            complement(4632..5588)
                     /locus_tag="VFF22_02420"
     CDS             complement(4632..5588)
                     /locus_tag="VFF22_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HZX15709.1"
                     /translation="MHIVIPDDYQNVISSLDCFAQLGGHKVSLYHDVQKDPRVLAQRF
                     ADADALVLTRERCVIDEALLAQLPRLKLISQTGRVGPHLDLQACSRHGVAVMEGRGSP
                     IAAAELTWALMLNARRQLRTAIDGLYAGHWQVNLGERLCGQTLGIWGYGKIGQRLARY
                     AQAFDMPVLVWGSEASRLAAQRDGHRAAESRQAFFSEADVLSLHLRLVDSTRHAVTLD
                     DLLWMKPGALLVNTSRAELIAPGALLTAIIQGRPGFAALDVFEDEPLLDPANPLLNHP
                     RILCTPHLGYVERHSYELYFGDAFANLLAYFNGHKCTLVNPV"
     gene            complement(5620..7131)
                     /locus_tag="VFF22_02425"
     CDS             complement(5620..7131)
                     /locus_tag="VFF22_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HZX15710.1"
                     /translation="MDTLYYLGIGFGVALTPYNLLTAFCGTLIGTIVGLLPGLGPVNG
                     VALLIPIAFALGLPPETALILLAAVYLGCEYGGRISSILLNIPGEASAVMTTLDGYPL
                     ARQGKAGVALSISAWSSFIGGTIATVGVVLFAPLLAKWAVAFGPAEYFVLMVFAITCL
                     AGMADNKPLKTAVAALIGLFLSCVGIDANSGVYRFTFGSLGLADGIQFVVLVLGLFSV
                     SEILVLLERTHHGQKAVKASGRMLFNMKEGASVFATNMRSGVVGFVLGVLPGAGATLA
                     SAVAYMSEKRLATKDNQFGKGDLRGLAAPETANSAAACGSMVPMLTLGVPGSGTTAVM
                     LGALTLYNITPGPMLFQNQPDIVWGLIASLFVANVMLLIMNVPMVRVFTRILAVPTWA
                     LVPAIAIITSIGVYAVHSTTFDLFLMIGIGVCGYILRKLDYSLSAVLLGFILGGMMEE
                     NLRRALSLSSGELGILWGSPITIAVWALVAVMVALPIWRAWRGRKPAAVALTE"
     gene            complement(7135..7590)
                     /locus_tag="VFF22_02430"
     CDS             complement(7135..7590)
                     /locus_tag="VFF22_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HZX15711.1"
                     /translation="MHDRVFAGSNLLLCLLLAFLAWAYQAPFTYEPVGPRAFPLLLLL
                     LITVALLYLLCKPLSRQKIQEEPALDRHVSRKIVLCVAALLVYSALFEVMGFILSSVL
                     FAVGMARLYGASWLQSGLGGSVIAVGLYLLFDYGLDVPLPLGMLAALGN"
     gene            complement(7640..8620)
                     /locus_tag="VFF22_02435"
     CDS             complement(7640..8620)
                     /locus_tag="VFF22_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HZX15712.1"
                     /translation="MRNAKLSKILPLTCALLLGSFNAAAGEPARPECIAPTKPGGGFD
                     LTCKLAQAGLKDEELLKAPMRVTYMPGGIGAVAYNAIAANRRAEAGTIVAFSGASLLN
                     LALGKYGRYDEDAVQWLTTIGTDYGTLAVSEDSPYKTLDDVVAALKKDPKSISVGGGG
                     SIGGQGWVKIALLAKAAGVNPRDLRYAAFEGGAEHYMALMGKHVDLVTGSASEIRAQK
                     ANKIRTLAIFSEERLPGELAEIPTAREQGYDIQWPLIRGYFVGPDVSPEAVNWWRETF
                     ATLQASPEFQKYLSDRDVLPLNVTGAELNALVKQQVAQYRDLGREFGLVE"
     gene            complement(8666..9865)
                     /locus_tag="VFF22_02440"
     CDS             complement(8666..9865)
                     /locus_tag="VFF22_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015802 - basic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family porin"
                     /protein_id="HZX15713.1"
                     /translation="MSLPAAHAAGFIEDSKASLELRNFYLNRDLHDGAGQNKREEWAQ
                     GFILNIESGYTQGTVGFGLDALGMLGVKLDSSPDRSGTDLLPRDSDNRAEDDYSKLGL
                     TAKARFAESQLKVGTLLPKMPYLQYSNSRLLPQTFEGWQLQSKDIDQLTATFGEVSRV
                     VQRSVSGSEKLRLNNKNDRFGASPESDELRFAGMDFALSAQTLASYHYAELSDVYRQH
                     FVGLQHTAALGAGKLKSDLRYFNNSEAGQERVGAIDNQAYNAMFTYSLSGHALGVAYQ
                     KMSGDEAFTYVDGATPYLTNFLQINDFAGPNERSWQLRYDYDFANLGIPGLTFMTRYV
                     SGDQIERSNGQQGDEWERNTDLAYTFSQGSLKNLKVTWRNATYRSNFSRGLDENRLIL
                     SYVQPLW"
     gene            complement(9959..11041)
                     /locus_tag="VFF22_02445"
     CDS             complement(9959..11041)
                     /locus_tag="VFF22_02445"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HZX15714.1"
                     /translation="MQAIPCVLMRGGTSRGPVFLANHLPSNPALRDEVLLKLMGSGHE
                     LEIDGIGGGSPQTSKVAIVSASSHPDADVDYLFVQVMVAQRRVDTAPNCGNMLCAIGP
                     FAIEHGLVNAAAPVTRVRIRNVNTNTLIDSEVLTPNGKVRYEGDTSIDGVPGTAAPIK
                     LTFLDAAGAKTGRLLPTGQVRDQFDGVVVTCIDMAMPMVLMHAEALGKTGYETPAELD
                     SDAELLQRLESIRLQAGRAMGLGDVSQMVIPKPVLLAPARQGGTLSTRYFMPHNCHRS
                     LAATGAIGLASACALPGSVAHDLAPISGAALVSLEHPGGQIAVSLEPGDAENPEKIRA
                     SLIRTARRLFAGDVYVASPRRSAAEH"
     gene            11264..12181
                     /locus_tag="VFF22_02450"
     CDS             11264..12181
                     /locus_tag="VFF22_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX15715.1"
                     /translation="MNYELQDLRAFVKIAEFGNFHEAANAIHLSQPALTRRMQKLEEG
                     LGTQLLDRTTRRVSLTAVGRDFLPKARRLLDDFERSILGIRELAERQSGQVTLACIPT
                     AAFYFLPTVIREFNLQYPKIRIRILDLSAHEGLEAVLRGEADFGINMLSGQSAEIDFT
                     PLVEEPFVLACRRDHPLAAQPQVAWRELSDYRLIGVGRLSGNRVLLDHGLAHLDWRPS
                     WFYEVQHLSTSLGMVEAGLGVAALPSLAMPAEDHPILVQRPLVDPVISRTLGLVRRHG
                     SSLSPAAEQFVDTLLRQWPSYRSLGKDKQ"
     gene            12490..13350
                     /locus_tag="VFF22_02455"
     CDS             12490..13350
                     /locus_tag="VFF22_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003289997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZX15716.1"
                     /translation="MSYPRLVSMTALALIAFAANSLLCRAALADGSIDAASFTSIRLI
                     AGAAVLWLIVRIRAGSAVGKGNWLSALALFAYAAGFSFAYIELSTATGALLLFGAVQS
                     TMIGYGLYRGERLRVPQLGGLLLAIGGLVGLLLPGISAPPPLASALMLSAGVAWGIYS
                     LRGKGAGDPLQVTAGNFLRAVPIALILSTATLGDITLQSAAGIAYAIASGALASGLGY
                     AVWYSALPALKATTAASVQLSVPVIAALGGVVLLAEPLSLRLLLASLAILGGIALVIL
                     TGQRTKPASR"
     gene            complement(13557..15554)
                     /locus_tag="VFF22_02460"
     CDS             complement(13557..15554)
                     /locus_tag="VFF22_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012314720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HZX15717.1"
                     /translation="MSLPKHHLELLSPARDTAIAKEAILHGADAVYIGGPGFGARHNA
                     SNSVADIAELVQFAHLFHARVFVTLNTILHEDELEPARQMIWQLYEAGVDALIVQDMG
                     VMEMDIPPIEIHASTQCDIRTLEKARFLDGAGFSQLVLARELNLEEIGNICRNVDSAV
                     EFFIHGALCVAFSGQCNISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVAFDKHLLS
                     MKDNNQTDNLIYLVDAGVRSFKIEGRYKDMSYVKNITAHYRRELDAILEQRPHLERAS
                     SGRTEHFFTPDTDKTFHRGSTDYFVTDRKVDIGAFDSPTFTGLTVGVVEKVNKRDLIA
                     VTDTPLSNGDGLNVLIKREVVGFRANIAELKAEFEEDGEKRYRYRVEPNEMPEAMHRV
                     RPLQPLHRNLDHNWQQALQKTSSERRIAVSWQLSLRGEQLNVSVASDDGISASVSLAG
                     PFAAAKDAEQAREQLADGLSKLGTTIYYSAGAQIDCEVVPFIPGSQLKALRREAIEAL
                     TQARVAAHPRGGRKAVSVPAPVYPESHLTFLANVYNDKARTFYKRYGVQLIDAAYEAH
                     EESGEVPVMITKHCLRFSFNLCPKQAKGVTGVRTKVSDMQLIHQDEVLTLKFDCKPCE
                     MHIIGKMKGHILHQPQPGSAQSKGGAVGYITPEDLLKTIKR"
BASE COUNT         3368 a         4707 c         4508 g         3083 t
ORIGIN      
        1 ggctggcggg ccaaagcatg tacccagccg cccggccttg tgtaggatga acgtctgttt
       61 atcgaccaag gccatctatt cccatgctat tcggcgcaat cctggtactg agctggctga
      121 tcctgttgat ccgctacccg agcaaggccc tgccgatttc cctcggcgcg ctgatcggcc
      181 taggcctggt cgccagttgg gtgctctggc aggaaagccg cgagaaccgc cacctggccc
      241 acctcgaatt acgcctcgcc tacgccccgg acagctgccc ggcggaccgc ccgctggccc
      301 tgaatctgaa gaacggcagc gaagccgcac tgcttgagct gcattggcag atcgccgcct
      361 accgccccgg cgacagcatc aacctggccg agcgcctgta tgaaacccca cgttacagcg
      421 gccccggcga attgctgccc ggcactgact ggcacacctg cctgccgcta cccaccctgc
      481 gcagcggcta ccgcgccagt actctggagt ttcgcgcaga gcgtttgcaa ggcagtttca
      541 gccgctaagc cccatccata acaacaagga agctcgaatg aaccagccca gcgtactgat
      601 caccggatgt tccagcggta ttggccgcgc cctggccgag gtgttccagc aggccggtta
      661 tcaggtctgg gccaccgcgc gcaaagccga ggacctggcc gcattgaacg ccgccggttt
      721 tcacgccgtg caattggacg tcaacgatgg cctggccctg ggccagttgg ccgaacgcct
      781 gaaagctgag attggcggcc tcgatgtgct gatcaacaac gccggctacg gcgccatggg
      841 cccgctgctc gatggcggcg ttgaagccat gcgcaagcaa ttcgaaacta atgtgttctc
      901 gctgatcggc gtcacccgcg cactgttccc gctgctgcgg cagaacaagg gcctggtggt
      961 aaatatcggc agcgtttccg gcgtgctggt caccccgttc gccggcgcct actgcgcctc
     1021 caaggccgcc gtgcatgcgc tgaccgatgc cctgcgcctg gagctcgcac ccttcgctat
     1081 cgaggtgatg gaagtacagc ccggcgcgat tgcctccagc ttcggcgcca atgccagcca
     1141 gcaagccgaa cagctgatcg atgaagcctc gccctggtgg ccgatccgcg acggcatccg
     1201 cgcccgcgcc aacgcctcgc aggacaaccc cacccccacc agccacttcg cccgcaacct
     1261 gctggccgcc gtacaaagca agtcccggcc aaacctgctg cgcctgggca atggcagccg
     1321 cagcatgccg ctgctggccg cactggtgcc taagcgcttg atggaaagca ttctgaaaaa
     1381 acgcttcggc ctaaaccgca gcctctaaat caccgggagc gcgcacgatg aacccgccca
     1441 acactgataa cgtctcgaca acccacctgg ccattggcgg cgtggtggcg gtcatggtgc
     1501 tcaacctgct ggtgcgcagc ttcgtcaaac tcggcggcgt actcagcacc ctgctggtgg
     1561 ccgctttagt agccggtggc atggccctgt ggtttcactt acaacaccgc cgccgcccat
     1621 tgcccagcga acaccgcaag ctactggccc tctacggcgg catcctcgcc ctgctctacc
     1681 tgggcctact cggcctgatg tggctcaaag acgaacccgg cccaatggga ctgtttatct
     1741 ttggcctgca ttacttttgt tatccgctgc tggcgtggtt ggcacttgct attccgaagg
     1801 actcataaag ctcggcaata ccgttatatt aggaagatat acaaatacaa aacataagaa
     1861 ttcgactcag taacaccacc catacaaaaa ccaccaagga tgggcaaaat tgaaaatcaa
     1921 agaaattgaa cttaataata taaaatcctt tgacggacac atcacctgcg ccttcgatga
     1981 cctcacatca attttcagca tttctggaaa aaatggtgct ggcaagtcaa ccatcttgaa
     2041 gtctgcctac ctaattcaaa aagcatattt ttcgaaatta ctaggcgact cacactggat
     2101 cgaattcgag catgaggtaa aacgcttctt aaactcatac gactcaatta taaggcttgt
     2161 cctctgtgac aaagacgaag attgcatact gacacttaaa tacacaaaca aaaccattga
     2221 ccttgagtac agcaacgaaa aatttattaa aaagtactgg aaccttacat caccagaaaa
     2281 cttgatttta tacatcgatg caagcaaagg attctctgaa gacaccttaa aatttaacga
     2341 acttaacatt tccgacaaca gcaggaacga tctagcgata gaggccgtac taaggccagg
     2401 gcttttgttt tctgggatat atcgacaact agttaaagac cacatccacg gaagattaat
     2461 accaagcaaa cccgacaggc tactctactt cagagtcgca tcaaaaatgt ttacatcgtt
     2521 aatcccaaac gttgaactaa gaaattttag tggcaaacac aaacctggag agttcgttct
     2581 actagggaaa gcaaacacag acaaacgaaa acccttatac gatgttagag aatttagttc
     2641 cggcgaaaaa gcactactca gcaccctagc ctttctatgt atatctaaat cagtttgcgc
     2701 attatttatt gatgagccag aaaaccactt ccacgaaagc ttactgttag agttcgtaag
     2761 cctattgcat gcattatgcg aaagtggagg actactgggc tgggccacaa aagaaaacga
     2821 agccgaaggc caaataaatc taaaagaaga gtggctggaa aaagaatata aagaccacag
     2881 actaaatcaa gtagttttat ccacacattc caaaaccctt atttataaaa tattcaccgt
     2941 tggtaaaaat ttcacagtct ccagctctat caaagagcta aattatgacg acgccgagaa
     3001 cgaactcagg ggattgggcc tgagttcaat atatagcaaa gtactactag tggaaggcac
     3061 gggtgatcac gagtcgcttg aatatatatt aaaagggaag agtgtaacaa tcaaaccgct
     3121 aggtggaagc aaagaagtta tagagacatt caagaaatta gtagttcttc gcaagcacat
     3181 tagagaaatg gagtttgtct ttcttgtaga ctccgacaac aaacctgaag agtatttcga
     3241 aaaaatcaga gcacttgacg aagattttta cgacaaaaca tttataaaac tatctaaaca
     3301 tgagtttgaa aactacctac tagacgcaga catatttgaa cacgtcataa accaatacat
     3361 agaaatatct ggcgaaagtg acaaaaaaat caccaaagaa gaaatcaatc agcaattttc
     3421 agagcttgct aaaaaatcac tccctcaagt ttataaaaaa gaaacatctc ttgtattaaa
     3481 tcacaaaata gactcttact tttcaaggaa aatatgggga aacaaagact ttgtatggga
     3541 tagcaaagaa aaaatattag aacaaataat taaccaagcc ttggatgaga caagcattca
     3601 agcactagca aatgacttaa aagagtctgt aacttctgtg tttgatgctt acacccagaa
     3661 cgctgacacc acactaatag accgctgcga cggaaagcaa gtgttcggcc ttgcttctgc
     3721 atttttctca aaatacaccg gtattgataa taaagtattt aaaaaagcaa tttaccgtaa
     3781 agcagaaaga acagaaggca gtcaattaac cggattaatc cgcgaaatac taaatcgctt
     3841 ctcataaaac ttaagagtta tttacacgac aacttgcgat cgcgtcgact ccgaaaaaac
     3901 acgtcgaaat agtgttttct tcgatatacg agcccgcagg atgggttgag cagtgcgata
     3961 cacatcagca atcgcaccct caacaccgtc agccgtccta gcgagaagtt tgctggttat
     4021 cgctgcgttc acctgcgcag cccgccccat cctacaattc gcctttcggc tctagcgctt
     4081 gatggttttt agcagatctt ccggggtgat atagcccacc gcaccgcttt gagtctgggc
     4141 gctgcccggt tgcgggtggt gaaggatatg gcccttcatc ttgccgatga tgtgcatctc
     4201 gcagggtttg cagtcgaact tcagggttag cacttcgtcc tgatggatca gctgcatgtc
     4261 cgacaccttc gtcaaactcg gcgggctgtt taccacggtg cttatcgccg cactggtggc
     4321 cgcaggcatg accctgcaag gcgaacgccg ccgtctgctc gcactctacg gcggcatcct
     4381 ggcactgctc tatctggggt tattggcaat gatggcgctg caagacgacc ccaaccccat
     4441 ggggttgctg atcgttgggc tgcattattt ttgttatccg ctgatggcct ggctgtttct
     4501 cgccaagggc tatggcaagc ccagtcagtg accttattgc cgagaggggc ttcaacctat
     4561 caagtacgac ggtcgcccct accatcccga cagcaacccg cttgtaatgg cgcgcaaccc
     4621 acgaatccgc gctacacagg gttgaccaag gtgcacttgt ggccattgaa gtacgccaac
     4681 aagttggcga aggcatcgcc gaaatacagc tcgtagctgt gccgctcgac atagccgaga
     4741 tgcggggtgc agagaattct ggggtggttc agcaacgggt tcgccgggtc cagcagcggc
     4801 tcgtcctcga atacatcgag ggcagcaaag cctggccgac cttggattat ggccgtcagc
     4861 agggcccctg gggcaatcag ctcggcgcgg ctggtattga ccagcaaggc accgggtttc
     4921 atccacagca gatcgtccag ggttaccgcg tggcgcgtgc tgtcgaccag gcgcagatgc
     4981 aggctgagca catcggcctc gctgaagaac gcctggcgcg attcggccgc acgatggcca
     5041 tcacgctgcg ccgccagacg tgaagcctcg ctgccccaca ccagcaccgg catgtcgaaa
     5101 gcctgggcat aacgcgccaa gcgctggccg atcttgccat agccccagat gcccagcgtc
     5161 tgcccgcata aacgctcgcc gagattgacc tgccagtgcc cggcgtagag cccatctatc
     5221 gccgtgcgca gttgccggcg ggcattgagc atcagggccc aggtcagttc ggcggcagca
     5281 atcggcgatc cccgcccttc catcaccgcc acaccatgac gactacaggc ttgcagatcc
     5341 aggtgagggc caaccctgcc ggtctggctg atcaacttaa gccggggcaa ctgcgccagc
     5401 agggcttcat cgatcacgca gcgctcgcgg gtcagtacca gcgcatcggc atcggcgaag
     5461 cgttgcgcca ataccctggg atccttctgt acatcgtgat acaggctgac cttgtgcccg
     5521 cccaactgcg cgaagcagtc gagcgagctg atgacattct ggtagtcatc gggaatcacg
     5581 atatgcatgc cacgcctccc acgccttggc gctgctctat cactcggtaa gcgcaacagc
     5641 tgccggtttg cgcccgcgcc aggcacgcca tatcggcagg gccaccatca ccgccaccag
     5701 cgcccagacg gcgatggtaa tcgggctacc ccagaggatt cccaactcgc cgctggacaa
     5761 cgacagcgcc cggcgcaggt tctcttccat catgccgccg aggataaagc cgagcagcac
     5821 cgccgacagc gaatagtcga gtttacgcag gatatagccg cacacgccga tgccgatcat
     5881 caggaacaga tcaaaggtgg tcgagtgcac cgcatacacg ccaatcgagg tgatgatggc
     5941 aatcgccggc accagcgccc aggtcggcac cgcgaggatg cgggtgaaca ctcgcaccat
     6001 cggcacgttc atgatcagca gcatgacgtt ggccacgaac agcgaagcga tcaggcccca
     6061 gacgatgtcc ggctggttct ggaacagcat cggcccgggg gtgatgttgt acagcgtcag
     6121 tgcgccgagc atcaccgcgg tggtacccga gcccggcacg ccgagggtca gcatcggcac
     6181 catggagccg caggccgcgg cgctgttggc ggtttccggt gcggccaggc cgcgcaggtc
     6241 gcccttgccg aactggttgt ccttggtcgc cagacgtttc tccgacatat aggccaccgc
     6301 gctggccagg gtcgcaccgg cccccggcaa tacgccgagc acgaagccga ccacgccgct
     6361 gcgcatattg gtggcgaata ccgaggcccc ttccttcatg ttgaacaaca tgcgcccgga
     6421 agccttcacc gccttctgcc cgtggtgggt acgttcgagc aggacgagaa tttcactgac
     6481 gctgaacaga ccaagcacca gcaccacaaa ctggatgcca tcggccaggc ccaggctgcc
     6541 gaaggtgaaa cggtagacgc cgctgttggc atcgatgccg acgcaggata ggaacaggcc
     6601 aatcagcgcc gccacggcgg tcttcaacgg tttgttgtcc gccatgccgg ccaggcaggt
     6661 gatggcgaag accatcagca cgaaatactc ggccgggccg aaggccaccg cccacttggc
     6721 caacaacggg gcgaacagca ccacgccgac ggtggcgatg gtgccgccga taaacgaact
     6781 ccaggccgag atcgacagcg ccacacccgc cttgccctga cgcgccaggg gatagccgtc
     6841 gagggtggtc atcaccgccg aggcttcgcc gggaatgttc agcaggatcg aggagatccg
     6901 cccgccgtac tcgcagccca ggtacaccgc ggcgagcaga atcaaggcgg tttccggcgg
     6961 caggccgagg gcaaaggcga tcgggatcag cagggccaca ccgttcaccg ggccgaggcc
     7021 gggcagcaga ccgacgatgg tgccgatcag cgtgccgcag aatgccgtca gcaggttgta
     7081 gggggtcagg gcaacgccga agccgatgcc caggtaatag agggtatcca taagtcagtt
     7141 ccccagtgcg gcgagcatgc ccaagggcag aggcacatcg aggccgtagt cgaataacag
     7201 gtaaaggccc acggcgatca cgctgccacc gaggccactt tgcaaccagg aggcaccgta
     7261 caaacgggcc atgccgacgg cgaacaagac actgctgagg ataaaaccca tgacttcaaa
     7321 cagcgccgag tagaccagca gcgcagcgac gcacaggacg atcttgcggc tcacatgacg
     7381 gtccagtgca ggctcttcct gaatcttttg ccgcgacaag ggtttgcaca gcaggtacag
     7441 cagggccacc gtgatcagca gcaacagcag cagcgggaag gcccgcgggc ccaccggctc
     7501 gtaggtaaaa ggggcttgat aggcccaggc cagaaatgcc agaagcaggc aaagcagcag
     7561 gttggaaccg gcaaaaactc gatcgtgcat aagggtgctc catggctgag ccgcatagcg
     7621 cggctcagcc aaagtgggat tactcgacca ggccgaactc gcgacctaga tcccggtatt
     7681 gcgccacttg ttgcttgacc agagcattca gctcagcgcc ggtcacgttc aacggcagca
     7741 cgtcacgatc gctcaggtat ttctggaact ctggcgaggc ttgcaacgtg gcgaaggttt
     7801 cacgccacca gttcacggct tcggggctga catcggggcc gacaaagtag ccgcggatca
     7861 atggccactg aatgtcgtaa ccctgctcgc gggcggtggg gatttcggcc agttcgcccg
     7921 gcaggcgctc ttcgctgaag atcgccaggg tgcggatctt gttggccttc tgcgcgcgga
     7981 tttcgctggc actgccggtg accagatcca cgtgcttgcc catcagcgcc atataatgct
     8041 cggccccacc ctcgaaggcg gcgtaacgca agtcacgcgg gttaacgcca gcagccttgg
     8101 ccagcagggc gatcttcacc cagccctggc cgccgatgct gccgccgcca ccgaccgaga
     8161 tgcttttcgg gtctttcttc agtgcagcga ccacatcgtc gagggtcttg tagggcgagt
     8221 cttcgctgac ggccagggtg ccgtagtcgg tacctatggt ggtcagccac tgcaccgcgt
     8281 cttcgtcata gcgaccatat ttgcccagtg ccagattgag cagcgaggcc ccggagaagg
     8341 cgacgatggt gcccgcctcg gcacgccgat tggccgcgat ggcgttgtag gccaccgcgc
     8401 caataccacc aggcatatag gtaacgcgca tcggagcctt gagcagttct tcgtccttga
     8461 ggccggcctg ggccagtttg caggtcaggt cgaaaccacc gccgggcttg gtgggtgcaa
     8521 tgcattcggg gcgtgcaggt tcgccggccg ccgcattgaa gctgcccagc agcaaggcac
     8581 aggtgagggg caggattttg ctgagtttgg cgttacgcat gatgttgtcc tcttattaga
     8641 tttgtcggct atggctatgc actgcttacc aaagtggctg cacgtagctc aggatcaaac
     8701 ggttttcatc cagccctcgg ctgaagttgg agcggtaggt cgcgttgcgc caggtaacct
     8761 tgaggttctt caagctgccc tggctgaagg tgtaggccag gtcggtattg cgctcccatt
     8821 catcgccctg ttggccattg ctgcgttcga tctgatcgcc actcacatag cgagtcataa
     8881 aggtcaggcc cggaatcccc agattggcga aatcgtagtc gtaacgcagc tgccaggaac
     8941 gctcattggg cccggcaaaa tcattgatct gcaggaagtt ggtcaggtag ggcgtcgcgc
     9001 cgtcgacata ggtgaacgcc tcatcgccgc tcatcttctg ataggccacg cccagggcgt
     9061 gccccgagag gctataggtg aacatggcgt tgtacgcctg gttgtcgatc gccccgacgc
     9121 gctcctgccc cgcctcgcta ttgttgaaat aacgcagatc gctcttcaac ttgccggcac
     9181 ccagcgcggc ggtgtgttgc agccccacaa agtgctgacg ataaacatcg ctgagctcgg
     9241 catagtgata gctggccagc gtctgcgcgc tcagcgcgaa atccataccg gcgaaacgca
     9301 gctcatcgct ttccggactg gccccgaagc gatcgttctt gttgttcaga cgcaatttct
     9361 cggagccgga cacgctgcgc tgcacgacgc gggacacctc gccgaaggtt gccgtcagct
     9421 ggtcgatgtc cttgctctgc agctgccagc cctcgaacgt ctgcggcagc aggcggctgt
     9481 tgctgtattg cagatacggc atcttcggca acagggtacc gaccttgagc tggctctcgg
     9541 caaagcgcgc cttggcggtc aagcccagct tgctgtagtc gtcttcggcg cggttatcac
     9601 tgtcgcgtgg cagcaggtcg gtgccgctgc gatccggcga ggaatcgagc ttcacgccca
     9661 gcatacccag cgcatccagg ccaaaaccaa cggtgccctg ggtgtagccg gactcgatat
     9721 tcaggataaa gccctgcgcc cattcctcgc gcttgttctg cccggcaccg tcgtgcaagt
     9781 cacggttgag gtagaagttg cgcagttcca ggctggcttt gctgtcttcg ataaagcctg
     9841 cggcatgcgc cgccggcaac gacatgggca gcaggcacaa cgcgccaaga cgcaggccat
     9901 tttgcagagg ggtatttctt atcattgtgt gagtctctgt cggtgggttg cgggtaaatc
     9961 agtgctcggc cgcgctacgc cgcgggctgg ccacgtacac atcgccggcg aacaggcggc
    10021 gggcggtacg aatcaacgag gcgcgaatct tttccgggtt ctcggcgtca ccaggctcga
    10081 gggaaacggc aatctgcccg cccggatgct ccaggctgac cagggccgcg ccgctgatcg
    10141 gggccaggtc gtgggccacg ctgccgggca acgcgcaggc gctggccagg ccgatggcac
    10201 cggtggcggc cagggagcgg tggcagttgt gcggcatgaa atagcgggta ctgagggtgc
    10261 cgccctgccg ggctggcgcg agcaatacag gcttgggaat gaccatctgc gagacgtcac
    10321 ccaggcccat ggcgcgcccg gcctgcaggc ggatactttc cagacgctgc aacagttcgg
    10381 cgtcggaatc cagctcggcg ggtgtttcat agccggtctt gccgagagct tcggcatgca
    10441 tcagcaccat cggcatggcc atgtcgatac aggtcaccac aacgccgtca aactggtcgc
    10501 gcacctggcc ggtgggcagc agccggccgg tcttggctcc ggcggcatcg agaaaggtca
    10561 gcttgatcgg tgcagccgta ccaggtacgc catcgatgct ggtatcgccc tcatagcgca
    10621 ctttaccgtt gggggtgagc acctcggagt cgatcagggt attggtgttg acgttgcgaa
    10681 tgcgcacccg ggtaaccggt gcagcggcat tgaccaggcc atgctcgatg gcgaaaggcc
    10741 cgatggcgca gagcatattg ccgcagttgg gggccgtatc gacccggcgc tgcgccacca
    10801 tcacctggac gaacaggtaa tcgacatcgg cgtccggatg gctggaagcg ctgacaatcg
    10861 ccaccttact ggtctgcggg ctgccaccgc cgatgccatc gatttccagc tcatgccccg
    10921 agcccatgag tttgagcaac acctcgtcgc gcagagcagg attgctgggc aggtggttgg
    10981 ccagaaaaac cgggcctcgg gacgtaccgc cgcgcataag gacacaaggt attgcctgca
    11041 tgatgtctcc ggtgcaataa atatgtttat tgttgcgttg aataattatt gagcggcttc
    11101 gtttgatctg taaaatgcat atattcgtgc aattgatgct ttaaacgcat taatgcaagc
    11161 cgcaaaaacc cacgctaagg gccacgacag acgaatcgcg ggattggccg gttgcaatcg
    11221 atttgtgaac cgtaaaaatc acgccaatta ataggtattg cgcatgaatt acgaactaca
    11281 ggacctccgg gccttcgtga aaatcgccga attcggcaat tttcacgaag cggccaatgc
    11341 gatccatctg tcacagccgg cattgactcg gcgcatgcag aagcttgagg aagggctggg
    11401 tacccagttg cttgaccgaa ccacacggcg cgtcagcctg accgcagtgg gccgcgactt
    11461 cctgcccaag gccaggcgtt tactggacga tttcgagcgc tcgatcctcg gcatccgtga
    11521 actggccgag cgccagtccg gccaggtgac cctggcctgc atccccacgg cggcgttcta
    11581 cttcctgcca acggtgatcc gcgaattcaa cctgcagtat ccgaagatcc gcatccgcat
    11641 tctcgacctg agtgcccatg aagggttgga ggcagtacta cgcggcgagg ctgatttcgg
    11701 catcaatatg ctcagcggcc agagcgcgga gatcgacttc accccactgg tcgaggagcc
    11761 attcgtgctc gcttgccgcc gcgaccatcc tctggccgca caacctcagg tggcctggcg
    11821 cgaactcagt gattaccgcc tgatcggcgt cggtcgcctg agcggcaacc gcgtattgct
    11881 cgaccacggc ctggcccatc tggattggcg gcccagctgg ttctacgagg tgcaacatct
    11941 atcgacctcg ctggggatgg tcgaggccgg actgggagtg gcggccctgc ccagcctggc
    12001 gatgcctgcc gaagaccacc cgatactggt tcagcgcccc ctggtagacc ctgtcatcag
    12061 ccgtaccctg gggctggtgc gccgccacgg ttcatccttg tcgccagccg ccgaacaatt
    12121 cgtcgatacc ttgctgcgcc aatggccaag ctatcgctcg ttgggcaagg acaaacagta
    12181 aagatacaga gtacgggggg ccgcggtgca cgcgactaaa agaggaggcg ggaagacacc
    12241 cacgcctgtc gagtgcaagg taatctcggt agccaccggg aatcggctaa caggctgttg
    12301 aaaaactacc tgcgttggca atgctgcgtt aaaaacggcc tcggatgcga gcccagtcaa
    12361 aatgctcatt tacagcccgt aaactcgcgt gcgagcccag tccgtttttc ggccgttttt
    12421 gtggggccgc catcggtatt gctttgcctg cctcgcctac atttttcaac ggcctgctaa
    12481 ggaaacccca tgtcataccc ccgccttgtc agcatgacgg cgctcgcctt gatcgccttc
    12541 gccgccaact ccctgctgtg ccgggctgcg cttgccgacg gcagcatcga tgccgccagt
    12601 tttaccagca ttcgcctgat cgccggagcc gcagtgctct ggctgatcgt gcgcatccgc
    12661 gccggcagcg cggttggcaa aggcaactgg ctatcggcac tggccctgtt tgcctacgcc
    12721 gccgggtttt ccttcgccta tatcgaactg tctacggcca ccggcgccct gctgctgttt
    12781 ggcgcggtgc aaagcaccat gatcggttac ggcctgtacc gcggcgaacg cctgcgagtg
    12841 ccccagcttg gcggcttgtt gctggcgatt ggcgggctgg tcggcctact tctgccgggc
    12901 atctccgccc cgccgccact ggcctcggcg ctgatgctga gtgcgggcgt agcctggggt
    12961 atctactcac tgcgcggcaa aggtgccggc gatccgctgc aggtcaccgc cggtaacttc
    13021 cttcgggcgg tgcccattgc cctgattctc agcaccgcga cgctcggcga tatcaccctg
    13081 cagagcgctg ccggcattgc ctacgccatc gcttccggcg cgctggcctc cgggctgggt
    13141 tatgccgtct ggtacagcgc cctgccggcc ctgaaagcca ctacagccgc cagcgtgcaa
    13201 ctcagcgtac cggtgatagc cgccctgggt ggcgtggtcc tgctcgccga acccctcagc
    13261 ctgcgtctac tcctggccag cctggccata cttggcggca ttgcgctggt gatcctgacc
    13321 ggacaacgaa ccaaacccgc cagccgctaa caccgcaatc cagcggctac ggccacaaac
    13381 ctagcgaagt gcacgacctc gtgtagggtg cgccgtgcgc accaacggtc actgcctact
    13441 gagtagccat cagtggcgga caagcccccg acatggccgc cctaacagat tgcagaccct
    13501 acgtgctttg ccttcttggc ggtgcgcacg gcgcacccta catccgcagt cggctatcag
    13561 cgcttgatgg tcttgagcag atcttccggg gtgatatagc ccaccgcgcc gcccttgctc
    13621 tgggcgctgc caggttgcgg ctgatgcagg atatgaccct tcatcttgcc gatgatgtgc
    13681 atctcgcagg gtttgcagtc gaacttcagg gtcagtactt cgtcctgatg gatcagctgc
    13741 atatccgaga ctttggtgcg cacgccggta acgcccttgg cctgcttggg gcacaggttg
    13801 aaggagaagc gcaggcagtg cttggtgatc atcaccggca cttcgcccga ctcttcgtgg
    13861 gcctcgtagg cggcgtcgat cagttgcacg ccgtaacgct tgtagaaggt gcgggccttg
    13921 tcgttgtaga cgttggcgag gaaggtcagg tgcgattccg ggtacactgg cgctggcacg
    13981 ctgacagcct tgcgcccgcc acgcgggtga gcggcgacgc gggcctgggt cagcgcttcg
    14041 atggcttcgc ggcgcagggc cttgagttgc gagccgggga taaacggcac gacctcgcag
    14101 tcgatctgcg cgccggcgct gtaatagatg gtggtgccca gtttgctcaa gccatcggcc
    14161 agttgctcgc gagcctgctc ggcatccttg gcggcggcga acggcccggc caggctgacg
    14221 ctggcactga taccgtcatc gctggcgacg ctcacattca gttgctcgcc gcgcaggctg
    14281 agctgccagc tgaccgcaat gcgtcgctcg gacgaggtct tctgcagggc ctgctgccag
    14341 ttgtggtcga ggttgcgatg cagcggctgc agcggacgca cgcggtgcat ggcctcgggc
    14401 atctcgttgg gctcgacgcg gtaacggtag cgcttctcgc cgtcttcctc gaactcagct
    14461 ttaagctcgg cgatattggc gcggaaaccc accacttcac gcttgatcag cacgttaagg
    14521 ccatcgccgt tggacagcgg cgtgtcagtg acggcgatca ggtcgcgttt gttgaccttt
    14581 tccaccacac ccacggtcag cccggtaaag gtcggtgagt cgaacgcgcc aatgtccacc
    14641 ttgcggtcgg tgacgaaata gtcggtgctg ccgcggtgga aggtcttgtc ggtgtccggg
    14701 gtgaagaagt gctcggtacg cccactggag gcgcgttcca ggtgcggacg ctgctcgaga
    14761 atcgcatcca gctcgcggcg gtagtgcgcg gtgatgttct tcacgtagct catgtctttg
    14821 tagcgccctt cgatcttgaa cgagcgcacg ccggcatcca ccaggtagat caggttgtcg
    14881 gtctggttgt tgtccttcat cgacagcagg tgcttatcaa acgccaccac gcggccctga
    14941 tcgtccttga gggtgtacgg caggcggcag gcctgggagc agtcgccacg gttggcgctg
    15001 cgcccggtct gcgcgtggga gatattgcac tgcccggaga acgccacgca cagcgcgccg
    15061 tggataaaga actcgaccgc cgaatcgaca ttgcggcaga tattgccgat ctcttccagg
    15121 ttcagctcgc gggccagcac cagctgggag aagccagcgc catcgaggaa gcgggctttt
    15181 tccagggtgc ggatatcgca ctgggtgctg gcgtggatct cgatgggcgg aatgtccatc
    15241 tccatcacgc ccatgtcctg cacgatcagc gcatcgacgc cggcctcgta cagctgccag
    15301 atcatctgtc gcgccggttc cagctcatcc tcatgcagga tggtgttgag ggtgacaaac
    15361 acccgcgcat ggaacaggtg ggcaaactgc accagctcag caatatccgc cacgctgttg
    15421 ctggcgttgt gccgcgcacc gaagccgggg ccgccgatat acacggcatc ggcgccgtgc
    15481 aggatggctt ccttggcaat cgcggtatcg cgcgccgggc tcaggagttc gaggtggtgc
    15541 ttgggcaggg acatggtttc gctatcgggc agtcaatcag gccgagcatt ttactcggaa
    15601 tagctgattt gtctgtgccc gcttggcagc cgcacaacaa ctgtataggc atacagataa
    15661 aagcct
//
LOCUS       DASRUM010000031         8704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2357745, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000031 DASRUM010000000
VERSION     DASRUM010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8704
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2357745"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..670
                     /gene="ybgF"
                     /locus_tag="VFF22_02465"
     CDS             29..670
                     /gene="ybgF"
                     /locus_tag="VFF22_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HZX15718.1"
                     /translation="MLFNQLQQMQEEIAQLRGTLEEQQNEIQRLKQESLERYQDLDRR
                     VSGGAAGAAANQNSSAAGAVNAIGTPTPPAAGQAQAPASNEPADPAKEKLFYEAAFDL
                     IKAKDFDKASQAFAAFLRKYPNSQYAGNAQYWLGEVNLAKGDLQGAGQAFARVSQTYP
                     SHAKVPDSLFKLADVEQRLGNSDKAKGILQQVIAQYPGSSAAQLAQRDLQRLP"
     gene            788..1435
                     /gene="queE"
                     /locus_tag="VFF22_02470"
     CDS             788..1435
                     /gene="queE"
                     /locus_tag="VFF22_02470"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HZX15719.1"
                     /translation="MQDTLRITEIFYSLQGETRTAGLPTVFVRLTGCPLRCQYCDTAY
                     AFSGGEIQSLDAIIEQVAGYKPRYICVTGGEPLAQPNCIPLLKRLCDAGYEVSLETSG
                     ALDVSAVDPRVSKVLDLKTPGSAEVARNRYENIELLTLNDQVKFVICSREDYDWAVSK
                     LIQYRLDQRAGEVLMSPSHHELDARSLAEWIIADNLPVRLQMQLHKILWNDEPGH"
     gene            1437..2111
                     /gene="queC"
                     /locus_tag="VFF22_02475"
     CDS             1437..2111
                     /gene="queC"
                     /locus_tag="VFF22_02475"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HZX15720.1"
                     /translation="MSDKKAVILLSGGLDSATVVALAKADGYSCYTMSFDYGQRHRSE
                     LQAAEQVARQLGVVEHKVIGLNLNGMGGSALTDASIDVPESPTEGIPVTYVPARNTVF
                     LSLALGWAEVLGARDIFIGVNAVDYSGYPDCRPEFIEAFERMANLATKAGVEGQGFRI
                     QAPLQAMSKAEIVQAGVQQGVDYALTVSCYQADDQGRACGKCDSCRLRAAGFATAGLV
                     DPTRYF"
     gene            2169..2244
                     /locus_tag="VFF22_02480"
     tRNA            2169..2244
                     /locus_tag="VFF22_02480"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2202..2204,aa:Lys,seq:ttt)
     gene            2415..3473
                     /gene="nadA"
                     /locus_tag="VFF22_02485"
     CDS             2415..3473
                     /gene="nadA"
                     /locus_tag="VFF22_02485"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HZX15721.1"
                     /translation="MTQISERLLVQAHLDAKQPKPLTPEQEAFYRTEIAAELKKQNAV
                     LVAHYYCDPVLQALAEETGGCVSDSLEMARFGNQHPAQTVLVAGVKFMGETAKILNPE
                     KRVLMPTLEATCSLDLGCPVEEFSAFCDQHPQRTVVVYANTSAAVKARADWVVTSSCA
                     VEIVEHLMDNGESIIWAPDQHLGRYIQNKTGADMLLWDGACIVHEEFKAKQLEDMKAL
                     YPDAAVLVHPESPTAVIDLADAVGSTSQLIAAAQRLPNKTFIVATDAGIFYKMQQLCP
                     DKEFVAAPTAGNGATCRSCAHCPWMAMNTLQRTLECLREGSNEIFVDPALIPKAIKPL
                     KRMLDFTQAARLKLSGNA"
     gene            complement(3530..4963)
                     /locus_tag="VFF22_02490"
     CDS             complement(3530..4963)
                     /locus_tag="VFF22_02490"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZX15722.1"
                     /translation="MSLLRPTLLTLACLLAAPAMASDLPSLGDASSAIVSPQQEHQLG
                     RAWLSLVRGQVSQLDDPQLKDFVETSVYRLSETSQLQDRRLEFVLLNSPQINAFAAPG
                     GIVGVNGGLFLYAQTEAEYASVMAHELAHLSQRHFARGLEAQQRMQIPVMAGLLAGII
                     AAAAGAGDLGIAAIASTQAAAMQEQRRFSRQNEQEADRIGLVNLEKAGFDPRAMPSMF
                     ERLMRQYRYDRKPPEFLLTHPVSESRIADTRNRAEQYTAKGVTDSVRYQLMRARVQLT
                     YEETPGMAAKRFRNMLDENPQMEAARYGLAIAQIKSGQHKQARESLQPLLESSPNDVA
                     YNLAQIELDMAANRLSDAQSRTSRLMTLYPNNYPVNQAHIDLLMKQGRIQEAERALDD
                     MLKSRGKDPDVWYQVAEVRGLSGNTIGLHQARGEFFALVGDYNQALEQLDFAKRRANN
                     NFQLASRIDARQRELIEEQRIIEQMLR"
     gene            5070..5321
                     /locus_tag="VFF22_02495"
     CDS             5070..5321
                     /locus_tag="VFF22_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003172483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HZX15723.1"
                     /translation="MTDMQWRAEDFDAQLDACGLNCPLPLLKAKLELNRLASGAVLKV
                     EATDAGSQRDFRAFASLAGHSLLREEVDNGVYRYWLRKA"
     gene            5389..6453
                     /locus_tag="VFF22_02500"
     CDS             5389..6453
                     /locus_tag="VFF22_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZX15724.1"
                     /translation="MFKVFQDWLHRYFSDEQAVVLAVLLVLGFAAILNLGGMLAPVLT
                     GLVLAFLMQGLVNAFERMRLPQMVAVWLVFTLFISMLGLFLLVLMPLLWQQLSTLFNE
                     LPRMATEWQSVFLLLPERYPNLITDEQIVQLIDSMRGEAGKFGQWALSFSLSSLPMLL
                     TIMIYLVLVPILVFFFLKDRELISAWFRGYLPRERALITQVAEEMNKQIANYIRGKVI
                     EIIICGVVTYIAFAYLGLNYAALLALLVGLSVVVPYIGAVVVTVPVALIGLFQWGFGD
                     QFIYLMVVYGIIQALDGNVLVPLLFSEAVNLHPVAIICAVLLFGGMWGFWGVFFAIPL
                     ATLFKAVIDAWPRSEQIAAG"
     gene            complement(6506..6979)
                     /locus_tag="VFF22_02505"
     CDS             complement(6506..6979)
                     /locus_tag="VFF22_02505"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZX15725.1"
                     /translation="MAVALDTPVADFQAEATNGQTVQLSALKGQQVVIYFYPKDSTPG
                     CTTEGQGFRDQFPAFQAANTLIFGVSRDSLKSHENFKCKQEFPFELISDKDEAVCQLF
                     DVIKLKKLYGKEYLGVDRSTFLIDKAGVLRQEWRGVKVPGHVDAVLAAAQALNKA"
     gene            complement(6993..7550)
                     /locus_tag="VFF22_02510"
     CDS             complement(6993..7550)
                     /locus_tag="VFF22_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein R"
                     /protein_id="HZX15726.1"
                     /translation="MSTPLVREQFLLISALGPNAMELTNVLCRTSHENRCAVVSTRLS
                     RHGEYSALVLQISGSWDALARLESSLPALAKKHSFSVNVIRSAASESRPQALPYVAYV
                     SSAYRPDILNELCQFFLDHHVELENLTCDTYQAPQTGGTMLNATLTVTLPAGTQISWL
                     RDQFLDFADALNLDALIEPWRPQNP"
     gene            7786..8664
                     /gene="dapA"
                     /locus_tag="VFF22_02515"
     CDS             7786..8664
                     /gene="dapA"
                     /locus_tag="VFF22_02515"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003317384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZX15727.1"
                     /translation="MIAGSMVALVTPMDAQGGLDWDSLSKLVDFHLQEGTNAIVAVGT
                     TGESATLEVPEHIEVIKRIVDQVAGRIPVIAGTGGNSTRESVELTRAAKDVGADACLL
                     VTPYYNKPTQEGLYLHFRHIAESVAIPQILYNVPGRTVCDMLPETVERLAKIDNIIGI
                     KEATGDLQRGQEVLDRVSKDFLVYSGDDATAVELMLMGGKGNISVTANVAPRAMSDLC
                     AAAMRGEAAIARAINDRLMPLHKALFIESNPIPVKWALHEMGLMPDGIRLPLTWLSPR
                     CHEPLRQALRQSGVLV"
BASE COUNT         1641 a         2412 c         2747 g         1904 t
ORIGIN      
        1 ggctgcggcc cctgtcacgg cgcagggtat gctgttcaac cagttgcagc agatgcagga
       61 agagatcgcg cagctgcgcg gtacgctgga agagcaacag aatgaaatcc aacgtctgaa
      121 acaagaaagc ctggagcgtt atcaggatct tgatcggcgt gtgagtggtg gtgcggcagg
      181 tgctgcggct aaccagaatt cttcagccgc tggcgcagtc aatgccatcg gtacgccaac
      241 cccgccagct gcggggcagg cacaagcgcc agccagcaat gagccggccg atccggcaaa
      301 ggaaaagctg ttctacgaag cagcctttga cctgatcaaa gccaaggact tcgataaggc
      361 cagtcaggcc tttgcagcgt tcttgcgcaa atacccgaat agtcagtacg ccggcaacgc
      421 gcagtactgg ctcggagagg tgaacctggc caaaggtgat ctgcagggcg caggccaggc
      481 atttgcacgg gtcagccaga cttacccgag ccacgccaaa gtgccggact ccttattcaa
      541 gctggctgac gtcgagcagc gccttggcaa cagcgacaag gctaaaggta ttctgcagca
      601 ggtaattgcc cagtatccgg gcagttcggc ggctcagctg gcgcagcgtg atttgcagcg
      661 tttgccgtaa aagctgaact tcgaatcaag aaacccgcgc tttgcgcggg ttttttcgtt
      721 agaatccgcg ccccttttcc cgcaacggag gcggacggcc tgtttagctg tcacgcccgt
      781 ggctgatatg caagataccc tgcgcattac cgagattttc tactcgctgc agggcgagac
      841 gcgcaccgcc ggcttgccga ctgtgttcgt tcgcctgacc ggctgtcctt tgcgttgcca
      901 gtactgcgac accgcctacg ccttcagtgg cggcgagatc cagtcgctgg acgccatcat
      961 cgaacaggtt gccggctata agccgcgtta tatctgcgtg accggtggtg agccgttggc
     1021 gcaacccaat tgcattcctc tgctcaagcg cttgtgcgat gccggctatg aagtgtcgct
     1081 ggaaaccagt ggcgcgctgg atgtatcggc ggtcgatccg cgagtgagca aggtgctcga
     1141 cctgaaaacc ccaggctcgg ccgaagtagc acgtaaccgc tacgagaata tcgagctgct
     1201 gacgctaaat gatcaggtca agttcgttat ttgctcgcgt gaggattacg actgggcggt
     1261 gagcaaattg atccagtacc gcttggatca gcgcgccggt gaggtgctga tgtcgcccag
     1321 ccaccatgag ctggatgcgc gctcgctggc cgagtggatc attgccgaca acctgcccgt
     1381 gcgcctacag atgcagctgc acaagattct ctggaatgat gagccgggac attgatatga
     1441 gcgataagaa agcggtaatc ctgctgtcag gcggcctgga ctcggccacc gtggtcgcgt
     1501 tggccaaagc cgatggctac agctgttaca caatgagttt cgattacggc cagcgtcacc
     1561 gctctgaatt gcaggcggct gagcaggtgg cgcggcagct gggtgtggtc gagcacaagg
     1621 tgatcggcct gaatctcaat ggcatgggcg gctcggcgtt gaccgacgcc agtatcgatg
     1681 tgccggaaag cccgaccgaa ggtattccgg tgacctacgt gccagcacgc aatacggtgt
     1741 ttctctccct ggcgctgggt tgggctgagg tgctgggtgc gagggatatt tttatcgggg
     1801 tgaacgcggt ggattattcc ggttacccgg attgccgccc ggagtttatt gaggccttcg
     1861 agcgtatggc caatctggcc accaaggccg gtgtagaagg gcagggcttt cgtattcagg
     1921 ctccattgca ggccatgagc aaggccgaga tcgtccaggc aggcgttcag cagggcgttg
     1981 attacgcact gactgtttcc tgctaccagg ctgatgatca aggccgcgcc tgcggaaaat
     2041 gcgacagctg ccgtctgcgt gctgcgggtt ttgctaccgc cgggctggta gatcccactc
     2101 gttatttcta aaaagttttc gcgaagtgtt gaattcctga attaaatcag tatcatgcgc
     2161 ctcgcgtcgg gtcgttagct cagttggtag agcagttggc ttttaaccaa ttggtcgtag
     2221 gttcgaatcc cacacgaccc accatatttc cttcctcgga aggtcaagaa gcctgaaagt
     2281 tctgattaaa ggaattttcg ggcttttttg tatctgtcac ttttaccacc ggcgcgtggt
     2341 tgcaacctgc ctgctccagc gctattcagg cgggtatact cgctgcactg aaagcatatt
     2401 cagagcctga tgacatgacc cagatttccg aacgcctgct cgtgcaagcc caccttgatg
     2461 ccaagcagcc caagccgctg acgcccgagc aagaggcttt ctaccgtact gaaattgctg
     2521 ccgagctgaa aaagcagaac gccgtgctgg tcgcgcatta ttactgtgac ccagtgctgc
     2581 aggcgctggc cgaggaaacc ggcggctgcg tttccgactc gctggaaatg gcccgtttcg
     2641 gcaatcagca cccggcgcag accgtgctgg tggccggcgt taaatttatg ggcgaaacgg
     2701 cgaaaatcct caatccggaa aagcgcgtgc tgatgccgac gcttgaagcg acctgctcgc
     2761 ttgatctggg ttgcccggtg gaagagttct cggcgttctg cgatcagcat ccacagcgca
     2821 cggtggtggt gtatgccaac acctctgcag cggtgaaggc gcgcgccgat tgggtggtga
     2881 cttccagttg tgcggtggag atcgtcgagc acctgatgga caacggcgaa agcatcatct
     2941 gggcgccgga ccagcatctg ggccgttata tccagaacaa gaccggcgct gacatgctgc
     3001 tgtgggacgg tgcctgcatc gtccacgagg agttcaaggc caagcaactg gaagacatga
     3061 aggcgctgta cccagacgca gccgtgttgg ttcaccctga gtcgccgacc gcagtgatcg
     3121 atctggccga tgcagtgggc tctaccagcc agctgatcgc agctgcccag cgtctgccga
     3181 acaagacctt tatcgtcgcc accgacgccg gcatcttcta caagatgcag caactgtgtc
     3241 cggacaagga atttgtcgcc gctccgactg ccggtaacgg cgctacctgt cgcagctgcg
     3301 cgcattgccc gtggatggcg atgaataccc tgcagcgcac cctcgagtgc ctgcgcgagg
     3361 gcagcaacga gatattcgtc gaccctgcat tgatccccaa agcgatcaaa ccgctcaagc
     3421 gcatgctcga cttcactcag gctgcgcggc tcaagctgag cggaaacgcc taagcgaacc
     3481 gcataaaaag ccaggcattt gcctggcttt ttcttttctg gctacctgct caacgcaaca
     3541 tttgctcgat gatgcgctgc tcctcgatta gctcgcgctg tctggcgtcg atgcgcgagg
     3601 ctagttggaa gttgttgttg gcgcggcgct ttgcgaagtc gagttgttcc agagcctggt
     3661 tgtagtcacc gaccagggca aagaactcgc cgcgtgcttg atgcaggccg atggtgttgc
     3721 cgctcaggcc gcgtacttct gctacttggt accaaacgtc cgggtccttg ccgcggcttt
     3781 tcagcatgtc atcgagggcg cgttcggctt cctgaatgcg gccctgcttc atcagcaggt
     3841 cgatatgtgc ctggttgacc gggtagttgt ttgggtagag ggtcatcagt cggctagtcc
     3901 ggctttgcgc gtcgctcagg cgattggccg ccatatccag ttcaatctgg gccaggttgt
     3961 aggcgacatc gttcggggag ctttccagca gcggttgcag ggactcgcgc gcctgcttgt
     4021 gctggccgct tttgatctgt gcaatcgcca gcccgtagcg agcggcttcc atctgtgggt
     4081 tttcgtcgag catattgcga aaacgcttgg cggccatccc cggggtttct tcgtaggtca
     4141 gctgcacgcg ggcgcgcatc agctggtagc gcacgctgtc ggtgacgcct ttggcggtgt
     4201 actgctcggc acggttgcgg gtgtcggcga tacgcgactc cgacactggg tgagtcagga
     4261 ggaattccgg cggcttgcgg tcgtagcggt actggcgcat caggcgctcg aacatgctgg
     4321 gcatggcgcg tgggtcgaag ccggcttttt ccagattgac caggccgatg cgatcagctt
     4381 cctgttcgtt ctgccgagag aagcggcgct gctcctgcat ggctgcggcc tgggtcgagg
     4441 cgatggctgc aatacccaga tccccggcac cggcggctgc ggcgatgatg ccggcgagca
     4501 ggccggccat caccgggatc tgcatgcgct gctgcgcttc tagccctcga gcgaagtggc
     4561 gttgtgacaa gtgcgccagt tcgtgagcca tgactgaggc gtactcggct tcggtctgcg
     4621 catacagaaa caggccgccg ttgaccccga caatcccgcc tggggcggca aaggcattga
     4681 tctgcgggct gttgagcagg acgaattcca ggcggcggtc ctgcaactgg ctggtttcgc
     4741 tcaggcggta cacgctggtt tcgacgaagt ccttgagctg tggatcgtcg agttggctga
     4801 cttggccgcg taccagactc agccaggcgc ggcccagctg gtgttcttgc tgcggggaaa
     4861 cgatggcgga actggcatcg ccaagggagg gcaggtcact ggccatggcg ggggctgcca
     4921 gcaggcaggc cagcgtcaac agggtagggc gcagaaggct catgcacaaa gctcgtgtcg
     4981 aacaaaggcg ctactgtagc tggctggcgg ctgtgctggc cagggttgtg cggccttggg
     5041 tatccttgcg gccttcctgg agtagctcaa tgactgatat gcaatggcgt gccgaagact
     5101 tcgatgcgca actggacgct tgcgggctca actgcccgct acccttgctt aaggccaagc
     5161 tggaactcaa tcgcctggcc agcggtgctg tgctcaaggt tgaggccacc gatgccggtt
     5221 cgcaacggga tttccgtgcc ttcgcgagcc tggccgggca tagtctgttg cgtgaagagg
     5281 tcgataatgg cgtttaccgc tattggttgc gcaaggccta gggtctgctg cgctaagcgc
     5341 tgcttgcgag gcgttataga cactgtgttt cccaggtaag gagtcgcaat gttcaaggtg
     5401 tttcaggatt ggctgcatcg ctatttttcc gatgagcagg cggtggtgct tgcggtgttg
     5461 ctggtgcttg gctttgccgc gatcctgaac ctgggcggca tgctagctcc ggtactgacg
     5521 gggctggtgc tggcgtttct gatgcagggc ctggtgaatg cctttgagcg tatgcgcttg
     5581 ccgcagatgg tcgcggtgtg gctggtgttc accctgttta taagcatgct tgggctgttt
     5641 ctgctggtgt tgatgccact gctctggcag cagctgagta ccttgttcaa tgagctgccg
     5701 cgcatggcta cggagtggca atcggtgttc ctgctgctgc ccgagcgcta cccgaatctg
     5761 attacggatg agcagatcgt gcagctgatt gacagcatgc gtggcgaagc cggcaagttc
     5821 ggccagtggg cgctgtcgtt ctccctgtcc agtctgccga tgctgctgac catcatgatc
     5881 tacctggtgt tggtgccgat tctggtgttc ttcttcctca aggatcggga gctgatcagt
     5941 gcatggtttc gcggctatct gccgcgtgag cgcgcgttga tcactcaggt ggccgaggaa
     6001 atgaacaagc agatcgccaa ctacatccgc ggcaaggtga tcgaaatcat catctgcggc
     6061 gtggtcacct atatcgcgtt tgcctacctg gggctcaact acgcggcgct gctggccttg
     6121 ctggtgggct tgtcggtggt ggtgccgtat atcggcgcgg tagtcgtaac ggtgccggtg
     6181 gcgctgatcg gcttgttcca gtggggcttt ggtgaccagt tcatctacct gatggtggtc
     6241 tacgggatca tccaagcgct ggacggcaac gtattggtgc cgctgctgtt ctccgaggcg
     6301 gtcaacctac acccggttgc gatcatctgc gcggtgctac tgtttggcgg catgtggggc
     6361 ttctggggcg tgttcttcgc catcccgctg gccacgctgt tcaaggcagt gatcgacgcc
     6421 tggccgcgca gtgagcagat agcggccggt taatctgcag gcaataaaaa gccggtcaac
     6481 tgaccggctt ttttacgcgc gcgattcagg ctttattcag tgcctgggcc gcagccagta
     6541 ccgcatcgac atgccctggc actttcacgc cgcgccattc ctggcgcagt acgccggctt
     6601 tgtcgatcag gaaggtgctg cggtcgacgc ccaggtattc cttgccgtag agcttcttca
     6661 gcttgatcac atcgaacagc tggcaaacgg cttcatcctt gtcggaaatc agctcgaagg
     6721 ggaattcctg tttgcacttg aagttctcgt gggatttcag gctgtcgcgc gatacgccga
     6781 agatcagcgt gttggctgct tgaaacgctg ggaactgatc acgaaaaccc tggccctcgg
     6841 tggtgcagcc gggggtgctg tccttcgggt agaagtagat caccacttgc tggcccttga
     6901 gtgcggacag ctgaacggtc tggccgttgg tggcctctgc ctgaaagtct gcaaccggag
     6961 tgtcgagtgc tacggccata aaatgctgct ccttatgggt tctgtggacg ccaaggttca
     7021 atcaacgcat ccaaattcaa cgcatcggca aagtcgagaa attgatcgcg caaccagctg
     7081 atctgcgtac cagccggcag ggttacggtc agcgtggcgt tgagcatggt gccgccggtc
     7141 tgcggggcct ggtaggtatc acaggtgagg ttttccagtt cgacgtgatg gtcgagaaag
     7201 aactggcaca gttcattgag aatgtccggg cggtaggccg agctgacata cgccacataa
     7261 ggcagcgcct gtgggcggct ttccgaggcg gcgctgcgaa tcacgttgac cgagaagctg
     7321 tgcttcttcg ccagcgccgg caggctcgat tccagacggg ccagggcatc ccagctgccg
     7381 gatatctgca agaccagtgc gctgtactcg ccatgccgac tgaggcgggt gctgaccacg
     7441 gcgcaacggt tctcgtggct ggtgcggcag agcacgttgg tcagctccat ggcgttgggg
     7501 ccaagggcgc tgataagcag gaattgttcg cgaactaggg gggtggacat gcagccttcc
     7561 taaacgataa gcggtcggcg ctgtgcatac agcgtcgatc aaagccggaa gggtagcgaa
     7621 aagcaccgct caggggaatg ccggcggggc tgggcggtga ggagtgtgcc gcgtttggcc
     7681 gagaaagatt gtatacgcgt tgcatcacag cctcggtact tcgcttgtac aaggcaggtg
     7741 gccccagtac cattacggct ctctttttcc ggcaggagcg gttgcatgat tgcgggcagt
     7801 atggtggcac tggttacgcc catggatgca caaggtggtc ttgattggga cagcctgagc
     7861 aaactggtgg atttccacct gcaggagggc accaatgcca tcgtcgcagt cggcactacc
     7921 ggcgaatcgg cgacccttga ggtgcctgaa cacatcgaag tgatcaagcg catcgtcgat
     7981 caggttgccg ggcgcattcc ggtcatcgcc ggcaccggtg gcaactccac ccgtgaatcg
     8041 gtcgaactga cccgtgccgc caaagatgtc ggtgccgatg cctgcctgct ggtcaccccg
     8101 tattacaaca agccgactca ggaaggtttg tatctgcact tccgccacat cgccgaatcg
     8161 gttgccatcc cgcagatcct ctacaacgtg ccaggccgta cggtctgcga catgctgccg
     8221 gagacggttg agcgtttggc caagatcgac aacatcatcg gtatcaagga agccaccggc
     8281 gacctgcagc gcggtcagga agtactggat cgcgtgagca aggacttcct ggtctattcc
     8341 ggtgatgacg ccaccgccgt tgagctgatg ctgatgggcg gcaagggcaa catttcggta
     8401 accgctaacg tcgccccgcg cgcgatgagc gatctgtgtg cggcggccat gcgtggtgaa
     8461 gcagcgattg cccgtgcgat caacgaccgc ttgatgccgc tgcacaaggc gctgtttatc
     8521 gaatccaacc cgattccggt gaaatgggct ctgcatgaaa tgggtttgat gccagacggt
     8581 atccgtctgc cactgacctg gctcagccca cgttgtcatg aaccgctgcg tcaggccctg
     8641 cgccagtccg gcgtattggt ttaattgagg aattactacg catgaagcga ctcaccggac
     8701 tctc
//
LOCUS       DASRUM010000032        19785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1354940, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000032 DASRUM010000000
VERSION     DASRUM010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 19785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19785
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354940"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /gene="lysS"
                     /locus_tag="VFF22_02520"
     CDS             complement(<1..1387)
                     /gene="lysS"
                     /locus_tag="VFF22_02520"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011532564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZX15728.1"
                     /translation="MSDQQLDQQALSHEDQQQEENKLVAQRKEKLAAIREQGNAFPND
                     FRRDNYCADLQKQYAEKTKEELAEAAIPVKVAGRIMLNRGSFMVIQDMTGRIQVYVNR
                     KTLSEETLAAVKTWDMGDIISAVGTLARSGKGDLYVEMTDVRLLTKSLRPLPDKHHGL
                     SDTEQRYRQRYVDLIVNEDVRQTFRVRSQVIAHIRSFLMQRDFLEVETPMLQTIPGGA
                     AAKPFETHHNALDMQMFLRIAPELYLKRLVVGGFEKVFEINRNFRNEGVSTRHNPEFT
                     MLEFYQAYADYEDNMDLTEELFRELAQLVLGSTDVPYGDKVFHFGEPFVRLSVFDSIL
                     KYNPELTADDLTDLDKARAIAKKAGAKVLGFEGLGKLQVMIFEELVEHKLEQPHFITR
                     YPFEVSPLARRSDDDPSVTDRFELFIGGREIANAYSELNDAEDQAARFMQQVADKDAG
                     DDEAMHYDADFV"
     gene            complement(1475..2570)
                     /gene="prfB"
                     /locus_tag="VFF22_02525"
     CDS             complement(join(1475..2497,2499..2570))
                     /gene="prfB"
                     /locus_tag="VFF22_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933612.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HZX15729.1"
                     /translation="MEINPILNSIKDLSERTLTIRGYLDYDHKHDRLVEVNRELEDPN
                     VWNNPEYAQNLGRERASLALIVDTLDDLHGSLADSRDLLDMAVEEDDEGAVNDVAAEI
                     ERLREILEKLEFRRMFSGEMDPNNAYLDIQAGSGGTEAQDWANMLLRMYLRWADKRGF
                     DATIMELSAGEVAGIKGATLHIKGEYAFGWLRTEIGVHRLVRKSPFDSGNRRHTSFTA
                     VFVSPEIDDNIEIEINPADLRIDTYRSSGAGGQHVNTTDSAVRITHVPTNTVVSCQNE
                     RSQHANKDTAMKMLRARLYEQEVQKRNAASQVLEDSKSDIGWGHQIRSYVLDASRIKD
                     LRTNIERSDCDKVLDGDIDEYLVASLKQGL"
     gene            2740..4383
                     /locus_tag="VFF22_02530"
     CDS             2740..4383
                     /locus_tag="VFF22_02530"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZX15730.1"
                     /translation="MSSSHSLRSRFAILIALLVASLSWLLGTLIGHDASQRIREEVGR
                     DLAEVSFMMVDRLDRDMQSRAAMLQVIGKLRALNQPDDIVEVRVLLDRLQIEFPSIAW
                     IGFTDAKGTVLASSDGVLEGASIAQRPVFLEAQKNVFIGDVHEAVLLAKLLPNPTGEA
                     MKFVDISLPVFAPDGSLVGVLASHLSWSWADEVRLAILEPIQKRRNVEFFVIGEDRTV
                     LLAPKELIGQRLHLPALNDVAQRKDLWAVQEWPDGKQYLTGMALSQGHMDYKGLGWTV
                     IARQNLDEAYAPARETTRYILLWGSGLALLFALLGWLLAGYFTRPLRQIAEAADRLSA
                     GEISVIPELKSTREIEILSNSIRHLVESLTHQQNALGAMENLAHHDALTGLPNRVALE
                     SYLPRAQQRSQSNNNCLALFYLDLDGFKPINDQFGHAAGDQLLREVAVRLRGCLREGD
                     LVARLGGDEFLMVLQVPSSEALGQAQLVAERTLAALSAPVDLGNNQVKVGCSIGGALW
                     PLDSSNLAEALELADQALYRAKHAGKNCVIFHSQADADG"
     gene            complement(4412..6016)
                     /locus_tag="VFF22_02535"
     CDS             complement(4412..6016)
                     /locus_tag="VFF22_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZX15731.1"
                     /translation="MQQLEEEYDYLIVGAGPAGCLLANRLSADPAIRVLLLEAGGRDN
                     YPWIHIPVGYLYCIGNPRTDWCYDTAPEPGLNGRSLKYPRGRVLGGCSSINGMIYMRG
                     QARDYDGWAAAGNPGWAWQDVLPLFKRTENHYGGGDALHGGTGEWRVEQQRLTWDLLE
                     AFRQGAAQSGIASIDDFNTGDNEGCSYFQVNQRRGVRWNASKAFLRDIRKRPNLRVLT
                     DADVQRVVLGNGRAEALNLHWQGQDRTIKAQREIILCAGAIGTPALLQRSGIGPRSLL
                     QRLGIGVQHELAGVGENLQDHLQLRLIYKVSEVPTLNQIAGSLWGKMGMGVEYLLSRS
                     GPLSMAPSQLGAFARSCAEQPSANLQYHVQPLSLERFGEPLHAFPAFTASVCNLRPYS
                     RGKVEIRSLNSAETPLIQPNYLSDTRDLQVAADAIRLTRRIVAAPALAPFRPEEYKPG
                     PSCQSEEDLQRAAGEIGTTIFHPAGTCKMGAGADAVVDAQLRVHGVPGLRIADASIMP
                     NIVSGNTCSPVLMIAEKAAQLILAST"
     gene            complement(6123..7508)
                     /gene="xrtQ"
                     /locus_tag="VFF22_02540"
     CDS             complement(6123..7508)
                     /gene="xrtQ"
                     /locus_tag="VFF22_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012182.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase Q"
                     /protein_id="HZX15732.1"
                     /translation="MMAWLRTGRLRAAAHPPGWLWLLLPTLALWPVWRWSAQRMSDGS
                     DDPFGIVALAALLLMIWRERRRLSAEARLPWLLLALLLCAATALAPGLPPLLRGVLAV
                     LTLFAVVLALRASGQALLAWLGLGLLALPIMSSLQFFIGYPLRVLTAEVSAWLLRSGG
                     LEVLRQGSTLEVAGQLVMVDAPCSGIQMAWVAYFTAFTTAAWLRLSDRQLLRRLPVLG
                     VLVLAGNILRNSLLVLQETGRLDWPGWMHEGTGLLVFVGVCTLVLRYVAAGRTRAQMV
                     AEPVCCRPLKPIDPVMQRLLVLSFALLALWPMLAKPIASEPRHGNFVEWPQQYAGDAL
                     RPLALSAVEQRFAEQFPGAIARFSAGARVVTLRHVMRPTRKLHPAADCYRGLGYRIQA
                     MVLEQRTDAAGLQRCFIASGHGAKLRVCEYIEDANGKRFSDNSAWYWSALAGNSSGPW
                     LAVSTAELSPP"
     gene            complement(7509..9533)
                     /locus_tag="VFF22_02545"
     CDS             complement(7509..9533)
                     /locus_tag="VFF22_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017518861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HZX15733.1"
                     /translation="MFHSLFTSRTRVGRRLALLFALLLSQPALTCAQSAVERSESPYF
                     VVQSDDPSVDRLPLKSTQVEVRVLGVIVDVRVVQQYRNEGTRALEARYVFPGSSRAAV
                     YGMTVRLGDRELQAQIREKQKAQQEYQQAKSMGKTAALLEQHRENVFQMNVANILPGD
                     VVDVELRYTELLLPTDGVYSFVFPTVVGPRYNGAGGSESGKGGPWVSTPNLPEGQTTR
                     DSFALKVELQSPVPLVDIRSPSHRIELQQIMQSQALVSLGAEEGQSNNRDFVLDYRLS
                     GASFESGVLLSKGVDENFFLALIAPPAKVKSQMLVPREYIFVVDISGSMHGFPLDTAK
                     HLLKNLVGALRPSDSFNVLLFSGSSSMLAAESQTATPANIERALDMLDTTMGSGGTEL
                     LPALRQALAVPADPERARSFVVVTDGYVTVEREAFELVRNNLDKANLFAFGIGKSVNR
                     QLIEGLARAGQGEPFVILNKASADEQAERFRQMIDAPVLANPQVRFDGLEVYDVEPVA
                     LPDLFAQRPLVVFGKWRGEARGNLLVEGHGAAGPYRVEVPIESQMASEGNRALGYLWA
                     RHKVASLSDQETLEGGYTYSQAILDLGLKYSLLTAYTSFIAVDEQVRNPDPAAADSVK
                     QTLPLPQEVSNQAIGAVVSSTPEPSTWLMLLVAALGMGWLARQHARQEQN"
     gene            complement(9658..10407)
                     /locus_tag="VFF22_02550"
     CDS             complement(9658..10407)
                     /locus_tag="VFF22_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HZX15734.1"
                     /translation="MITRGLLDQLLKSGQDLMQQKSAGSSAGGSAGGLGGALGGLLGG
                     AGKGGQGGDLGTLLKGAGGGALAAGALGMLLGNKSARKMGGKALTYGGLAALGVVAYK
                     AYSNWQAQQANAPQTEPQTLDRLPAPQAELHSQAILKALVGAAKADGHVDARERQLIE
                     GELVKLTSDGELQHWLQAELNKPLDPAEIARAASTPEMAAEMYIASVLMVDEEHFMER
                     AYLEELARQLKLDPALKSELEAQVRLQLDVS"
     gene            10697..11368
                     /locus_tag="VFF22_02555"
     CDS             10697..11368
                     /locus_tag="VFF22_02555"
                     /inference="COORDINATES: protein motif:HMM:NF017815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HZX15735.1"
                     /translation="MPQRANRPAHRLLRSLLASCWLFSPVLVADAPSTQHSALATALS
                     DTRLASWLNLLDNSAGDNERAQLERVNHFINRSVSFVSDQQAWGAEDYWATPAQTLSL
                     GKGDCEDFAIAKYFSLVRIGVPSEKLRLTFVKALKQNQAHMVLAYYPSASEQPLILDN
                     LDPRIRPASERGDLLPVYSFNNHGVFLAKSPQRVSQPPQFLARWNELSERALADGSAA
                     PSPQS"
     gene            complement(11373..13085)
                     /gene="recJ"
                     /locus_tag="VFF22_02560"
     CDS             complement(11373..13085)
                     /gene="recJ"
                     /locus_tag="VFF22_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HZX15736.1"
                     /translation="MRIEARPLPAILPDLGSLPPLLTRLYAARGVQSAAELDKGLARL
                     IPYQQLKGMDAAVELLVQGLQQGQRMLIVGDFDADGATASTVGVLGLRMLGAAHVDYL
                     VPNRFEYGYGLTPEIVAVALEREPQLLITVDNGISSVEGVAAAKAAGLTVLVTDHHLP
                     GAELPAADAIVNPNQPGCEFPSKALAGVGVMFYVLLALRARLREMGWFTAQRPEPNLG
                     ELLDLVALGSVADVVPLDANNRILVHQGLARIRAGRARPGLRAILEVAGRDHRRITST
                     DLGFILGPRLNAAGRLDDMALGIECLLCEDEALARDMAVQLDQLNQDRKAIEQGMQRE
                     ALAQLKDLPLEDMPFGLCLFEADWHQGVIGILASRMKERYHRPTIAFADAGDGVLKGS
                     ARSVPGFHIRDALDAVAAKHPQLISKFGGHAMAAGLSLPQANFGAFAAAFDAEVRRQL
                     CEDDLTGRLLSDGALSIEEFHLPLAKELRNAGPWGQHFPEPLFHGVFQLVQQRVVGER
                     HLKVVLKSECGSVQLDGIAFGVDREIWPNPTVRWVELAYKLDVNEYRGQESVQLLIAH
                     ISPR"
     gene            complement(13244..13783)
                     /locus_tag="VFF22_02565"
     CDS             complement(13244..13783)
                     /locus_tag="VFF22_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HZX15737.1"
                     /translation="MALPSTTYKIELNLTDMDRSVYENLRFTVAKHPSETEERLAARL
                     VGYALFYHEHLAFGRGLSDVDEPALWEKSLDGRVLHWIEVGQPDSERITWCSRRTEKF
                     SLVAYGNLRVWQTKELDPVRSLKNINVVALDQEALAKLALDMPRSLNWSVMISDGELF
                     VTDERGQHELPIEWLIGER"
     gene            complement(13881..14444)
                     /locus_tag="VFF22_02570"
     CDS             complement(13881..14444)
                     /locus_tag="VFF22_02570"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZX15738.1"
                     /translation="MKTWLLVRLQLLLGIAVLMLLVQVGNSMSGYSLNIWGVIPRRLE
                     SLPGIVIAPWLHGGWAHLLSNLSGLLVLGLLALLESRRDFIKASVAIVLGSGLLVWAF
                     GREGIHVGASGWLFGLWSLLLARAWFRRSWLDLLLAVLLLLLHGGWVFGLLPQQGISF
                     EYHLAGVLCGGLYAAWRYAPRRQQQGG"
     gene            14703..15902
                     /locus_tag="VFF22_02575"
     CDS             14703..15902
                     /locus_tag="VFF22_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZX15739.1"
                     /translation="MSLPSKPLAGLKVIELGTLIAGPFASRICAEFGAEVIKVESPDG
                     GDPLRKWRKLYEGTSLWWFVQARNKQSITLNLKHEAGREVLKKLLSEADILIENFRPG
                     VLEKLGLGWDVLHALNPKLVMVRLSGFGQSGPLKDQPGFGAVGESMGGLRYITGFEDR
                     PPVRTGISIGDSIAALWGVIGALMALRHREVNGGSGQVVDVALYEAIFAMMESMVPEF
                     DVFGFIRERTGNIMPGITPSSIHTSSDGKHIQIGANGDAIFKRFMQAIGRQDLADDMT
                     LTSNDGRDARRDELYGVIDRWVGSLPLEQVLTTLNQADVPASRIFSAEDMFHDPQFLA
                     REMLLSAKLPDGKAFKMPGIVPKLSDTPGEVNWVGPELGEHNEQVLSELGYSSEQIAN
                     LRSDGAI"
     gene            complement(15930..16664)
                     /locus_tag="VFF22_02580"
     CDS             complement(15930..16664)
                     /locus_tag="VFF22_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX15740.1"
                     /translation="MKWLSKVVFLLCGAYPLAGANAATFEVGFYNYPPMMIEQGKTGI
                     YQDIFDELGKLTGDTFSVQYYPYPRIGLLFNGGQLDIEPGVYPGWVHSQPTPGVFSVP
                     FGKVVDVMMFAPGKAFPVARPEDLRGKSVGLVRGYAYPDLHALIEAGQLDRRNALNEA
                     QLLEMLAKSRFDQIVVNKAIAQYSLAKTPDYRHLEIGDVMSSFDVSMRVHPRHEAWLD
                     KLDTAILELKRRGVIEGIYAKYGVRL"
     gene            complement(16816..16908)
                     /locus_tag="VFF22_02585"
                     /pseudo
     CDS             complement(16816..16908)
                     /locus_tag="VFF22_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017150119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein L7/L12"
     gene            complement(17053..18462)
                     /gene="thrC"
                     /locus_tag="VFF22_02590"
     CDS             complement(17053..18462)
                     /gene="thrC"
                     /locus_tag="VFF22_02590"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZX15741.1"
                     /translation="MRYISTRGQAPALNFEDVLLAGLASDGGLYVPENLPRFTQEEIA
                     SWAGLPYHELAFRVMRPFVTGSIPDADFKKILEETYGVFAHNAVAPLRQLNGNEWVLE
                     LFHGPTLAFKDFALQLLGRLLDYVLAKRNERVVIMGATSGDTGSAAIEGCKACDNVDI
                     FIMHPHNRVSEVQRRQMTTILGDNIHNIAIEGNFDDCQEMVKDSFADQGFLKGTRLVA
                     VNSINWARIMAQIVYYFHAALQLGGPARSIAFSVPTGNFGDIFAGYLARNMGLPISQL
                     IVATNRNDILHRFMSGNQYVKGDLHPTLSPSMDIMVSSNFERLLFDLHGRNGAAIAEL
                     MATFKQGGGFSVDQDRWTEARKLFDSLAVDDAQTCETITEVFNECGELLDPHTAIGVR
                     AARECRRSLATPMVILGTAHPVKFPEAVEKAGVGQAPALPAHLADLFEREERCTVLAN
                     DLKTVQSFVAQHGNRGKPL"
     gene            complement(18530..>19785)
                     /locus_tag="VFF22_02595"
     CDS             complement(18530..>19785)
                     /locus_tag="VFF22_02595"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HZX15742.1"
                     /translation="TLNVLKRNAEEITRRAGRGIEVAQIAIRSPKPQYDTTGISITND
                     VFELVNNPEIDIVIELIGGYTMAKELVLKAIENGKHVVTANKALIAVHGNEIFAKARE
                     KGVIVAFEAAVAGGIPVIKAIREGLAANRINWLAGIINGTGNFILSEMREKGRTFDDV
                     LAEAQALGYAEADPTFDVEGIDAAHKLTILASIAFGIPLQFDKAYTEGITKLTTADVN
                     YAEALGYRIKHLGVARRTDAGIELRVHPTLIPADRLIANVNGVMNAVMVNGDAAGSTL
                     FYGAGAGMEPTASSVIADLVDVVRALTADPTNRVPHLAFQPDSLSDHPILPIEACESA
                     YYLRIQAKDHPGVLAQVASILSARGINIESIMQKEVEEQDGLVPMILVTHRVVEARIN
                     EAISALEALEDVVGSVVRIRVEQLN"
BASE COUNT         4033 a         6317 c         5766 g         3669 t
ORIGIN      
        1 gaacgaagtc ggcgtcgtag tgcatggctt cgtcgtcgcc agcgtccttg tcggccacct
       61 gctgcataaa gcgcgcggcc tggtcttcgg catcgttaag ctcggagtag gcgttggcga
      121 tttcgcggcc accgataaac agctcgaagc ggtcggtaac gctcggatca tcgtcgctgc
      181 gacgggccag cggcgagact tcgaacgggt agcgagtaat aaagtgcggc tgctccagct
      241 tgtgctcgac cagctcctcg aaaatcatca cctgcaactt gcccagacct tcgaagccaa
      301 gcaccttggc gccggccttc ttggcgatgg cgcgggcctt gtcgaggtca gtcaggtcat
      361 cggcggtcag ctcagggttg tacttgagga tcgaatcgaa caccgacagg cgcacaaacg
      421 gctcgccaaa gtggaacacc ttgtcgccgt acggcacgtc ggtgctgccg agcaccagct
      481 gagccagctc gcggaacagc tcctcggtca ggtccatgtt gtcttcgtag tcggcgtaag
      541 cctggtagaa ctccaacatg gtgaattcag ggttgtgacg agtcgagaca ccttcgttac
      601 ggaagttgcg gttgatctca aacacttttt caaacccacc aacaacaagc cgcttgaggt
      661 acagctccgg cgcgatacgc aggaacatct gcatatccag ggcgttgtgg tgagtctcga
      721 acggcttggc cgctgcgccg ccgggaatgg tttgcagcat cggcgtttcc acttcaagga
      781 agtcgcgctg catcagaaag ctgcggatat gcgcgatgac ctgcgaacgc acgcggaaag
      841 tctgacgcac gtcttcgttg acgatcaggt caacgtagcg ctgacggtag cgctgctcgg
      901 tgtcgctcaa gccatggtgc ttgtccggca gagggcgcag cgacttggtc agcaggcgca
      961 cgtcggtcat ttccacgtag aggtcgccct tgccggaacg ggccaaggtg ccgacggcgg
     1021 agatgatatc gcccatgtcc caggttttca ctgcggccag ggtttcttcg gacagggtct
     1081 tgcggttgac gtagacctgg atgcgcccgg tcatgtcctg aatcaccatg aacgagccac
     1141 ggttgagcat gatgcgcccg gcaaccttga ccggaatggc agcctcggcc agctcttcct
     1201 tagtcttctc ggcgtactgt ttctgcaggt cggcgcagta gttgtcacgg cgaaaatcgt
     1261 tggggaaggc gttgccctgc tcacggatgg cggcaagctt ttccttgcgc tgggcaacca
     1321 gcttgttttc ttcctgctgc tggtcttcgt gggagagggc ttgctggtcg agttgttggt
     1381 cgctcatggt cgttcgtctg tcctgtaaat cgttggcgga tggttgggtc gcgcatggcg
     1441 cacgcccaac ctgcctaaaa ccatcactcg gcggttagag cccctgcttg aggctcgcca
     1501 ccaggtattc gtcgatatcg ccgtcgagca ccttgtcgca gtcgctgcgc tcgatgttgg
     1561 tgcgcagatc cttgatccgc gaggcatcaa gcacgtagga gcgtatctgg tgaccccagc
     1621 cgatatccga cttgctgtct tccagcacct gcgaggcggc gttgcgcttc tgcacttcct
     1681 gctcgtacaa acgcgcccgc agcatcttca tcgcggtgtc tttgttggcg tgctgcgaac
     1741 gttcgttctg gcaactgacc acggtgttgg tcggcacgtg ggtaatccgc accgccgaat
     1801 cggtggtgtt tacgtgctga ccaccggcgc ccgatgaacg ataggtgtcg atacgcaggt
     1861 ccgccgggtt gatctcgatt tcgatgttgt catcgatttc cggcgagacg aacactgcgg
     1921 taaacgaggt gtggcgacgg ttgccggagt cgaacgggct tttgcgcact aggcggtgca
     1981 cgccgatctc ggtgcgcagc cagccaaagg cgtactcgcc tttgatatgc agggtcgcgc
     2041 ccttgatccc ggccacttcg ccagcggaca gttccatgat ggtggcgtcg aaaccgcgct
     2101 tgtcggccca gcgcagatac atgcgcagca gcatattggc ccagtcctgg gcttcagtac
     2161 cgccggagcc ggcctggatg tccaggtagg cgttgttcgg gtccatctcg ccgctgaaca
     2221 tacggcggaa ttcgagcttt tcgaggatct cacgcaggcg ctcgatctcg gcggcaacgt
     2281 cattcaccgc gccttcgtcg tcttcttcga cggccatatc gagcaggtcg cgcgaatcgg
     2341 ccaggctgcc gtgcaggtca tccagggtat cgacgatcag cgccagcgag gcgcgctcgc
     2401 gacccagatt ctgcgcgtat tcggggttgt tccagacgtt ggggtcttcg agttcgcggt
     2461 ttacttcgac gagacgatca tgtttgtgat cgtagtcaaa gatacccccg aatagtcagg
     2521 gtgcgctcgg acaggtcctt gatgctatta aggatcgggt tgatttccat ggctggcagc
     2581 actcgtaggt caaactttcg gaaaagccgg cgagtatagc ccaatcgaat cggcccttgc
     2641 agcccacaat tggcgctggc gcacaacagt gcagaccaac ggccactatg ctcaagcgca
     2701 cctgtacact gcctgcccgc ccacaggagc cgtcgtgcca tgagttcatc gcacagcctg
     2761 cgtagtcgtt tcgccatttt gattgccctt cttgtcgcca gcctgagctg gttgctcggc
     2821 accctgattg gccacgacgc cagccagcgc atccgcgagg aagtcggccg cgacctcgcc
     2881 gaagtctctt ttatgatggt tgaccgcctg gatcgcgaca tgcagagccg cgcagccatg
     2941 ctgcaggtga tcggcaaact gcgtgcactc aaccagccgg acgatatcgt cgaggtccgc
     3001 gtgctgctcg accgcctgca gatcgagttc cccagcatcg cctggatcgg ctttaccgac
     3061 gccaaaggta cggtactggc ctccagcgac ggtgtactgg aaggtgccag catcgcccag
     3121 cgcccggtgt tcctggaagc gcagaaaaac gtgtttatcg gcgacgtgca tgaagccgta
     3181 ctactggcca aattgctgcc taacccaacc ggtgaggcga tgaagttcgt cgatatcagc
     3241 ctgccggtat ttgctcccga cggcagcctg gtgggcgtac tggcctcgca cctgtcctgg
     3301 tcatgggcag acgaagtgcg cctggccatc ctcgagccga tccagaagcg ccgtaatgtc
     3361 gagttcttcg tcatcggtga agaccgcacc gtgctgctcg cgcccaagga gctgatcggc
     3421 cagcgcctgc atctgcccgc cctcaacgac gtggcgcaac gcaaggacct ctgggccgtg
     3481 caggagtggc cagacggcaa gcaatacctg acgggcatgg cactaagcca aggccatatg
     3541 gactacaagg gcttgggctg gaccgtgatc gcccggcaaa atctggatga agcctacgct
     3601 ccggcgcgcg aaaccacgcg ctatatcctg ctgtggggta gcggcctggc gctattgttc
     3661 gccctgctcg gctggctact cgccggctat ttcacccgcc cgctgcggca gatcgctgag
     3721 gctgcggacc gcctcagcgc cggtgaaatc agcgtgatcc ccgagctgaa aagcacccgc
     3781 gagatcgaga tactcagcaa ctcgattcgc cacctggtgg aaagcctgac ccaccagcaa
     3841 aacgccctcg gagcgatgga aaacctcgcc caccacgacg cgcttaccgg tctgcccaac
     3901 cgtgtcgccc tggaaagcta cctgccgcgt gcgcaacaac gcagccagag caataacaat
     3961 tgcctggcgc tgttctatct agatctggac ggtttcaaac cgatcaacga ccagttcggc
     4021 catgcagcgg gcgatcagct gttacgtgaa gtggcggtgc gcctgcgcgg ctgcctacgt
     4081 gaaggtgact tggtcgcgcg cctgggcggt gacgaattcc tgatggtgct ccaggtaccc
     4141 agcagcgaag ccctggggca agcgcagctg gtggccgaac gcactcttgc ggcgctgagt
     4201 gcgccggtcg acctgggcaa caatcaggtc aaggtcggtt gcagcatcgg cggcgcgctg
     4261 tggccgctgg acagcagcaa cctggccgaa gcgctggagc tggccgacca ggcgctttac
     4321 cgagccaagc atgcgggcaa gaactgcgtg atatttcata gtcaggctga cgcagacggt
     4381 taattaccgc gctcgggcac cggcaccgca actaggtaga cgcgaggatc aattgcgccg
     4441 ccttctccgc gatcatcaat actggcgaac aggtattgcc agagacgatg ttcggcatga
     4501 tcgacgcatc ggcaatccgt agccccggca caccatgaac gcgcaattgc gcatcgacca
     4561 ccgcatcggc gccagcgccc attttgcagg tgccggctgg atgaaaaatg gtggtgccga
     4621 tctcgcccgc cgcgcgctga aggtcttcct cgctctgaca actgggccca ggcttgtatt
     4681 cttcaggcct gaacggcgcc agcgctggcg cagcaacaat gcgacgggtt aagcggatag
     4741 catcggccgc cacctgcaag tcgcgagtat cacttaggta attgggttga atcagcggcg
     4801 tttcggcgct gttcagcgaa cggatttcga ccttgccacg gctgtacgga cgcagattgc
     4861 agaccgaggc ggtaaacgcc ggaaacgcat gcaacggttc gccgaaacgc tccaatgaca
     4921 gcggttgcac gtggtactgc agattggccg acggctgttc ggcgcaggag cgagcgaacg
     4981 cccccaattg gctcggcgcc atggacaagg gcccgctgcg gctgagcagg tactcgacgc
     5041 ccatgcccat cttgccccac aagctacccg cgatctggtt cagcgtcggc acctcgctga
     5101 ccttgtaaat caggcgcaac tgcaagtgat cctgcaggtt ctcaccaacg ccagccagct
     5161 catgctgcac accgatacct aagcgctgca gcaagctgcg cgggccgatc cccgagcgtt
     5221 gcagcagcgc cggcgtacca atggcgccgg cgcataagat tatttcgcgc tgggccttga
     5281 tggtccgatc ctgcccttgc cagtgcaggt taagcgcttc agcgcggccg ttgccaagga
     5341 ctacccgctg cacgtcggca tcagtcaaca cacgcagatt ggggcgttta cgaatatccc
     5401 gaagaaaggc cttggatgca ttccaacgca caccacggcg ctggttgacc tggaaataac
     5461 tgcagccttc gttatcacct gtattgaaat catcgatgct agcgatgccg ctctgcgccg
     5521 cgccctggcg aaacgcttca agcaaatccc aggtgagccg ctgctgttcg acccgccact
     5581 cacccgtccc gccgtgcagc gcatcgccgc cgccatagtg gttttccgtc cgtttaaaca
     5641 gcggcagcac atcctgccag gcccaaccgg gattgcccgc ggcggcccag ccgtcgtagt
     5701 cgcgcgcctg gccacgcatg taaatcatgc cgttgattga tgagcagcca cccagcacgc
     5761 gcccgcgcgg gtatttcaat gaacgcccgt tcaggcctgg ttcgggcgcg gtgtcgtagc
     5821 accagtcggt acgtgggttg ccgatgcagt acaggtaacc aaccggaatg tgaatccatg
     5881 gatagttgtc gcgccctccc gcctccagca gcagaacgcg gattgccgga tctgcggaca
     5941 gtcgattggc cagcaggcag ccggcagggc cggcaccgac gatcaggtaa tcgtattcct
     6001 cttcaagttg ctgcatcggc atctcgctat gaccatcgcg gtttgaatgg acaacagcct
     6061 agccgcactg catgcaagct ggacggtctt ttagtccgag ataaatatgc tgaatgattg
     6121 gctcaaggcg gcgacaactc ggccgtgctt accgcgagcc aaggaccgga ggaattgcca
     6181 gcgagggccg accagtacca ggccgagtta tcgctgaagc gttttccatt ggcatcctcg
     6241 atgtactcgc acacgcgcag cttcgcccca tgcccactgg caataaagca gcgttgcaaa
     6301 cctgctgcgt cggtgcgctg ctccagcacc atggcctgaa tgcgataacc cagaccgcgg
     6361 taacaatccg ccgcggggtg cagtttacgg gttgggcgca tgacatgacg caaggtgact
     6421 acccgtgcgc ccgcgctgaa acgcgcgata gcgccgggga attgctcggc aaaacgctgc
     6481 tcgaccgctg acaacgccaa cggccgcaat gcatcgccgg cgtactgctg cggccattcg
     6541 acgaagttgc cgtggcgcgg ctcgctcgct atcggcttag ccagcatcgg ccacagcgcc
     6601 aacagggcga acgacagcac cagcaagcgc tgcataacag gatcgattgg tttgagcggc
     6661 cggcagcaca cgggctcggc aaccatctgt gcgcgtgtac gccccgctgc tacgtaacgc
     6721 aggaccagcg tgcaaacgcc gacgaacacc agcaagccgg tgccctcgtg catccagccc
     6781 ggccaatcca aacgcccagt ttcctgcaac accagcaggc tgttacgcag gatattgccg
     6841 gccagcacca gcaccccgag caccggcaaa cgccttagca gctgccggtc gctcagccgc
     6901 agccaggcgg ccgtggtgaa agcagtgaaa taggcgaccc aggccatctg gatacccgaa
     6961 cagggcgcat cgaccatcac caattgcccc gccacttcta gtgtgctgcc ctggcgcagc
     7021 acctccagcc caccgctgcg taacaaccag gcgctgacct cggcggtcag cactcgcagc
     7081 ggatagccga taaagaattg caaggacgac ataatcggca gcgccagcaa acccagcccc
     7141 aaccaggcca gcaacgcctg accggacgcc cgcagggcca gtacgacagc gaatagggtc
     7201 aataccgcca gcaccccgcg caacaatggc ggtaatcctg gcgccaatgc ggtcgccgca
     7261 cagagcagca atgccagcag caaccaaggc agtcgcgcct ccgccgataa tcgccggcgc
     7321 tcgcgccaga tcatcagcag cagcgcagcc aaggcgacga tgccaaacgg atcgtccgaa
     7381 ccatcgctca tccgttgcgc gctccagcgc cagaccggcc acagcgccag agtcggcaac
     7441 agcaaccaca gccagcccgg tggatgcgca gcggcgcgta atcggccggt cctcagccat
     7501 gccatcattc agttctgctc ctggcgcgca tgctgacgcg ccagccagcc catacctaga
     7561 gctgcgacca acaacatcag ccaggtgctc ggctcggggg tgctcgatac cacggcacca
     7621 atggcctggt tgctcacttc ttggggcagc ggtagcgtct gtttgacgct gtcggcagca
     7681 gccggatctg ggttgcgcac ctgttcgtcc accgcgataa acgaggtgta ggccgtcagc
     7741 aggctgtact tcaagcccag gtcgagaatc gcctggctat aggtgtaacc gccttccagg
     7801 gtttcctgat cgctcaggct ggcgacctta tgccgcgccc ataaatagcc cagcgcccga
     7861 ttaccttcgc tggccatctg cgattctatc ggcacctcaa ctcggtaggg cccggccgca
     7921 ccgtgacctt ccacgagcag gttgccacgc gcctcgccgc gccacttgcc gaacacaacc
     7981 agcggccgct gggcaaacag atccggcagg gccacgggct caacgtcata aacctccagc
     8041 ccgtcgaaac gcacctgcgg attggccaac accggtgcat caatcatctg ccgaaagcgc
     8101 tcggcctgct catccgccga tgccttgttg agaatcacga agggctcgcc ctgcccggcc
     8161 cgggccaggc cctcgatcag ttggcgattg accgacttgc cgataccgaa ggcgaacagg
     8221 ttggccttgt ccaggttatt gcgcaccagc tcgaaggcct cgcgctccac ggtcacatag
     8281 ccatcggtga ccaccacgaa gctgcgcgcc cgctcgggat cggccggcac cgccagggct
     8341 tggcgcaatg ccggcaacag ctcggtgccg cccgacccca tcgtggtatc gagcatgtcc
     8401 aaagcccgct caatattcgc cggcgtggcc gtctgtgact cggcggcgag catgctgcta
     8461 ctgccggaga acagcagcac attgaagctg tcggaggggc gcaaagcacc gaccagattc
     8521 ttcaaaaggt gcttggcggt atctaaagga aagccatgca tggagccaga aatatccacc
     8581 acgaagatat actcgcgcgg caccagcatc tggcttttca ccttcgccgg cggggcgatc
     8641 aaggccagaa agaagttctc gtcgacaccc tttgacagca gcacaccgct ttcgaaactg
     8701 gcgccgctca agcgatagtc aaggacgaaa tcgcggttat tgctctgccc ttcctcggcg
     8761 ccgagactga ccagcgcctg actctgcatg atctgttgca actcaatgcg atggctcggc
     8821 gagcggatat cgaccaacgg taccggcgac tgcaactcaa ccttaagcgc aaagctatcg
     8881 cgcgtcgttt gcccctctgg caaattaggc gtgctaaccc agggcccgcc cttgccgctc
     8941 tcgctacccc ccgcgccgtt gtagcgcggc ccaaccacgg tagggaaaac gaagctgtag
     9001 acgccatcgg tgggcagcag caactcggta taacgcagct ccacatcgac cacatcgcca
     9061 ggcaggatat tggcgacgtt catctggaat acgttttcgc ggtgctgttc gagcagtgct
     9121 gccgttttgc ccatgctttt ggcctgctga tattcctgct gggccttctg cttctcgcga
     9181 atttgcgcct gcagttcacg atcgcctaga cgcacggtca tgccgtacac cgccgcgcgg
     9241 gacgaacctg gaaacacata acgggcttcc agcgcacgcg taccttcgtt gcggtattgc
     9301 tggacgaccc gcacatcgac aatcacaccc agtacccgca cctcgacctg ggtggatttg
     9361 agcggcaaac gatcaacgct cggatcgtcg ctctgcacca cgaaataagg gctttcactg
     9421 cgctctactg cagattgcgc acaggtcagc gcgggttgcg atagcagcaa ggcgaacagc
     9481 aacgcaagcc ggcggccgac gcgggttctg ctggtgaaca aagaatgaaa catgacaaag
     9541 ctccccgtga gttatgcaaa cacgggggct atgttggagt tgagatgtgg tgggagtttg
     9601 gggctgcggt ggcgattgtg tggagttttg catccacaca tttaagtggc tcgaggatta
     9661 gctaacgtcc agctgcaacc tcacctgggc ttccagttcg ctcttcagcg ccgggtccag
     9721 cttgagctga cgggccagct cttccaggta agcgcgctcc ataaagtgct cttcatcgac
     9781 catcagtacg ctggcgatat acatttctgc agccatttcc ggcgtgctgg ctgcgcgagc
     9841 gatttcggcg ggatctagcg gtttgttcag ttcagcctgt agccaatgct gcagttcgcc
     9901 gtcactggtg agtttgacca actcgccttc gatcaactgc cgctcgcggg catcgacatg
     9961 gccatcggct ttggctgcgc cgaccagcgc cttgagaatc gcttggctgt gcagctcggc
    10021 ctgcggtgcc ggcaagcgat ccagggtttg tggctcggtc tgtggcgcat tggcctgctg
    10081 cgcttgccag ttgctgtaag ccttgtaggc aaccacgcca agcgcggcca ggccaccata
    10141 ggtcagcgcc ttgccgccca tcttgcgcgc actcttgttg cccagcagca tgcccagcgc
    10201 accggcggcc agcgcgccac cacccgcccc cttgagcaga gtgccgagat caccgccctg
    10261 gccaccttta ccggcgccgc cgagcaaacc gcccagagca ccacccaggc caccggccga
    10321 gccaccagcg gaactgccgg cggatttctg ctgcatcagg tcctggccgg atttcaacaa
    10381 ctgatcaagc aagccacggg taatcatcaa gcgctctccc taaaaatagt tgtgcaagca
    10441 cacagactga ttctagagac ggaggtttct caccacctaa cgccagagtg cttcgaaaga
    10501 ttgccagcgg acgtgaaagc caggaaaacc ggttatccgt cggctttctg ccggttacag
    10561 gactgcaaat atgtcaaata gtggtcacca tcacactctg ccgatggtcg atctatattt
    10621 gacgccgata ggatgacgga cacgaacctc gcaacggcaa aaggctatca agagccaacg
    10681 ccggagacct tgcgccatgc cacaacgcgc caatcgcccc gctcaccgcc tgctgcgcag
    10741 cctgcttgcc agttgctggc tattcagccc ggtgctggtg gccgatgcgc cgagcactca
    10801 acacagcgcc ctggctacgg ctctcagcga tacgcgcttg gccagttggc tcaacctgct
    10861 cgacaactcc gctggcgaca acgaacgcgc ccagctggag cgggtcaacc acttcatcaa
    10921 tcgttcagtg agctttgtca gtgaccagca ggcttgggga gctgaggatt actgggccac
    10981 accggcgcaa acacttagcc taggcaaagg tgactgcgaa gatttcgcca tcgccaaata
    11041 cttcagcctg gtgcgcatag gcgtgccgag cgagaaactg cgcctgactt tcgtcaaagc
    11101 gctcaagcag aaccaagcgc atatggtgct ggcctattac cccagcgcca gcgagcaacc
    11161 gctgattctc gacaacctcg acccgcgcat ccgcccggcc agtgagcgtg gcgatctgct
    11221 gccggtgtat tcctttaata accacggcgt gtttctggcc aagtcgccgc agcgtgtcag
    11281 ccagccaccg caatttctcg cccgctggaa tgagctgagc gaacgggccc tggccgatgg
    11341 ctcagccgcc ccgtcaccgc agagctagcc aatcagcgcg ggctgatatg ggcgatcagc
    11401 aactgcacgc tttcctgccc acggtactca ttgacgtcca gcttgtaggc cagctcgacc
    11461 cagcgcacgg ttgggttcgg ccagatctca cgatccacac caaacgcgat gccatcgagc
    11521 tgtacgctgc cgcattcgct cttcagcacc accttgagat gccgctcgcc gaccacgcgc
    11581 tgttgcacca gctgaaatac accatgaaac agcggctcag ggaaatgctg gccccagggc
    11641 ccggcattgc gcagctcctt ggccaacggc aggtggaact cttcgatgga caaggctcca
    11701 tcggacagta aacgcccggt tagatcgtcc tcacacagct ggcggcggac ttcggcgtcg
    11761 aaggcagcgg caaaagcacc gaaattcgcc tgcggcagtg acagcccagc ggccatggcg
    11821 tgaccgccga acttgctgat cagctgcgga tgtttggctg ccaccgcgtc cagcgcatcg
    11881 cggatatgaa agcccggaac cgagcgcgcc gaacccttca gcacaccatc gccagcatcg
    11941 gcaaaggcga tggtcgggcg gtgataacgc tctttcatcc gcgaggcgag gataccgatc
    12001 accccctggt gccaatccgc ctcgaacagg cacagaccaa agggcatgtc ctccagcggc
    12061 aggtccttga gctgggccaa agcctcgcgc tgcataccct gctcgatggc cttacggtcc
    12121 tgattcagct gatcgagctg caccgccata tcgcgggcca gcgcttcgtc ttcacagagc
    12181 aggcattcga tgcccagcgc catatcatcc agccgcccgg ccgcattcag ccgtgggccg
    12241 aggataaaac ccaggtcggt ggaggtgatg cgccggtgat cgcgccccgc cacctcaaga
    12301 attgcgcgca agcccggccg cgcccggccg gcgcgaattc gcgccaggcc ctgatgcacc
    12361 aggatgcggt tgttggcatc cagcggcacc acgtcagcga cgctacccag ggccaccaga
    12421 tcaagcagct cgccgagatt tggctccgga cgctgcgcag taaaccagcc catttcacgc
    12481 aagcgagcac gcaacgccag caacacgtag aacatcacgc caacccccgc caacgcctta
    12541 ctggggaact cacagcctgg ctgattggga ttgacgatgg catccgctgc cggcaactcg
    12601 gcgccaggca ggtggtgatc ggtaaccagc acggtcaaac cggccgcctt ggccgcagcc
    12661 acaccttcaa cgctggagat gccgttatcc acggtgatca gcaattgcgg ctcgcgctcc
    12721 agcgccaccg caacgatctc cggggtcagg ccgtagccgt attcgaaacg attgggcacc
    12781 agataatcga catgtgcggc gccgagcatg cgtaacccca gcacccccac cgtactggcg
    12841 gtggcaccat cggcatcgaa gtcgccgacg atcagcatgc gctggccctg ctgcaagccc
    12901 tgcaccagca gctcgactgc cgcgtccatg cctttgagct gctgatacgg aattagccgc
    12961 gccaggccct tatccagctc agccgccgat tgcacgccgc gggcggcata cagacgggtc
    13021 aacaacggcg gcaggctgcc cagatcgggc aggatggcgg gtaacggacg ggcttcgata
    13081 cgcatggtta ttctttttcg tgtagggagg gtgcaacccg ccgaaatcaa aacgtagcgg
    13141 ccattcaaag acgtagcccg gatgcaatcc ggggttatcg ggtctcaaca gcccccggat
    13201 ttcatccggg ctacgggaac tgtctcccgc ataaagcagc tacttaacgt tcgccgatca
    13261 gccactcgat cggtagctca tgctggccac gctcgtcggt gacgaacagc tcgccgtcgc
    13321 tgatcatcac gctccagttc agcgagcgcg gcatatccag cgccagcttg gccagggcct
    13381 cctgatccag ggcgacgacg ttgatatttt tcaggctgcg caccgggtcc agctccttgg
    13441 tctgccacac ccgcaggttg ccgtaagcca ccaggctgaa cttctcggta cgccgcgagc
    13501 accaggtgat gcgctcgctg tccggctggc cgacttcgat ccagtgcagc acgcgaccgt
    13561 ccaggctctt ttcccacagc gccggctcat ccacatccga caggccacga ccgaaggcca
    13621 gatgctcgtg gtagaacagc gcatagccaa ccagccgcgc ggccaggcgc tcttcggttt
    13681 ccgaggggtg tttggccact gtgaaacgca ggttttcata aacgctgcgg tccatgtcgg
    13741 tgaggttcag ctcgatttta taggtcgtgg aaggcagggc catgatgggg cttcttggct
    13801 gatcgaataa gccggcaagt ctaccgcaaa ggccgcctcg ccacgaaccc gagtgctcga
    13861 tggagcgcag ccaagcccag tcaaccgccc tgctgttgcc gccgcggcgc atagcgccag
    13921 gccgcgtaga gccctccgca cagtacgcca gccagatggt attcaaagga aataccctgc
    13981 tgcggcaaca gaccgaacac ccagccgccg tgcaacagca acagcaggac cgccagcagc
    14041 agatcaagcc agctgcggcg aaaccaggcc cgcgccagca acaacgacca gagtccgaac
    14101 aaccagccgc tggccccgac atgaatgccc tcgcggccga acgcccagac cagcagacca
    14161 ctgccgagca ctatcgccac actggccttg ataaagtcgc gccgcgattc gagcagcgcc
    14221 agcagtccca gcaccagcag cccgctgaga ttgctcagca agtgcgccca gccaccatgt
    14281 agccagggcg cgatgacaat acccggcaag gactcgagcc gccgcggtat taccccccag
    14341 atattcaggc tgtaaccgct catgctgttg cccacctgca ccagcagcat cagcacggca
    14401 atgcccagca gcaactgcag gcgtaccagc agccaggttt tcatggtcgc tcaccggcca
    14461 tacaatccgg ccgcgacggg ctggacaggt gccgatacag gcagggctcg agggtcatca
    14521 tcgtgtttag cgcatccatc agggcagtaa gccgttatgc cggatatcgg ccgggctttc
    14581 cagagggcag attgcagcgg ctcggccact gcggccggtg ccttgccgca accgcctgga
    14641 gcaatagccc aaccgcgccc aatgcaatag gcttagccgc gcaacccagc ccaggagaca
    14701 gcatgtcctt gcccagcaaa cccctcgccg gcctgaaagt gatcgagcta ggcaccctaa
    14761 tcgccggccc gtttgcttcg cgcatctgcg ccgagtttgg cgccgaggtg atcaaggtcg
    14821 agtcgccgga tggcggtgac ccgctgcgca aatggcgcaa gctgtatgag ggcacttcac
    14881 tgtggtggtt tgtgcaggcg cgcaacaagc agtcgatcac ccttaacctc aagcatgagg
    14941 ccggacgcga ggtgctgaag aagttgttga gcgaagccga tattctgatc gagaacttcc
    15001 gtcccggcgt gctggagaaa ctcggcctgg gctgggatgt attgcatgcg cttaacccca
    15061 agctggtgat ggtgcgcctg tccggcttcg gtcagagcgg gccgctgaag gatcaaccgg
    15121 gcttcggcgc ggtgggcgag tccatgggcg ggctgcgcta tatcaccggt ttcgaggacc
    15181 gtccgccggt gcgcaccggc atctcgattg gcgattcgat tgccgcgcta tggggcgtga
    15241 tcggcgcact gatggcgctg cgtcaccgcg aagtgaacgg cggcagcggc caggtggtgg
    15301 acgtggcgct gtatgaggcg atcttcgcca tgatggaatc catggtgccg gagttcgatg
    15361 tgttcggttt tatccgcgaa cgcactggca atatcatgcc cggcatcacg ccctcctcga
    15421 tccacaccag cagcgacgga aaacatattc agatcggcgc caacggcgat gcgatcttca
    15481 agcgctttat gcaggccatc ggccgccagg acctggccga cgacatgacg ctgaccagca
    15541 atgatggccg tgacgcgcga cgcgatgagc tgtatggcgt gatcgaccgc tgggtcggct
    15601 cgctgccgct ggagcaggta ctgacaaccc tgaaccaggc cgacgtaccc gccagccgca
    15661 ttttcagcgc cgaggacatg ttccatgacc cgcaatttct cgcccgagaa atgcttctgt
    15721 cagccaagct accagacggc aaagccttca agatgcccgg catcgtgccc aaactgagcg
    15781 acacaccggg cgaggtgaac tgggttggcc cggaactggg cgagcacaat gagcaggtgc
    15841 tgagcgagct gggctatagc agtgaacaga tcgccaatct gcgtagcgat ggcgccatct
    15901 aatcagccat gcctgcgggc gcaagcctgc tagaggcgaa cgccatattt ggcgtatatc
    15961 ccctcaataa caccgcgccg cttgagctct agaattgccg tatccagctt atccagccag
    16021 gcttcgtggc gtggatgaac gcgcatgctg acatcgaaag agctcattac atcgccgatc
    16081 tcgagatgac ggtaatccgg ggtcttggcc aggctgtatt gagcaatcgc cttgttgacc
    16141 acgatttgat caaagcgcga tttcgccaac atctccagca gctgcgcttc attcagcgca
    16201 ttgcgccgat caagctgccc cgcctcaatc agcgcgtgca ggtcagggta agcgtaaccg
    16261 cgcaccagcc ctactgactt gccacgcaaa tcctcaggcc ttgccaccgg aaaagccttg
    16321 cccggagcaa acatcatgac atcaacaacc ttgccgaacg gcaccgaaaa caccccaggg
    16381 gtcggctggc tgtgcaccca acccggatag acacccggct caatatcgag ctgcccgccg
    16441 ttgaacagca agccaatacg cggatagggg tagtactgca cgctgaaggt gtcgcccgtg
    16501 agcttgccta gctcatcgaa gatgtcctga taaataccgg ttttaccctg ctcaatcatc
    16561 atcggcgggt agttataaaa accgacttca aaggtagcgg cgttagcgcc agccaaggga
    16621 taggcaccgc atagcagaaa aaccaccttg ctcaaccact tcacgccatc accctactac
    16681 ccctaccgca atgcactgcc gctagaacgc gccaaacaac caggcgactg cggcgcccag
    16741 cacaagtaag cccaacaacc agaagatacc gccatgcccg cccgactgca ctaccgagac
    16801 gcctcccgac tgtcgctcag gcgtgtagcc gtccacggcc tccttggcct ccttcaagcc
    16861 aatccccttg agcttacgca gcagcttgat tgcctcgatt ttctgcccac gctcaagtgc
    16921 cgctatcacc tccaccggca aatcctgaac aaactcagcc atgaaacgct ccagcccaac
    16981 aaactaccca gagcatagcc gcaaaaaaag ccggcccctt gcgggtaccg gctttgtcga
    17041 ccagcgcctg gtttacagcg gcttgccgcg attgccgtgc tgagccacga aactctgtac
    17101 ggttttcagg tcattggcca gcaccgtaca gcgctcctcg cgctcgaaca gatccgccag
    17161 gtgcgcaggc agcgccggtg cctgaccaac accagccttc tctaccgcct cagggaattt
    17221 caccgggtgc gcggtaccaa gaatcaccat cggcgtagcc aggctacgac ggcactcacg
    17281 ggcagcacgc acgccgatcg cggtatgcgg atcgagcagc tcgccacact cgttaaacac
    17341 ctcggtgatg gtttcgcagg tctgcgcatc atccaccgcc agcgagtcga ataacttgcg
    17401 cgcctcggtc cagcgatcct gatcgacgct gaaaccgccg ccctgcttga aggtggccat
    17461 cagctcggca atcgccgcgc cgttgcgacc gtgcagatca aacagcaggc gctcgaagtt
    17521 ggacgagacc atgatgtcca tggacggcga cagggtcggg tgcaggtcgc ccttcacata
    17581 ctgattgccg ctcataaagc ggtgcaggat gtcgttgcgg ttggtcgcga cgatcagctg
    17641 gctgatcggc aggcccatat tgcgcgccag gtaaccggca aaaatatcgc cgaagttgcc
    17701 agtcggcacc gaaaacgcga tggagcgcgc cggaccaccc agctgcaaag ctgcgtggaa
    17761 gtagtaaacg atctgggcca tgatccgcgc ccagttgatc gagttgaccg caaccaggcg
    17821 ggtgcccttg aggaaaccct ggtcagcgaa gctgtccttg accatttcct ggcagtcatc
    17881 gaagttgcct tcgatggcga tgttatggat gttgtcgccg aggatggtgg tcatctgccg
    17941 gcgctgcacc tcggacaccc ggttgtgcgg atgcatgata aagatgtcga cgttgtcgca
    18001 ggccttacag ccttcgattg cagccgagcc ggtatcaccg gaggtcgcac ccatgatcac
    18061 cacgcgctcg ttgcgcttgg ccagtacgta gtcgagcaaa cgaccgagca gctgcagggc
    18121 aaagtccttg aacgcaaggg tcgggccatg gaacagctcc aacacccact cattgccatt
    18181 cagctgacgc aggggtgcca cggcgttgtg ggcgaacacc ccgtatgttt cttcgaggat
    18241 tttcttgaag tccgcatccg ggatgctgcc agtgacgaac gggcgcatca cccggaacgc
    18301 cagttcgtgg tacggcaggc cggcccagga agcaatttct tcctgggtaa agcgcggcag
    18361 gttttccggt acatagaggc cgccatcact ggccaggccg gcgagcagca catcttcgaa
    18421 attcagggcc ggtgcctggc cacgggtact gatatagcgc ataggggcaa accttcggtt
    18481 gaatctttaa gggccgcagc tgcctgctgc acctgcggcc agaggcggat tagttcagct
    18541 gctcgacgcg gatacgcaca acgctgccga ccacatcttc aagcgcttca agggcactga
    18601 tcgcctcgtt gatccgcgct tcgaccacac gatgggtgac caggatcatc ggcaccaggc
    18661 cgtcctgctc ttcgacttcc ttctgcatga tcgattcgat attgatcccg cgggccgaca
    18721 gaatgcttgc cacctgggcc agcacacccg gatgatcctt ggcctgaatg cgcaggtagt
    18781 aggcgctctc gcaggcttcg atcggcagaa ttgggtgatc ggacagcgag tctggctgga
    18841 aggctaggtg cggcacacgg ttggttggat cggccgtcaa ggcgcgcacc acatcaacca
    18901 gatcagcgat gaccgaggag gcggtcggct ccatgcctgc accggcaccg tagaacagcg
    18961 tgctgccagc ggcgtcgcca ttgaccatca ccgcgttcat cacgccattg acgttggcga
    19021 tcaggcgatc agccggaatc agcgttgggt gtacacgcaa ctcgataccg gcgtcggtgc
    19081 ggcgggccac acccaggtgc ttgatgcgat agcccagcgc ttcggcgtag ttcacgtctg
    19141 cggtggtcag cttggtgatg ccttcggtgt aggctttgtc gaactgcagc ggaataccaa
    19201 atgcgataga cgcgaggatg gtcagcttgt gcgcggcgtc gatgccttca acgtcaaagg
    19261 tcggatcggc ctcggcataa cccagcgcct gcgcctcggc cagcacgtcg tcgaaggtgc
    19321 gacccttctc gcgcatttcg ctgaggataa agttgccagt gccattgatg atcccggcca
    19381 gccagttgat gcggttggcc gccagacctt cacgaatagc cttgattacc ggaataccgc
    19441 cagcaaccgc ggcctcgaag gcgacgatca cgcccttttc gcgagccttg gcgaagatct
    19501 cattgccatg cacggcgatc agcgccttgt tggcggtgac cacgtgcttg ccattctcga
    19561 tggccttgag caccagttcc ttggccatgg tgtaaccgcc gatcagctcg ataacgatat
    19621 cgatctcagg gttgttcacc agctcaaaca catcgttggt gatgctaata ccggtcgtgt
    19681 cgtactgcgg cttgggcgag cgaatggcaa tctgggcaac ttcaatacca cgccctgcgc
    19741 gccgggtaat ttcctcggca tttcgcttga gtacattcaa cgtac
//
LOCUS       DASRUM010000033         4217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1523397, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000033 DASRUM010000000
VERSION     DASRUM010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523397"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            470..1414
                     /gene="puuE"
                     /locus_tag="VFF22_02600"
     CDS             470..1414
                     /gene="puuE"
                     /locus_tag="VFF22_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HZX15743.1"
                     /translation="MSADYPRDLIGYANNPPHPHWPNDARIALSFVLNYEEGGERNIL
                     HGDKESEAFLSEMVSAQPLQGQRNLCMESLYEYGSRAGVWRLLNLFQKHNIPLTIFAV
                     AMAAQRHPDAIKAMVAAGHEICSHGYRWIDYQNMSEAEEREHMLEAIRILTELTGQRP
                     QGWYTGRTGPNTRRLVREEGGFLYDSDTYDDDLPYWDPASTAAKPHLVIPYTLDTNDM
                     RFTQVQGFNKGDDFFEYLKDAFDVLYAEGVAGAPKMLSIGMHCRLLGRPARLASLAKF
                     LEYVQSHEKIWCARRVDIAQHWYATHPLHDQPSKESSK"
     gene            1411..1926
                     /gene="uraD"
                     /locus_tag="VFF22_02605"
     CDS             1411..1926
                     /gene="uraD"
                     /locus_tag="VFF22_02605"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HZX15744.1"
                     /translation="MSRFQTLTPSRLNREDFVKVFADIYEHSPWVAEKAFDLDQDDSI
                     NNIDGLHQRMADLLLSASHAAQLELINAHPDLAGKAAVRGELTASSTAEQAGAGISEC
                     TADEFARFTELNDAYKAKFGFPFIMAVKGSNRHQILAAFEERIHNSPEQEFACALAEI
                     NKIALFRLQQL"
     gene            1975..2970
                     /gene="alc"
                     /locus_tag="VFF22_02610"
     CDS             1975..2970
                     /gene="alc"
                     /locus_tag="VFF22_02610"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020299584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HZX15745.1"
                     /translation="MKAYAVPFEKYVNLADARLGTQAISVTDDWFADVNRLFQPTPAV
                     WKEGVFDDNGKWMDGWESRRKRFEGYDSAVIRLGVPGSIKGVDIDTSFFTGNFPPSAS
                     LEGCFVAEGDPTDATEWTEVLAAVELQGNSHHYHEIATAQAFTHLRFNIYPDGGVARL
                     RVYGVQFRDWSNVGDNEQIDLAAALNGGRALACSDEHFGRMSNILNPGRGINMGDGWE
                     TARRRTPGNDWVIVALGHPGEIERIVVDTLHFKGNYPDSCSIQAAFVKGGTDSQIETQ
                     SLFWRELLPSQKLQMHAEHEFSEQIKALGPITHVRVNVFPDGGISRLRLFGKVAK"
     gene            3030..3533
                     /locus_tag="VFF22_02615"
     CDS             3030..3533
                     /locus_tag="VFF22_02615"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009396150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HZX15746.1"
                     /translation="MRKLIIEPLSKEAFAPFGDVIETEGSEFFMINNGSTRRYHKLAT
                     VETAQPEDKAIISIFSAEALDMPLTIRMLERHPQGSQAFIPLLGNPFLIVVAPVGDAP
                     ESELVRAFRSNGRQGINYHRGVWHHPVLTIEKRDDFLVVDRSGSGNNCDEHFFTDDEQ
                     LLLAPHQ"
     gene            3581..>4217
                     /locus_tag="VFF22_02620"
     CDS             3581..>4217
                     /locus_tag="VFF22_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HZX15747.1"
                     /translation="MEAHFTEWLNLGIRWIHMITGIAWIGASFYFVWLENNLNRSNPR
                     EGLSGDLWAIHGGGIYHLEKYKLAPPKMPENLHWFKWEAYATWLSGVALMLVVYYLNP
                     SLYLVKPGVDLAPEMAIVIGFGSMIAGYIAYHFLCDSPLGKRPALLGAVLFVLIIAAA
                     YGFSQIFSGRAAYIHVGAIIGTIMVGNVFFTIMPAQRALVKAIEEHVADHDG"
BASE COUNT          943 a         1320 c         1146 g          808 t
ORIGIN      
        1 tcgctgcctg ggcaaccatg ggcggcatcc agtacgtgtg tggtcaatcg tcccatcgct
       61 tatgggcacc tgcgcagata agcctacgta gacgccgcac ctcctctact tttattgaat
      121 tcggcagatt tatcaggcta agcagcctcg actgagctga agcatagcat gctgaaattc
      181 aaaaataaga cacttttata aaaaactgta cacaataaat cgatcattat gcctgctcag
      241 cgcaccgtaa acgggcagta aatcggccat aagtgcttgc gagcactgct tttgcacagc
      301 aacagctgac gcactggaca gaatcagcat taaggcaaaa tttcgcaaaa taagtgaatt
      361 ttagatttac aagatgcaaa gcactttgta tacaattagc ccatcacggt cgtgcaacgc
      421 attcatgcct gcccaatgac cgccataaac agcaagaagg aagactgcag tgagcgctga
      481 ctacccacgc gacctgatcg gttacgccaa caacccgccc catccacact ggcccaatga
      541 tgcgcgcatc gccctgtcgt ttgttctcaa ttacgaggaa ggcggcgagc gcaacatcct
      601 gcacggcgac aaggaatctg aggcgtttct ctccgagatg gtttccgcgc agccgctgca
      661 aggccagcgc aacctgtgca tggaatcgct ctacgagtat ggcagccgtg ccggcgtctg
      721 gcgtttgctg aacctattcc agaaacacaa catcccgctg accatctttg ccgtggccat
      781 ggccgcgcag cgtcatccag atgcgatcaa ggcgatggtc gccgcaggcc acgagatctg
      841 cagccacggt taccgctgga tcgactacca gaacatgagc gaagccgaag agcgcgagca
      901 tatgctcgaa gccatccgca tcctcaccga actgactggc cagcgcccgc agggttggta
      961 caccggtcgt accgggccga acactcgccg cctggtgcgc gaggaaggcg gcttcctgta
     1021 tgacagcgac acctacgacg acgacctgcc ctactgggac ccggccagca ccgcagccaa
     1081 accgcatctg gtgattcctt acacgctgga caccaacgac atgcgcttca cccaggtgca
     1141 gggcttcaac aagggtgacg atttcttcga atacctgaaa gacgccttcg atgtgctcta
     1201 cgccgaaggc gtggccggcg cgccgaagat gctgtcgatt ggcatgcact gccgcctgct
     1261 tggccgccct gcacgcctgg cctcgctggc gaagtttctt gaatacgtgc agagccacga
     1321 aaaaatctgg tgcgcccgcc gcgtcgacat cgcccaacac tggtacgcaa cccatccgtt
     1381 gcacgatcag ccctccaagg agagctcgaa atgagccgtt ttcaaacgct gactccatcc
     1441 cgcttgaacc gtgaagactt cgtcaaagtc ttcgccgata tttacgagca ctcgccctgg
     1501 gtggccgaga aggcttttga cctggaccag gacgacagca tcaataacat cgacggcctg
     1561 catcagcgca tggccgacct gttgctcagt gccagccatg ctgcacagct ggaattgatc
     1621 aatgcccacc cggacctggc cggcaaggca gccgtacgcg gtgaactgac cgcctccagc
     1681 accgccgaac aagccggcgc cggtatcagc gaatgcaccg ccgacgaatt cgcccgcttt
     1741 accgaactca acgatgccta caaggctaag ttcggcttcc ctttcatcat ggcggtgaaa
     1801 ggcagcaatc ggcaccagat cctggcggcc ttcgaggagc ggattcataa ctccccggag
     1861 caggaattcg cctgcgccct ggccgagatc aacaagatcg cgctgttccg tttgcagcag
     1921 ctataggccg caagccctcc gcctcccaac gccatcatcc aaggcagaca cttcatgaaa
     1981 gcttacgccg taccctttga gaaatacgtc aacctcgccg acgcacgcct gggcacccag
     2041 gccatctcgg tcaccgacga ctggttcgcc gacgttaacc gtctgttcca gccgacccca
     2101 gccgtatgga aggaaggcgt gttcgatgac aacggcaagt ggatggatgg ctgggaatcg
     2161 cgccgcaagc gcttcgaggg ttacgacagt gcagtgatcc gcctcggcgt acccggctcg
     2221 atcaagggcg tggacatcga caccagcttc ttcaccggca atttcccgcc gtcggcgtca
     2281 ctggaaggct gcttcgtcgc tgaaggcgac ccaaccgacg ccaccgagtg gaccgaagtg
     2341 ctggccgcag tagagctaca gggcaacagc caccactacc atgaaatcgc cactgctcag
     2401 gccttcaccc acctgcgctt caacatctac ccggacggcg gcgtcgctcg tctgcgtgtg
     2461 tacggcgtgc agttccgcga ctggagcaat gtcggcgaca acgagcagat cgacctggct
     2521 gccgcgctga atggcggtcg cgccctggcc tgctcggacg aacacttcgg ccgcatgagc
     2581 aacatcctca acccgggccg cggcatcaac atgggcgacg gctgggaaac cgcacgtcgc
     2641 cgtaccccag gtaacgactg ggtgattgtc gccctcggcc atccgggcga aatcgagcga
     2701 atcgtcgtcg acaccctgca cttcaaaggc aactacccgg acagctgctc gatccaggcg
     2761 gccttcgtca aaggcggtac cgacagccag atcgaaaccc agagcctgtt ctggcgcgaa
     2821 ctgctgccaa gccagaagct gcagatgcac gccgagcacg agttcagcga gcagatcaag
     2881 gcgctgggcc cgatcaccca tgtacgggtg aacgtgttcc cggacggtgg tatcagccgc
     2941 ctgcgcctgt tcggcaaagt cgccaagtaa gccaataaac ccctctccca ttgggagagg
     3001 ggcataaccg aacgaacaag aagaccaaca tgcgcaaatt gatcatcgag ccgctgagca
     3061 aagaagcctt cgcccccttt ggtgacgtta tcgaaaccga aggcagcgaa ttcttcatga
     3121 tcaacaatgg ctccactcgc cgttatcaca aactggccac ggtcgagacc gcacagccag
     3181 aggataaggc gatcatcagc attttcagtg ccgaggcgct ggatatgccg ctgaccattc
     3241 gcatgctgga gcgtcatccg cagggcagtc aggcatttat tccgctgctc ggcaacccct
     3301 ttctgattgt ggtcgcgcca gttggcgatg cacctgaatc ggagttggtc cgcgccttcc
     3361 gcagcaatgg caggcagggc attaattacc atcgcggcgt ttggcaccac ccggtgctga
     3421 cgatcgaaaa gcgggatgac ttcctggtgg ttgatcgcag tggttctggc aacaactgcg
     3481 atgagcattt ctttaccgac gacgagcaac tgctcctcgc cccccaccaa taagagaagc
     3541 ccagcaccgg cgtgccggca ctgggaaaga ggtactgatt gtggaagcgc attttactga
     3601 gtggctgaac ctgggtatcc gctggatcca tatgatcacc ggcattgcct ggatcggcgc
     3661 atccttctat ttcgtctggc tggaaaacaa cctcaaccgc agcaatcccc gcgaaggtct
     3721 gtcgggtgac ctctgggcga ttcacggtgg cggcatctac cacctggaaa aatacaaact
     3781 ggccccgccg aaaatgccgg agaacctgca ttggttcaaa tgggaggctt acgccacctg
     3841 gctgtcgggc gtggcgctga tgctggtggt ctactacctc aacccgagtc tgtacctggt
     3901 caagccgggt gttgatctgg caccggaaat ggccattgtc atcggctttg gttcgatgat
     3961 tgccggttac atcgcctatc acttcctctg cgattcgccg ctgggcaagc gcccggccct
     4021 gcttggcgcc gtgctgttcg tactgatcat cgctgccgct tatggcttca gccagatctt
     4081 cagcggccgc gctgcctata tccatgtcgg cgccattatc ggcaccatca tggtcggcaa
     4141 cgtgttcttc accatcatgc cggcccagcg cgcgctggtt aaggccatcg aagaacacgt
     4201 tgccgaccat gatggtg
//
LOCUS       DASRUM010000034        21307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1022131, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000034 DASRUM010000000
VERSION     DASRUM010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 21307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21307
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022131"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VFF22_02625"
     CDS             complement(<1..455)
                     /locus_tag="VFF22_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15748.1"
                     /translation="MSKAFVYTELQIALPFEKAPWRELNPELLRQPGLNNKTWLSGVA
                     NLSLGGFYEFDSIDNAKKFVTGYFPAEARKFGVAQTTRVFAMDVVEEASRQLNSVHFG
                     GIASRTPGAFVYTEVQVSVPFDQAPWRTMNPILRAQPGLLHKTWLSGVD"
     gene            599..1198
                     /locus_tag="VFF22_02630"
     CDS             599..1198
                     /locus_tag="VFF22_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014336678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZX15749.1"
                     /translation="MSRSEQKLQTRQRILEAAGRGFRQGGFGGIGVDGLAKAAGVTSG
                     AFYTHFDSKAAAFRESVAQGMSDLKEGIRHFQTSYGPTWWAEFVKFYLGAKRKCELPE
                     SCALQSLAPEVTRSDATARAEFETELLAVAALIASGPAATDAPVTIGAAMAALSTLAG
                     AVTLARAVCDTAVAEQIVSATEHALLPTQAIAHLVTKGE"
     gene            1294..1545
                     /locus_tag="VFF22_02635"
     CDS             1294..1545
                     /locus_tag="VFF22_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017847680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1654 domain-containing protein"
                     /protein_id="HZX15750.1"
                     /translation="MAATKSAAVKLPSPYELLAQRVQKVINSPNAQKAKAALISKHPD
                     DSDDDWAQLLGEIAENDNVTLAHRDDGAVQLFWQPPCED"
     gene            1640..2728
                     /locus_tag="VFF22_02640"
     CDS             1640..2728
                     /locus_tag="VFF22_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HZX15751.1"
                     /translation="MSLMTIVYLIAGLVLLVAGAEVLVRGAAKLAAQFGIPPLIIGLT
                     VVAFGTSAPETAVSVQAALNGSGDLAIGNVIGSNIANVLLILGLTAVVAPLIVSRQLI
                     RLDVPIMIGASLVVYALAWDGSLSRLDGALLFAGVLAYTSFLIISSRRDKTASEDDEF
                     AKEFGLNEPAKPYAWAINLGLITAGLVLLVSGSNFLVESAVTMARALGISELVIGLTV
                     VAIGTSLPELATSIMAAIKGERDIAVGNIVGSNIFNLLCVLGLASMVSPQAISVAANA
                     LAFDFPVMIAVAVACLPIFFAGYRINRWEGLLFLAYYVAYTVYLVLSSTGRSFAEVFG
                     DAMLGYALPLTAVTLVIIAGRAWHKQKV"
     gene            2739..3116
                     /gene="pcaC"
                     /locus_tag="VFF22_02645"
     CDS             2739..3116
                     /gene="pcaC"
                     /locus_tag="VFF22_02645"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HZX15752.1"
                     /translation="MSDSHEQGLKVRREVMGDAFVDRALGNATDFTQPLQDFVNEHAW
                     GGVWTRGGLDRKTRSLITLSALTALKCPQELKGHVRGALNNGCTVEEIRETLLHCAVY
                     AGVPAAIDAFRAAQEVIDDYQHV"
     gene            complement(3172..4113)
                     /locus_tag="VFF22_02650"
     CDS             complement(3172..4113)
                     /locus_tag="VFF22_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HZX15753.1"
                     /translation="MLAVVQQTLAITAPVFAMLFLGVVLKRLHWIDTAFINTASSLVF
                     RGTMPTLLFLGIVKADLSAALQPALLLFFVVATLVCFALAWCWAIWRIPHDDRGIYTQ
                     GAFRGNNGIIGLALATSLYGDYGLSLGAVLGGVVILCYNVLSAIVLAIYSPGAKTGPL
                     AICKSILINPLIIGVLLAIPFAYWRIPLPAWLMTSAEYFAQMTLPLALICIGGTLSLA
                     SLRQSSGTALSASLMKMVWLPLLATLGAWLYGFREADLAILFLYFASPTAAASFVMAR
                     AVGANHELAAAIIVITTLVAVVTTNLGLLLLQWGGWI"
     gene            complement(4202..4762)
                     /locus_tag="VFF22_02655"
     CDS             complement(4202..4762)
                     /locus_tag="VFF22_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZX15754.1"
                     /translation="MSDEVLFEGEEQPHLLLVDDDPTFTRVMARAMSRRGLRVSTAGS
                     AEEGLALAEQDVPDYAVVDLKMEGDSGLVLLPKLLELDAEMRVVILTGYSSIATAVEA
                     IKRGACNYLCKPADADDVLAALLTQHADLDTLVPENPMSVDRLQWEHIQRVLSEHEGN
                     ISATARALGMHRRTLQRKLQKRPVRR"
     gene            complement(4775..6028)
                     /locus_tag="VFF22_02660"
     CDS             complement(4775..6028)
                     /locus_tag="VFF22_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX15755.1"
                     /translation="MLAPVQLLSASRQNLWRLTLIRILVLAAQAGSVGLAYKSGALPL
                     PWLELSITLGISLVLCLLTALRLRGPWPVTEQEYAVQLGCDLIIHSLLLYYSGGSTNP
                     FVSYYLVPLTIAAATLPWLYTITLSGLALGGYTFLLVWSHPLELPQGPRESLLIYGMW
                     LSFALAAGLITFFVAKMAEELRHQEELRAERREEGLRDQQLLAVATQAAGAAHELGTP
                     LATMSVLIKELRREHQEPTLQEDLAVLQEQVKLCKETLQHLVRAAEADRRQAVIELSS
                     VEWLETTLNRWHLMRPEASYRYQCLGRGAPPRMMPPADLTQALLNLLNNAADACPDKL
                     DIRLDWDHQWLRLSIRDFGAGVPLAIAEQLGRPFFTTKGKGFGLGLFLSQASVTRAGG
                     TVKLYNHEEGGTLTELKLPRAYGRS"
     gene            6239..6952
                     /locus_tag="VFF22_02665"
     CDS             6239..6952
                     /locus_tag="VFF22_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242844.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HZX15756.1"
                     /translation="MLPLSRLATALLLSTATLLSATAMAEEARYNQVALRAEVTQEIA
                     HDLMHVTLYSEAQDSDPAALAAQISKTLNASIAQARKVKGVSVASGSRNSYPVYDDKG
                     QKITAWRERAELRLESADFSALSKLTGEMLQTLKMGGMNFSIASDTRKTHEDALLKDA
                     VAAFKARAQLATEALGGSGYKLVSLSLNSGGFQPPMPMRMEAMKGAAMMDAAVTPEIE
                     AGTSQVNINADGVIEVQMP"
     gene            7176..8774
                     /locus_tag="VFF22_02670"
     CDS             7176..8774
                     /locus_tag="VFF22_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX15757.1"
                     /translation="MRKNAVIQALLAAGLIASTPLASAAGNLVFCSEGSPAGFDPGLY
                     TTGTDFDAAAETVFNRLSQFERGGTKVIPGLAEKWDISEDGLSYTFHLRPKVKFHTTE
                     YFTPSRDFNADDVLFTFQRMLDKDHPFRKAYPSEFPYFTDMGMDANIAKIEKLDDMTV
                     KFSLNEVDAAFIQNLAMSFASIQSAEYADSLLKAGKAADINQKPIGTGPFVFSRYQKD
                     AVIRFKGNKEYWQPDEVKIDNLIFAINTDASVRMQKLKAGECQVTLFPRPADLDALKK
                     DPNLDMPEQAGFNVGYIAYNVTHPPFDQLEVRQALDMAVNKQGIIDAVYQSAGQLATN
                     GMPPTQWSYDESIKDPAYNPEKAKELLKAAGIKEGTEITLWAMPVQRPYNPNAKLMAE
                     MLQSDWAKVGIKAKIVSYEWGEYLKRAKAGEHDAMLIGWSGDNGDPDNWLGTLYGCDA
                     VDGNNFSKWCHADYDKLVKAAKRVTDTAERTELYKQAQVILKREVPITPIAHSTVYQP
                     MSKKVEDFRISPFALNSFYGVGVK"
     gene            complement(8834..10060)
                     /locus_tag="VFF22_02675"
     CDS             complement(8834..10060)
                     /locus_tag="VFF22_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZX15758.1"
                     /translation="MTLGVGGCTPQQALAGLHNMMDGAQPIALGEYQARVSRAQARMQ
                     TEGIAAMFINAGSNLQYFTGVRWHPSERMVGAILPADGSLEYLAPAFEVGTIRDFQVL
                     AGAINTWQEHESPYQLLLDTLQRMGITPDPEQPVKIGLCPTLAFFMVDGIRRLARGYE
                     FVDGAVISAYCRQRKSAAELALMQRAKDMTLEVHKAVASVLHEGISTTAVAEFIQQAH
                     RAVGAAGSTFCIVLFGPASSFPHGVKHAQTLKQGDMVLIDTGCQVHGYLSDITRSYVF
                     GDPSQRQRDFWNIEKAAQLAAFEAAQLGQPCQAVDAAARRTLEAAGLGPDYKLPGLPH
                     RTGHGIGLDIHEGPYLVRGDLTPLAEGMCFSNEPMICVPGEFGVRLEDHFYMSADGPR
                     WFTAPSHSLDDPFGLG"
     gene            10229..10792
                     /locus_tag="VFF22_02680"
     CDS             10229..10792
                     /locus_tag="VFF22_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZX15759.1"
                     /translation="MTADRIGERLRRYRRAAKKTLSEVASESGLTASFLSQAERNLTG
                     ISISSLASIAKSLDVPLNVLFDQPPQPEPDSHQGERVRYTIEGQPLAYERLSSSFPGN
                     QLNAVKMSMPVGYQSELIAHEGAEFAYVLSGKILYTIDGRQYPLSAGDSVHFDAGKAH
                     RLANVGKDVAEMLAITTLSLFDDHSTP"
     gene            11060..12631
                     /locus_tag="VFF22_02685"
     CDS             11060..12631
                     /locus_tag="VFF22_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX15760.1"
                     /translation="MLVAGLFLGSTYASASNLVYCSEGSPGGFDPGQYTTGTDFDAAS
                     ETLFNRLAQFERGSTLAVPALATEWQASEDGLSYTFKLRPGVKFHSTEYFTPSRTFNA
                     DDVVFTFQRMLDKNHPFRKAYPTEFPYFTDMGLDKNIAKVEKVDDLTVRFTLNSVDAA
                     FIQNLAMNFAAIHSAEYADHLLKTGKAADINQKPIGTGPFVFNRYQKDAQIRFKGNKD
                     YWLPEDVQIDNLIFAINTDASVRAQKLKAGECQITLNPRPADLAALQKDPNLQVPSQP
                     GFNLGYIAYNVTRAPFDKVEVRQALDMAIDKPAIISAVYQGAGQLAVNAMPPTQWSYD
                     ESINAIPQDIEKAKALLKQAGVAEGTKITLWAMPVQRPYNPNAKLMAEMLQADWAKVG
                     IKAEIVSYEWGEYIKRAHAGEHDAMLIGWTGDNGDPDNWLGTLYGCDSVNGNNFSKWC
                     DAGYDKIVKQAKAITDRDARSELYKQAQQIIQQQLPISPIAHSTVYQPMRNSVKGFEI
                     SPFGRNSFYGVSNTR"
     gene            12846..13028
                     /locus_tag="VFF22_02690"
     CDS             12846..13028
                     /locus_tag="VFF22_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15761.1"
                     /translation="MTFALRASIVLSLALGCAAGQAKPLVVCTEAPPVSAVSRDVEGF
                     TLSPMGSNNYAKVKRH"
     gene            13159..14169
                     /locus_tag="VFF22_02695"
     CDS             13159..14169
                     /locus_tag="VFF22_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZX15762.1"
                     /translation="MFAFIARRLGLLIPTFFGVTLLTFALIRMIPGDPVEVMMGERRV
                     DPEMHAQAMERLGLNKPLYAQYFDYIGKLAQGDLGESLRTRTSVWDEFTTLFPATLEL
                     SLAALLFAGTLGLIAGVIAALKRGSLFDHGVMGISLAGYSMPIFWWGLLLIMFFSVWL
                     GWTPVSGRIDLLYDIPPVTGFMLIDTLLSDEEGAFVDALRHLILPAIVLGTIPLAVIA
                     RMTRSAMLEVLREDYVRTARAKGLSPARVVFVHGLRNALIPVLTVFGLQVGALLAGAV
                     LTETIFSWPGIGKWLIESIGARDYPVVQNGILLIACLVILVNFVVDILYGLANPRIRH
                     QR"
     gene            14180..15082
                     /locus_tag="VFF22_02700"
     CDS             14180..15082
                     /locus_tag="VFF22_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZX15763.1"
                     /translation="MTTQATLDQSLFYPSPLKEFWQAFAHNKGAVAGLLFMIVLVICA
                     LFAPWIAPHDPSEQYREFLLTPPAWLEGGQLQFLLGTDEVGRDLLSRLIHGARLSLLI
                     GLASVLMSLIPGILLGLVAGFFPRLLGPSIMRLMDIMLALPSLLLAVAIVAILGPGLI
                     NTIFAIAIVSLPSYVRLTRAAVMGELNRDYVTAARLAGASLPRLMFITVLPNCMAPLI
                     VQATLSFSSAILDAAALGFLGLGVQPPTPEWGTMLASARDYIERAWWVVSLPGLTILL
                     SVLAINLMGDGLRDALDPKLKNAQ"
     gene            15093..16067
                     /locus_tag="VFF22_02705"
     CDS             15093..16067
                     /locus_tag="VFF22_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX15764.1"
                     /translation="MSLLDIKNLSVRFGDATAIPVVDGLDLCVEKGEVLAIVGESGSG
                     KSVTMMALMGLIDAPGIVTADSLQFDGTDMLRLKGRQRRHIVGKDLSMVFQDPMTALN
                     PSFTVGFQIEEVLRQHLNLKGQAARQRALQLLDRVEIPAAASRLNAYPHQLSGGMSQR
                     VAIAMAIAAEPKLLIADEPTTALDVTIQAQIMDLLVGLQREQNMGLVLITHDLAVVAE
                     TAQRVCVMYAGQAVEIGNVPQLFTAPSHPYTEALLKAIPEHSQGQRRLTTLPGIVPGR
                     YDRPVGCLLSPRCPYAQAHCREQRPTLDAHERGAARCFYPLNLTAEVA"
     gene            16067..17038
                     /locus_tag="VFF22_02710"
     CDS             16067..17038
                     /locus_tag="VFF22_02710"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010565544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HZX15765.1"
                     /translation="MSAVLTARDLTRHYEISRGLFQPSAQVQALNGVSFELQAGKTLA
                     VVGESGCGKSTLARALTLIEEPSSGSLQIAGQEVTGANKAQRKQLRRDVQMVFQNPYA
                     SLNPRQKVGDQLGEPLLINTGLSRLERRERVQTMMQQVGLRPEHYQRYPHMFSGGQRQ
                     RIALARAMMLQPKVLVADEPTSALDVSIQAQVLNLFMDLQEQFNTGYVFISHNLSVVR
                     HVADDVLVMYLGRPVEMGPSELIYSRPLHPYTQALLSATPTIHPDPSKPKIKISGELP
                     NPLSPPSGCAFHQRCPHATDRCQVEQPALRLLDARQVACHHAEQINS"
     gene            complement(17069..17845)
                     /locus_tag="VFF22_02715"
     CDS             complement(17069..17845)
                     /locus_tag="VFF22_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HZX15766.1"
                     /translation="MPIRYILKQFFLPPGLFLLLLLAAWWLRRRFPRLALGCFVAGLG
                     GMWLFSLPVVVEYSARILETEPALNERQWTDLARQAEVIVVLGGGRELADPGWGADQP
                     SLMALERVRFAARLAKASGLPLLTSGGLHYGVPPSEAALMAEVLARDYDVPVRWQEGL
                     SRTTWENSTHSAELLKQAGITRVVLVTQAWHMPRSRWSFEQAGLQVVSAPMGFLGTPN
                     GRPAGGWLPESKAVWQNGLLLNEAIGLLGYRLFYPGTAAP"
     gene            complement(17872..18315)
                     /locus_tag="VFF22_02720"
     CDS             complement(17872..18315)
                     /locus_tag="VFF22_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15767.1"
                     /translation="MAEFRRGAAGGGLYERLLQRLAMALDEADNLSVLSAQPQQELEL
                     RGLTAAEMELIRAYLDRDLGWLRGWHAAAQEMAQIEQLPSSRKVGRSGHKPTAALPGK
                     IKPLLKRRLQLCCALCGTQAGWQSGNGVQACKACGSQLFRVTNHR"
     gene            18560..21172
                     /gene="leuS"
                     /locus_tag="VFF22_02725"
     CDS             18560..21172
                     /gene="leuS"
                     /locus_tag="VFF22_02725"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZX15768.1"
                     /translation="MQEQYSPREIEAAAQSHWDAQKSFVVSEQPGKESFYCLSMFPYP
                     SGKLHMGHVRNYTIGDVIARYQRMLGKNVLQPMGWDAFGMPAENAAMKNQVAPAKWTY
                     ENIEYMKTQLKSLGLAVDWTREVTTCKPDYYRWEQWLFTRLFEKGVIYRKNGSVNWDP
                     VDQTVLANEQVIDGRGWRSGAVIEKREIPMYYFKITAYAEELLSSLDELDGWPEQVKT
                     MQRNWIGKSFGADVVFDYDQASVGIDGQLKVYTTRPDTLMGATYVAVAAEHPLAQRAA
                     ESNPAIAAFIAECKAGSVAEADMATMEKKGVATGQFVIHPLSGDKLPVFVANYVLWGY
                     GEGAVMAVPAHDERDFEFANKYDLSIKQVYAGEGKEYDATQWQDWYGDKAGLSTIGSG
                     KYDNLDFTAAFDAIVADLEANAHGARKTQFRLRDWGISRQRYWGCPIPIIHCDTCGDV
                     PVPEDQLPVVLPEDVVPDGAGSPLARMPEFYECSCPKCGAPAKRETDTMDTFVESSWY
                     YARYASPHYTGGMVDPEAANHWLPVDQYIGGIEHAILHLLYARFFHKLMRDEGLVSSN
                     EPFKNLLTQGMVVAETYYRTLENGGKDWFNPADVELERDSKAKVISAKLKSDGLPVEI
                     GGTEKMSKSKNNGVDPQSMIDQFGADTCRLFMMFASPPDMSCEWSDSGVEGANRFLRR
                     VWRLAQNHVNQGIVGALDLASLDDEQKAVRRAIHLAIKQSSFDIGQHHKFNTAIAQVM
                     TLMNVLEKAAQTTAQDCALMQEGLEAVALLLAPITPHISHQLWQELGHDEAIIDASWP
                     QVDESALVQDSLTLVIQVNGKLRGQIEVPASASREDVEATARSNENVLRFTEGLSIRK
                     VIVVPGKLVNIVAN"
BASE COUNT         4461 a         6975 c         6139 g         3732 t
ORIGIN      
        1 ttatccacac cactcaacca ggttttgtgc agcaacccag gctgcgcccg aagaattggg
       61 ttcatggtgc gccaaggcgc ctgatcaaac gggaccgaaa cctgcacttc ggtgtagacg
      121 aatgcacccg gcgtgcgact ggcgatacca ccgaagtgca ccgaattcag ctgtcggctg
      181 gcctcctcga ccacgtccat ggcaaacacc cgagtcgttt gcgccacgcc aaatttgcgg
      241 gcttctgccg ggaagtagcc ggtgacgaat tttttcgcgt tatcgattga gtcgaactca
      301 tagaaacctc ccagtgacag gttcgccacc ccagacaacc aggttttatt gttcaacccc
      361 ggctgcctga gtaactcagg gttgagctcg cgccacggcg ccttctcgaa tggcaaagcg
      421 atttgcaact cggtgtagac aaaagctttc gacatagcgg catccctata gattggctaa
      481 tccagtcata gccatgacca gaacgaccgc caagataacg atcgttattt taaaatacat
      541 aacgatcgtt acccgaacaa gtataatttc cactcagcac ccaacgaagg agcgattcgt
      601 gtcgcgatcc gaacagaagc tacaaactcg ccagcgcatc ctcgaagcgg caggtcgcgg
      661 ctttcgccaa ggcggttttg gtggcatagg tgtcgatggc ttggcaaaag cggccggggt
      721 cacgtccggc gccttctata cgcacttcga ctctaaggcc gccgcgttcc gcgagtccgt
      781 tgcacagggt atgtcggacc tcaaggaagg catacgccat tttcaaacca gctacgggcc
      841 gacctggtgg gctgagttcg tgaagtttta cctgggtgcc aagcgcaagt gtgagctccc
      901 cgaaagttgc gccttacaaa gcctcgcccc cgaagtgacg cgctccgatg cgacagcccg
      961 ggcggagttc gaaactgaat tgcttgcggt cgcagccctg atcgcaagcg ggcccgcagc
     1021 cacggacgcc cccgtcacta tcggtgcagc aatggccgca ctgagcacac tagccggcgc
     1081 ggtcaccctc gcccgagcgg tttgcgatac ggcagtggcc gagcaaatag tcagcgcaac
     1141 agagcacgcg ctgttgccaa ctcaggccat agcgcacctg gtgactaagg gtgaatgatt
     1201 gacgcaccgc ccccccttcg tggcttgacc tgacatcggt acgcctgaca acattctgta
     1261 tgcataaaca gttatatttc aggagccctg cgtatggccg ccactaaatc tgccgctgta
     1321 aaactaccca gtccctacga actgctggct caacgggtgc agaaagtcat caactcgcca
     1381 aacgcccaga aggccaaagc cgcgctgatc agcaagcacc ccgatgactc ggatgacgac
     1441 tgggcacagc tcttgggcga aatcgccgag aacgacaacg tgaccctggc ccaccgcgac
     1501 gatggcgccg tgcagttgtt ctggcagccg ccctgcgagg actgacgcag gcgcaacatc
     1561 aggcaacaat gcgcactcac aggagctggc gagcagttac agattgggta agctaggcgg
     1621 acttttttgg agcgcaatca tgtccttaat gaccatcgtc tacctgatcg ccggcttggt
     1681 gctgctggtt gccggcgccg aagtactggt acgcggagcc gccaaactgg ccgcgcaatt
     1741 cggcattcca ccgctgatca tcggcctgac cgtggtggca ttcggcacca gcgcgccgga
     1801 aaccgccgtc agcgtgcaag cggcattgaa tggcagcggc gacctggcga ttggcaacgt
     1861 aatcggcagc aatatcgcca acgtactgct gattctcggc ctgaccgctg tggttgcacc
     1921 gctgatcgtc tcgcgccagc tgatccgcct ggatgtgccg atcatgatcg gcgccagcct
     1981 ggtggtctac gccctggcct gggatggcag cctcagccgc ctcgacggcg cgctgctgtt
     2041 tgccggcgta cttgcctaca ccagcttcct gattatcagc tcgcgccgcg acaaaaccgc
     2101 cagcgaggac gacgaattcg ccaaggagtt cggcctcaat gaaccggcca agccttacgc
     2161 ctgggctatc aatctggggc tgatcactgc cggcctggtg ctgctggtca gcggctccaa
     2221 cttcctggtt gagagcgccg tcaccatggc ccgcgcactg ggcatctcgg aactggtgat
     2281 cggcctgacc gtggtcgcca tcggtacctc gctgccggaa ctggccacct cgatcatggc
     2341 cgccatcaag ggcgagcgcg atatcgccgt gggcaatatc gtcggcagca atatcttcaa
     2401 cctgctctgc gtactgggcc tggcctcgat ggtctcgccg caagccatca gcgtggccgc
     2461 caacgccctg gccttcgact tcccggtgat gatcgccgtg gccgtggcct gcctgccaat
     2521 tttcttcgcc ggctaccgca tcaaccgctg ggaaggcctg ctgttcctcg cctactacgt
     2581 ggcctacacg gtctacctgg tgttgtcgtc gaccggccgg tcgttcgccg aagtcttcgg
     2641 tgacgccatg ctcggctacg ccctgccgct gaccgcagtg actctggtga ttattgccgg
     2701 gcgggcctgg cataaacaga aggtttgagg agagcgaaat gagcgatagc cacgagcaag
     2761 gcctgaaagt ccgccgcgaa gtgatgggcg acgctttcgt cgaccgcgcc ctgggtaacg
     2821 ccaccgactt tacccagccg ctgcaggact tcgtcaacga acacgcctgg ggcggcgtgt
     2881 ggacccgggg cgggctggat cgcaagaccc gcagcctgat cacgctgtcg gcgcttactg
     2941 cactgaaatg cccgcaagag cttaagggcc acgtgcgcgg cgcgctgaac aacggctgta
     3001 cggttgagga aatccgcgaa accctgctgc attgcgcggt gtatgctggc gtgccggcgg
     3061 ccatcgatgc cttccgcgcg gcgcaggaag tgatcgacga ctaccagcat gtctgagcgc
     3121 aacacaacaa aaagccgggc actgcccggc tttttaatgc gcgccgcata gctaaatcca
     3181 gccgccccac tgcaacagca atagcccaag gttggtggta accaccgcta ccaaggtggt
     3241 gatcacgatg atcgccgccg ccagctcatg attggcgccc accgcgcggg ccatgacgaa
     3301 gctggccgcc gccgtggggc tggcgaaata cagaaagagg atcgccagat ccgcctcgcg
     3361 aaagccatac agccaggcac ccaaggtggc cagcagcggc agccagacca tcttcatcag
     3421 gctcgcactc agcgccgtgc cgctgctctg gcgcagcgag gccaggctaa gggtgccgcc
     3481 aatacagatc agcgccagcg gcagggtcat ctgtgcgaag tactcggccg aggtcattag
     3541 ccaggccggt aatggaatgc gccagtaagc gaagggaatc gccagtagca cgccgatgat
     3601 cagcggattg atcaggatgc tcttacagat ggccaaaggc ccggtcttgg cgcccgggct
     3661 gtagatcgcc aggacaatgg ccgagagcac gttgtagcaa aggatcacca ccccgccgag
     3721 caccgccccc agggacaggc cgtagtcgcc gtacagactg gtggccaacg ccagaccgat
     3781 gatgccgtta tttccgcgaa acgcaccctg ggtgtagatg ccacgatcat catgagggat
     3841 gcgccagatc gcccagcacc aggccagggc aaagcaaacc agggtcgcga caacgaaaaa
     3901 cagcagcagc gccggctgca gcgccgcgct cagatcggct ttgacgatgc cgagaaacag
     3961 cagggtcggc atggtgccgc gaaacaccag gctcgaggcc gtgttgataa aagcggtgtc
     4021 gatccagtgc aggcgtttga gcaccacgcc gagaaacagc atggcgaata ccggtgcagt
     4081 gatggcaagg gtttgctgga cgacggcaag catggaagca ccagaggtgt tgtctgacaa
     4141 cctatgttag ctggctaacc attgccttgc taccgtctcc tacatcagca cgcagccctc
     4201 ttcagcggcg taccgggcgc ttctgcagtt tgcgctgcaa ggtgcgccga tgcatgccca
     4261 gagcgcgggc ggtggcggag atattgcctt catgctcgct gagcacgcgc tggatgtgtt
     4321 cccactgcag gcggtccacc gacatcgggt tttccggaac cagggtatcc aggtcggcat
     4381 gctgagtcag cagtgcggcc aacacatcat cggcatcagc cggcttgcac aggtagttgc
     4441 aggcgccacg cttgatcgcc tccaccgctg tggcaatgct cgaatagccg gtgagaatca
     4501 ccacgcgcat ttcggcgtcc agctccagca gcttgggcag cagcaccaga ccggaatcac
     4561 cttccatctt caagtcgacc acggcgtaat ccggcacatc ctgttcagcc agggccaggc
     4621 cttcttcggc tgagcccgcg gtggaaaccc gcaggccgcg gcggctcatg gcgcgtgcca
     4681 tcacgcgggt aaaggtcgga tcgtcatcga ccagcagcag gtgcggttgt tcttcacctt
     4741 cgaacagcac ttcatcgctc attcgggtag ttccttagct tcggccatag gcgcgcggca
     4801 gtttcagctc ggtgagcgtg ccgccttctt cgtggttata cagtttaacc gtgccgccgg
     4861 cacgggtgac gctggcctgg ctgaggaaca aacccagacc gaaccccttg cccttggtgg
     4921 tgaaaaacgg ccgacccagc tgctcggcaa tcgccagcgg cacacccgcg ccaaagtcgc
     4981 ggatgctcaa gcgtagccac tggtgatccc agtccagacg gatgtccagc ttgtccgggc
     5041 aggcatcggc ggcattgttc agcaggttga gcagcgcctg ggtcaggtcg gccggcggca
     5101 tcatccgtgg cggcgcgcca cggcccaggc attgataacg ataactggct tctggacgca
     5161 tcaggtgcca gcggttgagg gtggtttcca gccattccac cgaggacagc tcgatcaccg
     5221 cctgacgccg atccgcctcg gcggcgcgca ccagatgctg cagagtttcc ttgcacagct
     5281 tgacctgctc ctgcagcacc gccaggtcct cttgcagcgt cggttcctga tgttcgcggc
     5341 gcagttcctt gatcagcaca ctcatggtcg ccagcggcgt gcccaactca tgtgccgcac
     5401 cagcagcctg agtcgccaca gcgagcagct gctggtcacg caagccctct tcacggcgct
     5461 cggcgcgcag ctcttcctga tggcgcagct cttcggccat cttcgccacg aagaaggtga
     5521 tcaagcccgc agccaaagca aagctcaacc acatgccgta aatcagcagg ctttcccgtg
     5581 gcccctgcgg caattccagc gggtgcgacc agaccagcag gaaggtgtaa ccgcccaacg
     5641 ccaggccgga caaagtgatg gtgtagagcc agggcaaggt cgccgccgcg atggtcagcg
     5701 gcaccaggta ataagagaca aaggggttgg tcgagccacc cgagtaatac agcagcaggc
     5761 tatggatgat caggtcgcag cccagctgca ccgcgtactc ctgctcggtc accggccagg
     5821 gcccgcgcag gcgcagggcg gtcagcaggc acagcaccag ggaaattccg agagtgatgc
     5881 tcagttccag ccaaggcagc ggcagcgcgc cagatttgta ggctaggccc acggaaccgg
     5941 cctgcgcggc cagcaccaga atgcggataa gggtcaggcg ccataggttt tgccggctcg
     6001 ccgacagtag ctgcacaggt gcgagcatag aatctccaag ttgctcattc agcgagccgc
     6061 aggagtataa ccaagcctca gtacggccaa ctgcggcaac acgccgcagc cgctggaagc
     6121 ccatccacaa accagagaaa aactccggaa ccggatttaa cccgtttagt ctgatcgcta
     6181 cgtctacccc ctggaaagcc gcgcataacc catgcgccca acatataaag gaatcgtcat
     6241 gctgccattg tcccgcctcg ccaccgccct gctgctgagc accgccaccc tgttgagcgc
     6301 cacggccatg gccgaagaag cccgctacaa ccaggtcgcg ctacgcgccg aggtcactca
     6361 ggaaattgct cacgacctga tgcacgtcac tctctacagc gaagcccagg acagcgaccc
     6421 agccgccctc gccgcgcaga tcagcaaaac cctcaacgcc agcatcgccc aggcgcgcaa
     6481 ggtcaaaggc gtcagcgtcg cctccggcag ccgtaacagc tacccggttt acgatgacaa
     6541 gggccagaaa atcaccgcct ggcgtgagcg cgccgagctg cgcctggaaa gcgccgactt
     6601 cagcgcgctg tccaagctca ccggggaaat gctgcaaacc ctgaagatgg gcgggatgaa
     6661 cttcagcatc gcctcagaca cccgcaagac ccacgaagac gcgctgctca aggacgccgt
     6721 ggcagccttc aaagcccgcg cacaactggc caccgaagcc ctcggcggca gcggttacaa
     6781 gctggttagc ctgagcctga acagcggcgg tttccagccg ccaatgccga tgcgcatgga
     6841 ggccatgaaa ggtgcggcga tgatggatgc ggcggtgacg ccggagatcg aagccggcac
     6901 cagccaggtc aatatcaacg ccgatggtgt gatcgaagtg cagatgccat aaaaactgct
     6961 acaaacagaa gcaacgccgt tatccgacga tagcggcgtt ttgccatatt tttctcgcac
     7021 ttttataaat atgcgacacc aacatactgt tgcgtcgtca ttaccgttct ctctgcgaca
     7081 tgccacttgc ctccggcata cgccctgcat agtttctccc aaggtctcca caagaggccc
     7141 ctcaggatca caaccacaat aaccatgagg tcactatgcg taagaacgcc gtcatccagg
     7201 ctttactcgc cgctgggctg atcgccagca cgccacttgc cagcgccgcc ggcaacctgg
     7261 tgttctgctc cgaaggtagc cccgccgggt tcgaccctgg cctgtacacc accggcaccg
     7321 atttcgatgc agcagcggaa accgtgttca accgtttgag ccagttcgaa cgtggcggca
     7381 ccaaagtgat ccccggcctg gccgagaagt gggacatcag cgaagacggt ctgagctaca
     7441 ccttccacct gcgccccaaa gtgaaattcc acaccaccga atacttcacg ccgagccgcg
     7501 acttcaacgc tgatgacgtg ctgtttacct tccagcgtat gctcgacaag gaccacccgt
     7561 tccgcaaagc ctatccgtcg gagttcccct acttcaccga catgggcatg gacgcaaaca
     7621 tcgccaagat cgaaaaactc gacgacatga ccgtcaagtt cagcctcaac gaagtcgacg
     7681 ccgcctttat ccagaacctg gcgatgagct ttgcttcgat ccagtccgcc gaatacgccg
     7741 atagcctgct caaggccggc aaggccgccg atatcaacca gaagcccatc ggcaccggcc
     7801 catttgtgtt cagccgttac cagaaggacg cggtgatccg cttcaagggc aacaaggaat
     7861 actggcaacc cgatgaggtg aagatcgaca acctgatctt cgccatcaac accgacgcct
     7921 cggtgcgcat gcagaagctc aaggccggcg aatgccaggt caccctgttc ccgcgcccgg
     7981 cggacctgga cgcgctgaaa aaagacccga acctggacat gcccgagcag gccgggttca
     8041 acgtcggcta tatcgcttac aacgtgaccc acccgccgtt cgaccagctc gaagtgcgcc
     8101 aggcactgga catggcggtg aacaagcaag gcatcatcga cgcggtgtac cagagcgccg
     8161 gccagctggc gaccaacggc atgccgccga cccagtggtc gtacgatgaa agcatcaagg
     8221 accccgctta caacccggaa aaagccaagg aactgctcaa ggccgccggt atcaaggaag
     8281 gcaccgaaat caccctgtgg gccatgcctg tgcaacgccc ctataacccc aacgccaagc
     8341 tgatggccga aatgctgcaa agcgactggg ccaaggtcgg catcaaagcc aagatcgtca
     8401 gctacgaatg gggcgaatac ctcaagcgcg ccaaggccgg cgaacacgac gcgatgctga
     8461 tcggctggag cggcgacaac ggtgacccgg acaactggct gggcaccctg tacggctgcg
     8521 acgcggtgga cggcaacaac ttctccaagt ggtgccatgc cgactacgac aagctggtca
     8581 aagccgccaa gcgcgtgacc gacaccgccg agcgcaccga gctgtacaag caggcccagg
     8641 tgattctcaa gcgcgaagtg ccgatcaccc caatcgccca ctccacggtc tatcagccaa
     8701 tgagcaagaa ggtcgaagac ttccgcatca gcccgttcgc gctgaactcg ttctacggcg
     8761 tcggcgtcaa gtaaccaccc tggcaataaa aaacggcggc caatggccgc cgttttattt
     8821 actgcaccag cacttatccc agaccgaatg gatcgtccag cgaatggctc ggcgcggtga
     8881 accagcgcgg cccatcggcg ctcatgtaga agtgatcctc cagacgcacg ccgaactcgc
     8941 ccggcacgca gatcatcggc tcattgctga agcacatgcc ctcggccagc ggcgtcagat
     9001 cgccacgcac cagatacggc ccctcatgga tatccagacc gataccgtgc ccggtgcgat
     9061 gcggcagacc cggcaacttg taatccggac ccaggcccgc cgcttccaaa gtgcgccgcg
     9121 ccgcagcatc caccgcctgg cagggctggc cgagctgcgc agcctcaaat gcggccaact
     9181 gcgcggcctt ctcgatattc caaaagtcgc gttgacgctg actgggatcg ccgaaaacat
     9241 agctgcgagt gatgtccgac aggtaaccgt gcacctggca gccggtgtca atcagcacca
     9301 tgtcgccctg cttgagggtc tgcgcgtgtt tgacgccatg ggggaaggaa ctggccgggc
     9361 caaacagcac gatgcagaac gtcgagcccg ctgcgccgac ggcgcgatgc gcctgctgga
     9421 taaactcggc gaccgctgtg gtgctgatgc cttcgtgcag cacactggcg acggccttgt
     9481 gcacctcaag ggtcatgtcc ttggcgcgct gcatcagggc cagctccgcc gctgatttgc
     9541 gctgccggca gtaagcgctg atcaccgcgc catcgacaaa ctcgtagcct ctggccaggc
     9601 gacgtatgcc gtcgaccata aagaatgcca gcgtcggaca aaggccgatt ttcactggct
     9661 gctctggatc gggcgtgatg cccatccgct gcagggtgtc gagtagcaac tgataagggc
     9721 tttcgtgctc ctgccaggta ttgatggcgc cagcgagcac ctggaaatcg cgaattgtgc
     9781 cgacttcgaa cgccggggcg agatattcca gcgacccatc agccgggaga atggccccca
     9841 ccatgcgttc actgggatgc cagcgcacgc cggtgaaata ctgcagattg ctgccggcgt
     9901 tgataaacat cgcggcaata ccttcggtct gcatccgtgc ctgggcgcga ctcactcgcg
     9961 cctggtattc accaagcgcg attggttgcg cgccatccat catgttgtgt agcccggcaa
    10021 gcgcctgttg cggcgtacaa cctccgacac cgagagtcat ctcgcgctcc tttgtatggc
    10081 tggtgatttg gacgcatgaa atttaagtct gacttaaata gagcgcaacg gttattttag
    10141 tgtcacttaa attcacaggt attgtccgac gacttttctg cacgacgccg aacggtatac
    10201 tggcgctctt tcagcacgag ccagggttat gaccgcagat cgcatcggag aacgtttgcg
    10261 ccgctatcgg cgggccgcga agaagaccct gagtgaagtc gcgagcgagt cggggcttac
    10321 cgccagcttc ctctcccagg ccgagcgcaa ccttaccggc atctcgattt cctcattggc
    10381 cagcatcgcc aagtcactcg acgttccgct taatgtactg ttcgaccaac cgccgcaacc
    10441 cgagcccgac tcccaccagg gcgagcgggt gcgctacacc atcgaaggcc agcccctggc
    10501 ctacgagcgc ctgtccagca gttttccggg caaccagctg aacgcggtga aaatgagcat
    10561 gccggtcggc tatcaatccg aactgatcgc ccatgaaggc gccgaatttg cctacgtgtt
    10621 atccggcaag attctctaca ccatcgacgg ccgccaatat cccttgagtg ccggcgactc
    10681 ggtgcatttc gatgccggca aggcccaccg cctggccaat gtcggcaaag acgtagcgga
    10741 aatgctggcc atcaccaccc tgagcctgtt cgacgaccac agcacgccct gacctcgcgc
    10801 accggtgcta tctagcacag cacggtgaca accaatgtta gtagcgctta atttttgttg
    10861 acctcaattt cagcatgact taatttcgag cgcgacaggt tgctgagcga tttcgctcca
    10921 ccgcagctct gggccggcct cttcacccga caggccggaa cgcagcggtg ctgatcacag
    10981 cccacgaggc tgaccctcaa tgacgcccaa aaaaagaata aatgaggtac tcatgcgcac
    11041 tcacacgtcc atccaggcca tgctcgtcgc cggactgttt ctcggctcga catacgcatc
    11101 cgccagcaac ctcgtgtact gctccgaagg cagccccggc ggcttcgacc cgggccagta
    11161 caccaccggc accgacttcg acgccgcctc ggaaaccctg ttcaaccgcc tcgcccagtt
    11221 cgagcgcggc agcaccctgg ccgtaccggc cctggcgacc gaatggcagg ccagtgaaga
    11281 cggcctcagc tacaccttca agctgcgtcc cggggtgaaa ttccacagca ccgagtactt
    11341 cacccctagc cgcaccttca acgctgatga cgtggttttc accttccagc gcatgctcga
    11401 caagaaccac ccgttccgta aggcctaccc gaccgagttc ccttacttca ccgacatggg
    11461 cctggacaag aacattgcca aggtggagaa ggtcgacgac ctcaccgtgc gtttcaccct
    11521 caatagcgtg gacgcggcct ttatccagaa cctggcgatg aacttcgccg ccatccactc
    11581 cgccgaatac gcagaccacc tgctcaagac cggcaaagcc gccgacatca accagaagcc
    11641 aatcggcacc ggccccttcg tgttcaaccg ctaccagaaa gatgcgcaga ttcgcttcaa
    11701 gggcaacaag gactactggc tgccggaaga tgtgcagatc gacaacctga tcttcgccat
    11761 caacaccgac gcctcggtac gcgcgcagaa gctcaaggcc ggcgaatgcc agatcaccct
    11821 gaacccacgc ccggcggatc tcgccgcgct gcagaaagac cctaacctgc aggtgcccag
    11881 ccagcctggc ttcaacctgg gttacatcgc ctacaacgta acccgcgcgc ccttcgacaa
    11941 agtcgaagtg cgtcaggcgc tggacatggc catcgataaa ccggcgatca tcagcgcggt
    12001 ctaccaaggc gccgggcaac tggcggtcaa cgccatgccg ccgacccagt ggtcctatga
    12061 cgaaagcatc aacgccatcc cgcaagacat cgagaaagcc aaggccctgc tcaagcaggc
    12121 tggcgttgcc gaaggcacca agatcaccct gtgggccatg cccgtacagc gcccgtataa
    12181 ccccaacgcc aaactgatgg ccgaaatgct ccaggccgac tgggccaagg tcggcatcaa
    12241 ggctgagatc gtcagctacg agtggggcga gtacatcaag cgcgcccatg ccggcgagca
    12301 cgacgccatg ctgattggct ggactggcga caatggcgac ccggacaact ggctgggcac
    12361 cctgtacggc tgcgactcgg tcaacggcaa caacttctcc aagtggtgcg atgccggcta
    12421 cgacaagatc gtcaaacagg ccaaggcgat tactgatcgc gacgcccgca gcgagctgta
    12481 taaacaggcg cagcagatca tccagcagca actgccgatc tccccgatcg cgcactcaac
    12541 ggtctaccag cccatgcgca acagcgtgaa aggcttcgag atcagcccct tcgggcgcaa
    12601 cagcttctat ggcgttagca acacgcgctg agagcggcat caacggcggg cctgagcgcc
    12661 cgccgaacac ctaataaaaa cgactgtatt cctcccccgg caaccaccgg ctgagtgttt
    12721 cgtagctgcc aaaacgttag caagaacgcg ctttccgaac gttgccgagg ctgtttcgcc
    12781 ccggcgacaa gtagtccgta taagcactcc ttgcctgata aaaaagccgt tacaggagcc
    12841 aacagatgac cttcgccctt cgcgccagca ttgttttgag cctcgctctc ggctgcgcgg
    12901 ccggccaggc aaaaccactg gtggtgtgca ccgaggcgcc gccggtcagc gcggtcagcc
    12961 gtgatgtcga gggttttacc ctcagcccca tgggctcgaa taattacgcc aaggtaaagc
    13021 ggcactgatt ctgcaagcag cactcaaaac agggctactg ccgcgtacca ggcgcgcgcc
    13081 agtggcagat cgacacaata tgccagcggc ccacgtagcc gttggcgcga atgaactgat
    13141 gcggagtaac aaaaccccat gtttgctttt atcgcacgcc gcctgggatt gctgattccc
    13201 accttcttcg gcgttacctt gctgaccttt gcgctgatcc gcatgatccc cggcgacccg
    13261 gtggaagtga tgatgggcga acgccgcgtc gacccggaaa tgcacgccca ggccatggag
    13321 cgcctgggtt tgaacaagcc gctgtatgcg cagtatttcg actatatcgg caagctggcc
    13381 cagggcgatc tgggtgagtc gctgcgcacc cgcaccagcg tctgggatga attcaccacg
    13441 ctgtttcccg ccaccctgga gttgtccctc gccgccctgc tgttcgcagg caccctgggc
    13501 ctgatcgccg gggtgattgc tgcactgaaa cggggctcac tgttcgacca tggagtgatg
    13561 ggcatctcgc tggccggcta ctcgatgccg attttctggt ggggtctact gctgatcatg
    13621 ttcttctcgg tgtggctggg ctggacgcca gtgtccgggc gtatcgacct gctctacgac
    13681 atcccgccag tcaccggttt tatgctgatc gacaccctgc tctcagatga agaaggcgca
    13741 tttgtcgacg ccctgcgcca tctgatcttg ccggccatcg tgctcggcac cattccactc
    13801 gcggtcatcg cccgcatgac ccgctcagca atgctcgaag tgctgcgcga ggactatgtg
    13861 cgcaccgccc gcgccaaggg cctgtcgccg gcccgcgtgg tgtttgtgca tggcctgcgc
    13921 aatgcgttga tcccggtgct cacggtgttc ggcctgcagg tcggcgcgct gctggccggt
    13981 gcggtactga ccgaaaccat cttctcctgg cccggcatcg gcaaatggct gatcgaatcg
    14041 atcggcgcgc gtgactaccc ggtggtgcag aacggcattc tgctgatcgc ctgcctggtg
    14101 attctggtca acttcgtggt ggacatcctc tacggcctgg ccaacccacg cattcgccat
    14161 cagcgctaag gagagcccaa tgaccacgca agccacactc gaccaatcgc tgttctaccc
    14221 ctcaccgctc aaggaattct ggcaggcctt cgcccacaac aagggcgcag tcgccgggct
    14281 gctgtttatg attgtgctgg tgatctgcgc gctgtttgcg ccgtggatcg cgccgcacga
    14341 ccccagcgag caataccgcg agtttctgct caccccaccg gcctggctgg aaggtggcca
    14401 gctgcaattt ctactgggca ccgatgaagt gggacgcgac ctgctgtcgc gattaatcca
    14461 tggcgcgcgg ctgtcgctgc tgatcggtct ggcctcggtg ctgatgtcgc tgattcccgg
    14521 catcctgctc gggctggtcg ccggcttctt tccgcgcctg ctcggcccgt cgatcatgcg
    14581 cctgatggac atcatgctgg ccctgccctc gctgctgctg gcggtggcca tcgtcgccat
    14641 cctcggccca gggttgatca acaccatctt tgccatcgct atcgtctcgc tgccgtccta
    14701 tgtgcgcctg acccgcgcag cggtgatggg cgagctgaac cgcgactacg tcactgccgc
    14761 acgcctggcc ggcgccagcc tgccgcgact gatgtttatc accgtgctgc ccaactgcat
    14821 ggcgccactg atcgtgcagg ccaccctgag cttttcctcg gcgattctcg atgccgctgc
    14881 cctgggcttt ctcggcctcg gcgtacaacc gccgacgcct gagtggggca ccatgctggc
    14941 ctcggcgcgc gactatatcg agcgcgcctg gtgggtggtc agcctgccgg gcctgaccat
    15001 tctgctcagc gtgctggcaa tcaatctgat gggcgacggg ctgcgcgatg cactcgaccc
    15061 gaaactcaag aatgcgcagt gaggaacccc gcatgagcct attggatatc aaaaacctca
    15121 gcgtgcgctt cggcgacgcc acggccatcc cggtggtcga cggcctcgac ctgtgtgttg
    15181 aaaagggcga agtgctggcg attgtcggcg aatccggctc cggcaaatcg gtgaccatga
    15241 tggcgctgat gggcctgatc gacgcccccg gcatcgtcac agccgacagc ctgcagttcg
    15301 acggcaccga catgctgcgc ctcaagggtc gccagcgtcg gcatatcgtc ggcaaagacc
    15361 tgtcgatggt ctttcaggac ccgatgaccg cactcaaccc cagctttacc gtgggtttcc
    15421 agatcgaaga agtgctgcgc caacacctga atctcaaagg tcaggccgcc cgccaacgcg
    15481 cgctgcagct gctcgaccgc gtggaaatcc ctgccgccgc cagccgcctg aatgcctacc
    15541 cgcaccaact gtccggcggc atgagccaac gggtggcgat tgccatggcg attgccgccg
    15601 agcccaaact gctgatcgcc gacgaaccga ccacagcgct ggatgtgacc attcaggcgc
    15661 agatcatgga cctgctggtg ggcctgcaac gcgaacagaa catgggcctg gtgctgatca
    15721 cccacgacct ggccgtggtg gctgaaaccg cgcaacgggt ttgcgtgatg tatgccgggc
    15781 aagcggtgga gataggcaac gtgccgcagc tgttcaccgc ccccagccac ccctacaccg
    15841 aggcgctgct caaggcgatt cccgaacaca gccagggcca gcgccgcctg accaccctgc
    15901 ccggcatcgt cccgggccgc tacgaccggc cagtgggctg cctgctgtcg ccacgctgcc
    15961 cctacgccca ggcgcactgc cgtgaacaac ggccgaccct ggatgcccat gaacgtggcg
    16021 cggcgcgctg cttctacccg ctcaacctga ctgcggaggt ggcctgatga gcgccgtcct
    16081 gactgctcgc gacctcaccc ggcactacga aatctcccgc ggcctgtttc agcccagcgc
    16141 ccaagtgcag gcgctgaatg gcgtgtcctt tgaactgcaa gccggcaaga ccctggcggt
    16201 ggtcggcgag tccggctgcg gcaaatccac cctggcccgc gccctgacgc tgatcgagga
    16261 gcccagctcg ggctcgctgc agatcgccgg ccaggaggtg actggcgcca acaaggccca
    16321 gcgcaagcag ctgcgccgtg atgtacagat ggtctttcag aacccctatg cttcgctcaa
    16381 tccacggcag aaggtcggcg atcagctggg cgagccgcta ctgatcaaca ccggcctgtc
    16441 gcgcctggaa cggcgcgagc gggtgcagac aatgatgcag caggtcggcc tgcgccccga
    16501 gcattaccag cgctacccac atatgttctc cggcggccag cgccagcgta tcgccctggc
    16561 ccgggcgatg atgctgcaac ccaaggtgct ggtagcggac gaaccgactt cggcgctgga
    16621 cgtgtcgatc caggcccagg tgctcaacct gtttatggac ctgcaggagc agttcaacac
    16681 cggctacgta tttatctcgc acaacctctc ggtggtgcgc cacgttgccg atgatgtgct
    16741 ggtgatgtac ctcggccgcc cggtggaaat gggccccagc gagctgatct acagccgccc
    16801 gctgcaccct tatacccagg cgttgctgtc ggcgacgccg actatccatc ctgatccgag
    16861 caaaccgaag atcaagatca gtggcgagct gcccaacccg ttgtcgcccc ccagcggttg
    16921 cgccttccac caacgctgcc cgcatgccac tgaccgttgc caggttgaac agccggcgct
    16981 gcgcctgctt gatgcacggc aggtggcttg ccaccatgcc gagcagatca atagctaaat
    17041 gatgtgagcc ctggccagcc ggggctcgct atggcgcagc cgtacctggg tagaacagcc
    17101 gatagcccag caggccaatg gcctcgttaa gcagcaggcc gttctgccag actgccttgc
    17161 tttccggtaa ccagccgcca gcaggccggc cattgggcgt accgagaaag cccatcggcg
    17221 cgctgaccac ctgcaaaccc gcctgttcga aactccagcg cgacctgggc atatgccagg
    17281 cctgggtcac cagcaccacg cgggtaatcc ccgcctgttt cagcagctca gcgctgtggg
    17341 tcgaattctc ccaggtggtg cggctcaagc cttcctgcca acgcaccggc acgtcgtaat
    17401 cgcgtgccag cacctcggcc atcaacgccg cctcgctggg cggtacgcca tagtgcaacc
    17461 cgccactggt tagcagcggc aagcccgaag ccttggccag ccgcgccgcg aaacgcaccc
    17521 gttccaacgc catcaggctg ggctgatcag cgccccaacc agggtcagcc agttcacgcc
    17581 cgccacccag aaccacaatc acttccgcct gccgagccag atcagtccat tgccgctcat
    17641 tcaaggccgg ctcggtttcc agtatccgcg ccgaatactc gaccaccacc ggcaaactaa
    17701 acagccacat accgcccaag cccgccacaa aacaccccag cgccagacgc ggaaaacgcc
    17761 gacgcagcca ccaggcggcc agcagcaaaa gcaggaacag gcctggcgga aggaagaact
    17821 gtttgaggat gtagcgaatc ggcattgggc acctccgtgg gtgcgcgcag cctaccggtg
    17881 atttgtcacg cgaaacaact gcgagccgca ggctttgcag gcctgtacgc cattgccgga
    17941 ctgccagccc gcctgggtgc cgcacagcgc acaacacagc tgcaatcggc gtttaagcag
    18001 cggtttgatc ttgccgggca gcgccgcggt gggtttgtgg ccggagcggc cgaccttgcg
    18061 tgagctcggc aactgttcga tctgcgccat ttcctgagcc gcggcatgcc agccgcgcag
    18121 ccagcccaaa tcgcgatcca ggtaggcgcg aatcagctcc atttccgccg ccgtcaagcc
    18181 gcgcaactcc aactcctgct gcggttgagc ggacagtacg ctcaggttgt ccgcctcatc
    18241 caacgccatg gcaagacgtt gcagcagccg ctcatagagc ccgccccccg ctgcaccacg
    18301 ccgaaactcc gccatcctgc acctcgcgcc ggagcaggct gctgctccat atagcaagct
    18361 tagcgttcgc gctttaaccg gcagtgcctc gcgacaaacg gcgcgaccgg ctagggcggc
    18421 cggcgaggcg cggctggcta agtgcggccg ggcaggccgc cgtagctgga gcgcggcgca
    18481 atcagggttt ccctgaataa gcggggctca tgtatgctac agcgtttccc gcaagccctt
    18541 attcccagcg ccggtagcca tgcaagaaca gtactcgccc cgtgaaattg aagccgccgc
    18601 gcagtcccat tgggacgcgc agaaatcctt tgtagtgagc gaacagccgg gcaaggagag
    18661 cttctactgc ctgtcgatgt tcccctaccc gagcggcaag ctgcacatgg gccatgtgcg
    18721 caactacacc atcggcgacg tgatcgcccg ctaccagcgc atgctcggca agaacgtact
    18781 gcagcccatg ggctgggacg ctttcggcat gccagcggaa aacgccgcaa tgaagaacca
    18841 ggtcgcaccg gcgaagtgga cctacgaaaa catcgaatat atgaagaccc agctgaagtc
    18901 gctgggtctg gccgtggatt ggactcgcga agtcaccacc tgcaagccgg attactaccg
    18961 ttgggaacag tggctgttca cccgcctgtt cgaaaagggc gtgatctacc gcaagaacgg
    19021 cagcgttaac tgggacccgg tcgaccagac cgtactggcc aacgagcagg tgatcgacgg
    19081 ccgcggctgg cgttccggcg cggtaatcga aaagcgcgaa atcccgatgt actacttcaa
    19141 gatcaccgct tacgcggaag agctcttgag tagcctggac gaactggatg gctggccgga
    19201 acaggtcaag accatgcagc gcaactggat cggcaagagc ttcggcgccg acgtggtgtt
    19261 cgactatgac caagccagcg tcggtatcga cggtcagctc aaggtctata ccacccgccc
    19321 cgacaccctg atgggcgcca cctacgtcgc cgtggccgcc gagcatccgc tggcgcaacg
    19381 tgccgccgag agcaatccgg ctatcgctgc ctttatcgcc gaatgcaaag ccggctccgt
    19441 ggccgaggcc gacatggcca ccatggagaa gaaaggcgtt gccaccgggc aattcgttat
    19501 tcacccgctc tccggcgaca agctgccagt attcgtcgcc aactatgtgt tgtggggcta
    19561 cggcgaaggc gcggtcatgg ccgtgccggc gcacgatgag cgcgacttcg aattcgccaa
    19621 caaatatgac ctgtcgatca agcaggtcta tgccggcgaa ggcaaagaat acgacgccac
    19681 tcaatggcaa gactggtacg gcgacaaagc tggcctcagc accattggct ccggcaaata
    19741 cgacaatctg gacttcactg cggcttttga cgccatcgtc gccgatctgg aagccaacgc
    19801 ccacggcgcg cgcaaaaccc agttccgcct acgcgactgg gggatcagcc gccagcgtta
    19861 ctggggctgc ccgatcccga ttatccactg cgatacctgc ggcgacgtac cggtgccgga
    19921 agaccagctg ccagtcgtac tgccagaaga cgtagtgccg gatggcgccg gcagcccgct
    19981 ggcgcgcatg cctgaattct acgaatgcag ctgcccgaaa tgcggcgcac cggccaagcg
    20041 cgaaaccgac accatggaca ccttcgttga gtcgtcctgg tattacgccc gttacgcctc
    20101 gccacattac accggcggca tggtcgaccc tgaagcggcc aaccactggc tgccggtgga
    20161 tcaatacatc ggtggtatcg aacacgcgat cctgcacctg ctgtacgcgc gcttcttcca
    20221 caaactaatg cgcgacgaag gtcttgtaag ctccaatgag ccattcaaga acctgctgac
    20281 ccagggcatg gtggtcgccg agacctacta ccgcaccctg gaaaacggcg gcaaggactg
    20341 gttcaacccg gccgatgtcg agctggagcg tgacagcaaa gccaaggtca tcagcgccaa
    20401 actgaaaagc gacggcctgc cggtggaaat cggcggcacc gagaagatgt ccaagtcgaa
    20461 gaacaacggc gtcgacccac agtcgatgat cgatcaattc ggcgccgaca cctgccgcct
    20521 gttcatgatg ttcgcctcgc cgccggatat gagctgcgaa tggtccgatt ccggcgttga
    20581 aggcgctaac cgcttcctgc gccgcgtctg gcgcctggcg caaaaccacg tcaatcaggg
    20641 catcgtaggt gcactcgacc tggccagcct ggatgacgag cagaaggctg tgcgccgggc
    20701 gattcatctg gcgatcaagc agtcgagctt cgatattggc caacaccaca aattcaacac
    20761 cgccatcgcc caggtgatga cgctgatgaa cgtgctggaa aaagccgcac aaaccacagc
    20821 tcaggactgc gccctaatgc aggaaggtct ggaagccgtg gccctgctgc tggcgccaat
    20881 tacgccgcat atcagccatc agctgtggca ggaactgggc catgacgaag ccatcatcga
    20941 cgcatcctgg ccgcaggtgg atgaaagcgc gctggtgcag gacagcctga ccctggtgat
    21001 ccaggtcaac ggcaagctgc gcgggcagat tgaagtcccc gccagtgcca gccgcgaaga
    21061 tgtcgaagcc actgcccgca gcaacgagaa tgtgctgcgt tttaccgaag gcctgagcat
    21121 tcgcaaggtc atcgtggtgc caggcaaact ggtcaacatt gttgccaact gactagtcgc
    21181 caactgatta agctcggcgc gaccgcacaa cggcgcgccg caagacacgc caaggggata
    21241 aaagatgatc aaacggaatc tgttggtaat tggcctggcc accctgctca gcgcttgcgg
    21301 cttccag
//
LOCUS       DASRUM010000035         8366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1694015, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000035 DASRUM010000000
VERSION     DASRUM010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8366
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694015"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1210)
                     /gene="rssB"
                     /locus_tag="VFF22_02730"
     CDS             complement(26..1210)
                     /gene="rssB"
                     /locus_tag="VFF22_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HZX15769.1"
                     /translation="MHKTSATLLIIDDDDVVRASLAAYLEDSGFHVLQAANGLQGLEV
                     FHAESPDLVICDLRMPQVDGLELIRRINALQVETPVIVVSGAGVMSDAVEALRLGAAD
                     YLIKPLADLAVLEHSVRRALDRANLRLENQRYRDELETANRELQASLHLLQEDQNAGR
                     QVQMNMLPVTPWQADSLNFAHQIIPSLYLSGDFVDYFRVDDSRIAFYLADVSGHGASS
                     AFITVLLKFMTTRLLYESRRGGNLPDFKPSEVLGHINRGLINCKLGKHVTMLGGVIDQ
                     ANNRLTYSIGGHLPLPVLYSDGQARYLEGRGLPVGLFVEAEYNDLELELPEAFSLTLL
                     SDGILDLLPGDTLKDKETLLPQLISSAGGTLDGLRKAFGLANLGDMPDDIALLVLSRN
                     LA"
     gene            1427..1726
                     /locus_tag="VFF22_02735"
     CDS             1427..1726
                     /locus_tag="VFF22_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZX15770.1"
                     /translation="MSQNDRAYSEKRDYIRMRLEAPVTLHHDGKEIPALCLDLSSTGM
                     QLEAESAVKMGDKVRIHIASDHNELRGLDAQAEVVRVSTMDDGRQALGLAILSMS"
     gene            complement(1787..2488)
                     /locus_tag="VFF22_02740"
     CDS             complement(1787..2488)
                     /locus_tag="VFF22_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004659844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HZX15771.1"
                     /translation="MRVTGASWIERLSLLIACTGMLLLPALAQANEEDPWQGYNRFMF
                     RVNDNVDTYTLKPLAKGYQAVTPQFLEDGVHNVFRNIGDVGNLANNLLQGKLHDAGVD
                     GGRLIFNTTFGLLGFFDVGSTMGLQRSDEDFGQTMGAWGLNSGPYVVLPLLGPSTVRD
                     AFGKVPDSMLSAPAFIDHVPTRNVVRGVQVVDVRASLLKAERMVSGDKYIFIRNAYLQ
                     NREFKVRDGEVEDDF"
     gene            2721..3431
                     /locus_tag="VFF22_02745"
     CDS             2721..3431
                     /locus_tag="VFF22_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZX15772.1"
                     /translation="MQTPATSPAFTAVLFSLSGCLVDFGARTIPLTLQRLYPKAANHQ
                     SLQQALGREPLPEEWQAYQQALCETACEHAEATPAALTLLEQLHQQAVPCAWLDELPR
                     DASISLASALPNWLPGISSPSTRPWPAPDAIWRSLMELQVDQLEGCVLVSGEPRLLKA
                     ALNAGIWTIGLAISGPLCGLAPSDWQSLPMGERDRLRSQATLSLYRLGVHSVIDQLSD
                     LPTCLADLSLRRHKGEKP"
     gene            3580..3846
                     /locus_tag="VFF22_02750"
     CDS             3580..3846
                     /locus_tag="VFF22_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003239904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HZX15773.1"
                     /translation="MPASRLQQQLQDLRDQLAEQPPLTEEERAELFVLTQEIELQLAR
                     EAAAAPDATLIDGVNLAVERFEASHPTLAGTLRNIVQSLANMGI"
     gene            complement(3895..4725)
                     /gene="queF"
                     /locus_tag="VFF22_02755"
     CDS             complement(3895..4725)
                     /gene="queF"
                     /locus_tag="VFF22_02755"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552892.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HZX15774.1"
                     /translation="MHPAAEDSPLGKSSEYIATYTPSLLFTIPRAAKWAELGLSAETL
                     PYQGVDFWNCFELSWLLPSGKPVVAMGEFAIPADSPNIIESKSFKLYLNSLNQSVFNS
                     ADELVAVLTQDLSAAAGKPVGVRVRSLAEVTADGVQAAPGICIDELDVAISNYAQPQP
                     ELLRCDAARAVEESLYSHLLKSNCPVTGQPDWGTLVVQYRGLALDHASLLAYLVSFRQ
                     HADFHEQCVERIFLDLQRLLKPEHLTVYARYVRRGGLDINPYRSSGPISPDNQRLVRQ
                     "
     gene            complement(4821..5519)
                     /gene="pyrF"
                     /locus_tag="VFF22_02760"
     CDS             complement(4821..5519)
                     /gene="pyrF"
                     /locus_tag="VFF22_02760"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HZX15775.1"
                     /translation="MSACQTPIIVALDFPTRDAALALADQLDPKLCRVKVGKELFTRC
                     GPEIVNVLAGKGFEVFLDLKFHDIPNTTAMAVKAAAEMGVWMVNVHCSGGLRMMAACR
                     ETLDKLAGVKPLLIGVTVLTSMEREDLAGVGLDIEPQAQVLHLAGLAAQAGLDGLVCS
                     AQEAQALKAAYPALQLVTPGIRPAGSAQDDQRRILTPRQALDVGSDYLVIGRPISQAA
                     HPAQALAAVVAELA"
     gene            complement(5906..6002)
                     /gene="ffs"
                     /locus_tag="VFF22_02765"
     ncRNA           complement(5906..6002)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFF22_02765"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(6103..7737)
                     /locus_tag="VFF22_02770"
     CDS             complement(6103..7737)
                     /locus_tag="VFF22_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX15776.1"
                     /translation="MLRVFADLGFRWKIALPIMLLAVLLVSMGVLGMRGITQVADSST
                     KLTNRFLPGISLLLNADRDLYQAFVAERSLLDEGVGVHAEALTAAHAENLQQAYDRVH
                     KFADMQPGSEAMQLVAQFDLGFARWKSTSQKVLQLVASDPQAASVLSFGDSEEQFEVM
                     RTVIDKLGEMDDAAANAQGVAAIQLGETLSLQQGVIITIGLGVCLVLVVGFPILVTGP
                     LHHLLNRIEQIADGDGDLRVRLDVLSNDELGKLSHAFNRFLDKLQPLIKEVGRVTGEV
                     ESSAQSLAGMAAANDRLISSEHAAVDQVSTAATEMSSAVHEVARNAQNAADAARSAEA
                     QSREGAQVVGATINSIRQLAQEVESASATIQTLEQEAVNIGAVLAVIRGIAEQTNLLA
                     LNAAIEAARAGEQGRGFAVVADEVRALAARTQESTKDIQVMIERLQIGVQNAVKATHS
                     GSSKARQSVEQAAGVDQALTDTSDSVQRINDMTAQIATACEEQSSVTEEIARNISDIR
                     DLSNEAAQTSEQSAQASQRLSELSHGLSALVGRFRV"
     gene            7896..8174
                     /locus_tag="VFF22_02775"
     CDS             7896..8174
                     /locus_tag="VFF22_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZX15777.1"
                     /translation="MAMTKDQLISDIAEAIDTPKSTVRAALEQLSEIVSDALENGDEI
                     TLSGIGKFKVSERPARTGRNPQTGQALQIAAKKVVKYVPAKALSDAIN"
     gene            complement(8275..>8366)
                     /gene="rrf"
                     /locus_tag="VFF22_02780"
     rRNA            complement(8275..>8366)
                     /gene="rrf"
                     /locus_tag="VFF22_02780"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1990 a         2609 c         2179 g         1588 t
ORIGIN      
        1 attctaccgg ggcttatccc agggttcatg caaggttcct gcttaacacc agcaaggcga
       61 tatcatccgg catatccccc agattggcca gcccaaaagc cttgcgcaag ccatccaggg
      121 tgccgcccgc ggaactgatc aactgcggca acagggtttc cttgtctttc aaggtatcgc
      181 ccggcagcag atcgaggatg ccgtcggaga gcagcgtcaa gctgaaggcc tccggcaatt
      241 caagttccag atcgttgtat tcggcctcga caaacagccc aaccggcagg ccgcggcctt
      301 ccaggtaacg cgcctgacca tcgctgtaga gcaccggcag cggcagatga ccgccaatgc
      361 tgtaggtcag gcggttgttg gcctgatcga tcacgccgcc aagcatggtc acgtgcttgc
      421 ccagcttgca gttgatcagc ccacggttga tatggccgag cacttcagag ggtttgaaat
      481 ccggcagatt gccgccgcgc cgcgactcat acagcaggcg cgtggtcata aacttcagca
      541 gtacggtgat aaatgccgaa gaagcgccat gcccggaaac atcggccaga taaaaagcga
      601 tacggctgtc atcgacgcgg aaatagtcga caaagtcgcc cgacaggtac agcgacggaa
      661 tgatctggtg cgcgaaattc aggctatcgg cctgccacgg cgtcaccggc agcatgttca
      721 tctgcacctg gcgaccggcg ttctgatcct cctgcagcag gtgcaggctg gcttgcagtt
      781 cgcggttggc ggtttccagc tcgtcgcggt agcgttggtt ttccaagcgc aagttggcgc
      841 gatccagcgc acgacgcact gagtgctcaa gcacggcaag atcggccagc ggtttgatca
      901 ggtaatcggc agcgcctagg cgcaaggcct cgacggcatc gctcatcacg ccagcaccgg
      961 acaccacgat caccggggtt tccacttgca gggcattgat acggcggatc agctcgagac
     1021 catcgacttg cggcatgcgc agatcgcata tcaccagatc cggcgactcg gcatgaaaga
     1081 cttccagccc ctgcaagcca ttggcggcct gcagcacatg aaaaccactg tcttccaggt
     1141 aggctgcgag gctcgcacgc actacgtcgt cgtcatcgat tatcagcagc gtggcactgg
     1201 ttttgtgcat gaggtatcca ggcaaactgc gccgggtatg ggttggctta gtacgtcagg
     1261 gcaaccgcct ggacgcgcga actcggcttg cttcaaggcg cagacggtac tcccatccgc
     1321 aacgtgattc aagcgcatgc cggtcgtcgc cgtacgactt tacacctcaa ataggccaag
     1381 gttataagag ctacaggaca gcccccataa caagaggaaa aagcccatga gccaaaatga
     1441 tcgtgcctac agcgagaaac gcgattacat ccgcatgcgg ctagaagccc ccgtcaccct
     1501 gcaccatgac ggcaaggaaa ttcccgcact gtgtctcgat ctgtccagca ctggaatgca
     1561 gctggaggct gaaagcgcgg tgaagatggg tgacaaggtg cgtatccaca ttgcctcgga
     1621 ccacaacgag ctgcgcggcc tggatgcgca agccgaagtg gtgcgcgtca gcacgatgga
     1681 cgacggccgc caggcattgg ggctggcgat cctttccatg agctgagcat actcggaaac
     1741 aaaaaaggcg gcctactaag ccgccttttc tatatgtgcc gacgagtcag aagtcgtctt
     1801 caacctcgcc atcacgcacc ttgaattcgc gattctgcag gtaagcattg cggataaaaa
     1861 tgtacttatc gccgctgacc atacgctcgg ctttaagcaa gctggcccgc acatcaacaa
     1921 cctgaacgcc acgcaccaca ttgcgcgtcg gcacatgatc gataaacgca ggcgcactca
     1981 gcatgctgtc cggcaccttg ccgaaggcat cacgaacggt gcttgggccc aacagcggta
     2041 acaccacata aggcccgcta ttgagccccc aagcgcccat ggtctgaccg aaatcctcat
     2101 cgctgcgctg caggcccatg gtgctaccca catcaaagaa gcccagcagg ccgaaggtgg
     2161 tgttgaagat caaccgaccg ccatcaaccc cggcgtcatg caacttgcct tgcagcaggt
     2221 tgttagccaa gttgccgaca tcaccgatat tgcggaagac gttgtgcacg ccgtcttcca
     2281 gaaactgcgg tgtcaccgcc tggtagccct tggccaatgg cttgagggta taggtatcaa
     2341 cgttatcgtt gacccgaaac ataaagcggt tgtaaccctg ccatgggtct tcttcgttgg
     2401 cttgcgccag tgcaggcagc aacagcatgc cggtacaggc aatcaacagg ctcaatcgtt
     2461 caatccagct cgcaccggtc acacgcatca cagtcgctcc ttgaaaatgc tcaggcagcc
     2521 acaggcggcc aaattaggcc gcgaagtata aggcggcagc ccactaaata ggcagcatag
     2581 cggacgaaca aatagctgaa aaatcttctc tcctgtgccg cgcagttcag cgattgcagc
     2641 aatgcgaact gaaacaaagt cttcacctgc ctgtaacctg aaaccgttag cctggactag
     2701 accattttca aggacggccc atgcaaacgc ccgccacctc gcccgccttc accgccgtcc
     2761 tgttcagcct gtccggctgc ctggttgatt tcggcgcacg caccattccc cttaccctgc
     2821 aacgcctcta ccccaaagca gccaatcacc agagcttgca gcaggccctg ggccgtgaac
     2881 ccttgccaga agagtggcaa gcctatcagc aggctctctg cgagactgca tgcgagcatg
     2941 ccgaagccac accagccgcc ctgaccctgc ttgaacagct gcatcaacaa gcggtgccct
     3001 gcgcctggct ggatgaacta ccacgcgacg ccagcatcag cctggccagc gccctaccaa
     3061 actggctgcc cggcatatcc agccccagta ctcgtccatg gccagccccc gacgccattt
     3121 ggcggagctt gatggagctg caggttgatc aacttgaagg ctgcgtactg gtcagtggcg
     3181 aaccacgtct gctaaaagcc gcactgaatg ctggcatctg gaccatcggc ctggccatca
     3241 gtggcccgct ctgcggcttg gcaccatctg actggcagag tctgccgatg ggtgaacgcg
     3301 accgcctgcg tagccaggcc acattgagtc tgtaccgttt gggcgtgcac tcggtaatcg
     3361 accagctcag cgacctgccc acctgcctgg cagacctcag cctgcgtcgc cacaagggcg
     3421 agaagccatg agcaagcgcc aaactgccgg ctaaatcaat gcctcacgct tgatccaaag
     3481 caagcaaagg cggcgggctt ggattaacct gaaggtatgc aaaacctttc gcccacgcgc
     3541 aaggtcagtc aatctgtcga ttacccaagg gagaattcca tgcctgcaag ccgcctgcag
     3601 caacaactgc aagacctacg cgaccaactg gccgagcagc cgccactgac tgaagaagaa
     3661 cgcgccgagc tatttgtact gacccaggaa attgaattgc aactggcccg tgaggctgca
     3721 gctgcccctg acgcaaccct gattgacggg gtcaacctcg cggtggagcg ctttgaagcc
     3781 agccacccga ccctcgccgg caccctgcgc aatatcgtgc agagcctggc caacatgggt
     3841 atttaaacgc acccagcact aacgaaaacc ccagcactta gctggggttt tgcattactg
     3901 gcgcaccaaa cgctgattat ccggactgat gggtccgctg ctgcgataag gattgatatc
     3961 caacccgcca cgccgcacat aacgcgcata caccgtcagg tgctcaggct tgagcaggcg
     4021 ctgcaggtcg agaaagattc gctccacgca ctgctcgtga aaatccgcat gctggcgaaa
     4081 gctcaccagg taagccagca ggctggcgtg atccagcgcc agacctcggt actgcaccac
     4141 cagcgtgccc caatccggct gaccggtaac cgggcagttg gatttgagca gatgactgta
     4201 caggctctct tcgaccgctc gcgcggcatc acagcgcagc agctcaggct gcggctgcgc
     4261 atagttgctg atggctacat ccagctcgtc aatgcagatg cctggcgcgg cctgcacacc
     4321 gtcggccgtc acctccgcca ggctgcgcac acgcacgcca actggcttgc cggcagctgc
     4381 cgagaggtcc tgtgtcagta ccgccaccag ttcgtcagcg ctgttgaaca ccgactgatt
     4441 tagcgagttg aggtacagct tgaaggattt cgactcgatg atatttggag agtcggcggg
     4501 aatggcgaac tcgcccatag ccactaccgg cttgccggac ggcaacaacc aggacaactc
     4561 gaaacagttc cagaaatcca cgccctgata cggcagcgtc tcagcgctca ggcccagctc
     4621 ggcccatttc gcggcacgcg gaatcgtgaa caacaaagac ggggtgtagg tagcgatgta
     4681 ttcgctggat ttgcccagtg gcgaatcttc ggcggcggga tgcatggaga aaacctggct
     4741 agtcgaaacg cggaaattgt atacaacttt cagagcagat tcagttgggc aagaccaatg
     4801 tcggccttgc cgcagcagca tcaggccagc tcagctacca ccgctgccag cgcctgggcg
     4861 ggatgggcag cctggctgat cggccggccg atcaccagat aatccgagcc tacatccaac
     4921 gcctgacgcg gagtcagaat acggcgctgg tcgtcctgag cgctgccggc cggacgaatc
     4981 cccggcgtca ccagttgcag agccgggtaa gcagccttca gcgcctgcgc ttcctgagcc
     5041 gaacacacca ggccatccag acccgcctgc gccgccagcc cagccaggtg cagcacctgc
     5101 gcctgcggct cgatatccag gccaacgccg gccagatctt cgcgttccat gctggtcagc
     5161 acggtcacac cgatcagcag cggcttgact ccggcaagct tatccagggt ttcacggcaa
     5221 gccgccatca tgcgcaggcc gcccgagcag tgcacattga ccatccacac gcccatctcg
     5281 gccgcggctt tgacggccat ggcggtggta ttgggaatgt cgtgaaattt caggtcgagg
     5341 aacacctcga aacccttgcc agccagcacg ttgacgatct ccggcccgca acgggtgaac
     5401 agctccttgc ccacttttac ccggcacagc ttgggatcaa gctgatcagc caatgccaga
     5461 gcggcatcac gggtcgggaa atccagggca acgatgatcg gggtctggca ggcggacatg
     5521 ggcaggctct cgggcaagac aaattcggcg cgcattgtag cggatgcgca gcacgcttgc
     5581 cgctccagtg atccacccgc ctcgctcaaa cttgcagcta ctcggcagcc tcagcagcac
     5641 acgcacatcc tgcgccgcaa acacctgacg aaagctgaac gctgcgcgct agcgctctcc
     5701 tcgcgcaaca acgccagacg ctaggcagtg gccagcatac gacaggcgct actctgacgg
     5761 gcagtgctgt gaacaccaat gaatcgagca ctgaatggga ttgccagcac agtacaccac
     5821 tgaggtgtac ctaaacgctg ttacacttcg agactttttg actgataaat ccttgcagaa
     5881 tcaaggattt aggatatgga ggcaacccca ccagccacac ctcggcacac aatgtcaccg
     5941 ctgcggctgc tcccttccag gcctgaccgg gttcacggct gattgttgcg aggggaccgg
     6001 cagggccacc ataacgcagc tcgccagaag cgagccgcgc cattgtaccg gcttgaatcg
     6061 aagttacaac cgtttcaaac acttagccca tcgaaacgca cactacacac gaaaccgacc
     6121 aaccagcgcg ctcaaaccat gcgacaactc agatagacgt tgactagcct gggcactctg
     6181 ctcggaggtt tgagcagctt cattagaaag atcgcgaata tcactgatat tgcgtgcaat
     6241 ttcttcggtc acgctgctct gctcttcgca ggctgttgcg atctgtgcgg tcatatcatt
     6301 gatacgctgc accgagtcac tagtatcagt aagcgcctga tccactccgg ccgcttgctc
     6361 gacactttgc cttgccttgc tactcccaga atgggtggct ttaaccgcat tctgtacacc
     6421 gatctgcaag cgctcgatca tcacctggat gtctttggtc gactcctgcg tacgcgcagc
     6481 caatgcacgc acctcatcag ccacaaccgc aaagccgcgc ccctgttctc cagcacgagc
     6541 cgcctcaatg gccgcattta gtgccaacaa gttagtttgc tcagcaatac ccctgataac
     6601 agccagtacc gcacctatat taacggcctc ttgctcaagg gtctgaatgg ttgccgatgc
     6661 gctttcaact tcttgcgcta actggcgaat cgagttgatc gtggccccta caacctgcgc
     6721 accttcacga gattgcgcct cagcgctacg cgcagcgtca gctgcatttt gcgcattgcg
     6781 agccacttca tgtacagcag agctcatttc cgttgcagca gtgctgacct gatcgaccgc
     6841 agcatgctca ctactaatta accggtcatt agcagcggcc attcctgcca gactttgcgc
     6901 agaactttcg acctcccccg tgacccgccc aacctccttg atcaatggct gcaatttatc
     6961 aagaaagcgg ttgaacgcat ggctgagctt tcccagctca tcgttggaca aaacgtccaa
     7021 gcgcactcgc agatcaccat caccatcagc aatctgctca atacggttaa gtaggtgatg
     7081 cagaggtcca gtcacgagaa tgggaaaacc aaccactaat acaaggcaaa cacccaaacc
     7141 aatggtaatg atcacaccct gctgcaagct caaggtctcg cccaactgaa tagcagccac
     7201 cccttgagca ttggctgccg catcatccat ctcgcccaac ttatcaatga ctgtacgcat
     7261 cacctcgaat tgctcttcac tatcaccaaa gctcaacaca ctggctgctt gtggatcact
     7321 agccaccaac tgcaagactt tttgcgacgt tgatttccag cgtgcaaagc ccaaatcaaa
     7381 ctgcgcaacc agctgcatcg cttcactacc aggctgcatg tccgcaaatt tatgtacacg
     7441 gtcataagcc tgctgcagat tttctgcgtg tgccgcagtc agtgcttcag catgcacacc
     7501 cacgccttca tcaagtaaac ttcgctcagc aacaaaagcc tgataaagat cacggtctgc
     7561 attaagtaac agactaatgc ccggcaagaa acgattagtt agcttcgtac ttgagtctgc
     7621 aacttgagtg atgcctcgca tccccagaac ccccatagaa accaacaaaa ccgccagcag
     7681 cataatcggt agtgcaatct tccaacgaaa ccctaaatca gcgaagacac gaagcatgct
     7741 ggctctccag gacaatggcc aagtagcatc agcatagact actgacggca gagcagaata
     7801 aagctctgcc tactgcctca cctcacctta aaagcttatg acacactcgc agtacgacct
     7861 ttatcggccc catacacgaa ccaaggaaca gcaccatggc gatgacaaaa gaccaactga
     7921 tcagcgatat tgcagaagct atcgatacgc cgaaaagcac cgtacgcgcg gcacttgagc
     7981 agctgagcga aattgtcagc gatgcactgg agaacggcga tgagattact ctgtcgggga
     8041 ttggaaaatt caaggtgagc gaacgccctg cgcgcaccgg ccgcaacccg caaacgggtc
     8101 aagccctgca aatcgcagcc aagaaagtcg tcaaatacgt acctgcaaaa gcactgagcg
     8161 acgcaatcaa ctgagaatta agcgcgctct tgcgagcgcg gtttttcagc attatttatc
     8221 gcgcacaaag taaaaccccc gaccgttttc acgatcgggg gttttggtat aggtgcttga
     8281 cgatgaccta ctctcacatg gggaaacccc acactaccat cggcgatgca tcgtttcact
     8341 actgagttcg ggatgggatc aggtgg
//
LOCUS       DASRUM010000036         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_850866, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000036 DASRUM010000000
VERSION     DASRUM010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850866"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1141
                     /locus_tag="VFF22_02785"
     CDS             134..1141
                     /locus_tag="VFF22_02785"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HZX15778.1"
                     /translation="MINFHQVHKAYRVSGQDIPALQPSDLQIARGEVFGIIGHSGAGK
                     STLLRLINRLEQPSGGRIEIDGEDVTALDATGLRRFRQQVGMIFQHFNLLSSKTVADN
                     VALPLKLAGELPRSAINARVTELLARVGLQDHANKYPAQLSGGQKQRVGIARALATEP
                     KILLCDEATSALDPQTTASVLQLLAEINRELNLTIVLITHEMDVIRRVCDRVAVMDAG
                     VIVESGSVAEVFLHPQHATTKRFVQEAEQIDEHEQRDDFAHVAGRILRLTFQGEATYA
                     PLLGTVARETGVDYSILAGRIDRIKDTPYGQLTLALTGGDLDAALARFEAADVHLEVL
                     R"
     gene            1141..1818
                     /locus_tag="VFF22_02790"
     CDS             1141..1818
                     /locus_tag="VFF22_02790"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008018443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HZX15779.1"
                     /translation="MEALLTRLLPNVDWAEIGYASLDTLSMLGGSMLFTVLLGLPLGV
                     LLFLTSPRQMFEQKAFYGFLSLLVNILRSVPFVILLIVMIPFTVIVTGTSLGVAGAIP
                     PLVVGATPFFARLVETALREVDRGIIEASQAMGATTRQIVVNALLPEALPGIIAAITV
                     TAITLVSYTAMSGLIGGGGLGDLAVRYGYQRYQPDVMAVTVILLLVLVQILQTVGDKL
                     VVHFSRK"
     gene            1898..>2008
                     /locus_tag="VFF22_02795"
     CDS             1898..>2008
                     /locus_tag="VFF22_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012721513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     protein"
                     /protein_id="HZX15780.1"
                     /translation="MKKLIAAFAALAAFSAQAESLSVAATAVPHAEILEFV"
BASE COUNT          372 a          658 c          597 g          381 t
ORIGIN      
        1 agcagtcagc catctaatgc aatttagcta taaccatagg tctacaaaga tttttgcgac
       61 ctaaacagcg ccgggtagac tagccgccgt ttcggcctac ccggcagccc gaaaaaccca
      121 aaaggtttat cccgtgatca actttcatca ggtccacaag gcgtatcgcg tcagtggtca
      181 ggacatcccc gcgttgcaac cgagtgatct gcagattgcc cggggcgaag tgttcggcat
      241 catcggccac tctggcgccg gcaaaagcac cctgctgcgt ctgatcaacc gcctggagca
      301 gccttccggc gggcgtatcg agatcgacgg cgaagacgtc acagcgctcg atgccacagg
      361 cctgcggcgc ttccgccagc aggtcggaat gatcttccag cacttcaatc tgctgtcttc
      421 caagaccgtc gccgacaacg tcgctctgcc gctgaaactg gccggcgagc tgccgcgcag
      481 tgcgattaac gcacgcgtga ctgagttgct ggcccgggtc ggcctgcagg atcacgccaa
      541 taagtacccg gcgcagctct ccggcgggca gaaacagcgt gtcggcattg cccgcgccct
      601 ggccaccgaa ccgaagattc tgctgtgcga cgaggccacc agcgcactcg acccgcagac
      661 caccgcctcg gtgttgcagc tgctggctga aatcaaccgc gagctgaacc tgaccatcgt
      721 gctgatcacc cacgagatgg acgtgatccg ccgcgtctgc gaccgggtgg ctgtgatgga
      781 tgccggcgtg attgtcgaat ccggatcggt ggccgaggtg tttctgcatc cgcagcacgc
      841 caccaccaag cgctttgtgc aggaagccga gcagatcgac gagcacgaac agcgcgatga
      901 ttttgcccat gtcgccgggc gcatcctgcg tctgaccttc cagggcgaag ccacctacgc
      961 accgctgctg ggcaccgtgg cacgggaaac tggcgtggat tacagcatcc tcgccgggcg
     1021 catcgaccgc atcaaggaca ccccctacgg ccagctgacc ctggccctga ccggcggcga
     1081 tctggacgcc gcgctggcgc gctttgaagc ggccgacgtg catctggagg tgctgcgcta
     1141 atggaagccc tactgactcg cctactgccc aacgtcgatt gggcggaaat cggctatgcc
     1201 agcctggaca ccctgagcat gctcggcggc tccatgttgt ttaccgtgct gctcggcctg
     1261 ccgttgggcg tgctgctgtt tcttaccagc ccccggcaga tgttcgaaca gaaggccttc
     1321 tatggcttcc tatcgctgct ggtaaatatc ctgcgttcgg tgccgttcgt gatcctgttg
     1381 atcgtgatga tccccttcac ggtgatcgtc accggcacct cgctgggcgt cgccggggcc
     1441 atcccgccgc tggtagtcgg agccacgccg ttcttcgcgc gcctggtgga aaccgcgctg
     1501 cgggaagtcg accgcggcat tattgaagcc tcccaggcca tgggcgcaac cacccggcag
     1561 atcgtggtca acgcgctgct ccccgaggcg ttgcctggga tcatcgcagc catcaccgta
     1621 acggcgatca ccttggtgtc ctacaccgcc atgagcggcc tgattggcgg cggcggcctc
     1681 ggcgacctgg ccgtgcgtta cggctatcag cgttatcagc cggacgtgat ggcggtgacg
     1741 gtgatcctgc tgctggtgct ggtacagatt ctacaaaccg tcggcgacaa gctggtggtg
     1801 cacttttcca gaaaatagcg tgccccacgg ggcgcacaag acagcgggat accggttacc
     1861 tgccggcctc cgcacacatc ccacaaggag ctttacgatg aaaaaactga tcgctgcatt
     1921 cgctgccctg gcagccttct ccgcccaggc cgaaagcctg agcgtcgcgg ccactgccgt
     1981 accgcacgcg gaaatcctcg agttcgtt
//
LOCUS       DASRUM010000037         4458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1859020, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000037 DASRUM010000000
VERSION     DASRUM010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859020"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..695)
                     /locus_tag="VFF22_02800"
     CDS             complement(180..695)
                     /locus_tag="VFF22_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein M"
                     /protein_id="HZX15781.1"
                     /translation="MVSQRKGFTAQLDNSLLLQRWRALAPREQFSLGSLGAFLLLVLL
                     YLTLWQPTQERLAAARSAFETQRELNAYLHSQAPAARNVASTPQQAIDPERLQGMVTA
                     TAATQGLIIERLDNSGDGALQLNLQPAPFAQLLRWFTVLQEQGVQIAEAGLDRAEDNK
                     VAARLSLRVAQ"
     gene            complement(698..1834)
                     /gene="gspL"
                     /locus_tag="VFF22_02805"
     CDS             complement(698..1834)
                     /gene="gspL"
                     /locus_tag="VFF22_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HZX15782.1"
                     /translation="MSQTYVFLPPAACTGAQADLPVQWVADGVSETLEFGEAQTQLGA
                     SWTLVLPVEAVASCAVNLPTRKARWLRQALPFAVEELLAEEVELMHLALGEQLADGRH
                     RVFAVRRSWLAGWLALCPTAPQAIVVDADMLPGSGTALLPLHGRWLLGGENVTRMALQ
                     AQDWPQLSPLCAQPQVAWCDPQHPAPQPVDESIEVADPYVWLAQQLLRNNLAQGEFAV
                     QSSNGQWQRWRPLLGLVGMWLVLQWGFNLAQGWHLQRQADEYAAASAALYKELFPQDS
                     KLVNLRAQFDQHLQAGAGSGQTRLLGLLAQVSGALAAEGGQVQISQVDFSEVRGDLAM
                     QVQAAGFAELERLRERLQESGLAVQLGSASREGAGVSARVVIGG"
     gene            complement(1831..2796)
                     /gene="gspK"
                     /locus_tag="VFF22_02810"
     CDS             complement(1831..2796)
                     /gene="gspK"
                     /locus_tag="VFF22_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HZX15783.1"
                     /translation="MSRQRGMALITVLLVVAVVTVVSAGIIARQQLSIRSSANQLHVR
                     QAWHYAQGGETLVKAILQRDLREGDPRAPVDHPGEAWAKARAPFALDEGGALRVQITD
                     LTARFNLNSLLRQGQPNEAAVAQFRRLLLGLQIEAPYAERLLDWLDADDQPSGGYGAE
                     DNQYLLAQPPYRAGNRELKDVSELRLLLEMTEADYQRLSPYVSALPADATLNVNTASA
                     RVLASVAEGLPLSTAQGLVAARGSEGYRDLPSFTAQLSGLQVQSQGLAVGSQYFQVLS
                     EVTVGERRQVLRSTLQRASDGQLYVLARDLGQGGMPPAPVEEVEP"
     gene            complement(2793..3488)
                     /gene="gspJ"
                     /locus_tag="VFF22_02815"
     CDS             complement(2793..3488)
                     /gene="gspJ"
                     /locus_tag="VFF22_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012075071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HZX15784.1"
                     /translation="MRASRGFTLLELLIAIAIFALLGLGTYRMLDSVLQTDKVTRAHE
                     MQLRELVRAMAAFERDVLQVQARPTRDPFGDPRAALLGEDLDNPALELTRSGWRNPLG
                     QPRSGLQRVRWQLSGEQWQRHYWTVLDQAQDSQPQVQQALDGVTRLQLRYLDQEGTWQ
                     TSWPPLGNNPDDALKLLPQAVELVLEHRRYGELRRVLRLPEGLPERLQQAPEPSPDDP
                     AEPGGDIPEEPRS"
     gene            complement(3485..3874)
                     /gene="gspI"
                     /locus_tag="VFF22_02820"
     CDS             complement(3485..3874)
                     /gene="gspI"
                     /locus_tag="VFF22_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HZX15785.1"
                     /translation="MRRAGGFTLLEVLVALAIFALVAASVLTATARSLQTASRLEEKT
                     LAMWIADNQLAELQLSKTPVADGRDQGDVEFAGRRWQWQSEVLATSEADMRRVTLWVA
                     PAEQGRSGGDVRERAVVSLNGFLGVLQ"
     gene            complement(3871..4413)
                     /gene="gspH"
                     /locus_tag="VFF22_02825"
     CDS             complement(3871..4413)
                     /gene="gspH"
                     /locus_tag="VFF22_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HZX15786.1"
                     /translation="MRQQPSQAGGFTLIELLVVLVILGSLIGIAVLSVGIAGPGRELH
                     NEAERLAGLIGVLAEEAVLDNQEYGLLLSREAYQVLRYDSAKQSWQALSGKPHKLPGW
                     AELSVELEGAALELPLPEDEEPSKGALTPQLLLLSSGEISPFRLRLAERRADGLRLQL
                     VSDGFQLPKVESEQPAGRKR"
BASE COUNT          877 a         1552 c         1280 g          749 t
ORIGIN      
        1 accgccgacc ctctgcttgt aaggcagatg ctctcccagc tgagctaatc acccgctgct
       61 tgcgaggcgc gaaatttacg caagggccgc acctaagtca acaccctgct tcaagttttt
      121 ctaaaaaagt gcaaaaagtg ctgccctccc caggtgccac taagggactc gtcgcaccat
      181 tattgagcca cccgcagact tagccgagca gcaaccttat tgtcttcggc gcgatccagc
      241 ccggcttcgg cgatttgcac gccctgctcc tgcagcacag tgaaccaacg cagcaactgg
      301 gcaaaaggtg ccggctgcag gttgagctgc aaagcgccgt cgccggaatt gtccagacgc
      361 tcgatgatca gaccctgggt cgcagccgtg gccgtgacca tgccttgcag ccgctccgga
      421 tcaatcgcct gctgcggggt gctggccaca ttgcgcgccg ccggcgcctg gctatgcaga
      481 taggcattca actcgcgctg tgtttcaaat gcactgcgtg ctgcggccag gcgctcctgc
      541 gtcggttgcc agagcgtcag ataaagcagc accagcagca aaaacgcccc aagactgccg
      601 agcgagaatt gctcacgcgg cgccagagca cgccagcgct gcagcaacag cgaattatcc
      661 aactgcgcgg taaagccctt gcgttgcgaa accatactca tcctccgatc accacgcgcg
      721 cactgacgcc tgcaccttca cggctggccg agcccagctg caccgccaag ccactttcct
      781 gcaagcgctc acgcagacgc tccagctcgg caaaaccggc ggcctgcacc tgcatggcca
      841 agtcaccacg cacctcactg aaatccacct gactgatctg cacctggccg ccttcggcag
      901 ccagcgcgcc gctgacctgg gcgagcaagc cgagcaaacg ggtttgccca ctgcccgcgc
      961 cggcctgcag atgctgatca aactgcgcac gcaggttgac cagcttgctg tcctgcggga
     1021 acagctcctt gtacaacgcg gcgctggcgg cggcgtattc atcggcctga cgctgcaggt
     1081 gccagccctg cgccagattg aagccccact gcaacaccag ccacatgccg accagcccca
     1141 gcaatggccg ccagcgctgc cattggccgt tactgctttg cacggcgaac tcaccctggg
     1201 ccaggttatt acgcagcagc tgctgagcca gccaaacata gggatcggcc acctcaatgc
     1261 tttcatccac cggttgcggc gccgggtgct gcggatcaca ccaggcaact tgtggctgcg
     1321 cacacagggg gctcagctgc ggccagtcct gcgcctgcag cgccatccgc gtgacattct
     1381 cgccgcccaa caaccagcga ccgtgcagcg gcaagagcgc tgtacccgat ccaggcagca
     1441 tatcggcatc cacaacaatc gcctgcggcg ctgtggggca caacgccagc cagccggcca
     1501 gccagctgcg gcgcacggca aatacccgat gacggccatc ggccaattgc tcgcccaacg
     1561 ccagatgcat caactcgact tcttcggcga gcagctcttc caccgcaaac ggcaacgcct
     1621 gacgcagcca gcgggcttta cgcgtcggca gattgaccgc gcaggaggct accgcctcaa
     1681 cgggcaggac cagggtccag ctggcgccaa gctgcgtctg cgcctctccg aattccaggg
     1741 tttcgcttac cccgtcggcc acccactgca cgggtagatc cgcctgcgcg ccggtacaag
     1801 cggcgggtgg cagaaataca taggtctgac tcatggttcg acttcctcaa cgggcgccgg
     1861 cggcataccg ccctgcccca aatcacgggc tagaacatac agctgaccat cactggcgcg
     1921 ctgcaaggtg ctgcgtaaaa cctgacgacg ctcaccaaca gtgacttcgc tgagcacctg
     1981 aaaatactga ctgcctaccg ccaggccctg gctctgcacc tgcaagccgc tgagctgcgc
     2041 ggtaaagctc ggtaaatcac gatagccctc actgccccgc gcggcgacca gcccctgggc
     2101 ggtgctcagc ggcaatcctt cagccacgct ggccagcacc cgcgcgctgg cggtattgac
     2161 gttaagcgtg gcatccgccg gcaaggcgct gacatagggc gacaggcgct ggtagtccgc
     2221 ctcggtcatt tccagcaaca ggcgcagctc ggagacatcc ttcaactctc ggttgccagc
     2281 gcgataaggc ggctgcgcca gcaggtattg attgtcttcc gcaccgtaac cgccgctggg
     2341 ctgatcatcg gcatccaacc agtcgagcag gcgttcagcg taaggggctt cgatctgcag
     2401 ccccagcagc agacgacgaa attgcgccac ggcggcctcg ttgggttggc cctggcgcag
     2461 caagctgttg aggttgaaac gtgcagtcag atcggtgatc tgcacacgta aggcgccacc
     2521 ctcatccaga gcaaagggcg cacgggcttt ggcccaggcc tcgcccggat gatccaccgg
     2581 cgcacgcggg tcaccttcgc gcagatcgcg ctgcagaata gccttgacca gggtctcgcc
     2641 gccctgggcg taatgccagg cctggcgcac atgcagctga ttggcgctgc tacggatcga
     2701 cagctgctgg cgggcgataa tccccgcgct gaccacagtc accaccgcaa ctaccagcaa
     2761 gacggtgatc agcgccatgc cgcgctgccg actcatgagc gcggctcctc gggaatatcg
     2821 ccaccgggct cggcggggtc gtccgggctc ggctcgggtg cttgctgcaa gcgctccggc
     2881 aagccttctg gcaggcgcag cacacggcgc agctcgccat aacggcgatg ttccagcacc
     2941 agttcaacgg cctgcggcaa cagcttgagc gcatcgtccg ggttattgcc cagcggcggc
     3001 caactggtct gccaggtgcc ttcctgatcc agatagcgca attgcagtcg ggtcacccca
     3061 tccagcgctt gctgcacttg cggctggctg tcctgcgctt gatccagcac cgtccagtag
     3121 tgacgttgcc actgctcgcc actgagctgc cagcgcactc gctgcaggcc ggagcgcggc
     3181 tgcccaagag gattgcgcca gccgctgcgg gtcagctcca gggctgggtt atcgaggtct
     3241 tcaccgagca aggcggcgcg tggatcacca aagggatcgc gcgtgggcct ggcctgcacc
     3301 tgcaacacat cgcgctcgaa tgcggccatg gcgcgcacca gctcacgcaa ttgcatttca
     3361 tgggcacgag tgaccttatc ggtttgcagc acactgtcga gcatgcggta ggtgcccagc
     3421 cccaacagag caaagatggc gatggcgatc agcaattcga gcagggtaaa accgcgcgac
     3481 gccctcattg cagcaccccg agaaaaccat tcaggctgac caccgcgcgc tcacgtacat
     3541 cgccgcccga acgcccctgt tctgcgggcg cgacccacag ggtaacgcgg cgcatatccg
     3601 cctcgctggt ggccagtacc tcgctttgcc actgccagcg ccgcccagcg aactccacat
     3661 cgccctggtc gcgaccatcg gcgaccggag tcttcgacaa ctgcaactca gccagctggt
     3721 tgtcggcgat ccacatggcc agggtctttt cttccaaccg cgaggcggtt tgcaggctgc
     3781 gcgcggtggc ggtcagcacg ctggcggcga ccagcgcgaa aattgccaaa gcaaccagca
     3841 cctcaagcag ggtaaaaccg cccgcgcgcc tcatcgcttg cgccctgccg gctgctcgct
     3901 ctcgaccttg ggcagctgaa aaccatcgct aaccaattgc aggcgtaaac catcggcgcg
     3961 gcgctcggcc aagcgcaaac gaaacgggct gatttccccg ctggacagca acaacaactg
     4021 cggcgtcaga gcgcctttgc ttggctcttc gtcctcagga agaggcagct ccagggctgc
     4081 gccttccagc tcgacgctca gctcagccca gcccggcaat ttatggggtt tgcccgatag
     4141 cgcctgccaa ctctgtttcg ccgagtcgta gcgcagcacc tgataggcct cgcgactgag
     4201 cagcaggccg tactcctggt tatccagcac cgcttcctcg gccagtacac caatcaatcc
     4261 ggccaaccgt tcggcttcgt tatgcaactc acgcccagga ccggcgatgc ccacactgag
     4321 caccgcaata ccgatcaggc tgccaagaat caccagcacc accagcagtt cgatcagggt
     4381 aaagccccca gcctggctcg gttgctggcg catggggatc agaggtccca gttaccgata
     4441 tcggtatcct gatcgctg
//
LOCUS       DASRUM010000038        14984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1024198, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000038 DASRUM010000000
VERSION     DASRUM010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 14984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14984
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024198"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1991
                     /gene="dauA"
                     /locus_tag="VFF22_02830"
     CDS             252..1991
                     /gene="dauA"
                     /locus_tag="VFF22_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HZX15787.1"
                     /translation="MSLPLPPLFSAWRQTLRAGYGWKNLRSDLSAGVTVGIIAIPLAM
                     ALAIAVGVAPQHGLYTVLVAGTLIALFGGSRFNVSGPTAAFVVILLPVTQQFGLGGLL
                     LCTLMAGLILIALGLMRAGRLIEFIPYPVTLGFTAGIGIVIATLQIKDLFGLQLSSQP
                     NNYIEQLNGLLHALPSLQLGDSLVALVCLATLLIWPRWVPKVPGHLVALTVGALLGLL
                     LESLQIPVATIGERFSYNLDGVEHPGIPPFLPSFIWPWHLPGADGQPLEVSFELFRQL
                     LAPAFAIAMLGAIESLLCAVVADGMTGSKHDPNGELIGQGIGNLVAPLFGGITATAAI
                     ARSAANVRAGAYSPLAAIAHAGVVLIAILFLAPLFSYLPMAALAALLLVVAWNMSEPR
                     HVRHTLRIAPRSDVLVLLTCLILTVLFDMVLAVGVGLLLAAGLFIKRMSDLTDTEALP
                     KHFHQALNDLPEHVLAYAIRGPLFFGAAEKALSVLRRFTPGVKVVIVDISAVPLLDMT
                     AIAALDNVLRDYREQHVALVLSGPTPQVRLQLRRAGIVRVEGELAYVRDLAQAREKAL
                     RWLAPMADGESAT"
     gene            complement(2072..2629)
                     /locus_tag="VFF22_02835"
     CDS             complement(2072..2629)
                     /locus_tag="VFF22_02835"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HZX15788.1"
                     /translation="MSVDLAHIRQVMAEADCLHSNAEVEVAIDAMAAAINNEMADSNP
                     VVFCVMNGGLIFSGKLLPKLNFPLELSYLHATRYRNETSGGELFWKAKPEISFIDRDV
                     LIIDDILDEGHTLGAIIDFCRHAGAKNVRIAVLVDKTHDRKARPDLKADYVGMSCVDR
                     YVFGYGMDYKGYWRNAPGIYAVKGL"
     gene            2875..3513
                     /gene="upp"
                     /locus_tag="VFF22_02840"
     CDS             2875..3513
                     /gene="upp"
                     /locus_tag="VFF22_02840"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HZX15789.1"
                     /translation="MPILEIRHPLIRHKLGLMRRADISTKNFRELAQEVGALLTYEAT
                     KDLPLENCEIEGWCGTVQVEKISGKKITVVPILRAGIGMLEGVLSLIPGAKVSAVGVA
                     RNEETLQAHTYLEKLVPEIDERLAMIIDPMLATGGSMVATIDLLKKAGCKDIRAMVLV
                     AAPEGIKVVNDAHPDVLIFTASIDERLNEHGYIIPGLGDAGDKIFGTKQKDA"
     gene            3516..4796
                     /locus_tag="VFF22_02845"
     CDS             3516..4796
                     /locus_tag="VFF22_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HZX15790.1"
                     /translation="MQDHMQDEYNDPLWRQGISGAQMLFVAFGALVLMPLITGMDPNV
                     ALFTAGIGTLLFQLVTGRQVPVFLASSFAFIAPILAAKGEFGLPAVLGGIVASGLVYI
                     LLSAVVRVKGAGFIDRLLPPVVIAPVIISIGLALSPVAVNMAMGKAGDGSAQLVPYET
                     AMLISMSALVTTVLVAAMGRGLLRLVPILAGIIVGCIVAAFCGVIDTSNVAAAPWLAI
                     PAFVTPELHWGAILYIVPVALAPAIEHIGGVVAIGSVTGKNFIKKPGLHRTLLGDGLA
                     TSAAGLFGGPPNTTYAEVTGAVMLTKNYNPKIMTWAACFAIGLAFVGKFGTALQSIPV
                     PVMGGILCLLFGSIAVVGLNTLIRHQVDLSEARNLIIVSVTLVFGIGGMVIGNSDFAL
                     SGISLCAICALVLNLVLPGGQGWRSKTVLEEPDL"
     gene            complement(5048..6379)
                     /locus_tag="VFF22_02850"
     CDS             complement(5048..6379)
                     /locus_tag="VFF22_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX15791.1"
                     /translation="MPNNNNGYPSATRAWVTVAILMVAYVLSFIDRQILNLLVGPIRR
                     DLMISDTQMSLLMGLSFALFYTVCGIPLGRLADTKSRRGLIAIGVLFWSAATAACGMA
                     KLYWQFLICRIGVGVGEAALSPAAYSLIADSFPAERRATAISVYSMGVYLGSGLAFLL
                     GGLVIQFASAQGDVVLPVLGEVRPWQLIFLILGAAGVLFTLLMLAVKEPARRGVGAGI
                     AVPLADVGRYIRANRRTVLCHNFGFAGLAFAGYGSAAWIPTFFIRTYGWDASQVGIIY
                     GSIVAVFGCLGIVFGGRLADWMAKRGSSDANMRVGLYAAIGAVPFVLAFPLMETALWA
                     AVLTAPAVFFLSMPFGVAPAAIQEIMPNSMRGQASAIYLFVITLIGLGIGPTAVALVT
                     DYVFADDMALRYSLLIVTSLAVFSSIVLLSMSLKPYRESVVRLQGWSANPA"
     gene            complement(6536..7558)
                     /gene="hemH"
                     /locus_tag="VFF22_02855"
     CDS             complement(6536..7558)
                     /gene="hemH"
                     /locus_tag="VFF22_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HZX15792.1"
                     /translation="MTDHALLLVNLGSPASTDVADVRRYLNQFLMDPYVVDLPWPLRR
                     LLVSLILIKRPAASAHAYASIWWDEGSPLVVLSKRLHQAMKASWTHGPVELAMRYGEP
                     SLQTVLTRLAAQGIKQVTLAPLYPQFADSTTTTVIEEARRVVREQQLPMRFSILPPFY
                     DQPEYLDALVDSAKPYLEQDFDHLLLSFHGLPERHLHKLDPSNHCLKGADCCRTAAPE
                     VLATCYRAQCLRSAELFAQRMGLKPEQWSVSFQSRLGRAKWIEPYTEARLDELAKQGV
                     KKLLVMCPAFVADCIETLEEIGDRGREQFVEAGGESLQLVPCLNDHPSWVAALKTLSE
                     RAPLML"
     gene            complement(7567..8472)
                     /locus_tag="VFF22_02860"
     CDS             complement(7567..8472)
                     /locus_tag="VFF22_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HZX15793.1"
                     /translation="MHILLTGGTGLIGRALCRHWLQQGHQLTVWSRRPEQVATLCGEA
                     VRGIGRLDELGEEPLDAVVNLAGAPIADRPWTRKRKALLWASRIGLTEQLLAWLESRE
                     HKPKVLLSGSAVGWYGDGGERELHEDSPPVSEDFAAQLCGAWEETAQRAEDLGIRVVL
                     LRTGLVLASDGGLLKRLLLPFKLGLGGPLGNGRQWMPWIHLSDQIALIDFLLQQEQAS
                     GPYNACAPTPERNRAFSQALGRELHRPAFMPAPTFVLRVLLGELSVLLLGGQRAMPTR
                     LQEAGFSFRFTHLDVALADLLGQPD"
     gene            8677..9669
                     /locus_tag="VFF22_02865"
     CDS             8677..9669
                     /locus_tag="VFF22_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZX15794.1"
                     /translation="MIALHPIAIIGTGIAGLSAAQALHAAGHPVQLFDKSRGSGGRMA
                     SKRSDAGALDLGAQYFTARDRRFVEVVQQWQARGWVAEWTPSLYNFNNGQLSASPDEQ
                     VRWVGSPRMSAITRAMLGALPVNFACRITEVFRGEQHWHLQDAEGNNHGPFSHVIVAT
                     PAPQATALLSSVPKLAGAAASVAMDATWAVALAFANPLDTRVEGCFVQDSPLDWLARN
                     RSKPGRDTSLDTWVLHATSQWTKQHLDMPKEAVIEHLLGAFAELIGCAVPAPAFSLAH
                     RWLYARPASAHQWGVLADADLGIYACGDWCLSGRVEGAWLSGQEAARRLLEHLQ"
     gene            9666..9908
                     /locus_tag="VFF22_02870"
     CDS             9666..9908
                     /locus_tag="VFF22_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02450 family Trp-rich protein"
                     /protein_id="HZX15795.1"
                     /translation="MSVQARAPAKHRLNPRKLLLSKWTAAQPQNREKHFLVTELFCDE
                     DGTVLQIELQAVLNQRCQRLDWRALENPEHWRMGWK"
     gene            10034..10999
                     /locus_tag="VFF22_02875"
     CDS             10034..10999
                     /locus_tag="VFF22_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017476000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-thiouracil desulfurase family protein"
                     /protein_id="HZX15796.1"
                     /translation="MSPSETATGKPKLGISACLLGSEVRYNGGHKESRLCSRTLSEYF
                     DFAPLCPEVAIGMGIPREPIRLVGDPLAPHAVGTVNRTVDVTAPLAAYGERMAGELTD
                     ICGYIFMQQSPSCGLHRVKVYQDNGRPSEPGRGIFAQAFCARHPNLPVEEDGRLNDPI
                     LRENFITRVFAYSEWQQLLRDGLTRKRLIDFHSRYKYLLMATNPMQYKLLGRMLGDLG
                     QHDLNELAPRYFSELMSALKSCATRRTHSNVLQHLSGYLKQSLSSEEKQEMQQLIGQY
                     RHGVVPLVVPMTLLKHHFRRHPDTYIAQQVYLQPHPENLSLRNAL"
     gene            10996..11937
                     /locus_tag="VFF22_02880"
     CDS             10996..11937
                     /locus_tag="VFF22_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017904336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZX15797.1"
                     /translation="MSLEPITPGAAADDYQAAIAQGFMPIRDVARITGVNAVTLRAWE
                     RRYGLIVPHRTPKGHRLYSNEHVAHIQAILTWLNRGVSVSQVKGLLRSNQPAFAETSS
                     QWETKRQQLQEAICNLNERRLDECFNSELALYPPHTLCQQLLLPLLEDLELRWRNQFG
                     AQAERVFFYSWLRSKLGARLYHNNRQCNGAPLLLINVSDLPMAPGLWLTAWLASSAGC
                     PVEVFDWPLPPTELALAVEHIQPRAVLLYSSQALNSTHLQRLLGGYDCPCLLVGQVVQ
                     IHAEELVAITAQNAELSLAADPLAALHSLTDLNLLHS"
     gene            11952..13391
                     /gene="phrB"
                     /locus_tag="VFF22_02885"
     CDS             11952..13391
                     /gene="phrB"
                     /locus_tag="VFF22_02885"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HZX15798.1"
                     /translation="MQLMWFRTDLRVQDNSALTAAMSSGPTVALYLLSPGQWLSHDDA
                     PSKVDFWLRNLKELATELGKLNVPLLVREADHWSAAPQVIGELCQQLQIGTVQVNEEY
                     GVHESRRDQAVAEALDGLGVNFRSHLDQLFFKPGSVLTKSGGYFQVYSQFRKVCYQRL
                     HSALPTLIATPKAQAPQAIQSDAIPEQVAGFALLSAELRELWPAGEAEAARRLDDFAA
                     QQIDFYQSARDFPAKPGTSQLSTYLAAGVISPRQCLHAALQANQGEFDSGNVGVITWI
                     NELLWREFYKHILVGYPRVSMHRAFRVETEAVAWRDAPQELKAWQQGRTGFPIIDAAM
                     RQLLATGWMHNRLRMIVAMFLTKNLLIDWREGERFFMRHLIDGDLAANNGGWQWSSST
                     GTDSAPYFRIFNPLSQSQKFDPDGRFISQWLPELVGLNKTNIHNPAAIGGLFGIADYP
                     APIVDLSKSRTRALAAFKSLPHLAGEEVL"
     gene            13388..13810
                     /locus_tag="VFF22_02890"
     CDS             13388..13810
                     /locus_tag="VFF22_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZX15799.1"
                     /translation="MSDFLRQFAQDFAGLNKDNLDRLGQLYSDDALFCDPLHEVRGLA
                     NMQRYFGEMYANVSELRFDFYGFDQVRDGEGYLRWTMSYRHPRLAGGQLIRVEGCSHL
                     LWQDKVYRHRDYFDAGSLLYEHLPLLGSAIRWLKRRLA"
     gene            13807..14586
                     /locus_tag="VFF22_02895"
     CDS             13807..14586
                     /locus_tag="VFF22_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015636967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZX15800.1"
                     /translation="MNANARRIWLTGASSGIGAALTEQLLEQGHRLAISARSIEPLQA
                     LAKRYPQQVLVVAGDLGDAAQVRAIGEHIVQVWGALDCVILNAGTCEYVEVSAFEADM
                     VERVMRANLLSASYCIEAALPLLRLGNRPHLVGVGSSVTFMPLPRAEAYGASKAAMRY
                     LLEALRIDLHAENIAVTLVSPGFVDTPLTQKNDFPMPMRWPVDKAARYIAERLHQQPF
                     EISFPGPFIAMLKLLGHLPKRLQVAIGARMARTEAKTKDNP"
     gene            14583..>14984
                     /locus_tag="VFF22_02900"
     CDS             14583..>14984
                     /locus_tag="VFF22_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010428680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZX15801.1"
                     /translation="MKIAIIGSGIAGLTSAYLLNRSHDITVFEAGDWIGGHTHTVDVQ
                     VAGQSYAVDTGFIVFNDWTYPNFIRLLGQLGVGFKATEMSFSVSDPVSGVEYNGHSLN
                     SLFAQRSNLLSPKFFGMLRDILRFNREALDDL"
BASE COUNT         2927 a         4864 c         4450 g         2743 t
ORIGIN      
        1 cttaggaaca gcttgatcgg ctcgtcacca atctcttcgc caatcacctg tttcttgtct
       61 ttacgcatca ctcactccag ctaaccaggc cacacggggg cggcagtgta cctccggggc
      121 gggcaagggc tcaacggatg cgcggcaacc gtaccgtgta acacgcgctg aggctggcgc
      181 acagccgact taggtcaaca ctgcacgctg gggccgcccc ttatactgca cttatcgagt
      241 tgggagtcat tatgtcgctg cccttaccgc cgctgttctc cgcctggcgc cagaccttac
      301 gcgccggtta cggctggaaa aacctgcgca gcgacctgag cgccggcgtt acggtgggca
      361 tcatcgctat tccgctggcc atggccctgg cgattgcagt cggtgtggcg ccgcaacacg
      421 gcctttatac ggtgctggta gccggcacgc tgatcgccct gtttggcggt tcgcgcttca
      481 atgtttccgg gccgaccgca gcctttgtgg taattctgct gccagtcacc cagcaattcg
      541 gcctcggcgg tctgctgctg tgcaccctga tggccggact gatcctgatt gccctcgggc
      601 tgatgcgcgc cgggcggcta atcgaattta tcccctaccc ggtcacatta ggctttaccg
      661 ccggtattgg catcgtcatc gctacgctgc agatcaagga tctgtttggc ctgcaactca
      721 gcagccagcc gaacaactac atcgaacaac tcaacggcct gctgcatgcc ctgcccagcc
      781 tgcaactggg tgacagcctg gtagcgctgg tgtgcctggc aactttgctg atctggccgc
      841 gctgggtgcc gaaagtaccg gggcatctgg tggcgctgac ggtcggcgcc ttgctcgggc
      901 tgctgctgga aagcctgcag attcccgtgg caaccattgg cgaacgcttc agctacaacc
      961 tggatggcgt tgaacacccc ggtattccgc cgtttctgcc gagttttatc tggccctggc
     1021 acttacctgg cgccgatggc caaccgctgg aggtgagctt cgagctgttc cgtcagctgc
     1081 tcgcaccggc ctttgccatc gccatgctcg gggcgattga gtcgctgctg tgcgcggtgg
     1141 tcgccgatgg catgaccggc agcaaacacg accccaatgg cgagctgatc ggccagggca
     1201 tcggtaatct ggttgcgccg ctgttcggcg gcatcaccgc caccgccgcg attgcccgca
     1261 gcgccgccaa cgtgcgcgcc ggggcttact cgccgctggc cgcgattgct cacgccggcg
     1321 tggtgctgat cgccatcctg tttctcgcgc ccctgttcag ttacttgccg atggcggcgc
     1381 tggcggcctt gctgctggtg gtggcgtgga acatgagcga gccgcggcat gtgcgccaca
     1441 ccctgcgcat agctccgcgc agtgatgtac tggtgctgct gacttgcctg attctcaccg
     1501 ttctgttcga tatggtgctg gccgtgggcg tcggcctgct gctggccgcc ggcctgttta
     1561 tcaagcgcat gagcgacctt accgataccg aagcgctgcc caagcacttt caccaggcac
     1621 tgaatgacct gcccgaacac gtgctggcct acgccattcg cggcccgctg ttcttcggcg
     1681 cggcggaaaa agccctgagc gtgctcaggc gctttactcc aggagtgaag gtggtgatcg
     1741 tcgatatcag cgccgtgccg ctgctggata tgaccgccat cgccgccctg gacaacgtgc
     1801 tgcgcgatta ccgcgagcag cacgtcgctc tggtgctgag cggcccgacg ccgcaggtac
     1861 gcctgcaatt gcgccgcgcc ggcatcgtgc gcgtagaggg tgagctggcc tatgtgcgtg
     1921 acctggccca ggcccgggaa aaggcgctgc gctggttggc gcccatggcc gatggggaat
     1981 cagcaacgta gggtggataa cgcgcagctt atccaccatg caccttgtag tggagaacgc
     2041 ttcgcggttt tccaccctac aaaggcggtg cttacagccc tttaaccgcg taaatacccg
     2101 gcgcattgcg ccagtagccc ttgtaatcca tgccgtagcc aaacacgtag cgatcaacgc
     2161 aggacatgcc gacgtaatca gccttgaggt cggggcgcgc cttgcggtcg tgggtcttgt
     2221 ctaccagcac ggcaatgcgc acgttcttgg caccggcatg ccggcaaaaa tcgatgattg
     2281 caccgagggt gtgcccttca tcgaggatgt cgtcgatgat cagcacgtcg cggtcaataa
     2341 aggagatttc cggcttggcc ttccagaaca gctcgccgcc gctggtttca ttgcggtagc
     2401 gggtggcgtg caggtaggac aattccagcg ggaagttgag cttgggtagc aacttgccgg
     2461 agaagatcag cccgccgttc atcacgcaga acaccacggg attgctgtcg gccatctcgt
     2521 tgttgatggc agccgccatg gcgtcgatag ccacctcgac ctcggcattg ctgtgcaggc
     2581 aatcggcttc ggccataact tggcggatgt gcgcgagatc gacggacatg ctgtttcctc
     2641 ttgggagcgg gacgaccaga ccagaaagct gatcaatgcg tcaaaataaa agccggcaaa
     2701 ggtacccatc tgcgccaggc agagcaaggg ttgccagaca aacagggcaa tttgtcggca
     2761 acaggcgaaa tttagacgct aattgccgat ttcacccaag cccgaggcag cacttggact
     2821 agccaatgct ttaatctaac gcaatttttc gcccgtcctg ccggagaccc cgccatgcct
     2881 atcctcgaga tccgccaccc gctgatccgc cacaaactcg gcctgatgcg ccgcgccgat
     2941 atcagcacga agaattttcg tgagctggcc caggaagtcg gcgcactgct gacctacgag
     3001 gcgaccaaag atctgccgct ggaaaactgc gagatcgaag gctggtgcgg caccgtgcag
     3061 gtggaaaaaa tctccggcaa gaagatcacc gtagtgccaa tcctccgtgc cggcatcggc
     3121 atgctcgaag gcgtgctcag cctgattccg ggggccaagg tcagcgccgt gggcgtggcg
     3181 cgcaacgaag aaaccctgca agcgcacacc tatctggaaa aactggtgcc ggaaatcgac
     3241 gagcgcctgg cgatgatcat cgacccgatg ctcgccactg gtggctcgat ggtcgccacc
     3301 atcgacctac tgaagaaagc cggctgcaag gacatccgcg ccatggtgct ggtcgccgcc
     3361 cccgaaggca tcaaagtggt caacgacgcg cacccggatg tgctgatttt caccgcctcc
     3421 atcgacgagc gcctgaacga gcacggctac atcattcccg gcctcggtga tgccggcgac
     3481 aagatcttcg gcaccaagca gaaggacgcc tgaccatgca ggatcatatg caggacgaat
     3541 acaacgaccc actctggcgc cagggcattt ccggcgcgca gatgctattt gtggccttcg
     3601 gcgccctggt gctgatgccg ctgatcaccg gcatggaccc caacgtcgcg ctgtttaccg
     3661 ccggcatcgg caccctgcta ttccaactgg taaccggccg ccaggtaccg gtattcctgg
     3721 cctccagctt tgcctttatc gcgccgattc tcgccgcaaa aggtgagttc ggcctgccgg
     3781 ccgtgctcgg cggcatcgtc gcctcgggcc tggtgtatat cctgctcagc gccgtggtgc
     3841 gggttaaagg cgcgggcttt atcgaccgtc tgttgccacc ggtggtgatt gctccggtga
     3901 tcatttccat cggcctggcg ctgtcgcccg tggcggtgaa tatggccatg ggcaaggccg
     3961 gcgatggcag cgcgcagctg gtgccgtatg aaaccgccat gctgatttcc atgtcggcgc
     4021 tggtcactac cgtgctggta gcggccatgg gtcgtggctt gctgcgtctg gtgccgatcc
     4081 tcgcggggat catcgtcggc tgcatcgtcg cggcgttctg tggggtgatc gacaccagta
     4141 atgttgcggc cgcaccttgg ctggcgattc cggcgttcgt aacacctgag ctgcactggg
     4201 gtgcgattct ctacatcgtt ccggtcgctt tggcgccggc tattgagcac attggtggcg
     4261 tggtagcgat cggcagcgtg accggcaaaa actttatcaa gaagcccggc ctgcaccgca
     4321 ccctgctcgg cgacggcctg gccacctcgg ccgcaggcct gttcggcggc ccaccgaaca
     4381 ccacctatgc agaagttact ggcgcggtaa tgctgaccaa gaactacaac ccgaagatca
     4441 tgacctgggc ggcctgcttc gccatcggtc tggcctttgt cggcaagttc ggcaccgcgc
     4501 tgcagagcat tccggtaccg gtgatgggcg gcattctctg cctgctgttc ggctccatcg
     4561 cggtggtggg cctgaacacc ctgatccgcc atcaggtcga cctgtccgag gcgcgcaacc
     4621 tgatcatcgt ttcggtgact ctggtgttcg gcatcggcgg tatggtgatt ggcaacagcg
     4681 acttcgccct ctccggcatt tccctgtgcg ccatctgcgc gctggtgctt aacctggtat
     4741 tgccgggtgg ccagggctgg cgcagcaaga ccgtgctgga agagccggac ctgtaaatcg
     4801 cgcccactaa aaagccccgc atctgcgggg cttttttatg ccgctaacta tctagagcct
     4861 gtttacgatc tcgcgagcta gagccatgca agacaaaaac cggcgaggaa gcggagtgta
     4921 cttttgtaca tgagcattac tcgcttcact cgcccttcga gtcgcgctaa agcgcgttgc
     4981 cgcaagcggc ttgccgagcc ggtttttaac gcagcagggc cgacgcgcag cagatcgtag
     5041 gcaggctcta agccgggttg gccgaccatc cctgcaaccg caccacactc tcgcgatacg
     5101 gtttgaggct catgctcagc agcacgatgg agctgaacac agccagcgag gtaacaatca
     5161 gcagcgaata acgcagcgcc atgtcatcgg caaacacgta gtcagtcacc agcgccaccg
     5221 ccgtcgggcc gatgcccagg ccgatcagag tgatcacgaa cagatagatc gccgacgcct
     5281 ggccgcgcat cgagttgggc atgatttcct gaatcgccgc cggtgccacg ccaaacggca
     5341 tgctcaggaa aaataccgcc ggcgcggtga ggactgccgc ccacagcgca gtttccatca
     5401 gcgggaaggc cagcacaaaa ggcaccgcac caatggccgc atacaggccg acacgcatat
     5461 tggcgtcgct gctgccgcgc ttggccatcc agtcagccag gcggccgccg aagacgatac
     5521 ccagacaacc gaagaccgca acaatgctgc cgtagataat gccgacctgg ctggcgtccc
     5581 agccgtaggt gcggataaag aaggtcggaa tccacgccgc gctgccgtaa ccggcaaagg
     5641 ccaggccggc aaagccgaag ttatggcaca acacggtgcg gcggttggcg cggatataac
     5701 gtccaacatc cgccagcggc accgctatac cggcgcctac accacgccgt gcaggctcct
     5761 tcaccgccag catcaacagg gtaaacagca ccccggccgc gccgagaatc aggaagatca
     5821 actgccaggg ccgcacttca ccgagcaccg gcagcaccac atcgccttgc gccgaggcga
     5881 actgaatcac cagaccgccg agcaggaagg ccaacccgga gccgaggtaa acccccatgg
     5941 aatacacgct gatcgcggta gcgcggcgct cggccggaaa gctgtcggca atcagcgaat
     6001 aggccgcagg cgacagcgcc gcctcgccga caccgacgcc gatgcggcag atcaaaaatt
     6061 gccagtacag cttggccatg ccgcaggcag cggtggccgc actccagaac agcacgccga
     6121 tggcaatcaa cccacggcgg ctcttggtgt cggccagtcg gcccagagga atgccgcaga
     6181 cggtatagaa cagcgcgaat gacaggccca tcaacaggct catctgcgtg tcgctaatca
     6241 tcaggtcgcg gcgaatcggc ccaaccagca gattgagaat ctgccggtcg ataaacgaca
     6301 gcacatacgc caccatcagg atggcgacag ttacccaggc gcgggtagca gagggatagc
     6361 cgttattgtt gttgggcact ggcagtctcc tcggcaccgc tggtgcggtg cacgacggtt
     6421 aaaagaacta ccagcttagg ccgacataca gggcgatgag tatcgcccta aggtgttatc
     6481 agtgaggcga atgcgcggaa tcccttcgag cgaatgacag ccgcgtccga ttaggctaga
     6541 gcatcagcgg agcacgctcg ctaagggtct tcagcgctgc cacccagctg ggatgatcat
     6601 tcaggcacgg caccaactgc aggctctcgc caccggcttc gacaaactgc tcacggccac
     6661 gatcaccaat ttcttccagg gtttcgatgc aatcggcgac aaaggccggg cacatcacca
     6721 gcaacttctt cacgccctgt ttggccagct cgtcgagacg cgcctcggtg tagggctcga
     6781 tccacttggc gcggcccagg cgcgactgga acgacaccga ccactgctca ggcttaagcc
     6841 ccatgcgctg agcgaacagc tcggcgctgc gcaggcactg ggcgcggtaa caggtagcga
     6901 gcacttcggg cgcggccgtg cgacagcaat cggcgccttt cagacagtga ttgctggggt
     6961 cgagcttgtg caggtgacgc tccggcaggc cgtggaaact cagcagcaga tggtcgaaat
     7021 cctgctccag ataaggcttg gcgctatcga ccagagcatc caggtattcc ggttggtcat
     7081 agaacggcgg caggatggaa aagcgcattg gcagttgttg ctcacgcacc acccgccgcg
     7141 cctcttcgat caccgtggtg gtggtgctgt cggcaaattg cgggtacagc ggcgccaggg
     7201 tgacctgctt gatcccctgg gcggccaggc gggtcagcac ggtttgcagc gacggctcgc
     7261 catagcgcat ggccagttcc accgggccgt gggtccagga cgccttcatc gcctgatgca
     7321 gacgcttgct cagcaccacc agcggcgagc cttcatccca ccagatcgag gcataggcgt
     7381 gcgccgaggc cgccgggcgc ttgatcagaa tcagcgaaac cagcagacgc cgcagcggcc
     7441 agggtagatc gacgacatag gggtccatca gaaactggtt gaggtaacgg cgcacatcgg
     7501 ccacgtcagt ggaagccggc gagcccagat ttaccaacag caaggcgtga tcggtcatgc
     7561 aaaatcctag tcaggttggc ccagcagatc cgccagagcc acatccaaat gagtaaaacg
     7621 aaaactgaaa ccagcctcct gcaagcgcgt cggcatcgcg cgctggccac cgagcagcag
     7681 caccgacagc tcacccagca gtacacgcag gacaaacgtc ggggccggca taaaggccgg
     7741 ccgatgcagc tcgcggccca gagcctggct gaaggcgcgg ttacgctcag gcgtcggcgc
     7801 gcaggcatta taaggaccgc tggcctgctc ctgctgcagg agaaaatcaa tcagggcgat
     7861 ttgatcgctg agatgtatcc acggcatcca ctgccgacca ttgcccagcg ggccgcccaa
     7921 cccgagcttg aacggcagca gcagacgctt gagcagcccg ccgtcacttg ccagcaccag
     7981 cccggtgcgc agcagcacca cacggatacc taaatcctcg gcgcgttggg cagtttcttc
     8041 ccaggcgcca cacagctgcg cggcaaaatc ctcacttact ggcggcgaat cctcatgcag
     8101 ctcgcgctcg ccaccatcgc cgtaccaacc cactgccgaa ccggataaca acaccttcgg
     8161 cttgtgctcg cggctctcca gccaggccag cagttgttcg gtcaggccaa tgcggctggc
     8221 ccacagcagc gccttgcgct tgcgcgtcca gggccggtcg gcaatcggcg cgccggccag
     8281 attgaccaca gcatccagcg gttcttcgcc cagctcatcg agccggccaa taccgcgcac
     8341 cgcttcacca cacagcgtcg cgacctgctc aggtctgcgg ctccacaccg ttaattggtg
     8401 accctgttgc aaccagtggc ggcacagcgc ccgcccgatt aaaccggttc cgcccgtcag
     8461 aagaatgtgc atcacgttgt cctcgcatgg cctttgcatg gctctgcata gttagtaact
     8521 accgacggat taataacttt tgtggcttca atccgttcgc aggttgtctt aactatagga
     8581 aggcaggatc gactccggca tatcctatac agaaaaatcg tattgtatag cttttgtcta
     8641 aagcgtagcc taacgcttga aaatacgagg tagaccatga tcgctctaca ccccatcgcc
     8701 atcatcggca ccggcatcgc cgggctgtct gccgcacagg cgctgcatgc cgccggacac
     8761 ccggtgcaac tgttcgacaa gagccgcggc agtggcggcc gcatggccag caaacgcagt
     8821 gatgccggcg cgctggacct tggcgcgcag tacttcaccg cccgcgaccg tcgctttgtc
     8881 gaggtggtgc agcagtggca agcccgtggc tgggtcgccg aatggacccc cagcctctac
     8941 aacttcaata acggccagct cagcgcctcg ccggacgagc aagtgcgctg ggtcggcagc
     9001 ccgcgcatga gtgcaatcac ccgcgccatg ctcggcgcct tgccggtgaa tttcgcctgc
     9061 cgcatcactg aagtgtttcg cggcgagcag cactggcacc tgcaggatgc ggaaggcaac
     9121 aaccacggcc cattcagcca tgtgatcgtc gccaccccgg cgccgcaggc cacggcactg
     9181 ctgtccagcg tgccgaaact cgccggcgct gcggcaagtg tggcgatgga cgcaacctgg
     9241 gcggttgctc tggcctttgc caacccgctg gatacccgcg tcgaaggctg ctttgtacag
     9301 gacagtccgc tcgactggct ggcgcgcaac cgcagcaaac ccggccgcga caccagcctc
     9361 gacacctggg tgctgcacgc caccagccag tggaccaagc agcacctgga tatgcccaaa
     9421 gaagcggtga tcgaacacct gctcggcgcc ttcgccgaac tgatcggttg cgccgtgccc
     9481 gccccagcct tcagcctggc gcaccgctgg ctctacgccc gcccggccag cgcgcaccag
     9541 tggggcgtac tggccgatgc cgacctgggc atctatgcct gtggcgactg gtgcctatcc
     9601 gggcgggtcg aaggcgcctg gctgagcggc caggaagcgg cgcgccgcct gcttgaacac
     9661 ctgcaatgag cgttcaagcc cgcgcaccag caaaacatcg acttaatccg cgcaagctgc
     9721 tgctgtcgaa atggacagca gcgcagccgc agaaccgcga gaagcatttt ctcgtcaccg
     9781 agttgttctg cgacgaagac ggcacggtgc tgcagatcga attgcaggcc gtgctcaacc
     9841 agcgctgcca gcgcctggac tggcgtgctc tggaaaaccc cgagcactgg cgtatgggct
     9901 ggaagtaaca ggcttacaca caaaacccta cattagttgt acaaagatat tgacttgtac
     9961 aattacaaac ctatgatgaa cgtaagttgt acatccatca ggatctgtac aagtcacgca
    10021 tcagaggttg cccatgagcc catccgaaac cgccaccggc aaacccaaac tgggcatcag
    10081 cgcctgccta ctgggttccg aggttcgcta caacggcggc cacaaggaat cgcgcctgtg
    10141 cagccgcacc ctcagcgaat atttcgactt cgccccgctg tgcccggaag tggccatcgg
    10201 catgggcatc ccgcgtgagc cgatccgcct ggtcggcgac ccgttggcgc cgcatgccgt
    10261 gggcaccgtc aaccgcacag tcgatgtcac tgcgccgctg gccgcctatg gcgagcgcat
    10321 ggccggtgag ttgacggata tctgcggcta tatctttatg cagcagtcgc cgtcctgtgg
    10381 cctgcaccgg gtcaaggtct atcaggacaa cggccggcca agcgagccgg ggcgcggcat
    10441 cttcgcccaa gcattctgcg cgcggcaccc caacctgccg gtggaagaag atggccgcct
    10501 caacgacccg atcctgcgcg agaacttcat cacccgcgtg ttcgcctatt cggagtggca
    10561 acagctgctg cgtgatggcc tgacgcgcaa gcgcctgatc gacttccact cgcgctacaa
    10621 atacctgctg atggccacca accccatgca gtacaaactg ctcgggcgca tgctcggtga
    10681 cctcggccag catgacctga atgagctggc gccacgttat ttcagtgaac tgatgagcgc
    10741 gctgaaaagc tgcgccaccc gccgcaccca cagcaacgtg ctgcagcacc tcagcggcta
    10801 cctcaagcaa tcactgagca gtgaagaaaa gcaggaaatg cagcaactga tcggccaata
    10861 ccgccatggc gtggtaccgc tggtcgtgcc catgaccctg ctcaagcacc acttccgccg
    10921 ccacccggac acctacatcg cacagcaggt ttacctgcaa ccgcacccgg aaaacctcag
    10981 cctgcgtaac gccctatgag ccttgagcca atcactccag gcgcagccgc cgacgactac
    11041 caggcggcca tcgcccaggg ttttatgccg atccgcgatg tggcgcgcat caccggggtc
    11101 aacgccgtga ccctgcgcgc ctgggaacgg cgttacggcc tgatcgtgcc gcaccgcacg
    11161 cccaagggtc atcgcctgta ttccaatgaa catgtcgcac acatccaggc aattctcacc
    11221 tggctcaatc gcggcgtttc agtcagccag gtcaaaggcc tgctgcgctc caatcagccg
    11281 gcgtttgccg aaaccagctc gcagtgggaa accaagcgcc agcagttgca ggaagccatc
    11341 tgcaacctca atgagcggcg tctggatgag tgttttaaca gcgagctggc gctctacccg
    11401 cctcataccc tgtgccagca actgctcctg ccgctgcttg aggatttgga attgcgctgg
    11461 cgcaaccagt tcggcgccca ggccgagcgg gtgttcttct attcctggct gcgcagcaag
    11521 cttggtgcgc gcctgtacca caacaaccgt cagtgcaacg gcgcgccgct gctgctgatc
    11581 aacgtttcgg acctgccgat ggcccccggt ctgtggctca ccgcctggct ggcgagtagc
    11641 gcaggctgcc cggtggaagt cttcgactgg ccgctgccgc cgaccgaact ggccctggcg
    11701 gtcgagcata ttcagccgcg tgcggtgctg ctgtattcca gccaggcgct gaacagcacc
    11761 cacctgcaac gtctgctcgg tggttatgac tgcccctgcc tgttggtcgg tcaggtggtg
    11821 cagatccacg ccgaagaatt agtcgccatc acagcgcaga acgccgaact aagcctggcc
    11881 gccgacccgc tggccgcgct gcacagcctg accgacctca acctgctgca cagctgatgg
    11941 gaaataccat gctgcaactc atgtggtttc gtaccgacct gcgcgtgcag gacaacagcg
    12001 ccctgaccgc agccatgagc agcggcccga ccgtggcgct gtacctgctc agccccggtc
    12061 agtggctgag ccacgatgat gcgccgagca aggtcgactt ctggctgcgc aacctcaaag
    12121 agctggccac tgaacttggc aaactcaatg tgccgttgct ggtacgggaa gccgatcact
    12181 ggagcgccgc gccccaggtg atcggcgaac tgtgccagca gctgcagatc ggtaccgtgc
    12241 aggtcaacga agagtacggc gtgcatgaaa gccgccgcga ccaggccgtg gccgaggcgc
    12301 ttgatggttt gggggtgaat tttcgcagcc atctggatca gctgttcttc aagcctggca
    12361 gcgtactgac caaatccggc ggctactttc aggtctatag ccagttccgc aaggtctgct
    12421 atcagcgcct gcatagcgca ttgcccacgc tgatcgccac gcctaaagcg caggcgccgc
    12481 aggcgatcca aagcgatgcc attcctgaac aggttgcagg cttcgccctg ctctctgccg
    12541 aattgcgtga gctgtggcca gccggtgaag ccgaggcggc gcggcgcctg gatgattttg
    12601 ccgcgcagca gatcgacttc taccagagcg cccgcgactt tccggccaag cccggcacca
    12661 gccagctctc cacctacctg gccgccggag tgatctcgcc gcgccagtgc ctgcatgcgg
    12721 ccttgcaggc caaccagggc gagttcgaca gcggcaatgt cggggtgatt acctggatca
    12781 acgagctgct ctggcgtgag ttctacaaac acatcctggt cggctacccg cgcgtctcca
    12841 tgcaccgcgc cttccgtgtg gaaaccgagg ccgtggcctg gcgtgatgca ccgcaagagc
    12901 tgaaagcctg gcagcaaggc cgcaccggtt tccctattat cgatgcagcc atgcgccagc
    12961 tgctggccac cggctggatg cacaaccgcc tgcgcatgat cgtggcgatg ttcctgacca
    13021 agaatctgct gatcgactgg cgcgaaggcg agcgcttctt tatgcgccac ctgatcgatg
    13081 gcgacctggc ggccaacaac ggcggctggc agtggagttc gtctaccggc accgattcgg
    13141 cgccctactt ccgcattttc aatccgctga gccagtcgca gaaattcgac ccagatggcc
    13201 gttttatcag ccagtggctg cccgagctgg tcggcctgaa caaaaccaat atccataacc
    13261 cggcggcgat cggcgggttg ttcggtatcg ccgattaccc cgcgccgatt gttgacctga
    13321 gcaagagccg cacgcgcgcc ctggcggcgt tcaagagcct gccgcacctg gccggcgagg
    13381 aggtgctgtg agcgactttc tgcgccaatt cgcccaggat tttgccgggc tgaacaaaga
    13441 caacctcgac cgcctgggcc agctctacag cgacgatgca ttgttctgcg acccactgca
    13501 tgaggtgcgt gggctggcca atatgcagcg ctacttcggc gagatgtacg ccaacgtcag
    13561 cgaactgcgc ttcgacttct acggctttga tcaggtgcgc gacggtgaag gctacctgcg
    13621 ctggaccatg agttaccgcc atccacgcct ggccggcgga cagctgatcc gcgtggaggg
    13681 ctgctcgcac ctgctgtggc aggacaaggt ctatcgccac cgcgactatt tcgacgccgg
    13741 cagcctgctc tatgagcacc tgcccttact cggctcggcg attcgctggc tgaaacggag
    13801 gttggcatga acgcaaacgc acggcgtatc tggctcactg gcgccagcag tggcatcggc
    13861 gcggcactga cggaacaact gctcgagcag ggtcaccgcc tggctatcag cgcccgcagc
    13921 attgagcctt tacaggcgct ggccaagcgt tatccgcagc aggtgctggt ggtagcgggc
    13981 gaccttggcg atgccgccca ggtgcgtgca atcggcgagc atatcgtcca ggtctggggt
    14041 gcgctggatt gcgtcatcct caacgccggc acctgtgaat atgtcgaggt cagcgccttc
    14101 gaggcggaca tggttgagcg agtcatgcgc gccaacctgc tctccgccag ttactgcatc
    14161 gaagccgccc tgcctttgct gcgcctgggc aaccggccgc acctagtcgg tgtcggcagc
    14221 tcggtaacct ttatgccgct gccgcgggct gaggcctacg gcgcctccaa agcagccatg
    14281 cgctacctgc tggaagccct gcgcattgac ctgcacgccg aaaacattgc cgtcaccctg
    14341 gtcagccccg gctttgtcga caccccgctg acccagaaga atgacttccc catgcccatg
    14401 cgttggccag tagacaaagc cgcccgctat atcgccgagc gcctgcacca gcagcccttt
    14461 gaaataagct tccccggccc ctttatcgcc atgctcaagc tgctcggcca tctgcccaag
    14521 cgcttgcagg tggccattgg cgcccgtatg gcccgcactg aagccaagac caaggacaac
    14581 ccatgaagat cgcaattatc ggcagcggta tcgccggttt gaccagcgcc tacctgctta
    14641 accgcagcca cgacatcact gtgttcgagg ctggcgactg gattggcggg cacacccaca
    14701 ccgtggatgt gcaggttgct ggccaaagct acgccgtcga caccggtttt atcgtgttca
    14761 acgactggac ctacccgaac tttatccgcc tgctgggcca gctcggcgtc ggcttcaagg
    14821 ccaccgagat gagcttctcg gtcagcgacc cggttagcgg cgtggagtac aacgggcaca
    14881 gcctcaacag cctgttcgcc cagcgcagca acctgctgtc gccgaagttc ttcggcatgc
    14941 tgcgcgacat cctgcgtttc aaccgcgagg cgctggacga cctg
//
LOCUS       DASRUM010000039        81884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2028779, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000039 DASRUM010000000
VERSION     DASRUM010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 81884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81884
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2028779"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..510)
                     /locus_tag="VFF22_02905"
     CDS             complement(145..510)
                     /locus_tag="VFF22_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5064 family protein"
                     /protein_id="HZX15802.1"
                     /translation="MFTPGHIHRSNLDLSEGLRLAPPFSIDFYYEVRQDPQEGPMLHM
                     RLVGEVQGRHFDEEFELHRDVAFNFASAATRVAAKHGLPPNASPIMRGHDEYDKVFAD
                     IRQQLHAKPGEPVNLDHID"
     gene            complement(557..1483)
                     /gene="arcC"
                     /locus_tag="VFF22_02910"
     CDS             complement(557..1483)
                     /gene="arcC"
                     /locus_tag="VFF22_02910"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HZX15803.1"
                     /translation="MRLVIALGGNALLRRGEAMTAENQRENVRIACEQIAKVAPGNEL
                     VIAHGNGPQVGLLALQGNAYDPSNPFPLDVLGAETEGMIGYMIEQELGNLLPFEVPFA
                     TILTQVEVDSGDPAFKKPSKPIGPVYSKEEAERLAAEKGWSIAPDGDKFRRVVASPRP
                     QRIFEIRPIKWLLEKGSVVICAGGGGIPTMYDGNQLRGVEAVIDKDLCSALLAEQLNS
                     DLLVIATDVDAAYIDWGKPTQTSIAEAHPDEMDKLGFAAGSMGPKVQAACEFVRNTGG
                     TAVIGSLENIEAIVQGTSGTRISLASDGIRFY"
     gene            complement(1557..2567)
                     /locus_tag="VFF22_02915"
     CDS             complement(1557..2567)
                     /locus_tag="VFF22_02915"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZX15804.1"
                     /translation="MAFNMHNRNLLSLMHHSARELRYLLDLARDLKRAKYTGTEQQHL
                     QRKNIALIFEKTSTRTRCAFEVAAYDQGANVTYIDPNSSQIGHKESMKDTARVLGRMY
                     DAIEYRGFKQEIVEDLAQYAGVPVFNGLTDEYHPTQMLADVMTMREHSDKALHEISYA
                     YLGDARNNMGNSLLLIGAKLGMDVRIAAPKALWPSAEHIAACQHFAEESGARISLTED
                     PKEAVKGVDFVHTDVWVSMGEPVDVWGERIEELLPYQVNMDIMKATGNPRAKFMHCLP
                     AFHNSETKIGKQIAEQYPQLAGGVEVTEEVFESPYNIAFEQAENRMHTIKAVLVSTLG
                     DI"
     gene            complement(2621..3871)
                     /gene="arcA"
                     /locus_tag="VFF22_02920"
     CDS             complement(2621..3871)
                     /gene="arcA"
                     /locus_tag="VFF22_02920"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HZX15805.1"
                     /translation="MSKKALGVHSEAGKLHKVLVCSPGLAHLRLTPNNCDELLFDDVI
                     WVSQAKRDHFDFMTKMRERGIEVVEMHNLLEETVRNPEALKWILDRKINPNSVGLGLQ
                     DEVRSFIEGLEPRRIAEFLIGGVSGADLAKHKDSDAAKMFNAYLGESSFILPPLPNTQ
                     FTRDTTCWIYGGVTLNPMHWPARRQETLLTTAIYKFHPDFVNEEFDIWYGDPDQDHGS
                     ATLEGGDVMPIGNGTVLIGMGERTSHQAIGQVARALFAKGAADKVVVAGLGRSRAAMH
                     LDTVFSFCDRDLVTIFPEVANSIVPFILRPDESRPGGIDVRREEKSFLDVVAESLNLK
                     KLRVVETGGDAYEAEREQWDDGNNVVCLEPGVVIGYDRNTYTNTLLRKAGVEVITISA
                     SELGRGRGGGHCMTCPIIRDPIDY"
     gene            complement(3888..5315)
                     /gene="arcD"
                     /locus_tag="VFF22_02925"
     CDS             complement(3888..5315)
                     /gene="arcD"
                     /locus_tag="VFF22_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HZX15806.1"
                     /translation="MPDSTQKLRLGALVALVVGSMVGGGIFSLPQNIAASASAGATLI
                     GWLITGVGMLTLAFVFQTLANRKPQLDGGVYAYAKAGFGDYMGFSSAWGYWISAWIGN
                     VSYMVLLFSTLGYFFPVFGEGNTLPAIICASVLLWLLHFLVLRGIKEAAFINTVTTIA
                     KIVPLALFIVIAAIAFKMDVFTSDFWGAGNSELGSVMDQVRNMMLVTVWVFIGIEGAS
                     IFSARAEKRSDVGKATVIGFIGVLLLLVLVNLLSQGIMTQAALAGLKNPSMAAVLEQV
                     VGPWGAMLISIGLIVSLAGALLSWTLLCAEIIFSSAKDHTMPEFLRKENVNHVPVNAL
                     WLSNGLIQLFLIITLFNDATYLKLLYLATSMILVPYFWSSAYAVLLAVRGETYENARG
                     ERNKDLLIALISTLYAVWLVYAAGVQYLLLSALLYAPGALLFAKAKHELGQPIFTGVE
                     KIICTAVLIGAALAAYGLYEGTLSL"
     gene            5553..9599
                     /gene="hrpA"
                     /locus_tag="VFF22_02930"
     CDS             5553..9599
                     /gene="hrpA"
                     /locus_tag="VFF22_02930"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HZX15807.1"
                     /translation="MTEATPAVDQLLKNLDQTLLGDRHRLRRQLHELRKQSPVDEAKL
                     AQWLERFEASRAKVEARRLSVPPMRYDDALPIAAKRDEIKAAISKHQVVVIAGETGSG
                     KTTQLPKICLELGRGVHGLIGHTQPRRLAARSVATRVAEEIGTPLGELVGYQVRFEDQ
                     SNERSLIKLMTDGILLAETQHDRYLEKYDTIIVDEAHERSLNIDFLLGFLKTLLVRRP
                     DLKLIITSATIDLQRFSEHFGGAPIVEVSGRTFPVDTWYRPLTAEQDEDGNRVEEDLS
                     VDQALLATLDEISAFEKAEGKRPGDVLVFLPGEREIRDAAEVLRKANLRLTEVLPLYA
                     RLTPAEQQKIFAPMGARKIVLATNVAETSLTVPGIRYVIDSGTARISRYSYRAKVQRL
                     PIEAVSQASANQRKGRCGRVEPGICIRLYSEEDFNGRPAFTDPEILRTNLAAVILQML
                     HLRLGEIEAFPFIEPPDGKAISDGFNLLQELSAVNREGQLTPLGRQLARLPVDPRLGR
                     MVLEGAKQGSLEEVLIIASALSVQDVRERPSDRQQAADQAHAQWKDVDSDFAALINLW
                     RGFEEQRQALGSSALRSWCRKNFLNYLRLREWRDAHRQLVLITRDLQLSEGGRGQGRS
                     GLGRDQQSAGQAAIAGDRQLSAAQKEKELAEKDQAAQRAKGYAAVHKAILAGLLSQIG
                     QKTEDGDYLGARQRRFWVHPSSVIGRKKPSWLMAAELVETTKLFARMVAKIEPDWIEP
                     LAGHLIKKNHLEPHWEKKRGQVVAFEQISLYGLIVVGRRPVHFGPIEPVVCRELFIRE
                     GLVGGEINSRAKCLTANRQLLEKLDELEAKARRRDILADDETLFAYYEARLPADIYQS
                     ATFDSWYKTESAKNPQLLIMREEDVLARDAKEVTAAQYPDSLRLDELTLPLSYHFEPN
                     HPRDGVTLRVPAPLLPQLPPERLEWLVPGLLEAKCMALVRGLPKALRKNFVPVPDFVG
                     AALGKISFADGSLPEALGRELTRMTGTRVSEEAWTESASQIERHLRMNIEVVDNQGKP
                     LGEGRDLAELTARFSEATQAGLALPKTAKSQRPVEAKAFSEVAAKTQQQVAGLSMTVY
                     PALVEEAGVVKEGRFPTLAEAEFQHRRALQRLLLQQLAEQAKFLRSKLPGLTELALLY
                     RELGRVDALVEDILLASLDSCILEGSEPLPRDGAGLAALAERKRGDWTGEAERLAKLT
                     LEILKLWHGLQKRFKGKIDLAQAMALNDIKLQLSNLVYPGFVRETPTQWLKEVPRYLK
                     AIEQRLDKIAGQVQRDRVWSGELAGYWEQYQARLGKHSQEGKRDPQLVLYRWLLEEYR
                     VSLFAQQLGTKLPVSDKRLSKQWSSVEG"
     gene            complement(9742..10947)
                     /locus_tag="VFF22_02935"
     CDS             complement(9742..10947)
                     /locus_tag="VFF22_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZX15808.1"
                     /translation="MKQQILIIGAGFAGMWSALSAVRLLDQQQRLDVNVTLLAPQAEL
                     RVRPRFYETDLASTVAPLGPLFEAVGVRFVQGSATQINAASQRVSYRDASGAEAELNY
                     DRLILASGSQVARPPLEGIERFTFDVDSLESAQRLEAHLQSLAQQPESAARNTVVVVG
                     GGFTGIETATEMPARLRAILGEQTDIRVIVVDMAKQVGGAMGEAIAGIVAEASDSLGV
                     TWRLGSPVSKVDEHGVQLGNGERIAASTVIFAVGVKASPLTQQINGERDAHGRLHVDR
                     NLKVLGQDAIYASGDTAYAAVDDQGNHALMTCQHAIALGRSAGNNAAADLLGVAPIAY
                     SQHKYVTCLDLGAWGAVFTEGWERKVLLTGQEGKVLKTQINTQWIYPPAADRAIALAA
                     ADPLIPVVA"
     gene            11072..12571
                     /locus_tag="VFF22_02940"
     CDS             11072..12571
                     /locus_tag="VFF22_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZX15809.1"
                     /translation="MPPEKSPPQVVFLPLGTPEPGVSLQRWLYEALRGAILGGRLPAG
                     SKLPSTRALAEHYKLARGTAQAAYQQLLSEGYLEAQTGSGTRVCQVLPDATLNAGYVR
                     RKVVATVDVPRKPPTTPWIERLEAINPVFARRPVMPGLRPFFPHRGDTQAFPVDLWRK
                     LHIQQLRASRLSILLDSDPGGLPALREAIAGYLAIARGVQVPAERILVLNSVQQGLDL
                     CLRLLAPGNSQVWMEDPGYPGARQLIDVSGVRRVDVPVDAFGIQVEEGVRLAPDARLA
                     YVTPSRQAPLGGELSPARRLALLQWAAQHGSYIFEDDYDSEYRFIAKPIPALRSMPAA
                     EQSVILAGTFSKLLFPSIRLAYLVLPAHLLESFTRAAALMSRHANSLSQAVLADFIHE
                     GHFDRHVRRMRKIYASRAEAFAESAQRHWRGLIDVPEIRAGLDITCNLQVDNEQAAFA
                     RLQAAGIDTMPLARYCVQPRKPGLVLGFAPYDERAIEDAAKAVALALKQ"
     gene            complement(12652..12978)
                     /locus_tag="VFF22_02945"
     CDS             complement(12652..12978)
                     /locus_tag="VFF22_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014595510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15810.1"
                     /translation="MAIHYTASAENVACGRTGSNLSSTQDVTQVSCKLCLRSVEKGTA
                     EPVRKTPSLAELRAAAKAAKAAAAAAAMVKPSTTLKPRAEWQQRLEQLPGKNRIPRGM
                     ARQVFV"
     gene            13204..13704
                     /locus_tag="VFF22_02950"
     CDS             13204..13704
                     /locus_tag="VFF22_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005894948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZX15811.1"
                     /translation="MARKTIDDLFVHELSDMYSAEKQISKALPRMARAASNPLLAEAF
                     TAHLEETQGQIERIDQLVELAGLKLKRMKCVAMEGLVEESKELIEEIDKGPVLDAGLI
                     GAAQKVEHYEIASYGTLIAMAKHIGIKAAAIQLLKDTLAEEKATDEKLTKIAEQGGNQ
                     AATMNS"
     gene            13813..14358
                     /locus_tag="VFF22_02955"
     CDS             13813..14358
                     /locus_tag="VFF22_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15812.1"
                     /translation="MNVSRIISPESAIVEASEASDPLMFGTPRERLDFYRREIQYETS
                     LLANRTNAYLSAQSFLVIAYASSMANANTQWGDLFTLVVPALLALLGIVSSLHAWPGI
                     KASADIIGHWHFKQDHLLRSEPKFGHAYDDSPLFCEQESNKDSYYKSLLFSLRSPWLF
                     AVFWGVLGAFSLWLQLSAVKL"
     gene            complement(14387..15079)
                     /locus_tag="VFF22_02960"
     CDS             complement(14387..15079)
                     /locus_tag="VFF22_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HZX15813.1"
                     /translation="MTAFDWQRMLMDDFPVLFLGEVALRALFAFVVVFLFLKVSGRRG
                     IRQLSLFELVMILTLGSAAGDVSFYDDVPLLPVLVVFATLLLLYRITVLSMSRSPRLS
                     KWMEGEPVTIIKDGLYELNSLDRLHISSDEFFMELRQQSVDHLGQVRLGILETDGDIS
                     LYFYATEETRPGLSVLPPEHRAVYERVPVSDLYACIRCGNTQWMEGQQHSLCARCEHG
                     SWSPALRHSRLH"
     gene            15365..15604
                     /locus_tag="VFF22_02965"
     CDS             15365..15604
                     /locus_tag="VFF22_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012075875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HZX15814.1"
                     /translation="MRMLKTLTFAVLHFSIAFTVAYMLTGDLLTGGLIALIEPACNTV
                     AYYFHEKAWLRFGSSSSAEHDGCGHGGLLRFFKRD"
     gene            complement(15960..17708)
                     /locus_tag="VFF22_02970"
     CDS             complement(15960..17708)
                     /locus_tag="VFF22_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003379986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retron Ec67 family RNA-directed DNA
                     polymerase/endonuclease"
                     /protein_id="HZX15815.1"
                     /translation="MPRLRALQKATTLHDLAVILNYSPTALAYLLYKKTGKYSRFQIP
                     KKNGTPREILAPCDELKSLQRKLKKLLDECLESIEKKRSPLGSLSHGFKANHSIITNA
                     EPHRKRLYVFNVDIKGFFDSIHMGRIRGFFISNKDFNLNTKTATILAQIICHDDKLPQ
                     GSPASPVASNLIGHLIDIRMVQLAKRLGCTYSRYADDITFSTNKSEFPPEIAYRLENS
                     HDWIPGGALLKVIYKSGFGLNHEKTRMQYARAQQTVTGLVVNQSTNTPAEFRRKVRAM
                     THKLFMDGEYYVHEENGPRSPKKTELNHSKLNKLEGMYSYIYMTDCFNRKKIIENSNR
                     RHEDFPLSSLEKLHGDFLFYKNFYASPAPTIICEGKTDNIYLTCAIKSLHEKFPRLAK
                     PDASGKVKLKVRFINYSELTHRVMGLNGGSSDLGHLIRRYAKQCGSYKAHPPLNPTII
                     VVDNDSGSQPIFQAIKDVTGDRYSIPSGKGKIFDKSQTLYKVAQNLYVVLTPLLNGKD
                     SMMEDFFPPAVLRATYNGKSFEAVSPGPKGTTYSKHQFSQHVVKPNQKIINFSRFSHI
                     LKSINLAIRDYPKIKL"
     gene            18365..19060
                     /locus_tag="VFF22_02975"
     CDS             18365..19060
                     /locus_tag="VFF22_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003201343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HZX15816.1"
                     /translation="MNEQTLSMRLERVAVHVPAGARLADIGSDHAYLPVALLRRGAIE
                     AAVAGEVALTPFHAAERSVRESDLGQRINVRLADGLAAIEAGDAITAISICGMGGERI
                     RDILDSGKARLSGQERLILQPNGGEQPLRQWLMENGYRILCEEVLRENRFVYEIIVAE
                     RSGPVTYSAEELYFGPLQMQTRSPAFLAKWQRMLRLKQQTLADFAHARQAVCEEKVQK
                     VAQQMQWITELLA"
     gene            19405..19662
                     /locus_tag="VFF22_02980"
     CDS             19405..19662
                     /locus_tag="VFF22_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HZX15817.1"
                     /translation="MTTFEVKDMTCGHCIKAITQAVLTVDPTAKVQIDLSSHRVQIES
                     ASAEAELSHAIVKAGYSPITKTAEQASFAPIKTTARVCCCH"
     gene            complement(19966..20442)
                     /locus_tag="VFF22_02985"
     CDS             complement(19966..20442)
                     /locus_tag="VFF22_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZX15818.1"
                     /translation="MKAVSPNDFVAINDVLCRFFLSFDRRDWASMTNCLSSLVFIDYS
                     SSGREKPSSMSGKDFVKRRENAVDNLAKHHSFSNLLLAYEQNEDSVSARCNYLILRFG
                     DSQSTDIGGENFFHSCGNYDFLLSRADDCWRISSITQHALQSWGNRNLHGGSRAGS"
     gene            21013..21504
                     /locus_tag="VFF22_02990"
     CDS             21013..21504
                     /locus_tag="VFF22_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15819.1"
                     /translation="MASPDKQQKRAQRAKAKAKQNRSGKSKANVVHPLLANPLINEPF
                     DDVEIDLSTFDFKDIEENGFDPAHFDDLFQAMKVGEGISLLAMCLVFLQYPVLELVVA
                     EEAEEAATDFMMGLLIVYRGVFHDEDEDAAVTWIGSDAFQHAYNEASMILQKKNARSS
                     ARA"
     gene            complement(21621..22394)
                     /locus_tag="VFF22_02995"
     CDS             complement(21621..22394)
                     /locus_tag="VFF22_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014591542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX15820.1"
                     /translation="MNLDLSGKRVLVTGGTKGVGRAVVELFLAEGAQVLTSARQASSD
                     LPAELFVQADLSTPEGCETLVAAAKQRLGDVDIIVHVLGGSSAPSGGFAVLDDAQWQR
                     ELDLNLLPAVRLDRALLPQMLERKSGVIIHVTSIQNRLPLPEATTAYAAAKAALSTYS
                     KSLSKEVSPQGVRVVRVSPGWIETEASVALAERLAQEAGTDYAGGQRIIMDALGGIPL
                     GRPAKPAEVADLIAFLASPRAASITGSEFVIDGGTVPTA"
     gene            complement(22391..22720)
                     /locus_tag="VFF22_03000"
     CDS             complement(22391..22720)
                     /locus_tag="VFF22_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019438801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZX15821.1"
                     /translation="MNLQLPDAVQTYFDVSNGGDISRLAACFYADAKVTDENRTHQGI
                     AAIKAWQQEARQAFTYSLEPLAATQKDDTLSVTARLVGNFPGSPLQLNHLFLLQDGQI
                     RSLEIAP"
     gene            22837..23754
                     /locus_tag="VFF22_03005"
     CDS             22837..23754
                     /locus_tag="VFF22_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014591540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX15822.1"
                     /translation="MADSRLGLNELEAVLAVARRASFRQAAIDLDVSTTALSNTIAKL
                     EASLETRLFNRTTRSVSLTEAGAMYLEQVGPALQDVRAALEAVRSHNVGPSGVLRINA
                     FASAARAALVPLVLEFLQRYPKVHIDLVTEGRLVDIVADGFDLGVRAENLVPSDMIVI
                     PLGQPQRYAVVGSPAYFAQHGRPRSPADLLQHQCMRIRLPNGAIYPWHFERAGEVTRL
                     DVTGQLTLDEPSVAKAVVQAGVGLGFFMQQDVQAELDAGQFERVLEEWTPPRDNLCLY
                     YPNRRNPSAALKAFTALARGNLPSKGLPG"
     gene            complement(23785..25224)
                     /locus_tag="VFF22_03010"
     CDS             complement(23785..25224)
                     /locus_tag="VFF22_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014591539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZX15823.1"
                     /translation="MDSALRFDISLSGALAAISQVGDLSMGQPLGHSQRVARLAQMLA
                     RATHGDGDHLTTAQQVALLRWSGCTANADGFAHLLGNDVDGRRAMLDQTLGAEQMNAV
                     HQAAPLAVVHCEVSGQIAKTLELGAAVEAGLCRIFETYDGTGRPLGVSHEYIPDVTYQ
                     VVLAGDLDILCRAHGLDAALSWISEQANRRYPAVLASMLVRNAKEWLAELEKTPDVQD
                     DTRLERPVSLTLVGDVIDLKLPWLAGYSRQVAQIAVEAARLWGLPEPAQMEIGKAALI
                     HGLGRATVSNQLWNTAGPLPYGATERIHLVPYWTHKASRQISELIIPGEIAAHAYERL
                     DGSGYFRGTSADAQCAAYRLLASAVAWVALTSARPWRPAHSESEAARLLKQDASRGLF
                     DGDICEAVIAAARGERRLPQPKSALLTTRECSILLEISRGASNKDVARRLEISPSTVR
                     THMESIFRKLGCSTRAAATLKGLTLGLIA"
     gene            25315..26088
                     /locus_tag="VFF22_03015"
     CDS             25315..26088
                     /locus_tag="VFF22_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX15824.1"
                     /translation="MKTFKSQLVTTAFALASTFVVSVSQAAEATPSVVIVHGAFADGS
                     DWAKVVPYLQAEGVAVTVVQNPLTSLADDVAATQRVLNNQSGNVVLVGHSWGGTVISE
                     AGAHEKVSALVYIAAFAPDAGQFTGEQGKGAPTPPGISQIKADSNGFLYLTPEGMAKD
                     FAQDLPAAQTAVMSATQGPIKASAFDERTTVAAWKTKPSWYLLASEDRMIHPDVQRSS
                     AKRIGATLTELGTSHVPQQSQPAEVAKVILEAVRSVDAQ"
     gene            complement(26254..26841)
                     /locus_tag="VFF22_03020"
     CDS             complement(26254..26841)
                     /locus_tag="VFF22_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZX15825.1"
                     /translation="MRVSKAQAQANREHIVETASAVFRERGFDGVGVADLMAAAGFTH
                     GGFYKHFGSKADLMAEASACSLSRSLASAEALDVQGFIDLYVTRDHRDGRGSGCTMAA
                     LCGDAARQPDELKVAFAEGIERTLQLLGEKYPTAPDSAPGDARVKVIDMLAQAVGAIM
                     LSRACPDDSPLADEILKACHAQMTAALPVPSGNER"
     gene            26988..28094
                     /locus_tag="VFF22_03025"
     CDS             26988..28094
                     /locus_tag="VFF22_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008035518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HZX15826.1"
                     /translation="MTDQQLFTPYTLGALTLANRVVLAPLTRNRAGIGLVPSEFAAAY
                     YSQRASAGLLIAEASQISQQGQGYQNTPGIYTQAQIDGWRKVTDAVHAQGGKIFLQLW
                     HVGRVSHVDLQANGAAPVGPSALRAATKVFVNNSFADVSEPRALEIGELPGIVNDFRQ
                     AAANAIAAGFDGVEIHGANGYLLDQFIKDGANVRTDAYGGSIENRARLLLEVTAAVVD
                     EIGADRTGIRLSPVSPANGVSSSDPQAQFDYIVDQLNALDIVYLHVVEGATGGPRDVA
                     PFDFAAMRQRFKNTYIANNGYDLDLATSQLAEDKADLIAFGRPFIGNPDLVERLKAGA
                     PLSAFNPATLYGGGAVGYTDYPTMTESNTAANPA"
     gene            28159..28947
                     /locus_tag="VFF22_03030"
     CDS             28159..28947
                     /locus_tag="VFF22_03030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX15827.1"
                     /translation="MSTRPTVLITGASTGIGAVYAERFAQRGHDLVLVARDQARLHTL
                     ATRLHSEHGVAVDVLQADLTQLSDLTAIEARLRDDASIGILVNNAGAAQSGSFIEQST
                     DSVAHLVALNTTALVRLASAIAPRLAKAGNGAIINIGSVVGLAPEFGMSVYGATKAFV
                     LFLSQGLSLELSPQGVYVQAVLPAATRTEIWDRAGIDINTLSDIMEVGDLVDAALVGF
                     DRREPVTIPPLQEGERWDVLQTARQGLLSQIRQSAVAQRYLTQA"
     gene            28989..29858
                     /locus_tag="VFF22_03035"
     CDS             28989..29858
                     /locus_tag="VFF22_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX15828.1"
                     /translation="MTLMNAQHLFGSRASFVAAANQSIRVDGTAFAYRDLGPRTEVPL
                     VVFNHWGAVLDNFDPRIIDGLAQTRRVIAINYRGIGGSGGAAPLTVAEMADDAIGLIA
                     ALGFAKVDLLGFSLGGFVAQDIALKAPGLLRKMILTGTGPAGGAGIDRVGAMTWPLML
                     KGLLTLRDPKFYLFFTASATSRSAARAFLQRLKERKLDRDKPATPRAMLRQLKAIKAW
                     GRQAPQDLGSLRLPVLIANGDQDAMVPTALSRDMAHRIPGAQLIIYDDAGHGGIFQYH
                     ADFISDALAFLAE"
     gene            29904..30905
                     /locus_tag="VFF22_03040"
     CDS             29904..30905
                     /locus_tag="VFF22_03040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZX15829.1"
                     /translation="MHAFIIDRYGKKAVGRIGEVPAPDVRGDDVLIQIHAASVNPLDS
                     KIKSGEFKLILPYRLPLILGNDLAGTVVRVGARVRGFKPGDEVYARPDDDRIGTFAEF
                     IAVKASSVALKPKNLSMVEAAAVPLVALTAWQVLVETAKLKKGQKVFIQAGSGGVGSV
                     AIQLAKHLGAFVATTTSTANIDWAKALGADVVIDYKKQDFASVLRDYDVVLNSLGNDE
                     LKKSLQILKPGGHLISISGSPTPTFAEARGLAWPLKQVLRLLSFGIRQKAKQSNVEYT
                     FVFMRADGVQLREITSLIESGAIRPVVDKIFPFADTHKALAYVDSGRAKGKVVVQVI"
     gene            31061..31957
                     /locus_tag="VFF22_03045"
     CDS             31061..31957
                     /locus_tag="VFF22_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX15830.1"
                     /translation="MKVLLAVAEAQSFAGGAKLVGMSPPSVTRVIAGLEKRLGTLLLA
                     RSTRSLRLTEAGRRYVEDCKRILLELEEAEELATGSSIRARGNLTVTAPVMFGELYLI
                     PLITEYLSAHPQVTINALLVDRLVNMVEEGVDVAIRIGQLPETGLQAIKVGHIRPVIC
                     AAPAFLERFGRPQEPNEVMAAPIVMSSASSLLTDWQFSLGTSSLTLHPKPRLMVSSNQ
                     AAINAACLGWGLTRVLSYQVAERVAKGELEIILETFEAAAIPVHVLYQGSGRVPAKVR
                     TFVDFCASRFLYDPALQKAGSA"
     gene            31961..32863
                     /locus_tag="VFF22_03050"
     CDS             31961..32863
                     /locus_tag="VFF22_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX15831.1"
                     /translation="MSRYRQMQVFAAVAQAGSLAAAARQLELSPATIMRSIAALEARL
                     NNTLLLRGPRGVELSPAGEQFAVSCRHLLEQATIAERSAAGMHASPAGRLTLSLPLLM
                     DQQVLMPIALDYLAAFPDVQLVTRASEGVPKLLEEGIDIALVVGHLPNSSGFAMPVGR
                     VQPIVCASPVYLAKWGRPQIPDDLKTHRAVLTTADGSQNEWRFRDERTTRLMKPAPVL
                     TCTTQRGAIHAATLGLGLIRCMSFEAHHELQSGLLEPVLSDFASQDLPAQLIYRDGRR
                     AEARVRTFIDFATPRLRAHPAFLG"
     gene            33015..33644
                     /locus_tag="VFF22_03055"
     CDS             33015..33644
                     /locus_tag="VFF22_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HZX15832.1"
                     /translation="MSQPAIKLYRVPLSGHAHRVELMLSLLQLPTELVFVDMANGAHK
                     KPEFLAINAFGQVPVIDDNGTILSDSNAILVYLAKKYGDGKWLAEEPLAAARIQRWLS
                     VAAGQLAYGPASARLITVFGAPLNAEEVIARSHALLKVMDGELASTPFLAGDSATLAD
                     IANYSYIAHAPEGNVSLQAYPNVRAWLERVEALPGFVPMQRTAAGLQAA"
     gene            33696..35753
                     /locus_tag="VFF22_03060"
     CDS             33696..35753
                     /locus_tag="VFF22_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009682585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZX15833.1"
                     /translation="MDHLPKHRRSPWHAGEKTLQETYGVSERMEVIGQKVIRDYMPDQ
                     HREFYQQLPFMVIGAVDAQQRPWATLLEGPEGFVTSPDPQQLLVAVQPDAQDPAAAGL
                     QPGQAIGLLGIELHTRRRNRINGVIQQVSAAGLAVAVEHSYGNCPKYIQARGYSRTPE
                     PLKSRASREDFTALNARTTEMIRAADTFFIASYFDHDAHSRSVDVSHRGGRAGFVKVE
                     GNRLTIPDYAGNLFFNTLGNLQANPVAGLLFVDFATGDMLQLTGRTELILDSPMIKAF
                     ENAERLWTFDVEQVVLRPAAVSLRWEFHDYAPTSLATGTWAEADEKLRQNEQRRQWQQ
                     WRVTEVRQESQDIRSFYIQPLDKAAVAFTPGQHMPLRLQTLGAEAPLIRTYSVSSAPS
                     DGHIRISVKAQGPASYHLHKQVQVGDVLEVRPPMGSFTLIEDTERPVVLIAAGVGITP
                     LLSMFRELAAQNIQQRQRPVHLFQSARTLEDLPFQAELKALQQRDTEHLHIHRALSRP
                     GTEAVVGRDFEITGRLDFAQVKARLPLDDYDFYLCGPGGFLQAMYDGLRGLNIVDERI
                     HAEAFGPSTLQRLAVEALAAPAQLLAASAPVPVYFSSSAKEARWTPGSGSLLELAESR
                     GLSPDFSCRGGSCGTCKTRLISGNVHYPNPPAQLPEGDSVLICCAVPAELDGAIQPLV
                     LDL"
     gene            35768..36013
                     /locus_tag="VFF22_03065"
     CDS             35768..36013
                     /locus_tag="VFF22_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15834.1"
                     /translation="MNREIVDRYDVLDASSETPGHWEVNYLLPQLLSGPEYPGRDVNA
                     ALMRVLKRLFADLRAPRVVPPVAPSLTPYRSHRHVYR"
     gene            36000..36476
                     /locus_tag="VFF22_03070"
     CDS             36000..36476
                     /locus_tag="VFF22_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007939704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZX15835.1"
                     /translation="MSTAEIRQPIPPFTRESALLKVRLAEDGWNSRDAVKVALAYTPD
                     TQWRNRAEFVASRAEAQAFLERKWCKELEYRLIKELWAFTDNRIAVRYAYEWHDDSGN
                     WFRSYGNENWEFAENGLMAHRFACINDLPIQESERKFRWPLGRRPDDHPGLSQLGL"
     gene            complement(36582..38285)
                     /locus_tag="VFF22_03075"
     CDS             complement(36582..38285)
                     /locus_tag="VFF22_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15836.1"
                     /translation="MVMEKCFAAVCFISLDPNGSKFFGFSEFLAGLALMVLAWTIGDV
                     RYHFRVQTAPIPLQRVTFSVVAIVGVLTLLTDLWRAQGWLVPQGSFLTPPLWQAFLAG
                     IFLLTFLTWTWFAFIRRPTFGKRNAERYAQALYRHILKGDPTELAVVADELTYSARAL
                     VHYATDRRMLRNHRRLQDEDELHPDLPKVEAYANDILLLIADKRLCRVIVESSSGTAL
                     AIFEEMGETKKYDIQVEIFAKNIVREALLNTNSFLYHEAMGYESGLIGYHKPLSQAMF
                     GNHKMVDTIRTLLDPDTVGKLKWNAVQWEAYCRLVLITLRDYVDKPHWNHSTALYRAK
                     DYIENAVSGLYKLNAVANTWESDAAKCLQVVMGFITDAVKILDKKGKPNQVRLRVRVE
                     YGHPRESLYDDIASMIFEVIFHASSVISPQWECWSIQHNSIWGPITRELNGPGGLVIK
                     FKLRRLLYDEIIEMNRFPNFKGAKILGFCLNVMGLEVRKGDYDKENRPLHKAILEWTK
                     KHYAWLQINTPRVAEACLVDGITYDAENLQLVKTYTADGLRREASYVRLELEPANPTV
                     E"
     gene            complement(38577..40286)
                     /locus_tag="VFF22_03080"
     CDS             complement(38577..40286)
                     /locus_tag="VFF22_03080"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZX15837.1"
                     /translation="MIEVTELPIAALRAALEAGQTTAVELVQAYLARIDAYDAAGTPT
                     QLNAVVVRNPDALKEAQASDERRTRGAALSPLDGIPYTAKDSYLVKGLTAASGSPAFK
                     DLVAYRDAFTVERLRGAGAICLGKTNMPPMANGGMQRGVYGRAESPYNADYLTAPFAS
                     GSSNGAGTATAASFAAFGLAEETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTM
                     DVVVPYARTMADLLEILDIVVADDPDNRGDLWRLQPWVPIPKASEVRPAAYADLAAKA
                     DALAGKRFGVPRMFINKDDAAGTSESPGIGGPTGQRIHTRASVIDLWEAARKALEAAG
                     AEVIEVDFPLVSNCEGDRPGAPTVYNRGIVTPEFLHDELWELSGWAFDDFLRANGDPK
                     LNKLADVDGPQIFPHDPGTLPNREGDLAAGMDEYVNMAKRGLKNWDQIETLSDGLRGL
                     EHTRKLDLEDWMDNLGLDAVLFPTVADVGPADADVNPESADIAWSNGVWVANGNLAIR
                     HLGVPTVTVPMGVMADIGMPAGLTFAGRAYDDSALLRFAAAFESTGSKRRVPPRTPPL
                     SRG"
     gene            complement(40375..41079)
                     /locus_tag="VFF22_03085"
     CDS             complement(40375..41079)
                     /locus_tag="VFF22_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HZX15838.1"
                     /translation="MSALAAYPITHKWPAQHPERLQLYSLPTPNGVKASIMLEEIGLP
                     YEAHLVSFDTNDQLSEEFLSLNPNNKIPAILDPNGPGGKPLALFESGAILIYLAEKTG
                     QLLAQDPTTRYETLQWLMWQMGGIGPMFGQLGFFHKFAGKDYPDKRPRDRYVAESQRL
                     LGVLDQRLQGRSWIMGEDYSIADIAVFPWVRNLVGFYEAGELVEFERFANVQRVLQTF
                     VARPAVIKGLVTPARG"
     gene            41215..41652
                     /locus_tag="VFF22_03090"
     CDS             41215..41652
                     /locus_tag="VFF22_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HZX15839.1"
                     /translation="MSLHGEYDNQNIFAQIIRGEAPCYKLYEDEDVLAFLDVFPQSFG
                     HTLVIPKRAAARNILEIDSDSLNKVMAVVQKLTGVIIAELEPAGVQVAQFNGAPAGQT
                     VFHIHMHIVPRYTGETLSVHAGGKATPEELAALQARLIKRIAG"
     gene            41761..42186
                     /locus_tag="VFF22_03095"
     CDS             41761..42186
                     /locus_tag="VFF22_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HZX15840.1"
                     /translation="MRLICAVLPLLLLAGCSSWKPAPEDVKPVPADRLLGYQAPLVQG
                     GELQVNRDFGGMGGGCYVAVLVDRKVAARIGVGEQVRFQVPVGTRVLSIGIDEMDDTL
                     CGMGRLRRELAVKVEPGSQQNFRIVSDNRKGFDILPITQ"
     gene            42344..43630
                     /locus_tag="VFF22_03100"
     CDS             42344..43630
                     /locus_tag="VFF22_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZX15841.1"
                     /translation="MKGLPIAIVGAGTAGLATALFLTRQGFSVRLLERVAELQPVGAG
                     VLLQPSGLAVLQQLGLFAECSGLGAAVSRLYGTSANGRVILDTRYAHWQPGSFGMGIH
                     RSVLLTALLNAARQAGVQIETGVHISRFTQHGSHVQLYRQDEQGVEQPFGEFAALVLA
                     DGVRSVLRGQMQVKQWLQPYPWGALWSIVPTPASSPTAQDATDLRQWYRDCAQMFGIM
                     PTGRTHLNREQALSSLFWSLPVAQFGAWREAGLAAWKAQVLQLAGSAAEPFVELIERP
                     EQLSQAVYADVRMQQWHDGRVIAIGDCAHAMSPQLGQGANMALVDAAALSAAVITHST
                     QANCDWSAVFAAYASSRRDHLRYYRQASRLLTPLFQSHSRSLAWLRDGVLLLARHNPF
                     GRAHAVSTLVGGRSGWLLAGAERPLQIWNGQANDGG"
     gene            complement(43747..44502)
                     /locus_tag="VFF22_03105"
     CDS             complement(43747..44502)
                     /locus_tag="VFF22_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15842.1"
                     /translation="MRNHLEALVPIFIDIEGERFSLFGSGVLIEFRGEAFLLTAGHVI
                     DGFERGALMVPHYDNKIVEIDGSYSYIDPSDGRNSDNLDFGYFKLEKSFAEGMKKLFY
                     AIPESEFGIKGDYSDKELMTFGGFPIRKHNVAGSVATTKEYAYGAYHAAASDYATLRC
                     KPRLNIVAKYNRNKTVDPSIGKIQYAPLPHGISGGGIFIWPPVLKDLIPDDRKLIGIG
                     HTYLKNGGYFIGTRLEIILTAILKNNPHLAIQT"
     gene            complement(44649..45119)
                     /locus_tag="VFF22_03110"
     CDS             complement(44649..45119)
                     /locus_tag="VFF22_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15843.1"
                     /translation="MIQESSYWKNDLLKLACRLERRLIQERWGEKNFYTVEKEIFIGF
                     YSVRKLIESKRVPDHISGKKYNIHEVPYCEKPTSLATNTKEAQYDFRALKNTEISIYD
                     LCNQFIHSHHFVPFIPGGKNLIGFFFCSDRKRKSGLYLITLFDVVDIYRLVGKS"
     gene            complement(45327..46223)
                     /locus_tag="VFF22_03115"
     CDS             complement(45327..46223)
                     /locus_tag="VFF22_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019582079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX15844.1"
                     /translation="MSNPDLNLLITLDAVLAEGSVARAAKRLRLSPSAMSRALARLRE
                     TTGDPLLVRAGRGLVPTPRAIELRDRVSQLVRDAQEVLRPVELLDLKKLDRTFAIRTS
                     EGFAENFGPALIARIGEQAPGVRLFFMQKPDKDSKPLRDGVVDLETGVIELASAPELK
                     GQSLFRDNYVGVVRASHPLSCSTITPEKYAASMHISVSREGLEKGPIDDALLPLGLER
                     KIATIVGGFSIALGLARGSDMIASVPERHTASLREGMYSFPLPFRTAEFTVSLLWHPR
                     FSADPAHRWLRNLILEVCGRSE"
     gene            46368..46931
                     /locus_tag="VFF22_03120"
     CDS             46368..46931
                     /locus_tag="VFF22_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15845.1"
                     /translation="MNSLSRFVEGVQSIWGPLSSEVVAGCQQQLDELLKTPVTEPWLA
                     DLRVQALAQQEMYRDPVNGFVLLAHTEPEGLYRPPHDHGRGWVIYAVQHGEIEMGTYA
                     KVQAPDGQVKLVKRGSTRVSAGQTQVYLPGDIHDTHCVSGPALLFRFTERDLKKEDKE
                     ARKLTRYIEQDGDWVPASQCAPERLKS"
     gene            46928..48310
                     /locus_tag="VFF22_03125"
     CDS             46928..48310
                     /locus_tag="VFF22_03125"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX15846.1"
                     /translation="MRLGPATKQHAVDARSDRTKPRHLAAISSVLAAMVLVVLNTAIA
                     NMALPTIAQSLQVTPALSIWIITANQAALLMALLPCAALGESLGYRRVFRWGVVLFLF
                     ATLMCALAPTLSWLIAARLLQGLGGAAVMALAVALLRQVLPSQQLGAAIGWNALAVAL
                     SSAAGPALGAAILSVADWPWLFAVLLPFGFWVMLAARALPAVDGTTRKADLLSIALNA
                     GTFASLVIAAGVITEHPALAAALLAISAIQLTLLVKRERQKESPLIPLDLLRSNSFRI
                     SVIASVLCFAGQTAGLVALPFYLQHSLGQEVLMTGLYMIPWPLTVALVAPLAGRLSNR
                     WSPAWFCSVGGICLSTGLAGAALWPLQENLMMLVPLTMLCGLGFGLFNVANNRNMFLT
                     APMSRSGAAGGMQGTARLLGQTAGAVIMTLLFNLFPAHSAPQLALGAGALLNLLAALI
                     STLQSRETYN"
     gene            48544..49002
                     /locus_tag="VFF22_03130"
     CDS             48544..49002
                     /locus_tag="VFF22_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019649302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX15847.1"
                     /translation="MPRLVWLRDHMQHSDTLAQWLHQQFAYEFIEQPLADWQREFAEG
                     QGNGDWQCLIALENDQLLGGAALAASDLSERPELGPWLACVFIAPQARKRGLAEQLIE
                     GICAAAKANGTTRLYLHTQDRNDYYAKRGWQVQECFEAWGKEQWLMLRDL"
     gene            49133..49444
                     /locus_tag="VFF22_03135"
                     /pseudo
     CDS             49133..49444
                     /locus_tag="VFF22_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011267488.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
     gene            49606..50457
                     /locus_tag="VFF22_03140"
     CDS             49606..50457
                     /locus_tag="VFF22_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019580203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZX15848.1"
                     /translation="MSSIAISDLTVSPNVDSVSRPVLALSATLLAKDWENSRHQHRKA
                     QLIYSVRGILNCEIEDGVWIVPPQCALWIPGDLPHSAWGSGEVECYCLFVEPDTALDL
                     PKTCCTISVSPLLRELLFKATAVPELYALGGREERLLAVLLDELVAAPVEDLHLPMPR
                     DARLRRLAEMILAAPADKTSKTTWATRIGMSERSMSRLLLHEIGMSFGRWRRQLHVIL
                     ALQRLTKGESVQTVALELGYENASGFVTMFRKAVGKPPARYLSDRTSGAVTTPVPGIM
                     LRDQVAL"
     gene            50750..53062
                     /locus_tag="VFF22_03145"
     CDS             50750..53062
                     /locus_tag="VFF22_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019580202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HZX15849.1"
                     /translation="MSDLATVGKSHWPIDIRAEADSYPLLEWTYKDGIRGAPLSLLGS
                     AVAAAILIGAQGAFAAEPAPTHNKAVGTSQGEIVLPAMSVTGQMNTAITEGTGSYTTG
                     ETAAATRLPMTLKETPQAVTVITRQRMDDQQLNTVKSVLENTTGVTARQADSERTSFY
                     SRGLLINNVQYDGIPTVVGNTVNGSGISALDTAFYDRVEVVRGGSALLTGIGNPSAAI
                     NLVRKRPTPEFAASASLGAGSWDTHRVMGDVSTPLTEDGRIRARLVGTYQDGNSYLDG
                     YKPERKAFYGIIEADLTTDTTVSLGYDYQDITPKGATWGGLPLWFSDGTQAEYSRSKS
                     YAQDWSYSDNTLKTAFSEVEHRFDNGWKLRAVANQYRSESEAELGSLSGSPDRATGLG
                     TFPSGAFPVALAAEGRSRQNTFDVMASGPFELLGRQHDLVVGATNSRRKAGQDDVAPF
                     FAGFTPVNIYTLSPGFARPDFDALPHVPTRTRIKQSGVYSAARFSLTEPLKFIIGGRF
                     NNYEVDDNVNGTSLHYKKNNEFTPYAGLVYDIDTTYSAYVSYTGIFNPQTEYRDRAGN
                     VLTPSKGKTKEVGLKAAYLDGRLNAAVAVFETELDNAAQQIPGAFTPSGARAYTGTDG
                     TKSRGIEFDLQGELASGWNIYAGIAHFTAEDGDGVRLNSQLPRTTAQFFTTYRLPGAW
                     NKLTLGSGVKWQSRFYQAPGAGTSTLGGEQSAYALTSVMGRYALSKNIDLGLNVNNLF
                     DKKYALQKGDFDTVTYGAPRNLMATLDYRY"
     gene            53132..53578
                     /locus_tag="VFF22_03150"
     CDS             53132..53578
                     /locus_tag="VFF22_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019580201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15850.1"
                     /translation="MPTPNPAQVLAIHNANKVTLFWLTTTVGAVLFVTFQLFFYVNNF
                     VRAQDTAPAITFEPSVLWVFPAFYGFWIVMVLLALIEARKAQWAVLILGSLLVILNTL
                     GGISDGLRDGWYVAFSAVFFITLPGVFAIAASWRHLNSKGTHHVDE"
     gene            53565..53966
                     /locus_tag="VFF22_03155"
     CDS             53565..53966
                     /locus_tag="VFF22_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012723489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15851.1"
                     /translation="MSMNSADFPLVWTSLAQEPGHDHQKDFDEFAANLQRGEPFVLLS
                     DTLPSEDHEHSPEEKKRTALWMKKHKAQLRSLVLALIMVEPSSAKRLGFKAFAGLFAK
                     VWGYPLLFAASREQAIEMANELLRKDKDQFH"
     gene            54064..54227
                     /locus_tag="VFF22_03160"
                     /pseudo
     CDS             54064..54227
                     /locus_tag="VFF22_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017846754.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            54308..54763
                     /locus_tag="VFF22_03165"
     CDS             54308..54763
                     /locus_tag="VFF22_03165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX15852.1"
                     /translation="MDCQIRYATRDDVAAISRVVVSALRASNAQDYPPDVIERVEQGF
                     SPEAILHLLTQRQIYVATVDAHVVATASLERDMVRSVFVAPAYQGAGIGRQLMAVIQL
                     VAASEGISLLRVPSSITAEDFYASLGFKQLRDEFHGAERTIIMEKRLDQ"
     gene            complement(55188..55916)
                     /locus_tag="VFF22_03170"
     CDS             complement(55188..55916)
                     /locus_tag="VFF22_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX15853.1"
                     /translation="MHKLLPALLLLTLSCASFASERALRFSINDSWAMPMVRIEDGKA
                     VEGILVDLQLRMAAKVGRKAELVVMPRLRVQQALDTGEIDVRCYVSPNWINSGHYRYI
                     WSLPFMTQRDVLAGITPEKLEPEQLLNERLGTVLGFTYPRLEPLFASGQIQREDARTQ
                     DQVLLKLSARRYSYAISNELSLHWFNRHQPPGQKLHLLSEVASDPIACIVRDAPDVPT
                     MALLRAMVQMKQAGEFDGILARYR"
     gene            complement(55946..56557)
                     /locus_tag="VFF22_03175"
     CDS             complement(55946..56557)
                     /locus_tag="VFF22_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HZX15854.1"
                     /translation="MALHTWLIFLTAVFGLSLTPGPNGLLALSHGALYGHRKTLFTVS
                     GGVLGFTLLMALSMLGIGTLLQASAQALTILKWLGGAYLIWLGIQLWRAPPLHLAMAE
                     KATESNGFKLFRQGLLSALSNPKVILFFGAFLPQFLDPRGDLWLQFAIMALTFAVVEG
                     GVEYLLARMAHRVRPWLQRTGKGFNRCCGGMFALMGAALPMTR"
     gene            57207..58328
                     /locus_tag="VFF22_03180"
     CDS             57207..58328
                     /locus_tag="VFF22_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009876827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HZX15855.1"
                     /translation="MHNVVISGTGLYTPANSISNDELVESFNAFVQQFNADNAAAIAS
                     GEVEALSESSSGFIEKASGIKSRFVMDKAGILDPKRMAPRIPERSNDDWGILCEMAVG
                     AAKDAMARAGKTAADIDGVIVACSNLQRAYPAVAIEVQAALGIQGFGYDMNVACSSAT
                     FGIQAAANSVQLGQARAILMVNPEICTGHLNFRDRDSHFIFGDAATAVIIERADQATS
                     NYQFDIVGTKLITQFSNNIRNNFGFLNRCAEEGVGARDKLFVQEGRKVFKDVCPMVAE
                     LIAAHLAENKLNVGDVKRFWLHQANLNMNLLIARKLLGRDAEPGEAPVILDTYGNTSS
                     AGSVIALHKYQADLPSGALGVLSSFGAGYSIGSVILRKH"
     gene            58328..58939
                     /locus_tag="VFF22_03185"
     CDS             58328..58939
                     /locus_tag="VFF22_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZX15856.1"
                     /translation="MDYPADQALLTRLRAGEQKAYRELVATYQGAMRAVAFAIVGSRN
                     ADEVVQDAWLAVVRSLDGFQARSSLKTWLLTITANTAKTRLKGNRREVLLDDIPSPHG
                     SIGDERFASDGHWLLAPHAWHHDSPEALLTEDELRECLEKTLASLSELQGSVLHLRER
                     EGLELEEICNLLQVSLSNVRVLLHRARLKVFATLEHFEETGQC"
     gene            58933..59166
                     /locus_tag="VFF22_03190"
     CDS             58933..59166
                     /locus_tag="VFF22_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009618502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZX15857.1"
                     /translation="MLSCKELVAHSSEFLDGQLGLRKRMSVRLHLAMCQHCRRFIRQM
                     RLSQAVLRQLPPGKSAELDALAAKLAELRRSNS"
     gene            complement(59200..59733)
                     /locus_tag="VFF22_03195"
     CDS             complement(59200..59733)
                     /locus_tag="VFF22_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15858.1"
                     /translation="MKAQLEELISKISSGCMLPEEVARIATEAAQAYTDPQAFLAANP
                     DINFDDSFPIPLGEWVVVGSLPETVLFQADSYDALFQQITESFGPEVSFVLKPKQLLK
                     VEPLKALNRIQVQLSSLSPETKGYVLLDFSEPLDDELQAVLVYSTDLARVMELAVEVG
                     IYAAPAYEALKAAQEAE"
     gene            59983..60711
                     /locus_tag="VFF22_03200"
     CDS             59983..60711
                     /locus_tag="VFF22_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007977083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZX15859.1"
                     /translation="MSAAPHPQQNHLLAALPNEVLERLLPHLELVDMPLGMVLYESGD
                     TLRHVYFPTDSIVSLLYVMESGASAEISVVGNDGVIGVALFMGGESTTSRALVQSAGS
                     AYRVLGKRFKEEFNRHGDMLQLMLRYSQALITQMAQTAVCNRHHSIDQQLCRWLLLSL
                     DRLTGSKLAMTQELIANMLGVRREGVTEAAGKLHRLGVIDYSRGLITVLDRDKLERLS
                     CECYAVVKRETDRLLPYKVALSAH"
     gene            60864..61568
                     /locus_tag="VFF22_03205"
     CDS             60864..61568
                     /locus_tag="VFF22_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZX15860.1"
                     /translation="MSVKAPLHILSRLIEGLPVKQRKQLLDGCEPVDLVFGNVLHEAN
                     QPIRHVYFPLSGFISLVTTLDGHQPLEMGLIGNEGMLGATLALGIGQAPMRAVVQGSG
                     TALRISSQLFKQELLSSPALLRVLKRYLYVVMAQLSQSAACTRFHAIEPRLARWLLMT
                     HDRAHADHFHLTHEFLADMLGVRRSGVSIAAAAMQARGLISYSRGEIKILDRAGLEQA
                     ACECYAALQADYHAQF"
     gene            complement(61613..63730)
                     /locus_tag="VFF22_03210"
     CDS             complement(61613..63730)
                     /locus_tag="VFF22_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZX15861.1"
                     /translation="MANRVLIITGNTDDAATLEDVLNRAQDGPFLIEWVTSLARALER
                     LETHRKEPIDAIMVDLDLPDSQGMDTFDQLFALASSIPILTLSALEDDQQTWDAIQRG
                     AQGCLSKGHFSSYLVPQSLRNIIQRKAVEEALFVEKARALLTLNSISDAVISTDLQGN
                     VDYLNNAAEQMTGWLLEEAQGRPIDEVMRLVNDKTRETVPNPIFKVLQQDEAKLLAAG
                     TILIRRDGSEAAIEDSAAPIHDSAERIKGAVMVFHDMTAAQAMTEKMAHLAQHDALTN
                     LPNRALLNDRINQAIALAERSGSSLALLFLDLDNFKHINDSLGHGVGDQLLQAVALRL
                     SDCVRSADTVSRLGGDEFVVLLSENQTDEDATLTAEKILNTLAMPHNIGQQLHVTSSI
                     GISTYPNDAKDAESLIKNADTAMYQAKEEGRNNYQFFRHEMNIHAVERQLIEAHLRTA
                     LENNEFVLHYQPKIDLQSGRITSAEALIRWQHPVWGMVLPARFIPVAEACGLILPLGR
                     WVMLQACAQAKRWENCGYNLDSVAVNISSMEFRRKDFVGSVANALTASGLAPDRLQLE
                     ITESVLMRDAQASATILHQLKILGVQLAVDDFGTGYSSLSYLNQFPIDVLKIDRSFVQ
                     DIHNSNGIIVSAVIAMGNSLKQRVIAEGIEETAQLEFLRHHHCAEGQGFLFSHPVSAE
                     SFGDLLRIGLSSPIHTLYEATSA"
     gene            complement(63831..64130)
                     /locus_tag="VFF22_03215"
     CDS             complement(63831..64130)
                     /locus_tag="VFF22_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15862.1"
                     /translation="MHTLGHTHALPSSIALILLALLAGNASAELFAPEVFSRAPQPAT
                     TAEAARSSNLHNARLQPEKDHTELARSTVRLNSRWISLPVKAPQHDQSPPSGLRF"
     gene            64544..66196
                     /gene="pgm"
                     /locus_tag="VFF22_03220"
     CDS             64544..66196
                     /gene="pgm"
                     /locus_tag="VFF22_03220"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HZX15863.1"
                     /translation="MSPRIHPLAGQPAPASLLTDISALLAAYYDLQPDLSVASQRVAF
                     GTSGHRGSSLTHSFNQAHVLAISQAICDYRVAQGISGPLFIGADTHALSAPALDSAIE
                     VLAANGVQTMISTGGEFTPTPAISQAILVHNQGRSSGLADGIVITPSHNPPDSGGFKY
                     NPTNGGPADSDITTWVQNRANALLEGGLREVKRMPLAQARQAANVQEYDYLGSYVSDL
                     GNVIDFAPIRAAGLRLGVDPLGGAGVHYWARIAEQYGLNLDVVSEIIDPQFAFMSLDW
                     DGQIRMDPSSSHAMQRLIGLKNGYDIAFACDTDYDRHGIVAPSVGLLPANHFLSVAID
                     YLFQHRPQWSATAAVGKTLVSSAMIDRVAARLGRPLQEVPVGFKWFAGGLFDGSLGFG
                     GEESAGATFLRRDGRVWTTDKDGIALALLAAEMTAVTGRDPGELYQGLSDEFGAIYAD
                     RVDAPATPAQKKALGKLSPAQISSRELAGDAITQVLDKAPGNGAAIGGIKVISEGGWF
                     AARPSGTEDIYKIYAESFRDAAHLQRIFSEAQQIVDGALVAE"
     gene            66325..66483
                     /locus_tag="VFF22_03225"
     CDS             66325..66483
                     /locus_tag="VFF22_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HZX15864.1"
                     /translation="MSLGTILLIVLVLLLIGAIPAWPHSRSWGYGPTGGLGVVVVVLL
                     VLLLMGRI"
     gene            66591..66956
                     /locus_tag="VFF22_03230"
     CDS             66591..66956
                     /locus_tag="VFF22_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZX15865.1"
                     /translation="MYRLDLKIRLHNGADIAIGPGKAELLSAIAQHGSISAAARAMGM
                     SYRRAWLLVETMNRSFKQPLVSTLVGGKQGGGTQLTATGELVLQRYRAICAAAMAAVQ
                     SGCDELAELLAEPPANNVL"
     gene            complement(66951..75455)
                     /locus_tag="VFF22_03235"
     CDS             complement(66951..75455)
                     /locus_tag="VFF22_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014819967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HZX15866.1"
                     /translation="MTSVFSALFSRAAAPSPWFDHAPVREELFGIERLEQHAYTLAAA
                     QPVSSKPPAVLSLHRRLNDNAKVLLAAYRASAAELANGRGVVPAAEWLLDNYHLVEEQ
                     IREIRDDLPPGYYRQLPKLASGPFAGYPRVLGMAWAFIAHTDSHFDPDILQRFIVAYQ
                     QVQPLTIGELWAVAITLRIVLIENLRRLADQMTVGRSARADADALASRLLGPDAPHAA
                     LDKELHERARTALSEPFAAQLAKRLRDHDPLTTPALGWLEARLGAQGSSTDEVVQHAQ
                     QRQGASNLTVRNIINSMRLISSIDWAQLFENVSLVDARLGARSDFAAMDFATRNQYRS
                     AIEQLARGSTCSELEVAEQALQATQQARDTQHADPRTTDPGYHLIAEGRLALEQRINF
                     RPPLRLRISRGHIRLGMPGYVSAIVGLASLLLGLALYSLAGQGVAAGWLLALGVLGFL
                     PATEVATALVNRAVSNSFGAMGLPGLELSEGVPSNLRTLIAVPTLLGSEADLLEQIER
                     LEVHHLAGHGGDLTFALLTDGLDAEQEHLASDDALLQLASTAIDQLNRRYGPGIAGSR
                     FLLLHRRRQFNPSENVWMGWERKRGKLHELNRLLRGATDTSFTEVPGLSQRVPADVRY
                     VITLDADTRLPRDAALRLIGKMAHPLNQPRFDTALQRVVGGYAILQPRVTPSLPLQRE
                     GSLYQKVFSSPGGMDPYAAAVSDVYQDLFGEGSFTGKGIYDIDAFEAALAGRVTENSM
                     LSHDLFEGVFARAGLASDVEVVEEFPSRYDVAGKRQHRWTRGDWQLLPWILGPFSGKQ
                     ALPSIGRWKMLDNLRRSLLAPATLAALGLSWLLPLPAALSASLLLIASLLIPALLPLC
                     FGLLPARAGIRLRSHLDKLRDDLLLALLQCALNLTFLADQSWRMLDAVSRTLTRLLIT
                     RRNLLEWTTAAQASISPRLKLLGFYCGMAGGTVLGLLLCALAVRWSPAVWPLVLPFAL
                     LWLSAPAIALRVSRSPRVAKRLLVDPEQTSELRLIARRTWRFFETFVTQADNQLPPDN
                     FQEDPKPVIAHRTSPTNMGLYLLSAIAARDFGWAGTQRTLTRLQATLAVMEQLQRHRG
                     HFFNWYDTQDLRPLAPAYVSSVDSGNLAGHLLVVANACEEWLKAPVSADARQGIHDNL
                     QLACAALAALPAASSERSQQLHDLLQQIEAALNGTQPLHSLLPTLLRLSDKATQLSSG
                     VAPLDTNNQAGELLFWLQALTQALGDLQQDAELTDSQRTTLHDELRSVAEQARGMALA
                     MDFAFLLEPERKLLSIGFCLADNSLDSSCYDLLASEARLASLFAIAKGDVSTRHWFRL
                     GRTATPLGSGSALISWSGSMFEYLMPSLVMRAPAGSLLEQTNRLVVARQAAYGEALGI
                     PWGISESAYNARDMEQTYQYSNFGVPGLGLKRGLSANRVIAPYATGLATMVDPQGALA
                     NYRRLAAKGALGRYGFYEALDFTRTRLPDDQPFAIVRNFMAHHQGMSIVAIANTLHDG
                     QMRARFHREPMIQACELLLQERMPRNVAVAHPRAEEVSACAVETTNEQHNQRRLNHVE
                     GNPPVTHLLSNGRYSVMLTASGAGYSRWRDIAITRWQEDSTRDQHGSFIFLWDTRNGT
                     HWSLGAQPSSGNPYDEVVFAEDYASYSRHDGNLTTSLEVLVSGEDDGEVRRVSLTNSG
                     RVAREIELTSYAELVLASAASDNAHPAFSKLFVVTEYLPAFAALIATRRPRSASEPSL
                     WAAHFAVVEGEVSAEPQYESDRAKFLGRSCALQAAAVIRQGQRLSNSVGTVLDPIFSL
                     RQRLLIEPGKIARVAFWTVVASSREALLDLIDKHHDRSAFERAKTLAWTQAQVELRHL
                     DMLAAEAADFQRLAAPILYADSRFRAPPASLLRGLGSQSVLWSQGISGDLPIVLLRIS
                     DAEDIAQVRQLLRAHEYWRMKRLAVDLVIVNEHASSYLQDLQVAIETAVRSSQSRPRF
                     AAELAQGSVFVLRADLLSGEYRERLQAVARVLLVAHRGPISEQLARIPPCPSPVPPSA
                     SNHSAQPIPVFHAPPPSPLEFANGLGGFDLDGREYVVRLDAWSNTPAPWINVIANPQF
                     GFQASAEGSGYTWADNSRENQLTPWSNDPVSDPPGEALYVRDEDSLALFSPTAQPLRD
                     SGYYEARHGFGYSRFSHQAGGIAMELLQYVPLEDPIKISRLTLRNLSGRPRRLSVTAY
                     AEWVLGSTRAASAPFIVSQQDSTTGAILVRNPWSIAFTGRNAFSDLGGQQNSWTADRS
                     EFIGRNGNLTQPAALARLAPLSGSLGAGLDPCTALQCQLELQPGESVEVLWLLGQCAS
                     EQKASALITQYREADLDAVLLKVQAYWQRVLGAVQVQTPSRAMDIMLNGWLLYQTLAC
                     RVWARSAFYQASGAYGFRDQLQDGMALTFASPSITRGHILRAASRQFVEGDVQHWWLP
                     HSGQGVRTRISDDRVWLAYATAQYIQCSGDAAILNEQLDFLEGPLLATGEHDAFFQPM
                     QADAPASLFEHCARGLDQCLQLTGELGLPLIGGGDWNDGMNRVGEHGKGESVWLGWLL
                     LRTIALFAPYAEVYDSARAARWRAHAEQLRLALETYAWDGKWYRRATFDDGTWLGSVQ
                     SSECRIDSIAQSWAVLSASADPLRVNMAMASMQRQLIREDDGIALLFTPPFDKTHLDP
                     GYIKGYPPGLRENGGQYSHAAMWAILAYCQLGSAQQAFALFDLVNPINHGNSPEACQR
                     YKVEPYVLAADVYAVAPHNGRGGWTWYTGAAGWMYRAGVEGILGIRREGDWLLLNPCI
                     PADWPGFSARIALAGSHYQIELSNPAQRSQGIRQAWLDDQPIDCSQGSLKVALDGAAH
                     RLRIEL"
     gene            complement(75760..76647)
                     /gene="gluQRS"
                     /locus_tag="VFF22_03240"
     CDS             complement(75760..76647)
                     /gene="gluQRS"
                     /locus_tag="VFF22_03240"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HZX15867.1"
                     /translation="MNTPAYIGRFAPTPSGYLHFGSLVAALASYLDARAVNGRWLLRM
                     EDLDPPREVAGAQAGILSTLENYGFEWDGELARQSERHAEYAALVERLLSQGLAYACT
                     CSRKQLEGTQGIYPGTCRNAGHGFADAAIRLRVPELTYHFVDRVQGDYRQHLGREVGD
                     FVIRRRDGLYAYQLAVVLDDAWQGVTDVVRGADLLDSTPRQLYLQELLGLPQPRYLHV
                     PLIIQPDGHKLGKSYRSPPLPADQATPLLIRALRALGQPLPDDANYGQPGELLHWASQ
                     HWDVSLIPRSLTLAEAQLR"
     gene            complement(76711..77154)
                     /gene="dksA"
                     /locus_tag="VFF22_03245"
     CDS             complement(76711..77154)
                     /gene="dksA"
                     /locus_tag="VFF22_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HZX15868.1"
                     /translation="MPTKAKAKSSQLTRGFEPYKETKGEEYMGEPMRAHFTGILNKWK
                     LELMQEVDRTVHHMQDEAANFPDPADRASQEEEFSLELRARDRERKLIKKIDETLQLI
                     EDNDYGWCDSCGVEIGIRRLEARPTATLCIDCKTLAEIKEKQIGS"
     gene            77350..78531
                     /locus_tag="VFF22_03250"
     CDS             77350..78531
                     /locus_tag="VFF22_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZX15869.1"
                     /translation="MAQSYSARSRAIEPFYVMALLARANQLQADGHDVIHLEIGEPDF
                     TTAAPIVAAGQAALANGHTRYTAARGLPALREAIAGFYAQRHRLSIDPERILITPGGS
                     GALLLATSLLVDPGKHWLLADPGYPCNRHFLRLVEGAAQLVPVGPDVRYQLTPDLIER
                     YWDQDSVGALVASPANPTGTLLSRDELGGLSQALKERGGHLVVDEIYHGLTYGVDAAS
                     VLEVDDDAFVLNSFSKYFGMTGWRLGWLVAPPAAVPELEKLAQNLYISAPSMAQHAAL
                     ACFQPQTLDILEQRRHEFQRRRDFLLPALRELGFKIAVEPEGAFYLYADISAFGGDAF
                     AFCQHFLETEHVAFTPGLDFGRYQAAQHVRFAYTQDLPRLQQAVERIKRGLISWQSHA
                     V"
     gene            78521..79228
                     /gene="sfsA"
                     /locus_tag="VFF22_03255"
     CDS             78521..79228
                     /gene="sfsA"
                     /locus_tag="VFF22_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HZX15870.1"
                     /translation="MQFDPPLEEGRLLRRYKRFLADIETTSGELLCIHCPNTGSMLNC
                     MAENSKVWFSRSNDPKRKLPGTWEIGETPHGRLAVINTGRANALVEEALRAGLIAELN
                     GFTSLKREVAYGQERSRIDFRLDYPTGAAFVEVKSVTLGFEGIRVAAFPDARTERGSK
                     HLRELAVLAREGIRAVQLYCVNLTDIDAVRPAEEIDPAYAAALREAVSAGVEVLAYGA
                     AISAQGVQLIRRLDVLL"
     gene            complement(79251..80429)
                     /locus_tag="VFF22_03260"
     CDS             complement(79251..80429)
                     /locus_tag="VFF22_03260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZX15871.1"
                     /translation="MSTASLHIALISETFPPEINGVANTLGRLVDGLRGRGHRVQLVR
                     PRQSVDPRGAADQDLSLTRGWPLPGYPGLQWGQSSMHKLLRNWQRQRPDVLYIATEGP
                     LGLSALRAARRLQIPVVSGFHTNFQQYTGHYGVGLLTRALTNYLRWFHNRSQMTLVPS
                     VSQQIELQRRGFDNLELLSRGVDSQLFHPTKRCTALRERWGLAEQDIAILHVGRLAAE
                     KNLALLSKSFQALQQAHPQRRLKLILVGDGPQRASLQQQLPDAMFCGLQRGEELAAHY
                     ASGDLFVFHSLSETFGNVVLEALASGLGVVAFDQAAAAQHIRHGHNGALAVAEDEQGF
                     IDAASWLLTDHEGLRRVRLNARLHASRQGWPAIVELFEQHLRSAMQPAQALPAGRTQH
                     "
     gene            complement(80488..81390)
                     /locus_tag="VFF22_03265"
     CDS             complement(80488..81390)
                     /locus_tag="VFF22_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX15872.1"
                     /translation="MITNLCQLDLNLLLVFDALMQEGNLTRAAERLHLSQSTVSNALA
                     RLRQQLGEELFQRTARGMTPTARAQALYVPVRQALRLLQTGLGPAEPFDPQTPHAFSL
                     SLNDYAQAALLPMLQAHLARNAAQVELLVQADDADSLVQRLESGALDLAIDYLHFDSP
                     DLHYTPLREEPLVAIGRAGHPAFSDGLSLEDYQQARHITVTPRAGRGSPLEIVLGSAR
                     VRRQAALHLPNYLPIPAIVAQTDLLGTVPARLAQAFAPVHALEIAPLPFDMPPVQVSL
                     IWHHQQHLDPAHAWLRQRLIELLD"
     gene            81473..>81884
                     /locus_tag="VFF22_03270"
     CDS             81473..>81884
                     /locus_tag="VFF22_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZX15873.1"
                     /translation="MAYNNDNAQTFRTRYRDAIHPLYNPWLHAVFVLAYGLTCISLLW
                     RTLDAVALWQWLLVPLSLVFFSWGEYQVHKRLGHNKTRFGQLFYKRHTGDHHSFFVEG
                     QMRYETPRDWRVILFPAWLIVLYSLPLLGIWWLLA"
BASE COUNT        15671 a        24065 c        25517 g        16631 t
ORIGIN      
        1 catcgcggcc aaggccgctc ctacaggtat ttcagcgctt cagcgcagtg cagccaaaaa
       61 ccataaccct cgccataatg cgccccgcgc ctatgcgcca agcggatcgc cgtgaatggc
      121 ctgggcgatt gttacaccgc ccgcttagtc gatatggtcg agattgacgg gctcccccgg
      181 cttggcatgc agctgctggc gaatatcggc gaacactttg tcgtattcgt cgtggccgcg
      241 catgatcggg ctggcattgg gtggcaggcc gtgcttagcg gcgacccggg tggcggcgct
      301 ggcgaagttg aaggcaacat cgcgatgcag ctcgaactcc tcgtcgaaat gccgtccctg
      361 cacctcaccg accaagcgca tatgcagcat tgggccctct tgcgggtctt ggcgcacttc
      421 gtagtagaaa tcgatggaaa agggcggagc cagccgcagg ccttcagaga ggtctaggtt
      481 gctgcggtgg atatggccag gtgtgaacat gctgagcgct ccattagggc gcgcagggcc
      541 gaagccctgc ttcgggtcag tagaatcgga tgccgtcgct tgccaggctg atgcgggtgc
      601 cggaggtgcc ctggacgatg gcttcgatat tttctagcga gccgatcacc gcggtgccgc
      661 cggtgttacg gacgaactcg caagcggctt gtaccttggg tcccattgag ccagccgcga
      721 agccgagctt gtccatctcg tcggggtgcg cttcggcgat ggatgtctgc gttggcttgc
      781 cccagtcgat ataggcggcg tcaacgtcgg tggcgatcac cagtaggtcg ctattgagct
      841 gttcggcgag cagtgccgaa cacaggtcct tgtcgattac cgcctcgacg ccgcgcagct
      901 ggttaccgtc gtacatggtg ggaattccgc cgccgccggc gcagatcacc acactgcctt
      961 tttccagcag ccatttgatc gggcggattt cgaagatgcg ttgtggcctt gggctggcca
     1021 ctacgcggcg aaatttgtcg ccgtcggggg cgatactcca gcccttttcg gcggccaggc
     1081 gctcggcctc ttccttgctg tagaccgggc cgatgggttt gctcggtttc ttgaaggccg
     1141 ggtcgccgct atccacctcg acctgggtga ggatggtagc gaagggcact tcgaacggca
     1201 gcaagttgcc tagttcctgc tcgatcatgt agccgatcat gccttcggtc tcggcaccga
     1261 gcacgtccag cggaaaggga ttgctggggt cgtaggcgtt gccttgcagt gccagcaggc
     1321 cgacctgtgg gccattgccg tgggcgatca ccagttcgtt gccgggtgcc accttggcga
     1381 tctgctcgca agcgatgcgc acgttctcgc gctggttctc ggcggtcatg gcttcgccac
     1441 gccgtagcag ggcgttgccg cccagagcga tgactaatcg catggttgga tctccttgtt
     1501 cgatagtgcg tagcccgaat gtatccgggc tacgtttctc cggcggctca gaggcgtcag
     1561 atatcgccca gagtcgagac caatacggcc ttgatggtgt gcatacggtt ttccgcctgc
     1621 tcgaaggcga tgttgtaggg cgactcgaag acttcctcgg tgacttcgac accgcccgcc
     1681 agctgcgggt actgctcggc gatctgtttg ccgatcttgg tttcgctgtt atggaaggct
     1741 ggcaggcagt gcatgaactt ggcccgcggg ttaccggtgg ccttcatgat gtccatgttg
     1801 acctggtacg gcagcagctc ttcgatgcgc tcgccccaaa cgtctactgg ctcgcccatg
     1861 gacacccaga cgtcggtgtg aacgaaatcg acgcccttga ctgcttcttt cgggtcttcg
     1921 gtgaggctga tgcgtgcgcc gctttcctcg gcaaagtgct gacacgcggc gatatgttcg
     1981 gcgctcggcc acagcgcctt tggggcggcg atacgcacat ccatgccgag cttggcaccg
     2041 atcagcagca gcgagttacc catgttgttg cgtgcatcgc ccaggtaggc gtagctgatt
     2101 tcgtgcaggg ccttgtcgct gtgctcgcgc atggtcatca cgtcggcgag catctgggtc
     2161 gggtggtatt cgtcggtcag gccgttgaac accggcactc cggcgtactg ggcgaggtct
     2221 tcgacgatct cctgcttgaa accacggtac tcgatggcgt catacatgcg gccgagcacg
     2281 cgggcggtgt ctttcatcga ctccttatgg ccgatctgcg aggaattggg gtcgatgtag
     2341 gtgacatttg ccccctggtc gtaggcggcc acctcgaagg cgcagcgggt acgggtggag
     2401 gtcttctcga agatcagcgc gatgttcttg cgctgcaggt gctgttgctc ggtgcctgtg
     2461 tacttggcgc gcttgaggtc gcgcgcgagg tcgagcaggt aacgcagctc gcgggcgctg
     2521 tggtgcatca ggctgagcag gttgcggttg tgcatattga aagccatggt tctttctcct
     2581 tgtgggcatc tgaaaagcac cctgcggcgg gtagccttga tcagtagtcg atggggtcgc
     2641 ggatgatcgg acaggtcata cagtggccgc cgccgcgccc gcggcccagt tcgctggcgc
     2701 tgatggtgat gacctccacg ccggctttgc gcagcagggt gttggtgtag gtgttgcggt
     2761 cgtagccgat tactacacct ggttccaggc agaccacgtt gttgccgtcg tcccactgct
     2821 cgcgctctgc ctcgtaggcg tcgccaccgg tttctactac gcgcagtttc ttcaggttga
     2881 gagattcggc gaccacgtcg aggaaggact tttcctcgcg gcgcacgtcg atgccgccgg
     2941 ggcggctctc gtccgggcgc aggatgaagg gcacgatgga gttggccacc tccgggaaga
     3001 tggtcaccag gtcgcggtcg cagaaactga atacggtatc caggtgcatg gctgcgcggg
     3061 agcggcccag gccggcgacc accaccttgt ctgctgcgcc cttggcgaac agcgcgcggg
     3121 ccacctggcc gatggcctgg tgtgaggtgc gctcgcccat gccgatcagt acggtgccgt
     3181 tgccgatggg catcacgtcg ccgccttcca gcgtcgctga accatggtct tggtccgggt
     3241 cgccgtacca gatatcgaac tcctcgttga cgaagtccgg gtggaatttg tagatggcgg
     3301 tggtcagcag ggtttcctgc cggcgcgccg gccagtgcat ggggttgagg gtgaccccac
     3361 cgtagatcca gcaggtggtg tcgcgggtga actgggtatt gggcagtggc ggtaagatga
     3421 aacttgactc gccgaggtag gcgttgaaca tcttcgctgc gtcggaatcc ttgtgtttgg
     3481 ccaggtctgc gccggagacg ccaccgatca ggaattcggc gatcctgcgc ggctccagcc
     3541 cttcgataaa cgaacgtacc tcgtcctgca ggccgaggcc gacgctgttg gggttgattt
     3601 tgcggtcgag aatccacttc agcgcttcgg ggttgcgcac tgtctcttcc agcaggttgt
     3661 gcatctccac cacctcgata ccgcgctcgc gcatcttggt catgaagtcg aagtggtcgc
     3721 gcttggcctg ggacacccag atcacgtcat cgaacagcag ctcgtcacag ttgttcgggg
     3781 tcaggcgcag gtgggccagg ccgggtgagc agaccaacac cttgtgtagt ttgccggctt
     3841 cggaatgaac gccgagggct ttcttggaca tagtgaactc cgtttcttca tagactcagg
     3901 gtgccctcat acagcccgta ggcggcgagg gcggcaccta tcagcacggc ggtgcagatg
     3961 atcttttcga cgccggtgaa gatgggttgt cccagctcat gcttggcctt ggcgaacagc
     4021 agggcaccag gggcatagag cagggcggat agcagcaggt actgcacgcc agcggcgtac
     4081 accagccaga ccgcatagag ggtcgagatc agcgcgatca acaggtcctt gttgcgctca
     4141 ccgcgggcgt tctcgtaggt ttcgccgcgc actgccagta gtaccgcgta agcgctggac
     4201 cagaagtaag gcaccagaat catggaagtg gcgaggtaca gcagcttcag gtaggtggca
     4261 tcgttgaaca gggtaatgat caggaacagt tggatcagtc cgttggacag ccacagtgca
     4321 ttgaccggta catggttgac gttctccttg cgcaggaact cgggcatggt gtggtccttg
     4381 gccgaagaga agatgatctc cgcgcacagt aaggtccacg acagcagcgc cccggccagc
     4441 gagacaatca gaccgatgct gatcagcatc gccccccacg gaccgaccac ctgctctagc
     4501 acggccgcca ttgatgggtt tttcaagcct gccagagcag cctgggtcat gatgccctga
     4561 gacagcaggt tgaccagtac cagcagcaac agcacaccga tgaagccaat caccgtggcc
     4621 ttgccgacat cgctgcgttt ctcggcgcgg gcagagaaga tgctcgcgcc ttcgatcccg
     4681 atgaagaccc agacggtgac tagcatcatg ttgcgcacct gatccatcac gctgcctagt
     4741 tcgctgttgc ccgcgcccca gaagtcgctg gtgaatacgt ccatcttgaa ggcgatggcg
     4801 gcgatcacaa tgaacagcgc cagcggcact atcttggcaa tggtggtgac ggtgttgata
     4861 aaggctgctt ccttgatgcc acgcagcacc aggaagtgca gcagccacag cagtaccgag
     4921 gcgcagatga tggccggcag ggtgttgcct tcaccgaata ccgggaagaa gtaaccaaga
     4981 gtggagaaca gcagcaccat gtagctgacg ttgccgatcc aggcactgat ccagtagccc
     5041 caggccgagg agaaacccat atagtcgcca aaaccggcct tggcgtaggc atacacgcca
     5101 ccgtcgagtt gtggcttgcg gttggccagg gtctggaaga cgaaggccag ggtcagcata
     5161 cccacaccgg tgatcagcca gccgatcagg gtggcgccgg cgctggcgct agcggcgatg
     5221 ttctgtggca gtgagaagat cccgccgccc accatggagc cgactaccaa ggcgactaat
     5281 gcgcctaggc gcagtttttg tgtcgaatca ggcatgccac atacctccat gttcaggggt
     5341 tagcaagggt agtgagcgcg tctgcgaggg cgaccgtatt gatttgaatc aacaggctgc
     5401 gattaattgc gcaaaggagt tttcatgaca ggctgctggc cgatcattta gctgaagccc
     5461 ttaggtttaa ggggttcctg acctcacccg gcaaatctgc gacaatcgcc accctcctgc
     5521 tcttcccctt gaccccaccc cgcgattacc cgatgaccga agccacgccc gccgttgacc
     5581 aactgctgaa aaacctcgac cagacgctgc tcggcgaccg tcatcgcctg cgtcgccagt
     5641 tgcatgaact gcgcaagcag agcccggtgg acgaggccaa gctggcgcag tggctggagc
     5701 gcttcgaggc gtcccgcgcc aaggtcgagg cgcgtcggct gagcgtgccg ccgatgcgtt
     5761 acgacgatgc gttgccgatt gccgccaagc gcgatgagat caaggccgcc atcagcaaac
     5821 atcaggtggt ggtgattgcc ggggaaaccg gttcgggcaa gaccacccag ctgccgaaga
     5881 tctgcctgga gctgggccgc ggcgtacatg gtttgatcgg ccacacccag ccacggcgcc
     5941 tggccgcgcg cagcgtggcc acccgggtgg cggaagaaat cggcacgccg ctgggcgagc
     6001 tggtcggcta tcaggtgcgt ttcgaggatc agagcaacga gcgcagcctg atcaagctga
     6061 tgaccgacgg catcctgctg gccgagactc agcacgaccg ctacctggaa aagtacgaca
     6121 cgatcatcgt cgacgaagcc cacgaacgca gcctgaatat cgacttcctg ctgggctttc
     6181 tgaaaaccct gctggtgcgc cgccccgacc tcaagctgat catcacctcg gcgaccatcg
     6241 atttgcagcg cttctccgag cactttggcg gtgcacctat tgtcgaagtg tccggccgta
     6301 ccttcccggt ggacacctgg taccggccgc tgacggccga gcaggacgaa gacggcaacc
     6361 gcgttgagga agacctgtcc gtcgatcagg cgctgctcgc cacgctggat gagatcagcg
     6421 ccttcgagaa agctgagggc aagcgccctg gcgatgtgct ggtatttctg cccggcgagc
     6481 gcgaaattcg cgacgcagcc gaggtgctgc gcaaggccaa cctgcgcctg accgaagtgc
     6541 ttccgctgta tgcgcggctg accccggccg agcagcagaa gattttcgcg cccatgggcg
     6601 cacgcaagat cgtcctcgcc accaacgttg ccgagacctc gctgaccgtg ccgggcatcc
     6661 gttatgtgat tgactccggc accgcgcgga tcagccgcta cagctaccgc gccaaggtgc
     6721 agcgtctgcc catcgaggcc gtgtcccagg ccagcgccaa ccagcgcaag ggccgttgcg
     6781 ggcgggtcga gccgggcatc tgcattcgcc tgtacagcga ggaagatttc aacggtcgtc
     6841 cggcctttac cgacccggaa attctgcgta ccaacctggc ggcggtgatc ctgcagatgc
     6901 tgcatctgcg cctcggcgag atcgaggcct tcccgtttat cgagccgccg gatggcaagg
     6961 ccatcagcga tggcttcaat ctgttgcagg agctctcggc ggttaaccgc gagggccagc
     7021 tgacgccgct ggggcgccag ttggcacgcc tgccggtgga cccacgcctg ggtcgtatgg
     7081 tgctggaagg ggccaagcag ggcagtctgg aagaagtcct gattatcgcc agcgccttgt
     7141 cggtgcagga tgtgcgcgag cgtccgtctg accgccagca ggccgccgac caggcccatg
     7201 cgcagtggaa ggatgtggat tccgacttcg ccgcgctgat caacctctgg cgcggtttcg
     7261 aggaacagcg ccaggccctg ggctccagcg cgctgcgtag ctggtgccgg aagaacttcc
     7321 ttaactacct caggctgcgc gagtggcgcg atgcgcatcg tcagctggtg ttgattaccc
     7381 gtgatctaca gctgtctgaa ggtggtcgcg gtcagggtag gagcggcctt ggccgcgatc
     7441 agcaatctgc aggtcaagcc gcgatagccg gggatcgcca gctatcggcg gcccagaaag
     7501 aaaaagagct ggccgagaaa gaccaggccg cccagcgcgc caaaggctac gccgccgtgc
     7561 ataaggcgat tctcgccggc ctgctcagcc agatcggcca gaaaaccgaa gacggtgact
     7621 atctcggtgc gcgtcagcgg cgcttctggg tgcacccgtc ctcggtgatc ggtcgtaaga
     7681 aacccagctg gctgatggcc gccgagctgg tggaaaccac caagctgttt gcgcgcatgg
     7741 tggccaagat cgagccggac tggatcgagc cgctggccgg ccacctgatc aagaaaaacc
     7801 acctggagcc gcactgggag aagaaacgcg gccaggttgt agccttcgag cagatcagcc
     7861 tctacggcct gatcgtggtc ggccgccgcc cggtgcattt cggccccatc gaaccggtgg
     7921 tctgccgcga gctgtttatc cgcgagggcc tggtcggtgg cgagatcaac tcgcgcgcca
     7981 agtgcctgac cgccaaccgc cagctgctgg aaaagctcga cgagctggaa gccaaggcgc
     8041 gccgccgcga cattctggcc gacgatgaaa ccctgttcgc ttactacgag gcgcgcctgc
     8101 cggcggatat ttaccagagc gcgaccttcg acagttggta caagaccgaa agcgcgaaga
     8161 acccacagct gctgatcatg cgcgaagagg acgtgctcgc tcgcgatgcc aaggaagtta
     8221 ccgccgcgca gtacccggac agcctgcgcc tggacgaact gaccctgccg ctgagctatc
     8281 actttgagcc caatcacccg cgtgacggcg tgaccctgcg cgtacccgcg ccgttgttgc
     8341 cgcaactgcc gcccgagcgc ctggagtggc tggtgccggg cctgctggag gccaaatgca
     8401 tggccctggt gcgcggccta cccaaggcgc tgcgcaagaa ctttgtgccg gtgccggact
     8461 ttgttggcgc agccctgggc aagatcagct ttgccgacgg cagcctgccc gaagccctgg
     8521 gccgtgagct aacgcgcatg accggtacgc gggtcagcga ggaagcttgg accgagtctg
     8581 cgtcccagat tgaacgccat ctgcgcatga atatcgaagt ggtcgacaac cagggcaaac
     8641 ccttgggcga aggccgcgat ctggccgagt tgaccgcgcg ctttagcgaa gccacccagg
     8701 ccggcctggc cctgccgaaa accgccaaga gccagcggcc ggtggaggcc aaggccttta
     8761 gcgaagtggc ggccaaaact cagcagcagg tcgctggatt atccatgacc gtctacccgg
     8821 cgctggtgga agaggcgggc gtggtaaagg aagggcgctt cccgaccctg gccgaagccg
     8881 aatttcagca tcgccgtgct ttgcagcgtt tgctgctgca acagttggct gagcaggcca
     8941 agtttctgcg cagcaagctg ccggggctga ccgagctggc gctgctgtat cgcgagctgg
     9001 gccgggtcga tgcgctggtc gaagatattc tgctggccag cctcgacagt tgcatccttg
     9061 aaggcagtga gccgctaccg cgtgacggtg ccggtctggc tgccctggcc gagcgcaagc
     9121 gtggcgactg gaccggcgaa gccgaacgcc tggccaagct gaccttggaa attctcaagc
     9181 tgtggcacgg tctgcagaaa cgcttcaaag gcaaaatcga cctagcccag gccatggcgc
     9241 tcaacgatat caagctgcag ctgagcaatc tggtgtatcc cggtttcgtc cgtgaaactc
     9301 cgacgcagtg gcttaaagaa gtgccgcgct acctcaaggc cattgagcag cgcctggaca
     9361 agatcgccgg tcaggttcag cgcgaccgag tgtggagcgg cgagctggcc ggttactggg
     9421 agcagtacca ggcgcgcctg ggaaaacaca gccaggaagg caaacgcgac ccgcagctcg
     9481 tgctgtatcg ctggctgctg gaggagtacc gcgtctcgct gttcgcccag cagctgggca
     9541 ccaaattgcc ggtctccgac aagcgcctga gtaaacagtg gagcagcgtc gaaggctgag
     9601 gaagcagtgc atccatacaa cagctaactg ggacctagcc ttaaatagcg actgcctaca
     9661 aaaaccaaac gggattggcg ctttgcacgc ccatcccgtt tttgctttaa gccatgcagc
     9721 aaagacccag ccggataggg actaagccac caccgggatc aatgggtcgg ccgccgccag
     9781 ggcgatcgcc cgatccgctg ccggtgggta gatccactgg gtgttgatct gggttttcag
     9841 caccttgcct tcctggccgg tcagcagcac cttgcgctcc cagccttcgg taaataccgc
     9901 gccccatgca cccagatcca ggcaggtgac gtacttgtgc tggctatagg caatcggcgc
     9961 gacaccgagc agatcagcgg cggcgttgtt gccggccgag cggcccaggg caatggcgtg
    10021 ttggcaggtc atcagggcgt gattgccctg gtcatccacg gcggcgtagg cggtgtcgcc
    10081 gctggcgtag atggcatcct ggccgagcac cttgaggttg cggtccacgt gaaggcggcc
    10141 atgcgcgtcg cgttcgccgt taatctgttg ggtcagtggc gaggctttca cacccacggc
    10201 aaagatcacc gtgctggcgg caatgcgttc gccgttaccc agctgcacgc cgtgctcatc
    10261 gaccttggac accgggctgc cgagccgcca ggtgacgccc aggctatcgc tggcttcggc
    10321 gacgatcccg gcgattgcct cgcccatggc accgccaacc tgcttggcca tatccaccac
    10381 gatcacgcgg atgtcggtct gctcaccgag gatggcacgc aggcgtgccg gcatttcggt
    10441 ggcggtttcg ataccggtaa agccgccgcc gaccaccacc acggtgttgc gtgctgcgct
    10501 ctctggctgc tgcgccaggc tttgcagatg ggcttcgagt cgctgcgccg actccaggct
    10561 gtcgacatcg aaggtaaagc gttcgatacc ttccagtggc gggcgtgcca cctggctgcc
    10621 ggacgcgagg atcaggcggt catagttcag ctcggcctcg gcacccgagg cgtcgcgata
    10681 gctgacgcgt tggctcgcgg cattaatctg cgtggcactg ccctggacga agcgcacgcc
    10741 cacggcctcg aacagcgggc cgagcggggc gacagtgctg gccaggtcgg tttcataaaa
    10801 gcgtgggcgt acacgcagtt ccgcctgggg cgcgagcagg gtgacgttga cgtccaggcg
    10861 ctgctgttga tcgagcaagc gcacggcgct gagtgcactc cacatgccgg caaagccggc
    10921 accgatgatc aggatttgct gtttcatatg ggtaggttcc cgtagtgaag agaaaggctg
    10981 ctggctaacg acgggagtga ttctgcctct ggcttggatc aaggtattga tccattattg
    11041 cctgaactca ttggtccact ttggagcggc catgcccccc gagaaatcgc caccgcaggt
    11101 agtcttcctg ccattgggca cgcccgagcc tggcgtcagc ctgcagcgct ggctgtatga
    11161 ggcgctgcgc ggcgccattc tcggtggccg cctgccggct ggcagcaagt tgcccagcac
    11221 gcgcgccctg gccgagcact acaagctggc gcggggcact gcccaggcgg cctatcagca
    11281 gttgttgtcc gagggctacc tggaggcgca gaccggcagc ggtacgcggg tttgccaggt
    11341 gttgccggat gccacgctga acgccggcta tgtgcgacgc aaggttgtgg cgactgtgga
    11401 tgtaccgcgt aagccgccga caacaccctg gatcgagcgg ttggaggcga ttaacccggt
    11461 gtttgcgcgt cgcccggtaa tgccagggtt gcggccgttt ttcccgcatc ggggcgacac
    11521 tcaggccttc ccggttgacc tgtggcgcaa gctgcatatc cagcagctgc gtgcgtcgcg
    11581 cctgtccatc ctgctcgatt cagaccctgg cggcttgccg gccctgcgtg aagccatcgc
    11641 cggctacctg gccatcgctc gcggtgtgca ggtgcccgct gagcgcattc tggtcctcaa
    11701 tagcgtgcag caaggccttg atctgtgcct gcggctgtta gcaccgggca attcccaggt
    11761 atggatggag gaccccggct atccgggggc gcgccagctg atcgatgtgt ccggtgtgcg
    11821 gcgggtggat gtgccggtgg acgccttcgg tattcaagtg gaggagggcg tgcgtctggc
    11881 ccccgatgcc cggctggcct acgtcacccc ttcacggcag gcgccgctgg gcggtgagtt
    11941 gtcccctgcg cggcgtctgg cactgttgca gtgggcggcg cagcacggca gctatatctt
    12001 cgaggacgac tacgacagcg aataccgctt tatcgccaag ccgattccgg cgctgcgctc
    12061 gatgccggca gcggagcagt cggtgatcct cgccggcacc ttcagcaagc tgctgttccc
    12121 ctccatccgc ctggcctacc tggtgttgcc ggcgcacctg cttgagagtt tcacccgcgc
    12181 cgccgcgctg atgtcacgcc acgccaacag cctgtcccag gcggtgctgg ccgactttat
    12241 ccatgaaggg catttcgacc gccacgtgcg gcgcatgcgc aagatatacg cctcacgcgc
    12301 cgaggccttc gccgagtcgg ctcagcggca ttggcgaggg ttgatcgacg taccggagat
    12361 tcgtgcaggg ctggatatca cctgcaacct gcaggtggac aacgagcagg ctgccttcgc
    12421 ccgtctgcag gccgccggta tcgacaccat gccgctggcg cgctactgcg tgcagccccg
    12481 aaaaccgggg ctggtgctgg gctttgcgcc ttacgatgag cgcgctattg aggatgctgc
    12541 caaggctgtt gcgctggcct tgaagcaata aggccgcgcg ttctgacgca gccctgggtt
    12601 gtaaccgagc agttgggctg ccggttggcc acagaaatcg gccagcagcg cttaaacaaa
    12661 tacctgccga gccatcccgc gcgggatgcg atttttaccc ggcagttgtt ccaggcgttg
    12721 ctgccattcg gctcggggtt tcagggtagt gcttggcttt accatcgcag ccgctgcggc
    12781 agcggccttt gcagctttgg cagcggctct gagttcagca agacttgggg ttttacggac
    12841 aggttcggca gtgccttttt caacgctgcg caggcacagc ttgcatgaaa cctgagtgac
    12901 atcctgggtg ctgctcaggt tcgagccggt gcgtccacat gcgacatttt ccgcggacgc
    12961 ggtgtagtga atagccaacg tgttgtctcc tgcattttcg gggcgcggga ttctacacgg
    13021 ttcgtttagc acgccaactg cttatcgttg cttccaccaa gcacgtcgcc aggcgtcgct
    13081 ggcgcatcgg tcgtgcgacg cgcaaagagt ctgaactcat tattgcgccg gccagtcaga
    13141 cacgaacatg ggcgggacca cctcccacgc tcgtgaacgt aaaaagtctc aaggagaata
    13201 accatggctc gcaagacgat tgacgacctg ttcgtccatg aactttccga tatgtacagc
    13261 gccgagaagc agatcagcaa agcgctgcca cgcatggctc gcgccgccag caacccgttg
    13321 ctggccgagg cattcaccgc gcacctggaa gagacgcaag ggcagatcga gcgaatcgat
    13381 cagctggtcg aactggccgg gctgaagctc aaacgcatga agtgtgtggc gatggagggg
    13441 ctcgttgaag agtccaagga actgattgaa gagatcgata aaggtccggt gctcgatgcg
    13501 ggtctgatcg gcgccgcgca gaaggtcgag cactatgaaa tcgccagcta tggcacgctg
    13561 atcgccatgg ccaagcacat cggcataaaa gcagccgcca ttcagttgct caaggacacc
    13621 ctggctgagg aaaaagccac cgatgagaaa ctaaccaaga tcgccgaaca aggcggtaac
    13681 caggcagcca ccatgaactc ctgacgccgt gtgccgttgc cgacaggtgc tcttgttggc
    13741 agcggctcac ccagcttgcc gcgcagcgct gcttctggca ccgagcgctg ctgtagccct
    13801 gtatcgcggt cgttgaacgt gtccagaatc atctcccccg aaagcgccat cgtcgaggca
    13861 agcgaagcat ccgatccgct gatgttcggt acgccgagag agcggctgga tttctaccga
    13921 cgggaaatcc agtacgaaac cagcctgttg gccaatcgaa ccaatgccta cctgtcggcg
    13981 caatcgttcc tggtgatcgc ctacgcctct tccatggcca acgccaatac gcagtggggc
    14041 gacctgttta ccctggtggt gccggcgctg ttggcgttgt tgggtatcgt cagttcactg
    14101 cacgcctggc ctggtatcaa ggcttcggca gatatcatcg gccactggca tttcaagcag
    14161 gaccacctgc tgcgcagtga accaaaattc ggccacgcct acgacgactc ccccctgttc
    14221 tgtgagcagg agtcgaacaa ggacagctat tacaagtccc tgttgttctc gctgcgctca
    14281 ccctggctgt tcgccgtctt ctggggagtg ctgggggcgt tttcgctgtg gttgcagctg
    14341 agcgcggtga agctctgacg gcctgcgatc aggcggcggg cagctgtcag tgcaaccgcg
    14401 aatgccgcag cgcgggcgac cagctgccgt gctcacagcg agcgcagagg ctgtgctgct
    14461 ggccttccat ccactgcgtg ttcccgcaac ggatacaggc atagagatca cttacaggca
    14521 cgcgctcata caccgcccgg tgttcgggcg gcagtaccga cagcccaggt ctggtttcct
    14581 cggtggcgta gaaatagagg ctgatatcgc catcggtttc aaggataccg aggcggactt
    14641 ggccgaggtg gtcgacgctc tgctggcgca gctccatgaa aaattcatcg gaagaaatat
    14701 gcagacggtc caggctgttc agctcgtaca gcccatcctt gatgatcgtg actggctcgc
    14761 cttccatcca cttcgaaagc cgtgggctac ggctcatcga tagaacagtg atgcggtaaa
    14821 gcagcagcaa cgttgcgaat accaccagca ctggcagcag cggtacatcg tcgtaaaacg
    14881 acacatcacc cgccgccgag cccagcgtca gaatcatcac cagctcgaac agcgacaact
    14941 ggcggatgcc acggcgaccg ctgaccttga gaaacagaaa cacgacaacg aacgcgaaga
    15001 gggcgcgaag cgcgacctcg cccaagaaca gcacgggaaa atcatccatc agcatgcgtt
    15061 gccaatcgaa agcggtcatc cacggctccc ctgagcgatt cacttaccgt tgagaccctg
    15121 gcatcagcga atggatcagt ttttaagcag gcgccagtct ccgccaggca gtcgccatat
    15181 tgcccccgca cacaaaccgt ccatcaaact ccctgaactt gcaccggctc ttgcgcttct
    15241 gaacctgtgt tatccacccg caaaggagaa acagtcatgg ctacccctac cgagacccgc
    15301 tcggcagcgc ccggcgtaga agccaaacgc tgagcgagga gagcaccgga gctattcggc
    15361 gctaatgcgg atgctaaaga ccctgacctt tgccgttctg catttctcga ttgccttcac
    15421 agtggcctat atgctcaccg gggacttgct caccggtggg ctgatagccc tgatagaacc
    15481 cgcctgtaac acagtggctt attacttcca tgaaaaagcc tggctgcgct tcggcagcag
    15541 ctcgtcggcc gagcatgacg gctgcggtca cggcggcctt ctacgctttt tcaaacggga
    15601 ctgagtcgcc agcggcggat caactaatcg gtttgggaat cagacaaaaa agccccgcgt
    15661 taataggcgg ggcttttact tgaagcagtg ctcagagctg tggggaaggc cgggtagtgg
    15721 cgccatatcc aagccttgca tggttgtacg ttacgaccct attgccctgg gctttcactt
    15781 cgccgcgaca gttgttgggc ggaaggtgtc ggttgtcggt aatcggcaat atgtccggat
    15841 tcgctccaag cattagggat agaactggct tggagacggc aggggggctg gataaagcta
    15901 tttgaccagg gcgggttggt agctgaatgc gcaatagagg gctgttggtc agtggggcat
    15961 tacaatttta tcttcggata gtctcgtatt gcgagattta ttgatttcag tatgtgtgag
    16021 aaacgcgaga aattaataat tttctgatta ggtttaacca cgtgttgaga gaattgatgt
    16081 ttgctatatg tggtgccttt aggtccaggg cttaccgctt caaagctctt tccgttataa
    16141 gttgctcgta aaactgctgg tgggaaaaag tcttccatca tggagtcttt tccgtttaat
    16201 agaggggtca atactacata taaattttgc gccaccttgt agagtgtttg gcttttatcg
    16261 aaaatcttcc ctttgccact tggaatggag tatcggtcac cagtcacatc ctttatagcc
    16321 tggaatattg gctgagatcc actgtcgtta tcaaccacta taatagtcgg gttgagtggt
    16381 ggatgtgctt tgtaactgcc gcactgttta gcatacctcc gtatgagatg accaagatca
    16441 gagctgcctc cattcagccc cattactcga tgcgtcagtt cggagtagtt aataaatcga
    16501 accttcaact taacttttcc agacgcgtca ggcttggcta atctaggaaa tttttcgtgc
    16561 agagatttta ttgcgcaggt caaatagata ttatcagttt ttccttcgca gataattgtc
    16621 ggggctggag atgcatagaa gtttttgtaa aacaaaaagt ccccgtgcaa tttttcaaga
    16681 gaggacagtg ggaagtcttc atgcctgcga ttgctatttt caatgatttt tttccgatta
    16741 aagcagtcgg tcatatatat gtagctatac atgccttcaa gtttgtttag tttgctatgg
    16801 tttaattcgg tttttttggg gcttcttggt ccgttttctt catggacata gtactccccg
    16861 tccataaaaa gcttgtgagt catagctctg acttttcgcc taaattctgc cggtgtattt
    16921 gtgctctgat tgacgacgag gcccgtaacc gtttgttgcg cccgggcgta ctgcattcga
    16981 gtcttttcat ggttcaaccc aaagccgctt ttatagatta ctttcaggag tgcgccgcct
    17041 ggtatccagt catggctatt ttcaaggcga taagcaattt cgggtgggaa ttcagacttg
    17101 tttgttgaga atgttatgtc gtccgcgtaa cgtgagtagg tgcagcctag cctcttagcg
    17161 agctgcacca tacgaatatc aatcaagtgc ccaatcaaat ttgatgctac aggcgatgcg
    17221 ggactgcctt gagggagttt gtcgtcgtga caaatgatct gcgccaagat tgttgcagtc
    17281 ttggtgttta aattaaagtc tttattacta ataaaaaatc cgcgaattct ccccatgtgt
    17341 attgagtcga aaaagccttt gatatcgaca ttaaatacat atagccgctt tctgtggggt
    17401 tctgcattcg ttatgattga gtggttggcc ttaaatccat gagaaagtga gcctaaagga
    17461 gatcgcttct tctcgatgct ttccaaacat tcatctaaaa gtttttttag cttccgttgc
    17521 aatgacttga gctcatcgca tggcgctaat atttctcggg gagttccgtt tttcttgggg
    17581 atttggaagc ggctatattt gccggttttt ttatataata aatatgcaag agcagtaggt
    17641 gagtagttaa gaattactgc gagatcatga agcgtcgtgg ctttttggag tgcgcgtaat
    17701 cgaggcataa cctatccctg gtaaaagaga gacttacagg cactcatatg cgtccgtcag
    17761 gatattcata tcggtccatt gtgagtcgcg aaggagcaat atccagcacg attatttcaa
    17821 ataccacgaa ccgtggtaaa aaatctgcct gtaagtctct actcgaactt accataaata
    17881 tcttgatctg cactacccct gtatgcctac cccctcatga ttttaagctc tgacgagcct
    17941 cagaaataaa tcgcgaagct ctgcattaag cgttcgttgt cggacgattc ccgcaggttc
    18001 gcaccgcagt tttggggcga tgtagttgaa aaaaatacct tcaattcctg tctacaaaaa
    18061 gagcgtgcct acgttgaaat ctgagcaggg ggagagatga actgccttga gtttcacggt
    18121 gcaatgtcaa atatgctgcg gttttgaact ctggaaaggc aggttcgggc agacctactt
    18181 ttcaacagag tcgaccgaaa gctgcccatt agtaaagctc gcaggcagag ctccccctaa
    18241 accccaggcc cgagtcgggc acacatcgct ggtcgccgcg atttgccatg cacggcgtag
    18301 ctcgacagaa tcgcgccccg attcggccca acggccggac gtcgatgctg agaaggggct
    18361 ggagttgaac gagcagacat tgtctatgcg cctggaacgt gtggcggtgc atgtgccggc
    18421 aggggcgcgg ctggctgata ttggctcgga tcacgcctat ctgccggtgg cattgctgcg
    18481 tcgtggtgcc attgaggcgg cagtggcggg ggaggtggct ttgacgccgt tccacgcggc
    18541 cgaacgtagc gtgcgtgaaa gcgacctggg ccaacgcatc aatgtgcgcc tggcagatgg
    18601 tttggcggcg attgaagcgg gcgatgcaat cacggcaatc agtatctgcg ggatgggcgg
    18661 cgagcggatt cgcgacatcc tcgacagcgg taaggcccgc ctgagtggtc aggagcgttt
    18721 gatcctgcag cccaacggcg gcgagcagcc attgcggcaa tggctgatgg aaaatggcta
    18781 ccgcatcctc tgcgaggagg tgctgcggga gaatcgcttt gtctacgaaa tcatcgtcgc
    18841 cgagcgctct ggtccggtga cctacagcgc cgaagaactg tactttggcc cgctgcagat
    18901 gcaaacacgc agcccggcgt tcctggccaa gtggcagcga atgctgcgtc tgaagcagca
    18961 gaccctggcc gacttcgctc atgcgcggca ggcggtgtgt gaggagaagg tgcagaaggt
    19021 cgcccaacag atgcagtgga ttactgaact gttggcttga gcctggttcg gcaggcgagg
    19081 gcgctaaagg aggggctcga tctcggagcg cacggtgtag gttgagacgt tattgccttg
    19141 gctcatgagt ggttacttct ggacgattgc agtatgtcgt taagggcggc tgtcgaccca
    19201 aagcgggcag ttgcgaagcg cgggagtcgg gcagaagcgg tcatcgagtg cctacaagac
    19261 cagcggcgat tcactggccg caaacaagtc ttggctttcc tttccatgag aaagattacg
    19321 gcgttgtgtt tttttgcttg accttgccac gatggcaagg aggaaagtcg aatcatcaaa
    19381 cactaccccc agtaggagcg agcgatgact accttcgaag taaaagacat gacctgcgga
    19441 cactgcataa aagccattac ccaggctgta ctgactgtgg acccgaccgc caaggtgcaa
    19501 atagacctat cttctcaccg agtccagatt gagtcggcaa gcgcagaagc cgaactcagc
    19561 catgcaattg tgaaagcggg atactcccca atcacgaaaa cggccgagca agcttctttc
    19621 gcgccgatca agacgacagc ccgcgtttgc tgctgccatt gaatgccacg caagctcaag
    19681 acaaggtgcc cccgcagcct taatgcggcg aaggcaacaa aaaacccgcg aaagcgggtt
    19741 tttcaagacc attccgacat cctgtctgta gccatccaag ccatggttga tcatccgtaa
    19801 ctctgagcgc gttctgcact cggttgatgt aactcgatta ggttgaacag caagttcctc
    19861 acagacgcga ccggcggcgt ttggccgatt tcagcctgtc gcgataggcc agagtcggcc
    19921 agaagctgcc cttcatagcg agcagcctat acatgtttaa aaaaatcatg accctgctcg
    19981 ggaaccaccg tgtaagttcc tgttgcccca gctttgcagt gcatgctgcg taatactcga
    20041 tattctccag cagtcatctg ctctgctgag caaaaaatca tagttgccgc aggaatggaa
    20101 gaagttttca ccgccaatat cggtcgattg agaatcaccg aatcgtagga tcaagtaatt
    20161 gcagcgcgcg ctcaccgagt cttcattctg ttcatacgcc aggagaagat tcgaaaagct
    20221 atggtgtttg gccaagttgt cgacagcatt ctctcggcgc ttaacgaagt ccttgccgct
    20281 catagaggat ggtttttctc tgccggacga agagtaatca atgaacacga gcgaggatag
    20341 gcagttggtc atcgaggccc agtcccgacg gtcgaaggaa aggaaaaatc gacacaggac
    20401 gtcgttaatc gctacaaaat cgtttgggct gactgctttc atagtttctc cgggtatcac
    20461 atagccgtat gtgctagggc attttcagtg ctaatccaag cggctactgt gagagctatg
    20521 caagcggccc acagtgatcc gttgaagttt tgattcatcg tgtagaaaac gtccgctaca
    20581 caggggccag caatttggcc gacgctgtag ataagcggcc agacagtggg cgtggtgaca
    20641 gcggctcagc cgcttcatgc gaagcatcag gctatgcgcc agcgaggaca agcacgacgg
    20701 aatcagacaa agccgaaagc gcttatacag gacaaggccg tcgataccgc tgcggaccag
    20761 attgtggatt aaaatcactc gcgctcggca ccgagccgct caattactga caagttgcgc
    20821 gaggtgatga tgaggcggct cggagagctg ccggtaccac ctttcagata tgtccgctat
    20881 ggccgaaatc ggacatctag aacagaaaaa atctgccacc tttgtccgac gcagtcagcc
    20941 agactgaata cactatgcgc ccgactaccc tgggccataa atatggctgc ctcgaacaag
    21001 gaactcgcct ccatggcctc cccagataaa cagcaaaaac gcgcccagcg ggccaaagcc
    21061 aaggccaagc aaaaccgttc gggcaagtcc aaggccaatg tcgtccatcc gctgttggcc
    21121 aatccgctga tcaatgagcc gttcgatgat gttgaaattg atctgagcac cttcgacttc
    21181 aaagacatcg aagagaacgg attcgacccc gcgcacttcg atgacctgtt ccaggcgatg
    21241 aaggtgggtg aaggcatcag cctgctggcg atgtgcttgg tgtttctgca atacccggtg
    21301 ttggagctgg tggtcgcaga agaggcggaa gaggccgcga ccgactttat gatgggcctg
    21361 ctgatcgtct atcgcggggt tttccacgat gaagacgagg acgctgcggt gacctggatc
    21421 ggcagcgacg ccttccagca tgcttacaac gaagcgtcga tgatcctgca gaagaagaac
    21481 gctcgcagca gcgcccgcgc gtagaaagcg cgatctcccc agcggctttg gtttcgatcc
    21541 ttgataacca tcagccgctg tcacaggttg gtcgcgtctg gcgaagtgtt cgggttttgt
    21601 gtcgggctag gcacctggcg ctaggcggtc ggtaccgttc cgccatcaat cacaaattca
    21661 ctgccggtga tggaggccgc acgtggcgag gccaggaagg caatcaggtc agcgacctct
    21721 gccggcttgg caggccggcc cagcggaatc ccgccgaggg catccatgat gatgcgttgg
    21781 ccgcctgcgt agtccgtacc ggcttcctgc gccagccgct cggccaaggc gaccgacgct
    21841 tcggtttcga tccaacccgg cgatacccgt acaacacgca cgccttgtgg cgagacttct
    21901 ttcgacaggc ttttgctgta ggtcgacagc gccgccttgg ctgctgcgta agccgtagtg
    21961 gcttcgggca agggcagacg gttttgaatg gaggtcacat ggatgatcac gccgctcttg
    22021 cgctccagca tctgcgggag cagggcgcga tccaggcgca cggccggcag caggttgagg
    22081 tcgagttcgc gttgccactg cgcgtcgtcc aataccgcaa agccgccgct gggtgccgac
    22141 gaaccaccca ggacgtggac gatgatgtcc acatcgccca gccgttgctt ggctgcggct
    22201 accagtgttt cgcagccttc cggggtcgag aggtcggcct gcacgaacag ctcagccggc
    22261 agatcgctgc tggcctgccg ggcggaagtc agcacttgtg cgccctcggc gaggaatagc
    22321 tcgaccaccg cgcggccaac gcctttggtg ccgccggtga ccagcactcg cttaccgctc
    22381 agatcgaggt tcatggcgca atctccagtg agcggatctg gccgtcctgc agcaggaaca
    22441 gatgattcag ctgcagcggg ctgccaggga agttaccgac caggcgcgcc gtgacgctca
    22501 gcgtgtcgtc tttctgtgtc gccgccagcg gctctaggct gtaggtgaat gcctggcggg
    22561 cctcctgttg ccaggctttg atggctgcaa tgccctggtg cgtgcggttt tcgtcggtca
    22621 ccttggcgtc cgcgtagaag caggcggcaa ggcgcgagat gtcgccacca ttgctgacat
    22681 cgaagtaggt ttgtacggcg tcaggcagtt gcaggttcat ggcctgatct cctctcacag
    22741 tgggttgggt ttacgactgt gatcttgccg cttggttgat agtttgctca actgggatag
    22801 aggttaacga cctatcccga atatcaggac aattgcatgg cagattcgcg ccttggtctc
    22861 aatgagctgg aagctgtact ggccgtggcg cggcgggcgt cgttccggca ggcagccatc
    22921 gatctggatg tttcgaccac ggcactgagc aacaccattg ccaagctgga ggcgagcctc
    22981 gaaacgcggc tgttcaatcg caccacccgc agcgtgtcgc tgaccgaggc gggcgcgatg
    23041 tacctggagc aggtcggccc ggcgttgcag gatgtacggg cggcgcttga agcggtgcgc
    23101 tcgcataatg tcggaccgtc gggtgtgttg cgcatcaatg cattcgccag tgccgcccgc
    23161 gctgcgctgg tgccgttggt gctggagttt ctgcagcgtt acccgaaggt gcatatcgat
    23221 ctggtgaccg aaggccgcct ggtggacata gtcgcggatg gtttcgacct gggcgtacgc
    23281 gctgagaatc ttgtgcccag cgacatgatc gtgatcccgc tcggccagcc gcaacgctac
    23341 gctgtggtcg gctcgcccgc gtacttcgcc cagcacggtc ggccgcgtag cccagcggac
    23401 ctgctccagc atcagtgcat gcgcatccgt ctgcccaatg gggcgatcta cccctggcat
    23461 ttcgagcgcg cgggcgaggt gactcggctt gatgtgactg gccagctcac cctggacgag
    23521 ccgagtgtcg ccaaggcggt ggtgcaggca ggcgtggggt tgggcttttt tatgcagcag
    23581 gacgttcagg ctgagctcga cgccggacag ttcgagcggg tgctggaaga gtggacgccg
    23641 cccagagaca acctgtgcct gtactatccg aaccgacgta acccgtccgc tgcgctcaag
    23701 gcattcactg ccctggcacg cggcaatctg ccgagtaaag gtctgccggg ttaattcccc
    23761 ccggctgtcc acaaacgcgt ggtatcaggc gatcaagccc agggtcagcc ccttcaaggt
    23821 ggcggccgcg cgggtggagc aacccagttt gcggaaaatg ctttccatat gggtgcgcac
    23881 ggtactcggg ctgatttcca aacggcgcgc cacgtccttg ttactggcgc cgcggctgat
    23941 ttccaggagg atggagcact cgcgggttgt gagcagcgcg cttttcggct gaggcaaccg
    24001 gcgttcgccg cgcgcggctg cgatcaccgc ctcgcagatg tcgccgtcaa acagaccgcg
    24061 gctggcgtcc tgtttgagca gccgcgccgc ctccgattcg ctatgggcgg gccgccaggg
    24121 cctggcactc gttaacgcca cccaggcgac agcgctggcg agcaagcgat acgcggcgca
    24181 ctgggcatcg gccgacgtgc cgcgaaagta gccgctgccg tcgagccgtt cgtaggcatg
    24241 ggcggcaatc tcgccaggga tgatcagctc actgatctgt cgtgatgcct tatgcgtcca
    24301 gtaggggacg aggtggatgc gctctgtggc gccgtagggg agcggccccg cggtgttcca
    24361 cagctgattg gagacggttg cccggcccag gccatggatc agggccgcct tgccaatctc
    24421 catctgggct ggctcgggca gcccccacaa gcgggcggcc tcgacggcaa tctgcgccac
    24481 ctgccgggaa taacctgcga gccagggcag cttcagatcg atcacatcgc ccaccagtgt
    24541 cagtgacacg gggcgttcta gccgggtgtc gtcctgcacg tcgggcgttt tttccagttc
    24601 cgccaaccac tccttggcat tgcgcaccaa cattgaggca agaacagcgg ggtagcggcg
    24661 gttggcctgt tcgctgatcc agctcagcgc cgcatccagg ccatgggcgc gacagaggat
    24721 gtccaggtcc ccggccagca ccacctggta ggtcacgtcc gggatgtact catggcttac
    24781 gcctaaaggg cggccggtgc cgtcataggt ttcgaatatc cggcacaagc cggcttcgac
    24841 cgccgcgcct agttcgaggg ttttagcgat ctggccagac acctcgcaat gcaccacggc
    24901 gagtggagcc gcctggtgca cggcattcat ctgttcagcg cccagggtct ggtcgagcat
    24961 cgcgcggcgg ccatcgacat cattgcccag caggtgggcg aagccatcgg cattggcggt
    25021 gcagccggac cagcgcagca aggcgacttg ctgcgccgtc gtcagatggt cgccgtcgcc
    25081 gtgcgtggcg cgggccagca tttgcgccag gcgggcaacg cgctgggagt ggccaagggg
    25141 ctggcccatg ctgaggtcgc cgacctggga gatagccgcc agtgccccgc tcaatgagat
    25201 gtcgaatcgc aacgcgctat ccataagaaa aagcccctgg gtcggataaa tgaccgatac
    25261 ccgccaagca tgtgggtccg cagaatttga actcccaaca caggagttca ccccatgaaa
    25321 acattcaagt cgcaactcgt caccaccgcc ttcgccctgg cgagcacctt cgtggtgagt
    25381 gtcagccagg ctgccgaggc aacgccttcg gtggtcatcg tacacggcgc cttcgccgac
    25441 ggctctgact gggccaaggt ggtgccgtat ctgcaggccg aaggggttgc cgtgacagtc
    25501 gtacaaaacc cgctcacttc gcttgctgat gacgttgccg caacgcaacg cgtgctgaac
    25561 aatcagtcgg gcaacgtggt gctggtcggg cattcatggg gcggcacggt gatcagtgaa
    25621 gccggcgccc atgagaaagt cagcgctctg gtgtacatcg cagctttcgc acccgacgcc
    25681 gggcaattca ctggcgagca gggcaagggc gcgcctacgc cgcccggcat cagccagatc
    25741 aaggcagaca gcaacggttt cctctacctg accccggagg gcatggccaa ggacttcgcc
    25801 caggatctcc cggcggcaca aaccgcggtg atgagcgcaa cgcaaggccc gatcaaggca
    25861 tcggccttcg atgagcggac cacggtcgct gcctggaaga ccaagccgtc ctggtacctg
    25921 ctcgcctctg aggaccgcat gatccacccc gacgttcagc gctccagtgc caagcgtatc
    25981 ggcgccaccc tgaccgaact gggcaccagc catgtgccgc agcagtcaca gccggctgag
    26041 gtggccaagg tgattcttga ggcggtacgc agtgttgatg cgcagtgatt gctgcctact
    26101 gatacgccgg ggtttgtgat gcctgcacga cgggttgcgt ggttgcagcc cgtctggtcg
    26161 acccgccaag tatgagtaac ggcgcgtact gatctgcctt gtaaaccgac actaacttta
    26221 acgacatcaa ggcagggatg cttcgagacc cctctaccgt tcattgcctg acggcacagg
    26281 caatgctgcg gtcatctgag cgtggcaggc cttgagtatt tcatcggcga gcggcgagtc
    26341 atccgggcat gcacgtgaca acatgatcgc gccgacagcc tgcgccagca tgtcgatcac
    26401 cttcacccgc gcgtcccccg gtgctgaatc tggggctgtt gggtactttt ccccgagcag
    26461 ctgcagggtg cgctctatcc cttcggcaaa cgccaccttc aattcgtccg gctgtcgcgc
    26521 agcgtcgcca cacaacgccg ccatggtgca accgctgccg cgcccgtcgc ggtgatcccg
    26581 agtcacgtac agatcgataa agccctgaac atcgagcgct tcggcactgg ccagcgagcg
    26641 ggaaaggctg caggcagagg cctcagccat cagatcagcc tttgagccga aatgcttgta
    26701 gaagccgccg tgggtaaatc cggctgctgc catcaggtcg gccacgccga caccgtcaaa
    26761 gcctcgctcg cgaaataccg cagacgcggt ttcgacaatg tgctcccgat ttgcctgggc
    26821 ctgagccttg ctcactctca cgtgtgacac ctcgtgtgcg ttcaaaaaac atgcgccgat
    26881 aatacataga tgtcgatcat aatcaaaatc gttgacagct tagatttcga tcatcatcct
    26941 aagcgcaggc caaaacgcca ctcatcaatc gacgcggaaa agacgatatg accgaccagc
    27001 agctgtttac cccttacacc cttggcgccc tgacgttagc gaaccgagtt gtgctcgcgc
    27061 cgctgacacg caaccgtgcg ggcataggtc tggtgccgag cgagttcgcg gcggcctatt
    27121 acagccagcg cgctagcgca ggcttgctga ttgccgaagc ctcgcagatc tcccagcagg
    27181 gacagggtta tcagaacacc ccagggatct acacccaggc gcagatcgat ggctggcgca
    27241 aggtgaccga tgccgtccac gcccaaggcg ggaagatttt tctgcagctg tggcatgtgg
    27301 gccgcgtttc ccacgtagac ctgcaagcaa acggcgccgc gccggttgga ccttccgcgc
    27361 tgcgtgccgc caccaaagtg ttcgtcaaca acagctttgc ggatgtctcc gagccgcgcg
    27421 cgctggagat tggcgagctg ccgggcatcg tcaatgattt ccgccaagcg gcggcaaacg
    27481 cgattgccgc aggctttgat ggcgtggaaa tccatggcgc gaacggctat ttgctcgatc
    27541 agttcatcaa ggacggcgcc aacgtgcgca ccgatgccta cggcggctca atcgaaaatc
    27601 gcgcgcgcct gttgctggag gtgacggctg cggtggttga cgagatcggc gctgatcgca
    27661 ccggtattcg cctgtcgccg gtgtcgcccg ccaacggggt ttcgagcagt gatccgcagg
    27721 cgcagttcga ctatatcgtc gatcaactca acgccctgga catcgtatac ctgcacgtgg
    27781 tcgaaggcgc gaccggcggg ccgcgcgacg tggcgccctt cgatttcgcc gccatgcgcc
    27841 agcgcttcaa gaacacctac atcgccaaca acggctatga cctggacctg gccacctcgc
    27901 aactcgctga agacaaggcc gacctgatcg ccttcggtcg cccctttatc ggcaacccgg
    27961 atctggtgga gcgccttaag gccggcgcgc cgttgtccgc gttcaaccca gccaccctct
    28021 atggcggcgg tgcggtgggc tataccgact acccaacgat gactgaatcg aacactgccg
    28081 cgaacccggc ataagttgat cacgcctgat ggcttcaccc atcaatccct atcgatgtcc
    28141 tgaaaaagag ataaccgcat gagcactcgt cctaccgttc ttatcactgg cgcttccacc
    28201 ggcattggcg ctgtttacgc cgagcgtttt gcccagcgtg ggcatgatct ggtcctggtc
    28261 gctcgcgacc aggcgcgcct gcacacgctg gcaacccgct tacacagcga acacggcgtc
    28321 gctgtagatg tgctgcaagc agacctgacc caactcagcg acctgacggc gatcgaagcg
    28381 cgcctgcgtg atgacgccag catcggcatc ctggtcaaca acgccggtgc cgcgcagtcc
    28441 ggcagcttta tcgagcagag cacagacagc gtcgcgcatc tggtcgccct gaacaccact
    28501 gcattggtgc gcctagccag tgccatcgct ccacgcctgg ccaaagccgg taatggcgcg
    28561 atcatcaaca tcggctcggt ggtcggtctg gcgccggagt tcggcatgtc ggtctatggg
    28621 gcgaccaagg catttgtgtt gttcctgtcc caaggcctga gcctggagct ttcgcctcaa
    28681 ggtgtttatg tgcaggccgt gctgcctgcc gccacccgca ctgagatctg ggatcgcgcc
    28741 ggcatcgaca tcaacacctt gagcgacatc atggaggtgg gtgatctggt cgatgccgcg
    28801 ctggtcggtt tcgaccgccg cgagccggtg accattccgc cactgcaaga aggcgagcgt
    28861 tgggacgttt tgcaaacagc gcgtcagggg ctgctgtcgc agatccgcca gtctgctgtc
    28921 gcacaacgct atctgaccca agcctgagtt tgcagaacac gtcggccccg gcccaggacg
    28981 cactaacaat gaccttaatg aatgcacaac atctgtttgg ttcgcgggcg tcattcgtcg
    29041 cggccgcgaa ccaatcgatc agggttgacg gcacagcctt cgcctaccgc gacctcggcc
    29101 ccaggactga ggtgccgctg gttgtgttca atcattgggg tgcggtgctg gacaacttcg
    29161 accccaggat catcgatggg ctggcacaaa cccggcgggt gatcgccatc aactaccgcg
    29221 gcattggtgg atcgggtgga gccgcgccac tgaccgtcgc tgagatggcg gacgatgcaa
    29281 tcggcctgat agctgcgctg gggtttgcca aggtcgatct gctcggtttc tcgctcggcg
    29341 gatttgtcgc gcaagacatc gccctgaagg cgccagggtt gctgcggaaa atgatcctca
    29401 ccgggaccgg tcctgccggt ggcgcaggca tcgacagagt cggtgcgatg acctggcctt
    29461 tgatgctcaa aggcctgctg acgctgcgag accccaagtt ctacctgttc ttcaccgcgt
    29521 cggccaccag ccgcagcgct gcccgcgcgt ttttgcagcg actcaaggag cgcaagcttg
    29581 atcgggacaa accggccacg cctcgggcca tgctgcgcca gctcaaggca atcaaggcct
    29641 ggggccggca ggcgccgcaa gacctcggca gcctgcgact ccctgttcta atcgccaatg
    29701 gcgatcagga tgccatggtg cctactgctc tcagtcggga tatggcacac cgtatacccg
    29761 gcgctcaact gatcatttac gacgacgcgg gtcacggtgg catcttccag taccacgccg
    29821 atttcatctc cgatgcactg gcttttctag ccgagtgact gcgtccttta ccccgaaatc
    29881 cattgctcac ggagcaccga accatgcacg cgttcatcat tgatcgttac ggtaagaaag
    29941 cagttggccg catcggcgaa gtgccggctc ccgacgtgcg gggcgatgac gtgctgattc
    30001 aaattcacgc ggcgagcgtc aaccctctgg atagcaagat caagagcggc gaattcaaac
    30061 tgattctgcc ctaccggctc ccgcttattc tgggcaacga cctggccggc acggttgtgc
    30121 gggttggcgc tcgcgtgcgc ggcttcaagc caggcgacga ggtctacgcg cgccctgacg
    30181 atgaccgtat tggcaccttc gccgagttta ttgccgtcaa ggcatcgtca gtggcgttga
    30241 agcccaagaa cctcagcatg gtcgaggccg ccgctgttcc gctggtggcg ttaaccgcct
    30301 ggcaggtgct ggtcgaaacc gccaagctga agaaggggca aaaagtcttt attcaagccg
    30361 gctctggcgg cgtgggcagc gtcgccatcc agttagccaa gcatctgggc gccttcgtgg
    30421 ccacgaccac cagtacggcc aatatcgact gggcgaaggc cttgggcgcg gacgtggtca
    30481 tcgattacaa aaagcaggac ttcgccagcg tgctgcgtga ctacgacgtg gtgctgaaca
    30541 gcctcggcaa cgacgagctg aagaaatccc tgcaaattct caagccgggc gggcacctta
    30601 tctccatctc cgggtcgccc accccgacgt tcgccgaggc gcgtgggttg gcctggccgc
    30661 tcaaacaggt gctacgtctg ctgagtttcg gcattcggca gaaggccaag cagtcgaatg
    30721 tcgaatacac cttcgtgttt atgcgcgccg atggggtgca gttacgcgag atcacttcgc
    30781 ttatcgaatc aggcgcgatc cgcccagtcg tcgacaaaat atttccgttt gcggacaccc
    30841 acaaggcgtt ggcctacgta gacagtgggc gcgccaaggg taaggtcgtg gtgcaagtga
    30901 tttaactggc gcgcctgttt gcgcaagcct ctttgatcag gttcatgaac tgtagatttc
    30961 agtagtaggc tgcgtatagg agctacgcga gtagcgcttt atgcaactct aactaagtca
    31021 gtttcgaagc tgggcggtga gtgtggacag gtttcaggag atgaaggtac tgctggccgt
    31081 tgctgaggcg caaagctttg ccggtggcgc caagctggtg ggcatgtcgc cacccagcgt
    31141 gacccgggtg attgccgggc tggagaagcg tttgggtaca ctgttgctgg cgcgcagcac
    31201 gcgtagcctg cgcctgaccg aggcggggcg gcgttatgtg gaggactgca aacgcatcct
    31261 gctggagctg gaagaggccg aagagcttgc caccggcagc agcattcggg cacggggcaa
    31321 cctgaccgtg acggcgccag tgatgttcgg cgagctgtac ctgatcccgt tgattaccga
    31381 gtacctgtcg gcgcatccac aggtgacgat caatgcgcta ttggtggatc gcctggtgaa
    31441 catggtggag gagggcgtgg acgtcgccat ccgcatcggt cagttgccgg aaaccggcct
    31501 gcaggccatc aaggtcgggc acatccgccc ggttatctgc gccgcgccgg cctttcttga
    31561 gcgctttggt cggccgcagg aacccaatga ggtgatggcc gcgcccatcg tgatgtcctc
    31621 tgccagcagc ctgctcaccg actggcagtt cagcctgggc accagctccc tcactctgca
    31681 tcccaaaccg cgcctgatgg tgagttccaa tcaggcggcg atcaatgctg cgtgcctggg
    31741 ttgggggctg acgcgggtgc tgtcgtatca ggtggccgag cgggtggcga agggggagtt
    31801 ggagatcatc ctcgagacct ttgaagccgc agcaataccg gtgcatgtgc tctatcaagg
    31861 cagcggtcgg gtaccggcca aggtgcgcac ctttgtcgac ttttgtgcca gccgcttcct
    31921 gtacgaccct gcgctgcaaa aggccgggag cgcctagcgc atgagccgct atcggcagat
    31981 gcaggtgttc gccgccgtgg cgcaagccgg cagtctggcc gcggccgcgc ggcagctgga
    32041 gctttccccc gcgaccatca tgcgcagcat tgccgcactg gaagcgcgcc tgaataacac
    32101 cctgctgctg cgtggaccgc gtggcgtcga actgagtccg gcgggtgagc agtttgctgt
    32161 gagttgccgg caccttcttg agcaagccac gattgctgaa cgctccgctg ccggtatgca
    32221 tgcgagccct gctgggcggt tgaccctgtc gttaccgctg ctgatggatc agcaggtatt
    32281 gatgccaatc gccctcgact atttggcggc attccccgac gtgcagctgg tcacccgggc
    32341 cagcgaaggc gtgccgaaac tgcttgaaga gggcattgat atcgccctgg tggtgggcca
    32401 tttgccgaac tcttccggct ttgccatgcc ggtgggcagg gttcagccaa ttgtctgcgc
    32461 atctcctgta tacctggcca agtgggggcg cccgcagata cccgatgacc tcaaaacgca
    32521 ccgtgcggtg ttgaccacgg cagacggctc gcaaaacgaa tggcgctttc gcgatgagcg
    32581 caccacacgc ctgatgaagc ccgcgcccgt gctgacctgc accacccagc gcggcgccat
    32641 ccatgctgcg actctggggc tgggcctgat tcgctgcatg agttttgagg cgcaccatga
    32701 attgcagagc ggcttgcttg agcccgtgtt gagcgacttt gccagccagg acctgcccgc
    32761 gcagttgatc taccgcgatg gtcgccgggc cgaggcacgg gtgcgtacct ttatcgactt
    32821 cgccacgccc aggttgcgcg ctcatccggc atttcttggc taacagccgc attcttttac
    32881 aaaacgaaat tatgcattgc cctgcatggt gattctgctt ggccgtgagt gggtgcagca
    32941 tggacctacc agcgcaagag ccgccgattc ggtttgacgc cgctgattac cctgacctca
    33001 cacggagccg caccatgtct cagccagcca tcaaacttta tcgtgtccct ctttcaggcc
    33061 atgcccatcg cgtcgaactg atgctctcac tgctgcagct gcccaccgaa ctggtgtttg
    33121 tcgacatggc caacggggcg cacaaaaaac ctgagttcct cgccatcaac gctttcgggc
    33181 aagtgccggt tatcgatgac aacggcacca tcctcagtga ctccaacgcc atcctggttt
    33241 atctggcgaa gaaatatggc gacggtaagt ggctggctga agagcctctt gctgccgcgc
    33301 gcattcagcg ctggctgtcg gtggcggccg gccaactggc ttacggcccg gcatctgctc
    33361 ggctgatcac ggtgttcggt gcgccattga atgccgaaga agtgatcgcc cgttcccacg
    33421 ccctgctcaa ggtgatggac ggcgaactgg ccagcacgcc tttcctggcg ggcgacagcg
    33481 ccaccctcgc cgatatcgcc aactacagct atatcgccca tgcaccggaa ggcaatgtct
    33541 cgctgcaggc ctacccgaat gtgcgcgcct ggctggagcg ggtcgaagct ctgccaggtt
    33601 ttgttcccat gcagcgcact gcagcaggcc tgcaagccgc ctgagtcgcc acaggctgcc
    33661 gggcttcccg gcagccttct tgaggagcgc attgcatgga tcatctgcct aaacaccgcc
    33721 gctcaccctg gcatgccggg gaaaaaacgc ttcaggaaac ctacggcgtc tccgaacgca
    33781 tggaggtgat cgggcagaag gttatccgcg attacatgcc cgaccagcac cgcgagttct
    33841 accagcaact gccctttatg gtcattggtg cggtcgatgc acagcagcgg ccctgggcga
    33901 ctttgcttga ggggccggaa ggtttcgtga cctcgcccga cccgcagcag ttgctagtgg
    33961 ccgtacaacc cgacgcgcag gacccagccg ctgccggcct gcaacccggt caggccattg
    34021 gcttgcttgg catcgagttg catacgcgtc ggcgcaatcg catcaacggg gtgatccagc
    34081 aggtgtctgc cgctggcctg gccgtggcgg tcgagcactc ctatggcaac tgccccaaat
    34141 atatccaggc gcgcggttat agccgcacgc cggagccgct gaagagccgt gcatcgcgcg
    34201 aggatttcac cgcgctaaat gcccgcacca ccgagatgat ccgcgcggcg gataccttct
    34261 ttatcgccag ctacttcgac catgacgccc acagccgctc ggtggacgta tcgcatcgcg
    34321 gcggccgcgc cggctttgtc aaagtcgaag gcaaccggct gaccatcccc gattacgccg
    34381 gcaatctgtt cttcaacact ttgggcaacc tacaggccaa tcctgtcgcc ggcttgctgt
    34441 ttgtcgattt cgccacgggc gacatgctgc aactcaccgg ccgcaccgag ctgattctcg
    34501 atagcccaat gatcaaagcg tttgaaaacg ccgagcgcct ctggactttc gatgtcgagc
    34561 aggtggtgct gcgcccggct gccgtatcgc tgcgctggga atttcacgac tacgccccca
    34621 ccagcctggc caccggcacc tgggcagagg cggacgagaa gctgcggcag aacgagcaac
    34681 gccggcagtg gcaacaatgg cgtgtgactg aggtgcggca ggagagtcag gacattcgct
    34741 cgttttatat ccagccgctg gataaagcag cggtggcgtt tacccctggg cagcatatgc
    34801 cgctacgcct gcaaacactt ggtgctgaag cgccgttgat ccgcacctac agcgtctcca
    34861 gcgcgccgtc tgacgggcat attcgcatca gcgtcaaagc ccaggggcca gcctcgtatc
    34921 atctgcataa gcaggtgcag gtgggcgatg tgttggaggt tcgtccaccc atgggcagct
    34981 tcaccctgat cgaagatacc gagcgcccgg tggtgttgat cgctgccggg gtgggcatta
    35041 cacctttgct ctcgatgttc cgcgagctgg ctgcgcagaa tattcagcag cggcaacgcc
    35101 ctgtgcatct gtttcaaagc gcccgaaccc tggaagacct gccgtttcag gccgaactca
    35161 aggcgctgca gcaacgcgat accgagcacc tgcatatcca tcgggcatta agccgtccgg
    35221 gcactgaggc ggttgtgggg cgcgactttg agataaccgg tcgtctcgat tttgcccagg
    35281 tcaaggcgcg actgccgctg gatgactacg acttttacct gtgcggcccc ggcggttttc
    35341 tgcaggctat gtacgacgga ttgcgtgggc tgaacattgt cgatgaacgc atccacgctg
    35401 aagccttcgg cccctcgaca ttgcagcgct tagcggttga agcgcttgct gcaccggcac
    35461 agttactcgc cgccagcgcc ccggtgccgg tctatttctc ctcgtcggcg aaagaggcgc
    35521 gctggacacc gggcagtggc agcttgctgg agctggcgga aagccgcggt ctgagccccg
    35581 acttcagctg ccgtggcggc tcgtgcggca cctgcaaaac gcgcctgatc agcggcaacg
    35641 tgcattaccc caacccgccc gcgcaactgc cggagggtga cagcgtattg atctgctgcg
    35701 ccgtaccggc ggagctggac ggtgctatcc aacccctggt actcgatctc tagtgaggtg
    35761 ctgcgacatg aaccgcgaaa tagttgaccg ttacgacgtg ctcgacgcct cgagtgaaac
    35821 gccagggcac tgggaagtga attatttact gccgcagttg ctgtctggtc ctgagtaccc
    35881 aggccgcgac gtaaatgctg cgctgatgcg tgtgttgaag cggctgtttg ccgacttacg
    35941 ggcaccccgc gtcgtgccac ccgttgcgcc atccctcacc ccatacagga gccaccgcca
    36001 tgtctaccgc tgaaatccgc caacctattc caccctttac ccgcgagtcg gcgctgttaa
    36061 aagtccgcct cgccgaagac ggctggaaca gccgcgatgc ggtcaaagtg gccttggctt
    36121 ataccccgga tacccaatgg cgcaaccgcg ccgaattcgt cgctagccgt gccgaagctc
    36181 aagcgttttt agaacgtaaa tggtgcaagg agctggagta ccggttgatc aaggagttgt
    36241 gggcgtttac cgataaccgc atcgccgtgc gttatgccta cgaatggcac gacgactcgg
    36301 gcaactggtt tcgctcctac ggcaacgaga actgggagtt cgctgagaac ggcctgatgg
    36361 cccaccgctt tgcctgtatc aatgatctgc caatccagga gtccgagcgc aaattccgct
    36421 ggccactggg ccgtcgtcct gacgaccatc cggggctttc gcagttgggg ttgtaggttc
    36481 agtcgacttc ggattgttag tgttgcgcgg aagctggagg tagcccacct gccgatcagt
    36541 ggcgtctatc aatctagtga cggttcagag gaacaataca ttcattccac tgttggattg
    36601 gcgggctcta gctccaagcg gacataacta gcctcgcgtc gaagcccgtc cgctgtatag
    36661 gtcttcacca gttgaaggtt ctcagcgtca taagtgatgc cgtcgacaag gcaggcttca
    36721 gcgactcggg gggtgttaat ttgcagccag gcgtaatgtt tcttcgtcca ttcaagaatc
    36781 gccttgtgca agggtcgatt ttccttgtcg taatcaccct tgcgcacttc caagcccata
    36841 acattcagac agaacccgag aatttttgcc cctttaaaat tagggaagcg attcatttcg
    36901 atgatctcgt catacaaaag ccggcgcagc ttaaatttta ttactagacc cccaggccca
    36961 ttgagctcgc gggtaatcgg cccccaaatc gagttgtgct ggatagacca gcattcccac
    37021 tgtggcgaaa ttacagacga cgcgtgaaag atgacctcga aaatcatgct cgctatgtca
    37081 tcgtagagcg actcacgtgg gtggccatac tccactctca cgcgaaggcg gacttggttg
    37141 ggctttccct ttttatcgag aattttgacc gcgtcggtaa tgaaacccat gactacctgg
    37201 agacacttgg cggcatcgct ttcccacgta ttggctaccg cattcagctt atacaagccg
    37261 gaaacagcgt tttcgatgta gtcctttgcg cggtaaaggg cggtagagtg attccagtgc
    37321 ggtttatcca catagtcgcg gagagtaatc aaaaccagtc tgcagtaggc ctcccactgt
    37381 acggcgttcc atttcaattt gccgacggta tcaggatcaa gcagtgtcct aatggtgtcg
    37441 accattttgt ggtttccgaa catcgcttgg cttaggggtt tgtggtatcc aatcaatcct
    37501 gattcatatc ccattgcttc gtgatagaga aacgagttcg tgttcagcag agcttctctc
    37561 acgatatttt tagcgaaaat ttcgacttgg atgtcgtatt ttttcgtctc gcccatctcc
    37621 tcaaagatcg ccaatgcggt acccgatgaa gattctacga ttacacggca gaggcgctta
    37681 tcggctatta gaagcaggat atcgttcgca taggcttcca ctttcggtaa gtccggatgc
    37741 aattcgtctt catcctgtaa gcgacgatga tttcgaagca tccttcggtc ggtcgcgtag
    37801 tgcactagtg ctctggccga gtacgtgagc tcgtcagcga ccacggccaa ctctgtcgga
    37861 tcacctttaa gaatgtgtcg atacaaagcc tgcgcatagc gttcggcgtt ccgtttccca
    37921 aatgttggtc gtctaataaa ggcgaaccat gtccaggtta ggaatgtgag gaggaatatc
    37981 cctgcgagga aggcctgcca tagaggtggt gtgagaaaac taccttgagg cacgagccat
    38041 ccttgagcgc gccataggtc ggtcaacaag gtgagtacac ccacgatagc aacgacggag
    38101 aaagttacgc gttggagcgg aatcggggcg gtttgcacgc gaaagtggta gcgcacgtcg
    38161 ccgattgtcc aggcgagaac catgagagca agaccggcga gaaattccga gaagccgaaa
    38221 aatttagatc cattcggatc aagcgaaatg aagcaaacag ctgcaaagca cttctccatt
    38281 accatgatta tccttttaga gtttatttct cgggcatgta ctcaggggct tccctgtgag
    38341 caaaaaattc tcttgtagga taatttttaa ggtcgagagt gagagcaact gtctcacgaa
    38401 tcgaagtaat ccacttcttt ggttctaaac ctttaaacag cgtgcctgcc gataacacac
    38461 cggctagcgt ttcactcagt ttgtgtggcc taagccatct cggttcgccc agcgagtcgc
    38521 tgctctaagt ccacacaata aagccgctca tatgagcaac tttattgtgt gcggcgttaa
    38581 ccgcgcgata gcggcggcgt acgcggcggc acccgtcgct tcgagccggt ggactcgaac
    38641 gcggcagcaa agcgcagcag ggcggagtcg tcgtaggcgc ggccggcgaa ggtcaggccg
    38701 gctggcatgc cgatatcggc catcacaccc atcggcacgg tgacggtggg cacgcccagg
    38761 tgacggatgg cgaggttgcc gttggccacc cagacgccat tgctccaggc gatatccgca
    38821 gactctggat tgacgtccgc atccgccggg ccgacatcgg cgacggtggg gaacagcacg
    38881 gcgtcgaggc cgaggttatc catccagtct tccaggtcga gcttgcgggt gtgttccagg
    38941 ccgcgtaggc catcggacag ggtttcgatc tgatcccagt tcttcaggcc gcgtttggcc
    39001 atgttgacgt attcatccat gccagcggcc aggtcgcctt cgcggttggg cagggtgccg
    39061 gggtcgtggg ggaaaatctg cgggccgtcg acgtcggcca gtttgttcag cttgggatcg
    39121 ccgttggcgc gcagaaagtc gtcaaaggcc cagccgctca gttcccacaa ttcgtcgtgg
    39181 aggaactccg gagtgacgat gccacggttg taaacggtcg gcgcgccggg acggtcgcct
    39241 tcgcagttgg acaccagcgg aaagtccact tcgatcactt cggcgccagc agcttccagc
    39301 gccttgcgcg cggcttccca caggtcgatc accgaggcgc gggtgtggat gcgctggccg
    39361 gttgggccgc cgatgccggg gctctcgctg gtgccggcgg cgtcatcctt gttgataaac
    39421 atgcgcggca cgccaaagcg tttgccggcc agggcatcgg ctttggcggc gagatcggca
    39481 taggcggctg ggcgcacctc ggaggctttg gggatcggca cccagggctg caggcgccac
    39541 agatcgccac ggttgtccgg gtcgtcggcg accacgatat cgagtatttc gagcaagtcg
    39601 gccatggttc gtgcataggg caccaccacg tccatggtcg gtgtcagcgg ccagttgccg
    39661 cgcaccgaaa tcaccccgcg cgatggcgtg taggcacaca ggccgttgtt cgaggctggg
    39721 ccgcgaccgc tcgaccaggt ttcctcggcc agaccaaaag cggcaaagct ggccgcagtg
    39781 gccgtgccgg caccgttgga tgagcccgag gcgaagggcg cggtgaggta atcggcgttg
    39841 tacgggcttt ccgcccggcc atagacgccg cgctgcatgc cgccgttggc catgggcggc
    39901 atattggttt tgcccaggca gatggccccg gcgccgcgca gacgctcgac ggtaaaggca
    39961 tcgcgatagg cgaccaggtc tttgaacgct gggctgccgg aggcggcggt gaggcccttg
    40021 accaggtagc tgtccttggc ggtgtagggg atgccgtcga gcgggctgag ggctgcgcca
    40081 cgggtgcggc gctcatcgga tgcttgggct tccttgagtg catcggggtt gcgtaccacc
    40141 acggcgttga gctgtgtcgg cgtgccggca gcgtcatagg catcgatgcg ggcgagatag
    40201 gcctgcacca gttcgaccgc cgtggtctgg ccggcttcga gcgcggcgcg cagtgcggca
    40261 atgggaagct cggttacctc gatcatgctg tcaccgctgg ctgttgaaga gagttgttca
    40321 tatcagttct cgtggcgctg ttcttatgcg ttgaataggt tgcaggcgct tattttagcc
    40381 acgcgccggg gtgaccaggc ccttgattac tgcggggcgg gcgacaaagg tttgcaacac
    40441 tcgctgaaca ttggcgaagc gctcgaactc caccagctcg ccagcctcgt agaagccaac
    40501 caggtttcta acccagggga atacggcgat atcggcgatg ctgtagtcct cgcccatgat
    40561 ccaactgcgg ccttgcaggc gctggtcgag tacgccgagc aggcgctggg attcggcgac
    40621 gtagcggtcg cgcgggcgtt tgtccgggta atccttgccg gcgaatttat ggaagaaacc
    40681 cagctggccg aacatcgggc cgatgccgcc catctgccac atcagccact gcagtgtttc
    40741 gtagcgcgtg gttgggtcct gggccaatag ctggccggtt ttctctgcca ggtagatcag
    40801 aatcgccccg gactcgaaca gcgccagcgg tttgccgcct gggccgttgg ggtcgaggat
    40861 cgccgggatc ttgttgttgg ggttgagcga gagaaattct tcgctgagct gatcgttagt
    40921 gtcgaagctg accaggtgcg cttcgtaggg caggccgatc tcttcgagca tgatcgaggc
    40981 ctttacgccg ttgggcgtgg gcagcgaata cagctgcagg cgctcggggt gctgggccgg
    41041 ccatttgtgg gtaattgggt aagcggcaag ggctgacatg ggcgctccgg attaaggtga
    41101 cgaaaggcgc aggatagcgg tgattggcca tttggtcagg ttgcaggttt ttccccgcgc
    41161 gcagaagtcg gtatggttgg ggccatttca acccccgcgt attggagaaa cctgatgagc
    41221 ctgcacggcg aatacgacaa ccagaatatc tttgcccaga tcattcgcgg cgaagcccct
    41281 tgctataagt tgtacgagga tgaggatgtg ctggctttcc tcgatgtgtt tccgcagtcc
    41341 tttggccata ccctggtgat ccccaagcgt gcggcggcgc gcaatattct ggaaatcgat
    41401 agcgacagcc tgaacaaggt gatggctgtg gtgcagaagc tgactggggt gatcatcgcc
    41461 gaactggaac ctgccggggt gcaggtggcg cagttcaatg gcgcgccggc cgggcagacg
    41521 gtgttccaca ttcatatgca tatcgtgccg cgctataccg gcgagacgct cagcgtgcat
    41581 gccggcggca aggcgactcc ggaagagctg gcggcattgc aggcgcgtct gatcaagcgt
    41641 atcgcgggtt gatataaccc tggttgcggc agcgtacttg agagcggccg cgtcaggggc
    41701 aatactcaag ccgttttgcg gttttccctt tgctgttttc tgcgcccatg gagggctccc
    41761 atgcgattga tttgtgccgt tttaccgctg ttgctgttgg ccggctgttc gtcctggaag
    41821 cccgcgccag aggatgtcaa acccgtgcca gcggaccgct tgctgggcta tcaggcgccg
    41881 ctggtgcagg gtggagagtt gcaggtcaat cgcgacttcg gcggcatggg cggcggttgc
    41941 tatgtggcgg ttctggttga tcgcaaggtg gctgcgcgca tcggcgtggg tgagcaggtg
    42001 cgtttccagg tgccggtcgg cacacgggtg ctgagcattg gtatcgacga gatggacgac
    42061 accctgtgcg gcatgggccg cctgcgccgc gagctggccg tgaaagtcga gccgggctca
    42121 cagcagaatt tccgcatcgt cagcgataac cgcaaaggtt tcgacatcct gccgattacc
    42181 cagtaacgcg caacgtaccg gtgcctgctt gtcaggcgcc ggtttccttt ctcagccagg
    42241 ttttgcggtt tgctgtgccg tggcgcacag cggtgcagcc gctgtggtga tgcctgcaag
    42301 tcgcacgggt agaatccgct cggtttaata acggagacgg gtcgtgaagg gattgccgat
    42361 tgctattgtc ggggcaggta cggcggggtt ggccacggcg ctgttcctca cccgtcaggg
    42421 tttcagcgtg cgcttgctgg agcgtgtagc ggagctgcag ccggtcggtg ccggggtgtt
    42481 gttgcagccc tcggggctgg cggtgttgca gcagctgggg ctgtttgccg agtgcagcgg
    42541 cctgggggct gcggtcagcc gcttgtacgg cacctcggct aatggccggg tgattctcga
    42601 tacccgttat gcgcactggc agcccggcag ttttggtatg ggcattcacc gcagcgtatt
    42661 gctcacggct ttgctcaacg cggcgcggca agccggtgtg cagattgaaa ccggtgtgca
    42721 tatcagccgc tttacccagc atggcagcca tgtgcagttg tatcgccagg acgagcaggg
    42781 cgtggagcag ccattcggcg aattcgccgc gctggtgctg gccgatggtg tgcgctcggt
    42841 tctgcgcggg cagatgcagg tcaagcagtg gctgcagcct tacccctggg gcgcgctgtg
    42901 gagcattgtg ccgaccccgg cttcgtcgcc aaccgcacag gatgccacgg acctgcgcca
    42961 gtggtaccgg gattgcgcgc agatgttcgg catcatgccc actgggcgta cccacctgaa
    43021 ccgcgaacag gcgctgagca gcctgttctg gagcctgccg gtggcgcagt tcggcgcctg
    43081 gcgcgaagcc ggcttggccg cctggaaagc ccaggtgctg cagctggccg gcagtgctgc
    43141 cgaacccttt gttgagctga tcgagcgtcc cgaacaactg agccaggcgg tgtatgccga
    43201 tgtacgcatg cagcagtggc acgacgggcg ggtgatcgcc atcggcgact gtgcccacgc
    43261 catgagcccg caactgggcc agggcgccaa tatggcgctg gtggatgcgg cagccttgtc
    43321 cgccgcagtc atcacacaca gcacgcaggc caactgcgac tggtcagcag tattcgccgc
    43381 ctatgccagc agccggcgtg accatctgcg ctactaccgc caggccagtc gcctactcac
    43441 gccactgttc caatcccaca gccgcagcct tgcctggcta cgcgatggcg tgctgctgct
    43501 ggcgcggcac aatccatttg gccgtgcgca tgcggtcagc accctggtgg gtgggcgcag
    43561 tggttggctg ctggccggtg ccgagcgacc gttgcagata tggaatgggc aggccaatga
    43621 cggcggctga ttttggtcga ttacagcctc ttgcaactgg cagcagtcgg ccagaagcgg
    43681 tcgctctgcc atcgatctca catggtctct aacgcggagt taagagctgc cagcttgggc
    43741 gctgtgttaa gtttggatgg ctagatgagg attattttta agtattgcag tcagtataat
    43801 ctctaatcta gtgccgataa aataaccgcc atttttcaga tatgtatgtc cgatgccaat
    43861 aagcttgcga tcatctggga tcaagtcttt aagtacgggt ggccatataa aaattcctcc
    43921 cccgcttatt ccgtgtggca gaggtgcata ttgtatcttg ccaattgatg ggtcgacggt
    43981 tttgtttctg ttgtattttg caactatgtt gagtctaggt ttgcacctaa gcgtggcata
    44041 gtccgaagcg gctgcgtgat acgctccata agcgtactct tttgtagttg ccacgctgcc
    44101 agctacatta tgctttcgga taggaaagcc gccgaatgtc attagctcct tgtcgctata
    44161 gtcgcccttt attccgaact cgctttcagg tatggcataa aataatttct tcattccttc
    44221 agcgaatgat ttttcaagct tgaaatatcc gaagtccaag ttatcggaat ttctgccgtc
    44281 acttggatcg atataagaat agcttccatc aatctcaaca attttattgt catagtgagg
    44341 caccattaaa gcgccgcgct caaatccgtc aataacatgc cctgcggtta atagaaaagc
    44401 ttcccctcta aactcaatca acaccccaga cccgaaaaga ctgaagcgct caccttctat
    44461 gtcaataaag atcggaacta atgcctcaag atgattgcgc atattgctag aatttctttc
    44521 tggttaagtg cggattgcgg ctactattca gcgggcgaag ctaaatactt taaccgctca
    44581 ggtacgtctt gtttaatgct cgcaatagtg tttcgcttgc tgaacgtgtt gcacataata
    44641 gtgtacaatt agcttttgcc gaccaagcga taaatatcaa ccacatcaaa cagtgttatc
    44701 aaataaagtc cagatttccg cttgcggtca gagcaaaaga agaagcctat taaatttttc
    44761 ccaccaggaa tgaaaggcac aaaatggtgg ctgtgaataa actgattgca gagatcgtat
    44821 attgatattt cggtgttttt cagcgccctg aagtcgtact gagcttcttt tgtatttgta
    44881 gctagcgatg tgggtttttc gcagtaaggg acttcatgga tattgtattt ctttcccgat
    44941 atatggtcgg gcactctctt tgactcaatt aattttctaa ctgaataaaa gccgatgaat
    45001 atttctttct ctactgtgta gaagtttttc tcgccccaac gttcttgaat cagcctgcgc
    45061 tcaagcctac atgcaagctt gagcaagtca tttttccaat agctagattc ctggatcata
    45121 agtatctact caagtagaag cgcgctggcg gggctgcggt atttagtaaa tgctctaagt
    45181 aaagaatggc tatatttact ctttactcta gcccccccgt tcaacctgga tatgcactcc
    45241 agcactggcc ggtcccggcc aagagcagac tgagtcgtac taagccgagc gaattcaata
    45301 gaggtgtaga cacactgtaa gcgcgcctac tcactccttc cgcaaacctc cagaatcaga
    45361 tttcgaagcc atcggtgagc cggatcagcg ctgaatcgtg ggtgccaaag caatgaaacg
    45421 gtgaactctg cagtacggaa aggaagcgga aaactataca taccctcgcg caagctcgcg
    45481 gtatggcgct cgggaacgct ggcgatcatg tctgaccccc gcgccaaccc cagcgcgatt
    45541 gaaaagccac caacgattgt ggctatcttt cgctccaatc cgagtggcag cagcgcgtca
    45601 tctatgggac ctttttctaa accttcccgc gacacggaga tatgcatgct cgcagcatac
    45661 ttttccggcg tgatagtgct gcaacttaat ggatgactgg ctcgtacaac gccaacgtaa
    45721 ttatccctga acaaagactg gcccttcagc tcgggggcag aggccagttc tataacaccg
    45781 gtttcaagat cgacgactcc atcgcgtaat ggcttactgt ctttgtctgg cttttgcata
    45841 aagaacagcc gtactccagg ggcctgctct ccaatacgcg cgataagtgc cggaccaaag
    45901 ttctcggcaa aaccctcact ggtcctgatc gcgaatgtcc gatcaagttt ttttaggtca
    45961 agaagctcta ctgggcgcaa aacctcctgc gcatcacgaa caagctgact gacgcgatca
    46021 cgaagttcaa tcgccctggg agtaggaacg agcccacgcc ccgccctaac caaaagcgga
    46081 tctcccgtgg tttctctgag ccgcgcaagt gcccggctca tcgccgatgg actgagccgc
    46141 aggcgtttag ctgcacgcgc gacacttccc tcagcgagca ctgcgtcgag ggtgatgagc
    46201 agattaaggt ctggattcga catagtccga tgttagccca actcgcctaa tacatggcgt
    46261 cagacgcaac aataaagtgc gaacagtgcg tcttccgcca tgtttatacg ctgcatacag
    46321 ttctctccat cggcacattc gtaccgaagt ggatgaggag atgatgtatg aactcgttga
    46381 gcagatttgt tgagggtgtc caatcaatct ggggcccgct cagttctgaa gtggttgcag
    46441 gctgccagca gcaactggat gaactcctga agacgcctgt caccgagccg tggctggctg
    46501 acctacgcgt gcaggcactg gctcaacagg agatgtaccg agatccggta aacggatttg
    46561 tgctgctggc acacaccgag cctgaagggc tctaccgacc gcctcatgat cacggtcgtg
    46621 gctgggtcat ttacgcggtg cagcacggtg aaatagagat gggcacctat gccaaggtgc
    46681 aggctccgga tggccaggtg aaactggtaa aacgcggttc cacacgggta agcgccggtc
    46741 aaacgcaggt ctatctaccg ggtgatattc acgatacgca ctgcgtttcc gggccggctt
    46801 tgctctttcg cttcaccgag cgggacttga agaaggagga caaagaggcc cgcaagctga
    46861 cacggtacat cgaacaggat ggtgattggg ttccggcgag ccagtgcgct cccgagcggt
    46921 tgaaatcatg aggctcggcc cagctaccaa gcagcatgct gttgacgcgc gatcagaccg
    46981 cacaaaaccg aggcacctgg cagccatcag ctcagtgctg gccgccatgg tgctggtggt
    47041 gctcaacacg gcgatagcca acatggcgtt gccaacgatc gcccagtcgc tgcaagtgac
    47101 accagcgctg tcaatctgga tcatcacggc caatcaggcg gcgctcctga tggctttgct
    47161 accctgcgcg gcgctgggtg aaagccttgg ctaccgtcgg gtattccggt ggggagtggt
    47221 gctttttctg ttcgccactc tgatgtgcgc gctggcgccg acgctgtctt ggcttatcgc
    47281 ggcgcggttg ctgcaggggc ttggaggcgc ggcagtcatg gcgctcgcag ttgcattgct
    47341 tcggcaagtg ctgccgagcc agcaactggg cgccgctatc ggctggaatg cacttgcggt
    47401 tgccctgtca tcagcagccg gccccgcatt gggggcagca attctcagcg tggcagactg
    47461 gccatggctg tttgctgtgc tgctcccatt cgggttctgg gtgatgctag cggcccgcgc
    47521 tctacctgct gtggacggca ccacgcgtaa ggctgatctg ttgagcattg cattgaatgc
    47581 cgggaccttt gcatcgctgg tcatagccgc tggagtcatc acggagcacc cagcactggc
    47641 tgcagcgttg ctggccatct ctgccatcca actgacgcta ttggttaagc gggaaaggca
    47701 gaaggagtcg ccactgattc cgcttgatct gctcagatcc aactcattcc gtatttcagt
    47761 tattgcctcg gtactctgct ttgctgggca gacagcaggc ctcgtggcgc tgccctttta
    47821 ccttcagcac agccttggac aagaggtgct gatgacaggc ctctacatga tcccctggcc
    47881 tttaaccgtc gcgcttgtag ctccgctagc tggccgtctt tcaaacagat ggtcgcctgc
    47941 ctggttttgt tcggtaggtg gcatttgtct ttcgacagga ctagctggag ctgcgctctg
    48001 gcctctgcag gaaaacctca tgatgcttgt gccactgacc atgctctgcg gtctcggctt
    48061 cggcctattt aacgtggcca ataaccgcaa catgttcctc acagcgccga tgagtaggag
    48121 tggtgctgcc ggggggatgc agggtacagc tcgcttgctc gggcaaactg ctggcgctgt
    48181 catcatgacg ctgctcttta acctgtttcc cgcccactca gcgcctcaac ttgcccttgg
    48241 cgcgggagct ttattgaact tgctcgcagc cctgataagc acactgcaaa gcagagagac
    48301 atacaactaa aaatcttgaa tttgaatcgc acctgacgct cttgacgaat attactagca
    48361 gcttctggcc gactctggcc tatcgcgaca ggctgaaatc ggccacaagc agtcattccg
    48421 gaagaaaaac aaatctgttc cctctcccct ttttagccct tttcaccgtt gcgcttgacc
    48481 ctattccctc ctcccggcca catgcgcgta ctgatcaaaa aattaacaaa ggaagaaccc
    48541 ctcatgcccc gcctggtttg gctacgcgac catatgcaac acagcgacac cctggcgcaa
    48601 tggctgcacc agcagttcgc atacgagttt atcgagcagc cattggctga ctggcagcgc
    48661 gaattcgcgg aaggccaggg caacggcgac tggcaatgcc tgatcgccct ggagaatgac
    48721 cagctacttg gcggtgctgc actcgccgcc agcgacttgt ccgaacgccc ggagctgggt
    48781 ccctggctcg cctgcgtctt tatcgccccg caggcaagga agcgaggcct ggcggagcag
    48841 ttgatagaag gcatctgcgc cgcagcaaag gccaacggca ccacacggct gtacctgcac
    48901 acccaggatc gcaacgacta ctacgccaaa cgtggatggc aggtacagga gtgttttgag
    48961 gcctggggca aggagcagtg gttgatgctg cgggatcttt gaactcgcct aggtttgaag
    49021 ccgaaggcgg agagcaaaca tctgggaacg cggatgctcg caacaggggg aaccggccag
    49081 aaacttatga tgcaggcgca tcaatatcgt ggtctgtccc caattgttcg cacgtgagac
    49141 ggtcgacacc atgcgcagtg gtgtcgagga agtgaatcaa ggcgtggcca tggctggctc
    49201 tgccggccag gcgatcgtgg gcattcgtga tagcgccaat aaagtgctgt acgtagtcga
    49261 tcagatttcc tttgccttgc gagagcagac agcagccagc caggacgttg ctcgaagcgg
    49321 ggagcggtcg gcacagatgg ccgagcagaa caaccagtct gtgcaggagt tgcttaaaac
    49381 cagcgcaagc ctgagcagcc atgcggcagc gttgcagcgg gaagtgtccg ccttccatat
    49441 ctgagattcg agacgctttg gcactccaca tgctgtgttg tcctaacgcc tgcaccggcc
    49501 tatcaaggtt cagttggagc aatgactgga tgtcattacg caagtggctc atgatatttt
    49561 ccagttaggc tcacgcctct tctgccattt ggggctctac gcatcgtgtc gagcattgcc
    49621 atctccgatc tgacggtatc tccaaacgtc gacagcgtct cgcggcccgt tttggccctg
    49681 agcgccactt tgctcgccaa agactgggaa aactccaggc atcagcaccg caaggcgcag
    49741 ctgatctact ccgttcgcgg catcctcaac tgcgaaatcg aagacggcgt ctggatcgtg
    49801 ccgccgcagt gcgctttgtg gatcccggga gacctgcccc actcggcatg gggctcgggt
    49861 gaagtggagt gctactgcct attcgtcgag ccggataccg cgctggatct gccgaaaacc
    49921 tgctgcacaa tttcggtatc acccctgctg cgtgagttgc tgttcaaggc gaccgctgtt
    49981 cctgagctgt acgcgctagg tggacgggag gaacggctgc ttgccgtgtt gctcgatgag
    50041 ctggtggcgg cgccagtgga agatttgcac ctgccgatgc cccgcgacgc gcgtttgcgt
    50101 cgcctcgcag aaatgatcct ggctgcccct gctgacaaaa cttcgaagac cacctgggct
    50161 acgcgcattg gcatgagcga gcgaagtatg agccgtctgc tgctacatga aattggcatg
    50221 agcttcgggc gctggcgtcg gcagctgcat gtgatcctgg cgcttcaacg gctgaccaag
    50281 ggtgaaagtg tgcaaacggt ggcgttggaa ctgggttacg aaaacgctag tggattcgtc
    50341 accatgttcc gcaaggcggt gggcaaaccg ccggcccgat acctctcgga ccgcaccagt
    50401 ggcgctgtga ctactccggt tcccgggata atgctccgcg accaagtcgc cctctgatcg
    50461 aaaccggctg ttctggtgta agcccactct gcttcatcga aaaaattgca ggcatgtgag
    50521 caccttgtgg cgacgacttc tcttcagcaa agcccccgcg aacccggcaa ctccacctct
    50581 ccctcgcgcc agaagatctc tcactcaaac agccaagcgc cgcgacgaca tcgccgcggt
    50641 gagctcgacc atattggccg aatcgaataa caatctggcc tgatttcgcc atcatgaccg
    50701 attgcctctg ttgttaaatg catcattcaa atacaaatga gtattgttaa tgagcgattt
    50761 agcaactgta ggtaagtccc actggcctat cgatatacgt gcagaagccg actcctatcc
    50821 tctgctggag tggacgtaca aagacggcat cagaggtgcg ccccttagcc tgctgggctc
    50881 ggctgtggcg gcagcaattc tcattggtgc gcagggtgca ttcgctgccg aacctgcgcc
    50941 gactcataac aaggcagtag gaacgtctca gggggagatc gtcttaccag caatgagtgt
    51001 gacaggccaa atgaacacgg cgatcaccga gggaaccggc tcctatacca ccggcgagac
    51061 ggcagcggcg acacgtctgc cgatgacgct gaaggaaact ccccaggcag tgaccgtgat
    51121 cacgcgccag cgcatggacg accagcagct caatacggtg aaaagcgtgc tcgagaacac
    51181 cacgggcgtc accgcgcgcc aagccgacag cgagcgcacc agcttttatt ctcggggttt
    51241 gctgatcaac aacgtgcagt acgacggtat ccccaccgtg gtcggcaaca ccgtcaacgg
    51301 cagcggtatt agcgcgctgg ataccgcatt ctatgaccgg gttgaggtcg tgcgcggcgg
    51361 ctctgccttg ctgaccggca tcggtaatcc ctcggccgcc atcaacctgg tgcgcaaacg
    51421 gccgacgcct gaattcgcgg cgtccgcctc gctcggggcc ggaagctggg atactcaccg
    51481 ggtcatgggc gacgtatcga cgccgttgac cgaggacggg cgtatccgcg cgcgtctggt
    51541 gggcacctat caggacggaa actcctatct ggatggctac aagcccgagc gtaaggcgtt
    51601 ctacggaatc atagaggcgg atctgaccac cgataccacc gtgagcctgg gctatgatta
    51661 ccaggacatc acgccgaagg gcgcgacctg gggcggcttg ccgctgtggt tcagcgacgg
    51721 gacgcaggcc gagtactctc gttccaagag ctacgcgcag gactggagtt attcggataa
    51781 caccctgaag acggcgtttt ccgaggtcga acaccgcttc gacaacggct ggaaactccg
    51841 tgctgtggcc aaccagtacc gatccgaaag cgaagcagaa ttgggctccc tgagcggtag
    51901 cccggatcgc gccacgggac tgggcacctt tcccagcggc gcctttccgg tggcactggc
    51961 ggccgagggg cgcagccgtc aaaacacctt tgacgtgatg gccagcggcc cctttgaact
    52021 gctaggccgt cagcacgacc tggtggtagg tgcgacaaat tcacgccgca aggccggcca
    52081 ggacgatgtg gcgcctttct tcgcaggctt tacgccagtc aatatctata ccttaagccc
    52141 aggctttgcc cgccccgact tcgatgccct gcctcacgtc ccgacccgca cccggatcaa
    52201 gcagagtggc gtctacagtg ctgctcgctt ctcgctgact gaaccgctga agttcatcat
    52261 tggcgggcgc ttcaataatt acgaagtcga tgacaacgtc aatggcactt cgctgcacta
    52321 taagaagaac aacgaattca ctccctatgc tggcctggtc tacgacattg acacgaccta
    52381 ctcggcctac gtcagctaca cgggcatctt caacccgcaa accgagtacc gggacagagc
    52441 tggcaacgtg ctcacgccgt ccaagggcaa aaccaaggaa gttggcctca aggccgctta
    52501 tctggacggg cgcctgaatg ctgcggtggc ggtgttcgag accgagctgg acaacgccgc
    52561 gcagcagatc cctggtgcgt tcaccccaag cggcgcccgc gcctataccg gcaccgatgg
    52621 caccaagtcg cgcggtatcg agttcgacct gcagggcgag ctggccagtg gctggaatat
    52681 ctacgccggt atcgcccact tcacagctga agatggagat ggcgtgcgcc ttaattccca
    52741 gttaccgcgc accacagccc agttcttcac cacctaccgg ctgcccggtg cgtggaacaa
    52801 gctgaccctg ggctccggcg tgaagtggca gagccgcttc taccaggcgc ctggcgccgg
    52861 caccagcacg ctgggcggcg aacagagcgc ctatgccctg acctcggtga tggggcgtta
    52921 cgccctcagc aagaacatcg acctgggcct caacgtgaac aacctgttcg acaagaagta
    52981 cgccctgcag aaaggtgact tcgacaccgt gacttatggc gccccgcgca acctgatggc
    53041 cacgctggac taccggtact aattgcccgc agggcggcga atcgccgccg gcgattcgcg
    53101 tcccaccacc ttgaacatcg agaacatgct catgcctacc ccaaacccgg ctcaagtatt
    53161 ggcgatacac aacgcaaata aggtcacctt attctggttg acgactactg ttggcgcggt
    53221 gctcttcgtg accttccagc tgtttttcta cgtaaataac tttgtgaggg cccaggacac
    53281 ggctccggcg atcaccttcg agcccagcgt gctgtgggtg tttcccgcct tctatggctt
    53341 ctggatcgtc atggtgctcc tcgccctgat cgaagcccgc aaggcccagt gggctgtcct
    53401 gatactcggc agcctgcttg tcatcctcaa tacgcttgga ggaatcagcg acgggttgcg
    53461 cgacggctgg tacgtcgcct tctcggcggt tttcttcatc acgctacccg gcgtgttcgc
    53521 catcgccgcc agttggcgtc atctcaacag caaaggaacc caccatgtcg atgaatagcg
    53581 ctgacttccc cctagtctgg acgagtctcg cccaagagcc cggacatgat caccagaaag
    53641 acttcgacga gttcgcggcc aacctgcagc gcggcgagcc gttcgtgctg ctgagcgata
    53701 cgctaccaag cgaagatcat gagcactcgc cggaagaaaa gaaacgcacc gcgctctgga
    53761 tgaagaagca caaagcgcag ttacgcagcc tggttctggc cctgatcatg gtcgaaccca
    53821 gctcggcaaa gcgcctgggc ttcaaggcgt ttgcaggcct tttcgccaag gtctggggct
    53881 accctctgct gtttgccgct tcacgtgagc aggccataga gatggcaaat gaactactcc
    53941 gaaaggataa agatcagttc cactgaaccg ctccagtttt taaatcaatg gggggtagag
    54001 tcatagatcc actcgggtat gcgggagaca gaaagtcaaa atcaatgaca ctgagcctat
    54061 tgattacgcc agtattttcc caagggcagc gacttcagca agctgatcgt ggaggcggtt
    54121 aatcgagtgg tcgcgaagat caatttacgc caactccccg caaacgcctg ggttggaaga
    54181 cgccgtatga ggtatatgct ggggtgagcg ttgcacttat gggttgaatt caagcttgat
    54241 aaaactgcat ggccagccat ggcgcatccc tttacgatca ggcaactacg gggccggaag
    54301 tatctccatg gactgtcaga ttagatacgc aacacgcgat gatgttgctg ccatcagccg
    54361 ggtcgtcgtg agtgccttga gggcatctaa tgcgcaagac tacccgccag atgtcatcga
    54421 gcgggtggag caaggatttt cccccgaggc gatccttcac ctgctgaccc agcggcaaat
    54481 atatgtggcg acagtcgatg cccatgtcgt ggcaacggcc agccttgagc gggacatggt
    54541 gcgaagcgta ttcgttgcgc cggcctatca gggtgcaggc ataggcaggc agttgatggc
    54601 ggtgatccag ttagtcgctg ccagcgaagg catcagtctg ttgcgtgtgc cgtcatcaat
    54661 tactgcagaa gacttttacg catcgcttgg ttttaaacag cttcgcgacg agtttcacgg
    54721 cgcagagcgc acgatcatta tggaaaagcg cctggatcaa taacgtctct cggcgcattc
    54781 aaaacgtctc gcttgtttgg tacacacacc tgccaattgc gaaccgaccc aggcagttgt
    54841 tccgctatat ccgtcgttct cggcggtacg tccgccaccc tcgctcccgg tgttcaatac
    54901 gccacgggcg ttcgataagc agtagtttca cttcggaagc aatcggtata gcaatcgggg
    54961 acagaccacg atattgatgc tgccgaccgt catcatgtgg acgactctgc ctgtcgtgac
    55021 tgaccgcttt tggccgcttt ctgccagtca agcgataaag cctgctggtc aggtcaaacg
    55081 caatcgttgg tcagcacaaa cgcaactgac tgatcagttg cgtgcaatta cgcaggcagg
    55141 ccgcgcactg gtgagtccag ccttataacg ctcggttgag ttgtatctca gcggtagcga
    55201 gccagtatgc cgtcgaattc accagcctgc ttcatctgca ccatggcgcg cagcagggcc
    55261 atggtgggca catccggcgc gtcgcggacg atgcaggcaa tcgggtctga ggccacttcg
    55321 ctaaggaggt gcagtttctg cccgggcggc tggtggcggt tgaaccagtg cagcgagagt
    55381 tcgttgctga tggcgtagct gtagcgccgg gcgctgagtt tgagcagcac ctgatcctgg
    55441 gtgcgggcgt cttcgcgttg gatctgcccg ctggcgaaca gaggctcaag gcgcgggtaa
    55501 gtgaagccga gcaccgtgcc taggcgttca ttgagcagtt gttcgggttc cagcttctcg
    55561 ggagtaatgc ctgccagcac atcgcgctgg gtcataaagg gcaggctcca gatatagcga
    55621 tagtggccgc tgttgatcca attcgggctg acgtagcagc gcacatcgat ctcgccggta
    55681 tccagcgctt gttgcacgcg taagcgcggc atcactacca gctcagcctt gcgcccgacc
    55741 ttggcggcca tgcgcagttg caagtcgacc agaatgcctt ccacggcctt gccgtcttcg
    55801 atacgcacca tgggcatggc ccagctgtcg ttgatcgaga agcgcagcgc ccgttcgctg
    55861 gcgaatgatg cgcagctcag ggtaagcaat agcagggcag gcaacagctt gtgcaccagg
    55921 gcagatcctt tgcgctgtag gcgcttcagc gggtcatcgg cagggccgcg cccatcagtg
    55981 caaacattcc gccgcagcaa cggttgaagc ctttgccagt gcgttgcagc cagggccgca
    56041 cgcgatgagc catacgcgcc agcaggtatt cgacgccgcc ttcgaccact gcaaaggtca
    56101 gcgccatgat cgcgaactgc agccagaggt caccgcgcgg gtcgagaaac tgcggcagaa
    56161 aggcgccgaa aaacaggatc accttgggat tggacagcgc cgacagcagg ccctggcgaa
    56221 atagcttgaa gccgttgctc tcggtggctt tctcggccat cgcgaggtgc agcggcggcg
    56281 ctcgccagag ttgaataccc agccagatca ggtaggcgcc gccgagccat ttaaggatgg
    56341 tcaaagcctg cgccgaggct tgcagcaggg tgccgatacc gagcatcgac aaggccatca
    56401 gcagggtaaa acccagcacg ccgccgctga cggtaaacag ggttttgcga tggccgtaca
    56461 aggcgccatg gctgagtgcg agcagtccgt tggggccagg cgtcagcgac aggccaaata
    56521 ctgcggtaag aaagatcagc caggtatgca gagccataaa gcaggtatcc agaagcggga
    56581 gagtgcaggg cagagcctgc gtgcctctgt tttttcatgc gctgactgga tagtcaagcg
    56641 gccataagtc gcggtggcgg atatgcagat gcagcggtcc tgcccgcgaa gacaggaccg
    56701 gcgctatcag cggtgggttg ctgctgaatg accacaggta gtgccacacg gctgcgttgt
    56761 gcttctcggt caattgcgtg gcgccgaagt accaaggctt gagctagctg aaagcggtca
    56821 tgttcatcag aagccgcttg cgcatggcaa aaacctttct tgttattagg gaaaggcgag
    56881 catatagatt gtccgacaat cagctatcac ccggacgggt gaggctgctg catcggcgct
    56941 caagcgccgg ctcgcggggc ctgcgggatg tgtcttgctc cacgttgtga gcactttccc
    57001 tcaatagccg acagcttgtt gcagttgtcc tgacgtgctg agcggacgaa gaccctctgg
    57061 ctgtggcaaa ctccgtagct cgtaacagga cgtttcatgg atcgagtttg ctgagcctgt
    57121 cagcggtttt tttgcccatg gcctagcctg ccctgggcgt attgcataac tggccgctgc
    57181 ctgcggcgaa gaacagagga atgactgtgc ataacgtcgt gatcagtggt accggcctct
    57241 ataccccggc aaacagtatt tccaacgatg agttggtcga gtcgtttaac gccttcgtcc
    57301 agcaattcaa tgccgacaac gccgcggcca ttgccagcgg tgaagtcgaa gcgctgagcg
    57361 aatcgagcag cggctttatc gaaaaagcct cgggcatcaa gagtcgcttt gtcatggaca
    57421 aggccggcat cctcgacccc aagcgcatgg cgccgcgcat cccggagcgc agcaatgacg
    57481 actggggcat cctctgcgag atggccgtag gcgccgccaa ggacgccatg gcccgcgccg
    57541 gcaagacagc cgccgatatc gatggagtga tcgttgcctg ctccaacctg cagcgcgcct
    57601 acccggccgt ggcgattgaa gtgcaggccg cgctgggcat ccagggtttt ggctacgaca
    57661 tgaacgtcgc ttgctcctcg gccaccttcg gtatccaagc tgcggccaac agcgtgcaac
    57721 tgggccaggc ccgcgcgatc ctgatggtca atccggaaat ctgtaccggc cacctgaact
    57781 tccgtgaccg cgacagccac tttatcttcg gtgacgccgc cacggcggtg attatcgagc
    57841 gagccgacca ggctacctcg aactaccagt tcgatatcgt cggcaccaag ttgatcaccc
    57901 agttcagcaa caacatccgc aacaacttcg gctttctcaa ccgctgtgcg gaagagggcg
    57961 ttggcgcgcg tgacaagctg ttcgtccagg aaggccgcaa ggtgttcaag gacgtctgcc
    58021 cgatggttgc cgagctgatt gccgcgcacc tggccgagaa caagctgaac gtcggcgacg
    58081 tgaagcgttt ctggctgcac caggccaacc tcaatatgaa cctgctgatc gcgcgcaagc
    58141 tgctcggccg cgatgccgag ccaggcgaag cgccggtgat tctcgacacc tacggcaaca
    58201 ccagttcggc cggttcggta atcgccctgc acaaatacca ggcagacctg ccaagcggcg
    58261 cactgggcgt gctcagctcg ttcggcgcgg gctattcgat tggtagcgtg atcctccgta
    58321 agcactgatg gattaccccg ctgatcaggc gctgctcact cgcctgcgtg caggcgagca
    58381 gaaggcctac cgcgagctgg tcgccaccta tcaaggggcg atgcgtgcgg tggcctttgc
    58441 cattgttggc agccgtaatg ccgatgaagt ggtgcaggac gcctggttgg ccgtagtgcg
    58501 cagcctcgat ggttttcagg cgcgttccag cctgaagact tggctgctga ccatcaccgc
    58561 caataccgcg aaaacccgcc tcaagggcaa tcgccgtgaa gtgctactcg acgatattcc
    58621 ctcgcctcac ggcagcattg gtgatgaacg cttcgcttca gatggccatt ggctgctggc
    58681 gccgcatgcc tggcatcacg actccccgga agcgctgctg accgaggacg aattgcgtga
    58741 atgtctggag aaaaccctcg ccagcctgtc tgagctgcag ggcagcgtgt tgcacctgcg
    58801 tgagcgcgaa gggctggagt tggaggagat ctgtaatctt ttgcaggtct cgctctccaa
    58861 tgtgcgggtg ctgctgcacc gcgcgcgtct caaggtattc gccaccctgg agcattttga
    58921 ggagaccggc caatgttgag ttgtaaggag ttggtggccc actccagcga atttcttgat
    58981 ggtcagctgg gcctacgcaa gcgcatgtcg gtgcgtttgc acctggccat gtgtcagcac
    59041 tgccggcgct ttatccggca gatgcgcctg agccaggcgg tgctacgcca attgccgccc
    59101 gggaagagcg ccgagctgga tgccttggct gccaagctgg ccgaattgcg ccgcagcaac
    59161 tcctgatcgc caggccattg gcctggctca cttcccgctt tattcggctt cttgcgcggc
    59221 ctttagcgct tcataggccg gagcggcgta aatgccgact tcgactgcca gctccatcac
    59281 ccgcgccagg tcagtgctgt acaccagcac ggcttgcagc tcgtcatcca gcggttcgct
    59341 gaaatccagt aacacataac ccttggtttc cggcgacagg ctggacagtt gcacctggat
    59401 gcggtttagc gctttcagcg gctcgacttt cagcaactgc ttgggcttga gcacaaagct
    59461 tacttccggg ccgaacgact cggtgatctg ctggaacagc gcgtcataac tgtcggcctg
    59521 gaacagcacg gtttccggca ggctgccgac caccacccat tcgcccagcg gaatcgggaa
    59581 gctgtcgtcg aagttgatat ccgggttggc ggcgagaaac gcctgcgggt cggtataggc
    59641 ctgggccgcc tcggtggcga tgcgcgcgac ttcttccggc agcatgcagc ccgagcttat
    59701 tttgctgatc agttcttcga gctgggcttt cattcgggtc atcctgcggc agaaaagagg
    59761 ccggcaagga tagccgcaaa tggctgcaga agctgcatgt cggcctattt atggcctgca
    59821 cgaaggccag tgtatcgctg cttgtaacgg ctcggcatag aggctgtctg tacgctaccg
    59881 tacagatata aaaacagatt ggattatgct ggcgtgtgca gttaaggctc tgtagcgagc
    59941 gcatatcaat cgccaacagg ccttagaaga ccggagacag tgatgtccgc agccccccat
    60001 ccgcagcaaa accacctgct tgccgcctta cctaacgaag tgctggagcg cctgttgccg
    60061 caccttgagc tggtggatat gccgctcggt atggtgctct acgaatcggg cgacaccctg
    60121 cggcatgtgt atttccccac cgactcgatt gtgtccctgc tgtatgtgat ggaaagcggc
    60181 gcctcggcgg aaatttctgt ggtgggtaat gatggggtga ttggcgtagc gctgtttatg
    60241 ggcggcgaaa gcaccaccag tcgcgccttg gtgcaaagtg ccggcagcgc ctatcgggtg
    60301 ctcggcaaac gcttcaagga agagttcaac cgccatggcg acatgctgca gctgatgctg
    60361 cgttacagcc aggcgctgat tacccagatg gcacagacgg cggtgtgcaa ccgccatcat
    60421 tcgattgacc agcagctgtg tcgctggttg ctgctgtcgc tggaccgact gaccggcagc
    60481 aagctggcca tgacccagga gctgatcgcc aatatgcttg gcgtgcgccg cgagggcgtc
    60541 accgaagcgg ctggcaaact gcatcgtctg ggtgtgatcg attacagccg tgggctgatc
    60601 accgtgttgg atcgggacaa gctcgaacgc ctgagctgcg agtgctatgc cgtggtcaag
    60661 cgcgaaaccg accgcctatt gccttataaa gtggcgcttt cggcgcacta atctgttacg
    60721 cgcgcggtta gcaatttagt ggcttggctt gagtgccgta gcgcacagag ctttgccaga
    60781 tgctcaggca gactgcagcg gcgatgacgt caggcacagt tgtcgtgcct ttttttggca
    60841 agcgtcgtgc tcgagacaag taaatgtctg ttaaagcacc gctgcatatt ctcagccgtc
    60901 tgatcgaagg gctgccggtc aaacaacgca aacaactcct cgatggctgc gagccggtgg
    60961 acctggtttt cggcaatgtg ctgcatgagg ccaaccagcc gattcgccat gtgtactttc
    61021 ccctgagcgg ctttatctca ctggtcacca ccctggatgg ccatcaacct ctggaaatgg
    61081 gcctgatcgg caatgaaggc atgctcggcg caacgctcgc cctgggcata ggccaggcac
    61141 ccatgcgtgc ggtggtgcaa ggttcgggca ctgccttgcg catcagcagt cagttgttca
    61201 agcaggagtt gctcagcagc ccggcgctgt tgcgtgtgct caaacgctac ctgtatgtgg
    61261 tgatggcgca gttgtcacag agcgcggcct gcacgcgctt ccatgcgatt gagccgcgcc
    61321 tggcacgctg gttgttgatg acccacgacc gtgcccacgc cgatcatttc cacctgaccc
    61381 acgagtttct tgccgacatg ctcggggtac ggcgcagtgg cgtatcgatt gccgccgcgg
    61441 ccatgcaggc gcgcggtttg atcagctata gccgcggtga gatcaaaatc cttgatcgcg
    61501 ccgggctgga gcaggccgcc tgcgagtgtt atgccgcgct gcaggctgac taccacgcgc
    61561 agttctagcg ccaaggttgt tccttgcgct tagcaactta gcggaatagg cctcaggcgc
    61621 tggttgcctc ataaagcgta tggattggcg agctgaggcc gatgcgcagc aggtcgccga
    61681 agctttctgc gctgaccggg tggctgaaca gaaagccctg gccttcggcg cagtggtgat
    61741 ggcggagaaa ctccagctgc gcggtttctt cgatgccttc ggcgatcacc cgttgtttca
    61801 ggctgttgcc catggcgatc accgcgctaa cgatgatgcc gttgctgttg tgaatgtcct
    61861 gaacaaagga gcgatcaatt ttcagcacat cgatgggaaa ctggttgagg taactgaggc
    61921 tggagtaacc ggtgccgaag tcatccaccg ccagctgcac gccgaggatc ttcagttgat
    61981 ggagaatcgt cgcgctggcc tgggcgtcgc gcatcagcac gctttcggtg atttccagtt
    62041 gcagcctgtc gggcgcaagg ccgctggcgg tcagggcatt ggctacgctg ccgacaaaat
    62101 ccttgcggcg aaactccatg gacgagatgt tgaccgccac cgaatccagg ttgtagccgc
    62161 agttctccca gcgcttggcc tgggcgcacg cttgcagcat cacccagcgg cccagcggca
    62221 ggatcagacc gcaggcctcg gccaccggaa taaagcgcgc tggtaggacc atgccccaga
    62281 ccgggtgctg ccagcgaatc agcgcttcgg cgctggtgat tctgccgctt tgcaggtcga
    62341 tcttcggctg gtaatgcagg acgaattcgt tgttctccag ggccgtgcgc aggtgtgctt
    62401 cgatcagttg gcgttcgacc gcgtgaatat tcatctcatg acggaagaac tggtagttgt
    62461 tgcgaccttc ttccttggcc tggtacatcg ccgtgtcggc atttttgatc aggctttcgg
    62521 catccttggc atcgttgggg taggtgctga taccgatgct ggaggtgaca tgcagctgct
    62581 ggccaatgtt gtggggcatg gccagggtat tgagaatttt ctcggcggtg agggtggcat
    62641 cttcgtccgt ctggttttcg ctgagcagca cgacaaattc gtcaccgccc aggcggctga
    62701 ccgtgtcggc gctgcgcacg cagtcgctca ggcgcagcgc caccgcttgc agcagctggt
    62761 cgccgacgcc gtggccgagc gagtcgttga tgtgcttgaa gttatccaga tcgagaaaca
    62821 gcaatgccag gctgctgcca ctgcgttcgg ccagggcaat ggcctggttg atccgatcat
    62881 taagcagggc gcggttaggc aggttggtca gggcgtcgtg ctgcgccagg tgagccatct
    62941 tctcggtcat cgcctgggcg gcggtcatgt catggaagac catcacggca cctttgatgc
    63001 gctcggcgga atcgtgaatc ggtgcggcgg agtcctcaat ggcggcctcg ctgccatcgc
    63061 ggcgaatcag gatagtgccg gcggccagca gtttggcttc atcctgttgc agcaccttga
    63121 agatcgggtt gggcactgtt tcgcgggttt tgtcgttgac caggcgcatc acttcgtcga
    63181 taggccgacc ctgagcttcc tcaagtaacc agccggtcat ctgctcggca gcgttattca
    63241 ggtagtcgac gttgccctgc agatcggtgc tgatcaccgc gtcactgatc gagttgagcg
    63301 tcagcagcgc gcgggctttt tcgacgaaca gcgcttcttc cacggccttg cgctggatga
    63361 tattgcgcag cgattgtggc accaggtaac tgctgaagtg acccttggac aggcagccct
    63421 gagcgccgcg ctggatggcg tcccaggttt gctggtcatc ctccagggca cttagggtca
    63481 ggattggaat gctcgaagcc agggcaaaca gctgatcgaa ggtgtccatg ccttggctgt
    63541 ccggtaggtc gaggtcgacc atgatcgcgt caatgggctc tttgcgatgg gtttccaagc
    63601 gctctagggc gcgcgccagg ctggtgaccc actcgatcag gaaagggccg tcctgggccc
    63661 tgtttagtac gtcttccagg gtggctgcgt cgtcggtatt gccggtaata atcaggacgc
    63721 gattagccat gatgcctgtt cttccttgcg caacattacg cagggcaggt aaggcactgc
    63781 gttgccgata cggcatttat catcgttgga ggtgcggtgt tgctgggcac tcagaaacgc
    63841 agccccgacg gcggcgattg gtcgtgctga ggtgctttaa cgggcaggct gatccagcgg
    63901 ctgtttagac gcacggtgct cctggcaagt tcagtgtggt ctttttcagg ttgcagcctg
    63961 gcgttgtgta gattgctgct gcgcgccgcc tcggcggttg tcgcgggctg cggcgcgcgg
    64021 ctgaatactt ccggcgcaaa tagctcggct gatgcgttgc cggccagcaa agccagcagt
    64081 atcagcgcga tgctgctggg cagcgcgtga gtgtgtccaa gcgtgtgcat gtcatctatc
    64141 cctgtttgtg gacgtgtttc acgtgccttt ccagcacaca acgttcggcc gcaggctttg
    64201 gcccgcgctc acgtcgacaa catgcgtgta tgggtgttgt ttgttatgtg cggttgttgc
    64261 tcagggaggc gccgtgcggt ctgtggctga ggctgaggct gaggctggcc gtgtgggcgt
    64321 agaggcaagg tggcgctggg tggctatgtc cataatgacc tctgagcaaa tcccacgcgg
    64381 gcgcaaatac cgggggcgaa gcttttagat acatggcttg ccagggtgtt gtcggtgcgc
    64441 tagcgcacct agcgtgcata cgggctaaag cctgcgcggt ttggccggtt gggctgctaa
    64501 actggcgcac tttgcaccgc ccgatcactt tttggaggct gtcgtgagcc cgcgtattca
    64561 ccctttggcc ggtcagccgg cacctgcttc cctgttgacc gatatctctg cgctgctggc
    64621 ggcttattac gatctgcagc ctgatctgag cgtggccagc cagcgcgtgg cattcggcac
    64681 ctccgggcat cgcggcagct ccctgaccca cagtttcaac caggcgcatg tgttggcgat
    64741 cagtcaggcg atctgtgatt accgggtggc ccagggcatc agcggcccgc tgtttatcgg
    64801 cgccgatacc catgcgctgt cggcgcctgc gcttgacagc gcgattgaag tgctggcggc
    64861 caatggcgtg cagaccatga tctccactgg cggtgagttc accccgacgc cagcgatcag
    64921 tcaggccatc ctggtgcaca accagggccg cagttcgggc ctggccgatg gcatcgtcat
    64981 caccccctcg cacaacccgc cggacagcgg cggcttcaag tacaacccta ccaacggcgg
    65041 cccggctgac agcgatatca ccacctgggt gcagaaccgc gccaatgcgt tgcttgaagg
    65101 cggcctgcgt gaggtcaagc gcatgcccct ggcgcaggcg cggcaggcgg ccaatgtaca
    65161 ggaatacgat tacctcggca gctatgtgag tgacttgggc aatgtcatcg atttcgcccc
    65221 gatccgtgcc gctggcctgc gcctgggtgt cgacccgctg ggcggcgccg gggtgcatta
    65281 ctgggcgcgc atcgccgagc agtacggtct caatctggat gtggtgagcg agattatcga
    65341 tccgcagttc gcctttatga gcctggactg ggacgggcaa atccgcatgg atccatcctc
    65401 cagccatgcc atgcagcgcc tgatcggcct gaaaaacggt tacgacatcg cctttgcctg
    65461 cgacaccgac tacgaccgtc acggaatcgt tgcgccgagc gttggcctgt tgccggccaa
    65521 ccacttcctc agcgtggcca tcgattacct gttccagcat cgcccgcagt ggagcgccac
    65581 ggcggcggta ggcaaaaccc tggtcagcag cgcgatgatt gatcgtgtcg ctgcacgtct
    65641 gggccggccg ttgcaggaag tgccagtggg tttcaaatgg tttgccggcg gcctgttcga
    65701 cggctccctg ggctttggcg gcgaggagag cgccggggcg accttcttgc gccgcgatgg
    65761 ccgggtctgg accaccgaca aggacggtat cgcgttggcc ctgctggctg ccgagatgac
    65821 cgcagtgact gggcgtgatc ctggcgagct gtatcagggc ctgagcgatg agttcggcgc
    65881 gatttatgcc gaccgcgtcg atgcaccggc cacacctgcg cagaaaaaag cgctgggcaa
    65941 actctcgccc gcgcaaatca gcagccgcga gctggccggt gatgccatta cccaggtgct
    66001 cgacaaggca ccgggtaatg gcgcggcgat tggcggcatc aaggtaatca gcgagggcgg
    66061 ctggtttgcc gcgcggccgt ctggcactga ggacatctac aaaatctacg ccgagagctt
    66121 tcgcgatgcg gcgcacctgc agcgcatctt tagcgaggcg cagcagatcg ttgatggggc
    66181 actggtcgcc gaataggccg cagcgtttcg gttcgctagc gcaccgactg caactggcgt
    66241 ggcgtgcagg atgatggagc gcttgggtgc tattccggta cctgctgttc cggcgcactg
    66301 cagtgactca tcaaggagaa cgccatgagc ctgggtacca ttctattgat cgtgctggtg
    66361 ctgttattga tcggcgccat accggcctgg ccacacagca gaagctgggg ttacgggcct
    66421 acgggcggtt tgggcgtagt ggtggtggtg ctattggtgc tgctattaat gggccgaata
    66481 taaccgctac ctgacatgtc ccacggccgc tgcgcagcaa gtggtttgcc gctttcgcct
    66541 gagtgaatca ggcgttagtc tgcgcagcgt atccccgctg tggtcgccgc atgtaccgtc
    66601 ttgatctgaa aatccgccta cataacggtg ctgatattgc catcgggccc ggcaaggccg
    66661 agctgttgtc ggccatcgcc cagcacggtt cgatcagcgc cgccgcgcgc gccatgggca
    66721 tgtcctatcg gcgtgcctgg ctgttggtgg aaaccatgaa ccgcagcttc aaacagccgc
    66781 tggtcagcac cctggtcggc ggcaagcagg gcggtggcac tcagctcacc gccaccggtg
    66841 agctggttct gcagcgctac cgcgctatct gcgccgccgc catggcagcg gtgcaaagtg
    66901 gttgtgacga gctggccgag ctgctggccg agccacccgc caacaacgtc ctataactcg
    66961 atccgcaagc gatgcgccgc gccatccagg gccactttca atgacccttg cgagcagtcg
    67021 atgggctggt cgtccagcca ggcctggcga atgccctggc tgcgttgcgc cgggttgctc
    67081 agctcgatct ggtagtggct gccggccagg gcgatgcgtg cgctaaagcc tggccagtcg
    67141 gccgggatgc aggggttgag cagcagccag tccccctcgc ggcgaatacc gagaatccct
    67201 tcgacgccgg cgcggtacat ccagccggcc gccccggtgt accaggtcca gccgccccga
    67261 ccgttgtgcg gcgccaccgc gtaaacatcg gcggccagca catagggctc gaccttgtag
    67321 cgctggcagg cctcagggct gttgccgtgg ttgatcgggt tgaccaggtc aaacagggca
    67381 aaggcctgct gcgcggaacc gagctggcaa tacgccagaa tcgcccacat cgccgcgtgg
    67441 ctgtattgcc cgccgttttc gcgcaggccc ggtgggtaac ccttgatata gccggggtcc
    67501 aggtgcgttt tgtcgaacgg cggggtgaac agcagggcga tgccgtcgtc ctcgcggatc
    67561 aactggcgtt gcatggaggc catcgccatg ttcacccgca gcggatcggc gctggccgac
    67621 agcactgccc aggactgggc aattgagtcg atccggcact cgctgctttg caccgagccg
    67681 agccaggtgc cgtcatcgaa ggtcgcgcgg cggtaccact tgccatccca ggcataggtc
    67741 tccagcgcca agcgcagctg ctcggcatgc gctcgccagc gtgctgcgcg ggcgctgtca
    67801 tacacctcgg catagggcgc gaacagcgcg atggtgcgca gcagcaacca gcccagccac
    67861 acgctttcgc ctttgccgtg ctcgcccacg cggttcatgc cgtcgttcca gtcgccgccg
    67921 ccaatcagcg gcaggcccag ctcgccggtc agttgcaggc attggtcgag cccacgggcg
    67981 cagtgctcga acagcgaagc cggcgcatcg gcctgcatcg gctggaagaa cgcatcgtgc
    68041 tcgccggttg ccagcagcgg gccttcgaga aagtccaact gctcattgag aatcgccgca
    68101 tcgccgctgc actggatgta ctgcgcggtg gcgtaggcca gccacacccg gtcatcggag
    68161 atgcgcgtgc gcacgccctg gccggaatgc ggcagccacc agtgctgcac atcgccttcg
    68221 acaaactggc ggctggccgc gcgcaggata tgcccgcggg ttatcgacgg gctggcgaag
    68281 gtcagcgcca tgccgtcctg cagctgatcg cggaagccgt aggcgccact ggcctgatag
    68341 aacgccgagc gcgcccagac gcggcaggcc agggtctgat agagcagcca gccgttgagc
    68401 atgatgtcca tggcgcggct tggcgtctgc acttgcaccg cgccgagtac gcgctgccag
    68461 tacgcttgca ccttgagtag cacggcgtcc agatcggcct cgcggtactg cgtaatcaag
    68521 gcgctggcct tctgttcgct ggcgcattgg ccgaggagcc agagtacctc cacgctttcc
    68581 cctggttgca gttccagctg gcattgcagc gcggtgcagg ggtcgaggcc cgcaccgagg
    68641 ctgccgctca gtggtgccag gcgggcgagg gcggcgggct gtgtgaggtt gccgttacgc
    68701 ccgataaact cgctgcgatc ggccgtccag ctattttgct ggccgcccag gtcgctgaac
    68761 gcgttgcgcc cggtaaaggc gatgctccac gggttgcgaa ccaggatggc gccggtggtg
    68821 ctgtcctgct ggctgacgat aaatggcgcg ctggccgccc gcgtgctacc cagcacccat
    68881 tcggcatagg cagtaacgct taggcggcgt ggccggccgg agaggttgcg cagggtcagc
    68941 cgagaaatct tgatcgggtc ttccagcggc acgtattgca ataattccat ggctatgccg
    69001 ccggcctggt ggctgaagcg gctgtagcca aagccatggc gggcttcgta gtaaccgctg
    69061 tcgcgcagcg gttgggcagt ggggctgaac agcgccaggc tgtcttcgtc gcgcacatac
    69121 agggcttcgc ccggtgggtc gctgaccggg tcgttggacc agggcgtgag ctggttttcc
    69181 cggctgttgt cggcccaggt gtagccgctg ccttcggccg aggcctgaaa gccgaactgc
    69241 ggattggcga tcacgttgat ccacggcgcc ggggtgttgc tccaggcatc caggcgcacc
    69301 acgtattcgc ggccatccag gtcgaaaccg cccaggccgt tggcaaactc cagcggactg
    69361 ggcggcggcg cgtggaatac cgggatgggc tgcgcactgt ggttgctggc gcttggcggg
    69421 acggggctgg ggcagggcgg aatacgcgcc agttgctcgg aaatcggccc gcgatgggcc
    69481 accagcagca ctcgggcgac ggcctgcagg cgttcgcggt attcaccgct gagcaggtcg
    69541 gcgcgcaata cgaacaccga gccctgcgcc agttcggcgg caaagcgtgg tcgcgactga
    69601 ctgctgcgca cggcggtttc gatggccacc tgcagatcct gcaggtagga ggaggcatgc
    69661 tcgttgacga tcaccaggtc caccgccaga cgcttcatcc gccagtattc gtgggcgcgc
    69721 agcagctgtc ggacctgggc gatgtcttcg gcgtcgctga tgcgcagcag gacaatcggc
    69781 aggtcgccgg aaatcccctg ggaccacagc accgactggc taccgaggcc gcgcagcagg
    69841 ctggccggtg gtgcgcgaaa acgcgagtcg gcatacagga ttggcgcggc caggcgctga
    69901 aaatcggcgg cttcggcggc gagcatatcg aggtggcgca actccacctg ggcctgggtc
    69961 caggccaggg ttttcgcccg ctcgaaggcg ctgcggtcgt ggtgcttgtc gatcagatca
    70021 agcagcgcct cacgggagga ggcaaccact gtccagaacg ccacgcgggc gatcttgccc
    70081 ggctcgatca gcaggcgctg gcgcagtgag aagatcgggt cgagcaccgt gccaacacta
    70141 ttggacaggc gctggccctg gcgaattacc gcagcggctt gcagcgcgca actgcgcccg
    70201 agaaacttcg cgcggtcgga ctcgtactgc ggctcggcgc tcacttcacc ttcaaccacg
    70261 gcaaagtgcg cggcccacaa cgatggctcg ctggcactgc gcggacgccg tgtggcaatc
    70321 agtgcggcga aggcgggcag gtattcggtg accacaaaca gcttggagaa cgccgggtgg
    70381 gcgttgtcgc tggcggcgct ggcgagcacc agttcggcgt aggaggtcag ttcgatttcc
    70441 cgcgccacgc ggccgctgtt ggtcagcgaa acgcggcgca cttcaccgtc gtcttcgcct
    70501 gagaccagca cttccaggct ggtggtcagg ttgccgtcgt ggcggctgta gctggcgtag
    70561 tcctcggcga acaccacttc gtcatagggg ttgccgctgc tcggctgcgc gcccagggac
    70621 cagtgcgtac cgttgcgggt gtcccagaga aagataaagg agccgtgctg gtcgcgggtg
    70681 ctgtcttcct gccagcgggt aatggcgatg tcgcgccagc ggctgtaacc cgcgccactg
    70741 gcggtgagca tcacgctgta acggccgttg ctgagcaggt gggtaaccgg tgggttgcct
    70801 tctacatggt tcaggcgtcg ttggttgtgc tgctcgttgg tggtttccac ggcgcaggcg
    70861 ctgacctctt cggcgcgcgg gtgggcgact gcgacattgc gcggcatgcg ttcctgcaac
    70921 agcagctcgc aggcctggat catcggttca cggtggaaac gcgcacgcat ctggccgtca
    70981 tgcagggtgt tggcaatggc gacgatgctc atgccctggt ggtgagccat aaagttgcgc
    71041 acgatggcaa acggctgatc atccggcagg cgggtgcggg tgaagtccag cgcctcatag
    71101 aagccatagc ggcccagtgc gcccttagcg gccaggcggc ggtagttggc cagtgcgcct
    71161 tgcgggtcga ccatggtcgc caggcccgtg gcgtacgggg caatcacccg gttggcggac
    71221 aagccgcgct tgaggcccag gcccggtacg ccgaagttcg aatactggta ggtctgctcc
    71281 atatcgcggg cgttgtaggc cgattctgaa atcccccagg ggatgcccaa cgcttcgcca
    71341 taggcagcct ggcgcgccac caccaggcgg ttggtctgct ccagcaggct gccggccggg
    71401 gcgcgcatta ccagtgaagg catcaggtac tcgaacatcg agccggacca ggaaatcagc
    71461 gccgagccgc tgcccagcgg ggtggccgtg cggcccaggc gaaaccagtg gcgggtgctg
    71521 acatcgccct tggcaatggc aaacaggctg gccaggcgcg cttccgaggc gagcaggtca
    71581 tagcagctgc tgtcgaggct gttgtcggcc aggcagaagc caatcgacag cagtttgcgc
    71641 tccggctcca gcaggaaggc aaagtccatg gccagggcca tgccgcgtgc ctgctcggcg
    71701 acgctgcgca gctcgtcgtg cagggttgtg cgttggctat ccgtcagctc ggcatcctgc
    71761 tgcagatcac caagggcctg ggtgagggcc tgcagccaga acagcagttc gccggcctga
    71821 ttattcgtgt cgagtggggc gacgccgctg gacagctgcg tggctttgtc actcaggcgc
    71881 agaagggttg gcaagaggct gtgcagcggc tgcgtaccat tcagggctgc ttcgatctgc
    71941 tgcagtaagt cgtgcagctg ttgactgcgt tcgctactgg cagccggcag ggccgccagc
    72001 gcggcgcagg ccagttgcag gttgtcgtgg atgccctggc gggcgtccgc actcacgggg
    72061 gctttcagcc attcctcgca ggcattggcg accaccagca ggtggccggc taggttgccg
    72121 ctgtccacgg atgacacata ggccggcgcc aatggacgca ggtcctgggt gtcgtaccag
    72181 ttgaagaagt gcccgcgatg gcgctgcagt tgctccatga ccgccagggt ggcctgcagg
    72241 cgggtgaggg tgcgctgggt gccggcccag ccgaaatcgc gtgcggcgat ggcggagagc
    72301 aggtacaggc ccatattggt cggcgaggtg cggtgggcga taaccggctt ggggtcttcc
    72361 tgaaaattat ccggcggcag ctggttgtcg gcttgggtga cgaaggtttc gaagaagcgc
    72421 caggtgcgcc gggcaatcag ccgtagctcg ctggtttgct ctgggtcgac cagcaggcgt
    72481 ttggctacgc gcggcgaacg gctaacccgc agggcaatcg ccggcgcgct aagccatagc
    72541 agggcgaacg gcagcaccag cggccagacg gccggcgacc agcgcacggc cagagcgcac
    72601 agcagtaggc cgagtacggt gccgccagcc atgccgcagt aaaagcccag cagcttcaga
    72661 cgcgggctga tcgatgcctg ggcggcggtg gtccactcca gcaggttgcg ccgggtgatc
    72721 agcaggcggg tcagggtgcg gctgaccgca tcgagcatgc gccagctctg gtcggcgagg
    72781 aacgtcaggt tcagcgcgca ttgcagcagg gccagtagca ggtcatcgcg cagcttgtcg
    72841 aggtggctgc gtaagcgaat accggcgcgg gccggtagca agccgaagca cagcggtaac
    72901 aaggcgggga tcagcaggct ggcaatcagc aacaggctgg cgctcagcgc cgccggcaac
    72961 ggcagcagcc agctcaggcc cagcgccgcc agggtggccg gggccagcag ggagcggcgc
    73021 aggttgtcga gcatcttcca gcggccgatg ctcggcagcg cctgtttgcc gctaaacggc
    73081 ccgagaatcc acggcagcag ctgccagtcg ccacgggtcc agcggtgctg gcgcttgccg
    73141 gctacgtcat agcgcgaggg gaattcttcg accacttcca catccgaggc caggcctgcg
    73201 cgggcgaaaa cgccttcgaa caggtcatgg ctgagcatgc tgttttctgt cacccggccg
    73261 gctagcgcgg cctcgaaggc atcgatgtca tagatgccct tgccggtaaa ggagccttcg
    73321 ccgaacaggt cctggtacac atcggacacg gccgcagcat aggggtccat gccgccgggg
    73381 ctggagaaga ccttctggta cagcgagcct tcgcgctgta agggcagtga cggggtaacc
    73441 cgcggctgca gaattgcgta gccgccgacc acccgctgca gcgctgtgtc gaagcgtggc
    73501 tggttcagtg ggtgggccat cttgccgatc aggcgcagcg ccgcatcacg cggcaggcgg
    73561 gtgtcggcat ccagggtgat gacatagcgc acatcggccg gtacgcgctg gcttagcccc
    73621 ggcacctcgg taaagctggt gtcagtggcg ccacgcagca ggcggttgag ttcatgcagc
    73681 ttgccgcgct tgcgctccca gcccatccag acgttttcac tgggattgaa ctggcggcga
    73741 cggtgcagca ggaggaaacg gctgccggcg atgccggggc cgtaacgccg gttgagctgg
    73801 tcgatggcgg tgctggccag ttgcagcagg gcgtcgtcgc tggccaggtg ttcctgctcg
    73861 gcatccaggc catcggtgag cagggcgaag gtcagatcgc cgccatgccc ggccaggtga
    73921 tgcacttcca ggcgctcgat ctgttccagc agatcagcct cgctaccgag cagggtcggc
    73981 acggcaatca gcgtacgcag attgctgggc accccttcgc tcagctccag gcccggcagg
    74041 cccatggcgc cgaagctgtt actgaccgca cggttgacca gcgcggtggc cacttcggtt
    74101 gccggtagaa agcccagcac acccagcgcc agcagccagc cggcagccac gccctggccg
    74161 gccaggctgt acagcgccag gcccagcagc aggctggcga ggccgacgat ggcgctgacg
    74221 taaccgggca tgccgaggcg gatatggcca cggctgatgc gcaggcgcag cggtgggcgg
    74281 aagttgatac gctgctccag cgccagacgg ccttcggcga tcaagtggta gccggggtca
    74341 gtggtgcgcg ggtcggcatg ctgggtgtcg cgggcctgtt gggtggcctg cagggcctgt
    74401 tcggcaactt ccagttcgct gcaggtgctg ccacgggcca gttgctcgat ggcgctgcgg
    74461 tactggttgc gggtggcgaa atccatcgcg gcaaagtcgc tgcgcgcgcc taggcgggca
    74521 tccaccaggc tgacgttctc gaacagctgc gcccagtcga tggaggagat cagacgcatg
    74581 ctgttgatga tattgcgcac cgtgaggttg gacgcgccct ggcgctgctg cgcgtgttgc
    74641 accacttcat cggtgctgct gccctgcgcg ccgaggcgcg cttccagcca accgagggcc
    74701 ggggtggtga gcgggtcgtg gtcacgcagg cgtttggcca gttgcgcggc aaagggctcg
    74761 ctcagcgctg tgcgggcgcg ctcgtgcaat tccttgtcga gcgccgcgtg gggcgcgtct
    74821 gggcccagca ggcggctggc cagggcgtcg gcgtcggccc gtgcgctgcg tccgacggtc
    74881 atctggtcgg cgaggcggcg gaggttctcg atcagcacga tgcgcagggt gatcgctacc
    74941 gcccacagtt cgccgatggt cagcggttgg acttgctgat aagcgacgat aaagcgctgc
    75001 aggatgtccg ggtcgaagtg gctgtcggtg tgggcgataa acgcccaggc catgcccagc
    75061 acccgtgggt agccggcgaa ggggccgctg gcgagtttcg gcaactggcg gtaatagccg
    75121 ggcggcaggt catcgcggat ctcgcgaatc tgctcttcga ccaggtggta gttgtccagc
    75181 agccactcgg ccgccggcac cacgccgcgg ccgttggcca gttcggcggc gctggcgcga
    75241 taggccgcga gcagcacctt ggcgttgtcg ttcagccggc gatgcaggga cagcacggcg
    75301 ggcggcttgc tgctcaccgg ctgggctgcg gccagggtgt aggcgtgttg ttcgaggcgt
    75361 tcgatgccga acagttcttc gcgtaccggg gcgtggtcaa accatggcga gggtgcagcg
    75421 gcacggctga atagagcaga gaagactgag gtcatagggc gcggttccag ccatcattgg
    75481 catgcgcgat aggcgtcgag gtgcgagacc catctgagaa accgcgacag cggacctacg
    75541 aagtcacgat tgccgtgccc ctgttgcttc gccgtgctga ttttgcatat tggccgcaga
    75601 cgctggtgtg accggtcaac acccaagacc cgggtaccgc gatggcgggg taaacctgca
    75661 ccatcgggaa gaatgctgtc gcagcattgt cgccacccta cagcacgctg ctggctggcg
    75721 tctgttcgct aacggacata gctgcaaatg ggccggcgtt taacgcagct gcgcttcggc
    75781 caaggtcagg ctgcgcggaa taagcgatac atcccaatgc tggctggccc agtgcagtag
    75841 ctcgccgggc tggccgtagt tggcgtcgtc cggtaacggc tggccaagcg cacgcagggc
    75901 gcggatcagc agtggggtgg cctggtcggc tggcagcggt ggcgaacggt aggatttgcc
    75961 cagcttgtgg ccatccggct ggatgatcag cggcacatgc aggtagcgcg gctgcggcag
    76021 gccgagcagc tcctgcaggt acagctggcg tggggtggag tcgagcaggt cggcgccgcg
    76081 cacaacgtcg gtcacgccct gccaggcatc gtcgagtacc accgccagct ggtaggcata
    76141 caggccatcg cggcggcgaa tcacgaagtc gcccacctcg cggcccaggt gctggcggta
    76201 atcgccctgc acgcggtcga caaagtggta ggtcagctcc ggcacgcgca ggcgaatcgc
    76261 ggcatcggca aaaccgtggc cggcgttgcg gcaggtgccg gggtagatgc cctgagtgcc
    76321 ttccagttgc ttgcgtgagc aggtgcaggc gtaggccaga ccctggctga gcaagcgctc
    76381 gaccagtgcg gcgtattcgg cgtggcgttc actttgtcgc gccagctcgc catcccactc
    76441 gaaaccgtag ttttccaggg tgctgagaat cccggcctga gcgcctgcga cttcgcgggg
    76501 cgggtcgagg tcttccatac gcagcagcca gcggccattg accgcgcgcg cgtcaaggta
    76561 ggacgcaagg gctgcgacca gtgagccgaa gtgcagataa ccgctggggg tgggggcgaa
    76621 gcggccgata taggcgggtg tgttcatttg ccgtggcaca cacagggaga cagaaacaac
    76681 agagggtgcc gaagcaccct ctgggtcaga tcaggagccg atctgttttt ctttgatttc
    76741 cgccagcgtc ttgcaatcga tgcacagggt ggcggtgggg cgggcttcga ggcggcggat
    76801 gccgatttct acgccgcagg aatcgcacca gccgtaatcg ttgtcttcga tcagttgcag
    76861 ggtttcgtcg atcttcttga tcagcttgcg ctcgcgatca cgggcacgca gttccaggct
    76921 gaattcttct tcctggctgg cgcgatccgc cggatcgggg aagttggctg cttcgtcctg
    76981 catatggtgc acagtacgat caacttcctg catcaattcc agcttccact tgttgaggat
    77041 gccggtgaag tgggcgcgca tcggctcacc catgtattcc tcaccctttg tttctttata
    77101 gggctcgaaa ccacgagtca gttggctgct ttttgctttt gctttggtgg gcatggatga
    77161 ccgcctctca ctcttctaat ccactgcgca ggacggtgtc ccttgcccgt tctacgtcga
    77221 gtagaactgg ccctgcggct gcaagcgggc gaacttacca gaacaactcg gcctgcgcca
    77281 ctcccggttg tcgtggttgc gccgggtatg ctttagaatc cgcggcttat ctcccacgca
    77341 gacagtccca tggcccagtc ctacagtgcg cgcagccgcg ccatagaacc tttttacgtg
    77401 atggcgctgt tggcccgtgc caatcagctg caggccgacg gccatgatgt gattcacctg
    77461 gaaatcggcg agccggactt caccaccgcc gcgccgattg tcgctgccgg gcaggcggct
    77521 ctggccaatg ggcatacccg ctacaccgcc gcgcgcggcc tgccggctct gcgtgaagcg
    77581 attgccggct tctacgcgca acgtcatcga ctaagcatag acccagagcg catcctgatt
    77641 acgcccggcg gttcgggcgc cttgctgctg gccaccagcc tgctggtcga ccccggcaag
    77701 cactggctgc tggccgatcc cggttacccc tgcaaccgcc actttctacg cctggtcgaa
    77761 ggcgcggcgc agctggtgcc ggttggcccg gatgtgcgtt atcagctgac gcccgacctt
    77821 atcgagcgct attgggatca ggacagcgtc ggcgcgctgg tggcatcgcc ggccaacccg
    77881 accggaacct tgcttagccg tgatgagctg ggcgggctgt cgcaggcact gaaggagcgt
    77941 ggcggccatc tggtggtgga tgagatctac cacggcctga cctacggcgt ggacgcggcc
    78001 agcgtgctgg aagtggacga cgacgccttt gtactcaata gtttttccaa gtatttcggc
    78061 atgaccggct ggcgcctggg ctggttggtg gcaccgccag ccgccgtgcc ggaactggag
    78121 aaactggcgc agaacctcta tatcagcgcg ccgagcatgg ctcagcatgc cgcgctggcg
    78181 tgcttccagc cgcagaccct ggacatcctc gaacagcgcc gccacgagtt ccagcgccgc
    78241 cgcgatttcc tgctgccggc gttgcgtgag ctgggcttca agatcgccgt ggagccagaa
    78301 ggcgccttct atctatatgc cgatatcagc gcctttggtg gtgatgcctt tgccttttgc
    78361 cagcactttc tggaaaccga gcatgtggcc tttacccccg gtctggattt cggtcgctat
    78421 caggccgcgc agcatgtgcg ctttgcctac acccaggacc tgccgcgcct gcagcaggcg
    78481 gtggagcgga tcaagcgtgg cctgatcagc tggcagtccc atgcagtttg atccgccgct
    78541 ggaggaaggg cggcttctgc gccgctataa gcgctttctc gccgatatcg agaccaccag
    78601 cggcgagctg ctgtgtattc actgccccaa taccggttcg atgctcaact gcatggccga
    78661 aaactcaaaa gtctggttta gccgcagcaa tgaccccaaa cgcaagctgc cgggcacctg
    78721 ggaaatcggc gaaacgcccc acggccgtct ggcggtgatc aacaccgggc gcgccaatgc
    78781 cctggtggag gaggcgttgc gcgccgggct gatcgctgag ctgaacggtt ttaccagtct
    78841 caaacgtgaa gtggcctacg ggcaggagcg cagccgtatc gactttcgtc tggactaccc
    78901 aacaggcgcg gcgtttgtcg aagttaagag cgttaccctg gggttcgaag gcattcgcgt
    78961 agccgccttt cccgacgcgc gcaccgagcg cggcagcaaa cacctgcgcg aactggctgt
    79021 attggcccgt gaagggatac gcgcggtgca gttgtattgc gtgaacctga cggacattga
    79081 tgccgtgcgc ccggccgagg aaatcgaccc ggcctacgcc gctgctttgc gtgaagcggt
    79141 gagcgccggt gtggaagtgc tggcttacgg cgcggcgatc agcgctcagg gtgtgcagct
    79201 gatcaggcgt ctggatgtgc tgttgtagga acggagcttt ccgttcctct tcaatgctgc
    79261 gttctgccgg caggcaacgc ttgcgcaggc tgcatggcac tgcgcagatg ctgctcgaac
    79321 agctcgacaa tcgccggcca gccctgacgc gaggcatgta ggcgggcatt caggcgcacc
    79381 cggcgcaggc cctcgtggtc ggtcagcagc cagctggcgg cgtcgataaa gccttgctca
    79441 tcttcggcca ccgccagcgc gccgttatgc ccgtggcgga tatgttgcgc ggccgccgcc
    79501 tgatcaaacg ccaccacacc aagcccggag gccagggctt cgagcaccac attgccgaag
    79561 gtttccgaca ggctgtgaaa cacaaacaga tcgcccgagg cgtaatgcgc cgccagctct
    79621 tcgccgcgtt gcaggccgca gaacatcgca tcaggcagtt gctgctgcag gctggcgcgc
    79681 tgcggaccgt cgccgaccag aatcagtttc agccgccgtt gcgggtgcgc ctgttgcagc
    79741 gcctgaaagc tcttgctgag cagggccagg ttcttttctg cggccaggcg cccgacatgc
    79801 aggatggcga tgtcctgttc cgccaggccc cagcgctcgc gcaaagctgt gcagcgtttg
    79861 gtcgggtgga acagctggct gtcgacgccc cgcgagagca gttccagatt gtcgaaacca
    79921 cgccgctgca gttcgatctg ctgactgaca ctgggcacca gggtcatctg cgagcggtta
    79981 tggaaccagc gcaggtaatt ggtcagggcg cgggtcagca ggccgacacc gtaatgcccg
    80041 gtgtactgct ggaagttggt gtgaaaaccg ctgaccaccg ggatctgcaa gcgccgcgcg
    80101 gcgcgcaggg cggacagtcc cagcggccct tcggtggcga tatacagcac atccgggcgc
    80161 tgccgttgcc agttgcgcag cagcttgtgc atcgacgact gaccccactg caaaccgggg
    80221 tagccgggca gcggccagcc acgggttagc gataaatcct gatcggcagc gccgcgcggg
    80281 tcgacgctct gtcgcggacg caccagttgc acgcgatggc cacggccgcg caggccatcg
    80341 accaggcgac cgagggtgtt ggccacgccg ttgatttccg gcgggaaggt ttcgctgatc
    80401 agcgcgatat gcagggaagc tgtgctcatg gcagcagtgt ccgctgccct tatgtagtga
    80461 ctgtgacgct ttgattgcgt ttgtatttca gtcgagcagc tcaatcagtc gctggcgcag
    80521 ccaggcgtgt gccgggtcga ggtgctgctg gtggtgccag atcaggctga cctgcaccgg
    80581 cggcatatcg aagggcagcg gcgcgatttc cagcgcatgc actggggcga acgcttgggc
    80641 caggcgcgca ggcacggtgc cgagcagatc ggtctgagca acgatggctg ggattggcag
    80701 gtagttgggc aaatgcaacg ccgcctgtcg tcgcaccctg gccgagccga ggacgatttc
    80761 cagcggcgaa ccacgcccgg ctcgcggcgt aaccgtgata tgccgcgcct gttggtaatc
    80821 ctccaggctc agcccgtcgg aaaaggccgg gtggccggcg cggccgatgg ccaccagcgg
    80881 ttcttcgcgc agcggcgtgt agtgcaggtc gggcgaatcg aagtgcagat agtcgatggc
    80941 caggtccagc gcgccgcttt ccagacgctg taccaggctg tcggcatcat cggcttgcac
    81001 cagcagctcg acctgcgcgg cattgcgcgc caaatgtgcc tgcaacatgg gcagcagcgc
    81061 ggcttgcgcg tagtcgttaa gcgacaggct gaaggcatgc ggcgtttgtg gatcaaacgg
    81121 ctcggccggg cccaggccgg tctgcagcag gcgcagggcc tgacgcactg gtacatagag
    81181 ggcctgggcg cgggcggtgg gggtcatgcc gcgggcggtg cgctggaaca gctcttcacc
    81241 cagttgctgg cgcaggcggg cgagggcatt gctcaccgtc gactggctta ggtgcaggcg
    81301 ctcggcggcg cgggtcaggt tgccttcctg catcagcgca tcgaagacca gcagcagatt
    81361 gaggtcgagc tgacacaaat tcgttatcaa gaataatgct ctttttaaat atccattatt
    81421 cgaatgatct ggccgcgcct agactgctgt caatccgcac aggagtaatg ccgtggccta
    81481 taacaacgac aatgcccaga ctttccgcac acgttatcgg gatgccatcc acccgctgta
    81541 caacccctgg ctgcatgccg tctttgtgct ggcttacggc ctgacctgca ttagtctgct
    81601 gtggcgcacg ctggacgcgg tggcgctctg gcaatggctg ctggtgccgc tgagcctggt
    81661 gttcttcagc tggggcgagt accaggtgca caagcgcctg gggcacaaca agacgcgctt
    81721 cggtcaactg ttctacaagc gccacacggg cgatcaccac agcttttttg tcgaaggtca
    81781 gatgcgctac gagacgccgc gtgactggcg ggtgatcctc tttcctgcct ggttgatcgt
    81841 gctctacagc ctgccgctgc tgggtatctg gtggctgctg gcgc
//
LOCUS       DASRUM010000040         6189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1025711, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000040 DASRUM010000000
VERSION     DASRUM010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025711"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1844
                     /locus_tag="VFF22_03275"
                     /pseudo
     CDS             1..1844
                     /locus_tag="VFF22_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286048.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1853..2428
                     /locus_tag="VFF22_03280"
     CDS             1853..2428
                     /locus_tag="VFF22_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZX15874.1"
                     /translation="MPTLEWLMTWQEPLLRTGQIILIVLLAWLFQRILTRGITRLGKR
                     YPQLPAELLMPLRGLLRWLIVGSAFMLVLERLGVSAQVLWTALTGFAAVAAVAFFAIW
                     SVLSNMFCALLIFAMGPFRIGECVEVLESADKPGVRGRVVAINLFYTTLEDLSGDAPG
                     AWVQIPNSLFFQKALRRWRSGDIPPVRTSES"
     gene            2434..3201
                     /locus_tag="VFF22_03285"
     CDS             2434..3201
                     /locus_tag="VFF22_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HZX15875.1"
                     /translation="MEWLASPELWVAFLTLTALEIVLGIDNIIFISILVSRLPKHQQP
                     KARFFGLALAMGTRILLLLSISWVMRLTADLFHVFDQGFSGRDLILLFGGLFLLWKST
                     AEIFHSLEGEEEVQAAGGKAYGFMGIIVQIAIIDIVFSLDSVITAVGLVDNVPVMVAA
                     IVISVIVMMLSAGTISDFIDKHPSLKILALSFLIVVGTVLVAEAFGVHVPKGYVYFAM
                     AFSLAVEAVNIRMRVARARARKEAEPESVQLRKDLPS"
     gene            3288..3917
                     /gene="rhtB"
                     /locus_tag="VFF22_03290"
     CDS             3288..3917
                     /gene="rhtB"
                     /locus_tag="VFF22_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HZX15876.1"
                     /translation="MALETWLAFFVACWVISLSPGAGAIASMSAGLQYGFLRGYWNAL
                     GLQLGLALQIAIVAAGVGAILAASELAFSLIKWFGVVYLVYLGYRQWQALPSDMTAQT
                     ERPIGRPLTLVLRGFLVNFSNPKAIVFMLAVLPQFLNPQAPLLMQYLVMGVTMICVDL
                     IVMAGYTGLAARVLGALRSPRQQKLMNRTFGALFVGAAALLASVRRAPV"
     gene            complement(3933..4706)
                     /locus_tag="VFF22_03295"
     CDS             complement(3933..4706)
                     /locus_tag="VFF22_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZX15877.1"
                     /translation="MSFWQRLEEPAHVVVCGASRGIGLALTTQLLARDDVAGVWAIAR
                     QASQAPGLMEMTAQYGQRLHYLDVDVCDEQALSELAAQLRAAIPRLHLLLCTVGVLQD
                     GAAKAEKGLAQINLVGLLATFQRNSFAPILLLKHLLPLLRGQHACTVAALSARVGSIG
                     DNRLGGWYSYRASKAALNQLLHTASIELSRLNPNSCVLSLHPGTTDTELSKPFQANVP
                     ADKLFTPSYSAECLLAQIEQHGPAQTGTFWGWDGQPIPW"
     gene            4828..>6189
                     /locus_tag="VFF22_03300"
     CDS             4828..>6189
                     /locus_tag="VFF22_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010443864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZX15878.1"
                     /translation="MTSVLRFCSWLLVPVLALCSLAVMAEPIEGAAQALHLLGYLGAD
                     YPATVANGQVIDSGEYREQLEFLTVLQGLIVALPARNEQAELQQGVASLRQGIEQRVE
                     GSQVAQQARQLSATLATAYGVSQTPAITPDPSRGAPLFAQHCSVCHGDSGAGDGPAGI
                     GLEPPPANLRDLARLNRLSLYDLFNTIGLGIDGTDMAAFADQLDERQRWDLASYIAGF
                     SAEPVAAGEPFSLSALAGMTPDEVAAAQGEEAAARFRAQRAQPPAQQRGPLQLIEHTR
                     ATLEQSLAAYRAGDHEQAYDLSVGAYLEGFELVESALDNLDSVQRKTTERALMGYRQA
                     LQDGLSVPQAAQRLEEAKAELATSTALLDSDGLSDSLSFFSSLLILLREGLEAILVLA
                     AILAFLRNTGQQQAVRSVHIGWGLALLAGAGTWALAAYLIDVSGAQRELLEGVTALFA
                     SVMV"
BASE COUNT         1046 a         1897 c         2017 g         1229 t
ORIGIN      
        1 ccctgcaccc cggccacaaa gccggcctga tcggcgctaa cggcgccggc aaatccagcc
       61 tgtttgccct gtttcgtggc gagctgagtg cggacgcggg cgactgcctg atcccagtcg
      121 attggcgcgt cgcgcatatg cgccaggaga tcgatgcggt cgagcgccag gcggtcgatt
      181 atgtactcga tggcgacctg caactgcgca aggtgcaggc cgaattggtc gtggccgaag
      241 ccgcgcatga cggcgccgca gtggcgcgtt tgcacaccga gctcgatacc ctcgacggct
      301 acagcgccga tgcccgtgcg cgcaaactgc tcgccggcct gggtttcgac agtgcgcaga
      361 tggaccgtcg cgtcggcgac ttctccggtg gctggcgtat gcgcctgaac ctggcgcagg
      421 cgctgatgtg cccgtccgat ctgctgctgc tcgacgagcc gaccaaccac ctcgatctcg
      481 acgcgattct ctggctggaa agctggctga aaggttaccc cggtaccttg ctgctgatct
      541 cccatgaccg cgatttcctg gatgccgtag tggaccacgt tgcccacctc gatcagcaga
      601 aactgaccct ctatcgcggc ggctattcgg ccttcgagcg cacccgtgcc gagcgactgg
      661 cgcagcagca acaggcgtac gagaagcagc aggtgcagcg cgcgcacatg gaaaagtaca
      721 tcgcccgctt caaggcccag gccaccaagg cgcgccaggc gcagagccgg atcaaggcgc
      781 ttgagcgcat ggaagagctg tcggcggcgc atgtcgattc gccgtttgac ttcagcttcc
      841 gtgaggccga caagatttct agcccgctgc tcagtctgag cgaaggccgc ctcggttatg
      901 gcgacaaggt ggtgctggag aaggtcaaac tgaacctcgc gcccggcgcg cgcctgggcc
      961 tgctcgggcc gaacggtgcg ggtaagtcga ccctgatcaa gaacctgtcg aacgagctgc
     1021 agcccatcag cggccatctg cagcgcggcg agaatctggt ggtcggctac tttgcccagc
     1081 atcagctcga tgccctggat gacaaggcca gcccgctgct gcacttccag cgtctggcgc
     1141 cgaccgagcg cgagcagacc ctgcgtgact tcctcggcgg cttcgacttc cgcggcccac
     1201 gttgcgacta gccggtgctg aatttctccg gcggtgagaa agcccgtctg gccttggcgc
     1261 tgattgcctg gggcaagcct aacctgctgc tgctggatga gccgaccaac cacctcgacc
     1321 tggaaatgcg cctggcgctg accatggcgc tgcaggaatt tagcggcgca gtggtggtgg
     1381 tgtcgcacga tcgccatatg ctgaaaagca ccactgacga gttcctgctg gtcgccgatg
     1441 gtcaggtgct gccgttcgac ggtgatctgg aagactacgc ccgctggctg gtggattacc
     1501 gcacgcggca ggcgccgacg agcggtggcg agagcaatcc ggataaagcc gacaagcgcg
     1561 ttcagcgcca ggccgcagcg gccttgcgtc agcagttggc gccgcacaag cgcgaagccg
     1621 acaagctgga gaaagagctg gggcagttgc atgagaagct cgccgccgtt gaagcgcgtc
     1681 tgggtgacag cgacatctac gaagccgcgc gcaaggatga gctgcgcgac gccctggccg
     1741 agcgggccaa gctgaaaagc cgcgaagccg atcttgagga gcgctggatg ctcgcgctgg
     1801 aaaccctcga atccctgcaa gccgagctgg aggccgccag ttgaatatcg ccctgccgac
     1861 cctggaatgg ctgatgacct ggcaagagcc gttgctgcgc accggtcaga tcatcctgat
     1921 tgtgctgctg gcctggctgt tccagcgcat cctcacccgc ggcatcaccc gactcggcaa
     1981 acgctacccg cagctgccgg ccgaattgct gatgcccctg cgcggtctgc tgcgttggtt
     2041 gattgtgggt agcgcattca tgctcgtgct ggaacgcctg ggggtgtcgg cacaggtgct
     2101 gtggaccgcg cttaccggct ttgcggcggt ggctgcagtc gcgttcttcg cgatctggag
     2161 cgtgttgtcc aacatgttct gtgcgctgct gatcttcgcc atggggccat ttcgcatcgg
     2221 cgaatgcgtc gaagtgctgg aaagcgctga caaaccagga gtgcgcggcc gggtggtggc
     2281 gatcaatctg ttctacacaa ccctggaaga tctcagcggt gatgcacctg gcgcctgggt
     2341 gcagatcccc aatagcctat tttttcaaaa agccttgcgc cgctggcgca gtggcgacat
     2401 cccacctgta cgtactagcg agagttaatc cctatggaat ggctggccag ccctgaactc
     2461 tgggtcgcat ttctgaccct gaccgccctg gaaattgtcc tgggcatcga caacatcatc
     2521 tttatctcga tcctggtcag tcgtctgccc aagcatcagc aacccaaggc gcgcttcttc
     2581 ggcttggcct tggcgatggg tacgcggatt ctgctgctgc tgtcgatcag ctgggtgatg
     2641 cgcctgacgg ccgacctgtt ccatgtgttc gatcaaggct tctccgggcg tgacctgatc
     2701 ctgctcttcg gtggcctgtt cctgttgtgg aagagcactg ccgagatctt ccacagcctg
     2761 gaaggcgaag aagaagttca ggctgccggc ggcaaggcct acggctttat gggcatcatc
     2821 gtgcagatcg cgatcatcga catcgtcttc tccctcgact cggtaatcac tgcggtcggc
     2881 ctggtcgaca acgtgccggt catggtcgcg gccatcgtca tctcggtgat cgtgatgatg
     2941 ctgtcggcag gcaccatcag cgactttatc gacaagcacc cgagcctgaa aatcctcgcc
     3001 ctgtcgttcc tgatcgtggt cggtactgtg ctggttgccg aagccttcgg cgtgcatgtg
     3061 ccgaaggggt atgtgtactt cgccatggcc ttctctctgg ccgtggaagc ggtcaatatc
     3121 cgcatgcgtg tggcccgcgc acgggcccgc aaggaagcag agccggagtc ggtgcaactg
     3181 cgtaaagact tgccgagctg atcgacaggc tgggcgcggt aactgacaag gggtaggcta
     3241 gatggcctac ccctttttat tccgctgtct gttaatggag aagtgccatg gcactggaaa
     3301 cctggcttgc gttctttgtg gcctgttggg tcatcagcct gtcgccgggc gccggggcga
     3361 ttgcctcgat gtccgccggc ttgcaatacg gctttctgcg cggctactgg aatgccctgg
     3421 gcctgcagct gggcctggct ttgcagattg ccatcgtggc cgccggtgtc ggggcgattc
     3481 tggcggcatc ggagctggcc ttcagcctga tcaaatggtt tggcgtggtc tatctggtgt
     3541 acctcggcta tcgccagtgg caggcgctgc ccagtgatat gacggcgcag accgagcgcc
     3601 ccatcggccg gccgctgacc ttggtgttgc gcggttttct ggtcaacttc agtaacccca
     3661 aggcgattgt ctttatgctc gcggtgctgc cgcagttcct caatccacag gcgccgctgc
     3721 tgatgcagta cctggtgatg ggcgtgacca tgatctgcgt cgacctgatc gttatggccg
     3781 gctacaccgg cctggctgca cgggtgctgg gcgcgctgcg ttcgccacgt cagcagaagc
     3841 tgatgaaccg caccttcggc gcgctgtttg tcggtgcggc ggcattgctg gccagcgtgc
     3901 gccgcgcgcc ggtctgaact gcgcgggttg agttaccagg gaatcggctg gccgtcccag
     3961 ccccagaacg tgccagtctg cgccgggcca tgctgttcga tctgcgccag caggcactcg
     4021 gctgaatagc tcggggtaaa cagcttgtcg gccggcacat tggcctggaa gggtttcgac
     4081 agttcggtgt cggtcgtacc aggatgcagg ctgagcacgc agctgttggg gttaaggcgg
     4141 ctcaactcga tgcttgcagt gtgcagcaac tggttcagtg cggctttgct ggcgcggtag
     4201 ctgtaccagc cgccgaggcg attgtcgcca atcgaaccga cccgcgccga cagcgccgcc
     4261 accgtgcagg catgctgacc gcgtagcagc ggcaacaggt gcttgagcag caggatcggt
     4321 gcaaagctgt tgcgctgaaa ggtcgccagc agccccacaa gattgatctg cgccaaaccc
     4381 ttctcggcct ttgccgcgcc gtcctgcagc acgccaaccg tgcacagcag cagatgtaga
     4441 cgcgggatgg cagcgcgcaa ttgcgcggcc agctcgctca gcgcttgttc atcgcataca
     4501 tccacatcca ggtagtgcag gcgttggcca tattgcgctg tcatctccat caggccgggc
     4561 gcctgactgg cctggcgggc gatggcccag accccggcga catcgtcgcg ggcgagcaat
     4621 tgtgtggtca gcgccaggcc aattccccgg ctggcgccac agaccacaac atgcgctggt
     4681 tcttccaggc gttgccaaaa actcatcgca tcactcctgc gccgttgcga cgatccgtcg
     4741 cgggccatgg tcgatcagtg gttgaggaaa gtcgttagga gggtaacaat ctaatgtcat
     4801 ccattcgtat ttgggatcaa gtcctatatg acttccgtat tgcgtttctg cagttggttg
     4861 ctggtgccgg tgttggcact ctgcagcctg gctgttatgg ccgagcccat cgagggcgcg
     4921 gcgcaagctc tgcatctgct cggttacctg ggcgctgact atcctgccac cgtggccaat
     4981 ggtcaggtga tcgactccgg cgaataccgc gagcagctgg agtttcttac tgtactgcaa
     5041 ggtctgatcg ttgcgctgcc agcgcgtaac gaacaggccg agttgcaaca gggcgtggcc
     5101 agcctgcgcc agggcattga acagcgtgtc gagggcagcc aggtcgctca gcaggcgcgg
     5161 cagttgtccg cgaccctggc tacggcctat ggcgtcagcc agaccccggc gattacgccc
     5221 gaccccagcc gtggcgcgcc gttgttcgcc cagcattgca gcgtctgcca tggcgacagc
     5281 ggcgcgggtg acgggccggc gggcatcggc ctggaaccgc cgccggccaa cctgcgcgat
     5341 ctggcgcggc tcaaccggtt gagcctttat gacctgttca acaccatcgg cctgggtatc
     5401 gatggcaccg atatggcggc ctttgccgat cagctggatg agcgccagcg ctgggatttg
     5461 gccagttata tcgccggctt cagcgccgag ccggttgccg caggcgagcc gttcagcttg
     5521 tcggcgctgg ccggtatgac gccggatgaa gtggcagcag cacagggcga ggaggcggct
     5581 gcgcgtttcc gtgcccagcg tgcgcagccg ccagcgcagc aacgcggccc gctgcaattg
     5641 atcgaacaca cccgcgcgac cctggagcag agcctggcgg cctaccgcgc cggtgatcac
     5701 gagcaggcct atgacctctc cgtcggtgcc tatctggaag gcttcgagct ggtcgaaagc
     5761 gctctggaca accttgactc ggtgcagcgc aagaccaccg agcgagcgct gatgggctac
     5821 cgccaggcgc tgcaggatgg cctgtcggtg ccgcaggcgg cacaacggct ggaggaggcc
     5881 aaggccgagc tggcgacttc caccgcgttg ctcgacagcg atggtctgtc ggattcgctg
     5941 agtttcttct ccagcctgct gattctgctg cgtgaagggc tggaggcgat tctggtgctg
     6001 gcggcgattc tcgctttcct gcgcaacacc ggtcagcaac aggcggtgcg cagcgtgcat
     6061 atcggctggg gtctggcgct gctggccggc gcgggcacct gggcgttggc ggcgtatttg
     6121 attgatgtca gcggcgcgca gcgtgaactg ctcgaaggcg tgactgcgct gtttgccagc
     6181 gtgatggtg
//
LOCUS       DASRUM010000041        10993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2192076, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000041 DASRUM010000000
VERSION     DASRUM010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 10993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10993
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2192076"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            389..835
                     /locus_tag="VFF22_03305"
     CDS             389..835
                     /locus_tag="VFF22_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX15879.1"
                     /translation="MHIQAATAVDLDELAELFGAYLRFYEVAKPQAQVRDFLAARLRN
                     NDSIILLARDAQGAALGFVQLYAFFSSLALQPAWLLSDLYVSEQARRQGVGEALMNAA
                     RAHAEATGACGLQLETAKSNLPGQALYERLGYVRDEVFFTYWLALK"
     gene            858..1376
                     /locus_tag="VFF22_03310"
     CDS             858..1376
                     /locus_tag="VFF22_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZX15880.1"
                     /translation="MDHLVLTVIAQDQPGLVERIAQCISEHGGNWLESRMSRMAGQFA
                     GILRVDVPLDAHSGLVTALEALQAQGIRVQIAAGGTEPVTVWQAIRLELVGNDRPGIV
                     RDITRLLSSHGVNLESLNTEVVPAPMSGELLFQAQAVLGVPQSLSLEQLQTHLETLAD
                     DLMVELKLQAEE"
     gene            complement(1783..2676)
                     /gene="rarD"
                     /locus_tag="VFF22_03315"
     CDS             complement(1783..2676)
                     /gene="rarD"
                     /locus_tag="VFF22_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019437061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HZX15881.1"
                     /translation="MATANPRRGYILGLTAYIIWGLFPLYFKAIQSVPALEIIVHRAL
                     WSALFGALLLLVWKHPGWLRELLNNPKRFAVLALSCVLIASNWLVYVWAVNNERMLEA
                     SLGYYINPLVNVLLGMLILGERLRRLQWLAVALAALGVLQQLWQVGSIPWVSLVLALT
                     FGFYGLIRKQAPVAALPGLVVETWLLLPLALGWLILHPVAMSTQADFWSTPEALWLSA
                     AGPITLVPLVCFNAAARHLPYTTLGFLQYLAPTLVLLQAIFLFDEHFDPSKLLAFICI
                     WAGLAIYSVDAWLSLRKRQAV"
     gene            complement(3006..3980)
                     /locus_tag="VFF22_03320"
     CDS             complement(3006..3980)
                     /locus_tag="VFF22_03320"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HZX15882.1"
                     /translation="MSHPFAALTPDLVLDAVESIGYLSDARVLALNSYENRVYQVGIE
                     DETPLIAKFYRPDRWTDEAIREEHSFTFELAECEVPVVAPLVRDGETLFSYQGFRFAL
                     FPRRCGRAPEPGNLDQLYRLGQLLGRLHAVGSLKPFAHREALQVSNFGHASLATLLDG
                     NFIPRSLLPAYESVARDLLKRLDTLFAAHPVQAIRLHGDCHPGNLLCRDDTFHMVDLD
                     DCRMGPAVQDLWMMLAGERHERLGQLSELMDGYQEFHDFNPRELPLIEGLRTLRLMHY
                     SAWLARRWDDPAFPMSFPWFGSERYWGDQVLILREQLSALDEEPLKLF"
     gene            complement(4035..4769)
                     /locus_tag="VFF22_03325"
     CDS             complement(4035..4769)
                     /locus_tag="VFF22_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007966532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HZX15883.1"
                     /translation="MRCQPNSQHQVYNWLKRKQSCLLCDEAADAALAICTSCEAELPW
                     LGGHCLVCALPLPSQGLVCGACLKKPPSFSKVEVPWRYAFPIDSLITRFKHQAKWPLG
                     RLLGELLSHHLLHAFDEGLSKPDLLLPVPLANKRQRQRGFNQAGLLCEWLSPLLQVPL
                     QAHWLQRVIDTPAQQQLDAATRKRNLRKAFALTPGSAVSGLHVALVDDVLTTGATAES
                     LARLLKKAGAARVDVYCLARTPKPGD"
     gene            4850..5905
                     /gene="bioB"
                     /locus_tag="VFF22_03330"
     CDS             4850..5905
                     /gene="bioB"
                     /locus_tag="VFF22_03330"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017138024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HZX15884.1"
                     /translation="MSATTAANLRHDWTLNEVKALFEQPFNDLLFQAQTVHRVHFDQN
                     RVQVSTLLSIKTGACPEDCKYCPQSGHYNTGLEKEKLLEVQKVLEAAAEAKAIGSTRF
                     CMGAAWKHPSAKDMPYVLKMVEGVKALGLETCMTLGKLDKEQTQALAAAGLDYYNHNL
                     DTSPEFYGNIITTRTYGERLETLAYVRDSGMKICSGGILGMGESLDDRAGLLIQLANL
                     PEHPESVPINMLVKVKGTPLADEQDVDPFDFIRMLAVARIMMPQSHVRLSAGREAMNE
                     QMQALAFFAGANSIFYGEKLLTTANPQANQDMQLFARLGIKPEEREEHADEVHQAAIE
                     QALVEQRDSKLFYNAAV"
     gene            6013..7194
                     /gene="bioF"
                     /locus_tag="VFF22_03335"
     CDS             6013..7194
                     /gene="bioF"
                     /locus_tag="VFF22_03335"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008054547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HZX15885.1"
                     /translation="MSFDLSSRLAERRAANLYRQRPLLESPQGPLVMVDGRELLAFCS
                     NDYLGLANHPKVVEAWRAGAEKWGVGGGASHLVIGHSTPHHQLEEALAEFTGRPRALL
                     FSTGYMANLGAVTALVGQGDSVLEDRLNHASLLDAGLLSGARFSRYLHNDGVSLANRL
                     RKVIGNTLIVTDGVFSMDGDLADLPSLCAEARRANAWVMVDDAHGFGPLGATGGGIVE
                     HFGLGLDDVQVLVGTLGKAFGTAGAFVAGSDDLIEALVQFARPYIYTTSQPPALACAT
                     LKSLELLRSEHWRREHLNALIKRFREGAQAIGLELMDSATPIQPILIGDSGRAMQLSQ
                     MLRERGLLVTAIRPPTVPAGSARLRVTLSAAHSLEQLELLLEALAECWPLLTAEGAGH
                     A"
     gene            7187..7918
                     /locus_tag="VFF22_03340"
     CDS             7187..7918
                     /locus_tag="VFF22_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZX15886.1"
                     /translation="MRDRLILLPGWGLGTAPLQPLADALRGLDPRLHVQIEPLPLLDS
                     SDPADWLDELDANLPQDAWLGGWSLGGMLAAELAARRGERCSGLLTLASNLRFVTEPS
                     WPAAMPADTFNAFRQGCAADASTTLKRFALLCAQGAVDARGLSRQLLGAAPQPQSAIL
                     LVGLDLLASLDTRSALQAFVGPQLHLFAGADALVPAGAAPALLELLPDVEIGLINNAS
                     HAFALERPHEVAAAIQAFLHEAGDD"
     gene            7982..8791
                     /gene="bioC"
                     /locus_tag="VFF22_03345"
     CDS             7982..8791
                     /gene="bioC"
                     /locus_tag="VFF22_03345"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102130 - malonyl-CoA methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HZX15887.1"
                     /translation="MSEAFQPGGLPDKRQVAASFSRAAESYDSVAALQRDVGRQLLAQ
                     LPASISPQRWLDLGSGTGYFSRALGERFPASQGMALDIAEGMLQHARPLGGAQQFIAG
                     DAERLPLRDASCELLFSSLALQWCADFSAVLSEAERVLKPGGVLAFSSLCVGTLQELS
                     DSWHAVDGFVHVNRFRRFEDYQRLCDASGLARLSLQTTPETLYYPDVRSLTHELKALG
                     AHNLNPGRPGGLTGRARMLALLNAYERFRQPRGLPATYQVVYGVLRKETTR"
     gene            8788..9477
                     /gene="bioD"
                     /locus_tag="VFF22_03350"
     CDS             8788..9477
                     /gene="bioD"
                     /locus_tag="VFF22_03350"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HZX15888.1"
                     /translation="MSAAFFVTGTDTEIGKTTIAAGLLHAARLAGLSTAAAKPVASGC
                     VRTADGLRNDDALALLGECTLALRYEEVNPLAFEPAIAPHLAAREAGVLLDVAALQGP
                     VQAILDKRADLTLVEGAGGWRVPLAGQAALSDLAIALQLPVILVVGVRLGCINQAVLT
                     AEAILRDGLPLAGWVANLIDPQTSCLEDNLATLAERLPAPCLGYVPRLAEATPAAVSG
                     YLSFGLLHRAL"
     gene            9572..9871
                     /locus_tag="VFF22_03355"
     CDS             9572..9871
                     /locus_tag="VFF22_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15889.1"
                     /translation="MEISGSAFNSGISTIQSGQRRVDQAAADIASNALGPQPQPTSAP
                     PANQVNPAPSSAEQTRPDLAESLVALRQGQNEAEAGAKVVKTADEVLGTLIDTTA"
     gene            10027..10716
                     /locus_tag="VFF22_03360"
     CDS             10027..10716
                     /locus_tag="VFF22_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metalloprotease CJM1_0395 family
                     protein"
                     /protein_id="HZX15890.1"
                     /translation="MSPDITRAAQAPITAAQPLADEQSASAREEADKPADSSANPDKA
                     KSREGEPSPQQQRLEQLEIAKLVSRDQEVRTHEQAHAAVGGRYAGAPSYTYERGPDGK
                     RYAVGGEVGIDTSPIPSDPEATLRKMEVVIRAALAPAEPSAQDRQVAAQAQAQMVEAR
                     AQLAQQQSSEAQAASEARAERRKEDEEESAEEDKSVAPARQNLPPPPSLDLYKQLSGL
                     QAPAPVVDLVA"
     gene            10882..>10993
                     /locus_tag="VFF22_03365"
     CDS             10882..>10993
                     /locus_tag="VFF22_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase N-terminal
                     domain-containing protein"
                     /protein_id="HZX15891.1"
                     /translation="MPDYKAPLRDIRFVRDELLGYEAHYQSLPGCQDATPD"
BASE COUNT         2044 a         3303 c         3485 g         2161 t
ORIGIN      
        1 atgccggtac cggggatggc ttcgttattc acgaagtcga acagcacttt ggcaacctgc
       61 aggctgccta cttgaacgcg ttcagtcatc acttgcctca ctctgctcag ctctactcaa
      121 tagccgcgtc ggacatggga aatacgtctt gtagtccgag gcgcggcata ctacatcaag
      181 atccggggaa aatcagtggg tgcgcgtcgc tctgcgacca agaagtgctt tgcagtcact
      241 gtgagtgacg tattgtcgcg gtaagatttg aatattgttt cataaaaaat aaaaaacagt
      301 acacagttta ttgcgtgttt ttctcgatct actgctatag gtgcgtctgc ctatactgct
      361 gctccaccgg ttattgagtg tgattaccat gcatatacag gcagccactg ccgtggatct
      421 ggatgaactg gccgagctgt ttggcgcgta cctgcgcttc tacgaggtgg ccaaacctca
      481 ggcgcaggtg cgggattttc tcgccgcgcg cctgcgcaac aatgactcga tcattctgct
      541 ggcccgtgat gcacaaggtg cggcgctggg ctttgttcag ctgtatgcat ttttttcctc
      601 gctggcgctt cagccggcct ggctgctgag tgacctgtat gtcagcgaac aggcgcgccg
      661 ccaaggcgtg ggcgaggcgc tgatgaacgc cgctcgcgcc catgccgagg ctactggtgc
      721 gtgcggtttg cagttggaga cggcaaagag caatctgcca ggacaagccc tctacgaacg
      781 cctgggttat gtgcgtgatg aggtgttttt tacctattgg ctagcgctca aataagactc
      841 gtattaagga gttgttcatg gaccatctgg tattgacggt tattgcccag gatcagccgg
      901 ggctggtcga gcgcatcgcc cagtgcatca gcgagcacgg tggtaactgg ctggaaagcc
      961 gaatgtcgcg catggccggg cagttcgccg gaatcctgcg ggtcgatgtg ccgctggatg
     1021 cgcactcggg cctggttacc gcgctggaag ccttacaggc gcagggcatc cgcgtgcaga
     1081 tcgccgctgg cggcaccgaa ccggtaacgg tttggcaggc cattcgcctg gagctggtgg
     1141 gcaatgatcg cccaggcatt gtgcgcgata tcacccgcct gctcagcagc cacggggtca
     1201 acttggaaag cctgaacacc gaggtggtgc cggcgcccat gagtggcgag ctgctgttcc
     1261 aggcccaggc tgtgctgggc gtgccgcaga gcctgagcct agagcagttg cagacgcacc
     1321 tggaaaccct ggctgatgat ctgatggtgg agctaaagct gcaggctgaa gagtaacgtc
     1381 gagggttatc cacgatcgat gtggataact ctgtgcgtag catgaggagc cccggagcac
     1441 tgcctagtgc cccggggctt tttgctaagt gcttattttt taagcactta gaagctggtt
     1501 gtgcacaaag gccggggatg cttctgtgga taagccgggg gcaactggct gcaggccagg
     1561 tgcagtgccc gctgcatggg attggttgtt ttttaggcga tgtcctgatg ccgtgttgct
     1621 cttggttcgc cgctaagggt agatggccgg gctttgtagg gtgtgccgtg cgcaccaaga
     1681 atctgccgct gaagttgagg ccagagcctg agccagagtt ggtgcgcacg gcgcacccta
     1741 cctatatggc ccctgatccc gccatacagc attcagccgc ttttataccg cctggcgttt
     1801 gcgcaggctc agccaggcat ccacgctgta gatcgccaaa cccgcccaga tgcagataaa
     1861 ggccagcagt ttgcttgggt cgaaatgctc gtcaaacaga aagatcgcct gcaacagcac
     1921 cagggtcggc gccaggtact gcagaaagcc cagggtggtg tagggcagat ggcgggcggc
     1981 ggcgttgaaa catactagcg gcaccagggt gatcggcccg gcggcgctca gccagagcgc
     2041 ttccggggtg ctccagaaat cagcctgggt gctcatcgcg accgggtgca ggattaacca
     2101 gccaagcgcc agcggcagca gcagccaggt ttccacgacc aggccgggca gcgcggcaac
     2161 cggcgcctgt ttgcggatca gcccataaaa gccaaaggtc agcgccagca ccagcgacac
     2221 ccacggaatg ctgcccacct gccacaactg ctgcagcacg cccagcgcgg ccagggccac
     2281 ggccagccac tgcaggcggc gcaaacgctc gccgagaatc agcatgccca gcagcacatt
     2341 gaccaacgga ttgatgtaat agcccaggct ggcctcaagc atgcgttcgt tattcaccgc
     2401 ccacacatac accagccagt tgctggcgat cagcacacag ctcaaggcca gtacggcaaa
     2461 gcgtttgggg ttattcagca gttcgcgcag ccagccggga tgcttccaca ccagcagcaa
     2521 cagcgcgccg aacagcgcgg accagagtgc gcgatgaacg atgatctcca gcgccggcac
     2581 gctctggatc gctttgaaat agagcgggaa caagccccag ataatgtagg cggtcaggcc
     2641 gagaatatac ccgcggcgcg ggttagcggt ggccatgcag ggtccttgct taggctgcta
     2701 acgagaggct tgcgattcta gtcggcatgg tgcgggcttg tctggatggg caggattttc
     2761 ttcggcgccc taggccactt ggctgtaaca aagacgttac ggctacaggg ctaactgtag
     2821 tggtcagtcc tgtagcccgg ataagccttg gcgcaatccg ggagtgctat cccggattgc
     2881 gctgcgctta tccaggctac ggactgcatg gccgagggtt cttggtgcgc gcggcgcacc
     2941 ctacaagtca gtcctgtagc ctggatgcaa tccgggggcg ccattcccgg attacataag
     3001 cagtgtcaaa acagcttgag cggctcttcg tccagcgccg acagctgctc acgcaggatc
     3061 aaaacctgat cgccccagta acgctcgctg ccgaaccagg ggaagctcat ggggaaagcc
     3121 gggtcgtccc agcgccgtgc cagccaggcg ctgtaatgca tcaggcgcag ggtgcgcagg
     3181 ccttcgatca gtggcagttc gcgcgggttg aagtcgtgaa actcctgata gccatccatc
     3241 agctccgaca gctggccgag gcgttcgtgg cgctcaccag cgagcatcat ccacaagtcc
     3301 tgcacggccg ggcccatacg gcagtcgtcg aggtcgacca tatggaaggt gtcgtcgcgg
     3361 cacagcaggt tgcccgggtg gcagtcgcca tgcaggcgaa tcgcctgcac cgggtgggcg
     3421 gcgaacagcg tatccaggcg cttgagcaga tcccgcgcca ccgactcgta ggccggtagc
     3481 aggctgcgcg gaataaagtt gccatcaagc agggtggcca gcgatgcatg gccgaagtta
     3541 ctcacctgca gggcttcacg gtgggcaaac ggcttgagtg agccgaccgc atgcaggcgg
     3601 ccgagcagtt ggccgagacg atacagttgg tcgaggttgc caggctccgg cgctcggccg
     3661 caacggcgcg gaaacagggc gaagcgaaag ccctggtaac tgaaaagggt ttcgccgtcg
     3721 cgtaccagtg gcgcaaccac cggtacttcg cactcggcca gctcgaaggt gaagctgtgc
     3781 tcttcgcgaa tcgcttcatc ggtccagcgg tctgggcgat agaacttggc gatcagcggc
     3841 gtttcgtcct cgatgcccac ctggtagacg cgattttcat agctgttcag cgccagtacg
     3901 cgggcgtcgc tgaggtagcc gatgctctcc acggcatcca gcaccaagtc gggtgtgagt
     3961 gcggcaaatg gatgggacat gggtgactcc agcaggtggg ccgcaaagtg taacccttaa
     4021 ccgccgttag ccgctcagtc gccaggtttc ggtgtgcgcg ccaggcagta cacatcaacc
     4081 cgcgctgcgc cggccttctt taagagccgc gccaggcttt cggcggtggc gccggtggtc
     4141 agcacatcat cgaccagcgc cacatgcagg ccgctgactg cactgcccgg cgtcagggca
     4201 aaggccttgc gcaggttgcg tttgcgcgtg gcggcgtcca gctgctgttg tgccggggtg
     4261 tcgataaccc gctgcagcca gtgtgcctgc aacggcactt gcagcaaggg acttaaccat
     4321 tcacagagca gaccggcctg attaaagccg cgctggcgtt ggcgtttgtt cgccagcggt
     4381 acgggcagca acaggtcggg tttgcttagc ccttcatcga aggcatgcag caggtggtgt
     4441 gacaaaagct caccgagcag acgtcccaat ggccacttgg cctgatgctt gaagcgggta
     4501 atcaggctgt cgatggggaa ggcgtagcgc cagggcactt caactttgct aaagctcggc
     4561 ggctttttca ggcaggcccc acataccagg ccttggctgg gtagcggcag ggcgcagacc
     4621 aggcaatgcc cgccgagcca gggcagctcg gcttcgcagc tggtgcagat ggccagtgcg
     4681 gcgtctgcgg cttcatcgca taacaagcag gattgtttgc gttttaacca gttgtaaacc
     4741 tggtgttgtg agttgggttg acagcgcata cgccttccat aactatgccg aacatccgtg
     4801 tcgcgtccgc tgaatctagc agggtgcagc caacaataag gaattaccga tgagcgcaac
     4861 caccgccgcc aacctgcgtc acgactggac tctgaatgag gtcaaggcgc tcttcgagca
     4921 accgttcaac gacctgctgt tccaggcgca gactgtgcac cgcgtgcact tcgaccagaa
     4981 ccgcgtgcag gtctcgaccc tgctatcgat caagaccggc gcctgcccgg aagactgcaa
     5041 atactgcccg cagtccggcc actacaacac cggtctggaa aaggaaaaac tgctggaagt
     5101 gcagaaagtg ctggaggccg ccgccgaagc caaggccatc ggttccaccc gtttctgcat
     5161 gggtgcggcg tggaagcatc cgtcggccaa ggacatgccc tacgtgctga agatggtcga
     5221 aggcgtcaaa gccctggggc tggaaacctg catgaccctg ggcaaactgg ataaagagca
     5281 gacccaggcc cttgccgctg ccggcctcga ttactacaac cacaacctgg atacctctcc
     5341 tgagttctac ggcaatatca tcaccacccg cacctatggc gagcgcttgg aaaccctggc
     5401 ctatgtacgt gactcgggga tgaagatctg ttccggcggc atcctcggta tgggcgagtc
     5461 gctggacgac cgcgccgggc tgctgattca actggccaac ctgccggagc atccggagtc
     5521 ggtgccgatc aatatgctgg tgaaagtcaa aggcacgccg ctggccgatg agcaggatgt
     5581 cgatcccttc gactttatcc gcatgctcgc cgtcgcgcgg atcatgatgc cgcagtcgca
     5641 cgtgcgcctg tccgccggcc gcgaggcgat gaatgagcag atgcaggccc tggcgttctt
     5701 cgctggcgcc aactcgattt tctacggtga aaaactgctg accaccgcca acccgcaagc
     5761 caatcaggac atgcagctgt tcgcccgcct cggcatcaag ccggaagagc gcgaagaaca
     5821 tgctgacgaa gtgcaccagg ccgccattga gcaggccctg gtcgaacagc gcgattccaa
     5881 gctgttctat aacgccgcgg tctaatccct tcgaaccggt aggagccagc ttgctgcggt
     5941 ccgacgtctc ggcgatttcg tttgccgctg atcgccagca agctcgctcc tacaaaagcc
     6001 aaccgggctg ccatgagctt cgatctatcc tctcgccttg ccgagcgccg cgccgccaat
     6061 ctctatcgcc aacgtcccct gcttgaatcg ccgcaggggc ctttggttat ggtcgatggc
     6121 cgtgagctgc tggccttctg ctccaacgat tacctgggcc tggccaatca tccaaaggtg
     6181 gttgaggcct ggcgcgccgg cgctgagaaa tggggcgtgg gtggcggtgc gtcgcatctg
     6241 gtgatcggcc acagtacccc acatcaccag ctcgaagaag cgctggccga gttcaccgga
     6301 cgcccccgcg cgctgctgtt ttccaccggc tatatggcca acctcggtgc ggtcaccgcg
     6361 ctggtcgggc agggcgatag cgtgctggag gatcggctta accacgcgtc cctgctggat
     6421 gccggcctgc tcagcggcgc gcgtttttcc cgttacctgc ataacgatgg cgtcagcctg
     6481 gccaatcgcc tgcgcaaggt catcggcaat accctgatcg tcaccgacgg cgtattcagc
     6541 atggacggcg atctggccga tctgccttcg ctgtgcgccg aagcgcgtcg cgccaatgct
     6601 tgggtgatgg tggatgacgc ccatggtttc ggcccgctgg gcgccaccgg cggcgggatt
     6661 gtcgagcact tcggcctggg cctggatgat gtgcaggtgc tggtcggtac cctgggcaaa
     6721 gccttcggta cggcaggtgc ctttgtcgcc ggcagtgacg atctgatcga agccctggtg
     6781 cagttcgcca ggccctatat ctacaccacc agccagccgc cggcgctggc ctgcgccacc
     6841 ctgaaaagcc tggagttgct gcgcagtgaa cattggcggc gcgagcacct gaatgccctg
     6901 atcaaacggt tccgcgaagg tgcccaggcc atcggcctgg agctgatgga cagtgccacg
     6961 ccgattcaac caatcctgat cggcgatagt ggccgtgcga tgcagctgtc gcagatgctg
     7021 cgcgagcgcg gtttgctggt cacggcaatt cgtccgccaa ccgtgccggc tggcagcgcc
     7081 cgcctgcgcg tgaccctgtc cgccgcccat agcctggaac agttggagct gctgctggaa
     7141 gccctggccg agtgctggcc gttgttgacg gctgagggcg caggccatgc gtgatcgact
     7201 gattctattg cccggctggg gcctcggcac tgcgccgttg cagcctctgg ccgatgcctt
     7261 gcgtggcctc gacccgcgtc tgcatgtgca gatcgaaccg ctgcctttat tggactccag
     7321 cgatccggcc gactggctgg atgagctgga cgccaatctt ccacaggacg cctggcttgg
     7381 tggctggtct ctgggcggca tgctcgccgc tgagctggcg gcgcgtcgtg gcgagcgttg
     7441 cagcggcttg ctgaccctgg ccagtaatct gcgttttgtt actgagccaa gctggccagc
     7501 ggctatgccg gccgacacgt ttaatgcttt ccgtcagggc tgcgccgccg atgcgtccac
     7561 taccctgaag cgttttgccc tgctatgcgc tcaaggcgct gtggatgccc gtggcctgtc
     7621 gcggcagttg ctcggtgcgg ctccgcaacc gcagtccgcc attctgttgg tcgggctgga
     7681 tctgcttgcc agcctggata cgcggtcggc gctgcaagcc tttgtcggcc cgcagctgca
     7741 tctgtttgca ggtgccgatg cgctggttcc tgcgggggcc gcgccggcgc tgctggaact
     7801 gttgcccgat gtggaaattg gcttaattaa taacgccagc cacgccttcg ccctggagcg
     7861 cccccatgaa gtggcggcgg cgatccaggc ctttttgcat gaggctggcg atgattgatg
     7921 ttgtagggtg cgccgtgcgc accgtggcag aggtcgccca gtgcgcacgg cgggccagcc
     7981 gatgagtgaa gccttccagc ctggcggcct gccggacaaa cgccaggtcg ccgcgtcttt
     8041 ctcccgcgcg gcggaaagct acgacagcgt tgccgccttg cagcgcgatg ttggccggca
     8101 attgctggct cagttgccgg cctcaatcag cccgcaacgt tggttagatc tgggcagcgg
     8161 caccgggtat ttcagccgcg ctttaggcga gcgttttccg gccagccagg gtatggcctt
     8221 ggatattgcc gaaggcatgt tgcagcacgc tcggccactg ggcggcgcgc agcagtttat
     8281 tgctggcgat gccgagcgtt tgccgctgcg cgatgcatcc tgcgagctgc tgttttccag
     8341 tctggcgctg cagtggtgtg cggatttttc cgccgtgctt agcgaagccg aacgcgtgct
     8401 caagcctggc ggtgtgctgg cctttagcag cctgtgtgtt ggcaccctgc aggagttgag
     8461 cgacagctgg catgcggtgg atggctttgt gcacgtcaat cgattccgcc gcttcgagga
     8521 ttatcagcgc ctgtgcgacg ccagcggcct agcgcggctg agcctgcaga caaccccgga
     8581 aaccctctac tacccggatg tacgcagcct gacccatgaa ctgaaagccc tcggcgcgca
     8641 taacctcaac cccggccggc caggcggttt gaccgggcgg gcgcgcatgc tggcgctgct
     8701 caatgcctat gagcggttcc gccagccccg aggcctgccg gcgacctatc aggtggttta
     8761 cggcgtactg cgcaaggaaa ctactagatg agtgcagcgt tttttgtcac cggtaccgat
     8821 accgaaatcg gcaaaaccac catcgccgcc ggcctgctgc atgccgcgcg gctggccggg
     8881 ctgtccacgg cggcggccaa accggtggct tccggttgcg tgcgcacggc ggacggtcta
     8941 cgcaatgacg acgctctggc gctgcttggc gaatgcaccc tggcgctgcg ttatgaagag
     9001 gtcaatcctc tggccttcga gccggcgatt gccccacacc tggctgcccg cgaagccggt
     9061 gtgttgctgg atgttgcggc gctgcaaggg ccggtgcagg caattctgga caagcgtgcg
     9121 gacttgaccc tggtcgaagg cgcgggcggc tggcgcgtgc cgctggccgg gcaggcggcg
     9181 ctgtctgatc tggcgattgc cctgcagttg ccggtgattc tggtggtcgg cgtgcgtctg
     9241 ggctgcatca atcaggcggt gctcacggcc gaagcgatcc tgcgcgatgg cctgccgctg
     9301 gccggctggg tggccaatct gattgatccg caaacctcat gccttgaaga caatctggcc
     9361 actctggccg agcgcctgcc tgcgccttgc ctgggctatg tgccgcgcct ggccgaagcc
     9421 acgccggctg cggtttctgg ctatctcagt ttcggtttat tgcaccgcgc gctataagca
     9481 ggccaagtgc catagccttt tgcttgtacg ttttccgagc aatttctgct tcaattggag
     9541 tgttagtggt tgccatagcg ggagcttggg catggaaatt tctggaagcg ccttcaactc
     9601 gggcatcagc accattcagt ccggccagcg ccgggtcgat caggctgcag cggatatcgc
     9661 cagcaatgcg ctgggtccgc aaccgcagcc aacctctgcg ccgccggcca atcaggttaa
     9721 tcctgcgccc agctcggcag aacaaactcg ccccgatctg gcggaaagtc tggttgccct
     9781 gcgccaaggt cagaatgagg cggaagcggg tgccaaggtg gtgaaaactg ccgatgaagt
     9841 gcttggtact ctgatcgata caacagcttg atacgcttgc ctcgagcgta gtccggctct
     9901 gttgcggagt tttagcagtc aggggcgagg agtcattgca tgcagattgg cggcgcaacg
     9961 ttttatcctg cgcctgcagc aactctactg ccggccaggc ttcttgcgcc caacgtttcc
    10021 cctgatgtgt cccccgatat cactcgcgcc gcccaggcgc cgataaccgc tgctcaaccg
    10081 ctggccgatg agcaatcggc ttctgcgcgt gaagaggctg ataagcctgc cgactcctcg
    10141 gccaatcccg ataaagccaa aagccgcgaa ggcgaaccca gcccgcagca gcaacgtctt
    10201 gagcaactgg aaatcgccaa actggtcagc cgtgatcagg aagtgcgtac ccacgagcag
    10261 gcgcatgctg ccgttggtgg gcgctatgcc ggtgcgccga gttacaccta cgagcgcggc
    10321 ccggatggta agcgctatgc ggtaggcggt gaggtgggca ttgacactag cccgatcccg
    10381 agcgatcccg aagccaccct gcgcaagatg gaggtggtga ttcgcgcggc cctggcaccg
    10441 gccgagccgt ccgcgcagga ccgtcaggtg gcggcgcaag cccaggcgca aatggtcgag
    10501 gcgcgtgccc agctggcgca gcaacagagc agtgaggcgc aggcggccag tgaagcgcgt
    10561 gccgagcggc gcaaggagga tgaggaagag tccgccgagg aagataaatc tgtcgcgcct
    10621 gctcggcaga atctaccgcc gccacctagt cttgatctgt acaagcagct cagcggtttg
    10681 caggcgccag cgccggttgt cgatctggtg gcttgagcgg tgcttggggt gcttgacatt
    10741 aaataggcat aaacgtatgt ttcaaacgct tgtttgatta gctcggttgc caaggggcag
    10801 gcgagcaccg ggattgctcc cgaaaacgac cgaatatccg gtcacctaca ctctaagaat
    10861 ccaccgcaga ggttaaccgc tatgcctgat tacaaggccc ccttgcgtga tatccgtttc
    10921 gtacgcgacg aactgctcgg ctacgaagcg cattatcaga gcctgcctgg ctgccaggac
    10981 gctactccgg aca
//
LOCUS       DASRUM010000042         3659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1861399, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000042 DASRUM010000000
VERSION     DASRUM010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1861399"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..546)
                     /locus_tag="VFF22_03370"
     CDS             complement(43..546)
                     /locus_tag="VFF22_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15892.1"
                     /translation="MTLLTQAVMVRANIDVNRMSAQQKVQLTDEIYVQQPNLLASILV
                     LPRYGVDMQLLEEPIHVLLVAFQAMKHCGHALPTISEDVQETCMQRLTATLKFSEGVP
                     PDLVGQMITKFCVDHPERYLLAFVYGHLGQHDLLRVRTDAEKYLLLACLNLVECVAYI
                     GALLQSK"
     gene            complement(543..2234)
                     /locus_tag="VFF22_03375"
     CDS             complement(543..2234)
                     /locus_tag="VFF22_03375"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HZX15893.1"
                     /translation="MLGTHDLPPLNLQALPPEAAAWVARWQHAEQQLALREQALVDRD
                     RELDQARDLLQRKDRDIAWRDAKIEKITFELARLKRWKFGASSEAMNTDQRRLFEETI
                     AEDEASLRAQLEQLRREAAETTVDAKPKAAPRRPRRQQLPEHLRRVEHRHEPESTNCP
                     SPNCGRPMTRIGEEVSERLDIVPAEFFVHRHIYGKWACRCCQILKQASSNPELIEGGM
                     AASGLIAHTLISRCVDHLPYYRQEAINARSGVHTPRSTLAAWAGQAGAALEPLYEVHK
                     AFVLACRVLHADETPVALLDPGAGKTRKAYVWAYARSWHDPVPGVVYEFCRGRGAQYP
                     KAFLAGDVRRGRGRWSGTLLTDRYTGYDSVLDPRIHADRISAACVAHARRKFEELTRT
                     GTSPIGDEAIRRYARIYAVEAELKGMSDGERMSQRQQLAKPLWAKLKQWLELERRLVA
                     DGSSVAGAIDYTLGHWSALTNYLDDGAVAIDNNHLERQIKPWAMGRRAWLFAGSELAG
                     QRTAMVMSLVQSARLNGHDPWAYLRDVLARLPTQRSSQIEDLLPHRWQAASEAAP"
     gene            complement(2330..2671)
                     /gene="tnpB"
                     /locus_tag="VFF22_03380"
     CDS             complement(2330..2671)
                     /gene="tnpB"
                     /locus_tag="VFF22_03380"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HZX15894.1"
                     /translation="MIRIDAMWLAADPIDMRAGADRLLARVVQVFGAAQAHHGYLFAN
                     ARGTRIKLLVHDGFGVWCAARRLNAGRFAWVPATTTSPISLSLTNAQFDALVLGLPWE
                     RLPELSTITRA"
     gene            complement(2668..3069)
                     /locus_tag="VFF22_03385"
     CDS             complement(2668..3069)
                     /locus_tag="VFF22_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZX15895.1"
                     /translation="MNTIPDQQAGPRQRRRVHSDAFKAGAVAACVQPGMSMAAVAMAQ
                     GINANLLRRWVHEAEMKPRSDVVRADVVVGAAAREPKTVFVPVSLPPSAPPAQIPDIR
                     IELRRGPTTVTVTWPAGAASDCAVWMRELLR"
     gene            3167..>3659
                     /locus_tag="VFF22_03390"
     CDS             3167..>3659
                     /locus_tag="VFF22_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15896.1"
                     /translation="MTAPASPGTRESGAAAGKGATQPGNDSGKEKDKKVVYPEPSNRV
                     KEYREELWRSVFKRVVTNASLVNNRAVLLAICLTHPGHLDAQGLGTDLNAILGGESSH
                     SFGKVLATVLDLREQDLGQAISAIAGNVRSGPMGMGIEDVVSALKALEVKLADHWKVD
                     KGFF"
BASE COUNT          606 a         1164 c         1235 g          654 t
ORIGIN      
        1 cgtgagcgtc cagtcaagcc atcttgaccg gcctcacccg gctcatttgg actgcagtag
       61 tgcaccgatg taggccacgc actccacgag gttgaggcag gccagcagca ggtacttctc
      121 ggcgtcggtt cgcacgcgca gcaggtcgtg ctggccaagg tggccgtata cgaacgccag
      181 gaggtagcgc tcggggtggt cgacgcagaa cttcgtgatc atctgcccga cgagatctgg
      241 cggcacgccc tcggagaact tcaaagtggc tgtcagccgc tgcatgcagg tttcctggac
      301 gtcttcagag atcgttggca aggcgtgccc gcagtgcttc atggcctgga aggcgacgag
      361 caggacgtgg atcggctcct caagcagctg catgtcgacc ccgtagcgag gcaacacgag
      421 gatggaggcc agcagattcg gctgctggac gtagatttcg tcagtcagct gaaccttctg
      481 ttgcgcgctc atgcggttga cgtcgatgtt cgcccggacc atcacggcct gcgtgagcaa
      541 ggtcatggtg ccgcctcgga tgccgcttgc catctgtgcg gcaacaagtc ctcgatctgg
      601 ctgctgcgct gcgtcggcag gcgcgccagc acgtcgcgca ggtatgccca tggatcgtgt
      661 ccattgagcc gcgccgactg caccaggctc atcaccatgg ccgtgcgttg gccagcgagt
      721 tcgctgccgg cgaacaacca tgcccggcgc cccattgccc aaggcttgat ctgccgctcc
      781 aggtggttgt tgtcgatcgc gactgcgccg tcatcgaggt agttcgtcag cgcgctccag
      841 tggccgagcg tgtagtcgat ggcgcccgcc acggagctgc cgtccgcgac cagccgtcgt
      901 tcgagctcga gccattgttt gagcttggcc cacagcggct tggcgagttg ctggcgctgc
      961 gacatgcgct cgccgtcgct catgcccttg agttcggcct cgaccgcata gatccgcgca
     1021 tagcgacgga tcgcctcgtc gccgatcggg ctcgtgccgg tgcgagtcag ctcctcgaac
     1081 ttacggcgtg catgcgcgac gcaagccgcg ctgatgcgat ctgcatggat gcgcgggtca
     1141 agcacgctgt cgtagcctgt gtagcggtcc gtcagcagcg ttccggacca gcgccctcgg
     1201 ccccgtcgca catcgcccgc gaggaaggcc ttcgggtact gcgcgccacg gccccggcag
     1261 aactcataga ccacgccggg cacggggtcg tgccagctcc tcgcgtaggc ccagacgtag
     1321 gccttgcgcg tcttgccggc accggggtcg agcagcgcca cgggcgtctc gtcggcatgc
     1381 agaacccggc aagcgaggac gaaggccttg tgcacctcgt agagcggctc cagcgccgcg
     1441 cccgcctggc ctgcccaagc cgccagcgtg gaacgcggtg tgtggacgcc gctgcgggcg
     1501 ttgatcgcct cttgcctgta gtacggcagg tggtcaacgc agcggctgat gagcgtgtgc
     1561 gcgatgaggc cgctggcggc catgccgccc tcgatcagtt ccgggttgga cgacgcctgc
     1621 ttgaggatct ggcagcagcg gcaggcccac ttgccgtaga tgtggcgatg gacgaagaac
     1681 tcggccggca cgatatccag ccgctcgctc acctcctcgc cgatgcgtgt catcgggcga
     1741 ccgcagttag gcgaggggca gttggtgctc tcgggctcat gacggtgttc gacgcgacgc
     1801 aggtgttcag gcaattgctg acggcgtggt cgccgtggcg cggccttcgg cttggcgtcg
     1861 acagtggtct cagcggcttc gcgtcgcagt tgttccagct gcgcacgcag gctcgcctcg
     1921 tcctcggcga tcgtctcctc gaacaggcgt cgctggtcgg tgttcatcgc ctcggagctg
     1981 gcgccgaact tccagcgctt caggcgagcc aactcgaagg tgatcttctc gatcttggca
     2041 tcgcgccagg cgatgtcgcg gtccttgcgc tgcaggagat ccctggcttg gtccagctcg
     2101 cggtcgcgat cgacaagcgc ctgctcacgc agggcgagtt gctgttcggc atgctgccaa
     2161 cgggcaaccc aggccgccgc ttcgggcggg agtgcctgca ggttgagcgg gggaagatcg
     2221 tgcgtgccga gcatgacggc atggtgccag gacgccgtat gcagcgcatc ggaacaagta
     2281 cgaattgcgt cgacgctggc tcggtcaagt gccagcgtcg atgttgctgt caggcccgcg
     2341 tgatggtgct caattcgggc aaccgctccc acggcaggcc gaggaccagc gcgtcaaact
     2401 gcgcgttggt gagcgacaga gatatgggcg atgtcgtggt ggccggtacc cacgcgaagc
     2461 ggcccgcgtt gagccggcgt gccgcgcacc agacgccgaa gccgtcgtgc acgaggagct
     2521 tgatgcgggt gccgcgggcg ttggcgaaca ggtagccgtg gtgggcctgc gctgcaccga
     2581 agacttgcac aacgcgcgcc agcaggcggt cggcgccggc gcgcatgtcg atggggtcgg
     2641 cggccagcca catcgcgtcg attcggatca tcgaagcagc tcgcgcatcc agaccgcgca
     2701 gtcgctggcg gcacctgccg gccaggtcac cgtgaccgtc gtcggtccac gtcgcagctc
     2761 gatgcgaatg tctgggatct gagccggtgg cgccgatggc ggcaagctga ccggcacgaa
     2821 gacggtcttg ggctctcgcg ctgctgctcc aaccacgacg tctgcgcgaa cgacatcgct
     2881 tcttggcttc atctcggcct cgtgcaccca gcgccgcaga agattggcgt tgatgccctg
     2941 agccattgcc actgcggcca tcgacatgcc aggctgcaca caggccgcca ccgcaccggc
     3001 cttgaacgca tcgctgtgca cgcgccgccg ctggcgcgga cctgcttgtt gatcggggat
     3061 agtgttcacg agtccaccaa gagttgaagt ggactcgagg ctcctatgaa ccgccccggc
     3121 tttcaagatg ggctgactgg acgctcacca gcaggccgcc aaccaggtga cagcacccgc
     3181 aagccctggc actcgtgaga gtggtgcggc ggccggaaag ggggcaaccc agcccggcaa
     3241 cgactcgggc aaggaaaagg acaagaaggt ggtctacccc gagccgtcga atcgcgtgaa
     3301 ggaataccgg gaagagttgt ggcgcagcgt gttcaagcgc gtcgtcacca acgcttctct
     3361 ggtcaacaac cgggctgtgc tgctggccat ttgcttgacg cacccagggc atctcgatgc
     3421 tcaagggctc ggcaccgacc tcaacgcaat cctgggtgga gagagctcgc attccttcgg
     3481 gaaggtgctt gcaaccgttc ttgacctgcg tgagcaggat ctgggacagg ccatcagtgc
     3541 gattgcaggc aacgtccgct ccggccccat gggcatgggc atcgaagacg ttgtgagtgc
     3601 actcaaggcc ctcgaagtga agctcgccga ccactggaag gtggacaagg gctttttcg
//
LOCUS       DASRUM010000043         5396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_359181, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000043 DASRUM010000000
VERSION     DASRUM010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359181"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VFF22_03395"
     CDS             complement(<1..140)
                     /locus_tag="VFF22_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15897.1"
                     /translation="MAGKTLYDKLWEMHEVKRRDDGSSLIYIDRHILHEVTSPQAFEG
                     LRL"
     gene            321..1214
                     /locus_tag="VFF22_03400"
     CDS             321..1214
                     /locus_tag="VFF22_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX15898.1"
                     /translation="MDLATLNAFIAIAELGSFSEAAERLHLTQPAVSKRIASLEQQLS
                     VRLFDRLGREVSLTEAGRALLPRAYQILNVLDDTRRALTNLNGEISGRLTLATSHHIG
                     LHRLPPLLRAFTRAHPQVALDIQFLDSEVAYEEVLHGRAELAVITLAPETREPVHAVA
                     VWDDPLDFVAAPEHPLARSQVISLADVAHHPAVFPGGNTFTHHIVRRLFEAQGLTPNI
                     AMSTNYLETIKMMVSIGLAWSVLPRTMLDEQVARLPLPGIQLTRQLGYISHTERTLSN
                     AARAFMNLLDAQRDGLAMARD"
     gene            1318..4617
                     /locus_tag="VFF22_03405"
     CDS             1318..4617
                     /locus_tag="VFF22_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008100301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZX15899.1"
                     /translation="MAKSSDRRPPLPFIPALDPAEFESTWNDAPRLLRALNGAKLGTW
                     YWDIESGRVSWSRGAQALFGLDPKQPLEKRIDYIELIPEEDRPDILRLFEQLLAGNPS
                     NSAFRHRIRWPDGSLHWLEISGSLQLDADGKQRVIGVIRDISSQQAREQALRSSEEKF
                     AQAFKYSPDAVVITEKSSGRFIEINPGFERQFGWTSEQTLGRTSLEMGIWARLKDRQR
                     MMDAIKNNTLNGLEVPLYHRDGSIRRNLLFGGEVLLEGIPCLVLSLRDITQQRQQEQA
                     LHDSQERLNLALDSADLGTWDWHIPSNMLFGSARASKLHGLADEPFHDDFRKFFSFVP
                     LEDRHAMRKRFNNLAQGLTEDYQVTYRAVYSSQEVHYLESTAKLYRDADGKPERMAGI
                     LIDISERVRREQSLAASEAKFANLFQASPDPVALTTLPDGVAMEINTSFSQTFGWQPE
                     EVIGRRMIDLNFWADLEVRAALYDKLERDHSLHNEIADYLDKHGRLITCVVSSRPMML
                     NNQRCILTTFRDISERQKAQAELQASQEKFALAFHSSPDAITITERDSGRYIEVNEGF
                     GRLTGYNIDEVIGRSAMELNVWADLSERDLMVQKLQRDGHVYHMEMRGKHRDGTIKLV
                     EVSVEPIELNKTPCLLLTARDISELKAAQAQVQHLAYHDSLTNLPNRALLLDRLTQQI
                     SLLRRHELRGALLFIDLDHFKHINDSLGHPVGDAVLKLVTARLEASVRQEDTVARLGG
                     DEFVVLISGLEGKRSKVVQLVRALAEKLRHLLAEPMLLDGHQLQVTPSIGIALIPDHG
                     DTPSDLLKRADIALYRAKDSGRNCIQLFRNTMQKAASERLRLENDLRLALARGEFELH
                     FQPQVDARNNQIIGAEALLRWMHPTLGAQSPALFIHVLEESGLILEVGGWVLTEACHA
                     CAQLLEQGMVNAAQFSLCVNISPRQFRQNDFVELVERSLTSSHLPPHMLKLEITEGIV
                     IQNIDDTIAKMNRLKRLGVTFAMDDFGTGYSSLTYLKRLPVDVLKIDQSFVRDATLDA
                     NDAEIIRAIVAMARSLGLDMIAEGVEQTDQLEFLQQQGCHHYQGYLFSRPVPLEQFRQ
                     LLRDNHTPTPR"
     gene            complement(4666..5112)
                     /locus_tag="VFF22_03410"
     CDS             complement(4666..5112)
                     /locus_tag="VFF22_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HZX15900.1"
                     /translation="MSTAFSMTPLFRQSVGFDRFNDLFESAMRNEAGSSYPPYNVEKY
                     ADDQYRIVVAAAGFQEDDLELQVERGVLTVTGGKRETSAENVTYLYQGIAQRAFKLSF
                     RLADHIEVKAASLVNGLLNIDLVRIVPEEAKAKRIPIGASRTVLQG"
     gene            5245..>5396
                     /locus_tag="VFF22_03415"
     CDS             5245..>5396
                     /locus_tag="VFF22_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZX15901.1"
                     /translation="MKKVILITGASRGIGAATARLAAAQGYALCLNYHRQQAAAEALV
                     SEITLAG"
BASE COUNT         1151 a         1759 c         1508 g          978 t
ORIGIN      
        1 agacgcaagc cctcaaaggc ctggggcgag gtcacttcgt ggaggatatg ccggtcgatg
       61 tagatcagcg acgaaccatc gtcgcggcgc ttcacttcgt gcatttccca gagcttgtcg
      121 tacagcgttt tgccggccat ccgctatccc tcatcaacct gtttctatgc ctgggcaaat
      181 agccctttgg cttatggggt aatcctatgg gcttgcatta aattactcaa attcatattt
      241 ttcatccaaa ggattcctaa ctggaataca agtaagcttg agcagcgcct attgcgccgc
      301 ccactgccag gaacagccac atggatctcg ccaccctcaa tgcctttatt gccatcgccg
      361 agctgggcag cttctccgaa gcggcggaac gcctgcacct gactcaacca gcagtgagca
      421 aacgcatcgc cagcctggag cagcagctga gtgtgcgcct gttcgatcgc ctgggccgtg
      481 aggtcagcct gaccgaggcc ggccgcgcct tactgccgcg ggcctatcag attctcaatg
      541 tgctggatga tacgcgccgt gcgctgacca atctgaatgg cgagataagc ggccgtctga
      601 ccctggccac cagccaccac attggcctgc atcgcctacc accgctgcta cgcgccttta
      661 cccgcgcgca cccgcaggtg gcgctggata tccagtttct cgactccgag gtggcctatg
      721 aagaagtgct gcatggtcgc gctgagctgg cggtgatcac cctcgctccg gaaacccgcg
      781 agccggtgca tgccgtggcc gtgtgggacg acccgctgga tttcgtcgcc gctcccgagc
      841 atccgctggc acgcagccag gtcatcagcc tggccgatgt agcacatcac ccggcagtat
      901 tccccggcgg caacaccttt acccaccata tcgtgcgacg ccttttcgag gcccaagggc
      961 tgacgccgaa tatcgccatg agcaccaact acctggagac catcaagatg atggtctcca
     1021 taggcctggc ctggagcgta ttgccgcgca ccatgctcga cgaacaggtg gcgcgcctgc
     1081 cattaccggg tatccagctg acccgccagc taggctatat cagccacacc gagcgcaccc
     1141 tctccaatgc cgctcgggcc tttatgaacc tgctggatgc ccagcgtgac ggccttgcaa
     1201 tggccaggga ctaagggcta acgtgggttt ataacgataa gaaaggtttc tgcatgactc
     1261 agcccagctg ttcagccaaa ggccatgaaa ccgacgcaga tcaaggaaga gtgggtcgtg
     1321 gctaaatcca gtgatcgccg tccaccactg ccgtttatcc cggcactgga ccctgccgag
     1381 ttcgaaagca cctggaacga tgccccacgc ctgttacgcg ccctcaacgg tgccaagctg
     1441 ggcacctggt actgggacat tgaaagcggc cgagtcagtt ggtcacgcgg cgcccaggcc
     1501 ctgtttggcc tcgaccccaa gcagccgctg gaaaaacgca tcgactatat tgagctgatc
     1561 cccgaggaag accgccccga tatactgcgc ctgttcgagc aactgcttgc cggcaacccg
     1621 agcaacagcg cctttcgcca ccgcatccgc tggccggatg gcagcctgca ctggctggaa
     1681 atcagcggca gcctgcagct ggatgccgat ggcaaacagc gcgtcattgg cgtaatccgc
     1741 gacatcagca gccagcaggc ccgcgagcag gcgctgcgca gcagcgagga aaaattcgcc
     1801 caggcgttca aatacagccc ggatgccgta gtcatcaccg agaaatccag cggccgtttt
     1861 atcgagatca accctggttt tgaacgtcaa tttggctgga ccagcgaaca gaccctgggc
     1921 cgcacgtccc tggagatggg gatctgggcc cgccttaaag atcgccagcg gatgatggat
     1981 gcgatcaaga acaacaccct caacggcctt gaagtaccgc tttatcaccg cgacggcagc
     2041 atccgcagaa acctgctgtt tggcggcgaa gtccttctag aaggtatccc ctgcctggtc
     2101 ctgtcgttgc gtgacattac tcagcaacgc cagcaggagc aggccctgca cgacagccag
     2161 gagcgcctca acctggccct agactccgcc gacctcggca cctgggactg gcacatcccc
     2221 agcaatatgc tgttcggctc ggcccgcgcg tcaaaactgc acggcctggc ggacgagccc
     2281 ttccatgacg actttcgcaa gttcttcagc ttcgttccgc tggaagatcg ccatgccatg
     2341 cgcaagcgct tcaataacct agcgcagggc ctgaccgagg actaccaagt cacctaccga
     2401 gcggtgtaca gcagtcagga agtgcactac ctggaaagca ctgccaagct ctaccgtgat
     2461 gccgacggca agcccgagcg tatggccggc atccttatcg atatcagcga acgggtgcgc
     2521 cgcgagcaga gcctggccgc ctcggaagcc aaattcgcca acctgttcca ggccagccca
     2581 gaccccgtag ccctgaccac cttgccagat ggcgtggcca tggaaatcaa caccagcttc
     2641 agccagacct tcggctggca acccgaggaa gtcatcggcc ggcggatgat cgacctgaac
     2701 ttctgggccg accttgaggt gcgtgcagcg ctatacgaca agctcgaacg cgaccacagc
     2761 ctgcacaacg aaatagcgga ttacctcgac aaacacggcc gactgatcac ctgcgtggtc
     2821 tcatccaggc cgatgatgct gaataaccag cgctgcatcc tcaccacctt tcgcgatatc
     2881 agcgaacggc agaaggccca ggccgagctg caggccagcc aggaaaagtt cgccctggcg
     2941 ttccactcca gccccgacgc catcaccatc accgaacgag acagcggccg ctatatcgaa
     3001 gtcaacgaag gcttcggccg cctgactggc tataacattg atgaggtgat cggtcgcagc
     3061 gccatggaac tcaacgtctg ggcggacctg tccgagcgcg atttgatggt gcagaagctg
     3121 cagcgcgacg gccatgtcta tcacatggaa atgcgcggta agcaccgcga tggcacgatc
     3181 aagctggtcg aggtatcggt cgaacccatc gaactcaaca aaaccccctg cctgctgctc
     3241 accgcccgcg acatcagcga gctcaaggca gcccaggccc aagtgcagca cctggcctac
     3301 cacgactcgc tgaccaacct gccgaaccgc gccctgctgc tcgaccgcct gacccagcag
     3361 atctccttac tcaggcgtca cgaactgcgc ggcgccctgc tgtttatcga tctcgatcac
     3421 ttcaaacaca tcaatgattc gctcggccat ccggtgggcg atgccgtgct caagctggtc
     3481 accgcacgcc tggaagccag cgtgcgccag gaagacaccg tggcgcgcct gggcggcgac
     3541 gaatttgtcg tgctgatatc cggcctggaa ggcaaacgct caaaggtggt gcaactggtt
     3601 cgcgcgctgg cagaaaaact gcgccacctg ctggccgaac cgatgctgct cgacggccat
     3661 caactgcaag tcaccccgag tatcggcatc gccctgattc ccgaccatgg cgacacccct
     3721 tcggacctgc tcaagcgcgc ggacatcgcc ctctatcggg ccaaggattc cgggcgcaac
     3781 tgcatccagc tgtttcgcaa caccatgcag aaggccgcca gcgaacgcct gcgcctggaa
     3841 aacgacctgc gcctggccct ggcccggggt gaattcgaac tgcactttca accccaggtg
     3901 gatgcgcgca acaaccagat catcggcgcc gaagccctgc tgcgctggat gcacccgacc
     3961 ctgggcgcgc agtcgccggc actgtttatt catgtgctcg aagaaagcgg gctgattctc
     4021 gaagtcggcg gctgggtgct caccgaagcc tgccacgcct gcgcccaact gcttgagcag
     4081 ggtatggtca acgccgcgca gttcagcctg tgcgtgaata tcagcccccg gcagtttcgc
     4141 cagaatgact ttgtcgagct ggtggaacgc agcctgacca gcagccacct gccaccgcac
     4201 atgctcaagc tggaaatcac cgaaggcatc gtgatccaga acatcgacga caccatcgcc
     4261 aagatgaacc gcctcaaacg cctcggcgtg acattcgcca tggatgattt cggcaccggt
     4321 tacagctcgc tgacctacct caagcgcctg ccggtggatg tgctgaagat cgaccagtcc
     4381 ttcgtgcgcg acgccaccct ggatgccaac gatgcggaaa tcatccgcgc catcgtcgcc
     4441 atggcgcgca gcctgggcct agacatgatt gccgaaggcg tcgagcaaac cgaccagctg
     4501 gagtttctcc aacaacaggg ctgccaccat taccagggct acctgttcag ccggccggtg
     4561 ccgctggagc agttccgcca gttgctgcgc gacaaccaca caccgacgcc ccgctaaaac
     4621 aacaagggca cccgtaggtg cccttgttac tagccgagtg gttgtttaac cctgcagcac
     4681 agtgcggctg gcaccgattg gaatacgctt ggccttggcc tcttccggca cgatgcgcac
     4741 caggtcgata ttcagcaggc cgttgaccag gctggcagcc ttgacctcga tatggtcggc
     4801 caggcggaac gacagcttga acgcacgctg ggcgatgccc tggtacaggt acgtgacgtt
     4861 ctcggcgctg gtttcgcgct tgccgccggt aacggtcagc acaccgcgct cgacctgcag
     4921 ctccaggtca tcttcctgaa agcccgccgc cgcgaccaca atgcggtatt ggtcgtcggc
     4981 atacttctcg acgttgtagg gtggatagct gctgcccgcc tcattgcgca tggccgactc
     5041 gaacaggtca ttgaaccggt cgaagccaac ggactggcgg aacagcgggg tcatggaaaa
     5101 tgcggtgctc atgatttaaa tctcctgaaa ttcagcgagt ggttaatcgc ggaacccgac
     5161 ttcggcatcc cgcatgacta aaaatgtggc tactcaacta gatttcaagc ctctgcaaaa
     5221 ttttcttaca gggaggagac actgatgaaa aaggtcatac tgatcaccgg cgccagtcgc
     5281 ggcattggcg ccgccactgc cagactggcg gccgctcagg gctacgcgct gtgcctgaat
     5341 tatcaccggc agcaggcagc ggctgaggcg ctggtgagcg agatcaccct ggcggg
//
LOCUS       DASRUM010000044         4069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2365498, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000044 DASRUM010000000
VERSION     DASRUM010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2365498"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VFF22_03420"
     CDS             <1..365
                     /locus_tag="VFF22_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HZX15902.1"
                     /translation="CTGGRIVHHFKHNLWRENCHLIFPGFQAKGTLGRNIVDGAESVK
                     FLHQRIAVKAKVHTLGGFSAHAGQSQLLDWVSHFEHHPELYLVHGELEKMQALQLALR
                     KRLNWIANIPEPGEQIAL"
     gene            499..1578
                     /locus_tag="VFF22_03425"
     CDS             499..1578
                     /locus_tag="VFF22_03425"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HZX15903.1"
                     /translation="MPYESDDFLSRHFQTSGADLTTRVEELGILAAPPDSPNTKLYQA
                     MLGTVVRMAQADRNRWDAKIMLQTLREMEHAFSALEQFKRRRKVTVFGSARTPPAHPV
                     YRLARDLGATLAKHNLMVITGAGGGIMAAAHEGAGLENSLGFNITLPFEQGANATVQG
                     SNNLLSFHFFFLRKLFFVKEADGLVLCPGGFGTLDEALEVLTLIQTGKSPLVPVVLLD
                     QPGGTFWSSALTFLQEQLQNNGYILPSDMRLIRLVHSAEDAAEEIAQFYRNYHSSRWL
                     KERYVIRLNHELSEAAMQQLNDEFRDLCIQGDFQQQARCESEQDEPELCHMTRLAFAF
                     NGRNHGRLRELLDLVNQPQNWAEKA"
     gene            complement(1777..2241)
                     /gene="recX"
                     /locus_tag="VFF22_03430"
     CDS             complement(1777..2241)
                     /gene="recX"
                     /locus_tag="VFF22_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010451268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HZX15904.1"
                     /translation="MSLALDNPVAVRRAAMDLLARREHGRVELTRKLRSRGAADDLIE
                     AALDRLAEEGLLSEARYLEAFVSYRARSGYGPLRIREELTQRGLARGDVEQALRDSGI
                     DWCEHLQETWQRKFAGELPRDARERAQQGRFLSYRGYPPELIGRLLRGALDD"
     gene            complement(2253..3296)
                     /gene="recA"
                     /locus_tag="VFF22_03435"
     CDS             complement(2253..3296)
                     /gene="recA"
                     /locus_tag="VFF22_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HZX15905.1"
                     /translation="MDENKKRALAAALGQIEKQFGKGAVMRMGDHDRQAIPAISTGSL
                     GLDIALGIGGLPKGRIVEIYGPESSGKTTLTLSVIAEAQKLGATCAFVDAEHALDPEY
                     AGKLGVNVDDLLVSQPDTGEQALEITDMLVRSNAIDVIVIDSVAALVPKAEIEGEMGD
                     MHVGLQARLMSQALRKITGNIKNANCLVIFINQIRMKIGVMFGSPETTTGGNALKFYA
                     SVRLDIRRTGAVKEGDEVVGSETRVKVVKNKVAPPFRQAEFQILYGKGIYRNGEIIDL
                     GVQHGLLEKSGAWYSYQGNKIGQGKANSAKYLEDNPEVARTIEGLIREKLLVVSPTVK
                     ANSTADDLADADA"
     gene            complement(3387..3899)
                     /locus_tag="VFF22_03440"
     CDS             complement(3387..3899)
                     /locus_tag="VFF22_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HZX15906.1"
                     /translation="MTANEYALTQLAAELGGQLIAAKAQVTTAESCTGGGIAEAITRI
                     PGSSAWFEAGYVTYSNAQKTKQLSVPEALFVTVGAVSQEVVEAMVRGAQAHSGARFAV
                     AVSGVAGPDGGSAEKPVGTVWLAWGDGAQLFSVRRQFAGDRSEVRRQTVEAALAGLLR
                     LAAEENPNAG"
BASE COUNT          855 a         1310 c         1130 g          774 t
ORIGIN      
        1 tgtgcaccgg cgggcgcatt gtccatcact tcaagcacaa cctctggcgc gagaattgcc
       61 acctgatctt tcctggcttc caggccaagg gcacgttggg ccgcaatatc gttgacggcg
      121 ccgagagcgt gaaattcctc caccagcgca tcgcggtaaa agccaaggta cacaccctcg
      181 gcggcttctc cgcgcacgcc gggcagtcgc agctgctcga ctgggtcagc cacttcgagc
      241 accaccccga gctgtacctg gtgcatggcg aattggagaa gatgcaggcc ctgcaactgg
      301 ccttgcgcaa gcgtttgaac tggatcgcca acatccctga accaggcgag caaatcgccc
      361 tctgatgcag tcctggcata ggctggtggt gatcacacag gtctatgtgc cgcacaaacc
      421 atcagccagc agccatactc aggacaaggc cgatgcagca gtacgctgca tcggcaggca
      481 accagaagga gaagttgtat gccttatgaa tcggacgatt ttctgtcccg gcattttcag
      541 accagtggcg ccgatctgac cacccgcgtg gaagaactgg gcatccttgc cgcaccgccc
      601 gacagcccca ataccaagct ctaccaggcc atgctcggca cggttgtacg catggcgcag
      661 gctgatcgca accgctggga tgccaagatc atgctgcaga ccctgcgtga aatggagcat
      721 gccttcagcg cactggaaca gttcaagcgg cggcgcaagg tgaccgtgtt cggctcggca
      781 cgcaccccgc ccgcccaccc ggtttaccgc ctggcccgcg acctcggcgc gaccctggcc
      841 aaacataatc tgatggtcat caccggtgcc ggcggcggca ttatggcggc ggcccacgaa
      901 ggtgctggcc tggaaaacag cctgggcttc aatatcaccc tgcccttcga gcaaggtgcc
      961 aatgccaccg tacaaggcag caacaacctg ctgtcgttcc acttcttctt tctgcgcaag
     1021 ctgttcttcg tcaaggaagc cgatggcctg gtgctctgcc cgggcggctt cggcaccctg
     1081 gacgaagcgc tggaagtgct gaccctgatc cagaccggta aaagcccgtt agtacccgtg
     1141 gtattgcttg accagcctgg cggcaccttc tggagcagtg ctctgacctt tctgcaggaa
     1201 cagctgcaga acaacggcta tatcctgccc agcgacatgc gcctgatacg cttggtgcac
     1261 agcgccgaag atgccgccga agaaatcgcc cagttctacc gtaactacca ctccagccgt
     1321 tggctcaagg agcgctacgt tatccgcctc aaccatgagt tgagcgaagc ggccatgcag
     1381 cagctgaacg atgagttccg cgacctgtgc atacaaggcg acttccagca gcaagcgcgc
     1441 tgtgaatccg agcaggacga accggagctc tgccatatga cgcgcctggc cttcgccttt
     1501 aacggccgca accacggccg cttgcgcgaa ttgctcgacc tggtcaatca gccgcagaac
     1561 tgggcggaaa aagcctgatc tgttgcccag ccgatagcct gctgcaggct atcggcaatc
     1621 gcgacttcac tggttgacgg gaaaagatcg gctacacgca tttcagcaca gaaagctttt
     1681 gcctccagtg ctgcagcatc atcggcgtgt gggagtaggt aacggggaga acggagggag
     1741 ttgatacaaa ctggtcggcg aacagcaagc ttcggcttaa tcgtccagcg caccgcgtag
     1801 caagcgtcca atcagctcag gcggataacc ccggtaactg agaaagcgcc cctgctgggc
     1861 cctctcacgg gcatcacggg gtagctcacc ggcaaacttg cgttgccagg tttcctgcag
     1921 atgttcacac cagtcgatac cactgtcgcg caaagcctgc tcaacatcac cacgggccag
     1981 gccgcgctgg gtcaactctt cgcgaatacg caacggccca tagccggaac gcgcccggta
     2041 gctgacaaag gcttccagat aacgcgcctc agacagcaag ccttcttcag ctaggcgatc
     2101 aagagcggct tcgatcaagt catcagccgc tccacgactg cgcaacttgc gggtcaactc
     2161 gacgcggcca tgctcacgcc gcgccagcag atccatggcc gcccgtcgca ccgcgacggg
     2221 gttatccaga gcaagggaca taggcagaag aatcaggcgt cggcgtcagc cagatcatcg
     2281 gccgtggagt tggccttgac cgtcgggctg accaccagca atttctcgcg gatcaaacct
     2341 tcgatggtgc gtgccacctc aggattgtct tccaggtact tggccgagtt ggccttgccc
     2401 tggccgatct tgttgccctg atagctgtac caggcacccg atttttccag caggccgtgc
     2461 tgcacgccga ggtcgatgat ctcaccgttg cggtagatgc ctttgccgta aagaatctgg
     2521 aactcagcct gacggaacgg cggagctacc ttgttcttca caaccttgac ccgggtttcg
     2581 ctacccacca cttcatcgcc ttccttcacc gcgccggtac ggcgaatgtc caagcgaaca
     2641 gaagcgtaga acttcaacgc gttaccaccg gtggtggttt ccgggctgcc gaacatcacg
     2701 ccgatcttca tgcggatctg gttgatgaaa ataaccaggc agttggcgtt cttgatgttg
     2761 ccggtgattt tgcgcagcgc ctggctcatc aggcgagcct gcaggccaac gtgcatatcg
     2821 cccatttcgc cttcgatttc cgccttcggc accagagccg ccacggagtc gatcacgatc
     2881 acgtcgatgg cattggagcg caccagcatg tcggtgattt ccagggcctg ctcgccggta
     2941 tccggctgcg aaaccagcag atcgtcaacg ttgacgccca gcttgccagc gtactcaggg
     3001 tcgagcgcat gctcggcatc gacgaaggcg caagtggcac cgagcttttg cgcttcggcg
     3061 atcaccgaca gggtcagggt ggttttaccg gaagactcag ggccgtagat ttcgacgata
     3121 cggcctttcg gcagaccacc aatacccagc gcgatgtcga gacccagcga gccagtggaa
     3181 atggccggga tcgcctggcg atcatgatcg cccatgcgca tgaccgcacc tttgccgaat
     3241 tgcttttcaa tctggcccag ggctgccgcc aaggcgcgct tcttattctc gtccatttaa
     3301 gtcctcacgt aatcaagaag ggccagctag cggccaccaa caactgtata tgtagccagt
     3361 attattccac agggcaagtc gcccgcctac cccgcatttg gattttcttc cgcggccagc
     3421 cgcaatagcc ctgccagtgc ggcctcgacc gtttgtcggc gaacttcgct gcggtccccg
     3481 gcaaactgcc ggcgcacgct gaatagctgc gcgccatcgc cccatgccag ccagaccgtg
     3541 cccaccggtt tctccgccga gcccccgtcc ggtcccgcca cgccgctgac cgccaccgca
     3601 aagcgagcac cactgtgagc ctgagcacca cgcaccatgg cctcgaccac ctcctgactc
     3661 actgccccaa cagtgacgaa cagcgcctcg ggcacgctca gctgtttagt cttctgcgca
     3721 ttggagtaag tgacataacc ggcctcaaac caggccgagc tgccgggaat ccgggtaatc
     3781 gcctcggcaa tgccgccacc ggtgcaggat tcggctgtgg tcacctgggc cttggcggca
     3841 atcagttgcc cgcccagctc ggcagccagt tgagtcagag catattcatt agcagtcatc
     3901 tgcactccgg ggttaaagcc agctaggccg ataccgtaca ctaggcgctt ttgcgattca
     3961 gccggacagt taaaacgatg agccaaagcg acctcagttc tcacacgccg atgatgcagc
     4021 agtactggaa gctgaaaaat cagcacccgg aacagctgat gttttaccg
//
LOCUS       DASRUM010000045         7025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2031156, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000045 DASRUM010000000
VERSION     DASRUM010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7025
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2031156"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..472
                     /gene="rnk"
                     /locus_tag="VFF22_03445"
     CDS             59..472
                     /gene="rnk"
                     /locus_tag="VFF22_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HZX15907.1"
                     /translation="MTSAPSLTITRLDLQRLEHLLDSLDDFGPSAIALQAELDRADVV
                     GHDEVPAGVVTMNSRVHCREESSGKDYHLTLVYPQDAGGEGCVSVLAPVGTALLGLSV
                     GQHIDWAAPGGKVLKLTLLAVEYQPEAAGEYSRAE"
     gene            530..1330
                     /locus_tag="VFF22_03450"
     CDS             530..1330
                     /locus_tag="VFF22_03450"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HZX15908.1"
                     /translation="MSSLPMYFPQGFDLSRALLCAQLVSSAYDQYSQWQAQQRPRRPQ
                     DFTWQPPTLPGWGFSAPIWSVLSELHFLNESEPFGFAARDPQGEVYLVFRGTDTVQDW
                     LDDLDADQRAYPWQPGLGNVHDGFLKLYTSLRDQALQAVDTQQPNGPLWVCAHSLGGA
                     LSSLAVLDMRERWPDLPLQHYSFASPRLAAPDFAAYYNGLQVPTFRVVNDSDLVPEVP
                     PGVTGKWLYQHLGLAVTFSASYASVGDNHSLAGCYLYALQNPQAPMRC"
     gene            complement(1331..4186)
                     /locus_tag="VFF22_03455"
     CDS             complement(1331..4186)
                     /locus_tag="VFF22_03455"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HZX15909.1"
                     /translation="MSRTEEIRPDIEDGIDRKVLTQLRARFLKLNQGRLQRAMQALST
                     RQQLVLKLLPLLFHVNHPLLPGYVSGLTPAGLSGFEPDDELLSEVQRLTRSFAYKPYR
                     GKQALPIHGLFLMGSLGTVAQAEQSDMDLWVCHASDLSTQQLHELNKKCALLESWAAT
                     FGAEAHFFLIDPARFTQGEREAQLTSDDCGTTQHYLLLDEFYRTAIWLGGRTPLWWLV
                     PVYEEQRYQHYTSTLLAKRFIRAEDVLDLGHLARIPPEEFIGAGMWQLFKGIESPYKS
                     VLKLLLTEVYASEHPKVECLSLRFKQAVFANRLDVDELDPYIVVYRRLEEYLKARGEH
                     ERLELIRRCLYLKVDKKLSRPPRNNRKSWQRLLLERLTRDWNWDSRQLAMLDSRSQWK
                     VRQVSAERRMLVNELTYSYRFLSEFARSEHAGSSLNSRDLGILGRRLYAAFERKAGKI
                     EFINPGIAPDLAEDTLTLAQSPSPDEPNETLWALYSGSVNAQDLEDFSPLKRSRELIE
                     LLAWCQRNGVIDNSTRLSLHPGSSDLTEYELSNLLSSLQQVVPLPLPAVDEAALLSAS
                     VPGEVLLLVNVGIDPLKQFSQMNMHMTTERTDALGYSGVRENLILTIDQVTLNSWNEL
                     LVTRYDGPHALLDCLRDFLNNLPASGRKPNLRVRCFCRNRATAIAERVEELFRETLGS
                     FSSEQANRYLLQIKQQYHVLELKPGHVSHTSLKDLPTLIDHLGEELPGYSELHLDRSA
                     LEGEDLALILPVGRPQCIQVFYRLNEASATAELSVLDEHNALWRQQMPFRDEQSLLTP
                     LQRFLQSVLYRRNALLPLDNLPAHQPLEVLYYQVLPDAPLRAQRLERRPPPLSPISHP
                     FYDVQAIVEPADHKRSHVTLYCNHREFSELEYAGDLFAAVAQHILAQRRDGARYPCYI
                     TDLDLSAVLGDGHMQSVHYLRYKSRLEAALNQALQKA"
     gene            complement(4364..4609)
                     /locus_tag="VFF22_03460"
     CDS             complement(4364..4609)
                     /locus_tag="VFF22_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02647 family protein"
                     /protein_id="HZX15910.1"
                     /translation="MAFTPELIAELEILALFNLGTTQEGIKVHHTATPSAIAAAQRLH
                     DKGLINQPDGGYLTSLGLDAAEHAQTLLTILNVTEAA"
     gene            complement(4787..5056)
                     /locus_tag="VFF22_03465"
     CDS             complement(4787..5056)
                     /locus_tag="VFF22_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15911.1"
                     /translation="MSEQEDEDFAQDTLIQAIENQLEAGEPAFVQAVLNKLSLVGYER
                     EEIIELMALVLADEIDVMLRDDRPFDLTRYEQGLRALPELPEQAP"
     gene            complement(5080..5559)
                     /locus_tag="VFF22_03470"
     CDS             complement(5080..5559)
                     /locus_tag="VFF22_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX15912.1"
                     /translation="MPLQIRTATIEDTAQILRFIIDLAIYEKAEHEVKTDAAGIQNSL
                     FAANSTSHGLICELDGVPVGYAVYFFSYSTWLGKHGIYLEDLYISPEHRKIGAGKALL
                     RHLAQLAVSKGCGRLEWGVLNWNTPAIEFYESFGAKPQEEWTTYRLTGQALLDFAQG"
     gene            complement(5615..7009)
                     /gene="argH"
                     /locus_tag="VFF22_03475"
     CDS             complement(5615..7009)
                     /gene="argH"
                     /locus_tag="VFF22_03475"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HZX15913.1"
                     /translation="MSTEKTNQSWGGRFSEPVDAFVARFTASVEFDKRLYRHDIMGSI
                     AHATMLAKVGVLTDAERDAIVDGLTQIQSEIEAGQFDWRVDLEDVHMNIEARLTDRIG
                     VTGKKLHTGRSRNDQVATDIRLWLRDEIDLILAEITRLQQGLLEQAEREAETIMPGFT
                     HLQTAQPVTFGHHLLAWFEMLSRDYERLVDCRKRTNRMPLGSAALAGTTYPIQREVTA
                     QLLGFDAVGGNSLDGVSDRDFAIEFCAAASIAMMHLSRFSEELVLWTSAQFQFIDLPD
                     RFCTGSSIMPQKKNPDVPELVRGKTGRVFGALMGLLTLMKGQPLAYNKDNQEDKEPLF
                     DAADTLRDSLRAFADMIPAIKPKHAIMREAALRGFSTATDLADYLVRKGLPFRDCHEI
                     VGHAVKYGVESGKDLAEMSLEELRQFSEQIEQDVFAVLTLEGSVNARNHIGGTAPNQV
                     RAAVVRGRELLAAR"
BASE COUNT         1183 a         2051 c         2356 g         1435 t
ORIGIN      
        1 cgtcttagtg atggggtttt gcttttttca cgatccgtaa aggggacata cccgcaccat
       61 gaccagcgca ccttcgctca ccatcacccg tcttgacctg cagcgcctgg agcatctgct
      121 cgacagcctg gacgacttcg gtcccagtgc tatcgcgttg caggccgaac tggaccgcgc
      181 cgatgtggtt ggccatgacg aggtgccggc tggggtggtg accatgaatt cgcgggtgca
      241 ttgccgtgag gaaagcagcg gcaaggatta ccacctgacc ctggtttacc cgcaggacgc
      301 cggcggtgaa ggctgtgtgt cggtactggc gccggtcggc acggccctgc tggggctgtc
      361 cgtgggtcag catatcgact gggctgcgcc cggcggcaag gtgctcaagc tgacgctgct
      421 ggcggtggaa tatcagccgg aagcggcggg cgaatacagc cgcgccgagt gagcaggttg
      481 cgcaaagcct tctagagtag agctaagcca atctcggcat ggaggccgta tgtccagtct
      541 acctatgtac ttcccccaag gttttgacct ctctagggcg ctgctatgtg cgcaactggt
      601 cagctcggcc tatgaccaat acagccagtg gcaagcccag cagcgcccgc ggaggcccca
      661 ggatttcacc tggcagccgc ctacgctgcc cggctggggt ttttccgcgc cgatctggag
      721 cgtgctcagc gagctgcact ttctcaacga gtcggagccc ttcggcttcg ccgcgcgtga
      781 cccgcagggc gaggtctatc tagtgttccg tggcactgac acggtgcagg actggctgga
      841 tgatctggat gccgatcagc gcgcctaccc ctggcagcct ggcctgggca atgtgcatga
      901 cggctttctc aagctctaca cctcactgcg cgaccaggct ttgcaggccg tagatactca
      961 gcagcccaac gggccgctgt gggtgtgcgc gcacagcctg ggtggtgcgc tatccagttt
     1021 ggcagtgctg gatatgcgcg aacgctggcc ggacttgccg ctgcaacact acagctttgc
     1081 cagcccgcgc ctggccgccc cggactttgc cgcctactac aacgggctgc aggtgcccac
     1141 cttccgcgtg gtgaatgaca gcgatctggt gccggaggta ccgccggggg ttaccggcaa
     1201 gtggctgtat cagcacctgg gattggcggt gaccttcagc gccagctacg cctcggtggg
     1261 cgacaatcac agcctggccg gttgttacct gtacgccttg cagaaccctc aggcgccgat
     1321 gcgttgctga tcaggccttc tgcagcgcct gattcagcgc tgcttccagg cggctcttgt
     1381 agcgcaggta gtgcacgctc tgcatatgcc catcaccgag taccgccgac aggtcaaggt
     1441 cggtgatata gcaggggtag cgtgcgccat cgcggcgttg cgcgaggatg tgctgggcga
     1501 ccgcggcgaa caggtcgccg gcgtattcca gctcggaaaa ttcgcgatga ttacagtaca
     1561 gggtgacgtg cgatcgcttg tggtccgccg gctcgacgat ggcctgcacg tcatagaacg
     1621 gatggctgat cggcgatagc ggcggcggtc ggcgttccag gcgttgggcg cgcagcggcg
     1681 catccggcag tacctggtaa tacagcactt ccagcggctg atgcgccggc aggttatcca
     1741 gcggcagcag ggcattgcgc cggtacagca ctgattgcag aaagcgttgc agcggcgtca
     1801 gcaggctctg ctcatcacgg aacggcatct gctggcgcca cagcgcgttg tgctcgtcga
     1861 gcacgctgag ttcggccgtg gcgctggctt cgttcagccg gtagaacacc tgaatgcact
     1921 gcggccgccc cacgggcagg atcagcgcca ggtcttcacc ttccagggcg ctgcggtcca
     1981 gatgcagctc gctgtagccg ggcagctcct cgcccagatg gtcgatcagc gtgggcaggt
     2041 ctttcaggct ggtgtggctg acgtggccgg gcttcagttc taggacgtga tactgctgtt
     2101 tgatctgcag cagatagcgg ttggcctgtt cgctgctgaa gctgccgagg gtttcgcgga
     2161 atagttcttc gacccgctcg gcaatcgccg tggcgcggtt gcggcagaag cagcgcaccc
     2221 gcaggttggg cttgcgaccg ctggcgggca ggttgttgag gaagtctcgc aggcagtcga
     2281 gcagtgcatg cgggccgtca tagcgggtta ccagcagctc gttccagctg ttcagggtga
     2341 cctggtcgat ggtcagaatc aggttctcgc gcaccccgga atagcccagc gcatcggtgc
     2401 gctcggtggt catgtgcatg ttcatctggc tgaactgctt gagcggatcg atgccgacgt
     2461 tgaccaacag cagcacctcg ccgggcacgc tggcgctgag cagggcggct tcatcgaccg
     2521 ctggcaacgg cagcggcact acctgctgca agctgctgag caggttggac agctcgtatt
     2581 cggttaggtc gctgctgcct gggtgcagcg acagccgcgt gctgttgtcg attacgccat
     2641 tgcgctggca ccaggcgagc agttcgatca gctcgcgtga gcgcttgagc ggcgagaagt
     2701 cttccaggtc ctgagcgttg acgctgccac tgtagagcgc ccatagcgtt tcattgggct
     2761 cgtccgggct ggggctctgc gccagggtca gggtgtcttc ggcgaggtcc ggggcgatgc
     2821 ccgggttgat aaattcgatc ttcccggctt tgcgctcgaa ggcggcgtac aggcggcggc
     2881 cgaggatgcc cagatcgcgg ctgttgagcg agctaccggc atgttcgctg cgggcaaatt
     2941 ccgagagaaa gcggtagctg taggtcagct cgttaaccag catgcgccgt tcggcgctga
     3001 cctggcgcac cttccactgg ctgcggctgt cgagcatcgc caactggcgg ctgtcccagt
     3061 tccagtcgcg ggtcaggcgc tccagcagca ggcgttgcca gctcttgcgg ttgttgcgcg
     3121 gtgggcggct gagttttttg tcgaccttga ggtataggca gcggcggatc agctccaggc
     3181 gctcatgctc gccacgggct ttgaggtatt cctccaggcg ccggtagacc acgatatagg
     3241 ggtcgagctc atcgacgtcc aggcgattgg cgaacaccgc ctgcttgaaa cgcagcgaca
     3301 ggcattcgac cttcggatgt tcgctggcgt agacctcggt gagcagcagc ttgagcaccg
     3361 acttgtaagg cgactcgatg cccttgaaca gctgccacat gccggcgccg ataaattctt
     3421 ccggcggaat gcgcgccagg tgaccgaggt cgagcacgtc ctcggcgcgg ataaagcgct
     3481 tggccagcaa ggtgctggtg tagtgctggt agcgctgctc ttcatacacc ggcaccagcc
     3541 accacagcgg cgtgcgtccg ccgagccaga tggcggtgcg gtagaactcg tcgagcagca
     3601 ggtagtgctg ggtggtaccg cagtcgtctg aggtcagctg cgcctcgcgt tcgccctggg
     3661 tgaaacgtgc cgggtcgatc agaaagaaat gcgcctcggc gccgaaggtg gccgcccagc
     3721 tttccagcag cgcgcatttc ttgttcagtt catgcagctg ttgcgtgctc agatcgctgg
     3781 cgtggcacac ccacaggtcc atgtcgcttt gctcggcctg ggccacggta cccaggctgc
     3841 ccatcagaaa caggccatgg atcggcagcg cctgtttgcc gcgatagggt ttgtaggcga
     3901 acgagcgggt caggcgctgc acttcggaaa gcagttcgtc atccggctcg aagccgctga
     3961 ggccggccgg agtcagcccg gaaacgtagc ccggcagcag cggatgattg acgtggaaca
     4021 gcagcggtaa cagcttgagc accagctgct ggcgcgtcga cagcgcctgc atggcgcgtt
     4081 gcaggcggcc ctggttgagt ttgaggaagc gcgcacgcag ctgcgtcagt accttgcggt
     4141 cgataccgtc ttcaatgtcc gggcgaattt cctcggtacg actcatgctc aggctctgca
     4201 gggcggtgcg tgatcatgcc ttgccaggtg gctggcggac ggctgcggat acagggttat
     4261 cgcctgcgtc gcgggcagct tgagcaagcc gctgcaacgc aggctgggtt ggcttggaag
     4321 cggtttcgat ctcgctctgg ttggcgagag ttgcgggcgc ttctcaggcc gcttcggtca
     4381 cattgagaat ggtcagcagg gtttgcgcgt gttcggcggc atcaaggccc aagctggtca
     4441 gataaccacc gtctggctgg ttgatcagac ctttatcgtg caggcgttgg gcggcggcaa
     4501 tcgccgaggg ggtagcggtg tggtgaacct tgattccttc ctgagtggtg cccaggttga
     4561 acagtgcgag gatttccagt tcggcaatca gttcgggggt aaaggccata agtactccag
     4621 cttcttgttt tagtgagctg cggccggcgg cggcagcacc cgctgcggta aatcggctga
     4681 aacaacaggc agcctgcgaa ggcagtgtag tgaagcccca ggcagtctgc tgtgacctga
     4741 tgctgccaga agttggcgtg ttgtgggtta aatttttccg agctgtttac ggcgcctgtt
     4801 cgggcaactc aggcaaggcg cgcaggccct gctcgtagcg ggtgaggtcg aaggggcggt
     4861 cgtcgcgcag catgacgtcg atttcatcgg ccagcaccag ggccatcagc tcaatgatct
     4921 cttcgcgttc atagccgacc aggctcagct tgttcagcac cgcctggacg aaggccggct
     4981 cgccggcttc cagctggttt tcgatggcct ggatcagggt gtcctgggca aaatcttcgt
     5041 cttcttgctc gctcatgcgc gactccggta gatggtggtt tagccctggg caaaatccag
     5101 cagtgcttgg cctgtaaggc ggtaggtggt ccattcctct tgcggtttgg cgccgaagga
     5161 ctcatagaac tcgatagctg gcgtgttcca gttcagtact ccccattcca ggcggccgca
     5221 gcctttgctg acggcgagtt gcgccagatg ccttagtaac gccttgcctg cgccgatctt
     5281 gcgatgttca gggctgatgt aaaggtcttc cagatagatg ccgtgcttgc ccagccaggt
     5341 cgagtagctg aagaagtaca cggcatagcc aaccggcacg ccgtccagct cgcagatcag
     5401 cccgtgcgag gtgctgttgg cggcaaacaa gctgttctgg atgccggcgg cgtcggtctt
     5461 cacctcgtgc tcggcttttt cgtagatggc cagatcgata atgaagcgca ggatctgcgc
     5521 ggtgtcttcg atagtggcgg tgcggatttg caggggcata agggctccta ggttggccgg
     5581 ccggcgcgag tgaagcccac gccggggcgg agctttagcg tgcggcgagc agctcgcgac
     5641 cgcgcacaac cgccgcgcgc acctgattcg gtgcggtgcc gccgatatgg ttgcgagcat
     5701 tgaccgagcc ttccagggtc agcacggcaa acacgtcctg ttcgatctgc tcgctgaact
     5761 ggcgcagttc ttccaggctc atctcggcca ggtccttgcc agactcgacg ccgtacttca
     5821 ccgcatggcc gacgatctcg tggcaatcgc ggaacggtag gcccttgcgt accaggtagt
     5881 cggcgagatc cgtggcggtg gagaagccgc gcagcgccgc ttcacgcatg attgcgtgct
     5941 tgggcttgat cgccggaatc atatcggcaa aggcgcgcag cgaatcgcgc agggtgtcgg
     6001 cggcgtcaaa cagcggctct ttgtcttcct ggttgtcctt gttgtaggcc agcggctggc
     6061 ctttcatcag ggtcagcagg cccatcagcg caccgaacac gcggccggtc ttgccacgaa
     6121 ccagctcggg tacgtcgggg tttttctttt gcggcatgat cgagctgcct gtgcagaagc
     6181 gatccggcag gtcgataaac tggaactgcg cgctggtcca gagcaccagc tcttcggaga
     6241 aacgcgacag gtgcatcatg gcaatcgagg cggcggcgca gaattcgatg gcgaagtcgc
     6301 gatccgatac gccatccagc gagttgccgc caacggcgtc aaaaccgagc aactgcgcgg
     6361 tgacttcgcg ctgaatcggg tacgtggtgc cggccagtgc cgccgagccc aggggcatgc
     6421 ggttggtacg cttgcggcaa tccaccaggc gctcgtaatc gcggctaagc atttcgaacc
     6481 aggccagcag gtggtggccg aaggtcaccg gctgagcggt ttgcagatgg gtgaagcccg
     6541 gcatgatggt ctcggcttcg cgttcggcct gttccagcag gccctgctgc aggcgggtga
     6601 tttcggcgag gatcaggtcg atctcgtcgc gcagccacag gcggatatcg gtggcgacct
     6661 ggtcgttgcg gctacgcccg gtgtgcagct tcttgccggt gacgccgatg cggtcggtca
     6721 ggcgcgcttc gatgttcatg tgcacgtctt ccagatcgac gcgccagtcg aactggccgg
     6781 cttcgatctc gctctgaatc tgcgtcagac cgtcgacgat ggcatcgcgc tcggcgtcag
     6841 tcagtacgcc gaccttggcc agcatggtgg cgtgggcgat ggagcccatg atgtcgtggc
     6901 gatacaggcg cttgtcgaat tcgacggagg cggtaaagcg ggcaacaaag gcatcgactg
     6961 gctcgctgaa gcggccgccc caggattggt tggttttttc agtgctcatg ggcttcgctc
     7021 ggctg
//
LOCUS       DASRUM010000046         7426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2195392, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000046 DASRUM010000000
VERSION     DASRUM010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7426
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2195392"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VFF22_03480"
     CDS             complement(<1..757)
                     /locus_tag="VFF22_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZX15914.1"
                     /translation="MHPLHSLHNLHRPLGATELLVSPLGLGTVKLGRDQGVKYPNGFT
                     IPGDAAARELLAQAHDLGINLIDTAPAYGTSETRLGPLLRGQRQQWVIVSKVGEEFDN
                     GQSHFDFSPAHTRFSVERSLKRLETDFIDLVLVHSDGNDVAILRDSGVYETLAELKRE
                     GKIRGFGLSGKTVEGGLLALEHGDCAMVTYNLNEQGEQAVLDYAELHGKGILIKKALA
                     SGHACLAPGVDPVRASFELIFGHPGACSAIIGTI"
     gene            complement(757..1929)
                     /locus_tag="VFF22_03485"
     CDS             complement(757..1929)
                     /locus_tag="VFF22_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZX15915.1"
                     /translation="MSQALSTDVLIVGGGIAGLWLNARLRRQGFATVLVENARLGGGQ
                     SVKSQGIIHGGAKYALHGALTGASEAIADMPRRWREALAGSGELDLAGVRLLSDAHYL
                     WSPGSLAGNITSFFASKAVRGRVDQVKGEQLPPALQHPKFKGKVYRLAELVLDVPSLI
                     SRLSELAGDGLLAAERIEPLQENGELIGLRIDGREIRAQRIILSAGRGNAELLSSLGL
                     SQPAQQLRPLHMALVKGPTLKPLYAHCLGGGPKPRVTITTHPAADGQWVWYLGGDLAE
                     ADGVARDETAQIQAAKKELSELLPWVDLSAAQWATLRVERAEPAQSGLVRPDNAFLHE
                     QDKLLVGWPTKLALAPDFADRVLAALARDGIQPAQHAPLPALPRPTVAPAAWEELF"
     gene            complement(2006..2338)
                     /locus_tag="VFF22_03490"
     CDS             complement(2006..2338)
                     /locus_tag="VFF22_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HZX15916.1"
                     /translation="MGGYLYLAIAITAEVIATTSMKALDGFNKPLPLLLVVVGYAISF
                     WMLSLVVKTIPVGVAYAVWAGLGIVLVSIAAVFLYQQRLDLPAMLGMGLIVAGVVVIQ
                     LFSQSGGH"
     gene            2426..3310
                     /locus_tag="VFF22_03495"
     CDS             2426..3310
                     /locus_tag="VFF22_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX15917.1"
                     /translation="MQWSLEQIRLFVSVADGQSFSAVGRQMQRAQSAVSNAIALLEAD
                     LGVRLFERSSGRQPRLTEAGAALLEEAREVLRQCQRLDGRALGLVRGEEVRLRLAQDE
                     AMPYQPVLDSLEALAQQFPLLEVQLASGAQGDVARKLLEHRADLGLLFHHEQMPDALE
                     RQRLGTIEMVTVCGAGHALAGAGHVDRRELARHRQLLMAPQDSHYPGGEQLSPSVWRT
                     DSFYAMAELLMRNLGWAWLPRHVVQYPTYQSQLVELSSDWTPPPLVVELVCRRDEALG
                     PAALWLAECFAQHLQAIG"
     gene            3363..4655
                     /gene="waaA"
                     /locus_tag="VFF22_03500"
     CDS             3363..4655
                     /gene="waaA"
                     /locus_tag="VFF22_03500"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HZX15918.1"
                     /translation="MNRSLYTLLFHLGLPLVAGRLAWRAWQAPAYAKRIGERFALGLP
                     PLKPGGIWLHAVSVGESIAAAPLVRELLVRYPQLPITITCMTPTGSERIQAMFGGNEY
                     AGRVQHCYLPYDLPWAAARFLDQVQPKLAVIMETELWPNHIHQCAKRGIPVALANGRL
                     SERSARGYARFANLTAPMLAELDLLAVQTALEAERFRNLGARPECVEVTGSIKFDLTI
                     DPELLTRAAALREQWQAESRPVWIAASTHAGEDEIVLAGHRELLKQKPDALLILVPRH
                     PERFNAVSELCQRQGFSTRRRSTGEALLASDQVLLGDTMGELLFLYALADIAFVGGSL
                     VANGGHNLLEPAALGKPVLSGPHLFNFLEIAAQLRAAGALSEVADANSLAQQVAQLWR
                     EPETAQAMRAAGQAVMQANQGALARLLAGLGRLLAGKP"
     gene            complement(4726..6171)
                     /locus_tag="VFF22_03505"
     CDS             complement(4726..6171)
                     /locus_tag="VFF22_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HZX15919.1"
                     /translation="MLRRLSLALAVAAASNGMAWAVEQAPLSTKTDLVTVYQEAAANN
                     ADLAAARADYEARREVVPQARAGLLPNLSAGANLSDTRTQVDSPSITSSRSGTVYQAN
                     LSQPLFRADRWFQLQAAEATNEQAALELSATEQDLILQSAETYFAVLRAQDNLASTKA
                     EEAAFKRQLDQANERFDVGLSDKTDVLEAQAGFDTARANRIIAQQQVEDAFEALITLT
                     HRDFAALEGIEHSLPILAPTPNDAKAWVDTAAAQNLNLQASNYAVEAAEETLRQRKSG
                     HAPTLDAVASYQKGDNDSLGFSNSGTPGQARFSGDVEQRSIGLQLNIPLYSGGLTSSQ
                     VRESYQRLNQTEQLRESLRRQVVQNTRNLHRAVNTDVETVQARKQSIISNQSALEATE
                     IGYQVGTRNIVDVLDAQRQLYSSVRNYNNARYDYILNNLRLKQAAGTLSPDDLAALEQ
                     YLKSDYNPDEDFLPPDLAKAAEAQLRGNPQY"
     regulatory      6393..6499
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6595..>7426
                     /locus_tag="VFF22_03510"
     CDS             6595..>7426
                     /locus_tag="VFF22_03510"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HZX15920.1"
                     /translation="MSTQEKNLNPILSESAQVDQQSIQPFTRSQKIYVQGSRPDILVP
                     MREISLDVTPTDFGGEINAPVVVYDTSGPYTDPNVIIDVRKGLADVRSPWIEARGDTE
                     RLPGLSSNFGQQRLADAELTKLRFAHVNNPRRAKAGANVSQMHYARKGIITAEMEYVA
                     IRENMKLEVARSAGLLDQQHAGHSFGASVPKIITPEFVRDEIARGRAIIPANINHTEL
                     EPMIIGRNFLVKINGNIGNSALGSSIEEEVAKLTWGIRWGSDTVMDLSTGKHIHETRE
                     W"
BASE COUNT         1264 a         2232 c         2490 g         1440 t
ORIGIN      
        1 tgatggtgcc gatgatcgcg ctgcaggcgc ccgggtggcc gaagatcagc tcgaaactgg
       61 cgcgcaccgg gtcgacaccc ggcgccaggc aggcgtgacc gctggccagg gccttcttga
      121 tcaggatgcc tttgccgtgc aactcggcat agtcgagtac tgcctgttcg ccttgctcat
      181 tgaggttata ggtgaccatc gcacagtctc cgtgctcaag ggccagcagg ccgccctcaa
      241 cggttttgcc ggagaggcca aaaccgcgaa tcttgccctc gcgtttaagc tcggccaggg
      301 tttcgtatac gccgctgtca cggaggatgg ccacgtcgtt gccgtcggaa tgcaccagca
      361 ccaggtcgat aaaatcggtt tccagacgtt tgaggctgcg ctccaccgag aaacgggtgt
      421 gcgccgggct gaagtcgaag tgcgactggc cgttgtcgaa ctcttcaccg accttgctga
      481 caatcaccca ctgctggcgc tggccgcgta acaacgggcc gaggcgagtt tcgctggtgc
      541 cgtaggccgg cgctgtgtcg atcaggttga tgcccaggtc atgggcctgg gccagtagtt
      601 cgcgggcggc ggcgtcgccg gggatggtga aaccgttggg gtatttcacc ccctgatcac
      661 ggcccagctt gaccgtgccc aggcccagcg gcgaaaccag cagctccgtg gcgcccaatg
      721 ggcgatgcag attatgcagg ctgtgcagtg gatgcatcag aacagttcct cccaggcggc
      781 gggggcgacg gtcgggcgcg gcagtgcagg cagtggtgcg tgttgcgccg gctgaatgcc
      841 gtcacgggcc agcgccgcca gcacgcggtc ggcaaaatcc ggggccagag ccagtttagt
      901 cggccagccc accagcagct tgtcctgctc atgcaggaag gcattgtccg ggcgtaccaa
      961 gccggattgc gccggttcag cgcgctccac ccgcagcgta gcccattgcg cggcggacag
     1021 atcgacccag ggcagcagct cgctcagctc ttttttcgcg gcctggatct gcgccgtttc
     1081 gtcacgggcc acgccatcgg cttcggccag atctccaccc aggtaccaga cccactggcc
     1141 gtcggcagcc gggtgggtgg tgatggttac gcgcggcttc ggcccgccgc ctaggcagtg
     1201 ggcgtacagt ggtttcagcg tcgggccttt gaccagggcc atatgcagcg ggcgcagttg
     1261 ctgggccggc tggctgaggc cgaggctgct gagcagttcg gcattgcccc ggccggcgct
     1321 gaggatgatg cgctgtgcgc gaatctcccg gccgtcgatg cgcaggccta tcagctcgcc
     1381 attttcctgc agcggctcaa tgcgctcggc ggccagcaaa ccatcgccgg ccagctcact
     1441 caggcggctg atcaggcttg gcacatccag tactagttcg gccaggcggt agactttgcc
     1501 cttgaatttc gggtgttgca acgccggtgg cagttgctcg cccttgacct gatcgacccg
     1561 gccgcgcact gccttgctgg caaagaaact ggtgatattg ccggccagac tgcccggcga
     1621 ccacaggtag tgggcatcgg agagcaggcg cactccagcc agatccagct caccgctgcc
     1681 ggccaaagct tcgcgccagc gccgcggcat atcggcgatg gcttcggagg caccggtcag
     1741 cgcgccgtgc agggcgtatt tggcgccgcc atggatgatg ccctgggatt tcacactctg
     1801 cccgccgccg aggcgggcat tttccaccag cacggtggca aagccctgac ggcgcaggcg
     1861 cgcattcagc cagaggccgg cgataccgcc gcccacgatc aaaacatcag tgcttaacgc
     1921 ctgggacatg catgtgtctc accgggaaaa caggcgcgca gtataacccg ctcgcgctag
     1981 cgcgcctccc tggcccagaa ccggcttaat gcccgccgct ttgcgagaac aactgaatca
     2041 ctacgacgcc cgcgacgatc aggcccatgc cgagcatggc cggcaggtcc aggcgctgct
     2101 ggtagagaaa caccgcggcg atgctcacca ggacgatgcc caggcccgcc cacaccgcgt
     2161 aagcgacgcc aaccgggatg gtcttcacca ccaggctgag catccagaac gagatggcat
     2221 agccgaccac aaccagcagc aggggcaggg gtttgttgaa accatccagg gccttcatcg
     2281 aggtagtggc gatgacttcg gcagtgatgg caatagcgag gtagagatag ccgcccatga
     2341 tgtggcctcc ttggtggtct ggcggccatt ctagtgatct ggcggatggg ataaagtgat
     2401 tacctatctg aaatggagat gggctatgca gtggagcctg gagcagattc gcctgtttgt
     2461 cagcgtcgcc gatggtcagt cgttctctgc tgtgggccgg caaatgcagc gcgcacaatc
     2521 agcggtgagc aatgcgattg ccttgctgga ggcggatctc ggcgtgcgtc tgttcgagcg
     2581 cagcagcggc cgtcagccac gcctgaccga ggccggtgcg gccctgctgg aagaggcgcg
     2641 tgaggtgctg cgccagtgtc agcgtctgga tgggcgtgcg ctgggtctgg tgcgcggtga
     2701 agaggtgcgc ctgcgattgg cccaggatga ggccatgcct tatcagccgg tactcgacag
     2761 cctggaggcg ctggctcagc aatttccgct gctggaggta cagttggcca gcggcgccca
     2821 gggcgatgtg gcgcgcaagc tgctggagca tcgcgccgac ctcggtctgt tgtttcacca
     2881 cgagcaaatg cccgacgccc tggaacggca gcgcctgggc actatcgaga tggttaccgt
     2941 gtgcggggca gggcatgcgt tggccggagc cgggcatgtc gaccggcgtg aactggcgcg
     3001 gcatcggcaa ctgctgatgg cgccgcagga cagccattat ccgggcggcg aacagctcag
     3061 cccgtcggtc tggcgtaccg acagcttcta cgccatggcc gagctgctga tgcgcaacct
     3121 cggctgggcc tggctgccgc gccatgtggt gcagtacccg acctatcaaa gccagttggt
     3181 tgagctgagc agcgattgga ccccgccgcc gctggtggtg gagctggtgt gccgtcgcga
     3241 tgaggcgctg gggcccgcgg cgttgtggct ggccgagtgc tttgcccagc atttgcaggc
     3301 gattggctag cacgccggcg cgttactcga cgatattggc caaagtgcat aagctgcgcg
     3361 ccatgaatag aagcctctac acactgctgt ttcatcttgg tctgccgctg gtggccgggc
     3421 gcctggcctg gcgtgcctgg caggcaccgg cctatgccaa acgtatcggc gagcgctttg
     3481 ctcttggttt gccgccgcta aagcccggcg gtatctggct gcatgcggtg tcggtaggcg
     3541 agagcatcgc cgccgcgccg ctggtgcgtg agctgctggt gcgctacccg cagttgccga
     3601 tcaccatcac ctgcatgacg ccgaccggtt cggagcgcat ccaggcgatg ttcggcggca
     3661 atgagtacgc cgggcgggtg cagcattgct acctgccgta tgacctgccc tgggcggcgg
     3721 cgcgttttct cgatcaggtg cagcccaagc tggcggtgat tatggaaacc gagctgtggc
     3781 ccaaccatat ccaccagtgt gccaagcgtg gcatacccgt ggcgctggcc aacgggcggc
     3841 tgtccgagcg ctcggcccgt ggctatgcgc ggtttgccaa cctcactgcg ccgatgctgg
     3901 ccgagctgga tttgctggcg gtgcaaacgg cgctggaagc cgagcgcttt cgcaacttgg
     3961 gcgcgcgacc tgagtgtgtt gaggttaccg gctcgatcaa gtttgatcta acgattgatc
     4021 cggagctgct gacccgcgca gcggccttgc gtgagcaatg gcaggccgag tcgcgcccgg
     4081 tgtggatcgc cgcaagcacc catgctggtg aagacgaaat cgttctcgct ggccaccgcg
     4141 agctgctcaa gcagaagcct gatgccctgc tgattctggt gccgcgccac cctgagcgct
     4201 tcaacgcagt atctgagttg tgccaacggc aaggtttcag cacacggcgc cgctccactg
     4261 gcgaagcgct gctggcgagc gatcaggtac tgcttggcga caccatgggc gagctgctgt
     4321 ttctctatgc cctggccgat atcgcctttg tcggcggcag cctggtggcc aatggcgggc
     4381 ataacctgct ggagccggcg gcgctgggca agccggtgct gagcggcccg cacctgttca
     4441 actttctgga gattgccgcg cagttgcgcg cggcgggcgc cttgagcgag gtggcggatg
     4501 cgaacagtct ggcccaacaa gtcgcgcaac tatggcgtga gcccgaaaca gcacaggcca
     4561 tgcgggctgc tgggcaggcg gtgatgcagg ccaatcaggg cgcgctggcg cgtttgctgg
     4621 ctggcctggg ccgattgtta gcgggcaagc cttaacaggc tcctgcaaat ggatgcgcca
     4681 taaaaaagcc cgctgaattc agcgggcttt tttgttttgc tcaactcagt actgcgggtt
     4741 gccacgcagc tgggcttcgg cggctttggc caggtccggg gggaggaagt cttcatccgg
     4801 gttgtagtcg gatttcagat actgctcgag ggcagccagg tcgtccgggc tgagggtgcc
     4861 ggcggcctgc ttcaagcgca ggttgttgag gatgtagtcg taacgcgcgt tgttgtagtt
     4921 acgcaccgag ctgtacagct ggcgctgggc gtcgagcacg tcaacgatgt tgcgggtgcc
     4981 gacctggtag ccgatttcgg tggcttccag ggcgctctgg ttggagatga ttgactgctt
     5041 gcgcgcctgt acggtttcca cgtcggtatt cactgcgcgg tgtaggttgc gggtgttctg
     5101 caccacctga cggcgcaggc tttcacgcag ctgctcggtc tggttcaggc gctggtagga
     5161 ctcgcgcacc tgggagctgg tcaggccgcc gctgtacagc gggatattca gttgcaggcc
     5221 aatcgagcgt tgctcgacat cgccgctgaa ccgcgcctgg ccgggcgtgc ccgagttgct
     5281 gaagccgagg ctgtcgttgt cgcccttctg gtagctggca accgcatcca gggtcggtgc
     5341 gtggccagat ttgcgctggc gcaaggtttc ttcggcggct tcaacggcgt agttgctggc
     5401 ttgcagatta aggttctgtg cggcggcagt gtcgacccag gccttggcgt cattcggcgt
     5461 cggcgcgagg atcggcaggc tgtgctcgat gccttccagt gcggcgaagt cgcggtgggt
     5521 cagggtgatc agagcctcga aggcatcttc cacttgctgc tgggcgatga tgcggttggc
     5581 gcgagcggtg tcgaagccgg cctgggcttc gagcacatcg gtcttgtccg agaggccgac
     5641 atcaaagcgc tcgttggctt gatccagctg acgcttgaac gctgcctctt ccgccttggt
     5701 cgaggctagg ttgtcctggg cgcgcagtac cgcgaagtag gtttcggcgc tttgcagaat
     5761 caggtcctgc tcggtggccg acagttccag cgcagcttgc tcgttggtcg cttcggcggc
     5821 ctgcagctgg aaccagcggt cagcgcggaa cagcggctgg ctgagattgg cctggtacac
     5881 cgtgccgctg cgcgagctgg tgatggacgg cgaatcgacc tgggtgcggg tgtcgctcag
     5941 gttggcgccg gcagacaggt tgggcagcag gccggcgcgg gcctggggga ctacttcgcg
     6001 gcgtgcttca taatcagcac gggcggcggc caggtcggca ttgttggcgg cggcttcctg
     6061 gtaaaccgtg accaggtcag ttttggtaga cagcggggct tgttcaacag cccaggccat
     6121 tccgttggac gcggcggcca cagcgagagc cagagagagt ctgcgcaaca tgggtgtgat
     6181 cctgattttg gcggtaatcg gcaaggctat aggggcgctg cctagagggt caagatttgt
     6241 cttgggccgg gtgagtgtag attgcaacaa accatggcgt cttctgcgtg tcgggttatt
     6301 tgtaatgacc gctttcgtca ttttccgacg cgtgggtcat aagaaattga ctcccgagtt
     6361 ggttttattg gctggggttg attagactgg ccgcgttctt gtcggggtgc cccaattgcg
     6421 gggctgagat tggcagctgc cggatcccgt tgaacctgat caggttaagg cctgcgtagg
     6481 gaacaagatg tgctcgcctt tcccctggcg agcctctggc accgcccctg gtgcgccttt
     6541 cgccgctcat ttatctccag gttcgctccg acatatagcc ctggagagcc gccgatgagc
     6601 acacaagaaa aaaacctcaa tccgatcctg agtgagtcag cacaggtcga ccaacagtcg
     6661 atccaaccct tcacccgttc gcagaaaatc tatgtgcagg gctcgcgccc ggacatcttg
     6721 gtgccgatgc gcgagatcag cctggacgtc acccctaccg acttcggcgg cgagatcaac
     6781 gcgccggtgg tggtctacga cacctccggc ccgtacaccg acccgaatgt gatcatcgac
     6841 gtgcgcaagg gcctggccga tgtgcgctcg ccatggatcg aagcgcgtgg tgacaccgag
     6901 cgcctgcctg gcctcagctc caatttcggc cagcagcgcc tcgccgatgc cgagctgacc
     6961 aagctgcgtt tcgcccacgt caacaacccg cgccgcgcca aggccggggc caatgtcagc
     7021 cagatgcact acgcgcgcaa aggcatcatc accgccgaga tggaatacgt cgctatccgc
     7081 gaaaacatga agctggaagt ggcgcgttcc gccggcctgc ttgatcagca gcacgccggt
     7141 cacagcttcg gtgccagcgt gccgaagatc atcacccccg aattcgtgcg tgacgagatt
     7201 gctcgcggcc gcgcgatcat cccggccaac atcaaccaca ccgagctgga gccgatgatc
     7261 atcggccgca acttcctggt gaagatcaac ggcaatatcg gcaactcggc gctgggttct
     7321 tcaattgaag aagaagtcgc caagctgacc tggggcatcc gctggggttc ggacacggtc
     7381 atggacctgt ccaccggcaa gcacattcac gaaactcgcg agtgga
//
LOCUS       DASRUM010000047         4411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1700967, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000047 DASRUM010000000
VERSION     DASRUM010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700967"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..1144
                     /locus_tag="VFF22_03515"
     CDS             350..1144
                     /locus_tag="VFF22_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15921.1"
                     /translation="MTRLSLLLAASLFTLNASALELAKYPQVFNAGKGISVTVAPSSD
                     GKQALIQVSGINHPLDEVVLLSTINERGNDERDYTTTLDGSAYNMLIKRQGWGGEQYQ
                     LYLPSTDGFELGFDETKSKETKPANLLALYEQQKKKGVQDKLSSFDREKRVADFTQQL
                     QQIDQDASKACNTPLVTKVEWSAIDDDKLKRLSVPSFCGEVVSQLATLCQNGDEQKAA
                     AKGLKTVHCSFAQQLKLRENEANLTFTTHEEAPNQGDFINAFLRNR"
     gene            1246..2238
                     /locus_tag="VFF22_03520"
     CDS             1246..2238
                     /locus_tag="VFF22_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZX15922.1"
                     /translation="MLHAHLTTLHVVSLILEIFAAQPELTEQLLAGSGIAAADLDCAD
                     TRITRTQELQVCANALQLRSDLGLELGRRMHVSAYGMVGYAALSSATFGDAWRLMLQY
                     PALLGTYFKLDMHLEGELAWVSASDYQHSPALEVFNLEMCLASLKLISDDLLGQPLPL
                     SAAQFRYGEPDYSARYPHSFACPLQFDATRNAFAFPASWLQQPLPLADAVTHREMLER
                     CRKQNSEFNSRQAWLARIRQLLAERLTAPPGLEQLAQQMHCSPRTLRRHLQELGTHYQ
                     ELLDELRFERAKTLLGQEDWPICRIAEYLGFSETASFRHAFQRWSGVPPSRFRA"
     gene            2507..3541
                     /locus_tag="VFF22_03525"
     CDS             2507..3541
                     /locus_tag="VFF22_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010168350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HZX15923.1"
                     /translation="MLTFYSDDHHLHHGSCELIDGQLMPCFEKPQRADHILARVKDRQ
                     IGEVRPPQDFGLAPIERIHSGAYLDFFKGAWDRWLAQNGSGDLLPFTWPARTLRQVIP
                     RDLHGQLGYYSFDGGAPITAGTWQAAYSAAQVALSGQQAIANGAHSAFALCRPPGHHA
                     AGELMGGYCYLNNAAIAAQAFLDQGYKRVAILDVDYHHGNGTQDIFYTRSDVLFASIH
                     GDPADEFPFFLGYSDERGEGAGEGCNFNYPLAMGSPWAVWSAALDEACQRIAEYGAEV
                     IVVSLGVDTFKDDPISQFKLDSPDYLAMGKRIAALGLPTLFVMEGGYAVEAIGVNAVN
                     VLEGFEAATA"
     gene            3681..>4411
                     /locus_tag="VFF22_03530"
     CDS             3681..>4411
                     /locus_tag="VFF22_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZX15924.1"
                     /translation="MKTIKHLLAVAICGATLLASAAQAQTPEKQRELRVYNWADYMLP
                     TVPKEFQEKSGIKLTWDVFDTNEALEAKLLTGNSGYDLVVPTNTFLDTQIKAGVFQKL
                     DKSKLPNWQHLDPGLLQLMTANDPGNQYAVPYMYGTVLIGFNPDKVKAVLGADAPVDS
                     WDLIFKEENISKLKQCGVAMLDSPGDILPIALHYLGLDPNSSKPEDYEKASALMLKIR
                     PHVAYFHSAKYMTDIANGDICVAIG"
BASE COUNT          924 a         1440 c         1269 g          778 t
ORIGIN      
        1 caatcggact tggagcccat gatcacgcca accagtgcgc tcatcgtcgt gcctcttctt
       61 catcaagtgc ccgtaggcgc tgcagaaact aacaagccac gctggaaacc aggcgtggct
      121 tgttagggaa tgaattatgg cagtgcggac cgccaaaggc cgcgcagtat accgtaaagc
      181 ctgggctggc gtgcaccccg gcgcgccgtt tgtcgcggga cgcccaccgc gatgccaggc
      241 gacacgccaa cgcggttccg ggccgacgtc acgagcagac ctggcgcaca aatcgcagcc
      301 tgcggcctgg ctatactggg cgttcttttc agctaccaag gacaacacca tgacaaggct
      361 gtcactgcta ctcgccgctt cactgttcac cctcaacgcc agcgccctcg aactggccaa
      421 atacccacaa gtgttcaatg ccggcaaagg catcagcgtg accgtcgcgc ccagcagcga
      481 cggcaaacag gcgctgatcc aggttagcgg catcaatcac ccgctggatg aagtggtgct
      541 gctgagcact attaacgagc gcggcaacga tgagcgcgat tacaccacca ccctcgatgg
      601 cagcgcctac aacatgctga tcaagcgcca gggctggggc ggcgagcagt atcagctgta
      661 tctgccgagc accgacggtt tcgagctggg cttcgatgaa accaagagca aagagaccaa
      721 accggccaac ctgctggcac tttatgaaca gcagaagaaa aagggcgttc aggacaagct
      781 gagcagcttt gaccgggaaa agcgtgtggc cgacttcacc cagcagctac agcagatcga
      841 ccaggacgcc tcgaaggcct gcaacacccc gctggtcacg aaggttgagt ggagcgccat
      901 cgacgacgac aagctcaagc gcctcagcgt gccgagtttc tgcggtgaag tggtcagcca
      961 gctggccact ctgtgccaga acggcgatga gcaaaaggct gcagccaagg gcctgaaaac
     1021 cgtgcattgc agctttgcgc agcagctcaa gctgcgcgaa aacgaggcga acctgacctt
     1081 caccacccat gaggaagccc ccaatcaggg cgactttatc aacgccttcc tacgtaatcg
     1141 ctaaatctgc ctggaccgca gcggcctgga tcacgcccgg ccgctacggg gccgcggcat
     1201 agttcttgcg tcactcgggc attctgaact gttgcggttg ccccgatgtt gcacgcccac
     1261 ctgaccaccc tgcatgtggt ttcactgatc ctggaaatct ttgccgcgca acccgaactg
     1321 accgagcagc tattggccgg cagcggcatt gctgcggctg atctggactg tgccgacacg
     1381 cgtatcaccc gtacccagga gttgcaggtc tgcgccaatg ccctgcagct acgcagtgat
     1441 ctgggcctgg agctgggccg gcgcatgcat gtgtcggctt atggcatggt cggttacgcc
     1501 gcactctcca gtgccacctt tggtgacgcc tggcggctga tgctgcagta cccggcgctg
     1561 ctcggcacct acttcaaact ggatatgcac ctcgaaggcg agctggcctg ggtcagcgcc
     1621 agcgactacc agcacagccc tgcgctggag gtgttcaacc tggaaatgtg cctggcctca
     1681 ctcaagctga tcagcgatga cctgctcggc cagcctctgc cgctcagcgc cgcgcagttc
     1741 cgctacggcg aacccgacta cagcgcgcgc tacccgcaca gcttcgcctg cccgctgcag
     1801 tttgatgcca cgcgcaatgc ttttgccttc cctgccagct ggctgcaaca accactgccg
     1861 ttggccgatg cggtgaccca tcgggaaatg ctcgaacgct gccgcaagca aaatagcgag
     1921 ttcaacagcc gccaggcctg gctcgcgcgc atccgccagc tactggccga acgactgacc
     1981 gcgcctcctg gcctggaaca gctggcgcag cagatgcact gctcaccgcg caccctgcgt
     2041 cgccatctgc aggaacttgg cacgcactat caggaactac tcgacgaact gcgcttcgag
     2101 cgggcgaaaa ccctgctcgg ccaagaagac tggcctatct gccggatcgc cgaatacctc
     2161 ggttttagcg aaacggccag ttttcgccat gccttccagc gctggagcgg cgtaccgcca
     2221 agccgttttc gcgcctgagc acatggtgct gtgacgtagg gtggatgacg ctttatccat
     2281 ccaccaagcc atggcaaagc tggtggatga aaagaacgcc atccacccta caggctgtgc
     2341 gaacctattg gttttgcgcc gcattcatgg tgcgcgcggc acatcccagg gcgttgcggt
     2401 cgctaaattt ggccacttcg atcccctttt ggccgttgtc agcgttctcc atccgggtgt
     2461 gcaggcgaac aatgcaggca caacaacacg cctggagccg cttcacatgc tcacctttta
     2521 cagcgacgac catcacctgc accacggcag ctgcgagctg atcgacggcc agctgatgcc
     2581 ttgtttcgag aagccacaac gcgccgacca tatccttgcg cgcgtcaagg atcgccaaat
     2641 cggcgaagtg cgcccgccgc aggactttgg cctggcgccg attgagcgca ttcacagcgg
     2701 cgcttatctg gacttcttca agggtgcctg ggatcgctgg ctggcgcaga acggcagcgg
     2761 cgacctgctg cccttcacct ggccggcgcg caccctgcgc caggtcatcc ccagggattt
     2821 gcacggccaa ctcggttact acagcttcga cggcggcgcg ccgatcaccg caggcacctg
     2881 gcaagcggct tacagcgccg cgcaggtggc gctaagtggc cagcaagcca ttgccaacgg
     2941 tgcccatagc gccttcgccc tgtgccgccc gcctggccat cacgccgccg gtgagttgat
     3001 gggcggctac tgctacctga acaacgcggc catcgccgcc caggccttcc tcgatcaggg
     3061 ctataaacga gtggcgatcc tcgatgtcga ctaccaccac ggcaacggca cccaggacat
     3121 tttctataca cggagtgatg tgctgttcgc ctcgatccac ggcgacccgg ccgacgagtt
     3181 tccgttcttc ctcggctata gcgatgaacg tggcgaaggg gccggcgaag gctgcaattt
     3241 caactacccg ctggccatgg gcagcccctg ggcagtgtgg agcgccgcgc tggatgaagc
     3301 ctgccagcgc atcgccgaat acggcgccga ggtgatcgtg gtgtcactgg gcgtcgacac
     3361 ctttaaggac gacccgattt cccagttcaa gctcgacagc ccggattacc tagccatggg
     3421 caagcgcatc gccgccctcg gcctgccgac cctgtttgtg atggaaggcg gctatgcggt
     3481 ggaggccatc ggcgtcaacg cagtcaacgt gctggaaggg ttcgaagccg ccactgcgta
     3541 gggtggaccg gggcgcgcag ctgacgctgt tttcgtccac cgcgagcgat ccagataact
     3601 gaaggtttgc caaatgcctg gtggatggat aaagcgccat ccaccctaca catatcacaa
     3661 tgtctaaagg gtgaagaacg atgaaaacaa tcaaacacct gctcgccgtc gccatctgcg
     3721 gcgcaaccct gctggccagt gcggcccagg cgcaaacccc agagaagcag cgcgaactgc
     3781 gcgtgtacaa ctgggccgac tatatgctgc cgacggtgcc taaagagttt caggaaaaaa
     3841 gcggcatcaa gctgacctgg gatgtgttcg acaccaacga ggcgctggag gccaagctgc
     3901 ttaccggcaa ctccggctac gacctggtgg tgccgaccaa caccttcctc gacacccaga
     3961 tcaaggccgg cgtattccag aagctggaca agagcaagct gccaaattgg caacacctcg
     4021 accccggcct gctgcagctg atgaccgcca acgaccccgg caaccagtac gccgtgccct
     4081 acatgtacgg caccgtgctg atcggcttca acccggacaa ggtcaaggcc gtgcttggcg
     4141 ccgatgcgcc ggtggacagc tgggacctga tcttcaagga agagaacatc agcaagctca
     4201 agcagtgcgg cgtggccatg ctggattcac ccggcgatat cctgccgatc gccctgcatt
     4261 acctgggcct cgaccccaac agcagcaagc ctgaggacta cgaaaaggcc agcgcgctga
     4321 tgctgaagat tcgccctcac gtcgcctact tccactccgc caagtacatg accgatatcg
     4381 ccaacggcga catctgcgtg gccatcggct a
//
LOCUS       DASRUM010000048         4001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1533317, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000048 DASRUM010000000
VERSION     DASRUM010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533317"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..2936
                     /gene="uvrA"
                     /locus_tag="VFF22_03535"
     CDS             102..2936
                     /gene="uvrA"
                     /locus_tag="VFF22_03535"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003186003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZX15925.1"
                     /translation="MDKILIRGARTHNLKNIDLTLPRDKLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSMMEKPDVDTIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIYDYLRLLYARAGIPRCPDHDLPLEAQTVSQMVDQVLALPEGSKLMLLAPVIRERKG
                     EHLAIFDELRAQGFVRVRVNGKIYELDELPKLDKQKKHSIDAVVDRFKARGDLQQRLA
                     ESFETALKLADGLALIAPMEGEEGEEIIFSARFACPVCGHSISELEPKLFSFNNPAGA
                     CPTCDGLGVKQFFDTKRLVNGELTLAEGAIRGWDRRNVYYFQMLGSLAAHYGFSLEVP
                     FDELSAEHQKCILFGSGTQNVDFKYLNDRGDIVKRSHPFEGIVPNLERRYRETESTSV
                     REELAKFLSTQACQDCRGTRLRREARHVWVGEKTLPAVSGMPIGDATEYFGSLALPGR
                     RGEIAEKILKEIRERLQFLVNVGLDYLTLDRSADTLSGGEAQRIRLASQIGAGLVGVM
                     YILDEPSIGLHQRDNERLLGTLRHLRDIGNTVIVVEHDEDAIRMADYVVDIGPGAGVH
                     GGQIVAQGTAAEVMAHPDSLTGKYLSGRVKIKVPAKRTPRDKKLSLKIKGARGNNLRN
                     VDLDIPIGLLTCVTGVSGSGKSTLINNTLFPLSATALNGATTLEAAAHDSIDGLQHLD
                     KVVDIDQSPIGRTPRSNPATYTGLFTPIRELFAGVPESRSRGYGPGRFSFNVKGGRCE
                     ACQGDGLIKVEMHFLPDIYVPCDVCKSKRYNRETLEVKYKGKSITEVLDMTIEEARVF
                     FDAVPALARKLQTLMDVGLSYIKLGQSATTLSGGEAQRVKLSRELSKRDTGKTLYILD
                     EPTTGLHFADIQQLLDVLHRLRDHGNTVVVIEHNLDVIKTADWLVDLGPEGGSKGGQI
                     IAVGTPEEVAEMPQSHTGYFLKPLLERDRD"
     gene            complement(2993..3457)
                     /gene="bfr"
                     /locus_tag="VFF22_03540"
     CDS             complement(2993..3457)
                     /gene="bfr"
                     /locus_tag="VFF22_03540"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HZX15926.1"
                     /translation="MKGHIEVIDYLKILFKGELAARDQYFVHSRLYEDWGFSKLYERI
                     NHEMEEETQHADAILKRILFLEGTPDMVPDSFRFGQTVPEMLKLDLALEYHVRAALSK
                     GIALCEQHQDYQTRDILLVQLKDTEEDHAYWLEVQLGLIDRIGLQNYLQTQM"
     gene            complement(3617..>4001)
                     /gene="rplQ"
                     /locus_tag="VFF22_03545"
     CDS             complement(3617..>4001)
                     /gene="rplQ"
                     /locus_tag="VFF22_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017278734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HZX15927.1"
                     /translation="RHRKSGRHLSRTSAHRKAMFQNMAVSLFEHELIKTTLPKAKELR
                     RVAEPLITLAKEDSVANRRLAFDRTRSKAIVGKLFNDLGKRYATRQGGYLRILKCGFR
                     TGDNAPMAYVELVDRPVAGAAVDAE"
BASE COUNT          857 a         1267 c         1156 g          721 t
ORIGIN      
        1 agcagtaccc atgcttacgg cacagcagtg acgctttgcc gcgcccgcct cccgagccgt
       61 atactcggag gttttccgcc cgccatgcga ggccgttgtt tgtggacaag attctgatac
      121 ggggtgcgcg cacccataac ctgaaaaaca tcgacctaac cctgccacga gacaagctga
      181 tcgtgatcac cggcctgtcc ggttctggca agtcgtcact ggccttcgac accctctacg
      241 ccgagggcca gcgccgctat gtcgagtcgc tgtcggccta cgcccggcag tttctgtcga
      301 tgatggaaaa gcctgatgtc gacaccatcg aaggcctgtc gccggcgatc tccattgagc
      361 agaaatccac ctcgcacaac ccgcgctcca ccgtcggtac cattaccgag atctacgact
      421 acctacgcct gctctatgcc cgcgccggta ttccgcgctg cccggaccac gacctgccac
      481 tggaagcgca gaccgtcagc cagatggtcg accaggtgct ggccctgcct gaaggcagca
      541 agctgatgct gctggcgcca gtaattcgcg aacgcaaagg cgagcacctg gcgatcttcg
      601 acgaactgcg cgcccagggt tttgtccgcg tacgggtcaa cggcaaaatt tacgagctgg
      661 acgaactgcc caagctcgac aagcagaaga agcacagcat cgatgccgtg gttgaccgtt
      721 tcaaggcacg cggcgatctg cagcagcgcc tggccgaatc cttcgagacc gcactgaagc
      781 tggccgacgg cctggccctg atagcaccaa tggaaggcga ggaaggtgaa gaaatcatct
      841 tctccgcacg cttcgcctgc ccggtgtgtg gccactcgat cagcgagctg gaacccaagt
      901 tgttctcctt caacaacccg gccggcgcct gcccgacctg cgatggcctg ggggtaaaac
      961 agttcttcga caccaagcgc ctggtcaatg gcgaactgac cctggccgag ggtgcgattc
     1021 gcggctggga caggcgcaac gtctattact tccagatgct tggctccctc gccgcgcatt
     1081 acggcttcag cctggaagtg ccattcgacg agctcagcgc cgaacaccag aaatgcatcc
     1141 tgtttggcag cggcacgcag aacgtcgact tcaagtacct gaatgaccgt ggcgatatcg
     1201 tcaagcgctc gcaccccttc gaaggcattg tgccgaacct ggagcggcgc taccgcgaaa
     1261 ccgaatccac cagcgtacgc gaggagctgg ccaagttcct cagcacccag gcctgccagg
     1321 attgccgcgg tacccgtctg cgccgcgaag cccgccacgt atgggtcggc gagaagaccc
     1381 taccggcggt cagcggcatg ccaattggcg acgccaccga gtatttcggc agcctggcgc
     1441 tacccggccg ccgtggcgaa atcgccgaga agatcctcaa agaaatccgc gagcgcctgc
     1501 agtttctggt caacgtcggc ctcgactacc tgaccctcga ccgcagcgcc gatacattat
     1561 ccggcggcga ggcgcagcgt attcgcctgg ctagccagat cggtgccggc ctggtcgggg
     1621 tgatgtatat cctcgacgag ccgagcattg gcctgcatca gcgtgacaac gaacgcctgc
     1681 tcggcaccct gcgccacctg cgcgatatcg gcaacacggt gatcgtggtt gaacatgacg
     1741 aagacgccat tcgcatggcc gactacgtgg tggatatcgg cccgggtgcc ggcgtgcacg
     1801 gcgggcagat cgtcgcccag ggcacggctg ccgaagtcat ggctcaccca gactcgctga
     1861 ccggcaaata cctctccgga cgcgtgaaga tcaaagtgcc ggccaagcgc acgccacggg
     1921 ataagaagct ctcgctgaag atcaaaggcg cgcgcggcaa caacctgcgt aatgtcgacc
     1981 tggacattcc gatcggcctg ctcacctgcg tcaccggcgt gtccggctct ggcaagtcga
     2041 cgctgatcaa caacaccctg ttcccactca gcgccaccgc gctgaatggc gcgaccaccc
     2101 tggaagcggc ggcccacgac agcatcgacg gcctgcagca cctggacaag gtggtggaca
     2161 tcgaccagag tccgatcggc cgcacgccgc gctcgaatcc ggcgacctac accggtttgt
     2221 ttacgccgat ccgcgaactg ttcgccggag tgccggaatc gcgctcgcgc ggttacggcc
     2281 cggggcgctt cagcttcaac gtcaaaggcg gacgctgcga ggcctgccag ggcgacggcc
     2341 tgatcaaggt ggaaatgcac ttcctgccgg acatctacgt gccgtgcgac gtgtgcaaga
     2401 gcaagcgata caaccgcgaa accctggaag tgaaatacaa gggcaagagc atcaccgaag
     2461 tgctcgacat gaccatcgag gaagcacggg tgttcttcga tgccgtgccg gccctggcgc
     2521 gcaagctgca gacgctgatg gatgtcggcc tgtcctatat caagctgggc cagagcgcga
     2581 ccaccttgtc cggtggcgag gcgcagcggg tcaaactgag ccgcgagctg agcaagcgcg
     2641 acaccggcaa gaccctgtat atcctcgatg agccgaccac cggcctgcac ttcgccgata
     2701 tccagcaact gctcgacgtg ctgcatcgcc tgcgcgacca cggcaacaca gtggtggtga
     2761 tcgagcacaa cttggacgtg atcaagacgg ccgactggct ggtagatctc ggcccggaag
     2821 gtggctcaaa aggcggccag atcatcgccg tcggcacgcc cgaagaagtc gccgaaatgc
     2881 cgcaatcgca caccggctac ttcctcaaac cgctgctgga acgtgatcgc gactaagccc
     2941 gcgcccaaga aaaagccccg cacatgcggg gctttttctt ggctgcagaa tcttacatct
     3001 gcgtctgcag gtagttctgc aaaccaatcc ggtcgatcaa gcccagctga acttccagcc
     3061 aataggcgtg atcctcctca gtgtccttga gttgcaccag cagaatgtcg cgggtctggt
     3121 aatcctgatg ctgctcgcac agggcaatgc ctttgctcaa ggccgcgcgc acgtgatatt
     3181 ccagcgctag gtcgagcttg agcatctccg gcaccgtctg gccaaagcgg aagctgtcgg
     3241 gaaccatgtc cggcgtgcct tcgagaaaca gaatgcgctt gaggatggca tcggcgtgct
     3301 gagtttcctc ttccatttca tggttgatcc gctcgtacag cttgctgaaa ccccaatcct
     3361 catacagacg cgaatgcacg aagtactgat cacgggctgc caactcgccc ttgaacaaga
     3421 tcttcaggta atcgatgact tctatatggc ctttcatggg tatttcctta gcaagcgaca
     3481 aacaaggatc aatgctggcc gcacgcggcc tgcgggtcaa gcgcgatgca tacaccgata
     3541 gagtgagagc aaagcacgct agatcgtagc cataaaaaaa ccgggccaag gcccggtttc
     3601 ttatacaacc tacgacttac tcagcgtcta cagctgcacc agcgaccgga cggtcaacca
     3661 gctcaacata agccattgga gcattgtcgc cagtacggaa accgcacttg agaatacgca
     3721 ggtaaccgcc ctgacgggtg gcgtagcgct tgcccaggtc gttgaacagc ttgccgacga
     3781 tggctttcga acgagtacgg tcgaaggcca gacgacgatt ggcaacgctg tcttctttag
     3841 ccagagtaat cagcggctca gcaacgcgac gcagctcttt ggctttcggc agagtagttt
     3901 tgatcagctc atgctcgaac agcgataccg ccatgttctg gaacatggcc ttgcggtgtg
     3961 cgctggtgcg gctgaggtga cggccacttt tacgatgacg c
//
LOCUS       DASRUM010000049         8564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_528102, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000049 DASRUM010000000
VERSION     DASRUM010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8564
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528102"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1439
                     /locus_tag="VFF22_03550"
     CDS             51..1439
                     /locus_tag="VFF22_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX15928.1"
                     /translation="MHDPHNERMSGSETRAAGGLALVFAFRMLGMFMVLPVLATYGME
                     LQCSTPALIGLAIGAYGLTQAFLQIPFGMLSDRIGRRPVIYVGLLIFAAGSLLAANAD
                     SIYGVIAGRILQGAGAISAAVMALLSDLTREQHRTKAMAMIGMSIGLSFAVAMVVGPL
                     LTRAFGLSGLFLATAAMALLGIVIVAGLVPSSAGPLQHRESGVNKQALLPTLKHADLL
                     RLDFGILVLHAILMASFIALPLALVEQGGLAKEEHWWVYLTALLVGFFGMVPFIIYGE
                     KKRRMKHVLLGAVSVLLLCELFFWWFGTSLRALVLGTVVFFTAFNLLEASLPSLISKV
                     APAGGKGTAMGVYSTSQFLGAAIGGILGGWLYQHYSLSGVFAGCAVLALFWLAFAVTM
                     REPPYVTSLRLPLSAAALQEVGLAERLQAVPGVADAVVVRDEAAIYIKVDTQQLDRTS
                     LERLIEPAPVAC"
     gene            1457..1951
                     /locus_tag="VFF22_03555"
     CDS             1457..1951
                     /locus_tag="VFF22_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007966163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZX15929.1"
                     /translation="MARGVNKVILVGTCGQDPETRYLPSGNAVTNLSLATSEQWTDKQ
                     TGQKVEKTEWHRVSLFGKVAEIAGEYLRKGSQVYIEGKLQTREWEKDGVKRYTTEIIV
                     DMQGTMQLLGGRPDNAGGGDSAPRQQRPAPQREPQQSAPRPAPQQAPQPAADFDSFDD
                     DIPF"
     gene            1974..2858
                     /locus_tag="VFF22_03560"
     CDS             1974..2858
                     /locus_tag="VFF22_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HZX15930.1"
                     /translation="MRMRLMLLGGGNALGQALIRLGAEEDIGFLAPKPPESGWDPASL
                     TQLLDDTRPDALINLAYYFDWFQAEMVSEAHLAAQERSVERLAELCQHHQIRLLQPSS
                     YRVFDGSRATAYSEKEEPVPLGVRGQALWRFEQRVRAVCPRHVLLRFGWLLDDSANGL
                     LGRFLVRAAQDEPLLLADDRRGNPTPVDDAARVILAVLKQLDCQTPLWGTYHYGGHEA
                     TTSLALGQAVLSEARNFRSNLVDEVSPQAHSARPDAAEEPQHAVLSCKKILHTFGIKP
                     RAWRSGLPSLLDRYYRHV"
     gene            2851..3786
                     /locus_tag="VFF22_03565"
     CDS             2851..3786
                     /locus_tag="VFF22_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011336148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZX15931.1"
                     /translation="MSDSRPVLVTGGAGFIGSHLVDALLAQGYPVRVLDNLSMGKLAN
                     LPLDNPHLQFIQGDVADAAVVAQAVAGCAAVAHLAAVASVQASVDDPVATHQSNFVGT
                     LNVCEAMRLHGVKRVLFASSAAVYGNNGEGLAIDEATPKAPLTPYAADKLASEHYLDF
                     YRRQHGLEPAIFRFFNIFGPRQDPSSPYSGVISIFTQRVQQGLPISVFGDGEQTRDFF
                     YIEDLLALLTQALTAPQVVDGAVNVGWSQAVSLNQLLAQIGGLCGGLPAVNYQAARAG
                     DIRHSRADNQRLQAHYQLPTATPMREGLRRLLAQS"
     gene            complement(3847..4533)
                     /locus_tag="VFF22_03570"
     CDS             complement(3847..4533)
                     /locus_tag="VFF22_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HZX15932.1"
                     /translation="MRTSLFTASLLALAIAAPLAHAHQAGDIIVRAGAITVDPQESSS
                     DIWVGALGTDVAGTKATLDSDTQLGLNFAYMLTNNIGIELLAATPFSHNVGVKGMPGG
                     FAGLNGKLGELKHLPPTLSVVYYPLDASSAFQPYVGAGINYTWFFDTELSSEAEDKGF
                     SGLDMKDSWGLAAQVGMDYMLTDNIMLNAQVRYIDIETTGTTHILGDKVKVDVDVDPF
                     VYMVGLGYKF"
     gene            4774..5544
                     /locus_tag="VFF22_03575"
     CDS             4774..5544
                     /locus_tag="VFF22_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 domain-containing protein"
                     /protein_id="HZX15933.1"
                     /translation="MIPLPKRLLAVALVLASAPLAATPLDAALDESQQLAADAKASQA
                     RVEQLDDASREMLTEYRNALQQAEALQGYNAQLRELVAAQRKELTGYQEQLDGIERTQ
                     EAVTPQMRRMVEVLGEFIAADLPFLPDERSDRLAQLQDLLPRADVSLAEKYRRILEAY
                     QVESDYGRTLEAWRGELPSEGTSRSVEFLRLGRVMLYFQTLDGHESGWWNPQTRSWQI
                     LDGSARRPLRQAIAIARQEQAPAVLDLPVKTLALEAKP"
     gene            5541..6887
                     /locus_tag="VFF22_03580"
     CDS             5541..6887
                     /locus_tag="VFF22_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZX15934.1"
                     /translation="MNRRVLAFTLALLPALAGAAEPLSPDQLLQRIRSERAAEVSAMQ
                     SREQAFIAERGERAQLLATARSALATQKAEAEQLKAEFDRQETLLAEQEKLLSQRAGH
                     LGELFGVVRQSAGDVAGQWQDSLLNAQYPERLARLKALAESRTLPSAADLDGYWMLLL
                     EDLTASGRVEQLQLPVVAVDGTRNTQDVLRVGAFSAYGADAFLRYDADAGELLAPPRQ
                     PSGLGQVADYLDSSGAVASLPVDPSRGTLLAQLQREPGLWDRVQQGGLVGWVILVLGA
                     FGLLLAIWRMVYLTRVGRGVSAQMHDLSAPRTDNPLGRIIGVLGPKPQLADLETLELK
                     LDEAILQETPPLEKGQGLLKLLSAVAPLLGLLGTVTGMIVTFQAITQGGGGDSRLMAD
                     GISQALVTTVLGLVVAIPLLFLHTLLASRSKGLIQLLEQQSAGLIALHLSGAPRRD"
     gene            6880..7428
                     /locus_tag="VFF22_03585"
     CDS             6880..7428
                     /locus_tag="VFF22_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003304037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZX15935.1"
                     /translation="MTDWLALWLRLVDSGHALLDFMSAGGVVMWALAGLCVVFWTLVF
                     ERFWYMRRVFPEWVCERRQAWQQVLSDDTGNWQRAVRSAWLAQAQQHLLGPLRLSKTL
                     VAMYPLLGLLGTVSGMVAVFDVLAINGTGNPRGMAAGVWQATLPTMAGMVLAISGLFS
                     LARLERDARRALERLADQLRHD"
     gene            7439..7849
                     /locus_tag="VFF22_03590"
     CDS             7439..7849
                     /locus_tag="VFF22_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZX15936.1"
                     /translation="MRMRRHHQQDEDTGIDLTPMLDVVFIMLIFFIVTSSFIKEAGVE
                     VQRPQAETASPQDKGNILIAITADGQVWMDKKVVDVRSVRAHVERMRVDQPDGAVVVQ
                     ADQDARTGLVVQVMDQARLAGVHDVALAATAGAL"
     gene            7849..8532
                     /locus_tag="VFF22_03595"
     CDS             7849..8532
                     /locus_tag="VFF22_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZX15937.1"
                     /translation="MRLPLSFVAACVMALALFGLMLYMVAPPSSKPSEEPLTVANFVR
                     LDGDSSDTATRTRQQAPQPPQAQTPQTPTPPTPMTATPSANLPALDLPVPSLSTGIAV
                     NSAPTPSLTWLAAGASAPSAPAPSDAGGQPGGQESEVMPLNDVSPEYPRYALQRGIEG
                     HVKLAFTINRAGAVENVRVIEASPQNVFEREARRAAVRWRFAPRTEGGLAVAREAVKT
                     LYFRLEGAR"
BASE COUNT         1448 a         2563 c         2830 g         1723 t
ORIGIN      
        1 taagcatggg tactgctaga atcgccgcct attttcacag agcgttttcc atgcacgatc
       61 cgcacaacga gcgcatgagc ggcagtgaaa cccgcgcggc cggtggtctg gcgctggtgt
      121 tcgcgttccg tatgctcggc atgtttatgg tgctcccggt gctggcgacc tatggcatgg
      181 agttgcagtg cagcacaccg gcactgatcg gcctggcgat aggcgcctac ggtttgaccc
      241 aggcgtttct gcaaattcca tttggcatgc tcagtgaccg catcggccga cggccggtga
      301 tctatgtcgg cttgctgatt ttcgccgccg gcagcctgtt agcggccaat gccgactcga
      361 tttacggggt gattgccgga cgcattctgc agggcgccgg ggcgatttcc gcggcggtga
      421 tggcgctgct ctccgatctg acccgcgaac agcatcggac caaggccatg gcgatgatcg
      481 gcatgagcat cggtctgtcg tttgccgtgg cgatggtggt tggcccgttg ttgacccgtg
      541 cctttggcct atccggtctg tttctcgcca ccgctgccat ggcgctgctc ggcatcgtta
      601 tcgttgccgg gctggtgccg agttctgccg ggccgttgca gcatcgcgag tccggggtga
      661 acaagcaggc gttgctgccg acgctcaagc acgcggatct actgcgtctg gatttcggca
      721 ttctggtgct gcacgcgatt ctgatggcca gctttatcgc cttgccgttg gcgctggtgg
      781 agcagggcgg cctggccaag gaagagcact ggtgggtcta tctgaccgcg ctgctggtgg
      841 gcttcttcgg catggtgccg ttcattattt acggcgagaa aaaacgccgg atgaagcatg
      901 tgctgctcgg cgcggtcagc gtgctgctgc tgtgtgagct gttcttctgg tggttcggca
      961 ccagcctgcg cgcgctggtg ctcggcacgg tggtgttctt caccgcattc aacctgctgg
     1021 aagcctcgtt gccgtcgctg atcagcaagg tcgctccggc tggtggcaaa ggcacggcga
     1081 tgggcgtgta ttccaccagt cagttcctcg gcgcggccat cggcggcatc ctcggtggct
     1141 ggctctacca gcactacagc ctgtcgggag tgtttgccgg ctgcgcggtg ctggctcttt
     1201 tttggctggc ctttgctgtt actatgcgcg aaccgccgta tgtcaccagc ctgcgtctgc
     1261 cgctttcagc tgcggccttg caagaggtag ggctggccga gcgtttacag gcggtgcccg
     1321 gagtggcgga tgccgtagtg gtacgcgatg aagcggccat ctatatcaaa gtggataccc
     1381 aacaattgga ccgcacgtcc ctcgagcgcc tcatagagcc ggcgccggtg gcgtgctgaa
     1441 agcctaggag aacgttatgg cccgtggggt taacaaagtc attctggtcg gtacctgcgg
     1501 acaggacccg gaaacccgct acctgccgag cggtaacgcg gtcaccaacc tgagcctggc
     1561 gaccagcgag cagtggactg acaagcagac cggtcagaag gtcgagaaaa ccgagtggca
     1621 ccgtgtgtcg ctgttcggca aggttgccga gatcgccggc gaatacctgc gcaaaggttc
     1681 gcaagtgtat atcgaaggca agctgcagac ccgcgagtgg gaaaaagacg gcgtcaagcg
     1741 ctacaccacc gaaatcatcg tcgacatgca gggcaccatg cagctgcttg gcggccgtcc
     1801 ggacaacgcc ggtggcggcg actccgctcc acgtcagcag cgccctgcgc cgcagcgcga
     1861 gccacagcag tcggcaccac gcccggctcc gcaacaagcg cctcagccag cggcggattt
     1921 cgacagcttc gatgacgata tcccgttctg atcagctgag ttagcgctcg atcatgcgta
     1981 tgcggctgat gctgctgggt ggcggtaatg cgctggggca ggccctgatc cgcctcggcg
     2041 ccgaggaaga cattggcttt ctcgcgccga aaccgccgga gtcgggctgg gatcctgcca
     2101 gcctgaccca gttgctcgat gacacccgtc ccgatgccct gatcaacctt gcctactatt
     2161 tcgactggtt tcaggccgaa atggtgagtg aggcgcatct tgctgcccag gaacgttccg
     2221 tcgagcgtct ggccgaactc tgccagcacc accagatccg tctgctacaa ccctccagct
     2281 atcgtgtgtt cgatggttcg cgggcgaccg cctacagcga gaaggaagag ccggtaccgc
     2341 tgggcgtgcg tggtcaggca ctgtggcgct tcgagcagcg cgtgcgggca gtctgtccac
     2401 ggcatgtgct gctgcgtttt ggctggttgc tggatgacag cgccaacggc ctgctcgggc
     2461 gctttcttgt ccgtgccgcg caggatgagc cgctgctgct ggccgatgat cggcgcggta
     2521 atccgacccc ggtcgacgat gccgcgcggg tgattctcgc tgtgcttaag cagctggatt
     2581 gccagacgcc gttgtggggc acctaccact acggcgggca tgaggcgacc acctcgctgg
     2641 cgttgggcca ggcggtgttg agcgaagcgc gcaacttccg cagtaatctg gtcgatgagg
     2701 tcagtccgca ggcgcacagc gcgcgtccgg atgctgccga agagccgcag catgcggtgc
     2761 tgtcctgcaa gaaaattctg cacaccttcg gcatcaagcc gcgtgcctgg cgctcgggtt
     2821 taccgagcct actggatcgt tattaccgcc atgtctgatt cgcgtcctgt actggttacc
     2881 ggcggtgccg gctttatcgg ctcgcacttg gttgatgcgc tgctggcgca gggttacccc
     2941 gtgcgggtgt tggacaacct gtcgatgggc aaactggcca atctgccgct ggataacccg
     3001 catttgcagt ttattcaggg cgatgtcgcc gatgcggcgg tggtggcgca ggcggttgcc
     3061 ggttgcgcgg ctgtggcgca cctggcggcg gtggcctcgg tgcaggcctc ggtggatgat
     3121 ccggtggcga cccatcagag caacttcgtt ggcacattga atgtctgtga ggcgatgcgt
     3181 ctgcatggcg ttaaacgcgt gctgtttgct tccagtgcgg cggtgtatgg caacaatggc
     3241 gaaggcctgg cgattgatga agccacgccc aaggcgccgt tgacacctta tgcggcggac
     3301 aagctggcca gtgagcacta cctggatttc tatcgccgcc agcatggtct ggagccggcg
     3361 atctttcgtt tcttcaatat ctttggcccg cggcaggatc catcctcgcc gtactccggg
     3421 gtgatcagca tctttaccca gcgcgtccag caggggctgc caatcagtgt gttcggtgat
     3481 ggcgagcaga cccgcgactt cttctatatc gaggacttgc tggcgctgct gacccaggcg
     3541 ctgactgcgc cgcaggtggt ggatggtgcg gtgaatgtgg gctggagcca ggcggtcagc
     3601 ctgaatcagc tgctggcgca gatcggtggg ctgtgcggcg gcttgcctgc ggtcaattac
     3661 caggctgcgc gtgccggtga tattcgccat tcgcgggctg ataaccagcg cttgcaggcg
     3721 cattaccagc tgcccacggc aacgccaatg cgcgaaggcc tgcggcgcct gctggcgcag
     3781 tcttgagctt tctctaagcg acaaaaaacc ggccatttag gccggttttt gtttggctgc
     3841 tgattcttag aatttgtaac ccaggccgac catataaacg aacggatcta cgtccacatc
     3901 gactttgacc ttgtcaccca ggatgtgggt agtgccggtg gtttcaatgt cgatatagcg
     3961 cacctgggcg ttgagcatga tgttgtcagt cagcatgtag tccatgccga cctgcgccgc
     4021 caggccccag gaatccttca tgtccaggcc actaaagcct ttatcttcgg cctcgctgct
     4081 cagctcggtg tcgaagaacc aggtgtagtt aatgccggcg ccgacatagg gctggaaggc
     4141 cgagctggca tccagcgggt agtacaccac gctcagggtt ggtggcaggt gcttgagttc
     4201 gccgagtttg ccgttcaggc cggcaaaacc gccaggcatg ccttttacgc cgacgttatg
     4261 gctgaacggg gtggccgcta gcagctcgat gccgatgttg ttggtcagca tataagcgaa
     4321 gttcaggccc agctgagtat cgctgtccag ggtcgctttg gtgccggcca cgtcggtgcc
     4381 gagcgcgcca acccagatgt cgctgctgct ttcctgtggg tcaacggtga ttgcaccggc
     4441 ccgcacgatg atgtcgccag cttggtgcgc gtgtgccagg ggggcggcaa tggccagggc
     4501 cagcagtgag gcggtaaaca gtgatgtacg catggtgtgc tcccataaag catcggttcg
     4561 tattggctga gtgcgatgtt aggggcgcgg aaaaagcgac tgttgatcca gctcaataaa
     4621 gtagctagct atctaaattt tcttttgcgg ggcatgaggg acgacgcttg tggcgttttg
     4681 ggtgcgcggc aggcgtaacg tgggttgctt gagaggcgta aggcagttga taatagttct
     4741 ctttatgcac gttctcttaa caaggaatgc cccatgatcc ctctgccgaa gcgcctgctg
     4801 gcagtcgctc tggttctggc cagtgcgccg ctcgcggcga cccctctgga tgccgcgctg
     4861 gatgaaagtc agcagctggc tgccgatgcc aaggcctcgc aggcgcgcgt cgagcagctc
     4921 gatgacgcca gtcgggaaat gctcaccgag taccgtaacg ccttgcagca ggccgaagcg
     4981 ttgcaaggtt ataacgctca gttacgcgaa ctggtggcgg cgcagcgcaa agagctgacc
     5041 ggctatcagg agcagctgga tggcatcgag cgcactcagg aggcggtaac gccacagatg
     5101 cgccgcatgg tcgaggtgct gggcgagttt atcgctgccg atctgccgtt cctgccggat
     5161 gagcgcagtg atcgcctggc ccagctgcag gacttgctgc cgcgtgcaga tgtcagcctg
     5221 gccgagaaat accgacgcat ccttgaggct tatcaggtgg agagcgacta cggccgcacc
     5281 ctggaagcct ggcgcggtga gctgccgagt gaaggcacgt cgcgcagcgt cgaattcctc
     5341 cggcttggcc gggtgatgct gtatttccag accctcgatg gccatgaaag tggctggtgg
     5401 aacccgcaaa cccgcagttg gcagatcctc gacggcagcg cccgtcgtcc gttgcgccag
     5461 gcgattgcga ttgcccgcca ggagcaggcc ccggctgtgc ttgacctgcc cgtgaagacc
     5521 ctggcgctgg aggccaagcc atgaatcgtc gcgttctcgc ttttaccctg gccctgctgc
     5581 ccgccctggc tggcgctgcc gaaccgctga gcccggatca actgctgcaa cgtattcgca
     5641 gcgaacgcgc ggctgaagtc agcgccatgc aaagccgcga gcaggccttt attgccgaac
     5701 gtggcgagcg tgcccagctg ctggccactg cgcgcagcgc cctggccaca caaaaggccg
     5761 aggccgaaca gctgaaggcc gaattcgacc gtcaggaaac tctgctcgcc gaacaggaaa
     5821 aactcctcag ccagcgcgcc ggtcacctcg gcgagttgtt tggtgtggtc cgtcagagcg
     5881 ccggtgatgt cgccggccaa tggcaggaca gcctgctcaa tgcccaatac cccgagcgcc
     5941 tggcgcgtct caaagcgttg gccgaaagcc gtacgttgcc ctcggccgcc gacctcgatg
     6001 gctactggat gctgctgctc gaagacctca ccgccagtgg ccgggttgag caactgcaat
     6061 tgccggtggt tgccgtcgac ggcacacgta atacgcagga tgtgctgcgt gtcggcgcgt
     6121 tctccgccta cggcgccgat gccttcctgc gttatgacgc cgatgccggc gagttgcttg
     6181 cgccgccgcg tcagccgtcc ggcctgggtc aggtcgcgga ttatctggac agcagcggcg
     6241 cagttgccag cctgccggtt gacccaagcc gcggcacctt gctggcgcaa ttgcagcgcg
     6301 agcctggctt gtgggatcgc gtacagcagg gtggtctggt cggctgggtg attctggtgc
     6361 tcggcgcatt cggtctgctg ctggcgatct ggcgcatggt ctacctgacc cgtgtgggtc
     6421 gtggtgtcag cgcgcagatg catgacctga gtgcgccgcg caccgataac ccgctggggc
     6481 ggatcatcgg cgtgctgggt cctaaaccgc agctggcgga tctggaaacc ctggagctga
     6541 agctcgacga agcgatcctc caggaaaccc cgccgctgga aaaaggccag ggcctgctca
     6601 agctgctcag cgccgtggcg ccactgcttg gtctgctcgg caccgtgact gggatgatcg
     6661 tcaccttcca ggcgattacc cagggcggtg gcggcgattc gcgactgatg gccgacggta
     6721 tctcgcaagc gctggtgacc acggtgttgg gtctggtggt ggcgattccg ctgctgttcc
     6781 tgcacaccct gttggcgagc cgcagcaagg gcctgattca gctgctggag cagcaaagcg
     6841 ccgggctgat tgccctgcac ctgtccgggg cgccgcgccg tgactgattg gctggcgcta
     6901 tggttgcgcc tggtggacag cggccatgcc ttgctcgatt tcatgagcgc cggtggcgtg
     6961 gtgatgtggg cgttggctgg cctgtgcgtg gtgttctgga ccctggtatt cgagcgcttc
     7021 tggtacatgc gccgggtgtt cccggagtgg gtctgcgagc gccgccaggc ctggcagcag
     7081 gtgctgagcg acgacactgg caactggcag cgcgcggtgc gcagtgcctg gctggcgcaa
     7141 gcgcagcagc acctgctcgg gccattgcgt ctgagcaaga ccctggtggc gatgtacccg
     7201 ctgctgggct tgcttggcac ggtcagcggc atggtcgcgg tgttcgacgt gctggccatc
     7261 aacggcaccg gtaacccgcg cggcatggcg gccggggtct ggcaggcgac cctgccgacc
     7321 atggccggca tggtattggc gataagtgga ttgttcagcc tggcgcgcct cgaacgcgac
     7381 gcccgccggg ccctagagcg gctggctgac cagctgcgtc acgactgaga ctcaacacat
     7441 gagaatgcgc cgtcaccacc agcaagacga agacaccggc atcgacctga cgccgatgct
     7501 cgacgtggtc tttatcatgc tgatcttctt tatcgtcacc agctccttta tcaaggaagc
     7561 cggcgtcgaa gtgcagcggc ctcaggcgga aaccgccagc ccgcaggaca aaggcaatat
     7621 ccttatcgcc atcaccgccg atgggcaggt gtggatggac aagaaagttg ttgatgtgcg
     7681 cagcgtgcgc gcccatgtcg agcgcatgcg cgtcgaccag ccggacggtg ccgtggtggt
     7741 tcaggccgat caggacgcgc gtaccggcct ggtggtgcag gtgatggatc aggcacgcct
     7801 ggccggagtg catgacgtgg ctctggccgc tacggcggga gcgctttgat gcgtttgcca
     7861 ctgtcctttg tcgcggcctg tgtgatggcg ctggcgctgt tcggtctgat gctttacatg
     7921 gtcgcgccgc ccagcagcaa gccgagtgag gaaccgctga cggtggccaa tttcgtccgt
     7981 ctcgacggcg actccagcga caccgcgacg cgcacccgcc agcaggcacc gcaaccgccg
     8041 caagcacaaa caccgcaaac cccgacaccg ccgacgccga tgacggccac cccgagcgcc
     8101 aacctgccgg ccctggatct gcctgtgccg agcctgagca ccgggattgc cgtgaacagc
     8161 gcgccgacac caagccttac gtggctggca gcgggtgcct cagcgcccag tgcacccgca
     8221 ccgagcgatg ccggtggtca accgggcggt caggaaagtg aagtgatgcc gctcaacgat
     8281 gtcagcccgg aatacccacg ctacgcctta cagcgcggca ttgaagggca tgtgaagctg
     8341 gcgttcacca tcaatcgcgc cggtgctgtc gaaaacgtgc gcgtcatcga agccagtccg
     8401 cagaacgtgt tcgagcgcga agcccgccgt gcagccgtac gctggcgctt tgcgccgcgt
     8461 accgagggtg gtctggcggt ggcccgcgaa gcggtgaaaa ccctgtactt ccgcctggaa
     8521 ggagctcgct gatatgtatc gtctactgct gctgatcgcg ctgt
//
LOCUS       DASRUM010000050        21589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_363772, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000050 DASRUM010000000
VERSION     DASRUM010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 21589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21589
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363772"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VFF22_03600"
     CDS             complement(<1..595)
                     /locus_tag="VFF22_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZX15938.1"
                     /translation="MKLETLAIHAGYSPDPTTKAVAVPIYQTSSFAFDDTQHGADLFD
                     LKVAGNIYSRIMNPTNAVLEERVAALEGGVGALAVASGMAAITYAIQTVAEVGDNIVS
                     VAKLYGGTYNLLAHTLPRFGIQTRFAAHDDIAALEALIDDKTKAVFCESIGNPAGNIV
                     DLAALAAAAHRHGVPLIVDNTVATPILCRPFEHGADIV"
     gene            complement(718..2514)
                     /locus_tag="VFF22_03605"
     CDS             complement(718..2514)
                     /locus_tag="VFF22_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZX15939.1"
                     /translation="MFWDSLPLPLLFVGALLVWVLYAFVREKWSPDIVAAIAVAALLI
                     TQLLTPGEVLSVLSNSAPVTIACMFVLSAALERTGCIDALGNWLGDLVGTSPIRVLTG
                     LTITALVISAFLNNTPVVAILTPVAISLARRAGTLPSKLLIPLSYATILGGTLTMIGT
                     STNILVDGVARKAGLEPFGMFEITGAGLILATVGMLYLLTIGRHLLPERETLSKQLRP
                     DLDRTFMTELLVPHDSPVIGKSLHEANLNGGSGLQVLKIFRDEQELTEPAHDTLLLAG
                     DQLVLHGQVKDVVELRESGHLIFNRSDAFETISSHDVILAEAIVGRNSRYSHRPMRDL
                     DLTARYGIAVLAVHRQDENIQGNLDDFELQFGDVMLVEGTPTQIKRFADNGELISLNA
                     VQERAFRRDKAPIAIIATLAVMILAAFGVMPIEGLAIIGAVTVLATRCLDVEDAYKAV
                     DWKILSLIFGMLAISIAMDKVGLVSLMVSNVMGLMPWAGPLLMLSFIYLFTSILTEVL
                     SNNAVAVLVTPIAIGMAQHLGVDPRAFVVAVMFAASASFATPIGYQTNTFVYNAGGYR
                     FTDFMKVGIPLNLLLWVVASFVIPWFFPLTPI"
     gene            2753..3448
                     /locus_tag="VFF22_03610"
     CDS             2753..3448
                     /locus_tag="VFF22_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HZX15940.1"
                     /translation="MRQLGVLGGMSWESTASYYRLLNQGVRERLGGLHSAPMLLHSVD
                     FAEIAELQRRGDWQAAGELLAKAAQGLQQAGATALLLATNTMHKVAPAIEAAVDIPLL
                     HIGDAVGQALQRQGVRRAALLGTRFTMQEDFYRQRLAERFAIEVITPDAAQMAEVDRV
                     IFAELCQGQFLPQAQAFYLDCLRTLQEQGAEVAILGCTEIGLLLDGLDAPLPLLDSTE
                     LHVAMGLEWLLGS"
     gene            complement(3514..4974)
                     /locus_tag="VFF22_03615"
     CDS             complement(3514..4974)
                     /locus_tag="VFF22_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HZX15941.1"
                     /translation="MKSLIELQFDNRFARLGDAFSTHVLPEPIAEPRLVVASPAAMAL
                     LDLQPSEADSEVFAQIFAGHKLWSTAEPRAMVYSGHQFGGYNPRLGDGRGLLLGEVIN
                     DASEYWDLHLKGAGQTPYSRMGDGRAVLRSSIREFLASEHLHALGIPSSRALCVTGSS
                     TPVWREKQESAAMVLRLAPSHVRFGHFEYFYYTQQHEQLKQLAEHVLHCHYADCLRQE
                     QPYLAMFREIIERNAAMIAYWQAYGFCHGVMNTDNMSILGITFDYGPYAFLDDFDAKH
                     ICNHSDDTGRYSFSNQVPIAQWNLAALGQALVPLVDVEALRETLGLFLPLYQAHYLDL
                     MRKRLGLLSADDGDEALVQRLLQLMQSSAVDYTLFFRELGDSPAAQAVARLRDDFTDL
                     AGFDTWAADYLARSAQDGDDQAKRRTRMHAANPKYILRNYLAQQVIEAAEQGDYAPVR
                     ELHQVLSQPFDEQPGFERYAQRPPEWGKHLEISCSS"
     gene            complement(5050..8415)
                     /gene="mscK"
                     /locus_tag="VFF22_03620"
     CDS             complement(5050..8415)
                     /gene="mscK"
                     /locus_tag="VFF22_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HZX15942.1"
                     /translation="MPSLRTYLAVALLGLCLSAPLLHAAEPPSRDEVQSSLDNLADRK
                     LPEAEQLVVKQTLEKTLALLDEKADSEQRRADLEKQLQQAPRLIGEAQRDYERLKNST
                     PTQVNLRYGKSALPQLEQLLADRNAQLNEWQKQIIDANSLVITAQTRPERAQAEISGN
                     QTRSQEINNILKSGRDASKPLSNERRNQLNAELAKLDAQTQLRRQELAGNSLLQDLGN
                     SRRSLLEERIKRLEQELLDLQNLINEKRRDLSEQTLAEQSREVEKAGTDSLLAQESAL
                     NLKLSDYLLRATDRLNELTRLNLQTKQQLDSLSQSDQALEEQISVLQGSLLLSRILYK
                     QQQALPTLKLDSSLPDQIADLRLYQFELNQQREAINNPSVYVDTLLSEQANEPNNQAL
                     RTDLLEMINTRRELIDRLNRELNALLNESITLQLNQKQLKTTADTLRATLDEQMFWIP
                     SNKPLDLEWFKSVPRLLERQIAELPWGANVRQLGAGLTERPLLFLPLLLLIGVLLWRR
                     GYLYRKLAELHQDIGHFKRDSQLHTPMAIMLNVLLALPGTLFLALCGFALQMDARGQN
                     ANLGEALFQMAQAWLVFYTLYRILADGGVAELHFRWGRSQVAFLHRQVRRLGLVVMAM
                     VAVVTVAKHQPAGLGDDVIGLAVVLTCYALMVWLLHKLLLTGPAREHASAFRMLIGMA
                     LSLLPLALIVAVGLGYYYTALKLSDRLINTLYLLAFWLVIEATFIRGLGVAARRLAYQ
                     RATTKRQAQSKDSGEGTETVVEEPTLDIEQVNQQSLRLIRLALLGTFIAGLYWVWADL
                     ISVFAYLDNIALYEYSSGTGDAATLVPISLSDVLGALVIIGITIALGRNLPGLLEVLV
                     LSRLKLAQGSAYATTTLLSYLIVAIGFVTTLSTLGVSWDKLQWLVAALSVGLGFGLQE
                     IFANFISGLIILFERPVRIGDVVTIGNLSGTVSKIRIRATTITDFDRKEIIVPNKTFV
                     TDQLINWSLNDTVTRVIVKIGVAYETDLPLARKLMMQAAIENPRVLRDPEPLLFFLTI
                     SASTFDYELRFHVRELGDRNAATDEILTRIALTFRENNVEMAFNQVEVMIKNLHGQEL
                     NLTSGQMVAATAAADAQQGQLPPTAPAPAIDPQ"
     gene            complement(8449..10194)
                     /locus_tag="VFF22_03625"
     CDS             complement(8449..10194)
                     /locus_tag="VFF22_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012721847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HZX15943.1"
                     /translation="MDASTINNLFLIGGVLVAMSILVSAFGSRFGIPILVIFLAVGML
                     AGTDGPGGIIFNNYPLAYLVGNLALAIILLDGGMRTRVSSFRVALWPSLSLATVGVLI
                     TAGLTGVAAAWLFDLTWMEGLLIGAIVGSTDAAAVFSLLGGRGLNERVSATLEIESGS
                     NDPMAVFLTVTLIAMLASGQDGMSWNLLVQLLQQFGIGALFGLGGGWLLLNLINRMEL
                     ANGLYPLLVVSGGLIIFAITTAVGGSGILSIYLCGLLLGNRPIRSRHGILHMLDGLTW
                     LAQIGMFLVLGLLVTPHELLPIALPALGLALWMILFARPLSIFIGLLPFKGFHDRERA
                     FIAWVGLRGAVPIILAVFPLMAGLPNAQLFFNVAFFIVLVSLLLQGTSLPFAAKLLRV
                     TVPPEPAPVSRAGLDVHPTSQWELFIYRLGAEKWCIGAPLRELKMPDGTRIAALFRGK
                     ELLHPSGSTTLDAGDLLCVVGHEHDLPALGKLFSQAPTRGQDLRFFGDFVLEGDAELA
                     AIAALYGLKLEGAEGQLHLGRFIAHKIGGSPVIGDQVEWQGLTWTVAAMEGNKIRKVG
                     VRFPEGSRPGPGLFL"
     gene            complement(10335..12137)
                     /locus_tag="VFF22_03630"
     CDS             complement(10335..12137)
                     /locus_tag="VFF22_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZX15944.1"
                     /translation="MSESLLSSRNLAFELYEVLDAEGLTQRERFAEHSRETFDAAIDT
                     ARSIAENLFAPHNRKNDEHEPEYVDGAAVLIPEVKPAVDAFLEAGFLNATREFEVGGM
                     QLPNLLSQACFAHFQSANAATTSYPFLTMGAANLIETFGSDEQKQRFLQPMIDGRFFG
                     TMALTEPHAGSSLSDIRTRAEPAGDGTYRLKGNKIFISGGDHPLSENIVHMVLAKLPD
                     APAGVKGISLFIVPKFLVNDDGSLGKRNDVVLAGLFHKMGWRGTTSTALNFGDNGDCA
                     GYLVGKPHHGLAYMFQMMNEARIGVGMGAVMLGYAGYLYSLDYARQRPQGRLPDGKDP
                     TSGQVAIIEHADVRRMLLTQKAYVEGAFDLGLYAARLFDDTQTLATEDERRIALELLD
                     LLTPIVKSWPSEFCLKANELAIQILGGHGYTRDYPVEQYYRDNRLNPIHEGTHGIQSL
                     DLLGRKVSMNGGAALKQLLKLIHDTCQRAEAHASLTALRQPLEQLLARLQTVTLALLG
                     DLMSGKVNEGLANSALYLKVFGHTVIGWRWLEQAIRAEEGLARGNPADADFYQGKLQA
                     ARYFLTWEVPSCHHDLAILEARDDTCLGMQDAWF"
     gene            complement(12220..13263)
                     /locus_tag="VFF22_03635"
     CDS             complement(12220..13263)
                     /locus_tag="VFF22_03635"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HZX15945.1"
                     /translation="MSLQALLGTQLPLIQAPMAGVQLHGLAAAVSNAGALGSLPCAML
                     DAAGLRKELQALGEKTSKPYNLNFFCHTPPAIDSAREATWREALSPFYTELGINPATI
                     ASGPGRLPFSAEMADLLDEFRPAVVSFHFGLPSAELLVRVKACGAKVLSSATTVEEAL
                     WLQANGADAIIAQGLEAGGHRGMFLTDELNSQLGTFALLPQIVQAVQLPVIAAGGIAD
                     AAGVRAAMALGAAGVQIGTAYLCCDEASTSAIHRAALHSQAAQHTALTNLFSGRPARG
                     IVNRLMRELGPISPLAPAFPLATAAIAPLRSQAESQGSGDFSPLWAGQNVSGCKSIGA
                     AELTAELARGFAS"
     gene            13370..14218
                     /locus_tag="VFF22_03640"
     CDS             13370..14218
                     /locus_tag="VFF22_03640"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HZX15946.1"
                     /translation="MNYRHAFHAGNHADVLKHYVLSRLIALLSRKEAPFAYLDSHAGI
                     GLYDLRGDQASRTGEWLEGIARLWQATDVPDPLLDYLEVIRAMNPDGELRHYPGSPEL
                     ARLLTREQDRLHLNEKHPEDGRLLKENMHGDRRVAVHLGEGWYVPRALLPTREKRALL
                     LIDPPFEQVDEMERCAQSLSEAIGRMRQAVVAIWYPIKDLRQLRRFYSDLQKSKAPKL
                     LRIELYVHSTDEAARLNGSGLVISNPPWGLEDELKQILPWLAELLGQSQGGWRMDWLI
                     EESSAS"
     gene            complement(14215..15468)
                     /locus_tag="VFF22_03645"
     CDS             complement(14215..15468)
                     /locus_tag="VFF22_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZX15947.1"
                     /translation="MPAASVYISPDQLCVGLYIQLELAWWEHNFAFSNFKIKDEAQIS
                     ALRALGLERLRYDPARSTCPPLSASPAPVAADSVVAPTLSSQQQASLARAEKLKALRK
                     RLAEVDRKFVQASQQVKTLNQTLRSRPEDAIKQAGEIVGAMVETLLGGSGVVLHSINS
                     KTAEDSYLHSLNVTVLSLMLGRQLGLDSEDCHILGLGALLHDVGKLEVPSKVLLKSEP
                     LTRPEQQLLQLHCEFGLRMGHKLMLDDEVLRIINEHHEYCDGSGYPRQLREAAIGRLS
                     RLVCITNHFDNLCNPLNPRDGLSPHEALALMFKQYNARFDAVALKAFIRCMGIYPPGC
                     LVQLDDQRYGLVLGMNPSQPLKPTLIVYDAAVPKAEALIIDLEQEPRLSIASSLRPSQ
                     LPREALEYLNPRQQLSYFVEPSKGR"
     gene            complement(15573..15770)
                     /locus_tag="VFF22_03650"
     CDS             complement(15573..15770)
                     /locus_tag="VFF22_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative motility protein"
                     /protein_id="HZX15948.1"
                     /translation="MDLSSLTNQVSTQAAAQASALASLLLLRKTLELQAASIGGLVQA
                     ATPASAPSNPSNLGNSVDVMV"
     gene            complement(15800..16447)
                     /gene="msrA"
                     /locus_tag="VFF22_03655"
     CDS             complement(15800..16447)
                     /gene="msrA"
                     /locus_tag="VFF22_03655"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020305773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HZX15949.1"
                     /translation="MVLRSQILAHKLELPTAEQALPGRADVMTVPAAHFVNGNALQGP
                     FPAGLEQAVFAMGCFWGAERRFWQQPGVWSTAVGYAGGHTPNPTYDETCSGLTGHTEV
                     VLVVFDPKVTSYSALLKVFWEAHNPTQGMRQGNDIGSQYRSAIYCYGPEQLTAAQSSQ
                     AQFQAELSKAGYGAITTEVCEAPPFYYAEAYHQQYLAKNPGGYCGLGGTGACLPG"
     gene            complement(16513..19155)
                     /locus_tag="VFF22_03660"
     CDS             complement(16513..19155)
                     /locus_tag="VFF22_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016704477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZX15950.1"
                     /translation="MKSHPDAASRIAAEVVTQLPVPSRLGLLRFERLNEASWTLLYLD
                     SGCEQQFGLPAQELCSLLDAPYASLMEPTARYQLHDSIAQQLAERRHYSVQYRLHTPL
                     GSLTLMEVGEAFKQHNRHLLRGYLLVITEQSAPSPQPLELYEHSQAEPLAQQQRRQAQ
                     RQLIVTLARHHYGDNDPLREAAELISKSACELYGVSRVGIWNLNQHVLQSVALYQQDQ
                     GRHIDQADIDASPYPAYLQALHSGRALDAHDAHQDPRTCELSSSYLEPMGITAMLDAS
                     IRIAGEVVGVLCLEHIGAPRTWQADEIAFAGELADQYAQVLANQQRRSATNALYLFQR
                     AVEQSSSAFILLDRDGLVEYVNPSFTAITLYSAGEVQGRRLSEIPALENLSDLLFDTH
                     SGLVEHNSWQGEFKSRRKNLDPYWGQLSLSKVHADDGCLTHYIGIYEDVTASKQAQQH
                     IERLAFRDNLTGLGNRYAFIRSLERHFSEHPGKPISLLLVDIDNFKRINDSLGHQTGD
                     KLLVSLARRLRNTLHPNSVLARFASNEFAVLLDANQADSQLVAQKLLHTLDKPLFVDN
                     QLISVTGSIGLGCAPEHGDDPQTLMKHTGLALHKAKANGKNQLQVFTDVLNAEAHYKL
                     FVESNLRRALMQNELEVFYQPKLCLKSGRLLGLEALLRWHHPEKGMISPDKFISVAEE
                     TGLIIPIGKWVTRQACRMSKQLIAVGYTPLQMAINLSPKQFSDPDLAGEVAAILHEEQ
                     LDPALLELELTESLLLEATDDTRHQLNHLKSLGLTLAMDDFGTGYSSLSYLKKFPLDV
                     IKIDRSFIKDIPENQDDMEITSAVIAMAHNLKLKVVAEGIETVAQLNFLRRQQCDIGQ
                     GYLFDKPIASRDLLKSLKRYPNRG"
     gene            19287..19763
                     /locus_tag="VFF22_03665"
     CDS             19287..19763
                     /locus_tag="VFF22_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15951.1"
                     /translation="MATPTVFAAEPDTGLSSAEQGNYLLELKRLYLSPNDRQALLAHC
                     NDLLKTYALRAAYQVGQAQRQDLIYQLRQGESGELLLREETRGQQGTDIAVRNQRVPL
                     FGVDPFVRYECPANGISCVLHNPNDGSPMLTIVRDHKGAAELAKALSFLIRNLQKG"
     gene            complement(19864..21534)
                     /gene="aceF"
                     /locus_tag="VFF22_03670"
     CDS             complement(19864..21534)
                     /gene="aceF"
                     /locus_tag="VFF22_03670"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HZX15952.1"
                     /translation="MSELIRVPDIGGGEGEVIELMVKVGDRIEADQSVLTLESDKASM
                     EIPAPKSGVVKSVKVKLGDRLKEGDELFELEAEGAAQAPAAPVSAPAEAPKAAAAEAA
                     AAPAPAPAPAPAAQTSSVQDVHVPDIGSDGKAKVIEILIKVGDTVEADQSLITLESDK
                     ASMEIPSPAAGVVESISVKLEDEVGTGDFILKLKVAGAAAVPAAAPAPAAAPASDVHK
                     VPAGAHPAVAAEVGAIASLAAAAANVAAAPKRDGAKVHAGPAVRLLAREFGVELSAVP
                     GTGPKGRVLKEDVQAYVKAMMQKAKEAPAAGATGGAGIPPIPVVDFSKFGEIEEVAMT
                     RLMQVGANNLHRSWLNVPHVTQFDSADITELEAFRVAQKAVAEKAGVKLTVLPLLLKA
                     CAFLLKELPDFNSSLAPSGKAIIRKKYVHIGFAVDTPDGLLVPVIKNVDQKSLLQLAA
                     EAAVLAEKARSKKLSADDMQGACFTISSLGHIGGTGFTPIVNAPEVAILGVSKATIQP
                     VWDGKAFQPKLMLPLSLSYDHRVINGAAAARFTKRLGDVLADIRTMLL"
BASE COUNT         3893 a         6318 c         7160 g         4218 t
ORIGIN      
        1 cgacgatgtc ggcgccatgc tcgaacgggc ggcagaggat cggcgtggcc acggtgttgt
       61 cgacgatcag cggcacgcca tggcggtgcg cggcagcggc cagggcggcg aggtcgacga
      121 tattgccggc cgggttgccg atggattcgc agaacacggc tttggttttg tcgtcgatca
      181 gcgcttcgag ggcggcgatg tcgtcatggg cggcgaagcg ggtctggatg ccgaagcgcg
      241 gcagtgtgtg ggccagcagg ttgtaggtgc cgccgtagag cttggccacc gagacaatat
      301 tgtcaccaac ctcggccacg gtctggatcg cgtaggtgat ggcggccatg cccgaagcca
      361 cggccaacgc gccgacgcca ccttccagtg ctgccacgcg ctcttcgagc acggcgttgg
      421 tcgggttcat gatccgcgaa tagatgttgc cggcgacctt caggtcgaac aggtcggcgc
      481 cgtgctgggt gtcgtcgaag gcaaaggagc tggtctggta gatcggcacc gccaccgcct
      541 tggtggttgg gtcggggctg tagccggcgt ggatggccaa ggtttccaat ttcatgcgcg
      601 cgttccttca gggcagattg aaggttggca gcatgggcaa agcagcaagg cccggtcaat
      661 gcgctggagc aatgaaacgg gccttgggtt agatcgatat gttcgcagct tatgccgtca
      721 aatcggcgtt agcgggaaga accagggaat gacgaagctg gccaccaccc ataacagcag
      781 gttgagcgga atgccgacct tcataaagtc ggtgaagcga tagccgccgg cgttgtagac
      841 gaaggtgttg gtctgatagc cgatgggtgt ggcgaagctg gcgctggcgg cgaacatcac
      901 cgcgaccaca aaagcgcgcg ggtcgacgcc caggtgctgg gccataccaa tggcaatcgg
      961 cgtcaccagc acggccacgg cgttgttcga cagcacctcg gtaaggatcg aggtaaacag
     1021 gtagataaac gacaacatca gcagcggccc ggcccagggc atcagaccca tgacattgct
     1081 gaccatcagg ctgactaggc cgactttgtc catggcgatg ctgatggcca gcatgccaaa
     1141 gatcaggctg aggatcttcc agtccaccgc cttgtaggcg tcttccacat ccaggcagcg
     1201 ggtcgccagt acggtgactg cgccgatgat cgccaggcct tctatgggca tcacgccgaa
     1261 ggcggcgagg atcatcacgg cgagggtggc gatgatcgca atcggtgcct tgtcgcggcg
     1321 gaaggcgcgc tcttgcacgg cgttgaggct gatcagctcg ccgttgtcgg cgaaacgctt
     1381 gatctgtgtc ggcgtgccct ctaccagcat tacgtcgccg aactgcagct cgaagtcatc
     1441 caggttgccc tggatattct cgtcctggcg gtgcaccgcg agcacagcga tgccatagcg
     1501 tgcggtcagg tcgaggtcgc gcatcggccg gtggctgtag cgcgagttac gcccgacgat
     1561 ggcctcggca aggatcacgt catggctgct gatggtttcg aaggcatcgc tgcggttgaa
     1621 gatcaggtgg ccgctctcgc gcagttccac cacatccttg acctggccgt gcaacaccag
     1681 ttggtcacca gcgagcagca gggtgtcgtg ggccggctcg gtgagctcct gctcgtcgcg
     1741 gaagatcttc aacacctgca agccgctgcc gccattgagg ttggcttcgt gcagggattt
     1801 gccgatcact ggcgagtcat gtggcaccaa cagctcggtc ataaacgtgc ggtccagatc
     1861 cgggcgcagt tgcttggaca gagtctcgcg ctcgggcagc aggtgacggc cgatggtcag
     1921 caggtagagc atgcccaccg tcgccaggat caaccccgcg ccggtaattt caaacatgcc
     1981 aaagggctca aggccagctt tgcgcgccac gccgtcgacc agaatattgg tcgaggtgcc
     2041 aatcatggtc agggtgccgc cgagaatggt ggcataggac agtggaatca gcagtttcga
     2101 cggcagcgtt ccggcgcggc gcgccaggga aatcgccacc ggggtaagaa tggccaccac
     2161 tggggtgttg ttgaggaagg cggaaatcac cagggcggtt atggtcagcc cggtgagtac
     2221 gcgaatcggg ctggtgccga ccagatcgcc cagccagttg cccagcgcgt caatgcaacc
     2281 ggtgcgctcc agcgcagcgg acagcacgaa catgcaggcg attgtcactg gcgccgagtt
     2341 gctcagcacg ctgagtactt ccccaggcgt cagcagctga gtaatcagca gcgccgccac
     2401 ggcgatggcc gcgacgatgt ccgggctcca cttctcgcgc acaaaggcgt acagcaccca
     2461 gaccagcagg gcgccgacaa acagcagggg cagaggaaga gagtcccaga acatgaacat
     2521 ccttagcgtg cgaatctcgg gtgaggtatc gggcgcgcca atccagacgg ggtggccacg
     2581 caaggcggag aagataaggg ttgctgctta gagagtctaa gaatgtttgg aaaggtttat
     2641 atgccttttc gatttaatag ataaggctgt actgaccgct ctgccgagca ctgtgcctgt
     2701 gcgccgggtc ggtggcggat taggctcaag gctttagcga gtgggaggtg atatgcgtca
     2761 gctaggcgtt ctgggtggca tgagttggga gtccacggcc agttattacc gtttgctcaa
     2821 tcagggcgtg cgcgaacgcc ttggtggttt gcactcagcc cccatgctgc tgcattccgt
     2881 ggattttgcc gagatcgccg agctacagcg gcgtggcgac tggcaggccg caggcgaact
     2941 attggccaag gccgcacaag gcttgcagca ggctggggca acggccttgt tgctggccac
     3001 taacaccatg cacaaggtgg cgccggccat cgaagccgcg gtggatattc ccttgttgca
     3061 tattggcgat gccgtgggcc aggcgctgca acgtcaaggt gtgcgccgcg cggctttgct
     3121 cggtacgcgc tttaccatgc aggaggattt ctaccgtcaa cgcctggccg agcgcttcgc
     3181 gattgaggtg ataaccccgg acgcggcgca gatggcggaa gtcgaccggg tgatctttgc
     3241 cgagctgtgc cagggccagt ttctgccgca ggcgcaggcg ttctatctgg actgtttgcg
     3301 cacgctgcag gagcagggcg ccgaagtggc gattctcggc tgcaccgaga ttggtttgct
     3361 gctggatggc ctggacgcgc cgttgccgtt gctcgacagc accgagctgc atgtggcgat
     3421 ggggctggag tggttgctgg gctcgtaggg tgttcatcgt gtgggaggcg ctttagcggc
     3481 gattgtcgat gctaaagcgc ctcccacgtc tggctacgat gagcagctga tttccaggtg
     3541 cttgccccac tccggtggac gctgggcgta gcgttcgaag ccgggctgtt catcgaaggg
     3601 ttgcgacagc acctgatgca gttcgcgcac cggggcgtag tcgccctgtt cggcggcttc
     3661 gatcacctgc tgcgccaggt aattacgcaa aatgtacttg gggttggccg cgtgcatgcg
     3721 cgtgcgccgt tttgcttggt catcgccatc ctgggcgctg cgcgccaggt agtcggcagc
     3781 ccaggtgtcg aagccggcca ggtcggtaaa gtcatcgcgc aggcgcgcca ccgcttgcgc
     3841 agccgggctg tcgccgagtt cgcggaagaa caaggtgtaa tccactgcgc tgctttgcat
     3901 cagttgcagc aggcgctgca ccagggcttc gtcgccgtca tcggcgctaa gcaggcccag
     3961 gcgtttgcgc atcaggtcga ggtaatgcgc ctggtacagc ggcagaaaca ggccgagggt
     4021 ctcacgcagc gcttctacat cgaccagcgg caccagggcc tggccgaggg cagcgaggtt
     4081 ccactgggcg atcggcactt ggttgctgaa cgagtaacgg ccggtgtcat cggaatggtt
     4141 gcagatatgt ttggcgtcga aatcatcgag gaaggcgtag gggccgtaat cgaaggtgat
     4201 gccaaggatc gacatattgt cggtgttcat caccccatga cagaagccgt aggcctgcca
     4261 gtaggcgatc atcgccgcgt tgcgttcgat gatttcgcgg aacatcgcca ggtacggctg
     4321 ctcctgccgc aggcagtcgg cgtagtggca gtgcagcacg tgctcggcca gctgtttgag
     4381 ctgttcatgc tgctgggtgt agtagaaata ttcgaagtgg ccgaaacgca catggctggg
     4441 cgccaggcgc aggaccatgg ccgcgctttc ctgcttctcg cgccacaccg gggtgcttga
     4501 gccggtcacg cacagcgccc gcgagctggg gatgccgagg gcatgcagat gttcgctggc
     4561 gagaaattcg cggatcgatg agcgcagtac cgcgcggccg tcgcccatgc gtgagtaggg
     4621 cgtttgcccg gcgcctttca gatgcaggtc ccagtactcg ctggcgtcgt tgattacctc
     4681 gcccagcagc aggccacggc catcgcccag gcgtgggttg tagccgccaa actggtgccc
     4741 ggaatagacc atggcgcgcg gctcggcggt gctccacagc ttgtgtccgg cgaagatctg
     4801 cgcaaacacc tcgctgtcgg cttcgcttgg ctgcagatcg agcaacgcca tggcggccgg
     4861 gctggcgacg accaggcgcg gctcggcaat cggctcgggc agtacgtggg tagagaacgc
     4921 gtcgccgagg cgggcaaaac ggttgtcgaa ctgcagctcg ataagcgact tcacggtcag
     4981 gctccagccg ggcaggtgca gcccggcagt ggtgaggtgg cggcccgcac cagcgggcct
     5041 ggcggcaagt tactgtgggt cgattgccgg cgccggtgcg gtcggtggca gttgcccctg
     5101 ttgcgcatcg gccgccgcag tggctgcaac catttgcccg ctggtcaggt tgagttcctg
     5161 gccgtgcagg ttcttgatca tcacctcgac ctggttgaag gccatttcta cgttgttttc
     5221 ccggaaggtc agcgcgatac gcgtaaggat ctcgtcggtg gcggcgttgc ggtcgcccag
     5281 ttcgcgcacg tggaaacgca gctcgtagtc gaaggtgctg gcgctgatgg tcaggaagaa
     5341 cagcagcggc tctggatcgc gcagtacgcg cgggttctcg atggcggcct gcatcatcag
     5401 tttgcgcgcc agcggcaggt cggtttcata ggccacaccg atcttcacga tgacccgggt
     5461 aaccgtgtcg ttgagcgacc aattgatcag ctggtcggtg acgaaggtct tgttaggcac
     5521 gatgatttct ttgcggtcga aatcggtgat ggtggtggcg cgaatgcgga tcttgctgac
     5581 cgtgccggac aggttgccga tggtcaccac gtcgccgatg cgcaccgggc gctcgaacag
     5641 gatgatcagg ccggagataa aattggcgaa gatctcctgc aagccgaagc cgaggcccac
     5701 cgacagcgcg gctaccagcc actgcagctt gtcccagctg acgccgaggg tcgacagagt
     5761 ggtgacaaag ccaatggcca cgatcaggta ggacagcagg gtggtggtgg cgtaggcgct
     5821 gccctgggcc agcttcagcc gtgacagcac cagcacttca agcaggccgg gcaggttgcg
     5881 ccccagggcg atggtgatgc cgatgatgac caaggcgccg agcacgtcac tgaggctgat
     5941 tggcaccagg gtagcggcat cgccagtgcc gctgctgtat tcatacaggg cgatgttgtc
     6001 gaggtaggcg aacactgaaa tcaggtctgc ccagacccag tacaggcccg cgataaaggt
     6061 accgagcagg gccaggcgga tcagtcgcaa cgactgctgg ttgacctgtt cgatatccag
     6121 ggtcggctcc tcgaccacgg tttccgtacc ttcgccgctg tctttgctct gcgcctggcg
     6181 tttggtcgtg gcgcgctggt aggccaggcg gcgtgccgca acgcccaggc cacggataaa
     6241 ggtggcttca atcaccagcc agaacgccag caggtacagg gtgttgatca ggcgatcgct
     6301 gagtttcagc gcggtgtagt agtagccgag gccgaccgcg acgatcagcg ccagcggcag
     6361 caggctcagg gccatgccga tcagcatgcg gaaggccgag gcatgttcac gggccgggcc
     6421 ggtgagcagc agtttgtgca gcagccagac catcagcgcg tagcaggtca gcaccacggc
     6481 caggccgatc acgtcatcac ccaggccagc cggctggtgt ttggccaccg tgacgacggc
     6541 caccatggcc atgacgacca ggccaagacg gcgcacctgg cgatgcagga aggccacctg
     6601 cgagcggccc cagcgaaagt gcagctcggc cacgccgcca tcggcgagga tgcgatacag
     6661 ggtatagaac accagccagg cctgggccat ctgaaacagc gcctcaccca ggttggcgtt
     6721 ctgtccgcgg gcgtccatct gcagggcaaa gccgcacagc gccaggaaca gcgtgccggg
     6781 tagcgccagc agtacgttga gcatgattgc catcggcgtg tgcagctgac tgtcacgttt
     6841 gaaatggccg atgtcctggt gtagctctgc gagcttgcgg tacaggtagc cgcggcgcca
     6901 cagcagtacg ccaatcagca acagtaatgg caaaaacagc agcggccgct cggtcaggcc
     6961 ggcacccagc tgtcgcacgt tggcgcccca gggcagttcg gcaatctgcc gctccagcag
     7021 gcgcgggacg gatttgaacc actccagatc cagcggcttg ttgctgggga tccagaacat
     7081 ctgctcgtcg agggttgcgc gcagggtgtc ggcggtggtt ttcagctgct tctgattcag
     7141 ctgcaaggtg atcgactcgt tgagcagggc gttcagctca cggttgaggc ggtcgatcag
     7201 ctcacggcgg gtgttgatca tttcaagcag atcggtgcgc agggcctggt tattgggctc
     7261 attggcctgt tcgctgagca gggtgtcgac atacacgctg gggttgttga tggcttcgcg
     7321 ctgctggttg agctcgaact ggtacagacg caggtcggct atctggtctg gcaggctgct
     7381 gtcgagcttc agtgttggca gggcctgctg ctgcttataa aggatgcgcg aaagcaacag
     7441 gctgccctgc agcacgctga tctgctcttc cagggcctgg tcgctctggc tcaggctgtc
     7501 cagttgctgc ttggtttgca ggttcaggcg ggtcagttca ttgagccggt cggtggcgcg
     7561 cagcaggtag tcggagagct ttaggttgag cgcgctttcc tgcgcgagca ggctgtcggt
     7621 accggccttt tcgacttcgc gggattgttc ggcgagggtc tgctcggaga ggtcgcggcg
     7681 tttttcattg atcaggttct gcaggtcgag cagctcctgt tcgaggcgtt tgatgcgctc
     7741 ttccaaaagg ctgcggcggc tgttgcctag gtcctgcagc aggctgttgc cggccagttc
     7801 ctggcggcgc agttgggttt gtgcatcgag cttggccagt tcagcattca gctggttacg
     7861 ccgctcgttg ctcagcggtt tgctggcatc ccggccactt ttcaggatgt tgttgatttc
     7921 ctggctacgg gtctggttgc cgctgatttc tgcttgggcg cgttctgggc gggtttgcgc
     7981 ggtgatcacc aggctgttgg cgtcgatgat ctgcttctgc cactcgttga gttgcgcgtt
     8041 gcggtcggcc agcagttgtt cgagctgcgg caacgcgctt ttgccgtagc gtagattgac
     8101 ctgggtgggt gtgctgtttt taagccgctc gtaatcgcgc tgcgcctcgc caatcaggcg
     8161 tggggcctgc tgcagctgct tctcaaggtc ggcccggcgc tgctcgctgt cggctttctc
     8221 gtcgagcagc gccagggttt tctccagggt ctgcttgacg accagttgct cggcttccgg
     8281 cagcttgcga tcggccaggt tgtccaggct gctttgtacc tcatcgcggc tcggcggctc
     8341 ggcggcatgt agcaaggggg cgctcaggca cagcccgagc agggcaacgg caagataagt
     8401 gcgaagagaa ggcatagtga cgatcgttgg ctcgaaaaga agctgagttt agaggaagag
     8461 cccaggtcct gggcgactgc cttcggggaa tctgacgccg accttgcgga tcttgttccc
     8521 ttccatggcc gccacggtcc aggtcaggcc ttgccattcc acctggtcgc cgattaccgg
     8581 cgagccgcca atcttgtgcg cgataaagcg gcccaggtgc agctggcctt cggcgccttc
     8641 caatttcagg ccatagagtg cggcgatggc ggccagctct gcatcgcctt cgagcacgaa
     8701 atcaccgaag aaacgcaggt cctgcccgcg cgttggcgcc tggctgaaca gcttgccgag
     8761 agcgggcagg tcgtgttcat ggccgaccac gcagagcagg tctccggcat ccagggtggt
     8821 gctgccggag gggtgtagca gctccttgcc acggaacagc gcggcgatgc gcgtgccatc
     8881 aggcatcttc agctcgcgca gcggcgcgcc gatgcaccac ttttccgcac cgagacggta
     8941 gataaacagc tcccactggc tggtcgggtg tacgtccagc ccggcgcgcg acactggcgc
     9001 gggctccggc ggtacggtga cgcgcagcaa tttggccgca aacggcaggc tggtgccctg
     9061 cagcagcagc gagaccagca cgataaagaa cgccacgttg aagaacagct gggcattcgg
     9121 caggccggcc atcagtggga acaccgcaag aataatcggc accgcgccgc gcaggcccac
     9181 ccaggcgata aaggcgcgct cgcggtcatg gaaccccttg aacggcagca ggccaatgaa
     9241 gatcgacagc ggtcgggcaa agagaatcat ccacagcgcc aggcccaggg ccggtaaggc
     9301 aatcggcagc agctcgtgcg gggtgaccag caggccgagg accaggaaca tgccgatctg
     9361 cgccagccag gtcaggccgt cgagcatatg caggatgccg tggcgcgagc ggatcggccg
     9421 gttgcccagc agtaggccgc acaggtagat cgacaggatg ccgctaccac cgacggcggt
     9481 ggtgatggcg aagatgatca gaccgccgct gaccaccagc aggggataca gaccgttggc
     9541 cagttccatg cggttgatca ggttgagcag cagccagccg ccacccaagc cgaacagagc
     9601 gccgatgccg aactgttgca acagctgcac cagcaggttc cagctcatgc cgtcctggcc
     9661 gctggcgagc atggcgatca gggtgacggt aaggaacacc gccatagggt cgttgctgcc
     9721 ggactcgatt tccagggtgg cgctgacccg ctcgttaagg ccacgcccgc cgagcaggct
     9781 gaataccgca gccgcatcgg tggagccgac gatggcgccg atcagcaggc cttccatcca
     9841 ggtcaggtcg aacaaccatg ccgccgcgac gccggtcagg ccggcagtga tcaggacgcc
     9901 gacggtggcc agagagaggg acggccacaa ggccacgcga aagctggata cccgcgtacg
     9961 catgccgcca tcgagcagga tgatggccaa cgccaagttg ccgaccagat aggccagcgg
    10021 gtagttgttg aagatgatgc cgccaggacc atcagtgccg gcgagcatgc cgacagcgag
    10081 aaagatcacc agaatcggga tgccgaagcg cgagccgaat gcactgacca gaatgctcat
    10141 tgcgaccaga acgccaccga tcaaaaacag attattgatg gtgctggcat ccaaggcgcg
    10201 tactccgtgc gggttggtga gcgtgccact atgcatggcg cgtgccagag attctaaccg
    10261 ctgccttcac actgcgctca aaaaaaacgc cccgcgttca gcggggcgtc aggattacgg
    10321 atacgcgagc gggatcagaa ccaagcgtcc tgcatgccca gacaggtgtc gtcgcgggct
    10381 tcgaggatgg ccaggtcgtg gtggcagctc ggcacttccc aggtcaggaa gtagcgcgcg
    10441 gcctgcagct ttccctggta gaagtcggca tccgcagggt taccgcgcgc caggccttct
    10501 tcggcgcgga tcgcctgttc cagccagcgc cagccaatca cggtatggcc gaacaccttg
    10561 aggtacagcg ccgagttggc cagaccttcg ttgaccttgc cgctcatcaa atcgcccagc
    10621 agggccagag taacggtttg caggcgtgcg agtagttgct ccagcggctg gcgcagggcg
    10681 gtcagcgagg catgggcttc ggcgcgctgg caggtgtcgt ggatcagctt gagcagctgc
    10741 ttgagcgccg cgccgccgtt catcgagacc ttgcgcccga gcaggtcaag ggactggatg
    10801 ccgtgggtgc cttcgtggat cggattcagg cggttgtcgc ggtagtactg ctccaccggg
    10861 taatcgcggg tgtagccatg gccgccgaga atctggatcg ccagctcgtt ggccttgagg
    10921 cagaactccg acggccagga tttgacgatc ggggtgagca gatcgagcag ttccagggcg
    10981 atccggcgtt cgtcttcagt cgccaaagtc tgggtgtcgt cgaacagccg cgcggcgtac
    11041 aggccgaggt cgaaagcgcc ttctacatag gctttctggg tcagcagcat gcgccgtaca
    11101 tcggcgtgtt cgatgatcgc cacctggccg gaggttgggt ctttgccgtc gggcaggcgg
    11161 ccttgcgggc gctggcgggc gtagtcgagc gaatacaggt agccggcgta gccaagcatc
    11221 accgcgccca tgccgacgcc aatacgcgcc tcgttcatca tctggaacat gtaggccagg
    11281 ccatggtgcg gtttgccgac cagatagccg gcgcaatcac cgttatcgcc gaagttcagt
    11341 gcggtagagg tggtgccgcg ccagcccatc ttgtggaaca gcccagccag caccacatcg
    11401 ttgcgcttgc ccaggctgcc gtcatcgtta accagaaact tcggcacgat aaacaggctg
    11461 atgcctttca ccccggcggg ggcgtccggc agcttggcca ggaccatatg cacgatgttt
    11521 tccgacagcg ggtggtcgcc accggagata aaaatcttgt tgcccttgag ccgataagta
    11581 ccgtcgcctg ccggttcggc gcgggtgcgg atatccgaca gcgaggagcc agcatggggc
    11641 tcggtcagcg ccatggtgcc gaagaaacgc ccgtcgatca tcggctgcag aaagcgctgc
    11701 ttctgctcat cactgccgaa ggtctcgatc aggttggccg cgcccatggt cagaaacggg
    11761 taggaggtgg tcgccgcgtt ggccgactgg aagtgcgcga agcaggcctg cgacagcagg
    11821 ttgggcagct gcataccgcc gacctcgaac tcgcgggtgg cattcaggaa gcccgcttcg
    11881 aggaaggcgt ccaccgccgg tttgacctcg gggatcagca ccgcggcgcc gtcgacatac
    11941 tccggctcgt gttcgtcatt cttgcggttg tgcggggcga acaggttttc cgcgatgctg
    12001 cgggcggtgt cgatagccgc atcaaaggtt tcgcggctgt gttcagcgaa acgttcacgc
    12061 tgggtcaggc cttcggcgtc cagcacttcg tacagctcga aggccaggtt gcgggaactg
    12121 agcagggact cggacatggg tgtacctccg gttgcgatgc gccgagtcta agcagccgcg
    12181 agtgcggctc ccagcactat caataagcgt gatagaggct tagctagcaa agccacgggc
    12241 cagttcggct gtcagctcgg cggcaccgat gctcttgcaa ccgctgacat tctgcccggc
    12301 ccagagtggc gaaaagtcac cactgccctg gctttccgcc tggctgcgta gcggcgcaat
    12361 ggctgctgtc gccagaggga aggccggcgc cagtgggctg attggcccca gctcgcgcat
    12421 caggcgattg acgatgccgc gcgccggccg gccgctaaac agattggtca gggccgtatg
    12481 ttgcgccgcc tggctatgca gggcggcgcg gtggatggcg ctggtgctgg cctcgtcgca
    12541 acacagatag gcggtgccga tctgcacgcc tgcagcccca agggccatag ccgcgcgtac
    12601 accggcggca tcggcaatac cgccggcggc gatcactggc agctgcacgg cctggacgat
    12661 ctgcggcagt agggcaaagg tgccgagctg gctgttcagt tcatcggtga gaaacatgcc
    12721 gcgatggccg ccggcttcca ggccctgggc gatgatcgca tcggcgccgt tggcttgcag
    12781 ccacagcgct tcctccaccg tggtcgccga gctaagcacc ttggctccgc aggctttaac
    12841 ccgtaccagc agctcggcgg agggtaggcc gaagtgaaaa ctcaccaccg ccgggcgaaa
    12901 ctcatcgagc agatcggcca tttcggcgct gaacggcagg cgaccggggc cgctggcgat
    12961 agtcgccggg ttgatgccta gctcggtgta gaaagggctt agcgcctcgc gccaggttgc
    13021 ttcgcgagca gagtcgatgg ctggcggggt gtggcagaag aagttgaggt tatagggctt
    13081 gctggttttc tcgcccagcg cctgcagctc tttgcgcaac ccggccgcat cgagcatggc
    13141 gcagggcagc gaacccagcg cgccggcatt cgacacggca gcggccaagc catgcagctg
    13201 cacaccagcc attggtgcct gaatcagcgg tagctgggtg cctaatagcg cttgcagtga
    13261 catggtggtt ctcctggcca gacggatcag tggccaccgt actgatccat ctggcgcatg
    13321 gcaaatgccg ccaccctgtt aaactgcccg gccacaggag cccgcccaaa tgaactaccg
    13381 ccacgccttc catgccggca accatgccga tgtgctgaaa cactatgtct tgagccgtct
    13441 gattgccctg ctgtcacgca aggaggcgcc gtttgcctac ctcgacagcc atgctggcat
    13501 cggcctgtat gacctgcgcg gcgatcaggc cagccgcacc ggcgaatggt tggaaggcat
    13561 cgcccggctg tggcaggcca cggatgtacc tgacccgttg ttggactatc tggaagtgat
    13621 ccgtgcgatg aatccggatg gcgagctgcg ccattacccc ggctcgccgg agctggcgcg
    13681 cctgctgacc cgcgaacaag accgcctgca cctgaacgag aagcacccgg aagacggtcg
    13741 cctgctcaag gaaaacatgc acggcgaccg ccgtgtggcc gtgcacctgg gtgaaggctg
    13801 gtatgtgcca cgcgccctgc tgccgacccg cgagaagcgc gcgctgctgt tgatcgatcc
    13861 gccgttcgag caggtcgatg agatggagcg ctgcgcccag tcgctgagcg aagccatcgg
    13921 ccgcatgcgc caggcggtgg tagcgatctg gtacccgatc aaggacctgc gccagctgcg
    13981 gcgtttctac agcgacctgc aaaagagcaa ggcgcccaag ctgctgcgca tcgagctcta
    14041 tgtgcactcc accgatgaag ccgcgcgcct caacggctcc ggcctggtga tcagcaaccc
    14101 gccatggggc ctggaagacg aactcaagca gatcctgccg tggctggccg agctgctcgg
    14161 gcagagccag ggcggctggc gcatggattg gctgattgag gaaagcagcg cgagctagcg
    14221 acctttgctc ggctcgacaa agtaactgag ctgctggcgc gggttaaggt attccagcgc
    14281 ttcgcgcggt agctgagacg ggcgcaggct gctggcgatg ctcaggcgcg gttcctgttc
    14341 cagatcgatg atcagggcct ctgccttggg cactgcggcg tcgtagacaa tcagcgtcgg
    14401 tttcagcggc tggctggggt tcatccccag gaccaagcca tagcgctgat cgtccagctg
    14461 caccaggcag cctggcgggt agatgcccat gcagcgaata aaagctttca gtgccaccgc
    14521 atcgaagcgc gcattgtatt gcttgaacat cagcgccagg gcttcgtggg ggctcaggcc
    14581 atcacgcggg ttgagcgggt tacagaggtt gtcgaagtgg ttggtgatgc acaccaggcg
    14641 gcttagccgg ccgatcgcgg cttcacgcag ctggcgtggg tagccgctgc cgtcgcagta
    14701 ctcgtggtgc tcgttgatga tgcgcaacac ctcgtcgtcg agcatcagct tgtggcccat
    14761 gcgcaggccg aactcgcagt gcagttgcag cagttgctgc tcggggcggg tcagcggttc
    14821 gcttttcagc agcaccttgc tcggcacttc cagcttaccg acatcatgca gcagcgcgcc
    14881 gaggccgagg atatggcagt cctcgctgtc caggccgagc tgacgaccga gcatcaggct
    14941 cagcaccgtg acattcagcg agtgcaagta gctgtcttca gcggtcttgc tattgatact
    15001 gtgcagcacc acgccgctgc cgccgagcag ggtctcgacc atcgcgccga caatctcgcc
    15061 ggcctgtttg atggcgtctt ccgggcggct gcgcagggtc tggttaagcg tcttgacctg
    15121 ctggctggcc tggacgaact tgcggtcaac ctcggccagg cgtttgcgca gggccttgag
    15181 cttttccgcg cgggccaggc tggcctgctg ttgcgagctg agcgttggcg cgactacgga
    15241 gtcggccgca accggtgccg ggcttgcgct tagtggcggg caagtgctgc gcgccgggtc
    15301 atagcgcagg cgctcaaggc cgagtgctcg cagggcgctg atctgcgctt catccttgat
    15361 cttgaagttg ctgaaggcaa agttgtgttc ccaccaggcc agttcgagct ggatatacag
    15421 gccgacgcac agctgatcgg gggatatgta gacagaggct gcaggcatgc gggtatcggc
    15481 aatgatggct attggctggt agtctagcgg tttgcctatg agctagcagt agccggcgat
    15541 aagttctgta cctaggtttg gcgtctagcc cactaaacca tcacatcgac actattgccc
    15601 agattggacg ggttgctcgg cgcgctggct ggcgtggcgg cctgcaccag gccgccgatg
    15661 ctggcagcct gcaattccaa agttttgcgc agcaacagca gtgaagccag ggctgaggct
    15721 tgagccgctg cttgggtgga cacctgattg gtcaggctgc tgaggtccat ggtgatattc
    15781 cctctgcttg cgggcctatt tagcccggca ggcaggctcc tgttccaccc aggccgcaat
    15841 agccgcccgg attttttgcc aggtactgct ggtgataggc ctcagcgtag tagaagggcg
    15901 gcgcctcaca tacttcggtg gtgatggcgc catagccggc tttgctcaat tcggcctgga
    15961 actgcgcctg gctgctctgc gcggcggtca gttgctcggg gccgtagcaa tagattgctg
    16021 aacggtactg gctgccgata tcgttgccct gacgcatgcc ctgggtcggg ttgtgcgcct
    16081 cccagaatac tttcagcagt gcggaatagc tggtgacctt tggatcgaac accaccagca
    16141 ccacttcggt gtggccggtc aggcctgagc aggtttcgtc ataggttgga ttcggcgtat
    16201 ggccaccggc ataaccgacc gccgtactcc atacgcctgg ctgctgccag aagcgccgct
    16261 cggcgcccca gaaacagccc atggcgaata ccgcctgttc aaggcccgca ggaaatggcc
    16321 cctgcagtgc attgccattc acgaagtgcg cggcgggcac cgtcatgacg tccgcgcggc
    16381 cgggcagggc ttgctcggcg gtgggcagtt cgagtttgtg ggcgagaatt tgcgagcgca
    16441 gaaccatgag ccatttcctc agtagaagca tgtgtgtcat agaccagcaa tgagcgacct
    16501 tgttacagcc ggctagcctc ggtttggata acgcttgagg cttttcaaca ggtcgcggct
    16561 ggcgatgggc ttgtcgaaca ggtagccctg accgatatcg cattgctggc ggcgaagaaa
    16621 gttgagctgc gcgactgtct cgatgccttc ggctaccacc ttgagtttca ggttatgcgc
    16681 catggcgatc accgcagagg tgatttccat gtcgtcctgg ttctccggga tgtccttgat
    16741 aaaactgcga tcaatcttga tcacgtcgag ggggaatttc ttcaggtagc tgagtgagga
    16801 gtaaccggtg ccgaagtcgt ccatggccag ggtcaggccc aggctcttca ggtggttgag
    16861 ctggtggcgg gtgtcgtctg tggcttccag cagcaggctt tcggtcagct ccagctcgag
    16921 cagggccggg tcgagttgtt cttcatgcag gatggcagcg acctcgccag cgaggtccgg
    16981 gtcggagaac tgcttgggcg acaggttgat cgccatctgc agcggtgtgt agccgacggc
    17041 gatcaactgc ttgctcatgc ggcaggcctg gcgcgtgacc catttgccga tggggatgat
    17101 caggccggtt tcttccgcca cgctgatgaa tttgtccggg ctgatcatgc ccttttccgg
    17161 gtggtgccag cgcagtagcg cttcgagccc cagcaaccgg ccgctcttca ggcacagctt
    17221 gggctggtag aagacttcta gctcgttctg catcagcgcg cggcgcaggt tgctctcgac
    17281 gaacagcttg tagtgcgcct cggcattcag cacatcggta aacacctgca gctgattttt
    17341 gccgttggcc ttggctttgt gtagggccag gccggtgtgc ttcattaagg tctgcgggtc
    17401 gtcgccatgt tcgggtgcgc agcccaggcc gatggagccg gtgacgctga tcaactggtt
    17461 gtcgacgaac aatggcttgt ccagggtgtg cagcaacttc tgcgccacca gttggctgtc
    17521 agcctgattg gcgtcaagca gtacggcgaa ttcgttgctg gcaaagcgcg ccagtacgct
    17581 gttggggtgc agggtgttgc gcaagcggcg cgccaagctg actagcagtt tgtcgccggt
    17641 ctggtggccg aggctgtcgt tgatccgctt gaagttatcg atatccacca gcagtaggct
    17701 gatcggtttg cccgggtgtt cgctgaagtg gcgctccagc gagcgaataa aggcgtagcg
    17761 gttgcccagc ccagtgaggt tgtcgcgaaa ggccaggcgc tcgatatgct gctgcgcttg
    17821 cttgctcgca gtaacgtctt cgtagatgcc aatatagtgg gtcaggcagc catcgtcggc
    17881 gtgtaccttg gacagcgaca actgccccca gtaggggtcc aggttcttcc ggcggctctt
    17941 gaattcaccc tgccagctgt tgtgttcgac caggccggaa tgggtgtcga acagcagatc
    18001 gctgaggttt tccagagcgg ggatttcgga cagacggcgc ccctgcactt cgccagcgct
    18061 gtagagggtg atggcggtaa aactcgggtt gacgtactcc acgaggccgt cgcggtctag
    18121 caggataaag gcgctggagc tctgctcgac agcgcgctgg aacaggtata gggcattggt
    18181 tgcagagcgt cgttgctggt tggccaacac ctgggcgtac tggtcagcca gctcgccggc
    18241 aaaggcaatt tcgtcggcct gccaggtgcg cggcgcgccg atatgttcca gacacaacac
    18301 gcccaccacc tcgccggcga tgcggatgct ggcatcgagc atggcggtga tgcccatggg
    18361 ttctaggtag ctgctgctca gctcgcaggt gcgtgggtcc tggtgggcgt catgcgcgtc
    18421 cagggcgcgg ccgctgtgca gtgcctgcag gtaggcgggg taggggctgg catcgatatc
    18481 ggcctggtct atatgccggc cttggtcctg ttggtacagg gccaccgact gcagcacatg
    18541 ctgattgagg ttccaaatac ctacgcggct gactccatac agctcgcagg cgcttttgct
    18601 gattagctcg gcggcttcgc gcaagggatc attgtcgccg taatggtggc gcgcaagggt
    18661 gacgatcagc tgtcgctggg cttgcctacg ttgctgctgc gccagcggct cggcctggct
    18721 gtgctcatac agctccaggg gctgtggcga aggtgcgctc tgctcggtta tcaccagcag
    18781 ataaccgcgc agcaggtgtc ggttgtgctg cttgaaggct tcgcccactt ccatcagcgt
    18841 caggctgccc agcggtgtgt gcaggcgata ctgcacgctg taatgccggc gctcggcaag
    18901 ctgttgcgca atgctgtcgt gcaactggta gcgtgccgtg ggttccatca ggctggcgta
    18961 aggggcatcg agcagggagc agagttcctg agctggcagg ccgaactgct gttcgcagcc
    19021 tgagtcgagg tacagcaggg tccagctggc ttcattcaag cgctcgaaac gcagcaagcc
    19081 aaggcgcgaa ggcactggca actgcgtcac aacctcggcc gctatacgac ttgcggcatc
    19141 aggatggctt ttcattcggt ggctggcttc cagtatgctg atcgacgcgg caatcgactc
    19201 acttcattta cagcgtcggg taagggtgca tcatgcaatt gcgactgaca agaaaactaa
    19261 tatccgcagt gctgctgagc agctgtatgg ccaccccaac ggtattcgcc gctgagcctg
    19321 acacgggttt gagcagtgcc gaacagggca attatctgct cgaactgaaa cgcctttatc
    19381 tgtccccaaa tgaccgccaa gcgctgttgg cccactgcaa tgatctgctg aaaacctacg
    19441 ccctgcgcgc cgcctatcag gtcggtcagg cgcagcgcca ggacctgatt tatcagctgc
    19501 gtcagggcga gtccggcgag ctgttgctgc gtgaagaaac ccgtggccag cagggcaccg
    19561 atattgccgt gcgcaaccag cgcgtaccgc tgtttggcgt cgaccccttc gtgcgttacg
    19621 agtgcccggc caacggcatt agctgcgtac tgcacaaccc taacgacggc agcccaatgc
    19681 tgaccatcgt gcgcgatcac aagggcgctg cggaactggc caaggccctg agttttctga
    19741 ttcgtaatct gcagaaaggc tgatcgcctc ccacattttc tgtctttgcc ataaagctaa
    19801 aaaaaacccc gcctaaaggg gcggggtcga ggatacgagc gtggctgctc gaaagttgat
    19861 gcgttacagc agcatggtgc ggatgtccgc cagtacgtca cccaggcgct tggtaaagcg
    19921 cgccgcagcg gcgccgttga tcacccggtg gtcgtaggac agcgacagcg gcagcatcag
    19981 cttgggctgg aaggctttgc catcccaaac cggctggatg gtcgccttgc tgacaccgag
    20041 gatcgccacc tccggcgcgt tgacgatcgg cgtgaagccg gtgccgccaa tgtgaccgag
    20101 gctggagata gtgaagcagg cgccctgcat atcatcagcc gacagctttt tgctgcgcgc
    20161 cttttccgct agtacggcgg cttcggcagc gagctgcagc aggctcttct gatcgacgtt
    20221 cttgatcacc ggtaccagca ggccatccgg ggtgtccacg gcaaagccaa tgtgcacgta
    20281 tttcttgcgg atgatggctt tgccgctcgg cgccagtgag ctgttgaagt ccggcagttc
    20341 cttgagcagg aaggcgcagg ccttgagcag cagcggcagc acggtgagct tgaccccagc
    20401 cttttccgcc acagctttct gcgcgacgcg gaaggcttcc agctcggtga tatcagccga
    20461 gtcgaactgg gtcacgtgcg ggacgttgag ccagctgcga tgcaggttgt tggcgccgac
    20521 ctgcatcagg cgggtcatcg ccacttcttc gatttcacca aacttgctga agtccaccac
    20581 cgggatcggt gggatgccgg cgccgccggt tgcgccggca gccggggctt ccttggcctt
    20641 ctgcatcatc gctttgacgt aagcctgcac gtcctctttg agcacacggc ctttcggccc
    20701 ggtgcccggt acggcggaca gctcgacacc aaactcgcgc gccagcaggc gcacagccgg
    20761 gcccgcatgc accttggcgc cgtcgcgctt aggggcggcg gcaacgttgg cagcagcggc
    20821 tgccaacgag gcaattgcac ccacttcggc agcgaccgcc ggatgcgcac cggccggaac
    20881 cttgtgtacg tcactagcgg gtgcagcggc cggtgctggc gctgctgctg gaaccgcagc
    20941 ggcgccggcg actttcagct tgaggatgaa atcacccgtg ccgacttcgt cttccagttt
    21001 gacgctgatg ctttccacca cgccggcagc cggcgagggg atttccatgc tggccttgtc
    21061 cgactccaga gtgatcagcg attggtcggc ttcgaccgta tcgccgacct tgatcaggat
    21121 ttcgatgacc ttggctttac cgtccgaacc aatatccggc acatgcacgt cttgcacgct
    21181 gctggtctgc gcggctggtg ctggtgctgg tgctggtgct ggtgctgcgg cagcttcagc
    21241 cgcagcggct ttgggcgcct cagcaggtgc tgaaactgga gctgcgggtg cttgggcagc
    21301 gccttcggct tccagctcaa acagttcgtc gccttctttc aggcggtcgc ccagcttcac
    21361 tttcacgctt ttcaccacgc cgcttttcgg cgcggggatt tccatgctgg ccttgtccga
    21421 ctccagggtc agcacgctct gatcggcctc gatgcgatca ccgaccttga ccatcagttc
    21481 gattacttca ccctcaccac caccgatgtc gggtacgcga atcaattcac tcatcgtttc
    21541 gctccttagc agtccagtgg gttgagtttt tccgggttga tgccgaact
//
LOCUS       DASRUM010000051        61286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1701755, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000051 DASRUM010000000
VERSION     DASRUM010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 61286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61286
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701755"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..712
                     /locus_tag="VFF22_03675"
     CDS             26..712
                     /locus_tag="VFF22_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HZX15953.1"
                     /translation="MSTIAENIAKVGVRIREAAQASQRDCATVGLLAVSKTKPAAAIR
                     QAFAAGTRDFGENYLQEALEKQVELSDLPLTWHFIGPIQSNKTKPIAEHFAWVHSVDR
                     LKIAQRLSDQRPTHLPALNICLQVNVSGEASKSGCNPDELPGLAQAVTQLPNLRLRGL
                     MTIPEPTDDPSEQRAAFARLRELQQGLNLDLDTLSMGMSHDLEAAIAEGATWVRIGTA
                     LFGARDYSSN"
     gene            748..1566
                     /gene="proC"
                     /locus_tag="VFF22_03680"
     CDS             748..1566
                     /gene="proC"
                     /locus_tag="VFF22_03680"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HZX15954.1"
                     /translation="MTTPRIAFIGAGNMAASLIGGLRAQGIDAGAIRASDRGAEQRSK
                     ISAEHGIETFASNAEAIQGADVIVLSIKPQVMKEVCLDLAAHISGQQLVVSIAAGISC
                     ASLESWLGPRAIVRCMPNTPALLRQGVSGLYGNAHVSQAQRQQAEQLLSAVGLALWLD
                     QEAQIDAVTAVSGSGPAYFFLLIEAMTAAGEKLGLPRETAAQLTLHTALGAARMAVAS
                     DVDASELRRRVTSPAGTTEAAIKTFQADGFEALVEKALNAAAHRSAELAEQLGQ"
     gene            1576..2166
                     /locus_tag="VFF22_03685"
     CDS             1576..2166
                     /locus_tag="VFF22_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HZX15955.1"
                     /translation="MIGLNTAAVYILQTIGSLYLLIVLLRFILQLVRADFYNPVSQFI
                     VRATHPLLKPLRKIIPSLAGLDLASLVLAIVVQLVLMALTLMLLGYGLDNPLQLLVWS
                     IIGVTALFLKVFFFALIISVILSWVAQGSHNPTAMLINQICEPLLSPIRRILPGMGGL
                     DLSPIVAFLLLNLIDMLVIRNLAIMTGMYKGLSLAI"
     gene            2180..2476
                     /locus_tag="VFF22_03690"
     CDS             2180..2476
                     /locus_tag="VFF22_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HZX15956.1"
                     /translation="MSHFRWDGADLILECHLQPKASKDEFAGLHGERLKIRLTAPPVD
                     GKANAHLLAFLASVFAVSKSQVSLESGLQSRQKRVRIKKPQRLPGELALTIRSP"
     gene            2714..4696
                     /locus_tag="VFF22_03695"
     CDS             2714..4696
                     /locus_tag="VFF22_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin-like GTPase family protein"
                     /protein_id="HZX15957.1"
                     /translation="MSMERLSQQVDAYVTWKRELMREITRYRSWLVTNRLNSEAVEAK
                     LERALKLLRTDHITLAFVGEFSRGKTELINSLFFSSYGQRMLPSQAGRTTMCPTELFF
                     DPRSERSYIRLLPIETRVSEASVAQFKRIPRHWVNIPLDLSDPDNMVQAFVQVARTKP
                     MPVEQAIALGFHPDMLESTHKPGEVLVPAWRHAMVNFDHPLLRQGLRILDTPGLNALG
                     SEPELTLSMLPSAQAIIFLLSADTGVTASDMAVWQQHIRQLDEDTQTSLFAVLNKIDV
                     LWDDLAGEAFVQNAIRQIQTSTARQLGIRVEDVLPLSAKQAMLAKVRKDPELLARSQM
                     SNLENLLCERIIAQKERLLEDRVVNQVLALLQNSQHVLNLRLEKVNEQQALLSNHQHD
                     NGQMLFELTAKTKEDHSQHHKRLLGLKTNQRLLKRQGDLLRHASRSERLDEHLNKVRR
                     NLTGSWTTLGINQAILHFFRSVEQDLHNLAQEAEMANKMVAAIYRRHNEENPLHGVDA
                     PQFNVKRYLRELKQQQTKADQFRLQLKTLLTEQRSLTKRFFATLVQEVIGLHQRMRQE
                     AEQWASDALMPLMQHTLEHKQLLETHMLRLKALAQETQQARQRGQQLARYSSELEQQL
                     AQANDMLRILRRPAPVQRQGKVVNLPGVARPHSIGE"
     gene            4952..5569
                     /locus_tag="VFF22_03700"
     CDS             4952..5569
                     /locus_tag="VFF22_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HZX15958.1"
                     /translation="MPEVRPKLLVFAGPNGSGKTSITEMLRKHHWLSDCTFVNPDEIA
                     QNEFGGWNSEDALKQAAEKSERLREQCLIEKRSLVFETVLSTEDKLDFIQRAVDAGFF
                     VRVFFVSTSHPAINAYRITYRYMDGGHEVPISKIVARYTKSIANCVALTSLVDRLYVY
                     DNSVNDQDPRLLFRTREGLLCKTYAADDETPEWALQIRQCIPSGT"
     gene            5637..6776
                     /locus_tag="VFF22_03705"
     CDS             5637..6776
                     /locus_tag="VFF22_03705"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HZX15959.1"
                     /translation="MSTAFPQDSVGLVTPQVLHFAEPLALACGRSLADYQLIFETYGE
                     LNATASNAVLICHALSGHHHAAGYHSAEDRKPGWWDSCIGPGKPIDTNKFFVVSLNNL
                     GGCNGSTGPSSLNPATGKPFGADFPVMTVEDWVHSQARLADNLGIQQWAAVIGGSLGG
                     MQAMQWTISYPERVRHCLAIASAPKLSAQNIAFNEVARQAILTDPQFHGGHFQEQGVI
                     PKRGLMLARMVGHITYLSDDAMGEKFGRGLKSEKLNYDFHSVEFQVESYLRYQGEEFS
                     GRFDANTYLLMTKALDYFDPAASFDGDLAKTLAHVSADFCVMSFTTDWRFSPARSREI
                     VDALIAARKNVCYLEIDAPQGHDAFLIPIPRYLQAFDSYMKRIKV"
     gene            6779..7399
                     /gene="metW"
                     /locus_tag="VFF22_03710"
     CDS             6779..7399
                     /gene="metW"
                     /locus_tag="VFF22_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HZX15960.1"
                     /translation="MRADLEIIQEWIPAGSRVLDLGCGNGELLSWLSEHKGVSGYGLE
                     IDPENIAQCVSKGVNVIEQNLDKGLGNFASNSFDVVVMTQSLQALHYPDKVLAEMLRI
                     GKTCIITFPNFAHWRCRWYLTTKGRMPVSDFLPYTWFNTPNIHFCTFEDFEKLCHDQG
                     AKVMDRLAVDRDHRHGWASRIWPNLLGEIGIYRISGPTAEDVQIAN"
     gene            7432..7851
                     /locus_tag="VFF22_03715"
     CDS             7432..7851
                     /locus_tag="VFF22_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4426 domain-containing protein"
                     /protein_id="HZX15961.1"
                     /translation="MRRIALLFIALCLSLPALAERKQTFGDLDVHYIAFNSGFLQPDI
                     AAASGLVRSKTQGVVNISVLKAGKASSASVSGEVKDLVGRSQPLTFKQVTEGEAIYYL
                     AQFNFTQREMLRFTINVHATDGVAHSFNFNQEVFPDE"
     gene            7865..8461
                     /gene="rdgB"
                     /locus_tag="VFF22_03720"
     CDS             7865..8461
                     /gene="rdgB"
                     /locus_tag="VFF22_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HZX15962.1"
                     /translation="MMHFNELVLASHNAGKLKELQAMLGSSVRVRSVGEFSMVEPEET
                     GLSFIENAILKARNAARLSGLPALADDSGLAVDFLGGAPGIYSARYADGQGDAANNAK
                     LLDALKDVPDAERGAQFVCCLALVRHADDPLPIICEGLWHGSILHAAQGVEGFGYDPL
                     FWVPERDCSSAELPAAEKNQISHRARAMALLKQRLGLA"
     gene            8458..9618
                     /gene="hemW"
                     /locus_tag="VFF22_03725"
     CDS             8458..9618
                     /gene="hemW"
                     /locus_tag="VFF22_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HZX15963.1"
                     /translation="MTTAAGGGFELPPLALYVHIPWCVKKCPYCDFNSHAAGPTLPEE
                     EYVDALLADLDADLAQVHNRPLSSIFFGGGTPSLFSAKALGRLLEGVERRVPFAADIE
                     ITLEANPGTFEQAKFKDYRSLGINRLSIGVQSFQAPKLIALGRIHDGDEAVHAADMAR
                     AAGFDNFNLDLMHGLPDQSIEDALSDLRIAIAQGPTHLSWYQLTVEPNTVFWNQPPVM
                     PEDDILWDIQEAGQALLAAEGYAQYEVSAYAQAGKAARHNLNYWTFGDFLGIGAGAHA
                     KLSHPDGNISRSWKTRLPKDYLDSSKRFSAGERPLSADELPFEFLMNVLRLTDGVASE
                     LFTQRTGLPLSQLAAARAEAEQRGLLHSDPTRLSATREGQLFLNDLLQHFLP"
     gene            9649..9972
                     /locus_tag="VFF22_03730"
     CDS             9649..9972
                     /locus_tag="VFF22_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3392 domain-containing protein"
                     /protein_id="HZX15964.1"
                     /translation="MDLLLDLIVTLSRWSRSHLYDISLAIMATLLVLFGPGINAWVQR
                     NTSSMNFIFRTLIFVLLCAVGYGLLIVFATPYLAKGLGFFNNFTLAPVLILVFFIIGI
                     LADRS"
     gene            complement(9981..11060)
                     /locus_tag="VFF22_03735"
     CDS             complement(9981..11060)
                     /locus_tag="VFF22_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HZX15965.1"
                     /translation="MSMMKAAVFIEPGRIELVDKPIPPVGPNDALVRITTTTICGTDV
                     HILKGEYPVAKGLTIGHEPVGIIEKLGSNVQGYQEGQRVIAGAICPSFSSYASQDGYS
                     SQDGGCSCHGYKPMGGWRFGNSIDGTQAEYVLVPDAQGNLAPVPDGLTDEQVLMCPDI
                     MSTGFAGAEAANIKIGDVVAVFAQGPIGLCATAGAKLRGASTIIVVDGVEQRLQIARK
                     MGADVTLNFNQVDVVEEILRLTGGRGVDAAIEALGLQSTFESALRVLKPGGTLSSLGV
                     YSTDLVIPLGAFHAGLGDHKIVTSLCPGGKERMRRLINVVASGRVDFGALVTHQYSLD
                     DIVAAYDLFAHQRDGVLKVAIKPFS"
     gene            complement(11237..12580)
                     /locus_tag="VFF22_03740"
     CDS             complement(11237..12580)
                     /locus_tag="VFF22_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HZX15966.1"
                     /translation="MLRTLSILLLATCALQAQAKVDSTQAARLGQDLTPLGGERAGNA
                     AGTIPAWDGGLAQPPANYQPGMHHPDPYSADAPLYQVNSQNLAQYQAQLPVGLKALLE
                     KNPDYFLRVFPTRRSAAAPQRIYDATRFNAQTAELISGGNGVDGAAAGIPFPLPKTGQ
                     EAIWNHIMRYRGDQISFVTNQAAVLSNGAYNLLKLERDIYFLYGREGVSPQDLDNTLF
                     YYKYKVVAPAKLAGSALVVQETLDQVLAIRKAWRFNRGERRVRRLPMLAYDTLQPDSN
                     GMATADVVDSYNGAPDRYEWTLVGKQEMLVPYNSYAVHQKGIPYDNILRSNTVNPELL
                     RYELHRVWVVDAELRKGFSHPYAKRRFYIDEDSWQILAADLYDKSGELSGVQESHPIS
                     YYDVPMFGSTLETIYDFKGKRYFVDGLDNNEPMYNFNAKLTPRDFTPQALRREGN"
     gene            complement(12764..13483)
                     /gene="trmB"
                     /locus_tag="VFF22_03745"
     CDS             complement(12764..13483)
                     /gene="trmB"
                     /locus_tag="VFF22_03745"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HZX15967.1"
                     /translation="MTELQQQPEPAEDTSKHRAIKSFVMRAGRMTEGQQRGLDQGWPK
                     FGLQLEDGLRDFDQVFGRSAPRTFEIGFGMGHSTLEMAAASPEQDFIGVEVHRPGVGA
                     LLNGLMTQNLSNMRVYSCDAMDVLRNCVADASLDRLLLFFPDPWHKAKHNKRRIVQPA
                     FAELVRQKLKVGGVLHMATDWQPYAEYMLGVMNVAPGYQNQADDGQYVPRPTERPTTK
                     FERRGERLGHGVWDLKFQRID"
     gene            complement(13504..14298)
                     /locus_tag="VFF22_03750"
     CDS             complement(13504..14298)
                     /locus_tag="VFF22_03750"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HZX15968.1"
                     /translation="MSHAPSDKPFTLAGRTYQSRLLVGTGKYKDLEETRLAIEASGAE
                     IVTVAVRRTNIGQNPGEPNLLDVISPDKYTILPNTAGCYDAVEAVRTCRLARELLDGH
                     KLVKLEVLADQKTLFPNVIETLKAAEVLVKDGFDVMVYTSDDPIIARELAAMGCIAVM
                     PLAGLIGTGLGICNPYNLRIILEEATVPVLVDAGVGTASDATIAMELGCEAVLMNSAI
                     AHAQNPILMGEAMKHAIIAGRMAYLAGRMPKKLYASASSPLDGLIK"
     gene            complement(14346..14546)
                     /gene="thiS"
                     /locus_tag="VFF22_03755"
     CDS             complement(14346..14546)
                     /gene="thiS"
                     /locus_tag="VFF22_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HZX15969.1"
                     /translation="MHIQLNGEAFELPDGTTVAGLIDRLDLAGRRVAVELNLDIVPRS
                     QHATTALRDGDQVEVVHAIGGG"
     gene            complement(14649..15035)
                     /locus_tag="VFF22_03760"
     CDS             complement(14649..15035)
                     /locus_tag="VFF22_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003084513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HZX15970.1"
                     /translation="MARLWLLLSACAGFTGVALGAFAAHGLKSQLTPAYLAVFQTGTH
                     YQLIHALALFGVGLLAMLRPAPLVNCAGALFALGIVLFSGSLYALTLSGISALGMVTP
                     FGGLAFLAGWLCLGMVAWKLGARGIA"
     gene            15103..15840
                     /gene="mtgA"
                     /locus_tag="VFF22_03765"
     CDS             15103..15840
                     /gene="mtgA"
                     /locus_tag="VFF22_03765"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HZX15971.1"
                     /translation="MFRSITRRLLKLLLWLILGSVLLVLLFRWVPPPGTALMVERKIE
                     SWLDNRPIDLQRSWRPWSELPDDLKMAVIAGEDQKFAEHWGFDVAAIQAALEHNQQGG
                     SLRGASTLSQQVAKNLFLWSGRSWLRKGIEVWFTGLIELLWPKQRILEVYLNSVEWGD
                     GVFGAEAAARHHFKVGAPYLSRQQSSLLAAVLPNPREWSASRPSAHISRRANWIRQQM
                     WQLGGSHYLDRLQPSRPEWWPSWLKDV"
     gene            complement(15842..16453)
                     /locus_tag="VFF22_03770"
     CDS             complement(15842..16453)
                     /locus_tag="VFF22_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15972.1"
                     /translation="MKLPPIRPQHWLIFAVVAALFFAWGAWLMRPQPAAQPLPWLAEW
                     QAKVLTPLADDRLSLVHLKDALEGELWLQPNLDGARLLYRGELQDADGNWSLEAEVGL
                     DKAERESLLAAAGLKPTDKEQPLGSQLLVPLANRPIIALTLLPPAPLDVARLAASIGQ
                     PRLTLELAEGQAWVYPELGMTAHLQDEELYFLYAVPRHLLKQR"
     gene            complement(16514..17368)
                     /gene="rpoH"
                     /locus_tag="VFF22_03775"
     CDS             complement(16514..17368)
                     /gene="rpoH"
                     /locus_tag="VFF22_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HZX15973.1"
                     /translation="MSTSLQPVHALVPGANLEAYVHAVNGIPLLTPEQERELAESLFY
                     KQDLEAARQMVLAHLRFVVHIARSYSGYGLAQADLIQEGNVGLMKAVKRFNPEMGVRL
                     VSFAVHWIRAEIHEFILKNWRIVKVATTKAQRKLFFNLRSQKKRLAWLNNDEVHAVAA
                     SLGVEPREVREMESRLTGHDMAFDPAAEADDESAFQSPAHYLEDHRYDPARQLEDSDW
                     SDSSTANLHEALEGLDERSRDILYQRWLAEEKATLHDLAAKYNVSAERIRQLEKNAMN
                     KLKGSIAA"
     gene            complement(17481..18500)
                     /gene="ftsX"
                     /locus_tag="VFF22_03780"
     CDS             complement(17481..18500)
                     /gene="ftsX"
                     /locus_tag="VFF22_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HZX15974.1"
                     /translation="MSATRMPPPQPAQRVGAAPKKSESPTPGDDGPSFTHQLHAWLEN
                     HRASLVDSLRRLGKQPIGSFFTCLVMAVALSLPMGLALLLDNVERLGVSWQRAAQISL
                     FLQIDATDAQGQALREQIAEMADVEEAEWISREQALDEFQQQSGLGEALKELPDNPLP
                     GVIVVTPQQVDKVALEALRLRLAELPRVQQAQLDLLWVERLSAMLKLGERFVFGLTLL
                     LVMALLLVIGNTIRLHIENRRTEIEVIKLVGGTDSYVRRPFLYMGTLYGLGAGLLAWL
                     LLAYGLGWLNDAVVRLAGLYGSDFALAGVPIADGLSLLLGAVLLGYIGAWLAVARHLS
                     ELAPR"
     gene            complement(18497..19165)
                     /gene="ftsE"
                     /locus_tag="VFF22_03785"
     CDS             complement(18497..19165)
                     /gene="ftsE"
                     /locus_tag="VFF22_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HZX15975.1"
                     /translation="MIKFEQVGKRYPNGHVGLHELSFHVRRGEFLFVTGHSGAGKSTL
                     LRLLLAMERPSSGKLLLAGQDLSHITNAQIPFLRRQIGVVFQNHQLLFDRSVFDNVAL
                     PLQILGLSKDEIGTRVRTALERVSLADKAEQSPGDLSTGQQQRVGIARAVVHRPALLL
                     ADEPTGNLDPRLAAEIMGVFEDINRLGTTVLIASHDLALIARMRHRMLTLQRGRLIGD
                     GEAA"
     gene            complement(19167..20588)
                     /gene="ftsY"
                     /locus_tag="VFF22_03790"
     CDS             complement(19167..20588)
                     /gene="ftsY"
                     /locus_tag="VFF22_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZX15976.1"
                     /translation="MFGSNDDKNTPAPSTPASAEKPAEKKDLFGWLRKKPQAPSSPEP
                     QLTPVIEQPAAPATEVATPASEPVAPVSEVAAPVESPAIAVEPQPVPVAPVEQAAAAI
                     PAPAEGSEANPQPRPSRFRINAGSEVLHAHIEVPAVVEPVVEPPVAAPAVVQPAAAPV
                     KSAEEQRAGFLERLKQGLSKTSASLGEGMASLFLGKKAIDDDLLEDLETRLLTADVGV
                     EATTAIIGNLTKRVARKELADSGALYKALQDELVALLKPVEQPLQIDSSKQPYVILVV
                     GVNGAGKTTTIGKLAKKLQQDGKKVMLAAGDTFRAAAVEQLQVWGERNQIAVIAQHTG
                     ADSASVIFDAVQAAKSRGMDVLIADTAGRLHTKDNLMEELKKVRRVIGKLDDTAPHEV
                     LLVLDAGTGQNAINQAKQFNQTVNLTGLALTKLDGTAKGGVIFALAKQFALPIRYIGV
                     GEGIDDLRTFEAEAFVQALFAEK"
     gene            20794..22149
                     /locus_tag="VFF22_03795"
     CDS             20794..22149
                     /locus_tag="VFF22_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZX15977.1"
                     /translation="MHMIAQRTAALLLGVLCTPLLALAAETQPTHEFSLDNGLKVIVR
                     EDHRAPVVVSQIWYKVGSSYETPGQTGLSHALEHMMFKGSRKLGPGEASRILRELGAE
                     ENAFTSDDYTAYYQVLARDRLAVALELEADRLASLKLPADEFAKEIEVIKEERRLRTD
                     DKPSSLAYERFKAMAYPASGYHTPTIGWMADLERMTVEELRDWYEAWYAPNNATLVVV
                     GDVSVAEVKTLAERYFGPILKRTVPTAKAPRELAAPGERRITLHLKTQLPSLMMGFNV
                     PGLATAEQPRQVHALRLAAALLDGGYSARLPTRLERGEELVSGASAWYDGFSRGDSLF
                     ILSATPNVQTGKTLDQVEAGLWRELEDLKNTPPSAEELARVRAQVIAGLVYERDSITS
                     QATAIGQLETVGLSWQLIDKELAELEAVTPADIQQAASTFFTRDRLSVAHVLPEEKRN
                     E"
     gene            22142..23623
                     /locus_tag="VFF22_03800"
     CDS             22142..23623
                     /locus_tag="VFF22_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003084496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZX15978.1"
                     /translation="MSERNGLRYGLLGLALLIVLALLALVINRPDSSPPPAPAAESTS
                     IESLAALGDQPPSRRDLNIQTWQTAEGAKVLFVEARELPMFDLQLTFAAGSSHDDGVP
                     GLAMLTNAMLNEGVPGKDVGAIAAGFENLGANFGNGAFRDMAIASLRSLSAADKREPA
                     MQLFNQVLGQPTFPEDALARIKNQLLAGFEYQKQNPGKLASLALFERLYGTHPYAHPS
                     DGNAESIPSISREQLQAFHAQAYTANNAIIALVGDLSRSDAEALASQVSAALPKGPAL
                     PRIAQPEEPKPGLQHIEFPSNQTHLMIAQLGIDRRDPDYAALYLGNQVFGGGGFGTRL
                     MTEVREKRGLTYGVYSGFSAMQARGPFMINLQTRAELSDGTLQLVKSMLADFIAYGPT
                     TDELNNAKRELAGSFPLSTASNAAIVGQLGSMGFYDLPLSYLEDFMRDVEALSVEQVK
                     AAMAKHLNPDALVIVTAGPSVTQKELPPPTDRPAEQPSGVPEH"
     gene            23623..24231
                     /gene="rsmD"
                     /locus_tag="VFF22_03805"
     CDS             23623..24231
                     /gene="rsmD"
                     /locus_tag="VFF22_03805"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HZX15979.1"
                     /translation="MSKRPKPPKANPAHSGDGQLRIIGGQWRSRQFNFHMAAGLRPTP
                     NRVRETLFNWLAPYVEGAKVLDLFAGSGALFLEALSRGAGNALALDLNPAAIASLRSH
                     LQTLKCDNGQLQQTDALLFLQNPAVTPFDLVFLDPPFNQNLLLPACTLLEQNGWLAAD
                     AWVYTESENSPSSLGMPGNWRLHREQKAGQVYYALWQRTAAA"
     gene            24359..25348
                     /locus_tag="VFF22_03810"
     CDS             24359..25348
                     /locus_tag="VFF22_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HZX15980.1"
                     /translation="MTTPFTPAWWLPGPHLQTLWNPMCRKPAKLARTRERLWLDDGDF
                     LDLDWHGPHQADAPLVLVLHGLTGSSDSLYVLGLQQELAAQGWASVALNWRGCSGEPN
                     LLARGYHSGASEDLAETVRHLRAQRPMAPIYAVGYSLGGNVLLKYLGESGADSQLQGA
                     AAVSVPFRLDQCADRIGMGFSRVYQSHFMREMVAYVKDKQRLFAHQGMADRLSTLEKL
                     GSLDNMRTFWDFDGRITAPLHGYADAEDYYRRASSRYFLGQIETPTLIIQAADDPFVF
                     THSLPEASELSPSIEFELHAHGGHVGFVEGSLGKPGYYLERRIPQWLKQQFNP"
     gene            complement(25400..26059)
                     /locus_tag="VFF22_03815"
     CDS             complement(25400..26059)
                     /locus_tag="VFF22_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZX15981.1"
                     /translation="MSAPLKTRERIIQESLALFNAQGERSVTTNHIAAHLGISPGNLY
                     YHFRNKQAIIAELFTLYESQVDTFLRRPEGRALTVQDKTFYLEALLAAMWHYRFLHRD
                     LEHLLDSDAELAERYKLFSQRCLQRAQNIYQGFVEAGILLMNAPQIEALTLNSWIIMT
                     SWVRFLCTTRGNPGDLSQEMLRRGIYQVLALEGGYITPSAREAVEALYAKLHVPLEQV
                     I"
     gene            26296..27738
                     /locus_tag="VFF22_03820"
     CDS             26296..27738
                     /locus_tag="VFF22_03820"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HZX15982.1"
                     /translation="MVADVAYLEQSLQRSQQQLSQLESSFTRQRQAFKANPMPSAEQR
                     IQWLNVLRDLLSNERDALISAISQDFSNRSADETLLAELMPSLHGIHYATKRIKKWMK
                     PSRRSVGMQFMPAAAKVIYQPLGVVGVIVPWNYPLFLAIGPLVGALSAGNRVMIKMSE
                     STPATSQLIKDLLAKVFPEDLVTVVLGEAEVGMAFSKLPFDHLLFTGATSIGKHVMRA
                     AAENLTPVTLELGGKSPAIVSASVPLSDAAERIAFGKTMNAGQTCVAPDYVLVPKDRV
                     EGFVEAYRKAVQSFYPTLANNPDYTAIINERQQQRLNGYLQDAESKGATIIPLYPEAQ
                     GRRMPQAVLLNVTDEMKVMQDEIFGPLLPIIPYDKLEDAFAFINERDRPLALYYFGYD
                     RSEQQRVLHETHSGGVCLNDTLLHVAQDDMPFGGVGPSGMGHYHGHEGFLTFSKAKGV
                     FIKQRFNAAKLIYPPYGKALQKLVYKLFVR"
     gene            27771..28316
                     /locus_tag="VFF22_03825"
     CDS             27771..28316
                     /locus_tag="VFF22_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008048318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal
                     protein"
                     /protein_id="HZX15983.1"
                     /translation="MHNTTLEAPALTRRNLLKIGLMGSAFLATAGLAASLSGCSASTP
                     KAGFAVIRESDLAFLNALIPVMLAGAVTTEKMPAAVSATLENLDYSLNHLSPEMLKLT
                     VQLFDVLAMPITRGPLTGVWGGWENASPADVQGFLDRWQNSPIGLLKMGHASLLQLVM
                     MSWYSRAESWAHCGYPGPPTV"
     gene            28464..30059
                     /locus_tag="VFF22_03830"
     CDS             28464..30059
                     /locus_tag="VFF22_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZX15984.1"
                     /translation="MPVPDMFKQGLANGWKTYNGSRLDSDLTLEADVAIVGSGAGGGT
                     TAEILSAAGFKVLLIEEGPLKTSDDFKMLEDKAYASLYQEGIGRMSKDGAITIMQGRA
                     VGGTTLINWTSSFRTPAPTLEHWAKEHGVKGHSPEEMAPWFEQMEQRLGVAPWAVPPN
                     ANNAVISSGCEKLGYEWKVIPRNVRGCWNLGYCGMGCPTNAKQSMLVTTIPATLDKGG
                     ELLYLARADKLLIEGDQVTGIECLGMDERCVAPNGRKITVKAKHYVLSGGGINTPGIL
                     LRSNAPNPHQRTGKRTFLHLVNFSAALFDQVINPFYGAPQSIYSDHFQWDDGTTGRLS
                     YKLEVPPLQPAMTSTLLGRFGIDNAMRMEQLPNTNVMLALLRDGFHPDSAEGRVELRG
                     DGSPVLDYQMTDYTWDGVRRAFHSMAEIQFAAGAKAVLPLHADAGYVKTLGEAKQLID
                     TLPLEIYRTRLGCAHVMGGCAMGEDPRQAVTDSLGRHHQLSNLSIHDGSLFPTSIGAN
                     PQLSIYGLTAKLATKLAERLKHA"
     gene            30182..30661
                     /gene="coaD"
                     /locus_tag="VFF22_03835"
     CDS             30182..30661
                     /gene="coaD"
                     /locus_tag="VFF22_03835"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003176711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HZX15985.1"
                     /translation="MNRVLYPGTFDPITKGHGDLVERAARLFDSVIIAVAASPKKNPL
                     FSLEQRVELAREVTQHLPNVEVVGFSTLLAHFAKEQGANVFLRGLRAVSDFEYEFQLA
                     NMNRQLAPDVESLFLTPSEKYSFISSTLVREIAALGGDISNFVHPAVATALTERFKR"
     gene            30770..31021
                     /locus_tag="VFF22_03840"
     CDS             30770..31021
                     /locus_tag="VFF22_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HZX15986.1"
                     /translation="MSLIITDDCINCDVCEPECPNAAISQGEEIYEIDPNLCTECVGH
                     YDEPQCQQVCPVDCIPLDENNVESKDELMQKYLIITGKA"
     gene            31018..32730
                     /gene="ggt"
                     /locus_tag="VFF22_03845"
     CDS             31018..32730
                     /gene="ggt"
                     /locus_tag="VFF22_03845"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZX15987.1"
                     /translation="MKTGRLIRGLLPWRTLLGALALLCALELHAAAQPERIAIASAHP
                     AATVAGQETLALGGNAFDAAVAISAALAVVEPYGSGLGGGGFFLLREAGESPTYRFLD
                     ARERAPQAAHADLYRVEGEINKDLSLNGALAAAIPGLPAALVELAERFGRLPLRDSLA
                     PAIRLARSGVSIDRVYRERAGWRLEAMRQDAETARIFLDKGEIPEEFGLLRQPELAST
                     LEQLGTKGLQGFYGGAVAEQLVKAVRDAGGIWTLKDLTDYRIVERQPLRVSLDQGREL
                     ISAPPPSAGGIALGQSLLMLQQLPWQQADRVQRTHLVVETLRRAYRDRGLLGDPDFVR
                     NPAQQLLAPDYLKQLASSIDPQRATPSDSLPPSKTWQEGDHTTHFSVLDTEGNAVAAT
                     LSINLPFGAAFTAPGTGVLLNNEMDDFAADVQGSNAYGLSGSQANSITAGKRPLSSMS
                     PSFIESTEDFASFGTPGGSRIPSMVLLAMLQYLDQQPIERWPSVSRYHHQFRPDLIEH
                     EPDTFSPAEITALEALGHQLKSTGRQYGNQQVLYWDKQGGKVAAAEDPRGIGTAVVTP
                     AVPQ"
     gene            complement(32812..33150)
                     /locus_tag="VFF22_03850"
     CDS             complement(32812..33150)
                     /locus_tag="VFF22_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HZX15988.1"
                     /translation="MKLFRSTAAALALTTGLLALPAYADVAQQNSSGDPLYTANAPKA
                     FSMIGDLLVARPLLIGATAIGAVAFVVSLPFTAAGGNIGEAGKALVVEPGEAAFVRCL
                     GCTTSGYKKN"
     gene            complement(33317..34129)
                     /gene="mutM"
                     /locus_tag="VFF22_03855"
     CDS             complement(33317..34129)
                     /gene="mutM"
                     /locus_tag="VFF22_03855"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HZX15989.1"
                     /translation="MPELPEVETTRRGIAPFLEGQRVSRVIVRERRLRWPIPEDLDIQ
                     LSGQRIECVERRAKYLLIKAETGSLISHLGMSGSLRLVEAGLPAAKHEHVDIELESGL
                     ALRYTDPRRFGALLWSSDPLNHVLLSKLGPEPLTELFDGERLFQLSRGRAMAIKPFIM
                     DNAVVVGVGNIYASEALFAAGIDPRREAGSVSRARYLTLADEIKRILVHAIERGGTTL
                     RDFVGGDGQPGYFQQELFVYGRGGEFCKHCGSTLREVKLGQRASVYCPRCQR"
     gene            complement(34204..35016)
                     /locus_tag="VFF22_03860"
     CDS             complement(34204..35016)
                     /locus_tag="VFF22_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HZX15990.1"
                     /translation="MPPQPQIMVDLQMEQVMPSPDLRAIAKLISQDPGLSGALLKLVN
                     SPFFGLANRIASIQQAVNLLGCNTVINLINAQSIRGELTDEAIVTLNRFWDTAQDVAM
                     TCLTLAKRIGYHSPDEAYTLGLFHNCGIPLMIKRFPNYMNVLEEAYATATGERRVVDT
                     ENRLLNTNHAVVGYFTAKSWNLPLHLCEAIASHHNALAIFTEDASRDAQLKTLLAILK
                     MAEHICASHRVLGNQPDDHEWQSIEQLVLEYVGLSDYDFENLRESIRDLGAS"
     gene            35202..36398
                     /locus_tag="VFF22_03865"
     CDS             35202..36398
                     /locus_tag="VFF22_03865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019097921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HZX15991.1"
                     /translation="MSLPSLRLKANADRRLRAGHLWVYSNEIDVAATPLSGFQAGDQA
                     ILEATGGKPLGVVAMSPNNLICARLLSRDVKHVLDKSLLVHRINVALSLRERLFDKPC
                     YRLVYGDSDLLPGLVVDRFFDILVVQLASATMERHKDDVLAALIQVCKPSGILLKNDS
                     AARDAEGLARYVETPFGVVPEWVALEENGVKFEAPVIEGQKTGWFYDHRMNRARLAPY
                     VKGKRVLDLFSYIGGWGVQAAAFGASDVMCVDASGFALDGVERNAALNGLTDQVACVE
                     GDVFEALKQLKASEERFDVIVADPPAFIKRKKDQKNGEGAYRRLNEQAMRLLNKDGIL
                     ISASCSMHLEEDNLQNILLTSARHLDRNIQLLERGAQGPDHPVHPAINETRYIKSLTV
                     RLLPNS"
     gene            36674..37789
                     /locus_tag="VFF22_03870"
     CDS             36674..37789
                     /locus_tag="VFF22_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX15992.1"
                     /translation="MADLAGDKPFLQFTNVKKTYDHKNLVVKDFNLDVAKGEFITLLG
                     PSGSGKTTCLMMLAGFEDVTSGEILINGRSVTSIAPYQRNIGMVFQQYALFPHMTLRE
                     NLAYPLKIRKQPKEVIRAKVDEYLSLVELLDFGNRFPGQLSGGQRQRVALARALIFAP
                     DIVLMDEPLGALDKKLREQMQFEIKRLHEQLGFTVIYVTHDQTEALTMSDRIAVFNNG
                     IVQQCAPPHVLYERPANLFVADFIGESNKLKGVIESVGSETVEVRLDDGAMVSSLKAN
                     CTEVGLPTTVSIRPEKLTFTDRLGAAGNQVMARFVTRHYVGEYIRYHFETADGTELVV
                     KNMNDSSAPQLSAKEEVALAWLPQDSQALDRPIATPT"
     gene            37827..38900
                     /locus_tag="VFF22_03875"
     CDS             37827..38900
                     /locus_tag="VFF22_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX15993.1"
                     /translation="MARTLTTRFSTFALAAALGTASFAASAQETLTVVSWGGAYGAAQ
                     QKHVIDPFMKDTGNKVLFEDYTGGVAEIKAQVESGNIQWDVVDFEVIDLERACSEGLL
                     ESIPRDILPAGIDGVPAEKDFIPEALASECAVGNIVWSVVFAYNEKTIGGTKAASITD
                     FFDTSKIAGKRALRKRPQVNMEWALLADGVPPGEVYDVLSTPEGQARAFAKLDTIKKD
                     IVWFDSWSQAPQLLNDGGAVLVQSANGRFYDAIRQENKPFVIVWDGHVYDLDAWAIVK
                     GSPKKDLALKFIAYATGSTPLAGMPDVAYGPTRKSSMPLADQSAAPHLPTAHLSKGIQ
                     AGSDFWADYGESLGEKFNEWLLK"
     gene            38955..40187
                     /locus_tag="VFF22_03880"
     CDS             38955..40187
                     /locus_tag="VFF22_03880"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX15994.1"
                     /translation="MSSLTSTEASQASSARNKKRLTAFLFVVPLLVFILLTFVAPIAS
                     MLWRSVHHPTVAALIPLTLSELQRWDGKAIPDEKTLSVFAQELHSLAKERQSGKLAEE
                     FNRASAGMSSVVKSTARKLSRLQDAQLQQEGADILLKSHRNWSKPEIWYAIQRAGKPY
                     TYDYYLTALDLELHQEQGIQVREDTQIYLQLYSKTLTMALVITIFCALLGYPLAYYLA
                     GLPENRANLLLVLVLLPFWTSLLVRTTAWIALLQTNGVINSFLLGIGVIGQPIEMLYT
                     SFATVVAMTHILLPFMILPLYSVMRGIDPSYLRAALSLGARPIPAFLRIYFPMTLPGL
                     SAGALLVFIISVGYYITPALVGGTDGQMISNIIAFHMQRSNNWELAAALGSLLLGLIL
                     LLYWLYDRFVGASNIKLG"
     gene            40198..41052
                     /locus_tag="VFF22_03885"
     CDS             40198..41052
                     /locus_tag="VFF22_03885"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX15995.1"
                     /translation="MKIPAYYGFWHHLGAWLLKTSSWLVLLFLILPILVIIPLSFNVE
                     PFFSFTEGMLTLQPEAYSLRWYSAIFSDDKWLLAIKNSFLIGIFATLIATVLGTCAAV
                     GLARDDMPMRRLITALLLSPMIVPLIITAAGMFFFYSDLGLAGNYLGVIIAHAALGTP
                     FVIITVTATLTGFDYSLARAALNLGATPLRVFFDIIMPLIRPGVISGALFAFITSFDE
                     VVVILFMAGPQQRTIPRQMFSGLREQINPSILAIATLLILVSIALLVTIELLRRRAER
                     MRGIELPT"
     gene            complement(41083..41928)
                     /locus_tag="VFF22_03890"
     CDS             complement(41083..41928)
                     /locus_tag="VFF22_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX15996.1"
                     /translation="MLDLKPGGLAAASLFISISALAAPLPDTPGAPFPNVSSFERSGP
                     YATSNQSEGPSCRIYRPSNLGQGGVRHPVILWGNGTGTGPSTYAGLLSHWASHGFVVA
                     AAETSNAGTGQDMLACLDYLVQENSNAYGTYAGKLNTGRVATSGHSQGGGGSIMAGQD
                     ERVRTTAPIQPYTIGLGHDTASQRRQQGPMFLMSGGGDTIAFPYLNAQPVYLRTNVPV
                     FWGERRFVSHFEPVGDGGAYRGPTTAWFRFQLMDDQSARGTFFGARCSLCSSLQWSVN
                     RRGIQ"
     gene            42401..44248
                     /gene="ilvD"
                     /locus_tag="VFF22_03895"
     CDS             42401..44248
                     /gene="ilvD"
                     /locus_tag="VFF22_03895"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZX15997.1"
                     /translation="MPDYRSKTSTHGRNMAGARALWRATGMKDEDFKKPIIAIANSFT
                     QFVPGHVHLKDMGQLVAREIEKAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSREIIAD
                     SVEYMVNAHCADAIVCISNCDKITPGMLLASLRLNIPVIFVSGGPMEAGKTKLASHGL
                     DLVDAMVIAADDSASDEKVAEYERSACPTCGSCSGMFTANSMNCLMEALGLALPGNGS
                     TLATHSDREQLFLQAGRTAVELCKRYYGEGDSSVLPRNIANFKAFENAMTLDIAMGGS
                     TNTILHLLAAAQEGEVDFDLRDIDRLSRKVPQLCKVAPNIQKYHMEDVHRAGGIFSIL
                     GSLARGGLLHTDLPTVHSKSMAEAIAKWDITQTDDEAVHTFFRAGPAGIPTQTAFSQS
                     TRWDTVDDDRENGCIRSVEHAYSQEGGLAVLYGNIAVDGCVVKTAGVDESIHVFEGNA
                     KIFESQDSAVRGILADEVQAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLGKACAL
                     LTDGRFSGGTSGLSIGHASPEAAAGGAIGLVREGDKILINIHERSINLLISDEELAAR
                     RVEQDKKGWKPVEVRPRKVTTALKAYALLATSADKGAVRDKALLDKLVP"
     gene            44356..48384
                     /locus_tag="VFF22_03900"
     CDS             44356..48384
                     /locus_tag="VFF22_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX15998.1"
                     /translation="MLVARRVQKTAEKHDEESRLEELPLSDFRHTPAWVLLGEPGAGK
                     SEALRQEALEVGGEYVTVSEFVETTPPLAEWYGKTLFIDALDEARASGSVSLILHIRQ
                     QLRQLGKPAFRLSCRAADWHGSTDKTELQSISPDNQLTVLQLCPLSDDDIQQILQNQF
                     NIAEPKTFIEQAEQHGIAQLLSNPQMLGLMARAVSGGSWPESRSEVYRLACEKLAQES
                     NALHRQISKAKAVDGETLLKAAGQLFAIQLLSGKAGIATDLNASNTGNPELDQFKPES
                     SAAARKALQSALFTQAPGQAERLAPCHRSIAEYLAAKWLANKLDRESLPQGRLLNLLL
                     GFDGGVVADLRGLLAWLAHHSTSIRSRLIKVDPLGVVLYGDVRPFSPENKRHLLHALR
                     QQAERFPGFRWSLWQDMTGFRALADEALLEDFLRTLQAPERDKASQSHMDCVLDILEY
                     SHTYACMAAPLLAVIKDASLWPRLRYSALRIWFKLATPIESKVLLSQICSDQVEDEDD
                     ELLSRLLDRLYPEFLIPDELLQCIHSPKNTHLIGGYRMFLEHGLSKLAPANQLPALLD
                     GLCAKRDFFDEDKTERMVSRSVDRLLVRTLNELGDFATDEQLLNWLGVGTNKHGYFNR
                     DHDSRAQIQRWLEQRPEKYKAVLAQCVEANARGSKHASYLLLHDAAPPADIGLWHLHL
                     AGIPEEATARIHAQYATHALMHQRGAEGLSLEALEAWARDFPERADWLNEGLVCDLER
                     ESWRLEDAARTRPYRERQAEERRQRTQEITPELPAIAAGTAAAELLEELALVWLNGLH
                     DIYGDTISERFASYSDLGDVLMQAAESGFRNCLQRPALPSVAEVIDMDQAGKRDYISL
                     PYLLGLQLSWADAPEQVTALPDDQVELLLAIYLCTPLFGLDSLSAWCAQTAQQRPELF
                     ARVLTRQATNAFAAGNTSAGMAYSLARDASYTKVAQLTSPELLRQFPESATEQQLHDL
                     RQLLRAALVTAPECLAALALNKLKSPQLNTEQRLLWLALQLLTGNPEDESEFWQRLGE
                     AEEANVAMQLAVEVLRQGIQLQPCQPERIVGRLIETLIPHAELEHRKGGGFVTTAMDL
                     GDELRSMIDWLGAQPTVEANNELQHLLACPALATQVYRIQSTLEQQQARRREAEFKFL
                     DLKAVADVLANQAPANVADLTYLTLSHLDDIAHELRNANDDGYRMFWNITKAQPTGRR
                     EENLCRDVLLTRLRSRLTAHGISIAPEYDHAGDKRADLYLDYRNQLALPIEVKRDDHA
                     KLWTALCDQLITQYVDAPKSEGHGIYLVLWFGESKNLKSLPNRQPKPTTPAELKTKLE
                     AQLTSAEQQKVFVRVLDVSWPT"
     gene            complement(48392..49039)
                     /locus_tag="VFF22_03905"
     CDS             complement(48392..49039)
                     /locus_tag="VFF22_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020305627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HZX15999.1"
                     /translation="MHDADANHAEPDRIAAIFIRHPLWEDGLALLLGTAMVALGITLY
                     GQAGLLTGGTVGLAFLMKYLLGWPFGLVFFLINLPFYALAIWRMGWPFTLRTFCAVGM
                     VSLLAELTPQWIGFTHLNVVYAALFGGFAMGLGLLMLFRHRASLGGVNILALYLQERF
                     GLRAGKVQMGIDVMIVMGAVMVVPLDKVALSILGALALNMVLAINHRAGRYMGVS"
     gene            complement(49207..49731)
                     /locus_tag="VFF22_03910"
     CDS             complement(49207..49731)
                     /locus_tag="VFF22_03910"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HZX16000.1"
                     /translation="MLAAMNKTLPLSLIAALAENRVIGRDNQLPWHLPADLKHFKALT
                     LGKPIIMGRKTWDSLGRPLPGRLNLVVSRQPGLQLEGAEVFASLEAALERADAWAREE
                     DAEELMLIGGAQLYELGLAQAERLYLTRVGISPQGDAHFPAFVEADWHLSSAIEHPAG
                     LETPGYVFEVWDRK"
     gene            49943..51034
                     /locus_tag="VFF22_03915"
     CDS             49943..51034
                     /locus_tag="VFF22_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HZX16001.1"
                     /translation="MTSPAQADSVQAAIDWARQQPQTHHGKPGGDAKPLIIAHRGASG
                     YVPEHTLAAYALAVLQGADYIEPDLVTSKDGQLVARHDNELGLTTDVAQRPEFANRQR
                     TQTIDGVTLNGWFSEDFTLAELKTLRAIERIPQIRPGNARLDGNFSIPTLQEIIDLVK
                     SLQLSQQRVIGLYPETKHPTHFQQLGLALEKPLVRLLKKNGYQNAKAPVYIQSFEVEN
                     LKTLSRLTSIRLVQLFGAGQPFDQQVLGTGLTYAQMASPAGLKAISRYAAGVGPEKSY
                     IIPRDANGNLDAANATAFVANAHAHGLKVHPYTFRAENTFLPANLRIGSDPQARGDSA
                     AEIQLFLDAGIDGLFIDQPDIAVRLRAKP"
     gene            51095..52501
                     /locus_tag="VFF22_03920"
     CDS             51095..52501
                     /locus_tag="VFF22_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HZX16002.1"
                     /translation="MSRRVSPLDKSPPSRLHRLWLSETIRLREEHAGPLEDSEANRRA
                     QAQGGALDERIQTRAWLLGERDGQVQALQHWLQGARLAGLFLVLLAILSGSGLAVAAL
                     GDGLRPVNVFWALGSLLGLNLLLLLGWVLGLLLSRDSAGALGHLWLWLSAKLARDAQA
                     AQLAPALLLMLQRQRLSRWGLGVLVQGFWLLTLLTALLTLLALLTTRRYGFVWESTLL
                     GADAFVAITNALSVLPRALGFSAPTVAMIHASTSDVYNTELVRQSWAAWLIGAVLIYG
                     VLPRLGLLLLCLWRWRRGCAALSLDLSLPGYSLLRERLQPSSERLGVCDAAPAQLHQP
                     QAGSLAEGSQGALLVAVELDPQRSWPPALPKSIGDAGVIDSREQRNQLLDQLNRFPPA
                     RLLIACDPRRSPDRGSLALLGELACTASATRIWLLPPPPGEALDSARLGDWHRALEQL
                     QLSYSDSAPFSWLEHGHD"
     gene            52583..53950
                     /locus_tag="VFF22_03925"
     CDS             52583..53950
                     /locus_tag="VFF22_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HZX16003.1"
                     /translation="MSHPLKLAVVGHTNVGKTSLLRTLTRDVGFGEVSHRPSTTRHVE
                     GARLSVDGQALLELYDTPGLEDAIALLDYLERLDRPGERLDGPARLARFLDGSEARQR
                     FEQEAKVLRQLQASDAGLYVIDAREPVLAKYRDELAVLSMCGRPLLPVLNFVSSSQHR
                     EQDWRDALARLGLHALVCFDSVAPPLDGERRLYDSLALLQEKARPQLQRLIDDHLTQR
                     QLRKSSAARLIAELLLDCAACRRSVAAQAVLEQGAIRDLHQTIRQREQRCVEALLRLY
                     AFRQEDAKAGDLPLLDGRWGDDLFNPETLKQLGINIGGGMAAGAATGVGVDLLVGGIT
                     LGAAALIGAVLGGSAQTLRGYGARLKGKLKGQRELTVDDAVLRLLALRQQLLLQALEA
                     RGHAAVEAISLSTPQDASWREGKLSDALHKARAHPEWSSLNPGAQLEDGERQEQVQQL
                     AAELL"
     gene            complement(53986..54780)
                     /locus_tag="VFF22_03930"
     CDS             complement(53986..54780)
                     /locus_tag="VFF22_03930"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HZX16004.1"
                     /translation="MKQYLDLMRLVRETGTFKSDRTGTGTYSIFAHQMRFNLADGFPL
                     VTTKKCHLKSIIHELLWFLQGDTNIKYLKDNGVRIWDEWADENGDLGPVYGYQWRSWP
                     APNGESIDQISKLIEMIKKNPDSRRLIVSAWNPALVEQMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTLMVAQVCGLQPGDFVWTGGDCHLYANHIEQTDL
                     QLSREPLPLPVMKLNPEVKDLLAFKFEDFELVGYQAHPHISAPVAV"
     gene            complement(54777..55580)
                     /gene="lgt"
                     /locus_tag="VFF22_03935"
     CDS             complement(54777..55580)
                     /gene="lgt"
                     /locus_tag="VFF22_03935"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HZX16005.1"
                     /translation="MLPYPQIDPVALALGPLKIHWYGLMYLIGIGGAWWLASRRVNTF
                     APSWNKEKLSDLVFWVAMGVILGGRLGYVLFYDLAAYIAEPVRMLRIWEGGMSFHGGL
                     IGVMLATWWFGKRNNKSFFELMDFIAPLVPIGLGAGRIGNFINAELWGKATDVPWAMI
                     FPTDPQQLARHPSQLYQFALEGVALFAILWFYSRKPRPTMAVSGMFAVCYGIFRFIVE
                     FVRVPDAQLGYLAGGWLTMGQVLCVPMVLGGLGLIAYAYKRQAAQEAVQ"
     gene            complement(55590..56372)
                     /locus_tag="VFF22_03940"
     CDS             complement(55590..56372)
                     /locus_tag="VFF22_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011536220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZX16006.1"
                     /translation="MELLLYLVLGAAAGTLAGLFGVGGGIIIVPVLVLSFAAQGFDPA
                     ILTHLAVGTSLATIIFTSINSVIEHQRKGAVLWPVFAWMTLGILLGAGLGAMTATAIQ
                     GPLLQKIIGVFAIIIAVQMALELKPKASRTVPGKPALTLAGGVIGWASAIFGIGGGSL
                     TVPFLTWRSVAMQQAVATSSACGLPIAIASALSFIWLGWDKPALPQWSLGFVYLPALV
                     GIAIPSMYFARVGARLAHKLSPRLLKRLFALLLFSVGLSFLI"
     gene            56494..56862
                     /locus_tag="VFF22_03945"
     CDS             56494..56862
                     /locus_tag="VFF22_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16007.1"
                     /translation="MRPTLYLKLSCNQMHLMHLQSGRALHLQADPAFSNQRLLVADFA
                     AAQQLLQRGINELLPKRFLRLSRPPQLLIQPLERLEGGLSQVEERILLELGLGCGARK
                     VRLHLGNELDNAGVLAKLRS"
     gene            56866..57612
                     /locus_tag="VFF22_03950"
     CDS             56866..57612
                     /locus_tag="VFF22_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015637399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HZX16008.1"
                     /translation="MCLIVLAWRPGHAQPLLLAANRDEFYERPSQPLAEWLDLPGVIA
                     GRDLQAGGTWLGVGPNGRFAALTNIRDPGQAQGSRSRGELPVAFLAGSHSVEDFLHHL
                     HEQRRHYSGFNLLLGDNQQLWHYNSLTGRATPLAEGVHGLSNADLDTPWPKLQRAKQA
                     LHNGLHEPHPQMLLKLLADPTRAEDADLPHTGIGLTGERLLSSIFIASATYGTRASTA
                     LIVNADGSRVLAERTFGSNGNCLGEVVLQC"
     gene            complement(57628..58488)
                     /locus_tag="VFF22_03955"
     CDS             complement(57628..58488)
                     /locus_tag="VFF22_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16009.1"
                     /translation="MLNLADNFLQLRQLATLRLSAASALVCQGQSFWVLADDALVLQR
                     YSLSGDWQAELTLLPGELPDDAKQRKRLKPDFEALLLLPDDSLLALGSGSTKRRCRGC
                     LVEADNVRVIDLSPLYQALAEHFQDLNIEGGVVYRGQLLLAQRGNGHGRENALVVLDL
                     PQVLRDLQDGQLSAAALQRIVPLHLPELDGVPLSLTDLSMAPNGSLYFTATAEATDSS
                     YLDGACVGSVLGRLDAQLSILELNRLAPVVKIEGLAFQTDGRPLLVADADDPTIASPL
                     FTLDQFSESA"
     gene            complement(58502..60781)
                     /gene="ptsP"
                     /locus_tag="VFF22_03960"
     CDS             complement(58502..60781)
                     /gene="ptsP"
                     /locus_tag="VFF22_03960"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HZX16010.1"
                     /translation="MLNTLRKIVQEVNAAKDLKAALGIIVQRVREAMGSQVCSVYLLD
                     PESNRFVLMATEGLNKKAIGKVSMAPNEGLVGLVGTREEPLNLEHASEHPRYRYFAET
                     GEERYASFLGAPIIHHRKVMGVLVIQQKEQRQFDEGEEAFLVTMSAQLAGVIAHAEAT
                     GSIRGLGRQGKGIQEAKFVGVPGSPGAAVGTAVVVLPPADLEVVPDKTVDDIAAELTL
                     FNNALEGVRSDMRALSAKMATQLRPEERALFDVYLMMLEDAALGNEVVKVIRTGQWAQ
                     GALRQVIGEHINRFELMDDAYLRERASDVKDLGRRLLAYLQQARQQTLVYPDNCILVS
                     EELSPAMLGEVPEGKLVGLVSVQGSGNSHVAIFARAMGIPTVMGVVDLPYSKIDGIQL
                     IVDGYHGEVFTNPSEVLRKQYAEVVEEERQLAQGLDALRALPCETLDGHRMPLWVNTG
                     LLADVKRAQERGAEGVGLYRTEVPFMIKERFPSEKEQLAIYREQLAAFHPLPVTMRSL
                     DIGGDKALSYFPIKEDNPFLGWRGIRVTLDHPEIFLVQTRAMLKASEGLNNLRILLPM
                     ISGIQELEEALHLIHRAWGEVRDEGTDVPLPPVGVMIEIPAAVYQVRELARQVDFLSV
                     GSNDLTQYLLAVDRNNPRVADLYDFLHPAVLQALRIVVDGAHAEGKPVSICGEMAGDP
                     ACAVLLMALGFDGLSMNATNLPKVKWLLRQISLSKAQELLAQIMTIDSPQVIHSTLQL
                     ALRNLGLGRMINPASDIQA"
     gene            complement(60800..61279)
                     /locus_tag="VFF22_03965"
     CDS             complement(60800..61279)
                     /locus_tag="VFF22_03965"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HZX16011.1"
                     /translation="MIDSDGFRPNVGIILTNDVGQVLWARRINQDAWQFPQGGINDHE
                     SPEEALYRELNEEVGLEQQDVKILACTRGWLRYRLPQRLVRTHSQPLCIGQKQKWFLL
                     RLTGAEDRVRMDLTGKPEFDGWRWVSYWYPLGQVVTFKREVYRRALKELAPRLLARD"
BASE COUNT        12459 a        19790 c        18001 g        11036 t
ORIGIN      
        1 gaaacccagg cggcgggcgc aagtaatgtc cacgatagca gagaatattg caaaggtcgg
       61 agtgcgcatc cgtgaggcgg cgcaagcctc gcagcgagat tgtgcgactg ttggcctact
      121 cgcggtgagc aaaaccaaac ccgccgcagc gattcgtcag gcgtttgccg ccggcacccg
      181 cgatttcggc gagaactacc tgcaggaagc cctggaaaag caggtcgaat tgagcgacct
      241 gccgctgacc tggcacttta tcggtccgat ccaatcgaac aaaaccaagc ccatcgccga
      301 gcactttgcc tgggtgcact cggtggaccg cctgaaaatc gcccagcgac tgtctgatca
      361 gcgcccgacg catttaccag cgctgaacat ctgcctgcaa gtgaatgtca gcggtgaggc
      421 cagcaagtct ggctgcaacc cggatgagct gcctggcctg gcacaagcgg ttacacaact
      481 gcccaacctg cgcctgcgcg gattgatgac aatccccgag cccaccgatg accccagcga
      541 gcaacgcgcc gccttcgccc gcttgcgtga gctgcagcaa ggcctgaacc tggacctcga
      601 caccttgtcc atgggcatga gccatgacct ggaagcggcg attgccgaag gtgcgacctg
      661 ggtacgcatc ggcactgcac tgttcggcgc gcgcgactac agcagcaact gattgagttt
      721 gccttcagcc ttattaagga agccgttatg accactcccc gcatcgcctt tatcggcgca
      781 ggcaatatgg ccgccagcct gatcggcggc ctgcgcgccc agggcatcga cgccggcgcc
      841 attcgcgcca gcgaccgcgg tgccgaacag cgcagcaaga tcagcgccga gcacggcatc
      901 gaaacctttg ccagcaatgc cgaggccatt cagggcgcgg acgtgatcgt gctgtcgatc
      961 aagccgcagg tgatgaaaga ggtgtgcctg gatctcgccg cgcatatcag cgggcagcag
     1021 ctggtggtgt cgattgccgc cggcatcagc tgtgccagcc tggaaagctg gctcggccca
     1081 cgtgcaattg tgcgctgcat gccaaacacc ccggcgttgc tgcgtcaggg cgttagcggc
     1141 ctgtatggca atgcacacgt atcgcaggcc cagcgccagc aagccgaaca gctgctcagc
     1201 gccgtcggcc tggcgctgtg gctggatcag gaagcgcaga tcgatgcggt gactgccgtg
     1261 tcgggcagcg gcccggcgta tttctttctg ctgatcgaag ccatgactgc tgccggcgaa
     1321 aaactcggcc tgccgcgcga aactgctgcc cagctgaccc tgcacaccgc ccttggcgcc
     1381 gcacgcatgg ccgtagccag cgatgtcgat gccagcgaac tacgccgccg ggtaacctca
     1441 ccggccggca ccaccgaagc ggcgatcaag accttccagg ccgatggctt cgaagccctg
     1501 gtcgaaaaag ctctgaatgc cgccgcccac cgctctgccg agctggccga acaactcggt
     1561 caataaggaa cgaaaatgat cggactcaac accgccgctg tttatatcct gcaaaccatt
     1621 ggcagcctgt acctgctgat cgtgctgctg cgctttatcc tgcaactggt gcgggcagac
     1681 ttctacaacc ccgtcagcca gttcatcgta cgcgccaccc atccgctgct gaagccgctg
     1741 cgcaagatca ttccgagcct ggccgggctc gatttggcct cgctggtgct ggccatagtg
     1801 gtgcagcttg tgttgatggc gctgaccctg atgctgctcg gctatggctt ggacaatcca
     1861 ctgcagctgc tggtgtggtc gatcattggc gtcaccgcgc tgttcctcaa ggtgttcttc
     1921 tttgctctga tcatcagcgt gatcttgtcc tgggtcgccc agggcagcca caaccccacc
     1981 gccatgctga tcaatcagat ttgcgaaccg ctgctgtcac cgatccgccg catcctgccg
     2041 ggcatgggcg gactggatct gtcgcccatc gtggcgttcc tgctgctcaa cctgatcgac
     2101 atgctggtga tccgcaacct ggcgatcatg accggcatgt acaaaggcct gagcctggcg
     2161 atctgatcgc gagcgccgga tgagccactt tcgctgggac ggcgccgatc tgattctcga
     2221 gtgccatctg cagcccaaag cgagcaagga tgagttcgct gggttgcacg gtgagcggct
     2281 gaaaatccga ctcaccgcgc cgcccgtaga tggcaaggcc aatgcccatc tgcttgcctt
     2341 tctcgccagc gtgtttgcgg tcagcaaaag ccaggtcagc ctggaaagcg gcctgcaaag
     2401 ccggcaaaag cgcgtgcgca tcaaaaaacc gcagcgtctg ccaggtgaac tcgcactgac
     2461 cattcggtca ccctgactga ccataaggcg ggcggccgat gacatgcaat tgacgccata
     2521 gctgcgaccc gcataatgcc aaacaggtca atccacgaac gtcgtggatt tggcttaact
     2581 ccacaggctt taaagcgtta tttgccgcgt cagtcagctg cgctgcatcg ttcaatgtgt
     2641 gcccgactta acctgggtgc cgcatagccc cagtcgcccc acaatggatg cgtcaaccgg
     2701 aggaaagccc aggatgagca tggaacgtct cagtcagcag gtcgatgctt acgtcacctg
     2761 gaagcgcgag ctgatgcgcg aaatcacccg ctatcgcagc tggctggtga ccaatcggct
     2821 gaactccgag gccgtggaag ccaagcttga gcgcgcgctg aagctgctgc gcactgacca
     2881 catcaccctg gcctttgtcg gcgagttctc gcgcggcaag accgagctga tcaacagcct
     2941 gttcttctcc tcctacggcc agcgcatgct gccgtcccag gcgggacgca ccaccatgtg
     3001 ccccacagag ctgttcttcg acccacgctc ggagcgctcc tatatacgcc tgctgcccat
     3061 cgagacccgc gtgtccgagg ccagtgtggc gcagttcaaa cgtattccgc ggcactgggt
     3121 gaatatccct ctggacctca gcgacccgga caacatggtc caggcctttg tccaggtcgc
     3181 gcgcaccaag cccatgccgg tcgaacaggc aattgcccta ggcttccacc cggacatgct
     3241 ggaaagcacc cataaacccg gcgaagtgct ggtaccagcc tggcgtcacg ccatggtcaa
     3301 cttcgatcac ccgctgctgc gtcagggcct gcgcatcctc gacacccccg gcctgaatgc
     3361 cctcggcagc gagccggaac tgaccctgtc gatgctaccc agcgcccagg cgatcatctt
     3421 cctgctgtcc gccgacaccg gcgtaaccgc cagcgacatg gccgtgtggc agcaacatat
     3481 tcgccagctc gacgaagaca cccagaccag cctgttcgcg gtgctgaaca agatcgacgt
     3541 gctgtgggat gacctagccg gcgaagcctt tgtgcagaac gccatccgcc agatccagac
     3601 cagcactgcg cgccagctcg gcatccgggt cgaggacgtg ctgccgctgt cggccaagca
     3661 agcgatgctg gccaaggtgc gcaaggaccc ggaactgttg gcccgcagtc agatgagcaa
     3721 tctggaaaac ctgctctgcg agcggatcat cgcgcagaag gaacgcctgc tcgaagaccg
     3781 cgtggtcaat caggtgctcg cgctgctgca gaacagtcag cacgtgctca acctgcgcct
     3841 ggaaaaagtc aatgaacaac aggccctgct gagcaaccat cagcacgaca acggccagat
     3901 gctcttcgag ctgaccgcca agaccaagga agaccacagc cagcaccaca aacgcctgct
     3961 tggcctgaaa accaaccagc gcctactcaa gcgtcagggc gacctgctgc gccacgcctc
     4021 acgcagtgaa cgcctagatg aacacctgaa caaggtgcgc cgcaacctca ccggcagctg
     4081 gaccaccttg ggcatcaacc aagccatcct gcatttcttc cgcagtgtgg agcaggacct
     4141 gcacaacctg gcgcaggaag ccgagatggc caacaagatg gtcgcggcca tctaccgtcg
     4201 gcacaacgaa gagaacccgc tgcacggcgt cgacgcaccg cagttcaacg tcaaacgcta
     4261 cctgcgcgaa ctcaagcagc agcagaccaa agccgaccag ttccgcctgc agctgaaaac
     4321 cctgctgacc gaacagcgca gcctgaccaa acgattcttc gccaccctgg tacaggaagt
     4381 gatcggcctg caccaacgca tgcgccagga agccgaacag tgggccagtg atgcgctgat
     4441 gccactgatg cagcacaccc tggagcacaa gcaactgctg gaaacccaca tgctgcggct
     4501 caaagccctg gcccaggaaa cccagcaggc acgccagcgc ggccagcaac tggcgcgtta
     4561 cagcagcgaa ctggagcagc aactggcgca ggccaacgac atgctgcgca tcctgcggcg
     4621 ccctgccccc gtacaacgcc agggcaaggt ggtcaacctg ccaggggtcg cacgcccgca
     4681 tagcatcggc gaatagcgct gttggctttc actaatgaag ctcttatgct gccacgtagg
     4741 aaagatgttt aaggggtact gaaaatgaat agctcttttg acttggttgc aagccaagac
     4801 gacccgagtg acgtgcagct ttccaagcta atgtcacaag tacgtcaaac agcccacaac
     4861 aggattgctc gcgtagatag cctacttacc gaatcattgg ctcgtgcgat caaagccaag
     4921 caacagtcca ctgctgtacg cgcacagcgc aatgcctgaa gtcagaccca aactgttagt
     4981 gtttgccggc cccaatggct ccggcaagac atccatcact gagatgctgc gcaaacacca
     5041 ttggctatct gactgcacct tcgtaaatcc agatgaaatt gcgcagaatg agttcggtgg
     5101 ctggaactcg gaggacgcgc tcaagcaggc cgccgagaaa tcagagcgac tacgcgaaca
     5161 atgcctaatc gaaaagcgaa gcctggtatt tgagaccgtt ctatccactg aagacaaact
     5221 tgattttatc cagcgcgctg tagatgccgg attttttgtc agagtattct tcgtcagtac
     5281 ctcgcacccc gctatcaatg cctatcggat cacataccgc tacatggatg gaggtcatga
     5341 agtccccatc agtaaaattg tcgcgcgtta tacaaagtcc attgccaatt gcgtggcgct
     5401 gacgtcgttg gttgatcgac tttacgtcta cgacaactcg gtaaacgatc aagaccctcg
     5461 ccttctattc cgcacccgcg aagggttgct ctgcaaaaca tacgcagcgg atgacgaaac
     5521 acccgaatgg gcattgcaga tcaggcaatg cattcctagc ggaacctagc actgttcttg
     5581 ccgcctgggg cggcgctctt tagactgcgg ctcttccttt attgcgagca gggtcgatgt
     5641 cgactgcctt tccccaagac tctgttggcc tggtaacccc gcaggttctg cacttcgccg
     5701 agcctctggc gctggcctgt gggcgcagcc tggccgacta ccaactgatc ttcgaaacct
     5761 atggcgagct gaatgccacg gcgagcaacg ccgtgctgat ctgccacgcc ctctcaggcc
     5821 accaccatgc cgccggttat cacagtgcgg aagaccgtaa accgggttgg tgggacagct
     5881 gcatcggccc cggcaaaccc atcgacacca acaagttctt cgtcgtcagc ctgaacaacc
     5941 tcggcggctg caacggctca accggcccca gcagcctcaa cccggcgacc ggcaaaccct
     6001 tcggcgccga cttcccggtg atgaccgtag aagactgggt gcacagccag gcgcgattgg
     6061 ccgacaacct tggcatccag cagtgggccg ctgtgatcgg cggcagcctc ggcggcatgc
     6121 aggccatgca atggaccatc agctaccccg agcgtgtgcg gcactgcctg gcgattgcct
     6181 cggcgcccaa gttgtcagcg cagaacatcg ccttcaacga agtggcacgc caggcgattc
     6241 ttaccgaccc gcagttccat ggcgggcact tccaggaaca gggcgtgatc cccaagcgcg
     6301 gactgatgct ggcgcgcatg gtcggccaca tcacctacct gtcggatgac gccatgggcg
     6361 agaaattcgg ccgtggcctg aagagcgaaa agctcaacta cgacttccac agtgtcgagt
     6421 tccaggtaga aagctacctg cgctatcagg gtgaagagtt ctccgggcgc tttgacgcca
     6481 acacttacct gttgatgacc aaggcgttgg actacttcga tccggccgcg tccttcgatg
     6541 gcgatctggc caagacattg gcgcatgtca gcgccgattt ttgcgtgatg tccttcacca
     6601 ccgactggcg cttctcgcca gcgcgctcgc gggaaatcgt cgatgcgctg atcgctgcac
     6661 ggaaaaacgt ctgctacctg gaaatcgatg cgccccaggg ccacgacgcc ttcctgatcc
     6721 ccatcccgcg ctacctgcag gcgttcgaca gctatatgaa acggattaag gtgtagccat
     6781 gcgcgcggat ctcgaaatta tccaagagtg gattcccgca ggcagccggg tgctcgacct
     6841 gggttgcggc aatggcgaac tgctcagctg gctcagcgag cacaaaggcg tcagcggcta
     6901 tggcctggaa atcgacccgg agaatatcgc ccagtgcgtg agcaagggcg tcaacgtcat
     6961 cgagcagaac ctcgacaagg gcctgggtaa cttcgccagt aacagctttg acgtggtggt
     7021 gatgacccag tcgctgcagg ccctgcatta cccggacaag gtgctcgcgg aaatgctgcg
     7081 catcggcaag acctgcatca tcaccttccc caacttcgcc cactggcgct gccgctggta
     7141 cctcaccacc aagggccgaa tgccggtgtc ggactttctg ccctacacct ggttcaacac
     7201 gccaaacatc cacttctgca ccttcgaaga tttcgaaaag ctgtgccacg accagggcgc
     7261 aaaagtcatg gatcgtctgg ccgtagaccg cgaccaccgg cacggctggg ccagccggat
     7321 ctggcctaac ctgttaggtg agattggcat ttaccggatc agcgggccta ctgccgaaga
     7381 cgtgcagatc gctaactaac acgccgtagc aaccgcttct ggagaaccct tatgcgtcgc
     7441 atagcattat tgtttatcgc cctgtgcctg agcctgccgg cgctggctga acgcaagcaa
     7501 accttcggcg atctcgatgt gcactacatc gccttcaact ccggcttttt gcagccggat
     7561 atcgccgcgg ccagcggcct ggtgcgcagc aagacgcagg gcgtggtgaa tatctccgtg
     7621 ctcaaggcgg gcaaggccag cagcgccagc gtcagtggtg aagtgaaaga tctggtcggc
     7681 cgcagccagc cgctgacctt caagcaggtc accgaaggtg aggccatcta ttacctggcg
     7741 caattcaact tcacccagcg cgagatgctg cgtttcacca tcaatgtgca tgctaccgat
     7801 ggcgtggcgc acagcttcaa tttcaaccag gaagtcttcc cagacgaatg atggccctga
     7861 atccatgatg catttcaacg agcttgtact cgccagccat aacgccggca aactcaaaga
     7921 gctgcaggcc atgcttggca gcagcgtacg cgtgcgctcg gtcggcgaat tctccatggt
     7981 cgagccggaa gaaaccggcc tgtcgtttat cgagaatgcc atcctcaagg cacgcaatgc
     8041 cgcgcggctg tccggcctgc cggcgctggc cgatgactcc ggcctggcgg tggattttct
     8101 cggcggtgcg ccgggcatct actcggcacg ctacgcagac ggccagggcg atgcggcgaa
     8161 caacgccaag ctactcgacg ccctgaaaga cgtaccggat gccgagcgcg gcgcgcagtt
     8221 cgtctgctgc ctggccctgg tgcgccacgc cgacgaccca ctgccgatca tctgcgaagg
     8281 gctctggcac gggagcatcc tgcatgccgc gcaaggcgtg gaaggctttg gttatgaccc
     8341 gctgttctgg gtgcccgagc gcgactgctc cagcgccgag ctgccggcgg ccgagaaaaa
     8401 ccagatcagc caccgcgccc gcgccatggc cctgctgaaa cagcggctgg gcctggcatg
     8461 accacggcgg ccggcggcgg cttcgagctg ccgccactcg cactctatgt gcacatcccc
     8521 tggtgcgtga agaagtgccc gtattgcgac ttcaactccc acgccgccgg gccaacgctg
     8581 ccggaagaag aatacgtcga cgccctgctg gcggatctgg atgctgatct ggcgcaggtg
     8641 cataaccgac cgttgagttc gatcttcttc ggcggcggca cgcccagcct gttttcggcc
     8701 aaggccctcg gccgtctgct tgaaggcgtg gagcggcgcg tgccgtttgc cgccgatatc
     8761 gaaatcaccc tggaagccaa ccccggcacc ttcgagcagg ccaagttcaa ggactaccgc
     8821 agcctcggca ttaatcgcct gtcgattggc gtgcagagtt tccaggcgcc caaactgatc
     8881 gccctggggc gcatccacga tggcgatgaa gccgtgcacg cagccgatat ggcccgcgcc
     8941 gccggcttcg acaatttcaa cctcgacctg atgcacggcc tgccggacca gtccatcgaa
     9001 gacgccctca gcgatctgcg tatcgccatc gcccaggggc cgacgcatct gtcctggtat
     9061 cagctgacgg tggagccgaa cacggtgttc tggaaccagc ctccagtgat gcccgaggac
     9121 gatattctct gggacattca ggaagccggc caggccctgc tggccgccga aggctacgcg
     9181 cagtatgaag tctcggccta cgctcaggcc ggcaaggccg cgcggcataa cctcaactac
     9241 tggacctttg gcgatttcct cggcatcggt gccggcgctc acgccaagct gagccacccg
     9301 gacggcaaca tcagtcgcag ctggaaaacc cgcctgccca aggactacct ggacagcagc
     9361 aaacgcttca gcgccggcga gcgcccgttg agcgcagatg aattgccctt cgaatttctg
     9421 atgaatgtgc tgcgcctcac ggacggcgtc gccagcgaac tgttcactca gcgtaccggt
     9481 ctgccgctca gccagcttgc tgcggcgcgc gccgaggccg agcaacgcgg cctgctgcac
     9541 agtgacccga cacgcctgag cgccacccgc gaaggccagc tgtttctcaa cgatctgctg
     9601 caacactttc taccctgacg tacacgccgc cacggctaag gaaaccggat ggatctgcta
     9661 ctcgacctga tcgtcaccct gtcgcgctgg agccgcagcc acctctacga catctccctg
     9721 gcgatcatgg ccaccctact ggtgctgttc ggccctggca tcaacgcctg ggtgcagcgc
     9781 aataccagca gcatgaactt catcttccgc acgctgatct tcgtgctgct ctgcgcggtg
     9841 ggctacggcc tgctgatcgt cttcgccacg ccgtacctgg ccaaggggct gggctttttc
     9901 aacaacttca ccttggctcc agtgctgatt ctggtgttct ttatcatcgg catcctcgcc
     9961 gaccgcagct agatcgatac tcaagagaat ggtttgatcg ctaccttgag cacgccgtcg
    10021 cgctggtgag cgaatagatc ataggctgcg acgatgtcat ccagcgaata ctggtgcgtc
    10081 accagcgcgc cgaaatcaac gcgcccggag gccaccacat tgatcagccg gcgcatgcgc
    10141 tccttgccgc cagggcacag cgaggtgacg atcttgtggt cacccaagcc ggcatggaaa
    10201 gcgcccagcg ggatcaccag atccgttgaa tacaccccca ggctggacag cgtgccaccc
    10261 ggtttgagca cccgcagcgc actttcgaag gtggattgca ggcccaatgc ctcaatggcc
    10321 gcatccacac cacggccccc ggtcaggcgt agaatctcct caaccacatc gacctgattg
    10381 aagttgagag taacgtccgc acccatcttg cgcgctatct gcaggcgctg ctcgacgcca
    10441 tcgaccacga tgatggtgct cgccccgcgc aacttggcac cggccgtggc gcacagccca
    10501 atcggcccct gggcgaagac tgcgaccaca tcaccgatct tgatattggc cgcttccgcg
    10561 ccggcaaacc cggtagacat gatgtccgga cacatcagca cctgctcgtc agtcaggcca
    10621 tccggcaccg gggccaggtt gccctgggcg tcgggcacca acacatactc ggcctgggtg
    10681 ccgtcgatgc tgttgccaaa gcgccaaccg cccatgggtt tatagccatg gcagctgcag
    10741 ccgccatcct gggaggaata accgtcctga ctggcgtagg agctgaagct tgggcagatg
    10801 gctccggcga tcacccgctg cccttcttga taaccctgta cgttgctgcc cagcttctcg
    10861 atgatgccaa ccggttcgtg gccgatggtc agccccttgg ccaccgggta ttcacccttg
    10921 aggatgtgca catcggtgcc gcagatggtc gtggtggtaa tccgtaccag tgcatcattg
    10981 ggccccactg gcgggatcgg tttgtccacc agttcgatgc gtccaggctc gataaacacc
    11041 gctgctttca tcatgctcat ggtcaggctc cttgtatgcg ctagcacaga gctctgaatg
    11101 tagtcgggtg actgcccggc atcttgtcct gcatcaatgc caggcacagt cagagcaagg
    11161 ctccaaagcc gcagaccagc gatcaccaag cataaaaaaa ccgcctgcaa gaggcggttt
    11221 tcagtactac cgaggctcag ttgccttcgc gtcgtagcgc ctgcggggtg aaatcgcgcg
    11281 gtgtcagctt ggcgttgaag ttgtacatcg gttcgttgtt atccaggcca tcgacgaagt
    11341 agcgcttgcc cttgaagtcg tagatggtct ccagagtgct gccaaacatc ggcacgtcgt
    11401 agtagctgat cgggtggctt tcctgcacac cgctcagctc gccgctcttg tcgtagagat
    11461 cggcggcgag gatctgccag ctgtcctcgt cgatgtagaa acggcgcttg gcatacggat
    11521 ggctaaagcc cttgcgcaac tcggcatcaa ccacccagac gcggtgcagc tcataacgca
    11581 gcagttccgg gttgacggtg ttgctgcgca ggatgttgtc gtaggggatg cccttctggt
    11641 gcacggcgta actgttatac ggcacgagca tttcctgctt gccgaccagc gtccactcgt
    11701 aacgatccgg cgcgccattg taggagtcaa ccacgtcggc ggtggccatg ccgttgctgt
    11761 ccggttgcag ggtgtcgtag gccagcatcg gcaaacggcg cacacggcgc tcgccacggt
    11821 tgaagcgcca ggccttgcga atcgccagta cctggtcgag ggtttcctgc accaccagcg
    11881 ccgaaccggc gagcttggcc ggcgcgacga ccttgtactt gtagtagaac agggtgttgt
    11941 ccaggtcctg cgggctgacg ccttcacggc cgtagaggaa atagatatcg cgttccagct
    12001 tgagcaggtt gtaggcgccg ttggacagca ctgcagcctg gttggtgacg aagctgatct
    12061 ggtcgccacg gtaacgcatg atgtggttcc agatggcttc ctggccggtc ttcggcagcg
    12121 ggaaggggat gccggcggct gcaccatcaa caccattacc gccggaaatc agctcggcag
    12181 tctgcgcgtt gaagcgcgta gcgtcataga tacgctgcgg cgctgcagcg ctgcgacggg
    12241 tcgggaatac ccgcagaaag taatccggat ttttttccag caacgccttg aggcccaccg
    12301 gcagctgcgc ctggtactgc gccaggttct ggctattgac ctggtacaag ggcgcatcgg
    12361 cgctgtaagg gtcagggtgg tgcatgcccg gctggtagtt ggccggcggt tgtgcaaggc
    12421 caccatccca ggctggaatg gtgccggcgg cattgccggc gcgctcaccg cccagcggcg
    12481 tcaggtcctg gcccagacgt gcagcttggg tgctgtcgac tttggcctgt gcctgtaatg
    12541 cgcacgtagc cagcagcaga attgaaagcg ttctcaacac agcgatctcc tcgcagcctg
    12601 gccaatgcca gggctgctgt ttttatgacc ggggcaatgg tgcccggttt tatatgccgt
    12661 cttggtgagg agccgtcctg gcgggcgccg gattattccg atcttgtgca atcgtccctg
    12721 tgcttatggt tttgctgcgc cgatagctca gcgcgtgaat caatcagtcg atgcgctgga
    12781 acttcagatc ccatacgcca tgccccagac gctcgccgcg gcgctcgaac ttggtggtcg
    12841 ggcgctccgt cgggcgcggc acatactggc catcgtcggc ctggttctgg taaccgggcg
    12901 ccacgttcat cactcccagc atgtactcgg cgtagggctg ccaatcggtg gccatgtgca
    12961 atacaccgcc aaccttgagt ttctgtcgca ccagctctgc aaacgccggc tgcacgatac
    13021 gccgcttgtt gtgcttggcc ttatgccagg ggtccgggaa gaatagcagc agccgatcca
    13081 ggctggcgtc ggccacacag ttgcgcagta cgtccatcgc atcgcagctg tagacgcgca
    13141 tattactgag gttctgcgtc atcaggccgt tgagcagcgc gccgacgcct gggcgatgca
    13201 cttccacccc gataaagtcc tgctcgggcg aagcggcagc catttccagg gtggaatggc
    13261 ccatgccgaa gccgatttca aaagtacgcg gcgcactgcg accgaatacc tgatcgaagt
    13321 cgcgcaggcc atcctccagc tgcaggccga acttcggcca gccctgatca aggccacgtt
    13381 gctggccttc ggtcatacgc ccggcccgca tcacaaagct cttgatcgcg cggtgcttgg
    13441 aggtgtcttc agccggctca ggctgttgct gcaattcggt catgctgtgc tcttaaagtg
    13501 cttttactta atcaggccat ccagcggcga agaggcgctg gcatagagtt ttttcggcat
    13561 gcgcccggcc aggtaggcca tgcgtccggc gatgatcgcg tgcttcatcg cctcgcccat
    13621 cagaatgggg ttctgcgcat gggcaatggc gctgttcatc agcaccgctt cgcagcccaa
    13681 ctccatggca atcgtggcat cggacgcggt gccgacaccg gcatcgacca ataccggaac
    13741 ggtagcttct tcgaggatga tgcgcaggtt gtaggggttg cagatgccca ggccagtgcc
    13801 gatcagcccg gccagtggca tcaccgcgat gcagcccatg gccgccagtt cacgggcgat
    13861 gatcgggtca tcgctggtgt acaccatcac gtcgaaacca tccttgacca gcacttcggc
    13921 ggccttgagg gtttcgatca cattggggaa cagggtcttc tggtcggcca gcacttccag
    13981 cttgaccagc ttgtggccgt cgagcagctc gcgggccagg cggcaggtac gcactgcctc
    14041 aacggcgtcg tagcagccag cggtatttgg caggatggtg tatttgtccg gcgagatcac
    14101 atccagcagg ttcggctcgc ccggattttg cccaatattg gttcgacgca ccgccacggt
    14161 aacgatctcg gcacccgagg cctcgatggc caggcgggtt tcctcaagat ccttgtactt
    14221 gccggtaccg accagcagac gcgactggta agtacgaccg gccagggtaa agggtttgtc
    14281 gctgggcgca tggctcatgg gtaatcctcg taaagtgggc ggactttcag cggaatcgac
    14341 tggccttaac cgccaccgat ggcgtgaacc acttcaacct ggtcaccgtc gcgcaaggcg
    14401 gtggttgcgt gctggctgcg cggcacgata tccagattga gctcaaccgc cacgcggcgt
    14461 ccggccagat ccagacggtc aatcaacccg gcgacagttg taccgtcggg caattcgaat
    14521 gcttcaccgt ttaactgaat atgcatgacg ggtcggtcac aactgggaaa agggccggca
    14581 ttctagcctg atcataggcg ttgaccaagg ctgaaggctg ccgctcgtcc tgctttctga
    14641 cttacgggtc aggcgatacc gcgcgcaccc agcttccagg ccaccatacc caggcacagc
    14701 cagccggcga gaaacgccag accgccgaaa ggcgtgacca tgcccagcgc actgatgccg
    14761 ctcaaggtca gcgcatacag gctaccggag aacagcacga tgcctagagc aaacagcgcg
    14821 ccggcgcaat tgaccagcgg cgcaggccgt agcatggcca gcaggccaac gccgaacagc
    14881 gccagcgcgt gaatcagctg gtagtgagtg ccagtctgaa ataccgcgag ataagccggt
    14941 gtcagctgac ttttcaggcc atgcgcggca aaggcaccca aggcgacacc ggtaaaaccg
    15001 gcgcaggcgg acagcaataa ccacagacga gccatgcatc actccagata taaagacagg
    15061 gctggctata atggccggca aaatcaccct ggccaagcac gcatgtttcg atccatcacc
    15121 cgtcgcctgc tcaaactcct gctctggctg atcctaggct cggtattgct ggtgttgttg
    15181 tttcgctggg tgccgccacc cggcacggcg ctgatggtcg agcgtaagat cgaatcctgg
    15241 ctcgacaacc ggcccatcga cctgcagcgc agctggcgcc cctggagcga gctgccggat
    15301 gatctgaaga tggcggtgat tgccggcgaa gatcagaagt tcgccgaaca ctggggcttc
    15361 gatgtcgcgg ctatccaggc agccctggag cacaaccagc aaggcggttc cctgcgcggc
    15421 gccagcaccc tcagccagca ggtggcgaag aatctgtttc tctggtccgg tcgcagctgg
    15481 ctgcgcaagg gtatcgaggt gtggttcacc gggctgatcg aactgctctg gcccaagcag
    15541 cggattcttg aggtgtacct caacagcgtc gaatggggcg acggtgtgtt cggcgccgag
    15601 gcggcggcgc ggcatcactt caaggtcggc gcgccctacc tgtcacgcca gcagagcagc
    15661 ctgctggccg ccgtgctgcc caacccgcgc gagtggagcg ccagccgccc gagcgcgcat
    15721 atcagccgtc gggccaactg gattcgccag cagatgtggc aactgggtgg cagccattac
    15781 ctcgatcgcc tgcaacccag ccgtcctgaa tggtggccaa gctggttaaa ggacgtttag
    15841 ctcagcgctg tttaagcaga tgccgcggca cagcatagag gaagtacagc tcttcatctt
    15901 gcaagtgcgc ggtcatgccc agctcgggat agacccaagc ctgcccctcg gccagctcca
    15961 gagtcagacg cggctgaccg atgctcgcgg ccagtcgcgc aacatccagc ggcgccggcg
    16021 gtagcaaggt cagcgcaata atcgggcgat tggccaatgg cactaacaac tggctaccca
    16081 gcggctgctc cttatccgtc ggctttaggc cggcggcggc cagcaagctt tcgcgctcgg
    16141 ctttgtccag accgacttca gcctccagcg accaattgcc atcagcatcc tgcagctcgc
    16201 cgcgatacag cagacgtgca ccgtccagat tcggctgcag ccaaagctcg ccctccagag
    16261 catccttcag atgcaccagg ctcaagcgat catcggccag cggcgtaagc accttggctt
    16321 gccattctgc cagccaaggc aagggttgcg cggccggctg cgggcgcatc agccacgcgc
    16381 cccaggcaaa gaacaaggcg gcgaccacgg caaagatcag ccagtgctgc gggcgtatgg
    16441 gcggtaattt cacgggcttt tccttaggca gaaaaaagcc gcacctgggt gcggcttctg
    16501 acagtagcgc gatttacgcg gcgattgagc ccttgagctt gttcatcgcg ttcttttcca
    16561 gttgccggat gcgctcggcc gatacgttgt acttggcggc caggtcatgc agcgtggctt
    16621 tctcctcggc cagccagcgc tggtagagga tgtcacgact gcgctcatcc agaccttcca
    16681 acgcttcgtg cagattagcg gtggagctgt cgctccagtc ggaatcttcc agctgacgcg
    16741 ccgggtcgta gcggtggtct tccagataat gcgcagggga ctggaaggcg ctttcgtcgt
    16801 cggcttcggc agccgggtcg aaggccatgt catggccggt caggcggctt tccatctcgc
    16861 gcacttcacg cggctcaacg cccaggcttg cggccacggc atgcacttcg tcgttgttca
    16921 accaggccag acgcttcttc tggctgcgca ggttgaaaaa tagcttgcgc tgcgccttag
    16981 tggtggcgac tttgacgatg cgccagtttt tcaggatgaa ctcgtggatc tcggcgcgaa
    17041 tccagtgcac ggcgaacgac accaggcgca cacccatttc cgggttgaag cgcttgaccg
    17101 ccttcatcag gccgacgttg ccttcctgaa tcaggtcagc ctgggccagg ccataaccgg
    17161 agtagctgcg ggcgatatgc acaacaaaac gcaggtgagc cagcaccatt tgccgggcgg
    17221 cttcgaggtc ttgtttgtag aagagacttt ccgccagctc acgctcctgc tcgggggtca
    17281 acagcgggat gccgtttacc gcatgcacgt atgcctccag gttggcaccc ggaaccagag
    17341 catgaacagg ttgcaaagaa gtggacattt gaatcctccg acttacgaat cgtggagcag
    17401 tctagcattg ctccttggga ctttataccg aggtacaagt tcccttacac atagtcaata
    17461 tcaagcaaaa caataactta ctatctaggg gccagctcgc tcaagtgcct tgcgaccgcc
    17521 agccaggcgc caatatagcc caacaacacc gcgccgagca gcagggatag accatcggct
    17581 atcggcacgc ccgccagggc gaagtcgctg ccgtacaaac ccgccaggcg caccacggca
    17641 tcattcagcc agccgaggcc gtaggccagc agcagccagg ccagcaggcc ggcgcccaaa
    17701 ccgtacagcg tgcccatata gaggaagggc cgacgcacat agctgtcggt gccgccaacc
    17761 agcttgatca cctcgatctc ggtacggcgg ttctcgatat gcagacgaat ggtattgccg
    17821 atcaccagca gcagcgccat cacaagaagc aaggtcaagc caaagacgaa acgctcaccg
    17881 agcttgagca tggcgctcag acgctcgacc cagagcagat caagctgcgc ctgctgcacg
    17941 cgcggcagct cggccaggcg caagcgcaat gcttccaagg cgaccttgtc gacttgctgc
    18001 ggcgtcacca cgatcacccc aggcagcggg ttatccggta gttctttcag cgcttcgccg
    18061 aggccggatt gctgctgaaa ctcgtccaga gcctgttcac ggctgatcca ttcggcctct
    18121 tccacatcgg ccatctcggc gatctgctcg cgtagggcct ggccctgagc atccgtagcg
    18181 tcgatttgca aaaacaggga aatctgcgcc gcgcgctgcc aagacacacc caggcgctcg
    18241 acgttatcca gcagtagcgc caagcccatg ggcaagctca gagccacggc catcaccagg
    18301 caggtaaaga agctgccaat cggctgcttg cccaatcggc gcaggctgtc gaccaagctg
    18361 gcacggtggt tttccaacca ggcatgcagc tgatgggtaa agcttgggcc atcgtcgccc
    18421 ggagtcggcg attcggattt tttcggcgcg gcaccgacac gctgtgcggg ctgcggaggc
    18481 ggcatgcggg tggcgctcat gcagcctccc cgtcgccgat caggcggccg cgttgcaggg
    18541 tcagcatgcg gtggcgcatg cgcgcgatca gcgccaggtc gtggctggcg atcagcacag
    18601 tggtgcccag gcggttgatg tcttcaaaca cccccatgat ctcggccgcc aggcgcggat
    18661 cgaggttacc ggtgggttca tccgccagca gcagcgccgg gcgatgcacg acggcgcggg
    18721 caataccgac gcgctgctgc tgaccggtag acaggtcgcc aggggattgc tcggccttgt
    18781 cggccagcga cacacgctcc agcgcagtgc gcacgcgagt gccgatctcg tccttggaca
    18841 agccgagaat ctgcagcggc agcgccacgt tgtcgaatac gctacggtcg aacagcagct
    18901 gatggttctg gaacaccacg ccgatctgcc gacgcaggaa tggaatctgg gcattggtga
    18961 tgtgcgacag gtcctgcccg gccagcaaca gcttgccact gctcggccgc tccatcgcca
    19021 gcagcaggcg cagcaaggtg ctcttgccgg cgccggagtg cccggtaacg aagaggaact
    19081 cgccacggcg cacgtggaaa ctcagttcgt gcagcccgac gtggccgttg gggtagcgtt
    19141 taccgacctg ctcgaatttg atcatcctat ttctccgcga acagcgcctg aacgaaggct
    19201 tcagcctcga aggtgcgcag gtcatcaatg ccctcgccca ccccgatata acgaatcggc
    19261 aaagcaaact gcttggccag ggcaaagatc accccgccct tggcggtgcc gtccagcttg
    19321 gtcagcgcca ggccggtgag gttgaccgtc tgattgaact gcttagcctg attgatcgcg
    19381 ttctgcccgg tgccggcgtc cagcaccagc agcacttcgt gcggcgcggt gtcatccagc
    19441 ttgccgatca cccggcggac tttcttcagt tcttccatca ggttgtcttt ggtgtgcaga
    19501 cgcccagcgg tgtcggcgat caatacgtcc atgccgcgcg atttggccgc ctgtaccgca
    19561 tcgaagatca ccgaggccga atcagcgccg gtgtgctggg cgattactgc aatctgattg
    19621 cgctcgcccc acacctgcag ctgctcaacg gcggcggcgc ggaaggtgtc gccggcggca
    19681 agcatgactt tcttgccatc ctgctggagt tttttcgcca gtttgccgat ggtggtggtc
    19741 ttgcccgcgc cgtttacgcc gaccaccaga atcacatagg gctgcttgct gctgtcgatc
    19801 tgcagaggct gctcgaccgg cttgagcagc gccaccagct catcctgcag ggccttgtac
    19861 aacgcgccgc tgtcagccag ctccttgcgt gccacacgtt tggtcaggtt gccgatgatc
    19921 gccgtggtgg cctcgacgcc aacgtcagcg gtcagcaaac gggtttccag gtcttcgagc
    19981 aggtcatcgt cgatggcctt cttgccgaga aacaggctgg ccatgccctc gccgaggctg
    20041 gcgctggttt tcgacaggcc ttgcttgagg cgttcgagaa agccggcacg ctgttcttcg
    20101 gcacttttaa ccggcgcagc ggctggctgt acgactgctg gcgcggccac tggaggctcg
    20161 acgacaggct caaccaccgc aggcacttca atgtgcgcat gcagcacttc gctgccggca
    20221 ttgatgcgga aacgcgacgg gcgtggctgc gggttggctt cgctgccttc agccggcgcg
    20281 ggaatggcgg cagcggcctg ctcaaccgga gcgaccggaa caggttgcgg ctcgacggca
    20341 atggcgggcg attcaactgg cgccgcaact tcgcttaccg gcgcaactgg ctctgatgcg
    20401 ggcgtggcga cctcggtagc cggagcagct ggctgctcga taaccggagt cagttgtggc
    20461 tcaggactgc tcggcgcttg cggcttcttg cgcagccagc cgaacaggtc tttcttctct
    20521 gcaggtttct cggcagaggc cggcgtagag ggcgcgggcg tgttcttgtc gtcgttggaa
    20581 ccaaacatgg aggacggcta tctcaaaggt gcgacgcgca ggccacaggc tctaaaaagc
    20641 attactcacg cgacattaaa agctgaagag tgcacgcggt aacaatcaga agccttagca
    20701 ggcgtggtcg ccagtaaaac ggatgcgtat cctagcacct ccgcgcccgc cgacgctaag
    20761 accaagcggg cccgtccgac aggtcaataa tccatgcata tgattgccca acgcactgca
    20821 gctctgctgc tcggcgtgct ttgtacaccg ctgctggctc tggccgcaga aacccaaccc
    20881 acccacgaat tcagcctgga caatggcctc aaggtcatcg tgcgcgaaga ccatcgcgcc
    20941 ccggtggtgg tcagccagat ttggtacaag gtcggctcca gctacgagac gcccggccag
    21001 accggcctgt cccacgccct cgaacacatg atgttcaagg gcagccgcaa actcggcccc
    21061 ggcgaagcct cgcgcattct gcgcgagctg ggcgccgaag aaaacgcctt taccagcgac
    21121 gattacaccg cctactacca agtgctggcc cgcgaccgcc tggccgtagc cctggagctg
    21181 gaagccgacc gtctggccag cctaaagctg ccggccgatg agttcgccaa ggaaatcgag
    21241 gtaataaagg aagagcgccg cctgcgcacc gacgacaaac cgtcgagcct ggcctacgag
    21301 cgcttcaagg ccatggccta ccctgccagc ggttaccaca cacccaccat cggctggatg
    21361 gccgacctcg aacgcatgac cgtcgaagag ctgcgcgact ggtacgaggc ttggtacgcg
    21421 ccaaacaacg ccaccctggt agtggtcggc gatgtcagcg tcgccgaggt gaaaactctc
    21481 gccgagcgct atttcggccc gatcctcaag cgcaccgtgc cgacggcgaa agccccgcgt
    21541 gagttggccg caccgggcga gcgtcgcatc accctgcacc tgaaaaccca gctgccaagc
    21601 ctgatgatgg gcttcaacgt acctggcctg gccactgccg aacaaccgcg tcaggtccat
    21661 gctttgcgtc tggccgctgc gctgctcgat ggcggctaca gcgctcgcct gccgacccgc
    21721 ctggaacgtg gcgaggaact ggtgtccggc gcatcggcct ggtatgacgg gttctcccgt
    21781 ggcgacagct tgttcatcct ttccgccacg cctaacgtgc agaccggcaa gaccttggat
    21841 caggtcgaag ccggcctgtg gcgtgagctg gaggatctga aaaacacccc gccaagcgcc
    21901 gaagagctgg cccgcgtgcg tgcacaagtg atcgctggtc tggtctacga gcgtgactcg
    21961 atcaccagcc aggctaccgc catcggccaa ctggaaaccg tcggcctgtc gtggcagttg
    22021 atcgacaagg aactggccga actggaggcc gtgacaccgg ccgatatcca gcaagccgcc
    22081 agcaccttct ttacccgcga ccgcctaagc gtcgcccatg ttctgcctga ggagaaacgc
    22141 aatgagtgag cgcaatggcc tgcgctacgg tctgctcggc ctggcgctac tgatagtgct
    22201 ggcgctgctg gccctggtga tcaaccgccc ggacagcagc ccccccccgg ctccggccgc
    22261 cgaatcgacc agcatcgaat ccctggccgc ccttggcgat cagccaccga gccgccgcga
    22321 cctgaatatc cagacctggc agactgccga aggcgccaag gtgctgttcg tcgaagcccg
    22381 cgagctgccg atgttcgacc tgcaactgac ctttgccgcc ggcagcagcc atgatgatgg
    22441 cgtaccgggc ctggccatgc tgaccaacgc catgctcaac gaaggcgtac cgggcaagga
    22501 tgtcggcgca atcgccgccg gcttcgaaaa cctcggcgcc aacttcggca acggcgcgtt
    22561 tcgcgacatg gccatcgcca gcctgcgcag cctcagcgct gcggacaaac gcgaaccggc
    22621 catgcaactg ttcaatcagg tgctcggcca gccgaccttc ccggaagacg ccctagcgcg
    22681 gatcaagaac cagctgctgg ccggcttcga ataccagaag cagaaccccg gcaagctggc
    22741 cagcctggcg ctgttcgagc gcctgtacgg cacacatcct tacgcgcacc cgagcgacgg
    22801 caatgccgag tcgatcccaa gcatcagccg tgagcagttg caggctttcc acgctcaggc
    22861 ctacacagcc aataacgcga ttatcgcgct ggtcggcgac ctctctcgca gtgatgccga
    22921 agccctggcg agccaggtat ccgccgccct gccgaaaggc ccggcgctgc cgcgtatcgc
    22981 ccagccggaa gagcccaagc cgggcctgca acatatcgag ttcccctcga atcagaccca
    23041 cctgatgatt gcccagctcg gcatcgaccg ccgcgacccg gactacgccg cgctctacct
    23101 gggcaaccag gtattcggcg gcggtggttt cggcacccgc ctgatgaccg aagtacgcga
    23161 aaagcgcggc ctgacctatg gcgtctactc tggcttcagc gccatgcagg cgcgtggccc
    23221 ctttatgatc aacctgcaga cccgtgccga actcagcgac ggcaccctgc aactggtcaa
    23281 aagcatgctc gccgacttta tcgcctacgg cccgaccact gatgagctga acaatgccaa
    23341 gcgcgagctg gccggcagct tcccgctgtc caccgcgagc aacgcggcga ttgtcggcca
    23401 actcggctcc atgggtttct atgacctgcc gctgagctac ctggaagact ttatgcgcga
    23461 cgtcgaagcg ctgagcgtgg aacaggtcaa agccgccatg gccaagcacc tgaatcccga
    23521 cgccctggtg atcgtcaccg ccggcccgag cgtgacgcag aaagaactgc caccgcccac
    23581 cgaccgcccc gccgaacaac cttctggcgt accggagcac tgatgagcaa gcgacccaaa
    23641 ccgccaaaag ccaaccccgc ccacagtggc gatggccagc tgcggatcat cggcgggcaa
    23701 tggcgctcgc gccagttcaa cttccacatg gctgccgggc tgcggccgac gccgaaccgg
    23761 gtgcgcgaga ccctgttcaa ctggctggcg ccctatgtcg agggcgccaa ggtgctcgac
    23821 ctgttcgccg gcagcggcgc gctgtttctc gaagccctgt cgcgcggcgc tggcaacgcc
    23881 ctggccctgg acctcaaccc agcggctatc gccagcctgc gcagccacct gcaaacgctg
    23941 aagtgcgaca acggccaact gcagcagacc gacgccctgc tctttctgca aaacccagcc
    24001 gtcacgccgt tcgatctggt gtttctcgac cctccgttca accagaacct gctgctgccc
    24061 gcctgcaccc tgctcgagca gaatggctgg ctggcggccg acgcctgggt ctacaccgaa
    24121 agcgagaatt caccgtccag cctcggcatg cccggcaact ggcgtctgca ccgtgagcag
    24181 aaggccggcc aggtgtacta cgcgctatgg cagcgcacgg ctgcagcctg aggcaaactc
    24241 cgggcttcac gtcgtgaagc ccaacctccg tcaggctctg ctattcgccc cactcatata
    24301 gcttccgttt caccgcctca ggccctagcc tgagcgcttt cccaagcgat acctgtttat
    24361 gaccacgccc ttcacacccg cctggtggct gcccggccca catctgcaaa cactgtggaa
    24421 cccgatgtgt cgcaagccgg cgaagcttgc gcgcacacgc gagcggctgt ggctggacga
    24481 tggcgacttt cttgatctgg actggcacgg cccacaccag gccgacgcgc cgctggtgtt
    24541 ggtgctgcat ggcctgacgg gctcgtccga ctcactgtat gtgctgggtt tgcagcaaga
    24601 actggcggcc cagggctggg cgagcgtggc gctgaactgg cgcggctgct ccggcgagcc
    24661 gaacctgctg gcccgtggtt accactccgg cgccagcgag gacctggcag aaaccgtgcg
    24721 ccacctgcgc gcccaacggc cgatggcgcc gatctatgcc gtgggttatt ccctgggcgg
    24781 taatgtgctg ctcaagtacc ttggcgagag cggcgccgac agtcagctgc agggcgcagc
    24841 tgcggtgtcg gtgccgtttc gtctggatca gtgcgccgac cgcatcggca tgggcttttc
    24901 cagggtctac cagagccact ttatgcgcga gatggtggcc tacgtgaagg acaagcagcg
    24961 cctgtttgcc caccagggca tggccgaccg cctatccacc ctggagaagc tcggctcgct
    25021 ggacaatatg cgcaccttct gggatttcga cggccgcatc accgcaccgt tgcatggcta
    25081 cgccgatgcc gaggactact accgccgcgc ctccagccgc tacttcctcg gccagatcga
    25141 gacgccgacg ctgattatcc aggccgccga cgatcccttc gtctttaccc acagcctgcc
    25201 cgaagccagt gagctgtcac ccagcatcga attcgaactg cacgcccacg gtggccatgt
    25261 cggctttgtc gaaggcagcc tgggtaaacc gggttattac ctggaacggc gtattccgca
    25321 gtggctgaaa caacaattca acccgtagcc cggattgcgc caaggcttat ccccgggcta
    25381 cggacttgac ggctcgcgct taaatcacct gttccagcgg cacatgcagc ttggcgtaca
    25441 acgcctcgac cgcctcacgt gcactggggg tgatgtagcc gccctccagc gccagcacct
    25501 gataaatgcc acggcgcagc atctcctggc tgaggtcgcc ggggttgccg cgagtggtgc
    25561 agagaaagcg cacccaggag gtcatgatga tccagctgtt aagggtcagc gcctcaatct
    25621 gtggcgcatt catcaacagg atgccggcct cgacaaagcc ctgatagatg ttctgcgctc
    25681 gctgcaggca gcgctgcgag aacagcttgt agcgctcagc cagctcggcg tcgctgtcga
    25741 gcaggtgctc aagatcgcgg tgcagaaagc ggtagtgcca catggcggcg agcagtgctt
    25801 ccagatagaa ggtcttgtcc tgcaccgtca gcgcgcgacc ctcggggcga cgtaaaaagg
    25861 tatccacctg gctttcataa agggtaaaca gctcggcgat gatcgcctgc ttgttacgga
    25921 agtggtagta caggttaccc ggtgaaatcc ccaggtgtgc ggcgatatgg ttggtggtaa
    25981 cgctgcgctc gccctgggcg ttaaacagcg ccaggctctc ctgaatgatg cgttcgcggg
    26041 ttttcagtgg ggctgacata acgactcgat ctcggctcaa aagctcggca aaggtacagg
    26101 cagaaacgcc agaagtgtta ccggaaaaag ccgtggaaac atgcgctaac ttttcgttgg
    26161 gctgcgtctg tcggtggacc aggccatgct atttgacaga ttagagcaat tgctctaaaa
    26221 atccagcaca ccttttctgc agcggcctca gctcatctga tgcactgcat tgctggaatg
    26281 ccgaggagca acaccatggt cgccgacgtc gcttacctag aacaaagcct gcaacgcagc
    26341 caacagcagc tcagccaact ggagagcagc tttacccgtc agcgccaagc gttcaaggcc
    26401 aaccccatgc cttcggccga acaacgcatt cagtggctga acgtactgcg cgacctgctc
    26461 agcaacgaac gtgatgcgct gatcagcgcc atctcccagg atttcagtaa ccgcagcgcc
    26521 gacgaaaccc tgctcgccga gctgatgccg agcctgcatg gcattcacta cgcgaccaaa
    26581 cgcatcaaga agtggatgaa gccttcacgg cgcagcgtcg gcatgcaatt tatgccggcc
    26641 gccgccaagg tcatctacca gccgctgggc gtggtcggag tgatcgtgcc gtggaactac
    26701 ccgctgtttc tcgccattgg tccgctggtc ggcgcgctgt cagccggcaa ccgggtgatg
    26761 atcaagatga gcgagtcgac cccggccacc tcgcaactga tcaaggacct gctggccaag
    26821 gtcttccccg aggatctggt aaccgtggtg ctgggcgagg cggaagtcgg catggccttc
    26881 tccaaactgc cgttcgacca cctgctgttc accggcgcca ccagcatcgg caagcacgtg
    26941 atgcgcgccg ccgccgagaa cctcaccccg gtcaccctgg agctgggcgg caaatcgccg
    27001 gccatcgttt cggcctccgt acccctgagt gacgccgccg aacgcatcgc cttcggcaag
    27061 accatgaacg ccggtcagac ctgcgtggcc ccggattacg tgctggtgcc aaaagaccgc
    27121 gtagagggtt ttgtcgaggc ttaccgcaaa gcggtgcaga gcttctaccc gacgctggcg
    27181 aacaacccgg attacaccgc gatcatcaac gagcgccagc agcagcgcct caacggctat
    27241 ttgcaggacg ccgagagcaa gggtgcgacc atcatcccac tgtacccaga agcccagggc
    27301 cggcgcatgc cgcaggccgt gctgcttaac gtgaccgatg agatgaaggt gatgcaggac
    27361 gagattttcg gcccgctgct gccgatcatc ccctacgaca agctggaaga cgccttcgcc
    27421 tttatcaatg agcgtgaccg accgctggcg ctgtactact tcggctacga caggagcgaa
    27481 cagcagcgcg tgctgcacga gactcattcc ggcggtgtgt gcctcaacga caccctgctg
    27541 cacgtggccc aggacgacat gccattcggc ggcgtcggcc cgtccggcat ggggcattac
    27601 cacggccatg aaggcttcct caccttcagc aaggccaagg gcgtgtttat caaacagcgc
    27661 ttcaatgccg ccaagctgat ctacccaccc tacggcaaag ccctgcagaa gctggtgtac
    27721 aagctgttcg ttcgctaacc caaaatggcc tgaatcggtg ataacaataa atgcacaaca
    27781 ccacacttga agcaccggcc ctaactcgac gcaacctact caagatcggc ctgatgggct
    27841 cggcctttct cgccacggcc gggctggccg ccagcctcag cggctgttcg gcaagtacac
    27901 cgaaagccgg cttcgcggtg atccgcgaat ccgatctggc ttttctgaat gctttgattc
    27961 cggtgatgct ggccggagca gtaacgaccg agaagatgcc tgctgccgtc agcgccaccc
    28021 tcgaaaacct ggactacagc ctgaatcacc tgtcgccgga aatgctcaag ctgaccgtgc
    28081 agctattcga tgtgctcgcc atgccgatca cccgcggccc actaaccggc gtctggggcg
    28141 gctgggaaaa cgcatcgcct gccgacgtac agggatttct cgaccgttgg cagaacagcc
    28201 ccatcggttt gttgaagatg ggccacgcct cgctgctgca actggtgatg atgagctggt
    28261 atagcagagc cgaatcatgg gcccactgcg gctaccccgg gccgccgacg gtctaacgcc
    28321 gcgtcgatcc cgtagggtgc gctgtgcgca ccgcctaagc ctatttttgg tgcgcatggc
    28381 gcaccctacg ggcctaacca gcaaccgcag cgctacccat tacccagccg ccaagccggc
    28441 cagaacaaga aagagagccc gcaatgcccg tacccgatat gttcaagcaa ggcctggcca
    28501 acggctggaa aacctacaac ggctctcgct tggacagcga tctgaccctg gaagccgacg
    28561 tggccatcgt cggcagcggc gcgggcggcg gtactaccgc agaaatcctc agcgcggccg
    28621 gcttcaaggt gctgctgatt gaggaaggtc cgctgaagac tagcgatgat ttcaagatgc
    28681 tcgaagacaa ggcttacgcc agcctctatc aggaaggcat cggccgcatg agcaaggatg
    28741 gcgccatcac tatcatgcaa ggtcgtgcgg tcggcggcac cacgctgatc aactggacct
    28801 cgagctttcg cacgcccgcg ccaaccctgg aacactgggc caaggagcat ggcgtaaaag
    28861 gccacagccc cgaggaaatg gccccctggt tcgagcagat ggaacagcgt ctgggcgtcg
    28921 ccccctgggc cgtgccgccg aatgccaaca acgcggtgat cagcagcggt tgtgaaaagc
    28981 tcggctacga gtggaaggtc atcccccgca acgtgcgcgg ctgctggaac ctcggttact
    29041 gcggcatggg ctgcccgacc aacgccaagc agtcgatgct ggtcaccacc attccggcga
    29101 ccctggataa aggcggcgaa ctgctctatc tggcccgcgc cgacaagctg ctgatcgagg
    29161 gcgaccaggt cacaggcatc gaatgcctcg gtatggatga acgttgcgtc gcgcccaatg
    29221 gccgcaagat cacggtcaag gccaagcatt acgtgctctc aggtggcggc atcaatacac
    29281 cggggattct ccttcgctcg aacgcaccaa acccgcacca gcgcaccggt aaacgcacct
    29341 tcctgcacct ggtgaacttc tctgccgcgc tgttcgatca ggtaatcaat cccttctacg
    29401 gcgcgccaca gtcgatctac tccgaccact tccagtggga tgacggtacc accggacgcc
    29461 tgtcctacaa gctcgaagta ccgccgctgc agcccgcgat gacctccacc ctgctcggcc
    29521 gcttcggtat cgacaacgcc atgcgtatgg agcaactgcc caataccaac gtgatgctgg
    29581 ccctgctgcg cgacggcttt cacccggaca gtgccgaagg ccgggtggag ctgcgcggcg
    29641 atggcagccc ggtactcgac taccagatga ccgactacac ctgggacggc gtgcgccgcg
    29701 ccttccatag catggcggaa atccagttcg ccgccggggc caaggcggtg ctgccgctgc
    29761 acgccgatgc cggctatgtg aaaaccctgg gcgaggccaa gcagctgatt gacacactgc
    29821 cactggagat ctaccgcacc cgcctgggtt gcgctcatgt catgggcggt tgcgccatgg
    29881 gcgaagatcc ccgccaggca gtcaccgaca gcctcggccg ccatcaccaa ctgagcaacc
    29941 tgtcgatcca cgacggctcg ctgttcccca ccagcatcgg cgccaatccg cagctgtcga
    30001 tctacggcct gaccgccaaa ctggcgacaa agctcgccga acgcctcaag cacgcctgat
    30061 gccagggtat tcacgggccg ttctgtgccc gtgaataccc tcgataaccg gcggccgact
    30121 tggccgcagg gaagcgctgc gctaccatcc gactccccaa cggacccgcc aggacgtcac
    30181 gatgaatcga gtgttatacc caggcacctt cgaccccatc accaaggggc acggcgacct
    30241 ggtcgagcgt gccgcgcggc tgttcgacag cgtgatcatt gcggttgccg ccagcccgaa
    30301 gaagaacccg ctgttcagcc tggaacaacg cgtcgagctg gcccgggaag tcacccaaca
    30361 cctgcccaac gttgaagtag tcggcttctc caccctgctc gcgcactttg ccaaagaaca
    30421 aggcgcgaac gtgtttctac gaggcctgcg cgcagtatcg gacttcgaat acgaattcca
    30481 gctggccaat atgaaccgcc agttggcccc cgatgtggaa agcctgttcc tcaccccgtc
    30541 ggaaaagtac tcgtttatct cctcgaccct ggtccgcgaa atcgcagctt taggtggtga
    30601 tatctccaac ttcgtgcacc cggccgtggc caccgcgctg accgaacgtt ttaagcgcta
    30661 agccattacc acatggccgc gtgcatgcgc ggccctgatg cggcacaatt tgcgcattgc
    30721 ttccccaagt tttatggctg tacccaagag ggcacagcag gagtgcgtaa tgtccctgat
    30781 catcaccgac gactgcatca actgcgacgt ctgcgagccc gaatgtccga acgctgcgat
    30841 ctcccagggc gaagaaatct acgagatcga ccccaacctg tgcactgaat gcgtcggcca
    30901 ctatgacgag ccgcagtgcc agcaggtctg cccggtcgat tgcatcccgc tcgatgagaa
    30961 caatgtcgag agcaaggatg aattgatgca gaagtacctg atcattaccg gtaaagcatg
    31021 aaaaccggcc gcctgatccg cggccttctc ccctggcgca ccctgctcgg tgcgcttgct
    31081 ctactctgtg cccttgagct gcatgccgca gcccaacctg aacgcatcgc tattgccagc
    31141 gcacacccag ccgccaccgt ggctggccag gaaaccctgg ccctgggcgg caacgccttt
    31201 gacgcggcag tggccatcag cgcagcgctg gcggtagtcg aaccctatgg ttccggtctg
    31261 ggcggcggcg gcttctttct gctgcgcgaa gccggcgaat caccgaccta ccgctttctc
    31321 gatgcgcgtg agcgtgcgcc acaagcagcc cacgccgacc tgtaccgggt cgagggcgag
    31381 ataaacaaag atctttcact gaatggcgcg ctggccgctg ccattcctgg tctgcccgcc
    31441 gccctagtcg agctggccga acgctttggc cgcctgccgc tgcgcgactc gctggcgccg
    31501 gccattcgcc tggcgcgcag cggcgtcagc atcgaccggg tttaccgtga gcgcgccggc
    31561 tggcggctcg aagccatgcg ccaggacgcg gaaactgcgc gaatcttcct cgacaaaggt
    31621 gaaatccccg aagagttcgg cctgctgcgt cagccggaac tggccagcac cctggagcag
    31681 ctgggcacaa agggccttca gggcttctac ggcggagcag tggcggaaca gctggtcaaa
    31741 gcggtgcgtg atgccggcgg tatctggacc ctcaaggatc tgaccgacta ccgcatcgtt
    31801 gaacgccagc cgctgcgcgt ttcgctggat cagggccgcg agctgattag cgcaccgccg
    31861 ccatcagccg gcggcatcgc tttagggcaa agcctgctga tgctgcagca actgccctgg
    31921 caacaggctg atcgcgtgca acgcactcac ctggtggtgg aaacactacg ccgcgcctac
    31981 cgggatcgcg gcctactggg ggaccccgac tttgtccgca acccagcgca gcaactacta
    32041 gcccctgatt acctgaaaca gctcgccagc agcatcgacc cgcaacgcgc cacgccgagt
    32101 gacagcctgc cacccagcaa aacctggcag gaaggcgacc acactaccca cttctcggta
    32161 ctcgacaccg agggcaatgc ggtggcggcg acgctgtcaa tcaatctgcc attcggcgcg
    32221 gcctttaccg cacccggcac cggggtgctg ctaaacaatg agatggacga ttttgctgcc
    32281 gatgtacaag gcagtaacgc ctatggtctg agcggcagcc aggccaacag catcaccgcc
    32341 ggcaagcggc cgctgtcgag catgagtcca agctttatcg aaagtaccga ggacttcgcc
    32401 agcttcggca ccccaggtgg cagccggatt cccagcatgg tgctgctggc gatgctgcag
    32461 tacctggatc aacaaccgat tgagcgctgg cccagtgtca gccgctacca ccaccagttt
    32521 cgcccggacc tgattgagca tgaaccagat acgttcagcc cagcagaaat cacggcgcta
    32581 gaggcgctcg ggcatcaatt gaaatcaaca ggcaggcagt acggcaatca gcaggtgctg
    32641 tactgggaca agcaaggcgg gaaggttgca gcggcagaag atccgcgcgg gatcggtact
    32701 gcggtggtca caccagccgt accgcagtaa tgcggatcac tgatgcgcca aacaggactt
    32761 ttcagggctg ccaaaccaaa cacagtggca gcccacacgc agagaacgct atcagttctt
    32821 cttgtagccg ctagtcgtgc aacccaggca acgcacaaag gcggcctctc cgggctcaac
    32881 aaccagagct ttaccagcct caccaatatt gccgccagca gccgtgaatg gcaggctcac
    32941 cacgaaagcc acagcgccga tggcagttgc gccaatcagc aagggccggg caaccagcag
    33001 atcgccaatc atcgaaaatg cttttggcgc gttagccgtg taaagagggt cgccactgct
    33061 gttctgctgc gctacgtcag cataagccgg cagcgccagc aggccagtgg tcagcgccag
    33121 agcagcagcg gttgagcgga acagtttcat ggggcgatcc ttcagctatt aggaggcggt
    33181 aaagctaact ataacagtgc attggtaatt gtcagcgtga cggccgtcaa tcgccataaa
    33241 aggcgtattc aggtttttcc ggcataacag ggtcggtaaa acccgaattt ttcgcctcag
    33301 atcctaaaca gtgctcttag cgctggcagc gaggacagta gacgctggca cgctggccca
    33361 gtttgacctc gcgcagagtg ctgccgcagt gcttgcagaa ttccccgccg cgaccgtaga
    33421 caaacagctc ctgctggaaa tagccgggct gaccatcgcc gccaacaaag tcacgcaggg
    33481 tggtgccacc gcgctcgatg gcgtggacga ggatgcgttt gatctcatct gccaacgtca
    33541 gataacgcgc ccgcgacacc gagccggctt cgcgacgagg atcaataccc gcagcaaaca
    33601 gcgcctcgct tgcgtagata ttgcccaccc cgaccaccac ggcgttatcc atgataaagg
    33661 gcttgatcgc catagcgcgg ccacgggaca gctggaacaa tcgctcgcca tcgaacagct
    33721 cggtcaacgg ctccggcccc agcttgctga gcagcacatg gttcaacgga tcactgctcc
    33781 acagcagcgc gccgaagcgc cgcggatcgg tgtagcgcag ggccagaccg gactccagct
    33841 caatatccac atgctcatgt ttggccgccg gtaaacccgc ctcgaccaga cgcaggctgc
    33901 cagacatgcc cagatgactg atcagactgc cggtttcggc cttgatcagc aggtacttgg
    33961 cacgccgttc gacgcactcg atacgctgcc cagacagctg gatatccagg tcctcgggta
    34021 tcggccagcg taggcggcgc tcgcgcacga tcacccggct gacacgctgg ccttcaagga
    34081 atggcgcaat gccacggcga gtggtttcta cttcaggaag ttctggcata acgagtgatc
    34141 agttgagcaa tccgagcggg cgcctacctt agcaggatcg ccccgcagcg acgaggcagc
    34201 ctgttaactt gcccctaagt cgcgaatgct ctcgcgcagg ttctcgaagt catagtcgga
    34261 taggccaaca tactccagca ccagctgctc aatgctttgc cactcatggt cgtccggctg
    34321 attgcccaaa acgcgatggc tggcgcagat gtgctcggcc atcttcagaa tcgccagcag
    34381 ggttttcagc tgcgcatcgc gactggcgtc ctcggtaaaa atcgccaggg cgttgtggtg
    34441 gctggcaatg gcttcgcaca ggtgcaacgg caggttccac gacttggcgg tgaagtagcc
    34501 gaccaccgcg tggttggtgt tgagcaagcg gttttcggta tccaccacgc ggcgctcgcc
    34561 ggtggcggtg gcataggcct cctcgagcac gttcatgtaa ttggggaagc gcttgatcat
    34621 cagcgggatg ccacagttgt ggaacaaccc aagggtgtag gcctcatccg gcgagtggta
    34681 gccgatgcgc ttggccaggg tcagacaggt catcgccaca tcctgggctg tgtcccagaa
    34741 gcggttgagg gtgacgatgg cctcatcggt cagctcgcca cgaatcgact gcgcattgat
    34801 cagattgatc accgtgttgc agcccagcag gttgaccgcc tgctggatcg aggcgatgcg
    34861 attggccaga ccgaaaaacg gcgagttcac cagtttcagc agcgcgccgg ataagcctgg
    34921 gtcctggcta atcagcttgg cgatggcgcg caggtccggg ctcggcatca cctgttccat
    34981 ctgcaaatcc accatgatct gcggctgcgg cggcacgctg atgccctgca gcacttgctg
    35041 aatatattcg gcggaaagct cttgggacat gacgggaaca accggaatga gaagtgagcc
    35101 acagtctagc taaacccccc gcacttccac aggcgacttt tgtaaccgcc gcgacgctac
    35161 tgaacccgct ataattccgc tcttttttcg gagtccccag catgtccctg cccagcctgc
    35221 gcctgaaagc caacgccgat cgacgcctgc gcgccggcca tctgtgggtc tacagcaacg
    35281 aaattgatgt cgccgccacc ccgctcagcg gtttccaggc tggcgaccag gccatcctcg
    35341 aggcgaccgg cggtaagccg ctgggcgtcg tcgcgatgag cccgaacaac ctgatctgcg
    35401 cgcgcctgct gtcgcgtgat gtcaaacacg tgctggacaa gtccctgctg gtgcaccgca
    35461 tcaacgtcgc cctgtcgctg cgcgagcggc tgttcgacaa gccctgctac cgtctggtgt
    35521 atggcgattc agacctgctg ccaggcctgg tggtcgaccg tttcttcgac attctggtgg
    35581 tgcagctggc ctccgccacc atggaacgtc acaaggatga cgtgctcgcc gcgctgatcc
    35641 aggtctgcaa gcccagcgga atcctgctca aaaatgattc cgcagcccgc gacgccgaag
    35701 gcctggcgcg ttatgtggaa accccgtttg gcgtggtgcc agagtgggtc gccctggaag
    35761 agaacggcgt gaaattcgaa gcgccagtga tcgaagggca aaaaaccggc tggttctatg
    35821 accaccgcat gaaccgcgcg cgcctggcgc cctacgtcaa aggcaagcgc gtgctcgacc
    35881 tgttcagcta catcggcggc tggggcgtgc aggcggcggc gttcggtgca agcgacgtga
    35941 tgtgcgtgga cgcctccggt ttcgccctcg atggcgtgga gcgcaacgcc gcgctgaacg
    36001 gtttgaccga tcaggtcgcc tgcgtagaag gcgatgtgtt cgaagcgctg aaacagctga
    36061 aagcctcgga agagcgcttc gacgtgatcg tcgccgaccc acccgccttt atcaaacgca
    36121 agaaagacca gaagaacggt gaaggcgcct atcgccgcct taatgaacag gctatgcgcc
    36181 tgctcaacaa ggacggcatc ctgatcagcg ccagctgctc catgcacctg gaagaagaca
    36241 acctgcagaa catactgctg accagcgccc gccatctgga ccgcaatatc cagctgctcg
    36301 agcgcggcgc ccaaggcccg gatcatccgg tgcacccggc catcaatgag acccgctata
    36361 tcaagagcct gaccgtgcgt ctgctgccca acagttgagc acctgcacct gccgagtgcg
    36421 tgtcggcagg tgcttctttc tctcccgcgc tgttaccact ccccacaccc gcctggcgca
    36481 accccgcgcc tggactaggc ttagacccca gcaccgctgc gctcgcgatg tgccgccgct
    36541 gactggcaca gtgcttgcgt aacttgaaca ttcaaacaag tacccgttgc agcataaaag
    36601 cgggcgcacg aattcaagca ttcagataac ggccacaagc tgctgtctgt tcagagcaaa
    36661 atcggggagg ggtatggctg acctggctgg ggacaaaccg ttcctgcaat tcaccaacgt
    36721 taagaagact tacgaccaca agaatctggt ggtcaaggat ttcaacctgg atgtggccaa
    36781 gggcgaattt atcaccctgc tcggcccttc cggctcgggt aaaaccactt gcctgatgat
    36841 gctcgcaggg ttcgaggacg tgaccagcgg cgagatcctg atcaacggcc gctcggtcac
    36901 cagcatcgca ccctaccagc gcaatatcgg catggtattc cagcagtacg cactgttccc
    36961 gcacatgacc ctgcgcgaaa acctcgccta cccgctaaag atccgcaagc agcccaaaga
    37021 ggtgattcgc gccaaggtcg atgagtacct gtccttggtc gaactgctgg atttcggcaa
    37081 ccgcttcccc gggcaactct ccggcggcca gcgccagcgt gtggccctgg cccgcgcgct
    37141 gatcttcgcc cccgacatcg tattgatgga cgaaccgctg ggcgcactgg acaagaagct
    37201 gcgcgagcag atgcagttcg agatcaagcg cctgcacgaa cagctcggct tcactgtgat
    37261 ctacgtgacc cacgatcaga ccgaagcgct gaccatgagc gaccgcatcg ccgtgttcaa
    37321 caacggcatc gtccagcagt gcgcaccgcc ccatgtgctc tacgagcgcc cggccaacct
    37381 gttcgtcgcc gattttatcg gcgagagcaa caagctcaag ggcgtgatcg aatcggtcgg
    37441 ttctgagacc gtcgaagtgc gcctggacga cggcgccatg gtcagttcgc tgaaggccaa
    37501 ttgcacggaa gtcggcttgc ccaccacggt gtcgatacgc ccggaaaaac tcacctttac
    37561 cgaccgcctg ggtgccgccg gcaaccaggt catggcgcgt ttcgtcaccc gccactatgt
    37621 aggcgaatac atccgctacc acttcgaaac cgccgacggc accgagctgg tggtgaagaa
    37681 catgaatgac agcagcgccc cgcaactcag tgccaaggaa gaagtagccc tggcctggct
    37741 gccgcaagac agccaggccc tggatcgccc aatcgccaca ccaacctgac ccccataact
    37801 agaagcagaa gcaatggagc acagcaatgg ctagaacact gaccacccgc ttttccacct
    37861 tcgccctcgc cgccgccctg ggcacggcaa gtttcgccgc cagcgcgcag gagaccctga
    37921 cagtggtgtc atggggcggt gcctacggcg cggcccagca gaaacacgtt atcgacccgt
    37981 ttatgaaaga caccggcaac aaggtgctgt tcgaggatta caccggcggt gtggccgaga
    38041 tcaaagccca ggtcgagtcc ggcaatatcc agtgggatgt ggtcgacttc gaggtaatcg
    38101 accttgagcg cgcctgttcc gaaggcctgc tggaaagcat cccgcgtgac atcctgccgg
    38161 ccggtatcga cggcgtgccg gctgagaagg actttatccc cgaagccctg gccagtgagt
    38221 gcgcggtcgg caacatcgtc tggtcggtag tattcgccta caacgagaaa accatcggcg
    38281 gcaccaaggc tgcgagcatc accgatttct tcgacaccag caaaatcgct ggcaagcgcg
    38341 ccctgcgcaa acgcccgcag gtgaacatgg aatgggcgct gctggctgat ggcgtgccac
    38401 cgggcgaagt ctacgacgtg ctgtccaccc cggaaggcca ggctcgcgcc ttcgccaagc
    38461 tggacaccat caagaaggac atcgtctggt tcgactcctg gtcccaggca ccacagctgc
    38521 tcaacgatgg cggcgcggtg ctggtgcaat cggccaacgg ccgcttctat gacgccatcc
    38581 gccaggaaaa caaacccttc gttatcgtct gggacggcca cgtctacgac ctcgacgctt
    38641 gggccatcgt caaaggctcg ccgaaaaagg acctggcatt gaaattcatt gcctacgcca
    38701 ccggttccac cccactggcc ggcatgccgg acgtggccta cggcccaacc cgcaaatcct
    38761 ccatgccgct ggccgaccag agtgccgcac cgcacttgcc gaccgctcac ctgagcaaag
    38821 gcatccaggc cggttcggac ttctgggctg actatggcga atccctcggt gagaaattca
    38881 acgagtggct gttgaagtaa cccgctatga cccctctccc gccggagagg ggtcaggatt
    38941 ttcggagtaa cgccttgtcc tcccttacgt ctaccgaagc cagccaagcc tccagcgccc
    39001 gcaacaagaa gcgcctcacc gcctttctgt ttgttgtgcc gttgctggtg tttatcctgc
    39061 tgaccttcgt cgcaccgatt gccagcatgc tctggcgcag cgtccaccac ccgacggtgg
    39121 ccgccctgat tccgctgacc ctgagcgagc tgcaacgctg ggacggcaaa gcgattcctg
    39181 atgagaaaac cctgagcgtg ttcgctcagg agctgcatag cctggccaaa gagcgccagt
    39241 ccggcaaatt ggccgaagag ttcaaccgcg cctcggcggg catgtccagc gtggtcaaat
    39301 ccactgcgcg caagctcagc cgactgcaag atgcgcaact gcagcaagag ggtgccgaca
    39361 tcctgctcaa gtcgcaccgt aactggagca agccggaaat ctggtacgcc atccagcgcg
    39421 cgggcaaacc ctacacctat gactactacc tgaccgcgct cgacctggag ctgcaccagg
    39481 aacagggcat tcaggtgcgc gaagacacgc agatctacct gcagctgtac agcaagaccc
    39541 tgaccatggc cctggttatc accatcttct gcgccctgct cggctacccg ctggcctact
    39601 acctggccgg cctgccggag aaccgcgcca acctgttatt ggtactggtg ctgctgccgt
    39661 tctggacttc actgctggtg cgcaccacgg cctggatcgc cctgctgcaa accaatggag
    39721 tgatcaactc cttcctgctc ggtatcggcg taatcggcca gcccatcgag atgctctaca
    39781 cctcgtttgc cacggtagtg gcgatgaccc atatcctgct gccgtttatg attctgccgc
    39841 tgtacagcgt gatgcgcggc atcgacccca gttacctgcg cgcggcgctg tcgctgggcg
    39901 cgcggccgat cccggcattc ctgcgcatct acttcccgat gaccctgccg ggcctcagcg
    39961 ccggcgcgct actggtattc atcatctcgg ttggctacta catcaccccg gcgctggtcg
    40021 gcggcaccga tgggcagatg atcagcaaca tcatcgcctt ccatatgcag cgttcgaaca
    40081 actgggaact ggccgccgcg ctgggctccc tgctgctcgg cctgatcctg ctgctgtact
    40141 ggctgtacga ccgttttgtc ggcgccagca atatcaaact gggctaagga acagacgatg
    40201 aagattcccg cctactacgg cttctggcac cacctcggcg cttggctgct gaaaaccagc
    40261 tcctggctgg tgctgctgtt tctgatcctg ccgatactgg tgatcatccc actgtcgttc
    40321 aacgtcgagc cgttcttcag ttttaccgaa ggcatgctca ccttgcagcc cgaggcctac
    40381 tcgctgcgct ggtacagcgc cattttcagc gacgacaagt ggctgctggc gatcaagaac
    40441 agctttctga tcggcatctt cgccaccctg atcgccaccg tgctcggcac ctgcgcggcg
    40501 gtgggcctgg cccgcgacga tatgccgatg cgccggctga ttactgccct gctgctgtcg
    40561 ccgatgattg tgccgctgat catcaccgcc gccgggatgt tcttcttcta ttcggatctc
    40621 ggcctggccg gcaactacct cggcgtgatc atcgcccacg ccgccctcgg cacaccgttc
    40681 gtgatcatca ccgtgaccgc caccctcacc ggcttcgact acagcctggc gcgcgccgcg
    40741 ttgaacctgg gcgccacgcc gctgcgggtg ttcttcgaca tcatcatgcc gctgatccgc
    40801 cccggggtga tttccggcgc gctattcgct tttatcacct cgttcgacga ggtggtggtg
    40861 atcctgttta tggccggccc acaacaacgc accatccccc ggcagatgtt ctcgggcctg
    40921 cgcgagcaga tcaacccaag catcctggcc atcgccaccc tgctgatcct agtgtccatc
    40981 gccctgctgg tgaccatcga actgctgcgc cgccgcgccg agcgcatgcg cggtatcgaa
    41041 ctgcccacct gaaagcccgc cccgaaaggc gggcactgcg acctactgga taccgcgtcg
    41101 gttgaccgac cactgcagac tgctgcaaag gctgcagcgc gcaccgaaga aggtgccacg
    41161 cgcgctctga tcgtccatca gctggaagcg gaaccaggcc gtggttggcc cgcggtaggc
    41221 accaccgtca cccacgggtt caaagtggct gacgaagcgc cgctcgcccc aaaacaccgg
    41281 cacattggta cgcagataga ccggctgagc gttcaggtaa ggaaatgcga tggtgtctcc
    41341 gccgccggac atcaggaaca ttggcccctg ctggcgacgc tgcgaggcgg tatcgtggcc
    41401 caggccgatg gtgtaaggct gaatcggcgc agtggttcgc acccgctcgt cctgcccagc
    41461 catgatcgac ccgccgccgc cctgagagtg ccctgaagta gccacgcgcc cagtattgag
    41521 cttgccagca taggtgccat aagcgttgct gttttcctgc accaggtaat ccaggcaggc
    41581 cagcatgtcc tggccggtgc cagcgttgga ggtttccgct gccgccacca caaagccgtg
    41641 actggcccag tgcgacagca acccggcata ggttgacggc ccagtaccgg tgccattgcc
    41701 ccagagaatc actggatggc gcacaccgcc ctgccccagg ttgctggggc ggtaaatgcg
    41761 acagctcggc ccctcgctct ggttgctggt ggcatagggg ccgctgcgct cgaagctgga
    41821 gacattcggg aagggtgccc ccggcgtgtc cggtagcggt gcagccaaag cgctgatgga
    41881 aataaacagg ctggcggcag ccaggccgcc gggtttcaag tcgagcatcg aaggttcctc
    41941 gttctgttgt ctttttatac gagtcgccat cgccctgccg acccaccaca tcggactggg
    42001 cggcgcggca cacaactctg cgctggcgat tatgggtgcc agccgcatcg gcaatatgca
    42061 ccgccaagca ccctaaatga ccaatcagtc aagccggcag tcgcccaaag gaggtaagcg
    42121 gatgaacggc atcgcaccaa ggagcgacgc taatcgccgc ctgccgcatc caccgcaagc
    42181 gccgacaatc aatgcgcctt ggctcagcgg cagcggcggg cgtagaatcg gcgcatctct
    42241 cgccgtccat cccctggcgg gtctctgagc acgagtcaga cagcggccac cccgcgtcgt
    42301 ccagtggtcc ccgccgaacg cacggccatc tcccgtttgc atccggtgca gtcgacgctc
    42361 accatacgaa catcacctga ttaaccgcag gaactgtgtc atgcccgatt accgctcaaa
    42421 aacctccacc cacggccgca atatggctgg cgctcgcgcc ctgtggcgcg ccaccgggat
    42481 gaaggacgaa gacttcaaga agccgatcat cgccatcgcc aactccttca cccagttcgt
    42541 gcccggccac gtacacctga aggatatggg ccagctggtc gcccgcgaga tcgaaaaagc
    42601 cggcggcgtg gccaaagaat tcaacaccat cgcggtcgac gatggcatcg ccatgggtca
    42661 cgacggcatg ctctattcgc tgccgagccg cgagatcatc gccgactccg tggagtacat
    42721 ggtcaacgca cactgcgccg acgccatcgt ctgcatcagt aactgcgaca agatcacccc
    42781 cggcatgctg ctggcatcgc tgcgcctgaa tatcccggtg attttcgttt ccggcgggcc
    42841 gatggaagca ggcaagacca agctggccag ccacggcctg gacctggtcg atgccatggt
    42901 gatcgccgct gacgattcgg ccagtgacga aaaagtcgcc gaatatgagc gcagcgcctg
    42961 cccgacctgc ggcagctgct ccggcatgtt caccgccaac tcgatgaact gcctgatgga
    43021 agccctgggc ctggcgctgc ccggcaacgg ttcgaccctg gccacccata gcgaccgcga
    43081 gcagctgttc ctacaagccg ggcgcactgc tgtcgagttg tgcaaacgct actacggtga
    43141 aggcgactcg tcggtgctgc cgcgcaatat cgccaacttc aaggcgttcg agaacgccat
    43201 gaccttggac atcgccatgg gtggttcaac caacaccatc ctgcacctgc tggcagcggc
    43261 gcaggaaggc gaagtcgatt tcgacctgcg cgatatcgac cgcctgtcgc gcaaagtgcc
    43321 gcagctgtgc aaagtcgcgc cgaacatcca gaagtaccat atggaagacg tgcaccgcgc
    43381 tggcggcatc ttctccatac tcggttccct ggcccgcggc ggcctgctgc acaccgacct
    43441 gccgaccgta cacagtaagt ccatggccga agccatcgcc aaatgggaca tcacccagac
    43501 cgacgatgaa gccgtgcaca ccttcttccg cgcaggcccg gccggcatcc cgacccagac
    43561 cgcattcagc cagagcaccc gctgggacac cgtggatgac gaccgcgaga acggctgtat
    43621 tcgcagcgtc gagcatgcgt actcgcaaga aggcggcctg gccgtgctgt acggcaacat
    43681 cgccgtcgac ggctgcgtgg tgaaaaccgc cggtgtggat gagtccatcc acgtcttcga
    43741 aggcaacgca aaaatcttcg aaagccagga cagcgccgtg cgcggcatcc tcgccgacga
    43801 agtgcaggcc ggcgatatcg tcatcatccg ctacgaaggt ccgaaaggcg gcccgggtat
    43861 gcaggaaatg ctttacccaa cctcttacct gaaatccaaa ggcctgggca aagcctgcgc
    43921 cctgctcacc gacggccgct tctccggcgg cacctcgggc ctgtccatcg gccacgcctc
    43981 gccggaagcc gcagctggcg gtgctattgg cctggtgcgc gaaggcgaca agatcctgat
    44041 caacatccac gagcgctcga tcaacctgct gatcagcgat gaagagctgg ccgcacgccg
    44101 cgtggaacag gacaagaaag gctggaagcc ggtggaagta cgtccgcgca aagtcaccac
    44161 agcgctcaag gcctacgccc tgctcgccac cagcgccgac aagggtgcag tgcgcgacaa
    44221 ggcactgctg gacaaactgg tgccttaagc caataggcgc actcagaagc ccggcctagt
    44281 gccgggcttt tgctttcggc acaacaggtg cgcgctgtat cctgccaggc atgcaggttt
    44341 caaggactag ggacaatgct ggtagccagg cgagtgcaaa aaaccgccga gaaacacgac
    44401 gaagagagca ggttagaaga actgccgctc agcgactttc gtcacacgcc tgcttgggtg
    44461 ctgctcggtg agcccggcgc aggcaagagc gaagccttga ggcaagaagc tctggaagtc
    44521 ggcggcgagt acgttacggt gagcgagttt gtagaaacaa cccctccgct ggcggagtgg
    44581 tacggcaaga cgctgtttat cgacgcgctg gatgaagccc gggcctcagg atcagtaagc
    44641 ctgattctgc atatccggca gcagctacgt cagcttggca aaccagcttt ccgcctttct
    44701 tgtcgcgctg cagactggca tggctcgacc gacaagactg agctgcagag catcagcccg
    44761 gacaaccaac tgaccgtgtt acagctatgc ccgctgagtg atgacgacat ccagcaaatc
    44821 ctgcaaaacc aattcaacat tgccgagccc aaaaccttca tcgaacaggc tgagcagcat
    44881 gggatagccc agctactcag caacccgcaa atgctgggac tgatggccag agcggtcagc
    44941 ggtgggtcct ggccggaaag tcgcagcgag gtttatcgac tagcctgcga aaagctggca
    45001 caagagagca acgctctgca ccggcaaatt agcaaggcca aagcagtcga tggcgaaaca
    45061 ttattgaagg cggcggggca gctatttgcc atacagctgc tgtctggcaa ggcaggcatt
    45121 gccaccgacc tgaacgccag caataccggg aatccagagc tagatcagtt caagccagaa
    45181 agctccgctg cagctagaaa agcactgcaa tctgcgttgt tcactcaagc tcccggtcag
    45241 gctgaacgct tggctccctg ccaccgcagt attgccgagt atctggccgc aaaatggctc
    45301 gcaaacaaac tggatagaga aagcctgcca caggggcgac tgcttaatct gctgctcggc
    45361 tttgacggcg gcgtggtagc cgacttacgc ggcctgctgg cgtggctggc tcaccacagc
    45421 acatcaatac gttcgcgact gataaaggtc gaccctctcg gtgtggtgct atacggcgat
    45481 gttcgcccat tcagcccgga aaacaagcgc catctactgc atgccctacg gcaacaggca
    45541 gagcggtttc ccggatttcg ctggagcctc tggcaggaca tgacaggatt ccgcgcactg
    45601 gccgacgagg ccttgctcga agacttcctg cgtacccttc aagcacctga gcgtgacaag
    45661 gctagccagt cccacatgga ctgtgtgttg gatattctcg aatacagcca tacctatgcc
    45721 tgcatggcgg ccccactgct tgcagtcatc aaagatgcat cgctttggcc acggcttcgg
    45781 tatagcgcac tcaggatctg gttcaagctg gccactccta tagaaagcaa agtcttgttg
    45841 tcacagattt gcagtgacca ggttgaggac gaagatgacg aactgctgag tcggctactg
    45901 gatcggctat atccggagtt tttgattccg gacgagctac tccaatgcat tcactccccc
    45961 aagaacacac atctgatcgg cggctatcgg atgttcttag agcacggatt atcaaaacta
    46021 gctccggcaa atcagttgcc cgctttgtta gatggcctct gtgcaaaacg cgattttttt
    46081 gatgaagaca aaaccgagcg gatggtttcc cgttcggtcg accggttgct tgtccgtacc
    46141 ctcaatgagc ttggtgattt cgccacagac gagcagcttt taaactggct gggagttggt
    46201 actaataagc atggttactt caaccgggat catgattctc gagcacaaat ccagcgctgg
    46261 ctcgaacagc gtcctgagaa atacaaagcc gtgcttgcgc aatgcgtcga agccaatgca
    46321 cgcggcagca aacatgcctc ctacctcctt ttgcatgatg ccgccccacc agcagacata
    46381 ggcctttggc atctccatct ggccggcata ccagaggaag cgacagcaag aatccatgct
    46441 cagtacgcaa ctcacgccct aatgcatcag cgtggtgccg agggtctatc tctagaggcg
    46501 cttgaagcgt gggcacggga ctttccagaa cgggcagact ggttaaacga aggacttgtt
    46561 tgtgatctgg agcgagagag ctggcgcctg gaagacgcag cacgtactcg cccctacaga
    46621 gaacggcaag ccgaggagcg ccgccaacga actcaagaga ttactcccga gctacccgcc
    46681 attgctgccg gaacagccgc ggcagagcta cttgaagagt tagctttagt ttggttgaac
    46741 gggctccatg atatttatgg ggacacaatt agcgagcgtt ttgccagtta cagcgatctg
    46801 ggtgatgtcc tcatgcaggc agccgagtca ggctttcgga actgcttgca gcgccctgcc
    46861 ctgccctcgg tcgcagaagt catcgatatg gatcaagccg gaaagcgtga ttacatcagc
    46921 ctgccgtacc tgcttggcct gcaactaagc tgggcagatg cacccgagca agtcacggca
    46981 ctgccagatg atcaggttga gttactgctg gctatctatc tctgcacacc gctttttgga
    47041 ctagatagtc tgagcgcctg gtgcgcgcaa actgcacagc aacgccccga attgtttgcc
    47101 agagtactga ctcgccaagc tactaatgct tttgccgcgg gcaatacttc tgcaggtatg
    47161 gcgtattcac tggcaagaga tgcttcctat acgaaggtcg ctcagctgac cagcccagaa
    47221 ttgctgcgac aattccctga aagcgccacc gagcaacagc tccacgacct tcggcagcta
    47281 ctgagagcag ctctagtgac agcacctgaa tgccttgctg cactggctct aaacaagctg
    47341 aaatcgccac aactcaatac tgaacagcgc cttctgtggc tggctctaca gctactgacg
    47401 ggaaaccccg aagacgagag tgagttttgg cagcgccttg gcgaagcaga ggaagccaat
    47461 gtagccatgc aactagcggt tgaggtcttg agacaaggca ttcagctgca gccttgccaa
    47521 ccggaacgga ttgtggggcg cttgatagag acgcttatcc ctcatgccga actggagcac
    47581 cgtaaaggag gagggtttgt tactaccgct atggatcttg gtgacgaact gcgcagcatg
    47641 atcgactggc ttggtgcaca gcctacagta gaagccaata acgagctgca gcacctgctc
    47701 gcttgcccag cgctagcaac tcaggtctac aggattcaga gtacattgga gcagcagcag
    47761 gcgcgcaggc gagaggctga gttcaagttc cttgacctca aggcagtggc agatgtgctg
    47821 gccaatcagg ctccagcgaa tgtggcggat ctgacttatt tgacactgag tcacctagac
    47881 gatatcgccc atgagcttcg caatgccaat gacgacggct atcggatgtt ctggaacatc
    47941 acaaaggctc agccgactgg gcggcgagaa gaaaacctgt gccgcgatgt tctgctgacc
    48001 cgcctacgct cgcgcttgac agcacacggc atcagcatcg ctcctgagta tgaccatgcg
    48061 ggagacaagc gcgccgatct gtatctggac taccgcaatc agttggccct accaatcgag
    48121 gtcaagcgcg atgaccatgc aaaactctgg accgcactgt gcgaccaact gatcacgcaa
    48181 tatgtagatg ccccgaagtc ggaaggtcac ggcatctacc tagtgctctg gtttggcgaa
    48241 agcaagaacc tgaaaagcct cccgaaccga cagccaaaac caacaacgcc tgccgagctg
    48301 aaaaccaagc tcgaagctca actgacaagt gcggagcagc aaaaggtgtt tgtgcgcgtg
    48361 ttggatgtga gctggccgac ctgacgccct ttcaactgac tcccatataa cgcccggcgc
    48421 ggtggttgat cgccagcacc atattcagcg ccagcgcgcc gaggatcgac agcgccacct
    48481 tgtccagcgg cacgaccatt accgccccca tcacgatcat cacatcaatg cccatctgca
    48541 ccttgccggc gcgcaggccg aagcgctcct gcaggtacag cgcgaggata ttcaccccgc
    48601 cgaggctcgc gcggtgacgg aacagcatca acaaccccag tcccatggcg aaaccaccga
    48661 acagcgcggc atacaccacg ttcaggtggg tgaagccaat ccactgcggg gttagttcag
    48721 ccagcagcga gaccatcccc actgcgcaga aggtgcgcag agtgaacggc cagcccatgc
    48781 gccagatggc cagggcgtag aacggcaggt tgatcagaaa gaacaccagg ccgaacggcc
    48841 agccgagcag gtatttcatc agaaaggcca aacccacggt gccgcccgtc agcaaaccgg
    48901 cctgaccgta gagggtgatc cccaacgcca ccatggccgt gcccagcagc agcgccaggc
    48961 catcttccca aaggggatgg cggataaaga tcgcggcaat acgatccggc tcggcgtgat
    49021 tggcatcagc gtcatgcatg gggaacaacc tgtagagggc aaatgacaat agacagcgta
    49081 gcggccagca aggcatcgcc tggcatgggt taacgctacg caacaacgac gcgcagggtg
    49141 gcacgccata ccggcgccca ccctggcgga gatcaatgca gcacggaaaa actcaacccg
    49201 aacgggctat ttgcgatccc acacttcaaa cacatagccg ggcgtttcca gacctgctgg
    49261 gtgctcgatg gccgaggaca ggtgccagtc ggcttcgaca aacgcgggaa agtgcgcatc
    49321 accctgaggg ctgatgccga cgcgggtaag atacaggcgt tcggcctggg ccaggcccaa
    49381 ttcgtacaac tgcgcgccgc cgatcaacat cagctcctcg gcgtcctctt cacgggccca
    49441 ggcatcggca cgttcgagcg ccgcttccag actggcgaac acttccgcgc cctccagctg
    49501 caagccgggc tgacgactga ccaccagatt aagccggccc ggcaagggac gaccgaggga
    49561 atcccaggtc ttgcgaccca tgatgatcgg cttgcccagg gtcagcgcct tgaagtgctt
    49621 gagatccgcc ggcagatgcc agggcagttg gttatcgcgg ccgatcacgc ggttttccgc
    49681 gagggcggcg atcaggctta gaggaagagt cttgttcatg gcggcgagca taacagaggc
    49741 tcacggcacc tcgcagccca cctttggcgc tgccaacctg cgcacgggtt cgagcattgt
    49801 catcctgcgg ttgccaggct gtcgtcgaac cgtcaccgtt ggccaggcag gcttgcactt
    49861 ccaccccgcg caaggagtcg ccgccatgtt cctcaatctc cgcaccctgt tctgcgccag
    49921 cttgctggcc ccgctgctgg ccatgaccag cccggctcaa gccgacagcg tacaagccgc
    49981 catcgactgg gccaggcagc agccgcaaac ccaccacggc aagcccggag gcgacgccaa
    50041 gccgctgatc atcgcccacc gtggcgccag cggttatgtg cctgaacaca ccctggctgc
    50101 ctatgccctg gcggtcctgc agggcgccga ctatatcgag ccggacctgg taacgagcaa
    50161 ggacggccag ctggtcgccc gccacgacaa cgaactgggg ctgaccaccg atgtcgccca
    50221 gcgtcccgag tttgccaacc gccaacgcac ccagacgatt gatggcgtga ccctcaacgg
    50281 ctggttcagc gaagacttta ccctcgccga actgaagacc ctgcgcgcca ttgagcgcat
    50341 cccgcagatt cgcccaggca acgcccgcct ggacggcaac ttcagcatcc ccaccctgca
    50401 ggaaatcatc gacctggtga aaagcctgca actgagccag cagcgggtaa tcggcctata
    50461 tccggaaacc aagcacccga cccacttcca gcaactcggc ctggccctgg agaaaccgct
    50521 ggtcaggctg ctgaagaaaa acggctacca gaacgccaag gcgccggtgt atatccagtc
    50581 cttcgaggtg gaaaacctca agaccctcag ccgcctgacc tccatccgcc tggtgcagct
    50641 gttcggcgcc ggccagccgt ttgaccagca agtacttggc actggcctga cctacgcgca
    50701 gatggccagc ccggccggcc tcaaagccat ctcgcgttac gccgcaggtg ttggcccgga
    50761 gaaaagctac atcatccccc gtgacgccaa cggcaacctt gatgcagcca atgccaccgc
    50821 gttcgtggcc aatgcccatg cccacggcct gaaggtgcac ccctacacct tccgcgcgga
    50881 gaacaccttt ctcccggcca atctgcgcat tggtagcgac ccgcaggcac gcggtgacag
    50941 cgcagcagaa attcagctgt ttctcgatgc cggcatcgac ggcctgttta tcgaccagcc
    51001 ggacatcgct gtgcgcctgc gcgcaaaacc ctgaagcaga gcggatttag ccctacacgg
    51061 cggcagcggt tatgctcagg acctgtcctg atcaatgagt cgccgtgtga gcccactcga
    51121 taaatcccca cccagccgcc tgcaccgcct ctggctaagc gagaccattc gcctgcgcga
    51181 ggaacatgcc ggcccgctgg aagacagcga agccaaccgc cgcgcccagg cccagggtgg
    51241 cgcactcgac gaacgcatcc agacccgcgc ctggttgctg ggcgaacgtg acggccaggt
    51301 gcaggcgctg cagcactggc tacagggcgc gcgcctggcc ggactgtttc tagtgctgct
    51361 ggccattctt agcggcagcg gcttggcggt ggcggcattg ggcgacggcc tgcgcccggt
    51421 caatgtgttc tgggccctgg gcagcctgct cgggctgaat ctgctcctgc tgctgggctg
    51481 ggtactgggc ctgctgctaa gtcgtgacag cgcgggggcg ctcggccatc tgtggttatg
    51541 gttaagcgcc aaactcgccc gcgatgccca ggccgcgcaa ctggcgcccg ccctgctgtt
    51601 gatgttgcaa cgccaacgcc tcagtcgttg ggggctcggc gtgctggtgc agggcttttg
    51661 gctgctgacc ttgcttaccg cgctgctgac tctactggcc ctgctgacaa cccgccgtta
    51721 cggctttgtc tgggaaagca ccctgctcgg cgccgatgcc ttcgtcgcca tcaccaatgc
    51781 cctgagcgtg ctgccacggg cgttgggctt cagcgcgcca acggtggcca tgatccacgc
    51841 cagcaccagt gatgtgtaca acactgagct ggtgcgccag agctgggccg cctggctgat
    51901 tggcgcagtc ctgatctacg gcgtactgcc gcgcctgggc ctgctgctgc tctgcctatg
    51961 gcgctggcgc cggggctgcg cggcgctgag cctggacctc agcctgcccg ggtacagctt
    52021 gctgcgcgaa cgcctgcaac cgagcagcga acgcctcggc gtatgcgatg ccgcgccggc
    52081 gcagctgcat cagccgcaag ccggcagcct tgccgaaggc agccagggcg cactgttggt
    52141 ggcggtcgag ctggacccac agcgcagctg gccgccagcc ttgcccaaga gcatcggcga
    52201 tgccggggtg atcgacagcc gcgaacagcg caatcaactg ctcgaccagc tcaaccgctt
    52261 cccgccggca cgcctgctga ttgcctgcga tccgcgccgc tcgccggatc gtggcagcct
    52321 ggcgctgctc ggcgaactgg cgtgcacggc cagcgccacg cgcatctggc tgctgccacc
    52381 accgcctggc gaagcgctcg acagcgcgcg gttgggcgac tggcaccgtg cactggagca
    52441 actgcaactg agctacagcg acagcgcgcc gttcagttgg ctggagcatg gccatgacta
    52501 aacccgtagc ccggatgcaa tccggggaat atcgacaacg cgaagcaagc tcccggattt
    52561 catccgggct acgagaggca atgtgagcca tccactgaaa ctcgccgtgg tcggccatac
    52621 caatgtcggc aagacctcgc tgctgcgcac cctgacccgc gatgtcggtt tcggcgaggt
    52681 gtcccatcgc cccagcacca ctcgccatgt cgaaggtgcg cgcctgtcgg tggatggcca
    52741 ggcgctgctg gagttgtacg acacccccgg cctggaagac gccatcgccc tgctcgatta
    52801 tctggaacgc ctggatcgcc ccggcgaacg cctggatggc cccgcacggc tggcgcgctt
    52861 tctcgacggc agcgaagcgc gccagcgttt tgagcaggaa gccaaggtac tgcgccagct
    52921 gcaggcatcg gacgccggcc tctatgtgat cgatgcacgc gagccggtac tggcgaaata
    52981 ccgcgacgag ctggccgtgc tgagcatgtg cggccggccc ctgctgccgg tgctgaactt
    53041 cgtcagcagc agccagcacc gcgagcagga ctggcgcgat gccctggccc gccttggcct
    53101 gcatgcgctg gtatgtttcg acagcgtggc gccaccactg gatggcgaac gccgcctgta
    53161 cgacagcctg gcgctgctgc aggaaaaggc tcgcccgcaa ctgcaaaggt tgatcgacga
    53221 tcacctgacg cagcgccaat tacgcaaaag cagcgctgcg cggctgatcg ccgaactgct
    53281 gctggactgc gccgcctgcc gccgtagcgt ggcggcgcaa gcggtgctgg aacagggcgc
    53341 catccgcgac ttgcatcaga ccattcgcca acgcgagcaa cgctgcgtgg aagccctgct
    53401 gcgcctgtat gccttccgtc aggaagacgc caaggccggc gacctgccct tgctcgacgg
    53461 gcgctggggc gacgatctgt tcaacccgga aaccctcaag cagctcggca tcaatatcgg
    53521 cggcggcatg gccgctggcg cggcgaccgg cgtgggcgtc gatctgctgg tcggcggcat
    53581 aaccctcggc gcggcggcgc ttatcggcgc cgtgctcggc ggcagcgcgc aaaccctgcg
    53641 cggctatggc gcgcggctga aaggcaaact caaaggccag cgcgagctga ccgtggacga
    53701 cgccgtgctg cgcctgctcg ccctgcgtca gcagctgcta ctgcaagccc tggaagcccg
    53761 cggccatgcc gccgtcgagg ccatcagcct gagcacgccg caggacgcca gctggcgtga
    53821 aggcaaactg tcggacgcgc tgcacaaggc ccgcgcccac cccgaatggt catccctcaa
    53881 ccccggcgcg cagctggaag acggcgagcg ccaggagcag gtgcagcagt tggcggcgga
    53941 gctgctctag cgttaagaca tcgcggccat ggccgcgatt agcgatcaga ccgcgacggg
    54001 cgcgctgata tgcggatggg cctggtagcc aaccagctca aagtcctcga acttgaacgc
    54061 cagcaggtcc ttaacttcag ggttgagctt cattaccggc agcggcaacg gctcgcgact
    54121 caactgcagg tcggtctgct cgatatggtt ggcgtacagg tgacagtcgc ctccggtcca
    54181 gacgaagtcc cccggttgca agccgcaaac ctgggcgacc atcaatgtca gcagtgcata
    54241 gctggcgatg ttgaacggaa caccgaggaa gatatctgcc gagcgctggt acagctggca
    54301 gctcagcttg ccgtcggcca cgtagaactg gaacagcgcg tggcacggcg gcagagccat
    54361 ctgctcaacc agcgccgggt tccaggcgga gacgatcagg cggcgcgagt ccgggttctt
    54421 tttgatcatc tcgataagct tgctgatctg gtcgatcgac tcaccgttcg gcgccggcca
    54481 gctacgccac tgatagccat agaccgggcc gaggtcgccg ttttcgtcgg cccactcgtc
    54541 ccagatgcgc acaccgttgt ccttcaggta cttgatgttg gtgtcgccct ggaggaacca
    54601 cagcagctcg tggatgatcg atttcaggtg gcacttcttg gtggtcacca gcgggaagcc
    54661 gtcagccagg ttgaagcgca tctgatgggc aaaaatgctg taggtgccgg tgccggtacg
    54721 gtcacttttg aaggtgccgg tctcgcgcac caagcgcatc aggtcgaggt actgtttcat
    54781 tgcacggcct cctgtgccgc ctgacgtttg taggcatagg cgatcagacc caggccgccg
    54841 agcaccatcg gtacgcagag cacctggccc atggtcagcc agccgccggc caggtaaccg
    54901 agctgggcat ccggcacgcg gacgaattcg acgataaagc ggaagatccc gtagcacacg
    54961 gcaaacatgc cggacaccgc catggtcggc cgtggtttgc gcgagtagaa ccagaggatg
    55021 gcgaacagcg ccacaccttc cagggcgaac tggtagagct gcgatggatg gcgcgccagt
    55081 tgctgcgggt cggtggggaa gatcatcgcc cacggcacgt cggtggcctt gccccacagt
    55141 tcggcgttta tgaagttacc gatacgcccg gcgcccaagc caatcggcac caaaggggcg
    55201 ataaagtcca tcagctcaaa gaagcttttg ttgttgcgct tgccgaacca ccaggtggcc
    55261 agcattacgc cgatcaaccc gccgtggaac gacatgccgc cttcccagat gcgcagcatg
    55321 cgcaccggtt cggcgatata ggccgctagg tcgtaaaaca gcacgtaacc cagacggcca
    55381 ccgaggatta cacccatggc gacccagaac accaggtcgg agagtttctc cttgttccaa
    55441 ctcggtgcga aggtgttgac ccggcgcgaa gccagccacc aggcgccacc gatgccgatc
    55501 aggtacatca ggccgtacca atggattttc agcgggccaa gggccagtgc aaccgggtca
    55561 atctgcgggt aaggcagcat ttcagttcct cagatcagaa aactcaggcc cacgctaaac
    55621 agcagcaggg caaacagacg tttaagcagg cgcggcgaca gcttgtgcgc caggcgtgcg
    55681 ccgacacggg caaagtacat gctggggatg gcaataccga ccagcgccgg caggtagacg
    55741 aaacccagac tccactgcgg cagcgcgggt ttatcccaac ccagccagat aaaactcagg
    55801 gcgctggcta tcgcaatcgg cagaccgcag gccgaagacg tggccaccgc ctgctgcatc
    55861 gctacgctgc gccaggtcag gaacggcacg gtcagcgaac cgccgccaat cccgaaaatc
    55921 gccgaggccc agccaattac accgccggcc agggtcagtg ccggcttgcc tgggacagtg
    55981 cgactggctt tgggtttcag ctccagagcc atttgcacgg cgatgatgat cgcgaatacg
    56041 ccgatgatct tctgcagcag cggcccctga atcgccgtag cggtcatcgc gccgagcccg
    56101 gcgccgagca gaatccccag ggtcatccag gcaaataccg gccacagcac cgcgccttta
    56161 cgctgatgct caattacaga gttgattgag gtgaagatga tggtcgctag cgacgtgcct
    56221 actgccaggt gcgtcaaaat cgccggatca aagccctggg cggcaaaact cagcaccagc
    56281 accggcacga tgataatgcc gccgcccacg ccaaataacc cggcaagcgt tccagctgca
    56341 gccccaagga ccaaatacag cagcagttcc atcagcaccc ccgcaaacaa tccggcatgg
    56401 taacggatgc acagggccgg actctacttt gggcgatgga tgtccgccct ggctttgcgt
    56461 aaagtgcccc catcgcctgt ggaatagacc agcatgcgcc cgaccctgta cctgaaactg
    56521 agctgtaacc agatgcacct gatgcatctg caaagtggcc gcgcgctgca cctgcaagct
    56581 gatccggcgt tcagcaacca gcgcctgcta gtagctgatt ttgccgccgc gcagcaactg
    56641 ctgcagcgcg gtatcaatga actgctgccc aagcgttttc tgcgtctgag ccggccgccg
    56701 caactgctga tccaaccgct ggagcgcctg gaaggcggac tgtcgcaagt cgaggagcgc
    56761 atccttctgg agctcggcct cggctgcggc gcacgcaagg tacgcctgca cctgggcaac
    56821 gaactggata acgccggcgt gctggcgaag ttaaggagct aatccatgtg cctgatcgtc
    56881 ctggcctggc gaccgggcca tgcccagccg ctgctactgg ccgccaaccg cgatgagttc
    56941 tacgagcgcc ccagccagcc cctggccgag tggctcgatc tacccggagt gattgccgga
    57001 cgcgacctgc aggccggcgg cacttggctg ggcgtaggtc caaatgggcg ctttgccgcc
    57061 ctgaccaata ttcgcgaccc cggccaggct caaggcagtc gctcacgggg cgaactgcca
    57121 gtagcctttc tcgccggcag ccacagcgtc gaagattttc tgcatcactt gcacgagcag
    57181 cgtcggcact acagcggctt caacctgctg ctcggtgaca accagcaact ctggcactac
    57241 aactccctga ccggacgcgc cacgccattg gccgaaggcg tacacggcct gtcgaatgcc
    57301 gacctggaca cgccctggcc caagctgcag cgggccaagc aggcgctgca caacgggctg
    57361 catgaaccgc acccgcagat gctgctcaag ctgttggccg accccacccg cgcggaagac
    57421 gctgacttac cgcacaccgg catcggcctg accggcgaac gcctgctgtc cagcatcttt
    57481 attgccagcg ccacctacgg cacgcgcgcc agcaccgcgt tgatcgtcaa tgccgacggc
    57541 agccgcgtgc tggccgaacg cacgttcggc agcaatggca actgtttagg cgaagtggtt
    57601 ttgcagtgtt gaagccgttc agttgcttca ggcagattcg ctgaattggt caagggtaaa
    57661 cagcggcgag gcaatcgtcg gatcgtcggc atccgccacc agcaacggcc ggccatcggt
    57721 ctgaaaggcc aatccctcaa ttttgaccac aggcgccaga cggttcagct cgaggatgct
    57781 cagctgcgca tcgagccgcc ccagcacgct gccaacgcag gcaccgtcca ggtagctgga
    57841 atcggtagcc tccgccgttg cagtgaaata cagcgagcca ttgggcgcca tgctgagatc
    57901 ggtgaggctc agtggcacac cgtccaactc cggcaggtgc agcggcacaa tccgctgcaa
    57961 ggcggcggcg ctcagttgtc catcctgcag atcgcgcagc acctgcggca aatcgagcac
    58021 tactagagca ttttcccgac catggccatt accgcgctgc gccagcagca gctgcccgcg
    58081 atagaccaca ccgccttcga tattcaggtc ctgaaagtgt tcagcgagcg cctgatacaa
    58141 ggggcttagg tcgataacgc gcacgttgtc ggcctcaacc aggcaaccgc gacagcggcg
    58201 cttggtcgaa cccgagccca gggcaagcag gctgtcgtca ggcaataaca acaatgcctc
    58261 gaagtcgggt ttgaggcgtt tgcgctgctt ggcatcgtcg ggcaactcgc ccggcagcag
    58321 cgtcagctcg gcctgccagt cgccactcag gctgtagcgt tgcagcacca gcgcatcgtc
    58381 agccagcacc cagaaactct ggccctgaca caccagtgca ctggccgccg acaagcgcag
    58441 ggttgcgagt tggcgcagct gcagaaagtt atccgcgagg ttcagcacga gcggcgtgcg
    58501 ctcacgcctg gatatccgac gccggattga tcatgcgacc caggccgagg ttgcgcaggg
    58561 ccagttgcag ggtgctgtgg atcacctgcg gactgtcgat ggtcatgatc tgcgccagca
    58621 actcctgggc cttgctcagg ctgatctggc gcagcagcca cttcactttg ggcaggttgg
    58681 tggcgttcat cgacaggccg tcgaaaccca gggccatcag caacacggcg cacgccggat
    58741 caccggccat ttcaccgcag atgctcaccg gtttgccttc agcatgcgca ccatcgacca
    58801 cgatacgcag cgcctgcagc acggcaggat ggaggaagtc gtagagatca gcgacgcgcg
    58861 ggttattacg gtccaccgcc aacaggtatt gggtcaggtc attcgagcct accgagagga
    58921 aatcgacttg gcgtgccagt tcgcgcacct gatagaccgc cgcaggaatc tcgatcatca
    58981 ccccgaccgg tggcagcggc acgtcggtgc cttcgtcacg cacctcgccc caggcacggt
    59041 gaatcaggtg cagcgcttct tccaactcct gaatcccgga aatcatcggc agcaggatgc
    59101 gcaggttgtt cagcccctcg ctggccttga gcatggcgcg ggtctgcacc aggaagattt
    59161 ccgggtggtc gagggtgacg cggatgcctc gccagccgag gaaggggttg tcttccttga
    59221 tcgggaagta gctcagtgcc ttgtcgccgc cgatatccag gctgcgcatg gtcaccggca
    59281 gcgggtggaa ggcggccaat tgctcgcgat aaatcgccag ctgctccttc tcgctgggga
    59341 agcgctcctt gatcataaac ggcacttcgg tgcggtacag gccaacccct tcagcaccgc
    59401 gctcctgggc gcgttttacg tcggcgagca ggccggtgtt gacccacaac ggcatgcggt
    59461 ggccatcgag ggtttcgcac ggcaaggcgc gcagcgcgtc gagaccttgg gccagctggc
    59521 gctcttcctc gaccacttcg gcgtattgct tgcgcaacac ttcgctgggg ttggtgaaca
    59581 cctcgccgtg gtagccgtcg acgatcagct gaatgccgtc gatcttcgaa tacggcagat
    59641 cgaccacgcc catcaccgtg ggaataccca tggctcgggc aaaaatcgcc acatgggagt
    59701 tgcccgagcc ctgcaccgaa accaggccga ccagcttgcc ttccggcacc tcaccgagca
    59761 tcgccggcga cagttcttcg ctgaccagga tgcagttgtc cgggtacacc agggtctgct
    59821 ggcgcgcctg ctgcagatag gcgagcaggc gccggccgag gtctttgacg tcgctggcgc
    59881 gctcgcgcag gtaggcgtcg tccatcagtt cgaaacggtt gatgtgctcg ccaatcacct
    59941 ggcgcagtgc gccctgggcc cactggccgg tgcggatgac cttgaccacc tcgttaccca
    60001 gagcggcgtc ttccagcatc atcagataca cgtcgaacag cgcacgctct tcggggcgca
    60061 gctgggtggc catcttcgcc gacagcgcgc gcatgtcgct gcgcacacct tccagggcgt
    60121 tattgaacag ggtcagctcg gcggcgatgt cgtcgacggt tttatccggc accacttcca
    60181 ggtcggccgg cggcaatacc accaccgctg taccaaccgc tgcgccgggc gagcctggca
    60241 cgccgacgaa cttggcttcc tgaatgccct tgccctgccg gcccaaaccg cgaatcgagc
    60301 cggtggcctc agcgtgggcg ataaccccgg cgagctgcgc gctcatggtg accaggaagg
    60361 cttcttcgcc ctcatcgaat tggcgctgct ctttctgctg gataaccagc acgcccatca
    60421 ccttgcggtg gtgaatgatc ggcgcaccga gaaaggaggc gtaacgctcc tcgccggtct
    60481 cagcaaagta gcggtagcgt gggtgttcgg aggcgtgttc gaggttgagc ggttcttcgc
    60541 gggtaccgac cagaccgacc aaaccctcgt tgggggccat gctgaccttg ccaatggcct
    60601 tcttgttcag accctcggtg gccatcagca caaagcggtt gctttccggg tcaagcaggt
    60661 agaccgagca gacctggctg cccatggcct cgcgcacccg ctgcacgata atgcccagcg
    60721 ccgccttgag gtccttggcg gcatttactt cctggacaat cttgcgcagc gtattgagca
    60781 tgcttggctc tagcctgtgt cagtcgcgcg ccaaaaggcg cggagcgagt tctttgaggg
    60841 cgcgacggta aacctcgcgc ttgaatgtca ccacctggcc cagcgggtac cagtaactga
    60901 cccagcgcca gccgtcgaac tccggcttgc cggtcaggtc catgcgcacc ctatcttctg
    60961 cgccggtcag acgcaggaga aaccacttct gtttctgccc gatgcacagc ggttggctgt
    61021 gtgtgcgcac caggcgctga ggcagacgat aacgcaacca gccccgggtg caggcgagaa
    61081 ttttcacatc ctgctgttcc aggcccactt cttcattcaa ttcgcggtac agcgcttctt
    61141 ccggtgactc atggtcattg atcccgccct gcgggaactg ccaagcgtcc tggtttatcc
    61201 gacgcgccca cagcacctgg ccgacatcat tggtcagaat gatgccgaca ttcgggcgaa
    61261 aaccatcgga atcgatcacg gcacac
//
LOCUS       DASRUM010000052         7111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2033891, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000052 DASRUM010000000
VERSION     DASRUM010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7111
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2033891"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFF22_03970"
     CDS             <1..253
                     /locus_tag="VFF22_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZX16012.1"
                     /translation="KQLEILIAADGSYSLNAKQLLKSDLESLMAALQKESEGDNSLPL
                     IISADAKAQHQAVITAMDAAGKLGFAHLRMTTIEAQAAP"
     gene            267..1268
                     /gene="lpxK"
                     /locus_tag="VFF22_03975"
     CDS             267..1268
                     /gene="lpxK"
                     /locus_tag="VFF22_03975"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HZX16013.1"
                     /translation="MSLSARLTAAWYAGHPALGLLRPLEWLYRAVVQRKRARFLAGEG
                     EIYRAPVPVVVVGNITVGGTGKTPMILWLIEHCRRQGLRVGVVSRGYGAQPPQLPWRV
                     QADQAASVAGDEPLLIVQRSGVPLMIDPDRGRAVAALLAAEELDLILSDDGLQHYRLA
                     RDLELVLIDAARGLGNRHCLPAGPLREPVERLQSVDALLYNGASSDPHDGYGFTLQPR
                     TLVNLRSGEQHPLSHFPAGQALHAVAGIGNPQRFFNTLEGLHWRPVAHAFADHAPYSA
                     ELLDFSPELPVLMTEKDAVKCRAFAADDWWYLAVDAEPSAAFVAWFDQQLQRLVPRA"
     gene            1330..1515
                     /locus_tag="VFF22_03980"
     CDS             1330..1515
                     /locus_tag="VFF22_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HZX16014.1"
                     /translation="MDPKLLDILACPLCKGPLKLAADKSELICKADALAFPVRDGIPV
                     MLETEARTLNVDERLDK"
     gene            1516..2280
                     /gene="kdsB"
                     /locus_tag="VFF22_03985"
     CDS             1516..2280
                     /gene="kdsB"
                     /locus_tag="VFF22_03985"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HZX16015.1"
                     /translation="MSTAFTVVIPARFASSRLPGKPLQDIAGKPMIQHVWEQACKSSA
                     QQVVVATDDARIVEVCRSFGAQVVLTRVDHNSGTDRLAEVATALGLAADAIVVNVQGD
                     EPLIPPTIIDQVAANLAANPQAAIATLAEPIEDVQALFNPNVVKVVSDKTGLALTFSR
                     APLAWARDAFAKSRDELPAGVPYRRHIGIYAYRAQFLHDFVVWGPCWLEDTECLEQLR
                     ALWHGVRIHVADALEAPPAGVDTPEDLERVRRLLGA"
     gene            2280..2744
                     /locus_tag="VFF22_03990"
     CDS             2280..2744
                     /locus_tag="VFF22_03990"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HZX16016.1"
                     /translation="MRVLFVCLGNICRSPTAEGVLRHKLREAGLEGRIEVDSAGTGDW
                     HVGKAADLRTRQAAQQRGYDLSALRGRQVKAADFHAFDLILAMDNSNLHDLQQLRPGN
                     DAAELDLFLRRYQLELDEVPDPYYGGEAGFAQVLDLIEQACDALVIELKGRL"
     gene            2741..3760
                     /gene="murB"
                     /locus_tag="VFF22_03995"
     CDS             2741..3760
                     /gene="murB"
                     /locus_tag="VFF22_03995"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HZX16017.1"
                     /translation="MSLQVRTQVSLKPYNSFAVEAQASHFAEAENDQQVREALSYAHT
                     QQLPLLLLGGGSNLLLTADVEALVLRMASRGIRVLSDDGERVVIEAEAGEPWHPFVQW
                     TLAQGFAGLENLSLIPGTVGAAPVQNIGAYGVELKDLFAGLTVLDRRSGELREFSLQD
                     CAFAYRDSLFKRESGRWVILRVRFALSRAAALHLDYGPVRQRLAEQGISAPTALDVSQ
                     AICAIRSEKLPDPAELGNAGSFFKNPLVSSELAQHLRAEHADLVAYAQADGQVKLAAG
                     WLIERAGWKGFREGDAGVHRLQALVLVNYGDASGAQLLALAQRIQADIAQRFAVDLEI
                     EPNVL"
     gene            complement(3865..6984)
                     /gene="rne"
                     /locus_tag="VFF22_04000"
     CDS             complement(3865..6984)
                     /gene="rne"
                     /locus_tag="VFF22_04000"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HZX16018.1"
                     /translation="MKRMLINATQPEELRVALVDGQKLYDLDIESGAREQKKSNIYKG
                     KITRVEPSLEAAFVDFGSERHGFLPLKEISREYFSKAPEGRVNIKDVLKEGQEVIVQV
                     EKEERGNKGAALTTFISLAGRYLVLMPNNPRAGGISRRIEGEERNELREALNGLVAPS
                     DMGLIVRTAGLGRSSEEMQWDLDYLLQLWTAIKEASLDRAAPFLIYQESNVIIRAIRD
                     YLRQDIGEVLVDSIEAQQEALSFINQVMPQYASKIKLYEDSVPLFNRFQIESQIETAF
                     QREVKLPSGGSIVIDPTEALVSIDINSARATKGSDIEETALQTNLEAAEEIARQLRLR
                     DIGGLIVIDFIDMTPAKNQRAVEEKVRESLEADRARVQVGRISRFGLLEMSRQRLRPS
                     LGESSGIVCPRCNGQGIIRDVESLSLAILRLIEEEALKDRTAEVRAQVPIPVAAFLLN
                     EKRNSITKTELRTRARIVIIPNDHLETPHFEVTRIRDDSPEAQSTQSSYEMATVEVEE
                     EKQAAATRTLVRQEAAIKTAPQRTAPTPVEAVAAQPVAAAEPSLFKGLVKSLVSLFAS
                     KEEEKPAVVEKKTSERPQRSDERRNGRQQNRSRGGRNNEERKPREERGPREDRAPREE
                     RQPRAAREERAPREERAPREERAPREERSPRDVVEVREPQEVRQSRPPREERQPRDRK
                     PREERQPRELREPLDAAPGDSATVSEEARAERPQREPRQPRAQREERQPRAEQAAVAA
                     DEEVLNAEEALQDDEQEGSEGGDRPRRRSRGQRRRSNRRERQNDANGNPIEGSEEGAE
                     APADADNTVVSAAVTAAVITGADEAVQATAEAAPVVVEQEQPREIAAAETPQAVVETV
                     AVEAVSSPVVEVSEAPVYATEVSEAAPAPAVVEAPAPVAEVVEAPAVVTAPEPVAEAV
                     VAEVVAAEAAPVVAASSTGRAPNDPREVRRRQREAERLVREAAEAPAASVQVEAAAPV
                     AEEPAAEVAAEAVVETAAEAPASIEAVEPKAEAAAQVEAEVNQEPQSSLFTEVEADEQ
                     DKEAVKPQV"
BASE COUNT         1151 a         2080 c         2293 g         1587 t
ORIGIN      
        1 gaagcagctg gaaatcctga ttgccgccga tggcagctac tcgctgaatg ccaaacagtt
       61 gctgaaaagc gatctggaaa gcctgatggc agcgctgcag aaggaatcgg aaggtgataa
      121 cagcctgccg ctgatcatca gcgccgatgc caaggctcag catcaggcgg tgattaccgc
      181 catggatgcg gccggcaaac tgggttttgc ccacctgcgc atgaccacca tcgaggcgca
      241 agccgcgcct tgagggttgc ccactgatga gcctgtccgc acgactcact gcagcctggt
      301 acgccggtca cccggcgctg ggtttgctgc gtccgctgga gtggctgtat cgcgcggtgg
      361 tgcagcgtaa gcgtgcgcgt tttctggccg gtgagggcga gatctaccgt gctcccgtac
      421 cggtagtggt ggtcggcaat atcactgtcg gcggcaccgg caaaaccccc atgattctct
      481 ggctgatcga gcactgccgc cgccaagggc tgcgcgtcgg tgtggtcagt cggggctatg
      541 gcgctcagcc gccgcagctg ccctggcgtg tgcaggccga tcaggctgcc agcgtggccg
      601 gtgatgagcc gctgcttatt gtgcaacgca gtggcgtgcc gctgatgatt gatcctgatc
      661 gcggccgcgc cgtggcggct ttgctggcgg ccgaggagct ggacctgatc ctcagtgacg
      721 acggtttgca gcactaccgc ctggcccgcg atcttgaact ggtgctgatc gatgccgcgc
      781 gcggcttggg caatcgccat tgcctgcctg ccggcccgct gcgagaaccg gtcgagcgtc
      841 tgcagagcgt ggatgcgctg ctgtataacg gcgcgtccag cgatccgcac gatggctacg
      901 gctttaccct gcagccgcgt accttggtca acctgcgcag tggcgagcag catccgctca
      961 gtcacttccc ggccggtcag gccctgcatg cggtcgccgg tattggcaac ccgcagcgtt
     1021 tcttcaatac cctcgaaggg ctacactggc gacctgtcgc gcatgccttt gccgaccatg
     1081 ccccgtatag cgccgagctg ctggatttta gcccggagct gccggtgctg atgacggaaa
     1141 aggatgcggt caagtgccgt gcctttgcgg ctgacgattg gtggtacctg gcggtggatg
     1201 ccgagccttc ggccgcgttt gtcgcttggt ttgatcagca attgcagcgt cttgtgccgc
     1261 gcgcctgact gttctgatca ttcggcggcc tcgaccgccc cagcagctgc tccctatcaa
     1321 ggatctccca tggacccgaa actactcgac attctcgcct gcccactgtg caagggcccg
     1381 ctgaagctcg ccgctgacaa gagtgagctg atctgcaagg ccgacgcact ggctttcccg
     1441 gttcgcgatg gcatcccggt gatgctggaa accgaagccc gcaccctcaa cgtcgacgag
     1501 cgcctggaca agtaaatgag cactgccttt accgttgtga ttccggcgcg ttttgcttcc
     1561 agccgcctgc cgggcaagcc gctgcaggat attgccggca aaccgatgat tcagcatgtc
     1621 tgggaacagg cgtgcaaaag cagcgcccag caggtggtag tcgccaccga cgatgcgcgc
     1681 atcgttgagg tctgccgcag ctttggtgcg caggtggtgc tgacccgcgt cgaccataac
     1741 tccggtaccg accgcctggc tgaagtagcc acggctctgg gcctggcggc tgatgccatc
     1801 gtggtcaacg tgcagggcga tgagccgctg attcctccca cgatcattga tcaggtggcg
     1861 gccaacctgg cggccaaccc acaggcggca atcgccactt tggctgagcc gattgaggat
     1921 gtgcaggcgt tgttcaatcc caatgtggtc aaggtggtca gcgacaagac cggcctggcc
     1981 ctgaccttca gccgtgcgcc actggcctgg gcgcgggatg cctttgccaa aagccgggac
     2041 gagctgccag caggcgtacc gtaccgtcgc cacatcggca tctacgccta ccgtgcgcag
     2101 tttctgcatg acttcgtcgt ctggggtccg tgctggctgg aagacaccga gtgcctggag
     2161 cagctgcgcg cgctctggca tggcgtgcgt atccatgtcg ccgatgccct ggaggcgcca
     2221 cctgcgggcg tcgacacgcc agaagacctg gagcgggttc gtcgcctgct gggggcctga
     2281 tgcgcgttct gtttgtctgc ctgggcaata tctgccgctc gccaacggcc gaaggcgtgt
     2341 tacgccataa gctgcgtgaa gcggggctgg aagggcgcat cgaagtagac tcggccggca
     2401 ctggcgactg gcacgtgggc aaagcagcgg atctgcgcac gcgccaggct gcgcagcagc
     2461 gtggctatga cctgtctgcg ctgcgcggtc ggcaggtcaa ggcggcggat tttcatgcct
     2521 tcgacctgat tctggcgatg gataacagca acctgcacga cctgcagcag ctgcgtccgg
     2581 gcaatgatgc ggccgagctg gacctgtttc tgcggcgtta tcagctggaa ctggatgaag
     2641 tgccggaccc ttattacggt ggtgaagccg gttttgccca ggtgctggat ttgatcgaac
     2701 aggcctgtga tgccctggtt attgaactca aggggcgctt atgagcttgc aggtgcgcac
     2761 tcaggtctcg ctaaagccct ataacagctt tgccgtcgag gcccaggcca gccattttgc
     2821 cgaagctgag aacgatcagc aggtgcgcga agcgctgagc tatgcgcaca cacagcagtt
     2881 gccactgctg ttgctcgggg gtggcagcaa tctgctactc accgctgacg ttgaggcgct
     2941 ggtgctgcgt atggcgagcc gcggtattcg cgtactgagc gatgacggcg agcgtgtggt
     3001 gatcgaggca gaggcggggg agccctggca tccgtttgtg caatggacgc tggcgcaggg
     3061 ctttgccggc ctggaaaacc tcagcctgat ccccggcaca gttggcgcgg cgccggtgca
     3121 gaatattggt gcgtacggcg ttgagctgaa ggatctgttt gccggtttga ccgtcttgga
     3181 tcggcgcagt ggtgagctgc gcgagttctc tctgcaggac tgcgcctttg cctaccgcga
     3241 tagcctgttc aaacgcgaaa gcgggcgctg ggtcatcctg cgggtgcgct ttgccttgag
     3301 ccgtgcggct gcgctgcatc tggattacgg cccggtgcgt cagcgtctgg cggagcaggg
     3361 catcagtgct ccgacggcgc tggatgtcag ccaggccatc tgcgccattc gcagcgaaaa
     3421 gttgcctgac ccggctgagc tgggcaatgc cggcagcttc ttcaagaacc cgttggtatc
     3481 gagtgagctg gcgcagcatt tgcgcgccga acatgccgat ctggtggcgt atgctcaggc
     3541 agacgggcag gtaaaacttg ccgctggctg gttgatcgag cgtgcgggct ggaagggctt
     3601 tcgtgagggt gatgcgggcg tgcatcgtct gcaggcgctg gtattggtga attacgggga
     3661 cgccagcggt gcgcagttgt tggcactggc gcagcgtatc caggcggata tcgcccagcg
     3721 ttttgcggtg gacttggaga tcgagccgaa cgtgctgtaa agcgttttgg cggaagctag
     3781 ctaacagcgt gttgatcgcg ctgtaataaa agctacaacg caaaagggga tgccgcagca
     3841 tccccttttt tattgcctag cggatcagac ctgcggtttg accgcttctt tatcctgctc
     3901 gtcagcttct acttcagtaa acagtgagct ttgcggctcc tggtttactt ctgcttctac
     3961 ttgcgcggcg gcttcggcct tgggctcgac tgcttcgatg ctcgcaggtg cttcggctgc
     4021 agtttcaact acggcttcgg cagccacttc tgcagctggc tcttcagcta ccggtgcagc
     4081 agcttcaacc tgtacggagg ctgctggggc ttcggcggct tcgcggacca gacgctcggc
     4141 ttcacgctga cgacgacgca cttcgcgcgg atcgttaggt gcgcggccgg tgctgctggc
     4201 ggcaaccaca ggggcagctt cggctgctac aacttcagct actacggctt cggcaaccgg
     4261 ctcgggtgcg gtaaccacgg caggcgcttc aacaacctct gcaaccgggg ctggcgcctc
     4321 tacaacagca ggtgctggtg cagcttcgct gacttcggtg gcatatacag gtgcttcgct
     4381 gacttcgaca accgggctgc ttactgcttc tacagctacg gtctcaacaa cggcttgtgg
     4441 cgtttcagct gcagcgattt cgcgaggctg ctcctgctcg acaactaccg gtgcggcttc
     4501 ggcggttgct tggacggctt cgtctgcgcc agtgataacg gcggctgtta ctgctgctga
     4561 aactactgtg ttgtcggcat cggctggtgc ttctgcgcct tcttcactgc cttcaatcgg
     4621 attgccgttg gcatcgttct ggcgctcgcg acgattgctg cgacgacgct gaccacggga
     4681 gcggcgacgt gggcgatcgc caccttcgct gccttcctgc tcatcgtcct gcagtgcttc
     4741 ttcagcgttc agtacttctt catcagcggc tactgcggcc tgttcggcgc gtggctggcg
     4801 ctcttcacgc tgtgcgcgcg gttggcgcgg ttcgcgttgc gggcgttcgg cacgggcttc
     4861 ttcgctgacg gttgcgctat caccaggtgc ggcatccagt ggctcacgca gttcgcgcgg
     4921 ctggcgctct tcacgtggct tgcgatcgcg cggctgacgc tcttcgcgcg gtgggcgcga
     4981 ctgacgaact tcctgcggct cgcgaacttc tacgacgtca cgcgggctgc gttcctcgcg
     5041 tggtgcacgc tcttcgcgcg gtgcgcgttc ctcacgtgga gcgcgttctt cacgggctgc
     5101 acgaggctga cgctcttcac gcggtgcgcg gtcctcacgt gggccacgct cttcgcgcgg
     5161 cttgcgctct tcgttgttgc gaccaccacg gctgcggttc tgctggcgac cgttgcgacg
     5221 ctcatcgctg cgctgtggac gttcgctggt tttcttctcg acgacggcag gcttttcttc
     5281 ttccttgctg gcgaacagac tgaccagcga cttgaccagg cccttgaaca ggcttggttc
     5341 agcggcagct acaggctgtg cggctactgc ttcaaccggg gttggtgcag tgcgttgcgg
     5401 cgcggtcttg atcgcggctt cctggcgaac caaggtgcgc gttgcggcgg cctgcttctc
     5461 ttcttccact tcaacggtcg ccatttcata gctggactga gtggactgag cctccggact
     5521 gtcgtcacgg atacgggtga cttcgaagtg cggggtttcc aggtgatcgt tcgggatgat
     5581 tacgatacgt gcacgggtac gcagctcggt cttggtgatc gagttgcgtt tttcgttgag
     5641 caggaatgcg gcaactggaa ttggcacttg tgcgcgaact tcagcagtgc ggtctttcag
     5701 ggcttcttct tcgatcaggc gcagaatggc caacgacagc gattcgacgt cgcggatgat
     5761 gccttgacca ttgcagcgtg ggcagacgat accgctgctt tcgcccaggg atggacgcag
     5821 gcgctgacgc gacatttcca gcaggccgaa gcgcgagatg cgacccactt gtacgcgggc
     5881 acggtcggct tccaggcttt cacggacttt ttcttccacg gcgcgttggt ttttcgccgg
     5941 ggtcatgtcg ataaagtcga taacgatcag gccgccgatg tcacgcagac gcagttggcg
     6001 ggcaatttct tcagccgctt ccaggttggt ctgcagggcc gtttcttcga tgtcgctgcc
     6061 tttggtcgca cgcgccgagt tgatgtcgat ggacaccagg gcttcggtcg ggtcgatgac
     6121 gatggagcca ccggacggca gtttcacttc gcgctggaag gcggtttcga tctggctttc
     6181 gatctggaag cggttgaaca acggcacgct gtcttcatac agcttgatct tgctggcgta
     6241 ctgcggcatc acctgattga tgaaactcag ggcttcctgc tgcgcttcga tgctgtcgac
     6301 cagtacttcg ccgatgtcct ggcgcaggta gtcgcgaatt gcgcggatga tcacgttgct
     6361 ttcctggtag atcaggaagg gcgcagcgcg gtccagcgag gcttctttga tagcggtcca
     6421 gagttgcagc aggtaatcga ggtcccactg catttcttcg ctggagcggc ccagacctgc
     6481 agtgcgcacg atcaagccca tgtcgcttgg agcgaccagg ccgttcaggg cttcacgcag
     6541 ctcattacgc tcttcgcctt cgatacgacg cgagatacca ccggcgcgcg ggttgttcgg
     6601 catcagtacc aggtaacgac cggcgaggct gataaaggtg gtcagggctg cgcccttgtt
     6661 gccacgctct tctttctcga cctgaacgat tacttcttgg ccttccttga gcacgtcttt
     6721 gatattgacg cggccttctg gagccttgct gaagtattcg cgggagattt ctttgagcgg
     6781 gagaaaacca tggcgctcag agccgaagtc gacgaaggcg gcttccaggc taggttctac
     6841 gcgggtgatt ttacccttat agatgttgga ttttttctgc tcacgagcgc cggactcgat
     6901 gtccaggtcg tagagtttct gcccatctac cagggcaaca cgcaactctt cgggctgagt
     6961 tgcgttaata agcattcttt tcatgtagta ccgtcggttt ccggtgctgg cggaagcggc
     7021 gttcggcaca cacgacttca ggggtcggtg tcaggtgcgt caggaacggt cgtaaagcgt
     7081 tccagtgtct agcggcggtc agccaattgt g
//
LOCUS       DASRUM010000053         4510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2198225, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000053 DASRUM010000000
VERSION     DASRUM010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2198225"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFF22_04005"
     CDS             <1..358
                     /locus_tag="VFF22_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HZX16019.1"
                     /translation="LEACASYRKGLPLMVLALADNSRELRLADAADVPLMFEAFADRA
                     YLADGQLAPRRLSGAVHHDPQRIFQQALAIARGEPFADIDGKPLQLRADSLCVHGDNA
                     DSLAVLRRLRASLDAL"
     gene            355..1032
                     /gene="pxpB"
                     /locus_tag="VFF22_04010"
     CDS             355..1032
                     /gene="pxpB"
                     /locus_tag="VFF22_04010"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019372154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HZX16020.1"
                     /translation="MIRLEPAGAEALLLVLAEQPDAQLPQRIALLAERIRGELGEVLT
                     DLVPGWTSLLLHYDLLRTDHLELAERLKPLLERWLAEPFVAQAGRLHEIPILYNGEDL
                     AEVARLCQLSSAQVIELHAATEYRVGAIGFAPGFAYLGELDARLALPRRATPRISVPA
                     GSLAIAERQTAIYPHSSPGGWHLIGRCPWSLFDAARTPPCPLALGDRVRFRSIDERDF
                     IREGGQL"
     gene            1029..1931
                     /locus_tag="VFF22_04015"
     CDS             1029..1931
                     /locus_tag="VFF22_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HZX16021.1"
                     /translation="MSSLRVIKPGPQSLLQDAGRQGWQHLGVSPAGPLDWHGAAWANH
                     LLNNPWGTPLLEIALGGVELQAEVDTWVAVCGAQVPLSLDDQPQPLWTRLPLRKGQLL
                     RMGFARSGQRAYLAVAGGFNAAPVLGSVSVQLREGLGQALQAGDLLVCRPAHFSRCVS
                     VPWPYLPDYACKPLLRVITGGDAASFEEDQLQSFFAQTWQLSPHSDRMGARLLGEAVQ
                     PPVRQWSLGVGRGAIQVPPDGQPIILQADHQSMGGYPLLGWLHPLDQGRLAQCPAHHP
                     LRFTPISVGDAQAELREFYRFFRR"
     gene            complement(1928..3448)
                     /gene="lnt"
                     /locus_tag="VFF22_04020"
     CDS             complement(1928..3448)
                     /gene="lnt"
                     /locus_tag="VFF22_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HZX16022.1"
                     /translation="MHWITRPGWPGNLIALLAGAITPLALAPYDLWPLAILSIALFYL
                     GLRDLTPRQAALRGWSYGFGMFAAGTSWVYVSIHDYGAASPPLAVFLTLLFVGGLGLL
                     FALSAWVWTRWLRRVEAPLADALAFSALWLAQEAFRSWFLTGFPWLYAGYSQLDGPLA
                     GLAPVGGVWLLSFALALSAALLVNLAQLRTRKAFLVIGVVLLIAPWIAGIALKGHAWT
                     QPLGEPLSVSAMQGNIEQNMKWDPEQLNAQLALYRDMTFSSKPTDLIVWPETAIPVLK
                     DRAQGYLGMINRFANERNAALITGVPIRQSNERGEQRYYNGISVVGQGSGDYLKQKLV
                     PFGEYVPLQDLLRGLIAFFDLPMSDFARGSAEQSLLQAKGYSIAAFICYEVVYPEFSA
                     GLAAQSDLLLTISNDTWFGRSIGPLQHLQMAQMRALEAGRWMIRATNNGVTALIDPQG
                     QITTQLPQFEQGVLYGQVQPMQGLTPYLYWRGWPMLIICLLLFSWALLASRMAKTL"
     gene            complement(3685..>4510)
                     /locus_tag="VFF22_04025"
     CDS             complement(3685..>4510)
                     /locus_tag="VFF22_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HZX16023.1"
                     /translation="SSNEQKSWFNRLTQAFAHEPKNRKELLEVLREAHQNKLLDSEAL
                     AIVEGAIQVADLQVRDIMVPRSQMMSIKANQTPKEFLPSIIEAAHSRYPVVGESLDDV
                     IGILLAKDLLPLILSGEQPNFNIKDLLRPATFVPESKRLNVLLREFRANHNHMAVVID
                     EYGGVAGLVTIEDVLEQIVGDIEDEHDVEEDSYVKPLPSGDFLVKALTPIESFNEAFD
                     TEFSDDEFDTVGGLVMSAFGHLPKRNEVTQIGEFRFRVLNADSRRIHLLRLSPVSN"
BASE COUNT          787 a         1354 c         1531 g          838 t
ORIGIN      
        1 gcttgaggcc tgcgccagtt accgcaaggg cttgccgctg atggtgctgg cgctggccga
       61 caacagccgt gagctgcgcc tggccgatgc cgccgatgtg ccgttgatgt tcgaggcctt
      121 tgccgaccgt gcttacctgg ccgatgggca gttggcaccg aggcgtttga gcggcgcggt
      181 gcaccatgat ccgcagcgga ttttccagca ggccctggcg attgcccgcg gtgagccgtt
      241 tgccgatatc gatggaaagc cgctgcagct gcgcgccgac agcctctgcg tgcatggcga
      301 caacgccgac tccctggccg tactgcggcg tttgcgggcc agcctggacg ccctatgatc
      361 cgcctcgaac cagccggtgc tgaagcgctg ctgctggtgc tggccgagca gccggatgcg
      421 cagctgccgc agcggatcgc gttgctggcc gagcgtatcc gtggcgagtt gggtgaggtg
      481 ctcaccgatc tggtgccggg ctggaccagc ctgctactgc actacgacct gctgcgcacg
      541 gatcacctgg aattggccga gcggctcaag ccgctgctgg agcgctggct ggccgaaccc
      601 tttgtcgcgc aggccgggcg gctgcatgaa atccccattt tgtataacgg tgaagacctc
      661 gccgaggtcg cgcgtctgtg tcagctgagc agcgctcagg tgatcgaact gcatgcggcg
      721 accgagtacc gcgttggtgc gattggtttt gctccggggt ttgcctacct gggtgagctg
      781 gacgcgcgcc tggccttgcc acgccgcgcc accccgcgca tctcagtacc ggccgggagt
      841 ctggcgattg ccgagcggca gacggcgatc tacccgcata gctcgcccgg cggctggcac
      901 ctgatcggcc gttgtccctg gtcgctgttc gacgccgcga gaacaccgcc atgtccattg
      961 gcactgggtg atcgggtgcg ttttcgcagc attgatgagc gcgactttat ccgcgaaggc
     1021 ggtcagttat gagcagcctg cgggtgatca aaccagggcc gcagagtctg ttgcaggatg
     1081 ctggacgtca ggggtggcag cacctgggtg tatcgccagc ggggccgctg gattggcacg
     1141 gcgcggcctg ggccaatcat ctgctgaaca atccctgggg cacgccgctg ctggaaatcg
     1201 ccctgggcgg cgtcgagctg caggccgagg tcgacacctg ggtggcggtt tgcggcgccc
     1261 aggtgccgtt gagcctggat gatcagccgc agccattatg gacacgcttg ccgctgcgca
     1321 aaggacagct gctgcgtatg ggctttgccc gcagcggcca gcgcgcctat ctggcggtgg
     1381 ccggcggctt taatgcggcg ccggtgctgg gcagcgtcag cgtgcagctg cgcgaagggc
     1441 tggggcaggc tttgcaggca ggggatctgc tggtttgtcg gccggcgcat ttcagccgct
     1501 gcgtcagcgt gccctggccc tatctgccgg attacgcctg caaacccttg ctgcgtgtga
     1561 ttaccggcgg cgatgcggcg agtttcgaag aagaccaact gcagagcttt ttcgcccaga
     1621 cctggcagct cagcccgcac tctgatcgca tgggcgcacg gctgctcggc gaggccgtgc
     1681 agccgcccgt gcggcaatgg tcattggggg ttggccgggg ggcgattcag gtgccgccgg
     1741 atggccagcc gatcatcctg caggccgacc accagagcat gggcggttac ccgctgctcg
     1801 gttggctgca tccgctcgat caggggcgcc tggcgcaatg cccggcgcac catccgctgc
     1861 gtttcacgcc aatcagcgtc ggtgatgcgc aggccgagct gcgcgagttc taccgattct
     1921 ttcgtcgtta aagcgttttg gccatacggc tggccagcaa ggcccaggaa aacagcagca
     1981 ggcagataat cagcatcggc cagccacgcc agtacaggta aggcgtaagc ccctgcatcg
     2041 gttgcacctg gccatagagc acgccctgtt cgaactgcgg cagttgcgtg gtgatctggc
     2101 cttgcgggtc gatcagcgcg gtgacgccgt tgttggtggc gcggatcatc cagcggccgg
     2161 cttccagggc gcgcatctgc gccatctgca ggtgctgcag cgggccgatg gagcgaccga
     2221 accaggtgtc gttgctgatg gtcagcagca ggtcgctctg cgccgcgagg ccggcggaaa
     2281 actctgggta gaccacttcg tagcagataa acgcggcaat cgaatatccc ttggcctgca
     2341 acaggctttg ctcggcggag cctcgggcaa agtccgacat cggcaggtcg aagaaggcga
     2401 tcagcccgcg cagcaggtcc tgcagcggca cgtattcacc gaaaggcacc agcttctgct
     2461 tgaggtagtc gccgctgccc tggccgacca cgctgatgcc gttgtagtag cgttgttcgc
     2521 cgcgctcgtt gctctggcga attggcacgc cggtaatcag cgcggcattt cgctcattgg
     2581 cgaagcgatt gatcatgccc aggtagccct gggcccggtc cttgagcacc ggaatggcgg
     2641 tttccggcca gacgatcagg tcggtcggct tggagctgaa ggtcatgtcg cggtacagcg
     2701 cgagctgcgc gttgagctgc tcggggtccc atttcatgtt ctgttcgata ttgccctgca
     2761 tggccgacac gctcagcggc tcgcccagcg gctgggtcca ggcgtggccc ttgagagcga
     2821 taccggcgat ccacggggcg attaacagca ccacgccgat gacgagaaac gccttgcgcg
     2881 tgcgcagttg cgccaggttc accagtagcg cggcgctgag cgccaaggca aacgacagca
     2941 gccacacgcc gcccaccggg gccagcccgg ccagtggacc gtcgagctgg ctgtaaccgg
     3001 catacagcca ggggaaaccg gtgagaaacc agctgcggaa ggcctcttgt gccagccata
     3061 gcgcggagaa agccagggca tcggccagtg gcgcttcaac ccggcgcagc cagcgcgtcc
     3121 agacccaagc actgagggcg aatagcaagc ccagaccgcc aacaaacagc agggtcagaa
     3181 ataccgccag gggcggcgag gccgcaccgt aatcgtggat gcttacgtac acccagctgg
     3241 tgccggcagc gaacatgcca aagccataac tccagccgcg cagcgccgcc tgacgcggcg
     3301 tgaggtcacg caggccgagg tagaacaggg cgatggagag gatggccagc ggccacaggt
     3361 cataaggcgc cagcgccagg ggcgtgatag cgcctgccaa cagcgcgatc aagttgccgg
     3421 gccagccagg tcgggttatc cagtgcattg catgcaggtc cttggattga ggtggcgcaa
     3481 gggtaaagcg ggagggcaga cgggggaagt gttgcgaggc ttgcttgaga gcctgcttac
     3541 gatctcgcga gttagagcca tgcaaggcaa aaacaggcga ggaagcgcag tgtacgagtt
     3601 gtacatgagc attccgagcc tgtttttaac gccgcagggc cgacgagcag cagatcgtaa
     3661 acaggctctg tggccggcta tgggtcagtt gctaaccggg ctcaggcgca gcagatggat
     3721 gcggcggctg tcggcgttga gtacgcggaa gcggaattcg ccgatctggg tgacttcgtt
     3781 gcgcttgggc aggtggccga aggcgctcat caccaggccg ccgacggtgt cgaactcgtc
     3841 atcagagaat tcggtatcga aggcttcgtt gaagctttca atcggcgtca gcgccttgac
     3901 caggaagtcg ccggagggca gcggtttgac gtagctgtct tcttccacgt cgtgttcgtc
     3961 ttcgatatcg ccgacgatct gctcaagcac gtcctcgatg gtcaccaggc cggcaacgcc
     4021 gccgtactca tcaatcacca cggccatgtg gttgtggttg gcgcggaact cgcgcagcag
     4081 cacgttgagg cgcttggact cgggcacgaa ggtggccggg cgcagcaggt ccttgatatt
     4141 gaagttgggt tgttcgcccg agaggatcag cggcagcaga tccttggcca ggaggatgcc
     4201 gatgacatcg tcgaggctct cgccaaccac cggatagcgc gagtgcgcag cctcgatgat
     4261 cgagggcaga aattccttgg gcgtctggtt ggccttgatg ctcatcatct gtgagcgcgg
     4321 caccataatg tcgcgaacct gcaggtcggc gacctgaatt gcaccttcga cgatggccag
     4381 tgcttcgctg tcgagcagct tgttctgatg cgcttcgcgc agcacttcca gcaattcctt
     4441 gcggtttttc ggctcatgag caaaagcctg ggtcagtctg ttaaaccagg acttctgctc
     4501 gttgctcgat
//
LOCUS       DASRUM010000054         3214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1869730, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000054 DASRUM010000000
VERSION     DASRUM010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869730"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VFF22_04030"
     CDS             complement(<1..568)
                     /locus_tag="VFF22_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZX16024.1"
                     /translation="MSFTAIQLPIRASSARTLLSGLVLGALLSACASPPPGGVKVVDR
                     THGGASQAPQRQPVSSGQYQVQRGDTLYSIAFRFGWDWKALAARNGIAPPYLIRVGQI
                     IRFDGQPASRPPVVATAPVVVTPSGRPSTPIQTKPVQTQPPAPVKTTPSVTALPPTQR
                     SASGWLWPSNGAVIGRFSSNGSLNKGIDI"
     gene            complement(625..1563)
                     /locus_tag="VFF22_04035"
     CDS             complement(625..1563)
                     /locus_tag="VFF22_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HZX16025.1"
                     /translation="MKKYFLLYAKGIAMGAADVVPGVSGGTVAFITGIYDELLRSIAS
                     VPVALGLFLRGRIVEAWKAANATFLLVLLLGIMTSVLSLARLITYLLDEQPIPVWSFF
                     FGLILVSTHLVVREVQRWNWTRVVSLVLGAAFAYWITVASPLQWGHDPLSLFFAGAIA
                     ICAMILPGISGSFILVLLGLYSFVLTAVKGLDITVLLIFASGCLVGIVSFAGVLRWLL
                     ARWRDLTLAFLTGLMIGSLNKIWPWKETLTWRADSHGEQVPLLQANLWPAQFAEISGQ
                     DPQLLLAIALAVVGILLVLGLEWLAGRNPQSAGDNP"
     gene            complement(1587..2222)
                     /locus_tag="VFF22_04040"
     CDS             complement(1587..2222)
                     /locus_tag="VFF22_04040"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HZX16026.1"
                     /translation="MTSQRTRERLIQRLYEEGLSNAHVLEAIRRTPRHLFVDEALAHR
                     AYEDTALPIGNNQTISQPYMVARMSELLLAAGPLDKVLEIGTGSGYQTAILAQLVERV
                     FSVERIQSLQEKAKERLTQLNLRNVVFRWGDGWEGWNALGPYNGIIVTAAAANVPQAL
                     LDQLAPGGRLVIPVGACDVQELLLIVREEDGFSRHVLDAVRFVPLLNGPLA"
     gene            complement(2264..3013)
                     /gene="surE"
                     /locus_tag="VFF22_04045"
     CDS             complement(2264..3013)
                     /gene="surE"
                     /locus_tag="VFF22_04045"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HZX16027.1"
                     /translation="MRILISNDDGVAAPGLAALHAALADYAECTVIAPDQDKSGASSS
                     LTLDRPLHPTTLPNGYISLNGTPTDCVHLGLNGLLPQVADLVVSGINLGANLGDDVLY
                     SGTVAAALEGRFLARPAFAFSLLSRMPDNLPTAAYFARKLVEAHEQLDLPPRTVLNVN
                     IPNLPLEHIRGIQLTRLGHRARAAAPVKVVNPRGKEGYWISVAGDAEDGGPGTDFHAV
                     MQGYVSVTPLQLDRTFMDGFNSLQPWLEGLL"
     gene            complement(3001..>3214)
                     /locus_tag="VFF22_04050"
     CDS             complement(3001..>3214)
                     /locus_tag="VFF22_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HZX16028.1"
                     /translation="SVLVDWLAEADMTHERRILRLPIGGLSWHYPEPDILQLEFVLPA
                     GCFATVLVRELVDLLPAGQTDNPCVF"
BASE COUNT          712 a         1019 c          953 g          530 t
ORIGIN      
        1 cgatatcaat gcctttattc aaactgccgt ttgaggaaaa acgcccgata accgcaccat
       61 tggaaggcca cagccagccc gatgcagaac gctgcgtggg cggtaatgca gtgacgcttg
      121 gggtggtttt caccggcgct ggtggctggg tttgtacggg cttggtctga atgggtgtac
      181 tcggccgacc ggacggcgtg actacgactg gcgcagtcgc caccaccggc ggacgactgg
      241 caggctggcc atcaaagcga atgatctggc caaccctaat caggtaaggc ggcgcaatgc
      301 cattgcgtgc tgccagtgcc ttccaatccc agccgaagcg aaaggcaatc gagtacagcg
      361 tatcgccacg ttgcacctga tactggccac tactgaccgg ctgacgctgc ggtgcctggc
      421 tggcgccgcc atgggtacgg tcaaccacct tgacgccacc cggcggcgga ctcgcacagg
      481 cgctgagcag cgcaccgagg acaaggccac tcaacagggt acgagcactg ctggcgcgaa
      541 tcggcaattg aatggctgtg aaactcacct gcatctccct cgggtaaaac tgcttacatc
      601 ttgctggcgt gcattgtgcc gcaattatgg gttatcacca gcgctttgcg gattgcgccc
      661 agccagccac tccaggccca gcaccagtag gatgccaaca accgccaggg caatcgccag
      721 aagcaactgc gggtcctggc cgctgatttc agcgaactgc gcaggccaga ggttcgcttg
      781 cagcaacggc acctgctcac catggctatc ggcacgccag gtcagggttt ccttccaggg
      841 ccagatcttg ttcagcgaac cgatcatcaa ccccgtgaga aacgccagcg tcaggtcacg
      901 ccagcgcgcc agcagccaac gcagaacccc ggcaaagctg acaatcccca ccaggcaacc
      961 gctggcaaaa atcagcagca cggtaatatc cagccccttt accgcggtca gcacaaagct
     1021 gtacaaaccc agcaacacca gaataaagct gccggaaatc cccggaagaa tcatcgcgca
     1081 gatggcgata gcacccgcga agaacaggct cagcgggtca tgcccccact gcagcggcga
     1141 cgctacggta atccagtagg cgaacgccgc ccccagcacc agactgacaa cccgcgtcca
     1201 gttccaacgc tggacttcgc gcaccactaa gtgcgtggaa accagaatca aaccgaagaa
     1261 gaacgaccag actggaatcg gctgctcatc cagcaggtag gtgatcagcc gcgcgaggct
     1321 cagcacgctg gtcatgatgc ccagcagcag caccagcaga aacgtcgcat tggcggcctt
     1381 ccaggcttcg acgatacgcc cgcgcagaaa taggcctagc gcaaccggca cgctagcaat
     1441 cgagcgcagc aactcgtcat agataccggt aataaaagcc acggtgccac cggatacgcc
     1501 gggcactacg tcggcagcgc ccatggcaat gcctttggcg tagagcagga aatacttctt
     1561 catgagttca tccgtaaaca atcgagtcag gccagaggcc cgttaagcaa gggtacaaag
     1621 cgcacagcat ccagcacatg ccgggagaag ccgtcttctt cacggacgat cagcagcaat
     1681 tcctgcacat cgcatgcacc gacgggaatc accagacgcc cgcccggagc cagctgatca
     1741 agcaacgcct gcggcacatt ggccgccgct gcggtaacga tgatgccgtt gtacggcccc
     1801 agggcgttcc agccttccca gccgtcaccc cagcgaaaca ccacattgcg cagattgagc
     1861 tgggtcagac gctccttggc tttttcctgc aggctctgga tgcgctcgac gctgaacacc
     1921 cgctccacca gctgcgccag aatcgcggtc tggtagcccg agccggtacc gatctccagc
     1981 accttgtcca gcggcccggc cgccagcagc agctcgctca tgcgcgcgac catgtagggc
     2041 tgcgagatag tctggttgtt gccaatcggc agcgccgtgt cttcataggc ccgatgagcc
     2101 agggcctcat cgacaaatag atgacgtggc gtgcggcgga tggcttccag cacatgggcg
     2161 ttggacaggc cttcttcgta aagacgctgt atcagccgct cgcgggtacg ctgcgaggtc
     2221 atgccgatgc cgcggtggtg cagttcgtcc tgttcacggc ggatcacagc aaaccctcca
     2281 gccagggttg caggctattg aaaccgtcca taaaggtgcg atccagctgc agcggggtga
     2341 ccgagacata accctgcatc actgcatgga aatccgtgcc ggggccgccg tcttccgcat
     2401 caccggccac cgagatccag taaccctctt taccacgcgg gttgaccacc ttcaccggcg
     2461 ccgcagcgcg ggcgcgatgg cccaggcggg tcagctgaat gccgcggata tgctccagcg
     2521 gcagattggg gatattcacg ttgagtacgg tgcgcggcgg taaatcgagt tgctcgtggg
     2581 cttcgaccag cttgcgcgcg aaataggccg cggttggcag attgtccggc atgcgcgaaa
     2641 gcagggaaaa ggcaaacgcc ggacgggcca gaaagcgccc ttcgagtgcc gcagcgacgg
     2701 tgccggaata cagcacgtca tcgccgagat tagcgcccag gttgatgccg gacaccacca
     2761 gatccgccac ttgcggcaac aggccgttga ggcccaggtg cacgcaatcg gtcggcgtcc
     2821 cgttcaggct gatatagcca ttggggagcg tggtcgggtg caggggacgg tcgagggtca
     2881 gcgagctgct cgcgccgctc ttgtcctgat cgggggcaat caccgtgcac tcggcatagt
     2941 cggccagcgc cgcatgcagc gcagcaaggc ctggcgcagc gacgccgtca tcgttggaaa
     3001 tcagaatacg catgggttgt ccgtctgccc tgcaggcaac agatcgacga gctcacgcac
     3061 caagacggtg gcaaagcatc cagccggcag gacgaattcc agttgcagaa tgtcaggctc
     3121 gggataatgc cacgacaggc cgccaatggg gaggcgcagg atacgccgtt cgtgcgtcat
     3181 gtccgcttct gccaaccagt cgaccagaac gctt
//
LOCUS       DASRUM010000055         4579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_195553, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000055 DASRUM010000000
VERSION     DASRUM010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195553"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..88)
                     /locus_tag="VFF22_04055"
     CDS             complement(<1..88)
                     /locus_tag="VFF22_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HZX16029.1"
                     /translation="MSLPPNLGPRNGILSLTIKDKSVLYAAYM"
     gene            complement(125..1111)
                     /locus_tag="VFF22_04060"
     CDS             complement(125..1111)
                     /locus_tag="VFF22_04060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HZX16030.1"
                     /translation="MADAYPWQDSLWQQLAGRTQHAHAYLLHGPAGIGKRALAERLMA
                     RLLCQQPAGLDACGQCKSCHLLAAGTHPDNYILEPEEADKAIKVDQVRELVGFVVQTA
                     QLAGRKVVLVEPAESMNVNAANALLKSLEEPSGNTVLLLISHQPSRLLPTVKSRCVQQ
                     ACPLPSEAMSLAWLAQALPGCSAEEHNELLFLAAGSPLAAVKMHEQGVIAQRAQVVEG
                     VKKLLKQQIAPSQLAESWNAVSLQLLFDWFCQWSQLMLRYQLTRDEDGLGQADMRKVV
                     QYLADKTPQPKVLNMQDWLLAQRQKVLGKANLNRVLLLEALLVQWASLPGPG"
     gene            complement(1104..1736)
                     /gene="tmk"
                     /locus_tag="VFF22_04065"
     CDS             complement(1104..1736)
                     /gene="tmk"
                     /locus_tag="VFF22_04065"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZX16031.1"
                     /translation="MSGLFITLEGPEGAGKSTNRDYLAELLREQGIDVLLTREPGGTP
                     LAERVRELLLAPSDEPMAADTELLLVFAARAQHLAQVIRPALARGAVVLCDRFTDATY
                     AYQGGGRGLSEVRIALLEEFVQGSLRPDLTLVFDLPIEVGLARAAARGRLDRFEQEGR
                     QFFDAVRQTYLRRAAEQPQRYRILDAAQSLTQVQRALDELLPQLLELQRG"
     gene            complement(1746..2798)
                     /gene="mltG"
                     /locus_tag="VFF22_04070"
     CDS             complement(1746..2798)
                     /gene="mltG"
                     /locus_tag="VFF22_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HZX16032.1"
                     /translation="MIRKFLLLLEGGVLLAGLLVAFAAWQQHTALHQPLNLTEEYLVD
                     VPAGATPGGVLNRLEADGILDQAFWLRLYWRFNLRNQPLHSGEYRLMPGATASDMLGQ
                     WRRGEVLQYSLTLVEGWNFRQVRAALARETRLELTLSELSDAELMNKLGLPGQNPEGR
                     FFPDTYSFVRGMSDLELLKQAHARLEQVLAEEWAQRAKDLPYKEPYDALIMASMIEKE
                     TGVPQERGEIAGVFVRRLRIGMRLQTDPTVIYGMGERYNGRITRDDLRQPTAYNTYTI
                     DGMPPTPIALVGREAIHAALNPVAGSSLYFVARGDGSHVFSNTLDQHNRAVREYQLKR
                     RADYRSSPAPVVPNNQ"
     gene            complement(2802..3617)
                     /gene="pabC"
                     /locus_tag="VFF22_04075"
     CDS             complement(2802..3617)
                     /gene="pabC"
                     /locus_tag="VFF22_04075"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016986027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HZX16033.1"
                     /translation="MLSWVDGQPAELLSVTDRGMAYGDGLFETIAVNAGQPVLLERHL
                     TRLAEGCARLKLSVDLPTVRAQLLAFSQQLGEGVAKLILTRGDGQRGYALPQPAQPRL
                     ILQATAKPVYPAAYAEQGVRLFACVTRLAEQPLLAGLKHLNRLEQVLARTEWQDAEHA
                     EGLMLDTSGRVIEGIYSNLLLVSGGVLITADLSRCGVAGVMRAELLVQAAAQGVKCQV
                     RDISYAELLAADEVMLCNSVYGVWPVRALLAHDWPVGPLTRKLQAIARDLLDC"
     gene            complement(3617..>4579)
                     /gene="fabF"
                     /locus_tag="VFF22_04080"
     CDS             complement(3617..>4579)
                     /gene="fabF"
                     /locus_tag="VFF22_04080"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012271164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HZX16034.1"
                     /translation="DANRERIGVAMGSGIGGLTNIENNCKSLIEQGPRRISPFFVPGS
                     IINMISGFLSIHLGLQGPNYAIATACTTGTHCIGMAARNIAYGEADVMVAGGAEMAAC
                     GLGMGGFGAARALSTRNDEPAKASRPWDKDRDGFVLSDGAGALVLEELEHAKARGATI
                     HAELIGFGMSGDAYHMTSPPEDGAGAARCMTNALRDAGVNPEQVQYINAHGTSTSAGD
                     KAEVAAVKSVFGEHAYKLAVSSTKSMTGHLLGAAGAVEAIFSILALRDQVAPPTINLD
                     NPDEGCDLDFVAHQAKAMALDVVLSNSFGFGGTNGSLVFRRFAG"
BASE COUNT          929 a         1525 c         1325 g          800 t
ORIGIN      
        1 gcatgtaagc ggcatagagc acggatttgt ccttgattgt cagcgacaaa attccgttac
       61 gcgggcccaa gttaggaggc agactcatgc ggcattcctg attttattca cttaatggcc
      121 gattctagcc cggtccgggc aagcttgccc actgcaccag caaggcttcg agaagcaaga
      181 cacggttaag gttagccttg ccgagaactt tctgacgctg cgccagcaac caatcctgca
      241 tgttcagcac tttcggctgt ggggttttat ccgccaggta ctgcaccacc ttccgcatat
      301 cagcttgtcc cagcccgtct tcatcgcggg tcagctggta gcgcagcatc agctgcgacc
      361 actggcagaa ccagtcgaac agcaactgca gagataccgc attccagctc tcggccaact
      421 gactgggcgc aatctgctgt ttgagcagct tcttcacccc ctcaaccacc tgcgcgcgct
      481 gagcgataac gccctgctca tgcatcttaa ccgccgccag cggcgagccg gcggcaagga
      541 acagcaactc gttatgttct tcggcgctac agcccggcaa cgcctgcgcc agccatgcca
      601 ggctcatcgc ctcgcttggc aacggacagg cctgctgcac gcagcggctt ttcaccgtcg
      661 gcaacaagcg gctgggctga tggctgatca acagcaacac ggtattgccg gacggctctt
      721 ccaggctttt cagcaaggcg ttggcggcgt tgacgttcat cgactcagcc ggctcgacca
      781 gcaccacttt gcgcccggcc agctgcgcgg tttgcaccac aaagccaacc agctcacgga
      841 cctgatcaac tttgatcgcc ttgtcggcct cttccggttc cagaatgtag ttatctggat
      901 gggtgccagc cgccagcaga tggcaggact tgcactggcc acaggcatcc aggccggcag
      961 gctgctgaca caacagccgc gccatcagcc gctcagccag cgcacgcttg ccgatgccgg
     1021 ccggcccgtg tagcaaatag gcatgggcat gctgcgtgcg acccgccagt tgttgccaaa
     1081 gcgaatcctg ccaggggtaa gcgtcagcca cgctgcagct ccagcagttg cggcagcaat
     1141 tcatccagcg cgcgctgaac ctgagtcagg gattgcgcgg catcgagaat acggtagcgc
     1201 tgcggctgct cggcggcgcg gcgcaggtag gtctggcgca cggcatcgaa gaactgccgg
     1261 ccttcctgct cgaagcgatc cagccgaccg cgcgccgcag cacgggccag gcccacttcg
     1321 atcggcagat cgaacaccag ggtcaggtct gggcgtagcg agccctgaac gaactcctcc
     1381 agcaaggcga tacgcacctc ggagagcccc cggccaccgc cctgataggc gtaggtcgca
     1441 tcggtaaacc gatcacagag caccacagcg ccacgcgcca gtgccggacg gatcacctgc
     1501 gccagatgct gagcacgcgc ggcaaacacc agcagcagct cggtgtcggc cgccatcggc
     1561 tcatcactgg gcgccagcag cagctcacgc acccgctcag ccagcggcgt gccacccggc
     1621 tcacgggtca gcagcacatc gataccctgc tcgcgcaata gctcggccag gtagtcacga
     1681 ttggtgctct tgcctgcgcc ctccgggcct tccagggtga taaacaaacc gctcacaggc
     1741 tgtccttact gattattggg cactactggg gccgggctcg aacggtaatc cgcgcggcgc
     1801 ttgagctgat actcgcgcac ggcccggttg tgctgatcca gggtgttgga aaatacatgg
     1861 ctgccatcgc cacgggcaac gaaatacagg ctgctgccgg ccaccgggtt cagcgccgca
     1921 tggatcgcct cgcgccccac cagggcaatg ggcgtcggcg gcataccgtc gatggtgtaa
     1981 gtgttgtagg ccgtcggctg acgcagatca tcacgggtaa ttcggccgtt gtagcgctcg
     2041 cccataccgt agatcacagt cggatcggtt tgcagacgca tgccgatacg cagacgccgc
     2101 acaaacaccc cggcaatttc gccacgctct tgcggcacgc cggtttcctt ctcgatcatc
     2161 gaggccatga tcagcgcgtc ataaggttct ttgtacggca ggtccttggc tcgctgcgcc
     2221 cattcctcgg ccagcacctg ctccagtcgc gcatgggcct gtttgagcag ctcaagatcg
     2281 ctcatgccgc gcacaaagct gtaggtatcg gggaaaaaac gcccttccgg attctggcct
     2341 ggcaggccga gcttgttcat cagctcggca tcgctcagct cactcagggt cagctcaaga
     2401 cgggtttcac gcgccagtgc cgcacgcacc tggcggaaat tccagccttc aaccagcgtc
     2461 aggctgtatt gcagcacttc accacgtcgc cactggccga gcatatccga tgccgttgcg
     2521 ccgggcatca ggcggtattc gccactgtgc agcggttgat tgcgcaggtt gaagcgccaa
     2581 tacaggcgca gccagaaggc ctgatcgagg atgccatcgg cctcaagacg attgagtacg
     2641 ccacccggcg tcgcgcctgc cggcacatcc accagatact cttcggtcag gttcaagggc
     2701 tggtgcagcg ccgtatgctg ttgccaggcg gcaaacgcaa ccaacaagcc agccagcagc
     2761 acgccgcctt ccagcagcag caaaaatttg cgtatcacga atcagcagtc cagtagatcg
     2821 cgggcaatgg cctgcagttt acgggtgagc gggccgaccg gccagtcgtg tgcgagcaaa
     2881 gcgcgaactg gccacacgcc atagacgctg ttgcacaaca tgacttcgtc cgccgccagc
     2941 aactcggcgt aactgatatc ccgtacctgg cacttgaccc cctgcgcagc ggcctggacc
     3001 agcaactcgg cgcgcatcac accggcaacc ccacaacgcg acaggtcagc ggtaatcaac
     3061 acaccgccac tgaccagcaa caggttgctg taaatgccct cgatcacccg gccactggtg
     3121 tccagcatca gcccttccgc atgctcggcg tcctgccact cggtgcgcgc cagcacctgc
     3181 tccaggcgat tgagatgctt caggcccgcc agtaatggct gctcagccag acgcgtgacg
     3241 caggcaaaca ggcgcacacc ttgttcagca taggctgcgg gataaaccgg tttggcagtt
     3301 gcctgcagaa tcagacgcgg ctgcgcgggc tgtggcagcg cataaccacg ctgcccatca
     3361 ccacgagtga ggatgagctt ggccacgccc tcacccaact gctgactgaa ggccagcagc
     3421 tgcgcgcgca cagtcggcag atcgacggat aacttcagcc gtgcacagcc ttcagccaaa
     3481 cgagtcagat ggcgctctag cagcaccggc tgcccagcat ttacagcgat ggtctcaaac
     3541 agcccatcgc cgtaggccat gccgcgatcc gtgacggaca gcagctcggc cggttggcca
     3601 tccacccagc tcagcatcaa ccggcgaacc ggcggaacac cagggagccg ttggtgccac
     3661 caaaaccgaa ggagttggac agcactacgt ccagcgccat ggccttggcc tggtgcgcaa
     3721 cgaagtccag atcacagcct tcgtccgggt tatccaggtt gatggtgggc ggcgccacct
     3781 gatcacgcag cgcgaggatg ctgaagatcg cctccaccgc accggcagcg ccgagcaagt
     3841 ggccggtcat ggatttggtc gagctgaccg ccagcttgta ggcgtgctca ccaaacaccg
     3901 acttcaccgc agccacttcg gctttgtcac ccgccgaggt cgaggtgcca tgagcgttga
     3961 tgtactgcac ctgctcggga tttacgccag catcgcgcag ggcattggtc atgcaacgcg
     4021 ccgcacccgc gccatcttcc ggtggcgagg tcatatggta ggcgtcaccg ctcatgccaa
     4081 agccgatcag ctcagcgtgg atggtcgcgc cacgcgcctt ggcgtgctcc aactcttcca
     4141 gcaccagcgc gccagcgccg tcggagagaa caaaaccatc acggtccttg tcccaagggc
     4201 ggctggcctt ggccggctcg tcattgcggg tcgacaacgc acgagccgca ccaaagccgc
     4261 ccatacccag gccgcaggcg gccatctcgg cgccaccagc aaccattaca tcggcttcgc
     4321 cgtaggcaat attgcgcgcc gccatgccga tgcagtgagt accggtggta caggccgtgg
     4381 cgatggcgta gttaggcccc tgcagtccca gatgaatcga caggaagccg gaaatcatat
     4441 tgatgataga gccaggcacg aagaacggcg aaatacgccg cggcccctgc tcaatcagcg
     4501 acttgcagtt gttctcgata ttggtcaggc caccgatacc ggagcccatg gccacgccga
     4561 tgcgctcacg gttggcatc
//
LOCUS       DASRUM010000056        27978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1202866, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000056 DASRUM010000000
VERSION     DASRUM010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 27978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27978
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202866"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4420)
                     /locus_tag="VFF22_04085"
     CDS             complement(<1..4420)
                     /locus_tag="VFF22_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZX16035.1"
                     /translation="MWWSKGKSRVTEGAQALASPMIMSLEPRMLFDGAVAATVADAAQ
                     QDAQPAPDNTQTSTAEQASDHQTPQGQTDATEAVVPGKAVLFVDSRVRDAASLLDGVA
                     PGTLVVELDGSKDGLQQIADYLQSHQGISSVQIIAHGNAGDLWLGNSYLSADNIQARS
                     EVLAQIGQNMNAGGDILIYGCYTAEGDRGLSLVESLAQLTGRDVAASDDRTGMGGDWE
                     LEIAAGNIESASVLSASAMSEYEWGLATWTATNNFNAGVGSLRAALTSAQNGDIVTFD
                     IGMTVALTSALVVDKNITIDGDLDNDNVADVTLDGQYRTQILRVTAGTTATLDGLVIT
                     RGMVAGDGGDSGATAQVAQGGGIYNAGTLTLRNVTVTANAASGGGGGGGVSPTYAGGG
                     GGGGGAIATSPGSVGGSGGDSFANGGYFYPGELGSAGEGGDGGNSAGGKGGTAAGVGG
                     AGGTYGGYSSGSAGGTASAGGVSIGGGGGGAGFDAIGGAGGGAVGGIYNDTGATLRVI
                     GNSVISNNIGAGGGGGGGGSYGNNQAGGAGGVGVGAIWNKGSILMTAANFGALAGNVG
                     VGGTGGTSAGTPGSSPAAQNSIYGDGGTLNSNYVPDETPPTATIVVANTSLNSGATSL
                     VTITFSEAVTGFTNADLTIANGTLSTISSSDGGITWTATLTAASNITDTTNLITLNNT
                     GVQDAAGNAGVGTTDSNNYIVNDTVAPTASIVVADTALRIGETSTVTITFSEAVSGFT
                     VADLTVANGTLSGLSSSDGGITWTATLTPDAAITDTSNLIVLNNTGVADLNGNAGVGT
                     TNSNNYAIDTQRPTATIVVSDTALRVGETSVVTITFNEAVSGFTTADLTVANGSVTGL
                     SSSDGGITWTGTLTPSASITDTSNLITLDNTGVADLSGNAGTGTTDSNNYAIDTLRPT
                     ATIVVADTALNIGETSLVTITFSEAVTGFTLADLTVANGSLSGLSSSDGGITWTATLT
                     PSASVSDATNLIILANTGVADAAGNTGSGTTSSNNYAVDTQRPTASIVVADTSLSVGE
                     TSLVTITFNEAVTGFTTADLTVANGTITGLSSGDGGITWTGTLTPSASISDTSNLITL
                     DNTGVADAAGNVGTGTTDSNNYAIDTARPTATIVVADTAVAVGETSLVTITFSEAVTG
                     FTLADLTVVNGSLSGLSTSDNITYTATFTPSAGVSDTTNLITLNNTGVVDGAGNVGSG
                     TTDSNNYAVDTQRPTATIVVADTALSVGETSLVTITFSEAVTGFDNSDLSIANGTLSA
                     VSSSDGGITWTATFTPALGVSDTSNLIVLNNTGVSDGAGNTGTGTTNSNNYQVDTNVP
                     TATIVIADSTLSIGETSLVTVTFNSAVSGFDNSDLTVSNGTLSTMSSTDGGVTWTATF
                     TPSASISDTSNVITLDNTGLINGAGNAGVGTTDSNNYTVDTVRPTATIVVADTAIAAG
                     ETSLVTITFSEAITGFTSADLAVANGVVSGLSSSDGG"
     gene            complement(4471..5307)
                     /locus_tag="VFF22_04090"
     CDS             complement(4471..5307)
                     /locus_tag="VFF22_04090"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016985571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZX16036.1"
                     /translation="MSQFHFISGLPRSGSTLLSAILLQNPRFHAGMTSPVGSLFNGVL
                     QQCSAGSEFGSVIDTDQRRRLLRGLFDSYYADKADKPLIFDTNRQWCARLPALHDLFP
                     QAKTIACVRNVAWVLDSLERIYRANPFENTKLFNDDDERNTVYSRCETLAQRNRLVGF
                     AWTALKEAYYGEHAESLLIVDYDLLSQAPERVMRLVYEFIGEPWFEHDFNRLTYDAPA
                     FDQSLGVAGLHKVRPEVAPQVRRTLLPPDLFEKYAALSFWRDGASSAANVIRMKTDAA
                     IS"
     gene            5619..6206
                     /locus_tag="VFF22_04095"
     CDS             5619..6206
                     /locus_tag="VFF22_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018929399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HZX16037.1"
                     /translation="MEVFIGTIQSFAFNFAPRDWALCNGQTLSISQYQALYALIGVTY
                     GGDGQNTFLLPNLQGRLPLGQGSGPGLTPRVIGTISGTEAVTATLTNLPNHTHALAAI
                     TAATTVELANPASNPVSAPTATNSFLGTSGGGPGTATIYSDQLGASPVPLKGVNTTVS
                     GTVSPAGGGTPMAIMNPFLAINFSIALQGIFPSRN"
     gene            6255..6566
                     /locus_tag="VFF22_04100"
     CDS             6255..6566
                     /locus_tag="VFF22_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007940987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16038.1"
                     /translation="MLQQVHSHHFQPLLGQISNLTLPDGSCLPVRIETLEETPRSKMP
                     HSQRMPFNVALNSLQATAFVDGLCTLEVPELGRLEGLFVSRTPALGRDPALGYFYIAF
                     N"
     gene            complement(6632..7123)
                     /locus_tag="VFF22_04105"
     CDS             complement(6632..7123)
                     /locus_tag="VFF22_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003196579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX16039.1"
                     /translation="MKSVVADGLVVRPAQASDGPFLQNLYQAARPDLQWIDGEPEHIQ
                     QVIAQQFQVQEQGLGEHFPDAMHYVVEKLGTAVGALSTDFGPNEIRVLYLAFIPQARG
                     QGYGRAVLQGVQAAAQQIRCPVATVVWASNPHARQHYLALGFEVEERNPAAERLVWYP
                     KGN"
     gene            complement(7133..9229)
                     /locus_tag="VFF22_04110"
     CDS             complement(7133..9229)
                     /locus_tag="VFF22_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HZX16040.1"
                     /translation="MSLPSLRPDLQLSSAAPDPDGSPQWTLADPVRGRYFKLGAAAMR
                     LLRHWSLGDPQQVLHAANREPGLPLTGESLEQLLVFLRGHDLISSMDPQQRDSYQFKT
                     AAQRQSIWQILLHQYLFFRIPLWRPDAFLNRTWPWLARFGPGILRYGIPLTLGVGIFL
                     VSRDWQRFIATFPHLFSLGGALAFAVALFFAKLCHEFGHAFMAKRAGCRVQSMGVAFM
                     VLLPMFYTDVSDAWRVNDRRARLLIGAGGVLAELLLACVALLAWSLLPDGPARTAAFM
                     LASATWITTLIINLNPFMRFDGYFLLSDFWAVDNLQGRAFALCRWRLREALFGYGAAA
                     PEPWSTTMRRRLLVWGYGAWLWRAALFLGIALAVYHLFFKVLGIFLMVVELVWFIFMP
                     IATEWRQWWSHREQAHGPRVLLTGLGLLALLLALLLPWRNAVEVPAMFEAGRTSTLHT
                     PVAAQIKQVNVQDGQSVAPGDVLIELDSPDMASRLAIVRREIEILQLQMRRQAGRSET
                     AADAGIVEQQLAEAVAEYRGLAAQRERLLLRAPHAGEVRDLLANLTAGRWLSPSQPLA
                     RVVQRDSRLRGYLAEAELWRVEPGAVGRFIADDPMHPSIAVQLDEIDSNGVGWLELQA
                     LASDHHGPIAVRRDSQQRATPVQAQYGVRLSALDEIPAPAQPLRGVVVLQGQGESVLG
                     AAWRRLAALGVRESGF"
     gene            complement(9231..10550)
                     /locus_tag="VFF22_04115"
     CDS             complement(9231..10550)
                     /locus_tag="VFF22_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018929402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZX16041.1"
                     /translation="MNAPLMGNAEQAFARFLDLERQTRAARTPAQLSYSLVNDGQALF
                     GFRHAALLIAGKVEAVTGVSAVEPNAPFVAFVEQAVAQLFKQGVLNQARVIPPDGVSE
                     SIQADWRSLSAGQVFWLPLIDHQSQVFGGLWLARDLPWSPPEQVLLSQLGDTYSHAWL
                     ALQPRKPWRLRWTRKRQAALLAALLLVLLIPVRQSVLAPAEVVPLGGQVVAAPLDGVI
                     AEFLVKPNQSVNNGDLLLRFESTSLKAQADVAERALGVAEAELKANSQRSFADAESSS
                     RIDLLAARVEQKRAEYNYAIELLKRSEVRAERDGIAVFADVERWLGKPVQTGERLMEI
                     ADPTQAELRIELAVGDAIALAPDAPVALFLDSDPLHRHQAWLERSAYEAQPTAAGQLA
                     YRLDARFDAAAPRIGLRGTAKIFGERAPLALYLLRRPLAGLRQSLGL"
     gene            complement(10547..11326)
                     /locus_tag="VFF22_04120"
     CDS             complement(10547..11326)
                     /locus_tag="VFF22_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZX16042.1"
                     /translation="MRYLSGWVVGLVLVASGAQAQTPASDDPLLESSVSSAAPSGAEA
                     RGVLRARDQAVLASELAGRIVELPFSEGESFKKGDILARFDCSAYQAQLKAAQAASRG
                     ANEELAHNRQLAALNSVGRFEVARAEARLSEAQAQSQVYQVQVKRCSVVAPFDGQVVA
                     RKVQRYESVAAGAPLLDVVDNRTLEIHLLVPSRWMSKLKPGQSFTFIPDETGQPLQAT
                     VKRLGARIDEGSQTLLLVATLPDVSGLLSGMSGTAQFAELK"
     gene            complement(11462..12064)
                     /gene="cysC"
                     /locus_tag="VFF22_04125"
     CDS             complement(11462..12064)
                     /gene="cysC"
                     /locus_tag="VFF22_04125"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008028368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HZX16043.1"
                     /translation="MPQAKNLTSVPHRVSADARRHYFGHEGAVVWLTGLSASGKSTLA
                     MGLEEKMLTLGYACYTLDGDNVRNGLNANLGFSPVDRTENIRRVGEVAALFADAGLIC
                     ITAFISPYQKDRLLARRASSRTRFHEIYVSADLSTCEARDPKGLYRKARAGELCDFTG
                     IDAPYEIPDSPDLVINTRHESPAQCIEYLLDYLLKQHPLR"
     gene            complement(12170..13933)
                     /locus_tag="VFF22_04130"
     CDS             complement(12170..13933)
                     /locus_tag="VFF22_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NahK/ErcS family hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HZX16044.1"
                     /translation="MAKPSELSQSQSEALAGLLGLGSQSARKSHYPELLARLEELETE
                     RNRYKWLFEHAVHGIFQASLQDGLLAANPALARMLGYDDPQQVLWSLNGLANYLFIGG
                     VAELEHIRSVLADEPGLFGYETQLRRRDGSVIDVLMNLLLKPGENGLFEGFVADITER
                     KQAQQRLQQLNDELEQRVGARTGELQEANRNLLQQIVERKRIEKALREARDAAEEANR
                     SKDKYLAAASHDLLQPLNAARLLISTLRERRLPASEQNLVERTHQALEGAEDLLTDLL
                     DIARLDQAAIKPDLDVYSLGELLAPLASEFQPVAAAVGLQLRVRHGEHLLNTDLRLLT
                     RILRNFLSNACRYTEQGRILLGARKRGGYVELQVWDSGTGIAEDQLKNIFLEFNQLNV
                     GRAAERKGVGLGLAIVERIARILDYPIKVRSQPGRGSVFSIRVPLASPTQQRAVVALA
                     IPQQRLGDPLPGRRLLVLDNELSILHSMSALLEQWGCQVLTATDQASALEVLGARPPE
                     LILADFHLDHGVVGSEVVQHLRQHFGTNIAAVMITADRSDQCRRELHGMGVPLLNKPV
                     KPGKLRAVLSQILQDLQMPRD"
     gene            complement(13917..15080)
                     /gene="ercA"
                     /locus_tag="VFF22_04135"
     CDS             complement(13917..15080)
                     /gene="ercA"
                     /locus_tag="VFF22_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase-like regulatory protein
                     ErcA"
                     /protein_id="HZX16045.1"
                     /translation="MSHTLSQLRKFVSPEIIFGAGCRHNVANYASTFGARKVLVVSDP
                     GVVAAGWVSDVEVSLQAQNIDYCLYTDVSPNPRVEEVMLGAEIYRANACNVIVAIGGG
                     SPMDCAKGIGIVVAHGRSILEFEGVDTIRVPSPPLILIPTTAGTSADVSQFVIISNQQ
                     ERMKFSIVSKAVVPDVSLIDPETTLSMDPFLSACTGIDALVHAIEAFVSTGSGPLTDT
                     HALEAMRLINGNLVQMIATPNDIVLREKIMLGSMQAGLAFSNAILGAVHAMSHSLGGF
                     LDLPHGMCNAALFEHVVAFNYSAAPERFRMVAQTLGIDVRGLNHNQIRQRLVEHLVAF
                     KKSVGFHETLKLHGVSSSDIPFLSRHAMEDPCILTNPRESSQRDVEVVYGEAL"
     gene            complement(15175..17094)
                     /locus_tag="VFF22_04140"
     CDS             complement(15175..17094)
                     /locus_tag="VFF22_04140"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZX16046.1"
                     /translation="MQDVGCAVRTDPDLIWSAEQAAAASTDFAELHAAHGGVLWVAFD
                     PQQARCGLFFWRDGVSCELTPQGFSVRSRVYEYGGGACCATDQGVAFVNESDQQIYRI
                     DIAEGAGAHSAPYQLSDSPHCRYGDLSFVPAWQALLVVEESHEAGSVVHRLVRISLTG
                     KRDVLVEGADFYAAPIANSCGQRLVWIEWDRPQQPWVATRLCQRDSGERTRVLAGQAG
                     DQSLQQPRFDAQDRLWVLSDQAGWWQPECVDADTKREAAPYDHAPAPWQLGGRTYLPL
                     EHDELLLTRCEEGFAVLLDVGCAVRTNVVCAGVDGAHNLGGPASSYEGAAGELRLAEN
                     FTRFRALAADAGHYYCIAASPSRLPVVLAIARGDGAVRVLAGGESPLNETHLSLPQPV
                     DFAVGEGERCYGFFYAPVEEGEKPPLLIFLHGGPTSACYPVFDPRIAFWTLRGYAVLD
                     LNYRGSSGYGRAYRLRLARQWGELEVEDIRAAIDTLAREGYIDPRRVFVRGGSAGGFS
                     ALRALVELPQLRGGASLYGVSDPLALRQLTHKFEADYLDWLIGDPEQDAERYQCRTPL
                     LQAERITAAVIFFQGALDAVVVPSQTESMVEALRLRGLPVEYHLFAEERHGFRQASNL
                     AAALRAEHAFYQQLS"
     gene            complement(17082..18236)
                     /gene="pqqE"
                     /locus_tag="VFF22_04145"
     CDS             complement(17082..18236)
                     /gene="pqqE"
                     /locus_tag="VFF22_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HZX16047.1"
                     /translation="MHSSGSSFSKPGHEAGPPLWLLAELTYRCPLQCPYCSNPLDFSQ
                     QGAELSTAEWLEVFRQARELGAAQLGFSGGEPLVRQDLAELIKGARDLGYYTNLITSG
                     IGLTEARIAEFAEAGLDHIQISFQAADEEVNNLLAGSKKAFAHKLAMARAVKAHGYPM
                     VLNFVTHRHNIDNIERIIELCLELEADFVELATCQFYGWAELNRAGLLPTRAQLDRAE
                     RITNQWRDKLAAENHPCKLIFVTPDYYEERPKACMNGWGNLFLDITPDGTALPCHSAR
                     QLPVQFPSVREHSIEHIWRHSFGFNRFRGDDWMPEPCRSCDEKHKDFGGCRCQAFMLT
                     GDASNADPVCSKSAQHGVILAARQQADEAPLGLDGLQYRNEKASRIICKM"
     gene            complement(18208..18486)
                     /gene="pqqD"
                     /locus_tag="VFF22_04150"
     CDS             complement(18208..18486)
                     /gene="pqqD"
                     /locus_tag="VFF22_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HZX16048.1"
                     /translation="MTTDILPKVPAIRRGFRLQFEPVQNCHVLLYPEGMIKLNDSAGE
                     ILKQVNGSHSVGQIIAALSERFPDVPGLDEDILAFMEVANAQFWIELQ"
     gene            complement(18572..19324)
                     /gene="pqqC"
                     /locus_tag="VFF22_04155"
     CDS             complement(18572..19324)
                     /gene="pqqC"
                     /locus_tag="VFF22_04155"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HZX16049.1"
                     /translation="MSQTAMSPAEFEQALRAKGAYYHIHHPFHRAMYAGTASREQIQG
                     WVANRFYYQVCIPLKDAAIMANCPDRDTRREWVQRILDHDGAPGEEGGIEAWLRLAEA
                     VGLDREQVLSQELVLPGVRFAVDAYVNFARRASWQEAASSSLTELFAPQIHQSRLDTW
                     PQHYPWIDATGYDYFRKRLKEARRDVEHGLRITLAYYRTVEGQQRMLEILQFKLDVLW
                     SMLDAMSMAYELERPPYHTVTAERVWHRGIAL"
     gene            complement(19353..20267)
                     /gene="pqqB"
                     /locus_tag="VFF22_04160"
     CDS             complement(19353..20267)
                     /gene="pqqB"
                     /locus_tag="VFF22_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HZX16050.1"
                     /translation="MHIQILGSAAGGGFPQWNCNCRNCRGVRTGTLRAQPRTQSSIAI
                     SDDGEQWILCNASPDIRAQIEAFPALQPARKLRDTAIAGIVLLDSQIDHCTGLLTLRE
                     GCPHQVWCTEMVHQDLTSGFPLFNMLSHWNGGLQHRLIELDAEPFSIPACPALNITAI
                     ALRSSAPPYSPHRGNPHPGDNIGLFIEDGRSGRSLFYAPGLGQVDEHLLSWMRRADCL
                     LVDGTLWRDDEMRVCEVGDKLGSEMGHLPQSGPGGMLEVLDGLPASRKILIHINNTNP
                     ILDLDSPERAQLAAHGVDVAWDGMSINL"
     gene            complement(20313..20384)
                     /gene="pqqA"
                     /locus_tag="VFF22_04165"
     CDS             complement(20313..20384)
                     /gene="pqqA"
                     /locus_tag="VFF22_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HZX16051.1"
                     /translation="MWTKPAFTDMRIGFEVTMYFANR"
     gene            complement(20721..22241)
                     /locus_tag="VFF22_04170"
     CDS             complement(20721..22241)
                     /locus_tag="VFF22_04170"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZX16052.1"
                     /translation="MLYAKPGTPGAVITLKPRYGNYINGEFVAPLSGQYFSNTSPVDA
                     SVIGEFPRSDAADIERALDAAHAAADAWGKTSVQNRSLVLLKIADRIEANLEVLAVAE
                     TWDNGKAVRETLNADVPLAADHFRYYAGCIRAQEGSAAEIDEHTAAYHFHEPLGVVGQ
                     IIPWNFPLLMAAWKLAPALAAGNCIVLKPAEQTPLSITVLMELIGDLLPPGVLNVVQG
                     FGREAGEALATSKRIAKIAFTGSTPVGSHILKCAAENIIPSTVELGGKSPNIFFEDIM
                     NAEPAFIEKAAEGLVLAFFNQGEVCTCPSRALVQESIYDAFMVEVMKKIKVIKRGNPL
                     DTETMVGAQASEQQYDKILSYLEIAQQEGAELLTGGAAERLEGDLASGYYIQPTLLKG
                     NNKMRVFQEEIFGPVVGITTFKDEAEALAIANDTEFGLGAGLWTRDINRAYRMGRAIK
                     AGRVWTNCYHLYPAHAAFGGYKKSGVGRETHKMMLDHYQQTKNLLISYDINPLGFF"
     gene            complement(22402..23130)
                     /locus_tag="VFF22_04175"
     CDS             complement(22402..23130)
                     /locus_tag="VFF22_04175"
                     /inference="COORDINATES: protein motif:HMM:NF024892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HZX16053.1"
                     /translation="MTLRLLMLLVLAGPVAAQPWGQAQHAVEQWLQGHSVQAQGLTLD
                     VPAWVEDGAFVAVDLRLQEAQPPLSLSLLRSAEADPRIATLQLQAWEAPLRLSTRVRL
                     PRSQQLIVLARDAAGRVWLAAQWVEVLASSCLAPSPAQQGAVPGPLQAWLEPQGRGLE
                     LRSLLRHPMETGRRPTVDGQLVPRHLPTRFDISGTHGNLLRIEPFEGLAANPYWRLLL
                     PAGHTPLLLRWVDANGREFSRQMP"
     gene            complement(23127..24407)
                     /locus_tag="VFF22_04180"
     CDS             complement(23127..24407)
                     /locus_tag="VFF22_04180"
                     /inference="COORDINATES: protein motif:HMM:NF044548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZX16054.1"
                     /translation="MQGLTRRKLLAGAALLPWLLPGALQASVGRARVLVIGGGFAGAT
                     AARYLKRQAAQLDVVLIEPKSDFHTCPFSNHVLAGLRELDSLRQDYRALQQQQVLHVR
                     DRAREIDLQARQVVLQGGQRLRYDRVFLAPGIDMDWQAIEGYDRNAASRLPHAWQAGA
                     QTALLRRQLQAMDDGGLVIISVPDNPYRCPPGPYERASLIAHYLSQAKPRSKVLILDS
                     KDSFSKQALFQQGWQALYGERIEWVGRAGDGRVVRADAGRRELLCEFGQRHRAAVINL
                     IPPQKAAEIAARSGLVDASGWVPIRPATFQAREASDIYVAGDACIAAPMPKSAYSANA
                     QAKVAVAALLADLAGQEPPSATWRNTCYSALAPGQAVSIAADYGIQKQRLVELPGSLA
                     LSPLQPPAGLRAQEAALADAWYQSICADAWGRLA"
     gene            complement(24583..26358)
                     /locus_tag="VFF22_04185"
     CDS             complement(24583..26358)
                     /locus_tag="VFF22_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HZX16055.1"
                     /translation="MKHAGLRQPYLLSALCAAMLVAAAPAWAVTDQEILDDVNTTDQI
                     VTNGLGLQGQRYSPLATLNTDNIKDLRPIWALSFGGEKQRGQQAQPLIKDGVMYVTAS
                     YSRVFAVDARTGRELWQYDARLPDDIRPCCDVINRGVALYGDLVIFGTLDAKLVALHK
                     DTGKVVWRKNVADHKAGYSITAAPLVVNGKLITGVSGGEFGVVGKIEAYDPKNGELLW
                     TRPTVEGHMGYVYKDGKAVENGISGGEAGKTWPGDLWKTGGAAPWLGGLYDPETNLLL
                     FGTGNPAPWNSHLRPGDNLYSSSRLALNPDDGTIKWHFQTTPHDGWDFDGVNELVSFN
                     YQEGGKTIQAAATADRNGFFYVLDRTNGKFIRGFPFVDKITWAKGLDKNGRPIYDDSN
                     RPGAPGSETKGKSVFSAPAFLGAKNWMPMAYSQDTGLFYVPSNEWGMDIWNEDIAYKK
                     GAAYLGSGFTIKPLNEDYIGVLRAIDPKTGKEVWRHKNYAPLWGGVMTTKGNLVFTGN
                     PEGFLQAFNAKTGEKVWEFQTGSGVLGSPVTWEMDGEQYVSVLSGWGGAVPLWGGEVA
                     KRVKNYNQGGMLWTFKLPKELVAKR"
     gene            26663..27916
                     /locus_tag="VFF22_04190"
     CDS             26663..27916
                     /locus_tag="VFF22_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX16056.1"
                     /translation="MGRTTPLSPLLLSCLLLAGLGNLAQAEPIKIGLNYPRTGNYKDE
                     GLELRRGALLAVDELNRAGGVLGRPLQLLSKNTSSQADKAASNVEQFADQGARMVFGG
                     ATSEEAIAAGQRARERGLLYFATLSYANEVTGQAGHRYLFRESNSAWMSARVLGEYLS
                     WHMPRQRYHYILVDDAWGRSMESALRLATNSTDRARHGRSVLPSGVVRREQYLAALNK
                     AASSEADILVLVLLGENLQRSMRIVHNMGLKQRTQIIVPNLTKSVVQQAGPLVMEGVI
                     GTEAWTWNLPSQDNIAQGQAFVERYIEAYQEYPGSTSASAYSIVRQWADAVQRSQSLD
                     SEQLIRALEDHRYQLLKGEQQWRAFDHQNVQSIYAVRVRKRDDVMLDPLKQHYFDIIH
                     HMAGEQAAPSEDEWQQERGEELQLR"
BASE COUNT         5453 a         8848 c         8506 g         5171 t
ORIGIN      
        1 tgccaccgtc gctgctgctc aggccagaga ctacgccgtt ggcaaccgcc aggtcggcgc
       61 tggtgaaacc tgttatcgcc tcgctgaagg ttatggtcac caaggaggtt tctcccgcag
      121 caatagcggt gtcggccacg actatggtcg ccgttgggcg caccgtgtcg acggtgtagt
      181 tgttggagtc ggtagtaccg acaccggcgt tgcccgcgcc attgatcagg ccggtattgt
      241 ccaacgtgat cacgttgctg gtatcggaaa tactggcgct cggcgtgaag gtggccgtcc
      301 aggtgacgcc gccgtcggtg ctgctcatgg tgctgagcgt gccgttgctg acggtcaggt
      361 ccgaattatc gaacccgctg actgccgaat taaaggtcac tgtgaccaac gaagtctcgc
      421 cgatgctcag cgtgctatcg gcaatcacga tggtcgccgt cggcacgttg gtgtcgacct
      481 ggtagttgtt ggagttggtg gtgccggtgc cggtgttgcc cgccccatcg ctgacgccgg
      541 tgttgttcag gacaatgagg ttggacgtgt cgctgacccc gagggccgga gtaaaggtgg
      601 cggtccaggt gatgccgcca tcactgctac tgaccgcact cagcgtgccg ttggcgatgc
      661 tcaggtccga attgtcgaac cccgtcaccg cttcggagaa ggtgatggtg accagcgacg
      721 tctcgccgac actcagcgcc gtatcggcga ccacgatggt ggcggtcgga cgctgggtgt
      781 cgacggcgta gttgttggag tcggttgtac cgctgccgac gttgcccgca ccatccacca
      841 cgcccgtgtt attgagcgtg atcaggttgg tggtatcgct gacacccgcg cttggcgtga
      901 aggtcgcagt gtaggtgatg ttgtcgctgg tgctgagtcc gctcaggcta ccgttgacaa
      961 ccgtcaggtc cgctagggtg aaacctgtta ccgcctcgct aaaggtaatc gtcaccagcg
     1021 aagtttcgcc cacagcaacg gccgtgtctg cgacgacaat agtcgcggtc ggccgcgcgg
     1081 tgtcgatggc gtagttgttg gaatcggtgg tgcccgtacc gacgttgccc gccgcgtcag
     1141 ccacgccggt gttatccagg gtgatgaggt tgctggtgtc gctgatgctg gcgcttggtg
     1201 taagtgtgcc ggtccaggtg atgccgccgt cgccgctgct taggccggtg atcgtaccat
     1261 tggcgaccgt cagatcggcg gtggtaaagc ctgttaccgc ttcgttgaag gtgatggtca
     1321 ccagcgaggt ttcgccgacg ctcagggagg tgtcggcgac aacgatgcta gcggttggcc
     1381 gctgcgtgtc gaccgcgtag ttgttggaac tggtggtgcc gctgccggtg ttaccggccg
     1441 cgtccgccac accggtattg gccaggatga tcaggttggt ggcatcgctg acgctggcgc
     1501 tcggggtgag cgtcgcggtc caggtgatgc caccgtcgct gctgctcagc ccgctcaggg
     1561 agccattggc aacggtcagg tcggccaagg tgaagccggt caccgcttcg ctgaaggtaa
     1621 tggtgaccag cgaggtttcg ccgatgttca gcgcggtatc ggccaccacg atggtcgcgg
     1681 tgggacgcag cgtgtcgatg gcatagttgt ttgaatcggt ggtgccggtg ccagcgttcc
     1741 cggacagatc ggcaacgccg gtgttatcca gggtgatcag gttgctggtg tcagtgatac
     1801 tggcgctcgg ggtaagcgtc ccggtccagg tgataccgcc gtcgctgctg ctcaacccgg
     1861 tgaccgaacc gttggcaacc gtcaggtcag cggtggtgaa accactcact gcctcgttga
     1921 aggtgatagt caccacggag gtttcaccaa cgcgcagcgc ggtgtcggac accacgatgg
     1981 tggcggtcgg gcgttgcgtg tcgatagcgt agttgttgga gttggtagtc cccacgccag
     2041 cattgccatt gaggtccgcc acgccagtgt tattcaggac aatgagattg gaggtatcgg
     2101 tgatcgccgc atccggtgtc agcgtggccg tccaggtgat gccgccgtcg ctactgctca
     2161 ggccgctgag ggtaccgtta gcgaccgtca ggtcggcgac ggtgaaaccg ctcaccgctt
     2221 cgctgaaggt aatggtgacc gtcgaggtct cgccaatcct gagggcagtg tcagccacca
     2281 cgatggacgc cgtgggcgcc acggtgtcgt tgacgatgta attgttcgag tcggtggtgc
     2341 ccacgccggc attgccggcc gcatcctgca cacccgtgtt attcagggtg atgaggttgg
     2401 tggtgtcggt gatattactg gccgcggtca gggtggcggt ccaagtgatg ccaccgtcgc
     2461 tgctgctgat agtgctgagc gtgccgttgg caatcgtcaa gtcggcgttg gtaaagccgg
     2521 ttacggcttc ggagaaggtg atggtcacca acgaggtggc accggagttc aggctggtat
     2581 tggccactac aatggtggca gtgggcggtg tctcgtctgg tacgtagttg ctgttgaggg
     2641 tgccaccgtc gccgtagatg ctgttctgcg ctgccggaga actacctggg gtgccggcac
     2701 ttgtgccgcc cgtaccgcca acaccgacgt tgccggccag tgcgccgaag ttggcggcag
     2761 tcatcaggat cgagcccttg ttccaaatgg cgccgacacc gacgccgcca gctccgcctg
     2821 cttggttatt gccataagaa ccgccaccgc caccaccacc acccgcaccg atgttgttgg
     2881 aaatcaccga gttgccgata acccgtaggg tcgcgccggt atcgttgtag ataccgccaa
     2941 ctgctccgcc accggccccg ccgatggcgt caaagccagc accgccaccg ccgccgccga
     3001 tggacacccc gccagcggaa gccgtacctc cggctgaacc actggaatag cccccgtagg
     3061 tgccgccagc cccgccaacg ccagcagcgg tgccaccctt gccgccagcg ctgttgccgc
     3121 cgtcgccgcc ttcaccagcc gagccgagtt cgcccgggta gaaatatccg ccattagcaa
     3181 aggaatcgcc gccgctgccg ccgacactgc ctgggctggt ggctattgca ccaccaccac
     3241 caccgccacc gccggcatag gtcggcgaca caccgccacc accaccaccg cctgacgcag
     3301 cgttcgcagt aaccgtgacg tttctcaggg tgagggtgcc ggcgttgtag atgccgccgc
     3361 cttgggctac ctgtgcagtt gcgccactgt cgccaccgtc gcccgcgacc atgccgcggg
     3421 taatcaccag gccatcaaga gtggccgtgg taccggccgt tacgcggagt atctgggtgc
     3481 gatattgccc atccagggtg acgtcggcga cgttgtcgtt atcaaggtcg ccgtcgatgg
     3541 tgatgttttt gtcgaccacc agcgcactgg tcagcgcgac ggtcatgccg atatcgaagg
     3601 tgacaatgtc gccgttctgc gcagaagtca gggcagcacg caaggatccc acccctgcat
     3661 tgaagttgtt ggtcgcggtc caggtagcca gcccccattc atattcgctc atggcgctgg
     3721 cagacagcac gctggcgctt tcaatgttgc cggccgcgat ttccagctcc cagtcgccgc
     3781 ccatccctgt gcggtcatcg gaggccgcta catcacgacc cgtcaactgc gccaaggact
     3841 cgaccaggct caggccgcgg tcgccttcgg cggtgtagca accgtagatc aggatatcgc
     3901 caccggcatt catgttctgg ccgatctgcg ccagtacctc gctgcgggcc tggatgttgt
     3961 cggccgacag atagctgtta cccagccaca aatcaccggc gttaccgtgg gcaataatct
     4021 gcaccgagct gatgccctgg tgggactgca gatagtcggc aatctgctgc aggccgtcct
     4081 tgctgccatc cagctccacc accagggtgc cgggtgcaac gccgtcgagc aggctggcgg
     4141 catccctgac acgggagtcg acaaacagca ctgccttgcc cggcacgacc gcctcggtgg
     4201 cgtcggtctg accttgaggt gtttggtgat ctgaagcctg ctccgccgtc gaggtttggg
     4261 tgttatcggg ggccggttga gcgtcttgct gcgcggcatc ggccacggta gcggccacgg
     4321 caccgtcgaa cagcattcgc ggttccaggg acatgatcat tggcgatgcc agggcctgtg
     4381 cgccctcggt tacccgcgat ttgcctttgc tccaccacat aaggttcacc cgaactcgaa
     4441 cgtctctaac acatcaatga aaaaccgcaa tcaactgatc gcggcgtcgg ttttcatacg
     4501 aataacattg gctgcgctgg aggcgccatc gcgccagaat gacaacgcgg catacttttc
     4561 aaacaggtcc ggcggcaaca gggtacgccg gacttgcggc gcgacctcgg gcctgacctt
     4621 gtgcaggccg gcaaccccta gagactggtc aaaggcaggt gcgtcgtagg tcaaacgatt
     4681 gaagtcgtgc tcgaaccaag gctcgccaat gaactcatac accagtcgca tcactcgctc
     4741 cggggcttgg ctcagcaggt cgtaatcaac gatcagcaac gactcggcat gttcgccgta
     4801 ataggcctct ttcagtgccg tccaggcaaa ccccaccagg cggttacgct gcgccagggt
     4861 ttcgcagcgg ctgtagacgg tgttgcgctc gtcgtcgtca ttgaacagtt tggtgttctc
     4921 gaaagggttg gcacggtaga tccgctccag gctatcgagg acccaggcga cgttgcgcac
     4981 gcaggcgatg gttttggctt gaggaaacag atcatgcagg gcaggcaggc gtgcgcacca
     5041 ctgacggttg gtgtcgaaga tgaggggttt atcggcctta tcagcgtagt aggagtcgaa
     5101 cagcccacgc agcaaacggc gccgctggtc ggtgtcgatc accgagccga actcgctgcc
     5161 ggcgctgcat tgctggagaa cgccgttgaa cagcgaaccg acggggctgg tcatcccggc
     5221 atgaaaacgc ggattctgca gaaggatcgc agaaagcagg gttgaacccg agcgcggcaa
     5281 gccggagata aaatgaaact gtgacatccc tgcaccattg ttgatccatt tgtaggacaa
     5341 agactagtcg agtaagcgca ctacgcccag cagtatttcg atatcaaagg cacgctggac
     5401 tgtgaaaagc agatgaaatg agtgagctcg accagctgaa atgtttaatc gggtcggcaa
     5461 gatttttctg cttctgccgc gcggcagaat gaaatagccc gtctacgcta agtctgcgtg
     5521 accaagccaa gcgctcaaag aacgcgcact aatcgccctt tgtctcttcg cagtcttgtc
     5581 gcgtaccgcc agttcaatag ttaagggatg aaaattctat ggaagtattt atcggcacta
     5641 tccagtcgtt cgcatttaac tttgccccca gggattgggc gctatgcaat ggacaaacgc
     5701 tcagcatctc gcaataccaa gcgctatacg cgctgatcgg ggtgacctac ggaggcgatg
     5761 gccagaacac gtttttgctg cctaacctgc aaggccgtct gccgcttggc cagggttcag
     5821 gcccaggctt gacaccccga gtcatcggca caatttcagg caccgaagcc gtcaccgcca
     5881 cgctcaccaa cctgcccaat cacacacatg cactcgcggc catcacggct gcgaccacag
     5941 tggaactggc aaacccagcc agtaacccgg tgagtgcgcc aacggctacc aactcgtttt
     6001 tgggaacctc ggggggcggt ccagggactg cgactattta ctccgaccag ctaggtgcct
     6061 cacccgtacc gctaaagggt gtcaacacta ccgtcagcgg tacagtttcc cccgctggcg
     6121 gcggtacgcc gatggcgatc atgaatccct tcttggcgat caacttcagt attgccctgc
     6181 aaggcatctt cccgtcacgt aactaactga tgaccggccg gagggctttg gccctccggc
     6241 cgtggagtgt cgacatgctg caacaggttc atagccacca tttccagccg ttgctggggc
     6301 aaatcagcaa cctgacgcta cccgacggca gttgcttgcc cgtgcggatc gaaactctcg
     6361 aagaaacacc tcgctcgaag atgccgcaca gccaacgcat gcctttcaat gtggcgctca
     6421 atagtttgca agccacagcc tttgtggatg ggctctgtac gttggaggtg cccgagctgg
     6481 gccgactgga agggctgttt gtgtcacgca cccccgcttt ggggcgagat ccggcgctgg
     6541 ggtatttcta tatcgcgttt aactaaacga gtatcaagtg acgactgtgg cgaggcacct
     6601 gcgacctcgc cacagtcggt tatgcacaag ctcagttgcc tttcggatac cagaccagcc
     6661 gctccgccgc cgggttgcgt tcttcgacct caaagccaag tgccagataa tgctggcgcg
     6721 cgtgggggtt gctggcccag accacggtcg cgaccgggca acgaatctgc tgggcagcag
     6781 cttgcacgcc ttgcaacact gcgcggccat aaccctggcc acgggcctgg gggataaagg
     6841 ccaggtacaa cacccgaatt tcattaggac caaaatcagt gctcaaggcg cccacagcgg
     6901 tgccgagctt ttccacgacg taatgcatgg catcgggaaa atgctctccc agcccttgct
     6961 cctgcacctg aaattgctgc gcaatcacct gctgtatgtg ctcaggctca ccgtcgatcc
     7021 attgcagatc cggccgtgct gcctggtaaa ggttctgcaa aaatggccca tcgctcgcct
     7081 gcgcaggacg tacgaccaac ccgtctgcta ccactgattt catgtctgct ccctaaaatc
     7141 cgctttccct tacacccagc gccgccagtc tgcgccaagc cgcccccagt accgactctc
     7201 cctggccttg cagcaccacc acaccacgca ggggttgcgc aggggcgggg atctcatcaa
     7261 gtgcactcaa gcgcacacca tattgcgcct gcactggcgt cgcccgttgc tgcgaatccc
     7321 ggcgtacagc aatcgggcca tggtggtcgg aggccaacgc ttgtagctca agccagccca
     7381 cgccgttgct gtcgatttca tccaattgca cagcaatcga cgggtgcatc gggtcatcag
     7441 caataaagcg ccccacggcc cccggttcga ctcgccacag ctcagcttcg gcaaggtagc
     7501 cacgcagccg cgagtcgcgc tgtaccaccc gcgccaacgg ctgcgagggc gacaaccaac
     7561 ggcccgccgt caggtttgcc agcagatcgc gcacctcacc ggcatgaggc gcacgtaaca
     7621 gcaagcgctc tcgctgagcg gccaagcctc ggtattcggc caccgcttca gccaactgtt
     7681 gttcgacgat accggcatcg gctgcggttt cactgcgtcc ggcctgacgg cgcatttgta
     7741 actgcaagat ctcgatttcc ctgcgcacga tagccagacg tgaagccatg tccggggagt
     7801 ccagttcaat cagcacatcc cccggggcta cgctctggcc atcctgcacg ttgacctgtt
     7861 tgatctgcgc tgccacgggg gtatgcaagg tgctggtgcg cccggcctca aacattgctg
     7921 gcacctccac cgcattgcgc cagggcagta ataacgccag taacagcgcc agcaacccca
     7981 gacctgtcag cagtacccgc gggccatggg cctgctcacg gtgactccac cattgccgcc
     8041 actccgtcgc gatgggcata aaaataaacc acaccagttc caccaccatc agaaaaatac
     8101 ccaacacctt gaagaacagg tgatagaccg ccagggcaat gcccaagaac aaggccgcac
     8161 gccacagcca ggccccgtag ccccagacca gtaagcgacg gcgcatcgtt gtcgaccaag
     8221 gctctggtgc cgccgcacca tagccgaaca gggcctcacg caggcgccaa cggcacaggg
     8281 caaaggccct gccttgcagg ttgtccaccg cccagaaatc actgagcagg aaataaccgt
     8341 cgaaacgcat gaacgggttc aggttgatga tcagcgtggt gatccaggtg gcactggcca
     8401 gcatgaacgc tgcagtgcgt gccggcccgt cgggcaacag cgaccaggcc agcaaggcga
     8461 cgcacgccag gagcaattct gccagcacac cgcctgcgcc gatcagcagt cgcgcccgcc
     8521 gatcgttgac cctccaggcg tcgctaacgt cggtataaaa catcggcaac agcaccatga
     8581 acgccacacc catgctttgc acacgacagc cggcgcgttt ggccataaag gcatggccga
     8641 actcgtggca cagcttggca aagaacagcg cgaccgcaaa tgccagtgca ccgcccaggc
     8701 tgaacagatg cggaaaagtc gcgatgaagc gctgccagtc ccgcgacacc agaaaaatac
     8761 ccacacccag ggtcagcggg atgccatagc gcaagatgcc agggccgaac cgcgcgagcc
     8821 acggccaggt gcggttgaga aaagcgtccg gtcgccacag cggaatgcgg aaaaacaggt
     8881 attgatgcag caggatctgc caaatgctct ggcgctgggc cgcagtcttg aactgataac
     8941 tgtcgcgttg ttgcgggtcc atcgaactga tcagatcgtg gccacgtaag aaaaccagca
     9001 attgctccag cgactcgccg gtaagcggca gaccgggctc acggtttgcc gcgtgcagca
     9061 cctgctgcgg atcgcccaag gaccagtggc gcagcaaacg catcgccgcc gcaccgagct
     9121 tgaaataacg cccgcgcacc gggtcggcca gggtccattg gggcgagccg tccgggtctg
     9181 gcgcagctga cgacaattgc agatccggcc gtaggctagg caagctcatg ctacagcccc
     9241 aggctctggc gcagaccggc caatggccgc cgtagcaggt acagcgccaa cggcgcgcgc
     9301 tcgccgaaga ttttcgccgt gccgcgcagg ccgatgcggg gcgccgcggc atcaaagcgc
     9361 gcgtccagcc ggtaggccag ttgcccggcg gcggtgggct gcgcctcata ggcggaacgc
     9421 tcaagccagg cctgatgacg atgcaacgga tcactgtcga gaaacaacgc cactggtgcg
     9481 tcgggcgcca gggcaatcgc atcaccaacg gccagctcaa tgcgcaactc ggcctgagtc
     9541 gggtcggcaa tttccatcaa acgctcaccg gtctgcaccg gcttgccgag ccaacgctcg
     9601 acgtcggcaa acaccgcaat accgtcccgc tcggcacgca cctcactgcg cttgagtaac
     9661 tcgatggcgt agttatattc ggcacgtttc tgctcgacgc gcgctgccag caggtcaatc
     9721 ctcgaactcg attccgcatc ggcaaacgag cgctgggagt tggccttgag ctcggcctcg
     9781 gccacgccca aggctcgctc ggccacatcg gcctgggcct tgaggctggt gctttcaaag
     9841 cgcagtaaca ggtcaccgtt gttgacgctc tggttcggct tgaccagaaa ttcggcaatc
     9901 accccgtcca gcggcgccgc caccacctgg ccgcccagcg gtaccacttc ggccggggcc
     9961 agtaccgact ggcgtaccgg tatcagcaac accagtagca gcgccgccaa aagcgcggcc
    10021 tggcgcttgc gcgtccagcg cagtctccag ggcttgcgcg gttgcagagc cagccaggca
    10081 tggctgtagg tatcgcccag ctgcgacagc agcacctgtt caggagggga ccatggcagg
    10141 tcacgggcca gccacaagcc gccgaatacc tggctctgat gatcgatcag cggcagccag
    10201 aacacctgcc cggccgacag gctccgccaa tccgcctgaa tggattcgct caccccatca
    10261 ggggggatta cgcgagcctg attcagcact ccctgcttga acagttgcgc cacggcctgc
    10321 tcgacaaagg ccacgaacgg cgcgttcggc tccaccgcac tgacgcctgt caccgcctct
    10381 accttgccgg cgatcaacag ggctgcgtga cgaaagccga acagtgcctg gccatcgtta
    10441 accaggctgt agctcaattg cgcgggtgtt cgcgcggccc gtgtctggcg ctccagatcg
    10501 agaaaacggg cgaacgcctg ctcggcgttg cccatcagcg gcgcgttcac ttgagctccg
    10561 cgaactgcgc cgtgccgctc atgccggaca acagaccact gacatccggc agggtcgcta
    10621 ccagcagcag ggtctggctg ccctcatcga tacgcgcgcc caggcgcttg accgtcgcct
    10681 gcaatggctg gccggtttca tcggggataa aggtgaaaga ctgaccgggc ttgagcttgc
    10741 tcatccagcg tgatggcacc agcaggtgga tctccagggt gcggttgtcg actacatcca
    10801 gcagcggcgc accggcagcc acactttcat aacgctggac cttgcgcgcc accacctgac
    10861 catcgaacgg cgcgactacg ctgcagcgct tgacctgcac ctgatacacc tgagactgcg
    10921 cctgggcctc gctgagtcga gcctccgccc gtgcgacttc aaaacgcccg acagaattca
    10981 gcgcggccag ttgccggtta tgcgccaact cttcattggc cccccggctc gccgcctggg
    11041 ccgccttgag ctgagcctgg tacgccgagc agtcgaaccg cgccaggatg tcaccctttt
    11101 tgaaagactc gccctcgctg aatggcaact cgacaatacg cccggccaac tcactggcca
    11161 gcaccgcctg atcccgtgcg cggagcaccc cacgtgcctc ggcgccgctg ggtgccgcgc
    11221 tcgatacact gctttcgagc aacggatcat cgctcgcagg tgtctgcgcc tgagcaccgc
    11281 tcgccactag taccaacccc actacccaac cactcaaata ccgcataacc gttactccct
    11341 gacggatggg cggagtctac gacagcagcg gagagcgtca agggaataac cgcagatgga
    11401 agaaatcgcc tgttttatag ttatcgctgt tagatcacgg agtaactgtc gacatgacag
    11461 atcaacgtaa gggatgctgt ttcaacaaat aatccagtag gtactcaatg cattgagctg
    11521 gcgactcgtg ccgcgtattg ataacaaggt ctggtgaatc cggaatttca tatggcgcat
    11581 caataccagt aaagtcgcac aactcccctg cccgcgcttt acgataaagc cctttgggat
    11641 cacgcgcctc gcaggtggac aaatccgctg atacatagat ttcatgaaac ctcgtccgac
    11701 tactcgccct gcgtgccagc aatcgatctt tttgataagg cgagatgaat gcagtgatac
    11761 aaatcaatcc tgcatcagcg aatagtgccg ctacttcgcc aacccgacgg atattctccg
    11821 tacggtctac tggtgagaaa ccaagattgg cattcaggcc attacggacg ttatcgccat
    11881 ccagggtgta acaggcataa ccaagcgtta gcattttttc ttcaagcccc atagccagtg
    11941 ttgacttacc ggaggctgac aaaccggtta gccatacaac tgccccttcg tgcccaaaat
    12001 aatgacgtcg cgcgtcagca gaaacacgat gagggacaga agtaagattt ttggcttgag
    12061 gcatacaaag taggctctag ttattcaggc ggctttttgt ttccaatgtc gagagcaaac
    12121 gctaaacagc cggctgacgt ttatctgggg aatgaacaac acatagagcc tagtcgcgtg
    12181 gcatctgcag atcttgcagt atctggctca gcactgcacg cagcttgccc ggcttgaccg
    12241 gtttgttgag caagggcacg cccatcccat gtagctcccg gcggcactga tcgctgcggt
    12301 ctgcggtgat catcaccgca gcaatgtttg taccgaagtg ctgacgcagg tgctgcacca
    12361 cctcactacc gaccacgcca tgatccaggt gaaagtctgc caagatcagc tccggcggac
    12421 gggcgccaag tacctctaga gcgctagcct gatcggtagc agtaagcacc tgacaacccc
    12481 attgctccag tagggctgac atgctgtgca aaatgctcag ttcgttatcc agcaccaaca
    12541 gccttcgccc cggtagtgga tcgcccaggc gttgttgcgg tatcgcaagc gccacaacag
    12601 cgcgttgctg ggtggggctg gccagcggta cacgaatact aaacaccgaa ccacgccctg
    12661 gctgagaacg cacctttatg gggtaatcga gaatgcgtgc gatacgctcg acgatggcaa
    12721 ggcccaggcc aacacccttg cgctcggcgg cacggccgac attcagctgg ttgaattcga
    12781 ggaagatgtt tttcagctgg tcttcagcaa tccccgtacc gctgtcccac acctgcagct
    12841 cgacatagcc gccgcgcttg cgcgcgccga ggagaatgcg cccttgctcg gtgtagcggc
    12901 aggcgttgct gagaaagttg cgcaaaatgc gcgtaagcag acgcagatcg gtattgagca
    12961 ggtgctcacc atggcgtacg cgtagttgca gcccgaccgc agccgccacc ggctgaaact
    13021 cagaggccag cggcgcgagc agttcgccga ggctgtagac atccagatcg ggcttgatcg
    13081 ccgcctggtc caggcgggca atatccagca gatctgtaag caggtcttcg gccccttcca
    13141 gcgcttggtg agtacgctcc accaagttct gctcactggc aggcaggcga cgctcacgca
    13201 gggtcgagat cagcaggcgt gcggcgttca atggctgtag caagtcatgg ctcgcagcgg
    13261 ccagatactt atccttacta cggttggcct cttcggcggc atcccgggcc tcgcgaaggg
    13321 ccttttcaat gcgtttacgc tcgacgatct gctgcaacaa gttacggttg gcctcctgca
    13381 gctcaccggt acgtgcgcca acccgctgct ccagttcgtc attgagttgc tgcaagcgtt
    13441 gctgggcctg tttgcgctcg gtgatatcgg cgacaaagcc ttcaaacagg ccgttttcgc
    13501 ccggcttgag aagcagattc atcagcacgt cgatcacact gccgtcgcgt cgacgcagct
    13561 gagtttcata accgaagagc cccggctcat cggccagcac actgcgtata tgctccagtt
    13621 cggcgacacc gccaatgaac aggtagttgg ccaaaccgtt cagagaccag agtacctgct
    13681 gcgggtcgtc gtaacccagc atgcgcgcca aggccgggtt ggccgccagc aagccgtcct
    13741 gcagactggc ctggaaaatc ccgtgtacgg catgctcgaa cagccacttg tagcggttgc
    13801 gctcggtctc cagctcctcc agacgcgcca acagttcggg gtaatggctc ttgcgtgccg
    13861 attgactgcc caacccgagc aggccggcaa gggcctcgct ctgcgactgc gacaactcag
    13921 agggcttcgc catagacaac ctcaacatca cgctgactgg actcgcgtgg gttggtcagg
    13981 atgcatggat cttccatggc atgccgcgag aggaacggaa tatcagagct ggaaacccca
    14041 tgcagcttga gggtttcatg gaagcccacc gacttcttga aagccaccag gtgctccacc
    14101 aggcgctggc ggatctggtt gtgattaagc ccgcgcacgt cgatgcccag ggtctgcgcg
    14161 accatgcgga agcgctccgg cgcggcgctg tagttgaacg ccaccacatg ctcgaacagc
    14221 gccgcgttgc acatgccatg cggcagatcg agaaagccgc cgaggctgtg gctcatggca
    14281 tgcactgcgc caagaatcgc gttggagaac gccagcccgg cctgcatgct gcccagcatg
    14341 atcttctcgc gcaggacgat gtcgttgggg gtggcgatca tctgcaccag gttgccgttg
    14401 atcaggcgca tggcctccag cgcatgggtg tcggttagcg gcccgctgcc ggtggaaacg
    14461 aatgcctcga tcgcatgcac cagcgcatcg ataccggtac aggcgctgag gaacggatcc
    14521 atgctcaggg tggtttccgg gtcgatcagc gacacgtccg gcaccaccgc cttactgacg
    14581 atggagaact tcatccgctc ctgctggttg gagatgatca cgaactgcga tacgtcagca
    14641 gacgtaccgg cagtggtagg aatcaggatc agcggcgggc ttggtacacg aatggtgtcc
    14701 acaccttcga attcgagaat gctacggccg tgggcgacaa caatgccgat gcctttggcg
    14761 caatccatcg ggctgccgcc accgatggca acaatcacgt tgcaggcgtt tgcccggtaa
    14821 atttccgcac cgagcattac ctcttccacc ctcgggttag gcgatacatc ggtgtaaagg
    14881 cagtagtcga tattctgtgc ctgcaggctg acttcaacat cgctcaccca accggcagcg
    14941 accacgccag ggtcggacac caccagcacc ttgcgagcac caaaagtact ggcgtagttg
    15001 gcgacgttgt gtcggcaacc ggcaccgaaa atgatttccg gtgaaacaaa tttacgtaac
    15061 tggctgaggg tgtggctcat acaacggcct gttcttgttg ttatgggata ggtttagcaa
    15121 ggtatacgcc agccagccta ctgcattgct cggtttatgc aatgcgacct gtgttcagct
    15181 caactgctga taaaaggcat gctcggcgcg caatgcggcg gccagattgc tggcctgacg
    15241 aaaaccgtgg cgttcttcgg cgaacaggtg atattccacc ggcaaaccac gcaggcgtaa
    15301 tgcctcgacc atgctctcgg tctggctcgg caccaccacg gcgtccagcg cgccctggaa
    15361 gaaaatcacc gctgccgtga tccgttcggc ctgcaacaga ggtgtacggc actgataacg
    15421 ctcggcatcc tgctcggggt cgccgatcag ccagtccaag taatccgctt cgaacttgtg
    15481 ggtcagctgg cgcagcgcca acgggtcgct gacgccgtag aggctggcgc cgccgcgcaa
    15541 ctgcggcaac tcaaccaatg cccgcagcgc gctgaagccg ccggcactgc caccgcgcac
    15601 aaatacgcgc cgaggatcga tatagccttc gcgagccagc gtgtcgatgg ccgcgcgaat
    15661 atcctccacc tccagctcgc cccactgccg ggccaggcgc aagcgatagg cacggccgta
    15721 gccgctgctg ccacggtagt tgaggtcgag tacggcgtag ccgcgcagcg tccagaaggc
    15781 gatacgcggg tcgaacaccg ggtagcaggc cgaggtcggc ccgccgtgca ggaagatcag
    15841 cagcggcggc ttctcgccct cctctaccgg cgcatagaaa aaaccataac agcgctcacc
    15901 ctcgcccacc gcgaagtcaa caggctgcgg cagagaaagg tgcgtttcgt tcaaggggct
    15961 ctcaccaccg gccaataccc gtaccgcgcc atcacctcgg gcaatcgcca gcacgaccgg
    16021 cagccggctc ggtgaggcgg cgatacagta gtaatgcccg gcatccgccg ccagggcgcg
    16081 aaagcgggtg aagttttcag caagacgcag ttcgccggcc gcgccctcgt aggatgacgc
    16141 ggggccgcct aggttgtgcg caccatcgac gcctgcgcat acgacattgg tgcgcacggc
    16201 gcaccctacg tccagcaaca cggcgaaacc ctcctcgcaa cgcgtcagca gcagctcatc
    16261 atgctcaagc ggcaagtagg ttcggccacc cagttgccag ggcgccggcg cgtgatcgta
    16321 gggcgcggcc tccctcttcg tatcggcatc gacgcactcg ggctgccacc accccgcctg
    16381 gtcgctcaac acccacagcc gatcctgcgc atcgaagcgt ggctgctgca gggattgatc
    16441 acccgcctgc ccggccagca cacgggtacg ctcacccgaa tcacgctgac acaagcgcgt
    16501 ggcgacccag ggttgctgtg gacgatccca ctcgatccag accagacgct gaccgcaaga
    16561 gttggctatg ggggcggcat aaaaatcggc accttcgacc agcacgtcgc gcttgcccgt
    16621 aaggctgatg cgcaccaggc gatgcacgac cgaaccggcc tcgtggctct cctcgacgac
    16681 cagtagtgcc tgccaggcgg gcacgaatga cagatcgcca taacggcagt gaggcgaatc
    16741 gctaagctgg tagggtgcgc tgtgcgcacc agccccctca gcgatatcga tacgatagat
    16801 ttgctggtcg ctttcattga caaaggccac gccctgatcg gtggcacagc aagcaccccc
    16861 gccgtattca tagacccggc tgcgaacgga aaacccctga ggggtcagct cacaacttac
    16921 gccatcacgc cagaagaaca gaccgcagcg cgcctgctgc gggtcaaacg ccacccagag
    16981 cacaccgcca tgggcggcgt gcaactcggc gaagtcggta ctggcggcag cagcctgctc
    17041 ggcgctccag atcaggtcgg ggtcggtgcg cacggcgcac cctacatctt gcatatgatt
    17101 cgtgaggcct tctcgttgcg gtattgcagg ccatccaagc ccagcggtgc ctcatcggcc
    17161 tgctggcggg ctgcgagaat cacgccatgc tgcgccgact tactgcacac cgggtcggcg
    17221 ttgctggcat cgccggtaag cataaaggcc tggcaacggc agccgccaaa atccttgtgc
    17281 ttctcgtcgc agctgcggca tggctcgggc atccagtcgt caccgcgaaa gcggttaaag
    17341 ccgaaggaat ggcgccagat atgctcgatg ctgtgctcac gcacactggg aaactgcacc
    17401 ggcagttgcc gcgcgctatg gcatggcaat gccgtgccgt cgggggtgat gtcgagaaac
    17461 agattgcccc aaccgttcat gcaagccttg gggcgctcct cgtagtaatc cggggtaacg
    17521 aagatcagtt tgcacgggtg gttttcagcg gccagcttgt cgcgccactg gttggtgatg
    17581 cgctcagcgc ggtcgagttg cgcacgggtt ggcagcaacc cggcgcggtt gagttcggcc
    17641 cagccataga actgacaggt ggcaagctcg acgaagtcgg cctccagttc caggcacagc
    17701 tcgatgatgc gttcgatatt gtcgatattg tgccgatggg tgacgaagtt cagcaccatc
    17761 gggtagccat gagccttcac cgcccgggcc atggccagct tgtgggcgaa ggcctttttc
    17821 gagcctgcca gcaggttgtt gacctcctcg tccgcggcct ggaagctgat ctgaatatgg
    17881 tccaaccccg cctcggcgaa ctcggcgata cgcgcctcgg tcaggccgat accggaggtg
    17941 atcaggttgg tgtagtagcc caggtcgcgc gcgcccttga tcagctcggc caggtcctga
    18001 cgcaccaacg gttcgccgcc ggaaaagccc agctgcgccg cgcccagttc gcgggcctgg
    18061 cgaaacacct caagccattc ggcggtggac agttccgcgc cctgctggct gaaatccagc
    18121 ggattggagc agtacgggca ctgcagcggg caacggtagg tcagctcggc cagcagccag
    18181 agcggcggcc ctgcttcatg gcccggctta ctgaagctcg atccagaact gtgcattggc
    18241 tacctccata aaggccagga tgtcttcatc gagccctggc acatcgggaa agcgctcact
    18301 cagcgcggcg atgatctggc cgacgctatg gctgccgttg acctgtttga ggatttcccc
    18361 ggcgctgtcg ttgagcttga tcatgccttc cgggtagagc agcacatggc agttctgcac
    18421 cggttcgaac tgcaggcgaa agccgcggcg gatggcgggc accttgggca ggatgtcggt
    18481 tgtcataatt tcggctccgt tgcggtgcgc acggcgcgcc ctacgcggaa tgctgttgta
    18541 gggtgcgccg tgcgcaccga tggtttgcga ctcatagggc gatcccccgg tgccagacgc
    18601 gctcggccgt caccgtgtgg tacggcgggc gctccagctc gtaggccatg ctcatggcat
    18661 cgagcatgct ccacagcacg tccagcttga actgcaagat ctccagcatg cgctgctggc
    18721 cctcgaccgt gcggtaatag gcaagtgtga tgcgcagacc atgctcgaca tcgcggcgtg
    18781 cctctttcag gcgcttgcga aagtagtcgt agccggtggc atcgatccac gggtagtgct
    18841 gcggccaagt atccaggcgc gactggtgga tctgcggggc gaacagctcg gtcagcgagc
    18901 tgctcgccgc ttcctgccag ctcgcgcggc gagcgaagtt gacgtaggca tcgacggcga
    18961 agcgcacgcc gggcagcacc agctcctgcg acagcacctg ctcgcggtcc aggcccacgg
    19021 cctcagccaa gcgcaaccag gcttcgatgc cgccctcctc gccgggcgcg ccgtcgtgat
    19081 cgaggatgcg ctgcacccat tcgcggcggg tgtcgcggtc cgggcagttg gccatgatcg
    19141 cggcatcctt cagcggaatg cacacctggt aatagaagcg gttggccacc cagccctgga
    19201 tctgttcgcg gctcgccgtg cctgcgtaca tggcccggtg aaaggggtga tggatatggt
    19261 aataagcgcc cttggcgcgc agcgcctgct cgaattcggc ggggctcatg gcggtctggc
    19321 tcatcatggc tctccggtgc gcacggcgca ccctacaaat tgatgctcat accgtcccag
    19381 gcgacgtcga cgccatgggc cgcgagttgc gcgcgctcgg gcgagtccag gtcgagaatc
    19441 gggttggtgt tgttgatatg gataaggatc ttgcggctag ccggcaggcc atcgagcacc
    19501 tcgagcatgc cgccggggcc actctgcggc agatggccca tctcgctgcc gagcttgtcg
    19561 cccacctcgc acacgcgcat ttcgtcgtcg cgccacaggg tgccgtccac cagcaggcaa
    19621 tcggcacggc gcatccagct caacaggtgc tcgtccacct ggccgagacc gggggcgtag
    19681 aacaggctgc gaccgctgcg gccgtcctca atgaacaagc caatgttgtc gcccgggtgc
    19741 gggttgcccc ggtgcggcga atacggcggc gcgctgctgc gcagggcgat ggcggtgatg
    19801 ttcagtgccg ggcaggcggg aatgctgaag ggctcggcat ccagctcgat caggcggtgc
    19861 tgcagaccac cgttccagtg gctaagcatg ttgaacagcg gaaagccgct ggtcagatcc
    19921 tgatgcacca tctcggtaca ccagacctgg tgtgggcagc cttcgcgcag ggtcagcagg
    19981 cccgtgcagt gatcgatctg gctgtcgagc aggacgatgc cggcgatggc cgtgtcgcgc
    20041 agcttgcgcg ccggttgcag agccggaaag gcttcgatct gcgcgcggat gtccggcgag
    20101 gcattgcaca ggatccactg ctcgccgtca tcggaaatgg cgatggacga ttgggtgcga
    20161 ggctgtgccc gcagcgtacc ggtccgcacg ccacggcagt tgcggcagtt gcagttccac
    20221 tgggggaaac cgccgccggc ggcggaaccg agaatctgga tatgcatggg aaattccggg
    20281 tagatgcccc gacagagccg gggcaaggcg gatcaacgat tggcgaagta catggtgact
    20341 tcgaaaccaa tgcgcatatc ggtgaaggcg ggtttagtcc acatggcgcg gtcctcttct
    20401 tatcgacgcc ggcgaattcc ggccccttca ttaagccccc cgaccagcag cggccaaatg
    20461 gtactttggg atgaatttcg gctggcattg gtagcgtttg cagggtgtaa acagacatgc
    20521 agcgcggtaa tccgtagggt taagcaacag acacaacacc cagccgctgg cagcagccaa
    20581 taaaaaacgc ggccgaagcc gcgttgcgat agtgacctgt tgcagcagct atctgcccag
    20641 accacaaaca gatctcgcct accggcgaat cgcaggaagg agcccggcgt actggagcgg
    20701 atacgccggg gttacacgag ttagaagaag cccagcgggt tgatgtcgta gctgatcaag
    20761 aggttcttgg tctgctggta gtggtcgagc atcatcttgt gggtttcgcg gccaacgccg
    20821 gacttcttgt agccaccgaa cgcggcgtgc gccgggtaca ggtggtagca gttggtccat
    20881 acgcgaccag ccttgatcgc ccggcccatg cggtaggcgc ggttgatgtc gcgggtccac
    20941 aggccggcgc ccaggccgaa ctcggtgtcg ttggcaatcg ccagggcttc ggcttcgtcc
    21001 ttgaaggtgg tgatacccac caccgggccg aagatctctt cctgaaacac gcgcatcttg
    21061 ttgttgccct tgagcagggt cggctggatg taataaccgc tggccaggtc accttccaga
    21121 cgctcggccg cgccgccggt gagcagctcg gcaccttcct gctgggcgat ttccaggtag
    21181 ctgaggatct tgtcgtactg ctgctcggag gcctgggcac cgaccatggt ttcggtgtcc
    21241 agcgggttgc cgcgtttgat caccttgatc ttcttcatca cctcgaccat gaaggcgtcg
    21301 tagatcgatt cctgcaccag ggcgcgggat gggcaggtgc acacttcgcc ctggttgaag
    21361 aacgccaata ccaggccttc ggcggctttt tcgataaagg ccggctcggc attcatgatg
    21421 tcttcgaaga agatgttcgg cgacttaccg cccagctcca cggtgctcgg aatgatgttc
    21481 tcggcggcgc acttgagaat gtgcgaaccg accggggtgg aaccggtaaa ggcgatcttg
    21541 gcgatgcgct tgctggtggc cagggcctca ccggcttcgc ggccaaaacc ctgcaccacg
    21601 ttgagcacgc ctggcggcag caggtcgccg atcagctcca tcagcacggt gattgacagc
    21661 ggggtctgct cggccggctt gagcacgatg cagttgccgg cggccagggc cggggcgagt
    21721 ttccaggctg ccatcagcag cgggaagttc cacgggatga tctgcccgac cacgcccagc
    21781 ggctcatgga agtgataggc ggcggtgtgt tcgtcgatct cagcggcgct gccttcctgg
    21841 gcacggatgc agccagcgta gtaacggaaa tggtcggcgg ccagcggcac gtcggcgttc
    21901 agggtttcgc gcacggcctt gccgttgtcc caggtttcgg ccacagccag tacttcgagg
    21961 ttggcttcga tacggtcggc gattttcagc agcaccaggg agcggttctg cacgctggtc
    22021 ttgccccagg catcggcggc ggcatgggcg gcgtccaggg cgcgctcaat atcggcggca
    22081 tcggaacgag ggaactcacc gattaccgag gcatccaccg ggctggtgtt gctgaagtac
    22141 tggccactca gcggcgcgac aaactcacca ttaatgtagt tgccgtagcg cggtttaagg
    22201 gtgatgacgg cgcctggcgt accgggtttg gcgtaaagca tggtgtagac ctctcttgtt
    22261 ggcggagcct gccccctggg acgtggagca ggtcatggtt cgatcttagg aagccacctg
    22321 cacgaccggt atgcgcccat ggcacaaaac ctctcgtcat ttggtagtag agcgcgcccc
    22381 agagccgctc actgccccgt ttcagggcat ctgccggcta aactcgcggc cattggcatc
    22441 cacccagcgt agcaacagcg gtgtatgccc agcaggcagc agcaacctcc agtaagggtt
    22501 ggccgccaaa ccctcgaaag gttcgatacg cagcaggttg ccgtgcgtcc cactgatgtc
    22561 aaagcgtgtc ggtaaatgcc tgggcaccag ctggccatcc acggtcggac gccgcccggt
    22621 ttccatcgga tggcgcaaca ggctgcgcaa ctccagaccg cgcccctgcg gttccaacca
    22681 ggcttgcaac gggccaggca ccgcgccttg ctgagccgga ctgggcgcca gacagctgct
    22741 ggccagcacc tcaacccact gcgcagccag ccacacccgc ccggcagcgt cccgggccag
    22801 aacgatcaat tgctgactgc gcggcaggcg cacacgggtc gacaggcgca gcggtgcttc
    22861 ccaggcctgg agctgcagcg tggcgatgcg tgggtccgct tcggcgctgc gcagcaggct
    22921 gagggacagc ggaggctgag cctcctgcag gcgcaggtcg acggccacga aggcaccgtc
    22981 ctcgacccag gccggcacgt ccagggtcag cccctgagcc tgcacgctgt gcccctgtaa
    23041 ccactgctcc acggcgtgct gcgcctggcc ccagggctgc gcagccacag gcccggccag
    23101 caccagtagc atcagcaggc gcagggtcat gccagacgcc cccaggcatc cgcgcagatg
    23161 ctctggtacc aggcatctgc cagcgccgct tcctgggccc gcagcccggc aggcggctgc
    23221 aacgggctga gcgccaggct gcccggtagc tcaaccaggc gctgcttctg aatgccgtaa
    23281 tccgccgcaa tcgacaccgc ctgccccggc gccagcgcgc tgtaacaggt gttgcgccag
    23341 gttgcacttg gcggctcctg gccggccaga tccgccagca aggccgccac cgcaaccttg
    23401 gcctgagcat tggcgctgta ggccgacttg ggcatgggcg cggcgatgca ggcatccccc
    23461 gccacgtaga tgtcactggc ctcgcgggcc tgaaaggtgg ctgggcgaat cggcacccaa
    23521 ccactggcat cgaccaggcc gctgcgggca gcgatttcgg cggctttctg cggtggaatc
    23581 agattaatca ccgccgcgcg atgacgttga ccgaactcgc agagcagctc gcgtcgcccg
    23641 gcatcggcac gcaccacgcg gccatcgccg gcccgcccga cccactcaat gcgttcgcca
    23701 taaagcgcct gccagccctg ctgaaacagc gcctgcttgg agaaactgtc cttgctgtcg
    23761 aggatcagca ccttgctgcg tggcttggcc tggctcaagt aatgggcgat caggctggcc
    23821 cgctcatagg gccccggcgg gcagcgatag ggattgtccg gcacactgat gatcaccagg
    23881 ccgccatcgt ccatggcctg cagttggcgg cgcagcagag cggtttgcgc gccggcctgc
    23941 caggcatgcg gcagacgact ggcagcattg cggtcgtagc cctcgatggc ctgccagtcc
    24001 atgtcgattc cgggcgcgag aaacacccgg tcatagcgca agcgctggcc accctgcagc
    24061 accacctgcc gagcctgcag gtcaatctcc cgcgcccgat cgcggacatg caacacctgt
    24121 tgctgctgca aggcgcggta atcctgacgc aggctgtcca gctcacgcag cccggccagc
    24181 acatgattac tgaacgggca ggtgtggaag tcagacttcg gctcaatcag caccacgtct
    24241 agctgcgcgg cctgacgttt caggtagcgc gccgctgtag caccggcaaa accgccgccg
    24301 attaccagca cacgcgcccg gccgacgctg gcctgcagcg cccctggcaa tagccagggc
    24361 agcaaggctg ccccagcgag caatttacga cgcgtaaggc cctgcatgct tccccctgca
    24421 ccgaaactct gtgcccgaat aaaatcgcaa cagagcctat ctacacgctg aagcacagcc
    24481 gtagagcaga ccatgcttct ttggtcgcaa gtcattgccc ccaacgcatg aaaacaaaaa
    24541 tggccggtct agaccggcca ttctgtacac gctatccgcc aatcaacgct tggcgaccag
    24601 ttccttgggc agtttgaagg tccagagcat gccgccctgg ttgtagttct tcacccgctt
    24661 ggccacttcg ccaccccaca gcggtactgc accgccccag ccggagagca ccgaaacata
    24721 ctgctcgccg tccatttccc aagtcacagg cgaacccagc acaccggagc cagtctggaa
    24781 ttcccagacc ttctcgccgg tcttggcgtt gaaggcctgc aggaaacctt ccgggttgcc
    24841 ggtgaacacc aggttgccct tggtggtcat caccccgccc cacagcggcg cgtagttctt
    24901 gtgccgccag acttccttgc cggtcttcgg gtcaatggcg cgcagcacgc cgatatagtc
    24961 ttcgttcagc ggcttgatag tgaagcccga acccaggtag gccgcgccct tcttgtaggc
    25021 gatgtcctcg ttccagatgt ccatgcccca ctcgttgctc ggcacataga acagcccagt
    25081 gtcctggctg taggccatcg gcatccagtt tttcgcaccg aggaaggccg gcgccgagaa
    25141 cacgctcttg cccttggttt cgctgcccgg tgcacccgga cggttgctgt cgtcgtagat
    25201 cggccgacca ttcttgtcca ggcccttggc ccaggtgatc ttgtcgacaa acgggaagcc
    25261 acggatgaac ttaccgttgg tgcgatcaag aacgtagaag aagccgttac ggtcagcggt
    25321 ggcggccgcc tggatggtct tgccaccttc ctggtagttg aaggacacca gctcattaac
    25381 accgtcaaag tcccagccgt catgcggggt agtctggaag tgccacttga tggtgccgtc
    25441 gtccgggttc agggccaggc gcgaggacga gtagagattg tcgccagggc gcaggtgcga
    25501 gttccacggc gccgggttgc cggtgccgaa cagcagcagg ttggtttccg ggtcgtacag
    25561 gccgcccaac caaggcgccg caccgccggt cttccacaga tcgccgggcc aagtcttgcc
    25621 ggcttcgccg ccgctgatgc cgttctcgac agccttaccg tccttgtaga cgtagcccat
    25681 atggccttcc acggtaggac gggtccacaa cagctcgccg ttcttcggat cataggcttc
    25741 gatcttgccg accacgccga actcaccacc ggataccccg gtgatcagct tgccgttaac
    25801 caccagcggt gccgccgtga tcgagtaacc ggctttatga tcggcaacat tcttgcgcca
    25861 caccaccttg ccggtgtcct tgtgcagggc caccagcttg gcgtccaggg tgccgaagat
    25921 caccaggtcg ccatacagcg ccacgccacg gttgatcacg tcgcagcagg ggcggatgtc
    25981 atccggcaga cgcgcatcgt attgccacag ctcgcgcccg gtgcgggcat cgacagcaaa
    26041 cactcgcgag taggacgcgg tgacatacat caccccatcc ttgatcagcg gctgggcctg
    26101 ctgaccacgc tgcttctcgc caccgaagga cagtgcccag atcgggcgca ggtctttgat
    26161 gttgtcggta ttgagggttg ccagcgggct gtagcgctgg ccttgcagcc cgaggccgtt
    26221 ggtgacgatc tgatcggtgg tgttcacatc atcaagaatt tcctgatcag tgacagccca
    26281 ggccggtgca gcagccacaa gcatggcggc acataaggca ctgagaaggt aaggttggcg
    26341 aagaccggcg tgtttcattg cggctacctc tgcgggttca ttgttatcac cgggaatttt
    26401 tcccgatcta ccgcagattc ttggctgctc aggcctggcc aacaattgca cgcagtgccg
    26461 atttagctac ttccttggta gcaataccgc ccgctttact ccttgcctgc cacacctgct
    26521 gcgtaggcaa atttcccgac ccggccccga ctgatccagg gccaggccga cttaagtgct
    26581 acgcctgccc cctgaaaaga gcatacccag ccctaggccc agccgttaga aagctactcc
    26641 cccacgctaa ggagcaagcc acatgggccg caccacccca ctcagccccc tgctgttgag
    26701 ctgcctgcta ctggcaggcc tgggcaacct tgcccaggcc gagccaatca agatcggcct
    26761 caactaccca cgtaccggca attacaagga cgaaggcctg gaactgcgcc gtggcgcatt
    26821 gctggccgtg gacgagctca accgtgccgg cggcgtgctc ggcaggccac tgcagctgct
    26881 cagcaagaac accagctcgc aggccgacaa ggccgccagc aatgtcgagc agtttgccga
    26941 ccagggcgca cgtatggtgt ttggcggcgc caccagcgag gaagcgatag ccgccgggca
    27001 gcgcgcccgc gagcgcggcc tgctgtactt cgccaccctg agctatgcca atgaagtgac
    27061 gggccaggcc ggccatcgct acctgttccg tgagtccaac agtgcctgga tgagtgcgcg
    27121 ggtgctgggc gaatacctgt cctggcatat gccgcgccag cgctaccact acatcctggt
    27181 tgacgacgcc tggggccgca gcatggaaag cgccctgcgc ctggccacca acagcaccga
    27241 ccgcgcacgc catggccgca gcgtactgcc cagtggcgtg gtacgtcgcg agcagtacct
    27301 ggcggcgctc aataaagcgg ccagcagcga ggcggatatc ctcgttctgg tgttgctcgg
    27361 cgagaacctg caacgcagca tgcgcatcgt gcacaacatg ggcctcaagc aacgtacgca
    27421 gatcatcgta cccaacctga ccaagagcgt ggtgcagcag gccggcccat tggtcatgga
    27481 gggcgtgatc ggcaccgaag cctggacctg gaacctgccc agccaggaca acatcgccca
    27541 gggccaagcg tttgttgagc gctacatcga ggcctatcag gagtaccccg gcagcaccag
    27601 cgcttccgcc tattccatcg tcaggcaatg ggccgatgcc gtgcagcgca gccagagcct
    27661 ggacagtgag caactgatcc gcgccctgga ggaccaccgc tatcaactgc tcaagggcga
    27721 gcagcaatgg cgcgccttcg atcaccagaa tgtgcagagc atctatgcag taagggtacg
    27781 caagcgtgac gacgtgatgc tcgacccgct caagcagcac tatttcgaca tcatccacca
    27841 catggctggt gagcaggccg cgccgagcga ggatgagtgg cagcaggagc gtggcgagga
    27901 attgcagctg cgctagaggc gcattggacg cggctcttgt agggcgggtg aaacccgcgg
    27961 ccagacgctg gcgggttg
//
LOCUS       DASRUM010000057        14276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1203027, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000057 DASRUM010000000
VERSION     DASRUM010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 14276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14276
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203027"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VFF22_04195"
     CDS             <1..335
                     /locus_tag="VFF22_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HZX16057.1"
                     /translation="GFYDGVAGPGTGAFWTVSSLLLYPLNLVKASGVARSMNFVSNIC
                     ALTVFIISGQVAWLLGICMGLALMVGAFLGARTAIKGGSRFIRPVFILVVLALTVRLA
                     WQHWFGLA"
     gene            complement(303..1130)
                     /gene="nudC"
                     /locus_tag="VFF22_04200"
     CDS             complement(303..1130)
                     /gene="nudC"
                     /locus_tag="VFF22_04200"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HZX16058.1"
                     /translation="MQRWQATLLDSQQPGGWVVAHCKQHFMADSNGVLFPREWLKKQD
                     LPIIAEHGLGHFDGDAVYLLELSQPFDMPGCAWQSLRQFMLQGDADTFKLLSYATQIG
                     TWASQHRFCGSCGGAMQHVPGERAMHCVRCELQHYPRLSPSMIVLVTRGDEILLARSP
                     RFVAGVYSTLAGFVEAGESVEHCVAREVREEVGVEISNLQYLGSQGWPFPHSLMLGFH
                     AEYASGDIVMQADEIEDAQWFSVHDLPPLPAARSIARYLIDVYVARRLGQPEPVLPG"
     gene            complement(1133..2812)
                     /locus_tag="VFF22_04205"
     CDS             complement(1133..2812)
                     /locus_tag="VFF22_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016561779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HZX16059.1"
                     /translation="MVSGATSLNLVRDELFTTMEEAESSLEHFIADRHNGSLLQQAVE
                     NLHQVRGTLNLIELAGAELLAQEVLDQATDIPAGAGEERDVQLSALSNALHVLRRYLE
                     NVEAHRQEMPELLLPAINDLRQAGGQPALPESFFFSVRLDHARPRTSPPSVDGAARES
                     EGRRLRHMYQVGLLGFIREQNPQASLKLMGRALTRLDSLFANEPRGRLCWVGSAAVEA
                     QVDGQLLARKSRKQLFSRIDRELKQMLVNGQYEAPRGLLKELLYLVALADSRGPQAAA
                     LGEVFGLTPLPFTDHLLEEEYQRLAGPGQAVMRSLSSAIREELNSVKDMLDLIERGTL
                     QSDSLNSLHALLGKLSKTLGMVGLSSAGNSLSAQLPVVASWSEESAPAPLELHKLADA
                     VLYVEGMVASLERGERRDTRPTQAQPGEEADSFALHQLNEARIVVVDEAQAGLALAKR
                     AITAYLESSGERMHLSNVPFSLQAVRGGLWFLGQERAALLVGACADYIQQHMFDAPQM
                     PSEQMLETLADALSSLEYYLEAGAVMRPETQPSVLDLAAESVRALGMPLEA"
     gene            complement(2863..3675)
                     /locus_tag="VFF22_04210"
     CDS             complement(2863..3675)
                     /locus_tag="VFF22_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZX16060.1"
                     /translation="MSDYKAFNVVLADKVAHVVINRPEKVNAMNADFWREIIEIFRWA
                     DDTDEVRAVVISGAGKHFSSGIDLMMLASVGSQLGPDVGRNAEKLRRKILELQASFNA
                     VDNCRKPVLAAIQGYCLGGAIDLISACDMRYSTADAQFSIKEIDIGMAADVGTLQRLP
                     RIIGDGMMRELAFTGRTIDGEEARSIGLVNRTYADADALLDGVMGIAREIASKSPVAI
                     RGTKEMLRYMRDHRVDDGLEYIATWNAAMLQSADLRVAMTAHMSKQKPEFAD"
     gene            complement(3916..6174)
                     /locus_tag="VFF22_04215"
     CDS             complement(3916..6174)
                     /locus_tag="VFF22_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HZX16061.1"
                     /translation="MYKDLKFPVLIVHRDIKADTVAGDRVRAIAQELEQDGFSILSTA
                     SSAEGRIVASTHHGLACILVAAEGAGENKNLLQDMVELIRIARRRAPQLPIFALGEQV
                     TIENAPAEAMVDLNQLRGILYLFEDTVPFLARQVARAARNYLDCLLPPFFKALVQHTA
                     QSNYSWHTPGHGGGVAYRKSPVGQAFHQFFGENTLRSDLSVSVPELGSLLDHTGPLAE
                     AEARAARNFGADHTYFVINGTSTANKIVWHSMVGRDDLVLVDRNCHKSILHSIIMTGA
                     IPLYLCPERNELGIIGPIPLSEFSRESIQAKIDASPLARGRVPSASGAMVKLVVVTNS
                     TYDGLCYNAELIKQTLGNSVEVLHFDEAWYAYAAFHEFYSGRYGMGTARTDDAPLVFT
                     THSTHKLLAAFSQASMIHVQDGGARQLDRDRFNEAFMMHISTSPQYGIIASLDVASAM
                     MEGPAGRSLIQETFDEALSFRRALANLRQNLSSDDWWFSIWQPAKAEGTDDVVTQDWL
                     LQPQADWHGFGDVAEDYVLLDPIKVTLVTPGLTADGKLDARGIPAAVVSKFLWERGLV
                     VEKTGLYSFLVLFSMGITKGKWSTLLTELLEFKRSYDANLPLSDVLPSIAQAGKTFYQ
                     GMGLRDLCDALHACYRENATAKALKRMYTVLPEVAIKPADAYNQLVRGEVEAVPIELL
                     EGRIAAVMLVPYPPGIPLIMPGERFTAQTRSIIDYLAFARTFDRSFPGFDADVHGLQR
                     EEGVYTVDCIKA"
     gene            complement(6304..7047)
                     /gene="dnaQ"
                     /locus_tag="VFF22_04220"
     CDS             complement(6304..7047)
                     /gene="dnaQ"
                     /locus_tag="VFF22_04220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HZX16062.1"
                     /translation="MRSVVLDTETTGMPVADGHRIIEIGCVELIGRRLTGRHFHVYLQ
                     PDREIDEGAIAVHGITNDALKDKPRFKEVADEFFEFIKGAQLIIHNAAFDVGFINNEF
                     ALIKQDDRADISEHCSILDTLMMARERHPGQRNNLDALCKRYGVDNSGRDLHGALLDA
                     EILADVYLTMTGGQTNLSLAGDGSEGDSNGRQQPSAIRRLPAERARTRVIQASAAELA
                     EHAARLAIIEKSAGAPPLWVQMEQPAAEL"
     gene            complement(7040..7492)
                     /gene="rnhA"
                     /locus_tag="VFF22_04225"
     CDS             complement(7040..7492)
                     /gene="rnhA"
                     /locus_tag="VFF22_04225"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019828395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HZX16063.1"
                     /translation="MSEQVEIYTDGACKGNPGVGGWGALLVCKGVEKELWGGEPNTTN
                     NRMELMAAICALIALTRPCSVRLITDSQYVMKGIQEWMPNWKKRGWKTAAKEPVKNAD
                     LWQALDEQVNRHEVTWQWVRGHTGHPGNERADQLANRGVDEVRGLKHA"
     gene            complement(7511..8290)
                     /locus_tag="VFF22_04230"
     CDS             complement(7511..8290)
                     /locus_tag="VFF22_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZX16064.1"
                     /translation="MSDHPFALADSEWLDLIAAARSWLSGPLGQLLLEQERQLLEEEL
                     ARFFGGYLVHYGPAADTPPDAQQIQRSVRLGAPFKGVEIVCEEHSWPLTEHAADVVVL
                     QHGLDFSLSPHGLLREAARSVRPGGHLLIVGINPMSSWGLRHLFTRDALRQARCIAPN
                     RVSDWLHLLGFALEKRRFGCYRPPLSAPAWQTRLAGLERLGAAWQPPGGGFYVLVARK
                     LVVGLRPLRQPSRQAVGKLIPMPVAKVSRRETPDEQHRPIN"
     gene            8387..9160
                     /gene="gloB"
                     /locus_tag="VFF22_04235"
     CDS             8387..9160
                     /gene="gloB"
                     /locus_tag="VFF22_04235"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HZX16065.1"
                     /translation="MIKIDALPAFTDNYIWLLQDAKQRRCAVVDPGDAAPVEAWLAAN
                     PGWQLSDILITHHHFDHVGGVEKLKTATGARVLGPANEKIPGQDLALRDGDQADVLGQ
                     RFDIYEVPGHTLGHIAYIQPRQHWLFCGDTLFAGGCGRLFEGSPEQMYNSLDRLAALP
                     EQTQVFCTHEYTLSNLRFAAAVEPDNAEIQQRLAQVSQWREAGQISLPSSIGLERATN
                     PFLRSAQASVGARINEREGPASRSPAQVFAALRAWKDHF"
     gene            9259..10836
                     /locus_tag="VFF22_04240"
     CDS             9259..10836
                     /locus_tag="VFF22_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZX16066.1"
                     /translation="MPLSPRKTLNLKALAQSAQTLVVIMCVTLAGCQTSNQYRPDSGR
                     DTDRAVGLEQEPEWLDSSVVAPPEAPKDIWERVRNGYQLQDTITLNPRIEQQRLWFVS
                     NPSFIEKAGERSSPYIHFIVERLEQRNMPMELALLPMIESSYNPLAYSHAHAVGLWQF
                     IPSTGRNFNLRQTNWYDGRRDVMASTDAAISYLTRLKEMFNGDWLLALAAYNAGEGRV
                     SRAIERNQKLGLPTDYWNLTLPVETQNYVPKLLALSQVVMTPQAYGVSLAPIANEPYF
                     EKVVFTQRMDLARVAAMADLDEDELYLLNPAFKKGVTLDGPQHLLVPTDKAELLTANL
                     SLMKPQALVDWQQYRVRSGDSLHSIANRHQLTVNTLKDINKLSGNNLRIGQMLSIPAQ
                     PGVPAREPMFQRSVAHSAPSRTYRVRSGDNLWQIAKNHKVAVKDVQRWNKLSGNSLRV
                     GQVLTLQAGKPSSGGSGSSGNSSRQSATYYKVRQGDSLYLIAKRFKVPMKSLQNWNPK
                     AGKALKPGQTLTLYSVN"
     gene            complement(10837..12282)
                     /locus_tag="VFF22_04245"
     CDS             complement(10837..12282)
                     /locus_tag="VFF22_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HZX16067.1"
                     /translation="MATACHGLQSWIDRFTQAELPALSAVVQDLHRLTQGDKSSVQQL
                     ADVLLRDAALTTKVLRIGNSVYYNPSQEPIRTISRAIVLIGFDNVRQIGLSVSLIDGL
                     LARSSRDQLAELLARSFHAAVQARNIAGYVLSKNDEVVFIAALLHNVGELAFWGCAGD
                     DADDLASALAQPGADEDEVVMRMLGTSFRQLSQALVKSWNLGDTVSLAHQSTSQNDPA
                     VKAVTLGAKISQAALDGWDTPVMEALVGQLASFIGVTPQEAMQQVLASAEETVKMATT
                     FGASQLCKLIPNTDPEQIRLQQEQRKARLLQPNLLILQQALQDLGLLVSRRGDLGLVL
                     DTLFKGLHQGTGLERIMLVVLADGQSCFRAKRVIGEGTEGWSRDFVLPVAQQEQPHIF
                     SYALRNKEALWMGVPASYNLNELVTQPIRQRLGQGMFFIAPLLAGTREIGLLYADSRV
                     SGRALKHEQFVAFQRFTQLTGRCLEAMSKKA"
     gene            12429..14258
                     /locus_tag="VFF22_04250"
     CDS             12429..14258
                     /locus_tag="VFF22_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZX16068.1"
                     /translation="MRPLVSLLLCMAISSSAWATLYKSHGYAQFGELKYPASFTHFAW
                     VNPEAPKGGTLRMMANGTFDTLNPYTLKGSSPISSANFLQYGVNELNAPLMVGTGAYD
                     PSGDEPASSYGLIAESVEYSDDRSWVVFNLRPEARFHDGKPITAYDVAFSYRLLLKEG
                     HPQYRTNLQEVKRVDILNRHSIRFVLKRAGNSLLILRLGELPVLPQHYWKDRDFKATT
                     YEPPLGSGPYRITQVSPGRSLRFERVKDWWGAKLPVNRGKYNFDRVDVEFYRDSHVAF
                     EAFKAGEFDIYIEQQAKNWANGYRFPALSRGEVIRAEIPHQIPTQTQALFMNTRREVF
                     ADRQVREALGLMFDFEWANRALFNSSYTRAKSYYPNSEFSARDKPEGAEWLLLSPYRK
                     QLQARLFSEPFSMPQTDGRGIPRETLRRALGLLAEAGWKPSGQRLLNSDGQPLRFEIL
                     LVNPNLERILQPFTENLASIGIQANLRTVDRAQYKQRLDQYDFDMILLTLPQTLSPGL
                     EQSLYFHSSQAKIKGGRNYAGVTHPVVDALIEKLLSAQSRDEQIAATRALDRVLLWQH
                     YSIPNWYIDYHRLAYRNRFAFVTTPPYTLGLRTWWLKSMENAE"
BASE COUNT         2995 a         4554 c         4056 g         2671 t
ORIGIN      
        1 tggggttcta cgatggagtg gcgggcccag gcactggcgc cttctggacg gtcagcagcc
       61 tgctgctcta cccgctcaat ctggtcaagg ccagcggcgt ggcccgcagc atgaacttcg
      121 tcagcaatat ctgcgcgctg acggtgttta ttatcagcgg gcaagtggcc tggctgctgg
      181 gcatctgcat gggcttggcg ttgatggtcg gggcgtttct cggcgcacgc acggcgatca
      241 agggcggctc gaggtttatc cgcccggtat ttatcctggt ggtgctggcg ttgaccgtac
      301 gcctagcctg gcagcactgg ttcgggctgg cctaagcggc gcgccacata gacgtcgatc
      361 aggtagcggg caatcgagcg tgccgccggt aatggcggca ggtcatgcac gctaaaccac
      421 tgggcatcct cgatttcatc ggcttgcatc acgatatcgc ccgaagcgta ttcagcatgg
      481 aagcccagca tcaatgaatg cgggaacggc cagccctggc tacccaggta ctgcaggttg
      541 ctgatctcaa ccccaacctc ttcgcgtact tcacgggcca cgcagtgctc caccgactcc
      601 cccgcctcca cgaagccggc cagggtgcta tagaccccgg caacgaaacg cggcgagcgc
      661 gccagtagaa tctcatcacc gcgggttacc agcacaatca tgctcggtga aagccgcggg
      721 taatgctgca actcgcagcg cacacagtgc atcgcgcgtt cacccggcac atgttgcatc
      781 gccccaccac agctgccaca gaaccgatgc tggctggccc aggtgccaat ctgtgtggcg
      841 tagctgagca acttgaaggt atcggcatcg ccctgcagca taaactggcg caggctctgc
      901 caggcgcagc ccggcatgtc gaagggctgg ctcagttcca gcaggtagac cgcatcacca
      961 tcaaaatggc caaggccatg ctcggcgata atcggcaaat cctgcttctt caaccattca
     1021 cggggaaaca gcacaccgtt gctatccgcc ataaagtgct gcttgcagtg cgcgaccacc
     1081 caaccaccgg gttgctgact gtcgagcaga gtggcttgcc agcgctgcat gctcaagcct
     1141 ccagcggcat gcccagggct cgcacgcttt ctgcggcgag atcaagcacg cttggctggg
     1201 tttccggacg catcaccgca ccggcttcga ggtagtactc aaggctactg agggcatcgg
     1261 caagggtttc cagcatctgc tcggacggca tctgcggcgc atcgaacata tgctgctgga
     1321 tgtaatcggc gcaggcgccg accaacaacg cggcacgctc ctgaccgagg aaccacaacc
     1381 cgccgcgcac ggcctgcagg ctgaacggca cgttggacag gtgcatgcgc tcgccacttg
     1441 actccagata cgcagtaatc gctcgcttgg ccaaggccag accagcctgg gcctcatcga
     1501 ccaccacaat gcgtgcttca ttaagttgat gcagagcgaa ggagtcagcc tcttcaccag
     1561 gctgcgcctg agtaggccgc gtgtcacgac gctcgccacg ttccaggctg gcgaccatgc
     1621 cttcgacgta caacacggca tcggccagct tgtgcagctc cagaggcgcg ggggcgctct
     1681 cctcgctcca gctcgcgaca actggcaatt gagcactcag cgagttgccg gccgagctca
     1741 ggccgaccat gccgagggtc ttgctcagtt tgccgagcaa ggcgtgcagg ctgttgaggc
     1801 tgtcgctttg caaggtgccg cgctcaatca ggtcgagcat gtccttgacg ctgttgagct
     1861 cttcacggat cgccgagctc agcgaacgca tgaccgcctg cccagggcct gccaggcgct
     1921 ggtattcctc ttccagcagg tgatcggtaa agggcaacgg cgtcaggccg aatacttcac
     1981 ccagggctgc ggcttgcggc ccgcggctat ccgccagggc caccagatag agcaattcct
     2041 tgagcaggcc acgcggcgct tcgtactgcc cattgacgag catctgcttg agttcgcggt
     2101 caatccgcga gaacagctgt ttacgcgact tgcgcgccag cagctggccg tcgacctgcg
     2161 cctcaacggc agccgagcca acccagcaca ggcgaccacg tggctcattg gcaaacaggc
     2221 tgtcgagacg ggtcagtgcg cggcccatca gcttgaggct ggcttgcggg ttctgctcac
     2281 gaataaagcc cagcagccct acctgataca tatggcgcag acggcgccct tcactttcgc
     2341 gtgccgcacc atcaaccgat ggtggcgagg tacgcgggcg agcatgatcg aggcgcacgc
     2401 tgaaaaagaa gctttccggc aaggcgggtt gaccgccggc ctgacgcagg tcgttgatcg
     2461 ctggcagcag caactccggc atttcctggc gatgtgcttc aacgttttcc agatagcggc
     2521 gcagtacgtg cagcgcgttg ctcagggcgg aaagctgcac atcgcgctct tcaccggcgc
     2581 cggcgggaat atcggtggcc tgatccagca cttcctgagc cagcagctcg gcaccggcca
     2641 gttcgatcag attcagtgtg ccgcgtacct gatgcaggtt ttccacggcc tgttgcagca
     2701 ggctgccgtt gtgacgatcg gcgataaagt gttcgaggct cgactctgcc tcttccatgg
     2761 tggtgaacag ttcatcgcgc accagattaa gcgacgtggc tccagaaacc atgagctatt
     2821 gcgcctcccg cgtcggtgat aaaaacaggt ggaaggacag catcagtcag cgaactccgg
     2881 tttctgcttg ctcatatgag ccgtcatggc cacccgtaga tcggccgatt gcagcatggc
     2941 ggcgttccag gtggcgatat attccaggcc atcgtcgacc cgatgatcac gcatgtagcg
     3001 cagcatttcc ttggtaccgc gaatcgccac cggcgacttg ctggcaatct cgcgggcgat
     3061 gcccatcaca ccatcgagca aggcatcagc atcggcatag gttcggttga ccagaccaat
     3121 gctacgggcc tcttcaccat cgatggtgcg accggtaaag gccagttcac gcatcatgcc
     3181 gtcaccaata atgcgcggca ggcgctgcag ggtgccaacg tcggcagcca tgccgatgtc
     3241 gatttccttg atcgagaact gcgcatcagc cgtcgagtaa cgcatatcgc aggccgagat
     3301 cagatcgatc gcgccaccca ggcaataacc ttgaatggcc gcgagaaccg gcttacggca
     3361 gttgtctaca gcattgaacg aggcttgcag ttcgagaatc ttgcgccgca gcttttcggc
     3421 attgcgacca acatccgggc ccagctggct gccgaccgag gccagcatca tcaggtcgat
     3481 acctgaggaa aagtgtttgc ctgcaccgga gatcaccact gcgcgcactt catcggtgtc
     3541 atcggcccag cggaaaatct cgatgatttc gcgccagaag tccgcgttca tcgcgttgac
     3601 tttttccggg cgattgatca ccacatgggc gaccttatct gccaacacga cattaaaggc
     3661 tttgtagtcg gacacggcag tcatcctcag ctggggtgca agcgcacaat gaaaattttc
     3721 ttatgctaaa aatcgagcaa ctataacaac caaccgacaa atgtcgatgc cggccactgt
     3781 gacgcagaac acgtcatcaa agttaagctc aattttgact tatcggcgat aagcgcttga
     3841 tctactgaga aagactggcg gcaagccgac gaataacgcc agtgccgcca ggcaaagccg
     3901 caggccaggg cgggatcagg ccttgatgca gtccacggtg tagacgcctt cctcacgctg
     3961 caggccgtgc acatcggcat cgaaaccggg gaagctgcgg tcgaaggtgc gggcaaaggc
     4021 caggtaatcg atgatcgaac gggtctgcgc ggtaaagcgc tcacccggca tgatcagcgg
     4081 aatacctggc ggatagggca ccagcatcac cgcggcaatg cggccttcca acagctcaat
     4141 cggcaccgcc tctacttcac cacgcaccaa ttggttgtag gcatcggccg gcttgatcgc
     4201 cacttctggc agcactgtgt acatgcgctt gagcgccttg gccgtggcgt tttcgcggta
     4261 gcaggcatgc aaggcatcgc acaggtcgcg cagacccatg ccctgataga aggtcttgcc
     4321 ggcctgggca atcgaaggca gcacatcact cagtggcaag ttggcgtcat agctgcgttt
     4381 gaactccagc aactcggtca gcagcgtgct ccacttgccc ttggtaatac ccatggagaa
     4441 cagcaccaag aaggaataca gaccggtctt ctccaccacc aggccacgct cccagaggaa
     4501 cttactgacc actgccgccg gaataccgcg tgcgtccagc ttgccatcgg cggtcagccc
     4561 cggcgtcacc agggtcacct tgatcgggtc gagcaacaca taatcttcgg ccacatcgcc
     4621 aaagccatgc caatcggctt gcggctgcag caaccaatcc tgggtcacca catcatcggt
     4681 gccttcggcc ttggccggct gccagatgct gaaccaccag tcatcgctac tgaggttttg
     4741 ccgcaggttg gccagggcgc gacggaagct cagggcttca tcgaaggttt cctgaatcag
     4801 cgagcgaccc gccggccctt ccatcatcgc cgaggccaca tccagcgagg cgatgatgcc
     4861 gtactgcggc gaggtggaga tgtgcatcat aaaggcttcg ttgaagcgat cccgatccag
     4921 ttggcgcgca ccaccatcct gcacatggat catcgaggcc tggctaaagg ccgccagcag
     4981 cttatgcgtc gagtgagttg taaacaccag cggcgcatcg tcggtgcgcg cggtgcccat
     5041 gccgtaacgc ccgctataga actcgtgaaa cgccgcatag gcgtaccagg cctcgtcgaa
     5101 atgcagcact tcgacgctat tacctagggt ctgcttgatc agctcggcgt tgtagcagag
     5161 gccgtcgtag gtcgaattgg ttacgaccac cagcttgacc atcgctccgc tggcgctcgg
     5221 cacccgacca cgggcaagcg ggctggcatc gatcttcgcc tgaatcgact cgcggctgaa
     5281 ttcactcagc ggaatcggcc cgataatccc cagctcgttg cgctccgggc agagatacag
     5341 cgggatcgcg ccagtcatga tgatcgaatg caggatcgac ttgtgacagt tgcgatccac
     5401 cagcaccagg tcatctcgcc caaccatgct atgccagacg attttgttgg cagtcgaggt
     5461 gccgttgatc acgaaatagg tgtgatcggc gccgaagtta cgcgccgcac gcgcctcggc
     5521 ctcggccaaa gggccagtgt gatcgagcag cgaaccgagt tccggtaccg ataccgagag
     5581 atccgagcgc agggtgtttt caccgaaaaa ctggtgaaac gcctgcccca ccgggctctt
     5641 gcgataagcc acaccgccgc catggcccgg cgtgtgccag gagtagttgg actgcgcggt
     5701 gtgctgcacc agcgccttga agaatggcgg caacaggcaa tccagataat tgcgcgcggc
     5761 ccgggccact tgacgggcga ggaacggtac ggtgtcttcg aacagataga gaatgccgcg
     5821 caactgattg aggtcgacca tggcctcggc cggtgcgttc tcgatggtca cctgctcacc
     5881 gagggcgaaa atcggcaact gcggcgcacg acggcgagcg atgcggatca gctcgaccat
     5941 gtcctgcagc aggttcttgt tttcacccgc gccttcggcg gcgaccagaa tgcaagccag
     6001 gccgtgatgg gtagaggcaa cgatgcgccc ctcggcggag ctggcggtgg aaagaatgct
     6061 gaagccgtcc tgctccagtt cttgggcgat ggcgcgtacc cggtcaccgg ccacggtatc
     6121 ggccttgatg tcacggtgca cgatcaggac ggggaacttg aggtctttat acattgtggc
     6181 gtccctggga gtgacaggcc ctagcctgcc tatcgcctca gggtagaggc tggctccgcc
     6241 aggcggaacc ccctctgcgt gacgctgtac gaaaaggtcg cgagtggata actcgcgacg
     6301 tggttaaagc tcggctgcgg gctgctccat ctgcacccaa agcggtggcg cacccgcgga
     6361 tttttcgata atcgccagac gcgctgcatg ttcggccagc tcagcagcac tggcctggat
     6421 cacccgtgta cgcgcccgct cagcgggcaa gcggcggatc gcactgggtt gctgacgacc
     6481 attgctgtcg ccttccgaac catcaccggc cagggacaga ttggtctgcc caccggtcat
     6541 ggtcaggtag acgtcggcga gaatctcggc gtcgagcaag gcaccgtgca ggtcacggcc
     6601 ggagttgtcg acgccatagc gcttgcacag tgcatcaagg ttgttgcgct gccctgggtg
     6661 gcgctcacgc gccatcatca gggtgtcgag gatcgagcag tgctcgctga tatcagcgcg
     6721 gtcgtcctgc ttgatcaggg cgaattcgtt gttgataaag ccaacgtcga acgccgcgtt
     6781 gtggatgatc agctgcgcgc ccttgataaa ttcgaagaac tcgtcggcca cttccttaaa
     6841 gcgcggcttg tccttcaaag catcattggt aataccgtgg acggcgattg cgccttcatc
     6901 gatctcacga tcaggctgca ggtagacatg gaaatgcctc ccggtcaggc gccggccgat
     6961 cagttcgaca caaccgattt cgatgatccg atgaccatcg gccaccggca tacccgtggt
     7021 ttctgtatcc agcaccacac tacgcatgct tcaaccctct tacttcatca acaccgcgat
     7081 tggccaactg gtcggcgcgt tcgttacccg gatgaccggt atggccgcgc acccactgcc
     7141 acgtcacctc atgacgatta acctgctcgt ccagcgcctg ccatagatcg gcattcttca
     7201 ccggctcttt ggcggcggtt ttccagccgc gcttcttcca gttcggcatc cactcttgga
     7261 tgcccttcat cacatactgc gagtcggtaa tcaggcgcac cgagcagggc cgagtcagcg
     7321 cgatcaaggc acaaatcgcc gccatcagct ccatgcgatt attcgtggtg ttgggctcac
     7381 cgccccacag ttccttctcc acacccttgc aaaccaacaa cgcgccccag ccaccgacgc
     7441 cagggttacc cttgcaggca ccatcggtgt agatctcgac ctgttcactc atgtatctgc
     7501 aatctctcgc tcaattaatc ggccgatgtt gttcatctgg cgtttcgcgc cggctgactt
     7561 tggccacggg cataggaatc aatttgccaa cagcctgccg gctgggttgg cgcaatggcc
     7621 gcaaccccac caccagcttg cgcgccacca gcacataaaa gccgccaccg ggtggctgcc
     7681 aggccgcgcc cagtcgctcc aacccagcca gtcgcgtttg ccaggccggc gcagaaagcg
     7741 gcggacgata gcacccgaag cggcgtttct ccagcgcgaa gcccagcagg tgcaaccagt
     7801 cgctgacccg attgggcgca atacaacggg cctggcgcag cgcatcacga gtaaacagat
     7861 gtcgcagccc ccaactgctc atcggattga tcccaacaat cagcagatgc ccacccggcc
     7921 gcacgctgcg cgccgcctcg cgcaacaggc catggggcga caagctgaaa tccagaccat
     7981 gctgcaacac caccacatcg gcggcatgtt cggtcagtgg ccaggagtgc tcctcacaaa
     8041 cgatctccac gcccttgaat ggcgccccca gccgcacgct gcgctgaatc tgctgggcat
     8101 ccggcggcgt gtcggcggct gggccgtaat gcactaggta gccgccgaaa aagcgcgcca
     8161 gctcttcctc taatagttgc cgctcctgct ccagcagcaa ctgcccgagc gggccgctca
     8221 gccaactgcg cgccgcagcg ataagatcga gccattcact gtctgcgagg gcaaatggat
     8281 gatcggacat aaggactgct cccgtttctt cgccacggca ccaatgtagt cagcgccgcc
     8341 gatgagacct aagatgcacc tttgtaccct gcttagcgac ccgctcatga ttaaaatcga
     8401 cgccctgcct gcattcactg acaactatat atggctgctg caagacgcca aacagcgccg
     8461 ctgcgcagtc gtcgaccctg gtgacgccgc gccggtcgaa gcctggctgg cggccaaccc
     8521 cggctggcag ttgagcgata ttctgatcac ccatcatcat ttcgatcacg tcggcggtgt
     8581 tgaaaaactg aagacggcaa ccggtgctcg ggtactgggc ccggccaacg aaaagattcc
     8641 agggcaggat ctggcgctgc gcgacggtga ccaggccgac gtgctgggcc agcgtttcga
     8701 tatatatgaa gtaccagggc acaccctggg gcatattgcc tatatccagc ccaggcagca
     8761 ctggctgttc tgtggcgaca cgctgtttgc tggcggctgt ggtcgtctgt tcgagggcag
     8821 ccccgagcag atgtacaact cgctggaccg cctggccgcc ctccccgaac agactcaggt
     8881 gttctgcacc catgaataca ccctgagcaa cctgcgcttt gccgcggcag ttgagccgga
     8941 caacgcggaa atccaacaac gcctggcgca ggtcagccaa tggcgcgagg ccggacaaat
     9001 cagcctgccg tcatctattg gtctggagcg cgccaccaat ccttttctgc gcagcgcaca
     9061 agcgtcggta ggcgcccgca ttaatgagcg cgaagggccc gccagccgat cgccagcgca
     9121 ggtgttcgcc gctttacgcg cctggaagga ccatttctaa acctcgacaa ccacacaaaa
     9181 gactggttaa aacttgacca agggcaggct ggttcctaga atcgcccgac cttttcgccg
     9241 ggaagtacac caccgccaat gcctctatca ccacgcaaga ccttgaattt aaaggcattg
     9301 gcacaaagcg ctcagacgct tgtggtgatc atgtgcgtaa ccctggccgg ctgccagacc
     9361 agcaaccaat accgcccgga cagcggccgc gacaccgacc gtgcggtcgg ccttgagcaa
     9421 gagccagaat ggctcgacag cagcgtggtg gcgccccccg aagcgccgaa agacatctgg
     9481 gaacgggtgc gcaacggcta ccagctgcag gacaccatca ccctcaaccc gcgtatcgag
     9541 cagcagcgtc tgtggttcgt cagtaacccc tcgtttatcg agaaagccgg cgagcgcagc
     9601 agcccctata tccactttat cgttgagcgc ctggaacagc gcaatatgcc gatggagctg
     9661 gcgttgctac cgatgatcga aagctcctac aacccgctgg cctactccca tgcccatgcc
     9721 gtcgggctgt ggcaattcat cccatccacg ggacgtaatt tcaacctgcg ccagaccaac
     9781 tggtatgacg gacgccgcga tgtgatggcc tcgaccgatg cggcgatcag ctacctgacg
     9841 cgtctcaagg aaatgttcaa cggcgactgg ctgctggccc tggcggctta taacgctggc
     9901 gaaggccggg tcagccgggc gattgagcgc aaccagaaac tcggcctgcc gaccgattac
     9961 tggaacctca cgctgccggt ggaaacccag aattacgtgc ccaagctgct ggccctctcg
    10021 caagtggtga tgacgccaca agcctatggc gttagcctcg cgccgatagc caacgagcca
    10081 tatttcgaga aggttgtatt cacccaacgc atggatctcg cgcgcgtcgc cgccatggcc
    10141 gacctggatg aagacgagct gtacctgctc aaccccgcgt tcaagaaagg cgtgaccctg
    10201 gatggcccgc aacacttgct ggtgccgacc gacaaggccg agctgttgac cgccaacctg
    10261 tcgctgatga agccgcaggc actggtggac tggcagcaat accgggtgcg ttcaggcgac
    10321 agcctgcaca gcatcgccaa tcgccatcag ctgaccgtga acaccctcaa ggacatcaac
    10381 aaactgtccg gcaacaacct gcgcatcggc cagatgctca gcattcctgc gcaacccggc
    10441 gtgccggcac gcgagccaat gttccagcgc agcgtcgcac atagcgcgcc gagccgtacc
    10501 taccgagtaa gaagcggcga caacctctgg cagatcgcca agaaccacaa ggttgctgtc
    10561 aaggatgtgc agcgctggaa caagctttcc ggcaatagcc tgcgggtcgg tcaggtgctc
    10621 accttgcaag ccggcaaacc tagcagtggc gggagcggta gcagtggtaa ctcgtcgcgc
    10681 cagagcgcga cctattacaa ggtgcgtcag ggcgactcgc tgtacctgat cgccaaacgt
    10741 ttcaaggtac cgatgaagag cctacagaac tggaacccca aagccggcaa ggcgctcaag
    10801 cccggccaga ccctgacact gtacagcgtc aactgatcag gccttcttgc tcatcgcctc
    10861 caagcagcgc ccggtcaact gggtaaagcg ctgaaaagcg acaaactgct catgcttcaa
    10921 cgctcgccca gatacccggc tgtcggcata taacaaacca atttcgcggg tgccagccaa
    10981 caggggcgca ataaagaaca taccctgccc caggcgctgg cgaatcggct gggtgaccag
    11041 ctcattaagg ttgtaactgg ccggcacgcc catccacaac gcttctttat tacgcagcgc
    11101 gtaactgaaa atatgcggct gctcttgctg cgccacgggc aggacgaagt cacggctcca
    11161 gccttcggtg ccttcgccaa tcacccgctt ggcgcggaaa caactctgcc cgtccgccag
    11221 caccaccagc atgatgcgct ccagtcccgt cccctgatgc aggcccttga agagggtgtc
    11281 gagcaccagt ccaagatcgc cgcggcgcga aaccagcagg ccaaggtcct gcaaagcctg
    11341 ctgcaaaatc agcagattgg gttgcagcaa ccgcgcctta cgctgttcct gctgcaggcg
    11401 aatctgttcc gggtcggtat tggggattag cttgcacagc tggctagcgc caaaggtcgt
    11461 tgccatcttc acagtttcct cagcgctggc cagcacctgc tgcatggctt cctgcggggt
    11521 caccccaata aaactggcca actggccgac caacgcctcc atgactggcg tatcccagcc
    11581 atccaacgct gcctgactga tctttgcccc cagggtcaca gctttgaccg ccggatcatt
    11641 ctgactggtt gactgatgcg ccaggctgac ggtatcgccc aggttccagc ttttcaccag
    11701 ggcctggctc agttggcgga aactggtgcc cagcatccgc atcaccactt cgtcttcatc
    11761 cgccccaggc tgggccagag cgctggccag gtcatcggcg tcatcaccgg cacaacccca
    11821 gaacgccagc tcgccgacgt tgtgcagcaa ggcggcgata aataccactt catcgttttt
    11881 agaaagcaca tagccggcga tattgcgggc ctgcacagcg gcgtgaaacg agcgtgccag
    11941 caattcagca agctggtcgc gggagctacg cgccagcagg ccatcgatca ggctgaccga
    12001 tagaccgatc tggcgcacgt tatcaaagcc aatcagcacg atggcccgag aaatggtgcg
    12061 tataggttcc tgggaagggt tgtaatagac gctattgccg atgcgcagca ccttggtggt
    12121 cagcgccgcg tcacgcagca atacatcggc caattgttgc accgaggatt tgtcgccttg
    12181 ggtaagccgg tgtaaatcct gcaccaccgc agacagggcg ggtaattcgg cttgggtgaa
    12241 acggtcaatc catgactgca gaccatggca agctgtagcc aagttaatac cctcgcgatt
    12301 agtaattgca gtgtagtcaa agtgccgcta ttgcttgccg gcgatcctac cctttttcca
    12361 gccgatacaa gctgttaatg tgcctgacca gcagcccaca agacgccacg gatcggatat
    12421 ttcacttgat acgtcccctc gtctcgctac ttctctgcat ggccatcagt tcatctgcct
    12481 gggccactct gtataaaagc cacggttacg cccagtttgg cgagctgaag tatcccgcca
    12541 gctttaccca tttcgcctgg gtcaacccgg aagcccccaa aggcggcacg ctgcggatga
    12601 tggccaacgg cacattcgac acgctcaacc cctataccct caagggcagc agcccgatca
    12661 gcagcgccaa ctttctgcaa tatggcgtca acgagctcaa cgcgccgttg atggtcggta
    12721 ccggtgccta tgacccgtcc ggtgacgaac ctgcctccag ctacggcctg atcgccgagt
    12781 cggttgagta cagcgatgat cgcagctggg tggtgttcaa cctgcgcccc gaagcgcgct
    12841 ttcatgacgg caagccgatt accgcctacg acgtggcctt ctcctaccgc ctgctgctca
    12901 aggagggtca tccgcagtac cgcaccaacc tgcaggaagt aaaacgggtc gatatcctca
    12961 atcgccacag catccgtttt gttctcaagc gtgccggcaa ttccctgttg atcctgcgcc
    13021 tcggcgaatt accggtactg ccgcagcact actggaaaga ccgcgacttc aaggccacca
    13081 cctatgaacc gccgctgggc agcgggccct accggattac ccaggtcagc cccgggcgca
    13141 gcctgaggtt tgagcgggtc aaggattggt ggggcgccaa actgccggta aaccgcggca
    13201 aatacaattt cgaccgggtc gacgtggaat tctaccgcga cagccatgtg gccttcgagg
    13261 cgttcaaggc cggtgagttc gacatctaca tcgagcagca ggcgaaaaac tgggccaacg
    13321 gctaccgctt cccggcattg agccgtggcg aagtgatccg cgccgagatc ccgcaccaga
    13381 tcccaaccca gacccaagcg ctgtttatga atacccgccg cgaggtgttt gccgaccgcc
    13441 aggtacgcga agccctgggc ctgatgttcg acttcgaatg ggccaaccgc gcgctgttca
    13501 acagttccta cacccgtgcc aaaagctact accccaacag cgaattttcc gcccgcgaca
    13561 aaccggaagg cgccgaatgg ctgttgctct cgccctaccg caagcagtta caggcgcgcc
    13621 tgttcagcga gccattcagc atgccgcaga ccgatggccg gggtatcccg cgggaaacct
    13681 tgcgccgtgc cctcggtcta ttggccgaag ccggctggaa gccatccggc caacgcctgc
    13741 tcaatagtga cggacagccg ctgcgattcg agatattgct agtcaatccg aaccttgagc
    13801 gcattctcca gccctttacc gaaaaccttg ccagcattgg catccaggcc aacctgcgta
    13861 ccgttgaccg cgcccagtac aagcagcgcc tcgatcaata cgacttcgac atgattctgc
    13921 tcaccctgcc gcaaaccctc agcccgggcc tggaacagtc gctgtatttc cattcgtccc
    13981 aggccaagat caaaggcggc aggaattacg ccggcgttac ccacccggtg gtcgacgcgc
    14041 tgatcgaaaa actgctgtcg gcgcagagcc gtgacgagca gatcgccgcc acccgcgccc
    14101 ttgaccgggt actgttgtgg cagcactaca gcattccgaa ctggtatatc gattaccacc
    14161 gcctggctta ccgcaaccga tttgccttcg tcaccacgcc gccctacacc ctgggcctgc
    14221 gtacctggtg gctgaaatcc atggagaacg ctgaatgacc catgcattgc gcgccc
//
LOCUS       DASRUM010000058        19576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1037789, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000058 DASRUM010000000
VERSION     DASRUM010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 19576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19576
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037789"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VFF22_04255"
     CDS             <1..569
                     /locus_tag="VFF22_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HZX16069.1"
                     /translation="LIATFLLPVLAGLPLPFSPIQIILLELFMDLAASAGFVAEPAEA
                     DIARRQPRRAASALLDGAAVRTILFKGALLFAAVMAAYAWANWRGLPPAAVQSCAFAA
                     WMVGHVALAFISRSDRDWIVRHGLFANRVMNLWAVAAIGFLLLAIYLPPLREALRFAP
                     VAPVDLIVSAVLALLWVAPAELRKAFVR"
     gene            674..1218
                     /locus_tag="VFF22_04260"
                     /pseudo
     CDS             674..1218
                     /locus_tag="VFF22_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246759.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
     gene            1343..1768
                     /locus_tag="VFF22_04265"
     CDS             1343..1768
                     /locus_tag="VFF22_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZX16070.1"
                     /translation="MHFIKNIRWTLFFLIVAVSIGAYSILVAYPPSKGKQGRAKAVAS
                     EPISAAEELNAVALYSMTCAACHGQRLEGGVGPSLVGIGSRIPPTKIERIAQRGKGKN
                     KPVSMPPGLATPEEARLLARWLAAGPKLAVDAPETGQAK"
     gene            1870..2337
                     /locus_tag="VFF22_04270"
     CDS             1870..2337
                     /locus_tag="VFF22_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16071.1"
                     /translation="MSHKHCKAALLGLVASVALASAHLTLAAGPQIETFTLVSGAEVG
                     PNGKSYFTAGNGHHGFFGRGGTIDGQPNPTLRVKQGDTVKITLLDLDQRGQPAQLAVP
                     DLGLATPTLTRSGQSDTLNFTASRAGTFRYSGAIAFDGHNHQHMVGEIVVETK"
     gene            2378..3649
                     /locus_tag="VFF22_04275"
     CDS             2378..3649
                     /locus_tag="VFF22_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZX16072.1"
                     /translation="MSRKTLKWLGLAALAAIIVAGAGAAYWRQQAAQADAYTTVKVTR
                     GDIRDTVSALGTLVPSQYVDVGAQVSGQLIELPVKLGDTVKQGQLVGVIDPTPYTAKV
                     AQDRAQIADLQAQLAQSQATLALARWTYASNSTLAAQGAATGQAREQSQANLKVAVNA
                     VASLQAQIDKANNILKVDQANQGYTRIVAPIAGLVISPTSANYGAAWNKLDIARRGQT
                     LNANQNAPVLMRIANMDRMVVRAQVSEADVARLKTGMPVDFITLGRPDRRIKAQLGAI
                     EATPELVNGAIFYDAVFEVPNADHSLLPQMTTQVFFVVAQASNALIVPLTALSSIQRQ
                     NGVGMPGCPAAAAVGNADCVQVLIGGKPVARTVKVGVKDEVNAQILEGLEAGDQVIVG
                     ATGTTAASGSGSGTGGGKGKGKGAGSDGGGQ"
     gene            3633..5603
                     /locus_tag="VFF22_04280"
     CDS             3633..5603
                     /locus_tag="VFF22_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HZX16073.1"
                     /translation="MAAGSEGARVTPLIELRDVVKRYAQGDLSIEVLHGISLTIQEGE
                     FVAIMGASGSGKSTLMNIIGCLDRPTSGHYRVLSRDVAELDSDALAELRRNTFGFIFQ
                     RYNLLGSASAQENVEVPALYAGLAAGPRRERARLLLTRLGLGERLDNLPGRLSGGQQQ
                     RVSIARALMNGGRIILADEPTGALDSQSGKEVMELLRELHRAGHTVVLITHDPKIAEE
                     ADRTVCVSDGHIVEDTGERVARRTEAVLPAPSVGPTGSVRSVLMLDIMASVRTALRAL
                     RASRVRTFLTLLGVMIGVGSVVAMLAFGNGARQQVLDQIQALGTDLLLVKPGAPGVRG
                     AGGTTTLVPADAEALVRLPNVGYVVPQTSNPVTVRYGNATYTTTLYATTPAFTAANNW
                     PVARGTFISQANLQGFAPVAVLGQSTAKALFSAADPVGRYVLVNNTPLRVIGVLAAKG
                     ADQQGNDRDDAVFVPLTTGQARITGQSYLKFITIQIDDTQQIAATEAAINALLIKRHR
                     TQDFQVRSMTALLEQQAQTQQSLTLMLGVVALISLLVGGIGVMNIMLVSVTERTREIG
                     VRMAVGARTFHILLQFVTEALVVCTIGGLIGVAGGLLVAGVAAWSGAAVLVTLPPVLL
                     AFTSALATGLVFGYLPAKRAAALDPVVALSTE"
     gene            5606..7003
                     /locus_tag="VFF22_04285"
     CDS             5606..7003
                     /locus_tag="VFF22_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZX16074.1"
                     /translation="MIDLLNMQRWSGLIAALLLAGCAVGPTYERPAVESPSDWVADKA
                     DARWPAIDWWTAFRDPVLDRLVIEALANNHDLKAAVQRIAQARALAQVAGAAHYPSLS
                     ASAQASRQNNSNNNLNGKFVTPNSYQAGLTASYVLDLFGLNRDQAEAAAAKLKGSEFE
                     RQTVELGVAAVTASSYFKFAALDARLAVAKETLAKARHAAELVRTRQQAGMATALQVA
                     QQQAEVASLQAAIPTLQTQRRQVLNALAMLAGHLPDGFDVKPTAIAQLGLPQAPAGLP
                     SALLERRPDILRAEAALAGANADIRAATAALFPRINLTAQGGYASAALRQLFDPGNTF
                     YSLAAGLTAPLFEGGKLRGELAYTEARYGELVQNYRQTVLAAFTDVENALTAQRNTDD
                     TLVAQRDAVTQATLAYSLAEFQYEQGMVDYLAVLTAQRSLLAADDAEAQARFARLDAA
                     VSVYQALGGGWNIPQ"
     gene            7127..8029
                     /locus_tag="VFF22_04290"
     CDS             7127..8029
                     /locus_tag="VFF22_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX16075.1"
                     /translation="MELRHLRYFIAVAEELHFARAAERLHIEQSPLSRAIKDLEYDLG
                     VQLFERTTRSTRLTWAGQVFLDEARRVLSTLEQAKASAKAAATGYRGTLRIALSDGIA
                     PPRLAALLAQNREEEPEVEIRLFETTLAQQLKGLCSGLYDAGFAHAAEVGEDIIAEPV
                     WTDPLVIAVPARHPLLAHKRIPLEELIRYPLVLCHPEACEGCHRQLERILRTANVEPI
                     VAEHVTTFNLMMILVAAGYGLGFANESQMTVCRHPEVVARPLAGRALMLSTYLLRPNT
                     ESSEPLSRFIARALETTVSPAITK"
     gene            8041..8283
                     /locus_tag="VFF22_04295"
     CDS             8041..8283
                     /locus_tag="VFF22_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014859551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HZX16076.1"
                     /translation="MKTFPALLLAIVLTACGQSAPTDTVDSLAANPERLKELRQQCKA
                     DRAKLGDALCNAVAEATRKRFMGNGTPYTPESAPKN"
     gene            8566..10557
                     /locus_tag="VFF22_04300"
     CDS             8566..10557
                     /locus_tag="VFF22_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HZX16077.1"
                     /translation="MQGTSVLFGQVLTVFCIVIAGVWGATQWTAAALGYQLRLGTPWF
                     DFFGTSIYHPWRLFEWWFFFDAYAPHIFNTGGAIAGASGLVAMVVAIGMSIWRSRQSK
                     LVTTYGSARWADAKEIRKAGLTGPAGVFLGLHDSQYLRHEGPEHVLTFAPTRSGKGVG
                     LVVPTLLSWPASAVIHDIKGENWNITAGWRSRFSHCLLFNPTDPRSAAYNPLLEVRRG
                     AHEVRDVQNIADILVDPEGALERRNHWEKTSHALLVGAILHVLYAGKDKTLRGVANFL
                     SDPACPFEVTLHRMMSTRHLGQSTHPVVASAAREVLNKSDNERSGVLSTAMSFLGLYR
                     DPTVAEVTSRCDWRIADLISAEHPVSLYLVVPPSDISRTKPLIRLILNQIGRRLTESL
                     DGSDGVERKHKLLLMLDEFPALGRLDFFESALAFMAGYGLRAFLISQSLNQIDKAYGQ
                     NHSILDNCHVRVTFATNDERTAKRISETLGTATELRAQRNYAGHRLAPWLGHLMVSRQ
                     ETARPLLTPGEVMQLPPDESVVMVSGHAPIKAKKLRYYLDANFKRRVLPPPTLTFGRY
                     ADVPAARADDWSALAVPTVPATSTTVGLGEQADAGGPRQQPELSEIATYSPEPEQPAS
                     DLSLLDDDDFSLPLPSQLDPRLQRTARLAALDPDDGIQL"
     gene            10554..11006
                     /locus_tag="VFF22_04305"
     CDS             10554..11006
                     /locus_tag="VFF22_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012076308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HZX16078.1"
                     /translation="MSQARLNLFIQHDHAKRLDELAAKKGVSKSSIVAAALASWLSPD
                     AGDQREAATAKRLDRLSRQFERLERDQNILIETLALYVRYYLTVSTPVPEAHQEAAKA
                     QGKARFEQFIEQLGRHVLRGRSLVKEVYEEIQPDATRLAEAAMQEDGA"
     gene            11003..12052
                     /gene="trbB"
                     /locus_tag="VFF22_04310"
     CDS             11003..12052
                     /gene="trbB"
                     /locus_tag="VFF22_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HZX16079.1"
                     /translation="MSAVPKLPPELQLKPPSSAATSLDRRIRMLRTAMGPLIAAALED
                     PDVVEIMLNPDGSLWIDRLSSGRSPTGVKLSEADGERIIRLVAAHVGTEVHRGQPLLT
                     AELPETGERFEGILPPAAPGPAFALRKRAVSIIGLERYVADGILTEEQAEFLRRAVRE
                     RLNILIAGGTSTGKTTLANALLAEIAATGDRVLVLEDTVELQCAARDHVSLRTRSGVV
                     SMAELVRATMRLRPDRVIVGEVRGGEALDLIKVWGTGHPGGIATIHASSAHGALLRLE
                     QLILEVALTPPRALIAEAVNVIVFIAGRGRARHVQTIARVVGFDGHGYQLDDALAPPL
                     PTISLPSSTTPGELP"
     gene            12049..12408
                     /locus_tag="VFF22_04315"
     CDS             12049..12408
                     /locus_tag="VFF22_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019485843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HZX16080.1"
                     /translation="MTHTVFSTSRFSVNPHLRLTLHVAVLAVLMLCLATTAHAAGSSM
                     PWEGPLQSILESIQGPVARIVAVIIIISTGLALAFGDTSGGFRKLIQIVFGLSIAFAA
                     SSFFLSFFSFSGGAVVA"
     gene            12405..12689
                     /locus_tag="VFF22_04320"
     CDS             12405..12689
                     /locus_tag="VFF22_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HZX16081.1"
                     /translation="MSAANEFAAGFASGFEIPLHRSLTEPILLGGAPRTVAIANGTLA
                     AAVGLGLQLWIPGLVLWIVGHSLAVWGARLDPQFMVVFSRHIKHRPLLDV"
     gene            12711..15161
                     /gene="trbE"
                     /locus_tag="VFF22_04325"
     CDS             12711..15161
                     /gene="trbE"
                     /locus_tag="VFF22_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HZX16082.1"
                     /translation="MMNLAEYRKRPALLADWLPWAGLVAPGVVLNKDGSFQRTARFRG
                     PDLDSATQGELIATSARLNNALRRLGSGWALFVEAERRAAADYPHSEFPEPLSWLVDE
                     ERRASFEEDHSHFESSYHLTLVYLPAEESRARAAKLLYENTPQGGVDWRERLESFISE
                     TDRFFDLLDGVMPEIVWLDDSQTLTYLHATVSTRHYPVAVPEHPFYLDALLADSALLG
                     GLAPMLGDQHLRVASVRGFPTSTWPGLLDDLNRLGFAYRWSTRFLCMDKSEAEKELTR
                     LRRQWFAKRKNVVALLRETIFQQESPLVDTDASNKSADADAALQELGSDQVSFGYVTV
                     TVTVLDADAGAADEKLRRIERVIQGRGFVTIPETLNAVEAWLSSIPGNAYANVRQPII
                     STLNLAHLMPVSAVWAGPERNDHLDGPPLIVTRTDGATPFRLVTHIGDVGHTLVVGPT
                     GMGKSVLLAALAMQFRRYPGSRIFAFDMGRSMRATILGLGGEHYDLGTDGEIAFQPLA
                     RIDQEGYRTWAAEWLEGRLLHEGAAVGPEEKAAVWSALGSLAGAPVEQRTLTGLSVLL
                     QSNALRQALQPYVLGGAHGKLLDADSDRLGSGSVQCFEMEELMHSKAAVLAVLGYLFA
                     RFDERFDGAPTLLILDEAWLFLDDPVFAARIRQWLKTLRKKNVSVIFATQSLADIQNS
                     SIAPAIVESCASRIFLPNPQATEPQIRTIYEGFGLSRRQIDIVAEAVPKRDYYYQSRL
                     GNRLFDLDLGPVTLAFAGASTPQDQRAIDEIRDAVSRSVPSPDFAAAWLRHRGLDWAA
                     DLVPSFPSHSSIPQEYPS"
     gene            15158..15886
                     /gene="trbJ"
                     /locus_tag="VFF22_04330"
     CDS             15158..15886
                     /gene="trbJ"
                     /locus_tag="VFF22_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003103988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HZX16083.1"
                     /translation="MKKTILAIAAAALVTLMPTAHAQWVVIDPSNLAQNIMTAARTLE
                     QINNQIRQLQNQAQSLMNQAKNLTGLDFSALNELRASLSATNQLIQQAQGLAFNVSQM
                     ETEFQRLYPDAYSASTSGTQMATDARSRWRNSLEALRTATKVQSQAVQNFASDEQTLT
                     DLVNRSQSAVGALQATQATNQLLALQARQSIQAQQLQITQDRATALEQARIVAVQERA
                     REVRRRFIGDGTPYTPQTVDFYGN"
     gene            15896..17284
                     /gene="trbL"
                     /locus_tag="VFF22_04335"
     CDS             15896..17284
                     /gene="trbL"
                     /locus_tag="VFF22_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HZX16084.1"
                     /translation="MNDVSVIDHFLSVFSHYIDSGFGLLRGEVAFLTTTLVVIDMTLA
                     GLFWAMSHASGGGDDIIGKLIKKVLYVGTFAYIIGNFNLLAGIVFRSFAGLGLVASGS
                     TITQAEFLQPGRLAKVGIDAGAPILKQIGDMAGFPEVFVNLDAIVVLFLAWLVLIASF
                     FVLAVQLFVTLIEFKLTTLAGFVLVPFALWNKTSFLAEKVLGNVVSSGIKVLVLAVIV
                     GIGTGLFAEFQVTPNEPSIDHALTIMLAALSMLGLGIFGPGIATGLVSGAPQLGAGAV
                     AGTALGVAGAAVAVGAAASGVGGAVAAGARMAPGAAKMAASGASSMASTASSAKSAFQ
                     AGSAGAGGGLKGAAAGLGNVAKTGAQSVGQKVAAGAKSMKDRAAAAIKPDAPASGAGA
                     GAGSASTASGPATQGGNDAAPADTQQPAWAKSLHRRQQISHAASTVAHTLRGGDGGGS
                     GSSPSLRDASDS"
     gene            17296..18000
                     /gene="trbF"
                     /locus_tag="VFF22_04340"
     CDS             17296..18000
                     /gene="trbF"
                     /locus_tag="VFF22_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbF"
                     /protein_id="HZX16085.1"
                     /translation="MRFKRSLVRYADTPQPATPYQSAEQVWDDRIGSSRVQAKNWRLM
                     AFGCLVLALLMAGGLVWRSAQSIVTPFVVEVDSGGQVRAVGEAATPYKPNDAQTAHHL
                     GRFVTLVRSLSIDPIVVRQNWLDAYDYTTDRGAAVLNDYARTNDPFARIGKESVTVQI
                     TSIVRASDASFNVRWTERRYVNGAAAGLERWTAVVSVVLQTPRTEERLRRNPLGIYVN
                     GLSWSRELDSSEGAKP"
     gene            17997..19022
                     /gene="trbG"
                     /locus_tag="VFF22_04345"
     CDS             17997..19022
                     /gene="trbG"
                     /locus_tag="VFF22_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HZX16086.1"
                     /translation="MKPTLRIYVFLSLLVATSMTLSGCATQGKPPPTITLDEAVAAQP
                     LPELPKPIEVVAVPQPLPLPEQLKLLPDELEAKTAPESADEKVRVTRANQEARIAPTR
                     EGYLNAIQVWPFTDGALYQVYASPGRVTVVSLQPGEELVTVAAGDTVRWIVGDTSSGA
                     GAGLRVSVLVKPTRSGLKTNLVVTTNRRTYLIELTSTEKAWMASVSWDYPKDRMLALQ
                     RQAQAAQVAAPVDAGLSLEKIRFRYAITGSNPPWKPLRAFDDGEKVYIQFPSGIAQGE
                     LPPLFVIGAQGGGQLVNYRFRSPYYIVDRLFGAAELRLGGGSGDKGGDVVRIERTDGI
                     VSGTRRN"
     gene            19025..>19576
                     /locus_tag="VFF22_04350"
     CDS             19025..>19576
                     /locus_tag="VFF22_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006412605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbI"
                     /protein_id="HZX16087.1"
                     /translation="MSQANNAPDTPVPDVPPKVAPENVTLRSQPRPVTRLNRRMLAVL
                     AGGLATAVLGGTIWSLQSTQRRGAADQKELYNVDRVSRSEGLDRLPADYSKLPPTLPT
                     AVPQLGAPLPGDLGGPIHKAEQQAQGYGYQQPGHDPAEVERLARLKQAEEAAASSMFF
                     RSSGQKVAAPTQTAPAAQVAANAA"
BASE COUNT         3445 a         6326 c         6203 g         3602 t
ORIGIN      
        1 gcctgatcgc gaccttcctg ctacctgtgc tcgccgggtt accgctgcca ttctcgccga
       61 tccagatcat cctgctggaa ctgttcatgg acctcgccgc ctcggcgggc ttcgttgccg
      121 aaccggccga agccgacatc gcccgtcgcc agccacgccg cgccgcatcc gcgctgctcg
      181 atggcgccgc cgtgcgcacc atcctgttca agggcgcgct cttgttcgcc gcggtcatgg
      241 ccgcctacgc ttgggcaaat tggcgcggat tgccgcccgc ggccgtgcaa agctgcgcct
      301 tcgccgcctg gatggtgggc cacgtggcac tcgctttcat ctcgcgcagc gaccgtgact
      361 ggatcgtccg ccacggtctg ttcgccaacc gggtgatgaa cctgtgggcg gtggcggcga
      421 tcggcttcct actgctcgcc atctacctgc caccgctgcg cgaggcgcta cgcttcgcgc
      481 cggtggcgcc tgtcgacctc atcgtgtccg cggtactggc cctgctgtgg gtcgccccgg
      541 ctgaactgag aaaggcattc gtgagatgaa ccggcaagaa gggttgggcc gccccggcgt
      601 gcgctgtgtg ctgtcaggca cctgcaaggt attgcggcgg ggcggttcaa ttcgacaccc
      661 gagggataag tacgtgcatc gtcgtcatag tgaccgcagt tcatggtcgg tctttctcat
      721 cttcctgcgc cttggcttga cctccttcgg cggccccatc gcgcacttgg gctattttcg
      781 cgacgagttc gtcacacgac ggcgctggtt gtctgaacgc agttatgcag acttggtggc
      841 gctgtgccaa ttcctgccgg ggcctgcaag cagccaggtc ggaattgcct tgggtctgtc
      901 ccggtcgggc tacgctggag cgctggcggc ttgggcaggc ttcacgctgc cttcggccat
      961 tgccctgatc ctgcttgcgc tgggcatttc ccgctacggc gatgccatct cgccgggagc
     1021 actgcacggt ctgaaagtgg tggcggtcgc tgtggtcgcc caagcggtat ggggcatggc
     1081 gcgcaacctc tgcccggacg tgctgcgtgt caccatcatg gcgattgcgg cctgcgtcgc
     1141 cctgcttgtg ccgtctggtg ggggcaggtc ggcatgattg ctgccgctgg catcgccggt
     1201 ctgctgctgt tcaagcctgg tgcggcggtg ttattgagtg atttactgcg tgtcggcagc
     1261 ggccgatggt cggttaatta ctggctttcg caaaaattcg aggcagccgg caagtccggt
     1321 tcgaagcgtt gaggagatga acatgcattt tattaagaac attcgctgga ccttgttttt
     1381 cctgatcgtg gcagtgtcca taggggctta ctccatcctg gtcgcttacc cgcccagcaa
     1441 gggtaaacaa ggacgcgcca aggcagtcgc atcggaaccg atatcggcgg cggaagaact
     1501 gaacgccgtt gcgctgtatt ccatgacctg cgcggcatgc cacggccagc ggctagaggg
     1561 cggggtcggg ccgtcgctcg tcggcatcgg atcacgcatt ccccctacca aaatcgaacg
     1621 aatcgctcag cgcggcaaag ggaaaaacaa acctgtttcc atgccccctg ggcttgccac
     1681 gcctgaagag gcgcgcctgc ttgcgcgctg gctggcagcc gggccaaagc tcgccgtgga
     1741 tgcgcccgag acaggtcagg cgaaatgatg ctccgacttg ccggtcatga tttatgggtg
     1801 tcgtggccct cccttctagt gacgctgcgg gctctatgtt tcttcaatca tcgctacagg
     1861 agaacagtga tgtcgcataa acactgcaaa gcagctcttt tgggactcgt tgcgagcgtt
     1921 gcgctggcat cggcgcacct gacactggct gccgggccgc agatcgagac gttcaccctc
     1981 gtgagcggtg cggaagtcgg gccgaatggc aaatcatact tcaccgccgg aaacggccac
     2041 catggcttct tcgggcgagg cgggacgatc gacggtcaac ccaatcccac cctgagggtc
     2101 aagcagggcg acacagtgaa gatcacgctg ctcgatctgg accagcgtgg gcaacctgca
     2161 caactcgccg ttcccgatct cggccttgcc acgcccacgc ttactcgctc cggtcagagt
     2221 gacacgctca attttacagc cagcagggct ggcaccttca ggtactccgg cgcgatcgcc
     2281 ttcgacggac acaatcatca gcacatggtc ggtgaaattg tggtcgaaac gaaatgagcc
     2341 ggacattctt tgacttggtt caactgacgg aggggaaatg tcgcgcaaga cactgaagtg
     2401 gctaggcctg gcggcccttg cggccatcat cgttgccggc gctggtgcgg cctattggcg
     2461 ccagcaagcc gcacaggcgg atgcttacac cacggtcaag gtaacgcgcg gcgatatccg
     2521 cgacaccgtg tcggccttgg gcacgctggt gccgtcgcaa tacgtcgacg tcggcgccca
     2581 ggtctccggc cagctcatcg agctgccggt caagctaggc gataccgtga agcagggtca
     2641 actggtcggg gtcatcgatc cgacgccgta cacggccaag gtggcccagg atcgcgcgca
     2701 gattgccgac ctgcaggcgc aactggcgca gtcgcaggcg acgcttgcgc tcgcccgctg
     2761 gacttacgcg agcaacagca ccttggccgc gcaaggcgca gccaccggac aggcacgcga
     2821 gcaaagccag gcgaatctga aggtggcggt gaacgcggtg gcgtcgctgc aggcgcagat
     2881 cgacaaggcg aacaacatcc tcaaggtcga tcaggccaac cagggctaca cccggatcgt
     2941 ggcgcccatc gccgggctag tgatctcgcc cacgagcgcg aactacggcg ccgcctggaa
     3001 caagctggat atcgcacgac ggggtcagac tctcaacgcc aaccagaatg cccccgtgct
     3061 catgcgtatc gccaacatgg atcgcatggt ggtgcgcgcc caggtctccg aagccgacgt
     3121 tgcccggctc aagaccggca tgccagtcga tttcatcacc ctcggccggc ccgatcgtcg
     3181 tatcaaagcc cagctcggtg ccatcgaggc tacgccggaa ctggtcaacg gcgcgatctt
     3241 ctacgacgcc gttttcgagg tccccaacgc cgatcacagc ctgctgccgc agatgaccac
     3301 gcaggtgttc ttcgtggtgg cacaggccag caatgccttg atcgtgccgc tcacggcgct
     3361 gtcttcgatc cagcgccaga acggcgtggg tatgcccggt tgtcccgccg ctgcggcggt
     3421 cggaaatgcc gactgcgtcc aggtgctgat cgggggcaag ccggtggccc gaacggtgaa
     3481 ggtgggcgtg aaggacgagg tcaacgcaca gattctcgaa ggtctcgagg cgggcgatca
     3541 ggtcatcgtc ggcgcgaccg gcacgaccgc cgccagcggc agtggcagtg gcaccggcgg
     3601 cggcaagggc aaaggcaaag gagccggcag cgatggcggc gggcagtgaa ggcgcgcgcg
     3661 tgacgccgct gatcgaattg cgcgacgtcg tcaagcgcta tgcgcagggc gatctgagca
     3721 ttgaggtgct gcacggcatc tcgctcacga tccaggaagg cgaattcgtc gccatcatgg
     3781 gcgcgtcggg ttcgggcaag tccacgctga tgaacatcat cggctgcctg gaccgcccca
     3841 ccagcggtca ctaccgggtg ctgagtcgcg acgtcgccga actcgacagc gacgcgctgg
     3901 ccgagctgcg tcgcaacacc ttcggcttca tttttcagcg ctacaacctg ctaggcagtg
     3961 cgagcgctca ggaaaacgtc gaggttcccg ccctctatgc cggcctggca gccggaccac
     4021 gacgcgagcg agcgcgcctg ctgctgaccc ggctggggct cggtgagcgg ctggacaatc
     4081 ttcctggccg gctctcgggc gggcagcaac agcgggtgtc gatcgcgcgg gcgctgatga
     4141 acggcgggcg catcattctc gccgatgagc ccactggcgc cctggacagc cagagtggaa
     4201 aggaagtgat ggaactgctg cgcgaattgc atcgcgccgg ccacaccgtc gtgctcatca
     4261 cccacgaccc gaagatcgcc gaagaggccg accgcacggt gtgcgtcagc gacggccata
     4321 tcgtcgagga tacgggtgaa cgcgtcgccc gccgaaccga agctgtgttg ccagcgccca
     4381 gcgtgggtcc taccgggagc gtacgctcgg tgctgatgct ggacatcatg gcgtcggtgc
     4441 gcacggccct gcgcgcgctc agagccagcc gggtgcgcac cttcctgacc ctgctcgggg
     4501 tgatgatcgg tgtgggttcg gtggtggcca tgctcgcgtt tggcaacggc gctcgccagc
     4561 aggtgctgga tcagatccag gcgctaggca ctgatctgtt actggtcaaa cccggcgcac
     4621 ccggggtgcg tggggccggc ggcacgacaa cgctcgtgcc cgccgatgcc gaggcgctcg
     4681 tccggctgcc gaacgtgggc tacgtggtcc cgcagacatc caatccggtg acagtgcgct
     4741 acggcaacgc gacctacacc accaccctct acgcgaccac gcccgcattc accgcagcca
     4801 acaactggcc ggtggcgcgc ggcaccttca tcagtcaagc gaatctgcaa ggctttgcgc
     4861 cggttgcggt gttgggccag agtaccgcca aggctctgtt cagcgcggct gatccggtcg
     4921 gacgctatgt cctggtcaac aacacgccct tgcgcgtcat cggcgtgctg gcggcgaaag
     4981 gcgccgacca gcagggcaac gaccgcgacg acgccgtgtt cgtgccgctc acgacggggc
     5041 aggcgcgcat caccgggcag agctacctca aattcatcac catccagatc gacgacaccc
     5101 agcagatcgc cgctaccgag gcggcgatca acgcattgct gatcaagcgc caccgcaccc
     5161 aggatttcca ggtgcgcagc atgaccgcac tgctggagca gcaggcgcag acgcagcagt
     5221 cgctgacgct gatgctgggc gtggtcgcac tcatttcact gctggtcggc ggcatcgggg
     5281 tcatgaacat catgctggtg agcgtcaccg aacgcacccg cgagatcggg gtgcggatgg
     5341 ccgtcggcgc acgcaccttc cacatcctgc tgcagttcgt caccgaggcg ctggtggtgt
     5401 gcaccatcgg cggtctgatc ggcgtggcag gcggcttgct cgtcgcggga gtggcggcgt
     5461 ggagcggcgc cgcggtgctg gtcacgctgc cgccggtgct gcttgcattc accagcgctc
     5521 tcgccaccgg actggtgttt ggctacctgc cggccaaacg cgccgccgcg ctggatcccg
     5581 tcgtcgccct cagcacggag taaccatgat tgaccttctt aacatgcaga gatggagcgg
     5641 cctgatcgcc gccttgttgc tcgccggctg cgcggtcggg ccgacctatg agcgcccggc
     5701 agtcgaatcg ccgagcgact gggtggctga caaggccgat gcccgctggc ccgcgatcga
     5761 ctggtggacc gctttccgtg acccggtact cgaccggctg gtaatcgagg ccctggccaa
     5821 caatcacgat ctcaaggccg cggtgcaacg gatcgcccaa gcgcgggcac tggcccaagt
     5881 cgccggcgcg gctcactacc cgagcttgtc agccagtgcc caggccagtc ggcaaaacaa
     5941 ttccaacaac aatctcaacg gaaaattcgt tacgcccaac agttaccagg cgggactcac
     6001 cgcgagctac gtgctcgacc tgttcggcct gaatcgcgac caggccgagg cggcagctgc
     6061 gaagctgaag ggtagcgagt tcgagcgcca aaccgtcgaa ctcggcgtgg cggcggtcac
     6121 ggcgagcagc tacttcaaat tcgccgcgct cgacgcgcga ctcgcagtcg cgaaggagac
     6181 gttggccaag gcccgccatg cagctgaact cgtgcgcaca cggcagcaag ccggcatggc
     6241 gacggcactc caggtcgctc aacagcaagc cgaagtcgcc tcgctccagg cagcaattcc
     6301 gacgctccaa acacagcgcc gccaggtcct caatgcgctc gccatgcttg ccggacatct
     6361 gcccgatgga tttgatgtca agccgaccgc gattgcacaa cttggtctgc cccaagcccc
     6421 ggccggcctg ccgtccgcgc tgctggagcg ccgtccggac atcctgcgcg cggaggcggc
     6481 gctggcgggc gccaatgccg acatacgcgc cgccactgcc gcgctgttcc cgcgcatcaa
     6541 cctcaccgcc caaggcggct atgccagcgc tgccttgcgc caactcttcg atcccggcaa
     6601 cacgttttac agtttggcgg cagggctgac agcgcctctc ttcgaaggcg ggaaattgcg
     6661 aggcgagctc gcttacaccg aggctcgtta cggcgagctc gtgcagaatt atcgtcaaac
     6721 cgtactcgcg gccttcaccg acgtggaaaa cgctctgacg gcccagcgca atacggacga
     6781 cacgctagtt gcacaacgtg acgccgtcac tcaggccacg ctcgcctact cgctcgccga
     6841 attccagtac gagcagggta tggtcgatta tctggcggtg ctcaccgcgc agcgcagcct
     6901 gctcgctgca gacgacgccg aggctcaggc gcgatttgca cgccttgacg ctgcagtgtc
     6961 tgtctatcag gcgctggggg gcggctggaa catcccccaa tgaatgaaga cttggctgag
     7021 gccaaacggt gaacccgtgg ggttcttgct caattgggca accacacctt atagctgctt
     7081 tttctgacga aatgtgcggg tatggctcac gccaaagcgc ataaacatgg aactacgaca
     7141 cctccgctat ttcatcgccg tggcagaaga actccacttc gcccgcgcgg ccgaacgcct
     7201 gcacatcgag cagtcgccgc tatcgcgcgc catcaaggac ctggaatacg acttgggcgt
     7261 acaactgttc gagcgcacta cgaggagcac ccggttgaca tgggctggac aggtattcct
     7321 ggacgaggcg cggcgggtgc tatccacctt ggaacaggcc aaggccagtg ccaaggctgc
     7381 ggccactggc tatcgtggca cgctgcgcat cgcgctgtcc gatggtatcg ccccgccacg
     7441 actcgccgct ctgctcgcgc aaaatcgcga agaagagccg gaggtggaaa tccgtttgtt
     7501 cgaaacgacg ctggctcagc agttgaaagg gctatgcagc ggcctgtacg acgccggatt
     7561 cgcccatgcg gccgaggtcg gcgaagacat catcgccgaa ccggtgtgga ccgatccgct
     7621 ggtgattgct gttcccgcgc ggcatccgct gctggcgcat aagcgcattc ccttggagga
     7681 gttgatccgc tacccgctgg tgctatgtca cccggaagcc tgcgaaggct gtcacaggca
     7741 actcgaacgg atattgcgca ccgcgaatgt ggagcccatc gtggcggagc acgttaccac
     7801 cttcaatctg atgatgattt tggtagctgc gggctacggg ctgggattcg ccaacgaatc
     7861 gcagatgacg gtctgccgtc acccggaggt tgtggctcgc ccactggcgg ggcgagccct
     7921 catgctgagc acctacctgc tgcgtcccaa tacggaatcc tccgaaccgt tgagccgctt
     7981 catcgcccga gcgcttgaga caaccgtcag tcccgcaatc accaaatgaa agatcatgcg
     8041 atgaaaacat tccctgcgtt gttgcttgcc atcgtgctaa ctgcctgcgg tcaatctgct
     8101 cccaccgata ccgtcgattc gttagcggcc aaccccgaac gattgaagga actgcgccag
     8161 cagtgcaaag ccgaccgcgc gaaactgggc gacgcgttgt gcaacgcagt ggccgaagcc
     8221 acacgcaaac gcttcatggg caacggcacg ccgtacacgc ccgagtcggc cccaaagaac
     8281 taatttcacg caggtagcga cactgcggcc agtcaatgcc cgccgtatgg ctcggtacgt
     8341 gccgatccct cacgtcggca aaaaatcttc caacaaatca ccctacacgc cgcagcccac
     8401 tgttctttgc agcgatgcgc tccgcttttc cccagctaga aatcttgttt tcttcgtctc
     8461 cgctatcccg ataacggtct ttgaccggca ctgagttgac accgaacctc acgtctgcag
     8521 cacgtctttg tgcgcgcgat gtgcagaaga tattcggagg tcgagatgca agggaccagt
     8581 gtgctgttcg gtcaggtgtt gacggtgttc tgcatcgtca tcgccggtgt gtggggtgcc
     8641 acgcaatgga ctgccgccgc actgggctat cagctgcgcc tgggtactcc ctggttcgac
     8701 ttcttcggca cgtcgatcta tcacccgtgg aggctgttcg agtggtggtt tttcttcgac
     8761 gcttacgcgc cgcacatctt caacacgggt ggtgccatcg ccggtgccag tggcctggtc
     8821 gctatggtgg ttgccatcgg catgtcgatc tggcgctcgc ggcagtccaa gctggtcacg
     8881 acctacggct ccgcacgctg ggccgatgcc aaagaaatcc gcaaggccgg actgaccggt
     8941 ccggccggtg tctttctcgg cctgcatgac agccagtatc tgcgccatga agggccggaa
     9001 cacgtcctga ccttcgcgcc gacacgctcg ggcaaaggcg tgggtctggt agtgccgacg
     9061 ctgttgtcgt ggcccgcgtc cgccgtcatc catgacatca agggcgagaa ctggaacatc
     9121 accgctggct ggcgctcgcg cttctcacac tgcctgctgt tcaacccgac tgatcccaga
     9181 tcggctgcat acaacccgct gctggaagtc cgtcgcggcg cgcatgaagt gcgtgacgta
     9241 caaaacatcg ccgacatcct ggtcgatccc gaaggcgcac tggagcggcg caaccattgg
     9301 gagaagacat cgcacgcgct gttggtcggc gcgattctgc atgtgctcta tgcgggcaaa
     9361 gataagacgc tgcgcggtgt cgctaacttt ctatccgatc ctgcgtgccc gttcgaggtg
     9421 accttgcacc ggatgatgag cacgcggcac ctgggccaga gcacccatcc tgtcgttgca
     9481 tcggccgcgc gagaagtgct caacaagagc gacaacgaac gctcgggcgt gctgtccacg
     9541 gccatgagtt ttctgggtct gtaccgcgac cctactgtgg ctgaagtgac ttcgcgctgc
     9601 gactggcgca ttgccgacct gatctctgcc gaacatccgg tgtcgctgta cctggtggtg
     9661 cctccttcgg acatcagccg caccaagccg ctgatccgtc tgatcctcaa ccagatcggt
     9721 cggcggctca ccgagtcgct cgacggttcc gatggtgtgg agcgcaagca caagctgctg
     9781 ctgatgctcg atgagttccc ggcgctcggg cggctggact tctttgaatc ggcactggcc
     9841 ttcatggccg gctacggtct gcgtgccttc ctgatttcgc agtcgctcaa tcagatcgac
     9901 aaagcctatg gccagaacca ttcgattctg gacaactgcc atgtgcgggt aaccttcgcc
     9961 accaacgacg aacgcaccgc caaacgtatc tccgagacgc tgggcactgc taccgaattg
    10021 cgggcgcagc gcaactatgc gggccatcgt ctggcaccgt ggctggggca cctgatggtg
    10081 tcgcgccagg aaacggcgcg gcccctgttg acacccggcg aagtcatgca actgccgccg
    10141 gatgaatcgg tggtgatggt ctccggccat gccccgatca aggccaagaa gctgcgctac
    10201 taccttgatg ccaatttcaa gcggcgcgta ctgccgccgc ccacgctgac cttcggccgt
    10261 tatgccgacg tgccagcggc acgcgccgac gactggagcg ccttggctgt gcccactgtg
    10321 ccagccactt cgacgacggt tgggcttggc gagcaggccg atgccggcgg cccacgccag
    10381 cagccggagc tatccgagat tgccacctac agccccgaac ccgaacaacc ggccagtgac
    10441 ctgtcgctgc tcgatgacga cgatttctcg cttcctctcc catcccagct cgaccctcgc
    10501 ttgcagcgca cggcacggct ggccgccctc gaccctgacg acggaattca actatgagcc
    10561 aagcccgcct gaatctcttt atccaacatg accacgccaa gcgcctggac gaactggccg
    10621 caaagaaagg cgtgtccaaa tcctccatcg tcgcggcggc gctcgcgtcc tggctctcac
    10681 cggacgcggg tgaccaacgc gaggcggcca ctgccaaacg gttggatcgg ctgtcgcgcc
    10741 agttcgagcg tctggagcgt gaccagaaca tcctgatcga gacgctggcg ctgtacgtgc
    10801 gctactacct cacggtcagc acgccggtgc cggaggccca tcaagaagcg gccaaggctc
    10861 aaggcaaggc gcgcttcgag cagttcatcg aacagctcgg ccgccatgtg ctgcgcgggc
    10921 gcagcctggt caaggaggtc tacgaagaaa tccagcctga tgccactcgg ctggcagaag
    10981 cggccatgca ggaggacgga gcatgagcgc cgtcccgaag ttgccacccg agttgcagct
    11041 taagccgcca tcatcggccg ccacatcgtt ggaccggcgt atccgcatgc tgcgcacggc
    11101 gatggggccg ctgattgccg ccgcgctgga agacccggac gtggtggaga tcatgctcaa
    11161 tccagacggc tccttgtgga ttgatcgcct gtcgtcgggc cgctcgccga caggcgtcaa
    11221 gctgtccgaa gccgatggcg aacgcatcat ccggctagtc gccgcgcatg tcggcaccga
    11281 ggtgcatcgc ggccagccgt tgctgaccgc cgagctgccg gaaacggggg agcgcttcga
    11341 gggcatcctg ccgcccgctg cgccagggcc ggccttcgcc ttgcgcaagc gcgcagtgag
    11401 catcatcggc ctggaacgct acgtcgccga cggcatcctg accgaggaac aagccgagtt
    11461 cttgcgccgt gcggtgcgcg agcggctgaa catcctgatc gccgggggca cgagtaccgg
    11521 caagaccacg ctggccaacg cactgttggc cgagattgcc gccaccggcg accgggtact
    11581 ggtgctcgaa gacaccgtgg agttgcagtg tgccgcccgc gaccacgtat cgctgcgcac
    11641 gcggtctggc gtggtgtcga tggccgagct agtgcgggcc acgatgcggc tgcggcccga
    11701 ccgcgtgatc gtcggtgagg tgcgcggcgg tgaagcgctg gacctcatca aggtctgggg
    11761 cactgggcat cccggcggca tcgccaccat ccacgccagt tccgcccacg gtgcgctgct
    11821 gcgcctggaa caactgatcc tcgaagtcgc tttgacgccg ccgcgcgcgc tgatcgccga
    11881 ggcggtgaat gtcattgtct tcatcgccgg gcgcggccgt gcccgtcatg tccagaccat
    11941 cgcccgcgtt gtcggttttg acggccacgg ctaccagctc gacgacgcgc tcgctccgcc
    12001 gcttccgacc atctcgctcc cttcctcaac cacccctgga gaactgccat gacgcacacc
    12061 gttttttcga cttcccgctt ttctgtaaat ccgcatctgc ggctgacgtt gcacgttgcc
    12121 gtgctcgccg tgctgatgtt atgcctagca acgactgccc acgcggccgg ttccagcatg
    12181 ccctgggaag ggccgctgca atccatcctt gaatcgatcc agggaccggt ggcccggatc
    12241 gtcgcggtca tcatcatcat ttccaccggc ctggccctgg ccttcggcga tacgtcgggt
    12301 ggctttcgca agctgatcca gatcgtgttc ggtctgtcca tcgcgttcgc cgcatcttcg
    12361 ttcttcctgt cgttcttctc gttctcgggc ggagcggtgg tcgcatgagt gccgccaacg
    12421 aattcgcggc gggtttcgcc agtggtttcg agattccgct gcatcgctcg ctgaccgaac
    12481 cgatcctact gggcggcgcg ccgcgcaccg tggcgattgc caacggcacg ctggccgctg
    12541 ccgttggttt gggcctgcaa ctgtggatac ccggcctggt gctgtggatc gtcggccatt
    12601 cgctggcggt ctggggtgcg cggctcgatc cgcagttcat ggtcgtgttc tcccggcaca
    12661 tcaaacaccg cccactgctg gacgtgtgag accgacaagg agaacacgcc atgatgaatc
    12721 tcgctgaata ccgcaagcgt cccgcgctgc tggccgactg gctgccctgg gccgggctgg
    12781 ttgcaccggg tgtggtgctg aacaaggatg gttcgttcca gcgcacggcg cgctttcgcg
    12841 gaccggacct agacagcgcc acgcagggtg aactgattgc cacgtcggca cgtttgaata
    12901 acgcgctgcg ccggttgggg tcaggctggg ccttgttcgt ggaggccgag cggcgtgccg
    12961 ccgccgacta cccgcactcg gagtttcccg aaccactatc ctggctggtg gacgaagagc
    13021 gccgggcgtc gttcgaggaa gaccacagcc actttgagag tagctaccac ctgacgctcg
    13081 tgtacctgcc cgctgaggaa tcacgtgccc gcgcggccaa gctgctgtac gagaacacac
    13141 cgcaaggcgg cgtggactgg cgcgagcggc tggaatcgtt tatttcggag acggatcgct
    13201 tcttcgacct gcttgatggc gtcatgccgg agatcgtctg gctggacgac agccagacgc
    13261 tgacctatct gcatgcgacg gtctcgacgc ggcactatcc ggtcgctgtg ccagagcatc
    13321 cgttctacct cgatgccttg ctggccgatt ccgcgttgtt gggaggtttg gcacccatgc
    13381 tgggcgatca gcatctgcgc gtggcatcgg tgcgcggctt cccaacctcg acctggccgg
    13441 ggctgctgga cgacttgaac cgcctgggct tcgcctaccg ctggtccaca cgctttctct
    13501 gtatggacaa atccgaagcg gagaaggaac tcacacgcct gcgccgccag tggttcgcca
    13561 agcgcaagaa cgtggtcgcg ctgctgcgcg aaaccatctt ccagcaggaa agcccgctgg
    13621 tcgataccga tgcctccaac aagtcagcgg atgccgatgc ggccctgcag gagctgggca
    13681 gcgatcaagt ttccttcggc tatgtcaccg ttacggtgac ggtactcgat gccgatgccg
    13741 gcgcggctga cgaaaaattg cgtcggattg aacgtgtcat ccagggccgc ggtttcgtca
    13801 cgatcccgga aacgctcaat gccgtggagg cgtggctgtc ctcgattccg ggcaatgcct
    13861 acgccaatgt gcgacagccc atcatctcga cgctgaacct ggcgcacctg atgcccgtgt
    13921 ctgctgtatg ggccgggccg gagcgcaatg accacctgga cgggccgcca ttgatcgtga
    13981 cgcgcaccga cggtgccacg cctttccggc tggtgacgca catcggcgac gtgggccaca
    14041 cgctggtagt cggaccgact ggcatgggca agtcggtgct gctggcggcc ttggcaatgc
    14101 agttccgccg ctatcccggc tcacgcatct ttgccttcga catggggcgc tcgatgcgcg
    14161 ccaccatcct gggcctgggc ggcgagcact atgacctggg cacggacggc gaaatcgcct
    14221 tccagccgct ggcccgcatc gaccaggagg gctaccgcac ctgggccgcc gagtggctgg
    14281 aaggtcgctt gctgcacgaa ggcgcagccg tcggcccgga agaaaaggcc gcagtctggt
    14341 cagcgctcgg gagtcttgcg ggtgcgccgg tggagcagcg cacgctgacc gggctgtcgg
    14401 tgctgctgca atcgaatgcg ctgcgccagg ccttgcagcc ttacgtgctt ggcggcgcgc
    14461 acggcaagct gctggacgcg gattcagacc gtctggggtc tggctccgtc caatgcttcg
    14521 agatggaaga gctgatgcac agcaaggccg ccgtgctggc agtgctgggc tatctgttcg
    14581 cgcgcttcga cgaacgtttc gacggcgcgc cgaccttgct gattcttgac gaggcctggc
    14641 tgttcctcga cgacccggtg tttgccgcgc gtatccgtca gtggctcaag acgctgcgca
    14701 agaagaacgt cagtgtcatc ttcgccacgc agtcactggc cgacatccag aactcgtcca
    14761 tcgccccggc catcgtggag agctgcgcca gccgcatctt cctgccaaat ccgcaggcga
    14821 ccgaaccgca gattcgcacg atctacgaag gctttggttt gagccgtcgc cagatcgaca
    14881 tcgtggccga ggcggttccc aaacgcgact actactacca gtcccggctg ggcaatcgct
    14941 tgttcgacct ggacctcggg ccggtcacgc tggcctttgc cggtgcctcg acaccgcagg
    15001 accagcgcgc gatcgacgaa atacgtgatg cggtatcccg ttcggtgccg tctcccgact
    15061 tcgcagccgc ctggctgcgc catcgcggcc tcgattgggc cgccgacctt gttccgtcct
    15121 ttccgtccca ttcatccatc ccacaggagt acccatcatg aaaaaaacca tcctcgccat
    15181 tgccgccgca gcgctggtga ctctcatgcc taccgcacac gcgcagtggg tcgtgatcga
    15241 tccgtccaac ctggcccaga acatcatgac ggccgcacgg acgctggagc agatcaacaa
    15301 ccagatcagg cagctccaaa atcaggcgca gtcgctgatg aaccaggcga agaacctcac
    15361 cggtctggac ttcagcgcgt tgaatgaact gcgtgcttcg ctgtcggcga caaaccagct
    15421 tatccagcag gcgcagggat tggcgttcaa cgtgtcgcag atggagaccg aattccagcg
    15481 gctctacccc gacgcgtatt ccgcttccac ctcgggaacc cagatggcga ccgatgcgcg
    15541 ttcgcgttgg cgcaattcgc tcgaagcgct acgcaccgcg accaaggtgc agtcacaggc
    15601 ggtgcagaac ttcgcgtccg atgagcagac gctgacagac ctggtgaacc gcagccagtc
    15661 ggcggtcggc gctttgcagg ccacacaggc gaccaaccag ttgctggcct tgcaggcccg
    15721 gcagtcgatc caggcgcagc aacttcagat tacgcaagac cgtgcgaccg cgctggagca
    15781 ggcgcgcatt gttgccgtgc aggagcgtgc ccgcgaagtg cggcggcgct tcatcggtga
    15841 cggcacgccg tacaccccac agaccgttga tttctacggc aactgagggg acgccatgaa
    15901 cgacgtttcc gtcatcgacc atttcctcag cgtcttctcg cactacatcg actctggttt
    15961 cggtttgctg cgaggagaag ttgctttcct gacgaccacg ctggtggtga tcgacatgac
    16021 gctggccggc ctgttctggg ccatgagcca cgccagcggt ggcggcgatg acatcattgg
    16081 caaactcatc aagaaggtgc tgtatgtggg caccttcgcc tacatcatcg gcaatttcaa
    16141 tctgctggcc ggcatcgtgt tccggtcctt cgccggcttg ggattggtgg cgtcgggctc
    16201 aacgatcacg caagccgagt tcctgcagcc ggggcggctg gccaaggttg gcatcgacgc
    16261 gggtgcgccg atcttgaagc agatcggcga catggcgggc ttccccgaag tgttcgtgaa
    16321 ccttgacgcg atcgtcgtgc tgttcctggc ctggctggtg ctgattgcga gtttcttcgt
    16381 actcgcggtg cagctcttcg tcaccttgat cgaattcaag ctgaccacgc ttgcaggctt
    16441 cgtgctggtg ccgttcgcgc tgtggaacaa gacctcgttt ctcgctgaaa aggtgttggg
    16501 caacgtggtg tcgtcgggca tcaaggtgct ggtgctggcg gtcatcgtcg gcatcggaac
    16561 agggttgttt gccgaattcc aggtgacgcc gaatgaaccc tccatcgacc atgcgctgac
    16621 catcatgctg gccgcactgt ccatgctggg cctggggatc ttcgggccag gcatcgcgac
    16681 cgggctggtg tctggggcac cgcagttggg tgcaggtgcc gttgccggaa ccgcgctggg
    16741 cgttgcggga gcagcggtcg ccgtgggtgc tgccgcctcc ggcgtgggcg gcgcggtggc
    16801 ggccggtgcg cgcatggcgc cgggtgccgc aaagatggcc gcgagcggtg ccagctcgat
    16861 ggcctcgacc gccagcagcg cgaagtccgc gttccaggcc ggttctgccg gtgccggtgg
    16921 cgggctcaag ggtgcggcgg caggattggg caacgtcgcc aagacgggtg cgcaatcggt
    16981 cgggcagaag gtcgccgctg gagccaagtc gatgaaagac cgggctgcgg cggccatcaa
    17041 gccggatgcc ccggcatccg gggctggcgc tggtgctggt tcagccagta cagccagtgg
    17101 cccggccacc caaggcggca acgatgccgc gcccgccgat acccagcaac ccgcgtgggc
    17161 caagagcctg caccgtcgcc agcagatcag ccatgccgcc tcgaccgtcg cccacacgct
    17221 gcgcggtggg gacggcggcg gctcgggcag tagtcccagt ctgcgcgacg cctcggattc
    17281 ataaggagaa catccatgcg attcaaacgt tcattggtgc gctatgcgga cacgccgcag
    17341 cctgccaccc cgtaccaatc cgccgagcag gtctgggatg accgcatcgg ttcatctcgc
    17401 gtgcaagcaa agaactggcg gctgatggcc ttcggttgtt tggtactggc tctgctgatg
    17461 gctggcggcc tggtctggcg ctctgcgcag tccatcgtca caccctttgt ggtggaggtg
    17521 gacagtggcg ggcaggtgcg cgctgtcggc gaggccgcca cgccgtacaa gccgaacgat
    17581 gcgcagacgg cccatcacct ggggcgcttc gtcacgctgg tgcgctcact gtccatcgac
    17641 cccatcgtcg tgcggcagaa ctggctcgac gcctacgact acaccactga ccgcggcgcg
    17701 gcggtgctca acgactacgc acgcaccaac gatccctttg cccgcatcgg caaagagtcg
    17761 gtgacggtgc agatcaccag cattgtgcgc gccagtgacg cgtcgttcaa cgtgcgctgg
    17821 accgagcgtc gctatgtcaa cggcgcagct gctggactgg agcgctggac ggctgttgtg
    17881 tctgtcgtcc tgcaaacgcc gcgcaccgag gaacggttac gccgcaatcc gctgggcatc
    17941 tacgtcaatg ggttgtcgtg gagccgtgaa ctcgattctt ccgaaggagc caaaccatga
    18001 aaccgacttt gcgcatttac gtatttctgt cactgctggt cgccacatcc atgaccctct
    18061 cgggctgcgc cacgcagggc aagccgccgc cgaccatcac gcttgatgaa gcagtcgcgg
    18121 cgcagccgct gccggagttg cccaagccca tcgaagtggt cgcggttccg caacctttgc
    18181 cattgcccga acagttgaag ctgttgccgg atgaactgga ggccaagacg gcacctgaat
    18241 cggccgacga gaaggtacgc gtcacgcgcg ccaatcagga ggcccgaatt gcaccgaccc
    18301 gcgagggcta cctcaatgcg atccaggtct ggccgttcac cgatggtgcg ctgtaccagg
    18361 tctatgccag tccgggccgg gtgacagtgg tttcgcttca acctggcgag gaactggtga
    18421 cggtcgccgc tggcgatacc gtgcgctgga tcgtgggtga cacgtccagc ggcgcggggg
    18481 ccggtctacg cgtgagcgtt ctggtcaagc ccacgcgcag tggtctcaaa accaatctgg
    18541 ttgtgacgac caaccggcgc acctatctga ttgagttgac ctcgacagag aaggcctgga
    18601 tggcatccgt gtcctgggac tatccgaagg accgcatgct ggccttgcag cgccaggcac
    18661 aggcggccca ggttgccgca ccggtcgatg cgggcctgtc gctggagaag atccggtttc
    18721 gctacgcgat caccggcagc aatccaccct ggaagccgct gcgcgccttc gacgatggcg
    18781 agaaggtcta tatccagttc cccagcggca ttgcgcaggg cgagttgccg ccgctgttcg
    18841 tcatcggcgc gcaggggggc ggccaactcg tgaactaccg tttccgttcg ccgtactaca
    18901 tcgtggatcg gttgttcggc gcggccgaac ttcgcttggg cggcgggagt ggtgacaaag
    18961 gaggcgacgt ggtgcggatc gagcgcacgg atgggattgt gagcgggacg cggaggaact
    19021 gaccatgagc caagcgaaca atgcacctga cactccggtg ccagacgtgc cgcccaaggt
    19081 ggccccggaa aacgtgacgc tgcgctccca gccgcgtccg gtcacgcgcc tgaaccgacg
    19141 catgctggcc gttctcgctg gagggctggc gaccgccgtg ctcggtggca ccatctggtc
    19201 gttgcaatca acgcagcgcc gtggcgctgc cgaccaaaag gagttgtaca acgtcgatcg
    19261 tgtgtcgcgc tcggaagggc ttgatcgact gccagcagat tattccaagc tgccgccgac
    19321 tttgccaacc gcagtgccgc aactcggtgc gccgctgcca ggcgacctgg gcgggccgat
    19381 ccacaaggcc gagcaacaag cgcaggggta tggctatcag cagcctggcc atgacccggc
    19441 cgaggttgaa cgcctggcgc gtttgaagca ggccgaggaa gcggcggctt cgtcgatgtt
    19501 cttccgttca agtggtcaga aggttgccgc gccgacgcag actgcgcctg ccgcccaagt
    19561 ggcggcgaac gccgcc
//
LOCUS       DASRUM010000059         3833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1707728, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000059 DASRUM010000000
VERSION     DASRUM010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707728"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..131
                     /locus_tag="VFF22_04355"
     tRNA            47..131
                     /locus_tag="VFF22_04355"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:81..83,aa:Leu,seq:caa)
     gene            504..716
                     /locus_tag="VFF22_04360"
     CDS             504..716
                     /locus_tag="VFF22_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZX16088.1"
                     /translation="MSNRQSGTVKWFNDEKGFGFITPESGPDLFVHFRAIQSNGFKSL
                     KEGQKVSFIAVQGQKGMQADEVQIQE"
     gene            910..1404
                     /locus_tag="VFF22_04365"
     CDS             910..1404
                     /locus_tag="VFF22_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZX16089.1"
                     /translation="MPKHLLRPQGEFPSAGLTRRLAAMFYDFLLCIALLIVVTFIYKL
                     ILMGFYGEAQLKQMSDAGALDGDPLLSTLLFLSLFGFFAKFWTHNGQTLGMQVWGLRI
                     QNADGTAISLWQALLRFLVAIGSWLLLGLGFLWMLWDKQKRSWHDIYSDSQVIQLPKN
                     IHKK"
     gene            complement(1466..2527)
                     /gene="lptG"
                     /locus_tag="VFF22_04370"
     CDS             complement(1466..2527)
                     /gene="lptG"
                     /locus_tag="VFF22_04370"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HZX16090.1"
                     /translation="MVKLDRYIGAQVFVAILAVLGIIVGLALLFAFIDELGDVEDNYS
                     LLDALGYVLLTAPRRIYEMLPMAALIGCLIGLGSLASNSELTIMRAAGVSIGRIVWAV
                     MKPMLVLMLVGVLIGEYLAPYSENQAQASRAMAQGGGEAQSAKRGLWHRQGQEYVHIN
                     AVQPNGVLFGVTRYRFDEQKRMQSSSFARRAQYQGDHWQLEDVATTHFRERSSEVITQ
                     GSERWDVELTPQLLGTVVLAPDALSMTGLWNYIHYLAEQGLNNSQYWLSFWTKVLQPL
                     VTAALVLMAISFIFGPLRSVTLGQRVFTGVLVGFVFKISQDLLGPSSLVFGFSPLLAV
                     LIPAGICALAGVWLLRRAG"
     gene            complement(2520..3641)
                     /gene="lptF"
                     /locus_tag="VFF22_04375"
     CDS             complement(2520..3641)
                     /gene="lptF"
                     /locus_tag="VFF22_04375"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HZX16091.1"
                     /translation="MIVFRYLSREVLLTLSAVSAVLLVIIMSGRFIRYLAQAAQGLLD
                     PGVLLMIMAYRIPGFLQLILPLALFLGILLAYGRMYLDSEMTVLSATGMSQQRLLTYS
                     LAPAALVALLAGWLSLGLAPQGVEHVARILNQQSAMTELDTLVPGRFQSMKNGSRVTY
                     TEELSEDRGSLAGVFISEKQLSREGDKERGISVLVAESGRQEIQADGSRYLILENGYR
                     YDGNPGQADYRAIKYDTYGVLLPKPEVAVEVTDREAMPTRELIGSDKPRMQSELQWRL
                     SIPVLVFVVTLLAVPLSRVNPRQGRFLKLLPAILLYMAYLALLIGARGALDKEKIPAA
                     LGLWWVHGVFVLIGLLLLYWEPLRLRWAARRVAQEVARG"
BASE COUNT          905 a         1205 c         1006 g          717 t
ORIGIN      
        1 tggattgacc aaccccaaca ccatccctat actgcgccgc cattgtgccc cggtggcgga
       61 accggtagac gcggcggatt caaaatccgt tttcgaaaga agtgggagtt cgagtctccc
      121 ccggggcacc atagattcga agaaaggcct tgagattcaa ggcctttttt attgcctgaa
      181 ataaagctac gacacacccg aacagcactc cgatatcgcg ctgactaatg cgccagactc
      241 ccgcaaagcg catgctccta gggccgcgca aaacacctac aacccaacaa ccgcatgccc
      301 tgcaggggct tggcatttgc ctgggagttg gcgtaaagtt ttcccactgt gtttgcatgg
      361 gtcgcgaaaa tcgtgacctg gtgcagtaga tcatctagat ccgctacatc ccgctcgacc
      421 gtcgcacacc gccttgcaac cagtattcag cccctcatgt cgagtagctg ctgtcatcaa
      481 cttcaggttc aaggaaatac aacatgtcga atcgtcagag cggtaccgtt aagtggttta
      541 acgatgaaaa aggttttggt tttatcactc ctgaaagcgg tccggatctg ttcgtgcact
      601 tccgcgcgat ccaaagcaac ggtttcaaga gcctgaaaga aggccagaaa gtcagtttta
      661 ttgctgtgca aggccagaaa ggcatgcagg ctgacgaagt acaaattcag gaataatcct
      721 gccgtgctga aaaacccctg atggcaacat caggggtttt ttatgccctg ccattcatgg
      781 cggccaccct ttgggccgtc gctgcgagat gttaaaaatg actcccggcc atttttttat
      841 ggccgaccat tcctatgaat gccgtattgt tgccgccatt ccgccagccg attgagtaaa
      901 gagccgacca tgccgaaaca cctgctgcgc ccgcagggtg aattccccag cgccggactg
      961 acccgccgcc tcgcggcgat gttttatgac tttctgctgt gcatcgcact gctgatcgtc
     1021 gtgaccttta tctacaagct gatcctgatg gggttctacg gcgaggcgca gctcaagcag
     1081 atgtccgacg ccggcgccct ggatggcgat cctctgctct cgactctgct gtttctcagc
     1141 ctgtttggct tcttcgccaa gttctggacc cacaacggcc agaccctggg catgcaggtc
     1201 tggggcctac gcatccagaa tgccgacggc actgcgatca gcctatggca ggcattactg
     1261 cgctttctgg tagccatcgg ttcctggctg ctgcttggcc tgggctttct ctggatgctc
     1321 tgggacaagc aaaagcgcag ctggcatgac atctattcgg acagccaagt gattcagctg
     1381 ccaaagaaca tccataagaa gtaaattcaa tgcataaaaa agccggcgtg cacccagtgt
     1441 gcacgccggc ttttttacgc ctgaatcaac ccgcgcggcg tagcaaccag acgccggcca
     1501 gcgcgcagat acccgcaggg atcagcaccg ccagcaacgg cgagaagccg aataccagac
     1561 tcgacggacc gagcaggtcc tggctgatct tgaacacaaa gcccaccagc accccggtaa
     1621 acacccgctg ccccagggtt accgagcgca gcgggccgaa gataaaggag atagccatca
     1681 acaccagcgc cgccgttacc aacggctgta gcaccttggt ccagaacgac agccagtact
     1741 ggctgttatt cagcccttgc tcggccaggt aatggatgta attccacagc ccggtcatcg
     1801 acagcgcatc cggcgccagc accacggtac caagcagctg cggcgtcaat tcaacatccc
     1861 agcgctcgct gccctgcgta ataacctcac tgctgcgctc acggaaatgc gtggtcgcga
     1921 catcttccag ctgccagtga tcgccctggt actgagcacg tcgcgcgaag ctggaagact
     1981 gcatgcgttt ctgttcatcg aagcgatagc gagtaacgcc gaacagcacg ccattgggct
     2041 gcacggcgtt gatgtgcaca tattcctgac cctgacgatg ccacaggccg cgcttagcgc
     2101 tttgcgcttc accgccgccc tgggccatgg cacgactggc ctgggcctga ttttcgctat
     2161 agggcgcgag atactcaccg atcaacacgc cgaccagcat cagcaccagc atcggcttca
     2221 tcaccgccca gacaatccgc ccgatcgata caccggccgc acgcatgatg gtcagttcgc
     2281 tgttactggc taacgagccc aagccgatca ggcaaccgat cagcgccgcc atcggcagca
     2341 tttcatagat gcgccgcggc gccgtcagca gtacatagcc gagagcatcg agcaggctgt
     2401 agttgtcctc gacatcgccc agctcgtcga taaaggcaaa cagcagcgcc aggccaacaa
     2461 tgatgcccag cacggcaagg atggcgacaa atacctgcgc accgatgtaa cgatccaact
     2521 taaccacggg ccacctcctg tgccacacga cgggccgccc agcgcaaacg cagcggctcc
     2581 caataaagca gcagtagccc gatcaatacg aacacgccat gcacccacca caagccaagt
     2641 gctgcgggga ttttctcttt atccaaggcg ccacgcgcgc cgatcagcag cgccagataa
     2701 gccatgtaca gcagaatggc cggcaacagt ttgagaaagc gcccctggcg tgggttaacc
     2761 cgcgacagcg gcacagccag cagggtgacc acaaaaacca gcactgggat cgacaagcgc
     2821 cactgcagtt cggactgcat gcgcggtttg tcgctgccaa tcagctcgcg cgtcggcatc
     2881 gcctcgcgat cggtgacctc caccgctact tctggcttgg gtagcagcac gccataagtg
     2941 tcgtatttga tggcgcggta atcggcctga cccggattgc cgtcatagcg gtagccattt
     3001 tccagaatca gatagcgact gccatcggcc tggatctcct gacgaccgga ctcggccacc
     3061 agcacagaga taccacgctc cttgtcgccc tcgcgggaca gctgcttctc ggatataaac
     3121 accccggcca acgagccgcg atcctcggac agttcctcgg tgtaggtcac ccgcgagcca
     3181 tttttcatcg attgaaaacg cccaggcacc agggtatcca gctcagtcat ggcactctgc
     3241 tggttaagaa tccgcgccac atgctcaacg ccttgtggag ccaggcccag gctcaaccag
     3301 ccggccagta acgccaccag ggccgcaggc gccaggctgt aggtcagcaa acgctgctgg
     3361 ctcatgccgg tggccgagag cacagtcatc tcgctgtcca ggtacatccg cccgtaggcc
     3421 aggagaatgc cgaggaacag cgccaacggc agaatcagct ggaggaaacc gggaattcgg
     3481 tacgccatga tcatcagcag caccccaggg tcgagcagac cctgcgccgc ctgggccagg
     3541 taacggataa agcggccact cataatgatg accagcaaca ccgcgctcac ggcactcagg
     3601 gtcaacaaca cttcacgaga cagataacgg aagacgatca aaccagacac tccagggttg
     3661 tcaggctcag gcggctaaga tcaaactagc cgactgcttg ccggctcgcc caactggcga
     3721 accatcgaaa aatagccggc attatcctgc aataccgacg ccttgtcttg gggacaccac
     3781 gtaatggaat ttattgttaa aagcgcacgc ccagaaacac taaaaaccgc cac
//
LOCUS       DASRUM010000060        13573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1540480, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000060 DASRUM010000000
VERSION     DASRUM010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 13573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13573
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540480"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VFF22_04380"
     CDS             <1..802
                     /locus_tag="VFF22_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HZX16092.1"
                     /translation="VKKKTEKQTTSWIGEVEKYSRQIWLAGLGAYSKVSKDGNKLFDT
                     LVKDGEKAEKQAKTEVDKQVGAVKSSVGSTVGSAKSRVDEVKDKAMGKWGELEEAFDK
                     RLNNAISRLGVPSRNEVKALNAKVESLTKQIEQLTGVSVKSVKPAVKKVAAKPAAKPA
                     AKPVAKAAAKPAAKPAAKPAAKPVAKAAAKPAAKPAAKPVAKAAAKPAAKPAAKPAAK
                     PAAKPAVKAAAKPAPAKPVAAKPAAPVAAAAPAPAAAPAAPVTPATQS"
     gene            complement(866..1480)
                     /locus_tag="VFF22_04385"
     CDS             complement(866..1480)
                     /locus_tag="VFF22_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010169183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZX16093.1"
                     /translation="MKTRDRILECALLLFNQKGEPNVSTLEIANELGISPGNLYYHFH
                     GKEPLILGLFERFQAELAPLLSPPEDVQLDVEDYWLFLHLIVERLAHYRFLFQDLSNL
                     TSRLPKLERGMRHWLNSLKRTLAELLARLQLSGQLHSDTQSLGQLVEQITLTLLFSLD
                     YQRIIGREGESRLVVYQVMMLVAPHLVEESRYAAEHLAQRYLED"
     gene            complement(1568..3250)
                     /gene="phaC"
                     /locus_tag="VFF22_04390"
     CDS             complement(1568..3250)
                     /gene="phaC"
                     /locus_tag="VFF22_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HZX16094.1"
                     /translation="MRDQPAKRTLPVRSSVMNAQNAVVGLRGRDLLSTLRDLTLQGIK
                     QPIYSAKHVAALGGQLGRVLMGDKPFKVNPQDPRFADPTWRDNPIYSRSLQAYLACQK
                     QLNSWIDESNLDADDRARARFVATLLGDALAPSNTLLNPQVIKELFNTGGSSLLKGVT
                     HLFDDLVNNDGMPSQVSKHAFEVGRNLATTNGAVVFRSEMLELIQYKPMSEKQYAKPL
                     LIVPPQINKFYIFDLSSEKSFVQYALKNGLQTFVVSWRNPDPRHREWGLSSYVEALEV
                     AIDACREITGSKDVNLLGACAGGLTIAALQGHLQAKRQLRKVSSATYMVSLLDSQIDS
                     PAMLFADELTLEAAKRRSYQQGVLDGRDMARVFAWMRPNDLIWNYWINNYLLGKQPPA
                     FDILYWNNDNTRLPAALHGDLIDFFKHNPLTRPRGLEVCGTPVDLQKVTVDSFSVGGI
                     NDHITPWDAVYRSTLLLGGNRRFILSNSGHIQSILNPPGNPKSNYMENPKLTGDPRAW
                     YHDAEHVQGSWWPVWLSWMQERSGEQKDTLMALGNQNYPAMEAAPGTYVHVR"
     gene            complement(3455..4315)
                     /gene="phaZ"
                     /locus_tag="VFF22_04395"
     CDS             complement(3455..4315)
                     /gene="phaZ"
                     /locus_tag="VFF22_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006112 - energy reserve metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxyalkanoate) depolymerase"
                     /protein_id="HZX16095.1"
                     /translation="MPLPFVFRTIELDGQTIRTAVRPGNGQMTPLLIFNGIGANLELV
                     MPFVRALDPGLEVIAFDVPGVGGSSTPSTPYRFPGLAKLAARMLDYLDYGQVNAIGVS
                     WGGALAQQFAHDYPERCKKLILAATSAGAVMVPGKPKVLWCMASPRRYVQPSYGVQIA
                     PEIYGGAFRRDPKLALAHASKVRSGGKMGYYWQLFAGLGWTSIHWLHKIRQPTLVMAG
                     DDDPLIPLVNMRLLAWRIPNSELHVIDDGHLFLVTRAEAVAPIIMKFLEEERQRAIMH
                     PQPTPARTLS"
     gene            complement(4471..6150)
                     /gene="phaC"
                     /locus_tag="VFF22_04400"
                     /pseudo
     CDS             complement(4471..6150)
                     /gene="phaC"
                     /locus_tag="VFF22_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253743.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II poly(R)-hydroxyalkanoic acid synthase"
     gene            complement(6572..6946)
                     /locus_tag="VFF22_04405"
     CDS             complement(6572..6946)
                     /locus_tag="VFF22_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HZX16096.1"
                     /translation="MRMPTAIKLHKASKTLELHYGADERYTLPAEFLRVHSPSAEVQG
                     HGKPILQTGKLGVGLNKIEPAGNYALKLTFDDGHDSGLFTWDYLEQLAMRQAELWNDY
                     LATLSAAGKSRDPSESVVKLML"
     gene            complement(7004..8344)
                     /gene="hslU"
                     /locus_tag="VFF22_04410"
     CDS             complement(7004..8344)
                     /gene="hslU"
                     /locus_tag="VFF22_04410"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HZX16097.1"
                     /translation="MSMTPREIVHELNRHIIGQDDAKRAVAIALRNRWRRMQLPLELR
                     AEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGRDVESIIRDL
                     ADAAIKMLREQEMIKVRHRAEDAAEERILDALLPPARTGFSEEAAPGSDSNTRQLFRK
                     RLREGQLDDKDIDIEVAEAPAGIEIMTPPGMEEMTNQLQSLFSNMGKGKKKSRKLKVK
                     EALKMVRDEEAARLVNEDELKAQALEAVEQNGIVFIDEIDKVAKRGNTGGADVSREGV
                     QRDLLPLIEGCTVNTKLGMVKTDHILFIASGAFHLSKPSDLVPELQGRLPIRVELKAL
                     TPQDFERILTEPHASLTEQYAALLNTEGLGIQFVADGIKRIAEIAYQVNEKTENIGAR
                     RLHTLLERLLEEVSFSAGDLAGQQNGAPIMIDAAYVNSHLGELAQDEDLSRYIL"
     gene            complement(8372..8902)
                     /gene="hslV"
                     /locus_tag="VFF22_04415"
     CDS             complement(8372..8902)
                     /gene="hslV"
                     /locus_tag="VFF22_04415"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009682353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HZX16098.1"
                     /translation="MTTIVSVRRNGKVVMGGDGQVSLGNTVMKGNAKKVRRLYHGQVL
                     AGFAGATADAFTLFERFEGQLEKHQGHLVRAAVELAKDWRTDRSLSRLEAMLAVANKD
                     ASLIITGNGDVVEPEQGLIAMGSGGGFAQAAAMALLQKTDLSAREITETALNIAGSIC
                     VFTNQNLTIEEEDCAE"
     gene            complement(9188..9868)
                     /locus_tag="VFF22_04420"
     CDS             complement(9188..9868)
                     /locus_tag="VFF22_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZX16099.1"
                     /translation="MAAKKKPAAKRGASRYQAPAKKPVPGWFWLVCGLVIGGFMVFLF
                     SLEPGRDEIKRTKVEQARVTQANKAAAAKPASNEPAKPKYDFYTLLPESEVILPPQGI
                     EPPAPPAQKPVTPEEAAKIDAARAEAALNGQVPPPPPVVAKGPVSSQFFLQAGSFRRK
                     DDADSLRAQIILLGQSVQVESGTVREETWHRVLVGPFATREQLGQAQKSLAASGFSNL
                     LLQQRQAQ"
     gene            complement(9869..11608)
                     /gene="argS"
                     /locus_tag="VFF22_04425"
     CDS             complement(9869..11608)
                     /gene="argS"
                     /locus_tag="VFF22_04425"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZX16100.1"
                     /translation="MKDSIRHLIQQALTRLTADGVLPEGLSPAIQVENTKDKTHGDFA
                     SNIAMMLAKPAGLKPRDLAEKLIAALPSDAAISKAEIAGPGFLNFYQNTQALAARLDA
                     LLADAKLGVRKAGPAQRVVVDLSAPNLAKEMHVGHLRSTIIGDGVARVLEFLGDEVIR
                     QNHVGDWGTQFGMLLAFMQENPAAAESELADLEGFYRAAKKRFDESPEFAERARELVV
                     QLQAGDAECLRLWHRFNDISLSHCQALYDRLGVKLSMADVKGESAYNDDLANVVADLK
                     AKGLLSESDGAQCVFMDEFKNAEGNPLPLIVQKAGGGYLYATTDLAATRYRANVLKAD
                     RALYFVDQRQALHFQMVFTAARLAGFVPANMQLEHMGFGTMNGADGRPFKTRDGGTVK
                     LVDLLDEAEERAYALVKGKNPELDEAELRQIARAVGIGAVKYADLSKHRTSDYRFNFE
                     LMLSFEGNTAPYLLYAYTRVASVFRKLGKGFDEIGGNLDLQAAHEIELAGKLAQFAEV
                     LASVADKGEPHLLCAYLYDLAGLFSSFYENCPILSTEDEALRNSRLRLAALTARTLKQ
                     GLELLGLETLERM"
     gene            complement(11823..13511)
                     /gene="betA"
                     /locus_tag="VFF22_04430"
     CDS             complement(11823..13511)
                     /gene="betA"
                     /locus_tag="VFF22_04430"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HZX16101.1"
                     /translation="MPQEFDYIIIGAGSAGNVLATRLTEDADVSVLLLEAGGPDYRLD
                     FRTQMPAALAFPLQGRRYNWAYLTEPEPHMNNRRMECGRGKGLGGSSLINGMCYIRGN
                     AMDYDGWAEEKGLEDWSYLDCLPYFRKAETRDIGANAYHGGDGPVSVTTPKNGNNPLF
                     HAMIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPKGRRASTARGYLDQARERPNLTI
                     VTHALTDRILFSGKRASGVAYLLGDNDTPITVNARREVLLCGGAIASPQVLQRSGVGP
                     AALLRELDIPLVHDLPGVGQNLQDHLEVYLQYACKQPVSLYPALQWWNQPMIGANWLF
                     LGKGIGASNQFEAGGFIRSSAEFEWPNIQYHFLPVAINYNGSNAVKEHGFQAHMGSMR
                     SPSRGRIQVTSKDPRQHPSILFNYMAHEQDWREFRDGIRITREIMAQSALDPYRGREI
                     SPGAELQSDAELDAFIREHAETAYHPSCSCKMGEDDMAVVDGQGRVHGVQGLRVVDAS
                     IMPLIVTGNLNAPTIMMAEKIADKIRGRAPLPRSTAPYFVAGNAAARGTAQRSA"
BASE COUNT         2379 a         3956 c         4451 g         2787 t
ORIGIN      
        1 tgtcaaaaag aaaaccgaaa agcaaaccac ttcctggatc ggcgaagtag aaaaatactc
       61 gcgtcaaatc tggctggctg gtttgggtgc ctactccaag gtcagcaagg acggcaacaa
      121 gctgttcgac acgctggtca aagatggcga aaaagctgaa aagcaagcca agaccgaagt
      181 cgacaagcaa gttggcgccg tgaagtcctc tgtgggttcc accgtgggct ccgccaagtc
      241 gagagtcgat gaagtcaaag acaaggctat gggcaagtgg ggcgagctgg aagaagcctt
      301 cgacaagcgt ctgaacaatg ccatttcacg tctcggtgta ccgagccgca atgaagtgaa
      361 agcgctcaac gccaaggttg aaagcctgac caagcagatc gaacaactga ccggcgtttc
      421 ggtcaagtcg gtcaagcctg ccgtcaagaa agttgctgca aagcctgccg ccaaacccgc
      481 tgccaagccg gttgctaaag ctgcggctaa accagcggcc aaacctgctg ctaagcctgc
      541 cgctaaaccg gtagccaaag ctgcggccaa gccggctgct aaacccgctg ctaagccggt
      601 agccaaagct gccgctaagc cagcagctaa acctgctgcc aaaccagcag ccaagcctgc
      661 cgctaaaccg gcagtcaaag ctgcggccaa gccggcacca gccaaaccag tagccgctaa
      721 accggctgct ccggtggccg ctgctgcgcc tgctccggca gccgctcctg ctgcacctgt
      781 gacacctgct acccagtcgt aagacgcgca gcagtcaacg caaacgcccg gcctagtgcc
      841 gggcgtttgc gtttaggtgt tcgcgttaat cttccaggta acgctgtgcc aggtgttcgg
      901 cggcgtagcg tgactcttca accaggtgcg gagccaccag catcatcacc tgatacacca
      961 ccaggcgact ctcgccttcg cggccgatga tgcgttggta gtccagcgag aacagcaggg
     1021 tcagggtgat ctgctcgacc aactggccca gcgactgggt gtcgctgtgc agttggccac
     1081 tgagctgcag acgcgcaagc agttcggcca gggtgcgttt caggctattg agccagtggc
     1141 gcatgccgcg ttccagtttt ggcaggcggc tggtcaggtt ggacaggtcc tgaaacagga
     1201 agcggtagtg cgccaggcgc tcgacgatca agtgcaggaa gagccagtag tcttccacat
     1261 ccagttgcac gtcttcgggt ggtgagagca gcggcgccag ttcggcttga aagcgctcga
     1321 acagaccgag gatcagcggt tccttgccgt gaaagtggta gtacaggttg cctgggctga
     1381 tgcctagttc gttggcgatt tccagggtgg agacattcgg ttcgcccttc tggttgaaca
     1441 gcagcagggc acattcaagg attctgtcgc gggttttcat ccggtcttct tattctggcc
     1501 gcgggaaggc tgacgatacc tggccgcaat agcttgcggc cagtctgctc atgcttggct
     1561 tggcgtttta gcgcacatgc acataggtgc cgggcgcggc ttccatggcc gggtagttct
     1621 ggttgcccag ggccatcagg gtgtctttct gctcgcccga gcgctcctgc atccagctca
     1681 gccacaccgg ccaccagctg ccctgcacat gctcggcgtc gtggtaccag gcgcgtgggt
     1741 cgccagtgag tttggggttt tccatgtagt tggatttggg attgcccggc ggattgagaa
     1801 tgctctggat atgcccgctg ttggatagga tgaaacgtcg attgccgccc agcagcaggg
     1861 tcgagcggta caccgcatcc cagggggtga tgtgatcgtt gattccgccg acgctgaagc
     1921 tgtccaccgt gaccttctgc agatcgaccg gggtgccgca tacttccagg ccgcgcgggc
     1981 gggtcagcgg gttgtgcttg aagaagtcga tcaggtcgcc atgcagggca gctggtaagc
     2041 gagtgttgtc gttgttccag tagagaatat cgaaggccgg tggttgcttg ccgagcaggt
     2101 agttgttgat ccagtaattc cagatcagat cattcgggcg catccaggca aacacccgcg
     2161 ccatgtcgcg gccatccagc acgccttgct ggtaggagcg gcgtttggca gcctccaagg
     2221 tcagttcgtc ggcaaacagc atggccgggc tgtcgatctg gctgtcgagc aggctgacca
     2281 tgtaggtggc gctgcttact ttgcgcagct gccgtttggc ctgcagatgg ccctgcaggg
     2341 cagcgatggt caggccaccg gcgcaggcgc caagcaggtt gacgtccttg ctgccggtga
     2401 tttcccgaca ggcatcaata gcaacttcga gtgcttccac gtagctcgac aggccccact
     2461 cgcggtgccg cgggtcaggg ttgcgccagc tgaccacgaa ggtctgtagg ccgttcttca
     2521 gcgcgtactg aacgaagctc ttctcgctgg agaggtcgaa gatatagaac ttgttgattt
     2581 gcggcggcac gatcagcagc ggcttggcgt actgcttttc gctcatcggc ttgtattgga
     2641 tcagttctag catctcgctg cgaaacacca cggcgccgtt ggtcgtggcc agattacggc
     2701 cgacctcgaa ggcatgtttg ctgacctggc tgggcatgcc gtcgttgttc accaggtcat
     2761 cgaacaggtg cgtcacgccc ttgagcaggc tgctgccgcc ggtgttgaac agctccttga
     2821 tcacctgcgg gttgagcagg gtgttggacg gtgccagggc atcgcccaat agggtggcga
     2881 caaagcgtgc gcgggcacgg tcgtcggcat ccaggttgct ctcatcgatc cagctgttca
     2941 gctgtttctg gcaggccagg taggcttgca ggctacggct atagatcggg ttgtcgcgcc
     3001 aggtcgggtc ggcgaagcgt gggtcttgcg ggttgacctt gaacggcttg tcacccatca
     3061 acacacgacc cagttggccg cccagcgcgg cgacatgctt ggcactgtaa atgggttgtt
     3121 tgatgccttg cagggtcagg tcacgcaggg tggaaagcag gtcgcggcca cgcaggccga
     3181 ccactgcgtt ctgggcattc atcacgctgc tacgtacagg caaggtgcgt ttggctggtt
     3241 ggtctcgcat tgggcaacac tccgtcgtca aaccgtgaga cttcaaggtt gatagataag
     3301 cagcaaggct tataccaatg cgcgcgcatg ggcgttccag actgccgcgc gccaagtccc
     3361 aggtaataca acgtgcccga aaatgggtca agcattgtgc tttcagcaca gcctcagtgc
     3421 gcgctgttga ggtgcttgcc caaccaggga gcgtttagct gagcgtgcgg gctggcgtgg
     3481 gctgcgggtg catgatcgcg cgctgacgct cttcctcgag gaatttcatg atgatcggcg
     3541 ctacagcttc agcacgggtt accaggaaca ggtggccatc gtcgatcaca tgcaattccg
     3601 agttggggat acgccaggcc agcaagcgca tatttaccag cgggatcagc gggtcgtcgt
     3661 cgccagccat caccagtgtc ggttggcgga tcttgtgcag ccagtggatg ctggtccagc
     3721 cgaggccggc gaacagctgc cagtagtagc ccatcttgcc gcccgaacga actttactgg
     3781 catgcgccag ggccagtttc gggtcgcggc ggaacgcgcc gccgtagatt tccggggcaa
     3841 tctgcacgcc gtaggacggc tgcacatagc gccgtgggct ggccatgcac cagagtacct
     3901 tgggtttgcc aggcaccatc accgcgccgg ccgaggtggc cgcgaggatc agcttcttgc
     3961 agcgctctgg gtaatcgtgg gcgaattgct gtgccagggc gccgccccag gacacgccaa
     4021 tcgcattgac ctgaccgtag tcgaggtaat cgagcatgcg tgccgccagc ttggccaggc
     4081 caggaaagcg atatggcgtg ctgggcgtgg aggagccgcc gacgccgggg acgtcgaagg
     4141 cgatgacctc cagaccaggg tccagggcgc gtacaaaggg catgaccagc tccaggttgg
     4201 ccccgatgcc gttgaagatc agcagtgggg tcatctggcc gttaccgggg cgaaccgcgg
     4261 tacggatggt ctggccgtcc aattcaatgg tacggaatac gaatggtagc ggcatgcgca
     4321 aaagccctat caattaaaac gctggatcag accctccggg tcttttccag aaacaccagg
     4381 gcaaagcctg gttcgtctca cccgtacaaa tgaacgggtg gcgagcgaca cgtccagtgc
     4441 cgcgggcagt gttgcgcagt gcgcaggtaa tcaacgttcg tgcacgtagg tgccaggggc
     4501 tgtttcgccg gccgggtatt tcttgttgcc cagttgggtc ggtgcctttt tgtgctcacc
     4561 agagcgtgcg gagagccagg tttgccaatg cagccaccag gagtcggcgt gcttggtcga
     4621 gctggcttgc caggtttttg gatccgcgtg catctgggta ttggtcatgt agcgtgcttt
     4681 ggggttaccc ggcgggttga gaatgctttg gatatgcccg ctgttggaca gcacgaactc
     4741 gacattgccg ccgaacaggt tggccgactt gtagcacgac tcccatgggg tgatgtggtc
     4801 ggtggtgccg gccacgcaga agaggtcgct ggtgacctgc ttgaggtcga tcggcgtgcc
     4861 gcagacttcc agcgcacccg cgcggatcag cgggttggag gtaaacatgt cgataaactc
     4921 gccatgcaga gcagctggca ggcgcgtggt gtcgttgttc cagtagagga tatcgaacac
     4981 cggcggctcg ttgccgagca ggtagttgtt gacccagtag ttccagatca ggtcgttggg
     5041 gcgcatccag gcgaacacct tggccatgtc gctgccttcc agcacaccgg cctggtagga
     5101 gcggcgcttg gccatctcta ggcttttttc gtcagcgaac agcgccacat cggtttccag
     5161 acggttgtcc agcacgctga ccagcagggt cagggcatgc acctttttct cacccagcgc
     5221 ggcgtaatgg cccaacagcg aggtggtggt aaggccgccg gaacaggcgc cgagcatgtt
     5281 gatgtctttg ctgccagtga cggcggtgac cacgtcgatg gcttctttca gtgcttcgat
     5341 ataggtcgac aggccccact cgcggtgtgc cttggtcggg ttgcgccagc tcaccacgaa
     5401 ggtttggata ccgttgcgca acaggaagcg tgccacgctc ttttccggcg acaggtcgaa
     5461 gacgtagaac ttgttgatct gaggcggcac caccagcagt gggcgctcgt gcacctgctc
     5521 ggtgatcggc ttgtactgga tcagctcgag caggtcgttg cggaaaatca ccgcgccatc
     5581 ggtggtggcc aagcttttgc cgacctcgaa ggcattcatg ttgacctggc taggcatgcc
     5641 accgttgttg acgatgtcct gagccaggtg cgacagaccg tcgagcaggc tcttgccgcc
     5701 ggtttcgaag aagcgtttga ctgcggccgg gttggccatg ctgttgctgg gggccatggc
     5761 ttcggtcatc aggttgatca cgaagtggcc acggctggca tcctgttcgg acaggttgct
     5821 gtgctagacc cagtcatgca gctccttgcg ccaggccagg taggtctgca ggtagcgacg
     5881 gtacagcggg ttctggctcc aggccggatc ggcaaaacgc cggtcgcctt cgatgggggt
     5941 gaggtcggat tggccgagca tcacgttctt cagctcgatg ctgaagtggg caaggtgttt
     6001 ggcgctgtgc aggggttgct tgagggcttg cttgagcacg attcgggcgg agctaagcag
     6061 gtctttaccg cggatgccga tcaccgggtt aaggcccaaa gtgttctcgg aggcctggtg
     6121 cttcaggtca tcattattct tatctgtcat ctacaacgct ccattgtcct gagacgaaat
     6181 accggcgtaa ggctgtgcac ggcgacacag ggctagaatc ttttccaagt ctgctgcgcg
     6241 agactttgaa caggttccat gaaccaggta ttgcccggat taccaagggc ggatcgctcc
     6301 gcaatattcc tcgtcgcagg ggctgcctgg cgcgagaaaa cttccgacta cttggttacc
     6361 cgagtttgag aatttgtgca agccggccgc gatccaggcc tcatgcgcga tgagtatgca
     6421 gggaaggatt agaaaatgcg ctctaacagg tcaggagcaa cgggctagac gggctggtac
     6481 gccacggcct gttgctgctt gatggcgtgg aggaatacgg aaatagcgga ggtgcctggg
     6541 ccatttccta caggcatgtc gcgcctgcgt ttcaaagcat cagcttgacc acactttcgc
     6601 tagggtcccg cgatttgcct gcggcgctca gggtcgcgag gtaatcgttc cacagctctg
     6661 cctgacgcat ggccagctgc tccaggtaat cccaggtgaa caggccgctg tcgtggccgt
     6721 catcgaaggt cagtttgagg gcgtaattgc cggcgggctc gatcttgttc aggccaacgc
     6781 cgagcttgcc tgtctgcagg atgggcttgc catgtccctg cacctccgcc gacggcgaat
     6841 gcacacgaag gaactcggcg ggcagggtgt agcgctcgtc ggccccgtaa tgcagctcca
     6901 gggttttcga ggctttgtgc agctttatcg cggtaggcat gcgcatggtt gcttcctggg
     6961 ttgctccgta gcccggatag catccgggct acgggcgggc gcgttacagg atatagcgcg
     7021 acagatcttc atcctgcgcc agctcgccga ggtggctgtt gacgtaggca gcgtcaatca
     7081 tgatcggcgc gccattctgc tggccggcca ggtcgccagc gctgaaggaa acctcctcca
     7141 gcaggcgttc gagcagagtg tgcaggcggc gtgcaccgat gttctcggtc ttctcgttga
     7201 cctgataagc aatctcggcg atgcgcttga tgccgtcggc gacaaactgg atgcccaagc
     7261 cttcggtgtt cagcagcgcg gcgtattgct cagtcagcga ggcatgcggc tcggtgagga
     7321 tgcgctcgaa gtcctgcggg gtcagggctt tcagctcgac gcgaatcggc aggcgacctt
     7381 gcagctccgg caccaggtcg ctcggcttgc tcaggtgaaa cgcgccggag gcgataaaca
     7441 ggatgtgatc ggtcttcacc atgcccagct tggtattcac cgtgcagcct tcgatcagcg
     7501 gcagcaggtc gcgctgcacg ccttcgcggg acacgtcggc gccgccggtg ttgccgcgct
     7561 tggcgacctt gtcgatttcg tcgataaaca cgatgccgtt ctgttctacg gcttccaggg
     7621 cctgggcctt aagttcgtct tcattgacca ggcgcgcggc ttcttcgtcg cgcaccatct
     7681 tcaaggcttc cttgaccttg agcttgcggc tcttcttctt gcccttgccc atattggaaa
     7741 ataggctctg cagctggttg gtcatctctt ccatgcccgg cggggtcatg atttcgatgc
     7801 cggccggtgc ttcggccact tcgatatcga tgtccttgtc gtccagctgg ccttcgcgca
     7861 ggcgcttgcg gaacagctgg cgggtgttgg agtcgctgcc cggtgcggct tcttcgctga
     7921 aaccggtgcg ggccggcggc agcagggcat cgaggatgcg ctcttcggcg gcatcttcgg
     7981 cgcggtgacg caccttgatc atctcctgct cgcgcagcat cttgatcgcc gcatcggcga
     8041 ggtcacggat aatcgactcg acatcgcggc cgacatagcc gacctcggtg aacttggtcg
     8101 cttccacctt gataaacggt gcgttggcca gcttggccag gcgacgggcg atttcggtct
     8161 tgccgacgcc ggtcgggccg atcatcagga tgttctttgg cgtcacttcg gcgcgcagtt
     8221 caagcggcag ctgcatacgc cgccagcggt tgcgcagggc gatggccacg gcgcgcttgg
     8281 cgtcgtcctg accgatgata tggcggttga gttcgtgaac gatctcgcgg ggcgtcatgg
     8341 acatgcggtg atactccaga aaaaggcgcg cttattcagc gcagtcttct tcttcgatag
     8401 ttagattctg gttagtgaag acacagatag agccggcaat gttcaatgcg gtttcggtga
     8461 tttcacgggc gctcaggtcg gttttctgca gcagggccat ggccgcggcc tgggcgaagc
     8521 cgccgccgga gcccatggcg atcaggccct gctcgggctc aaccacgtca ccgttaccgg
     8581 tgatgatcag ggaggcgtcc ttgttggcca ctgccagcat ggcctccaga cggcttagcg
     8641 agcggtcggt gcgccagtct ttggccagct cgacggcggc gcgcaccaga tggccctggt
     8701 gtttctccag ctggccttca aagcgttcga acagggtaaa ggcgtcggcg gtggcaccgg
     8761 cgaagccggc cagcacctgg ccgtggtaca ggcggcgaac cttcttcgca ttgcctttca
     8821 tcacggtatt gccgagggac acctggccgt cgccgcccat gacgactttg ccgttgcggc
     8881 gtactgaaac gatggtggtc aaggggagag tctccacgca gcggggcgaa agtgcccaat
     8941 gccgaatgaa gtgggggcag cgggggcccg cttcaagccc tgcagggcac gctgtccgac
     9001 aaatagtcgg cagaacctgt ctcggaatct gctgcgcgtc ggccaaactg cgttgaaaac
     9061 ggcttcggaa tgctcattta cagctcgtaa actccgcttc ctcagccgtt ttcgccttgt
     9121 tttgctctag ctcgcgagat ccgaaacagg ttctgagacg gcactgctgg gcctgaaatc
     9181 gcgcggttta ttgcgcttgg cgctgctgta acaacagatt gctaaagccg ctggcggcca
     9241 gggatttctg cgcctggccg agctgctcgc gggtggcgaa ggggccgacc agtacgcggt
     9301 gccaggtttc ctcgcgtacc gtgccggact ccacctgcac gctttgtccg agcaggatga
     9361 tctgcgcgcg caggctgtcg gcgtcatcct tgcggcggaa cgagccggcc tggaggaaga
     9421 actgcgagct gaccgggccc ttggcgacca ctggcggcgg tggcggcacc tggccgttga
     9481 gcgcggcttc ggcgcgggcg gcatcgatct tcgccgcttc ttccggggta accggcttct
     9541 gcgccggtgg tgcaggcggc tcgatgcctt gcggcggcag gatcacttcc gattccggca
     9601 gcagggtgta gaagtcgtat ttaggcttgg ccggctcgtt gctggctggc ttggcggccg
     9661 ccgctttatt ggcctgagtg acgcgcgcct gttcgacctt ggtgcgcttg atctcgtcgc
     9721 gacccggttc gaggctgaac agaaacacca taaagccgcc gatcaccagg ccgcagacca
     9781 gccagaacca acccggcacc ggctttttcg ctggcgcttg gtaacggctg gcgccgcgtt
     9841 tggcggcggg ctttttcttg gcggccactt acatgcgctc cagggtttcc aggccgagca
     9901 gttccaggcc ctgcttgagc gtgcgcgcgg tcagtgcggc caggcgcagg cggctgttgc
     9961 gcagcgcctc gtcctcggtg gagaggatcg ggcagttctc gtagaagctg gagaacagtc
    10021 cggccaggtc gtacaggtaa gcgcagagca ggtgcggctc gcccttgtcg gcgacgctgg
    10081 caagcacttc ggcgaactgc gcgagtttgc cggccagttc gatttcgtga gcggcctgca
    10141 ggtcgaggtt gccgccgatc tcgtcgaaac ccttgcccag cttgcggaat acgctggcca
    10201 cgcgggtgta ggcgtacagc agatacggcg cggtgttgcc ctcgaagctc agcatcagct
    10261 cgaagttgaa gcggtagtcg ctggtgcggt gcttggacag gtcggcgtat ttcaccgcac
    10321 cgatgcccac cgcgcgagcg atctggcgca gctcggcttc gtccagttca gggttcttgc
    10381 ctttgaccag ggcgtaggcg cgctcttcgg cttcatcgag caggtcgacc agcttcaccg
    10441 tgccgccatc gcgggtcttg aacgggcggc catcggcgcc gttcatggtg ccgaagccca
    10501 tatgttccag ctgcatgttg gccgggacga agccagccag gcgcgcggca gtgaagacca
    10561 tttggaagtg cagggcttgg cgttgatcga cgaagtacag cgcgcgatca gctttcaata
    10621 cattggcgcg gtagcgggta gctgccaggt cggtggtggc gtacaggtag ccgccgcctg
    10681 ccttctgcac gatcagcggc agcggattgc cttcggcgtt cttgaactcg tccataaaca
    10741 cgcactgggc gccgtcgctt tcgctgagca ggcccttggc cttgaggtcg gcgaccacgt
    10801 tggccaggtc gtcgttgtag gcgctttcac ctttcacgtc ggccatgctc agcttcacgc
    10861 caaggcggtc atacagcgcc tggcagtggc tcagggaaat atcgttgaaa cggtgccaca
    10921 ggcgcaggca ttcggcgtcg ccggcttgca gctgcaccac cagctcgcgg gcacgctcgg
    10981 cgaactcagg ggattcgtcg aagcgcttct tcgccgcgcg gtagaaacct tccaggtcag
    11041 ccagctcgct ttcggcggcg gccgggtttt cctgcataaa ggccagcagc atgccgaact
    11101 gggtgcccca gtcgcccacg tggttctgcc ggatcacttc gtcaccgagg aattccagca
    11161 cccgcgccac accgtcaccg atgatggtcg agcgcagatg gccgacgtgc atttccttgg
    11221 ccaggttggg ggcggacagg tcgaccacca cgcgctgcgc cggaccagcc ttgcgtacgc
    11281 cgagcttggc gtcggccagc agggcatcca ggcgcgcggc cagggcctgg gtgttctggt
    11341 agaaattgag gaagcccggg ccggcgatct cggccttgct gatcgcagca tcgctaggca
    11401 gcgcggcgat cagcttctcg gccaggtcac gcggcttcag gccggccggc ttggccagca
    11461 tcatggcgat attgctggcg aagtcgccgt gggtcttgtc cttggtgttc tccacctgaa
    11521 tggccgggct caggccttcc ggcagcacgc cgtcggcggt gaggcgggtc agggcttgct
    11581 ggatcaggtg gcgaatgctg tctttcatgg tctgctcggt cggccggtgg tgacggcggc
    11641 gcacaaaggc gctggttgaa aactgcgcat tatcgacgcc cttcgcccgc agcttcaagc
    11701 agcgtgtggt ttcgccgggc taaaggccgc cgctatgggc tgcagatcac gccgcgccgg
    11761 gcctctataa ggaagcgccg cagaggcgaa accgctgcgg cgtctggatg tagcgcgcag
    11821 cgttacgcgg aacgctgcgc cgtgccacgc gcggcggcgt tgccggcgac gaaatacggc
    11881 gcggtgctgc gtggcagcgg ggcgcggccc cggattttgt cggcgatctt ctcggccatc
    11941 atgatggtcg gtgcattcag attgccggtg acgatcagcg gcatgatcga cgcatccacc
    12001 acgcgcaggc cctgcacacc gtgcacccgg ccctggccgt cgaccacggc catgtcgtct
    12061 tcgcccattt tgcaggagca cgacggatgg taggccgttt ctgcatgttc gcggataaaa
    12121 gcgtccagtt cggcgtcgct ttgcagctcg gcaccggggc tgatctcgcg gccgcgatac
    12181 gggtcaaggg cggattgcgc catgatttcg cgggtgatgc ggatgccgtc gcggaactcg
    12241 cgccagtcct gctcgtgcgc catgtagtta aagaggatgc tcgggtgctg gcgcgggtcc
    12301 ttggatgtga cctggatgcg gccacggctc ggcgagcgca tggagcccat atgcgcctgg
    12361 aagccgtgct ccttcaccgc gttgctgccg ttgtagttaa tcgccaccgg cagaaagtgg
    12421 tactggatat tcggccattc gaattcagcg ctggagcgga taaagccgcc ggcctcgaac
    12481 tggttgctgg cgccgatacc tttgccgagg aacagccagt tggcgccgat catcggctgg
    12541 ttccaccatt gcagcgccgg gtacagcgac accggctgct tgcaggcgta ttgcaggtac
    12601 acctccaggt ggtcctgcag attctggccg acgccgggca ggtcgtgcac caaggggatg
    12661 tccagctcgc gcagcagcgc cgccgggccg acgccggagc gttgcagcac ctgcggcgag
    12721 gcaatcgcgc cgccgcacag cagcacttcg cggcgcgcgt tgacggtgat cggtgtgtcg
    12781 ttatcgccca gcagatacgc cacgccgctg gcgcgcttgc cgctgaacag gatgcggtca
    12841 gtcagggcat gggtgacgat ggtcagattc ggccgttcgc gggcctgatc aaggtaaccg
    12901 cgcgcggtgc tggcccggcg gcccttgggc gtaacggtgc ggtccatcgg gccgaagcct
    12961 tcctgctggt aaccgttgag gtcgtcggta cgcgggtagc cggcctgcac gccagcttcg
    13021 atcatggcgt ggaatagcgg gttattgccg ttcttcggcg tggttaccga cacggggcca
    13081 tcgccgccat ggtaggcatt ggcgccgata tcacgggttt ccgctttgcg aaaatatggc
    13141 aggcaatcca gatagctcca gtcttccagg cctttttcct cagcccagcc gtcgtaatcc
    13201 atggcgttgc cgcggatgta gcacatgccg ttgatcaggg acgagccgcc caatcccttg
    13261 ccgcgcccgc attccatgcg gcggttgttc atatgcggct cgggttcggt caggtaagcc
    13321 cagttgtagc ggcggccctg cagcgggaag gcgagggcgg cgggcatctg cgtgcgaaaa
    13381 tccaggcggt aatccgggcc gccggcttcc agcagtagca cgctgacgtc agcgtcttcg
    13441 gtcagacggg tggccagcac gttaccggcc gagccggcgc cgatgatgat gtagtcgaat
    13501 tcctggggca taaaagctcc tgtagcccgg atgcactccg gggaaaacgc ggtgactgtt
    13561 cccggattgc atc
//
LOCUS       DASRUM010000061        12195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1039629, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000061 DASRUM010000000
VERSION     DASRUM010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 12195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12195
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039629"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..2173)
                     /gene="fadB"
                     /locus_tag="VFF22_04435"
     CDS             complement(26..2173)
                     /gene="fadB"
                     /locus_tag="VFF22_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016780046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HZX16102.1"
                     /translation="MIYEGKAITVKALESGIVELKFDLKGESVNKFNRLTLNELRQSV
                     DAIKADGSIKGVIVTSGKDVFIVGADITEFVDNFKLPDEELVAGNLEANKIFSDFEDL
                     NVPTVVAINGIALGGGFEMCLAADYRVMAESAKVGLPEVKLGIYPGFGGTVRLPRVIG
                     TDNAVEWIAGGKENKAADALKMGAVDAVVAADKLQAAALDLIKRAISGELDYKAKRQP
                     KLEKLKLNAIEQMMCFETAKGFVAGQAGPNYPAPVEAIKTIQKAANFGRDKALEVEAA
                     GFVKLAKTPVAASLIGLFLNDQELKKKSKQYDEIAKDVKLAAVLGAGIMGGGIAYQSA
                     SKGTPILMKDIREEGIQMGLAEASKLLGGRVAKGRMTAAKMAEALTAIRPTMSYGDFG
                     AVDIVVEAVVENPKVKQIVLAEVEGVVKEDAILASNTSTISINLLAKALKRPENFVGM
                     HFFNPVHMMPLVEVIRGEKSSDVAVATTVAYAKKMGKTPVVVNDCPGFLVNRVLFPYF
                     GGFSKLLGFGVDFVRIDKVMEKFGWPMGPAYLSDVVGIDTGHHGRDVMAEGFPDRMAV
                     EGRTAVDVMYEADRLGQKNGKGFYSYEMDKRGKPKKVSDPVAYDLLKSIVTEQREVTD
                     EDIINFMMIPLCMETVRCLEDGIVDTAAEADMGLIYGIGFPPFRGGALRYIDTIGVAE
                     FVALADKYAELGALYTPTAKLREMAANGQKFFG"
     gene            2654..3472
                     /locus_tag="VFF22_04440"
     CDS             2654..3472
                     /locus_tag="VFF22_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16103.1"
                     /translation="MSSRMLAIVLSTLLAITLPLFSAMTQAADAATSLLKLHQLRLAA
                     QKSLGDFYMFNGMEGDQRYARMINDSVSEVNNHLTALTDMPGEGAKALRSQLDQQWKG
                     YEIDLNNLINALKSQGYTDLQPVADLAARNQQLMTLSQELYTKIQQEGSYSVPALTQR
                     SREQSMLMQGIAVDYASRSASVGATFMGGGDARPIEELVSEFASKMAALEKDPKNTPQ
                     LKQSWEGVATKWRYIEKSLINYNENSVPFLVNKYSNTIIQGIEQISSQYAAANL"
     gene            complement(3556..3993)
                     /locus_tag="VFF22_04445"
     CDS             complement(3556..3993)
                     /locus_tag="VFF22_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16104.1"
                     /translation="MSPWFLLLLLAFLLSPLAWLVPSRHQRGQMDVRLQARRMGVAMQ
                     LSRQEWPHWLLRQPPHSCPQYHRARRRGQSDSWCYWQLEPGHWVNKWREPCADQALLT
                     QLQALPADAFKVEATDQMLSVCWGERGAREALEAIVAFLQAKA"
     gene            4081..4521
                     /locus_tag="VFF22_04450"
     CDS             4081..4521
                     /locus_tag="VFF22_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZX16105.1"
                     /translation="MPYQHILIAVDLTEECDPVMHRAQKLAASSGAKLSVVHIVEPMA
                     MAFGGDVPMDLSMLQQQQFDQARERLDKFAGKYPDLSAEHRHLAYGQPRQEIHRLAEE
                     QQCDLIVVGSHGRHGLALLLGSTANDVLHGAPCDVLAVRLKKVE"
     gene            complement(4552..5238)
                     /locus_tag="VFF22_04455"
     CDS             complement(4552..5238)
                     /locus_tag="VFF22_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16106.1"
                     /translation="MAIEYRITLDDNHQFSYRIELDRVYEAEVAQSAPAWTRLEYQQC
                     SNCPLSKDNFSHCPAAVDLHRVIEDFQGLPAFKKVAVWVRTPEREYNKQVGLEEGLRA
                     LLGVIMATSACPVLGKLKPMAQHHLPFANSQEFILRTVSLYLTQQYFNLREGRTADWE
                     LKGLVRMFQQLQLVNQAFWQRIHATCEGDSNLKAFLTFFSMASSMTYSLETQLQKIRP
                     LVMSSPDSGE"
     gene            complement(5302..7215)
                     /locus_tag="VFF22_04460"
     CDS             complement(5302..7215)
                     /locus_tag="VFF22_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZX16107.1"
                     /translation="MTLLKFTDISLAYGAMPLLDKVSWQIARGERVCIIGRNGTGKSS
                     MMKLVKGDQKADDGAVWRAPGLKIGELPQELPQADDRTVFDVVAQGLDGVGELLAQYH
                     HLAQNCVTEADLDKLMHVQQDLEARDGWRLQQLVDSTLSRLQLPADKTLAELSGGWRR
                     RVLLAQALVSEPDLLLLDEPTNHLDIGAIAWLEEALTDFQGAVLFITHDRSFLQNLAT
                     RILELDRGGLIDWNGDYASFLVHKEATLAAEETANALFDKRLAQEEVWIRQGIKARRT
                     RNEGRVRALKALRVERSERRERTGKANIMLETAEKSGKQVMVLENVSFAHPGGPFLIK
                     DFSMVLQRGDRIGLLGANGTGKTTLLKLMLGGLQPTSGEVAEGTRIDVAYFDQLRHQL
                     DLEKTVIDNVAEGRDFIEIDGQNRHVLSYLGDFLFSPQRARTPVKALSGGERARLLLA
                     KLFSKPANLLVLDEPTNDLDVETLELLEEVLLTFPGTVLMVSHDRAFLDNVVTSTLVF
                     EGEGRVREYVGGYQDWLRQGGSPRLLGVGESKAGKPELASAVVPAPVAVASVVVESSK
                     KKLSYKLQRELEAIPGQIDALEGQVASLQEQIGSSSFYQQPAEKTAEVLAKLQAVQHE
                     LEQLVERWAELEG"
     gene            7597..9522
                     /locus_tag="VFF22_04465"
     CDS             7597..9522
                     /locus_tag="VFF22_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZX16108.1"
                     /translation="MRGPLFSLLYCLLVSSLSVTAAQAATLTQQRQYYDQAKAALAKG
                     DKGPYQRYASALRDYPLEPYLAYDELTARLKWASNDEIEKFLAEHGDLPQISWMKLRW
                     LRWLAERGEWQTFINYYDPALNFTELDCLYGQYQLSHGMRAEGNATAEKLWLVGKSQP
                     NACDPLFAMWSAEGQLTEQRRWQRAKLAAEARNYGLVTHLIKTMPTLGNHGKTLLQVA
                     QKPELLKQTSRFTQTDPAMADVVGLGLRRLARQDPESALKLLDGYAQRMKFSSEEQVA
                     IAREIGLTLAKRFDSRALQVMAKYDPELRDNTVSEWRARLLLRLGRWDEANQLTRRMP
                     AELGNTNRWRYWQARSLQLAQPNSQEPKALYQALAKERDFYGFMAADQVQAPYQLNNK
                     PLALSPQTIQKVRNSAAIRRALEFHARGQVADGRREWYNVSRLFSRDEMVAQARLAYE
                     MGWYFPAIRTISQAQYWDDLDVRFPMAHREHLVREAKNRDIHSSWVFAVTRQESAFMA
                     DAKSGVGAMGLMQLMPATAKETAKRFGIPLSSPQLAYRPEINIQLGAAYLSQIYGQFN
                     GNRVLASAAYNAGPGRVRQWLRGADHLSYDVWIENIPFDETRQYVQNVLSYSVIYGEK
                     LNAPQPLVAWHERYFDQ"
     gene            complement(9572..10378)
                     /locus_tag="VFF22_04470"
     CDS             complement(9572..10378)
                     /locus_tag="VFF22_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016852662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HZX16109.1"
                     /translation="MLHLSGLYRYPLKSAAGEALRETALDALGVQGDRRWMVVDAETG
                     RFLTQRLLTQMTQLQARWLGGTHLQLSAPGMAELQVTVPDEQAPLRGVTIWRDSLQVP
                     DAGEAAAQWLSQFLGRACRLVQVSEPRARQVDTAYAEAGDKVAFADGFPLLLIGQASL
                     DDLSARVGRPLPMLRFRPNLVVSGSEPYAEDSWKRIRIGELEFRVVKGCSRCIMTTLD
                     PETGERSADREPLTTLKTYREREGEVYFGQNLIACGEGQLRLDMPVQVLE"
     gene            10377..10856
                     /locus_tag="VFF22_04475"
     CDS             10377..10856
                     /locus_tag="VFF22_04475"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HZX16110.1"
                     /translation="MNPASLWQNALIRATRRTHKPVLTLPLLVLLSACSGTAPDNLGI
                     REGRLASCPDSPNCVNSQASDARHAIEPLPLQGSAEQTQARLKALLSEEPRVNLVEEA
                     PGYLRAEFSSKLMRFVDDVEFLIGAAAVDVRSASRLGYKDFDVNRERIEHLRQRLGE"
     gene            complement(10874..11809)
                     /locus_tag="VFF22_04480"
     CDS             complement(10874..11809)
                     /locus_tag="VFF22_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheV"
                     /protein_id="HZX16111.1"
                     /translation="MAGILDSVNQRTQLVGANRLEILMFRLAGRQLFAINVFKVQEVL
                     QMPKLTLMPQRHPFVCGVVSLRGQTLPVIDLSQAIGMRAQVPSETSTIIVTEYNRSVQ
                     AFLVGGVDRILNLNWESILPPPGGAGRQHYLTAITKVDDQIVEVIDVEKGLSEISPMS
                     SKVSAEKLSDPLLEKAKGREVLLVDDSNVALTQLKETMAQLQIRTHVASDGLKALKML
                     QLWADSGVDMQEKLLMVITDAEMPEMDGYRLTTEIRSDPRLKDLYVVLHTSLSGSFNE
                     AMVKKVGCDNFLSKFQPDKLVDVVHERLALIHPQE"
     gene            complement(11964..>12195)
                     /locus_tag="VFF22_04485"
     CDS             complement(11964..>12195)
                     /locus_tag="VFF22_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HZX16112.1"
                     /translation="APADVVGTLGTLLSGGINGLQSVALNARLPWLEIEAPEGLDMNL
                     DGEPMESSRLRFAAMPAALRVHLPADSPLLRD"
BASE COUNT         2616 a         3844 c         3388 g         2347 t
ORIGIN      
        1 cactctcgct ctagtctgtc cgcgattaac cgaaaaactt ctggccgtta gcggccattt
       61 cgcgaagctt ggcagtcggg gtgtacagcg cgcccagctc ggcgtatttg tcggccaggg
      121 caacgaactc ggctacaccg atggtatcga tataacgcag cgcaccaccg cggaacggcg
      181 ggaagccaat gccgtagatc aggcccatat cggcctcagc tgcggtatca acgatgccgt
      241 cttccagaca acgcacggtt tccatgcaca gaggaatcat catgaagttg atgatgtctt
      301 catcagtcac ttcacgttgc tcagtcacga tggacttcag cagatcgtag gcaaccggat
      361 cagaaacctt cttcggcttg ccgcgcttgt ccatttcgta ggagtagaag cctttgccat
      421 tcttctggcc caggcgatct gcttcataca tcacgtcaac tgcggtacgg ccttcaacag
      481 ccatgcgatc cgggaagcct tcagccatta cgtcacggcc gtggtgacca gtgtcgatac
      541 cgaccacgtc ggacaggtac gccgggccca ttggccagcc gaacttttcc atgactttgt
      601 cgatacgcac gaagtccaca ccgaaaccga gcagcttgga gaagccgccg aagtacggga
      661 acagcacgcg gttaaccagg aagcctgggc agtcgttgac cacaaccggg gtcttgccca
      721 ttttcttggc gtaagcaacg gtggtagcca cggctacgtc gctggacttc tcgccacgaa
      781 tcacttcaac cagcggcatc atgtgcacag ggttgaagaa gtgcatgccg acgaagtttt
      841 ccggacgctt gagggccttg gccagcaagt tgatggaaat tgtcgaggtg ttcgacgcaa
      901 ggattgcgtc ttctttaacc acgccttcca cttcagccag cacgatctgc ttgaccttcg
      961 ggttctcgac cacggcttcg acaacgatgt cgacagcgcc gaagtcgccg taggacatgg
     1021 tcggacgaat agcagtaagg gcttcagcca tcttcgccgc agtcatacga cctttggcaa
     1081 cgcgaccgcc gagcagcttg gaagcctcgg ccaaacccat ctggataccc tcttcacgga
     1141 tatccttcat caggattgga gtgcccttgg aagcggactg gtaggcgata ccgccaccca
     1201 tgatgcccgc gccgagtacg gcagccagct tcacgtcttt agcgatttcg tcgtactgct
     1261 tggacttctt cttcagctcc tgatcgttca ggaacaggcc gatcaggctg gcagcaaccg
     1321 gggttttagc cagtttgacg aaaccagcag cttccacttc cagcgctttg tcgcgaccga
     1381 agttggcggc tttctgaatg gtcttgatcg cttcaaccgg cgcagggtag ttcgggccgg
     1441 cctggcctgc aacaaagcct ttggcggttt cgaagcacat catctgctcg atagcgttga
     1501 gcttgagctt ttccagcttc ggctgacgct tggccttgta gtccagctcg ccggagatgg
     1561 cgcgcttgat caggtccagg gcagcggctt gcagcttgtc agcggcaacc acggcatcca
     1621 ctgcacccat tttcagagcg tcagcagctt tgttttcttt accaccggca atccactcaa
     1681 cggcgttatc ggtaccgata acccgtggca gacgcacggt gccgccgaag cctgggtaga
     1741 tgcccagttt gacttccggc aggcccactt tggcgctttc ggccatgacg cggtaatccg
     1801 cggccaggca catctcaaaa ccaccgccca gggcgatgcc gttgatcgcg acaacagtcg
     1861 gaacattgag gtcttcaaaa tcactgaaga tcttgttggc ttcgaggttg ccagccacca
     1921 gttcttcgtc cggcaacttg aagttgtcga cgaactcggt gatgtcggcg ccgacgataa
     1981 atacgtcttt gccactggtg acaatcacgc ccttgatcga accatcggcc ttgatcgcat
     2041 caacggattg acgcagttca ttgagggtaa gacggttgaa tttgttgacg gattcaccct
     2101 tgaggtcgaa tttcaattcg acaatgccac tctcaagagc cttaaccgtg atggctttac
     2161 cttcgtaaat catcaactga tctccacggt atggaagctg aacagtacac atcggagcca
     2221 accagcaagc tactccacga catagccgtc tcagagtagc cccaatcagc ctggcacacc
     2281 caccgacgcg ataatcgggc tgtaaaggcg gcgcctttga aggcaaacgc tcaattcata
     2341 cgcccgtttg atttgggtgt gcacaccttc aaggaaaagc ccttacttgt caattggcct
     2401 gtggaggcgg caaaggcgta caaaaaatta cgttccggct tacaccaacg tacctccccc
     2461 ctgcagcgaa caggcggcca tctgctgcga tatacgatta cttggcaatc tctgaaaaca
     2521 gaagtgtgat ccagcgcata cctagcaaaa tgatttagct gcaggtacag tcgcctcaat
     2581 acggccgttt tagacagccc gtagccaaaa caaaaaaatt agagcccaat caaagggcac
     2641 acggagtttt cccatgtcga gccgcatgct tgccattgtt ttgagcaccc tgctcgcgat
     2701 caccctcccc cttttcagcg ccatgaccca ggctgcagat gccgccacca gcttactgaa
     2761 gctgcaccag ctacgcctcg ctgcacaaaa atccctgggc gacttctata tgttcaacgg
     2821 catggaaggc gatcaacgct atgcgcggat gattaacgac tcagtgagcg aggtgaataa
     2881 ccacctgacg gcgctaacgg atatgcccgg cgaaggcgcg aaagccctgc gctcgcagct
     2941 ggaccaacaa tggaagggtt acgagattga cctcaacaac ctgatcaacg cattgaagag
     3001 ccagggttac accgacctgc agccggtagc cgacctggca gcacgcaacc agcaactgat
     3061 gaccctgagc caggagctgt ataccaagat ccagcaggaa ggcagctaca gcgtgcctgc
     3121 cctgacccag cgcagccgtg aacagagcat gctgatgcaa ggcattgctg tggattacgc
     3181 ctcgcgcagc gcctcagtag gcgccacctt tatgggcggc ggcgacgcac ggccaatcga
     3241 agagctggtc agtgaattcg ccagcaagat ggcggcgctg gagaaagacc cgaagaacac
     3301 gccgcagctc aagcaatcat gggaaggggt cgccaccaaa tggcgctata ttgaaaagtc
     3361 gctgatcaac tacaacgaaa acagcgtgcc atttctggtc aacaaatact ccaacaccat
     3421 catccagggc attgaacaaa tttccagtca gtacgcggcc gccaacctct gatcgcgacc
     3481 gccgccatcc gcattcgttt taccggcctg cctggccgta ctccggccca gtgctcgcac
     3541 tgggccttct tttattcagg ctttggcctg gagaaacgcg acaattgcct ccaacgcttc
     3601 gcgagccccc cgctcacccc aacataccga aagcatttga tcggtcgcct cgaccttgaa
     3661 tgcatccgcc ggcagcgcct gcaattgcgt caacaaggcc tgatcagcgc aaggctcgcg
     3721 ccatttattc acccagtgcc ccggttccag ctgccaatag caccagctat cagactggcc
     3781 gcgacggcgt gcacggtgat attgcgggca ggagtgcggc ggctgacgca gcagccaatg
     3841 cggccattct tggcgcgaca actgcatcgc cacgcccata cgccgcgcct gcagacgcac
     3901 atccatctgc ccgcgctggt ggcgcgaggg caccagccag gccagtgggc tgagcaaaaa
     3961 ggccaggagc aaaagcagaa accaaggact catatcgtta tctcaccagc aaaagtctgt
     4021 catcatcgct caggacggcc atactaaaac cattgaacca cttgcaagga gagttacccc
     4081 atgccctacc agcacattct gatcgccgtc gaccttaccg aggaatgcga cccggtcatg
     4141 caccgcgcgc agaaactggc cgccagcagc ggggccaagt tgtctgtggt gcatatcgtc
     4201 gaacccatgg ccatggcctt tggcggcgac gtaccgatgg acctgtcgat gctgcaacag
     4261 cagcagttcg accaggcccg cgaacgcctg gacaagtttg ccggcaagta tcccgatctc
     4321 agcgccgaac atcgccacct ggcttacggt caaccacgcc aggaaattca tcgactggcc
     4381 gaagaacaac agtgcgatct gatcgtcgtc ggcagccacg gccgccatgg cctggcgctg
     4441 ctattgggct cgacggccaa cgacgtactg cacggcgcgc cctgcgatgt gctggccgtg
     4501 cgcctgaaaa aagtcgagta acgcgagctg cgagtgaata acgcggctgc gttattcacc
     4561 gctatcgggt gagctcatga ccagtgggcg aatcttctgc agctgggttt ccagcgaata
     4621 ggtcatgctc gacgccatgg agaagaaggt caggaacgcc ttgaggttgg aatcaccctc
     4681 acaggtcgcg tgaatccgct gccagaacgc ctgattgacc agctgcaact gctgaaacat
     4741 acgcaccagg cctttcaact cccaatccgc cgtgcgcccc tcgcgcaaat tgaagtactg
     4801 ctgagtcagg tacaacgaca ccgtacgcag aataaattcc tggctattgg caaacggcag
     4861 atggtgctgc gccatgggtt tgagcttacc cagcaccggg caagcgctgg tggccatgat
     4921 aaccccgagc agtgcgcgca ggccctcttc aagacccacc tgcttgttgt actcgcgctc
     4981 aggcgtacgc acccacacag ccaccttctt gaacgccggt aggccctgga aatcctcaat
     5041 gacccggtgc agatcaacag ccgccggaca atgactgaag ttgtccttgc tcagtgggca
     5101 gttgctgcac tgctgatatt caagacgcgt ccaggccggt gcgctttgcg caacctctgc
     5161 ctcatacacc cggtccagct cgatcctgta gctgaactga tgattgtcgt cgagagtgat
     5221 gcggtactcg atggccatga ctgtctccgg cagaggtctc aaaatcctgt ttgagcgcgt
     5281 tacgtactta atacatcggg tttagccttc cagctcggcc caacgctcga ccaattgctc
     5341 cagctcatgc tgcacggcct gcagtttcgc cagcacctca gcggtctttt ccgcaggctg
     5401 ctgatagaaa ctcgacgagc cgatctgctc ctgcaaactg gcaacctgcc cttccagtgc
     5461 atcgatttgc cctgggatgg cttccagctc gcgctgtagc ttgtaactga gctttttctt
     5521 gctgctctcc accaccacag acgccacggc aaccggagcc gggaccactg ctgaggctag
     5581 ttcaggcttg ccggctttgc tttcgccaac cccgagcagg cgcggcgaac cgccctgacg
     5641 cagccaatcc tgataaccgc cgacgtattc gcgtacgcgc ccttcacctt cgaagaccag
     5701 ggtgctggtc accacgttgt cgaggaaggc ccggtcgtgg ctaaccatca gcacggtgcc
     5761 cgggaaggtc agcagcactt cttcaagcag ctcgagggtt tccacatcga ggtcgttggt
     5821 cggttcatcc agcaccagca ggttggccgg cttgctaaac agcttggcga gcaagagacg
     5881 tgcccgctca ccgccggaca gcgccttgac cggggtacgt gcacgctgtg ggctgaacag
     5941 gaagtcgccc aggtagctca gcacatggcg gttctgcccg tcgatctcga taaagtcgcg
     6001 gccttcggcg acgttgtcga tcacggtctt ttccagatcg agctggtgac gcaattggtc
     6061 aaaataagcc acatcgatgc gtgtgccttc ggccacttca ccgctggtgg gctgcaaacc
     6121 gccgagcatc agcttgagca aggtggtttt gccagtgccg ttggcgccga gcagaccgat
     6181 gcggtcgccg cgctgcagca ccatggagaa gtccttgatc aggaacggcc cacctggatg
     6241 cgcaaaactg acgttttcca gcaccatcac ctgcttgccg gatttctccg cagtttccag
     6301 catgatattg gccttgccgg tgcgctcacg gcgctcgctg cgctccacac gcagggcttt
     6361 cagggcgcgt acacggcctt cattacgtgt gcggcgggcc ttgatgccct ggcgaatcca
     6421 cacttcttcc tgagccaggc gcttgtcgaa cagcgcgttg gcggtttctt cggcggccag
     6481 ggtggcctcc ttgtgcacta ggaagctggc gtagtcgccg ttccagtcga tcaggccgcc
     6541 gcgatccagt tcgaggatgc gcgtagccag gttctgcagg aaggaacggt cgtgagtgat
     6601 aaacagcaca gctccttgga aatcggtcaa ggcttcttcc agccaggcaa ttgcgccgat
     6661 atccaggtgg ttggttggct cgtcgagcag cagcagatcc ggctcggaca ccaacgcctg
     6721 ggccagcagc acgcgacgac gccagccacc ggagagctca gccagggtct tgtcggccgg
     6781 cagttgcagg cggctcaggg tactgtcgac cagttgctgc aagcgccagc catcacgggc
     6841 ttcgaggtcc tgctggacgt gcatcagctt gtccagatcg gcctcggtga cacagttctg
     6901 cgccaagtgg tggtattgcg cgagcagttc acccacaccg tccaggcctt gggcgaccac
     6961 atcaaacacg gtgcggtcgt cggcttgcgg caattcctga ggcaattcgc cgatcttcag
     7021 gccaggtgcg cgccagacgg cgccgtcatc ggccttctgg tcgcccttga ccagcttcat
     7081 catgctcgac ttaccggtgc cgttgcggcc gatgatgcac acccgctcgc cacgggcgat
     7141 ctgccaagac accttgtcca atagcggcat tgcgccgtag gccagagaaa tatcggtgaa
     7201 cttgagcagg gtcatggcac gcctcaacaa aaaacgacgg gtgaacgctg gtaaaaccgc
     7261 ggccgagcaa cgggcttcgt tgcattcaag gcacagcggc aacctgaaac acccagaaaa
     7321 tcatctgcag ctgcccaagg cacccgcaaa acagggcgcg cattctaacc gagaagcgta
     7381 ctggccgagc aggcaatttc ggccgcaggc gcagtaaatt cagcgctgac catgcgatat
     7441 ccaggcggca aaggctctag cgcgcatgca aaggcttaag cagagcaacc ggcaacgatt
     7501 actgccgaca attacgcgtt gaaacgcttt gcgccttcac cgcccatagg caaacgacta
     7561 agctagggcc atacgcacta tttgcctgga agtgtcatgc gcggtcccct gttcagcctg
     7621 ctgtactgcc tgttggtttc ctccctgagc gtcactgcgg cgcaggccgc caccctgacg
     7681 caacaacgtc aatattacga tcaagccaaa gccgcgctgg ccaagggcga taaaggccct
     7741 taccaacgct acgccagtgc cctgcgcgac tacccgctgg agccttacct ggcgtatgac
     7801 gagctgaccg cccggctgaa atgggcgagc aatgacgaaa tcgaaaaatt cctcgccgaa
     7861 catggcgacc ttccgcagat cagctggatg aaactgcgct ggctgcgctg gctggccgag
     7921 cgcggcgaat ggcagacctt tatcaactac tacgacccgg cgctgaactt caccgagctc
     7981 gactgcctgt atggccaata ccagctgagc cacggcatgc gcgccgaagg caacgccact
     8041 gcagaaaaac tctggctagt gggtaaatcg cagcccaacg cctgtgaccc gctgttcgca
     8101 atgtggtccg ccgaaggcca actcaccgag caaagacgct ggcagcgcgc caagctggcg
     8161 gccgaagcgc gcaactatgg gctggtcact cacctgatca agaccatgcc aaccctgggt
     8221 aaccatggca aaaccctgct gcaggttgcg cagaagcctg aactgctgaa gcagacctcg
     8281 cgctttaccc aaaccgaccc ggccatggcc gatgtagttg gcctcggcct gcgccgcttg
     8341 gcccgccagg acccggaaag cgcgctcaag ctgctcgatg gctacgccca acggatgaaa
     8401 ttctccagcg aggaacaggt cgccatcgcc cgggaaatcg gcctgaccct ggccaagcgc
     8461 ttcgactccc gcgctctgca ggtcatggcc aagtacgacc ctgaactgcg tgacaacacc
     8521 gtcagcgaat ggcgcgcccg cctgctgctg cgcctgggcc gttgggatga agccaaccag
     8581 ctgacgcgcc ggatgcccgc ggaactcggc aacaccaacc gctggcgcta ctggcaggcg
     8641 cgtagcctgc aactggcgca gcccaatagc caggaaccaa aagcgctgta ccaggcgctg
     8701 gccaaggagc gtgacttcta cggctttatg gccgccgacc aggtgcaggc cccctatcag
     8761 ctgaataaca agccattggc cctcagcccg cagaccatcc agaaagtgcg taactcggca
     8821 gcgattcgcc gcgccctgga attccacgcc cgtggccagg tcgccgatgg ccgccgtgag
     8881 tggtacaacg tcagccgcct gttcagccgc gatgaaatgg tcgcccaggc gcgcctagcc
     8941 tatgagatgg gctggtactt cccggccatc cgcaccatca gccaggcgca gtattgggac
     9001 gacttggacg tccgcttccc catggcacac cgcgaacatc tggtgcgcga agcgaagaac
     9061 cgtgacatcc actccagctg ggtatttgcc gtcacccgcc aggaaagcgc ctttatggcc
     9121 gatgccaagt ccggcgtggg ggccatgggc ctaatgcagc tgatgccggc caccgccaag
     9181 gaaaccgcca aacgctttgg tattccgctg tcatcgccac aactggccta ccgcccggaa
     9241 atcaatatcc agctgggtgc ggcctacctg agccagatct acggccagtt caacggtaac
     9301 cgcgtgctcg cctctgccgc ctacaacgcc ggtccaggcc gggtacgcca gtggctacgc
     9361 ggcgcggatc atctgtcgta tgacgtgtgg atcgagaaca tcccgtttga tgaaacccgc
     9421 cagtacgtgc agaacgtgct gtcctactcg gtgatctacg gcgagaagct caacgcgccg
     9481 cagccactgg tggcctggca cgagcgttac ttcgaccagt aacggctaaa taggcacgct
     9541 caactgcaac ggttgagcgt gcattgctcg cttattccaa cacctgcacc ggcatgtcca
     9601 gacgcaactg cccttcgcca caggcaatca ggttctgtcc gaaatacacc tcgccttcac
     9661 gctcacggta ggttttcagg gtggtcagcg gttcgcgatc ggcactgcgc tcgcccgtct
     9721 ctgggtcgag ggtggtcatg atgcagcgcg agcagccctt gaccacacga aactccagct
     9781 caccgatacg gatgcgcttc cagctgtctt cggcataagg ctcactgcca ctgacgacca
     9841 ggttcgggcg aaaacgcagc atcggcaacg gccggccgac ccgcgccgac aggtcatcca
     9901 gcgacgcctg gccgatcagc agcaaaggga agccatcggc aaacgccacc ttgtcgcccg
     9961 cctcggcata ggccgtatcg acttgccgcg cacgcggctc cgacacctgt accaggcggc
    10021 aggcacgacc gagaaactgg ctcaaccact gcgcggccgc ttcgccagca tccggcacct
    10081 gcaagctgtc gcgccagatg gtcacgccgc gcagtggcgc ctgctcatcc ggcacagtca
    10141 cctgcagttc agccatgccc ggcgcactca gttgcaggtg cgtgccgccc agccagcgcg
    10201 cctgcagctg ggtcatttgc gtcagcaaac gttgcgtaag gaagcgcccg gtttcagcat
    10261 cgactaccat ccagcgacga tcaccctgca cgcccaaggc gtccagcgcc gtttcgcgca
    10321 aggcctcgcc tgcagccgac ttcagtggat aacggtacaa cccggagaga tgcaacatga
    10381 acccagcttc cctttggcaa aacgcactta tacgagcaac ccgccgcact cacaagcccg
    10441 ttttaacgct gccgttgctg gtattgctaa gcgcctgcag tggcaccgca ccggataatc
    10501 tgggcattcg tgagggccgc ctggccagct gcccggactc accgaactgc gtaaacagcc
    10561 aggccagcga tgcgcgacac gccattgagc cgctgccgct gcaaggctct gccgagcaaa
    10621 cccaggcacg cctgaaggcg ctgcttagcg aagagccgcg ggtgaatctg gtagaggagg
    10681 caccgggtta tctgcgtgcc gaattcagca gcaagctgat gcgctttgtc gatgatgtgg
    10741 agtttctgat tggagcggcc gccgtggatg tgcgctcggc gtcgcgcctg ggctataagg
    10801 atttcgacgt caaccgcgaa cgcattgagc atctgcgcca gcgccttggc gaatagcgca
    10861 gcccttgccc gcgctactcc tgcggatgga tcagcgccaa acgctcgtgc accacatcca
    10921 ccagcttgtc cggttggaat ttggagagga agttgtcgca accgaccttc ttcaccatgg
    10981 cttcgttgaa actgccggac aatgaggtgt gcagtacaac gtacaggtct ttcagacgtg
    11041 gatcgctacg gatttccgtg gtcaggcgat agccgtccat ttctggcatt tccgcatcgg
    11101 tgatgaccat cagcagcttt tcctgcatgt ccaccccgga gtcggcccac agctgcagca
    11161 ttttcagggc cttgagcccg tcacttgcaa cgtgggtacg aatctgcagc tgcgccatgg
    11221 tttccttcaa ctgggtcagg gcgacgttgg agtcgtccac cagcagcact tcacggccct
    11281 tggctttttc cagcagcgga tcgctgagct tttcggccga gaccttgctg ctcatcggcg
    11341 agatttccga cagccccttc tccacgtcga tcacctcgac gatctggtca tcgaccttgg
    11401 tgatcgccgt caggtaatgc tgccgtccgg caccaccggg cggcggcagg atcgactccc
    11461 aattgaggtt gagaatgcgg tccacgccac cgaccagaaa cgcctgcacc gagcggttgt
    11521 actcggtgac gataatggtg ctggtttcac tcggcacttg cgcgcgcatg ccaatcgctt
    11581 gcgacaggtc gatgaccggc aaggtttgac cgcgcaaact gaccaccccg cagacgaagg
    11641 gatgacgctg cggcatcaag gtcaacttag gcatctgcag gacttcctgc actttgaaca
    11701 cgttgatggc aaatagctgc cgaccggcca aacgaaacat taaaatttcg aggcggttag
    11761 cgccaaccaa ctgagtgcgt tgatttactg agtcgagaat gccggccatc atgcgctcct
    11821 gtttcacttt atcgatctag cctagctcaa atagccacag cttaatgtat cggccatcgg
    11881 tgctgcatct gaaggcgcat agcggtaagc catcatgcgc gaagcccgga gcagaggcca
    11941 accagagtgg ccattgacac attctaatcg cgcaacagtg gcgagtccgc cggcaaatgc
    12001 acccgcaagg cagccggcat ggccgcaaac cgcaagcggc tgctctccat cggctcaccg
    12061 tccaggttca tgtccaggcc ttccggggcc tcgatttcca gccagggcag acgggcattc
    12121 agggccacgc tttgcaagcc gttgatcccg ccagacagca gcgtgccgag ggtgccgacc
    12181 acatctgcgg gggcc
//
LOCUS       DASRUM010000062         6875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_697196, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000062 DASRUM010000000
VERSION     DASRUM010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697196"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFF22_04490"
     CDS             <1..262
                     /locus_tag="VFF22_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZX16113.1"
                     /translation="AGYAWPVIDLQLRYIRGAQFGQKIIVRADLVEWENRLKINYLIT
                     DAATGERMTRGSSVQVAVEIATREMQFASPKVLIDAVEKVLA"
     gene            289..924
                     /locus_tag="VFF22_04495"
     CDS             289..924
                     /locus_tag="VFF22_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HZX16114.1"
                     /translation="MKSGKRSPTVHRSRIASGLQLVILLLLASDAYAFDLDQLSAQLA
                     KPAVVRGPLIQEKHLRALPQPLTSRGQFVLSRDLGLLWQLQSPLKQDYRIDDLGIAKR
                     TPKGWQQQPGQDVAAQQSRLFLAVLKGDHSGLARDFELQLSGTSEAWQLRLVPNSLLL
                     KQIFSRIQIDGGALVQRIELHETQGDHSVLRLPESQAGDALTEQEQRDFAK"
     gene            914..3268
                     /locus_tag="VFF22_04500"
     CDS             914..3268
                     /locus_tag="VFF22_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16115.1"
                     /translation="MPSERWLPRGFLLLLAALLALAAWQWRNGPPVAADMLALLPAGS
                     GDALVQQAEQRMQEPLNRDLLLLIGHPQRDQAIALAQQVGEQWRGSERFAQVQWSVDS
                     DLAALRDYLRSNRLALLPAADRQLLLEQPQQLIEQRAAQLFDTFAGFSLLPIEQDWLG
                     LGVRAQQALNPGSRIQADLSSGALLLEEPGMTWALLRASAQGSAFDMQEPPLIATQVA
                     ATRAQIETAGGQLLAAGGVLYAAAGQAKATREISLIGGGATLGTLLLLLLAFRRVRVL
                     VSLLPIGMALLAGCTACVLVFGQINALTLVLGASLVGVAADYPQHYLSKSWSNAGGTD
                     GWTSWGALRATLPGLSLSLGTNLIGYLALAFTPFPALTQVALFSAAGLIAAYLCSVCL
                     LPAWLRGLRLSPSPQLLSFSQRLLAGRARLLAKTGSAPLLVLLLLFCAGGLWQLHTQN
                     DLRQWLGHEPELLAEAQRIAELTGQQPTSQFFLVRAANPQQLLERQAALSQRLDQAVQ
                     NGQLRDYRALSQLVAPDSQLQELRAAMNELPQHWQPLLDLGIPPAALQGELLTLQQNN
                     QASLEQALASPLGEAWRPLWLGAHSKNGADGVAGLVSLQGLSNSSELKPLVEGLPGVQ
                     LVDRISELNALFSATQLSAAELKLISSVAILLLLCLPFGLGGALRVVCLPLLAALAAL
                     ACLGWLGQPLTLFSLFGLLLITAIGVDYAILMRENIGGPAVSLLGTLLSALTSWLSFG
                     LLLISDTPAIANFGLAISLGLLFCFLLAPWAGTRQATVNQAQTT"
     gene            3265..4416
                     /locus_tag="VFF22_04505"
     CDS             3265..4416
                     /locus_tag="VFF22_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HZX16116.1"
                     /translation="MTIALAWLALLALFALVTWLGKRLRLIPIVSQLLLATLGLPLLM
                     LWTAPMGFGPSELLAPIWLEPLYGLAFCLLLGHILSDVVDLQLEPGSLQIALPSFFVP
                     FFCGLACAVWLLPEQSGLATIAVGLLFALTAIPVLFLYLQHLGYPVAKIRQLLQAAII
                     MDLLCWLIFGLAQGSSDPATLLWPLLAACLPLLLKLLPLRSALLNGSCFLALLLVMQQ
                     LHLNALVFGIAYLLLLTALRLPLRLPLPAGLLRWLQTWLAVPLILAFGLLQIDWHDAW
                     LGYSAWQFAALLLLPIISKLAGNWLGLSWAAGQLRAYAGQWRESLLLNTRGLTEIIFL
                     NLLLQQQIISPALYFALMLMGLISTLLPALAGAYPHAAKHEARSRYEVS"
     gene            4403..5647
                     /locus_tag="VFF22_04510"
     CDS             4403..5647
                     /locus_tag="VFF22_04510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZX16117.1"
                     /translation="MKSLEVEQRQVVVIGAGPSGAIAAALLKRNGHDVLVLERQRFPR
                     FSIGESLLSHCLDFVEEAGMLDAVRAAGFQTKHGAAFGWGERYTEFDFRDTFTKGQGS
                     TYQVLRADFDKLLADQAELQGVEIRYEEEIFAVDFSGECPRLSVRRLADGHEYQVECA
                     FVLDGSGYGRVLPRLLDLDTPSSFPVRQAVFTHVEDRSEGTGFDREKILITTHPTLRD
                     VWFWTIPFSNGRCSVGVVADATRYQGRPEDLDACLKQFIEETPSLHKVLKNALWDTPA
                     RTIGGYSANVKTLHGPGFALLGNAAEFLDPVFSSGVTIAMRSASMAAGLLHRQLSGET
                     VDWEADFAIPLKRGVDTFRVYVEGWYDGSFQDVIYFEKAQPEIRRMISSILAGYAWDE
                     KNPYVAEPRRRLRVLAELCATS"
     gene            5659..6387
                     /locus_tag="VFF22_04515"
     CDS             5659..6387
                     /locus_tag="VFF22_04515"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX16118.1"
                     /translation="MSNPAATYVEETRFGFWFLQSHVWQHHVLRVAINDLRGLFAGPL
                     PEAPVLLDAGCGQGKSFGLLAETFKPARIIGLDADPHSLDCSRAEAERLGLDVQLIAS
                     DCAAIDLPDASVDLLFCHQTFHHLVEQEQALAEFWRVLKPGGRLLFAESTQFYIDTWV
                     IRWLFRHPMHVQKSAEGYLDMLRGQGFEFATQNVSYPYLWWSRSKDFGLLERWGLLKP
                     KPVGQRNETLVNVVARKPLEASPV"
     gene            6384..>6875
                     /locus_tag="VFF22_04520"
     CDS             6384..>6875
                     /locus_tag="VFF22_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16119.1"
                     /translation="MKRIALLGLCLLLSACAARTPLPLTAPTFGAPLPAALRVERQQG
                     DDQQTWWLVVQAEQSALRWSLFDPLGVPLARQLLEDGQWRADGLLPPNSEAQELFAAL
                     LFALTPIDKLPASYAPDTWQQLADGQRRLNPHWLIDYRAPLDFTLSQGQVLSYRVYAL
                     ADEE"
BASE COUNT         1131 a         2294 c         2137 g         1313 t
ORIGIN      
        1 cgccggctac gcctggccgg tgatcgacct gcagctgcgc tatatccgtg gcgcgcagtt
       61 tggtcagaag atcatcgtgc gtgccgatct ggtggagtgg gaaaaccgcc tgaagatcaa
      121 ctacctgatc acggatgccg caaccggcga gcgcatgacc cgtggcagca gcgtgcaggt
      181 ggcggtggaa atcgctaccc gcgagatgca gtttgcttcg cccaaggtgc tgatcgacgc
      241 cgttgaaaag gtgctggcgt gaacgccctg cacaacctcg tagcccggat gaaatccggg
      301 aagcgttctc cgaccgtgca ccgctcccgg attgcatccg ggctacagct ggtgatcctg
      361 ctgcttctgg catccgatgc ttatgccttt gacctcgacc aactcagcgc ccagctggcc
      421 aagcccgccg tggtgcgtgg cccgcttatt caagaaaagc acctgcgcgc cctgccgcag
      481 ccgctgacca gccgtggcca gtttgtgttg agccgcgatc tcggcctgct ctggcagctg
      541 caaagcccgc tcaagcagga ctaccgcatc gacgacctgg gcatcgccaa acgcacgccc
      601 aaaggctggc aacagcagcc cggccaggat gtcgctgccc agcagagccg gttgtttctc
      661 gccgtgctca agggcgacca tagcggcctg gcgcgggatt tcgagctgca actgagcggc
      721 acgtctgaag cctggcaact gcgcctggtg ccgaactcgc tgttgctcaa acagattttc
      781 agccgcatcc aaatcgatgg cggcgcgctg gtgcagcgta tcgagctgca tgagacccag
      841 ggcgatcaca gcgtgctgcg cttgccggaa agccaggccg gcgatgcctt gactgagcag
      901 gagcaacgcg actttgccaa gtgaacgctg gctgccgcgc ggctttctgc tgctgctcgc
      961 cgccctgctg gcgcttgccg cctggcagtg gcgcaacggc ccgcccgtag cggcggatat
     1021 gctcgcgctg ttgccggccg gtagtggaga cgccctggtg cagcaggccg aacagcgtat
     1081 gcaggagccg ctcaatcgcg acctgttgct gttgatcggt cacccacagc gcgaccaggc
     1141 gattgccctg gcgcagcaag ttggcgagca gtggcgcggc agcgagcgct tcgcccaggt
     1201 gcagtggagc gtcgacagcg acctcgcggc gctgcgcgac tacctgcgca gcaaccgcct
     1261 ggccctacta cctgcggcag atcgccagct gctactggag caaccgcaac agttgatcga
     1321 acaacgcgcc gcccagctgt tcgacacctt cgccggcttt agcctgctgc ccatcgagca
     1381 ggactggctg ggccttggcg tacgcgcgca acaggcgctg aaccccggca gccgcatcca
     1441 ggccgacctg agcagcggcg cattgctgct ggaagagccg ggtatgacct gggccctgct
     1501 gcgtgccagc gcccagggca gcgccttcga tatgcaggag ccgccgctga ttgccacaca
     1561 ggtcgccgct acccgtgcac agatcgaaac agccggcggc caactgctgg cggccggtgg
     1621 cgtgttgtat gcggcagccg gccaggccaa ggccacccgc gagatcagtc tgattggcgg
     1681 cggcgctacg ctcggcaccc tgctgctgtt gctgctggcc ttccgccgcg tgcgcgtgct
     1741 ggtcagcctg ctgccgatcg gcatggcgct gctcgccggc tgcaccgcct gcgtgctggt
     1801 gtttggccag atcaacgccc tgactctggt attgggcgcc agcctggtcg gggtcgctgc
     1861 ggactatccg caacattact taagcaagag ctggagcaac gccgggggca cagacggctg
     1921 gaccagctgg ggcgccctgc gcgcgacctt gccgggcctg agcctgagcc tgggcaccaa
     1981 cctgatcggc tacctggcgc tggcctttac cccgtttccg gcgctgaccc aggtcgcgct
     2041 gttttccgct gctgggctga ttgccgctta tctgtgctcg gtatgcctgc tgccggcctg
     2101 gctccgcggc ctgcggctaa gcccatcgcc gcagctgctg agttttagcc aacgcctgct
     2161 ggccggccgg gcacgcttgc tggccaagac aggtagcgcg ccgctactcg tgctgttgct
     2221 gctgttctgc gccggcgggc tgtggcagct gcatacacaa aatgatctgc gccagtggct
     2281 cggccatgag ccggaattgc tggccgaagc ccagcgcatc gccgagctga ccggacaaca
     2341 gcccaccagc cagttctttc tggtgcgcgc cgctaaccca cagcagttgc tggagcgcca
     2401 ggcggcgcta agccagcgtc tggatcaggc agtgcagaac ggtcagttgc gcgactaccg
     2461 cgccctgagc caactggtcg ctcccgacag tcagctgcag gagttgcgcg ctgcaatgaa
     2521 cgagctgccg cagcactggc agcccctgct cgacctgggc atcccgccgg ccgcgctgca
     2581 aggcgaactg ctgacgctgc aacagaacaa ccaggcgagc cttgagcaag ccctggccag
     2641 cccccttggc gaggcctggc gaccgctgtg gctgggcgct catagcaaga acggggcaga
     2701 tggcgtggca gggctggtga gcctgcaagg actgagcaac agcagcgaac tcaagccgct
     2761 ggtcgaaggt ttaccggggg tgcagctggt ggatcgaatc agcgaactca atgcgctatt
     2821 cagcgccacc caacttagcg ccgcagaatt aaaactcatc tccagcgtgg cgatcctctt
     2881 gctgctgtgc ctgccgttcg gcctcggcgg cgcgttgcgg gtggtctgcc tgccgctact
     2941 cgcagcgctc gcagcgctgg cctgcctcgg ctggttaggc cagccactga ccctgttcag
     3001 cctgttcggc ctgctgttga tcaccgccat cggtgtcgat tacgccattc tgatgcgcga
     3061 aaacatcggc ggaccggcag tcagcctgct cggcaccctg ctctcggcgc tgaccagttg
     3121 gctgtcgttc ggcctgctgc tgatcagcga caccccggcg attgccaact tcggcctggc
     3181 gatcagcctt ggcctgctgt tctgcttcct gctcgcgcca tgggctggca ctcggcaggc
     3241 cacagttaac caggcgcaaa cgacatgacc atcgccctgg cctggttggc gctgctcgca
     3301 ctctttgctc tggtgacctg gctgggcaaa cgcctgcgcc tgatcccgat tgtcagccaa
     3361 ttgctgctgg ccaccctggg cctgccgctg ctgatgttgt ggactgcccc aatggggttc
     3421 ggccccagcg aactgctcgc cccgatctgg ctggagcctt tgtatggcct ggccttttgt
     3481 ctgctactgg ggcatatcct cagtgatgtg gttgatctac agctggagcc cggcagcctg
     3541 cagatcgccc tgccaagttt ctttgtgccg ttcttctgcg gcctggcctg cgcggtctgg
     3601 ctgctgccag agcagagcgg cctggccact attgccgtgg gcctgctgtt tgccctgacc
     3661 gccattccgg tgctgtttct ctacctgcag cacctcggct acccggtggc caagattcgc
     3721 cagctgctgc aggcggcgat catcatggat ttactctgct ggctgatctt cggcctggcc
     3781 cagggcagca gcgacccagc caccctgctc tggccgctgc tggccgcctg cctgccgctg
     3841 ctgttgaagc tgctgccgct gcgctcggca ctgctcaatg gcagctgctt tctcgctctg
     3901 ctgctggtga tgcagcaact gcacctgaat gccctggtat tcggcatcgc ctacctgctg
     3961 ctgctcactg ccctccgcct gcctttacgc ctgccgctac ctgccggcct gctgcgctgg
     4021 ctgcaaacct ggctggcggt gccgctgatt ctggccttcg gcctgctgca gatcgactgg
     4081 cacgacgcct ggctggggta ttctgcctgg cagtttgcgg cgctattgct gctgccgatc
     4141 atcagcaaac tggccggtaa ctggctgggc ctgagctggg ccgccggaca actgcgcgcc
     4201 tatgcggggc agtggcgcga aagcctgctg ttgaataccc gtggcctgac tgaaatcatc
     4261 tttctcaatt tgctgctgca acaacagata atcagcccgg cactgtattt cgccctgatg
     4321 ctgatgggcc tgatctcgac cctgctaccg gccctcgccg gtgcttaccc acacgccgcc
     4381 aagcatgagg ccagaagccg ctatgaagtc tcttgaagtc gaacaacgcc aggtcgtagt
     4441 gataggtgcc ggcccgtccg gggcgattgc cgcggcgctg ctcaagcgca acggccatga
     4501 cgtactggtg ctggagcgcc agcgcttccc acgcttctcg attggcgaaa gcctgctgtc
     4561 gcactgcctg gacttcgtcg aagaagccgg gatgctcgac gccgtgcgtg ctgcgggctt
     4621 ccagaccaaa catggcgcgg cctttggctg gggcgagcgg tataccgaat tcgatttccg
     4681 cgataccttc accaaaggtc agggttcaac ctatcaggtg ctgcgcgccg acttcgacaa
     4741 gctgctggcc gaccaggccg aattgcaggg tgtggaaatt cgctatgaag aggaaatctt
     4801 cgccgtcgat ttcagcggcg aatgcccgcg cctgagcgtg cggcgcttgg ccgacggcca
     4861 cgaataccag gtcgaatgcg ccttcgtcct cgacggcagc ggctacggcc gcgtgctgcc
     4921 gcgcctgctc gacctcgaca cgccgtcgag cttcccggtg cgccaggcgg tattcaccca
     4981 cgtcgaagac cgcagcgaag gcactggctt cgaccgcgag aagattctta tcaccaccca
     5041 cccgaccctg cgcgatgtgt ggttctggac catcccgttc agcaacggcc gctgctcggt
     5101 cggcgtggtg gccgatgcca cccgctatca gggtcgccca gaagacttgg acgcctgcct
     5161 taagcaattt attgaagaga cgcccagcct gcacaaggtg ctgaagaatg ctctgtggga
     5221 caccccggcg cgcaccatcg gtggctactc ggccaacgtg aaaaccctgc acggtccggg
     5281 ctttgccctg ctcggcaacg ccgccgaatt cctcgacccg gtgttcagct ccggggtgac
     5341 catcgccatg cgttcggcga gcatggccgc cggcctgttg caccgccaac taagtggcga
     5401 gacggtggac tgggaagccg actttgctat cccgcttaag cgcggcgtcg acaccttccg
     5461 cgtgtatgtc gagggttggt acgacggcag cttccaggac gtaatctatt tcgagaaagc
     5521 ccagccggaa atccgccgca tgatcagctc gatcctcgcc ggctacgcct gggatgaaaa
     5581 gaacccctat gtcgccgaac cgcgccgccg cctgcgggtg ctcgccgaac tctgcgccac
     5641 tagttaataa ggatatttat gagcaacccg gccgccacct acgtcgagga aacccgcttc
     5701 ggtttctggt ttttgcagag ccatgtctgg caacaccatg tgctgcgcgt ggcgatcaat
     5761 gacctgcgcg gcctgttcgc cggtccgctg ccagaagcgc cagtgttgct ggatgccggc
     5821 tgcggtcagg gcaagtcctt cggtctgctt gccgagacgt tcaaaccggc gcggataatc
     5881 ggcctggatg ccgacccgca cagcctcgat tgttcccgtg ccgaggcgga gcgcctgggc
     5941 ctggatgtgc aactgatcgc cagcgactgc gccgccatag acctaccgga tgccagcgtc
     6001 gacctgctgt tttgccacca gaccttccat catctggtcg agcaggagca ggccctggcc
     6061 gaattctggc gcgtgctcaa accgggcggg cgactgctgt tcgccgaatc cacccagttc
     6121 tatatcgata cctgggtgat ccgctggctg ttccgccacc ccatgcatgt gcagaaaagc
     6181 gccgaaggct atctggatat gctgcgcggc cagggcttcg agttcgcaac ccagaatgtt
     6241 tcctacccct atctgtggtg gagccgctcc aaggacttcg gcctgctgga acgctggggg
     6301 ctgctaaagc ccaagccggt cggtcagcgc aatgaaaccc tggtcaatgt ggtggcacgc
     6361 aagccactcg aggcaagccc tgtatgaagc gcatcgcctt gctcggtctc tgcctgctgc
     6421 tgagcgcctg cgcagcacgc acgccgctgc cgctgactgc accaaccttc ggcgcgccac
     6481 tgcccgccgc gctgcgcgtc gaacgccaac aaggcgacga tcaacagacc tggtggctgg
     6541 tggtgcaggc cgagcaatcg gcactgcgct ggtcgctgtt cgatccactc ggcgtgcctc
     6601 tggcgcggca actgctggag gatggccagt ggcgcgccga tggcctgctg ccacccaata
     6661 gcgaagcgca ggaactgttc gccgccctgc tgttcgctct caccccgatc gacaagctgc
     6721 ccgccagcta tgcgccggac acctggcagc aactggccga cggtcagcgc cggcttaatc
     6781 cgcactggct gatcgactac cgtgcgccgc tggatttcac cctgagccag ggccaggtac
     6841 tgagttaccg ggtctatgcc ctcgccgacg aagag
//
LOCUS       DASRUM010000063         7514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1709211, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000063 DASRUM010000000
VERSION     DASRUM010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7514
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709211"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /gene="murD"
                     /locus_tag="VFF22_04525"
     CDS             complement(<1..310)
                     /gene="murD"
                     /locus_tag="VFF22_04525"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253104.1"
                     /note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
                     involved in peptidoglycan biosynthesis; cytoplasmic;
                     catalyzes the addition of glutamate to the nucleotide
                     precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
                     formation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZX16120.1"
                     /translation="MSLIASDHFRIVVGLGKSGMSLVRFLARQGLPFAVAYSRANPPE
                     LATLRAEFRQVDVRCGELDVAFLCRANELLVSPGLALATPALQQAAARGVKMSGDIEL
                     F"
     gene            complement(318..1400)
                     /gene="mraY"
                     /locus_tag="VFF22_04530"
     CDS             complement(318..1400)
                     /gene="mraY"
                     /locus_tag="VFF22_04530"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZX16121.1"
                     /translation="MLLLLAEYLQQFHKGFAVFQYLTLRGILGVLTALALSLWLGPWM
                     IRTLQIRQIGQSVRNDGPQSHLSKKGTPTMGGALILSAIAVSTLLWADLTNRYVWTVL
                     AVTLLFGAIGWVDDYRKVIEKNSKGLPSRWKYFWQSVFGLGAAFFLYMTAQSPVETTL
                     ILPMLKDFSLELGLGFVVLTYFVIVGSSNAVNLTDGLDGLAILPTVMVGGALGIFCYL
                     SGNVKFAEYLFIPYVPGAGELIVFCAALVGAGLGFLWFNTYPAQVFMGDVGALALGAA
                     LGTIAVIVRQEVVLFIMGGIFVVETLSVIIQVASFKLTGKRVFRMAPIHHHFELKGWP
                     EPRVIVRFWIITVILVLIGLATLKLR"
     gene            complement(1400..2770)
                     /gene="murF"
                     /locus_tag="VFF22_04535"
     CDS             complement(1400..2770)
                     /gene="murF"
                     /locus_tag="VFF22_04535"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HZX16122.1"
                     /translation="MLKALRLSEVAAPLAARVIGADVAFSAVSSDSRAIQPGQLFVAL
                     AGPRFDGHDYLAEVAGKGAVAALVEREVADAPLPQLLVADTRLALGQLGALNRQAFNA
                     PLVAITGSSGKTTVKEMLASILREGLQGPVLATRGNLNNDLGAPLTLLELAPEHVAAV
                     IELGANHIGEIAYTVGLTQPQVAIINNAGNAHVGEFGGPEKIVQAKGEILEGLSDSGV
                     AVLNLDDSAFAIWQQRAQGHAISSFALRNSAADVYASDLARDARGCSAFTLHCASGQA
                     RIQLNLLGEHNVANALAAAAAAQALGITPAAIKAGLESLQPVKGRAVAQIAANGLHVI
                     DDSYNANPASVCAAIDILAGFPGRRVLVLGDIGELGEWAEQGHRQVGSYAVGKVDALY
                     AVGPLTAYAVAEFGAAARHFADQASLIAALQSEQGDTTLLIKGSRSAAMEKVVAALCG
                     TSGEIH"
     gene            complement(2763..4226)
                     /locus_tag="VFF22_04540"
     CDS             complement(2763..4226)
                     /locus_tag="VFF22_04540"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HZX16123.1"
                     /translation="MPMPLNQLLPEAASSTLIRELTLDSRKVRPGDLFLAVPGTQQDG
                     RVHIADAVARGAAAVAYEAEGAAVMTAHSAELVALKGLANQLSAIAGRFYGEPSRGLH
                     LIGITGTNGKTSVSQLLAQALDLLGERCGIVGTLGNGFYGALEQGRHTTPDPIGVQAT
                     LADMKNAGARAVAMEVSSHGLHQGRVAALAFDVAVLTNLSRDHLDYHGSMQAYAEAKA
                     ALFAWPSLRCRVLNLDDAFGRELAAAEHESRLISYSLIDADAYLYCRDAQFDEQGVRA
                     TLVTPRGEGSLRSSLLGRFNLSNLLAVVGALLGLDYPLDEILKVLPQLQGPIGRMQRL
                     GGVKQPLVVVDYAHTPDALEKVLEALRPHVKGRLLCLFGCGGDRDSGKRPLMAAVVER
                     LADGVLVTDDNPRTEVPAQIFSDIRAGFASPDSVQFVHGRGQAIAQLIAAASADDVLV
                     LAGKGHEDYQEINGVRQPFSDLVEAANALAAWEVAHA"
     gene            complement(4226..5965)
                     /locus_tag="VFF22_04545"
     CDS             complement(4226..5965)
                     /locus_tag="VFF22_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZX16124.1"
                     /translation="MGLEGALYPWRFRLVLLLLALMVGAIAWRIVDLQVFDQSFLQAH
                     GDARSVRHIPIPAHRGLITDRNGEPLAVSTPVTTLWANGKELQSGKAKWNELAAALGQ
                     EPKAFNVRLQANAEREFMYLVRGLTPEQGQRILDLKVPGVYGIEEFRRFYPAGEVAAH
                     VVGFTDIDDRGREGMELAFEDWLAGVPGKRQVLKDRRGRLIKDVQVAQNAKAGKALAL
                     SIDLRLQYLAHRELRNAVTEFGAKAGSLVLLDVQTGEVLAMVNQPTYNPNNRRNLQPA
                     AMRNRAMIDVFEPGSTVKPFSIAAALGSGRWTPEMTVDTAPGSLRIGRYTIRDVSRGG
                     VLNLTQILMKSSNVGISKVAFDIGAEPIYAVMQQVGLGQDTGLSFPGERVGNLPNYRV
                     WKQAETATLAYGYGLSVTAVQLAHAYSVLANDGKNVNLSMVRLDRQPVTTQVIDEQIA
                     KTVLGMLQSVVEEPGGGGARAQVPGYHVGGKSGTARKASTDSKGYVDKAYRSFFAGVA
                     PIDNPRIAAVIMIDEPREGGGYYGGLVAAPVFGKVMAGALRLMNVAPDNLPEPEPAKV
                     ETAAVDPLKGGRI"
     gene            complement(5965..6258)
                     /gene="ftsL"
                     /locus_tag="VFF22_04550"
     CDS             complement(5965..6258)
                     /gene="ftsL"
                     /locus_tag="VFF22_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003377628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HZX16125.1"
                     /translation="MSAPFAKPLPGGSLLMLLLFVAVLVSAVGVSYSAHWNRQLLNEL
                     YAELSVRDKSQAEWGRLILEQSTWTAHNRIETLATERLSMRIPDAAEVRMVAP"
     gene            complement(6255..7196)
                     /gene="rsmH"
                     /locus_tag="VFF22_04555"
     CDS             complement(6255..7196)
                     /gene="rsmH"
                     /locus_tag="VFF22_04555"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HZX16126.1"
                     /translation="MTSNFRHITVLLDEAVEGLAVRANGCYLDGTFGRGGHSRLILEK
                     LGPDGRLLGFDKDPLAIATGNALAAEDGRFVVVQRSFAELGAELAQRALGGQVSGILL
                     DLGVSSPQLDDAERGFSFMNDGPLDMRMNPDAGVSAADFIASAAEEEIARVFKEYGEE
                     RFAKRMARAVVLRRAEQPFTRTADLAQVLTVANPAWEKGKNPATRAFQGLRIYINNEL
                     GDLESGLDAALENLEVGGRLVVISFHSLEDRIVKLFMRKHAKGEMDKLPRDLPIIPKA
                     FEPRLKLIGKPVFASEAELAANPRSRSAVMRIAEKVR"
     gene            complement(7199..>7514)
                     /locus_tag="VFF22_04560"
     CDS             complement(7199..>7514)
                     /locus_tag="VFF22_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003103101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HZX16127.1"
                     /translation="YPLSEWELIEAKLRELASFREENRRLQRLLIGNAVDLELDASGR
                     FLVPPRLREYARLDKRAMLVGQLNKFQLWDEDAWNAVADADLAAIKQPGALSDDLRDL
                     IL"
BASE COUNT         1509 a         2433 c         2282 g         1290 t
ORIGIN      
        1 caaacaattc gatatcgccg gacattttca cgccacgcgc ggccgcctgc tgcaacgctg
       61 gggtcgccag ggccaaacca gggctgacca gcagctcgtt ggcgcggcag agaaacgcca
      121 catccagctc gccacaacgc acgtccacct gccggaactc ggcacgcaag gtggccagct
      181 ccggcgggtt ggcacggctg taggccacgg caaagggcag gccctggcgc gcgaggaaac
      241 gcaccaggga catgccgctc ttgccgaggc cgacaacgat gcggaagtgg tcagacgcga
      301 tcaggctcat acaatcctca gcgcagtttc agggtggcaa ggccgatcag caccagaatc
      361 acggtgatga tccagaagcg cacgatcacg cgcggctcgg gccagccttt cagttcaaaa
      421 tggtgatgga tcggcgccat gcggaacacg cgcttgccgg tcagcttgaa gctggcgacc
      481 tggataatca ccgacagggt ttccaccacg aaaatgccgc ccatgatgaa cagcaccact
      541 tcctgacgga cgatcacggc gatggtgccc agggccgcac ccagcgccaa cgcgccaacg
      601 tcacccataa acacttgcgc cgggtaggtg ttaaaccaga ggaaaccgag gcccgcgcca
      661 accagcgcgg cgcagaacac aatcagctca cccgcaccag gcacgtaggg aataaacagg
      721 tattcggcga acttcacgtt gcccgacagg tagcagaaga tccccagcgc gccgccgacc
      781 atcacggtag gcagaatggc caggccatcg aggccgtcag tcaggtttac cgcgttgctc
      841 gaaccgacga taacgaagta ggtcagcacc acgaagccca gacccagctc caggctgaag
      901 tccttgagca tcggcagaat cagggtggtt tccaccgggc tctgcgcagt catataaagg
      961 aagaacgctg caccgaggcc gaacaccgac tgccagaagt atttccagcg gctcggcagg
     1021 cccttggagt tcttctcgat caccttgcgg tagtcatcga cccagccgat ggcgccgaac
     1081 agcagggtta cggccagtac ggtccacacg tagcggttgg tcaggtcagc ccagagcagg
     1141 gtgctgacgg cgatggccga gagaatcagc gcaccgccca tggttggcgt gcccttcttc
     1201 gacaggtgcg actgcgggcc gtcgttgcgt accgactggc cgatctggcg gatctgcagg
     1261 gtgcggatca tccatgggcc gagccacagc gacagggcca gcgcggtcaa cacgccgagg
     1321 atgccgcgca gtgtcaggta ctgaaacact gcgaaaccct tgtggaactg ttgcaggtat
     1381 tccgccagca gcagcagcat tagtgaatct ccccggatgt gccacacagc gccgcgacga
     1441 ctttttccat cgccgcactg cgtgaacctt taattagaag ggtggtgtcg ccctgctcgc
     1501 tttgcagcgc tgcaatcagg ctggcctgat cggcaaaatg ccgagctgcc gcgccaaact
     1561 cggcaaccgc gtaggccgtc agtgggccca cggcatacag cgcatcaacc ttgcctacgg
     1621 catagctgcc aacctggcga tgaccctgct cggcccactc gcccagctca ccgatatcgc
     1681 ccagcaccag cacgcggcgg ccagggaaac cggccaggat atcgatggct gcgcaaacgg
     1741 atgccgggtt ggcgttgtag ctgtcgtcga taacgtgcag gccgttcgct gcgatctgcg
     1801 caacggcgcg gcctttgacc ggctgcaggc tttccagccc ggccttgatc gcagcaggtg
     1861 taatccccag cgcctgagca gccgccgccg cagccagggc attagcgacg ttgtgctcgc
     1921 cgagcaggtt gagctgaata cgcgcctgac cactggcgca atgcagggta aaggctgagc
     1981 agccacgggc gtcgcgagcg agatcgctgg cataaacgtc agcggcagaa ttacgcagag
     2041 cgaagctgct gattgcatgg ccctgggcgc gttgctgcca gatggcaaag gcgctgtcgt
     2101 caaggttgag cacggccaca ccgctgtcgc tcaagccttc gaggatttcg cctttggcct
     2161 gaacaatctt ttccgggccg ccgaactcgc cgacatgagc gttaccggca ttgttaatga
     2221 tggccacctg cggctgggtc aagccgacgg tgtaggcgat ttcgccgata tggttggcac
     2281 ccagttcgat cactgcagcg acatgctcag gggccaactc cagcaaggtc agcggcgcac
     2341 cgaggtcgtt attcaggttg ccacgagtcg ccagcaccgg gccttgcagg ccttcacgca
     2401 gaatactggc aagcatttcc ttaacggtgg tcttgccgct ggagccggta atagcgacca
     2461 gtggcgcgtt aaacgcctga cgattgagcg cgcccagttg gccgagggcc agacgggtat
     2521 cggcgacgag cagttgcggc agcggcgcat ccgccacttc acgctcaacc agggcagcca
     2581 ctgcgccttt gccggcgact tcagccagat agtcatggcc atcgaagcgc gggccggcca
     2641 gggcgacaaa caactgaccc ggctgaatcg cccggctgtc gctgctgacg gcgctaaacg
     2701 ccacgtccgc accgatcacc cgggcagcca atggcgcagc cacttcgctc aaccgcagcg
     2761 ccttaagcat gcgccacctc ccatgccgcc aaagcgttgg ccgcttcgac cagatcggag
     2821 aacggctgac gtacgccgtt gatttcctga tagtcctcat gacccttgcc ggccagtacc
     2881 agcacgtcat cggcactggc agcggcaatc agctgagcaa tcgcctggcc gcggccatga
     2941 acgaactgca ccgaatccgg gctggcaaag ccggcgcgaa tatcgctgaa aatctgtgcg
     3001 ggaacttccg tacgcgggtt gtcatcggtc accagcacgc catcggccag gcgctcaacc
     3061 acggcggcca tcagcggacg cttgccgctg tcgcgatcac caccacagcc gaacaggcat
     3121 agcaggcggc ctttaacgtg cgggcgcagg gcttcgagga ctttttccag ggcgtccgga
     3181 gtgtgtgcgt aatccaccac caccagcggt tgcttgacgc cacccagacg ttgcatacga
     3241 ccaatcgggc cctgcagctg cggcagcacc ttgagaattt catccagcgg gtaatccagg
     3301 ccaagcagcg cgccgaccac tgccagcagg ttgctcaggt tgaagcggcc gagcagcgaa
     3361 ctgcgcagac tgccttcacc acgcggagtc accagcgtgg cgcgcacgcc ctgctcgtcg
     3421 aactgcgcgt cgcggcaata caggtacgcg tcggcgtcga tcaggctgta gctgatcagg
     3481 cgcgactcgt gttcggcagc tgccagctca cggccaaagg cgtcgtccag gttgagcacg
     3541 cggcaacgca ggctcggcca agcgaacagc gcagctttcg cctcggcgta ggcctgcatc
     3601 gaaccgtggt aatcaagatg atcgcgcgac aggttggtca gcaccgccac atcaaacgcc
     3661 agagcggcaa cgcggccctg atgcagaccg tgggacgaga cttccatggc cacggcgcga
     3721 gcgccggcgt ttttcatgtc agccagagtc gcctgcacgc cgatcgggtc gggcgtggtg
     3781 tggcggcctt gctccagcgc gccgtaaaag ccattgccca gggtgccaac gatgccacag
     3841 cgctcgccaa gcagatctag cgcctgagcc agcaactggc tgacgctggt tttgccgttg
     3901 gtgccggtga tgccgatcag gtgcagacca cggcttggct cgccatagaa gcgtccggca
     3961 atcgccgaca gctgattggc caggcctttg agcgcgacca gttcggcact gtgcgcagtc
     4021 atcacggccg cgccttcggc ttcataggcc acggcggcgg cgccacgggc aaccgcatcc
     4081 gcgatatgca cgcggccgtc ctgctgggtg cctggcacgg caagaaacag atcgcccgga
     4141 cgcaccttgc ggctatccag ggtcaattca cgaatcaggg tggagctggc ggcttccggc
     4201 agcaactgat taaggggcat aggcatcaga tgcgccctcc tttaagggga tcaacagctg
     4261 cggtttcaac cttggctggc tcgggctccg gcaggttatc cggggcgacg ttcatcagcc
     4321 gcagcgcacc agccataacc ttgccaaaca ccggcgcggc gaccaggccg ccgtagtaac
     4381 cgccgccctc tctgggctca tcaatcatga tgaccgcggc aatacgtggg ttatcgatag
     4441 gcgctacacc ggcgaagaac gagcgatacg ccttgtcgac atagccttta ctgtcggtcg
     4501 aagccttacg cgccgtgccg cttttgccac cgacgtggta gcccggcacc tgggctcgcg
     4561 caccgccgcc gcccggctct tcaaccacgg actggagcat gccaagcacg gtcttggcga
     4621 tctgctcatc gatcacctgg gtagtaaccg gctggcgatc caggcgcacc atcgacaggt
     4681 ttacgttctt gccgtcgttg gccagcaccg agtaggcgtg cgccagttgc acggcggtaa
     4741 ccgacaggcc gtagccatag gccagagtgg cggtctcggc ctgtttccag acgcggtagt
     4801 tgggcagatt accgacgcgc tcgccaggga agctcaggcc ggtgtcttgg cccaaaccga
     4861 cttgctgcat aacggcgtaa atcggctctg cgccgatatc gaaggccacc ttgctgatgc
     4921 cgacgttgct cgacttcatc agaatctggg tcaggttgag cacgccacca cgcgatacgt
     4981 cgcggatggt gtaacggccg atgcgcagac tgccaggggc agtatccacg gtcatttccg
     5041 gggtccagcg gccagagccc agggcagcgg cgatagagaa cggcttgacg gtcgaacctg
     5101 gctcgaacac atcaatcatc gcccgattac gcatggcggc cggttgcagg ttgcgacgat
     5161 tgttcgggtt gtaggtcggc tggttgacca tggccagcac ttcgccagtc tgtacgtcga
     5221 gcagcaccag actgccggcc ttggcgccga actcggtgac ggcgttacgc agctcgcggt
     5281 gcgccaggta ttgcagacgc aggtcaatcg acaatgccaa ggccttaccg gccttggcat
     5341 tctgtgcaac ctggacgtcc ttgattagcc gcccacgccg atccttgagc acctggcgct
     5401 tgccgggcac gccggccagc cagtcctcga aggccaactc catgccttca cggccacgat
     5461 catcaatgtc ggtaaagccc accacatggg cagccacctc accggcagga tagaagcggc
     5521 ggaactcttc gatgccgtac acgccgggca ctttcaggtc gagaatgcgc tgaccctgct
     5581 caggcgtcag gccgcgcacc aggtacataa attcgcgctc ggcattggcc tgcaagcgca
     5641 cgttgaaggc tttgggctcc tgacccaggg cggccgccag ttcgttccac ttggccttgc
     5701 cgctctgcag ctctttgccg ttggcccaca gagtggtcac cggagtgctg acggccagcg
     5761 gctcgccatt gcggtcggtg atcaagccac ggtgcgccgg aatcggaata tggcgcacgc
     5821 tgcgcgcatc gccatgggcc tgcaggaagc tctggtcgaa cacttgcagg tcgacaatac
     5881 gccaggcaat cgcgcccacc atcagtgcca acagcagtag caccaggcgg aaacgccagg
     5941 ggtagagtgc gccctcgagc cccatcatgg cgccaccatg cgcacttcgg cggcatccgg
     6001 aatacgcatg ctcaggcgct cagtggccag ggtttcgata cggttgtgcg ccgtccaggt
     6061 gctttgctcg aggatcagcc gcccccactc agcctgcgac ttgtcgcgca cgctcagttc
     6121 ggcatacagc tcattgagca gctgacggtt ccagtgcgcg ctgtaggaaa ctcccaccgc
     6181 agacaccagt acagccacaa acaacagcag catcagcaga ctgccgccgg gcaatggctt
     6241 ggcgaagggc gcgctcatcg cactttctcg gcaatgcgca tcacggcgct gcgcgaacgc
     6301 ggattagccg caagctcggc ctctgaggcg aataccggct tgccgatcaa tttcaggcgc
     6361 ggctcgaaag cttttgggat gatcggcaga tcacgcggca gcttgtccat ctcacccttg
     6421 gcatgcttgc gcataaagag tttgacgatg cggtcttcca gcgagtggaa gctgatcacc
     6481 accagacgac cgcccacttc gaggttttcc agagctgcgt cgaggccgct ttccagatca
     6541 cccaattcgt tgttgatata gatacgcagg ccctggaagg cgcgagtcgc cggattcttg
     6601 cccttttccc aggccggatt ggccacagtc aacacctggg ccaggtcggc ggtgcgggta
     6661 aacggctgtt cggcgcggcg cagtaccacg gcacgggcca tgcgtttggc gaagcgctct
     6721 tcgccgtact ccttgaacac ccgggcaatt tcttcttcag ccgcactggc aatgaagtct
     6781 gcagcgctga cgcccgcatc cggattcatg cgcatatcca gcgggccgtc attcataaag
     6841 ctgaagccgc gctcggcgtc atccagctgc ggcgacgaca caccaagatc cagcagaatg
     6901 ccgctaacct ggccgcccag cgcgcgctgc gcaagctcgg cgcccagctc ggcaaagctg
     6961 cgctgcacaa cgacaaagcg gccgtcttcg gccgccagcg cattccccgt cgcgattgcc
     7021 aggggatcct tgtcaaaccc cagcaagcga ccgtctggcc cgagcttttc caggatcagc
     7081 cggctatgcc cgccgcgacc aaaagtgcca tccagatagc aaccattcgc gcgcacagcc
     7141 agcccttcga cagcctcgtc gagtagcacg gtgatgtggc ggaaattgct ggtcatggtc
     7201 acaggatcag gtcacgtagg tcgtcggaca gagcacccgg ttgcttgatc gcggccagat
     7261 cggcatcggc cacggcattc caggcatctt catcccacag ttggaattta ttgagttggc
     7321 cgaccaacat cgcgcgcttg tccagcctgg cgtactcgcg cagacgcggc ggaaccagga
     7381 aacgaccact ggcatccagc tcaaggtcaa cagcgttgcc gatcagcaaa cgctgcaggc
     7441 ggcggttttc ttcacggaag gaggcgagct cgcggagctt ggcttcaatc agctcccact
     7501 cggacagtgg atag
//
LOCUS       DASRUM010000064         5582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1542178, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000064 DASRUM010000000
VERSION     DASRUM010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542178"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /gene="tpiA"
                     /locus_tag="VFF22_04565"
     CDS             complement(<1..543)
                     /gene="tpiA"
                     /locus_tag="VFF22_04565"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019372521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZX16128.1"
                     /translation="MRRPMVAGNWKMHGTRASVVELINGLNQQVLPADVDVAVFPSCL
                     HLGQVVDGLAGKAVAVGAQDCATEAQQGALTGEVAASQLHDARCSLVLVGHSERRSLL
                     GESDEVVTRKFAAAQSSGLLPVLCVGETLAQREAGRTLEVVAAQLSAVTDALGVEVFA
                     AAVIAYEPVWAIGTGLTASPA"
     gene            complement(609..1946)
                     /gene="glmM"
                     /locus_tag="VFF22_04570"
     CDS             complement(609..1946)
                     /gene="glmM"
                     /locus_tag="VFF22_04570"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZX16129.1"
                     /translation="MGRKYFGTDGIRGRVGDFPITPEFMLKLGWAAGMAFRKQGKCRI
                     LIGKDTRISGYMFESALEAGLSAAGADVMLLGPMPTPAIAYLTRTFHAEAGIVISASH
                     NPHHDNGIKFFSGQGTKLPDEIELMIEELLDQPMTVVDSEQLGKVSRINDAAGRYIEF
                     CKSSVPTSTDFAGLKIVVDCAHGAAYKVAPSVFRELGAQVTVLSAQPNGLNINDDCGS
                     THVEALQAEVLAQQADLGIAFDGDADRVLMVDHSGAVVDGDELLFIIARDLLERGKLQ
                     GGVVGTLMSNLGLELALADLGIPFVRAKVGDRYVIAELLSRNWQLGGENSGHLVCFQH
                     TTTGDAIIAALQVLMALKRREQTLGEARLGMQKCPQVLVNVRFAGGVDPLEHPAVQQA
                     SARVIEQMAGRGRVLLRKSGTEPLVRVMVEGDDEGQVRGYADELAKVVADVCA"
     gene            complement(1965..2816)
                     /gene="folP"
                     /locus_tag="VFF22_04575"
     CDS             complement(1965..2816)
                     /gene="folP"
                     /locus_tag="VFF22_04575"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZX16130.1"
                     /translation="MSVAPTFDRLPCGSRFLDLSRPQVMGILNVTPDSFSDGGRYGQR
                     DAALRHAEAMLQAGATLIDVGGESTRPGARAVSPVEELERVAPVVEAIARELDVIISV
                     DTSTPAVIRETSRLGAGLINDVRSLQRDGALDAAADSGLPVCLMHMRGEPTTMQQNPQ
                     YPDVVAEVREFLLERLTACAAAGIGTERVILDPGFGFAKTLEHNLSLFKHLAALHALG
                     RPLLVGVSRKSMIGKVLGHEVGERLYGSLALAALALSKGAHILRVHDVAQTVDVVRMI
                     AAVEAAE"
     gene            complement(2825..4741)
                     /gene="ftsH"
                     /locus_tag="VFF22_04580"
     CDS             complement(2825..4741)
                     /gene="ftsH"
                     /locus_tag="VFF22_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZX16131.1"
                     /translation="MAKNLVLWLIIAAVLVTVMNNFSSPTESNKLNYSQFIQQVQDGG
                     VKRVTVDGFIISGTRTDGSSFETVRPAIQDNGLIKDLMDNNVEIVGKQPEQQSIWTQL
                     LVASFPILVIIAVFMFFMRQMQGGAGGKGGPMSFGKSKARLLSEDQVKTTFADVAGCD
                     EAKEEVSELVEFLRDPGKFQRLGGRIPRGVLMVGSPGTGKTLLAKAVAGEAKVPFFTI
                     SGSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRHRGAGMGGGHDEREQT
                     LNQLLVEMDGFEMNDGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDIRGREQILK
                     VHMRKVPMGEDVAPAVIARGTPGFSGADLANLVNEASLFAARAGKRVVEMKEFELAKD
                     KIMMGAERKTMVMSDKEKLNTAFHEAGHAIVGRLVPEHDPVYKVSIIPRGRALGVTMF
                     LPEEDRYSLSKRALTSQICSLFGGRIAEEMTLGFDGVTTGASNDIMRATQLAKNMVTK
                     WGLSEKLGPMMYAEEEGEVFLGRSMGSNQSNVSADTAKVIDQEVRSIIDGCYSTARRL
                     LEENRDKLDAMAQALMKYETIDAEQIEDIMNGLAPREPKGWQGGDDSNGTPTAPVEVV
                     ESPEKPIGGPAAEL"
     gene            complement(4923..5546)
                     /gene="rlmE"
                     /locus_tag="VFF22_04585"
     CDS             complement(4923..5546)
                     /gene="rlmE"
                     /locus_tag="VFF22_04585"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HZX16132.1"
                     /translation="MARSKTSQGWLKEHFNDPYVKMAQKDGYRSRASYKLLEIQEKDR
                     ILRPGITVVDLGAAPGGWSQVTSRVIGDKGCLIASDILEMDSIPDVTFIQGDFTDDVV
                     FTQILEAIGNKPVDLVISDMAPNMSGVRESDQPRAMYLCELALDLSTRVLRPGGDFLI
                     KIFQGEGFDLYHKQVRQLFDKVQMRKPLSSRDRSREQYMLARGFRGL"
BASE COUNT         1284 a         1840 c         1399 g         1059 t
ORIGIN      
        1 cgcaggtgat gcggtcaagc cggtgccaat agcccaaacc ggctcataag cgataactgc
       61 agcggcaaac acctcaacac ccaaagcatc agtcaccgca gataactgcg ctgcaaccac
      121 ttcaagcgtc cgccccgcct cacgctgagc aagcgtctca ccgacacaca gcaccggaag
      181 caagcccgaa gactgcgccg cagcaaactt gcgcgtaaca acttcatcac tctcacccag
      241 caacgaccta cgctcggagt gaccaaccaa caccagcgaa cagcgcgcgt catgcaactg
      301 acttgccgcc acctcaccag tcaaagcacc ttgctgcgcc tcggtcgcgc aatcttgcgc
      361 acctaccgca acagccttgc ctgccaaacc atcaaccacc tggccaagat gcaaacaaga
      421 cgggaaaacc gctacatcta catcagcagg caacacctgc tgattgagac cgttgatcag
      481 ctctacgacg ctggcgcggg taccgtgcat tttccagtta ccagcaacca ttgggcgacg
      541 catgccttac ctcgtcggtc aaagagggcg cagatgttac ccaacagcta cccgactggc
      601 aagccgaatc aagcacatac atccgcaaca actttagcca attcatcggc atagccgcgc
      661 acctggcctt catcatcacc ctcgaccata acgcgcacca atggctcggt gccggattta
      721 cgcagcaaca cccggccacg cccagccatt tgctcaatta cccgcgcgct agcctgctgc
      781 acagccggat gctccagcgg gtcaacaccg ccggcaaagc gtacattcac caacacctgc
      841 ggacacttct gcatacccag gcgcgcctca cccagggttt gctcacggcg cttaagcgcc
      901 atcaacacct gcagcgcggc gataattgcg tcgccggtcg tggtgtgctg gaaacacacc
      961 agatgccccg aattttcacc acccaactgc cagttacgcg acagcaactc ggctatcaca
     1021 taacggtcgc caaccttggc gcggacaaac ggaataccca gatcagccag ggctagctcc
     1081 agcccgaggt tactcattag cgtaccgacg acaccgccct gcagcttgcc gcgctccagc
     1141 agatcgcgag caatgataaa cagcaactca tcaccatcaa ccacggcgcc ggagtgatcg
     1201 accatcaaca cccgatccgc atcgccatca aaggcgatac ccagatcagc ctgctgcgcc
     1261 agcacttcag cctgcagcgc ctcgacgtgg gtcgaaccgc agtcgtcatt gatattcaag
     1321 ccattaggct gcgccgacag aacagttacc tgcgcgccca gctcgcggaa tacgcttggc
     1381 gccaccttgt acgccgcgcc atgggcgcaa tcgacaacaa tcttcagccc agcaaagtca
     1441 gtactggtcg gcacactgct cttacagaac tcgatatagc ggccggcggc atcattgatc
     1501 cgcgagacct tgcccagctg ctcggaatcc accacggtca tcggctgatc gagcaattct
     1561 tcgatcatca gctcgatttc atccggcagc ttagtgccct gccccgaaaa gaacttgatg
     1621 ccgttgtcgt gatgcggatt gtgcgaggca ctgatcacga tcccggcctc ggcatggaag
     1681 gtacgggtca ggtaggcgat tgccggtgtt ggcatcgggc cgagcagcat cacatccgca
     1741 ccggccgcag ataggcccgc ctccagcgca gactcgaaca tataaccgga gatgcgcgtg
     1801 tccttgccta tcagaatgcg gcacttgccc tgcttgcgaa acgccatccc ggccgcccag
     1861 ccgagcttga gcatgaattc cggggtaata gggaagtcac ctacacgccc acgaataccg
     1921 tcagtaccaa aatattttct acccatccca caattccttc taacctattc ggccgcttca
     1981 accgcggcga tcatccgtac aacatcaacc gtttgcgcca catcgtgcac tcgcagaatg
     2041 tgcgcaccct tgctcagcgc caacgcagcc aaggccaggc tgccatacag ccgctcgcct
     2101 acttcatggc ccagcacctt gccgatcata ctcttgcgcg acaccccgac cagcaaaggc
     2161 cgacctagcg catgtagcgc cgctaggtgt ttgaacagcg acagattgtg ctccagggtt
     2221 ttcgcaaaac cgaaacctgg atcaaggatg actcgctcag tcccaatccc agccgccgca
     2281 catgccgtta gccgctcaag cagaaactcg cgcacttccg ccacgacatc ggggtattga
     2341 gggttctgct gcatggtggt cggctcaccg cgcatatgca ttaggcacac tggcaaaccg
     2401 ctgtcagccg ccgcatccag cgcaccatca cgctgcagtg agcgcacgtc attgatcaag
     2461 cccgcgccca accgcgaagt ttcgcggatg accgcgggcg ttgaggtgtc caccgaaatg
     2521 atcacatcca gttcgcgcgc aattgcctcg acgaccggcg caacccgctc cagctcctca
     2581 accggagaga cagcgcgagc gccagggcgc gtcgactcac caccgacatc aatcagcgtt
     2641 gcacccgcct gtagcatcgc ctcggcatga cgcaaagccg catcacgctg accatagcgc
     2701 ccaccatcgg agaaggagtc aggggtgaca ttgaggatgc ccatcacttg cggacggctt
     2761 aaatcaagaa accggctgcc acaaggcagc cggtcaaagg ttggcgcaac agacatggaa
     2821 aaccttaaag ctcggcggct ggaccaccga ttggcttctc tggggactca acgacttcaa
     2881 ctggcgcagt cggcgtgcca ttgctgtcgt caccgccctg ccagcctttc ggctcacgcg
     2941 gcgcgaggcc attcatgatg tcttcgatct gctcggcatc aatggtttcg tacttcatca
     3001 gcgcttgcgc catggcgtcc agcttgtcgc ggttctcttc cagcaaacga cgagccgtgc
     3061 tgtagcagcc atcaataatg ctgcgtactt cctgatcaat caccttggcg gtgtcggcag
     3121 aaacattgct ctgattgctg cccatgctgc gcccaaggaa gacttcgccc tcttcttcgg
     3181 catacatcat cgggccaagc ttctccgaca aaccccactt ggtgaccatg tttttagcca
     3241 actgggtggc acgcatgatg tcgttggacg caccggtggt gacgccgtcg aagcccaggg
     3301 tcatttcttc agcgatacga ccaccgaaca acgagcagat ctgactggtc agtgcacgct
     3361 tgctcaggct gtaacgatct tcctcgggca ggaacatggt cacgcccaga gcacgaccgc
     3421 gcgggatgat cgacaccttg tagaccggat catgctcagg caccaggcgc ccgacaatcg
     3481 cgtgaccggc ttcgtggaat gcggtgttga gcttttcctt gtccgacatg accatggttt
     3541 tgcgctcggc gcccatcatg atcttgtctt tggccagctc gaactctttc atctcaacca
     3601 cacgcttacc ggcacgggca gcgaacagcg atgcctcgtt gaccagattg gccaggtcgg
     3661 caccggagaa gcccggcgta ccacgcgcaa tcacggccgg agcaacgtct tcacccattg
     3721 gcactttgcg catatgcact ttgagaatct gttcgcgacc acggatatcc ggcaagccca
     3781 ctacgacctg acggtcgaaa cggcccggac gcagcaaagc agggtcaagc acgtcaggac
     3841 ggttggtggc ggcaatgacg atgatgccgt cattcatttc gaaaccgtcc atctccacca
     3901 gcaactggtt gagagtctgc tcacgttcgt cgtgaccgcc acccatgccg gcgccacgat
     3961 ggcgacccac agcgtcgatc tcgtcgataa aaatgatgca tggcgcgtgc ttcttggctt
     4021 gctcgaacat gtcacgcaca cgggatgcac cgacaccaac gaacatttct acgaagtccg
     4081 aaccagaaat ggtgaagaac ggcactttgg cttcaccggc aaccgccttg gccagcaagg
     4141 ttttaccggt accaggcgag ccaaccatca gcactccgcg cgggatacgc ccgcccaggc
     4201 gctggaattt gcccggatca cgcaggaatt caaccagttc gctgacttct tccttggcct
     4261 cgtcgcaacc ggcaacgtca gcgaaggtgg tcttgacctg atcttccgag agcagacgcg
     4321 ccttggactt gccgaagctc ataggcccgc ccttaccgcc cgcgccacct tgcatctggc
     4381 gcataaagaa catgaacacg gcgatgatca ccagaatcgg gaagctggcg accagcagct
     4441 gagtccagat gctctgctgc tcaggctgct tgccgacgat ctcgacgttg ttgtccatca
     4501 ggtccttgat caggccattg tcctgaatcg ccggacgcac ggtctcgaac gacgaaccat
     4561 cggtacgggt accgctgatg atgaaaccat caaccgtcac tcgtttaacg ccgccatcct
     4621 gaacctgctg gataaactgc gaatagttga gtttgttcga ctcagtcggg ctggaaaaat
     4681 tgttcatcac cgtcaccaag acggcggcga tgatcagcca caggaccaga ttctttgcca
     4741 tgtcgttcaa ttagctaccc tctgaagcag gccccgtgtc gaagcgcgct tcgcatgacg
     4801 accagttatg taccgatcta acttactaca caacgcccac ccctggcaga cgccgtctgt
     4861 aaccctttat ttgttgtgag accgcaaagc cccaccacaa ttcgccgtaa agcctgcaac
     4921 tatcagaggc cgcgaaagcc ccgcgccaac atatattgct cgcgggagcg gtcacgcgag
     4981 gacagcggct tacgcatctg caccttgtcg aacagctgac gcacctgctt gtggtacaga
     5041 tcgaaacctt cgccctggaa gatcttgatc agaaaatcac cacccggacg caacactcgg
     5101 gtcgacaaat ccagcgccag ctcgcacagg tacatcgcgc gcggctgatc cgattctctt
     5161 accccactca tattgggggc catatcggaa atcacaaggt ctaccggctt attgccgata
     5221 gcttcgagga tctgcgtgaa caccacatcg tcggtaaagt caccctggat aaaggtgaca
     5281 tcagggatgc tgtccatctc cagaatatct gacgcgatca aacatccctt gtcaccaatc
     5341 acccgactgg tgacctgcga ccagccaccc ggcgctgcac ccagatcaac tacagtgatg
     5401 ccaggacgca ggatgcgatc tttctcctga atctccagca gcttgtagct ggcgcgcgag
     5461 cgatagccat ccttctgcgc cattttgacg taggggtcgt tgaaatgctc tttcagccaa
     5521 ccttgactgg tcttggaacg ggccacgcaa tacctcgaat gtgcaacgca tgaatatctg
     5581 gg
//
LOCUS       DASRUM010000065        21320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2043033, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000065 DASRUM010000000
VERSION     DASRUM010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 21320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21320
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043033"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /gene="der"
                     /locus_tag="VFF22_04590"
     CDS             <1..1336
                     /gene="der"
                     /locus_tag="VFF22_04590"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HZX16133.1"
                     /translation="WQGRTYIVIDTGGISGDEEGIDAKMAEQSLQAIEEADAVLFLVD
                     ARAGLSASDQMIGEHLRRRNKRSFLVINKVDNIDADLARAEFSSMGMGESLAIAGAHG
                     RGITQMLEAVLGSFPKDATEPEEGEEEEIVLEGQEAKRIPGPSEKDGIKLAIIGRPNV
                     GKSTLVNRMLGEERVIVYDQAGTTRDSIYIPFERDDEKYTLIDTAGVRRRGKIFEAVE
                     KFSVVKTLQAIQDSNVVVFVMDAREGVVDHDLNLLGFVLESGRALVIALNKWDGMEPS
                     ERDYVKVELQRRLFFVDFADIHFISALHGTGVGNLYKSVQASFKSAITRWPTNRLTTI
                     LEDAVSDHAPPMVNNRRIKLRYAHLGGANPPLIVIHGNQVEAVPKSYIRYLENTYRRV
                     LKLVGTPIRIEFKGGDNPYEGNKNTLTDRQVNKKRRLMSHHKKAEKKRKDKR"
     gene            1457..2605
                     /locus_tag="VFF22_04595"
     CDS             1457..2605
                     /locus_tag="VFF22_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZX16134.1"
                     /translation="MIISKLPNVGTTIFTRMSQLAVETGALNLSQGFPDFDGPQALRD
                     AVCRHIAAGHNQYAPMTGLPALREQVAAKIARSYGRVVSADAEVTIVPGATQGIFCAI
                     QTLIQPGDEVIVFDPSYDSYEPSVELAGGRCVHVPLALPDFAIDWQRLQDALSDKTRL
                     IILNSPHNPSGALISRAELDQLAALIRERDIYLISDEVYEHLVFDGIQHASVLAHEEL
                     YQRAFVVSSFGKTYHVTGWKTGYVVAPPALSAELRKVHQYVSFCGVTPLQWALADYMA
                     EHPEHVEELPAFYQAKRDLFCDLLAGSRFTFTRAAGTYFQLVDYSAIRNDLDDVAMAE
                     WLTREHGVAAIPVSVFYQTPPENLRLVRFCFAKRKETLRQAAEKLCAL"
     gene            2703..3485
                     /locus_tag="VFF22_04600"
     CDS             2703..3485
                     /locus_tag="VFF22_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZX16135.1"
                     /translation="MSNKTDLELALIQTTLAWQNPAANRAHFESLLTQARGADLVVLP
                     EMFSTGFSMDSAALAEPEDGPTSQWLREQAQALQAVVTGSLIIQAADGSYRNRLLWAR
                     PDGSVAHYDKRHLFRMAGEHKHYSAGEQQVLLEVKGWQVRPLICYDLRFPVWSRDPSG
                     TDLLLYTANWPAARRNHWNRLLPARAIENLCYVAAVNRIGEDGKGYAYSGDSQVLDFQ
                     GEALLEPGAAAGVFRITLKAAELAAYRERFPAHLDADSFSLA"
     gene            complement(3472..3843)
                     /locus_tag="VFF22_04605"
     CDS             complement(3472..3843)
                     /locus_tag="VFF22_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015635131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZX16136.1"
                     /translation="MRIGELEKRSGASRHTLRYYESLGLISALRRDNNYREYSPQTLG
                     DLGFIQQAQSMGFSLGEIGEILKARREQQLDCAQGAALVSRKLAEVERKLADLQQLRD
                     FLRSEKLRLEVSAAEQAARPG"
     gene            3920..4987
                     /locus_tag="VFF22_04610"
     CDS             3920..4987
                     /locus_tag="VFF22_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014756166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX16137.1"
                     /translation="MNKHCLVTGGSGFIGQHLLALLTRQGNSATLLMRQPQALPSLRA
                     QIEQLGGRGELLTAVAGDLGRIDLGLDTVARAQVPQAAVVFHLGAQFAWGLTAEQARR
                     VNVEGALAVAELAAQQGSRLLMVGGFMLANHEHLQRIGIDLQRPERTDWDKVYQRTGA
                     YEGSKLEAHFRVHARMAELQAPLTVVHPATVCGHSRSGHILPAQPLAGLIGNLAGGLL
                     GAIPGSTAHWLPLVSVDFLAELIVAAAFDEQQAGQEILAMDAATPNLQGMLQQLAQTL
                     GVSAPRRHMPIPLLRILLKIPGMPRLLHTEAESLDFIQTTRFDIAATNALAQRHQLQW
                     PDIGQALQATARYLANKPSAI"
     gene            complement(5009..6679)
                     /gene="leuA"
                     /locus_tag="VFF22_04615"
     CDS             complement(5009..6679)
                     /gene="leuA"
                     /locus_tag="VFF22_04615"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HZX16138.1"
                     /translation="MSMLKDPSSKYRPFTQIQIPDRTWPDKIIDKAPIWLSTDLRDGN
                     QSLIEPMDAEKKMRFFKCLLAVGLKEIEVGFPSASQTDFDFVRELIEGGHIPDGVTIQ
                     VLTQARDDLIERTFESLKGAKKAIVHYYNACAPSFRKIVFNQDKAGVKAIAVAAGQTI
                     KRLADAAPETQWGFEYSPEVFSSTEIDFAVEVCNAVIEVFQPTQANKLILNLPATIEC
                     ATPNNYADQIEWFGRHIDKRDSVLLSVHTHNDRGTGVAASELAVMAGADRVEGCLFGN
                     GERTGNVCLVTLALNLYTQGVDPQLDFSDIDTVRKVVEDCNQIPVHPRHPYVGDLVHT
                     AFSGSHQDAIRKGFAQQKADAVWEVPYLPIDPADIGRDYEAVIRVNSQSGKGGITFLL
                     EQEYGINLPRRMQIEFSQVVQKETDRLGLEMSAAQIYQLLEREYLQATAPYALKGHRL
                     QEENGTSAVDVEVINAGESQHWRGIGKGPLEALVAGLPVAVEIMDYSEHAIGAGTNAK
                     AAAYIELRVNGGRALHGVGIDENLTTASFRALFSALNRAIGQAAEQAA"
     gene            complement(6979..7521)
                     /locus_tag="VFF22_04620"
     CDS             complement(6979..7521)
                     /locus_tag="VFF22_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HZX16139.1"
                     /translation="MRIRAQDSCLLIIDIQERLFPAIAEHAELAANTLWLQQVAQRIG
                     VPVLLTEQYSKGLGPTIASLRAGHAPEAIVEKLHFSAVSEGELLQRSGGTRQQFIVCG
                     SEAHVCVLQTVLDLLEQGRAVFVVAEAVGSRKPSDKQLALQRMQQAGAVIVSREMVAF
                     EWLEQAGSELFRSISREFIR"
     gene            complement(7536..8363)
                     /locus_tag="VFF22_04625"
     CDS             complement(7536..8363)
                     /locus_tag="VFF22_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZX16140.1"
                     /translation="MRLFTLLTLFCLALPVHAEGFISRLLNKPVPGGVAVVDLGPAAS
                     APSVRYQNKPVLVIINEDQQRWIAIVGIPLSIKPGSQQISVNGSQTLSFQVDSKHYVE
                     QRITIKNQQQVNPNAKNLARIERELAEQTRAYQQFSARQPSNLLFDRPVNGPLSSPFG
                     LRRFFNGEERNPHSGLDFAANSGTPIKAPAAGKVILIGDYFFNGKTVFVDHGQGLISM
                     FCHLSAIGVKVGDEIPRGGVLGKVGATGRATGPHLHWNVSLNDARVDPAIFIGAFKP"
     gene            complement(8381..9760)
                     /gene="xseA"
                     /locus_tag="VFF22_04630"
     CDS             complement(8381..9760)
                     /gene="xseA"
                     /locus_tag="VFF22_04630"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZX16141.1"
                     /translation="MIKDPFQRLNLDREVLSVSQLNNRARLLLEDVFGGIWVEGEISN
                     LAKPASGHIYFTLKDSQAQVRCALFRQNAAKVRQALRDGLAVKVRGKVSLFEGRGDYQ
                     LILDAVEPAGDGALRLAFEALKEKLSAEGLFAAEAKVALPAHPKRIGIISSPTGAVIR
                     DIISVFRRRAPQVELTLIPTAVQGREATAQIVRALGLADRRGFDALILARGGGSLEDL
                     WCFNEEAVARAITACVTPIVSAVGHETDVSIADFVADVRAPTPSAAAELLAPDSSELV
                     QRLHNLKRRLNLRMQHRLERERMRLDGLQRRLRHPGERLRQHAQRVDDLDMRLTRAFA
                     RQLNSRRERLNHLQTRLLSLHPERNLKLLDQRLQALAERLQRSMREGLKARRLQLHSQ
                     VQTLHAVSPLATLGRGYSILLDERGRAIRNAADTRPGQRLKARLGNGELDVRVEDNHV
                     QPVTLSLLD"
     gene            9866..10420
                     /locus_tag="VFF22_04635"
     CDS             9866..10420
                     /locus_tag="VFF22_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATPase"
                     /protein_id="HZX16142.1"
                     /translation="MSEQQAIIVPQISSFPGHEARARLIVRWLVKQKIIEEQLSTCGR
                     TFKHMGYGIAEGARRVVERPELLPFGQAVHGLEIIYKRCIYTPTTGFLEEAGCPECRR
                     EVGGRLFDSLEEWMPGHTDNFRCPLCGHEDDINGFLFLQPCGFSNLGFIFNNWADAGF
                     KESFLDEFAACLGYPVSLVRVDLS"
     gene            complement(10452..12503)
                     /locus_tag="VFF22_04640"
     CDS             complement(10452..12503)
                     /locus_tag="VFF22_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005734727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HZX16143.1"
                     /translation="MPAASSLAAATTSPVELPSQSVTATREDGSNYSASTASTASKSA
                     VSIMQEAQAVQVVTEQVIEDYQVQSLDDAMKFVSGMTQSNTLAGTQDGFVKRGFGSNA
                     DGSILRDGIRSSLSRNFSATTERVEVLKGPASLLYGALEPGGLINVISKQPEYQRRSQ
                     ISGETSSEGGGNLMLDTTGPLGDSGFAYRLIAERQHEDYWRNFGADNHTLVAPSLSWT
                     GEDLRISLAYEHKEYSTPVDRGTVIINGKPANTEYNARFNESWAKAEGTDELFTAKAE
                     YQLNPNWLSRLTYGWNNERYDYAEARPNALNTTTGALSRRADGSEHDNQTQYLAWDWI
                     GNASVFGQQHDLLLGMDTERVDNFRGDTYRGPAVGGFNIYRPVYGNLAEPSLISAAQS
                     DRSDELHSRSLYVKDNWHLNDQWILVAGGRYQHFEQLVEQGRGSAYEKTTDRQDNNFL
                     PFAGLVYQLNQQVSLYGNYSRSFVPNASDADTGQAFDPEQGRSYELGIKLDLGNALTA
                     SAAVFDIEKENVVVSNNGTSQAAGKVGSQGVEIDISGRIAERWELLGTYAYTDTEILD
                     DPANQGNELVNAAQNIASLYLTHHLALPSDAGQWRVGGGARYVGERAGDNANSFWLDS
                     YTVADAFISWESQLLGDKTRLQLNARNLFNQEYYPSSGGNLRVAVGEPRELRLSASVD
                     F"
     gene            12728..14197
                     /gene="guaB"
                     /locus_tag="VFF22_04645"
     CDS             12728..14197
                     /gene="guaB"
                     /locus_tag="VFF22_04645"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZX16144.1"
                     /translation="MLRISQEALTFDDVLLIPGYSEVLPKDVSLKTRLTRGIELNIPL
                     LSAAMDTVTEARLAIAMAQEGGIGIIHKNMTVEQQAAEVRKVKRYEAGVVKDPITIEA
                     DATVRDLFELTRMHNISGVPVLSDGDLVGIVTSRDVRFENRLDATVREVMTPKERLVT
                     VPEGADKDLVRELLHKHRIEKVLIVDGAFHLKGMMTVKDIEKAKAYPLASKDDQGRLR
                     VGAAVGTGADTADRVAALANAGVDVIIVDTAHGHSKGVIDRVRWVKQNFPDVQVIGGN
                     IATGEAAKALAEAGADAVKVGIGPGSICTTRIVAGVGVPQISAVANVAAALAGTGVPL
                     IADGGIRFSGDLSKAIVAGASCVMIGSMLAGTEEAPGEVELFQGRSYKAYRGMGSLGA
                     MAQAQGSSDRYFQDSSAGAEKLVPEGIEGRVPYKGAMAAIVHQLMGGLRASMGYTGCA
                     TIEEMRSKPEFVRITGAGMAESHVHDVQITKEAPNYRVG"
     gene            14290..15870
                     /gene="guaA"
                     /locus_tag="VFF22_04650"
     CDS             14290..15870
                     /gene="guaA"
                     /locus_tag="VFF22_04650"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZX16145.1"
                     /translation="MAHQDIHAHRILILDFGSQYTQLIARRVREIGVYCELHPWDMSD
                     DDIRAFAPRGIILAGGPESVHAADSPRAPKAVFDLGVPVFGICYGMQTMAEQLGGKVE
                     GSDLREFGYARVDIVGKARLLNGIEDHIDDDGVFGLDVWMSHGDKVTAMPAGFHILAS
                     TPSCPIAAIADDSRGYYGVQFHPEVTHTKQGGRILSRFILEICGCEALWTASNIVEDA
                     IANVRAQVGSANVLLGLSGGVDSSVVAALLHKAIGDQLTCVFVDNGLLRLHEGEQVMA
                     MFAENMGVKVIRANAEEQFLGNLAGEADPEKKRKIIGRTFIDVFDAESCKLDNIKFLA
                     QGTIYPDVIESAGAKSGKAHVIKSHHNVGGLPEEMNLKLVEPLRELFKDEVRRLGLEL
                     GLPYDMVYRHPFHGPGLGVRILGEVKKEYADLLRRADHIFIEELRKADWYHKVSQAFV
                     VFQPVKSVGVVGDGRRYAWVVALRAVETIDFMTARWAHLPYELLETVSGRIINEIEGI
                     SRVTYDVSSKPPATIEWE"
     gene            complement(16046..17251)
                     /locus_tag="VFF22_04655"
     CDS             complement(16046..17251)
                     /locus_tag="VFF22_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16146.1"
                     /translation="MRIRYTVPFKRKIIIDDHWQIPIQGGKLRVIEEAGYAKALEITF
                     EKQPLEYAPRLKPPSDSEPLTTITGRDHRMIFVKNLLDDAATLLECIYDIELVTDEID
                     AKYEGETPEEETKIQVKGISKGRHDSALPLSFDMLTRAIMAADKHQVSRFEAPLVRSA
                     RKALDAQEFINSFRYSFLLIESLYGEGKFKKEGLQTSLKNNQEFRDILDFVIKERTSA
                     RSDHTSDTARLLVTNPTIDDVIVHLVEKRGFYFHGNVKRKDAWRPDEQGVAESLALLA
                     VGIATRISLKAADSIFNPELGQRHYDDAMRVGAEIMFEIKLKFREPEEKFDREQQINI
                     SMPGTKVTPRSAFAVAQHFLQLFQDNQPVSALREAECTVQGTGEKVFNLTFHATEDER
                     EKTTSEKSD"
     gene            complement(17372..17666)
                     /locus_tag="VFF22_04660"
                     /pseudo
     CDS             complement(17372..17666)
                     /locus_tag="VFF22_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011898609.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            17812..19182
                     /locus_tag="VFF22_04665"
     CDS             17812..19182
                     /locus_tag="VFF22_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HZX16147.1"
                     /translation="MSFTRRQMLVGLAGLGVVGLGTGGVRYWLGRPENVATHDYELIA
                     APLDIELVPGHTTPAWAYGGQAPGVELRCRQGERLRVRFINRLDVPTTIHWHGIRLPL
                     EMDGVPYVSQAPVLPGEYFDYNFITPDAGSFWYHPHVASGEQLGRGLVGALIVEEREP
                     TGFRHDHTLNLKSWHIDEQGAFTAFSVPREAARGGTPGRLSTVNGKHAPTLDLPAGQV
                     VRLRLINLDNTLTYRLNLPGADARIYALDGNPVEPRPLGKAYWLGPGMRIELGLKVPA
                     AGTELSLRNGPLRLATLRAQPGVEVAGEWPSALPANPVAEPDLAAAETLRFNFEWAGA
                     VSVGLAQGAAHSFWQINGQAWDINDKTCAERPIARLQLGKSYIFELRNLSQYQHPIHL
                     HGMTFKVLASNRKAVIPYFTDTYLLGKNESARVALVADNPGVWMFHCHVIDHMETGLM
                     ASIEVV"
     gene            19238..19720
                     /gene="tadA"
                     /locus_tag="VFF22_04670"
     CDS             19238..19720
                     /gene="tadA"
                     /locus_tag="VFF22_04670"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HZX16148.1"
                     /translation="MKKVQIIDRSRDSFFMREALALAAEGAALGEVPVGAVLVQDGAI
                     IGRGFNCPISSHDPSAHAEMVAIRAAARAVDNYRLPGSTLYVTLEPCSMCAGLIVHAR
                     VQRVVYGATEPKAGVALSRGQFFSQEFLNHRVLIEGGVLAEECGAVLSAFFKARREQR
                     "
     gene            complement(19862..>21320)
                     /gene="mltF"
                     /locus_tag="VFF22_04675"
     CDS             complement(19862..>21320)
                     /gene="mltF"
                     /locus_tag="VFF22_04675"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HZX16149.1"
                     /translation="FAQSAFRVRCACWLSVIGILLVLGGCAEEPSTLERVQAEGVLRV
                     ITRNSPATYFQDRNGETGFEYELVKRFADDLGLELKIETADNLDELFASLNKPNGPVL
                     AAAGLVESDGRQQHARFSLPYLEVTPQVIYRNGQQRPTRPDDLLGKRILVLKGSSHAE
                     QLAALKLQLPELVYEESAAVEVVDLLRMVDEGQIDLTLVDSNELAMNQVYFSNVRVAF
                     DLGDAQNLAWATAPGEDRSLLDEMNAFIERAHQNGSLQRLKDRYYGHVDVLGYVGAYT
                     FAKHLQQRLPRYEKHFREAAQANQVDWRLLAAMGYQESLWQPTATSKTGVRGLMMLTL
                     RTAQAMGVSDRLNPKQSIEGGAKYIVYVKNQLPESIQEPDRTWFALASYNVGGGHLED
                     ARKLTEAEGLDPNKWLDVQKILPRLSQKQWYSKTRYGYARGGEPVHFVRNIRRYYDIL
                     TWVTQPQLEGSQIAESNQHVPGIDKRQPNEQTPPL"
BASE COUNT         3855 a         6111 c         6744 g         4610 t
ORIGIN      
        1 gtggcagggg cgaacctata tcgtcatcga caccggtggt atttccggtg acgaggaagg
       61 cattgacgcg aagatggccg agcagtcgct gcaggccatc gaagaggccg atgccgtact
      121 gtttctggtg gatgcccgcg ccgggttgtc tgcttccgat cagatgatcg gcgagcacct
      181 gcgtcggcgt aacaagcgca gcttcctggt catcaacaag gtcgacaaca tcgacgccga
      241 cctggcccgc gctgagttct ccagcatggg catgggcgag tccctggcga ttgccggtgc
      301 tcacggtcgc ggcatcaccc agatgctcga agccgtgctg ggctccttcc ccaaggatgc
      361 caccgagccg gaagagggcg aggaagagga aatcgttctc gaaggtcagg aggccaagcg
      421 cattcccggc ccgagcgaga aggatggcat caaactggcg atcatcggcc ggcctaacgt
      481 tggcaagtcg accctggtca accgtatgct cggtgaagag cgggttatcg tttatgacca
      541 ggccggcacc acccgcgaca gtatctacat cccgttcgag cgcgatgacg agaagtacac
      601 cctgatcgac accgctggcg tacgtcgtcg cggcaagatt ttcgaagcgg tggaaaagtt
      661 ctcggttgtg aaaaccctgc aggcgatcca ggactccaac gtcgtggtat tcgtcatgga
      721 cgcgcgcgaa ggtgtggtcg atcacgacct caacctgctt ggcttcgtgc tggaaagcgg
      781 ccgcgccctg gtaatcgccc tgaacaaatg ggacggcatg gagccgagcg agcgcgatta
      841 cgtgaaggtc gagctgcaac gccggctgtt cttcgtcgac tttgccgata tccactttat
      901 ctcggcgctg cacggcaccg gcgtgggcaa cctgtacaag tcggtgcagg cctcgttcaa
      961 gtcggcgatc acccgctggc cgaccaaccg tctgaccacg attcttgaag acgccgtcag
     1021 cgatcacgcg ccgcccatgg tcaacaatcg ccgcatcaag ttgcgctatg cccacctggg
     1081 tggtgccaac ccgccgctga tcgtgatcca cggtaaccag gtcgaggcgg tgcccaagtc
     1141 ctatatccgc tacctggaaa acacctaccg tcgcgtgctt aagctggtcg gtacgccgat
     1201 ccgtatcgag ttcaagggtg gcgataaccc ctatgagggc aacaagaaca ccctcaccga
     1261 ccgtcaggtg aacaagaagc gccgcctgat gagccaccac aaaaaagccg agaagaagcg
     1321 caaagacaag cgctaagctt ggatttaaac gctctataaa ggcaccttcg ggtgcctttt
     1381 gcgtctctgg gggatcggct atcctgcggc atcactagcg ctcaagctcg gcgcttggcc
     1441 caggaatatt gcctcgatga ttatcagcaa actgcctaat gtcggcacca ccatctttac
     1501 ccgtatgtcg cagctcgcgg tggaaaccgg cgcgctcaat ctgtcccagg gcttcccgga
     1561 tttcgatggc ccgcaggcct tgcgcgatgc ggtctgccgg catattgccg ccgggcataa
     1621 tcagtacgcg ccgatgaccg gcttaccagc gctgcgcgag caggtggcgg cgaagattgc
     1681 ccgcagttat gggcgtgtgg tcagtgccga tgctgaagtc accatagttc ctggcgctac
     1741 ccaaggcatc ttctgcgcta tccaaacgct gattcagccg ggcgatgaag tgatcgtctt
     1801 cgatcccagc tacgacagct acgagccctc ggtcgagctg gcaggcgggc gctgcgtgca
     1861 tgtgccactg gccttgccgg atttcgccat cgactggcag cgcctgcagg atgcgctgag
     1921 tgacaagact cgcctgatta tcctcaacag cccgcacaac cccagcggtg cgctgatctc
     1981 ccgcgccgag ttggatcaac tggcggcgct gattcgcgag cgcgatatct acctgatcag
     2041 cgacgaggtt tacgagcatc tggtgttcga tggtattcag cacgccagcg tgctggccca
     2101 tgaggagttg tatcagcgcg cctttgtggt cagctcgttc ggcaagacct accacgtcac
     2161 tggctggaaa accggctatg tggtggcccc gccggccttg agcgccgagc tgcgcaaggt
     2221 gcatcagtac gtcagcttct gcggcgtgac gccgctgcaa tgggccctgg ctgactatat
     2281 ggccgagcat cccgagcacg tcgaagagtt gccggcgttc taccaggcca agcgcgatct
     2341 gttctgcgac ctactggctg gctcgcgctt taccttcacc cgcgccgccg gcacctattt
     2401 ccagctggtc gattactcgg ccattcgcaa tgatctggac gatgtggcca tggccgaatg
     2461 gctgacccgc gaacatggcg tagcggcgat tccggtatcg gtgttctacc agacaccacc
     2521 tgagaacttg cgcctggtgc gcttctgctt cgccaagcgc aaagaaaccc tgcgtcaggc
     2581 ggcggaaaaa ttatgcgcct tgtaggtgtg tagggtggac atggcgaagc cttgtccacc
     2641 ttggtattgc aatggcggac aagccttcgg catgtccgcc ctacggctat tgaggccttg
     2701 agatgagtaa caaaaccgat cttgaactgg cactgattca gaccaccctg gcctggcaga
     2761 accccgcggc caatcgcgcg catttcgagt cgctgctgac gcaggcgcgt ggtgcggatc
     2821 tggtcgtact gccggagatg ttcagcaccg gcttttccat ggactccgcc gccctggccg
     2881 agccagaaga tggtccaacc agccagtggc tacgcgagca agcccaggcg ctgcaggctg
     2941 tcgtcaccgg cagcctgatt atccaggctg ccgatggctc ctaccgcaac cgcctgctct
     3001 gggcgcgccc ggatggctct gtagcgcact acgacaagcg ccatctgttc cgcatggccg
     3061 gtgagcacaa gcactacagc gcaggtgaac agcaggtgct gctggaggtg aagggctggc
     3121 aggtgcgccc gctgatctgc tacgacttgc gcttcccggt ctggagtcgc gacccaagcg
     3181 gtactgacct gctgctgtat accgccaact ggccggcggc gcgtcgcaat cactggaacc
     3241 gcctgttgcc ggcgcgggcc atcgaaaacc tctgttatgt cgcggcggtc aaccgcatcg
     3301 gcgaggacgg caagggctat gcctacagcg gtgacagcca ggtgctggat tttcagggcg
     3361 aagcgttgct tgagcccggg gcggcggctg gcgtgtttcg cataaccctc aaggcggcgg
     3421 agctggctgc ttatcgtgag cgttttccag cccatctgga tgcagacagt tttagcctgg
     3481 cctagccgcc tgttcggcgg cgctgacctc cagacgcagc ttttcgctgc gcagaaagtc
     3541 gcgcagctgc tgcaggtcgg caagtttgcg ctccacctcg gccagcttgc ggctgaccag
     3601 cgccgcgccc tgggcgcagt cgagctgttg ttcacgtctg gccttgagga tttcgccgat
     3661 ctcgcccaag gaaaagccca tgctctgggc ttgctggata aaacccagat cgcccaaggt
     3721 ctgcgggctg tattcgcggt agttgttgtc acgccgcagg gcgctgatca gccctaggct
     3781 ttcgtaataa cgcagggtgt ggcggctagc gccgctgcgt ttttccagtt ctcctatgcg
     3841 catgaaaatc tcgcttgact atagagtgtg ctcgatagtt tagcctgctc gggtcactaa
     3901 tggttcgggt ggattgatca tgaacaaaca ctgtttggta accggcggca gcggctttat
     3961 cggtcagcat cttctggcgc tactgactcg tcagggcaat tcggcgacgc tgctgatgcg
     4021 ccagccacaa gccttgccgt ccttgcgtgc gcagattgag caattaggtg gccgcggtga
     4081 gctgctgacg gctgtggccg gtgacttggg gcgcatcgac ctgggcctgg ataccgtggc
     4141 gcgggcgcag gtgccccagg cggctgtggt gtttcacctg ggtgcgcagt tcgcctgggg
     4201 gctgactgcc gagcaggcgc gccgggtcaa tgtcgaaggc gcattggcgg tggccgagct
     4261 ggcggcgcag cagggcagcc gtttgctgat ggtgggcggc tttatgttgg ccaatcacga
     4321 gcatctgcag cgcatcggca tcgatctgca gcggcccgag cgcaccgact gggacaaggt
     4381 ttatcaacgt actggcgcct atgagggcag caagctagag gcgcattttc gcgtgcatgc
     4441 gcgtatggct gagctgcagg cgccactaac ggtggtgcat ccggccaccg tatgcggtca
     4501 tagccgcagt gggcatattt tgccggcgca gccgttggct gggctgatcg gcaatctggc
     4561 gggcggtttg ctcggggcaa taccaggctc gacagcgcat tggctgccgc tggtcagtgt
     4621 ggattttctc gccgagctga tagtggccgc tgcatttgat gagcagcagg ccgggcagga
     4681 gattctcgcc atggatgctg ccacgccgaa cctgcaggga atgctgcagc aattggcgca
     4741 gacgctgggc gttagtgcgc cgcgtcggca tatgccgatt cccttgctgc gtatcttgct
     4801 gaagattcca ggtatgccgc gtttgttgca taccgaggcc gagtcgctgg actttatcca
     4861 gaccacccgt ttcgatatag ctgcgaccaa tgccctggcg cagcgccatc aactgcagtg
     4921 gccggatatc ggccaggcgt tgcaggcgac agcgcgctat ctggcgaata agccgtcagc
     4981 catatagcgc tgacggcttc tgcagtgatc aggcggcttg ctcggcggcc tggccaatgg
     5041 cccgattcag tgcgctaaac agcgcgcgga agctcgcggt agtcaggttt tcatcgatgc
     5101 cgacgccatg cagcgcgcga ccgccgttaa cgcgcagctc aatataggcc gccgccttgg
     5161 cgttggtgcc ggcgccgatg gcgtgttcgc tgtagtccat gatctccacg gccaccggta
     5221 aaccggcgac cagggcctcc agcgggcctt tgccgatacc gcgccagtgt tggctttcgc
     5281 ctgcattgat gacttcgaca tcgaccgcgc tggtgccgtt ctcttcctgc aggcgatggc
     5341 ctttcagcgc atagggtgca gtggcttgca ggtactcgcg ctccagcagc tggtagatct
     5401 gcgcggcgct catttccagg ccgagacggt cggtttcctt ctgcaccacc tggctgaact
     5461 cgatctgcat gcgtcgcggc aggttgatgc cgtattcctg ttcgagcagg aaggtgatgc
     5521 cacccttgcc ggactggctg ttgacgcgga ttaccgcctc gtaatcgcga ccaatatcgg
     5581 ccgggtcgat cggcagatac ggcacttccc acacggcgtc agccttctgc tgggcgaagc
     5641 ctttgcggat cgcatcctgg tgtgagccgg agaaagcggt atgcaccagg tcgccgacgt
     5701 acggatggcg cgggtgtacc gggatctgat tgcagtcctc gaccaccttg cgcacggtgt
     5761 cgatatcgga gaagtccagt tgtgggtcga cgccctgggt gtagaggttc agcgccaggg
     5821 tcaccaggca gacgttgccg gtgcgctcgc cattgccgaa caggcagcct tcgacgcgat
     5881 ccgcgccagc catgacggcc aattctgagg cggcgacacc ggtgccacgg tcgttatggg
     5941 tgtgcacgct gagcagcacg ctgtcgcgct tgtcgatatg ccggccgaac cactcgatct
     6001 ggtcggcata gttgttcggc gtggcgcact cgatggtcgc cggcaggttg aggatcagct
     6061 tgttcgcctg ggtcggctgg aacacctcga tcactgcgtt gcacacctca acggcgaagt
     6121 ctatttcggt ggagctgaac acttccggcg aatactcgaa gccccactgg gtttccggcg
     6181 cggcatcggc caggcgtttg atggtctggc cggcggccac ggcgatagct ttgacgccgg
     6241 ccttgtcctg gttgaagacg atcttgcgaa agctcggtgc gcaggcgttg tagtagtgaa
     6301 cgatggcctt cttcgcgcct ttcagcgact cgaaggtgcg ttcgatcagg tcgtcacggg
     6361 cctgggtcag tacctgaatg gtcacgccat cggggatatg gccgccttcg atcagctcgc
     6421 ggacgaagtc gaaatcggtc tgcgaggcgg acgggaagcc gacttcgatt tctttcagac
     6481 ccacggccag caggcacttg aagaagcgca tcttcttctc ggcatccatc ggctcgatca
     6541 gcgactggtt gccatcgcgc aggtcggtgg acagccagat gggtgccttg tcgatgatct
     6601 tgtccggcca ggtacggtct ggaatctgga tctgggtaaa tgggcggtat ttgctggacg
     6661 ggtctttaag catgctcatg gggcaatcct tttagcgcgg tggcaatcgg cgaaccgggc
     6721 ggaatttgaa aaaataaggc agggcgtggg cgtaagaatt agccacgcag tcgctggctg
     6781 accaagcaga ggttggcgtg ttggcgcagc agaataagag tgtgattagt gtgcatggcg
     6841 tcaaccctaa ccagcgaggg taaagcgtgc aagcctgcct gaaaaattgg tgataaatcg
     6901 cgccatgtgt agatgattgc gattttatag cgttatggtg ttctggtttg gccttggatt
     6961 gcgcggccta tagcgctgct agcggataaa ttcccgactg atgctgcgaa acaattcgct
     7021 gccggcctgc tccagccatt cgaaggcgac catttcccgc gagacgatca ctgcgccggc
     7081 ttgctgcatg cgctgcaacg ccagttgctt gtcggatggt ttgcgcgagc caacggcctc
     7141 ggccacgaca aatacggcgc ggccttgctc cagcaggtcg agcacggtct gcagcacgca
     7201 gacatgggct tcgctgccgc acacaataaa ctgttgccgt gtaccgccag agcgttgcaa
     7261 aagctcgccc tcgctgactg cggagaagtg cagtttttct acaattgcct cgggcgcatg
     7321 cccggcgcgt agcgaggcaa tagtcggccc cagacccttg ctgtattgct cggtgagcaa
     7381 cacgggtacg ccgatacgct gcgccacctg ctgcaaccac agggtattgg cggccagctc
     7441 ggcatgctcg gcgatggcag ggaacaagcg ctcctgaata tcgataatca gcaggcagga
     7501 gtcttgagca cggatacgca tggtggtgtt cctgttcagg gcttgaaggc accgataaag
     7561 attgccgggt cgacccgggc gtcgttcagg ctgacgttcc agtgcaggtg cggcccggtg
     7621 gcgcggccgg tggcaccgac tttgccgagc acgccgccac ggggaatttc atcgccgacc
     7681 ttcacgccga tggccgagag gtggcagaac atactgatca ggccctggcc gtggtcgaca
     7741 aataccgtct tgccgttaaa gaagtagtcg ccaatcagga tcaccttgcc ggcggccgga
     7801 gccttgatcg gcgtgccgct attggccgcg aagtccagcc ccgagtgcgg gttacgttcc
     7861 tcgccgttga agaaacggcg caggccgaag gggctggata gcggcccgtt caccggcctg
     7921 tcgaacagca gattgctcgg ctggcgcgcg ctgaactgtt ggtaggcgcg ggtctgctcg
     7981 gccagttcgc gctcaatgcg tgcaaggttc ttggcgttcg ggttgacctg ctgttgattc
     8041 ttgatggtga tgcgttgctc gacgtaatgc ttactgtcca cctgaaagct cagcgtctgg
     8101 ctgccgttga cgctgatctg ctggctgccc ggcttgatgc ttagcgggat gccaacgatg
     8161 gcgatccagc gttgctgatc ttcattaata ataaccagca ccggtttgtt ctgatagcgc
     8221 acgctgggtg cgctggcggc tggccccagg tccaccacgg ctacgccgcc gggtactggc
     8281 ttattgagca gtctgctgat aaagccttcg gcgtggacgg gcagggcaag gcagaacagg
     8341 gtaagcaggg tgaataaacg catgggggac ctgtgtggaa tcaatcgagc agcgagaggg
     8401 tgaccggctg tacatggttg tcttcgaccc gcacatccag ctcgccgttg cctaggcgcg
     8461 ccttcaggcg ctggccggga cgggtgtcgg cggcgttgcg gatagcacgg ccgcgctcat
     8521 ccagcaggat gctgtagccg cggccgaggg tcgccagcgg gctgaccgcg tgtagcgtct
     8581 gcacctggct atgcaactgc agacggcgcg ccttgaggcc ttcgcgcatg ctgcgttgca
     8641 ggcgttcggc cagtgcctgc aggcgctgat ccagcagctt gaggttgcgt tccgggtgca
     8701 ggcttagcag gcgggtttgc agatgattca agcgttcgcg acgactgttc agctggcgcg
     8761 cgaaagcgcg ggtcaggcgc atatccagat catccacgcg ctgggcgtgc tggcgcaggc
     8821 gctcgccagg atggcgcaag cgccgctgca aaccgtccag gcgcatgcgt tcgcgctcca
     8881 ggcggtgctg catacgcagg ttcaggcggc gcttgagatt gtgcaggcgc tgcaccagtt
     8941 cgctgctatc aggtgccaat agctcggcgg cggcagaggg cgttggtgca cgcacgtcgg
     9001 cgacgaagtc ggcgatggag acatcagtct catggcccac agcgctgacg atgggtgtga
     9061 cacaggcggt aatggcgcgg gctacggctt cttcgttaaa gcaccagagg tcctccaagg
     9121 aaccgccgcc acgggccagg atgagcgcat cgaagccgcg gcggtcggcc aggccgaggg
     9181 cgcggacgat ctgtgcggtg gcctcgcggc cttgcacggc ggtggggata agggtcagtt
     9241 cgacctgcgg ggcgcggcgg cggaatacgc tgataatgtc gcggatcact gcgccagtgg
     9301 gcgagctgat aatgccgatc cgtttcggat gggccggcag ggcaacttta gcctcggccg
     9361 cgaacagccc ttcagcgctg agtttttcct tcagcgcttc gaaggccagg cgcagcgcac
     9421 catcgccggc aggttccacg gcatcgagaa tcagctggta atcgccgcgc ccttcaaaca
     9481 gcgagacctt gccgcgcacc ttgaccgcca ggccgtcccg cagcgcctgg cgcaccttgg
     9541 cggcgttctg ccggaacagc gcacagcgca cctgggcctg gctatcttta agggtgaaat
     9601 agatatgccc ggacgccggc ttggccaggt tggagatttc cccctcgacc cagatgccgc
     9661 cgaatacatc ttccagcagc aggcgtgcgc gattgttcag ttgactgacg ctgagcacct
     9721 cgcggtcgag gttcaggcgt tggaaggggt ctttgatcat ggcccgcata gtaaaggctt
     9781 tgccctgcct gccatagggg attggccttg accgccgctg taagcccttt aagctgcgcg
     9841 cctttagcgc aaaccgagtc ccgttatgag tgagcagcag gccattatcg tgccgcagat
     9901 ttccagcttt cccggccatg aggcgcgggc gcggttgatc gtgcgctggc tggtcaagca
     9961 gaaaatcatc gaagagcagc tgagcacctg cgggcgtacg ttcaaacata tgggctatgg
    10021 catagccgag ggtgcgcgga gggtggtcga gcgtcccgag ctgttgccgt tcgggcaggc
    10081 ggtgcatggt ctggagatta tctataaacg ctgcatctac accccgacga cgggctttct
    10141 cgaagaggcc ggttgccctg agtgcaggcg ggaagtaggg ggaaggttgt tcgatagcct
    10201 ggaggagtgg atgccggggc ataccgataa cttccgctgt ccgctgtgcg gccatgaaga
    10261 cgatatcaac ggctttctgt ttctgcagcc ctgcggcttt tccaacctgg gatttatctt
    10321 caacaactgg gcggatgccg gcttcaagga gagctttctc gatgagtttg ctgcgtgctt
    10381 gggctatccg gtttccctgg tgcgggtcga tctttcttaa gtaggctcat ccctgagccc
    10441 tgctgaaaag gtcaaaagtc gacgctggcc gataggcgta gttcgcgcgg ttcaccgacg
    10501 gcgacgcgca ggttgccgcc gctggagggg tagtactcct ggttgaacag gttgcgcgcg
    10561 ttgagctgca ggcgggtctt gtcgcccagt agttggcttt cccaactgat aaacgcatcg
    10621 gccacggtgt agctgtccag ccagaagctg ttggcgttat cgccggcgcg ttcaccgacg
    10681 taacgcgcgc cgccgcccac tcgccactgt ccggcatcgc tgggcagcgc cagatggtgg
    10741 gtcaggtaca ggctggcgat attctgcgcg gcattgacca gttcattacc ttggttggcc
    10801 gggtcgtcga ggatttccgt atcggtgtag gcgtaggtgc cgagcagctc ccagcgttcg
    10861 gcaattcgcc cgctaatatc gatttccaca ccctgcgaac cgaccttgcc ggcggcttgc
    10921 gaggtgccat tgttgctgac cacgacgttc tctttctcga tatcgaacac tgcggcgctg
    10981 gccgtaaggg cgttgcccag gtcgagcttg atgcccagct catagctgcg accctgttcc
    11041 gggtcgaacg cctggccggt gtcggcatca gaggcgttgg gcacgaatga gcggctgtag
    11101 ttgccgtaca gcgagacttg ctgattgagc tgatagacca ggccggcaaa cggcaaaaag
    11161 ttgttgtcct ggcggtcggt ggttttctcg taggcgctgc cacggccctg ttcgaccagt
    11221 tgttcgaagt gttgataacg accaccggcc accagtatcc attggtcgtt aaggtgccag
    11281 ttgtccttca catacagcga gcgtgagtgc agctcatcgc ttctgtcgct ctgcgcggcg
    11341 ctgatcagcg agggttcggc aaggttgccg tagaccgggc ggtagatatt gaagccgccg
    11401 acggccgggc cgcggtaggt atcaccgcgg aagttgtcga cgcgctcggt gtccatgccc
    11461 agcagcagat catgttgttg gccgaacacg ctggcgttgc cgatccagtc ccaggccagg
    11521 tactgggtct ggttgtcgtg ttcgctgccg tcggcacggc ggctgagtgc gccggtggtg
    11581 gtgttaagcg cgttgggccg cgcttcggca tagtcgtagc gctcgttgtt ccagccgtag
    11641 gtcaggcggc tcagccagtt cgggttgagt tggtactcgg ccttggcggt aaacagctca
    11701 tcggtgcctt cggcttttgc ccagctttcg ttaaagcgtg cgttgtactc agtgttcgcc
    11761 ggtttgccgt tgatgatcac cgtgccgcga tccaccgggg tcgagtattc cttgtgctcg
    11821 taagccaggc tgatacgcag gtcttcaccg gtccagctga gggacggggc taccagggtg
    11881 tggttgtccg cgccgaagtt gcgccagtag tcctcgtgct gccgttcggc gatcaggcga
    11941 taggcgaagc cgctgtcgcc caacgggccg gtggtgtcga gcatgaggtt gccgccgccc
    12001 tcgctgctgg tctcaccgct gatctgcgag cgccgctggt attcgggctg cttgctgatc
    12061 acgttgatca ggccgcccgg ctccagtgcg ccgtagagca gggaggccgg gcctttgagt
    12121 acttccactc gctcagtggt ggcgctgaag ttgcgcgaca ggctggagcg gatgccgtca
    12181 cgcagaattg agccgtcggc gttgctgccg aaaccccgtt tgacgaagcc gtcctgggtg
    12241 ccggccaagg tgttgctctg ggtcatgccg ctgacgaatt tcattgcatc gtccagcgat
    12301 tgcacttggt agtcctcaat cacctgctcg gtgaccactt gcaccgcctg ggcctcctgc
    12361 ataatcgaca ccgcgctttt gcttgcagta ctggccgtgc tggcgctgta gttgctgccg
    12421 tcttcacggg ttgcggtgac ggactggctg ggcagctcaa ctgggctggt cgttgcggcg
    12481 gcgaggctgc tggcggcagg catcaaggcc agggcgagca gggtgaagcg ccaggctggc
    12541 tgggcggagg agaagcgggt ggagggcatg tgctactcga ttacggatgt ttttgagaat
    12601 cattattcta ttgttctcgt cccgcttctg gatagctccg cgcagttatg ttgttgtttg
    12661 cgttgagtgc aggggggcgt ctgagtataa tggcgcgctt ccaatttccc gcccgggagc
    12721 ccccgccatg ctgcgtatca gtcaagaagc tcttaccttc gacgacgttt tacttattcc
    12781 aggttattcc gaagttctgc cgaaggatgt cagcctcaaa acgcgtctga cccgtggcat
    12841 cgaactgaat attccgttgc tctctgctgc catggatacc gtgactgaag cccgtctggc
    12901 gattgccatg gctcaggaag gcggcatcgg catcatccac aagaacatga ccgtcgagca
    12961 gcaagctgcc gaagtgcgca aggtcaaacg ctacgaggcg ggcgtggtca aggacccgat
    13021 caccatcgaa gccgatgcca ccgtgcgtga tctgttcgaa ctgacccgca tgcacaacat
    13081 ctccggcgta ccggttctga gcgatggcga cctggtcggc atcgtcacct cgcgtgacgt
    13141 gcgtttcgag aaccgcctgg acgccaccgt gcgtgaagtg atgacgccaa aagagcgtct
    13201 ggtcaccgtc ccggaaggtg ccgacaaaga cctggtgcgt gagctgctgc acaagcaccg
    13261 catcgaaaaa gtcctgatcg tcgatggtgc cttccacctg aagggcatga tgaccgtcaa
    13321 ggacatcgaa aaagccaagg cttatccgct ggccagcaag gacgaccaag gtcgtctgcg
    13381 cgtcggcgca gccgttggta ctggtgccga taccgctgac cgcgtcgctg cactggctaa
    13441 cgccggcgtt gacgtgatca tcgttgacac cgcacacggt cactccaaag gcgtgatcga
    13501 ccgcgtgcgt tgggttaagc agaacttccc ggacgtgcag gtgatcggcg gcaacatcgc
    13561 caccggcgaa gccgccaagg cccttgccga agctggcgct gatgcggtca aggtcggtat
    13621 cggccctggc tcgatctgca ccacgcgtat tgtcgcgggt gtcggcgtgc cgcaaatctc
    13681 cgccgtggct aacgttgctg ccgcactggc tggcaccggt gtgccgctga ttgccgatgg
    13741 cggtatccgc ttctccggtg acctgtccaa ggccatcgtg gctggcgcgt cctgcgtgat
    13801 gatcggttcg atgctcgctg gtactgaaga agctccgggc gaagtcgagc tgttccaggg
    13861 ccgctcctac aaggcctacc gcggcatggg ttcgctgggc gccatggcgc aggcgcaggg
    13921 ttcttcggat cgttacttcc aggactcctc ggccggtgcc gagaagctgg tgccggaagg
    13981 tatcgaaggc cgcgtgccat acaagggcgc gatggcagcc atcgttcacc agctgatggg
    14041 cggcctgcgt gcctccatgg gctacaccgg ttgtgcgacg atcgaagaga tgcgcagcaa
    14101 gcctgagttt gtgcgcatca ccggcgctgg catggccgag tcccatgtac acgacgtaca
    14161 gatcaccaaa gaagccccga attatcgcgt gggctgattg ttgatcagtt gatataaagc
    14221 ccggggctgt cgctgttgtt ttacagggca gccccgagtc atttgtgaat tccgctacga
    14281 gagacggcca tggctcatca agacatccac gctcaccgca tcctgattct ggatttcggc
    14341 tcccagtaca cccagctgat cgcccgccga gtgcgcgaga tcggtgtgta ttgtgagttg
    14401 cacccctggg acatgagcga cgatgacatc cgtgccttcg ccccgcgcgg catcatcctt
    14461 gccggtggcc cggagtcggt gcatgcagcc gacagcccgc gcgcgccgaa agccgtcttc
    14521 gacctcggcg taccggtttt cggtatctgc tacggcatgc agaccatggc cgagcagctg
    14581 ggcggcaagg ttgaaggctc cgacctgcgc gagttcggtt atgcccgcgt tgatatcgtc
    14641 ggcaaagccc gcctgctcaa cggcatcgaa gaccatatcg atgacgacgg cgtgttcggc
    14701 ctcgacgtgt ggatgagcca cggcgacaaa gtcaccgcca tgccggccgg tttccatatc
    14761 ctcgccagca ccccgagctg ccctatcgcc gctatcgcgg acgacagccg cggctactac
    14821 ggcgtgcagt tccacccgga agtgacccac accaagcagg gcggtcgcat cctctcgcgc
    14881 tttatcctgg aaatctgcgg ctgtgaagcg ctgtggaccg cctccaatat cgttgaagac
    14941 gccatcgcca acgtgcgcgc ccaggttggc agcgccaatg tgctgctcgg cctgtccggc
    15001 ggtgtggatt cctcggtggt cgcggcgctg ctgcacaagg ccatcggcga ccagctgact
    15061 tgcgtattcg tcgacaacgg cctgctgcgc ctgcacgaag gcgagcaggt gatggccatg
    15121 ttcgccgaga acatgggcgt caaagtgatt cgcgccaacg ccgaagagca gttcctcggc
    15181 aacctggctg gcgaagcgga cccggagaag aagcgcaaga tcatcggccg cacctttatc
    15241 gacgtgttcg atgccgaatc ctgcaagctg gacaacatca agttcctcgc ccagggcacc
    15301 atctacccgg acgtgatcga gtcggctggc gcgaaaagcg gcaaggccca cgtgatcaag
    15361 tcgcaccaca acgtcggcgg cctgccggaa gaaatgaacc tgaaactggt cgagccactg
    15421 cgcgaactgt tcaaggacga agtgcgccgc ttgggcctcg aactcggcct gccgtacgac
    15481 atggtctacc gtcacccatt ccacggtcca ggcctgggcg tgcgcatcct cggtgaggtg
    15541 aagaaggaat acgccgacct gctgcgtcgt gctgaccata tctttatcga agagctgcgc
    15601 aaggccgact ggtaccacaa ggtcagccag gcattcgtgg tgttccagcc ggtgaaatcg
    15661 gtgggcgtgg ttggcgatgg ccgtcgttac gcctgggttg tcgccctgcg cgccgtggaa
    15721 accatcgact ttatgaccgc acgttgggcg cacctgcctt acgagctgtt ggaaaccgtc
    15781 agcggccgca tcatcaatga aatcgaaggt atctcccgcg tcacctacga cgtgtcgagc
    15841 aagccgccag cgactattga gtgggaataa atttttataa gttattgatt taaatgtgag
    15901 gtttcattgt tcgttaacta taggcctcat atcatcagtg gtattccaca tggcatgtgg
    15961 aaggccgtcc atggggaaga cactaatgcc atgtctagac gagtagtgcg gacatggtat
    16021 ctatgcggtg ctttttagag cttccttagt cagacttttc tgacgtagtc ttttcccttt
    16081 catcctcggt ggcatgaaat gtaaggttga acaccttttc cccggttcct tgaaccgtgc
    16141 attccgcttc acgtagagct gatacgggtt gattgtcctg gaatagctga aggaaatgtt
    16201 gagcaaccgc gaaggcggat cgaggtgtca ccttcgttcc gggcatacta atgttgatct
    16261 gctgctctcg gtcaaatttt tcttctggtt ctcggaattt taattttatc tcaaacatga
    16321 tttcggctcc cacgcgcatt gcgtcgtcat agtggcgttg ccctaattct ggattgaata
    16381 ttgagtctgc tgccttaaga gaaattcttg tggctattcc tacagcaaga agggccagtg
    16441 attctgctac gccttgctcg tcgggcctcc aagcatcctt tcgcttaacg tttccgtgga
    16501 aatagaaacc gcgtttctct acgagatgaa caatcacgtc gtctatcgtc ggatttgtca
    16561 ccagcagcct ggccgtatct gaggtgtggt cactccttgc cgatgtcctt tccttgataa
    16621 caaagtccaa gatgtctcgg aattcttggt tgtttttcag cgaggtttgc aaaccttcct
    16681 tcttaaattt cccctcgcca taaagagatt caataagtag gaaggagtag cgaaaagaat
    16741 taataaattc ctgtgcatca agtgcctttc gggcacttct tacgagaggg gcttcaaacc
    16801 ttgatacttg gtgcttgtca gcagccataa ttgctcgcgt aagcatatcg aaagagagtg
    16861 gaagtgcaga gtcatgccgt cctttcgaaa taccctttac ttggattttg gtttcttctt
    16921 caggtgtttc gccttcatat ttggcatcga tttcatctgt gacgagttca atgtcgtata
    16981 tgcattcgag aagtgtggct gcgtcatcga gaaggttctt cacgaatatc atgcgatgat
    17041 cgcgcccggt aattgttgtt aggggctcgc tgtcgctggg tggcttaaga cgtggtgcgt
    17101 attcaagggg ttgtttttcg aaggtgattt caagagcttt ggcgtagcca gcctcctcga
    17161 tgacgcgaag cttcccgcct tgaattggaa tttgccagtg gtcgtcgatg atgatcttgc
    17221 gcttgaatgg aactgtgtaa cgaattctca tggtggcctc cttcgcagag aacatgtccg
    17281 gggttggccc ttggacggaa cattgtcacg catattccag ttagcctttc cccggtgttg
    17341 tggtcaacga actctggaca ctggtttagg tttatcccgc cagtgattcg gcttctactg
    17401 gcgttttgta tccgttatag ctatgtggcc gttgatggtt gtagtcgtcc atcagatagc
    17461 gcagcacgtc cgcctcagcc tgccttggtt ggtatagcca tgctctggca tccactccgt
    17521 tttcaggctt cttaagaatc gttccattgg tgcgttatcc cagcagctgc cacggcggct
    17581 catgctctgc atgatccggt agcgccacag catttggcgg aactcgatgc tggggcgtaa
    17641 tcactgcctt ggtcagagtg aaacatgccc ttgtcgtttc tgctgcggca ttctgtccgc
    17701 ttttcggggt cgtcagttcc gttgcttaaa tgagaagata ttgcaattgg tagtccgggt
    17761 taactccgga ttgatatctt cccggattgc atccgggcta cgggaactcc tatgtccttt
    17821 acccgcagac aaatgctggt cggtctggct ggtcttggtg tcgttggcct tggcaccggc
    17881 ggcgtgcgct actggctggg tcgcccggaa aatgtagcga cccacgacta tgaactgatc
    17941 gccgcgccgc tggatatcga actggtgccg gggcatacca cgccggcctg ggcctatggc
    18001 ggtcaggcac cgggtgtgga gttgcgctgt cggcagggcg agcgcttgcg tgtgcgcttc
    18061 attaataggc tggacgtgcc gaccactatt cactggcatg gcattcgtct gccgctggaa
    18121 atggacggcg tgccttatgt gtcgcaggcg ccggtgctgc cgggtgagta cttcgactac
    18181 aacttcatca cccccgatgc cggcagcttc tggtatcacc cgcacgtcgc cagcggcgag
    18241 cagctggggc gcggtctggt cggtgcgttg atcgtcgaag agcgcgagcc gacgggtttt
    18301 cgtcacgacc acacgcttaa tctgaaaagc tggcatatcg acgaacaggg cgcatttact
    18361 gcattcagcg taccgcgcga ggcggcgcgt ggcggcacac cggggcgctt gagtacggtc
    18421 aatggcaagc atgcaccaac cctggatttg cctgccgggc aggtggtgcg cctgcgcctg
    18481 atcaatctgg ataacaccct gacttatcgt ctcaacctgc ccggtgcgga tgcgcgcatt
    18541 tatgcgctgg acggcaaccc ggtcgagccg cggccgctgg gcaaggcgta ctggctgggg
    18601 ccgggcatgc gtatcgagct ggggttgaag gtgccggcgg cgggtacaga gttgtcgctg
    18661 cgtaacggtc cattgcgcct ggcgaccctg cgcgcgcagc ccggtgtcga ggtggcgggc
    18721 gagtggccgt cagccttgcc ggccaacccg gtggccgagc cggatctggc tgctgccgaa
    18781 accctgcgct tcaacttcga gtgggccggt gcggtgtcgg tcggtttggc ccagggcgcg
    18841 gcgcacagct tctggcagat caatggccag gcctgggata tcaatgacaa gacctgtgcc
    18901 gagcggccga ttgcccgcct gcagctgggc aaaagctata tcttcgagct gcgcaacctt
    18961 agccagtatc agcacccgat ccatctgcac ggcatgacct tcaaggtgtt ggcgtccaat
    19021 cgcaaggcgg ttattccgta tttcaccgac acctacctgc tgggcaagaa cgaaagtgca
    19081 cgggtggcgc tggtcgccga taatcccggg gtgtggatgt tccactgcca tgtgatcgac
    19141 catatggaaa ccggcctgat ggcctccatc gaggtggtgt gagcgttccc gtagggtgcg
    19201 cggtgcgcac cggtgcaagg gttggcccga cgagactatg aaaaaagtac agatcatcga
    19261 tcgcagtcgc gattcgtttt ttatgcgtga ggctctggcc ctggcggccg agggtgcggc
    19321 cctgggcgag gtgccggtcg gtgcggtgct ggtgcaggat ggcgcgatca tcgggcgcgg
    19381 tttcaattgc ccgatttcca gtcacgatcc cagcgcccat gccgagatgg tggcgatacg
    19441 tgccgccgcc cgggccgtgg acaactaccg tctgccgggc agcaccctgt atgtgacctt
    19501 ggaaccgtgc agcatgtgcg ccgggctgat cgtgcatgcg cgggtgcagc gtgtggtgta
    19561 tggcgcaacc gagcctaaag ctggggtggc gttgagtcgc ggacagtttt tcagccagga
    19621 gtttctcaat catcgggtgt tgattgaggg cggtgtactg gcggaggagt gtggggcggt
    19681 gctgagtgcg ttcttcaagg cgcggcgcga gcagcgctag aacctatcca aagtctgctg
    19741 cgcgtcggcc ctacggcgtt aaaaccaggc tcggaatgct catttacatc agtaaactcc
    19801 gcttcctcgc ctgtttttgc cttgcaggac tctagctcgc gcgcctttga agtaggttct
    19861 gttaaagcgg cggggtctgc tcattcggct gacgtttatc gatgcccggc acatgctgat
    19921 tgctttcggc gatttgcgag ccttccaatt gcggctgggt tacccaggta aggatgtcgt
    19981 agtagcggcg gatgttgcgc acaaagtgca ccggttcgcc gccgcgggca tagccgtagc
    20041 gggtcttgct gtaccactgt ttctgcgaca ggcgcggcag aatcttctgc acatccagcc
    20101 atttattcgg gtccaggcct tcggcttcgg tgagcttgcg cgcatcttcc agatggccgc
    20161 cgccgacgtt gtaggacgcc agggcgaacc aggtgcgatc cggctcctga atgctttccg
    20221 gcagctggtt cttcacatag acaatgtatt tggcgccgcc ttcgatgctc tgtttggggt
    20281 tcagtcggtc cgacacgccc atggcctggg cagtgcgcag ggtcagcatc atcagcccgc
    20341 gcacaccggt tttcgaggtg gcggtgggtt gccagagtga ttcctgataa cccatggcag
    20401 cgagcaggcg ccagtcgacc tgattggcct gagcggcttc gcgaaagtgt ttctcgtagc
    20461 gcggcagacg ttgttgcagg tgtttggcga aggtgtaagc gccgacatag ccgagtacat
    20521 cgacatggcc gtagtagcga tctttcaacc gctgcaggct gccattctgg tgggcgcgct
    20581 cgataaaggc attcatctcg tcgagcaggc tgcggtcttc gccgggggcg gtggcccagg
    20641 ccaggttttg tgcatcgccc agatcgaagg ccacgcgcac gttggagaag tacacctggt
    20701 tcatcgccag ttcgttggag tcgaccaggg tcaggtcgat ctgcccttca tcgaccatgc
    20761 gcagcaggtc gaccacttca acggcagcgg attcttcgta aaccagttcc ggcaattgca
    20821 gcttgagcgc ggctagctgt tcggcatggc tgctgccttt aagtaccaga atgcgcttgc
    20881 cgagtagatc atccgggcgg gtcgggcgtt gctggccgtt gcgataaatc acctggggag
    20941 tgacttcgag atagggcagg gagaagcgtg cgtgttgctg ccgtccgtcg ctttctacca
    21001 ggccggcggc agccagtacc gggccgttgg gtttgttcag gctggcgaac agctcgtcga
    21061 ggttgtcggc ggtttcgatt ttcagctcaa ggcccaagtc atcggcaaag cgtttcacca
    21121 gctcgtattc gaagccggtc tcgccgttac ggtcctggaa ataggtggcc gggctgttac
    21181 gggtgatcac ccgcagtaca ccctccgcct gaacgcgctc gagtgtgctg ggttcttcag
    21241 cacagccgcc gagcaccagg aggattccga tcaccgacag ccagcaggcg cagcgcacac
    21301 ggaacgcaga ttgggcaaac
//
LOCUS       DASRUM010000066         3703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1373987, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000066 DASRUM010000000
VERSION     DASRUM010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373987"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1848)
                     /gene="dgcA"
                     /locus_tag="VFF22_04680"
     CDS             complement(<1..1848)
                     /gene="dgcA"
                     /locus_tag="VFF22_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylglycine demethylation protein DgcA"
                     /protein_id="HZX16150.1"
                     /translation="MAFEAMFQPIQIGKLTIRNRIVSTAHAEVMATDGGMTTERYVKY
                     YEEKAKGGVGLAICGGSSVVSIDSPHGWWSSVNLSTDRIIPHFQNLADAMHKHGAKIM
                     IQITHMGRRSRWDGFDWPTLMSPSGIREPVHRATCKTIEPEEMWRVIGDFAKAAARAK
                     AGGLDGVELSAVHQHMIDQFWSPRVNKRTDEWGGTFEGRMKFGLEVIKAVRAEVGADF
                     CVGLRISGDEFHPDGLSHEDMKQIAAYYDATGLIDFIGVVGSGADTHNTLANVIPNMS
                     YPPEPFLHLAAGIKEVVKVPVLHAQNIKDPNQATRILEGGYVDMVGMTRAHIADPHLI
                     AKIKMGQVDQIKQCVGANYCIDRQYQGLDVLCIQNAATSREYMGLPHIIEKTSGPKRK
                     VVIVGGGPAGMEAARVAAERGHDVTLFEKKDSLGGQITLAAKAPQRDQMAGITRWFQL
                     ELARLNIDLRLGTGADEASILDLRPDIVVLANGGHPFIEQNEHWGAAEGLVVSSWDVL
                     DGTVAPGSNVLVYDTICEFGGMSVADFIADKGAKVEIVTDDIKPGVAMGGTTFPTYYR
                     SMYPKEVIMTGDMMLEKVYREGDKLVAVLENEYTGAKEERVVDQVVVENG"
     gene            complement(1945..2475)
                     /locus_tag="VFF22_04685"
     CDS             complement(1945..2475)
                     /locus_tag="VFF22_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5943 domain-containing protein"
                     /protein_id="HZX16151.1"
                     /translation="MATHAPEMPIQVDDETGVWTTDALPMLYVPRHFFVNNHMGIEEV
                     LGAEAYAEILYKAGYKSAWHWCEKEAECHGLSGVAVFEHYMKRLSQRGWGLFETQKLD
                     LETGYAEVKLKHSAFVYVYGKVGRKVDYMFTGWFSGAIDQILAAAGSPIRTVTVQEYG
                     GSEEGHDDGLFVVKPL"
     gene            complement(2662..3639)
                     /locus_tag="VFF22_04690"
     CDS             complement(2662..3639)
                     /locus_tag="VFF22_04690"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HZX16152.1"
                     /translation="MSPAELHADSIVIDGLIIAKWNRELFEDMRKGGLTAANCTVSVW
                     EGFQATVNNIAASQKLIRENSDLVMPVRTTADIRKAKEQGKTGILFGFQNAHAFEDQI
                     GYVEVFKQLGVGIVQMCYNTQNLVGTGCYERDGGLSGFGREIVAEMNRVGIMCDLSHV
                     GSKTSEEVILESKKPVTYSHCLPSGLKEHPRNKSDEELKFIADHGGFVGVTMFAPFLA
                     KGIDSTIDDYAEAIEYVMNIVGEDAIGIGTDFTQGHGQDFFEYLTHDKGYARRLTSFG
                     KIINPLGIRTVGEFPNLTETLLKRGHSERVVRKIMGENWVRVLADVWGE"
BASE COUNT          651 a         1142 c         1111 g          799 t
ORIGIN      
        1 gccgttttcc accaccacct gatcgaccac gcgctcttct ttcgcgccgg tgtattcgtt
       61 ctccagcacc gccaccagct tgtcgccttc gcggtagacc ttctccagca tcatgtcgcc
      121 ggtcatgatc acttctttcg gatacatgct gcggtagtag gttgggaacg tggtgccgcc
      181 catggccaca cccggcttga tgtcgtcggt gacgatctcc actttcgcgc ctttgtcggc
      241 gataaaatcg gccaccgaca taccgccgaa ttcgcaaatg gtgtcgtaca ccagcacgtt
      301 actgccgggt gcgacggtgc catcgagtac gtcccagctg ctcaccacca ggccttcagc
      361 ggcaccccag tgttcgtttt gctcgataaa cggatgaccg ccgttggcca gcacgacgat
      421 atctgggcgc aggtcgagga tgctcgcttc atcagcacca gttcccaggc gcagatcgat
      481 attcaggcgc gccaactcca gctggaacca gcgggtgatg ccggccattt ggtcgcgctg
      541 cggcgccttg gcggccaagg tgatttgccc gccgaggctg tctttcttct cgaacagggt
      601 cacatcgtgg ccacgctcgg cagccacgcg cgccgcttcc atcccggccg ggccaccgcc
      661 aacaatcacc accttgcgct tcggcccgct ggtcttctcg atgatatgcg gcaggcccat
      721 atattcacgg ctggtcgcgg cgttctggat acacagcacg tcgaggcctt gatactggcg
      781 gtcgatgcag tagttggcac cgacgcactg cttgatctgg tcgacctggc ccattttgat
      841 cttggcgatc aggtgcggat cggcgatatg cgcgcgggtc ataccgacca tgtcgacata
      901 gccgccctcc aggatccgcg tggcctggtt cgggtccttg atgttctgcg cgtgcagcac
      961 cggcaccttg accacttcct tgatcccggc cgccaaatgc aggaacggct ccggcgggta
     1021 gctcatgttg gggataacgt tggccagggt gttgtgggtg tcggcaccag agccgaccac
     1081 gccgataaag tcgatcaggc cggtggcatc gtaatacgcg gcgatctgct tcatgtcctc
     1141 gtgagagagg ccgtccgggt ggaactcgtc accggagata cgcaggccaa cgcagaaatc
     1201 ggcaccgacc tcggcgcgca ccgctttgat cacttccaga ccgaacttca tccggccttc
     1261 aaaggtgccg ccccactcgt cagtacgctt gttgacccgc ggcgaccaga actggtcgat
     1321 catgtgctgg tgcacggccg acagctcgac gccgtccagg ccgccggctt tggcccgtgc
     1381 cgccgccttg gcgaagtcac cgatgacgcg ccacatttcc tccggctcga tggtcttgca
     1441 ggtggcacgg tgtaccggtt cgcggatacc ggacggcgac atcaaggtcg gccagtcgaa
     1501 accatcccag cgcgagcgac ggcccatgtg ggtaatctgg atcatgatct tggcgccatg
     1561 cttgtgcatg gcatccgcca ggttctggaa gtgcggaatg atccggtcag tcgacaggtt
     1621 caccgaactc caccagccgt gcgggctgtc gatggagacc accgacgagc cgccgcagat
     1681 ggctaggccg acaccgccct tggccttctc ttcgtagtac ttgacgtaac gctcggtggt
     1741 catgccgccg tcggtggcca tgacctcggc gtgcgcggtg gacacgatgc ggttacgaat
     1801 agtcagcttg ccgatttgga tcggctggaa cattgcttcg aaagccatta cggcatcctc
     1861 acgaaatcgg cgagttgtcg taggagcggt aggggcacct tagccccatg gccgcgattc
     1921 gcgggcatgg cccgctccta caaatcaaag aggtttcacc acaaacaggc catcgtcgtg
     1981 gccctcttcg gaaccgccgt attcctgcac ggtgacggtg cggatagggc tgcccgcagc
     2041 ggcgagaatc tggtcgatgg cgcctgagaa ccagccagtg aacatgtagt ccaccttgcg
     2101 ccccaccttg ccgtacacgt agacgaaggc cgagtgcttg agcttgacct cggcatagcc
     2161 ggtttccagg tccagcttct gcgtctcgaa cagcccccag ccgcgctgcg acaggcgctt
     2221 catgtaatgc tcaaacaccg ccacaccaga cagtccgtgg cattcggcct ctttctcgca
     2281 ccagtgccag gcagacttgt agccggcctt gtagagaatc tcggcatagg cctcagcgcc
     2341 gagcacttcc tcgataccca tgtggttgtt gacgaagaaa tgccgcggca catagagcat
     2401 cggcagggcg tcggtggtcc agacgccggt ttcgtcgtcg acctggatag gcatttcggg
     2461 tgcgtgggtg gccatgtgtt cagtgctcca gaattcggtg gtggtgcttg atgggtatcg
     2521 cttcgctcaa cccatcctac gaaggtgttt ctgatcgttc ccacgctctg cgtggtaacg
     2581 cctcacagga cgctctgcgt ccgcttctgt gacgcaaagc gtcaggggct ggggaacgat
     2641 tatcgtgtta tggctagacg cttactcgcc ccagacgtcc gccagaaccc ttacccagtt
     2701 ctcgcccatt atcttgcgca ccacgcgttc ggaatggccg cgtttgagca gggtctcggt
     2761 gaggttgggg aattcgccga cggtgcggat gcccagcggg ttgatgatct tgccgaagct
     2821 ggtcaggcgg cgggcgtagc ccttgtcgtg ggtcaggtac tcgaagaagt cctggccgtg
     2881 gccctgagtg aagtcggtgc cgatgccgat ggcgtcttcg ccaacgatat tcatcacgta
     2941 ctcgatggct tcggcgtagt cgtcgatggt cgagtcgatg cctttggcca ggaacggcgc
     3001 gaacatggtc acgccgacga agccgccgtg atcggcgata aatttcagtt cttcatcgga
     3061 tttgttgcgc ggatgttctt tcaggcccga cggcaggcag tgggagtaag tgaccggttt
     3121 tttcgattcg aggatgactt cttctgacgt cttggaaccg acgtgggaca ggtcgcacat
     3181 aatgccgacg cgattcatct cagcgacgat ctcgcggccg aagccggaca gcccaccgtc
     3241 acgctcgtag cagccggtgc cgaccaggtt ctgggtgttg tagcacatct gcacgatgcc
     3301 cacgccgagc tgcttgaaca cctcgacata gccgatctgg tcctcgaacg catgggcgtt
     3361 ctggaagccg aagaggatgc cagtcttgcc ctgctctttc gccttgcgga tgtcggcggt
     3421 ggtgcgcacg ggcatgacta ggtcgctgtt ctcgcggatc agcttctggc tggcggcgat
     3481 attgttcacc gtggcctgaa agccttccca caccgacacg gtgcagttgg ccgccgtcag
     3541 gccgcccttg cgcatgtctt caaacagttc gcggttccac ttggcgatga tcagcccgtc
     3601 aataacgatg ctgtcggcgt gtaattcggc tgggctcatc aggcagtccc ctttttagcg
     3661 gtgtcgccaa acccgttgtt ggcgctttgg actgagcata tcc
//
LOCUS       DASRUM010000067        17560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_869959, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000067 DASRUM010000000
VERSION     DASRUM010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 17560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17560
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869959"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VFF22_04695"
     CDS             <1..834
                     /locus_tag="VFF22_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZX16153.1"
                     /translation="QDPHSQRLMKVIDLWDKPLPEQKIKDARRAYFGACSYIDSNIGK
                     LLKTLNDCNLSDNTIIVFSGDHGDMLGERGLWYKMHWFEMAARVPLLVHAPKQFAARR
                     VSQNVSTMDLLPTLVELAGGTLQADLPLDGRSLLAHLEGRQGHDEVIGEYMAEGSISP
                     LMMIRRGEWKFIYSELDPLLLFNLRHDPQERENLAEAPEHQSLLADFINQAQARWDMP
                     AIHQATLASQRRRRFVASALSHGRLASWDHQPMVDASQQYMRNHIDLDDLERRARFPQ
                     P"
     gene            889..1812
                     /gene="choX"
                     /locus_tag="VFF22_04700"
     CDS             889..1812
                     /gene="choX"
                     /locus_tag="VFF22_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015220 - choline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015871 - choline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline ABC transporter substrate-binding
                     protein"
                     /protein_id="HZX16154.1"
                     /translation="MHIFSTALIGGLLLTASSYSLAEDASCATVKLGDPGWSDIAVTN
                     GIASFLLDGLGYKAQPQTLAVPIIFAGLQKGQVDVFLGNWMPAQQSNYDKFVATGEVE
                     QVVENLSGTEYTLAVPAYAYEAGVKTFADLDQHADKFKHKIYGIGSGAPANESIQKMI
                     AADEFGLKDWSLVESSEQAMLVQVGRAVKREQFVVFLGWTPHPMNVQYDMRYLKGGEK
                     YFGASGSVNTLARKGYAAQCPNVGKLLSNLKFTQEIENAIMDQVLSKQASNDEAIKAW
                     LKANPAVVAGWLEGVTSRNGGDGLAAVNAKL"
     gene            1883..3448
                     /locus_tag="VFF22_04705"
     CDS             1883..3448
                     /locus_tag="VFF22_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HZX16155.1"
                     /translation="MPLLRLQTLLPFLRWLPHCSKRSLGKDLLVGLNGAILALPQSIA
                     YALIAGLPPQYGLYAAIVPVLIACLWGSSSFLICGPTAAISIVLFSSVSPMATPGSDH
                     FIALILLLTLLAGVCQWLLGLLRFGALVNFVSHSVVLGFTLGAALVIGIGQLPNLLGL
                     TVTSQATALMSLLRLGEHLAESDWRTLAVALFTLSISLLSRRLWPRAPALLIGIVAAS
                     LLVMAWPEQLGAIALVSRFEGSLPPFALLDFDLTNILQLLPAAVACGLLGLVTSLSIA
                     RALADKSQQPFDANQEVRAQGLSNIVGPWFSGSLSAGSFTRSALNLQAGACSPMAGVF
                     AALLVALFALYGSRLFAYVPIPAMAASILLICWGLFDIGAVKALQRVSRSEFLVMLLT
                     VLATLVLDLQSAIYAGVLASLIVYLKRTSQPRVRQWQDADEEWLRIEGSIFFGACHYL
                     QQMLQRSTARWVMIDAQHINFIDYAGITMLHQEARRLHSQQRHLVLHHARPQVIEEIR
                     KLEGPERCPVQFA"
     gene            3613..4626
                     /gene="cysP"
                     /locus_tag="VFF22_04710"
     CDS             3613..4626
                     /gene="cysP"
                     /locus_tag="VFF22_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate ABC transporter substrate-binding
                     protein CysP"
                     /protein_id="HZX16156.1"
                     /translation="MLKKLLLASAITSSLLTSAATLAQDKPILNASYDIARELFAEIN
                     PKFVEHWKAQTGKELKIDQSFAGSSRQAQDIIQGKKVDTVTFNQVTDVDILAKRGLVR
                     EDWAEQFPNNSSPYYSTTAFLVRKGNPKNIKGWDDLARDDVKLVFPNPKTSGNARYTY
                     LGAWLHANEAFKGDETKTKEFVGKILRNVENFPTGGRAATVAFAQNGQGDVLLTFESE
                     VVNIANGEEFKSGEYEIVVPPVSVLAEFPVALVDKVVDEKGTRDVSKAYLDFQYSKDI
                     QQLLTTYSYRVHHPEVVAATAEQFPKIRLINPTEVLGTWDEITAKHFDADGILDQLLA
                     EGR"
     gene            4696..5544
                     /gene="cysT"
                     /locus_tag="VFF22_04715"
     CDS             4696..5544
                     /gene="cysT"
                     /locus_tag="VFF22_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HZX16157.1"
                     /translation="MPKTPRFFLQNPLLPGFGLSFGFSTFYISLIILFPLSALLLYVS
                     DMSWAQYWRAISDPRVVQSYKVTISGAFYATIATVLIGMLLAWIITRYDFPGRRLVDA
                     LVDLPFALPTSVAGLTLATLLAPGGWLGQWLAPMGIKLAYAYPGILIAMVFTSLPFVV
                     RTVQPVLQDLGRDYEEAASTLGANRLQTFFYVVLPTLTPALVTGASQSFIRSLGEFGA
                     VIMIAGNIPFKTEVSSLMIFVRLQEFNHNAAAAIASVILLASLVLLFSLQVLQGRLFK
                     WQRQGK"
     gene            5541..6368
                     /gene="cysW"
                     /locus_tag="VFF22_04720"
     CDS             5541..6368
                     /gene="cysW"
                     /locus_tag="VFF22_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012020091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HZX16158.1"
                     /translation="MRKPQHWHQWLLIGLGLSLVTLILLIPLALIFSKAFSGGLELLW
                     GNLGEDYMLHAIGLTLLVAALTVPVNLCFGIMLAWCVTHYDFRGRKLLTTVIDIPYAV
                     SPVVAGLCYLVVYGLEHALGRWFYDNGMQLMFAWPGIVMVTIFVTAPYVARILIPVMQ
                     AQGQDEETAALCLGANGWQIFRHITLPKIKWALLYGVVVTNARAVGEFGAVSVVSGTI
                     INQTLTLPLLVDQLNNDYKPAAAFTAAALLACMALLTLLLKTFMEWRQRTLMQRSEA"
     gene            complement(6553..7281)
                     /locus_tag="VFF22_04725"
     CDS             complement(6553..7281)
                     /locus_tag="VFF22_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HZX16159.1"
                     /translation="MSMIEPVLLTAARVCTFVGQQLLTNASGFYFARDERLFLVTSRH
                     VMIDEPSQHFPDRIEIEVHTDLHNVAQTTGFSMPLYRDGKSLWRQGCDGAGEVDVAVL
                     EIERAALPKGMVYHAFTPEHLLQPQTPVEVGTSLLVVGFPLGFHDTLHHIAVVRHAVV
                     ASSFGLRFQGQGYFLTDARTHRGTSGAPVVMRTPENVDGELPWVLLGVHSARLDLGSR
                     DLELDEALGLNCTWYADILLTLTQ"
     gene            7535..7750
                     /locus_tag="VFF22_04730"
     CDS             7535..7750
                     /locus_tag="VFF22_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein CsbD"
                     /protein_id="HZX16160.1"
                     /translation="MSRPLAEIIQKNWRKLAGLALIVWDELTLDERIKSEGDEQKLTN
                     LVQQRYDMPREDASKQVMSFFERHRTS"
     gene            complement(7763..8335)
                     /locus_tag="VFF22_04735"
     CDS             complement(7763..8335)
                     /locus_tag="VFF22_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16161.1"
                     /translation="MSRTVFQAVIICVAALCSSTALAKVEVQAGQHKTLGRVLLAKVS
                     EDIAPGDYEALLKGIKANPGKYAKKIVMLDSIGGSVAEAIRMGRLLRETGFDALVPST
                     GVCQGSCVYLLAAGHAKTVRGYVGIHRPYYAGSDSVHSASAFGPSRATQAAYFKEMQI
                     PPELLAAINSTEPRRMRVLTPVELARYRLK"
     gene            8510..9046
                     /locus_tag="VFF22_04740"
     CDS             8510..9046
                     /locus_tag="VFF22_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009044782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3087 domain-containing protein"
                     /protein_id="HZX16162.1"
                     /translation="MFEIQQRTPETYRQQTRRSTLTIAGIFVALAMLLSSSAVLLFGT
                     PGGDNFRWNLGGVIAALALTVALVRVLLWPQPFMAAAVYGWRLKRSLMRITNLMHHVK
                     AGVAARDPAAMQLLRFYHLGLTQMHQLDGNSSALSEAVAEINQHKERMEELGMDADQP
                     RLNPDWLEAVKKIQPLPR"
     gene            9086..9733
                     /locus_tag="VFF22_04745"
     CDS             9086..9733
                     /locus_tag="VFF22_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-D-aspartate receptor NMDAR2C subunit"
                     /protein_id="HZX16163.1"
                     /translation="MLRHIMKPLINPPHGCLTHSWQRAWRNLNLPAPAGLFERLLAAY
                     NEPHRHYHTQQHLVECLEHFDAALALAAEPGEVEIALWFHDAIYALRGSDNELRSADW
                     AVQELALGGATTQVQARVHGLIMATRHNATPSDADQQLLVDIDLAILGAAPERFSEYD
                     RQVRAEYNWVPGLLYRIKRKAVLKHFVARNHLYSTSHFRDRYEQQARVNLRAAIG"
     gene            10182..11357
                     /locus_tag="VFF22_04750"
     CDS             10182..11357
                     /locus_tag="VFF22_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16164.1"
                     /translation="MDILFKHPHSIVLIQEHRRLQNKTDENMSKKSTSLKSIAATISM
                     IFATYASGFQLSPEGTIAEQQMASKYGVKYKTQIIIMDSSMHKFADPAHEALTQKSFG
                     CDGDWDDCDNPDLENAGPYIIAGVRWNDDPVFMLSSDDGKIGNCNTKFTVGFITQTRC
                     WVGIFKSAEKQSRTDSTRFMGKGNYMTRSHFGDLQFLHAMASQDGESADQTKQKIMMW
                     AEFTWGVVEGKYKLNTYLSDIEIPGWDQHFTNDHTVQDLFTVGRPWLRPHIKEMAIGS
                     LLHLVQDSFAAGHVNRRDAISGDQCTTNKHPTYGRIKEFHSYSKQDHSKHKEEDASSV
                     ARMTMMKNDPDMVDAGKRLRGYFADQKSWEEVQPYLNECVFALNRTTKPASAGTDYM"
     gene            complement(11412..11867)
                     /locus_tag="VFF22_04755"
     CDS             complement(11412..11867)
                     /locus_tag="VFF22_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16165.1"
                     /translation="MKERMVFGIKIEHGVTKRGVIIFWSVAVSLAIYIMSLPLNMKDS
                     SLVMNYFIFVMMMFGGGLAYHRITLMIECPQTEDNYGAWLDLVKVLIKAYMGFCFSAM
                     CVGFGVAIKGVLGFLLAVVGGFAGMIWVFNRMIDSHKYITALIDGSAQE"
     gene            complement(11971..12729)
                     /locus_tag="VFF22_04760"
     CDS             complement(11971..12729)
                     /locus_tag="VFF22_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16166.1"
                     /translation="MKSDWDDAPDYIRNNNKQSPWRTVVILGVGSAITWGLIALFAKP
                     IVIDVNQLKQAIRVNGQPLFSELPVQAQAPAYSEPAAPIRLPSLSLEPAQPVVQPPAS
                     KAEIEWVERMEAIATERAKNSFNDNNYRPKQPANTYTPPATQIASAPTVSERKSRQVS
                     QERTAKWIKSWNGGTNYLAEWVAVNNYINSSSVCANHRKGSIDYRECRKAAKQHYHEE
                     CRAWRARYDNDRKEHSDRMKQRYCSASSSFSPMG"
     gene            complement(12788..13468)
                     /locus_tag="VFF22_04765"
     CDS             complement(12788..13468)
                     /locus_tag="VFF22_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16167.1"
                     /translation="MELMAVVIAVSGALVGWVLAQFTAMAKDYLYRKRLRKLLIMELE
                     ELHDDIKGTIDAYTRQLHIYGQGGVSNEGPASISSFIFKNYYKDAVLGLSKSERKSFK
                     IIHAALEAVNEDIDAYLQETRKIQEMLSIEGVESLSKERFDYWGGLIKTGYRGCGSLV
                     WSISHHLDNLKNPEVVPMSDAHAEYLKHLDLVDHKMFHEIEAGKTIDREFFKKNYSHE
                     DFERIRGK"
     gene            14177..14266
                     /locus_tag="VFF22_04770"
     tRNA            14177..14266
                     /locus_tag="VFF22_04770"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14211..14213,aa:Ser,seq:cga)
     gene            complement(14371..14655)
                     /locus_tag="VFF22_04775"
     CDS             complement(14371..14655)
                     /locus_tag="VFF22_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020750530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZX16168.1"
                     /translation="MKVIWTPEALQDRLDIWEYIAADSTRAAVHMDELFSAAASTLAD
                     YPQKGRLGTVAGTRELIPHENYRLVYQTETDAVWILALVHVARMWPPLQG"
     gene            complement(14652..14930)
                     /locus_tag="VFF22_04780"
     CDS             complement(14652..14930)
                     /locus_tag="VFF22_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007957667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HZX16169.1"
                     /translation="MAKEAVFNLKLEPELREEFMAAAQAAHLPASQVMRDLMRDFIRQ
                     QQQAKEHDEFVQRKVAVARASVEAGRGRSNDDVEAEFAARRAKAQGHE"
     gene            complement(15026..15232)
                     /locus_tag="VFF22_04785"
                     /pseudo
     CDS             complement(15026..15232)
                     /locus_tag="VFF22_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364018.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(15373..16242)
                     /locus_tag="VFF22_04790"
     CDS             complement(15373..16242)
                     /locus_tag="VFF22_04790"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HZX16170.1"
                     /translation="MSTATTLSLKKIYSGKVRDLYEIDDKRMLMVATDRLSAFDVILN
                     EPIPEKGKILTAISNFWFDKLKDLVPNHFTGDKVEDIVPAAELPLVEGRAVVAKRLKP
                     VAVEAIVRGYIVGSGWKEYQKSGTVCGIQLPAGLKEAAKLPQPIFTPSTKAAVGDHDE
                     NISFEQCEAIIGAELAAKVRDTSIALYSAAVEYAATRGIIIADTKFEFGLDEDGTLTL
                     MDEALTPDSSRFWPADSYAEGTNPPSFDKQFVRDWLESTGWNKEPPAPAVPADVAQKT
                     ADKYREALTRLTV"
     gene            complement(16330..17448)
                     /gene="bamC"
                     /locus_tag="VFF22_04795"
     CDS             complement(16330..17448)
                     /gene="bamC"
                     /locus_tag="VFF22_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007941449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HZX16171.1"
                     /translation="MKRLTGLSALALIIAGTSGCGWIYGENGYFRDRGSDYLEARQTA
                     TMQIPPNVDAKRIDPLLPVPQQVATTTDTGEFIVPRPQALAVAGDASEFSLQKSGESR
                     WVVAQRVPAEVWPVARQFFADNGFQIAEERPQTGEFSTTWQRFDALSESMARRLSSRV
                     SGVNPDAETRVRVRIEPGVQRNTSEIFVVSAERPVGSTADVAFTNRSSNPSLDAALLD
                     EMLVTLARSAEQGGSVSLLAARDYDAPNRVSLSEDGNGNPVLSLGADFDRAWSGVGRS
                     LEMADVRIDDINRSLGVYYINLAERAQKPDEKPGFFGSVFGSEPSKEEIDARAERYQV
                     RLTAVGDSVQVTVEKDLNTIAPADVARKVLDLIQENLG"
     gene            complement(17466..>17560)
                     /locus_tag="VFF22_04800"
     CDS             complement(17466..>17560)
                     /locus_tag="VFF22_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003317384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZX16172.1"
                     /translation="PDGIRLPLTWLSPRCHEPLRQALRQSGVLV"
BASE COUNT         3888 a         5224 c         4704 g         3744 t
ORIGIN      
        1 caagacccac actcgcaacg cctgatgaaa gttatcgacc tgtgggacaa gccgctgccc
       61 gagcagaaga tcaaggacgc ccgccgcgcc tacttcggcg cctgcagcta tatcgacagc
      121 aatatcggca agctgctgaa aaccctcaat gactgcaacc tgagcgacaa caccatcatc
      181 gtgttctccg gcgaccacgg cgacatgctt ggcgagcgcg gcctctggta caagatgcac
      241 tggtttgaaa tggccgctcg ggtgccgctg ctagtgcatg cgcccaagca attcgccgca
      301 cgccgagtga gccagaacgt ctcgaccatg gacctgctgc cgaccctggt ggaactggcc
      361 ggcggcacgc tgcaagccga tctaccgctg gacggccgct cgttgctggc gcacctcgaa
      421 ggccgccagg gtcacgatga agtgattggc gaatatatgg ccgaaggcag catcagcccg
      481 ctgatgatga tccgccgtgg cgagtggaaa tttatctact ccgagcttga tcccctgctg
      541 ctgttcaacc tgcgccacga cccgcaggag cgcgaaaacc tcgccgaagc ccccgagcac
      601 cagagcctgc tggccgactt tatcaaccag gcgcaggcgc gctgggacat gccggcaatc
      661 catcaggcca ccctggccag tcaacgccgt cggcgcttcg ttgccagcgc cctgagccac
      721 ggccgcctgg ccagctggga ccatcaaccg atggtcgacg ccagccagca gtacatgcgc
      781 aaccacattg atctcgacga cctcgaacgt cgcgcccgtt tcccgcaacc ctgattgcaa
      841 gccactgccg cgttgatcca taactaataa ttcaaggaga cacatgccat gcacatattc
      901 agcacagcac ttatcggcgg cctgttactc accgccagca gctatagcct cgccgaagac
      961 gccagttgcg ccacggtgaa actgggcgat ccgggttgga gtgatattgc cgtgaccaac
     1021 ggcatcgcca gcttcctgct cgacggcctg ggctacaagg cacagccgca gaccctggcc
     1081 gtgccgatca ttttcgccgg gttgcagaaa ggtcaggtgg acgtgttcct cggcaactgg
     1141 atgccggcgc agcagagcaa ttacgacaag ttcgtcgcca cgggcgaagt cgagcaggtg
     1201 gtggaaaacc tcagcggtac cgagtacacc ctggcggtgc ccgcctatgc ctatgaggcc
     1261 ggggtgaaaa cctttgccga tctcgatcag catgccgaca agttcaagca caagatctac
     1321 ggcatcggct ccggcgcacc ggccaacgag tcgattcaga agatgatcgc cgccgacgaa
     1381 ttcggtctca aagactggag cctggtcgag tccagcgaac aggccatgct ggtgcaagtc
     1441 ggccgcgcgg tgaagcgcga gcagtttgta gtgttcctcg gctggacccc gcacccgatg
     1501 aacgtgcagt acgacatgcg ctacctcaag ggcggtgaga agtacttcgg cgccagcggc
     1561 agcgtcaaca ccctggcccg caaaggctat gccgcacagt gcccgaatgt cggcaagctg
     1621 ctgagcaacc tgaaattcac ccaggagata gagaacgcca tcatggatca ggtgctgagc
     1681 aaacaggcca gcaacgatga ggcgatcaag gcctggctca aggccaatcc ggcggtggtt
     1741 gccggctggc tcgaaggcgt aaccagccgc aatggcggtg acggcctggc cgcagttaac
     1801 gccaaacttt gaacctgccg gcttgattcc ctaaagtagg caggccgctg cctttttcat
     1861 cgcccgcaca agaagcccac gcatgccatt gctccgcctt cagaccctgc tgccctttct
     1921 gcgctggctg ccgcattgca gcaaacgcag cctcggtaaa gacctgctgg tggggctcaa
     1981 tggcgccatc ctcgcgctgc cgcaatccat cgcctacgcc ctgatcgccg gtctgccgcc
     2041 gcaatatggt ctgtacgcgg ccatagtccc ggtgctgatc gcctgcctgt ggggctcgtc
     2101 cagttttctg atctgcggac caaccgccgc gatatccatc gtgctgttca gcagtgtcag
     2161 ccccatggcg acgccgggca gcgaccactt tattgccctg attctactgc tgacgctact
     2221 ggccggtgtg tgccaatggc tgctcggcct gctgcgcttc ggcgccctgg tgaatttcgt
     2281 ctcgcactcg gtggtgctcg gcttcaccct cggtgcggcg ctggtgatcg gtatcggcca
     2341 gttgcccaac ctgcttggcc tcaccgtcac cagccaggca acggcgctga tgagtctgct
     2401 cagactcggc gaacacttgg cagagagtga ctggcgcacc ctcgccgtag cacttttcac
     2461 cttgagcatc agcctgttga gcagacgtct gtggcctcgc gcaccggcat tgcttatcgg
     2521 gattgtcgcc gccagcctgc tggttatggc ctggcctgag cagctggggg caatcgcgct
     2581 ggtcagccgt tttgaaggca gcctgccgcc gttcgcccta ctggacttcg accttacaaa
     2641 catcctgcaa ctgctgccgg cggcagtggc ctgcggtttg ctcggtttgg tcaccagcct
     2701 gtcgattgcc cgcgccctgg cggataaatc ccagcagccg ttcgatgcca atcaggaagt
     2761 tcgcgcccag ggcctgtcta acatcgtcgg gccttggttt agcggctcgc tgtcggccgg
     2821 ctccttcacc cgctcggcgc tcaacctgca agccggcgcc tgctcgccga tggccggggt
     2881 gtttgccgct ctgctggtgg cgctgtttgc cctgtatggc agccgcctgt ttgcctacgt
     2941 gccaatcccc gccatggcgg cgagcattct gctgatctgc tgggggctgt tcgatatcgg
     3001 cgcagtaaaa gcgctgcagc gcgtgagccg cagcgagttt ctggtgatgc tgctgaccgt
     3061 gctggcgacc ctggtgctgg acctgcaaag cgccatctac gccggcgtgc tggcctcgct
     3121 gatcgtctac ctcaaacgca cctcgcaacc gcgcgtacgc cagtggcagg acgctgatga
     3181 ggaatggctg cgcatcgagg gctcaatctt cttcggtgcc tgccactatc tgcaacaaat
     3241 gctgcaacgc agcacggcgc gctgggtgat gatcgatgct cagcacatca actttatcga
     3301 ctacgccggc atcaccatgc tgcaccagga agcgcgccgc ctgcacagcc agcagcgcca
     3361 tctggtgctg caccacgcac ggccccaggt gatcgaagaa attcgcaagc tggaaggtcc
     3421 ggagcgctgc ccggtgcagt tcgcctgata cgtcacgcac cggaaaattc ataaatggaa
     3481 tatcaatagc tttaaaagtt actttttaag aataaaaaac ttgggcagta tccctcccac
     3541 aacttggccg tgcggccagg tattcaagca aacgcctgta agacgggctc agtgacgagg
     3601 aaagtctgca tcatgctcaa gaaacttctg ctggccagcg ccatcacctc aagcttgctc
     3661 accagtgccg ccacactggc gcaggacaag ccgatcctga atgcctccta cgacattgcc
     3721 cgtgagctgt ttgccgagat caaccccaag ttcgttgagc actggaaagc ccagaccggt
     3781 aaagaactga agattgacca atccttcgcc ggctcctcac gccaagcgca ggacatcatc
     3841 cagggcaaga aggtcgacac cgtgaccttc aatcaggtta ccgacgtgga catcctggcc
     3901 aagcgtggcc tggtgcgcga agactgggct gagcagttcc ccaacaacag ctcgccttac
     3961 tacagcacca ccgccttcct ggtgcgcaaa ggcaacccga agaacatcaa aggctgggac
     4021 gacctggccc gcgacgacgt gaagctggtg ttccccaacc cgaagacctc tggcaacgcc
     4081 cgctacacct acctcggtgc ctggctgcat gccaacgaag ccttcaaggg tgatgaaacc
     4141 aagaccaagg aattcgtcgg caaaatactg cgcaacgtcg agaacttccc cacgggtggc
     4201 cgcgcagcga ccgtagcctt tgcccagaat ggccagggtg atgtgctgct gacctttgaa
     4261 tcggaagtgg tcaacatcgc caatggtgag gaattcaaat ccggcgaata cgagatcgtc
     4321 gtaccaccgg tcagcgtatt ggccgagttc ccggtggcgc tggtcgacaa ggtagtcgat
     4381 gagaaaggca cccgtgacgt gtccaaggct tacctggact tccagtacag caaggacatc
     4441 cagcaactac tgaccactta cagctaccgc gtgcatcacc cagaagtcgt ggcagccact
     4501 gcagagcagt tccccaagat ccgcctgatc aacccaaccg aagtactcgg cacctgggat
     4561 gagatcactg ccaagcactt cgatgccgat ggtattcttg accagttgtt ggctgagggt
     4621 cgctagaccc tgctggttta aggaacaaaa caagccgccg tacctacggc ggcttgtgca
     4681 tttagagatc tgtacgtgcc taaaactccc cgcttttttc tgcagaatcc gctgctgcca
     4741 ggctttggtc tgagcttcgg cttcagcacc ttttatatat cgctgatcat cctgtttccg
     4801 ctgtcagcgc tgctgctgta cgtcagcgat atgagctggg cgcagtactg gcgggcgatc
     4861 agcgacccgc gcgtggtgca gagctacaag gtgacgatca gcggggcctt ctacgccacc
     4921 atcgccaccg tactgatcgg catgctgctg gcctggatca tcacccgcta tgacttcccc
     4981 ggtcgccgcc tggtggatgc actggttgat ctgcctttcg ccctgcccac ttcggtggcc
     5041 ggcctgaccc tggccaccct gctcgccccc ggcggttggc tcggccaatg gctcgcgccc
     5101 atgggtatca aactggcgta cgcctacccc ggtattttga tcgccatggt cttcactagc
     5161 ctgccctttg tcgtgcgcac ggtgcagccg gtgctgcagg acctgggccg cgactatgaa
     5221 gaagccgcca gcaccctggg tgccaaccgc ctgcaaacgt tcttctacgt ggtgttgcca
     5281 accttgactc cggccctggt caccggcgcc tcgcaatcct ttatccgcag cctcggtgag
     5341 ttcggcgcgg taatcatgat tgccggcaat atcccgttta aaaccgaagt ctcctcgctg
     5401 atgattttcg tgcggctgca ggagttcaac cacaacgcgg ctgcggcgat tgcctcggtc
     5461 attctgctgg cctcactggt gctgctgttc agtctgcaag tgctacaagg ccgcctgttt
     5521 aaatggcaac ggcaaggcaa atgagaaaac cacaacactg gcatcaatgg ctgctgatcg
     5581 gcctgggcct gagcctggtc accctgattc tgctgatccc gctggcgctg attttcagca
     5641 aagcgttctc tggcggcctg gagttgctct ggggcaacct gggcgaagat tacatgctgc
     5701 atgccatcgg cctgaccctg ctggttgccg cgctgaccgt accggtcaac ctgtgcttcg
     5761 gcatcatgct ggcctggtgc gtcacccatt acgattttcg cgggcgcaag ctgctgacca
     5821 ccgtgatcga cattccctat gcggtgtcgc cggtggtggc cgggctctgc tacctagtgg
     5881 tgtatggcct ggagcacgcc ctggggcgct ggttctacga taacggcatg cagctgatgt
     5941 ttgcctggcc gggcattgtg atggtcacca tcttcgttac tgcaccctat gtggcgcgca
     6001 tcctgattcc ggtgatgcag gcccagggcc aggacgagga aaccgccgcg ctgtgcctgg
     6061 gcgccaacgg ctggcagatc ttccgccata tcaccctgcc gaagatcaaa tgggcactgc
     6121 tgtatggcgt ggtcgtgacc aatgcccgtg cagtgggcga gtttggcgct gtgtcggtgg
     6181 tgtccggcac catcatcaac cagaccctga ccctgccctt gctggtcgat cagctaaaca
     6241 acgactacaa gcccgccgcc gcctttaccg ctgcagcgtt gctggcgtgc atggcgttgc
     6301 tcaccctact gctgaaaacc tttatggaat ggcgccaacg caccctgatg cagcgctctg
     6361 aggcgtaggg tttgaacagt cgcggattac ccagaagaag cagaagcgta accgcgaaag
     6421 ccaggatgac gtaggcctag ttggggtcaa acgggacagc ggcggtactg caggcaaagc
     6481 cgaccgcagc cgtactctag gtgcgctgta ccgccgcccc cgagctgcag acgcagcgtt
     6541 gagcgcagag ccttactgag tcagggtcag cagaatatcg gcataccagg tgcagttgag
     6601 ccccagggcc tcgtccagct cgaggtcgcg actgcctaga tcaagccgcg ccgaatgcac
     6661 gcccagcaac acccacggca actcaccgtc cacattctcc ggcgtacgca tcaccaccgg
     6721 cgcgccactg gtgccgcgat gcgtgcgggc atcagtaaga aagtagccct ggccctgaaa
     6781 acgcaggccg aatgacgacg ccaccacggc atgccgcacc accgcgatat ggtgcagggt
     6841 gtcgtgaaaa cccaggggga aacccaccac cagcaaggaa gtgccgacct cgaccggggt
     6901 ttgcggctgc agcagatgct ccggggtaaa ggcgtgatac accatgccct taggcagcgc
     6961 cgcgcgctcg atctccagca ccgccacatc tacctcaccg gcgccatcgc agccttgccg
     7021 ccagaggctc ttgccatcgc gataaagcgg catggagaag ccggtggtct gcgcgacatt
     7081 gtgcaaatcg gtgtgcactt cgatctcgat ccgatcaggg aaatgctggc tcggctcatc
     7141 gatcatcacg tgccgactgg tcaccagaaa caggcgctcg tcacgggcga agtaaaagcc
     7201 gctggcgttg gtcagcagtt gttgaccaac aaaggtacag acgcgtgcgg cagtcagcag
     7261 cacaggttca atcatcgaca ttgcggcata tccttgagtt gacggcaagc atgccggaac
     7321 aagcccttgt ggctctaagc agagcaattg ccacgccaca taagctgcga cgcggcaatc
     7381 aggctatcgg cgaaacgcca tggcgtcggt acgcaaaccc gcataagcgc tgtagcgcac
     7441 tgactagccg tgccagctat gacaggatgg cttgagcaca attccgtact gatcgtacaa
     7501 ccggccaagg ccggccacct caggagcacc gagcatgagc aggccacttg ccgaaatcat
     7561 tcagaaaaac tggcgcaaac ttgccgggct cgctctgatc gtctgggacg agctgaccct
     7621 ggatgagcgg atcaaatccg aaggcgacga gcagaaactg accaacctgg tgcagcagcg
     7681 ctatgacatg ccccgcgagg acgccagcaa gcaggtcatg agtttcttcg aaaggcatcg
     7741 caccagttga tagcgcgcag cctcacttca agcggtagcg cgctaactcg accggcgtca
     7801 gcacacgcat gcgtcgcggc tcagtgctgt tgatcgccgc aagcagctcg ggcggaatct
     7861 gcatttcctt gaaataggca gcctgagtcg ccctgctcgg cccaaaggcg gacgccgaat
     7921 gcaccgaatc actgcccgca taatacggcc ggtgaatccc cacataaccg cgcacggttt
     7981 tagcgtggcc cgccgccagc aggtacacgc aactgccttg gcacaccccg gtggacggta
     8041 ccagggcatc gaagccggtc tcacgcagca agcgccccat acgaatggcc tcggctacgc
     8101 tgccgccaat gctgtcgagc atgacgatct tcttcgcata cttgcccggg ttggctttga
     8161 tgcccttgag cagcgcctcg taatcgcccg gcgcaatatc ttcggagacc ttggccagca
     8221 aaacccgtcc gagcgttttg tgctgcccgg cctgcacctc gaccttggcc aacgcggtac
     8281 tcgaacacag cgccgccacg cagataatca cagcctgaaa cacagtccga ctcataaacc
     8341 cggtaaatcc ttaagcgata cagtcaacgg atgggcgaag atacctgttg ccgggcaatc
     8401 gcgcataatt tgcagcgttt ggcgcatgct tcgactaacc cggcctgccc tacccaacgc
     8461 gtaagcaacg ccgttactac acgctcaagt gagaattccc gtggccccga tgttcgaaat
     8521 ccagcagcgc accccagaaa cctaccgcca gcaaacccgc cgcagcaccc tgaccatcgc
     8581 ggggatcttc gtcgccctgg ccatgctgtt gtcgagcagc gccgtgctgc tgtttggcac
     8641 accgggcggc gacaacttcc gctggaacct cggcggggtt atcgccgcat tagcgctgac
     8701 tgtggccctg gtgcgcgtac tgctgtggcc acaacccttt atggccgccg ctgtatacgg
     8761 ctggcgcctg aaacgcagcc tgatgcgcat caccaacctg atgcaccacg tcaaagccgg
     8821 cgtcgccgcc cgcgaccccg ccgccatgca actgctgcgc ttctaccacc tgggcctgac
     8881 gcagatgcac caactggacg gcaactccag cgccctcagt gaagcggtgg cggagatcaa
     8941 tcagcacaag gaacgcatgg aagaactggg gatggacgcc gatcagccgc ggctaaatcc
     9001 cgactggctt gaggccgtga aaaagatcca gccactgccc cgctaaaatt gatatcggtc
     9061 ggcatgcgct gcacggaagc actttttgct gagacacatc atgaagccac tcataaaccc
     9121 accacatggc tgcctcaccc actcctggca acgcgcatgg cgcaacctga atctgcctgc
     9181 accggccggt ttattcgaac gcctgctggc cgcttataac gagccgcacc gccactacca
     9241 cacgcagcaa catctcgtgg aatgcctcga acacttcgat gcggcgctcg ctcttgcggc
     9301 cgaacctggc gaagtggaaa tcgcactgtg gtttcacgat gcgatctacg cgctgcgcgg
     9361 atcagacaat gaactgcgca gcgccgactg ggccgtgcag gagctagctc taggtggagc
     9421 tacaacgcag gttcaggcgc gcgttcacgg cctgatcatg gcgacccgcc ataacgccac
     9481 acccagcgat gccgatcagc aactgctcgt cgacatcgac ctggccatac tcggcgccgc
     9541 gcctgaacgc tttagcgaat acgaccgcca ggtacgcgcg gaatacaact gggtgcctgg
     9601 tctgctgtac cgcatcaaac gcaaggcagt gctcaaacac ttcgttgccc gcaatcacct
     9661 ctacagcaca agccacttcc gggatcggta cgagcaacag gccagggtca atctgcgggc
     9721 ggcgattgga taagcgcaag cgaggggttg ctcgcgttaa cccagcaaaa gctgtgtacc
     9781 cgttaactgt tacgcgactg tcggcttcca ccgattagtc ccctcgccct ccgggagagg
     9841 ggctgcgcca tccatgcctc acaacatata gctgggaaat agccccaaaa gcccctgaat
     9901 ctatgacgac ttcaggggct gaataaattt cgatatggcg gacgataggg ctcttgaacc
     9961 tgcctgtgag cgcttcagtt cgtgccaaac ccgctcaata cggagcttcc gccgccatca
    10021 cctgctcgtt tcggctcaat tcggcacagt gttttcgaca ctttttcgac acattcgatt
    10081 ccagagctat cctgtctcta gtggcaaact gccacagaca caagcactca cttaacacat
    10141 gcattagcag ttaactcgcg aatttaaaaa tagcagcaat gatggatatt ttatttaaac
    10201 atcctcattc aatagtatta atacaagagc atcgacgctt acaaaataaa acggacgaaa
    10261 acatgagcaa aaaatcaacc tcacttaaaa gcatcgcagc aaccatttca atgatattcg
    10321 ccacttacgc cagtggattc cagctatcac cagaaggcac cattgccgag caacaaatgg
    10381 caagcaaata tggggtgaaa tataaaacac aaataataat tatggactcc agcatgcata
    10441 aatttgctga tcctgcacat gaggctctta cacagaaaag ttttggatgt gacggtgact
    10501 gggatgactg cgacaaccct gaccttgaaa atgcgggtcc ttatattatt gccggggtaa
    10561 gatggaatga cgaccctgtt ttcatgctaa gttcagatga tggaaaaata ggaaactgca
    10621 atacaaaatt cacagtcggt tttataactc aaaccagatg ctgggtcgga atatttaaaa
    10681 gcgccgaaaa gcaaagcagg acagactcca cccgctttat ggggaaagga aactatatga
    10741 ctcgctctca ttttggagac ctacagttcc ttcatgctat ggcctctcaa gacggcgaaa
    10801 gtgctgatca aaccaaacag aaaataatga tgtgggctga gtttacttgg ggtgtcgttg
    10861 aaggtaaata taagctcaat acctatctta gcgatattga aattcccggc tgggatcagc
    10921 acttcacaaa tgaccacaca gtacaagatc tatttacagt cggccgccct tggctgcggc
    10981 ctcatataaa agaaatggct attggcagct tacttcacct cgttcaagac agttttgctg
    11041 caggccatgt taatcgccgc gatgcaatct caggtgatca atgcaccacc aacaaacacc
    11101 ccacatatgg ccgaataaaa gaatttcact catattccaa acaagaccac tccaaacaca
    11161 aagaagagga tgcaagtagc gtcgctagaa tgacaatgat gaaaaatgac ccggacatgg
    11221 tcgacgcggg aaaaagactc agaggttact ttgctgacca aaaaagctgg gaagaagttc
    11281 aaccttatct gaacgagtgt gtttttgcct taaacagaac tacaaaacct gcgtcagcgg
    11341 gcactgacta tatgtaatga ccaacaacaa aagcgataga gtggcattta aaaatcactc
    11401 tatcgcttaa cttattcttg agcgctgcca tcaatcaatg ccgttatata tttgtgcgaa
    11461 tctatcattc tattgaaaac ccatatcatc ccggcaaagc cacccaccac tgccagcaaa
    11521 aaacccaaaa cacctttaat cgcgactcca aatccgacgc acattgctga aaagcagaaa
    11581 cccatgtagg cctttattag cactttaacc agatcaagcc aagcaccata attatcttcg
    11641 gtctgcggac actcaatcat caaagtaatc cgatgatatg ccaagccacc accaaacatc
    11701 atcattacaa atatgaaata attcattaca agactgctat ctttcatatt aagcggcaag
    11761 ctcattatat aaatagcaag agatacagct actgaccaaa agataatcac ccctcgcttt
    11821 gttacaccat gctctatttt aatgccaaaa accattctct ctttcattac acctctcctt
    11881 ttgttgagaa cagattccct tcatcgagat acagcagcgc ttttacacac cttatcgacg
    11941 aataaagaat tgacagtata ttttttagct tcaacccatc ggactaaaac tgctcgacgc
    12001 agaacaatag cgctgcttca tacgatcact atgttccttg cgatcattgt cgtaacgcgc
    12061 ccgccatgct ctgcactctt cgtgataatg ctgtttggcg gctttgcggc attcgcggta
    12121 gtcgattgag cctttgcggt ggttggcgca tacactgctg ctgttgatgt agttattaac
    12181 agctacccac tcggctaggt agttcgtgcc accattccag cttttgatcc atttcgcggt
    12241 tcgttcttgg cttacctggc gcgactttcg ctcactgaca gttggtgcgg aggctatctg
    12301 tgttgcgggt ggtgtgtagg tgttagccgg ctgcttgggc ctgtagttat tgtcgttaaa
    12361 gctgttcttc gcacgctctg tcgcaatggc ttccatgcgc tccacccact caatctcagc
    12421 cttacttgct ggcggctgaa caaccggctg agcaggttct agtgacaaag atggtaaacg
    12481 gattggcgca gcaggttcgc tgtatgctgg ggcctgggct tgaactggca gttcgctgaa
    12541 tagaggctga ccattaacgc gaatggcctg tttaagctgg ttaacgtcga tgacgattgg
    12601 cttggcaaat agcgcgatca gcccccatgt gatcgctgag ccaacgccca ggatgacgac
    12661 cgtacgccat ggggattgct tattattgtt gcgtatataa tccggcgcat catcccagtc
    12721 gcttttcatg ttgcttcctt accgttaacc tagttaacaa atattgcctt cacccgctac
    12781 aatagaacta tttacccctt attctctcga agtcctcgtg agaataattt ttcttaaaga
    12841 actctctatc tattgtcttt ccagcctcta tctcatgaaa catcttatga tcgaccaagt
    12901 ctaaatgctt taaatactca gcatgggcat cgctcatggg gacaacctca gggtttttaa
    12961 gattatctag atgatgtgaa atgctccaga ccaaactccc acaacctcga taacccgtct
    13021 ttattaagcc cccccaataa tcaaaccttt ccttgcttaa actttcaact ccttcgatag
    13081 aaagcatctc ttggatcttt cttgtctcct gaaggtacgc atcaatatct tcattcaccg
    13141 cttcaagagc tgcgtgtatt atcttaaatg attttctttc acttttgctc aggcctaaaa
    13201 ctgcatcctt gtaataattt ttaaatataa aactggaaat acttgcaggc ccttcattac
    13261 tcacaccgcc ttgaccatat atatgcagct gccttgtata cgcatcaata gtacctttaa
    13321 tgtcatcatg caactcttcc agctccataa tcagaagctt tctaagtctc ttcctatata
    13381 aataatcttt tgccatcgct gtaaattgag caagaaccca ccctaccaat gctcctgata
    13441 cagcaatcac tactgccatt agttccatag ctgatccttt actatcttct tgatgatcct
    13501 aaccattttc tttggcatac catcgcctag ccacttccgt tgtaatcgct atccctcgtt
    13561 ttgattgggc aagtttgaat cagcttcttc gtatcctggc gatacttgac cttttgtagg
    13621 atctacatcc cctttccaaa gccatagctc atattggggg ggaagctcac aacacctctt
    13681 ccaaatcctc aatacgggtc ttgaccttcc gattggctgc cttggtctac accgcaggcc
    13741 tccttgaccg ttgtagctgc agcgagcctt gtggcaccga tgtaccgcac aagggtttta
    13801 agcctttcct cgaacaatca aaagcgctta aacacgatga ccaagcctcg taaatccaca
    13861 cggatagtga ccgaacaaac ctggaactgg aagagctgga accttcgacg ctggcaggcc
    13921 cgctacagga cgttgaaacc atctaatcac ggtcgtagcg caacggcctt ggctatggcg
    13981 catcccacat ctgcgccata aaggcgactg cggaagatgg ataagcgcat gcctccctgg
    14041 gtgattgctt agccttccac caaccagatt tcaccccagc tcacggcctt tgatcgcaag
    14101 catctgaaag tatgtgaaaa atttcaaacc aggggttcgc caaacgcact tcagctgtta
    14161 tcatgcgcgc cgccacggga agatgccgga gtggccgaac gggacggatt cgaaatccgt
    14221 tgtgttagcg atagcaccta gggttcaaat ccctatcttc ccgccatatc tcaagctgaa
    14281 gcccctgaaa ctcctagagt ttcgggggct tttgctatct gcggcatgca cggtgtcgaa
    14341 gaagtgtcga aagctggttg aggctgcagg ttagccctgg agcgggggcc acattctggc
    14401 gacgtgcacc agggcgagaa tccagacagc gtcagtctca gtctgataaa cgagccggta
    14461 gttttcgtgg ggaatcagct cacgagtacc ggcaaccgtt cccagtctcc ccttttgggg
    14521 atagtcagcg agggtggatg cagccgcact gaacagttca tccatgtgca cggcagcgcg
    14581 agtgctgtcg gcggcgatgt attcccagat atccagacga tcctgcaggg cctcgggcgt
    14641 ccaaataacc ttcattcgtg gccttgagcc ttagcacggc gagcggcgaa ttcagcctct
    14701 acatcatcgt tggatcgacc acggccagcc tctaccgagg ccctggcaac agcaaccttg
    14761 cgttgcacga actcatcatg ctctttggcc tgttgctgct ggcggatgaa atcgcgcata
    14821 aggtcacgca tgacctgaga ggccggcagg tgtgcggctt gggcggcggc cataaattct
    14881 tcgcgtagct cgggttccaa cttcaaattg aaaacggctt ctttggccat ggtgcgtcac
    14941 ctctaagtaa atccgtactt acaacgtata tacgttgtat gcatgctaaa caatgcacgc
    15001 cgcataggaa agcacagggc atcggctaca gctgaggtgt gatgatgctt agaaagcgct
    15061 gatgatcgct ggctgggccg aatttgccgg catcctgcag gtgatcgagt gcaaggtgtg
    15121 catagcgcat ggtcaaggtg gtatgcccaa ggatcttctg caaggtcaaa atgctcccgc
    15181 cattcatgac gaaatggctg acgaatatgt atcgcagtac gtgcgaggac tctggccagc
    15241 aggaatggat ctggggcttt tgcgtttcag gtcggtcaat aaatgccgaa agcgcggtgc
    15301 aggctaaacc ccacaaaaaa ggggcgccgt agcgccccat ctgtgtcgcg gtcacccgcg
    15361 cagtgctgcg gcttagacgg tcagacgcgt cagggcttcg cggtatttgt cggcggtttt
    15421 ctgcgcgacg tcggcgggca ctgctggggc gggtggctct ttgttccagc cggtggactc
    15481 cagccagtca cgtacaaact gcttgtcgaa gcttggcggg tttgtgcctt ctgcatagct
    15541 gtccgccggc cagaaacggc tggagtccgg ggtcagggct tcgtccatta gggtcagggt
    15601 gccgtcttca tccaggccga attcgaactt ggtgtcggcg atgatgatgc cacgcgtggc
    15661 tgcgtattcc actgctgcgc tgtacagggc aatggaggtg tcgcgcactt tggcggccag
    15721 ctctgcgccg atgatggctt cgcactgctc gaagctgatg ttctcgtcgt ggtcgcccac
    15781 ggcagccttg gtcgaggggg tgaagattgg ctgcggcagc ttggccgctt ccttgaggcc
    15841 tgctggcagt tgaatgccgc agacggtgcc gctcttctgg tattccttcc agccggagcc
    15901 aacgatatag ccacgtacga tggcttctac cgcgaccggc ttaaggcgct tggcgactac
    15961 tgcgcgacct tccaccagcg gtagttcggc ggccgggacg atgtcttcga ccttgtcgcc
    16021 ggtgaagtgg ttgggcacca ggtccttgag cttgtcgaac cagaagttgg agatggcggt
    16081 gaggatcttg cccttttccg ggatcggctc gttgaggatc acgtcgaatg ccgaaaggcg
    16141 gtcggtggcg accatcagca tgcgtttgtc gtcgatctcg tatagatcac ggactttgcc
    16201 cgaatagatt ttcttcaggc tcagggtggt ggctgtgctc atgtcggaat ttccgccttt
    16261 ggtaaaaaaa acaggcgaaa cccgccgggt ttcgcctatt ggtagtgccg gtgcagcagt
    16321 tgcctgctgt cagccaaggt tttcctggat caagtccagc accttgcgcg ccacgtcggc
    16381 gggggcaatg gtgttgaggt ctttttccac ggtgacctgc acgctgtcgc caaccgcagt
    16441 caggcgtacc tgatagcgct cggcgcgggc gtcgatctct tccttgctcg gctcgctgcc
    16501 aaatacgcta ccgaagaagc caggcttttc atccggcttc tgcgcacgtt cggcgaggtt
    16561 gatgtagtac acgcccaggc tgcggttgat gtcgtcgatg cgtacatccg ccatttccag
    16621 ggagcggccc acaccggacc aggcgcggtc gaaatcagca cccaggctca gcaccgggtt
    16681 gccgttaccg tcttcagaca ggctgacgcg attcggcgca tcgtaatcac gggcggccaa
    16741 cagagagacc gaaccgccct gctctgcgct gcgcgccagg gtcaccagca tttcatcgag
    16801 cagcgcagca tccaggcttg ggttgctgct gcggttggtg aaagcgacat cggcagtgct
    16861 gccaactgga cgctcggcgc tgaccacgaa gatttcgctg gtattacgct gcacgcctgg
    16921 ctcgatacgc acccgcacgc gggtttcggc atctggattg acgccagata cgcggctgct
    16981 caggcgacgc gccatggatt cggacagggc atcaaaacgc tgccatgtgg tgctgaattc
    17041 gcccgtttgc ggacgctctt cagcaatctg aaaaccgttg tcggcaaaga actgacgcgc
    17101 cacaggccag acttcagccg gtacgcgctg agctacgacc caacgcgact cgccgctctt
    17161 ttgcaggctg aactcgctgg catcaccggc cacagccagg gcttgcgggc gcggcacgat
    17221 aaattcgccg gtatcggtgg tagtcgcgac ctgctgcggt acgggcagca gcgggtcgat
    17281 acgcttggcg tcgacgttag gcgggatttg catcgtggcc gtctgacggg cttcgaggta
    17341 gtcgctgcca cggtcacgga agtagccgtt ttcgccatag atccagccgc aaccgctggt
    17401 accagcgatg atcagggcca gggcggagag tccggtgagt cgcttcatgc gtagtaattc
    17461 ctcaattaaa ccaatacgcc ggactggcgc agggcctgac gcagcggttc atgacaacgt
    17521 gggctgagcc aggtcagtgg cagacggata ccgtctggca
//
LOCUS       DASRUM010000068         5589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1711294, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000068 DASRUM010000000
VERSION     DASRUM010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711294"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..715
                     /locus_tag="VFF22_04805"
     CDS             77..715
                     /locus_tag="VFF22_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZX16173.1"
                     /translation="MHKEPRKVREFRRREQEILDTALKLFLDQGEDSVTVEMIADTVG
                     IGKGTIYKHFKSKAEIYLRLMLDYERDLNELLHSADIDRDKESLSRAYFEFRMRDPQR
                     YRLFDRLEEKVVKGNQVPEMVEQLHKIRASNFERLTQLIKGRIAEGKLEDVPPYFHYC
                     AAWALVHGAVALYHSPFWSNVLEDQEGFFQFLMDIGVRMGNKRKREGDTPAS"
     gene            795..1763
                     /locus_tag="VFF22_04810"
     CDS             795..1763
                     /locus_tag="VFF22_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010208395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZX16174.1"
                     /translation="MIVDRQGRRFRNLRISLTAACNYACTYCVPDGKRLVAAQDELSA
                     EAMLRGVAYLIEAAGIERLRITGGEPLVSPKLDAFLRGVSQLGLADISLTSNGQLLAR
                     KLPLLLECGIRRLNISLDTLDAAAFRTIARGGDLATVLDGLQQARDAGIQIKVNMVPL
                     RGQNLDQVLPLLDYCLANGFELRFIELMRMGHLARDGNGFQRQFVGMPELLELIGSRY
                     DYIQAHAPLDATALRFAIPGVGHFGVIANESVPFCRTCSRLRLSSTGWLHGCLSSSNR
                     HYIGDLLVKPRHQALPALQGLLVKALGDKQDVAFSGGATVMKIIGG"
     gene            1791..2681
                     /locus_tag="VFF22_04815"
     CDS             1791..2681
                     /locus_tag="VFF22_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009621455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZX16175.1"
                     /translation="MQDFPISYIEPVFRPPSEAHSLILPVTNGCSWNKCTFCEMYTQE
                     QKKFRARDEAQVLEEIRLTGERMIVQRVFLADGDALVLPTRRLLTILQAIREHMPEVE
                     RVSSYCLPRNLRKKSVDELKELADAGLRMAYVGCESGDDEVLARVNKGETYESSLSAL
                     DKLGQAGITRSVMILNGLGGSVLSEQHADNSARLMNEAQPEFLSTLVVSFPTGEARFR
                     EGFADFQPLDQQQLFVEVERLLQGLELRDTVFRSDHASNYLVLKGTLGADKQRLLAQV
                     RQAIEQPQQAQLRQEWQRGL"
     gene            2758..3360
                     /locus_tag="VFF22_04820"
     CDS             2758..3360
                     /locus_tag="VFF22_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4823 domain-containing protein"
                     /protein_id="HZX16176.1"
                     /translation="MRSLLLIVGMVLLSGCMNASDLADEAIYQLRDVGVLDHSQTRRA
                     SSWRLQADSFIYIAQGHFVPPGSAYPRPNVVAEEAYKGFIEYFPMVRRAKAPLGLEES
                     LAAAREAGAHYLLYSRFAYADDRIGTVEEWEDQEALDRLGTDRGVIQLMLIETNTRYL
                     VDTTRIRSRGGFLTLYDAKPEDLIGPALQDYARRLLGLGR"
     gene            3364..3933
                     /locus_tag="VFF22_04825"
     CDS             3364..3933
                     /locus_tag="VFF22_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HZX16177.1"
                     /translation="MTDTGKAGDLLAQIPKGEGKGLPPVHLWHPTFCGDIDMRIARDG
                     TWYYLGTPIGRKPMVKLFSTIIRRDGDKYVLVTPVEMVGIQVDDAPFVAVSLQVEGEG
                     EAQVLRFVTNVEDEVVAGGEHPLRVELDALTDEPAPYVHVRSNLEALIHRNVFYQLVE
                     LAVPREIDGRTWLGVWSGGEFFPIGPQPD"
     gene            complement(3992..>5589)
                     /locus_tag="VFF22_04830"
     CDS             complement(3992..>5589)
                     /locus_tag="VFF22_04830"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HZX16178.1"
                     /translation="CRLKQKAAEAGKEISVCVVEKGSEVGAHILSGAVFEPRALNELF
                     PDWKELGAPLNTPVKRDDIYVLSNAEKATKVPDFFVPKTMHNHGNYIISLGNLCRWLA
                     QQAENLGVEIYPGFAAQEALINEEGAVYGILTGDLGVDREGHPKEGYYTPGMELRAKY
                     TLFAEGCRGHIGKQLIKKFNLDSEADAQHYGIGIKEIWDIDPAKHEQGLVVHTAGWPL
                     DIVASENTGGSFLYHLENNQVVVGLIVDLSYSNPHLSPFDEFQRYKHHPVIAQYLEGG
                     KRVSYGARAICKGGLNSLPKMVFNGGALIGCDLGTLNFAKIKGSHTAMKSGMLAADAV
                     ADALFAGKEGGDQLTGYVDAFKASWLYDELFRSRNFGPALHKFGPLVGGGFNWLDQNI
                     FGGKIPFTLHDTKPDYACLKPAADAQKISYPKPDGKLSFDKLSSVFLSNTNHEEEQPC
                     HLKLTDPSIPLSKNLPMYDEPAQRYCPAGVYEIVTKEDGEQKFQINAQNCVHCKTCDI
                     KDPAQNINWVAPEGTGGPNYPNM"
BASE COUNT         1002 a         1552 c         1799 g         1236 t
ORIGIN      
        1 ggtcggatga tccgtgcaat ttgccgttag ttaggcataa tacgcgcctt cgtttttttg
       61 accccgtagt ccttttatgc ataaagaacc ccgcaaggtc cgtgagtttc gccgccgcga
      121 acaggaaatt ctcgacacag ccctgaaact cttcctcgac cagggtgaag acagcgtgac
      181 tgtggagatg attgccgaca cggttggtat tggcaaaggc accatctaca aacacttcaa
      241 atccaaggcg gagatctacc tgcgcctgat gctcgactat gagcgtgatc tcaacgagct
      301 gctgcactcg gccgatatcg accgcgacaa ggaatccctg tcgcgcgcct acttcgagtt
      361 ccgcatgcgc gacccgcagc gctaccgcct gtttgaccgc ctggaagaga aggtggtcaa
      421 gggcaatcaa gtgccggaga tggtcgagca gctgcacaag atccgcgcct ccaacttcga
      481 gcgtctgacc cagctgatca agggccgcat cgccgaaggc aagctggagg acgtgccgcc
      541 gtacttccat tactgtgccg cctgggcgtt ggtgcacggc gctgtggcgc tgtatcactc
      601 gccattctgg agcaacgtgt tggaagatca ggaaggtttc ttccagttcc tcatggatat
      661 cggtgtgcgc atgggcaaca agcgtaagcg tgagggcgac accccggcta gctgaggtat
      721 tcagcgggtg gataacgaat ctgctgcgtt ggcggcgccc ctatactcgg tgcttacctt
      781 tgctggagtc atgcatgatc gttgaccgcc agggcaggcg ttttcgcaat ctgcggatca
      841 gcctgaccgc cgcctgcaac tatgcctgca cctactgcgt acccgatggt aagcggctgg
      901 tagcagcgca ggacgagctg tcggccgagg ccatgctgcg tggcgtggcc tatctgatcg
      961 aagccgccgg tattgagcgc ttgcgcatca ccggtggcga gccgctggtc agccctaaac
     1021 tcgacgcttt tctgcgcggc gtcagccagc tcggcttggc cgatatcagc ttgaccagca
     1081 acggtcagct gctggcgcgc aagctaccgc tgctgctgga atgcggcatt cgccgcttga
     1141 atatttcctt ggacaccctc gacgctgcgg cattccgcac cattgcccgt ggcggcgatc
     1201 tggcgacagt gctcgatggc ctgcagcagg cgcgggatgc ggggatacag atcaaggtca
     1261 atatggtgcc gttgcgcggg cagaacctcg atcaggtgtt gccgctgctc gactattgcc
     1321 tggccaatgg cttcgagctg cgctttatcg agctgatgcg catggggcat ctggcccgcg
     1381 acggcaacgg cttccagcgc cagtttgtcg gcatgcccga gctgctggaa ctgatcggca
     1441 gccgctatga ctatattcag gctcacgcgc cgttggatgc gaccgcgctg cgtttcgcca
     1501 ttcccggcgt gggccacttc ggcgtaatcg ccaatgaaag cgtgccgttc tgccgcacct
     1561 gctcgcggct gcgtctgtcc tcgaccggct ggttgcatgg ttgcctgtca tcgagcaatc
     1621 gccactacat cggcgacctg ctcgtcaagc cgcgccatca ggccttacct gcgctgcagg
     1681 gcctgttggt caaggcgctg ggcgacaagc aggatgtggc gttctccggc ggcgcgaccg
     1741 tgatgaagat tattggcggc taagaccgcc gcctgccttg agtgaagacc atgcaagatt
     1801 tccccatcag ctatatcgaa ccggtatttc gtccgcccag cgaagctcac tcgctgattc
     1861 tgccggtgac taatggttgc tcttggaaca agtgcacgtt ctgtgaaatg tacactcagg
     1921 agcagaagaa attccgcgcc cgcgatgagg cgcaggtgct cgaagagatt cgcctgaccg
     1981 gtgaacggat gatcgtgcag cgggtgtttc ttgccgatgg tgatgccctg gtgctaccga
     2041 cacggcgttt gttgaccatc ctgcaggcga ttcgtgaaca catgccggaa gtcgagcgag
     2101 tgtcgagtta ttgcctgccg cgcaatctgc gcaagaagtc cgtagatgag ctcaaggagc
     2161 tggccgatgc tgggctgcgc atggcctatg tcggctgcga gtcgggtgat gacgaggtgc
     2221 tggcgcgggt caacaagggc gagacctatg agtccagcct gagtgcgctg gacaagcttg
     2281 ggcaggccgg catcacccgc tcggtgatga tcctcaacgg tctgggcggg tcggtcttga
     2341 gtgagcagca tgcggacaac tcggcgcgcc tgatgaatga ggcgcagccg gagtttctct
     2401 cgaccttggt ggtaagtttc cccacgggcg aggcacggtt tcgcgaaggg tttgccgact
     2461 tccaaccgct cgatcaacag caactgtttg ttgaggtgga gcgtctgctg caggggcttg
     2521 agttgcgtga cacggtattt cgcagtgacc atgcctccaa ctatctagtg ctcaagggca
     2581 ctcttggcgc tgacaaacag cgcctgctgg cacaggtgcg gcaggccatc gagcagcccc
     2641 aacaggcgca gttacgtcag gagtggcagc gcgggctttg atgcgtagca ggtggcgtga
     2701 aatctgcatc taggcgctat ccgccggttt tttgtcaccg gctgcgggag aagcgtgatg
     2761 cgtagtctgt tgctgatagt aggtatggtg ctgctgagcg gttgcatgaa tgccagtgat
     2821 ctggcggatg aagcgattta tcaattgcgc gatgtcggcg tgctcgacca cagccagacg
     2881 cggcgcgcca gttcgtggcg cttgcaggcc gattcgttca tatatatcgc ccaaggtcat
     2941 ttcgtgccgc cgggctcagc ctatccgcgg cccaatgtgg tggccgaaga ggcctacaaa
     3001 ggctttatcg agtatttccc catggtccgt cgcgccaagg cgccgctagg gttggaggag
     3061 tctcttgctg cggctcgtga agccggggcg cattacctgc tgtacagccg ctttgcctat
     3121 gccgatgacc gtatcggcac ggtggaggag tgggaagatc aggaggcgct ggatcgccta
     3181 ggcactgatc gcggggtgat tcagttgatg ctgatcgaga ccaatacccg ctatttggtc
     3241 gataccacgc gcattcgtag ccgcggaggc ttcctgacgc tttatgatgc caagcctgag
     3301 gaccttatcg gtccggcgct gcaggattac gcgcggcgtt tattgggctt ggggcggtga
     3361 ttgatgactg atacaggcaa ggccggcgac ctgctggcgc agatacccaa aggcgaaggc
     3421 aaagggttgc cgccggtgca tctgtggcac ccgacgttct gcggtgatat cgacatgcgt
     3481 atcgcccgcg acggcacctg gtattacctg ggtacgccga tcgggcgcaa gccgatggtc
     3541 aaactgttct ccaccatcat tcgccgtgat ggcgataaat acgtgctggt gacaccggtg
     3601 gagatggtcg gcatccaggt tgatgacgcg ccctttgtgg cggtcagtct gcaggtggag
     3661 ggtgagggcg aggcgcaggt gctgcgcttt gtcaccaacg tcgaggatga ggtggtggcc
     3721 ggcggcgagc atccgctgcg ggttgagctg gacgccctga ccgatgagcc cgcgccctat
     3781 gtgcatgtgc ggagcaatct cgaggcgctg atccatcgca atgtgttcta tcagctggtc
     3841 gagttggcgg tgccgcgcga gatcgatggc cggacctggc tcggtgtgtg gagcggtggc
     3901 gagttcttcc cgattgggcc gcagccggac tagcactggc ggctgtattt ggtgcaataa
     3961 aaaaggctcc cgagggagcc ttttttgcgt cttacatatt ggggtagtta ggcccgccgg
     4021 tgccttcagg ggccacccag ttgatgttct gggcggggtc cttgatgtca caggttttgc
     4081 aatgtacgca gttctgcgcg ttgatctgga acttctgctc gccatcttcc ttggtgacga
     4141 tttcgtaaac gccggccggg cagtagcgct gcgccggttc gtcgtacatc ggcaggttct
     4201 tgctcagcgg aatgctcgga tcggtgagct tcaggtggca gggttgttcc tcttcatggt
     4261 tggtgttgga gaggaatacc gagctgagct tgtcgaagct gagtttgccg tcgggcttcg
     4321 ggtagctgat cttctgcgcg tctgctgccg gcttcaggca ggcgtaatcc ggcttggtgt
     4381 cgtgcagggt gaaggggatc ttgccgccga agatgttctg gtcgagccag ttgaagccgc
     4441 cgccaaccag cgggccgaac ttgtgcagcg ctgggccgaa gttgcggctg cggaacagct
     4501 cgtcgtacag ccagctggcc ttgaatgcgt cgacgtagcc ggtcagctgg tcgccgcctt
     4561 ccttgcctgc aaacagcgcg tcagccacgg cgtcagcagc gagcatgccg gacttcatgg
     4621 cggtgtggct gcccttgatc ttggcgaagt tcagggtgcc gaggtcgcaa ccgatcagtg
     4681 caccgccgtt gaagaccatc ttcggcagcg agttgaggcc gcctttgcag atggcgcggg
     4741 caccgtaaga aacgcgcttg ccgccttcca ggtactgggc gatcaccggg tggtgcttgt
     4801 agcgctggaa ctcatcgaac ggcgacagat gcgggttgct gtacgacagg tcgacgatca
     4861 gaccgactac cacctggttg ttttccaggt gatagaggaa ggagccgccg gtgttttcgc
     4921 tagcaacgat atccagtggc cagccggcgg tgtgtaccac caggccttgc tcgtgcttgg
     4981 cggggtcgat gtcccagatt tccttgatgc cgatgccgta atgctgagcg tcggcttcgg
     5041 agtcgaggtt gaatttcttg atcagctgct tgccgatatg gccacggcag ccttcggcga
     5101 acagcgtgta cttggcgcgc agttccatgc ctggggtgta gtaaccctct ttcgggtgac
     5161 cttcgcggtc gacacccaga tcgccagtga gaatgccata taccgcgcct tcttcattga
     5221 tcagcgcttc ctgagcggcg aagcctgggt agatttctac gccgaggttc tctgcctgct
     5281 gcgccagcca gcggcacagg ttgcccaggg aaataatgta gttgccatgg ttgtgcatgg
     5341 tcttgggcac gaagaagtcc ggaaccttgg tggccttctc ggcattgctg agcacgtaga
     5401 tgtcatcgcg cttgactgga gtattcagcg gtgcgccaag ctctttccag tccgggaaca
     5461 gttcgttcag ggcacgaggc tcgaagactg cgccagagag aatgtgagcg ccaacttcgg
     5521 agcctttctc taccacgcaa acgctgattt ctttgccagc ctcagcggcc ttctgtttca
     5581 gtcggcagg
//
LOCUS       DASRUM010000069         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_37320, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000069 DASRUM010000000
VERSION     DASRUM010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37320"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..985
                     /gene="trxB"
                     /locus_tag="VFF22_04835"
     CDS             35..985
                     /gene="trxB"
                     /locus_tag="VFF22_04835"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HZX16179.1"
                     /translation="MSEVKHSRLIILGSGPAGYSAAVYAARANLKPVIITGIQPGGQL
                     TTTTEVDNWPGDVEGLTGPALMERMQKHAERFETEIVYDHIHTAELQSRPFTLKGDSG
                     TYTCDALIIATGASAQYLGLPSEEAFSGKGVSACATCDGFFYRNQVVAVIGGGNTAVE
                     EALYLSNIAKEVHLVHRRDKLRSEKILQDKLFDKAANGNVVLHWNHSLEEVLGDNTGV
                     TGARLKDTQSGVSKDLPLAGVFIAIGHKPNTELFHGQLEMRDGYLLIKGGNEGNATAT
                     SIEGVFAAGDVADHVYRQAITSAGAGCMAALDVEKFLDDN"
     gene            1046..1726
                     /gene="aat"
                     /locus_tag="VFF22_04840"
     CDS             1046..1726
                     /gene="aat"
                     /locus_tag="VFF22_04840"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020191944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HZX16180.1"
                     /translation="MLTWLQRDSLDFPPLNKALREPNGLLAAGGDLRPERLIQAYRHG
                     CFPWFQDGQPILWWSPDPRTVLLPDKLHISRSLAKVLRQGRYRVTFDQAFADIIRACA
                     APRSYAAETWITSPMQDAYLELHRHGIAHSVEVWRDSELVGGLYGLAMGQLFFGESMF
                     SRADNASKVGFVTLVEHLKDWGFVLIDCQMPTEHLLSLGAQTISRSEFAGYLNANLDQ
                     PSLADWVA"
     gene            1782..2489
                     /locus_tag="VFF22_04845"
     CDS             1782..2489
                     /locus_tag="VFF22_04845"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011334947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HZX16181.1"
                     /translation="MTELARLKFYATQPHPCSYLPDEQATTLFLDPSQPMDADVYAEL
                     SEMGFRRSGDHLYRPHCQRCTACVPARIPAAQFSPNRQQRRIFKRNQDIQVRLVRPAF
                     TEEYYALYVRYIEQRHADGDMYPPNREQFSTFLVRDLAFSRFYEFRQDSRLLAIAVTD
                     VLPNGLSAVYTFYEPSEERRSLGRFAILWQIAEAERLGLHAVYLGYWIKNCRKMNYKT
                     QYRPIELFINQRWVSLN"
BASE COUNT          527 a          795 c          726 g          555 t
ORIGIN      
        1 tctcttttgt gacaaaggct tatgaggtgt ttttatgagc gaagtcaagc attcacgctt
       61 gatcatcctg ggctccggcc ctgccggtta tagcgctgcc gtttacgctg cccgcgccaa
      121 cctcaagcct gtgatcatta ccggtattca gcccggcggt cagctgacca ccaccaccga
      181 agtggacaac tggccgggcg atgttgaagg cctgaccggc ccagcgctga tggaacgtat
      241 gcaaaagcat gccgagcgct ttgaaaccga gatcgtctac gaccacatcc acaccgccga
      301 gttgcagtcg cgccctttca ccttgaaagg cgacagcggc acttacacct gtgatgccct
      361 gattatcgcc acgggcgcat cggcgcagta cctgggcctg ccgtccgaag aagcgttttc
      421 cggcaagggc gtatcggcct gcgccacctg cgatggcttc ttctatcgca accaggtggt
      481 cgcggtgatt ggcggcggca ataccgcggt cgaagaagcg ctgtacctgt cgaatatcgc
      541 caaggaagtg cacctggtgc atcgccgcga caagcttcgt tccgagaaga ttcttcagga
      601 caagctgttc gataaagcag ccaacggcaa cgtggtgctg cactggaatc actctcttga
      661 agaagtactg ggtgacaaca ccggcgttac cggtgcccgc ctgaaagata cgcaaagtgg
      721 cgtgagcaaa gatctgcctc tagccggcgt atttatcgcc atcggccata aacccaatac
      781 cgaactgttc cacggccagc tggaaatgcg cgatggctac ctgctgatca agggtggcaa
      841 tgaaggcaat gccaccgcca ccagcattga aggtgtgttt gctgcgggcg acgtggctga
      901 tcacgtttac cgccaggcca tcacctcggc aggcgcaggc tgcatggccg cgctggatgt
      961 cgagaaattc ctcgacgaca attgatccat tcagggcgga cgcacagtcc gccctgccct
     1021 cctccgtcca actccggccc tgcacatgct gacgtggctg caacgcgact cactggattt
     1081 cccgcctctt aataaagccc tgcgcgagcc gaacggcctg ctggcggcgg gtggcgatct
     1141 acgccccgaa cgcttgatcc aggcttatcg gcacggttgc ttcccctggt ttcaggatgg
     1201 ccagccgatt ctctggtggt cgccggaccc gcgcaccgtg ctgttgcctg acaagctgca
     1261 tatctcgcgc agtcttgcca aggtgctgcg tcagggccgt tatcgggtga cattcgacca
     1321 ggctttcgcc gacatcatcc gcgcctgcgc cgccccacgc agctatgcgg cagaaacctg
     1381 gatcaccagc cctatgcagg atgcctacct ggagctgcac aggcacggta ttgctcattc
     1441 ggttgaggtt tggcgcgata gcgagctggt tggtgggctc tatggcctgg ccatgggcca
     1501 gctgttcttc ggtgagtcga tgttcagccg cgcggacaat gcatccaagg tgggttttgt
     1561 cacgctggtc gaacacttga aagactgggg ctttgtgttg atcgattgcc agatgccaac
     1621 tgaacaccta ctcagcctcg gcgcgcagac gatttcccga agcgagttcg ccggctatct
     1681 gaatgcaaat cttgatcaac ccagcctggc cgactgggtc gcctaggcga gtttgccagc
     1741 ctggcttaca cttgattcaa gaccgatcct gagagtcgat catgactgag ctggcccgcc
     1801 tgaagtttta cgccactcaa ccgcaccctt gcagctatct gcccgatgag caggccacaa
     1861 ccctgttcct cgaccccagc cagcccatgg atgcggatgt ttacgctgag ctttcggaaa
     1921 tgggctttcg ccgcagcggc gaccacctct atcgcccaca ttgtcagcgc tgcacggctt
     1981 gcgtcccggc gcgcattcct gccgcgcagt tcagcccgaa tcgccagcaa cggcgcatct
     2041 ttaagcgcaa ccaggacatt caggtacgcc tggtgcgccc ggcatttacc gaagagtatt
     2101 acgcgttgta cgtacgctac attgagcagc ggcatgccga tggcgatatg tacccaccca
     2161 accgcgaaca attttcaacc tttctggtgc gtgatctagc cttctcgcgc ttctatgagt
     2221 tccgccagga cagccgcctg ctggcaattg ccgtaaccga tgtgctgccc aatggcctgt
     2281 ctgcggttta cactttttac gagccgagcg aggaacggcg cagcctcggg cgctttgcca
     2341 tcctctggca aattgccgaa gcggagcgat tggggctgca tgcggtctat ctgggttact
     2401 ggatcaagaa ctgccgcaag atgaattaca agacccagta ccgaccaata gagctgttca
     2461 taaatcaacg ctgggtcagt cttaactgaa gcccttggcg caaaccccaa atttcgggca
     2521 caatgcacgc cgcttttgcc tggcgccagt tgcaccgggc cattcattgg ataccgaggg
     2581 ctttactgca tgtcgaaaga aga
//
LOCUS       DASRUM010000070        25127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1712153, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000070 DASRUM010000000
VERSION     DASRUM010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 25127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25127
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712153"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VFF22_04850"
     CDS             <1..880
                     /locus_tag="VFF22_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003198456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HZX16182.1"
                     /translation="PGAPVIEASSGSTAISEAYFARLLGLPFIAVMPASTSKEKIAQI
                     AFYGGQSHLVTDPTQIYAESERLARETGGHFMDQFTYAERATDWRANNNIAESIFQQL
                     RSERFPEPSWLVSSPGTGGTLATLGRYVRYRRHSTQVLCADAERSVFFDYYRTGDVSL
                     CLNHGSRIEGIGRPRVEASFLPSVIDAMCKVPDALSLAAMHYLAQRLGRKVGGSSGTN
                     LIGALLVAQRMVAAGESGSVVAILCDGGERYADTYYDQAWLSAQGFDLAPLLEAVAAC
                     AERGVSLPGDLPTASL"
     gene            978..1487
                     /locus_tag="VFF22_04855"
     CDS             978..1487
                     /locus_tag="VFF22_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX16183.1"
                     /translation="MSDFEIRQAQELDIDNLCALILEHGPNPWNYLPPDEVAAHLQGI
                     ADGAVQAVLAERAGELQGFVSYQLSRHFERYQPEARRGQPHGYICEAVTHRAVAGQGL
                     GSRLLQHAVMQLREQDVSDIYIDRHEENAASAGMMRKAGFVEIETYADPQRRPNGSGR
                     TTLCCLRSA"
     gene            1530..1925
                     /locus_tag="VFF22_04860"
     CDS             1530..1925
                     /locus_tag="VFF22_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HZX16184.1"
                     /translation="MIQCKRVYEASSAADGRRVLVDRLWPRGLSKAKLQLDAWLPEVA
                     PSTQLRRQFAHEPEAFEAFRAQYRVELAAHPEYWQGLLEPAEQGVLTLLFSARDTQLN
                     NAQVLAEFLEDELERRGPSSSPVCYTDRM"
     gene            complement(1934..3550)
                     /locus_tag="VFF22_04865"
     CDS             complement(1934..3550)
                     /locus_tag="VFF22_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZX16185.1"
                     /translation="MLRITELKLPLDHPDEALRPAIIQRLGIADSELLDFSLFKRSYD
                     ARKKSTEMHFIYTIDCELRDEAALLATFSDDRHISVTPDTAYKPVGMAPENLAERPLV
                     VGFGPCGIFAALILAQAGLKPIVLERGTEVRQRTKDTWGLWRKNVLNPESNVQFGEGG
                     AGTFSDGKLYSQIKDPKHYGRKVLHEFVKAGAPDEILYVSKPHIGTFRLTGVVATMRE
                     EIKALGGEVRFQQRVSDVLIEDGQLKGVVLDSGEQILSRQVILALGHSSRDTFRMLHK
                     REVFMEAKPFSVGFRIEHPQGLIDRARLGKYAGHPKLGAADYKLVHHAKNGRSVYSFC
                     MCPGGTVVAATSEPNRVVTNGMSQYSRNERNANAGIVVGITPEQDYPGSPLAGVELQE
                     RLESQAYLLGGSTYEAPGQLVGDFIAGKPSTELGSVEPSYKPGIKLGDLALALPDFAI
                     EAIREALPAFGKQIKGFDLHDAVLTGIETRTSSPLRITRGADMQSLNVRGLYPAGEGA
                     GYAGGILSAGVDGIRVAEALIRDMLGIAES"
     gene            complement(3751..4749)
                     /gene="gap"
                     /locus_tag="VFF22_04870"
     CDS             complement(3751..4749)
                     /gene="gap"
                     /locus_tag="VFF22_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZX16186.1"
                     /translation="MLRIAINGYGRIGRCLVRALFERKLEQQIHLTAINDLGEQSTLA
                     HLTRFDSTFGRFPGDISLDGDTLLVNGHPIQLLREREVANLPWREHAVDLVLECTGKL
                     KKRAQVEQHISAGSPRVLLSHPLDSADLTVVYGVNHQLLGDQQIVSNASCTTNCLAPM
                     AKVLHEAVGIRQGLVNTIHSYTNDQNLLDKTHSDLYRARAAALSMIPTSTGAAKAIGL
                     VMPELIGRLDGLSIRVPTPNVSLVDLTFTSERATSHEAINAALKAGALQLPPGVMECN
                     ELALVSSDFNGYPVSCVVDLSHTRVQGDLVKVLAWYDNEWAFANRMLDVMLAWVKH"
     gene            complement(4832..5503)
                     /locus_tag="VFF22_04875"
     CDS             complement(4832..5503)
                     /locus_tag="VFF22_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16187.1"
                     /translation="MRFSRPFCFALLPLLASCQVFTDKPAEPLIEQTRMQGELSLNAG
                     QLLFRPCQEQRQFILKEDSAAELSSDASTLFADGHTTLYVDMRGQLQANAQKGVDGQF
                     VPSLVYRVQAEGHGCTDINFARTILRASGHEPDWNIAVSNQGLILNRPGQEPQALPYL
                     EEQLPEGRLNLTSEANGQRLDLWVARQRCVDGMSGAVQHLSAELRLNSEVLRGCAYLG
                     GARND"
     gene            complement(5784..6041)
                     /locus_tag="VFF22_04880"
     CDS             complement(5784..6041)
                     /locus_tag="VFF22_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16188.1"
                     /translation="MFYITGRGCVVVPGIPYEFPTPVGIGAQLLIETPSGEHVRTTLI
                     SMEMISRGKPMNHAPFSVPSNIAKNQLPVGSKVFLVEDAHA"
     gene            complement(6145..7239)
                     /locus_tag="VFF22_04885"
     CDS             complement(6145..7239)
                     /locus_tag="VFF22_04885"
                     /inference="COORDINATES: protein motif:HMM:NF021640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061810 - NAD glycohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HZX16189.1"
                     /translation="MTVDDAISDLKSYVEKKLFILSNEAERVFREAESIGCKYNRDIY
                     PYVFLLAIINTFADLRGLMLRKGCDPEEAKHICTLEALTREDDKYDDNLPPYSSSEGK
                     RLGCREGFIDSAIATAIRHGRKEILKSDVIEGFLANHDQINPVLENLDWADSALHVAY
                     NTLSHILGSYNKSLWITFDDIREELNLYRENEFRKNSVDSAPQRLKSALFDLFSDWPD
                     YRKNCFLIMSFRETPFHREVHAALRKILSQHGFNLLRADDRAYSDDVLSNIETYIFGS
                     KFAFSVHDRIENENHNANVSLEIGYLMGQGKHVCLLKEQSVNSLPSDLQGRLYVSFSM
                     FNIESSLAIAVEGWLKSKRLIKSPEISAHA"
     gene            complement(7334..7777)
                     /locus_tag="VFF22_04890"
     CDS             complement(7334..7777)
                     /locus_tag="VFF22_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16190.1"
                     /translation="MASYLSDAANLATAAAVIFAAWQIWLSKKQAETTFEDSLAKEYR
                     ELAARLPTKALLGEPLTDEEYAASFDEMYHYFDLCNEQVFLKQTKRISNKTWKFWEDG
                     MKSNISRPAFERAWSEIAARSENDFSELRSIIPPKPQIQPNAVGA"
     gene            complement(7876..8370)
                     /locus_tag="VFF22_04895"
     CDS             complement(7876..8370)
                     /locus_tag="VFF22_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16191.1"
                     /translation="MAAKTKSPYLYGENRLGDVIAAIQVMGSYGYYKLDFEGWATRIS
                     GDKSKAEHWKKVFLEHPEFFRLDTAGVKASLAWRRSYRRRYSVDQQRELSLAEYQALS
                     NEDSARISRAPLNSSDISVLINTAINLHTRAIEQNREYRWLSSPLFSLLGVALGALLG
                     WLSK"
     gene            complement(8468..9097)
                     /locus_tag="VFF22_04900"
     CDS             complement(8468..9097)
                     /locus_tag="VFF22_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16192.1"
                     /translation="MDIGKIEIKLDKRWELQDLSVVTKEYVQLYSFFYVLKCVDEGIY
                     VGLDFSTYPWGGGYSVVNFFKGSYGLTPDEYRLQINRIQYASPGFIELSGVIAIASDV
                     SILVSALCASALAINKTYDTIVKSYHRRRLGHIKVQEAESKLMQDDIVFIQQSIRRLS
                     SEFNLRPEQVSAVQKITNGNELVQLKILLALYRRAEPIQEQQSSGKAQL"
     gene            complement(9186..10244)
                     /locus_tag="VFF22_04905"
     CDS             complement(9186..10244)
                     /locus_tag="VFF22_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16193.1"
                     /translation="MLSAREFYYSEIAPDEAGKNYQQAHQSAIYDEKSSKEDLSNIVG
                     HVPNLSNDILIESYIENFIASTSGTFSDSISRMFVAKRKDFLANAGAIHAGGRFEGDL
                     GFFYVGLSDLCFQYAILFGEFINLCALRQELPDYSEDVLLSMAGVVKGLNKLANAQLE
                     WGINGNEIRLKDETLIYPSDHLADNAARIATLMDRVVLGHEIAHHLLGHTGRDATTSE
                     LLEIRIAPFLNQGLSAKHRKEIEADLLGLALPLWSTFGDSSDQAEFEVALGALLFYTV
                     ISHLKCSMFLESESHPSAATRYNHVLKFIRTHFKLPVLDSLIDDIERFQALLFATQDR
                     GLGLMQKHRLSTNGERNA"
     gene            complement(10651..11205)
                     /locus_tag="VFF22_04910"
     CDS             complement(10651..11205)
                     /locus_tag="VFF22_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16194.1"
                     /translation="MDEQISKIKRTLLISSSLLLLISIASIEISEIEIPFVVTKITNT
                     YAIQFFIFIFVIYIAACFINAHKKSNPSIAKRANLYIKDNEKILEAIKKDPQFMETRS
                     RYPEVRHHFFKRNIAYQINNEGDLEERVIQIPYRTVFIPEVLAYIKYASNESSFIDYD
                     LPLLWAALSIALPLANVYLTYLSG"
     gene            complement(12014..12796)
                     /locus_tag="VFF22_04915"
     CDS             complement(12014..12796)
                     /locus_tag="VFF22_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020296183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZX16195.1"
                     /translation="MRLLYPSDPLDTKLPDESYKEEFGAALSAGISASLFSFEDFESG
                     ILKIRPEVQHGEIVLYRGWMLTPELYERLSSALHSKGVITFTSPEQYKLCHHLPEWYP
                     HLTGLTSETIVLKSDANFEKELAHLNWPGFFIKDYVKSLNTAGGSLVDTPDKIGAVVQ
                     LMQKYRGQIEGGICVRMRENYIEGTERRYFVLNGKPYAAAGNVPDIVNICARTVRSPF
                     FSVDVVLRSDNVIRVVELGDGQVSDRKEWEAERFAQMLRAAG"
     gene            complement(12880..13299)
                     /locus_tag="VFF22_04920"
     CDS             complement(12880..13299)
                     /locus_tag="VFF22_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16196.1"
                     /translation="MSPIEIILALLGLVGIGGIITNVQTRNKELAFKALESKERRYKS
                     CLLYMDVYFNPKNIKYLSSRQADIDSASDVMEYLKAEYHEMILYAAKPVVLSVKAFIE
                     QPSRENFLSAVLAMRKDLWTNKSDLTIREIAVVKSEA"
     gene            13944..14909
                     /locus_tag="VFF22_04925"
     CDS             13944..14909
                     /locus_tag="VFF22_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HZX16197.1"
                     /translation="MDGKPRLLDQVREQVCLKHYSIRTEHVYCEWVKRFIRFHRYRHP
                     EGMGAAEVEAFLSDLAVRKNVSASTQNQALAALLFLYKQVLKLDLPWLGEVVRAKKPS
                     RLPVVLSLAEVQQILAQLEGEVGLVANLLYGSGMRLMEVMRLRVKDIDFTRGEILVRD
                     GKGMKDRVTVLPRCLIERLKQHLVLVRARHNAELKEGRGDVYLPFALERKYPNGPWDW
                     RWQYVFPAAGLSVDPRSGKVRRHHLDEKRVQRAFKAAIKRTGIVKLATPHTLRHSFAT
                     HLLESGQDIRTVQELLGHADVKTTMIYTHVLNRGGLAVLSPLDRA"
     gene            complement(14960..16282)
                     /gene="rimO"
                     /locus_tag="VFF22_04930"
     CDS             complement(14960..16282)
                     /gene="rimO"
                     /locus_tag="VFF22_04930"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HZX16198.1"
                     /translation="MSTPATPKVGFVSLGCPKALVDSERILTQLRMEGYEVVSTYQDA
                     DVVVVNTCGFIDSAKAESLEVIGEAIKENGKVIVTGCMGVEEGSIRDVHPSVLSVTGP
                     QQYEQVVNAVHDVVPPRQDHNPLIDLVPPQGIKLTPRHYAYLKISEGCNHSCSFCIIP
                     SMRGKLVSRPVGEVLSEAERLVKAGVKELLVISQDTSAYGVDVKYKTDFWNGQPVKTR
                     MTEMCEALSSMGVWVRLHYVYPYPHVDELIPLMAAGKILPYLDIPFQHASPKVLKAMK
                     RPAFEDKTLARIKNWREICPELTIRSTFIVGFPGETEEDFQYLLNWLTEAQLDRVGCF
                     QYSPVEGAPANLLENPVPDDIKQDRWDRFMAHQQAISTARLQLKVGKEMDLLIDEVDD
                     QGAVARCYADAPEIDGSVFIASTNVNPGDRVRVRIVDADEYDMWAELI"
     gene            complement(16572..16648)
                     /locus_tag="VFF22_04935"
     tRNA            complement(16572..16648)
                     /locus_tag="VFF22_04935"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16612..16614),aa:Met,seq:cat)
     gene            complement(16772..16848)
                     /locus_tag="VFF22_04940"
     tRNA            complement(16772..16848)
                     /locus_tag="VFF22_04940"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16812..16814),aa:Met,seq:cat)
     gene            17055..17270
                     /locus_tag="VFF22_04945"
     CDS             17055..17270
                     /locus_tag="VFF22_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HZX16199.1"
                     /translation="MSVECKACGAQVPADAKTCPGCGVAVAAKTKLLPLAAFMLIVIL
                     IIQSYMSKSEDAASEAKPAAPVSSVEQ"
     gene            17294..18355
                     /gene="dinB"
                     /locus_tag="VFF22_04950"
     CDS             17294..18355
                     /gene="dinB"
                     /locus_tag="VFF22_04950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020289431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZX16200.1"
                     /translation="MNSRKIIHVDCDCFYAAIEMRDDPSLASKPLAVGGSADRRGVIA
                     TCNYEARSYGVRSAMASGQALKLCPDLVIVKPRIDAYKAVSREIQAIFRDYTELIEPL
                     SLDEAYLDVTDSPHFAGSATRIAQDIRRRVSQELHITVSAGVAPNKFLAKIASDWKKP
                     NGLFVITPDQVEDFVAALPVSKLHGVGKVTADKLGRLGIRTCSDLREWNKLALVKEFG
                     SFGERLWGLARGIDERVVHNDSRRQSVSVENTFDKDLPDLAACLEQLPALLEQLATRM
                     ARLDASYRPDKPFVKIKFHDFSQTTLEQGGARRDLESYTRLMSAAFARGNKAVRLLGV
                     GVRLHDLRGKHEQLELFAP"
     gene            complement(18427..19869)
                     /gene="rhlB"
                     /locus_tag="VFF22_04955"
     CDS             complement(18427..19869)
                     /gene="rhlB"
                     /locus_tag="VFF22_04955"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HZX16201.1"
                     /translation="MLKALKKIFGKAEGEQPSSATPSPVTPSAPRVQPDANADKSSRS
                     ASPRPTEAAEKPLQNKEKPAKPRRERPAKPVDTWKLEDFAVAPAEGKTRFHDFKLSPE
                     LMHAIHDLGFPYCTPIQAQVLGYTLSGRDAIGRAQTGTGKTAAFLISIITQLLQTPPP
                     KERYMGEPRALIIAPTRELVVQIAKDAADLTKYTGLNVMSFVGGMDFDKQLKLLESRF
                     CDILVATPGRLLDFNQRGEVHLDMVEVMVLDEADRMLDMGFIPQVRQIIRQTPMKGER
                     QTLLFSATFTEDVMNLAKQWTTEPAIVEIEPENVASDTVEQHVYAVAGSDKYKLLYNL
                     ITQNDWIRVMVFANRKDEVRRIEERLTRDGISAVQMSGDVPQHKRIRALEGFREGKIR
                     VMVATDVAGRGIHVDAISHVINFTLPETPDDYVHRIGRTGRAGSSGTSISFAGEDDAY
                     ALPAIEALLGRKIACEMPSDELLKPVPRKH"
     gene            complement(19974..21137)
                     /locus_tag="VFF22_04960"
     CDS             complement(19974..21137)
                     /locus_tag="VFF22_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HZX16202.1"
                     /translation="MRKILIALLIVLLISAGSVFALPSLFDRKMNSVESPAPYPASES
                     ANQLHQQLFIADLHDDVLLWERDLLKRYDFGHSDLPRMLEGRVGLQVFSTVTKTPRGI
                     NYERNSGETDNITLLAMAQRWPKETWNSLLQRALYQAHKLNEASANSDGRLVMIKSRG
                     DLASFVSAWQKDPQRVAAILATEGLQPIEGKLENVDALYDAGFRITGLTHFFDNEIGG
                     SAHGLEKGGLTPLGRQVIKRLEEKAMLIDLAHASRPLIDDVLAIATRPVLVSHTGVEG
                     TCKGTRNLSDQHLKAIAATGGVIGIGYWDAAVCATSVEAIVKAIRYTAGLIGVQHVAL
                     GSDFNGTIHAPFDVTGLPQLTEGLIKAGFSHDEIAAIMGGNVQRLLLASFPEN"
     gene            complement(21368..22306)
                     /locus_tag="VFF22_04965"
     CDS             complement(21368..22306)
                     /locus_tag="VFF22_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZX16203.1"
                     /translation="MKNAKWQADLQQLRNLRLFNNVAASSISQLLKEFRACDLEAGEV
                     LLSPFNRNQYLYLLLKGQLKVYLGSLESQAVSTLKVGDCAGEISFIDNEHPSAYVVAT
                     EPCCVLRLHRESLFNLFQQSPELMQNMLELLCDRVRKGNRIILDSEQTANIDALTGAY
                     NRRWLEHIFERESTRSAFNGKPLCMLMLDVDHFKAYNDQHGHLAGDYALCLVAHTLRS
                     QLRPKDSLTRYGGEEFVILLPEISLDEARNIGERLRLALRNISSFHSPIGVLPGVTVS
                     IGLAQMQPKDSLQGVIARADSALYQAKQQGRDCLCG"
     gene            complement(22504..24405)
                     /locus_tag="VFF22_04970"
     CDS             complement(22504..24405)
                     /locus_tag="VFF22_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009044206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HZX16204.1"
                     /translation="MPAIPAIKTLRDIQLIEQTPLAERDLPASTYELIRRSAARQADA
                     PALSFILQGTADEAAYRLSYGQLLGKITQTANAFHRLGLRPGKAVSFLLPNLPHTHFA
                     IWGGEAAGIVNAINPLLDPEHIAELIQASDSELLVTLAPFPGTDLWAKVEGLREQLPT
                     LKAIICVDMANLLPEPQSTAIKAQRGPLPDGVLDFDELIASCPDDHLESGRVIAPDDI
                     ASYFHTGGTTGTPKLAPHSHGNEVAMAFSMNLVTRFEPGDVTLCGLPLFHVNGVIVTG
                     LTAFIGGAEVLLATPQGYRNANLISNFWTIIERHKVSFFSGVPTIYAGLLQVPSEGHD
                     LSSLKYALCGAAPMPVELIRQFESKIGLTLIEGYGLTEGTCGSCANPPAGERRPGSIG
                     LPMPYCEVAIKILDEQGNYLREANRNEIGNLCIRGATVFKGYLQSSKNAGIWVDGDWF
                     NTGDLGRVDADGYIWLTGRSKDLIIRGGHNIDPQMIEEALHKHPAVAMAAAVGKPDEK
                     AGELPVVYVQLKPGAKVSEAELLAHAAEHIPERAAIPKDAWIIDAIPLTAVGKTFKPA
                     LRFDAITRVYQAALAELDSRLRVEVLSDDKRGQVAHIYLPAAQAALADAVSKRLAGFA
                     VAIELHPSE"
     gene            complement(24428..25087)
                     /locus_tag="VFF22_04975"
     CDS             complement(24428..25087)
                     /locus_tag="VFF22_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX16205.1"
                     /translation="MSEPLILQPTLAADSCVIWLHGLGADRYDFMPVAEMLQKRLHNT
                     RFVLPQAPTQAVTINGGYAMPSWYDILAMSPARAINREQLEASAQQVIALIEGQRDSG
                     IDPARIVLAGFSQGGAVVLHTAFLRWQFALGGVIALSTYAPTFSDDIQLADTKRQLPV
                     LCLHGTFDDIVLPAIGRAAHDYLSASGVSVQWRDYPMGHEVVNEEIRDIADWLEQRFT
                     S"
BASE COUNT         5218 a         6479 c         7393 g         6037 t
ORIGIN      
        1 gcccggcgcg ccggtgatcg aggcgtccag cggttccacg gctatctccg aggcctattt
       61 cgcccgtctg ctcggcctgc catttatcgc ggtgatgccg gccagcacct cgaaggagaa
      121 gatcgcgcag attgccttct acggcggaca gagccatctg gtgacggacc cgacgcagat
      181 atacgccgag tccgagcgcc tggcgcgtga gaccggcggg cattttatgg atcagttcac
      241 ctacgccgag cgcgccaccg actggcgcgc caacaacaat atcgccgagt cgattttcca
      301 gcagctgcgc agcgaacgct tccccgagcc gagctggctg gtttccagtc ctggcaccgg
      361 cggcactctg gccacattag ggcgctatgt acgctatcgc cgccacagca cccaggtgct
      421 ttgcgccgat gccgagcgtt cggtgttctt cgattactac cgcactggcg atgtcagcct
      481 gtgcttgaac cacggctcgc gtatcgaagg tattggccgg ccacgggttg aggcgtcctt
      541 tctgccgtca gtgatcgatg ccatgtgcaa ggtgccggac gccttgtcgt tggccgccat
      601 gcattacctg gcgcagcgcc tgggccggaa agtgggcggt tccagcggca ccaatctgat
      661 tggcgcattg ttggtggcgc aacggatggt cgcggcgggc gaatccggtt cggtggtggc
      721 gattctctgc gatggcggcg agcgctatgc cgacacttac tatgatcagg cctggctcag
      781 cgctcagggc tttgacctgg caccgctgct cgaggcagtc gccgcctgcg ccgaacgcgg
      841 cgtctccttg cctggtgact tgcctacggc cagcttgtag cccggatgca atccgggaag
      901 gggtttgcgc cggacatcgc ccccggactt catccgggct accagagctg cgggcttttt
      961 gacaaaaggg agggtaaatg agcgatttcg aaatacgcca agcacaggag ctggacatcg
     1021 ataacctctg cgcgctgatc ctggagcatg ggcccaatcc ttggaattac ctacctccag
     1081 acgaagtagc tgcccatctg cagggcatcg ccgacggagc ggtgcaggcc gtgttggccg
     1141 agcgcgcggg agaattgcag ggctttgtca gctaccagct atccaggcat ttcgagcgct
     1201 atcaacccga agcgcgacgc ggccaacctc acggctatat ctgcgaagcc gtcacccatc
     1261 gtgcggtggc gggccagggg ctgggctcgc ggttgttgca acacgcggtg atgcagctgc
     1321 gtgagcagga cgtgagcgat atctatatcg accgccacga agaaaatgcg gcttcggcag
     1381 gcatgatgcg taaagccggc ttcgtcgaga tcgaaaccta tgctgacccg cagcgcaggc
     1441 ccaatggttc cggccgtacg accctgtgtt gtttgcgttc agcttgatgc ccaacagcgc
     1501 cgccggcgcg gcgccaggag aggaggcgga tgattcagtg caagcgggta tacgaagcca
     1561 gctcggcagc ggatggtcgg cgagtgctgg tcgaccgcct atggccgcgt gggctgagta
     1621 aggcgaagtt gcagctggat gcctggttgc cggaggtggc gccatccacg cagctgcgcc
     1681 gccagttcgc ccatgagccg gaagcgtttg aggccttccg tgcccagtac cgtgtcgagt
     1741 tggcggccca tcctgaatat tggcaagggc tgctggaacc tgctgagcag ggcgtattga
     1801 ccctgctgtt cagtgcccgt gatacgcagt tgaacaacgc ccaggtcttg gccgagtttc
     1861 tcgaggacga gctggagcgt cgtggcccgt ccagctcgcc ggtttgctat accgacagga
     1921 tgtaaggcgc ggcttagctt tcagcaatcc ccagcatgtc gcgaatcagc gcttcggcca
     1981 cgcgaatacc atcgaccccc gcggagagaa tgccgccggc gtaacccgcg ccttcccctg
     2041 cggggtacag gccgcgaacg ttcaggctct gcatatccgc gccacgggtg atgcgcagcg
     2101 gcgaggaggt gcgggtctcg atgccggtca gtaccgcgtc atgcaggtcg aagcctttga
     2161 tctgcttgcc gaaggccggc agcgcttcgc ggatggcctc gatggcaaag tccggcagcg
     2221 ccagggccag gtcacccagc ttgatccccg gtttgtacga gggctcgacg ctgcccagtt
     2281 ccgtggatgg cttgccggca ataaagtcgc cgaccagttg ccccggcgct tcgtaggtgc
     2341 tcccgccgag cagataggcc tgggactcca ggcgctcctg taactccaca ccggccagcg
     2401 ggctacccgg gtaatcctgc tccggggtga tgccgacgac gatgccggcg ttggcgttgc
     2461 gctcattacg cgagtactgg ctcatgccgt tggtcaccac gcggttcggc tccgaggtgg
     2521 ccgcgaccac ggtgccgcct gggcacatgc agaagctgta gaccgagcga ccattcttgg
     2581 cgtggtgcac cagtttgtaa tccgcagcac cgagcttggg atggccggcg tatttaccca
     2641 agcgcgcgcg gtcgatcagg ccttgtgggt gctcgatgcg aaagcccacc gagaacggct
     2701 tggcttccat aaatacctcg cgcttgtgca gcatgcggaa ggtgtcacgc gagctgtggc
     2761 ccagggccag gatcacctgg cggctgagaa tctgctcacc gctgtcgagc accacgccct
     2821 tgagttgacc atcctcgatc agcacgtcac tgacgcgttg ctgaaaacgc acttcaccgc
     2881 ccagggcctt gatttcctcg cgcatggtgg cgaccacgcc ggtgaggcgg aaggtgccga
     2941 tatgcggctt gctgacgtag agaatctcgt ccggcgcgcc ggctttgacg aactcatgca
     3001 gcaccttgcg cccgtaatgc ttgggatcct tgatctggct gtacagcttg ccgtcggaga
     3061 aggtgccggc accgccttcg ccgaactgca cgttggactc agggttgagc acgttcttgc
     3121 gccacaggcc ccaggtgtcc ttggtgcgct gacgcacttc ggtgccgcgc tccaacacga
     3181 tgggcttgag cccggcttgg gcaagaatca gcgcggcgaa aatcccgcag gggccaaagc
     3241 cgacgaccag cggacgctcg gcgaggttct cgggcgccat gccgacgggt ttgtaggcgg
     3301 tatccggggt gacgctgata tgccggtcat cgctgaacgt ggccagcagc gccgcttcat
     3361 cgcgcagctc gcagtcgatg gtgtagataa agtgcatttc agtggatttc ttgcgcgcat
     3421 cgtagctgcg cttgaacagg ctgaagtcga gcagctcgct gtcggcaatc cccagacgct
     3481 ggatgatggc cgggcgcagg gcctcgtcgg gatgatcgag gggcagcttg agttcggtga
     3541 tacgcagcat gagggacgat ccactttcag ggccggaggc aacccggcag cttttaaaag
     3601 gcggcgatta tacccgtgaa tatgcaccgc tcgcttgtgt ggagcgactg taggagccag
     3661 cttgctggcg atgccgttga tctacaagga tcgcctgcaa gctggctcct acatgttgtt
     3721 ttgccgctac aacttggcgc gtctatgtgc ttaatgcttt acccaggcca gcatcacatc
     3781 gagcatacgg ttggcgaagg cccactcgtt gtcataccag gccaagacct tgaccagatc
     3841 gccctgcaca cgggtgtggc tgaggtcgac cacgcaggac accgggtagc cattgaagtc
     3901 gctggaaacc agcgccagct cgttgcattc catcactcca ggcggcaact gcaaggcgcc
     3961 ggcctttagc gcggcattga tcgcttcgtg gctggtcgcg cgttcgctgg taaaggtcag
     4021 gtcgaccagt gacacgttgg gcgttggtac gcgtatcgac aacccatcca ggcggccgat
     4081 cagctccggc atcaccaggc cgatggcctt ggccgcaccg gtgctggtgg ggatcatcga
     4141 cagcgcggcg gcgcgggcgc ggtagaggtc gctgtgggtc ttgtccagca ggttctggtc
     4201 attggtgtag gagtggatgg tgttgaccag gccctgacga atgcccacgg cctcatgcag
     4261 taccttggcc attggcgcca ggcagttggt ggtgcaggag gcgttggaaa cgatctgttg
     4321 atcgcccagc agttggtgat tcacgccgta gaccacggtc aggtctgcgc tgtccagtgg
     4381 atgcgagagc agcacgcgcg ggctgccggc gctgatgtgc tgttcgacct gggcgcgctt
     4441 cttcagcttg ccggtgcatt cgagcaccaa atccaccgca tgctcacgcc agggcagatt
     4501 ggctacttcg cgttcacgca gcagctggat cgggtggccg tttaccaaca aggtatcgcc
     4561 gtccaggctg atatccccag gaaagcggcc gaaggtggag tcgaagcggg tgaggtgggc
     4621 gagggtgctt tgctcaccca ggtcattgat cgcggtgagg tgaatctgct gctccagttt
     4681 gcgctcgaac aacgcgcgca ccaggcagcg accgatacga ccatatccat tgatagcaat
     4741 gcgcagcata acgatatccc tttggctgtc ttcccttgca gcttaggcca atcgtctgcc
     4801 gctcggctga tctatctcag ctatctgatt gctaatcgtt gcgcgcgccg ccgaggtagg
     4861 cacagccccg cagcacttca ctattaagac gcaactcggc gctcaggtgc tgcaccgcgc
     4921 cactcatgcc gtcgacgcag cgctggcggg cgacccacag atcaaggcgt tggccgttgg
     4981 cttcgctggt gaggttgagg cggccttcgg gcaattgctc ttccaggtaa ggcagtgctt
     5041 gaggctcttg gcctgggcgg ttgaggatca ggccctgatt gcttaccgcg atattccaat
     5101 ccggctcgtg gccgctggca cgcaggatgg tacgggcgaa attgatatct gtgcagccat
     5161 ggccttcagc ctgtacgcgg tacaccaggc tcggcacgaa ctggccatcg acacctttct
     5221 gcgcattggc ctgcagctgg ccgcgcatat cgacatacag cgttgtgtgg ccgtcagcga
     5281 acagcgtgct ggcatcgctg cttagctcgg ctgcgctgtc ttccttgagg ataaattgac
     5341 gttgttcctg gcaggggcga aacagcaact ggcccgcatt caggctcagc tcgccctgca
     5401 tgcgcgtctg ctcgatcagc ggctcggcgg gtttgtcggt aaacacctgg caactggcga
     5461 gtagtggcaa caaggcaaag caaaaaggac ggctgaaacg catcgtggca ctcctgaaaa
     5521 gtgcgctcac gttacctagg ccccaggcgc atcacaaggg catgccgccg ctgggatagc
     5581 gggttgtttc tgctacggaa cagggcagat cacaatgttg ctcctcggga tcgattgaac
     5641 gcttggttcc cacagcacga actacagaat ggccagggca gtaaagcctg aaataggctg
     5701 aatatgagaa tgcaaccgtg ctgacgacag atgcaaaagt aaggctttgc ttactgcttg
     5761 tggggagagt ccatatacac ttgttaggca tgtgcatctt caacaaggaa tactttgctg
     5821 ccaacaggaa gttgattttt cgcaatgtta cttggcacag agaaaggggc atgattcatt
     5881 ggcttgccac gactaatcat ttccatgcta ataagtgtgg ttctcacatg ctcaccggaa
     5941 ggagtttcga ttagcaattg agcaccaatg ccaacaggcg tcgggaattc ataggggatt
     6001 ccgggcacta ccacgcagcc acgaccggtg atgtaaaaca cgtcttctat tgtgcagatt
     6061 agctgcatag caatgtctaa cgtttggtta aggggcgggc tttagcccgt cccagtgagc
     6121 gaagcgagcg attttaaccg ctggttaggc gtgtgcgctg atttcaggag atttaataag
     6181 gcgcttgctt tttagccagc cctctacggc gattgctaaa gaactctcaa tgttaaacat
     6241 gctgaagctg acgtatagtc tgccttgtaa gtctgacggt agtgaattaa cactctgctc
     6301 cttcagcaag catacatgtt tgccttgtcc cataagatag ccaatttcta aagatacatt
     6361 cgcgttgtga ttttcgtttt ctattctgtc atgtacagaa aatgcgaatt tcgagccgaa
     6421 aatgtatgtc tcaatgtttg agagcacgtc atcggaatac gctctatcat ctgcccttaa
     6481 taagttgaag ccatgttgtg atagtatttt ccttaaagct gcgtggactt cgcggtgaaa
     6541 tggggtttct cgaaatgaca ttattaagaa gcaatttttc ctataatctg gccagtctga
     6601 aaataggtcg aagagtgccg acttaagtcg ttgtggggca gagtcaactg agttttttct
     6661 gaattcattt tctctataga gatttagttc ttcacgaata tcatcgaaag ttatccagag
     6721 ggatttgttg tagcttccaa gtatgtgaga aagcgtgttg tatgcaacgt gtaatgcaga
     6781 gtcagcccag tccaaatttt cgaggacggg gttgatttga tcgtggtttg caaggaatcc
     6841 ttctatcaca tctgatttta aaatttcctt tcttccatgc cttatggcgg tggctatagc
     6901 gctgtcaata aatccctctc ggcacccaag ccttttccct tcagaggatg agtaaggagg
     6961 gagattgtca tcatatttat catcctccct tgtaagggct tctaatgtgc atatgtgctt
     7021 tgcctcttca gggtcgcaac cctttcttag catgaggcct cttagatctg cgaatgtatt
     7081 aattattgcc agaaggaata catagggata aatgtctctg ttgtatttgc agcctatgga
     7141 ttcagcttct ctaaaaacgc gttcagcttc attcgacaat ataaataatt tcttttcgac
     7201 atagcttttc aggtcgctta ttgcgtcatc tactgtcatg gtgtgcttca tgaatgatat
     7261 atgcctaacg tttggttaag gggccggctt tagccggtcc cgagtgagcg aagcgagcgg
     7321 cttgaaccag ttgttaggct ccaactgcat tgggctgtat ctgaggttta ggcggaataa
     7381 ttgaacgtag ttctgagaag tcattttctg agcgagccgc aatttcagac caggcgcgtt
     7441 cgaatgcagg gcggcttata ttggatttca ttccgtcctc ccagaacttc caggttttat
     7501 ttgaaatgcg ttttgtttgc tttagaaata cttgctcatt gcaaaggtca aagtaatgat
     7561 acatttcgtc aaagctagca gcatactcct cgtccgttaa tggctctcca agtaatgctt
     7621 tagtcggtag ccttgccgct agttctctat attcctttgc tagggaatct tcgaacgtag
     7681 tttctgcttg tttttttgat agccatatct gccatgcggc gaatatgact gcggccgccg
     7741 ttgctagatt cgctgcatct gataagtatg aggccacagt atttctgccc ttggtgccta
     7801 acgattaagc taaggggccg gcttcgccgg tcccagcgaa cgaagtgagc gctttgagcg
     7861 cattgttaga tgcactcact tacttagcca gcccagcaag gcacctaaag ccacgcccag
     7921 tagactgaat aaaggtgatg atagccagcg atactctcta ttttgctcta tggctcgcgt
     7981 atgtaggttt atggctgtgt tgattaaaac tgatatgtcg gagctattta gcggtgctcg
     8041 ggaaatacga gcactatctt cattgcttag agcttgatac tcagcaagtg atagctcccg
     8101 ctgctgatcc actgagtacc gcctacggta gcttctacgc caagctaagg atgctttcac
     8161 tccggcggtg tctagcctga agaattctgg gtgttctaga aatactttct tccagtgctc
     8221 tgctttgctt ttgtctccgc tgattcgcgt ggcccatcct tcaaaatcca atttgtaata
     8281 gccataacta cccatgactt ggatagctgc aattacatct cctaggcgat tttcgccata
     8341 aaggtaaggt gattttgttt tggcagccat gcggtgctcc ttgcagtgca tctaacgttt
     8401 ggttaagggg cgggctttag cccgtcccag tgagcacagc gaacgatttg aaccatttgt
     8461 tatgcaatta caactgtgcc tttcctgacg actgctgctc ttggattggt tcggctcgac
     8521 gatatagtgc caaaagaatt ttcaactgta caagctcatt tccgttcgtg attttttgta
     8581 cggcacttac ttgttctgga cgcaagttga attcggaaga taggcgccta attgattgct
     8641 gtataaatac gatgtcgtct tgcattaatt ttgactcggc ctcttgaacc ttgatatggc
     8701 ctaaccttct tctgtgataa ctttttacta ttgtgtcgta tgttttgttg atggccaatg
     8761 ctgaggcgca tagcgcacta actaaaatac taacatctga agcgatagct ataacgccac
     8821 tgagctcaat aaaacccgga gaggcatatt gaattctatt tatttggagg cggtattcat
     8881 ctggagttaa gccgtaggaa cctttgaaaa aattaacaac actgtaaccg ccaccccatg
     8941 ggtaagtgga aaaatctagc ccgacgtata tcccttcatc tacgcacttg agaacataga
     9001 aaaagctata tagctgaacg tattctttgg tgactacgga aagatcttgt agctcccagc
     9061 gtttatccag ctttatttca atttttccaa tgtccatacg gtttctcaat aagtgcataa
     9121 cgataaagct aaggggcggg ctttagcccg tcccagtgaa cgaagtgagc gatttgagcg
     9181 ccttgttagg cattccgctc gccattagtt gaaagtctat gcttttgcat aagtccaagc
     9241 cccctgtcct gagttgcaaa tagcagcgct tgaaaacgct ctatatcatc aatcaatgaa
     9301 tctagaactg ggagcttgaa gtgtgtgcga atgaacttga gcacatgatt gtaccgggta
     9361 gcggcagacg gatgtgattc tgactccaaa aacatgctgc actttaaatg tgaaataacg
     9421 gtgtagaaga gcaatgcccc tagcgctacc tcaaactcag cctgatccga gctatcgcca
     9481 aacgtggacc agagagggag cgctagaccc aataaatcag cttcaatctc tttacgatgt
     9541 tttgcggaca atccctgatt tagaaaagga gcaatacgga tttccagcag ttcagaggtt
     9601 gttgcgtctc gtccagtatg gcctagtagg tgatgagcta tttcgtggcc aagcactacg
     9661 cgatccatga gagttgcaat tctggcagcg ttgtccgcga gatgatctga tggatatatc
     9721 aatgtttcat ctttaagtcg tatctcgttg ccgtttattc cccactccaa ctgcgcgttt
     9781 gcaagcttat ttaggccttt tacgactcca gccattgaca gcaggacatc ctcagaataa
     9841 tccgggagct cctgacgtaa cgcacaaaga tttatgaatt ccccgaagag aattgcatac
     9901 tgaaaacaaa gatcggatag ccctacgtag aagaaaccca aatccccttc gaatcgtccc
     9961 ccagcatgaa ttgctccagc gttagctaag aaatcttttc gtttagccac gaacatacga
    10021 gaaatactgt ctgaaaacgt tcccgatgtg ctcgcgataa aattctcaat atagctttca
    10081 atcaggatgt cattgctcag atttggtacg tgcccaacaa tattgcttag gtcttccttg
    10141 gagcttttct catcgtaaat tgcgctctga tgggcctgtt gatagttctt tccggcctca
    10201 tctggcgcga tttctgaata gtaaaattcg cgtgctgaaa gcatttctag tccgttctcg
    10261 gatgcctaac gattaagcta aggggcaggc tttagcctgt cccagtgagt gtaacgaacg
    10321 acttgagcgc cttgttagcc aacctgccgt tcgcggtttt aaattaccga aattctgctt
    10381 tttgctgctg cgcggtgaac cagaaaaacc gcgcaaaatt ccttgtgacg atgttgctga
    10441 ctgaccgcgt ttttgaatgt gcccggatgg tgaaggcggc cgttcagtga acgcattttt
    10501 accgacttgt gaaacataaa actgcggagc gatgcagcgg attaacacgg tgcaaaaccc
    10561 aataagccaa ccacgattag gctaacgttt aagctaaggg gcaggcttta gcctgtccca
    10621 gtgagcgaag cgaacgattt gagcgcatag ttagccgctt aaatacgtta gataaacgtt
    10681 tgctaatggt agcgctattg ataaggccgc ccagagaagt ggaagatcat aatcgataaa
    10741 acttgattcg ttggaagcgt acttgatgta cgccaagacc tcaggaatga aaacagtacg
    10801 gtatgggatt tgtatcactc gttcttctag gtcgccttca ttgtttattt ggtatgcaat
    10861 atttcgtttg aagaaatggt ggcgaacctc tggatagcga cttcgtgttt ccatgaattg
    10921 ggggtctttc tttatggctt caagtatttt ttcgttatct ttaatgtaca aatttgctct
    10981 ttttgcgatg gatggattag attttttgtg tgcatttata aagcacgccg ctatgtatat
    11041 aacaaaaata aagatgaaaa attgtattgc ataggtgttg gtaatttttg ttacaacgaa
    11101 aggtatttcg atttcggata tttcaatgct tgcaatcgaa ataagcagga gaagactgct
    11161 tgaaattagt agggttcgct tgatttttga gatttgttcg tccatgcgat aaaggacttc
    11221 cttaaggggc taacgtttgg ctaaggggcc ggctttagcc ggtcccagtg agcgcagcga
    11281 acgatttgag ccacttgtta ggcctgactg acccaacacg ctagccgcgg caacgcagaa
    11341 agaaaaggca gacgctgccg atgatgtgag tgtaccgagt aagttatttg cagtggtaca
    11401 aggcataaat gcaaagccgc ttactgatgc aaatgacggc actttcatga tgacgactcg
    11461 gtgtcgcgga tgctcagcga tattggggaa atcttgacga ctcagcagta cttccatgtg
    11521 ttcacccagt gagtgcttcc acgtgcctaa cgctggaggt aagcggccgc gtcccgcggt
    11581 ccgcttgacc gacttgttat gctgccacaa tttagtatgg cctttctaag ccgctcggtg
    11641 ctcaaagaaa aagtgctaat agaaagcaca aaatttaacg gcggaaacgg cagcaccacg
    11701 aaaccatggg tggcacgaac aaactagatc atgccacgta aagcgcaaaa gcggggtatt
    11761 taaaggccgc taaaaattac cacgttgcga acgaaactat cgaacctgga gcggcacgat
    11821 ggaaatctcg ccattagaat ggcaaacaac ggtgcccata gaacccacta gaccaatcga
    11881 atcaaacaag attcgccgac aaccactacc ctgctttgaa atccaccggc tattttttgc
    11941 cacgcataac gtttggttaa ggggcgggct ttagcccgtc ccagtgagcg cagcgagcga
    12001 tttgaaccat ttgttagccg gcagcacgca gcatttgcgc gaacctctcg gcctcccatt
    12061 cctttctgtc tgaaacttga ccatcaccga gttcaactac tcggattaca ttgtctgacc
    12121 tcaacacaac gtcaaccgag aaaaaagggc ttcggactgt tctagcacaa atattaacaa
    12181 tgtccggaac attaccagct gccgcgtagg gtttgccgtt aagaacaaag taccggcgct
    12241 ctgtgccttc tatgtagttt tcacgcattc tgacgcaaat accgccttcg atttggccac
    12301 gatatttctg cattagctga acaactgccc cgattttgtc aggtgtgtca accaaagatc
    12361 cgccagcagt gttcagcgac ttgacatagt cttttataaa gaaccctggc cagtttaagt
    12421 gagctagctc tttttcaaag ttcgcgtcac tttttagaac tatggtttcg ctagttaggc
    12481 cagttaggtg tgggtaccat tcaggtaggt gatggcaaag cttgtattgc tcagggcttg
    12541 tgaatgttat tacccctttg ctgtgcagcg cagatgacaa gcgttcgtat agttctggtg
    12601 tgagcatcca gccacgatat aagactatct cgccatgctg gacttctgga cggattttta
    12661 aaatgccaga ttcaaaatct tcaaaggaaa ataatgatgc actgatgcca gcagataacg
    12721 cagcaccgaa ttcttccttg taactctcat ccgggagctt ggtgtcaagc gggtcgcttg
    12781 gatagagtaa acgcatatat tcctctgctg gctaacgttt ggttaagggg cgggctttag
    12841 cccgtcccag tgagcggagc gaacgatttg aaccagttgt taggcttcac tttttacgac
    12901 agctatttct ctgattgtaa gatccgattt atttgtccag agatcttttc tcatggctag
    12961 cactgcgctc aaaaaatttt ccctactagg ctgctctatg aaagctttga cactgagtac
    13021 aacaggtttc gctgcgtaga gaatcatttc gtggtactcc gctttaagat actccatgac
    13081 atctgacgct gaatcaatgt ctgcctgccg gctagatagg tatttaatat ttttggggtt
    13141 aaagtaaaca tccatatata acaggcatga tttgtagcgg cgctctttgc tttcgagtgc
    13201 tttgaatgca agttctttat ttctggtctg aacgttggta atgattccgc cgattccgac
    13261 aaggccgagt agagcaagaa taatctcaat gggactcata tgatgcctgc tgtgatgcct
    13321 aacgtttggt taaggggcgg gctttagccc gtcccagtga gcgaagcgaa cgatttgaac
    13381 caattgttat aggaactcac cgcgccctct attttgtacg gtaataattg tagatgtctt
    13441 tagcctttga gcaaccataa aaagccaagt agcagaaaac tacaatggct attgtaggtc
    13501 ccgccgcatt tattccagtc atataagtta tataaattgt gctaaaggtg atcagagcca
    13561 cgtaacttgt gagcgttctg ttttttgcag acagaattaa tggcgctgct gagagagcaa
    13621 ttatgtggat tgaaattgtg gtgatgattt ctaaaattag ttccgttggg attgtgtcgg
    13681 ttcgtgcctg ggtgattaga ataatagctt ggtacgagta ataatttaaa cccaacacga
    13741 tagctgtgct gataaaaagg attggtgcgt attttttaat tgcgttacac atggaggtag
    13801 aatgattcct ataactataa atagacacca catggtgccg taacgctttg cgggcatggt
    13861 ggataatatt cttgtccagt tgcggggcgc cgtctaggct gaggtttgcc gctatgattt
    13921 ggcaggatag ggagttatac accatggatg gaaagcctcg gttactcgac caggttcgcg
    13981 agcaagtttg cctgaaacac tactcgattc gcactgaaca cgtctattgc gaatgggtga
    14041 agcgctttat tcgttttcat cgttaccggc atccagaggg aatgggggcg gctgaggttg
    14101 aagcgtttct ttcagatttg gcggtgcgca agaatgtttc ggcttcaacg cagaatcagg
    14161 cactcgcagc gttacttttt ctgtacaagc aggtcttgaa gttggatttg ccttggttgg
    14221 gcgaagttgt gcgtgccaaa aaaccaagtc gtttgcccgt ggtgttgagt ttggcagagg
    14281 ttcaacagat tttggcgcaa ctggaggggg aggttggttt ggtggccaat ctgctttatg
    14341 gctcgggtat gcgcttaatg gaagtgatga ggttgcgggt caaggatatc gattttacgc
    14401 ggggggaaat tctggttcgt gatggcaaag gtatgaagga tcgcgtaacc gttctgcctc
    14461 gctgtttgat cgagcgcctc aagcagcact tggtgttggt cagggctcgc cataatgctg
    14521 aattgaagga gggcaggggt gatgtctact tgccattcgc gcttgagcgc aagtatccaa
    14581 atggtccctg ggattggcgt tggcagtatg tgtttcctgc tgctggcttg tcggtcgatc
    14641 cgcgtagcgg caaggtacgt cgccatcatc tggacgagaa gcgcgtgcag cgtgcgttca
    14701 aggcagcgat taagcgtacg ggcattgtga agctggcgac accgcatacg ttaaggcatt
    14761 cctttgccac tcatcttctg gaaagtggcc aggacatccg cactgtgcag gagttactcg
    14821 gtcatgcaga tgtgaaaacg accatgattt acacccatgt actgaaccgt ggtgggcttg
    14881 cagtgttgag ccccttggac cgcgcgtaaa aagccctgca tcggcagggc tgtttagtga
    14941 acgatagacc gcttggcggt tagatcagct ctgcccacat gtcgtattca tcggcatcga
    15001 cgatgcgtac gcggaccctg tcgcctgggt tgacgttggt gctggcgata aacacgctgc
    15061 cgtcgatttc cggggcgtcg gcgtagcagc gggcaactgc gccctggtcg tcgacttcat
    15121 cgatcagcag gtccatttcc ttgccgactt tcagctgcag gcgagcggta ctgatggcct
    15181 gctggtgagc cataaagcgg tcccagcgat cctgcttgat gtcatccggt actgggtttt
    15241 caagcaggtt ggccggtgcg ccttcaactg gtgagtattg gaagcagcca acgcggtcga
    15301 gctgggcttc ggtcagccag ttgagcaggt actggaagtc ttcttcggtt tcgccgggga
    15361 agccgacaat aaaggtcgaa cggatggtca gttccgggca gatctcgcgc cagttcttga
    15421 ttcgcgccag tgttttgtct tcgaaggccg ggcgtttcat ggctttcagc actttcgggc
    15481 tggcgtgctg gaaggggatg tccaggtacg gcaggatctt gccggcggcc atcagtggga
    15541 tcagctcgtc gacgtgcggg taagggtaga cgtagtgcag gcgtacccat acgcccatgc
    15601 tcgatagtgc ttcgcacatt tcggtcatgc gggttttcac cggctggccg ttccagaaat
    15661 cggttttgta tttaacgtcc acgccatagg cgctggtgtc ctgggaaatc accagtagct
    15721 ctttgacgcc ggctttaacc aggcgctcgg cttcgctgag cacttcaccg accgggcggc
    15781 tgaccagctt gccgcgcatc gaggggatga tgcagaagct gcagctgtgg ttgcagcctt
    15841 cggaaatctt caaataggca tagtggcgtg gggtcagttt gatgccctgt ggcggcacca
    15901 ggtcgatcag cgggttgtgg tcctgacgcg gtggcacgac atcgtgaacg gcgttgacca
    15961 cctgctcgta ctgctgcggg ccggtaaccg atagcacgct ggggtgtacg tcacggatgc
    16021 tgccttcttc cacgcccatg cagccggtga cgatcacctt gccgttttcc ttgatcgctt
    16081 cgccgatcac ttccagggat tcggctttag cactgtcgat aaagccgcag gtgttgacca
    16141 ccaccacgtc ggcgtcctga taggtcgaca ccacttcata accttccatg cgcagctgag
    16201 tcaggatgcg ctcggagtcc accagcgcct tggggcaacc gagtgataca aagccgactt
    16261 tcggcgtggc gggggtggac atggcgttaa cctcaggcgt gtacggatgg atgctgggcg
    16321 ctttggcgcg cctctgatca aaaagtgcgc aattctagcg gcgacgctgg caattgacca
    16381 gcgctgagcg tcgctttatg cagtgggggt gtttatctgc gaagccagga tacgccccag
    16441 gttgagcttc gagcggataa gcgatgagct gctccgatag aggcaggcag cgcctaggtt
    16501 ctattgagtt ttatcgctta aatcgcgcac aaacaaaaag gccactcgat tgagtggcct
    16561 ttttgagtat ctggttgcgg gagcaggatt tgaacctacg accttcgggt tatgagcccg
    16621 acgagctacc agactgctcc atcccgcgcc ggccattcta tagagaaatc gcattctgtc
    16681 aacaaaatag tgtttttaat caattgctta ggttgtgcgg ggcggcagaa acaaaaaagg
    16741 ccactcgatt gagtggcctt ttttgagtat ctggttgcgg gagccggatt tgaaccgacg
    16801 accttcgggt tatgagcccg acgagctacc agactgctcc atcccgcggc gccaattcta
    16861 gcgtctaatt ccgatgtgtc aacctaaagg tggatataag ttgtttttat tcaggtgctt
    16921 agccgtgcgc tgtgttggtt ttaccctaca gaaccctatg ctagagcctg attgggcggt
    16981 caggttcgct ttcggcatag cccgcaaccg ggctgttggg ctagagtccg tgcccattaa
    17041 atgggaggaa agttatgagt gttgaatgca aagcctgtgg tgcccaggtg ccagcagatg
    17101 ccaagacctg cccgggttgc ggcgttgccg tggcagcgaa gaccaagttg ctgccgttgg
    17161 cggcttttat gctgatcgtc atcctgatta tccagtctta tatgagtaag tctgaggatg
    17221 cggcgagtga ggccaagcct gcggcgcctg taagctcagt cgagcagtag cagcgggatc
    17281 ggtgggctcg gcgatgaaca gccgcaagat catccatgtg gactgtgatt gcttctatgc
    17341 ggcaatcgag atgcgggatg acccgagcct ggccagtaaa cccttggcgg tcggtggctc
    17401 ggcagatcgg cgtggggtga ttgccacctg caactacgag gcgcgcagtt atggtgtgcg
    17461 ctcggcaatg gcttctgggc aggcgctgaa gttgtgtccg gacctggtga tcgtcaagcc
    17521 aaggattgat gcctacaagg cggtatcgcg ggagatccag gcgatctttc gcgattacac
    17581 cgagctgatc gagcctttgt cgctggatga agcctacctg gatgtaaccg acagcccgca
    17641 ctttgccggc agcgccacgc gcattgccca ggatattcgt cgacgggtct cgcaggagct
    17701 gcacatcacc gtctcggcgg gcgtggcgcc gaacaagttc ctggccaaga tcgccagcga
    17761 ctggaagaag cctaacggcc tgtttgtgat taccccggat caggttgagg attttgttgc
    17821 cgccttgccg gtgagcaagc tgcatggcgt gggcaaggtc acggccgaca agctcgggcg
    17881 tttgggcatt cgtacctgta gcgacctgcg cgagtggaac aagctggcgt tggtcaaaga
    17941 attcggcagt tttggcgagc gtttgtgggg actagcgcgc gggattgatg agcgagtcgt
    18001 gcataacgac agccggcgtc agtcggtcag tgtggagaac accttcgaca aagatttgcc
    18061 agacctggcc gcctgtcttg agcagttgcc cgcgctgctg gagcaactgg ccacgcgcat
    18121 ggctcggctg gatgcgagtt atcggcccga caagcctttt gtcaaaatca agttccacga
    18181 cttcagccag actaccctgg agcagggcgg ggcgcggcgt gacctggaaa gctatacgcg
    18241 gctaatgagc gccgcctttg cccgtggcaa caaggcggtg cgcttgcttg gcgtcggtgt
    18301 gcgtttgcat gacttgcgtg gcaagcatga gcagttggag ttatttgcac cctgacagca
    18361 gtgcttagtt agcacatata aaaaaggcga agccacaggg cttcgccttt tttagtcgcg
    18421 tagggattag tgcttgcgcg gtactggttt gagcagctcg tcagacggca tttcgcaggc
    18481 gatcttgcgg cccagcagtg cttcgatggc gggtagggcg taagcgtcgt cttccccggc
    18541 aaagctgatc gaggtgccac tgctaccggc gcggccggta cgaccgatgc ggtgcacgta
    18601 gtcgtccggg gtttccggca gggtgaagtt gatcacgtga ctgatcgcgt cgacgtggat
    18661 gccgcggccg gccacgtcgg tggcgaccat cacgcggatc ttgccttcac ggaagccttc
    18721 aagggcgcgg atgcgcttgt gctgtggtac gtcgccggac atctgcacgg cgctgatgcc
    18781 gtcgcgggtc aggcgttctt cgatgcgccg cacttcatcc ttgcggttgg cgaagaccat
    18841 cacgcggatc cagtcgtttt gggtgatcag gttgtacagc agcttgtatt tgtcgctgcc
    18901 ggctacggca taaacgtgct gctcgactgt atcgctggcg acgttttccg gctcgatctc
    18961 gacgatcgca ggctcggtgg tccattgctt ggccaggttc atcacgtctt cggtgaaggt
    19021 cgcggagaac agcagagtct ggcgttcgcc cttcatcggg gtctggcgga tgatctgacg
    19081 gacctggggg ataaagccca tgtcgagcat acggtcggct tcgtcgagca ccatcacttc
    19141 gaccatgtcc aggtgcactt cgccgcgctg gttgaagtcg agcaaacggc ccggggtggc
    19201 caccagaatg tcgcagaagc gcgattcaag cagcttgagc tgtttgtcga agtccatgcc
    19261 accgacaaaa ctcatcacat taaggccggt gtatttggtc aggtcggcgg cgtccttggc
    19321 gatctgcacc accagctcgc gggtcggcgc aatgatcagc gcgcgcggtt cgcccatata
    19381 gcgctctttc ggcggcgggg tttgcagcag ctgggtgatg atcgagatca ggaacgcggc
    19441 agttttgccc gtgccggtct gggcgcggcc gatggcgtcg cgaccgctga gggtgtaacc
    19501 cagcacctgc gcctggatcg gcgtgcaata cgggaagccc agatcatgga tggcatgcat
    19561 cagctcgggg gagagcttga aatcatggaa acgggttttg ccctcggccg gtgctacggc
    19621 gaagtcttcc agtttccagg tgtcgactgg tttggcaggg cgttcgcggc gcggtttcgc
    19681 cggtttttcc ttgttctgta acggtttttc agcggcttca gtggggcgcg gtgaggcgct
    19741 gcggctggat ttgtcagcgt tggcgtcagg ctggacgcgc ggtgcgctgg gcgttacagg
    19801 tgacggagtt gctgagctgg gctgctcgcc ctcggctttg ccgaatattt tcttgagtgc
    19861 tttgagcacg gtcatctcat caattggtta aggagtgaac ggccgccagt gtaaagcaag
    19921 aagccggcgc ggcgaagtgc cgcgccagat ggcggagatt ggctggtttt tactcagttt
    19981 tcgggaaagc tggcaagcag caagcgctgc acattgccac ccatgatcgc ggcaatttcg
    20041 tcgtggctga aacccgcctt gattaggcct tcggtcagtt gcggcaggcc cgttacgtca
    20101 aacggtgcat ggatggtgcc gttgaagtcc gagcccagcg ccacgtgctg tacgccaatc
    20161 aagccggcgg tgtagcggat tgccttgacg atggcctcga ccgacgtggc gcatacggca
    20221 gcatcccagt agccgatacc gattactccg cccgtggcgg caatggcttt caggtgttgg
    20281 tcgctgaggt tgcgggtgcc tttgcacgta ccttcaacgc cggtgtggga aaccagcacc
    20341 gggcgggtgg cgatggccag tacgtcatcg atcagagggc gtgaggcgtg agctaggtcg
    20401 atgagcatgg ctttttcttc caggcgcttg atcacttgcc ggcctagcgg ggtcaggccg
    20461 cctttttcca ggccgtgggc cgagccgccg atttcgttat caaagaagtg ggtcaggccg
    20521 gtgatgcgaa aaccggcgtc atacagcgcg tcgacgtttt ccagcttgcc ctcgatcggt
    20581 tgcaggccct cggtggcgag aattgccgct acacgctgcg ggtctttctg ccaggcgctg
    20641 acaaagctgg cgaggtcgcc acggctcttg atcatgacca ggcgaccgtc gctgttggca
    20701 ctggcctcat tgagtttgtg cgcttggtag agcgcgcgtt gcagcaggct gttccaggtt
    20761 tccttgggcc agcgctgggc catggccagt agggtgatgt tgtcggtttc gccgctgttg
    20821 cgctcatagt tgatgccgcg cggcgtcttg gtcacggtgg agaatacctg cagcccaacg
    20881 cggccttcga gcatgcgcgg caggtcagag tggccgaagt cgtagcgctt gagtaggtca
    20941 cgctcccaca gcagcacgtc gtcatgcagg tcggcgataa acagctgttg atgcagctga
    21001 ttggccgatt cactggcggg gtaaggcgcg ggggattcga cgctgttcat cttgcggtca
    21061 aacagggagg gcagggcaaa cacactgcca gcagaaatca acaggactat cagcagggcg
    21121 atcagaatct tgcgcatagt ttaaggtgcc atccgggcgt tgttgttatg cgcagcactc
    21181 tagcaagagt gagtaagaaa tgtggttgat tggactgcag tacgggtgct ttaacgtttg
    21241 ttgacgatct cgcgagctag agtcctgcaa ggcaaaaaca ggcgaggaag cggagtgtac
    21301 ttttgtacat gagcattccg agtctgtttt taacgcagca gggccgacgc gcagcagatc
    21361 gtgaggatta gccgcagagg cagtcgcgac cttgctgctt ggcctgatac agcgcgctgt
    21421 cagcgcgggc gatcacgcct tgcaggctgt ccttgggttg catctgcgcc aggccaatgg
    21481 acacggtcac accgggcaac acgccaatcg gtgagtggaa cgagctgata ttgcgcagcg
    21541 ccaggcgcag ccgttcgccg atgttgcggg cctcatcaag gctgatttcc ggcagtagga
    21601 tgacgaactc ttcaccaccg tagcgggtca ggctgtcttt gggtcttagc tggctgcgca
    21661 gggtgtgcgc caccaggcac agggcataat cgccagcgag gtggccgtgt tgatcgttgt
    21721 aggctttgaa gtggtcgaca tcgagcatca gcatgcacag cggtttgcca ttgaaggcgc
    21781 tgcgggtgct ttcgcgctca aagatatgct ccagccagcg gcggttgtag gcgccggtga
    21841 gggcatcgat attggcggtc tgttcgctgt cgagaatgat ccggttgcct ttgcgcacac
    21901 ggtcgcaaag cagttcaagc atgttctgca tcaattccgg tgattgctgg aacaggttga
    21961 acagcgattc acggtgcagg cgcagcacgc agcagggctc agtcgccacg acgtaggcgg
    22021 aggggtgttc attgtcgata aagctgattt cgccggcgca atcgccgact ttcagggtgc
    22081 tgacggcctg gctctcgagc gagccgaggt agaccttgag ttggcccttg agcagcagat
    22141 agaggtactg gttgcgattg aagggcgaca gcaagacttc gccggcttcc agatcgcagg
    22201 cgcggaattc cttgagcagc tggctgatgc tgctggcggc gacgttattg aacagtcgca
    22261 ggttgcgaag ttgctgcagg tcagcctgcc atttggcgtt tttcattgga tcggcaggtg
    22321 tgactgttgt gagttgggaa tgttggtcat cgctaacgct ccctgcatta agggcgaaca
    22381 ccatgcctga acatattccg ctgcctgtgg cctgacaatc cttgtcagac gcacgggcga
    22441 ccagtggtat tgaagttgaa tggttggtca caaatctggc gcgatcctta cgccagcact
    22501 tgcttactcg ctggggtgca gttctatggc cacggcaaag ccggccaggc gtttgctcac
    22561 tgcgtcagct agcgctgctt gagctgcggg caggtagatg tgggcgacct gcccgcgctt
    22621 gtcatcgctg agcacttcca cgcgtaggcg cgaatccagc tccgccagcg cagcttggta
    22681 gacgcgcgta atggcgtcga atcgtagcgc cggcttgaag gttttaccta cggcggtcag
    22741 cgggatagcg tcgatgatcc aggcgtcctt gggaatcgcc gcacgctcgg ggatgtgttc
    22801 ggcagcatgg gccagcaatt cggcttcgct aaccttagca ccgggcttga gttgcacgta
    22861 gaccaccggc agctcgccgg ctttctcgtc aggcttgccg accgctgcgg ccatggccac
    22921 tgcggggtgc ttgtgcagcg cttcctcgat catctgcggg tcgatattgt ggccgccccg
    22981 gatgatcagg tccttgctgc gtcctgtcag ccagatatag ccatcggcat ccacgcggcc
    23041 caaatcgccg gtgttgaacc aatcgccgtc gacccagatg cctgcgttct tgctgctctg
    23101 caggtaaccc ttgaataccg tggcgccacg gatgcacaga ttgccgattt cattgcgatt
    23161 agcctccctt aaatagttgc cctgctcatc aaggatcttg atcgctactt cgcaataagg
    23221 catcggcaag ccgatggagc cggggcgacg ttcgccagca ggcgggttgg cgcagctgcc
    23281 gcaggtgcct tcggtgaggc cgtagccttc gatcagggtc agtccgattt tgctttcgaa
    23341 ctggcgaatc agctccaccg gcatcggtgc ggcgccgcac aatgcgtatt tcagcgagct
    23401 gaggtcatgg ccttcgctcg gcacctgcag caggccggcg tagatggttg gtacgccgct
    23461 aaagaagctg actttatggc gttcgatgat cgtccagaaa ttgctgatca ggttggcgtt
    23521 gcggtacccc tgcggcgtag ccagcagcac ttcggcgccg ccgatgaaag cagtcagacc
    23581 tgtaacaatc accccgttga cgtgaaacag cggcaggccg caaagggtta catcgcccgg
    23641 ctcgaagcgg gtaaccaagt tcatgctgaa cgccatggcc acttcgttgc catggctgtg
    23701 cggtgcgagc ttcggcgtac cagtggtgcc gccggtgtgg aagtagctgg caatgtcgtc
    23761 tggcgcgatt acgcggccgc tttccaggtg atcatccggg cagctggcga ttagttcatc
    23821 gaaatccagc acgccgtccg gcaatggccc gcgctgcgcc ttgatggcgg tgctctgtgg
    23881 ctcaggcagc aggttggcca tgtccacgca gataattgcc ttgagcgttg gcagttgctc
    23941 gcgcaggcct tcgaccttgg cccacaggtc ggtgccgggg aagggcgcca gggtcaccag
    24001 cagttccgag tccgaagcct gaatcagttc ggcgatatgt tccgggtcga gcagcgggtt
    24061 gatcgcgttg acgatcccgg ccgcttcgcc gccccagatg gcgaagtggg tgtgcggcag
    24121 gttaggcaac agaaacgaca ccgccttgcc cgggcgcagg cccaggcggt ggaaagcgtt
    24181 ggcggtctgg gtgatcttgc ccagcagttg cccataactg aggcggtaag ccgcctcatc
    24241 tgccgtgccc tggaggataa acgagagtgc cggtgcatcc gcttgccgcg ctgcgctgcg
    24301 gcggatcagc tcataggtac tggccggcag gtcgcgctcg gccagtggag tttgctcgat
    24361 cagctggata tcgcgcaggg tcttgatcgc agggatggca ggcataggtg ttttcgctcg
    24421 cagacagtca actggtgaac cgttgctcca gccagtcggc gatgtcgcgg atttcctcat
    24481 tcaccacttc gtggcccatc gggtaatcac gccattgcac gctgacgcca gaggcactca
    24541 ggtagtcatg agctgcgcgg cctatggccg gcagaactat gtcgtcgaat gtgccatgca
    24601 aacacaggac cggcagttgt ctttttgtat ccgccagctg gatgtcgtcg ctgaaagtcg
    24661 gcgcataggt cgacagagcg attaccccgc cgagggcgaa ctgccagcgt aggaaggcgg
    24721 tgtgcagcac caccgcgccg ccctgggaga agccggcgag aacgatgcgc gcagggtcaa
    24781 tgccgctgtc gcgttgccct tcgatcagtg cgatcacctg ctgcgctgag gcttccagct
    24841 gctcgcggtt gatcgcgcgg gccgggctca tggccagaat gtcgtaccag ctgggcatgg
    24901 cgtagccgcc gttgatcgtc actgcctgtg tcggcgcttg cggcagaacg aagcgggtgt
    24961 tgtgtaagcg cttctggagc atctccgcaa ccggcataaa gtcgtagcgg tcggcaccca
    25021 ggccgtgcaa ccaaatgacg cacgagtctg cggcaagtgt gggctgaaga atcaagggtt
    25081 cgctcatctg aggctccgct gtggtgcgcg cgcgctgatt tagtgcg
//
LOCUS       DASRUM010000071         8328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_871537, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000071 DASRUM010000000
VERSION     DASRUM010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871537"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /gene="rho"
                     /locus_tag="VFF22_04980"
     CDS             <1..987
                     /gene="rho"
                     /locus_tag="VFF22_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZX16206.1"
                     /translation="RTGDTIVGKIRPPKEGERYFALLKVDTINYDRPENAKNKILFEN
                     LTPLFPNKRLVMEAGNGSTEDITGRVIDLCAPIGKGQRGLIVAPPKAGKTIMLQNIAA
                     NIARNNPECHLIVLLIDERPEEVTEMQRTVRGEVVASTFDEPPTRHVQVAEMVIEKAK
                     RLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGVDAHALEKPKRFFGAARNIEE
                     GGSLTIIATALVETGSKMDEVIYEEFKGTGNMELPLDRRIAEKRVFPAININRAGTRR
                     EELLTADDELQRMWILRKLLHPMDEIAAIEFLTDKLKATKTNEEFFQSMKRK"
     gene            1240..2706
                     /gene="ubiD"
                     /locus_tag="VFF22_04985"
     CDS             1240..2706
                     /gene="ubiD"
                     /locus_tag="VFF22_04985"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HZX16207.1"
                     /translation="MQYRDLRDFIRGLETRGELKRIQTPVSPVLEMTEICDRTLRKGG
                     PALLFENPTGFDVPVLGNLFGTPKRVALGMGAEEVSELREIGKLLAFLKEPEPPKGLK
                     DAWNKLPIYKKVLSMAPKVLKDAPCQEVVEEGDDVDLGKLPVQTCWPGDVGPLITWGL
                     TITKGPNKERQNLGIYRQQVIGRNKVIMRWLSHRGGALDFREWCQKYPDRPYPVCVAL
                     GADPATILGAVTPVPDSLSEYAFAGLLRGHRTELVKARGSDLQVPASAEIVLEGVIHP
                     GETAPEGPYGDHTGYYNEVDTFPVFTVERITRRQKPIYHSTYTGRPPDEPAILGVALN
                     EVFVPILQKQFPEIIDFYLPPEGCSYRMAVVTIKKQYPGHAKRVMLGVWSFLRQFMYT
                     KFVIVTDDDINARDWNDVIWAITTRMDPKRDTVLIDNTPIDYLDFASPISGLGSKMGL
                     DATHKWPGETTREWGRAIVQDDAIKQRVDALWSELGID"
     gene            2706..3674
                     /locus_tag="VFF22_04990"
     CDS             2706..3674
                     /locus_tag="VFF22_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HZX16208.1"
                     /translation="MKVTLNPSGAVLDVQPQERILDAARRLGYDCPQSCRNGNCHICA
                     ALLVEGRVQQGGVELSQGELFTCLAEPLQDCVLHWDGVLAPGELLVRELACQVVACDD
                     VGGDVFRLRLQAPAGKVPRYHAGQYLLLQRDGDDYAAFSLASAPHRGRELELHVLARD
                     EATVELIAQLRRTGLVRVKMPFGDTHLAELPDGPLVLIAAGTGMAQMHSLIEHCRAAG
                     FSHPVHLYWGARRPEDFYELQHWAQWQQVPNLFLHQVVSDQCGWQGRCGLLHEAVRED
                     FSDLKALHLYASGSPAMVYATLDALVDAGMDAHQMRADVFAYAPRG"
     gene            complement(3907..4884)
                     /locus_tag="VFF22_04995"
     CDS             complement(3907..4884)
                     /locus_tag="VFF22_04995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HZX16209.1"
                     /translation="MKAVLCKAFGPAETLVLEEVASPEAKKNEILLQVHAAGVNFPDT
                     LIIEGKYQFKPPFPFSPGGEAAGVVTAVGEKVSHLKVGDRVMALTGWGSFAEEVAVPG
                     YNVMPIPASMDFASAAAFGMTYGTSMHALKQRANLQPGETLLVLGASGGVGLAAVEIG
                     KAMGAKVIAAASSAEKLEVAKAAGADELINYSESNLKDEVKRLTGGQGADVIYDPVGG
                     DLFDAAIRSIAWNGRLLVVGFASGRIPELPVNLTLLKGAAVVGVFWGSFAQRQPQDNA
                     ANFQQLFAWHAEGKLKPLVSQTFPLEQAAEAINTLGQRKAVGKVVVTVR"
     gene            5043..5459
                     /locus_tag="VFF22_05000"
     CDS             5043..5459
                     /locus_tag="VFF22_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005888059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HZX16210.1"
                     /translation="MKAWIALLLALSLPLLAQANEDKEAKEGEAATPVVAYVSLFPAL
                     VGNFGDGPKLKFFKADISLRVTGADVEAKVKHHDPLIRNQLVMLFSQQTEEAMSAPGA
                     RETLRAEALKQVQEVLTQEEGKPLVEDLLFNNLIIQ"
     gene            complement(5487..5936)
                     /locus_tag="VFF22_05005"
     CDS             complement(5487..5936)
                     /locus_tag="VFF22_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HZX16211.1"
                     /translation="MPYWLMKSEPDELSFHDLQRLEQARWDGVRNYQARNFLRSMQPG
                     DLFFFYHSSCPEPGIAGIARIASTHYPDPTALEPGSHYFDAKASADKNPWSAVDVAFV
                     EAFKRVIPLAQLKAQSALLALPLVQKGSRLSVMPVSAEEWAAVLALR"
     gene            6108..6242
                     /locus_tag="VFF22_05010"
     CDS             6108..6242
                     /locus_tag="VFF22_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16212.1"
                     /translation="MKRKPDVLLILVILFSLGVVTTGYTQSLWDRQADAPVSQSQAAG
                     "
     gene            complement(6292..6888)
                     /locus_tag="VFF22_05015"
     CDS             complement(6292..6888)
                     /locus_tag="VFF22_05015"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016978967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HZX16213.1"
                     /translation="MLDTSALSRAQLRRHLRAQRRALSRHAQRQAAQGLYKQLAQHPL
                     FRRARHIALYLPNDGEINPRPLLRAAQQRGKATYLPVLSPWPRSKMVFQRIRMGERLQ
                     KNRFGIDEPRRNCKQQRKVWALDLVLLPLVGFDHHGARLGMGGGFYDRSLAYRALRKN
                     WHKPTLLGLAHECQQVDRLAMASWDVPLQGTVTDKAWY"
     gene            complement(6938..7116)
                     /gene="ssrS"
                     /locus_tag="VFF22_05020"
     ncRNA           complement(6938..7116)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFF22_05020"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(7150..7464)
                     /locus_tag="VFF22_05025"
     CDS             complement(7150..7464)
                     /locus_tag="VFF22_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HZX16214.1"
                     /translation="MSQPDTVTVQILDKEYCIACPTNQRANLESAARYLDGKMREIRS
                     SGKVIGADRVAVMAALNITHDLLHKQQSLDQEVSSTREQVRDLLDRVDHALADDPDSR
                     NG"
     gene            complement(7467..7673)
                     /locus_tag="VFF22_05030"
     CDS             complement(7467..7673)
                     /locus_tag="VFF22_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HZX16215.1"
                     /translation="MEDANLHTLTVKFELLIRRVEQLKADKRVLLANEKAWREERAQL
                     IEKNEMARHKVESMISRLKALEQD"
     gene            7831..>8328
                     /locus_tag="VFF22_05035"
     CDS             7831..>8328
                     /locus_tag="VFF22_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017902651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HZX16216.1"
                     /translation="MPIQNSPYSAFSALLASSGQPVSPAELHGLLLGRSCAGAGFESE
                     PWLVDAAELLGGAPSDGVHQALIGLQAMVKDELSGTDMAMVLLLPSDDAPLAERAAAL
                     GQWCQGFLGGFGLTARETALSAEASEVLQDLAAIAQVQSALEESEDAEGDYMEVMEYL
                     RVATLL"
BASE COUNT         1572 a         2475 c         2600 g         1681 t
ORIGIN      
        1 cgtactggcg ataccatcgt cggcaagatt cgcccgccga aagaaggcga gcgttacttc
       61 gcgctgctca aggttgacac gatcaactac gaccgcccgg aaaacgccaa gaacaagatt
      121 ctgttcgaaa acctgacgcc gttgttcccc aacaagcgtc tggtgatgga agccggtaac
      181 ggctccaccg aggacatcac tggccgggtg atcgacctct gcgccccgat tggcaaaggc
      241 cagcgtggcc tgatagtcgc accgccgaaa gccggtaaga ccatcatgct gcagaatatc
      301 gcggcgaaca tcgcgcgcaa caaccccgag tgccacctga tcgttctact gatcgacgag
      361 cgcccggaag aagtgaccga aatgcagcgc accgtgcgcg gcgaagtggt tgcctcgacc
      421 ttcgacgagc cgccaacccg ccacgtgcag gttgccgaaa tggtcatcga gaaggccaag
      481 cgcctggtcg agcacaagaa ggacgtggtc atcctgctcg actccatcac ccgtctggcg
      541 cgtgcctaca acaccgtgat cccaagctcc ggcaaggtgc tcaccggtgg tgtcgacgct
      601 catgccctgg agaagccgaa acgcttcttc ggcgccgcgc ggaacatcga ggaaggcggt
      661 tcgctgacca tcatcgccac cgcgctggtc gaaaccggct cgaagatgga tgaagtgatc
      721 tacgaagagt tcaaaggcac cggtaacatg gagctgccgc tggaccgtcg catcgctgaa
      781 aagcgtgtgt tccctgcgat caacatcaac cgcgccggta cccgccgcga agaactgctg
      841 accgccgatg acgaactgca gcgcatgtgg attctgcgca agctgttgca cccgatggat
      901 gagattgccg ccatcgagtt cctcaccgac aagttgaaag cgaccaagac caacgaagag
      961 ttcttccagt cgatgaagcg taagtaagag gatctactgc gcatcggtga tgctgcgtta
     1021 aaaacagact caaaatgctc atgtacaaaa gtacactccg ctttttcgtc tgtttttgcc
     1081 ttgcctgacc ttcgctcgct acgatcctaa acatacgcac ctacaaaagc cgggtaagcc
     1141 cggcttttgt gttcctgggt attggctttc ccttctgaaa gacccagttc ggcgctaaac
     1201 tttgcgcccc tattgctcgg cccgaaacga ggcatctgca tgcagtatcg tgacctgcgc
     1261 gactttattc gcggcctgga gacgcgcggc gaactcaagc gcatccagac gccggtttcc
     1321 cctgtgctgg aaatgaccga aatctgcgac cgcaccctgc gtaaaggcgg gccggcgctg
     1381 ctcttcgaaa accccacagg cttcgacgtg ccggtactcg gcaacttgtt cggcacgcca
     1441 aagcgcgtgg ccttgggcat gggcgcggaa gaagtcagcg agctgcgcga aatcggcaag
     1501 ctgctggcct ttctcaagga acctgagccg ccgaaaggcc tcaaggacgc ctggaacaag
     1561 ctgccgatct acaagaaggt gctgagcatg gcgcccaagg tgctgaagga tgcgccgtgt
     1621 caggaagtgg tcgaagaggg cgacgatgtc gacctcggca agctacccgt gcaaacctgc
     1681 tggccaggcg atgtcggtcc gctgattacc tggggcctga ccatcaccaa aggcccgaat
     1741 aaagagcggc agaacctcgg tatctaccgc cagcaagtga tcggccgtaa caaggtgatc
     1801 atgcgctggc tcagccaccg tggcggcgcc ctggatttcc gcgagtggtg ccagaagtac
     1861 ccggatcgtc cctatccggt atgcgtcgcc cttggcgccg atccggcgac tattctcggc
     1921 gcggtcaccc cggtgcccga cagcctttcc gaatacgcat ttgccggcct gctgcgcggt
     1981 caccgcaccg agctggtcaa ggcccgtggc agtgatctgc aggtgccggc cagtgctgaa
     2041 atcgtcctgg agggtgtaat ccacccgggc gagaccgcgc cggaaggccc atacggcgac
     2101 cacaccggct attacaacga ggttgatacc tttccggtgt ttaccgtcga gcgcattacc
     2161 cgtcggcaaa agccgatcta tcacagcacc tacaccggtc gtccgccgga tgagccggcg
     2221 attctcggcg tggcgctcaa cgaagtgttt gtgccgatcc tacagaagca gtttcccgag
     2281 atcatcgact tctacctgcc gccggaaggc tgttcctacc gcatggcggt ggtgaccatc
     2341 aagaagcagt accctggcca cgccaagcgg gtaatgctcg gtgtgtggtc gttcctgcga
     2401 cagttcatgt acaccaagtt cgttatcgtc accgacgacg atatcaacgc aagggactgg
     2461 aacgacgtga tctgggcaat taccacgcgc atggacccca agcgcgacac cgtgctgatc
     2521 gataacacac cgatcgatta cctcgacttt gcctcgccga tctcaggttt gggctcgaag
     2581 atggggctgg atgccaccca caagtggccg ggcgagacga cgcgcgagtg gggtcgggct
     2641 atcgttcagg atgatgcgat caagcagcgc gtcgacgcgc tgtggtctga gttgggaatt
     2701 gattgatgaa agtcaccctg aacccttcag gcgcggtact tgacgtgcag ccccaggaac
     2761 gcatcctcga tgcggcacgg cggcttgggt atgactgccc gcagagctgc cgcaatggca
     2821 actgccatat ctgcgcggcg ctgctggttg aaggccgtgt gcagcagggc ggggttgagc
     2881 tgagtcaggg tgagctgttc acctgtctgg ccgaaccgct gcaagactgt gtgctgcact
     2941 gggatggcgt gctggcccct ggcgagctgc tggtgcgtga gctggcctgc caggtggtag
     3001 cgtgcgatga cgtaggtggc gatgtctttc gtctgcgcct gcaggcgcct gccggcaagg
     3061 tgccgcgtta tcacgccggg caatacctgc tgctgcagcg cgatggtgac gattacgcgg
     3121 cgttctccct ggcctcggca ccgcatcgcg gacgcgagct ggagctgcat gtactggctc
     3181 gcgacgaagc cactgtcgag ctgattgccc agttgcgtcg taccggcctg gtgcgagtga
     3241 aaatgccttt cggtgatact cacctggccg agctgccgga tggaccactg gtgttgatcg
     3301 ccgccggtac cggcatggcg cagatgcaca gcctgattga acactgccgc gccgccggtt
     3361 tctcccaccc ggtgcatctg tactggggcg cgcgccgtcc agaagacttc tacgaactgc
     3421 agcactgggc gcagtggcag caggtgccca atctgttcct gcatcaggtg gtcagtgatc
     3481 agtgcggctg gcaaggtcgt tgcggcttat tgcacgaggc tgtgcgcgaa gacttcagtg
     3541 acctcaaggc gctgcacctc tatgccagcg gctcgccggc gatggtctac gccaccctgg
     3601 atgcgctggt ggatgccggt atggatgcgc accagatgcg cgcggatgtc tttgcctacg
     3661 cgccgcgcgg ctgaatcccg cgcataaaaa aggccgcctg atggacgtaa tgctgttcac
     3721 ttaagcatcg ggtggacgcg atcatcccct cgcccctccg gggagagggg aaggcctcca
     3781 gtttggcggt gttttcagcc ctcaccccca gcccctctcc cataaatggg agaggggagt
     3841 aaaaagcatg cgacctaaag tgaacagcat tacgcctgat ggacggcctt ttgctgagcg
     3901 tttggcttaa cgcacggtga ctaccacttt gcccaccgcc ttgcgctggc cgagggtgtt
     3961 gatcgcctcc gccgcttgtt ccagtgggaa ggtttgcgac accagcggct tgagcttgcc
     4021 ttcggcatgc caggcgaaca gttgctggaa gttggcggcg ttgtcctgcg gctggcgctg
     4081 ggcgaaggag ccccagaata ccccgaccac ggcggcgccc ttgagcaggg tgaggttgac
     4141 cggcagctcg ggaatgcgcc cgctggcaaa acccactacc agcaggcggc cgttccaggc
     4201 gattgagcga atggcggcat cgaacaagtc gccacccacc gggtcataga tcacgtctgc
     4261 gccctgtccg ccggtcaggc gtttcacttc gtccttcaga ttgctttcgc tgtagttgat
     4321 cagctcatcg gcgccggcgg ccttggctac ttcgagtttt tccgcgctgc ttgcagctgc
     4381 aatcacctta gcgcccatgg ccttgccgat ttccactgcc gccaggccaa cgccgcccga
     4441 tgcgccgagc accagcaggg tttcccccgg ttgcaggttg gcgcgctgct tgagggcgtg
     4501 catcgaagtg ccgtaggtca tgccgaaggc ggcagcgctg gcaaagtcca tgctcgccgg
     4561 gatcggcata acgttgtagc cgggcaccgc cacttcttcg gcaaagctgc cccagccggt
     4621 cagggccatc acccggtcgc cgaccttcag gtggctgact ttctcgccca cggcagttac
     4681 cacgccggcg gcttcaccgc ctggggaaaa ggggaagggc ggtttgaact ggtatttgcc
     4741 ttcgatgatc agggtgtcgg ggaagttgac cccggccgca tggacctgga gcagtatttc
     4801 gtttttcttc gcttcggggc tggcgacttc ttccagtacc agggtttcgg cggggccgaa
     4861 ggctttgcac agcacggctt tcatcgggct tttcctctgc ggttgatggc catgcagtgt
     4921 aggtgcgggc ggtgcccggt caatgagcat gcctgtgcct gatggtcatg cataagcttg
     4981 gggcttgggc cgctggctca ctgtggctat gctaggggct gaactcttga ggaataccgc
     5041 ctgtgaaagc ctggatcgca ttgctgttag ccctgtccct gccattgctg gcgcaggcca
     5101 atgaggataa agaggctaaa gagggagagg cagccactcc ggtagtggct tatgtttctc
     5161 tatttcctgc gctggtgggg aattttggtg atgggccgaa gctcaagttc ttcaaggccg
     5221 atatttcctt gcgggtaacc ggtgcggatg tcgaggccaa ggtcaaacac cacgatccac
     5281 tgatccgcaa tcagttggtc atgctgttct ctcagcagac tgaagaggcc atgtcagccc
     5341 caggcgctcg cgaaaccttg cgtgccgaag cgctcaagca ggtgcaggaa gtgctcaccc
     5401 aggaagaggg caagccactg gtcgaagatc tgctattcaa taacctgatt attcaataac
     5461 cctcatgcag caggcgctgc gccatttcag cgcagtgcca gcacggctgc ccactcctcg
     5521 gcgctgaccg gcatcaccga gaggcggctg cccttctgta ccagcggcaa cgctagcagc
     5581 gcactctgcg ctttcagctg cgccagcggg atcacccgct tgaaggcctc gacaaacgcc
     5641 acatcgaccg cgctccaggg attcttgtct gcgctggcct tggcatcgaa gtagtggctg
     5701 ccaggctcta gcgccgtagg atcggggtag tgcgtcgagg cgatgcgggc aattccggca
     5761 atgcctggct ccgggcaact ggagtggtaa aagaagaaca gatcccccgg ctgcatgctg
     5821 cgcaggaagt tgcgcgcctg gtaattacgc acgccatccc agcgcgcctg ctccaggcgc
     5881 tgcagatcgt ggaacgagag ttcgtcgggt tctgacttca ttaaccaata aggcatgaac
     5941 tttgctccta tgattcagtc cgttagcggg caggtgctgt ttacctggtg gtgcccggtg
     6001 tgtagcagtg ttgcacccgg catgtgtgga tggcagccgc tgttggacct ataaaaataa
     6061 tcgccgcgaa tacagcgtgc gctatgcctt taagggggag gcattcgatg aaacgtaaac
     6121 cagatgtgtt gttgattttg gtaattctgt tcagcctggg agtggtaact accggttata
     6181 cccagagctt gtgggatcgc caggctgatg cgccggtcag tcagtcgcaa gcggctggtt
     6241 gaacctggta cgtaccgcag ggagtgcggt acagccttta aacggcttga ttcaatacca
     6301 cgccttatcc gtcactgtgc cttgcagtgg cacatcccag ctggccatcg ccaggcgatc
     6361 cacctgctga cattcatgcg ccaaacccag taatgtcggt ttgtgccaat ttttgcgcaa
     6421 tgcgcgatac gccaagctgc gatcataaaa cccgccgccc atgcccagac gcgcgccgtg
     6481 gtggtcgaaa ccgactagcg gtagcaatac cagatccagt gcccagacct tgcgttgctg
     6541 cttgcagttg cgccggggct cgtcaatgcc gaagcgattc ttctgcaggc gctcacccat
     6601 acggatgcgt tgaaacacca tcttgctgcg cggccagggg ctgagcaccg gcagataggt
     6661 cgccttgccc cgttgctggg cggcgcgcag cagggggcgc ggattgatct cgccgtcatt
     6721 cggcaggtac agggcaatat gccgcgcgcg gcgaaacagc gggtgttgtg ccagctgttt
     6781 atacaaacct tgggcggctt ggcgttgagc gtggcggctg agggcgcgac gctgcgcacg
     6841 cagatggcgg cgtaattgcg cgcggcttag tgcagaggta tcgagcatgg gctgttgctg
     6901 tagcgatcgc cgcagaaggg caaccggcta gaaagaatga ctccccgacg tgccgctgtc
     6961 ggcttagccc ttgaacccga aagttcaagg tggaggttgc aggaggcgtt aaggctttcc
     7021 gtcaggcgga catgcacacc ggcctcaact tgcaaccccc gggtttgtgc gtatcggctc
     7081 agggacatca ccaactggcg cacatatcag ggagtagcgg ccagtatacc tgaatccgcg
     7141 tggcggtcat cagccgttac ggctatcggg atcgtcggcc agggcatggt cgacccgatc
     7201 gagcaggtcg cgcacctgct cgcgggtgga gctgacttcc tgatccaggg actgctgctt
     7261 gtgcagcagg tcatgggtga tattcagcgc ggccatcacg gcgacgcggt cggcgcctat
     7321 gaccttgccg ctggagcgga tctcgcgcat cttgccgtcc aggtagcgcg cggcgctttc
     7381 caggttggcg cgctggttgg tgggacaggc gatgcagtat tccttgtcga ggatctgcac
     7441 ggtaacggta tcgggttggc tcatgatcag tcctgctcca gggctttaag gcgcgaaatc
     7501 atcgattcga ccttgtgccg ggccatttcg ttcttctcga tcagttgggc gcgttcctcg
     7561 cgccaggcct tttcattcgc cagcagcaca cgcttgtcgg ccttgagctg ctcgacgcgt
     7621 cggatcagca attcgaactt gacggtcagt gtgtgcaggt tggcgtcttc catggtctct
     7681 caccagggtt gggatgggcg caactatata ggattgcccc aggtaagggg caaaccggac
     7741 ggcggcctgg tgatggcctc ggcgcgcctg ccggtgctag gatacgaggc cttcattcta
     7801 gtcattgcgc cctgtggcgc ctagtcgcct atgcccatcc agaattcgcc ctacagtgct
     7861 ttttccgccc tgctcgccag cagcggtcaa cccgtttcgc ccgccgaatt gcacggcctg
     7921 ttgctggggc gcagttgcgc cggtgccggc ttcgagtccg agccctggct ggtggatgcc
     7981 gccgagctgc tgggcggcgc gccctcggat ggagtgcacc aggccctgat cggcctgcag
     8041 gccatggtca aggatgaact gtccggcacc gacatggcca tggtgttgct gttgccatcc
     8101 gacgatgcgc cactggccga acgggccgcg gccctggggc agtggtgcca gggttttctc
     8161 ggcggcttcg gtctgaccgc ccgcgagacg gccctgagtg ccgaggcgag cgaggtgctg
     8221 caggacctgg cggccatcgc ccaggtacag agtgccctgg aggaatccga ggacgcggaa
     8281 ggtgactaca tggaggtgat ggagtacctg cgggtcgcca cgctgttg
//
LOCUS       DASRUM010000072        21407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1712888, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000072 DASRUM010000000
VERSION     DASRUM010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 21407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21407
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712888"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VFF22_05040"
     CDS             <1..893
                     /locus_tag="VFF22_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX16217.1"
                     /translation="DSRLSDFLLRAEAVLARLEPLLPAQRPVIDWQQSLAARWQRDGR
                     NGFLAPLQVSLDVSLDDLIGVDRQRAQLARNTQQFIQGLPANHALLWGARGTGKSSLV
                     RALLAEHAAAGLRLIEIERDHLADLPRIVEQLSELPQRFVLFCDDLSFEAGEGDYRML
                     KTVLDGSLERAPDNVLLYATSNRRHLLPEKHSDNESWQRVDGELHPNEAVEDKIALSD
                     RFGLWLSFYPFTQEHFLDVVRHWVEVHAAQAGLAWQWHDELEKAAIRWALGRGNRNGR
                     CAYQFARDWVGLQLLSKTDD"
     gene            886..1368
                     /locus_tag="VFF22_05045"
     CDS             886..1368
                     /locus_tag="VFF22_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZX16218.1"
                     /translation="MIDLQQSGQGLDGYPLLVAQLQALLAGERDFIANAAQFSAFLFQ
                     ELSDLNWAGFYLARGEELVLGPFQGKVACVRIPFGRGVCGAAAATGQTQRVEDVHAFA
                     GHIACDSASNSELVVPLFKDGRLLGVLDLDSPSLARFSAADQAGVERLVEVFVALTDC
                     "
     gene            complement(1429..2091)
                     /locus_tag="VFF22_05050"
     CDS             complement(1429..2091)
                     /locus_tag="VFF22_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017149142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZX16219.1"
                     /translation="MSLTVYGAPLSPFVRKVRLFLAEKNLDYQLEIILPFGQPAWYRD
                     MSPLGRIPAMKDGDFSLADSSVICQYLDDKHPDQSNLLGQNAEQRAQVRWLEKYADYE
                     LAPLCTFTVFRNRALKPSMGQGCDEAAVQKALHEKLPAHFDYLEKTLGSAQYLVADSL
                     TLADLALACQLINMQHGDEQLDAQRWPNLSAHYARITARDSVQAVLPGELKMLAKMAA
                     KA"
     gene            complement(2143..2913)
                     /locus_tag="VFF22_05055"
     CDS             complement(2143..2913)
                     /locus_tag="VFF22_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010564961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16220.1"
                     /translation="MSPLVITILIVSGIAILIAIAYINHMVENNKLEKARIKADLNDR
                     LRRCTDLAEALPGQLMTPALKLLLSRLQLHFSERMLPLDKGNNALKTRIEELRKLIAQ
                     GESIPVNNPPQPILNEAKAKEVRYQLENLHGQLTRCAKNGIIPTNEAKHWLKEVRHML
                     TQVHIEFFGNLGQQALQQNQPGQARLAFERGVQYLRKQPDPAPYQNALKKFEEQLARA
                     NAMVLESNKPTTDQATELDAGLESLGGDDEWKKKNIYD"
     gene            3135..3210
                     /locus_tag="VFF22_05060"
     tRNA            3135..3210
                     /locus_tag="VFF22_05060"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3169..3171,aa:Glu,seq:ttc)
     gene            3249..3324
                     /locus_tag="VFF22_05065"
     tRNA            3249..3324
                     /locus_tag="VFF22_05065"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3282..3284,aa:Gly,seq:gcc)
     gene            3368..3443
                     /locus_tag="VFF22_05070"
     tRNA            3368..3443
                     /locus_tag="VFF22_05070"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3402..3404,aa:Glu,seq:ttc)
     gene            3465..3540
                     /locus_tag="VFF22_05075"
     tRNA            3465..3540
                     /locus_tag="VFF22_05075"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3498..3500,aa:Gly,seq:gcc)
     gene            complement(3667..4077)
                     /locus_tag="VFF22_05080"
     CDS             complement(3667..4077)
                     /locus_tag="VFF22_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2817 family protein"
                     /protein_id="HZX16221.1"
                     /translation="MANTHIDHHLALLQHLRTILVALGEAEQILDDSHAMYLERYDEL
                     LSDLPGDPEASLYLGQDLISQIFQRYPQIAHLVPRDLLWFFGGDCLHFMPDEEIEMYQ
                     TLEERRFYAEENDEPFDWNQEKQLLAMPAQGSKH"
     gene            complement(4144..6591)
                     /locus_tag="VFF22_05085"
     CDS             complement(4144..6591)
                     /locus_tag="VFF22_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZX16222.1"
                     /translation="MLALWLLALLLGTAFLAHRRTAPLPAVGIVAGFLILMGVFSHAP
                     AWLMLVLWLLLLAAALPLLLPQQRRSLLTAPLFAWFKRVLPPMSATERDAIEAGTVWW
                     DGELFSGRPDWDTLLAYPKAQLTEEEQAFIDGPTEELCAMVSDWQIGQQMDLPAEAWA
                     HIKSHGFFALIIPKQYGGKGFSAYAHSQVAMKLATRSGDLASTVMVPNSLGPAELLLH
                     YGTDAQRDHYLPRLARGEDIPCFALTGPLAGSDAGAMPDTGIICKGQWQGEEVIGLRL
                     NWEKRYITLGPVATLLGLAFKAYDPEHLLGEEEDLGISLALVPTETPGVEIGRRHLPL
                     GAAFMNGPNSGKDVFIPLEYLIGGQEMLGKGWMMLMNCLSVGRSISLPAVGTGAAKFT
                     SLTTGQYSQVREQFNVPLAAFEGIQEALARIGGNAWLMDSARILTANAVDLGEKPSVL
                     SAILKYHLTERGRECISHAMDVHGGKGIVMGPNNYLARSWQGAPIFITVEGANILSRN
                     LMIFGQGAIRCHPYVLKEMALASHADQDQALQEFDELLLQHIGFAVSNAASSLLLSLG
                     FGVFSQVPGDRISRPYFRALNRLAASFALLADTSMMLLGGELKRRERLSARLGDVLSH
                     LYLASAALKRYHDLGYPEHSQPMLRWAVEESLGKAEQALEELLSNFPSKLLGCALRLL
                     VLPLGRRHKGPSDELDAEVAGIIGRNSGDPALEELLEGCYRPQAEQDPVGALQYAMNL
                     LQDVQPLQKKLHKAVKAGQVQELPGQSLIDAALAAGTLNPEEAHSLHQAEAARRVVID
                     VDDFAKEELTLGSGKVR"
     gene            6745..7404
                     /locus_tag="VFF22_05090"
     CDS             6745..7404
                     /locus_tag="VFF22_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZX16223.1"
                     /translation="MREYLEPGRFIDSDHPALVEFAERFRGASRDPREQAIQLYYAVR
                     DEIRYNPYVFSRDADTLKASHALQAGESYCVPKATLLAACARHCGIPARIGLADVRNH
                     LATPRLLEMLRSEVFAMHGYTELYLDGRWVKATPAFNLALCRVFKVAPLEFDGLSDSV
                     FHPFNQQGERYMEYLHDHGQFADVPEQLFFGHLAACYPHLFAEDALSISGDFAAEAVP
                     G"
     gene            7616..8548
                     /locus_tag="VFF22_05095"
     CDS             7616..8548
                     /locus_tag="VFF22_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017905784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX16224.1"
                     /translation="MSTALSIRQLTKTYGNGFQALHGIDLDVAEGDFFALLGPNGAGK
                     STTIGILSTLVTKSSGTVNVFGHDLDKQPSALKRCLGVVPQEFNFNQFEKAFDIVVTQ
                     AGYYGIPAKIAKERAEQYLNQLGLWGKHNVPSRELSGGMKRRLMIARALVHQPRLLIL
                     DEPTAGVDIELRRSMWSFLTELNQQGITIILTTHYLEEAEQLCRNIGIIDHGRIVENT
                     SMKDLLKKLHVETFLLDLKESLLVAPQLIGYPAQLLDGHTLEVQVDKAQGITELFRQL
                     ALQNVEVLSLRNKTNRLEELFVSLVEKNLAKVAV"
     gene            8560..9339
                     /locus_tag="VFF22_05100"
     CDS             8560..9339
                     /locus_tag="VFF22_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX16225.1"
                     /translation="MNSEFTANMVALRTIVYREIRRFTRIWPQTLLPPAITMVLYFVI
                     FGNLIGSQIGDMGGFTYMEYIVPGLIMMSVITNSYGNVVSSFFGSKFQRSIEELMVSP
                     VSPHIILTGYVVGGVLRGLAVGFIVTLLSLFFTKLQVHHLGVTVLVVLLTATIFSLGG
                     FINAVFARNFDDISIIPTFVLTPLTYLGGVFYSITLLPQFWQTVSLANPVLHMVNAFR
                     YGILGVSDIRIGVAIGFMLVATAVLYGVCIRLLVSGRGMRQ"
     gene            9430..10461
                     /locus_tag="VFF22_05105"
     CDS             9430..10461
                     /locus_tag="VFF22_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZX16226.1"
                     /translation="MFARAPALLGTVFLLCSLVSVQAHANEGPLVEVVKAERSLVRDE
                     LVTFGSLRPNESVMIRPEIAGRVAQLHFREGQRVEAGALLVSLDDSITRAEFAQARAN
                     LNLAEKNFERAQMLFKRGASNAQALDEASAQQQASRASLALSQARLDKTRILAPYDGV
                     LGLRAVSPGDYLSEGQDIVNLEVLDPLKVDFRIPQKAVSQIRLKQVIEISLDAYPDER
                     FTGEIFAINPRLDEAGRSQAIRAHISNSDGRLSPGQFVRVSVILAERPNALVIPEEAI
                     MPVGEKLLVNLVVDGKVELREVKLGKRLAGKVEVLEGLQGDETLISAGWQKVRAGAPV
                     RTKVVEPAP"
     gene            10458..13525
                     /locus_tag="VFF22_05110"
                     /pseudo
     CDS             10458..13525
                     /locus_tag="VFF22_05110"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(13596..14603)
                     /locus_tag="VFF22_05115"
     CDS             complement(13596..14603)
                     /locus_tag="VFF22_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZX16227.1"
                     /translation="MNAPLNRQFLLAQRPVGLPSRETFSYVENSVGEPGPGQILVKNA
                     YLSLDPAMRGWMNDAKSYIPPVGIGEVMRALGVGEVVASQHPDFAVGNHVNGALGVQD
                     YFLGEPKGFYKVDPKRAPLPLYLSALGMTGMTAYFALLDVGAPQAGETVVISGAAGAV
                     GSIAGQIAKLKGCRVVGIAGGADKCKFLIDELGFDGAIDYKSEDVIAGLKRECPKGVN
                     VYFDNVGGDILDAVLTRLAFKARVIICGAISQYNNKQAVKGPANYLNLLVNSARMEGM
                     VVMTYAPRFAEAAAEMGGWMASGKLKSKEDIVSGLQTFPETLLKLFSGENFGKLVLKI
                     D"
     gene            14828..15589
                     /locus_tag="VFF22_05120"
     CDS             14828..15589
                     /locus_tag="VFF22_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX16228.1"
                     /translation="MSFSFSGRVALVTGGAAGIGRATALAFANEGLQVVVSDVDVAGG
                     ETTVALIREAGGDALFVRCDVTRDAEVKVLMERTLAAYGRLDYAFNNAGIEIEKGKLA
                     EGSESEFDAIMGVNVKGVWLCMKHQIPLLLAQGGGAIVNTASIAGLGAAPKMSIYAAS
                     KHAVIGLTKSAAVEYAKKKVRVNAVCPAVIDTDMFRRAYESDPKKGEFAAAMHPVGRI
                     GKVEEIATAVLYLCCDNAAFTTGQALAVDGGATAI"
     gene            15736..16620
                     /locus_tag="VFF22_05125"
     CDS             15736..16620
                     /locus_tag="VFF22_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HZX16229.1"
                     /translation="MTADPLLTLFLPIALGIIMLGLGLSLTLADFARVVKFPKPVLIG
                     LSCQLLLLPVVCFFLAKAFGLAPALAVGLMLLAASPGGTTANLYSHLAHGDVALNITL
                     TAVNSVIAVLTMPLIVNLSLAYFMEGDQALPLQFTKVVQVFVIVLGPVAIGIWIRSRF
                     PGFAERMEKPVKIISALFLLIIILLAVVKDWQTFVDYAPLVGGAALAFNLISLAVGYW
                     VPRLCKLSLRQSIAIGMEIGIHNGTLAIALALSPMLLNNATMSIPAAIYSIIMFFTAA
                     AFGWWVNFAHGKELKTTE"
     gene            complement(16680..17654)
                     /gene="cysB"
                     /locus_tag="VFF22_05130"
     CDS             complement(16680..17654)
                     /gene="cysB"
                     /locus_tag="VFF22_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HZX16230.1"
                     /translation="MKLQQLRYIWEVAHHDLNVSATAQSLYTSQPGISKQIRLLEDEL
                     GVEVFARSGKHLTRITPAGERIINTAGEILRKVESIKQIAQEFSNEKKGTLSIATTHT
                     QARYALPPVISAFIKQYPDVALHMHQGTPMQIAEMAADGTVDFAIATEGLELFNDLIM
                     MPCYRWNRCVVVPQGHPLTKLPKLTLEALAEYSIVTYVFGFTGRSKLDEAFSHRGLTP
                     KVVFTAADADVIKTYVRLGLGVGIVAKMAVDPKLDPDLVVLDAGELFEASVTKIGFRR
                     GTFLRGFMCDFIEKFAPHLTRDMMAKAVQCHSKVELEELFEGVELPLH"
     gene            complement(17767..18267)
                     /locus_tag="VFF22_05135"
     CDS             complement(17767..18267)
                     /locus_tag="VFF22_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZX16231.1"
                     /translation="MIRSILYATDLGLYAPYILQHALALTRSFNASLYVVHAVEPMGL
                     FAESVLQTYLDEERLKDLRTNGLSTVMASIEQRVLEGFRDEIGEALHDLELIKAVRVI
                     QGDPPLVILDEAQQLGVDLLVLGSHSHGTDMDIPLGRTASRLVQLSEVPVYLVPMLQH
                     RSRGDL"
     gene            complement(18418..19323)
                     /locus_tag="VFF22_05140"
     CDS             complement(18418..19323)
                     /locus_tag="VFF22_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HZX16232.1"
                     /translation="MSKGLGDKLVLAISSRALFDLSESHRIYESQGVAAYRQYQIEHE
                     DEILSPGDAFPLVEKLLALNTALEQQRVEVILVSRNSADTGLRAFNSIQHYGLGISRA
                     AFVGGRSPDPYLAAFGCHLFLSTHAEDVRSALRAGFGAATILSGGARRAASNELRIAF
                     DGDAVLFSDESERVYQQAGLEAFQSHERESARELLGGGPFKPFLAALHRLQQEFPEEQ
                     CPIRTALVTARSAPAHERVIRTLREWNIRLDESYFLGGLEKAAILETFGADVFFDDQA
                     GHCEKAREVVATGHVPHGISNEPQL"
     gene            complement(19333..20280)
                     /locus_tag="VFF22_05145"
     CDS             complement(19333..20280)
                     /locus_tag="VFF22_05145"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003152905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-dehydropantoate 2-reductase"
                     /protein_id="HZX16233.1"
                     /translation="MNPSPRIGIIGSGAIGGFYGLMLARAGFDVHFLLRSEFAAVASQ
                     GLQVNSAVHGNLHLDNVQAYQSAADMPPCDWLLIGAKTTSNAELAPLISQAAAPGAKV
                     VLMQNGLAVEDELRPLLPDSLHLLGGLCYICAHRSAPGVVEHQALGSVNLGYHSGPAS
                     DADSRLQILEQGSALFQAAGLDSTAMAELNQARWQKLVWNVPYNGLAVLLNSATTALM
                     GNGDSRTLIQAIMQEVVDAASACGYQLPAGFAGKLLAATDRMPDYLPSMYHDFALQRP
                     LELHAIYAAPLAAAAQAGCAMPRTEMLYQTLRFLEQRTQ"
     gene            complement(20280..20606)
                     /locus_tag="VFF22_05150"
     CDS             complement(20280..20606)
                     /locus_tag="VFF22_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZX16234.1"
                     /translation="MELTDLDADRHLLDLPGTSLLIFTSIGCASCRWARRELPKLPLP
                     IQRLAWVDAANSGGLVTRYEVFHLPALFVVQDGQFYGALQSRLQLAELTQALDTALSR
                     PAEELP"
     gene            20665..21084
                     /locus_tag="VFF22_05155"
     CDS             20665..21084
                     /locus_tag="VFF22_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZX16235.1"
                     /translation="MRHFLRHPSDMPVELVLRKQAFVPKERLNNISLGGVACNSNRAF
                     RRGTAIELRIPLFGEHARYPGLVAWCRKQEGKYLVGIAFVDEDTLFRARMVEQVCQIE
                     HYRHQREQELGCSLPVEVVAQEWISRHAAEFSRASLN"
BASE COUNT         3958 a         6120 c         6768 g         4561 t
ORIGIN      
        1 tggattctcg tttaagtgat tttttgttgc gcgcagaggc tgtattggcg cgcctggagc
       61 cgttactgcc ggcccagcgc ccggttatcg actggcagca gagcctggcg gcacgttggc
      121 aacgtgatgg tcgcaacggc tttctggcgc cgctccaggt cagtctggat gtgagtctgg
      181 atgatctgat cggggtagac cgtcagcgtg ctcagttggc gcgcaatacc cagcagttca
      241 ttcagggcct gccggccaac cacgcattgc tctggggcgc gcgcggcact ggtaaatcat
      301 ccttggtgcg tgcgctgttg gccgagcatg cggcggcggg gctgcgtctg atcgagatcg
      361 aacgtgatca cctggccgat ctgccgcgca tcgtcgagca gttgagcgag ctgccgcagc
      421 gttttgtgct gttctgcgat gacttgtcat tcgaggctgg cgagggcgat taccgcatgc
      481 tgaaaaccgt gctggatggt tctctggagc gtgcgccgga caatgtgttg ctgtatgcca
      541 cctccaaccg ccgtcatttg ctgccggaaa aacacagcga caacgaaagc tggcagcgtg
      601 tggatggcga gctgcacccc aacgaggcgg tggaagacaa gatcgcgttg tccgatcgtt
      661 tcggcctgtg gctgtcgttt tatcccttta ctcaggagca cttcctcgac gtggtaaggc
      721 actgggtcga ggtgcatgcc gcgcaggccg gtttggcctg gcaatggcac gacgagctgg
      781 aaaaggcggc aatacgctgg gccttggggc gcggtaaccg taatggccgc tgcgcttatc
      841 aattcgcccg tgattgggta ggtttgcaat tactgagtaa aactgatgat tgatctacaa
      901 caaagcgggc aggggctcga cggttacccg ttgctggtgg cgcaactgca ggcgctgctg
      961 gcgggtgagc gcgattttat tgctaatgcg gcgcagtttt cggcttttct gtttcaggaa
     1021 ctgagcgacc tgaactgggc gggtttctac ctggctcgcg gcgaggagct ggtgctcggg
     1081 ccgttccagg gcaaagtggc ctgtgtgcgg attcccttcg gtcgcggcgt atgtggtgct
     1141 gcagcggcca ctgggcagac tcagcgcgtt gaagatgtac atgcatttgc cgggcatatt
     1201 gcctgcgaca gcgcctccaa tagcgagctg gtggtgccgc tgttcaagga cggccgcttg
     1261 cttggcgtgc tggatctcga cagcccgtca ttggcacgtt ttagtgcggc ggatcaggcc
     1321 ggtgtcgagc ggttggtcga ggtgtttgtt gcgctgactg actgttaagg ttgcaggtat
     1381 gaaaggagcc cggtcgatcc gggctctttg ctatctgggg ttgcgaagtc aggccttggc
     1441 ggccatcttg gcgagcatct tcagctcgcc cggcaataca gcctgcaccg agtcgcgcgc
     1501 tgtgattcgc gcatagtgcg cactcaggtt aggccagcgc tgcgcgtcca gttgctcgtc
     1561 gccgtgctgc atattgatca gctgacaggc caaggccagg tcagccaggg tcaggctgtc
     1621 ggcaaccagg tattgggccg agccaagggt cttctccagg tagtcaaagt gtgcgggcag
     1681 cttttcgtgc agggcttttt gcacggcggc ttcgtcgcag ccctgaccca tgctgggttt
     1741 cagtgcgcgg ttgcggaaca ctgtgaaggt gcacaggggc gctagttcat aatcggcata
     1801 tttctccagc cagcgtacct gcgcgcgttg ttcggcgttc tggcctagca ggttgctctg
     1861 atctggatgc ttgtcgtcca ggtactggca gatcacgctg gagtcggcaa ggctgaagtc
     1921 gccatccttc atggccggaa tacgccccag cgggctcatg tcgcggtacc aggcaggctg
     1981 gccgaacggc aggatgattt ccagttggta gtccaggttc ttttcagcca gaaacaggcg
     2041 aactttacgg acgaagggcg acagcggggc accgtaaaca gtcaggctca tatgcggtac
     2101 tcataggcag ggcggtagcg cgcacgctgc gcgctggagg ggttagtcgt agatgttctt
     2161 cttcttccac tcgtcatcgc cgccaagaga ctccaggccg gcatccagtt cagtggcttg
     2221 gtcagtggtc ggcttattgc tttccagaac catggcgttg gcgcgtgcca gttgttcttc
     2281 gaatttcttc agcgcgttct ggtaaggagc ggggtcaggt tgtttacgca ggtactgcac
     2341 gccgcgctcg aatgccaggc gtgcctggcc aggctggttt tgctgcaggg cctgttggcc
     2401 gaggttgccg aaaaactcga tgtgcacctg ggtcagcata tggcgcactt ctttcagcca
     2461 atgcttggct tcgttggtcg ggataattcc gtttttggcg caacgggtca gttgaccgtg
     2521 gaggttttcc agttggtagc gtacttcctt ggctttggct tcattgagaa taggttgcgg
     2581 cggattgttt acgggaatcg actcgccttg ggcgatcagc ttgcgcagct cttcaatgcg
     2641 cgtcttgagg gcgttattgc ccttgtctaa aggcagcatg cgttcactga agtgcagctg
     2701 caggcggctc agcaataact tcaaggctgg cgtcatcagt tggccgggga gggcttcggc
     2761 aagatctgtg cagcggcgca ggcgatcatt gaggtcggct ttgatgcgtg ccttttccag
     2821 cttgttgttc tcgaccatat gattgatgta tgcgatggcg atcaatatgg cgatgccaga
     2881 gacaatcaat atggtaatca cgagtgggga catgggtggg gcctcgatca cctgttttcg
     2941 tccgagtgta gtgcgttagc caagaggctg gtagcgctgc ctcacattcg tagctgaagt
     3001 ctttgattta taaaattttt tacacagagg ttgacgagcc ttgcaaggct ccatagaatg
     3061 cgcgccactt cgtagcgcaa tgcctcacgg caaaacgtgt agaagccgga tagaagtttt
     3121 agtttccggg ctgcgtcccc ttcgtctagt ggcctaggac accgcccttt cacggcggta
     3181 acaggggttc gagtccccta ggggacgcca cttcttaatg aagtgtgctg aaaaaaacca
     3241 agttgtatgc gggaatagct cagttggtag agcacgacct tgccaaggtc ggggtcgcga
     3301 gttcgagtct cgtttcccgc tccaaattaa ggtgatctgc ttaggcaggt agccgacaag
     3361 ttccaaggtc cccttcgtct agtggcctag gacaccgccc tttcacggcg gtaacagggg
     3421 ttcgagtccc ctaggggacg ccatttttag taccaagttg tattgcggga atagctcagt
     3481 tggtagagca cgaccttgcc aaggtcgggg tcgcgagttc gagtctcgtt tcccgctcca
     3541 ttttctttca atagaaagca acaagaacgc cgctcactga gcggcgtttt tgtttctgtt
     3601 gtttttgaag catgtaaaaa agcccgccgg ttatgccgtg cgggctttct tgtttctggc
     3661 tgaggattag tgtttgctgc cctgcgcggg catggccagc agctgctttt cctggttcca
     3721 gtcgaagggt tcgtcgtttt cttcggcgta gaagcggcgt tcttcgaggg tctggtacat
     3781 ctcgatttct tcgtcgggca taaagtgcag gcagtcgccg ccgaagaacc acagcaggtc
     3841 gcgggggacc aggtgggcga tttgtgggta gcgctggaag atctggctga tcaggtcctg
     3901 gcccaggtag aggctggctt ccggatcgcc cggcaggtcg ctgagcaatt cgtcgtagcg
     3961 ctccaggtac atggcgtggc tgtcatcgag aatctgctcg gcttcgccca aggcgaccag
     4021 gatggtgcgc aggtgctgca gcagggcgag gtgatggtcg atgtgggtgt tggccatggg
     4081 gcaatcctgt aggtaaaacg ggcgcgcaag tataaggctc gcgtcgcccg ctgtcctgtc
     4141 ggtttaacgg accttgccgg agcccaaggt cagctcttcc ttggcgaagt catcgacatc
     4201 gatcaccacc cggcgcgccg cttcggcctg atggaggctg tgtgcctctt ccgggttgag
     4261 agtcccagca gccagcgcgg catcgatcag gctttgtcct ggcagctcct gaacctggcc
     4321 ggctttgacg gccttgtgca gttttttctg caggggctgt acgtcctgca gcaggttcat
     4381 cgcatattgc agtgccccca ccgggtcctg ttcggcttgt gggcgatagc agccttcgag
     4441 cagttcctcc agcgcagggt cgccgctgtt acggccgatg atgccagcca cttcggcgtc
     4501 cagttcatcg cttgggcctt tgtgacggcg gcccagcggc aataccagca ggcgcagggc
     4561 gcaaccgagc agtttgctgg ggaagttgct gagcagctct tccagcgcct gttcggcctt
     4621 gcccaggctt tcctccacgg cccagcgcag catgggctgc gaatgttccg ggtaaccgag
     4681 gtcgtggtag cgcttgagcg ccgccgaggc caggtacagg tggctgagta catcacccag
     4741 gcgcgccgac agtcgttcgc gacgtttcag ttcgccgccg agcagcatca tgctggtgtc
     4801 ggcgagcagg gcgaaagacg ccgccagccg gttgagcgcg cggaaataag ggcggctgat
     4861 acggtcgccc ggcacctggc tgaaaacgcc aaagccgagg ctgagcagca agctgctggc
     4921 ggcgttgctg acggcaaagc cgatatgttg cagcagcagc tcatcgaact cctgcagcgc
     4981 ctggtcctgg tctgcatgac tggccaaggc catttccttg agcacataag gatggcaacg
     5041 aatcgcgccc tggccaaaga tcatcaggtt gcgcgaaagg atattggccc cctccacggt
     5101 gataaagatc ggcgcaccct gccacgagcg cgccaggtag ttgttcgggc ccatgacgat
     5161 acccttgccg ccgtgcacgt ccatggcgtg gctgatgcat tcgcggccac gttcggtgag
     5221 gtggtacttg aggatggccg agagcaccga gggtttctcg ccaaggtcca ccgcattggc
     5281 agtgaggata cgtgcgctgt ccatcagcca ggcgttgccg ccaatgcgcg ccagagcttc
     5341 ctgaatgcct tcaaaggcgg ctaacggcac gttgaactgt tcgcggacct ggctgtactg
     5401 gccggtggtc aggctggtga acttggcagc gccggtgccg actgcgggta gggagatgga
     5461 acggcccacg gacaggcagt tcatcagcat catccagccc ttgccgagca tctcctggcc
     5521 gccgatcaga tattccagcg ggataaacac atccttgccg gagttcgggc cgttcataaa
     5581 ggccgcgccc agcggcaggt ggcggcggcc gatctcgacg ccgggcgtct cggtaggaac
     5641 cagggccagg ctgatgccga ggtcttcctc ttcgcccagc aggtgctcgg ggtcataggc
     5701 tttgaaagcc aggccgagca gggtggcaac cgggccgagg gtgatatagc gcttttccca
     5761 gttcaggcgc agaccgataa cttcctcgcc ttgccactgg cccttgcaga tgatcccggt
     5821 atccggcatg gcgccagcgt cggagcctgc cagcgggccg gtgagggcaa agcaggggat
     5881 gtcttcacca cgggccaatc gcggcaggta gtggtcgcgt tgggcgtcgg tgccgtagtg
     5941 cagcagcagt tcggccgggc cgagagagtt gggcaccatc acggtggagg ccaggtcgcc
     6001 ggagcgggtg gcaagcttca tcgccacctg ggagtgggca taggcggaaa agcccttgcc
     6061 gccgtattgc ttggggatga tcagggcgaa gaagccatgg ctcttgatat gtgcccaggc
     6121 ctctgcgggt aggtccatct gctggccgat ttgccagtca ctgaccatgg cgcacagctc
     6181 ttcggtcggg ccgtcgataa atgcctgctc ttcctcagtg agttgcgcct tggggtaggc
     6241 cagcagggtg tcccagtctg gccggccact gaacagttcg ccatcccacc acaccgtgcc
     6301 ggcttcaatg gcgtcgcgtt cggtggccga catcggcggc agcacgcgct tgaaccaggc
     6361 aaacagcggt gcagtgagca ggctgcggcg ttgttgcggc aacagcagtg gtaaggccgc
     6421 cgccagcagt aacagccaga gtacgagcat cagccaggcg ggggcatggc tgaacacgcc
     6481 catcagaatc agaaaaccgg ccactatgcc cacagcgggc aggggggcgg tacgccggtg
     6541 agcgagaaag gccgtgccaa gcagcagggc gagtaaccag agagcgagca tgcagaatcc
     6601 tccatgatga caatgcgttt gtacgcacca tcacgagcgt agtcgtcatt gatggcgaca
     6661 agagcggtga gcttggccga atcgtcgtga cattggccgg ccccgcatcg ttagactggt
     6721 ttctcatatc atggagaaac cctgatgcgt gaatacctcg agcccggccg cttcatcgat
     6781 agtgaccatc cagcgctggt tgagttcgca gaaaggtttc gcggggccag ccgcgacccg
     6841 cgtgagcagg ccatccaact gtactatgcc gtacgtgatg aaattcgcta caacccctat
     6901 gtattcagcc gcgacgccga caccttgaaa gccagccacg cattgcaggc gggggaaagt
     6961 tactgcgtgc ccaaagccac cttgctcgct gcctgcgctc gccattgcgg cataccggcg
     7021 cggatcggcc tggccgatgt gcgcaaccac ctggctacgc cgcgcttgct ggagatgctg
     7081 cgcagcgaag tattcgccat gcacggctac accgagttgt acttggacgg ccgttgggtc
     7141 aaggccacgc cggcgttcaa cctggcgctg tgccgggtat tcaaggtggc gccgctggag
     7201 ttcgatggcc tgagcgatag tgtgtttcac cccttcaacc agcagggcga gcgttatatg
     7261 gagtacctgc acgaccacgg ccagtttgcc gatgtgccgg agcagctgtt cttcggtcat
     7321 ctggccgctt gctacccaca tctgtttgcc gaggatgcgc tgtcgatcag tggcgacttc
     7381 gccgccgagg ccgtgcccgg ttgatgtatc gacagggctg ataagcacca tcggaaactg
     7441 ccgggtttaa tctgagccta gcgctggtag gctgctgccc tgctcggggc tggtagattt
     7501 cccctaaaag ggctgcgatg ccggtgtacc aggcgctaga cttgggcgca ctttgcgctg
     7561 catgacgcag cccctctcga ctttcttttt tatctgatca ggtgtgcgca tttttatgag
     7621 tactgctctg tccattcgcc agttgaccaa gacctatggc aacggcttcc aggcgctgca
     7681 tggcatcgac ctggatgtgg ctgaaggtga tttcttcgcc ttgctcggcc ccaacggtgc
     7741 cggcaaatcc accaccatcg gcatcctctc gaccctggtg accaaaagta gcggcaccgt
     7801 gaatgtgttc ggtcacgacc tggacaaaca gccctcggcg cttaagcgct gcctgggcgt
     7861 ggtgccccag gagttcaact tcaaccagtt cgagaaggcc ttcgacatcg tcgtgaccca
     7921 ggccggttat tacggcatcc cggcgaaaat cgccaaggag cgggccgagc agtacctcaa
     7981 ccagctcggc ctgtggggca agcacaatgt gccgtcgcgt gaactgtccg gcggcatgaa
     8041 gcgccgtctg atgatcgccc gcgccctggt gcatcagccg cgtttgttga tccttgatga
     8101 gccgaccgct ggggtcgata ttgagctgcg ccgttcgatg tggagctttc tcaccgagct
     8161 gaaccagcag ggcatcacca tcatcctgac cactcattat ctggaagaag ccgaacagct
     8221 gtgccgcaat atcggcatca tcgaccacgg gcggattgtc gagaacacca gcatgaagga
     8281 cctgctgaaa aaactgcatg tggagacctt cctgctcgac ctcaaggagt cgctgctggt
     8341 tgcgccgcag ttgattggct acccggcgca gttactcgac gggcataccc tggaagtgca
     8401 ggtggacaag gctcagggca ttaccgagtt gttccgccag ttggctttac agaatgtcga
     8461 ggtgctcagc ttgcgcaata aaaccaaccg cctggaggag ctgttcgtct ccctggtcga
     8521 gaagaatctg gcgaaggtgg cggtatgagc acggcttatc tgaactccga attcaccgcc
     8581 aacatggtgg cgctacgcac catcgtttac cgcgagatcc gccgctttac ccgcatttgg
     8641 ccgcagaccc tgctgccgcc ggcgatcacc atggttctgt acttcgtgat cttcggcaac
     8701 ctgatcggta gccagatcgg cgacatgggt ggcttcactt atatggagta catcgtgccg
     8761 gggctgatca tgatgtcggt gatcaccaac tcctacggca acgtggtgtc gagcttcttc
     8821 ggcagcaagt tccagcgctc catcgaggag ctgatggtct cgccggtgtc gccgcatatc
     8881 atcctcaccg gctatgtggt cggcggcgtg ctgcgcggcc tggcggtggg ttttatcgtc
     8941 accttgctgt cgctgttctt caccaaactg caggtgcatc acctcggcgt gacggtgctg
     9001 gtggtgctgc tgaccgcgac catcttctcc ctgggcggct ttatcaacgc cgtgttcgca
     9061 cgtaacttcg atgatatttc gatcattccg acctttgtgc tgacgccgct gacctacctg
     9121 ggtggggtgt tctattcgat caccttgctg ccgcagttct ggcagaccgt ctccctggcc
     9181 aatccggtac tccatatggt caacgccttc cgttatggca ttctcggggt gtccgatatc
     9241 cgcatcggcg tggccatcgg ttttatgctg gtcgccaccg ccgtgctgta tggcgtgtgt
     9301 atccgtttgc tggtcagcgg ccggggtatg cgccagtagc cgacagccaa cattgggaaa
     9361 catttttaac ggctgatctg cgcggctcgg ccgttagact cagcctctac aagacattag
     9421 gggttgtata tgtttgcccg cgcgcctgct cttctcggaa cagtcttttt gctgtgttcg
     9481 cttgtcagtg tgcaagcgca cgccaatgaa gggccgcttg tcgaggtggt caaggctgag
     9541 cgaagcctgg tgcgtgacga gctggtgacc ttcggttcgc tacgccctaa cgagtcggtg
     9601 atgatccgcc cggaaatcgc cgggcgagtg gcgcagctgc attttcgtga aggtcagcgc
     9661 gtcgaagcgg gtgcgctgct ggtcagcctg gatgactcga ttacccgtgc cgagtttgcc
     9721 caggcgcggg ccaacctgaa cctggcggaa aagaacttcg agcgcgcgca gatgctgttc
     9781 aagcgcggcg ccagtaatgc ccaggcgctg gatgaagcca gcgcccagca acaggcctca
     9841 cgcgccagcc tggcgctgtc gcaggcccga ttggacaaga cccgcatcct cgccccctac
     9901 gacggtgtgc tgggcctacg cgcagtcagc cctggtgatt acctcagcga aggccaggac
     9961 atcgtcaacc tggaagtgct cgatccgctc aaggtggatt tccgcattcc gcaaaaagct
    10021 gtcagccaga ttcgtctcaa gcaggtgatc gaaatcagcc tggatgctta tccggatgag
    10081 cgttttactg gcgagatttt cgccatcaac ccgcgtctgg acgaggccgg gcgcagccag
    10141 gcgattcgcg cgcatatcag caacagcgat ggccgcctga gccctgggca gttcgtgcgg
    10201 gtttcggtga ttctcgccga gcggccgaat gccctggtga ttccggaaga agccattatg
    10261 ccggtgggcg agaaactgct ggtcaacctg gtggtggatg gcaaggtcga gttgcgcgag
    10321 gtcaaactgg gcaagcgcct ggctggcaag gttgaagtgc tggaaggcct gcagggtgat
    10381 gaaaccctga tcagcgccgg ttggcagaag gttcgggccg gcgcgcctgt gcgcaccaag
    10441 gtggtggagc ccgcgccatg accctgtcgg atatctgcat tcgccggccg gtatttgcca
    10501 ccgttctgtc gttgattgtg gtgctgctcg gtttgatggc ctatgaccgc ctggccgttc
    10561 gcgagtaccc aaatatcgac gtgccgattg tcacggttaa cgtcacttac ccgggggcca
    10621 gcccggagat tatggaatcc caggtcgccc agccgatcga ggatgtgctg tcgggtatcg
    10681 atgggctgga tttcgtcagc acatcagccg ttcggaaaac acccagatca ccacgcagtt
    10741 ccgcctgggc agtaactccg atgaagccgc caatgatgtt cgcgaccgtc tgggtcgggt
    10801 gcgcggcctg ctgccggatg aaatcgcgga gcccattgtg cagaaggtcg aggccgacgc
    10861 ccagccggtg atctggctgg cgttctacag cgaacgctac agcgccatgg agatcaccga
    10921 tgtgctggag cgggtgatca aggatcgcct gcagaccatt cccggcgtct ccgaagtgca
    10981 gattcgcggc gcgcgcacct tcgccatgcg catctggtta gatccggaga agctcgccgc
    11041 gcacaacctc accgtgcagg acgtggaaga cgcgctgcgc cggcagaacg tcgagattcc
    11101 cgccgggcgt atcgaatcgc agcagcgcga attcaccgtg ctctcggaaa ctgacatgaa
    11161 caccccggag cagtttaatc agctgatcct cgacgactcg cgcggttacc tgctgcgcct
    11221 ggcggatgtc ggctacgccg aaatcggcgc ccaggatgaa cgcagcatcg tccgttacaa
    11281 gggccagccg gccgtgtcga tgggcatgat caaacaggcc accgccaacc cgctggacat
    11341 ctccgatggc ctagcggcag ctttgccgga tgtgcgcgaa ttgctgccgg aaggcatgga
    11401 aatggcggtg gctaacgaca actcgctgtt tattcgcgag tcgatcgaca atgtgtttat
    11461 taccatctgg gaggcggtgg ggctggtcat cttgatcatc tttatcttcc tgcgctcgct
    11521 gcgcgccacg attattccgc tggtgaccat tccggtgtcg ctgatcggcg cgtttgccct
    11581 gatgagcctg atgggcttca ccatcaatac cctgaccctg ctggcgatgg tgctggccat
    11641 cggcctggtg gtggacgatg ccatcgtgat gctggagaac atccaccggc atatcgagga
    11701 gggcatgcag ccgctgcagg cggcgctcaa gggcagccgc gagattgcct ttgcggtgat
    11761 cgccatgacc atgaccctgg ccgccgtatt cgcccccatc ggctttatgc agggcaccac
    11821 gggcaagctg tttaccgagt tcgcctggac cctggccggc gcggtgctgg tgtccggctt
    11881 tgtcgccctg accctgaccc cgatgatgtg cgcgctgttg ctcaagccgc atcaggccgg
    11941 cgagcggcat aaccgcctgt acaacctgat cgaagatttt ctcaatggcc tgacctacag
    12001 ctacaagcac ctgctggcca gggccctcag cgcctgggtg ctggtggtgg gcgtgctgtt
    12061 actcaccgtg gtggcgtgtg tgctgctgtt ctcggggctt aaatccgagc tggcgccgac
    12121 cgaagacacc ggcaccatta tcggtgtgtt caacggcccg gacggcgcca ccatcaatta
    12181 caccgggcgt tatgcccgcg agatcgaagc cgcttacgcc agcatccctg agaccaaccg
    12241 ctatatggtg attgccggct ttccgactgt ggcccagggc atctccttta tgaagctgga
    12301 agactgggcc gaccgttcgc gcagtcagtt cgagattcgt aacgagttgc tgcccaaact
    12361 gcaggatatc gccggggtgc gcgccttccc ggtcaaccgg ccgccgctgg ggcagagcgc
    12421 gcgtaaccag ccggtgaact tcgtgattcg ctcttccatg gagtacagcg agctgcaggg
    12481 ttatgtcgat gaattgctca actcgctacg cgattatccg gggctggaaa gcctggacag
    12541 cgacctcaag ctcaatgcgc cgcagctgaa agtcgcggtc aaccgtgaac aggcggtggc
    12601 ggtgggcacc gatgtggcga ccattggccg cagcctggaa agcctgttcg gcagccgcca
    12661 ggtgacgcgc ttcaagcaga acggcgagca gtacgacgtg ttggtgcagt tgcagggcgt
    12721 ggaccgcagc aacccgcagg acctggaccg cgtctatgtg cgcggccgcg atgagagcat
    12781 ggtccagctg tccaacctga tcgatgtgcg cgagaccgta gcgccgcgcg agctcaacca
    12841 cttcaaccag ctgcgtgcgg taacggtaag cgccaacgtt ggctccggct acaccctggg
    12901 cgaagccctg gcgcatctgg agactacggc gcgcacggtg ttccctgcgg agactcagta
    12961 cgactacacc ggcacctcgc gggacttcaa ggagtccagc gccggcattg cgctgatctt
    13021 cgccctggcc ctggcgttta tcttcctggt gctggcggcg cagttcgaaa gctttaccga
    13081 cccgctgatc atcctgttca gcgtaccgct gtcgatggcc ggcgccttgc tggcgctgac
    13141 attattcggc ggtaccctga atatctactc gcagatcggc ctggtgacgt tgatcggcct
    13201 gattaccaag cacggcatcc tgatcgtcga attcgccaac gtgctgttgc gtcagggcca
    13261 tgagctgcgt ggtgcggtga tcgaggcgtc ggtgcagcgc ctgcgaccga tcctgatgac
    13321 caccggggcg atggtgctgg ggtcgctgcc gctggcaatt gccactgggg ccggtgcgga
    13381 gagccggcag caaatcggta tggtgattgt tggcggcctg ttggtcggca ccttcttcac
    13441 cctgtttgtg atcccgaccc tgtatatgct gctgcgccgc tggcggccga taaagcagga
    13501 aatgcctgag gcagtggccg cctgacaggt gtaggagcgg gccatgcccg cgaaatagcc
    13561 ggatcgcggg catgacccgc tcctacgaga tcgtgttagt cgatctttag caccagcttg
    13621 ccgaagttct cgccgctgaa cagtttcagt agggtttccg ggaaggtctg cagtccgctg
    13681 acgatatctt ccttggattt cagcttgccg ctggccatcc aaccgcccat ctccgcagcc
    13741 gcttcggcga agcgtggggc gtaggtcatc accaccatgc cttccatgcg tgcgctgttg
    13801 accagcagat tgaggtagtt ggccgggcct ttgacggcct gcttgttgtt gtactggctg
    13861 atcgcaccgc agatgatgac ccgcgccttg aaggccaggc gggtcagtac ggcatcaaga
    13921 atgtcgccac cgacattgtc gaaatacacg ttcacgcctt tcggacactc gcgcttgagg
    13981 ccggcgatca cgtcttcgct cttgtagtcg atggcgccat cgaagcccag ctcatcaatc
    14041 aggaacttgc acttgtccgc accgccagcg atgccgacca cgcggcaacc tttgagcttg
    14101 gcaatctgcc cggcaatgct gcccactgcg ccggccgcac cggaaatcac tacggtttcg
    14161 ccagcttgcg gtgcgccgac atcgagcagg gcgaagtagg cggtcatgcc ggtcatgccc
    14221 agggccgaca agtacagcgg cagcggcgcg cgcttgggat cgactttata gaagcccttg
    14281 ggctcaccga ggaagtaatc ctgtacgccg agtgcgccgt ttacatggtt gcccacggca
    14341 aagtccgggt gctgcgaggc caccacttca cccacgccaa gggcgcgcat cacttcgcca
    14401 atgccgaccg gcgggatata ggacttggca tcgttcatcc agccgcgcat ggctgggtcc
    14461 agcgacaggt aggcgttctt caccagaatc tgcccggggc cgggttcacc gacgctgttt
    14521 tccacatagc tgaaggtctc acggctgggc aggccgaccg ggcgttgggc aagcaggaac
    14581 tgacgattga gtggggcatt catggcaggt actcattaag cggaaagact gttgatagcc
    14641 cgtagcggcg tgatgggcaa gatttgcccg cgggacgaat ggtggttaat ccattcgaat
    14701 gataggtggg ctggggtgtt aatcactcgt ttggatgaag ccatcaccag cagtttgata
    14761 gtgctgcctt gggactgggg agctgattag actcagcgca gtttctaatc cttgcgagga
    14821 cacgcagatg agctttagct tttccggccg ggttgccctg gtcaccggtg gtgctgctgg
    14881 tattggtcgt gccacggcac tggcattcgc caacgaaggc ctgcaggtgg tggtgtctga
    14941 cgttgatgtg gcgggcggtg agacaactgt cgctctgatc cgtgaagcag gcggcgacgc
    15001 gctgttcgtg cgctgcgacg taacccgcga cgccgaggtc aaggtgctga tggagcgcac
    15061 cctggctgct tacggccgcc tggattacgc cttcaataac gccggcatcg agatcgagaa
    15121 gggcaagctg gccgaaggca gcgaaagcga gttcgacgcg attatgggtg tcaacgtcaa
    15181 aggcgtttgg ctgtgcatga agcaccagat cccgttgctg ctggcccagg gtggcggcgc
    15241 catcgtcaat accgcctcga ttgccggcct tggcgccgcg ccgaagatga gcatctatgc
    15301 cgcctccaag catgcggtga tcggcctgac caaatcggcg gccgttgaat acgcgaagaa
    15361 gaaagtgcgg gtcaacgcgg tatgcccggc ggtgatcgac accgacatgt tccgccgcgc
    15421 ctatgaatcc gacccgaaga agggcgagtt cgccgctgcc atgcacccgg tcgggcgtat
    15481 cggcaaggtc gaggaaatcg ctaccgcagt gctgtaccta tgctgcgata acgcggcctt
    15541 caccaccggc caggcgctgg cggtggatgg tggcgccacg gccatctgat ttccttaagt
    15601 aataaaaaag cgctcatctg ggcgcttttt ttatacctcg ccatttcttt cacacgattg
    15661 gccactaaag tcactgcagt gttctacgcc tctgtacaag atcgcccagc gctttgatca
    15721 aggagtaagc aagccgtgac agctgacccg ttattgaccc tgttcctgcc catcgccctg
    15781 ggcatcatca tgcttggcct gggcttatcg ctgaccctgg cggattttgc ccgggtggtg
    15841 aaatttccca aaccggtgct gatcggcttg agttgccagc tgttgctgct gcccgtggtg
    15901 tgttttttcc tggccaaggc ctttggtctg gccccggcgc tggcggtggg gttgatgctt
    15961 ctggccgcct cgccgggcgg caccacggcc aatctataca gccacctggc ccacggtgac
    16021 gtggcgctga atatcaccct gactgcggtc aattcggtga ttgcggtgct gaccatgccg
    16081 ctgatcgtca acctgtcgct ggcttacttt atggaaggcg atcaggccct gccgttgcag
    16141 ttcaccaagg tggtgcaggt atttgtcatc gtgctcggcc cggtggccat cggcatctgg
    16201 atacgcagcc gtttccccgg gtttgccgag cggatggaaa aaccggtgaa gatcatctcc
    16261 gcgctgtttc tgttgatcat catcctgctg gcggtggtca aggattggca gacctttgtc
    16321 gattacgcgc cgctggtggg cggcgcggcg ctggcgttca atttgatcag cctggccgtg
    16381 ggctactggg tgccgcgctt gtgcaaactg agcctgcgcc agtcgattgc catcggcatg
    16441 gaaatcggca tccacaacgg taccctggcg attgccctgg cgctgagtcc gatgctgctg
    16501 aataacgcca ccatgtccat ccctgcggcg atctacagca tcatcatgtt ctttaccgcc
    16561 gcggccttcg gctggtgggt gaacttcgcc catggcaaag agctgaaaac cactgagtag
    16621 tggattgtcg accataaaaa agcccgtcct agtgacgggc tttttattca gctgccggtt
    16681 tagtgcagcg gcagttcaac accctcgaac agctcttcca actcaacctt gctgtggcat
    16741 tgcacggctt tggccatcat gtcgcgggtc aggtgcgggg caaacttctc gataaagtcg
    16801 cacataaaac cgcgcaggaa ggtgccacga cggaagccga tcttggtcac gctggcttcg
    16861 aacagttcgc cggcatccag caccaccagg tctggatcga gcttggggtc tacggccatc
    16921 ttggccacga tacccacgcc cagacccagg cgtacatagg tcttgattac gtcggcgtca
    16981 gcggcggtaa acaccacttt cggcgtcagg ccgcgatggc tgaaagcttc gtcgagtttg
    17041 gagcggccgg taaaaccgaa cacgtaggtg acgatggagt attcggccag ggcctccagg
    17101 gtcagctttg gcagtttggt cagtggatgg ccctggggca ccaccacgca gcggttccag
    17161 cggtagcagg gcatcatgat cagatcgttg aacagttcca gaccttcggt ggcgatggcg
    17221 aaatccacgg tgccatcagc agccatttcg gcgatctgca tcggtgtgcc ctggtgcata
    17281 tgcagggcca cgtcggggta ttgcttgata aaggcactga tcaccggcgg cagggcatag
    17341 cgcgcctggg tgtgagtagt ggcgatggac agggtgcctt tcttctcgtt ggagaattcc
    17401 tgggcgatct gcttgatgct ttcgaccttg cgcaggattt ctccggcggt gttgatgatg
    17461 cgttcgccgg ccggggtgat gcgggtcagg tgcttgccgc tgcgggcgaa tacttcaacc
    17521 cccagttcgt cttcgagtag gcggatctgt ttgctgatgc cgggctgcga ggtgtagagg
    17581 ctttgtgcgg tggcagaaac gttgaggtcg tgatgcgcga cttcccagat gtagcgcagt
    17641 tgctgaagct tcataaaaat ccctcaaagc aaaaagacag ccgttcaggt taccggcgtt
    17701 ttatataacc ataatgatga ttgaagcatt aaatctagac caaaaactaa aaagtgctgc
    17761 gcttgtttaa agatcaccgc gactgcggtg ttgcagcatt ggcaccaggt acaccggcac
    17821 ttcggacagc tgtaccagcc gcgacgcggt gcgtcccagt ggaatgtcca tatccgtgcc
    17881 gtggctgtgg ctacctagga ccagcaaatc cacgccgagt tgctgcgctt catcgagtat
    17941 caccagtggt gggtcgccct ggatcacgcg caccgccttg atcagctcca gatcatgcag
    18001 ggcttcgccg atctcatcgc gaaagccctc cagcacgcgt tgctcgatac tcgccatcac
    18061 cgtgctcagg ccattggtgc gcaaatcctt gaggcgttcc tcatctaaat aggtctgcag
    18121 caccgactcg gcaaacagac ccatgggttc caccgcgtgc accacataca ggctggcatt
    18181 gaaggagcgg gtcagcgcca gggcgtgttg caggatgtag ggggcgtata ggccaagatc
    18241 agtggcatag agaatcgagc gaatcatgcg gcctccttga ctgcctgact ggcgggcctg
    18301 atctgagctt agcagcgcca tcgataagtc aaagaaagct gaggttctga ggacgaatgg
    18361 ttatatacga ggggcgggag ttgagcggcg agggcggttg aacgccccga gcgggggtta
    18421 gagttgcggc tcgttgctga tgccatgagg gacgtgaccc gtggcgacca cttcgcgggc
    18481 tttttcacag tgcccggctt ggtcgtcgaa gaacacgtcg gcgccgaagg tttccagaat
    18541 cgcggctttt tccaggccgc cgaggaagta cgactcatcc aggcggatat tccactcgcg
    18601 cagcgtacga atcacccgct catgggccgg cgccgagcgt gcggtaacca gtgccgtgcg
    18661 aatcgggcat tgctcttcgg gaaactcctg ctgcaggcgg tgcagggcgg cgaggaacgg
    18721 cttgaacggg ccgccgccga gcagctcacg ggccgactca cgttcgtgac tctggaaggc
    18781 ttccaggcca gcctgctgat agacccgctc ggattcatcg gaaaacagca cggcatcacc
    18841 gtcgaaggcg atgcgcagct cattgctggc cgcccgccga gcgccaccgg agagaatggt
    18901 cgcggcaccg aagccggcac gcagcgcgct gcgcacatcc tcggcgtggg ttgagagaaa
    18961 caggtggcag ccgaatgcgg ccagataagg gtctgggctg cgcccgccga caaaggccgc
    19021 gcgggagatg cccaggccgt agtgctggat cgagttgaac gcgcgcaggc cggtgtcggc
    19081 gctattacgc gagaccagaa tcacctcgac ccgctgctgt tcgagggccg tgttcagcgc
    19141 cagcagcttc tcgaccaggg gaaaggcatc gccggggctg agtatctcgt cctcatgctc
    19201 aatctggtat tggcgatagg ccgcgacgcc ctggctttca tagatgcggt ggctttcgct
    19261 gaggtcgaac agcgcccgcg aggagatcgc cagtaccagt ttgtcgccta atcccttgct
    19321 catggctgtg tcctattgcg tgcgttgttc gaggaaacgc agggtctggt aaagcatctc
    19381 ggtacgcggc atggcgcagc cggcctgggc tgcagcggcc agcggcgcgg cgtagatggc
    19441 atgcagttcc agtgggcgct gcagggcaaa gtcgtggtac atgctcggca ggtaatccgg
    19501 catgcgatcg gtggccgcca gcagcttgcc ggcaaagcct gcgggcaact gatagccgca
    19561 ggcgctggcg gcatcgacca cttcctgcat gatcgcttgg atcagcgtac ggctgtcgcc
    19621 attgcccatc agcgccgtgg tggcgctgtt cagcagcacg gccaggccgt tgtagggcac
    19681 gttccacacc agtttctgcc agcgtgcctg gttcagctcg gccatggcgg tggagtccag
    19741 ccccgcagcc tgaaatagcg cgctgccttg ttcgaggatc tgcagacggc tatcggcatc
    19801 gctcgccgga cctgagtgat agcccaggtt gacgctgccc agcgcctggt gctcgaccac
    19861 gcccggtgcg ctgcggtggg cgcagatata gcaaaggccg cccagcaggt gcagcgagtc
    19921 cggcagcagc gggcgcaact cgtcttctac ggcaaggccg ttttgcatca gcaccacctt
    19981 ggcgccgggc gcggcggcct ggctgatcag cggcgcaagt tcggcgttgc tggtggtctt
    20041 ggcgccgatc agtaaccaat cgcatggcgg catatcggct gcggactgat aagcctgcac
    20101 gttatccagg tgcaggttgc cgtgtacggc gctgttgacc tgcaagccct ggctggcgac
    20161 tgcggcaaat tcgctgcgca gtagaaagtg cacgtcaaag ccggcgcggg ctagcatcag
    20221 gccataaaag ccgccaattg caccggagcc gataatgcca atacgtggag atgggttcat
    20281 cagggtaatt cctctgcggg gcgcgacagg gcagtgtcca gcgcctgggt gagttcggcg
    20341 agctgcaggc gactttgcaa ggcgccataa aattggccat cttgtactac gaataacgcg
    20401 ggtagatgaa atacctcata acgcgtcact aaaccgccac tgttggccgc gtcgacccag
    20461 gccaggcgct gtatcggcag gggcaactta ggcagctcac ggcgcgccca acggcaactg
    20521 gcacaaccta tgctggtaaa tatcagcagt gaagttccag gcaagtcgag tagatggcga
    20581 tctgcatcta aatcggtcag ttccagttca gtcactatac tggccaatgt ggtatcaaat
    20641 tgatggcatc cgaggtagtc atagatgcgt cactttctgc gtcatcccag tgatatgccg
    20701 gttgaattag tgctgcgcaa acaggcattt gtacccaaag agcggctcaa caatatcagc
    20761 ctcggcggtg tggcttgcaa ctccaaccgc gcttttcgcc gtggtacggc catcgaactg
    20821 cgtattccgc tgttcggcga gcatgcgcgc tatccgggcc tggtggcctg gtgccgcaag
    20881 caggagggta aatacctagt aggtatcgcc tttgtcgatg aggacacgct gtttcgcgcg
    20941 cgcatggtcg agcaggtctg ccagatcgaa cattatcgcc atcaacgcga gcaggaactg
    21001 ggctgcagcc tgccggttga ggtggtggcg caggaatgga tcagccggca tgccgccgag
    21061 ttctcccgcg ccagcctgaa ttagtgattg ccccgcctgc aggtcgttga aaaacgtcgg
    21121 cgaggcaggc aagacaaggc gctacgtagt aacagccttt ggctggtcaa agcgccgagg
    21181 aagcggagtt tactgttgta aatgagcatt ccgaggccgt ttttaacgca gtattgccaa
    21241 cgcaggtagt ttttcggcga cctgccaagc ccattgtcga gccaggccta tacgcgctaa
    21301 ggttcggctt ccccgctgtg ctcatattgc tgtgctccgc cacacgggga tcgctggcgg
    21361 ccggcacccg tgacctgatg actctgactg atgaatagca cgatggc
//
LOCUS       DASRUM010000073        20103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_203679, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000073 DASRUM010000000
VERSION     DASRUM010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 20103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20103
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203679"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1985)
                     /locus_tag="VFF22_05160"
     CDS             complement(<1..1985)
                     /locus_tag="VFF22_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013693006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZX16236.1"
                     /translation="MNEISVQLHSWLRMQRESLPLAVRADALLRALHACPEVRQAYFL
                     RWQASTAIYSHEGSAPHLPPGFGDPLQASDLALCERLEDEPRLDLGALRQMPCWLAGR
                     VRRAGLNHGQALALALDDGHCDLLLIELQEQASSDWLGLVHQLLQALLASLPQQARGS
                     LLLGSDPQPSLLLDIQARPLQLNAAMHELLGPRELAAVAELLPVNTPQLVRACLQQGR
                     AIEAVEAQYAGQVLIWIFMPDPAEQQVLARCRPATAQVQAEREAAKASRLYRLITENT
                     TDLISRHTPDGRFLDASPASWTLLGYWPETLRGTHVQALFHEQDQQQLVDSTREALEQ
                     DGYHTMTYRIRHRDGHYLWFETASRAIRETYTGAVVEVVSVSRDITARVLAEENKRRL
                     AEVVEANTDLVLFIDPSGHLTHLNPSARHALHIEDGQPLPALDQLLAQDDLLRLQRSG
                     WSTADSQGVWSAEVRLLPLGPHAPLLVSLVLLAHRGRDGEHYYSLVARDMTERELREA
                     QQRRHQDELAHTARLVTLGELASGIAHEINQPLAAVVNYASASQRYLKALGTNPQAAD
                     KVAQGLERINEHANHASEVIKRLRAFLRKGQRRTQALDIASVAREAVRLCQWEASNWQ
                     VTIAEQLADNLPPVYADRILLEQVLLNLLRNAIDANR"
     gene            2221..3843
                     /locus_tag="VFF22_05165"
     CDS             2221..3843
                     /locus_tag="VFF22_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HZX16237.1"
                     /translation="MSIYEQGLAPTAVNHIALSPLSFIERTASVYPHYPAVVHGSIRR
                     NWSETYARCRRLASALAGRGIGKGDTVAVMLPNIPQMLEAHFGVPMIGAVLNPLNVRL
                     DAEAIAFMLQHGEAKVLITDREFHEVIHAAVGMLDHPPLVIDVDDPEYGEGQAVSDLD
                     YEAFLAEGDPQFAWQWPADEWQAISLNYTSGTTGNPKGVVYHHRGAYLNSLGNQMTWA
                     MGNHPVYLWTLPMFHCNGWCYPWTITALAGVHVFLRRVDPAKILTLIRAEQVTHLCGA
                     PIVLNALVNMPAEAKAAIDHPVKAMVAGAAPPAKVIGAVEEMGIHVTHVYGLTEVYGP
                     VTLCAWHAEWDALPLDERAVIKARQGVRYPTLEGVMVADPKTLEPTPRDGETIGEIFM
                     RGNTVMKGYLKNPSATAEAFAGDWFHTGDLAVCHPDGYVEIRDRLKDIIISGGENIST
                     IEVEGVLYRHPAVLEAAVVARPDEKWGETPCAFITLKADQQQVAEADIISFCRDHLAG
                     FKVPKTVVFTQLPKTSTGKIQKFVLRDMAKAL"
     gene            3973..5343
                     /locus_tag="VFF22_05170"
     CDS             3973..5343
                     /locus_tag="VFF22_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/uracil/vitamin C permease"
                     /protein_id="HZX16238.1"
                     /translation="MQIFKRRDGDEQPHWPVGPFKVRLPFVHYRWETAEMLQALIMFV
                     VSLAMIPLLEKYLGLPYDVALAYVVVCGVGFMLPALLGVPMVPGWITPGIPVVLLFLG
                     NFKPGPEAIQALFALQFLVFLIFLILGVTRLGSTLVRLIPNSMKGGIIIGAGIAALMG
                     EISTGGRLANTPISLVIGSLICLYLMFSVSFKGLTERLPLARKIVNYGMVPGMLIAIF
                     IGIAVGEYKMPDVKFGITAPAFGEMWNYLPFSVGFPGLDVFLLAVPTAVIAYIIAFGD
                     IIVGQSLMQRADELRTDEVIENNIDRVHLVTALRNALHAFFAPYPGLAGPLWTAVAAT
                     MAERYKYGRKAMDSIYSGAGTFWITGFIALFALPLVSFFQPVLPIALSLTLILTGYIC
                     LMVGFEQLSNNTERGIAGTMGVVLAVYGAGWGLAAGAALYILVERTHLLKFTTKTSVK
                     GQPEAD"
     gene            5418..7190
                     /locus_tag="VFF22_05175"
                     /pseudo
     CDS             5418..7190
                     /locus_tag="VFF22_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252888.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
     gene            7268..8446
                     /locus_tag="VFF22_05180"
     CDS             7268..8446
                     /locus_tag="VFF22_05180"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017902125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZX16239.1"
                     /translation="MQEVVIVAATRTAIGAFGGSLSSISAVDLGAAVIRRLLTDTGLP
                     AEQVDEVILGQVLTAGAGQNPARQSVIRAGLPHTVPALTLNKVCGSGLKAVIMAAQAI
                     RCGDAEVIIAGGQENMSLSPHVLRGSRTGLRMGHGQLLDSMIHDGLWDAFNDYHMGIT
                     AENLTEQYGISRAEQDAFAARSQQRACAAIAAGRFAGEITPIEIPQRKGDNLIVAVDE
                     QPRASSTLEALGKLRPAFKSDGSVTAGNSSTLNDGAAAVLLMSASKATELNLPVLARI
                     KAHASAGVDPATMGIGPVFATCKALEKAGWSLEQLDLIEANEAFAAQAIAVGRELHWD
                     SAKVNVNGGAIALGHPIGASGCRILVSLIHEMIRRDAHKGLATLCIGGGQGVALAIER
                     "
     gene            8559..9425
                     /locus_tag="VFF22_05185"
     CDS             8559..9425
                     /locus_tag="VFF22_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZX16240.1"
                     /translation="MRTLTTLTGNSQKLDGGAMFGNAPKALWQRWMAPDELNRIDLGC
                     RALLVQEDERNILIETGIGAFFSPELKQRFGVQEERHVLLDSLVAVGLSDADIDIVLL
                     THLHFDHAGGLLAAWEADKPARLLFPNAQFITGHRQWQRACQPHARDRASYIPELLEL
                     LEASGRLQLIDETSSCELLGADWRLHWSDGHTPGQLLPEVAMPDGPVVFPGDLIPGAP
                     WVHLPITMGYDRFPEGLIEEKQALLSDLQARGGRLVFTHDPAVAMGRVVCDDKGRYGL
                     EETQANVRELAE"
     gene            complement(9512..9787)
                     /locus_tag="VFF22_05190"
     CDS             complement(9512..9787)
                     /locus_tag="VFF22_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011266527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HZX16241.1"
                     /translation="MACLLLVALLPGCATVATLNAAKPGAPVVYAGTRLDLYALQGGC
                     CAVERFGCEAPKYAALDLPASALLDTLLLPLSLATAMGLRLSVSGGL"
     gene            complement(9837..10469)
                     /gene="ubiX"
                     /locus_tag="VFF22_05195"
     CDS             complement(9837..10469)
                     /gene="ubiX"
                     /locus_tag="VFF22_05195"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HZX16242.1"
                     /translation="MSGPERITLAMTGASGAQYGLRLLDCLVQEEREVHFLISKAAQL
                     VMATETDVSLPNKPQAMQAFLSEYTGAAPGQIRVYGKEDWMAPAASGSGAPTAMVVVP
                     CSSGSLSAIANGACNNLIERAADVALKERRQLILVPREAPFSSIHLENMLKLSNMGAI
                     ILPAAPGFYHQPQTIDDLIDFVVARILNCLNIPQDMLPRWGEHHQVSGDE"
     gene            complement(10632..11981)
                     /gene="mpl"
                     /locus_tag="VFF22_05200"
     CDS             complement(10632..11981)
                     /gene="mpl"
                     /locus_tag="VFF22_05200"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014819035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HZX16243.1"
                     /translation="MHIHILGICGTFMGSLAVLAKELGHRVTGSDANVYPPMSTQLEA
                     QGIELTQGYDAAQLDPAPDLVVVGNALSRGNPAVEYVLNKGLPYVSGPQWLADHVLQG
                     RWVMAVAGTHGKTSSSSMLAWVLEHAGMSPGFLIGGVPQNFGISARLGGTPFFVVEAD
                     EYDSAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAIERQFHHLVRTIPGDGLII
                     HPTTEPALKRVIEMGCWTPVQTTGDGGQWQARLLSADGSRFEVSFDGKTAGTVDWQLT
                     GQHNVANALAVLAAARHVGVVPELGIAALCGFINAKRRMEKVAEVNGVTIFDDFAHHP
                     TAIATTLDGLRKRIGDAKLIAIVEPRSNSMKLGAHRDGLSESVVQADSVYWYAPPNLG
                     WDLAGTVATSTVPTQVCDSLEAIIAGVKAEAAPGTQVVVMSNGGFGGLHGKLAAALL"
     gene            complement(12124..14028)
                     /locus_tag="VFF22_05205"
     CDS             complement(12124..14028)
                     /locus_tag="VFF22_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007968141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HZX16244.1"
                     /translation="MHNPASRHAQQVLTVAQGRGQLAGPAADSSVARSWLRCLQQHHL
                     DPAQSMPPAVIEHAHMLERREQLQQVLEISSNEMNSLHRQLAGSGHAVLLTDARGVIL
                     NCVTEQAEKRTFEQAGLWLGADWSEACEGTNGIGTCLVERQPLTIHQNEHFRSRHTGL
                     TCSASPVFDPHGELLAVLDVSSARHDVSRQSQFHTMALVNLSAKAIEGCHFLRSYEGE
                     WLLRFHAQAEYIGQFSEGLLAFDGDGRVSAVNQSAINLLGLSRRQLLGRTLIEVFDCR
                     LDDLLGRASAQPSASWPLYTQRGQLLFAMLRGQPRRSAQALAAGSLATPGLCLADAGL
                     QSDFRRALRVYERDVPLLIGGETGTGKEAFAKALHQVSSRGGKPFVALNCAAIPESLI
                     ESELFGYRGGSFTGARKEGMRGKLQQADGGTLFLDEIGDMPLALQTRLLRVLEERRVE
                     PIGGEAQAIDVRVISASHRDLNERVANGEFRQDLFYRLNGLVIELPPLRERSDKLALL
                     DFLLAEEARGQSVRLDEAAREALLAYPWPGNVRQLRNVLRTLCALCEHGVISHAELPA
                     EIRHARPALALLPAAKPDQLGDAERQALLAVLEVQHWQMSRSAEQLGISRNTLYRKLR
                     KHGIKLRSTL"
     gene            14335..15855
                     /locus_tag="VFF22_05210"
     CDS             14335..15855
                     /locus_tag="VFF22_05210"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZX16245.1"
                     /translation="MRYAHPGTEGALVSFKSRYGNYIGGEFVAPVKGQYFTNTSPVNG
                     LPIAEFPRSSAEDIDKALDAAHGAADAWGRTSVQERSLTLLKIADRIEANLEVLAITE
                     TWDNGKAVRETLNADIPLAADHFRYFAGCLRAQEGSAAEIDGNTVAYHIHEPLGVVGQ
                     IIPWNFPLLMAAWKLAPALAAGNCVVLKPAEQTPLGISVLMELIGDLLPPGVLNVVQG
                     FGKEAGEALATSKRIAKIAFTGSTPVGAHIMKCAAENIIPSTVELGGKSPNIFFEDIM
                     QAEPSFIEKAAEGLVLAFFNQGEVCTCPSRALVQESIYPAFMKEVMKKVEQIKRGDPL
                     DTDTMVGAQASEQQFDKILSYLEIAKQEGAELLTGGAVEKLSGTLDGGYYIQPTLLKG
                     HNKMRVFQEEIFGPVVSVTTFKDEAEALAIANDTEFGLGAGLWTRDINRAYRMGRAIK
                     AGRVWTNCYHLYPAHAAFGGYKKSGVGRETHKMMLDHYQQTKNLLVSYDINPLGFF"
     gene            16083..17531
                     /gene="eat"
                     /locus_tag="VFF22_05215"
     CDS             16083..17531
                     /gene="eat"
                     /locus_tag="VFF22_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015268800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine permease"
                     /protein_id="HZX16246.1"
                     /translation="MAAEQISAVGLPQGTDLEQVDADYFQSRQLKKGAAGWVLLVGLG
                     VAYVISGDYAGWNFGLAQGGWGGMFLATLLMALMYLCMCFSLAELSSMIPTAGGGYGF
                     ARSAFGPLGGFLTGTAILIEYAIAPAAIAVFIGAYCESLFGIGGWAIYLAFYVLFIGI
                     HIFGVGEALKLMFIITAVAAIALGVFIVAMVPHFSVANLLDIPVTEATGASSFLPFGY
                     VGVWAAIPYAIWFFLAVEGVPLAAEETRNPQRDMPRGLIGAMLVLLAFALLILVVGPG
                     GAGANALMASGNPLVEALTIAYGESTWMSSFVNLIGLAGLIASFFSIIYAYSRQIFAL
                     SRAGYLPRSLSLTNKNKAPVLALIVPGLIGFALSLTGQGDLLILVAVFGATISYVLMM
                     AAHITLRVRRPEMHRPYRTPGGIVTSGIALVLACVAVVAGFLVDPRVVIGAAVIYAVL
                     IAYFALYSRHHLVAGTPEEEFVAIGKAEAALK"
     gene            17598..18992
                     /locus_tag="VFF22_05220"
     CDS             17598..18992
                     /locus_tag="VFF22_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019818773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HZX16247.1"
                     /translation="MSSFQHTVGGEIYRFDSLAEVMAKASPARSGDFLAGVAASNAGE
                     RVAAQMALADIPLKHFLTEALIPYEVDEVTRLIIDSHDLAAFAPVSHLTVGGFRDWLL
                     GDSADEASLRALAPGLTPEMAAAVSKIMRVQDLVLVAQKIRVVSRFRNTVGLRGRMST
                     RLQPNHPTDEPAGIAASILDGLLYGNGDAMIGINPATDSISSICEMLKMLDGIIQRYE
                     IPTQACVLTHVTTSIEAINRGVPLDLVFQSIAGTEAANASFGVSLKILQEGYEAGLSL
                     KRGTLGDNLMYFETGQGSALSAGAHHGLDQQTCETRAYAVARHFKPFLVNTVVGFIGP
                     EYLYNGKQIIRAGLEDHFCGKLLGLPMGCDICYTNHAEADQDDMDVLLTLLGVAGINF
                     IMGIPGSDDVMLNYQTTSFHDALYARQSLGLKPAPEFEGWLQRMGIFTQADGRPRMGN
                     SLPPAFRQALAQLG"
     gene            19007..19819
                     /gene="eutC"
                     /locus_tag="VFF22_05225"
     CDS             19007..19819
                     /gene="eutC"
                     /locus_tag="VFF22_05225"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HZX16248.1"
                     /translation="MYDDSPLSGASENPWQQLRNLTPARIALGRAGTSMPTRAQLDFQ
                     FAHAQARDAVHLPFDHAALAAGLAHQHRQSLLLHSAAKDRDTYLQRPDLGRRLSHESA
                     ELLGQHRENNPDGYDLTIVIADGLSALAVHRNALPMVAHIEEQAAAEGWSVAPVALVS
                     QGRVAVADEVAELLGARMVVILIGERPGLSSPDSLGLYFTYAPRVGLTDAYRNCISNV
                     RQEGLSYAMASHRLLYLMREACRRQLSGVNLKDEAEVPLLAGGVQRENFLLG"
BASE COUNT         3769 a         6663 c         6106 g         3565 t
ORIGIN      
        1 tcgcggttgg catcgatggc attgcgcagc aggttgagca gcacctgctc cagcagaatc
       61 cggtcggcat acaccggcgg cagattgtcc gccagttgct cggcaatggt cacctgccag
      121 ttgctcgcct cccactgaca cagccgcaca gcctcacgcg ccacactggc gatatccagc
      181 gcctgggtgc gcctctggcc cttgcgtaga aaggcgcgca gacgcttgat cacttccgag
      241 gcgtggttgg cgtgctcgtt gatccgctcc aggccctggg ccaccttgtc cgccgcctgc
      301 ggattagtgc ccagggcctt gaggtagcgc tggctggcgc tggcgtagtt gaccaccgct
      361 gccagtggtt gattgatctc gtgagcgata ccggacgcca gctcgccaag ggtaaccaag
      421 cgcgcagtgt gcgccagctc atcctggtga cggcgctgct gagcctcgcg caattcgcgc
      481 tcggtcatgt cacgcgccac cagcgagtag taatgctcgc catcgcggcc gcggtgggcc
      541 agcagcacca gcgataccag caaaggcgca tgcgggccca gtggcaacag gcgcacttcg
      601 gcgctccaca cgccctggct gtcggcggtg ctccatccgc tacgttgcag acgtagcagg
      661 tcgtcttgcg ccagcagttg atcaagggcc ggcaatggct gcccgtcttc gatatgcagg
      721 gcatgccggg ccgacggatt aaggtgcgtc aggtgcccac tggggtcgat aaacagcacc
      781 aggtcggtat tggcctccac cacttcggcc agacgccgct tgttttcttc ggccagcacc
      841 cgcgcagtga tatcccgcga cacgctgacc acctcgacca ccgcgccggt ataggtttca
      901 cggatggcgc ggctggcagt ctcgaaccac aggtaatggc catcgcgatg acgaatgcga
      961 taggtcatgg tgtggtagcc atcctgctcc agggcctcgc gggtgctgtc caccagttgc
     1021 tgctgatcct gttcatggaa cagtgcctgc acatgggtgc cgcgcagggt ttccggccag
     1081 tagccaagca gcgtccagct ggccggcgaa gcgtcgagaa aacgcccatc cggggtgtgc
     1141 cgggaaatca ggtcggtggt gttttcagta atcaggcgat acagccggct ggccttggcg
     1201 gcttcacgct cggcctgcac ctgagcggtg gctggccggc agcgggccag cacctgctgt
     1261 tcggcagggt ccggcataaa gatccagatc agcacctggc cagcgtactg cgcttccaca
     1321 gcttcgatgg cgcggccttg ttgcaggcag gcgcgcacca gctgcggggt attcaccggt
     1381 agcagctcgg ccaccgccgc cagttcgcgc ggccccagca gttcatgcat ggcggcgttg
     1441 agctgcaacg ggcgcgcctg gatatccagc agcagactgg gctgcgggtc actgcccagt
     1501 aacaggctgc cacgggcctg ctgcggcaga ctggcgagca aggcctgcag caactgatga
     1561 accaaaccga gccaatcgct gctggcctgt tcctgcagct caatcagtag caaatcgcag
     1621 tgaccgtcat ccagcgccag ggccaatgcc tgcccgtgat tcagcccggc gcggcgcacc
     1681 cgtccggcca accagcaggg catctggcgc aacgcgccca ggtcgagcct cggctcatcc
     1741 tccagccgct cgcacagggc caggtcactg gcctgcagtg ggtcaccgaa cccgggcggc
     1801 aagtgcggcg cactgccctc atggctgtag atggccgtgc tcgcctgcca acgcaggaaa
     1861 tacgcctgac gcacttccgg gcaggcatgc aaggcgcgca atagtgcatc ggcgcgcacc
     1921 gccaggggca ggctttcacg ctgcatgcgc agccagctgt gcagctgcac agatatctca
     1981 ttcatccagg gtcattttcc ttgcatgccg atgctggcga gcatcagccc aagggtcacc
     2041 aattgcaaag cgaccatggg ctaagagtcg ttaaaacata tggtatatat actatacaac
     2101 tatggtttaa cttccatata aattcataca gatatataaa tagaaccaga acaataccgg
     2161 gccaccgcat aacactacct gcacaacggc ccgagcatcc aacctgccat gggtactcgc
     2221 atgtcgatct atgaacaggg ccttgcgcct actgccgtca accatatcgc cctctcgccg
     2281 ctgagcttta tcgagcgcac cgccagtgtt tacccacatt acccggccgt ggtgcatggt
     2341 tctattcggc gcaactggag tgagacctat gcgcgctgcc ggcgcctggc gtccgccttg
     2401 gccggccgcg gtatcggcaa gggcgacaca gtggcggtga tgctgccgaa catcccgcag
     2461 atgctcgaag cgcacttcgg cgtaccgatg atcggtgccg tactcaaccc gctcaacgtg
     2521 cgcctggatg ccgaggccat cgcctttatg ctgcagcacg gcgaagccaa ggtgctgatc
     2581 accgaccgcg aattccatga ggtgatccac gccgccgtcg gcatgctcga ccatccaccg
     2641 ctggtgatcg acgtcgatga cccggaatac ggcgaaggcc aggcggtcag cgatctcgac
     2701 tacgaggcct tcctcgccga aggtgacccg caatttgcct ggcagtggcc ggcagacgag
     2761 tggcaggcga tcagcctcaa ttacacctcc ggcaccaccg gcaaccccaa gggtgtggtc
     2821 taccaccacc gcggcgcgta cctgaactcc ctgggcaacc agatgacctg ggccatgggc
     2881 aaccacccgg tgtacctgtg gaccctgccg atgttccatt gcaacggctg gtgctacccc
     2941 tggaccatca ccgccctggc cggcgtgcat gtgttcctgc gccgcgtcga cccggcgaag
     3001 atcctcaccc tgatccgcgc cgagcaggtc acccacctgt gcggtgcgcc gattgtgctc
     3061 aatgccctgg tcaatatgcc ggccgaggcc aaggccgcca ttgatcatcc ggtcaaggcc
     3121 atggtcgccg gcgccgcacc accggccaag gtgattggtg ccgtcgagga aatgggtatc
     3181 cacgtcaccc acgtgtacgg cctgaccgag gtctacggcc cggtgaccct gtgcgcctgg
     3241 catgccgaat gggatgccct gccgctggat gaacgcgcgg tgatcaaggc gcgccagggc
     3301 gtgcgctacc cgaccctgga aggggtgatg gtggccgacc cgaaaaccct cgagccgacc
     3361 ccgcgtgatg gcgaaaccat cggcgagata tttatgcgcg gcaacaccgt gatgaagggc
     3421 tacctgaaga accccagtgc caccgccgaa gccttcgccg gtgactggtt ccacaccggc
     3481 gatctggcgg tgtgccaccc ggacggctac gtggaaatcc gcgaccggct caaggacatc
     3541 atcatttccg gcggcgaaaa catctccacc atcgaagtgg aaggcgtgct ctatcgccac
     3601 ccggccgtgc tcgaagccgc cgtagtcgcc cgcccggacg aaaaatgggg cgagaccccc
     3661 tgcgccttta tcaccctcaa ggccgaccaa cagcaagtcg ccgaggcgga catcatcagc
     3721 ttctgccgcg atcacctggc cggcttcaaa gtgccgaaaa ccgtggtgtt cacccagctg
     3781 ccgaaaacca gcaccggcaa gatccagaag tttgtcctgc gcgatatggc caaagccctc
     3841 tgaccagcat gtagcccgga tgaaatccgg ggacagcgat tcggattccc cggattgcaa
     3901 tccgggctac ggcaagaaca caccgtttgc acaacaacaa gaaccggccc tggcggccga
     3961 cggagaaccc ttatgcaaat tttcaagcgt cgcgacggcg atgaacaacc gcactggcct
     4021 gttggcccgt tcaaggtgcg cctgcccttt gtgcactacc gctgggaaac cgcggaaatg
     4081 ctccaggccc tgatcatgtt tgtggtcagc ctggcgatga ttcccctgct ggaaaaatac
     4141 ctcggcctgc cctacgacgt ggccctggcc tatgtggtgg tgtgcggcgt cggctttatg
     4201 ctgccggcgc tgctcggcgt gcccatggtg ccgggctgga ttacccccgg catccccgtg
     4261 gtgctgctgt ttctgggtaa tttcaaaccc ggcccggagg cgattcaggc gctgtttgcc
     4321 ctgcagtttc tggtgtttct gatcttcctg attctcggcg tcacgcgcct gggcagcacc
     4381 ctggtgcgct tgatccccaa ttcaatgaag ggcggcatca tcatcggtgc cggtatcgct
     4441 gcgctgatgg gcgagatatc caccggtggg cgactggcca acacgccaat ctccctggta
     4501 atcggcagcc tgatctgcct gtacctgatg ttctcggtgt cgttcaaagg cctcaccgaa
     4561 cgcctgccgc tggcccgcaa gatcgtcaac tacggcatgg tgccgggcat gctgatcgcc
     4621 atctttatcg gcattgccgt gggcgaatac aagatgcctg acgtgaagtt tggcatcacc
     4681 gcgccggcct tcggcgagat gtggaactac ctgccattca gcgtcggctt ccctggcctg
     4741 gacgtgttcc tgctggccgt gccgacggcg gtgattgcct acatcattgc cttcggcgac
     4801 atcatcgtcg gccagtcgct gatgcagcgc gctgatgaac tgcgcaccga cgaagtgatc
     4861 gagaacaaca tcgaccgcgt gcacctggtc accgccctgc gcaacgccct gcatgccttc
     4921 ttcgcccctt acccaggcct ggccggcccg ctgtggaccg ccgtcgcggc gaccatggcc
     4981 gaacgctaca agtacgggcg caaggccatg gactcgatct acagcggcgc gggcaccttc
     5041 tggatcaccg gctttatcgc cctgttcgcc ctgccgctgg tgagcttctt ccagccggta
     5101 ttgccgattg ccctgtcgct gaccctgatc ctcaccggct atatctgcct gatggtgggc
     5161 ttcgaacagc tgagcaacaa caccgagcgc ggcatcgccg gcaccatggg tgtggtgctg
     5221 gccgtctacg gtgcaggctg gggcctggcc gccggtgcgg cgctgtacat cctggtcgag
     5281 cgcacccacc tgctcaagtt caccactaaa acctccgtca aaggccaacc cgaggccgat
     5341 tgaccctacg gcccgtttcg gcgggccttt tttaacaccc gccgcgctct gcgcggcagc
     5401 tgtgcgagaa actgccgatg cctgagtaca acgccccgct gcgcgaccta cgcttcgtcc
     5461 tgcatgaagt cttcgatgct cccagcctgt gggctcgcct gcccgccctg gccgaaacgg
     5521 tcgatggcga aacggccgat gccattctgc aagaagccgc caaggtcacc ggtggcctga
     5581 tcgccccgct caaccgcagc ggtgatgaag aaggtgcgca gtggcacgcg ggcgaggtgc
     5641 gcaccccggc gggcttcaaa gaggcgtatg ccacttatat cgaaggcggc tgggtcgggc
     5701 tgtccggcaa cccaacctat ggcggcatgg gcatgcccaa gatgctcgcc gtggcatttg
     5761 aagaaatgct ctacgccgcc ggttccagct tcgccctgta ttccgcgctg agctcagggg
     5821 cctgcctagc catcgatgcc cacgccagcg aagcgctgaa aaacacctac ctgccgccga
     5881 tgtaccaagg ccgctgggcc ggctccatgt gcctgaccga ggcccatgcc ggcaccgacc
     5941 tggggctgat ccgcacccgg gccgaaccgc aggccgacgg cagctaccgc atcagtggca
     6001 gcaagatctt tatcaccggc ggcgagcagg acctgaccga aaatatcatc cacctggtgc
     6061 tggccaagct gcccgacgcc cctgccggcg cacggggcat ctcgctgttt ctggtgccca
     6121 aggtcatggt gaacaacgat ggcagcctcg gcgcgcgcaa cgccgtgagc tgcggctcca
     6181 tcgaacacaa gatgggcatc aaggcctcgg ctacctgcgt gatgaacttc gacggcgcca
     6241 ctggctacct gatcggcgag ccgaacaagg gcctggcggc gatgttcacc atgatgaatt
     6301 acgaacgcct gtccatcggc attcagggca tcggctgcgc cgaagcctcc taccaggcgg
     6361 cccgcgacta cgcctgcgaa cgcctgcaaa gccgcgccgc aacaggccca caagcgcagg
     6421 agaaagccgc cgacccgatc atcgtgcacg ccgatgtgcg gcgcatgctg ctaagcatga
     6481 aagccctgac cgaaggcggc cgggcgtttg cctgctatgt cggccagcaa ctggacctga
     6541 gcaagttcag cccggacgcc agcgagcgcc atgcagccga aaccctggtg gccctgctca
     6601 ctccggtggc caaggccttc ttcaccgaca ccgggctgga cagctgcgtg catggccagc
     6661 aggtgttcgg cggccacggc tatatccgcg agtggggcca ggaacagttg gtgcgcgacg
     6721 tgcgcatcgc ccaaatctat gagggcacca acggcatcca ggccctcgat ctgctcgggc
     6781 gcaaggtcat cgccaatggc ggcgtggcgc tgcgccagtt cactggggag gttcgccgct
     6841 ttgccgagca acccgacagc ccttatgccc ttgaactgct cgacgccgtg gagcgcctgg
     6901 agcgcaccag cagctggctg caggagcgcg ccgccggcaa tccgcacgaa accggcgccg
     6961 cctcggtgga gtacctgcac ctgttcggtt acaccgccta cgcctggatg tgggcgcgca
     7021 tggccgccgt ggcacagacc cggcgcgcag aggactaagc gttctacagc gccaaactgg
     7081 ccactgcagc gtttttcttc gcccgcctgc tgccgcgcac gctgggcctg gaggccagca
     7141 tccgcgccgg cagcgattcg ctgtacggcc tggccgccga acagttctaa gccgacaaca
     7201 ccgattgagc gtttgcgcgg tgcccgctct gggcgtcgcg cttttttatt cgaggcagag
     7261 gtcaagcatg caagaagtcg tcatagtcgc cgccacccgc accgccatcg gtgcctttgg
     7321 cggctcgttg agcagcatca gcgccgtcga cctgggcgca gccgtcatcc gccgcctgct
     7381 gaccgacact ggtctgccgg ccgaacaggt ggatgaagtg atcctcggcc aggtgctcac
     7441 cgccggtgcc gggcagaacc ctgcgcgaca gagcgtgatt cgcgccgggc tgccgcatac
     7501 cgtaccggca ttgaccctga acaaggtctg cggctccggg ctcaaggccg taatcatggc
     7561 cgcccaggcc atccgctgcg gtgatgccga ggtgatcatc gccggcggcc aggagaacat
     7621 gagcctctcg ccgcatgtcc tgcgcggctc acgcaccggc ctgcgcatgg gccacggcca
     7681 gttgctcgac agcatgatcc atgacggctt gtgggatgcc ttcaacgatt accacatggg
     7741 catcaccgcc gagaacctga ccgaacaata cggcatcagc cgcgccgaac aggatgcctt
     7801 cgccgcccgc tcacagcagc gcgcctgcgc cgcgattgcc gccgggcgct tcgctggcga
     7861 aatcaccccc atcgaaatcc cccagcgcaa gggtgacaac ctgatcgtcg ccgtcgatga
     7921 acaaccgcgt gccagcagca ccctggaagc cctgggcaaa ctgcgcccgg cattcaaaag
     7981 tgatggcagc gtcaccgccg gtaattcctc aacccttaac gacggtgcgg ccgccgtgct
     8041 gctgatgagc gccagtaagg ccaccgagct gaacctgcca gtgttggcgc ggatcaaggc
     8101 ccacgccagt gccggcgtcg acccggcgac catgggcatc ggcccggtat tcgccacctg
     8161 caaagccctg gaaaaggccg gttggagcct ggaacaacta gacctgatcg aagccaatga
     8221 agccttcgcc gcccaggcca tcgccgtcgg ccgcgaactg cactgggaca gcgccaaggt
     8281 caacgtcaac ggcggcgcca tcgcccttgg ccatcccatt ggcgcgtccg gctgtcgcat
     8341 tctggtcagc ctgatccatg agatgatccg ccgcgacgcc cataaaggcc tggccaccct
     8401 gtgcatcggc ggcggccagg gcgtagcgct ggcgatcgaa cgttaatctg aacggtggat
     8461 gtaaaaagcg acatccaccc tacgccgaca ttacccgtag ggtggatcac gcttcaccaa
     8521 tccaccctgc aatcccagac aagaacaaga gaatcaccat gcgaaccctg accaccctca
     8581 ccggcaatag ccagaagctc gacggcggcg ccatgttcgg caatgcgccc aaggccctgt
     8641 ggcagcgctg gatggcgccg gacgaactga accgcatcga cctgggctgc cgcgccctgc
     8701 tggtgcaaga ggatgagcgc aacatcctga tcgagaccgg catcggcgcc ttcttcagcc
     8761 ccgaactgaa acagcgcttt ggcgtccagg aagagcgtca tgtactgctc gacagcctgg
     8821 tcgcagtcgg cctgagcgat gccgatatcg acattgtgct gctcacccac ctgcacttcg
     8881 accacgctgg cgggctgctt gcggcctggg aggccgataa gcctgcgcgc ctgctgttcc
     8941 ccaatgcaca attcatcacc ggccaccgcc aatggcagcg cgcctgccag ccccacgccc
     9001 gcgaccgggc ctcctacatc ccggaactgc tggagttact ggaagccagc gggcgcctgc
     9061 aactgatcga tgaaacctcc agctgtgaac tgctcggcgc ggactggcgc ctgcactgga
     9121 gcgacggcca cacgcctggc caactcctgc cggaagtggc catgccggat ggtccggtgg
     9181 tgtttcctgg tgacctgatc cccggcgcgc cctgggtaca cctgccgata accatgggtt
     9241 acgaccgctt tcccgaaggc ctgatcgagg agaaacaggc gctgctaagc gacctgcagg
     9301 cccgcggcgg ccgcctggtg ttcacccacg acccggccgt ggccatgggc cgcgtggtgt
     9361 gcgatgacaa ggggcgttac ggtctggaag agacccaggc caatgtgcgt gagctggccg
     9421 aatagcttgg ccaaaaggcg gtgcgcacgg cgcaccctac gggatcgatg gtcgccgcgt
     9481 cgttgtaggg tgcgccatgc gcaccggccg gtcagagccc gccgctgaca ctcagccgca
     9541 agcccatggc ggtggccagg gacagcggca ataacagggt atcgagcagg gcgctggccg
     9601 gcaggtcgag ggccgcgtac ttcggggcct cgcagccgaa gcgctcgacg gcgcagcagc
     9661 caccctgcaa cgcatacagg tccagccggg taccggcata gaccaccgga gcaccaggct
     9721 tggccgcatt cagggtggcg actgtggcac agcccggcag cagcgccacg agcagcaggc
     9781 aagccacccg gtaggatggg ttagccgaag gcgtaaccca tgggcttttc gccctatcac
     9841 tcatcgccgc tgacctgatg atgctcgccc cagcgcggca gcatgtcctg ggggatattc
     9901 aggcagttga ggatgcgcgc cacgacgaaa tcgatcagat catcaatggt ctgcggctgg
     9961 tgataaaagc ccggcgccgc cggcaggata atcgcgccca tgttcgacag tttgagcatg
    10021 ttctccagat gaatgctgga gaacggtgcc tcgcgcggca ccagaatcaa ttgacgacgc
    10081 tccttgaggg ccacgtcggc ggcgcgctca ataaggttgt tgcaggcgcc attggcaatc
    10141 gccgagagtg agccggagct gcacggcacc accaccatcg cagtgggcgc acccgagcca
    10201 gaggccgccg gagccatcca gtcttccttg ccgtacacgc gaatctgccc cggcgcggcg
    10261 ccggtgtact cgctcaagaa cgcctgcatg gcctggggtt tgttcggcag gctgacatcg
    10321 gtttcggtgg ccatcaccag ctgcgcggcc ttggaaatca gaaagtgcac ctcgcgctct
    10381 tcctgcacca gacagtcgag caggcgcagg ccgtactgag cgccggaggc gccggtcatg
    10441 gccaaggtaa tacgttcagg accgctcatg cgaactccat gggatgggtg aaaccgtcgg
    10501 gcgtaggatg ggttgagcgc agcgataccc atcaccatag cctcgatggg tatcggcgct
    10561 gcgcgcctca acccatccta cagccttacc taatctgcgc tcgcgacgct ttgagcgcga
    10621 attttggctc tttataaaag ggccgctgcc agcttgccat gcaaaccgcc aaagccgccg
    10681 ttgctcatca ccactacctg ggtgcccggc gcggcctcgg ccttgacccc agcaatgatc
    10741 gcctccaggg agtcgcagac ctgagttggc acggtcgagg tggccacggt gccggccagg
    10801 tcccaaccga ggttcggcgg tgcgtaccag tacaccgaat cggcctgcac tacggactcc
    10861 gacagcccat cgcggtgcgc gccaagcttc atcgagttgg agcgcggctc gacgatggcg
    10921 atcaatttgg cgtcgcctat gcgtttacgc aggccatcca gggtggtggc aattgccgtc
    10981 ggatggtggg cgaagtcgtc gaagatggtc acgccattga cttcagcgac cttctccata
    11041 cgccgcttgg cgttgataaa gccgcacagc gcagcgatgc ccagctccgg taccacaccg
    11101 acatgccgcg ctgcggccag caccgccagg gcgttggcta cgttgtgctg gccagtcagt
    11161 tgccagtcca ctgtacctgc ggttttaccg tcgaaactga cctcaaagcg cgagccgtcg
    11221 gcgctaagca ggcgcgcctg ccattggccg ccgtcaccag tggtctgcac cggggtccag
    11281 caacccatct cgattacccg cttaagcgcg ggctcggtgg tcggatgaat gatcaggccg
    11341 tcgcccggaa tggtgcgcac cagatggtgg aattgccgct cgatggccgc cagatccggg
    11401 aagatgtctg cgtgatcgaa ttcaagattg ttcaggatcg cggtgcgcgg gcggtagtga
    11461 acgaacttgc tgcgcttgtc gaagaaggca ctgtcgtact cgtcggcctc gaccacaaag
    11521 aacggcgtgc cgcccaagcg cgcggaaata ccaaagttct gcggcacgcc gccaatcaga
    11581 aagcccgggc tcatgccggc atgttccagc acccaggcca gcatgctcga gctgctggtc
    11641 ttgccgtggg taccggccac cgccatcacc cagcggccct gcagcacgtg gtcggccaac
    11701 cactgcgggc cgctcacgta aggcaggcct ttgttcagca catattccac cgccgggttg
    11761 ccacgcgaca gggcgttacc aaccaccacc aagtccggcg cggggtcgag ttgggcggcg
    11821 tcatagccct gggtcagttc gatgccctga gcctccaact gggtgctcat aggcgggtaa
    11881 acgttggcgt cagagccagt cacgcggtgg ccgagttcct tggccagcac ggccagcgag
    11941 cccataaaag tgccgcagat gccgagaata tggatatgca tgatttacct tgaaggcgtc
    12001 gtcagagccg gtgaataagg cgggataaaa actggccgca gattagcaca gcgccgccgc
    12061 cccgcagagg cctggcggcg cgctgcgcgg cagggctcaa gaggcatgat tcaaagcgat
    12121 agttcaaagc gttgagcgca gcttgatgcc gtgtttacgc aacttgcggt acagggtgtt
    12181 gcggctgatg cccagctgtt cggcgctgcg gctcatctgc cagtgctgca cctctaggac
    12241 cgcgagcaag gcctggcgtt cggcatcacc cagctggtcg ggtttggccg caggcaacag
    12301 cgccagcgca ggacgggcat ggcgaatttc ggcgggcagc tcggcgtggc tgattacgcc
    12361 gtgttcgcaa agcgcgcaaa gggtgcgcag cacattgcgc agctggcgca cattacccgg
    12421 ccaggggtag gcgagcagcg cctcgcgggc agcctcatca agccgcacag attgcccgcg
    12481 cgcctcttcg gccaggagaa aatccagcaa tgccagcttg tcgctgcgct cacgcaaagg
    12541 cggcagttcg atcaccaggc cattgaggcg atagaacaga tcctggcgaa actcgccgtt
    12601 ggccactcgt tcgttaaggt cgcggtgact ggcactgatc acccgcacat cgatggcctg
    12661 ggcttcaccg ccaatcggct cgacccggcg ttcttccagc acccgcagca aacgggtttg
    12721 cagcgccagc ggcatatcgc cgatctcatc gagaaacagc gtgccgccgt cggcctgctg
    12781 cagcttgccg cgcatgcctt ccttgcgcgc gccggtaaag ctgccgccgc gatagccgaa
    12841 cagttcactc tcgatcaagc tctcgggaat tgccgcgcag ttcagcgcaa caaagggctt
    12901 gccgccacgc gagctgacct gatgcagcgc cttggcgaag gcctccttgc cggtgccggt
    12961 ttcgccgccg atcagcagcg gcacatcacg ctcatacacc cgcagggcac ggcggaaatc
    13021 actctgcaaa ccggcgtcgg ccaggcacag ccctggcgtt gccaagctcc ctgcagccag
    13081 tgcctgggcg ctgcgccgag gctggccgcg cagcatggca aacagcagct gcccgcgctg
    13141 ggtatacagc ggccaactgg cactgggctg cgccgaggcg cgaccgagta aatcgtccag
    13201 acggcaatca aacacctcga tcaaggtgcg cccgagcagt tgccggcgcg acagcccgag
    13261 caagttgatc gcactctgat taactgcact gacccggcca tcgccatcga aggccagcag
    13321 cccttcgctg aactggccga tatattcagc ctgggcatgg aagcgcagca gccattcacc
    13381 ttcatagctg cgcaggaaat ggcagccctc gatggctttg gccgacaggt tgaccagggc
    13441 catggtgtgg aactggctct gccgcgatac gtcatgccgc gccgaggaca catctagcac
    13501 cgccagcaac tcgccatgcg gatcgaaaac cgggctggcc gagcaagtca gaccggtatg
    13561 ccggctgcgg aaatgttcat tctggtggat ggtcagcggc tggcgctcga ccaggcaggt
    13621 gccaatgcca ttggtgcctt cgcaggcttc gctccaatcg gcaccaagcc acagcccggc
    13681 ttgctcgaag gtgcgttttt ccgcctgctc ggtgacgcag ttgaggatca ccccacgcgc
    13741 atcagtcagc agcaccgcgt gaccgctgcc ggccagctgg cggtgcaggc tgttcatctc
    13801 gttactggaa atctccagta cctgttgcag ctgctcgcgc cgttccagca tatgcgcgtg
    13861 ctcgatcacc gccggcggca tgctctgcgc cggatcgagg tgatgctgct gcagacaacg
    13921 cagccaggag cgcgccaccg aagaatcggc agccgggccg gcgagttgtc cacggccctg
    13981 ggccacggtc agcacctgct gggcgtggcg gcttgcaggg ttgtgcattg ttattgttct
    14041 ccactctagg agttttgcac gtgatggccg agcatctacc agctttccgg ccattgcaag
    14101 acgcgctgac cagcgagtca ccatgctgta tcgcccctgg tacagagtgt cacgcaggct
    14161 gtatcagggc cgatacagtc ctcgcgctac aaccttcaac cgaaacccac gcaaccgctc
    14221 tagatcgaga gtcacgcacg cctggcccag cccttgctct gtctctgcac aagcgccaca
    14281 gcgcgccgtt cgctgcatca ccagaacaag cataagaacc aggagatatt cagcatgcgt
    14341 tacgcccatc ccggtaccga aggtgccctc gtttcgttca aatcccgtta cggcaactac
    14401 atcggcggcg agtttgtcgc gccggtcaaa ggccagtact tcaccaacac ctcgccggtg
    14461 aacggcctgc cgattgccga gttcccacgc tccagcgccg aggatatcga caaggcgctg
    14521 gacgccgccc atggcgctgc cgacgcctgg ggccgcactt cggtgcagga gcgctcgctg
    14581 accctgctga agatcgccga ccgcatcgaa gccaatctgg aagtgctggc catcaccgaa
    14641 acctgggaca acggcaaggc cgtacgtgaa accctcaacg ccgatattcc cctggccgcc
    14701 gaccatttcc gctacttcgc cggctgtctg cgcgcccagg aaggcagcgc tgccgagatc
    14761 gacggcaaca ccgtggccta tcacatccat gaaccgctgg gcgtggtcgg gcagatcatc
    14821 ccgtggaact tcccgctgct gatggccgcc tggaaacttg ccccggccct ggccgccggc
    14881 aactgcgtgg tgctcaagcc tgccgagcaa accccgctgg gcatcagcgt gctgatggag
    14941 ctaatcggcg acctgctgcc gcccggcgtg ctcaacgtgg tgcaaggttt tggcaaggaa
    15001 gccggcgaag ctctggccac cagcaaacgc atcgccaaaa tcgccttcac cggctccacc
    15061 ccggtgggcg cgcacataat gaaatgcgcc gccgaaaaca tcatccccag caccgtggaa
    15121 ctgggcggca aatcgccgaa tatcttcttc gaagacatca tgcaggccga gccgagcttt
    15181 atcgaaaaag ccgccgaagg tctggttctg gcgttcttca accaaggcga ggtgtgcacc
    15241 tgcccatcgc gagcgctggt gcaggaatcg atctacccgg cctttatgaa agaggtgatg
    15301 aagaaggtcg agcagatcaa acgcggcgac ccgctagaca ccgacaccat ggtcggcgcg
    15361 caagcctccg agcagcagtt cgacaagatc ttgagctacc tggaaatcgc caagcaggaa
    15421 ggcgctgaac tgctgaccgg cggcgcggtg gaaaaactca gcggcaccct ggacggcggc
    15481 tattacatcc agccgaccct gctcaagggc cacaacaaga tgcgcgtgtt ccaggaggag
    15541 atcttcggcc cggtggtcag cgtcaccacc ttcaaggacg aagctgaagc cctcgcaatt
    15601 gccaacgaca ccgagttcgg cctcggtgcc ggcctgtgga cccgcgatat caaccgcgcc
    15661 taccgcatgg gccgggcgat caaggccggg cgcgtgtgga ccaactgcta ccacctctac
    15721 ccggcgcatg ccgccttcgg tggctacaag aagtccggcg ttggccgtga aacccacaag
    15781 atgatgctcg accactacca gcagaccaag aacctgctgg tgagctacga catcaacccg
    15841 ctgggctttt tctaactcgc ctaaatggtg gcgggttgca cccgccctac gggctcgaaa
    15901 cagcctgcgc aaccgcagcc gatgctcgga aacgacatcg gcctgccagc ctgcgcccca
    15961 ggtctagacc agaggcgcag ttggcacaac ggttgctgat ccactgggta acactcttcc
    16021 ctcaaatagc tacacgcctg gccgggcctg cgctgcggcc agcgtgttga taaggaattc
    16081 ccatggcagc agaacaaatc agtgcggtag gtctaccgca aggcaccgat ttagagcagg
    16141 tcgatgccga ttacttccaa agcagacaat tgaagaaggg cgcagctggc tgggtattgc
    16201 tggtcggcct gggcgttgcc tacgtgattt ccggcgatta tgccggctgg aacttcggtc
    16261 tagcccaggg cggctggggc ggcatgtttc tcgccaccct gctgatggcg ttgatgtacc
    16321 tgtgcatgtg cttttccctg gcagaactgt cctcgatgat ccccaccgct ggcggcggct
    16381 acggctttgc ccgcagtgcc ttcggcccct taggcggctt cctcaccggc acagcgatcc
    16441 tcatcgagta cgccattgcc ccagcagcga ttgcggtgtt tatcggcgcc tactgcgagt
    16501 cgctattcgg cattggcggc tgggcgatct acctagcctt ctatgtgctg tttatcggca
    16561 tccatatctt cggcgtcggt gaggcgctga agctgatgtt tatcatcacc gccgtggcgg
    16621 cgattgccct cggcgtgttt atcgtcgcga tggtgccgca cttctcggtg gccaacctgc
    16681 tcgatattcc ggtgaccgaa gcaaccggcg ccagcagctt cctgccgttc ggctatgtcg
    16741 gcgtgtgggc ggcgatcccc tatgcgatct ggttcttcct cgcggtcgaa ggcgtgccgc
    16801 tggccgctga agaaacccgc aacccacagc gcgacatgcc acgcgggctg attggcgcga
    16861 tgctggtgct gctggccttt gccctgctga ttctggtggt gggcccaggc ggtgccggtg
    16921 ccaacgcgct aatggcctcc ggcaacccgc tggtggaagc cctgaccatc gcttacggcg
    16981 aatccacctg gatgagcagc ttcgtcaacc tgatcggcct ggccggtctg attgccagct
    17041 tcttctcgat catctacgcc tactcccggc agatcttcgc cctgtcgcgc gccggctacc
    17101 tgccgcgttc gctgtcgctg accaacaaga acaaggcccc ggtactggcc ttgatcgtgc
    17161 ccggcctgat cggctttgcc ctgtcgctga ctggccaggg tgacctgctg attctggtgg
    17221 cagtgttcgg cgccaccatc tcctacgtgt tgatgatggc cgcacacatc accctgcgcg
    17281 tgcgtcgtcc ggagatgcat cgcccgtatc gcactccagg cggcatcgtc acctcgggca
    17341 tcgccctggt actggcttgc gtggccgtgg ttgccggttt cctggtcgat ccacgggtgg
    17401 tgattggcgc tgcggttatc tacgccgtgc tgatagccta cttcgccctg tacagccgcc
    17461 atcacctggt ggccggtacg ccggaagaag aattcgtcgc catcggtaaa gccgaggccg
    17521 cgctcaagta gccaagtaac caagtaacac cgccgcgcag gcgcaactgc gctagcgcgg
    17581 cgtttgggag acacgcgatg tccagttttc agcatacggt aggcggtgaa atctaccgtt
    17641 tcgacagcct cgccgaggtt atggccaagg ccagcccggc gcgctccggc gactttctcg
    17701 ccggtgtggc ggcgagcaat gccggcgaac gggtcgcggc gcaaatggcc ctggccgata
    17761 ttccgcttaa acacttcctc accgaggcgc tgattcccta cgaggttgat gaagtcaccc
    17821 ggctgattat cgacagccat gacctggcag ccttcgcccc ggtcagccac ctcaccgtcg
    17881 gcggctttcg cgactggctg ctcggcgaca gcgccgacga agccagcctg cgcgccctcg
    17941 cccccggcct gacgccggaa atggccgctg cggtatcgaa gatcatgcgc gtgcaggatc
    18001 tggtgctggt ggcgcagaaa atccgcgtgg tcagccgctt tcgcaacacc gtgggcctgc
    18061 gcgggcgcat gtccacccgc ctgcaaccga atcacccgac cgatgagccg gccggcatcg
    18121 ccgcgagcat tctcgacggc ctgctgtacg gcaacggcga cgccatgatc ggcatcaacc
    18181 cggccaccga cagcatcagt tcgatctgcg agatgctgaa gatgctcgac ggcatcattc
    18241 agcgttatga aatccccacc caggcctgcg tgctgaccca cgtcaccacc tcgatcgagg
    18301 cgatcaaccg gggggtgccg ctggacctgg tattccagtc gattgccggc accgaggcgg
    18361 ccaacgccag cttcggcgtc agcctgaaga ttcttcagga aggctatgaa gccggcctgt
    18421 cgctgaaacg cggcactctg ggcgacaacc tgatgtattt cgaaacaggc cagggcagcg
    18481 cgctgtccgc cggtgctcac catggcctcg accaacagac gtgcgagacc cgcgcctatg
    18541 cagtagcgcg gcatttcaag ccgtttctgg tgaataccgt ggtcggcttt atcggtccgg
    18601 agtacctgta taacggcaag cagatcatcc gcgccggcct ggaagaccac ttctgcggca
    18661 agctgctggg cctgccgatg ggctgcgaca tctgctacac caaccacgcc gaagccgatc
    18721 aggacgacat ggacgtgctg ctgactctgc ttggcgtggc cggcatcaac ttcatcatgg
    18781 gcatccccgg ctccgacgac gtgatgctca attaccagac cacctccttc cacgacgcgc
    18841 tgtatgcccg gcagagcctg gggctgaaac cggccccgga gttcgaaggc tggctgcagc
    18901 gcatgggtat cttcacccag gccgatggcc gcccgcgcat gggcaacagc ctgccgccgg
    18961 cgtttcgtca ggcgctggcg cagcttggtt aactaaggag ccgcttatgt acgatgacag
    19021 cccactcagc ggcgccagcg aaaacccctg gcagcaactg cgcaacctca ccccggcgcg
    19081 tatcgccctg ggtcgtgccg gcaccagcat gccgacccgt gcgcagctgg atttccagtt
    19141 cgcccacgcc caggctcgcg atgccgtgca cctgccgttt gatcacgccg cactggccgc
    19201 aggcctggct caccagcatc ggcagagcct gctgctgcac agcgccgcca aggatcgcga
    19261 cacctacctg caacgcccgg atctgggtcg gcgcctaagt cacgaatccg ccgaactgct
    19321 ggggcagcac cgcgagaaca atccggatgg ctacgacctg accattgtca tcgccgatgg
    19381 tctgtctgcc ttggccgtgc accgtaacgc cctgcccatg gtggcgcata tcgaggaaca
    19441 ggccgctgcc gaaggctgga gcgtggcgcc cgtcgccctg gtcagccaag gccgcgtggc
    19501 ggtggccgat gaagtggccg aactgctcgg cgcacgcatg gtggtgatcc tgatcggcga
    19561 gcggccaggc ctaagctcac cggacagcct ggggctgtat ttcacctacg ccccgcgggt
    19621 cggcctgacc gacgcctacc gtaactgcat ctccaacgtg cgccaggaag gcctgagcta
    19681 cgccatggcc agccatcgcc tgctgtacct gatgcgcgag gcctgccgcc gtcaactgtc
    19741 gggggtcaac ctcaaggatg aagctgaagt gccgctgctg gccgggggag tgcaacgcga
    19801 gaacttctta ctgggctaag aacctgctta cgatcagctg cgcgtcggcc ctgctgcgtt
    19861 taaaaccggc tcggagtgct catttacacc agtaaactcc gcttcctcgc cggttttcgc
    19921 cttgcatggc tctagctcgc aagatcgaaa gcagattccg aggggaaatt tccgcaaact
    19981 acaaccaaac gcttgtttgg attgcacttt ccaaaagctt taggcagcat cggggtcgtt
    20041 cgcaggcagc gctgccgtgt tgccgaagcc atccacatga ctttaagtag aggctcacca
    20101 tgc
//
LOCUS       DASRUM010000074        25848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_539787, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000074 DASRUM010000000
VERSION     DASRUM010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 25848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25848
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539787"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..608)
                     /gene="sixA"
                     /locus_tag="VFF22_05230"
     CDS             complement(144..608)
                     /gene="sixA"
                     /locus_tag="VFF22_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004576957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HZX16249.1"
                     /translation="MKLWLLRHGEAEPTARTDAERNLTAYGRQQVREAAAQLLGRPLQ
                     AVLVSPYVRAQQTADLACDVLAFSGIRQTVPWLTPESDLRQALRELDGYAFDELLLVT
                     HQPLVGALAGLLIHGHRQQPLPMNTASLALLEGDELAAGLMELSAHKHPSRS"
     gene            complement(634..981)
                     /locus_tag="VFF22_05235"
     CDS             complement(634..981)
                     /locus_tag="VFF22_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HZX16250.1"
                     /translation="MSNDNQELQRESILLRILWMVIFTIVWQLAEILLGAVVLLQLGY
                     RLFYGAPNAGLLGFGDSLSQYLAQIGRFGTFNSDEKPWPFADWPTPQAPQGETPHSIP
                     PAAHPVRDEEPKL"
     gene            complement(1016..2038)
                     /locus_tag="VFF22_05240"
     CDS             complement(1016..2038)
                     /locus_tag="VFF22_05240"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HZX16251.1"
                     /translation="MTDQQPIAVLGGGSFGTAIANLLAENGQQVLHWMRDPQQAQAIR
                     LTRENPRYLKGIKIHPGVDPVTDLAATLNACQLVFVALPSSALRQALQPVAELLSGKL
                     LVSTTKGIEAQSFLLMSQILEDIAPQARIGVLSWPNLAREVAEHALTASVVASEDEDL
                     CLQVQAALHGRTFRVYASGDRFGVELGGALKNVYAIIAGMAAAMGMGENTKSMLITRA
                     LAEMTRFAVKLGANPMTFLGLAGVGDLIVTCSSAKSRNYQVGFALGEGLSLEDAVERL
                     GEVAEGVNTIKVLKAKAEELQVYMPLVTGLHAILFEGRTLDQVIGALMNAEPKTDVDF
                     IPTTGF"
     gene            2289..4211
                     /locus_tag="VFF22_05245"
     CDS             2289..4211
                     /locus_tag="VFF22_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX16252.1"
                     /translation="MILRRGWNIHTRTQLISVGPALLLTLLLTGFFTFARLQDLRQEL
                     NHNGQLIASQLAPASTYGVISGNLDVLDALLQATLKTPHVRFLEVRDHTGKILVYVEQ
                     TPGSGHASSQVEVFQAPIHLQRAALNNSTQQTLTDDYLGYVVVGMSNDAFNTRQQEIL
                     FKATLLALFALVLTFILARRLARHLSQPLSAMGEAVSAIQAGNYQTTLPEVGEGELQA
                     LARHINNLAHGLASASEEQQRAMALLIQAREQSELANRAKSDFLAMMSHELRTPMNGV
                     LGMLQLLETTEMTQEQAEYAALATESTEHLLKVINDILDFSRIERGALELERIPFSLL
                     ELLQSSIQVFQHSAQQRGLQLLLETQSGLEQLQVQGDPTRIRQILVNLIGNALKFTET
                     GSIRVHSTWQALDNQVLWFSCSVHDSGIGISPERLEHMFDAFQQADTSISRRYGGTGL
                     GLPIARTLAERMGGTLRAVSQEGEGSVFTLEVPLPFSQHVAPAVSDTSLPADQGNGQP
                     VLLVEDNPVNQTVIEAMLRSLGYQVSLVGDGAQAVHQVSQQHYAAILMDCRLPIMDGY
                     EATRQIRRLPGCAELPIIALTANALQGDREACLQSGMNDYLAKPFKRADLQRILQRWL
                     PPRPSTGSQEGSTAAG"
     gene            4386..4901
                     /gene="fabA"
                     /locus_tag="VFF22_05250"
     CDS             4386..4901
                     /gene="fabA"
                     /locus_tag="VFF22_05250"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HZX16253.1"
                     /translation="MTKQNAFTREELLACGRGELFGPGNAQLPAPNMLMIDRITHISD
                     TGGKYGKGEIVAELDITPDLWFFACHFEGDPVMPGCLGLDAMWQLVGFHLGWQGNPGR
                     GRALGSGEVKFFGQVLPTAKKVTYNIHIKRTIARSLVLAIADGTVSVDGREIYSAEGL
                     RVGLFTSTDNF"
     gene            4914..6134
                     /gene="fabB"
                     /locus_tag="VFF22_05255"
     CDS             4914..6134
                     /gene="fabB"
                     /locus_tag="VFF22_05255"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HZX16254.1"
                     /translation="MRRVVITGLGIVSCLGNDKATVSANLRAGKSGIRFNPAYAEMGL
                     RSQVSGSVDLNLEELIDRKVYRFMGDAAAFAYLSMEQAIKDSGLSAEEISNPRVGLIA
                     GSGGASTFNQMEAMDILREKGVKRVGPYRVPRTMGSTVSACLATPFKIKGVNYSISSA
                     CATSAHCIGNAMEQIQLGKQDVVFAGGGEEEHWSQSFLFDAMGALSTQYNETPEKASR
                     AYDAKRDGFVIAGGGGMVVVEELEHALKRGAKIYAEIVGYGATSDGYDMVAPSGEGAV
                     RCMQQALATVDTPIDYLNTHGTSTPVGDVAESRAVREVFGAKAPAISSTKSLSGHSLG
                     AAGVQEAIYCMLMMEGNFIAGSVNIDEVDPEIADLPIQRSTVENAKLDTVMSNSFGFG
                     GTNATLVMKRWAGK"
     gene            complement(6213..7838)
                     /locus_tag="VFF22_05260"
     CDS             complement(6213..7838)
                     /locus_tag="VFF22_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003223037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX16255.1"
                     /translation="MTLRNLNIAPRASLGFGLVALLVLILGSFSLLQMAEMHKQSQQV
                     NENWLPSILNLGEMNQDILRLRALTLRLMLNTDEGMTRQNLARLEELRGVLNKAQDGY
                     EKLIASEGERLIYQRFKQAESSYLQEQSKIVEAVRQSDLDTALAVANGPLNGHADSMA
                     KALVELSELNRVGATAAADSAEAVFVSSRVWVIVMIALALGLTVILALLLTRSIVAPL
                     TEAVRVAEVVASGNLTQSIEVQGSDEPARLLGALKSMQQSLRSTIQNISDSSTQLASA
                     SEELSAVTEDSTRGLHQQNNEIEQAATAVNQMTSAVEEVARNAVSTSEASSESNRTAQ
                     HGREQVRQTVDSISHLADDVTNTASEVEQLAGQVRDISKVLDVIRSIAEQTNLLALNA
                     AIEAARAGEAGRGFAVVADEVRALAHRTQQSTREIEQMVSGIQQGTDKAVNAMQNSNS
                     RARSTLDVAKAAGLALDEIAAAISQINERNLVIASASEEQAQVAREVDRNLVNIRDLS
                     MQSSAGANQTSAASQELSRLAVDLNALVARFSI"
     gene            complement(8022..8486)
                     /locus_tag="VFF22_05265"
     CDS             complement(8022..8486)
                     /locus_tag="VFF22_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3859 domain-containing protein"
                     /protein_id="HZX16256.1"
                     /translation="MQYTRFSALAALVLTSSLAQAEVRVEGPVEYGVFASDYQDFQEG
                     ERVLTRSNQQIEQGEVIPAKLGTKFGLRYSLAGKMADDSPLTLLYLTPGVVTPQGTRH
                     DKFVVIQKLVPGAPQDVMAFEFTEKHEVVPGEWHFMVFQDDRKLLEQRFTVR"
     gene            complement(8549..9577)
                     /locus_tag="VFF22_05270"
     CDS             complement(8549..9577)
                     /locus_tag="VFF22_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZX16257.1"
                     /translation="MPLPVSRMSIALLLGLLSSMAQARDYRYSDAHLHYVDFFQESAG
                     MTELFEQMAAGNIEHVMLSGIPVAKKWHEDEPKRPRYYAGDDANAYWYSATDVLVAHA
                     YKQVPAEQRKRLHPFLSGFNPNDKNADAHIRRMLELDPGLWQGIGEIFTRHDDLTALI
                     HGDAPRANNEALARVFHLAAEYDLPVMLHSNITSKRERNPLYLQEIEDPLRNHPHVRF
                     IWAHAGTSMEIHRHQEKLDFLLPTLERMLKQYPNLYIDLSWTVLQPYLLDADGKPDPA
                     WVKLVSSYPARFMLGSDVVGRFTSLADYMSGFKPFLDALPEDVAHQVARDNFLAVLPR
                     RVQAELAE"
     gene            9804..11198
                     /locus_tag="VFF22_05275"
     CDS             9804..11198
                     /locus_tag="VFF22_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX16258.1"
                     /translation="MLQLLRSTPRKNTDDIALDALFNQHDLTARLSDSYRWMSRLNDF
                     SAGLHQRIRTSLGAAVGIASHAPQLARIAAANQQSGQTLAQSSELIASASEQVTTTLD
                     AELVPGAAEVAQLSAEVTATLHDCQQSGQAVLQQVEVIGKSEQQLELVIQQLTSQLEE
                     VSKVIGVIASISQQTNLLALNAAIEAARAGEHGRGFAVVAEEVRRLAGHTTDATTQVS
                     DIIERFRSGMQQLTEAGSHMHQAVADGRDGMLAVGGGLDSTRQAMDRLDRRVGQIAAG
                     TEQIGQAVRSINGDVQNIARVAGELLGKASQVLHHSQAVREDGDRLLAGLGDFRLEIH
                     HAVQASVEQLARQPGMSADVATAERLLGDTLKRDARFELFYLVDSRGVQLSENIFATD
                     VNHSESSSCKGRNWQQRPWFRAAADSLCSHITQVYRSSATDDFCFTVSVPILDARGQL
                     LRVLGADVRLSALV"
     gene            11255..11653
                     /locus_tag="VFF22_05280"
     CDS             11255..11653
                     /locus_tag="VFF22_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZX16259.1"
                     /translation="MISISNEKGLLQRISIGPHTLHSDVSVELGGEGAGPEPHDLFDA
                     ALGACKALTLALYAKQRGLPLEGLDVKLNRDDSQERQGIYRLDVELTLHGALDDAQRQ
                     QLLRVADKCPIHKLMTSSEVQVSTQLSEGA"
     gene            11653..12513
                     /locus_tag="VFF22_05285"
     CDS             11653..12513
                     /locus_tag="VFF22_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HZX16260.1"
                     /translation="MNDFLLIQPRPENLGGQPILRPLPSAKCRSVGPFVFFDHILENT
                     YPAGSGMNINQHPHIGLSTLTYLFKGQLQHKDSLGSNQLVSPGEVSWMTAGRAVAHVE
                     RTPQTLWESGSHMHGLQVWLAMPTAEEQGEPSYSHHPADSLPRSASMGVDICLIAGTG
                     FCLQSPVPVLSPTIYAEIKLAAGSALQIPAEHAERALYLIEGEATLDDQQLSLRGLLV
                     LPEGETYTLSACSESHLVLIGGAPIGPRRINWNFVASDPALIDQARQRWAAEDWPTVL
                     GETERIELPR"
     gene            12604..13152
                     /locus_tag="VFF22_05290"
     CDS             12604..13152
                     /locus_tag="VFF22_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZX16261.1"
                     /translation="MAQPLFDYESHLEACARQDDRAFQALYQQEAAQMLGLAITLLGR
                     RDQAEDCVHDAFVQIWRNAQRFQRALGSGRAWIYSILRYRALNQLRSRGRTVELDDDF
                     IERAADDSPQAAELLEQQGEQQQLRRCLQSIERNRRHPILLAFYRGLTHEQIANRLTT
                     PLGTIKGRIRAGLRALQECLQA"
     gene            13149..13862
                     /locus_tag="VFF22_05295"
     CDS             13149..13862
                     /locus_tag="VFF22_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HZX16262.1"
                     /translation="MIPQQPAERRALIGEYVLGLLEEAEAAEVRELIEQDAEAAQMAL
                     QWQQHFLELSDQLPPQTPSAQLWQRLQDSLRLTPAVSRVNSWLTWWQSLTTWRLTSAA
                     LAFALIVALMPNLWRTDMAGNSYTVVLQAPGEAAKPGWVVHVDGSGSLRLQPLLEDQI
                     PADRSVQFWTLIDPAIGPRSLGLVEPGKSLQLSAEQIGAVQAGQLFELTLEPAGGSPK
                     DRPTGKVLYIGRAVMASVN"
     gene            14017..14808
                     /locus_tag="VFF22_05300"
     CDS             14017..14808
                     /locus_tag="VFF22_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4394 domain-containing protein"
                     /protein_id="HZX16263.1"
                     /translation="MNSLNTLLTAAVATATLSLAAGTASAATLLALGADGQLFKIDSA
                     SQKITASMPVSSPSPLRGIDVRPANGMLYALGSDNQLYTLDLATGAASKVASLNKQLP
                     GSGQAVIDFNPVADKLRMLAADGTSFRVNVETGEVVVDGSVAYAAESPYAGKSAQVVA
                     GAYNNAYAGTQATALYNVDLASSSLMLQNPPNDGIQQAVGKIVDGLQNAALDIASDGK
                     GGNTAYLLTGTTLHTLDLATGKPTTLGEIADLPGNIIDIAVLPNM"
     gene            complement(14850..15632)
                     /locus_tag="VFF22_05305"
     CDS             complement(14850..15632)
                     /locus_tag="VFF22_05305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZX16264.1"
                     /translation="MKKSLLPLALAGLVGVAEAAVVVKPVPYEIDGEAFEGLVVYDDA
                     VTTPRPGLLMVPNWMGVNENAATKAALLAADKYVIFVADLYGKAIRPTNADEAKVAAT
                     TVRSDRPLMRKRAQEAVEVLKAQAGQVALDTARLGAIGFCFGGGAVLELARSGADLKG
                     FVSFHGNLDTPNPRDAQNIKAPVLVLHGADDPAVPQDQVDGFVAEMKATKADWQLVSY
                     GGAVHSFTNPEANVPGRNEYHPLVAARAFKAMDNLFDETFAK"
     gene            complement(15737..17641)
                     /gene="htpG"
                     /locus_tag="VFF22_05310"
     CDS             complement(15737..17641)
                     /gene="htpG"
                     /locus_tag="VFF22_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HZX16265.1"
                     /translation="MSVETQKETLGFQTEVKQLLHLMIHSLYSNKEIFLRELISNASD
                     ATDKLRFEALAKPELLEGGAELKIRVSFDKDAKTVTLEDNGIGMSREDAITHLGTIAK
                     SGTADFMQHLTGDQKKDSHLIGQFGVGFYSAFIVADKVDVFSRRAGSPASEGVHWSSK
                     GEGEFEVATVEKAERGTRIVLHLKAAEDEFADGWRLRNIIKKYSDHIALPIELPKEFH
                     GEEKDKPAEVEWETVNRASALWTRPRTEIKDEEYQEFYKHIGHDFENPLSWSHNKVEG
                     KLEYTSLLYTPARAPFDLYQREAPRGLKLYVQRVFVMDQAESFLPLYLRFIKGVVDSN
                     DLSLNVSREILQKDPIIDSMKSALTKRVLDMLEKLTKNEPEQYKSFWKNFGQVLKEGP
                     AEDFANKEKIAGLLRFASTRSEDGEQSVGLGEYIARMAEGQDKVYYLTGESYAQVKNS
                     PHLEVFRKKGIEVLLLTDRIDEWLMSYLTEFDGKHFVDVARGDLDLGKLDSEEDKKAQ
                     EEVAKAKEGLVERLKTALGEQVAEVRVSHRLTDSPAILAIGEKDLGLQMRQILEASGQ
                     KVPDSKPIFEFNPSHPLIEKLDGEADEDRFADLSHILFDQAALAAGDNLKDPAAYVQR
                     LNKLLVELSA"
     gene            complement(17741..18994)
                     /locus_tag="VFF22_05315"
     CDS             complement(17741..18994)
                     /locus_tag="VFF22_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX16266.1"
                     /translation="MDALLILGGLLLILAGLVWLVMRAFATGLLWGWASFIPPFTLLY
                     VLRHWRSARPAVILAGLGIIPLVVGLTLLANHDAQRLEAILSLKWLKPEVQAPAELAI
                     DLRGQLNGRPFAPQQAELLDGVLSLREGQDFFAQSELVIRLPKVDKGPVRVDVLPADE
                     GILPEVEISWLLPEQELPEARRLSRGYTLHLDLQPVPPNKLAGDFHLVLPPQFKTTLS
                     GKLELFRNGLRYQGDKVDRQFDSVDTVSHVIQDYLERRFATRRVELLPIGNLNFPLQR
                     VDLNVQARINGELQQLPILLQKQEKRGWQVAADSFPALPSAVAPPVAEAKPAAVAAVV
                     PERVSRPLDRRVRFSLDGLLRSPNRYENLSMRATTERGNSAEGRFHGIDQDGRIILRQ
                     RMSGQGEVSYSLLPEEITRIELLEP"
     gene            complement(19049..19495)
                     /locus_tag="VFF22_05320"
     CDS             complement(19049..19495)
                     /locus_tag="VFF22_05320"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZX16267.1"
                     /translation="MSTLSLNQLVREAHEQNNYDPLISLIPYAKLLGIECLRLGDDMV
                     FRLPANQDNIGNPTLPALHGGVIAGFMEHSAMLHLLMFMGIPHLPKIIDFSIDYLRAG
                     HYRDTFVQCQVWRQGRRVANVAITAWQTSQTEPIATARAHFKVDEP"
     gene            complement(19492..19956)
                     /locus_tag="VFF22_05325"
     CDS             complement(19492..19956)
                     /locus_tag="VFF22_05325"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZX16268.1"
                     /translation="MSTNPVLERAQRFLSVLRHCQVLGLQVQDATAEGLTLRLPYSPQ
                     IVGNPETGVIHGGAITTLMDTTCGISTVCVLPEFEICPTLDLRIDYMRPAEPNKDVFG
                     FAECYRVTENIIFTRGYAYQDNPDEPIAHVVGAFMRMGKPADMQRTAKGAGQ"
     gene            20107..20394
                     /locus_tag="VFF22_05330"
     CDS             20107..20394
                     /locus_tag="VFF22_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011534745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16269.1"
                     /translation="MQKTVAYAFMLAATLGLAACDKPSEDTREDAREAQSEAQESMND
                     AAEQSNEAANLEAEAARERAEENREEAEDANEPMPTAPITPTPAPTDQTNQ"
     gene            20534..20824
                     /locus_tag="VFF22_05335"
     CDS             20534..20824
                     /locus_tag="VFF22_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16270.1"
                     /translation="MQKPFTYALLLAAMLGLAACEKTPEDKLESAKESAAEAIESMGD
                     AAEETGEAIEQKADEVMGTEPTTGEKIEDAADDAADAISDAGDEVKDAVDGQ"
     gene            complement(20884..21276)
                     /locus_tag="VFF22_05340"
     CDS             complement(20884..21276)
                     /locus_tag="VFF22_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HZX16271.1"
                     /translation="MSIPFWCIFISALLIFIAKAPVAKAMAKEGDGHYDNHHPRAQQA
                     RLTGFGARALAAHLNSFEAFPLFAVGVLMAHVTNNHGILVDILAVTFVVARVLYLAFY
                     WADMHWQRSVVWVVGLLCSLLLMLTPAL"
     gene            complement(21273..22007)
                     /locus_tag="VFF22_05345"
     CDS             complement(21273..22007)
                     /locus_tag="VFF22_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 family protein"
                     /protein_id="HZX16272.1"
                     /translation="MPISDYYLPHLLAVLWFVVCWAGYTRYAQAKGRTTPCLASVLHL
                     YREDWMRRMLLRDNRIADANVIGNLERNASFFASSTLIILAGILTVLGASDRAVSLLA
                     DLPFVQAASRELSEVKLLCLGVVFVYAFFTFSWCMRQYNFAALLVGSAPMVGERHVTE
                     QERKAFAERTARVISMAANQFNQGLRAYYFGMATLSWFINPWFFMLVSAGVVLVLYRR
                     EFHSDVLEVMVYTQTPAFESPKEKSE"
     gene            complement(22108..23421)
                     /gene="brnQ"
                     /locus_tag="VFF22_05350"
     CDS             complement(22108..23421)
                     /gene="brnQ"
                     /locus_tag="VFF22_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HZX16273.1"
                     /translation="MTRLKGSDLLALGFMTFALFLGAGNIIFPPSAGMAAGENIGLAA
                     LGFLLTGVGLPLLTIVALARVGGGMALLTAPLGKRAGVLFAVAVYLAIGPLFATPRTA
                     VVSFEMGVAPFSGNSSTALLLYTLAYFAAVLFLSLNPGQLVDRIGKYITPVLLAALLV
                     LGGAALLIPAGEIGQVSPDYQSAPFVQGFLQGYLTMDTLGALVFGIVIATAIRDHGVT
                     DGKLITRYSVIAGLIAAVGLSLVYLALFYLGATSQGIAGEAQNGVQVLTAYVQHTFGT
                     AGSLLLAVVITLACLTTAVGLLTACGEFFSSLLPVSYRTVVIAFGLFSLLVANQGLTQ
                     LISFSIPVLVGLYPLAIVLVALSLLDDLWCSPARVFRPVMAVTLVFGLVDGIAAAGGK
                     ALIPSFFSQLPLADQSLGWLLPVLLTLVLAVVVDRVLGAPKTAAV"
     gene            complement(23719..24606)
                     /gene="sucD"
                     /locus_tag="VFF22_05355"
     CDS             complement(23719..24606)
                     /gene="sucD"
                     /locus_tag="VFF22_05355"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HZX16274.1"
                     /translation="MSVLINKDTKVICQGITGSQGSFHTQQAIEYGTKMVGGVTPGKG
                     GTEHLGLPVFNTVKDAVAATGATASVIYVPAPFCKDSILEAAFGGIKLIVCITEGIPT
                     LDMLDAKVKCDELGVVLIGPNCPGVITPGECKIGIMPGHIHLPGKVGIVSRSGTLTYE
                     AVKQTTDAGFGQSTCVGIGGDPIPGSNFIDILKLFQEDPKTEAIVMIGEIGGSAEEEA
                     AAYIKANVTKPVVSYIAGVTAPAGKRMGHAGAIISGGKGTADEKFAALQDAGVKTVRS
                     LADIGKALAELTGWEVKKA"
     gene            complement(24606..25772)
                     /gene="sucC"
                     /locus_tag="VFF22_05360"
     CDS             complement(24606..25772)
                     /gene="sucC"
                     /locus_tag="VFF22_05360"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HZX16275.1"
                     /translation="MNLHEYQGKQLFAEYGLPVSKGFAVDTPEDAAAACDKIGGTEWV
                     VKAQVHAGGRGKAGGVKLVKSKEDAKAFAANWLGKRLVTYQTDANGQPVTKILVESCT
                     DIAKELYLGAVVDRSSRRIVFMASTEGGVDIEKIAHETPEKILKATIDPLVGAQPFQG
                     RELAFQLGLEGKQVAQFAKIFVGLAKLFQDHDLALLEVNPLVIKADGDLHCLDAKINI
                     DANAMYRQPKLKTFHDPSQDDPREAHAAKFELNYVALEGNIGCMVNGAGLAMGTMDIV
                     NLHGGKPANFLDVGGGATKERVTEAFKIILSDSNVAAVLVNIFGGIVRCDMIAEGIIG
                     AVKEVGVKIPVVVRLEGNNAELGAKVLAESGLNIIAATSLTDAAQQVVKAAEGK"
BASE COUNT         5486 a         8046 c         7401 g         4915 t
ORIGIN      
        1 tcaagcggca aacggattgc atcagccaca gggcttcctc ctttgttgtt tttgtggagc
       61 caaccaagca attgcttggt ttgactattc cacgcacaaa ggcggcccgc aagaggagaa
      121 atgtttcggt gtgtagtgag gtgtcaggaa cggcttggat gcttatgggc agacaattcc
      181 atcaacccag cggccagctc atcaccttcc agcaacgcca gactggcggt gttcatcggc
      241 agcggttgct gacgatgacc atgtatcagc aggcccgcca aggcgccaac cagcggctga
      301 tgggtcacca acaacagctc atcgaacgca taaccgtcca gttcccgcag cgcttggcgc
      361 agatcactct ccggcgttag ccagggcacg gtctgccgga taccgctaaa ggccaggacg
      421 tcgcaggcca gatcggccgt ctgctgcgcc cgcacatagg ggctgaccag tacggcctgc
      481 aacggccgcc ctaacaactg tgcagccgcc tcacgcacct gctgccgacc ataggcagtc
      541 aggttgcgtt cggcatcagt gcgcgcagtc ggttcggctt caccgtgtcg cagtagccag
      601 agcttcacag gcttacccca ataagccgac gactcaaagc ttcggctctt cgtcacgcac
      661 cggatgggca gccggcggaa tgctgtgcgg cgtttcacct tgtggcgctt gcggggttgg
      721 ccaatcggcg aacggccagg gcttttcatc gctattaaaa gtgccaaagc ggccgatctg
      781 cgccaggtat tggctcaggc tgtcaccgaa gcccagcagg ccagcattcg gagcaccgta
      841 gaacagacgg taacccagct gcagcagcac cacggcgccc agaaggattt ccgccagttg
      901 ccagacgatg gtgaagatca ccatccagag gatgcgcagc agaatcgatt cgcgctgcag
      961 ctcctgattg tcattactca tgctcatctc cttgcagggt ctagaaaggt aaaactcaga
     1021 aaccggtggt ggggataaag tcgacgtcgg tcttcggttc agcattcatt agcgcaccga
     1081 tcacctgatc cagcgtgcgc ccttcaaaca gaatcgcatg caagccagtt accagcggca
     1141 tatagacctg cagctcctcg gctttggctt ttaacacttt aatggtgttt accccttctg
     1201 ccacttcgcc caaacgctca accgcatcct ccaggctcaa gccttcaccg agggcgaaac
     1261 cgacctggta gttgcggctt ttcgccgaag agcaggtaac gatcagatca cccaccccgg
     1321 ccagaccgag gaaggtcata ggattggcgc ccagtttgac cgcaaaacgg gtcatttccg
     1381 ccagcgcgcg ggtaatcagc atgctcttgg tgttttcgcc catgcccatg gctgctgcca
     1441 taccagcgat gatcgcgtag acgttcttca gcgccccgcc cagctcgacg ccaaaacggt
     1501 cgccactggc atacacgcgg aaggtgcgcc cgtgcagtgc ggcctgcacc tgcaagcaga
     1561 ggtcttcatc ctcgctggcg acgaccgacg cggtcagcgc atgctcggcc acttcacgcg
     1621 ccaggttcgg ccaagagagc acgccgatac gcgcctgcgg agcgatatct tcaagaatct
     1681 ggctcatcag caaaaagctc tgcgcctcga tgcccttggt ggtgctgacc aacaacttgc
     1741 cgctcagcaa ctcagccacc ggctgcaacg cctggcgcag cgcgctggac ggcaaggcaa
     1801 caaaaacgag ctggcaagcg ttcagggtcg cggccagatc ggttaccggg tcaacgcctg
     1861 gatgaatctt gatgcctttg aggtaacgcg gattctcacg ggtcagccga atcgcctggg
     1921 cctgttgtgg gtcgcgcatc cagtgcagaa cctgttggcc attttcggcc agcaaattag
     1981 caatcgcagt gccgaagcta ccgccgccga gcactgcgat gggttgctga tcagtcataa
     2041 cgtatccgtg gtaaaccatt gaaagtggcg aatcgctcgc attatacggc gacacccgta
     2101 cgcagccagc ttttagcagg cccgcaagcg ctggcaaagc caggcagctc agttaacatg
     2161 cagtctttca aacgcaaaca aggttgtggc gtgatcgata gcacccaagc ccggcttcac
     2221 cctgagaccg cgaaaaaccg gccagccctc cagcgcacgc acgttctgcg cagtagcgga
     2281 gtggcccgat gatcctgcgc cgtggctgga atatccatac ccgcacgcaa ttgatcagtg
     2341 tcggccccgc cctgctgctg accctgctgc tcaccggctt cttcaccttt gcccgcctgc
     2401 aggacctgcg tcaggagctc aaccacaacg gccagctgat cgccagccag ttggcgccag
     2461 ccagcaccta cggggtgatt tccggcaacc tcgatgtcct cgacgcccta cttcaagcaa
     2521 ccttgaagac cccgcatgtg cgctttcttg aagtacgcga ccacaccggc aagattctcg
     2581 tctacgtcga acagacgccc ggcagcggcc acgccagcag ccaggtcgag gtgttccagg
     2641 cgccgatcca tctgcaacgt gcagcgctga acaactccac tcaacagacg ctgaccgatg
     2701 actacctggg ttatgtcgtc gtgggcatgt ccaacgatgc cttcaacacc cgccagcagg
     2761 aaattctgtt caaggccacc ctgctcgccc tgttcgccct ggtcctgacc tttattctcg
     2821 cccgtcgcct ggcccgtcac ttgtcgcagc cgcttagcgc catgggtgaa gccgtcagcg
     2881 cgatccaggc cggcaactac cagaccaccc tgcccgaagt cggtgaaggc gaattacagg
     2941 cgctggcgcg acatatcaac aacctcgccc acggcctggc cagcgccagt gaagaacagc
     3001 agcgggccat ggcactgctg attcaggccc gcgaacagtc agagttggcc aaccgcgcca
     3061 aatccgactt tctggccatg atgagccacg agttgcgcac cccgatgaat ggcgtgctgg
     3121 gcatgctgca gctactggaa accacggaaa tgacccagga gcaggcggaa tatgcggcgc
     3181 tggccaccga atccaccgag catctgttga aagtcatcaa cgatattctc gacttctcgc
     3241 gcatcgagcg cggcgcgctg gaactggagc gcatcccttt cagcctgctc gaactgctgc
     3301 aaagctcgat tcaagtgttc cagcacagcg cccagcagcg cggcctgcaa ttgctcttgg
     3361 aaacccagag cggcctggag cagttgcagg tccagggcga cccgacgcga atccggcaga
     3421 ttctggtcaa cctgatcggc aatgccctga agttcaccga aaccggcagc atccgcgtac
     3481 acagcacctg gcaggcgctg gacaatcagg tgctgtggtt cagttgctcg gtacatgaca
     3541 gcggtatcgg aatttctccc gagcggctgg aacatatgtt cgatgctttc cagcaggccg
     3601 acacttctat ttcaagacgt tacggcggca ccgggctggg cttgcccatt gctcgcaccc
     3661 tcgccgaacg catgggcggc accttgcgcg cagtcagcca ggagggcgag ggctcggtat
     3721 ttaccctgga agtgccactg ccgttttccc agcatgttgc gccagctgtc agcgacacct
     3781 cgctacctgc tgatcagggt aacggccagc cggtgctgct ggtggaagac aacccagtca
     3841 accagaccgt tatcgaggcc atgctgcgca gcctgggcta tcaagtcagt ctggtcggcg
     3901 atggcgccca ggccgtgcat caggtcagcc agcagcatta cgccgcgatt ctgatggatt
     3961 gccgcctgcc gatcatggat ggctatgaag ccacgcggca gatccgccgc ctgcccggtt
     4021 gcgccgaatt accgattatc gccctgaccg ccaatgccct gcagggcgac cgcgaggcct
     4081 gcctgcaatc gggcatgaat gactacctgg caaagccctt caaacgggca gatttgcagc
     4141 gaatactgca gcgctggctg ccacctcgcc cttcgacggg tagtcaagaa ggctcaactg
     4201 ctgcaggcta gagccatagg tctatgacag accaacaaga gcggagtaca actgtactca
     4261 caaggctgtg actttcacta caacgcaaca gtctatgact aggctgttgg caggcgcagc
     4321 agactgcgcc gccagccagg acgattcgcc cagccttggc gcatgggaca tattgaggag
     4381 ctcgcatgac caaacaaaac gccttcaccc gggaagaact gctcgcttgt ggtcgcggcg
     4441 aactattcgg cccaggtaat gcgcaactgc ccgcgccgaa catgctgatg atcgatcgca
     4501 tcacccacat cagcgacacc ggcggcaagt acggcaaggg cgaaatagtc gccgagctcg
     4561 atatcactcc tgacctgtgg ttcttcgcct gccatttcga aggcgacccg gtgatgccag
     4621 gctgcctggg cctcgatgcc atgtggcaac tggtaggttt ccacctcggc tggcagggca
     4681 accccggccg cggccgcgcc cttggttcgg gcgaagtgaa gttcttcggc caggtgctgc
     4741 cgaccgccaa gaaagtcact tacaacatcc atatcaaacg caccatcgcc cgctccctgg
     4801 tcctggctat tgccgatggc accgtcagcg ttgatggccg cgaaatctac agtgccgagg
     4861 gcctgcgggt tggcctgttc acttctaccg acaatttcta aaggactcat cgcatgcgtc
     4921 gcgtcgtgat taccggcctg ggcatcgttt cctgcctggg caatgacaaa gccaccgtct
     4981 ccgccaacct gcgtgctggc aagtcaggca tccgcttcaa ccctgcctac gccgaaatgg
     5041 gcctgcgcag ccaggtgtct ggctcggttg acctcaacct ggaagagctg atcgaccgca
     5101 aggtctatcg ctttatgggt gacgccgcag ccttcgctta cctctcgatg gagcaggcga
     5161 tcaaggattc gggcctcagc gcggaagaaa tctccaaccc acgcgtgggt ttgatcgcag
     5221 gctctggcgg tgcatcgacc ttcaaccaga tggaggccat ggacatcctg cgcgagaaag
     5281 gcgtcaagcg cgttggtcca taccgcgtac cgcgcaccat gggtagcact gtttccgcct
     5341 gcctggctac gccgttcaag atcaagggcg tgaactactc gatctcctcg gcctgcgcca
     5401 ccagtgccca ttgcatcggc aatgccatgg aacaaatcca gctgggcaag caggacgtag
     5461 tgtttgccgg cggcggcgaa gaagaacatt ggagccagag cttcctgttc gacgccatgg
     5521 gcgccctctc cacccagtac aacgaaaccc cggagaaagc ctcgcgtgcc tacgacgcca
     5581 agcgtgacgg tttcgttatc gccggcggtg gcggcatggt ggtagtcgaa gagctggaac
     5641 acgccctcaa gcgtggcgcc aagatctatg cggagatcgt cggttacggc gcgacctccg
     5701 atggctacga tatggtggcc ccgagcggtg aaggcgcagt gcgctgcatg cagcaagccc
     5761 tggccaccgt cgatacgcca atcgactacc tgaacaccca cggcacctcg actccggtcg
     5821 gcgacgtcgc ggaaagccgc gcagtacgcg aagtattcgg cgccaaggcg ccagccatca
     5881 gctcgaccaa gagcctgtcc ggtcactccc taggcgccgc cggcgtacag gaagcgatct
     5941 actgcatgct gatgatggaa ggcaacttta ttgccggctc ggtcaacatc gacgaagtcg
     6001 acccggaaat cgccgacctg ccgatccagc gcagcaccgt cgagaacgcc aagctcgaca
     6061 cagtgatgag caacagcttc ggcttcggtg gcaccaacgc caccctggta atgaagcgtt
     6121 gggctggcaa ataagtccca accgttcgct tgccgcatag aaaaggccac ccctcggggt
     6181 ggcctttttg ttgtgcttga cgcagtactc actcaaatag agaagcgcgc caccagtgca
     6241 ttcaggtcca ctgccaaacg cgacaactcc tgactggccg cactggtctg gttggccccc
     6301 gccgaagact gcatcgacag atcacggata ttcaccaggt tgcgatcgac ttcgcgagcc
     6361 acttgggcct gttcttccga cgcgctggcg attactaggt tgcgttcgtt gatctgcgaa
     6421 atagcagcag caatctcatc caacgccaac ccggccgcct tggccacatc cagcgtcgaa
     6481 cgcgcccggc tgttgctgtt ctgcatggcg ttaaccgctt tgtcggtgcc ctgctgaatg
     6541 ccgctgacca tttgctcaat ctcacgggtc gactgctggg tacgatgcgc cagggcgcgt
     6601 acctcatcgg ccaccacggc aaaaccgcgc ccggcttcac cggctcgcgc agcttcaatc
     6661 gccgcgttca gcgcgagcag gttggtttgc tcggcaatcg agcggatcac atccagcacc
     6721 ttactgatat cgcgcacctg gcccgccagt tgctcaactt cgctggcggt attagtcacg
     6781 tcatcagcca ggtggctgat cgagtcaaca gtctggcgta cctgctcgcg gccatgctgg
     6841 gctgtacggt tagattcact ggaggcctcg gaagtgctaa cggcattacg cgccacctct
     6901 tccacagcgc tggtcatctg gttgaccgcc gtagccgcct gctcaatttc gttgttctgc
     6961 tggtgcaacc ctcgggtgga atcctcggtc accgcactga gctcttcaga cgccgaggcc
     7021 agttgcgtgg aggaatcaga aatattctgg atagtgctgc gtaggctctg ctgcatgctt
     7081 ttcaaggcgc ccagtaaacg cgccggctca tcactgccct gcacctcgat tgactgggtc
     7141 aggttgccgc tcgccaccac ttcggccacc cgcaccgcct cggtaagcgg cgcgacaatg
     7201 ctgcgcgtta gcagcagcgc cagaatcacc gttaggccaa gcgccagggc aatcatcaca
     7261 atcacccaca cgcgcgagct gacaaacacg gcctcggcac tgtcagccgc tgcagtggca
     7321 ccgacgcggt ttaattcaga aagctcgacc agcgccttgg ccatgctatc ggcgtgaccg
     7381 ttaagcgggc cgttggcgac tgccagcgcc gtatccagat cgctctgacg cacggcctca
     7441 acgatcttgc tctgttcctg cagataactg ctttccgcct gtttaaagcg ctggtaaatc
     7501 agcctttccc cctcactggc aatcagcttt tcatagccgt cttgggcctt gttcagcacg
     7561 cctctgagtt cttcaaggcg ggccaggttc tgcctagtca tgccctcatc ggtattgagc
     7621 atcaagcgca aggtcagcgc gcgtaggcgc aggatgtcct ggttcatctc gcctagattg
     7681 agaatgctgg gcaaccagtt ctcgttgacc tgctgcgact gcttgtgcat ttctgccatc
     7741 tgcagcagcg agaagctgcc gagtataaga accagcaacg ccaccaggcc aaagccaaga
     7801 gacgccctgg gcgcaatatt gaggtttcta agggtcattg ggagcaatcc tttacggtct
     7861 taagcattaa agcgatgcgc tggctatcag gcttttgctt tcctgcgtac ctcggccagc
     7921 acctacaggt acaactatcg actcccaaac acgcttcttg accttttaat cataaaaaaa
     7981 tccgagcaat ggctcgggtt acggtaacga cttgggataa ctcagcgcac ggtaaaccgt
     8041 tgctccagca atttgcgatc atcctgaaac accataaagt gccactcacc tggcaccact
     8101 tcgtgctttt cggtgaattc aaaagccatg acatcctgcg gcgcgccggg caccagcttt
     8161 tgtatcacta cgaatttgtc atgccgcgtg ccctgtggag tgaccactcc cggggtgagg
     8221 tacagcaacg tcagcggcga gtcatcggcc atcttgcctg ccaggctgta gcgcagaccg
     8281 aatttggtac cgagcttggc gggaatcacc tcgccctgtt cgatttgctg gttactgcgg
     8341 gtcaacaccc gctcaccttc ctgaaagtcc tgataatcgc tggcgaaaac gccgtactcg
     8401 accgggcctt ctacccggac ttcggcctgc gccaggctgc tggtcaatac aagtgcggcc
     8461 agggcactaa aacgggtgta ttgcatgtcg cgctcctttt ttagatggcg cgagcctatg
     8521 acgcccgtgt gacagggcta tgacagccct actccgccag ctcggcctgc acccggcgtg
     8581 gcagcaccgc aagaaagtta tcgcgcgcca cctgatgggc aacatcctct ggcaaggcgt
     8641 cgagaaacgg tttaaagccg ctcatatagt cagccaggct ggtgaagcgt ccgaccacat
     8701 cggagccgag cataaagcgc gccgggtaac tgctgaccaa tttcacccag gccggatctg
     8761 gcttgccgtc ggcatccagc aggtacggct ggagcacggt ccaggacaga tcgatataca
     8821 gattggggta ctgcttgagc atccgctcaa gcgtgggcag gaggaaatcc agcttctcct
     8881 gatgccgatg aatttccatg ctggtgccgg catgcgccca gataaaccgt acatgcggat
     8941 ggttacgcag cgggtcctcg atctcctgca ggtacagcgg attgcgctcg cgcttggagg
     9001 tgatattgga atgcagcatc accggcaggt cgtattcggc ggccaagtga aagacccgcg
     9061 ccagggcctc gttgttggcc cgcggcgcgt cgccatggat cagcgcggtg aggtcgtcat
     9121 gccgggtaaa aatctcgccg atgccttgcc acagaccagg gtcgagttcg agcatacgac
     9181 ggatatgtgc atcggcattc ttgtcgttcg gattaaagcc cgacagaaac gggtgcagac
     9241 gtttgcgctg ttcagcgggc acctgtttgt aggcatgcgc caccaacaca tcggtggcgc
     9301 tgtaccaata ggcattggca tcgtcacccg cgtaataacg agggcgcttg ggctcatcct
     9361 catgccattt cttcgccacc ggaatgcctg agagcatcac atgctcgata ttgccggccg
     9421 ccatctgctc gaataactcc gtcatacccg cgctttcttg aaagaaatcg acgtaatgca
     9481 gatgcgcatc gctgtagcga tagtcgcggg cctgggccat tgaactcagc aggccgagta
     9541 acagggcaat actcatacgc gatacaggca agggcatgtc ctcctgacag tcagagcagc
     9601 atagacccca ggcggcaggc gcaagttcag gcgcagagcc gcgactggcg agcaaacagc
     9661 gccacaaagg cccaaaccac agcatgcagc accagaacag ggcggccata gcgactgttt
     9721 tccagcgtca gcagcatgac gcggcacgtc agtgatggca ctttgcttgc agtacaaccg
     9781 ccagacccta gaaggaattc accatgcttc agctattgcg ctcgacccca cgcaagaaca
     9841 cggacgacat tgcgctcgat gcgctgttca atcagcacga cctcaccgcc cgtctgagcg
     9901 atagctatcg ctggatgtcg cgcctgaatg acttcagcgc aggcctgcac caacgcatcc
     9961 gtaccagcct gggcgcagca gttggtattg cttcgcatgc cccacaactg gcacgtattg
    10021 ccgccgccaa ccagcaaagc gggcaaactc tggcacagtc atcggaactg atcgccagcg
    10081 ccagcgaaca ggtcaccacc accctcgacg ccgagttggt gcccggcgcc gccgaagtcg
    10141 cacagttgtc cgccgaggtc accgccaccc tgcatgattg ccagcaaagc ggccaggcag
    10201 tgctgcaaca ggtcgaggtg atcggcaaaa gcgaacagca gctggagctg gtgattcagc
    10261 aactgaccag ccagcttgaa gaggtcagca aagtgatcgg ggtaatcgcc agcatttccc
    10321 agcagaccaa cctactcgcg ctgaatgcgg ccatcgaggc tgcccgcgcg ggcgagcatg
    10381 gccgtggctt tgccgtggta gccgaggaag tgcggcgcct ggccgggcat accaccgacg
    10441 ccaccactca ggtcagtgac atcatcgagc gttttcgcag cggcatgcag caactcaccg
    10501 aagcaggcag ccacatgcac caggccgtgg ccgatggccg cgatggcatg ctcgccgtgg
    10561 gcggtggtct ggacagcacc cgccaggcca tggatcgcct ggacaggcgc gtgggccaga
    10621 tcgccgctgg caccgaacag atcggccaag ccgtgcgctc aatcaacggt gacgtgcaga
    10681 acatcgccag ggtcgccgga gaattgctcg gcaaggccag ccaggtgctg caccacagcc
    10741 aggctgtgcg cgaagatggc gaccgcctgc tggcggggct gggtgatttt cgcctggaga
    10801 tccaccacgc cgtgcaggcc agtgtcgaac agctggcgcg ccaacccggc atgagcgcgg
    10861 atgtggccac cgccgaacgc ctgcttggcg acaccctcaa gcgagatgcg cggttcgagc
    10921 tgttctacct ggtcgacagc cgcggcgtgc agctgtccga aaacatcttt gccaccgatg
    10981 tgaaccacag cgaaagcagc agctgcaaag gccgcaattg gcagcaacgt ccgtggttcc
    11041 gcgccgctgc tgatagcctg tgcagccata tcacccaggt ctatcgatca tcggcaaccg
    11101 acgacttctg tttcaccgtc tcggtgccaa tcctcgatgc ccgtggccaa ttgctgcgcg
    11161 tactgggggc cgatgtgcgg ctgtctgcac tggtctgagg cccgcgccat gagctaggct
    11221 gaccactcac tcagcccatg gcgtcggagc tcacatgatc agcatcagca acgagaaagg
    11281 tctactgcag cgcatcagca tcggcccgca caccctgcac agcgatgtct cggtggagct
    11341 cggcggtgaa ggcgcaggcc ccgaaccgca tgacctgttc gacgccgctt tgggcgcgtg
    11401 caaggcactg accctggccc tgtatgccaa gcaacgtggt ttgccgctgg aaggcctcga
    11461 cgtgaagctc aaccgcgatg acagccagga gcgccaaggc atctaccgtc tggatgtcga
    11521 actgaccctg cacggcgcac tggatgacgc gcaacgccag caactgctgc gcgtcgccga
    11581 taaatgcccg attcacaaac tgatgaccag cagcgaggtt caggtcagta ctcagctgag
    11641 cgagggcgcg taatgaacga ttttctgctg atccaaccgc gcccggaaaa cctcggcggg
    11701 caacctatcc tgcgccccct gccctcagcc aaatgccgca gcgtcgggcc gttcgtgttc
    11761 ttcgaccata ttctggaaaa cacctacccg gccggcagcg gcatgaatat caaccagcac
    11821 ccgcatatcg gcctgtccac cctcacctac ctgttcaaag ggcaactgca acacaaagac
    11881 agcctcggct cgaatcaact ggtcagcccc ggcgaagtca gctggatgac cgcaggccgt
    11941 gccgtggcgc atgtcgagcg caccccgcag actctgtggg aaagcggctc gcacatgcat
    12001 ggcctgcagg tctggctggc catgcccacc gctgaggaac aaggcgagcc cagctacagc
    12061 caccaccccg cagacagcct gccgcgcagc gccagcatgg gcgtggacat ctgcctgatt
    12121 gccggcactg gcttttgcct gcaatcacca gtgccggtgc tgtcgcccac tatctatgcc
    12181 gagatcaagc tggccgcagg cagcgcgctg cagatacccg ccgaacatgc cgaacgcgcg
    12241 ctgtacctga ttgagggcga ggcgacactg gacgaccagc aactgtcgct gcgtggcttg
    12301 ctggtgctgc ccgaaggcga gacttacacc ctcagcgctt gcagcgagag ccacctggtg
    12361 ttgatcggcg gtgcaccgat tggcccgcga cggatcaact ggaacttcgt cgccagtgac
    12421 ccagcgctga ttgatcaagc ccgccaacgc tgggccgcag aggactggcc aacagtgctg
    12481 ggagaaaccg agcgaatcga gttgccgcgc tgacggagaa gatttgttgc actcccgaga
    12541 catccaaagc gcaggtgctc gggtataacc cttggcacgc tcacactctc aggaaccctg
    12601 tgcatggcgc agccgctgtt tgattacgaa agccatctgg aagcctgcgc ccggcaggac
    12661 gaccgcgcat tccaggcgct ctaccaacag gaggccgcac aaatgctcgg gttggccatc
    12721 accctgctcg gccgccgtga tcaagccgaa gactgcgtgc acgatgcctt tgtgcagatc
    12781 tggcgcaacg cccagcgttt tcagcgcgcg cttggcagcg gccgtgcgtg gatctacagc
    12841 atcctgcgct accgcgcgct gaatcagctg cgcagccgtg gccgcacggt ggagctggat
    12901 gacgacttta ttgaacgcgc cgccgacgac agcccgcagg ccgccgaact gctggaacaa
    12961 caaggcgaac agcagcaact gcgccgttgc ctgcaaagta tcgaacgcaa ccgccgccac
    13021 ccgatcctac tggccttcta ccgtgggctg acccatgaac agatcgccaa ccgcctgacc
    13081 accccgctgg gcaccatcaa gggccgtatc cgcgccggtc tgcgcgcact gcaggagtgt
    13141 ttgcaagcat gattccgcag caacctgccg aacgccgagc attgatcggc gaatacgtcc
    13201 tgggcctgct tgaagaggcc gaagctgctg aagtgcgcga gttgatcgag caggatgccg
    13261 aagccgcgca aatggccctg caatggcaac agcactttct cgaactgagc gatcagttac
    13321 cgccgcaaac accctcggcg cagttatggc aacgcctgca ggacagcctc cgcctgacgc
    13381 cagccgtaag ccgggtaaac agctggctga cctggtggca aagcctgacc acctggcgcc
    13441 tcaccagtgc cgccctggca ttcgccctga tcgtcgcgct aatgcctaac ctgtggcgca
    13501 ctgacatggc tggcaatagc tacaccgtgg tgctgcaagc cccgggggaa gcggccaaac
    13561 caggctgggt ggtgcatgtg gatggcagtg gcagcctgcg cctgcaaccg ctgctggaag
    13621 atcaaatccc ggccgaccgt tcggtgcaat tctggacgtt gatcgaccca gccatcggcc
    13681 cgcgctcttt aggcctggtc gaaccaggta agtcgctgca actgtctgcc gaacagattg
    13741 gcgcggtaca agccggccaa ctgttcgagc tgaccctgga accggcaggc ggctcaccta
    13801 aggatcggcc aaccggcaag gtgctgtata tcggccgtgc ggttatggca tcggttaact
    13861 gaggtcgttt gcaaaaaaac tgaatttatt ttcagtcggc gacatccaga cgttctctcg
    13921 tgggtatagc tatcaggtgt ttcatcgagg cacctgcaag gcgggccttc gccggccttg
    13981 catcgatgct ggataaccac agatgagaga acgcatatga acagcctcaa taccctgctg
    14041 accgctgccg ttgccactgc caccctcagc cttgccgccg gcactgccag cgcggccacg
    14101 ctgctggcac tgggtgccga cggtcagctg ttcaagatcg acagcgccag ccagaaaatc
    14161 accgcaagca tgcccgtcag cagcccaagc ccgctgcgcg gcatcgatgt gcgcccggcc
    14221 aacggcatgc tctatgcgct aggcagcgac aatcagctct acaccctgga cctggccact
    14281 ggcgcagcca gcaaggtcgc cagcctgaac aagcagctgc cgggcagcgg ccaggcggtc
    14341 atcgacttca acccggtggc tgacaaattg cgcatgctgg ctgccgatgg caccagtttt
    14401 cgcgtcaatg ttgaaactgg tgaagtggtc gtagacggtt cggtggccta cgccgcagag
    14461 agcccctacg ccggtaaaag cgctcaagtg gtagcgggtg cctataacaa cgcctatgcc
    14521 ggcacccagg ccaccgcgct gtacaacgtc gacctagcca gcagcagcct gatgctgcaa
    14581 aacccgccaa acgatggcat tcagcaagct gtgggcaaga tcgttgatgg tctgcagaac
    14641 gcggccctgg atatcgccag cgacggcaaa ggcggcaaca ccgcctacct gctgactggc
    14701 accacgctgc ataccctgga cctagctact ggtaagccga ccaccctggg cgaaatcgcc
    14761 gacctgccgg gcaatatcat tgatattgcc gtgctgccca acatgtaagt ccggatgcgg
    14821 gagggccatc cggccttccc gttcacggtt tatttggcaa aggtttcatc gaacagatta
    14881 tccatggcct tgaacgcacg agctgccacc agcgggtggt actcattgcg ccccggcaca
    14941 ttagcctctg ggttggtgaa ggaatgcacg gcaccgccat agctgaccag ttgccaatcg
    15001 gctttcgtcg ctttcatttc cgcgacaaaa ccatcgacct ggtcctgcgg caccgctgga
    15061 tcatccgcgc catgcagcac aaggaccggc gccttgatgt tttgtgcatc acgcggatta
    15121 ggcgtatcga ggttgccatg gaatgacaca aatcctttca gatcggcacc cgaacgggcc
    15181 agttccagta cagccccacc accaaagcag aaaccgatag cgcctagccg cgcggtatcc
    15241 aatgccacct gcccggcctg cgctttgagc acctcaacag cctcctgcgc ccgcttgcgc
    15301 atcaacggtc tatcgctgcg cacagtcgtg gccgctacct tagcttcatc ggcattagtc
    15361 gggcgaatag ctttgccgta taggtcggca acgaatatga cgtatttatc agcagccagc
    15421 agcgcggcct tggttgccgc gttttcatta acgcccatcc aattaggcac catcagcagg
    15481 cccggacgcg gcgtagtgac cgcatcgtca tagaccacca agccttcgaa ggcttccccg
    15541 tcgatttcat aaggcaccgg cttgaccacc acggcagctt cggcgacacc gaccagaccg
    15601 gccagcgcca agggtaaaag tgattttttc atcgacccga atccctcttg aataagtagt
    15661 gctcaacagt agcctctgtg tgcaccgcgc caagaaaaaa gcccgctttc gcgggcttaa
    15721 ttctttacgg cacgtgtcag gcagacagct cgaccagcag cttgttcaag cgctgcacat
    15781 aggcggccgg gtctttcagg ttgtcgcctg cggccagggc agcctggtcg aagaggatat
    15841 ggctgagatc ggcaaaacga tcttcgtcag cctcgccatc gagcttctcg atcagcgggt
    15901 ggctcgggtt gaattcgaag atcggcttgg agtccggcac cttctgcccg ctggcttcga
    15961 ggatctggcg catttgcagg cccaggtcct tctcgccaat ggccagaatc gccggcgagt
    16021 cggtcaggcg atgcgataca cgcacctcgg caacctgctc acccagcgca gttttcagac
    16081 gctcgaccag gccttctttg gccttggcga cttcttcctg ggcctttttg tcttcttccg
    16141 aatccagctt gccgaggtcc agatcaccac gggccacgtc gacaaaatgc ttgccgtcga
    16201 actcagtcag gtagctcatc agccactcat cgatacggtc ggtgagcagc agcacttcga
    16261 tgcctttctt gcggaacact tccaggtgcg ggctgttctt cacctgggcg taggactcgc
    16321 cggtgaggta gtagaccttg tcctgacctt cggccatgcg cgcgatgtac tcgcccagac
    16381 caacgctctg ctcgccatct tcgctgcggg tagaggcaaa gcgcagcaga ccggcgatct
    16441 tctccttgtt ggcgaagtct tcagccgggc cttccttgag tacctggcca aagttcttcc
    16501 agaagctctt gtattgctcg ggctcgttct tcgtcagttt ttccagcata tccagcacgc
    16561 gcttggtcag cgccgacttc atcgagtcga tgatcggatc tttctgcagg atctcgcggg
    16621 agacgttcag cgacaggtcg ttggagtcga ccacaccctt gataaagcgc aggtacagcg
    16681 gcaggaacga ctcggcctga tccatcacga acacgcgctg cacatagagt ttcaggccac
    16741 gcggcgcttc acgctgatac aggtcgaacg gcgcacgcgc cggggtgtac agcagcgagg
    16801 tgtattccag cttgccttcg accttgttgt ggctccagct cagcgggttc tcgaaatcgt
    16861 ggccgatgtg tttgtagaac tcctggtatt cctcgtcctt gatctcggta cgcggacggg
    16921 tccacagagc gctggcgcgg ttgacggttt cccactcaac ctcggcaggt ttgtctttct
    16981 cttcgccgtg gaactccttc ggcagctcga tcggcaaggc gatatggtcg gagtatttct
    17041 tgatgatatt gcgcagacgc cagccatcgg cgaactcgtc ttcagccgct ttcagatgca
    17101 gcacgatacg ggtaccgcgc tcggcctttt caacggtggc gacttcaaac tcgccctcgc
    17161 ctttgctcga ccagtgcacg ccttcgctgg ccgggctacc ggcgcggcgg ctgaacacgt
    17221 caaccttgtc ggcgacgata aaggccgaat agaagcccac accgaactgg ccgatcaggt
    17281 gcgagtcctt cttctggtcg ccggtcaggt gctgcataaa gtctgcggtg ccggactttg
    17341 cgatggtgcc caggtgggta atcgcgtctt cacggctcat gccgatgccg ttgtcttcga
    17401 gggtgacggt cttggcgtcc ttgtcgaagc tgacgcggat tttcagctca gcgccgcctt
    17461 cgagcagctc aggcttggcc agcgcctcga aacgcagttt gtcggtggca tccgatgcgt
    17521 tggaaatcag ctcgcgaagg aagatttcct tgttcgaata cagcgaatgg atcatcagat
    17581 gaagcagttg cttcacctcg gtctggaagc ccagggtttc tttttgagtt tccacactca
    17641 tgctctctta ctccacttgg ttgacataag ccgcagtgcg acggatgtcc tacaggtggg
    17701 ggcatgttgt gggatttcaa gtgcttggct gcagcttgcc tcagggctca agcaactcga
    17761 tgcgagtaat ttcttccggc agcaggctgt agctgacttc gccctgcccg ctcatgcgct
    17821 gccgcaggat gatccgcccg tcctggtcga taccatggaa gcgaccctcc gcgctgttac
    17881 cgcgctcggt ggtcgcgcgc atgctgaggt tttcatagcg gttggggctg cgcagcaagc
    17941 catcaaggct gaagcgcacg cgccggtcca gaggccggct gactcgctcc ggaacaaccg
    18001 ccgctactgc agccggtttg gcttcggcaa cgggcggtgc aactgcgcta ggcaatgctg
    18061 gaaaactatc ggcagcgacc tgccagccac gtttttcctg cttctgcagc aggatcggca
    18121 actgctgcag ttcaccgtta atccgtgcct gaacgttcag gtcaactctc tgcagaggga
    18181 agttcaggtt gccaattggc agcaactcaa cccggcgagt ggcaaaacgg cgctccaagt
    18241 agtcctgaat cacgtggctg acggtatcga ccgagtcgaa ctgacgatcg actttatcgc
    18301 cctgataacg caggccattg cggaacagct caagcttacc gctcagggtg gtcttgaatt
    18361 gcggcggcag caccaggtgg aaatcacctg ccagcttgtt cggcggcacc ggttgcagat
    18421 ccagatgcag ggtatagcca cggctcaagc gccgcgcttc gggcagctcc tgctccggca
    18481 ataaccagct gatttccact tccggcaata tgccctcgtc ggcgggcagc acatcaaccc
    18541 gcaccgggcc cttgtcgact ttaggcaggc gaatcaccag ctcgctttgg gcgaagaaat
    18601 cctggccctc acgcaagctc agtacgccat cgagcagttc ggcctgctgc ggcgcgaacg
    18661 gtcggccatt caactgaccg cgcaaatcaa tagccagctc ggccggtgcc tgtacttcgg
    18721 gcttgagcca cttgaggctg aggatggcct ccagacgctg cgcatcatgg ttggccagca
    18781 acgtcaggcc gaccaccagc ggaataatcc ccaagccggc caatatcacg gctggccgcg
    18841 ccgaacgcca gtggcgcaac acatacagca aggtgaacgg cgggatgaaa ctggcccagc
    18901 cccacagcag gccggtagca aaggcgcgca tcaccagcca gaccaacccg gcgagtatca
    18961 gcagcaagcc acccagaatc agcagcgcat ccattcagta ttccttagcg atggttaaac
    19021 gagtggttat ccgccggacc ggcatacctc aaggctcatc gaccttaaag tgcgcccgcg
    19081 ccgtggcaat cggctcggtc tggctggttt gccaggcggt gatggccacg ttggcgaccc
    19141 gacgcccctg acgccacacc tggcattgca cgaaggtgtc acgataatgg ccagcgcgaa
    19201 ggtaatctat cgagaagtcg atgattttcg gcaaatgtgg aatgcccata aacatcagca
    19261 ggtggagcat cgccgaatgt tccataaagc cggcgatcac cccgccatgc aatgccggca
    19321 aggtaggatt gccgatattg tcctggttgg ccggcaggcg aaataccatg tcatcgccga
    19381 ggcgcagaca ctcgatgcca agcaacttgg catagggaat cagactgatc agtgggtcgt
    19441 agttattctg ctcgtgggct tcgcgcacca gctggttgag gctcagcgtg ctcattggcc
    19501 agctcctttg gcagtgcgtt gcatatcggc gggcttaccc atacgcataa acgcaccaac
    19561 cacatgggca atcggctcat cggggttgtc ctgataggca taaccacggg tgaagatgat
    19621 gttctcggtc acccggtagc actcggcaaa accaaataca tccttgttcg gctcggccgg
    19681 gcgcatgtaa tcgatgcgca ggtcgagggt cgggcagatt tcgaactcag gcagcacaca
    19741 cacggtagaa atgccgcagg tggtgtccat caacgtggtg atggcgccgc cgtggatcac
    19801 tccggtttcc gggttgccaa caatctgcgg gctgtagggc aaccgcaggg tcagcccctc
    19861 agcggtggcg tcctgcacct gcaggcccag cacctggcaa tggcgcagta ccgagagaaa
    19921 gcgctgggcg cgctctagaa cagggttggt gctcatcatg aagcctcgag atgcctagct
    19981 aaaaccgcgc atgatagcgc tgagcaccca tgaacggcac acacgcatac gcttaatatg
    20041 atctgaactt attggcgctt ggctctctcg aaaaactgta atggcatttt ttccaaggag
    20101 aacaccatgc aaaaaacagt cgcttatgcc ttcatgctag ccgccacact gggccttgcc
    20161 gcgtgcgaca agccttccga ggatactcgc gaggacgccc gcgaagctca atcggaagcc
    20221 caggaatcga tgaacgatgc ggctgagcaa agtaatgaag ctgccaacct agaggctgaa
    20281 gccgctcggg aacgcgctga agaaaaccgc gaggaagccg aggacgcgaa cgagccgatg
    20341 cctacggccc cgatcacgcc aaccccggcg ccaaccgatc aaaccaatca gtaaccgcta
    20401 ctgatttgcc cagcccgcag tctagctgcg ggctttgttt tcctgccgtc aggaacttcg
    20461 cttcgcttga tcgctccaag tagcacggat gaggccacat gccatcatcc gcatcacaca
    20521 tggagacgta accgtgcaaa aacctttcac ttatgccctg cttcttgccg ccatgctcgg
    20581 cctggctgct tgcgagaaaa ccccggaaga caaactagag tcggccaagg aatccgctgc
    20641 tgaagccatc gagtccatgg gcgacgctgc tgaagaaacc ggcgaagcca tcgagcagaa
    20701 agccgatgaa gtgatgggca ccgagccgac cactggcgag aaaatcgaag acgctgccga
    20761 tgatgcggct gatgccatca gcgatgctgg cgatgaagtc aaagatgctg tcgacggcca
    20821 ataagccttc cagctgaaac aaacaggccc gcttaatgcg ggcctgtttt gtttatggcg
    20881 tgatcaaagc gccggggtaa gcatcagcag caggctgcac aacagcccaa ccacccagac
    20941 cacgctgcgc tgccagtgca tatcagccca gtagaacgcc agatacaaca cccgcgcaac
    21001 gacgaaggtc accgccaata tatccaccag aatgccgtgg ttgttggtca cgtgcgccat
    21061 caacacgccg acggcaaaca gcggaaacgc ctcgaagctg ttcaggtgcg ccgccagggc
    21121 acgcgcgcca aagcctgtca gacgcgcttg ctgggcgcgc gggtggtggt tgtcgtagtg
    21181 accgtcaccc tccttcgcca tggccttggc caccggggcc ttggcaataa agatcaataa
    21241 tgcgctgata aaaatgcacc agaatggaat gctcattcgc ttttctcctt gggcgattcg
    21301 aacgccgggg tctgggtgta gaccatgact tccagtacat cggaatgaaa ctcgcggcgg
    21361 tacaacacca ataccacacc tgcgctgacc agcatgaaaa accatgggtt gataaaccag
    21421 gacagggttg ccatgccgaa gtaataggcg cgcaaaccct ggttgaactg gttggcggcc
    21481 atcgagatca cccgtgcggt gcgttcggca aaggctttgc gctcctgctc cgtgacatgt
    21541 cgctcaccca ccatcggcgc agagcccacc agtaaggcag cgaagttgta ctgacgcatg
    21601 caccagctga aggtaaagaa ggcgtagacg aacaccactc ccaggcacag cagtttgacc
    21661 tcggacagct cgcggctggc ggcctggaca aagggcagat cagccagcag cgatactgcg
    21721 cgatcagagg cgccaagcac ggtgagaata ccggcgagga tgatcagcgt actggaggcg
    21781 aagaacgaag cgttgcgctc caggttgccg atcacgttgg catcagcgat gcggttgtcg
    21841 cgcagcaaca tgcggcgcat ccagtcttcg cggtacaggt gtagcacgct ggccagacat
    21901 ggcgtggtgc ggcccttggc ctgggcataa cgggtgtaac cggcccagca gacgacgaac
    21961 cagagaacgg caagcaggtg cggtaggtag tagtcactga ttggcatgca aactccttgt
    22021 taacctgcgc cgattataga caggcctggc tttcagctgg ccaacaaaaa ggccgctcag
    22081 tgagcagcct tctataaaga gcaaaactta gactgcggcg gttttcggcg cacccagcac
    22141 gcgatccacc acaacagcca ggaccagggt cagcagcacc ggcaacagcc agccgaggct
    22201 ttgatccgcc agcggcaact ggctgaagaa gcttgggatc agcgccttgc cgccggctgc
    22261 ggcaatcccg tcaaccaggc caaacaccag ggtgaccgcc atcaccgggc ggaacacccg
    22321 cgccggcgaa caccacagat catccaacag gctcagcgcc accagcacga tggccagcgg
    22381 atacaagccg accagcaccg gtatagagaa gctgatcagc tgtgtcagac cctggttggc
    22441 caccagcagg ctgaacaaac caaacgcgat caccaccgtg cggtaggaaa ccggcaacag
    22501 gctgctgaag aactcgccac aggcggtcag caaacccaca gccgtggtca agcacgccag
    22561 ggtaatgacc acggccagta gcaggctacc ggcagtaccg aaggtgtgct gaacataggc
    22621 ggtaagcacc tgcacgccat tctgcgcttc accggcaata ccctggctgg tagcgccgag
    22681 gtagaacagc gccagataga ccagcgacaa gcctactgcg gcgatcaggc cggcaatcac
    22741 cgagtaacgg gtaatcagct tgccatcggt cacgccatgg tcacgaatcg cagtggcaat
    22801 cacgatgccg aacaccagcg cgccgagggt atccatggtc aggtaaccct gcaggaaacc
    22861 ctgcacaaat ggcgcgctct gataatccgg cgaaacctgg ccgatctccc ccgccgggat
    22921 cagcagcgca gcgccgccca ataccagcag cgccgccagc agcaccgggg tgatgtactt
    22981 gccgatgcga tccaccagtt gcccaggatt cagcgacagg aacagcaccg cagcaaagta
    23041 ggccaaggta tagagcaaca gcgccgtact gctattgccg ctgaaaggcg cgacgcccat
    23101 ttcgaacgac accaccgcgg tgcgcggcgt ggcaaacagt ggaccgatgg ccaggtacac
    23161 cgctaccgca aacagcacgc cggcgcgctt acccagcggt gcggtgagca gcgccatacc
    23221 gccaccgacg cgcgccaggg caacaatggt cagtagcggc aaaccgacgc cggtgagcag
    23281 aaagcccaac gccgccaggc cgatattctc gcccgccgcc ataccggcgc tgggcggaaa
    23341 gatgatgttg ccggcgccga ggaacagcgc aaacgtcata aagccgaggg caaggagatc
    23401 cgagcctttc aaacgagtca tagaggggaa taccacaggc agaaagttgc aagttgaaaa
    23461 ccgaaagttc cgagggtttc ccgatggatc gggggaaacg tcacccagcc ggttaagcgg
    23521 ggccactgca gcacccggag ccgcttgtgg cggagcaggc gctaatagtt gcaagcctaa
    23581 cgaaattgac gcgcaagcgg cactggcaca ggtcaggctg tcggactggt gaaaaaagct
    23641 gtcgcatcga caacagttca tccctggccg caataaaaaa ggccacccga cggtggcctt
    23701 ttctcaggtc aaaaagcctt aggctttttt cacttcccaa ccggtcagct cggccagggc
    23761 cttgccgatg tcagccaggg aacgcacggt tttcacacca gcgtcttgca gggctgcgaa
    23821 cttctcgtct gcagtaccct tgccgccgga gatgattgcg ccggcgtggc ccatacgctt
    23881 gcccgcagga gcggttacac cagcgatgta ggacactact ggcttggtca cgttggcctt
    23941 gatgtaagca gcagcttctt cttcagccga accgccgatc tcaccgatca tcacgatcgc
    24001 ttcggtcttc gggtcttcct ggaacagctt caggatgtcg atgaagttgg agcccgggat
    24061 cgggtcaccg ccgatgccga cgcaagtgga ctgaccgaaa ccggcgtcag tggtctgctt
    24121 aacggcttcg taagtcaggg tgccggaacg cgacacgatg cctactttgc ctggcaggtg
    24181 gatgtgaccc ggcatgatgc cgatcttgca ctcgcccgga gtgatcacac ctgggcagtt
    24241 agggccgatc aggactacgc ccagctcgtc gcacttaact ttagcgtcca gcatgtccag
    24301 ggtaggaatg ccttcggtga tgcacacgat cagcttgatg ccgccgaatg ctgcttccag
    24361 gatcgagtcc ttgcagaaag gagccggaac gtagatcacg ctggcggtgg cgccagtggc
    24421 agctacagcg tctttcacgg tgttgaacac tggcaagccc aggtgctcgg tgccgccttt
    24481 gcccggagtt acgccgccaa ccatcttggt gccgtattcg atggcttgct gggtgtggaa
    24541 actaccttgc gaaccggtaa tgccctggca gataactttg gtgtctttat tgatcaggac
    24601 gctcattatt tgccctccgc agctttgaca acttgttgag cagcgtcggt caggctggta
    24661 gccgcgatga tgttcaaacc gctttctgcc agtactttag cgcccagttc agcgttgtta
    24721 ccttcaaggc gcacaacaac cgggattttc acgccaactt ctttcactgc accgatgatg
    24781 ccttcggcaa tcatgtcgca acgaacgatg ccgccgaaga tgttaaccag tactgcagcg
    24841 acgttggagt cggacaggat gatcttgaac gcttcggtaa cgcgttcttt ggtagcaccg
    24901 ccgcctacgt cgaggaagtt ggctggtttg ccgccatgca ggttgacgat gtccatggta
    24961 cccatggcca ggccagcacc gttgaccatg cagccgatgt tgccttccag ggctacgtag
    25021 ttcagttcga acttggcagc gtgggcttcg cgcggatcgt cttgcgacgg atcgtggaaa
    25081 gtcttcagct taggctgacg gtacatggcg ttggcgtcga tgttgatctt ggcgtcgagg
    25141 cagtgcagat cgccgtcagc cttgatcacc agcgggttga cttccagcag ggccagatcg
    25201 tggtcctgga acagcttggc cagacctacg aagatcttgg cgaactgggc aacttgcttg
    25261 ccttccaggc ccagctggaa agccagctca cgaccctgga atggctgagc gccaaccagt
    25321 ggatcgatag tggccttcag aattttttct ggggtttcgt gagcgatctt ctcgatgtcc
    25381 acgccacctt cggtggaagc catgaacacg atacgacggc tcgaacggtc aacgaccgcg
    25441 cccaggtaca gttccttggc gatatcggtg caggactcaa ccaggatctt ggttaccggc
    25501 tgaccgttgg cgtcagtctg gtaagtcacc agacgcttgc ccagccagtt ggcggcgaag
    25561 gctttggcgt cttccttgct cttgaccagc ttcacgccgc ccgctttacc gcggccacca
    25621 gcgtggactt gagccttgac gacccactca gtgccaccga ttttgtcgca ggctgctgcg
    25681 gcgtcttcgg gggtgtctac ggcgaaaccc ttggatacgg gcaggccgta ttcagcgaac
    25741 agctgcttac cctgatactc gtggagattc atgcttgtct accgtcttcg tttaggtatt
    25801 gcgcatacgg tgctgcactt gtgatgccgc accacctgtg actgcttc
//
LOCUS       DASRUM010000075         5306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_539869, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000075 DASRUM010000000
VERSION     DASRUM010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539869"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2500
                     /locus_tag="VFF22_05365"
     CDS             <1..2500
                     /locus_tag="VFF22_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007943333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZX16276.1"
                     /translation="EQRLDSEYLFFDAQLQPISRRQNLKETYDPRSRPWYNSALQSQK
                     HFTTAPYAFFSTQEIGTTIARKSSETTVVAADLTLDDLSATLAQHRLTPSTEVVLYSP
                     DGSAVAYPDSSRLVIPGEKLQLAQVKDLSPVLADFLNLGLDKDRQGAVRLNKQRWQIS
                     YSQFTEGGPQGLRLALLAPESELLADAYRIRWQGAVLTLGILLLCIPLGWLLSNILVK
                     PLRLLVGEAEAIRSFNFDYPSSGRSFVKEVDQLAVSMGKMKDTISSFLDIAATLSAET
                     RFDALLRRVLRETVDLSEASGGLLYLRDSNSGRLEPHGLFINELQHNLEEHRIPSFDS
                     DDPALPSWLAQTATGGSSVTVALGFEHAQGFQSLLHTLDSPRAHLVCTGLHNRQGQTL
                     GVLVLLHRDTGDEADLAMLRPERIAYVEAVSGVAALCIESQRLLDQQKKLLDAFIQLI
                     AGAIDAKSPYTGGHCQRVPEITLMLARAAAASDEAPFHDYQPTDEEWEALHIAAWLHD
                     CGKVTVPEYVVDKATKLETIYDRIHEVRMRFEVLKRDAWISYWENLSQGGNADSLGQV
                     RDELLATLDDEFAFIARCNLGGEAMADADIERLQSIATRTWTRTLDDRLGVSWEENLR
                     QSRTPAPALPVQEALLSDKAEHLIERPESELIAEDNPWGFKLEVPHYKHNRGEVYNLG
                     ISRGTLTNEERFIINGHMVQTIRMLSHLPFPAHLANVAEIAGGHHEKMDGTGYPKRLK
                     REDMSLPARMMAIADIFEALTAVDRPYKKGKLLSESLNIMAGMCKGAHIDPELFGLFV
                     REKIYLSYAERFLRREQIDQVDNDALLAKAGLSD"
     gene            complement(2562..2903)
                     /locus_tag="VFF22_05370"
     CDS             complement(2562..2903)
                     /locus_tag="VFF22_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HZX16277.1"
                     /translation="MATNRSRRLRKKLCVDEFQELGFELNLNFKQDLADDAIDSFLEQ
                     FLREAMTANGLGYVGGDDFGLVCLSKRGSVNEEQRAAVEAWLKGRDELVDFTASPLID
                     VWYPDNSINQA"
     gene            3138..4586
                     /gene="dacB"
                     /locus_tag="VFF22_05375"
     CDS             3138..4586
                     /gene="dacB"
                     /locus_tag="VFF22_05375"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HZX16278.1"
                     /translation="MIKTLRHLAIAGLLLPVCLPLAAAPSGISNKVQQSLKANKISSQ
                     SLSVVTVPLNGPGSSTFFNADISVNPASTMKLVTTYAALELLGPTHQWKTEFFTDGQL
                     KNGVLNGNLYLKGGGDPKLNMEKLWLMMRDLRANGVLQVTGDLVLDRSHFVQPQLPSF
                     NDDGGDANKPFLVAPDSLLVNLKALRFIARTEGGKVHIAAEPPIADIRIDNRVKALPA
                     AKCPGWPDVRYNPVTEFDGTTVIVSGKLAEGCSSQTYLSLLDHPSYAAGAIRAIWQEL
                     GGSIMGKDRLADVPEKARLLARAYSPDLTEIIRDINKYSNNTMARQLFLSIGAQFRNS
                     ADQDDSKAAQRVIRSWLARKGITAPHLVMENGSGLSRAERVSAREMATLLQAAWKSPY
                     AAEFMASLPLVAMDGTMRKRLHRTPLAGEARIKTGTLNNVRAIAGFSRDSNGNSWAVV
                     AILNDPRPWGASSILDQVLIDLYKQPKLSAQR"
     gene            complement(4621..5094)
                     /locus_tag="VFF22_05380"
     CDS             complement(4621..5094)
                     /locus_tag="VFF22_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZX16279.1"
                     /translation="MADKKVSTEIDLSSVSAFELPLFQALRRLQQAGEVHAKRLSRFG
                     GLTPMQLMILQVLAGETRLTASDLSGRVSLTAATLSGMLDRLEEKGLLQRQRDDQDRR
                     RQWLLLSEQGRTLLQQAPSLLPPEFVERFAGLEEWERHSLTAALLRAAQLCGEME"
     gene            5206..>5306
                     /locus_tag="VFF22_05385"
     CDS             5206..>5306
                     /locus_tag="VFF22_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16280.1"
                     /translation="MCAIAGELRFDQRPADLAAVERITHHMAARGPDA"
BASE COUNT         1114 a         1744 c         1495 g          953 t
ORIGIN      
        1 cgagcagcgc ctggattctg aatacctgtt cttcgacgcg cagttgcagc ccatcagccg
       61 ccggcagaac ctcaaggaaa cctacgaccc gcgtagccgt ccctggtaca acagcgcact
      121 gcagagccag aagcacttca ccaccgcgcc ctatgccttc ttctccaccc aggaaatcgg
      181 caccaccatt gcccgcaaaa gcagcgaaac cacggtggtg gccgccgatc tgaccctcga
      241 cgacctttcc gccaccctgg cccaacatcg cctgacgccc tccaccgaag tggtgctgta
      301 cagccccgat ggcagtgccg ttgcctatcc agacagctcg cgtctggtaa tccctgggga
      361 aaagctgcaa ctggcgcagg tcaaggatct cagcccagta ctcgccgact tccttaacct
      421 cggcctcgat aaggatcgcc aaggcgcagt gcgcctgaac aagcaacgct ggcaaatctc
      481 ctacagccag ttcaccgagg gcggcccgca gggattgcgc ctggccctgc tggccccgga
      541 aagtgaactg ctggctgatg cctaccgcat ccgctggcag ggcgcggtgc tgaccttggg
      601 cattctgctg ctgtgcatcc cgcttggctg gctgctctcc aatattctgg tcaaaccgct
      661 gcgcctgctg gtgggcgaag ccgaagccat ccgcagcttc aatttcgatt acccgtccag
      721 cggccgctcc ttcgtcaagg aagtcgacca gttggcggtg tcgatgggaa agatgaagga
      781 caccatctcc agcttcctcg acattgccgc cacgctctcg gcggaaaccc gcttcgatgc
      841 cctgctgcgc cgggtgctgc gcgaaaccgt cgaccttagc gaagccagtg gcggcctgct
      901 ttatctgcgt gacagcaaca gcggccgcct cgaaccacac ggcctgttta tcaacgagct
      961 gcagcacaac ctggaagaac accgcatccc gagcttcgac tcagacgatc ccgccctgcc
     1021 cagctggctc gcgcaaacgg caaccggcgg ctccagcgtc accgtggcac tgggcttcga
     1081 gcatgcccag ggcttccaaa gtctgctgca caccctcgac agcccacgcg ctcacttggt
     1141 ctgtaccggc ctgcataacc gtcagggcca gaccctgggc gtgctggtgc tgctgcaccg
     1201 cgacaccggc gatgaagccg acctggccat gctacgcccg gaacgcatcg cttatgtcga
     1261 ggcggtttcc ggcgtggcgg cgctgtgcat cgagagtcag cgccttctcg atcaacagaa
     1321 aaaactgctt gatgccttta tccagctgat cgccggcgcc atcgacgcca agagccccta
     1381 taccggtggc cactgccagc gcgtgccaga aatcaccctg atgctcgccc gcgccgctgc
     1441 ggcaagcgac gaagcgccgt ttcacgatta ccagccaact gacgaggaat gggaagccct
     1501 gcatatcgcc gcctggctgc acgactgcgg caaggtcacc gtgcccgagt acgtggtgga
     1561 caaggccacc aagctggaaa ccatctacga ccgcatccat gaagtgcgca tgcgctttga
     1621 ggtgctcaag cgtgatgcct ggatcagtta ctgggaaaac ctcagccagg gcggcaacgc
     1681 cgacagcctc ggccaggtgc gcgacgagct gctcgccacc ctggatgatg agttcgcctt
     1741 tatcgcccgc tgcaacctgg gcggcgaggc gatggcagac gccgatattg agcgcttgca
     1801 gagcatcgcc acgcgtacct ggacgcgcac tttggatgat cgcttggggg tttcctggga
     1861 agagaatctg cgccagtcac gcacgccggc gccggcgtta ccggtgcagg aagcgctgct
     1921 cagcgacaaa gccgaacacc tgattgagcg ccccgaaagc gagctgatcg ccgaagacaa
     1981 tccctggggc ttcaagctgg aagtgccgca ttacaaacac aaccgcggcg aggtctataa
     2041 cctcggcatt agccgcggca ccctgaccaa cgaagagcgc tttatcatca acggccatat
     2101 ggtgcagacc atccgcatgc tcagccacct gcccttcccc gcgcacctgg cgaacgtcgc
     2161 cgagatcgcc ggcggtcacc atgaaaagat ggacggcacc ggctacccaa aacgcctgaa
     2221 gcgcgaggac atgagcctgc cggcacggat gatggcgatt gccgatatct tcgaagcgct
     2281 gaccgccgtg gaccgtccct acaaaaaggg caagctgttg tccgaatcac tgaacatcat
     2341 ggccggtatg tgcaaaggcg cgcacatcga ccccgaattg ttcgggctgt ttgtgcggga
     2401 aaagatctac ctcagctacg ccgaacgctt tctacgtcgt gagcagattg atcaggtcga
     2461 taacgatgcg ttgctggcca aggctggcct gagcgactaa cgaggaagta cagaagggaa
     2521 aagcaacagg gcatgcgcga tgcatgccct gcttacaggt tttaggcctg attgatcgag
     2581 ttgtctgggt accagacgtc gatcagcggg ctagcggtga aatccaccag ctcgtcacgg
     2641 cctttcagcc aggcttcaac ggcagcgcgc tgctcttcgt tgaccgagcc acgcttggac
     2701 aggcacacca gaccgaagtc atcgccgccg acatagccca ggccgttagc ggtcatggct
     2761 tcacgcagga actgctcaag aaaactgtcg atggcgtcat cggccaggtc ttgcttgaag
     2821 ttcaggttca gctcaaaacc cagctcctgg aattcatcca cgcagagttt tttgcgcaga
     2881 cggcgggaac ggttagtagc catggaacaa tcctcaaaag tgataacgcg cggcactcta
     2941 gcagtttcgc ggcctgcgcg cccgcttcga agacgacagg tcgctcgatt gcccgcagaa
     3001 aagccctacc gatggcacta acggccactc ggccggtcct gtattggggc ataattgccg
     3061 acattatccc aacccgcgag gggacctctt tacatgtctc gcgtcatctt tctccctcag
     3121 ctggagggtc gttacccatg atcaaaaccc tgcgtcacct ggccatcgct ggcctgctat
     3181 tacccgtttg cctgccactg gcagccgccc cgagcggtat ttcaaacaag gttcagcagt
     3241 cgctgaaagc caacaagatc agcagccagt cgctgtccgt ggtcacagtg ccgctcaatg
     3301 gccctggcag cagcaccttt ttcaatgccg atatttccgt taacccggct tccaccatga
     3361 agctggtcac cacctacgca gcgcttgagc tgctgggtcc gacccaccag tggaaaactg
     3421 aattctttac cgacggccag ctgaagaacg gcgtgctcaa cggcaacctg tacctcaagg
     3481 gcggcggcga ccccaaactg aacatggaaa agctctggct gatgatgcgc gacctgcgtg
     3541 ccaacggcgt gttgcaggtg actggcgacc tggtgctcga ccgcagccac ttcgtacagc
     3601 cgcagctgcc cagcttcaat gacgacggcg gcgacgccaa caagcccttc ctggtggccc
     3661 ccgactcgct actggttaac ctcaaggcgc tgcgttttat cgcgcgcacc gagggtggca
     3721 aggtgcatat cgccgccgag ccgccgattg ccgatatccg catcgacaac cgcgtcaaag
     3781 ccctgcccgc agccaaatgc ccgggctggc cggatgtgcg ctacaacccg gtaaccgagt
     3841 tcgatggcac cacggtgatt gtcagcggca aactggccga gggctgcagc tcacagacct
     3901 acctgtcgct gctggatcac ccgagctatg ccgccggcgc catccgcgcc atctggcagg
     3961 aactgggcgg cagcatcatg ggtaaggatc gcctcgcaga tgtgccggag aaggcccgcc
     4021 tgctggcacg cgcctactcg ccggacctga ccgagatcat ccgcgacatc aacaagtaca
     4081 gtaataacac catggcgcgg cagctgttct tgagcatcgg cgcgcagttt cgcaactccg
     4141 ccgatcagga cgatagcaag gccgcgcaac gggtcattcg cagctggctc gcgcgtaaag
     4201 gcatcaccgc gccgcatttg gtgatggaaa acggctccgg gctgtcccgc gccgagcggg
     4261 tcagtgcgcg ggaaatggcg acactgctgc aagcagcctg gaagagcccg tacgccgcag
     4321 agtttatggc ctcgctgcca ctcgtggcga tggacggcac catgcgcaag cgcctgcacc
     4381 gcacgccact ggcgggcgaa gcacggatca agaccggtac gctgaacaac gtacgcgcca
     4441 tcgccggttt cagccgcgac agcaatggca acagttgggc cgtggtggcc atcctcaacg
     4501 acccacgccc atggggcgcc tcgtcgattc tcgatcaggt gctgatcgat ctgtataagc
     4561 agcccaagct cagcgcccag cgctaaggca gcaacgccgc gcctacctgg cgcggcgttt
     4621 ctattccatc tccccgcaca gctgcgccgc gcgtaacaac gcggcggtca ggctgtggcg
     4681 ctcccactcc tccaatccgg caaaacgctc gacaaactcc ggcggcagca ggctcggcgc
     4741 ttgttgcagc agcgtgcggc cctgctcgct aagcagcagc cactgccggc gccggtcctg
     4801 atcgtcgcgc tgacgttgca gcaagccctt ttcctccagc cggtcgagca tgcccgatag
     4861 ggtcgcagcc gtcaggctga cgcggccact cagatcgctg gccgtcagac gcgtttctcc
     4921 tgctagcacc tgcagaatca tcaattgcat cggcgtcagc ccaccaaagc ggctgagccg
     4981 tttggcatgc acttcaccgg cctgttgtaa acgccgcaac gcctgaaaca gcggcagctc
     5041 gaaggccgaa acagatgaca aatctatttc agtagaaacc tttttatcag ccatacatgc
     5101 cttttaattc gatataaaac tttgacataa aagcattatt ttgttttggt gtaaaaggtt
     5161 cggctgcccc gaaccactcc ccaacggcaa taccggtaag gaattatgtg cgcaatcgct
     5221 ggagaattac gttttgatca acgaccggcc gacctggctg cagtcgagcg aatcacccac
     5281 cacatggccg cccgcggccc cgatgc
//
LOCUS       DASRUM010000076        10752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2378043, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000076 DASRUM010000000
VERSION     DASRUM010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 10752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10752
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2378043"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1195
                     /gene="asd"
                     /locus_tag="VFF22_05390"
     CDS             83..1195
                     /gene="asd"
                     /locus_tag="VFF22_05390"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HZX16281.1"
                     /translation="MKRVGLIGWRGMVGSVLMQRMLEEQDFDLIEPVFFTTSNVGGQG
                     PAIGKETAPLKDAYSIDELKSLDVILTCQGGDYTSEVFPKLREAGWQGYWIDAASSLR
                     MDDSSVIVLDPVNRKVIDQQLDAGTKNYIGGNCTVSLMLMALGGLYEAGLVEWMSAMT
                     YQAASGAGAQNMRELIKQMGAINASVADELADPASAILDIDRKVAEAMRGESFPVDNF
                     GVPLAGSLIPYIDKELPNGQSREEWKGQAETNKILGRFKNPIPVDGLCVRIGAMRCHS
                     QALTIKLNKDVPLSDIEGLISQHNPWVKLVPNQREASIRELGPTAVTGTLSVPVGRLR
                     KLNMGSQYLGAFTVGDQLLWGAAEPLRRMLRILLER"
     gene            1340..2350
                     /locus_tag="VFF22_05395"
     CDS             1340..2350
                     /locus_tag="VFF22_05395"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018928095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HZX16282.1"
                     /translation="MTRTFDIAVIGATGSVGETLVQLLEEREFPVANLHLVASGESAG
                     RSLSYKGKNLRVRDLETFDFASVRLVFLAAGSKVTQKYAAQAAAAGCAVIDLASGLPA
                     AQAPRVIPEVNPEALASLATPYVLTSPSAPGIALAAVLAALPEQASIRRVTVTACLAV
                     SSRGREGVSELARQTAELLNGRAFEPQLFDRQMAFNLLAQVEQTDSAGHAALERQIAA
                     ELKELLGLPELQVAVTCIQVPVFFGDSLSVSLQTEAAIDLKSVYAALDAAPALELVEL
                     DDYPTAVGDAVGQDVVYVGRVRAGLSDPAELNLWITSDNVRKGAALNAVQLAELLIKH
                     YL"
     gene            2505..5324
                     /locus_tag="VFF22_05400"
     CDS             2505..5324
                     /locus_tag="VFF22_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HZX16283.1"
                     /translation="MVRVRKLVLAIAAASALSSGMAHALGLGEVTLQSALNQPLVAEI
                     ELLEVRDLASNEVIPTLASPEAFTKAGVDRQFFLTDLKFTPVLKPNGKSVIRVTSSKP
                     MREPYLNFLVEVLWPNGRLLREYTLLLDPPLYTPQSVIPATARLPVAAPAPAPRVQAP
                     APTPRPATAAAQPAAPTAPAAPAAPAAKPLQGNEYKTTANDTLWEIAQRARAGGSVHQ
                     AMLAIQDLNPDAFIGGNINRLKSGQVLRLPDEQQVKSRAQGEAIAQVAEQNTAWREGR
                     SVAASARQLDATKRTSAAATPAKLETGDSLKLVAADSGKATAGSDKGAADSKALTDKL
                     AVTQESLDSSRRENDELKGRMTDLQSQLDKLQRLIQLKDDQMAKLQADLAAQGQAPAV
                     APAVPAAPVAETPAAPVEPAPEVAPQDPAAPAAPAAPAATGETDFNYSEEPEAAAPVT
                     APEPAPAAAPEPAPVTPVAPVVEAPVVAEPQAESSTMDDLLANPMLLGAAGGGALLVL
                     LIALMMLSRRNALKEAELQESLAADDGSSDLGAGLDLPDDSFAGVADDLDGQQADLPN
                     EERVTAQTGDALGEADIYIAYGRFPQAAELLQGAINDEPQRGDLRLKLMEVYAEMGDR
                     NGFARQDNELREIGGSAAELDQIKARYPAMAVAAGAGFAAAAAEENLDSFSLDDLALD
                     EPSPKAATGDQDDAFDLSLDDLESELDRDLQAAGNTASLDDLDSLSLDDDLDFSSPVE
                     QANNKDDDLGFDLSLADSGDSGLDLSGDLADFSLDLDEPAKPAAAAEDDFLLSLDDEP
                     SAAPAVAESSFDLPDEQAGGDFDLSADFDLSLNDEAPAQPSSDSFAAQLDEVSAELDL
                     LSDDLDQPADLGMPASAASSSMDNDDDFDFLSGTDETATKLDLARAYIDMGDTEGARD
                     ILDEVITEGNEGQQNEARELIAKLV"
     gene            5328..6194
                     /gene="truA"
                     /locus_tag="VFF22_05405"
     CDS             5328..6194
                     /gene="truA"
                     /locus_tag="VFF22_05405"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HZX16284.1"
                     /translation="MSDGLTTTAADSAAVGVFKIALGVEYKGSRYRGFQRQRAGVPSI
                     QESLEKALAKVAGGVPVTLSCAGRTDALVHASAQVVHFNTPVDRSMHAWVMGANMNLP
                     GDISVTWAKSMPAHFDARFCAQARRYRYVIYNDQIRPAHMAEEVTWNHRPLDVERMRQ
                     ASRAFIGTHDFSAFRARQCQAKSPIKTVHHLELLQYGRFIVLDIRANAFLHHMVRNIA
                     GVLMTIGTGERPVEWAQEVLETRVRRTGGVTAHPYGLYLVQVDYPEEFDLPKRYLGPH
                     FLSGLPDLAASS"
     gene            6302..6922
                     /locus_tag="VFF22_05410"
     CDS             6302..6922
                     /locus_tag="VFF22_05410"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017906546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HZX16285.1"
                     /translation="MSAVRSKICGITRIEDALSAVEAGADAIGLVFYAKSPRAVTLQQ
                     ARSIIAALPPFVTTVGLFVDASRCELGEILDAVPLDLLQFHGDESPEDCDGYHRPYIK
                     ALRVKPGDDIAAQVALYKNASGVLLDTYVPGIPGGTGEAFDWSLVPEGLSKPVILAGG
                     LTAENVAQAIVQVRPYAVDVSGGVEMAKGIKDAAKIRAFMQAVKAA"
     gene            7140..8024
                     /gene="accD"
                     /locus_tag="VFF22_05415"
     CDS             7140..8024
                     /gene="accD"
                     /locus_tag="VFF22_05415"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HZX16286.1"
                     /translation="MSNWLVDKLIPSIMRSEVKKSSVPEGLWHKCPSCDSVLYKPELE
                     KTLDVCPKCNHHMRIDARSRLDIFLDAEGREELGADLEPVDRLKFRDSKKYKDRLTGA
                     QKQTGEKDALVAIRGTLEGMPIVTCAFEFSFMGGSMGAIVGERFVRAANAALEQRCPL
                     VCFSASGGARMQEALISLMQMAKTSAVLARLREEGIPFISVLTDPVYGGVSASLAMLG
                     DVIVAEPRALIGFAGPRVIEQTVREKLPEGFQRSEFLLEHGAIDMIIHRAELRPRLAR
                     LLAQFMGLPSPVALPATA"
     gene            8021..9328
                     /gene="folC"
                     /locus_tag="VFF22_05420"
     CDS             8021..9328
                     /gene="folC"
                     /locus_tag="VFF22_05420"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019649330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HZX16287.1"
                     /translation="MTERSLADWLSYLEQLHPSAIDMGLERSRVVLQRLGLTKLAPRV
                     ITVTGTNGKGSTCAFIASLLQGQGLKVGVYSSPHLLRYNERVQIAGVEATDAMLCEAF
                     AVVEAARGETSLTYFEMGTLAAFWLFQQAGLDALVLEVGLGGRLDAVNLLDADIALVT
                     SIGIDHADWLGDTRESVAFEKAGIFRAGKPALCGDLEPPQPLLDQVALFDAPFFLRGR
                     DYDLSINAQDWSWYGLNHQGEVLSLAHLPLLDLPMENAALALQAYAVMQLPWQPEVIS
                     QALQRTRVTGRLDRRMLNWQGKSLTLLLDVGHNPHAAEYLAQRLASRALSGKRLAVFG
                     LLADKDLPGVVAPLLSEVDSWAVAALPTSRTRAAAELQAHLHNSGAQVSAYASVQAAL
                     EAQCEQASDGDEVLLFGSFYCVAEALDWLARQANGGVQDGFAG"
     gene            9312..9920
                     /locus_tag="VFF22_05425"
     CDS             9312..9920
                     /locus_tag="VFF22_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZX16288.1"
                     /translation="MALLDKGLKQRMVGALVLLALAVIFLPMLLSREDESQRVQVDAP
                     AMPAAPEAPEVELQPVAVPEPQLLPDEPIGSEAAQSLEPSEPVVSELPAEPITPAAPA
                     AAPEPVAKPPQPAPSKLDANSLPISWSVQLASLSSSDGAQKLQKTLRSQGYNAYIRSV
                     DGMNRVFVGPLIERAEADRLRDQLNRQHKLNGFVVRFQPEAS"
     gene            10069..10623
                     /locus_tag="VFF22_05430"
     CDS             10069..10623
                     /locus_tag="VFF22_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009403101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HZX16289.1"
                     /translation="MAFTWVDWAIIAVVAVSSLISLSRGFVKEALSLLTWIVAGVVAW
                     MFGGALSQHLVDFIETPSARVIAGCAILFVVTLLVGALVNYLIGELIRVTGLSGTDRF
                     LGMVFGAARGGLLVVVLVGLISLAPVQQDSWWQESALVPHFLMVADWSKNLILGLSSE
                     WLASGVTAPVELPFKEALQQPKLP"
     gene            10669..>10752
                     /locus_tag="VFF22_05435"
     CDS             10669..>10752
                     /locus_tag="VFF22_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16290.1"
                     /translation="MCGIVGIVGKSNVNQALYDGLTVLQHRG"
BASE COUNT         2036 a         2959 c         3401 g         2356 t
ORIGIN      
        1 ggtagtcgaa gcgctgcgta gtctgtaatc ttcctggccc gccggaggtc tccggtggtc
       61 agcttatata ggtgtagtgg ttatgaaacg tgtaggtctg atcggttggc gcggcatggt
      121 cggttccgtg ctgatgcaac gtatgctgga agaacaggat ttcgacttga tcgaaccagt
      181 gttcttcacc acctccaatg tcggcggcca aggcccggct attggcaagg aaacggcgcc
      241 tttgaaggac gcctacagca ttgacgagct gaaaagcctg gacgtgattc tgacctgcca
      301 gggtggcgac tacaccagcg aagtgttccc caagctgcgc gaagccggct ggcaaggcta
      361 ttggatcgat gccgcctcgt cgctgcgcat ggatgacagc tcggttatcg tgcttgatcc
      421 ggtcaaccgc aaggtgattg atcagcaact ggatgccggc accaagaact acatcggcgg
      481 caactgcacc gtcagcctga tgctgatggc cttgggcggc ctgtacgaag ccggtctggt
      541 cgagtggatg agcgccatga cctatcaggc ggccagcggt gctggtgcgc agaacatgcg
      601 cgagctgatc aaacagatgg gtgcgatcaa cgcctccgtg gccgatgaac tggctgatcc
      661 ggccagcgcc attctggata tcgaccgcaa ggtggccgaa gccatgcgcg gcgagtcctt
      721 cccagtggac aacttcggcg taccgctggc cggcagcctg attccttaca tcgacaagga
      781 actgccaaac ggccagagcc gtgaagagtg gaagggccag gcggaaacca acaagatcct
      841 cggtcgtttc aagaacccga tcccggtcga tggcctgtgc gtgcgcatcg gcgccatgcg
      901 ctgccacagc caggcgctga ccatcaagct gaacaaggat gtgccgctgt cggatatcga
      961 aggcctgatc agccagcaca acccctgggt caagctggtg ccgaaccagc gcgaagcgag
     1021 catccgcgag ctgggcccga ccgctgtaac gggcaccctg agtgtaccgg ttggtcgtct
     1081 gcgcaaactc aatatgggtt cgcagtacct cggcgcgttc accgtgggtg atcagctgct
     1141 gtggggcgcg gccgaaccgc tgcgacgtat gctgcgtatc ttgctggagc gttgatcgcg
     1201 ctgcgctgat acacatacag gctgttcaaa aggggcaggc tgttgggtgt tcgagtcaaa
     1261 tcgagcgctc agcagtccgc ttctttgcat ttggcgggtg cagctataca gtgctgggct
     1321 tatttcttcg agccttgtca tgacccgtac ctttgatatt gccgtaattg gcgccaccgg
     1381 cagcgttggc gaaaccctgg tgcagctgtt ggaggaacgc gagtttccgg tggctaattt
     1441 gcatctggtg gccagcggtg agtcggccgg ccgctcgttg tcctataagg gcaagaacct
     1501 gcgcgtgcgt gatctggaaa ccttcgactt tgccagtgtg cggctggtgt tcttggctgc
     1561 cggcagcaag gtgacgcaga aatatgcggc gcaagccgct gcggctggct gcgcggtgat
     1621 tgatctggcc agtggtttgc ctgccgcgca ggcgccacgg gtgatccctg aggtcaatcc
     1681 tgaagcattg gcgagtctgg ccacccccta cgtgttgacc agcccaagtg ctccgggtat
     1741 tgcgctggct gcagtactgg cggcgttgcc tgagcaagcc agcattcgtc gggtgactgt
     1801 gacggcctgt ctggccgtct ccagtcgcgg gcgtgaaggc gtctccgagc tggcgcggca
     1861 gaccgccgag ctgctcaatg gccgtgcgtt cgagccgcag ctgtttgatc ggcagatggc
     1921 gtttaatctg ttggcgcagg tcgagcagac tgacagtgca ggtcacgctg ctctggaacg
     1981 gcagatcgcc gccgagctta aggagttgtt gggattgcct gagttgcagg tagcggtgac
     2041 ctgtattcag gtcccggtgt tctttggcga cagcctgagt gtttcactac aaaccgaagc
     2101 ggccatcgac cttaaaagcg tgtatgccgc gctggacgct gctccggcct tggaattggt
     2161 ggaattggat gattatccaa ccgccgttgg cgacgccgtc ggccaggatg tggtctatgt
     2221 tggccgagtg cgcgcaggtt tgagtgatcc ggccgaactc aatttgtgga ttacgtctga
     2281 taacgtgaga aaaggcgcgg ctctaaatgc tgtgcaatta gccgagttgt tgataaaaca
     2341 ctatctgtaa aagatactta cctagaaatt tgaagaatct ttcgagcttg gcaatactgg
     2401 ctgagttgat tgctgaatgg ggttgcgtct ttgggcgtag caggcagcaa ttttcaacat
     2461 cctctcgctg gtcgagaaaa aaagataaaa caagggataa cgctatggtt cgggttcgca
     2521 aactggtgct ggcaatcgca gctgcttcgg cgctgtcatc cggtatggcg cacgcgctgg
     2581 ggctgggtga agtgaccctg caatcggcgc tcaatcagcc gttagtggct gagattgagt
     2641 tgctggaggt gcgtgacctg gcttctaacg aagttatacc gaccctggcg tccccggaag
     2701 cattcactaa agccggcgtt gatcgtcagt tcttcctgac tgacctgaaa ttcaccccgg
     2761 tacttaagcc caatggcaag agtgtcattc gggtgacatc cagcaaaccc atgcgtgagc
     2821 catacctgaa cttcctggtt gaggtgctct ggccgaatgg ccgtttgctg cgtgaataca
     2881 ccctgctgct tgatccgccg ctctataccc cgcaaagcgt tatccctgct actgcacgcc
     2941 tgcctgtagc cgccccggct ccagccccac gtgtgcaggc tccagctcct acgccgcgcc
     3001 cagcaactgc tgcagcccag ccagcagccc cgacggctcc agcagcacct gctgcgccag
     3061 cagccaaacc gctgcaaggc aatgaataca aaaccaccgc caacgacacc ctctgggaaa
     3121 ttgcccagcg tgcgcgtgct ggcggcagcg tgcatcaggc catgttggcg attcaggacc
     3181 tgaacccgga tgcttttatc ggcggcaata tcaatcgtct gaaaagcggc caggtactgc
     3241 gcctgcctga tgaacagcaa gtgaagagtc gggctcaggg cgaagctatt gctcaggttg
     3301 ccgaacagaa tactgcctgg cgtgaagggc gcagtgttgc cgccagcgcc cgtcagctgg
     3361 atgccactaa gcgcaccagc gctgcagcga ctccagccaa acttgaaacc ggcgacagcc
     3421 taaaactggt tgctgctgat agcggcaaag ccactgccgg cagcgacaag ggtgcagctg
     3481 acagcaaagc gctgactgac aagttggccg tgacccagga aagcctggat tccagccgtc
     3541 gtgaaaacga tgagctgaaa ggccgcatga cggatctgca gagtcagctc gacaaactgc
     3601 agcgcctgat ccagttgaaa gacgaccaga tggccaagct gcaggccgat ctcgctgcgc
     3661 aaggtcaggc acccgctgtt gcgcctgccg tcccagcagc ccctgtagct gaaaccccgg
     3721 ctgctccagt tgagcctgct ccagaggtcg caccacaaga tccagctgca cctgcagcac
     3781 ctgcagcacc tgcagccaca ggtgaaaccg attttaatta cagcgaagag cccgaagccg
     3841 ctgcgccagt tacggcgcct gagccagctc cggccgctgc tcccgagcca gctccggtaa
     3901 cgcccgttgc accggttgtc gaagcgccgg tagtggccga gccgcaagcc gaaagcagca
     3961 ccatggatga tttactggct aacccaatgt tgctgggcgc cgctggtggt ggtgcgctgc
     4021 tggtattgct gattgcgctg atgatgttgt cgcgccgcaa tgcgctgaaa gaagccgagc
     4081 tgcaggaaag cctggccgcc gatgacggct cgtccgatct gggggctggt ctggatctac
     4141 cggatgacag ctttgccggt gtggctgacg acctggatgg tcagcaagcc gacctgccga
     4201 acgaggagcg cgtgaccgcg caaaccggtg atgcgctggg tgaggcggat atctacatcg
     4261 cctatggccg cttccctcag gctgctgagc tgctgcaagg cgcgatcaat gatgagccgc
     4321 agcgcggtga tctgcgtctg aaattgatgg aagtctatgc cgagatgggg gaccgcaatg
     4381 gtttcgcccg tcaggacaat gaattgcgcg agatcggcgg cagtgctgct gagcttgatc
     4441 agatcaaggc ccgctaccca gccatggccg ttgcggctgg tgcaggcttt gctgccgcgg
     4501 ctgctgaaga gaatctggat agcttcagcc tggacgattt ggccctggac gaaccgagcc
     4561 cgaaagccgc cactggcgat caggatgatg cattcgatct gagtctggat gatcttgagt
     4621 ccgaactgga tcgtgatctg caggctgccg gaaacactgc ttcgctggat gatctggata
     4681 gcttgtcact ggatgacgat ctggatttca gctcgccagt tgagcaagcc aataacaaag
     4741 acgacgacct gggttttgac ctgagtctgg ctgatagcgg cgatagtggt cttgatctga
     4801 gcggcgacct ggctgacttc agcctggatc tggatgaacc tgccaagcca gcagcggctg
     4861 ctgaagatga tttcctgctg agcctggatg atgaaccaag tgcagcgccg gctgtggctg
     4921 agtcaagttt tgacctgcca gatgagcaag cgggcggcga ctttgacctg tcggctgatt
     4981 tcgatctgtc gctgaatgat gaggcgcctg cgcaaccatc gagcgacagt ttcgctgctc
     5041 agttggatga agttagcgct gagctggatc tgctatctga tgatctggat cagccagcgg
     5101 atctgggtat gccggcaagc gccgcctcgt ccagtatgga taatgacgat gacttcgact
     5161 tcctctcggg taccgacgag acggcaacca agctcgatct ggctcgcgcc tacatcgaca
     5221 tgggtgatac cgaaggtgct cgcgatatcc tcgatgaagt catcaccgaa ggtaacgaag
     5281 gtcagcaaaa tgaagcgcgt gagctgatcg ctaaattggt ttgatacatg tctgatggtc
     5341 ttactacaac ggcagccgat tcggctgccg ttggcgtttt caagatagcc ctgggtgtcg
     5401 agtacaaagg ttcgcgctat cgcggctttc agcgccagcg tgctggcgtg ccttccattc
     5461 aggaatcatt ggaaaaagcc ttggcgaaag tcgctggcgg cgttcccgtt accctgagct
     5521 gtgccgggcg taccgatgca ctggtgcatg ccagtgccca ggtggtgcat tttaatacac
     5581 cggtcgatcg ctccatgcat gcctgggtca tgggggcgaa tatgaatctg cccggtgata
     5641 tcagcgtgac ctgggccaag tcgatgccag cgcactttga tgcgcgcttc tgcgcccagg
     5701 cgcggcgcta tcgttatgtg atttacaacg accagattcg cccggcgcat atggccgaag
     5761 aagtgacctg gaaccatcgg ccgctggatg tcgagcgcat gcgtcaggcg tctcgcgcat
     5821 ttatcggtac ccatgatttc agtgccttcc gcgcccgcca gtgccaggcc aagtcgccga
     5881 tcaagacggt gcatcacctg gagttactgc agtacgggcg ttttatcgtg ctggatatcc
     5941 gtgctaatgc gttcctgcat catatggtgc gcaatatcgc cggggtgctg atgaccattg
     6001 gaacgggtga gcgaccggtt gagtgggcgc aggaagtgct ggaaacccgc gtgcgtcgca
     6061 ccggtggagt gaccgcgcat ccctatgggc tgtacctggt gcaggtcgat tatcccgaag
     6121 agtttgactt gcccaagcgc tatctgggcc cgcactttct ttccggtttg ccggacctgg
     6181 cggcgagcag ctgaaaaatc atgtgttaaa agctgccgac ttttcgcggc cacgtggctt
     6241 gcgtagaggc gtcaacagac cctagcatcc cgcttcaact gaatttatcg aggtcatcct
     6301 gttgtcagcc gttcgcagca agatttgtgg cattacccgc atagaggatg cattgtcggc
     6361 tgtcgaggcg ggtgcggatg cgattggcct ggtgttttat gccaagagcc cgcgcgccgt
     6421 cacgctgcag caggcgcgct cgattattgc tgccttgccg ccttttgtga ctacggtggg
     6481 cctgtttgtc gatgccagcc gctgcgagtt gggcgagatt ctcgatgcag taccgcttga
     6541 tctgctgcag ttccatggcg atgagagccc ggaagactgt gatggctacc accgacccta
     6601 tatcaaggca ctgcgggtca aacctggcga tgacattgcc gcccaggtgg cgctctacaa
     6661 gaatgccagt ggtgtgttgc tcgataccta tgtgccaggc attccgggtg gcacaggtga
     6721 ggcgtttgat tggtcgctgg tgcctgaagg tttgagtaag ccggttattc tggcgggcgg
     6781 cctgacggct gagaacgttg cacaggccat cgttcaggtg cgtccctatg ccgtagatgt
     6841 cagtggcggc gtagaaatgg ccaaaggcat caaggatgcc gcgaagattc gcgcttttat
     6901 gcaggcggta aaggctgcct aaggtgcgcc aggttgatgt gacgacgctg cgggcttggc
     6961 cgtccataac cctgttgcag gttggataaa cgctgcaggc ggtcgttcga ggtggcgggt
     7021 gagtatgtgc tgatggcagt gcaccggccc ctgaaacgac tgtacccatg aattttcccg
     7081 gttaggcttg gtcgcattag cgcagcccgc ccgatcaaca gctttggaga gtcaagagca
     7141 tgagcaactg gttggtagac aaactgatcc catcgatcat gcgttccgag gtcaaaaaga
     7201 gttcggtgcc cgagggcttg tggcacaagt gcccgtcctg tgattcggtg ctgtacaagc
     7261 ctgaactgga aaagaccctg gatgtttgcc ccaagtgcaa tcaccacatg cgcatcgatg
     7321 cccgctcgcg cctggatatc ttcctggatg ccgagggtcg tgaagagctg ggcgccgacc
     7381 tggagccggt tgaccgcctg aagtttcgcg acagcaagaa gtacaaggat cgcctcaccg
     7441 gtgcgcagaa gcagaccggt gagaaagatg ccctggtagc cattcgcggc accctcgaag
     7501 gcatgccgat tgtgacctgc gcattcgagt tctcctttat gggtggttcg atgggcgcca
     7561 tcgttgggga gcgttttgtc cgcgccgcca acgcggccct ggagcagcgt tgcccgctgg
     7621 tgtgcttctc cgcatccggc ggcgcacgca tgcaggaagc gctgatctcg ctgatgcaga
     7681 tggccaagac ttcggccgtg ctggcgcgtc tgcgtgaaga aggtattccg tttatttccg
     7741 tgctgactga tccagtgtat ggcggcgtgt ctgccagtct ggccatgctc ggtgatgtaa
     7801 ttgtcgccga accccgcgca ttgatcggct ttgccggccc gcgcgtgatc gagcagaccg
     7861 tgcgcgagaa attgccagaa ggcttccagc gcagcgagtt cctgctggag cacggcgcta
     7921 tcgatatgat cattcaccgc gccgaattgc gtccgcgcct ggcgcgtctg ctggctcagt
     7981 ttatggggct gccatcgcct gtagccttgc cggcgactgc atgaccgagc gctcccttgc
     8041 cgattggctg agctatctgg agcagctgca tcccagcgcc attgatatgg ggttggagcg
     8101 ttctcgggta gtgctgcagc gtcttggctt gaccaagctg gcgccgcggg tgattaccgt
     8161 caccggaacc aatggcaagg ggtcgacctg cgcatttatt gcctcgctgc tgcaagggca
     8221 ggggctgaag gtcggtgttt acagctcgcc gcacctgctg cgttacaacg agcgggtgca
     8281 gattgctggc gtcgaagcca ctgatgccat gctctgtgag gccttcgccg tggtcgaagc
     8341 cgcgcgcggt gaaacctcgc tgacctattt cgagatgggc actctggccg ccttttggtt
     8401 attccagcaa gctgggctgg atgctctggt gctggaggtc ggccttggcg gccggctgga
     8461 tgcagtgaat ctgctggatg ccgatatcgc tctggtcacc agtatcggca tcgatcatgc
     8521 cgactggctg ggtgataccc gtgagtcggt cgccttcgag aaggccggca tcttccgcgc
     8581 gggcaagcct gccttgtgtg gcgatcttga gccgccgcag cctttgcttg atcaggttgc
     8641 gctgttcgat gctccattct tcctgcgtgg tcgtgactat gacctgagca tcaatgcaca
     8701 ggattggtcc tggtacggcc tcaatcatca aggcgaagtg ctgagcctgg cacacttgcc
     8761 gctgctcgat ctacccatgg aaaatgctgc gctggccttg caggcttacg cggtgatgca
     8821 attgccctgg cagcctgagg tgatcagtca ggcattgcag cggacgcggg tgaccgggcg
     8881 actcgatcgc agaatgctta attggcaggg taagtcactc acgctgctgc tcgatgttgg
     8941 gcataatccc catgctgccg agtatctcgc tcagcgcctg gctagtcggg cgttgtcagg
     9001 caagcgcctg gccgtgtttg gtttgctggc tgacaaggac ttgcccggtg tggtggcgcc
     9061 tttgctgagt gaagtggaca gttgggcggt ggcggctttg ccgacaagtc gcacgcgcgc
     9121 agccgccgag ttgcaggcgc atctgcataa cagcggggcg caagtgtccg cttatgccag
     9181 tgtgcaggca gcgcttgaag ctcagtgcga gcaggcttcg gatggggatg aagtgctgct
     9241 gtttggatct ttctactgcg tggccgaggc cctggattgg ctggcccgcc aagccaatgg
     9301 gggcgtgcag gatggctttg ctggataagg gattgaagca gcgcatggtc ggtgcgctgg
     9361 tattgctggc gctggcagtg attttcctgc cgatgctgct gtcgcgtgaa gatgagtcgc
     9421 agcgggttca ggttgatgca ccggcaatgc cggcggctcc cgaggctcct gaggtcgaat
     9481 tgcagccggt agccgtgcct gagccgcagt tattgcctga tgagccgatt ggtagcgagg
     9541 ccgcgcaaag cctggagcca agcgagcctg ttgtgagtga gttgccggcc gaacccatca
     9601 ctccagcggc accagcggcc gcgcctgagc ctgtggcaaa accacctcag cctgcgccga
     9661 gtaagctgga tgccaatagc ctgccgatca gttggtcggt gcagctggcc agtctgtcca
     9721 gtagcgatgg cgcgcagaaa ctgcagaaaa ctctgcgcag tcagggctac aacgcctata
     9781 tccgcagtgt cgatggcatg aaccgggtgt ttgtcgggcc gctgatcgag cgtgccgagg
     9841 cggatcgcct gcgtgatcag ctcaatcgtc agcacaaact caatggtttt gtggtgcgtt
     9901 ttcagcccga agccagctga ttaagcggct tggtgtacct cgccaagccc agctgtctgg
     9961 cctgccgcta ggcgggccgg gcatttcagc ggttggcgaa tcctttgctt acgcctcgca
    10021 caggtgctct gctaaaatgc agcgcctttc ccgtcggtaa cctgcatcgt ggcattcacc
    10081 tgggtcgatt gggcgattat cgccgttgtc gccgtttcaa gtttgatcag tctgagccga
    10141 ggcttcgtca aggaagccct gtcattgctt acctggatcg ttgctggcgt ggtcgcctgg
    10201 atgttcggcg gcgcactgtc gcagcacctg gtggatttta tcgagacgcc atcggcgcgc
    10261 gtgattgcag gttgcgcgat tctatttgtg gtcaccctgt tggtcggtgc cttggtcaat
    10321 tacctgattg gtgaattgat ccgggtgacc ggactgtcgg gaaccgatcg ttttctcggc
    10381 atggtctttg gcgcggcgcg cggcggcctg ctggtcgtag tgctggttgg tctgatcagc
    10441 ctggcaccgg ttcagcaaga cagctggtgg caggagtcag cacttgtacc gcattttctg
    10501 atggttgcgg actggtcgaa aaacctgatt ctgggtctgt ccagtgagtg gctggccagc
    10561 ggcgttactg cgccggtgga gttaccgttc aaagaggcac tgcagcagcc aaaactgccg
    10621 taagtgcatt gcgaatcatc gtgtttcatt gagtaggggt tgcgtcacat gtgcggcatt
    10681 gtcggtatcg tcggtaaatc gaacgtcaat caggcgctgt atgacgggct caccgtcctc
    10741 cagcaccgcg gc
//
LOCUS       DASRUM010000077        28247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_700082, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000077 DASRUM010000000
VERSION     DASRUM010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 28247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28247
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700082"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /gene="urtB"
                     /locus_tag="VFF22_05440"
     CDS             complement(<1..888)
                     /gene="urtB"
                     /locus_tag="VFF22_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010175252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HZX16291.1"
                     /translation="MEWLSEFGAIAAMQGFNGLSVFCVLLLMALGLAIIFGQMGVINM
                     AHGEFLTIGAYTTYVVSVLTEQYAPFFGPYYFFLAIVLSFFIAGAIGWLAEWAMISKL
                     YQRPLDTLLATWGLSLVMQQAFRSIFGAREVSATLPEWLMGSWNPTEAIDIPRNGLFV
                     MGLTVLMTGMIFLMLYRSRWGLQVRATVQNRVMSRAVGINTKKVDRMTFALGCGVAGV
                     AGAAFTTIGSTGPTAGSLYIVDTFLVVVFGGASSLLGTIASAFSIAQAQSLMEFFMSG
                     SMAKVFTLSAVILILMLRPQ"
     gene            complement(990..2219)
                     /gene="urtA"
                     /locus_tag="VFF22_05445"
     CDS             complement(990..2219)
                     /gene="urtA"
                     /locus_tag="VFF22_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HZX16292.1"
                     /translation="MDSPTRFLPRRLALGAAALSLLAASVFSQMALADVNTTGLAVTD
                     TEVTVGQLHSATGTMAISETGSIQAERLAIEQINASGGILGRQIKIIQEDGASDWPTF
                     AEKAKKLLVGDKVAAVFGCWTSASRKAVLPVFEKENGLLYYPTFYEGLEQSKNVIYTG
                     QEATQQILAGLDWIAKEKGAKTFYLIGSDYIWPRTSMKIARKHIENVLGGTVVGEEYY
                     PLGNTQFGSLINKVKLKKPDVVFTAVVGGSNVAFYKQMKAAGVTSAKQTLLTLSVTED
                     ELLGIGGENMEGFYASMKYFQSLDNPNNAAFVEAFKAKFGKDSVIGDVTQAAYLGPWL
                     WKAAVEKAGSFDVDKVAAASPGIELTTAPEGYVKVHDNHHLWSKTRIGEVQKDGQFKV
                     VFESDLIEPNPFPKGYQ"
     gene            2501..5899
                     /locus_tag="VFF22_05450"
     CDS             2501..5899
                     /locus_tag="VFF22_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020294151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX16293.1"
                     /translation="MQPTGTQRIVKIRRDYNSWVADETMEDYALRFTPKSFRKWSELR
                     IANTALGAVSFLALEAIGGALALSYGFTNTLWAVLAVALVIFLTGLPISYYAARYGVD
                     MDLLTRGAGFGYIGSTITSLIYASFTFLFFALEAAIMALALELYFNIPLALAYVICSV
                     IVIPLVAYGITLINRLQMWTQPLWLFLLFLPYIFVIGKNPEAISDWTSFAGREGEGGT
                     FNLLYFGAACTVGLALVTQIGEQVDYLRFLPEKTQRNRKRWWAALLMAGPGWIIPGAL
                     KMLAGAFLAFLALQHEIPMERAAEPTQMYLVAFNYVFSSPEWALAAMVLFVIVSQVKI
                     NLTNAYAGSLAWSNFFARVTHSHPGRVVWLVFNVAIALMLMELGVFQAIEEVLGLYAN
                     IAIAWIGAVVADLVINKPLGLSPKHIEFKRAHLYDINPVGVGAMLIASLLSILAHGGL
                     FGQLAQAASPVVALGSAMLFAPLIAWLTGGRYYLARSSDPLLLYPLGVTTTVQCGLCS
                     NAFESEDMAFCPAYSTPICSLCCSLDARCGDACKPRARLSEQLDDFIRWLFPRVSIPR
                     LHSRLAQYLGLLTVLVALLFGALALIYSQVSQGLVAQSAEAQGTLYLAFLKAFLTLSL
                     FAAILAWWVVLTRESRRVAQEESDRQTQLLMQEIEAHRKTDEALQKAKEASEAANAAK
                     SRYVTGLSHELRTPLNSILGYTQILQRDAGMPGQHQDALATIFRSGSHLLSLIDGLLD
                     VAKIEAGKLNLELTEIPFPEFIHQLERMFTPQAEEKGLRFRLKTSGRMPAVVRGDEKR
                     VRQILINLLGNAVRFTDSGEVCLRVSYLRETASFEIIDTGIGIDPEQIERIFQPFERG
                     DLMRQDNGVGLGLTITRMLTSLMGGELSVKSVQDQGTSFQVRLFLSEVRVPQAVVHVE
                     HDIIGYQGPRRLILVVDDHIEHRRVLAGMLEPLGFTIAQAASGQEAIRQVALLHPDLI
                     LMDLSMPQMDGWETSRLIRRNAQSQAPIIVISANAFADDRERSVSADCNDYLAKPVHT
                     PELLERIHKHLDLQWLRRTLQPTAAAPDALVRPSPNDLDALRELGAIGYIRGIQEKLD
                     AIDRLTPASTGFTAPLRALVKSYRLDEFNRQLKEAEHERADPSI"
     gene            5874..6572
                     /locus_tag="VFF22_05455"
     CDS             5874..6572
                     /locus_tag="VFF22_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020294152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX16294.1"
                     /translation="MNALTHPSEHHARAAERGVILIVDDTPDNLALLSDALDEVGYMV
                     LVALDGLSALSRIQRRRPDLILLDAMMPGLDGFETCQRIKADAATADIPVLFMTALTD
                     SEHVVKGFEAGGIDYVTKPINTEEVLARVASHLRTARILQTARAASQAQPLTLNDEPA
                     YATLSARFQLTEREVEVLRWVACGKTNRDIGDILGLSPRTVNKHLEHVYVKLGVETRT
                     AATSVALSAMAERA"
     gene            complement(6701..8731)
                     /locus_tag="VFF22_05460"
     CDS             complement(6701..8731)
                     /locus_tag="VFF22_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004352859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX16295.1"
                     /translation="MKTVLYPAIALMNRLSFGMKFSLISVLFFLPMLVTNFYLVRDSY
                     RQFISTQAALESIDLLGESLTLRRNLEDFVDLTEINAMIGQSGQAGDLEARLVKLQGS
                     IGEALQGMAAVVREPEQIEEFNSKRDQLIAELAAVQAETSLQGKIGLAEKLLGSSQVF
                     IKLVAGQAGLSQDRQGSVRQMIELVTVDTPKVTAVLSEGRAVGAYSLAQGFLNSSAST
                     AFDELLLELEKLHGEYGLNLQASLNSSAQARTELASQAASSLETLKTTVVVFEDKVVV
                     ADSLDTPWAQFYADISGAMEQTYQLNDAVLVFLDKQLQVRLEENRTQMVLLVAALVVV
                     FLLIVYLYGGFYVSIRTTLKKLGQVMNQVAAGDMTVSFKAQSQDELGELGQVFNETVS
                     KIHQLIERVGQTVVEVERQADRVQQVSGESNQAVAGQRGQIDQIATAMNQMSATAQEV
                     AHSAAAAVGSAQSVNDETVSGRALVESQVGSIQRLAGEIDQSVAVINKLASDSASISQ
                     VLDVIKGIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRNLAKRTQQSTEEIESMI
                     AKLQSGVGAAVKAMSASHKMADTTVSESGKVQQALENILGAVGMIVDQNQQIATAAEE
                     QTAVAHDIDQNIVEISQAGERTAEGASQTEQASRELSGLVARLKQLIGAFRV"
     gene            8951..9844
                     /locus_tag="VFF22_05465"
     CDS             8951..9844
                     /locus_tag="VFF22_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZX16296.1"
                     /translation="MRTPLKLGIDLGGSKVEIIALENGQQVLRRRAPTPQGNYQKTLT
                     LIASLVEDCERELGSRGSVGVGIPGTRSPDHGRIKNANSQCLIGEDLQADLEQRLQRP
                     VRLANDADCFALSEACDGAGEGAVSVFGVILGTGVGGGLVVRQQLLSGPNAIAGEWGH
                     NPLPWRRNEDGPSRHCYCGQEDCIETFLSGPGWAARSNLGLDASALVSAAEAGDSAAE
                     QAFARYCDQLARGLASVINLMDPHVIVLGGGMSNSTALYTQVAPLLPRYVFSDQVNTR
                     LLQAKHGDSSGVRGAAWLWNE"
     gene            complement(9851..10687)
                     /gene="ampE"
                     /locus_tag="VFF22_05470"
     CDS             complement(9851..10687)
                     /gene="ampE"
                     /locus_tag="VFF22_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory signaling modulator protein AmpE"
                     /protein_id="HZX16297.1"
                     /translation="MNFLVLLLVLWVEKFSSWRKPIQHDGPWLRLLTATESKPQWAGR
                     PWLALALLVLLPLLALGLILLVLEPVAYGWLALPVHLLVVIYSLGRGDLLAALGPFRD
                     SWRREDAEAAYLVARRDLALEADEEGELLQRVQGHLVWQAYQSFFAVIFWYGLLGPLA
                     ALAYRLVALVSEHATLSTLREAAVQVRHGFDWLPVRVLAASFALVGNFAGVSRALLHE
                     LLSWDISAAQLAIITARAAGETPEPVIGEVGVSSLDGLWQLLVRAAVFWYAVFAVWTL
                     LL"
     gene            complement(10684..11247)
                     /gene="ampD"
                     /locus_tag="VFF22_05475"
     CDS             complement(10684..11247)
                     /gene="ampD"
                     /locus_tag="VFF22_05475"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HZX16298.1"
                     /translation="MQLDPSSGWCDGIQRCPSPNFNQRPLGEVSLLVIHNISLPPGQF
                     GTGKVQAFFQNRLDADEHPYFASIASLQVSAHFFIERDGAVTQFVSCNERAWHAGVSR
                     FAERGNCNDFSLGIELEGTDDLPFTAAQYAALIELIQQLQAAYPAITLERICGHSDVA
                     PGRKTDPGPAFDWQRVRLALQQDKELI"
     gene            complement(11389..13695)
                     /locus_tag="VFF22_05480"
     CDS             complement(11389..13695)
                     /locus_tag="VFF22_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 domain-containing protein"
                     /protein_id="HZX16299.1"
                     /translation="MKTDANVVHLSKVTPEQSPTSPVGRLPVALTNVRDKAAQQLKTA
                     LQALFDNADDTLFEMADRATSNTEQNAFFEAMRDLRLKRKNIERGFLQKVFESFATLN
                     QYEIGKPAPLDAVSFDTLSLVQNDALEESVALDSMVAKVVSRDGTALSHLTTRFNAMV
                     SKKLDDKSNPVGPTCLCEFFLDACSSLGVEIRVKLIILKLFEKYVLSGLDQLYAEANQ
                     ALIAGGVLPELKSAPARRNPSRASASASGTRQDASPELSTEFADEGVQEVFGALQELL
                     SQARGSVIPRRNRPADAMPISSNDLMRLLSHMQQRAPVQASDDFDLREQLEGLLTRAS
                     AKAGKARVVGEVDEDVINLVSMLFEFILDDRTLPDSLKALIGRLQIPMLKVAVIDKTF
                     FSRGSHPARRLLNEIASAALGWGEQDDAQRDSLYQKIEQVVQRLLNDFVDDPVIFSEL
                     LAEFLAFTGDERRRSELLEQRTRDAEEGSAKAALARREVEQALNERLLGKTLPEVVVR
                     LLQEAWSKVLMLTCLKHGVESAEWQAALATMDDLVWSVELHEDPEARMRLLEMVPGLL
                     KALREGMASAAFDPFSTGEFFSQLEALHVQAFQRFKRSLPEENPQDDMPALDDEQRAA
                     LVEAGIDLPLLELPAADAVEEPAMVEVVEEIILLAPGELRAAEPENSLPDDDESLLQV
                     DNLRVGSWVEFQEDEEHKLRCKLAAVIKPTGKYIFVNRTGMKVLEKTRMGLAVEFRRS
                     AIRLLDDALLFDRALESVIGNLRKLKGA"
     gene            14050..14898
                     /gene="nadC"
                     /locus_tag="VFF22_05485"
     CDS             14050..14898
                     /gene="nadC"
                     /locus_tag="VFF22_05485"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HZX16300.1"
                     /translation="MPNLILADLANEIEANVRRALAEDIGSGDITAQLIPAERLAHAK
                     VITRESAVICGTAWVDAVFRQLDPRVAVHWHIQDGQRAEANQVLFELEGPARALLSGE
                     RSALNFLQTLSAVATRCQHYADLVQGTAVKLLDTRKTLPGLRLAQKYAVTQGDCHNHR
                     IGLYDAFLIKENHIAACGGIAQAVTAAHKIAPGKPVEVEVESLEELRQALDAGADIIM
                     LDELSLDEMREAVALTAGRAKLEASGGVNESTLREIAETGVDYVSIGALTKDVKAVDL
                     SMRLSL"
     gene            complement(15007..15906)
                     /locus_tag="VFF22_05490"
     CDS             complement(15007..15906)
                     /locus_tag="VFF22_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX16301.1"
                     /translation="MSAVLEFNGVSHAFRSKSKEVQALRDVSFSLTEGEVFGFVGPNG
                     AGKSTTIKVMLDIINDYQGEVRIYGVDARRAEARQPLAFVPESPALYEQFTPLEILRM
                     GLSMYGIKRKDADAWCMQWLERFSVVQNAKRRIRELSKGNVQRVALAHAMVVQPKLLV
                     LDEPLSGLDPVGRKDVVGILTEYKQQGGAIFFTSHVLHDVERIADRFGFINKGELLTV
                     RSPRELAAERVDQMLVRFNAEQPMIDGCKQLREMEFECELSQADLPAFIAQLNNVGGH
                     LLTVKPAVSLETVFFKILEQAAR"
     gene            complement(15906..16748)
                     /locus_tag="VFF22_05495"
     CDS             complement(15906..16748)
                     /locus_tag="VFF22_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16302.1"
                     /translation="MSMSRHRYRSLLLALLGFGVFSVAVQWRAEVPVPVAPIIEGRVV
                     IAAPVLAALFGGDRFLAASLETMRLASTGVDLGQANTGYLVRAQQVVAELNPCHEDNY
                     YLANGLLTWGGADREGTEVLRRAMQCRFWDELPAFFYGFNQAFFNKDIAEASRALELA
                     AQRSPVNAAGFRKMVVMLQAESFADEKLALDYLIQQRDAASDPKLRDMLGQRAVRLQG
                     LVALREAQRRFEAESGALVSLDQLVEHGLLPALPDDPLRLGYELREGEIILKKMKIAG
                     LEGY"
     gene            complement(16745..17518)
                     /locus_tag="VFF22_05500"
     CDS             complement(16745..17518)
                     /locus_tag="VFF22_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX16303.1"
                     /translation="MAWRARYLWLSGCSLLMLALAAMLASQFSGRQPATVALDVGLSV
                     MRLLLPMVLVLMIQELLSREFDRRYFLNSLTYPRPRHHLLLGRFLAVAVLTLGLLMAM
                     ALLLALLVELVSQGYVQGTPVAMGRHYLISIVFVGVDLLLLTALACLLAVVASTPSFV
                     LVGTFGFMLAARSFAAIVELLGRNASVVGDAESYRAGVGLLGYLLPDLGALDVRMIAL
                     YGSMKFLPADWFWLLLSSLAYLVALLALAVWALQRKRFA"
     gene            complement(17777..18292)
                     /locus_tag="VFF22_05505"
     CDS             complement(17777..18292)
                     /locus_tag="VFF22_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16304.1"
                     /translation="MKKILFASLAASLVFVAQAHAAGTGTLPASGTVTNVQCTWITSN
                     VSATLSAGVVAAYDCNSNANNTIFIGTASQRGTTKNRTIACAVTSSDVGADTTTCNNS
                     TCASTVPSSAGGATCTGNFQATGNTLFTASSDGGSVTAGSVGTTCDADPAQAGACANS
                     VLTAVSAAAAQ"
     gene            complement(18338..18523)
                     /locus_tag="VFF22_05510"
     CDS             complement(18338..18523)
                     /locus_tag="VFF22_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZX16305.1"
                     /translation="MKSLKKQKGFTLIELMIVVAIIGILAAIAIPSFNEYRQKASDGT
                     ANSDARNFITLMVSMAN"
     gene            18757..20460
                     /gene="pilB"
                     /locus_tag="VFF22_05515"
     CDS             18757..20460
                     /gene="pilB"
                     /locus_tag="VFF22_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HZX16306.1"
                     /translation="MSEAPNLTGLARQLVNAELLSESTASQAQAQALRNKIPLVSYLV
                     QNKLATSKAVAEVAAEQFGVPFCDLNAIEKESQPRDLISEKLVRQHRVIPLFRRGNKL
                     FVGVSDPTNHQAVTDVQFSTGLTTEPLLVEDDKLTDAIEKFFDTANSGMEELGDVDLD
                     GVDVEAVNDDKQDGNDGNSADDAPVVRFVHKMLLDAIKKGSSDLHFEPYEKTYRVRFR
                     TDGILQEIARPPIQLAPRISARLKVMAGLDISERRKPQDGRIKMRISKNKSIDFRMST
                     CPTLWGEKIVMRILDSASAQMGIDALGYEESQKDLYMTALKQPQGMILVTGPTGSGKT
                     VSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFTAALKAFLRQDPDI
                     IMVGEIRDLDTASIAVKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLI
                     IAQRLARKLCSHCKKEVKIPREALLEEGFPTDKVDTLKLYGPVGCENCNGGYKGRVGI
                     YEVVKNTPALQRIIMEEGNSIDISVQMRKDGFNDLRTSALLKAMQGITSLEEVNRVTK
                     D"
     gene            20464..21684
                     /locus_tag="VFF22_05520"
     CDS             20464..21684
                     /locus_tag="VFF22_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZX16307.1"
                     /translation="MAAKAPSISTFAWEGTDKKGAKIKGEINGQNPALVKAQLRKQGI
                     NPLKVKKKPKPLFSAGKKIKPMDIALFTRQMATMMKAGVPLLQSFDIIGEGFDNPKMR
                     TLINDVKQEVAAGNSFAASLRTRPQYFDDLYCNLVDSGEQSGALDTLLDRIATYKEKT
                     EALKAKIKKAMTYPISVLVVAVIVTAILLIKVVPQFESVFAGFGAELPAFTQVVVGMS
                     RGLQEWWYLFLLAFFVIGFTFKEAHKRSEKFRDFLDRTLLKAPIIGDILYKSVVARFA
                     RTLSTTFSAGVPLVEGLQSVEGAAGNVVFRQAVAKIRNDVSSGTQLNFSMRTTGVFPS
                     MAIQMTAIGEESGSLDEMLDKTAEYYEAEVDNAVDNLTTLMEPLIMSVLGVLVGGLII
                     AMYLPIFQLGSVVS"
     gene            21688..22554
                     /locus_tag="VFF22_05525"
     CDS             21688..22554
                     /locus_tag="VFF22_05525"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HZX16308.1"
                     /translation="MIPDLLAGNLPAFVCVGLLLGLLIGSFLNVVVYRLPIMMQRGWR
                     EQAREILELPAEPQTATFNLVLPNSSCPHCAHEIKPWENIPVISYLFLRGKCSSCKAP
                     ISLSYPLVELTCGLLSAFIAWHFGFTWQAGAMLLLTWGLLAMSLIDVDHQLLPDSLVL
                     PLLWFGLIANSFGLFTSLEDALWGAIAGYLSLWSVYWLFKLVTGKEGMGYGDFKLLAM
                     LGAWGGWQVLPLTILLSSLVGAVLGLIMLRLRNAETSTPIPFGPYLAIAGWIALLWGE
                     QITSSYLQIAGF"
     gene            22839..23453
                     /gene="coaE"
                     /locus_tag="VFF22_05530"
     CDS             22839..23453
                     /gene="coaE"
                     /locus_tag="VFF22_05530"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253219.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HZX16309.1"
                     /translation="MTTKPWILGLTGGIGSGKSAVAQHFIDLGVHLVDADHAARWVVE
                     PGKPALAKITEHFGSDVLQADGQLDRTALRSRIFADADERRWLEALLHPLISQEISQY
                     LARAESPYAILVSPLLVESGQHKLTQRILVVDAPEQLQMQRTMARDQSSNEQVQAILK
                     AQASREERLRHAHDVLLNDQDLPWLRAEVERLHTFYLSLRGGQA"
     gene            23450..23650
                     /gene="yacG"
                     /locus_tag="VFF22_05535"
     CDS             23450..23650
                     /gene="yacG"
                     /locus_tag="VFF22_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HZX16310.1"
                     /translation="MSTPTLVACPTCSAPVEWGPQSPSRPFCSERCKLIDLGAWASEE
                     HAIAGNPLEDDVFSGELPPRGH"
     gene            complement(23908..24123)
                     /locus_tag="VFF22_05540"
     CDS             complement(23908..24123)
                     /locus_tag="VFF22_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020750398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16311.1"
                     /translation="MGVHQWARQAIEQSLRGVEAQGFDQAMALRALLSAVIERSRAGR
                     STADLAAELQFLADNLDDERDYSFMRP"
     gene            complement(24368..25045)
                     /locus_tag="VFF22_05545"
     CDS             complement(24368..25045)
                     /locus_tag="VFF22_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012722155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HZX16312.1"
                     /translation="MIAAHLLSSTSQILGWLIYLPLLCWAIWRAPWVELCSDLRRQHL
                     LGGTALALFVLWLVRRDFDSGLSYHFIGMTAVTLLLDWPLAMLVGLIAQLGLLALGKQ
                     DFAALGVNGVLLVVIPVWVTEACALWVEKKQPRNLFVYIFCSGFFAAALAALLCIAAA
                     FTVLWVDGLFPMPSWLEDFIGYLWLVIFPEAFINGMLVTALVVFYPDWLETFNRTRYL
                     AAPWKDG"
     gene            25341..25970
                     /locus_tag="VFF22_05550"
     CDS             25341..25970
                     /locus_tag="VFF22_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1780 domain-containing protein"
                     /protein_id="HZX16313.1"
                     /translation="MDDSDYLRLLTHQAEQANAFLSNARKWERERWVCQRLLQALNVS
                     HRLDEFSAANHEPPDVLFREASFEVFFVLDEGRRLNDEWRAELERRRSAFSLSQLVRR
                     EAKPRRIAAAELQQRLTPTLRKKAHNYKERGLDLGELDLLAYVNLKRAVPDFNSHFPP
                     PTEFLRQGWRSLSLVGPTFARVLFAHPDAPDFLRNNLGRSVLFDVGISL"
     gene            25967..26461
                     /locus_tag="VFF22_05555"
     CDS             25967..26461
                     /locus_tag="VFF22_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017708524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HZX16314.1"
                     /translation="MSPLHELLAAVPQQGCVRWIGVRPASRAEMIELDAVEARREAGL
                     TGDHSRPGPRNARQVTLIQWEHVAVISALMGRPAEQPISAPELRRNIAISGINLFSLK
                     GRRFRLGQAILETTGWCQPCAKLEERLGLGTFQAMRGHGGITARVLQGGIIRLNDALR
                     VEPL"
     gene            26544..26717
                     /locus_tag="VFF22_05560"
     CDS             26544..26717
                     /locus_tag="VFF22_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004577274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3094 family protein"
                     /protein_id="HZX16315.1"
                     /translation="MSSRLNPDDQKRVEQYLSAPQHQVERQPFKVWRLLLIIVTITIG
                     LGLLSRLLSRLVL"
     gene            26781..28082
                     /locus_tag="VFF22_05565"
     CDS             26781..28082
                     /locus_tag="VFF22_05565"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZX16316.1"
                     /translation="MTHQIVIVGGGAGGLELATRLGRTPGKRGQARITLVDANLTHIW
                     KPLLHEVAAGSLNSSGDELNYVAQAKWNHFEFQLGRMCGLNRAEKRISLAATLDDDGQ
                     VLMPARELSYDSLVIAVGSTTNDFGTAGAAEHCLFLDTREQAERFHRQLLSHYLRAHA
                     SQSAATELINIAIVGAGATGVELAAELHHAAHELAAYGLNRISPENMRITLIEAGPRV
                     LPALPERISRPVHKTLEKLGVTVLTGAAVSQVTREALHTEQGQVIPATLKVWAAGIRA
                     PDFLKELDGLESNRINQLHVRLTLQTTLDDNIFAFGDCAACPQPGSEGRNVPPRAQAA
                     HQQASLLAKSLKLRIEGKTLPEYRYRDYGSLISLSTFSAVGNLMGNLTGSVMLECWLA
                     RMFYVSLYRMHQIALYGITRTLLMMLGDRIGRSTEPRLKLH"
BASE COUNT         6295 a         8842 c         7771 g         5339 t
ORIGIN      
        1 ttgcggacgc agcatcagaa tcaggatcac cgcagacagg gtgaacacct tggccatcga
       61 accgctcata aagaactcca tcagcgactg cgcctgggca atgctgaagg ccgaggcgat
      121 ggtgccgagc aggctcgacg cgccaccgaa gaccaccacc agaaaggtgt cgacgatgta
      181 cagggaaccg gcggtcggcc cggtcgagcc gatggtggta aaggccgcgc cggccacccc
      241 ggctacgccg caaccaaggg caaaggtcat gcggtcgact ttcttggtat tgatccccac
      301 ggcacggctc atcacccggt tctgcaccgt ggcgcgcacc tgcaagcccc agcgcgagcg
      361 gtagagcatg aggaagatca tgccagtcat cagcacggtc aggcccatga cgaacaggcc
      421 gttacgcgga atatcgatgg cctcggtggg gttccatgag cccatcagcc attccggcaa
      481 ggtggcgctg acctcacgcg caccgaagat cgagcggaag gcctgctgca tcaccagcga
      541 caacccccag gtggccagca aggtgtccag cgggcgctgg tagagtttgc tgatcatcgc
      601 ccactcggcg agccagccaa tcgcaccggc gataaaaaac gacagcacga tggcgagaaa
      661 aaaatagtac gggccgaaaa acggcgcgta ttgctcggta agtaccgaga ccacgtaggt
      721 ggtgtaggca ccgatggtga ggaactcacc gtgggccatg ttgatcacgc ccatctggcc
      781 gaagatgatc gccagcccca gggccatcag cagcaggaca cagaaaacgg acaagccgtt
      841 gaagccctgc atggccgcga tggcaccaaa ttccgatagc cattccatga tgatggtctc
      901 cagacggaaa gaaaaaaccc tgtaggagcc ggcttgccgg cgatccattt atcgccagca
      961 atccggctcc tacagatact cgacagcgct tattggtagc ctttcgggaa tgggttcggc
     1021 tcgatcaggt cggactcaaa caccaccttg aactggccat ccttctgcac ttcaccgatg
     1081 cgggtcttgc tccacaggtg atggttgtcg tgcactttca cgtagccttc tggcgcggtg
     1141 gtcagctcga tgccaggtga agctgctgcc accttatcga cgtcaaagct gccggctttt
     1201 tcgaccgcag ccttccacag ccatgggccg aggtaggccg cctgagtcac gtcaccgatc
     1261 accgaatcct tgccgaactt agccttgaag gcttcgacga aggcggcgtt gttcgggttg
     1321 tcgaggctct ggaagtactt catggaggcg tagaaacctt ccatgttctc gccgccaatg
     1381 cccagcagct cgtcctcggt taccgacagg gtcagcaggg tctgcttggc gctggtgacg
     1441 ccggcggctt tcatctgctt gtagaaggcc acgttggagc cgccgaccac cgcagtgaag
     1501 accacgtcag gcttcttcag cttgaccttg ttgatcagcg aaccgaactg ggtgttgccc
     1561 agcgggtagt actcctcgcc gaccacggtg ccgccgagca cattttcgat gtgcttacgg
     1621 gcgatcttca tcgaggtgcg cggccagatg tagtccgaac cgatcaggta gaaggtcttg
     1681 gcgcccttct ctttggcgat ccagtcgagg ccggcaagga tctgctgggt ggcctcctgg
     1741 ccggtgtaga taacgttctt cgactgttcc aggccctcat agaaggttgg gtagtagagc
     1801 aggccgtttt ccttctcaaa caccggcagc acagctttgc gcgaggccga ggtccagcag
     1861 ccgaacaccg cagcaacctt gtcgccgacc agcagcttct tggctttttc cgcgaaggtc
     1921 ggccagtcag aggcaccgtc ttcctgaatg atcttgatct gccggccgag gatgccgccg
     1981 ctggcgttga tctgctcaat ggccagacgc tcggcctgga tcgaaccggt ttcggaaatg
     2041 gccatggtgc cggtggccga gtgcaactgg ccgaccgtca cttcggtgtc ggtcacggcc
     2101 aggccggtgg tgttgacgtc ggccagggcc atttggctga acacactggc cgccagcagc
     2161 gagagtgccg cagcacccaa cgctagccga cgagggagaa agcgagtcgg tgaatccatg
     2221 gtcatgctcc tctgggtggt tttcctccct ggctcgcctt gcgggtacca gttcggttcg
     2281 ccaccagcat gcgtttgcca ccagcgctgg cggtatacgc agcatgacgt aacggcatac
     2341 gtcatttgac gtactgccca acgccccagg ctggtgcaag ctcacgcccg agcttgcagg
     2401 cagacagaag ctcgcgcgcg catggacggc gactgcagac gcagggcatg gatgttgcag
     2461 attcaaacaa gcgtattaac gtcgtcccag gagcgtcctt gtgcaaccga ccggcaccca
     2521 gcgcatcgtc aagatccgcc gcgactacaa cagctgggtc gccgacgaga ccatggaaga
     2581 ctacgccctg cgtttcaccc ccaagtcgtt ccgcaagtgg tcggagctac gcatcgccaa
     2641 caccgccctg ggcgcggtat cgtttctcgc cctggaagcc atcggcggcg ccctggccct
     2701 gagctacggt tttaccaaca cgctgtgggc cgtgcttgcg gtggcgttgg tgatctttct
     2761 taccggcctg ccgatcagct attacgcggc gcgctatggc gtggacatgg acctactgac
     2821 ccgtggcgcc ggcttcggct atatcggctc aaccatcacc tcgctgatct acgccagctt
     2881 caccttcctg ttcttcgccc ttgaggcggc gatcatggcc ctggcgctgg agctgtactt
     2941 caatatcccg ctggcgctgg cctacgtgat ctgctcggtg atcgtcatcc cgctggtggc
     3001 ctacggcatc accctgatca accgcctgca gatgtggacc cagccgctgt ggctgtttct
     3061 gctgttcctg ccctacatct ttgtcatcgg caaaaacccc gaagcgatca gcgactggac
     3121 cagcttcgcc ggccgagagg gcgaaggcgg caccttcaac ctgctgtatt tcggtgcggc
     3181 ctgcaccgta ggcctggcgt tggtcacgca gattggcgaa caggtcgact acctgcgctt
     3241 tctgccggaa aagacccagc gcaaccgcaa gcgctggtgg gccgcactgc tgatggcagg
     3301 gcccggctgg atcatccccg gagcgctgaa aatgctcgct ggggccttcc tggcctttct
     3361 cgccctgcaa cacgaaatcc ccatggaacg cgctgccgag ccgacgcaga tgtacctggt
     3421 ggcgttcaac tatgtgttca gctcgccgga gtgggcgctg gcggccatgg tgctgttcgt
     3481 tatcgtgtcg caggtgaaga tcaacctgac caacgcctat gccggctccc tggcctggtc
     3541 gaacttcttc gcccgcgtca cccacagcca tccggggcgc gttgtgtggc tggtgttcaa
     3601 cgtcgccatc gcgctgatgc tgatggagct gggggtgttc caggccatcg aggaagtgct
     3661 cggcctgtac gccaatatcg ccatcgcctg gatcggcgca gtggtggcgg acctagtgat
     3721 caacaagccg ttgggcctgt cgcccaagca catcgaattc aagcgtgcgc acctttacga
     3781 catcaacccg gtgggcgtcg gcgccatgct gatcgcctcc ttgctgtcga tcctggccca
     3841 cggcggcttg ttcggtcagc tggcgcaggc cgcttcgccg gtcgtcgcac tgggctcggc
     3901 gatgctgttc gctccgctga tcgcctggct gaccggcggg cgctactacc tcgcacgcag
     3961 cagcgatcca ctgttgctct acccgcttgg cgtgaccacc acggtgcaat gcggactgtg
     4021 cagcaacgcc tttgaaagcg aagacatggc cttctgcccg gcctacagca cgccgatctg
     4081 ctcgctgtgc tgctcactcg acgcgcgctg cggagatgcc tgcaagccgc gggcgcggtt
     4141 gtctgagcag ctcgacgatt ttattcgctg gctgttccca cgggtgtcga tcccgcgcct
     4201 gcacagccgc ctggcgcagt acctgggctt gctaacggtg ttggtagcgc tgctgttcgg
     4261 cgccctggcg ctgatctaca gccaggtgtc tcaggggctg gtggcgcaat ccgcagaggc
     4321 gcagggaacc ctctatctgg cgtttctcaa agcctttctg accctgtcgc tatttgccgc
     4381 gatcctcgcc tggtgggtgg tgctgacccg cgaaagccgc cgcgtagcgc aagaggaatc
     4441 cgatcgtcaa acccaactgc tgatgcagga gatcgaagcc caccgcaaaa ccgacgaagc
     4501 cctgcagaaa gccaaggaag ccagcgaagc cgccaatgcg gcgaaaagcc gctacgtcac
     4561 cgggctgtcc cacgagctgc gtacgccgct caacagcatc ctcggctata cgcagatcct
     4621 gcagcgcgat gccggtatgc cggggcagca tcaggacgcc ctggccacca ttttccgcag
     4681 cggctcacac ctgctgtcac tgatcgacgg cctgctcgac gtagccaaga tcgaggccgg
     4741 caagctcaac ctggaactga cggaaatccc cttccccgaa tttatccacc agctcgaacg
     4801 catgttcacc ccgcaagccg aagagaaagg cctgcgcttt cgcctgaaaa ccagcgggcg
     4861 catgccggcg gtggtgcgcg gtgatgaaaa gcgcgtacgg cagatcctga tcaacctgct
     4921 cggcaatgca gtgcgtttca ccgacagcgg cgaggtctgc ctgcgcgtca gctacctgcg
     4981 cgaaaccgcc agtttcgaga ttatcgacac cggtatcggc atcgatcccg agcagatcga
     5041 gcgcatcttc cagccgttcg agcgtggcga cctgatgcgc caggacaacg gcgtcggcct
     5101 cggcctgacc atcacccgca tgctcacctc gctgatgggc ggcgagctgt cagtcaaaag
     5161 cgtgcaagac cagggcacca gtttccaggt gcggctgttc ctttccgaag tgcgcgtgcc
     5221 ccaggcggtg gtgcatgtgg agcacgacat cattggttat caaggcccgc gtcggctgat
     5281 tctggtggtg gacgaccata tcgaacaccg ccgtgtactg gcgggcatgc tcgaaccctt
     5341 gggcttcacc atcgcccaag cagccagtgg ccaggaggcc atacgccagg tcgccctgct
     5401 gcaccccgac ctgattctga tggacctgtc gatgccacag atggatggct gggaaaccag
     5461 ccgactgatt cgccgcaatg cacagagcca ggcaccgatc atcgtgatct cggccaatgc
     5521 ctttgccgat gaccgcgagc gcagcgtaag cgccgattgc aatgactacc tagccaaacc
     5581 ggtgcacacc cccgagttac tcgaacgcat ccacaagcac ctcgacctgc agtggctacg
     5641 gcgcaccctg caacccacgg cagcagcacc agacgctcta gtgcgcccta gccctaatga
     5701 tctcgacgca ctgcgcgagc tcggggccat cggttatatc cgcggcattc aggagaaact
     5761 cgacgccatt gatcggctga cacccgccag caccggcttc accgcgccct tgcgggccct
     5821 ggtgaaaagc tatcgcctgg acgaattcaa ccgccaactc aaggaggccg agcatgaacg
     5881 cgctgaccca tccatctgaa caccacgcca gggccgccga gcgtggggtg atcctgatcg
     5941 tcgacgacac gccggacaac ctggccctgc tctccgacgc cctcgatgaa gtcggctaca
     6001 tggtgctggt ggcgctggat ggcctgagcg cgctgagccg tatccagcgt cggcgccccg
     6061 acctgattct gctcgacgcc atgatgccgg gactggacgg cttcgagacc tgccagcgga
     6121 tcaaggccga cgccgccacc gcagatattc cggtgttgtt tatgaccgcg ctgaccgaca
     6181 gtgaacacgt ggtaaaaggc ttcgaggctg gcggcatcga ctacgtaacc aagccgatca
     6241 acaccgaaga agtgctcgcc cgtgtggcct cacacctacg caccgcgcgc atcctgcaaa
     6301 cggcgcgcgc cgccagccag gcgcaaccgc taacactgaa cgatgagccc gcctatgcca
     6361 cgctctcggc gcgcttccag ctcaccgaac gtgaagtgga agtgctgcgc tgggtggcct
     6421 gcggcaagac caaccgcgac atcggcgaca ttcttggcct cagcccgcgc acagtgaaca
     6481 aacacttgga gcacgtctac gtaaaactcg gcgtggaaac ccgcacagcc gccacctcag
     6541 tggccctctc agcaatggcc gagcgcgcct gaacaggctg actatcgaga ataaaaaggt
     6601 tacatgttgt gaggcgataa gtcgtaggtt ggcgttgagc gcagcgaagc ccaacaggca
     6661 gtggcatacc gcgttgagct ttgttcacac gagctgctga tcagacgcgg aacgcaccga
     6721 taagctgctt caagcgcgcc accaaaccgg acagctcccg gctagcctgt tcggtctggc
     6781 tggcaccttc ggcggtacgt tcgccagcct ggctgatttc gacgatattc tggtcgatat
     6841 cgtgggccac agccgtctgc tcttcggcgg cagtagcgat ctgttgattc tgatcgacga
     6901 tcatgcccac cgcgccgagg atattctcca gcgcctgttg caccttgccc gactcactga
     6961 cagtggtatc agccattttg tggctggcgc tcatggcctt gaccgccgcg cccacgccac
     7021 tttgcagctt ggcgatcatc gactcgattt cctcggtgga ctgctgggtg cgcttggcca
     7081 ggttgcgcac ttcatcggcc accacggcaa aaccgcgacc ctgctcaccg gcccgcgcgg
     7141 cctcaattgc ggcgttgagc gcaagcaaat tggtctgttc ggcaatgccc ttgatcacgt
     7201 ccagcacttg gcttattgaa gcgctgtcac tggccagttt gttgatcacc gccaccgact
     7261 ggtcgatctc accggccaac cgctggatgc tacccacctg ggactcaacc agcgcgcgac
     7321 cgctgaccgt ttcatcattc acgctctgtg cactgcccac tgccgcggcg gcgctgtgcg
     7381 ccacctcctg ggcagtggcg gacatctgat tcatcgcagt ggcgatctga tcaatctgcc
     7441 cacgctggcc ggcaaccgcc tggttgcttt cgccggagac ctgctgcacg cggtctgcct
     7501 gacgttcgac ctcaaccacg gtctggccca cgcgctcaat caactggtgg atcttgctca
     7561 ccgtctcatt gaacacctgg cccagctcac ccagttcgtc ctggctctgc gccttgaagc
     7621 tgacggtcat atcgccagcg gccacctggt tcatcacctg gccgagcttt ttcagagtgg
     7681 tgcggatcga cacatagaag ccgccgtaca ggtaaacgat cagcaggaac accaccacca
     7741 gggcagcgac cagcagcacc atctgcgtac ggttttcctc caggcgcacc tgcaactgct
     7801 tgtcgaggaa taccagcacc gcatcattga gctggtaggt ctgctccatg gcgccgctga
     7861 tatccgcgta gaactgcgcc caaggtgtgt ccagcgagtc ggcgacaacc accttgtcct
     7921 caaacaccac cacagtggtt ttcagggttt ccaggctgct tgcggcctga ctggccaact
     7981 ccgtgcgggc ctgcgcactg ctgttgagcg atgcctgcag attcaggccg tactcgccat
     8041 gtagcttttc cagctccagc aacagttcat caaatgcggt gctggccgat gaattgagaa
     8101 agccctgggc cagcgagtag gcgcctaccg cacgaccttc gctaagaacg gcggtgactt
     8161 tgggcgtatc cacggtgacc agttcgatca tctgccgtac ggagccctgg cgatcctgac
     8221 tcaagccagc ttgaccagcg accagtttga taaatacctg agagctgccc agcagttttt
     8281 cggccaggcc aatcttgccc tgtagcgagg tctcggcctg cacagcagcc agctcggcaa
     8341 tcagctgatc acgcttgctg ttgaattcct cgatctgctc aggctcacgc accacagcgg
     8401 ccatgccctg caacgcctcg cctatgctgc cttgcagctt gaccaggcgc gcttcaagat
     8461 cacccgcctg accggactga ccgatcatcg cgttgatctc agtgaggtcg acaaaatctt
     8521 cgagattacg ccgcagcgtc aggctttccc cgagcagatc aatgctctcg agagccgcct
     8581 gggtactgat gaactgccgg taggagtcac gcacaagata gaagttggtg accagcatgg
     8641 gcagaaagaa cagcacactg atcaggctga acttcatacc aaagctgagg cgattcatta
     8701 gcgcgatggc cggatacagc acggtcttca caggacgtct ccagttctaa ttattgtcgt
     8761 gtaaggtaca aagcggccgt gcagaatctc aaaacggtgg cttgtatgtc ccttgatgct
     8821 cgacctgcgg catttgcatc agctgagtaa atacgggcag atttatgtga tcaatatcac
     8881 aaagtaacgg tcgagcatgg cgtcatgtat acctcttatc gaccccaacc tctgtaactt
     8941 aaggttaatg atgcgcacac cactgaagct cggcattgat ctgggtggca gcaaagtcga
     9001 aataatcgca ctggaaaacg gccagcaagt cttgcgccgc cgcgccccaa caccgcaggg
     9061 caattaccag aaaaccctca ccctgattgc cagcttagtt gaagattgcg agcgcgaact
     9121 aggcagccgc ggcagtgtcg gtgtcggcat acccggcacc cgctcccccg accatggacg
     9181 gatcaagaat gccaactcgc agtgcctgat cggcgaggac ctgcaggccg accttgagca
     9241 gcgcctacag cgcccggtgc gactggccaa tgacgccgac tgctttgccc tgtcagaagc
     9301 ctgcgatggc gcgggcgaag gtgccgtaag tgttttcggg gtgattcttg gcaccggcgt
     9361 cggtggcggc ctggtcgtgc gccagcaact gcttagcggc ccgaatgcca tcgccggcga
     9421 atggggccac aaccccctac cctggcggcg caacgaagac ggcccgagcc ggcactgcta
     9481 ttgcggccag gaagactgca ttgaaacctt tctcagcggt ccgggctggg cggcgcgtag
     9541 caatcttggc ctggatgcaa gcgccttggt gagcgctgca gaagcgggtg attctgcagc
     9601 ggagcaggct tttgcccgct actgcgacca gctggcgcgc ggcctggcgt cagtgatcaa
     9661 cctgatggat ccgcatgtga ttgtgctggg cggcggcatg tccaacagca ccgcactcta
     9721 tacccaggtg gcgccattgc tgccgcgcta tgtgttttcc gatcaggtca atacgcgctt
     9781 gttacaggcc aagcacggcg actccagcgg cgtgcgcggc gcggcctggc tgtggaacga
     9841 ataagcagcc ttagagaagc agggtccaaa cagcaaacac cgcgtaccag aaaactgcgg
     9901 cgcgcactaa cagctgccaa agcccgtcca ggctgctgac accgacctcg ccaatcaccg
     9961 gttccggcgt ctcaccagca gcacgcgcag taataatcgc caactgcgcc gcactgatat
    10021 cccagctgag cagttcgtgc aacaaggccc ggctgacccc agcaaagttg cccaccaggg
    10081 caaagctggc ggccagcaca cgcacgggca gccagtcgaa accgtggcgt acctgtacgg
    10141 cagcctcacg caaggtcgac aaggtggcat gttcactgac cagggcgacc agccgatacg
    10201 caagcgcagc caatggcccg agcaagccat accagaagat caccgcaaag aagctctggt
    10261 aggcctgcca caccaggtgg ccctgtacgc gctggagcaa ttcgccctct tcgtctgcct
    10321 caagcgctag atcacgccgc gccaccagat acgctgcctc ggcgtcctca cggcgccaac
    10381 tgtcacgaaa cggcccaagc gccgcgagca aatcaccgcg ccccaggctg taaatcacca
    10441 ccaacagatg caccggcaac gccagccaac cataagccac tggctccagc accagtagaa
    10501 tcagccccag cgcaagcaac ggcagcagca ccagcaaggc cagcgccaac cagggccgcc
    10561 cagcccactg cggcttgctc tcagtcgccg tcaacaagcg cagccaaggg ccatcgtgtt
    10621 gaatgggctt gcgccaggac gagaacttct cgacccatag caccagcagt agcaccagaa
    10681 aattcatatc aactccttgt cttgctgcaa tgccaggcgt acgcgctgcc agtcaaatgc
    10741 cgggccgggg tctgtcttgc ggcctggggc gacatcacta tgaccacaaa tacgttccag
    10801 cgtgatggct gggtaagccg cttgcagctg ctgaatcagc tcaatcagtg cagcatactg
    10861 cgccgccgtg aacggcaagt catcggtacc ttcaagctcg atacctaggg aaaaatcatt
    10921 acagtttccc cgctcagcaa accgtgacac gccagcatgc caggcgcgct cgttacagga
    10981 gacgaattgc gtaacagcgc catcacgctc gataaagaaa tgcgcggaca cctgcaggct
    11041 ggcaatgctg gcgaaatagg gatgctcatc cgcatccaga cggttctgaa aaaacgcctg
    11101 caccttgccc gtgccaaact gcccaggcgg caggctgatg ttatggatca ccaacagaga
    11161 aacttcgccc agcggtcgct gattgaagtt cggcgatggg cagcgctgga tgccatcgca
    11221 ccagccactg gaagggtcca gctgcataaa gactccttga acgagccacc actctagtgc
    11281 agcacccgtt caaggccaag ggcgaatgcc tgagccagca cgcagatgcg acaacgccgc
    11341 cagagcagaa gcccggcggc gttgttgtgg agcatcaatg cagaacgatc aagcgccttt
    11401 aagtttgcgc agattaccga ttaccgactc caaggcccga tcaaacagta gcgcatcatc
    11461 aagcaggcga atcgcactgc ggcggaactc tacggccaag cccatacggg ttttctccag
    11521 cactttcata ccggtacggt tgacgaagat gtacttgccg gttggcttga tcaccgctgc
    11581 caacttgcaa cgcagcttgt gctcttcatc ttcctgaaac tcgacccagc taccgactcg
    11641 caggttatcg acctgcagca gcgactcatc atcgtccggc agactgtttt ccggctcagc
    11701 ggcgcgcagc tcgcccggcg ccagcagaat gatttcttcc accacctcaa ccatcgccgg
    11761 ctcttcaacc gcgtcggcgg ctggcagctc tagcagtggc aaatcaatcc cggcctcgac
    11821 caacgccgct cgttgttcat catcaagcgc aggcatatca tcctgcggat tttcttccgg
    11881 caggctgcgc ttgaagcgct ggaaagcctg cacatgcaaa gcttccaact ggctgaaaaa
    11941 ctcaccagtg gagaacggat caaatgccgc gctggccatg ccttcacgca gcgctttaag
    12001 cagccccggc accatctcca gcaggcgcat gcgtgcttct ggatcttcat ggagctcaac
    12061 gctccagacc agatcatcca tggtcgccag cgccgcttgc cactcagccg actcaacgcc
    12121 gtgcttcagg caggtcagca tcagcacctt gctccaggct tcctgcagca agcgcaccac
    12181 cacttccggc agggtcttgc caagcaagcg ctcattcaac gcctgttcaa cttcacgccg
    12241 cgccaatgca gccttggcac taccctcttc agcatcgcga gtgcgctgct caagcagctc
    12301 actgcgacgg cgctcatcac cggtgaaggc cagaaactct gccagtagct cggagaagat
    12361 caccggatca tcgacaaagt cattaagcag acgctgcacc acctgctcga tcttctgata
    12421 caggctgtcg cgctgcgcat cgtcctgctc accccaaccc agggcggcag aggcaatttc
    12481 attgagcagg cggcgagccg gatggctgcc acgactgaag aaggtcttgt cgatcaccgc
    12541 cactttcagc atcgggatct gcagacggcc aatcagtgcc ttaagcgagt ccggcaaggt
    12601 gcggtcatcg agaataaatt cgaagagcat cgacaccagg ttgatcacat cttcatcaac
    12661 ttcaccgacc acccgggctt tgccggcttt ggcactggcg cgggtcagca aaccttccaa
    12721 ctgctcacgc agatcaaaat cgtcgctcgc ctgcaccggc gcacgctgct gcatatgcga
    12781 gagcaggcgc atcaggtcat tgctggagat cggcatggcg tcagccgggc gattgcgccg
    12841 cggaataaca gagccacgcg cctgagagag cagctcctgc agcgcaccga aaacctcctg
    12901 cacaccctca tcggcaaact ccgtcgagag ctccggtgat gcatcctggc gagtaccact
    12961 ggcggaagcg ctagcgcgcg agggattgcg tcgtgctggt gcggatttga gctcgggcag
    13021 tacgccgcct gcgatcaacg cctggttggc ttcggcatac agctgatcaa gaccgctgag
    13081 tacgtacttc tcgaacagtt taaggatgat cagcttgacc cggatctcaa cgcccaggct
    13141 gctgcacgca tcaaggaaga actcacacag gcaggtcggg ccgacagggt tgctcttgtc
    13201 gtccagcttc ttgctgacca tggcgttgaa gcgcgtggtc aggtggctga gcgccgtacc
    13261 gtcacgactg acaaccttgg cgaccatcga atccagcgcc accgactcct ccagcgcgtc
    13321 gttctgtacc agcgacaggg tgtcaaacga caccgcatcc aagggcgccg gcttgccgat
    13381 ttcatattga ttcagagtgg cgaaagactc gaagaccttc tgcagaaagc cgcgctcgat
    13441 atttttgcgc ttcaaacgca gatcgcgcat cgcctcgaaa aaagcattct gctcggtatt
    13501 gctagtggca cggtcggcca tctcgaacag cgtatcgtcc gcattgtcga acagcgcctg
    13561 caatgccgtc ttcagctgtt gcgccgcttt gtcacgcaca tttgtcagcg ccacaggcag
    13621 tcttcctacc ggcgaggtgg gcgattgctc aggggtcacc ttgctcaggt gaaccacatt
    13681 cgcatcagtc ttcattgcga gtctcctgca gactgcccct cttgggctaa ccgatctgca
    13741 cagcgatcaa ctgagcgtcg tccgtaacgt caaagtcctg gcgtcaacaa ttctaaaaag
    13801 taaagccatt atagggatgc gcgcgcgcat accaagctga cttttcctcc agacatgacc
    13861 cgcgtcacag atgcagcgcc acagcttccc aacggttacc tgcatacgac caacgcaaca
    13921 cgcatacgtt gcagctaacg aagaaacctc catcagcata gccccaccgc cgccagagaa
    13981 cagccgcaaa cactgcgcaa acagccatca atccctataa tcggcgcatt ctgctgactg
    14041 gagcccaaca tgcctaacct tatcctcgcc gacctcgcca atgaaatcga ggccaacgtg
    14101 cgccgcgcac tggcagagga tatcggcagc ggcgacatca ccgcccaact gatacccgcc
    14161 gagcgcctgg cacacgccaa ggttatcacc cgcgaatccg cagtaatctg cggcacagcc
    14221 tgggtagacg cggtattccg ccaactcgac ccgcgggtcg ccgtgcactg gcatatccag
    14281 gacggccaac gcgccgaagc caaccaagta ttgtttgaac tcgagggccc cgcccgcgcc
    14341 ctgctcagcg gcgagcgcag cgccctgaac tttctacaaa ccctctctgc tgtcgccacc
    14401 cgctgccagc actatgccga cctggtgcaa ggcaccgccg tcaaactgct cgacacccgc
    14461 aaaaccctac ccggcctacg cctggcgcag aaatacgccg tcacccaagg cgactgccac
    14521 aaccaccgca ttggcctcta cgacgccttc cttataaaag aaaaccacat cgcagcttgt
    14581 ggcggcatcg cccaagccgt aaccgccgcc cacaagattg ccccaggcaa gccagtggag
    14641 gttgaagtgg aaagtttgga agagctgcgc caggcgttag atgctggagc tgacatcatc
    14701 atgctcgatg agctgagcct ggatgaaatg cgtgaggctg tagccctcac cgccggccgc
    14761 gcaaaattgg aagcttcggg cggggtaaac gaaagcacct tgcgggaaat cgctgagaca
    14821 ggggtggact atgtgtcgat tggcgcgctg accaaggatg ttaaagcggt ggatttgtcg
    14881 atgcggttga gcctatagcc atccaacggc tctgtaggat gggttgagca aagcgatacc
    14941 catgcgcgcc atcaatgatg ggtattgctg cgctcaacca acgcggtccg agccatctac
    15001 gaatgattaa cgcgcggcct gctcaagaat cttgaagaac acggtttcca gcgatacagc
    15061 cggcttaaca gtcagcagat gaccacccac gttattaagc tgtgcaataa acgccggcaa
    15121 gtctgcctgc gacagctcgc actcaaactc catttcgcgc aactgcttgc agccatcaat
    15181 cattggctgc tctgcattaa agcgcaccag catctgatcg acccgctccg cggccagctc
    15241 acgcggcgag cgaacggtca gcagctcacc cttattgata aagccaaaac ggtcggcaat
    15301 gcgctctacg tcgtgcagca catgggaggt aaagaaaatc gccccgccct gctgcttgta
    15361 ctccgtaaga atacccacca catccttacg ccccaccggg tcaagccccg ataacggctc
    15421 atccagcacc agcaatttag gctgcaccac catggcatgg gccagcgcta cccgctgcac
    15481 attgcccttg gaaagctcac gaatacgccg cttggcattc tgcacaacag aaaagcgctc
    15541 cagccactgc atgcaccaag catcggcatc cttgcgcttg atgccataca tggacaagcc
    15601 catgcgcaga atttccagcg gggtgaattg ctcataaagc gcaggcgatt caggcacaaa
    15661 agccaagggt tggcgtgcct cggcacgccg tgcatccaca ccatagatgc gcacttcacc
    15721 ctggtagtca ttgatgatgt cgagcatcac tttgatggtg gtggacttgc cggcgccatt
    15781 agggccgaca aaaccgaaga cttcgccttc agttaggcta aagctgacat cgcgtaaggc
    15841 ctgaacttct ttgcttttgc tgcggaaggc atggctgacg ccattgaact caagaactgc
    15901 ggacactaat acccctccag gccggcaatc ttcatctttt tcaatatgat ttccccctcg
    15961 cgcaactcat acccaagcct gagagggtcg tccggcaaag ccggcaataa accatgctcg
    16021 accaattgat cgagactaac cagggcaccg ctttcggctt caaaacggcg ctgcgcctcg
    16081 cgcagagcga ccagaccttg caggcggacg gcccgctggc caagcatatc gcgcagcttg
    16141 gggtcgctgg cggcatcgcg ctgctgaatt aaatagtcca gagctagctt ttcgtcggcg
    16201 aaactttctg cctgtagcat caccaccatc ttgcgaaagc cggcagcatt gacaggcgag
    16261 cgctgggccg ccagctccag ggcacgactg gcctcggcga tatccttgtt gaagaacgcc
    16321 tggttgaagc catagaaaaa ggccggcaac tcgtcccaga agcgacactg catggcgcgg
    16381 cgcagcacct cggtgccctc gcggtccgca ccgccccagg taagcaggcc gttggccagg
    16441 taatagttgt cttcatggca ggggttgagt tcggccacta cctgctgggc acggactaga
    16501 tagccagtat tggcttgccc caagtccacc cctgtggagg ccaggcgcat ggtttccagg
    16561 ctggcggcaa gaaagcggtc gccgccgaac agcgccgcaa gaaccggcgc agcgatcacc
    16621 acccgcccct caatgatggg tgccacaggc actggcacct cggcgcgcca ctgcacggcg
    16681 acggagaaca caccgaagcc cagcagtgca agcagcagag accggtaacg atggcgagac
    16741 atactcatgc gaaacgcttg cgttgcaatg cccaaaccgc aagagccagc agggcaacta
    16801 gataagccag actggagagc agcagccaga accagtcggc cggcaagaac ttcatgctgc
    16861 cgtagagcgc gatcatgcgc acatccagcg caccgagatc tggcagcaga tagccgagca
    16921 gtccgacacc ggcgcggtag ctctcggcat cgccgactac ggaagcgttg cgaccgagca
    16981 gctcgacgat ggcggcgaag gaacgcgccg ccagcatgaa accgaaggtg cctactagga
    17041 caaaactggg ggtcgaggca acgactgcaa gcaaacaggc cagagcggtc agcagcagca
    17101 aatccactcc gacaaaaaca atgctgatca gatagtgccg ccccatggct accggcgtac
    17161 cttggacata accctggcta accagttcga ccagcagcgc cagcaacagc gccatcgcca
    17221 tcaacagacc cagagtcaat acggcaacgg caagaaaacg gccgagcaga agatgatgcc
    17281 gtgggcgcgg gtaagtcagg gagttaagaa aatagcgccg atcaaactcg cgagagagca
    17341 actcctggat catcaacacc aacaccatcg gcaacaacaa gcgcatcacc gaaagaccaa
    17401 catccagcgc cacagtggcg ggctgacggc cgctaaactg actggccagc atagcggcca
    17461 acgccagcat caataaagaa cagccggaca accataggta acgagcacgc caagccaaac
    17521 gaagtccgat tagaaaatca gatgaataaa gcataagcaa ccattgggaa tttattgagg
    17581 ggtataaaaa ctatgatgag ggaccacctt tcctggccag gcagccaaaa cgcagaacaa
    17641 actctaacca cactcccctt accgacccta tttcctaaca gtcacattaa aaaccttaaa
    17701 gccatccatg gccaattatt ccattttaaa aacacatatc aacagctacc gactaatgac
    17761 atttgcctga aaccctttat tgggcagcgg cagccgacac agcagtcaac acgctgttcg
    17821 cacaagctcc agcctgggcc ggatcagcat cgcaagtagt cccaacagag ccagcagtaa
    17881 cgctgccacc atcactgctg gcagtaaata aagtgttacc tgtggcctgg aaatttccag
    17941 tacaggttgc accgccagcc gatgatggca cagtagacgc acaggtagaa ttgttacaag
    18001 tagttgtatc cgcacccaca tctgaagacg taacagcaca agctattgtg cgatttttag
    18061 ttgtaccgcg ctgactcgcc gtaccaataa aaattgtatt atttgcgttg ctgttacagt
    18121 cgtaagcggc aacgacaccg gcggaaagag tagcactcac attggaggta atccaagtac
    18181 attggacatt ggtgaccgta ccactagccg gcagggtccc tgtgccagca gcatgcgcct
    18241 gggcaacaaa tactagcgac gcagctaacg atgcaaagag tatcttcttc atattatcta
    18301 tctcctgaaa aatcataaac cgaaccaata actacgctta gttagccatc gataccataa
    18361 gagtgataaa attccttgca tcggaattcg cagtgccgtc gctggctttt tggcgatact
    18421 cattaaagga aggaatagcg atggcagcaa gaataccgat aatcgcaaca acgatcatca
    18481 gctcaatcag ggtaaaacct ttttgtttct tgagagactt catggataag ctcctgttgt
    18541 ggttaaggtc aacgacctgc aaaggacaaa gcacaagccg tgccaaccgc tgaaagccaa
    18601 gctttaatcg tagagcgacc tgtcgcgatg atcaacacgt acatcgaaag ggcagcaact
    18661 gccgtttttt gtcaccccgc cttacccagc ttgggctact gctaaatttg agctataaga
    18721 caacgacccc acctagcaaa aagcacccaa ttaatcatga gcgaagcccc taatctgacc
    18781 ggactggccc ggcaactggt caatgccgag ctgctaagcg aaagcactgc ttcccaagcg
    18841 caagcgcaag cactgcgcaa taaaatcccg ctggtctcct atctcgtaca gaacaagctc
    18901 gccaccagca aagcggttgc tgaagtagct gccgagcaat ttggtgtgcc cttctgcgac
    18961 ctcaacgcta tagagaaaga aagccagcca cgcgacctga ttagtgaaaa gctggttcgc
    19021 cagcaccggg ttattccgct atttcgtcgc ggcaataagc tatttgttgg tgtctccgac
    19081 ccgaccaatc accaagcggt taccgatgta cagttttcca ccggtttaac caccgaaccc
    19141 ttgctggttg aagacgacaa gctgaccgat gccatcgaaa aattcttcga taccgccaat
    19201 agcggcatgg aagaactggg cgatgtcgac ctcgacggcg tagatgtcga agctgtaaat
    19261 gatgacaagc aggacggcaa tgacggcaac tcagcagacg acgccccggt tgtgcgtttt
    19321 gtccataaga tgctgttgga tgcgatcaaa aaaggctcgt ccgatctgca ctttgagccc
    19381 tacgagaaaa cctaccgcgt gcgcttccgc accgacggta tccttcagga aattgctcgc
    19441 ccccccatcc agttggcacc gcgcatttct gctcgcctta aggtcatggc cggcctggat
    19501 atttccgagc gacgcaagcc gcaggatgga cgcattaaaa tgcgcatctc caaaaacaaa
    19561 tccatcgact tccgcatgag cacttgcccg accttgtggg gcgagaagat cgtgatgcgg
    19621 attctcgact cggccagcgc gcagatgggc atcgacgcct tgggctatga agagtcgcaa
    19681 aaagacctgt atatgaccgc cctcaagcaa ccgcagggca tgattctggt gaccggcccg
    19741 acgggctccg gcaaaaccgt atcgctctac accggcttga atattctcaa caccgtggat
    19801 gtgaatatct ccacagccga agacccggta gagatcaacc tggaaggcat caaccaggtc
    19861 aacgttaacc ccaagcaggg catggacttt accgccgcac tgaaagcctt cctgcgccag
    19921 gacccggaca ttatcatggt gggggagatc cgcgacctgg ataccgcatc tattgcggta
    19981 aaggcagcac aaaccggcca tatggtgatg tcgaccctgc acaccaacag cgccgcggaa
    20041 accctgactc gcttgcgcaa tatgggcgtg ccgtcgttca acatcgccac ctcggtcaac
    20101 ctgatcatcg cccagcgcct ggcacgcaag ctgtgcagcc actgcaaaaa agaagtgaaa
    20161 atccctcgcg aagctctgct cgaagaaggt ttcccgaccg acaaagtcga cacgcttaaa
    20221 ctttacggcc ctgtaggctg tgaaaactgc aacggcggtt ataaaggccg tgtcggaata
    20281 tatgaagtgg ttaaaaacac gccggccctg cagcggatta tcatggagga aggtaactcc
    20341 atcgatattt ccgtacaaat gcgcaaagac ggcttcaatg acctgcgcac ctccgcttta
    20401 ctgaaagcaa tgcagggcat cactagcctt gaagaagtca accgtgtgac caaggattaa
    20461 gccatggcgg ccaaagctcc aagcatcagc acctttgcgt gggaaggtac cgataaaaaa
    20521 ggtgcaaaga tcaagggtga aatcaacggc caaaacccgg ccttggtcaa ggcgcaattg
    20581 cgcaaacaag gcatcaatcc gcttaaggtc aagaagaaac ccaagccgct cttcagtgct
    20641 ggcaagaaga tcaaacccat ggacatcgcc ctgtttactc ggcagatggc aaccatgatg
    20701 aaggccggtg ttccgttgtt gcaatccttc gatattatcg gcgagggctt cgataatccg
    20761 aaaatgcgca cactgattaa tgacgttaaa caggaggtcg cagcgggcaa tagctttgct
    20821 gcctcactgc gcacccggcc gcaatatttt gatgatctgt attgcaacct cgtggactcc
    20881 ggcgagcaat ccggtgcttt ggatacccta ctggaccgaa tcgccaccta taaggaaaag
    20941 accgaagcgc tgaaagccaa gatcaaaaag gccatgacct accctatttc ggtactggtc
    21001 gtagccgtca tcgtcacggc aatccttttg atcaaggtgg ttccgcagtt cgaaagcgtg
    21061 tttgctggtt ttggcgcaga gctcccggca tttactcaag tcgtggtagg tatgtcacgc
    21121 ggcttgcaag agtggtggta tctcttttta ttggctttct ttgtaatcgg ctttacgttt
    21181 aaagaggcgc acaagcgttc tgaaaaattc cgtgactttt tagatcgcac cctactcaaa
    21241 gcccccatta ttggcgacat cctctacaaa tccgtcgtag cacgctttgc tcgtacgcta
    21301 tctactacgt tttcggcggg tgttccgcta gtagaaggcc tgcaatcagt tgaaggggct
    21361 gccggcaacg tcgtattccg tcaagcagtg gccaaaatcc gcaacgatgt atcgtctggc
    21421 actcagctca acttctccat gcgcactacc ggcgtattcc ccagcatggc catacaaatg
    21481 accgccatcg gtgaagaatc tggctcactg gatgaaatgc tggacaagac agccgagtat
    21541 tacgaggccg aagtggataa cgctgtagac aacctgacaa ccttgatgga gccattgatt
    21601 atgtcggtac tcggggttct ggtcggcggc ttgatcattg ccatgtatct gccaattttc
    21661 cagctcggct cggtggttag ctaacttatg atccccgacc ttctggccgg caatttgccg
    21721 gcctttgttt gtgtaggcct gctgctcggt cttttaattg gtagcttcct caacgtggtg
    21781 gtttaccgct tacccatcat gatgcagcgg ggctggcgcg agcaggcccg tgagatatta
    21841 gagctgcctg ccgaacctca gaccgcaaca ttcaatttgg tattgcccaa ctcaagctgc
    21901 ccgcactgcg cccatgaaat aaagccatgg gaaaacattc cggttatcag ctacctgttt
    21961 ctgcgcggca agtgttctag ctgtaaagca cctatcagcc tgagctaccc cttggttgaa
    22021 ctaacctgcg gactgctgtc ggcctttata gcctggcact tcggttttac ctggcaggcg
    22081 ggcgcgatgc tgctgctaac ctgggggctg ctggcgatga gcctgatcga tgttgatcac
    22141 cagttgctgc cggactcgct ggtgctaccg ctgctgtggt tcggtttgat tgccaatagc
    22201 tttggcctgt ttaccagcct ggaagatgcg ctgtggggag cgattgccgg ctacttgagc
    22261 ctgtggtcgg tgtactggct gttcaagctg gtaaccggca aggaagggat gggctatggc
    22321 gacttcaagt tactggccat gctcggcgcc tggggtggtt ggcaggtatt gcccttgacg
    22381 atattgctgt cttcgctggt tggagcagtg ttgggcctta ttatgttgcg cctgcgcaac
    22441 gctgaaacca gcacgccgat ccctttcggc ccctatctgg cgattgccgg ttggattgcc
    22501 ttgctctggg gcgagcaaat cacctccagc tacctgcaga ttgccggatt ctaagctttt
    22561 gtaggagcca gcctgctgtg gttcggcact ccgacgatgc ggttatcgcc agcaacttag
    22621 tccgtatgca actcgcgcaa tccatcccgg attgcatccg ggctaaggca ctataggagg
    22681 cgctttagcg gcgagttttt aaatgaccag cgcggctaaa gctcctccct acgccggact
    22741 caacggctct gataatgcgc gcaaaacgac cgcgttcgcc agcagccaag cagctgcact
    22801 acaatcgcgc ccctgaatga ccgacacgag cgcgcacctt gacgacaaaa ccctggattc
    22861 tcggccttac cggcggcatt ggcagcggca agagcgctgt ggcccagcac tttatcgacc
    22921 tgggcgtgca cctggtcgat gccgaccacg cagcgcgttg ggtggttgag cctggtaagc
    22981 cggcgctggc caagatcacc gagcacttcg gcagcgatgt gttgcaggcc gatggccagc
    23041 ttgatcgcac agcactgcgc agccggattt ttgccgatgc ggatgagcgc cgctggctcg
    23101 aagccctgct gcatccgctg atcagccagg aaatcagcca gtatctggcg cgggcagaat
    23161 caccttacgc cattctggtc tcgccgttgc tggttgagtc aggccaacac aagttgaccc
    23221 agcgcatact ggtggtggac gcccccgagc agctgcaaat gcagcgcacc atggcccgtg
    23281 accagagttc caacgagcaa gtacaggcca ttctcaaggc ccaggccagc cgtgaggaac
    23341 gcctgcgcca cgcccatgac gtgctgctca atgatcaaga cttgccctgg ctgcgagccg
    23401 aagtggagcg actccacacc ttttacctga gcctacgcgg aggccaagca tgagtacgcc
    23461 aaccctagtc gcctgcccca cctgcagcgc acccgtcgag tggggcccgc aaagcccgag
    23521 caggccattc tgctccgaac gctgcaagct gatcgacctc ggcgcctggg cctcggaaga
    23581 acatgcgatt gccggcaacc cactggagga tgatgtgttc tctggcgaac tgccaccgcg
    23641 cgggcactaa cagactgttg agacggtcac gctgaattgg ctgttgtgac tgtggaagcg
    23701 ggcttgccca cacatcgaga ttaagcaaaa gcgtcgcggg catggcccgc tcctacagag
    23761 atcgcagaac tcgcccgcat tgcagacaat cccatgcagg ctctgtggga ggggctttag
    23821 ccgcgattgc ctagaagctg cgccccaaac gcggccaaag cgcctcccac aagaacgaca
    23881 tattatccgt gtcgcgccac tcagtagtca tggccgcata aaactgtaat cgcgctcgtc
    23941 atccaagtta tcagcgagaa actgcagttc tgcagccaga tctgccgtac tgcgtccggc
    24001 tcggctacgc tcgatcacgg cactgagcaa agcccgcagc gccatagcct gatcaaaacc
    24061 ctgcgcctcg actccccgca agctctgctc gattgcttgc cttgcccatt gatgcacgcc
    24121 catatcacgc cctctctgtt gatagatgaa agatcgcgca tggccgttcg cgcagcttga
    24181 tctggatcaa cccgcacaac ccaatcactc aactgaaggt cacgtcatca gcgcccccaa
    24241 agagaggtgc caacatggaa taccgcagga ctgtaggagc cagcttgctg gcgatcaata
    24301 aatcaaaggc atcgtcggca tgccgaacca cagcaagctg gcctctacaa aaacaaaagg
    24361 acagacccta gccatccttc cagggcgcag ccagataacg cgtgcggttg aaggtctcca
    24421 gccaatccgg atagaacacc accagcgccg ttaccagcat gccgttgata aaggcctcgg
    24481 ggaaaatcac cagccagagg tagccgataa aatcttccag ccaggatggc ataggaaaca
    24541 acccgtcaac ccagagcacc gtaaaggcgg cggcgataca cagcaatgcc gccagcgccg
    24601 cagcgaagaa gccactgcag aagatgtaga cgaacagatt gcgcggctgc tttttctcga
    24661 cccacagcgc gcaggcctcg gtcacccata cggggatcac caccagcagc acgccgttaa
    24721 cgccaagcgc ggcaaaatcc tgcttaccca gcgccagcaa gcccagctgc gcaatcaacc
    24781 ccaccagcat ggccagcggc caatccagca gcaaggtgac tgccgtcatg ccgataaagt
    24841 gataggacag gcccgagtcg aaatcccggc gtaccaacca cagtacaaac agcgccagcg
    24901 ccgtgccacc gagcagatgc tggcggcgca gatcactgca cagctcgacc cagggcgcac
    24961 gccagatcgc ccagcaaagc agcggtaaat agatcagcca gcccaggatc tgactggtgg
    25021 aagacagcaa gtgtgcggca atcatgcaaa acaactccca agccatcgcg gcctacctga
    25081 gcagtctaca accgctttag ccacccaaac tcagccaaca caaaaaagct ctgcccccat
    25141 tgcgtgtcgg aaggcgctcc gctgagaagc gcaggggcga aacccaatga tcgatgccaa
    25201 ctccagttgg gcttcgctgc gctcagcgcc aacctaccgc gtgtatgcaa tatgtaatgg
    25261 gtacatagcc tgcgaaaaag cctgcaacaa gcaccgcaga ggggtaccaa caacctgacc
    25321 gcgcaggcta agctgagccc atggatgact cagattacct gcgcctactg acgcatcagg
    25381 ccgaacaagc caacgccttt ctctccaacg cgcgcaagtg ggagcgcgag cgctgggtct
    25441 gccagcgcct gctgcaagcg ctgaatgtca gccaccgcct ggatgaattc agcgccgcca
    25501 accatgaacc gcctgacgtg ttgttccgtg aggccagctt tgaagtgttt ttcgtcctcg
    25561 acgagggccg ccgcctgaat gatgaatggc gcgccgaact ggagcggcgc cgcagtgcgt
    25621 tttccctcag ccaactggtg cgccgtgaag ccaaaccacg gcgtatcgct gctgccgagc
    25681 tgcaacagcg gctgacgcca accctacgca agaaagccca caactataaa gagcgcggcc
    25741 tcgacctggg cgagctggac ctgctcgcct acgtcaacct gaagcgcgcc gtgccggact
    25801 tcaatagcca cttcccgccg cccaccgagt ttctccgcca gggttggcgc tcgctatcac
    25861 tggtcggccc gacctttgcg cgcgtgctgt tcgcccatcc ggacgctccg gactttctgc
    25921 gcaacaacct cggccgctct gtgctgttcg atgtgggcat cagcctgtga gcccattgca
    25981 cgaactgctc gcggccgtgc cgcaacaagg ctgcgtgcgc tggattggcg tacgccctgc
    26041 ctcgcgagca gaaatgattg agttggatgc cgtcgaagca cgccgcgaag caggcctcac
    26101 cggcgaccac agccgcccag ggccacgcaa cgccaggcag gtcaccctga ttcaatggga
    26161 acacgtggcg gtgattagcg ccttgatggg gcgccctgcc gagcaaccga tcagcgcgcc
    26221 tgaactgcgg cgcaatatcg ccatcagcgg catcaacctg ttcagcctca agggccggcg
    26281 ctttcgcctt ggccaggcga tcctggaaac caccggctgg tgccaaccct gcgcaaaact
    26341 ggaagaacgc ctgggcctcg gcacctttca ggccatgcgc ggccacggcg gaattactgc
    26401 gcgcgtgcta caagggggca ttattcgcct gaatgacgcc ctgcgcgttg agcctttata
    26461 agctgcgcgg ctagaatgcc cagcacttca gcctccaatc gatccatatt aattgccaac
    26521 cggcccctta attcgaggca cgtatgtcca gtcgcctgaa tcctgatgat caaaaacgcg
    26581 tcgagcaata cctcagcgct ccgcagcatc aggtcgagcg ccagccattc aaggtttggc
    26641 ggctgctgct gattatcgtg accatcacga ttggcctggg tctgctgagc cgtctcctga
    26701 gtcgactggt gctatgagct gcttagcgct cgcccacagc gactcaccac tttttcttaa
    26761 aagccttgtg agcgtgtctg atgactcatc aaatcgtaat tgtcggcggt ggcgccggcg
    26821 gcttggagct tgctacccgc ctgggtagaa ccccgggtaa acgcggccag gccaggatca
    26881 ccctggtaga cgccaacctg acgcatatct ggaaaccgct gctgcacgaa gtggccgctg
    26941 gctcactgaa ctcctcgggt gacgaactga actacgtggc gcaagccaag tggaatcatt
    27001 tcgagttcca actggggcgg atgtgcggcc tcaatcgcgc ggaaaagcgc atcagcctgg
    27061 ccgccaccct ggatgacgat ggccaggtgc tgatgcctgc gcgcgagctg agctatgaca
    27121 gcctggtcat cgccgtgggc agcaccacca atgatttcgg cactgccggc gccgccgaac
    27181 actgcctgtt tctcgacacc cgcgaacagg ccgagcgttt ccatcgccaa ctgctcagcc
    27241 attatctgcg cgcccacgcc agccagagtg cagccaccga gctgatcaac atcgccatcg
    27301 tcggcgctgg cgccacgggc gttgaactgg cggccgagct gcaccatgcc gcgcacgagt
    27361 tggcggccta tggcctgaat cggatcagcc cggaaaacat gcgtatcacc ctgattgagg
    27421 ccggcccgcg cgtattgcca gccctgccag aacgcatcag ccgaccggtg cataagactc
    27481 tggagaagct cggggtaacc gtgctcactg gcgccgccgt cagccaggtc acccgcgagg
    27541 cgctgcatac cgagcagggt caggtcattc cggccacgct gaaagtctgg gcagcaggga
    27601 ttcgcgcccc tgacttcctt aaagagctgg atggactgga aagtaatcgc atcaaccagc
    27661 tgcatgtacg cctgacgctg cagaccaccc tggacgacaa catctttgct tttggcgact
    27721 gcgccgcctg cccacaacct ggcagcgaag gacgcaacgt accgccacgc gcacaagcgg
    27781 cgcaccagca agcctcgttg ctggccaaat cgctgaagct gcgtattgag ggcaaaacgc
    27841 taccggaata ccgctaccgc gattacggct cactgatctc actgtcgacc ttctcagcgg
    27901 ttggcaacct gatgggcaac ctgaccggca gcgtcatgct cgaatgctgg ctggccagga
    27961 tgttctacgt gtcgctctac cgcatgcacc agatcgccct gtatggcata acccgcacgc
    28021 tgctgatgat gctcggcgac cgcatcggcc gcagcaccga gccacgtctc aagctgcact
    28081 gaggtatatc tgcagggccc gtaggagcgt aatgctgttc acttaagcat cgggtggacg
    28141 cgatcatccc ctcgcccctc cggggagagg gttagggaga ggggaaggcc tccagtttgg
    28201 cggtgttttc agccctcacc cccagcccct ctcccataaa tgggaga
//
LOCUS       DASRUM010000078         3174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_700146, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000078 DASRUM010000000
VERSION     DASRUM010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700146"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..2046)
                     /locus_tag="VFF22_05570"
     CDS             complement(172..2046)
                     /locus_tag="VFF22_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX16317.1"
                     /translation="MTEQSQFALLGKKRFLPFFITQLLGAFNDNIFKQSLILAILFKI
                     SSGTDKALLINLCALLFILPFFLFSALGGQFGEKFAKDSLIRKIKFAEIVIMLLGAVG
                     VLLGNLPMMLAVLFAMGTQSALFGPVKYSILPQHLEEQELVGGNALVEMGTFLAILAG
                     TIGAGIIMASSGYAHIVAACVVLVAVFGYLASRGIPAASAAMPQLKLDWNILRQSWLI
                     MKLGLGQRPAVSRSLVGNSWFWFLGAVYLTQIPAYAKEWLYGDESVVTLILTVFSVGI
                     ALGSMLCERMSGHKVEIGLVPFGSIGLTLFGILLWWFSGGFPEAAVAHDWLGVLSIGQ
                     AWWILGSILGIGLFGGFYIVPLYALIQSRTVEHERARVIAANNILNALFMVISAIVSI
                     LFLSVAELSIPQLFLTISLMNIAVNSYIFKIVPEFSMRFMIWLLSHSMYRVEHKGLEA
                     IPDEGAAVLVCNHVSFVDALLIGGAVRRPVRFVMYYKIYNLPVLNFIFRTAGTVPIAG
                     RNEDLLIYDAAFKKIAEYLRNGEVVCIFPEGKLTTDGELNEFKNGIERIVEANPVPVI
                     PMALQGLWGSFFSRDPHKGLFKRLWSRITLVAGTPVAPELVKRELLQAQVAELRGERR
                     "
     gene            complement(2176..2505)
                     /locus_tag="VFF22_05575"
     CDS             complement(2176..2505)
                     /locus_tag="VFF22_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HZX16318.1"
                     /translation="MAIWLLILGMALITFAIRYSLFAFPDLRFPPLVRQGLHYVPTAV
                     LSAIVLPGMLIPDGQHWAFSLDNAYLLAGLATIAIAALTRHLLATIGGGLLVFFMVRW
                     LLGQLPL"
     gene            complement(2505..>3174)
                     /locus_tag="VFF22_05580"
     CDS             complement(2505..>3174)
                     /locus_tag="VFF22_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HZX16319.1"
                     /translation="PMIVGILPFGLIYGALASLAGLSLGQALGMSLLVYAGSAQFIAI
                     NLLTLGSGAVVILLTTLVVNLRHVLYSAALQPYVGRLPQRWRVPLAFGLTDETFAVVQ
                     RRYLARGMTEHSQWYHAGVALALYLSWVSSSLVGALFGQSVPNLAGWGLDFAMLATFI
                     GIVVPALRNQPQIAAALVAGAVALLCHVWPYKLGLMAAALSGITVGVRLERCKTAVTV
                     GEGA"
BASE COUNT          773 a         1010 c          885 g          506 t
ORIGIN      
        1 tctcccattt atgggagagg ggctgggggt gagggctgaa aacaccgcca aactggaggc
       61 cttcccctct ccctaaccct ctccccggag gggcgagggg atgatcgcgt ccacccgatg
      121 cttaagtgaa cagcattacg ccaatttggc ggactttttc atctctgcgg tttaacgccg
      181 ctcacctcgc aactcagcca cctgggcctg cagcaactcg cgcttgacca gttcgggcgc
      241 taccggcgta ccggccacca gagtgatgcg cgaccacagc cgcttgaaca agcccttgtg
      301 cggatcacgg ctgaaaaagc tgccccacaa gccctgtagc gccattggaa tcaccggcac
      361 cgggttggcc tcgacgatgc gctcgatgcc attcttgaac tcattcagct cgccatcggt
      421 ggtcagcttg ccctcaggga agatgcacac cacttcgccg ttacgcaggt attcggcgat
      481 tttcttgaac gcggcgtcat agatcagcag gtcttcgttg cgcccagcaa ttggcacggt
      541 gccggcggtg cggaagataa agttgagtac cggcagattg taaatcttgt agtacatgac
      601 aaaacgcacc ggccggcgca ccgcaccacc gatcagcagg gcatcgacaa acgacacgtg
      661 gttgcacacc agcaccgctg cgccttcatc agggatcgcc tccagcccct tatgctccac
      721 ccggtacatc gagtggctga gcagccagat cataaagcgc atgctgaatt cgggaacgat
      781 cttgaagatg tagctgttga ccgcgatgtt catcagcgaa atggtcagga acagctgcgg
      841 aatcgacagt tcggccacgc tgagaaacag gatcgagaca atcgccgaga tcaccataaa
      901 cagcgcatta aggatgttgt tggccgcgat cacccgcgcc cgctcatgct ccaccgtgcg
      961 cgactggatc agcgcgtaca acggcacgat ataaaagccg ccaaacaggc cgatgccgag
     1021 gatcgagccg agaatccacc aggcctggcc aatgctcagc accccgagcc agtcatgcgc
     1081 caccgccgct tcggggaagc caccggagaa ccaccagagc agaatgccga acagggtcag
     1141 accgatcgag ccgaatggca ccaggccaat ttcaaccttg tgcccgctca tacgctcgca
     1201 aagcatcgag cccagtgcga tgccgaccga gaacaccgtg agaatcagcg tcaccacgct
     1261 ttcatcgccg tacagccatt ctttggcata ggcgggaatc tgcgtcaggt acaccgcgcc
     1321 gagaaaccag aaccaggagt ttcccaccag cgaacgcgac acggctgggc gctggcccag
     1381 gccgagtttc atgatcagcc aggactgacg caggatgttc caatccagct tgagctgcgg
     1441 catcgccgcc gatgccgcag gaatgccgcg actggccagg tagccgaaca ccgccaccag
     1501 caccacacag gctgcgacga tatgcgcgta gccgctgctg gccatgatga tgcccgcgcc
     1561 aatggtgcca gcgagaatgg ccaggaacgt gcccatttcc accagcgcgt tgccgcccac
     1621 caactcctgc tcttcaaggt gctgcggcaa aatcgaatac tttaccgggc cgaacagcgc
     1681 cgactgcgtg cccatggcaa acagcacggc gagcatcatg ggcaggttgc cgagcagcac
     1741 accgacggcg ccgagcagca tgatcacgat ctcggcgaac ttgatcttgc ggatcagcga
     1801 gtccttggcg aacttttcac caaactggcc accgagggcc gagaacagga agaacggcag
     1861 gataaacagc agcgcgcaga ggttgatcag caaggccttg tcggtcccgg agctgatctt
     1921 gaacagaatg gcgagaatca gtgattgctt aaagatgttg tcgttgaaag cacccagcag
     1981 ctgagtgatg aaaaacggca aaaagcgctt tttacccagc aatgcgaatt gtgattgttc
     2041 ggtcatcgtc cgtgtacctg tctgcagtgg ccaagcgggt tgtcaggcat ttgactgcaa
     2101 gaacacctca caaagccaca gcgaaacctg ccttttgctg cattttccac aaatgcaggg
     2161 ggcaaataac cctgcttaaa ggggcaactg acccaacaac caacgcacca taaagaacac
     2221 cagcagaccg ccgccgatgg tagccagcag atgccgggtc agcgcggcga tggcaatagt
     2281 cgccagcccc gccaacaggt aggcattgtc cagactgaac gcccagtgct ggccatcggg
     2341 aatcagcatg cccggcagta cgatggcgct cagcacggcg gtcggcacgt aatgcagacc
     2401 ctggcgcacc agcggcggga aacgcagatc gggaaaggca aacaggctgt agcgaatcgc
     2461 aaaggtgatc agcgccatgc cgagaatcag cagccagata gccatcacgc gccctccccg
     2521 acagtcacgg ccgttttgca gcgctccagc cgtacgccga ccgtaatacc gctgagtgca
     2581 gccgccatca gcccaagctt gtacggccag acgtggcaca gcagcgccac cgcaccggct
     2641 accagagctg cagcaatctg tggttgattg cgcagcgccg gcaccacaat accgataaag
     2701 gtcgccagca tggcgaagtc cagcccccag cccgccaggt tcggcacact ctggccaaac
     2761 aacgcaccga ccagcgaact cgacacccag gacaggtaca gcgccaacgc cacaccggcg
     2821 tgataccact ggctatgctc agtcattccg cgagccagat aacgccgctg caccacggca
     2881 aaggtctcat cggtcaggcc aaacgccaac ggcacccgcc aacgctgtgg caaacggccg
     2941 acataaggct gcagcgccgc gctgtagagc acatgacgca gattgaccac caaggtggtg
     3001 agcagaatta ccaccgcgcc gctgcccagg gtcagcaggt tgatggcaat aaattgcgcg
     3061 gaaccggcat acaccagcag ggacataccc agcgcctgcc ccaggctcag cccagccagg
     3121 ctggccaatg cgccatagat cagaccaaac ggcagaatgc cgacaatcat cggg
//
LOCUS       DASRUM010000079         8907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2210946, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000079 DASRUM010000000
VERSION     DASRUM010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8907
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2210946"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="VFF22_05585"
     CDS             complement(<1..1345)
                     /locus_tag="VFF22_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HZX16320.1"
                     /translation="MLTLYHAPDLETLGELATTLLATPMREPFAPALVVVPSQGMGRW
                     LTLELARKQGIAMQLEVQLPAKFVWDLSRLALGQLPEQSAFSPSSLSWRLYDWLCEPA
                     NLAEAPRLAHYLDGGDERRRLSLAAKIADVFDQYLLYRDDWLAAWERNELLKLGPDEA
                     WQALLWRELTRDGHPHRARLLEELLARLHQSEPMPGLPERVLVFGISSLPPHHMRVLE
                     GLARHTQVIIFALNPCREAWGEIRDIRELARQPQSAAANQEISPDDWYLDVGHPLLAS
                     LGKQGRDFFDSLFSLASGEGSQEIGLYSEDADLHDASLLQALQNDILRLRTRTADERL
                     VLLEDDRSLELHIAHSPLREVEILHDQLLARFAAEPGLTPDQVVVLTPDIERYAPYIE
                     AVFAPREGVPRIPFSLADRSLRAEIPLLEAFLNLLALPDSRFAAEEILAWLEQPAI"
     gene            1701..3335
                     /locus_tag="VFF22_05590"
     CDS             1701..3335
                     /locus_tag="VFF22_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HZX16321.1"
                     /translation="MLTLLNLLSAVALLIWGTHIVRTGILRVYGSHLRKVLSHNVSKR
                     PLAFAAGIGVTALVQSSNATALLVTSFVAQGLMALTPALAIMLGADVGTALMARVLTL
                     DLSWLSPLLIFLGVVFFLSRKQTRAGQLGRVAIGLGLMLLALELIVAAATPITQEQGI
                     RVLFASLTGDLLLDALVGALFALISYSSLAAVLLTATLTGAGLISLPVAIGLVIGANI
                     GSGVLAFLTSSLQTPAGRRVALGSLLYKLIGLLLVMPFLDPLANWLDSLKWRPEELVI
                     AFHLLYNSLRCLLMLPTVGLMARFCNWLLPDRPDETGIARPRHLDPTALATPSLALAN
                     AVRETLRIGDLIETMLNHLLEVLSGGQTALGKELRRLDDDVDALYSAVKLYLAQVPRE
                     VLSEHDSRRWAEIIELAVNLEQAGDIIERMLGKVQDQKTAQRHSFSDSGLEELTALHA
                     QLLANLRLGLSVFLSADKESARQLLREKRRFRAQERRLAHAHVSRLHHQVVQSIETSA
                     LHMELIADMKRLNSLFCASAYVVLESSETGALHVEE"
     gene            3448..5304
                     /locus_tag="VFF22_05595"
     CDS             3448..5304
                     /locus_tag="VFF22_05595"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZX16322.1"
                     /translation="MHTPRSRPRLVGWRLLLIALLAIPAGALAQVLDVTRMPSAALSL
                     TTAVELLEDPQRRLSLTDVQSPANAARFVGDMPPAAALALGFTRSAYWLRITLGNSSD
                     VTLERMLVVDNPRISHVQIHLPNVHGQYQSISTGCDTPFETRIYPNRNFVFPLSLPAQ
                     SEQVIYLRLESTVGLLVPLQLWTPKAFHTYERGDYLAQAWYFGMASAMILFNLMLFIA
                     LRERIYLFYITFVTCTVCTVAIKNGMIPAWPGSGLNLHSNVVYYSGASLTVSTLLLFM
                     RHMLETQRLTPRVDRLLQGLVVLFLLSPLVFALALPVVARMTILLYLASAALILVVVL
                     MCAYKRQRSAYFFLGAFALLIFGSAMTLLRALGIVPTNAFTVDGVQLGSTMEMLLLAF
                     ALADRLNVMRKEKARVQVELLHAQQQLVDSLKISERELEQRVAQRTDELQVLNSKLEA
                     LSLTDSLTGIANRRHFDMLLQQEWQRAQRLGQPLALGMLDVDRFKDYNDHYGHLAGDT
                     CLHEVAQALAANLNRSSDVVARFGGEEFVFISPMSDAQDALRIAQRVVQAVAELALPH
                     AGSPIGHVTVSVGVVAMLPGPESSTEGMLRRADAALYQAKNNGRNCAELAAD"
     gene            complement(5366..7921)
                     /locus_tag="VFF22_05600"
     CDS             complement(5366..7921)
                     /locus_tag="VFF22_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HZX16323.1"
                     /translation="MHDKQLSTEELIAQDVGARTPLGAMAQVITGLALLWSLFQLWIA
                     SPLPFVFGIGVFNDTETRSIHLAFALLLAFLAYPAFRKSPRDRVPLLDVALGLVAAAS
                     AAYLFIFYQQLALRPGSLTTADMLTACIGIPLLLEATRRVLGPPLAIIALLFLVYSLA
                     GPYMPGMLAHRGVSFNAMANHQWITTEGVFGIALGVSTSFVFLFVLFGALLERAGAGH
                     YFIQLAFSMLGHFRGGPAKAAVVASGLTGLISGSSIANVVTTGTFTIPMMKRTGFSAE
                     KAGAVEVASSVNGQIMPPVMGAAAFLMVEYIGMPYVEIIKHAFLPAAISYIALLYIVH
                     LESLKLGLQPVPGHQPKPWLRRLTGFAFGAALISGLSLAVYYGLGWLKPALGEYALPG
                     VGALLALIYLGLLKIAASVPVLPAEDPDAPLDKLPQTRAVLLSGLHFLLPVVVLVWCL
                     MIERLSPGLSAFWGSVMLVIILLTQRPLLTWLRTDGTHAHGSFMDGVIDLREGLIAGA
                     RNMIGIGIATAAAGIIVGAVSQTGVGLVLADLVELLSMGNLLLMLVLTAIFSLVLGMG
                     LPTTANYIVVSSLLVPVIVTLGQQNGLIVPLIAVHLFVFYFGIMADVTPPVGLASFAA
                     AAVSKGDPIKTGIQAFYYSMRTAVLPFLFIFNTDLLLIDVDFWHGVLIFIIATVAMLI
                     FAAGSQGYFLVRSRWYENLLLLLVAFTLFRPGFWMDIVHDPYRDIPPAQLAQALGDVA
                     DGSQLRLRIKGEDAVGDAREFSLLLPVPEGATGEERLEKLGLLTYEEGGKVLVDSVTF
                     GSPAAEAGLEFDQEILNVRAPTDRWTKELMWIPGFMLFGLVIWLQRRRRKLEA"
     gene            complement(8103..>8907)
                     /locus_tag="VFF22_05605"
     CDS             complement(8103..>8907)
                     /locus_tag="VFF22_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZX16324.1"
                     /translation="AEHGIKCNAPASGGGVANVNGIRSGEFNFGIMQSDHQFKAMKGV
                     APFEKEGAMEDIRAVFSLQSEVFTILARRDANITNFDDLKGKRVNIGNPGSGQRDTLE
                     EIMQVKGWDRSAFSLAAELKPAEQASALGDNNIDAMTYFVGHPNGAIQEATTTTDAVL
                     VPVTGAEIDKLLAAKSYYTKADIPGGVYKGNDAPTPSIGGKAVLSTSAKASPEVVYQL
                     VKSVFDNIDRFKRLHPAFADLKEEDMIKVGLSAELHEGAVRYYKERGWL"
BASE COUNT         1742 a         2802 c         2731 g         1632 t
ORIGIN      
        1 cgatggccgg ctgttccagc caggcgagga tttcctcggc ggcgaagcgg ctgtccggca
       61 gcgccagcag attgaggaag gcttccagca gggggatttc cgcgcgcagg ctgcggtcgg
      121 ccaggctgaa aggaatgcgc ggcacgcctt cacggggagc gaacaccgcc tcgatatagg
      181 gcgcgtaacg ctcgatatcc ggggtcagca ccaccacctg atccggggtc aggccgggtt
      241 cggcggcaaa gcgcgcgagc agctggtcat gcaggatttc cacctcgcgc agcggcgagt
      301 gggcgatatg cagctccagc gagcggtcgt cctcgagcag cactaggcgc tcatcggcgg
      361 tgcgggtgcg caggcgcagg atgtcgttct gcagcgcctg caacaggctg gcgtcgtgca
      421 ggtcagcatc ctcggaatac aggccgattt cctggctgcc ttcgccggag gccaggctga
      481 acaggctgtc gaaaaagtcg cggccctgct tgcccagact ggcgagcagc ggatggccga
      541 catccaaata ccaatcgtcc ggacttatct cttgattagc cgctgcgctt tgcggttgtc
      601 gcgccaattc acggatatcg cgaatctcac cccaggcttc gcggcacgga ttgagggcga
      661 aaataatcac ctgggtgtgc cgcgccaggc cttccagcac gcgcatatgg tgcggtggca
      721 gtgagctgat gccgaacacc agcacgcgct cgggcaaacc cggcatgggt tcagattgat
      781 gcaaacgggc gagcagctct tccagcaaac gggcgcgatg cggatgaccg tcgcgggtca
      841 gctcgcgcca gagcagcgcc tgccatgctt cgtccgggcc gagtttaagc agctcgttgc
      901 gctcccaggc ggccagccag tcgtcgcgat acagcaggta ttggtcgaat acgtcggcga
      961 tcttggcggc aagtgacagt cgccgccgtt catcgccacc gtccaggtaa tgcgccaggc
     1021 gcggcgcctc ggccagattg gccggctcgc atagccagtc atacagccgc cagctaaggc
     1081 tgctggggct aaaggccgat tgctctggca actggcccaa ggctaggcgc gataggtccc
     1141 agacaaactt ggccggcagc tgcacctcca gttgcatggc aatgccctgc ttgcgcgcca
     1201 gctccaaggt cagccaacgt cccataccct gactcggcac caccaccagc gccggcgcaa
     1261 aaggctcgcg catcggcgtg gcgagcagcg tggtggccag ctcgccaagg gtttccaggt
     1321 caggggcgtg ataaagcgta agcatcggag ggggtcgctt ccgttcagta gcgggcaaga
     1381 acctgcttac gacctgctgc gcgtcggcct ggcggcgtta aaaacagcct cggaatgctc
     1441 atttacagct cgtaaactcc gcttcctcgg ctgtttttgc cttgcccggc tctagctcgc
     1501 tagatcgtaa acaggttcta aggttatcag ctcacgcagc ctggcgggat tgcacgacag
     1561 tctgtgtcgc gcgctggcgc agagtgccaa ccccagctaa agctaaatac agggtctgcg
     1621 cttctacaag atgggccttt ataacaagta aaaaccacgc gtcacgctgc aacagggctg
     1681 acgcgccagg gagcagcacc atgctcaccc tgcttaatct tctgtctgcc gtcgccttgc
     1741 tgatctgggg cacccatatc gtccgtaccg gcatcctgcg ggtgtatggc tcgcacctgc
     1801 gcaaggtgct tagccataac gtcagcaagc ggccgctggc gtttgccgcc ggcatcggcg
     1861 ttacggcgct ggtgcagagc agcaacgcca ccgcgctgct ggtcacctcg ttcgttgccc
     1921 agggcctgat ggcgttgact ccagcgctgg cgattatgct cggtgcggat gtcggtacgg
     1981 cgctaatggc gcgagtgctg accctcgacc tgagctggct gagcccgttg ctgatctttc
     2041 tcggtgtggt gttctttctc tcgcgcaagc agacccgcgc cggccagctc ggccgcgtgg
     2101 ccatcggcct ggggctgatg ctgctggccc tggagctgat cgtcgccgcc gccacgccga
     2161 ttacccagga gcaaggcatc cgcgtgctgt tcgcctcgct gaccggcgac ctgctgctgg
     2221 atgctctggt cggcgcgctg tttgcgctga tttcctactc cagcctggcc gccgtgctgc
     2281 ttaccgcgac gctgaccggt gctggcttga tcagcctgcc ggtggccatc ggcctggtga
     2341 ttggcgccaa catcggcagc ggcgtgctgg cctttctcac cagcagcctg caaaccccgg
     2401 cgggccgccg tgtggccttg ggtagcctgc tgtacaagct gattggcctg ctgctggtga
     2461 tgccgttcct cgatccactg gccaactggc tggacagcct gaagtggcgg cccgaggagc
     2521 tggtgatcgc cttccacctg ctctacaaca gcctgcgctg cctgctgatg ctgcccacag
     2581 tcgggctgat ggcacgcttc tgcaactggc tgctacccga tcgcccggat gaaaccggca
     2641 tcgcccgccc gcgccacctc gaccctaccg cgctggccac accgagcctg gcgctagcca
     2701 atgcggtgcg cgaaaccctg cgcatcggcg acctgatcga gaccatgctc aaccatttgc
     2761 tggaagtcct gagtggcggg caaaccgccc tgggcaagga gctgcgccgc ctggatgacg
     2821 atgtcgatgc gctctacagc gcggtcaaac tctacctggc gcaggtgcca cgcgaggtgt
     2881 tgagtgagca cgacagccgg cgctgggcgg agatcatcga gctggcagta aacctggagc
     2941 aggccggcga catcatcgaa cgcatgctcg gcaaagtgca ggatcagaaa actgcgcagc
     3001 gccattcgtt ttcagacagc ggcctggagg aactcaccgc cctgcatgcc cagctgctgg
     3061 ccaacctgcg cctgggcctc tcggtgtttc tctccgccga caaggaaagc gcgcgccagc
     3121 tgctgcgcga gaaacgccgc ttccgcgccc aggaacgccg cttggcccac gcccacgtca
     3181 gccggctgca tcatcaggtg gtgcaaagca tcgaaaccag cgccctgcac atggagctaa
     3241 tcgccgatat gaagcgcctc aactcactgt tctgcgccag cgcctatgtg gttttggaaa
     3301 gcagcgagac gggggcgctg catgtggagg aataggccag gcctcgcggg ttatggaagg
     3361 tgtgcgcaat cggcccctct tgtagactgc gcgaacgttg cagccttcgg cacctcagtg
     3421 ctgattgacc attgacgggt agctcgcatg catacgcctc gttctcggcc acgcttagtc
     3481 ggctggcgcc tactgcttat cgcgctgctg gctatcccgg ccggggctct ggcacaggtg
     3541 ctggatgtga cccgcatgcc gtccgcagcg ctgtcgctga ccacggccgt cgagttgctg
     3601 gaagacccgc agcgccgcct aagcctgaca gacgtacagt cgccggcgaa cgccgcgcgc
     3661 ttcgtcggcg atatgccgcc cgcagcagca ctggcgctcg gctttacccg ctcggcctac
     3721 tggttacgca tcaccctggg caacagtagc gacgtgacgc tggagcgcat gctggtggtc
     3781 gacaatccgc gcatttccca cgtccagata catctgccca acgtacatgg ccaataccag
     3841 tcaatcagca ccggctgtga cacacccttt gaaacgcgga tctaccccaa tcgcaacttc
     3901 gtctttcccc tgagcctacc ggcccagagt gagcaggtga tctacctgcg cctggaatcc
     3961 accgtcggcc tgctggtgcc cctgcagctc tggacgccaa aagccttcca cacttacgag
     4021 cgtggtgact acctggcaca agcgtggtac ttcggcatgg ccagcgccat gatccttttc
     4081 aacctgatgc tgtttattgc cctgcgcgaa cgcatctacc tgttctacat cacttttgtg
     4141 acctgcacgg tatgcaccgt ggcgatcaag aacggcatga tcccagcatg gcccgggagt
     4201 gggcttaacc tgcactccaa tgtggtctac tactcgggcg cctcgctgac cgtcagtaca
     4261 ctgctgctgt ttatgcggca catgctggaa acgcagcgcc tcacgccacg agtggatcgg
     4321 ctgctgcagg gcctggttgt gttgtttctg ctgtcgccgc tggtgtttgc gctggcgttg
     4381 ccggtggtcg cacgcatgac catcctgctt tacctggcca gcgcggcttt gatcttggtg
     4441 gtggtgttga tgtgcgccta caagcgccag cgcagtgcct atttctttct tggcgccttt
     4501 gccctgctca tcttcggcag cgccatgacc ttgctcagag cgctagggat agtgccaacc
     4561 aatgctttta ccgtggacgg tgtccagctg ggctcgacca tggagatgct gttgctggcc
     4621 tttgctctgg ctgatcgcct caacgtgatg cgtaaagaga aggccagggt gcaggtagaa
     4681 ctgttgcacg cccagcaaca gctggtggac agcctgaaaa tctccgagcg cgaattggag
     4741 cagcgagtgg cgcaacgcac cgatgaactg caggttctca acagcaagct tgaggcgtta
     4801 agcctgaccg acagcctgac cgggatcgcc aaccgtagac acttcgatat gctcctgcag
     4861 caggaatggc agcgcgccca acgcctgggc cagccactgg ctctgggcat gctggatgtg
     4921 gatcgtttca aagactacaa cgaccactat ggccatctgg ccggcgatac ctgcctgcac
     4981 gaggtggccc aggcactcgc ggccaaccta aatcgcagca gcgacgtggt ggctcgcttt
     5041 ggcggtgaag agttcgtctt tatcagcccc atgagcgatg cccaggatgc tctgcgcatc
     5101 gcgcaacggg tcgttcaggc agtcgccgaa ctcgccttgc cccacgcggg ctcacccatt
     5161 ggccatgtga cggtgagtgt cggggtggtg gccatgttgc caggccccga gagctccacc
     5221 gaaggcatgc tcaggcgtgc cgatgccgcg ctctatcagg ccaagaacaa tggccgcaat
     5281 tgtgcggaac tggcggcaga ctaaccccac aaacaacaaa ggcagcccaa gctgcctttg
     5341 ttgttaacca cgaccgaacc gcagctcagg cttctaactt acgccggcga cgctgcaacc
     5401 agatcaccag accgaacagc ataaagcccg gaatccacat cagctccttg gtccagcgat
     5461 ccgtcggggc gcggacgttg aggatttcct gatcgaactc caggccggct tcggctgccg
     5521 ggctgccgaa ggtgacgctg tcgaccagca ccttgccacc ctcttcatag gtcagcaggc
     5581 caagtttctc caggcgctct tcaccagtgg caccttccgg cacgggcagc agcaggctga
     5641 actcgcgggc atcgcccact gcatcctcac ctttgatgcg caagcgcagt tggctgccat
     5701 cggctacatc acccagggcc tgggccagtt gtgccggcgg gatatcgcgg taagggtcgt
     5761 gcacgatgtc catccagaaa cccggacgga acagggtgaa cgccaccagc aacaacagca
     5821 ggttttcgta ccagcgactg cgcaccagga agtaaccctg gctgccggcg gcgaagatca
     5881 gcatggcgac ggtggcgatg ataaagatca gcacgccgtg ccagaaatcc acgtcgatca
     5941 gcagcagatc ggtgttgaag ataaacagga acggcaacac cgccgtacgc atgctgtagt
     6001 agaacgcctg aatcccggtc ttgatcggat cgcccttgga caccgcagca gcggcgaacg
     6061 aggccaggcc caccggcggc gtcacgtcgg ccatgatgcc gaagtagaag acgaacagat
     6121 gcaccgcgat cagcggcacg atcaggccgt tctgctgacc caacgtgaca atcaccggca
     6181 ccagcaggct ggacaccaca atgtagttgg cagtggtcgg cagacccatt cccagcacca
     6241 ggctgaagat cgccgtcagc accagcatca gcagcagatt gcccatcgac agcagttcga
     6301 ccaggtcggc cagcaccaga ccaaccccgg tttgcgacac cgcgccgacg atgatgcccg
     6361 ccgccgcagt ggcaatgcca atgccaatca tattgcgcgc gccagcaatc aggccttcac
     6421 gcaggtcgat cacaccgtcc ataaacgagc cgtgggcatg ggtgccatcg gtgcgcagcc
     6481 aggtcagcag cggacgctgg gtcagcagga taatcaccag catcacgctg ccccagaagg
     6541 ccgacaggcc gggggacaag cgctcgatca tcaggcacca gaccagcacc actaccggca
     6601 acagaaagtg caggccggaa agcagtacgg cgcgggtttg cggcagcttg tcgagcggcg
     6661 cgtccggatc ttcggcgggc aacaccggca cgctggcggc aattttcaac aggcccaggt
     6721 agatcagcgc cagcagcgcg ccgacacctg gtagggcgta ttcacccaac gccggtttga
     6781 gccagccgag gccgtaatac accgccagcg acagaccgct gatcagcgct gcgccgaagg
     6841 cgaaaccggt caggcggcgt agccagggct tgggttgatg tccaggcacg ggctgcaggc
     6901 cgagcttcag cgactccaga tggacgatat acagcagggc gatataggaa atcgccgccg
     6961 gcaggaaggc gtgcttgatg atctcgacat agggcatgcc gatgtactcg accatcagaa
     7021 aggccgctgc acccatcacc ggtggcatga tctggccatt taccgacgac gccacttcca
     7081 ccgcaccggc cttctcggcc gagaaaccgg tgcgcttcat catcggaatg gtgaaggtgc
     7141 cggtggtcac cacgttggca atcgacgagc cggaaatcag cccggtcagc cccgatgcca
     7201 ccacggccgc cttggccggg ccaccacgga agtggccgag catgctgaag gccaactgaa
     7261 taaagtaatg ccccgcgcca gcgcgttcga gcaaggcgcc aaacagcacg aagagaaaca
     7321 cgaagctggt ggatacgccg agggcgatgc cgaacacccc ttcggtggtg atccactggt
     7381 ggttggccat ggcattaaag ctgaccccac ggtgcgccag cattcccggc atatacggcc
     7441 cggccaggct gtaaacgagg aacagcaggg cgatgatcgc cagtggcggg ccgagcactc
     7501 ggcgggtggc ttccagcagc aacgggatgc cgatgcacgc ggtcagcatg tccgcggtgg
     7561 tcaggctacc ggggcgcaag gccagctgct ggtagaagat aaataggtag gcggcgctgg
     7621 cggcagcgac caggcctaga gcgacatcca gcagcggcac gcgatcacgc ggcgacttgc
     7681 gaaaggccgg gtaggcgaga aacgccaata acagggcaaa cgccaggtgg atagagcggg
     7741 tttcggtgtc gttgaacaca ccaataccga acacgaacgg cagcggcgag gcgatccata
     7801 gttgaaacag cgaccagagc agggccaggc cggtgataac ctgggccatg gcccccaatg
     7861 gggtgcgcgc accgacatcc tgggcaatca gttcttcggt ggacagttgc ttgtcatgca
     7921 tgggttgaac ctgcattcga gagtacggaa aacggcaaaa cccgcagcct tgcagctgcg
     7981 ggtttgctta tctgaaaaca ctagcccgtt tgcacgggta gtcacgtagc ccgaataagc
     8041 ctaggcgcaa tccgggaaac gcggtcccgg attgcgctgg cgcttatccg ggctacacag
     8101 gcctacagcc agccgcgctc tttgtagtaa cgcactgcgc cttcatgcag ctctgcgcta
     8161 aggccgactt tgatcatgtc ttcttctttc aggtcggcaa atgcagggtg cagacgcttg
     8221 aagcgatcaa tattgtcgaa taccgattta accagctggt agaccacttc cgggctggcc
     8281 ttggccgagg tggaaagcac cgctttgccg ccaatcgacg gggttggcgc gtcgttgccc
     8341 ttgtacacgc cgcctgggat atcagctttg gtgtagtagc tcttcgcagc cagcagcttg
     8401 tcgatttccg caccggtcac cggcaccagt accgcatcgg tagttgtggt ggcttcctgg
     8461 atggcaccgt tcgggtggcc aacgaagtag gtcatcgcat cgatgttgtt atcacccagg
     8521 gcagaagcct gctcggctgg cttcagctcg gccgccaggg agaacgcaga acgatcccag
     8581 cctttgacct gcatgatctc ttccagggtg tcgcgctgac cggaacctgg gttgccgatg
     8641 tttacgcgct tgcctttgag gtcatcgaag ttagtgatgt tggcatcacg gcgggcgagg
     8701 atggtgaaca cctcactctg cagcgagaac accgcacgga tgtcctccat ggcgccttct
     8761 ttctcaaacg gcgcaacgcc cttcatggcc ttgaactggt ggtccgactg catgatgccg
     8821 aaattgaatt cgccactgcg gatgccgttg acgttggcca cgccaccgcc actggctggg
     8881 gcgttgcact tgatgccgtg ttcagca
//
LOCUS       DASRUM010000080        10140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2046128, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000080 DASRUM010000000
VERSION     DASRUM010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 10140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10140
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2046128"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFF22_05610"
     CDS             complement(<1..237)
                     /locus_tag="VFF22_05610"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZX16325.1"
                     /translation="MARATARHILVASEDKCNELKAAIEGGADFAQVAKDNSSCPSSR
                     SGGDLGSFGPGQMVKEFDTVVFSAPINVVQGPVKT"
     gene            complement(364..1581)
                     /gene="sstT"
                     /locus_tag="VFF22_05615"
     CDS             complement(364..1581)
                     /gene="sstT"
                     /locus_tag="VFF22_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HZX16326.1"
                     /translation="MTTAQHPLLQLLNRTSLVLQIAIGLVAGIALALFLPEAAASVSF
                     LGTVFVSALKAVAPVLVFVLVAASIANHQQGQQTHIKPILLLYLIGTFSAALIAVAAS
                     FAFPSSLTLVSNSAELSPPGGISEVLKTLLLNVVDNPINALLKGNFIGILAWAIALGF
                     AFRHASPSSKQMLADLSDAVTSIVKLVIRLAPLGVFGLVAATLAESGMSALLDYLHLL
                     LVLVGCMLFVALVSNPAIVYFKTRRNPYPLVLTCLRESGLTAFFTRSSAANIPVNLQL
                     CQRLGLHEETYSVSIPLGATINMAGAAITITVLTMAAVHTLGIAVDLPTALLLSLLAS
                     ISACGASGVAGGSLLLIPLACSLFGIPNDVAMQVVAVGFIIGIVQDSAETALNSSTDV
                     LFTAAACQAEQRD"
     gene            1857..2519
                     /locus_tag="VFF22_05620"
     CDS             1857..2519
                     /locus_tag="VFF22_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003369295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HZX16327.1"
                     /translation="MSNEDANAMAVETLTIVEARVLGCLIEKQLTTPEAYPLTLNALV
                     LACNQKTSREPVLNLTAGQVGQALRSLEGREMARLVMGSRADRWEHRADKALELVAAQ
                     VVLIGLLLLRGPQTINELLTRSSRMHDFDDAEQVQHHLERLISRELACLLPRQSGQRE
                     DRYMHVLGDPADLQAVLAARASEPQRATAGGQQDERLEALEARIAALEERLAQLESPL
                     NA"
     gene            complement(2580..3230)
                     /locus_tag="VFF22_05625"
     CDS             complement(2580..3230)
                     /locus_tag="VFF22_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16328.1"
                     /translation="MINKELTQLSIPSMITMPSLHFFIKFSSVLLAPYSLITALTRSL
                     NMKKQILTAVILSSLSISAFALPQNNPQPLLGEMQDSSVKTLMGFGSNLQTEGGAERL
                     QERLRVAESSADRTYKPQAEHMQVVESSADRTFTPQMEHLRVAESSADRTYKPQAERM
                     QVAESSADRTFTPQMEHLRVAESSADRTYKPQIEHMQVAESSADRTYKPQTPGQTA"
     gene            3542..4783
                     /locus_tag="VFF22_05630"
     CDS             3542..4783
                     /locus_tag="VFF22_05630"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HZX16329.1"
                     /translation="MTAEGFLEGRRLQLARGVLLQCIMGGYPAPCYYGSDDGDLALNA
                     EPTQIHRLPWSETPRALAICDADELDGRSSGLSTRGLLKQVIARYASHGWQPVVATEL
                     EFFVFAHNADPLQPFQPPLGLDGRREDGCSAFSVSSNNGLRPFFREVYQCMEALGLPR
                     DTFMHEMGVSQFEINLLHGDPLLLADQTFLFKHLLKEVALKHGLTVVCMAKPLAHTPG
                     SSMHIHQSVVEQGSGQNIFSAADGQATPAFYQFIAGQQAAMADFTLLFAPHVNSYQRL
                     CHPYASPNNACWSEDNRAAGLRIPASAPVARRVENRLPGADANPYLALAASLAAGLYG
                     IEQQLQPSAPIQGEFEVPDELALPCTMHAAIERLKRSTLALELFGKEFIEGYIASKTL
                     ELTSFFDEITPWERRVLAAHA"
     gene            4838..6166
                     /locus_tag="VFF22_05635"
     CDS             4838..6166
                     /locus_tag="VFF22_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX16330.1"
                     /translation="MSVILKSFSSLYFATLLMLLGSGLLSTYLALRLAETADGLWVGA
                     LMAANYLGLVLGGKIGHRLIARVGHIRAYVACAGVVTAAVLGHGLSDWLPSWLLLRML
                     VGLGMMCQYMVIESWLNEQAETSQRGQVFAGYMGASYLGLILGQLVLVVHPTLGLELL
                     MLVALCFALCLVPVALTRKLHPAPLHPAPLEMRFFIDRVPLSLTTIVVAGLLIGSFYG
                     LAPLYGTRMGLSTEQIGLFMGFCIIAGLVVQWPLGWLSDRHDRVQLIRGCAALLALAA
                     LPLAILPQASLPLLFAGGFAVCALQFSLYPLAVALANDHVEAERRVSLTAMLLVTFGV
                     GASIGPLLAGVLMRFLGANMLYAFVCGCALILVLRIRPERVTHLHQVDDAPLHHIPMP
                     DSTTSSPLTAALDPRVDEQVVHEQMHDSGSVQEPSAEPAAESPAQSELRG"
     gene            6163..6855
                     /locus_tag="VFF22_05640"
     CDS             6163..6855
                     /locus_tag="VFF22_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HZX16331.1"
                     /translation="MTPLISVVIPVRNEAQALPGLLDDLADFRATGAELILVDGGSGD
                     GTCELALGRVDQLLRTAPGRALQMNAGAAVARGEYLWFVHGDTRISAESLSSLQKALS
                     ERPLWGRFDVRLSGPGLALRVIGAMISLRSRLSGIASGDQGIFVAHQHFEALGGYAQI
                     PLMEDLQLCRRLKTLARPRCLRPPLSTSSRRWEQNGIWRTVLLMWCLRLAYYCGASPE
                     KLVRQYRRGSLL"
     gene            6852..7463
                     /locus_tag="VFF22_05645"
     CDS             6852..7463
                     /locus_tag="VFF22_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HZX16332.1"
                     /translation="MNLSISLHMLARAPVAGRVKTRLIPALGAQGACDLQQLLLERAL
                     QLPAHGFSERFLWLDDFPGADLQQLAQCLGWTLVEQPAGDLGERMRRIATLGLAESDA
                     VVLIGNDCPALDGDYLQAACGALQEHSVVIGPAEDGGYVLLGLRSMAASLFSDMPWGT
                     ERVFDLTLQRLQQLDWRPALLPALWDVDRPEDLSRLAALNILI"
     gene            complement(7460..8209)
                     /locus_tag="VFF22_05650"
     CDS             complement(7460..8209)
                     /locus_tag="VFF22_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar regulator YcgR PilZN domain-containing
                     protein"
                     /protein_id="HZX16333.1"
                     /translation="MSSPFSQEDGPQPPKVLKAPVEIVANLRQLQQNHDPLIITFHER
                     NQRFQSYVIEVDRDKKLLILDELMPSDGERYLANGEAFNVEAFHEGVRVAWKCEQNVR
                     ITEYEGARCYFSPMPLEVTYHQRRSAFRAPLKQADQIKVELAGDKLRTALPGLLLDIS
                     ATGCKLRFPGNISSSLQAGQVYERFTARFPFGAMTTAIEMRHVQYEDKVDTTFVGARF
                     HRINGQEQRLVERFVYQLQREARRVESDGLF"
     gene            complement(8291..8758)
                     /locus_tag="VFF22_05655"
     CDS             complement(8291..8758)
                     /locus_tag="VFF22_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HZX16334.1"
                     /translation="MHDTSLLELFSNDIGTAEQLLELIDTEFQALSDRDLPRLQNILT
                     EKLPLLTLLDQHGKERSQLLASLNLSPDRTGLQALAAQSNQGKLLLERGDQLNALLER
                     CQAANLRNGRLIRSSQASTNSMLGILRGNETPSLYDSRGSAARIGQQRPLSQA"
     gene            complement(8834..9178)
                     /gene="flgM"
                     /locus_tag="VFF22_05660"
     CDS             complement(8834..9178)
                     /gene="flgM"
                     /locus_tag="VFF22_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014719583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HZX16335.1"
                     /translation="MVIDINRLNNAATPANSGRTGSAQSGGQNEAIGSKQPSNVPSPV
                     EQAQQAGKSGESVQLSREAQQLQKVSEKLRDQPTVNKERVAELKQAIEDGSYQVDSQR
                     VASKLLNFESQR"
     gene            complement(9336..10094)
                     /gene="flgA"
                     /locus_tag="VFF22_05665"
     CDS             complement(9336..10094)
                     /gene="flgA"
                     /locus_tag="VFF22_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003175636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HZX16336.1"
                     /translation="MNTETTTNRHSLAKCRYWLAVVPALLALGYSAVATANATRPEQL
                     IGAGEAFLEQAVSDYLQRSNIAGRHEIQINRLDPRLRLPLCPQPLTTTLESPAEPIGR
                     VTLRVRCDGAAPWTVFVPGQVRLYREVVIASRPLKRDNLLTDMDVTLAERDVGLLNQG
                     YLTALKQAIGKKLTRPLLPDQVLAPIHVQLAEAIRKGDQVVISARSGGISVRMPGEAL
                     SDGTIGRQISVRNQRSNRVIRARVVGPGQVEVAM"
BASE COUNT         1798 a         2737 c         3296 g         2309 t
ORIGIN      
        1 ggtcttcacc gggccttgca ccacgttgat cggtgcgctg aatactacgg tgtcgaattc
       61 cttgaccatc tggcctgggc cgaacgagcc aaggtcaccg ccgctgcggc tggacgggca
      121 gctggagttg tctttggcga cctgggcgaa gtcggcaccg ccttcgattg cggctttcag
      181 ttcgttgcac ttgtcttcgc tggcaaccag gatgtgacgg gcagtggctc gggccatggg
      241 agttactcct tataaagaag cggcaaagcc taccggaatc aaccggtcag gcaaacgccg
      301 cagggatgac tggtgagaaa aaggcttacc catgggtaag ccttttagtg tttcggcaga
      361 tgcttagtcg cgttgttcgg cctggcaggc ggctgcggtg aacagcacgt cggtggagga
      421 gttcagcgcg gtttccgcag agtcctggac gatgccgatg ataaagccga cagcgaccac
      481 ctgcatggcc acatcattgg ggatgccgaa caggctgcaa gccagcggaa tcagcagcag
      541 cgagccgccg gccacgcccg aggcgccgca ggcgctgatc gaggcgagca ggctgagcag
      601 caaggctgtc ggcagatcga cggcgatgcc cagggtgtgc accgcggcca tggtcagtac
      661 ggtgatggtg atcgctgcgc cggccatatt gatggtcgcg cccagtggga tcgacaccga
      721 gtaggtttcc tcatgcaggc ccaggcgttg gcagagctgc aggttgaccg ggatattggc
      781 cgccgagcta cgggtaaaga acgctgtcag gccgctttcg cgcaggcagg tcagcaccag
      841 cgggtagggg ttgcgacggg tcttgaagta gacaatcgcc gggttgctta ccagggcgac
      901 aaacagcatg caaccgacca gtaccagcag caggtgcagg taatcgagca gggcggacat
      961 gcccgactcg gccagcgtgg ccgcaaccag gccgaaaacc cccagtggtg ccaggcgaat
     1021 caccagtttg acgatgctgg tgactgcgtc ggagaggtcg gcgagcattt gcttgctgct
     1081 cgggctggcg tggcgaaagg caaagccgag ggcaatggcc caggccagga tgccgataaa
     1141 gttgcctttg agcagggcat tgatcgggtt atccaccaca ttcagcagca gggttttcag
     1201 cacttcgctg atgccgccgg gtggcgacag ctcggcgctg ttgctcacca gggtcaggct
     1261 ggacgggaag gcaaagctgg cggccaccgc gatcagcgct gcgctgaagg tgccgatcag
     1321 gtacagcagc agaatcggtt tgatatgggt ctgctggcct tgctgatggt tggcaatcga
     1381 cgccgctacc agtacgaata ccagcaccgg agccacggcc ttcagcgccg agacaaacac
     1441 cgtgccgagg aagctgacgc tcgcggcagc ttcaggcaga aacagcgcca gggcgatacc
     1501 ggccaccagg ccgatagcaa tttgcagcac caggctggtg cggttgagca gttgtaaaag
     1561 cgggtgttgg gcagtggtca tgacatgcat ctctacttgg cgggcaggcc tgcgatctct
     1621 tcatattgcc tgccgggctt ggggcgccgg gaggctggat acatggctgc ggtgcgcgtt
     1681 atgaggcttt tcgcggggga tgaaacaagg ccacgctgaa aaatagcgcg cgactctacc
     1741 acagtgaccc gctgttacgc gctggcgcag tgccagcccc gcccagcggc ggttatgcct
     1801 atcaaccggc cgtcaggcgg gctaggctag ggcctttgcg aggccctgag gttgttatgt
     1861 ccaacgaaga tgcgaacgct atggcggttg aaaccctgac gatcgtcgag gcacgggtgc
     1921 tgggttgcct gatcgaaaag cagctgacca cccccgaagc ctatcccctg acgctgaacg
     1981 ccctggtgct ggcctgcaat cagaaaacca gccgtgagcc ggtgctcaac cttaccgccg
     2041 gccaggtcgg ccaggctctg cgtagcctgg aagggcgaga aatggcgcgc ctggttatgg
     2101 gcagccgggc ggatcgctgg gaacaccgcg ccgacaaagc gctggaactg gtggcggcgc
     2161 aggtggtgct gatcggtctg ttgctgctgc gtggcccgca gaccatcaac gagctgctga
     2221 cccgcagcag ccgcatgcat gattttgatg atgccgagca ggtgcagcat cacctcgaac
     2281 gtttgatcag tcgcgaactg gcttgcctgt tgccacgcca gtcggggcag cgtgaagacc
     2341 gttatatgca cgtgctgggt gacccagcgg atttgcaggc ggtattggca gccagagcca
     2401 gtgagccgca gcgcgcgacg gcgggcggtc agcaggatga gcgcctggaa gctctggagg
     2461 cgcggattgc ggcgctggaa gagcgcttgg cgcagttgga atcgccccta aacgcataaa
     2521 gcacccatcg ggtgctttgt gcgagaagtg tcggagggtt agctgacacg gctgcttgct
     2581 taggcagttt gtcctggggt ttgcggtttg taagtgcggt cggcactgct ttccgctacc
     2641 tgcatgtgct ctatctgggg cttgtaggta cgatcagcac tgctttcggc aacacgcagg
     2701 tgttccatct gcggtgtaaa agtgcgatcg gcactgcttt ccgctacctg catacgctcc
     2761 gcctggggct tataggtacg atcagcactg ctttcggcga cacgcaggtg ttccatctgc
     2821 ggtgtaaaag tgcgatcggc actgctttcc actacctgca tatgctccgc ctggggctta
     2881 taagtgcgat cagcactgct ttccgcgact cgtagccgtt cttgcaggcg ctcagcgccg
     2941 ccttcagttt gcaggttgct accaaaaccc atcaaggtct ttacgctgct gtcctgcatt
     3001 tcacccagca gtggctgcgg gttgttctgt ggcaaggcaa aggcgctgat ggacaggctg
     3061 ctcaggatca cggcggtgag aatttgcttt ttcatgttca ggctcctcgt aagggcggta
     3121 atgagtgagt acggagccaa taatacggat gaaaatttga tgaagaagtg caggctgggc
     3181 atggtaatca tcgacggtat cgatagttga gttaattctt tattaatcat ttatttagaa
     3241 ttaattcatg acgcatgaaa gatgattttt ggagtgagtg tgcatgatca ttaatatcta
     3301 attaatcgat gtatttggtt gcgtagcccg cgctacaggt atcgggtgtg tctttgcttg
     3361 aaatatcgaa cggctggtgc gtattttgta cgcctcgccg tgtgactacg gcctcttgca
     3421 ttaataagga caacagcaaa tgagtaccct cgactgcacc gccctggctg agttgctgcg
     3481 tggtgttgat gaagttgaat gtgtaacgcc cgatctaaac ggcgtgccgc gcggcaaggt
     3541 gatgacggct gagggttttc tggaggggcg acgcttgcag ttggcgcgcg gggttttact
     3601 gcaatgcatc atgggtggct atccggcgcc gtgttattac ggcagcgatg acggtgacct
     3661 agccttgaat gccgagccta cgcagattca tcgcctgccc tggagtgaaa cgccgcgcgc
     3721 gctggccatc tgtgatgccg atgagctgga tggacgcagc tccgggttgt cgacccgcgg
     3781 gctgctcaag caggtaattg cgcgttacgc cagccatggc tggcagccgg tggtggcgac
     3841 cgagctggag tttttcgtgt ttgcccataa tgccgatccg ctgcagccgt ttcagccgcc
     3901 gctggggctt gatggtcggc gtgaagacgg ctgttcggct ttcagcgtga gctccaacaa
     3961 cggcctgcgt ccgttcttcc gtgaggtcta tcagtgcatg gaggcgttgg ggttgccgcg
     4021 cgataccttt atgcacgaga tgggcgtgag ccagttcgag atcaacctgc tgcatggcga
     4081 tccgctgctg ctggcggatc agacctttct gttcaagcat ctgctcaagg aggtcgcgct
     4141 caagcacggg cttactgtgg tgtgcatggc caagccattg gcgcacacgc cgggtagctc
     4201 tatgcatatt catcagagcg tggttgagca gggcagtggg cagaatatct tcagcgcggc
     4261 cgatggccag gcaacgccgg cgttctatca atttatcgcc ggtcagcagg cggccatggc
     4321 cgacttcacc ctgctatttg ctccgcatgt aaattcctat cagcgtcttt gccatcctta
     4381 tgcgtcgccg aacaacgcct gctggtccga ggataatcgc gccgctggcc tgcgcattcc
     4441 agccagtgcc cctgtggcgc gacgggtaga gaaccgtttg ccgggcgccg atgccaaccc
     4501 ttatctggcg ttggcggcga gccttgcggc aggtctgtat gggattgagc aacagctgca
     4561 gcccagcgcg ccgatccagg gtgagttcga agtaccggat gaattggcgc taccctgtac
     4621 catgcatgcc gcgattgagc gtctcaagcg cagtacgttg gcgctggaac tgttcggtaa
     4681 agagttcatc gaaggctaca ttgccagcaa gacgttggag ctaaccagct tctttgacga
     4741 aattaccccg tgggagcgcc gcgtgctggc ggcccacgct tagagtctta ccgccgaaca
     4801 tgctttcgtc ggcctttctg gagccacgtg ctgccccatg tctgtgatcc tgaagtcatt
     4861 tagttcactg tatttcgcca ccctgttgat gctgttgggc tcgggtttgt tgagtactta
     4921 cctggcgttg cggctggcgg aaacggccga tgggctgtgg gtcggtgcgc tgatggcggc
     4981 caattacctg ggactggtgc tgggcggcaa gattggccac cgcttgattg cccgggtcgg
     5041 tcatatccgc gcctatgtcg cctgtgctgg agtagtgacc gctgcagtgc ttggccatgg
     5101 cttgagcgac tggttaccca gctggttgct gctgcgtatg ctggttggct tgggcatgat
     5161 gtgccagtac atggtgatcg agagctggct caatgaacag gcggaaacca gccagcgtgg
     5221 ccaggtgttt gccggttata tgggggcttc ttatctgggc ttgatcctcg gacagctggt
     5281 gctggtggtg cacccgacgc tgggcctgga gctgctgatg ctggtggcgc tgtgttttgc
     5341 cctgtgcctg gtgccggtag ccttgacccg caagttgcac cccgcgcctt tgcatcccgc
     5401 accgctggaa atgcgcttct ttatcgaccg tgtgccgttg tcgctgacca ccatcgtggt
     5461 ggcgggcctg ctgatcggtt cgttctacgg cctggcgccg ctgtatggca cgcgcatggg
     5521 cttatccacc gagcagatcg gtctgtttat gggtttctgc atcatcgccg gcctggtggt
     5581 gcagtggccg ttgggctggc tgtccgatcg gcatgaccgc gtacagctga ttcgtggctg
     5641 cgccgcgttg ctggcgttgg cagcgctgcc cttggcgatt ctgccgcaag ccagcttgcc
     5701 gctgctgttt gccggcggct ttgccgtgtg cgccctgcaa tttagccttt acccgctggc
     5761 ggtggcgctg gccaatgacc atgtcgaagc cgagcggcgg gtatcgctga cggccatgct
     5821 gctggtgacc tttggtgttg gcgccagtat tgggccgttg ctggcgggcg tgctgatgcg
     5881 ctttctcggc gccaacatgc tgtatgcctt tgtctgtggt tgcgccttga ttctggtgct
     5941 gcgtatccgg ccagaacgcg tcactcatct gcatcaggtc gacgacgcgc cgttgcatca
     6001 catccccatg ccggacagca ccaccagctc gccgctgacc gcggcgctcg atccacgcgt
     6061 cgacgaacag gtggtacatg agcagatgca tgacagcggc tcggtgcaag aacctagtgc
     6121 agaacctgct gcggagtcgc ccgctcagtc agagctgcgc ggatgacgcc gctgattagc
     6181 gtggtgatcc ctgtgcgtaa tgaggcccag gcgttaccgg gcttgttgga tgatttggcg
     6241 gattttcgcg ctaccggcgc cgagttgatt ctggtcgacg gcggcagcgg tgatggcacc
     6301 tgcgaactgg cgctggggcg ggttgatcag ctgttgagga cggcccctgg ccgcgcgctg
     6361 cagatgaacg ccggtgcggc ggtggcccgt ggtgaatacc tgtggtttgt gcatggcgat
     6421 acgcggatta gtgctgagtc tcttagtagt ctgcaaaagg cgttaagcga gcggccgctg
     6481 tgggggcgtt ttgatgtgcg tctatccggc cctggtctgg cattacgagt gattggcgcg
     6541 atgatcagcc tgcgttcgcg cctgagcggt atcgcatcgg gtgatcaggg gatttttgtt
     6601 gctcaccagc attttgaggc cttgggtggc tatgcgcaga ttccgttaat ggaggatttg
     6661 cagctgtgcc ggcggcttaa gacactggct cgcccgcgtt gtctacgtcc accgttgtcg
     6721 acttccagtc ggcgctggga gcagaacggg atctggcgta ccgtgttgct catgtggtgt
     6781 ctgcggctgg cctattactg tggcgccagc ccggaaaagc tggttcgtca gtatcgtcga
     6841 gggtcgttgc tatgaacctg tcaatctcat tgcatatgct cgcgcgggcg cctgttgccg
     6901 ggcgggtaaa aacgcgtctg atcccagcct tgggcgctca gggcgcctgc gatttgcagc
     6961 agttgctgct ggagcgtgct ttgcagttac cggcccatgg ctttagtgag cgctttttgt
     7021 ggctggacga ttttcccggt gctgatttgc agcagttggc gcaatgcctt ggctggacgc
     7081 tggtcgagca accggctggc gatctgggtg agcgcatgcg ccgtattgct accttgggac
     7141 tggcggaaag cgatgcagtg gtccttattg gcaatgactg cccggcgctc gatggcgact
     7201 atctacaagc ggcttgcggc gccttgcagg agcattccgt ggtgatcggg ccggcagagg
     7261 atggtggtta cgtcttgctt gggctgcgta gcatggctgc cagcttgttc agcgatatgc
     7321 cgtggggcac ggaacgggtt tttgatctga cgctgcaacg cctgcagcag ctggactggc
     7381 gtccggcgct gctgcccgca ttgtgggatg tagatcggcc tgaggattta tcgcgcctcg
     7441 cggcgttgaa tatcctgatc tagaacagtc cgtcgctttc gacgcggcgc gcttcacgtt
     7501 gcaactggta gacgaagcgt tcgaccaggc gctgctcctg gccattgatg cggtggaagc
     7561 gggcgccgac aaaggtggta tcgaccttat cttcgtactg cacgtggcgc atctcaatgg
     7621 cggtggtcat ggcgccgaaa ggaaagcggg cggtaaagcg ctcgtatacc tggcctgctt
     7681 gcaggctact gctgatattg ccgggaaatc gcaatttgca gccggttgct gagatatcca
     7741 gcaatagccc gggcagggcg gtgcgcaact tatcgccggc cagctcgact ttgatctggt
     7801 cagcctgttt aagtggggcg cggaaggcgc tgcgccgttg gtggtaggtc acctcaagtg
     7861 gcatcgggct gaagtaacag cgcgcgccct catactcggt gatgcgcacg ttctgttcac
     7921 atttccaggc tacgcggaca ccttcatgga aggcctcgac attgaacgcc tcgccattgg
     7981 ccaggtagcg ctcgccgtcg ctcggcatca gttcatcgag aatcagcagc ttcttgtcgc
     8041 gatcaacctc gatcacgtag ctttggaagc gttggttgcg ttcgtggaag gtaatgatca
     8101 gtgggtcatg gttttgttgc agttggcgca ggttggcaac tatttccacc ggtgccttga
     8161 gcaccttggg tggctgcggg ccatcttcct ggctaaaagg actggacacg gtccataact
     8221 ctccgagcaa atgctatggc actagcatac tggcaatgtg acagtatgtc ccctgtgcct
     8281 tttataggtt tcaggcctgg ctgagcgggc gctgctggcc gattctagcg gcactgccgc
     8341 ggctgtcata gaggctgggt gtctcgttgc cacgcagaat gccgagcatg ctgttggtcg
     8401 aagcctggct ggagcgaatt aggcggccat tgcgcaggtt ggccgcctgg cagcgttcaa
     8461 gtagggcgtt gagttggtcg ccgcgctcaa gcaggagttt gccttggttg gactgtgcgg
     8521 caagtgcctg caaaccagtg cggtcggggc tgaggttgag gctggcgagc aattggctgc
     8581 gttctttgcc gtgctggtcg agcagggtta gcagcggcag tttttccgtc aggatgttct
     8641 gcaggcgggg caggtcgcgg tcactgaggg cttggaattc ggtgtcgatc aattcaagca
     8701 actgctcggc ggtgccgata tcgttgctga acagttcaag caggctggtg tcgtgcattg
     8761 cgggctcttc ggggtaacag acattgcatg cagtgtctat tgggcggcca attaccccag
     8821 cgtagcccgg tgactagcgc tgggattcga aattaagcag tttgctggcg acgcgctggc
     8881 tatcgacttg gtagctgccg tcttcgattg cttgtttcag ctcggcgacg cgctctttgt
     8941 tgacggtagg ctgatcgcgc agcttctcgc tgactttctg caactgctgg gcctcacggc
     9001 tcagctgtac cgactcgccg ctcttgccgg cttgttgcgc ctgctcgacg gggctgggta
     9061 cgttgctagg ttgtttgctg ccgatggctt cgttctggcc acctgactgc gcactgccgg
     9121 tccgccccga attggctggc gttgcggcat tgttgagccg gttgatgtca atgaccatga
     9181 tgcaaaacct cgagcagtgt ttggacgctt gcctagtttg tcggcattgc cccaaaaaac
     9241 tttagggcaa aaaagtgtgc gtcaggtaac agtctaaaca cagtgtggat aatcgtcagc
     9301 tcaccgagtg cacaaagtta agacaagaat catgcctaca ttgccacttc aacctgccca
     9361 gggccgacta cccgagctct gatcacccgg ttggagcgtt ggttacgtac gctgatctgc
     9421 ctgccaatag tgccgtctga cagggcttca ccgggcatac gtacacttat tccaccgcta
     9481 cgggcgctga tcaccacctg atcacccttg cgtatagctt cggctaattg cacatgaatc
     9541 ggcgcaagca cctgatcggg caacagtggt cgcgtcaatt ttttgccgat tgcttgcttg
     9601 agggcggtga gatagccctg gttgagcagg ccgacatccc gctcggccaa cgtcacatcc
     9661 atatccgtca gcaggttgtc gcgtttcagt gggcgactgg cgatcaccac ttcgcgatac
     9721 aggcgcactt gcccaggtac aaacactgtc cagggggccg cgccgtcaca gcgtacgcgc
     9781 agggttacgc ggccaatagg ctcagcaggg ctttccaggg tcgtggtcaa tggttgtggg
     9841 cacagcggca ggcgcaggcg tgggtcgagc cgattgattt gaatctcatg gcggccagcg
     9901 atgttgctac gctgtaagta atcactcacc gcttgctcaa gaaaagcctc gccagcgccg
     9961 ataagttgtt caggacgcgt ggcgttagct gttgccacgg cgctatagcc gagtgccagc
    10021 aaagcgggta caactgccag ccagtagcgg cattttgcca gtgagtgtcg gttagttgtc
    10081 gtttcagtgt tcatatgggt tggatagcaa ggcgcgtgcc gcctgctacg ctgacagcaa
//
LOCUS       DASRUM010000081         3793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_701631, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000081 DASRUM010000000
VERSION     DASRUM010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701631"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFF22_05670"
     CDS             <1..223
                     /locus_tag="VFF22_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZX16337.1"
                     /translation="VVLFIMGGIFVVETLSVIIQVASFKLTGKRVFRMAPIHHHFELK
                     GWPEPRVIVRFWIITVILVLIGLATLKLR"
     gene            245..1591
                     /gene="murD"
                     /locus_tag="VFF22_05675"
     CDS             245..1591
                     /gene="murD"
                     /locus_tag="VFF22_05675"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZX16338.1"
                     /translation="MTLIASDHFRIVVGLGKSGMSLVRFLARQGLPFAVVDTRANPPE
                     LATLREQFPQVEVRCGELDVEFLCRASELLVSPGLAIATPALQAAAARGVKLSGDIDL
                     FARYAKAPIVAITGSNAKSTVTTLVGEMAAAAGKKVAVGGNLGTPALDLLNDEIELYV
                     MELSSFQLETTDQLNAEVATCLNISEDHMDRYADLPAYHLAKHRIFRGARQVVVNRDD
                     ALSRPLVADQLPCWYFGLGKPDFKRFGLLEENGEKSLAYQFAALLPVRELKIRGAHNQ
                     SNALAALALGHAVGLPFEPMLATLKQFAGLPHRCQWVGERNAVSYYDDSKATNVGAAL
                     AAIEGLGADIAGKLVLIAGGDGKGADFSALKAPVGKFCRAVVLLGRDAELLAEALGDA
                     APLIRVKTLEEAVQRAAELAEAGDAVLLSPACASLDMFKNFEERGRLFAQAVEGLQ"
     gene            1591..2817
                     /gene="ftsW"
                     /locus_tag="VFF22_05680"
     CDS             1591..2817
                     /gene="ftsW"
                     /locus_tag="VFF22_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HZX16339.1"
                     /translation="MLARLLALPSPLHTRRGIDLDFPLLAGCLALLGLGLVMIASASS
                     EVAAVQSGNPLYHMIRHLIYLTIGLGAALATLLVPMALWQRYSGMLLLAAVVLLILVL
                     LPGIGREVNGAKRWIGLGIFNLQPSELAKLFSVMFIAAYLVRRQEEVREKLTGFIKPM
                     LVLGPIAALLLAEPDFGATVVLVGAAIAMLFLGGVNLLRFIPLAACVLGLGVLVMTSQ
                     AYRLERLTNFVDPWADQYGAGYQLSQALIAFGRGEWFGVGLGNSVQKQFYLPEAHTDF
                     VFAVLAEELGMVGALITVALFVFVSVRALYIGLWAEKAKQFFSAYVAYGLAFLWIGQV
                     LINIGVNVGLLPTKGLTLPFLSYGGSSLVICCVSLALLLRIEWERRTQLGSEDVDFKE
                     SDFFEGQSAEEVQHAR"
     gene            2807..>3793
                     /gene="murG"
                     /locus_tag="VFF22_05685"
     CDS             2807..>3793
                     /gene="murG"
                     /locus_tag="VFF22_05685"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HZX16340.1"
                     /translation="MRGNVLIMAGGTGGHVFPALACAREFKARGYSVHWLGTPRGIEN
                     ELVPAAGLPLHLIDVTGLRGKGKLSLLKAPFQLIKALFQARKVMRELQPVCVLGMGGY
                     LTGPGGLAARMSGAPLIIHEQNAVAGTANRSLVPFASRVCEAFPNTFGNSTKRRTTGN
                     PVREELFLETPRDSLRNRQPRLLVLGGSLGAEPLNKLLPEALALVTAELRPEVFHQAG
                     KQHDAITAERYRAVGVEAQVAPFIKDMARAYAWADLVVCRAGALTVSELAAAGLPAFL
                     LPLPHAIDDHQTRNADYLASAGAAVLLPQRSTDAAKLAAQLTEVLMHPEQLTQ"
BASE COUNT          595 a         1169 c         1239 g          790 t
ORIGIN      
        1 agtggtgctg ttcatcatgg gcggcatctt cgtggtggaa accctgtcgg tgatcatcca
       61 ggtcgccagc ttcaagctga ccggcaagcg cgtgttccgc atggcgccga tccatcacca
      121 ctttgaactt aaaggctggc ctgagccgcg cgtgattgtg cgcttctgga tcatcaccgt
      181 gattctggtg ctgatcggcc ttgccaccct gaaactgcgt tgaggactga ggatttataa
      241 cagcatgacc ctgatcgctt ctgaccactt ccgcatcgtt gtcggcctcg gcaagagcgg
      301 catgtccctg gtgcgcttcc tcgcgcgcca gggcctgccg tttgccgtgg tcgacacccg
      361 tgccaacccg ccggagctcg ccaccttgcg tgagcagttc ccgcaggtgg aagtgcgttg
      421 tggcgaactg gatgtggaat tcctctgccg cgccagcgag ttgctggtca gcccgggcct
      481 ggccatcgcc accccggcgc tgcaggcggc cgctgcgcgt ggcgtcaagc tgtcgggtga
      541 tatcgacctg ttcgcccgtt acgccaaggc gccgatcgtg gcgattactg gttccaacgc
      601 caaaagcacc gtgaccacct tggtcggcga gatggctgca gcggccggca agaaggtcgc
      661 cgtcggcggc aacctcggta ccccggcgct ggacctgctg aatgatgaga tcgagctgta
      721 cgtcatggaa ctgtcgagct tccagctgga aaccaccgac cagctcaacg ctgaagtggc
      781 gacctgcctg aatatcagcg aagaccatat ggatcgctac gccgatctgc cggcttatca
      841 cctggccaag caccgcatct tccgcggcgc acggcaggtg gtggtgaacc gcgatgacgc
      901 gctgtcgcgt ccgctggtgg ccgatcaact gccgtgctgg tacttcggcc tgggcaagcc
      961 agacttcaag cgctttggcc tgctcgaaga aaatggcgag aaatccctgg cgtaccagtt
     1021 cgcggcgctg ctgccggtgc gtgaactgaa aattcgcggc gcgcacaacc agtccaatgc
     1081 tctggcggcc ttggccctgg gccatgccgt gggcctgccg ttcgagccga tgttggcaac
     1141 cctcaagcag ttcgctggcc tgccgcatcg ctgccagtgg gttggcgagc gtaatgccgt
     1201 gagctactac gacgactcca aggccaccaa cgtcggtgcc gcgctggccg ccatcgaagg
     1261 cctgggggcg gatatcgccg gcaagctggt gcttatcgcc ggtggcgacg gcaagggtgc
     1321 ggatttctcg gcgctgaaag cgccggttgg caagttctgc cgcgccgtag tgctgcttgg
     1381 ccgcgatgct gaactgctgg ctgaggcgct gggcgacgcc gcaccgctga ttcgggtcaa
     1441 gactctggaa gaggctgtgc agcgcgctgc tgaactggcc gaggcgggcg atgccgtgct
     1501 gctgtcgccg gcctgcgcca gcctggacat gttcaagaat tttgaagagc gcggacgcct
     1561 gttcgcccag gccgtggagg ggttgcaatg atgcttgccc gcctgctcgc cttgccttcg
     1621 ccgctgcata cccgtcgcgg tattgatctg gacttcccgc tgttggccgg ttgcctggcg
     1681 ctgctcggcc tgggcttggt gatgattgct tcggcttcct cggaggtggc cgcggtgcag
     1741 tcgggcaacc cgctgtatca catgattcgt cacctgattt acctgaccat cggcctgggc
     1801 gctgcactgg cgaccctgct ggtgcctatg gcgctgtggc agcgctacag cggcatgctg
     1861 ctgctggcgg cggtggtgct gctgattctg gtactgctgc cgggcattgg tcgtgaggtc
     1921 aacggggcca agcgctggat cggcctgggc atcttcaatc tgcagccgtc cgagctggcc
     1981 aagctgttta gtgtgatgtt tatcgccgct tacctggtgc gtcgtcagga agaagtacgc
     2041 gagaagctca ccggctttat caaaccgatg ctggtgctgg gccctattgc cgcgctgctg
     2101 ctggccgagc cggacttcgg cgccaccgtg gtactggttg gcgctgccat cgccatgctg
     2161 ttcctcggcg gggtcaacct gctgcgcttt attccgctgg cggcctgcgt actcggcctt
     2221 ggcgtgctgg tgatgaccag ccaggcctat cgtctggagc gtctgaccaa cttcgtcgat
     2281 ccctgggctg accagtacgg cgccggttac cagctgagcc aggcgctgat tgccttcggc
     2341 cgtggcgaat ggttcggcgt cggcctgggc aacagcgtgc agaagcagtt ctacctgccg
     2401 gaagcacata ccgatttcgt atttgccgtg ctggccgaag agctgggcat ggttggcgca
     2461 ctgattaccg tcgccttgtt cgttttcgtc agtgtgcgcg ccctgtatat cggtctgtgg
     2521 gcggagaagg ccaagcagtt cttctcggcc tatgtggctt acggcttggc gttcctgtgg
     2581 atcggtcagg tgctgatcaa tattggcgtg aacgtcggcc tgctgccgac caagggcctg
     2641 actctgccat tcctcagcta cggcggcagc tcgctggtga tctgctgcgt cagcctggcg
     2701 ctgttgctgc gtatcgagtg ggagcgtcgc acccagctgg gcagcgagga tgtcgacttc
     2761 aaggaatcgg atttcttcga aggccagtct gccgaggagg ttcagcatgc gcggtaatgt
     2821 gctgatcatg gccggcggca ccggtgggca cgtattcccg gcgctggcct gcgcccgcga
     2881 attcaaggcg cgtggttaca gcgtgcattg gctgggtacg ccgcgtggta tcgagaatga
     2941 actggtgccg gctgccggtc tgccgctgca cctgatcgac gttactggcc tgcgtggcaa
     3001 gggcaagctg tcgctgctga aagcgccgtt ccagctgatc aaggcgctgt tccaggcgcg
     3061 caaagtcatg cgcgaactgc aaccagtgtg cgtgctgggc atgggtggct atctcaccgg
     3121 tccgggcggc ctggctgcgc gcatgagtgg cgcgccgctg atcatccatg agcagaatgc
     3181 cgtggccggt accgccaacc gcagcctggt gccattcgcc agccgcgtct gtgaggcctt
     3241 cccgaatacc ttcggcaaca gcaccaagcg ccgcaccacc ggtaacccgg tgcgtgaaga
     3301 gctgttcctg gaaaccccac gtgattcgct gcgcaatcgc cagccgcgtt tgctggtgct
     3361 gggcggcagc ctgggcgccg aaccgctgaa taaattgctg cctgaagcct tggcgctggt
     3421 gactgccgag ctgcgccctg aagtgttcca tcaggccggc aagcaacatg atgcaattac
     3481 cgctgaacgc tatcgcgcag ttggcgtcga agcgcaggtc gcgccgttta tcaaggacat
     3541 ggcgcgggct tacgcctggg ccgatctggt ggtgtgccgc gcaggcgcgc tgaccgtcag
     3601 cgagctggcc gccgccggcc tgccagcctt cctgctgccg ctgccgcatg cgatcgatga
     3661 tcaccagacc cgtaatgccg attatctggc cagtgccggc gctgccgtac tgctgccaca
     3721 acgttccact gacgccgcca agctggccgc gcagctgacc gaggttttga tgcaccccga
     3781 acaactcact caa
//
LOCUS       DASRUM010000082         4343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1382268, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000082 DASRUM010000000
VERSION     DASRUM010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382268"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..691
                     /locus_tag="VFF22_05690"
     CDS             47..691
                     /locus_tag="VFF22_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZX16341.1"
                     /translation="MTSIRERNRELILRAASEEFADKGFAASKTSDIAAKAGVPKPNV
                     YYYFKSKENLYREVLESIIEPLLEASAPFNQPGKPADVLRAYIRSKIRISRELPFASK
                     VFASEIMHGAPHLSADQTAQLNAQAKHNISCIQGWIDQGLMAKVDPHHLLFSIWAATQ
                     TYADFDWQISTVTGKASLDEADYEAAAQTIIRLVIKGCEIDETQPSPSEQVTNA"
     gene            complement(806..1246)
                     /locus_tag="VFF22_05695"
     CDS             complement(806..1246)
                     /locus_tag="VFF22_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020302584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZX16342.1"
                     /translation="MSTLNLQIATTISNRALAVGREISAAPLTVAVLDAGGHLISLQR
                     EDGASLLRPQIAIGKAWGALALGKGSRLIAADAQQRPAFIGAVNNLADGQLVPAPGGV
                     LIRDERGEVIGAVGITGDTSDIDEQCAVSAVASVGLKPDAGIAA"
     gene            1585..3360
                     /gene="gcl"
                     /locus_tag="VFF22_05700"
     CDS             1585..3360
                     /gene="gcl"
                     /locus_tag="VFF22_05700"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HZX16343.1"
                     /translation="MAKMRAIEAAVLVMRREGVDTAFGIPGAAINPLYAALKKVGGID
                     HVLARHVEGASHMAEGYTRTNAGNIGVCIGTSGPAGTDMVTGLYSASADSIPILCITG
                     QAPRARMHKEDFQAVDITSIVKPVTKWATTVMEPGQVPRAFQKAFYEMRSGRPGPVLI
                     DLPFDVQMAEIEFDIDAYEPLPLAKPAANRVQAEKALAMLNDAERPLLVAGGGIFNAD
                     AADKLVEFAELTGVPVVPTLMGWGAIPDDHKLMVGMVGLQTSHRYGNATLLESDLVFG
                     IGNRWANRHTGSIDVYTEGRKFIHVDIEPTQIGRVFNPDLGIVSDAGAALDAFIEVAR
                     EWKSAGKLKDRSAWVESCRERKRTLQRKTHFDNVPVKPQRVYEEMNEFFGKDTCYVST
                     IGLSQIAGAQFLHVYKPRHWINCGQAGPLGWTIPAALGVVKADPTRNVVALSGDYDFQ
                     FMIEELAVGAQFNLPYIHVVVNNSYLGLIRQSQRGFEMDYCVQLAFENINAPEINGYG
                     VDHVAVVEGLGCKAVRVFEPNDIQAALAKAKALMEEFRVPVMVEIILERVTNISMGVE
                     INAVNEFEELAERGADAPTAISLLD"
     gene            3456..4238
                     /gene="hyi"
                     /locus_tag="VFF22_05705"
     CDS             3456..4238
                     /gene="hyi"
                     /locus_tag="VFF22_05705"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HZX16344.1"
                     /translation="MPRFAANLSMLFTEVDFLDRFDAAATAGFSGVEYLFPYDFAAEV
                     IKARLDANRLEQVLFNLPAGDWAKGERGIACHPDRVEEFRAGVDQAIAYAKVLGNKQI
                     NCLAGIRPQGLDCATVEATFVDNLKFAAGKLEAAGIKLVMEMINTRDIPGFYLNTTKQ
                     ALAIRDKVASSNLYLQYDIYHMQIMEGDLARTVETNLSVINHVQLADNPGRNEPGTGE
                     INYRFLFEHLDRIGYQGWIGCEYKPATTTAAGLGWMKAHNAI"
     gene            4256..>4343
                     /locus_tag="VFF22_05710"
     CDS             4256..>4343
                     /locus_tag="VFF22_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZX16345.1"
                     /translation="MAKIGFIGTGIMGKPMAQNLQKAGHTLFF"
BASE COUNT          972 a         1363 c         1204 g          804 t
ORIGIN      
        1 cccggcgctg cccagcgacg ggcgttcgac agaagcaaag aggcttatga ccagtattcg
       61 cgagcgtaac agagagctga ttttacgcgc agccagtgag gagttcgccg acaaaggctt
      121 tgctgccagc aaaaccagcg acatcgccgc caaggccggg gtgcccaagc ccaacgtcta
      181 ctactacttc aagtcgaaag aaaacctcta ccgcgaagtg ctggaaagca ttatcgaacc
      241 gctgctggaa gcttccgcgc cgttcaatca gccaggcaaa cccgcagacg ttctgcgcgc
      301 ttatatccgc tcgaagatcc gcatctcccg cgaactgccg tttgcctcta aggtattcgc
      361 cagcgaaatc atgcacggcg cgccgcatct atccgccgac cagaccgcgc aactgaatgc
      421 ccaggccaag cacaatattt cctgcattca gggctggatc gaccagggcc tgatggccaa
      481 ggttgacccg catcacctgc tgttcagcat ctgggccgcg actcaaacct acgccgactt
      541 cgactggcag atttccaccg taaccggcaa agccagcctg gacgaagccg actacgaagc
      601 cgccgcacaa accatcatcc gcttggtgat caagggctgc gagatcgacg aaacccagcc
      661 cagtccgtcc gagcaggtca ccaacgccta agcccggtta tccgccctac ccttggctta
      721 aggcagcaat aaataaaaac ggtgaatgcc gcaaagcatt caccgttttt tttgcagctc
      781 tagttgcggc gaaagccgca catcatcagg cggcgatacc cgcatccggc ttcagcccga
      841 ccgatgccac cgcgctcacg gcgcactgct catcgatatc cgaggtatcg ccagtgatgc
      901 ccactgcgcc aattacctcg ccgcgctcat cacgaatcag cacaccaccc ggtgcaggca
      961 ccagctgacc gtccgccaga ttgtttaccg caccgataaa cgccgggcgc tgttgtgcgt
     1021 cggcggcgat caggcgcgat cccttgccca gtgccagcgc accccaagcc ttaccgatgg
     1081 cgatttgcgg gcgtagcaag ctggcgccgt cttcacgctg caggctgatc agatgcccgc
     1141 cagcatccag cacggctacg gtcagcggtg cggcggagat ttcccggcca actgccaggg
     1201 cgcggttact gatggtggtg gcgatttgca gattcaacgt actcatattg ggtcttcctc
     1261 gatgctgata aatcctacga aacacctggt gctgcgccgc ctgcagggcg attgagagcg
     1321 gccgggcaat ccaaagctca acaccactat cccactagaa acaaaaaact caatagacgc
     1381 atacaataca aacactattt gtatacagtt atccgggcta ttttccgcag cagatcaagt
     1441 accccagcta aaacggttgt tttaaaagat tttttgccag acagcgcctc gacaacaaaa
     1501 cgtgttgacc ggaaggtcag gccatgaata gaatccaagc cagctgtttt gtatacaatc
     1561 tgataacaaa cgaggcacaa gacaatggcc aaaatgagag caatcgaggc cgccgtactg
     1621 gtgatgcgcc gcgaaggcgt tgacactgcg ttcggcatcc ccggcgccgc catcaacccc
     1681 ctgtacgctg cactgaagaa agtcggcggt atcgaccacg tcctggctcg ccacgtcgaa
     1741 ggggcctcgc acatggctga gggttacacc cgcaccaatg ccggcaatat cggcgtgtgc
     1801 atcggcacct ccggcccagc tggcaccgac atggttaccg gcctgtactc ggcatcggcc
     1861 gactccatcc cgattctgtg catcaccggc caagcgccgc gtgcccgcat gcacaaggaa
     1921 gacttccagg ccgtcgacat caccagcatc gtcaagccgg tgaccaagtg ggcaaccaca
     1981 gtaatggagc cgggccaagt gccacgtgcc ttccagaaag ccttctatga aatgcgcagc
     2041 ggccgtccag gccccgtgct gatcgacctg ccgttcgacg tgcagatggc cgagatcgag
     2101 ttcgacatcg acgcctacga accactgcca ctggccaagc cggcggcaaa ccgggtgcag
     2161 gctgaaaaag ccctggccat gttgaatgac gccgagcgtc cgttgctggt tgccggtggc
     2221 ggtatcttca acgccgacgc ggccgacaag ctggttgagt tcgctgaact gaccggcgta
     2281 ccagttgtgc caaccctgat gggctggggc gcgattccgg atgaccacaa gctgatggtt
     2341 ggcatggttg gtctgcagac gtcgcaccgt tacggcaacg ccaccctgct ggaatccgat
     2401 ctggtattcg gtatcggtaa ccgttgggcc aaccgccaca ccggcagcat cgatgtgtac
     2461 accgaaggcc gcaagttcat tcacgtcgac atcgaaccga cgcagattgg ccgcgtgttc
     2521 aacccggacc tgggcatcgt ttccgatgct ggcgccgctc tggacgcctt tatcgaagtg
     2581 gcccgcgaat ggaaaagcgc tggcaagctg aaagaccgca gcgcctgggt tgaatcctgc
     2641 cgtgagcgca agcgcaccct gcagcgcaag acccacttcg ataacgtgcc ggtcaaaccg
     2701 cagcgcgtgt acgaagagat gaacgagttc ttcggcaagg acacctgcta cgtcagcacc
     2761 atcggtctgt cgcagattgc cggcgcgcag ttcctccacg tctacaaacc gcgtcactgg
     2821 atcaactgtg gccaggccgg cccgctgggc tggaccattc cggcggcact gggtgtggtc
     2881 aaggctgatc cgacccgcaa cgttgtggcg ctgtcgggcg actatgactt ccagttcatg
     2941 attgaagagc tggctgtggg cgcgcagttc aacctgccgt acatccacgt ggtggtgaac
     3001 aactcctacc tgggtctgat ccgtcagtcc cagcgcggtt tcgagatgga ctactgcgtg
     3061 cagttggcct tcgagaacat caacgcaccg gagatcaacg gttacggcgt cgaccacgtt
     3121 gccgtagtgg aaggcctggg ctgtaaagcc gtgcgtgtat tcgagccgaa cgacatccag
     3181 gcggcattgg ccaaggccaa ggcgctgatg gaagagttcc gcgtaccggt catggtcgaa
     3241 atcatcctgg agcgtgtcac caacatctcc atgggtgtgg aaatcaacgc ggtcaacgag
     3301 ttcgaagaac tagccgaacg cggtgccgac gccccgactg ccatctcact gctggattga
     3361 tgcaaccgta gggtggaaaa ccgcgaagcg ttttacaccg cctttggtgg atgtgctgcg
     3421 cgacatccac cctacgaatt ttaaaggagc acaccatgcc ccgttttgcc gccaacctgt
     3481 ccatgctgtt caccgaagtg gacttcctgg accgtttcga cgctgccgcc accgcaggtt
     3541 tcagcggtgt cgaatacctg tttccctatg acttcgctgc cgaagtgatc aaggcgcgcc
     3601 tggatgccaa ccgtctggag caggtgctgt tcaacctgcc ggccggcgac tgggccaagg
     3661 gcgagcgcgg tatcgcctgc caccccgacc gcgttgaaga gttccgcgcc ggtgttgacc
     3721 aggccatcgc ctacgccaag gtgctgggca acaagcagat caactgcctg gccggtatcc
     3781 gtccgcaggg tctggattgc gccaccgtcg aggcgacctt tgtcgacaac ctcaagtttg
     3841 ccgccggtaa gctcgaagcc gcaggcatta agctggtcat ggaaatgatc aacacccgcg
     3901 acatcccggg cttctatctg aacaccacca agcaggcgct ggctattcgc gacaaggtcg
     3961 ccagcagcaa cctgtacctg caatacgaca tctaccacat gcaaatcatg gaaggtgacc
     4021 tggcgcgtac cgtcgagacc aatctgtcag tgatcaacca tgtgcagttg gcggacaacc
     4081 cgggccgcaa cgagccgggc actggcgaga tcaactaccg cttcctcttc gagcacctgg
     4141 accgcatcgg ctaccagggc tggatcggct gtgaatacaa gccggcaacc accaccgctg
     4201 ccggcctcgg ctggatgaaa gcgcacaacg caatctgaat cagaggatat ttctcatggc
     4261 aaaaatcgga tttatcggca ccggcatcat gggcaagcct atggcgcaga acctgcaaaa
     4321 agccgggcac accctgttct tct
//
LOCUS       DASRUM010000083         4969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_208484, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000083 DASRUM010000000
VERSION     DASRUM010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208484"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(413..2164)
                     /gene="arsA"
                     /locus_tag="VFF22_05715"
     CDS             complement(413..2164)
                     /gene="arsA"
                     /locus_tag="VFF22_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003118375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HZX16346.1"
                     /translation="MRFIDNAPRFLFFTGKGGVGKTSIACATALELTRQGKRVLLVST
                     DPASNVGQVFGLEIGNHITAVPAVENLAALEIDPQGAAQAYRDRIVGPARGVMPDDVV
                     KGMEEQLSGACTTEIAAFDEFTSLLVNDKLKTRFDHIIFDTAPTGHTIRLLQLPGAWS
                     GFLEAGQGDASCLGPLAGLDKQRDQYREAVEALSDPAKTRLVLVARAQAATLREAART
                     REELAAIGLQEQYLVINGVLPASEAQKDVLAAAVYKREQASVAAMPPALRALPLDQLP
                     LKAFNLVGLNALRQLLVEDDVGTQAPACAPVELDAPRLKSLIDEIADNGHGLIMVMGK
                     GGVGKTTLAAAVAVELARRGLPVHLTTSDPAAHLAETLDGTLEQLTLSRIDPHVETQR
                     YRDHVMATKGAVLDEQARALLAEDLRSPCTEEIAVFQAFSRIIREAGKKFVVMDTAPT
                     GHTLLLLDATGAYHRDIARQMSGNNMRFSTPMMQLQDPKQTKVLLVTLAETTPVLEAA
                     SLQADLRRAGIEPWAWVINNSVAAAKTESPLLRKRAANELRDIEHVATEHAQRYAVIP
                     LMPEEPVGVELLLELST"
     gene            complement(2176..2363)
                     /locus_tag="VFF22_05720"
                     /pseudo
     CDS             complement(2176..2363)
                     /locus_tag="VFF22_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721778.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
     gene            2495..3277
                     /locus_tag="VFF22_05725"
     CDS             2495..3277
                     /locus_tag="VFF22_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003118377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZX16347.1"
                     /translation="MSQKKRVLFLCVANSSRSQMAEALLRHTDSEHFEVYSAGSVPTE
                     IDIRARNALEQLGVPTGGLYSKSIEQVAGAPFDYVITLCDKSALECQALPGAGEYIAW
                     NFEDPATSAQKDSYRKTLHEIHERIKMFVLIKSKPAKDASTLHAMSPTDVFKCLADET
                     RARIALLVTLEQELCVCELTSALEEAQPKISRHLALLRGCGLLEDRRKGQWVYYRLHP
                     QLPEWVTNILKQTLDSNAEWLREDVQRLWQMRDRPGRKPACG"
     gene            3301..3771
                     /locus_tag="VFF22_05730"
     CDS             3301..3771
                     /locus_tag="VFF22_05730"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019752755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HZX16348.1"
                     /translation="MKILFLCTANSCRSILSEAIFNTMAPEGMRAFSAGSHPKGEVNP
                     LALQALLNAGFDVEGLYSKSTDAHRVLVPDIVITVCDKAAGEACPVFFGPAIRSHWGL
                     SDPSEVNGSEAEINAAFAATIQQIKKRVSAFIALPFAQLDSDALKAELNRIGSF"
     gene            3896..4396
                     /locus_tag="VFF22_05735"
     CDS             3896..4396
                     /locus_tag="VFF22_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclin-dependent kinase inhibitor 3 family
                     protein"
                     /protein_id="HZX16349.1"
                     /translation="MNHPYDLLSIPGIQGKLIFTPCPGTKDSSLAEALTTLKQAGASA
                     VISLMPASELAANGAEGIGKQCQAQGMAWFHLPVADEQVPLEDFGQSWNASRQSILER
                     LNAGQDIAIHCKGGSGRTGLIAARIMIEVGISRADAIALVQALRPKAIQHPAHLNWIT
                     QFGAAH"
     gene            4420..>4969
                     /locus_tag="VFF22_05740"
     CDS             4420..>4969
                     /locus_tag="VFF22_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012316902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HZX16350.1"
                     /translation="MAIKIGINGFGRIGRLALRASWDWPEFEFVQINDPAGDAATHAH
                     LLNFDSVHGRWQQEACSDGDYVVIGGKRIKVTANKSIADTDWSGCDLVLEASGKMKTV
                     AVLQAYLTQGVKRVVVCAPVKEQGALNVVMGVNQHLFNPAEHRIVTAASCTTNCLAPV
                     VKVIHENLGIRHGSITTIHDLTN"
BASE COUNT         1062 a         1492 c         1399 g         1016 t
ORIGIN      
        1 cggtgcagaa gttcctctac tgctattgcc gaaaacttaa ttgattccgt ttccatgatt
       61 cttcgcgggc caaaagtggg cgaccttggc taggtatctt tagcgagcaa tgtgtctgct
      121 cctggccgca agccgccggc ctggcaaacc atgccgcagc tgccgtgctg cgtaacccgg
      181 caaggttgag gatggccgtc tctgggtttt ccgcgaaaac tctcaggagc tgaaagactt
      241 caaaaccacc ggtgagccag ccaagcagtt cactgatatc agcaccggcc cgaatggcat
      301 gacggtcaaa gctgctaacg agaaaaccct caaggactac cttgagatca tcaagaagta
      361 atagctttca agcaaaaagg cccgcgattg cgggcctttt cgtggctgcg aattacgtgc
      421 tgagttccag taaaagctca acccctactg gttcctcggg cattagcgga atgaccgcat
      481 agcgttgcgc gtgttcggtt gccacatgct caatatccct cagctcattg gcagcccgct
      541 tgcgtaatag cggtgattca gtctttgcgg cagccacact gttgttgatc acccatgccc
      601 acggttcaat gcctgcacgg cgcaaatcag cttgcagact cgcggcctcc agcactggag
      661 tggtttcagc cagggtcacc agcagcacct tggtctgttt tggatcctgg agctgcatca
      721 tcggggtaga aaaacgcatg ttattgcccg acatctgccg agcaatgtca cggtgatatg
      781 ccccggtggc atccagtagt agcaaggtat gccctgtggg agcggtgtcc atcaccacga
      841 atttcttgcc ggcttcacgg atgattcgcg aaaatgcctg gaagacggcg atctcctcgg
      901 tgcagggtga gcgcagatcc tcggccagca gtgcccgagc ctgctcatcc agtaccgccc
      961 ccttggttgc catcacatga tcacgatagc gctgtgtctc gacatgcggg tcaatccggc
     1021 tcagcgtcag ttgctcaagt gtgccatcca gtgtctcggc aaggtgcgcg gcagggtcag
     1081 aggtggtcag gtgtacaggc aacccccgac gagccagttc gacggcaacc gctgcggcga
     1141 gggtggtttt tccgacaccg cctttaccca tcaccatgat caggccatga ccattgtcgg
     1201 cgatctcgtc gatcagggat ttgagtcgcg gcgcgtccaa ttcaaccggc gcgcaggccg
     1261 gtgcctgtgt gcctacatcg tcctcaacca ggagttgtcg cagggcgttc aatcccacca
     1321 ggttgaatgc cttcaacggc agctgatcga gcggcagtgc gcgcagcgct ggcggcatag
     1381 cggcgaccga agcctgttca cgcttataga ctgcggcggc caggacatcc ttctgcgcct
     1441 cgctcgccgg tagcacgccg ttgatcacca ggtactgctc ctgcaagccg atggctgcaa
     1501 gctcctcgcg tgtacgggcc gcctcgcgca gggtggcggc ctgcgccctg gcaaccagaa
     1561 ccaggcgggt cttggctgga tcagaaagcg cttctactgc ctcgcgatac tggtcacgtt
     1621 gtttgtccaa accggccagc gggccaaggc acgatgcatc accttgcccc gcctccagga
     1681 atccactcca ggcgccgggc agttgcagca gccgaatcgt gtgcccagtc ggggctgtat
     1741 cgaaaataat gtggtcgaag cgggtcttca acttatcgtt gaccagcagt gaagtgaatt
     1801 cgtcgaaggc tgcaatctcg gtcgtgcagg cccctgacaa ctgctcttcc atgcccttga
     1861 ctacgtcatc gggcatgacc cctctcgccg ggcccacaat gcgatcccga taagcctggg
     1921 ctgcaccctg agggtcgatc tccagggccg ccagattttc cacagcgggc acggcagtaa
     1981 tgtggttgcc gatctccagg ccgaatacct gccccacatt ggaggccggg tcagtactta
     2041 ccagaaggac acgttttccc tgacgggtca gttcaagcgc ggtagcgcag gcaatcgagg
     2101 ttttcccgac cccgccctta ccggtgaaga acaggaagcg tggtgcattg tcgataaagc
     2161 gcatgctgac accccttagt gattgttgag gctgcactcg taccagttac gtgtgctgtt
     2221 caccatgcgt accagcaaga gcatcacggg cacctcgatg agcacgccga ccacggttgc
     2281 caaggctgcc cctgagttga accgtacaac acaatcgcca cggcaacggc tagctcgaag
     2341 aagttggagg ccccgatcat ggcgacttca cacgcattta tcatctttgc tgcctatcct
     2401 cgtttacttg gcctctcagc gctcaatata ttcactttac tgcatattcg aaaaataata
     2461 tatttgctcg tatgcatctt tgatggggat caatatgagc cagaaaaagc gcgtcctgtt
     2521 tctctgcgtg gcgaactcat cacgctcgca aatggcagaa gctcttctgc gccacaccga
     2581 ttcggaacat tttgaagtct acagcgcagg ctccgtgccc actgaaatcg acatccgcgc
     2641 gcgcaacgcg ctggagcagc ttggtgttcc cactggcggg ctttacagca agtccatcga
     2701 gcaggtcgct ggcgcgccct tcgactatgt gatcaccttg tgtgacaagt ccgccctgga
     2761 gtgccaggcg ctgcctgggg ccggtgagta catcgcctgg aacttcgaag acccggcgac
     2821 aagcgctcag aaagattcct atcgcaagac cctgcatgag attcatgagc gcataaagat
     2881 gtttgttctg atcaagagca agccggctaa agacgcttcc accctgcatg ccatgtcgcc
     2941 aaccgacgta ttcaaatgcc tggccgatga gactcgggcg cgtatcgctt tgctcgttac
     3001 cctggagcaa gagctttgtg tctgtgaact gacctccgcg cttgaagagg cccaacccaa
     3061 gatatcccga cacctggccc tcttgcgtgg ctgcggtctg ctggaggatc ggcgcaaggg
     3121 ccagtgggtg tactaccgtt tgcacccgca gctgcctgag tgggtgacca atattctgaa
     3181 gcagaccctg gatagcaatg ccgaatggtt gcgcgaggac gtgcaacgcc tctggcaaat
     3241 gcgtgatcgc ccagggcgca agccggcctg cggctgagtg caagtcacgg ggtgctgatt
     3301 atgaaaatac tgtttctctg cacggccaac agctgccgta gcatcctgtc ggaagccatc
     3361 ttcaacacca tggctcctga gggcatgcgg gcctttagcg cgggcagtca ccccaagggg
     3421 gaagtcaacc cgttggcctt gcaggcactg cttaatgccg ggttcgatgt tgaagggcta
     3481 tacagcaaat caaccgatgc ccatcgggtt ctcgtccctg acatcgtcat taccgtctgc
     3541 gacaaagcag ccggagaggc ctgtcctgtt ttcttcgggc cggcgattcg tagtcactgg
     3601 ggtctgagtg acccatccga agtcaacggg tctgaggcag aaatcaacgc cgcatttgca
     3661 gccaccatcc agcagattaa gaagcgagtg agcgcattta tcgcgctgcc ctttgctcaa
     3721 ctcgatagcg atgcgctaaa ggcagagctc aacagaatcg gctcgttcta gcccaagagg
     3781 tacagaccca tgatcgacaa cctgtacagc gccgctattc ggagcgtaaa gagtccgctg
     3841 tagaactctc caagcgcgtc aaccaacgcg ccatctgact gtcaggaatc acccaatgaa
     3901 tcacccatac gacctgctca gcatccccgg catccaaggc aaactgatct tcacgccatg
     3961 cccgggcacc aaagacagca gccttgcaga ggcgctgacc acgctcaaac aggccggcgc
     4021 ctctgccgtg atcagcctga tgccggcaag cgaattggcc gccaacggag ccgagggcat
     4081 cggcaagcaa tgccaggcgc aaggcatggc ctggttccac ttgccggttg ccgatgagca
     4141 agtaccgctt gaggatttcg gccagagttg gaacgcgtcc aggcagagca tccttgagcg
     4201 actcaatgca ggccaggaca tcgccatcca ctgcaagggc ggttcgggac gtaccgggct
     4261 gattgcggcg cgaatcatga tcgaggtcgg catttcacgc gccgatgcca ttgccctggt
     4321 gcaggccctg cgccccaagg ctattcaaca ccctgcccac ctcaactgga tcacccagtt
     4381 cggtgctgcc cactaactcc aacagcgaaa acgaagaata tggctatcaa aattggtatc
     4441 aacggtttcg gtcgtatcgg tcgcctggcg ctgcgcgcct cctgggactg gcccgagttc
     4501 gagttcgtgc agatcaacga tccggcaggc gacgcggcca cccacgcgca cctgctcaac
     4561 ttcgattcgg tgcatggtcg ctggcagcaa gaagcctgca gtgacggcga ttacgtggtg
     4621 atcggcggca agcgcatcaa ggtgacggcc aataaaagca ttgccgatac cgactggtcg
     4681 ggctgcgatc tggtgctcga agccagcggc aagatgaaga ccgtggcggt attgcaggct
     4741 tacctgacgc aaggggttaa gcgggtagtg gtctgcgcgc cggtaaaaga gcagggtgca
     4801 ctgaacgtgg tgatgggcgt taaccagcac ctgttcaatc cggcggagca ccgcatcgtc
     4861 accgccgcct cctgtaccac caactgcctg gcaccggtgg tcaaggtgat ccacgagaac
     4921 ctcggtatcc gtcacggctc gatcaccacc atccatgacc tgaccaata
//
LOCUS       DASRUM010000084         7353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1550882, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000084 DASRUM010000000
VERSION     DASRUM010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7353
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550882"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..338
                     /locus_tag="VFF22_05745"
     CDS             213..338
                     /locus_tag="VFF22_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014861347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HZX16351.1"
                     /translation="MKRLFTAFVAMLAVSALLAGCGQKGPLYLPEESQPAPAQTE"
     gene            348..1595
                     /gene="lysA"
                     /locus_tag="VFF22_05750"
     CDS             348..1595
                     /gene="lysA"
                     /locus_tag="VFF22_05750"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZX16352.1"
                     /translation="MNAFNYRDGALFAEGVALSAIAQRFGTPTYVYSRAHIEAQYRAY
                     ADALQGMPHLVCFAVKANSNIGVLNVLARLGAGFDIVSRGELERVLSAGGEPSKIVFS
                     GVGKSRDDMRRALEVGVHCFNVESTDELERLQIVAAELGKTAAISLRVNPDVDAGTHP
                     YISTGLKENKFGIDIEQAPAVYARAAALPNLQVVGVDCHIGSQLTTLPPFLDALDRLL
                     ALIDQLAADGIRIKHLDLGGGLGVQYRDEQPPLAGDYIKAVRERIAGRDLALVFEPGR
                     SIVANAGVLLTQVEYLKHTEHKDFAIVDAAMNDLIRPALYQAWMEVVAVQPRDGAPRN
                     YDIVGPICETGDFLAKDRQLALAEGDLLAVRSAGAYGFVMSSNYNTRGRAAEVMVDGD
                     QAFEVRRRETLSELFAGESRLPE"
     gene            1601..2431
                     /gene="dapF"
                     /locus_tag="VFF22_05755"
     CDS             1601..2431
                     /gene="dapF"
                     /locus_tag="VFF22_05755"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HZX16353.1"
                     /translation="MLLRFTKMHGLGNDFMVLDLVSQHAHIQPKNAKAWGDRHTGVGF
                     DQLLLVEPPSNPDVDFRYRIFNSDGSEVEQCGNGARCFARFVLDKRLTVKKQMRVETK
                     GGIIELDVRNDGQISVNMGAPRLVPADIPFQAEQQALSYSVAVDGQTIELAAVSMGNP
                     HAVLRVDSVDNAPVHSLGPKLEHHSRFPQRVNVGFLQVVDRKQAKLRVWERGAGETQA
                     CGTGACAAAVAAISQGWMDSPVQLELPGGKLSIEWAGPGQPVMMTGPAVRVYEGQVRL
                     "
     gene            2428..3126
                     /locus_tag="VFF22_05760"
     CDS             2428..3126
                     /locus_tag="VFF22_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HZX16354.1"
                     /translation="MTDKQDSPKPLDSDTVAAYLRLHPEFFIDHDELIPELRIPHQRG
                     DTVSLVERQVKLLRERNIEMRHRLSQLMDVARDNDRLFDKTRRLVLDLLDASSLEEVV
                     SCVEDSLRREFQVPFVSLILFSDAPLPVGRTVSSAEAHQAIGGLLAGGKTICGVLREH
                     ELTFLFGSEEGAQVGSAAVVSISYQGLHGVLAIGSADPQHYKSSLGTLFLGYIAEVLA
                     RVVPGFATPLRSVR"
     gene            3183..4079
                     /gene="xerC"
                     /locus_tag="VFF22_05765"
     CDS             3183..4079
                     /gene="xerC"
                     /locus_tag="VFF22_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HZX16355.1"
                     /translation="MQDLLDAYLEHLRSERQLSSHTLIGYRHDLRTLLAFCTQEQIDS
                     WSALDTARLRRMVGRLNMQGQSSRSLARLLSATRGLYRYLIREGHCQSDPASGLTPPK
                     GERRLPKTLDADRTAQLLDGALEDDFIARRDQAMLELFYSSGLRLSELVGLNLDGLDL
                     PAGLVRVIGKGNKTRELPLGRMARQAMEQWLPLRALANPADGAVFISQQGRRIGPRAV
                     QLRVRQAGVRELGQHLHPHMLRHSFASHMLESSQDLRAVQELLGHADIATTQVYTHLD
                     FQHLATVYDQAHPRAKRKGSTE"
     gene            4076..4771
                     /locus_tag="VFF22_05770"
     CDS             4076..4771
                     /locus_tag="VFF22_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZX16356.1"
                     /translation="MSIQLITFDLDDTLWDVTPVMQDAEAALRNWLAMHAPRLGAVPV
                     EHLWSIRATLLQAEPMLKHRLSELRRRILLHALTDAGYPHSDAQALAEAGFQVFLAAR
                     HQVELFAEVHPTLEALANRFQLGVITNGNADVRRLGLADYFQFALCAEELGVGKPDPK
                     PFQEALKRAGIAAEHAVHIGDHPSDDIAGAQAAGMRAIWFNPQGKAWEADNTPNGQIR
                     SLAELPTLLHSWS"
     gene            complement(5036..5365)
                     /gene="sutA"
                     /locus_tag="VFF22_05775"
     CDS             complement(5036..5365)
                     /gene="sutA"
                     /locus_tag="VFF22_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SutA"
                     /protein_id="HZX16357.1"
                     /translation="MSDEDLEHDELEGADEDEGEELAAADDGDSSDDGDGDDEVVPAS
                     KSKKAKAAVDVEELPSIEAKQKERDALARAMEEFLSRGGKVQEVEPNVVSDPPKKPDS
                     KYGSRPI"
     gene            complement(5624..6874)
                     /locus_tag="VFF22_05780"
     CDS             complement(5624..6874)
                     /locus_tag="VFF22_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZX16358.1"
                     /translation="MDNTALTALQYGFDTFYFLICGALVMWMAAGFAMLESGLVRAKN
                     TTEILTKNIALYAVASIMYLVIGYHIMYSSPEGGILPSLGFLIGDENSVESVLAGGDD
                     APYYSARSDFFFQVVFAATCMSIVSGAVAERMKLFAFLAFAVVMTGFIYPVQGFWKWG
                     SGFLNEAGFLDFAGSGVVHMAGATAALAGVLLLGARKGKYGPNGQVNAIPGANLPMAA
                     LGTFILWMGWFGFNGGSQLKMSTIEDANAVANVFVNTNMAAAGGLIAALIVARILFGK
                     ADLTMALNGALAGLVAITAEPLTPTALQATLIGGVGGVLVVFSILGLDKLKLDDPVGA
                     ISVHGVVGIWGLLAVCLTNSDATLGAQLLGIGSIFAWVFVASLIVWSIIKLVIGLRVT
                     EEEEYEGVDIAECGMEAYPEFTKR"
     gene            complement(6953..>7353)
                     /locus_tag="VFF22_05785"
     CDS             complement(6953..>7353)
                     /locus_tag="VFF22_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HZX16359.1"
                     /translation="TPASGTAGPMGALAIGLASGAICFFCATSLKRKLGYDDSLDVFG
                     VHAVGGIVGAILTWAFAAPALGGFGTVEDIPAQLWIQFEGVAFTVVYTAVVTFVILKV
                     IDLVMGLRVSEEEETIGLDLALHNERGYNL"
BASE COUNT         1465 a         2382 c         2174 g         1332 t
ORIGIN      
        1 tggaggactt ggcaagggaa caccattacc tgcgtcctcc gcctagcctg gcgagatttt
       61 tcgcggcaac gcggctcgcg acgaaatggg aacagaccct agcgccaaag atacaggcaa
      121 agccccgccc gacgggaacc ccggcacata ggcaaggcgc cggggggtcg gtatactccg
      181 gcgcaatcat gctttattac aaggattcca ccatgaagcg gttatttact gcctttgtcg
      241 cgatgcttgc cgtcagcgcc ctgctcgctg gctgcggcca gaaaggcccg ctgtacctgc
      301 cggaagaaag ccagccagcg cctgcgcaaa ccgaataagg acagcttatg aacgccttca
      361 actaccgcga cggtgcgctg ttcgcggaag gcgtggcgtt gtccgccatc gcccaacgct
      421 tcggtacgcc gacctacgtc tactcgcgtg cgcacatcga ggcgcagtac cgcgcctatg
      481 ccgatgccct gcagggcatg ccgcatctgg tgtgttttgc ggttaaagcc aactccaata
      541 tcggcgtgct gaatgtgctg gcgcgcctcg gcgctggctt cgacatcgtc tcccgtggcg
      601 agctggagcg cgtactcagc gccggtggcg agccgagcaa gatcgtcttc tccggcgttg
      661 gcaagagtcg tgacgacatg cgccgcgccc tggaagtggg cgtgcactgc ttcaacgtcg
      721 agtccaccga cgagctggaa cgcctgcaaa tcgtcgccgc cgagctgggc aagactgcgg
      781 cgatctcgct gcgggtcaac ccggacgtcg acgctggcac ccacccgtac atttccacgg
      841 gccttaaaga aaataaattc ggcatcgata tcgagcaggc tccggccgtt tatgcccgcg
      901 ccgccgcgct gccaaacctg caggtggtgg gtgtcgattg ccatatcggc tcgcagctga
      961 ctacgttgcc gccgtttctc gatgccctcg accgtttgct cgcgctgatt gatcagctcg
     1021 ccgcagacgg catccgcatc aagcacctgg atctgggtgg cggccttggc gtgcagtacc
     1081 gtgacgaaca accgccgctg gccggcgatt acatcaaagc cgtgcgcgag cgcattgccg
     1141 gccgcgacct ggccctggtg ttcgagccgg gccgttcgat tgtcgccaac gccggcgtgc
     1201 tgctgaccca ggtcgaatac ctcaagcaca ccgaacacaa ggacttcgcc atcgtcgacg
     1261 cagcgatgaa cgacctgatc cgcccggccc tctaccaggc atggatggag gtggtggccg
     1321 tgcaaccgcg cgatggcgcg ccgcgcaact acgacatcgt cggaccaatt tgcgagaccg
     1381 gcgatttcct cgccaaagac cgccagctgg ccctggccga aggcgatctg ctggccgtgc
     1441 gctcggccgg tgcctacggt tttgtcatga gctccaacta caacacccgc ggccgcgccg
     1501 ccgaggtgat ggtggatggc gatcaagcct tcgaagtgcg tcgccgtgag acgctcagcg
     1561 aactctttgc gggcgaaagc cgcctgccgg agtagtggcg atgttattgc gctttaccaa
     1621 gatgcacggc ctcggcaatg actttatggt cctcgatctg gtcagccagc acgcgcatat
     1681 ccagccgaag aacgccaaag cctggggcga ccgccacacc ggcgtgggct tcgatcaact
     1741 gctactggtt gagccgccaa gcaacccgga cgtggatttc cgctaccgca tcttcaattc
     1801 cgacggctcg gaagtggagc agtgcggcaa cggcgcgcgc tgcttcgccc gcttcgtgct
     1861 ggacaagcgc ctgacggtga agaagcagat gcgcgtggaa accaagggcg gcatcatcga
     1921 gctggatgtg cgcaacgacg gacagatcag cgtcaacatg ggcgcaccgc gcctggtgcc
     1981 agccgatatc ccgttccagg ccgaacagca ggcgctgagc tacagcgttg cagtcgacgg
     2041 ccagaccatt gagctggccg ccgtgtccat gggcaacccg catgccgtgc tgcgcgtcga
     2101 cagcgtcgac aacgcgccgg tgcacagcct ggggccaaaa ctcgaacacc attcgcgctt
     2161 cccgcagcga gtcaatgtcg gcttcctgca agtagtcgac cgcaagcagg ccaagttgcg
     2221 cgtttgggaa cgcggcgccg gggaaaccca ggcctgcggt accggcgctt gcgcagcggc
     2281 agtggcggcg atcagccagg gctggatgga ttcaccggtg cagctcgaac tgcccggcgg
     2341 caaactgagc atcgagtggg caggccctgg ccagcccgtt atgatgaccg gacccgcagt
     2401 acgtgtgtac gaaggacagg ttcgtttatg accgacaagc aggattcacc caagccactg
     2461 gattcggaca cggttgccgc ctatctgcgc ctgcatccgg aattctttat cgaccatgac
     2521 gagttgatcc ccgagctgcg cattccgcac cagcgcggcg ataccgtgtc gctggtcgag
     2581 cgccaggtga aactgctgcg cgagcgcaat atcgaaatgc gtcaccgcct ctcgcaattg
     2641 atggacgtag cccgcgacaa cgaccgcctg ttcgacaaga cccgccgcct ggtgctcgat
     2701 ctactcgatg ccagcagtct ggaagaagtg gtcagctgcg tcgaagacag cctacgccgc
     2761 gaatttcagg tgcccttcgt cagcctgatt ctgttcagcg acgcaccgct gccggtcggc
     2821 cgtacagtca gcagcgccga ggcacaccag gcaatcggcg gcctgctggc cggtggcaaa
     2881 accatctgcg gcgtactgcg cgagcatgag ctgaccttcc tgttcggcag cgaagaaggt
     2941 gcgcaagtcg gctcggcggc agtggtgagc atttcctacc agggcctgca tggcgtactg
     3001 gcgattggca gcgccgaccc gcagcattac aaaagctcgc tgggcacgct gttcctcggt
     3061 tatatcgccg aagtgctggc acgcgtcgta ccgggctttg ccacaccact gcgctcggta
     3121 cgctaatagc tctaaacatc tagcactcac accgcaccga ccgcgcaccg cagggagctt
     3181 ccatgcaaga cctcctggac gcctacctcg aacacctgcg cagcgagcgc cagctttctt
     3241 cccataccct gattggctac cgtcacgatc tgagaacact gctggcgttc tgcacccagg
     3301 agcagatcga cagctggagc gcgctcgata ctgcacgttt gcgccgcatg gtcggccgcc
     3361 tgaatatgca gggccagtcc agccgcagcc tggcacgtct gctttcggcc acccgcggcc
     3421 tgtaccgtta cctgattcgc gagggtcact gccagagcga tccagccagc ggcctgacgc
     3481 cacccaaggg cgagcgtcgc ctgccgaaaa ccctggatgc cgaccgtact gcgcagctgc
     3541 tcgatggcgc gctggaagac gactttatcg cccgccgcga ccaggccatg cttgagctgt
     3601 tctattcatc aggtctgcgc ctgtccgagc tggtgggcct caacctggat ggtctcgacc
     3661 tgcctgcagg gttggtacgg gtgatcggta aaggcaacaa aacccgagaa ttgccgctcg
     3721 ggcgcatggc gcgccaggcc atggaacagt ggctgccgct gcgtgcactg gccaacccag
     3781 ctgatggtgc ggtgtttatc agtcagcaag gccggcgtat cggcccgcgc gcggtgcaac
     3841 tgcgcgtgcg ccaggccggt gtgcgcgagc tgggccagca cctgcacccg catatgctgc
     3901 gccacagttt cgccagccat atgctcgaat cttcccagga cctgcgcgcc gtgcaggaac
     3961 tgctcggcca tgccgacatc gccaccaccc aggtttacac ccacctggat ttccagcacc
     4021 tggccacggt ctatgaccag gcacacccca gggccaaacg caaaggcagc acagaatgag
     4081 cattcagctg attaccttcg acctcgacga caccctgtgg gatgtcaccc cggtgatgca
     4141 ggacgccgaa gcggcactgc gcaactggct ggccatgcat gcgccgcgcc tgggtgcggt
     4201 gccggtggaa cacctgtggt cgattcgcgc caccttgctg caggccgagc cgatgctcaa
     4261 gcatcgcctc agcgaacttc gccggcgcat cctgctgcat gccctgacag acgccggtta
     4321 cccgcacagc gatgcgcagg cgctggccga agccggcttt caggtatttc ttgccgcgcg
     4381 ccatcaggtc gaactgttcg ccgaggtgca tccaaccctt gaagccttgg ccaatcgctt
     4441 ccagctcggg gtcatcacca acggcaatgc cgacgtgcgc cgtctgggcc tggccgacta
     4501 tttccagttc gccttgtgcg ccgaggagct gggtgtcggc aaacccgatc ccaagccgtt
     4561 tcaggaagcc ctcaagcgcg ccgggatagc cgccgagcac gccgtgcata tcggcgatca
     4621 ccccagcgac gacatcgccg gagcccaggc tgccggtatg cgagcgatct ggttcaaccc
     4681 ccaaggcaaa gcctgggaag ccgacaacac gccgaatggg cagatccgca gcctggccga
     4741 gctgccaacg ttgctgcata gctggagctg agcggcgagc atattagggc gtcaggcgta
     4801 ggctgggtca ggccgcgcag gttagcggcc accccattcc cgcgcgagcc acaaactcag
     4861 gtgccgcgta acccaacaga gtatccgcag gcacgctgcg tgatgggtta cgccgccccc
     4921 aacatgcggc attgccaaag atcgccacgg cggctaaccc atcctacgat ccgaccagcc
     4981 acaaaaaagc ccgccggctt atggcctgcg ggcttttttg ttttacggca ggcccttaga
     5041 tagggcggct gccgtacttg ctgtccggct tcttgggcgg atcggagacc acattcggct
     5101 cgacttcctg caccttgcca ccacgggaga gaaactcttc catggcgcgg gccagagcat
     5161 cacgctcttt ctgcttggct tcgatgctcg gcagttcttc tacgtcgacg gcggctttgg
     5221 cttttttgct tttgctggca ggtacgactt cgtcgtcacc atcgccatca tcgctgctgt
     5281 cgccatcgtc ggcggccgcc agctcctcgc cttcgtcttc gtcagcgcct tctaattcgt
     5341 cgtgttccag atcttcgtcg ctcatgttcc aacctcatga cttgcgaaaa gcagagtagt
     5401 tatagcccag ctgcgtcagc tttctaacgt cgctgataaa aattccagtg gccttgcctt
     5461 gggctgagcg ctgcgcatcg gcgaactgtg ctcaacggcg cgcattctag ggtctgttgc
     5521 cgctccatca caaccgcgca catgctgaaa aaccacaacc ctttatttgc tttcggaaaa
     5581 aacaaagggc gcccttaggc gcccttgttg aaagcagagg aaatcaacgc ttggtgaatt
     5641 ccggataggc ttccataccg cattcggcga tgtccacgcc ttcgtactct tcttcctcgg
     5701 taacacgcag gccgatcacc agtttgatga tggaccagac gatcagactg gcaacgaaga
     5761 cccaggcgaa gatcgaaccg atacccagca gctgtgcgcc gagagtagcg tcgctattgg
     5821 tcaggcagac agccagcagg ccccagatac cgaccacacc gtgaacggag atggcaccga
     5881 ctggatcgtc gagcttgagc ttgtccagac caaggatcga gaacaccacc agcacgccac
     5941 ccacgccacc gatcagggtt gcttgcaggg cagtcggggt cagcggctcg gcagtgatgg
     6001 ccaccaggcc agccagggca ccgttgaggg ccatggtcag gtcagccttg ccgaacagga
     6061 tgcgtgccac gatcagcgcc gcgatcaggc caccggcagc cgccatattg gtgttcacaa
     6121 acacgttggc cacagcgttg gcgtcttcga tggtgctcat cttcagctgc gagccaccgt
     6181 tgaagccgaa ccagcccatc cacaggataa aggtacccag tgctgccatc ggtaggttgg
     6241 cacccggaat ggcgttgacc tgaccattcg ggccgtactt gcctttacgc gcgccgagca
     6301 gcagtacgcc agccagagca gcagtagcgc cagccatgtg caccacgcca gaaccggcga
     6361 agtcgaggaa gcccgcttcg ttgaggaagc ccgaacccca tttccagaag ccctgtaccg
     6421 ggtagatgaa accggtcatg accacggcga aagccaggaa ggcaaacagc ttcatacgct
     6481 cagccacggc accggagacg atcgacatac aggtcgcggc gaacaccacc tggaagaaga
     6541 agtccgaacg tgccgagtag taaggtgcat cgtcgccgcc ggccagaacc gactcaacgc
     6601 tgttctcatc accaatcagg aaacccaggc ttggcaggat gccgccttcc gggctggagt
     6661 acatgatgtg gtagccgatc accaggtaca tgatgctcgc cactgcgtac agggcgatgt
     6721 tcttggtgag aatttcagtg gtgttcttgg ctcgcaccaa accggattcg agcatggcaa
     6781 aacccgctgc catccacata accagggcgc cgcaaatcaa aaagtagaag gtatcaaaac
     6841 cgtactgcag tgcagtcaat gctgtgttgt ccatggttca cctcttgccg tgcgctttat
     6901 agcgctattc ggttaaggcg tccggggttg gctccggacg cgctccgtgt gcttacaggt
     6961 tgtagccgcg ctcgttatgc agggcgagat cgagaccgat ggtctcttcc tcttcgctga
     7021 cacgcaggcc cattactagg tcgatcactt tcagaatcac gaaggtcacc acagcggtgt
     7081 acaccactgt gaaggccacc ccctcgaact ggatccacaa ctgcgccgga atatcttcca
     7141 ccgtgccgaa gccacccaag gccggtgcgg cgaacgccca ggtgagaatg gcgccgacaa
     7201 tgccgcccac ggcgtgcacg ccgaacacgt cgagggaatc gtcataaccc agcttgcgtt
     7261 tcaggctggt ggcgcagaag aagcagatcg ccccacttgc cagaccaatc gccagagcac
     7321 ccatcggccc cgcggtaccg gacgccggag tga
//
LOCUS       DASRUM010000085         9234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_381666, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000085 DASRUM010000000
VERSION     DASRUM010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9234
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381666"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..2257
                     /locus_tag="VFF22_05790"
     CDS             53..2257
                     /locus_tag="VFF22_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011534436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC domain/YcaO domain-containing protein"
                     /protein_id="HZX16360.1"
                     /translation="MEIKVKFLDNLRLEAKFDDFTVVADQPIRYKGDGSAPGPFDYFL
                     ASSALCAAYFVKLYCDTRNIPTDNIRLSQNNIVDPENRYQQIFKIQVELPADISEKDR
                     LGILRSIDRCTVKKVVQAGPEFVIEEVESLDADAQALLIPALTAGEGTRILGKDLPLE
                     ETIANMSGIMAKLGMKIEIASWRNIVPNVWSLHIRDAHSPMCFTNGKGATKEGALASA
                     LGEFIERLNCNFFYNDQFWGEEIANAAFVHYPDERWFKPGPKDALPAEILDEYCLAIY
                     NPDDELRGSHLYDTNSGNTLRGICSLPFVRQSDGEVVYFPSNLIENLYLSNGMSAGNT
                     LAEAQVQCLSEIFERAVKREILEGELCLPDVPQEVLAKYPAILAGIQGLEAQGFPVLV
                     KDASLGGEFPVMCVTLMNPRTGGVFASFGAHPSFEVALERSLTELLQGRSFEGLNDLP
                     PPTFESQALMEPNNFVEHFIDSSGVVSWRFFSAKADHEFVEWDFSSQGEHANTEEAAT
                     LFGILEAMGKEAYMAVYEHLGATACRILVPGYSEIYPVEDLIWDNTNKALLFRADILN
                     LHSLNDRGLKSLLRNLENSDVDDYTDITTLIGVEFDDNTVWGQLTILELKLLIHLALK
                     KFDDAKELVETFLQYNDNTVERGLFYQALNAALEVQMDDELEMADFEPNFRRMFGKER
                     MDAVLGSLDGSVRFHGLTPTNMQLEGLDRHLRLIESYKKLHAARARVAAVSG"
     gene            2392..2778
                     /locus_tag="VFF22_05795"
     CDS             2392..2778
                     /locus_tag="VFF22_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16361.1"
                     /translation="MSFVIGNFKWLMLVSGVLTASMFYGLFAPQAALESMFGASFTGQ
                     LASIVIRSWSALVGLMGVILIYGALNEKHRAFCAAIAATSKAIFVTLVLVYGQAFLSK
                     AAAAIIMDIVVISATLIFLLALRIKR"
     gene            complement(2799..3734)
                     /locus_tag="VFF22_05800"
     CDS             complement(2799..3734)
                     /locus_tag="VFF22_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020294549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HZX16362.1"
                     /translation="MTDPTSTDSAVYKTLLESTKAIPWKIDWASMTFAYIGPQIETLL
                     GWPQASWVSANDWAERMHPEDRDSVVAFCVSQSQAGTDHEADYRALTRDGDYVWIRDV
                     VHVLRNEAGEVESLIGFMFDISERKQTEQQLLQLQRQLEELSYLDGLTGVANRRMFDS
                     RLQVEWSNAQRTGLPLSLILLDIDYFKEYNDHYGHIQGDDCLKSVGQALSGAAVRPRD
                     LMARYGGEEFVLLLPETDAQAAAQVAERCRQLIREQNIQHAHSQVSPLLTLSLGVGTL
                     TPGPFDQPQAFLQTVDRLLYKAKHQGRNCAVLAST"
     gene            complement(3878..4771)
                     /locus_tag="VFF22_05805"
     CDS             complement(3878..4771)
                     /locus_tag="VFF22_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003253739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZX16363.1"
                     /translation="MKRFSFSNFMLWAGLTFIYLPMLILVIYSFNASRLVTVWGGWSL
                     KWYVGLLDNTQLMNSVMRSLEIATYTAIAAVALGTMAAFVLTRVTRFKGRTLFGGLVT
                     APLVMPEVITGLSLLLLFVAMAQLIGWPAERGVLTIWIAHTTFCSAYVAVVVSSRLRE
                     LDLSIEEAAMDLGAKPWKVFFLITIPMIAPSLAAGGMMSFALSLDDLVLASFVSGPGS
                     TTLPMEVFSAVRLGVKPEINAVASLILLAVSFATFLVWFFAHRAEEKRKKALQQAMDE
                     TTGAALMNGPDSRRDPSQVHA"
     gene            complement(4768..5649)
                     /locus_tag="VFF22_05810"
     CDS             complement(4768..5649)
                     /locus_tag="VFF22_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZX16364.1"
                     /translation="MPTGRHLVIGVPFLWLFLFFMLPFFIVLKISFAEADVAIPPYTD
                     VYTWVENKFTLVLNLGNYIFLSEDALYLAAYMGSLKMATISTLLCLVIGFPMAYAIAR
                     ADKDKQMVLLLLIMMPTWTAILIRVYAWMGILSNNGLLNGFLMWLGVINEPLQILNTN
                     LAVYIGIVYSYLPFMILPLFANLVKHDQSLLEAAADLGSSTYNSFWKITVPLAKNGII
                     AGCMLVFIPVVGEFVIPELLGGPETLMIGKVLWQEFFNNRDWPVASALAVVMLAILIV
                     PIILFNRNQAKEMEGRP"
     gene            complement(5676..6818)
                     /gene="potA"
                     /locus_tag="VFF22_05815"
     CDS             complement(5676..6818)
                     /gene="potA"
                     /locus_tag="VFF22_05815"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HZX16365.1"
                     /translation="MAVASSAYKKVLEGDQQPKEVLVKIERVTKKFDETIAVDDVSLT
                     INKGEIFALLGGSGSGKSTLLRMLAGFERPTEGRIILDGVDITDLPPYERPINMMFQS
                     YALFPHMSVADNIAFGLKQDKLPKAEIDARVEEMLKLVHMTQYAKRKPHQLSGGQRQR
                     VALARSLAKRPKLLLLDEPMGALDKKLRSQMQLELVEIIERVGVTCVMVTHDQEEAMT
                     MAQRIAIMHLGWIAQIGSPVDIYETPTSRLVCEFIGNVNLFEGQVVEDMEGHALIASP
                     DLERNIYVGHGVSTSVQDKSITYAIRPEKLLVTTEQPTCEHNWSRGTVHDIAYLGGHS
                     VFHVLLPSGKVVQSFVANAERRGARPTWDDEVFVWWEDDSGVALRS"
     gene            complement(6873..7964)
                     /locus_tag="VFF22_05820"
     CDS             complement(6873..7964)
                     /locus_tag="VFF22_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017245998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HZX16366.1"
                     /translation="MRRIPLLASLLAAAVSVSAAQAAEPVVKVYNWSDYVGETTLADF
                     QKATGIKALYDVFDSNETLEGKLLAGRSGYDVVVPSNHFLGKQIKAGAFQKLDRSQLT
                     NWENLDPVLLKQLEVNDPGNQYSVPYLWGTNGIGYNVAKIKEVLGVDEIDSWATVFEP
                     ENMQKLASCGVSFLDSADEMIPAVLNYMGLDPNTQSAEDYAKAEAKLLAVRPYVTYFH
                     SSKYIGDLANGDICVAVGFSGDILQAADRAEEAGKGIEVAYSIPQEGANLWFDMLAIP
                     VDASNPKEAHAFINYLLDPAAIAGVSDYVGYANPNIKAGELMDQEVRNDESVYPPQAV
                     LDKLFISAELPVKVQRLMTRSWTKVKSGK"
     gene            complement(8049..9146)
                     /locus_tag="VFF22_05825"
     CDS             complement(8049..9146)
                     /locus_tag="VFF22_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZX16367.1"
                     /translation="MNTFGKTLLALSLAGAVVSAAHADDKVLHVYNWSDYIAEDTLDN
                     FQKETGIKVVYDVFDSNETLEAKLLAGSSGYDIVVPSNPFLAKQIKAGVFQKLDKSKL
                     SNWENLDKSLLKALDPSDPGNQYSIPYMWGTIGIGYNVDKVKAALGEDAPVNSWDLVF
                     KPENIAKLKECGVSFLDSPTEILPAALNYLGFKPDSTDTGELKKAEELFLSIRSSTSY
                     FHSSKYIGDLANGNICVAIGYSGDLYQSKSRAEEAKNGVNISYSIPKEGAGSFFDMMA
                     IPADAKNVEAAHVFLNYLMKPDVMANITNFVQFPNGNAAATPLVDEVLRNDPGIYPDA
                     ATMAKIYTFPDLPANAQRAMNRSWTKIKSGR"
BASE COUNT         1833 a         2643 c         2865 g         1893 t
ORIGIN      
        1 aaaacccgga taatggccgc cattcgttta gcccgtattc tggtaatccc gcatggaaat
       61 caaggtcaag tttctcgaca acctccgact tgaagccaag ttcgatgact tcacggtggt
      121 ggctgaccag cccatccgct ataagggtga tggctcggcg ccggggccgt ttgattactt
      181 cctggcctcc tcggcgctgt gcgcggcgta ctttgtgaag ctgtattgcg atacccgcaa
      241 tatccccacc gacaacatcc gcctgtcgca gaacaacatc gttgacccgg aaaaccgcta
      301 ccagcagatc ttcaagattc aggtcgagct gccggcggat atctccgaga aggatcgcct
      361 gggcattctg cgttccatcg accgctgcac ggtgaagaag gtggtgcagg ccggacctga
      421 gttcgtgatc gaggaagtgg aaagcctcga tgccgatgcc caggcgttgt tgatcccggc
      481 gctgacggcc ggtgaaggca cgcgcatcct tggcaaggac ctaccgctgg aagaaaccat
      541 cgccaatatg tcggggatca tggccaagct cggcatgaag attgagatcg cttcgtggcg
      601 caatatcgtg cccaacgtgt ggtcgctgca tatccgcgat gcgcattcgc cgatgtgttt
      661 taccaacgga aagggcgcaa ccaaggaagg cgcactggcg tcggcgctgg gcgagtttat
      721 cgagcggctg aactgcaatt tcttctacaa cgatcagttc tggggcgaag aaatcgccaa
      781 tgcggcgttt gtgcattacc cggatgagcg ctggttcaaa ccggggccca aggatgcgtt
      841 gccagcggag attctcgacg agtactgcct ggcgatttac aacccggacg acgagctgcg
      901 cggctcacac ctgtatgaca ccaactccgg caacacgctg cgcggcatct gctcgctgcc
      961 gtttgtgcgt cagtccgatg gcgaggtggt gtacttcccc tccaacctga tcgagaacct
     1021 ttatctcagc aacggcatga gcgccggcaa caccctggcc gaggcgcagg tgcagtgcct
     1081 gtcggaaatc ttcgagcgtg cggtcaaacg cgaaatcctc gaaggcgagc tgtgcctgcc
     1141 tgacgtgccg caggaggtgc tggccaagta cccggccatt ctcgctggga ttcagggcct
     1201 ggaagctcag ggtttcccgg tattggtgaa ggacgcatcc ctgggcggcg aattcccggt
     1261 gatgtgcgta accctgatga acccacgcac cggcggcgtg ttcgcctcgt tcggcgccca
     1321 cccaagcttc gaggtggcgc tggagcgcag cctgactgag ttgctgcagg gccgcagttt
     1381 cgagggcctg aatgatctgc cgccgccaac ctttgaaagt caggcgctga tggagccgaa
     1441 caacttcgtc gagcatttca tcgactccag cggcgtggtg tcgtggcgct tctttagcgc
     1501 caaggcggat cacgaattcg ttgagtggga cttctccagt cagggcgagc acgccaacac
     1561 cgaggaagct gcgaccctgt tcggcattct cgaagccatg ggcaaagagg cgtatatggc
     1621 ggtgtacgag cacctgggcg ccacggcctg tcgcatcctg gtgccgggct attcggagat
     1681 ctatccggtc gaggatctga tctgggataa caccaacaag gcgctgctgt tccgcgcaga
     1741 catcctcaac ctgcacagcc tgaatgatcg cggcctcaag tccttgctgc gcaatctgga
     1801 aaacagcgat gtcgacgatt acaccgatat caccacgctg atcggcgtcg agttcgacga
     1861 caacacggtg tggggccaac tgaccattct tgagctgaag ctgctgatcc acctcgcgct
     1921 gaagaaattc gacgacgcca aggagctggt cgagaccttc ctgcagtaca acgacaacac
     1981 cgtcgagcgc ggcctgttct atcaggcgct gaatgccgcg ctggaagtgc agatggacga
     2041 tgagctggag atggccgact tcgagcccaa cttccgccgt atgttcggca aggagcggat
     2101 ggatgcggtg ctcggctcgc tagacggcag cgtgcgcttc cacggcctga caccaaccaa
     2161 catgcagctg gaaggcctcg accggcatct gcgcttgatt gagagctaca aaaagctgca
     2221 cgcggcgcgg gccagagtgg ctgctgtttc gggttgatgc aggatgtagg tttaacctgt
     2281 aggagcgggc ttgcccgcga accgaggtga ctggcaaaaa tcgcggccaa ggccgctcct
     2341 acaggtctgg ctgagcccgc tcgcattgcc acaacataca cgagggaacg tatgagcttt
     2401 gtaataggga acttcaaatg gctgatgctg gtgtccggcg tgctcaccgc cagcatgttc
     2461 tacggcctgt tcgcgccgca ggctgcactt gagtcgatgt ttggggcgtc gtttaccggc
     2521 cagctggcgt ccatcgtgat tcgcagctgg tcagcgctgg ttggcctgat gggcgtgatc
     2581 ctgatttatg gcgcgctgaa cgaaaaacac cgcgccttct gcgcggccat cgcggcaacg
     2641 agcaaggcga ttttcgtcac cctggtgctg gtgtatggcc aggcgttttt gagcaaggct
     2701 gctgcagcga tcatcatgga tatcgtggta atcagcgcca cgcttatctt cctgctggcg
     2761 ttgcgtatca agcgctaggg cgcctagcgt catccaccct acgtgctggc cagcaccgcg
     2821 cagttgcggc cctgatgttt ggctttgtag agcaggcgat ccaccgtttg aagaaaggcc
     2881 tggggctggt cgaacgggcc gggcgtcagg gtgccgacgc cgaggcttaa ggtcagcagt
     2941 gggcttacct gagagtgggc atgctggatg ttctgttcgc ggatcagttg gcggcagcgc
     3001 tcggcaacct gggcggcggc ctgggcatcg gtttccggca gcagcaggac aaactcttca
     3061 ccgccatagc gggccatcag gtcacgcggg cgcacggcag cgccactcag ggcctgacca
     3121 acgcttttca ggcaatcgtc gccctggata tgaccgtagt ggtcgttgta ttccttgaag
     3181 tagtcgatat ccagcaggat cagtgacagc ggcaggccgg tgcgctgggc gttactccat
     3241 tccacctgca ggcggctgtc gaacatgcgc cggttggcca cgccggtgag gccgtcgagg
     3301 taggaaagct cttcgagctg tcgctgcaat tgcagcagct gctgctcggt ctgcttgcgc
     3361 tcgctgatat cgaacatgaa gccgatcagc gactcgacct caccagcttc attacgcagc
     3421 acgtgcacca catcgcgaat ccacacgtaa tcgccatcgc gggtcagggc gcggtagtcg
     3481 gcttcatggt cggtgccggc ctgggactgc gacacgcaga aggccacgac gctgtcacgg
     3541 tcttcgggat gcatgcgctc ggcccaatcg ttagcgctga cccagctggc ctgcggccaa
     3601 ccgagcaggg tttcgatctg cgggccgata taggcgaagg tcatgctcgc ccagtcgatt
     3661 ttccagggga tggccttggt cgactccagc agggtcttgt acaccgcgct gtcggtgctg
     3721 gtcgggtcgg tcatggcagg caatccggac ggctgatagc tgaggtttta accaagatca
     3781 gcgggcggcg caacactggc cgggctgact ggtcagtact gcagcagaat gcacaagggc
     3841 cgctaatgcg gcccttgtgg gttactccga ggtgcgatca ggcatgcacc tgggatgggt
     3901 cacgacggct gtcggggccg ttcatcagcg cggcaccggt ggtttcatcc atcgcctgct
     3961 gcagggcctt cttgcgcttc tcttcggcac ggtgggcgaa gaaccacacc aggaaggtag
     4021 cgaaggacac cgccagcaga atcaggctgg ccacggcgtt gatttccggc ttcacgccca
     4081 gacgcacggc agagaagact tccatcggca aggtcgtgga acccggcccg gacacgaagc
     4141 tggcgagtac cagatcgtcc agcgacaggg cgaaggacat catgccgcca gccgccagtg
     4201 acggcgcgat catcgggatg gtgatcagga agaacacctt ccacggttta gcacccagat
     4261 ccatggccgc ttcttcgatg gacaggtcca gctcacgcag acgcgacgac accaccacag
     4321 ccacgtaggc agaacagaag gtggtgtggg cgatccagat ggtcagcacg ccgcgctcag
     4381 caggccagcc gatcagctgg gccatggcca cgaacagcag caacagcgac agaccggtga
     4441 tcacctcggg cattaccagc ggcgcggtga ccaggccacc gaacagcgtg cgccccttga
     4501 agcgggtcac gcgggtcagc acgaaggcgg ccatggtgcc cagcgctacg gcggcaatcg
     4561 cggtataggt ggcgatttcc agcgagcgca tcaccgagtt catcagctgg gtgttgtcga
     4621 gcaggccgac gtaccacttc agcgaccagc cgccccacac cgttaccagc cgcgaggcgt
     4681 tgaacgagta gatcaccagg atcagcatcg gcaggtagat aaaggtcagg ccggcccaca
     4741 gcataaagtt ggagaaactg aaacgcttca tggtcgaccc tccatctctt tagcttggtt
     4801 acggttgaag agaatgatgg gcacgatcag gatcgccagc attaccaccg ccagagcaga
     4861 ggcaacaggc cagtcgcggt tgttgaagaa ctcctgccac agcaccttgc cgatcatcag
     4921 ggtctccggg ccgccgagca gttcaggaat cacgaactca ccgaccaccg ggataaacac
     4981 cagcatgcag ccggcgatga tgccgttctt ggccagcggc acggtgattt tccagaagct
     5041 gttgtaagtg ctcgaaccca ggtccgcagc ggcttccagc aggctctggt cgtgttttac
     5101 caggttggcg aacagcggca gaatcataaa tggcaggtac gaatagacga taccgatgta
     5161 caccgccagg ttggtgttga ggatctgcaa cggctcgttg atcaccccca gccacatgag
     5221 gaagccattg agcaggccgt tgttgctgag gatgcccatc caggcataca cgcggatcag
     5281 gatcgccgtc caggtcggca tcatgatcag cagcaacagg accatctgct tgtccttgtc
     5341 ggcacgggcg atggcatagg ccatcgggaa gccgataacc agacagagca gggtgctgat
     5401 cgtggccatc ttcagcgagc ccatataggc cgccaggtac aacgcatcct cactgaggaa
     5461 gatgtagtta cccaggttga gcaccagggt gaatttgttt tccacccagg tgtacacgtc
     5521 ggtgtacggc gggatggcca cgtcggcttc ggcgaagctg atcttcagaa cgatgaagaa
     5581 cggcaacata aagaacagga acaaccagag gaacggcacc ccgataacca ggtgccggcc
     5641 cgtgggcatg cggcggctga tgcttcggat aatgttcatg agcgcaatgc caccccgcta
     5701 tcgtcctccc accagacgaa cacttcatcg tcccaggtag gccgggcgcc acgccgttcg
     5761 gcgttggcaa cgaaggactg caccaccttg ccgcttggca gcagcacgtg gaataccgag
     5821 tggccgccga ggtaggcgat gtcgtgcacg gtgccgcgcg accagttgtg ctcgcaggtt
     5881 ggctgctcgg tggtaaccag cagtttttcc gggcggatgg cgtaggtgat gctcttgtcc
     5941 tgtaccgagg tgctgacgcc gtggccgacg tagatattgc gttccagatc cgggctggcg
     6001 atcagcgcgt ggccttccat gtcctcgacc acctggcctt cgaacaggtt gacgttgccg
     6061 ataaattcgc agaccaggcg gctggtcggg gtttcgtaga tgtccaccgg gctgccgatc
     6121 tgggcgatcc agcccaggtg catgatggcg atacgctggg ccatggtcat ggcctcttcc
     6181 tgatcgtgag tcaccatcac gcaggtcacg ccgacgcgct cgatgatctc gacaagctca
     6241 agctgcatct gcgaacgcag cttcttgtcc agagcaccca tgggttcgtc gagcagcagc
     6301 agcttcggcc gcttggccag cgagcgagcc agggccacgc gctgacgctg accaccggag
     6361 agctggtgcg gcttgcgctt ggcgtactgg gtcatgtgca ccagcttgag catttcttca
     6421 acgcgggcgt cgatttcggc tttcggcagt ttgtcctgct tgaggccgaa ggcgatgttg
     6481 tcagccacgc tcatgtgcgg gaacaacgcg taggactgga acatcatgtt gatcggccgc
     6541 tcgtatggcg gcaggtcggt aatgtcgacg ccatcgagaa tgatgcgccc ttcggtcgga
     6601 cgctcgaagc cggcaagcat gcgcagcagc gtcgacttac ccgaaccgga gccaccgagc
     6661 agggcgaaga tttcaccctt gttgatggtc agcgaaacgt catcaacggc gatggtttca
     6721 tcaaacttct tggtcacgcg ctcgatcttg accagcacct ctttcggttg ctgatcaccc
     6781 tcgagaacct ttttataggc actggaagca actgccatta ccaaaactcc cgcaaaaagt
     6841 agcgcccgac cacgctggcc gggcgtttga aattatttac ccgatttgac cttggtccag
     6901 ctgcgcgtca tcaagcgttg tactttcacc ggcaactccg ccgagataaa caacttgtcc
     6961 agcaccgctt gtggcgggta caccgactcg tcgttacgaa cttcctggtc catcagctcg
     7021 cctgccttga tattggggtt ggcgtaaccg acgtaatcgc tgacgccggc aatggccgcc
     7081 ggatccagca gatagttgat aaaggcgtga gcttctttcg ggttgctggc atccaccggg
     7141 atggcgagca tgtcgaacca caggttggcc ccttcttgag ggatgctgta ggcgacttca
     7201 atgcctttac cggcctcttc ggcgcggtcg gcggcctgca ggatgtcgcc agaaaaaccg
     7261 accgcgacgc agatatcgcc gttggccagg tcgccgatgt acttggagct gtggaagtag
     7321 gtcacgtagg gacgcacggc cagcagcttg gcttcggcct tggcgtagtc ctcggcgctc
     7381 tgggtgttgg ggtcgaggcc catatagttg agtacggccg ggatcatctc atcggccgag
     7441 tcgaggaagc tgacgccaca gctggcgagc ttctgcatgt tctcgggttc gaagaccgtt
     7501 gcccaggagt cgatctcgtc gaccccgagt acttccttga tcttggccac gttgtagcca
     7561 ataccgttgg tgccccacag ataaggcacc gaatactggt taccgggatc gttgacttcc
     7621 agttgcttga gcaggaccgg gtcaaggttt tcccagttcg tcagctgact gcggtcgagc
     7681 ttctgaaacg cgcccgcctt gatctgcttg ccgaggaaat ggttggacgg caccaccacg
     7741 tcgtagccgg aacggcccgc cagtagtttg ccttccaggg tttcgttgga gtcgaacacg
     7801 tcgtacaggg ctttgatgcc cgtggccttc tggaaatcgg ccagggtggt ttcaccgaca
     7861 tagtcagacc agttgtagac cttgaccaca ggctcggcgg cttgcgcggc ggagacgctg
     7921 acggcggcgg ccaacagact ggcgagaaga gggatacgac gcataggagc ttccttattg
     7981 ttgtgggtga atgcggcgga gcaacccgcc gcacaccgtt ttggctatgg cctcttgccg
     8041 ttatggcatt agcgacccga tttgatcttg gtccagctgc ggttcatcgc gcgttgtgcg
     8101 ttagctggca gatccgggaa ggtgtagatc ttggccatgg tggccgcgtc cgggtagatg
     8161 cccgggtcgt tacgcagcac ttcgtcaacc agcggggtgg ccgcagcgtt accgttaggg
     8221 aactggacga agttggtgat gttggccatg acgtctggct tcatcaggta gttgaggaac
     8281 acgtgagcgg cttcgacgtt tttcgcatca gccgggatag ccatcatgtc gaagaagctg
     8341 ccagcacctt ctttcgggat gctgtaggag atgttcacgc cgttcttggc ttcttcagca
     8401 cgggacttgg actggtacag gtcgcccgag tagccgatgg ccacgcagat gttgccgttg
     8461 gccaggtcgc cgatgtactt ggagctgtgg aagtaagagg tcgaggaacg gatcgagagg
     8521 aacagctctt cagctttctt cagctcgcct gtatcagtgc tatccggctt gaagcccaga
     8581 tagttcagcg cagccgggag aatttcggtt ggcgagtcga ggaaggacac gccgcactct
     8641 ttcagcttgg cgatgttttc cggcttgaac accaggtccc aggagttgac cggtgcgtct
     8701 tcgcccagtg cggctttgac cttgtcgacg ttgtaaccga tgccgatggt gccccacatg
     8761 taaggaatcg agtactggtt acccgggtcg cttggatcga gggccttgag caggctcttg
     8821 tcgaggtttt cccagttgga cagcttggac ttgtccaact tctggaatac accggcctta
     8881 atctgcttgg ccaggaacgg gttggacggc acgacgatgt cataaccgga gctgccggcc
     8941 agcagtttgg cttccagggt ttcgttgctg tcaaagacgt cgtagacgac cttgatgccg
     9001 gtttccttct ggaagttgtc gagggtgtct tctgcgatgt agtcggacca gttgtaaacg
     9061 tgcaggacct tgtcatccgc gtgggcggca cttactacgg ccccagcaag ggacaacgcg
     9121 agaagggttt tgccgaatgt gttcatgcgt acagctcctg ttgttgtagt cgttttccgg
     9181 aagatgcgca gccatctgca gcaccttccg gtgagcctga cctacgcatt ggcg
//
LOCUS       DASRUM010000086         3089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_209785, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000086 DASRUM010000000
VERSION     DASRUM010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209785"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..1124
                     /locus_tag="VFF22_05830"
     CDS             303..1124
                     /locus_tag="VFF22_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter"
                     /protein_id="HZX16368.1"
                     /translation="MDLPNEWIALLALVFILGAKHGLDADHLATIDGLTRFNLRFAPH
                     RARWCGSLFSLGHGTIVMLIALGVGLMSTQWQVPEWMEDLGTWISIGFLTLLGILNLR
                     AVLTAPAGAMVATIGLKAGLLRRLQATSHPLAIAAVGALFALSFDTMSQAALFAVTAT
                     QHGGLVHTLTLGLAFTIGMLLADGANGLWIARLLRRADHRARIASRIMGLMVAAISFA
                     VAGFGLARWLHADLSQWYEGKELLVGFGVIALLAGSFVLGDRLARSSRLAGATTD"
     gene            1213..2763
                     /locus_tag="VFF22_05835"
     CDS             1213..2763
                     /locus_tag="VFF22_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZX16369.1"
                     /translation="MGESKLKRAKHEEMLLSCAGVETAGGRVQVRWESDSAATPMGQL
                     AYFIEFLTLTGLWSRWQESCPLAYVSPNAPTKAEVLGTWMLSVLSGHKRYSHVTTIRC
                     DGVNPGLLGMRRVISEDALRRALTAIPEEDGVAWLDGHLNESMAPLLDAPWILDIDTT
                     VKPLYGKQEGAVVSYNPKKPGRPSHSYHTYLMAGLRLVVGAEVKAGNEHSGSHTLPGL
                     LKILDALPVNRKPKMVRGDCGFGTDGIMAALEARQQPYLFKLKLSKNVKRHIERIFWD
                     AGWCDAGQGWEGKDGELRLSGWDAARRVIVLRRPLQGEMLITEDDNGQGLLGFIEADR
                     KTGKRTTGYEYAVLVTNLDHEIVSLGQLYRDRADAENAFDELKNQWGWGGFTTHDLHR
                     CQLSARAVALIYNWWSLFVRLASPEARREAITSRPWLMSSVGRKTEHAGQTTITLTGL
                     HAHFGKAREALMRVSALLKGWVTRTAEQFNSTTVWTLCCDHIKQTIARIGPSQTHRFL
                     AHHANGIG"
     gene            complement(2687..>3089)
                     /locus_tag="VFF22_05840"
     CDS             complement(2687..>3089)
                     /locus_tag="VFF22_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004882376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZX16370.1"
                     /translation="CMTTGVILVHEKIAARVTEQLVAKASHLPVGDPATQQVALGPLI
                     SERQRDRVHGIVQDSIEQGAQLAAGGSYEGNFYKPTVLTQVKPGMRCFDEEVFGPVAS
                     IILKTAVTRFHSHDVREICVFETARFGRWSA"
BASE COUNT          540 a          979 c          983 g          587 t
ORIGIN      
        1 ggccgatgcc gcaaccgcgc tggccggtgc cgacccggcg cgggatctgc acgccgccga
       61 ggtgatggga ccgtcgcgct cgtccgaagg cgtgcggctc tactacctgg tgcgctggct
      121 ggactgattg tcctggatag cgcggtcccc aaagccgcat agccgctaac caccgtcaat
      181 gcgagtcttc aagcttaact cgctgatccg aatgcgttta caccgctgcc attgtccggc
      241 atggattttg tatgaggaat tcaatagact tcataccatc cacgctgacg aggcccgccc
      301 cgatggatct gcccaacgaa tggattgccc tgctcgccct ggtgttcatc ctcggcgcca
      361 agcacggcct cgacgccgac cacctcgcca ccatcgacgg cctgacgcgc ttcaacctgc
      421 gtttcgcgcc gcaccgcgcc aggtggtgcg gctccctgtt ctccctcggt cacggcacga
      481 tcgtcatgct gatcgcactg ggcgtcggcc tgatgtcgac gcagtggcag gtaccggaat
      541 ggatggaaga cctcggcacc tggatttcga tcggtttcct gacgctgctc ggcatcctca
      601 atttgcgtgc cgtattgacg gcgccagccg gcgccatggt cgccaccatc ggcctcaagg
      661 ccggcctgct gcgtcgcctg caggccacct cgcatccgct ggcgattgcc gccgtcggtg
      721 cgctgtttgc gctgtccttc gacaccatga gtcaggccgc gctgttcgcc gtcaccgcca
      781 cccagcacgg cggactcgtt cacacgctga cgctcgggct ggccttcacc atcggcatgc
      841 tgctcgccga cggcgccaac ggcctgtgga tcgcccgcct gctgcgtcgc gccgaccatc
      901 gcgcacgcat cgcctcacgg atcatgggcc tgatggtcgc cgccatcagc ttcgccgtgg
      961 ccggcttcgg cctggcgcgc tggctgcacg ccgacctctc gcagtggtac gagggcaagg
     1021 aactgctggt aggtttcggc gtgatcgcgc tgctggccgg ctctttcgtt ctcggcgaca
     1081 ggctggcgcg cagctcgcgc ctggccggcg ccacaaccga ctgatttatc ctaaaaaacg
     1141 catttgactg cgtgcaaagt agccacaggt caggcaggct gtgggcttga tcggtctgcg
     1201 gggagacacc tgatgggtga gtcgaaattg aagcgggcga aacacgaaga aatgcttttg
     1261 tcgtgtgctg gggtggagac ggcgggagga cgggtacagg tacgctggga gtccgacagc
     1321 gcagcgacgc cgatgggtca gctggcctac tttatcgagt ttctgaccct gaccggtctg
     1381 tggtcgcgct ggcaggagag ttgcccgttg gcctacgtga gccccaatgc accgaccaag
     1441 gcggaggtgc tgggcacctg gatgctgtcg gtcctgtcgg gacacaaacg gtattcgcac
     1501 gtcaccacca ttcgttgcga tggcgtcaat ccgggacttc tgggcatgcg ccgggtcatc
     1561 tccgaggacg ccttgcgccg agcattgacg gcgatcccgg aagaggacgg agtggcctgg
     1621 ctggacggtc acttgaacga gagcatggcg cccctgctgg atgccccgtg gattctcgac
     1681 atcgacacca ccgtcaagcc gctctatggc aaacaggaag gcgccgtcgt gtcctacaac
     1741 ccgaagaagc cggggcgacc gtcgcacagt taccacacct acctgatggc cggcctgcgc
     1801 ttggtcgtgg gcgcggaggt caaggccggc aacgaacact cgggcagtca caccctgccc
     1861 ggattgctga agattctcga tgcgctgcca gtgaaccgga agccgaagat ggttcgtggt
     1921 gattgcggat ttggcacgga cggcatcatg gcggcactgg aggcacggca gcagccgtac
     1981 ctcttcaagc tcaagctttc gaagaacgtc aaacgtcaca tcgagcgcat cttctgggat
     2041 gccggatggt gtgacgccgg ccagggttgg gaaggcaagg acggcgagtt gcgcctctcg
     2101 ggttgggacg ccgcccgacg ggtcatcgtt ctgcgtcggc ccttgcaagg cgagatgttg
     2161 atcaccgagg acgacaacgg tcagggcttg ctcggtttca tcgaagcgga ccgcaaaacg
     2221 ggcaaacgta ccactggcta cgagtatgcg gttctggtca ccaacctcga tcacgaaatc
     2281 gtctctctcg gacaactgta tcgagaccgg gccgacgccg agaatgcctt cgacgagttg
     2341 aagaaccagt ggggctgggg tggctttacc acccacgacc tgcaccgctg ccaactgtcg
     2401 gcccgcgccg tagcgctgat ctacaactgg tggagtctgt tcgtccggct ggcaagtcca
     2461 gaggcgcggc gagaggcgat taccagccga ccgtggttga tgtcctcggt cggtcgcaaa
     2521 acggagcatg ccgggcaaac caccatcacc ctcaccggct tgcacgcaca cttcggcaaa
     2581 gccagggagg ccctgatgcg tgtctccgcc ttgctcaagg gatgggtgac ccgcactgcg
     2641 gagcagttca actcgacgac cgtctggact ttgtgctgcg accatatcaa gcagaccatc
     2701 gcccgaatcg ggccgtctca aacacacaga tttctcgcac atcatgcgaa tggaatcggg
     2761 taactgccgt ttttaggatg atcgaggcca ccgggccgaa cacttcctcg tcgaagcagc
     2821 gcatgccggg cttcacctgg gtgagcaccg tcggcttgta gaagttgccc tcgtagctgc
     2881 cgccggcggc gagttgcgca ccctgctcga tgctgtcctg gacgatgcca tggacgcggt
     2941 cgcgctgccg ttcgctgatc aggggtccga gcgcgacctg ttgcgtcgcc ggatcgccga
     3001 ccggcaggtg gctggccttg gcgaccagtt gctcggtcac ccgggcggcg attttttcgt
     3061 gcaccaggat gacgccggtg gtcatgcag
//
LOCUS       DASRUM010000087         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_705771, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000087 DASRUM010000000
VERSION     DASRUM010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705771"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..2295
                     /gene="mutS"
                     /locus_tag="VFF22_05845"
                     /pseudo
     CDS             5..2295
                     /gene="mutS"
                     /locus_tag="VFF22_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790431.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
BASE COUNT          466 a          763 c          683 g          383 t
ORIGIN      
        1 aacgatgagc caaagcgacc tcagttctca cacgccgatg atgcagcagt actggaagct
       61 gaaaaatcag cacccggaac agctgatgtt ttaccgcatg ggcgacttct acgagatctt
      121 ctacgaggac gcgaaaaagg ccgccaagct gctggatatc accctcaccg cccgcggcca
      181 atccgccggc caagccatcc ccatgtgcgg gattccctat cactcgctgg agggctacct
      241 ggtcaagctg gtcaagctcg gcgagtcggt ggtgatctgc gagcagatcg gcgacccggc
      301 caccagcaag ggcccagtgg aacgccaggt ggtgcgtatc atcacccccg gcacggtcag
      361 cgacgaagcg ctgctcgacg agcgccgcga caacctgctg gccgcggtac tgggcgatga
      421 gcgcctgttt ggcctggccg tgctggatat caccagcggc cgcttcagcg tattggagat
      481 caagggctgg gagaatttgc tggcagagct ggaacgcctg aacccggttg aactactgat
      541 tcccgatgat tggccgcgcg acctgcccgc cgaaaagcgc cggggcgcaa aacgccgcgc
      601 gccttgggac ttcgagcgcg acacagctca taagagcctg tgccaacagt tcgccaccca
      661 ggacctgaaa ggcttcggct gcgaaaacct gactctggcc atcggcgctg ccggctgcct
      721 gctggcttac gccaaggaaa cccagcgtac tgccctgccg cacttgcgca gcctgcgcca
      781 cgaacgcctg gacgacacgg tgattctcga cggtgccagc cggcgcaatc tggagctgga
      841 tatcaacctc gcaggcgggc gcgagaacac cctgcaatcg gtagtcgacc gctgccagac
      901 cgccatgggc agccgcctgc tcagccgctg gctaaaccgt cctctgcgtg accaggccat
      961 tctggaagca cgccaggagt cgattgcctg cctgttggag cgctaccgct tcgaaaccct
     1021 gcagccgcag ctgaaagaaa tcggcgacct ggaacgcatc ctcgcccgga ttggcctgcg
     1081 taatgcacgc ccccgtgact tggcgcgcct gcgtgacgcc ctcgccgccc tgcccacact
     1141 gcagatggcc atgaccaatc tggaagcgcc gcacctgagc gaactggcag gcgtagtcag
     1201 cacctacccg gagctggccg agctactggc ccgggcgatt atcgacaacc cgccggcggt
     1261 gatccgcgat ggcggtgtgc tcaagaccgg ttacgacgcc gagctggacg agttgcaatc
     1321 gctcagcgag aacgccggcc agtacctgat ggacctggaa acccgcgaga aggcccgcac
     1381 cggcctggcc aacctcaagg tcggctacaa ccgtgtgcac ggctacttta tcgagctgcc
     1441 gagcaagcag gccgagtcgg ctccggccga ctatatccgc cgccagaccc tcaaaggcgc
     1501 agagcgcttt atcacgcctg agctgaaaga gttcgaagac aaggcgctgt ctgccaaaag
     1561 ccgcgccctg gcccgcgaga agatgctcta tgacgagctg ctcgaacgcc tgatcagcca
     1621 cctcggcccg ctgcaggaca gcgccgcagc cctggcctaa ctggacgtat tgagcaacct
     1681 ggcggagcgc gcgctgaatc tggatctcaa tcgcccacgc tttgtcgatg agccctgcat
     1741 gcgcatcgac cagggtcgcc acccggtggt cgaacaggtg ctgaccacgc cgtttgtggc
     1801 caacgaccta agcctggacg acgacacgcg catgctgatc atcaccgggc cgaacatggg
     1861 cggtaaatcc acctatatgc gccagaccgc actgatcgtg ctgctcgcgc atatcggcag
     1921 cttcgttccg gcgaaaagct gtgaactgtc gctggtggat cgcatcttta cccgcatcgg
     1981 ctcaagcgat gacctggccg gtggccgctc gacctttatg gtggagatga gtgagaccgc
     2041 taatatcctg cataacgcca gtgaacgcag cctggtgctg atggatgaag tgggccgcgg
     2101 taccagcacc ttcgacggtc tgtcgctggc ctgggccgcc gccgaacagc tggccaagct
     2161 gcgcgcctac accctgttcg ccacccatta tttcgagctg accgtgctgc cggaaagcca
     2221 gccaatcgcg gccaacgtgc atttgaatgc caccgagcac aatgaacgca tcgtgttcct
     2281 ccaccacgtg ctgcc
//
LOCUS       DASRUM010000088        34184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_48873, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000088 DASRUM010000000
VERSION     DASRUM010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 34184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34184
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48873"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFF22_05850"
     CDS             <1..203
                     /locus_tag="VFF22_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011105258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZX16371.1"
                     /translation="HGKGILIKKALASGHACLAPGVDPVRASFELIFGHPGACSAIIG
                     TINPQHLAHNVATAAAVIQDIA"
     gene            267..1187
                     /locus_tag="VFF22_05855"
     CDS             267..1187
                     /locus_tag="VFF22_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003236303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATPase"
                     /protein_id="HZX16372.1"
                     /translation="MPRTLIRKDPSNFKTLQLFVDASPEGLSYQGLGMPLNFAQMLEK
                     RKPVNVADTQRFAVELANLGVSVRLTLCWQGREYWVLVRQRRADRGDVVLKLISGYVP
                     AHELNLPLLTAIQEVAEECLLETPEGWLSGRFGDTWLPTPYQSSLRYRETAHFSLSSL
                     SGAARPVQCGNLKLLERPRAYVHLPTASLQLVYDLRLELPKDTRQLSLLHVDEHLEDG
                     QLVARLNRARPDLFLIPLDQGRPTAELLTLKQGQLSPASTRGLWLAESFAPQGGWLVR
                     DERIRWKDWLVQQNCADPAAKNSRAQKSPA"
     gene            complement(1217..2425)
                     /locus_tag="VFF22_05860"
     CDS             complement(1217..2425)
                     /locus_tag="VFF22_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZX16373.1"
                     /translation="MNLETPKKFRGLVNQAHQVAENYFRPISRKYDKAEHAYPKELDL
                     LAALLDGMNAGSPDAIGATSASKRGATREQQEGVKNGGNLSALLGVMELCWGDVGLLL
                     AMPRQGLGNAAIAAVANDEQLARFGGTWAAMAITEPGCGSDSAAIRTTAIKDGDHYVL
                     NGEKIFVTSGERADSVVVWATLDKNLGRSAIKSFVVEHGTPGMSVTRLEKKLGIKASD
                     TASISFSDCRVPAANLLGNPEIDVQKGFAGVMETFDNTRPLVAGMAVGVAKASLDRAR
                     ELLKKVCKLDYRTPLLSVSHAEATLYRLEAEWEAARLLTMKAAWMADNKLPNSKEASI
                     AKAKAGRVANEVTLKCVELAGAMGYGEDELLEKWARDSKILDIFEGTQQIQLLIVARR
                     LLGKSSSELK"
     gene            complement(2437..3720)
                     /locus_tag="VFF22_05865"
     CDS             complement(2437..3720)
                     /locus_tag="VFF22_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZX16374.1"
                     /translation="MTSDVQGRALAVLNRVAQADWPDRFKLRKPFEKLIYTGSRASFR
                     AAAQRAGKVARLPRPVDPDALFDLSLSDEQQMLVEMLEAFAGEVLRPLAHDADEQAAL
                     PAALLNQAHELGLTHYAVSEAQGGMAGERTTLSNALIAEALGKGDLSLAAALLVPLSA
                     ANCIRRWGSAAQQARWLPAFVGEEPAAVMAIAVNEPTPLFDPHKLATRARKRGKHYVL
                     SGEKCLVLRGLDASQLIIAAQAADGPALFIVEAGAKGLKRSPQPAMGLKAGSTARVRL
                     NGVKVPAEARLAAECFSYQSFLDYAGLAWCALAVGTAQAALDYVVPYCNERVAFGEPI
                     SHRQGVAFMVADMAVELDAMRLMVWRACSRAEQGLSFQREAYLARLLCSEKAMKIGTD
                     AVQLLGGHGFTKEHPAERWYRDLRALAIMAGGLQL"
     gene            complement(3822..5753)
                     /locus_tag="VFF22_05870"
     CDS             complement(3822..5753)
                     /locus_tag="VFF22_05870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HZX16375.1"
                     /translation="MEGAVAQEFRQDINGLRAWAVMAVVLYHFGVTGISGGFAGVDVF
                     FVISGYLMCGIISGGIARGEFSVWRFYLARARRIFPALIVLCVVALIFGWFFLMPEEY
                     KLLGKHARESLTFSSNLRYFEESGYFDVASQEKWLLHTWSLSVEWQFYLILPLVLMLV
                     SKFLPGWRAITIVLGLLFVVSLALCLWRTSVAPSEAFYMLQTRAWEMLAGALVFMFGH
                     RGLPDGLRRALELAGFALILATIVLLDKQSVWPGWRAILPVVGAALVLLAARGHSMWT
                     ANFPAQWLGTRSYSIYLWHWPLVVALAYLELLSDPLWIASAIAVSLFLGHLSYVLVEI
                     PARQRLSLLSAGRAGIAVVVVLALVAIVAQQVRRSGFPDRLPEAVAIVEAERKNHNPR
                     LEDCLDPAASCIYGQEPVRAILIGDSHADAVATALQAALPNEEGGVLFRGGSGCLIAF
                     GMQTTSSDKHYCEKLNADLEKEHASFPAGVPIVMMGRTSKYVQGGLSTTTKPSFHFGE
                     PVEQFSAEFLEQFRERYVTTMCSLAEHRPVYIVRPTPEMGVDVPTLIGRAMLLGRERR
                     FSLSLADYHKRHAFIWAVQDETAKRCGVHLLNPLPYLCSDDKCPSAEGNRPLYRDTDH
                     FTEFGNRLLVPMFRTVFLR"
     gene            complement(5759..6073)
                     /locus_tag="VFF22_05875"
     CDS             complement(5759..6073)
                     /locus_tag="VFF22_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16376.1"
                     /translation="MHTHGQHVRIGADNTVEGGIFRYEAASIDIGNDCVISTDILIGT
                     GDKHSFIDVASNQPESIVRPCSLVTKAFEEGRRVFAGVASSYNEKNMVPVGALYTVEF
                     GW"
     gene            complement(6073..6237)
                     /locus_tag="VFF22_05880"
     CDS             complement(6073..6237)
                     /locus_tag="VFF22_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16377.1"
                     /translation="MLDHLDLSLRNLACRNCLVFGEGVEVSHCEIRLDGEGNLIEIRT
                     RLRPASAKST"
     gene            complement(6241..7665)
                     /gene="hldE"
                     /locus_tag="VFF22_05885"
     CDS             complement(6241..7665)
                     /gene="hldE"
                     /locus_tag="VFF22_05885"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HZX16378.1"
                     /translation="MKLSMPRFDQAAVLVVGDVMLDRYWHGGTSRISPEAPVPVVKVE
                     QIEDRPGGAANVALNIAALGAPATLLGVTGQDEAAESLRNSLQAVGVKTFFQTIKDQP
                     TIVKLRVMSRHQQLLRMDFEEPFRTDSAALAASVEAQLDGIKVLILSDYGKGALQNHQ
                     TLVQLALSKGIAVLADPKGKDFSIYRGASLITPNLHEFETIVGPCRDEAELVAKGAAL
                     MSELELGALLVTRGEHGMTLLRPDQPALHLPARAREVFDVTGAGDTVISTLAASLAAG
                     EELPHAVALANLAAGIVVGKLGTAAISAPELRRAIQREAGSERGVLSVDQLLLAIEDA
                     RAHGEKIVFTNGCFDILHAGHVTYLEQARAEGDRLVLAVNDDASVSRLKGPGRPINAV
                     DRRMAVLAGLGAVDWVVSFSEDTPENLLRAVKPDVLVKGGDYGIEQVVGADIVTAYGG
                     EVRVLGLVENSSTTAIVEKIRSQD"
     gene            complement(7731..9131)
                     /locus_tag="VFF22_05890"
     CDS             complement(7731..9131)
                     /locus_tag="VFF22_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008074650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZX16379.1"
                     /translation="MSARKQTLLKQHRRQKRIALLVALVLLCLLGIFAAWWWVPLLLL
                     VGWIAHEAWFADHLFYAPDDDYSYAFAQDVPALAGRIEAGRLLLDGDIGAADTLILQV
                     QLKASWLGRWLDPYVLIGTDRQDFERGVNGCRYLNLSGQQDALATGTLQLRGRFCRIE
                     PAVTLFALSNPDYAQQRVMVIAPHADDAELAAFGLYSRARDVSIVTLTQGEIEAEYYQ
                     VLGLDQAAAARLKGRLRAWDSLAVPLWGGVPQSQCLQLGYYCLQLPAMQAQPEAAFAS
                     RESGEGDTRSVRAANSIQLPGDADGLPSWCNLLADLVAALEHFQPEVVVTPHPQLDPH
                     SDHVASTEALLQAVELSSWKPKTLLLYANHLHDNDRWPMGPAGFGIALPPAIEALPAD
                     GLWSPGLDAARQLDKAMALAMQHDLQGALPLKRRIRRLIQRLLVGRRWPATGEDEFFR
                     KAVRRHELFWVRTLDG"
     gene            complement(9134..10030)
                     /locus_tag="VFF22_05895"
     CDS             complement(9134..10030)
                     /locus_tag="VFF22_05895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013984211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX16380.1"
                     /translation="MLSHLRVWRERGWTLIDAAAYADAWQRFGGSVATHPQVIERLVG
                     LAGLPVRYLGWFERGELRAAVPTWGRHLALSKDVLKQQGKRGLFDLGNAEVILPLASD
                     ARVPLRHKVRYLSALNAEQVIGRREQPEGLALAREPEEYSKKFRYNQRREQRLLEEAG
                     GVVRPMLELSAAEQAVIYADLFQRRWGFEATGQANLAEVFSLMRDFMTGSLIYLNDQP
                     VAIQVLYRVESPQWVSLEYINGGVDPQNREFSPGSVLSFINTQTAWAEARAIGKPLRY
                     SFGRADREYKDRWCNRMPVYQV"
     gene            complement(10030..11856)
                     /gene="msbA"
                     /locus_tag="VFF22_05900"
     CDS             complement(10030..11856)
                     /gene="msbA"
                     /locus_tag="VFF22_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HZX16381.1"
                     /translation="MANSTQPSSVKVYLRLLRYVIPYWGLFAVSILGFMLFAVTQPML
                     GYMLKFFVDGLNNPNANFFAAVPYLKDMPWLGELKLLQAVPLLIVLIALLQGISSFLG
                     NYYLAKVSLGLVHDMRVLLFNNLLTLPNRYFDNHNSGHLISRITYNVTMVTGAATDAI
                     KVVIREGMTVVFLFATLLLMNWKLTLVMVAILPIIGLMVVSASKKFRKQSKKIQVAMG
                     DVTHVASETIHGYRVVRSFGGEGYEETRFAQASTENRLKQLKMVKTNAVYTPSLQLVI
                     YSAMAGLMFLVLLLRGDATAGELVAYITLAGLLPKPIRQLSEVSSTIQKGVAGAESIF
                     EQLDEAPEIDLGTQERERVSGRLDVRNLSFQYKGSEKPVLDDISFVAEPGQMVALVGR
                     SGSGKSTLANLIPRFYHHDQGQILLDDIDVEDYKLRNLRRHIALVTQHVTLFNDTVAN
                     NIAYGDLAGAPLEDVKRASESAYAAEFIDQMPQGYDTVVGENGVLLSGGQRQRLAIAR
                     ALLKDAPLLILDEATSALDTESERHIQAALDEVMKGRTTLVIAHRLSTIEKADLILVM
                     DKGRIVERGNHAQLLAQNGYYARLHEKDFVEAVESSMPEAEA"
     gene            11892..12491
                     /locus_tag="VFF22_05905"
     CDS             11892..12491
                     /locus_tag="VFF22_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HZX16382.1"
                     /translation="MRIVTAQELESWLASGTVLEKDSRGPKVLMLSDGQILKIFHTRS
                     HPVFSRLNPPAKKFAQNALTLQQLGIPAPQIFESLWIDIAAGISASIYQPLPGQSVES
                     ILKETPEQIDALIPQLAKFIKTLHRQGVYFRSLHLGNILQLQNGQFGLIDFLDLTKKS
                     RSLSSWEVNRNFKHLERYLMRRKLMSFPYKKLMNTYSKL"
     gene            complement(12501..13349)
                     /locus_tag="VFF22_05910"
     CDS             complement(12501..13349)
                     /locus_tag="VFF22_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZX16383.1"
                     /translation="MDHEAYERVLSAYDAALSFRRGALESDFVGSGKGRYTLNSYRKI
                     VLQDSVLFEKIYLRDSVDWRKCNFFYKHVLSALSGDELSVPRLLDVVSGERLVVSRFE
                     FVDFRAIDESDYIDRLVSVTRRLASFEYDSTLVLGEFKSLELHFGFERCLRKTQAVLE
                     QIGEKNSVLLKMKERCEAMPRFVGHGDLSLPNMGVGSIVLDWDNFGFYPPGFDLALGS
                     VLKGELLNAEALNSMVERVFPSFSTRCTFDMLWFSFVFFYCVFLSARQAEHKHYVYTL
                     LVKRFS"
     gene            complement(13478..14323)
                     /locus_tag="VFF22_05915"
     CDS             complement(13478..14323)
                     /locus_tag="VFF22_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core biosynthesis protein"
                     /protein_id="HZX16384.1"
                     /translation="MSCSDVVDSSLLQLDSGQCIALHDFAENRGRFSGAVFLVASGPS
                     VLTFPMQRYSHIPMIAMNGSIARFIEEGIKPLFYLCDDRGVAKRKGAAVAQGITLAEN
                     SGLGLAALQEVAKHSPHALSTGKLHLLERVNRQRGSAAISDRRFAWSVRNDPEFSVEW
                     SLFRQKRNRIGFSRNLCNGYFNGRTIAYAALQLAYHLGFDRVYLVGVDMSPEQGQFYD
                     PQGLQVPSRLGDDYADYILPSFELMSRRVLSDSFQVFNLSVDSRLPSNVIKKIKYNQL
                     DDLLA"
     gene            complement(14323..15504)
                     /locus_tag="VFF22_05920"
     CDS             complement(14323..15504)
                     /locus_tag="VFF22_05920"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZX16385.1"
                     /translation="MVFQLVRPYTPMLALLSLAIIFLGRFWWPGEASAWAGSARFALL
                     SFLIAYLLSGEIGKGFKAKALLVTAGLLVSWVIVNFLLVGGDSGVLRRSIVLMVFVCA
                     VVVLKDARTDWLRQLLMVVVVVAAVAAFVTLIYQLRARGLDFKYRAFRLSNSGVTGFA
                     QFGNPIISGLYMAFAGLTALWCVLRTNKVWAKSLWFFCLLVIGLYVFLTFSRTAWVAM
                     GLGGAVLFVFQAKRDYFLLAIGVTFALFVSLVLWRFPEILSVELERGATGRHLIWNMV
                     LSSMSGYWWLGHGAGSSMQPMSIPGQQVVNTHSLYLEVLFQYGIVGLALFISSLLLAL
                     RQLWLERSEMAVLGLCLLVGCIGVMFFDLYSFIHSPNLVWLWVWFPLGLALACSKDGV
                     R"
     gene            complement(15558..16214)
                     /locus_tag="VFF22_05925"
     CDS             complement(15558..16214)
                     /locus_tag="VFF22_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HZX16386.1"
                     /translation="MRLLTEYCSLVFDCDGVVLNSNHVKTEAFYQAALPYGEAAAQAL
                     VDYHVANGGISRYRKFEYLLGQILGLPKNSEALDALLAHYAALVHDGLRSCAIAPGLH
                     ELRAQLPDANWLIVSGGDQEELREVFAVRGLAALFDGGIYGSPDAKDMILARELERGN
                     IRMPALFLGDSRFDHQCATAAGIDFVFLSGWSEFKGWPEYFSANPVAVRESLGTLMAE
                     "
     gene            complement(16214..16954)
                     /locus_tag="VFF22_05930"
     CDS             complement(16214..16954)
                     /locus_tag="VFF22_05930"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HZX16387.1"
                     /translation="MSAPDYAVIIPARYQSSRLPGKPLVDLCGVPMVVRTYRQCIQAC
                     PPERVYVATDDERIAAVCRAHEIQVLMTSPDCLTGTDRVAECARQLDTEVLINVQGDE
                     PVFNPQDLRAILESLSQYPGEVLNGFCAIDNDESFRSESIPKVVMRPDGRLLYMSRSP
                     IPGNKQHAFVRAWRQVCVYAFPRAALVAFAEQPRKTPLEALEDIEILRFLELGWEVRM
                     LEMSADSIAVDNPDDVARAEAAICERGL"
     gene            complement(16966..18579)
                     /locus_tag="VFF22_05935"
     CDS             complement(16966..18579)
                     /locus_tag="VFF22_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HZX16388.1"
                     /translation="MSNQALVMLDCTLRDGGYYNNWDFDAELVQDYLRAMAAVAVDYV
                     ELGLRSFESNGFRGAYAYTTDSFLRRLELPEGPGIGVMINASELIKHPAGVLPALELL
                     FAPAADSPVDLVRIACHVHEFEAALPACDWLKAQGYRVGINLMQIADRQPEEIEHLAR
                     QAEGHALDVLYFADSMGSLSPDQTSAIIQALRRGWHGALGIHTHDNRSMALANTLRAV
                     EDGVLWLDSTVTGMGRGPGNVRTEYLVVELMAKRARSVQVAPLLNVIARHFQPLQQRH
                     SWGTNPYYFLAGTYGIHPTYIQEMLTDSRYAEEDLLVVIDFLKHQGGKKYSPGTLESA
                     RHFYSGEPRGNWAPQTLIAGREVLLLGSGPGVARHRQGLEDYIRRAKPFVIALNTQDH
                     LAAELIDVRAACHPFRLMADCQEHRKLPQPLVTPAHMLPADVREALADKTLLDFGIAV
                     QAGQFAFAEQHCVLPTSLVAAYALAIAASGQATRVLLAGFDGYAADDPRGIEVNQLFD
                     AFQQLPGVPDLLAVTPSRYQVPRTSIYVL"
     gene            complement(18576..19841)
                     /locus_tag="VFF22_05940"
     CDS             complement(18576..19841)
                     /locus_tag="VFF22_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007251986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HZX16389.1"
                     /translation="MSQEVMRKARELDRYRWCLLRERHGVLGSALRFAREMLSDLLFG
                     VRARLSLAQVVEGETCDFLLLQSAPKVIAFQRKKLLMQALRERGHVLIETALPEASII
                     VRQRLLCAPLYKVPLRYFGYAAHVQWLMARYQPRILLNDRNGSLYAPFLRMALNQRDA
                     LLVHLAHATTVEGSSRLGMNDYDYYFLFGQSSLEALQARALRFGASTAVLAGSHMIDG
                     AFDLPASDPDRRVMLVLGVGPDKEKEHGYQRTYALLQAWALQNPSYRLLIKAHPRSAV
                     PFWHEAAARSTQVEVLPRDCGLADALKQASVVINIMSNAVIEAALARRPVVYVNAGEQ
                     QDIFSQEQFFGACITDVEQLQQRVLEIEAGYADCVGAAERFADYHLAQGSQGLLRNLQ
                     LLEQLQRTGHCEGVCLPPAHLTEPSRTME"
     gene            complement(19838..20725)
                     /locus_tag="VFF22_05945"
     CDS             complement(19838..20725)
                     /locus_tag="VFF22_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017526463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6625 family protein"
                     /protein_id="HZX16390.1"
                     /translation="MNKQPSIIFLIPYFGQWPFWMPFFVESCRGNPSIDWLLVGDCAP
                     LDNLPPNVKQRHISFADYCAWISERLGFAFAPKSPYKLCDLKPALGYLHEQDVAGYDY
                     WGFSDLDLIYGNLRAYFRDERLRRYKFFSTHERRVSGHLCLLRNEQPLRELFREIPDF
                     QRRVQDQKNHALDEGAFSRLFLWRKNFPKPLFKLVGKFNRWRRVARFKEAFSTPNGVR
                     AWTDGTQDYPEYWTWAPGSLTNAKDGAREFPYLHFLVWKKQWHKQDEPPLTLEQAQAL
                     ASSQSWHVDRKGFHPGATP"
     gene            complement(20725..21855)
                     /locus_tag="VFF22_05950"
     CDS             complement(20725..21855)
                     /locus_tag="VFF22_05950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014597888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZX16391.1"
                     /translation="MTMSEVAGAGQPWVLQFCHGYDGPFLDCARQYAALFVGTPYKVC
                     TVYLTGKPSAEVEQGSASDEVIFLDYSSRQVRGLKLKAIRDLKRIAASRDFKFCIAHR
                     FKPIYVALLGSDLPVIGVHHAFGDYKRRTRQLFANLFRKRLALLGVSNAVRDDMRACL
                     PNWPAERIETLYNRIDIVAVQAEQVSREAAREHLGLPQNAWVVGNVGRLHPDKDQATL
                     IKGFAQALPQLPAGSLLAIMGSGGLEPALKALAQELNVDEQVRFLGQVAYGRRYFKAF
                     DVFALTSDHEPFGMVLLEAMAAGVPVICSDCGGGREVAQGVAQLFPLADAGALAAGLV
                     SLSKLDTATGAALRQGQLLKLGKEYVDEVARPRFWRFLEFSQ"
     gene            complement(21842..23596)
                     /locus_tag="VFF22_05955"
     CDS             complement(21842..23596)
                     /locus_tag="VFF22_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HZX16392.1"
                     /translation="MALTILGLSGALSHDPSAALYIDGKLIAAVEEERFVRDKHAKNR
                     MPYESAKFCLEQAGIKPSDVDVVAIPFAPISIFEKARWQYAKRYWYAPDRALDAILMG
                     NRRYKRYYKHIQWCLQQLGFDLKKIKIEPVEHHLAHAASAYHCSGFTEKTAILGIDGK
                     GEYATTFFGYGENGKIHKIKEFFDPDSLGGLYGAITEFLGFEMLDGEFKVMGMAPYGD
                     ASKYDFSRLAKFENGELVINTEYANVIGLRRYKENGKGFYFSKKLIEWLGPKRQGDIA
                     DDPYIHYAASMQALFEKLALQMMEHYLGDIIRETGKIAFAGGCALNVKLNQKIIARDD
                     VKELFVQPASGDAGTAVGAAAYVSHQRGVPVEKMEHVYLGPSYSNEDVIAACARHPSK
                     PVFKRIDNMPQRIAKIMVDANPVAWFQGRMEFGPRALGGRSIIGCPSVPGVADRINEQ
                     IKFRERWRPFCPSMLDTVGPQMLKVDHPSPFMTFTFEVNEEWKTRVGEVVHEDGTSRA
                     QVLKREYNPRYYDMMLELEKLTGNGVSLNTSLNRRGEPMICSPTDALNMFYGSDLQYL
                     IMEDILVVKDGKDWYDNV"
     gene            complement(23611..25071)
                     /locus_tag="VFF22_05960"
     CDS             complement(23611..25071)
                     /locus_tag="VFF22_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010656267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HZX16393.1"
                     /translation="MKLAELSQAGRAPELPLTIELADAAGSAELTLQRLLRVLPGQRY
                     VAQAEWRGRPVLAKLLVGDKAARHFQRERDGARLLASQGLNTPLLLAEGLRNDEGGWL
                     LFEYLEGAQSLWDAWRAVEHQPLLSSDRQAVLADALISIAQMHAKGLWQADLHLDNLL
                     RHNGQLFVIDGGGVQVETTGKPLSRGKVLENLGVFFAQLPAELAPFIEELLVHYLLAN
                     GEHALPLEALLKEVEKVRKWRLRDYLRKVARDCSLFAAKIGAFGLQVVRRDNQVELQP
                     LLNSLDLLTEQGHIYKTGGAATVAQVQMQGRPLVVKRYNVKNFGHGLKRFWRPSRAWH
                     SWVEGNRLQLLGIATPQPLAVLERRWCWLRGRAYLITEYCGGQDIIARFQPYQDGTPP
                     ETELLALDRLFAALLRERISHGDFKGHNLFWDESLGRWSLIDLDAMQQHRSAHSFAKA
                     YARDRARFLRNWPADSALYQLLNQRIPLTPAVGSAD"
     gene            complement(25120..25854)
                     /locus_tag="VFF22_05965"
     CDS             complement(25120..25854)
                     /locus_tag="VFF22_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017700158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HZX16394.1"
                     /translation="MAGWTLDDQYQELSADFGSLDAVFALRGEQVTKDSMSDVIRIER
                     AGVRYYVKRYCAAGKGLRSFIGRPRVKAEWQNLKHFAKWDVATAPIVAYGLERKQGQF
                     VRGALITRELPHTQDLALIANCDDPRLRDRAWVEVVSVQLAQATRRLHDHHFTHNDLK
                     WRNLLVDDQARLFLIDCPTGSFWWGPLLRYRIIKDLACLDKVAKYKLSRSQRLRFYLR
                     YRDRASLNDSDKRRIRQIVRFFEGRE"
     gene            complement(25854..26660)
                     /gene="rfaP"
                     /locus_tag="VFF22_05970"
     CDS             complement(25854..26660)
                     /gene="rfaP"
                     /locus_tag="VFF22_05970"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HZX16395.1"
                     /translation="MKLVLAEPFKTLWAGRDAFAAVEALQGQVYRELEGRRTLRTEVD
                     GRGYFVKIHRGIGWGEIVKNLLTAKLPVLGARQEWDAIQRLTEVGVATMTAVAYGERG
                     NNPAAQHSFIVTEELAPTTDLEQLSLNWAEQPPEPRLKWALIKEVASMVGNMHRAGVN
                     HRDCYICHFLLHTDKPVTVDDFRLSVIDLHRAQVRSQLPLRWRNKDLAALYFSVLDIG
                     LTRRDKLRFLRSYFQQPLRQTLSVERSLLAWLERKAEALYARKQRYGDAL"
     gene            complement(26657..27778)
                     /locus_tag="VFF22_05975"
     CDS             complement(26657..27778)
                     /locus_tag="VFF22_05975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZX16396.1"
                     /translation="MQLAFILYKYFPFGGLQRDFMRIALECQARGHAIRVYTPIWEGE
                     VPAGFDVVVVPVKALFNHRRNEKLTAWVEADLAKRPVDRVIGFNKMPGLDVYYAADGC
                     YEDKAQTLRNPLYKRWGRYKHFSDYERAVFAPESKTEILMISEVQQPLFIKHYHTPQQ
                     RFHLLPPGIAADRRAPANAAEIRAEFRAEFKLADSDLLLVQIGSGFKTKGLDRSLKAL
                     AALPRELKQRTRLIAIGQDDPRSFQLQAKALGVSDQVQILKGRSDISRFLLGADLLIH
                     PAYNENTGTVLLEALVSGLPVLVTDVCGYAHYIAEADAGRVLSGPFEQERLNHLLADM
                     LADDQRRAFWGRNGLAYADSADLYSMPQHAADIILAARP"
     gene            complement(27778..28779)
                     /gene="waaC"
                     /locus_tag="VFF22_05980"
     CDS             complement(27778..28779)
                     /gene="waaC"
                     /locus_tag="VFF22_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020305780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HZX16397.1"
                     /translation="MRVLLIKTSSLGDVVHTLPALTDAARAIPGIQFDWVVEEGFAEI
                     PAWHPAVAQVIPVAIRRWRKHLWQTFKNGEWKRFKQRLGETQYDLVIDAQGLLKSAWL
                     TRYVKAPIAGLDRDSAREPLASRFYDHAYAVPREQHALERTRQLFAQALGYTLPAGVG
                     DYGLNRAQLADPAPQPYLLFLHGTTWPSKHWPEADWRALAEQMNALGWAVRLPWGNEA
                     EKARAERIAAGIENAAVLPKLNLAGVAKVIAGASACVAVDTGLGHLAAALDVPSISLY
                     GPTLPGRVGAYGRGQVHLCATGPNAGCGDRHKPCFDGLDAERVAAQLQALLRAEDVV"
     gene            complement(28781..29815)
                     /gene="waaF"
                     /locus_tag="VFF22_05985"
     CDS             complement(28781..29815)
                     /gene="waaF"
                     /locus_tag="VFF22_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017336199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HZX16398.1"
                     /translation="MKILIVGPSWVGDMVMAQTLFQCLQQRHPGCAIDVLAPEWSRPI
                     LERMPEVRQALSFPLGHGVLELATRRTIGKSMAGQYDQAILLPNSLKSALVPFFAGIP
                     KRTGWKGELRYGLLNDIRTLDKEHYPLMIERFMALAFEPGASLPQPYPRPALQIDAQS
                     RDAALAKFGLSLDRPVLALCPGAEFGEAKRWPSEHYAKVAELKIRAGWQVWIFGSKND
                     HPVGEDIRSRLIPGLREEAVNLAGETSLAEAIDLLSCAGAVVSNDSGLMHVAAALNRP
                     LVAVYGSTSPGFTPPLAEQVEIVRLGLECSPCFDRTCRFGHYNCLGQLKPRPVIEALD
                     RLVSDPVEVE"
     gene            complement(29927..30850)
                     /locus_tag="VFF22_05990"
     CDS             complement(29927..30850)
                     /locus_tag="VFF22_05990"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HZX16399.1"
                     /translation="MSMADRDGVIWYDGELVPWRNATTHVLTHTLHYGMGVFEGVRAY
                     NTPQGTAIFRLQAHTDRLFDSAHIMGMKIPFSKDEINEATRAAVRENNLESAYIRPMV
                     FYGSEAMGLRASGLKTQVIVAAWSWGAYMGDEALQVGIKVRTSSFTRHHVNISMTRAK
                     ANGNYINSMLALQEAISGGADEAMLLDPEGYVAEGSGENIFLVKNGVIYTPEVTSCLN
                     GITRNTILTLAAEHGLQVIEKRITRDEVYIADEAFFTGTAAEVTPIREVDGRQIGAGR
                     RGPITEKLQTAYFDLVTGKTDGHAEWRTLVK"
     gene            complement(30936..33884)
                     /gene="glnE"
                     /locus_tag="VFF22_05995"
     CDS             complement(30936..33884)
                     /gene="glnE"
                     /locus_tag="VFF22_05995"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016972664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HZX16400.1"
                     /translation="MSLPALASLPAALSPLALRAEQSLQQAFAGLSGEAAAAFAGWPS
                     ERLAVWQRIAAASDFVVQQGARDPQMLLALAASGELERSLGAGELRGQLAQVLEDCAD
                     DDELGRRLRRFRNRQQLRIIWRDISRQADLIETCRDLSDLADACIDLAYQWLYPRHCT
                     QFGTPIGRRSGQAQHMVILGMGKLGAHELNLSSDIDLIFGYPEGGETEGVKRPLDNQE
                     FFIRLGQKLIKALDVITVDGFVFRTDMRLRPYGSSGSLVFSFNALEQYYQDQGRDWER
                     YAMIKARVVGGDQAAGAQLLEMLRPFVYRRYLDFSAIEALRAMKQLIQQEVRRKGMAE
                     NIKLGSGGIREVEFIAQAFQLIHGGRDLSLQQRSLFAVLNTLRDQGYLPPAVTDELRD
                     GYAFLRYAEHALQAIDDRQTQMLPDNDQDRARVAFIMGFDSWQAFHEQLMHWRGRVDW
                     HFRQVIADPDEDEGGEPVEEAVGCEWLPLWDDVQDEEAACRQLAEAGFAEPQSAWRRL
                     AGLRNGNQVRAMQRLGRERLDAFIPRLLAQAVEHDNPDLVLERVLPLIEAVARRSAYL
                     VLLTENPSALQRLLTLCAASPWIAEQITRFPLLLDELLNEGRLFKPPQAPELAAELRE
                     RLMRIPEEDLEQQMEALRHFKLAHRLRVAASEIAGTLPLMKVSDYLTWLAEAILDQVL
                     ALAWRHTVSKYGAPRRPDGSICDPDFVIVGYGKVGGIELGHGSDLDLVFIHDGDPHAE
                     TDGEKSIDGAQFFNRLGQRIIHLLTTQTNSGQLYEVDMRLRPSGAAGLLVSSLGAFER
                     YQQGEAWTWEHQALVRARVLVGCARVGKAFEAVRAGVLGRERDLATLRSEVSEMRAKM
                     RDNLGSKATAAGTAANAFEATAPFDLKQDAGGIVDIEFMVQYAALAWSRQYPELLEFT
                     DNIRILEGLERVGLLPGEDARLLQDIYKAYRSAAHRQALQKQPGVVSGDQFAEERRTV
                     MRIWQGLGLS"
BASE COUNT         7762 a        10791 c         9379 g         6252 t
ORIGIN      
        1 tgcacggcaa aggcatcctg atcaagaagg ccctggccag cggtcacgcc tgcctggcgc
       61 cgggtgtcga cccggtgcgc gccagtttcg agctgatctt cggccacccg ggcgcctgca
      121 gcgcgatcat cggcaccatc aacccgcagc acctggcgca taacgtcgcc accgccgccg
      181 ctgtaattca ggatattgcc taagcgttca cgcccgctca tctgtgccaa gctctcaggc
      241 cgtaaccatg cccgcgagga gttgccatgc cgcgcaccct gatccgcaaa gacccgagca
      301 acttcaagac tctgcagctg tttgtcgacg ccagcccgga aggcctgagc taccagggac
      361 tgggcatgcc actgaacttc gcgcagatgc tcgagaagcg caaaccggta aacgtggccg
      421 acacccagcg ctttgccgtg gagctggcca acctcggcgt ctcggtgcgc ctgactctct
      481 gctggcaggg ccgcgaatac tgggtgctgg tgcgccagcg ccgcgctgat cgcggcgacg
      541 tggtgctcaa gctgatctcc ggttacgtgc cggcccacga actcaatctg ccgctgctca
      601 ctgctattca ggaagtggcc gaagaatgcc tactggaaac cccggaaggc tggctgagcg
      661 ggcgctttgg cgacacctgg ctaccgacgc cctaccagag cagcctgcgc taccgggaaa
      721 ccgcgcactt tagcctcagt tcgctgtccg gcgcggcgcg gccggtgcag tgcggcaacc
      781 ttaagctgct cgaacgcccg cgcgcctacg tgcacctgcc gaccgcctca ctgcagctgg
      841 tctacgacct gcgcctggag ctgcccaagg acacgcgcca gctcagcctg ctgcatgtcg
      901 atgaacacct ggaagatggc caactggtcg cccgcctcaa ccgcgcgcgt cccgacctgt
      961 ttctgattcc gctggaccag ggtcggccga ccgccgagct gctgaccctg aagcagggcc
     1021 agctatcacc cgccagcacc cgcggcctgt ggctggccga aagctttgcg ccgcagggtg
     1081 gctggctggt gcgcgatgag cgcattcgct ggaaggattg gctggtgcag caaaactgcg
     1141 ccgatccggc cgcaaagaat agccgggcac aaaaaagccc cgcctagcgg ggccaaacgg
     1201 gatttattgc aggcgcttat ttcaactcgc tggaactctt gccgagcaag cgccttgcga
     1261 cgatcagcag ctggatctgc tgggtgcctt cgaagatgtc gagaatcttc gagtcgcgcg
     1321 cccacttctc cagcagttcg tcctcgccgt agcccatggc tccggccagc tccacacact
     1381 tgagggtgac ctcgttggcc acgcgcccgg ccttggcttt ggcaatcgac gcctccttgg
     1441 aattgggcag cttgttgtcg gccatccagg cggctttcat ggtcagcagg cgtgcagctt
     1501 cccattcagc ttcaaggcga tacaaggtcg cctcggcatg gctgacgctg agcaacggcg
     1561 tgcggtaatc cagcttgcag accttcttca gcagttcgcg agcgcggtcc agcgatgcct
     1621 tggctacgcc aaccgccata ccggcgacca gcgggcgagt gttgtcgaag gtttccatca
     1681 ccccggcaaa gcccttctgc acatcgattt ctggattgcc cagcaggttg gccgcaggca
     1741 cccggcagtc actgaagctg atcgaggcgg tgtccgaagc cttgatgccg agcttcttct
     1801 ccagccgcgt tacgctcatg cccggggtgc cgtgctcgac aacgaaggac ttgatcgccg
     1861 agcggccgag gttcttgtcg agggtggccc agaccaccac agaatcggca cgctcgccag
     1921 aggtgacgaa gatcttctca ccgttgagca cgtagtgatc gccgtccttg atcgcggtgg
     1981 tgcgaattgc cgcagagtca gagccacagc ccggttcggt aatcgccatg gctgcccagg
     2041 taccgccgaa gcgcgccagc tgctcatcat tggccaccgc ggcgatggcc gcattgccca
     2101 ggccctgacg cggcatagcc agcagcaggc cgacatcgcc ccagcacagc tccatcaccc
     2161 cgagtagggc ggacaggtta ccgccgttct tgacgccttc ctgctgctcg cgggtcgcac
     2221 cacgcttgct cgccgaggtg gcgccgatgg catccggcga gccggcgttc atgccgtcga
     2281 gcagggccgc cagcaggtcc agttctttgg gataggcgtg ctcggccttg tcgtatttgc
     2341 gtgaaatagg acggaaatag ttctccgcca cttgatgggc ctgattgacc aagccacgga
     2401 atttcttggg ggtttctaag ttcattggct acgcccttag agctggaggc cgccggccat
     2461 aatcgccagc gcacgcaaat cgcggtacca gcgctcggct ggatgttctt tggtaaagcc
     2521 atgaccgccg agtaattgca cggcgtcggt gccgattttc atggccttct cgctgcacag
     2581 caggcgcgcc agataggcct cgcgctggaa cgacaagccc tgctcggcac gcgagcacgc
     2641 gcgccagacc attaggcgca tggcatcgag ttcaacggcc atgtcggcga ccataaaggc
     2701 cacgccttgg cgatggctga ttggctcacc gaaggccacg cgctcgttgc agtaaggcac
     2761 tacataatcc aacgccgcct gcgccgtgcc cacagccagg gcgcaccagg ctaggccggc
     2821 ataatcgaga aaactctggt agctgaaaca ctcagcggcc aggcgcgcct cagccggcac
     2881 cttaacgcca ttgagccgca cacgcgcggt gctgcctgcc ttcaggccca tagccggctg
     2941 cgggctgcgc ttcagacctt tggcgccggc ctcgacaata aacagcgccg ggccatccgc
     3001 cgcctgtgca gcgatgatca actggctggc atccaggccg cgcaacacca agcacttctc
     3061 accactgagc acgtaatgct tgccgcgctt gcgcgcccga gtcgccagtt tgtgcggatc
     3121 aaacaacggc gtcggttcat tgaccgcgat ggccattacc gccgccggct cctcaccgac
     3181 aaacgccggc agccaacgcg cctgctgcgc ggcactgccc caacgacgaa tgcagttagc
     3241 cgccgacagc ggcaccaaca aggccgcagc caaggacaaa tcgcccttgc ccagcgcttc
     3301 ggcaatcagc gcattgctca gggtggtgcg ctcgcccgcc ataccgccct gggcttcgct
     3361 caccgcgtag tgagtcagcc ccagttcatg ggcctggtta agcaaggcag caggcagcgc
     3421 agcctgctcg tcggcatcat gggccagcgg acgcagcact tcgccagcaa aggcttcaag
     3481 catctccacc agcatctgct gctcatcgga cagcgacaga tcgaacaagg catcggggtc
     3541 aaccgggcgc ggaagcctgg cgactttgcc cgcacgttgt gcagcagcac ggaaactggc
     3601 gcggctgccg gtgtagataa gtttttcgaa gggcttgcgc agtttaaaac ggtcaggcca
     3661 gtctgcctgg gcgactcgat tcagcactgc cagggcacgg ccctgtacgt cggatgtcat
     3721 ggcatctcac tccatgcggg ggatgccgat aatcatgcgc gcggtcggag caagcgacta
     3781 tgaccgcgcc gacgccagcg actggcacaa cagccagtcg ctcagcgaag gaaaaccgtt
     3841 ctgaacatgg gaacaagcaa acggttaccg aactcggtga agtggtcagt atcgcgatag
     3901 agcgggcgat taccctcggc actcggacac ttatcgtcgc tacagaggta gggcaacggg
     3961 ttcagcagat gcacaccgca gcgctttgct gtctcatctt gcactgccca gataaaggca
     4021 tggcgcttgt ggtaatccgc cagagacaga ctgaaacggc gctccctacc cagtagcatg
     4081 gcgcgcccga taagcgtcgg tacatcgaca cccatttccg gggtcgggcg cacaatgtag
     4141 accggccgat gttcggccag gctacacatg gtcgtaacat atcgctcacg gaactgctca
     4201 agaaactctg cactgaactg ttcgacgggc tcgccaaagt gaaaggacgg cttagtagtg
     4261 gtcgacaaac caccctgcac atacttggag gtgcgaccca tcatgacgat cggaacgcca
     4321 gccgggaaac tggcatgctc cttttccaaa tcagcattga gcttttcgca gtagtgtttg
     4381 tcgctgctgg tagtttgcat gccgaaggcg atcaaacagc cactgccgcc gcggaacagc
     4441 acgccaccct cttcattagg caaagccgcc tgcaaggcag tcgccaccgc gtcggcatgg
     4501 ctatcaccaa tcaggatggc gcgtacaggc tcttgaccgt aaatgcagga ggcagcaggg
     4561 tcgaggcaat cctccaagcg agggttgtga ttcttgcgct ccgcttcgac gatcgcgacg
     4621 gcctccggca aacgatcagg aaacccactg cgccgcactt gttgcgccac gatggcaacg
     4681 agcgccagta ccactacaac ggcaatccca gcacggcctg cggaaagcag actcaggcgc
     4741 tggcgcgccg gtatttcgac cagcacgtaa gacagatgcc ccagaaacaa cgatacagcg
     4801 atggcggagg caatccaaag cgggtcgctc agcaactcca ggtaagccag cgccactacc
     4861 aagggccagt gccacagata gatcgaataa gagcgcgtac ccaaccactg ggcaggaaaa
     4921 ttggctgtcc acattgaatg tccgcgcgca gccaacagca ccaacgcggc accgacgacg
     4981 gggagaatgg cccgccagcc aggccataca ctttgcttgt cgaggagcac gatggtggcg
     5041 aggatcaaag cgaaacccgc taattccagc gccctgcgca gaccatcagg caatcctcga
     5101 tggccaaaca taaataccag tgcaccggct agcatctccc aggcgcgagt ctgcagcata
     5161 tagaaggctt cactgggcgc caccgaggta cgccacaggc acagtgccag agaaacaacg
     5221 aagagcagcc ctagcacaat cgtgatcgca cgccagccag gcaggaactt gctaacgagc
     5281 atcaacacca gcggcaggat caggtagaac tgccactcca ctgacaacga ccaggtatgc
     5341 agcagccact tctcctgaga agcaacatcg aagtaaccgg actcttcgaa gtagcgcagg
     5401 ttggagctga atgtgaggct ttcacgggcg tgcttaccca acagcttgta ctcttccggc
     5461 atcaggaaga accagccaaa gatcaacgcc accacgcaca gcacaatcag cgccgggaat
     5521 atacgccgag cccgcgccag gtagaaacgc cacaccgaga actcaccacg cgcgatacct
     5581 ccgctgatga tgccgcacat caaatagccg gaaatgacga agaacacgtc aaccccagca
     5641 aaaccgccgc ttatacctgt taccccgaag tgatacagca ccaccgccat cacggcccaa
     5701 gcacgcagcc cattgatatc ctgccggaac tcttgcgcta ctgcgccctc cactctactc
     5761 accaaccaaa ctccacggta taaagggcac ctacgggcac catattcttt tcgttatatg
     5821 aactagcgac tccagcgaat acacggcggc cttcctcgaa agccttggtc accagcgagc
     5881 aaggccgcac tatgctttcc ggctgattgc tagccacgtc gatgaacgaa tgcttgtcgc
     5941 cggtaccgat cagaatatcg gtggaaatca cacagtcgtt gccgatatcg atacttgcag
     6001 cctcgtacct gaaaataccc ccctccaccg tgttgtcggc cccgatgcgg acatgctgac
     6061 cgtgggtatg cattaggtag attttgccga agctggacgg aggcgcgtgc ggatctcaat
     6121 cagattgcct tcgccatcaa gacgaatctc acagtgactg acctccacgc cctcaccgaa
     6181 caccaggcag tttctgcaag ccagattgcg cagcgaaagg tcaagatggt cgagcatggt
     6241 tcaatcctgg ctgcggattt tctcgacgat ggcggtggtc gagctgtttt ccaccagccc
     6301 cagcacccgc acttcgccgc cataagcggt gacgatatca gcgccgacca cctgctcgat
     6361 gccatagtcg ccgcccttga ccagcacatc cggtttgacc gcacgcaaca ggttctccgg
     6421 ggtgtcttcg ctaaagctca ccacccaatc gaccgcgcct aagccggcca gcacggccat
     6481 acgccgatcc accgcgttga tcggtcgacc cgggcctttc aggcggctga ccgaggcatc
     6541 gtcattcact gccagcacca gtcggtcgcc ctcggcgcgc gcctgctcca ggtaggtcac
     6601 gtggccagca tgcagaatgt cgaaacaacc attggtgaag acaatcttct cgccatgggc
     6661 acgggcatct tcaatcgcca acaacagttg atcgacgctt agcacgccac gctcggagcc
     6721 cgcttcacgc tgaatcgcac ggcgcagctc gggtgcgctg atagcggcgg taccgagctt
     6781 gccgaccacg ataccggcag ctaggttggc cagagccacg gcatgcggca gctcctcgcc
     6841 ggcagccagg ctggcggcca gggtagaaat caccgtatcg ccagcaccgg tcacatcgaa
     6901 cacttcgcgt gcgcgcgccg gcagatgcag cgccggctgg tctgggcgca gcagggtcat
     6961 gccatgctcg ccacgggtca ccagtagcgc gcccagctcc agctcgctca tcaacgccgc
     7021 gcccttggcc accaactcgg cctcatcacg gcaagggccg acgatggttt cgaattcgtg
     7081 caggttgggg gtaatcaggc tggcgccacg atagatggag aaatccttgc ccttgggatc
     7141 ggccagcacg gcaatgcctt tgctaagggc cagctgcacc agtgtctgat gattctgcag
     7201 cgcgcccttg ccgtaatccg agaggatcag caccttgatg ccatccagtt gcgcttcgac
     7261 actggcggcc agcgcggcac tgtcggtgcg gaatggctct tcgaaatcca tgcgcagcag
     7321 ctgttgatga cggctcatca cccgtaattt gacgatggtg ggctgatctt tgatcgtttg
     7381 gaaaaaggtc tttacaccaa ccgcctgcag gctgttgcgc agactttcag cggcttcatc
     7441 ctgcccagtc acgcccagca atgtagctgg cgcgccgagg gcggcgatgt tgagcgcaac
     7501 gttggccgca ccacccggac gatcctcgat ttgctcaacc ttgaccaccg gcaccggcgc
     7561 ttccggagaa atccgcgagg tgccgccatg ccaatagcgg tcgagcatga catcgcccac
     7621 caccagaacg gcggcctggt cgaaacgcgg catggacaat ttcataaaca ctccagaagc
     7681 ggctaaaacg aatcgccgca aatcataaca ttggcaggta aatacgctga tcaaccgtcg
     7741 agcgtacgca cccagaacag ttcgtggcgg cgcaccgcct tgcggaagaa ttcatcttca
     7801 ccggtggccg gccagcgccg cccgaccagc aggcgctgga tcaaccggcg gatgcgtcgt
     7861 ttaagcggca atgcgccttg taaatcgtgc tgcatggcca aggccatggc cttatccagc
     7921 tgccgcgcgg catccaatcc agggctccac aggccatcag ccggcagcgc ttcaatagct
     7981 ggcggcagtg caatgccaaa gcccgcaggg cccattggcc agcggtcgtt gtcgtgcaga
     8041 tggttggcat acagcagcag ggttttcggc ttccagctgc tcagctcaac cgcctgcagc
     8101 agcgcctcgg tgctcgccac atgatcactg tgcgggtcca gctgcgggtg cggcgtgacc
     8161 accacctcag gctggaagtg ctcaagcgcc gccactaaat cggccagcag attgcaccaa
     8221 ctgggcaggc catcagcatc gccaggcagt tggattgaat tagcggcgcg cacgctgcgg
     8281 gtatcaccct cgcccgactc gcgcgaagca aaagccgcct cgggttgcgc ctgcatcgca
     8341 ggcaactgca ggcagtaata gcccagctgc aaacactggc tctgcggcac gccgccccag
     8401 agtggaactg ccaagctgtc ccacgcacgc agacgcccct tgagtcgcgc ggcggcggcc
     8461 tgatctagcc ccagcacctg atagtactcg gcctcgattt cgccctgggt cagggtcacg
     8521 atgctgacat cacgcgcccg gctgtacagc ccaaacgctg ccaattcggc gtcgtcggca
     8581 tgcggcgcaa tcaccatcac gcgctgctgg gcataatccg gattgctcag ggcaaacaga
     8641 gtgacggccg gctcaatacg gcagaagcga ccgcgcagct gtagcgtgcc cgtggccaat
     8701 gcatcttgct gaccagacag gttgaggtaa cgacagccat tcacgccccg ctcgaaatcc
     8761 tgacggtcag tgccaatcag cacatagggg tccagccagc gccctagcca gctggccttg
     8821 agttgcacct gcaaaatcag agtatcggca gcgccaatat cgccatccag caacaggcgc
     8881 cctgcctcga tgcgccctgc taatgcaggc acatcctgag caaacgcata gctgtagtcg
     8941 tcatccgggg cgtaaaacag atggtcggca aaccatgcct catgggctat ccagcccacc
     9001 agcaacagca atggcaccca ccaccaggcg gcaaatatac ccagcagaca taacagcacc
     9061 agcgccacca acagtgcaat gcgcttttgc cgacgatgct gcttgagcag ggtttgcttg
     9121 cgtgcagaca tattcagacc tgataaaccg gcatgcgatt gcaccagcga tctttgtatt
     9181 cgcggtcggc ccgcccaaac gaatagcgca gcggtttgcc gatagcccgc gcttcagccc
     9241 aggctgtttg ggtgttgata aagctgagca cgctgccggg gctgaactcg cgattttgcg
     9301 ggtccacacc accattgatg tattccaggc tgacccactg cggcgactcg actcggtaga
     9361 gcacttgaat agccactggc tgatcattca ggtagatcag cgagccagtc ataaagtcgc
     9421 gcatcaggct gaacacctcg gccaaattcg cctgcccggt cgcctcgaag ccccagcggc
     9481 gctgaaacag atcggcgtat atcaccgcct gctcggcagc ggacaactcg agcatcggtc
     9541 gtacaacacc gcccgcctct tccagcaagc gctgctcacg gcgctggttg tagcgaaact
     9601 tcttgctgta ctcctcaggc tcgcgggcca acgccaggcc ttcgggctgc tcacgtcgac
     9661 cgatcacctg ctcggcattc agagccgaca ggtagcgcac cttgtggcgc agcggtacgc
     9721 gcgcgtcgct tgccagcggc aggatgacct cggcattgcc caaatcgaat aagccacgct
     9781 tgccctgctg tttcagtaca tccttggaca acgccagatg ccgcccccag gtcggcaccg
     9841 cagcgcgtaa ctccccccgc tcaaaccagc ccaggtaacg caccggcaag cctgcgagcc
     9901 ctaccagacg ctctataacc tgcggatggg tagcgacact gccaccaaac cgctgccagg
     9961 catcagcata ggcggcagca tcaatcagcg tccaaccccg ctcacgccag acgcgtaaat
    10021 gactgagcat caggcttcgg cctcaggcat gctcgattcg acggcctcga caaaatcctt
    10081 ctcatgcagt cgtgcgtaat agccgttctg cgctaacagt tgcgcatgat tgccgcgctc
    10141 gacaatccgc cccttgtcca tcaccagaat cagatcggct ttctcaatcg tactgagacg
    10201 atgggcaatc accagagtgg tacgcccctt catcacctca tccagagcgg cctgaatatg
    10261 ccgctctgac tcggtatcca acgccgaggt ggcctcatca agaatcagca gcggtgcatc
    10321 cttgaggagt gcgcgggcaa ttgctaggcg ttgccgttga ccaccggaca gcagtacacc
    10381 gttctctccc accacggtgt cgtagccctg cggcatctga tcgataaact cggcggcata
    10441 ggctgactcg gaggcacgct tcacatcttc aagcggcgca cctgccagat ccccataggc
    10501 aatattgttt gccacagtgt cattgaacag cgttacgtgc tgggtaacca aggcgatatg
    10561 acggcgcagg ttgcgcagct tgtagtcctc aacatcaatg tcatccagca ggatctgccc
    10621 ctgatcgtgg tgatagaagc gtggaatcag attagccagt gtcgacttgc cgctgccgga
    10681 acggccgacc agcgcgacca tttgcccagg ttcggcaaca aagctgatgt catccagtac
    10741 aggcttttca ctgcctttgt actgaaagct aaggttgcgt acatccaacc gtccactgac
    10801 gcgctcgcgc tcttgagtac cgagatcaat ctcaggcgct tcatcgagct gctcgaaaat
    10861 actttcggcc cccgcgacgc ctttctggat ggtcgagctg acttcggaca gctgccggat
    10921 gggcttcggc agcaaaccgg ccagggtgat ataggccact agctcaccgg cggtcgcgtc
    10981 gccccgcaac aacagcacca gaaacatcag cccggccatc gcgctataaa tcaccaactg
    11041 cagactcggc gtatacaccg cgttggtttt gaccattttc agctgtttta ggcggttttc
    11101 ggtactggcc tgggcaaaac gagtctcttc ataaccttcg ccaccgaagc tgcgcaccac
    11161 ccgataacca tgaatggttt ccgaagcgac atgggtcaca tcacccattg ctacctgaat
    11221 tttcttgctt tgcttgcgga atttcttgct ggcacttacc accatcaaac cgattatcgg
    11281 caaaatggcg accatcacta gggtcagttt ccagttcatc agcagcaagg tggcgaacag
    11341 gaaaaccacc gtcatacctt cacgaatgac caccttgatc gcatcggtag ccgcgccggt
    11401 gaccatcgtg acgttgtagg taatacgcga aatcagatga ccggaattgt gattgtcaaa
    11461 ataacgattg ggtagggtaa gcaggttatt gaacagcaat acgcgcatgt catgcactaa
    11521 acccagggaa actttggcca gatagtagtt acctagaaac gaggaaatgc cctgcagcaa
    11581 agcaatcaac acaatgagca gtggcaccgc ctgtaacaac ttcagctcgc ccagccacgg
    11641 catatccttc aaatagggaa cagctgcgaa gaaattggca ttgggattat ttagcccatc
    11701 aacaaagaac ttcagcatgt agccaagcat cggctgggtc acagcgaata gcataaaacc
    11761 caagatactg acagcgaaca gcccccagta agggataacg tagcgcagca acctcagata
    11821 cactttcaca ctggatggct gggtagaatt ggccattcgg caagccccat acgactagca
    11881 agagaaacaa gatgcggatt gtaacagcgc aggaactgga aagctggcta gccagcggca
    11941 cggtactgga aaaagacagc cgaggcccga aggtgctgat gttgagcgat ggccagatac
    12001 tgaaaatatt tcatacccgc agccaccctg tgttcagccg actgaaccca ccagccaaga
    12061 agtttgcgca aaatgcattg actctgcagc aactgggaat ccccgcaccg cagattttcg
    12121 agagcctgtg gattgacata gccgccggca tcagcgcatc gatttaccag ccactaccag
    12181 gccagtcggt tgaatcaata ttgaaagaaa cgccagagca aattgatgca ctgataccgc
    12241 aacttgctaa gtttataaaa acgctacacc gacaaggcgt gtactttcgc tccctgcacc
    12301 taggcaacat tctacaacta caaaacggcc aatttggcct gattgatttt ctcgatttaa
    12361 ccaagaaatc gcgatcgctg agcagctggg aagtaaacag aaacttcaag caccttgagc
    12421 gttacctgat gcggcgtaag cttatgagtt ttccttacaa aaaacttatg aacacctact
    12481 ccaagctata actgcagaaa ttacgaaaaa cgcttaacta gcaaagtata cacatagtgt
    12541 ttgtgttcag cctgccgagc agaaagaaat acacagtaga aaaacacaaa ggaaaaccac
    12601 agcatgtcga acgtacagcg cgtggagaaa ctggggaaaa cgcgttcaac cattgagttc
    12661 agcgcttccg cattcaataa ctctcccttt aatacagagc ccaacgcgag atcaaagcct
    12721 ggcggataaa aaccaaaatt atcccagtca agaacaattg agcccactcc catattcggc
    12781 aagctcagat caccatgccc gacaaacctg ggcatagcct cgcaacgctc cttcatcttg
    12841 agcagcactg agttcttttc gccaatttgc tcaagcactg cctgagtttt gcgcaagcat
    12901 cgctcaaaac cgaaatgtaa ctctagcgac ttaaattcac ctagaactaa cgtagagtca
    12961 tactcaaacg atgccagacg ccgagtgacc gacacaagac ggtcaatgta atcactctca
    13021 tcaatagcgc gaaaatcaac gaattcaaac ctggaaacaa ctaagcgctc gccagacacg
    13081 acatctaaca acctcggcac actcagctca tcaccgctca gcgccgaaag cacatgcttg
    13141 tagaaaaaat tgcatttacg ccaatcaacc gagtcccgca aatatatctt ctcgaacaac
    13201 acagagtctt gcagaacaat ttttctatac gaatttaacg tgtagcgacc tttacccgag
    13261 ccgacaaaat cactctctaa cgctccccgc ctgaagctga gcgctgcatc gtaagcactc
    13321 aacacacgct catacgcttc atggtccatc tgacgggcac agcgatcagc atatgtcttt
    13381 atatgcgcaa agccttgccg acctataaaa gccattcgga ttttatttat tatatcgagc
    13441 aaggcgcagc cccctgacaa acacaaaata ccgctgacta ggccaacaaa tcatcaagct
    13501 gattgtattt aatttttttg attacattgc taggtaaacg gctatcaaca gataagttga
    13561 acacttgaaa actgtcactt agtacgcgcc gtgacatcag ttcaaaactt ggcagaatgt
    13621 agtctgcata gtcatcgcct aagcgactag gcacctgcaa tccctgcgga tcatagaact
    13681 gcccctgttc tgggctcata tcaacaccaa ccagatacac ccgatcaaaa cctaggtgat
    13741 acgctagctg caaggcagca taagcgatgg tgcgcccatt gaaatagcca ttacacaagt
    13801 ttcgactaaa gccaatacgg ttacgttttt gccgaaacaa cgaccactcg acagagaact
    13861 ctgggtcgtt tcgaaccgac caagcaaagc gtctgtcgct aatggcagcg ctcccacgct
    13921 gacgattaac cctctccagc agatgcagct tgccagtaga taaggcatgt ggactgtgct
    13981 ttgccacttc ctgcaacgcg gccaaaccca atcccgaatt ttcggcgaga gtgatgcctt
    14041 gcgctactgc cgcgcctttg cgcttagcta cgccgcgatc atcacacaag taaaaaagcg
    14101 gcttgatacc ttcttcaata aaacgcgcga tagaaccatt catcgcgatc atcggaatat
    14161 ggctataacg ctgcattgga aacgtcaaca ctgaaggccc tgacgccacc aagaagaccg
    14221 caccactgaa ccgtccgcga ttttcagcga agtcgtgcaa agcaatgcac tgaccactgt
    14281 ccagttgtaa caatgaacta tcgacaacat cagaacacga catcagcgca cgccatcctt
    14341 gctgcaagcc aaagcaagcc cgagcggaaa ccacacccac aaccagacca gattaggaga
    14401 gtgtataaat gagtacagat caaaaaacat tacgccaatg caaccaacca gcaagcagag
    14461 gcctaaaaca gccatctcag agcgctctag ccagagctgg cgcagagcca acaacaaact
    14521 gctaataaaa agggccaagc cgacaatgcc atactgaaac aacacttcga gatagaggct
    14581 atgcgtattg acaacttgct gaccaggaat agacatcggc tgcatgctag agccagcccc
    14641 atgacctagc caccaatagc ctgacatgct actcagcacc atattccaaa tcaaatgccg
    14701 cccagtagcc ccgcgctcaa gctcaacact aagaatttcc ggaaaacgcc agagcaccaa
    14761 agacacgaaa agcgcgaatg tcaccccaat tgccaggaga aaataatcgc gtttggcctg
    14821 aaaaacaaat agaacagcac cacctaaccc catcgccacc caagcggttc gcgagaatgt
    14881 taaaaacaca tatagaccaa ttaccaacag gcaaaaaaac cataaactct tggcccaaac
    14941 tttattagta cgaagcacac accagagcgc cgtcaaacca gcaaacgcca tatacaggcc
    15001 agaaataatc ggattaccaa actgcgcaaa acctgtcact cccgagttag acaatcgaaa
    15061 cgcacgatac ttgaagtcca acccacgcgc tctgagctga taaattaacg tgacaaaagc
    15121 ggccacggcg gcaacaacaa caacaaccat taatagttgc cgcaaccaat cagtacgggc
    15181 gtctttaagc acgacaaccg cacacacaaa caccatcaac acgatgcttc gacgcaacac
    15241 accagagtcg ccgccaacca gtaaaaaatt cacaatcacc caagagacta agagcccagc
    15301 cgtcaccaac aatgctttgg ccttaaagcc tttacctatc tcacctgaga gcaaataagc
    15361 aattaaaaaa gataacaatg caaaccgcgc actaccggcc caagcagatg cctcaccggg
    15421 ccaccaaaag cgcccaagaa atatgatagc gagagataac aacgccaaca ttggcgtata
    15481 gggtcttact aactggaaca ccatcgataa ccgacctttc aaagccttag cggcaaactt
    15541 attaaatgat ttacggctta ttcagccatc aaagtaccta aagactctcg gaccgccacc
    15601 gggttagctg aaaaatactc aggccaacct ttaaattcgc tccaaccact gagaaagaca
    15661 aagtcaattc ctgcggcagt agcgcattga tggtcgaaac gactgtcacc gagaaacaac
    15721 gccggcatac ggatattccc tcgttccagc tctcgcgcca gaatcatatc cttagcatct
    15781 ggactaccgt agatgccgcc atcgaacaga gcggccaatc cacgcactgc aaagacctcg
    15841 cgcagctctt cttgatcgcc cccagaaacg ataagccagt ttgcatcagg caactgcgca
    15901 cgcaactcgt gcaagcctgg cgcgattgcg caactgcgca aaccgtcatg caccagagcg
    15961 gcataatgcg ctagcaaagc atccagagcc tccgagtttt tcggcaaccc gagaatctgc
    16021 ccgagcaagt actcaaactt gcgatagcgg gaaataccgc cattggccac atggtaatcc
    16081 actaacgcct gggccgccgc ttcgccatag ggcagcgcgg cctgataaaa cgcttcagtt
    16141 ttaacgtgat tggagttcag tacgacaccg tcgcaatcga ataccaggct gcaatactct
    16201 gtgagcaggc gcattacaga ccccgctcgc agatagctgc ttctgcccgt gctacatcat
    16261 ctggattatc gacagcgatg gaatctgcag acatttccag catgcgcacc tcccagccca
    16321 gctcgagaaa acgcaatatt tcaatatcct ccagcgcttc caagggtgtc ttgcgcggct
    16381 gttcggcaaa cgctacgagc gccgcccggg ggaaggcgta gacacatacc tgccgccagg
    16441 cgcgcacgaa cgcatgctgt ttgttgcccg gaataggtga gcgggacata tacaacagtc
    16501 gtccatctgg acgcatcacc accttgggaa tgctctcgct acgaaacgat tcatcgttat
    16561 ctattgcaca aaaaccattg aggacctcgc cagggtactg actcaaactc tccagaatcg
    16621 cacgcaggtc ctgcgggttg aaaaccggct catcgccctg cacattgatc aacacctctg
    16681 tgtccagttg ccgagcgcac tcggcaactc gatcagtgcc cgtcaggcag tccggtgacg
    16741 tcatcagcac ctgaatctcg tgcgctcggc aaacggcggc aatccgctca tcatcagtgg
    16801 ccacataaac ccgctcgggc ggacaggcct ggatacattg ccgataggtg cgtaccacca
    16861 tgggcacacc gcacagatcc accagcggct tgcccggcag ccggctcgat tggtaacgcg
    16921 cggggatgat caccgcgtaa tctggagcgc tcaaaattgc cagcctcaaa gcacgtaaat
    16981 agaagtacgg ggcacctgat agcggctagg tgtgaccgcc agcagatcgg gaacacccgg
    17041 caactgttgg aaagcatcaa acagctggtt gacctcaata ccgcgaggat catcagcggc
    17101 atagccgtca aaacccgcca gcagcactcg cgtggcctgg ccactggccg caatcgccaa
    17161 agcataggca gcgaccagtg acgtaggcag aacacagtgc tgctcagcaa aggcaaactg
    17221 cccggcctgc acggcgatgc cgaaatccaa cagtgttttg tctgccaagg cttcacgtac
    17281 gtcggcaggc agcatatgtg cgggggtcac caagggctgc ggcagcttgc gatgttcctg
    17341 gcagtcagcc atcaagcgga aaggatggca ggccgcacgc acatcaatca gttcggcagc
    17401 caggtgatcc tgagtattga gtgcaataac gaacggcttg gcccggcgaa tatagtcctc
    17461 cagcccctga cgatgacgcg cgacgccagg cccgctgccc agcagcagta cttcccggcc
    17521 agcgatcagc gtctgcgggg cccagttgcc acggggctcg ccactgtaga aatgacgcgc
    17581 cgactccagc gtcccagggc tgtatttctt gccgccctga tgcttgagga aatcaatgac
    17641 aaccagcagg tcttcctcgg cataacgtga gtcagtcaac atttcctgaa tgtaggtggg
    17701 atggatgccg taagtgcctg ccaggaaata atagggattg gtgccccaac tatgacgctg
    17761 ctgcagcggc tggaagtgac gagcaatcac attgagcagc ggcgccacct gcaccgagcg
    17821 cgcgcgcttg gccatcaatt cgactactaa gtactcggtg cgcacattgc ccggcccgcg
    17881 ccccatacct gtcaccgtgc tgtccagcca gagcacacca tcctctactg cgcgcagggt
    17941 attggccaag gccatgctgc gattgtcgtg ggtatgaata cccagggcac catgccagcc
    18001 acggcgcagg gcctggataa tggcactggt ctgatcaggc gaaagactgc ccatgctgtc
    18061 agcgaaatac agtacatcaa gcgcatgccc ctcggcctgc cgggcaagat gctcgatttc
    18121 ctcgggctgg cgatctgcta tctgcatcag attgatgccc actcggtaac cttgcgcctt
    18181 cagccagtcg catgcgggca gggccgcctc aaactcatgc acatggcaag caatacgcac
    18241 caaatcgaca ggggagtccg cagcaggtgc gaacaacaac tccaaggccg gcaaaacccc
    18301 tgcggggtgc ttgatcagct cgcttgcatt gatcatcacg ccaatgcccg gaccttccgg
    18361 caattccagg cgccgcagga agctgtcggt ggtgtaggca taagccccac gaaaaccatt
    18421 actctcgaag ctgcgcaaac ccagctcgac atagtcgacc gcgaccgcag ccatagcccg
    18481 caggtagtcc tgcaccagct cggcatcaaa gtcccagttg ttgtagtacc cgccgtcacg
    18541 gagagtacag tccagcatga ccaaagcttg gttgctcatt ccatcgtcct cgacggctca
    18601 gtcaaatgag ccggtggtaa gcaaacgcct tcgcagtgcc ctgtgcgttg caactgctca
    18661 agcaattgca gattacgcag caacccctga ctgccctgag ccagatggta atcggcaaat
    18721 cgctcagccg caccgacgca atcggcatag ccggcctcaa tctcgagcac acgttgctgc
    18781 aactgctcga catcggtaat gcaggcgcca aagaactgct cctgggaaaa aatatcctgc
    18841 tgctcacccg cattgacata gaccactggc cgacgggcga gcgcagcctc aatcacagca
    18901 ttcgacatga tattgatcac taccgatgcc tgcttcaacg catcggccag accgcagtca
    18961 cgagggagaa cctcgacctg agtcgagcgc gcagccgcct catgccagaa cggcaccgca
    19021 ctgcgcgggt gcgccttgat caacaaccgg taactcgggt tctgcagcgc ccaggcttgc
    19081 aacaacgcgt aggtacgctg atacccgtgc tccttttcct tgtccggccc aactccgagc
    19141 accagcatca ctcgacgatc cgggtcagat gctggcaggt cgaaggcgcc atcgatcatg
    19201 tgtgaacctg ccaacaccgc agtcgatgcg ccaaaccgca acgcacgcgc ctgcaaggct
    19261 tcaagtgaac tctggccgaa gagaaaatag tagtcgtagt cgttcatccc caatcggcta
    19321 gaaccctcca ctgtcgtggc atgagccagg tgcaccaaca gagcgtcgcg ttgattcaac
    19381 gccatacgca ggaatggcgc atataaactg ccattacgat cattgagcag aatcctgggc
    19441 tggtagcgcg ccatcaacca ctgcacatgg gcggcgtaac cgaaatagcg caacggcacc
    19501 ttatacagcg gcgcgcacag caagcgctgc cgcacgataa tcgacgcctc tggcaatgct
    19561 gtttcgatca acacatggcc gcgttcgcgc agtgcctgca tcagcagttt cttgcgctga
    19621 aaagcgatca ccttgggggc agattgcagc agcaagaaat cacaggtttc gccttcgaca
    19681 acctgagcca ggctcagccg cgcacgcact ccgaacagca gatcgctgag catttcacgg
    19741 gcgaagcgca gcgcactacc taatacgcca tgacgctcac gcagtaaaca ccaacggtaa
    19801 cgatccagct cgcgggcctt gcgcatgacc tcctggctca cggcgtagcg ccagggtgaa
    19861 agcctttgcg atcgacatgc caagactggc tgctcgccag tgcttgagcc tgctcaaggg
    19921 tcaacggtgg ctcatcctgc ttgtgccact gttttttcca gaccagaaaa tgcaggtaag
    19981 ggaattcgcg cgccccgtcc ttagcattgg tcaggctacc gggagcccat gtccagtact
    20041 ctggataatc ttgagtgccg tcagtccagg cacgcacgcc gtttggcgta ctgaaggctt
    20101 ccttgaacct ggcaacccga cgccaacggt tgaacttacc gaccaatttg aacagcggtt
    20161 tgggaaaatt cttgcgccag agaaacagac gactgaaagc cccctcatcg agcgcgtgat
    20221 tcttctgatc ctgcactcgg cgctgaaaat ctggaatttc gcggaacagc tcacgtagcg
    20281 gctgctcgtt tcgcagcaga cacaggtggc cggaaacccg ccgctcatgc gtcgagaaga
    20341 acttataacg acgcagccgc tcgtcgcgaa aataagcccg caagttaccg tagatcagat
    20401 ccaggtcgct aaagccccag tagtcatacc cggccacatc ctgttcatgc aggtagccca
    20461 atgccggctt gagatcgcag agtttgtagg ggctcttggg tgcaaaagcg aaacccaaac
    20521 gttccgatat ccacgcgcag taatccgcaa aactgatatg ccgctgctta acattcggcg
    20581 gcaggttatc cagaggcgcg caatcgccca ccagcaacca gtcaatactt ggattacccc
    20641 ggcagctctc gacgaaaaac ggcatccaga atggccactg gccaaagtag ggaataagga
    20701 aaatgatgct cggctgctta ttcattactg actgaactcc agaaaacgcc agaaccgtgg
    20761 ccgggcgacc tcatcgacat attccttgcc cagtttcagc aactgtccct gacgcaacgc
    20821 tgcaccagtc gccgtatcca gcttgctcag ggacaccaat cctgcggcca aagcaccggc
    20881 atcagccagc gggaatagct gagcaacacc ttgcgccacc tcccgcccgc caccgcaatc
    20941 actgcagata accggtacgc ctgccgccat cgcttccagc aacaccatgc cgaatggttc
    21001 atggtctgag gtcagggcaa acacgtcaaa agccttgaag tagcgccgcc cataagcgac
    21061 ctggccaaga aagcggacct gttcgtcaac gttaagctcc tgcgccagcg ctttgagcgc
    21121 aggctccagg ccgccactgc ccataattgc cagcagactg ccagccggca attgcggcaa
    21181 ggcctgggcg aaacctttga tcagcgtcgc ctggtctttg tcgggatgca ggcggccaac
    21241 attgccaacc acccaggcgt tctggggcaa cccgagatgc tcgcgagctg cttcacggga
    21301 aacctgctcg gcttgcactg cgacaatatc aatgcggttg tacagcgtct cgatacgctc
    21361 ggccggccaa ttcggcaagc aggcgcgcat atcatcacgt acagcattgg aaacccccag
    21421 caaagccagg cgcttgcgaa agaggttggc gaacagctga cgcgtacgcc gcttgtagtc
    21481 cccaaacgca tggtgcacac cgatcaccgg tagatcgctg cccagcaggg cgacgtagat
    21541 aggtttgaag cgatgggcaa tgcagaattt gaagtcgcgc gaggcggcaa tgcgcttgag
    21601 atcacggatc gccttgagct tgaggccacg cacttgccga ctggaatagt cgagaaaaat
    21661 cacctcgtca gaggccgagc cttgctcgac ttctgcactc ggtttgccag tcagatagac
    21721 cgtacacact ttgtaaggcg taccgacaaa tagcgcagcg tactgccgcg cacagtcgag
    21781 gaaggggccg tcataaccgt ggcagaactg caaaacccac ggctgaccag ccccagcaac
    21841 ctcagacatt gtcataccaa tccttgccgt ccttgaccac gaggatgtct tccatgatca
    21901 ggtactgcag gtcggagccg tagaacatgt tcagcgcgtc ggtcggagag caaatcatcg
    21961 gttcgccacg gcggttcagc gaggtattca gcgacacgcc gttgccggtc agtttttcca
    22021 gctccagcat catgtcgtag tagcgcgggt tgtattcgcg cttgagcacc tgggcgcgcg
    22081 aggtgccatc ctcgtgcacc acttcaccga cgcgggtctt ccactcctcg ttaacttcga
    22141 aggtgaaggt catgaacggg ctcgggtgat cgaccttgag catctgcggg ccgacggtgt
    22201 cgagcatcga cgggcagaac ggcctccagc gttcgcggaa cttgatctgt tcattgatac
    22261 gatcagccac gcccggcacg ctggggcaac cgatgatcga acgcccgccg agggcgcgtg
    22321 ggccgaactc catgcgcccc tggaaccagg ccacgggatt agcgtcgacc atgattttgg
    22381 caatgcgctg cggcatgttg tcgatgcgtt tgaacaccgg tttgctcggg tggcgagcgc
    22441 aggcggcgat cacgtcttcg ttggagtagg acgggccgag gtaaacatgc tccatcttct
    22501 ccaccggtac gccgcgctgg tgcgacacat aggcagccgc gccgactgcg gtaccggcgt
    22561 cgccggacgc cggttgcacg aacagctcct tcacatcatc gcgggcgatg attttctggt
    22621 tcagcttaac gttcagtgcg cagccgccgg caaaggcgat cttgccggtc tcgcgaatga
    22681 tgtcaccgag gtagtgctcc atcatctgca gcgccagctt ctcgaacaac gcctgcatgc
    22741 tcgccgcgta atggatgtaa ggatcatcgg cgatgtcgcc ctgacgcttc ggccccagcc
    22801 actcgatcag cttcttggag aagtagaagc ccttgccgtt ttctttatag cggcgcaggc
    22861 cgatgacgtt ggcgtactcg gtgttgatca ccaactcgcc attttcgaac ttggccagac
    22921 gcgagaaatc gtacttgctg gcatcgccgt aaggcgccat gcccatcacc ttgaactcgc
    22981 cgtcgagcat ctcaaaaccg aggaattcgg tgatcgcgcc gtacaggccg ccgagggaat
    23041 ccggatcgaa gaactcctta atcttgtgga tcttgccgtt ctcgccgtag ccaaagaagg
    23101 tggtggcgta ctcgcccttg ccgtcgatgc cgaggatcgc ggtcttctcg gtaaaacccg
    23161 aacagtggta ggcactggcg gcgtgggcca ggtggtgttc aaccggctcg atcttgatct
    23221 tcttcaggtc aaagccgagc tgctgcaggc accactggat gtgcttgtag tagcgtttgt
    23281 agcggcggtt gcccatcagg atcgcgtcga gggcgcggtc cggcgcgtac cagtaacgct
    23341 tggcatactg ccagcgggcc ttctcgaaaa tactgatcgg cgcgaagggg atcgccacca
    23401 cgtcgacatc agacggcttg atcccggcct gctccaggca gaacttggcc gactcgtagg
    23461 gcatgcggtt cttcgcgtgc ttgtcgcgca cgaagcgctc ttcttcgacc gccgcgatca
    23521 gcttgccgtc gatatacagg gcggcggaag ggtcatggct gagggcgccg gacaggccga
    23581 gaatggtcag tgccactggt tttgcctctt ctagtctgct gagccgacgg cgggcgtcag
    23641 cggtatacgt tggttgagca actggtacag cgccgaatcg gcgggccagt tgcgcaggaa
    23701 acgggcacgg tcgcgggcat aggccttggc gaaactgtgc gcactgcggt gctgctgcat
    23761 ggcgtcaaga tcgatcaacg accaacgacc caggctttca tcccaaaaca gattgtgccc
    23821 cttgaaatca ccatggctga tgcgttcgcg cagcaaggcg gcgaacaaac gatccagtgc
    23881 caacagctcg gtttccgggg gtgttccatc ttggtatggc tgaaagcgcg cgattatatc
    23941 ctgcccaccg cagtattcgg taatcaggta ggcacgcccg cgtagccagc accagcgccg
    24001 ctctagcacg gccaatggct gcggagtggc gatgcccagc aactgcaggc gattgccttc
    24061 gacccaactg tgccaggcgc ggctgggacg ccagaagcgc ttcaacccgt ggccgaagtt
    24121 tttcacgttg tagcgcttga ccaccagcgg ccggccttgc atctgcactt gtgccacggt
    24181 ggccgcgccg ccggtcttgt agatatggcc ctgctcggtg agcagatcca ggctgttcag
    24241 cagcggctgc agttcgacct ggttgtcgcg acgcaccacc tgcaggccga acgcaccgat
    24301 ttttgcggcg aacagactgc aatcgcgggc gactttgcgc agataatcac gcaaacgcca
    24361 ctttcgcacc ttttccactt cctttagcaa cgcttccagc ggcaacgcat gctcaccgtt
    24421 ggccagcagg taatgcacca gcagctcttc gataaagggc gccaactcgg ctggcagctg
    24481 ggcaaagaac accccaaggt tttccagcac cttgccgcgc gacagcggct tgccggtcgt
    24541 ttcgacctgc acaccaccgc catcgatgac aaacagctgg ccgttatgcc gtaacagatt
    24601 gtccagatgc agatcggcct gccacaaccc cttggcgtgc atctgcgcga tgctgatcag
    24661 cgcatcggcc agcaccgcct gccgatcgga gctgagcaac ggctggtgct ccacagcgcg
    24721 ccaagcgtcc cacaggctct gcgccccctc caggtactcg aacagcagcc aaccgccctc
    24781 gtcattacgc aaaccttcag ccagcagcag cggcgtattc agcccctgcg aggcgagcaa
    24841 acgcgcacca tcacgctcgc gctgaaaatg ccgcgcagcc ttgtcgccaa ccagcaactt
    24901 ggccagcacc ggccgcccgc gccattcggc ctgggcgaca tagcgctgcc cgggcaatac
    24961 gcgcagcagg cgctgcaggg tcagttcagc cgagcccgct gcatcggcta gctcgatggt
    25021 cagcggcaac tctggcgcgc ggccagcctg actcagctca gcaagcttca cgcagcagat
    25081 ccttaatcat ggaatggggc atagcagtgg acaggcggct cattcgcgcc cctcaaaaaa
    25141 tctgacgatc tggcgaattc ggcgtttatc actgtcattt aatgaagctc ggtcgcgata
    25201 gcgcaggtaa aagcgcaagc gctggctacg cgacagtttg tacttggcga ccttatccaa
    25261 acaggccaga tccttgataa tccgatagcg caacaacggc ccccaccaga acgagccagt
    25321 cgggcaatcg ataagaaaca gcctggcctg gtcatcaacc agcaagttgc gccacttcag
    25381 gtcgttatgg gtgaagtgat gatcgtgcaa cctgcgggtg gcctgcgcca gctgcacact
    25441 gaccacctcg acccaggcac ggtcgcgcaa gcgaggatca tcgcaattgg caatcagcgc
    25501 caagtcctgg gtatggggca attcgcgggt aatcagcgcg ccccgtacaa actgcccctg
    25561 cttgcgctcc agcccatagg cgacgatcgg ggctgtagcc acgtcccatt tggcaaaatg
    25621 cttgagattt tgccactcag ccttaacccg tggtcgcccg ataaaactgc gtagcccctt
    25681 accagcagcg cagtagcgct tcacgtaata gcgcacgcca gcgcgctcga tgcggataac
    25741 atctgacatc gagtccttgg ttacttgttc accccgcagg gcaaacaccg catccagact
    25801 accgaaatcc gcagacagct cctgatactg atcatccagc gtccagcccg ccatcagagc
    25861 gcatccccat aacgctgctt gcgcgcatac agcgcctctg ccttgcgctc tagccaagca
    25921 agcagcgaac gctcgacgct aagggtctgg cgcaacggct gttgaaaata gctacgcaga
    25981 aagcgcagct tgtcgcgccg ggtcaggccg atatccagca cggagaaata cagcgcggcc
    26041 aggtccttgt tgcgccagcg caagggcagc tggcttcgca cctgggcacg gtgcagatcg
    26101 atcaccgaca gacggaaatc atcgacggtg actggtttgt cggtgtgcag cagaaagtgg
    26161 cagatatagc agtcgcggtg gttgaccccg gcgcggtgca tgttgccgac catactcgcc
    26221 acttccttga tcaacgccca cttcagccgt ggctcgggcg gttgctctgc ccagttcagg
    26281 ctgagctgct caagatcggt ggtcggtgcc agttcttcgg tgacgataaa ggaatgctgc
    26341 gccgccgggt tgttgccgcg ctcgccgtag gccacagccg tcatggtcgc cacaccgacc
    26401 tcggtcaaac gctgaatggc gtcccactcc tgacgcgccc cgagcaccgg caatttggca
    26461 gtgagcaggt tcttgacgat ctcaccccaa ccaatgccgc ggtggatctt gacgaaatag
    26521 cctcgcccgt cgacttcggt gcgcagcgtg cgccggcctt ccagctcgcg gtacacctgc
    26581 ccctgcaacg cttcgacagc ggcaaaggca tcgcgcccgg cccatagggt tttgaagggt
    26641 tcagccagta ccaacttcat ggacgcgccg ccaggatgat gtcggccgcg tgctgcggca
    26701 tggaatacag atcagcgcta tcggcatagg ccaggccgtt tcggccccag aaggcgcggc
    26761 gctgatcgtc ggcgagcata tcggcgagca gatggttaag gcgctcctgc tcgaacggcc
    26821 cactcaacac ccgcccggca tccgcctcgg caatgtagtg ggcatagccg cagacatccg
    26881 tcaccagcac cggcaagccg gagaccagcg cctccagcag cacggtgccg gtgttctcgt
    26941 tgtaggccgg atggatcaac agatcggcac cgagcaggaa acgtgaaata tcgctgcgcc
    27001 ccttgagaat ctgcacctga tcggacacgc ctaaagcctt ggcctgcagc tggaacgaac
    27061 gagggtcatc ctgaccgatg gcgatcaacc gggtgcgctg cttcagctcg cgcggcaagg
    27121 cggccagggc cttgaggctg cgatccaggc ccttggtctt gaagccggag ccgatctgca
    27181 ccagcagcag gtcgctatcc gccagcttga actcggcgcg gaattcggca cgaatttcgg
    27241 cggcattggc cggcgcgcgg cggtctgcgg cgatgcccgg cggcagcagg tgaaagcgct
    27301 gctgcggggt gtggtaatgc ttgataaaca gcggttgctg cacttcggag atcatcagaa
    27361 tctcggtctt cgactcgggg gcaaacaccg cgcgctcata atcggaaaag tgtttgtaac
    27421 ggccccagcg cttgtacagc gggttgcgca gggtctgcgc cttgtcttca tagcagccat
    27481 cggcggcgta gtagacgtcc agaccaggca tcttgttgaa gccgatcacc cgatccaccg
    27541 gacgcttggc caggtcggct tcgacccagg cggtgagctt ttcattacgt ctgtggttga
    27601 acagcgcttt gaccggcacc accaccacgt caaagcccgc aggcacttca ccttcccaga
    27661 tcggcgtgta gacgcgaatg gcatgaccgc gcgcctggca ctccagggca atacgcataa
    27721 agtcgcgctg caggccgccg aaggggaagt atttgtagag gataaacgcg agctgcatca
    27781 gacaacatcc tcggcccgca acagggcctg caattgtgcg gcgacacgct cggcatccag
    27841 gccgtcgaag cagggcttgt gacgatcacc acagccggca ttcggccccg tggcgcacag
    27901 gtgtacttgc ccacgcccat aggcaccgac gcgacctggc agggtcgggc cgtacaggga
    27961 aatactcggt acatccagcg cagcggccag gtgacccagc ccggtatcga cggctacaca
    28021 agcgctggcg ccggcgatca ctttcgccac gcctgcaaga ttcaatttgg gcagcaccgc
    28081 cgcattttca atgccggcgg caattcgctc ggcacgggct ttctcagcct cattgcccca
    28141 gggcaggcgc actgcccacc ccagcgcatt catctgctcg gccaacgcac gccagtcggc
    28201 ttcaggccag tgcttgctgg gccaggtggt gccgtgcaga aacaaaaggt agggctgcgg
    28261 cgcagggtcg gccagctgcg cacgattgag gccgtagtca ccgacaccgg caggcagcgt
    28321 gtagcccagc gcctgagcaa acagttgacg ggtacgctca agagcatgct gctcgcgcgg
    28381 cacggcatag gcgtgatcat aaaagcgact ggccagtggc tcgcgggcgg aatcgcgatc
    28441 cagtccggca atcggcgctt tgacgtaacg ggtcagccag gcacttttca gcagaccctg
    28501 ggcatcgatc accaagtcgt actgggtttc accaagacgt tgcttgaagc gcttccactc
    28561 accgttcttg aacgtttgcc acaggtgctt gcgccagcgg cgaatggcca ccgggataac
    28621 ctgcgccacg gctgggtgcc aggcgggaat ctcggcaaag ccctcttcca ccacccagtc
    28681 aaactggatg ccggggatcg cccgcgccgc atcagtgagc gccggcaagg tgtgcaccac
    28741 atcgcccagc gaagaggttt tgatcagcaa tacccgcaag ctattcaacc tcaaccgggt
    28801 cgctgaccag gcgatccagc gcctcgatca ccgggcgcgg cttgagctgg cccaggcagt
    28861 tgtaatgacc gaaacggcag gtgcggtcga agcatgggct gcattccagg cccaggcgga
    28921 cgatttccac ctgctcggcc agcggcgggg taaagcccgg cgaagtcgag ccgtagaccg
    28981 ccaccaacgg gcggttcagc gccgcggcca catgcatcag gccagaatcg ttggacacca
    29041 cagcgcccgc gcaggacagc agatcaatgg cttcagccag gctggtttca ccggccaggt
    29101 tcaccgcttc ctcacgcagg ccgggaatca gccggctgcg aatatcttcg ccaaccgggt
    29161 gatcgttctt cgagccgaaa atccacacct gccagccggc gcggattttc agctcggcga
    29221 ccttggcgta atgctcgctc ggccagcgct tggcttcacc aaactcagcc cccgggcaca
    29281 gcgccagcac cgggcggtcc aggctcagac cgaacttggc cagtgcagca tcgcggctct
    29341 gcgcgtcgat ctgcaaggcg gggcgtggat aaggttgcgg cagcgaagca ccgggctcga
    29401 aggccagcgc cataaagcgc tcgatcatca gcgggtagtg ctctttatca agcgtgcgga
    29461 tgtcgttgag caggccgtag cgcagctcac ccttccagcc ggtacgcttc gggatcccgg
    29521 cgaaaaacgg caccagcgca gacttcagcg agttgggcag cagaatcgcc tgatcgtact
    29581 ggccggccat ggacttgccg atcgtacgcc gcgtggccag ctccaacacg ccgtgaccga
    29641 gcggaaaact cagggcctgg cgcacttcgg gcatgcgctc gagaatcggc cgactccact
    29701 cgggggccag cacatcgatt gcgcaaccgg ggtgacgctg ttgcaggcac tggaacagcg
    29761 tctgcgccat caccatgtca cccacccagc tggggccaac gatcagtatt ttcatgccac
    29821 atccagaaac gaccaaggag gctttcgcct cccggacaga ctcaacgctc gactatcagc
    29881 ctagcctgcg ggccgaatgc aggagcatcc agcccatgcg aagcagttat ttgaccagcg
    29941 tacgccactc ggcgtggcca tcggttttac cggttaccag gtcgaaataa gcggtttgca
    30001 gcttctcggt gatcggcccg cgacgacctg cgccgatctg ccggccatcg acttcacgga
    30061 tcggcgtcac ttcggcggca gtgccagtga agaaggcttc gtcggcgata tacacctcat
    30121 cacgggtgat gcgtttctcg atcacctgca gaccatgttc ggcggccaga gtcagaatgg
    30181 tattacgggt aataccgttc aggcaggaag tcacttccgg ggtgtagatc acaccgtttt
    30241 tgaccaggaa gatattctcg ccagaaccct cagccacgta gccttccgga tccagcagca
    30301 tggcctcgtc ggcaccgccg gagatggctt cctgcagggc cagcatcgag ttgatgtagt
    30361 tgccgttggc cttggcgcgg gtcatcgaga tgttgacgtg gtggcgggtg aaggagctgg
    30421 tacgcacctt gatgccaacc tgcagggctt cgtcgcccat ataggcgccc cagctccagg
    30481 ccgcgacgat cacctgggtt ttcaggccac tggcgcgcaa gcccatggct tcagatccgt
    30541 agaacaccat cgggcggata taagcgcttt ccaggttgtt ctcacgcacg gcggcgcggg
    30601 tggcttcgtt gatctcgtct ttgctgaacg ggatcttcat gcccatgatg tgggccgagt
    30661 cgaacaggcg gtcggtgtgc gcttgcaggc ggaaaatcgc cgtgccttgt ggggtgttgt
    30721 aggcgcgcac gccttcaaac acgcccatgc cgtagtgcag ggtatgggtc agcacgtggg
    30781 tggtcgcgtt gcgccacggc accagctcgc cgtcatacca gatcacgcca tcacgatcgg
    30841 ccatcgacat agttgcctgc tcctcaaact caattggtac aacattggcc ggccaccctg
    30901 ggcgcccgac atcctcaaat tcagctcagc ctgcttcagc tcaagcccag tccctgccag
    30961 atgcgcatca ccgtgcgccg ctcttcggca aactgatcac cgctgaccac cccaggctgc
    31021 ttttgcaggg cctgacggtg ggccgccgag cggtaggcct tgtagatatc ctgcagcaag
    31081 cgggcatctt cacccggtag caagccgacc cgctccagcc cttccagaat gcgaatattg
    31141 tcagtgaact caagcaattc cgggtactgg cgcgaccagg ccagggccgc gtattgcacc
    31201 ataaattcaa tatcgacgat acctccggcg tcctgcttga ggtcgaacgg cgcagtggcc
    31261 tcaaaggcat tcgccgcggt gccggccgcc gtggctttgc tgccgaggtt atcgcgcatc
    31321 ttcgcgcgca tctcgctgac ttcgctgcgc agcgtggcca ggtcgcgctc gcgccccaac
    31381 accccggcgc gcaccgcctc gaacgccttg ccgacacgcg cgcaaccgac cagcacccgc
    31441 gcccgcacca gcgcctgatg ctcccaggtc caggcctcgc cctgctgata acgctcgaag
    31501 gcgcccagcg agctgaccag caggcccgct gcaccggaag gccgcaggcg catgtcgact
    31561 tcgtagagct ggccggagtt ggtctgggtg gtcagcaggt gaatgatgcg ctggcccagg
    31621 cgattgaaga actgcgcgcc gtcgatggac ttttcgccat cggtttccgc atgtgggtcg
    31681 ccatcgtgga taaacaccaa gtcaagatcc gaaccgtgac ccagctcgat gccgccgacc
    31741 ttgccgtagc cgacgataac gaaatccgga tcgcagatgc tgccgtcagg acgacgtggc
    31801 gcaccgtatt tgctcaccgt atggcgccag gccagggcca gcacctgatc gagaatcgcc
    31861 tcggccagcc aggtcaagta gtcactgact ttcatcagcg gcagggtgcc agcaatttcc
    31921 gaagcggcaa cgcgcaggcg gtgcgccagc ttgaagtgac gcagcgcctc catctgctgc
    31981 tccaagtctt cctcgggaat acgcatcaag cgctcgcgca gctcggcggc cagctcgggg
    32041 gcctgcggcg gcttgaataa acgcccttca ttaagtagct catcgagcaa cagcgggaag
    32101 cgggtgatct gctcggcaat ccatgggctt gcagcgcaga gggtcagcag acgctgcaac
    32161 gcgctggggt tttccgtgag cagcaccaga taggccgaac gccgcgccac ggcctcgatc
    32221 agcggcagta cgcgctccag caccaggtcc ggattgtcat gctccaccgc ctgcgccagc
    32281 aggcgcggaa taaaggcatc caggcgttca cgaccgaggc gctgcatggc acgcacctga
    32341 ttgccattgc gcagccccgc caggcgccgc caggccgatt gcggctcggc aaagcctgcc
    32401 tcagccagct gccggcacgc ggcctcttcg tcctgcacat catcccagag cggcaaccac
    32461 tcgcagccca cggcctcttc gaccggctcg ccaccttcat cttcatcggg atcggcgatc
    32521 acctggcgga agtgccagtc aacccggccg cgccagtgca tcagctgctc gtggaacgcc
    32581 tgccagctgt cgaagcccat aataaaggct acacgcgcac ggtcctgatc attgtccggg
    32641 agcatctgcg tctgccggtc atcgatggcc tgcagcgcgt gctcggcata acgcaggaag
    32701 gcatagccgt cgcgcagttc atcggtcacc gccggcggca ggtagccctg gtcgcgcagg
    32761 gtattcagca ccgcaaacag cgagcgctgc tgcaggctga ggtcgcgacc gccgtgaatc
    32821 agctggaaag cctgggcgat aaactccacc tcgcggatgc caccagaacc cagcttgatg
    32881 ttctcggcca tgcccttgcg ccgcacttcc tgctggatca gctgcttcat ggcgcgcagc
    32941 gcttcaatcg ccgagaaatc caaataacgc cgataaacga agggccgtag catctccagc
    33001 agctgcgcgc cagcagcctg gtcgccaccg accacccgcg ccttgatcat cgcgtagcgt
    33061 tcccagtcgc gaccctggtc ctggtagtac tgctccaggg cgttaaagct gaacaccagc
    33121 gaaccggaag agccgtacgg gcgcagacgc atgtcggtgc ggaagacgaa gccgtcgacg
    33181 gtgatcacgt ccagcgcctt gatcagcttc tgaccgagac ggataaagaa ctcctgatta
    33241 tccagcgggc gcttcacccc ttcggtttcg ccgccttcgg ggtagccgaa gatcaggtcg
    33301 atatcagagg acaagttcag ctcatgggcg cccagcttgc ccatgccgag gatgaccata
    33361 tgctgcgcct ggccgctgcg ccgaccgatt ggcgtgccga actgcgtgca atggcgtgga
    33421 tagagccact gataagccag gtcgatacag gcatcggcca gatcggaaag gtcgcggcag
    33481 gtttcgatca aatccgcctg gcggctgata tcgcgccaga taatccgcaa ctgctgacgg
    33541 ttacggaagc gccgcaagcg tcgtcccagc tcgtcgtcat cagcgcagtc ctccagcacc
    33601 tgcgccaatt ggccgcgcaa ttcgcccgcc ccgaggctgc gctccagctc accgctggcg
    33661 gccagcgcga gcagcatttg cgggtcacgc gcgccctgct gcaccacgaa atcactggcc
    33721 gcagctatgc gctgccagac cgcaagccgc tcgctcggcc aacccgcgaa agctgcggcc
    33781 gcctcgccgg aaaggccggc aaaggcctgt tgcagggact gttcggcgcg cagtgcgagg
    33841 ggtgataagg cagcaggcaa agaggccagc gcgggcaggc tcatggtcta tcctttttag
    33901 cgatctgcca gtgcagcgat catggatggc accacccact ctagagcggg tcgaatcact
    33961 ggcagtgaaa taattgtagt tttactacca aagattgtat ccaaaaggct gaaatcggcg
    34021 tcggaatgta gtaaaactac acacagccgg tcctaccccc ggtcaatcca agaattcacg
    34081 cggccactca cgaagtcggc cacgaaacct ggcctccgat tctggaagcc tttccgccct
    34141 ggagcaagcc atgcaagacc tcgatcccgt cgaaacccag gaat
//
LOCUS       DASRUM010000089         3771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1388683, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000089 DASRUM010000000
VERSION     DASRUM010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388683"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(323..562)
                     /locus_tag="VFF22_06000"
     CDS             complement(323..562)
                     /locus_tag="VFF22_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HZX16401.1"
                     /translation="MKGQKGYALVSAGRLQGRRPFEILRAVGRQFSRWWQLSEQRRRL
                     AMLDDAALKDLGLSRGDVLQESERPFWDDPLGRGP"
     gene            660..1514
                     /locus_tag="VFF22_06005"
     CDS             660..1514
                     /locus_tag="VFF22_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZX16402.1"
                     /translation="MASYPSIDSELLRTFVAIADHGGFTRAAEMVNRTQSAVSMQMKR
                     LEDDVVQRPLFVRDGRQFSLTAEGQLLLGYARRILKLHSEVMTSMRAPHMVGTVRIGT
                     PDDYVMRFMPGILSRFAQAYPLVQVELHCEPSFQLLQRQDLDLTIVTREPGTEIGQLL
                     RQERIVWAEAQGFSPHEQEPLPLAMFNAQCFCRSWACNALDAMEREYRIAYTSPSLSA
                     IMAVVSAGLAITAQLQSLITPDMRIIGTAEGLPMMPSASIVLLRNSQTQSQVTETLAE
                     QIVEGFRL"
     gene            complement(1504..2253)
                     /locus_tag="VFF22_06010"
     CDS             complement(1504..2253)
                     /locus_tag="VFF22_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZX16403.1"
                     /translation="MLDFALNLLLGLALGTLGGLFGIGGGLIAIPALGVLFGLDQQLA
                     QGTALVMVVPNVLLALWRYHQRNRIDWRHALALAVPSLIFAVLGAGWAVTVDAERMRL
                     GFVVFLVLLAAYNLSRLYSKSAKPSVGLRYPWPWLTLLGSGAGLLGGLFGVGGAVLAT
                     PVLTTLFGTSQVMAQGLSLALAAPSTAVTLATYAAHDHVDWLLGAPLALGGLLSISWG
                     VKCAHALPERVLRGLFAGFLLLCAVLLALKV"
     gene            2366..3322
                     /locus_tag="VFF22_06015"
     CDS             2366..3322
                     /locus_tag="VFF22_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX16404.1"
                     /translation="MNPDSLTDQLSLFLDVLETGSFSAAARRHPLTPSAVARRVDALE
                     RSLGSNLFSRSTHAVRATSAGLAFAERARRILAELHLARAEAVSLSSAPEGLIRIDAP
                     APFGRRHLAPAIAEFLVAYPGLDVQLRLIDSFVDLHGTHLGEVDLVLRIVPLADTRLV
                     ATALSPMVRVLCASPEYLARCGVPRSPDELLEHDGLDWDSLAPPYAWRFEQDGKLQLC
                     RPKRLRMTANNAEALLFSALAGLGIAHLPTWLISDYLLRGELLPLFCENSLPAPEPSG
                     IYALRLERETDSRSRLLLEFLKARFGPVAPWDLALKSGLQRS"
BASE COUNT          795 a         1249 c         1037 g          690 t
ORIGIN      
        1 gaggcgaatt ctacagcgtt tcaaaccgct gtcaaccgcc tcttttaccg ctttcgatca
       61 acttgaccga accccttcag acttaaacac taccccctga agaggaggcg cattctacgc
      121 agctttcgct gctttgcaac ctctaattaa acctaacctc ttgttttata aaaattttat
      181 gaggcagcat acgcctgaag agctgcgcac cgtaggggct atataagtat acgtcgggat
      241 taaccctgta tttccggtca acttcagcct ccatcagtga gccccctaac cgcgcattgc
      301 cacagcatca caacttcgac ggtcatggtc ctcgacccag cggatcatcc cagaatggcc
      361 gctcgctttc ctgcagtaca tcgccacgac tcaagccgag gtccttcaat gctgcatcat
      421 cgagcatggc caagcgccgc cgttgttcgg acagctgcca ccagcgggaa aactgacgac
      481 caaccgcacg cagtatttca aacggccggc ggccctgtaa gcgtccggca ctgactaatg
      541 cataaccttt ttgacctttc atctcgtcgc cctccttgtg gggttggcga cagtctcgcg
      601 ctcggcttat gatcaatcca acgaatgttt cttatgcaat gcatctcgga gattgatgta
      661 tggccagcta cccgagcatc gatagcgaac tgctgcgcac ctttgtcgcg attgccgatc
      721 acggcggttt tacccgtgca gcggaaatgg tcaaccgcac ccagtccgcc gtcagcatgc
      781 agatgaagcg cctggaagac gacgtggtgc agcgcccgct gtttgtgcgc gacggccgac
      841 agttcagcct caccgccgaa ggccagttac tgctcggtta tgcccgacgc attctcaaat
      901 tgcacagtga agtgatgacc agcatgcgtg ccccgcacat ggttggcacc gtgcgcatcg
      961 gcaccccgga cgactatgtg atgcgcttta tgccgggcat cctctcgcgc ttcgcccagg
     1021 cttatccgct cgtgcaggtg gaattgcact gcgagccttc gttccaactg ctgcagcgcc
     1081 aagatctgga cctgaccatc gtcacccgcg agcccggtac cgaaattggc cagctgctgc
     1141 gtcaagagcg gattgtctgg gccgaggccc agggcttcag cccgcatgag caggaaccgc
     1201 taccgctggc gatgttcaat gcccagtgct tctgccgttc ttgggcctgc aacgcgttgg
     1261 acgccatgga gcgcgaatac cgcatcgcct acaccagccc aagcctctcc gcgatcatgg
     1321 cggtggtcag cgccggtctg gcgattaccg cgcagctgca aagcctgatc accccggata
     1381 tgcgcatcat tggcaccgcc gagggcctgc cgatgatgcc ctccgccagt atcgtgctac
     1441 tgcgtaacag ccagacccag tcgcaggtca ccgaaaccct ggccgaacag atcgtcgaag
     1501 gcttcagact ttaagcgcga gcaataccgc gcaaagcagc agaaaaccgg caaacagccc
     1561 gcgcagaacg cgctctggca gcgcgtgggc acactttacg ccccagctga tgctcagcaa
     1621 accacccagc gccaggggcg cacccagcag ccaatcgacg tgatcatggg ccgcgtaggt
     1681 cgccagcgtc acggccgtgc tcggtgcggc cagagccagg gacagcccct gcgccatgac
     1741 ctggctagtg ccgaacaacg tagtcagcac cggcgtcgcc agtaccgcac cgcccacacc
     1801 aaacaacccg ccgagcaagc cggcgccact gccaagcagc gtcaaccagg gccagggata
     1861 acgcaggcca accgagggct tggcagactt gctgtacagc cgagagaggt tataagcagc
     1921 cagcaacacc aggaaaacca cgaaccccag gcgcatacgc tcggcgtcca cagtgaccgc
     1981 ccagccggcg cccagcacgg caaatatcag gctgggcacc gccagggcca acgcatgccg
     2041 ccagtcgatg cggttgcgct ggtgataacg ccacagcgcc aacagcacat tcggcaccac
     2101 catcaccagc gccgtgccct gtgccagctg ttgatccagg ccaaacaaca cgccaagcgc
     2161 cggaatggcg atcagcccac cgccaattcc aaacaaaccg cccagggtgc cgagggccag
     2221 tccgagcaga agattcaagg caaagtcgag caaggtcatc gatagactcc agagtgaagc
     2281 tgcacagcct agttattcgc cactagccgc gaaacgcaca gcaacgcaaa atggctgtgc
     2341 atataacgca cagcagaacc taaccatgaa tcccgatagc ctgactgacc agctcagcct
     2401 gtttctcgat gtattggaaa ccggcagttt ttccgccgca gcccgtcgcc atccgctgac
     2461 gccctcggct gtggcccggc gcgtcgatgc actggaacgc tcactgggca gcaacctgtt
     2521 cagccgcagc actcatgccg tacgcgccac ctccgccggt ttggcgtttg ccgaacgggc
     2581 cagacgcatt cttgccgagc tgcacctggc tcgcgccgaa gccgtatcgc tgagcagcgc
     2641 gccggaaggg ctgattcgta tcgacgcgcc ggcaccgttc ggccggcgcc atctggcccc
     2701 cgcgattgcc gagtttctgg tcgcctatcc ggggctggat gtgcaattgc gcctgatcga
     2761 cagcttcgtc gatctacatg gcacccatct gggtgaagtc gacctggtgc tgcgcatcgt
     2821 ccctctggct gacacccgtc tggttgccac cgcgctatcg cccatggtgc gcgtgctctg
     2881 tgccagcccc gagtacctgg cgcggtgcgg cgtaccgcgc agccctgatg agctgctgga
     2941 acatgacggt ctcgactggg acagcctggc accgccctac gcctggcgct tcgagcagga
     3001 cggcaaactg caactgtgcc gccccaagcg cctgcgcatg accgccaaca atgccgaggc
     3061 gctgctgttc agcgccctcg ccggcttggg cattgcccat ttgccaacct ggctgatcag
     3121 cgactacctg ctgcgcggcg aactgctgcc gctgttctgc gaaaacagcc tgcccgctcc
     3181 cgagcccagc ggcatctacg ccctgcgcct agagcgcgaa accgactcgc gcagccgctt
     3241 gttgctggaa tttctcaagg cccgcttcgg cccggtggcg ccctgggacc tggcgctgaa
     3301 aagtggcttg cagcgcagtt aaagagcaga aacagcaggc ataaaaatga tcgggaatca
     3361 tttttagcgt cgcgcagcga cgacccgaag ggtggccacc atggatggca agccataaaa
     3421 aaggctctgt cccaagaccg tgttgcaagg attaagctta aggcaggcat ccgttcggga
     3481 ggtctgccca ccatggatac tcaagccttt cagcgctggt tggctcaggt gagccaactc
     3541 agcattaagc agaaatccgc actgcatcag gcgctacagg aaccacctac aacggaggtt
     3601 cttgatagct tgccagcgtt acagagctgt ccacattgcc aagcaaaagc tgagcacctt
     3661 gctccctggg gctggtcgcg tggcttacgt cgttatcgtt gcaagagctg ccggcggaca
     3721 tgctcagcgg tttcgagcag cggccttgct cgtttgcact atcccgaacg c
//
LOCUS       DASRUM010000090        51225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1388728, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000090 DASRUM010000000
VERSION     DASRUM010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 51225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51225
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388728"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(377..1189)
                     /gene="pssA"
                     /locus_tag="VFF22_06020"
     CDS             complement(377..1189)
                     /gene="pssA"
                     /locus_tag="VFF22_06020"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015096846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HZX16405.1"
                     /translation="MNERPDEPQSPADAESLLPIDEHIEEGHDAEGRKVRHRGIYLLP
                     NLFTTANLFAGFYAIINAMNGNFYVAAAAVFVAMVLDGLDGRVARLTNTQSAFGAEYD
                     SLSDMVAFGVAPALLAFEWALGSMGKVGWMVAFIYVAGAALRLARFNTQIGSVDKRYF
                     IGLASPAAAGVVAGTVWAFSDFGIKGSNMAFVVAILVAAAGMLMVSNIKYNSFKDLDL
                     KGRVPFVAILAVVLVFAVVFSDPPRILLIIFLAYAASGPIQYLLRLRRGKSA"
     gene            complement(1347..2363)
                     /gene="ilvC"
                     /locus_tag="VFF22_06025"
     CDS             complement(1347..2363)
                     /gene="ilvC"
                     /locus_tag="VFF22_06025"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HZX16406.1"
                     /translation="MKVYYDKDCDLSIIQGKKVAIIGYGSQGHAQACNLKDSGVDVTV
                     GLRKGSATVAKAEAHGLKVTDVASAVAAADLVMILTPDEFQSALYKNEIEPNIKKGAT
                     LAFSHGFAIHYNQVVPRSDLDVIMIAPKAPGHTVRSEFVKGGGIPDLIAIYQDASGNA
                     KNVALSYAAGVGGGRTGIIETTFKDETETDLFGEQAVLCGGTVELVKAGFETLVEAGY
                     APEMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEYGEYVTGPEVINAESRQAMRN
                     ALKRIQDGEYAKMFISEGATGYPSMTAKRRNNAAHGIEIIGEQLRSMMPWIGANKIVD
                     KAKN"
     gene            2489..3370
                     /gene="ilvY"
                     /locus_tag="VFF22_06030"
     CDS             2489..3370
                     /gene="ilvY"
                     /locus_tag="VFF22_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HZX16407.1"
                     /translation="MDSHALRLFLSLADNLHFGKTSREQHVSPSALSRSIKQLEDELG
                     APLFLRDNRSVRLTREGQQFREYASEVMNGWQAIRQTFKQDQLILHGELSLYCSVTAS
                     YSFLYDILSSFRHDYPRIEMKLHTGDAAKAVERVQEGLEDLAIGARPDSLPAGIEFQP
                     IARSKLCFIGPQSPQLLTEEQLKHPSAASWRDVPMILSEEDLARTRTDLWLKNHNIKP
                     LIYAQVSGNEAIVSMVSLGFGIGVVPQIVLDNSPLAARIRIYDIQPPLTGYEIGLFAL
                     EKRLKDPLINAFWNRQR"
     gene            complement(3460..3951)
                     /gene="ilvN"
                     /locus_tag="VFF22_06035"
     CDS             complement(3460..3951)
                     /gene="ilvN"
                     /locus_tag="VFF22_06035"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HZX16408.1"
                     /translation="MRHIISLLLENEPGALSRVVGLFSQRNYNIESLTVAPTEDPTLS
                     RLTLTTVGHDEVIEQITKNLNKLVEVVKLVDLSESAHVERELMLVKVKATGAQRAEVK
                     RTTDIFRGQIVDVTSSVYTIQLAGTSDKLDSFIQAIGTTSILETVRSGVTGIARGDKV
                     LSI"
     gene            complement(3954..5678)
                     /locus_tag="VFF22_06040"
     CDS             complement(3954..5678)
                     /locus_tag="VFF22_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HZX16409.1"
                     /translation="MELLSGAEMVVRFLRDEGVKNIYGYPGGALLHIYDALFKEPEVQ
                     HILVRHEQAATHMADGYARATGKAGVVLVTSGPGATNAITGIATAYMDSIPMVVLSGQ
                     VPSTMVGTDAFQETDMIGISRPIVKHSFMIKHASEIPEVLKKAFYLAQSGRPGPVVVD
                     IPKDMTNPAEKFEYVYPKKVKLRSYSPALRGHSGQIRKAAELLLTAKRPVIYSGGGVI
                     MGGAAAPLTELAQMLNLPVTNTLMGLGGYPGTDRQFLGMLGMHGSYTANLAMHHADVI
                     LAVGARFDDRVINGASKFCPNAKIIHVDIDPASISKTIKADIPIVGPVDSVMTEMVAI
                     LKEIGEQLNKDALASWWKQIEEWRGGRIMFPYDKGDGSIIKPQTVIETLSEVTGGDAF
                     VTSDVGQHQMFAAQYYRFNKPNRWINSGGLGTMGFGFPAAMGVKLSFPDDEVACVTGE
                     GSIQMNIQELSTCLQYGLPVKIVNLNNGALGMVRQWQDMNYGSRHSHSYMESLPDFVK
                     LAEAYGHVGMRITDLKDLKPKMEEAFAMKDRLVFLDIQVDTSEHVYPMQIKDGAMRDM
                     WLSKTERT"
     gene            6085..6528
                     /locus_tag="VFF22_06045"
     CDS             6085..6528
                     /locus_tag="VFF22_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HZX16410.1"
                     /translation="MRRMIITSSLLLAMSATAMASQVYKWVDAQGVTHFSAQPPQGQD
                     ATSINTATPPPKPAVAEEKKAAPTFESIADPEQAAIDEKVKQEVAAKEVERKKYCEDV
                     RTNLAQLQNNPRVRVDVDGEVRRLSEEERQSRISEAQKAIAENCK"
     gene            complement(6544..6873)
                     /locus_tag="VFF22_06050"
     CDS             complement(6544..6873)
                     /locus_tag="VFF22_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HZX16411.1"
                     /translation="MDARVPVVAEQLLLIERELRVQGWWDAQAPSAQALSSQQPFCVD
                     TLAFEQWLQWIFLPRMKQVLEAGAALPSVSGIQPMAEQVYGGQAKKARALIKLLGEFD
                     QLIVGAS"
     gene            complement(6873..7571)
                     /locus_tag="VFF22_06055"
     CDS             complement(6873..7571)
                     /locus_tag="VFF22_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011063491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZX16412.1"
                     /translation="MNKWLIPVVTVSLVLAGCSSVPRGSIPVVEGGTAVAGAQSGTPG
                     GYGTPAAQPQALPEDSGVVVMVPGGGGSSAPIQSFPAGNAPLDTGSTTQPWSATPPAT
                     TQGGYVAPAPSGIPSGGLAMDEQLDGPVLALLTTAQQQQGGGDLNGAASSLERAQRIA
                     PREPQVLYKLAEVRLAQGDAAQAEQFARRGLSYASGRPALQASLWNLIAQARERQGDA
                     AGAAQAREQARVSL"
     gene            complement(7729..10047)
                     /gene="mrcB"
                     /locus_tag="VFF22_06060"
     CDS             complement(7729..10047)
                     /gene="mrcB"
                     /locus_tag="VFF22_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HZX16413.1"
                     /translation="MTRIRSSRPRSKRRSGGMRPWLGWAIKLGLVGLVVLAVFAVYLD
                     AVVQEKFSGKRWTVPAKVYARPLELFVGQKLAKDDFLKELDALGYRRENAVAGPGGVS
                     VAGNTIELHSRGFQFYESTEPSQRVRVRFSGDYVAGLTQAGGGNLAVARLEPLLIGGL
                     YPAHQEDRVLIKLEQVPPYLVETLVAIEDREFFNHFGVSPKGIARAVWINATAGQLRQ
                     GGSTLTQQLVKNFFLTNERTLTRKATEAMMAVLLELHYEKKDILEAYLNEVFLGQDGQ
                     RAVHGFGLASQYFFSQPLSELKLDQVALLVGMVKGPTYYNPRRNPERALERRNLVLDV
                     LAAQGVANAEEVQAAKQKPLGITQRGTLADSSFPAFLDLVKRQLREDYQEQDLTEEGL
                     RIFTSFDPIMQLKAESALADTLKRLSGRKGVDEVEAGMVVTSPESGEVQAIIGSRQPR
                     FAGFNRALDAVRPIGSLIKPAIYLTALERPSQYTLTSLLEDKPFSIKGQDGQVWSPQN
                     YDRKAHGTIYLYQGLVNSYNLSTAKLGLEIGVPEVLKTLNRLGVEQNWPAYPSMLLGA
                     GGLSPISVANMYQTMANGGFNTPLRGIRAVLTAEGEPLKRYPYQIQQRFDSGAIYLLQ
                     NAMQRTMSEGTGRSVYSQLPRSLALAGKTGTSNDSRDSWFAGFSQDVLAVVWLGRDDN
                     GPTPLTGATGALQVWTDFMRKADPLPLDMPVPDNVVQAWVDASSGQGTDPSCPNAVQM
                     PYIRGSQPVAGPACGIQAPVNEVMDWVRGWLD"
     gene            10165..11727
                     /locus_tag="VFF22_06065"
     CDS             10165..11727
                     /locus_tag="VFF22_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003279886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZX16414.1"
                     /translation="MSQALIAALQNPALYPHPVEQFQVIETHISWVVLTGPFAYKLKK
                     PVNFGFLDFTELSAREHFCKEELRLNQRLTQDLYLEVLPISGSVDAPQLGGTGPAIEY
                     ALKMRQFPQSQLLSDVQARGELSEAHIDALAKQIADFHGSTPAVSASHALCTPEAIVA
                     PMRQNFEQIRPMLSDAADLQQLEALEAWTEANCARLEPLLAARAANGSIRECHGDIHL
                     GNATLLDDQVVLFDCIEFNEPFRLIDIASDAAFLAMDLEDRGLKAHAWRFVNGWLEHT
                     GDYAALELLNFYKAYRALVRAKVALFSLAHQTDAVQKAVTLRQYRNYANLAESYSAIP
                     SSFLVITHGVSAVGKSHVAMRLVEALGAIRLRSDVERKRLFGEQKDADLGKLSAGIYT
                     QDASAATYQRLHQLADAALHAGFPVVIDATYLKQAQREAASQVAEACGAPFLILECSA
                     PDEVIASWLEQRKAAGSDPSDATMDVVHAQQASREALTDDELLHSKRVDTHESASLDT
                     LIARIRQRLTGL"
     gene            11813..12157
                     /locus_tag="VFF22_06070"
     CDS             11813..12157
                     /locus_tag="VFF22_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010206561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HZX16415.1"
                     /translation="MSQPRQLDSPLYILIRNEQISAFNAQKPKDTVVDLSDGDFRGID
                     LRALNAEGINFTNAYFRGTDLRGLDLRNAQLEGASLAHAQISGAYFPVELTADEILMS
                     VNFGTRLRYNTR"
     gene            12154..12909
                     /locus_tag="VFF22_06075"
     CDS             12154..12909
                     /locus_tag="VFF22_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017245539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX16416.1"
                     /translation="MSGDHVSQLFGARADAYASFRPHYPQNLFAWLAAQCPERTRALD
                     IACGSGQASYPLLGHFKQVLACDASLEQLRAGAEQSGLQLFVAEAQTQPLPSHSLDLI
                     VVAQALHWFATPAFFAEVRRLLKPGGVFCAWCYSLMRIDGELDALIDDFYWNTLGGYW
                     PAGRASVDAGYRDIDNPFTPLTPPASAIESSWSLEQLLGYLRTWSAVQRYEQQNGIDP
                     LLQLQPKLRQAWDDAKQPRFVRWPLHFLAGTPN"
     gene            12949..13221
                     /locus_tag="VFF22_06080"
     CDS             12949..13221
                     /locus_tag="VFF22_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011063495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HZX16417.1"
                     /translation="MNDELQHLKNLGKTSAQWLHAVGIHSANDLRRLGAVSAYRAVRA
                     RGFRASKVLLYAIEGALLDVHWNELPPGHKAELNGQLDESSSHNKS"
     gene            13328..14128
                     /locus_tag="VFF22_06085"
     CDS             13328..14128
                     /locus_tag="VFF22_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZX16418.1"
                     /translation="MYLLGEQPAYADQLINRLQSIPTQLLDGLAPAGSPLQLERAEDL
                     AKILPGNQLFIIENGLLHAVVDERPLFYLQEGDLVGLRQGLDMPSCRYSSEEQLSLIP
                     YSRSEVFKHIYASEQRQELFIQYLIGHTALLSDALARLKQPEIRPSTGFQHFAAGEEL
                     IHQGDVADNVFIIIEGHAEAYVDGHKVGDVQKDEIFGAMAVFTREKRSATVLASEPCT
                     VMVIPKEQFLSLMQSNPRIAHSLIESMAKRIDLLNKEVTQLRLPAEAS"
     gene            complement(14405..14812)
                     /locus_tag="VFF22_06090"
     CDS             complement(14405..14812)
                     /locus_tag="VFF22_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZX16419.1"
                     /translation="MPAAAHALLTRYFNALNERDSRACLALLSADVLLDINQGPREQG
                     VQAFADYLERTQRCYRESIDNLQILTEPSGQRAACEFTLSGEYLITDDGLPDACGQSY
                     QLTAASFFEIRDGKICRVSQHFNLPEWLAQVDF"
     gene            complement(14867..15748)
                     /locus_tag="VFF22_06095"
     CDS             complement(14867..15748)
                     /locus_tag="VFF22_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HZX16420.1"
                     /translation="MRIALLLAAALLSACQASLPALPAWQSPDGLQHPELGQIVDLRT
                     GARLTPEQLLTRLAAAPKVLVGERHDNPDHHALQLWLLRALAAQRPQGSLLLEMLTPD
                     QQAKVEQVRAAIAAGQAPQDMLSALAWQPGWAWSLYGPLVQHVLRQPYPLLAANLERR
                     EVMQIYAQVPQLQGQASTAQLVREALFKQIRESHCNLLPESQLPAMLAVQQQRDRRMA
                     EALLAAPAPSLLFAGAFHVRRELGVPLHLQDLGAAEGVQVMILAEVGSQVVAESADYV
                     WYTPAQPEQDHCAKLRP"
     gene            complement(15759..16526)
                     /locus_tag="VFF22_06100"
     CDS             complement(15759..16526)
                     /locus_tag="VFF22_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HZX16421.1"
                     /translation="MLRAENLAVQRGPCTVLADINLELRPGEVLGVLGPNGAGKSTLL
                     GALCGELQPSHGDVWLDQRRLQDWPGSERAQRLAVLPQTSTLNFAFRVEEVVGMGRLP
                     HASGRVRDAQIIKQALEAADALHLAGRSYLALSGGERQRVHLARVLAQLWPGGEGQML
                     LLDEPTSMLDPLHQHTTLQATRAFVEQGAAVLVILHDLNLAARYCDRLLLLNGGRPHA
                     LGSPEEVLRAEPLQAVFGLEVLVQQHPERGHPLIVAR"
     gene            complement(16526..17563)
                     /locus_tag="VFF22_06105"
     CDS             complement(16526..17563)
                     /locus_tag="VFF22_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010562701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZX16422.1"
                     /translation="MNPVIRPRSLFIALSLLLALALWLSLALGPVSLPLLDTLKAALR
                     LLGLAQPGDDLAQAELILGQIRLPRTLLGLLVGAVLALCGVAMQGLFRNPLADPGLIG
                     VSSGAALGASIAIVGGAALGGLPEAFAPYLLSLFAFIGGLGVTALVYRLGRRDGETNV
                     ATMLLAGIALTALAGAAIGLFTYLADDATLRTLTFWNLGSLNGASYSRLWPLLLVTAA
                     VALWLPRRAKALNALLLGESEARHLGFAVERVKAELVFCTALGVGAAVAAAGMIGFIG
                     LVVPHLVRLLVGPDHRVLLPASALAGASLLLLADLVARLALAPAELPIGIVTALIGAP
                     FFLYLLLRGRT"
     gene            complement(17560..18459)
                     /locus_tag="VFF22_06110"
     CDS             complement(17560..18459)
                     /locus_tag="VFF22_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019817943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX16423.1"
                     /translation="MKRLNTLLALCASLLSCASVMAAEPLPQRWISSGGSLSEWVVAL
                     GGESRLVGVDSTSQHPQSLRALPSIGYQRQLAAEGMLTLRPDVLIGSEEMGPPPVLAQ
                     LRGAGVRVEMLSSKADLTSLQASLQRIGTLLGEPQRADQAFAAYQQRLQQQADWLVTA
                     QRQQAAPGVLLLLGHAGGSPMAGGKDTSADWLIERAGGRNLASHSGYKALSTEALLAL
                     DPEVVIIADRSLAGDAAREALLQQNPALASTRAARNQRLLMLDPTLLVGGLGPRLPDG
                     LAALSAAFYPASQPLVAEAKPTP"
     gene            complement(18446..18877)
                     /locus_tag="VFF22_06115"
     CDS             complement(18446..18877)
                     /locus_tag="VFF22_06115"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZX16424.1"
                     /translation="MIRLPAAGSSQGNLLPDLTPLLDVIFIVLVFFLLTAQTPLLELP
                     VQLPQSREALPTASAGSGERIQIQLSAEGSWRFNGVQQADFNTLRTELSQAFVADNTA
                     GLDLALDRQAPLSAFLDLMALLQQQGIQDSRILLEANHETP"
     gene            complement(18874..19515)
                     /locus_tag="VFF22_06120"
     CDS             complement(18874..19515)
                     /locus_tag="VFF22_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZX16425.1"
                     /translation="MSQWWSAMGPLGWPLALCSLLALALILERLSVFLRLQPLSTAAG
                     RAAVSACRTCEREHCKGRARGWRYGLALLLKHSALPAVQREELLGCWLLEERQRLNRQ
                     LRLLQLIGMLAPMLGLLGTVLGMLEMFANIAGQNSPVTPALLADGLWQALYTTVWGLL
                     IAIPALAAGQGFALWAERYLEGVQALLNRCQLALDGLELELPANQPAAQWVLP"
     gene            complement(19517..20587)
                     /locus_tag="VFF22_06125"
     CDS             complement(19517..20587)
                     /locus_tag="VFF22_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChuX/HutX family heme-like substrate-binding
                     protein"
                     /protein_id="HZX16426.1"
                     /translation="MSTHTQSVAPNNDLYLAWQVLRSEQPRLRARDAAERLAVSEAEL
                     AASRLDIDAVRLQPDWAQLLPALGELGYVMALTRNEHCVHERKGYYREVTVTGNGQMG
                     LVVSADIDLRLFLGGWASVFAIEEQTGKGTQRSIQVFDRQGTAVHKVFLTDDSQLSAW
                     APLLESFRMAEQSAELELLPLPQAVPVQADAQVDVNALRVGWSTLKDTHHFFALLKKH
                     GTTRTQALRLAGSEWAEPLAVRELPKLLEQAGAREVPIMVFVGNRHCIQIHSGPVRNL
                     RWMDTWFNVLDPEFNLHLQTTGVTELWRVRKPSTDGVITSWEAFDANGELVVQLFGAR
                     KPGIPELEDWRALAESAPALEC"
     gene            complement(20609..21313)
                     /locus_tag="VFF22_06130"
     CDS             complement(20609..21313)
                     /locus_tag="VFF22_06130"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZX16427.1"
                     /translation="MLRLSLFLLLSLALHAAGWLLHGEAQPPALSAAQTREAPQVLRL
                     AAVLQQVAPAPAKLPEQPQPQPQPQRQAVVPSKVPVAKPVAQAAVAKPKSAVVAQPAQ
                     SQPAPAQPSHTTRQSAAVVAAAQAAPAKSVAAKPATEVLSSKPSFREPPRQPSYPSQA
                     RRRNQQGVVLVEVRLDERGQQRSLNVLRSSGVDSLDRAALEAVAKWRFRPETTGGQAV
                     PSRVQIPIQFALTASR"
     gene            21444..23636
                     /locus_tag="VFF22_06135"
     CDS             21444..23636
                     /locus_tag="VFF22_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016562376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HZX16428.1"
                     /translation="MHRSPPFARRPWLALLLLSPSLALGAEKAPNQFDTVTVTATRTE
                     QTLDQVPSTVSVQTERQIDQQNIKNIKDLVRYEPGVSVGGSGDRFGLSGFTIRGIGGN
                     RVLTQVDGVGMPDAFSFGGFLNAKRDYVDLDTVKQVEIIRGPASSLYGSDAIGGAVSF
                     LTKDAGDYLDEGDDAYARLKTGYDGSDDSWQRSATFAARLGQVDGLLHLGRRSGQATD
                     THSGQGGIGATREQANPLDYRTDNLLSKFGWDYSDSGRLQLTYERYQDDTDTRVLSNY
                     SNTATIRTQDAEDRVDRERYSLAHTQQFDTLLVDQIHSQLTYQDSQTRQQTFEDRVLA
                     GQPRYRTRDSYYEEKLWAFNTKLDKAFSIGRTDHALVYGLDVKQLKNADLREGGETVL
                     ATGVTTPVLPTSDFPDPTTREYALFAQDSISIGRWTLLPGLRYDHYEMSPHVTNKYLN
                     SQASDANPSDFKDSALSPKLGVTYQLDDAHSVYGQYAAGFRAPQAIEIFGEFANPGMY
                     RTLANTDLKAETSDSFELGLRGQYAIGSFGAALFYNQYDDFIEQVSRPSSVPGFPFGE
                     FQYVNRDRVTIRGAEAKGELFLDQLGLPAGWKARGSVAYARGKDEGTGQPINSVDPLK
                     GVFGLGYSEPSGKFGGELSWTLVAAKTRVDMTQTANHEQTSGYGVLDLNGWWQMTDAF
                     AVNAGLFNLTDKEYWQWGDVRGLTENSPSIGRFSQPGRHAAVNLVWEI"
     gene            23763..24089
                     /locus_tag="VFF22_06140"
     CDS             23763..24089
                     /locus_tag="VFF22_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010895682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HZX16429.1"
                     /translation="MLRLCAPDEVAEGQSRVFEVAGEKLFVVRKDGQLYAYRNRCPHR
                     GIPLEWLPDQFLDHSASLIQCATHGALFLIESGECVAGPCAGQSLQELTIREDDQGIW
                     VELNEA"
     gene            complement(24173..25063)
                     /locus_tag="VFF22_06145"
     CDS             complement(24173..25063)
                     /locus_tag="VFF22_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
                     /protein_id="HZX16430.1"
                     /translation="MSTSSAPRYPLVLVPGLLGFVSLLGYPYWYGIVTALRRIGATVF
                     PVLVSSVHSTEVRGEQLLQRIADVLQQTGAEKVHLIGHSQGALTARYAAALKPEWVAS
                     VTSVAGPNHGSELADFLKRKAPHGSWRERLLTRLLRGVARCMAWLERGYRGEPLPMDV
                     AAAQQSLTSEGVAAFNAQYPQGLPEVWGGEGAAEVNGVRYYSWSGTIQPGITDKGGNR
                     FDGPNVACRMFAKTFQREVGQCDGMVGRYSSHLGTVIGDQYPLDHFDIINQTFGLVGK
                     GAEPVKLFVEHAKRLHAAGL"
     gene            25185..25922
                     /locus_tag="VFF22_06150"
     CDS             25185..25922
                     /locus_tag="VFF22_06150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX16431.1"
                     /translation="MTMHTIERIYPKDLNPLNPDDQASLQIHMQRYEFAAEHLSGQRV
                     LDMACGCGYGTALLAERNPDKQVTGVDIDPAAIAYAREHYQLANLRYECADAEQFSSD
                     QGFDCIVSLETIEHLPRPHVLLANYARLLAEGGKVIASVPITPTLDGNPHHLHDFSKR
                     SFYALFRQQGLHAQQHLEQIQWWQFKGLFSKQVDQQKQHRSEGVGNAVLLYYRKRPLY
                     LFSRLYSMLRYGFSNRYLTCVFTRQPA"
     gene            complement(25903..26991)
                     /locus_tag="VFF22_06155"
     CDS             complement(25903..26991)
                     /locus_tag="VFF22_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003116117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HZX16432.1"
                     /translation="MRGDRELSMDEMNAFGNELDALRQSTLADLGETDARYIRRIRAA
                     VRLCYWSGRGLLMLGWFPPTWLLGTLLLGLGKILENMELGHNVMHGQYDWMNDPEFAG
                     RSYEWDIAGPSDFWRHTHNHVHHTWTNVLGMDDDVGYGVVRLFPEQRWKPFYRWQPLW
                     VMIQALLFQYSVAIQHLRLDKVYKGKLSKAEVRPLIKQFNAKILRQWGKDYLFFPLLA
                     LLIGANALAVLAGNVVANLIRNLWTFTVIFCGHFTEQAAVFSKESVVGESRGHWYLRQ
                     LRGSSNLNGGPLFHILTGNLSHQIEHHLFPDLPARRYAALAVEVREIAERYGQVYNNG
                     SLWSQFSTVLRRIWIYRRRPAEAMPAGA"
     gene            complement(27009..28124)
                     /locus_tag="VFF22_06160"
     CDS             complement(27009..28124)
                     /locus_tag="VFF22_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HZX16433.1"
                     /translation="MALLPFAWVRGLASLLYPLRVLVAHGWLRESDVDAGLALLHPAL
                     RLNRAFARVTQRQWVAADMLELTLQANGNWRGAQPGQHLQLYLERDGVRLSRSYSLTA
                     VQADGSLQIAIKHQAGGRVSPYLLEHLAVGDVLELGPVYGELSWPEAQQGVLLLAAGS
                     GITPLLGMLRAALAQGFSAPITLLHYVREQGQRGFVAELQALQAQHGNLQVHWSLTAA
                     GADPEGLAGRFTAEHLAEVTQLDQRRVLACGPAGFVAQVQQWWQAARLPGALQVEAFT
                     APVLRADVSLRQVRLGFARSHQQTTANNQHSLLEQAEAHGLQPVHGCRQGICASCTCT
                     LLSGTVRDLRNGVLFSEPGQPIRLCVSAPHTDVEIDL"
     gene            28337..28927
                     /locus_tag="VFF22_06165"
     CDS             28337..28927
                     /locus_tag="VFF22_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005763069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZX16434.1"
                     /translation="MDAARSLMDSGRGFGSLSLREVARCAGIVPTGFYRHFEDMDALG
                     LALIAEVGETFRETIRQVRHHEFELGGVIDASVRIFLDAVAANRSQFLFLAREQYGGS
                     LPVRQAVGALRERITADLVADLAAMPKLQHFDSPAQNVIADLVVKTVFATLPELIDPP
                     AAPLPMHASPEVKMIEQLRFIFIGAKHWQGLGQTRD"
     gene            29105..29605
                     /gene="ureE"
                     /locus_tag="VFF22_06170"
     CDS             29105..29605
                     /gene="ureE"
                     /locus_tag="VFF22_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HZX16435.1"
                     /translation="MLVIHRRIAAQPCWDAELELSFEARSKSRLRCFSRSGEDVGLFL
                     ERGQPALHDGECLQAEDGRIVRVVARPEPLLHVTCTNPFELMRAAYHLGNRHVALQLG
                     DGWLRLPDDYVLKAMLEQLGATVELIDAPYQPEQGAYGGGHHHSHAGDAEFSYAPRLH
                     QFGARK"
     gene            29714..30388
                     /locus_tag="VFF22_06175"
     CDS             29714..30388
                     /locus_tag="VFF22_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011332213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HZX16436.1"
                     /translation="MNKAWALLRLASPQLPIGGYSYSQGLEMAVEQGLVDNAESAERW
                     IADQLLLNLARFEAPLLLAHCKAAASEDWAQLRHLAEQQRASRETRELRLESRQMGYS
                     LQQLLSGLPELDQPAQQLFSELAEPGLAVGWALAARAWQIEPEDALAAWLWGWLENQL
                     AVLMKTLPLGQQAAQRLTSKLLPLLDQAQRQASELQPQHWGSAAFGLALASMAHERQY
                     SRLFRS"
     gene            30408..31022
                     /gene="ureG"
                     /locus_tag="VFF22_06180"
     CDS             30408..31022
                     /gene="ureG"
                     /locus_tag="VFF22_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HZX16437.1"
                     /translation="MNSQPLRIGIGGPVGSGKTALTLALCLALRERYNLAVVTNDIYT
                     QEDAQFLVRNEALAPERIIGVETGGCPHTAIREDASINLEAVDQLNRRFPGLDLIIVE
                     SGGDNLSATFSPELSDLTIYVIDVSAGDKLPRKGGPGICKSDLLVINKVDLAPLVGAS
                     LEVMERDTLKMRGDKPFVFSNQKIGQGLEQIIAFIERHGMLTAA"
     gene            31122..31694
                     /locus_tag="VFF22_06185"
     CDS             31122..31694
                     /locus_tag="VFF22_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016985390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HZX16438.1"
                     /translation="MNLRKALFAIALFLSPAMAFAHAGHDHSGLLAGLAHPVFGLDHL
                     LAMLAVGLCAAQQAGASRWALPLTFVGSMLIGGLLGFNGLQIPLMETGIAASVLAFGL
                     LVAVAIRLPMVISIALTALFALTHGVAHGLELPELASPWGYAVGFVVATAALHASGYA
                     LVRLLPQAAAPVVRVLGAASAVTGAWLLAG"
     gene            31866..32777
                     /locus_tag="VFF22_06190"
     CDS             31866..32777
                     /locus_tag="VFF22_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HZX16439.1"
                     /translation="MLRVARFLSAPLLLLAIALGVLALTSKPAQPAAVLSSFAEQPLV
                     IAHRGGKGLWPENSLFAFQRASALGVDMLEMDLHLSSDGELVVIHDSTLERTTDGEGP
                     VAALSLTQLQALDAGYRWSADGGQSYPYRGQGIRIPRFAEVLDSFPDTPKVIEIKVPD
                     VGMEALLCDALEAYQQRDLVIVGSFYDRSLQLFREQCPGVATSAGPGSVRLLVALNWV
                     GLGSLLSPSYQALQIPEAHGGLPVASPSLLKTAAQRGLNVQLWTINEQPDMRRLLDQG
                     AHALITDYPDRALQVLGRSTQISALED"
     gene            33774..34715
                     /locus_tag="VFF22_06195"
     CDS             33774..34715
                     /locus_tag="VFF22_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010456870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HZX16440.1"
                     /translation="MAGASLLTLLDDIAAVLDDVAVMTKVAAKKTAGVLGDDLALNAQ
                     QVSGVRAERELPVIWAVAKGSFKNKLILVPAALLISAFAPWAVTPLLMFGGAFLCYEG
                     FEKLAHKLLHSKAEDQAQHAELLHAVADPEVDMLAFEKDKIKGAIRTDFILSAEIIAI
                     TLGTVALASFGKQVAVLSAIALVMTVGVYGLVAGIVKLDDAGLYLNQRGGEGLGGRLQ
                     RSFGRGILFTAPYMMKGLSILGTAAMFMVGGGILTHGVHAAQEWIDQLAQSVAASSAL
                     LAALLPTLLNAIAGIIAGAVALLLVSLATRAWKAIKG"
     gene            complement(34763..35662)
                     /locus_tag="VFF22_06200"
     CDS             complement(34763..35662)
                     /locus_tag="VFF22_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016704520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZX16441.1"
                     /translation="MSSACYQILHDTHYRYASPVSLAQQLAHLWPRACPWQRNHQQQL
                     LVSPQPCKRQDALDVFGNPLTRLVFERPHDELKVSARLQVEVLARPALDLQASPPWEE
                     ACTALRYSGQALLSGVLDATRFRFESPYVHLKQVFADYAADCFSPGRPLLAAVDGLMQ
                     KIFSEFSFDAAATQVATPLLQVLEERRGVCQDFAHLMLACLRSMGLSARYISGYLLTQ
                     PPPGQPRLIGADASHAWVSVFCPQQGWVDFDPTNNIRPALEHITLAWGRDFADVSPLR
                     GVILGGGSHDPEVQVTVLPLADR"
     gene            complement(35659..38151)
                     /locus_tag="VFF22_06205"
     CDS             complement(35659..38151)
                     /locus_tag="VFF22_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HZX16442.1"
                     /translation="MPDLLADYPLTSGAYHELFDADGKVRPHWRLLLERLQRSSPAQL
                     AQRQALLARQIQENGVTYNVYADPDGADRPWELDLLPNLIPAEEWQQIAAGVAQRAGL
                     LNAVLADLYGPQQLLADGLLPAELVFGHNNYLWPCQGVQPPGGTYLHLYAVDLARAPD
                     GCWWVTADRTQAPSGAGYALENRQILSRAFPELYRDLRVQYLAGFFRTLQDTLARQAP
                     AGSETPLVVLLTPGRFNESYFEHLYLARQLGYPLVEGSDLTVRDAKVYLKTLAGLRRV
                     HAVLRRLDDDFCDPLELRTDSALGVPGLLEAVRQGNVLVANALGSGVLESPGLPGFLP
                     AIAEKLLGEELLLPSIASWWCGEPPVLDKALEQLDELLVRASFPSQSFAPVFGRDLDE
                     AQRAELAERLKARPYAYVAQALAQLSQAPVWQPEEGQLAPRAIGMRVFAVASAEGYRV
                     MPGGLTRVAAEADAEVVSMQRGGASKDTWVLGERHAGGEPWQWLRPLGVADLVRSDPY
                     LPSRVVENLYWFGRYSERCEDGARLLRIMLARYVDDDDDPQALQTALSLAESLGLLPD
                     AESGELQQRLLEALLGEDWPDSLRGNLQRLQWVAGSVRGKLSQANWQALLELQREAQG
                     LDAEQADFGELLDFLDRLLLSLAALSGFALDDMTRDDGWRFLMIGRCIERLQFLCDSL
                     ANFLRSSAAQDQSALEWLLELGNSSITYRTRYLASAQLIPVLDLLLLDEQNPHAVLFQ
                     VRTLLRSLARLGERFELPSERRLKHLEGQLARFSLGSLENPLFGSTSVQEVLDGLAGL
                     LENISQASAEISDRLGLRFFVHVDASQRTQSS"
     gene            complement(38226..41516)
                     /locus_tag="VFF22_06210"
     CDS             complement(38226..41516)
                     /locus_tag="VFF22_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZX16443.1"
                     /translation="MSIHIALHHVSHYRYDRAVNLGPQVVRLRPAPHSRTRVLSYALK
                     VEPGKHFINWQQDPQGNYLARLVFPEKTRELKIEVDLVAEMAVFNPFDFFLEPYAEKI
                     PFAYTEGEQLELAPYLVKLPAGKLFSEYLASIERTPLPSVDFLVALNQRLATDISYLI
                     RLEPGVQTPEESLQKGSGSCRDSAWLLVQLFRHLGLAARFVSGYLIQLKADMKSLDGP
                     SGTEVDFTDLHAWCEVYLPGAGWIGLDPTSGLFAGEGHIPLACSPEPSSAAPISGGVD
                     ESECEFSHEMWVERIWEAPRVSKPYSDEQWQAIVALGQKIDEDLQAQDVRLTMGGEPT
                     FVALDYPDDAEWNTAALGPNKRRLATDLFHRLREHYASKALVHFGQGKWYPGEQLPRW
                     SLNAYWRRDGEPIWQNPALYADEQQDYGVDAAKAADFLTLLAERLGVGGKNCVPAYED
                     RFYYLWRERKLPSNVTAEDSRLADALERERLRKVFEQGLDKVVGHILPLARHAKQLGW
                     QSGSWFLRDEHCRLIPGDSAMGYRLPLDSQPWVSEGDYPYVNPEDPSQVFAPLPAAPQ
                     IKPQSRGVWSGSQSGVNKRPLLGQSAAGVVRTALCAEPRDGKLYLFMPPLVRLEDYLE
                     LVAAIEATAAELKCPVLLEGYEPPLDPRLQFFRVTPDPGVIEVNIHPAASWDELVERT
                     EFLYEQARECRLSSEKFMIDGRHTGTGGGNHFVLGGATPADSPFLRRPDLLRSLISYW
                     HNHPSLSYLFSGLFIGPTSQAPRIDEARNDALYELEIAFAQMPEVGRDCQPWLVDRLL
                     RNLLVDVTGNTHRSEFCIDKLYSPDSASGRLGLLELRAFEMPPHAQMSLAQQLLIRAL
                     IARFWREPYKPARLVRWGTELHDRYLLPHFIEQDFADVLQELDGCGYRLHSEWFAPHF
                     EFRFPKSGDFNVKGIELELRQALEPWHVLGEEGGGGGTVRYVDSSLERMQVKLSGLPS
                     DRYVLTCNGVPVPLRPTGKVGEFVGGVRFRAWQPANCLHPTIEVHAPLVFDLVDSWMS
                     RSLGGCQYHVAHPGGRNYDSLPVNAYEAESRRLARFFRLGHSPGKRPTLAPINNNEMP
                     MTLDLRLT"
     gene            complement(41686..42798)
                     /locus_tag="VFF22_06215"
     CDS             complement(41686..42798)
                     /locus_tag="VFF22_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SO2930 family diheme c-type cytochrome"
                     /protein_id="HZX16444.1"
                     /translation="MRRLLLGALLLLAGCEQQPAPLYLPQGDNYPEKLSEWGMLQRAD
                     GFIAQVPEALPYDLNTPLFTDYAHKLRTVWMPTGTAANYGEAHFDYPVGTVLSKTFYY
                     PKDGQGRLLLNEQDDRNPQQGLELARVQLIETRILLKQQDGWVALPYVWDEQQQEAHL
                     EWAGDSRQLSLLDAEGKTQAVAYQVPDANQCAGCHEETAGQGVNPLGPKARHLNKDFA
                     YADGSHNQLQHWQQLGRLQGVPAELQGVPQNALWPTQRSGESLEQQARSYLDANCSHC
                     HNPTGPGRTSGLLLDPATTLGISYGLCKQPVAAGKGSGDRLVDIHPGQPDKSVLLFRV
                     ESVDPSMMMPELGRSTVHTEGVEVLQRWVASLQGDC"
     gene            complement(42798..44036)
                     /locus_tag="VFF22_06220"
     CDS             complement(42798..44036)
                     /locus_tag="VFF22_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HZX16445.1"
                     /translation="MRLALLSVLVLFVLLAACSEEPAPVADLDFQKDLQTQLIKVKPG
                     SVIEIPAGTYQLDRSLSLKVSGVTIKGAGMDKTILSFKGQKSGAEGLLVDASDFTIED
                     IAFEDTLGDSLKVVGGKNIIIRRVRTEWTNGPATENGAYGIYPVQTENTLIDGAVAIG
                     ASDAGIYVGQSRNVVVRNSRAERNVAGIEIENTIDADVYDNVATGNTGGILVFNMPNL
                     PQAGRVTRVFRNKIEGNNHKNFGHKGTPVASVPAGSGVVINSNDDVEVFDNDIGDHKT
                     ANIIISSYFSTGYTDLSTAENFDPYPEGIHIHGNRFGPGGDSPDHLELKALKVSQFGL
                     NGRLPDILWDGYVNLDILVDGKLPADRAICINNGEAGMINVDGPNNYKNISTEMADYR
                     CSLPALPAVKLASADADQGA"
     gene            44201..44377
                     /locus_tag="VFF22_06225"
     CDS             44201..44377
                     /locus_tag="VFF22_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16446.1"
                     /translation="MYIYRLVLILVVGIYLFSPAIMDWWIDPNGAWYRPYLLWLILIV
                     VTFILQSQRDADEL"
     gene            44361..47315
                     /locus_tag="VFF22_06230"
     CDS             44361..47315
                     /locus_tag="VFF22_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX16447.1"
                     /translation="MLTSFSLSQLILISAAYLLILFGVAWISEHGLIPRWVIRHPLTY
                     TLSLGVYASAWAFYGTVGLAYQYGYGFLASYLGVSGAFLLAPVLLYPILRITRTYQLS
                     SLADLFAFRFRSTWAGALTTVFMLIGVLPLLALQIQAVADSIGILTREPVQERVALAY
                     CGLIILFTILFGARHIATREKHEGLVFAIAFESVVKLVAISAIGLYALYGVFDGPQDL
                     QSWLVQNQSSLTSLHTPLQEGPWRTLLLVFFASAIVMPHMYHMTFTENLNPRAMVSAS
                     WGLPLFLLLMSLAVPLILWAGLKLGATTNPEYFTLGVGIAVNSQTLALIAYVGGLSAS
                     SGLIIVSTLALSGMVLNHVVLPLYQPPSEGNIYRWLKWTRRALILLVIMAGYGFYLLL
                     GAEQDLANLGIVAFVATLQFLPGVLSVLYWPTANRKGFIAGLMAGIGMWVVSMLLPML
                     GNLQGLYLPWFNVIYVLDDSSWHLAAIGSLAANVLVFTLVSLFTEASPEEKSAAEACA
                     VDNVRRPQRRELLAASPQEFAAQLAKPLGAKTAQHEVEQALRDLHLPFDEGRPYALRR
                     LRDRIEANLSGLMGPSVAQDIVETFLPYKAGSETYVTEDINFIENRLEDYHSRLTGLA
                     AELDTLRRYHRQTLQELPMGVCSLAKDQEILMWNKAMEELTEIPAQRVVGSRLNTLGE
                     PWRGLLQNFIELPDEHLHKQRLALDGQTRWLNLHKAAIAEPLAPGNSGLVLLVEDLTE
                     TQMLEDKLVHSERLASIGRLAAGVAHEIGNPITGIACLAQNLREEREGDSELTEISEQ
                     IIEQTKRVSRIVQSLMSFAHSGSHQNSEEPVCLAQVAQDAIALLALNRRSVEVQFFNL
                     CDSDHWVVGDPQRLAQVLINLLSNARDASPPGSAIRVKSERFEHTIDLIVEDEGSGIP
                     KAIIDRLFEPFFTTKDPGKGTGLGLALVYSIVEEHYGQITIDSPADPELQRGTRIRVS
                     LPRHVEATSIAT"
     gene            47392..48819
                     /locus_tag="VFF22_06235"
     CDS             47392..48819
                     /locus_tag="VFF22_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZX16448.1"
                     /translation="MPHILIVEDETIIRSALRRLLERNQYEVSEAGSVQEAQERFSIP
                     SFDLIVSDLRLPGAPGTELIKLGQGTPVLIMTSYASLRSAVDSMKMGAVDYIAKPFDH
                     DEMLQAVARILRDHQQAKSAPVPAASTAKVSSDKVVDNSNGEIGIIGSCAAMQEMYSK
                     IRKVAPTDSNVLIQGESGTGKELVARALHNLSRRAKAPLISVNCAAIPESLIESELFG
                     HEKGAFTGASAGRAGLVEAADGGTLFLDEIGELPLEAQARLLRVLQEGEIRRVGSVQS
                     QKVDVRLIAATHRDLKSLSKIGQFREDLYYRLHVIALKLPPLRERGTDVIDIARAFLI
                     RQSTRQGRLDLSFAADAEQAIRHYPWPGNVRELENAIERSVILCEGSSISAELLGIDI
                     ELDDLEDDFSGLPAQSGGLGHEPSEPTEDLSLEDYFQHFVLEHQDHMTETELARKLGI
                     SRKCLWERRQRLGIPRRKSSGATAG"
     gene            49520..50920
                     /locus_tag="VFF22_06240"
     CDS             49520..50920
                     /locus_tag="VFF22_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019582861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HZX16449.1"
                     /translation="MLKKLFKSFRTPIRRGNHSHSTPEVLSSSQHPIERGEISRYAIS
                     VVERLQQAGYQAYLVGGCVRDLLLDIDPKDFDVATSATPEQVRAEFRNARVIGRRFKL
                     VHVHFGREIIEVATFRANHPEGDEEENSNLSSRNESGRILRDNVYGSLEDDAQRRDFT
                     INALYYDPSTERILDYANGVHDIRNHLIRLIGDPTQRYQEDPVRMLRAVRFAAKLDFD
                     IEKHSAAPIYKLAPLLGGIPAARLFDEVLKLFLAGYAVYTYELLVDYGLFGQLFPASA
                     EALKLNPDYTDQLIRNALDSTDLRIQQGKTVTPAFLLAALLWPALPARVIKLQDRGMP
                     PIPAMQEAAHELISEQCLRIAVPKRFTLPIREIWDMQERLPRRSGKRADLLLDNLRFR
                     AGYDFLLLRESAGEQTDGLGEWWTDYQDANDSERRDMIRDLSGKDDGTGGPRKRRRSG
                     GAKRKRSADASSTTGE"
     gene            50924..>51225
                     /gene="folK"
                     /locus_tag="VFF22_06245"
     CDS             50924..>51225
                     /gene="folK"
                     /locus_tag="VFF22_06245"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007990944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HZX16450.1"
                     /translation="MERIYIGMGSNLVDPAEQLRSATLALAQLPQSELVGVSAFYQSD
                     SLLPGQPRYTNAVAALDSTLAPLELLDALQSIENDQGRERLERWGPRTLDLDILLFG"
BASE COUNT        10585 a        16576 c        14771 g         9293 t
ORIGIN      
        1 gaggcgaatt ctacagcgtt tcaaaccgct gtcaaccgcc tcttttaccg ctttcgatca
       61 acttgaccga accccttcag acttaaacac taccccctga agaggaggcg cattctacgc
      121 agctttcgct gctttgcaac ccccttttta aatataactt cttgttttat aagaagttta
      181 aggagccact tacgcctgaa gaggtgcgca ttatagggac taccaaacga aggtcaatcg
      241 attgttatgc ttttattgca atacccacga actcaagggc tgtagccaca caatccacca
      301 gcgcgtcgcc tgcagaaacg acaaagccgt tgaagctgac gcttccaacg gctttgtcgt
      361 aactatcgcg cagcagctaa gcgctttttc cgcgacgcag gcgcagcagg tactgaatcg
      421 ggcccgaggc cgcgtatgcc aggaagatga tcagcagaat ccgcggcgga tcactgaaca
      481 ccacagcaaa caccagcacc accgccagga tagccacaaa aggcacgcgc cctttcaggt
      541 caagatcctt aaagctgttg tacttgatat tgctgaccat cagcatgccg gcagcagcca
      601 cgaggattgc aaccacaaag gccatattcg agcctttgat accgaaatca ctgaaggccc
      661 acaccgtacc cgccaccaca cccgcagcag ccgggctggc caagccgata aaatagcgct
      721 tatcaacact gccgatctgc gtattgaaac gcgccaaacg cagcgcagcc ccggctacat
      781 agatgaaggc gaccatccag ccgaccttgc ccatgctgcc caacgcccac tcaaacgcga
      841 gcaaggcagg cgccacacca aaagcaacca tatcggatag cgaatcgtac tcggcaccaa
      901 aggcgctctg ggtattggtc aagcgcgcga cacgcccatc cagcccatcc agcaccatgg
      961 caacaaacac cgcggcggca gctacataga agttgccgtt catggcattg atgatggcgt
     1021 agaacccggc aaacaggttg gccgtggtaa acagattggg cagcagatat ataccgcgat
     1081 gacggacctt ccgaccttcg gcgtcatgcc cctcttctat atgctcatca attggcagca
     1141 aactttcggc gtcggcggga gactgcggct catctggacg ttcattcatg aacaacacct
     1201 tgcaacggat ttggagaatt tcgacaggcg ctaacgaata gggttcacgc caacgcccat
     1261 gcaacaccca ggctttatac cagaagcctt gccgcactat gaaaaaacgc ggccttggcc
     1321 gcgttttttt cttatgtcat aaaaccttag ttcttggctt tatcgacgat tttgttggca
     1381 ccgatccacg gcatcatgga gcgcagttgc tcaccgatga tttcgatgcc gtgagcagcg
     1441 ttgttacgac gcttggcggt catcgaaggg tagccggttg cgccttcgct gatgaacatc
     1501 ttggcgtatt cgccgtcctg gatgcgcttc agagcattgc gcatggcttg acgggattcg
     1561 gcgttgatca cttccggacc ggtcacgtac tcgccgtact cggcgttgtt ggagatcgaa
     1621 tagttcatgt tggcgatacc gccttcgtac atgaggtcaa cgatcagctt cagttcgtgc
     1681 aggcactcga agtaggccat ttctggcgcg tagcccgcct caaccagggt ttcgaaaccg
     1741 gctttgacca gctcaacggt accgccacac agaacggctt gctcgccgaa cagatcggtt
     1801 tcagtctcgt ccttgaaggt ggtttcgatg atgccggtac gaccgccacc aacgccagcg
     1861 gcgtaggaca aagcaacatt cttggcgttg cccgaagcgt cctggtagat cgcgatcagg
     1921 tcaggaatgc cgccgccttt gacgaactcg gaacgcacgg tgtggcccgg tgccttcggc
     1981 gcgatcatga tcacgtcgag atcgctacgc ggcacaacct ggttgtagtg gatcgcgaag
     2041 ccgtgagaga aggccaaagt ggcgcctttc ttgatgttcg gctcgatttc attcttgtac
     2101 agcgcggatt ggaactcgtc cggggtcagg atcatgacta ggtcggcagc agcaacggcg
     2161 gaagcaacat cagtcacttt caggccgtgg gcttcagcct tggcaacggt ggccgagcct
     2221 ttgcgcaggc caacagtcac gtcaacgccg gagtctttca ggttgcacgc ttgagcgtgg
     2281 ccctgggaac cgtaaccgat gatggcaact ttcttaccct ggatgatcga gaggtcacaa
     2341 tctttgtcgt aataaacttt catgttcttg gctcttttaa tcgaaaaggg atggggtgca
     2401 tgcaccgcga cgaagacacc ttaagtgatc cgcgctattg cgtaaaatga tatatttgca
     2461 acacaacatg ccgatatttg aaacgattat ggatagtcac gcactcaggc tctttctctc
     2521 tctggccgac aacctgcact tcggcaaaac cagccgtgag cagcacgtca gcccttccgc
     2581 actcagccgc agcatcaagc aactcgagga cgaactgggc gcaccgctgt ttctgcgcga
     2641 caaccgctca gtccgcctga ctcgcgaggg acaacagttc cgcgagtatg cgagtgaggt
     2701 catgaacggc tggcaggcca tccgccagac cttcaagcag gaccagttga tcctgcacgg
     2761 cgagctctcc ctatattgct cggtaacggc cagctacagc ttcctttatg acattttgag
     2821 cagctttcgc cacgactacc cgcgcattga aatgaaactg cacaccggcg atgcagccaa
     2881 agccgtagag cgcgtgcaag aaggactgga agacctggcc atcggagccc gccccgacag
     2941 cttaccggcc ggcatcgagt tccaaccaat tgcccgctcc aagctgtgct ttatcggccc
     3001 acagtcacct cagctattga ccgaagaaca actgaaacac cccagcgcag ccagttggcg
     3061 ggacgtaccg atgatccttt cggaagaaga tctagcccgc acccgaaccg acctctggct
     3121 gaaaaaccac aacatcaaac cgctgattta tgcacaagtg agcggtaacg aagccatcgt
     3181 cagcatggtg agcctgggct ttggcattgg cgtggtgccg caaatcgtac tagacaacag
     3241 cccgttggcc gcacgcatcc gaatctacga tattcagcca ccgttaaccg gttacgaaat
     3301 tggcctgttc gcgctggaga aacgccttaa agatccgctg ataaacgcct tctggaatcg
     3361 ccaacgctaa acgctggaaa cgaggcattt atcccaaaaa gcaaaaagcc ctcgggagga
     3421 attcctccga gggcttttcg ttttaacagt gcaacgcaat cagatgctca gcaccttatc
     3481 gccacgcgca ataccggtca caccactgcg tacagtttcc agaatcgacg tagtgccgat
     3541 ggcctggata aagctgtcca gtttgtcact cgtgccagcc agctggatgg tgtaaacact
     3601 gctggtcaca tcgacgatct gcccacggaa gatgtcagta gtacgcttga cctcagcacg
     3661 ctgagcacca gtcgccttca ccttcaccag catcagctcg cgctcgacgt gagcgctttc
     3721 cgacagatcg accaacttga ccacctcaac cagcttgttg aggtttttgg tgatctgctc
     3781 gatcacttca tcgtgaccca cagtggtcag cgtcagacgc gacagggtcg ggtcttcggt
     3841 tggcgccaca gtcaggcttt cgatgttgta gttgcgttgc gagaacagac caaccacgcg
     3901 ggacagtgcg ccgggctcgt tttccagcag cagggaaata atgtgtcgca tgattaggta
     3961 cgctccgtct tgctcagcca catatcgcgc attgcaccgt ctttgatctg catcgggtag
     4021 acgtgctcac ttgtatcgac ctggatgtcg aggaacacca gccgatcttt catcgcaaag
     4081 gcttcctcca tcttcggttt tagatccttc agatcagtga tgcgcatgcc gacatggcca
     4141 taggcctcgg ccagcttgac gaagtccggc aacgattcca tataggaatg cgagtgacgg
     4201 ctaccgtagt tcatgtcctg ccactggcgc accataccca acgcaccgtt gttcaggttc
     4261 acgatcttca ccggcaaacc gtactgcaga catgtcgaca gctcctgaat gttcatctgg
     4321 atgctgcctt caccggttac gcatgcgact tcgtcatccg ggaagctcag tttgacgccc
     4381 atagctgcag ggaaaccaaa gcccatggtg cccagaccgc cggagttgat ccaacggttt
     4441 ggcttattga agcggtagta ttgagcagca aacatctggt gctgaccaac atcggaggtc
     4501 acgaacgcgt cgccaccggt gacttcggac agcgtctcga tcacggtctg cggcttgatg
     4561 atgctgccgt cgcccttatc gtaagggaac atgatgcgac caccgcgcca ctcttcgatc
     4621 tgcttccacc agctcgccag cgcatccttg ttcagctgct cgccgatttc tttaagaatc
     4681 gcgaccatct cggtcatcac actatcaacc gggccgacga ttgggatatc ggccttgatg
     4741 gtcttggaga tggaggccgg atcgatatcg acgtgaatga tcttggcgtt cgggcagaat
     4801 ttgctcgcgc cgttgatcac ccggtcatcg aagcgtgcgc caaccgccag gatcacatcg
     4861 gcgtggtgca tagccaggtt ggcggtgtag ctgccgtgca taccgagcat gccgaggaac
     4921 tgacgatcag tgcctggata gccgcccaaa cccatcaggg tattggtgac tggcagattg
     4981 agcatttgcg cgagttcagt cagcggcgct gcagcaccgc ccatgatcac gccaccaccg
     5041 gaatagatta ctgggcgctt ggccgtcagc agcagttcag ccgccttacg gatctgcccg
     5101 gagtgaccgc gcagagccgg gctataggag cgcagcttga ccttcttcgg atagacgtac
     5161 tcgaacttct cagccgggtt ggtcatgtcc ttcgggatat ccacaacaac ggggcctgga
     5221 cggccggatt gcgccagata gaaggctttc ttcagaactt ccgggatttc cgaggcgtgc
     5281 ttgatcataa agctgtgctt cacgatcggc cgcgagatac cgatcatgtc ggtttcctgg
     5341 aaggcatcag tgccgaccat ggtgcttggc acctggccag acaacaccac catcggtatg
     5401 gagtccatgt aggcggtggc aataccagta atagcattgg ttgcgccagg gccggacgtt
     5461 accagcacca caccggcttt accggtggca cgggcatagc cgtcagccat atgggttgcc
     5521 gcttgctcat gacgaaccag gatgtgctgg acttccggct ctttgaacag ggcatcgtag
     5581 atgtgtagaa gagcaccgcc cgggtaccca tagatgttct taacgccttc gtcacgcaaa
     5641 aagcggacga ccatttcagc gccggataaa agctccacgt tgttcacctc taaaacgcca
     5701 gaaaaccgtc cataggagga cggcctaaga taggtttact gccaagcaga gcatgagcga
     5761 cggtggtcgc cgactacgtc agctacaact gagcaagtat tgggaatacc ccaaagtgtt
     5821 gcggggtctt cccacccagc gcgaggtaac gcgttgcggg tgtaacaggt cggcgcgggt
     5881 gtgcacctca tgtctactga gctaaagcct gcttgcggcg gactccacta cagcgagcgt
     5941 ggaattgttg agattcggcg cggccaagtc aagcaatcag tgattgaatc tgcaatcttc
     6001 tgctgtgacg cagcgcagga tgacgattcg aacattttgg ttatagtagt cagcaggcac
     6061 cgcagctcaa tataaggaaa ccgcatgcgc cgcatgatca tcacaagcag cttgctgctc
     6121 gccatgagcg ccaccgccat ggccagccag gtttacaagt gggtcgacgc ccaaggcgta
     6181 acgcacttca gcgcacagcc accacaaggc caagacgcca ccagcatcaa cacagcaaca
     6241 ccgccgccaa aacctgccgt cgcagaagag aagaaagctg cgcctacctt cgaaagcatc
     6301 gcagaccccg agcaggcggc cattgatgaa aaggtcaagc aggaggttgc cgccaaagaa
     6361 gttgagcgca agaagtattg cgaagacgta cgcaccaacc tcgcacaact gcagaacaac
     6421 ccacgcgtac gcgtcgatgt agacggtgaa gtgcgtcgcc taagcgaaga agagcgccag
     6481 agccgtatca gcgaagcgca gaaggcgatt gccgaaaact gcaaataagc cgattggcgc
     6541 gattcagctc gcgccaacaa tcagctgatc gaattcaccc agcagtttga tcagcgccct
     6601 ggcttttttg gcctgaccgc catacacctg ctcggccatc ggctgaatcc cggaaacact
     6661 gggcaaggcc gccccagcct ccagcacctg cttcatcctc ggtaaaaata tccactgcag
     6721 ccactgctcg aacgccagcg tatcgacaca gaacggctgc tgactggata gcgcctgcgc
     6781 actaggcgcc tgcgcatccc accaaccctg cacgcgcagc tctcgctcga tcagcagcag
     6841 ctgctccgcc accacaggta cgcgcgcatc catcagaggc tgactcgcgc ctgctcacgg
     6901 gcctgggcgg caccagcagc atcaccctgg cgctcgcgcg cctgagcaat caggttccac
     6961 aggctggcct gcaaggccgg gcggccgctg gcgtaactta agccgcgccg ggcgaactgt
     7021 tcagcctggg ccgcatcgcc ttgagccaat cgtacttcgg caagcttata gagcacctgt
     7081 ggttcacgcg gggcaatacg ctgggcgcgc tccaggctgg aggcagcgcc attcagatcg
     7141 ccaccgccct gctgctgctg agccgtagtc aacaaggcca ggactggacc atccagttgc
     7201 tcatccatgg ccaaaccgcc acttggaata ccgctgggag ccggcgcgac atagccgcct
     7261 tgggttgtgg ctggcggcgt agcactccac ggctgcgtag tgctgccggt atccagcggc
     7321 gcattgcctg cagggaacga ttgaatcggc gcggaactac cgccaccacc cggcaccatc
     7381 accactacgc cggagtcttc cggcagggcc tgaggctgcg cagcaggtgt accgtaccca
     7441 cctggcgtac ccgattgcgc gccagcgacg gcagtaccgc cttcaactac cggaatcgag
     7501 ccgcgcggca cactgctgca acccgccagt accagcgaaa ccgttacaac tggaatcaac
     7561 catttattca catcacaccc tctcacttaa aacctgctca tgatctgctg cgcgtcagcc
     7621 ctgctgcgtt gaaaacagcc tcgaaatgct catgtacaaa agtacactca gcttcctcgc
     7681 ctgttttcgc cttgcaaggc tctagctcgc gagatcgtga acaggttttt aatccaacca
     7741 gccgcgcacc caatccatca cttcgttgac cggtgcctga ataccacagg ccggcccggc
     7801 aacaggttgg ctgccgcgaa tatacggcat ctgcactgca ttcggacaac tcgggtcagt
     7861 accctgaccg ctgctggcat ccacccaggc ctgcaccacg ttatccggca cgggcatatc
     7921 cagcggcagc ggatcggcct tacgcataaa gtccgtccac acctgcaatg cgccagtcgc
     7981 gcccgttagc ggggttgggc cgttgtcatc acggcccagc cacaccaccg ccagaacatc
     8041 ctgactgaag ccggcaaacc agctgtcgcg cgagtcgtta ctggtaccgg tcttaccggc
     8101 caaggccagg gagcgcggta actggctgta gaccgaacgt cctgtgcctt cagacatcgt
     8161 gcgctgcatg gcgttctgca gcaggtagat agcgccgctg tcgaagcgct gctgaatctg
     8221 atacggatag cgcttgagcg gctcaccctc agccgttaat accgcacgaa taccgcgtaa
     8281 cggcgtattg aagccgccat tggccatggt ctggtacata ttggccacgc ttatcgggct
     8341 cagcccacca gcacccagca acatcgacgg atacgccggc cagttctgct caacacccaa
     8401 tcgattcagc gttttgagca cctcaggtac gccgatttcc agaccgagct tggcagtcga
     8461 caggttgtag gagttgacca agccctggta caggtaaatc gtgccatgag ccttgcgatc
     8521 atagttctgc gggctccaca cctgaccgtc ctggcccttg atggaaaatg gcttgtcttc
     8581 gagcaggctg gtcaaggtgt actggctcgg gcgctccaac gcggtcagat agatcgccgg
     8641 cttgatcagc gagccaatcg gccgtaccgc atccagcgcc cggttgaaac cggcaaagcg
     8701 cggctgacga ctgccaataa tggcctgcac ctcaccactt tccggactgg tcaccaccat
     8761 cccggcttcg acttcatcca cccccttgcg cccggatagc cgcttcaggg tgtcggccaa
     8821 ggcactttcc gccttgagct gcatgatcgg atcaaagctg gtgaagatgc gcaggccttc
     8881 ttcagtcaga tcctgctcct gatagtcctc gcgcaactgg cgtttgacca gatcaaggaa
     8941 ggctgggaac gagctgtccg ccaaggttcc gcgctgggta atacccaagg gcttctgctt
     9001 cgcagcttgc acttcttcag cgttggcaac gccctgcgcg gccagtacat cgagcaccag
     9061 gttgcgccgt tccagcgcac gctcggggtt gcgccgcgga ttgtagtaag tcggcccttt
     9121 gaccatcccc accagcagcg ccacctgatc aagtttcagc tcggacagtg gttgactgaa
     9181 gaaatactgg ctggccagcc caaagccgtg caccgcacgc tggccgtcct gaccgaggaa
     9241 cacttcattg aggtacgcct caagaatgtc cttcttctcg taatgcagct ccagcagcac
     9301 cgccatcatg gcttcggttg ctttacgcgt cagggtgcgc tcgttggtca ggaagaagtt
     9361 cttcaccaac tgctgagtca gggtactgcc gccctggcgc agctgaccgg cggtggcgtt
     9421 gatccacacc gcgcgggcaa tgcccttggg cgacacgccg aagtgattga agaactcgcg
     9481 atcctcaatc gccaccaggg tttccaccag ataaggcggc acctgctcca acttgatcag
     9541 cacgcgatct tcctggtgcg ccgggtacaa gccgccaatc agcaacggct ccagccgcgc
     9601 caccgccaga ttgccaccac cggcctgagt caggcccgct acatagtcac cggagaagcg
     9661 cacgcgcacg cgctgcgacg gctcggtgct ttcatagaat tgaaaaccgc gtgaatgcag
     9721 ctcgatagtg ttgcctgcca ccgaaacacc accaggaccg gccactgcgt tttcgcggcg
     9781 atagcccagg gcatccagct ccttgaggaa gtcatccttg gccaatttct gcccaacgaa
     9841 cagctccagc ggtctggcat aaaccttggc gggcacagtc cagcgcttgc cggagaactt
     9901 ctcctgcacc acagcatcga gataaaccgc aaacacggcg agcaccacca aaccaaccag
     9961 gccgagtttg atagcccagc ccaaccaggg gcgcataccg ccggaacggc gtttagaacg
    10021 gggacgagaa gatcggatac gagtcatggc gcggcattat acgcactttg cccacttgcc
    10081 agcaggcctg ctcagcggtt tgcagactgg cgttgagcgg ccataatggc cgcctcttat
    10141 tcagtacaac gccaaggatc gcccgtgagc caagctctga tcgccgcact gcagaacccg
    10201 gccctctacc cccatcccgt agagcaattc caggttatcg aaacccatat ttcctgggtg
    10261 gtgctcaccg gcccctttgc ctacaagctc aagaagccgg tgaatttcgg ctttctcgac
    10321 ttcaccgagc tgagcgcacg cgaacacttc tgcaaggaag agctgcgcct caatcaacgc
    10381 ctgactcaag acctctacct tgaagtactg ccgatcagcg gcagcgtcga tgccccgcaa
    10441 ctcggcggca ctggtccagc catcgaatac gcgctgaaaa tgcgtcagtt cccgcaaagc
    10501 cagttgctca gtgacgtgca ggcgcgcggg gaactgagtg aagcgcatat cgacgccctg
    10561 gccaaacaga ttgccgactt ccacggctcg accccagcgg tctcggccag ccatgcgctg
    10621 tgcacgcccg aagcaattgt cgcgccgatg cggcagaact tcgaacagat ccgcccaatg
    10681 ctcagtgacg ctgccgacct gcagcagttg gaagccctgg aggcctggac cgaagccaac
    10741 tgcgctcgcc ttgaaccgtt gctggccgca cgcgcggcca atggctcgat ccgcgaatgc
    10801 cacggcgaca tccacctggg caacgccacc ttgctggacg accaggtggt gctgttcgat
    10861 tgcatcgagt tcaacgagcc attccgcctg atcgatatcg cctcggacgc cgctttcctc
    10921 gccatggacc tggaagatcg cggcctcaag gcccacgcct ggcgcttcgt caatggctgg
    10981 ctggaacaca ccggtgatta cgccgctctg gagctgctta atttctacaa agcctatcgc
    11041 gcgctggtac gcgccaaggt tgcgttgttc agcctggccc atcagaccga cgcagtgcaa
    11101 aaggcggtaa ccctgcgcca ataccgcaac tacgccaacc tggcggaaag ctacagcgcc
    11161 atcccgtcca gcttcctggt cattacccac ggcgtttccg cagtgggcaa aagccatgtg
    11221 gccatgcgcc tggtcgaggc actcggggcg attcgtctgc gctcggatgt agaacgcaag
    11281 cgcctgttcg gcgagcagaa ggatgccgac ctcggcaaac tcagcgccgg catctacacc
    11341 caggacgcca gcgccgccac gtaccagcgc ctgcaccagt tggccgacgc cgcactgcac
    11401 gccggttttc cggtagtcat cgacgccacc tacctcaagc aggcgcagcg cgaagcggcc
    11461 agccaggtag ccgaagcctg cggcgcgccc ttcctgattc tggaatgcag cgcgccggac
    11521 gaagtgatcg ccagctggct ggagcaacgc aaggccgccg gcagcgaccc atccgatgcc
    11581 accatggacg tcgtgcacgc ccagcaagcc agccgcgaag cgctgaccga cgatgagctg
    11641 ctgcacagca agcgcgtaga cacccacgaa agcgccagcc tggacaccct gattgcgcgc
    11701 attcgccagc gtcttaccgg cctgtaataa gcaaaatggg taatagagtt gcggcgcgag
    11761 cagagcgcgc cgcggcttct atactggcgg tgagtccata tggagatgcc ccatgagtca
    11821 gccccgacaa ctggatagcc cgctgtatat cctgatccgc aacgaacaga tttccgcctt
    11881 caatgcgcaa aagcccaaag acacagtcgt cgacctgagc gacggtgatt ttcgcggcat
    11941 tgacctgcgc gccctgaatg ccgagggcat caacttcacc aatgcctact ttcgcggcac
    12001 cgacctacgc ggccttgacc tgcgcaatgc ccagctcgaa ggtgccagcc tggcccacgc
    12061 acagatttcc ggggcctact tcccagtcga gctgacggct gacgagattc ttatgtcagt
    12121 gaatttcggc acccgcctac gctacaacac ccgttgagtg gcgaccacgt cagccagcta
    12181 ttcggcgccc gagcggacgc ctacgccagc tttcgtccgc attacccgca gaatctgttt
    12241 gcctggctag ccgcgcaatg ccccgagcgc actcgcgcgc tggatattgc ctgtggtagt
    12301 ggccaggcca gctatccgct gcttgggcac ttcaaacagg tgctggcctg cgacgccagc
    12361 ctggagcaat tgcgagctgg cgcggagcaa tctggtctgc agttgttcgt cgccgaggcg
    12421 cagacgcagc ccctgcctag ccacagcctg gacctgattg tggtggcgca ggcgctgcac
    12481 tggtttgcca caccggcgtt ctttgcagag gtgcgccgcc tgcttaagcc aggcggggtg
    12541 ttctgcgcct ggtgttacag cctgatgcgc atcgatggtg agctggacgc gctgatcgac
    12601 gatttttact ggaataccct cggcggctat tggccagcag gccgcgccag tgtcgatgcc
    12661 ggctaccgcg atatcgacaa cccctttacc ccacttaccc cgcccgcctc ggcgatcgaa
    12721 agcagctggt cgctggaaca gctgttgggc tacctgcgca catggtccgc agtgcagcgc
    12781 tacgaacagc aaaacggcat cgatccactg ctgcaattgc agccaaagct gcgccaggcc
    12841 tgggatgatg ccaaacagcc acgttttgtc cgctggccgc tacactttct ggcaggcacg
    12901 ccaaactgac tccacacgca cgactgccca gcagcgcaag gaggcttgat gaacgatgaa
    12961 ctgcaacacc tgaagaacct cggcaaaacc tcggcccagt ggttacacgc agtcggcatt
    13021 cacagcgcca atgatctgcg tcgattaggt gcggtaagcg cttatcgagc ggtacgcgcg
    13081 cggggcttca gggcatccaa ggtgctgttg tatgcaattg aaggcgcact gctggacgtg
    13141 cactggaacg aactgccacc cggccataaa gccgaactga atggccagct cgatgaaagt
    13201 tcgtcgcaca acaagagcta gctcacaacc tctcaggctt ttgatttacc tgaagcgcgc
    13261 tggcaactat tgttttaggg acgttcgtgc ggtttagtat gcccagccag ttcaaggaaa
    13321 ttcgctcatg tatttactcg gggagcaacc ggcttacgcc gatcagctga tcaaccgact
    13381 acaaagcatc cccactcaat tgctcgatgg gcttgcacct gctggatcgc cactacagct
    13441 tgaacgtgcg gaagacctgg ccaagattct gccgggcaat cagctattca tcattgagaa
    13501 tggcctgtta catgccgtgg ttgacgaacg cccactgttt tatctgcagg aaggcgatct
    13561 ggttggcctg cgccaaggcc tggacatgcc cagctgccgt tacagcagtg aagagcaact
    13621 aagcctgatt ccctactcgc gcagcgaagt gttcaagcac atctatgcca gcgaacagcg
    13681 ccaggaactt tttatccagt acctgatcgg ccataccgcc ctgctttccg atgccctggc
    13741 acgcttgaag cagccggaaa tccgcccctc caccggcttc cagcattttg ctgcaggtga
    13801 agagctgatc catcagggcg atgtcgctga caacgtcttt atcatcatcg aaggtcatgc
    13861 cgaggcgtac gtcgacgggc acaaggtcgg tgatgtgcaa aaggatgaaa tcttcggtgc
    13921 catggccgtg ttcacccgcg agaaacgcag cgccactgtg ctggccagcg aaccctgcac
    13981 cgtgatggtc attcccaaag agcagttcct cagtctgatg caaagcaacc cacggattgc
    14041 ccacagcctg atcgagagca tggccaagcg catcgacctg ctgaacaagg aagtcaccca
    14101 gctacgccta ccggccgaag cgtcctgaaa cccgcttact gagcaaacaa cccgacctcg
    14161 tgtcgggttg ttttttagga ccccaaacac acgcctcgca ggcagataaa aaagtttgct
    14221 taggggtgtt gacatgaaaa tgagaattgt tattattagc acaactggtc gcgagatcag
    14281 ccgataagct taagagacct tcagtcggcc tcttcagatt atctcctcat caggctaatc
    14341 acggttattg acccggctct tgccgggtct ttttttgcct atcgaaaagt ccgcacagca
    14401 tcctttagaa atcgacctgc gccagccact ccggcagatt gaaatgctgg ctcactcggc
    14461 agatcttgcc gtcgcgaatc tcgaagaaac tcgctgccgt cagctgatag ctctggccac
    14521 aggcgtctgg cagaccatca tcggtaatca ggtattcacc gctcagggtg aattcacagg
    14581 cggcgcgctg cccgcttggt tcggtgagta tctgcaggtt atcgatgctt tcgcggtagc
    14641 agcgctgggt gcgctccagg taatcggcaa aggcttgtac accctgctcg cgcgggccct
    14701 ggttgatatc cagcagcaca tcagcgctga gcagggccag acaggcgcgg ctgtcgcgtt
    14761 cgttgagggc gttgaagtag cgggtaagca aggcgtgggc agcggctggc atcagcggtc
    14821 tccggtgaaa accgcagact acctgctcag ccgcaggcaa atcgggctag ggtcgcagtt
    14881 ttgcgcagtg gtcctgctca ggttgcgctg gcgtgtacca gacgtaatcg gccgactcgg
    14941 cgacaacctg gctgccgacc tccgccagaa tcatcacctg cacaccttca gcagcgccta
    15001 gatcctgcag atgcaacggc acgcctagct cgcggcgcac atggaatgcg ccggcaaaca
    15061 gcagacttgg cgccggcgcg gccaacaggg cttcggccat acgccgatcc cgctgttgct
    15121 gcacggcaag catggcgggc aactgacttt ccggcagcag gttgcaatgc gactcccgaa
    15181 tctgcttgaa cagcgcctca cgcaccagct gcgctgtgga agcctggcct tgcagctgcg
    15241 gcacctgggc gtaaatctgc atgacttcgc ggcgctcaag gttggctgcc agcaacggat
    15301 aaggctgacg caggacatgc tgcaccagcg gcccgtaaag cgaccaagcc caacctggct
    15361 gccaggccag ggcgctaagc atgtcctgcg gcgcctgccc ggccgcgatg gccgcgcgta
    15421 cctgttcgac cttggcctgc tgatccggtg tcagcatttc cagcagcagg ctgccctgcg
    15481 gccgttgcgc tgccagagcc cgcaacagcc agagctgcag cgcgtggtga tcggggttgt
    15541 catgccgctc gccgaccagc accttgggcg cagcagccag acgcgtcagc agttgctcag
    15601 gcgtcagccg cgcgccggtg cgcaaatcga caatctgccc caactcagga tgctgtaaac
    15661 cgtcggggct ctgccaggca ggtaaagctg gtaacgaggc ctggcaggca ctcaacagag
    15721 cggcagccag caataatgca atacgcataa acaagacctt aacgagcaac gattaaagga
    15781 tgaccccgct ccggatgctg ctgcaccagc acttcgagac cgaacacggc ctgcaatggc
    15841 tcggcgcgca gcacttcctc agggctaccg agtgcatggg gtcgaccgcc attgagcagc
    15901 aacaagcggt cgcagtaacg cgcggccaga ttgaggtcgt ggagaatcac cagcactgcc
    15961 gcgccctgct cgacaaaagc gcgagtggcc tgcagtgtgg tgtgctgatg cagcggatcg
    16021 agcatcgacg tgggctcatc gagcagcagc atctgccctt ccccaccagg ccagagctgc
    16081 gccagcacgc gcgccaggtg cacacgctgg cgctcgccac cggataacgc cagatagctg
    16141 cgtccagcca ggtgcaaggc atcggccgcc tccagcgcct gcttgatgat ctgcgcgtca
    16201 cgcacacgcc cgctggcgtg gggcaaacgg cccatgccaa ccacttcttc cacgcggaag
    16261 gcgaagttca gcgtcgaggt ctgcggtaat accgccaaac gctgcgcacg ctcgctgccg
    16321 ggccagtcct gtaaacgccg ctgatccagc cagacatcgc catggctcgg ctgcagctct
    16381 ccacacaaag cgccgagcaa ggtgctctta ccggcgccat tgggccccag cacgccgagc
    16441 acttcgcccg gacgcagctc cagattgata tccgccaata ccgtgcacgg accgcgctgt
    16501 accgccagat tttctgcgcg cagcatcagg tacgcccccg caacaacaga taaaggaaga
    16561 acggcgcgcc aatcagcgcc gtgacaatac caatcggcaa ttcggccgga gccaatgcca
    16621 gccgcgccac cagatcggcc agcagtaata ggctggcacc cgccagagcc gaggccggca
    16681 acagcacgcg atgatccggc ccgaccagca gacgcaccag atgcggcact accaggccga
    16741 taaagccgat catccccgcc gccgccacag ccgcgcccac gcccaaggcg gtgcagaaca
    16801 ccagctcggc cttgacccgc tccacggcaa aacccaggtg tcgcgcctcg gattcgccca
    16861 acagcaaggc attcaacgcc ttggcccgcc gcggcaacca cagcgccacc gccgccgtaa
    16921 ccagcagcaa cggccataaa cggctgtaac tggcgccatt gaggctgccc aggttccaga
    16981 acgtcagggt acgcaaagtg gcgtcatcgg ccaggtaggt aaacaggccg atggccgcac
    17041 cggccagtgc cgtaagtgca atgccggcca gcagcatggt cgccacattg gtttcaccgt
    17101 cacgccggcc caggcgatag accagcgccg tcaccccaag gccgccgata aaagcgaaca
    17161 acgacaatag ataaggtgca aaggcttccg gcaagccgcc aagggccgcg ccgccgacaa
    17221 tcgcaatcga cgcgccgagg gccgcgccgc tggatacgcc gatcaggcct gggtcagcca
    17281 gcgggttacg aaacagcccc tgcatcgcca ccccgcacag cgccagcacc gcgccgacca
    17341 gcagcccgag caaggtacgc ggcaaacgaa tctgcccgag tatcagctcg gcctgggcca
    17401 gatcatcccc aggttgtgcc aggcccagca gacgcaacgc cgccttgagg gtgtcaagca
    17461 gcggcaggct gaccggcccc agcgccaggg ataaccagag cgcgagcgcc agcaacaggc
    17521 tcagtgcgat aaacagtgag cgcggacgaa taacaggatt catggcgtgg gcttagcttc
    17581 ggcgaccaga ggttggctgg caggataaaa ggctgcggac aaggccgcca gcccatccgg
    17641 caaacgtggg ccgagaccac cgaccagcag cgtcgggtcg agcatcagca aacgctggtt
    17701 acgcgccgca cgagtcgagg ccagcgccgg gttctgctgc aacagcgcct cgcgcgcggc
    17761 atcaccggcc agactacggt cggcaatgat taccacctcg ggatcaaggg ccagcagcgc
    17821 ctcggtggac agcgccttgt aaccgctatg gctggccaga ttgcgcccac cggcacgctc
    17881 gatcagccaa tcggccgagg tgtccttacc gcccgccatc gggctaccgc cagcatgccc
    17941 gagcaacagc aacacacccg gcgccgcctg ctgacgctgt gcagttacca gccagtcggc
    18001 ttgctgctgc aagcgctgtt gatacgccgc aaaggcctga tcggcgcgct gcggctcgcc
    18061 taacaaagtg ccgatgcgct gcaggctggc ctgcaggcta gtcagatccg ccttggacga
    18121 gagcatttcc acccgcacac cggcgccgcg caattgcgcc agcaccggcg gcgggcccat
    18181 ctcctcgcta ccgatcagta catccggacg cagggtcaac atgccctcgg cggccaactg
    18241 gcgctgataa ccgatgctcg gcagcgcgcg cagcgactgc ggatgctgac tggtgctgtc
    18301 caccccaacc agacggcttt caccacccag cgccaccacc cactcactga gcgaaccgcc
    18361 ggaactgatc cagcgttgcg gtaacggctc agcggccatc accgaggcac aggacaacag
    18421 gctcgcgcac aaggcgagca gagtattaag gcgtttcatg attggcctct agaagaatgc
    18481 gactgtcctg aatgccctgc tgttgcagca gcgccatcag gtcgagaaat gccgacagcg
    18541 gcgcctgacg atccagtgcc aggtccagac ctgccgtgtt gtcggcgaca aaggcttggc
    18601 tcagctcagt gcgcagcgtg ttgaaatccg cctgctgcac gccgttaaag cgccaactcc
    18661 cttcggcgga cagctgtatt tgtatgcgct cgccgctgcc ggcactcgcc gtgggcaacg
    18721 cctcgcggct ctgcggcaac tgtacgggca gctccagcag cggcgtttgc gcggtcagca
    18781 gaaagaacac cagcacgata aaaatcacat cgagcagcgg cgtcagatcg ggcagcagat
    18841 tgccttggga gctaccggca gccggcagac ggatcacggc aacacccact gcgcggctgg
    18901 ctgattagca ggcagttcca gctccaggcc atccaatgcc agctggcagc gattgagcaa
    18961 cgcctgcacg ccttccagat aacgctcggc ccacaaggca aagccctgcc ctgccgccag
    19021 cgccggaatg gcgatcagca agccccacac cgtggtgtag agcgcctgcc acaggccatc
    19081 ggcgagcaac gctggggtta ccgggctgtt ctgcccggcg atattggcaa acatctcgag
    19141 catgcccagc accgtaccga gcaaaccgag catgggcgcc agcatgccga tcagctgcag
    19201 caaacgcagt tgtcgattga ggcgctgacg ctcttccagc aaccagcagc cgagcagctc
    19261 ttcgcgctgc accgccggca aggcgctgtg tttgagcaac aacgccaggc catagcgcca
    19321 accacgcgcg cgacctttgc agtgctcgcg ctcacaggtg cggcaggcgc tgactgctgc
    19381 acgcccggcg gcagtgctca gcggctgcaa acgcagaaat acgctaaggc gctccagaat
    19441 cagtgccagc gccagcagcg aacatagtgc cagcggccag cccagcggcc ccatcgcact
    19501 ccaccactga ctcatctcag cactccagcg ctggcgcgct ttcggcaagg gcgcgccagt
    19561 cttcaagctc cgggatgcct ggtttgcgtg cgccgaacag ttgcaccacc aattcgccat
    19621 tagcatcgaa ggcttcccag ctggtgatca caccatcagt gctcggcttg cgcacccgcc
    19681 acagctcggt cacgccagtg gtctgcaggt gcagattgaa ctcggggtcg agcacgttga
    19741 accaggtatc catccagcgc aggttgcgta ccgggccgga gtgaatctga atgcagtgac
    19801 gattaccgac aaacaccatg atcggcacct cgcgcgcacc ggcctgctcc agcagtttgg
    19861 gcagttcgcg tacggcaagc ggctcggccc actcactgcc ggccaggcgc agcgcctggg
    19921 ttcgtgtggt gccgtgcttt ttcagcaggg cgaaaaagtg gtgagtgtcc ttgagggtcg
    19981 accaacccac gcgcagggcg tttacatcca cctgagcatc ggcctgaacc ggcacggctt
    20041 gtggaagagg caacagctcc agctcggcac tttgctcagc catgcggaaa ctctccagca
    20101 gtggcgccca ggcacttagc tggctgtcat cggtaagaaa caccttgtgc actgcagtgc
    20161 cttggcggtc gaacacctga atgctgcgct gggtgccttt gccggtctgc tcctcgatgg
    20221 caaacacact ggcccagccg ccgaggaaca gccgcaggtc gatatcagcc gacaccacca
    20281 ggcccatctg accattaccg gttaccgtca cttcacggta ataacctttg cgctcatgca
    20341 cgcaatgctc gttgcgggtc agcgccatga cataacccag ctcgccgagt gccggcagca
    20401 actgcgccca atccggttgc agacgcacag cgtcgatatc gaggcggctg gccgccagct
    20461 cggcctcgct gacggccaga cgctcggcag catcgcgggc acgcaggcgt ggctgctcgc
    20521 tgcgcagcac ctgccaggcc agatacagat cattgttggg cgcgacggat tgggtgtgcg
    20581 tgctcatgga aaactcctca ctgattaatc aacggctcgc cgtcaaggcg aactgaatag
    20641 ggatctgtac acggctaggc acggcctggc cgcctgtggt ctcagggcga aagcgccact
    20701 tggcaaccgc ctccagggcg gcgcgatcaa ggctgtccac ccccgaggag cgcagtacat
    20761 tcaggctgcg ttgctggccg cgctcatcca ggcgcacctc gaccagcacc accccctgct
    20821 gattacgccg gcgcgcctgg gacgggtaac tcggttggcg gggcggctcg cggaaactcg
    20881 gtttgctgct caacacctca gtggccggct ttgctgcaac cgacttggcg ggcgcggcct
    20941 gagccgccgc aaccaccgca gcggactgac gggttgtatg gctgggctgc gctggggcgg
    21001 gctgactttg cgctggttgc gccactaccg cagactttgg tttggccact gccgcctgcg
    21061 ctacgggctt ggctaccggg accttgctcg gcaccaccgc ctggcgttgc ggttgcggtt
    21121 gcggttgcgg ttgctcaggc agcttggccg gtgccggcgc gacctgttgc agcactgcag
    21181 ccaggcgtaa tacctgaggc gcttcgcgag tttgcgccgc actcaacgca ggtggttgcg
    21241 cttcgccatg caacagccag ccggcggcat gcagcgccag cgacagcagc agaaacagcg
    21301 acaaacgcag catgcgatta cccagtcaaa tcgaattaaa tctatttgat aattatttgc
    21361 attcgcgtgt caatgtcgtt tagctttgcc gccgatgcca acccggtggt cacagaaacc
    21421 acctgcacga tcctggagtc cccatgcaca gaagtcctcc attcgcccgc cgcccctggc
    21481 ttgccttgct attgctcagc ccgtcgctgg cccttggtgc agaaaaagcg ccgaaccagt
    21541 tcgataccgt gaccgtcacg gccacccgca ccgaacagac cctcgatcag gtgccgagca
    21601 cggtgtcggt acagaccgaa cggcagatcg accagcagaa catcaagaac atcaaggatc
    21661 tggtgcgcta cgaacccggc gtgtcggtcg gcggcagcgg tgaccggttc ggcctgtccg
    21721 gcttcacgat tcgcggtatt ggtggcaacc gcgtactgac ccaggtggat ggtgttggta
    21781 tgccggacgc gttctccttc ggtggtttcc tcaacgccaa acgcgattat gtcgacctcg
    21841 acaccgtcaa acaggtggaa atcatccgtg gccctgccag ttcgctgtat ggcagtgatg
    21901 cgattggcgg cgcggtgagc ttcctgacca aggatgccgg cgactacctg gacgaaggcg
    21961 atgacgctta cgcccgcctg aaaaccggtt atgacggcag cgatgacagc tggcagcgca
    22021 gcgccacctt cgccgcacgc ctgggtcagg tcgatggttt gctgcatctg ggccggcgca
    22081 gcggtcaggc cacggacacc cacagcggtc agggtggtat cggcgcaacg cgcgagcagg
    22141 ccaacccgct ggattaccgc accgacaacc tgctgagcaa attcggctgg gattacagcg
    22201 acagcggccg cctgcagctg acctacgagc gttaccagga cgacaccgac acccgcgtat
    22261 tgagcaacta cagcaatacc gcaaccatcc gcacccagga tgctgaagac cgcgtcgacc
    22321 gtgaacgcta ctccctcgcc catacccagc aattcgacac cttgctggtt gaccaaattc
    22381 atagccaact cacctaccag gacagccaaa cccgccagca gaccttcgaa gaccgcgtac
    22441 tcgccgggca gccccgctac cgtactcgcg actcctacta cgaagaaaaa ctctgggcgt
    22501 tcaacaccaa gctggacaag gccttcagca tcggccgcac ggaccatgca ctggtctatg
    22561 gcctggatgt caaacagctg aaaaatgcgg acctgcgcga aggcggcgaa accgtactgg
    22621 ccacaggcgt caccacgcct gttctaccca ccagcgattt ccccgaccca accaccaggg
    22681 aatacgccct gttcgcccag gacagcatca gcatcggccg ttggaccctg ctaccgggcc
    22741 tgcgctacga ccactatgag atgagcccac acgtcaccaa taagtacctc aatagccagg
    22801 ctagcgatgc caaccctagc gacttcaagg attcggcgct ctcgcccaaa ctcggcgtga
    22861 cctaccagct cgacgacgcc cacagtgttt acggccaata cgcagccggt ttccgcgcgc
    22921 cccaggccat cgagatcttc ggcgagttcg ccaacccggg catgtaccgc accctggcca
    22981 ataccgacct gaaagcggaa accagcgaca gcttcgaact gggcctacgc ggccaatacg
    23041 ccatcggcag ctttggtgcg gcgctgttct acaaccagta cgacgacttt attgagcagg
    23101 tcagccggcc ctccagcgtg ccgggcttcc ccttcggcga gttccaatac gtcaaccgtg
    23161 atcgcgtgac cattcgcggc gcggaagcca aaggtgagct gttcctcgat caactgggcc
    23221 tgccggctgg ctggaaagca cgcggcagcg tggcttacgc ccgcggcaag gatgaaggca
    23281 caggtcagcc gatcaatagc gtcgacccgc tcaagggggt attcggcctg ggctacagcg
    23341 agccgagcgg caagtttggt ggtgagctga gctggaccct ggtggcggcg aaaacccgcg
    23401 tcgacatgac ccaaaccgct aaccatgagc agacctcggg ttatggcgta ctcgacctca
    23461 acggctggtg gcagatgacc gacgccttcg cagtgaatgc cggcctgttc aacctgaccg
    23521 acaaagagta ctggcagtgg ggtgatgtgc gcggcctgac tgaaaacagc ccaagcatcg
    23581 gccgcttcag ccagccgggc cgccacgcgg ccgtcaatct ggtctgggaa atctgaccca
    23641 gcaacacgtc taccgcgcta tcaactagcc gcgaaaaaga gttccacttg gcgcaaggat
    23701 gcgccctcct ctttcacttc cacgccaggg cggcataatg ccggcctgtc ggggagggtc
    23761 ctatgttgcg tttatgtgca ccggatgaag tggccgaagg ccagagccgg gtctttgagg
    23821 ttgcgggtga gaagctgttt gtcgtgcgca aggacggcca gctgtatgcc tatcgcaatc
    23881 gctgcccgca ccgtggcatc ccgctggaat ggctgcccga ccagtttctc gatcacagcg
    23941 ccagcctgat ccagtgcgcc acccacggcg cactttttct gatcgaatcc ggcgagtgcg
    24001 tcgccggccc ctgcgccggg caatcgctgc aagagctcac catccgcgaa gatgatcagg
    24061 gtatctgggt cgagctaaac gaagcctaaa agctcttagt gcagcgataa ggttcaactg
    24121 tcgccgacct gtaggagcac gccatgcgtg cgaaatgatc tgtgacgcga cactagagtc
    24181 cggctgcgtg gaggcgcttt gcatgctcaa caaacagctt taccggctca gcgcctttgc
    24241 cgaccaggcc aaaggtctga ttgatgatgt cgaagtgatc cagcgggtac tgatcaccaa
    24301 tcaccgtgcc caggtgtgag ctgtagcgcc cgaccatgcc gtcgcactgc ccaacctcac
    24361 gctgaaaggt cttggcaaac atccggcagg cgacatttgg cccatcaaaa cgattgccgc
    24421 ctttatcagt gatgcccggc tgaatggtgc cggaccagga gtagtagcgc acaccattga
    24481 cctcggccgc gccctcaccg ccccaaactt ccggcaaacc ctgcggatac tgcgcattga
    24541 aggccgccac accctcactg gtcagcgatt gttgcgccgc tgccacatcc atcggcagtg
    24601 gttcgcctct ataaccgcgc tccagccagg ccatgcagcg ggccacacca cgcagcaggc
    24661 gcgtcaacag acgctcgcgc cagctgccgt gcggggcttt gcgtttgaga aagtccgcca
    24721 gttccgaacc atgattaggc ccggctaccg aggtcacgga tgccacccac tccggtttta
    24781 gcgcggccgc atagcgcgcg gtcagtgcac cctggctatg accgatcaga tggacctttt
    24841 cggcgccggt ctgctgcagc acatcggcga tgcgctgcag cagttgctcg ccgcgcacct
    24901 cggtggaatg caccgaggac accagcaccg ggaagactgt tgcaccgatg cggcgcagcg
    24961 ccgtgacgat gccgtaccag tagggataac cgagcaggct gacaaagccg agcaaacctg
    25021 gcaccaagac caggggataa cggggtgcag aagatgtcga catcgcaaac catccttgtg
    25081 tcataccgcc gagaaagaaa gtgcagaacc acgaagcatg ccaatgccgc tcgtgggaaa
    25141 actgggccgc ctgacatata gtcccggctt ctctttgcgc tgccatgacc atgcatacca
    25201 tcgaacggat ttacccgaag gatctcaacc cgcttaaccc ggatgaccag gccagcctgc
    25261 agattcatat gcagcgttat gaattcgccg ccgagcatct cagcggccag cgcgtgctgg
    25321 acatggcctg cggctgtggc tacggcacgg cgctgctggc cgagcgtaac ccggacaagc
    25381 aggtcaccgg cgtcgacatc gacccggcgg ccattgccta cgcccgcgag cattaccagc
    25441 tggccaatct gcgttatgaa tgcgccgatg ccgagcagtt cagcagcgac caggggttcg
    25501 attgcatcgt cagcctggaa accatcgagc atctgccgcg cccgcacgta ctgctggcca
    25561 actatgcgcg tttattggcc gagggcggca aggtcatcgc ttcggtgccg attaccccaa
    25621 ccctggacgg caacccccac cacctgcatg acttcagcaa acgcagcttt tatgcactgt
    25681 tccgccagca agggctgcac gcgcagcagc acttggagca gattcagtgg tggcagttca
    25741 aaggtttatt ctcgaaacag gtcgatcaac agaaacagca ccgcagcgaa ggggtcggca
    25801 atgctgtgct gctgtattac cgcaagcgcc cgctgtacct gttcagccgg ctgtattcga
    25861 tgctgcgcta cggctttagc aaccgttacc tgacttgcgt gtttacgcgc cagccggcat
    25921 agcctcggca gggcgccggc ggtagatcca aattcgccta agcaccgtcg aaaattggct
    25981 ccacaaactg ccgttgttgt agacctggcc ataacgctcg gcgatctcgc gcacttccac
    26041 ggccaacgcc gcatagcgcc gcgccggcag atcaggaaac aggtggtgtt caatctggtg
    26101 gctaaggttg ccggtgagga tatggaacag cggcccacca ttcagattgc tggaaccacg
    26161 tagctgacgc aggtaccagt gaccacggct ctcgccgacc accgactcct tgctgaacac
    26221 tgccgcctgc tcggtaaagt gaccgcagaa gatcaccgta aaggtccaca gattgcggat
    26281 caggttggcc acaacattac cggccagcac cgccagggca ttggcaccga tcagcaaggc
    26341 caacagcggg aagaacaggt aatccttgcc ccactgccgc aggatcttgg cgttgaactg
    26401 tttgatcagc gggcgtactt cagccttgct cagcttgccc ttgtagacct tgtccagacg
    26461 caaatgctgg atcgccaccg agtactggaa tagcagcgcc tgaatcatca cccacagcgg
    26521 ctgccaacgg taaaacggct tccagcgctg ctcgggaaac agccgcacca cgccataacc
    26581 gacgtcatcg tccatgccca gcacattggt ccaggtgtgg tgcacatgat tgtgggtgtg
    26641 ccgccagaag tcgctgggcc cggcgatatc ccactcatag ctgcgcccgg caaactcggg
    26701 gtcattcatc cagtcgtact gaccgtgcat gacgttatgg cccagctcca tattctccag
    26761 aatcttgccc aggcccagca gcagcgtgcc gagcagccag gtcggcggaa accagccgag
    26821 catcagcagg ccgcgcccgc tccagtaaca caagcgcacc gccgctcgaa tgcggcggat
    26881 atagcgcgca tccgtctcgc ccagatcagc cagggtgctt tggcgcaggg cgtccagttc
    26941 gttgccgaag gcgttcattt catccatgga cagttcgcga tcaccacgca tggggattcc
    27001 ctcgcaagtt aaagatcaat ctcgacatcg gtgtgcggcg cgctaacaca taggcggatc
    27061 ggctggcccg gctcgctgaa caacacgccg ttgcgcaaat cgcgcaccgt gccgctaagc
    27121 aaggtgcagg tgcagctggc gcaaataccc tggcgacagc catgcaccgg ctgcaggcca
    27181 tgggcctcag cctgttccag caggctgtgc tgattattag ccgtcgtctg ctgatggctg
    27241 cgggcaaacc ccaggcgcac ctggcgcagg ctgacgtcgg cgcgcagcac gggcgcagta
    27301 aaggcctcga cctgcagcgc accaggcaat ctggcggcct gccaccactg ctgcacctgg
    27361 gcgacaaacc ccgccggacc gcaggccagc acacggcgct gatccagctg cgtcacctcg
    27421 gccagatgct cagcggtaaa acgtccagct aacccttcag gatcagcacc ggcggcggtc
    27481 agtgaccagt gcacctgcag attgccgtgc tgcgcctgca aggcctgcag ttcggcgaca
    27541 aaaccgcgct ggccctgctc gcgcacataa tgcaacagcg tgatcggcgc gctaaaaccc
    27601 tgagccaggg cggcgcgcaa catgcccagc agcggcgtga tgccactgcc agcggcgagc
    27661 agcaacacac cctgttgcgc ctcaggccaa ctcagctcgc catacaccgg ccccagctcc
    27721 agcacatcgc ccaccgccag atgctccagc aggtagggcg atactcgccc acccgcctga
    27781 tgcttgatcg cgatctgcag gctgccatcg gcttgcaccg cagtcaggct gtaactgcgg
    27841 ctcaggcgta cgccatcacg ttcgagatac agctgcaagt gctggcccgg ctgcgcgccg
    27901 cgccagttgc cgttggcctg cagggtcaat tcgagcatgt cagcggcaac ccactgacgc
    27961 tgggtgacgc gcgcaaatgc gcgattcaga cgcagcgccg gatgcaacag cgctaacccg
    28021 gcatcgacat cactttcccg caaccagcca tgagcgacca gcacgcgcag cggatagagc
    28081 aggcttgcga gcccgcgtac ccaagcaaaa ggaagaaggg ccatggcaag actccagtga
    28141 acaattgttc actagactgg cagagcatat ttattcagtc aacactcgta cactcaaagt
    28201 tagatgagta accgcggttc gacgcaagcg aagcgcattt gctagagtgc ggcgctgatt
    28261 gattgatgca gagccaggaa gcttgaaacc actacaaccg cgcgccgaac aaaaactgca
    28321 aacccgccac gccttgatgg acgcagcgcg cagcctgatg gacagcggcc gcggctttgg
    28381 cagcctgagc ctgcgcgaag tggcgcgctg cgccggcatc gtgccaaccg gtttctatcg
    28441 gcacttcgag gatatggacg ccctgggcct ggcgctgatt gccgaagttg gggaaacctt
    28501 ccgcgaaacc attcgccagg tacgccacca cgagttcgaa ctgggcggcg taatcgatgc
    28561 ctcggtgcgc atcttcctcg acgcggtggc ggccaaccgc tcgcagttcc tgtttctcgc
    28621 ccgcgagcag tacggcggct cgctaccggt acgtcaggcc gtaggcgcat tgcgcgaacg
    28681 catcaccgcc gacctggtgg cggatctggc ggccatgccg aaactgcagc acttcgactc
    28741 cccggcgcag aacgtgatcg ccgacctggt ggtaaaaacc gtatttgcca ccctgcccga
    28801 actgattgac ccacccgcag cgcccctgcc gatgcacgcc tcccccgagg tcaagatgat
    28861 cgaacagctg cgctttattt ttatcggtgc caagcattgg cagggtctcg gccagactcg
    28921 cgactgatcg tcgatcgcct tacgcaccaa gatcgcgcac aaacccagtg tacgcaccaa
    28981 accaaaaccc gccacgctcc aatttcagcc acaccacacc cacgcggccc gctctgcgtg
    29041 gcgcaacggc agcttggcaa ggcccttgct ctataaccgg cattgccctg ccggaaactg
    29101 ccccatgttg gtgatccacc gccgtattgc cgcccagccg tgctgggatg ccgaactcga
    29161 actgagcttc gaggcccgca gcaaaagccg tctgcgctgc ttcagccgca gcggcgagga
    29221 tgtcggcctg tttctcgaac gtggccaacc cgccttgcac gacggcgaat gcctgcaagc
    29281 cgaagacggt cgaatcgtgc gcgtggttgc ccgaccggag ccgctgctgc atgtgacctg
    29341 caccaaccct ttcgaactga tgcgcgctgc ctatcacctg ggcaaccgcc atgtcgccct
    29401 gcaactcggc gatggctggc tgcgcctgcc ggacgactac gtgctcaagg ccatgctcga
    29461 acagctcggc gccacagtcg agctgatcga cgcgccctac caacccgagc aaggcgctta
    29521 cggcggtggt catcaccact cccatgccgg cgatgctgaa ttcagctacg ccccgcgcct
    29581 gcatcaattc ggtgcacgca agtgaataaa gcctggcagc tattgaggct cgccagcccg
    29641 cagctgccga atagccagcc ggttacagcg cactcggcaa cagggcttat cagtcacggc
    29701 ccggtacatc cagatgaata aggcctgggc gctactgaga ctggcaagcc cccaattacc
    29761 tattggcggt tatagctatt cccagggcct ggaaatggcg gtggaacagg gcctggtcga
    29821 taacgcagaa agcgccgagc gctggattgc cgatcagttg ctgctgaatc tcgcgcgctt
    29881 cgaagcgcct ttgctgctcg cccactgcaa ggccgccgcg agcgaggact gggcacagct
    29941 gcgccacctc gccgaacaac aacgcgccag ccgcgaaacc cgcgagctgc gcctggaaag
    30001 ccggcagatg ggttactccc tgcagcaact gctgagcggt ttgccggagc tggatcagcc
    30061 ggcgcagcaa ctgtttagtg agctggccga acccggcctg gccgttggct gggcgctggc
    30121 cgcgcgggcc tggcagatcg agccagagga tgccctcgcc gcctggctct ggggctggct
    30181 ggaaaaccag ctggcagtgc tgatgaaaac cctgccgctg ggccagcaag ccgcccaacg
    30241 tctgacctcg aaactgctgc cgctgctcga tcaggcgcag cgccaggcca gcgaactgca
    30301 accacaacac tggggcagcg ccgccttcgg cctggccctg gcgagcatgg cgcatgagcg
    30361 ccagtacagc cgtttattcc gttcttgatg ataaggaaga cttgaagatg aacagccaac
    30421 ccctgcgtat cggcatcggt ggcccggtcg gctccggcaa aaccgccctg accctggccc
    30481 tgtgcctggc cctgcgcgag cgctacaacc tggcggtggt gaccaacgat atctacaccc
    30541 aggaagacgc gcagtttctg gtgcgcaacg aggccctggc gccggagcgg atcatcggcg
    30601 tagaaaccgg cggctgcccg cacacggcga ttcgcgaaga cgcctcgatc aacctggaag
    30661 cggtcgacca gctcaaccgg cgctttccgg ggctggacct gatcatcgtc gaatccggcg
    30721 gcgacaacct ctcggccacc ttcagcccag agctatcgga cctgaccatc tacgtgatcg
    30781 acgtatccgc cggcgacaag ctgccgcgca agggcggccc gggcatctgc aaatccgacc
    30841 tgctggtaat caataaggtc gacctggccc cgctggtcgg cgcatcgctg gaagtgatgg
    30901 aacgcgacac cctgaagatg cgcggcgaca agccgtttgt gttcagcaac cagaaaatcg
    30961 gccagggtct tgagcagatc atcgccttta tcgaacgcca cggaatgctc accgccgcgt
    31021 aggagcgggc catgcccgcg aacacttcac gggcatgggg ctaggcgcca ccgccgctcc
    31081 cacggaaatc caagcctcac gatcaatcaa ggagacccac catgaacctg cgtaaagccc
    31141 tgttcgccat cgccctgttc ctcagcccag ccatggcctt cgcccacgct gggcacgacc
    31201 actccgggct gctggccggc ctggcgcacc cggtattcgg cctcgaccac ctgctggcga
    31261 tgctcgccgt cgggctgtgt gccgcgcagc aagccggcgc gtcgcgctgg gcgctgccgc
    31321 tgacctttgt cggcagcatg ctgatcggag gtctgctggg ctttaacggc ctgcagattc
    31381 cgctgatgga aaccggcatc gccgcctcgg tactggcttt cggtctgttg gtcgccgtgg
    31441 ccattcgcct gcctatggtt atctccattg ccctgaccgc actgttcgcc ctgactcacg
    31501 gcgtcgccca cggcctggag ctgccggagc tggccagccc ttggggctat gccgtcggtt
    31561 tcgtggtggc caccgctgcc ctgcacgcca gcggctatgc cctggtgcgt ctgctgcccc
    31621 aggccgctgc accggtggtg cgcgtcctcg gcgccgcctc ggcagtcacc ggtgcctggc
    31681 tgctggcagg ctgagttgct ggctcgggtg gacgaccaac gtccacccag atccgccacg
    31741 ccagcctccc ctgaccgcaa aggtcgctag gctggcgcat ttcgcctctc gctcagcagc
    31801 catggctttg tcatagtcga ggcgtattaa accccctcca tcattgactg ccctggaccc
    31861 tgaccatgct gcgcgtcgcc cgctttctat ctgccccgct cttgttgcta gccatcgccc
    31921 tcggcgtact ggccctgacc agcaaaccgg cccagccggc ggcggtgctc agcagctttg
    31981 ccgaacaacc gctggttatc gctcaccgtg gcggcaaagg cctatggccg gagaacagcc
    32041 tgtttgcctt ccagcgcgcc agcgccctag gcgtggacat gctagagatg gacctgcacc
    32101 tgtccagtga tggcgagctg gtggtgatcc acgacagcac cctggagcgc accaccgatg
    32161 gcgagggccc ggttgccgcg cttagcctga cgcagctgca agcgctggac gccggttacc
    32221 gctggagcgc cgatggcggc cagagctacc cctaccgtgg ccagggcatt cgcatcccac
    32281 gctttgccga ggtgctcgac agctttcccg acacccccaa agtgattgaa atcaaggtgc
    32341 cggatgtcgg catggaagcg ctgctgtgtg atgcccttga ggcgtaccag caacgcgacc
    32401 tggtgatcgt cggcagcttc tatgaccgca gcctacaact gttccgcgaa caatgcccgg
    32461 gcgtggccac ctccgcaggg cctggctcag tacgcctact ggtggcgctc aactgggtcg
    32521 gcctgggcag cctgctgtcg ccctcctacc aggcgctgca gattcccgaa gctcacggcg
    32581 gcctgccagt cgccagcccc agcctgctca agaccgctgc acaacgcggg ctgaacgtgc
    32641 agctatggac catcaacgaa cagccggaca tgcgccgcct gctcgatcag ggcgcccatg
    32701 cgctgatcac tgactacccg gaccgcgccc tgcaggtgct ggggcggtca acccagatca
    32761 gcgccctgga agactaagag ccttttacga tctgctgcgc gtcggccctg ctgcgttgaa
    32821 aacaagctcg gaatgctcat ttacagctcg taaactccgc ttcctcgcct gttttcgcct
    32881 tgcagtgctc tagctcgcga gatcgtagaa aggctctgac cactcgccac tgtataaaaa
    32941 acaacctacc gcgttcagac cacgcctgct gtggcctgaa cgcatgcgtg cgcagcttat
    33001 ccacagatcg ctccacagca acctgggata agttatccac cgtgcgccgc agcactgcgg
    33061 caaacccttg aaataacgcg attattgctt gactcggcag agaggaaatg acgtatttcg
    33121 agacctcggc aagctgctca aaaatcgatc agaacgcgca aaaccaggct ttataagcct
    33181 tgcaggcagt cataccagag ttatcaacag ctctacccac agtagtcgtg gacaacttta
    33241 gcgctacccc ataacgtcct gaataaccag gctaacctgt tgttttaacg gtaaataacc
    33301 gactgatcaa aaagcgtaca aaggccgtaa gcagccatac gactaagcct gcaagcgcct
    33361 acgcccacct tatccacagc tcgctccaca gtctttgtgc acaactaacc gtgcgatgcc
    33421 gcaagtgctg tgcataaccc cgaagcggct tccggctatg acccctatcg agacaatcta
    33481 cagcccagcc gcagagcaca ggcgtaacgt cactccactc accaaggaga tacgcccatg
    33541 cccctcaatg acctattacg cagcctgctt gccgcctatg cctgtggatc gtgtggcaac
    33601 agtagcgact gagctgcacc gccgattttt tcgtgcactg cggcaaacct gggtttgccg
    33661 cctatggctg atgcgtgctg actcacgtat aaatctgcgc actattgcct acagatctgc
    33721 ctgcagttct gccaggcgat tcgccccaga aaccgtagaa aggaatccga cccatggccg
    33781 gcgctagcct gttaaccctg ctcgatgata ttgccgccgt tcttgacgat gtagcggtaa
    33841 tgaccaaagt cgctgccaag aaaaccgccg gggtactcgg cgacgatctg gcgctgaatg
    33901 cccagcaggt cagcggcgta cgcgccgaac gtgaactgcc ggtgatctgg gcggtggcca
    33961 agggttcgtt caagaacaag ctaattctgg tgcccgccgc cctgctgatc agcgccttcg
    34021 cgccctgggc ggtgacgccg ctgctgatgt ttggcggcgc gttcctctgc tacgagggct
    34081 tcgagaagct cgcgcacaaa ctcctccaca gcaaagccga ggaccaggcg cagcatgcag
    34141 aactgctgca cgccgtggct gaccccgaag tggacatgct ggcgttcgag aaggacaaga
    34201 tcaaaggcgc gatccgcacc gactttatcc tctccgcaga aatcatcgcc attaccctgg
    34261 gcaccgtagc cctggccagt tttggcaaac aggtcgcggt gctgtccgcc atcgccctgg
    34321 tgatgacggt cggggtttat ggcctggtgg cgggtatcgt caagctggac gacgccggcc
    34381 tctacctcaa ccagcgtggc ggtgaaggcc tgggcggccg cctgcagcgc agctttggcc
    34441 gcggcatcct gtttaccgcg ccctatatga tgaagggcct gtcgattctc ggcaccgcgg
    34501 cgatgtttat ggtcggcggc ggaatcctca cccacggcgt acatgccgcc caggaatgga
    34561 tcgaccagct ggcgcagagc gtggcggcca gcagtgctct gctcgcggcc ttgctgccga
    34621 ccctgctcaa cgccatcgct ggaatcatcg ccggggcagt ggccctgttg ctggtcagcc
    34681 tggcgacacg ggcctggaaa gcgatcaagg gctagtgcgc cgcaaaccgc gagacgctgg
    34741 tgcgcacggc gcaccctacg gtttagcggt ccgccagagg cagcaccgtc acctgcacct
    34801 ctgggtcatg gctaccgccg ccgagaatca ccccgcgcag cggcgagacg tcggcaaaat
    34861 cgcggcccca agccagggtg atgtgctcca gcgccgggcg gatattgttg gtcggatcga
    34921 aatccaccca gccctgctgc gggcagaaca ccgacaccca ggcgtgcgag gcgtcggcac
    34981 cgatcagccg cggctggccg ggtggtggct gggtcagcag gtagccgctg atatagcgcg
    35041 ccgagaggcc catggagcgc aggcaggcga gcatcaggtg ggcgaagtcc tgacacacac
    35101 cgcgacgctc ctccagcacc tgcaacagcg gtgtcgccac ctgggtggcg gcggcatcga
    35161 agctgaactc gctgaaaatc ttctgcatca acccatccac cgccgccagc aacggccggc
    35221 cggggctaaa gcagtccgcc gcgtaatcag caaacacctg cttgaggtgc acatagggcg
    35281 actcgaaacg aaagcgcgtg gcatccagca cgccggacag cagcgcctga ccgctgtaac
    35341 gcaacgccgt gcaggcctcc tcccagggcg gcgaagcctg cagatccagc gccgggcgcg
    35401 ccagcacctc gacctgcaga cgcgcactga ccttgagttc gtcatgtggg cgctcgaata
    35461 ccagacgggt cagcgggtta ccgaacacat ccaacgcatc ttgacgcttg cagggctgag
    35521 ggctgaccaa cagctgctgc tggtgattgc gctgccaggg gcacgcgcgc ggccacaggt
    35581 gcgccagttg ctgggccagg gacaccgggc tggcgtagcg gtaatgggtg tcgtgaagga
    35641 tctggtagca ggcgctactc atgaggattg cgtccgctgg ctggcgtcga catgcacaaa
    35701 gaagcgcaat cctaatcgat cagaaatctc cgcgctggcc tggctgatgt tttccagcaa
    35761 gccagccaga ccatccaaca cttcctgcac actggtcgaa ccgaacaggg ggttttccag
    35821 gctgccgagg ctaaagcgcg ccagctgccc ttccaggtgt ttcaaccggc gctcgctggg
    35881 cagctcgaaa cgctcgccca agcgcgccag cgagcgcagc agggtgcgca cctgaaacag
    35941 caccgcatgc gggttctgct catccaacag caacaggtcg agcaccggta tcagctgcgc
    36001 ggaagccagg taacgggtgc ggtaagtgat gctgctgttg cccagttcca gcagccactc
    36061 cagcgcggac tggtcctgcg cggcgctgct acgcagaaaa ttcgccaggc tgtcgcagag
    36121 aaactgcaga cgctcgatac agcggccgat catcagaaag cgccagccat catcgcgagt
    36181 catgtcatcc agggcgaaac ccgacaacgc cgccaacgac agcagcaggc ggtcgagaaa
    36241 atccagtagt tcgccaaagt cggcctgctc cgcatccagg ccctgggctt cgcgctgcag
    36301 ttcgagcagg gcctgccagt tggcctggga cagcttgccg cgcacgctgc cggccaccca
    36361 ctgcaggcgc tgcaggttgc cgcgcaggct gtccggccag tcctcgccaa gcagcgcttc
    36421 aagcaggcgc tgctgcagtt cgccgctttc ggcatccggc agcaggccca ggctctcggc
    36481 cagactcagg gcggtctgca atgcctgcgg gtcatcgtcg tcatccacgt aacgcgccag
    36541 catgatgcgc agcagccgcg caccgtcctc gcagcgttcg ctgtagcggc caaaccagta
    36601 gaggttttcc accactcgcg aaggcagata ggggtcgcta cgcactaaat cggccacccc
    36661 cagcggacgc aaccattgcc agggttcgcc accggcatgc cgttcaccca gcacccaggt
    36721 gtccttgctg gcaccgccgc gctgcatcga caccacttcg gcatcggctt cggcagccac
    36781 tcgggtcagg ccgccaggca tcacccggta accctcggcg ctggccacgg caaacacccg
    36841 catgccgatg gcgcgcggcg ccagctggcc ttcttcaggc tgccagaccg gcgcctgcga
    36901 cagttgtgcc aacgcctggg cgacataggc ataaggccgc gccttgaggc gttcggccag
    36961 ctcggcgcgc tgcgcctcgt ccaaatcacg gccaaacacc ggggcaaagc tctgcgaggg
    37021 aaagctggcg cgcaccagca gctcgtcgag ctgttccagg gctttgtcca gcaccggcgg
    37081 ctcgccgcac caccaactgg cgatgctcgg cagcagcagt tcttcgccca ataatttttc
    37141 cgcgatggca ggaagaaagc ccggcaagcc gggggattcc agcacgccac tgccgagggc
    37201 gttggccacc agcacattgc cctggcgcac cgcttcgagc aggccgggca cgccgagggc
    37261 cgagtcggtg cgcagctcca gcggatcgca gaagtcatca tccagacggc gcaaaacggc
    37321 atgcacgcgg cgcaggccgg ccagggtttt caggtagacc ttggcgtcgc gcacggtgag
    37381 gtcgctgcct tccaccagcg ggtagccgag ctggcgcgcc aggtacaggt gctcgaaata
    37441 gctctcgttg aaacgcccag gggtgagcag caccaccagc ggcgtttcac taccggccgg
    37501 ggcctggcgc gccagggtgt cctgcagggt gcggaagaaa ccggccaggt attgcacgcg
    37561 cagatcacgg tacagctcgg gaaaggcgcg cgagaggatc tgccggtttt ccagggcata
    37621 accggcgcca gacggcgcct gagtacggtc ggcggtgacc caccagcagc catccggcgc
    37681 acgggccagg tccaccgcat acaggtgcaa gtaggtgccg ccaggcggct gcacaccctg
    37741 gcagggccac aggtaattgt tgtggccaaa caccagctca gccggcaaca gtccgtcggc
    37801 cagcagctgc tgcgggccat acagatcggc cagcacggca ttcagcaaac cggcacgctg
    37861 cgccacaccg gcggcgatct gctgccactc ttcggccgga atcaggttgg gcagcaggtc
    37921 cagctcccac ggccggtcgg cgccatcggg gtcggcgtag acgttgtagg tcacgccgtt
    37981 ttcctgaatc tgccgcgcca gcagggcctg gcgctgcgcc agctgcgccg ggctgctgcg
    38041 ctgcaaacgc tccagcaaca ggcgccagtg cgggcgcacc ttaccgtcgg cgtcgaacag
    38101 ttcgtggtaa gccccggacg tcagggggta gtcggcaagc aggtcgggca tggcaggctc
    38161 gataaaaggt tctggtgttg cgcagatgca ggagccaact cgccggctcc tgcaatcgac
    38221 ccaggctagg tcaggcgcag atccagggtc atgggcattt cattattatt gatcggcgcc
    38281 agtgtcgggc gtttgcctgg gctgtggccc agacggaaga agcgcgccag acgccgactc
    38341 tcggcctcat aggcgtttac cggcaggctg tcgtaattgc gtccaccggg atgggcgaca
    38401 tgatactggc aaccgcccaa agagcgactc atccagctat ccaccaggtc aaataccagc
    38461 ggcgcatgca cctcgatggt cgggtgcagg cagttggccg gctgccaggc gcgaaaacgc
    38521 acgccgccga caaactcgcc aaccttgccg gtaggtctca agggtacagg cacgccatta
    38581 caagtcagta cataacgatc actcggcagg ccgctgagct tgacctgcat gcgttccagc
    38641 gaggaatcga catagcgcac cgtaccgccg ccaccgccct cctcgcccag tacatgccag
    38701 ggctccaggg cctgacgcag ttccagctcg atgcccttga cgttgaaatc gccggatttg
    38761 ggaaaacgga actcgaaatg cggggcaaac cattcgctgt gcaggcggta gccgcagccg
    38821 tccagctcct gcaatacgtc ggcaaagtcc tgctcgataa aatgcggcag caggtagcgg
    38881 tcatgcagct cggtgcccca acgcaccagt cgcgccggtt tgtagggctc gcgccagaaa
    38941 cgcgcgatca gcgcgcgaat caacagctgc tgggccaggc tcatctgcgc atggggcggc
    39001 atctcgaagg cgcgcagctc cagcaggccg aggcgcccgg aggccgagtc cggcgaatac
    39061 agtttgtcga tgcagaactc ggagcgatgg gtgttaccag tcacgtccac cagcaggttg
    39121 cgcagcaaac ggtcgaccag ccagggctgg cagtcgcggc ccacttcggg catctgcgcg
    39181 aaggcgattt ccagctcgta cagcgcatcg ttacgcgctt catcgatacg cggcgcctgg
    39241 gacgtcggcc cgataaacag cccactgaac aggtaggaca gcgacgggtg gttgtgccag
    39301 tagctgatca ggctgcgcag cagatccggc cgtcgcagaa acggcgagtc cgcaggcgtc
    39361 gcaccgccga ggacaaagtg gttaccgccg cccgtaccgg tgtggcggcc gtcgatcata
    39421 aatttctcgc tggagaggcg gcactcacgg gcctgctcgt agagaaattc ggtgcgttcg
    39481 accagctcat cccagctagc agccgggtga atattcacct cgatcacgcc ggggtccgga
    39541 gtcactcgga agaactgcag acgcgggtcc agcggcggct cgtaaccttc cagcagcacc
    39601 gggcatttca gctcggcggc agtggcttcg atggcggcca ccagctccag atagtcctcc
    39661 agacgcacca gcggcggcat aaacagatag agcttgccgt cgcgcggttc ggcgcacagg
    39721 gcggtacgca ctacaccagc cgctgattgg cccagcaacg gacgcttgtt tacaccgctc
    39781 tggctgcccg accagacacc acgcgactgc ggcttgatct gcggcgcagc cggcagtggc
    39841 gcaaatacct gacttgggtc ttccgggttt acgtaggggt aatcgccctc gctgacccaa
    39901 ggttgcgaat ccagcggcag gcggtagccc atcgccgagt cgccggggat caggcggcaa
    39961 tgctcatcac gcagaaacca gctgccactc tgccagccca actgcttggc atgtcgggcc
    40021 agcggcagga tatgcccgac cactttatcc aggccctgct cgaatacctt gcgcaggcgc
    40081 tcacgctcca gcgcatcggc tagacgtgaa tcttcagcgg tgacgttgct cggcagcttg
    40141 cgttcacgcc acaggtagta gaagcggtct tcataggcag gcacgcagtt cttgccgcct
    40201 acacccaggc gctcggcgag tagggtaaga aagtcggcgg ccttggccgc atcgacgccg
    40261 tagtcctgct gctcgtcggc atacagcgcc gggttttgcc agatcggttc accgtcacgg
    40321 cgccagtagg cattcagcga ccagcgtggc aactgttcgc cggggtacca cttaccctgg
    40381 ccgaaatgca ccagcgcctt ggacgcgtag tgctcccgca gtcggtggaa taaatcagtg
    40441 gccaggcggc gcttgttcgg ccccagggcg gcggtattcc attccgcatc gtcggggtaa
    40501 tcgagggcga caaaggtcgg ctcgccgccc atggtcagac gtacgtcctg ggcctgcagg
    40561 tcctcgtcga tcttctgccc gagggcaaca atcgcctgcc actgttcatc gctgtaaggc
    40621 ttgctgactc gcggcgcttc ccagatacgc tcgacccaca tttcatggct gaactcgcac
    40681 tcgctctcat ccacgccgcc gctgatcggc gccgccgagg acggttcggg gctgcaagcc
    40741 agcggaatat gaccttctcc ggcgaacagg ccgctggtcg ggtccaggcc aatccatccg
    40801 gcgccgggca ggtagacctc gcaccaggca tgcagatcgg tgaaatccac ctcggtgccg
    40861 gatgggccgt cgagggattt catatccgct ttcagctgaa tcaggtagcc ggaaacaaag
    40921 cgcgccgcca ggcccaggtg gcggaacagc tgcaccagca gccaggccga atcgcggcag
    40981 gagccggagc ctttctgcag gctttcctcc ggggtttgca cccccggctc caggcggatc
    41041 aggtaactga tatcggtggc caggcgttgg tttagcgcca ccagaaaatc cacgctcggc
    41101 agtggcgtgc gttcgatgct ggccaggtac tcagagaaca atttgccggc cggcagcttg
    41161 actagataag gcgccagctc cagctgctcg ccctcggtat aggcgaaggg gattttctcg
    41221 gcgtaaggct caaggaagaa atcgaagggg ttgaacaccg ccatttcggc gaccagatcg
    41281 acctcgatct tcagctcgcg ggtcttttcc ggaaacacca ggcgcgccag gtagttgccc
    41341 tgggggtcct gctgccagtt gataaagtgc ttgcccggct cgaccttcaa ggcgtaggac
    41401 aggacccggg tacggctgtg cggtgcaggg cgcaggcgca cgacctgcgg gccaaggttg
    41461 acggcgcggt cgtagcggta gtggctaaca tggtgcaagg cgatatgaat cgacacagtg
    41521 ggcctcctgc aagcctgggc gataagccct tcagcgcaag acttatgcca gtggctcagg
    41581 ccaggtgctg cggggcttgc cgcgccccca tgtcacgcag gcgcaccata acgaggccag
    41641 gcctgcccta tgcctgccgc ggcgcacgaa aataggtcgc aaccctcaac aatccccttg
    41701 caggctggcc acccagcgct gcagcacctc gaccccttcg gtatgcacgg tggagcggcc
    41761 cagctccggc atcatcatgc tcgggtcgac gctctccacg cgaaacagca gaaccgactt
    41821 gtccggctgc ccagggtgaa tatccaccag acgatcaccg gaacctttgc cggccgccac
    41881 cggctgcttg cacaggccat agctgatgcc gagggtcgtg gccggatcga gcaacagccc
    41941 cgaggtacgc cccggtcccg tggggttgtg gcaatggctg cagttggcat ccagataact
    42001 gcgcgcctgc tgttccaggc tttcgccact gcgctgggtc ggccataaag cgttctgcgg
    42061 cacgccctgc aactcagccg gcacgccctg cagacggccc agctgctgcc agtgctgcaa
    42121 ttggttgtgg ctgccatcgg cgtaggcaaa atccttgttc aggtgtcttg ccttggggcc
    42181 cagtggattc acgccctgcc ctgcagtttc ctcatgacaa ccggcgcact ggttggcatc
    42241 cggcacctga taggccaccg cctgagtttt gccctcggca tccaacaggc tcaactggcg
    42301 gctgtcgccc gcccattcca gatgcgcctc ctgttgctgt tcatcccaca catagggcaa
    42361 cgccacccaa ccgtcctgct gtttaagtag gatgcgcgtt tcgattaact gtacccgcgc
    42421 cagctccagc ccctgttgcg ggttgcggtc atcctgctca ttaagcaaca ggcgaccctg
    42481 gccgtccttg ggatagtaga aggtcttgct cagcaccgtg ccaaccgggt aatcgaaatg
    42541 cgcctcgcca tagttcgccg ctgttcccgt cggcatccac acggtgcgca gcttgtgtgc
    42601 atagtcagtg aacaatggag tattcaggtc atagggcagc gcttctggca cttgcgcgat
    42661 aaagccatcg gcgcgttgca gcatgcccca ttcactgagc ttctcggggt agttgtcacc
    42721 ttgcggcagg tacagcggcg ccggctgctg ctcgcagcca gcgagcagca gcagcgcccc
    42781 cagtaacaga cgacgcatca agccccctgg tcggcgtcag ccgaggccag tttgaccgct
    42841 ggcagcgcgg gcaggctgca acggtagtcg gccatttcgg tactgatgtt cttgtagttg
    42901 ttcggcccgt cgacgttgat catccccgcc tcaccgttat tgatgcagat cgcccgatcc
    42961 gccggcagct tgccatccac taggatgtcg aggttgacgt agccatccca gagaatgtcc
    43021 ggcaggcggc cgttcaagcc gaactggctg accttcagcg ctttcagctc caggtgatcc
    43081 gggctatcgc cacctgggcc gaagcggttg ccgtgaatat ggatgccttc tggataggga
    43141 tcgaagttct cggcggtcga gaggtcggtg taaccggtgc tgaagtagct gctgataatt
    43201 atgttggcgg tcttgtggtc gccgatatcg ttgtcgaaca cctcgacatc gtcattggag
    43261 ttgatcacca ccccagagcc ggccggcacg ctggccaccg gtgtgccttt gtggccgaag
    43321 ttcttgtggt tgttgccttc gatcttgttg cggaacaccc gcgtcacccg cccggcctgc
    43381 ggcagattgg gcatgttgaa caccagaatg ccgccagtgt tgccggtggc gacgttgtca
    43441 tacacatcgg cgtcgatggt gttttcgatt tcgatgccgg cgacgttgcg ctcggcgcgg
    43501 ctgttgcgta ccaccacatt gcgcgactgg ccgacataga tgccggcgtc cgaggcgccg
    43561 atggccaccg cgccgtcgat cagggtgttc tcggtctgca ccgggtagat gccgtaggcg
    43621 ccgttctcgg tggccgggcc gttggtccac tcggtgcgca cgcggcggat gatgatgttc
    43681 ttgccgccca ccactttcag cgaatcacct agggtgtctt caaaggcgat gtcttcaata
    43741 gtgaaatccg aggcatccac caacaggcct tcggcgccgg acttctgccc cttgaagctg
    43801 aggatggtct tgtccattcc cgcacccttg atggtcacgc cactgacttt caggctcagg
    43861 ctgcggtcga gctggtaggt gccggcagga atctcgatca cactgccagg cttcaccttg
    43921 atcagctggg tctgcaggtc tttctggaaa tccagatcag ccaccggggc cggttcttcg
    43981 ctgcaggcgg caagcagcac gaacaggaca agcacggaaa gcagggcaag gcgcatggca
    44041 atctccagcg tttttcttat taattggaac gatagtacca attaataaga aaaagccaag
    44101 ccccgcgcca gcacagcgca actctcccga ccaacagcca gcctacgcag agcgcgacgc
    44161 atccgttacc atcgccggca attaagcgaa gagatgcggc atgtatatct atcgattggt
    44221 gctgatcctg gtggtgggga tctacctgtt ctccccggcc atcatggact ggtggatcga
    44281 ccccaatggg gcctggtacc gcccctacct gctgtggctg atcctgatag tcgtgacctt
    44341 cattcttcag agccaacgtg atgctgacga gctttagcct gagccagctg attctgatca
    44401 gcgctgccta ccttcttatt ctgttcggcg tggcctggat cagtgaacac ggcctgattc
    44461 cacgctgggt gattcgtcac ccgctgacct acaccctgtc gctcggcgtc tatgccagtg
    44521 cctgggcctt ctacggcacg gtgggtttgg cctatcaata tggctatggc ttcctcgcca
    44581 gttatctggg ggtgtccggg gcctttttgc tagcgccggt gctgctctac ccaattctgc
    44641 gcatcacccg cacctaccag ctgtcatcgc tggccgacct gtttgccttc cgctttcgca
    44701 gcacctgggc cggcgcgcta accacggtgt ttatgctgat cggcgtactg ccgctgctgg
    44761 ccctgcagat tcaggcggtg gccgattcca tcggcatcct cacccgcgag ccggtgcagg
    44821 agcgcgtggc gttagcctat tgcgggctaa tcattctgtt caccatcctg ttcggcgctc
    44881 ggcatatcgc aacccgcgag aagcatgaag gcctggtgtt cgccatcgcc ttcgagtcgg
    44941 tggtcaagct ggtggcgatc agcgccatcg gtctgtatgc gctgtacggc gtgttcgacg
    45001 gcccgcagga cctgcaatcc tggctggtgc aaaaccagtc ctcgctgacc agcctgcaca
    45061 ccccgctgca ggaaggccca tggcgcaccc tgctgctggt gttcttcgcc tcagcgatcg
    45121 tcatgccgca catgtaccac atgaccttta ccgaaaacct caacccacgc gccatggtca
    45181 gcgccagctg gggcctgccg ctgttcctgc tgctaatgag tttggcggtg ccgctgattc
    45241 tctgggccgg gctcaagctg ggcgccacca ccaacccgga atatttcacc cttggcgtgg
    45301 ggatcgcggt gaatagccag accctggcgt tgatcgccta cgtcggcggc ctgtcagcct
    45361 ccagcgggct gatcatcgtc tcgaccctgg cgctgtcggg catggtgctc aaccatgtag
    45421 tgctgccgct gtaccagccg ccatcggaag gcaatatcta ccgctggctg aagtggacgc
    45481 gccgtgcact gatcctgctg gttatcatgg ccggttatgg cttttattta ttgctgggcg
    45541 ccgaacagga cctggccaac ctgggcatcg tcgccttcgt cgccaccctg cagttcctcc
    45601 ccggcgtgct ctcggtgctg tactggccga ccgccaaccg caaaggcttt attgccggct
    45661 tgatggcggg gatcggcatg tgggtggtga gcatgctgtt gccgatgctc ggcaacctgc
    45721 agggtctcta cctgccctgg ttcaacgtca tctatgtatt ggatgacagc agttggcacc
    45781 tggcggccat cggctcgctg gccgccaacg tgctggtatt caccctggtg tcgctgttta
    45841 ccgaagccag cccagaagaa aaaagcgccg ccgaagcctg cgccgtggat aacgtgcgcc
    45901 gcccgcagcg ccgtgaactg ctggccgcct caccgcagga gtttgccgcg caattggcca
    45961 aacctctggg cgccaagacc gcccaacatg aagtggagca ggccctgcgc gatttgcacc
    46021 tgccgttcga tgaaggccgc ccctatgccc tgcgccgcct gcgtgaccgc atcgaggcca
    46081 acctctccgg gctgatgggc cccagcgtgg cccaggacat cgtcgagaca ttcctgcctt
    46141 ataaggccgg cagcgaaacc tacgtcaccg aggacatcaa ctttatcgag aaccgcctgg
    46201 aggattacca ctcacgcctc accggcctgg cggccgaact cgacaccctg cgccgctatc
    46261 accgacagac cctgcaggag ctgccgatgg gcgtctgctc actggccaag gatcaggaga
    46321 tcctgatgtg gaacaaggcc atggaagaac tcaccgaaat ccccgcgcag cgggtggtcg
    46381 gctcgcgcct gaacaccctc ggcgaaccct ggcgcgggct gctgcagaac tttatcgaat
    46441 tgcccgacga gcacttacac aaacagcgcc tggccctcga cggccagacc cgctggctca
    46501 atctacacaa ggcggccatt gccgagcccc tggcgccggg taacagcggc ctggtattgc
    46561 tggttgaaga cctcaccgaa acgcagatgc ttgaagacaa gctggtgcac tccgagcgcc
    46621 tggcctccat cggtcgcctg gccgccgggg tggctcacga gatcggcaac ccgatcaccg
    46681 gcatcgcctg cctggcgcag aacctgcgcg aagagcgcga aggcgacagc gagctgaccg
    46741 agatcagcga gcagatcatc gagcagacca agcgcgtgtc gcgcatcgtc cagtcactga
    46801 tgagctttgc ccattccggc agccaccaga acagcgagga gccggtgtgt ctggctcagg
    46861 tcgcccaaga cgccatcgcc ctactggccc tcaaccggcg cagcgtcgaa gtgcagtttt
    46921 tcaacctctg cgattccgac cactgggtgg tcggcgatcc gcaacgcctg gcccaggtgc
    46981 tgatcaacct gctgtccaac gcccgcgatg cctcaccgcc gggcagcgcg atccgggtca
    47041 agagcgaacg tttcgagcac accatcgacc tgatcgtcga ggacgaaggc agcggtattc
    47101 ccaaggcgat catcgaccgc ctgtttgaac ccttcttcac caccaaggac ccaggcaaag
    47161 gtacggggct gggccttgca ctggtctatt cgatcgtgga agagcattat ggacagataa
    47221 caatcgacag cccggccgac cccgagcttc agcgcggcac ccgcattagg gtcagcctgc
    47281 cgaggcatgt cgaggcgacg tccatagcga cttaaaccag ctaccagcga gcatgcctgc
    47341 caggcccctt ggtccagcgg catgccccga gaccgtcgag agactgaatt gatgcctcat
    47401 attttgatcg tcgaagacga aaccattatc cgctctgcct tgcgtcgcct gctggaacgt
    47461 aaccaatacg aagtcagcga agccggctcg gtgcaggaag cgcaagagcg cttcagcatc
    47521 cccagtttcg acctgatcgt cagcgaccta cgcctaccgg gcgcgcccgg caccgagctg
    47581 atcaagctcg gccaaggcac cccggtgctg atcatgacta gctacgccag cctacgttcg
    47641 gcggtcgatt cgatgaagat gggcgcggtc gattacatcg ccaaaccctt cgaccacgat
    47701 gaaatgctcc aggccgtggc gcgtatcctg cgcgatcacc agcaggccaa gagcgcaccg
    47761 gtacccgcag ccagcacggc gaaagtcagc agcgacaagg tggtggacaa cagtaacgga
    47821 gaaatcggca tcatcggttc ctgcgcagcc atgcaggaga tgtacagcaa gatccgcaag
    47881 gtcgcgccca ccgactctaa cgtgctaatc caaggcgagt ccggcaccgg taaagagctg
    47941 gtggcccgcg ccctgcacaa cctctcgcgc cgcgccaaag caccgctgat ttcggtgaac
    48001 tgcgccgcca tcccagaatc gctcatcgaa tccgaactgt tcggccatga gaaaggcgcc
    48061 tttaccggcg ccagcgctgg ccgtgccggc ctggttgaag cagctgacgg cggcaccttg
    48121 ttcctcgatg aaatcggtga actccccttg gaagcccagg cgcgtctgct gcgtgtactg
    48181 caggaaggcg agattcgccg ggtcggctcg gtgcagtcac agaaagtcga tgtacgcctg
    48241 atcgccgcga ctcaccgcga cctgaaaagt ctgtcaaaga tcggccaatt ccgtgaagac
    48301 ctgtattacc gcctgcacgt aatcgccctg aaactaccgc cgctgcgtga gcgcgggacg
    48361 gacgtaatcg atatcgctcg tgccttcctg attcgtcaga gcactcgcca gggccgtctc
    48421 gacctgagct ttgccgctga tgccgaacaa gccattcgtc attacccctg gccgggtaac
    48481 gtgcgcgaac tggagaacgc catcgagcgt tcggtgattc tctgcgaagg cagtagcatt
    48541 tccgctgagc tgctgggcat cgatatcgag ctggatgacc tggaagacga ctttagcggc
    48601 ctgcccgcgc aaagcggcgg cctgggacat gaaccgagcg aaccgaccga agacctgtca
    48661 ctggaagatt acttccagca cttcgttctt gagcatcagg accacatgac cgagaccgaa
    48721 ctggcacgca aactcggcat cagccgcaaa tgtttgtggg aacgccgcca gcgtctgggc
    48781 attccacggc gcaaatcctc gggcgcaacc gcaggctaga gcggattgcc aaggtgacag
    48841 atgcttacgc aagaggttcc ctcatctgtt acccattcaa cacctagtaa caaaagccgg
    48901 gtcttacagt aacgagaacc cggctttttt atgccacctc agcgcccact gcacagctca
    48961 acaccttgtt cttaaaggga ttacaaaaac tggcacgggt tctgctttat tgctggcaca
    49021 acaacaataa caagcaagct ccaaccctaa taaaaataag acgtaccgac tccagcacaa
    49081 taaaaacaac aacgcggagg cgcagctaac tgattctttt ggaaaggaat tgcctttggg
    49141 gttcacccca aaaccaggct gagaattata aaactgcctc gaggcagcgc cagaactggt
    49201 tgggtcactg tgtgatcatg gcagcgtcag catccaaagc aatccgtttg ctcttgattc
    49261 ccagcttggg aaagtttcca aaagtctttg actggatgga acgggcaaat caacaaaaac
    49321 aacaagcccg ccataataat aaaaacagag cacgcaccaa tttggggggg agcttcggct
    49381 cccccagtag cttcaaatcc cccgcctgcc gccctttcct gtcgtttcct gcatcaaacc
    49441 tcgactcaat gctagaatcc gcgccattcc tgcggccggc atatactctg gccagtcatt
    49501 tagtcaaaca gtgcctccca tgctgaaaaa gctgttcaag tcctttcgta cccccatccg
    49561 tcgtggcaac cattcgcaca gcacgcccga agtgctgagc agtagccagc acccgatcga
    49621 gcgcggtgag atcagccgct acgccatcag cgtggtcgaa cgcctgcaac aggccggcta
    49681 tcaggcgtat ctggtcggtg gttgcgtgcg cgatctgctg ctggatatcg accccaagga
    49741 tttcgacgtg gccaccagcg ccacaccgga acaggtgcgt gccgagttcc gtaacgcccg
    49801 ggtaatcggt cgacgcttca agctggtcca cgtgcatttc ggccgcgaga tcatcgaagt
    49861 cgccaccttc cgcgccaatc atcccgaagg cgatgaggaa gagaacagca acctgtcctc
    49921 gcgcaacgag agcgggcgca tcctgcgtga caacgtgtat ggcagcctgg aagacgacgc
    49981 ccagcgccgc gacttcacca tcaatgcgct gtattacgat ccaagcaccg aacgcattct
    50041 cgattacgcc aatggcgtgc acgatatccg caatcatctg atccgcctga tcggcgaccc
    50101 gacccagcgt taccaggaag acccggtgcg catgctgcgc gcagtgcgtt ttgccgccaa
    50161 gctggatttc gacatcgaga aacacagcgc cgcgccgatc tacaaactgg ctccgctgct
    50221 cggtggcatc cccgccgcac gcctgttcga tgaagtgctc aagctgtttc tcgctggcta
    50281 cgcggtgtac acctacgagc tactggtgga ttacggcctg ttcggccagc tgttcccggc
    50341 cagcgccgaa gcgctgaaac tcaacccgga ctacaccgac cagttgatcc gtaatgcgct
    50401 ggacagtacc gatctacgca ttcaacaagg caagacggtt actccagcct tcctgcttgc
    50461 tgcactgctg tggccagccc tgccggcccg cgtgatcaag ctgcaggatc gcggcatgcc
    50521 gccgatcccg gcgatgcagg aagccgccca tgagctgatc agcgaacagt gcctgcgcat
    50581 cgctgtgccc aaacgtttca ccctgccgat ccgcgagatc tgggacatgc aggagcgcct
    50641 gccacggcgc agcggcaaac gtgccgacct gctgctggac aatctgcgat tccgcgccgg
    50701 ctacgacttc ctgctactac gcgaaagcgc gggcgagcag accgatggcc tgggcgaatg
    50761 gtggacggac taccaggacg ccaacgacag tgagcggcgc gacatgattc gcgacctcag
    50821 tggcaaggac gacggcaccg gcggcccacg caaacgtcgt cgcagcggcg gcgccaagcg
    50881 caagcgttca gcagacgctt cgagcaccac gggcgagtaa tccatggaac gcatctacat
    50941 cggcatgggc agcaacctcg ttgaccccgc cgaacagctg cgcagcgcca ccctggcgct
    51001 ggcacagttg ccgcaatcgg aactggtcgg ggtttcagcc ttctatcaaa gcgattcgtt
    51061 gctccctggc caaccgcgct acaccaacgc ggttgccgct ctcgacagca ctctggcacc
    51121 gcttgagctg ctggacgcct tgcaatccat tgaaaacgac caaggccgcg agcgccttga
    51181 gcgctggggg ccgcgcaccc tggatctcga catcctgctg ttcgg
//
LOCUS       DASRUM010000091         9468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_214598, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000091 DASRUM010000000
VERSION     DASRUM010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9468
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214598"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /gene="gyrB"
                     /locus_tag="VFF22_06250"
     CDS             <1..412
                     /gene="gyrB"
                     /locus_tag="VFF22_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551319.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HZX16451.1"
                     /translation="HGVSSYIIFNRDFFASNDYKTVTTLGDQLNSLLEEGAFVQRGER
                     KKHVSTFKDALAWLMAESTKRHSIQRYKGLGEMNPDQLWETTMDPEVRRMLKVRIEDA
                     IAADQIFNTLMGDAVEPRRDFIETNALAVSNLDF"
     gene            complement(480..1421)
                     /locus_tag="VFF22_06255"
     CDS             complement(480..1421)
                     /locus_tag="VFF22_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZX16452.1"
                     /translation="MDNNTLLLFAGIGALLLLASAIGYFLKWRNGDSANPVIDNLNAR
                     INAWWVMVLVIGIAFLFGNLGVILLFYFVSFYALREFMTLTPTRRSDYPALVAAFYFA
                     LPMQYLLIALDWYGLFAIFIPVYLFLLLPILASLGGDTTRFLERASKVQWGLMIAVYC
                     ISSVPALLTLDIPGYEGRNLLLIAWLIIVVQLSDVLQYVCGKLFGKHKIAPRLSPSKT
                     VEGFVGGVSLSCLVGALLCWITPFNFWQAALMALTVNLLGFAGGLVMSAIKRDRGVKD
                     WGHMIEGHGGMLDRLDSVCFAAPIFFHFVRYWWVPLA"
     gene            complement(1423..2043)
                     /locus_tag="VFF22_06260"
     CDS             complement(1423..2043)
                     /locus_tag="VFF22_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008094198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZX16453.1"
                     /translation="MLASLIAFVITSAARLLTGARALWLGCTPQPVQRLYYANHSSHG
                     DFVLLWASLPDELRRRTRPVAGADYWQRDGLRRYLINKVFNGVLIDRERATPEANPLQ
                     PMLDALDQGDSLILFPEGTRNLEDGLLPFKSGLYHLTRLRPDIELVPVWIANLNRVMP
                     KGRALPLPLLCTLSFGTPLEHLHDENKQAFLERARNALQALAPEEA"
     gene            complement(2054..2509)
                     /locus_tag="VFF22_06265"
     CDS             complement(2054..2509)
                     /locus_tag="VFF22_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008073631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16454.1"
                     /translation="MATEMQLFTVASLLRRGRALDQLSNALSLVALLIGLAPLLGIKA
                     QPLTALFCALLLILGLGQKYWALRVALDAELFQQLASRVDQLAAHTAELDQTLVQLKL
                     QPANLPARGWDERSLGALRLLRQQVLWLVAQLLLAVLIVLGLPWLAITG"
     gene            complement(2499..3854)
                     /locus_tag="VFF22_06270"
     CDS             complement(2499..3854)
                     /locus_tag="VFF22_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HZX16455.1"
                     /translation="MAEVSLSREAGLWKRGVLWLLLLAPLFFASYSFANWLSAQRDDV
                     GTLVFAWESHMPLWPWTIAPYWSIDLLYGLSFLLPATRREMDRHGLRLLSVQVLCIAC
                     FLLWPLRFTFERPELDGLFGGMFDVLMGFDKPFNQAPSLHIALLVVIWVMFARHTHGL
                     WLRGLLHGWMALIGVSVLTTWQHHFIDVPTGILAGWLCVWLWPLQGRSPLLAWQLTRE
                     PQRMRLALRYSLGAALLSALAVNLGGAWLWLLWPAFSLLLVALNYLLFGAVGFQKTAD
                     GRLSSAASWLLAPYLGAAWLNSRLWTRAHPHPDEVEPGIWLGRIPAPGQAEQFQAQLD
                     LCAELPASAPAKAYRSLPLLDLCTPSPAACLEAAQAIEALRTHGNLLVYCALGYSRSA
                     TAVAAWLLHSGRCKDVPEALAHLRRARPQVVLSDAHQQALQGMLELSNSSMPAGAAHG
                     Y"
     gene            complement(3854..5611)
                     /locus_tag="VFF22_06275"
     CDS             complement(3854..5611)
                     /locus_tag="VFF22_06275"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HZX16456.1"
                     /translation="MREAQEFSFTTHDGVELFYRHWPASNTDGAAPKRAVVMFHRGHE
                     HGGRMAHLVDELEMADFDFFAWDARGHGLSPGERGDSPSFATSVRDVQTFIDHIVSHH
                     GLAVEDIAVLAQSVGAVVVATWAHDYAPKIRALVLASPAFKVKLYVPFARPGLKLMRA
                     LRGNFFVNSYVKAKYLSHDPERIASFINDPLIARPISVNMLLGLYNAAERVVADAQAI
                     QIPTQLLISGADFVVHRKPQQQFFERLGSLRKEMHILPGFFHDTLGERDRSHALKRAQ
                     RFLLRCFEEPVARPSLLDADRLGVTCAESESLAAPLPHNSLGDLYWRATRAGLRLGSS
                     LADGVKLGFDTGFDSGSTLDYVYRNQPSGKSPIGRLIDQNYLNSIGWRGIRQRKLHAE
                     ELLRLAMGKLREQGRGVRIVDIAAGHGRYILEALEGMEQKPDSILLRDYSDINVRDGG
                     VLIEQKGLSDIASFVKGDAFDRADLAALEPKPTLAVVSGLYELFGSNQMVGESLSGLA
                     AAVEEGGYLVYTGQPWHPQLELIARALTSHRGGEAWVMRRRSQAEMDQLVEAAGFRKI
                     TQRIDEWGIFSVSLAQRVS"
     gene            complement(5622..6245)
                     /locus_tag="VFF22_06280"
     CDS             complement(5622..6245)
                     /locus_tag="VFF22_06280"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016703978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZX16457.1"
                     /translation="MLSIYQLKPAFQNLLRPGVKRLYDKGVTANQVTLFAAVVSVLLG
                     TLLLALPQHTWLFALIPLWMILRMALNAVDGMLAREFGQQSRLGAYLNELSDLIADSA
                     LFLPFALLPGVSPLLVVLVVLLALISEYAGVLGPMVGASRRYDGPMGKSDRAFCFGVL
                     GAGVACGLLSAAWLNGLLAVILALLLYTLYNRVRQGLAEAAQPTDSK"
     gene            complement(6493..7266)
                     /locus_tag="VFF22_06285"
     CDS             complement(6493..7266)
                     /locus_tag="VFF22_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZX16458.1"
                     /translation="MSTVQTLRTFLFYLLLASTSFFWCTLSVFVAPFLPFRARYRFVV
                     QNWCRCATWLAKVVVGINYEIKGLENIPDKPCVILAKHQSTWETFFLCGYFEPLSQVV
                     KRELLYVPFFGWAMAMLKPIAIDRSNPKAALKQLAKLGHKRLGQGAWVLVFPEGTRIP
                     PGQIGKFSRGGASLAVNANLPVLPIAHNAGEFWPKQGWRKTPGTVQVIIGPPMYAEGT
                     GPRAIAELNDRAFAWVSQAQRDIGSLDAQSQPLETQAPA"
     gene            complement(7299..7832)
                     /gene="gmhB"
                     /locus_tag="VFF22_06290"
     CDS             complement(7299..7832)
                     /gene="gmhB"
                     /locus_tag="VFF22_06290"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003220221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HZX16459.1"
                     /translation="MKLLILDRDGVINYDSDAYIKSLAEWIPLPGAIEAIARLSQAGW
                     TVAVATNQSGVARGYYDLATLEAMHARLRELVAAQGGELGLIVHCIHGPDEGCACRKP
                     KPGMLEQIAAYYGVALKGVWFVGDSSGDLTAAGAVDCQPVLVKTGKGERTLAKGLPAG
                     TLVFDDLAAVADQLLHL"
     gene            complement(7923..>9468)
                     /gene="glyS"
                     /locus_tag="VFF22_06295"
     CDS             complement(7923..>9468)
                     /gene="glyS"
                     /locus_tag="VFF22_06295"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_064728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HZX16460.1"
                     /translation="CEILAQQAGRVSRGHRFHANHEVRISAPANYAEDLRSAYVIADF
                     AERRAQIAARVDQLAAEQQGTAIVPPALLDEVSALVEWPVPLVCSFEERFLEVPQEAL
                     ITTMQDNQKYFCLLDGNGKLLPRFITVANVESKDPAQIISGNEKVVRPRLTDAEFFFK
                     QDKKQKLESFNQRLANVVFQAQLGSVYDKAQRVSALAGFIAQRIGGDATRAARAGILC
                     KCDLASEMVGEFPEMQGIAGYYYAKHDGEAEDVALALNEQYMPRGAGAELPSTLTGAA
                     LALADKLDTLVGIFGIGMLPTGSKDPYALRRAALGVLRILIEKQLDLDLVEAVSFAIG
                     QFGDKIKADGLAPQVQDFIFDRLRARYEDEGVEVAVYQAVRAVSPTSPLDFDQRVQAV
                     QAFRALPQAAALAAANKRVSNLLSKAEGQVAASVEAHHFDNPSEFALNAAIQQAEHAV
                     QPLAAARQYNLALSQLANLREPVDAFFEAVLVNAEDPAVRANRYALLAKLRGLFLGVA
                     DISVLG"
BASE COUNT         1868 a         2897 c         3093 g         1610 t
ORIGIN      
        1 ccatggggtt tccagctaca tcatcttcaa ccgcgacttc ttcgccagta acgactacaa
       61 gaccgttacc accctgggcg atcagctcaa cagcctgctg gaagaaggtg cctttgtgca
      121 gcgcggcgag cgcaagaagc acgtcagcac cttcaaggat gcactggcct ggctgatggc
      181 cgaaagcacc aagcgccata gcatccaacg ctataagggt ctcggcgaaa tgaacccgga
      241 tcagctctgg gaaaccacca tggacccgga agtacgccgc atgctcaagg tgcgtatcga
      301 agacgcgatc gctgccgatc agatcttcaa caccctgatg ggcgatgccg ttgagccgcg
      361 tcgggacttt atcgagacca acgccctggc agtgtccaac ctggacttct gatccgctgc
      421 agctcgtatc agccacaaaa aatgccccgg atatccgggg catttttatt ccgctcgact
      481 caagccaacg gcacccacca gtagcgcacg aagtggaaga agatcggcgc ggcgaaacac
      541 actgagtcga ggcggtcgag catgccgccg tggccttcga tcatatggcc ccagtccttt
      601 actccgcggt cgcgtttgat cgccgacatc accaggccgc cggcaaagcc cagcaggttg
      661 acggtcagcg ccatcaaggc ggcctgccag aagttgaacg gggtaatcca gcacagcagc
      721 gcgccgacca ggcaggacag gctgacgccg ccgacaaagc cctcgacggt tttcgacggt
      781 gacagacgcg gcgcgatctt gtgcttgccg aacagtttgc cgcagacgta ctgcaacaca
      841 tcggacagct gcaccacgat gatcagccag gcgatcagca gcaggttgcg cccttcatag
      901 ccggggatgt ccagggtcag cagcgcgggt accgaggaga tgcagtacac cgcgatcatc
      961 aggccccact gcacctttga cgcacgctcg agaaagcgcg tggtatcgcc gcccagggac
     1021 gccaggatcg gcagcagcag gaacagatac accgggataa agatggcgaa caggccgtac
     1081 cagtcgaggg cgatcagcag gtactgcatg ggtagggcga agtagaaggc cgccactaat
     1141 gccgggtagt cgctgcgtcg ggtgggcgtg agggtcataa actcgcgcag ggcgtagaac
     1201 gagacgaagt agaacagcag aatcacgccc aggttgccga acaggaaggc gatgccgatc
     1261 accagcacca tgacccacca ggcgttgatc cgcgcattga ggttgtcgat taccgggttg
     1321 gcgctgtcgc cattgcgcca cttgaggaaa tagccgatgg ccgaggcgag cagcaacagc
     1381 gcgccgatac cggcgaacag cagcagggtg ttgttatcca tatcaagcct cctcgggtgc
     1441 tagggcctgc agggcgttac gggcgcgttc gaggaacgcc tgcttgtttt catcgtgcag
     1501 atgttccagc ggcgtgccga aactcagggt gcacagcagc ggcaacggca acgccctgcc
     1561 cttgggcatg acccggttga ggttggcgat ccacaccggt accagttcga tgtcgggacg
     1621 cagccgggtg aggtgataca gaccgctctt gaacggcaac aggccgtcct ccagattgcg
     1681 cgtgccctcg ggaaagagga tcaacgagtc gccttgatcc agggcatcaa gcatcggctg
     1741 cagcgggttg gcctcaggtg tggcgcgctc gcggtcgatc agtacgccgt tgaatacctt
     1801 gttgatcagg tagcgacgca ggccgtcacg ctgccagtaa tcggcgccgg ccacgggccg
     1861 ggtgcggcgg cgcagttcat cgggcagcga tgcccagagc agcacgaaat cgccgtggct
     1921 gctgtggttg gcgtaataca gccgctgcac gggttgtggc gtgcaaccca gccacagcgc
     1981 gcgggcaccg gtgagcaggc gcgcagccga ggtgataacg aacgcgatga gggacgcgag
     2041 catggacgac tccttatcca gtgattgcaa gccagggcag gcccagcacg atcaacacag
     2101 ccaacagcag ttgcgccact agccagagca cttgctggcg caacaggcgc aacgcaccga
     2161 ggctgcgttc atcccagccg cgcgcgggca gattagccgg ctgcagtttg agctggacaa
     2221 gggtctggtc gagctcggcg gtgtgcgcgg ctaactgatc aacccggctg gcaagttgtt
     2281 ggaacagctc ggcatccaag gccacgcgca gcgcccagta tttctgcccc agacccagta
     2341 tcagcagcag cgcgcagaac agcgccgtca gtggctgtgc tttgatgccg agtagcggtg
     2401 ccagtccgat cagcagggca accaggctga gcgcgttgga cagctgatcc agcgccctac
     2461 cccgacgcag cagactggcc acggtaaata gctgcatttc agtagccatg ggcggctccc
     2521 gctggcatgg agctgttgct gagttccagc atgccctgca gcgcctgctg atgagcgtcg
     2581 ctcaagacca cctgaggccg cgcccggcga agatgcgcca gcgcttctgg cacatctttg
     2641 caacgcccgc tgtgcagcag ccaggccgct accgccgtgg cgctacgcga atagcccagg
     2701 gcgcaataga ccagcaggtt gccgtgggtg cgcagcgcct cgatggcctg cgcggcttcc
     2761 aggcaggctg ccgggctggg agtgcagagg tcgagcaatg gcagtgagcg ataggccttg
     2821 gctggggcgc tggccggcag ctcggcgcat agatcgagct gagcctgaaa ctgctcggcc
     2881 tgcccgggcg cgggaatgcg ccccagccaa atgcccggct cgacctcatc agggtgcgga
     2941 tgggcgcggg tccaaagccg tgagttaagc caggcggcgc ccaggtaggg cgcgagcaac
     3001 cagcttgcgg cgctgctcaa tcgaccatcg gcggtctttt gaaagcctac cgcgccgaac
     3061 agcaggtagt tgagcgccac cagcagtaag gagaatgccg gccataacag ccacaaccag
     3121 gcgccgccga ggtttactgc cagggcgctg agcagcgccg cacccaggct gtagcgcaat
     3181 gccagacgca tgcgttgcgg ctcgcgcgtc agttgccagg ccagcagcgg actgcggcct
     3241 tgtagcggcc acagccagac gcacagccag ccggccagta tcccggtggg cacatcgata
     3301 aagtgatgct gccaggtggt cagcaccgat acgccgatca gggccatcca gccgtgcagc
     3361 aaaccgcgca gccacaggcc atgggtatgc cgggcgaaca tcacccagat cactaccagc
     3421 agggcgatat gcagggatgg cgcctggtta aagggtttgt cgaaacccat cagcacgtcg
     3481 aacatgccgc cgaacaggcc atccagttcg ggccgctcga aggtaaagcg cagcggccag
     3541 agcagaaagc aggcaatgca cagcacttgc acgctaagca ggcgcaggcc gtggcgatcc
     3601 atttcccgcc gggtggccgg cagtagaaag gacaacccat aaagcaggtc aattgaccag
     3661 taaggcgcga tagtccatgg ccatagcggc atatggcttt cccaggcaaa caccagggtg
     3721 ccgacgtcgt cccgttgcgc gcttagccag ttggcgaagc tgtagctggc gaagaacagc
     3781 ggcgccaaga gcagcaacca gagcacgccg cgcttccata acccggcttc gcggctaagg
     3841 ctaacttccg ccatcaggac acccgctgcg ccagggaaac gctgaagatg ccccactcgt
     3901 cgatacgctg ggtgatcttg cgaaagcctg cggcctcgac cagctgatcc atttcagcct
     3961 ggctacgtcg tcgcataacc catgcttcgc cgccgcggtg gctggtcagg gcgcgggcga
     4021 tcagctctag ctgcgggtgc cagggctgcc cggtgtacac cagataaccg ccctcctcga
     4081 cggctgccgc cagcccggaa agtgactcgc caaccatctg gttgctgccg aacagctcat
     4141 acaaaccgga gaccaccgcc agagtcggtt taggctctag tgccgcgagg tcggcacggt
     4201 cgaaagcatc gcctttgacg aaactagcga tatcgctcag gcccttctgt tcgatcagta
     4261 caccgccgtc gcgcacgttg atatcgctgt aatcgcgcaa caggatcgag tccggcttct
     4321 gctccatgcc ttccagagct tcgaggatgt agcggccatg gccggcggcg atatcgacga
     4381 tgcgcacgcc acggccctgc tcgcgcagct tgcccatggc caggcgcagc aactcttcgg
     4441 catgcagttt gcgctgacgg ataccgcgcc agccgatgga gttgaggtag ttctgatcga
     4501 tcagtctgcc gataggcgac ttgccgctgg gttggttgcg gtagacgtaa tccagggtgc
     4561 tgccggaatc gaagccagtg tcgaaaccca gtttcactcc gtcggccaga ctgctgccga
     4621 gccgcaggcc ggcgcgggtg gcgcgccagt aaaggtcgcc cagggagtta tgcggcagcg
     4681 gtgcggccag gctttccgat tcggcgcagg ttacgccgag gcggtcggca tcgagcaagg
     4741 aagggcgcgc taccggctct tcaaaacagc gcagcagaaa gcgctgggcg cgtttcaggg
     4801 cgtggctgcg gtcgcgttcg ccgagagtgt cgtggaagaa gccgggaagg atgtgcatct
     4861 ccttgcgcag gctgcccagg cgctcgaaga attgctgctg gggtttgcgg tgcaccacaa
     4921 agtcggcgcc cgaaatcagc agctgagtgg gaatctggat cgcctgagcg tctgcgacca
     4981 cgcgttcggc ggcgttatac aggcccagca gcatatttac cgagatcggt cgggcgatca
     5041 gcgggtcgtt aataaaggag gcaatccgct ccgggtcatg gctgaggtac ttggccttca
     5101 catagctgtt gacgaagaag ttgccgcgca gcgcgcgcat caacttgagg ccgggccggg
     5161 caaagggcac gtagagcttg accttgaacg ccggcgaggc gagcaccagg gcgcggatct
     5221 tcggtgcata gtcatgcgcc caggtggcga ccaccaccgc gccaacgctc tgcgccagta
     5281 ccgcaatatc ctccaccgcc aggccgtggt ggctgacgat atggtcgata aaggtctgca
     5341 catcgcgcac gctggtggcg aagcttgggc tgtcgccgcg ctcacctggc gacaggccat
     5401 ggccgcgcgc gtcccaggcg aaaaagtcga aatccgccat ttccagttca tcgaccagat
     5461 gggccatgcg cccgccgtgt tcgtggccgc ggtggaacat caccaccgcg cgctttggcg
     5521 cggcgccatc agtgttgctg gccggccagt ggcggtagaa cagctctacg ccgtcatggg
     5581 tggtaaagct gaattcttgg gcttcgcgca tcgagacatc cttatttcga gtcagttggc
     5641 tgcgccgctt cggccaggcc ctggcgtacg cggttgtaga gggtgtagag caacagggcg
     5701 agaatcaccg cgagcaggcc gttgagccag gcggcagaca gcaacccgca ggccacgccg
     5761 gcccccagca cgccgaagca gaaggcacgg tcgctcttgc ccatgggccc gtcgtaacgg
     5821 cgcgaggcac cgaccatagg cccgagcacg ccggcgtatt cgctgatcag ggccagcagc
     5881 acgaccagta ccaccagcag cggcgaaacc ccggggagca aggcgaaggg tagaaacagc
     5941 gcgctgtcgg cgatcaggtc gctgagttca ttgaggtagg cgccgagccg cgattgttgg
     6001 ccgaactcgc gggcgagcat gccgtcgacg gcattcagcg ccatgcgcag aatcatccac
     6061 agcgggatca gggcgaacag ccaggtgtgc tgaggcaagg ccagcagcag ggtgccgagc
     6121 agcacggaaa ccacggcggc gaacagggtg acttgattgg ccgtcacgcc tttgtcgtag
     6181 aggcgtttta cccccgggcg caggaggttc tgaaacgccg gcttgagctg gtaaatggag
     6241 agcatggcga agaattcctt ttcgctgctt gggcacgtcg atccagctta gccgctttgc
     6301 ctggtgcgca gactgataag actgtgcgca aacgcccgga tttgctggtt ggcagctacc
     6361 tgggcggtat gggggaaact tatcccccat cgcctgatat tccgtttcag gcgttaattc
     6421 tcgttaaatc aactgcttgc agcgcgtaat agcactttga cgctattcgg tgcacagctt
     6481 atccacaatt gctcaggccg gcgcttgggt ctccagcggc tggctttgcg catccagcga
     6541 gccgatatcg cgttgcgcct ggctgaccca ggcaaaggcg cggtcgttga gttcggcgat
     6601 ggcgcgcggg ccagtgcctt cggcgtacat cggcgggccg ataatcacct ggacggtgcc
     6661 aggggtcttg cgccagccct gcttgggcca gaactcacct gcgttgtggg caatcggcag
     6721 caccggcagg ttggcattca ctgcgaggga ggcgccacca cggctgaact tgccgatctg
     6781 gcccggtgga atacgcgtgc cttccggaaa taccagcacc caggcgccct gccccagacg
     6841 tttgtggcca agcttggcca gttgcttgag cgccgccttg gggttgctgc ggtcaatcgc
     6901 gatgggcttg agcatcgcca ttgcccagcc gaagaacggc acatacagca gctcgcgctt
     6961 gaccacctgg ctcagtggct cgaagtagcc gcagaggaag aaggtttccc aggtgctctg
     7021 gtgtttggcg aggatcacgc agggcttatc ggggatattc tccaggccct tgatctcgta
     7081 gttgatgccg accaccacct tggccagcca ggtggcacag cggcaccagt tctgcacgac
     7141 aaagcggtag cgcgcacgaa acggcagaaa gggcgcgacg aagacgctga gggtgcacca
     7201 gaagaaggag gtggaagcca gcagcaggta gaagaggaag gttctgaggg tctgcactgt
     7261 cgacatggga gcttttaccg tcgcaggcag tgcctgcttt ataagtggag aagctgatcg
     7321 gcaaccgccg ccagatcatc gaataccagg gtgcccgctg gcaatccttt cgccagggtg
     7381 cgttcgccct tgccggtctt taccaaaaca ggctgacaat cgacggcgcc ggccgctgtc
     7441 aggtcaccgc tgctgtctcc gacaaaccac acgcccttaa gtgctacgcc atagtacgca
     7501 gcaatctgct caagcatgcc gggttttggt ttgcggcagg cgcagccctc atccgggcca
     7561 tggatgcagt gcacgatcag gcccagctcg ccgccctgcg ccgccaccag ttcgcgcagg
     7621 cgcgcgtgca tggcttccag cgtggccagg tcgtaatagc cgcgggcgac cccggactga
     7681 ttggtggcca cggcgaccgt ccagccggct tgcgacaggc gcgcgatggc ctcaatggca
     7741 ccgggcagcg gaatccattc ggcgaggctt ttgatatagg cgtcggagtc gtaattgatg
     7801 acgccatcgc ggtcgagaat cagcagtttc atgcggtaat ccgtaaccgg ctggcggtga
     7861 tggccgccag ccggtaaagg tgatgatggg ctgcgcgcag cccatcggac tcgcggcagg
     7921 cgttagccga gtaccgagat atcagccacg ccgaggaaca ggccacgcag cttggccagc
     7981 agcgcatagc ggttggcccg cacggcaggg tcttcggcgt tgaccagtac ggcctcgaag
     8041 aaggcgtcca ccggctcgcg caggttggcc agctggctca gcgccaggtt gtactgacgg
     8101 gctgcggcga gcggctgcac cgcatgctcg gcctgctgaa tggcggcgtt gagggcgaac
     8161 tcgctggggt tgtcgaagtg atgcgcttcg acgctcgccg cgacctggcc ttcggccttg
     8221 ctcagtagat tcgacacgcg cttgttcgct gcagccagcg cggcagcctg cggcaatgca
     8281 cggaaggcct gcacggcctg tacgcgctga tcgaagtcca gcggcgaggt cgggctgacc
     8341 gcacgcacgg cctggtacac ggccacttcg acgccttcgt cctcgtaacg agcgcgcaga
     8401 cggtcgaaga taaagtcctg cacctgggga gccaggccgt cggccttgat cttgtcgccg
     8461 aactggccga tggcgaagct gacggcttca accaggtcca gatccagctg cttctcgatc
     8521 agaatacgca gcacgccgag ggccgcacga cgcagggcgt aggggtcctt gctgccggtg
     8581 ggcagcatgc cgatgccgaa gatcccgacc agggtatcca gcttgtcggc cagggccaag
     8641 gcagcaccgg tgagggtgct cggcagctcg gcgccggcgc cgcgcggcat gtactgctcg
     8701 ttcagcgcca gggcgacgtc ttcggcttca ccgtcgtgct tggcgtagta gtaaccggcg
     8761 atgccctgca tttccgggaa ttcaccaacc atctcactgg ccaggtcgca cttacagagg
     8821 atgccggcac gcgcggcgcg ggtggcgtcg ccaccgatgc gctgggcaat aaagccagct
     8881 agggcggaga cgcgctgggc cttgtcgtag accgagccga gctgagcctg gaacaccacg
     8941 ttggccaggc gctggttgaa gctttccagc ttctgcttct tgtcctgctt gaagaagaac
     9001 tcggcgtcgg tcaggcgcgg acgcaccacc ttctcgttgc cggaaatgat ctgcgccggg
     9061 tccttgctct cgacgttggc cacggtgata aagcgcggca gcagcttgcc gttgccatcc
     9121 agcaggcaga agtacttctg gttgtcctgc atggtggtga tcagcgcttc ctgtggcacc
     9181 tcgaggaagc gttcctcgaa ggagcagacc agcggcaccg gccactcgac cagggcgcta
     9241 acttcatcca gcagcgctgg cggcacgatg gcggtgccct gctgctcggc ggctagttgg
     9301 tcaacgcggg cagcgatttg cgcgcggcgc tcggcgaagt cggcaatcac ataggcgctg
     9361 cgcaggtctt cggcgtagtt ggccggcgcg ctgatgcgca cctcgtggtt ggcgtggaag
     9421 cggtggccac gggatacgcg accggcttgc tgggcgagga tttcgcag
//
LOCUS       DASRUM010000092         9935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_555946, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000092 DASRUM010000000
VERSION     DASRUM010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9935
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555946"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /gene="lysS"
                     /locus_tag="VFF22_06300"
     CDS             <1..950
                     /gene="lysS"
                     /locus_tag="VFF22_06300"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011532564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZX16461.1"
                     /translation="IAHIRSFLMKRDFLEVETPMLQTIPGGAAAKPFETHHNALDMQM
                     FLRIAPELYLKRLVVGGFEKVFEINRNFRNEGVSTRHNPEFTMLEFYQAYADYEDNMD
                     LTEELFRELAQLVLGSTDVPYGDKVFHFGEPFVRLSVFDSILKYNPELTAADLTDIDK
                     ARAIAKKAGAKVLGFEGLGKLQVMIFEELVEHKLEQPHFITQYPFEVSPLARRNDQNP
                     SVTDRFELFIGGREIANAYSELNDAEDQAARFMQQVADKDAGDDEAMHFDADFVRALE
                     YGMPPTAGEGIGIDRLVMLLTNSPSIRDVILFPHMRPQA"
     gene            1078..1791
                     /locus_tag="VFF22_06305"
     CDS             1078..1791
                     /locus_tag="VFF22_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZX16462.1"
                     /translation="MTAKPAQESATLVANAVAESVQYQGRKASRQGSEQRRQAILDAA
                     MRIIVREGVRAVRHRAVAAEAEVPLSATTYYFKDINDLITDTFAQFVERSAAHMGQFW
                     SSTEGLLREQVSRLDGSAEARRRLANEIARLAVEYVRQQLLTRREHLLAEQAFQQEAL
                     LNPRLHGLLRAHRQILLQGVTHFFEVLGSQQAEQDAKLLTAIIVRMEYQGLLDGVDHL
                     DSEEMLAMLKRYMHLVLGL"
     gene            1834..2385
                     /locus_tag="VFF22_06310"
     CDS             1834..2385
                     /locus_tag="VFF22_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16463.1"
                     /translation="MKAWRALLAVSFLLLGGCLVTFKDPIPANEAAPIPLLGEWSRKN
                     EWGEQLYLQITRAGSNVYKARSYVDSLDNLDSVEEYGFTVSHHGRRWYLSAGLPKSLG
                     ANFAIAGFELTTSNELVIYNLDVDRILHELERGTLQGEVIDGAEGDGVLISSPLEQVF
                     NYLDDQANSDVFIEVARYQRAGD"
     gene            2403..3698
                     /locus_tag="VFF22_06315"
     CDS             2403..3698
                     /locus_tag="VFF22_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavohemoglobin expression-modulating QEGLA
                     motif protein"
                     /protein_id="HZX16464.1"
                     /translation="MNSHAGLDDYQLIVRALSDRLVDAQTPIRVLDAVKWDDSIRSEF
                     LKHKGKKLPAVDRAYYANRPLPFDASAKKLEFQNIERDITRQLGQFNPVGKIMRRMCR
                     EYRMVIRMLEARGTEDFGLISQELYGAASDAFHAGDPTLADLGLMFSDFLNNIDKRGD
                     LKDEAKTLSAKEAVNLLQHRLNKVFGEEEGTIRVFESDGILADAAAGADYIKIRADAM
                     FNDRDVKALEVHEGLVHVATTLNGLNQPICTFLAKGPPSSTVTQEGLAILMEVITFAS
                     YPTRLRKLTNRTRAIHMAEEGADFLQVFDFYREQGFSQEDSYSNANRVFRGSTPNGLP
                     FTKDLSYLKGFIMIYNYIQLAVRKGKLEQIPLLFCGKTTLEDMRSLRQLVDEGLVVAP
                     KYLPPQFSDLNSLSAWMCFSNFLNHLSLDRIEADYANIL"
     gene            3854..4603
                     /locus_tag="VFF22_06320"
     CDS             3854..4603
                     /locus_tag="VFF22_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HZX16465.1"
                     /translation="MPSHQLDYEILGASAQSVEIILDPGETVIAEAGVMNYMTDGIRF
                     ETRMGDGSASGVLGKLWGMGKRMLTGESLFMTHFSNAGKTPARVAFAAPYPGTVVPIQ
                     LAEHGGTLICQKDAFLCAAYGTAVGISFNKRLGAGFFGGEGFILQKLEGDGLAFVHAG
                     GTVIRKELNNETLRLDTGCLVAFTSGIDYDIALAGGLKSMLFGGEGILLATLKGTGTV
                     WVQSLPFSRLAERIYEATFKAREEVRAGGNK"
     gene            4690..4992
                     /locus_tag="VFF22_06325"
     CDS             4690..4992
                     /locus_tag="VFF22_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16466.1"
                     /translation="MSEINPIPLILTGIGSICATVAVLGYYGYLHIAKPEDALLLSEF
                     TMLKTVPGEDYKVSLKPASQVAQCIDGVLVMFDLEQKGLSGVLVDRRKQAVRCNEP"
     gene            complement(5085..5873)
                     /locus_tag="VFF22_06330"
     CDS             complement(5085..5873)
                     /locus_tag="VFF22_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZX16467.1"
                     /translation="MRKHIMIPALLAISVGLAACSSQPNVNLEEAQSNFSSLQSDPQA
                     AKVAALETQDASEMLKRAEKAYLDKEDEAKVDQLAYLANQSVEVAKQTINLRSAEANL
                     QNSAAKRAEARLAARDAQIQQLQQSLNAKQTERGTLVTFGDVLFDLDRAELKPGGLGN
                     VNKLAQFLQENPERKVVVEGYTDSSGSASYNQQLSERRANSVRAALVKMGVGPERIVA
                     QGYGKEYPVADNSSSSGRAMNRRVEVTISNDNQQVAPRSSMGSN"
     gene            complement(5916..6332)
                     /locus_tag="VFF22_06335"
     CDS             complement(5916..6332)
                     /locus_tag="VFF22_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HZX16468.1"
                     /translation="METNSMKIQDNIRASNPLRNLKLAALLVGSSVVLAGCAGNPPSE
                     QFAVTQSAVNNAVSAGATEHAAVEMQSARDKLKQAELAMHDKDYEKAKRLAEQAEWDA
                     RVAERKAEAAKAERALQDANQGIEELRKEGMRGTQQ"
     gene            complement(6596..6925)
                     /locus_tag="VFF22_06340"
     CDS             complement(6596..6925)
                     /locus_tag="VFF22_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin assembly protein"
                     /protein_id="HZX16469.1"
                     /translation="MKIRELVQHWQENAQGRLSATPYQIRLDLEAAARLAALQEMYPK
                     HHVDELLGELLGAALEELEASLPYVKGSTVVASDEQGDPLYEDIGLTPRFLALSRQHL
                     QRLLAEK"
     gene            7081..9717
                     /gene="ppc"
                     /locus_tag="VFF22_06345"
     CDS             7081..9717
                     /gene="ppc"
                     /locus_tag="VFF22_06345"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HZX16470.1"
                     /translation="MAEIDARLREDVHQLGELLGNTISVQHGAAFLDKIERIRKGAKA
                     SRRGSQEGAEQLSATLNQLDDDELLPVARAFNQFLNLANIAEQYHRVRRRAPGEAQPF
                     EDNILAELLQRLCSNGHKGEALARQFGRLDIELVLTAHPTEVARRTLIQKYDAMAEQL
                     AAGDHSDLSAAEREQVKQRLQRLIAEAWHTEEIRRSRPSPVDEAKWGFAVIEHSLWHA
                     IPNVLRKADQVLHSATGLHLPLDAAPIRFASWMGGDRDGNPNVTASITREVLLLARWM
                     AADLYLRDVDNLAAELSMQQASPALLARVGESAEPYRSLLKQLRERLRATRSWAQAAL
                     NEQVPATAAVLQDNRELLEPLTLCYQSLHECGMGVIADGPLLDCLRRAATFGLFLVRL
                     DVRQDSSRHAAAMSEITDYLGLGRYAEWDEDARLAFLLKELENRRPLLPGHFKPSGET
                     EEVLATCREVASAPAASLGSYVISMAGAASDVLAVQLLLKEAGLQRPIRVVPLFETLA
                     DLDNAGPVISRLLGLHGYRARLHGPQEVMIGYSDSAKDAGTTAAAWAQYRAQETLVQV
                     CREQDVELLLFHGRGGTVGRGGGPAHAAILSQPPGSVMGRFRTTEQGEMIRFKFGLPD
                     IAEQNLNLYLAAVLEATLLPPPSPEPAWRELMDKLASDGVSAYRAVVREHPQFVDYFR
                     QATPEQELGRLPLGSRPAKRREGGVESLRAIPWIFAWTQTRLMLPAWLGWEAALDNAF
                     QRGEGELLGQMREHWPFFRTRIDMLEMVLAKADESIAQLYDERLVEPTLRPLGEHLRG
                     LLSQAVVAVLGLTGQSQLLAHSPETLESISVRNTYLDPLHLLQAELLARSRLRDSQAD
                     SPLEQALLVSVAGIAAGLRNTG"
BASE COUNT         1835 a         2948 c         3112 g         2040 t
ORIGIN      
        1 tcatcgcgca tatccgcagc tttttgatga agcgcgactt ccttgaagtg gaaacgccga
       61 tgctgcagac catcccaggc ggcgctgcgg ccaagccgtt cgagactcac cacaacgccc
      121 tggatatgca gatgttcctg cgtatcgccc cggagctgta cctcaagcgt ttggtggtgg
      181 gtggtttcga gaaggtgttc gagatcaatc gcaacttccg taacgaaggc gtctcgaccc
      241 ggcataaccc tgaattcacc atgttggagt tctaccaggc gtacgccgac tacgaagaca
      301 acatggacct gaccgaggag ctgttccgcg agttggccca gctggtgctc ggcagcactg
      361 acgtgccgta tggcgacaag gtgttccact tcggcgagcc gtttgtgcgt ctgtcggtgt
      421 tcgactcgat cctcaagtac aaccctgagc tgacagctgc cgacctgact gacatcgaca
      481 aggcccgtgc catcgccaag aaggccggtg ccaaggtgct gggtttcgaa ggcctgggca
      541 agctgcaggt gatgattttc gaggagctgg tcgagcacaa gctggaacag ccgcacttca
      601 tcacccagta ccccttcgaa gtctcgccgc tggcccggcg taacgaccag aacccgagcg
      661 tcaccgaccg cttcgagctg tttatcggcg gccgcgagat cgccaacgct tactccgagc
      721 tcaatgatgc cgaagaccag gccgcacgct ttatgcagca ggtggccgac aaggacgctg
      781 gcgacgatga agccatgcac tttgacgccg acttcgtccg cgccctggag tacggcatgc
      841 cacctaccgc tggtgagggt attggtatcg accgcctggt gatgctgctg accaactcac
      901 cgtcgattcg cgacgtgatc ctgttcccgc acatgcgccc gcaggcctga tgttgctaag
      961 ccgcctacgg gcggcttttt attgcctggc tttttattgt ctgcagatgg attatttttg
     1021 agatttactg actaagtcat tagtaatctt gaataaacga cagaggatcg tcttgctgtg
     1081 acagccaagc ccgcccaaga aagcgccacc ctggtggcca acgccgttgc tgagagcgtg
     1141 cagtatcagg gccgtaaagc cagccgtcag ggcagtgaac agcgccgcca ggccattctc
     1201 gatgccgcca tgcgcattat tgtgcgtgaa ggcgtgcgcg ctgttcggca ccgggcagtg
     1261 gccgccgagg ccgaagtgcc gctgtcggcg accacctatt acttcaaaga catcaacgac
     1321 ctgatcaccg acaccttcgc ccagttcgtc gagcgcagtg ccgcgcatat gggccagttc
     1381 tggagcagta ccgaaggtct gctgcgtgag caggtcagcc gcctggatgg cagcgccgag
     1441 gcgcgccggc gtctggccaa tgaaattgcc cggttggcgg tggagtacgt gcgccagcag
     1501 ttgctgaccc gtcgtgaaca cctgctggcc gagcaggcct ttcagcagga agctttgctc
     1561 aacccgcgtc tgcacgggct gctgcgcgcg caccggcaga ttctgctgca gggtgtgacg
     1621 cacttctttg aagtgctggg ctcgcagcag gcggagcagg atgccaagct cttgacggcg
     1681 attattgtgc gcatggagta tcagggcctg ctcgatgggg tcgaccatct ggacagtgaa
     1741 gagatgttgg ccatgcttaa acgttatatg catctggtac tgggtctgta gcaggcctag
     1801 gaacccagtt aatccgttca caaggagagc tgaatgaaag cctggcgcgc gctgcttgcc
     1861 gtgtccttcc tgttgcttgg tggctgtctg gtgaccttca aggacccgat tcccgccaat
     1921 gaagcagccc cgattccctt gcttggcgag tggtcgcgca agaacgagtg gggcgagcag
     1981 ctgtatctgc aaatcacccg tgccggttcc aatgtctaca aggcgcgctc ctatgtcgac
     2041 agcctggata acctcgacag cgttgaagaa tatggcttta ccgtgtctca ccatggtcgt
     2101 cgctggtacc tctcggcggg tttgccgaaa agcctgggtg cgaacttcgc catcgccggg
     2161 ttcgagctga ccaccagcaa tgagctggtg atctacaacc tggatgtcga ccgtattctc
     2221 cacgagctgg agcggggcac cttgcagggt gaggtgatcg atggcgcaga gggcgatggc
     2281 gtgctgatca gcagcccgtt ggagcaggtg ttcaactacc tcgacgatca ggccaattcg
     2341 gatgtattta ttgaagtcgc gcgttatcag cgggcgggcg attagtgggt gatggggaaa
     2401 acatgaacag tcatgccggg ttggacgatt atcagttgat cgtacgcgcg ctgtccgacc
     2461 gtctggtcga cgcgcaaacg ccgatacgcg tgctggatgc ggtgaagtgg gacgattcga
     2521 ttcgcagcga gtttctcaag cacaagggca agaagctgcc cgccgttgat cgtgcctact
     2581 acgccaaccg gccgctgcct tttgatgcgt cagcaaagaa gctggaattc cagaatatcg
     2641 agcgcgatat cactcgccag cttggccagt tcaacccggt gggcaaaatc atgcggcgca
     2701 tgtgccgcga gtaccgcatg gtcatccgca tgctcgaagc gcgtggcacc gaggacttcg
     2761 gcctgatcag ccaggaactc tacggcgcag cctccgatgc tttccacgct ggcgatccga
     2821 ctctggccga cctgggcctg atgttctccg acttcctcaa taacatcgac aagcgcggcg
     2881 acctcaagga cgaagccaaa accctgagcg ccaaagaggc ggtcaacctg ctgcagcatc
     2941 gtctgaacaa ggtgtttggc gaggaggagg gcaccattcg ggtgtttgag tccgacggta
     3001 ttctcgccga tgccgcagcc ggcgccgact acatcaagat ccgcgccgat gcgatgttca
     3061 atgaccgtga tgtaaaagcc ctggaagtgc acgagggcct ggtgcatgta gccactacct
     3121 tgaatgggct gaatcagccg atctgcacct ttctggccaa gggcccgccc tcgtccacgg
     3181 tgacccagga aggcctggcg atcctgatgg aggtcatcac cttcgcctcc tatccaaccc
     3241 gtttgcgcaa gttgaccaac cgcacccgcg ccatccatat ggccgaagag ggcgcggatt
     3301 ttctgcaggt cttcgacttt taccgtgagc agggctttag ccaggaagac agctacagca
     3361 acgccaatcg ggtattccga ggttcgaccc ccaacggcct gcctttcacc aaggacctgt
     3421 cctacctgaa aggcttcatc atgatttaca actacatcca gttggctgtg cgcaaaggca
     3481 agctggagca gattccgctg ctgttctgcg gtaagaccac tctggaagac atgcgctccc
     3541 tgcgccagtt ggtggatgaa gggctggttg tcgcacccaa gtacctgccg ccgcagttca
     3601 gcgacctcaa ctcgctatca gcctggatgt gcttctccaa cttcttgaac catctgagtt
     3661 tggatcggat cgaagccgat tacgccaata tcctctgatg gaataattcc cgctgtaagg
     3721 cgtaggagcg gcgggggcgc ctagcccatg cccgcgagcc cttttgcttg tatgacctat
     3781 cgcggtcatg gaccgctcct acaaaaccga gtcccatccg ggtagcttta cccgcatagc
     3841 caaggagccc tgcatgccca gccatcaact cgattacgaa atccttggcg cctccgcgca
     3901 gagcgtggag atcattctcg atccgggcga aacggtgatc gccgaggccg gggtgatgaa
     3961 ttacatgacc gacggcatcc gctttgaaac acgcatgggc gatggttcgg ccagcggtgt
     4021 gctgggcaag ttgtggggca tgggcaagcg catgcttacc ggcgagtcgt tgtttatgac
     4081 gcacttttcc aatgccggca aaaccccggc gcgagtggcc tttgccgcgc cgtacccagg
     4141 caccgtggtg cctatccagc tggctgagca tggtggcacg ctgatctgcc agaaggatgc
     4201 ctttctctgc gcggcctacg gcacggcggt cggcatcagc tttaacaagc gcctgggtgc
     4261 cggctttttc ggtggcgaag gttttatcct gcagaagctc gaaggtgacg gcctggcctt
     4321 tgtgcatgcc ggcggcacgg tgattcgcaa ggagctgaac aacgagaccc tgcgcctgga
     4381 caccggttgc ctggtggcct tcaccagcgg catcgactac gacatcgccc tggccggcgg
     4441 cctgaaaagc atgctgtttg gcggcgaggg cattcttctg gccacgctca agggcactgg
     4501 taccgtgtgg gtgcagagcc tgccgttctc gcgcctggcc gagcgcatct atgaagcgac
     4561 cttcaaagcc cgtgaggaag tgcgcgctgg cggcaataaa tgatgcggca atctgggcct
     4621 ttgcaggcgt gaatgtttgc tgcaagctgc gccgcgtgtt gcgccaattg aaaactgcga
     4681 gagcctgtta tgagtgaaat aaaccccatc ccgctgatcc tcaccggcat cggcagcatc
     4741 tgcgccacgg tggctgtgct cggctattac ggctatctgc atatcgccaa gccggaagat
     4801 gccttgctgc tgtccgagtt caccatgctc aagaccgtgc cgggtgagga ttacaaggtc
     4861 tcgctcaaac ccgccagcca ggtcgcgcag tgcatcgatg gcgtactggt gatgtttgac
     4921 ctggagcaga aaggcctcag cggcgtactc gtcgaccgcc gcaagcaagc cgtgcgctgc
     4981 aacgagccgt gatgctagcc gggtctcagg ttcaagtaca aaaaagccgc gccccgaaag
     5041 ggcgcggctt ttttaaccgg tgtgtcagcc ggtcacagcg gtactcagtt gctgcccata
     5101 gacgagcgcg gtgcgacctg ctggttgtcg tttgagatag tgacttccac acggcgattc
     5161 atggcgcggc ccgaagagct gctgttatcc gctaccggat actccttgcc gtagccttgg
     5221 gcgacgatgc gctccggacc tacacccatc ttcaccagtg cagcgcgaac ggagttggcg
     5281 cggcgttcgg acagctgctg gttgtagctg gctgagccgc tgctgtcggt atagccctcg
     5341 accacgacct tacgctccgg gttctcctga aggaactgag ccagcttgtt gacgttaccc
     5401 agcccgcccg gcttaagctc ggcgcggtca aggtcgaata gcacatcgcc aaaggtgact
     5461 aaggtgccgc gttcggtctg cttggcattc aggctttgct gcaattgctg gatctgcgca
     5521 tcacgcgctg ccaggcgcgc ttcagcacgc ttggcagcgc tgttttgcag gttggcttca
     5581 gcgctgcgca ggttgatggt ctgcttggcc acctccacac tctggttggc caagtaggcg
     5641 agttggtcga ccttcgcctc gtcttccttg tccaggtacg ctttctcggc gcgcttgagc
     5701 atttcgctcg catcttgggt ttctagggcg gcgactttgg ctgcttgcgg atcgctctgc
     5761 aggctggaga agttgctttg cgcctcttca aggttgacat tgggctgcga tgagcaggcc
     5821 gccaggccga cgctgatggc aagaagggca ggaatcatga tgtgtttacg catagcggtt
     5881 catccttaat aaagtgaggt catgaagggc gggcgttatt gctgcgtgcc gcgcatgcct
     5941 tccttacgca gttcttcaat gccttggttg gcgtcctgga gagcacgttc ggccttcgcc
     6001 gcctcagcct tgcgctctgc tacgcgggca tcccactcgg cctgttcagc caggcgcttg
     6061 gctttctcgt aatccttatc gtgcatggcc agctcagcct gcttgagctt gtcacgagcg
     6121 gactgcattt ccactgcagc atgctcggtg gcgcccgcgc tgacggcgtt gttgactgcc
     6181 gactgggtga cggcgaactg ttccgatggt ggattgccgg cacagccagc aagaaccacg
     6241 ctgctgccga ccaacaatgc ggccagtttc aggttacgca gagggttcga cgcgcggatg
     6301 ttgtcctgga tcttcatgct gttcgtctcc attggtgaac tccttgaaag tcctgaaaag
     6361 cccggccgtt atggctcggg aggcatcttt gcaaacgctg caaggcgatg ctgtaaatca
     6421 gcggcgaagt gccatgactc actatttccg accgaagggg tggtgcaatg gttcaggaga
     6481 atcgccagcc aggcccgctg tgcgcgggcg tgagccgctc tacagcggta ctttcaggct
     6541 aatagcaagg tgcttgcaga agacgccttg ctacacagaa agtgatggga ggcgctcact
     6601 tctcagcgag taggcgctgc aggtgttggc gtgagagagc gaggaagcgc ggcgtcaggc
     6661 cgatatcttc atacagtggg tcgccctgtt catcgctggc caccaccgtg ctgcctttga
     6721 cgtagggcag gctggcttcc agctcttcga gcgccgcgcc gagcaactcg cccagcaact
     6781 cgtcaacgtg gtgtttgggg tacatctctt gtagtgccgc caggcgcgct gcggcttcca
     6841 ggtcgagcct tatctgataa ggcgttgcgc tcagccgccc ctgggcattt tcctgccagt
     6901 gctgaacaag ctcgcgaatc ttcatgattg cctcgtgtct gttctgtgcc cgccgtgttt
     6961 tggcaaggct gcatagttga gactagtgcc tgggccgata agtgcggccg cagcgtcgcg
     7021 cacttgtaag aagttggcgc gctgggcact ctggaggcag gatcagcgga ggcaagactc
     7081 atggcagaaa tcgacgcgcg tctgcgcgag gacgtccacc agctcggcga gctactgggt
     7141 aacaccatca gcgtgcagca tggcgccgcc tttcttgaca agatcgagcg cattcgcaag
     7201 ggcgccaagg cctcgcgccg tggctcgcag gagggcgcgg agcaactcag tgcaaccctg
     7261 aatcaactgg acgatgacga gctgctgccg gtggcacggg cattcaacca gtttctcaac
     7321 ctggccaata tcgccgagca gtaccaccgc gtacgccgcc gcgcgccggg tgaagcgcag
     7381 ccgtttgaag acaatatcct cgccgagctg ctgcagcggt tgtgcagtaa cggccacaag
     7441 ggagaggctc tggcccgtca gttcggccgt ctggatatcg agctggtgct gaccgcacac
     7501 cccaccgaag tagcccggcg caccctgatc cagaaatacg acgcgatggc cgaacagctc
     7561 gcggccggcg accacagcga tctttcggcg gccgagcgtg agcaggtcaa gcagcgcctg
     7621 cagcggctga ttgccgaagc ctggcacacc gaggaaattc gccgcagccg accgagtccg
     7681 gtggatgaag ccaaatgggg cttcgcggtg atcgagcatt cgctctggca cgccatcccc
     7741 aatgtgctgc gcaaggccga tcaggtgctg cacagtgcca ccgggttgca cctgccgctg
     7801 gatgctgcgc cgattcgctt cgcctcgtgg atgggcggtg accgtgacgg caacccgaat
     7861 gtcaccgcaa gcattacccg cgaagtgctg ctgctggcgc gctggatggc ggctgacctg
     7921 tatttgcgcg atgtcgataa cctggccgcc gagctgtcca tgcagcaggc cagcccggcg
     7981 ctgttggcgc gggtcggcga gagtgccgag ccctatcgca gcctgctcaa gcagctgcgt
     8041 gagcgtttac gcgccacccg cagctgggct caggccgcgc tgaatgagca ggtacccgcg
     8101 actgcagccg tgctgcagga caatcgcgag ctgctggagc cgctgaccct gtgctaccag
     8161 tcgctgcatg agtgcggcat gggcgtgatc gccgatggtc cgttgcttga ttgcctgcgc
     8221 cgcgcggcga cctttggcct gtttctggtg cgtctggatg tgcgtcagga ctccagccgc
     8281 cacgccgccg ccatgagtga aatcaccgat tacctcggtc tgggtcgtta cgccgagtgg
     8341 gatgaagacg cgcgcctggc gtttctgctg aaagagctgg aaaaccgccg accgctgttg
     8401 ccggggcact tcaagcccag cggcgagacc gaagaagtac tggccacctg ccgtgaagta
     8461 gccagcgcgc cggcggcatc cctgggttcc tacgtgatct ccatggccgg tgcggcctcc
     8521 gatgtgctgg cggtgcaact gctgcttaaa gaagccgggt tgcaacggcc gatacgcgtg
     8581 gtgccactgt ttgagaccct ggccgatctg gacaacgccg gcccggtgat tagccggctg
     8641 ctggggctgc atggctaccg cgcgcgcctg catgggccgc aggaagtgat gatcggctat
     8701 tccgattcgg ccaaggacgc cggcaccacg gcagcggcct gggcgcagta ccgcgcccag
     8761 gaaactctgg tgcaggtctg ccgcgagcag gatgtcgaac tgctgctgtt ccacggccgt
     8821 ggcggcacgg tggggcgcgg tggcggcccg gcacacgcgg cgattctgtc gcagccgccg
     8881 gggtcggtaa tggggcgttt ccgcaccacc gaacagggcg aaatgattcg tttcaaattc
     8941 ggcctgccgg atatcgccga gcagaacctc aatctgtacc tggcggcggt gctggaagcc
     9001 accttgttgc cgccgcctag cccggaaccg gcctggcgcg agctgatgga caaactggcc
     9061 agcgatggcg tcagtgccta ccgcgcggtg gtgcgcgagc atccgcaatt cgtcgattac
     9121 ttccgccagg ccacgccgga acaggagctg ggccgcttgc ccctgggcag ccgtccggcc
     9181 aagcgtcgcg agggtggggt tgagagcctg cgcgccattc catggatttt cgcctggacc
     9241 cagacgcgcc tgatgctgcc ggcctggctg ggctgggaag cggcgctgga caacgccttt
     9301 cagcgcggtg aaggcgagct gctcgggcag atgcgcgagc actggccgtt cttccgcacc
     9361 cgtatcgaca tgctggaaat ggtgctggcc aaggccgacg agtccatcgc tcagttatat
     9421 gacgagcgtt tggtcgagcc gaccctccgt ccgctgggtg agcatctacg cggcctattg
     9481 tcgcaggcgg tggtcgcagt gctgggtttg accgggcagt cgcagctgct ggcgcacagc
     9541 ccggaaaccc tggagtcgat cagtgtgcgc aacacctacc tggacccgtt gcacctgctc
     9601 caggccgagc tgctggcgcg ctcccggctg cgcgatagtc aggctgacag ccctctggaa
     9661 caggcactgc tggtcagtgt ggcgggtatt gcggccggtt tgcgcaacac cggctagtgg
     9721 gctaatgagg gcggtggggc tttcgtcggt gcagatgtag tgcgtcggcg aaagcactac
     9781 ctgctagatg tggtcgcatg tgtatccgac tttcggtggc ttgtgcgcta tgggtgcgct
     9841 gtgtatcttg agcggccttt cggcactctg gcccagtttg gcagcgctgc ttgaacaatt
     9901 ctgagattgg ccccacgagg cgagtccgac gattt
//
LOCUS       DASRUM010000093        19244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1562106, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000093 DASRUM010000000
VERSION     DASRUM010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 19244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19244
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562106"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..2343
                     /locus_tag="VFF22_06350"
     CDS             124..2343
                     /locus_tag="VFF22_06350"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HZX16471.1"
                     /translation="MPDVILRLALPSPLRRLFDYRAPPGVPRSALQPGARLRVPFGRR
                     EMIGILIEVTEHSEVPADKLKPALQLLDAQSPLPPALFKLCLWTAQYYQHSLGDTLSW
                     ALPVLLRQGEPAEVRQQRFWSVTEGAQFDDPRLSRAPRQRDALKTLAQHPNGVAHPLL
                     SKLQLNKDSLDLLLEKGLVKVEVRRHAPSERHANWLAQPELPLNAEQRAASEAIRSGL
                     GSFNAFLLAGVTGSGKTEVYLQLIRETLEAGKQALVLIPEINLGPQTLARFEQRFNAR
                     IALLHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMQRPGLIIIDEEHDASYKQQ
                     EGLRYHARDLAVVRAHQENIPIVLGSATPSLESLHNAHSGRYAMLHLRERAGGAKQPR
                     FLRLDVKSRPLDSGISGPMQQAIGQTLQAGQQVLVFLNRRGFAPTLLCHDCGWLSECP
                     RCDARMTVHQRSRELRCHHCGHVERQPSNCPKCNNVDLRPVGAGTERAEERLEILFPD
                     VPVLRVDRDSTSRKEAMTTLFNTVQRGEPCILVGTQMLAKGHHFPRVTLVAILDADGG
                     LFSADFRASERMAQLIVQVAGRAGRAEEPGKVIIQSHLADHPLLVQLTEQGYFAFAEQ
                     ALSERRSAGLPPFCHLALLRAEAHKPGQAEGFLDDACSEAELLLNELKLSGIELLGPV
                     PAPMERRAGRYRAQLLVQANARAPLHRLLNTWMLALEQMPSGRAVRWSLDVDPIDLF"
     gene            2404..3783
                     /gene="dbpA"
                     /locus_tag="VFF22_06355"
     CDS             2404..3783
                     /gene="dbpA"
                     /locus_tag="VFF22_06355"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HZX16472.1"
                     /translation="MTNTAFSSLPLSPAMLANLESLGYTEMTPIQAQSLPVMLKGMDL
                     IAQAKTGSGKTAAFGIGLLNPLNPRFFGCQALVICPTRELADQVAKELRRLARSADNI
                     KILTLCGGVPFGPQIGSLEHGAQIIVGTPGRIQQHLSKGTLKLDGLNTLVLDEADRML
                     DMGFYDSIAEIISQTPSRRQTLLFSATYPTGIKQLSATFMRDPQQVKADALHDDTQIE
                     QRFYEIAPEERMEAVVKLLNCYRPQSCVAFCFTKQQCQEVVDHLTAKGISAAALNGDL
                     EQRDRDQVLAMFANRSLSVLVATDVAARGLDIDALDMVINVELARDSEIHIHRVGRTG
                     RAGEKGLAMSLVAPAEGHRAQAIETLQRAPLNWHPLSSLKHQGGAPLQPPMITLCIGA
                     GRKDKLRPGDILGALTGEAGIPGAQVGKIAIFDFQAYVAVERSVAMQALKCLSEGKIK
                     GRSLRIRTL"
     gene            3842..5077
                     /locus_tag="VFF22_06360"
     CDS             3842..5077
                     /locus_tag="VFF22_06360"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZX16473.1"
                     /translation="MRNILVIEDSPLVLKILEHLFRKETGFQPIFCASMGEAQVMLET
                     SAELFFAAIVDLNLPDAPDGEIVDVVMGYHLPCIVLSGTYNEQRRDQMLLKGVVDYVL
                     KESQHSYEYAFRLLHRLESNSEVKILIAEDSDATRHYIRHVLTPHRYQILEACDGDEA
                     LRILKEQPDIDLLVVDHSMPGLSGFDLVKLLRQKMKRHELIILGLSADVKGSLSAKFI
                     KHGADDFLRKPFCPEELNCRVMSTLERRDLLRALKQAAQFDALTGLNNRRAFYEHGQQ
                     LFQQARSGGRDLSVAMLDLDFFKRINDEHGHASGDTALIAFARAFSAAFPDALLGRLG
                     GEEFAMVSQQDAAALSETLDELRTHCTSLKYAIGAPPLSFSAGIYHGAPDDLESLLHQ
                     ADLRLYQAKNQGRGRNCWQ"
     gene            complement(5078..5815)
                     /locus_tag="VFF22_06365"
     CDS             complement(5078..5815)
                     /locus_tag="VFF22_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX16474.1"
                     /translation="MNGLRACLLSWVLLAPLSAHAAETLRLVADPWPPFNDQTLLNNG
                     VASDLVTTALTRAGYTTSYVQVPWERAVRGLQQDIYDVLINAWYTDERAVFGHYSQPY
                     LINRVRFLQRKGSQISFKQLPDLYPYSIAVVRGYAYSSEFDQDSRLTKVGVLGFEVGA
                     RMLQARRVQLTLEDELVARYHLSRSLAGIRDQLEFLPQPLSENGLHILISRQHPQHQQ
                     IADAFNKAIEEMRADGSYAQIFKRHGL"
     gene            5950..7134
                     /locus_tag="VFF22_06370"
     CDS             5950..7134
                     /locus_tag="VFF22_06370"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011063948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZX16475.1"
                     /translation="MLTPQVVIIGAGAAGLMCALTAAARGRQVLLLDHANKAGKKILM
                     SGGGRCNFTNMYCEPSNFLSENPHFCKSALARYTQWDFIGLVAKYGVPYHEKKLGQLF
                     CDNKASDILGLLLSECDEAGVELRLDTSVSDISKVDSGYQLQTSVGTVRCESLVIATG
                     GLSIPTLGASGFGYQVAKQFGHELLPTRAGLVPFTITDPQLKTLCTELSGTSVEDCVV
                     SCNGQSFKENILFTHRGLSGPAILQISSFWQPGEAISINLLPHIDLPEWLAEQQRERP
                     NSELKTLLAELFTKKMAGLLADNWFASKPMKQYTHAELKAIAEQLDDWQLVPAGTEGY
                     RTAEVTLGGVDTREVSSKTMESLKSPGLYFVGEVLDVTGHLGGFNFQWAWASGYAAAQ
                     YV"
     gene            complement(7163..7843)
                     /locus_tag="VFF22_06375"
     CDS             complement(7163..7843)
                     /locus_tag="VFF22_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HZX16476.1"
                     /translation="MRELYELCGSDPQLLFSPYCWRVRLALQHKGLDFESRPLIFTDK
                     APLACSGQTLVPVLRDGEQVVHDSVAIFAYLDRTYPANPLLGDGVAATRARLLEKVVF
                     QSVRMPLLKILLPRVQGVIAEADQAYFRSSREKALGMSLEAFSDPQAGEEALGKALQP
                     LEIWLAESAYLDGESPAGSDYLVAGLLFWAWCLGTQPWADGSAVDHWFRRLLTRYEAQ
                     YGPVPRAL"
     gene            8006..10177
                     /gene="yccS"
                     /locus_tag="VFF22_06380"
     CDS             8006..10177
                     /gene="yccS"
                     /locus_tag="VFF22_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HZX16477.1"
                     /translation="MRAPAFRHSLRRLWAQDKFSYSLRVFIALSGALLLCWSQGWMHA
                     LIPLFLGVIASALAETDDSWQGRVMALLVTLGCFSMAAYSVEFLFPYPWLFVCALALS
                     SFALTMLGALGERYATLGAATLILAVYSMIGVEQRGGAAGLWQEPLLLVAGAAWYGLL
                     SVLWNALFANQPVQHSLARLFRELGQYLKLKAALFEPLRQLDVEERRLALAKQNGRVV
                     AALNAAKEIILHRVGNGRPGPKVSRYLKLYFLAQDIHERASSSHYPYNELAEAFFHSD
                     VLFRCQRLLRLHGEGCQRLAQAIELRQPFEYRELCRQALDDLHDSLEHLRVQSNPAWR
                     HLLRSLGALAGNLRSLDALLGNASNPDAMAEQQDSSLLDREPQSLKDVWERLRLQLTP
                     TSLLFRHALRLSIALAAGYGVLHWIHPTQGYWILLTTLFVCQPNYGATRLKLVQRISG
                     TVLGLLLGWALFDLFPNPLVQALFAVVAGVVFFALRSSRYTLATAAITLLVLFCFNQV
                     GDGYGLFIPRLVDTLLGSLIAGLAVLLILPDWQGRRLNRLLASTLSCNSRYLQQIIAQ
                     YASGKRDDLAYRLARRNAHNADATLSTTLGNMLMEPGHFRKEADIGFRFLVLSHTLLS
                     YLSGLGAHRGETSLSGNDNPLLTSASQLAASLEEIASCLNEERPVAIHSDAEEQLAAE
                     LEQLSDDLDDQQRLVQTQLALICRQLGPLRTLAAHLLKSHA"
     gene            10174..10656
                     /locus_tag="VFF22_06385"
     CDS             10174..10656
                     /locus_tag="VFF22_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HZX16478.1"
                     /translation="MNKSQLLQQVIARLEDDLLQAQQAALTAYETATHEENIAENKYD
                     TLGLEASYLATGQARRAAAIRQTLANWKQLVLRGFDPQLGIQLGALIELSDDQDRRQR
                     LFLGPDGAAMKLQQDGQAVQVIGPQSPLGQVLLGKTIGDEVTLRVASGVQLLEILSVD
                     "
     gene            complement(10855..12510)
                     /locus_tag="VFF22_06390"
     CDS             complement(10855..12510)
                     /locus_tag="VFF22_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX16479.1"
                     /translation="MPSHAPRLSTRTTLYAGYASLLALMLVIAIVSLYNLADSNKGFS
                     DYVNGVGNRARLANELSNAAKDRAIALRNLALNGNAATRGQQRTAIEAHEKTVANSLA
                     ALRQAVTTQDGVSAAGRELFSRIEQVESRYSPVAQAIAEHLFNGEDAQALRMVSEQCT
                     PLLEELTQVISEYLNNTNKMADQLVVANDLHYHQQRNLLIIITALAFVLAAVLGHWIS
                     RNLLRALGAEPSELNQLAQRVAEGDLRVGERNSEPPKDSVLAALQRMQQNLREMTQGI
                     EHSSQIVAQSSQELSQSSLRNAESVNQAQLEVEQIVTAIHQMAATVQDVARNAEAAAG
                     AAQEADRAALHSQQKASQAVSLIDELAETIEQSNVAMERLKNESSNIGSVLDVIKSVA
                     DQTNLLALNAAIEAARAGDAGRGFAVVADEVRSLARRTQEATTEIEGLIAGLQRIADE
                     TSQHMHRCKHSSEQSVSGVSEAGEAVTRIVSMIERITGMNQQIAAASEEQSTVAEQIS
                     RGIVTVRDSSEQSAAAFRSAQQESESLSRTSVALRDNISRFQL"
     gene            complement(13011..13721)
                     /locus_tag="VFF22_06395"
     CDS             complement(13011..13721)
                     /locus_tag="VFF22_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX16480.1"
                     /translation="MLLIAPLAARELLAVGSNFPGVFEQNGSGYSGLATSVLRQALEP
                     LGYQVRFELHPWARAQHMVARGEADILIGPYKNAAREQLFAFSALPFYRDHIVFYRQR
                     GSGLRWHGDYQQLFGRRIGVVRGWFYGAHFDSQRAQLELVTVERVENGLKMLGAGRLD
                     LLASNQRNTQSVLLALGLVDKHEQLEPPIDLQDGYFAFPRDERFGPLREAFDRALQQM
                     VEQGRLEQLAADWQVDIP"
     gene            13922..14155
                     /locus_tag="VFF22_06400"
     CDS             13922..14155
                     /locus_tag="VFF22_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2789 domain-containing protein"
                     /protein_id="HZX16481.1"
                     /translation="MQSNTPELETLFQQLGLEHDPISIDLFIKRHSPLAEDCLLADAP
                     FWDKAQADFLRQQLLADAEWAEVVDQLNLLLRG"
     gene            complement(14138..14662)
                     /locus_tag="VFF22_06405"
     CDS             complement(14138..14662)
                     /locus_tag="VFF22_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZX16482.1"
                     /translation="MTPLKYLQGYPAQLQDKIRQLIAEQRLGDYLARSYSGKHAIQSD
                     KALYAYIQGLKQEHLRNAPAIDKVLYDNRLDLTHRALGLHTAISRVQGGKLKAKKEIR
                     VASLFREAAPEFLQMIVVHELAHLKEAEHNKAFYKLCEYMLPGYHQLEFDLRVYLTWR
                     ELPGSLGELSAPQQ"
     gene            complement(14743..15843)
                     /locus_tag="VFF22_06410"
     CDS             complement(14743..15843)
                     /locus_tag="VFF22_06410"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZX16483.1"
                     /translation="MLNTSRALLLLGLLGSSLAHAMDYRIETVTEGLEHPWSLAFLPD
                     GRMLVTERAGRLRIIEQDGSLTPQPVGGVPEAFVAAQAGLMEVLLDPEFASNQQLYLS
                     YAYGTAQANNTRLAKARLVDGQLQDVQVLFSALPAKSGAAHYGGRMAWLPDNTLVLTL
                     GDGFDLREQAQDPTNHIGKIVRLNRDGSIPKDNPLVGQAGAAAEIYSLGHRNVQGIVY
                     DNQLQRLYSHEHGPRGGDELNLIEPGNNYGWPLITFGVDYTGAQISPYTELPGLQQPL
                     LQWTPSVAPSSLTQYRGALFPEWQGDLFAATLAERSVRRIRLQEGKLAGEEVLFEELE
                     ERIRDVRGGPDGALYLLTDSADGRLLRVVPSQ"
     gene            complement(15873..16328)
                     /locus_tag="VFF22_06415"
     CDS             complement(15873..16328)
                     /locus_tag="VFF22_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16484.1"
                     /translation="MPSKLRTLLYFFLAVLLASVMGSVLQTQFNLANLQALGAPMPLG
                     VRVQTTCLDLLGFSPTFAVLVILGFIFALPAASRLARAWPTWRWLLFTLAGALAVLAA
                     MALANALLPMPTLIGANRSLAGTLGLMVCGSLGALLFALLARRVPHAQS"
     gene            16506..17471
                     /locus_tag="VFF22_06420"
     CDS             16506..17471
                     /locus_tag="VFF22_06420"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZX16485.1"
                     /translation="MKKALPFSALIIALLAIAFSLGPVPPVDTQLTPTQLPADLERYL
                     AETEARYPDITPGAEKTIVWAHSDQRQTDLAIIYLHGYSATRQETAPLSDQLAQQLGA
                     NLFSTRLSGHGRSGTAMAEASVHDWLQDSQEALQIGQRLGKQVLVIGTSTGATLATWL
                     ALQSDNPQVLAYIMVSPNFAPKDPAATVLTWPWAKHFVPLLLGPEHHWQPRNAEQARY
                     WSHRYPVQALLPMMALVKYVREQPLEQINAPILTLYSKDDQVVSAPAIEAAFARFSSA
                     HKQLIALKDTQDPSHHVLAGDILSPNDTPRVQAAILAFLANLPPR"
     gene            17999..18682
                     /locus_tag="VFF22_06425"
     CDS             17999..18682
                     /locus_tag="VFF22_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase family 7 protein"
                     /protein_id="HZX16486.1"
                     /translation="MIDLATWNLTIPVGVPAATISTELLVGGYQDHYFQSKEASIFFW
                     APVNGTATSNASYPRSELRETFADGKLRNWTYPEADHFLRAALKVSQVPSTGKLVIGQ
                     IHAHKSTMPLLKLEYQYKAYSASGNIVAKVRIAPDAPIQVVTLANGIPLNQRFTYVLH
                     LTPKGTLAININGVKWSTRLDSVWASKPLYFKAGVYTQDNTGYETEAGAARFDKLSIE
                     HRPLPTTAP"
     gene            complement(18794..>19244)
                     /locus_tag="VFF22_06430"
     CDS             complement(18794..>19244)
                     /locus_tag="VFF22_06430"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HZX16487.1"
                     /translation="PPTGDILAIDRIKEIHKRIPNTHLVMHGSSSVPQEWLAIINQYG
                     GDIKETYGVPVEEIVEGIKYGVRKVNIDTDLRLASTGAMRRLMATNPSEFDPRKFFGA
                     TVTAMRDICIARYEAFGTAGNASKIKPISLEGMFQRYAKGELAAKVN"
BASE COUNT         3804 a         6234 c         5699 g         3507 t
ORIGIN      
        1 acactattgt caggtaccgc acggaattag gccggcgata ataccagagc accgcagcga
       61 cgcaaggccg gccgtcgact ggctgcatgc taagatcgcg ccctgcaacc tggagccctt
      121 cgcgtgcccg acgttattct gcgcctcgcc ctgccctcgc cgctgcgccg cctgttcgac
      181 taccgtgccc cgcccggtgt gccgcgcagc gccttgcagc caggcgcgcg tctgcgtgtg
      241 ccctttggtc ggcgcgagat gatcggcatc ctgatcgagg tgaccgagca cagcgaagtc
      301 ccggccgaca aactcaagcc cgccctgcaa ctgctcgacg cccaatcgcc gctaccgccc
      361 gcactgttca agctgtgcct gtggaccgcg cagtattacc agcacagcct cggcgacacc
      421 ctgagctggg cgctgccggt gctgctgcgc cagggcgaac cggcggaagt gcgccaacag
      481 cgcttctggt cggtcactga aggcgcacag ttcgatgatc cacgcctgag ccgcgcgccg
      541 cgccagcgtg atgcgctgaa aaccctggcc caacacccca acggcgtcgc ccatccgctg
      601 ctgagcaagc tgcagctgaa caaggacagc ctcgacctgc tgctggaaaa aggtctggtc
      661 aaggtcgaag tgcgccgcca tgcgccgagc gagcgccacg ccaactggct ggcccaaccg
      721 gaactgccgc tgaatgccga acagcgcgcg gccagcgaag cgatccgctc ggggctcggc
      781 agcttcaatg cctttctcct cgcaggggtg accggcagcg gcaagaccga ggtgtatctg
      841 cagctgatcc gcgaaactct ggaggcaggt aaacaggccc tggtgctgat cccggagatc
      901 aacctcggcc cgcagaccct ggcgcgcttc gagcagcgct tcaacgcccg tatcgccctg
      961 ctgcattcgg cagtgaatga ccgcgaacgc ctggacgcct ggttggcggc gcgcgatggc
     1021 gaggccgata tcattatcgg cacccgctca gcactgttca cccccatgca gcggccgggg
     1081 ctgatcatca tcgacgaaga acacgacgcc tcctataaac agcaggaagg cctgcgctac
     1141 cacgcccgcg acctggccgt ggtgcgcgcc catcaggaaa acatcccgat tgtcctgggc
     1201 tcggccacgc cctcactgga aagcctgcac aacgcccata gcggccgtta cgccatgctg
     1261 cacctgcgcg aacgcgccgg cggtgccaag caaccgcgct ttctgcgcct ggatgtaaaa
     1321 agccggccgc tagactccgg catctccggc cccatgcagc aggccatcgg ccagaccctg
     1381 caggccggcc agcaggtgct ggtgttcctc aaccgccgcg gtttcgcccc gaccctgctc
     1441 tgtcacgact gcggctggct gtccgagtgc ccgcgttgtg acgcgcggat gaccgtgcac
     1501 cagcgttcgc gcgagctgcg ctgccatcac tgcggacatg tcgagcgcca gccgagcaac
     1561 tgcccgaagt gcaacaacgt cgacctgcgc ccggtcggcg ccggcaccga gcgcgccgaa
     1621 gaacgcctgg agattctctt ccccgacgtg ccggtattgc gcgtggaccg cgacagcacc
     1681 tcgcgcaagg aggccatgac caccctgttc aacaccgtgc agcgcggcga accatgcatc
     1741 ctggtcggca cgcagatgct cgccaagggt caccacttcc cccgcgtgac cctggtggcg
     1801 attctggatg ccgatggcgg cctgttttcc gccgatttcc gcgccagcga gcgcatggct
     1861 cagctgattg tgcaggtcgc tgggcgtgct gggcgcgccg aggagccggg caaggtgatt
     1921 atccagagcc acctggccga ccatccttta ttggtgcagc tgaccgagca gggctacttc
     1981 gccttcgccg aacaggccct gagtgaacgg cgcagcgctg gcctgccgcc gttctgccac
     2041 cttgccctgc tgcgcgccga agcgcacaaa cccggccagg ccgaaggctt tctcgacgac
     2101 gcctgcagcg aagccgagct gttattgaac gagctgaaac tcagcggcat cgaactgctc
     2161 ggcccggtgc ccgcgccgat ggaacgccgc gccggtcgct accgcgccca gctgctggtg
     2221 caagccaacg cccgcgcgcc actgcaccgc ttgctcaata cttggatgct ggccctggaa
     2281 cagatgccca gcggccgcgc ggtgcgctgg tcgttagatg tcgaccccat cgatctgttc
     2341 taagacaggc cgccgcgccc agcctgttac actgcacgcc atttcgttta gcaggcctgt
     2401 accgtgacca acaccgcttt ctcttctctg ccgctttccc ccgccatgct ggcgaacctc
     2461 gagtcgctcg gttacaccga gatgaccccg attcaggcgc agagcctgcc ggtaatgctc
     2521 aagggcatgg acctgatcgc ccaagccaag accggcagcg gcaagacagc cgccttcggt
     2581 atcggcctgc tcaacccgct caatccgcgc ttcttcggct gccaggcact ggtgatctgc
     2641 ccgactcgtg aactggccga ccaagtcgcg aaagagctgc gtcgcctggc acgctcggcc
     2701 gacaacatca agattcttac cctatgcggc ggcgtgccct tcggcccgca gatcggctcg
     2761 ttggagcacg gtgcgcagat catcgtcggc accccagggc gcatccagca gcacctgagc
     2821 aaaggcaccc tgaagctaga cggcctgaat accctggtac ttgatgaagc cgatcgcatg
     2881 ctcgacatgg gcttctacga cagcatcgcc gaaatcatca gccagacccc gagccgtcgg
     2941 cagaccctgc tgttttctgc cacctatccg actggcatca agcagctgtc cgcgaccttt
     3001 atgcgcgacc cgcagcaggt caaagccgac gccctgcacg acgatacgca gatcgagcag
     3061 cgcttctacg aaatcgcccc ggaagagcgc atggaggcgg tggtcaagct gctcaactgc
     3121 taccgtccac agtcctgtgt ggccttctgc ttcaccaagc agcagtgcca ggaagtggtt
     3181 gaccacctca ccgccaaagg catctcggcc gccgcactga atggtgatct ggagcaacgc
     3241 gaccgtgatc aagtgctggc aatgtttgcc aaccgcagct tgtcggtatt ggtggccacc
     3301 gatgtggccg cacgcgggct ggatatcgat gcgctggaca tggtgatcaa cgtcgaactg
     3361 gcgcgcgact cggaaattca tattcaccgc gttggccgta ccggtcgcgc tggcgaaaag
     3421 ggcctggcca tgagcctggt tgcccccgcc gaaggtcatc gcgcccaggc catcgaaacc
     3481 ctgcaacggg cgccgctcaa ctggcatccg ctgagcagcc tgaaacacca gggtggtgcg
     3541 ccgctgcagc cgccgatgat taccctgtgc atcggcgcag gtcgtaaaga caaactgcgc
     3601 cctggcgata ttcttggcgc gctgactggc gaggcgggga tccctggcgc gcaggtcggc
     3661 aaaatcgcca tcttcgattt ccaggcttac gtggccgttg agcgcagtgt ggccatgcag
     3721 gcactcaagt gcctcagtga aggcaagatc aagggccgct cgctgcgcat ccgtacccta
     3781 tagttgcagt agacaaacag acggctgcgg tgacgtggcc agcgacagag gagtagcacc
     3841 cttgcgcaat attctggtga tcgaagacag ccccctggtg ctgaaaattc tcgaacatct
     3901 gtttcgcaag gagaccggct ttcagccgat cttctgcgcc tccatgggcg aagcccaggt
     3961 gatgctggaa acctcggccg agctgttctt cgccgccatc gtcgacctca acctgccgga
     4021 cgccccggac ggcgagatcg tcgatgtggt gatgggctat cacctgccct gtatcgtgct
     4081 cagcggcacc tacaacgagc agcgccgcga ccagatgttg ctcaagggcg tggtcgacta
     4141 cgtgctcaag gaaagccagc actcctatga gtacgccttc cgcctgctgc accgcctgga
     4201 aagcaacagc gaagtgaaga tcctgatcgc cgaggattcc gacgccaccc gccattacat
     4261 ccgccacgta ctgaccccgc accgctacca gattctcgaa gcctgcgacg gcgacgaagc
     4321 cctgcgcatc ctcaaggaac agccggatat cgacctgctg gtggtcgacc acagcatgcc
     4381 ggggctcagc ggtttcgatc tggtcaaact gctgcggcaa aagatgaagc gccacgagct
     4441 gatcattctc ggcctatcgg ctgatgtgaa gggctcgctc agcgccaaat ttatcaaaca
     4501 cggcgccgac gatttcctgc gcaaaccctt ctgcccggaa gagctcaact gccgggtgat
     4561 gtcgaccctg gaacgccgtg atctactgcg cgccctcaaa caagccgcgc aattcgacgc
     4621 ccttaccggc ctgaacaacc gccgcgcgtt ctacgagcac ggccagcaac tgttccagca
     4681 ggcccgctcg ggcggccgcg accttagcgt ggcgatgctc gacctggact tcttcaaacg
     4741 gatcaacgac gaacacggcc acgccagcgg cgatacggcg ctgattgcct tcgcccgcgc
     4801 gttttccgcc gccttccccg acgccctgct cggccgcctg ggtggtgaag agttcgccat
     4861 ggtcagccag caggatgccg cagccctcag cgaaaccctc gacgaactac gcacgcactg
     4921 caccagcctg aaatacgcta taggcgcacc gcctctgtca ttcagcgccg gcatctacca
     4981 tggcgccccg gacgacctgg aaagcctgct gcaccaagcc gacctgcgcc tgtatcaggc
     5041 caagaaccag gggcgcgggc gcaactgctg gcagtagcta caaaccgtga cgcttaaaga
     5101 tctgcgcgta gctgccatcg gcgcgcattt cctcaatcgc cttgttgaag gcatcggcaa
     5161 tctgctgatg ctgcgggtgt tggcggctga tcagaatatg caggccgttc tcgcttaacg
     5221 gctgcggcag gaactccagc tgatcacgaa tcccggccag gctgcggctc aggtgatagc
     5281 gcgccaccag ctcgtcctcc agggtcaatt gcacccgtcg cgcctgcagc atgcgcgcac
     5341 ccacttcaaa gccgagcacg ccgaccttgg tcaaccggct gtcctgatca aactcactgg
     5401 aataggcata accgcgcacc acagcaatgc tgtaggggta caggtcgggc aattgcttga
     5461 aactgatctg gctgcccttg cgctgcagaa aacgcacgcg gttgatcaga tagggctgcg
     5521 agtaatggcc aaaaaccgcc cgctcatcgg tgtaccaggc gttgatcagc acatcataga
     5581 tgtcctgctg caagccccgc actgcgcgct cccacggcac ctgaacatag ctggtggtgt
     5641 aaccggcgcg ggtcagagcg gtggtcacca gatcggaggc cacgccgttg ttcagcagcg
     5701 tctggtcgtt gaatggcggc caggggtccg ccaccaaccg caaggtctct gcggcatggg
     5761 cagacagagg cgccaacaac acccacgaca gcaggcaggc acgcagtcca ttcatcaacg
     5821 gcggttccag agaaagcgga aagctaatcc tagtcgatca ctggcgcagc cacaccccac
     5881 tgaccatagg ctaagatgcg cgctgttttg cgcccgccgt ggcgcgtgtc gcaggttgag
     5941 gatattgctg tgcttacacc ccaggtcgtg attatcggag ccggtgccgc cgggcttatg
     6001 tgcgcgctaa cggctgccgc gcgcggccgt caggtgttgt tgctggatca cgccaacaag
     6061 gccggcaaga agatcctgat gtccggcggt ggacgctgca atttcaccaa tatgtactgc
     6121 gaaccgagca atttcctctc ggaaaacccg cacttctgca aatccgctct ggcgcgctac
     6181 acccagtggg actttatcgg cctggtggcc aaatacggcg tgccatacca cgagaagaaa
     6241 ctcggccagc tgttctgcga caacaaggcc agcgacatcc tcggcctgct gctgagcgag
     6301 tgcgatgaag cgggtgttga gctgcgtctg gacacgtcag tcagcgacat cagcaaagtg
     6361 gacagtggct accagttgca gaccagcgtt ggcaccgtgc gctgcgaatc gctggtgatt
     6421 gccaccggcg gcctgtcgat tccgaccctg ggggccagcg gttttggcta tcaggtggcc
     6481 aaacagttcg gccatgagct gctgccaacc cgcgccggcc tggtgccttt caccatcacc
     6541 gacccgcaac tcaagacgct gtgcaccgag ctgtccggca cctcggtgga agactgcgtg
     6601 gtgagctgca acgggcagag cttcaaggaa aatatcctgt ttacccaccg cggcttaagc
     6661 ggcccggcga ttctgcagat ttcctcattc tggcagccgg gcgaggccat cagtatcaac
     6721 ctgctgccgc atatcgacct gcctgagtgg ctggccgagc agcagcgcga gcggcccaac
     6781 agcgagctga aaaccctgct cgccgagctg tttaccaaga agatggccgg gctgttggcc
     6841 gacaactggt tcgcctccaa gccgatgaag cagtacaccc acgccgagct caaggcaatt
     6901 gccgagcagc tcgatgactg gcaactggtg ccggccggca ccgagggcta ccgcaccgcc
     6961 gaggttaccc tgggcggcgt ggacacgcgc gaggtgtcgt caaagaccat ggagtcgctg
     7021 aaatcgccgg ggctgtattt tgttggcgaa gtgctggatg tcaccggcca cctgggcggt
     7081 ttcaacttcc agtgggcctg ggcctctggc tatgcggcgg cacaatacgt ctgaagcatc
     7141 agcaccaggc ttagcagcgc ggttacagcg cgcgcggaac agggccgtat tgcgcctcat
     7201 agcgcgtcaa taaacggcga aaccagtgat ccaccgccga gccatcggcc cacggctgcg
     7261 tacccaggca ccacgcccag aacaacagcc ccgccaccag ataatcgcta ccggctggcg
     7321 actcaccgtc tagataagcg gactcagcca gccagatctc aagcggctgt agggctttac
     7381 ccaaggcttc ctcacctgcc tgcggatcgg agaatgcctc caggctcatg cccagagcct
     7441 tttcccggct gctgcgaaaa taggcctggt ccgcctccgc gatcactccc tgcacacgcg
     7501 gcagaagaat ttttagcagc ggcatgcgca cactctgaaa caccactttt tccagcagcc
     7561 gcgctcgcgt agccgccacg ccatcaccaa gtagcgggtt cgccggatag gtgcggtcca
     7621 ggtaggcaaa gatcgccacg ctgtcatgca ccacctgctc accgtcgcgc agcaccggca
     7681 ccaaggtctg cccggagcac gccagcggtg ccttatcggt aaagatcagc ggccggcttt
     7741 cgaaatccag tcctttgtgc tgcaaggcca ggcgcacccg ccagcagtat ggggagaaca
     7801 gcagctgagg gtcactgccg cagagttcat agagctcacg catgggaggc ctagtagtag
     7861 attgtgaatg gcggatagtg ccggggcgca gcctgatttg ccaagtcgcg ttcaggccca
     7921 ggcttctcag gcgcagccag gctcagtaac ctgcatcggt aaagcggcgt ctatcggtta
     7981 cccccctcac aaggctaatt cctgcatgcg cgcacccgcc ttccgtcact ccttgcgccg
     8041 cctctgggct caggacaaat tcagctacag cctgcgggtg ttcattgccc tgagtggggc
     8101 gttgttgctg tgctggtcgc agggttggat gcatgcgctg attccattgt ttctcggggt
     8161 gatcgccagc gctctggcgg aaaccgacga cagctggcaa ggccgtgtga tggcgctgct
     8221 ggtcaccctc ggctgcttca gcatggcggc ctattcggtg gaatttctgt tcccctaccc
     8281 ctggttgttc gtctgtgccc tggcgctgtc gagctttgcc ctgaccatgc tcggcgccct
     8341 cggcgaacgc tacgccaccc tcggcgcagc cacgctgatt ctcgcggtat acagcatgat
     8401 cggcgtggag cagcgcggcg gcgcggcggg cctctggcag gagccgctgc tgttggtggc
     8461 gggcgcagcc tggtatggcc tgctgtcggt gctgtggaat gcactgttcg ccaaccagcc
     8521 agtgcagcac agcctggcgc ggctgtttcg tgagctgggc cagtacctca agctcaaggc
     8581 ggcgctgttc gagccgctgc gtcagctgga cgtggaagag cgccgcctgg ccctggccaa
     8641 acagaacggt cgggtggtgg ccgcgctgaa cgcggccaag gaaatcatcc tgcaccgggt
     8701 cggcaatggc cggccggggc cgaaggtcag ccgctacctg aagctatact ttctcgccca
     8761 ggacattcac gaacgcgcca gctcttcgca ctacccttac aacgagttgg ccgaggcttt
     8821 cttccacagc gatgtgctgt tccgctgcca gcgcctgctg cgcctgcatg gcgagggttg
     8881 ccagcgcctg gcccaggcca ttgagctgcg tcagccattc gaataccgcg agctgtgccg
     8941 ccaggcgctg gacgacctac acgactccct tgaacacctg cgcgtgcaaa gcaacccggc
     9001 ctggcggcat ctgctgcgct ccctcggcgc gctggccggt aacctgcgca gcctggatgc
     9061 gctgctgggc aacgccagca accccgacgc catggcggag cagcaggaca gcagcctgct
     9121 cgatcgcgag ccacagtcac tcaaggatgt ctgggaacgc ctgcgtctgc agctgacgcc
     9181 aacctcgctg ctgtttcgcc acgccctgcg cctgtccatc gccctagccg ctggttacgg
     9241 cgtgctgcac tggattcatc caactcaggg ttactggatt ctgctgacaa cgctgttcgt
     9301 ctgccagccg aactacggcg cgacccggct caagctggtg caacgcatca gcggcacggt
     9361 gctcggcctg ctgcttggct gggcgctgtt cgacctgttc cccaatccgc tggtgcaggc
     9421 gctgttcgcg gtggtcgctg gggtggtgtt ctttgccctg cgcagcagcc gctacaccct
     9481 ggccacggcc gccatcaccc tgctggtgct gttctgcttc aaccaggtcg gcgatggtta
     9541 tgggctgttt atcccacgct tggtcgacac cttactcggc agcctgattg ccggcctggc
     9601 ggtgctgctg attctgccgg actggcaggg tcgccgcctg aatcgcctgc tcgccagcac
     9661 cctgagctgc aacagccgct acctgcagca gatcatcgcc cagtacgcca gtggcaagcg
     9721 cgacgacctg gcctatcgcc tggcccggcg caatgcgcac aacgccgacg ccacgctgtc
     9781 gaccaccctg ggcaatatgc tcatggaacc gggacacttt cgtaaggaag cggatatcgg
     9841 ctttcgcttt ttggtgctct ctcacaccct cttgagttac ctctcgggcc ttggcgcgca
     9901 ccgtggcgaa accagcctga gcggcaacga caatccgctg ctgaccagcg ccagccagtt
     9961 ggccgccagc ctggaagaga ttgccagctg cctgaacgag gagcgcccgg tggccatcca
    10021 cagtgacgcc gaagagcaac tggcagccga gcttgaacaa ctcagcgacg acctggacga
    10081 ccagcaacgc ctggtgcaaa cccagctggc gctaatctgc cgccaactcg ggccgctgcg
    10141 caccctggcc gcacatctac tgaagagcca cgcatgaaca aatcccagtt gctgcaacag
    10201 gttatcgccc gcctcgaaga tgacctgctg caggcccaac aggcggcgct aaccgcctat
    10261 gaaacggcga cccatgaaga aaacatcgcc gaaaacaaat acgacaccct gggcctggag
    10321 gcgtcctacc tggccaccgg gcaggcacgc cgcgcggccg ccattcgcca gaccctggcg
    10381 aactggaaac aactggtgtt gcgtggcttc gacccacaac tgggcattca acttggcgcg
    10441 ctgatcgagc taagcgatga ccaagaccgc cgccaacgcc tgttccttgg cccggacggg
    10501 gcggcgatga aactgcagca ggatggccag gccgtgcagg tcatcggccc gcaatcgccc
    10561 ctcgggcaag tgctgctcgg caagacaatc ggcgacgagg taaccctgcg cgtggccagc
    10621 ggtgtgcaac tgttagaaat tctatcggtc gactaacagg cggctgagaa atgccagagt
    10681 ccactcgacc ccggcagaat gaccgctacc ctctggtgca gaaacgcaag cccggctggc
    10741 tagcctgttg ggttgggtcc attgaagtcg agttcttcaa cggacggtta gaccgacgcg
    10801 ggctgcatgc taacaaagcc ctggctctcg ctgcgctcaa cgcaggcggc tgttctagag
    10861 ctggaaacgc gagatattgt cacgtagcgc cacactggtg cgtgacaggc tttcgctttc
    10921 ctgctgagcg ctacggaagg cagctgcgga ttgttcggag ctatcgcgta cggtaacgat
    10981 gccccggctg atctgttcgg caacggtgct ctgctcctcg gaagccgctg caatctgctg
    11041 gttcatgcca gtgatccgct caatcatgct gacgatgcgg gtcaccgctt cgccggcctc
    11101 ggaaacacca gaaaccgact gctcgctgga gtgcttgcaa cgatgcatgt gctgtgaggt
    11161 ttcatcagcg atcctctgca ggccggcgat cagcccctcg atctccgttg tcgcctcctg
    11221 ggtacgccgc gccaggctgc gcacctcatc cgcaaccacg gcaaaaccac gaccggcatc
    11281 gccggcgcgg gcggcctcaa tggcagcatt gagcgcgagc aggttggtct gatcggccac
    11341 cgacttgatc acatcgagta cgctgccaat gttgctgctc tcgtttttca ggcgctccat
    11401 ggccacatta gactgctcaa tcgtctcagc cagttcatca atcaggctaa cggcctggct
    11461 ggccttctgc tggctgtgca gggccgcgcg atctgcttct tgggccgcac cagcggcggc
    11521 ctcggcattg cgcgcaacat cctgcacggt ggcagccatt tgatggatgg cggtgacgat
    11581 ctgctcgacc tcaagctgtg cctggttcac gctttcggcg ttacgcaggc tcgactgact
    11641 cagctcctga ctggattggg ccacgatctg agatgagtgt tcgatgcctt gggtcatctc
    11701 ccgcaggttc tgctgcatgc gctgtagggc cgccagtaca ctgtctttgg gcggttcgct
    11761 gttgcgctca ccaacacgta aatcaccctc ggcaacccgc tgggcgagtt ggttgagttc
    11821 actcggctcc gcacccagtg cgcgcagcag attacggctg atccagtggc ccagtacggc
    11881 tgccagcaca aacgccagag cggtgatgat gatcagcaga ttgcgctgct ggtgatagtg
    11941 caggtcatta gcaacaacta actgatccgc catcttgttg gtgttgttca ggtattcact
    12001 gatgacctgg gtcagctcct ccagcaacgg tgtgcattgc tcgctaacca tgcgcagggc
    12061 ttgggcgtct tccccgttga acaggtgctc agcaatagcc tgggcaaccg ggctgtaacg
    12121 cgactcgacc tgctcgatac gcgaaaacag ctcacgtccc gcagccgata caccgtcttg
    12181 tgtcgtaacc gcctggcgta aggcggcgag gctgttagcc acggttttct catgggcttc
    12241 gatggcggtg cgctgttgtc cgcgggtagc cgcattgcca ttgagggcca ggttacgcag
    12301 ggcaatggcg cgatccttgg cggcgttgct tagctcgttg gctaagcggg cgcggttacc
    12361 tacgccgttc acgtagtctg agaaaccttt gttgctatca gccaagttgt aaagcgagac
    12421 aatggcgatc acgagcatga gggcaagcag gctggcgtaa ccggcataga gcgtagtgcg
    12481 ggtagaaagg cgcggggcat gtgagggcat atgtactacc tattgcgtca cgaggacgac
    12541 tcgatcgtgg ctggtttgca caccaataag gatagaccgg tgtctttaaa atgcatcctg
    12601 cacgggtgtg tttgagtcgt tcttccgcga acggctgttg cttactggat aaggcttttc
    12661 agctcaagct atacaacaac gtaacaatta tgtccgcctt gtataagatt gcaatgcgac
    12721 taatgattgg ctcatatgac cgacgagtca taaaaaacgg atggtactca agaacttgtg
    12781 gccgcctacc tggctttggc aggcgactaa aatagtgctg cagtggttac caatacacac
    12841 gccttttgtg cggttatgag tagcaccaat accgctgcct agttcaatag agcgctgaga
    12901 gcaccgtcag gagccaactg aagcgccgct gggacagacc ctacgtggca caccgaagtg
    12961 accctgtatg tggtcagcag tgtgcaactg ttggagattg tgcaggtcgc tcaaggaata
    13021 tccacttgcc agtctgcagc caactgctcc agccggcctt gctcgaccat ctgctgcagt
    13081 gctcggtcga acgcctcacg taaaggcccg aaacgctcat cgcggggaaa ggcgaagtaa
    13141 ccgtcctgca gatcgatcgg cggctccagt tgctcgtgtt tatcaaccag ccccaacgcc
    13201 aaaagcaccg actgggtatt gcgctgattg ctggccaata aatccagacg accggcaccg
    13261 agcattttca gaccattctc tacccgttcc acagtgacca actcgagctg cgcgcgctgg
    13321 ctgtcgaaat gtgcaccata gaaccaccca cgcaccacac caatgcgccg accgaacagc
    13381 tgctgatagt cgccgtgcca gcgcaacccg ctgccgcgct ggcggtagaa gacgatgtgg
    13441 tcgcggtaaa agggcagagc ggaaaaggcg aaaagttgct cacgcgcggc gttcttgtag
    13501 ggcccgataa ggatatcggc ctccccgcgt gccaccatgt gctgcgcacg ggcccaggga
    13561 tgcagctcaa agcgcacctg atagcccagc ggctccagcg cctggcgcaa tacactggta
    13621 gcgaggccgc tatagccgct gccgttctgc tcgaacacac caggaaaatt gctgcctaca
    13681 gccaacagct cgcgcgctgc caagggtgcg atcagcagca cacagcaaca tgccaacgac
    13741 agcctgcgca gaaagttcga acacatgggt agcggcctca gccacaggcg ggtggcgata
    13801 cttgccagcc tagcaggtca gtcgggtacg caagcggagc aacgcaccgt catgcaactg
    13861 ttggagattc tatcggtcga ctaagaagac catcaatcga tggccataag gggagacctt
    13921 catgcaaagc aacactccag agctcgaaac actgttccag caactgggtc tcgagcatga
    13981 cccgatcagt atcgacctct ttatcaaacg ccattcgccc ctagcggaag actgcctgct
    14041 ggcggacgcg ccgttctggg acaaggccca ggcagacttt ctccgccaac agctgctggc
    14101 tgacgccgaa tgggccgaag tcgtggatca gctcaaccta ctgctgcggg gctgacaact
    14161 caccaagcga gccaggcagt tcgcgccagg tgaggtaaac ccgcagatcg aactccaact
    14221 ggtggtagcc gggcagcatg tactcacaga gcttgtagaa ggccttgttg tgctcagcct
    14281 ccttcaaatg cgccagctcg tgcaccacga tcatctgcag aaactccggc gcagcctcgc
    14341 gaaacagcga ggccacgcgg atttccttct tcgccttgag cttgccgccc tgcacccgcg
    14401 agatcgccgt atgcaggccg agggcgcggt gggtcaggtc gaggcgattg tcgtagagca
    14461 ccttgtcgat ggccggggca ttacgcaggt gctcctgctt aaggccctgg atataggcat
    14521 ataacgcctt gtcactttga atcgcgtgct tgccgctgta ggaccgcgcc agataatcgc
    14581 ccaggcgctg ctcggcgatc agctggcgga tcttgtcttg cagctgggcc gggtagcctt
    14641 gcaggtattt aagcggggtc atgatcgaag tgacgcggca cgatagggcc gtggattatg
    14701 ccaaaagccg cacgcaccag ggtgcggctt ttggtggtgc aattattgac tcggcaccac
    14761 ccgcagcagc cgaccatcag cactgtcggt aagcaggtac agcgcgccat ccgggccgcc
    14821 gcgcacgtcg cggatacgct cctccaactc ttcgaacaac acttcttcac cggccagctt
    14881 gccttcctgc aggcgaatgc ggcgcacgct gcgttcggcc agggtcgcgg caaacaggtc
    14941 gccctgccac tcggggaaca gcgcgccgcg gtattgagtc aggctcgacg gcgccaccga
    15001 gggcgtccat tgcaacaagg gctgctgcaa accgggcagt tcggtgtagg gcgagatttg
    15061 cgcaccggtg taatcgacgc cgaaggtaat cagcggccag ccatagttgt tgccgggttc
    15121 gatcaggttc agctcgtcgc cgccgcgtgg gccgtgctcg tggctgtaca ggcgctgcag
    15181 ctgattgtca tagacgatgc cctgcacgtt gcgatggccc aggctgtaga tctctgcagc
    15241 ggcgccggcc tggcccacca gcgggttgtc cttgggaatg ctgccgtcgc ggttgaggcg
    15301 cacgattttg ccgatatggt tagtcggatc ctgggcctgc tcacgcaggt caaagccatc
    15361 gcccagggtc aacaccaagg tgttatccgg cagccaggcc atgcgcccgc cgtagtgcgc
    15421 ggcgccgctt ttggccggca gcgcgctgaa cagcacctgg acatcctgca gctggccatc
    15481 gaccagccgc gccttggcca gacgggtgtt gttggcctgt gcggtgccat aggcgtaact
    15541 cagatagagc tgctgattgc tggcgaactc agggtcaagc agcacctcca tcagcccggc
    15601 ctgagcggcg acaaatgcct caggcacgcc gccgaccggc tgcggggtga ggctgccgtc
    15661 ctgctcgatg atgcgcaggc ggccggcccg ctcggtcacc agcatgcgcc catccggcag
    15721 aaaggccagc gaccagggat gctccaagcc ttcggtcacg gtttcgatgc ggtaatccat
    15781 ggcgtgcgcc aggctgctgc caagcagacc aagcaacaac agggcgcggg aggtattcaa
    15841 catgattcgg gcctttgggt tgcagagggt tatcaggatt gcgcgtgcgg cacacgccgc
    15901 gccaacaggg caaacaacag cgcgcccagg ctgccgcaga ccatcagccc gagggtgccg
    15961 gccaggctgc ggttagcgcc gatcaaggtt ggcatcggca gcaacgcatt ggccagagcc
    16021 atcgccgcca acaccgccag cgcaccggcc agggtaaaca gcagccagcg ccacgtcggc
    16081 caagcgcgcg ccaaccggct cgcagccggc aaggcaaaga taaagccgag aatcaccagc
    16141 accgcgaagg ttgggctaaa gcccagcagg tccaggcagg tggtctgcac ccgcacgcca
    16201 agcggcatgg gcgcgccgag tgcctgcagg ttggccaggt tgaactgggt ctgcagcacg
    16261 ctgcccatca cactcgccag cagcacagcc agaaagaagt acaacagagt gcgcaatttg
    16321 ctcggcatgc agacagatat ccagagacga cggtggcccg agtatggacc agcgcgccgc
    16381 gcggtacgtg gccggtgttt tacatggttt taccaagccc acaaatgggc gataggcgcc
    16441 gtcactgtgc tgtagcagaa tgcccgcagc ctccagccga taccaatccg cataagaacc
    16501 gcacgatgaa aaaagccctg cccttctctg cgctgatcat cgcgctgctc gccatagcat
    16561 tcagcctcgg tcccgtgccg ccagtcgata cacaactgac gccgacccag ctgcctgccg
    16621 acctcgagcg ttatctggcc gagaccgaag cgcgctaccc ggacattacc cctggcgcag
    16681 aaaagaccat cgtctgggcc cattcggacc aacgtcaaac cgatttagcg atcatctacc
    16741 tgcacggtta ttcggccacc cgccaggaaa ccgcgccgct cagcgaccag ttggcccagc
    16801 aactgggtgc caatctgttc tccacccgcc tgagcggtca cggccgcagc ggcacggcga
    16861 tggccgaagc cagcgtgcac gactggctgc aggacagtca ggaggcactg cagatcggcc
    16921 agcgtctggg caaacaggtg ctggtgatcg gcacctcgac cggcgccacc ctcgccacct
    16981 ggctggcgct gcaaagcgat aacccacagg tgctggccta tatcatggtg tcgccgaact
    17041 tcgcccccaa ggacccggcc gccaccgtgc tgacctggcc ctgggccaaa catttcgtgc
    17101 cgttgctgct gggcccggaa catcattggc agccgcgcaa cgccgagcag gcgcgctact
    17161 ggagccatcg ctacccggtg caggcgctgc taccgatgat ggcgctggtg aaatacgtgc
    17221 gtgagcagcc gctggagcag atcaacgcgc caattctcac gctctactcg aaggacgatc
    17281 aggtggtcag cgctccagcc atcgaagccg catttgcccg cttcagctcg gcgcataagc
    17341 aacttattgc cctcaaggac acccaggatc catcccacca tgtgctggcc ggcgacattc
    17401 tttcgccgaa tgacacgcca cgggtgcaag cggcgattct ggcgtttttg gccaacttgc
    17461 cgccgcgcta gtcgaaacga cacagccggc ctgaagggca caaagaacca gcgcaagtag
    17521 cgtgctgatg gccgaacgcc tgcagctcat cgcctgtagc agcctggcgg cgcggcgaga
    17581 tgccagacgc agaccaacca ctcgtcggat agctcaatgc gggaaagtgc tcggcggggc
    17641 tgctcttcca aaaaaatgac gtttctcgca aaagactttc cttaattatc aaggcgcaaa
    17701 tttttcccga gcattttcca ggctgtctat cgtgctggcc aagcactggc caggcacaaa
    17761 actagacatt ttattgacgc caataaaaca ctcagcctag ttaacacgac ccgcgatcac
    17821 gcgtataaag cctgcgactc gacggcctcc cgaaaacctc gagcgctgcg gcaagcctgc
    17881 cgctctttcc ctagccctaa cttttagttc gagcgcacag gccggaatgc cgagtaccta
    17941 gctactgact cgcggatgag atatgcccga ctgaaagttc tctatcgaga actcagtcat
    18001 gattgacctt gcaacctgga acctcaccat tcccgtcggc gtaccagcag caaccatctc
    18061 aaccgagctg cttgtaggcg gctatcagga ccactacttc caatccaagg aagccagcat
    18121 ctttttctgg gcgccagtca acggcacagc caccagcaat gccagctacc cgcgcagcga
    18181 actgcgcgaa acctttgccg acggcaaact gcgtaattgg acctaccccg aagcggacca
    18241 ctttctgcgc gccgcgctga aggtcagcca ggtgccctcc actggcaaac tggtgatcgg
    18301 ccagatccat gcccacaaga gcaccatgcc cttgctcaag ctggagtatc aatacaaggc
    18361 ttattccgct agcggcaata tcgtcgccaa ggtgcgcatc gcgcccgacg cgccgattca
    18421 ggtcgttacc ctggccaatg gcattccatt gaaccagcgc ttcacctatg tgctgcacct
    18481 gacgccgaag ggcacgctgg cgatcaacat caacggggtc aagtggagca ctcggctcga
    18541 ctcggtctgg gccagcaaac cgctgtattt caaagcgggg gtctataccc aggacaacac
    18601 cggctatgaa accgaagccg gcgctgcgcg cttcgataag ctgagcatcg agcaccgccc
    18661 cctgcccact accgcaccgt aaggcgcgcc tcgcgcataa aaaacgccgc ggcacctttt
    18721 gggtgtcgcg gcgttttctt tagcgagcgg ctaacgcggc ggcctgttcg gcgcgcagca
    18781 ccggccgcct ggatcagttg accttggcgg ccagctcacc cttggcataa cgttggaaca
    18841 tgccttccag ggagatcggc ttgatcttcg aggcattgcc ggcggtgccg aaggcttcgt
    18901 agcgggcgat gcagatatcg cgcatggcgg tgacggtggc accgaagaat ttgcgcgggt
    18961 cgaactcgct cgggttggtg gccatcaggc gacgcatggc accggtggac gccaggcgca
    19021 gatcggtgtc gatattgacc ttgcgcacgc cgtacttgat gccttcgacg atttcttcga
    19081 ccggcacacc gtaggtttct ttgatgtcgc cgccgtactg gttgatgatc gccagccact
    19141 cttgcggaac cgaagaagaa ccgtgcatca ccaggtgggt gttggggatg cgcttgtgga
    19201 tttccttgat gcggtcaatg gcgaggatgt caccggtcgg cggc
//
LOCUS       DASRUM010000094        10857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1394241, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000094 DASRUM010000000
VERSION     DASRUM010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 10857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10857
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394241"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..1054
                     /locus_tag="VFF22_06435"
     CDS             20..1054
                     /locus_tag="VFF22_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HZX16488.1"
                     /translation="MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDH
                     KQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVARFRVNAFNQNRGSGAVFRTIPSK
                     VLSMEDLGMGEIFRKISDVPRGLVLVTGPTGSGKSTTLAAMLDYINSNKYHHILTVED
                     PIEFVHESKKCLINQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAA
                     ETGHLVFGTLHTTSAAKTIDRVVDVFPAEEKSMVRSMLSESLQSVISQTLMKKIGGGR
                     VAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDSCLKTLVSKGIVSREVA
                     REKAKTPENF"
     gene            1160..2305
                     /locus_tag="VFF22_06440"
     CDS             1160..2305
                     /locus_tag="VFF22_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HZX16489.1"
                     /translation="MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPITKTPMSP
                     EHTRETVHAVMNEQQRRDFTENHECNFAISARGVGRFRVSAFYQRNLAGMVLRRIETN
                     IPTLDELKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
                     PIEYIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFA
                     ETGHLCLATLHANNANQALDRIINFFPADRQNQVLMDLSLNLKAIVAQQLIPTPDGKG
                     RRAVIEVLINTPLAADLIRKGDVHELKALMKRSTELGMQTFDQALYNLYSQGEITYED
                     ALLYADSANDLRLMIKLGSETDGDHLSSMAQGLSLEVSEEDPGRRFR"
     gene            complement(2271..3149)
                     /locus_tag="VFF22_06445"
     CDS             complement(2271..3149)
                     /locus_tag="VFF22_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HZX16490.1"
                     /translation="MHSTPFDGSFAGWRDAARRLLQQGVPPHQITWLTDGDSGGLFDH
                     APAPSSPPVAKPPLRIPKQLLELLQNAARFRVEDRWSLLYRILWRVAQGDRAAMLPGD
                     IDGSQLHKRLKAVRREAHHLHAFLRFHPRPEHAEGPQLVAWHEPAHDILASASGHFAE
                     RLGRSTWLIATPDDGVYWDGQRLHYQRPCPSVWQRLALAPEDEGEALWLAYYGSTFNP
                     ARLNRSALETSMPVRFWKNRPEGPLIPRLMSQARAGAQRDGQAEAVARQGGKRIRRTS
                     AKPPAVSGSADPDLLR"
     gene            complement(3149..4360)
                     /locus_tag="VFF22_06450"
                     /pseudo
     CDS             complement(3149..4360)
                     /locus_tag="VFF22_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818748.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
     gene            4518..4916
                     /locus_tag="VFF22_06455"
     CDS             4518..4916
                     /locus_tag="VFF22_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HZX16491.1"
                     /translation="MQRQDTHSKLIGYLLWIFGFLGAHRFYYGKPVTGTIWFFTFGLL
                     FIGWIVDLFLIPAMDREADLRFTAGDTDYNVAWILLTFLGIFGVHRMYQGKWITGIIY
                     LFTGGLFLLGVLYDFWTLNTQVSIKNAERA"
     gene            complement(4972..6243)
                     /locus_tag="VFF22_06460"
     CDS             complement(4972..6243)
                     /locus_tag="VFF22_06460"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZX16492.1"
                     /translation="MRIAILGARAIDPSSGFDQLADIYIEGGKIAAIGQAPAGFVAEQ
                     SIDAQGLVAAPGLVDLNVALREPGYSRKGSIATETLAAAAGGVTSLCCPPFTKPVLDT
                     PAVAELILDRAREAGHTKVFPIGALSKGLEGEQLAELVALRDAGCVAFGNGLDSFRNN
                     RTLRRALEYAATFDLTVIFHSQDHDLAEGGLAHEGATASFLGLPGIPESAETVALARD
                     LLLVEQTGVRAHFSQLTSARGAQLIADAQARGLPVTADVALYQLILTDEALSDFSSLY
                     HVQPPLRTANDRDGLRAALKNGVISAISSHHQPHERDAKLAPFGATEPGISSVQLLLP
                     LAMSLVQDGLLDLPTLLARLSSGPAQALRLPAGNLSVGAPADLLLFDPQSSTLAGESW
                     YSKGANCPFIGHCLPSAVRYTLVDGHVSYKA"
     gene            complement(6243..7247)
                     /locus_tag="VFF22_06465"
                     /pseudo
     CDS             complement(6243..7247)
                     /locus_tag="VFF22_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678427.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
     gene            complement(7258..7764)
                     /gene="pyrR"
                     /locus_tag="VFF22_06470"
     CDS             complement(7258..7764)
                     /gene="pyrR"
                     /locus_tag="VFF22_06470"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HZX16493.1"
                     /translation="MSLPNPAELLPQMAAALTSHLNQRQISEPRFIGIRTGGVWVAQA
                     LLEALGRDDALGILDVSFYRDDFTQNGLHPQVQPSELPFEIEGQHLVLIDDVLMSGRT
                     IRAALNELFDYGRPASVTLVSLLDLNARELPVRPDVIGATLSLAADQRVKLSGPTPLT
                     LELQTLAN"
     gene            complement(7796..8236)
                     /gene="ruvX"
                     /locus_tag="VFF22_06475"
     CDS             complement(7796..8236)
                     /gene="ruvX"
                     /locus_tag="VFF22_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZX16494.1"
                     /translation="MAENAKPLRLLLGFDYGSKQIGVAVGQVITGQARELCILKAQNG
                     VPDWQKVEALIKEWQPDAIVVGLPLNMDGTKSEMSDRAEKFARRLNGRFNLPVHTHDE
                     RLTTYEAKGQRLQQGQNSGYRERPVDALAAALLLQGWLEENCLG"
     gene            complement(8236..8805)
                     /locus_tag="VFF22_06480"
     CDS             complement(8236..8805)
                     /locus_tag="VFF22_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HZX16495.1"
                     /translation="MKDASPSYLKHHFLIAMPHMADPHFAQTVTYIIDHNEHGAMGLV
                     INKPNGLNLADVLEQLRPDDEPPVLCQSLPIFTGGPVQTDRGFVLHPSGPQFQATLEL
                     GELGLSTSQDVLFAIADGTGPEKYLITLGYAGWEAGQLEAELADNAWLTCPADNAILF
                     DLPFDQRLNAAAARLGVNLSLLTSQAGHA"
     gene            complement(8878..9774)
                     /locus_tag="VFF22_06485"
                     /pseudo
     CDS             complement(8878..9774)
                     /locus_tag="VFF22_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713610.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
     gene            complement(9831..10793)
                     /gene="gshB"
                     /locus_tag="VFF22_06490"
     CDS             complement(9831..10793)
                     /gene="gshB"
                     /locus_tag="VFF22_06490"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HZX16496.1"
                     /translation="MSVRLGIVMDPIAQIAFKKDSSLAMLLAAQARGWTLFYMEQKDL
                     YQAKGEARARLSELSVYNDPQRWFELGNEHDIALSELDVILMRKDPPFDNEFVYSTYL
                     LEQAEQAGVLVVNRPQSLRDCNEKYFATQFGQYTPPTVVSRRADILREFAKEQRDIIL
                     KPLDGMGGSMIFRHREGDPNLSVILEALTNHGQQQIMAQGYLPAIKDGDKRILMIDGE
                     PVPYCLARIPAAGETRGNLAAGGRGEARPLTERDREIAAAVGPTLREKGLLFVGLDVI
                     GEHLTEINVTSPTCIREIDAAFDIRIGEQLMDKIAEKLNARSAS"
BASE COUNT         1933 a         3150 c         3587 g         2187 t
ORIGIN      
        1 tctgtcatta gggagtccca tggatatcac tgaattgctg gccttcagcg ccaagcaagg
       61 tgcatcggac ctgcatctct ccgctggcct gcctccgatg attcgggtcg acggtgatgt
      121 tcgccggatc aatttgcctg cactggacca taaacaggtg cacgcactga tctacgacat
      181 catgaacgac aagcagcgca aggatttcga ggagttcctc gaaactgact tctccttcga
      241 agtacccggc gtggcacgtt ttcgggtaaa cgccttcaac cagaaccgtg gttctggcgc
      301 ggtattccgt accattccgt cgaaagtgct gagcatggaa gacctcggca tgggcgagat
      361 cttccgcaag atatccgatg tgccgcgcgg cctggtgctg gtgaccgggc cgaccggttc
      421 gggcaagtcg accaccttgg cggcgatgct cgactatata aacagcaaca agtaccacca
      481 catcctcacc gttgaggacc cgatcgaatt cgtccacgaa tcgaagaagt gcctgatcaa
      541 ccagcgtgag gtgcaccgcg acaccctcgg cttctccgaa gccctgcgtt cggcgctgcg
      601 ggaagacccg gacatcatcc tggtcggcga gatgcgcgac ctggaaacca tccgcctggc
      661 cctgacggcc gcagaaaccg gtcacttggt gttcggcacc ctgcacacca cctctgcggc
      721 caaaaccatt gaccgcgtgg ttgacgtatt ccccgccgaa gagaaatcca tggttcgttc
      781 catgctctcc gagtccctgc aatcggttat ctcgcagacc ctgatgaaga agatcggtgg
      841 cggtcgcgtg gccgcccacg aaatcatgat cggcaccccg gccatccgta acctgatccg
      901 cgaggacaag gtggcgcaga tgtactcggc gatccagact ggtggctcgc tgggcatgca
      961 gaccctcgat tcctgcctga aaaccctggt atccaagggt atcgtcagcc gcgaagtcgc
     1021 gcgcgaaaag gctaagaccc cggaaaactt ttaaaccgac agggctcggc aggcgtcgcc
     1081 tgtctggttc agcccagcgg caatccacgc gcctgccgct ggccttgcat aaccgcgtga
     1141 tgcacatggg cgaacaacga tggaattcga aaaactgctg cgcctgatgg tggaaaaggg
     1201 cggctctgac ctgtttatta ccgctggcgt gccaccgtcg atgaaggtca acggcaagat
     1261 catgccgatc accaaaacgc ccatgtcgcc ggagcatacc cgtgaaactg tgcatgcggt
     1321 gatgaacgaa cagcagcgtc gcgactttac cgaaaatcac gaatgcaact tcgccatcag
     1381 cgcccgtggc gttggccgtt tccgtgtcag tgccttctac cagcgcaacc tggcgggcat
     1441 ggtgctgcgc cgtatcgaga ccaatatccc cacgctggac gagctgaaac tgccggaaat
     1501 tctcaagaag ctggccctga ccaaacgtgg tttggtgctg tttgtcggtg cgaccggtac
     1561 cggtaagtcg acttccctgg cggcaatgat cggctatcgc aacaagaaca gcagcggcca
     1621 catcatctcc atcgaagatc ccatcgagta tatccaccag caccagagct gcatcgtcac
     1681 tcagcgcgaa gtgggcatcg acaccgagtc cttcgaagtg gcactgaaga acaccctgcg
     1741 ccaggcgccg gacgtgattc tgatcggtga agtgcgcact cgcgagacca tggaccatgc
     1801 tgtggccttc gccgaaaccg gccacctgtg cctggccact ctgcacgcca acaacgccaa
     1861 ccaggcgctg gaccgcatca tcaacttctt cccggcggac cggcagaacc aggtgttgat
     1921 ggacctgtcg ctcaacctca aggccattgt tgcccagcaa ctgatcccca ctccggatgg
     1981 caagggccgc cgtgcggtga tcgaggtgct gatcaatacc ccgctggctg ccgacctgat
     2041 ccgcaagggg gatgtgcatg agctcaaggc gctgatgaag cgctccaccg agctgggtat
     2101 gcagaccttc gaccaggcgc tctataacct ctacagccag ggcgaaatca cctacgaaga
     2161 cgcgctgctg tatgcggact cggccaatga cctgcgcctg atgatcaagc tcggttcgga
     2221 aaccgatggc gatcacctga gcagcatggc ccagggcttg tcgctggaag tcagcgaaga
     2281 agatccgggt cggcgcttcc gctgactgct ggcggtttcg cgctggtgcg gcgaatgcgt
     2341 ttgccgccct ggcgcgcaac cgcttccgcc tggccatcgc gttgcgcgcc ggcgcgggct
     2401 tggctcatca gccgtggaat cagcgggcct tccggccggt ttttccagaa acgcaccggc
     2461 atgctggttt ccagggcgct gcgatttagt cgcgccgggt tgaaggtgct gccgtaatag
     2521 gccagccaca gagcctcccc ctcatcctcg ggcgctagcg ccaggcgttg ccatacgctc
     2581 gggcaaggcc gctggtagtg caggcgctgg ccatcccaat acacgccgtc atccggtgtg
     2641 gcaatcaacc aggtactgcg ccccaggcgt tcggcaaagt gcccgctggc actggcaagg
     2701 atgtcatggg caggctcatg ccaggccacc agctgtggcc cttcggcgtg ttccgggcgc
     2761 ggatgaaagc gcagaaaggc atgcaggtgg tgggcttcgc ggcgcaccgc tttgagccgt
     2821 ttatgcagct ggctgccgtc gatatcccct ggcagcatgg cggcgcgatc accctgggcg
     2881 acgcgccaga gaatccggta gagcaggctc cagcgatcct ctacacgaaa gcgtgcagcg
     2941 ttctgcagca gttcaagcag ctgcttgggg atgcgtagtg gcggtttcgc tactggtggg
     3001 ctgctgggtg ccggagcgtg gtcgaacagc ccgccactgt cgccgtcagt cagccaggtg
     3061 atctgatgcg gtggcacgcc ttgctgcaac agccttctgg cggcatcgcg ccagccggca
     3121 aagctgccgt cgaacggcgt gctatgcatc accacagcgc catttgcgcg ggcgtttcgc
     3181 tgagctgttg gcgtagcagc agggattcgc ggctggcctg cagggggcgg taatcctggg
     3241 tgatgataaa tggcttggct ttttccagcg cgcagcgcag gcgcgtgagg tcgtcgaaac
     3301 gtatgcgttt ctgccgacgc agggccacca ggcgtttcgc gctgagcacg ccgataccgg
     3361 gtacgcgggc gatcatcgag gcttcggcgc ggttgaggtc gaccgggaag tgctcgcggt
     3421 tattcagtgc ccaggccagt tttgggtcga tatccagggc caggttgccg gggccgctaa
     3481 gcagttcgcc gacctcaaaa ccgtagctgc gcatcaggaa atcggcctga tacaggcggt
     3541 gttcgcgcag caggggcggc gcttcgaagg gcacgatatt cgggctttgc gggattggac
     3601 tgaacgccga atagtagacc cgacgcaggc ggtagtcgcc gtagagcgag gccgcggtgt
     3661 gcaggatggt gctgtcatcg gtggcgtcgg cgccgatgat catctgcgtg ctctggccag
     3721 ccggggcgaa acgcggcgcc cgtatttctg cgcgggcttc gctctcgccc tgatgaatcg
     3781 agtgcatggc ctgcttgatg gttagtacct ttttctccgg tgccaggcgg atcaggctgc
     3841 tttaggtggg cagttcgata ttcacgctaa ggcgatcggc atagcgcccg gcttcggcga
     3901 tcagcagtgg gtcggcatcg gggatggtct tgaggtggat atagccgcgg aactcgtgct
     3961 cttcgcgcag cagcttggcc acgcggatca gctgctccat ggtgtagtcg gccgagcgga
     4021 tgattccaga gctgagaaac aacccgctga tgcagttgcg cttgtagaag tcgatggtca
     4081 gggtcaccac ttcttccggg gtaaaacggg cgcgcggcac gtcgctggag cggcggttga
     4141 cgcagtactg gcagtcatac aggcagaagt tggtcagtag gatcttcagc agcgccacgc
     4201 agcgcccgtc cggggtaaag ctgtggcaga tgcccatgcc gttggtcgag ccgatgccgc
     4261 tcttgccttt ggagctgcgg ttgggggcgc cgctgctggc gcaggaagcg tcgtacttgg
     4321 cggcgtcggc gaggacggtg agtttctcga tcagctgcat gatcttgcct gcttaactgg
     4381 ttatgtatac agtatttagc aatcaccggc aggcatcaag cgttgatatg gctgatcgca
     4441 tcgcggattt ccccgggcgg cgaaccttgc gtagagtgtg gcgtcaaagc gctatcaagc
     4501 catcaggagg cacacatatg cagcgacagg acacgcacag caaacttatc ggttatctgc
     4561 tgtggatttt cggttttctc ggcgcgcacc gtttctacta cggcaagccg gtgaccggga
     4621 ccatctggtt cttcaccttc ggtctgctgt ttatcggctg gatcgtcgac ctgtttctga
     4681 tcccggcgat ggatcgcgaa gcggatctgc gctttaccgc cggcgacacc gactacaacg
     4741 tggcctggat tctgctgacc ttcctcggca ttttcggtgt gcatcgtatg taccagggca
     4801 agtggattac cgggatcatc tatctgttta ccggtggcct attcctgctc ggcgtgctgt
     4861 atgacttctg gacgctgaac actcaggtgt cgatcaagaa cgccgagcgc gcctgaatct
     4921 caggcacgaa aaagcccgct gaattcagcg ggctttttat gcggcgatga atcaggcttt
     4981 gtagctgacg tgcccgtcta ccagggtgta gcgcacggcg ctgggcaggc agtgaccgat
     5041 aaacggacag ttggcgcctt tcgagtacca gctctcacca gccagggtcg aggactgtgg
     5101 atcaaacagc agcaggtcgg cgggtgcgcc cacgctcagg tttccagcag gcaggcgcag
     5161 ggcttgcgcg gggccgctgc tgaggcgggc gagcagggta ggcaggtcta gcaggccatc
     5221 ctgcaccagg ctcatggcca gcggcagcaa cagttgcacg ctgctgatgc ccggttcggt
     5281 ggccccgaac ggtgccagtt tggcatcgcg ctcatggggc tggtggtggc tggaaatggc
     5341 cgagatcacc ccgtttttta gcgcagcgcg caggccatcg cggtcgttag cggtgcgcag
     5401 cggcggttgt acgtgataga ggctagagaa atcactgagc gcttcatcag taagaatcag
     5461 ctgatacagc gccacgtcgg cggtgaccgg caggccacgg gcctgggcgt cggcgatcag
     5521 ctgggcaccg cgggcgctgg tcagttggct gaagtgggcg cgcacgccgg tctgctcgac
     5581 cagcagcagg tcacgggcca gggccacggt ttcggcgctt tccggaatgc ccggcaagcc
     5641 gaggaagctg gcggtggcac cttcatgggc caggccgcct tcggccagat catggtcctg
     5701 ggagtggaaa atcaccgtca ggtcgaaggt ggcggcgtat tccagggcgc ggcgcagggt
     5761 gcggttattg cggaagctgt ccaggccgtt gccgaaggcc acgcagccgg cgtcgcgcag
     5821 ggccaccagt tcggccagct gctcgccttc caggcctttg ctcagcgcgc caatcgggaa
     5881 caccttggta tgcccggctt cgcgggcgcg gtcgaggatc agctcggcca cggccggggt
     5941 gtctagcacc ggtttggtaa acggcgggca gcacaggctg gtgacgccgc cggcggctgc
     6001 ggccagggtt tccgtggcga tgctgccctt gcggctgtaa cccggctcgc gcagggcgac
     6061 gttaaggtcg accaggccag gggcggcgac caggccctgg gcgtcgatgc tctgttcggc
     6121 gacgaagccg gccggagcct gaccgatggc ggcaatcttg ccgccttcga tatagatatc
     6181 ggcaagctga tcgaaaccgc tgctggggtc gatagcgcgg gcaccgagga tcgcgatacg
     6241 catcagttct gctcctcggc gtcgaattgg cgttgggcgt tctgcccgct catggccatg
     6301 gacagtacgg ccatgcgcac ggcgatgccg taggtgacct gattgagaat caccgactgc
     6361 gggccgtcgg ccactgccga ttcgatctcc acgccgcggt tgatcggccc cgggtgcatg
     6421 accagcgcat ccggtttggc cagtttcagg cgctgttcag taaggccgaa caggcggtag
     6481 aactcgccct cgctgggcag caagccgccg gtcatacgct cacgctgcag gcgcagcatg
     6541 atcaccacgt cgacgtcctt gagacctgcg gtcatgtcgc tgtagaccgt gacgccgtat
     6601 tgctcgatgc cgaccggcag cagggttttc ggcgcgatta cgcggatatc cgggcaacca
     6661 agggttttca gcgcaagcat attcgagcgc gccacccgcg agtgtaggat gtcaccgacg
     6721 atggccaccg agaggttctc aaagctgccc ttgtgccggc ggatggtcag catgtcgagc
     6781 atgccctggg tcgggtgggc gtgacggccg tcgccgccgt tgatgatcgc caggttcggg
     6841 cacacatgtt cggcaataaa gtgcgcggcg ccagaatcag catggcgcac gacgaagatg
     6901 tcggcagcca tggcttcgag gttgcgcatg gtgtcgaaca gggtttcgcc cttgctggtc
     6961 tagctggtcg acacgttgag ggtgatcacg tcggcggaca ggcgctgggc cgccagctcg
     7021 aaggtggtgc gggtacgtgt ggagttctcg aagaacacgt tgcacacggt cttgccacgc
     7081 agcagcggga ctttcttcac cgcgcgggcg ccgacttcga ggaacgagtc ggcggtgtcg
     7141 aggatttccg tcagcagctc gcggggcagg ccgtcgaggg acaggaagtg gcgcagttgg
     7201 ccttggtcat tcagctgcag cgggcgcttg gcggctatgg tcgtcatctg gcgagtctca
     7261 attggcaagc gtctggagtt cgagggtcag cggcgtgggg ccggacaatt ttacccgttg
     7321 atccgccgcc agcgacaggg tcgcgccgat tacatccggg cgcaccggca gctcgcgggc
     7381 attcaggtca agcaggctga ccagggttac gctggccggg cggccgtaat cgaacagctc
     7441 attgagcgcg gcgcggatgg tgcgcccgct catcagcacg tcgtcgatga gcaccaggtg
     7501 ctggccttcg atttcgaacg gcagttcgga gggttgcacc tgcgggtgca gaccgttctg
     7561 ggtgaagtcg tcacggtaga aggacacatc gagaataccc agtgcgtcgt cgcggcccag
     7621 ggcttccagc agcgcctgag caacccagac gccgccagtg cgaatgccga taaagcgcgg
     7681 ttcgctgatc tgccgctgat tcaggtggct ggtcagggct gcggccattt gcggtagcag
     7741 ttcggccggg ttgggtaagc tcatggcaac actccaggtg cagtcttagc gggggttagc
     7801 ccaggcaatt ttcttcaagc caaccttgta gcaataaagc ggcggccagg gcgtcgaccg
     7861 ggcgctcgcg gtagccgctg ttctgccctt gctgcaggcg ctggcccttg gcctcgtagg
     7921 tagtcaggcg ttcgtcgtgg gtgtgtacgg gcaggttgaa gcggccgtta aggcggcggg
     7981 cgaatttttc ggcgcggtcg ctcatctcgc ttttcgttcc atccatgttc agtggcaggc
     8041 caacgacaat ggcgtccggc tgccattcct tgatcagcgc ctcgactttc tgccagtccg
     8101 gcacaccgtt ctgcgccttg aggatgcaca actcgcgggc ctggccggtg atcacctggc
     8161 cgaccgcaac gccgatctgt ttactgccgt aatcgaaacc cagcagcagg cgcagcggtt
     8221 ttgcattctc ggccatcagg cgtgcccggc ttgcgaggtg agcaagctga ggttgacgcc
     8281 caggcgcgcg gccgcggcgt tcaggcgctg gtcgaagggt aggtcgaaga ggatggcatt
     8341 gtcggccggg caggtgagcc aggcgttgtc ggccagttcg gcttccagct gcccagcttc
     8401 ccagccggca tagccgaggg taatcaggta tttctccggg ccggtgccgt cggcaatggc
     8461 gaatagcacg tcctgcgagg tcgacaggcc cagctcgccc aactcaaggg tggcctgaaa
     8521 ctgcgggcca ctggggtgta gaacaaagcc gcgatcggtc tgtacgggcc cgccggtaaa
     8581 gatcggcagg ctctggcaca gcacgggcgg ctcgtcatcc gggcgcaact gttcgagtac
     8641 atcagccaga ttgaggccgt tgggcttgtt gatcaccagg cccatggcgc cgtgttcatt
     8701 gtgatcaatg atgtaggtaa ccgtctgtgc gaaatgcgga tcggccatgt gcggcatggc
     8761 gatcaggaag tggtgcttga ggtagctggg gctggcgtct ttcatgggcg ttagtttcag
     8821 gcttcaggcc attggcttca agcgcgacgc ggcatcgttg ggtaaaagtc tgcctgatca
     8881 gttgcttgac aggcggtcgc cgcgctcgaa gcgccaggtg cggatgattt ccaggcggtc
     8941 gacatcggcc aggtcgccgg tgaacggcgc gaacggcgcg gccaggcgca cgatgcgctg
     9001 ggcggcctgg tcgagggtgc gctggccaga cgattccagg acctgcacct cgtacagcgt
     9061 gccatcgcga ttgatcgaca ccagcagccg taggctgccg tagatgccct ggcggcgtgc
     9121 ttcgtccggg tagttgaggt taccgacccg ctcgaccttc ttgcgccact catccttata
     9181 ccaggcgcct ttgtcgcgca tggtcgaggc ggcgttgagg cggtggatct tcggccgttt
     9241 ggcgtattgc tgaacttact gggccagttc ggcttccagg ctggcgattt ccgaagacag
     9301 ttgcgcgctg tcgaataccg gtgctggtcg ggtttgcggt gtgggcttgg tttcttcgcg
     9361 tttgaccggg gttttttgct gttgcgggga gcgggtggcg acggccgcct tgggcgcttc
     9421 ctggcgcacg gccggctgtt gcggcgccgc gggcggagtg atgcgcttga cctcgctgtc
     9481 ctgaaacaga gcctgctcgg tggttttcgg cgcggccttg tggtccaggg tgccactgcc
     9541 ttgctggttg tcctgggcga ggaagtcggc ctctttgggc ttttcttcgc ttttgaaggt
     9601 ggagagggta atttccaggg ttttgctgat ctggcttggt tcggccaggg taaagcccag
     9661 gccgaggatc agcgcagcat gcagtacggc agcgaggaac agggtaaagc ccaggcggtc
     9721 cgccggctgt acgccggcgc tggaaagttc agggagttgg gctgctgcgt tcatcgcata
     9781 tcgggtctgc tcaggttgcc gcgcagtctg ccgagggcgc atggaaaagt ctatgaagca
     9841 ctgcgcgcat tgagcttctc ggcaatcttg tccatcagct gctcgccgat gcgtatatcg
     9901 aaggcggcat cgatctcgcg gatgcaggtc gggctggtca cgttgatctc ggtcaggtgt
     9961 tcgccgatca cgtccaggcc gacaaacagc aggcctttct cgcgtagagt cgggccgacg
    10021 gcggcggcga tttcacggtc gcgttcggtc agtgggcggg cttcaccgcg tccaccggcg
    10081 gcgagattgc cgcgggtttc accagccgcc ggaatgcgcg ccaggcaata aggcactggt
    10141 tcgccgtcaa tcatcagaat gcgtttatcg ccatccttga ttgcgggcag gtaaccctgg
    10201 gccatgatct gctgctggcc gtggttggtc agggcctcga gaatcaccga aaggttggga
    10261 tcgccttcgc ggtgacgaaa tatcatcgat ccgcccattc catcgagggg tttgagaatg
    10321 atgtcacgct gctctttggc aaactcgcgc agaatgtcgg ccctacggct gaccacggtc
    10381 ggcggagtgt attggccgaa ttgggtggca aagtactttt cattacagtc gcgcagactt
    10441 tgcgggcggt tgaccaccag aacgcccgct tgctcggctt gttccagcag gtaggtggag
    10501 tagacaaact cgttatcgaa gggcgggtcc ttgcgcatca ggatcacgtc cagttcgctt
    10561 agcgcgatat cgtgctcgtt acccagttcg aaccagcgtt gtgggtcgtt gtatacgcta
    10621 agctctgaca gtcgcgcgcg tgcttcacct ttggcctggt aaagatcttt ctgctccata
    10681 tagaagaggg tccagccgcg cgcttgggcg gccagtaaca tggccagcga gctgtccttc
    10741 ttgaaggcga tctgggcgat ggggtccatg acaatgccga ggcggacgct catggcggtg
    10801 ttctccgtaa aatggcagcg attaaagtgt gctaagacgt gatgtggcgt cagggtg
//
LOCUS       DASRUM010000095         6093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1884950, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000095 DASRUM010000000
VERSION     DASRUM010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884950"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..76)
                     /locus_tag="VFF22_06495"
     CDS             complement(<1..76)
                     /locus_tag="VFF22_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16497.1"
                     /translation="MSEVKHSRLIILGSGPAGYSAAVYA"
     gene            45..320
                     /locus_tag="VFF22_06500"
     CDS             45..320
                     /locus_tag="VFF22_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16498.1"
                     /translation="MIKRECLTSLIKTPHKPLSQKRMHAPIEPHGRCWRICYTEAGEE
                     RESTAVAVYAYSSVWYGAVKPVQSLHILPLTNYSAPLAQEQMRFEEI"
     gene            307..2709
                     /gene="ftsK"
                     /locus_tag="VFF22_06505"
     CDS             307..2709
                     /gene="ftsK"
                     /locus_tag="VFF22_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HZX16499.1"
                     /translation="MKKSSPTAQAPLWRQQLPYRLKEGALIGLGALCLYLWMALLTYD
                     AADPGWTHTSNVEQVQNAAGRAGAWFADILFMALGYFAYLFPLLLAVKTLQVFRARHQ
                     PWQWSGWLFSWRLIGLVFLVLSGAALAYIHFHASSSMPASAGGALGESLGALAINALN
                     VQGSTLLLIALFLFGLTVFTDLSWFKVMDLTGKITLDLIELIQSLANRWWSARVERKQ
                     LVAQLREVDERVSEVSAPIVLDRREQAKVKERLIEREESLNKHMSERVNRPAPVITPP
                     AAPKAPEPSKRVLKEKQVNLFEDSALAGSLPPISILDVAEKVQKKYSPEYLEAMSRLL
                     EIKLKEFGVEVIVESVHPGPVITRFEIQPAAGIKVSRISGLAKDLARSMAIISVRVVE
                     VIPGKTTVGIEIPNEDRQIVRFSEVLSSPEYDEAKSPVTLALGHDIGGKPVITDLAKM
                     PHLLVAGTTGSGKSVGVNAMILSILFKSTPEEARLIMIDPKMLELSIYEGIPHLLCPV
                     VTDMKEAANALRWSVAEMERRYKLMSKMGVRNLAGFNRKVKDAEEAGEPLSDPLYKRE
                     SMHDEAPLLKTLPTIVVVVDEFADMMMIVGKKVEELIARIAQKARAAGIHLILATQRP
                     SVDVITGLIKANIPTRMAFQVSSKIDSRTILDQGGAEQLLGHGDMLYMPPGTSLPIRV
                     HGAFVSDEEVHRVVEAWKQRGTPDYIDDILAGAEESGSGFEGGSGEGSEGSESDPLYD
                     EAVNFVLESRRASISAVQRKLKIGYNRAARMIEAMEMAGVVTSMNTNGSREVIAPSPV
                     RD"
     gene            2738..3364
                     /gene="lolA"
                     /locus_tag="VFF22_06510"
     CDS             2738..3364
                     /gene="lolA"
                     /locus_tag="VFF22_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HZX16500.1"
                     /translation="MRLIRMLLLTVLSFTSVAAMADDEVAVQRLTELLNQAQTINARF
                     SQLTLDGSGTQLQETAGQLALKRPGLFRWHTDAPMEQLLVSNGEKVWLYDPDLQQVTI
                     QTLDQRLTHTPALLLSGDVSKIRENFEISHKEGGNVVDFILKPKSKDTLFDSLRLSFR
                     NKVLNDMQLIDSIGQRTNILFLNVKMNEPQDDAQFTFEIPAGADVIQE"
     gene            3399..4724
                     /locus_tag="VFF22_06515"
     CDS             3399..4724
                     /locus_tag="VFF22_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013973284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HZX16501.1"
                     /translation="MDLFRKAPIAQPLAARLRATSLDEYVGQEHLLARGKPLREALEQ
                     GALHSMIFWGPPGVGKTTLARLLAQVSDAHFETISAVLSGVKEIRQAVEVAQQHAAQY
                     GRQTILFVDEVHRFNKSQQDAFLPYVEDGTLIFIGATTENPSFELNNALLSRARVYVL
                     KSLDESALRKLVDRALSDPKGLGERQLSLPEESFKILQAAADGDGRRLLNFLENAADL
                     AEDGSEIGSELLLNLLGDTRRRFDKGGEAFYDQISALHKSVRGSSPDGALYWFARMLD
                     GGCDPLYIARRVVRMASEDIGNADPRALTLCLNAWDVQERLGSPEGELAVAQAIVYLA
                     CAPKSNAVYNAFKAAMRDAAEHGSQEVPLHLRNAPTKLMKELGYGDEYRYAHDEPDAY
                     AAGEDYFPETFEPREYYQPVSRGLELKIRDKLQHLKNQDRNSPRQRRKP"
     gene            4721..5101
                     /gene="crcB"
                     /locus_tag="VFF22_06520"
     CDS             4721..5101
                     /gene="crcB"
                     /locus_tag="VFF22_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020307409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HZX16502.1"
                     /translation="MIRVAMAVALGGAVGSVLRFLTASWVVTQWPRHFYLGTFTVNIL
                     GCLLIGLLSGLFLLRSDLPIEFRSGLIIGLLGGFTTFSSFSLELLKLFESGRHNEAFA
                     YLLCSVVGGVLAVWGGFALARLYQ"
     gene            5206..>6093
                     /gene="serS"
                     /locus_tag="VFF22_06525"
     CDS             5206..>6093
                     /gene="serS"
                     /locus_tag="VFF22_06525"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZX16503.1"
                     /translation="MLDSKLVRTQLHEVAERLAARGFVLDVVRFEALEAQRKDVQTRT
                     EQLQAERNSRSKSIGQAKQRGEDIAPLLAEVDQMGSDLEQCKRDLDSIQTELDNLLLN
                     IPNLPHESVPVGADEEGNVEVSRWGAPRSFDFPIQDHVALGEQHGWLDFETAAKLSGA
                     RFALLRGPIARMHRALAQFMINLHTGEHGYEEAYTPYLVQAPALQGTGQLPKFEEDLF
                     KISREGEADLYLIPTAEVSLTNIVAGEILDPKQLPLKLVAHTPCFRSEAGASGRDTRG
                     MIRQHQFDKVEMVQIVEPSK"
BASE COUNT         1251 a         1798 c         1824 g         1220 t
ORIGIN      
        1 cagcgtaaac ggcagcgcta taaccggcag ggccggagcc caggatgatc aagcgtgaat
       61 gcttgacttc gctcataaaa acacctcata agcctttgtc acaaaagaga atgcatgctc
      121 caattgagcc gcacggaaga tgttggcgga tatgctacac cgaagcgggc gaagagcggg
      181 agtcaaccgc cgtggctgtt tatgcataca gctcggtctg gtatggtgcg gttaaacccg
      241 tacaatcgct gcatattttg ccgttaacca attattcggc gcctctggcg caggaacaga
      301 tgcgttttga agaaatctag cccaaccgca caagcccctc tctggcgtca gcagctgcct
      361 tatcgactga aagaaggcgc gctgattggc ctgggcgcac tttgccttta tctctggatg
      421 gctctgctga cctacgatgc ggccgatccg ggctggaccc acaccagcaa tgttgagcag
      481 gtacagaacg ctgccggccg cgccggtgcc tggtttgccg acattctgtt tatggcgctg
      541 ggctatttcg cctatctatt cccgctgctg ttggcggtaa aaaccctgca agtgttccgc
      601 gcccgccatc agccgtggca gtggagcggc tggctgttct cctggcggct gattggtctg
      661 gtttttctgg tgctgtccgg tgccgccctt gcctatatcc attttcacgc cagctcaagc
      721 atgccggcct ctgcaggtgg cgcgctaggt gaaagcctgg gggcgctggc gatcaatgcg
      781 ctgaatgtgc aaggcagtac gctgctgctg atcgcgctgt tcctcttcgg cttgacggtg
      841 tttaccgacc tgtcctggtt caaggtgatg gacctgaccg gcaagatcac cctcgacctg
      901 atcgagctga tccagagcct ggccaaccgc tggtggagtg cgcgggttga gcgcaagcag
      961 ctggtggcgc aattgcgtga agtcgatgag cgcgtcagtg aagtctccgc gccgatcgtg
     1021 ctggatcgcc gtgaacaggc caaggtgaaa gaacgcctga ttgagcgcga agagtcgtta
     1081 aacaagcaca tgagcgagcg ggtcaaccgc ccagcgccag tgatcacgcc gccagccgcg
     1141 ccgaaagcgc ctgaaccgag caagcgcgta ttgaaagaga agcaggtcaa cctgtttgag
     1201 gacagcgcct tggccggcag cctgccgccg atttcgatcc tcgatgtggc agagaaggtt
     1261 cagaagaaat actcgcccga atacctggaa gccatgtcgc gactgcttga gatcaagctc
     1321 aaggagtttg gcgtcgaggt tatcgtcgag tcggtccatc caggcccggt gatcacccgt
     1381 ttcgagatcc agcctgcagc agggatcaag gtcagccgca tttccggcct ggccaaggac
     1441 ctggcacgtt cgatggcgat catcagcgtg cgcgtggtgg aagtgattcc aggcaagacc
     1501 accgtcggta tcgaaatccc caacgaagac cggcagatcg tgcgcttctc cgaagtgctg
     1561 tcgtcgcccg agtatgacga agccaagtcg ccggtcaccc tggctctggg ccatgacatt
     1621 ggcggcaaac cggtaatcac cgacctggcg aagatgcctc acttgctggt ggccggtact
     1681 accggctccg gtaagtcggt cggggtgaac gcaatgatcc tgtcgattct gttcaagtcc
     1741 acgccggaag aggcgcgctt gatcatgatc gacccgaaaa tgcttgaact gtctatctac
     1801 gaaggtattc cgcacctgct gtgcccggtt gtgaccgaca tgaaggaagc cgccaacgct
     1861 ctgcgctggt cggtggccga gatggagcgc cgctacaaac tgatgtcgaa gatgggcgtg
     1921 cgcaacctgg ccggcttcaa ccgcaaggtc aaagacgccg aagaggccgg cgagccgctg
     1981 agcgatccgc tgtacaagcg cgaaagcatg catgacgaag cgccgctgct gaaaaccctg
     2041 ccgaccatcg tggtggtggt cgacgagttc gccgacatga tgatgatcgt cggcaaaaag
     2101 gttgaagagc tgattgcgcg tatcgcccag aaggctcgtg cggcgggtat acacctgatt
     2161 cttgcgaccc agcgcccgtc ggtggatgtg attaccggtc tgatcaaggc caacatcccg
     2221 acccgtatgg cgttccaggt atcgagcaag atcgactcgc gcaccattct tgatcagggt
     2281 ggtgccgagc aactgctggg ccacggtgac atgctctata tgccgccagg caccagtttg
     2341 ccgattcgcg tacacggtgc ctttgtttcc gacgaagaag tacaccgcgt ggttgaagct
     2401 tggaagcagc gtggcacgcc tgactatatc gatgacatcc tggccggcgc cgaagagtcc
     2461 ggtagcggct ttgagggtgg cagcggcgaa ggcagtgaag gcagcgagtc cgacccgctt
     2521 tacgatgagg cggtcaattt cgttctggaa agccgtcgcg cgtctatttc cgccgtgcag
     2581 cgcaagctga agatcggcta caaccgcgcc gcccggatga tcgaggccat ggaaatggct
     2641 ggcgtcgtca cctcaatgaa caccaatggc tctcgcgagg tcatagcacc aagccctgtt
     2701 cgcgattaat gcctagcccg atctgataag gagtcccatg cgcctgattc gcatgttgtt
     2761 actgaccgta ctgagtttta ccagcgttgc cgccatggcc gacgatgaag ttgcagtaca
     2821 gcgcctgacc gagttgctca atcaggcgca gaccataaac gcgcgctttt cccaactgac
     2881 cctggacggc agcggcaccc agctgcagga aactgcaggc cagctcgcgc taaaacgccc
     2941 aggcctgttc cgttggcaca ccgatgcgcc gatggagcag ttgctggttt ccaatggcga
     3001 gaaggtttgg ctgtatgacc cggatctgca gcaggtgacc atccagaccc tcgatcagcg
     3061 tctgacccat accccggcgc tgctgctgtc cggcgatgtg tcgaagatcc gtgagaactt
     3121 cgagatcagc cacaaagagg gtggcaacgt ggtcgacttt attctcaagc caaaatccaa
     3181 ggacaccctg ttcgacagcc tgcgtctgtc gttccgtaac aaggtgctca acgatatgca
     3241 gctaatcgac agcatcggcc agcgcaccaa cattcttttc ctcaacgtga aaatgaatga
     3301 accgcaggac gatgcgcagt tcaccttcga gatcccggcc ggcgcggacg tgattcaaga
     3361 gtagaacgct caacaacgag aggcgttaaa ccgcagtcat ggatctgttt cgcaaggctc
     3421 ccatcgctca acccctggct gcccggttac gcgcaaccag tctggatgag tacgtcggcc
     3481 aggagcactt gctggctcgc ggcaaaccct tgcgtgaagc gctggagcag ggcgcgctgc
     3541 actcgatgat cttctggggc ccgccggggg tgggcaaaac caccctggcg cggctgctcg
     3601 cgcaggtgtc cgatgcgcat ttcgagacga tttctgcggt gctctccggg gtcaaggaga
     3661 ttcgccaggc cgtggaagtg gcgcagcagc acgccgcgca atatggccgc cagaccatcc
     3721 tgtttgtcga tgaggtgcac cgtttcaaca agtcgcagca ggatgccttt ctgccgtatg
     3781 tggaagacgg cacgctgatc tttatcggcg ccaccacgga aaacccttcg ttcgagctaa
     3841 acaacgcctt gctctcccga gctcgcgtgt acgtgttgaa aagcctcgat gaaagcgcgc
     3901 tgcgtaagct ggtcgatcgc gccttgagcg atccaaaggg cctgggcgag cgtcagctga
     3961 gcctgccgga agaaagcttc aagattctcc aagccgccgc cgatggcgat ggccggcgtt
     4021 tgctcaactt tctggaaaac gccgccgacc tggccgaaga cggcagcgag atcggcagcg
     4081 aactgctgct caatctgctt ggcgataccc gtcggcgctt cgataaaggc ggcgaagcct
     4141 tttacgacca gatatctgcg ctgcacaaat ccgtacgcgg ctccagcccg gacggcgcgc
     4201 tgtactggtt tgcacgcatg ctcgatggcg gctgcgaccc gctgtatatc gctcgccgcg
     4261 tggtacgtat ggccagcgaa gacatcggca atgccgatcc gcgcgcgctg accctgtgcc
     4321 tcaacgcatg ggatgtacag gagcgcctgg gcagccctga gggtgagctg gcggtggccc
     4381 aggcaattgt ctacctggcc tgtgcgccga aaagcaacgc ggtctacaac gccttcaagg
     4441 ctgccatgcg tgacgccgcc gagcatggct cacaggaagt cccgctgcac ctgcgcaacg
     4501 cgccgaccaa gctgatgaaa gagttgggct atggcgatga ataccgctac gcccatgatg
     4561 agcctgatgc ctatgcggca ggcgaagatt actttccgga aaccttcgag ccacgcgagt
     4621 actaccagcc ggtgtcacgc ggcctggagc taaagattcg cgacaagctg cagcacttga
     4681 aaaaccagga ccgcaatagt cctcgccagc ggagaaagcc atgattcgcg tagccatggc
     4741 ggttgccctc ggtggtgcag tgggctctgt actgcgcttt cttaccgcaa gctgggtggt
     4801 gacgcagtgg cctcggcatt tctacctggg caccttcacc gtcaatatcc tcggctgcct
     4861 gttgatcggt ctgttgtcag gcctgtttct gctgcgcagc gatctgccaa tcgaatttcg
     4921 cagcggcctg atcatcgggc tgctcggcgg cttcaccacc ttttcctcat tcagccttga
     4981 gctgcttaag ctgtttgaaa gtggccgcca caatgaggcg tttgcctacc tgttgtgcag
     5041 cgtggtcggc ggcgtgctcg ctgtctgggg cggttttgcc ctggcgcgcc tctatcagta
     5101 gccgcgccag ctaacgtgcg gtctggcacc caaccctgtt aaagccttaa actgcgcgtc
     5161 atggccaagg ccatgcaaac ccttgtgatg tgttgagacc agaacatgct cgattccaaa
     5221 ctcgttcgta cccagctcca tgaagtggcc gagcgcctgg ccgcccgtgg ctttgtcctt
     5281 gatgttgtgc gtttcgaagc gcttgaagcc cagcgcaagg atgtgcagac ccgcaccgag
     5341 cagctgcagg ccgagcgcaa cagccgctcc aaatccattg gccaggccaa gcagcgcggt
     5401 gaagacattg cgccgctgtt ggcggaagtg gaccagatgg gcagcgatct ggagcagtgc
     5461 aagcgtgacc tcgacagcat tcagactgaa ctcgataacc tgctgctgaa tatccccaac
     5521 ctgccgcacg agtcggtacc ggtcggtgca gacgaagagg gcaacgtcga agtatcgcgc
     5581 tggggcgcgc cgcgcagctt cgatttcccg attcaggatc acgttgccct cggtgagcag
     5641 cacggttggc tggatttcga gaccgccgcc aaactctccg gtgcgcgctt tgccttgctg
     5701 cgcggcccca tcgcccgcat gcaccgcgct ctggcgcaat ttatgattaa cctgcacacc
     5761 ggcgagcatg gctacgaaga agcctacacg ccgtatttgg tgcaagcgcc tgcgttgcaa
     5821 ggcaccggcc agctgccgaa gttcgaggaa gacctgttca agatcagccg cgaaggcgaa
     5881 gccgatcttt acctgatccc gactgctgaa gtgtcgctga ccaatatcgt tgctggcgag
     5941 atccttgatc ccaagcagct gccactcaag ctggttgcgc acaccccgtg cttccgtagc
     6001 gaagccggcg catcgggtcg tgatacccgc gggatgattc gtcagcacca gttcgacaag
     6061 gttgagatgg tgcagatcgt cgagccgagc aaa
//
LOCUS       DASRUM010000096        19163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2058194, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000096 DASRUM010000000
VERSION     DASRUM010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 19163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19163
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2058194"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..961
                     /locus_tag="VFF22_06530"
     CDS             134..961
                     /locus_tag="VFF22_06530"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX16504.1"
                     /translation="MLSPYMSPIERIWHYSLRSLCGLVLLFLILPVLVIIPLSFNSGS
                     FLVYPLQGFSLRWYEDFFTSAEWMRSLKNSLIIAPAATLLAMVFGTLAAIGLTRGEFR
                     GKALVMSLLISPMVVPVVIVGVASYLFFAPLGLGNSYLSLIVVHAVLGVPFVIITVSA
                     TLQGFNYNLVRAAASLGASPLTAFRRVTLPLIAPGVISGALFAFATSFDEVVVTLFLA
                     GPEQTTLPRQMFSGIRENLSPTIAAAATLLIGFSILLLLTLEWLRGRSEKLRTQQPS"
     gene            991..2442
                     /gene="gabD"
                     /locus_tag="VFF22_06535"
     CDS             991..2442
                     /gene="gabD"
                     /locus_tag="VFF22_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZX16505.1"
                     /translation="MQLNDPSLLRQQAYVNGQWCEADSGARTEIRNPANGELVGSVPN
                     MGRAETRRAIEAAQTAQPAWRALTAKERANRLRQWFNLILANQEDLARIMTAEQGKPL
                     AEARGEIAYAASFIEWFAEEAKRAYGDVIPAHAGDKRILVQKEPVGVTAAITPWNFPC
                     AMITRKAGPALAVGCAMVLKPAPQTPFSALALAALAERAGIPAGLLSVIPADVDASRE
                     VGGELCDNPIVRKLSFTGSTSVGIKLMQQCAPTLKKLSLELGGNAPFIVFDDADLDAA
                     VEGAIISKYRNAGQTCVCANRLYVQDGVHDAFAEKLQAAVAKLRVGNGMEEGITTGPL
                     INAEAVAKVQRHLHDALEKGATLLAGGNSLGGNFFEPTIVTGVTTEMAVAREETFGPL
                     SPLFRFSDEAEVIRQANDTEFGLAAYFYARDLGRVFRVAEALEYGMVGINTGVISTEV
                     APFGGMKSSGLGREGSKYGLDEYLEIKYLCMGM"
     gene            2518..3798
                     /gene="gabT"
                     /locus_tag="VFF22_06540"
     CDS             2518..3798
                     /gene="gabT"
                     /locus_tag="VFF22_06540"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HZX16506.1"
                     /translation="MSKTNESLMQRRTAAVPRGVGQIHPIFAESAKNATVTDVEGREF
                     IDFAGGIAVLNTGHLHPKVVAAVQEQLTKLTHTCFQVLAYEPYVELCEKVAARVPGDF
                     AKKTLLVTTGSEAVENAVKIARAATGRAGVIAFTGGYHGRTMMTLGLTGKVNPYSAGM
                     GLMPGGIFRAQYPCALHGVSPDDAIASIERIFKNDAEPRDIAAIIIEPVQGEGGFYVA
                     PKDFMLRLRALCDQHGILLIADEVQTGAGRTGTFFAMEQMGVAADLTTFAKSIAGGFP
                     VAGVCGKAEYMDAIAPGGLGGTYAGSPIACAAALAVMEVFEEEKLLDRCKAVGERLVT
                     GLKAIQAKHKSIGEVRALGAMIAMELCEDGDLHKPNTALTGQIVAKARDKGLILLSCG
                     TYGNVLRVLVPLTAEDDLLDKGLAIIAECFDELA"
     gene            3927..4601
                     /gene="rpe"
                     /locus_tag="VFF22_06545"
     CDS             3927..4601
                     /gene="rpe"
                     /locus_tag="VFF22_06545"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZX16507.1"
                     /translation="MQPFAIAPSILSADFARLGEEVDKVLAAGADIVHFDVMDNHYVP
                     NLTIGPMVCAALRKYGITAPIDAHLMVKPVDRIIGDFIDAGASYITFHPEASEHIDRS
                     LQLIRDGGCKAGLVFNPATPLEVLKHVMDKVDMILLMSVNPGFGGQKFIPHTLEKLKE
                     ARALIDASGREIRLEIDGGVNVQNIREIAAAGADTFVAGSAIFNQPDYKAVIDAMRAE
                     LALARG"
     gene            4598..5317
                     /locus_tag="VFF22_06550"
     CDS             4598..5317
                     /locus_tag="VFF22_06550"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HZX16508.1"
                     /translation="MSLLRELFQGELPRLVMFDLDGTLVDSVPDLAAAVDNTLRELGR
                     PVAGVEQVRNWVGNGARVLVRRALACGLEHAHVDNELAEQALELFMQFYAANHALTQV
                     YPGVQSTLDWLREQQVELAIVTNKPERFVAPLLDEKGLGGYFRWIVGGDTLPQQKPDP
                     AALLHVLQLARVDAAQALFVGDSRNDVLAAKAAGVPCVALSYGYNHGRPIAEENPAHV
                     LDCLSGLLRPAEPPILPGFAG"
     gene            5531..7024
                     /gene="trpE"
                     /locus_tag="VFF22_06555"
     CDS             5531..7024
                     /gene="trpE"
                     /locus_tag="VFF22_06555"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003194700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HZX16509.1"
                     /translation="MTREEFLRLAAAGYNRIPVACETLVDFDTPLSIYLKLADEANTY
                     LLESVQGGEKWGRYSIIGLPARTVLRAYGHEVLISVDGVEVERHQCEDPLAFVEQFQA
                     RYKVPTLPGLPRFNGGLVGYFGYDSVRYVEPKLAKGVNPDALGTPDILLMVSDAVVVF
                     DNLAGKMHAIVLADPAQADALEQGQARLQEILHKLRQPITPRLGVDLAAPAGAEPDFI
                     SSFKRDDYESAVKAIKEYILAGDCMQVVISQRMSIPFKAAPIDLYRALRCINPTPYMY
                     FFNFGDFHVVGSSPEVLVRLEDGLVTVRPIAGTRPRGATEEADLALEEDLLSDAKEVA
                     EHLMLIDLGRNDVGRVASTGSVKLTEKMVIERYSNVMHIVSNVTGQLKEGLTAMDALR
                     AILPAGTLSGAPKIRAMEIIDELEPVKRGVYGGAVGYLAWNGNMDTAIAIRTAVIKDG
                     ELHVQAGAGIVADSVPALEWEETLNKRRAMFRAVALAEQTVPDQSKR"
     gene            7036..7632
                     /locus_tag="VFF22_06560"
     CDS             7036..7632
                     /locus_tag="VFF22_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HZX16510.1"
                     /translation="MLLMIDNYDSFTYNVVQYLGELGADVHVIRNDELSIAEIEALQP
                     ERIVVSPGPCTPTEAGVSIEAILHFAGKLPILGVCLGHQSIGQAFGGDVVRARQVMHG
                     KTSPVFHKDLGMFAGLNNPLTVTRYHSLVVKRETLPDCLEVTAWTQFENGEVDEIMGL
                     RHKTLHIEGVQFHPESILTEQGHELFANFLKQQGGVRP"
     gene            7629..8675
                     /gene="trpD"
                     /locus_tag="VFF22_06565"
     CDS             7629..8675
                     /gene="trpD"
                     /locus_tag="VFF22_06565"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HZX16511.1"
                     /translation="MNIKEALNRVVSQLDLNTAEMQDVMREIMTGQCSDAQIGAFLMG
                     LRMKSETIDEIVGAAQIMRELAAPVQIDAAHLVDTCGTGGDGMNIFNVSTAAAFVVAA
                     AGGQVAKHGNRAVSGKSGSADLLEAAGVYLNLAPEQVARCVESVGVGFMFAPAHHGAM
                     KYAIGPRRELGLRTIFNMLGPMTNPAGVKHQVLGVFSKALCRPLAEVLQRLGSEHVLV
                     VHAQDGLDEISLAAPTFVAELKDGVVSEYSIQPEDFGIKSQSLIGLTVEDAAESLTLI
                     RDALTKRKTEQGQKAADLIALNAGAAIYAADLAATLKEGVQRAHDALHTGLAWEKLQE
                     LVSFTAVYKQENAG"
     gene            8672..9508
                     /gene="trpC"
                     /locus_tag="VFF22_06570"
     CDS             8672..9508
                     /gene="trpC"
                     /locus_tag="VFF22_06570"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HZX16512.1"
                     /translation="MSLPTVLEKILARKAEEVAERRAIVSLAEVEQRARAADAPRGFA
                     NALLAQAKRKQPAVIAEIKKASPSKGVLREHFVPAEIAQSYAAGGATCLSVLTDVDFF
                     QGADSYLQEARAACSLPVIRKDFMIDPYQVVEARALGADCVLLIVSALDDGQMAELAA
                     TAKAFDLDVLVEVHDGDELERALKTLDTPLLGINNRNLHTFELNLETTLDLLPRIPRD
                     RLVVTESGILNRADVELMEISEVYAFLVGEAFMRADNPGAELERLFFPERKRVIASPD
                     VD"
     gene            complement(9569..10213)
                     /gene="crp"
                     /locus_tag="VFF22_06575"
     CDS             complement(9569..10213)
                     /gene="crp"
                     /locus_tag="VFF22_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HZX16513.1"
                     /translation="MVAITLTPKIKNLDKLLAHCHRRRYTAKSTIIYAGDRCETLFFI
                     VKGSVTILIEDDDGREMIIAYLNSGDFFGEMGLFEKDGTEKERSAWVRAKTECEVAEL
                     SYAKFRELTQQDPDILYALGSQMAERLRNTTRKVGDLAFLDVTGRVARTLLDLCKQPD
                     AMTHPDGMQIKITRQEIGRIVGCSREMVGRVLKALEEQGLVNVKGKTMVVFGTR"
     gene            10449..10874
                     /locus_tag="VFF22_06580"
     CDS             10449..10874
                     /locus_tag="VFF22_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZX16514.1"
                     /translation="MKARIQWAGEALFIGESGSGHAVVMDGPPESGGRNLGVRPMEML
                     LIGLGGCSNFDVVSILKKSRQAVESCEAFLEAERATEEPKVFTKIHLHFVVKGRGLKE
                     AQVKRAVELSAEKYCSASIMLGRGGVDITHDYEIIELGE"
     gene            complement(10942..11589)
                     /gene="coq7"
                     /locus_tag="VFF22_06585"
     CDS             complement(10942..11589)
                     /gene="coq7"
                     /locus_tag="VFF22_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HZX16515.1"
                     /translation="MASERHFSPVDRLLLQADAALRTLLPFSGQPTRPSPAIVQPEAE
                     LDEQQAQHVAGLMRINHTGEVCAQALYQGQALTAKLPQVRKAMEHAADEEIDHLAWCE
                     QRIRQLGSQPSILNPLFYGLSFGVGAAAGLISDKISLGFVAATEDQVVKHLDEHLQQI
                     PLEDQKSRAILEQMRVDEEQHACNALDAGGLRFPAPVKFGMSLLAKVMTGTTYRV"
     gene            complement(11682..12515)
                     /locus_tag="VFF22_06590"
     CDS             complement(11682..12515)
                     /locus_tag="VFF22_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HZX16516.1"
                     /translation="MATGLNRSLLGALTVLALLVINTAQAHTVGSVFSDALPTEKQGP
                     QLVVVPAGRYLLGDHSGRGNHNERPLTPIEIPQPFAIGRYEVSFSDWQHYADATATAM
                     PDNEGWGLSAQRPVIHVSWHQAVAYSQWLSKVTGQRYRLPTEAEWEYAARAGSTSYYW
                     WGEQLDSPETRPRAHCRGCATSRLIQSKTERVGQFAANAFGLHDTAGNVWEWTASRFA
                     SPFDGSEQRSAGLMDQSPRAVRGGAWNSGPSYLRSSQRDMKQPQHKDYALGFRVLREL
                     P"
     gene            complement(12500..12838)
                     /locus_tag="VFF22_06595"
     CDS             complement(12500..12838)
                     /locus_tag="VFF22_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HZX16517.1"
                     /translation="MDCLFCKIVAGAIPARKLYEDDQVIAFHDIGPQAPVHFLVIPKK
                     HISTLNDLSEADKPLAGHILFTAQRLAKEQGCEEGFRVVMNCNDLGGQTVHHIHMHVL
                     GQRQLAWPPG"
     gene            12893..14374
                     /locus_tag="VFF22_06600"
     CDS             12893..14374
                     /locus_tag="VFF22_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZX16518.1"
                     /translation="MKNDIHDLGLVLDSKTKLVLIESWDELRVLETLTSLAVRRSLGL
                     QLWSVTEGLQRVGFSIQEPVDSTTLEPETALRLIKADTQPTLYVMCDLHPFLEDNPKL
                     VRLLKEIAMNEQPSKPTLVLVSHACKLPPEVQRYASRFSLSLPSEEELLAIVRDEATA
                     WSARNRNSRVRTDNRTLQQVVKNLRGLSHAEARALARNVICDDGAITQEDLPELNKTK
                     FKLLDLDGVLSFEYDTARFAEVGGLSNLKRWLGDRQSIFLEGKGVDLPKGVLLVGVQG
                     GGKSLAAKAIAGLWGLPLLRLDFACLYNKFFGETERNLREALKLAEQMAPCVLWMDEI
                     EKGLASGDHDGGVSQRVLGTLLTWMAERKAPVFMVATANVISRLPPELVRKGRFDELF
                     FVDLPDAAVRAEIFRIHLARRELDVTGFDLAQLAAACDGFSGAEIEQAVVSALYASQA
                     QQQTVNQALLLQSIQGTAPLSVVMAEDLAALRAWADGRTVNAG"
     gene            complement(14384..15175)
                     /locus_tag="VFF22_06605"
     CDS             complement(14384..15175)
                     /locus_tag="VFF22_06605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX16519.1"
                     /translation="MTRYALITGASSGIGLALAEALARRGRNLILVARQRDALESIAC
                     ELTQRFSVEVLFRVCDLSQPLQVSGLLHELEQGERTIDLLVNNAGIGTSGTYVAQDWA
                     REQQLIELNILALARLCHAIGTIMAGQGSGQILNVASVAGFQPGPGMSNYYASKAYVL
                     HFSEGLREELKEYGVKVSVLCPGPTRSAFFRTARMNSEALQGSKLMMSAEEVALITVK
                     ALEKNRAIIIPGWRNRLIALSPRLGPRWLVRRISARLNRQFGTPI"
     gene            complement(15233..16177)
                     /locus_tag="VFF22_06610"
     CDS             complement(15233..16177)
                     /locus_tag="VFF22_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HZX16520.1"
                     /translation="MTQARFKIVFNGELMPDVSLETAKENLARLFKSDLTRINSLFSG
                     GNVDIKRDLSENEADQYLKALQSAGAKVRKEQDLAASLSLVETDDHRTESSEAESNVP
                     MTCPKCGHQQSKAVECSACGIVIEKFIARQAMLAENPPEVVSAATTPYATPKAAVAET
                     LPEFGELKAFTTDGRIGRLRYLAWSMVLMLACLPLFGIAGGFFAASEILGGLLMVVVG
                     IAVAVVGIMFGVQRLHDIGWSGWLLLVTLVPIVGGVFSLLMFIIPGSTAANRFGPPPP
                     PNSRAVKILALLWVAIIVIGIVAAIALPAYMGYSDAGL"
     gene            complement(16286..17548)
                     /locus_tag="VFF22_06615"
     CDS             complement(16286..17548)
                     /locus_tag="VFF22_06615"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZX16521.1"
                     /translation="MKRRNLLGLGALGLLGGWALRPSDLGQPHAPYFAALNQLLRRAG
                     AGLPLLVVDLERLDQNAELLASQLGKQLPLRLVAKSLASTGLLDYLAKKLGTQRFMVF
                     HQPHINQLAVSFPQSDLLLGKPLPSAAALSFYQQLPQGSGFVPAEQLTWLIDSQPRLQ
                     EYAALANALNQPLQIAFEIDIGLARGGFATPAELGAALTWLRNTPNRLRVRGLMGYDA
                     HLAHTPFWTEQASAFVQSAARYRAFISSSAAFSELWPQQPLLNGAGSLTYALHAKLDT
                     PLNEVAVGSALLKPSDFDSDLLAAQQAALWIASPVLKALPGQLPFLDSSHHLLQRWNR
                     NRQQAYYLYGGQWPAAPVSPAGLGYDSLYGRSANQERLIGSAGTGLQVDDWVFLRPQR
                     SEGLFALFNQLYLLRNGRLVGSWSANSA"
     gene            complement(17545..18507)
                     /locus_tag="VFF22_06620"
     CDS             complement(17545..18507)
                     /locus_tag="VFF22_06620"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HZX16522.1"
                     /translation="MSLPALLEQRLRLPVVAAPMFLVSNPQLVLACCNNGIVGSFPAL
                     NQRDSSGFKNWLQEIEAGLDSTAAPYAVNLIVHGSNPRLQADLAICVEQRVPIVITSL
                     GAVKEVVDAVHSYGGLVFHDVTTRRHAEKAAEAGVDGLIAVAAGAGGHAGTWSPFALI
                     AEIRQFFNKTLLLAGCLNQGHEVLAAQLLGADLAYLGTRLIATQENAASAAYKQMILD
                     AKAADIVHTPAVSGVPASFMRQSLELAGYDLKQLQNKGEVNYGEKLKPMDEEAKAWKT
                     VWSAGQGVGNIRDLPSVEQLIARLDSEYRAALKHSLQLQQRWAR"
     gene            complement(18529..18957)
                     /gene="hemJ"
                     /locus_tag="VFF22_06625"
     CDS             complement(18529..18957)
                     /gene="hemJ"
                     /locus_tag="VFF22_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019650354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HZX16523.1"
                     /translation="MLYLWLKAFHIIAMVCWFAGLFYLPRLFVYHAMSTDEVSRERFC
                     IMERKLYRGIMWPSLILTVGLGLWLISLNPSYYFSQGWMHAKLSLVALLVVYQFMCGA
                     QLKAFARGENQRGHVFYRWFNEAPVLALLGIVILVVVRPF"
BASE COUNT         3508 a         5737 c         6175 g         3743 t
ORIGIN      
        1 gtggtgtaca gctggctggt cggtgccagc aagttgcgcc tggcctaaga acagcctgcg
       61 ccgcaggtct atgaatatcc ctcgcccgtt gagtgggcga ggggagctga atgctcacct
      121 ggagtaaacc gtcatgctca gtccgtatat gtcccccatc gagcgcatct ggcactacag
      181 cctgcgcagc ctctgcgggc tggtgctgct gtttctgatc ctgcctgtgt tggtgatcat
      241 cccgctgtcg ttcaactccg gcagttttct ggtgtacccg ctgcaggggt tttctctgcg
      301 ctggtatgag gacttcttca cctcggctga gtggatgcgc tcgctgaaaa acagcttgat
      361 cattgccccg gccgccacgc tgctggcgat ggtgttcggc acgctggccg cgattggcct
      421 gacccgtggc gagtttcgtg gcaaggccct ggtgatgagt ctgctgattt cgcccatggt
      481 ggtaccggtg gtgatcgtcg gggtggccag ttacctgttt ttcgcgccgt tgggtttggg
      541 caacagctac ctgtcgctaa ttgtggtgca tgcggtgctc ggcgtgcctt tcgtcatcat
      601 caccgtgtcg gccaccctgc aaggctttaa ctacaacctg gtgcgcgcgg ccgccagcct
      661 cggtgcttcg ccgctgaccg cgtttcgccg ggtgaccctg ccgctgatcg cgccgggggt
      721 gatcagtggg gcgctgtttg cctttgccac ctcctttgat gaagtggtgg tgaccctgtt
      781 cctcgccggc cccgagcaga ccaccctgcc gcggcagatg ttcagtggca tccgcgaaaa
      841 cctgtcgccg actatcgccg ctgcggcaac cctgctgatc ggtttctcca tcctcctgct
      901 gttgaccctg gaatggctgc gcggccgtag cgagaagctg cgcacccagc agccgtcctg
      961 aaccacccag aacaagagag ccccatgagc ctgcaattga atgatcccag cctgctgcgc
     1021 cagcaggcct atgtgaatgg ccagtggtgc gaggccgaca gtggcgcgcg caccgagatt
     1081 cgcaatccgg ccaacggcga gctggtcggc agcgtgccca atatgggccg cgcggaaacc
     1141 cgccgcgcta tcgaggctgc acaaaccgcg caaccggctt ggcgtgccct gacggccaag
     1201 gagcgggcca atcgcctgcg ccagtggttc aacctgatcc tggccaacca ggaagacctg
     1261 gcgcggatta tgaccgccga acaaggtaaa cccttggctg aggcccgtgg cgagatcgcc
     1321 tacgccgcct cctttatcga gtggttcgcc gaagaagcca agcgcgccta tggcgacgtg
     1381 attccggccc acgctggcga taagcgcatc ctcgtgcaaa aagagccagt gggcgttacc
     1441 gccgccatta cgccgtggaa cttcccctgc gccatgatca cgcgcaaagc cggcccggcg
     1501 ctggccgtgg gctgcgccat ggtgctcaag cctgctccgc aaacgccgtt ctcagcgctg
     1561 gcgctggcgg cactggccga gcgcgctggt ataccggccg ggctgctgag cgtgattccg
     1621 gcggacgtgg acgccagccg tgaagtgggc ggcgagctgt gtgacaaccc catcgtgcgc
     1681 aagctgagct ttaccggctc caccagcgtc ggcatcaaac tgatgcagca gtgcgcgccg
     1741 accttgaaga agctctcgct ggaactgggc ggcaacgcac cgtttatcgt cttcgacgac
     1801 gccgacctgg atgccgccgt ggaaggggca atcatctcca agtaccgcaa cgccgggcag
     1861 acctgcgtgt gcgccaatcg cctgtatgtg caggacggcg tgcacgacgc ctttgccgaa
     1921 aaattgcagg cggcggtggc caaactccgg gtcggcaacg gcatggagga gggcatcacc
     1981 actggcccgt tgatcaatgc cgaggctgtg gccaaggtcc agcgccactt gcatgacgcc
     2041 ctggaaaagg gcgcgactct gctggcgggc ggtaacagtt tgggcggtaa tttctttgaa
     2101 ccgaccatcg ttaccggcgt gacgaccgag atggccgtgg cccgtgaaga aaccttcggt
     2161 ccgctgtccc cgctatttcg ctttagcgat gaggccgagg tgattcgcca agccaacgac
     2221 accgagttcg gcctggccgc ctacttctat gcccgcgacc tgggccgtgt attccgtgtg
     2281 gccgaagcgc tggagtacgg catggtcggc atcaacaccg gggtgatctc aaccgaggtc
     2341 gcgccgtttg gcggcatgaa gtcctccggt ctgggccgag aaggctcgaa atacggtttg
     2401 gatgagtatc tggaaatcaa atacctgtgc atgggcatgt agggtgcgcc gtgcgcacct
     2461 tgtctatttt cggtgcgcac ggcgcaccct accgttcacg tttcgtctga ggacactatg
     2521 agcaagacca acgaatcctt gatgcaacgc cgcaccgctg ccgttccacg cggcgtgggc
     2581 cagatccacc cgatcttcgc cgagtcagcg aagaacgcca ccgtgaccga cgttgaaggc
     2641 cgcgagttta tcgactttgc tggcggcatc gctgtgctca atactggcca cctgcacccg
     2701 aaagtggtgg cggcggtgca agagcagctg accaagctga cccacacctg cttccaggtg
     2761 ctggcctacg agccctacgt cgagctgtgc gagaaggtcg ccgcgcgcgt accgggcgat
     2821 ttcgccaaga agaccctgct ggtcaccacc ggctccgaag cggtggaaaa cgccgtgaag
     2881 attgcccgtg ctgccactgg ccgcgccggt gtgatcgcct tcaccggcgg ctaccacggt
     2941 cgcaccatga tgaccctggg tctgaccggc aaggtcaacc cctactcggc tggcatgggc
     3001 ctgatgcccg gcggcatctt ccgcgcccag tacccatgcg cgctgcatgg cgtgagcccc
     3061 gatgacgcga tcgccagtat cgagcgcatc ttcaagaacg acgccgagcc gcgcgatatc
     3121 gccgcgatca tcatcgagcc agtgcagggc gagggcggtt tctacgtcgc gccgaaagac
     3181 ttcatgctgc gcctgcgcgc cctctgtgat cagcacggca tcctgctgat cgctgacgaa
     3241 gtgcagaccg gcgccggccg taccggcacc ttcttcgcca tggaacagat gggcgtcgcc
     3301 gccgacctga ccaccttcgc caaatccatc gccggcggtt tcccggtggc cggcgtatgc
     3361 ggcaaggcgg aatacatgga cgccatcgcc ccaggcgggt tgggcggcac ctacgccggc
     3421 agcccgattg cctgcgccgc ggccctggcg gtgatggagg tgttcgagga agaaaagctg
     3481 ctggatcgct gcaaggctgt cggcgagcgt ctggtcaccg gcctcaaggc catccaggcc
     3541 aagcacaaga gcatcggtga agtgcgcgcc ctcggcgcca tgatcgccat ggaactgtgc
     3601 gaagacggcg atctgcacaa acccaacacc gccctgaccg ggcagatcgt cgccaaggcc
     3661 cgcgacaagg gcctgatcct gctgtcctgc ggcacctacg gcaacgtgtt acgcgtactg
     3721 gtaccgctga ccgccgaaga tgatctactc gacaagggcc tggcgattat cgccgagtgc
     3781 tttgacgagc tggcctgact ccgggattgt gccgcgcgcc tcggtgcgcg cggcgcaccc
     3841 tacggtcgtc gaccatcgtc ccagcgacgc ctgagaatcg gctcgggtac aatgcgcgcc
     3901 tttcgtgaat gccaccgagg tgcgttatgc aacccttcgc cattgctccg tcgattcttt
     3961 ccgctgactt cgctcgcctg ggcgaggaag tggacaaggt gctcgccgcc ggcgctgaca
     4021 tcgtgcactt cgatgtaatg gacaatcact atgtgccgaa cctgaccatc ggcccgatgg
     4081 tctgcgccgc gctgcgcaag tacggcatca ccgcgccgat tgacgcgcat ctgatggtta
     4141 agccagtgga ccgcattatc ggtgacttta tcgacgctgg cgccagctat atcaccttcc
     4201 atccggaagc cagtgagcat atcgaccgct ccctgcagtt gattcgcgat ggcggttgca
     4261 aggccggcct ggtgttcaat ccggcaaccc cgctggaagt gctcaagcac gtgatggaca
     4321 aggtcgacat gattctgctg atgagcgtca acccaggctt cggcgggcag aagtttattc
     4381 cccatactct ggagaagctc aaggaagcgc gcgcgctgat cgacgcatct gggcgtgaaa
     4441 tccgtttgga aatcgatggt ggagtgaatg tgcagaacat tcgcgaaatc gccgcagcgg
     4501 gtgccgatac ctttgtcgcg ggctcggcga tcttcaatca gcccgactac aaggctgtga
     4561 tcgacgccat gcgcgctgaa ttggccctgg cgcgcggatg agtctgctgc gtgagctgtt
     4621 ccaaggtgag ttgccgcgcc tggtgatgtt tgacctggac ggcaccctgg tggactcagt
     4681 gccggatctg gccgccgctg tcgacaatac cctgcgcgag ctgggtcgtc cggtagccgg
     4741 tgtggagcag gtgcgcaatt gggtcggcaa tggcgcgcgg gtgctggtgc gccgtgcgct
     4801 ggcgtgcggg ctggaacatg cgcatgtcga taatgagctg gccgagcagg cgctggagct
     4861 gtttatgcag ttttacgctg caaaccacgc cctgacccag gtctaccccg gcgtgcaatc
     4921 caccctggat tggttacgtg agcagcaggt cgagctggcg attgtcacca acaagcccga
     4981 gcgctttgtt gcgcccttat tggatgaaaa gggcctgggc ggctatttcc gctggattgt
     5041 cggcggcgac actctgcctc agcagaagcc cgaccccgca gcgctgctgc atgtgctgca
     5101 actggcccga gtggacgctg cgcaggcgct gtttgtcggt gactcgcgca atgacgtgct
     5161 ggcggccaag gcagccggcg tgccctgtgt ggcgctgagt tacggttata accacggtcg
     5221 gccgattgcc gaggagaatc ctgcgcatgt gctggattgt ctgagcggct tgctgcgccc
     5281 ggctgagccc ccgattttgc cggggtttgc gggttgattc ggctccggcc ctgcgctagt
     5341 gtggccgaac ttcaatcccg cctgaaataa gagagcttcg tggtggttac ctgcacgcgc
     5401 tggtcggcaa gcaccgacat gaaaatcatc aaggccctgg cccgttggcg ctggcgcccc
     5461 tgacctgatc ttggccgcct aggcggcgcg tttgcactcg accgaattac ctcaagccac
     5521 gaggctgatc atgacccgcg aagaattcct gcgtttagcc gctgccggct ataaccgcat
     5581 tcctgttgcc tgcgaaaccc tggtggattt cgacaccccg ctgtcgatct atctgaagct
     5641 ggccgatgag gccaatacct acctgctcga gtccgtccaa ggcggcgaga agtggggacg
     5701 ttactccatc atcggtctgc cggcccgcac tgtgctgcgg gcttatggcc atgaggtgct
     5761 gatcagcgtc gacggcgtcg aggtcgagcg ccatcaatgc gaagacccgt tggcctttgt
     5821 cgagcagttt caggcacgct acaaggtacc gactctgccg ggcctgccgc gcttcaacgg
     5881 cggcctggtg ggttacttcg gctatgacag cgtgcgttat gtcgagccca agctggccaa
     5941 gggcgtaaac cctgacgcgc tgggtacgcc ggatatcctg ctgatggtct ccgatgcggt
     6001 ggttgtattc gacaaccttg ccggcaagat gcacgccatc gtcctcgcag atccggccca
     6061 ggccgatgcg ctggagcagg gccaggcgcg tctgcaggag attctgcaca aactgcgtca
     6121 gccaattacc ccgcgcctgg gtgtggatct ggctgcgccg gcgggtgccg agccggattt
     6181 catttccagc tttaaacgcg atgactacga aagtgcggtc aaggcgatca aggagtacat
     6241 cctcgccggc gactgtatgc aggtagtgat ctcccagcgc atgtcgatcc cgttcaaggc
     6301 cgcaccgatt gatctgtacc gcgcgctgcg ttgcatcaac ccaacgccct atatgtactt
     6361 cttcaacttc ggcgacttcc atgtagtcgg cagctcgccg gaggtgctgg tgcgcctgga
     6421 agatggtctg gtcacggtgc ggccgattgc cggcacccgc ccgcgcggcg ccaccgaaga
     6481 ggccgatctg gccctggaag aggacctgtt gtcggacgcc aaggaagtcg ccgagcacct
     6541 gatgctgatc gacctaggcc gcaacgatgt cggtcgggtt gccagcaccg gcagcgtcaa
     6601 actcaccgag aagatggtga tcgagcgcta ttccaacgtt atgcatatcg tctccaacgt
     6661 caccggccag ctcaaggaag gcctgacggc gatggacgcg ctgcgcgcca tcctgccggc
     6721 cggcaccctg tccggcgcgc cgaaaattcg cgccatggaa atcatcgacg agctggaacc
     6781 ggtcaagcgc ggcgtttacg gaggtgccgt gggttatctg gcctggaacg gcaatatgga
     6841 cactgccatc gccatacgta ccgcggtgat caaggacggc gagctgcatg tgcaggccgg
     6901 tgctggcatc gttgccgact cggtgccggc cctggagtgg gaggaaacgc tgaacaagcg
     6961 tcgcgccatg ttccgtgctg tggcgctcgc cgagcaaacc gtccctgacc agtccaagcg
     7021 ctgaggagtc ccgccatgct tttaatgatc gataactacg actcctttac ctacaacgtg
     7081 gtgcagtacc tgggcgagct gggtgctgat gtgcatgtga ttcgcaatga cgaactgtcg
     7141 attgccgaga tcgaggcgct gcagcccgaa cgtatcgtgg tgtcgccagg cccgtgcacg
     7201 ccgaccgaag ccggggtgtc tatcgaggcg atcctgcatt tcgccggcaa gctgcctatc
     7261 ctcggtgtgt gcctcggcca ccagagcatt ggtcaggcct ttggcggcga tgtagtgcgg
     7321 gcgcgccagg tgatgcacgg caagaccagc ccggtgttcc acaaggacct gggtatgttc
     7381 gccggcctga ataatccgct gaccgtgacc cgctatcatt ccttggtggt caagcgcgaa
     7441 accctgccgg actgcctgga agtcactgcc tggacccagt ttgaaaacgg cgaagtggac
     7501 gaaattatgg gcctgcgcca caagaccctg catatcgagg gcgtgcagtt ccaccccgag
     7561 tcgatcctga ccgaacaggg tcatgaactc tttgccaact tcctcaagca acaaggaggc
     7621 gtgcgcccat gaatatcaag gaagccctta accgggtggt cagccagctc gacctgaaca
     7681 ccgccgagat gcaggacgtg atgcgcgaga tcatgaccgg tcagtgcagc gacgcacaga
     7741 tcggtgcctt tctgatgggc ctgcgcatga agagcgagac catcgacgag atcgtcgggg
     7801 ctgcgcagat catgcgtgaa ctggccgcgc cggtgcagat cgacgccgct cacctggtcg
     7861 acacctgcgg caccggtggc gacggcatga atatcttcaa cgtctccact gccgcggcct
     7921 tcgtggtcgc cgccgccggt ggccaggtgg ccaagcacgg caatcgcgcg gtgtcgggca
     7981 agagtggcag tgccgatctg ctggaagccg ccggggtcta cctcaatctg gctcccgagc
     8041 aggtggcgcg ctgtgtcgaa tccgtcggcg tcggttttat gttcgccccg gcccaccatg
     8101 gcgcgatgaa atacgccatc ggcccgcgcc gcgaactggg cctgcgcacc atcttcaata
     8161 tgcttgggcc catgaccaac ccggccgggg tcaaacatca ggtgctgggg gtattcagca
     8221 aggcgctgtg ccggcccctg gccgaggtgc tgcagcgcct gggcagcgag catgtgctgg
     8281 tggtgcacgc tcaggacggc ctggatgaaa tcagcctggc cgcgccgacc ttcgtcgccg
     8341 agctgaagga tggcgtggtc agcgagtaca gcattcagcc ggaggacttc ggcatcaaga
     8401 gccagagcct gatcggcctg accgtggagg atgccgccga gtccctgacg ctgatccgcg
     8461 atgccctgac caagcgcaag accgagcagg ggcagaaggc ggcggacctg atcgccctga
     8521 atgccggtgc ggcgatctac gccgccgatc tggctgctac cctcaaggag ggcgtacagc
     8581 gcgcccatga cgccctgcat accggcctgg cctgggaaaa actgcaggag ctggtgtcct
     8641 ttaccgccgt ttacaagcag gagaatgcag ggtgagcctt cccacagttc tggaaaagat
     8701 cctcgcccgc aaggccgagg aagtcgccga gcgccgcgcc atcgtcagcc tggccgaggt
     8761 cgaacagcgg gcgcgcgccg ccgatgcccc gcgcggcttt gccaacgccc tgctggcgca
     8821 ggccaagcgc aagcagccgg cggtgatcgc cgagatcaag aaggcctcgc cgagcaaggg
     8881 cgtgctgcgc gagcacttcg tgcccgccga gatcgcccag agttatgccg ccggcggggc
     8941 tacctgcctg tcggtgctga ccgatgtgga tttcttccag ggcgctgaca gctacttgca
     9001 ggaagcccgt gcggcctgct cgctgccagt gattcgcaag gactttatga tcgaccccta
     9061 ccaggtcgtc gaagcacgcg ccctgggtgc cgattgcgtg ctgctgatcg tctcggccct
     9121 ggatgacggg cagatggccg aattggcggc gaccgccaag gccttcgacc tcgatgtact
     9181 ggtggaggtg cacgatggcg acgaactgga gcgggcgcta aagaccctcg acacgccgct
     9241 gctgggcatc aacaaccgca acctgcacac cttcgagctg aatctggaaa ccaccctgga
     9301 tctgctgcca cggattcccc gcgatcgcct ggtggtgacc gagagcggca tcctcaatcg
     9361 cgccgatgtc gagctgatgg aaatcagcga ggtgtatgcc ttcctggtcg gcgaagcctt
     9421 tatgcgcgcc gataacccgg gggcggagct ggagcgactg ttttttcccg agcgcaagcg
     9481 ggtcatcgcc tcgcccgacg tggactgagc ctgcagacat aaaaaagccg acgcatgcgt
     9541 cggctttttt atgtctgcag catgcaactt agcgcgtgcc gaacaccacc atggtcttgc
     9601 ctttgacgtt gaccaggccc tgttcttcca gggccttgag cacgcggccg accatctccc
     9661 gggagcagcc gacgatgcgg ccgatttcct gacgggtgat cttgatctgc atgccatcag
     9721 ggtgggtcat ggcgtcgggt tgcttgcaca ggtcgagcag ggtgcgggca acgcggccgg
     9781 tgacatcgag gaaagccagg tcaccaactt tacgcgtggt gttgcgcagg cgctcggcca
     9841 tctggctgcc aagggcgtag aggatatccg gatcttgttg ggtcagttcg cggaacttgg
     9901 cgtagctcag ctccgccact tcgcactcgg tcttggcccg tacccaggcg ctgcgttcct
     9961 tctcggtgcc gtccttttcg aacaggccca tctcgccgaa gaagtccccc gagttgaggt
    10021 aggcgatgat catctcgcgg ccatcatcgt cttcgatcag gatggtgacc gaccccttga
    10081 cgataaagaa cagcgtctca caacgatcgc cagcatagat gatggtgctt ttcgccgtat
    10141 aacggcggcg gtgacagtgt gcgaggagtt tgtcgaggtt ctttattttt ggtgtaaggg
    10201 taattgcgac catgcccgag tcccgaaatt tataaggtaa gcctttgtcc gacaggcaga
    10261 tttatttttt tagttatcca ttcaagtgtg ccagtaaacc ggcgtcagct taacagagct
    10321 tgtctgcgca gggcttgttt tgcgacgtag ctaacatctg accgggaact cttgggttcc
    10381 ttgccggtat cgggttgcga aaagtgctgt gctaagctgc cttccttttt aacagcggag
    10441 cctgagtaat gaaagcgcgc attcagtggg ccggtgaagc cctgtttatc ggcgaatcgg
    10501 gcagtggcca tgccgtggtg atggatggcc cgccggagag cggtggccgc aatctgggtg
    10561 tgcgcccgat ggagatgctg ctgatcggcc tgggcggctg cagcaacttc gatgtggtca
    10621 gcatcctgaa aaaatcccgc caggcggtag agagttgcga agcctttctg gaagccgaac
    10681 gcgcgacgga agagcctaag gtatttacca agatccacct gcattttgtg gtcaagggcc
    10741 gcggtttgaa ggaggcgcag gtcaagcgtg cggttgaact gtcggccgag aaatactgct
    10801 cggcctcgat catgctgggc cgcggtggtg ttgatatcac ccatgactac gagattatcg
    10861 agctgggcga gtaaaacgct gcagatctcg tgacatgaaa aagggcacct cggtgccctt
    10921 ttctgtgcct ctgactcagc tttaaacccg gtaggtggtg ccggtcatta ccttggccag
    10981 caggctcatg ccgaacttca ccggtgccgg gaaacgcagg ccaccggcat ccagggcgtt
    11041 gcaggcatgc tgctcttcat cgacacgcat ctgttcgaga atcgcgcggg atttctggtc
    11101 ttccaacggg atctgctgca gatgctcatc gaggtgtttg accacctgat cttcggtggc
    11161 agcgacaaag cccaggctga tcttgtcgct gatcagtccg gcggccgcac cgacgccaaa
    11221 ggacaaaccg tagaacagcg ggttgaggat gctcggctga ctgcccagct ggcggatacg
    11281 ctgctcacac caggccagat ggtcgatttc ctcatcggcc gcatgctcca tggccttgcg
    11341 cacctgtggc agcttggcgg tcagggcctg gccctggtac agcgcctggg cgcagacctc
    11401 gccggtgtgg ttgatgcgca tcaggccagc gacatgctgg gcctgctgct cgtccagctc
    11461 ggcttccggt tgcacgatgg ccggcgatgg ccgggtcggt tgaccgctga aaggcagcag
    11521 ggtgcgcagc gcggcatcgg cctgcaggag caggcgatca acaggagaga agtgacgttc
    11581 gctggccata gggacctccg gcgatgatga tggcgctcag tttactccac gcgcgggaga
    11641 aggtctgttg acgtttcgtc gcagcctcgc cgcgctaggc ctcacggcaa ctcgcgcagc
    11701 acgcgaaaac ccagtgcgta gtccttgtgc tgcggctgct tcatatcgcg ctggctgctg
    11761 cgcaggtagc tcgggccact gttccaggcg ccgccacgaa ctgctcgcgg gctctgatcc
    11821 attaaaccgg cgctgcgttg ctcgctgcca tcgaacgggc tggcgaagcg tgaagcggtc
    11881 cactcccaga cattgccggc ggtgtcatgc aggccaaagg cattggcggc gaactggcct
    11941 acacgttcgg tcttgctctg gatcagccgt gaggtggcgc agccacggca atgcgcacgc
    12001 ggccgagttt ctgggctgtc gagctgctcg ccccaccagt aatagctggt gctgccggcg
    12061 cgggctgcgt attcccattc ggcttcggtg ggcaggcgat agcgctggcc ggtgaccttc
    12121 gacagccact ggctataggc tacagcctgg tgccatgaga catggattac cggccgctgc
    12181 gccgacaacc cccagccctc gttgtccggc atggctgtag cggtggcgtc ggcgtagtgc
    12241 tgccagtcgc tgaaactcac ctcgtaacgg ccaatcgcaa atggctgagg aatctcgatg
    12301 ggcgtcagtg ggcgttcgtt atggttgccg cggccactgt ggtcaccgag cagatagcgc
    12361 ccggcaggca ctaccaccag ctgtggccct tgcttttccg tgggcaaggc atcgctgaat
    12421 acgctgccaa ccgtatgcgc ctgcgcggta ttgatgacca gcagggcgag cacggtcagc
    12481 gcgccgagta atgaacgatt caacccggtg gccatgccag ctgccgctgt cccagcacat
    12541 gcatatggat gtggtgcacc gtctgaccgc ccaggtcatt gcagttcatt accacgcgga
    12601 aaccttcctc gcagccttgc tctttggcca ggcgttgcgc ggtaaagagg atatgcccag
    12661 ccagcggctt gtccgcctca ctcaagtcat tgagggtgct gatgtgtttt ttcgggataa
    12721 ccagaaagtg caccggtgct tgcgggccga tatcgtgaaa ggcaattacc tgatcatctt
    12781 cataaagctt gcgcgccggt attgcaccag cgacgatctt gcaaaataga cagtccatgg
    12841 acagtctccc gacctttaaa aagaggccga gtttagtgga caaggagcgt gggtgaaaaa
    12901 cgatattcat gatctgggct tggtactgga ttccaagacc aagctggtgc tgatcgaatc
    12961 ctgggatgag ctgcgggtgc tggaaaccct gaccagccta gcggtgcgcc gcagtcttgg
    13021 cttgcagctg tggtcagtga ccgagggttt gcagcgcgtg gggttttcca ttcaggagcc
    13081 tgtcgacagc acgacgcttg agccagaaac cgccctgcgt ctgatcaagg ctgacaccca
    13141 gccaacgctg tatgtgatgt gcgatctgca tccgtttctt gaggacaacc ccaagttggt
    13201 gcgcctgctc aaggaaatcg ccatgaacga gcagccgagc aagccgacgc tggtgctggt
    13261 ttcccacgcc tgcaagttgc cgccggaagt gcagcgctac gcctcgcgct tcagcctgtc
    13321 gctaccttcc gaggaagagc tgctggccat cgtgcgtgac gaggccactg cttggagtgc
    13381 acgcaaccgc aactcccggg tgcgcaccga taaccgcacc ttgcagcagg tggtgaagaa
    13441 cctgcgtggt ctcagccatg ccgaggcgcg tgcgctggcg cgcaatgtga tctgcgatga
    13501 cggcgcgatt acccaggaag acctgccgga gttgaacaag accaaattca agctgctcga
    13561 tctcgatggc gtgctgagtt tcgagtacga caccgcacgt ttcgccgagg tcggtgggct
    13621 gagcaacctc aaacgctggc tgggtgatcg gcagagcatc tttctcgaag gcaagggcgt
    13681 cgatttgccc aaaggcgtgc tgctggtcgg tgttcagggc ggcggcaaga gtctcgccgc
    13741 caaggccatc gccggactgt ggggcttgcc cctactgcgt ctggactttg cctgcctgta
    13801 caacaagttc ttcggtgaga ccgagcgcaa cctgcgtgaa gccttgaagc ttgccgagca
    13861 gatggcgcct tgtgtgctgt ggatggatga gatcgaaaag ggcctggcca gtggtgatca
    13921 cgatggtggc gtcagccagc gcgtactcgg caccctgctg acctggatgg ccgagcgcaa
    13981 ggcgccggta tttatggtgg ccaccgccaa cgtgatttca cggctaccgc cggagctggt
    14041 gcgtaagggg cgttttgatg agctgttttt cgtcgatctg ccggatgcgg cggtacgggc
    14101 cgagatcttc cggattcacc tggcgcgtcg cgagttggac gtgacaggct tcgacctggc
    14161 gcagctggcg gcggcttgtg acgggttttc cggcgcggag atcgagcagg cggtggtcag
    14221 cgccctgtat gcatcgcaag cccagcaaca gacggtcaat caggcattgc tgctgcaaag
    14281 catccagggt accgcgccac tgtcagtggt gatggccgaa gaccttgccg cgttgcgcgc
    14341 ctgggcggac gggcgcacgg tcaacgcggg ttagggctgt cgcttagatc ggggtgccga
    14401 actggcgatt aagccgtgcg ctgattcgcc ggaccagcca gcgcggtcct agccgagggc
    14461 tgagggcaat caggcggtta cgccagccgg ggatgatgat cgcgcggttt ttctccagcg
    14521 ccttgacggt gataagtgcc acttcttcgg cgctcatcat cagtttgctg ccctgtagcg
    14581 cctcactgtt catacgagcc gtgcggaaaa acgcgctgcg ggttggcccc ggacagagta
    14641 cggatacttt aacgccgtac tctttcagct cttcgcgcag gccttcggag aagtgcagca
    14701 cgtaggcctt gctcgcgtag tagttgctca tacccgggcc gggctgaaag ccggcaaccg
    14761 aggcgacgtt gagaatctgt ccgctgccct ggccggccat gatggtgccg atggcgtggc
    14821 agaggcgggc gagtgccagg atattcagtt cgatcagttg ctgttcgcga gcccagtcct
    14881 gggctacgta agtgcccgag gtgccgatgc cggcgttgtt cactagcaga tcaatagtgc
    14941 gttcgccttg ctccagttca tgcaacaggc cactgacctg cagtggctgg ctgaggtcgc
    15001 agacgcgaaa cagcacctcc acggaaaaac gttgagtcag ctcgcaggca atgctttcca
    15061 gcgcatcgcg ttggcgcgcc accagaatca gattgcggcc acgccgagcc agggcttccg
    15121 ccagggctag gccaataccg ctggaggctc cggtaataag cgcgtaacgg gtcatagggt
    15181 tctccgtgat ggggcctttc gaccccatgt accgcgcaag gttgcgagtg ggttacaggc
    15241 cggcgtcgga gtaacccata taggcaggca gggcgatggc cgcaacaata ccgatgacga
    15301 tgattgccac ccagagtagg gcgaggatct ttaccgcgcg gctattcggt ggcggcggcg
    15361 ggccaaagcg gttcgcggcg gtgctgccgg ggatgataaa catcagcagg gagaacaccc
    15421 cgccgacaat cggcaccagg gtgaccagca atagccagcc cgaccagccg atatcatgca
    15481 ggcgctgcac gccgaacatg atgccgacca ccgcgacggc gatacccacc acgaccatca
    15541 gcaggccgcc aagaatctcc gaagcggcga agaagccgcc ggcgatgccg aacaacggca
    15601 agcaggccag catcagcacc attgaccagg ccaggtaacg caggcggccg atgcggccgt
    15661 cggtggtaaa ggcttttagc tcgccgaatt ccggcaatgt ttctgccaca gccgcttttg
    15721 gcgtggcgta gggggtggtc gctgcgctga ctacttccgg cgggttttcc gcgagcatgg
    15781 cctggcgggc gataaatttc tcgataacga tgccgcaggc cgagcactcg acggctttgc
    15841 tttgctggtg gccgcacttg gggcaggtca ttggtacgtt gctttcagct tcgctgcttt
    15901 ccgtacggtg atcatcggtt tccaccaggc tcaggctggc ggcgaggtcc tgctccttgc
    15961 gcactttggc gccggcgctc tgcaaggcct tgaggtactg gtcggcctcg ttttcgctaa
    16021 ggtcgcgctt gatatcgaca ttgccgccgc taaacagcga gttgatccgc gtcaggtcgc
    16081 tcttgaacag gcgggcgagg ttttccttgg ctgtttccag gctgacgtca ggcatcaact
    16141 cgccgttgaa cacaatcttg aagcgggctt gggtcatgtc ggcgtccttg tcggtgccag
    16201 aatggagttg cgctacagac tagccggcct gcaggccagt aaacaagcgc gcctgcgcgc
    16261 aaatgtgcag ggttcttgag gtgcttcacg cgctgttggc ggaccagcta ccgaccaggc
    16321 ggccgttgcg taacaagtag agctggttga acagtgcgaa cagaccttcc gaacgttgcg
    16381 ggcggagaaa cacccagtca tcgacctgta agcctgtgcc ggctgagccg atcaggcgtt
    16441 cctggttagc gctgcggcca tacagcgagt catagccgag tccggccggg gacaccggcg
    16501 ctgccggcca ttgcccgcca tacaggtaat aagcctgctg gcgattgcgg ttccagcgtt
    16561 gcagcagatg gtgcgagctg tcgagaaacg gcagctgccc gggtagcgcc ttgagcactg
    16621 ggctggcgat ccacagggcg gcttgctggg cggccagcag atcgctgtcg aaatcactgg
    16681 gcttgagcag tgcggagccg accgcgactt cattcagcgg ggtatcgagc ttggcatgca
    16741 gggcataagt caggctgcca gcgccattga gcagtggctg ttgcggccac agttcgctga
    16801 atgcggcgga gctggagata aacgcccggt agcgcgcggc gctctggacg aaggcgctgg
    16861 cttgctcggt ccagaacggc gtatgggcga ggtgcgcgtc ataacccatt aagccgcgta
    16921 cgcgtagccg gttgggcgtg ttacgcagcc aggtgagtgc ggcgccaagt tctgcgggag
    16981 tcgcaaagcc gccgcgggcc aggccgatgt cgatttcaaa ggcaatctgc agcggctggt
    17041 tgagggcatt ggccagggca gcgtattcct gcagacgggg ctggctatca atcagccagg
    17101 tcagctgctc ggcggggaca aagccgctgc cttgtggcag ttgctggtag aaactcaggg
    17161 ccgctgcact gggcaagggt ttgccgagca gcaggtcgga ctgcggaaag ctcactgcca
    17221 gctgattgat atgtggctgg tggaacacca taaaacgttg cgtgccgagc ttcttcgcca
    17281 ggtagtcgag cagcccggtg gaggccagcg atttggccac caggcgcagt ggcagttgct
    17341 taccgagttg gctagccagc agctcggcgt tctggtcgag gcgctccagg tctaccacca
    17401 gcagcggcag tccggcgcct gcacgccgca gtaactggtt gagtgcggca aagtagggcg
    17461 cgtggggctg gccgagatcg ctcgggcgca gtgcccagcc gcctaataac cccagcgcac
    17521 caaggccgag caggttgcgt cgcttcatcg cgcccagcgt tgttgcagct gcaggctgtg
    17581 cttgagggcg gcccggtatt cgctgtcgag gcgggcgatc agttgttcga cgctgggcag
    17641 gtcgcggata ttgccgacgc cctggccggc tgaccacacg gttttccagg ccttggcttc
    17701 ttcatccatt ggcttgagct tctcgccgta gttgacctcg cctttgttct gcagctgctt
    17761 gaggtcgtag ccggccagct ccaggctctg gcgcataaag ctcgccggta cgccggagac
    17821 cgctggggtg tgaacgatgt cggcggcttt ggcgtcgaga atcatctgct tgtaagcggc
    17881 ggaagcagcg ttttcctggg tggcgatcag gcgggtgccg aggtaggcga ggtcggcgcc
    17941 aagcagttgc gcggccagca cttcgtggcc ctggttaagg cagccggcca gcagcagggt
    18001 cttgttgaag aactggcgga tttcggcgat cagcgcgaat gggctccagg tgccggcatg
    18061 tccgcctgca ccggcggcta ctgcgatcag gccgtcgacg ccggcttcgg cggctttctc
    18121 ggcatgccgg cgagtggtca cgtcgtggaa caccaggcca ccgtagctgt gcaccgcgtc
    18181 gaccacttcc ttcaccgcac cgaggctggt gatcacgata ggcacacgct gctcgacgca
    18241 gatcgccagg tccgcctgca ggcgcgggtt gctgccgtgc acgatcaggt tgaccgcata
    18301 gggcgcggcg gtgctgtcca ggcctgcttc gatctcttgt agccaattct tgaagccgct
    18361 gctgtcacgc tggttcagtg ctgggaagct gccgacgatg ccgttgttgc agcaggccag
    18421 caccagttgt gggttggaca ccagaaacat cggcgccgcg acgacgggca ggcgcaggcg
    18481 ttgttcgagc agggcgggaa gcgacatgcg gtaatcctct tgaggtattt agaaagggcg
    18541 aaccacgacc agaatgacaa tgccaagcaa ggccagcacg ggcgcttcat tgaaccagcg
    18601 gtagaacaca tggccgcgct gattctcgcc acgggcaaac gccttgagct gggcgccgca
    18661 cataaactgg tagaccacca gcagcgccac caggctcagc ttggcgtgca tccagccttg
    18721 gctgaagtag tagctcgggt tgaggctgat caaccacagg cccaggccga cggtaagaat
    18781 cagcgacggc cacatgatcc cgcgatacag cttgcgctcc atgatgcaga agcgctcgcg
    18841 gctgacctca tccgtgctca tggcgtggta gacgaacagg cgcggcaggt agaacaggcc
    18901 ggcgaaccag cagaccatgg cgatgatgtg aaaagccttg agccatagat agagcattgg
    18961 tcgaactcat tgcaggaaag tgcctcgata gtagtgggca ggaccgcctc cgtcatcact
    19021 gacggcgctc aagtgttggc ccctggccga agcggccgta tcatcggcag ctttccactg
    19081 ctggtattgc aaaggggcaa gtgatgatca aggtcggaat cgtcggcggc acggggtata
    19141 ccggtgtcga gctgttgcgt ttg
//
LOCUS       DASRUM010000097        19412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2225338, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000097 DASRUM010000000
VERSION     DASRUM010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 19412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19412
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2225338"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1551)
                     /locus_tag="VFF22_06630"
     CDS             complement(91..1551)
                     /locus_tag="VFF22_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZX16524.1"
                     /translation="MAIARDDLDQHGLIIGVSPERFRAVAMPLLFDHLNAQCEGTIAV
                     NRQARIVWINDKYAEKVGISDPRTALGKPIEEVLPASRLREVVESGQPSMLDLMAFGS
                     EHFVVTRIPLRDEDGTLVGALGFVLFDRARHLKPLMAKFNVLQNQLVATQHELAKARR
                     ARYTIAGFIGASTAASEVKRQARRAAQLDATVLLRGETGTGKELLAQGIHNLSPRANG
                     PFVAVNVAAIPETLVEAELFGTAPGAFTGADRKARIGKFEVANGGTLFLDEIGDLPLP
                     LQAKLLRVLQEQEVEPLGSNQVKPLNVRVIAATHIDLEAKVAAGQFRDDLYYRLNVLA
                     LRVPPLRERREDIPALLEHLLDDIANRSTQAPLEVSDDALALLSAQPWRGNVRELRNL
                     LERAQLDVDGRLDAQSVRALLVDPVTPAAHSIAPLPPSTVTQTLAEQLAHAERQALID
                     ALQASAGNRQQAAERLGISRAGLYAKLALHGLGKHD"
     gene            1672..1830
                     /locus_tag="VFF22_06635"
     CDS             1672..1830
                     /locus_tag="VFF22_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HZX16525.1"
                     /translation="MDLVRILIAILLPPLGVFLQVGFAGAFWLNILLTLLGYIPGIVH
                     AVYIIAKR"
     gene            2089..3174
                     /locus_tag="VFF22_06640"
     CDS             2089..3174
                     /locus_tag="VFF22_06640"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZX16526.1"
                     /translation="MFFRPRTKPGSDNSVAVDDLQDVGQRSLIRMIFASTGLTLGVFC
                     ALQFMAGNYLLASFELVATGALLWGAWCIVRVRNLLPWIYLYLLPTFSFLLYIIVIPN
                     ASKTAFVWVYSIPVLSYLLLGLKRGSMLALPFVIGACLLYLYTYPIQRDAESLIDLGN
                     AVFCGVMILVFVYLYEARRAAAYQQLQHMARTDALTGVASRGSFQQSLEQSIREAQRS
                     RAKLVLVVLDVDHFKMVNDQYGHDAGDQALRHICNSLSSRLRATDTLGRLGGEEFGLL
                     LRNTDGQGAEPLVETLREQLCSQPMRYGDEDIALSATFGLAEWPVDGSTAEQLYRCAD
                     QRLYRGKELGRNRLVGRSSTVDKCSAE"
     gene            3313..4674
                     /locus_tag="VFF22_06645"
     CDS             3313..4674
                     /locus_tag="VFF22_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZX16527.1"
                     /translation="MTVLSAFLKQVLKLWHSPDLALIGQRRERRMRMLASLVLLLVGS
                     LWGIFFSLRGEWGIVLMDLLMIFCGLGVFILTLRNRVRLANLLLFGVIILVIVGTTLL
                     MDPHTAAAPRATHLYLLPIAVAALMAFRDERLWLRYGVALFCLSLFTVLASSTWSPTS
                     AYHLPDDVRINGSWVQGVAAMLMLFALLHILQSDAAERSLLDRDLHAALREQQFVLHY
                     QPQVNSSGRVTGAEVLIRWQHPQRGLLPPAEFIGHAEKTGLIIPMGRWVLEQAFTQLH
                     AWKDDPLYRDISLAVNISQKQFRQASFVAEMLGLIETYGIDARRLELELTETLIVHDI
                     DDLMGKMAALVERGVRFSLDDFGTGFSSLSHLKRLPLNTLKIDRSFISDILTDANSQA
                     IVRTVIALAQSMGMTVIAEGVETQAQQQFLLSNGCVQFQGYLFSKPLPLGAFTAFVER
                     QNG"
     gene            5131..5310
                     /locus_tag="VFF22_06650"
     CDS             5131..5310
                     /locus_tag="VFF22_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019821495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HZX16528.1"
                     /translation="MTTKGNNNPGNFANDREKASEAGKKGGQSSGGNFANDREKASEA
                     GRKGGQQSHGGGRQS"
     gene            5366..5578
                     /locus_tag="VFF22_06655"
     CDS             5366..5578
                     /locus_tag="VFF22_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16529.1"
                     /translation="MRAAPEFNRGEIQHVSVVEVQPEAMLMTHLKTAAKSRLYIQGCY
                     EHKEGVEYAEGKTSAPHIPAVCEKAL"
     gene            5868..6419
                     /locus_tag="VFF22_06660"
     CDS             5868..6419
                     /locus_tag="VFF22_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017529078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HZX16530.1"
                     /translation="MPLHVINFTGPINASTCSQLIEKASLAVQRDASGLLLNLATMGG
                     ECSYGFTMYNFLMALPVPVHTHNLGTVESMGNIVFLAGQHRTACQHSKFLFHPFHWHL
                     QGAVDHSRMSEYAMSLDYDLQLYARIVAERTAHAREKLETEKYLTAAPRIVDPQQALL
                     SGLIHAIELPPISAEAVSSFIHT"
     gene            6600..7028
                     /locus_tag="VFF22_06665"
     CDS             6600..7028
                     /locus_tag="VFF22_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005765365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HZX16531.1"
                     /translation="MNATTHPTYRCRPGPLHATLLAGTVPLFLGGLLSDIAYFRSYQI
                     QWSNFASWLIAGALVFCGLALLFALVNLLRARPRSGRPLVYFLLLLATWVLGFINALE
                     HAKDAWATMPAGLVLSVIVTVLACVTTWIGLSNLRTGDAA"
     gene            7025..8320
                     /locus_tag="VFF22_06670"
     CDS             7025..8320
                     /locus_tag="VFF22_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017684647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HZX16532.1"
                     /translation="MNSIRTALTLTLALLLSACDGEQQGSAQHYGPAPTLPEPERGML
                     PSMKIAEPQPWGDQVPSVPENFSVTAIATDLKIPRQTLVLPNGDILVAEGRGGNAAKL
                     KPKDVIAGYIKAKGNTAVEGGDRLTLLRDADGDGRYELQQVFADNLNAPYGLAFYEGN
                     LYVANQDALVRFDYQDGQTEASGPPTKVIDLPSEINHHWTKALTISPDGRFLYVGIGS
                     NSNITERGMEAEVDRALVWQINAETGAYKPYATGLRNPTALAIQPETGLLWAVVNERD
                     ELGADLVPDYLTSVREGGFYGWPYSYWGKNIDPRVRPQKPEKVASAITPDYALGAHVA
                     ALGLDFSVPAMGENFSEGVFIGEHGSWNRKTPVGYKVIFVPFRDGKPSGPPVDFATGF
                     RTEAGKTRGRPVGVTVDPRGALIVADDLANTVWRISRLQ"
     gene            complement(8614..9297)
                     /locus_tag="VFF22_06675"
     CDS             complement(8614..9297)
                     /locus_tag="VFF22_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16533.1"
                     /translation="MAHLLRSFELFQPKLGAIVIAPESLLYSETDHFARQTLEEHYQF
                     QQIAELKRCTFHGARVQASVLQISPGAHHPSLQEMRPSSVVIHTTILRGSLPVHLNVS
                     DPTGVPFLHSTDIREVVTSGSTLELSKTSTVAKGRTKGWVILIPRVGVPDIQYVKAIK
                     LETTVQLSDCVIALEFQNKANAMKAERRIHSRWGDFIKIYRGTGARYITISRLNIWLP
                     SLALTTQNN"
     gene            complement(9668..10606)
                     /locus_tag="VFF22_06680"
     CDS             complement(9668..10606)
                     /locus_tag="VFF22_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16534.1"
                     /translation="MTTMPTPSLNTKRFKNITIADINADELIISAAESAENRDRLPLW
                     EQIHTSDPKRIILIKKIDKESILVTHNGTEKNVSLRDEDAINSIFVSKKILIDVTGLA
                     HNIWAPLLKSAYSQKIRTRVLYAEPESYTPHPSPASSAIFDLSVTFEGLAPLPGFVQL
                     AGPVDEDKCLFIAMLGFEGNRPEHLASQLDPTPKVIPVVGAPGFQLEYPAFTVGCNRM
                     FLDEYRAHSEVRYARASCPFEAFNSLSKIRRDYPDHYIYLALVGTKPHAIGSILFAIK
                     YAETTEIMFDYPVKKSGRTTGVGIVHIYDFEDFHAY"
     gene            complement(10603..12474)
                     /locus_tag="VFF22_06685"
     CDS             complement(10603..12474)
                     /locus_tag="VFF22_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16535.1"
                     /translation="MSELNPNFVLGQLFGDNRAEWPSANFKDLFVRPTYLGKLEVMRP
                     CFLVGGRGTGKTTALQSLRYDSMLERIEGEGQTFADQEYLGVLVRMNKNRVRAFSGSG
                     VGEERWSKLFAHYFNLIVCSELANLTSWLEVRLGLKLPEESLSEISNALGLDECGTLD
                     QLKSSIKKAISSLQLQVNNPLKDMEIMLSMAEAPLRTFVEVLKSAGLLGDRVILCCID
                     EYENLLDYQQAIINTYIKHAEPPLSYKMGVRKNGLRNRQTLDGQDLLRVPDDCLEIEI
                     ADEGFELFAKAVAELRLRYAQSVGSKVPNSLREFMQELSLSEEAIVLGADRIAENILS
                     ELDGSQHFEYFKQKSKGEIYFLKYWQESEGGSLKDLADDWYRNETEWKTRLGNHGYAS
                     LFWLSKGRKGARIRKYYCGERTLLSLAAGNIRYFLELIDTAISYQLNEEPSKQPLLTI
                     SPKSQTLAAREVGKRRLNQLEGLADHGVQLKRIVLAIGKVFFELARAPSGKTPEVTSF
                     VLSGSPAEASRIHELLQEGVAHLAFESDPRTKSTSSSELRDDEYRLHRIFSAFFEISH
                     RKKRRMTIEASTLISVLENRPSRAISTLLDDRPQTSEEELPEQLALFSAFYDAGDFK"
     gene            complement(12461..13357)
                     /locus_tag="VFF22_06690"
     CDS             complement(12461..13357)
                     /locus_tag="VFF22_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16536.1"
                     /translation="MRADTQIQEILAKCDILKRAGLWPSEPILRPRAWLMNFDEQDAY
                     VAAFLLDKFTFYNRVLTDSLLVASYNSIGDGMPKGTSAPLGNDLVAALGSAILTPVKG
                     ERPNPTDSGYLLCRKARQLLHLNDSFILDTDEALQHAYAGNTVVFIDDFVGSGDQFLY
                     TWQTADKAGRSFKKASDISGFTAIYVTLVTTDFGLDNINIRAPKVAVCATHVIEKKST
                     LQGVLDSNPSMNLPILRFLSKYSTRLTPAEPYIANNANYLMFGYKKRGLMFGFEHSIP
                     DATLPIFWSPGSNNWEPLIERA"
     gene            complement(13703..14473)
                     /locus_tag="VFF22_06695"
     CDS             complement(13703..14473)
                     /locus_tag="VFF22_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HZX16537.1"
                     /translation="MDHSVILRPFIDTLAWLIPALLLLGLLKTPWAKGHIGELLVRLF
                     AHWQLDQHSYRRLHNVTLNTPDGTTQIDHIFLSPYGIFVLETKNMSGWIFGSENQAQW
                     TQKLYKRTFKFQNPLRQNYKHLKALEATLGVNPEHLHSVITFVGGSSFKTAVPANVTQ
                     GIGFIRYIQSFQQPVFSTAEVDAMLHALQTGRRAPTLATQREHVQNLKRRSDPTAERQ
                     CPKCGSALVIRTVKSGAKAGSQFWGCSGFPRCRTMQSL"
     gene            complement(14860..15822)
                     /gene="egtD"
                     /locus_tag="VFF22_06700"
     CDS             complement(14860..15822)
                     /gene="egtD"
                     /locus_tag="VFF22_06700"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HZX16538.1"
                     /translation="MALANRVQQQALDPEQAALREEALTGFAASPKRLSPKFFYDHPG
                     SQLFELICRQPEYYLTRTEETILRLAATDIAALAGRNASLVELGSGASRKVRLLLEAL
                     RPARYLGVDISREFLHASTRRLAADYRWLDVHALCADFSQPIKLPAEALGERPLAFFP
                     GSSIGNFDPQQARALLRNLHQALPSGSGLLIGVDLVKPREVLERAYNDEAGVTALFNL
                     NLLERIRRELDSDIDSRRFQHRAFYNEAESRIEMHLVSPQAQQVRIEDQVFDFAAGET
                     LHTENSYKYTPASFRQLAADCGFASVSMWRDPAALFSVHFLRSE"
     gene            complement(15825..17108)
                     /gene="egtB"
                     /locus_tag="VFF22_06705"
     CDS             complement(15825..17108)
                     /gene="egtB"
                     /locus_tag="VFF22_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HZX16539.1"
                     /translation="MPNRAPSNDTSLVNLEHLRQRYHRVRHATERLCEPLEREDYVIQ
                     SMPDVSPPKWHLAHVTWFFEAFILQPFLRGYRPLDARYDHLFNSYYQTHGTPFERARR
                     GLLSRPTVAEVYAYRSYVDLAMEQLLSHLDSDIAGEVLPRVELGLEHEQQHQELLLMD
                     IKHILAQNPLRPAYRKDLKPGGTKAAALQWQTYPAGLRHIGHAGEDFAFDCERPRHRV
                     FIDEFQLASRPVTNAEYLDFIHDGGYHSSALWLADGWDHIRRAGWQAPLYWEREGDDW
                     LELTLAGPRELDREAPVCHLSYFEADAFATWAQARLPREEEWEVAAQDQSPWGNFVEN
                     DHLHPVAASAGEGPLQLYGDVWEWTASAFRPYPRFQPLAGSLGEYNGKFMSGQMVLRG
                     GSCVTPEDHIRPTYRNFFYPTMRWQFSGLRLAKEL"
     gene            complement(17384..18043)
                     /locus_tag="VFF22_06710"
     CDS             complement(17384..18043)
                     /locus_tag="VFF22_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16540.1"
                     /translation="MANLYADKQWAAFREQVIHLDGGVCVECGRGRADGVVLQVHHKH
                     YISGRKPWQYDHSDCETLCRGCHAREHGEIRPDSGWEYIGEDDLGDLAGNCELCGTAI
                     RHVHYVQHKHWEQMGVGTHCCDALTGTREATEARRRLGRLRRFLASSKWKRKNGRHTI
                     KHRGFKLAVVIEPAGCKLVIDNLQGKTRYESVMHAKEFVFDFIDSGKAAEYAKQRRLK
                     R"
     gene            complement(18125..18334)
                     /locus_tag="VFF22_06715"
                     /pseudo
     CDS             complement(18125..18334)
                     /locus_tag="VFF22_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003296742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4172 domain-containing protein"
     gene            18332..18718
                     /locus_tag="VFF22_06720"
                     /pseudo
     CDS             18332..18718
                     /locus_tag="VFF22_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249265.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            complement(18759..>19412)
                     /locus_tag="VFF22_06725"
     CDS             complement(18759..>19412)
                     /locus_tag="VFF22_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HZX16541.1"
                     /translation="GVEDACGLLGLPPHEKYAPTTEQVWKATRAYIPADRLQAQREHL
                     GWQLLTNYVVRNADCHAKNIALFYTSRADVAFTPVYDLVTTQAYPRFASNPPGLSVGG
                     RQTWTPGRSLETFFKAVLGIPPRQYAAMVEQLCESAIAVGREVIEAAKNEPAWRDVAK
                     HMVHAWNEGMETLRSPKAAPHFRGLDTAIEDAGFSGPRKPERSREVIGRSELQAKKR"
BASE COUNT         3897 a         5453 c         5666 g         4396 t
ORIGIN      
        1 cttgcccgcg atctcttgct gactcaaaag catcgcgggc aagcccgctc ctacggggaa
       61 ttgcgttcgc tttaggaggg cgcgcctggc tcaatcgtgc ttacccaacc catgcagcgc
      121 cagcttggcg tataggcctg cgcgggagat tcctaagcgt tcggcagcct gttggcggtt
      181 gccggcgctg gcctgcaggg catcgatcag cgcctggcgc tcggcgtgcg ccagctgttc
      241 tgccagggtt tgcgttactg tggaaggggg cagcggcgca atgctgtgcg ctgcgggcgt
      301 gaccggatca accagcaacg cccgcacgct ctgcgcatcc aggcgcccat cgacatccaa
      361 ttgcgcgcgt tccagcagat tgcgcaattc gcgcacgttg ccgcgccagg gttgtgcgct
      421 gagcagggcg agggcgtcgt cgctgacttc cagcggcgcc tgggtggagc ggttggcgat
      481 atcgtcgagc aggtgttcca gcaacgccgg aatgtcctcg cggcgctcgc gcagcggcgg
      541 tacacgcaag gccagcacgt taaggcggta gtagagatca tcgcggaact gcccggcggc
      601 gaccttggct tccaggtcga tatgcgtcgc ggcaatcacc cgcacattca gcggtttgac
      661 ctgattggag cccagcggct cgacctcctg ctcctgcaat acgcgcagca gcttggcctg
      721 cagcggcagc ggaaggtcgc cgatttcatc gagaaacagg gtgccgccgt tggctacctc
      781 gaacttgccg atgcgtgcct tgcgatccgc gccggtaaag gcgccgggcg cggtgccgaa
      841 cagctcagcc tcgaccaggg tttccggaat cgccgcgacg ttgaccgcga caaacggccc
      901 attggcccgt ggcgatagat tgtgaatgcc ctgggccagc agctccttgc cggtgccggt
      961 ttctccgcgc agcagcaccg tggcatccaa ttgtgcggcg cggcgggcct ggcgtttaac
     1021 ctcgctggcc gccgtgctgg cgccaataaa cccggcgatg gtgtaacgcg cacggcgcgc
     1081 cttggccagc tcgtgctggg tggcgaccag ttggttctgc agcacgttga acttggccat
     1141 cagcggcttg aggtggcggg cgcgatcaaa caatacaaag cccagcgcgc cgaccagggt
     1201 gccgtcctca tcgcgcagcg gaatgcgggt aaccacgaaa tgctcgctgc cgaaagccat
     1261 caggtcgagc atgcttggct ggccgctttc caccacttcg cgcagacggc tggccggcag
     1321 cacctcttcg atgggcttgc ccagcgcggt gcgtggatcg ctgatgccga ccttctcggc
     1381 atacttgtca ttgatccaga cgatccgtgc ctggcggttg accgcgatgg tgccctcgca
     1441 ttgcgcattc agatggtcga acagcagcgg catggccacg gcgcgaaaac gctcgggcga
     1501 aacgccgata atcagaccgt gttggtcgag gtcgtcgcgg gcaatggcca tgggcgcggt
     1561 acatccagaa tcaggtgcgg ccgattatgg gcggcgccgc gcgaaagcgg caaggaaccg
     1621 ctggcgagga tgcacggtcg atgttctata cctatctcaa ggagaacacc cgtggaccta
     1681 gttcgtattc tgatcgccat cctcttgccg ccgctcggcg tgtttctgca agtcggcttc
     1741 gccggcgcct tctggctcaa cattctgctg accctgcttg gctacatccc cggcatcgtg
     1801 catgcggtgt atatcatcgc caagcgttga gcgtgccgtc cttgccggtc tatggcgaca
     1861 agtcaggaaa ttggtcctga cgggctgacg gatgcggttc gagcctacgt ggcggttccg
     1921 attagccgct gcaccgccta tccagatcac tcaatccggt tcagccgcct cggcctcgcc
     1981 cagtaaatgt cgttggcgct caggctttag ttgtattgcc cgtctggcca aggtgtggcg
     2041 gttgaggcaa agtgagccat caaaccgcca tctatgcagc gccgggccat gttttttcga
     2101 ccgcgaacca aaccgggcag tgacaacagc gtagccgtcg acgatttgca ggatgtcggc
     2161 cagcgttcgt tgatccgcat gatctttgcc agtaccggac tgaccctcgg ggtgttctgc
     2221 gcgctgcaat ttatggccgg caactacctg ctggccagct tcgagctggt ggccaccggg
     2281 gcgctgctgt ggggcgcctg gtgcattgtt cgggtgcgca acctgctgcc ctggatctac
     2341 ctctacctgc tgccgacctt cagctttttg ctgtacatca tcgttattcc caacgcctcg
     2401 aaaaccgcct ttgtctgggt ctatagcatt cccgtgctgt cctacctgct gctcgggctc
     2461 aagcgtggca gcatgctggc gctgcccttc gtcattggcg cctgcctgct gtacctgtac
     2521 acctacccaa tccagcggga tgccgaaagc ctgatcgatc tgggcaatgc ggtgttctgc
     2581 ggagtgatga ttttggtctt cgtctacctc tacgaagccc gccgcgctgc tgcctaccag
     2641 cagctgcaac atatggcgcg caccgatgcg ctaaccggcg tggccagccg tggcagcttc
     2701 cagcagagcc tggaacagag catccgcgag gcgcagcgca gccgggccaa gctggtgctg
     2761 gtagtgctgg atgttgacca cttcaagatg gtcaacgatc aatacggcca cgatgccggc
     2821 gaccaggctc tgcggcatat ctgcaacagc cttagctcac gcctgcgcgc caccgacacc
     2881 cttggccgcc tgggtggcga ggagttcggc ctgctgctgc gcaataccga tggccagggt
     2941 gccgaaccgc tggtggaaac cctgcgcgag cagctgtgca gccagccgat gcgttacggt
     3001 gatgaggaca ttgccctgtc ggccaccttc ggcctggccg aatggcccgt cgatggcagc
     3061 accgccgagc agctgtatcg ctgcgccgat cagcgcctgt atcgcggcaa ggagctgggc
     3121 cgcaatcgcc tggttggccg tagcagcacc gtggataaat gcagcgccga gtaaacaccg
     3181 cttgcgcgcc gcataatcac gcagaaggct ctggttagga cgtctacact catatctgcc
     3241 catccataag ccgcagcagg catgcattcg acggcgctct tcgcggcaca acaggtcact
     3301 cagggacttt tcatgaccgt cctttccgcc tttctcaagc aggttctcaa gctctggcat
     3361 tcgcccgatc tagcgctgat cggtcagcgc cgcgagcggc gcatgcgcat gctcgccagc
     3421 ctggtactgc tgctggtcgg cagtttatgg ggcatcttct tcagcctgcg cggcgagtgg
     3481 ggcatcgtgc tgatggacct gctgatgatc ttctgcggcc ttggcgtctt tatcctgacc
     3541 ctgcgcaacc gcgtgcgcct ggccaacctg ctgctgttcg gcgtgatcat tctggtgatc
     3601 gtcggcacca ccttgctgat ggacccgcac accgctgctg cgccgcgcgc cacccacctg
     3661 tatttgctgc cgattgccgt ggccgcgcta atggcgtttc gcgatgaacg cctgtggctg
     3721 cgctacggcg ttgcgctgtt ctgcctgagt ttattcaccg tgctggcctc ttcaacctgg
     3781 agcccgacca gcgcctacca cctgccggat gacgtgcgca taaacggctc ctgggtgcag
     3841 ggtgtggcag ccatgctgat gctgtttgcc ctgctgcata tcctgcaaag cgatgccgcc
     3901 gagcgctcgc tgctcgaccg tgacctgcac gcggcgctgc gtgagcaaca gtttgtgttg
     3961 cactaccagc cgcaagtgaa cagcagcggc agggtcaccg gcgccgaagt gctgatccgc
     4021 tggcaacacc cgcaacgtgg gttgctgccg cctgccgagt ttatcggcca tgccgaaaaa
     4081 accgggctga ttattccgat gggccgctgg gtactggagc aggcctttac ccagctgcac
     4141 gcctggaagg acgacccgct gtaccgcgac atcagcctgg cggtaaacat cagccagaag
     4201 cagtttcgcc aggccagctt tgtcgcggag atgcttgggc tgatcgaaac ctacggcatc
     4261 gacgcgcggc ggctcgaact ggagctgacc gaaaccctga tcgtccacga catcgacgac
     4321 ctgatgggca agatggccgc actggtcgag cgtggtgtgc gcttctccct ggatgatttc
     4381 ggcaccggct tctcctccct cagccacctc aaacgcctgc cactcaacac cctgaaaatc
     4441 gaccgctcat ttatcagcga catcctcacc gacgccaaca gccaggccat cgtgcgtacc
     4501 gttatcgccc tggcgcaaag catgggcatg acggtgattg ccgaaggcgt ggaaacccag
     4561 gcgcagcagc agtttttgct gagtaacggc tgtgtgcagt ttcagggcta tctattcagc
     4621 aagccgctgc cgctgggggc gtttaccgcg tttgtcgagc ggcagaatgg ctaggcggct
     4681 gaacgcgctg tagtcgctga tccacgcata aatcgcttgc tcgcgcgctg cctttgtagg
     4741 agcgggccat gcccgcgatc accggcgctc taccggcaaa ccgcaatggt ggttacgcag
     4801 cgtccgaatt gcccggtgct gcccataacc cgtcccgcgc cactcactca ccctacaaaa
     4861 gtggcctgca taggtattag gcaggattaa gcgaagcgga tttatgtgcc atcaacttaa
     4921 tggcatttaa ctggccttta gtcagttata cgaaacggcg ataaatatcc tgggtttgta
     4981 caatttcata ttgctatgta accgttcatc ggctactgaa aaaactcatc aaacgtttct
     5041 gtagttgccg ttatcggagt aaatgcccgt tgcataaagg catgggtaac tagcgaaacg
     5101 gtagttcaca aaacttacag gagaactgtc atgactacta aaggtaataa caacccaggt
     5161 aacttcgcca atgaccgcga gaaagcctct gaggcaggta agaagggcgg ccagtcgtct
     5221 ggcggcaact ttgccaatga tcgtgaaaag gcatcggaag ccgggcgtaa aggcgggcag
     5281 caaagccatg gtggcggccg ccagtcttaa gctgcgtgtt tgtagcgtta ccgacgcgcc
     5341 gtaaatccag caagaggaag ccccaatgcg tgcggcacct gagtttaatc gaggcgaaat
     5401 acaacacgtc agtgttgtag aagtacaacc tgaagcgatg ttgatgacgc atctgaaaac
     5461 agctgctaaa tcccgtcttt atattcaagg ctgttatgaa cataaagagg gtgttgagta
     5521 cgccgaaggt aaaaccagtg cgccgcacat accggcagtc tgcgaaaaag ccttatagct
     5581 gcaataaggt ttggccgagc gtttaatgtc ggcacaactg gatgctgtta tttggctgta
     5641 tttgcagagt ctggaaataa ggtgatgttg tgagaggctg tgacggtaaa ggcggtagtt
     5701 ccttggctgc tacttctacc gtttacggcc tcaccatcat tcgctatgaa atagttgaaa
     5761 gtgactcagg ataagcacag ccgcgaaagg cctataccca agcctgtggt agcgccttta
     5821 tcgtcatggc gggtagcgcc caccttgagt gttttcggag gtgccttatg cctttgcatg
     5881 tgatcaattt cactggcccg atcaatgcct ccacctgcag ccagctgatc gaaaaagcct
     5941 cgttggcggt gcagcgcgat gcgtcaggct tgctcctgaa tctcgcgact atgggcggtg
     6001 agtgcagcta tggcttcacc atgtacaact tcctgatggc gttaccggtg cctgtgcata
     6061 cgcacaactt aggcacggtc gagtccatgg gcaatatcgt gtttttggcc gggcagcatc
     6121 gtacggcctg ccagcacagc aagtttctct tccacccctt tcactggcat ctgcaaggcg
     6181 cagtcgatca ttcgcgcatg tctgaatacg ccatgagcct ggattacgac ttgcagctgt
     6241 acgcgcgcat cgttgctgag cgcacggcgc atgccaggga aaaactggaa acagagaagt
     6301 acctgacagc cgccccgcgc attgtcgacc cgcagcaggc gctgttgtcc ggtctgattc
     6361 acgccatcga actgccgcca atcagcgcgg aggcggtgag cagctttatc cacacctagc
     6421 cctccgtggc caaacagcgc ggcttagccg acccctcacc gcaactaccg tttgcccgag
     6481 aaccggaact tattcgccag cgctgcgctc cccttttaga cacagccaac gcgagcctgg
     6541 cgggtctgcc aaccctcgcc aatcaccggg ccagggtcgc ccagcgatga ggaacgaccg
     6601 tgaatgcaac cacccaccct acctaccggt gccgaccggg tccgctgcac gcaaccctgc
     6661 tggccgggac cgtgccgctg tttctcggcg gcctgctgag tgatatcgcc tactttcgca
     6721 gctatcagat tcagtggagc aacttcgcct cctggctgat cgccggtgct ctagtgtttt
     6781 gcggtttggc gctgctgttc gccctcgtca atctgctgcg cgcccgccct cgctccgggc
     6841 ggccgctggt ttactttttg ctcctgctgg cgacctgggt actgggtttt atcaatgccc
     6901 ttgaacacgc caaagacgcc tgggcaacca tgccggccgg cctggtgctg tcggtgatcg
     6961 tcaccgtgct ggcctgcgtg acgacctgga tcggcctcag caacctgcgt acaggagatg
     7021 ccgcatgaac tccatccgca cagctctgac cctgacactg gcgttgctcc tcagcgcatg
     7081 cgatggcgag cagcagggga gcgctcaaca ctacggcccc gcgcccacgc tgccggaacc
     7141 cgaacggggc atgctgccca gcatgaagat tgccgagccg cagccgtggg gggatcaggt
     7201 gccgagcgtg ccggaaaact tcagcgttac ggccatcgcc accgacctga aaatcccccg
     7261 gcaaaccctc gtgctgccca acggcgacat cctggtggcg gaaggccgag gtggcaatgc
     7321 cgcgaagctc aaaccgaaag atgtgattgc cggctatatc aaagccaagg gcaacaccgc
     7381 agtcgagggc ggtgatcgcc tgaccttgtt gcgcgacgcc gatggcgacg gccgttatga
     7441 gctgcagcag gtgttcgccg acaacctcaa cgcgccctac ggcctggcct tttacgaggg
     7501 caatctgtac gtcgccaatc aggatgcatt ggtgcgtttc gactatcagg acggccagac
     7561 cgaggccagc ggcccgccaa ccaaggtcat cgacctgcca tccgagatca accatcactg
     7621 gaccaaggcg ctgaccatca gccccgacgg gcgctttctg tatgtcggca tcggctccaa
     7681 cagcaatatc accgagcgcg gcatggaagc cgaagtcgac cgggcgctgg tctggcagat
     7741 caatgctgaa acaggcgcct acaaacccta tgcaaccggc ctgcgcaacc ccacggcgct
     7801 ggcgattcag cccgagaccg ggctgttgtg ggccgtggtc aatgagcgcg acgaactggg
     7861 cgcagacctg gtgccggatt acctgacctc ggtacgcgaa ggtggctttt acggctggcc
     7921 ctacagctac tggggcaaaa acatcgaccc gcgcgtgcgc ccgcagaagc cggaaaaagt
     7981 cgccagcgcc atcaccccgg actacgccct cggcgcccac gtcgccgccc tcggcctgga
     8041 tttctccgtc cccgccatgg gcgagaactt cagcgaaggc gtgtttatcg gcgagcacgg
     8101 cagctggaac cgcaaaacgc cggtcggcta caaagtgatc tttgtgccgt tccgcgacgg
     8161 caaaccttcc gggccaccgg tcgacttcgc caccggcttt cgcaccgaag caggcaaaac
     8221 ccgcggccgc ccagtaggcg taaccgtcga cccgcgcggc gcgctgattg ttgcggatga
     8281 tttggcgaat acggtatggc ggataagtcg tttgcagtga gtggttttgc atgacgttgt
     8341 cgagcaatca gtcccaaaaa gggaacgata ggtccctttt tgggacctat cagactgtaa
     8401 ttagtagagc cggtttaaaa agccatgatt gattctcgga gaaaaccgtg gtctgtcccc
     8461 tgttactgcc tgctgttact gcttttcgta ccgagatatg gtcaggctgt gctttcctat
     8521 gtatggcggg gtgttgacac aggatggtga ctctacggct tccctcgcta tggattttgg
     8581 cagcgcaggg cggcgggtca cgtggtaaat atgtcagtta ttctgggtag ttaaagcaag
     8641 tgaaggcagc caaatattta atcttgaaat agttatatag cgcgcgcctg ttccgcggta
     8701 aatttttata aaatcccccc accgagagtg aattcggcgt tctgctttca tagcatttgc
     8761 cttgttttga aactctagag caataacaca atcagaaagt tgaacggttg tttcgagttt
     8821 gattgccttt acgtattgga tatcgggaac gccgactcta ggaatcaaaa tcacccagcc
     8881 ttttgttcta cctttagcga cagttgaggt cttagaaagc tctagtgttg aaccggaagt
     8941 tactacctcc cgtatatctg tggagtgcag aaagggcact ccagtcggat cactaacatt
     9001 taaatgaaca gggaggctcc cgcgtaggat agttgtgtga ataacgacgc tgctaggcct
     9061 catttcttga agtgaagggt gatgagcgcc tggagaaatt tggaggacgg atgcctgtac
     9121 ccttgctccg tgaaacgtac atcgcttcag ttcagcaatt tgttgaaact ggtaatgctc
     9181 ctccaatgtc tggcgagcaa aatggtctgt ctcggaatag agcagagatt ccggtgctat
     9241 gacaattgcg cctaatttcg gctgaaacag ctcaaagctt cttagcaaat gagccatggc
     9301 gatgcttccc ttcatctcct tgccggcaaa tcggatgtcg acagacttcc ttttgccgtg
     9361 gctgaaggga gggttgagta ctagtaggtc tacggagttt cggtttggta gtacttcatc
     9421 aggctcattg tggagcaagt cgcctaccgc caaatgccat tcaggccgtc ttaagcgcag
     9481 ctgggatata gcatccacgt ctcggtctat accgaaggat ttgactggcc cgaaaactgt
     9541 gttcgcggca tcaagtagcc gaccggaccc gcaagtcgaa tccgcacaag ctttgggtga
     9601 aaagggaaag ctcgctcgct ccaacgcgct cattgcgacg ttctctggag tgtagtacct
     9661 atccatatca ataggcatgg aagtcttcaa agtcataaat gtgaacgatc cccacgccag
     9721 tggtccgtcc acttttctta accggatagt caaacatgat ttccgttgtc tcggcgtatt
     9781 ttatagcgaa gagtatggag ccaatggcgt gcggtttggt gcctacgaga gccaagtata
     9841 tatagtgatc tgggtaatct ctacggatct tgctcagtga attaaatgcc tcaaaggggc
     9901 agcttgcacg ggcgtatctg acctctgagt gagcccggta ctcgtccaaa aacattcgat
     9961 tgcagccaac ggtgaacgct ggatattcga gttggaagcc gggggcaccc acgactggga
    10021 taacttttgg ggtcgggtct agctgggatg caaggtgttc aggtcgatta ccctcaaaac
    10081 ctaacatcgc aatgaataag catttatctt cgtctaccgg ccctgctaat tgtacgaagc
    10141 cgggcaaagg cgctaaccct tcaaatgtaa cgcttaaatc gaaaatggcg gaagatgctg
    10201 ggcttgggtg aggcgtataa gactctggtt cagcatataa aactcgtgtc cgaatctttt
    10261 gcgaatatgc actctttaat agtggagccc agatattgtg ggctaatccg gtaacgtcta
    10321 tcaatatttt cttcgaaaca aagatgctgt tgattgcatc ttcgtctctg agtgatacgt
    10381 ttttttccgt tccgttgtga gtgacaagaa tgctttcctt gtcaattttc ttgatcaaaa
    10441 taatgcgctt tggatcggat gtatgtatct gctcccacaa aggaagtctg tctctgttct
    10501 cagctgattc ggcagccgat attattagtt cgtcagcatt tatatcagct atggttatat
    10561 ttttgaaacg ttttgtattt agcgatggtg taggcattgt tgtcatttaa aatccccagc
    10621 gtcgtagaat gccgagaata atgcgagctg ctcaggaagt tcttcttcag atgtttgggg
    10681 tctatcatct aggagtgtgg aaatggcccg ggaaggccga ttttctaaca cgcttataag
    10741 ggtagaggct tcaattgtca ttcgcctctt cttgcggtgt gatatctcga aaaatgcact
    10801 gaagattcga tgcagacgat attcatcatc gcgaagctct gagctgctag tggacttagt
    10861 tctagggtca gattcgaaag ctaagtgggc aacgccctct tggagtaatt catgaatcct
    10921 gctagcttcc gcagggcttc cagagagtac aaatgatgta acttcgggag tcttcccact
    10981 cggggctcga gccagctcaa aaaaaacctt accaattgcg agaacaatcc gtttaagttg
    11041 aactccgtgg tctgcaagcc cttcaagttg gttcagtcgt ctttttccga cttctctcgc
    11101 tgcaagtgtt tgagatttcg gagatattgt taatagtggc tgctttgatg gctcttcatt
    11161 tagttggtag ctgatagctg tgtcgatcaa ctctagaaaa tacctgatat tgccggccgc
    11221 taatgaaaga agagttcttt ctccgcagta gtactttcta attcgtgcgc ctttacgacc
    11281 tttagatagc cagaaaaggc tagcgtaacc atgatttcca agccgggttt tccactcggt
    11341 ttcgttccgg taccagtcat cggcgagatc ttttaatgat ccgccctcac tttcctgcca
    11401 atattttaag aaatagattt cgcctttcga cttctgctta aagtactcaa aatgctggct
    11461 gccatctagt tcggaaagaa tattttctgc tatgcggtct gcacctaaaa cgattgcctc
    11521 ttctgatagg gatagctctt gcatgaactc gcggaggcta ttagggactt tagacccaac
    11581 gctttgagcg tatcttagtc ttaattcagc tacggctttc gcgaaaagct cgaagccttc
    11641 atccgcaatc tcaatttcga gacagtcgtc gggcactctg agtaagtctt ggccatcaag
    11701 tgtctgtcta tttcgtagtc catttttacg gacacccatt ttgtaagaaa gaggcggctc
    11761 agcatgcttt atatatgtgt tgattatggc ttgttgatag tcaagtagat tctcatattc
    11821 atcaatgcag caaagaatca ctctgtcgcc aagcaaacct gctgatttta atacctcaac
    11881 gaaggtacgc agcggggctt cagccatgga aagcattatc tccatgtctt tgagcggatt
    11941 gttcacttgc agttggagag atgatatcgc ttttttaatt gagctcttga gttggtccag
    12001 tgtcccgcat tcatccaggc caagcgcatt agatatttcg ctgagacttt cttcaggaag
    12061 ttttaatccc agtctaactt caagccaaga tgtgagatta gctagttcgc tgcagacaat
    12121 gagattgaag taatgagcaa aaagttttga ccaacgttct tcgccaacac cacttcctga
    12181 gaatgctcgt acacgattct tgttcatgcg aacgagaaca ccgagatatt cttgatcggc
    12241 gaaggtctgg ccttctcctt caattctctc cagcattgag tcatatcgca gcgactggag
    12301 tgccgtagtt ttccctgttc cgcgaccgcc aacgagaaaa catgggcgca tgacttcaag
    12361 tttgccaaga taggtcggtc ttacgaacag atccttgaag ttagcagagg gccactccgc
    12421 tcggttgtcg ccgaatagct gccctaagac aaaatttgga ttaagctcgc tcaattagtg
    12481 gctcccagtt attcgatccc ggcgaccaaa atattggtag tgtagcatcg ggtatagagt
    12541 gttcaaaacc aaacataagg cctcgttttt tatacccgaa cattaagtag ttcgcgttgt
    12601 tagctatata aggttcagcc ggcgttaggc gtgtagagta tttagataaa aatcttagaa
    12661 tcggtaaatt catgcttggg tttgagtcaa gaacaccctg taacgttgac tttttctcga
    12721 tcacgtgagt tgcgcatact gcgacttttg gtgcgcggat atttatattg tctaagccga
    12781 agtcagttgt tactaatgtg acatatattg ccgtgaagcc gctgatgtcg cttgcttttt
    12841 taaaagatct tcccgctttg tcagcagttt gccatgtata taaaaactga tcgcctgagc
    12901 caacgaagtc atctatgaaa acgacagtgt tccccgcgta agcgtgctga agtgcttcat
    12961 ctgtatccaa gatgaagctg tcatttaagt ggagcagttg acgtgcttta cggcaaagca
    13021 agtagcctga atcggtgggg tttgggcgtt cacccttcac tggcgtaagg attgccgaac
    13081 ctagggcagc aacgagatcg tttccgagtg gtgctgaagt gcccttaggc attccgtccc
    13141 caattgaatt gtaagaggcg actaacaaag aatctgttaa taccctgtta tagaaagtga
    13201 acttatctaa gagaaatgcg gccacgtagg catcttgctc gtcaaaattc atcagccagg
    13261 cgcgggggcg cagaattggc tcagacggcc atagcccagc acgttttagt atgtcgcact
    13321 tagctagaat ctcttggatt tgggtgtctg cacgcaaggg tgatctccat ttcttctcgc
    13381 tgcagttcga ggtgcaccgc tacaaattct gatgactgca tccataccca gaagcggtga
    13441 aataatactt agtggattca gaagtcttat agctagtgtt agctggagta attcttgcat
    13501 tttcaagcag cttgattttc aggctagcaa aatgctttct ggccgtctat gagcgtctat
    13561 ggctggcttc ccgcattata agctgaagct actcaacaaa tgattggaag gtagcgggag
    13621 gcggtagcac aggaaaaagg gaaaaagggg acagatttat ttgttgccca tggactcttt
    13681 gaccttttca ttgctctgtc actcataggc tctgcatcgt ccgacacctc ggaaaccccg
    13741 aacaccccca aaactgcgac cccgccttcg cccccgactt cacggtacga ataaccagag
    13801 cactgccaca tttcgggcat tgccgctcgg ctgtcggatc acttctacgt ttaaggtttt
    13861 gcacatgctc gcgctgagtg gcgagggtcg gcgcgcggcg gccggtttgc agagcgtgca
    13921 gcatggcgtc gacttcagct gtgctaaaca ccggctgctg gaatgactgg atatagcgga
    13981 taaagccgat gccctgggtg acgttggccg gcactgcggt tttaaagctg ctgccgccga
    14041 caaaggtgat taccgagtgt agatgctcgg gatttacgcc cagggtggct tccagggctt
    14101 taaggtgttt gtagttctgc cgcagtgggt tctggaattt gaaggtgcgt ttgtagagct
    14161 tctgcgtcca ctgcgcttgg ttctcgctgc cgaaaatcca gccgctcata ttcttcgttt
    14221 ccagcacaaa gatgccgtag ggcgagagga agatatggtc gatctgcgtg gtgccgtcgg
    14281 gcgtattgag ggtgacgttg tgcaggcggc ggtagctgtg ttggtccagc tgccaatggg
    14341 cgaataagcg caccagcagc tcgccaatat ggcccttggc ccagggcgtt ttgagcaggc
    14401 cgagcagcag cagggcgggg atcagccagg ccagcgtatc gataaatggc cgcaggataa
    14461 cgctgtggtc catgccttct tccttgagca atgccgcttg agtggcgccc gtaagccgag
    14521 cgagcaggcg tgtcggaact ccagatgcat gacactgctc cggataatgg ccaattgctt
    14581 tcaaaggctc aaggggctag cgaccaatgt cgagtaattg ctcgcttggc gtgcctggct
    14641 tagcgcggtg cgcgctagga atgcaggctc gcagcggttc tgcggcgcgc ttggtgctga
    14701 gggtgtttgc gctggcctga agggcagggc agcgcgcatg gatggggctg gctgatcgtg
    14761 agccgattag cgcgcctatt cggctcactg cataagccgc tgctgcgggc gattcggctt
    14821 atatcgccac cgagcgtctg cccagtggcg gcggggcggc tactcgctgc gcagaaagtg
    14881 cacgctaaac agcgccgccg ggtcgcgcca catcgacacc gaggcaaagc cgcagtcggc
    14941 agccagctga cgaaagctgg ccggggtgta tttgtaggag ttttccgtgt gcagggtttc
    15001 gccggcggcg aagtcgaaca cttgatcctc gatgcgcacc tgctgcgcct gcgggctgac
    15061 caggtgcatc tcgatgcgtg attcggcttc gttatagaag gcccggtgct ggaagcggcg
    15121 cgagtcgatg tcgctgtcca gttcgcggcg gatgcgctcc agcaggttga ggttgaacag
    15181 ggcggttacc ccggcctcgt cgttgtaggc gcgttcgagc acttcacgcg gcttgaccag
    15241 gtctacgccg atcagcaggc cgctgccgct cggcaaggct tgatgcaggt tacgcagcaa
    15301 ggcgcgggct tgctgggggt cgaagttgcc gatgctggag ccggggaaga aggccagcgg
    15361 gcgttcgccc agcgcctcgg cgggcagttt tatcggctgg ctgaagtcgg cgcacagggc
    15421 gtgtacgtcc agccagcggt agtcggctgc caggcggcgg gtgctggcgt ggaggaattc
    15481 gcgggagatg tccacgccca gatagcgcgc ggggcgcagg gcttcgagca gcaggcgaac
    15541 cttgcggctg gcgccgctgc ccagttccac caggctggcg ttgcgcccgg ccagcgcggc
    15601 aatgtcggtc gcggccaggc gcaggatggt ttcctcggtg cgggtgaggt agtactcggg
    15661 ttgccggcag atcagctcga acagctgcga gccggggtga tcgtaaaaga acttgggcga
    15721 caggcgtttg gggctggcgg cgaaacccgt cagggcttct tcacgcagtg ctgcctgctc
    15781 gggatctagg gcctgttgct gaacgcggtt tgccaatgcc atggtcagag ctccttggcc
    15841 aggcgcaggc cggagaactg ccagcgcatg gtggggtaga agaagttgcg ataggtgggg
    15901 cgtatgtggt cttcgggggt tacgcagctg ccgccgcgca gcaccatctg cccggacata
    15961 aacttgccgt tgtattcacc aaggctgccg gccagtggtt gaaagcgcgg gtatgggcga
    16021 aaggcgctgg cggtccactc ccatacatcg ccgtagagtt gcagcgggcc ttcgccggcg
    16081 ctggcggcca ccgggtgcaa gtggtcgttc tcgacgaagt tgccccaggg cgactggtcc
    16141 tgggcggcga cttcccattc ttcttcgcgg ggcagccgtg cctgcgccca ggtggcgaag
    16201 gcatcggcct cgaagtaact gaggtggcag accggcgcct cgcggtccag ctcgcgcggg
    16261 ccggccaggg tcagctccag ccagtcgtcg ccctcgcgct cccagtacag cggcgcttgc
    16321 cagccggcgc ggcggatatg gtcccagcca tcggccagcc acaaggcgct gctgtggtag
    16381 ccgccgtcat ggataaagtc gaggtattcg gcattggtca ctggccggct ggccagctgg
    16441 aattcatcga taaagacgcg atgccgcggg cgctcgcagt cgaaggcaaa gtcctcgccg
    16501 gcatggccaa tatggcgcaa gccggcggga taggtttgcc actgcagggc ggccgccttt
    16561 gtgccgccgg gcttgaggtc cttgcgatag gcagggcgca gcgggttttg cgcgaggatg
    16621 tgtttgatgt ccatcagcag cagctcctgg tgctgctgct catgctccag gcccagctcg
    16681 acacgtggca gcacctcgcc ggcaatgtcg ctgtccaggt ggctcagcag ttgctccatg
    16741 gccaggtcga catagctgcg gtaggcgtac acctcggcca cggtcggccg cgacagcaag
    16801 ccccggcgcg ctcgctcgaa gggcgtgccg tgggtctggt aataggagtt gaacaggtgg
    16861 tcgtagcgcg catccagcgg gcggtagccg cgcaggaacg gctgcaggat gaacgcctcg
    16921 aagaaccagg tgacgtgcgc caggtgccac ttcggcgggc tgacgtccgg catgctctgg
    16981 atcacatagt cctcacgctc cagcggctcg cacaggcgct cggtggcgtg gcgtacgcgg
    17041 tgatagcgtt ggcgaaggtg ttcgaggttt accagagacg tgtcgttgct gggggcacgg
    17101 ttcggcatgt cgcgcatctc tactggcgtc gcagggaagg cgcgacctgt cattaagccg
    17161 accggccgag ggcagaaaag gttcagggaa tttgttgttt aaacgtgcga cggctattgg
    17221 gcgcgtggtt tggcgggtgc tagttggtgg gctggcgtcc ggtgttgcgc cgactaacga
    17281 gcctgtttgg ccaactgtcc agtccagccc aggcttggtt gttgatccgg cgctagcggt
    17341 caggccgcgc cctacccatg cagcacggcg gcaattgccc acattagcgt ttgagccggc
    17401 gttgcttggc gtattcggct gctttgccgc tatcaatgaa gtcgaatacg aattcttttg
    17461 catgcataac gctttcgtag cgtgttttcc cctgcagatt atcaataacc agcttgcagc
    17521 cagccggctc gataaccacg gccaatttga agcctcggtg ttttattgtg tggcggccgt
    17581 ttttgcgttt ccacttggac gaggcgagaa accgtctgag ccgacctagg cgtcgtcggg
    17641 cctctgtcgc ctctcgcgtg ccggttagcg catcgcagca gtgagtgcca acgcccatct
    17701 gctcccagtg cttgtgctgc acgtagtgca catggcggat cgctgtaccg cacagctcac
    17761 agttcccggc gaggtcgccc agatcatcct cgccaatgta ctcccaccca gagtcgggcc
    17821 gtatttcccc atgctcgcgt gcatggcagc cccggcagag cgtttcacaa tcagaatggt
    17881 cgtactgcca aggcttgcgc ccgctgatgt agtgcttgtg gtgaacttgc aggacgacgc
    17941 catccgcccg cccacggcca cactcaacgc aaacgccacc atccagatgt attacctgct
    18001 ctctgaatgc ggcccactgc ttgtcggcat aaagattcgc catccctagc tctccagcgc
    18061 ctgtccttgt cagcccttgc gggcttagcg tcgcaactcc agtgcatcgc ttgttcagat
    18121 cagcctagct gcctggcgcg ctggttgcgt agcagatttg gtagcgggtg ctacgaccgc
    18181 caccgggtag gcgggtgatg cagccttttt ccaggaggtc gctgagatgg cgggttgcgg
    18241 tggctttgga aaccttagcc acggcctgat attgagcggc gctgatgccg ccctcaaagc
    18301 cgcgctcgcc gccatcgagc aggcggttaa gcagattgtt tccggtcgcc tcgaccgacc
    18361 tcccgtgcac tttgaagccc cgcccccgtg ccgggctgga agagcaactg gccgacttcc
    18421 tctcctggtt cgagctcagc cgcaacgatg ccggcctcga tccattgctg cgcgccggca
    18481 ttgcccattg cacccctttg atgacggtaa cggtcgcctt acccgcgcga tcaccgacct
    18541 agcattggct cagagcgaac agcaggccat ccgtttttac gccatgtcag cgagcatcct
    18601 caacgaccgc gccggttatt accgcatcct cgaagccagc cagaaaggca cgctggacat
    18661 cactgcctgg ctgcaatggt tcctcgcaac gctgctcaac agcttggaac aagcccttcg
    18721 ggggcgtgat ctgcagccgt tggccctgcg ctataaattc agcgcttctt tgcctgcaac
    18781 tcagagcggc caatcacctc tcggctgcgt tctggtttgc gcggccctga gaagccggca
    18841 tcttcaatgg cggtatccaa gccgcgaaag tgcggggcgg ctttggggct gcgcaatgtc
    18901 tccataccct cgttccaggc atgcaccatg tgtttggcca catcgcgcca ggcgggctcg
    18961 tttttcgcgg cttcgatcac ttctctgccg accgctatgg cggactcgca gagttgctcg
    19021 accatggcgg catactggcg cggtggaata cctaggacgg ctttgaaaaa ggtttccagc
    19081 gaacggcccg gtgtccaggt ttgtcttccg cctacggaca ggccaggtgg gttgctggcg
    19141 aagcgcgggt aggcctgagt tgtcaccaag tcatagaccg gagtaaaggc cacgtcagca
    19201 cgcgaggtgt aaaacagcgc gatgttcttc gcgtggcagt cagcattgcg caccacatag
    19261 ttggtaagca gctgccagcc taggtgctcg cgttgcgctt gcagacgatc agcggggata
    19321 taggcccgcg tggccttcca cacctgctcg gtggtggggg cgtacttttc atgtggcggc
    19381 aaacccagta gtccacaggc gtcttcgact cc
//
LOCUS       DASRUM010000098         3874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_891219, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000098 DASRUM010000000
VERSION     DASRUM010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891219"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /gene="rnpB"
                     /locus_tag="VFF22_06730"
     ncRNA           complement(<1..170)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFF22_06730"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(212..1117)
                     /gene="rsmI"
                     /locus_tag="VFF22_06735"
     CDS             complement(212..1117)
                     /gene="rsmI"
                     /locus_tag="VFF22_06735"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017340693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HZX16542.1"
                     /translation="MRALLILRGCSVTTSAAANSPLGSLYVVATPIGNLDDISARALT
                     ILRSVALIAAEDTRHSARLMQHFGIGTPLAACHEHNERDQGGRFLARLLAGDDVALIS
                     DAGTPLISDPGYHLVRQARAAGIAVVPVPGACALIAALSAAGLPSDRFIFEGFLPAKA
                     AGRRARLEQVKEESRTLIFYEAPHRILECLQDMQAVFGDERPALLARELTKTFETLKG
                     MPLAELAAWVAADSNQQRGECVVLVAGWQAPEGEEAVSAEAMRVLNLLLAEMPLKRAA
                     ALAAEITGVRKNLLYQVALEQQKDV"
     gene            1222..3033
                     /locus_tag="VFF22_06740"
     CDS             1222..3033
                     /locus_tag="VFF22_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HZX16543.1"
                     /translation="MIARLRALSILCLAGLLAACASQPSSTLGELPRTPQASIEQLLQ
                     QAAESPTEQAAALRLSAADLAYKQKDLTRASSILEQVQLESLNPAQQIYASTLRAELD
                     MSRNNPKAALSALSHPSMERLGELPVEQQVRSQLVRANALQATGQTLAAARERAYIAP
                     LLSGASAAHNQEAIWTLVSSLPASELQASGDADLDGWLELARASKSAGPLEQQQTAID
                     AWIAANPQHPAAQQLPQALVELKKLSSQPLTEIALLLPQDGQLASVARALRDGFLAAH
                     YQAQQAGQNPPNIQIFDSSRIGSMDAFYAQAQAAGVQLVIGPLEKNLVSQLSSREQLP
                     ITTLALNYSEGSQEGPAQLFQFGLAAEDEAREVARRAWADGMRRAVALVPNGEWGDRV
                     LAAFRQSWQAQGGTLIAAEHVDQPVELARQIADLFQLRESEARSKRLQNTLGTSVAAA
                     PSRRRDVDFIFLAATPQQAQQIKPTLAFQYAGDVPVYATSHLFTGTYNQAQYIDLNGI
                     MFCETPWLLNVNDPLRQQASTQWPQAGGSLGRLYAMGADAFQLAPRLNQLKALPETRL
                     QGLSGNLSLNPAQRIERQLPWAEFRDGQVQPLPDSIN"
     gene            3030..3404
                     /locus_tag="VFF22_06745"
     CDS             3030..3404
                     /locus_tag="VFF22_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HZX16544.1"
                     /translation="MSERISTQQRGDAAEAQARQHLEQHGLRLLAQNWRCRLGELDLV
                     MLDADTVVFVEVRYRRHSAWGGAVESVDARKREKLSRAAQHFLQQESRWAKYPCRFDV
                     IAINADSQAPAQLNWIQNAFDT"
     gene            3435..>3874
                     /locus_tag="VFF22_06750"
     CDS             3435..>3874
                     /locus_tag="VFF22_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HZX16545.1"
                     /translation="MDMQARIRHLFQASIETKQQAMEVLIPFIEQGSQAMVQALLSEG
                     KILSCGNGGSAGDAQHFSSELLNRFERERPSLPAIALTTDSSTITSIANDYSYNEIFS
                     KQIRALGQPGDVLLAISTSGNSANVIQAIQAAHDREMTVVALTGR"
BASE COUNT          807 a         1386 c         1075 g          606 t
ORIGIN      
        1 caccttttca cccttaccgg caccgaagcg cttaggcggt tattttctgt ggcactttcc
       61 gtaggctcac gcctcccagg cgttacctgg cactccgccc tatggagccc ggactttcct
      121 cccccgtatt acgcagaacg caacacggca gcgactgtcc gatcgactct ccgcgggcaa
      181 ggttaacggc ccgcgcccta tagaacaagg tttaaacgtc tttctgctgc tccagcgcca
      241 cctgatagag caagttcttg cgcaccccag tgatctccgc cgccagggcc gccgcgcgtt
      301 tcagcggcat ttcggccaag agcagattca acacacgcat ggcttcggcg cttacggcct
      361 cttcaccctc cggagcctgc caacccgcga ccagcaccac acattcgcca cgctgctgat
      421 tgctgtcagc agccacccaa gcggccagtt cagccagcgg catgcccttg agcgtctcga
      481 aggttttggt cagctcacgc gccagcaacg ccgggcgctc gtcgccaaac accgcctgca
      541 tatcctgcag acactccaga atgcggtgcg gcgcttcata gaagatcagc gtgcgcgact
      601 cttccttcac ctgctcaagc cgcgcgcgac gtccagcagc tttggcgggc agaaaacctt
      661 cgaaaataaa acgatccgac ggcaagcccg cagcagacag cgcagcaatc aatgcgcaag
      721 cccccggcac gggcaccacc gcaatgccgg cagcccgcgc ctggcgcacc aggtgataac
      781 cgggatcgga aatcagcggc gtaccggcat cggagatcag cgccacatca tcacccgcca
      841 gcaatcgtgc gagaaagcgc ccaccctgat cgcgctcgtt gtgctcatgg caggccgcca
      901 gcggcgtgcc gatgccaaag tgctgcatca gccgcgcgga atggcgggtg tcttcggcgg
      961 caatcagggc cacgctgcgc aggatggtta gtgctcgcgc gctgatgtca tccaggttgc
     1021 caatgggcgt agcaaccaca taaagactgc ccaaaggcga attcgcagca gcggaagtgg
     1081 tcacagaaca gcctctcaaa atcagcaaag cgcgcattgt agcccgtttc caaccttcga
     1141 catcgcgtca cccggcgggc ttgggtacaa ttcaacgctc aatttgatca agtatcagga
     1201 acgccctaca tgcccacccg cctgatcgcc cgcctgcgcg ccctttccat cctctgcctt
     1261 gccggcctgc tagccgcttg cgccagccag ccctcctcga ccctgggcga gctgccgcgc
     1321 acaccgcaag ccagcatcga acaactgctg cagcaagcgg cagaaagccc gaccgagcag
     1381 gctgcggcgc tgcgcctgtc ggcggctgac ctggcctata agcagaaaga cctgactcgc
     1441 gccagcagca ttcttgagca ggtgcagctg gaaagcctga atccggccca gcagatctac
     1501 gccagcaccc tgcgcgccga gctggacatg agccgcaaca accccaaagc agccctgagc
     1561 gccctcagcc acccgagcat ggaacgcctc ggcgagctgc cggttgaaca gcaagtacgc
     1621 agccagctgg tgcgcgccaa cgccctgcaa gccaccgggc aaaccttggc cgccgcccgc
     1681 gaacgcgcct atatagcccc actgctcagc ggcgccagcg ctgcgcacaa ccaggaagcc
     1741 atctggaccc tggtttccag cctgcccgcg agcgaactgc aagccagcgg cgatgccgac
     1801 ctcgacggtt ggctggagtt ggcccgcgcc tccaagagcg ccggccccct ggaacaacag
     1861 caaaccgcta ttgatgcctg gatcgcagca aacccgcagc acccagccgc acaacaactg
     1921 ccgcaggcgc tggtcgagct gaaaaagctt tccagccaac cgctgactga gattgccctg
     1981 ctgctgccac aggatggcca gctggccagc gtggcccgcg ccctgcgcga cggcttcctg
     2041 gccgcgcact accaggcgca acaagccgga cagaacccac cgaacattca gattttcgac
     2101 agctcgcgca tcggctcaat ggacgctttc tacgcccagg cccaggccgc tggcgtgcag
     2161 ctggtgatcg gcccgctgga aaagaatctg gtcagccaac tgagcagccg tgaacaactg
     2221 ccgattacca ccctcgccct gaactacagc gaaggcagcc aggaaggccc ggcgcagctg
     2281 ttccagtttg gcctcgcagc cgaggacgaa gcccgcgaag tcgcacgccg cgcctgggcc
     2341 gatggcatgc gccgcgccgt agcgctggtg cccaacggcg aatggggcga ccgcgtgctc
     2401 gcggccttcc gccagagctg gcaggcccag ggcggcacct tgatcgccgc cgaacatgtt
     2461 gatcagccgg tagaactggc ccgacagatc gccgacctgt tccagctgcg cgaaagcgaa
     2521 gcccgcagca agcgcctgca aaacaccctg ggcacctcgg ttgctgctgc cccgtcgcgg
     2581 cgccgcgatg tcgacttcat cttcctcgcc gccacgccgc agcaggccca gcagatcaag
     2641 ccgactctgg ccttccagta cgcaggtgat gtgccggtgt atgccacttc acacctgttt
     2701 accggcacct acaaccaggc gcagtacatc gacctcaacg gcatcatgtt ctgcgaaaca
     2761 ccttggctgc tgaacgtcaa cgacccgctg cgccagcagg ccagcacgca atggccgcag
     2821 gccggcggta gcctcggccg cctctacgcc atgggcgccg atgccttcca gctggcgcca
     2881 cgcctgaacc agctcaaggc cctgcccgag acccgcctgc aaggcctgtc cggcaacctg
     2941 agcctgaacc cggcccagcg tatcgagcgg caactgccct gggcagagtt ccgtgatggc
     3001 caggtgcagc cgctgcccga cagcatcaat tgagcgagcg catcagcacg caacaacgcg
     3061 gagacgctgc cgaagcccag gcccgacagc atctcgaaca gcacggtctg cgcctgctgg
     3121 cgcagaactg gcgctgtcgc ctcggcgagc tcgatctggt catgctcgac gccgatacag
     3181 tagtattcgt agaagttcgc tatcggcgcc atagcgcctg gggcggcgca gttgagagcg
     3241 tggatgcacg caaacgtgag aaactcagcc gcgccgctca gcacttcctg caacaagaat
     3301 cacgctgggc caagtaccca tgccgttttg atgtgattgc cataaacgcc gacagccaag
     3361 ccccagcgca actgaactgg attcaaaacg cctttgatac ctgaacagaa gccacactga
     3421 aggttatatc tccaatggac atgcaagccc gtattcgcca cctgttccaa gccagtattg
     3481 aaaccaagca gcaggccatg gaagtgctca tcccctttat cgagcaaggc agccaggcga
     3541 tggtccaggc actgctcagc gaaggcaaga ttctctcctg cggcaacggc ggctcagccg
     3601 gcgacgccca gcacttctcc tccgagctgc tcaaccgctt cgagcgcgag cgcccgagtc
     3661 tgccggccat cgccctgacc accgacagct cgaccatcac ctcgattgcc aacgactaca
     3721 gctacaacga gatcttctcc aagcagatcc gcgccctcgg ccaacccggc gatgtgctgc
     3781 tggcgatttc caccagcggt aactcggcca acgtgatcca ggccatacag gccgcccatg
     3841 accgcgaaat gaccgtggtt gccctcaccg gccg
//
LOCUS       DASRUM010000099         9372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2389222, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000099 DASRUM010000000
VERSION     DASRUM010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9372
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2389222"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VFF22_06755"
     CDS             complement(<1..497)
                     /locus_tag="VFF22_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HZX16546.1"
                     /translation="MSDHNQGLAANPGDWPKALFYVALLFSIFQIITAAFHPVSSQIL
                     RAVHVGFLLMVVFLCFPLRGRGQPWQPMAWLLGLAGMATAFYQWYFEADLIQRSGDLT
                     QADMVIGLMLIVLVFEAARRVMGIALPFICALFLVYGLFGQYLPGDLMHRGYALDQIV
                     NQLAF"
     gene            complement(653..1606)
                     /locus_tag="VFF22_06760"
     CDS             complement(653..1606)
                     /locus_tag="VFF22_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZX16547.1"
                     /translation="MRMTKRFSLFAAAAAFTASTAVLAAPTFINVLTGGTSGVYYPIG
                     VGLSQLYSNGIEGSKTSVQATKASVENLNLLQAGRGELAFALGDSVADAWNGVEDAGF
                     KAPLKKLRAIAGTYPNYIQIVASKESGITTLADLKGKRISVGAPKSGTELNARAIFKA
                     AGLSYEDMGKVEFLPYAESVELIKNRQLDATLQSSGLGMAAIRDLAATLPINFVAIPA
                     EITAKIDNAAYQAASIPAGTYDGQDSDVPTVAITNILVSHDGVSDEVAYQMTKLMFDN
                     LERLGNAHSAAKDIKLEGAAKDLPIPLHPGAERFYKEAGAL"
     gene            complement(2010..3362)
                     /locus_tag="VFF22_06765"
     CDS             complement(2010..3362)
                     /locus_tag="VFF22_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZX16548.1"
                     /translation="MTGHVIVVDDEAAIREAVQQWLELSGFRVHSCATAQAALALIDR
                     DYPGIIISDVRMPGTDGLQLLDSVLERDRDLPVILITGHGDVPMAVQALRQGAYDFIE
                     KPFTPERLLDSVRRGLDKRRLVCENRQLRQQFALKDHIEAQLLGVSRPMETLRRQILE
                     LAGTSVNILLRGDTGSGKERVARCLHDFSPRAGKPFVALNCAAIPEHLFESELFGHES
                     GAFTGAQGKRIGRIEHADGGTLFLDEVESLPLAQQVKLLRVLQEKTLERLGSNRSIQV
                     DLRVISAVKPDLLDEVKAGRFREDLYYRLNVATLRIPPLRERREDIPLLFEHFANQAA
                     QRHGRETPSLAPSELTRLLGHDWPGNVRELINAAERHALGLSGGVEGDDGLPQQSLAE
                     QMEAFEAQCLHRALQHCQGNIAEVMALLQLPRRTLNEKMQRHGLQRSSYRPAGSADDE
                     "
     gene            complement(3423..5177)
                     /locus_tag="VFF22_06770"
     CDS             complement(3423..5177)
                     /locus_tag="VFF22_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX16549.1"
                     /translation="MSAFLLRYRVALIVLLILLGLLASIWLAGRYAEQRAWQERSQEA
                     RGQLQLYAQSIHTLVERFRSVPEVLALDSDIKSLLRAPTDSLLRSKLNERLEQLNSAA
                     GSNVLYLLNDQGETLVASNWRDWSSFVGNNYAFRPYFQDAVRQSSGRYFAVGVTTGIP
                     GYFLSHAVRDDNGVVLGVLVVKLELEELQREWVSQPGVLLVADSYSVVILSNRPAWRF
                     LALDSLSEQARAELVEVRKYAEQALQPLPSTLRRQIDSDSQWRRVEGPDGPRDYLWQR
                     QALGDEGWTLHLLHEPKTLVDSVRSYRLAAAGVWMTLAFLLLYLAQRRKTQRLQAGIR
                     ERLEREVALRTAELREAQEGLVHAAKMAALGQMSAAMAHEINQPLTAMQMQLGSLRLL
                     LDSGRQDDVREGLQRIDALLQRIAGLTGHLKTFARKSPAGLSERLRLSDVLEQALQLL
                     APRLRSEQVQLHSEIDSSALVLGDAIRLEQVLLNLLNNALDAMAPVESRQLKIRIERQ
                     AEQCLLSIADSGGGIAAEALGHVFEPFFTTKPVGAGLGLGLAVSYGIVRELGGSLEAA
                     NCEQGAVFTLRLPAAPNA"
     gene            5321..6148
                     /locus_tag="VFF22_06775"
     CDS             5321..6148
                     /locus_tag="VFF22_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003207444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZX16550.1"
                     /translation="MSSTQQTTTPRFWRDPALPFVETREALDGRQLCYAKHSHEYFSI
                     GTITRGRSTYLNEKAREQIAAGTLVLMNPGDVHACNPVDGQLWSYRMFYIDSAWLSDL
                     QHQLGFSHNQPLQPFSAILSRDPALFNGLNQVYDLLNAASVDQLRKECALIDYFSTLQ
                     QRLAPAPTPAREDNPRIRLAAEFIRAHCTQTLKLEAICSAAELSPSYLIRSFRQHYGM
                     TPHAYLLNCRVQVAQAGLKRGESIAEVALDAGFADQAHLQRTFKQLLAATPGQYRRA"
     gene            complement(6149..6736)
                     /locus_tag="VFF22_06780"
     CDS             complement(6149..6736)
                     /locus_tag="VFF22_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HZX16551.1"
                     /translation="MNTLFYSMAGFALAASISPGPVNLLALSAGATHGLRRSMRHVTG
                     ATFGFTALLLAMGLGLQQLLQQWPALAHGVAWFGVAFLLYMAYGLARSDGQLSGNDAQ
                     QRPSLFKGALMQWLNPKAWLASMAGMGAYAADGDLWRVGQFAALYFVICYLSISCWAL
                     AGVYLRQYLENSLYLRRFNRSLALLLVLSAVYLLV"
     gene            complement(6829..7623)
                     /locus_tag="VFF22_06785"
     CDS             complement(6829..7623)
                     /locus_tag="VFF22_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015637555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HZX16552.1"
                     /translation="MRLSDWQHEVQAYLLNSDAQPNPALQASLLGSAALSAEQGLAIY
                     HNAYRARLLEALRGDYPAVHGWLGDEEFDALALAYLDAHPSQHFSLRWLGAQLADFIY
                     GYLVPAQAAPLSELARLEWAFTLAFDAPDGQPLSLVQMAELPAEDWPTLQVRLLPSVQ
                     WLACRHNSLAIWRACKEQGDFPGSQPLVETETCVVWRDQLITRYRSLAVDEATALQGM
                     AVQGWSFAELCGELAELGEQAPVQAVSWLRQWLADGLLQRADHLSR"
     gene            complement(7614..8459)
                     /locus_tag="VFF22_06790"
     CDS             complement(7614..8459)
                     /locus_tag="VFF22_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HZX16553.1"
                     /translation="MNAQRGNLGFGLGLRSTYYQAILEQQPAIDWFEIVSENYLVAGG
                     KALYFLDAIGEQYPLVMHGVSLSIGGPHELDRDYLRQLKHLAARVQPAWISDHLCWSR
                     GNAHQLHDLLPLPYTEESLQHVAERVRQVQDVIERPLVLENVSSYLRCADDQFSEWQF
                     LAALSELSGCELLLDVNNVYVSARNHGFDAWEFISSLPKQRIRQLHLAGHSNYGSYLI
                     DTHDQPVSDPVWQLYQRTLQHLDPVSTLLERDDHFPDLDVLLGELNKARRFAAEALQG
                     TEQCA"
     gene            complement(8516..8773)
                     /locus_tag="VFF22_06795"
     CDS             complement(8516..8773)
                     /locus_tag="VFF22_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16554.1"
                     /translation="MTMKTTAATGAAIAFAAAAMFAGMSGTVVAAEEAKVHCYGVTSC
                     KGQNDCKTAENGCKGQAACKGHGFKAMTQAECDAAGGTVGE"
     gene            complement(8894..>9372)
                     /locus_tag="VFF22_06800"
     CDS             complement(8894..>9372)
                     /locus_tag="VFF22_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HZX16555.1"
                     /translation="LRSELCQQANPEQLFVASAIGQTEGLPVETACALIERPELHLPC
                     VGVAELPERHAWSQIPPHAQRLLLCNGPRCTRRGALDLWKTLRQRLKAAGKLECEGGV
                     HISRTQCQFPCDLGPTASLYPAGEWFRVRDEAELIRLVDERLVAGRAVPELRIDQG"
BASE COUNT         1832 a         3063 c         2889 g         1588 t
ORIGIN      
        1 cagaaggcca gctggttgac gatctggtcc agggcatagc cgcggtgcat cagatcgccg
       61 ggcaggtact ggccgaacag gccgtagacc aggaacagcg cgcagataaa gggcagggcg
      121 atgcccatca cccgccgcgc ggcctcgaat accagcacga tcagcatcag gccaatcacc
      181 atatcggcct gggtcagatc accggagcgc tggatcagat cggcttcgaa ataccactgg
      241 tagaacgccg tggccatgcc ggccaggccg agcagccagg ccataggctg ccagggctga
      301 ccgcggccgc gcagcgggaa gcagagaaac accaccatca gcagaaagcc gacgtgcacg
      361 gcccgcagga tctggctgga caccgggtga aaggccgcgg tgatgatctg gaaaatggaa
      421 aacagcagcg cgacatagaa cagcgctttc ggccagtcgc caggattggc ggcgaggcct
      481 tggttgtggt cactcatgaa ggtcacaccc tcgttacaac gtcgatagcg caagagtcgc
      541 caacaaaacc tgccgtgctc tgcacgccgg ttttattcgc agctctagct cgtttccgga
      601 gaggttggcg ctgcgcacag cgccaaccta cacctgcaac ccttcagcag ccttacagtg
      661 cgccggcttc cttgtagaag cgctcggcac ccggatgcag cggaatcggc agatccttgg
      721 ccgcgccttc cagtttgatg tccttggccg ccgagtgggc gttacccagg cgctcaaggt
      781 tgtcgaacat cagcttggtc atctgatagg ccacttcatc ggatacgccg tcatggctga
      841 ccaggatgtt ggtgatggcc acggttggca cgtcgctgtc ctgaccgtcg taggtgccgg
      901 ctggaatcga cgccgcctgg taagcggcgt tgtcgatttt cgcggtgatc tcggccggga
      961 tagccacgaa gttgatcggc agggtcgcgg ccaggtcgcg aatcgcggcc atgcccaggc
     1021 ccgaggactg cagggtggca tccagctgac gattcttgat cagctcgacc gactcggcgt
     1081 agggcagaaa ctcaacctta cccatgtctt catagctcag gccagcggcc ttgaagatgg
     1141 cgcgggcgtt cagctcggtg ccggatttcg gcgcgccgac ggagatgcgc ttgcccttca
     1201 ggtcagccag agtggtgatg ccggactcct tgctggcaac gatctggatg tagttcgggt
     1261 aggtgccggc aatcgcccgc agctttttca gtggcgcctt gaagccggcg tcttccacgc
     1321 cattccaggc atcggcaacc gagtcaccaa gggcgaaagc cagttcgccg cgaccggctt
     1381 gcagcaggtt gaggttctcc accgaggcct tggtggcctg caccgaggtc ttcgaacctt
     1441 cgatgccgtt gctgtacagc tgcgacaggc ctacgccaat cgggtagtac acaccgctgg
     1501 tgccaccggt cagcacgttg ataaaggttg gagcggcgag taccgcggtg ctggcggtaa
     1561 aggctgcggc agcagcaaac aggctgaaac gtttggtcat tcgcatggaa gaatctccgt
     1621 cgttgttatg gctttatgca gaggttttca ctgtagacac tgcgcgccgc ccaggcagcg
     1681 gtgcagcgcc gggactaacg caatggcgaa aaggagcaga ggctcacgcg cggcctgagc
     1741 cgagcgcaca agaggtgggc agtggatgcc gagcgcgaca ggcgcgcaga cgatgcgggg
     1801 cagtacatgg gattcacctc ttgtctttct tattgaggcc gaggcaatcg agtgcccggc
     1861 tgacaagagc tatagcagat gccgtgccag tgagagaaaa gcccgttcta gagcctttac
     1921 tccaatcagc actgtcagcc tggcggctgt agtaagcgga aatccgccag tgaaaaaact
     1981 cagacaagcg aaattccgcc gactagttct cactcgtcat cagcactgcc ggccggccga
     2041 tagctgctgc gctgcaagcc gtggcgctgc attttctcgt taagggtgcg gcgtggcagc
     2101 tgcagcagcg ccatcacctc ggcgatgttg ccctggcagt gctgcagggc gcggtgcagg
     2161 cattgcgcct cgaaggcctc catctgctcg gccagcgact gttgcggcag gccatcgtcg
     2221 ccctccacac cgccactcaa gcccagggca tggcgttcgg cggcgttgat cagctcgcgc
     2281 acgttgcccg gccagtcatg ccccagcagc cgggtcagct cgctcggtgc caggcttggc
     2341 gtctcgcggc catggcgttg cgcagcctgg ttggcgaagt gttcgaacag caacggaatg
     2401 tcctcacggc gttcgcgcag cggcggaata cgcagggtgg cgacattcag ccggtagtag
     2461 aggtcttcac ggaaacgccc agccttgacc tcatcgagca ggtccggctt gaccgcgctg
     2521 atcacccgca gatcgacctg aatgctgcgg ttggaaccga ggcgctcaag ggttttctcc
     2581 tgcagcacgc gcagcagttt gacctgctgg gccagcggca ggctttccac ctcatcgaga
     2641 aacagtgtgc cgccgtcggc atgttcgatg cggccgatgc gcttgccctg ggcgccggta
     2701 aaggcgccgc tttcatggcc gaacagttcg ctctcgaaca ggtgctcggg aatcgccgcg
     2761 cagttcagcg caacaaacgg cttgccggcg cgcgggctga aatcatgcag gcaccgcgca
     2821 acgcgctcct tgccgctgcc ggtgtcaccg cgcaggagga tatttaccga ggtaccggcc
     2881 agttcgagga tctgccggcg cagggtttcc atcggccgcg agacgccgag caactgcgcc
     2941 tcgatatggt ccttgagggc gaactgctga cgcagctggc ggttctcaca gaccagacgg
     3001 cgcttgtcca ggccgcggcg cacgctgtcg agcaggcgtt cgggggtaaa gggcttttcg
     3061 ataaagtcat acgcgccctg gcgtagcgcc tgcaccgcca tcggcacatc accatgcccg
     3121 gtaatcagaa tcaccggcag gtcgcggtcg cgctccagca cgctgtcgag cagctgcagg
     3181 ccatcagtgc cgggcatgcg cacatcgctg atgatgatgc cggggtagtc acggtcgatc
     3241 agcgccagcg cggcctgcgc ggtggcgcag ctgtgcacgc gaaaaccgga cagctccagc
     3301 cactgctgca ccgcctcgcg gattgccgct tcatcgtcga cgactatcac gtgcccagtc
     3361 atatcacctc ctgatcaatc ggcggcagtg tagcccggat aaaatccagg aaataacccg
     3421 cactacgcat tcggcgctgc cggcaagcgc agggtgaaca ccgcgccctg ctcgcaattg
     3481 gccgcctcca ggctgccgcc cagttcgcgg acgatgccgt aggacacagc cagccccagg
     3541 cccaatccgg cgccaaccgg cttggtggta aagaacggct cgaacacatg acccagtgct
     3601 tccgcagcga ttccgccgcc actgtcggcg atgctcagca ggcactgctc agcctgacgc
     3661 tcgatacgga tcttcaattg ccgcgactcg acaggcgcca tggcgtccag ggcgttgttc
     3721 agcagattga gcagcacctg ctccaggcga atcgcatcac ccagcaccag cgcgctgctg
     3781 tcgatctcac tgtgcagctg tacctgttcg ctgcgcaggc gcggcgccag cagctgcagg
     3841 gcttgctcca gcacatcgct cagacgcaga cgctcactca gaccggccgg gctttttcgg
     3901 gcaaaggttt tcaggtggcc agtgagcccg gcgatgcgtt gcagtaaggc gtcgatacgt
     3961 tgcaaaccct cacgcacatc gtcctggcgg ccgctgtcga ggagcaggcg caggctaccg
     4021 agctgcatct gcatggcagt cagcggctga ttgatctcat gggccatggc cgccgacatc
     4081 tgccccagcg cggccatctt cgccgcatgc accaggcctt cctgggcctc gcgcaattcg
     4141 gcggtacgca gcgccacttc acgctccagg cgctcacgaa tgccggcctg caggcgctgc
     4201 gtcttacgcc gctgggccag gtaaagcagc agaaacgcca gggtcatcca caccccggcg
     4261 gcggccaggc gatagctacg cacgctgtcg accagggttt tcggctcgtg cagcaggtgc
     4321 agggtccagc cttcatcgcc cagggcctgg cgttgccaga ggtagtcacg tgggccgtcc
     4381 ggcccctcaa cccggcgcca ctggctgtcg ctgtcgatct gtcggcgcaa cgtgctgggc
     4441 agtggttgca gcgcctgttc ggcgtacttg cgcacctcca ccagctcggc acgggcctgc
     4501 tcgctcaagc tgtcgagggc gagaaaacgc caggccgggc ggttgctgag gatcaccacc
     4561 gagtagctgt cagccaccag cagcacgccg ggctggctga cccattcgcg ctgcaactcc
     4621 tccaactcca gcttgaccac cagcacgccg agcaccacac cgttgtcgtc acgcaccgca
     4681 tgggagagga aataaccggg aatgccggtg gtcaccccca ccgcaaaata acgcccggat
     4741 gactgccgta cagcatcctg aaaatacggg cgaaaggcgt agttgttgcc gacaaaactg
     4801 ctccagtcgc gccagttgct ggccaccagc gtttcgcctt gatcgttgag caggtacagc
     4861 acgttggaac cggcagcgct gttgagttgc tccaggcgtt cattgagttt gctgcgcagc
     4921 aggctgtccg ttggcgcgcg cagcagcgac ttgatatcgc tgtccagcgc cagcacctcg
     4981 ggcactgaac gaaagcgctc gaccagggtg tggatcgact gcgcatacag ctgcaactgc
     5041 ccgcgcgctt cctgactgcg ctcctgccag gcgcgctgct cggcatagcg cccggccagc
     5101 cagatactcg ccagcaagcc gagcagaatc agcaggacga ttagggcaac gcggtaacgc
     5161 agcagaaagg cagacatgcg cggctcgcaa ggtgggggag ccggcatggt aagacaaagc
     5221 accgcgccgt gcagagcgta gccgctcgac tgtttgaaca agcgcccccg gccgctatag
     5281 tctcggcttg gctacgcggc gatggcgcca gggaaacaag atgagcagta cacaacagac
     5341 taccacccca cgcttctggc gcgacccggc gctgcccttc gtggaaacgc gcgaagccct
     5401 cgatggccgc cagctgtgct acgccaagca ttcccatgag tacttttcga tcggcaccat
     5461 cacccggggg cgcagcacct acctcaatga aaaggctcgc gagcagatcg ccgccggcac
     5521 gctggtgctg atgaacccgg gtgacgtgca tgcctgcaac cccgtggatg gacagctctg
     5581 gtcgtatcgc atgttctata tcgacagcgc ctggctgagc gacctgcaac accagttggg
     5641 ctttagccac aaccagccac tgcagccctt ctccgccatc ctcagccgcg accctgcgct
     5701 gttcaacggc ctcaaccagg tgtacgacct gctcaacgcc gcatcggtcg atcagctgcg
     5761 caaagagtgc gccctgatcg actatttcag caccctgcaa cagcgcctgg cacccgcacc
     5821 gacacccgca cgcgaggaca atccgcgcat ccgcctggcg gctgaattta tccgcgcgca
     5881 ctgcacccag acgctcaagc tggaagccat ctgcagcgcc gccgagctgt cgccctcgta
     5941 cctgatccgc agtttccgcc agcactacgg catgaccccg catgcctacc tgctcaactg
     6001 ccgcgtgcaa gtggcccagg ccgggctcaa acgcggcgag tcgattgccg aggtggcgct
     6061 ggacgccggc tttgccgacc aggcgcactt gcagcgcacc ttcaaacagc tgctcgccgc
     6121 tacccccggg caataccgcc gcgcctgatc agaccagcag ataaaccgcc gacagcacca
     6181 gcagcaaggc caggctgcga ttgaaccggc gcaggtacag cgaattctcc aggtactggc
     6241 gcaggtaaac ccccgccagc gcccaacagc tgatcgacag gtagcagatg acgaaataca
     6301 gcgccgcgaa ctgccccacc cgccacagat cgccatccgc cgcataggcg cccatgcctg
     6361 ccatcgaggc cagccaggcc ttggggttga gccactgcat cagcgcgccc ttgaacagcg
     6421 acgggcgctg ctgtgcgtcg ttgccactga gctgcccgtc agaccgggcc aggccatagg
     6481 ccatgtacag aagaaaggcg acgccaaacc aggccacccc gtgcgccagc gccggccact
     6541 gctgcaacag ctgctgcaag cccagaccca tggccagcaa cagcgcggta aaaccgaacg
     6601 tcgcccccgt gacatgacgc atgctgcggc gcagcccatg ggtcgcaccg gcactgaggg
     6661 ccagcagatt gaccgggccg ggagaaattg aggcagcgag ggcaaaaccc gccatggagt
     6721 aaaacagcgt attcatcaca cacctgtcag cacaaccaaa ggaggtgcca ggctgacagg
     6781 cccacggcgc agggtattga aggaaaatgc cctgtcaggg caggacaatc agcgcgagag
     6841 gtgatccgcc cgctgtaaca gcccgtccgc cagccactga cgcaaccagc tcactgcttg
     6901 caccggcgcc tgctcgccca gctcagccag ctcgccgcac agctcggcga agctccagcc
     6961 ctgcacagcc atgccctgca acgccgtggc ttcatcgacc gccaggctgc ggtagcgggt
     7021 aatcagctga tcgcgccaga ccacacaggt ctctgtctca accagtggct ggctgccggg
     7081 gaagtcaccc tgctccttgc acgcgcgcca gatcgccagg ctgttatgcc gacacgccag
     7141 ccactgcacg ctcggtagca aacgcacctg cagggtcggc cagtcttcgg ccggcagttc
     7201 ggccatctgc accaggctca ggggctggcc atctggcgca tcgaaggcca gggtaaaggc
     7261 ccattccagc cgcgccagtt cgctgagcgg agcggcctgg gccggcacca gatacccgta
     7321 gataaagtca gccagctgcg cgccgagcca gcgcaggctg aaatgctgcg aagggtgcgc
     7381 gtcgagatag gccagggcta gggcatcgaa ttcctcatca ccgagccagc catgcaccgc
     7441 cgggtaatca ccacgcaagg cttccagcag gcgcgcgcgg taggcgttgt ggtaaatcgc
     7501 caggccctgt tcggcactca gcgccgcact gcccagcagg ctcgcctgga gcgctgggtt
     7561 cggctgggca tcagagttca gcaggtaggc ctgcacctcg tgctgccagt cgctcaggcg
     7621 cattgctcag ttccttgcag cgcttcggcg gcaaaccggc gcgctttgtt cagctcaccg
     7681 agcagcacgt ccagatcagg gaaatggtca tcgcgctcaa gcaaggtgga caccgggtca
     7741 aggtgctgca aggtgcgttg gtagagctgc cagaccggat cgctgaccgg ttgatcgtgg
     7801 gtgtcgatca ggtagctgcc gtaattacta tggcccgcca agtgcagctg acgaatacgc
     7861 tgcttgggca ggctgctgat aaattcccag gcatcgaaac catggttgcg cgcgctgacg
     7921 tagacattgt tgacgtccaa caacagctcg cagccgctca actcgctgag tgcggcgagg
     7981 aactgccatt cgctgaactg atcatcggcg cagcgcaggt aactggagac gttttccagc
     8041 accagcggcc gctcgatcac gtcctgcacc tggcgcacac gctcggccac atgctgcagg
     8101 ctctcctcgg tataaggcag cggcagcagg tcgtgcagtt gatgggcatt gccccggctc
     8161 cagcacaggt gatcggagat ccacgctggc tgcacgcgtg cggcgaggtg tttgagctgg
     8221 cgcaggtagt cgcggtccag ctcatgcggg ccgccaatcg acagcgacac gccatgcatc
     8281 accagcgggt actgctcgcc gatggcatcg agaaagtaga gggccttgcc gcccgctacc
     8341 aggtaatttt ccgagacgat ctcgaaccag tcgatcgccg gctgttgttc gagaatggct
     8401 tggtaatagg tgctgcgcag gccgaggcca aagcccagat tgccgcgttg agcattcatt
     8461 gctggctcct tgcagggtga tgcggggcaa ccgcgcggcg gccgccccgg ctcacttact
     8521 cgccgacagt accgcctgca gcatcgcact cagcctgggt catggccttg aagccgtggc
     8581 ccttgcatgc agcctggcct ttgcagccgt tctcagcggt cttgcagtca ttctgacctt
     8641 tgcaggatgt aacgccgtag caatgcacct tggcctcctc tgccgccaca acagtgccgg
     8701 acataccggc aaacatggca gcagcagcga aagcgatggc ggcacccgtt gcagcagtag
     8761 ttttcatagt catgtgcata tcctcaggtg atcttgattg gccgggcgaa aggttttttg
     8821 cgtgccggca ttcaactaga gcgacgaggc gcgctggtgt tacacagccc atgaaaaaaa
     8881 cagccgcgcg cactcagccc tgatcgatac gcagctcagg cactgcacgg ccagccacca
     8941 gacgctcatc gaccaggcga atcagctcgg cttcatcacg cacgcggaac cactcaccgg
     9001 ctggatagag gctggccgtc gggcccaggt cgcagggaaa ctggcactgc gtacggctga
     9061 tatgcacccc gccctcgcac tccagtttgc cggcggcctt gaggcgttga cgcagggttt
     9121 tccacaggtc caacgcgcca cgccgggtac agcgcgggcc gttacacagc agcagacgct
     9181 gggcgtgcgg cggaatctgc gaccaggcat ggcgctccgg cagctcagcc acgccgacgc
     9241 agggcaggtg cagctctggg cgctcgatca gcgcgcaggc ggtctccacc ggcaagcctt
     9301 cggtctggcc aatggcgcta gcgacaaaca gctgctccgg gttggcctgc tggcatagct
     9361 cgctgcgcag cc
//
LOCUS       DASRUM010000100        13593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1723202, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000100 DASRUM010000000
VERSION     DASRUM010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 13593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13593
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723202"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1511
                     /locus_tag="VFF22_06805"
     CDS             282..1511
                     /locus_tag="VFF22_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX16556.1"
                     /translation="MHYRTDYCAAQLNEAPQVLHLGDLRLHYQAYACRSNDPRPPVLL
                     LGGAFQSFRSFAAEVSELLVDHPVILLDLPSQGGNLQLVPDLNLEQLADLIAAFAGEL
                     DLPPLMPIGLSYGSALAALFAARHPERCARVLLAGITAFGRPGARLLLQEGLALLAEG
                     RCEAFAHGALTGLINPLRLEQTGVTPGFRKALLRQIQRLSPTDIERYRQNSQRLLDFQ
                     GFRTHPSCPTLVLAGEYDHFTQPWEHAHFAAACTQASCALIHNADHLAQFEQRNACAN
                     LYNPFLRGESLPSRSPGSSLMPQRQLLQLEKRHEPRLPPNQRQARLQHAEGGEWTVDI
                     PELGFFGGLLQGDLPAICGMRGWQLLCKDLPAQPLLPLRQDQNGLAFVFPHSDQQASQ
                     ALATQFQLQPLPAAACA"
     gene            complement(1615..2469)
                     /locus_tag="VFF22_06810"
     CDS             complement(1615..2469)
                     /locus_tag="VFF22_06810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX16557.1"
                     /translation="MSERARVSSAHISPSAHYTGYVWYRHQLADAAFATGFGRFVHGL
                     LSPITWGARVGFGLDIEQFLLQRHLQIDAQLTAAIERDGVCQVVEIACGLSPRGRRFT
                     ARYPQLRYIEADLPVMASRKRLLLNSEGWLGSQHQVRAVDILAEDGSKSLTELFAELD
                     HSKPVVVITEGLVNYFELALIESFWARLAEQLRQFPQATYLTELYPDLREHPRYRQLR
                     WCVGLIGRLTRGNYPLHYRNDQAIVQGFEKCGFRQVTVFDPSRTSQALGLAPSRSAGL
                     VRVIEARM"
     gene            2631..3947
                     /locus_tag="VFF22_06815"
     CDS             2631..3947
                     /locus_tag="VFF22_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DedA family/phosphatase PAP2 family
                     protein"
                     /protein_id="HZX16558.1"
                     /translation="MSQWFDTLTLWLSANPQWLGLAIFLLACAECLAIVGILVPGTVL
                     LFAVAALAGNGALGLGETLLLAFCGGLLGDCLSYALGRYFHQDIGRLPVLRHHPQWLN
                     SAHGYFVRYGVASLLVGRFIGPLRPMLPMVAGMFDMPFARFFLVSLLAGIGWSIAYML
                     PGWATGAAMRLPLPEGFWTQAGAIAAGVALVVGISVQGSLRGQRHTSLLTAAASLALL
                     VGLMLSWPHLGPLDQGLLTVAQEHRSPSLDNLMVLITRLGDMHTQLAAAVLLCLILLA
                     TRQWRTLCLAIGTLLGTALANGALKAFFARNRPDVLLEPLGSFSFPSGHSSAAFAFCL
                     LLGVLAGRGQPTRMRLTWLLLASLPAAAIAGSRIYLGVHWPSDIIAGAILAACFCALS
                     LALVQWRTPLPAMSARIWWLLLPAVMALLGGIATWQFSAGQLLYRY"
     gene            complement(3941..4528)
                     /locus_tag="VFF22_06820"
     CDS             complement(3941..4528)
                     /locus_tag="VFF22_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HZX16559.1"
                     /translation="MTLPLFPLNTVLFPGCMLDLKIFEARYLDMISRCMKQSQGFGVV
                     CIIDGAEVGEAAGQFSAIGCEAQIRDFQQLPNGLLGIRVEGGRRFRVQRAQVLPDQLT
                     LAEVEWLDEQPEQPLEAEHADLVALLGALSAHPMVASLGMASEPAGQEALANQLAYLL
                     PLDAEQKLQLLETPEVQLRLELLQQLLELLQGERQ"
     gene            complement(4539..5249)
                     /locus_tag="VFF22_06825"
     CDS             complement(4539..5249)
                     /locus_tag="VFF22_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020305837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HZX16560.1"
                     /translation="MEWHAAWQALIHHPLFGVGITLGAYQLAIAAYEKTRWVFLQPVL
                     VSMLTVIGILLACGLTFAEYKDSAAALTLFLGPTTVALAVPLFLNLRRIRQLFWPTLI
                     TLLVAGVVATVLGITLAWAFGAEQIMLMSMAPKSVTSPIAMLVANQIGGIAALAAVFV
                     MITGIIGAIIGPALLKRCGVQHPAALGMALGLTAHAVGTARALQEGEECGAFSALAMS
                     LMGVFTAVLLPLAILLWL"
     gene            complement(5252..5611)
                     /locus_tag="VFF22_06830"
     CDS             complement(5252..5611)
                     /locus_tag="VFF22_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HZX16561.1"
                     /translation="MLLRGLSWLVLCQLLGTALNVLLIPILPGPIIGMLLLFGFLLLR
                     GEVGEPLHLASTSLLKYLPLLLVPPAVGVMAYAEDIFADFWAIAGALLLSLLISIVFA
                     GWLMQKLIDRQQRREDA"
     gene            5829..6284
                     /locus_tag="VFF22_06835"
     CDS             5829..6284
                     /locus_tag="VFF22_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003176308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZX16562.1"
                     /translation="MTSVPAAELPSYIGKELGRSDWLTIDQERINQFAECTGDHQFIH
                     VDPEKAKLTPFGSTIAHGFLSLSLVPKLMEGIMIMPKGLKMAVNYGLDSVRFIQPVKV
                     NSKVRLVVSLTDAHEKNPGQWLLKAKVVLEIEGSEKPAYIAEPLTLCFV"
     gene            6426..8153
                     /locus_tag="VFF22_06840"
     CDS             6426..8153
                     /locus_tag="VFF22_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016986106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HZX16563.1"
                     /translation="MRPLSLLAPLSLALLLIACGDGEPLLPPDAVLPDGGRYRGEVID
                     GLLQGPGRLDYSDGSYYQGQFKDGQFNGQGEWRSKHGEFYSGEFQGGDFHGQGSLTYS
                     DGSRYEGGFVNGRPSGEGHLKGSQMEYRGEFSKGQYSGLGKLQWSNGASFQGQFKKGE
                     PDGQGVLTDAEGNEYTGTFDNSQLNGQGSFKNADGDLYSGQFRDNQFHGKGRYQSAGG
                     EVWSGRFVHGSLTGKGEFKGSDGSHYVGQFSDWTYHGEGRLNLVDGSLYQGNFAYGQY
                     QGIGSLTLVDGSQQSGTWQNGRLTRDAQGKAIPDALATGLLEQGRLLDAAIAKLPAST
                     PAIELYALTLAGDGKQSVFMREADYVGNLLQERFAAHGLITLVNHRDHLADRPLATSA
                     SLDRSVQALAERSGKEDLIFIYLTSHGSAEHELNLDQPRLQLNDLPASDLAALLEPLQ
                     DRHKVLVISACYSGGFIPKLQDDKTLVITAARADRVSFGCSEENDFTYFGRALFAEAL
                     QQTDDLQRAFELAQTSVAAREKADGFEPSEPQIWPAKAVLAQWRKLREQQAERALNNA
                     LEVQSAVNP"
     gene            8215..8856
                     /locus_tag="VFF22_06845"
     CDS             8215..8856
                     /locus_tag="VFF22_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HZX16564.1"
                     /translation="MYLTPQHILLAGATGLTGEHLLDRLLNEPTVARVLAPSRRPLAA
                     HAHLENPQGELLALLPQLSGKVDTAFCCLGSTIKQAGSQEAFRAVDHDLVIAFAERAR
                     ALGARHLLVISALGADANSTVFYNKVKGEMEQALRAQGWPQLTIVRPSLLLGPRQEFR
                     LGERLAAPFMRWLPGKYRGIEATVLARALWRLALEEDAGTRVIESDQLRRLVR"
     gene            8885..9235
                     /locus_tag="VFF22_06850"
     CDS             8885..9235
                     /locus_tag="VFF22_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5629 family protein"
                     /protein_id="HZX16565.1"
                     /translation="MSSTPHDLISALSGADMLEIDELHAWQFSLNAEQLAQHQAGSPP
                     TGDAPLLSIECMDGRALRKWHFSLAQVIAARFDGEADAWRISGAAYTHLIKCFAAVSG
                     DNSDMPDDASNNAE"
     gene            9255..9866
                     /locus_tag="VFF22_06855"
     CDS             9255..9866
                     /locus_tag="VFF22_06855"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016254109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX16566.1"
                     /translation="MGLYDRYLLPHLIDFACGMGAVMKARSQLVPQAHGRVLEIGIGS
                     GLNLSFYDASKVSVIIGVDPSADMQKLAKQRAAAISIPVEMIALELGQIRAEDASFDC
                     IVCTFTLCTIPDAVTALKEMRRVLKPGGRLLFCEHGLAPELPVLRWQNRLTPLWKPLA
                     GGCHLNRDIKALITAGGFQIGELSNRYLKGPRPMTYIYQGWAQ"
     gene            9962..12541
                     /locus_tag="VFF22_06860"
     CDS             9962..12541
                     /locus_tag="VFF22_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015096626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZX16567.1"
                     /translation="MKRLSFHHLTTLLLFIASLTAVSLSGAQSRQVSVGVYANEPKIL
                     LGQDGKLSGILGELLNEIAQQEDWQLRPRPCEWQECLELAQSGEIDLMPDVAYNDSRA
                     QVLDFHKVPSLVSWSQVYSSENLQLRSILDLKGLRIAVLQGSVQEEYLRGLLTSFGLK
                     AQLIGVPSLPQGFEQVAAGQADAVVANQRFGDFHAPSYQLQSSSIMFQPAHLFYATRK
                     GQNADLLAAIDQHLANWQDSPSSIYYQSLQRWGGERPRMLIPGSVWWGLSTLALLLIA
                     ALGGALLLRRQVGEKTRHLLASEERLNTILDSVEAYIYIKDAQLRYQYANRKVCELFG
                     QKLEQVIGKTDEQFFDIDTATNLHDNDRRVLKLGERVQTEEINRSLDGSTQHAFLSVK
                     LPLRDGQGNIYALCGISTDITEHKKNLEKINQLAFYDPLTGLPNRRLLLDRLQQALAK
                     HARGLQQGALLFIDLDNFKDLNDTLGHDMGDQLLQQVAQRLSSHVRAQDTLARLGGDE
                     FVLMLEGLSLKPAQAGQQVESVSRKIIEALAAPYALQSRSHTSTASIGIALFPHERST
                     VDEVLKRADMAMYQAKAAGRNAMRFFDPTMQADINARASLESDLRQSLGQRDFILHYQ
                     PQVDAQGQLVGAEALVRWQHPTRGLVPPGEFIPLAESTGLILPLGRLILHSACQQLVA
                     WAAHPQLAQLTLAVNVSARQFHHPDFVEDVFATLAESGANPQRLELELTESQLVEDVE
                     VLIGKMSQLKARGIHLALDDFGAGYSSLNYLKRLPLDQLKIDQSFVRDLLEHPSDAAI
                     VRTILALGANLNLAVTAEGVETQDQYEALQRMGCRRFQGYLFAKPGPASALEQWHNLT
                     PTE"
     gene            complement(12548..13183)
                     /locus_tag="VFF22_06865"
     CDS             complement(12548..13183)
                     /locus_tag="VFF22_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZX16568.1"
                     /translation="MQQRVYVVDDDQGMLDSTVWLLESVGLTALPFTSGRAFLDACDE
                     RSQGCVLLDVRMPGMGGLNVQEAMRSRGIQMPIIFVSGHADVPIVVRAFRAGAVDFIE
                     KPYNEQLLLDSVQQALSHAPAAAQADPRLQAVEQRLASLTPRERDVLLPLVRGYTNRE
                     MAEQLDISVKTIDLYRSRVMKRMQADTLADLVGMAIAAGLLDALQLRGEQP"
     gene            complement(13226..>13593)
                     /locus_tag="VFF22_06870"
     CDS             complement(13226..>13593)
                     /locus_tag="VFF22_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX16569.1"
                     /translation="EQVLLNLLRNAIDANREAHPGQASRIELSAAEQDGQLCVRVWDQ
                     GPGVAQGELEHIFTPFYTSKAEGLGLGLSMSRSIIEGFGGALEAQPGEGGGLRLECRL
                     PLLRGEDAESLDGTVTGRG"
BASE COUNT         2746 a         4589 c         3906 g         2352 t
ORIGIN      
        1 agcacggact cagtcatggt cttggcaggt ctggcggttg aaaaaagtcg ctgagtataa
       61 cggtcgcgcc gctccacgcc cagcgctgcg cggcggacgg tagacacggc gttcgcggca
      121 gcctgcggta acgccgccgg accacgtact gcaggcctcc cggtgaaaca gtgacaccag
      181 caccacgctg ccgcccgaag gttaggtaaa caaaaaccgc tcgaatgttt accttgccat
      241 taaaagcaag acaggcggcc gaccgccggg gtgggattaa catgcattac cgtaccgact
      301 actgcgccgc acaactcaac gaagccccac aggtgcttca cctcggtgat ctgcgcctgc
      361 actatcaggc ctacgcctgc cgcagcaatg acccgcgacc acccgtgctg ctgctcggtg
      421 gcgccttcca gagcttccgc tcgtttgccg ccgaggtcag cgagctgctc gtcgaccacc
      481 cggtgattct gctcgacctg cccagccagg gtggcaacct gcaactggtg cccgacctga
      541 acctggagca gctggccgac ctgatcgccg cattcgccgg cgaacttgac ctgccgccgc
      601 tgatgcccat tggcctgtcc tacggctctg ccctggctgc gctgtttgcc gcccgccatc
      661 cggagcgctg cgcccgcgtg ctgttggccg gaatcaccgc ttttggccgc cccggcgcgc
      721 gcttactgct gcaggaaggc ctggcactac tggccgaggg ccgttgcgag gcctttgccc
      781 acggtgcgct gactggtctg atcaatccct tacgcctgga acaaaccgga gtgacccctg
      841 gatttcgcaa agcactgctg cggcagatcc agcgcctgtc gcccaccgat atcgagcgct
      901 accggcagaa cagccaacgc ctgctggatt tccagggttt tcgcacgcat cccagctgcc
      961 cgaccctggt actggccggc gaatacgatc acttcaccca gccctgggag cacgcgcact
     1021 ttgccgctgc ctgcacccag gccagttgtg ccctgatcca caacgccgat cacctggcgc
     1081 aattcgaaca acgcaacgcc tgcgccaacc tgtacaaccc atttctgcgc ggcgaatccc
     1141 tgccaagccg cagcccaggc agcagcctga tgccccaacg gcagctgctg cagttggaaa
     1201 aacgtcatga gccacgcttg ccccccaacc agcgccaggc tcgcctgcag catgccgaag
     1261 gcggcgaatg gacagtagat atccccgagc tgggtttttt cggcgggctg ttgcagggcg
     1321 atctgccagc gatttgcggc atgcggggct ggcaactgct gtgtaaggac ttgcccgccc
     1381 aacccctgct accgctgcgt caggatcaaa acggcctggc ctttgtcttc ccacacagcg
     1441 accaacaagc cagccaggcg ctggcgacgc agtttcagtt acaacctttg ccggcagcag
     1501 cctgtgcgta ggctgcgtta gcggcaaggt gaactcaagc cggcccacct tgccggcgcc
     1561 gcgcaaccca acgaacccca gccccgcata caccccacgc tctgactcgg gcgcttacat
     1621 ccgagcttcg atcacccgca ccagccccgc actgcgcgac ggcgccaagc caagcgcctg
     1681 gcttgtgcgg ctcgggtcaa acaccgtgac ctgcctgaaa ccgcacttct caaagccctg
     1741 cacaatcgcc tgatcattgc ggtagtgcag cggatagttg ccacgggtca gtcgaccgat
     1801 caagccgaca caccagcgca gctgacgata acgcgggtgc tcacgtagat ctgggtacag
     1861 ctcggtcagg taggtggcct ggggaaactg gcgcagctgc tccgctaaac gcgcccagaa
     1921 cgactcgatc agcgccagct caaagtaatt caccaatccc tcggtaatca ccaccaccgg
     1981 cttgctgtga tccagctcgg caaacagctc cgttaggctt ttgctgccat cctcggcgag
     2041 gatatccacc gcacgcacct gatgctggct acccagccaa ccctcgctat tgagcagcag
     2101 ccgcttgcgc gacgccatga caggcagatc ggcctcgata taacgcagct gcggataacg
     2161 tgcggtaaag cgccggccac gcggcgacag gccgcaggca atctccacca cctgacacac
     2221 cccatcccgc tcaatcgccg cagtcagctg cgcatcaatc tgcagatgcc gttgcagcag
     2281 aaactgctca atatccaggc caaacccgac ccgcgcgccc caggtgatcg gactgagcaa
     2341 accatgcaca aaacgcccga agccagtagc aaaggccgca tccgccaact ggtgtcgata
     2401 ccagacataa ccggtgtaat gggcactagg gctgatatga gcgctgctga cgcgggcacg
     2461 ttcagacatg gttaagactc ttatttttat gatcgcccca gcctatgccc tgccgagtga
     2521 ccgtgacacc gcatgtcagc cgaacccacc cttagtttgc tctggcgcga agcctgcaca
     2581 ttaccggccc agcagggtta ttaccgatta atcatcatgg atgaccagcg atgagccaat
     2641 ggttcgacac cctgaccctc tggctaagtg ccaaccctca atggctgggc ctggcgattt
     2701 tcttgctggc ctgcgccgag tgcctggcga ttgtcggtat tctcgtcccc ggcaccgtac
     2761 tgctgttcgc cgtcgccgcg ctggccggca atggcgcgtt gggcctgggt gaaaccctgc
     2821 tgctggcgtt ttgcggcggg ttgctgggcg actgcctgtc ctatgcgctg gggcgctatt
     2881 ttcatcagga tatcggccgc ctgccggtgc tgcgccatca tccgcaatgg ctgaacagcg
     2941 cccacggtta cttcgtgcgc tatggcgtcg ccagcctgtt ggtcgggcgc tttattggtc
     3001 cgctgcggcc catgctgccg atggtcgccg gcatgttcga catgcccttc gcgcgttttt
     3061 ttctcgtcag tttgctggcc gggatcggct ggtcgatcgc ttatatgctg cccggctggg
     3121 cgactggcgc cgccatgcgc ctgccgctgc cggaaggttt ctggacccag gccggggcca
     3181 ttgccgccgg tgtggcactg gtagtgggca ttagcgtgca aggcagcctg cgcggacaac
     3241 ggcacaccag cctgctgacg gcggcggcga gcctggccct gctggttggc ctgatgctca
     3301 gctggccgca cctgggcccg cttgatcagg gcctgttaac cgtggcccaa gagcatcgca
     3361 gcccaagcct agacaacctg atggtgctga tcactcgcct cggcgatatg cacacacaac
     3421 tggccgcggc cgtgctgctg tgcctgatcc tgctggcaac ccggcaatgg cgtacgctct
     3481 gcctggcaat tggcaccctg ctcggcaccg cgttggccaa cggtgcgcta aaggcattct
     3541 tcgcccgtaa ccgccccgat gtgctgctgg agccgctggg cagtttcagc tttcccagcg
     3601 ggcacagctc ggcagccttc gccttctgtc tgctgcttgg ggtactggcc ggtcgcgggc
     3661 aaccgacccg tatgcgcctg acctggttgc tgctggccag cctgccggcc gcagcaattg
     3721 ccggctcacg gatttacctg ggggtgcatt ggcccagcga cattatcgct ggcgccatac
     3781 tggccgcctg tttctgcgcc ctgagcctgg ccctggtgca atggcgcacg ccactgccgg
     3841 cgatgtcggc aaggatctgg tggttgctgc tgccggcggt tatggcgctg ctcggcggca
     3901 tcgctacctg gcaattctcc gccgggcaac tgctgtaccg ctactgacgc tcgccctgca
     3961 acagctccag cagctgttgc agcagttcca ggcgtagctg cacctcgggc gtttcgagca
     4021 gctgcagctt ctgctcggcg tccagcggca acaggtaggc cagctgattg gccaatgcct
     4081 cctgacccgc tggttcgctc gccataccca gcgatgccac catcgggtgg gcgcttagcg
     4141 caccgagcaa cgccaccagg tcggcgtgtt cagcctccag cggctgctcc ggctgctcat
     4201 ccagccactc gacctcggcc agggtcagtt gatccggcag cacctgggcg cgctgaacgc
     4261 gaaaacgccg gccaccctca acgcgaatgc ccagcaggcc attgggcagc tgctggaaat
     4321 cgcgaatctg cgcctcgcag cctatcgccg aaaactgccc ggcagcctcg cccacctctg
     4381 caccatcgat gatgcacacc acgccaaagc cctggctctg cttcatgcag cggctgatca
     4441 tgtccaggta gcgcgcttcg aatatcttca ggtcgagcat gcagccgggg aacagcacgg
     4501 tattcagtgg aaatagcggt agggtcacag gcgcctcctt atagccacaa cagaatagcc
     4561 agcggcagca gcacggcggt gaacacgccc atcaggctca tcgccagggc agaaaatgcg
     4621 ccgcactcct caccttcctg cagcgcccgc gcggtgccga ccgcatgcgc ggtcaggccc
     4681 agggccatgc ccaatgcggc cggatgctgc acgccacagc gtttgagcag cgctgggcca
     4741 atgatggcgc cgatgatccc ggtgatcatc acaaacaccg cggccagggc ggcaatcccg
     4801 ccaatctggt tggccaccag catggcaata ggcgaggtca ccgatttcgg cgccatgctc
     4861 atcagcatga tctgctcggc accgaaggcc caggccaggg taatacccag cacggtggca
     4921 accacaccgg ccacaagcag ggtgatcagc gtcggccaga acagctggcg aatccgccgc
     4981 agattaagaa acagcggcac cgccagcgcc accgtggttg gcccgaggaa cagcgtcaac
     5041 gcagcggcgc tgtccttgta ttcggcaaag gtcaggccac aggccaggag aataccgatc
     5101 actgtgagca tcgacaccag caccggctgc agaaacaccc agcgggtttt ctcataggcg
     5161 gcaatcgcca actggtaggc accgagggtg attccgacgc caaacagcgg gtgatgaatc
     5221 agcgcctgcc aggcggcgtg ccattccaga gtcatgcgtc ctcccggcgt tgctgacggt
     5281 cgatcagctt ctgcatcagc cagccggcaa acacgatcga gatcagcagc gacagcaaca
     5341 gcgccccagc aatcgcccag aagtcggcaa agatatcctc ggcataggcc atcaccccca
     5401 ccgctggcgg caccagcagc aacggcaggt atttcagtag gctggtagag gccaggtgca
     5461 gcggctcgcc cacctcgccg cgcagcagca gaaagccaaa cagcagcaac atgccgatga
     5521 tcggccccgg caggatcggg atcagcagga cattcagcgc agtgccgagc aactggcaca
     5581 gcaccagcca ggaaaggccg cgtagcagca tgatgggtaa tctccgaatc gtatgatccc
     5641 agacgcagtg ccgcgcatta taagcagaag cgcccggcaa gggccggttt acagcggttg
     5701 cgtggcaatc ggccaacgtt tacgacgcat ctacgaggca cttgaccctg cggcaaaccc
     5761 atgatgatct tagggcagaa ggattaacgc cccaaaaaaa acaacacttg agtcttcaag
     5821 gagaaaccat gacatcagta cccgccgccg aattgcccag ctatatcggc aaagaactcg
     5881 gccgttccga ctggctgacc atcgaccagg aacgcatcaa ccagttcgcc gaatgcaccg
     5941 gcgatcacca gttcatccac gttgacccag aaaaagccaa actcaccccg tttggcagca
     6001 ccatcgctca cggtttcctc tcgctatcac tggtgcccaa gctgatggaa ggcatcatga
     6061 tcatgcccaa agggctgaag atggccgtca actatggcct ggatagcgtg cgctttatcc
     6121 agccggtgaa ggtcaattcc aaagtccgtc tggtggtcag cctgaccgac gcccacgaga
     6181 agaaccccgg ccaatggctg ctcaaagcca aggtcgtgct ggagattgaa ggctcggaaa
     6241 aacccgccta tatcgccgaa ccgctgaccc tctgcttcgt ctaaacgcag ccgccgaaca
     6301 agggcgcacc ggtatctgcc ggtgcgcctt ttctatttat atatgcctgc cggtgcgctc
     6361 aatcacggca ctccgagcgc tttaaggcat actcggcaac gaccttaaca gcctggattt
     6421 tctgcatgcg ccctctctcc ctcctggccc cactgagcct cgccttgctg ctgatcgcct
     6481 gcggcgatgg cgaacccctg ctgccaccgg atgccgtgct gcctgatggc gggcgttacc
     6541 gtggcgaagt gatcgacggc ctgctgcaag gcccaggccg cctggattac agcgacggca
     6601 gctactacca ggggcaattc aaggacggcc agttcaacgg ccagggcgaa tggcgcagca
     6661 aacacggcga gttctacagc ggcgaatttc agggtggcga cttccacggc cagggcagcc
     6721 tcacctacag cgacggcagc cgttacgagg gcggttttgt caacggcagg cccagcggcg
     6781 aaggccacct gaaaggcagc cagatggaat accgcggcga gttcagcaag ggccagtaca
     6841 gcggcctggg caaactgcag tggagcaacg gcgccagctt tcagggccag ttcaagaaag
     6901 gcgaacccga cggccagggc gtgctgaccg acgccgaagg caatgagtac accggcacct
     6961 tcgataacag ccaactcaat ggtcaaggca gcttcaaaaa tgccgatggc gatctctaca
     7021 gcggccagtt ccgcgataac cagtttcacg gtaagggccg ttaccagagc gccggcggcg
     7081 aagtctggag cgggcgcttc gtccatggca gcctcaccgg caaaggcgaa ttcaagggta
     7141 gcgatggcag ccattacgtc ggtcagttca gcgattggac ctatcacggc gaaggtcgcc
     7201 tgaacctggt cgacggcagc ctctatcaag gcaactttgc ctatggccaa taccaaggca
     7261 tcggcagcct gacgctggtc gatggcagcc agcaaagcgg cacctggcag aacggccggc
     7321 tgacgcgtga cgcccagggc aaggccatac ctgacgccct ggccaccggg ctgctagaac
     7381 aaggtcgcct gctggatgcg gccatcgcca aattgcccgc ctcgaccccg gccatcgagc
     7441 tgtacgccct gaccctggcc ggcgacggca agcagagcgt gtttatgcgc gaggccgatt
     7501 atgtcggcaa cctgctgcag gagcgcttcg ccgcccacgg cctgatcacc ctggtcaatc
     7561 accgcgacca cctggcggat cgcccactgg ccaccagcgc cagcctcgac cgcagcgtcc
     7621 aggccctggc tgagcgcagc ggcaaggaag acctgatctt tatctacctg accagccacg
     7681 gctcggccga gcatgagctg aacctcgacc aaccgcgcct gcagctcaac gacctgcccg
     7741 ccagcgatct ggcagccttg ctcgagccct tgcaggaccg gcacaaggtg ctggtgatct
     7801 cggcatgcta ctccggcggt tttattccca agctgcagga tgacaagacc ctggtaatca
     7861 ccgccgcacg ggccgaccgg gtgtccttcg gctgctcgga ggagaacgat ttcacctact
     7921 tcggccgcgc cctttttgcc gaggccctgc aacagaccga cgacctgcag cgcgccttcg
     7981 aactggccca gaccagcgtc gctgcacgcg aaaaggccga cggcttcgaa ccctcagaac
     8041 cgcagatctg gcccgccaaa gcagtgctcg cacagtggcg caagctgcgc gaacagcagg
     8101 ccgagcgcgc cctgaacaat gcacttgaag tgcaatctgc ggtcaaccca taacagccat
     8161 gcgcgcgccc aacggcctga taagctgagt gatatcagcg gagaacttca cactatgtat
     8221 ttgacgccac agcacatcct tctcgcaggc gccaccggac tgaccggcga gcacctgctg
     8281 gaccgcctgc tgaacgagcc cacagttgct cgcgtactgg cccccagccg tcgcccgctg
     8341 gcagcgcacg cgcacttgga aaacccgcaa ggcgaactgc tggctctgct gccgcaacta
     8401 agcggcaagg ttgatactgc cttctgctgc ctgggcagca cgatcaaaca ggcaggctcg
     8461 caggaggctt ttcgcgcggt cgatcatgac ctggtgatcg ccttcgccga acgcgcccgc
     8521 gccctcggtg cacgccacct gctggtgatc agcgccctcg gcgcggatgc caattccaca
     8581 gtgttctaca acaaggtcaa aggtgaaatg gaacaggccc tgcgcgcgca gggctggccg
     8641 caactgacca tcgttcgccc ttccctgcta ctcggcccac gtcaggagtt tcgccttggc
     8701 gagcgcctgg ccgcaccctt tatgcgctgg ttaccaggca aataccgcgg catcgaagcc
     8761 accgtattgg cccgcgccct gtggcgcctg gcgctggaag aagatgccgg cacccgggtg
     8821 atcgaatcag accaactgcg ccgtctcgtt cgctaacaac cgtccataca gaagccatcg
     8881 acctatgagc agtaccccgc acgacctgat cagcgccctg agcggcgccg acatgctgga
     8941 aatcgacgaa ctgcatgcct ggcagttcag cctcaacgcc gaacaactgg cccagcacca
     9001 ggccggcagc ccgccgactg gtgacgcacc gctgctcagc atcgaatgca tggatggccg
     9061 cgccctgcgc aaatggcatt tcagcctggc ccaggtaatc gccgcacgtt tcgacggcga
     9121 agctgacgcc tggcgcatca gcggcgctgc ctacacgcat ctgatcaagt gctttgccgc
     9181 cgtcagcggc gacaacagtg atatgccaga cgacgccagt aacaacgcag aataaaaata
     9241 atggggacaa caccttgggc ctgtatgacc gctatctgct gccgcatctg atcgatttcg
     9301 cctgcggcat gggcgcagtg atgaaagccc gctcgcaact ggtgccccag gcccatggcc
     9361 gggtgctgga aatcggcatt ggcagcggcc ttaacctgtc gttctatgac gcgtccaagg
     9421 tcagcgtaat tattggcgtc gaccccagcg ccgatatgca gaagctggcc aagcaacggg
     9481 ccgccgcgat cagtattccg gtggagatga ttgccctcga actggggcag atccgggccg
     9541 aggatgccag cttcgactgc atcgtctgca cctttaccct gtgcaccatc cccgatgctg
     9601 tgaccgcgct gaaagaaatg cgccgcgtgc tcaaacccgg agggcgcctg ctgttctgcg
     9661 aacacggcct ggccccggaa ctgcctgtac tgcgctggca gaaccgcctg acaccacttt
     9721 ggaagccact ggctggcggc tgccacctca accgcgacat caaggcgctg atcaccgcgg
     9781 gtggctttca aatcggcgag ttaagcaatc gctatctcaa aggcccaagg cccatgacct
     9841 atatctatca aggctgggct caatagccgc ataaatagcc aattgccatt acccgccagc
     9901 acctgcttca tattctgact atcgttaggc aatggacttt cgtatagaag ttggctcacg
     9961 catgaagcgc ctatcatttc accacctcac caccctactg ctattcatcg cgagccttac
    10021 ggctgtctcg ctgagcggtg cgcaatcacg ccaggtcagc gtgggggttt acgccaacga
    10081 gccgaaaatc ctcctgggcc aggacggcaa actgtccggc atcctcggcg agctgctgaa
    10141 tgaaatcgct cagcaggaag actggcaatt gcgccccaga ccctgtgagt ggcaagaatg
    10201 cctagagctg gcgcagagcg gtgaaatcga cctgatgccc gatgtggcct acaacgacag
    10261 ccgcgcccag gtgctcgact tccacaaagt cccatcactg gtcagctggt cgcaggtcta
    10321 cagcagcgaa aacctgcagc tcagatcgat actcgacctc aaaggcctac gcattgccgt
    10381 gttgcagggc tcggtgcagg aagaatacct acgcgggctg ctcacaagtt ttggtctgaa
    10441 agcccaattg atcggtgtac ccagcctgcc acagggcttt gagcaggtgg ctgccgggca
    10501 ggctgacgcg gtggtggcta accaacgctt tggcgacttc catgcgccga gctatcaact
    10561 gcaaagctcc tcgatcatgt tccaacctgc gcacctgttc tacgccacgc gcaaagggca
    10621 gaacgccgac ctgttagccg ccatcgacca gcacctggcg aactggcagg actcgccctc
    10681 ctcgatctac taccagagcc tgcaacgctg gggcggtgag cggccacgca tgctgatccc
    10741 cggcagcgtc tggtggggtc tgagcactct cgctctgttg ttgatcgccg ccctcggtgg
    10801 cgcgctgttg ttacgccgcc aggtaggcga gaaaacccgt cacctgctgg ccagcgaaga
    10861 acgcctgaac accatcctcg acagcgtcga agcctatatc tatatcaagg atgcgcagtt
    10921 gcgttaccag tacgccaacc gcaaggtctg cgagctgttc ggccagaagc ttgagcaggt
    10981 gatcggcaag accgatgaac agttcttcga tatcgacacc gccaccaacc tacacgataa
    11041 cgaccgccgc gtgctcaaac tcggcgaacg ggtgcagact gaggaaatca accgcagcct
    11101 ggacggcagt actcagcacg ccttcctctc ggtcaagctg cccttgcgcg acggccaagg
    11161 caatatctac gcgctgtgcg gcatctccac cgacatcact gaacacaaga agaacctgga
    11221 aaaaatcaac cagctggcct tctatgaccc cctcaccggc ctgcccaatc gccgcctttt
    11281 gctcgaccgt ctgcagcagg cgctggccaa gcacgcacgc ggcctgcaac agggcgcgct
    11341 gctgtttatc gacctggaca acttcaagga cctcaacgac accctaggcc atgacatggg
    11401 cgaccagttg ctgcaacagg tcgcccagcg cctgagcagc cacgtgcgcg cccaggacac
    11461 cctggcccgc ctcggcggcg atgagtttgt gttgatgctc gaaggcctca gcctgaaacc
    11521 cgcgcaggcg ggccaacagg tcgagagtgt cagccggaaa ataattgaag cgctggcggc
    11581 gccctatgcc ctgcaaagcc gcagccatac cagcacggcc agcataggca tcgcgctatt
    11641 tccccacgag cgcagcacgg tcgatgaagt actcaagcgc gccgatatgg ccatgtatca
    11701 ggccaaagct gccggccgca acgccatgcg ttttttcgac ccgacgatgc aggccgacat
    11761 caacgcccgc gccagcctgg aaagcgacct gcgccagagc cttggccaac gcgactttat
    11821 cctgcactac cagccccagg tggatgccca ggggcagctg gtcggcgccg aagccctggt
    11881 acgctggcaa caccccacac gcgggctggt gccgccgggc gagtttattc cgctggcaga
    11941 aagcaccggg ctgattctgc cgctgggccg ccttatcctg cacagcgcct gccagcaact
    12001 ggtggcctgg gccgcgcacc cgcaactggc gcagctaacc ctggcagtca atgtcagtgc
    12061 caggcagttc caccatcccg actttgtcga agacgtcttc gccactctcg cagaaagcgg
    12121 cgccaaccca cagcgcctag aactggaact gaccgaaagc cagctggtgg aagatgtgga
    12181 agtgctgatc ggcaagatga gccagctgaa agcgcgcggc atacacctgg cgctggatga
    12241 tttcggcgcc ggctactcct cgctcaacta cctcaagcgc ctgccgctgg atcagctgaa
    12301 gatcgaccag tcgttcgtcc gcgatctgct cgaacacccc agcgacgccg ccatcgtgcg
    12361 caccatcctg gcgctgggcg ccaacctcaa cctggccgta accgccgaag gggtggaaac
    12421 ccaggaccag tacgaggccc tgcagcgcat gggctgcagg cgttttcaag gctacctgtt
    12481 cgccaagccg gggccggcgt cagcgcttga gcaatggcat aacctgacgc ccactgaata
    12541 ggcacgctca gggctgctct ccacgcaact gcagggcatc cagcaaaccg gcggcgatgg
    12601 ccatgccgac cagatcggcc agggtgtcgg cctgcatgcg cttcatcacc cgtgagcggt
    12661 acaggtcgat ggtcttgacg ctgatatcca gctgctcggc catctcgcgg ttggtgtagc
    12721 cgcgcaccag cggcagcagc acatcgcgct cacgcggggt caggctggcc agacgctgct
    12781 cgaccgcctg caggcgcggg tccgcctgcg cagctgccgg cgcatggctc agggcctgct
    12841 gcacgctgtc gagcagcagc tgttcgttgt aaggcttttc gataaagtcc acggcgccag
    12901 cacggaacgc ccgcaccaca ataggcacat cggcatgccc actgacaaaa atgatcggca
    12961 tctggatgcc ccggctgcgc atcgcttcct gcacgttcaa gccgcccatg ccgggcatcc
    13021 gcacatccag cagcacgcag ccctggctgc gctcatcaca ggcatcgaga aacgcccgcc
    13081 cgctggtgaa cggcagcgcc gtcagcccca cggactccag taaccagacg gtggagtcga
    13141 gcatgccctg gtcgtcatcg accacataca cccgttgttg catctgcagg ctcatgccct
    13201 gctcctttat tgttatgcct tggttttagc cgcgaccagt aacggtccca tccagactct
    13261 cagcgtcttc accgcgcaac aacggcagac gacactccag gcgcaggccg ccgccctctc
    13321 ccggctgcgc ctccagcgcg ccgccaaagc cctcgatgat actgcgactc atcgacagcc
    13381 ccagccccag cccttcggcc ttgctggtgt agaacggggt gaagatgtgt tccagctcgc
    13441 cctgcgccac gcccggcccc tgatcccaca cccggacaca gagctgccca tcctgctcgg
    13501 cggcgctgag ttcgatgcgc gacgcctgcc cgggatgagc ctcgcggttg gcatcgatgg
    13561 cattgcgcag caggttgagc agcacctgct cca
//
LOCUS       DASRUM010000101         9475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1887087, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000101 DASRUM010000000
VERSION     DASRUM010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9475
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887087"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1313
                     /locus_tag="VFF22_06875"
     CDS             258..1313
                     /locus_tag="VFF22_06875"
                     /inference="COORDINATES: protein motif:HMM:NF016843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HZX16570.1"
                     /translation="MAVSLSERVLSCVGAVLGLLFSGWLCREVLGSASPWLIAPMGAS
                     AVLLFAVPASPLAQPWSILGGNLISAIVGVSCATWLGHDALVAALAGGVAIAGMFALR
                     CLHPPGGAVALTAVLAGPEVAQLGYHFALYPVGLNSVGLLLIALLFNNALRRQYPHRP
                     LEHGNVHKTRDPIPRDRLGFTPADLDAVLKARGELLDISREDLEEILLQTEARAYQRR
                     FGEVRCGDIMSRDVQTLTEHSSLTEARLSLHEHRLSAMPVLNAQGELQGMLSLHDLLL
                     ADADTQTVSQCMRRQAPTCRADWPVTYLLQMLTDSDVHRLPVVDDHRRLLGIVSQSDL
                     LAALFRMSLQSPASGAG"
     gene            1365..2258
                     /locus_tag="VFF22_06880"
     CDS             1365..2258
                     /locus_tag="VFF22_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZX16571.1"
                     /translation="MSVEARSPSNMRPSTLHLPRGNWATVFECLCAHFPAIAPAVWQE
                     RIARGRVLDAQGQPLGMAAAYREGLKVHYFREVPAETSIPFEETLLYVDEHLLVADKP
                     HFLPVMPAGEYVEQTLQARLAKRLGNADLVPLHRIDRHTAGLVLFSTNRVSRGQYQAL
                     FRLRQMDKCYEALAAALPQLDFPLLRATRLEGAEPFFRMQEVAGEANTETRIEVAQRL
                     GEHWLYRLYPVTGKKHQLRVHLAALGAPILNDPFYPEVTDAPDAPDDYSKPLKLLAKG
                     LSFIDPLTGERRSFESQLSLL"
     gene            complement(2255..2839)
                     /locus_tag="VFF22_06885"
     CDS             complement(2255..2839)
                     /locus_tag="VFF22_06885"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007951221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZX16572.1"
                     /translation="MGYRLSKIYTRTGDACETGLADGRRVTKDHPRVDAMGEVDTLNS
                     HIGLLLADLAEHAAHCPGLNEIIKVLTPCQHRLFDLGGELAMPDYQALQAPEIERLEA
                     AIDRWNEEVGPLENFILPGGSRLIAQAHVCRSLARSAERRCQHLNAVEPIRGVGLAYV
                     NRLSDLLFVAARLIAKRQGINEVLWQAAAKEPQN"
     gene            complement(2842..4860)
                     /locus_tag="VFF22_06890"
     CDS             complement(2842..4860)
                     /locus_tag="VFF22_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX16573.1"
                     /translation="MFLRQRIENLPVGRKLLVALLALLATVLLVANLAFISAAYWISQ
                     ESVAPQALHTLGRLIASPPLSAEALSSPEAAKALLRRLDDYVPLRAAVIYDAEGLNLA
                     QLQRGSKLPLPQQIAEVESWRHNEFRINLLVELPQATGKPGHLLLVASSELPGVFYTG
                     TLTASLVILALSVLFWLIVSRQVRRLITRPIRRLEELSRQVTREENYALRARRGNHDE
                     IGSLADAFNTMLVRIEAREQQLKRARDDAQEAFDQAQHLAEETRHSNRKLELEVQVRG
                     KIEKKLTGFQKYLNSIIDSMPSALIALDEQLYVTQWNQEASSLSGTSLDEALNQPIFL
                     AFPPLKPFLPQLRRTVEQHQVEKIERVTWGSNEQPRHFALTFYPLTGGSGRGVVIRID
                     DITQRLNLEEMMVQSEKMLSVGGLAAGMAHEINNPLGAILHNVQNIRRRLSPELEKNR
                     EQAEQAGICLEAIDRYLNAREIPRLLDGIQQAGSRAAKIVSHMLNFSRRSDRQLAPCH
                     LPALVDQALEIASNDFDLTESFDFKSLNIQREFDADLPAVPATANELEQVLLNLLKNA
                     AQAIHQRDDSATPGQMTLRVRLAPPWAEIQVEDNGVGMPESVRKRIFEPFFTTKEVGQ
                     GTGLGLSVSYFIVTNNHKGQMEVQSKAGQGTCFTLRLPMTTATPDTGL"
     gene            complement(4907..5815)
                     /locus_tag="VFF22_06895"
     CDS             complement(4907..5815)
                     /locus_tag="VFF22_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-dehydropantoate 2-reductase"
                     /protein_id="HZX16574.1"
                     /translation="MWHILGAGSLGTLWATRLARAGLPVQLILRDPARLHSYQQAGGL
                     TLIEQGQAQQYAIPAQTSDHPQPIQRLLLACKAYDAAAAVSQLGPRLAPGAEILLLQN
                     GLGSQDEVARATPQARCIFASSTEGAFRQADFQVVFAGHGHTWLGDPLDLQPPALLED
                     LRQAGIVHDWSLDILSRLWRKLALNCAINPLTVLHDCRNGGLREHPAQVACLCSELSE
                     LLHRCGQSAAAEGLHDEVQRVIQATAANYSSMHQDVSQGRRTEIAYLLGYACNAAQRH
                     QLVLPHLQALHSQLLAHLDARGLPSH"
     gene            complement(5828..6793)
                     /locus_tag="VFF22_06900"
     CDS             complement(5828..6793)
                     /locus_tag="VFF22_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX16575.1"
                     /translation="MPTPNLSILVVDDAKFSSAMIGRALTQAGYQDLRFANSAAEAIR
                     LLEQRPASVLLADWLMPEMNGLELTVQVRQFDEMAHHYTYVILLTGKEGENVLGEAFD
                     SGVDDFISKAAMNEQLVPRVYAADRLCNTLQRLLQENRLLTENISSLEQRNLIDPLTG
                     LGNNRYLHQKMLDSLRQVESRGGALCYLLIGLQDMHELHQRHGDSFYNELLTGVARRL
                     QQLVRPLDVLVRLDESHFGLITLLTDLHECSPSSFKRLHEGINLKAFKTSEGFISLKA
                     GISLVGLDAKGLPITPEQITDHAARILPESHSTGLVTALRLSLPQ"
     gene            6944..7423
                     /locus_tag="VFF22_06905"
     CDS             6944..7423
                     /locus_tag="VFF22_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HZX16576.1"
                     /translation="MPSFDVVSELDKHEVTNAIDSAIKELDRRYDLRGKGSFELKELT
                     VNLTADADFQLEQMVGILKLCLVKRKIDVQCLEFKDAYPSGKSVKQEVILREGIEKDL
                     AKKIVAHVKDSKLKVQAAIQGEQVRITGKKRDDLQEAIAALRAKDFGMPLQFNNFRD"
     gene            7516..8343
                     /locus_tag="VFF22_06910"
     CDS             7516..8343
                     /locus_tag="VFF22_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HZX16577.1"
                     /translation="MDMNVEQLVKMSEAWLPVVLEYSGKLTLALITMLIGWWLISKLT
                     SSIGRLLNMRQVDRALSSFICSLVGIVLKVLLLVSVASMIGVETTSFIAVIGAAGLAI
                     GLALQGSLANFAGGVLIMLFRPFRAGDWIEAQGVSGSVDSIQIFHTTLKTADNKVVIV
                     PNGSLSNGHITNYSRESTRRVDINLGIDYSSDIKQAREVLLDIAKDPRVRQDPAPVVF
                     VTGLGDSAINLSLRIWVATPDFWPVTFAFTEQAKERLSEAGIGIPFPQRVVHLVQAN"
     gene            complement(8384..9463)
                     /locus_tag="VFF22_06915"
     CDS             complement(8384..9463)
                     /locus_tag="VFF22_06915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX16578.1"
                     /translation="MAGYRVAALLATAGALYFAEGFGSTALLYQHGAWAGTYLMFALL
                     MLPGLLTSLWMREPDVPLRTQLDAARYGFSHQIASVLVLIVLLVSVPAMFTQFYHTDF
                     ASVINGEASMLDLLLEDRAFLRALLYTILTSLCLSTMGRRGLAPVLTPVNDFILRYRW
                     QAFLLLGLIATYRMSDTVMGVMANVFYIDQGFTKDQIASVSKLFGLVMTLIGAGFGGL
                     LIVRFGIMPILFIGGAASAATNLLFLMLTGMGADLQMLIVTISADNFSAGLATAAFVA
                     YLSSLTNLKFSATQYALLSSIMLLLPRLIGGYSGVMVEKFGYAQFFLVTALLGIPTLL
                     LIILQWRRERPKDSNSAPLTQSEQA"
BASE COUNT         1698 a         2687 c         3162 g         1928 t
ORIGIN      
        1 aggttgccga gctgctgcag ggcttcaatc agccggccta gctgctgggc ggcgttaccc
       61 cggccgatgt agagcgcgcc tggcaggtcg gcgcccagtg cgtggcaggt attcgcgcgt
      121 tctggcccta gtaggtagcg cgttggccgc ctgcgggcaa agagcgttat agtcgcgccc
      181 tcaaatggtg cgcgacaggt gggcgtttaa cggtgcaagg catcaaaaca tggttgaaag
      241 gcttctggcc ggcgcccatg gcggtcagcc tgagcgagcg ggtgctcagc tgtgtgggcg
      301 cggtgctggg cctgctgttc agcggttggt tatgccgcga ggtgctgggc agtgccagcc
      361 cctggctgat cgcccccatg ggcgcgtcgg cggtgctgtt gtttgccgtg ccggccagcc
      421 ctctggcgca accctggtcg attctcggtg gcaacctgat ctccgcaatt gtcggcgtca
      481 gttgcgccac ctggctcggt cacgacgcgc tggtggcggc gctggctggc ggcgtggcga
      541 ttgccgggat gtttgccctg cgctgcctgc atccaccggg tggtgcggtg gcgctgacgg
      601 cggtgctggc cggcccggaa gtggctcagt tgggttatca cttcgccctc tacccggtgg
      661 gactgaactc tgttggtctg ctgctgatag ccctgctgtt caataacgcc ttgcgccggc
      721 agtatccgca tcgtccgctt gagcatggca acgtgcacaa gacccgtgat ccgatccccc
      781 gtgatcgcct gggcttcacc ccggcggatc tggatgcggt gctcaaggcg cgcggcgaat
      841 tgctcgatat ttcccgcgaa gacctggaag agattctcct gcaaaccgaa gcgcgggctt
      901 atcagcggcg ctttggcgaa gtgcgctgtg gcgacatcat gtcccgtgat gtgcagacgc
      961 tgacggagca cagcagtctc accgaggcgc gtttgagcct gcatgagcat cggctcagcg
     1021 ccatgccggt gctcaacgcg cagggcgaat tgcagggcat gctcagcctg catgatctgt
     1081 tgctggccga cgccgatacg cagactgtca gccaatgcat gcgccggcag gcgccgacct
     1141 gtcgcgccga ctggccggtg acctatctgc tgcagatgct cacggatagc gacgtgcatc
     1201 ggctgcccgt ggtggatgat caccgtcgct tgctgggcat cgtcagtcaa tccgatctgc
     1261 tggcggcgct gtttcgcatg tctctgcaga gccctgcgtc aggcgcgggt taagcgcggg
     1321 cctgggttat cctgtgcgct tccgcctggt tgttgagtgt gttcatgtct gttgaagccc
     1381 gttccccctc gaatatgcgt cccagcaccc tgcatctgcc gcgcggcaat tgggcgacgg
     1441 tgttcgaatg cctgtgcgcg catttccctg cgattgcacc ggcggtgtgg caggaacgga
     1501 ttgcccgggg gcgggtgctg gatgctcaag gccagccact ggggatggct gcggcttatc
     1561 gtgaaggact caaggtgcac tattttcgtg aggtgccagc ggagacgtcg attccctttg
     1621 aggaaactct gctttacgtc gatgagcatt tgctggtggc cgacaaaccg cattttctgc
     1681 cggtgatgcc cgctggtgaa tacgtcgaac agaccctgca ggcgcgtctg gccaagcgcc
     1741 tgggcaatgc cgacctggtg ccgctgcatc ggattgatcg gcacaccgca ggcctggtgc
     1801 tgttttctac caaccgcgtc agccgtggtc agtaccaggc gttgtttcgc ctgcgacaga
     1861 tggacaagtg ctacgaggcg ctggcggcgg ctttgccgca gctggacttt ccgctgctgc
     1921 gggcgacgcg gctggagggc gctgagccgt tcttccgcat gcaggaagtc gccggtgagg
     1981 ctaatactga aacccgcatc gaggtcgccc agcgcctcgg cgagcactgg ctttatcggc
     2041 tgtatccagt gacaggcaag aagcaccaac tgcgcgtgca cctggccgcg cttggcgcgc
     2101 cgatcctcaa tgacccgttc taccccgagg tcaccgatgc tccggatgcg ccggatgact
     2161 acagcaaacc gctcaagctg ttagccaagg gactgagctt tattgacccg ctaaccggtg
     2221 agcggcgcag ctttgaaagc cagcttagcc tgctctagtt ttgaggctct ttggcggcag
     2281 cctgccaaag tacttcattg attccctgac gtttggcgat cagccttgcg gcgacaaaca
     2341 gcaggtctga caagcggttg acgtaggcca agccaacgcc gcgaattggc tctacggcgt
     2401 tcaggtgctg acagcggcgc tcagcgctgc gggccaggct gcggcacaca tgggcctggg
     2461 caatcaggcg cgagccgccg ggcaggataa agttctccag cgggccgact tcttcgttcc
     2521 agcggtcgat ggcggcttcc agtcgttcga tttccggggc ctgcagcgcc tggtagtcgg
     2581 gcatcgccag ttcgccgcca agatcgaaca ggcgatgttg gcaaggcgtg agcaccttga
     2641 tgatttcatt caatccgggg caatgggccg catgttcggc aaggtcagcc agcagcagac
     2701 cgatatggct atttagcgtg tccacttcgc ccatggcatc cacccgtggg tggtccttgg
     2761 tcacgcggcg gccatcggcc aggccggttt cgcaggcatc gccggtgcgg gtgtagattt
     2821 tcgagaggcg gtaacccatg ttcagagacc tgtgtcaggc gtggcggtgg tcatgggcag
     2881 gcgcagggtg aagcaggtgc cctggccggc tttggactgc acttccatct gccctttgtg
     2941 gttgttggtg acgataaagt aggaaacaga taagcccagc cccgtgccct ggccgacttc
     3001 cttggtggtg aagaacggct cgaaaatgcg tttgcgcacc gattcgggca tgccgacgcc
     3061 attgtcttcc acctggattt ccgcccaagg cggcgccagg cgtacgcgca gggtcatctg
     3121 ccctggagtc gcgctgtcgt cgcgctggtg aatggcctgg gcggcgttct tcagcaggtt
     3181 gagcagtacc tgttccagtt cgttggcggt ggccggcacg gcgggcaggt cggcatcgaa
     3241 ttcacgctgg atgttcaggc tcttgaagtc gaagctttcg gtcaggtcga agtcattgct
     3301 ggcgatttcc agcgcctgat cgaccagcgc gggcaggtgg cagggcgcga gctggcggtc
     3361 actgcggcgg ctgaaattga gcatatggct gacgatcttc gccgcacggc tgccggcctg
     3421 ctggatgcca tcgagcaggc gcggaatttc ccgagcgttg aggtagcggt cgatggcttc
     3481 caggcagatg ccagcctgtt cggcctgttc gcggttcttt tccagctccg gcgacaggcg
     3541 ccggcggatg ttctgcacgt tatgcaggat ggcccccagc gggttgttga tctcgtgcgc
     3601 catgccggcg gccaggccac cgaccgagag catcttctcc gattgcacca tcatttcttc
     3661 cagattgagg cgctgggtga tgtcgtcaat acggatcacc acgccgcgac cgctgccgcc
     3721 cgtgagcgga tagaaggtta gggcgaagtg gcgcggttgc tcgttgctgc cccaggtgac
     3781 ccgctcaatc ttttccacct ggtgctgctc gacagtgcgc cttagctgcg gcaggaaggg
     3841 cttgagcggc gggaaggcga ggaagatcgg ctgattcagt gcttcgtcca ggctggtgcc
     3901 ggagaggctg ctggcttcct ggttccactg ggtgacgtag agttgttcgt cgagggcgat
     3961 cagcgccgag ggcatggagt caatgatgct gttgaggtac ttctgaaagc cggtgagctt
     4021 cttctcgatc ttgccgcgca cctgcacttc cagctccagc ttacggttgg agtggcgggt
     4081 ttcctcggcc aggtgctggg cctggtcgaa ggcctcctgg gcgtcgtcac gggcgcgttt
     4141 gagctgttgc tcacgggctt cgatacgtac cagcatggtg ttgaaagcat cggccaggct
     4201 gccgatttcg tcgtggttgc cacgccgtgc gcgcagggcg tagttctctt cgcgggtgac
     4261 ttggcgcgag agttcttcca ggcggcgaat tggccgggtg attaggcgac ggacttggcg
     4321 cgagacgatc agccagaaga gtacgctcaa ggccagaatc accaggctgg cggtcagggt
     4381 gccggtgtag aacacccccg gcagttcgct cgaggccact agcagcagat ggccaggctt
     4441 gcctgtggcc tgcggcagtt cgaccagcag gttgatacga aactcgttgt ggcgccagga
     4501 ctcgacctct gcaatctgct gcggaagcgg cagtttgctg ccgcgttgca gctgcgccag
     4561 gttcaggccc tcggcgtcgt agatcaccgc cgcgcgcagt ggcacatagt catccaggcg
     4621 cctgagcagg gcttttgcag cctcggggga actcagtgct tctgcactca gcggtgggct
     4681 ggcaatcagc cggccgagag tatgcagggc ctggggcgca acgctttcct gggagatcca
     4741 gtaggccgca ctgataaaag ccaggttagc taccagcagc acggtagcga gcaaggccag
     4801 tagggcgacc agcaatttgc ggcctacagg cagattttca atgcgttgac gcaagaacat
     4861 ggcgtgctgt cgcgatggtg gaagtgcggg caggttaacg cgggcctcag tggctgggca
     4921 atccgcgtgc atcgaggtgg gccagcagct ggctatgcag cgcctgtaga tgcggcaaca
     4981 ccagttgatg gcgttgcgcg gcgttgcagg catagcccag caggtaggcg atttccgtgc
     5041 ggcggccctg gctgacatcc tgatgcatgg aggaatagtt ggcggcggtg gcctggatca
     5101 cccgttgcac ttcgtcgtgc aggccctcgg cggcggcgct ttggccgcag cggtgcagca
     5161 actcgctcag ctcgctgcac aggcaggcaa cttgcgccgg gtgttcacgc agcccgccat
     5221 tgcggcagtc atgcagcacg gtcagcgggt tgatcgcgca gttcagtgcc agcttgcgcc
     5281 acagccggct gaggatatcc aggctccagt cgtgaacaat gccggcctga cgcaagtctt
     5341 ccagcaaggc ggggggctgc agatcaagcg ggtcgccgag ccaggtatgg ccatggccag
     5401 cgaacaccac ctgaaaatcc gcctgacgaa acgcgccttc ggtgctggag gcaaagatac
     5461 agcgtgcctg gggcgtggcg cgggcgactt cgtcctgact gcccaggcca ttttgcagca
     5521 agaggatttc ggcaccagga gccaggcgag gccccagttg gcttacggca gctgcggcgt
     5581 cataagcctt gcaggcgagc agcaggcgct ggatcggctg ggggtgatcg gatgtttgtg
     5641 ctgggatggc gtactgctgc gcctggccct gctcaatcag ggtcagcccg ccggcttgct
     5701 ggtagctgtg caggcgcgcc gggtcacgca ggatcagctg caccggcagg ccagcgcgcg
     5761 ccaggcgcgt ggcccatagg gtgccgaggc tgccggcacc gaggatatgc cacatgctga
     5821 gcgcggctta ttgcggcaag gacaggcgca gggcggtcac cagtccggtg ctgtgcgatt
     5881 cgggcaatat cctggccgcg tggtcggtga tctgttccgg cgtgatgggc aggccttttg
     5941 cgtccaggcc gaccaggctg atacccgctt tgaggctgat aaagccttcg ctggtcttga
     6001 atgccttgag gttgatgcct tcatgcaggc gcttgaagct gctcggtgag cattcgtgca
     6061 ggtcggtcag cagggtaatc aggccgaaat ggctttcatc cagccgtacc agtacatcca
     6121 gtggccgcac cagctgttgc aggcgccgcg ccacgccggt gagcagttcg ttgtagaagc
     6181 tatcaccatg ccgctggtgt agctcgtgca tgtcctgcag gccaatcagc aggtagcaca
     6241 gcgcgccgcc gcgggattcg acctggcgca ggctgtcgag catcttctgg tgcaggtagc
     6301 ggttgttgcc caggccggtg agcgggtcga tcaggttgcg ttgctccagg ctggagatat
     6361 tttcggtgag caggcggttt tcctggagta ggcgttgcag ggtgttgcac aggcgatcgg
     6421 cggcatagac acgcggcacc agttgctcgt tcattgctgc tttgctgatg aagtcatcga
     6481 cgccgctatc gaaggcttca cccagtacgt tttcgccttc cttgccggtg agcaggatga
     6541 cgtaggtgta gtggtgggcc atttcatcga actggcgcac ttgcacggtt agctccaggc
     6601 cgttcatctc tggcattagc cagtcggcga gcaagacgct agctgggcgc tgttcgagca
     6661 gtctgatggc ttcggctgcg ctgttggcaa accgcaggtc ctgatagccc gcctgggtca
     6721 gggcgcgccc gatcatggcg ctggagaact tggcgtcatc gacgaccaaa atactcaggt
     6781 tgggggtagg catgggcagg ctcgctaggg gaaatcacgt gggcccgata cttcgccggg
     6841 cagtgtatgt tgctaccgct atataatggc cgggtttttt taccgtcaag ccaggcgtgt
     6901 cccgttcgtg atgcagggcg cgccgtctat ctggagtaag cccatgccct cgttcgacgt
     6961 ggtgtccgag ttggacaaac atgaagttac caacgccatc gacagcgcta tcaaagagct
     7021 ggatcgtcgt tatgacctgc gcggtaaagg tagctttgag ctgaaagagc tgactgtcaa
     7081 cctgacagcc gatgcggatt ttcagttgga gcagatggtt gggattctca aactctgcct
     7141 ggtcaaacgc aagatcgacg tacagtgcct ggagttcaag gacgcctatc cgtcgggcaa
     7201 gagcgtcaag caggaagtga tcctgcgcga agggatcgaa aaggatctgg cgaagaaaat
     7261 tgtcgcgcac gtcaaagaca gcaagctcaa ggtgcaggcc gccattcagg gtgagcaggt
     7321 gcgtatcacc ggcaagaagc gtgatgacct gcaggaggcc attgccgccc tgcgtgccaa
     7381 ggacttcggc atgccgttgc agttcaacaa cttccgcgac tgatgcatga gtgtggcgtc
     7441 gcccggtgtt ggtgcagtaa ttccgccacg gcttgccgtg gcgttttatt ttcaaaggtt
     7501 tacaaggatt aacagatgga tatgaacgtc gaacagctgg tgaaaatgtc agaggcctgg
     7561 ctgcctgtgg tgctggaata cagcggcaag ctgaccctgg ccctgatcac gatgctgatc
     7621 ggctggtggc tgatcagcaa actcaccagc agcattggcc gtttgctcaa tatgcgtcag
     7681 gttgaccgcg cgctgagcag ttttatctgc agcctggtcg gcattgtgct gaaggttttg
     7741 ctgctggtca gcgtggcctc gatgatcggc gtggaaacca cttcctttat cgcggtgatc
     7801 ggtgctgccg gcctggcaat cggcctggcg ttgcagggaa gcctggcgaa ttttgcgggt
     7861 ggtgtattga tcatgctgtt tcgcccgttc cgcgccggcg actggattga agcgcagggc
     7921 gtttcgggca gtgtcgacag cattcagatt ttccatacca cgctgaaaac cgcggacaac
     7981 aaggtggtga tcgtgcccaa cggcagcctg tcgaacggtc atatcaccaa ctattcgcgc
     8041 gaatccacgc ggcgtgtcga tatcaatctg ggcatcgatt attcaagcga catcaagcaa
     8101 gcccgcgagg tgttgctgga tatcgccaag gacccacgtg tgcgccagga cccggcgcct
     8161 gtggtgtttg ttaccggctt gggtgatagc gcgatcaacc tgtcactgcg catctgggtg
     8221 gcgacgccgg atttctggcc agtgactttc gcctttaccg agcaggccaa ggagcgcctc
     8281 agcgaggcgg gtatcggcat tccattcccg cagcgggtgg tgcatttggt gcaggcaaac
     8341 taggcacagc gataaagaaa agccccgcat ttgcggggct tttttatgct tgctcagatt
     8401 gtgtgagcgg ggcgctattg ctgtccttcg gtcgctcacg gcgccattgc aggatgatca
     8461 gcagcagtgt cggaataccc agcaacgcgg tgacgaggaa gaactgggca tagccgaact
     8521 tctccaccat tacgccggaa taaccaccga tcaggcgcgg caacagcagc atgatcgaac
     8581 tgagcaaggc gtactgggtg gcggagaact tcaggttggt caggctcgac aggtaggcga
     8641 caaaggccgc ggtggccagg cctgcgctga agttgtcggc ggaaatagtc acgatcagca
     8701 tctgcaggtc cgcgcccatg ccggtgagca taaggaacag caggttggtg gcggccgagg
     8761 cggcgccacc gataaacagg atcggcataa tgccaaagcg cacgatcagc aggccgccga
     8821 aacctgcacc aatcagggtc atcaccaggc cgaacagctt gctaacgctg gcaatctgat
     8881 ccttggtaaa gccctggtcg atgtagaaca cgttggccat cacgcccatc acggtgtccg
     8941 acatgcggta ggtggcgatc aggcctagca gcaggaaggc ctgccagcgg taacgcagga
     9001 taaagtcatt caccggggtc agcaccggcg ccaagccgcg gcggcccatg gtggacaggc
     9061 acaggctggt gaggatggtg tagagcaggg cgcgcaggaa ggcgcggtct tccagcagca
     9121 ggtcgagcat gctcgcttcg ccgttgatca cgctggcgaa gtcggtgtga tagaactgag
     9181 taaacatcgc cggcaccgac accagcagta caatcagcac cagtaccgag gcaatctgat
     9241 ggctgaagcc ataacgggct gcgtccaact gggtacgcag cggtacatcc ggctcacgca
     9301 tccacaggct ggtcagcagg ccgggcagca tcagcagcgc aaacatcagg taggtgccgg
     9361 cccaggcgcc gtgctgatag agcagggccg tagagccaaa gccttcggcg aaatacagcg
     9421 caccggcggt cgccagcagc gcggcgacgc ggtagccggc catatagctg gcggc
//
LOCUS       DASRUM010000102         6996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1723973, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000102 DASRUM010000000
VERSION     DASRUM010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6996
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723973"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VFF22_06920"
     CDS             <1..457
                     /locus_tag="VFF22_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020299273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HZX16579.1"
                     /translation="PLVNGIGSEAQRLSADWGRFMRRELGVEGFRDLARRVEQFLANS
                     ERDSVQVSAHLNDILLAQDYQDLTGQVIKRVTQLVTEVESNLLKLVLMASQVDRFAGI
                     EHDHAVLRAEQEQQKKPSHGEGPQIHADKREDVASNQDDVDDLLSSLGF"
     gene            468..2669
                     /locus_tag="VFF22_06925"
     CDS             468..2669
                     /locus_tag="VFF22_06925"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HZX16580.1"
                     /translation="MSFGADEEILQDFLVEAGEILELLSEQLVELESRPDDMNLLNAI
                     FRGFHTVKGGAGFLQLNELVECCHIAENVFDILRKGERRVDSELMDVVLEALDAVNSM
                     FTEVRERTDLTPATPALLAALARLAEPAGAAQPIPAEAEPEPEAEVEPAAGDITDSEF
                     EQLLGALDESPAQVPSAASSDEITDDEFESLLDQLHGKGQFVAPEVAAAPAAPVAAAP
                     ASSGDEITDDEFESLLDQLHGKGQFVPPPVSAPPAAPAAKAEPAAAPSGDITDDEFEA
                     LLDQLHGKGKFVEPEAIKPVVPAPAAKPVEPAKAAAKPAVKPAAKVEPAKAPAAAVVA
                     PVTEKAAPPSEAETTVRVDTARLDEIMNMVGELVLVRNRLVRLGASSGDEAMAKAVSN
                     LDVVTADLQTAVMKTRMQPIKKVFGRFPRLVRDLARQLKKEINLELVGEETDLDKNLV
                     EALADPLVHLVRNAVDHGIEMPEEREASGKQRGGKVVLSAEQEGDHILLSISDDGKGM
                     DADQLRAKAVEKGLLDKDAADRLNEFECYNLIFAPGFSTKTEISDVSGRGVGMDVVKT
                     KISQLNGTVNVFSVKGQGSKIVIKVPLTLAIMPTLMVMLANQAFAFPLVNVNEIFHLD
                     LSRTNVVDGQEVVIVRDKALPLFYLKRWLIGSAAHVEQGEGHVVILTVGSQRIGFVVD
                     QLVGQEEVVIKPLGKMLQGTPGMSGATITGDGRIALILDVPSMLKRYARRI"
     gene            2713..3828
                     /locus_tag="VFF22_06930"
     CDS             2713..3828
                     /locus_tag="VFF22_06930"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017735229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HZX16581.1"
                     /translation="MAVKVLVVDDSGFFRRRVSEILSADPNIQVIGTATNGREAIEQA
                     QALKPDVITMDYEMPMMDGITAVRNIMQRCPTPVLMFSSLTHEGARVTLDALEAGAVD
                     FLPKNFEDISRNPERVKQLLCEKIHTIARSNRRSLSLPPVAAPTVALAGQAKPAARPT
                     TTTSTLGRPAAAPAPVVASAAPKRKSYKLVAIGTSTGGPVALQRVLTQLPANFPAPIV
                     LIQHMPAAFTKAFAERLDKLCRISVKEAEDGDVLRPGLALLAPGGKQMMVDARGVVKI
                     LPGDERLNYKPCVDITFGSVAKSYADKVLAVVLTGMGADGREGARLLKQSGSQVWAQD
                     EASCVIYGMPMAIVKANLADAIYPLDDIGRHLVETCI"
     gene            3828..4568
                     /locus_tag="VFF22_06935"
     CDS             3828..4568
                     /locus_tag="VFF22_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HZX16582.1"
                     /translation="MDVLSLIGVILAFVAILGGNYLEGGHAGALLNGPAALIVIGGTL
                     GAAFLQAPMNVFKRALAIISWIFFPPRVDLAGGINRVVGWSMTARKEGLLGLEAVADV
                     ETDPYARKGLQLLVDGAEPEAIRSILEVDLYTQEGRDIQAAKVYESMGGYAPTIGIIG
                     AVMGLIHVMGNLADPSKLGAGIAVAFVATIYGVGFANLLLLPIGNKLKAIAQRQSRYR
                     EMLLEGILSIAEGENPRSIELKLQGFMD"
     gene            4581..5450
                     /gene="motD"
                     /locus_tag="VFF22_06940"
     CDS             4581..5450
                     /gene="motD"
                     /locus_tag="VFF22_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HZX16583.1"
                     /translation="MARRRHQEEHENHERWLVSYADFITLLFAFFVVMYSISSINEGK
                     YKILSDSLTGVFNQPDRAIKPIPVGEERPRTTEPDRSLVDEESAQSSADELQSIASSV
                     REAFGDLIQSEQLKVSGNELWIEIELSSSLLFTSGDAIPNNAAFDIIEKIAKILAPYE
                     NPIHVEGFTDNLPIQTAQFPTNWELSTARAASIVRMLTMDGVNPSRLAAVGYGEFQPV
                     ADNTTAEGRGRNRRVVLVISRNLDIRRSVSGVGSANATPDAALQRAGTQPAAAPAAPV
                     PQAGAVNPPSPAP"
     gene            5502..6290
                     /locus_tag="VFF22_06945"
     CDS             5502..6290
                     /locus_tag="VFF22_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HZX16584.1"
                     /translation="MRVWAVANQKGGVGKTTTSIALAGLLADAGKRVVIVDLDPHGSM
                     TSYFGHDPDSLEHSSFDLFQHKGSVPRGLPQQLLLPTSDERISLLPSSTALATLERQS
                     PGQSGLGLVIAKSLAQLWQDFDHAVIDSPPLLGVLMVNALAASQQLVIPVQTEFLAVK
                     GLERMVNTLAMINRSRQQALPYTIVPTLFDRRTQASMGTLRVLRNSYPEHLWPAYIPV
                     DTRLRDASRAGLTPSKFDNNSRGVIAYRALLKHLLSQQAAAQVA"
     gene            6378..>6996
                     /locus_tag="VFF22_06950"
     CDS             6378..>6996
                     /locus_tag="VFF22_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZX16585.1"
                     /translation="MSRPLVTATRPQLALQSYLDGLLQEAAVEFAESITLDEFEAAVL
                     EEQVRDARSMPQAQMQVQATTVKPVVELAPRPLVLPTIEIPAPDIEVHVEPPQEVVKA
                     AVIVEAVQPTPQVPVVPEPALQGRPEWAQGPFECLLFDVAGLTLAVPLVCLGSIYPLE
                     GQELTPLFGQPDWFLGILPCQAGNLKVLDTARWVMPDRYRDDFREG"
BASE COUNT         1404 a         1937 c         2149 g         1506 t
ORIGIN      
        1 accgctggtc aatggcatcg gcagcgaagc gcagcgcctg agtgccgact ggggccgctt
       61 catgcgtcgg gaactggggg tcgagggctt ccgcgacctg gccaggcggg tcgaacaatt
      121 cctcgccaac agcgagcgcg acagcgtcca ggtgtcggcc catctcaacg acattctgct
      181 cgcccaggac tatcaggacc tgaccggtca ggtgatcaag cgggtgaccc agctggtcac
      241 cgaagtggaa agcaacctgc tcaaactggt gctgatggcc agccaggtcg accgctttgc
      301 cggtatcgag catgaccacg ccgtgctgcg tgccgaacaa gaacaacaaa aaaagccttc
      361 ccacggtgaa ggtccgcaga ttcatgccga taagcgtgaa gacgtcgcat ccaatcagga
      421 tgatgtcgac gatctgcttt ccagcctggg attttaggag cacgtctatg agcttcggcg
      481 ccgatgaaga aatcctccag gacttcctgg tggaggccgg cgagattctt gaactactgt
      541 ccgaacagct tgtagagctg gagagtcgcc cggacgacat gaatctgctc aacgctattt
      601 ttcgcgggtt tcataccgtt aaaggtggcg ccggctttct ccagctcaat gagctggtgg
      661 agtgctgcca tatcgcagaa aacgtcttcg acatcctgcg taagggtgag cggcgcgtcg
      721 actccgagtt gatggatgtg gtgctggaag cactggatgc cgtcaatagc atgttcaccg
      781 aagtgcgtga gcgcactgat ctcactcccg ccactccagc gttgctagcc gctttggccc
      841 gcctggctga gcctgcaggt gcggctcagc ccatacctgc tgaagccgaa cctgagcctg
      901 aagcagaagt ggagccggcc gctggcgata taactgacag tgaattcgaa caattattgg
      961 gtgccttgga tgagtcgcca gcgcaagtac ctagtgctgc cagtagtgat gagatcaccg
     1021 atgatgaatt cgagtccttg cttgatcagc tgcatggcaa aggtcagttc gtagcgcctg
     1081 aggttgcagc tgcacctgca gcgcctgttg ccgccgcacc ggcgtcgtct ggcgatgaaa
     1141 tcaccgatga tgaattcgag tcgctgcttg atcagttgca tggcaaaggc cagtttgttc
     1201 cgccgcctgt ttcagcacct cctgctgcgc cagcagctaa agctgaaccg gctgccgcgc
     1261 cgagcggtga tatcaccgac gatgaattcg aagcactgct tgatcagctg catggcaaag
     1321 gcaagtttgt tgagcctgaa gcgatcaagc ccgttgtgcc agcgccagcc gccaagcctg
     1381 tggaaccagc caaggccgct gccaagcctg cggtgaaacc ggccgccaag gtggaacctg
     1441 ccaaggcacc agctgcagcg gttgtagcgc ctgtaactga gaaggctgcg ccgcccagcg
     1501 aagcggaaac cactgtgcgg gtcgataccg ctcggttgga tgaaattatg aatatggttg
     1561 gtgagctggt gctggtgcgt aaccgcctgg tgcgcctggg tgctagcagt ggcgatgagg
     1621 ccatggctaa agctgtgtcc aacctggatg tggtaacggc tgacctacag actgcggtga
     1681 tgaagacccg catgcagcca atcaagaagg ttttcgggcg cttcccgcgc ctggttcgcg
     1741 acctggcgcg tcagctcaag aaagagatca atctggagct ggtcggcgaa gaaaccgatc
     1801 tcgacaagaa cctggtcgag gctctagcag atccgctagt tcacttggta cgcaatgcgg
     1861 tcgaccatgg tatcgaaatg ccggaagagc gcgaggcctc gggcaagcag cgtggcggca
     1921 aggtggtact gtccgccgag caggaaggcg atcacatcct cttgtctatc tccgacgacg
     1981 gcaaaggtat ggatgccgat cagctacgtg ccaaggccgt ggagaaaggc ctgctggaca
     2041 aggacgcggc cgatcgcctc aacgaatttg aatgctacaa cctgattttc gcaccggggt
     2101 tctcaaccaa gactgagatt tctgatgtgt ccggtcgcgg tgtgggcatg gacgtggtga
     2161 aaaccaagat ttcccagctc aatggcacgg tcaacgtatt ttcggtcaag ggccagggtt
     2221 cgaagatcgt tatcaaggtg ccgctgaccc tggcaatcat gccgacgctg atggtgatgt
     2281 tggccaatca ggcgttcgcc ttcccattgg tcaacgtcaa cgaaatcttc cacctcgatt
     2341 tgtcgcgaac caatgtggtc gacggtcagg aagtggtgat cgtgcgtgac aaggccttgc
     2401 ctttgttcta cctcaagcgc tggctgatcg gcagtgccgc ccatgtcgag cagggcgagg
     2461 gccacgtggt gattctcacc gttggcagcc agcgtatcgg cttcgtggtt gatcaactgg
     2521 tcggtcagga agaggtggtt atcaaaccgc tgggcaaaat gcttcagggc actccaggga
     2581 tgtcaggtgc gaccatcacc ggtgatggac gtattgccct gattcttgac gtgccgagca
     2641 tgctcaaacg ctacgcccgg cgtatctgat tgttcgcggc gcaggtatct gtcgcgtcgt
     2701 ttaggagtgt ttatggctgt taaggtgttg gtggttgacg actccggctt ttttcgtcgg
     2761 cgtgtatcgg aaattctttc agccgatccg aatattcagg tgatcggtac agccactaac
     2821 ggccgtgaag ccatcgaaca ggcgcaggcg ctcaaacccg atgtgattac catggattac
     2881 gagatgccga tgatggatgg catcacggct gtgcgtaaca tcatgcagcg ctgcccgact
     2941 ccggtgctga tgttttcctc gctgactcac gaaggcgccc gcgttactct tgatgcactg
     3001 gaagccggtg cggtggactt cctgccgaag aatttcgagg atatctcgcg caacccggag
     3061 cgggttaaac agctgctctg cgagaaaatt cataccattg cccgtagtaa ccgccgtagt
     3121 ctgagtttgc ctcctgtggc ggctcctacg gtcgcgctgg cagggcaagc caagcccgcg
     3181 gcgcgtccta caactaccac cagcaccctg ggtcgtccag ctgcggctcc cgcgccggtt
     3241 gttgccagtg ctgcgcccaa gcgaaaatcc tataagttgg ttgcgatcgg cacctcaact
     3301 ggtgggccgg tggcgctgca gcgtgtgcta acccaattgc cggctaattt tccggcgccg
     3361 attgtgctga tccagcatat gccagcagcc tttaccaagg cctttgccga gcgcctcgac
     3421 aagctctgcc gcatcagcgt caaggaagcc gaggacggtg atgtattgcg ccctggcttg
     3481 gcactgctgg cgcctggtgg caagcagatg atggtcgatg cacgcggcgt ggtgaaaatc
     3541 cttcctggcg atgagcgtct gaactacaag ccctgtgttg atatcacttt cggctcggtg
     3601 gccaaatcct acgccgataa ggtgctggcg gtggtgctga ccggcatggg cgcggacggg
     3661 cgtgaaggcg cgcgcctgct caagcagagt ggcagccagg tgtgggcgca ggacgaggcc
     3721 agctgcgtga tctacggtat gcccatggct atcgtcaaag ccaacctggc cgatgctatt
     3781 taccctctcg atgatattgg caggcacctg gtggagacct gcatttaatg gatgtactga
     3841 gccttattgg ggtcatcctg gcctttgtcg ccattctcgg tggcaactac ctcgaagggg
     3901 ggcatgctgg ggctctgctc aatgggcctg ctgcgctgat cgtgatcggc ggcaccctgg
     3961 gtgcggcgtt tttgcaggcg ccgatgaacg tgttcaagcg ggctctggca atcatcagct
     4021 ggattttctt tccgcctcgc gtggatctgg ctggcggcat caatcgggtg gttggctgga
     4081 gcatgaccgc gcgcaaggaa ggcctgcttg gccttgaagc tgtggccgat gttgaaaccg
     4141 atccctatgc ccgcaaaggc ttgcagctgc tggtcgatgg cgcggagccg gaagccattc
     4201 gcagcatcct cgaagtcgat ctgtacaccc aggaaggccg cgatattcag gctgccaagg
     4261 tgtacgaaag catgggcggt tacgcgccga ccatcggtat tatcggcgcg gtgatgggcc
     4321 tgatccatgt gatgggaaac ttggccgacc cgagcaagct gggcgccggc attgctgtgg
     4381 cgtttgtcgc caccatctac ggtgtgggtt ttgccaacct gctgctgttg ccgattggca
     4441 acaagctcaa ggccatcgcc cagcgccagt cacgttatcg cgagatgctg ctcgagggca
     4501 ttctgtcgat cgctgagggt gaaaacccgc gctccatcga attgaagctg caaggcttta
     4561 tggattgatg gggacactct atggcccgca ggcgtcatca agaagaacat gaaaaccatg
     4621 aacgttggct ggtctcctat gccgatttca tcaccttatt gttcgcgttt tttgtggtca
     4681 tgtattcgat ctcctcaatc aacgaaggta agtacaagat tctttcggac agcctgaccg
     4741 gggtgttcaa tcagcctgat cgggcgatca agccgattcc cgtgggcgag gagcggccgc
     4801 gtaccactga gccagatcgc tcgctggtgg atgaagaaag cgcgcagtca tccgccgatg
     4861 agttgcagag catcgccagc agtgtgcgtg aggcatttgg tgatctgatc cagtccgaac
     4921 aactcaaagt cagcggcaat gagttatgga tcgagatcga actcagctcc agtctgctgt
     4981 tcaccagcgg tgatgccatc cccaataatg cggccttcga tatcatcgag aagattgcca
     5041 aaattctggc accttacgaa aatccgattc atgttgaagg ctttaccgac aacctgccga
     5101 ttcaaacagc ccagttccca actaactggg agctttctac cgcgcgtgct gcgagtatcg
     5161 tgcgtatgct gacaatggat ggggtgaacc ctagtcggtt ggcggcagtc ggctatggcg
     5221 agttccagcc cgttgctgac aacaccaccg cagaggggcg tgggcgcaat cgtcgggtgg
     5281 tgttggtgat ctcgcgcaat ctagatattc gccgtagcgt gagtggtgtc ggcagtgcca
     5341 atgccacacc cgatgcggcc ttgcaacggg ctggcacgca acctgcagca gccccagcag
     5401 cgccggtccc gcaagcgggt gccgtcaatc ccccgtcgcc ggccccataa gggtggcccc
     5461 gttctcggtc aggctacagc tgtaccggga ggatgataac gatgagagtc tgggcagtag
     5521 ccaatcaaaa aggtggagtc ggcaagacca ccacatcaat cgctctggca ggccttttgg
     5581 ccgatgccgg caagcgtgtg gtgatcgtcg atctcgaccc ccatgggtcg atgaccagct
     5641 atttcgggca cgacccggac agcctagagc acagcagttt cgacctgttt cagcataaag
     5701 gcagcgtgcc gcgcggcttg ccgcagcaat tgctgttgcc taccagcgat gagcgtatct
     5761 ccctgctgcc gtcgagtacc gcgctggcta cgctggagcg tcagtcgccc gggcagagtg
     5821 gcctgggcct ggtgattgcc aagagcctgg cgcagctctg gcaggatttc gaccatgcgg
     5881 tgatcgacag cccgcctttg ctcggcgtgc taatggtcaa tgcgctggca gccagccagc
     5941 agttggtgat tccagtgcag accgagtttc tggccgtcaa aggtcttgag cggatggtca
     6001 ataccctggc gatgatcaac cgctcgcgtc agcaggcgtt gccgtacacc attgtgccga
     6061 ccctgtttga tcggcgtacc caggcctcca tgggcacgct gcgagtgctg cgcaacagtt
     6121 accctgaaca tctgtggccg gcctacattc cggtggatac gcgtctgcgt gatgccagcc
     6181 gggcggggct tacaccgtca aagttcgaca acaatagccg tggtgtaatt gcctaccggg
     6241 cgttgctcaa gcacctgctt tcgcagcagg cagccgcaca ggtcgcctaa aatgaacggc
     6301 tatgctgaag atatagactc aagtctgccg ctagcgcggc cgatagtctg tacagattgt
     6361 tattgcgggt tgtattcatg agtcgtccat tggtcaccgc tacgcgtccg caactggctt
     6421 tgcagtccta tctggacggc ttgctgcagg aggctgcggt cgagtttgcc gaaagcatca
     6481 ccctggatga gttcgaggcg gcggtgctgg aagagcaggt gcgtgatgcg cgcagcatgc
     6541 cgcaagcaca gatgcaggtt caggccacga cggtcaaacc tgtggttgaa ctggcgccgc
     6601 ggccgttggt gctacctact atcgagattc ctgcgccgga cattgaagtc cacgtcgaac
     6661 caccgcaaga ggtggttaag gctgctgtga tagtcgaggc tgtacagcct actccacagg
     6721 tgccagtggt gcctgagccc gctctgcagg ggcggcctga atgggctcaa gggccgtttg
     6781 aatgcttgtt gtttgatgtg gcgggcctga ccctagctgt tccgctggtg tgtctgggtt
     6841 cgatttatcc ccttgaaggc caggagctca caccattatt tggccagcca gactggtttc
     6901 tcggcatact gccgtgccag gccggtaacc tgaaagtgct ggataccgcg cgctgggtga
     6961 tgccggatcg ttaccgcgat gattttcgcg aaggtt
//
LOCUS       DASRUM010000103        19826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_894712, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000103 DASRUM010000000
VERSION     DASRUM010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 19826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19826
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894712"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            516..1871
                     /locus_tag="VFF22_06955"
     CDS             516..1871
                     /locus_tag="VFF22_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HZX16586.1"
                     /translation="MRALNPLWSSIVLAAICTQAQGITLSEAIQSTLDNHPEVHAGAN
                     SRLSSDEEVKIAKGGYLPTVDLLMGYGRENTDSPSTRALGNHNTETLNYGTSELRLRQ
                     MIFDGFNTPNEVERTKAVVNSRAYRLLGTSESLALRTVEVYLDLLMRREMVELARNNL
                     QAHQRINDQIGLRSQQGVGSTADLDQSEARLALAENNLYTEQVNLADAEANFFSAVGR
                     MPDELDSPASLKGSLPESLNEARQAVIDNNPFLKSAQSDVQAAEKQYEVAKSTFYPRV
                     DLELATNADNNTQGDEGHNNGWRAAVVMNYNLFNGMRDKARLQSTAHQINESMDIRNN
                     ALRVLNENLSLAWNAMENARLQTPKARDYADYTARVREAYQQQFSLGQRTLLDLLDSE
                     NELFTANRRYSEVRFSEEFSMYRVTAAMGDLLRQQQVVVPAEAVALTEVKSEARLPAM
                     K"
     gene            1895..4054
                     /locus_tag="VFF22_06960"
     CDS             1895..4054
                     /locus_tag="VFF22_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016985929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HZX16587.1"
                     /translation="MTTMERPGSSSDPRLNHDDPLLDGLLILCKLHGCSASRGSLSAG
                     LPLPEQRLTPELLPRAAARAGLQGRLLQRDLASISTLNLPVLLLLKNGRSAVLRQWGP
                     KNQAQILPCEADGGEQWVSREQLASEYSGQAFFARPRHELEEARSPLVPRIEAWFRDT
                     LKLSRWLYTDAILASLLINLLGLMVPLFVMQTYDRVVPNQATSTLWVLAIGLFIGMGF
                     ELLLKILRSHLLDTAGKKTDVVLSATLFERITGMSMKARPATIGGFAQSIHDFQGLRE
                     FLTAVTLTSLIDLPFSFLMITVIWLIGGWLVVIPLLAFPITALFAFIIQARLRDTVEK
                     SLQLASQRQALLIETLGGLETLKACSAESERQHQWEKTHGALTRLDIHARSLAAIATN
                     GTLFLQQFAGMATIVAGVYIIIAGQLSVGALVACYMLGSRILAPLGQIAGLITRYQQA
                     RLTMTSTDALMALPQERQAKQRPLDRTQLHGALDVRDVTFSYPGQSTPALAGISLRLT
                     AGERVGIIGRSGSGKSTLARLVMAFYSPDKGQILLDNLDLRQLDVADLRHQIGYVAHD
                     LPLLAGSLRDNLTLGARYVSDARMLEVAELTGVVDLARQHPQGFDRPVGERGQLLSGG
                     QRQAVLLARALLLDPPILLLDEPTSAMDNTSEEILRNRLQTWAQGKTMLLITHRASML
                     SLVDRLVVLDNGHIVADGPKEAVIEALRKGRVGPAAV"
     gene            4063..5421
                     /locus_tag="VFF22_06965"
     CDS             4063..5421
                     /locus_tag="VFF22_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HZX16588.1"
                     /translation="MQISTSIRDYFASMRQRKQDTEFMPEVDGAILEDSPWFTRITVW
                     VVSACLIVALIWANFAVLEEVTTGEGKAIPSSKIQVIQNLEGGIVSEIFVREGQIVEK
                     GAVLLRLDDTRFLSNRGEAEADRLALVARVERLTAEAEGRPISMPADILREAPQLAED
                     ELALYQSRQSRLQSEQRTLGEQLRQKTQELAEFRSKAQQYRSSAGLLQQELNMSQPLV
                     ATGAISQVEILRLRRSLVEIRGSLDATNLAIPRAESAMDEIKSKIEESELSFRSDAFK
                     ELNEIRTELQKLTATSVAIDDRVSRTTVVSPVRGVIKQLKVNTIGGVVQPGNDMLEIV
                     PLDDSLLIEAKVRPQDVAFLHPGQKAMVKFTAYDYTIYGGLRANLELISADTITDEEG
                     NSFYLIQVRTDKSHLGSEEHPLLIIPGMVATVDIITGEKSVLDYLLKPVLKARSEAMR
                     ER"
     gene            complement(5427..6041)
                     /locus_tag="VFF22_06970"
     CDS             complement(5427..6041)
                     /locus_tag="VFF22_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011059914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HZX16589.1"
                     /translation="MPRPQCPRCTRPAERCLCALIPCLASRTRVLILQHPSEVRHALN
                     TARLAALGLSNAELLVGEVFESLAALLQQPGYCACLLFPGEQAQSVMEFAGQHNELPL
                     LLVVPDGTWRKARKLLHLNPLLADLPRVVLPAGLQSRYRLRKAPMPGALSTIEAIVSA
                     LNTVEAPVCFDALLKPFEVLIEGQIEAMGEQTFQRNHAKSDASI"
     gene            complement(6137..7702)
                     /locus_tag="VFF22_06975"
     CDS             complement(6137..7702)
                     /locus_tag="VFF22_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016498454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HZX16590.1"
                     /translation="MRQNLPVTQRERTFSANERLISTTDLNSNITYCNDAFVEISGFT
                     REELIGQPHNLVRHPDMPPGVFGHMWETIKQGKPWMGIVKNRSKNGDYYWVSAYVTPV
                     YENGRVVGYESVRSLPDEAQKRRAEKLYARLREGKPPVPKLELWTLDIIQSWPLILAS
                     LLILGAHFVLSGPWLWGFIVAAMFGLGTYQLHAKHSLIKRTLAEHPKAFTSALVALTY
                     TDSRGAQALLDMAMISEEARLQTALTRIEDAGANVKMHATRSADLSRSGAKMLDQQRS
                     ETDQSATAINQMAATIQEVTHNVQSTSHAAEEADRLAKQGLQLASGSLDSMHHMAKAV
                     VDIGQAVNDLANSTQSIGSVADVITSIAEQTNLLALNAAIEAARAGEQGRGFAVVADE
                     VRSLASRTRESTEQIHQIISSLRAGAERAVVTAGKGEEISKQSVDSVESVRTALDGIS
                     EAVSRITGMSQQMASASEEQSHVAEDISRQITQIAQLSDRSAEQAHQGAVIGQELEDM
                     ADYLHSLAERFNR"
     gene            complement(7890..8021)
                     /locus_tag="VFF22_06980"
     CDS             complement(7890..8021)
                     /locus_tag="VFF22_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004884133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA1414 family protein"
                     /protein_id="HZX16591.1"
                     /translation="MNVKLQSALLDLLVALGLCERPKLQPIPVRSNSPDSKSAPSRR"
     gene            complement(8120..10153)
                     /locus_tag="VFF22_06985"
     CDS             complement(8120..10153)
                     /locus_tag="VFF22_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZX16592.1"
                     /translation="MRLAFAAVAVGLWSLADSRALQADEAENSAVELAPLLITSPRST
                     THWLSTPAAVTVVESSSAPGEQNLALDALLARVPGVFSLNRYNLAQGLRPAIRGFGAR
                     GNFGVRGIRVLVDGVPLTMPDGQTELDGLDLGLVERMEVLRGPASVLYGNAAGGVLAI
                     ETREPPTIPSMQLDLSAAGLGYQRLRGEIGGSEGNLGGLLAFNSTSLEGYREHSQAQT
                     NSLTGKLRWYAPTGRLRLNFNAIDNRAEDPGGLTAAEVKADRRQAAPNNLRFDADEYI
                     RQQRLGLVWDGYAAGADEYQLRSYLGHREFGNRLGFATSGQTTFKRYFSGIGGQYSHH
                     RELIGLAHKITSGFDLEAQRDDRRRYDNLLGERGQSRQQQDESALSQGLFIEDQIDLS
                     EQWQATLGLRYDQVRLAVDDYYLADNRDDSGSRSLEDWNYSAGLSYRFTLQQSVYARL
                     ASSFETPTISELGNPQGGGFNSALQPAQAFSRELGIKGEWPSLRYSLALYRIELEDEL
                     VAFSIQSGRNFYRNAGQSRRDGLELSLDWQFADAWRWSAAYAYNRYRFEDYQTTAGDF
                     SGKHIPGIPRQTLFSELAYENQGAYLRLGVTAQARVYANDANSQSAPGHALFNLRLGK
                     RFQVGEQSLEPYLGIDNLLGREYFDNLRINDVNARYFEPGPGRTLYTGLRVLF"
     gene            complement(10158..11888)
                     /gene="aceK"
                     /locus_tag="VFF22_06990"
     CDS             complement(10158..11888)
                     /gene="aceK"
                     /locus_tag="VFF22_06990"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016702618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HZX16593.1"
                     /translation="MPQQWPASAIAALILEGFDDYREQFRQITNGARVRFEQAQWQEI
                     QQASAARIALYEECVTAVSERLRKAYEAEVLMDVAQWPLVKSAYIALIDLRYDDELAE
                     TWFNSIFCGLFSHDQINDGCMFIHTTRPALRVNERAPQTRLYEPKGNVEQALRQIFED
                     FSFEVGYENLERDLARLLKQLHVNLPDWVCKDPDLRIELFASRLYRNKGAYLVGRIFT
                     RDEQWPLVIPLLHREGQGIQIDALITDEAEVSIIFSFTRSYFMVRVGYPAEFVGFLKR
                     ILPGKHIAELYTSIGFYKHGKSEFYRSLINHLATTDDKFVMAPGVRGMVMSVFTLPGF
                     NTVFKIIKDRFSPSKNVDRNTVIEKYRLVKTVDRVGRMADTQEFADFRFPLSKFDPEC
                     LAELLEVAPSTVEVKGDTVLVRHCWTERRMTPLNLYVESASAAQVREALDDYGLAIKQ
                     LAAANIFPGDMLLKNFGVTRHGRVVFYDYDEICFLTEANFRRIPQARYPEDEMSSEPW
                     YSVAPLDVFPEEFPPFLFADIQQRKLFNELHGELYDADYWKSLQEAIRAGKVIDVFPY
                     RRKDQADKEY"
     gene            12090..12986
                     /locus_tag="VFF22_06995"
     CDS             12090..12986
                     /locus_tag="VFF22_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014337076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZX16594.1"
                     /translation="MRLADLIRLLLLAAIWGASFLFMRIVAPVLGSMPTAFFRASLGM
                     LGLLAILLLMRVRWDFNGKLKHCLILGLINAGLPSAMYCMAALVLPAGYSAILNATTP
                     MMGVLIGMLFFAEAMTLSKAAGVAIGLFGVAVLTRTGPVEFDLTLLWGAAACLVATAC
                     YGFAGFLTRRWIGQQGGLDNRLTAFASLCGASLFLLPFFGGSLLIQPPASWGGLDVWL
                     SLAGLGFICTAFAYVLYFRLLSDIGPIKASTTTFLIPPFGVLWGALLLDEPLSWAYLS
                     GGVLIAIALWLVVRASATAKPA"
     gene            complement(13052..13354)
                     /locus_tag="VFF22_07000"
     CDS             complement(13052..13354)
                     /locus_tag="VFF22_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HZX16595.1"
                     /translation="MKFIHQREHLNEDDVVVIECSQTCNIRLMSDANFRSFKNGGRHT
                     YHGGAFDTFPAKITVPSSGFWNITIDTVSRKAISVTRKPTLKHSIKIVRKSTSRLS"
     gene            13495..14532
                     /locus_tag="VFF22_07005"
     CDS             13495..14532
                     /locus_tag="VFF22_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZX16596.1"
                     /translation="MHIHDFHQRLTDLGAKPMHIGRITRAWLRGMPLDTGTRNQKTEN
                     FLPLAVRKALPEISNELDGLARLSSEHPATDGSARLLVELADKQMVESVLLPRDGLCV
                     SSQVGCAVGCVFCMTGKSGLLRQLSTAEIVAQVALARRFRAVKKVVFMGMGEPAHNLD
                     NVLEAINLLGTEGGIGQRNLVFSTVGDPRVFERLPQQQIKPALALSLHTTDAELRQAL
                     LPRAPRIDPEELVELGETYARQIDYPIQYQWTLLKGINDSQEEMDGILRLLKGKYAIM
                     NLIPYNSLKADEFQRPDGDRIVQIVRYLHSRGVLTKVRNSAGQDIDGGCGQLRARATE
                     VLDRRRNRAKT"
     gene            complement(14709..15293)
                     /gene="nfuA"
                     /locus_tag="VFF22_07010"
     CDS             complement(14709..15293)
                     /gene="nfuA"
                     /locus_tag="VFF22_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HZX16597.1"
                     /translation="MSTITITDAAHDYLADLLSKQSTPGIGIRVFITQPGTQYAETCI
                     AYCKPGEQKAEDTALGLASFTAWIDAVSEPFLEDAVVDYATDRMGGQLTIKAPNAKVP
                     MVNEDSPINERISYYLQTEINPGLASHGGEVSLIDVVDDGIAVLKFGGGCQGCGQVDL
                     TLKEGIEKTLLERIPELKGVRDVTDHSIKENAYY"
     gene            complement(15379..16092)
                     /locus_tag="VFF22_07015"
     CDS             complement(15379..16092)
                     /locus_tag="VFF22_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZX16598.1"
                     /translation="MSKGREVGIDGDQYPQWQGDGSWLWDRPLGDTRATLILAHGAGA
                     PMDSDFMQQMAQSLAARGVAVLRFEFAYMAARRLDGKKRPPNPQAKLLEQWREVYAQV
                     RQVRQQVAGPLAIGGKSMGGRMASLLADELGADALVCLGYPFYAVGKPEKPRVAHLAE
                     LRTPTLIIQGERDALGDRQAVAGYTLSEAINLHWLTAGDHDLKPLKSSGFTHQQHMLA
                     AADAIAQFLCAQPARAGVW"
     gene            16198..17637
                     /gene="ccoN"
                     /locus_tag="VFF22_07020"
     CDS             16198..17637
                     /gene="ccoN"
                     /locus_tag="VFF22_07020"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HZX16599.1"
                     /translation="MNTASSSAYNYKVVRQFAIMTVVWGIVGMGLGVFIAAQLAWPEL
                     NFNLPWTSFGRLRPLHTNAVIFAFGGCALFATSYYAVQRTSQATLFAPKLAAFTFWGW
                     QLVIVLAAISLPMGWTSSKEYAELEWPIDILITIVWVSYAVVFFGTIIKRNVSHIYVG
                     NWFFGGFILTVALLHLVNNLEVPVTLTKSYSAYAGATDAMIQWWYGHNAVGFFLTAGF
                     LGMMYYFVPKQAGRPVYSYRLSIVHFWALIAVYIWAGPHHLHYTALPDWAQSLGMVMS
                     LILLAPSWGGMINGMMTLSGAWHKLRTDPILRFLVVSLAFYGMSTFEGPMMAIKTVNA
                     LSHYTDWTIGHVHAGALGWVAMVSIGSLYHMLPKVFGRDEMHSIALINVHFWFATIGT
                     VLYIASMWVNGIAQGLMWRAVNEDGTLTYSFVEALEASHPGFVVRVIGGAIFFAGMLV
                     MAWNVWQTVRHTKAAEMDAAAQFSLEGAH"
     gene            17637..18245
                     /gene="ccoO"
                     /locus_tag="VFF22_07025"
     CDS             17637..18245
                     /gene="ccoO"
                     /locus_tag="VFF22_07025"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HZX16600.1"
                     /translation="MRHEILEKNIGLMALMMVLAVSIGGLTQIVPLFFQDVTNEPVAG
                     LKPYTALQLEGRDIYIREGCVGCHSQMIRPFRAETERYGHYSVAGESVYDHPFLWGSK
                     RTGPDLARVGGRYSDEWHRAHLYNPRNVVPESKMPAYPWLVEGTLSGQDTPAKMKALR
                     LVGVPYSDDDIAGASEAVKGKSEMDALVAYLQVLGTAVKNKR"
     gene            18249..18434
                     /locus_tag="VFF22_07030"
     CDS             18249..18434
                     /locus_tag="VFF22_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HZX16601.1"
                     /translation="MSFDFIDIGTLRGIGTALVLLSFVCVTLWAYSAKRRTAFDQAAL
                     LPFADEPTLAAPRSKTP"
     gene            18431..19369
                     /gene="ccoP"
                     /locus_tag="VFF22_07035"
     CDS             18431..19369
                     /gene="ccoP"
                     /locus_tag="VFF22_07035"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HZX16602.1"
                     /translation="MSTFWSWYITLLTVGTLLALFWLIFATRKSEVHKSPTDQTMGHA
                     FDGIEEYDNPLPKWWFMLFLGTLIFSIGYLVLYPGLGSWKGVLPGYEGGWTQVKQWQR
                     EVDKADELYGPIFAKYAAMPLEEVAKDEKALKMGGRMFATYCSVCHGSDAKGAVGFPN
                     LTDNHWRWGGEPSVIKTTILNGRIGAMPAWTAVIGEDGVQQVAAYVRKDLAGLPLPTD
                     KRFDLEKGAAIFAANCQACHGAQGTGMAMLGAPDLTKPAGWIYGSSLVQLQQTIRHGR
                     NGQMPAQEQYLGNDKVHLLAAYVLSLSRQQQTVTEQ"
     gene            19740..>19826
                     /locus_tag="VFF22_07040"
     CDS             19740..>19826
                     /locus_tag="VFF22_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003193164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase Cbb3"
                     /protein_id="HZX16603.1"
                     /translation="MSTAISPTAYNYKVVRQFAVMTVIWGVIG"
BASE COUNT         4160 a         6059 c         5586 g         4021 t
ORIGIN      
        1 ggccgacgcg ttccgtcgcc agctactgca tagacttcgc cgataacttg gctgacgaca
       61 ccaatcaaag tagccatgat tccatcctca ctacaactgc cgaccaccgc cgacacgtat
      121 gccaaaaaag gtcgaaatac ccttcaagga gagaacaaaa ccactggctg attacaacga
      181 cgtcacactc aaaaacaaaa ccgacaaaaa actgtcaccc tgacccgcgt cgcccaccac
      241 accagaaaga tcccgccccg acttgcacct gaccatctgt tttagtcgcg cacgccacct
      301 aagagcacaa aatgcgcggc ttctggatga aaacaatgtg ctgcttttct cagactgcat
      361 aagacttttt tcttataggc cttgtaacaa ttttttctta gctacgctta aaactgttct
      421 tagctgtgat gttacagagc aagagtaacc aacaagagaa ataagccatt tttttggcga
      481 ctggattttg agaatcttat aaggagagtt accagatgcg cgcactcaac cctttatgga
      541 gcagcatcgt actggcagcg atctgtaccc aggcacaagg catcactctg tcggaggcga
      601 tccagagcac gctggataat cacccagagg ttcatgctgg ggccaacagc cgcctttctt
      661 cggatgaaga agtcaaaatt gctaaaggcg gctatctgcc aaccgtcgac ttattgatgg
      721 gctacggtcg agaaaatacc gacagcccaa gcacccgagc actgggcaat cacaacaccg
      781 aaactctcaa ctacggcact tccgagctac gcctgcggca gatgatcttt gatggtttta
      841 atacgcccaa cgaagtagaa cgcaccaaag cggtagtgaa ctcaagggct tatcgcctgc
      901 tgggcacctc ggaaagcctg gccttgcgta ccgtagaggt ctatctggac ctgctgatgc
      961 gccgcgaaat ggtcgagcta gcacgcaaca acctgcaggc tcaccaacgt atcaatgatc
     1021 agatcggcct acgcagccag caaggtgttg gcagtacggc ggaccttgat caatccgaag
     1081 cgcgtttggc actggccgag aacaatcttt ataccgagca ggtaaacctg gccgacgctg
     1141 aagccaactt cttcagtgcc gttggccgta tgcccgacga acttgattcc ccggcctcgc
     1201 tgaaaggttc attgccagaa agcctcaatg aagcccggca agcggtcata gacaacaacc
     1261 ccttcctgaa atccgcacaa tccgatgtgc aagcggccga aaaacaatac gaggtcgcca
     1321 aatcgacctt ctatccgcgc gttgaccttg agttggccac caacgctgac aacaacaccc
     1381 agggcgacga aggccacaac aatggttggc gtgcggccgt ggtaatgaac tacaacctgt
     1441 tcaacggcat gcgtgacaag gcgcgtctgc aatcgactgc ccaccagatc aatgagtcga
     1501 tggacatccg caacaacgcc ctgcgtgtac tcaatgaaaa cctctccctg gcctggaatg
     1561 ccatggaaaa cgcccgccta caaaccccca aagcgcgtga ttacgcggac tacaccgcac
     1621 gagttcgcga agcctatcag cagcagttca gcctgggcca acgcaccctg ctcgacctgc
     1681 tggacagtga aaacgagcta tttaccgcaa accgccgcta cagcgaagtg cgctttagcg
     1741 aagagttctc gatgtaccgg gtaaccgcag cgatgggtga cttgttgcgt caacagcaag
     1801 tggtcgtacc ggcagaagct gtagccctca ctgaagtgaa gagcgaggcc cgtctgccgg
     1861 caatgaaata gaaccggcat aaggagtagt aaccttgacc accatggagc ggccggggtc
     1921 atccagcgat ccgcgtctta atcacgacga cccgctgctt gatggcttgt tgattctttg
     1981 caaactccac ggctgctcag ccagccgtgg cagcttgagt gcaggcctgc cgttgccaga
     2041 gcagcgcctg actcctgagt tgctcccacg cgctgctgcg cgggcgggtt tgcaagggcg
     2101 tttgttgcaa cgcgatctgg ccagtatttc aaccctcaac cttcccgtac tgctgctgtt
     2161 gaaaaacggc cgcagtgctg tactgcgcca atggggaccg aagaaccagg cacagatact
     2221 gccctgtgaa gccgacggcg gcgaacagtg ggtcagccgt gaacaactgg ccagcgagta
     2281 cagcggccaa gccttctttg cccgcccacg gcatgagctg gaagaggcgc gttcgccact
     2341 cgtaccgcgc atcgaagcct ggttccgcga caccctgaag ctctcgcgct ggctgtatac
     2401 cgatgccatc ctcgccagcc tgctgatcaa cctgctcggc ctgatggtgc cgctgtttgt
     2461 catgcagacc tatgaccgcg tcgtgcccaa ccaggccacc tcaacactct gggtattggc
     2521 catcggtttg tttatcggca tgggcttcga gctgttgctg aaaatcctgc gctcgcacct
     2581 gctcgatacc gccggcaaga aaaccgatgt ggtgctgtcg gccaccttat tcgagcgtat
     2641 caccggcatg tcgatgaagg cccgtccggc caccattggc ggctttgctc agagcattca
     2701 tgattttcag ggcctgcgtg aatttctcac ggccgtcacc ctaactagcc tgatcgacct
     2761 gcccttctcg tttctgatga tcacggtgat ctggctgatc ggcggctggc tagtcgttat
     2821 ccccctactg gcgttcccga tcaccgccct cttcgccttt atcatccagg cgcgcctgcg
     2881 cgacaccgta gaaaaaagcc tgcaactggc ctcgcaacgc caggcgctgc tgatcgaaac
     2941 cctcggcggc ctggaaaccc tgaaagcctg cagcgcggaa agcgaacgtc agcatcagtg
     3001 ggaaaaaact catggcgccc tcacccgcct ggatatccac gcccgctcat tagccgcaat
     3061 tgccactaac ggcacgttgt ttctgcagca gtttgccggc atggccacca tcgtggcagg
     3121 ggtttacatc atcatcgccg gccagctcag cgtcggtgca ctggtggcct gttatatgct
     3181 gggcagccgt attctcgcgc cgcttgggca gattgccggg ctgatcaccc gctatcagca
     3241 ggcgcgcctg accatgacca gcaccgacgc gctgatggct ctgccgcagg agcgtcaggc
     3301 caaacaacgc ccgctggacc gtactcaact gcatggcgcg ctggatgtac gcgacgtcac
     3361 gttcagttac cccgggcaaa gtaccccagc gcttgcagga atcagcctgc gcctgactgc
     3421 cggcgaacgc gtcggcatca ttggccgcag cggctccggc aagagcacct tggcgcgctt
     3481 ggtgatggcg ttctacagcc cggacaaagg acagatcctg ctggataacc ttgaccttcg
     3541 tcagctcgac gtggccgatt tgcgtcatca gattggttac gtagcccacg acctgccgct
     3601 actggccggc agcctgcgcg acaacctgac cttgggcgca cgctatgtca gtgatgcgcg
     3661 catgctcgaa gtggccgaac tgaccggtgt ggtcgacctg gcccggcaac atccacaagg
     3721 cttcgaccgc cctgtgggcg agcgcggcca actgctttcc ggcggacagc gccaggccgt
     3781 actgctggcc cgcgccctat tactcgaccc acccattctg ctgctggatg agcccaccag
     3841 tgcgatggac aacaccagcg aggaaatcct gcgcaaccgc ctgcaaacct gggcacaagg
     3901 caaaaccatg ctgctgatca cgcaccgcgc ttccatgctc agcctggttg atcgcctggt
     3961 ggtgctggat aacggccata ttgttgctga cggccctaaa gaagcggtca ttgaagcgct
     4021 acgcaaaggt cgagttggcc ccgcagcggt ataggaacgg tcatgcaaat aagcacctcg
     4081 attcgcgact acttcgccag catgcgccag cgcaagcagg acaccgagtt catgccggag
     4141 gtcgacgggg ccattctcga agactcgccc tggtttaccc gcatcaccgt ttgggtggtc
     4201 agcgcatgcc tgatcgtggc gttgatctgg gccaattttg ccgtgctgga agaagtcacc
     4261 acgggcgaag gcaaagccat cccctccagc aagattcagg ttattcagaa cctggaaggc
     4321 ggcatcgtca gcgagatatt tgtgcgcgaa gggcagatcg ttgagaaagg cgccgtgctg
     4381 ctacgcctgg atgacacgcg ctttctctcc aaccgcggtg aagccgaagc cgatcgcctg
     4441 gcactggtag cgcgggtcga gcgcttaacc gcagaagctg aaggccgccc catcagcatg
     4501 ccggcggata ttctgcgcga ggccccgcaa ctggccgagg atgagctggc gctctatcaa
     4561 tcacggcaaa gccgtctgca aagcgaacaa cgcaccctcg gcgaacagct gcggcagaaa
     4621 acccaggagc tggccgagtt ccgctccaaa gctcagcaat accgctccag cgcgggtcta
     4681 ctgcaacagg agctgaatat gtcgcagccg ctggtcgcaa ccggtgcgat ctcccaggtg
     4741 gaaatccttc gcctgcgtcg gagtctggtc gaaatccgtg gttcgctgga tgccaccaac
     4801 ctggcgatcc cacgggctga atcggcgatg gatgaaatca agagcaagat tgaagagtcc
     4861 gagctgagct ttcgctcaga tgccttcaaa gagctcaatg aaatccgtac cgaattgcag
     4921 aaactcaccg ccaccagcgt ggccattgat gaccgcgtca gccgcaccac cgtggtctcc
     4981 cccgtgcgcg gggtgatcaa gcagctcaag gtcaatacca ttggcggcgt agtgcaacct
     5041 ggcaatgaca tgctggagat cgtaccgcta gacgacagcc tgctgatcga ggccaaggta
     5101 cgcccgcagg atgtcgcctt cctgcatcca gggcagaagg ccatggtcaa attcaccgcc
     5161 tacgactaca ccatctacgg cggactcagg gccaacctag aactgatcag cgccgacacg
     5221 atcaccgatg aggaaggcaa cagcttctac ctgatccagg tgcgcaccga taaaagccat
     5281 ctgggtagcg aggaacaccc gctgctgatc atccccggta tggtcgccac agtggacatc
     5341 atcaccggtg agaagagcgt tctcgattac ttgctcaagc cagtgctgaa ggcgcgctcg
     5401 gaagccatgc gcgaacgctg aaaggcttat atcgaggcat ccgacttggc gtgattgcgc
     5461 tggaaagtct gctcacccat cgcctcgatc tgcccctcaa tcaacacctc aaagggtttg
     5521 agcaacgcat cgaaacagac tggcgcctcg actgtattca gtgcactaac aatcgcttcg
     5581 atggtggata acgcaccggg catcggtgct ttgcgcaagc ggtaacgtga ttgcaagccc
     5641 gccggcagaa ccaccctggg caaatccgcc aacagcggat tgaggtgcaa cagcttgcgc
     5701 gccttgcgcc aggtgccgtc gggcaccacc aatagcagtg gcagttcgtt gtgctgccca
     5761 gcaaactcca tcaccgattg cgcctgctcg ccaggaaaca gcaaacacgc gcaataacca
     5821 ggctgctgca acagcgccgc aagcgactcg aagacctcac ccacaagcaa ttcggcatta
     5881 ctcaagccaa gtgccgccaa gcgtgcggta ttcagcgcat gccggacctc actcgggtgt
     5941 tgcaggatca gcacccgagt acggctggca agacagggaa tcaacgcaca taaacagcgc
     6001 tcggctgggc gtgtacagcg tgggcattgc gggcggggca tggttttctc ctcagccgcg
     6061 cagtttgcca taagccaaat aaaatcggcc cactctcctg cgaggtgggc cgatagcgat
     6121 gcagccggag ctgtccttaa cggttgaagc gctccgccaa actatgcaga tagtccgcca
     6181 tatcctccag ttcctggcca atgacagcgc cttggtgagc ctgctcggcg ctgcggtcgg
     6241 agagctgggc aatctgggta atctgccggc tgatatcttc ggctacatgg ctttgttctt
     6301 cagatgccga ggccatctgc tgactcatgc cagtgatacg acttacggct tcgctgatgc
     6361 catccagcgc ggtgcgcaca gattccacgc tatcgacact ctgtttagaa atctcctcgc
     6421 ccttgcctgc cgttactacc gcccgttcag cgccggcacg cagtgaagaa ataatctgat
     6481 gaatctgctc ggtcgactcg cgcgtgcgtg aagccagcga acgcacttca tccgccacca
     6541 ccgcaaagcc gcgcccctgc tcgccggcac gcgccgcctc gatggcggcg ttaagcgcca
     6601 gtaggttggt ctgctcagca atcgaggtaa tcacatccgc cacgctgccg atggactgag
     6661 tggagttggc caggtcatta accgcctgcc caatgtccac caccgccttg gccatatggt
     6721 gcatagaatc aaggctgccg cttgccagct gcaaaccctg tttggccaga cgatccgctt
     6781 cttcggctgc gtgcgaggtg ctctgcacgt tgtgcgtcac ctcctgaatg gttgccgcca
     6841 tctgattgat cgccgtagcg gattgatcgg tttcactgcg ctgttgatcg agcatcttcg
     6901 ccccagagcg cgacagatcg gccgaacgcg tggcgtgcat cttgacgttt gcaccagcgt
     6961 cctcaatgcg cgttaacgcc gtttgcaggc gtgcctcttc gctgatcatc gccatatcga
     7021 gcaaggcttg tgcaccacgg ctgtcggtat aggttaaggc aactagagcg ctggtaaacg
     7081 ccttagggtg ctcggcaagc gtgcgcttga tcaagctgtg tttagcatgc agctggtagg
     7141 tcccaaggcc aaacatggcc gcaacaataa agccccacag ccagggaccg ctaagcacaa
     7201 agtgcgcacc gagaatcagc agactggcca aaattagtgg ccatgactgg ataatatcca
     7261 acgtccataa ctcaagtttg ggaaccggcg gtttaccctc acgcaagcgg gcatagagct
     7321 tttccgcacg acgcttctgc gcttcatcag gtaaggagcg tactgactcg tagccaacca
     7381 cccggccatt ttcataaacc ggtgtgacat aggcgctgac ccagtagtaa tcaccgttct
     7441 ttgagcgatt tttaacgatc cccatccagg gcttgccctg cttgatggtc tcccacatat
     7501 ggccgaatac gccgggaggc atatcaggat ggcgcaccag attgtggggt tggccgatca
     7561 gctcttctcg agtaaagccg ctgatctcga caaaggcgtc gttgcaatag gtgatattgc
     7621 tgtttaagtc ggtggtggaa atcagccgtt cattcgcgga aaaagttctt tcccgctgag
     7681 tcacagggag gttctggcgc attggatctt acctggttga gctatttaca taaccgtaaa
     7741 tgtaggtcta gcacagcagt actaaaaagc tggcaaatgt ttgttttaaa aagagttgta
     7801 gctctgcagg gtcattatca ggtcgccacg cagatacggc tagaaactat ctagcctcta
     7861 gcaataaaag agtgcatgtg gcggaaaacc taacgtcgag aaggagccga tttggagtca
     7921 ggcgaattgc tgcgcacagg gattggctgc agttttggcc gctcacacag accaagggca
     7981 accagcagat caagcaaggc gctctgcaac ttgacgttca taaaaaagcc tccagccaag
     8041 aacatcggat aggtgcttaa acacctttta tcagcctagt tcaggctact taaaactgct
     8101 gactgttttg tcacacagac taaaacagca cacgcaaacc cgtataaaga gtacgcccag
     8161 gccctggctc aaaatagcgt gcattaacgt cattaatgcg caggttatcg aaatactcgc
     8221 gccccaatag gttatcgatg cccaggtaag gctccaggct ttgctcaccg acctgaaaac
     8281 gcttgccgag gcgcagattg aataaggcgt gaccgggtgc ggactggcta ttggcatcgt
     8341 tggcatatac ccgtgcctga gccgtaactc cgaggcgcag ataagcgcct tgattttcat
     8401 aagccagttc gctgaacagg gtttgccgtg gaataccggg gatgtgcttg ccggaaaaat
     8461 cgcctgcggt cgtttgataa tcctcgaaac ggtagcggtt ataggcatag gccgcagacc
     8521 agcgccaggc atcggcaaac tgccagtcca ggctcagttc cagaccatcg cggcgcgact
     8581 gcccggcatt gcgatagaag ttacggccgg actggatact gaacgccacc agttcatcct
     8641 ccagctcaat ccggtaaagc gccaggctgt aacgcagcga aggccattcg cctttgatgc
     8701 ccagctcgcg actgaacgcc tgggctggct gcagcgcgct attgaagccg ccaccttgcg
     8761 ggttgcccag ctcgctgatg gtgggcgttt caaaggaact ggccagccgc gcataaacag
     8821 attgctgcag ggtaaagcga taactcagcc ccgcgctgta gttccagtct tccaggctgc
     8881 gtgagcctga atcgtcgcgg ttatcggcca gataatagtc atccactgcc aggcgtacct
     8941 ggtcatagcg cagtcctagc gtggcctgcc actgctcgct caggtcgatc tgatcctcga
     9001 taaacagccc ctgactgagc gccgactcat cctgttgttg gcgtgactgc ccacgctcac
     9061 ccaacaggtt gtcatagcgc cgacgatcat cacgctgcgc ttcaagatcg aaaccactgg
     9121 tgatcttgtg cgccaggccg attagctcgc ggtgatgact gtactgcccg ccaatcccgc
     9181 tgaaatagcg cttgaaagtc gtctgtccgc ttgtggcaaa acccagtcga ttaccaaact
     9241 cgcggtgccc caggtaactg cgcagctgat actcatcagc acccgccgcg taaccatccc
     9301 agaccaggcc cagacgttgc tgacgaatat attcatcggc atcaaaacgc agattattcg
     9361 gcgccgcctg gcggcgatcc gccttaacct cggcagccgt taggccgcct gggtcttcgg
     9421 cgcggttgtc aatggcattg aagttcaggc gcaaacgccc agtgggggca taccagcgca
     9481 gcttgccggt gaggctgttg gtttgcgcct ggctgtgttc gcggtagccc tctagagagg
     9541 tgctgttaaa tgccagcaaa ccgcccaggt taccctcgct gccgccgatt tcaccgcgca
     9601 ggcgttgata acccaggccg gccgcgctca ggtccagctg catcgagggt atggtcggcg
     9661 gctcgcgggt ttcgatggcc agcacgccgc cagccgcatt gccgtacagc accgaagctg
     9721 ggccacgcag cacttccatg cgctcgacca ggcccaggtc gaggccgtcg agttcggtct
     9781 ggccgtcggg catggtcaag ggcacgccat cgaccaacac gcgaataccg cgcacaccga
     9841 aattgccgcg tgcgccaaag ccacgaatcg ccgggcgtag cccctgagcc aggttgtaac
     9901 ggttgaggct gaacacaccc ggcacgcggg ccagcaaagc gtccagcgcg aggttttgct
     9961 cacctggcgc cgaactgctc tcaaccaccg tcacagcggc tggggtactc aaccagtgcg
    10021 tggtgctgcg tggcgaggtg atcagcaatg gcgccagctc aacagcgcta ttttcagcct
    10081 cgtccgcctg caatgcgcgg ctatcggcca acgaccatag gccgacggca accgccgcaa
    10141 aggctaggcg cacactctta atattccttg tcggcctggt ccttgcgccg ataggggaac
    10201 acatcaataa ctttgccggc acgaatcgcc tcctgcaggc ttttccagta atcagcgtca
    10261 tacagctcgc catgcagctc gttgaacagc ttgcgctgtt gtatatcggc aaacaaaaac
    10321 ggcggaaatt cctcggggaa tacatccagc ggcgccaccg agtaccaggg ttcggaggac
    10381 atttcatctt ccggatagcg cgcctgaggg atacggcgga aattcgcttc ggtcagaaag
    10441 cagatctcgt cgtagtcgta gaacaccacg cggccgtgac gggtcacgcc aaagttcttc
    10501 agcagcatgt cgccagggaa gatattcgcc gccgccagct gcttgatcgc caggccatag
    10561 tcgtccagcg cttcacgcac ctgcgctgcg ctggcacttt ccacatacag gttgagcggg
    10621 gtcatgcgcc gctcagtcca gcagtggcgc accagcaccg tgtcgccctt aacctcaacg
    10681 gtggacggcg caacctccag cagctcggcc aggcactccg ggtcgaactt gctcagcggg
    10741 aaacggaaat cggcaaactc ctgggtatcg gccatgcgcc ctacccggtc gacggttttc
    10801 accaggcggt acttctcgat cacggtattg cggtcgacgt tcttcgacgg cgagaagcgg
    10861 tccttgatga tcttgaatac ggtattgaag cccggcaggg tgaacacact catcaccatg
    10921 ccacgtacgc cgggggccat gacgaatttg tcatcggtgg tggccaggtg gttgatcagc
    10981 gagcggtaga actccgattt gccgtgcttg tagaaaccga tcgaggtgta caactcggca
    11041 atatgcttgc ccggcaggat gcgcttgagg aagccgacaa actccgccgg gtaaccgacc
    11101 cgcaccataa aataggagcg agtgaaggag aagatgatcg acacctccgc ctcgtcggtg
    11161 atcagcgcat caatctgaat accctgaccc tcgcggtgca gcaagggaat caccagcggc
    11221 cactgctcat ctcgggtaaa gatgcgcccc accaggtacg cgcccttgtt gcggtacagg
    11281 cgcgaggcga atagctcgat acgcaggtcc gggtccttgc acacccaatc cggcaggttg
    11341 acgtgcagtt gcttgagcaa ccgcgccaga tcgcgctcga ggttttcata gccgacttca
    11401 aaactgaaat cctcgaatat ctgccgcaac gcctgctcga cattgccctt gggctcgtac
    11461 aggcgggttt gcggcgcccg ctcattcacc ctcagggccg gtcgcgtggt gtgaataaac
    11521 atgcagccat cgttgatctg gtcatggctg aacaagccgc agaagatcga gttgaaccag
    11581 gtttccgcca gctcatcgtc gtaacgcagg tcgatcaggg cgatataggc cgacttgacc
    11641 agcggccact gcgccacatc catcagcacc tcggcctcgt aggccttgcg caggcgctcg
    11701 ctgactgccg taacacactc ttcatacaag gcaatacgcg ccgctgaagc ctgctggatt
    11761 tcctgccact gcgcctgctc gaaacgcacc cgcgcgccgt tggtaatctg ccggaactgc
    11821 tcgcgataat cgtcgaaacc ttcaaggatc agcgcggcaa tcgcgctggc cggccattgc
    11881 tgtggcataa acgaacccct gcagatgaaa tgcggataat ccaccgagct tatccagtgg
    11941 tcgcgccaag taaaagccgg caatgcctgg atacgcgctt atctgccacg aaatattgcc
    12001 aagcaacggt tttgaaagtg catttcgaat tgccttggcg gatgcgcccg gcaacactgc
    12061 tggcctttct caccgtcgtg gagttcgttg tgcgccttgc cgacctgatc cgtctgctgt
    12121 tgcttgctgc catctggggc gcaagtttcc tgtttatgcg catcgtcgcg ccagtactgg
    12181 gcagcatgcc gactgcgttc ttccgcgcca gtctgggcat gctcggtttg ctggcgattc
    12241 tgttgctgat gcgcgtgcgc tgggatttca acggcaagct caaacactgc ctgatcctcg
    12301 ggttgatcaa cgccggcctg ccttcggcca tgtattgcat ggccgcgctg gttctgccag
    12361 cgggctactc ggcgattctc aatgccacca cgccaatgat gggtgtattg atcggcatgc
    12421 tgttctttgc cgaagccatg accctgagca aggccgccgg ggtggccatc ggcctatttg
    12481 gcgtagcggt gctgacccgc accggcccgg tggagtttga tctcactctg ctctggggcg
    12541 ccgccgcctg cctggtggcc accgcctgct acggctttgc cggttttctc acccggcgct
    12601 ggatcggcca acaaggcggc ctggataacc gtcttaccgc ctttgccagc ctgtgcggtg
    12661 ccagcctgtt ccttttgcca ttcttcggcg gctccctgct gatacagcca cctgctagct
    12721 ggggcggact cgacgtctgg ctgtccctgg cgggcctggg ctttatctgc accgcattcg
    12781 cctatgtgct gtatttccgc ctgctcagcg acatcggccc aatcaaggcc agcaccacca
    12841 ccttcctgat cccgccattc ggcgtgctgt ggggcgcttt gctgctggat gagccgttgt
    12901 cctgggccta cctgtctggc ggcgtgctga tcgccatcgc gctatggctg gtggtgcgcg
    12961 caagtgcaac ggccaagccg gcctgattcg ccccgacaag aaaaagcccg ctatctaagc
    13021 gggcttcagg gactcaaggt caaatcagct atcagctcag gcgcgaggtc gacttgcgca
    13081 cgatcttgat cgaatgctta agcgttggct tgcgcgtcac gctgatggct ttgcgcgaga
    13141 ccgtgtcgat ggtgatattc cagaaaccgc tgctcggtac ggtgatcttc gcggggaaag
    13201 tatcgaacgc tccgccgtgg taggtatgtc ggccaccatt cttgaagctg cggaagttgg
    13261 catcgctcat caagcgaatg ttgcaggtct gcgagcactc gatgacgacg acgtcgtcct
    13321 cgttgaggtg ttcgcgctgg tgtataaatt tcatctaccg ctccgcacag ctgggtcagg
    13381 ccaatcaggc cgctagtatg agtgcccggc gcggcttgag caaaggctaa gcggttttgc
    13441 ctgcgtctcc gacgcatacc accgcaacct ggccggtggt agcatgcgcg ccccatgcat
    13501 attcacgact ttcaccaacg cctcaccgat ctcggtgcca agcccatgca catcgggcgg
    13561 attacccgtg cctggctgcg cggcatgccg ctggataccg gtacgcgtaa ccagaaaacc
    13621 gagaacttcc tgccgctggc ggtgcgcaag gccctgccgg aaataagcaa cgaactcgat
    13681 ggactggcgc gcctcagctc cgaacacccg gcgacagacg gctcggcgcg cttgctggtg
    13741 gagctggccg acaagcagat ggtggaaagc gtgctgctgc cgcgcgatgg cctgtgcgtg
    13801 tccagccagg tcggttgcgc ggtgggctgc gtattctgca tgaccggcaa gagcggcttg
    13861 ctgcgccaac tgagcaccgc cgagatcgtc gcccaggtgg ccctggcacg gcgctttcga
    13921 gcggtgaaga aagtggtgtt tatgggcatg ggcgagccgg cgcataacct cgataacgtg
    13981 ctcgaagcga tcaacctact cggcaccgag ggcggtatcg gtcagcgcaa cctggtgttc
    14041 tccaccgtcg gcgatccacg ggtgttcgag cggttgccgc agcagcagat caagccggcc
    14101 ctggcgctgt ctctgcacac aaccgatgcc gagttgcgcc aggcgctgct gccgcgcgcg
    14161 ccacgtatcg atcctgaaga gctggtcgag ttgggcgaaa cctatgcgcg gcagatcgat
    14221 tacccaatcc agtaccaatg gacgctgctc aagggcatca acgacagcca ggaggaaatg
    14281 gacggtatcc tgcgcctgct caagggcaag tacgcgatca tgaacctgat cccctacaac
    14341 agcctgaaag ccgatgagtt ccagcgcccg gatggcgacc gcatcgtgca gatcgtgcgc
    14401 tatctgcaca gtcgtggcgt gctgaccaag gtgcgcaatt cagcgggcca ggacattgat
    14461 ggcggctgcg ggcaactgcg tgcacgggcg accgaggtgc tggatcggcg cagaaaccgc
    14521 gctaagacgt gaccacgttg gtcctgtcgc ggctaaagcc cctcccacca ggatcagcaa
    14581 cacggccaat cagtctttga ctgcaggctg ttgtgggagg ggctttagcc gcgagccttt
    14641 gcctcaacaa acaaccagga ccataaaaaa tgcccgcaga tcgcgggcat ttttgtttag
    14701 ccatctactt agtagtaggc attttccttg atgctgtggt cagtcacatc acgcacgcct
    14761 ttaagctcgg gaatgcgctc aagcagagtt ttctcgattc cttccttgag ggtcaggtca
    14821 acctggccgc agccctggca gccgccgccg aatttcaata cggcgatgcc gtcgtcaacc
    14881 acgtcgatca ggctgacttc gccgccatgg ctggccagac cagggttgat ctcggtctgc
    14941 aggtagtagc tgatgcgctc gttgatcggg ctgtcttcat tgaccatcgg cactttggcg
    15001 ttcggcgctt tgatggtcag ctggccgccc atgcggtcgg tggcgtagtc gaccacggcg
    15061 tcttccagaa acggctcgct gacggcatcg atccaggcgg taaagctggc caggcccaat
    15121 gctgtgtctt cggccttttg ctcgcccggc ttgcagtagg caatgcaggt ttcggcgtac
    15181 tgggtgcccg gctgagtgat aaatacgcga atgccgatgc ccggggtgct ctgcttgctc
    15241 agcaggtcag ccagataatc gtgggcagcg tcagtaatgg taatggtgct catggcaact
    15301 cctcgcaaac atgcgcgcag tgtacgccac tcacccgcgc cggcaaagtc ctagtgtttt
    15361 agtaggaata gaacttattc accaaacgcc tgccctcgcc ggctgtgcgc agaggaactg
    15421 cgcaatcgca tcggctgctg cgagcatgtg ctgctgatgg gtgaaacctg agcttttcag
    15481 cggtttgagg tcgtgatcac cggcggttaa ccagtgcagg ttgatcgcct ccgatagcgt
    15541 gtagcccgct accgcctgcc gatcccccag agcatcgcgc tcgccctgga tgatcagcgt
    15601 cggcgtgcgc agctcggcta agtgcgcgac gcgtggcttt tccggtttgc cgacggcata
    15661 aaagggataa cccaggcaca ccagcgcatc ggcgcccaac tcatcagcta gcagactggc
    15721 catgcgccca cccatggact tgccgccaat cgccaacggt cctgcgacct gttggcgcac
    15781 ctgccgaacc tgtgcataga cctcgcgcca ttgctcaagc agtttggcct ggggattagg
    15841 tgggcgtttt ttgccatcca gacgccgtgc cgccatatag gcgaattcga aacgcagtac
    15901 ggcaacacca cgcgcggcaa ggctttgcgc catctgctgc ataaagtcgc tgtccatcgg
    15961 cgcaccggcg ccgtgggcca gaatcagggt ggcgcgtgta tcaccaagcg ggcgatccca
    16021 cagccaactg ccatcaccct gccactgcgg gtattgatcc ccgtcaatac ccacctcacg
    16081 ccctttgctc atgcttgcct cgcttaaaga atccgttgaa ctgcggcgct gatcgtacag
    16141 gcccgcagcc gctgattttc gatgatctgc ctataaaccg tggatggaag cccagacatg
    16201 aacacagcaa gcagttccgc ctacaactac aaggtggttc gccaattcgc catcatgacg
    16261 gtggtgtggg gcattgtcgg gatggggctc ggcgtgttta tcgccgcgca actggcctgg
    16321 cccgagctta acttcaacct gccgtggacc agcttcggtc gcctgcgccc gctgcacacc
    16381 aacgcggtga tcttcgcctt cggtggctgc gcgctgttcg ccaccagcta ttacgcggtg
    16441 caacgcacct cgcaagccac cctgttcgcg cccaagctcg cagcctttac cttctggggc
    16501 tggcaactgg tgatcgtcct ggcggccatc agtttgccca tgggctggac cagctccaag
    16561 gaatacgccg agctggaatg gccgatcgac attctgatca ccatcgtctg ggtcagctac
    16621 gccgtggtgt tcttcggcac gatcatcaag cgtaacgtca gccacattta tgtgggcaac
    16681 tggttcttcg gtggttttat cctcaccgtg gcgctgctgc acctggtcaa caacctggaa
    16741 gtgccggtca ccctgaccaa atcctactcg gcctatgccg gggcaacgga cgcgatgatc
    16801 cagtggtggt acggccacaa cgccgtgggc tttttcctca ctgcgggctt tctggggatg
    16861 atgtattact tcgtgcccaa gcaggcaggt cgcccggtct actcctaccg gttgtccatc
    16921 gtgcacttct gggcgctgat cgccgtgtac atctgggcgg gcccgcacca cttgcactac
    16981 accgcgctgc cggattgggc gcagagcctg ggcatggtga tgtcgctgat tctgctggcg
    17041 ccaagctggg gcggcatgat caacggcatg atgaccctct ccggggcctg gcataagctg
    17101 cgcaccgatc cgatcctacg tttcctggtg gtctccctgg cgttctacgg catgtcgacc
    17161 ttcgaaggtc cgatgatggc gatcaagacc gtcaacgccc tgtcccacta caccgactgg
    17221 accatcggcc acgttcacgc tggtgccctt ggctgggtgg ccatggtgtc catcggttcg
    17281 ctctatcaca tgctgcccaa agtcttcggc cgcgacgaaa tgcacagcat cgccctaatc
    17341 aacgtgcact tctggttcgc caccatcggc accgtgctct acatcgcctc gatgtgggtc
    17401 aacggcatcg cccaaggcct gatgtggcgt gcagtcaacg aagacggcac cctcacttac
    17461 tccttcgtcg aagcgctgga agccagccac cccggctttg tggtgcgggt gatcggcggt
    17521 gcgatcttct tcgccggcat gctggtgatg gcctggaacg tctggcagac cgtgcgccat
    17581 accaaggcag ccgagatgga cgccgccgcg cagttttcgc ttgagggagc ccactgatga
    17641 gacacgaaat actcgagaag aacatcggcc tgatggcgct gatgatggtg ctggcggtga
    17701 gcatcggcgg cctgacgcag atcgtcccgc ttttctttca agacgttacc aatgagccgg
    17761 tggcgggcct aaagccctac accgcgctgc agctggaagg ccgtgacatc tacatccgcg
    17821 agggctgtgt cggttgccat tcgcagatga ttcgcccgtt ccgcgcagaa accgagcgct
    17881 acggccacta ctcggtcgct ggcgaaagcg tctacgacca ccccttcctt tggggctcca
    17941 agcgtaccgg gccagatctg gcccgcgttg gcggccgcta ctcggatgaa tggcaccgtg
    18001 cgcacctcta caacccgcgc aacgtagtgc cggaatcgaa gatgccggcc tacccctggc
    18061 tggtagaagg caccctgagc ggccaggaca ccccggccaa aatgaaagcc ctgcgcctgg
    18121 ttggcgtgcc ctacagcgac gacgacatcg caggcgcgag cgaggctgtc aaaggcaaga
    18181 gtgaaatgga tgcactggtc gcctacctgc aagtgctcgg cacagccgtg aagaacaaga
    18241 ggtaggtcat gtcattcgac tttattgata taggcaccct gcgcggcatc ggcactgcac
    18301 tggttctgct gtcgtttgtt tgcgtgaccc tgtgggctta cagcgctaaa cgccgcacgg
    18361 ctttcgatca agccgccctg ctgccctttg ccgatgaacc cacactggct gccccaagga
    18421 gcaagacccc atgagcactt tctggagctg gtacatcacc ctgctgaccg tgggcaccct
    18481 gctcgcgttg ttctggttga tttttgccac ccgtaaaagc gaagttcaca aaagccccac
    18541 cgatcaaacc atgggccatg ccttcgatgg tattgaggaa tatgacaatc cactgcccaa
    18601 gtggtggttc atgctgtttc tcggcacgct gatcttctca attggctatc tggtgctgta
    18661 tcccggcctg ggcagctgga aaggcgtact gcccggatac gaaggcggct ggacgcaggt
    18721 caagcaatgg cagcgtgaag tcgataaggc tgatgagttg tatgggccga tattcgccaa
    18781 atatgcggcg atgcccctgg aagaagtggc taaagacgaa aaagctctaa aaatgggcgg
    18841 acgtatgttt gccacctatt gctcggtttg ccacggctct gacgccaagg gtgcagtggg
    18901 cttccctaac ctgaccgaca accactggcg ctggggcggc gagcccagcg taatcaaaac
    18961 caccatcctc aatggccgta ttggggcgat gccggcctgg acggcggtga ttggtgaaga
    19021 cggtgtgcaa caggtcgcag cttatgtccg caaggacttg gcaggtctgc cactgcccac
    19081 cgacaagcgc tttgatctgg aaaaaggtgc cgctattttt gccgccaact gccaggcttg
    19141 ccacggcgcc caaggcaccg gtatggccat gctgggcgct cccgacctga ccaagccggc
    19201 tggctggatc tacggcagca gcctggtgca actgcagcag accatccgcc atggccgtaa
    19261 cggccagatg ccggcgcagg agcaatacct gggcaacgac aaagtgcatc tactggcggc
    19321 ttatgtactt agcctgagcc ggcaacagca gaccgtcact gagcagtaat cgctaaaacc
    19381 gcaccacctg gctggcgact gacagtcgcc agcccctccc tcccgcgttc ggacaaccgt
    19441 ccggatcatg cgacccaagg tcgcaccctt ccgaccaagt gcactcgcga cgttcgactt
    19501 gagctaatct tgtcgacagt cgccacctgt cataactcca aggcgattcc catctttgcg
    19561 ctacgcctgt ttaggcgttt actgctcgcg gttatgatct gcattagtga ccatgcaggc
    19621 ttaccaacag ccctgtaagc cctatcgcgc cggcatgtgt cgttgcaatg agcacctgct
    19681 ttctccatac ttgccgccga tttttgcccc taaaaaatcc attaaccgtg gaacccataa
    19741 tgagcacagc aatcagtccg actgcttata actacaaggt ggttcgccag ttcgccgtaa
    19801 tgacggtgat ttggggagtc attggc
//
LOCUS       DASRUM010000104         3705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1233061, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000104 DASRUM010000000
VERSION     DASRUM010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233061"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="VFF22_07045"
     CDS             complement(<1..111)
                     /locus_tag="VFF22_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HZX16604.1"
                     /translation="MRHYEIIFLVHPDQSEQVGGMVERYTKLIEEDGGKIH"
     gene            complement(367..1122)
                     /gene="rlmB"
                     /locus_tag="VFF22_07050"
     CDS             complement(367..1122)
                     /gene="rlmB"
                     /locus_tag="VFF22_07050"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HZX16605.1"
                     /translation="MSQLEKIYGVHAVEALLRHHPKRVKQIWLAEGRNDPRVQVLLAL
                     AADARVAVGQCERREMDAWVEGGVHQGVVADVSPSQVWGEAMLDELLDRTEGAPLLLV
                     LDGVTDPHNLGACLRTADAAGALAVIVPKDKSATLNATVRKVACGAAEVIPLVAVTNL
                     ARSLEKLQQRGLWVVGTAGEAEMELYEHDLTGPTVLIMGAEGKGMRRLTREHCDYLVK
                     LPMAGSVSSLNVSVATGVCLFEALRQRKAVGKK"
     gene            complement(1119..3668)
                     /gene="rnr"
                     /locus_tag="VFF22_07055"
     CDS             complement(1119..3668)
                     /gene="rnr"
                     /locus_tag="VFF22_07055"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007902676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HZX16606.1"
                     /translation="MADWQSLDPEAAREAEKYENPIPSRELILQHLSDRGSPASREEL
                     ADEFGMATEEQLEALRRRLRAMERDGQLIYTRRGTYAPVDKLDLILGRVSGHRDGFGF
                     LVPDDGSDDLFLSPAQMRLVFDGDRALARVSGLDRRGRREGAVVEVISRAHESIVGRY
                     YEESGVGFVVADNPKIQQEVLVTPGRNAGAKQGQFVEVKITHWPTTRFQPQGDVVEVV
                     GNYMAPGMEIDVALRSYDIPHVWPEAVVKEARKLKPEVEEKDKEKRIDLRHLPFVTID
                     GEDARDFDDAVYCEKNGSNWRLFSGGWKLYVAIADVSHYVKIDSALDAEAQVRGNSVY
                     FPERVIPMLPEELSNGLCSLNPHVDRLAMVCEISISKTGKMTDYQFYEAVIHSHARLT
                     YNKVSAMLEQPKGSEGKALRGEYKEVLPHLKQLYSLYQVLLAARHERGAIDFETQETR
                     IIFGAGRKIAEIRPTQRNDAHKLIEECMLAANVATAAFMQKHEIPALYRVHDCPPPER
                     VEKLKAFLTELGLSLHRGKSKDGPSPKDYQLLLESIRERPDYHLIQTVMLRSLSQAVY
                     SADNQGHFGLNYEAYAHFTSPIRRYPDLLIHRAIRSVVRSKLDTPHVKRAGATIMPRA
                     RIYPYDDAILEQLGEQCSMSERRADEATRDVVNWLKCEFMKDRVGETFPGVITAVTGF
                     GLFVELKDIYVEGLVHVTALPGDYYHFDPVHHRLAGERSGRSFRLGDSVEVKVMRVDL
                     DERKIDFELAEGKTAMPAGQRRGGFESAAGKTGRRGERAAPGNTDVEKSRALKKSLLS
                     DSKGKTSKSSSAKGDSAKPASKAGKPGAHRKGAPAGGAPASGGARKRKAKS"
BASE COUNT          753 a         1177 c         1037 g          738 t
ORIGIN      
        1 gtgaatcttg ccgccgtctt cttcgatcag cttggtgtaa cgctcaacca tgccgccgac
       61 ttgctcgctc tggtccgggt gaaccagaaa gatgatttcg taatgacgca taaatgctcc
      121 ttacgggttg tagcctgccg ccaaatgcgg tcaggcaagg agtgaatttc acttgtttgt
      181 cttgctgaaa cagaaaggcg caaaagcgcc tgccgtcgcg gcaaggggcg acattctagt
      241 gaagcccccc gccacacgca aggtgaactg gtgattattt gaacaatccg cagcgaatgc
      301 ccggaaagaa aagacgctgc cgaaggctca gcagcaacgg gcccccgtca ggcgcccgcc
      361 acacagttac ttcttgccga cagccttgcg ctgacgcaat gcctcaaaca ggcacacacc
      421 ggtcgcaacc gacacattga ggctgctgac gctgcctgcc atcggcagct tgaccaggta
      481 atcgcagtgc tcacgggtca gccggcgcat gcctttgcct tcggccccca tgatcagcac
      541 ggtcggcccg gtcagatcat gctcgtacaa ctccatctca gcctcaccgg ccgtgccaac
      601 aacccacagg ccgcgctgct ggagcttctc caggctacgt gccaagttgg tcactgccac
      661 cagaggaatc acttcagccg cgccgcaggc gactttgcgc acagtagcgt tgagcgtggc
      721 ggatttgtcc ttcggcacga ttacggccaa cgcaccggcc gcatcggcgg tacgcagaca
      781 agcaccgagg ttgtgcggat cggtcacgcc gtcgagcacc aacaacaaag gggcgccctc
      841 ggtacggtca agcagctcgt cgagcatcgc ctcgccccag acctggctgg ggctgacatc
      901 ggcgactacg ccctgatgca caccaccctc gacccaggca tccatctcgc ggcgctcgca
      961 ctgaccgaca gcaacccgcg catccgcagc caacgccagc agcacttgca cccgcggatc
     1021 attacgccct tcggccagcc agatctgctt gacccgtttc gggtgatgac gcagcaacgc
     1081 ctctacggca tgcacgccat agatcttttc cagctgactc atgacttcgc cttacgcttg
     1141 cgtgcaccgc cagaagccgg cgcaccacct gcaggcgcgc ccttgcgatg cgcgcccggc
     1201 ttgccagctt tgctggccgg cttggccgaa tcgcccttgg cggacgagct tttactggtt
     1261 ttgcccttcg aatcactgag cagcgatttt ttcagcgcac gactcttttc tacgtcggta
     1321 ttacctggag cagcacgctc gccgcgacgc ccggttttac ccgcagccga ttcaaaacca
     1381 ccacggcgct gaccggccgg catagcggtt ttgccttcag ccaactcaaa gtcgatcttg
     1441 cgctcgtcca gatcgacgcg catgaccttg acctcaacgc tatcgcccag acggaaactg
     1501 cgaccactgc gctcgcccgc cagacggtga tgcacggggt cgaaatggta gtaatcgccc
     1561 ggcaaggcgg tgacgtgcac cagaccttcg acgtagatgt ccttcagctc gacgaacagg
     1621 ccaaaaccag tcacagccgt aatcacaccc gggaaggttt cgccgacgcg atccttcata
     1681 aactcgcact tgagccagtt cactacatcg cgggtcgcct catcggcgcg gcgctcggac
     1741 atcgagcact gctcgccgag ctgctcgaga atcgcatcgt cgtatgggta gatccgcgca
     1801 cgcggcatga tcgtcgcacc ggcacgcttg acgtgcggag tgtcgagctt ggaacgaacc
     1861 acgctgcgaa tcgcccgatg gatcagcaga tcagggtagc gacgaattgg cgaagtgaag
     1921 tgcgcgtacg cctcgtaatt caggccgaag tggccctgat tgtcggcgct gtacacagcc
     1981 tgactgagcg agcgcagcat cacggtctgg atcaaatggt aatccggacg ctcgcggatg
     2041 ctttccagca gcagctggta atccttgggc gacggaccgt ccttcgactt gccgcggtgc
     2101 agcgacagcc ccagctcggt gaggaatgct ttgagcttct ccacacgctc cggcggcgga
     2161 cagtcgtgca cgcgatacaa cgccgggatt tcatgctttt gcataaaggc ggcggtggcc
     2221 acgttggcgg ccaacatgca ctcttcgatc aacttgtgcg catcgttgcg ctgggttggg
     2281 cgaatctcgg caatcttgcg accggcgccg aagataatcc gagtttcctg ggtttcaaaa
     2341 tcgatggcgc cgcgctcatg gcgcgcagcc aacagcacct gatacagcga atatagctgc
     2401 ttgaggtgcg gcagtacttc tttgtattcg ccacgcaggg ccttgccttc gctacccttg
     2461 ggctgctcaa gcatggcgct gaccttgttg taggtcaggc gcgcgtggga atggatcacc
     2521 gcttcgtaga actggtaatc agtcatcttg ccggttttcg agatactgat ctcacaaacc
     2581 atggccagac gatcgacatg cgggttcagc gagcacaggc cgttggacag ttcttccggc
     2641 agcatcggga taacccgctc cgggaagtac accgagttac cacgtacctg agcctcggcg
     2701 tccagcgccg aatcgatctt cacatagtgc gatacgtcgg cgatggcgac atacagcttc
     2761 cagccgccgg aaaacagccg ccagttgctg ccgtttttct cgcaatacac cgcatcgtca
     2821 aagtcgcggg cgtcttcacc gtcgatggtg acgaacggca gatggcgcag atcgatgcgc
     2881 ttttctttgt ccttctcttc gacttccggc ttgagcttgc gcgcctcttt aactacagct
     2941 tcgggccaga cgtggggaat atcgtagctg cgcagagcaa cgtcgatttc catgcccggc
     3001 gccatatagt tgccaaccac ttcaaccaca tcgccctggg gctggaagcg cgtagtcggc
     3061 cagtgggtga ttttcacctc aacgaactgg ccctgcttgg cgccggcatt acgccctggc
     3121 gttaccagca cttcctgctg aatcttcggg ttatcagcga cgacaaaacc gacaccgctt
     3181 tcttcgtaat aacggccgac gatgctttcg tgggcgcggg agattacttc aaccacggca
     3241 ccttcacgac gaccacgacg gtcgaggccg gaaacccgcg ccagtgcacg gtcgccatcg
     3301 aacaccaggc gcatttgcgc agggctcagg aacaggtcat cgctgccgtc atctggcacc
     3361 agaaagccaa agccatcgcg atgcccgctg acacgaccca ggatcaggtc gagcttgtcg
     3421 accggggcat aggtgccgcg acgggtatag atcagttgac catcgcgctc catagcgcgc
     3481 agacgacgac gcagcgcttc gagctgctct tcagtggcca tgccaaattc atcggccagc
     3541 tcttcacggc tcgcagggga gccacggtca gacaagtgct gcaggatcag ctcgcgactt
     3601 ggaatggggt tttcgtattt ttccgcttca cgagcggcct cgggatcgag ggattgccaa
     3661 tcggccatta gaggtaattc accttatcta cacaaattcg gtttg
//
LOCUS       DASRUM010000105         3396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2064432, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000105 DASRUM010000000
VERSION     DASRUM010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2064432"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1984
                     /gene="mnmG"
                     /locus_tag="VFF22_07060"
     CDS             92..1984
                     /gene="mnmG"
                     /locus_tag="VFF22_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HZX16607.1"
                     /translation="MDFPSRFQVIVIGGGHAGTEAALAAARMGVKTLLLTHNVETLGQ
                     MSCNPAIGGIGKSHLVKEIDALGGAMAEATDKGGIQFRVLNSRKGPAVRATRAQADRV
                     LYKAAIRETLENQANLWIFQQAADDLIVENDEVKGVVTQMGLRFLADSVVLTTGTFLG
                     GLIHIGLQNYSGGRAGDPPSIALAQRLRELPLRVGRLKTGTPPRIDGRSVDFSVMTEQ
                     AGDTPTPLMSFLGRAEQQPRQVSCWITHTNARTHEIIAANLDRSPMYSGVIEGVGPRY
                     CPSIEDKIHRFADKDSHQVFIEPEGLNTHELYPNGISTSLPFDVQLQIVRSIRGMENA
                     HIVRPGYAIEYDYFDPRDLKYSLETKVIAGLFFAGQINGTTGYEEAGAQGLLAGCNAA
                     LRAQGKDSWCPRRDEAYIGVLVDDLITLGTQEPYRMFTSRAEYRLILREDNADLRLTE
                     KGRELGMVDDVRWAAFETKREAIELEEQRLKSTWVRPGTPQGDAIAAHFGTPLTHEYN
                     LLNLLARPEIDYAGLVTITGGGAHDPQVAEQVEIKTKYAGYIDRQQEEIARLRASEDT
                     KLPLDIDYAGISGLSKEIQHKLGNSRPETLGQASRIPGVTPAAISLLLIHLKKRTAGQ
                     QLEHSA"
     gene            1984..2631
                     /gene="rsmG"
                     /locus_tag="VFF22_07065"
     CDS             1984..2631
                     /gene="rsmG"
                     /locus_tag="VFF22_07065"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HZX16608.1"
                     /translation="MSLVTARHAEELSQGALELGVELSAQQHEQLLAYLALLIKWNKA
                     YNLTAVRDPNEMVSRHLLDSLSVVPFATEAGDNWLDVGSGGGMPGIPLAILFPERQFT
                     LLDSNGKKTRFLTQVKLELKLANLQVIHSRVEEFTPAVPFSGICSRAFSSLEDFSNWT
                     RHLGDGQTQWLAMKGVHPDDELQALPADFRLTSTHVLKVPGCQGQRHLLILRRSA"
     gene            2645..>3396
                     /locus_tag="VFF22_07070"
     CDS             2645..>3396
                     /locus_tag="VFF22_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HZX16609.1"
                     /translation="MAKVFAIANQKGGVGKTTTCINLAASLVATKRRVLLIDLDPQGN
                     ATMGSGVDKHGLEHSIYDVLIGECNLVDAMQFSEHGGYQLLPANRDLTAAEVSLLEMK
                     MKESRLRYALAPIRENYDYILIDCPPALSMLTINALVAADGVIIPMQCEYYALEGLSD
                     LVNSIQRIAEILNPKLKIEGLLRTMYDPRISLTNDVSAQLKQHFPNQLYDVVIPRNVR
                     LAEAPSFGMPALVYDKQSRGAIAYLALAGELVR"
BASE COUNT          702 a         1062 c          989 g          643 t
ORIGIN      
        1 tctttcacac cggctaaatc cctatacttg ccgcctttcc cgcttttcaa cccaaaaagc
       61 cccccatttc tcaacaggca cgaggtgcgt ggtggatttc ccttcccgtt ttcaggtgat
      121 cgtcatcggc ggcggtcatg ccggtaccga agctgcgttg gcagctgcac gcatgggggt
      181 aaagaccctg ctgctgaccc ataacgtgga aacactcggg cagatgagct gcaacccggc
      241 gattggcggg atcggcaaga gccatctggt caaggaaatc gacgccctcg gcggcgccat
      301 ggctgaagcc accgacaaag gtggtattca gttccgtgta ctgaacagcc gcaagggccc
      361 agcagttcgc gctactcgcg cccaagccga tcgcgtcctg tacaaagccg cgattcgcga
      421 aacgctggaa aaccaggcca acctgtggat atttcagcag gcagcggatg acctgatcgt
      481 cgaaaacgac gaagtcaaag gcgtagtcac ccagatgggt ctgcgtttcc tcgcggattc
      541 agtggtgttg accactggta ccttcctagg tggacttatc cacataggcc tgcagaacta
      601 ctccggcggt cgcgctggcg acccaccttc gattgccttg gcgcaacgtc tacgtgaatt
      661 gccgctgcgc gtcggtcgcc tgaaaaccgg tacgccgcca cgcatcgatg gccgttcggt
      721 ggatttctcg gtgatgaccg agcaggcggg cgatacgcca acgccgctga tgtccttcct
      781 cggtcgtgcg gaacagcaac cgcgtcaggt cagctgctgg attacccaca ccaacgcgcg
      841 tacccacgaa atcatcgcgg ccaaccttga tcgttcgccg atgtactccg gcgtgatcga
      901 aggcgtcggc ccgcgctact gcccgtcgat cgaagacaaa atccaccgct tcgccgacaa
      961 ggacagccac caggtattta tcgagcccga aggcctgaat acccatgagc tgtatccgaa
     1021 tggcatctcc acttcgctgc cgttcgatgt gcaattgcag atcgtgcgca gcattcgtgg
     1081 catggagaac gcgcatatcg tgcgtcccgg ctacgccatc gagtacgact acttcgaccc
     1141 gcgtgatctc aagtacagcc tggaaaccaa ggtgattgcc gggctgttct tcgccgggca
     1201 aatcaacggc accaccggtt atgaagaagc cggtgcgcaa ggtttgctgg ctggttgcaa
     1261 cgccgcattg cgcgctcagg gcaaggacag ctggtgcccg cgtcgcgacg aggcctacat
     1321 cggcgtgctg gtcgacgacc tgattaccct gggtacccag gagccgtatc gcatgttcac
     1381 ttcgcgcgcc gaataccggc tgatcctgcg cgaagacaac gccgacctgc gcctgaccga
     1441 gaaaggccgc gagctgggta tggtcgatga tgtgcgttgg gccgctttcg aaaccaagcg
     1501 cgaagccatc gagctggaag aacagcgcct gaaaagcacc tgggtacgcc cgggtacgcc
     1561 ccagggtgat gcgattgccg ctcacttcgg tacgccgctt acccacgaat acaacctgct
     1621 caacctgctg gcccgcccgg aaatcgatta cgcagggctg gtgaccatca ccggcggtgg
     1681 cgcgcatgat ccgcaggtgg ccgaacaggt cgaaatcaag accaagtacg ccggttatat
     1741 cgaccgccag caggaggaaa tcgcgcgcct gcgcgccagt gaagacacca agctgccgct
     1801 cgatatcgac tacgcgggga tttccgggct gtccaaggaa atccagcaca agctcggcaa
     1861 cagtcgtccg gaaactctgg gccaggcctc gcggattccc ggagtcaccc cggcagcgat
     1921 ttccctgctg ctgattcacc tgaaaaagcg cactgccggt cagcagttgg agcacagcgc
     1981 ctgatgtcgt tggtcaccgc acgtcacgcc gaagaactga gccagggcgc tctggagttg
     2041 ggtgtcgaac tcagcgcgca gcagcacgaa cagttgctgg cctacctggc cctgctgatc
     2101 aagtggaaca aggcttacaa cctcaccgca gtacgcgacc cgaatgaaat ggtctcgcgc
     2161 catctgctcg acagcctctc cgtggtgcct tttgccaccg aggctgggga taactggctg
     2221 gacgttggca gcggcggcgg tatgcctggg attcccctgg ccatcctgtt tcccgagcgg
     2281 cagttcaccc tgcttgattc caacggcaag aagacccgct tcctgactca agtgaagctg
     2341 gagctgaaac tggccaacct gcaagttatc cacagccgcg tcgaagagtt caccccggct
     2401 gtgccgttca gcggtatctg ctcgcgggcg ttcagttcgc tggaagactt cagcaactgg
     2461 actcgccatc tgggcgatgg gcagacccag tggctggcga tgaaaggcgt acacccggat
     2521 gacgagctgc aggccctgcc tgcggacttc cgtctcacct ctacccatgt actcaaggtt
     2581 cccggttgcc aaggtcagcg ccatctgctg atactgcgtc gctcggctta gggggaacgg
     2641 cagcatggct aaggtattcg caatcgctaa ccagaaaggc ggtgtgggca aaaccaccac
     2701 ctgcatcaat ctggctgcct cgctggtggc taccaagcgc cgtgtgctgc tgatcgacct
     2761 cgacccccag ggcaacgcga ccatgggcag tggtgtggat aagcacggcc tggagcattc
     2821 gatctacgac gtgctgatcg gcgaatgcaa cctggtcgat gccatgcagt tctccgagca
     2881 cggcggctat cagctgctgc cggccaaccg cgatctgacc gccgccgaag tctcgctgct
     2941 ggaaatgaaa atgaaggaaa gccgcctgcg ttatgcgctg gcgccaatcc gcgaaaatta
     3001 cgattacatc ctcatcgact gcccgccggc gctgtcgatg ctcacgatca acgccctggt
     3061 ggccgccgat ggggtgatta tccccatgca gtgcgagtac tacgcactcg agggcttgag
     3121 cgacctggtc aacagcattc agcgcatcgc cgagatactc aatcccaagc tgaagatcga
     3181 aggcctgctg cgcaccatgt atgacccgcg catcagcttg accaatgacg tttcggcgca
     3241 gctcaagcag cacttcccga atcagctcta cgacgtggtc atcccgcgta acgtgcgtct
     3301 ggccgaggcg ccgagcttcg gcatgccggc gctggtgtat gacaagcaat cccgtggtgc
     3361 cattgcctac ctggcgctgg ccggcgaatt ggtacg
//
LOCUS       DASRUM010000106        12697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1401875, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000106 DASRUM010000000
VERSION     DASRUM010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 12697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12697
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401875"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..1321)
                     /locus_tag="VFF22_07075"
     CDS             complement(302..1321)
                     /locus_tag="VFF22_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16610.1"
                     /translation="MEIESRIKQSRFMNATAILAGVTGIIFILIKDIFDGYETSSPGI
                     ILAIPVALIMMSVSVFLLNYLKSGQIFGEKRNTNEELMREELSILRKELKNNSNFSRH
                     SESLFEEVQALKEKISTRDRNYELLSDEEKIEIISNIKNDILKNASESFLTAIEEKYS
                     TDIRKDKYISDLREQCQQVRARLTREIEALGRRGNLNLVIGVITTISAVTVLATTVLS
                     GNHTLTSNELAAYYAPRLTLSIFIEIFSFFFLKLYKTGLGEIKYFQNELTNAELKFIA
                     AEKAVMANNEESIAQVITELVSTERNFKLGKGESTVDLEKYKSDQASNQKIMESLAGL
                     ISNKK"
     gene            complement(1543..2541)
                     /locus_tag="VFF22_07080"
     CDS             complement(1543..2541)
                     /locus_tag="VFF22_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZX16611.1"
                     /translation="MRERTIASHFVRAALRGAERQGHDCTPLLRQLGIQPALLDEPRA
                     RVAPEQFARLVQLLWESLDDEYLGFGRLRSKRGTFAMMCYAIIHCHSLEKALGRGALF
                     YSLFPEAPAISIQREGDWARLSVDDSTLWDPDHFLVESLLVIWHRLGSWLIGQRIRLE
                     EATFTYSQPAHAGEYELLFPCTRRFTAGRTSLLFHARYLAMPLLQDERTLKQFLQHSP
                     ADLLARPDGGDSLISQIRRLLGRDCSNWPDLDAVAQQLHMSPQTLRRHLREEGSSFQE
                     LKDHLRRDLAIYHLGRDELAIQDIAEQLGFSEPSAFHRAFKKWTGLTPGAYRAQEG"
     gene            complement(2683..4890)
                     /locus_tag="VFF22_07085"
     CDS             complement(2683..4890)
                     /locus_tag="VFF22_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZX16612.1"
                     /translation="MPDTFPAIRPLAALPNRFGYTLLSSLALVLLLVWLLPSSSELKL
                     SHGWFPTWLHTSTEFFAIVVAMLVFSVTWHSYRAERAGNLLILACGFLAVGFLDFAHV
                     LSYRGMPGFITPASPNKAIDFWLAARLVAALCLLTVALRPWTPLRRSRSRYLLLAAAV
                     LLSALFTYLQLWHPGWFPQMFIDGQGLTTLKVRLEWLIITLFALAALLFWRDSLSPQP
                     YDAAGLFLAVALSLLSELCFTAYSNVNDIYSLLGHLYKVLSYWFIYRVVFVSSVHEPY
                     RRLAAEMAERQAAEQKIEVLAFYDSLTGLPNLDLLRDRTDQALVAQHGSAECVALIFM
                     DVDGFKTLNDSLGHSYGDALLRGLAERLRPLLKGGDTLCRSGGDEFIMLLRELEHADD
                     AADMAQQIMQQVRQPFSILGRSINTSLSLGVALAPHDGNSFESLLRNAETAMYKAKQD
                     GRQTWRFFDAAMNDEALEHLTLLNGLRQAIDNHELLLHYQPQIDLRSGALIGVEALVR
                     WQHPELGLLPPGRFIPAAEESRLIVPMGEWILHQACKQAAAWHRAGLSIAQVAVNISA
                     IQLQQGKVEQLVLQALASSQLSAQMLELEITESSLLDKTDQVLDTLKRLKAMGVRLSI
                     DDFGTGYSSLAYLRHLAVDKLKIDQSFVRDLNGSSDSRAIVTAIVQMARSLGLATIAE
                     GVEDNATAHALFELGCFEVQGYLYARPLPAEQLQAFIEAHLPWPLPNGAADNT"
     gene            5118..6257
                     /locus_tag="VFF22_07090"
     CDS             5118..6257
                     /locus_tag="VFF22_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZX16613.1"
                     /translation="MQRLIFDTEHNMFRDAFAAFLKKEVVPHQDEWEAAGVVSREVWK
                     KAGDMGFLLPWADEEYGGAGLKDFRYEQIMCEELAKINEPGFMLPLHSALCGPYIAEY
                     GNAEQKARFMPGIISGELILAVAMTEPSAGSDLAGMRTSAVDQGDHYVLNGSKVFISN
                     GLLADVVIVAAKTDPANKHAMGLFLVERGMDGFERGKNLEKLGMKSQDTAELFFNNVK
                     VPKANLLGDAKGGFFYLMNMLAQERLTNACGAVAAAEAALQVTIDYVRERKAFDRPIS
                     HFQNTRFKLAEMRTQIDVAQVFVDRCVTDHNEKKLTPEVAAEAKLFTTELLCKVVDEG
                     VQLHGGWGYMWEYPICKMYANARIQRIFAGTSEIMKEIISRGMKL"
     gene            6443..8080
                     /locus_tag="VFF22_07095"
     CDS             6443..8080
                     /locus_tag="VFF22_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZX16614.1"
                     /translation="MTDQLPLERLALWVEKRPDAVWLSQPVNGQWHDFTWAQVDDQAR
                     RMATALHALGCAPGDRVALLAKNCAEWIIADLAIMLAGLVSVPLYPLQSAESIDYVLR
                     HSQCKAIFLGKLDEPAKLAPGIPADVMRIAMPYPTIEAQHNWQELLAVHQPLRDGHLQ
                     APDELLSILYTSGTTGQPKGVMLSAKAFAIAGGRSVKELQITEQDQYFSYLPLSHAAE
                     RFLVEMNALYSGGRVAFVESLETFASDLRHVRPTVFFSVPRLWTRFQQGVLEKLPQPK
                     LARLLGIPLIGGLVARKIRKGLGLDRARILVSGAAAIPRALLDWYQSIGITICEGYGM
                     TEHLAYGCFNRPGQVRFGTVGRPMPGNELRIAENGEILLRCPSLMLGYYQDPEKTAET
                     ITDGWLHTGDKGEVDAAGYLKITGRVKDIFKTSKGKYIAPAPIEGEIAKNLWVEQVCL
                     MGSNLDQPLALIELSPAAREHAREQVAADLAQTLQQLNAQLEAHERLSHFVLVREAWT
                     VDNGCMTPTMKIRRNVLEARFADQLDELDAKQPLHWQ"
     gene            8253..9434
                     /locus_tag="VFF22_07100"
     CDS             8253..9434
                     /locus_tag="VFF22_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZX16615.1"
                     /translation="MSGPLSSLKMLDFSTLLPGPFASLLLADMGAEVLRIESPSRMDL
                     VRVLPPQDGGVSASHAYLNRNKRCIALDLKQAEAVAMVKQLVAEYDIVLEQFRPGVMD
                     KLGVGYEALKAINPKLIYVSITGYGQSGPYKDRAGHDINYLALAGIASYTGRRETGPL
                     PLGIQAADIAGGSLHGVIGLLAAVIQRQATGQGQQVDISMTDCAFSLHGMAGAGYLAA
                     GVEPAMENQALNGGSFYDYYRTRDGRWFSVGSLEPQFMQQFCAAIGRPELAARGLSQK
                     PEDQQALKREIEIEFEKRDFAEWGQVFAELDACVEPMLNLSEAVEHPQIKARGLVTEV
                     PRAGNGPQAQIACPIKFSSGLPAPRHIGAVLGAHTAEVLAELGYSAEQIAQLKAAKVV
                     A"
     gene            9538..10155
                     /locus_tag="VFF22_07105"
     CDS             9538..10155
                     /locus_tag="VFF22_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HZX16616.1"
                     /translation="MYHGERFNAWTHLVGAILACAGAVWLLVLASLDGELRKIISVAI
                     YGLTLVLLYSISTLYHSVQGRAKVVMRKLDHLSIYLLIAGSYTPFCLVTLEGAWGWTL
                     FGIVWTLAVIGMLQEIKPRSEARVLSIVIYAVMGWIVLIAVKPLIAALGMTGFIWLAG
                     GGVLYTVGIIFFAYDSRFRHWHGIWHLFVMGGSLMHFVAVLRYVV"
     gene            10394..11071
                     /locus_tag="VFF22_07110"
     CDS             10394..11071
                     /locus_tag="VFF22_07110"
                     /inference="COORDINATES: protein motif:HMM:NF033920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HZX16617.1"
                     /translation="MRVVKLLPIALLFLLSACTSGPQLLPETKRLPERVELADVPFFQ
                     LSDAPGGPSALAALLNHYGVITSPGLVDERIQQLAKGQSAQAGLETVARSYDLLVYPL
                     PGNLDALMQQVSAGHPVLVMQDRLFGGPGSQFALLVGYDQRERTLVLRSGNTRRWYTS
                     FASFDDAWGEAGRWAVLVMPTNQLPAQPLKQTWLTAAQALQQQGRAAAAQRALRTAHQ
                     VWPDAQL"
     gene            complement(11137..12420)
                     /locus_tag="VFF22_07115"
     CDS             complement(11137..12420)
                     /locus_tag="VFF22_07115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZX16618.1"
                     /translation="MAHTAYPQSYYAASANPVPARPELNGEVETDVCIVGAGYTGLST
                     AIALLENGFKVSIVEAAKVGFGASGRNGGQIVNSYSRDIDVIERSVAPKQAQLLGDMA
                     FEGGRIIRERIAKYNIQCDLKDGGVFAANTPKHMKHLESQKKLWERYGHTQLELMDTK
                     RIREVVGTDIYAGGMLDMSGGHIHPLNLALGEAAAVESLGGVIYEQSPAIRIERGANP
                     VVHTPNGRIKAKFVVVAGNAYLGNLIPELSAKSMPCGTQVITTEPLSAELAKSLLPQD
                     YCVEDCNYLLDYYRLTSDKRLIFGGGVVYGARDPANIEAIIRPKMLKAFPQLKDVKID
                     YAWTGNFLLTLSRLPQVGRLGDNIYYSQGCSGHGVTYTHLAGKVLAEALRGQAERFDA
                     FADLPHYPFPGGRLFQVPFSALGAWYYTLRDKLGV"
     gene            12607..12683
                     /locus_tag="VFF22_07120"
     tRNA            12607..12683
                     /locus_tag="VFF22_07120"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12641..12643,aa:Val,seq:gac)
BASE COUNT         2474 a         3420 c         4098 g         2705 t
ORIGIN      
        1 gggacgagcc gcatggatgc ggtgagaggc ttaaagggcc atggacggcc cttgtaagcc
       61 ggcccccgga gcagcgccgg agcgagggaa gttgagcgaa gcgaaacccg gatgtcgggt
      121 gtgctttctc tttggttact ttctctgtcg cacgagcata cggagcgaag cggagtaaca
      181 gccgcaggct ggcccgaagg gcgagcgaag cgagtaaaga gaaagtaact cgccgtaagg
      241 gcgaaaccgg tagaaacagg cagcgaagaa aataaatcca ccccgatccc ctattagttt
      301 tttacttctt attgctgata aggcctgcta atgattccat tattttctgg ttgcttgctt
      361 ggtcagattt atatttctca aggtcaactg ttgactcgcc tttaccaagc ttgaagtttc
      421 tctcggttga aactagctcg gtgatcactt gggcaatgga ctcttcattg tttgccataa
      481 cagccttttc tgctgcgata aatttcagtt ccgcattagt gagttcgttc tgaaaatatt
      541 taatttcacc aagtccagtt ttgtaaagct ttagaaagaa aaatgaaaaa atttctatga
      601 atatgcttag agttagtctt ggggcgtaat aggcggctag ctcatttgaa gttagtgtgt
      661 ggtttccaga taaaacagta gtggcgagta cggtgacagc tgaaattgtg gtgataacac
      721 caattactaa atttaagttc cctcgtcggc ctaaggcttc tatttctctt gttagtcttg
      781 ctcttacttg ttggcattgt tctcttaggt cggatatgta tttatctttt cggatgtcag
      841 ttgagtattt ctcttcaatc gcagttaaga agctttcgga agcattctta agaatatcgt
      901 tttttatatt tgagatgatc tcgatttttt cctcatcact taatagctcg taatttctat
      961 ctctagttga gattttttct tttagagcct gtacttcttc aaatagactc tcgctatggc
     1021 ggctgaaatt tgagttgttt tttaactctt tcctcaggat agagagctct tcacgcatta
     1081 attcttcatt ggtatttctt ttttcgccaa aaatctgtcc tgattttaga tagttaagaa
     1141 gaaatacaga tacagacatc atgataagtg cgactggaat ggccagtatt atgcctggtg
     1201 atgaagtttc atatccgtcg aatatgtctt tgatgagtat aaatattatc cctgttacgc
     1261 cagccaatat tgctgtggca ttcataaatc tggattgttt aattctgctt tctatttcca
     1321 ttgtgtgctc cttgatttct tatatgcgcc atgactgtca tgcgggctac taactagacg
     1381 acaccactat cgcaatctgt tgctgcatgt gccgcattaa tggaaagggg atggatttat
     1441 ttttaaggtg aaggggcaga ggccaagtca tcaaatggca actagctagt cttatggtcc
     1501 cgaccataag cactcgcgtt gcaaacaaca accccgccaa ccttaccctt cctgcgccct
     1561 ataagccccc ggcgttaacc cggtccattt cttaaacgcc cgatgaaacg ccgacggttc
     1621 ggaaaacccc agctgctcgg caatatcctg aatcgccaac tcatcgcgcc ccaggtggta
     1681 aatcgccagg tcgcggcgca gatggtcttt caattcctga aagctcgagc cttcctcgcg
     1741 caagtgccgg cgcagggttt gtgggctcat atgcagttgc tgggcaacgg catccagatc
     1801 cggccagttg ctgcagtcgc ggccgagcag gcggcggatt tggctgatca ggctgtcgcc
     1861 gccatccggg cgggccagca ggtcggcggg ggagtgctgg aggaattgct tgagggtgcg
     1921 ctcatcctgc agcagcggca tggccaggta gcgcgcatgg aatagcaggc tggtgcgccc
     1981 ggccgtgaag cgccgggtgc aggggaatag cagctcgtat tcgccggcgt gggcgggttg
     2041 gctgtaggtg aaggtggctt cttccaggcg gatgcgttgg ccgatcagcc agctgccgag
     2101 gcgatgccag atcaccagca ggctttccac cagaaagtgg tcggggtccc acagggtcga
     2161 gtcgtccacg cttagccgtg cccagtcgcc ttcacgctgg atgctgatgg ctggggcttc
     2221 ggggaacagg ctgtagaaca gcgcgccacg gcccagggct ttttccagcg aatggcagtg
     2281 gatgatggcg taacacatca tggcgaaggt gccgcgcttg ctgcgcaggc ggccgaagcc
     2341 gaggtattcg tcgtcgaggc tttcccacaa aagttgcacc aggcgggcga actgttcggg
     2401 cgcgacccgg gcgcggggct cgtcgagcag ggcgggttgg atgcccagtt ggcgcagcaa
     2461 cggtgtgcag tcgtggccct ggcgctcggc accacgcagg gcggcgcgga caaaatggct
     2521 ggcgatggtg cgttcgcgca tgggctgggc tcggaaaagg gctcggcgat cttagccagg
     2581 gtcgggtgcc gacaacaagc cgaagccggt gttggctatc cgccttagtg ggggcgcttg
     2641 gcgcagcaag tgcgatgggc tgccctggtg cgcacagcgc acctacgtat tgtcggcagc
     2701 tccgttgggc agcggccagg gcaggtgcgc ttcgataaag gcttgcagtt gttcggccgg
     2761 cagggggcgg gcgtagaggt aaccctgcac ttcgaaacaa cccagctcaa acagggcatg
     2821 ggccgtggcg ttgtcttcga cgccttcggc tatggtcgcc aggcccaggg agcgggccat
     2881 ctgcacgatg gcggtgacga tcgcgcggct atcgctgctg ccattgaggt cgcggacaaa
     2941 ggactggtcg atcttcagct tgtccaccgc cagatggcgc aggtaggcca ggctggaata
     3001 gccggtgccg aagtcgtcga tggacagccg cacgcccatg gccttgaggc gcttgagggt
     3061 gtcgagtacc tgatcggtct tgtcgagcag gctcgactcg gtgatttcca gctcgagcat
     3121 ctgcgcggat agctgcgagc tggccagggc ctgtagcacc agttgctcga ccttgccctg
     3181 ttgcaattgg atggccgaga tattcactgc cacctgcgca atgctcagcc cggcgcgatg
     3241 ccaggcggca gcctgtttgc atgcctggtg caggatccat tcgcccatgg gcacgatcag
     3301 ccggctctct tcggcggcgg ggataaagcg cccaggcgga agcaggccca gttcggggtg
     3361 ctgccaacgc accagggcct cgacgccgat cagcgcgccg ctgcgcaggt cgatctgcgg
     3421 ctggtagtgc agcagcaact catggttgtc gatggcctgg cgcaggccgt tgagtagggt
     3481 gaggtgttcc agggcttcgt cgttcatcgc cgcatcgaag aagcgccagg tctgccggcc
     3541 atcctgtttg gccttgtaca tggcggtttc ggcgttgcgc aacaggcttt cgaaactgtt
     3601 gccgtcgtgg ggggcgaggg cgacgccaag ggacagcgag gtgttgatgc tgcggccgag
     3661 gatgctgaag ggctgtcgca cctgctgcat gatctgctgg gccatgtcgg cggcgtcgtc
     3721 ggcatgctcc agttcgcgca gcagcatgat gaactcgtcg ccgccggagc ggcacagggt
     3781 gtcgccgccc ttgagcagcg gtcgcaggcg ctcggccagg ccacgcagta gggcatcgcc
     3841 ataggagtgg ccgagggagt cgttgagggt cttgaaaccg tcgacatcca taaatatcag
     3901 ggcgacacat tcggcactgc cgtgttgggc gaccagggcc tggtcggtgc ggtcgcgcag
     3961 caggtcgaga ttgggcaggc cggtcaggct gtcgtagaag gccagcacct cgatcttctg
     4021 ctccgcggcc tggcgctcgg ccatttcggc ggccaggcgg cgatagggtt catgcacgct
     4081 ggaaacgaac accacgcggt agataaacca gtaggacagc accttgtaca ggtggccgag
     4141 caggctgtag atgtcgttga cattgctgta ggcggtaaag cacagctcgg acagtagcga
     4201 cagggccacg gcgagaaaca ggccggcggc atcgtatggc tgcgggctca ggctgtcgcg
     4261 ccagaacaac agcgccgcca gggcgaacag ggtaatgatc agccactcca ggcggacctt
     4321 cagggtggtc aggccctggc catcgataaa catctgaggg aaccagcccg ggtgccagag
     4381 ttgcaggtag gtgaacagcg cgctcagcag cacggccgcg gccagcagca ggtagcggct
     4441 acggctgcgg cgcagcggcg tccaggggcg cagggccacg gtgagcaggc aaagggcggc
     4501 cactaggcgc gccgccagcc agaagtcgat cgccttgttc gggctggccg gggtgatgaa
     4561 accgggcatg ccgcgatagg acagaacatg ggcgaagtcc agaaaaccca cggcgagaaa
     4621 gccgcaggcg aggatcagca ggttgccggc acgctcggcg cggtagctgt gccaggtgac
     4681 gctgaacacc agcatggcca ccacgatggc gaagaattca gtgctggtat gcagccaggt
     4741 gggaaaccag ccgtggctca gtttcagctc ggaggacgag ggcaatagcc acaccagtag
     4801 gagcactagc gccaggctgc tgagcagggt gtagccgaaa cggtttggca gggcggcgag
     4861 ggggcggatg gcgggaaagg tatcgggcat caaggtctgc cggtgcggaa ggtagaggtc
     4921 gccggggagg ctgcgcggcg cgctaattat ccatgatttt tacagggtta taacgactat
     4981 agccaaggac tgcgatgctg cctaagtcgt agaccgtggc ggattgggtc aatccaggct
     5041 gagcgcatgg gtcattgagg cggcgggggc gtggggccta acctgctgca cgacttcagc
     5101 tcagggagca cagccgcatg cagcgcctta ttttcgacac cgaacacaac atgttccgcg
     5161 acgcttttgc cgcctttctg aaaaaggaag tggtgccgca tcaggacgaa tgggaagcgg
     5221 ccggcgtggt cagccgtgag gtttggaaga aggccggcga catgggcttt ttgctgccgt
     5281 gggcggatga ggagtacggc ggcgccgggc tcaaggattt tcgctacgag cagatcatgt
     5341 gcgaggagct ggcgaagatt aacgagccgg gctttatgct gccgctgcac tcggcgctgt
     5401 gcgggcccta tatcgccgag tacggcaatg ctgagcagaa agcacgcttt atgccgggga
     5461 ttatcagcgg cgagctgatt ctggcggtgg cgatgaccga gccgagcgcc ggttccgacc
     5521 tggccggcat gcgcaccagt gcggtggatc agggtgatca ttacgtactc aacggttcca
     5581 aggtgtttat ctccaacggt ctgctggccg atgtggtgat cgtcgcggcc aagactgatc
     5641 cggccaacaa acacgccatg ggcctgttcc tggttgagcg cgggatggac ggcttcgagc
     5701 gcggcaaaaa cctggaaaaa ctcggtatga agagccaaga caccgccgag ctgttcttca
     5761 ataacgtcaa ggtgcccaag gccaacctgc tgggcgacgc taagggcggc ttcttctacc
     5821 tgatgaatat gctcgcccag gaacgcctaa ccaacgcctg cggcgcggtg gcggcagccg
     5881 aggcggcgtt gcaggtgacc atcgattatg tgcgcgaacg caaagccttc gaccggccga
     5941 tttcgcactt ccagaacacc cgtttcaagc tggcggaaat gcgcacgcag atcgacgtgg
     6001 cgcaggtgtt tgtcgaccgc tgcgtaaccg accacaacga gaagaagctc accccggaag
     6061 tggccgccga ggccaagctg ttcaccaccg agttgctgtg caaggtggtc gacgaaggtg
     6121 tgcaactgca cggcggctgg ggttatatgt gggagtaccc gatctgcaag atgtacgcga
     6181 acgcgcgcat ccagcgcatc ttcgccggca cctcggagat tatgaaagag atcattagcc
     6241 gtgggatgaa gctctgagca tcagggtttt aatactgtag gagcctgctt gctggcgaag
     6301 ctttctcgat gatcggatcg ccagcaagct ggctcctaca aagtgcaatg ttcaatggac
     6361 caggcgttat ccgagcgagt acgaggttgt tgttcaagcg ctaacgttca tctcggcaaa
     6421 acaataagaa cgagggattg cgatgacgga tcaactgccc ctggagcgcc tggcgctctg
     6481 ggttgagaaa cgccccgatg cggtctggct gagccagccg gtaaacggcc agtggcatga
     6541 cttcacctgg gcgcaggtgg acgaccaggc gcggcgcatg gccacggcct tgcacgcgct
     6601 gggttgcgca ccgggtgatc gggtggcgct gctggcgaag aactgcgccg agtggatcat
     6661 tgctgacctg gcgatcatgc tcgccgggct ggtcagcgtg ccgttgtatc cgttgcagtc
     6721 ggcggaaagc atcgactacg tgctgcgtca ttcgcagtgc aaggcgatct tcctcggcaa
     6781 gctggatgag ccggccaaac tggcgccggg cattccggcc gacgttatgc gtatcgccat
     6841 gccgtatcca accatcgagg cccagcacaa ctggcaggaa ctgctggcgg tgcatcagcc
     6901 gttacgcgat ggccatttgc aggcgccgga tgagttgctg agcatcctct atacctccgg
     6961 caccaccggc cagcccaagg gcgtgatgct ctcggccaag gcctttgcca ttgccggcgg
     7021 gcgctcggtg aaggaactgc agattaccga gcaggatcag tatttctcct acctgccgct
     7081 gtcgcatgcc gccgagcgtt ttctggtgga aatgaatgcc ctgtacagcg gtgggcgggt
     7141 ggcctttgtc gagtcgcttg agacctttgc cagcgacctg cgccatgtac ggccgaccgt
     7201 gttcttctcc gtgccgcgcc tgtggacgcg ctttcagcag ggcgtgctgg agaaactgcc
     7261 gcagcccaag ctggcgcggt tgctggggat tccgctgatc ggcggcctgg tggcacgcaa
     7321 gattcgcaag gggctgggcc tggatcgcgc gcgcattctg gtcagcggcg cggcggcgat
     7381 tccgcgtgcg ttgctcgact ggtaccagag catcggcatc accatctgcg agggctacgg
     7441 catgaccgag cacctggcct atggctgttt caatcggccg gggcaggtgc gttttggcac
     7501 ggtgggccgg ccgatgccag gtaatgaatt gcgcatcgcc gagaatggcg aaattctcct
     7561 gcgctgcccg agcctgatgc tgggctacta ccaggacccg gagaaaactg cggaaaccat
     7621 taccgatggc tggctgcata ctggtgacaa gggcgaggtg gatgcggccg gttatttgaa
     7681 aatcaccggg cgggtgaagg acatcttcaa aaccagcaag ggcaagtaca tcgccccggc
     7741 acctattgaa ggggagatcg ccaaaaacct ctgggtcgag caggtatgcc tgatgggcag
     7801 caatctggat cagccactgg cgttgatcga actgtcgcca gcggctcgcg agcatgcccg
     7861 tgaacaggtg gcggccgacc ttgcgcagac gttgcagcag cttaatgccc agcttgaggc
     7921 ccacgagcgg cttagccact ttgttttggt gcgcgaggcc tggaccgtgg acaacggctg
     7981 catgaccccg accatgaaga tccgccgcaa cgtactggaa gcgcgctttg ccgaccagct
     8041 agacgagctg gatgccaagc agccgctgca ttggcagtga ggcgtgtgct tttgtaggag
     8101 ctagcttgct tgcgatcatt accgtgcaaa agcatcgcca gcgagctggc tcctacagag
     8161 attgatttat tcagcaccgc aggatgggtc gggccgcgca ggttgagcgc agcgataccc
     8221 atcggcatta acgattatcc aaaggagctg ttatgtcagg cccgctgtcg tcactgaaaa
     8281 tgctggattt ctccaccctg ttgcccgggc cgtttgcctc gttgctgctg gccgatatgg
     8341 gcgcggaagt gctgcggatt gagtcacctt cgcgcatgga cctggtgcgc gtgctgccgc
     8401 cgcaagatgg cggcgtatcg gccagccacg cctatctcaa ccgcaacaag cgctgtatcg
     8461 ccctggacct gaaacaggcc gaggcggtgg cgatggttaa acagttggtg gccgagtacg
     8521 acatcgtgct ggagcagttt cgccctgggg tgatggacaa gctcggtgtc ggttatgaag
     8581 cgctcaaggc gatcaacccg aagctgattt atgtatcgat cactggctac ggccagagcg
     8641 gcccgtacaa ggaccgcgcc ggccatgaca tcaactacct ggccttggcc ggtatcgcca
     8701 gttacaccgg acggcgtgaa accgggccgt tgccgctggg cattcaggcg gcggatattg
     8761 ccggtggttc actgcacggg gtgattggtt tgctagcagc ggtaatccag cgccaggcca
     8821 cggggcaggg tcagcaggtg gatatcagca tgaccgattg cgccttcagc ctgcacggca
     8881 tggccggcgc gggttatctg gcggccggtg tggagccggc gatggaaaac caggcgctga
     8941 atggcggcag tttctacgac tactaccgca cccgcgacgg ccgctggttc tcggtcggta
     9001 gcctggagcc gcagtttatg cagcagttct gcgccgccat cggccgcccg gagctggccg
     9061 cacgcggcct gtcgcagaag cccgaagatc agcaggcgct taaacgcgag atcgaaatcg
     9121 agttcgagaa acgcgatttc gccgagtggg gccaggtgtt cgccgaactg gatgcctgcg
     9181 tggagcccat gctcaacctc agcgaagcgg tcgagcaccc acagatcaag gcccgtggcc
     9241 tggtcaccga ggtgccgcgt gcaggtaatg gcccgcaggc gcagatcgcc tgcccgatca
     9301 aattctccag cggcttgcct gcgccccggc atatcggagc ggtactcggc gcgcacaccg
     9361 ctgaagtact ggcggaactg ggctacagcg ccgagcagat cgcccagctc aaggcagcca
     9421 aggtggtggc ctgatccagc gtttgcgttt tgcgggtcaa agatactcgt caaatagctg
     9481 attttccggc ataagcctgg ggcattcgag ccagctgact gttcgggaac gaaaaatatg
     9541 tatcacgggg aacgcttcaa cgcctggacc catctggtcg gggcaattct ggcctgcgca
     9601 ggcgcggttt ggctcttggt gctggcaagc ctggatggcg agttgcgcaa gatcatcagc
     9661 gtggcgatct atggcctgac cctggtgttg ctctacagca tctccacgct ctaccacagc
     9721 gtgcaggggc gggcgaaggt ggtgatgcgc aagctcgatc acctgtcgat ctacctgctg
     9781 attgccggca gctatacgcc gttctgcctg gtgaccctcg aaggtgcctg gggctggacg
     9841 ttgtttggca tcgtctggac gctggccgtg atcggcatgt tgcaggagat caagccgcgc
     9901 tccgaggcgc gggtgctttc gattgtgatc tacgcggtga tgggctggat tgtgctaatt
     9961 gcggtgaaac cgctgatcgc tgcgctgggc atgacgggtt ttatctggct ggccggcggc
    10021 ggggtgctgt acacggtggg catcatcttc ttcgcctatg acagccgctt ccgccattgg
    10081 catggcatat ggcacctgtt cgtcatgggc ggcagcctga tgcactttgt tgccgtgctg
    10141 cgttacgtcg tgtaattcgg cgcggcctta gggttgatca atttgatgcc ctatagccta
    10201 cggtgcctgg tgagtcagtg tgggcgcaat gcccggcaat taatcagcca gaagagggtt
    10261 ttcgtttgtg cgcatggctt ggctgctgcg tgcaggctag ttcggtattt tgcgcggatg
    10321 ggttgggtca ccggcacttc atcgttatgc tctgcgccac aaacaagcca gcgggtgagt
    10381 gatgtttcag tctgtgcgcg tcgtaaagct tctgccgatt gccctgttgt ttcttcttag
    10441 tgcctgcacc agcgggccgc agctgctgcc tgagaccaag cgtttgcctg agcgggtcga
    10501 gctggccgat gtgccgttct ttcaactcag tgatgccccg ggcggacctt cggcgttggc
    10561 ggcgctgctc aatcattatg gggtgatcac cagccctggc ttggtggatg agcgcattca
    10621 gcaactggcc aaagggcaaa gcgcgcaagc cgggcttgag acggtggcgc gctcttatga
    10681 tctgctggtc tatcccttgc ccggtaatct ggatgcgctg atgcaacagg tgtcggcagg
    10741 gcatccggtg ctggtgatgc aggatcgtct gtttggcggg ccgggttcgc agtttgcgct
    10801 gctggtcggt tatgaccagc gtgagcgcac gctggtactg cgttcaggca atacccggcg
    10861 ttggtacacc agttttgcca gcttcgatga tgcctggggc gaggcggggc gttgggcggt
    10921 gttggtcatg cctaccaatc aattgccggc gcagccgctc aaacagacgt ggttgacggc
    10981 agctcaggcc ttgcagcagc aaggtcgtgc ggcggcagcg cagcgggcct tgcgtacggc
    11041 acatcaggtt tggccagatg cacagctgta aaacgttgaa aggtagaatg caaaacggca
    11101 cctcttgagg tgccgttttt gtatgcgtgc tgtgctttag acgcccaact tatcacgcag
    11161 ggtgtagtac caagcaccca gggcactgaa tggcacttgg aataggcgcc cgcctgggaa
    11221 ggggtagtgc ggcaggtcgg cgaaggcgtc gaagcgttcg gcctggccgc gcagggcttc
    11281 ggccagcact ttaccggcca ggtgggtgta ggtcacgccg tggccgctgc agccctgtga
    11341 gtagtagatg ttgtcgccga ggcggccgac ttgcggcagg cgcgacaggg tcagcaggaa
    11401 gttgccggtc caggcgtagt cgatcttcac atccttcaac tgcgggaagg ccttgagcat
    11461 cttcggtcga atgatcgctt cgatgttcgc cgggtcgcgt gcgccgtaga ccacgccgcc
    11521 gccgaagatc aggcgcttat cactggtcag gcggtagtag tcgagcaggt agttgcagtc
    11581 ctcgacgcag tagtcctgtg gcagcaggct tttcgccagc tcagcggata gaggctcggt
    11641 agtgattacc tgggtgccgc acggcatcga cttggccgac agttctggaa tcaggttgcc
    11701 caggtaggcg ttgccggcga cgaccacgaa cttggcttta attcggccat taggggtatg
    11761 cacaacagga ttagcaccac gctcgatacg gatggcaggc gattgctcgt aaatcacgcc
    11821 gcccagagat tcgactgctg cggcttcacc cagggccagg ttgagcgggt ggatatggcc
    11881 tccgctcata tcgagcatgc cgccggcgta aatgtcggtg cccaccactt cacggatgcg
    11941 cttggtatcc atcagttcca gttgggtgtg gccgtagcgt tcccacagct ttttttgcga
    12001 ctccaagtgc ttcatatgct tgggggtgtt ggcggcgaat acgccaccgt ccttaaggtc
    12061 gcactggatg ttgtacttgg cgatgcgctc gcgaatgatc cggccgcctt cgaaggccat
    12121 gtcgcccagc agttgcgcct gcttgggggc gacgctgcgt tcgatcacat cgatatcacg
    12181 gctgtagctg ttaacgatct gtccgccatt acggcccgat gcgccgaaac caaccttggc
    12241 cgcttcgacg atgctgactt taaagccgtt ttcgagcagg gcgatggccg tggacaggcc
    12301 ggtgtagccc gcgccgacga tgcacacgtc agtctcgact tcgccattca actccggacg
    12361 cgccggtacc gggttagcgg aggccgcgta ataggattgt ggataagctg tgtgcgccat
    12421 tttgcaacct ctgttcttta ttttttacga atgggcggat gctacctgag ttaaaaaagc
    12481 tcggctagtg gcctgtgcga aatattaaac gcgctgtaga acagtaaaaa attcagttat
    12541 tcaggcagtt agcgtgaaaa ataggttgac agttttttgc gcggccgtag aatgcgcacc
    12601 catcgtaggc acgtagctca gttggttaga gcaccacctt gacatggtgg gggtcgttgg
    12661 ttcgagtcca atcgtgccta ccagatttgg tagaaaa
//
LOCUS       DASRUM010000107        17502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2228549, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000107 DASRUM010000000
VERSION     DASRUM010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 17502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17502
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2228549"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..1226
                     /gene="tsaD"
                     /locus_tag="VFF22_07125"
     CDS             201..1226
                     /gene="tsaD"
                     /locus_tag="VFF22_07125"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZX16619.1"
                     /translation="MLVLGLETSCDETGVALYDSERGLLADALFSQIDLHRVYGGVVP
                     ELASRDHVKRMLPLIRQVLSEADCVATDIDAIAYTAGPGLVGALLVGASCAQALAFAW
                     DIPALGVHHMEGHLLAPMLEEQPPAFPFVALLVSGGHTQLVRVDGIGQYQLLGESLDD
                     AAGEAFDKTAKLMGLQYPGGPEIARLALSGTPGRFKFPRPMTDRPGLEFSFSGLKTFT
                     LNTWQQCQAAGDKSEQTRCDIALAFQQAVVDTLTIKCKRALKQTGLNNLVIAGGVSAN
                     QALRQSLEKMLAELKGGVFYARPAFCTDNGAMIAYAGCQRLLAGQHEDLSIKVQARWP
                     MEQLPAL"
     gene            complement(1262..1831)
                     /gene="plsY"
                     /locus_tag="VFF22_07130"
     CDS             complement(1262..1831)
                     /gene="plsY"
                     /locus_tag="VFF22_07130"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HZX16620.1"
                     /translation="MFWLLATLAYLLGSLSFAILLSRLSGGPDPRASGSGNPGATNMF
                     RVAGKRLAILTLIGDLLKGLLPVLIASLLDFSLQQQAWVGLAAVVGHLYPLYFNFHGG
                     KGVATAAGMLLGLYPPAALLALTAWALTFYLTRTSSLASLVATPLTLPLLAWQQPDAL
                     LPVCVLTGLIVWRHRGNLRDLLAGRERHF"
     gene            1906..2262
                     /gene="folB"
                     /locus_tag="VFF22_07135"
     CDS             1906..2262
                     /gene="folB"
                     /locus_tag="VFF22_07135"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HZX16621.1"
                     /translation="MDTVFIEGLEVDTVIGAYDWERTIRQCLRLDLYLGWDNRPAAAG
                     DELDKALDYAKVSQRIQAFASESQFILVETFAERLVELLMTEFQIPWVRLKLTKPGAV
                     PAASGGVGVEIERGCR"
     gene            2253..2789
                     /gene="folK"
                     /locus_tag="VFF22_07140"
     CDS             2253..2789
                     /gene="folK"
                     /locus_tag="VFF22_07140"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HZX16622.1"
                     /translation="MSLTPVLLGLGSNIGREAHLCAGLDALAGLLSEMRCSPVFESQA
                     VGIKSGPFFNLVVAGYSELPLGELDRRLKFIEADNGRYAPERKGLPLDIDVLLYGDLV
                     GNFDGLILPRAEILKNAFVLWPLSLLVPDVLHPGEQRRFTELWQAAQIDQALWPVAFS
                     WREQQLTPSELIQAYPAS"
     gene            complement(2786..4024)
                     /locus_tag="VFF22_07145"
     CDS             complement(2786..4024)
                     /locus_tag="VFF22_07145"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019693930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HZX16623.1"
                     /translation="MQIYKVGGAVRDRLLGRAVSEIDWVVVGASAEQMLEQGYRPVGA
                     DFPVFLHPQSGEEYALARTERKSGRGYGGFTFFASPEVTLEEDLIRRDLTVNAMAEDD
                     HGTLIDPYGGQRDLEARLLRHVSPAFAEDPLRVLRVARFAARYAPLGFSVAPETMTLM
                     RELSESGELSALMPERSWKEISRALMEPRPDVFIQVLRDCGALRELLPEVDALFGVPQ
                     PAAHHPEIDTGEHVLSVLRQCAEHQQPLTVRWACLLHDVGKGLTPETEWPRHIAHEHT
                     GLKLIRAINQRCKAPKDCQELALQVGEFHTHGHRALELKASTLLKLLQGFDVFRRPQR
                     FEEFIAACEMDARGRLGLEQRAYPQADYLRAAMHTARAVAVQPLLAQGLKGAELGEAL
                     NRERLQALTAYKASAAPQSG"
     gene            complement(4131..4643)
                     /locus_tag="VFF22_07150"
     CDS             complement(4131..4643)
                     /locus_tag="VFF22_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HZX16624.1"
                     /translation="MRLFIVLLLCLALATCSSQLTRVQVLTPASTELTGKRSFSLIAA
                     EQAVEGDVPFAERYAQLEQLLREGLSARGYQANPQADIQVYYWLAVQDSPLDFKVDDA
                     PPNPLGPYQAIHRLRDETGTLRIRLTTPEQQILWEGLISTGLSPAKDSAELLERATQA
                     LLQQIPMASP"
     gene            complement(4706..6274)
                     /locus_tag="VFF22_07155"
     CDS             complement(4706..6274)
                     /locus_tag="VFF22_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017739510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HZX16625.1"
                     /translation="MSNSKDKRRQPISTGSEWTFELIRQYDREIGRLAERYALDTYPN
                     QIEVITAEQMMDAYASVGMPLGYHHWSYGKHFLSTEKGYKRGQMGLAYEIVINSDPCI
                     AYLMEENTICMQALVIAHACYGHNSFFKGNYLFRTWTDASSIIDYLVFAKQYIMQCEE
                     RHGIDAVEDLLDSCHALMNYGVDRYKRPYPISAEEERRRMKDREEHLQKQINDLWRTI
                     PKNASKGGDKDNKRFPAEPQENILYFLEKHAPLLEPWQREIIRIVRKIAQYFYPQRQT
                     QVMNEGWATFWHYTLMNDLYDEGLVTEGFMMEFLQSHTSVVYQPPFDSPYYSGINPYA
                     LGFAMYRDIRRICEEPTEEDRRWFPEIAGSDWLSTLKFAMTSFKDESFILQYLSPKVI
                     RDLKLFSILDDDQKDELYVPAIHDEPGYQQIRETLAAQYNLGNREPNVQIWNIDRRGD
                     RSLTLRHQQHDRKPLGNSTEEVLKHLHRLWGFNIHLELYQGEQLIGTQHVPPKHEAEH
                     DSDYPRLDLMIPPI"
     gene            complement(6271..7542)
                     /locus_tag="VFF22_07160"
     CDS             complement(6271..7542)
                     /locus_tag="VFF22_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019693928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HZX16626.1"
                     /translation="MSHVIDRRLNGKNKSTVNRQRFLRRYRDHIKKAVEEAVSRRSIT
                     DMEHGEQISIPGRDIDEPVLHHGRGGRQTTVHPGNREFTTGEKIARPQGGGGGQGAGK
                     ASNSGEGMDEFVFQITQEEFLDFMFEDLELPNLIKRHLTGSDTFKSVRAGISNEGNPS
                     RINIVRTLRSAHARRIALSGSSRNKLKSTQAELERLKLEQPDNLGDIQALEEEVSKLR
                     ARIKRIPFLDTFDLKYNLLVKQPNPSSKAVMFCLMDVSGSMTQSTKDIAKRFFILLYL
                     FLKRNYDKIDVVFIRHHTSAKEVDEEEFFYSRETGGTIVSSALKLMQEIMTERYPINE
                     WNIYAAQASDGDNWNDDSPICRDILINQIMPFVQYFTYVEITPREHQALWFEYEQVSE
                     AFADTFAQQQLVSAGDIYPVFRDLFQRRLAT"
     gene            complement(7642..9564)
                     /locus_tag="VFF22_07165"
     CDS             complement(7642..9564)
                     /locus_tag="VFF22_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HZX16627.1"
                     /translation="MSIFSHFQQRFEATRQEELTLQEYLELCKQDRSAYASAAERLLL
                     AIGEPELLDTSTDPRLSRIFSNKVIRRYPAFADFHGMEECIDQIVSYFRHAAQGLEEK
                     KQILYLLGPVGGGKSSLAEKLKHLIEKVPFYAIKGSPVFESPLGLFSDAEDGAILEED
                     YGIPRRYLNSIMSPWATKRLQEFGGDISQFRVVKLYPSILNQIAVAKTEPGDENNQDI
                     SALVGKVDIRKLEEYPQNDADAYSYSGALCRANQGLMEFVEMFKAPIKVLHPLLTATQ
                     EGNYNSTEGLGAIPYSGILLAHSNESEWHSFRNNKNNEAFIDRIYIVKVPYCLRVTDE
                     IKIYDKLLFNSSLAKAHCAPDTLKMLAQFSTLSRLKEPENSNVYSKMRVYDGENLKDT
                     DPKAKSIQEYRDTAGVDEGMNGLSTRFAFKILSKVFNFDPHEIAANPVHLLYVLEQQI
                     EQEQFPAEVRERYLRFIKEYLAPRYIEFIGKEIQTAYLESYSEYGQNIFDRYVLYADF
                     WIQDQEYRDPETGEILNRVALNEELEKIEKPAGISNPKDFRNEIVNFVLRARANNNGK
                     NPTWLSYEKLRVVIEKKMFSNTEDLLPVISFNAKASKEDQQKHNDFVTRMVERGYTDK
                     QVRLLSEWYLRVRKSQ"
     gene            complement(9840..10169)
                     /gene="glpE"
                     /locus_tag="VFF22_07170"
     CDS             complement(9840..10169)
                     /gene="glpE"
                     /locus_tag="VFF22_07170"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011063830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HZX16628.1"
                     /translation="MSEFKRIPPEQAQALRQQGAVVVDIRDPQSFATGHISGSQHLDN
                     HSLHAFITQADLDAPLIVACYHGNSSQSAAAYLVGQGFTDVYSLDGGFELWRATFPDD
                     TAQASEE"
     gene            complement(10166..10987)
                     /locus_tag="VFF22_07175"
     CDS             complement(10166..10987)
                     /locus_tag="VFF22_07175"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HZX16629.1"
                     /translation="MATYAVGDLQGCLKPLQCLLERVAFDPAQDKLWLVGDLVNRGPE
                     SLATLRFLYSIRDALTCVLGNHDLHLLAVAHNIERLKKGDTLREIIDAPDAADLLDWL
                     RRQKLLHYDEARDTALVHAGIPPQWTMSKALKRAAEVEEALRDDARMPLFLDGMYGNE
                     PAKWDGDLHGVTRLRVITNYFTRMRFCKADGTLDLKSKEGVGSAPPGYAPWFSYKQRK
                     TRGQRIIFGHWAALEGQSTEPDVVALDTGCVWGASMTLMNIDSGEKYSCDCKEQA"
     gene            complement(10987..11367)
                     /gene="apaG"
                     /locus_tag="VFF22_07180"
     CDS             complement(10987..11367)
                     /gene="apaG"
                     /locus_tag="VFF22_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005768068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HZX16630.1"
                     /translation="MSDSRYNIDVSVVTRYLPEQSQPEQNRFAFAYTVTVSNKGELPA
                     KLLSRHWVITDGDGRVQEVRGAGVVGQQPLIEAGASHTYSSGTVMTTRVGIMQGSYQM
                     LADDGKRFDALIAPFRLAVPGSLH"
     gene            complement(11409..12212)
                     /gene="rsmA"
                     /locus_tag="VFF22_07185"
     CDS             complement(11409..12212)
                     /gene="rsmA"
                     /locus_tag="VFF22_07185"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZX16631.1"
                     /translation="MSEYQHRARKRFGQNFLHDAGVIHRILRAIHAREGEHMLEIGPG
                     QGALTEGLLNSGAQLDVIELDRDLIPILQHQFGSNPHFRLNQGDALKFDFNQLQAAPH
                     SLRVVGNLPYNISTPLIFHLLSNAALIRDMHFMLQKEVVERLAAEPGGGDWGRLSIMV
                     QYHCRVEHLFNVGPGAFNPPPKVDSAIVRLVPHDVLPHPAKDHHLLERVVREAFNQRR
                     KTLRNTLKNLLPAAAIEAAGVDGSLRPEQLDLAAFVRLADKLAEQPNAD"
     gene            complement(12321..13322)
                     /gene="pdxA"
                     /locus_tag="VFF22_07190"
     CDS             complement(12321..13322)
                     /gene="pdxA"
                     /locus_tag="VFF22_07190"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HZX16632.1"
                     /translation="MTTPRLFALTPGEPAGIGPDLCLLLAREAQPHALIAIASRDLLA
                     ERARLLGLNTQLINVVPDCWPSQAAPAGSLYVWDTPLAAAVTPGQLDASNAPYVLETL
                     TRAGQGCLSGHFAGMITAPVHKGVINEGGIAFSGHTEFLADLTHTEQVVMMLATHGLR
                     VALVTTHLPLKDVAAAITPERLSRVTRILDHDLRTKFGIAQPRILVCGLNPHAGEGGH
                     LGMEEIEIIEPTLQQLRSEGIDLIGPLPADTLFTPKHLEHCDAVLAMYHDQGLPVLKY
                     KGFGAAVNVTLGLPIIRTSVDHGTALDLAGSSKIDSGSLQVALQTAYEMAASQVKSA"
     gene            complement(13458..14750)
                     /locus_tag="VFF22_07195"
     CDS             complement(13458..14750)
                     /locus_tag="VFF22_07195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZX16633.1"
                     /translation="MKIKLSDCVRPLLLGALFLGTAAHAEVQPLNRVVAIVDNDVVMQ
                     SQLDSRLREVQQTIAQRGAALPPEHVLSQQVLERLIIENIQLQIGERSGIRITDEELN
                     QAIGTIAQRNGMSIEQFRAALAADGLSYTDARDQVRREMIISRVRQRRVAERIQVTDQ
                     EVQNFLASDMGKMQLSEEFRLANILIPVPEGASPEDIQAAERQARDLYQQLQQGADFA
                     QLAIARSASETALEGGEMGWRKAGQLPPPFDTMLGAMSVGQVTEPMRTPGGFIMLKVL
                     EKRGGESQVRDEVNVRHILIKPSEIRSEAETKRLVERLYQRILAGEDFAELAKNFSED
                     PGSALNGGTLSWIDPNVLVPEFREVMNNTPAGELSKPFKSPYGWHVLEVMGRRATDSS
                     VQFREQQAMTVLRNRKYDEELQSWLRQIRDEAYVESKL"
     gene            complement(14731..>17502)
                     /locus_tag="VFF22_07200"
     CDS             complement(14731..>17502)
                     /locus_tag="VFF22_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HZX16634.1"
                     /translation="VKYPAFRKKFPLMVTGSLLALQPLAVPFAIAAEQYDCQVSATGG
                     WACAPKTSNAALPARPVQRDSVASSNAGEGSTEAPQGKAAQATLVTESRGRGLASRSA
                     DYSHLDWVPRENLSAAQLAEAGPYCAGAYIEPTRPGMNDQTPMSEAPMFVSAKASRYE
                     QEEQVATLAGDVVLRQAGMQVEADEANLRQAENRGELIGNVRLRDQGMLVVGDRAELQ
                     LGNGEAKVDNAEYVLHKSHIRGNALYAKREESAIIRLKDGTYTSCEPGSNAWHLKGNN
                     ITLNPATGFGTATNVTLRVKNIPVFYTPYIYFPIDDRRQSGFLPPSISSSSDNGLTLQ
                     TPYYFNLAPNYDATLYPTYMADRGLLMEGEFRYLTKNSEGQFGAAYLDDSNDDRKLMS
                     EYEDQRWMYSWQHKTGLDSRLLAEVDYTDISDPYYFQDLDSDLGIDSTSYVDQRGTLT
                     YRGNGYRAQLNAHAYELANITDVTPYNRLPQLTLDGKLPFEPAGLNFTYGTEYVRFDR
                     NLRNGFFTDEDGNTGTNEFRWYDDRLRGLARANGERLHVEPAVSLPLNWSWGFLTPKV
                     KYLHTNYDLSLDSQGKNSLLAEQSYKSSQSRGVGMFSLDSGLYFDRDTQWFGKNYRQT
                     LEPRLFYLNVPEEDQTDIPVFDSSESTFNYASLFRENRFSGKDRIGDENKLSLGVSNR
                     WIESNGFERQRFSIGQAFYFEDRKVQMPGIDYRTRPDAQTSQSPYALQYMYRFNRDWR
                     LSSDFNWDPDTHRTRSGSAMFHYQPEDNPNKVINAGYRYKNDTMRYDRDSATWTNNPD
                     YGTEGSPNYIKDYYKISQHDFSVIWPIMPQWSVISRWQYDYGRDRTLEAFAGFEYDSC
                     CWKLRLINRYWIDYDEVSLNPSLNDQADRGIFLQIVLKGLGGVVGNKVETFLDQGIQG
                     YREREDQAF"
BASE COUNT         3051 a         4859 c         5723 g         3869 t
ORIGIN      
        1 ggcttacctt ctttattacc gggggtaacg ttactcctgg cagatggcgc tggagaacgt
       61 aggtttttaa gggttgcgga tgttaccgcc tcatcgcgag gaatgcaaag cctctgatcg
      121 aaaagcgctg gccggctcgc gccagcggcc attatcatgc gcgccttcga agttgcacac
      181 actactacaa ggcactgccc atgctggttc tgggattgga aacctcctgc gacgaaaccg
      241 gtgtcgcgct ttacgacagt gaacgtggcc tgctggccga tgcgctgttt agccagattg
      301 acctgcaccg tgtgtatggc ggtgtggtgc cggagctggc ctcgcgcgat cacgtcaagc
      361 gtatgttgcc gttgatccgc caggtgttga gcgaggctga ctgcgtggcg actgacatcg
      421 acgccatcgc ctatacggcg ggtcccggcc tggtcggtgc gttgctggtt ggcgcttcct
      481 gcgcgcaggc cctggcgttt gcctgggata ttccggcgct gggtgtgcac cacatggaag
      541 gccacttgct ggcgcctatg ttggaggaac aaccgccagc ctttccgttc gtcgctttgt
      601 tggtgtcggg cgggcatacc cagctggttc gcgtcgatgg catcggccaa taccagttgc
      661 tcggcgagtc tttggatgat gccgcaggcg aggccttcga caagaccgcc aagctgatgg
      721 ggctgcagta cccaggtggg ccggagattg cccgactggc gctgagcggt acgcctgggc
      781 gcttcaagtt cccccggccg atgactgatc gcccggggtt ggagttcagc ttcagcggcc
      841 tgaagacctt taccctgaat acctggcagc agtgccaggc ggctggcgac aaatccgaac
      901 agacccgttg cgatatcgcc ctggcgttcc agcaggcggt ggtcgatacc ctcaccatca
      961 aatgcaagcg tgcgctcaag cagaccgggt tgaacaatct ggtgatcgcc ggcggcgtga
     1021 gtgctaacca ggcgctgcgt cagtcgctgg agaagatgct cgctgagctg aagggcggcg
     1081 tgttctacgc ccgcccggcg ttctgcaccg ataacggcgc gatgatcgcc tatgccggct
     1141 gccagcgttt gctggcgggg cagcatgaag atttgagcat caaggtgcag gcgcgttggc
     1201 cgatggagca attgccagcg ctttaagagc ctgcgggatg agccggtttc actcggtcta
     1261 tttagaaatg ccgctcacgg ccggcgagca gatcgcgtaa attgccgcga tggcgccaga
     1321 cgatcagccc tgtcagcacg cagacgggca gcagagcatc gggttgttgc caggccagca
     1381 ggggcagggt cagaggtgtg gccaccaggg atgccagtga gctggtacgg gtcaggtaga
     1441 aggtcagggc ccaggccgta agggccagca gcgcggcggg gggatacaga cccagcaaca
     1501 tgccggcggc cgtggcgaca cctttgcccc catggaaatt gaagtacagc ggatacaggt
     1561 ggccgacaac ggctgccagg ccgacccagg cttgctgctg caggctgaag tcgagcaggc
     1621 tggcgatcag tactggcagc aggcctttga gcaagtcgcc gatcagcgtg aggatggcca
     1681 ggcgtttgcc ggcgacccgg aacatgttgg tggccccggg attgcccgag ccactggcgc
     1741 gcgggtctgg tccgccgctt aggcggctga gcaggatggc gaaggacaac gagccgagca
     1801 ggtaggcgag ggtcgccaac agccaaaaca tggtatccat tccggggcaa gggagccctg
     1861 attctaacta ggtgcgccag tattgtcgtg ccgcggagag cgtgcttgga cacagttttt
     1921 atcgagggtc tggaagtcga tacggtgatt ggtgcctacg actgggaacg caccattcgc
     1981 cagtgtttac gcctggatct gtacctgggc tgggataacc gtcctgctgc tgctggtgat
     2041 gagctggata aggccttgga ttacgccaag gtctcgcagc ggattcaggc gtttgccagc
     2101 gagtcgcaat ttatcctggt ggaaaccttt gctgagcggc tggtcgagtt actcatgact
     2161 gagtttcaga ttccctgggt gcgactcaag ctgaccaagc ccggtgcggt accggctgcc
     2221 agtggtgggg ttggcgtgga gatcgagcgc ggatgtcgct aactcccgtt ctacttggcc
     2281 tgggcagtaa tatcggccgc gaagcgcacc tgtgcgccgg cctcgatgcg ctcgccggct
     2341 tgctcagtga gatgcgctgc tcaccggtat tcgaaagcca ggcggtgggc atcaagagcg
     2401 gcccgttctt caacctggtg gtagccggtt acagcgagtt gccgttgggt gagctggacc
     2461 gtcggctgaa gtttatcgag gccgataacg gccgctatgc gccggagcgt aaaggcctgc
     2521 cgttggatat cgacgtgctg ctgtatggcg atctggtcgg caactttgac ggtctgattc
     2581 tgccgcgggc agaaatcctc aagaacgcct ttgtgctctg gccgttgtcc ttgctggtgc
     2641 cggatgtatt acatccgggc gagcagcgcc gctttaccga gctttggcag gcggcgcaga
     2701 ttgaccaggc gctgtggccg gtggcattca gctggcgcga acagcagctg acgccgtcgg
     2761 agttgattca ggcatatcct gcctcttagc ccgattgcgg ggccgcgctt gctttgtatg
     2821 ccgtgagggc ctgcagccgt tcgcggttta acgcctcccc cagttccgca cccttgaggc
     2881 cctgcgccag cagcggttgc acggctactg cgcgggcggt gtgcatggcg gcgcgtaggt
     2941 agtcggcctg gggataggcg cgctgttcca ggccgaggcg gccgcgggcg tccatttcac
     3001 aggcggcgat aaattcttcg aagcgctgcg gacggcggaa cacgtcgaag ccttgcagca
     3061 gcttgagcag ggttgaggct ttcagctcca gggcgcggtg gccgtgggtg tggaattcgc
     3121 cgacctgcag agccagttct tggcaatcct tgggcgcctt gcagcgctgg ttgatcgcgc
     3181 ggatcagctt cagtccggtg tgttcgtggg caatatgccg gggccattca gtctcgggtg
     3241 tcaggccttt gccgacatcg tgcagcaggc aggcccagcg cacggttaga ggttgctggt
     3301 gttcggcgca ctggcgcagc acgctaagta catgttcgcc ggtatcaatt tccgggtgat
     3361 gggcggcggg ctgcggcaca ccgaacagtg cgtcgacctc cggcaacaac tctcgcagcg
     3421 cgccgcagtc gcgcagcacc tggataaata catccgggcg tggctccatc agcgcacggg
     3481 aaatttcctt ccagctacgt tctggcatta gggcgcttag ctcgccggac tcgctcagtt
     3541 cgcgcatcaa cgtcatggtt tccggcgcta cgctgaagcc cagtggcgcg tagcgggcgg
     3601 caaagcgggc gacgcgcagc acgcgtagcg ggtcttcggc gaaggctggg gatacgtggc
     3661 gcagcaaccg ggcttcgagg tcgcgttggc cgccgtaggg gtcgatcagt gtgccgtgat
     3721 cgtcttcggc catggcgttg acggtcaggt cgcggcggat caggtcttct tccagggtga
     3781 cttccgggct ggcgaagaag gtaaagccgc cgtagccgcg cccactttta cgctcagtgc
     3841 gggccagggc gtattcctcg ccgctctgcg ggtgcagaaa gaccgggaaa tctgcaccta
     3901 ccggacgata gccctgttcg agcatctgct cggcgctggc gccgactacg acccagtcga
     3961 tctcgctgac cgcgcggccc aacaggcgat cacgcactgc gccgcctact ttgtaaatct
     4021 gcatcatggt tcctccacta agtgcggagg atagcggctg gtcgttgggg aggggtagct
     4081 cggatggcag ttctgagggt ttttcagttt gtccctggat tgcactcggg ctacgggctg
     4141 gccataggga tttgctgcaa tagcgcctgg gtggcgcgtt ccagcagttc ggcgctgtct
     4201 ttggcggggc ttaggccagt gctgatcaga ccttcccaga ggatttgctg ttccggggtg
     4261 gtcaggcgga tgcgcagggt gccggtttca tcgcgcaggc ggtggatcgc ttggtagggg
     4321 ccgagtgggt ttggtggagc gtcgtcgacc ttgaagtcca gtgggctatc ctgcaccgcc
     4381 agccagtaat acacctgaat atctgcctgc ggattggcct ggtaaccgcg cgcgctcaaa
     4441 ccttcgcgca acaattgctc aagctgtgca taacgctcgg caaatggtac gtcgccttct
     4501 acggcctgtt cggcggcgat caggctgaaa ctgcgtttgc cagtcagttc ggtgctggcg
     4561 ggtgtcagca cttggacgcg cgtcagctgg ctgctgcagg tggctagggc caggcacagc
     4621 agtaacacga taaacaggcg catcggcaga ctcccggttg gtctgctaag catgctggta
     4681 cttggcatcg tgggcgcgca accgatcaaa tgggcgggat catcaggtcg aggcgcgggt
     4741 agtcgctgtc atgttcggcc tcatgcttgg gtggcacatg ctgggtgccg atcagctgtt
     4801 cgccctggta cagctccagg tggatattga agccccacag gcggtgcagg tgtttcagca
     4861 cttcctcggt cgagttgccc agcggtttgc gatcatgctg ctgatggcgc agggtcagcg
     4921 agcggtcgcc acggcgatcg atgttccaga tctgtacgtt gggctcgcgg ttgcccaggt
     4981 tgtattgcgc ggccagggtt tcacgaattt gctgataacc gggctcgtcg tggatcgccg
     5041 gtacatacaa ctcgtctttc tggtcgtcat cgagaatgct aaacagcttg aggtcgcgga
     5101 ttaccttggg cgacaggtac tgcaggataa aactctcgtc cttgaagctg gtcatggcga
     5161 atttcagggt gcttagccag tcactgccgg cgatctccgg gaaccagcgg cgatcttctt
     5221 cggtgggctc ttcgcagatg cggcggatgt cgcggtacat ggcaaagccc agggcatagg
     5281 ggttgatgcc gctgtagtag gggctgtcga acggcggctg gtagaccacg ctggtgtgcg
     5341 actgcaggaa ctccatcata aagccttcgg tcaccaggcc ttcgtcgtac aggtcgttca
     5401 tcagggtgta gtgccagaag gtggcccagc cttcgttcat cacctgggtc tggcgctgcg
     5461 ggtagaagta ctgggcgatc ttgcgcacga tgcggatgat ctcgcgctgc cagggctcca
     5521 gcagcggtgc gtgtttctcc aagaagtaga ggatgttttc ctgcggttcg gcggggaagc
     5581 gtttgttgtc cttgtcgccg cccttgctgg cgtttttcgg gatggtgcgc cagaggtcgt
     5641 tgatctgctt ttgcaggtgc tcttcgcggt ccttcatgcg ccgccgttct tcttcggcgg
     5701 aaatcgggta agggcgcttg tagcggtcga cgccgtagtt catcagggcg tggcaggagt
     5761 cgagcaagtc ctccaccgcg tcaatgccgt ggcgctcctc gcactgcatg atgtactgct
     5821 tggcaaacac caggtaatcg atgatcgagc tggcatcggt ccaggtgcgg aacaggtagt
     5881 tgcccttgaa gaagctgtta tggccgtagc aggcatgggc gatcaccagg gcttgcatgc
     5941 agatggtgtt ctcttccatc aggtaggcga tgcacgggtc ggagttgatc acgatttcgt
     6001 aggccaggcc catctgcccg cgcttgtagc ccttttcggt gctgaggaag tgtttgccgt
     6061 aggaccagtg gtggtagccc aacggcatgc cgaccgaggc gtaggcgtcc atcatctgct
     6121 cggcagtgat cacctcaatc tgattggggt aagtgtccag ggcatagcgt tcggcgagtc
     6181 tgccgatttc gcggtcgtac tggcggatca gctcgaaggt ccattccgag ccggtagaga
     6241 tcggttggcg gcgcttgtct ttgctgttgc tcatgtggct agcctccgct ggaacaggtc
     6301 gcggaatacc gggtagatgt cgccggccga gaccagctgc tgctgggcga aggtgtcggc
     6361 gaaggcttcg ctgacctgct cgtattcgaa ccacagcgcc tgatgctcgc gcggggtgat
     6421 ctcgacgtag gtgaagtact gcacgaacgg catgatctgg ttgatcaata tatccctgca
     6481 gatcggcgag tcatcattcc agttgtcgcc atccgacgcc tgggcggcgt agatattcca
     6541 ttcgttgatc gggtaacgct cggtcatgat ctcctgcatc agcttgagcg cgctggagac
     6601 gatggtgccg ccggtttctc gtgaatagaa gaactcctcc tcatccactt ccttggcgct
     6661 ggtgtggtgg cggataaaca ccacgtcgat cttgtcgtag ttccgcttga ggaacaggta
     6721 caggaggata aagaagcgtt tggcgatgtc cttggtcgac tgggtcatgg agccggaaac
     6781 gtccatcagg cagaacatca ccgccttgga gctggggttg ggctgtttga ccagcaggtt
     6841 gtacttgagg tcgaaggtgt cgaggaaggg gatgcgcttg atccgcgcac gcagtttgct
     6901 gacttcctct tccagtgcct ggatatcgcc gaggttgtcc ggctgttcca gtttcaggcg
     6961 ttccagctct gcctgggtgc tcttgagctt gttgcggctg ctgccggaca gggcgatgcg
     7021 ccgggcgtgg gccgaacgca gggtgcggac gatgtttata cgcgacgggt tgccttcatt
     7081 gctgatgccg gcgcgcacgc tcttgaaggt gtcgctgccg gtcaggtggc gtttgatcag
     7141 gttgggtagc tccaagtcct cgaacataaa gtcgagaaac tcttcttggg ttatctggaa
     7201 gacaaactca tccatgcctt cgccggagtt gctcgccttg ccagcgccct gaccgccgcc
     7261 accgccttgc gggcgggcaa tcttctcgcc tgtggtgaac tcgcggttgc cggggtgcac
     7321 ggtggtctgg cggccgccac ggccgtggtg cagcaccggt tcgtcgatat cacggccggg
     7381 gatgctgatc tgttcgccgt gctccatatc agtgatggag cggcgactga ccgcctcttc
     7441 cacggccttc ttgatgtggt cgcggtaacg ccgcaggaag cgctggcggt ttaccgtgct
     7501 cttgttcttg ccgttcaggc gtcggtcgat cacatggctc ataactgccc tccgtgcgct
     7561 gcagacatca ggcgcagcca gcacagagcg gcgtttttcg cacgccctgc acccgctggc
     7621 agcctgttgc gtcaggccgc gttattgcga tttacgaacc cgcagatacc actcggacag
     7681 caggcgtacc tgtttgtcgg tgtaaccgcg ctcgaccatg cgagtgacga agtcgttgtg
     7741 tttctgttga tcctccttgc tcgctttggc gttgaagctg atgaccggca gcaggtcttc
     7801 ggtgttggag aacatcttct tctcgatgac cacgcgcagt ttttcgtagc tgagccaggt
     7861 cgggttcttg ccattgttgt tggcgcgagc gcgcagcacg aagttgacga tctcgttgcg
     7921 gaaatccttc ggattgctga tcccggccgg tttttcgatc ttctccagtt cctcgttaag
     7981 cgctacgcgg ttgagaatct cgccggtttc cgggtcgcgg tattcctgat cctgaatcca
     8041 gaagtcggcg tacagcacat agcggtcgaa gatgttctgc ccgtattcgc tgtaggactc
     8101 caggtaggcg gtctggattt ccttgccgat aaactcgata tagcgcggcg ccaggtactc
     8161 cttgataaag cgcaggtaac gctcgcgtac ctcggcaggg aactgttcct gctcgatctg
     8221 ctgctccagc acgtagagca ggtgcaccgg gttggcggcg atctcgtggg ggtcgaagtt
     8281 gaacaccttg gagaggatct tgaaggcgaa gcgggtcgag aggccgttca tgccttcatc
     8341 cacgccagcc gtgtcgcggt actcctgaat cgacttggcc ttggggtcgg tgtctttgag
     8401 gttttcgcca tcgtacacgc gcatcttcga gtagacgttg gagttttccg gctctttcag
     8461 gcggctgagg gtggagaact gcgcaagcat cttcagcgtg tctggcgcgc agtgagcctt
     8521 ggccagggag ctgttgaaca gcagtttgtc gtagatcttg atttcgtcgg tcacgcgcag
     8581 gcagtacggc accttgacga tataaattcg gtcgataaag gcctcgttgt tcttgttgtt
     8641 gcggaagctg tgccattccg actcgttgga gtgggccagc aggatgccgc tgtaggggat
     8701 cgcgccgagg ccttcggtgc tgttgtagtt gccttcctgg gtcgcggtga gcagtggatg
     8761 cagcaccttg atcggtgcct tgaacatctc gacgaactcc atcaggccct ggttggcccg
     8821 gcacagtgcg cccgagtagc tgtaagcgtc ggcgtcgttc tgcgggtatt cctcaagctt
     8881 gcggatatcc accttgccga ccagggcgga gatgtcctgg ttattctcat cacccggttc
     8941 ggtcttggcc acggcgatct gattgagaat cgacgggtag agtttgacca cgcggaactg
     9001 gctgatatcg ccgccaaact cctgcaggcg cttggtggcc cagggcgaca tgatgctgtt
     9061 gaggtagcgc cgtgggatgc cgtaatcctc ttcgagaatc gcgccgtcct cggcgtcgct
     9121 gaacagtccc agtggcgact cgaatacggg cgagccctta atcgcataga acggcacttt
     9181 ctcgatcagg tgcttgagct tctctgccag ggaggattta ccgccgccca ccggcccgag
     9241 caggtagagg atctgtttct tttcttccag accctgcgcc gcatggcgga agtaggagac
     9301 gatctggtcg atgcactctt ccatgccgtg gaaatcggca aaggccggat accggcggat
     9361 caccttgttg gaaaaaatcc gcgacagcct tgggtcggtc gaggtgtcga gcagttccgg
     9421 ctcgccaatg gccagcaaca ggcgctcggc tgccgaggca taggcgctgc gatcctgttt
     9481 gcacagttcg aggtactctt ggagggtgag ttcctcctgg cgagtcgctt cgaaacgttg
     9541 ttggaagtgg ctgaaaatgc tcatgacttc acctcgctcg atgcgcagag ccggtgcggg
     9601 atcggtcagg tgcttggcag ctccggtgcg gctgcagccg ggataaaacc cccagacacc
     9661 taatagtctt accgattgcc cgatgccgtt tggccggctc taccttgttt tggatggcct
     9721 gagctaaagg atagttcgga atcagcgggg tcaaggggcg cggaacgata acaggatatt
     9781 accgttggtc gggagggggc ggcaggccgc tgctggtgcg gcctgggcgt tagaaaaaat
     9841 tattcttcgg aggcttgtgc ggtgtcgtcg gggaaggtcg cacgccacag ctcgaaaccg
     9901 ccatcgaggc tgtagacatc agtgaagcct tggccgacca ggtaagccgc cgcgctctgg
     9961 ctggagttgc cgtggtagca ggcgacaatc agcggtgcat cgagatcggc ttgggtgata
    10021 aaggcgtgca gggagtggtt atcgaggtgc tgtgagccgc tgatatggcc ggtggcaaaa
    10081 ctctgcggat cgcggatgtc gaccactact gcaccttgct ggcgtaaggc ctgagcctgc
    10141 tcggggggga tgcgtttgaa ttcgctcatg cttgctcctt acagtcgcag ctgtatttct
    10201 cgccgctatc gatgttcatc agggtcatgc tcgcgcccca gacacagccg gtatccagag
    10261 cgaccacgtc gggctccgtg gattgccctt ccagcgccgc ccagtggccg aagatgatcc
    10321 gttggccacg ggtcttgcgc tgtttgtagc tgaaccaggg ggcgtagccc ggtggtgcgc
    10381 tgccgacacc ttccttgctt ttcaggtcga gggtgccatc ggctttgcag aaacgcatgc
    10441 gggtgaagta gttggtgatc acccgcaggc gcgtgacgcc gtgcaggtcg ccgtcccact
    10501 tggctggctc attgccatac atgccgtcga gaaacagcgg catgcgcgcg tcgtcacgca
    10561 gggcttcttc cacttcggcg gcgcgcttga gcgctttgct catggtccac tgcggcggta
    10621 tgccggcatg taccagggcg gtatcgcggg cttcatcgta atgcagaagt ttctgccggc
    10681 ggagccagtc gagcaggtcg gcggcatccg gtgcgtcgat gatctcgcgc agggtgtcgc
    10741 ctttcttcag tcgttcgata ttgtgcgcca cggccagcag gtgcaggtcg tgattgccca
    10801 gcacgcaggt cagcgcatcg cggatgctgt agagaaagcg cagcgtcgcc aaggactcgg
    10861 ggccgcgatt gaccaagtct ccgaccagcc acagtttatc ttgggccgga tcaaaggcca
    10921 cgcgctcaag caggcattgc aggggtttca ggcagccttg taggtcgccg accgcgtaag
    10981 tggccatcag tgcaacgagc ccggcaccgc aagacggaag ggcgcgatca gggcgtcaaa
    11041 gcgtttaccg tcgtcggcaa gcatctgata gctgccctgc atgatgccga cgcgggtggt
    11101 catcactgtg ccgctgctat aggtgtggct ggcgcccgct tcaatcagcg gttgttggcc
    11161 taccacgccg gcgccgcgca cttcctgcac gcggccatca ccgtcggtaa tcacccaatg
    11221 tcgcgagagc agtttggccg gcagctcgcc cttgttgctc accgtgacgg tgtaggcgaa
    11281 ggcaaaacgg ttctgctccg gctgcgattg ctccggtaga tagcgggtga cgacgctgac
    11341 gtcgatgttg taacgcgaat cgctcatgcg taattaactc ggtaggcgga agaccagtct
    11401 agtcgctgtc agtcggcgtt gggttgttcg gccagtttgt ccgccaggcg tacaaacgca
    11461 gcaaggtcca gttgttccgg acgcaggctg ccatcgaccc cggcggcttc gattgccgct
    11521 gcgggtagta ggttcttcag ggtattgcgc agtgtcttgc gccgttggtt gaaggcttca
    11581 cgcaccacgc gctcaagcag gtgatggtcc ttggccggat gcggcagcac atcgtgcggt
    11641 accaggcgga cgatcgccga gtcgacttta ggtggcgggt tgaacgcgcc ggggccgaca
    11701 ttgaacagat gctccacgcg gcagtggtat tgcaccatga tcgacaggcg gccccagtct
    11761 ccgccaccag gctcggcggc caggcgctcg accacttcct tctgcagcat aaagtgcatg
    11821 tcgcggatca gcgcggcatt gctcagtagg tggaagatca gcggcgtgga gatgttgtat
    11881 ggcaggttgc cgaccacgcg caggctatgt ggcgcggcct gcagctggtt gaaatcgaac
    11941 ttgagggcgt caccctgatt caggcgaaaa tgcgggttgc tgccgaactg gtgttgcagg
    12001 atcgggatca gatcgcggtc gagttcaatc acgtcgagct gcgcgccgct gttcagcagg
    12061 ccttcggtga gcgcgccctg gcctggcccg atttccagca tgtgctcgcc ttcgcgggcg
    12121 tggatggcgc gcagaatgcg gtgaatcacc ccggcgtcat gcaggaagtt ctggccgaaa
    12181 cgcttgcgcg cgcggtgttg gtattcggac atgcagcagc tccaggcttc aaagcatcgg
    12241 cttgaagcga atgtaacgcg ggtagaaagc ccggcagttt aacagcattg gtggtgtccg
    12301 ccccgtgctg ggggtgacag tcaggcgctt ttcacctggc tggcggccat ttcatacgcg
    12361 gtttgtaggg cgacctgcag gctgccgctg tcgattttgc tgctgccggc cagatccagc
    12421 gctgtgccat ggtcgaccga ggtgcggatg atcggcaggc ccagggtgac gttcaccgct
    12481 gcgccaaagc ctttgtattt gagcaccggc aagccctggt cgtggtacat cgccagcacc
    12541 gcatcgcaat gctcaaggtg cttgggggtg aacagggtgt cggctggtag cgggccgatc
    12601 aggtcgatac cttcgctgcg cagctgctgc agggtgggct cgatgatctc tatttcctcc
    12661 atgcccaggt gaccgccttc acccgcatgc ggattgaggc cacagaccag aatgcgtggc
    12721 tgggcgatgc cgaatttcgt gcgcaggtcg tgatcgagaa tgcgcgtgac gcgactcagc
    12781 cgctcaggag taatggcggc ggctacatcc ttgagcggta agtgcgtagt gacaagtgct
    12841 acgcgcagac cgtgtgtggc aagcatcatc accacttgct cggtatgcgt cagatcggcg
    12901 agaaactcgg tgtgtccaga gaaggcaata ccgccttcgt tgatcacgcc cttgtgcacg
    12961 ggtgcggtaa tcatcccggc gaagtgcccg gagaggcagc cctgtccggc gcgagtcagg
    13021 gtttctagca catagggcgc gttgctcgca tccagctggc cgggcgttac ggcggcagcg
    13081 agtggcgtgt cccagacata taggctgcct gccggcgctg cctggcttgg ccagcagtca
    13141 gggacgacgt taatcagttg agtgtttagc cccagcaatc gggcgcgctc agccagcagg
    13201 tcacggctgg cgatggcaat cagggcgtgt ggctgcgctt cgcgcgccag cagcaggcac
    13261 aggtcggggc ctataccggc aggctctcca ggggtgagtg caaaaagacg tggcgtggtc
    13321 atcggcgggc tctcaggggc tatgcgccaa ctctgttgcg gcggctgcgg ttagtaaagg
    13381 ggcgagcggt ttgcaggcga aaaaaagccc ggctctgcat atgcataaag ccgggcttga
    13441 tcagtcgcgc tgacctgtca gagcttgctc tcgacgtagg cttcgtcgcg gatctgacgc
    13501 agccaggact gcagttcttc atcgtatttg cggttacgca gtacagtcat cgcctgctgc
    13561 tcgcggaact gcacactact gtccgttgca cggcggccca tgacttccag aacatgccag
    13621 ccgtaagggc tcttgaatgg tttggacagc tcgccagctg gcgtgttgtt catcacttcg
    13681 cggaattctg gcaccaatac gttcgggtca atccagctca gggtaccgcc attaagtgcg
    13741 gagcctgggt cttcagagaa gtttttcgcc agttcggcga agtcttcgcc ggccagaatg
    13801 cgctggtata gacgttcgac caggcgcttg gtttcggctt cgctacggat ttcactaggc
    13861 ttgatcagga tgtggcgaac atttacttca tcacgtactt ggctttcacc gccgcgcttt
    13921 tccagcacct tgagcatgat aaagccgccg ggagtacgca tcggctccgt gacttggcca
    13981 accgacatcg cgccgagcat ggtgtcaaag ggcggtggca gctgcccggc tttacgccag
    14041 cccatttcgc cgccttccag cgcggtttca ctggctgagc gggcaatcgc cagctgggca
    14101 aaatcagcgc cctgctgcaa ttgctggtac agatcgcgag cctggcgttc agcggcctgg
    14161 atatcctcag gcgacgcacc ttcgggtact ggaatcagga tgttcgccag gcggaattct
    14221 tccgatagct gcatcttgcc catgtccgag gcgaggaagt tctgtacttc ctgatcggtg
    14281 acttgaatgc gctccgccac gcggcgttgg cgcacacggc tgatgatcat ttcgcggcgc
    14341 acctggtcgc gggcatcggt gtacgacagg ccgtctgccg ccaaggcggc gcggaactgc
    14401 tcgatgctca tgccattgcg ctgggcgatg gtgccgatgg cctggttcag ctcttcgtcg
    14461 gtaatccgaa tgccggagcg ctcaccaatc tgcagctgga tgttctcgat gatcaggcgc
    14521 tccagcacct gttggctgag cacatgttca ggcggcaagg ctgcgccgcg ctgggcaatg
    14581 gtctgctgta cttcacgcag gcgtgaatcg agctggcttt gcatgacgac atcgttatcg
    14641 acaatcgcta caacgcggtt gagtggttgt acctcagcgt gtgccgcagt acccagaaac
    14701 agcgcgccca acagcagggg gcgcacgcaa tcagaaagct tgatcttcac gttcacgata
    14761 accttgaatg ccttggtcga ggaaagtctc aactttattg ccgactacgc cgcctagacc
    14821 tttgagtacg atctgcagga agatgccgcg gtcagcctgg tcgttcagcg acggattcag
    14881 gctgacttcg tcgtagtcga tccagtagcg gttgatcagg cgcagtttcc agcagcaact
    14941 gtcgtactcg aagccggcaa aggcttccag ggtacggtcg cggccatagt catactgcca
    15001 gcgggagatc acgctccact gcggcatgat cggccagata accgagaaat cgtgctgact
    15061 gatcttgtag tagtccttga tgtagttcgg ggagccttcg gtgccatagt ccggattgtt
    15121 ggtccaggtg gcactatcgc ggtcatagcg catggtgtcg ttcttgtaac gatacccggc
    15181 gttgatcacc ttgtttgggt tgtcttcagg ctggtagtgg aacatcgcgc tgcccgagcg
    15241 agtcctgtga gtgtccgggt cccagttgaa gtccgagctc aggcgccagt cgcggttgaa
    15301 gcgatacata tactgcagcg cataaggcga ctgcgaggtc tgtgcgtccg ggcgggtgcg
    15361 gtagtcgatg ccgggcatct gtaccttgcg gtcttcgaag tagaaggcct gcccgatgct
    15421 aaagcgctgc cgctcgaagc cgttgctttc aatccagcgg ttgctcacgc ccagcgacag
    15481 cttgttctcg tcgccgatac ggtccttgcc ggaaaaacgg ttctcgcgga acagcgaggc
    15541 gtagttgaag gtgctttcgc tggagtcgaa cacagggata tcggtctggt cttcctcggg
    15601 aacattgagg tagaacaggc gcggttccag ggtctggcga tagtttttgc cgaaccattg
    15661 ggtgtcgcgg tcgaagtaca ggccgctgtc caggctgaac atgccgacgc cacggctttg
    15721 gctgctcttg tagctctgtt cggccagcag gctgttcttg ccctggctat ccagactcag
    15781 gtcgtagttg gtgtgcaggt atttgacctt gggcgtaaga aagccccagc tccagttcag
    15841 cggcaggctg acagcgggtt caacatgcag gcgctcgcca ttggcgcggg ccaggccgcg
    15901 caggcggtcg tcgtaccagc ggaattcatt tgtgccggtg tttccatctt catcggtgaa
    15961 gaagccgttg cgaaggttgc gatcaaagcg cacatattct gtgccgtacg taaagttcaa
    16021 ccccgccggc tcgaagggca gcttgccgtc cagagtcagc tgcggcagac ggttgtatgg
    16081 cgtgacatcg gtgatattgg ctagttcgta ggcgtgggca ttgagctgcg ccctgtagcc
    16141 gttgccgcga taggtcaggg tgccgcgctg atcgacatag ctggtggagt cgatgccgag
    16201 atcgctgtcc agatcctgga agtaatatgg atcgctgatg tcggtgtaat cgacttcggc
    16261 cagcaggcgc gagtccagac cggttttgtg ctgccagctg tacatccagc gctggtcctc
    16321 atactcggac atgagcttgc ggtcgtcgtt gctgtcgtcg aggtaggccg cgccgaactg
    16381 gccttcgctg ttcttggtga ggtagcggaa ttcgccctcc atcagcaagc cgcgatcagc
    16441 catgtaggtt ggatacaacg tggcatcgta gttcggcgcc aggttgaaat agtaaggtgt
    16501 ttgcagggtc aggccgttgt cgctcgagct gctgatgctc ggcggcagga agccggactg
    16561 gcgacggtca tcaatcggga agtagatata gggtgtgtag aacaccggaa tattcttcac
    16621 ccgcaacgtg acgttggtcg cggtgccgaa accggtggcc gggttcagag tgatgttatt
    16681 gcccttcagg tgccaggcgt tgctgcctgg ttcacagctg gtgtaggtgc cgtctttcag
    16741 gcggataatt gcgctttctt cacgcttggc gtagagcgca ttgccgcgaa tatggctttt
    16801 gtgcagcaca tactcggcgt tatcgacctt ggcttcaccg ttgcccagtt gcagctcggc
    16861 gcggtcaccc accaccaaca tgccctgatc acgtaggcgt acattgccaa tcagctcgcc
    16921 gcggttttcc gcctgacgca ggttggcttc atccgcttcg acttgcatgc cagcctggcg
    16981 caggaccaca tcaccggcga gggtggcgac ttgctcttcc tgttcatagc gcgaggcttt
    17041 ggccgacaca aacatcggcg cctcggacat tggagtctgg tcgttcatgc ctggccgcgt
    17101 cggctctata taagcgcctg cgcagtaggg gccggcttcc gccaactggg cggcgctgag
    17161 attctctcgc ggcacccaat ccagatggct gtagtcggcg ctgcgcgaag cgaggcctct
    17221 gccacggctt tcagtcacca gggtcgcttg cgcggctttc ccctgaggtg cttcggtgct
    17281 gccttcacca gcattgctgc tggcgaccga atcacgttgt actggccgtg ctggcagtgc
    17341 agcattgctg gtcttcgggg cacatgccca gccgccggtg gcggaaacct ggcaatcgta
    17401 ctgctcggca gcgatagcaa aaggaacggc taaaggttgt agcgccagaa ggctgccagt
    17461 gaccattaga gggaattttt tacgaaacgc ggggtatttt ac
//
LOCUS       DASRUM010000108         9885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2394300, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000108 DASRUM010000000
VERSION     DASRUM010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9885
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2394300"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1326
                     /locus_tag="VFF22_07205"
     CDS             136..1326
                     /locus_tag="VFF22_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant NAGGN system M42 family
                     peptidase"
                     /protein_id="HZX16635.1"
                     /translation="MQQLPEPDLVYLQKVLLEMLAIPSPTGFTDTIVRYVAERLEEIG
                     IPFEMTRRGTIRGTLKGKQSSPDRAVSAHLDTIGAIVRELQDNGRLGLAAVGCWSSRF
                     AEGSRVSVFTDHGVIRGSVLPLMASGHAFNTAVDQMPISWDHVELRLDAYCSTRADCE
                     SLGISVGDFVAFDPLPEFTESGHISARHLDDKAGVAALLTALKAIKESGLEPEIDCHP
                     LFTITEEVGSGAAAALPWDVSEFVGIDIAPVAPGQQSSEHTVSVAMQDSGGPYDYHLS
                     RQLLRLASENEIPVRRDLFRYYHSDAQSAVTAGHDIRTALLAFGCDATHGYERTHIDS
                     LKSLSRLLAAYVLSPPVFSSDAQPAQGSLERFSHQLEHDAQMESDTRVPAVDSLLRPR
                     DQDA"
     gene            1407..4160
                     /locus_tag="VFF22_07210"
     CDS             1407..4160
                     /locus_tag="VFF22_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HZX16636.1"
                     /translation="MPRIWLAVVLFWLCGPAWALLPATLDSADLRLSLGPSMAYLEDP
                     QGELSVEQVGALSDERFTPVKGEHANLGKNDSIWWFKVRLNNSLAQNLAGYLEVNYPL
                     LDHLQVFLSTTDGQWLMQESGDRYAFSQRPVQVRNFWFPLELTPGESILLVRVETTST
                     IFMPLFFSTYQASAAAQENLMGFNGAFYGVLFAMFCYNLFLFISLRESAYLWYLAYNL
                     NVGLLAACFDGMLFKLLPEHVAFQSVSIYILMYLHCLTAIQFSRHFLHAPQYFPRLDT
                     GLRLLMLACAGCLLSVPLIGFNAWNILASVTVSLVSLALLLTGLYVWRRGVRYGSYYT
                     LAWAILLFAFIQATTGSLGVEVFGVFGATVVKIGVTIELITLSIGLADRINLLKEEGF
                     QSRRAAEQAEIENQAKSRFLAKMSHEIRTPLNGVLGMLQLLKETPLDRSQRFYVDTIS
                     SSGSSLMAVINDILDYARIESGKLSLEHIEFDLEELISDTLSLFTGQALDKRLRLYVS
                     LESGVPRRAQGDPTRLKQVLMNLLSNALKFTAEGHVALNVCRRNDSQGKPHLVFAISD
                     SGIGISDQALTQLFESFSQGDSSTTRRYGGSGLGLAISKELVEMMGGRIEVQSTPGQG
                     TRFAFDIPQGDDDLPVDPLHELLKGRTALLTSLDGLGLDALSRLLGRWGMRTERCQNP
                     ETLLGYLEDFAAPPLLVLMAPWPGSIQHWLDSLRPLLQPGQRVLLLCAPEQCQQLPSS
                     AGLRLLGLPQPLAINALRSALLELYEEPRAAEIKPPPKTYNDTGSTPCILVAEDNPVN
                     QLVVQGFLKKRGYNVRLVTNGLAALNEYRRDPSATQLILMDCEMPEMDGFEATRQIRR
                     LERQHNLPAVPIIALTAHILDEHRQHGVESGMNDFLGKPLDSAKLYSTLEAYLQPAEP
                     SAQ"
     gene            4229..4468
                     /locus_tag="VFF22_07215"
     CDS             4229..4468
                     /locus_tag="VFF22_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HZX16637.1"
                     /translation="MLIPYHLLEAETLTRLIEDFVTRDGTDNGDETPLDTRIERVRHA
                     LSKGQAVIVFDAESQQCQLALKRDVPKEWLDALEE"
     gene            complement(4572..5000)
                     /locus_tag="VFF22_07220"
     CDS             complement(4572..5000)
                     /locus_tag="VFF22_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16638.1"
                     /translation="MESESIVYGCIRDWPSDSPAESRLRREINRQVLDELPAGDAWSF
                     LGREMFACCEQPGDGLYQTQVIHFGASYRTVEYEWSLWIKQFEALLKRLYWASAVVHL
                     ETELNGTHTFRWESDNGFHSPQEGELRVRCAWEREGGLRG"
     gene            complement(5078..6244)
                     /locus_tag="VFF22_07225"
     CDS             complement(5078..6244)
                     /locus_tag="VFF22_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX16639.1"
                     /translation="MKPFPRAMLLLALILAAINLRPGITSLAPLIERIAEELALSRSL
                     ISLTTALPVLCMGLLAPFAPRLAMRLGLERTIALCMAVIAVALLLRAVGHTSAILIGS
                     AVLLGAAIAVAGPLLSGFIKRHFAGRMGSVVGWYSLSMAIGGAGGAVLTVPATQWLSD
                     DWTYGLAIWALPAVVGLVLWLCLPNRVEAESAEGGLGLPWRQPRAWLISAFFAIQAGL
                     FYALATWAVARYHEAGLSLMHSNSLFSVSMLMGLPSSFLLPWLAQRFKNRYVLLMGCG
                     LLSSISLAMITFQPLVLPELWAVTIGFGLGGSFALSLVLPLYEAGSPLAVSRWTAMML
                     CMGYSIACLTPVLTGLARDLAGNYQLPFMVLTGLAVLMTLLAWMMRRGPKRAHS"
     gene            complement(6374..7180)
                     /locus_tag="VFF22_07230"
     CDS             complement(6374..7180)
                     /locus_tag="VFF22_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX16640.1"
                     /translation="MQNRMMITGAGSGLGREIALRWAREGWQLALSDVNEPGLAETLQ
                     MVREAGGDGFTQRCDVRDYSQLTAFAQACEEKLGGIDVIVNNAGVASGGFFSDLSLED
                     WDWQIAINLMGVVKGCKAFLPLLEKSKGKIVNIASMAALMQGPAMSNYNVAKAGVVAL
                     SESLLIELKLHEVSVHVVCPSFFQTNLLDSFRGPTPAMKAQVGKLLESSPISAADIAD
                     YIYQEIAKGEFMILPHEQGRMAWALKQKNPQLLYNEMTTMADKMRAPRAK"
     gene            complement(7286..7657)
                     /locus_tag="VFF22_07235"
     CDS             complement(7286..7657)
                     /locus_tag="VFF22_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HZX16641.1"
                     /translation="MFWLAVAADAVVVVHLLFILFAVLGGLLVWRWRGLVWLHVPAAL
                     WGVLVELMHWPCPLTPLEHSLRLAAGQAGYSGGFVEHYLLPIIYPAGLTPQIQIGLGL
                     FVLLLNGLIYGVLLWQRWRRP"
     gene            complement(7780..8103)
                     /locus_tag="VFF22_07240"
     CDS             complement(7780..8103)
                     /locus_tag="VFF22_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZX16642.1"
                     /translation="MLKAKLSSLLFAVLTSISLVVVAAETQTQSVPVVETAEVAPVGK
                     VNLNTADAATLQRELLGVGEVKAQAIVAYRDEHGDFASVDELLEVKGIGEATLERNRD
                     KLSTN"
     gene            complement(8476..>9885)
                     /locus_tag="VFF22_07245"
     CDS             complement(8476..>9885)
                     /locus_tag="VFF22_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HZX16643.1"
                     /translation="GIANRSIAGLRVYTSKHIQQMINETGADEILLAMPSASRGRRRQ
                     ILEELEHFPLHVRSIPGIMDLASGRVTVEDVQEVDIADLLGRDSVPPQQVLFERCIRN
                     QVVMVTGAGGSIGAELCRQILLNQPAALLLFEHSEFNLYSIHSELQARIKRESLPLRL
                     VPILGSIRNADRLLDVMQAWKVDTVYHAAAYKHVPMVEHNIAEGVLNNLVGTLNTAQA
                     AIRAGVSNFVLISTDKAVRPTNVMGSTKRLAEMVLQALSRESAPVLFKDDKALRQLNK
                     TRFTMVRFGNVLGSSGSVIPLFHEQIRKGGPVTVTHPNITRYFMTIPEASQLVIQAGA
                     MGQGGDVFVLDMGQPVRIAELAEKMIHLSGLSVRSEKNPHGDISIEFTGLRPGEKLYE
                     ELLIGDNVSPTEHPMIMRANEEHLPWELFKVRLSELLDAVERDDYDRVRQLLRETVSG
                     YKPEGEIVDWIHMKRRLEP"
BASE COUNT         2211 a         3238 c         2622 g         1814 t
ORIGIN      
        1 gccaaggctt attcgggcta cgccgcttat gcccaaacgt agcccggata agcgcagcgc
       61 aatccggggc cccctagcag gcggatcgac gtggtattgc cggccgctcc agcccatctc
      121 acaaggaaac cttgtatgca gcaactcccc gaacccgacc tcgtctatct gcagaaggtg
      181 ctgctggaaa tgctcgccat tcccagcccc accggcttta ccgacaccat cgtgcgctac
      241 gtcgccgagc gcctggagga gatcggtatc cccttcgaga tgacccgtcg cggcactatt
      301 cgcggcaccc tcaaaggcaa gcagagcagc ccggatcgcg cggtctcggc gcacctggac
      361 accattggcg cgatcgtccg cgagctgcag gacaacggcc gccttggcct ggccgcagtc
      421 ggctgctggt cgagccgttt cgccgaaggc agccgcgtca gcgtgtttac cgatcacggc
      481 gtgattcgcg gcagcgtact gccgctgatg gcctcaggac acgccttcaa caccgcagtg
      541 gatcagatgc cgatcagctg ggaccatgtc gaactgcgcc tggatgccta ctgcagcacc
      601 cgcgccgact gcgaatcact gggcatctcg gtgggtgatt ttgtcgcctt cgacccactg
      661 cccgagttca ccgaaagcgg ccatatcagc gcccgccatc tggatgacaa ggccggcgtc
      721 gccgctctgc ttaccgcgct caaggccatc aaggaaagcg gcctggagcc ggaaatcgac
      781 tgccatccgc tgttcaccat cactgaagaa gtcggctcag gcgccgccgc cgcgctgccc
      841 tgggatgtca gcgaatttgt cggcatcgac atcgccccgg ttgccccggg ccagcagtcc
      901 agcgaacaca cggtgagtgt ggccatgcag gattccggcg ggccgtacga ctatcacctg
      961 tcgcgccaac tgctgcgcct ggccagcgaa aatgaaatcc cggtacgtcg agacctgttt
     1021 cgctactacc acagcgacgc acagtctgcg gtcaccgccg gccacgatat ccgcaccgcc
     1081 ctgctggcct tcggctgcga cgccacccac ggctatgaac gcacgcatat cgacagcctc
     1141 aagtctctca gccgtttgct tgccgcttac gtgctcagcc caccggtatt ctccagcgat
     1201 gcccagccgg cgcagggctc gctagagcgc tttagccacc aactggagca cgatgcgcag
     1261 atggaaagtg acacccgcgt gcccgctgtc gacagcctct tgcgcccccg cgatcaggac
     1321 gcttgaaggg ccgcgcgcat ccgggcaaag tgacggcatg acgcggggcc ggatcaggcc
     1381 tcagataata atattgaggt cggtgaatgc ctcggatatg gctggcggtg gtgctgttct
     1441 ggctgtgcgg gccggcatgg gcactgctgc ctgcaacgct ggacagcgcc gacctgcgcc
     1501 tgtcactcgg cccgtccatg gcctatctgg aagacccgca gggcgagctg agcgtcgagc
     1561 aagtcggcgc cctgagcgat gagcgcttta ccccagttaa gggcgagcac gccaacctcg
     1621 gcaagaatga ttcgatctgg tggttcaagg tacgcctgaa caacagcctg gcgcagaatc
     1681 tggccggcta tctggaggtc aactacccgc tgctcgacca tctgcaggtg ttcctgtcca
     1741 ctaccgatgg gcagtggctg atgcaggaaa gcggcgatcg ctacgccttc tcccagcgcc
     1801 cggtacaagt gcgcaacttc tggtttccgc ttgagctaac acccggtgaa agcatcctgc
     1861 tggtgcgggt tgagaccacc agcacgatct ttatgccgct gttcttcagc acctaccagg
     1921 ctagcgccgc cgcccaggaa aaccttatgg gtttcaatgg cgctttctac ggcgtgctgt
     1981 ttgcgatgtt ctgctacaac ctgttcctgt ttatctccct gcgcgaatcc gcctacctct
     2041 ggtacctggc ctacaacctc aatgttggtc tactggccgc ctgtttcgac ggcatgctgt
     2101 tcaagctgct gcccgagcat gtggcgttcc agtcggtgag catctacatc ctgatgtacc
     2161 tgcactgcct gacggccatt cagttcagcc ggcatttcct gcatgcgccg cagtactttc
     2221 cgcgcctgga taccggcctg cgcttactga tgctggcctg cgctggctgc ctgctgtcgg
     2281 tgccgctgat cggcttcaac gcctggaata ttctggccag cgtcaccgtc tcgctggttt
     2341 ccctggcact gctgcttacc ggtctctacg tgtggcgccg cggggtgcgt tacggctcct
     2401 actacaccct ggcctgggcc atcttattgt tcgcctttat ccaagccacc actggctcgc
     2461 tgggggtgga agtctttggg gtgttcggcg ccacggtggt gaagatcggc gtaaccatcg
     2521 agctgattac cctgtccatc ggcctggccg atcgcatcaa cctgctcaag gaagaaggct
     2581 tccagtcgcg ccgtgccgcc gagcaggctg aaatcgaaaa ccaggccaag agccgctttc
     2641 tggccaagat gagccacgag atccgcaccc cgctcaacgg cgtgctgggc atgctgcagc
     2701 tgctcaagga aaccccactg gatcgcagtc agcgcttcta cgtcgacacc atttccagct
     2761 ccggcagctc gctgatggcg gtgatcaacg acatcctcga ttacgcgcgc attgaatccg
     2821 gcaagctcag cctggaacat atcgagttcg atctggaaga actgatttcc gacaccctca
     2881 gcctgttcac cgggcaggcc ctggacaagc gcctgcgcct ctacgtcagc ctggaaagtg
     2941 gcgtaccacg ccgcgcccaa ggcgacccga cgcggctcaa acaagtgctg atgaacctgc
     3001 tcagcaatgc cctgaaattt accgccgaag gtcacgtggc gctgaacgtc tgccggcgca
     3061 atgactccca gggcaaaccg catctggtgt tcgccatcag cgacagcggt atcggcatta
     3121 gcgatcaggc cctgacccaa ctgttcgagt ccttctccca gggcgactcc agtaccactc
     3181 gccgctatgg cggcagcggc ctgggtctgg cgatcagcaa ggagctggtg gaaatgatgg
     3241 gcgggcgtat cgaagtacag agcacgccag gccaggggac gcgcttcgct ttcgatattc
     3301 cgcaagggga tgacgatctg ccagtcgacc cgctgcacga actgctcaag ggtcgtaccg
     3361 cgctgctcac ctccctcgac ggcctcggcc tggatgcatt gagtcgcctg ttaggccgtt
     3421 ggggcatgcg caccgagcgc tgccagaatc cggaaaccct gctcggctat ctggaagact
     3481 tcgccgcccc gccgctgctg gtactgatgg caccctggcc gggcagcatt cagcattggc
     3541 tggactcgtt acgcccgctg ctgcagccag ggcaacgtgt gctgctgctc tgcgcaccgg
     3601 aacaatgcca acaactgccc agttctgccg gcctgcgctt gcttggcctg ccgcagccgc
     3661 tggcgatcaa tgcgctacgc agtgcactgc tggagctcta cgaagaaccg cgcgctgcgg
     3721 aaatcaaacc gccgcctaaa acctacaacg acaccggcag cacgccgtgc atcctggtgg
     3781 ctgaagacaa cccggtgaat cagctggtgg tgcagggctt cttgaagaag cgcggctata
     3841 acgtgcgcct ggtgactaat ggcctggccg ccctcaatga gtaccggcgc gacccgagcg
     3901 ccacgcagct gattctgatg gactgcgaaa tgccagagat ggatggcttc gaagccaccc
     3961 ggcaaatccg ccgtcttgag cgtcagcaca atctgcctgc cgtaccgatt atcgccctga
     4021 ccgcacatat tctcgatgag caccgccagc acggggtcga gtccgggatg aatgacttcc
     4081 tcggcaaacc cctggacagc gccaagctgt acagcaccct ggaagcctac ctgcagccag
     4141 cggagccgtc cgcccagtaa cggcacagca aacatggcca gccagggcca cgcgcgttag
     4201 gatcgcgctg cattctggag accctaccgt gctgattcct tatcacctgc tcgaagctga
     4261 aaccctgacc cgcctgatcg aagacttcgt cacccgcgac ggcaccgaca atggcgacga
     4321 aaccccgctg gatacgcgta tcgaacgggt acgccacgcg ctgagcaaag gccaggcagt
     4381 gattgtcttc gatgcggaaa gccagcaatg ccaattggcg ctgaaacgcg acgtgccgaa
     4441 agagtggctg gatgcacttg aggaataaac gccgcagggt aacctcatcc tcgtaggcta
     4501 gcgctgagcg cagcgaagcc taacaaggcg gcatgttcgt tgggcttcgt cgctgcgcgc
     4561 ctcaacccaa cctacccgcg caggccgcct tcgcgctccc aggcgcagcg cacgcgcaac
     4621 tcaccttcct gcgggctgtg aaagccgtta tccgactccc agcgaaaggt gtgcgtgccg
     4681 ttcagctcgg tttccagatg caccaccgcg ctggcccagt acaggcgctt gagcagcgcc
     4741 tcgaactgct tgatccacag gctccactca tactccaccg tgcggtagct ggcgccgaag
     4801 tggatcacct gcgtctggta cagaccgtct cccggttgct cgcaacaggc aaacatctcc
     4861 cgaccgagaa agctccaggc atcgcccgcc ggcagctcat cgagcacttg ccgattgatt
     4921 tcacgacgca gacggctttc tgccggactg tccgacggcc agtcacggat gcaaccataa
     4981 acgatggatt cggactccac gcaggcactc cagcaaatca aggctgcctt ctatcacagc
     5041 cgtccgggcc aaggaaggaa ctgcagccaa ataagcgtca gctgtgcgcg cgcttcggtc
     5101 cgcggcgcat catccaggcc agcagcgtca tcaacaccgc aagcccggtc agcaccataa
     5161 agggcaactg gtagttaccc gccaaatcgc gcgccaaccc ggtcaacacc ggggtcaggc
     5221 aagcaatgct gtagcccatg cacagcatca tcgccgtcca acggctgacc gccagtggcg
     5281 agcccgcctc gtacaacggc aataccagcg acaaggcaaa agagccgccc agaccaaagc
     5341 cgatagtgac tgcccacaac tcaggcagca ccaacggctg aaaggtaatc atcgccaggc
     5401 tgatcgacga cagcaaaccg cagcccatca acagtacgta gcggttcttg aatcgctgcg
     5461 ccaaccaagg cagcagaaat gaactgggca accccattag catcgacacg ctgaacaggc
     5521 tgttgctgtg catcaggctc aagccggctt cgtgataacg cgccacggcc caggtggcga
     5581 gggcgtagaa caagccggcc tgaatggcaa agaacgcgct gatcaaccag gcgcgcggct
     5641 gcctccaggg caagcccaag ccgccctctg cgctctcggc ctcgacgcgg ttaggcaggc
     5701 acagccagag cacgagaccg actaccgccg gtagtgccca aatggccagg ccgtaggtcc
     5761 aatcatcgct tagccattgg gtggccggca ccgtgagtac cgcaccaccg gcgccaccaa
     5821 tcgccatgct cagcgagtac caacccacca cactgcccat tcgcccggca aagtgccgtt
     5881 tgataaaacc cgacagcaaa ggcccggcaa ccgcgatagc agcgcccagc agcaccgcac
     5941 taccaatcaa aatcgcactg gtatgcccca ccgcacgcag caacagcgcc accgcgatca
     6001 ccgccatgca caaggcgata gtacgctcca gccccagacg catcgccaag cgcggcgcaa
     6061 aaggcgccaa caggcccatg cacaacacgg gcaacgcagt ggtgaggctg atcaggctgc
     6121 ggctcaaggc cagctcttcg gcaatccgct cgatcagcgg cgccagggag gtgatgcctg
     6181 gccgcaagtt gattgcagcc aggatcaggg ccagcaacag catggcccgc gggaaaggtt
     6241 tcacagtaaa tccatcagtt tcgcggccat gccgacatga ccgcaaacct tagagcgcaa
     6301 gctcatcgca gacaaccccg aacggcgatt aagttgaccg ataaatcaac taacccatat
     6361 cggccacgcc ttgtcacttg gcgcgcggtg cacgcatctt gtcggccatg gtggtcattt
     6421 cgttgtacag caactgcggg ttcttctgct tcaacgccca ggccatacga ccctgctcgt
     6481 ggggcagaat cataaattcg cccttggcga tctcctggta aatgtagtcg gcaatatctg
     6541 ccgcactgat cggcgagctt tccagcagct taccaacctg cgccttcatc gctggtgtcg
     6601 ggccgcggaa ggagtccagc aggttagtct ggaagaacga tgggcacacc acatgcacgc
     6661 tgacttcatg cagtttcaac tcgatcagca ggctctccga caacgccaca acaccggcct
     6721 tggccacgtt gtagttgctc atcgccgggc cctgcatcaa ggccgccatg gaagcgatat
     6781 taacgatctt gcctttgctc ttttccagca gcggcaggaa agccttgcag cccttgacca
     6841 cacccatcag gttgatcgca atctgccaat cccaatcctc cagcgataag tcgctgaaga
     6901 agccgccaga ggcaacgcca gcgttgttga cgatcacatc aatgccgcca agcttctcct
     6961 cacaggcctg ggcaaacgcc gtcagctggc tgtaatcacg cacgtcgcag cgctgggtga
     7021 aaccatcacc gccagcctcg cgaaccatct gcagggtttc cgccaggccc ggctcgttga
     7081 catccgagag cgccaactgc cagccctcac gcgcccaacg cagggcaatc tcacgcccca
     7141 aacccgaacc cgcgccggtg atcatcatgc gattttgcat aggaaagctg ccttgtgctg
     7201 ctgtgattga tggggccagt gtagccgggg aaattatctt gcctgcacac catcatccat
     7261 ctgaataagg cggcctaagc tcaactcacg gccgccgcca gcgctgccaa agcaacacgc
     7321 cgtaaatcag gccattgagc agcagaacga acagccccaa gccaatctga atctgcggcg
     7381 tcaacccagc cgggtagatg atcggcagca gatagtgctc aacgaaacca ccgctatacc
     7441 ccgcctgccc cgcagccaga cgcaggctgt gctccagcgg tgtcagcgga cagggccaat
     7501 gcatcaactc caccaacaca ccccacaagg ccgccggcac atgcaaccag accaacccgc
     7561 gccaacgcca caccaacaag ccgcctaaaa ctgcaaatag aataaacagc agatgtacaa
     7621 ccaccactgc atctgccgcc actgccaacc aaaacataaa atgcctcgct gttaatcctc
     7681 aactcagcac tacatgaagc gcagcagttg taaaaaatac aatacgcaga aagcaaaaag
     7741 gccggttaat aaccggcctt aattaaacaa ctaaacgcgt cagttagtac tcaacttgtc
     7801 gcgattcctc tccaacgttg cctcaccaat acctttgact tccagcaact catccactga
     7861 agcaaagtcg ccatgctcat cacgataagc aacaatcgcc tgcgccttga cctcaccaac
     7921 ccccaacaac tcacgttgca gggtggcagc atcagccgta ttgagattga ctttgccaac
     7981 cggagccact tccgcagttt caacaacagg tactgactga gtctgcgttt cagcggcgac
     8041 aaccaccaag gaaattgaag taagaacagc gaacaacaga gaagacagtt tggcttttag
     8101 catacgaaga tcctttcgtt agttttgtca gaacgtggta cttccctctc cacgctctga
     8161 aaactagctg cagccataca cttgtcaaac aactccatgc attacttaag gatggtttga
     8221 agtaagaatg tatcttcaac acatatcagc acgaaaccat gccaaagcga tcaatccccc
     8281 catcgacaac accgtcgagc gtgccgcctg cgcctcgtca tcagccgcaa aactggctgt
     8341 aaacaacaga ccgaaaggcg ccaaggcagc gcacagatat gcagcttgtt ccaaaatgcc
     8401 aaaagcctag cgccacaccc gcgaataccg tcactggcca atagtagagg tgggtgtttg
     8461 gagcgctcac ataccctatg gctctagccg ccgcttcatg tgtatccagt caacaatctc
     8521 gccttccggc ttataaccac taaccgtctc acgcaacaac tgccgtacac ggtcataatc
     8581 atcacgttca acagcgtcca acaactcgct cagccggact ttgaaaagtt cccacggcag
     8641 atgctcctcg ttggcccgca tgatcatcgg gtgctcagtc ggactgacgt tatcgccgat
     8701 caataactcc tcatagagct tctcgccagg tcgcaggccg gtaaactcga tggagatatc
     8761 gccgtgaggg ttcttctcag agcgaacact caagccgctg agatgaatca ttttctccgc
     8821 cagctcagca atcctcaccg gctgccccat atccaataca aacacatcac caccctgccc
     8881 catggcaccc gcctgaatca ccaactgcga ggcttcagga atggtcataa agtaacgggt
     8941 gatattcgga tgagtgaccg taacagggcc gcctttgcga atctgctcat gaaacagcgg
     9001 tatgactgag ccggaagagc ccagtacatt gccaaagcgc accatggtaa agcgcgtctt
     9061 gttcagctgg cgcagtgctt tgtcatcctt aaacaagact ggcgcagact cacgactaag
     9121 agcctggagc accatttcgg ccaaccgttt ggtactgccc attacattgg tcgggcgtac
     9181 ggccttatcg gtggagatca gcacaaaatt actcacgccg gccctgatgg ccgcttgtgc
     9241 agtattcagc gtacctacca ggttattcag tacgccttcg gcgatattgt gctccaccat
     9301 aggcacatgc ttataggctg cagcgtgata gaccgtatcc accttccagg cttgcatcac
     9361 gtccaacaac ctatcggcat tacggattga accgaggata ggcactaacc gcagcggcag
     9421 cgattcacgc ttgatacgcg cctgcagctc actgtgaatg ctataaaggt taaactcgct
     9481 gtgctcgaac aacaaaagcg ctgcaggctg attgagcaga atttgccggc acaactctgc
     9541 accaatcgaa ccaccagcgc cggttaccat caccacttgg ttacggatac agcgctcaaa
     9601 cagcacctgc tgcggcggta ctgagtcacg ccccaacaag tcggcgatat caacttcctg
     9661 gacatcttca accgtcaccc ggccactggc cagatccata atgccgggaa tactgcgcac
     9721 atgcagaggg aaatgctcca gctcctccag aatctgccgc cgccgcccac gcgaggctga
     9781 aggcatagcc aagagaatct catcggcacc cgtttcattg atcatctgct ggatgtgctt
     9841 ggaggtgtac acccgcagcc cggcaatgct gcgattggcg atacc
//
LOCUS       DASRUM010000109        36202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_222341, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000109 DASRUM010000000
VERSION     DASRUM010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 36202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36202
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222341"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VFF22_07250"
     CDS             complement(<1..624)
                     /locus_tag="VFF22_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HZX16644.1"
                     /translation="MCELLGMSANVPTDIVFSFTGLMQRGGRTGPHRDGWGIGFYEGR
                     GLRLFQDPRASSESEVAQLVQRYPIKSEVVIGHIRQANVGRVCLANTHPFVREMWGRN
                     WCFAHNGQLADFAPQLDNYRPIGDTDSEAAFCDLLNRLRQAFPEPVAVERLLPTLVSA
                     CAGYRQLGVFNCLLSDGDWLFSFCSSKLAHITRRAPFGPAHLKDADMQ"
     gene            complement(617..1060)
                     /locus_tag="VFF22_07255"
     CDS             complement(617..1060)
                     /locus_tag="VFF22_07255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HZX16645.1"
                     /translation="MECVFCAIAAEQLPAERIYEDEHFIVLLDIFPLRPAHVLIVSRE
                     HAPFLKDLSTAACEQLLPLAQRMAAALRAAGYGEQGINFLINDGPTSNQHVPHLHLHL
                     IPRRSGDVMPLLWRALTRFLPLARKRIEARLVIEAQQLREALRNV"
     gene            complement(1146..1619)
                     /locus_tag="VFF22_07260"
     CDS             complement(1146..1619)
                     /locus_tag="VFF22_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX16646.1"
                     /translation="MTENDYLLAWGAYLLAAFGCLLVWFRLTSWMWRYVREPLRVLVV
                     VLLFSPTIVDPGKDQIAPAVAITAMDLLLDVGNNVWRAVADLSMYGLIMFTLYLLFVA
                     VRWPVERWWQARKGPLPIADDEPTLREMMARDEQDFGDTRYDARGDRRLRVEPRL"
     gene            1772..3802
                     /locus_tag="VFF22_07265"
     CDS             1772..3802
                     /locus_tag="VFF22_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZX16647.1"
                     /translation="MHHAPIARVDNAADPYRWLENRDSAEVLDYLKAENAYLEQQLSE
                     QLQLRETLFQEIKGRIRETDLSLPSPWGPYLYYQRTTAGDEYPRHYRCRKPADGSLTV
                     DEASEQLLLDPNALADGGFISLGAFSISPDHQRLAYSLDTSGEETYQLFVKELADDSL
                     IELPFEECDGSMTWANDSQTLFFGELDDTHRPHKLYRHRLGSDAAELVFHEPDGRFFL
                     SCYRSSSERQLILQLGSKTTSEAWVLDAATPQAAFSCVAPREEGHEYDVDHGTLNGEW
                     SWLIRSNQHGINFALFSASEAAPSRSHWQLLRAHDPEVMLEGFSLNQQALVLSLREGG
                     LPIIEVQPQGQAAYRVQLPDAAYSLYVQDSLEFDSPAIRLRYEALNRPAQVRQLELAT
                     GAQLVLKQTPVLGPFDADDYVSQRLWATAADGSQVPISLVGKREVLGTSAPLYLYGYG
                     AYGESLDPWFSHARLSLLERGFIFAIAHVRGGGELGEAWYRAGKLEHKQNSFSDFIAC
                     AEHLIASGYTSAEQLVISGGSAGGLLMGAVLNQRPELFAAAIAEVPFVDVLNTMQNPD
                     LPLTVTEYDEWGDPNQPEVFERIKAYAPYENVQVQAYPAILAVAGYNDSRVQYWEAAK
                     WVAKLRAQKTDNNLLLLKTELDAGHGGMSGRYQALKDAALEYAFILKVLGLV"
     gene            4022..5644
                     /locus_tag="VFF22_07270"
     CDS             4022..5644
                     /locus_tag="VFF22_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008026383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX16648.1"
                     /translation="MKNWKIRTHLFLLSGCLLFGLLCVGGLGLYGLQATVKGLQTVYL
                     DRVVPLRDLKLIADLYAVNIVDATHKARSGELDENTAQQLIEQAQVRIEETWQAYLAT
                     ELIREESQLIEQINPLMQQARQPLNELQSLLNRGDKVGVEAFASRRLYPLIDPLSEKF
                     AELIDVQLLEAKHQYQLGEAAYASNLNLSISVLLIALLVGLAQVFYFIRLMSRQLGAE
                     PGELEEISAHIAEGRLGGKTLGNQVMTGVMKSVQSMRRGLQDMIGNIGEASEQIECAT
                     LQLAASSEQVLTSANVQSDTASAMAATVEQLAVSINHIAENAQQTYDMAKKAGDMTDA
                     GIQVMARATTEMHKIAELVAESSQDVETLAAQSRNISAIVDVIRGIAEQTNLLALNAA
                     IEAARAGEQGRGFAVVADEVRSLAGRTAQSTTEIIALVDAIHSGMHKAKSSMAAGCER
                     VSIGTQLVDQAGSSMTGIRLALNESLQAVSVISLSLQEQRAASEQVAMSVERVAQIVE
                     ENSAAQGGIVQAIQSLQAMSGRLQGVMRRFSF"
     gene            complement(5746..6045)
                     /locus_tag="VFF22_07275"
     CDS             complement(5746..6045)
                     /locus_tag="VFF22_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16649.1"
                     /translation="MRLTTLLLVLTTLASTSLLAQQPPKMPATSPAPGAPGTATPQPY
                     PSTAPQHLPAPSRNNPPLLKQPSPPQSSQPRSDQELPLLREQRERNAQPLPKPDE"
     gene            6170..6541
                     /locus_tag="VFF22_07280"
     CDS             6170..6541
                     /locus_tag="VFF22_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007942195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HZX16650.1"
                     /translation="MSSDKSPSATGKLDRILAEAQRSREEGYRDKALKMYPHVCGRCA
                     REFSGKRLSELTVHHRDHNHDNNPQDGSNWELLCLYCHDNEHSRYTDQQYFSEGSLST
                     PKTAQATHNPFADLAARMKKQ"
     gene            6718..7188
                     /locus_tag="VFF22_07285"
     CDS             6718..7188
                     /locus_tag="VFF22_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016498381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZX16651.1"
                     /translation="MANKRYACIGLFNPKSPENVGSVMRAAGCYSAASVFYSGKRYAR
                     ARDFITDTQRIYMDIPLIGVEDLQQIIPIGCTPVAVELVEGARPLPQYTHPDRAIYIF
                     GPEDGSLSKEVLDWCEDVIYIPTQGCMNLAATVNVVLYDRMAKGINTKSGPGLK"
     gene            complement(7388..8614)
                     /locus_tag="VFF22_07290"
     CDS             complement(7388..8614)
                     /locus_tag="VFF22_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16652.1"
                     /translation="MLIRRLLLATALLSLSTPLWAKKVDLDYHVRFLPDTEEAEVSLI
                     LEDGAPVISLAFNLGEQGAYSDFKADGQWTQNSAELGTWQPGKGKSTLTYRVRINNER
                     SAGRFDALINKDWALLRGDDLVPAVKLRQEDKTELVSRLQFDLPKGWKSVETGWPRIG
                     KNKFRIDNPQRKFDRPTGWMVAGKVGTRRAKLGETDVAIAAPVGEGMRRMDVMTLLTF
                     IWPHAQDVFPRDPAKLLIVGAGDPMWRGGLSAANSYYMHTDRPLVSENGTSSLVHELV
                     HVFSRIKARDRSDWITEGLAEYYAIELMRRAGGLSEERYQKIREQLIRWSKPVDSLRT
                     NNSSGPVTARAVLLLQELDREIRQATKGTTQPRSLDDVTRGLMRLDKASTKDFIEISE
                     NVMGSASKTLDNKLLR"
     gene            complement(8700..9149)
                     /locus_tag="VFF22_07295"
     CDS             complement(8700..9149)
                     /locus_tag="VFF22_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HZX16653.1"
                     /translation="MAAKVEPFWKRKTLEQLDQDEWESLCDGCGLCCLQKLEDEDDGS
                     VYYTRIACKLLDLQTCRCTDYSNRRKFVADCIQLTPAQADQFQWLPPTCAYRLVSEGK
                     DLLLWHHLVCGDPEQVHKQRISQAGRMLSENSVAEDDWEDHMIFRAG"
     gene            complement(9234..10190)
                     /locus_tag="VFF22_07300"
     CDS             complement(9234..10190)
                     /locus_tag="VFF22_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018926763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HZX16654.1"
                     /translation="MPATANAPVRVLIAEAEHALYAELLREQAPELELFGSDRPAELA
                     EYAASYPLWLGQPDLLAPLLRQGSRPQWLQSTWAGITPLLAGDLPTNYALTRAVGIFG
                     QVMAEYVLCHLLAHERQLLSRLAAQVEQRWDNRLPCSLAGRKALIVGTGDIGLSVAQF
                     LAPFGIDLLGIASSPRNLPPFRQVAGLDELPSLVGEADYLINLLPDTPATRDIYDAAL
                     FAACKPSALFINAGRGVAVVDQALVSALQQGQLAGAVIDVCREEPLPAGHPFWDAPRL
                     LLTGHSSAPTDPHLLVKLFVDNLVRWQAGSALRGHVDFVRGY"
     gene            complement(10196..10489)
                     /locus_tag="VFF22_07305"
     CDS             complement(10196..10489)
                     /locus_tag="VFF22_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020289385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HZX16655.1"
                     /translation="MKRICSIYKSPRKSEMYLYVLRSDGLKRVPENLLLAFGPPALAF
                     EMVLTPERVLAREDIHKVLANLDEQGYHLQMPPPEDEYIEHLPDELLRRNDPM"
     gene            complement(10486..11619)
                     /gene="rnd"
                     /locus_tag="VFF22_07310"
     CDS             complement(10486..11619)
                     /gene="rnd"
                     /locus_tag="VFF22_07310"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HZX16656.1"
                     /translation="MAIDIQWIGDNASLAQHCATWRSLPFVAVDTEFMRVDTFYPIAG
                     LLQVSEGERAYLIDPLLISDWAPFAELLQDSAVVKVLHACSEDLEVFLRLTGSLPTPL
                     FDTQLAAGYLNLGFSMGYSRLVQAVLNIELPKGETRSDWLQRPLSATQVSYAAEDVLH
                     LAEVYAQLKAQLSAEKYAWVLEDGAELVSNLKREVDPELAYREAKLAWKLNRQQLAVL
                     KALCTWREGQARKRNQPRNRIIREHSLWPLARSQPDNLVALARIEDMHPKTVRQDGEF
                     LLQLIKQAAATPAEDWPEALPEPLPLDAASLLKKLRAIGQREGERLNIAPELMLRKKT
                     LEALLKTGYPNGPYQLPESLRGWRRELMGQALLDCLAATGESA"
     gene            11777..12838
                     /locus_tag="VFF22_07315"
     CDS             11777..12838
                     /locus_tag="VFF22_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZX16657.1"
                     /translation="MKKLLGLLVALIAAAAIYLAVTPSPIDPLAWEAPAIPPMTGVLE
                     HNDTLMKAELLGRGQVHGPEDTAVDTQGLVYAGLHDGKIVRIKADGSVETFVDTKGRP
                     LGMDFDAAGNLIVADAYKGLLSIDAQGQIKVLTTEAEGVAFKFTDDLDIARDGTIYFS
                     DASQRFEQPDYLLDLLEARPHGRLLSYNPATGETKVLLKDLYFANGVALSAAEDFVLV
                     NETYRYRITRYWLSGDKAGTHDIFIDNLPGLPDNLQGDRNGTFWVALPTPRKADADML
                     HRSPWAKSQLAKLPRAFWPKAVPYGFAIALNEQGEITQSLHDTSGTHLRMITSVKPVG
                     DYLYFGSLDNDRIGKLKIR"
     gene            complement(12905..13588)
                     /locus_tag="VFF22_07320"
     CDS             complement(12905..13588)
                     /locus_tag="VFF22_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017336998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HZX16658.1"
                     /translation="MKRFVLLDTAPIPDGSGALCLFEYGEDFVIKIAGGDGGQLMNTR
                     MHGSEDALAAIPCKKVAGRPDSRVLIGGLGMGFTLAAALQNLGKTAEVEVAELVPGVV
                     EWNRGPLGAKAGYPILDPRTRVIQDDVANCLKSADQRYDAIMLDVDNGPEGLTQKRNS
                     WLYSSQGLQRCHQALRNKGVLAVWSASADRVFSDKVRKAGFKVEEVQVYAHGNKGTRH
                     TIWIAEKIK"
     gene            13896..14750
                     /locus_tag="VFF22_07325"
     CDS             13896..14750
                     /locus_tag="VFF22_07325"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HZX16659.1"
                     /translation="MPLAQLISAAQLQRRLDEPDLLILDCRFALDDLTYGQRSYTEGH
                     IPGAQFADLERDLSGPIHKGITGRHPLPEPAQLLQRLREWGLNNHSEVVLYDDGPGAF
                     ASRAWWLLAWLGKRECVYLLDGGLKAWREAGGALTQDLPAQAAGTFSGNADASLLVSA
                     AQLQQRLGDSQLTLLDARGLPRFRGEVEPIDPVAGHIPGAQCAAFTDNLDSDGHFLSP
                     EQLRQRFAAQLGERPASELVAYCGSGVTACHNLFALCLAGYPLAPLYAGSWSEWITDP
                     ARPVATGG"
     gene            14987..15466
                     /locus_tag="VFF22_07330"
     CDS             14987..15466
                     /locus_tag="VFF22_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16660.1"
                     /translation="MGIAACELSQYVIRPTLLYLGRHSNAAEALLLGAAASQSGLGSA
                     LDDKHGHGLYRIAAQRHQQLWDEHLALDPDLASQVRGLASQHAFLAAPHLELTVNLRY
                     ATAIAWMLIEAEHRELPAADDLLGMAQVWRRVFRPHGRTKDFIAAWQRCIGSLSQAA"
     gene            15630..16880
                     /locus_tag="VFF22_07335"
     CDS             15630..16880
                     /locus_tag="VFF22_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003369671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HZX16661.1"
                     /translation="MTRRVVKTCLSLALASAASHGFASGFAINEQSVSSMGTAFAGRS
                     SSADDASTVFGNPAGMARIKREQVSGGIALIHAKTEIDNASGSVSGSNDGDMVPFIGA
                     PMGYYVKPLDDNWSFGLGLYVPFGLVTDYERNFQGRYHGDRSEVRVITLQPTLSYRFN
                     EHLSIGFGPTINRIDGELTSAINNPIPGAGDGKVKIKGDDTALGYNVGVLFEITPQTR
                     FGMTYHSKVDYRLKGDTTVSGPGFVIGSSAGTYDASLELTTPESVDFSVTHELDADWT
                     LYAGSTWTRWSRLQEIRADNKGVGGLLAGNLSSISEPQNWHDTWAHAVGAAYKLNPQW
                     TLRAGLAVDQSPTNNADRSPRIPTGDRTILSLGTAWSPNDDVTIDLAYSYLMEDDAHI
                     ERDDYRATYKNTAHGLGTQVTYRF"
     gene            16950..17567
                     /locus_tag="VFF22_07340"
     CDS             16950..17567
                     /locus_tag="VFF22_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZX16662.1"
                     /translation="MTALCTALPEQLSLLLVDDHRIFLDGLSLALAPLCADLQIRTAE
                     SAAAAEACLQHATFDLILLDLRLPDMPGLELLQRWQQQGRMTPVAILSASDSNLDAQA
                     ALAAGALGFIPKSANSDELRQAVTRVLLGETLPAPQTGDKPQLTPRQMEILQLLAEGL
                     PNKSISRQLGVSEDTVKTHLKSLFQEFSVHTRTACVSAARQRGWL"
     gene            complement(17536..19314)
                     /locus_tag="VFF22_07345"
     CDS             complement(17536..19314)
                     /locus_tag="VFF22_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX16663.1"
                     /translation="MSLERQEALLLAQQRRGYRRLRFTDVLEDEFVEHRVQRIRRRLL
                     LIISTAVVFQLVYTVLDLLLLPLNASLTTLPVRMLGIFGALLSFFYCRRPQSPPRRAL
                     LVYVAAYALNGVCVAVIIHLCWSLGVAMPYDGLFLVLLFGYVLLGVSFRSVSLASWGV
                     CGLFLWLGLIATGGSVALGYQGLFLFCANLIGSVGAYLQEHGQRGAWLNLRLLDLARQ
                     RAEADDARKLRLLAAASHDLRQPLNAMGLYAQHLLEQNQDPGVHRISSRLAIAVEQLS
                     RLLQSLFDYTRLTLPGGVQAKPEVFALRPLLARLSGESRAEADAQGVELQLQCADLWV
                     RSDPLLLERVLRNLLSNALRHAQARHVWLHARVEQGVVLLEVGDDGQGLSEEEQSQVF
                     EEFRQLDNPGRNSERGLGLGLAIVQQLAQLLGHPLQLESSPGAGARFILQLPQAEAGE
                     WQAPAAVAAALSGRVLLLEDDAAGREALSGLLQRWGCEVWACADPAEALQCLPEAQPQ
                     VLISDYRLAATEDGLRVIERLREEAGRMLPALLITADVSADLQERCTRAHVTLLGKPL
                     LPARLRQALAVLLPAELKASPVAAPH"
     gene            complement(19318..20388)
                     /locus_tag="VFF22_07350"
     CDS             complement(19318..20388)
                     /locus_tag="VFF22_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HZX16664.1"
                     /translation="MALSLRSTIPLLLLAGSVAGLTLYWQWPATAVAVAPAAVVTATA
                     PLTVAPSPLLPVAQPDTAAAGSPAHLPGLQGTDVDGELKADSAGNLQLNLALRDYLDY
                     FLSAADQAGLETVVEAMLADARTRLPEPALGQFIGLLGDYMDYKRASLALLQQPLSAA
                     QQSTPDGQLVALQRAFEQLAQLRRTHFSSAATEALFGAEEAYGRYTLDNLALMARDDL
                     SEHGKALAQERLRAQLPEAMRASEERQAQAQALQVQTDKLWQEGASEEQVRQLLALTY
                     DPPTVERLLSEQRNERAWQQRYATYQQELAALQGNGLSAADQQQEQQRLRQRLFSAED
                     QHRVETYDAIAAKQQDSSSPEL"
     gene            complement(20437..21363)
                     /locus_tag="VFF22_07355"
     CDS             complement(20437..21363)
                     /locus_tag="VFF22_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZX16665.1"
                     /translation="MRRVITTALAAVALVGAVEAQANYTKTKYPIVLVHGVTGFNTIG
                     GLINYFHTIPWNLERDGAKVYVASVSALNDSEQRGAALAQQIVPWAGANGGKVNLIGH
                     SQGSPTSRVAASLRPDLVASVTSVNGVNKGSKVADVLRGVIPPNSAIEGGANALANAL
                     GAVINLLSGSSNPQSGIDALKTLTTAGTTALNGRHPWGVNSSSYCAKSTEVHNVRGHN
                     IRYFSWTGNTSYTNVFDAVDPFLAITGLAFGSEKNDGLVGVCSTYLGQVIGDGYNMNH
                     VDAINHLFGIRGWTEPVSLYRQHANRLKNKGV"
     gene            complement(21471..22022)
                     /locus_tag="VFF22_07360"
     CDS             complement(21471..22022)
                     /locus_tag="VFF22_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003404262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HZX16666.1"
                     /translation="MWRFSLLVVVLLSAGVGPAQAAECPALLQGEVQKLRSEERIDLC
                     AQFAGKPLIVVNTASFCGFTPQFKGLEALYQRYKELGLQILGVPSDDFRQEADSSEET
                     AKVCYVNYGVTFPMSETQRVTGSDALPLFKELAAQSQAPRWNFSKYVIDAQGRVIASF
                     PSSTTPDDPALLAAVEKAIASQP"
     gene            complement(22042..22167)
                     /locus_tag="VFF22_07365"
     CDS             complement(22042..22167)
                     /locus_tag="VFF22_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017130944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16667.1"
                     /translation="MNTDYGWNTALVMGGMLALGLAWWGWLQSGLAFLQLGLGVC"
     gene            complement(22164..23363)
                     /locus_tag="VFF22_07370"
     CDS             complement(22164..23363)
                     /locus_tag="VFF22_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX16668.1"
                     /translation="MGRGSMWILLGASLILALSLGIRHGFGLFLPPMSAEFGWGREVF
                     AFAIALQNLIWGLTQPFTGALADRFGAKRTVIVGGILYAIGLGCMGLADSPLSLSLSA
                     GLLIGIGLSGTSFSVILGAVGRAVPMEKRSMAMGIAAAAGSFGQFVMLPGTLGLLSWL
                     GWSVALMALGLLVALIIPLAAMLKDTPLPLTGQEQTLGEALREACSHSGFWLLALGFF
                     VCGFQVVFIGVHLPAYLVDQHLPALVGTTVLALVGLFNIFGTYIAGWLGGRMSKPRLL
                     SALYLARAVVIVAFLMTPLTVWSAYAFGVAMGLLWLSTVPLTNGTVATMFGVRNLSML
                     GGIVFLFHQLGAFLGGWMGGYLYDHTGSYDLVWQIAVLLSLLAAALNWPVRELPVARL
                     QQQEANA"
     gene            23580..23912
                     /locus_tag="VFF22_07375"
     CDS             23580..23912
                     /locus_tag="VFF22_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HZX16669.1"
                     /translation="MSSPQYASCMQACSACAIACETCASACLEENDVSMMATCIRLDR
                     DCADMCHLAQVLMARKSALVDEFCRLCAVACQACAEECLKHQHEHCQQCAQACQRCAD
                     ECNALAQS"
     gene            complement(23936..24673)
                     /locus_tag="VFF22_07380"
     CDS             complement(23936..24673)
                     /locus_tag="VFF22_07380"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011333147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HZX16670.1"
                     /translation="MTPLLIALQFLTRLPIRLPGMPTPQQIGRSLLWYPLVGLLIGLL
                     LVAASYMLGDRPVMLQAAVLLTLWVALSGGLHLDGLADSADAWAGGFGDRERTLTIMQ
                     DPRSGPIAVVVLVLLLLLKFVALATLLAANSALSALLLVPLLGRSAMLALFLTTAYVR
                     PGGLGQAMAEHLPRALGWRVLIASALLCLLCGGLSGLLALLIACALFYCVRRLMCQRI
                     GGTTGDTAGALLETLECVVLLGLAISA"
     gene            complement(24670..25245)
                     /gene="cobC"
                     /locus_tag="VFF22_07385"
     CDS             complement(24670..25245)
                     /gene="cobC"
                     /locus_tag="VFF22_07385"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008001258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase family protein"
                     /protein_id="HZX16671.1"
                     /translation="MTLYLELLRHGETELGGGLRGSLDDALTETGWAQLRAAVAEAGP
                     WDRLISSPLQRCARFAEELATEHGVPLSLEPDLQELHFGAWEGCNTAELMQTSAEDLG
                     RFWSDPYGFTPPEGEPLLAFEARIFSALQRLQACHVGERLLLVTHGGVIRLLLARARG
                     LPRGDLLQINVAHAARFRLSLDKAGELRELA"
     gene            complement(25242..26297)
                     /gene="cobT"
                     /locus_tag="VFF22_07390"
     CDS             complement(25242..26297)
                     /gene="cobT"
                     /locus_tag="VFF22_07390"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HZX16672.1"
                     /translation="MSEQWWQQGCQALDMPVRSKAQARQLELTKPAGSLGQLEQLAID
                     LAAMQGTQRPQVEQLWISIFAGDHGVVAEGVSAFPQAVTGQMLRNFVGGGAAISVLAK
                     QLSATLEVIDLGTALPLEPLRGVRHLHIGAGTANFAQGPAMTAAQLLLALEAGQASVL
                     RAREAGSQLFIGGEMGIGNTTAATAMACLLLECPAQDLVGPGTGLDSAGVAHKAEVIE
                     IALELHRPAISEPIDILQRLGGFEIAALSGAYLACAQQGITALVDGYICSVAALLAVQ
                     LNPACRDWLLFAHTGAEPGHQRVLKALQAEPLLDLGLRLGEGSGAALAVPLLQLACSL
                     HNEMATFAEAAVAERPA"
     gene            complement(26294..26815)
                     /gene="cobU"
                     /locus_tag="VFF22_07395"
     CDS             complement(26294..26815)
                     /gene="cobU"
                     /locus_tag="VFF22_07395"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HZX16673.1"
                     /translation="MHELILGGARSGKSRLAERLAAESGLAVTYIATSQPLDGEMNTR
                     IQHHRERRPAHWGLVEEPLALARVLREQAAADKCLLVDCLTLWLTNLLMLDNPARLSA
                     EREALLDCVNGLPGRILLVSNETGLGVVPLGELTRRYVDEAGWLHQALAERCERVVFT
                     VAGLPMVLKGNAL"
     gene            complement(26808..28265)
                     /locus_tag="VFF22_07400"
     CDS             complement(26808..28265)
                     /locus_tag="VFF22_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HZX16674.1"
                     /translation="MSTLMVQGTTSDAGKSTLVTALCRWLKRQGVSVVPFKPQNMALN
                     SAVTADGGEIGRAQAVQAQAAGLVPHTDMNPVLLKPNNDTGAQVIIHGRALSCMDAVA
                     YHDYKKVAMQAVLQSHQRLSAAYPVVMVEGAGSPAEINLRAGDIANMGFAEAVDCPVI
                     LIADIDKGGVFAHLVGTLALLSESEQARVQGFVINRFRGDIALLQPGLDWLEQRTGKP
                     VLGVLPYLMDFHLEAEDAIDIRQSAKVAERIKVVVPVLPRISNHTDFDPLRLHPQVEL
                     TFVGPGQAIPASDLIILPGSKSVRSDLAFLRQQGWPAAIAKHLRYGGKLLGICGGLQI
                     LGTQIDDPHGLEGAAGSSPGLGLLEFSTVLEPEKQLRNVRGQLCLENAPVSGYEIHAG
                     VSSGPGLNAVVRLDDGRADGGLSADGQVLGTYLHGLFETPEACSALLRWAGLREVQQV
                     DYHALRERDIERLADLVEHHLDTARLRELCGVGHA"
     gene            complement(28262..29254)
                     /gene="cobD"
                     /locus_tag="VFF22_07405"
     CDS             complement(28262..29254)
                     /gene="cobD"
                     /locus_tag="VFF22_07405"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HZX16675.1"
                     /translation="MLEHGGRLRAAALRYGIALADWLDLSTGIAPYGWPLPVIPASAW
                     ARLPEQHDGLEIAARDYYGVSNLLPVAGSQAAIQALPRLVRDARVGIVSPAYAEHAAA
                     WQREGHRLLELSEGGIERALERLDVLLLVNPNNPTGRRIEPQRLLDWHARLAERGGWL
                     VVDEAFIDCAPDFSLAAFSDRPGLIVLRSFGKFFGLAGIRLGFVLAQPAVLERLAELL
                     GPWTVSGPSRVVATALLQDHAGQRLQRERLLADGLRLAVLLSAQGFAPTGGCALFQFC
                     CTPRAVELHEFLAAKGILTRLFASPLSLRFGLPADEAGWQRLQQALELFAKEAR"
     gene            complement(29263..30171)
                     /gene="cbiB"
                     /locus_tag="VFF22_07410"
     CDS             complement(29263..30171)
                     /gene="cbiB"
                     /locus_tag="VFF22_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HZX16676.1"
                     /translation="MSLVLSTCAGVALDAIFGEPKGWHPLVAFGRMAERIEQRFNPAG
                     GGLRSHGVSAWCLAVLPLTLLAWLLSQISGLGWAVEILALYAALGLKSLGEHAMPVAQ
                     ALRLGDLRLARERVGYMVSRNTAQLDAAGVARAGSESVLENGSDAVFAALFWFVIAGA
                     PGVVLYRLSNTLDAMWGYRNERFERFGWAAAKIDDVLNYLPARLVALTYALLGKTMLA
                     LRCWRTQAPLWDSPNAGPVMASGAGALGVSLGGAAEYHGELHQRPELGAGPQPRARDI
                     ERALNLVCGGVLLWLALLLLWGAFYA"
     gene            complement(30168..30815)
                     /gene="bluB"
                     /locus_tag="VFF22_07415"
     CDS             complement(30168..30815)
                     /gene="bluB"
                     /locus_tag="VFF22_07415"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HZX16677.1"
                     /translation="MSGLAFSPDERAAVYRAIAERRDMRHFSGGEVAPELLARLLEAA
                     HQAPSVGLMQPWRFIRITRPALREAIHGLVEEERLRTAEVLGERSDEFMRLKVEGIRD
                     CAELLVVALMEGREAHVFGRRTLPEMDLASLACAIQNLWLAARAEGLGMGWVSLFEPQ
                     ALADVLGMPAGSKPLAVLCLGPVTEFYDAPMLVQQGWSEPRPLSELLFENQWGTP"
     gene            complement(30812..32116)
                     /locus_tag="VFF22_07420"
     CDS             complement(30812..32116)
                     /locus_tag="VFF22_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019407264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HZX16678.1"
                     /translation="MSARSCPALLIAAPASGQGKTTVTAALARLHTRQGKRVRVFKCG
                     PDFLDPMILARASGAPVYQLDLWMVGAAESRKLLWEAAGEADLILIEGVMGLFDGTPS
                     AADLARHFGVPVLAVIDGSAMAQTFGAMAHGLATFQPDLPFSGVLGNRVGSTRHGEIL
                     RDALPASIRWYGALPRSAEVGLPSRHLGLVQAGELADLDARLDAAADALAASADTSLP
                     SPVSFATPEQARPAQLLKGVRIGVARDSAFAFLYQANLDLLHELGAELQFFSPLTDEQ
                     LPDVDSLYLPGGYPELHLVTLEANQPMAQAIRAHHAAGKPIHAECGGMLYLLDKLTDK
                     QGASGQMLGLLNGEATLQPRMTAMALQQAELPEGLLRGHSFHYSALSSDLQPLTRGEC
                     PNYKRTAEAVYRQGRLTASYIHFYLPSDPVAAAALFCPGALA"
     gene            complement(32116..32727)
                     /gene="cobO"
                     /locus_tag="VFF22_07425"
     CDS             complement(32116..32727)
                     /gene="cobO"
                     /locus_tag="VFF22_07425"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZX16679.1"
                     /translation="MTESIERDARHKARMARKKALIDEKIAQAQDEYGLLLVHSGNGK
                     GKSSSAFGMVARALGHGLKVGVVQFIKGAASTGEETFFRRFPDEVSYHVMGEGFTWET
                     QDRQRDIDKAREAWAVAARLLNDPAIGLVVLDELNIALKYGYLELDAVLADIEARPLL
                     QHVVVTGRGALPGMIEAADTVTEMNLVKHAFKAGVKAQKGVEF"
     regulatory      complement(32837..33061)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(33125..33445)
                     /locus_tag="VFF22_07430"
     CDS             complement(33125..33445)
                     /locus_tag="VFF22_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16680.1"
                     /translation="MPEPIRLPEQPAACELCARTTTLTRHHLIPKALHNKVYVQKRFG
                     KSERISATLWVCRACHNQIHRLFSEKELALTYNTRDSLLSDERLRTFVEWLASKPAGF
                     TPRH"
     gene            complement(33452..34114)
                     /locus_tag="VFF22_07435"
     CDS             complement(33452..34114)
                     /locus_tag="VFF22_07435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016702505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX16681.1"
                     /translation="MTNRTLNLDSALYQYLLDVSLRETPLLKRLREETAQLSMARWQI
                     APEQGQFMALLVQLTEARRIVEVGTFTGYSAICMAQAMPEDGRLICCDIPGEYNATAE
                     RYWREAGLSARIEQRLAPALQTLAALEAEGLSGSIDLLFIDADKANYPAYLEHALRLV
                     HKGGLILFDNTLWSGRVLEANPESADTRAIQALNLALRDDPRIDLSLLPLGDGLSLCR
                     KR"
     gene            complement(34142..34666)
                     /locus_tag="VFF22_07440"
     CDS             complement(34142..34666)
                     /locus_tag="VFF22_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019579635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HZX16682.1"
                     /translation="MTAIARLSLIVLAALLSACASNAPAPTPKPIVHAPIGSFNGNAE
                     DVLFRALGLVGTPYRYGGNTPDGGFDCSGLIGYVYRDAAGISLPRSTRELISMHAPAV
                     GRDRLQSGDLVFFATNGGSQVSHAGIYVGDGRFVHAPSSGGTVRLDSLSNTYWQRTYL
                     NAKRVFTPELASNP"
     gene            complement(34827..35441)
                     /locus_tag="VFF22_07445"
     CDS             complement(34827..35441)
                     /locus_tag="VFF22_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HZX16683.1"
                     /translation="MLKRLAPLVPIALSVVLAACANHAPQPQLATQPAVFSSTELAEF
                     DEQAKEAALGDFTEDKSYKLPQLADSILTHGLSLVGTRYRYGGSTVKSGFDCSGFIGY
                     LFKEELGMKLPRSSRDMINIDAPQVSRDELEPGDLLFFSTNGRNRVSHAGIYMGDNQF
                     IHSSSSRSGGVRVDSLDDRYWSRTFIEAKRALALAPSESLVRHP"
     gene            complement(35518..>36202)
                     /locus_tag="VFF22_07450"
     CDS             complement(35518..>36202)
                     /locus_tag="VFF22_07450"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HZX16684.1"
                     /translation="LSADSGLPTYRGLGGLYNGHTAEGLPIEEALSGSMLQRDPALCW
                     KYLAELGKACLAAQANAGHYAIAELQRRKPQCWLLTQNIDGYHRAAGSPMERVIEIHG
                     ELAPLYCQSCGAVDTELAEHLERPLPPKCRQCAGVLRPPVVLFEEMLPEQAIDTLYTE
                     VRKGFEVVISIGTSASFPYIVEPLLRTRQAGGFTVEINPQRTDLSDRVNAHLQGRALD
                     VLPELLSHI"
BASE COUNT         7492 a        11762 c        10730 g         6218 t
ORIGIN      
        1 ctgcatatcg gcatctttca aatgcgctgg gccaaacggc gcccgccggg tgatatgcgc
       61 cagcttgctg ctgcagaagc tgaacagcca gtcgccatca ctgagcaggc aattgaatac
      121 gccgagctga cgatagcccg cacaggcact gaccaaagtg ggcagtaacc gctctaccgc
      181 aaccggctcg ggaaaggcct gacgcaggcg attgagcaaa tcacagaagg ccgcttcgct
      241 gtcggtgtcg ccaatcggcc ggtagttatc cagttgcggg gcaaaatcgg ccaattggcc
      301 gttatgcgca aaacaccagt tacgccccca catctcgcgt acaaacggat gggtgttggc
      361 caggcagacg cggccaacat tggcctggcg gatatgcccg atcaccactt cgctcttgat
      421 cgggtagcgc tgcaccagct gcgccacttc cgactcgctg ctggcacgcg ggtcctgaaa
      481 caggcgcagg ccgcgccctt cataaaaacc gatgccccag ccatcgcggt gcgggccggt
      541 ccgcccgccg cgctgcatca ggccggtaaa gctaaagaca atatcggtcg gcacattggc
      601 gctcatgccc agcaattcac acattgcgta acgcctcgcg cagctgctgc gcctcaatca
      661 ccagacgggc ttcgatgcgt ttgcgcgcca atggtagaaa acgcgtcagg gcgcgccaca
      721 gcaacggcat aacatcgccg ctgcgccgtg gaatcagatg cagatgcagg tgcggcacat
      781 gctgattgga ggtcggcccg tcgttgatca agaaattgat gccctgctcg ccatacccgg
      841 ccgcgcgcaa ggcggcggcc atgcgttgcg ccaggggcag caactgttca caggcagcgg
      901 tggagaggtc tttgagaaag ggcgcgtgtt cgcggctgac aatcagcaca tgggccgggc
      961 gcagggggaa gatatccagc aggacgataa agtgctcgtc ctcataaata cgctcggcag
     1021 gcaattgctc agcggcaatt gcacagaaca cacactccat aacccctcct tatttctggt
     1081 gggccatgct gaacggctgt gtgctttggc gcaagggcag cgacgtcagg cgacgccgca
     1141 tcgccttaca aacgtggttc tacgcgcagg cggcggtcgc cgcgggcatc gtaacgggtg
     1201 tcgccgaaat cctgctcatc gcgcgccatc atttcgcgca gggtcggttc gtcatcagca
     1261 atcggtagag gccctttacg cgcctgccac caacgttcca cgggccagcg tacggcgaca
     1321 aacagcaggt acagggtgaa catgatcagg ccgtacatgc tcagatccgc caccgcgcgc
     1381 cagacgttgt tgcccacatc cagcaacagg tccatggcgg tgatcgccac cgcaggggcg
     1441 atctggtctt tgccgggatc gacgatggtc gggctgaaca gcagtaccac aaccagcaca
     1501 cgcagcggct cgcgcacata gcgccacatc cagctggtca ggcgaaacca caccaacagg
     1561 caaccaaaag ccgccagcag ataggcgccc caggcaagca ggtagtcgtt ttcagtcata
     1621 aaacgcaaat tctctggcat tgaagaccgg ccggcgcatg cgcataagcg cgtagatttc
     1681 agcagccgac gggcgaagtg gcgcttatga taacggcttt tgcctgctta ggcgcccctg
     1741 ctgtcggccg ccaccccaac ggagttaccc catgcaccac gcacccatcg cccgcgtcga
     1801 caacgctgcc gacccgtatc gctggctgga aaaccgcgac agcgctgagg tgcttgatta
     1861 cctcaaggcg gaaaatgcct acctggaaca gcagctcagc gagcaactgc aactgcgcga
     1921 aacactgttt caggagatca aggggcgcat ccgcgaaacc gacctaagcc tgccgtcgcc
     1981 ctggggccct tatctgtatt accagcgcac caccgccggc gatgaatacc cacggcatta
     2041 ccgctgccgc aaacccgccg atggctcgct gactgttgat gaagccagcg agcagctact
     2101 gctcgacccc aatgccctgg ccgatggtgg ctttatctcg ctcggcgcct tcagcatcag
     2161 ccctgaccac cagcgactgg cctacagcct ggataccagc ggcgaggaaa cctatcagct
     2221 gttcgtcaag gaactggccg acgacagcct gatcgagctg ccgttcgagg agtgcgatgg
     2281 cagcatgacc tgggccaatg acagccaaac gctgttcttc ggcgaactgg atgacaccca
     2341 ccggccgcac aagctgtatc gccaccgcct cggcagcgac gcggccgaac tggtattcca
     2401 cgagccggat gggcgcttct tccttagctg ttaccgcagc agctccgaac gccaactgat
     2461 cctgcaactg ggcagcaaga ccaccagcga agcctgggtg ctggatgctg ctaccccgca
     2521 agccgctttc agctgcgtgg ccccacgcga agaaggccac gaatatgacg tcgaccacgg
     2581 caccctgaat ggcgagtgga gctggctgat ccgcagcaac cagcacggca tcaacttcgc
     2641 cttgttcagc gccagcgaag ccgccccgag ccgcagccac tggcaactgc tgcgcgccca
     2701 tgacccagaa gtgatgctgg aaggctttag cctcaaccag caagcgctgg ttcttagcct
     2761 acgagaaggc ggcttgccga ttatcgaagt gcagccacag ggacaagcgg cctatcgcgt
     2821 gcagctaccg gacgccgcct acagcctgta tgtgcaggac agcctggaat tcgacagccc
     2881 ggcgattcgc ctgcgctatg aagcgctcaa ccgcccggcg caggtgcgtc agctggagct
     2941 ggccactggc gcgcaattgg tgctcaagca aaccccggta ctcggaccat tcgatgccga
     3001 cgactatgtc agccagcgcc tatgggccac ggccgccgat ggcagccagg tgccaatcag
     3061 cctggtaggc aaacgcgaag tgctcggcac ctctgccccg ctctacctgt atggctacgg
     3121 cgcttatggc gaaagcctcg acccctggtt ctcccacgcc cgactgtcgt tgttggagcg
     3181 cggttttatc ttcgccatcg cccatgtacg cggtggcggt gaactgggcg aagcctggta
     3241 tcgcgctggc aagctggagc acaagcagaa ctcgttcagc gactttatcg cctgcgccga
     3301 acatctgatc gccagcggct acaccagcgc cgagcagctg gtgatcagcg gcggcagcgc
     3361 gggcggtttg ctgatgggcg cagtgctcaa tcagcgtccc gagcttttcg ccgccgccat
     3421 cgccgaggtg ccgtttgtcg atgtgctgaa caccatgcag aaccccgacc tgccgctgac
     3481 cgtcaccgag tacgacgaat ggggcgaccc aaaccagccc gaggtattcg agcgaatcaa
     3541 agcctacgcg ccttatgaga acgtgcaggt ccaggcctac ccggcaattc tcgcggtggc
     3601 cggctacaac gacagccgcg tgcagtactg ggaggccgcc aaatgggtgg ccaagctgcg
     3661 cgcgcagaaa accgacaaca atctgttgct gctgaaaacc gaactagacg ccggccacgg
     3721 cggtatgagc ggccgctatc aagctctcaa ggatgccgcc ctggaatacg cctttatcct
     3781 caaggtactc ggcctggttt gagtccaact cgccgctctg ccgcccgctc ttatgtgggc
     3841 taacagggcg gcgcaggctc agagtaattc gcgctaaaca gcggacgttt cgggcgaaaa
     3901 attacttgac cgtttatgca gatttaacct gactgacgtg caagttcggc taggctgaat
     3961 gacacgctgg cgtactgcca tctcgcccat tcaactgcct gctcaccatg gacggtctgt
     4021 gatgaaaaac tggaagatca gaacccacct attccttctc tctggctgcc tgctgtttgg
     4081 gttgttgtgt gtcggcggcc ttggcctgta cggcctgcag gccacagtca agggactgca
     4141 aacggtgtat ctggaccgcg tggtgccgtt gcgcgacctc aaactgattg ccgacttgta
     4201 cgccgtcaat atcgtcgatg cgacgcacaa ggcgcgcagc ggcgagctgg atgaaaacac
     4261 cgcgcaacag ctgatcgagc aggcccaggt gcggatcgag gaaacctggc aggcttacct
     4321 ggccaccgaa ctgatccgcg aagaaagcca gctgatcgag cagatcaacc cgctgatgca
     4381 acaggcccgg caaccgttga atgagctgca aagcctgctc aaccgtggcg ataaagtcgg
     4441 cgttgaagcc tttgccagcc ggcggctcta tccattgatc gatccgctct ctgagaaatt
     4501 cgccgagctg atcgacgtgc agctgctcga agccaagcac caataccagc tgggcgaagc
     4561 cgcctatgcc agcaacctca acctgagcat tagcgtgctg ctgattgccc tgctggttgg
     4621 tctggcacag gtgttctatt tcattcgcct gatgagccgg caactgggtg ccgaacccgg
     4681 tgagctggaa gaaatcagcg cgcacatcgc cgaaggccgc ctcggcggaa aaaccttggg
     4741 caaccaggtg atgaccggcg tgatgaaatc cgtgcagagc atgcgccgtg gcctgcagga
     4801 catgatcggc aatatcggcg aagcctccga gcagatcgaa tgcgccaccc tgcaactggc
     4861 cgcttcctcc gagcaggtgc tgaccagcgc caacgtgcag agcgacaccg cctcggccat
     4921 ggccgccacc gtcgagcaac tggcggtgag catcaaccac attgccgaaa acgcccagca
     4981 aacctacgac atggcgaaga aagccggtga tatgaccgac gcaggcattc aggtgatggc
     5041 ccgcgccacc acagaaatgc acaagatcgc cgagctggtg gccgagagtt cgcaggatgt
     5101 ggaaaccctg gccgcgcaat cacgcaatat cagcgcgatt gtcgacgtga ttcgcggcat
     5161 cgccgaacaa accaacctgc tcgctctcaa cgcggctatc gaagccgcgc gtgctggcga
     5221 gcaaggccgt ggctttgccg tggtggccga tgaagtgcgc agtttggccg ggcgcactgc
     5281 tcagtccacc accgagatca tcgccttggt cgatgcgatt cacagcggta tgcacaaggc
     5341 aaaaagcagc atggctgccg gctgcgaacg ggtcagcatt ggcacccagc tggtcgatca
     5401 ggcgggcagc tcgatgacgg ggattcgcct ggcgctgaat gaatcactcc aggccgtcag
     5461 cgtaatctcg ctgtcgttgc aggagcaacg cgcagccagc gaacaggtgg ccatgagcgt
     5521 ggagcgggtg gcgcaaatcg tcgaagaaaa ctccgccgcg caaggcggca tcgtccaggc
     5581 catccagtcc ctgcaagcca tgtctgggcg tttgcaggga gtgatgcggc gcttcagttt
     5641 ctagatcgta gatagccgtt ccggccactc gacagtaggt cgcaagtggc gctggcgtat
     5701 gaccgatggg cagctggtcg agcagcagct ctagctaccc gcaattcact catcgggttt
     5761 cggcagcggc tgcgcattgc gttcacgctg ttctcgcagc aacggcagtt cctgatcgct
     5821 gcgtggctgg ctggactgcg gcggcgatgg ctgcttgagc aacggcgggt tattgcggga
     5881 cggggcaggc agatgctgcg gcgcagtcga cggatagggt tgcggcgtgg cggtgcctgg
     5941 ggcacctggc gcaggcgaag tagcgggcat tttcggcggt tgctgagcca gcagcgaggt
     6001 gctggccagt gtggtgagga caagcaacag agtcgtcagg cgcatgcaaa tatctccata
     6061 cagttactca cttgggcctc agcaaggctg ttaagtgccg cacgggcggt tctcactgac
     6121 cgccggcaag gctagactga tcgcacagcc ccagcgtacg agattccaga tgagcagcga
     6181 caaatccccc tccgccaccg gcaagcttga ccgcatcctc gccgaggccc agcgctcgcg
     6241 tgaagaaggc taccgcgaca aggcgctgaa gatgtacccg cacgtctgcg gccgctgcgc
     6301 tcgcgagttc tccggcaagc gcctgagcga gctgaccgtg caccaccgcg accacaacca
     6361 cgacaacaac ccgcaggacg gctccaactg ggagctgttg tgcctgtact gccatgacaa
     6421 cgaacactcg cgctacaccg accagcaata cttcagcgaa ggctcgctga gcacaccgaa
     6481 gactgctcag gcgacccaca accccttcgc cgacttggct gccaggatga aaaagcagta
     6541 gcgcaagcct ctagaaacct cgcttgctga cgatctaagg gttagtcgca tcgccagcaa
     6601 gctggctcct acaaaaatca gccggttgcc atacaggtat actcgcgtct tttttgtcgc
     6661 ccgccatcct tggcgcggcg tcaaaaacat cctcaaaaat tctgtgcgag tgccaccgtg
     6721 gccaacaaga gatacgcctg catcggcctg ttcaacccca aatccccgga aaacgtcggc
     6781 tcagtgatgc gcgcagccgg ctgctatagc gccgcctcgg tgttctacag cggtaaacgc
     6841 tatgcccgtg cccgcgactt tattaccgac acccagcgca tttatatgga tattccgctg
     6901 attggcgtgg aagacctgca gcagatcatt cccatcggct gtacgccggt cgccgtggaa
     6961 ctggtcgaag gtgcgcggcc actgccgcaa tatacccacc ccgaccgcgc catctatatc
     7021 ttcggccctg aagacggctc gctgagcaag gaagtcctcg actggtgcga ggacgtgatc
     7081 tacatcccca ctcaaggctg tatgaacctc gccgccaccg tcaatgtggt gctctacgac
     7141 cgcatggcca aaggtatcaa caccaagtcc gggcctgggc tcaagtaggc tgcagcctag
     7201 ttgttacaac cctgacttga gccgactgaa agcgcgaggc agtgcgcggt tgaaggcctg
     7261 actgttgtca tttccggtgt acagctgggc gcgcccagcc gcagcccggc caaagtgggc
     7321 gtaccgggcg gcgcactcga aggtgcgcaa ggcaatgaga gacggtaatg cgagtcccag
     7381 ccccgggcta gcgcaacagc ttgttgtcca gcgtcttcga ggcgctgccc atgacgtttt
     7441 cgctgatctc gataaagtcc ttggtgctag ccttgtcgag gcgcatcagg ccgcgggtca
     7501 cgtcatccag cgagcgtggc tgggtggtgc ctttggtggc ctggcggatt tcccgatcca
     7561 gttcctgcag cagcaacacc gcacgggcgg tgaccgggcc gctggagttg ttggtgcgca
     7621 ggctgtccac cggcttgctc caacggatca gctgctcgcg gatcttctgg tagcgctcct
     7681 cgctgaggcc accggcgcgg cgcatcagct cgatggcgta atactcggcc aggccttcgg
     7741 tgatccagtc gctgcgatca cgtgccttga ttcggctgaa tacatgcacc agctcatgca
     7801 ccagggagct ggtgccgttc tcgctgacca gcggtcggtc cgtgtgcatg tagtaggagt
     7861 tggccgccga caggccgccg cgccacatcg ggtcaccggc gccgacgatc agcagcttgg
     7921 ccgggtcgcg cggaaacacg tcctgggcat gcggccagat aaaggtcagc agggtcatca
     7981 cgtccatgcg gcgcatgccc tcgcccactg gcgcggcgat ggccacatcg gtttccccga
     8041 gtttggcgcg gcgcgtgccc accttgccgg ccaccatcca gccagtcgga cggtcaaact
     8101 tgcgctgtgg gttgtcgatg cggaacttgt tcttgccgat acgcggccag ccagtttcaa
     8161 cgctcttcca gcccttaggc agatcgaatt gcagacgcga gaccagctcg gttttatctt
     8221 cctgacgcag cttgaccgcc ggcaccaggt cgtcgccgcg cagcaaagcc cagtccttgt
     8281 tgatcaacgc atcaaaacgc ccagcgctgc gctcattgtt gatacgcacg cggtaagtca
     8341 gagtcgattt acctttgccc ggctgccagg tacccagctc ggcgctgttc tgcgtccact
     8401 ggccgtcggc cttgaagtcg ctataggcac cctgctcacc caggttgaag gccaagctga
     8461 tcaccggcgc gccgtcttcc agaatcaggc tgacttcagc ctcttcggtg tccggcagga
     8521 agcgcacgtg ataatccaga tcgaccttct tcgcccacag cggcgtgctc aggctcagca
     8581 aggcggtagc cagcaacaga cggcgaatca gcataagaaa agctccatga ggcagtcatt
     8641 gatccagacg agtttaggct gaggttgccg ggcaaggttc aaaccgattg cagggccact
     8701 cagcctgcgc ggaagatcat gtgatcttcc cagtcatctt cggccacgct gttttcactg
     8761 agcatgcgcc cagcctggga aatacgctgc ttgtgcacct gctccgggtc gccgcatacc
     8821 agatggtgcc agagcagcag atccttgccc tcactgacca ggcgataggc gcaggtcggt
     8881 ggcagccact gaaactggtc ggcctgggcc ggggtgagct ggatgcagtc ggcgacgaat
     8941 ttgcgccggt tgctgtagtc ggtgcagcgg caggtctgca ggtccagcag tttgcaggcg
     9001 atgcgcgtgt aataaacgct gccatcgtct tcatcctcaa gcttttgcag gcagcacagg
     9061 ccgcagccat cgcacagcga ttcccactcg tcctgatcca gctgttcgag ggttttgcgt
     9121 ttccagaagg gttcgacttt ggcggccatg gcgagggtac agtgaggggc gaaaaggccg
     9181 gcagtctagc ggctggggca acaacggcca agggcggcaa gccgaatggt taattaataa
     9241 ccacgaacaa agtccacatg cccgcgcaga gcagaacctg cctgccagcg caccaggtta
     9301 tcgacaaaca acttcaccaa tagatgagga tcagtcggag ctgagctatg cccagtcagc
     9361 aacagacgcg gtgcatccca gaatgggtgg ccagcgggca gcggttcttc acggcagaca
     9421 tcgatcaccg cgccagccag ttgtccctgt tgcaaggcac tgaccagcgc ctgatcgacc
     9481 accgccacgc cgcgcccggc gttgataaac aacgcgctgg gcttgcaggc ggcgaatagc
     9541 gccgcgtcat agatatcgcg ggtggccggg gtatccggta gcaggttgat caggtaatcg
     9601 gcctcgccca ccaggcttgg cagctcgtcg agccccgcca cctgacgaaa cggcggcaga
     9661 ttgcgcgggc tgctggcgat gcccagtaaa tcgatgccga acggcgccag aaactgcgcc
     9721 acgctcaagc caatatcacc cgtaccgacg atcagcgcct tgcgcccggc caggctgcac
     9781 ggcagacgat tgtcccagcg ctgctcgacc tgcgcggcca ggcgcgacag caactggcgc
     9841 tcgtgggcca gcagatggca cagcacatac tcggccatca cctggccgaa gatgcccacg
     9901 gcgcgggtta acgcgtagtt agtcggcaag tcacccgcca gcagcggcgt gatgcccgcc
     9961 caggtcgatt gcagccactg cggccggctg ccctgacgca gcaagggcgc gagcagatcc
    10021 ggctggccca gccataacgg gtagctcgcg gcgtattcgg ccaactcggc cggccgatcg
    10081 ctgccaaaca gctcaagctc aggtgcctgc tcgcgcagta actcggcata cagcgcgtgc
    10141 tcggcttcgg caatcaatac acgtacaggg gcattcgcag tggcgggcat ggcggttaca
    10201 tcgggtcgtt acggcgcaac aactcatcgg gcagatgctc gatgtattca tcttccggcg
    10261 gcggcatctg caggtggtag ccctgctcgt cgaggttggc cagcaccttg tggatgtctt
    10321 cgcgggccag gacgcgctcg ggggtcagca ccatctcaaa ggccagggcc ggcgggccga
    10381 aggccagcag caggttttcc ggcacgcgct tgagaccatc gctgcgcagc acatagagat
    10441 acatctcgct cttgcgcggg cttttgtaga tcgagcagat acgtttcacg cggactctcc
    10501 ggttgcagcc aggcaatcaa gcagcgcctg gcccattaat tcacgccgcc agccgcgcag
    10561 cgactccggc agctggtaag gcccattcgg atagccggtt ttcagcaggg cttcgagggt
    10621 tttcttgcgc agcatcagct caggggcaat gttcaggcgc tcaccttcac gctggccaat
    10681 ggcgcgcagc tttttcagca gcgatgcagc gtccagcggc aagggctcag gcaacgcctc
    10741 gggccaatct tcagccgggg tggcagccgc ctgcttgatc agttgcaaca gaaactcgcc
    10801 atcctggcgc acggttttcg ggtgcatgtc ctcgatccgc gccagggcca ccaggttatc
    10861 cggctggctg cgcgccaatg gccacagcga atgctcacgg ataatccggt tacgcggctg
    10921 attgcgcttg cgcgcctggc cttcacgcca ggtacacagc gctttcagca cggccaactg
    10981 ctgccggttg agtttccagg ccagtttggc ttcgcggtag gccagttccg gatcgacttc
    11041 acgcttgagg ttgctgacca gctcggcacc gtcttccagc acccaggcgt atttctcggc
    11101 agacagctgc gccttgagct gcgcatacac ctcggccagg tgcagcacat cttcagctgc
    11161 atagctgacc tgagtggccg acaacggccg ctgcagccag tcagagcggg tttcgccctt
    11221 gggcagctcg atattcagca ccgcctgcac caggcgtgaa tagcccatgg agaagccgag
    11281 attgaggtaa ccagctgcca gctgggtgtc gaacagcggc gtgggcaggc tgccggtcag
    11341 acgcagaaag acttccaggt cctcgctgca ggcatgcagc accttgacca cggccgagtc
    11401 ctgcaacagc tcggcaaagg gcgcccagtc gctgatcagc agcggatcga tcaggtatgc
    11461 ccgctcgccc tcgctcacct gcagcaggcc ggcaatagga taaaaggtgt cgacccgcat
    11521 aaactcagta tcgaccgcca caaatggcag actgcgccag gttgcgcaat gctgcgccag
    11581 gctggcgtta tcaccaatcc actgaatatc aatcgccaca cagctctccc atcaagaatg
    11641 ccgcgcagta tatatgccca ggccctgtgc cgggcatttg ccggccattt acccccggga
    11701 cctcacagat gcgaatgaac cctgtaatct ggccggcgct tgcatctgct taccttcatc
    11761 tcagggaata aaaacaatga agaaactgct tggcctgctt gtggccttga ttgctgcggc
    11821 ggcgatctac ctcgccgtca cacccagccc gattgatccg ctggcctggg aagccccggc
    11881 catcccgcca atgaccggcg tactggaaca caacgacacg ctgatgaagg ccgaactgct
    11941 cggccgtggc caggtgcatg gcccggaaga caccgcagtg gatacccagg gcctggtgta
    12001 tgccggcctg catgacggca agatcgtgcg catcaaagcc gatggcagcg tggaaacctt
    12061 tgtcgacacc aaaggccgcc cactgggcat ggactttgac gccgccggca acctgatcgt
    12121 cgccgacgcc tacaagggct tgctgagcat cgacgcccag gggcagatca aggtactgac
    12181 caccgaagcc gaaggcgtag ccttcaagtt caccgacgat ctggatatcg cacgcgacgg
    12241 caccatctac ttcagcgatg ccagccagcg tttcgaacag ccggattacc tgctcgacct
    12301 gctcgaagcc cgcccacacg gccgcctgct cagctacaac ccggctactg gcgaaaccaa
    12361 ggtactgctc aaggacctgt acttcgccaa tggcgtggcg ctgtcggcgg ccgaggactt
    12421 cgtgctggtc aacgaaacct accgctaccg catcacccgt tactggctga gcggcgacaa
    12481 ggccggtacc cacgacatct ttatcgacaa cctgccgggc ctgccggaca acctgcaggg
    12541 cgaccgcaac ggcaccttct gggtggccct gccgaccccg cgcaaagccg atgccgacat
    12601 gctgcaccgc tcgccctggg caaaatcgca gctagccaaa ctaccgcgcg ccttctggcc
    12661 caaggccgtg ccttacggct ttgccatcgc cctcaacgag caaggcgaaa tcacccagag
    12721 cctgcacgac accagcggca cccacctgcg catgattacc tcggtcaagc cggtgggcga
    12781 ctatctgtac tttggcagcc tggataatga ccgcattggc aaactaaaga ttcgctaagc
    12841 aacccacaca aaaaagcccg cgcaactgct tagctgcgcg ggcttttgaa tattcgggat
    12901 ttgcctattt gatcttttcc gcgatccaga tggtatggcg ggtgcccttg ttgccatggg
    12961 cgtagacctg cacctcctcc accttgaagc cggccttgcg caccttgtcg ctgaataccc
    13021 ggtcggcact ggctgaccat accgccaaca cgcccttgtt acgcagcgcc tggtggcagc
    13081 gttgcaggcc ctgggaggaa tacagccagc tattgcgttt ctgggtcagg ccttcagggc
    13141 cgttgtccac gtcgagcatg attgcgtcgt agcgctgatc ggcactcttc aggcaattgg
    13201 cgacatcgtc ctgaatcacc cgggtacgcg gatcgaggat cggataaccg gccttggcgc
    13261 ccaacggacc gcggttccac tccaccaccc caggcactag ctcggccact tccacttcag
    13321 cggtcttgcc caggttctgc aacgcagccg ccagggtaaa gcccatgccc aggccgccga
    13381 tcagtacccg tgaatccggc cggccggcga ccttcttgca gggaatcgcc gccagggcat
    13441 cttcagaacc atgcatgcgc gtattcatca gctgaccgcc atcgccgccg gcaatcttga
    13501 tcacaaaatc ctcaccgtac tcgaacaggc acagggcacc cgagccatcg ggaatcgggg
    13561 cggtatcgag caatacaaaa cgcttcatgg ggtgcggcaa cctgggtcag aacaaaacgg
    13621 gcatgcagca tgcccataaa ctggcaaaga tgcagcgccc tgtcggcaga aattcttctg
    13681 tacgcggcgt ctcgacagcc caacgccagg gtttatgatg ccgactgcac tagaatctgt
    13741 tcaggatctc gcgagctaga gtcatgcaag gcaaaaacag gcgaggaagc ggagtgtact
    13801 tttgtacatg agcattcctc gcctgttttt aacgcagcag ggccgacgcg cagcagatcg
    13861 tgagcagatt cttatttacc cacaggatgg actgcatgcc cctcgcccaa ctgatcagtg
    13921 ccgcccagct gcagcgccgc cttgatgagc ccgacctgct gattctcgat tgccgctttg
    13981 ccctggatga tctcacctac ggccagcgca gctacaccga agggcacatc cccggcgcgc
    14041 agtttgccga tctggagcgc gacctctcag ggccgatcca caaaggcatc accggccgtc
    14101 atcccctgcc ggagcctgct caactgctgc aacgcctgcg cgaatggggc ctaaacaatc
    14161 acagcgaagt ggtgctgtac gacgacggcc ccggcgcctt tgccagccgc gcctggtggc
    14221 tgctggcctg gctgggcaaa cgtgagtgcg tgtacctgct cgatggcggc ctcaaggcct
    14281 ggcgcgaagc cggtggggca ctgactcagg acctgcccgc acaggctgcc ggcaccttca
    14341 gcggcaacgc cgatgccagc ctgctggtta gcgccgcgca gctgcaacag cgcctcggtg
    14401 atagccaact gaccctgctc gacgcgcgcg gcctgccgcg cttcagaggt gaggtagaac
    14461 caatcgaccc ggtcgccggg catatccctg gtgcgcagtg cgcagcgttc acggacaacc
    14521 tcgacagcga cggccacttc ctcagcccag agcagctgcg ccagcgcttt gccgcgcaac
    14581 tcggtgagcg cccggccagt gaactggtcg cttactgcgg ctcgggcgta accgcctgcc
    14641 ataacctgtt cgccctgtgc ctggccggct acccactggc gccgctgtat gccggctcct
    14701 ggagcgaatg gatcactgac cctgctcgcc cggtggctac tggcggctga tttcagctcg
    14761 tgcaggctgg ccgtgcgggt atcgcccgcc agccgtggtt aagcaccaac tggaatatag
    14821 gcaataaccg aaagccgaaa acaatttaaa aacagccctt tacagttgaa aggtcgcact
    14881 acacttcaac atgagcggct aaccccgctc ccggtggggc ctttatcaga ataagtcgaa
    14941 gctgccagcg ccccggtccc atcggtcatt taccgccgag ggctttatgg gaattgccgc
    15001 ctgcgagtta agccagtacg tcatccgccc taccctgctc tacctgggcc gccattccaa
    15061 cgccgccgaa gccttgttgc tcggtgccgc cgccagccaa tccgggctcg gttcggccct
    15121 cgatgacaaa catggtcatg gcctttaccg catcgccgca cagcgccacc agcagctctg
    15181 ggatgagcat ctggcgctgg acccggatct cgccagtcag gtacgcggcc tcgccagcca
    15241 gcacgccttc ctcgccgctc cgcacctcga actgaccgtc aacctgcgct acgccacggc
    15301 cattgcctgg atgctgattg aagccgaaca ccgcgaactg ccggccgccg atgacctgct
    15361 cggcatggcg caagtctggc gccgggtatt caggccacac ggccgcacca aggactttat
    15421 cgccgcctgg caacgctgca tcggcagcct cagccaagcg gcctgaaacc cggataaacg
    15481 ccaaacattg tccgacaact tcagcgtagt tagcaggcaa tagcgccacg ttaaaagcat
    15541 atagcgctct atctcgggaa cttcacaggc ttggcaaggg tcaggaaaag ttgttagttt
    15601 ccgcccccgc taaaatctag gagtcctccg tgactagaag agtcgtcaag acctgcctgt
    15661 cccttgccct tgcctctgcc gccagccatg gctttgccag tggttttgcc atcaacgaac
    15721 aaagcgtcag cagcatgggc accgccttcg ccggccgctc ctcctctgcc gatgacgcca
    15781 gcaccgtatt cggcaacccg gccggcatgg cgcgtatcaa gcgcgaacaa gtcagcggcg
    15841 gcattgcgct gatccacgca aaaaccgaga tcgacaacgc cagcggctct gtcagcggta
    15901 gcaatgacgg cgacatggtg ccgtttatcg gcgcgcccat gggctattac gtcaaaccgc
    15961 tggacgacaa ctggagcttt ggcctgggtc tgtatgtgcc gttcggcctg gtgaccgatt
    16021 acgaacgcaa cttccagggc cgctatcacg gcgaccgtag cgaagtgcgg gtgattaccc
    16081 tgcaaccgac cctgagctat cgctttaacg agcacctgtc gatcggcttc ggcccaacca
    16141 tcaaccgtat cgacggcgag ctgacttcag ccatcaacaa cccgatcccc ggcgcaggtg
    16201 acggcaaggt caaaatcaag ggcgatgaca ccgcgctggg ctataacgtc ggcgtgctgt
    16261 ttgaaatcac cccacagacc cgtttcggca tgacctacca ctccaaggtg gattaccgcc
    16321 tgaagggtga caccacggtt tccggcccgg gcttcgtcat tggctcttcc gctggcacct
    16381 atgatgcttc actggagctg accaccccgg aatcggtgga tttctcggta actcacgaac
    16441 tggatgccga ctggactcta tacgccggca gcacctggac gcgctggagc cgtctgcaag
    16501 agattcgtgc tgataacaaa ggcgtaggtg gcttgctcgc aggaaacctc agcagcatca
    16561 gtgaaccaca gaactggcac gacacctggg cgcacgccgt gggcgctgcc tacaagctga
    16621 atccgcagtg gaccctgcgc gccggcctgg ccgtagacca gtcgccgacc aataacgccg
    16681 accgttcgcc acgcatcccc accggcgacc gcaccatcct cagcctgggc acagcctgga
    16741 gcccgaatga tgatgtgacc atcgacctgg cctactccta cctgatggaa gacgacgccc
    16801 atatcgaaag ggacgattac cgcgcaacct acaaaaacac ggcccacggc ctgggtacac
    16861 aagtgacgta ccgcttctaa atcaaccagc ggtttgggtt aaactcacgc ggcgtccaca
    16921 aaaataataa cgccgcctga gacagcccta tgactgcact ctgcactgcc ctgcccgaac
    16981 agctcagcct gttactggta gatgaccacc ggatctttct cgatggcctt agtctggccc
    17041 tcgccccgct ctgcgccgac ctgcagattc gcaccgccga atccgcagcc gccgccgaag
    17101 cctgcctgca acacgccacc ttcgacctga ttctgctcga tctgcgcctg cccgatatgc
    17161 cgggcctgga gttgctgcaa cgctggcaac aacaaggccg catgacccca gtagcgatcc
    17221 ttagcgccag cgattccaac ctggatgccc aagccgcact ggccgccggc gcgctgggct
    17281 ttatcccgaa aagtgccaat agcgatgaac tgcgccaggc agtaacccgc gtactgctgg
    17341 gagaaaccct ccccgcaccg caaactggcg acaaaccgca gctcacccct cggcagatgg
    17401 aaatcctcca gctactcgcc gaaggcttgc cgaacaagtc catcagccgt cagctgggcg
    17461 tttccgaaga cacggtgaaa acccatctga aatcactgtt tcaggagttt tccgtacata
    17521 cccggactgc ctgcgtcagt gcggcgcggc aacggggctg gctttaagct ctgcgggcaa
    17581 cagcaccgcc agagcctgac gtaaacgggc tggcagcagc ggcttgccga gcaaggtgac
    17641 atgggcgcgg gtgcagcgct cctgcaggtc agcgctgacg tcggcggtaa tcagcaaggc
    17701 cggcagcatg cgcccggcct cttcgcgcaa acgctcgata acgcgcaagc cgtcctcggt
    17761 ggcggccagg cggtagtcgc tgatcagcac ctgcggctgc gcctcgggca aacactgcaa
    17821 ggcctcggct ggatcggcgc aggcccagac ctcgcacccc cagcgctgca gcaaaccgga
    17881 cagcgcctcc cggcctgcgg catcgtcttc cagcaataac acccgtccgc tcaatgccgc
    17941 ggcaacggcc gctggtgctt gccactcgcc ggcttcagcc tgcggcagtt gcaggataaa
    18001 gcgcgcgccc gctcctgggc tcgactccag ctgcagcgga tgccccagca gctgcgccag
    18061 ctgctggacg atggccaagc ccaggcccag accgcgctcg gaattacgcc ccgggttatc
    18121 cagttggcgg aactcttcaa agacctgact ctgctcctcc tcactcaggc cctggccgtc
    18181 atcaccgacc tccagcagca ccacgccctg ctcgacacgg gcatgcagcc agacatgccg
    18241 cgcctgggca tggcgcaagg cgttgctcag caggttgcgc agcacgcgct ccagcagcag
    18301 cgggtcactg cgtacccaga ggtccgcgca ttgcagctgc agctcgacac cctgggcgtc
    18361 ggcctcggcg cggctttcac cgctcaggcg cgccagcaag gggcgcaagg caaacacttc
    18421 cggcttggcc tgcacaccac caggcagggt cagacgggtg taatcaaaca gcgactgcaa
    18481 cagccggctg agctgctcca cggcaatcgc caggcgactg ctgatgcggt gcacgccagg
    18541 gtcctggttc tgctccagca ggtgttgggc atacagaccc atggcgttca gcggttggcg
    18601 cagatcatgg ctggcggcgg ccagcaggcg cagcttacgc gcatcgtcgg cttcggcgcg
    18661 ctgccgcgcc agatcgagca aacgcagatt gagccaggcg ccgcgctggc cgtgctcctg
    18721 caggtaggcg ccaacactgc caatcaggtt ggcgcaaaac agaaacaagc cctgataacc
    18781 cagcgccacg ctgccccccg tagcgatcag ccctagccag aggaacagcc cgcacacacc
    18841 ccaggacgcc aggctgacgg agcggaacga cacccctaac agcacgtagc caaagagcaa
    18901 caccagaaac agcccgtcat agggcatggc tacgcccagg ctccagcaca gatgaatgat
    18961 cactgcgacg caaacaccgt tgagggcata agcggccaca tagaccagca aggcgcgccg
    19021 cggcgggctc tgggggcggc ggcagtagaa gaaggacagt agcgcaccga atatgcccag
    19081 cattcgcact ggcaaggtgg taaggctggc gttgagaggg agcagcaata aatcgaggac
    19141 ggtgtagacc agctgaaaga ccaccgcagt gctgatgatc agcagcaggc ggcggcgaat
    19201 acgttgcacc cggtgctcga caaactcatc ttccagcaca tcagtaaaac gcaaccggcg
    19261 atagccgcgg cgctgctggg ccagtagcag cgcttcctga cgctccaggc tcatgaatta
    19321 caactcaggt gacgagctgt cctgctgctt ggcggcaatg gcgtcatagg tttccacccg
    19381 gtgctgatct tcggcgctga atagacgctg gcgcaaacgc tgctgctcct gctgctgatc
    19441 cgcagcactg aggccattgc cctgcaaggc ggcaagctcc tgctggtaag tggcatagcg
    19501 ttgttgccag gcgcgctcgt tgcgctgctc gctgagcaga cgttcgaccg tgggcggatc
    19561 ataggtcaag gcaagcaact ggcgcacctg ctcttcgctt gccccctcct gccaaagctt
    19621 gtctgtctgg acctgcaacg cctgggcttg agcctgacgc tcttcgctgg cgcgcatggc
    19681 ttcggggagt tgagcgcgca aacgctcctg cgccagggcc ttgccgtgct cgctcagatc
    19741 gtcacgggcc atcagcgcca ggttatccag ggtgtaacgg ccgtaggctt cctcggcgcc
    19801 gaacagggcc tcggtcgccg cgctggagaa atgcgtccga cgcagctgcg ctaactgctc
    19861 gaaggcccgc tgcaaggcaa ccagctggcc atccggggtg ctttgctgag cagcgctcaa
    19921 gggttgctgc agcaacgcca ggctggcgcg cttgtaatcc atatagtcgc ccagcaaacc
    19981 aataaactgg cccagggcgg gctccggcaa acgtgtgcgt gcatcggcca gcatggcctc
    20041 caccacggtt tccaacccgg cctgatcggc ggcgctgagg aagtaatcca ggtaatcgcg
    20101 cagcgccagg ttcagctgca gattgccggc gctgtccgcc ttcagctcgc catccacgtc
    20161 ggtaccttgc aaacccggca ggtgtgccgg cgagccagct gcagcggtat ctggctgggc
    20221 gactggcaat agagggctcg gtgcgacggt aagaggagcc gtggcggtaa ccactgcagc
    20281 aggcgcgacg gctacagctg tggctggcca ttgccaataa agcgtaaggc cggcaacgct
    20341 gccggccagt aacaaaagag ggatggttga gcgtaacgac agggccatat gacctccaga
    20401 atgcgggctg ccttgcgcag cccgccggtg cgaaggtcag acgcctttgt ttttcaggcg
    20461 gttggcatgc tggcgataca gcgaaaccgg ctcggtccag ccacgaatac cgaacaggtg
    20521 gttgatcgca tccacgtggt tcatgttgta gccatcgccg atcacctgcc ccagataggt
    20581 ggagcagacg ccaaccaggc catcattctt ctcgctacca aaggctagcc cggtgatcgc
    20641 caggaacgga tcgaccgcat caaagacgtt ggtgtaggag gtattaccgg tccaggagaa
    20701 gtaacggatg ttgtgcccac gcacgttgtg cacttcggtg gatttggcgc agtagctgct
    20761 gctgtttacg ccccacggat ggcgaccgtt cagcgcagtc gtaccggcag tggtgagggt
    20821 tttcagcgca tcgataccgc tctgcggatt gctgctgccg gacagcaggt tgattaccgc
    20881 acccagggcg ttggccaggg cattggcacc accttcgatg gcgctgttcg gcgggatcac
    20941 gccacgcagt acgtcggcga ctttcgagcc tttgttaacg ccgttgaccg aggtcaccga
    21001 ggccaccaaa tccgggcgca gcgaggccgc gacgcgcgag gttggcgagc cctggctatg
    21061 gccgatcagg ttgaccttgc cgccattggc accggcccag ggcacgatct gctgcgccag
    21121 tgcggcaccg cgttgttcgc tgtcattaag tgccgaaacg ctggccacat agaccttggc
    21181 gccatcgcgc tcgaggttcc aggggatggt gtggaagtag ttgatcagac caccgatggt
    21241 gttgaaaccg gttacgccgt gtaccagcac gatcgggtat ttggtcttgg tgtagttggc
    21301 ctgagcctca accgcgccga ccagggcaac ggcagctaga gcagtagtaa taacgcgacg
    21361 catgggtgcc tccttatttt tcttgttggc acgccgggaa actgcgtccg gcgtactcac
    21421 aactctaagg aggtgcgggg tggtgggcta tcgcccagat gggtgatgca tcaaggctgc
    21481 gaggcgatgg ctttctcgac ggcagccagc agcgcagggt catccggggt ggtgctgctg
    21541 gggaagctgg cgatcaccct gccctgggcg tcgatcacat acttggaaaa attccagcgt
    21601 ggcgcctggc tttgcgcggc cagctccttg aacagcggca gcgcatcgct gccggtaacc
    21661 cgctgggttt cactcatggg gaaggtcacg ccgtaattga cgtagcagac cttggcggtt
    21721 tcttcgctgc tgtcggcctc ctggcggaag tcatcggaag gcacaccgag gatttgcagg
    21781 cccagctcct tgtagcgctg gtagagcgct tcaaggcctt tgaactgcgg ggtaaagccg
    21841 cagaagctgg cggtattgac cactatcagc ggcttgccgg caaactgcgc gcacaggtcg
    21901 atgcgttcct cgctgcgcaa cttctgcact tcgccctgca gcaacgccgg gcattcggcg
    21961 gcttgcgcgg gaccaacccc ggcactaagc agaacgacaa ccaacaggga aaaacgccac
    22021 atacggatca ccttgatgag atcagcaaac acccagcccc agctgtaaga atgcgaggcc
    22081 actttgcagc cagccccacc aggccaggcc caaggccaac atgccgccca tcaccagcgc
    22141 ggtgttccag ccatagtcgg tattcatgca ttcgcctcct gttgttgcaa gcgtgccacg
    22201 ggcagctcac gtactggcca gttcaacgcg gcggccagca ggctgagcag cacggcaatc
    22261 tgccagacca gatcataact gccggtgtgg tcatacagat agccgcccat ccaaccgccg
    22321 agaaacgccc ccagctgatg gaacagaaag acgatgccgc caagcatcga caggttgcgc
    22381 acgccgaaca tggtcgccac agtgccgttg gtcaatggca ccgtcgacag ccagagcagg
    22441 cccatggcca caccaaaggc ataggcgctc cagaccgtca gcggcgtcat aagaaacgcc
    22501 acaatcacca ccgcccgcgc cagatacaaa gcgctgagca ggcgcggctt ggacatccgc
    22561 ccgcccagcc aaccagcaat ataggtgccg aaaatattga acaaaccgac cagggcaagc
    22621 accgtggtgc cgaccagagc gggcagatgc tgatcgacca gataggccgg caaatgcacg
    22681 ccgataaaca ccacctgaaa gccgcaaaca aaaaagccca atgccaacag ccagaaaccg
    22741 gaatggctgc aggcctcgcg cagcgcttca ccgagggtct gctcctggcc ggtaagcggt
    22801 agtggcgtgt ccttaagcat tgccgccaac ggtatgatca gcgccaccag caggcccagg
    22861 gccatcagcg ccaccgacca accgagccag ctaagcaggc ccagggtgcc tggaagcatg
    22921 acgaactggc caaaggagcc cgccgccgcc gcgatgccca tggccatgct gcgtttctcc
    22981 atcggcacgg cgcggcccac tgcaccaaga atcaccgaga acgaggtgcc agacaaaccg
    23041 ataccaatca gcagcccagc gctcagcgac aggcttaggg gcgaatcggc gaggcccatg
    23101 cagccgagac caatggcata caggatgccg ccgacaatca ccgtacgctt ggcgccaaaa
    23161 cggtcggcca gcgcgccggt aaacggttgg gtcagacccc agatcaggtt ctgcaaggcg
    23221 atggcgaaag cgaacacctc gcgcccccaa ccgaattcgg cgctcatcgg cggcagaaac
    23281 aggccaaaac catggcgaat gcccaacgac agcgccagga tcagcgatgc accgagcaat
    23341 atccacatac tgccgcgccc aattgtcctc atgctcctgc ctccctgaca gctcaaatag
    23401 tcgttgaatt agttgatcga tcaatcagat cgccgagggt gacgccaggc gagcgttaaa
    23461 acaagtcgac catcgactaa tcaacaaccg tagagccagc acttcagctc ggaaaaaacc
    23521 tgaacacgtc tatcctcgcg tcggtcaaac aaaagtcacc cactattgga gacatgacca
    23581 tgagttcgcc ccaatatgca tcctgcatgc aagcctgcag cgcttgcgca atcgcctgtg
    23641 agacctgtgc gtcggcctgc cttgaggaaa acgacgtaag catgatggcc acgtgcattc
    23701 gactcgaccg cgactgcgcg gacatgtgcc atttagcgca agtgctgatg gcccgcaaaa
    23761 gtgccctggt cgatgaattc tgccgacttt gcgcagtcgc gtgccaggcc tgcgccgaag
    23821 aatgcttgaa gcaccagcac gagcattgcc aacagtgcgc tcaggcctgt cagcgctgtg
    23881 ccgatgaatg caatgcgctg gcgcagtctt aacgctctcg gcttggatac atgcgctaag
    23941 cgctgatagc caaacccagc agtacaacgc attcgagcgt ttccaacaga gcaccggcgg
    24001 tatcgccagt ggtaccgccg atgcgctggc acatcaaccg ccgcacgcag tagaacagcg
    24061 cacaggcgat cagcagcgcc agtagccccg agagcccgcc gcacagcaag caaagcagtg
    24121 cgctggcgat cagcacacgc cagccgagcg cccgtggcag atgctcagcc atagcctgac
    24181 ccaaaccgcc gggccgcaca taggcggtgg tcagaaacag cgccagcatg gcgctgcgcc
    24241 caagcagcgg gaccagcagc aaggcgctaa gcgcagagtt ggctgcaagc agcgtcgcca
    24301 gggcgacaaa cttgagcaac agcagcagca ccagcaccac caccgcaatc ggtccgctgc
    24361 gcgggtcctg catgatcgtc agggtacgtt cgcgatcacc gaaaccgcct gcccaggcat
    24421 cggcgctatc ggccaggcca tcaaggtgca ggccgccgct gagcgccacc cagagcgtca
    24481 gcagcacggc ggcctgcaac attaccgggc gatcacccag catgtagctg gcagcgacca
    24541 gcagcagacc gatcagcagc cccaccagcg gataccagag caatgaacgg ccgatctgct
    24601 gtggcgtcgg cataccgggc aaacggatcg gcaggcgggt gagaaattgc agagcaatca
    24661 gcaatggcgt catgccagtt ctctcaactc gccagcctta tccaggctta ggcgaaagcg
    24721 cgcggcatgc gccacattga tctgtaataa atcgccgcgc ggcaaaccgc gcgcacgcgc
    24781 cagcagcaaa cggatcaccc caccatgggt aaccagcagt aaacgctcgc cgacatggca
    24841 cgcctgcaaa cgctgcaacg cactgaatat ccgcgcctcg aaggccagca gcggctcgcc
    24901 ttcgggcggg gtaaaaccgt agggatcgct ccagaaacgg cccaagtctt ctgcactggt
    24961 ctgcatcagc tccgccgtat tacacccttc ccaggcgccg aaatgcagtt cctgcagatc
    25021 gggctccagg ctaagcggca caccatgttc cgtcgccaac tcttcagcaa agcgcgcaca
    25081 gcgctgcagc ggcgagctga tcagccgatc ccaggggccg gcctcggcca ccgccgcacg
    25141 cagttgcgcc cagccggttt cggtcagggc atcgtcgagg ctgccacgca agccgccgcc
    25201 caattcggtt tcaccgtggc gtagcagttc cagatacagc gtcatgcagg gcgctcggcc
    25261 accgccgcct cggcaaaggt ggccatctcg ttgtgcaggc tgcaggccag ctgcagcagc
    25321 ggcaccgcaa gagcagctcc actgccctca cccaggcgca ggcccagatc gagcaaaggc
    25381 tcggcctgca acgccttgag cacccgctga tggccgggct cggcgccggt gtgggcaaac
    25441 aacagccagt cacggcacgc cggattcagc tgtaccgcca gcaaggcggc aacactgcag
    25501 atataaccat ccaccagggc ggtgatgccc tgctgggcgc aggccagata ggcaccgctc
    25561 agcgcagcaa tctcgaaacc gccgagacgc tgcaagatgt cgatgggttc gctgatcgcc
    25621 ggacgatgca actcaagcgc gatctcgatc acttcggctt tatgcgccac accggcgctg
    25681 tccaggccag tgccggggcc gaccaaatcc tgcgctgggc actccagcag caggcaagcc
    25741 atggcggtgg cggcggtggt attgccgata cccatttcgc cgccgataaa cagctggcta
    25801 ccggcttcac gggcgcgcag cacgctggcc tggccggcct ccagggccag cagtaactgc
    25861 gcggcggtca tcgccgggcc ctgggcgaag ttcgccgtac ccgcgccgat atgcagatga
    25921 cgcacgccac gcagcggttc cagcggcagc gcggtgccca ggtcgatgac ctcaagggtt
    25981 gcgctcagct gcttggccag tacgctgatc gccgcgccgc cgccgacaaa gttgcgcagc
    26041 atctgcccgg taaccgcctg ggggaacgcc gatacgcctt cggcgaccac gccatggtcg
    26101 ccggcaaaga tgctgatcca cagctgctca acctgcggcc gctgcgtacc ctgcatggcc
    26161 gccaggtcga tggccaactg ctccagctgg ccgagagagc cggccggttt ggtcagttca
    26221 agttggcgcg cctgcgcctt gctgcgcacc ggcatatcca gcgcctgaca gccctgttgc
    26281 caccattgct cactcatagc gcattcccct tgagaaccat cggcaaaccc gccacggtaa
    26341 acaccacacg ctcacaacgc tcggccagag cttggtgcag ccagcccgcc tcgtcgacat
    26401 aacggcgggt cagctcgccc agcggcacca cacctaaacc tgtttcattg ctgaccaaca
    26461 gaatccgccc cggcaaaccg ttcacacagt ccagcagcgc ctcgcgctcg gcgcttaggc
    26521 gcgccgggtt atccagcatt aacagattgg tcagccacag ggtcagacaa tcgaccagca
    26581 ggcacttatc cgctgcggcc tgctcacgca gcacccgggc cagtgccaag ggttcttcga
    26641 ccagccccca atgcgccgga cgacgttcgc ggtgatgctg aatacgcgta ttcatctcgc
    26701 catccagcgg ctggctggtg gcgatataag tcactgccaa gccagattcg gctgctaggc
    26761 gttcagccag acggcttttt ccggaacggg caccgccaag aatcagttca tgcatggcct
    26821 actccacata actcgcgcag ccgggccgta tccagatgat gctcgaccag atcggccagg
    26881 cgctcaatat cgcgctcacg cagggcgtgg taatccacct gctgcacctc gcgcagcccg
    26941 gcccagcgca gcagtgcgct gcaggcttcc ggggtttcaa acagaccatg cagataggtg
    27001 ccgagcacct ggccgtcggc gctcaggcca ccatcggcac ggccatcatc caaccgcacg
    27061 accgcattca aacctgggcc agagctgacg ccggcatgaa tctcgtaacc actgaccgga
    27121 gcattttcca ggcacagctg gccgcgcaca ttgcgcagct gcttttcagg ctccagcacg
    27181 gtgctgaact ccaacagacc cagcccaggg cttgaacccg ccgcaccttc caggccatgc
    27241 gggtcgtcga tctgcgtgcc gagaatctgc aaaccgccgc aaatacccag cagcttgccg
    27301 ccatagcgca ggtgcttggc gatagccgcc ggccagcctt gctgacgcag gaatgccaga
    27361 tcgctgcgaa cgcttttcga gccgggcagg ataatcaggt cagaggcagg gatggcctgg
    27421 ccggggccga caaaggtcag ctcgacctgc ggatgcaggc gcagcgggtc gaaatcggta
    27481 tggttgctga tccgcggcaa taccggcacc accaccttga tccgctcggc gaccttggcg
    27541 ctctggcgta tatcgatggc gtcctcggct tccagatgga aatccatcag gtagggcaaa
    27601 acgccgagca ccggcttgcc ggtgcgctgc tccagccagt ccagccccgg ttgcagcaag
    27661 gcgatatcgc cgcgaaaacg gttgatcaca aagccctgca cccgcgcctg ctcgctctcg
    27721 gaaagcaacg ccagggtgcc gactagatgg gcaaacacgc cgcccttatc gatatcggca
    27781 atcaggatca ccgggcagtc cacagcctcg gcaaaaccca tattggcgat atcgccggcg
    27841 cgcagattga tctccgccgg cgaacccgcg ccctccacca tcaccaccgg ataagcggcg
    27901 ctcaggcgct ggtgcgattg caacaccgcc tgcatcgcca cctttttgta gtcgtgatag
    27961 gccacagcat ccatacagct aagggcgcgg ccatgaataa tcacctgggc accggtgtcg
    28021 ttattaggct tgagcagcac cgggttcata tcagtgtgcg gcaccagccc ggcggcctgg
    28081 gcctgtaccg cctgagcccg gccgatttcg ccgccgtcgg cggtcactgc actattgagc
    28141 gccatattct gcggtttaaa cggcaccacg ctaacgccct ggcgcttaag ccagcggcat
    28201 agcgcagtta ccaaagtgct tttgccggca tccgaagtgg tgccctgcac catcaatgtg
    28261 ctcatcgggc ttccttggca aatagttcta gagcctgctg caaacgctgc caaccggctt
    28321 catcggccgg cagaccaaaa cgcaggctta gcgggctggc aaaaagccgg gtaaggatgc
    28381 ccttagcggc gagaaactcg tgcagctcaa cagccctagg cgtgcagcag aactgaaaca
    28441 gcgcacagcc accggtgggc gcaaaacctt gtgcgctgag tagcacagcc agacgtaggc
    28501 catcagccaa caagcgctca cgctgcaaac gctggccggc gtgatcctgc agcagtgcgg
    28561 tagccaccac ccggctcggc ccgctgacag tccagggtcc gagcaattcg gccagccgtt
    28621 ccagcacagc cggctgggcc aaaacaaagc ccagacgaat accggccagg ccgaagaact
    28681 tgccaaacga gcgcaacaca atcagccccg gccggtcgct aaaagccgcc aggctgaagt
    28741 ctggcgcgca atcaataaag gcttcatcca ccaccagcca gccgcctcgc tcggccaggc
    28801 gggcatgcca gtccagcagg cgctggggct cgatacgtcg gccggtgggg ttattcgggt
    28861 tgaccagcag cagcacatcc agacgctcca gcgcccgctc gatgccgcct tcgctaagct
    28921 ccagcagacg atgaccctcg cgctgccaag cggcagcatg ctcggcatag gctggcgaga
    28981 cgataccgac ccgagcatcc cgcaccagac gcggcagtgc ctggatcgcg gcttgcgaac
    29041 cggctaccgg cagcagattg ctcaccccgt agtaatcgcg ggcggcgatt tccaggccgt
    29101 catgctgctc gggcagccgt gcccaggcac tggcggggat cactggcagc ggccaaccat
    29161 aaggcgcgat accggtggac agatccagcc aatccgccag ggcaatgcca taccgcagcg
    29221 ccgcagcgcg caagcgcccg ccgtgctcaa gcataagaag gttcacgcat aaaaagcccc
    29281 ccacaacagc aacagcgcca accacagcag cacaccgccg cacaccagat tcaacgcccg
    29341 ttcgatatcc cgcgctctgg gctgcggtcc cgcgcccagc tcgggccgct gatgcagctc
    29401 gccgtggtat tccgccgcgc cgcccagact cacgcccagc gcccctgccc cagacgccat
    29461 taccgggccg gcattggggc tgtcccacag cggtgcctgg gtacgccagc agcgcagcgc
    29521 cagcatggtt ttgccaagca atgcataggt cagggcgact aaccgcgcag gcaggtaatt
    29581 aagtacatcg tcgatcttcg ccgcggccca gccgaagcgt tcaaagcgct cattgcgata
    29641 gccccacatg gcgtccaggg tattgctcag gcgatacagc accacgcccg gcgcaccggc
    29701 aatcacaaac cagaacagtg ccgcgaacac tgcatcgctg ccgttttcca gcaccgactc
    29761 gctgcccgcc cgcgccaccc ctgctgcgtc cagctgcgca gtattacggc tgaccatata
    29821 gccgacccgt tcacgcgcca gacgcagatc acccaggcgc aaggcctgcg ctacgggcat
    29881 ggcatgctcg ccgaggcttt tcagacccaa ggcggcgtat agcgcaagaa tctccaccgc
    29941 ccagcccagc ccgctgatct ggctcagcag ccaggccagc agcgtcagcg gtaacaccgc
    30001 caggcaccag gcgctgacgc catggctgcg caaaccgccg cccgccggat tgaagcgttg
    30061 ctcgatacgc tcggccatac gaccaaaggc taccagcgga tgccagccct tgggctcgcc
    30121 aaaaatcgca tccagggcga ccccagcgca ggtgctaaga accaggctca tggcgtgccc
    30181 cactggtttt caaagagcaa ctcgctaagc ggccgaggct cggaccaacc ttgctgcacc
    30241 aacatcggcg cgtcatagaa ttccgtcacc ggccccagac acagcaccgc cagcggcttg
    30301 ctgccggccg gcatgccgag cacatccgcc agggcctgcg gctcgaacag cgatacccag
    30361 cccatgccca aaccctcggc gcgggcagcc agccagaggt tctgaatcgc gcaggccagg
    30421 gatgccaggt ccatttccgg caaggtgcgc cggccgaaca catgggcctc gcggccctcc
    30481 atcagggcca ccaccagtag ctcggcacag tcgcgaatgc cctcgacttt cagccgcata
    30541 aactcgtcgc tgcgctcgcc caagacctca gcggtgcgca ggcgctcctc ctccaccagg
    30601 ccgtgaatcg cctcacgcaa agccgggcgg gtgatgcgaa taaagcgcca cggctgcatc
    30661 aggccgacgc tgggggcctg gtgcgcggct tcgagcaggc gtgcgagcag ttcgggggct
    30721 acctcaccac cgctgaaatg gcgcatatca cggcgctcgg cgatggcgcg atacaccgcc
    30781 gcgcgctcat cggggctaaa ggccaacccg ctcatgctag cgctccgggg caaaacagcg
    30841 cggcggcggc aaccggatca gacggcaaat aaaagtggat ataggaagcc gtcagccggc
    30901 cctggcgata gaccgcttcg gcggtgcgtt tgtagttggg gcactcgccg cgggtcagcg
    30961 gctgcaggtc gctgctcagc gccgagtagt ggaagctgtg gccgcgcagc agcccttccg
    31021 gcagctccgc ctgttgcaag gccatggcgg tcatgcgtgg ttgcagggtc gcctcgccat
    31081 ttagcaagcc gagcatctgc ccgctggcgc cctgtttgtc ggtcagtttg tcgagcaaat
    31141 aaagcatgcc gccgcactcg gcgtggatcg gcttaccggc tgcatggtgc gcacgaatcg
    31201 cctgagccat gggttggttg gcttcaagcg tgaccaggtg cagctccggg tagccgccgg
    31261 gcaaatacag gctatcgaca tcaggtaact gttcatctgt gagcggcgaa aagaactgca
    31321 attcggcgcc cagctcgtgc aacagatcga ggttggcctg gtagagaaag gcaaaggcgc
    31381 tgtcgcgcgc tacaccaatc cgcacgccct tgagcaattg cgcaggccgg gcctgttcgg
    31441 gggtggcgaa gctgactggc gatggcaggc tggtatcggc gctggcagcc agagcatcgg
    31501 cggcggcatc caggcgcgca tccaggtcgg ccagctcgcc cgcctgcacc agacccagat
    31561 gacggctcgg caagccgact tcggcgctgc gcggcagcgc gccgtaccag cggatcgaag
    31621 ctggcagcgc atcacgcaga atctcgccat gtcgcgtgct gccgacccga ttgccgagca
    31681 ccccggagaa cggcagatca ggctgaaagg tcgccaggcc atgggccatg gcaccgaagg
    31741 tctgcgccat ggccgaaccg tcgatcaccg ccagcaccgg cacaccaaag tgccgcgcta
    31801 ggtcggccgc cgagggcgtg ccatcgaaca gacccatcac cccttcgatc agaatcagat
    31861 cggcctcgcc cgccgcttcc caaagcagct tgcgactctc cgctgcgccg accatccaca
    31921 ggtcgagctg atacaccggc gcgccgctgg cgcgggcgag aatcatcggg tcgagaaaat
    31981 ccgggccgca cttgaaaacc cgcacgcgct tgccttggcg tgtatgtaag cgagccaagg
    32041 cagcggtcac cgtggtcttg ccctggccgg aagcgggagc tgcgatcaat agtgcggggc
    32101 aagagcgggc gctgatcaaa actcaacccc cttctgcgcc ttaaccccgg ccttgaaggc
    32161 gtgcttgacc aggttcatct cggtgacggt atccgccgct tcgatcatcc ccggcagcgc
    32221 gccacgaccg gttaccacca catgctgcag cagcggccgc gcttcgatat cggcgagcac
    32281 cgcatcgagt tccaggtaac cgtatttcag cgcgatattc agctcgtcga gcaccaccag
    32341 gccgatggcc ggatcgttca gcagccgcgc cgccaccgcc caggcttcgc gggccttgtc
    32401 gatatcacgc tggcgatcct gggtttccca ggtaaaaccc tcgcccatca cgtgatagct
    32461 gacttcatcg ggaaaacggc ggaagaaggt ttcctcgccg gtgctggccg cgcccttgat
    32521 aaattgcacc acgccgacct tgaggccatg acccagggcg cgcgcgacca tgccgaaagc
    32581 gctgctgctc ttacccttgc cattgccgct gtgcaccagc agcaagccgt actcgtcctg
    32641 ggcctgggca atcttctcgt cgatcagggc tttcttacgg gccatgcgcg ccttgtgccg
    32701 agcgtcacgt tcaatcgatt cagtcatcgc actgctccac cgccacaagg cgatctggct
    32761 ggaaaaagca cgcggagata ggaaaagcgc gggcctgcgc acagcgcaaa ccacacgcca
    32821 gccatcgccc tccgcgatgc cgtggactgc agcaggccgg tctccgggct tatgagcgaa
    32881 ccttgaggtt ctcgaccgcg ccttcccatg cttgagcaca gtggcaatag cggcgtttaa
    32941 ctcacttacc gttgcggggg cagcgtcgga atgactggcg taaagacgct cagcgcaccg
    33001 acttcccagt ttcacctcgc acacgcacag cgtggggggc acctgctaca aggcgcgaag
    33061 ggtagagagt tcgtgattaa gcgtcaaacg aatgcgccgt ttgcacccgt gaatcagtgg
    33121 cggatcaatg cctgggcgta aagcccgcag gcttgctggc cagccactca acaaaagtac
    33181 gcaggcgctc gtcactgagc aggctgtcgc gagtgttgta agtcagcgcc agctcctttt
    33241 cgctgaacaa ccgatggatc tggttgtggc aggcgcggca gacccagagc gtggcgctga
    33301 tgcgctcgct tttgccgaag cgcttctgca catagacctt gttgtgcagc gccttgggga
    33361 tcaggtgatg gcgcgtcaaa gtggtcgtgc gggcgcagag ttcgcaggca gcgggttgtt
    33421 cgggcagacg aatgggttcg ggcataccgg ttcagcgttt acggcatagg ctcaggccgt
    33481 cgcccaacgg caacagggac aagtcgatgc gtggatcatc ccgcagcgcc agattgagcg
    33541 cctgaatcgc tcgggtgtcg gcgctctcgg ggtttgcctc aagcacgcgt ccgctccaca
    33601 aggtgttgtc gaacaggatc agcccaccct tgtgcaccag gcgcagggca tgctcaagat
    33661 aggcagggta attggccttg tcggcatcga taaacagcag atcgatgctg ccagacagac
    33721 cttcagcctc caatgcagcc agggtctgta gcgccggggc caggcgctgt tcgatacgtg
    33781 cgctcaaacc tgcttcgcgc cagtaacgct cggccgtggc gttgtattcg ccgggaatat
    33841 cgcagcagat cagccggcca tcctcaggca tggcctgggc catgcagatg gcgctgtaac
    33901 cggtaaaggt gccgacctcg acaatccgcc gcgcctcagt cagctgcacc agcaaggcca
    33961 taaactgacc ctgctcgggg gcaatctgcc aacgcgccat actcagctgc gcggtttcct
    34021 cacgcagacg tttgagcagc ggtgtttcac gtagggagac atccagcagg tattgataca
    34081 gggcggaatc gagattgagc gttcggttgg tcatgcctac tccctctgaa aaccaaagct
    34141 gtcaggggtt gctcgccagt tccggggtga acacgcgctt ggcattcaga taggtgcgct
    34201 gccagtaagt gttgctcagg ctgtccaggc gcacggtgcc accgctggaa ggtgcgtgaa
    34261 caaagcgccc atcaccgaca tagatgccgg cgtggctgac ctggctaccg ccattggtgg
    34321 cgaaaaatac caaatcccca ctttgcagac ggtcgcgacc gacagccggg gcgtgcatgc
    34381 tgatcagctc gcgggtcgag cgcggcaggc tgatgcccgc cgcatcgcgg tagacataac
    34441 caatcagacc gctgcagtcg aagccgccat ccggggtatt gccgccgtag cgatagggtg
    34501 tgccgaccaa gccgagtgcg cgaaacagta catcttcagc attgccgtta aagctgccga
    34561 tgggtgcatg cacaataggt ttgggagttg gtgcgggggc attgctggcg caggcgctga
    34621 gcagcgcggc aagcacaatc aagctgagac gggcgatagc ggtcatggac gagcgatcct
    34681 gagccgggat gcgccctact ctgccggctt gcacatcgta gtgcaagctt tagctttgct
    34741 tggcaggttg ttgaaaatta tcgagatagg cctggagtat cagttaatcg cagcgaccga
    34801 agagattcag tcgccactca tagggctcaa gggtgacgta cgagcgactc gcttggagcc
    34861 agtgctaggg cacgtttggc ctcgataaag gtgcgcgacc agtagcgatc atccaggcta
    34921 tcgacccgta caccgccgct gcggctgctg ctggagtgga taaactggtt atcacccata
    34981 tagataccgg catggctgac gcggttacgg ccattggtgc tgaagaacag cagatcaccc
    35041 ggctccagct catcacgcga tacctgcggt gcgtcgatat tgatcatgtc gcgggacgag
    35101 cgcggcagtt tcatgcccag ctcttccttg aacagataac cgataaagcc gctgcaatcg
    35161 aagccggatt tcaccgtgct gccaccgtaa cgataacggg tacccaccag cgacaggcca
    35221 tgagtcagga tgctgtcagc cagttgcggc aatttgtagg atttgtcttc agtgaagtcg
    35281 cccagagccg cttctttggc ttgttcgtca aactcggcaa gttctgtcga ggagaacact
    35341 gcaggctggg ttgccagctg cggctgtggg gcatggttgg cgcaggcggc cagaaccacg
    35401 gagagtgcga taggcacgag gggtgcgagg cgtttgagca tgggcacgac cgtgtcggtg
    35461 aatttcacaa gagcgcgact atgccctcta tggcatgcat ttgcaaattc tatggctcta
    35521 aatgtgactc agtagttccg gcaaaacatc taacgcccgc ccttgcagat gcgcattcac
    35581 acgatcgctc aagtcggtgc gctgtgggtt gatttccacg gtaaagccac ccgcctggcg
    35641 ggtacgcagc agcggctcga cgatatacgg aaagctcgcg ctggtgccta tgctgatcac
    35701 cacctcaaaa cctttgcgca cttcggtata gagcgtgtcg atggcctgct cggggagcat
    35761 ttcctcgaac agcaccacgg gcggacgcag taccccggcg cattgccggc atttgggcgg
    35821 cagcggccgt tcaaggtgtt ccgccagctc ggtgtcgaca gcgccacagg actggcaata
    35881 cagcggcgcc agctcgccat gaatctcgat cacccgctcc atcgggctgc cagctgcgcg
    35941 gtggtagccg tcgatattct gggtcagcag ccagcactgc ggctttctac gctgcagctc
    36001 ggcaatcgcg taatgcccag cgttggcctg ggcggccaag caggccttgc ccagctccgc
    36061 caggtatttc cagcacagcg ccggatcacg ctgcagcatc gagccggaca acgcttcctc
    36121 aatcggcaat ccttcggcgg tgtgaccgtt atacaggccg ccgaggccgc gataggtggg
    36181 caaaccggaa tcggcggaca gc
//
LOCUS       DASRUM010000110        11860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_60559, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000110 DASRUM010000000
VERSION     DASRUM010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 11860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11860
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60559"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VFF22_07455"
     CDS             <1..580
                     /locus_tag="VFF22_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transmembrane anchor protein"
                     /protein_id="HZX16685.1"
                     /translation="STLIAAAVAAVLLLTVVMPAEYAIDPTGIGRVLGLTQMGEMKKT
                     QAQDAAPAPVSQASVPIQSAPQEQVTAAPVAEPVAEPQPDLKSDELTVTLKPGEATEI
                     KLEMLDKARVSYEWTTNGVPVNHDTHGEPYNGPKGYYHSYSKAKQIKADKGEFTAIFD
                     GTHGWFWRNRSNSDVTITLKIKGQYLSVKRML"
     gene            complement(662..1144)
                     /locus_tag="VFF22_07460"
     CDS             complement(662..1144)
                     /locus_tag="VFF22_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16686.1"
                     /translation="MSTLDFKRDAGFLAKKQPEPQSQTLPFGFSTASSGLMRMLASAV
                     TLLALTGQPAYASQTVCTFSAGDASRYYELEFIGDDDTNPILVFSSTTFGSGQRITLH
                     SADYSLKQFSPKTRNVSLEFRNPKNFAVPPSFNLNGTGGRAILTTGSNSIEGYLECDY
                     "
     gene            complement(1249..1701)
                     /locus_tag="VFF22_07465"
     CDS             complement(1249..1701)
                     /locus_tag="VFF22_07465"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZX16687.1"
                     /translation="MHLLTEQTTAVACSVQAAYRYASNLERFGEWFPGVIAIESANNL
                     DHAMPGKEYLETVAIPFRGTRKVKLRVKEAEQDKLLVTEGALAPLMPRMEISFQSAGA
                     ESCQITWRMFSRNNGSLARFTLVPLARSVINKRAAIGVAKLKEKLEGL"
     gene            complement(2144..2836)
                     /locus_tag="VFF22_07470"
     CDS             complement(2144..2836)
                     /locus_tag="VFF22_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX16688.1"
                     /translation="MRADQPTVLVIDDEAQIRKFLRISLSAQGYRVLEGGNGRDGLAQ
                     AALDKPDLVVLDLGLPDMDGQAVLRELRAWSQVPVMVLSVRASESEKVLALDGGANDY
                     VTKPFGIQEFLARVRALLRQVTGGSQQDVVVRSAALSVDFAYRRVSLADTEVALTPKE
                     YAVLAMLARHLGRVVTQQHLLKDIWGPSHTDDSHYLRVVVGHLRQKLGDDPTAPQFIV
                     TEAGVGYRLLDV"
     gene            complement(2833..5487)
                     /locus_tag="VFF22_07475"
     CDS             complement(2833..5487)
                     /locus_tag="VFF22_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HZX16689.1"
                     /translation="MSDARRVAAPLDDLPRNGRGRLKVFLGAAAGVGKTYAMLQAAQA
                     QLRQGVDLRVGVVATHSRGETEALLAGLLQQPLLRAQQLGVAVDEMDLDGILANPPRL
                     VLVDELAHSNASGSRHSKRWQDVQELLDAGIDVYSTVNVQHLESLNDRVRDITGVQVQ
                     ETVPDWVLREADEILLIDLPPRELLERLREGKVQMPEQACAAIDAFFSQTNLTALREL
                     AMQTAAARVDADLHQRYRLHGQAAPAVRGRLLVGIDGDGQAERLVRHACQVAERRHLP
                     WSVVHVDTGGKRDETRLGYLQAAQQLAERLGGEVVGLRGEAVARTLIEHAGERRASLI
                     LVGRSRQRLRRRWLGRGLAERLLAQGQGLEISVLDTEVDRAPARRGSRQPLVWYDYLL
                     APLATLIAASVALGLSRFLELPNISLIFLAAVLVVAVCSSLGPALLCAGLSFLAYNFL
                     FIQPTLTLTVARQEDVLNLLFFLLVAGLTGNLASRQRRQLQALRQTQAETTALLDLSR
                     KLTAATDRQAVLAAAMQQFALWRDVDISLLARSRDDIWKVEAGLQRPLAEQERAAADW
                     SWQHEQPAGLGTGTLPAGRWWWWPLVGEDGPLALLGISPREGGALAPEQRRLIAALGQ
                     PLAQALERAQLAANLEAARLHGQTEELRSALLASVSHDLRTPLTAMRGSIDSLLALGE
                     QIPLQDRRELLEGTRDEAERLDRYIQNLLDMTRLGHGSLKLERDWVAPADIVAGAVQR
                     LRPVLAPLQVEIRVPAELPLLYAHAALIEQALVNVLDNAARFSPQQGRLRIGVEADEA
                     ELRFVVSDQGPGIPTHERKKIFDMFYTAARGDRGGQGTGLGLAICQGMVGAHGGQVSV
                     GAGLDGQGASLTLHLPLHPQPLMEEESA"
     gene            5866..7194
                     /locus_tag="VFF22_07480"
     CDS             5866..7194
                     /locus_tag="VFF22_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HZX16690.1"
                     /translation="MKLLLHPARVVALVFLLAILLGSALLMLPIAHAEGMAAPWVTAF
                     FTAVSAVCVTGLVVVDTGTYWSTFGQSVILLLFQLGGFGMMTVATLLGLMVNRSFRLR
                     TKMVAQFESRSLGLGDIASVARLVLVVTFVLELILALLLTLRLRLAYDLPWADAAWNG
                     LFHAVSAFNNAGFSIHADSLMRYATDAWILLPVMTAIVIGGIGFPVLHDLRSRFRDPR
                     HCSLHTKLTLAGTAILLLGGFFALLLFEWSNLATLGPMPVADKVLSAAFASVSARTAG
                     FNSIDIGALTHESWALHYFLMFVGGGSAGTAGGVKVGTAAILILLIIAEIRGHSDSEA
                     FGRRVSASAQRQAITVLVLGSAMIVLGTLTILRQTDFPTDQVIFEVISAFGTVGLSTG
                     ITADLPESSQLMLTLLMYVGRIGTITFAASLALGEHRMPYRYPEEHPIVG"
     gene            7184..7879
                     /locus_tag="VFF22_07485"
     CDS             7184..7879
                     /locus_tag="VFF22_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HZX16691.1"
                     /translation="MLAKFLFTEQFAFSKGDSVVVIGLGRFGGSVAQSLMRLGHDVMG
                     IDQDAEPVHEWADLLTHAVQADSTNLMVLRQLGVADFAHAIVGIGTDLAASLMTIMAL
                     TELGIKDIWVKALTAEHGKIAQRIGAHHVVYPEADMGERVAHLITGRMIDFIEFDDGF
                     AIAKIHAPLPSHNSTLVDSNIRDKYGVTVVGLKRANEDFQHATPSTLILPGDLLIVSG
                     STHLIQKFAGHSR"
     gene            8089..8358
                     /locus_tag="VFF22_07490"
     CDS             8089..8358
                     /locus_tag="VFF22_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HZX16692.1"
                     /translation="MRIVVTALIMTLLAGCATANETFGDGQLCGLHPYCGASTDIMVI
                     KATTAKNAGAMRALAPFAIIDLPFSIVADTLILPYTIFHIKRSEE"
     gene            8686..9339
                     /locus_tag="VFF22_07495"
     CDS             8686..9339
                     /locus_tag="VFF22_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16693.1"
                     /translation="MLKHLWKIVFAATMITSVPALSEESAEPTKDTLAAHNAKNAILK
                     KYSEKQRFSVMNYCMKFEQIPSQMTGALLKEFESKGISPEAYLMAPLCQPEGYSDAIS
                     SPMIHAVADDPNKREETLNNIWLYYSKKRKQPEIFTELLNAKNTQGETFLDYFVTRID
                     RDHYNVVNQYFSIAYIVKFSCEHGATYSTTIKEFDIGVPSDLSYPPNCIAAFRSRLK"
     gene            complement(9610..>11860)
                     /locus_tag="VFF22_07500"
     CDS             complement(9610..>11860)
                     /locus_tag="VFF22_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003182656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HZX16694.1"
                     /translation="RLYRSGDIGRLNAAGDLEFLGRTDHQVKIRGFRIELGEIEARLL
                     QHPAIREAAVIARPGPTDEVELLAYLVCRDACPGAQALRQHLAEQLPAHMLPADYLML
                     AALPLTPNGKLDRAALPAPERGSQRGYRAPRSEREHLLAGLWAEVLGVERVGLDDHFF
                     ELGGHSLTATRLISRLRSALGLEVPVRALFQHPTLASFAGQALPAAPTSSLPPLQPAP
                     AETDLPLSFAQERLWLFDRLHPGNPAYQVAEAVRLTGALDHRALERSFAELLRRHASL
                     RTTFVLVDGQPQQRIAAPRPFPLPLLDLEDLSQAERDQELRLQLDSQALQPFDLARGP
                     LIRAGLIRLEARSHVLWLCLHHIVTDGWSMGVLTRELTALYTAFSAGRPSPLGEPGLH
                     YADFAHWQRRQLGPETLKPQLDFWLNHLGKDRPLLQLPGMRPRPALPSHRGAQHEFGL
                     DIALTRALHDFSQRQGVTLFTSLLAAFKLLLAGHSGQTDIRVGIPIANRNQQALEGLI
                     GFFVNTLALRSDLSDNPSFEALLAQLHEHLLAAHDHQDLPFEQLLQALPTPQQQSAPL
                     LQVMFDLHRERILGSNTFGDLHISPEEEGSNHGTLFDLMLDIGEREQGLVATFTYSTD
                     LFDAAHIATLAADYRHLLRSILERPQVSLDELSAQLSRTPQIAARSALKAETQVYALL
                     EELLEQPVLDPQAGFFEQGGSSLRAVMLCAQLQALWGTAIPPQLVFLHPQLADLVRVL
                     KPYAGRRAY"
BASE COUNT         2301 a         3630 c         3637 g         2292 t
ORIGIN      
        1 ctccaccctg atcgcagcgg ctgttgcagc cgtcttgctg ctcaccgtgg ttatgcctgc
       61 ggaatatgcc atcgatccaa cgggcattgg ccgagtactc ggtctgaccc agatgggcga
      121 aatgaagaaa acccaggcgc aggatgcggc gccagcccca gtctctcaag cgtccgtacc
      181 tattcagtct gcacctcagg aacaagtcac ggccgcacct gttgctgaac cggtagccga
      241 acctcagccc gacctgaaat cggatgaact gaccgtcacg ctgaagccgg gcgaagccac
      301 tgaaatcaaa ctggaaatgc tggacaaggc cagggtcagc tacgagtgga ccaccaatgg
      361 tgttccggtc aatcacgaca cccacggcga gccatacaat ggcccgaagg gctactacca
      421 cagctacagc aaggccaagc agatcaaggc tgataaaggc gaattcaccg ccatctttga
      481 tggtacccac ggctggttct ggcgcaatcg aagcaacagc gatgtgacga tcacgctcaa
      541 gatcaagggc cagtacctaa gtgttaaacg aatgctttaa gacccaaagc gcctccgtca
      601 ttaggcgcag gcgcttgagc aggacggatt gaatgaattg ttaggcccaa ccagaggtga
      661 ctcaatagtc gcactctaga tatccctcaa tgctattgga accggtcgtg agaatggcgc
      721 ggccaccagt cccattgaga ttgaatgatg gcggcacggc gaaattcttt ggattacgga
      781 attccaagct aacgttccga gtctttgggc tgaattgctt aagcgagtaa tcagcggaat
      841 ggagcgtaat gcgttgaccg gaaccgaatg tcgttgagct aaatactaat atcgggttcg
      901 tgtcgtcgtc gccgataaac tccagctcgt agtagcggga tgcatcgccg gccgaaaacg
      961 tgcagacggt ttgtgaggcg taagcaggct ggcccgtgag ggccagtaga gtcacggcgg
     1021 aagcaagcat gcgcatgagg cctgacgagg cggtagaaaa accaaatggc aaagtttgcg
     1081 attgaggctc tggctgtttt ttagccaaaa agccagcatc tctcttgaaa tctaacgtac
     1141 tcatcgcgct tagtttctcc ggatttcgcg cacggcgagc ttcttgagta agccttgagg
     1201 tttttctaca gcctcaacac cggcgcacag cggcgcgctg gttagagttc acagtccctc
     1261 cagcttttct tttagtttgg ctacaccgat tgcggctcgt ttattaatga cgctccgagc
     1321 cagaggcacg agtgtgaaac gcgccagcga gccgttgtta cgactgaaca tcctccaggt
     1381 gatctgacat gactccgcgc cagcgctctg aaagctgatc tccatccgag gcatcaatgg
     1441 cgccagcgcg ccttccgtca ccagaagctt gtcctgttcg gcttctttaa ccctgagctt
     1501 cacctttctt gttccccgga aaggaatggc tacagtctcg agatactctt tgcccggcat
     1561 agcgtgatca agattattcg ccgattcgat ggcgattacc ccaggaaacc attcgccaaa
     1621 gcgctccaga tttgacgcgt accggtaggc agcctggacg gagcaggcta cagcggtcgt
     1681 ctgttcggtc agcaagtgca tacaaacccc caaattaaga tctaatcaag atagggcgac
     1741 gcctcacggc gcctctccgt caacaccacc gtgcatacgg gtcacgtgca cggcggttcg
     1801 atgaatacat tcctaccgcg ccgcaatggc cgcaccccag gttcttgaag taccgcaccg
     1861 gcaatgccaa cgccagtgcc ggagtcttgc tcaaccgcca ggagccatgt accgactcgt
     1921 tggtgtgcca tgctgcttgc acgttgacac cgcgcttgcg caactcccag tcgccggctc
     1981 gagtagcagc gtagtttgcc tcgtgtccat ttgtcgatgc cgcaggacaa ccgggacaac
     2041 cggggacaga ccacgatatt gatagtgcaa ggtgtcagct tttggttcga aagttgccgg
     2101 tcacgaagcc ccggcttggc tctatagcag atcagcaggc tgctcataca tccagcaggc
     2161 gatagccgac acctgcctcg gtgacgataa attgcggagc agtcgggtca tcgccaagct
     2221 tctgccgcag atggccgacc actacacgca ggtagtgact gtcatcggta tggctcgggc
     2281 cccagatatc cttgagcaga tgctgctggg tcaccacccg gccgagatgg cgggcgagca
     2341 tcgccagcac ggcatattcc ttgggtgtca gcgccacctc ggtgtcggcc aggctcacac
     2401 gccgataggc gaagtcgaca gagagcgctg cgctgcgaac caccacgtcc tgctgactgc
     2461 cgccagtgac ctggcgcagc agtgcgcgaa cccgggcgag aaactcctga ataccgaagg
     2521 gcttggtgac gtagtcgttg gccccgccat ccagcgccag caccttctcg ctctcgctgg
     2581 cgcgtaccga cagcaccatg accggtacct gcgaccaggc ccgcaactcg cgcagcacgg
     2641 cctggccgtc catgtccggt aaacccagat cgagcaccac cagatcaggc ttgtccagcg
     2701 ccgcctgcgc taggccatca cggccattgc cgccttcgag tacccggtag ccctgggcgc
     2761 tgaggctgat acgcaagaac ttgcggatct gcgcttcgtc atcgataacc agaacggtcg
     2821 gctggtcggc tctcatgcag attcttcttc catcagaggt tgtgggtgca gcggtagatg
     2881 cagggtcagg ctggcacctt ggccgtccag gcccgcgccg acgctgacct gcccgccgtg
     2941 cgcgcccacc atgccctggc agatggccag gcccaggccg gtgccttggc cgcctcgatc
     3001 gccgcgcgcg gcggtataga acatatcgaa aattttcttg cgttcgtgcg tgggaatccc
     3061 cggtccctga tcgctgacga caaaccgcag ctcggcctcg tccgcctcca caccgatgcg
     3121 taaacgcccc tgctggggtg aaaaacgcgc ggcattgtcc agcacgttga ccagggcctg
     3181 ctcgatcagt gcggcatggg catagagcaa cggcagctcg gccggtaccc ggatctcaac
     3241 ttgcaaaggt gccagcaccg gacgcaggcg ttgcaccgca ccggccacga tatcggccgg
     3301 cgccacccag tcgcgctcca gcttgaggct gccatggccc aggcgggtca tatccagcag
     3361 attctggata tagcggtcca ggcgctcggc ctcatcgcgc gtgccttcca gcagctcgcg
     3421 acggtcttgc agcgggatct gttcaccgag tgccagcagg ctgtcgatgg agccgcgcat
     3481 ggcggtcaag ggagtacgca aatcatggga caccgaggcc agcagggcgc tacgcagctc
     3541 ctcggtctgg ccatgcaggc gcgcggcctc caggtttgcg gccagttgcg cacgctccag
     3601 ggcctgggcc aatggctggc caagggcggc gatcagtcgg cgctgctcgg gtgccagcgc
     3661 tccgccttcc ctggggctga tgcccagcag tgccaacggg ccatcttcgc caaccagtgg
     3721 ccaccaccac cagcgcccgg cgggcaaggt tccggtaccc aggccggccg gttgctcatg
     3781 ctgccaggac cagtcggcgg cagcgcgttc ctgttcggca agaggacgct gtagaccggc
     3841 ttcgactttc catatgtcgt cacggctgcg ggccagcagg ctgatatcca cgtcacgcca
     3901 gagtgcgaac tgttgcatcg ccgccgccag cacagcctgc cggtcggtgg cggcggtcag
     3961 cttgcgcgac agatccagca atgccgtggt ttccgcctgg gtctggcgca aggcctgcag
     4021 ctgtcggcgc tggcggctgg ccaggttacc cgtgaggccg gccacgagaa gaaagaacag
     4081 caggttcagc acatcttcct ggcgcgcgac ggtcagggtc agggtcggct gaataaacag
     4141 aaagttgtag gccagaaagg acaagccggc gcagagcagg gccgggccca gactgctgca
     4201 caccgccacc accagcacgg cggcgagaaa aatcagggaa atattcggca attcgagaaa
     4261 gcgcgacagc cccagcgcaa cactcgccgc gatcagggtg gccagcggcg ccagcaggta
     4321 gtcataccag accagcggtt ggcggctgcc gcgccgcgcg ggagcgcgat caacctcggt
     4381 gtcgaggaca ctgatttcca ggccctggcc ctgcgcgagt aaccgctcgg ccaggccgcg
     4441 accgagccag cgacggcgca accgctgacg gctgcggcct accaggatca ggctggcgcg
     4501 gcgctcgccg gcatgctcga tcagggtgcg tgccaccgcc tcgccgcgca ggccgaccac
     4561 ttcgccgccg aggcgctcgg ccagctgttg ggcggcctgc aggtagccga gacgcgtttc
     4621 gtcgcgtttg ccgcccgtat ccacatgcac cacggaccag ggtaaatgac gccgctctgc
     4681 cacctggcag gcatgccgga ccaggcgttc agcctgccca tcgccgtcta tcccgaccag
     4741 taaacgacca cgcaccgccg gggcggcctg cccatgcagg cgataacgct gatgcaggtc
     4801 ggcatcgact cgggcggcgg cagtctgcat cgccagttcg cgcagggcgg tcaggttggt
     4861 ctggctgaag aaggcatcaa tggccgcgca cgcctgctcc ggcatctgga ccttgccttc
     4921 gcgcaagcgc tcgagcaatt cccgcggggg cagatcgatc aggaggattt catcggcttc
     4981 ccgcagaacc cagtccggta cggtttcctg cacctgcacc ccggtaatgt cgcgcacccg
     5041 atcgttaagg ctctccaagt gctgcacgtt gaccgtgctg tatacatcga tgccggcatc
     5101 gagcagctcc tgcacatcct gccagcgctt gctgtggcgg ctgccggagg cgttgctgtg
     5161 agccagctcg tcgaccagta ccagcctggg cggattggcc aggatgccgt cgaggtccat
     5221 ctcgtcgacg gccacgccca gctgctgtgc gcgcagcagg ggttgctgca gcaagccggc
     5281 gagcagggcc tcggtttccc cacgactgtg ggttgccacc accccgacgc gcaggtcgac
     5341 gccctgacgc agctgcgcct gcgctgcctg cagcatggcg taggtcttac cgaccccggc
     5401 ggcagcaccg agaaagacct tcagccggcc gcgcccattc ctgggcaggt catccagcgg
     5461 ggcggcgacg cgtcgagcat cactcatggg cctgactcgg tatttatgga gggcgaattg
     5521 tcggcccgtg tttggttgag cgccagcaca ttgcagcgcg gaggaacgct ttgggcgatt
     5581 ggcgtaaagg ttcgctacgc gaaggctgaa ggcgtgtaaa aaaatagtaa attttcgtcg
     5641 cgcagcccgc agggcaacag cgggcgagat tgaatcagag gtagcatccg gcatctgaac
     5701 cggttcccag cgaaaagaac ccctgactcc cagcgagtga cccgcaggcc tggccatcgg
     5761 ctggaatgag gctgcatcag cgcacccgcg cgcccacctt gagaagcgtc ccccaatgca
     5821 cgcaagcctt cgcccatgcc tcccgaccct gcgggcgacc gtcaaatgaa attactgctt
     5881 catcccgcca gggttgtcgc tctggttttt ctgctcgcga ttctgctcgg tagcgcgctg
     5941 ctgatgctgc cgatagccca cgccgagggt atggctgcac cctgggtgac cgcatttttc
     6001 accgcggtat cggccgtgtg cgtgaccggg ctggtcgtgg tggacaccgg aacctactgg
     6061 tcgacctttg gccaatcggt gattctgctg ctgtttcagc ttggcggctt cggcatgatg
     6121 acggtggcga ccctgctcgg cttgatggtc aatcgctcct ttcgcctacg caccaaaatg
     6181 gtcgcccaat tcgagtcgcg ttcattgggg ctgggcgaca ttgccagcgt ggccaggctc
     6241 gtgctggtgg tgacgttcgt cctggaactc attctggctc tattactcac ccttagatta
     6301 cgcctggcct atgacctgcc ctgggccgac gcggcgtgga acgggctgtt tcatgccgta
     6361 tccgcattca ataacgcggg cttctcgatt cacgccgaca gcctgatgcg ctatgccacc
     6421 gatgcatgga tcctgctacc ggtgatgacg gcgattgtca ttggtggcat cggtttcccc
     6481 gtcctgcatg acttgcgtag ccgattcagg gatccccggc actgctcact gcataccaaa
     6541 ctcaccctag ctggcacggc catattgctg ctgggcggat ttttcgcctt gctgctgttc
     6601 gagtggtcca acttggccac cctcggcccc atgcctgtgg ccgataaagt gctttcggcg
     6661 gcatttgcgt cagtttctgc gcgtactgcc ggcttcaact cgatcgacat cggcgccttg
     6721 acccatgaaa gctgggcatt gcactacttc ctgatgtttg tcggcggcgg cagcgcgggt
     6781 accgccggtg gtgtcaaggt cggcacggct gccatcctga tcttgctgat catcgccgag
     6841 atacgtggcc acagtgacag cgaggcattt ggccgcagag tcagtgcctc ggcccagcgc
     6901 caagccatca ccgtgctcgt gctgggcagc gccatgatcg tgttggggac gctgaccatc
     6961 ttgcgccaaa ccgatttccc caccgatcag gtcattttcg aagtcatctc ggcgtttggc
     7021 acggtcggac tgtccaccgg catcactgcc gatctccccg agtcgagcca gctgatgctg
     7081 acgctgctga tgtatgtcgg gcggatcggc actatcacgt ttgcggcatc actggccctg
     7141 ggcgagcacc gcatgcccta ccgctaccca gaggagcatc ccattgttgg ctaagtttct
     7201 gtttaccgag cagtttgcct tttccaaggg cgatagcgtg gtggtgattg gcctcggccg
     7261 cttcggcggt tccgtggctc aatcactgat gcgcctgggc catgacgtca tgggcatcga
     7321 ccaggacgcc gaacccgtgc atgaatgggc cgatctgttg acgcatgcag tccaagccga
     7381 ctccaccaac ctcatggtct tgcgtcaact gggtgttgcc gactttgctc acgccatcgt
     7441 ggggatcggc actgacctgg cagccagcct gatgaccatc atggcgttga ccgaactggg
     7501 gatcaaagat atctgggtca aggccctgac tgccgaacat ggaaaaatag ctcaacgtat
     7561 tggcgcgcac catgtggtct acccggaggc cgatatgggg gagcgtgtgg cgcacctgat
     7621 taccgggcgg atgatcgact tcatcgagtt cgatgacggc tttgccatcg ccaagattca
     7681 cgcgccgctg ccgagccaca acagcacctt ggttgactcc aatattcggg acaaatatgg
     7741 cgtcactgtg gtcgggctga agcgggccaa tgaagacttc cagcatgcca cgcctagcac
     7801 tctgatcctg ccgggcgatc tccttatcgt gtccggctca acccatctga tccagaaatt
     7861 tgcgggccac tcgagataac cagagagggg gcactgttta gtgaccccgt gagcgatgag
     7921 gaagaggtcc agaacttcct tactgctact gatgctgatg ccatcaatac acaactacgc
     7981 tccccatttc ctggtaataa cgcttgtgca gcgacagatc gaggtcagta cctgtaggcc
     8041 gctcgctatc aatgctacag tcccgtactt tcaggatgag ggaacgtcat gcggatcgta
     8101 gtaacggctt taataatgac actgctggca ggatgcgcga ctgccaacga aacctttggc
     8161 gatggacaac tctgcggtct ccatccttac tgcggcgctt ctaccgatat catggtgatc
     8221 aaggccacga cggcaaagaa cgctggagcc atgcgcgcgt tggcaccgtt tgcaattatc
     8281 gacctccctt ttagtattgt cgccgatacg ttaatcctgc cctataccat ttttcatata
     8341 aagcgttccg aggagtagtt gtatcccggc gcccccaaaa ccactcagta tctatcaagg
     8401 gtcacgtctg gctgacggcc agggtaaaac ggcgcgcctg gttgattgca gtcccccgca
     8461 ctacaccgcg ctgtgagtgc aagatccggt gtgtgaggag cgttagtagc ggttcaacgc
     8521 aatggccgct ttcgaccaga aaccgccgtt tagaagcatc aataccctgt ttgcgcccga
     8581 ttgtttcctg attattgcct aactcaggcg atactggtgc ccggtgcagt cgagtgtgta
     8641 aactggtccc gtcaatacgg tcgctagccc taatcggagt aatcaatgct taagcacctc
     8701 tggaaaatcg ttttcgcagc cacgatgatc acctccgtgc ctgcactttc agaggagtca
     8761 gcagagccca caaaagacac tttggcagct cataatgcaa aaaatgccat cctcaaaaaa
     8821 tacagtgaga aacagcggtt tagcgtgatg aattactgca tgaagtttga gcaaattcct
     8881 tcgcagatga ccggagcgct tctcaaggag tttgagtcaa aaggtatatc ccctgaagct
     8941 tatctgatgg ctccgctgtg ccagccagag ggttactcag atgctatatc aagcccgatg
     9001 attcacgccg ttgccgatga tccaaacaag cgcgaggaga cgctgaacaa tatctggctt
     9061 tactactcga aaaaacgcaa gcagccagaa atattcactg aattactgaa tgccaaaaat
     9121 acacagggag aaacgtttct cgattacttt gtcaccagaa ttgaccgaga tcactacaat
     9181 gtggtgaacc agtacttttc aatagcatat atcgttaagt tttcttgcga gcatggcgct
     9241 acttactcaa ccacaattaa ggagtttgat ataggtgtgc cgtctgatct tagctaccca
     9301 ccaaattgta ttgcagcatt cagaagtaga ttgaaatagg tttcagagta agaatctcag
     9361 ttgaataggc gaatatcacg aaggctccag gcatcaggca tcaggcatca ggcatccaac
     9421 tgactgccag tgattcacga acaatcgggg gcagaccacg atattgatgt ttctgttcgg
     9481 cggcatctag ccaactcccg cctgccataa ccgcccgctc ggggcgtttc acctgattct
     9541 ccaggagtcg atggtccgtc ggctatgggg cgagcggcct gctcgggtcg ctcgcgcatt
     9601 tgtcacgtct caataggccc gcctgcccgc atagggtttc agcacccgca ccagatcggc
     9661 cagctgtggg tgcaggaaca ccaactgcgg cggaatcgcc gtgccccaga gtgcttgcag
     9721 ttgggcgcag agcattacgg cccggagcga gctgccgcct tgttcgaaga agcccgcctg
     9781 gggatccagt acgggttgct ccagcagttc ctccagcagg gcgtagacct gggtttccgc
     9841 tttcagcgcg ctgcgggcag cgatctgggg cgtccggctc agttgggcgc tcagttcgtc
     9901 caggctgacc tgcggccgct ccaggatgct ccgcaacagg tgccggtagt ccgcggcgag
     9961 ggtggcgata tgggccgcgt cgaacaggtc ggtgctgtag gtgaaggtgg cgaccaggcc
    10021 ctgttcgcgc tcgccgatat ccagcatcag gtcgaacagg gtgccgtggt tgctgccttc
    10081 ctcctcgggg ctgatgtgca ggtcgccgaa cgtgttggag ccgaggatgc gctcgcggtg
    10141 caggtcgaac atcacctgga gcagcggcgc gctctgctgc tggggcgtgg gcaacgcttg
    10201 cagcagttgc tcgaacggca ggtcctggtg gtcgtgggcg gccagcaggt gctcgtgcag
    10261 ctgggccagt agggcctcga acgaggggtt gtcgctcagg tcgctgcgca gggccagggt
    10321 gttgacgaag aagccgatca gcccctccag cgcttgctgg ttgcggttgg cgatggggat
    10381 gccgacccgg atatcggtct gcccgctgtg gccggccagc agcaatttga aggccgcgag
    10441 caggctggtg aacagggtca cgccctggcg ttggctgaag tcgtgcagcg cccgggtcag
    10501 ggcgatgtcc agaccgaatt catgctgggc gccgcgatgg ctgggcagcg ccggccgcgg
    10561 gcgcatgccg ggcaactgca gcagcggcct gtctttgccc agatgattga gccagaagtc
    10621 cagctgtggc ttgagggtct cggggccgag ttggcggcgc tgccaatggg cgaagtcggc
    10681 gtagtgcagc ccaggctcgc ccaggggcga aggcctgccg gcgctgaacg ccgtgtagag
    10741 cgccgtcagt tcgcgggtca gcacgcccat ggaccagccg tcggtgacta tgtggtgcag
    10801 gcacagccag agcacatggc tccgcgcttc caggcgtatc agcccggcgc ggatcagcgg
    10861 tccgcgggcc aggtcgaagg gctgcagcgc ctggctgtcc agctgcaggc gcagttcctg
    10921 gtcgcgctcg gcctggctca ggtcttcgag gtccagcagc ggcaagggga agggccgtgg
    10981 cgcggcgatg cgctgttgcg gctggccgtc caccagcacg aaggtggtgc gcaggctggc
    11041 atgccggcgc agcaactcgg cgaagctgcg ttccagcgcc cggtgatcca gtgcgccggt
    11101 cagtcgcacc gcctcggcga cctggtaggc cgggttaccc gggtgcaggc gatcgaacag
    11161 ccacaggcgc tcctgggcga aggacagcgg caggtccgtc tcagccggtg ccggctgcag
    11221 cggcggcagg ctggaggtgg gggcggccgg caatgcctgc ccggcgaagc tggccagggt
    11281 cgggtgctgg aacagcgcgc gcaccggcac ttccaggccc agggcgctgc gcaggcgcga
    11341 gatcaagcgg gtggcggtca gcgagtggcc gcccagttcg aagaaatggt catccaggcc
    11401 gactcgctcg acccccagca cctcggccca gaggccggcc agcaggtgct cccgctcgct
    11461 gcgcggcgcc cgatagccgc gctggctgcc gcgctcggga gcgggcaggg cggcgcggtc
    11521 gagcttgccg ttgggcgtca ggggcaaggc cgccagcatc aggtaatcgg ccggcagcat
    11581 atgagccggc aactgctcgg ccaggtgctg gcgcagcgcc tgggcaccgg ggcaggcatc
    11641 gcggcagacc aggtaggcca gcagctcgac ctcgtcggtc gggcccgggc gggcgatcac
    11701 cgcggcttcg cgaatggctg gatgctgcag caggcgcgcc tcgatctcgc ccagctcgat
    11761 gcgaaaaccg cggatcttca cctggtggtc ggtgcgaccg agaaactcca ggtcgccggc
    11821 cgcgttcagc cggccgatat cgccgctgcg gtagaggcgg
//
LOCUS       DASRUM010000111         4715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1403550, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000111 DASRUM010000000
VERSION     DASRUM010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403550"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1434)
                     /gene="sthA"
                     /locus_tag="VFF22_07505"
     CDS             complement(40..1434)
                     /gene="sthA"
                     /locus_tag="VFF22_07505"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HZX16695.1"
                     /translation="MAVYNYDVVVLGSGPAGEGAAMNAAKAGRKVAVVDSRREVGGNC
                     THLGTIPSKALRHSVRQIMQFNTNPMFRQIGEPRWFSFPDVLKSAERVIAKQVTSRTG
                     YYARNRIDVFFGTGSFADEQTVEVVCPNGVVETLVAKQIVIATGSRPYRPADVDFHHP
                     RIYDSDTILSLGHTPRRIIIYGAGVIGSEYASIFSGLGVLVDLIDNRDQLLSFLDAEI
                     SDALSYHLRNNNVLIRHNEDYESVEGVDNGVVLHLKSGKKIKADAFLWCNGRTGNTDQ
                     LGLENIGIKVNGRGQIEVDQSYRTQVSNIYAAGDVIGWPSLASAAADQGRSAAGSIVD
                     NGSWRFVDDVPTGIYTIPEISSIGKNERELTQAKIPYEVGKAFFKGMARAQISHEPVG
                     MLKLLFHRETLEVLGVHCFGYQASEIVHIGQAIMNQKGEANTIKYFVNTTFNYPTMAE
                     AYRVAAFDGLNRLF"
     gene            complement(1558..1785)
                     /gene="nqrM"
                     /locus_tag="VFF22_07510"
     CDS             complement(1558..1785)
                     /gene="nqrM"
                     /locus_tag="VFF22_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HZX16696.1"
                     /translation="MTFLVVFLVMVLVVGMMAVGVMMGRKPIAGSCGGIANLGIEKEC
                     SICGGSREKCEEINSDSQGKTEDLAYDATKR"
     gene            complement(1782..2705)
                     /locus_tag="VFF22_07515"
     CDS             complement(1782..2705)
                     /locus_tag="VFF22_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZX16697.1"
                     /translation="MGSTYTVKYVRSKDSPSVELLKRETDAILAEVDVQMSTYRDDSA
                     IEQFNHAPAGTCQAMPDGVLELVDAGRVLHEQSQGAFDLTLEPLLNLWGFGPKARAEK
                     VPTAEQLTAARARVGMQHLRRDGQQLCKEVDVQVDFNSIAAGYAVDKLVQHFTELGVT
                     SYMVEVTGELKAAGKKPDGQPWRIGLEAPQDGQRVAQRVLAVDGYGISTSGDYRNYFE
                     EEGQRYSHTLDPLTGAPITHKLAAVTVVDKSTLRADGLSTVLMVMGTERGLAFAERMG
                     IAAFFVTREGDAFVTQTTQAFEQLFAAGDEQ"
     gene            complement(2810..4036)
                     /gene="nqrF"
                     /locus_tag="VFF22_07520"
     CDS             complement(2810..4036)
                     /gene="nqrF"
                     /locus_tag="VFF22_07520"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HZX16698.1"
                     /translation="MINLEILIAIGMFTGIVLALVLVILAARAKLVSSGDVSIEINGE
                     KTIVVPAGGKLLQTLAANNIFLSSACGGGGTCAQCKCVVEHGGGEMLPTEEAAFTRRE
                     AKAGWRLSCQTPVKQDMKIEVPEEVFGVKKWQCTVESNPNVATFIKELTLKLPEGESV
                     DFRAGGYVQLECPPHEVHYKDFDIQEEYRGDWDKFNQWKYVSKVEETVIRAYSMANYP
                     EEVGLVKFNIRIASPPPGKDDLPPGKMSSYVFSLKPGDKIDVYGPFGEFFAKDTDAEM
                     VFIGGGAGMAPMRSHIFDQLKRLKSTRKISFWYGARSMREAFYVEEYDQLQAENPNFK
                     WHLALSDPLPEDNWTGLKGFIHNVLYENYLKDHPAPEECEFYMCGPPMMNAAVIKMLI
                     DLGVEEENILLDDFGG"
     gene            complement(4052..4660)
                     /gene="nqrE"
                     /locus_tag="VFF22_07525"
     CDS             complement(4052..4660)
                     /gene="nqrE"
                     /locus_tag="VFF22_07525"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HZX16699.1"
                     /translation="MEHYLSLLAKAVFVENMALAFFLGMCTFIAISKKVETAIGLGIA
                     VIVVQVITVPANNLIYTYLLKDGALAWAGLPEVDLSFLGLLSYIGVIAAIVQILEMLL
                     DKYVPSLYNALGVFLPLITVNCAIMGGTLFMVERDYNLAESTVYGFGSGLSWALAIAA
                     LAGIREKLKYSDVPAGLQGLGITFITIGLMSLGFMSFSGVQL"
BASE COUNT          978 a         1465 c         1311 g          961 t
ORIGIN      
        1 ggttctcccc ggccggctca ggccaccggc cggagccgct caaaaaagcc ggttgaggcc
       61 gtcgaacgcc gccacccggt aggcttccgc catggtcggg tagttgaagg tggtgttgac
      121 gaaatacttg atggtattgg cctcaccctt ctggttcatg atcgcctggc cgatatgcac
      181 gatctccgag gcctggtagc caaagcagtg cacgcccagc acttccaggg tttcacggtg
      241 gaacagcagc ttcagcatgc ccactggttc gtgggaaatc tgcgcccgcg ccataccctt
      301 gaagaacgcc ttgccgacct cgtaggggat cttggcctgg gtcagctcgc gctcgttctt
      361 accaatcgag ctgatttcag gaatggtgta gataccggtt ggcacgtcat cgacgaagcg
      421 ccagctgcca ttgtcgacga tgctaccggc agctgaacga ccctggtcag ctgcggcact
      481 ggccaggctc ggccagccga tcacgtcacc agcggcgtag atgttcgaca cctgagtgcg
      541 gtaactctgg tcgacttcga tctgcccacg gccgttgacc ttgatgccga tattttccag
      601 gcccagctga tcggtgttgc cggtacggcc attgcaccag agaaaggcgt cggccttgat
      661 cttcttgccg gacttgaggt gcagaaccac cccgttatcc acgccttcaa cgctttcgta
      721 gtcttcgtta tggcgaatca gcacattgtt attgcgcagg tggtagctca gcgcatcgga
      781 gatttccgca tcgaggaagc tcagcaactg atcacggttg tcgatcagat caaccagcac
      841 gcccaggccg ctgaagattg aggcgtactc agaaccgatc accccggcac cgtagatgat
      901 gatacggcgc ggggtatggc cgagactgag gatagtgtcg ctgtcataga tacgcgggtg
      961 gtgaaagtcc acgtccgccg gacggtaagg ccgcgagccg gtggcgatga cgatctgctt
     1021 ggccaccagt gtttccacca cgccattggg gcacaccact tcgacggtct gctcgtcggc
     1081 aaagctgccg gtgccaaaga acacatcgat acggttacgt gcgtaataac cggtacgcga
     1141 ggtcacctgc ttggcgatca cccgctcggc gcttttcagc acatccggga aggagaacca
     1201 gcgcggctcg ccgatctggc ggaacatcgg gttggtgttg aactgcatga tctgccgcac
     1261 cgaatggcgc agcgccttgg acgggatggt gcccaggtgg gtgcagttgc cgccgacttc
     1321 gcggcggctg tcgaccactg ccaccttgcg cccggccttg gccgcgttca ttgccgcgcc
     1381 ttcacccgca gggccggaac ccagcaccac tacgtcgtag ttgtagaccg ccatgtttac
     1441 tcctcagatc acaccggagc acttgtagcg cactctcggc agggccagca ctgcgcacgc
     1501 agcctgacaa tcaattcgtt cgctgcaagc ctagccttta agccggccgc aggacgatta
     1561 gcgtttggtc gcgtcgtagg ccaggtcttc ggttttgcct tgcgagtcgc tgttgatctc
     1621 ctcgcatttc tcgcggctgc caccacagat cgagcattcc ttctcgatac ccagattggc
     1681 gataccgccg cacgaaccgg cgatgggctt gcgccccatc atcacgccta cagccatcat
     1741 gccgactacc agcaccatga ccagaaacac cactaaaaat gtcattgctc gtctcctgca
     1801 gcgaatagct gctcgaaagc ctgagttgtc tgtgtgacga aagcgtcacc ttcacgggtc
     1861 acgaaaaacg ccgcaatacc catgcgttca gcaaatgcca ggccacgctc agtgcccatc
     1921 accattagca ctgtagacaa gccatcggcg cgcaaggtcg atttgtccac cacggttacc
     1981 gctgccagct tgtgggtgat cggcgcaccg gtcaacggat cgagggtgtg cgagtagcgc
     2041 tggccctctt cctcaaagta attgcggtaa tcacccgaag tggaaatgcc gtagccatcc
     2101 accgccagca cccgctgggc gacacgctgg ccgtcctgcg gggcttccag gccaatgcgc
     2161 caaggctgac catcgggctt tttacccgcg gccttaagct cgccggttac ttcaaccata
     2221 tagctggtca cgcctagctc agtgaagtgc tgcacaagct tgtccaccgc atagccggcg
     2281 gcaatgctgt tgaagtcgac ctgaacatca acctccttgc acagctgctg cccatcacga
     2341 cgcagatgct gcatgcccac ccgcgcacgc gcggcggtca gttgctcagc ggtcggcacc
     2401 ttttcggcgc gcgctttagg gccaaaaccc cagagattga gcagcggttc cagggtcagg
     2461 tcgaatgcac cctgactttg ctcatgcaac acgcgcccgg cgtcaaccag ctccagcacc
     2521 ccgtccggca tggcctgaca ggtacccgct ggggcatggt tgaattgctc gattgcggaa
     2581 tcatcgcgat aggtcgacat ctgcacgtca acctcagcca ggattgcatc ggtctcgcgc
     2641 ttgagtagct caacgctcgg cgaatccttg ctgcgcacgt atttgaccgt gtaggtgctg
     2701 cccatggtcg ggccgccaaa ctcttctaca ggctctgaaa accaacagcc cgttagggct
     2761 gccagcaagc tgacagcgat aacgggccgc agtagcacat ggatcatgct taaccgccga
     2821 agtcgtcgag caggatgttc tcctcctcca cacccaggtc gatcagcatc ttgatcacgg
     2881 ctgcgttcat catgggcggg ccgcacatgt agaactcaca ctcttccgga gcaggatggt
     2941 ccttgaggta gttttcgtac agcacgttgt ggataaagcc cttgagaccg gtccagttgt
     3001 cttccggcaa cggatcggac agcgccagat gccacttgaa gttggggttc tcggcctgca
     3061 gctgatcgta ttcttcaacg tagaaggctt cacgcatgga gcgcgcgccg taccagaagc
     3121 tgatcttgcg ggtcgacttc aaacgcttga gctggtcgaa gatgtgcgaa cgcatcggcg
     3181 ccataccggc accaccaccg ataaacacca tctcggcgtc ggtgtccttg gcgaagaact
     3241 caccaaacgg gccatacaca tcgatcttgt cgcccggctt gaggctgaac acataggaac
     3301 tcatcttgcc cggcggcaga tcgtccttgc ccggtggcgg cgaagcaata cggatattga
     3361 acttcaccaa accgacttct tccgggtagt tggccatgga gtaggcacgg atcacggtct
     3421 cttcgacctt agacacgtac ttccactggt taaacttgtc ccagtcaccg cggtactctt
     3481 cctgaatatc gaagtccttg tagtgcactt cgtgcggcgg gcattccagc tgcacatagc
     3541 caccggcacg gaagtcgacg ctttcgcctt ccggcagttt cagcgtcagt tctttgatga
     3601 aggtggccac gttggggttg gactcaacgg tgcattgcca cttcttcacg ccgaacactt
     3661 cttccggcac ttcgatcttc atgtcctgct tgaccggagt ctggcaggac agacgccaac
     3721 cagccttggc ttcgcgacga gtgaaggcag cctcttcggt cggcagcatt tcaccaccgc
     3781 catgctcgac cacacacttg cactgggcgc aggtaccgcc gccgccgcac gccgaagaca
     3841 ggaagatgtt gttggcagcc agggtctgca gcaacttgcc gcccgcgggt accacaatgg
     3901 ttttttcgcc gttgatctcg atgctcacgt caccactgga aaccagcttg gcgcgcgccg
     3961 cgagaatgac caacaccagc gccagcacga tgccggtgaa catgccgatg gcgataagaa
     4021 tctctaaatt gatcatctat gcatcccttc cttacaactg cacgccggag aaggacataa
     4081 agcccagaga catcagaccg atggtgataa aggtgatacc caggccctgc aggccagccg
     4141 gaacgtcgct gtacttgagc ttctcgcgga tgcccgccag tgcggcaatc gccagcgccc
     4201 aggacagacc ggagccgaag ccatacacag tactttccgc caggttgtag tcacgctcga
     4261 ccatgaacag ggtgccgccc atgatggcgc agttcaccgt gatcagcggc aggaacaccc
     4321 ccagggcgtt gtagaggctg ggtacgtact tatccaggag catctcgagg atctgcacga
     4381 tggccgcgat caccccgata tagctgagca gaccgaggaa actcaggtcc acttccggca
     4441 aaccggccca ggccagcgcg ccatctttca gcaggtaggt gtagatcagg ttgttcgcag
     4501 gcacggtaat gacctgcacc acgataaccg cgatacccag gccaatcgcc gtctcgactt
     4561 tcttcgagat ggcgataaag gtgcacatgc cgaggaagaa ggccaatgcc atgttctcaa
     4621 cgaacaccgc cttggccagc aagctgaggt aatgctccat tagtaagcct ccttattcga
     4681 gacctgcgga gccatcttga agcttggctt ctcca
//
LOCUS       DASRUM010000112         9591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1236179, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000112 DASRUM010000000
VERSION     DASRUM010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9591
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236179"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..543)
                     /gene="pal"
                     /locus_tag="VFF22_07530"
     CDS             complement(46..543)
                     /gene="pal"
                     /locus_tag="VFF22_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HZX16700.1"
                     /translation="MEMLKFGKFAALSLALAVAVGCSSKGGDAAGEGATDPNAGYGAN
                     TGAVDGSLSEEAALRAITTFYFEYDSSDLKPEAMRALDVHAKDLQGNGARVVLEGHAD
                     ERGTREYNMALGERRAKAVQRYLVLQGVSPAQLELVSYGEERAIATGNDESSWAQNRR
                     VELRK"
     gene            complement(610..1908)
                     /gene="tolB"
                     /locus_tag="VFF22_07535"
     CDS             complement(610..1908)
                     /gene="tolB"
                     /locus_tag="VFF22_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HZX16701.1"
                     /translation="MNTLMRIVLLGLTLMVGTVQAADPLVISSGTDRATPIAVVPFGW
                     QGGNVLPEDMAEIIGNDLRNSGVFEPIPRQNMISLPTQATEVIYRDWQALGAQYVLVG
                     SIVPNAGRLQIQFALFNVTTQQQVMTGSVGGGVDQLRDMAHHIADQAYEKLTGVKGAF
                     STRMLYVTAERFSVSNTRYTLQRSDYDGARAVTLLQSREPILSPSFAPDGKRIAYVSF
                     EQKRPRIFVQHIDTGRREQITNFEGLNGAPAWSPDGNRLAFVLSRDGNPEIYVMDMGS
                     RQLRRVTTQPSIDTEPFWGKDGQTLYFTSDRSGKPQIYKTNINGGSAERVTFIGNYNA
                     NPKLSADEKTLVMIHRQDGYTVFKVAAQDLQRGNLRILSDTSLDESPTVAPNGTMVIY
                     ATRQQGRGVLVLASTNGRVRLPLPTAQGEVREPSWSPYLN"
     gene            complement(1905..2855)
                     /gene="tolA"
                     /locus_tag="VFF22_07540"
     CDS             complement(1905..2855)
                     /gene="tolA"
                     /locus_tag="VFF22_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HZX16702.1"
                     /translation="MHYQRERSPSESLFWPVVWAVGLHVIMFAMLFVSFAFAPDLPPA
                     KPVVQATLYKLQSQSQATTQTNQKIAGETKKTTAPVYETEQLEQKKVEQEKVAAKAAE
                     QKKAQEAQKAEAAKKADADKKTAEQKKLADVAKKKAADEAAKKKAAEDTKKKAAEEAK
                     KKAAAEAAKKKKADDAKKKAAEAAQRKSVEDKKAAALAELLSEDVGRQQAIADTVGDE
                     VAGSLDDLIVMLVSQQWRRPPSARNGMSVEVLIEMLPDGTITNASVTRSSGDSPFDNS
                     AVQAVRNVGRIAEMQQLDRATFDRLYRQRRAVFKPEDLGL"
     gene            complement(2855..3307)
                     /gene="tolR"
                     /locus_tag="VFF22_07545"
     CDS             complement(2855..3307)
                     /gene="tolR"
                     /locus_tag="VFF22_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003254760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HZX16703.1"
                     /translation="MARIRNRRKPVAEMNVVPYIDVMLVLLVIFMVTAPMLNQGVKVD
                     LPQVSSEVLPQDNNAQVLTISIKADKTYFWNMGTEVDVDTVQDQALTLEEMTRAVTAI
                     ISQSRATGKQVQVFVRGDKTVDYGTIMAAMGGLQQADVGNVGLITEAP"
     gene            complement(3341..4036)
                     /gene="tolQ"
                     /locus_tag="VFF22_07550"
     CDS             complement(3341..4036)
                     /gene="tolQ"
                     /locus_tag="VFF22_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HZX16704.1"
                     /translation="MEANAVDHMSMWSLISNASLVVQLVMLTLVAASVTSWVMIFQRT
                     ALLRAAKRALDNFEERFWSGIDLSKLYRQAGSNPDPDSGLEQIFRAGFKEFSRLRQQS
                     GVDPDAVMDAVARAMRVAISREEEKLEQSLPFLATVGSTSPYIGLFGTVWGIMNSFRG
                     LAQVQQATLATVAPGIAEALIATAIGLFAAIPAVIAYNRFAARGEMLIGRYYTFADEF
                     QAILHRKVHTSDD"
     gene            complement(4017..4491)
                     /gene="ybgC"
                     /locus_tag="VFF22_07555"
                     /pseudo
     CDS             complement(4017..4491)
                     /gene="ybgC"
                     /locus_tag="VFF22_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
     gene            complement(4544..5593)
                     /gene="ruvB"
                     /locus_tag="VFF22_07560"
     CDS             complement(4544..5593)
                     /gene="ruvB"
                     /locus_tag="VFF22_07560"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZX16705.1"
                     /translation="MLEADRLITAAPRDRDEQVDRAIRPLKLAEYIGQPSVREQMELF
                     IQAARGRSEALDHTLIFGPPGLGKTTLANIIAQEMNVSIKSTSGPVLERPGDLAALLT
                     NLEPGDVLFIDEIHRLSPIVEEVLYPAMEDFQLDIMIGEGPAARSIKLDLPPFTLVGA
                     TTRAGMLTNPLRDRFGIVQRLEFYNTEDLATIVMRSAGILGLSIEPHGAFEIARRARG
                     TPRIANRLLRRVRDFAEVRAQGDITREIADLALNLLDVDERGFDHQDRRLLLTMIEKF
                     DGGPVGVDNLAAAISEERHTIEDVLEPYLIQQGYIMRTPRGRVVTRHAYLHFGLNVPK
                     RMGELPTADLFGDDD"
     gene            complement(5593..6198)
                     /gene="ruvA"
                     /locus_tag="VFF22_07565"
     CDS             complement(5593..6198)
                     /gene="ruvA"
                     /locus_tag="VFF22_07565"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZX16706.1"
                     /translation="MIGRLRGTLAEKQPPHVIVDINGLGYELEVPMTTLYRLPSVGEP
                     LTLHTHLVVREDAHLLYGFFEKRERELFRELIRLNGVGPKLALALMSGLEVDELVRCV
                     QAQDTSVLVKIPGVGKKTAERLLVELKDRFKAWETVPGMSHLVVEPRGGSAVSSAEND
                     AVSALISLGYKPQEASRAVSAIKEDGLSSEDMIRRALKGMV"
     gene            complement(6222..6746)
                     /gene="ruvC"
                     /locus_tag="VFF22_07570"
     CDS             complement(6222..6746)
                     /gene="ruvC"
                     /locus_tag="VFF22_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZX16707.1"
                     /translation="MTLILGIDPGSRITGYGVVRDTGHNCEYVASGCIRTGNGSMPER
                     LQVVFRGVREVIETYGPVTMGIEQVFMARNPDSALKLGQARGVAIVAGIEAGLDIAEY
                     TATQVKQAIAGTGGADKEQVQMMVMHLLKLVQKPQIDASDALAIALCHAHHRQSLIPH
                     GLAGAKRRAGRLRL"
     gene            complement(6915..7664)
                     /locus_tag="VFF22_07575"
     CDS             complement(6915..7664)
                     /locus_tag="VFF22_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZX16708.1"
                     /translation="MAGHSKWANIKHRKGRQDAKRGKIFTKLIRELTVAAKHGGPLPA
                     DNPRLRLAVDKALTANMSRDVIDRAIARGAGNDEADNMIELTYEGYAPSGVAIFVEAM
                     TDNRNRTAAEVRHAFTKCGGNLGTDGSVAYMFDRKGQISFAPGVSEDALMEAALEAGA
                     DDVEMAEDGSALVSTSFADFHGVNEALSAAGFKADEAEIAMIPSINATVADLETAQKV
                     IKLIDMLEDLDDVQEVYHNAEISDELMEQLG"
     gene            complement(7800..9575)
                     /gene="aspS"
                     /locus_tag="VFF22_07580"
     CDS             complement(7800..9575)
                     /gene="aspS"
                     /locus_tag="VFF22_07580"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HZX16709.1"
                     /translation="MMRSHYCGQLNESLDGQEVTLCGWVHRRRDHGGVIFLDIRDREG
                     LAQVVFDPDRAATFATADKVRSEYVVKITGKVRLRPAGAGNANMASGAIEVLGYELEV
                     LNEAETPPFPLNEYTDVGEETRLRYRFIDLRRPEMAAKLKLRSSITSSIRRYLDDNGF
                     LDVETPILTRATPEGARDYLVPSRTHAGSFFALPQSPQLFKQLLMVAGFDRYYQIAKC
                     FRDEDLRADRQPEFTQIDIETSFLDEADIMAITEKMIRQLFKEVLDVEFGELPHMTLA
                     EAMRRFGSDKPDLRNPLELVDVADQLQAVDFKVFSGPANDPKCRVTALRVPGGASMPR
                     SKIDEYTKFVGIYGAKGLAYIKVNERAAGVEGLQSPIVKNIPLDNINVILDRVGAVDG
                     DIVFFGADKAKIVSEALGALRIKLGHDLNLLTCEWAPLWVVDFPMFEENDDGSLTAMH
                     HPFTAPKCSPAELEANPAAALSRAYDMVLNGTELGGGSIRIHDKSMQQTVFRVLGISE
                     DEQQEKFGFLLDALKFGAPPHGGLAFGLDRLVMLMTGAQSIREVIAFPKTQSAACVMT
                     QAPGEVDNKSLRELHIRLREQPKAE"
BASE COUNT         1962 a         2924 c         2726 g         1979 t
ORIGIN      
        1 gagcgccaaa acggttaaag cacgtcggca cgtacgcata tcgaattact tacgcagttc
       61 gacgcgacgg ttttgagccc aggaggactc gtcgttgcca gtggcgattg cgcgctcttc
      121 accgtaggaa accagttcca gttgagccgg ggaaacgccc tgcagtacca ggtaacgctg
      181 cacagctttg gcgcgacgct cgcccagagc catgttgtat tcacgagtac cgcgctcgtc
      241 agcgtggcct tccagaacta cgcgagcgcc attgccttgc aggtctttgg cgtgcacgtc
      301 cagagcgcgc atggcctcgg gtttcaggtc ggagctgtcg tattcgaagt agaaagtggt
      361 gatagcgcgc agagcagctt cttcgctcag gctgccatcg acagcgccag tgttggcacc
      421 gtaaccagcg ttaggatcgg tagcgccttc gccagcagcg tcgccgcctt tcgaggagca
      481 accaactgca acggccagag ccaggctcag cgcggcgaac ttaccaaatt tcagcatttc
      541 catcatgtaa ctcctaatga accccagtgt gttaagcagt atttcaaaca acaacggtgt
      601 agcgccgcat cagttcaggt aaggggacca agaaggctct cgaacttcgc cttgagcggt
      661 aggaagaggg agcctcacac gtccattggt ggacgctaat accaagactc cccgaccctg
      721 ctggcgggtg gcgtagatta ccatggtgcc gttaggcgca acagtaggcg actcatccag
      781 actggtatct gaaagtatcc gcaaattgcc gcgctgcagg tcttgcgccg ccactttgaa
      841 cacggtgtaa ccatcctggc ggtggatcat gaccaaggtc ttctcatcag cagagagttt
      901 cgggttagcg ttgtagttac cgataaatgt cacacgctct gccgagccac cattgatatt
      961 ggttttgtag atctgcggtt tgccggagcg atccgaggtg aagtacagag tctgaccgtc
     1021 tttaccccag aatggttcgg tatcaatcga tggctgagtc gtcacgcggc gcagctgacg
     1081 gctacccatg tccatcacgt agatttccgg gttgccatca cgcgacagca cgaaggccag
     1141 gcggttgcca tctggcgacc aggccggcgc accgttgaga ccttcaaaat tggtgatctg
     1201 ctcgcggcga ccggtatcga tgtgctgcac gaagatgcgc ggacgcttct gctcgaaaga
     1261 gacataggca atacgcttgc catccggggc aaacgatggc gacagaatcg gctcgcgcga
     1321 ttgcagcaag gtcaccgcac gggcgccgtc atagtcggaa cgctgcaggg tgtaacgggt
     1381 gttgctcacc gagaaacgct cagcggtgac gtaaagcatg cgagtggaaa acgcaccctt
     1441 gacgccggtg agcttctcat acgcctggtc agcgatatgg tgcgccatat cacgcagctg
     1501 atcgacacca ccaccgacac tgccagtcat gacttgctgt tgagtggtga cgttgaacaa
     1561 ggcgaactgg atctgcaggc gaccggcgtt cggcacaatg ctgccgacca acacgtactg
     1621 cgcgcccaga gcctgccagt cgcggtagat cacttcagtc gcctgagtcg gcaagctgat
     1681 catgttctgc cgaggaatcg gctcgaatac cccggagttg cgcaggtcat tgccgatgat
     1741 ttcagccatg tcctcgggta acacgttgcc gccttgccag ccaaaaggca ccacagcgat
     1801 gggtgtggca cggtcggtgc cactgctgat caccagcgga tcagcggcct gaacggtgcc
     1861 gaccatcagg gtcagaccga gcagaacaat acgcatcagg gtattcacag acctaaatcc
     1921 tccggtttga agacagcgcg acgctgccgg taaaggcgat caaaagtggc gcggtccagc
     1981 tgttgcattt cagcaatacg gccgacgtta cgcaccgctt gcacggccga gttatcaaat
     2041 ggcgagtcgc cactggagcg cgtcacgctg gcattggtaa tggtgccatc aggcagcatt
     2101 tcgatcagta cttctacgct catgccatta cgcgcagatg gcgggcgccg ccactgctgg
     2161 ctgaccagca taacaatcag atcatccaga ctgccggcga cttcatcacc gacggtatcg
     2221 gcgattgcct gctggcgacc gacatcctcg gacagcagct cggccagtgc ggcagccttc
     2281 ttgtcttcta cagatttacg ctgagccgct tctgccgcct ttttcttggc gtcatcagct
     2341 tttttcttct tcgcagcttc tgcagcggct ttcttcttcg cctcttctgc agcttttttc
     2401 ttggtgtctt cagcggcttt tttcttagcc gcctcatccg ccgctttctt tttggctaca
     2461 tcggcgagct tcttctgctc tgccgtcttc ttgtcggcgt cggccttttt ggcagcctcc
     2521 gctttctgag cctcttgagc tttcttttgt tccgctgcct tggccgctac tttttcctgc
     2581 tcaacttttt tctgctcaag ctgttcggtt tcgtacaccg gggcagtggt cttcttggtt
     2641 tcgccagcga ttttctgatt ggtctgtgtg gtggcctggc tctgcgactg caacttgtac
     2701 agcgtggcct gtaccaccgg cttggccggc ggtagatccg gggcaaaggc gaagctgacg
     2761 aacagcatgg caaacatgat gacgtgcagg cccacagccc aaaccacagg ccagaacaga
     2821 ctttccgagg gcgaacgctc gcgctggtag tgcatcaggg ggcctcggta atcagcccga
     2881 cgttacccac gtcagcctgc tgcaagccac ccatggccgc catgatggtg ccgtagtcaa
     2941 cggtcttgtc gccgcgcacg aacacctgca cctgcttacc ggttgcccgg ctctggctga
     3001 tgatcgcagt cactgcgcgg gtcatttctt ccagggtcag cgcctggtcc tgaacggtat
     3061 cgacatcaac ctcagtgccc atattccaga aataggtctt gtcggccttg atcgagatgg
     3121 tcagcacctg agcgttattg tcctgtggca agacctcact gctgacttgc ggcagatcga
     3181 ccttgacccc ttgattgagc atgggcgcgg tcaccataaa gataaccagc agtaccaaca
     3241 tcacatcgat gtaaggcacc acgttcatct cggcgacggg cttgcgtctg ttgcgaattc
     3301 tggccatggc ttaaaacctt tcgtaaagag tgcctaaggc ttagtcgtcg ctggtgtgga
     3361 ctttgcggtg caggatcgcc tggaattcat cggcgaaggt gtagtaacga ccgatcagca
     3421 tttcgccacg ggcggcaaaa cggttgtagg cgattaccgc aggaatggcg gcaaacaggc
     3481 cgatagcagt ggcgatcagc gcctcggcaa tacccggggc cacagtggcc agggtcgctt
     3541 gctgcacctg cgccaggccg cggaaggagt tcatgatgcc ccacacggta ccgaacaggc
     3601 cgatatacgg actggtcgaa ccgacagtgg cgaggaacgg caggctctgc tcaagcttct
     3661 cttcttcacg cgagatggcc acacgcatgg cacgagccac ggcatccatc accgcatccg
     3721 gatcaacacc ggactgctga cgcagacgcg agaactcttt gaagcctgct cggaagatct
     3781 gctccagacc ggaatctggg tccgggttgc tgcccgcctg gcggtacagc ttggacaggt
     3841 cgatacccga ccagaagcgt tcctcgaaat tatccagggc acgcttggcc gcacgcagca
     3901 gcgcggtgcg ctgaaaaatc atgacccacg aagtgaccga agcggccacc aacgtcagca
     3961 ttaccagttg caccaccagg ctggcgttgc tgatcagact ccacatcgac atgtggtcaa
     4021 cggcgttggc ttccacgctt actctcctgc tctagatgta cccgcgccgc cctgcccggc
     4081 aaaggccgtg tgcagagctt ggggaatggc ccggggtttc aaattgtcgg cgcgcacaca
     4141 ggccaccata aactgccctt cacaaagcag cgcatcatcc gttgcccgcc tgacctgttg
     4201 acgaaaacgc aggctggcac ggtttaattc aattacttca gcgcttacca gcaactcgtc
     4261 gtcgagcttg gctggcgcgt ggtagcgcgc ttcagccgaa tgcacaacga acaacaggcc
     4321 ctcctcggcc aacgtcgact ggagaaaacc cagatcacgc agacactcgg tgcgagcccg
     4381 ttccataaat ttgaggtaat tgacgtagta gacgatgccg cctgcatcgg tgtcttcgaa
     4441 ataaacccga caacgatggg tgaacggcgt aactccgttt tgcgcgcgca tactctagtg
     4501 ccagctcctg atcttgccaa tcgtcttcga caagtatttt ttgttaatca tcatccccga
     4561 acaggtcagc cgtggggagt tcgcccatgc gcttgggcac attcaggcca aagtgcaaat
     4621 aggcatgccg ggtgaccacg cgcccacgcg gggtgcgcat gatatagccc tgctgaatca
     4681 gatagggctc cagcacatct tcgatggtgt ggcgctcttc gctgatggca gcggccaggt
     4741 tatcgacacc caccgggcca ccgtcgaact tctcgatcat ggtcaacagt agacgtcggt
     4801 cctggtggtc gaagccacgc tcatcaacat ccagcaggtt caaagccagg tcggcgattt
     4861 cgcgggtgat atcgccctgc gcacgcactt cggcaaagtc gcgcacacgg cgcagcaaac
     4921 ggttggcgat acgcggcgta ccgcgggcgc gacgggcaat ctcgaaggcg ccatggggct
     4981 cgatcgacaa gccgagaatc cccgcagagc gcatgacgat ggtcgccagg tcttcggtat
     5041 tgtagaactc cagacgctgc acaatgccaa aacggtcgcg cagtggattg gtcagcatgc
     5101 cggcacgggt ggtcgcaccg accagggtaa acggcggcag atcgagcttg atcgaacgtg
     5161 cggccgggcc ttcgccgatc atgatatcga gctgaaaatc ttccattgcc gggtacagca
     5221 cttcctcgac aatcggcgac aggcgatgaa tctcatcgat aaacagcaca tcgcccggct
     5281 caagattggt cagcaaagcc gccagatcac ccggccgctc cagcaccgga cccgaggtgc
     5341 tcttgatcga aacattcatt tcctgagcga tgatattggc cagggtggtc ttgcccagac
     5401 cgggcgggcc gaagatcaga gtgtgatcga gcgcttcgct gcgcccacgg gccgcctgga
     5461 taaacagctc catctgctcg cgcacgctcg gctggccgat gtattcggcc agtttcagcg
     5521 ggcgaatggc acggtcaacc tgctcatcac ggtcacgcgg ggctgcggta atcaggcgat
     5581 cagcttctag cactagacca ttcccttcag ggctcgacga atcatatctt cactgctcaa
     5641 accgtcttcc ttaatagctg agacggcgcg actggcttcc tggggtttgt agccgagcga
     5701 aataagtgca ctgaccgcat cattctccgc gcttgagact gcactgccgc cgcgaggttc
     5761 caccaccagg tgcgacatgc cgggcacggt ttcccaggct ttgaagcgat ccttcagttc
     5821 aaccagcagg cgctcggcgg tttttttacc gacacccgga atcttcacca gcaccgaggt
     5881 gtcttgcgct tgcacacagc gcaccaactc atcgacctcc aacccggaca tcaaggccag
     5941 ggccagtttc gggccgacgc cattgaggcg aatcagctca cgaaacagct cgcgctcgcg
     6001 cttttcgaaa aaaccataaa gcagatgcgc atcctcacgc accacaagat gggtgtgcag
     6061 ggtcaacggc tcacccaccg agggcaaacg gtacagggtg gtcatgggca cttccagctc
     6121 ataacccaaa ccatttatgt ccacaatcac atggggcggc tgcttttccg ccagggtgcc
     6181 gcgcaaacgt ccgatcaccg ctgctttcct taaaaattac gttacaaacg aaggcgaccc
     6241 gcgcgccgct tggccccggc caagccatgg ggaatcagac tctgccggtg gtgggcatgg
     6301 cacagggcaa tggccagggc atcggaggca tcgatctgcg gtttctgcac cagcttgagc
     6361 aggtgcatga ccatcatctg cacctgctcc ttatccgccc cgcccgtacc ggcaatcgct
     6421 tgtttgacct gggtcgcggt gtattcggcg atatccagcc ccgcttcgat ccccgcgaca
     6481 atggccaccc cgcgcgcctg gccaagcttg agcgcggaat ccggattgcg cgccataaac
     6541 acctgctcga tgcccatggt caccggcccg taggtttcga tgacctcacg cacgccgcga
     6601 aacaccacct gcagacgctc aggcatcgaa ccattgcccg tgcgaatgca gccggaggca
     6661 acatattcgc aattgtgccc ggtatcacgc accacaccgt aaccggtgat gcgcgaacca
     6721 gggtcgatgc caagaatcaa ggtcatgccc taccgcccac tcaaggcacc agccaactca
     6781 gccggcacac tgtctattta tccagttagc gagtataggg ccagcccact tcggcgtcac
     6841 ccaggcacac aaaaaagccg gacgcttcgg gcaaataacc cgaatacctc cggctgctgt
     6901 agatacccag acacttagcc aagctgctcc atcagctcat cggagatctc ggcgttgtgg
     6961 taaacctcct gcacatcatc aaggtcttcg agcatatcga tcagcttgat gaccttttgc
     7021 gcggtttcca gatcggcaac cgtcgcattg atcgatggga tcatggcgat ttccgcttca
     7081 tcggccttga aaccggccgc gctgagcgcc tcgttgaccc catggaaatc ggcaaagctg
     7141 gtcgacacca aggccgagcc atcctcagcc atttccacgt catcagctcc ggcttccagc
     7201 gccgcttcca tcagggcatc ttcgctgacg cctggggcga agctgatttg gcccttgcga
     7261 tcaaacatat aagccaccga accatcggta cccaggttgc cgccgcactt ggtaaaggca
     7321 tggcgtactt cggctgccgt gcgattgcgg ttatcggtca tggcctcgac gaagatcgcc
     7381 acgccgcttg gcgcgtatcc ttcataggtc agctcgatca tgttgtcggc ttcgtcgtta
     7441 cccgcccccc gggcgatggc acggtcgata acgtcgcgcg acatgttggc cgtcagcgcc
     7501 ttgtccaccg ccaggcgcag acgcgggttg tcggccggga gcggcccgcc atgcttggca
     7561 gcaacggtca gctcacgaat cagcttggtg aagatcttgc cgcgcttggc gtcctgacgc
     7621 cctttgcggt gcttgatgtt ggcccacttg gaatgaccag ccataactca ctccgtttgt
     7681 ctcagttcaa ttcactgtag gagcgggcca tgcccgcgaa agaaagatcg cggccatggg
     7741 ccgctcctac gggataccta taaagaagaa gcctggaaaa ccaggcttct tttgacgctt
     7801 tattccgctt tcggctgctc gcgcagacga atatgcaatt cgcgcagcga cttgttatcc
     7861 acctcacccg gcgcctgagt cataacgcag gccgcgctct gggttttcgg gaaggcgatc
     7921 acttcgcgga tcgactgggc accggtcatc agcattacca aacgatccag gccgaaggcc
     7981 agaccgccat ggggcggcgc gccgaacttg agggcgtcga ggaggaagcc gaacttctcc
     8041 tgctgctcgt cttcgctgat acccagcacg cggaacacag tctgctgcat cgacttgtcg
     8101 tggatacgga tcgaaccgcc acccagctcg gtgccgttaa gcaccatgtc ataggcacgc
     8161 gacagcgcgg cggccgggtt ggcttccagc tcggccgggc tgcacttcgg tgcggtgaac
     8221 gggtggtgca tggcggtcag gctgccgtcg tcgttctctt cgaacatcgg gaagtcgacg
     8281 acccacagcg gcgcccactc gcacgtcagc aggttgaggt cgtgacccag cttgatgcgc
     8341 agcgcgccca gggcttcgct gacgatcttg gccttgtcgg caccgaagaa cacgatgtca
     8401 ccatcgaccg caccaacgcg atcgaggatc acattgatat tgtccagcgg gatattcttg
     8461 acgatcggcg actgcaggcc ttcaacaccg gcagcacgct cgttgacctt gatgtaagcc
     8521 aggcccttgg caccgtagat gccaacaaac ttggtgtatt cgtcgatctt gctgcgcggc
     8581 atgctcgccc cgcccggtac acgcagcgcg gtaacgcggc acttcggatc gttggctgga
     8641 ccgctgaaca ctttgaagtc gaccgcttgc agctggtcgg ccacgtccac caattccagc
     8701 gggttacgca gatccggctt gtccgaacca aagcgacgca tggcttcggc cagggtcatg
     8761 tgcggcagct cgccgaactc gacgtccagc acttccttga acagctggcg aatcatcttc
     8821 tcggtgatgg ccatgatgtc ggcttcatcg aggaagctgg tttcgatgtc gatctgggtg
     8881 aattccggct ggcggtcggc acgcaggtct tcgtcgcgga agcacttggc gatctggtag
     8941 tagcggtcga agccggcgac catcagcaat tgcttgaaca gctgcggcga ttgcggcaaa
     9001 gcgaagaagc tgccggcatg agtgcggctc ggcaccagat agtcacgcgc gccttcaggg
     9061 gtggcacggg tgaggatcgg cgtttccacg tcgaggaagc cattgtcatc taagtagcga
     9121 cggatgctgc tggtgatgct cgaacgcagc ttcagcttgg ccgccatttc cgggcgacgc
     9181 aggtctataa agcggtagcg caggcgggtt tcttcgccca cgtcggtgta ttcgttgagc
     9241 gggaacggcg gggtttccgc ttcgttcagc acttccagct catagcccag cacttcgata
     9301 gcgccggacg ccatattggc gttgccggca ccggccggac gcagacgcac cttgccggtg
     9361 atcttgacca catattcgct gcgcaccttg tcagcggtgg cgaaggtcgc cgcgcgatcc
     9421 gggtcgaata ccacctgagc cagaccttca cggtcacgaa tgtcgaggaa aatcaccccg
     9481 ccatggtcac gacggcgatg tacccaaccg caaagggtga cttcctggcc gtccaggctc
     9541 tcgttcagtt ggccgcaata atggctgcgc atcatggtgg tggtcgcttc t
//
LOCUS       DASRUM010000113        31078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2067075, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000113 DASRUM010000000
VERSION     DASRUM010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 31078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31078
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067075"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            466..2433
                     /locus_tag="VFF22_07585"
     CDS             466..2433
                     /locus_tag="VFF22_07585"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX16710.1"
                     /translation="MNIKSKLLVSFLVATLIPVLVVALLTIRNVTQQAKENFLVSSSL
                     DMKLVNNSFATFFDSVGYTVSALAEYPAVKDAENGELTTYFGEARKPGEVATANGGRE
                     KQIFDYFSSIGKNNPTFGYVYMSDTKGGYVEWPGTGDYGDWDPRKREWYPIGRDANFT
                     LGRRDGYYWEPDDAVYVSVLKGFKDNAGQFAGVVAVDVSLKALTDMAQKVRFGETGFL
                     MLAEGSGTLLVDGLQPDNNFKKVSELPGEHFATIGKTENGLVEVEIDGVAYMANVYTS
                     PELGWKFVGFKQADEIYASARELTWITLIVCIVLVLVFGAAGVVIANRIVNPINLVKD
                     GLRTIAQGEGDLTRRLDLLSKDETGELAKWFNQFIESIQGMIKVIKENAISMDGVSSD
                     TNKRTTAMSATLQRQLSSVEQIVTAVTEMSSAANEVAKTCVQTADVSEQGLMATRNGK
                     EVIARSTSGVNKLGASIQNSSKVLQELEKETVNINNILSTIQQIAEQTNLLALNAAIE
                     AARAGEQGRGFAVVADEVRNLAKRTQDSTEQINSILNLLVNRIKEVTVSMDQSLVESG
                     KAIVLSDEVMAAFESIEGKVQMIRDMTMQIATATEEQHLVTEEINQNIVAINDAVTQI
                     SVQAAEVESYAQEQSGLSSELKQLVVRFRTE"
     gene            complement(2489..2845)
                     /locus_tag="VFF22_07590"
     CDS             complement(2489..2845)
                     /locus_tag="VFF22_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011062290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16711.1"
                     /translation="MLSRIPLITGLVASLALFAQAPAQASNRDAAIAGAVVGAVIGGV
                     IVSQTRPVQVYAPPPREYYPPPPAYYPPQRVYYERQPVYYQPAPVYYVQERPRHKRWH
                     GHKHRRHDGHGHGHRW"
     gene            3111..4202
                     /locus_tag="VFF22_07595"
     CDS             3111..4202
                     /locus_tag="VFF22_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003185431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16712.1"
                     /translation="MLVAVAWLLACHAQAADVTVKLGSTERVTRLFAYPNNCNVICYR
                     DWTLEQTAEHYLRQSLQRDGYDKATVTVHREEQELFAKFSEVPEGYGKPLEQLLKTGE
                     LAYSGAKLLNKDGKWQYNWSLFLPLGMALDNRKSVELLHFPPDYSLTQAQDYLESSTT
                     NRWADLLTENGISAAQTPAFQTIIDIAPIAAPANAGSALEGVYSYFNAYQTQMVMQLT
                     AGEGGQALPMVAFGSPVRSWVNQQYGVSIDVLGLAQISPAAGQQVAVLGANHPSYIWY
                     AADPENYDGDQQKADAAGLKVMGQDLSAACWQASMGEQPSQDASPVLSACSEKWQVTE
                     KLQTCELFYTSVRNLTPEAAQAKCTPASS"
     gene            complement(4250..4894)
                     /locus_tag="VFF22_07600"
     CDS             complement(4250..4894)
                     /locus_tag="VFF22_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HZX16713.1"
                     /translation="MPSTATPSAAHAIAETRAWVDRAVIGLNLCPFAKAVQVKGQVRY
                     VVCASAEPEELLAMLIDELHHLANCEPSETDTTLLIHPAALTDFLDFNDFLAVADDAL
                     DDLGYSGILQVASFHPQFQFADTHAADMGNATNRSPYPTLHILREASIDRAVAAFPDP
                     ETIFEANIQTLEELGADGWATLQAQCRKDAQNQVPAVTPEVSAATAGNQTPAIP"
     gene            complement(4974..5699)
                     /locus_tag="VFF22_07605"
     CDS             complement(4974..5699)
                     /locus_tag="VFF22_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HZX16714.1"
                     /translation="MLRLLALFCLLVALPAFADAPKTFSAAKKIAWRLYAERPNTFYC
                     QCAYSGNRVDLGSCGYKVRKQERRAARVEWEHVVPAWVIGHQRQCWQNGGRKNCTRTD
                     ASFKAAEADLHNLVPSIGEVNGDRSNYALGMLSDKPTQYGSCPMVVNFKAKTAMPPVH
                     ARGAAARIYLYMAERYQLRLSKLDRRTYEAWSRQYPISEWERWRNQQVGCVMGHGNPY
                     IGAIDPSQCNPLRRAGNPQRNSG"
     gene            6017..6727
                     /locus_tag="VFF22_07610"
     CDS             6017..6727
                     /locus_tag="VFF22_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007160409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16715.1"
                     /translation="MRLLVLIVLLLAVSGCSTRQDHQTIISNRSAIKPSEFFQSHADR
                     MATIGMRNNLNSLYRLMDKLYLRNPREWRKTGLADVEAAKRAVHRSIELDQPLPQLGG
                     RTDVAALGYALSAEYQGDRVGAFIYSLASMLITAHGGRTEYYLSDTLNPQFVHNAARN
                     FEKASWMLAQRTDASGKPWLLSNEISAEGHNLSYAVEFGKIVARLDLLTDVLDERYRR
                     MGVSYAQGLLFLNFLPVQ"
     gene            complement(6746..7804)
                     /locus_tag="VFF22_07615"
     CDS             complement(6746..7804)
                     /locus_tag="VFF22_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016969772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HZX16716.1"
                     /translation="MPDSASPADLPRDLHYVDDSQPGIHRKKVRGTFNYFDAKGERIT
                     DADEIKRLDSLAVPPAYTDVWICADPRGHLQATGRDARGRKQYRYHPRWREVRDTDKY
                     ARLQAFGNALPKLRQQLQAQIATPGFTREKVLATVVMLLDATLIRVGNSQYARDNKSY
                     GLTTLRTRHVAIQGSDIKFQFVGKSGIEHQVSVKDRRLATVVRRCMELPGQNLFQYLD
                     EDGARHTISSTDVNAYLHQLTGADFTAKDYRTWAGTAMALAVLRELEWQPESDAKRHV
                     VATVKAVAKQLGNTPAVCRKCYIHPAVLEHFSLGALSKLPKPRVRKGLKAEEVALAMF
                     LERLAADVPNTAKGRHRD"
     gene            complement(7979..8167)
                     /locus_tag="VFF22_07620"
     CDS             complement(7979..8167)
                     /locus_tag="VFF22_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16717.1"
                     /translation="MRVTLALVLLATTLSGCGLGETAATTSLQAEQAAQLQQQTEQLK
                     QQIEAANAQSNKRLEEGY"
     gene            complement(8202..8924)
                     /locus_tag="VFF22_07625"
     CDS             complement(8202..8924)
                     /locus_tag="VFF22_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX16718.1"
                     /translation="MRIAALCLMLLLPWAVQAEPWKVVGDEQFAPYSFVTIEDDQPRG
                     LDVELVEAVLQEAKIPYSLRLYPWERVKRMIDRSEVAMAFQFAGTPERMQQYELVGPI
                     RTGSTVFMTSRKITLNDWAELDDLSPYIIGQVRGYAYQSDFDKAELSRDTSAQNPRQL
                     VSMLLAGRIDVIVGDRVQLMYFVREQRAEKNVHILPKPLVEMPRYVAFAKGDSGRAKQ
                     FAAALERLRQAGTLESIYKRWQ"
     gene            complement(9038..9379)
                     /locus_tag="VFF22_07630"
     CDS             complement(9038..9379)
                     /locus_tag="VFF22_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HZX16719.1"
                     /translation="MSTLPPCPKCNSEYTYEDGAQLVCPECAHEWSATGASDAADDAK
                     VIKDSVGNMLQDGDTVTVIKDLKVKGSSLVVKVGTKVKGIRLCDGDHDIDCKIDGIGA
                     MKLKSEFVRKV"
     gene            complement(9430..9924)
                     /locus_tag="VFF22_07635"
     CDS             complement(9430..9924)
                     /locus_tag="VFF22_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HZX16720.1"
                     /translation="MPNALHRTLVRALLVSLLLTLTACSTLAPRDPLRIELVGIEPLP
                     GEGLEARFAIKLRVQNPNDQPIDFDGVDLELQVNQQPLLSGVSDQRGQVPRYGESILR
                     IPVSLSAYAALRQAWGMAGYQDGQGLPYELRGKLANGLFGTWRFNDRGTLNWPQPPAP
                     GAAR"
     gene            complement(10076..10870)
                     /locus_tag="VFF22_07640"
     CDS             complement(10076..10870)
                     /locus_tag="VFF22_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007898835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HZX16721.1"
                     /translation="MNRTLSSLVLAGGLLAGGQAVAGDLLHWQNNSLTYLYGQDYKID
                     PEIQQTITFEHASGWAIGDLFFFVDTIKYNTDATNGAGDGHTFYGEFSPRLSFGKLFN
                     TKLEFGPIKDVLLASTYEFGEDDVDSYLIGPGFDLAIPGFDYFSLNFYNRTTDGKRDG
                     DNVWQITPVWSYTIPVGKSDIVIDGFMDWVVDNDKSYHANLHFNPQIKYDLGKAMNWG
                     EKQLYVGIEYDYWKDKYGIDDNSFLGDEILQGTDQNTASLLVKAHF"
     gene            complement(11134..11415)
                     /locus_tag="VFF22_07645"
     CDS             complement(11134..11415)
                     /locus_tag="VFF22_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16722.1"
                     /translation="MTAKEAQLTELLSLSTRTLTHLTAAITAMSFELLNTQDSAVNAT
                     AHKMIERMLAINSELDQQWQLLGQLAGTQQDPGNALEQVVLKGVETLEN"
     gene            11981..15922
                     /gene="putA"
                     /locus_tag="VFF22_07650"
     CDS             11981..15922
                     /gene="putA"
                     /locus_tag="VFF22_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013692109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HZX16723.1"
                     /translation="MATTTLGVKLDDATRDRLKQAAQSIDRTPHWLIKQAIFNYLEQI
                     EGGLTPAEHSGLAAVAGEESVDSLTEQGLQVFLDFAESILPQSVLRAAITGAYRRPET
                     EAVPMLLEQARLPKDMAEASNTLAMKIAEKLRNQKNAGGRQGLVQGLLQEFSLSSQEG
                     VALMCLAEALLRIPDKATRDALIRDKISNGNWSQHLGNSPSMFVNAASWGLLITGKLV
                     ATHNEAGLVTSLNRIIGKGGEPLIRKGVDMSMRLMGEQFVTGETIAEALANATNMESK
                     GFRYSYDMLGEAALTDEDAKRYLASYEQAIHAIGKASHGRGIYEGPGISIKLSALHPR
                     YSRAQYERVMDELYPILLGLTKLAKHYDIGLNIDAEEADRLEISLDLLERLCFDPALT
                     GWNGIGFVIQAYQKRCPYVIDYVIDLAKRSRHRLMIRLVKGAYWDSEIKRAQVEGLEG
                     YPVYTRKPYTDISYIACARKLLAVPEAIYPQFATHNAHSLSAIYQLAGQNYYPGQYEF
                     QCLHGMGEPLYEQVVGKVADGKLNRPCRIYAPVGSHETLLAYLVRRLLENGANTSFVN
                     RIADTSISIKELVLDPVLQVEQMATQEGGLGLPHPRIALPRALYGDARLNSEGIDLAN
                     EHRLGSLSSALLSSTNTVYKAMPLLGCETPELSDAQPVRNPADHRDIVGHVHEASEQD
                     VRNAVLCAVSSGQIWQSTLPAERAAVLDRAADQMEAEMQQLMGLLVRESGKTFANAIA
                     EVREAVDFLRYYAAQARNHFSNDSHRPLGPVVCISPWNFPLAIFSGQVAAALAAGNTV
                     LAKPAEQTPLIAAQAVRILLDAGVPSGAVQLLPGRGETVGAGLIGNERVRGVMFTGST
                     EVAGIIQRNLAGRLDAQGRTIPLIAETGGLNSMIVDSSALTEQVVVDVVASAFDSAGQ
                     RCSALRVLCVQEDVADRVINMLKGAMAEYSLGNPERLSTDIGPVIDAEAKDNIDQHIE
                     KMREKGRKVFQTARAVGDDIKRGTFVLPTLIELDSFDEMQREIFGPVLHVVRYQRADL
                     GKLLQQINDSGYGLTLGVHTRIDETIAQVVNTAKVGNLYVNRNMVGAVVGVQPFGGEG
                     LSGTGPKAGGPLYMYRLLATRPQDAVAKQLQQENGEITRPAEQGKVIQALQDWAAKHE
                     PSLTALSAQYVELAQSGTVQMLNGPTGERNSYSLLPREHVLCLADERGDLLVQLAAAL
                     AVGCQVLWQEGELTRELFAALPKAVQQRITVLSQWSESETVFDAIIHHGDSDQLREVC
                     QLAAQRPGAIIGVHGLHKGETDIPLERLLIEHALSINTAAAGGNASLMTIG"
     gene            16316..16861
                     /locus_tag="VFF22_07655"
     CDS             16316..16861
                     /locus_tag="VFF22_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HZX16724.1"
                     /translation="MRLLTAVLIVGLSVSAQADNLQALTAEGMALIPPFQQQLMGTVK
                     AAMSSGGAAHAVEACQLSAPQIASEHSQKPWTVGRTALRVRNPDNAPDAWEREVLAQF
                     ARRAAAGEPLEQMSQSGVVAGELRVMKAIGAAEPCLACHGKQIEPALAQLIEVRYPQD
                     QARGFAQGELRGAFTLRRPVD"
     gene            16866..17132
                     /locus_tag="VFF22_07660"
     CDS             16866..17132
                     /locus_tag="VFF22_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HZX16725.1"
                     /translation="MNDQRLQHRDALLKRLARVEGQVRGIQAMIKRGDECESVAQQFS
                     AARKALDKAYQEMLMCLAEDAIRSPEQSHEATLAQLKTIFTKYT"
     gene            17369..18769
                     /locus_tag="VFF22_07665"
     CDS             17369..18769
                     /locus_tag="VFF22_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HZX16726.1"
                     /translation="MELDPLFLSRLQFAFVVCFHAIFPVFTIGLASFIAFLEGMSFRT
                     ADPVWARLSKFWTQVFAVVFGMGVVSGIVMAFQFGTNWSNFSYAASNFLGPVLSYEVI
                     TAFFLEAAFLGVLLFGRDKVPPGVHLFAACMVALGTFISSFWILSANSWMQTPVGTEL
                     RDGMIHVVSWSQALFSPSFIYRFMHMALASFLTGAFVVAGVSAWYLLRKREVEVNRKA
                     LSVSLWMILLIAPTQAVVGDYHGLNTLQHQPTKVAAMEGNWERASGVPLLLFAWPDQQ
                     AQTNHFEIAIPKLASFILTHDWDGELPGISEVPYEEQPPVWIVFWSFRVMVGIGLLMI
                     AFGLTGLLLRRGGRYWQAPWFLNGMRWMSFTPFLAVLAGWVVTETGRAPWLIYGVMTH
                     AQGLTPSLTGGMALFTLIGYIVVYAMVFSAGLYYLFRVLQAGLEEDLSAEDTHEVERA
                     SRPLSATHVRLEQGGH"
     gene            18774..19772
                     /gene="cydB"
                     /locus_tag="VFF22_07670"
     CDS             18774..19772
                     /gene="cydB"
                     /locus_tag="VFF22_07670"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HZX16727.1"
                     /translation="MPFDLTMIWAVIIAFGIIMYVLMDGFDLGVGILFPFAPDEQARD
                     VMMNSVAPVWDGNETWLVLGGAGLLAAFPLVYSVLLPAMYIGVFLLLAGLIFRGVAFE
                     FRFKANTSRYLWNWAFAGGSIIASFAQGAVVGAYIQGFNTEGLVYVGGALDWLTPFTV
                     LTGIGLLAGYALLGCTWLIMKSEGYIQDWAYSLAPKLLAAVLLVFVAISIWTPWVDPS
                     AYARWFSAIGLIWIFPVMAALCALQIFRSLRKRREAMPFIATMGLFIFTYLGLLVTKW
                     PYIIPPNHTIWDAASAPESQLFLLLGLLFVIPIVLTYTAWTYWVFRGKVKAGVGYH"
     gene            19756..21453
                     /gene="cydD"
                     /locus_tag="VFF22_07675"
     CDS             19756..21453
                     /gene="cydD"
                     /locus_tag="VFF22_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HZX16728.1"
                     /translation="MSAITDKTRKQRSRHWLTRQQQAVRPLLLGAVAAGVLGGVAIAA
                     QMGLMAWLVHALLIDAAPALSLWPVMSALLLAVLLRVTAAAVQERLGQLASAKVRQQV
                     RVELSALWARLGPVRLAELPSASLTNQWVEQVDALDGYYARYLPQQWLAALVPLMLVA
                     LVFWLDWLAALFLLLAAPLIPLFMALVGMHAERISQRHVLQAGRMAGQFLDRVRNLTS
                     LQLFGQVGRSITRIEQATQAYRTVTMATLKVAFLSSAVLEFFASVAIAVVAMYIGFGL
                     LGYIDYGPSPELSLCSGLFILLLAPEFFQPLRSLAQYYHDRAAALAAADALAELEALD
                     SLPAAPEDVPNHAAEPGIQLRDVHLSYVGRGEILRGVNLHINAGEVLALVGPSGSGKS
                     SLLHGLAGFIQPQAGTIWLFGQPPGQPPGQQAVAWMGQRPFLIKGSWAENLRLIAPEA
                     DASAMQQALASVGLSALLAAQPQGLDSPLGEGGRGLSGGQAQRLALARVWLSSAQLVL
                     LDEPTASLDEQSEAEVIKALQALASSGRTLIIATHHPALMHMANRVVRLHDGRLLDA"
     gene            21446..23110
                     /gene="cydC"
                     /locus_tag="VFF22_07680"
     CDS             21446..23110
                     /gene="cydC"
                     /locus_tag="VFF22_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HZX16729.1"
                     /translation="MHELSPWLRDMRYWRGRLCVGALLMLLTVLSAIGLLALSGWFIT
                     ATGVTALLWAAGNRVFFDIYVPGSGIRFFALARTVARYTERVFNHNTVLGLLADLRVR
                     HFSALAQLDGATLGRLRAAQWLNRLTADIDALDTLYLRLLAPPAVALLATLLVCGLVA
                     LFHPWLALLLLLLLLALLLLVTVGMARAGLHLSAQRVVQLDSLRVQCIEQLQGLAELT
                     VAGSLGLHQQALLARSEQLINDQLALQGRVVLGQALSLLGVLGASLLGLFAGVIAYQQ
                     GLLSGPLMVMIALALMGLNESFSALPAAFAQWGATCAAAARLNQQVAMSGRLPHAASP
                     VAVPANLTLSWQDVAVIHAGLSGVQLQLAAGERLALVGPSGCGKSTLAALAARLLDPD
                     AGAVCVAGVPLTALELDAWRGQIAYLTQETELLHGSIADNLLLGCPDASDAQLWWALD
                     MVDLADTVESTANGLNTWVGETGKQLSGGEGRRLALARVLLRDAPLVILDEPFSGLDE
                     ATRERIKPRLNAWLQGRSALLLGHSEAALPQADRLLSWQQLPRVPR"
     gene            complement(23227..23607)
                     /locus_tag="VFF22_07685"
     CDS             complement(23227..23607)
                     /locus_tag="VFF22_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZX16730.1"
                     /translation="MKSAHDLVSQAKTRIEEVDLHAADAAIRTADVLIDVREADEYQA
                     GHIPGAVNIPRGLLEFKLSNDPALTRRDLNLVLYCKNSGRSALAASTLQDMGYGNVLS
                     ITGGYEAWHAVGKPVQTPQLPAFD"
     gene            complement(23607..25352)
                     /gene="sulP"
                     /locus_tag="VFF22_07690"
     CDS             complement(23607..25352)
                     /gene="sulP"
                     /locus_tag="VFF22_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HZX16731.1"
                     /translation="MQLSRWLPSLDWGRHYSRRNASQDGLAALIVTLMLIPQSLAYAM
                     LAGLPPVMGLYASILPLLAYALFGSSRTLAVGPVAVVSLMTAAALQPLFPAGSAEYIG
                     AAMLLALLSGLLLSAMAVLRLGFLANFLSHPVVSGFISASGILIAIGQLKHLLGISAS
                     GENLLHLLTQLWLGLSAVHEPTLLIGLLSLAWLWWARAHLKGALQRLGIPAAAAGNLT
                     KAGPVLAIIAAIVAVSALQLDHAGVKVVGAIPQGLPGLTLPTLNLDLALQLLPAALLI
                     SLVGFVESVSVGQTLAAKRRQRIQPNNELLGLGSANLASAFSGGFPVTGGFARSVVNY
                     DAGAQTPMAGVFTAAGIALSVLLLTPLLHNLPHAVLAATIIVAVLSLVDLGSLRRTWR
                     YSRQDGAAQVATLLGVLLIGVEAGILLGVGLSLLLFLWRTSQPHIAVVGQVPGSEHFR
                     NVERFAVIESPSVLSLRVDESLYFPNARYLEDRIGELIASRPEVRHLVLMCSGVNLID
                     ASALDSLEAIVERLQAAGVQLHLSEVKGPVMDQLRRSSFLDTFAGQVFISQFDALKTL
                     APQTTQPSPIANRMP"
     gene            complement(25402..26265)
                     /locus_tag="VFF22_07695"
     CDS             complement(25402..26265)
                     /locus_tag="VFF22_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZX16732.1"
                     /translation="MQPHVEALFDPATFTYSYVVSDPSTQRCAIIDSVLDYDPASGRT
                     SHSSADQLIDYVKAHNLQVDWLLETHVHADHLSAAPYLKRELGGQLAIGEHITTVQNT
                     FGKLFNAGSEFATDGRQFDHLFKEGDTFAIGSIQARAIHTPGHTPACMTYVIGDAGFV
                     GDTLFMPDYGTARCDFPGGDARTLFQSIRKLFALPDDTRLFMCHDYKAPGRDDFRYQT
                     TVAEQRALNVHVHEGISEADFVAMRNARDSTLDMPMLILPSVQVNMRAGQLPPAEDNG
                     TRYLKIPLDVL"
     gene            26477..26809
                     /locus_tag="VFF22_07700"
     CDS             26477..26809
                     /locus_tag="VFF22_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZX16733.1"
                     /translation="MFSTPRYISNLGAQYQPHQDWQFGLQARAQGDYYIDELNAQGKY
                     GGFAVLDANVRYNLSATTSLDLQMKNVLDEEHEYVWYDNFFWGGDDQPMFSAAPGRSA
                     YLSVNMKL"
     gene            complement(26919..29093)
                     /gene="katG"
                     /locus_tag="VFF22_07705"
     CDS             complement(26919..29093)
                     /gene="katG"
                     /locus_tag="VFF22_07705"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HZX16734.1"
                     /translation="MDPKSQSTAGQCPVMHGANTANDQSSVAWWPNALNLDILHQHDS
                     KSNPLGQGFNYREELKKLDAAALKRDLTALMTNSQDWWPADWGHYGGLMIRLTWHAAG
                     TYRIADGRGGAGSGSQRFAPLNSWPDNANLDKARRLLWPIKKKYGNCVSWADLLALAG
                     TIAYESMGLKTFGFAFGREDIWHPEKDIYWGSEKQWLAPTGSEGSRYSGERDLANPLA
                     AVMMGLIYVNPEGVDGKSDPLKTAHDVRITFARMAMNDEETVALTAGGHTVGKAHGNG
                     NAKNLGPEPEGADIEEQGLGWNNHVSRGVGRDTVTSGIEGAWTTNPTQWDNGYFHLLL
                     NYNWELKKSPAGATQWEPINIKEEDKPVDVEDPSIRLNPIMTDADMAMKMDPEYRKIS
                     ERFYKDQAYFSDVFARAWFKLTHRDMGPKARYVGPDVPAEDLIWQDPVPAGRSDYNVA
                     AVAAKIAASGLSISERVSTAWDSARTFRHSDKRGGANGARIRLAPQKDWDGNEPARLA
                     KVLAVYEKIASETGASVADVIVLGGNLAIEEAAKAAGVTVKVPFAAGRGDASQAQTDV
                     ESFEVLEPLHDGFRNWQKQHYVVQPEEMLLDRAQLMGLTAPEMTALIGGMRVLGTNHG
                     DSQHGVFTDRVGVLSNDFFVNLTDMAYNWKPTGRNSYAIVERKSGATKWTATRVDLVF
                     GSNSILRAYAEVYAQDDSREKFVHDFVAAWSKVMNADRFDLL"
     gene            complement(29357..30124)
                     /gene="phnE"
                     /locus_tag="VFF22_07710"
     CDS             complement(29357..30124)
                     /gene="phnE"
                     /locus_tag="VFF22_07710"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HZX16735.1"
                     /translation="MNRLINLLLLLGIGAAVIASFVYLGIDLGALTSGDSLGQMAGYV
                     RRFLSPDLSAEHLAAIGRGALETLAMSALGTLLAAVFGLLLALPAAGRFGWPLQSAAR
                     LLLNALRAIPELVWAALMVLAAGLGPNAGTLALALHTAGVLGRLFAEALENTPTAPAE
                     AIRLQGGSQVAAFCYGTLPNLWPQLLAYCLYRWENNIRMASVLGFVGAGGLGQMLYIS
                     LSLFQESQASTVIIAMLLLVFGVDALSSWSRQRWIRA"
     gene            complement(30121..30939)
                     /locus_tag="VFF22_07715"
     CDS             complement(30121..30939)
                     /locus_tag="VFF22_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZX16736.1"
                     /translation="MLSRDPAALPRLLLTLLALALLWPGIQLSELDLSVLLQADSQSE
                     MGRFVAAFWPPAHDADFLQLLLDATLQTLAIATAGMALALLLAIPASLLASRALSLSA
                     ASRGGRPKPLAQLLRWPVRGLLIFLRSVPEIVWALLFVRAVGLGPTAGVLAIAITYSG
                     MLGKVYAEIFESVDQRPAHALLQSGSGRLAAFVYGILPNAAAELTSYTVYRWECAIRA
                     SVVMGFVGAGGLGQQIDLSMRMFAGGEVASMLLTFLLLVLLADQLSRVLRGRLT"
     gene            complement(30933..>31078)
                     /locus_tag="VFF22_07720"
     CDS             complement(30933..>31078)
                     /locus_tag="VFF22_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008073187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphonate ABC transporter"
                     /protein_id="HZX16737.1"
                     /translation="GLRDGQILFDRPAGEVDQAQLDALYANEQLLSPPAPSVTLSLQI
                     PRC"
BASE COUNT         5661 a         9125 c         9968 g         6324 t
ORIGIN      
        1 ggaggccttc ccctctccct aaccctctcc ccggaggggc gaggggatga tcgcgtccac
       61 ccgatgctta agtgaacagc attacgcaac tgtggggact ttttgtttac ctgcgatagc
      121 cgcgatgttt ttactttttt gtttttgccg tccacacatc gtgtaagcaa cgcggttccc
      181 gagccccatc aggaggccta aacgacgcgc cgtaacagcc ctaggctggc ccgaggggtg
      241 agcgcagcga atcaagtaac tcgccgtaag ggcgaaaccc ttaatctgcg tcaccgctgc
      301 ggcggcggaa tgcctgcggt caaccagcgg ccgattgacc gtgacagtcg cttttgctcg
      361 gcagcgttgt cgtggtgagt cccacgctgg cgctgtcata agccttaact atcctcaaca
      421 cgcgccgata gagcgtatta ctggtcagct agaaggcgaa gtcccatgaa tatcaaatcc
      481 aaactgttgg tgagctttct ggttgcaacc ctgattccgg ttctggtggt ggcgctgctg
      541 acgattcgca atgtcacgca gcaggccaaa gagaacttcc tggtatccag cagcctggat
      601 atgaagctgg tgaacaacag ctttgcgacc ttctttgatt cggtgggtta caccgtttcc
      661 gccctggcgg aataccccgc ggtgaaggat gccgagaatg gtgagctgac cacctacttc
      721 ggtgaggcgc gtaagccggg tgaggtggcc accgccaacg gcgggcgcga gaagcagatt
      781 ttcgattact tctccagcat cggtaagaac aaccccacct tcggctacgt gtatatgagc
      841 gataccaagg gcggctatgt ggagtggccg ggcaccgggg attacggtga ctgggacccg
      901 cgcaagcgtg agtggtaccc cattggccgc gacgccaact tcaccctggg ccgccgcgac
      961 ggttattact gggagccgga tgatgcggtg tatgtctcgg tgctcaaggg cttcaaggac
     1021 aacgccgggc agtttgccgg ggtggtcgcg gtggatgtgt cgctcaaggc gctgacggat
     1081 atggcgcaga aggtgcgctt tggcgaaacc ggttttctga tgttggcgga gggcagcggc
     1141 accttgctgg tggatggtct gcagccggac aacaacttca agaaggtcag cgagctgccc
     1201 ggcgagcact ttgcaaccat cggcaaaacc gagaacggcc tggtcgaggt ggagatcgac
     1261 ggggtggcgt atatggccaa cgtctacacc tcgccggagc tgggctggaa gtttgtcggc
     1321 ttcaagcagg ccgatgagat ctatgcgagc gcccgtgagt tgacctggat caccctgatt
     1381 gtctgcatcg tgctggtgct ggtattcggc gcggctgggg tggttattgc caaccgtatc
     1441 gtcaacccga tcaacctggt gaaagacggg ctgcgcacca tcgctcaggg tgaaggtgac
     1501 ctgacccgcc ggctggacct gctcagcaag gatgaaaccg gtgagctggc caagtggttc
     1561 aatcagttta ttgagtcgat ccagggcatg atcaaggtga tcaaggagaa cgccatcagc
     1621 atggatgggg tttccagcga taccaacaag cgcaccacgg cgatgtccgc cacgctgcag
     1681 cgccagttga gttcggtgga gcagattgtc acggccgtga ccgagatgtc ttcggcggcc
     1741 aatgaggtgg ccaagacctg cgtgcagacc gccgatgtgt cggagcaggg gctgatggcc
     1801 acgcgcaatg gcaaagaggt gattgcccgc agtacctccg gggtcaacaa gctcggcgcc
     1861 agcatccaga actccagcaa ggtgctgcag gagctggaga aggaaacggt caatatcaac
     1921 aacatcctct cgaccattca gcagatcgcc gaacagacca acctgctggc cttgaacgct
     1981 gctatcgaag ccgcccgtgc gggcgagcag gggcgcgggt ttgcggtggt ggccgatgaa
     2041 gtgcgcaacc tggccaaacg cactcaggac tctaccgagc agatcaacag catcctcaac
     2101 ctactggtca atcgcatcaa ggaagtgacg gtaagcatgg accagagcct ggtcgagtcg
     2161 ggcaaggcta tcgtgctgtc cgatgaggtg atggccgcct tcgaaagcat tgaaggcaag
     2221 gtgcagatga tccgcgacat gaccatgcag atcgccaccg cgaccgagga acagcatctg
     2281 gtgaccgagg aaatcaacca gaacatcgtc gccatcaacg acgcggtaac gcagatctcc
     2341 gtgcaggccg ccgaggtgga aagctacgcc caggaacaaa gcggcctgag cagtgagctg
     2401 aagcaactgg tggtgcgttt ccggacggag taaggctgcg cgagttaact cccagagaaa
     2461 agccccgcaa ttttgcgggg ctttttattt accagcgatg gccatgacca tggccgtcgt
     2521 gccgacggtg tttgtggcca tgccagcgtt tatggcgcgg ccgttcctgc acgtaataca
     2581 cgggggccgg ttggtaataa accggctggc gttcgtagta cacccgttgt ggcgggtaat
     2641 aggccggtgg tggcgggtag tactcgcgcg gtggcggggc gtagacctgt acggggcggg
     2701 tttggctgac gataacgccg ccaatcaccg cgcccaccac ggctccggca atcgctgcgt
     2761 cacggttgga ggcctgcgct ggggcctggg caaacagcgc caggctggcc accaggccgg
     2821 tgataagggg gatacgcgac agcatgatcg aaactcctct gcaaaccggt ctttcccggc
     2881 atgttgtttc gacactggct gttgcgcggc attgcagggc gaaatgtaaa gcctgtgtaa
     2941 aacagggcgt gggccggaga agtaactatt tggcagtatc ggcaggctgg cggagcaaaa
     3001 aaataattca ttgcgccgct tttgcttctt tacttctgcc ctaggcctgg agccagaagc
     3061 gcagaaccct ctcaaccaag gagtcactta tgctgagtcg caaaggattt atgctcgtcg
     3121 cagtcgcctg gctgttggcc tgtcacgcgc aagccgctga tgtcacggtc aagctgggca
     3181 gcaccgagcg cgtcacccgt ttgtttgcct atcccaataa ctgcaatgtg atctgctatc
     3241 gcgattggac attggagcag accgctgagc actatttgcg ccagagcctg caacgcgatg
     3301 gttacgacaa agccacggtc accgtgcacc gcgaggagca agagcttttc gccaaattca
     3361 gcgaggtgcc tgaaggctac ggtaagccac tcgagcaact gctgaagacc ggcgagctgg
     3421 cctacagtgg cgccaaactg ctcaacaagg atggcaagtg gcaatacaac tggtcgctgt
     3481 tcctgccgct gggtatggcc ctggataacc gcaagagcgt tgagttgctg cactttccgc
     3541 cggattactc gctgacccag gcgcaggact atctagagtc gagcaccacc aaccgctggg
     3601 ctgatctgct taccgagaac ggcatcagtg cggcgcagac accggccttc cagaccatta
     3661 tcgacattgc cccaatcgcc gcgccggcca acgccggtag cgcgcttgag ggtgtctaca
     3721 gctatttcaa cgcttaccaa acacagatgg tgatgcaatt gaccgctggt gaaggcggcc
     3781 aagcgctgcc catggtggcc tttggcagtc cggtgcgcag ctgggttaat cagcagtatg
     3841 gggtgtctat cgatgtcctc ggcctggcgc agatcagccc ggctgctggg cagcaagtgg
     3901 ccgtgctcgg tgccaaccac cccagctaca tctggtatgc cgctgacccg gaaaactacg
     3961 atggcgatca gcagaaagcc gatgctgctg gcctcaaggt catggggcag gatcttagcg
     4021 ctgcctgctg gcaggccagt atgggtgagc aacccagtca ggacgcgagc ccggtgctga
     4081 gcgcctgcag cgagaaatgg caggtcacgg aaaagctgca aacctgcgag ctgttctaca
     4141 cctcggtgcg caacctcacc cccgaagctg cgcaggccaa gtgcactccg gcgagtagct
     4201 aaatgccccg atcaaggccc agtgctctgg gctttgatcg ctttcgggct taaggaatag
     4261 ccggtgtttg atttccagct gtagctgctg acacctcagg tgttacagct ggcacctggt
     4321 tttgcgcgtc tttgcggcat tgcgcctgca gggtagccca gccgtctgca ccgagctctt
     4381 ccagagtctg gatattggct tcgaagatcg tctccgggtc gggaaaggcg gctacagcgc
     4441 ggtcgatgct ggcttcgcgg aggatatgca gggtcgggta gggcgagcgg ttggtggcgt
     4501 tgcccatatc cgcggcatgg gtgtcggcaa actggaactg cggatggaag ctggccactt
     4561 gcaggatgcc gctatagccg aggtcgtcta gggcgtcgtc ggctacggcg agaaagtcgt
     4621 tgaagtcgag aaagtcggta agggccgctg ggtgaatcag caaagtagtg tcggtttcgc
     4681 ttggctcgca gttggccaag tggtgcagct catcaatcag catggcgagc agttcttccg
     4741 gctcggcgct ggcgcacacc acataacgca cttgcccttt gacctgcacg gccttggcga
     4801 acgggcagag gttcaggccg attacggcgc gatcaaccca ggcgcgggtt tcggcaatgg
     4861 cgtgggcggc gctgggggtg gcggtgctgg gcatagaaca atactcgggt tggcgtggca
     4921 tgaagtttag cgtcaatagc ggcgcgcagg ggtcgcgggg cggttgccag cgcttagccg
     4981 ctgttgcgtt gcgggttgcc ggcgcggcgt agtggattgc actggctggg gtcgatggcg
     5041 ccgatatagg ggttgccgtg gcccatcacg cagccgacct gctgattgcg ccagcgctcc
     5101 cattcgctga tcgggtattg gcggctccag gcttcgtagg tgcgccggtc gagcttggac
     5161 aggcgcagct ggtaacgctc ggccatatac aggtagatgc gtgctgccgc gccgcgcgca
     5221 tggaccggcg gcatggcagt tttcgccttg aagttgacca ccatcgggca gcttccgtat
     5281 tgcgtgggtt tgtcgctgag catgccgagg gcgtagttgg agcggtcgcc attcacttcg
     5341 ccgatgctcg gcaccaggtt gtgtaggtca gcttcggcag ccttgaagct ggcgtctgtg
     5401 cgagtgcaat tcttgcgtcc gccgttctgc cagcactgac gctgatggcc gatcacccag
     5461 gccggcacca catgctccca ttccacgcgg gcggcgcgcc gctcctgctt gcgcactttg
     5521 taaccgcagc tgccgaggtc gacccggtta ccgctatacg cgcattggca atagaaggta
     5581 ttgggccgct cggcatacag gcgccaggcg attttcttcg cggcgctaaa ggtttttggg
     5641 gcatcggcaa aggcgggcag ggcaactaac aggcaaaaca gggcaagcag acgcagcatg
     5701 gggcactcgg cagccaaaaa gctgcggagc ataccgctta aagcgctctg cgacgggcct
     5761 tttgccgctg cgctgataag cggttgccaa ccttttgcgc agtacgcggt cttttagcag
     5821 tccacctgag cttatgtggc taggtgcttg attgtgttca aagtcatgtc ttcgggtagg
     5881 aaaactggcc aatctggatt ttgttgctag cgttacccca tcacaccgca gcggacgacc
     5941 tcctctgatg agcactgtgc agcgtttgcc atggctaaaa actgcccccg cctggtcggg
     6001 cggcaatgct gtggtgatgc gcctgctggt gctgattgtg ctgttgctgg ctgtcagcgg
     6061 ctgctcaacc cggcaggacc atcagaccat tatcagcaac cgcagcgcga tcaaaccttc
     6121 cgagttcttc cagagccatg ccgaccgcat ggccacgatc ggcatgcgta acaacctcaa
     6181 cagcctctac cgtttgatgg acaagctgta cctgcgcaac ccgcgcgagt ggcgcaaaac
     6241 cggcctggct gatgtagaag ccgccaagcg tgcggtacat cgctccatag aactggatca
     6301 gccgctgccg cagctgggcg ggcgtaccga tgttgccgct ctcggctatg ccttgagcgc
     6361 cgaataccag ggcgatcgtg tgggggcctt tatttattca ctggcgagca tgctgattac
     6421 cgcccatggc gggcgcaccg agtattacct gagcgacacg ctgaacccgc agtttgtcca
     6481 taacgcggcg cgcaactttg agaaggccag ctggatgctc gctcagcgta ccgatgccag
     6541 cggcaagccc tggctgctgt ccaacgagat ttccgccgag gggcataacc tcagctatgc
     6601 cgtggagttc ggcaagatag tcgcgcgcct ggatctgctc accgatgtgc tggatgagcg
     6661 ctatcggcgc atgggcgtga gttatgccca ggggctgctg tttctgaact tcctgccggt
     6721 gcaatagggc tgtttacgtc ttgccttaat cgcggtgtcg gcccttggct gtgttcggca
     6781 cgtctgcggc cagtcgctcg agaaacatcg ccagcgccac ctcctcggct ttcaggccct
     6841 tacgcaccct cggcttgggc agtttggata acgcgcccag gctgaagtgc tccaacacgg
     6901 cagggtggat atagcatttg cggcacaccg caggggtgtt gcccagttgc ttggcgacgg
     6961 ccttgaccgt ggccaccaca tgccgtttcg cgtccgactc tggctgccac tccagctcgc
     7021 gcaacacggc caacgccatc gcggtaccgg cccaggtgcg gtaatccttg gcggtgaagt
     7081 cggccccggt aagctggtgc aggtaggcgt tgacgtcggt ggagctgatg gtgtggcgcg
     7141 ctccatcttc gtccagatac tggaagaggt tctgccccgg caattccatg cagcgcctga
     7201 ccactgtggc cagtcgccgg tccttcacgc tgacttgatg ttcgatgcca ctcttgccga
     7261 caaactggaa cttgatatcg ctgccctgga tagcaacatg gcgcgtgcgc agggtggtca
     7321 ggccgtagga tttgttgtct cgggcgtatt ggctattgcc aacgcgaatc agcgtggcat
     7381 ccagcagcat caccacggtg gccagcacct tctcgcgggt aaaacctggc gtggctattt
     7441 gcgcttgcag ttgctggcgc aatttgggca gggcattacc gaacgcttgc agacgcgcgt
     7501 atttatcggt gtcgcgcacc tcacgccatc gcgggtgata gcggtactgc ttgcgcccac
     7561 gggcgtcgcg gccggtggct tgcagatggc cgcgcgggtc ggcgcagatc cacacgtcgg
     7621 tataggcggg cggcaccgcc agtgagtcga ggcgcttgat ttcatcggcg tcggtgatgc
     7681 gctcaccctt ggcatcgaaa taattgaacg tgccgcgcac tttcttacgg tggatgccgg
     7741 gttggctgtc gtcaacgtaa tgcaggtcgc ggggcagatc agcaggcgac gcggaatcgg
     7801 gcatggcgca atgtccttgg cagccagtca ggtgggtata cctgattgac cgcagcggtt
     7861 tgccggcgtg ccaaaagctt ttttaccgag ggctcagttc aggtctgtta ttgcttgttt
     7921 gctggcgggt gttgatcagc gacagcacac cgttgtgtgc aacagcagct gcccctgctc
     7981 aatagccttc ttccaagcgc ttattgctct gtgcattagc cgcttcgatc tgctgcttca
     8041 gctgttcagt ttgctgctgc agttgcgccg cctgttcggc ttgcaggctg gtggttgccg
     8101 ctgtttcacc gaggccgcag ccgctcaggg tggttgctag cagcactaac gcaagggtga
     8161 cgcgcatggt ggctcctgag tgaagggatg aaaggcacgg cttactgcca gcgcttataa
     8221 atgctctcca aagtgccagc ctgtcttaaa cgctccagcg ccgcagcgaa ctgcttggca
     8281 cggccgctat cgcccttggc gaaggccaca tagcgcggca tttccaccag cggcttgggc
     8341 aggatatgta cgttcttctc cgcgcgttgc tcgcgcacga agtacatcag ctgcacccga
     8401 tccccgacta ttacatcgat acgcccggcc agcagcatcg acaccagttg ccgtgggttc
     8461 tgtgcgctgg tgtcgcgcga cagctcggcc ttgtcgaagt ccgactggta ggcatagccg
     8521 cgtacctggc caatgatata gggcgacagg tcgtcgagtt cggcccagtc gttgagggtg
     8581 attttgcgcg aggtcataaa caccgtggag ccggtgcgga tcggcccgac gagctcgtat
     8641 tgctgcatgc gctcaggggt accggcgaac tgaaaggcca tggccacttc gctgcggtcg
     8701 atcatgcgtt tgacccgttc ccagggatac aggcgcaggc tgtaggggat cttggcttcc
     8761 tgcagcaccg cctccaccag ctcgacatcc agcccgcgcg gctggtcgtc ttcgatggtg
     8821 acaaagctgt agggcgcgaa ctgctcatcg cccaccacct tccacggctc ggcctgcaca
     8881 gcccagggca gtagcagcat caggcacagc gcagcaatac gcatacgatc cctccaggcg
     8941 agcgcctcga acgagcagtt tgctctgtat tgatgctagc caatgaaaaa agccccggca
     9001 tgcaggccag ggctcttttt tgttgccggt ggtgcgctca gactttgcgc acgaactcgg
     9061 atttcagctt catcgcgccg atgccgtcga tcttgcagtc gatgtcgtgg tcgccgtcgc
     9121 acaggcggat gcccttgacc ttggtgccga ccttgaccac cagggatgaa cctttgacct
     9181 tgagatcctt gatcacggtg acggtgtcgc cgtcctgcag catattgcct accgaatcct
     9241 tgatcacctt ggcgtcatct gccgcgtcgc tcgcgccagt ggccgaccat tcgtgggcgc
     9301 attccgggca gaccagctga gcgccgtctt cgtaggtgta ttcggagtta cattttgggc
     9361 agggcggcaa cgtgctcacg gcgggtcctt gatggggtga taagaaatgg ccgcatattg
     9421 tatagggttt tatcgcgctg cgccgggcgc tggcggttgt ggccagttca gcgtgccacg
     9481 gtcattgaaa cgccaggtac cgaacaggcc gttggccagt ttgccgcgca attcataggg
     9541 taggccctgg ccgtcctgat agccggccat gccccaggct tgacgcagcg cggcgtaggc
     9601 ggaaaggctg accggaatac gcaggatgct ttcgccgtaa cggggtactt gcccgcgttg
     9661 gtcactgacg ccactgagca gcggttgctg gttgacctgc aattccaggt ctacgccgtc
     9721 gaaatcgatg ggctggtcat tgggattctg tacccgcagt ttgatcgcaa agcgcgcttc
     9781 caggccttcg ccgggcagcg gttcgatacc gaccagctcg atgcgtaatg ggtcgcgcgg
     9841 ggcgagggtg ctgcacgcgg ttagggtaag cagcaggctg acaagcaggg cgcggacgag
     9901 tgtgcggtga agggcgttgg gcatgctggg tttccactgc tgaataatta cagagtagaa
     9961 gaccccgaga gcggggcgat tacgccgcag ctgggcaaat tagcgcgaga aaggttgttg
    10021 cggtgaatgc ccgccagcct gcagtcgcag gtgctggcgg gtttgtagcg acttatcaga
    10081 agtgcgcttt taccagcaga ctggcggtgt tctgatcggt gccctggagg atttcgtcac
    10141 cgaggaagct gttgtcgtcg atgccgtatt tgtccttcca atagtcgtat tcgatgccga
    10201 catacagctg cttctcgccc cagttcatgg ccttgcccag gtcgtacttg atctgtgggt
    10261 tgaagtgcag gttggcgtgg tagctcttgt cgttgtccac cacccagtcc ataaagccgt
    10321 cgatcacgat gtcggatttg cccaccggga tggtgtagct ccacaccggg gtgatctgcc
    10381 agacattatc gccgtcgcgc ttgccatccg ttgtccggtt gtagaagttc agcgagaagt
    10441 agtcgaagcc ggggatcgcc agatcaaagc ccgggccgat caggtaggaa tcgacatcgt
    10501 cctcaccgaa ttcataggtg ctggccagca gcacgtcctt gattgggccg aattccagct
    10561 tggtattgaa cagcttgccg aacgacaggc gcggtgagaa ctcgccgtag aaggtgtgac
    10621 cgtcgcctgc gccattggtg gcgtcggtgt tgtacttgat agtgtcgacg aagaagaaca
    10681 ggtcgccgat agcccagcca ctggcgtgtt cgaaggtgat ggtctgctgg atttccgggt
    10741 cgatcttgta atcctggcca tacaggtagg tcaggctgtt gttctgccag tgcagcaggt
    10801 cacccgctac ggcctggccg cccgccagca gcccgcccgc caggaccaga gaagaaagag
    10861 tacggttcat cgagtggtgc tccccaaagt gctgaattat ggttttaaac ctgtctaagt
    10921 ggtcaggctg tcggagcttg agcaagaaca aggccaagtg ctgaggttgt ttactgcacg
    10981 cccacgcaga gcgacgcggg cgcgcactct agtggttttc aggggaatgt aaatgcgctg
    11041 catgcactga taactgaccg ggtggacagt ttcggcttta agttggtgcg cctgtgcttg
    11101 agagtaggtt ggccgaaagc agtcgaggtg cgcctagttt tccagtgttt ccacaccctt
    11161 cagcaccacc tgctccagtg cgttgcctgg atcctgttgt gtgccggcca gttggccgag
    11221 tagctgccat tgctggtcca gctcgctgtt gatcgccagc atgcgctcga tcatcttgtg
    11281 cgcggtggcg ttcacggcgc tgtcctgggt gttgagcagt tcaaacgaca tggcggtgat
    11341 ggcggcagtc aggtgggtga gggtgcgcgt ggacagactg agcaactcgg tcaattgcgc
    11401 ttccttggca gtcatggtcg tctccttgtg aatcctgttc tgagcttagc gcgacatcgt
    11461 gtcacccggc tatctgctcc atcgcctggg gagtaccgcc gggcttgtgt gcagtttctt
    11521 tctcctcttt attaataggt ctatggccgg ttgcactgat cgcatacgca ctccgtcggc
    11581 caatgcgcgc gctggtatct ggtgtgcttt gctcagttag gacgtgaata gaggaagcgg
    11641 tacaacctat tggctgtcgt ggttgtacct gtcgtcggaa gcgctgcggt tttttaaaac
    11701 gagcgttttg cttacttagc gtccgatttt tactggaact gcggcagaac tgggcttgcg
    11761 ccagaacgca gctggattac tggcagctga aggccgtaaa aaactctgat tttgtttggt
    11821 tgcacctagt tgcaccctgc gccttgtggg gtttaatctc tcgccatatt ttttgtgacc
    11881 cgccacccat ggcggccacc tctcggggcc gtcgcggggc aacgtccaaa attgctcccg
    11941 gcgatttttt gccgtgctcc agcccataca ggacaaacca atggccacca ccacccttgg
    12001 cgtaaagctc gatgatgcaa cccgtgatcg cctcaagcag gctgcgcagt cgattgaccg
    12061 cacgccgcat tggctgatca agcaggcgat ctttaattat ctcgagcaga tcgagggtgg
    12121 tttgaccccg gccgagcatt cggggctggc ggcagtcgcc ggtgaagagt cggtggattc
    12181 cctgaccgag cagggcctgc aggtgttcct cgattttgct gaaagcatcc tcccgcaatc
    12241 ggtactgcgc gcggcgatta ctggcgctta tcgccgtccg gaaaccgaag cggtgccgat
    12301 gctgctggag caggcgcgcc tgcccaagga catggccgag gcgagcaaca cgctggccat
    12361 gaagattgcc gagaagctgc gtaaccagaa gaacgccggt ggccgtcagg gcctggtgca
    12421 gggtttgctg caggagttct cgctgtcctc gcaggaaggt gtggcgctga tgtgcctggc
    12481 cgaagcgctg ctgcgtattc cggacaaggc cactcgtgac gcgctgatcc gcgacaagat
    12541 cagcaacggc aactggagcc agcacctcgg caacagcccg tcgatgttcg tcaacgcggc
    12601 cagctggggc ttgctgatca ccggcaagct ggtggctacg cacaacgaag ctggcctggt
    12661 gacttcgctt aaccgcatca tcggcaaggg cggcgagccg ctgatccgca agggcgtgga
    12721 catgtccatg cgcctgatgg gcgagcagtt tgtgaccggc gaaaccatcg ccgaagctct
    12781 ggccaacgcc accaatatgg aaagcaaagg cttccgttat tcctacgaca tgctcggtga
    12841 agccgcgctg accgacgagg acgccaagcg ttacctggcg tcctacgagc aggccattca
    12901 tgccatcggc aaggcctctc acggccgtgg catctacgaa ggcccgggta tttcgatcaa
    12961 gctctcggcg ctgcacccac gctacagccg cgcgcagtac gagcgcgtga tggacgagct
    13021 gtacccgatc ctgctgggcc tgaccaagct ggccaagcac tacgacatcg gcctgaacat
    13081 cgatgccgaa gaagccgacc gcctggaaat ctccctcgat ctgcttgagc gcctgtgctt
    13141 cgacccggcg ctgaccggct ggaacggtat cggatttgtc atccaggctt accagaagcg
    13201 ctgcccgtat gtgatcgact acgtgatcga cctggccaag cgcagccgtc accgcctgat
    13261 gatccgcctg gtaaaaggcg cctactggga cagcgagatc aagcgcgccc aggtcgaagg
    13321 cctggaaggc tacccggtct atacccgcaa gccatacacc gatatttcct acatcgcctg
    13381 cgcgcgcaag ctgctggccg tgccggaagc catctacccg caattcgcca cccacaacgc
    13441 ccactcgctg tcggccatct accagctggc cgggcaaaac tactacccag gtcagtacga
    13501 gttccagtgc ctgcacggca tgggcgagcc gctgtacgag caggtggtgg gcaaggttgc
    13561 tgatggcaag ctgaaccgtc cgtgccgcat ctacgcaccg gtaggcagcc atgaaaccct
    13621 gttggcttat ctggtgcgcc gcctgctgga aaacggcgcg aacacctcgt tcgtcaaccg
    13681 catcgctgac accagcatct cgatcaagga actggtgctc gacccggtgc tgcaggtcga
    13741 gcagatggcc acgcaggaag gcggcctcgg cctgccgcac ccgcgtattg ctctgccgcg
    13801 tgcgctctat ggcgacgccc gattgaactc ggaaggcatc gatctggcca acgaacaccg
    13861 cctgggttcg ctgtcctcgg cactgctgag cagcaccaac accgtctata aagccatgcc
    13921 gctgctcggt tgcgaaacgc ctgagctgag cgacgcacag ccagtgcgca acccggcgga
    13981 ccaccgcgac atcgtcggcc atgtgcatga agccagcgag caggatgtac gcaacgccgt
    14041 gctttgcgcc gtgtccagcg ggcagatctg gcagtcgacc ctgccggccg agcgcgccgc
    14101 cgtgcttgac cgcgccgccg accagatgga agcggaaatg cagcagctga tgggcctgct
    14161 ggtgcgtgaa tccggcaaga ccttcgccaa cgccatcgcc gaagtacgcg aggcggtgga
    14221 cttcctgcgc tactacgcgg cgcaggcacg taatcacttc agcaacgaca gccatcgccc
    14281 gctgggccct gtggtgtgca tcagcccgtg gaacttcccg ctggcaatct tcagcggtca
    14341 ggttgccgcc gcgctggctg ccggtaacac cgtgctggcc aagccggccg aacaaactcc
    14401 gctgatcgcc gcgcaagccg tgcgcattct gctggatgcc ggtgtaccga gcggtgccgt
    14461 gcaactgctg ccgggccgtg gcgaaaccgt cggcgccggc ctgatcggca acgagcgagt
    14521 acgtggcgtg atgtttaccg gctccaccga agtggccggg attatccagc gcaacctcgc
    14581 tggtcgcctg gatgcccagg gccgcaccat tccgctgatc gccgaaaccg gcggcctcaa
    14641 cagcatgatc gtcgactcct cggcgctcac cgagcaggtt gtggtcgatg tggtggcctc
    14701 ggccttcgac agcgccggcc agcgctgctc ggccctgcgc gtactgtgtg tgcaggaaga
    14761 cgtggcggat cgagtgatca acatgctcaa gggcgccatg gccgaataca gcctgggcaa
    14821 cccggagcgc ctgagcaccg atatcggtcc ggtgatcgat gctgaagcca aggacaatat
    14881 cgaccagcac atcgagaaga tgcgcgagaa gggccgcaag gtattccaga ccgcccgcgc
    14941 cgtgggtgac gacatcaagc gcggcacctt tgtgctgccg acgctgatcg agctggacag
    15001 cttcgacgag atgcagcgcg agatcttcgg cccggtactg cacgtagtgc gctaccagcg
    15061 tgctgacctg ggcaagttgc tgcagcagat caacgacagc ggctatggcc tgaccctcgg
    15121 tgtgcacacg cgcatcgacg aaaccatcgc ccaggtggtc aacaccgcca aggtcggcaa
    15181 cctgtatgtg aaccgcaaca tggttggcgc tgtggtcggc gtgcagccgt tcggcggcga
    15241 aggcttgtcc ggtactggcc cgaaagccgg tggcccgctg tatatgtacc gcctgttggc
    15301 gacgcgcccg caggacgcag tggccaagca actgcagcag gaaaatggcg aaatcacccg
    15361 tcctgccgag cagggcaagg tgatccaggc cttgcaggat tgggcggcca agcatgagcc
    15421 gagcctgact gcccttagcg cgcagtatgt tgagctggcg cagagcggca ccgtgcagat
    15481 gctcaacggc ccaaccggtg agcgcaacag ctacagcctg ctgccacgtg agcatgtgct
    15541 gtgtctggct gatgagcgcg gtgacctact ggtgcaactg gcggcggccc tggccgtggg
    15601 ttgccaggtg ctgtggcagg aaggcgaact gacccgcgag ctgtttgccg ctctgcctaa
    15661 ggccgtgcaa caacgcatca cagtgcttag ccagtggagc gagagcgaaa cggtgttcga
    15721 cgccatcatt catcacggtg actccgatca gctgcgcgaa gtctgccaac tggcggctca
    15781 gcgccctggc gcaatcatcg gtgtgcatgg tctgcacaaa ggcgagacgg atatcccgct
    15841 ggagcgcctg ctgatcgaac acgccctgag catcaacacc gcagcagctg gtggcaacgc
    15901 cagcctgatg accatcggct aacaggttct acgagcatcc tgccccactg gtttaccagg
    15961 tggggcagtt ggcaacaccg gcttaccgcc accccggaga cctgttttag gtgtctgatg
    16021 ctgccctgca gcgctcaacg cacaaccggc aacggtgggt tacccgcccg gtgtgttggg
    16081 tttcttccct cgggcatttc gatgcatgac ccctgcctac ccggcgggtg tcgcgacgat
    16141 gtaacacgcg ccgtcgcgca tcgaaggcca ttccttctct gactccattc tgcgcgtcgc
    16201 ctgccaaggc tctgtgtggc tgttttttgc gagcctggaa aatacctgtt cgcctcattg
    16261 acgcgcctgt gccagtatac ccctatgggt atatttaatt gaggcgaggt agatgatgcg
    16321 attgttaaca gcggtgttaa tcgttggcct gagcgtgagt gcgcaggctg acaatctgca
    16381 agcgttgact gctgaaggca tggcgctgat tccgccgttt cagcagcagc tgatgggcac
    16441 ggtcaaagcg gcaatgagta gcggtggcgc ggcgcacgcg gtagaggctt gtcagctgtc
    16501 tgccccgcaa attgccagcg agcacagcca aaagccatgg acagtggggc gcacggcgct
    16561 ccgggtgcgc aacccggata acgcccccga tgcctgggag cgtgaagtgc tggcgcagtt
    16621 tgcccggcgt gcggcggccg gtgagccgct ggagcagatg agccaaagcg gcgtagtggc
    16681 gggcgagtta cgggtgatga aagccattgg cgccgctgaa ccgtgcctgg cgtgtcacgg
    16741 taagcagatt gagccagcgt tggcgcagtt gattgaggta cgctacccgc aagaccaggc
    16801 ccgtggtttt gctcagggtg agctgcgcgg tgccttcaca ttacggcgac ctgtagattg
    16861 aacgaatgaa tgatcaacgt ttgcaacacc gcgatgccct gctcaagcgc ctggcccgcg
    16921 tcgaggggca ggtgcgcggc attcaggcca tgatcaagcg cggcgacgag tgcgagtccg
    16981 tcgcgcagca gttcagcgcg gcgcgcaagg cgctcgataa ggcctatcag gaaatgctta
    17041 tgtgcttggc ggaagatgct atccgcagcc ctgagcaaag ccacgaagcc accctggcgc
    17101 aactcaaaac catcttcacc aagtacacct gattcgcgcg cctcaccgcg cgcgcccctc
    17161 tgttgccaat cgtttcggcg cgcggccgtg cttctgtcgc agggccttgc cgtgcgcgtc
    17221 attcaggccc gcagtggctt ctgcgtgcgc atggtgcggc aatacacccc atacccccca
    17281 gggtttaaag gcatacactc gtgtctgcta gacgagcctt gcgtctctcc ggctcgttca
    17341 gtcagttcca cacacaggat ctactcagat ggaattagac ccgcttttcc tctcgcgact
    17401 gcaatttgct tttgtggtct gctttcacgc cattttcccg gtgttcacca tcggtttggc
    17461 gtcctttatc gcctttctgg agggcatgtc cttccgcacc gctgatccgg tctgggcgcg
    17521 cctgtcgaag ttctggactc aagtgtttgc cgtggtcttc ggcatgggtg tggtgtcggg
    17581 catcgtcatg gcctttcagt tcggcaccaa ctggagcaac ttctcctacg cagcgtcgaa
    17641 cttcctcggt ccggtgctga gctatgaagt gattacggcg ttcttccttg aggccgcgtt
    17701 cctcggcgtg ctgctgttcg gccgcgacaa ggtgccgccg ggcgtgcacc tgtttgccgc
    17761 gtgcatggtg gcgctgggta cttttatctc ctcgttctgg attctctcag ccaacagctg
    17821 gatgcaaacg ccagttggca ctgagctgcg tgacggcatg atccatgtcg tgtcctggtc
    17881 gcaggcgctg ttcagcccct cgtttatcta ccgttttatg cacatggcgc tggcctcgtt
    17941 cctcacaggg gcctttgtgg tggcgggtgt cagcgcctgg tacctgctgc gcaagcgtga
    18001 agtggaagtg aaccgcaaag ccttgtcggt gagcttgtgg atgatcctgc ttatcgcccc
    18061 cactcaagcg gtagtgggcg attaccatgg ccttaacacc ctgcagcatc agccgaccaa
    18121 agtggcagcg atggagggta actgggagcg tgcttctggc gtgccacttc tgctgtttgc
    18181 ctggccggat cagcaggcgc agaccaacca tttcgagatt gccattccca agctggccag
    18241 ctttattttg acccatgact gggacggcga gcttccgggt atctccgaag tgccctacga
    18301 agagcagccg ccggtttgga tcgtgttctg gtcgttccgg gtaatggtag gcattggcct
    18361 gctgatgatt gccttcggtt tgaccgggct gctgctgcgt cgtggtggtc gttactggca
    18421 agcaccctgg ttcctcaatg gcatgcgctg gatgagtttc acacccttct tggcagtgct
    18481 cgctggttgg gtcgttaccg aaacgggccg cgcgccctgg ctgatttacg gggtgatgac
    18541 acatgcccag ggcctaacgc catcgctcac cggaggcatg gcgctgttca ccctgattgg
    18601 ctacatcgtg gtctatgcga tggtcttcag tgccggcctg tactacctgt tccgtgtgct
    18661 gcaagcgggc ctggaagaag acctgagcgc agaagacacc catgaagtgg agcgggcctc
    18721 gcgtccgttg tcggcaaccc atgtacgtct ggaacagggg ggacactgaa atggtgcctt
    18781 ttgacctcac aatgatttgg gcggtaatca tcgccttcgg catcatcatg tacgtgctga
    18841 tggacggttt tgacttgggc gtggggattc tcttcccctt cgccccggac gagcaggccc
    18901 gcgatgtaat gatgaactcg gtagctccgg tctgggacgg taacgaaacc tggctggtgt
    18961 tgggcggcgc gggcctgctg gcggcttttc cgctggtgta ctcggtgttg ctgccggcca
    19021 tgtatatcgg ggtgtttttg ctgctggcgg gtttgatctt ccgtggcgtg gcctttgagt
    19081 ttcgcttcaa ggccaacacc tcgcgctacc tgtggaactg ggcctttgcc ggcggctcga
    19141 tcattgcttc gttcgcccaa ggcgcagtgg tcggggccta cattcagggc tttaacaccg
    19201 aagggctggt gtatgtaggc ggtgcgctgg attggctgac gccgttcact gtgctcaccg
    19261 gtatcggcct gctggccggt tacgccttgc tcggctgcac ctggttgatc atgaaatccg
    19321 aaggctacat tcaggactgg gcctacagcc tggccccgaa actgctggcc gcggtgttgc
    19381 tggtgtttgt cgccatcagc atctggacgc cctgggttga tccatcggcc tatgcacgct
    19441 ggttctcggc catcggcctg atctggatct tcccggtgat ggcagcattg tgcgccctgc
    19501 agattttccg cagcctgcgc aaacgccgtg aggccatgcc ctttatcgcc accatgggtt
    19561 tgttcatctt cacctacctg gggctgttgg tgaccaagtg gccgtacatc attccgccta
    19621 accacaccat ctgggacgcc gcctcagccc ctgaatcgca gttgttcctg ctgttgggcc
    19681 tgttgttcgt catcccgatt gtgctgacct ataccgcctg gacctactgg gtgttccgcg
    19741 gcaaggtgaa agcaggtgtc ggctatcact gataaaaccc gcaagcagcg cagccgccac
    19801 tggctgaccc gtcagcagca ggccgtacgc ccgttgctgc tgggcgcggt agcggccggc
    19861 gtgctgggcg gtgtggcgat tgcggcgcaa atgggcctga tggcctggct ggtgcacgcc
    19921 ttgctgatcg acgcggcccc ggcgctgagc ctttggccgg tgatgtcggc tctgctgttg
    19981 gcggtgctgt tgcgcgtgac tgcggcggcc gtgcaggagc gactgggcca actggccagc
    20041 gccaaggttc gccaacaggt gcgggtggag ttgagcgcct tgtgggcccg gctcgggccg
    20101 gtgcgtttgg cggagctgcc cagtgccagc ctgaccaacc aatgggtcga gcaagtcgac
    20161 gccctcgacg gctattacgc ccgttacctg ccgcagcaat ggctggccgc actggtgccg
    20221 ttgatgttgg ttgcgctggt gttctggctg gattggctgg cggcgctgtt tctcttgctg
    20281 gccgcaccgc ttattccgtt gtttatggcc ctggtcggca tgcatgccga acgcatcagc
    20341 cagcgccatg tgctgcaggc cgggcgcatg gccgggcagt ttctcgaccg cgtgcgcaac
    20401 ctcaccagcc tgcagttgtt tggccaggtc gggcgctcta tcacacgtat cgaacaggcc
    20461 acgcaggctt atcgcacggt gaccatggcc acgctaaagg tggcgttttt gtcctcggcg
    20521 gtgctggagt tttttgcctc ggtggcgatt gccgtggtgg ccatgtacat cggctttggc
    20581 ctgttgggct atatcgacta cggcccatcg cctgaactga gtttgtgcag cgggctgttt
    20641 attctgctgc tcgcaccgga gttttttcag ccgctgcgca gcctcgcgca gtattaccac
    20701 gacagggcgg cggcgctggc agccgccgat gcgctggccg aactggaggc gctggacagc
    20761 ctgcctgctg cgccggaaga cgtgccgaat cacgcggcag agcccggcat tcaactgcgc
    20821 gatgtgcacc tgagctacgt cggtcgcggc gagattctgc gtggggtgaa cctgcacatt
    20881 aatgccggcg aggtgctggc gctggttggg ccatcgggtt ccggcaagtc gagcctgctg
    20941 catggcctgg ccggttttat ccagccgcag gcggggacga tctggctgtt cggccagccg
    21001 cccggccagc cgcccggcca gcaggccgtg gcctggatgg gccagcggcc atttctgatc
    21061 aaaggcagtt gggccgaaaa cctgcgcctg atcgcgcccg aggcggatgc gtccgccatg
    21121 caacaggcgc tggcgtcggt ggggttgagc gcgctgctgg ccgctcaacc gcaaggtctc
    21181 gacagcccgc tgggcgaggg cgggcgcggg ctgtccggtg ggcaggcgca acggctggcc
    21241 ttggcgcggg tatggttgag cagcgcgcag ctggtgctgc tggacgagcc caccgccagc
    21301 ctcgatgaac agagcgaagc ggaggtgatc aaggctctgc aggccttggc cagcagcggg
    21361 cgcacgctga tcatcgccac ccatcatccg gccctgatgc acatggcaaa ccgcgtggtg
    21421 cgcctgcacg acgggaggtt gctcgatgca tgagctgagc ccctggctgc gtgacatgcg
    21481 ctactggcgc gggcgtttgt gcgtcggtgc actgctgatg ctgctgacgg tgctcagcgc
    21541 cattggcctg ctggcgttat ccggctggtt tatcaccgcc acgggcgtca ccgcgttgct
    21601 ctgggcggcg ggaaaccggg tgtttttcga tatttatgtg ccgggcagcg gcatccgctt
    21661 ttttgccctg gcacgtacgg tggcgcgcta caccgagcgg gtgttcaacc acaacacggt
    21721 gctcggtttg ctcgccgatt tgcgcgtgcg ccacttcagc gccctggcgc aactcgacgg
    21781 cgcgacgctg ggccggctgc gcgcggcgca gtggctcaac cgtctgaccg cagacattga
    21841 tgccctcgac acactctacc tgcgcctgct ggctccgccc gctgtggcct tgctggcgac
    21901 gctgctggtg tgtgggctgg tggcgctgtt ccacccgtgg ctggcgttgc tgttgttgtt
    21961 gctgttgctg gcactgttgc tgctggtgac ggtgggcatg gcccgcgccg gcctgcacct
    22021 gagtgcccag cgcgtggtgc aattggatag cttgcgcgtg cagtgcatcg agcagttgca
    22081 gggcttggcc gagttgactg ttgcaggcag cctcggcctg catcaacaag ccttgctggc
    22141 gcgcagcgag caactgatca acgaccaact ggcgttgcag gggcgggtag tgctggggca
    22201 ggccttgagt ctgcttggcg tgttgggtgc gagtttgctg ggtctgtttg ccggggtgat
    22261 cgcctatcag caagggctgc ttagcgggcc actgatggtg atgattgccc tggcgctgat
    22321 gggccttaat gaatcttttt ccgccttgcc ggcggcgttc gcgcaatggg gggctacctg
    22381 cgcggcggcg gcgcggctca accaacaagt ggccatgagc ggacgtttgc ctcacgccgc
    22441 cagcccggtt gccgtaccgg ctaacctgac cttgagctgg caggatgtcg ccgtaatcca
    22501 tgccggtttg agcggcgtgc agttgcagct tgccgctgga gaacgcctgg cgctggtggg
    22561 gccttccggg tgcggaaagt ccaccctggc ggcgctggcc gcacgtttgc tcgacccgga
    22621 tgctggtgcg gtgtgtgtgg caggcgtgcc tttgacggca ttggagctgg acgcttggcg
    22681 cgggcaaatc gcctacctga cccaggaaac cgaattgctg cacggcagca tcgccgacaa
    22741 cctgttgctc ggttgcccgg atgccagcga tgcgcagctc tggtgggcgc tggacatggt
    22801 cgatctggct gacaccgtgg aaagcaccgc caatggcctg aacacttggg tcggtgaaac
    22861 cggaaaacag ctttccggtg gcgaaggccg ccgcttggcg ctggcgcggg tgctgctgcg
    22921 cgatgcaccg ctggtgattc tcgatgagcc cttctccggg ctggatgagg ccacccgtga
    22981 acgcatcaaa ccgcgcctca atgcttggtt gcagggccgc agcgcactgc tgttgggcca
    23041 cagtgaagcg gcgttgccgc aggccgatcg gctgctgagc tggcagcaac tgccgcgcgt
    23101 gccgcgatga tgcttctgta ggttggcgtt gagcgcagcg aagcccaacg ggttgtagtg
    23161 tgatttgttg ggcttcgtcg ctgcgcgcct caacccaacc tacgggggcg tggggctgtt
    23221 aagggtttaa tcaaacgcgg gcaattgcgg ggtttgcacc ggtttgccga cggcgtgcca
    23281 cgcctcatag ccgccggtaa tcgacagcac gttgccatag cccatgtcct gcaatgtgct
    23341 ggccgcgagg gccgagcggc cgctgttttt gcaatacagc accaggttga ggtcgcgccg
    23401 cgtcagggcg gggtcattgc tgagtttgaa ctccagcagg ccgcggggaa tgttgaccgc
    23461 ccccggaatg tgcccggcct gatattcatc cgcttcgcgt acatcaatca gcacatcggc
    23521 tgtgcggatg gcggcatcgg cggcgtgtag atcgacttct tcgatgcgtg tcttggcttg
    23581 gctgactaga tcgtgggcgc ttttcatcag ggcatcctgt tggcaatggg tgagggctgc
    23641 gtggtctgtg gggcgagggt tttcagcgcg tcgaactggc tgataaacac ctgcccggca
    23701 aaggtgtcga gaaagctgga gcgacgcagt tggtccatca ccgggccttt gacttccgac
    23761 aggtgcaact gcacgccagc ggcctgcagg cgctcgacga tggcttccag cgaatccagc
    23821 gcgctggcgt cgatcaggtt gacgcccgaa cacatcaaca ccagatggcg cacttcgggc
    23881 cggctggcga tcagctcgcc gatgcggtct tcgaggtagc gcgcgttagg gaaatacagg
    23941 ctttcatcga cgcgcagcga gagcacgctg ggcgactcga tcaccgcgaa acgctcgaca
    24001 ttgcggaagt gttcgctgcc cggcacctgg cccactacgg cgatatgcgg ctggctggtg
    24061 cgccagagaa acagcagcag cgacaggccg acaccgagca gaatgccagc ttccacccca
    24121 atcaacagca cgcccagcag ggtcgccacc tgcgcggccc cgtcctggcg tgagtagcgc
    24181 cacgtgcggc gcagcgagcc gagatcgacc aggctcaaga cggcgacaat gatggtcgcg
    24241 gccaatacgg catgcggcag gttgtgcagc agcggcgtta gcagcaatac gctgagggca
    24301 atgccggctg cggtgaatac accggccatg ggggtttgcg cgccagcatc gtagttgacc
    24361 accgagcggg cgaaaccacc ggtcaccgga aagccgccgc tgaaggccga ggccaggttg
    24421 gccgaaccga ggccgagcaa ctcgttattg ggttggatgc gctggcggcg tttggccgcg
    24481 agggtctgac cgaccgacac cgattcgaca aagcccacca ggctgatcag cagcgcggca
    24541 ggcagcagtt gcagggccag gtcgaggttc agggtgggca gggtcaggcc cggtaatcct
    24601 tgcggtatgg caccgaccac cttcacgccg gcgtgatcga gttgcagcgc gctcaccgcc
    24661 acaatcgcgg caataatcgc cagcaccggg ccagctttgg tcaagttgcc ggccgcagca
    24721 gcaggtatgc ccaagcgttg cagtgcgccc ttcaaatgcg cccgcgccca ccacagccag
    24781 gccaggctca gcaggccgat cagcagagtc ggctcgtgca cggctgacag gcccagccag
    24841 agctgcgtga gcaggtgcag caggttttcg cccgaggccg aaatgcccag cagatgcttg
    24901 agctgaccga tggcaatcag aatgcccgag gcactgataa aaccggacac caccggatgg
    24961 ctgaggaaat tggcgagaaa gcccaaacgc agcaccgcca tggcgctgag cagcagcccg
    25021 gaaagcagag ccagcaacat ggcagcgccg atgtattcgg cgctgcctgc cgggaacagc
    25081 ggttgcaacg cggcggcggt catcagcgac accaccgcca ccggccccac cgctagggtg
    25141 cggctggaac caaacaaggc ataggccagc agcggcagga tgctcgcgta cagccccatc
    25201 accggcggta gcccggcgag catggcgtag gccaggctct gcgggatcag catcagcgtg
    25261 acgatcagtg cggccaggcc atcctggctg gcgttgcggc ggctgtagtg tcgcccccag
    25321 tcgaggctgg gcagccagcg gctcaactgc atggcgtgga ttcctgatcg gtacagcagg
    25381 ttgaagccat ggggccgttc ctcagagcac gtcgaggggg attttcaggt agcgagtgcc
    25441 gttgtcttcg gcagggggca gttgcccggc gcgcatgttc acctgcaccg acggcagaat
    25501 cagcattggc atgtctaggg tagagtcgcg ggcgttgcgc atggcgacaa aatcggcctc
    25561 gctgatgcct tcatgcacat gcacgttcag cgcgcgttgc tccgccaccg tggtttggta
    25621 gcggaagtca tcgcgccccg gtgccttgta gtcgtggcac ataaacaggc gggtgtcgtc
    25681 gggcagggca aacagcttgc gaatcgactg aaacagcgtg cgcgcgtcgc cgccggggaa
    25741 gtcgcatcgc gcagtgccgt agtccggcat aaacagcgtg tcgccgacaa acccggcatc
    25801 gccaattaca taggtcatgc acgccggggt gtggccgggg gtgtggatgg cgcgggcctg
    25861 aatgctgccg attgcaaacg tatcgccttc cttgaacagg tgatcgaact gacggccatc
    25921 ggtggcgaac tcgctaccgg cgttgaacaa cttgccgaag gtgttttgca cggtggtgat
    25981 gtgctcgccg atggccaact ggccgcccaa ttctcgtttt aaatagggcg cagctgagag
    26041 gtgatcagcg tgcacgtggg tttccagcag ccaatccact tgcaggttgt gcgccttgac
    26101 gtaatcgatc agttggtcgg cactgctgtg cgaggtacgc ccggaggcgg ggtcgtaatc
    26161 cagtaccgag tcgatgatgg cgcagcgctg ggtgctgggg tcgctcacca catagctgta
    26221 ggtgaaggtg gcgggatcaa agagtgcttc aacatggggc tgcatggcga tcgtccttcc
    26281 catataccct gaggggtatt taattaaggc taagcttaac tatttttcag ttctatatat
    26341 agatttaaaa ggaatatgag gcttggccct tgttcagtca tgccatatgc cgctgtaagc
    26401 cgctgaaaag ccgacggttc gcgacaaaac gacgactcaa tttaaaggcg cagacagcct
    26461 gctagccggt aaagaggtgt tttccacgcc acgttatatc tccaacctcg gcgcgcagta
    26521 ccaaccgcat caggattggc agttcggcct gcaggcccgt gctcagggtg attactacat
    26581 cgacgagttg aatgcgcagg gtaagtacgg cggctttgcc gtgctggacg ccaacgtgcg
    26641 ctacaacctg tcggccacca ccagcctgga tctacaaatg aagaacgttc tggatgaaga
    26701 gcacgaatac gtctggtatg acaacttctt ctggggtggt gacgaccagc cgatgttctc
    26761 tgctgcacca gggcgttcgg cctacctgtc ggtgaatatg aagctgtaag gctggcgcga
    26821 cggcttaacc gccacaggta aatcgtacaa aacacaatgc gcccagttgg gcgcattggt
    26881 taacgagcgc aggcattgca cctgcgcagc gttgcggttt acagcaggtc gaagcggtcg
    26941 gcgttcatca ccttgctcca ggccgcgacg aagtcatgca caaacttctc gcggctgtcg
    27001 tcctgggcgt agacctcggc gtaggcgcgc aggatcgagt tggaaccgaa caccagatcg
    27061 acacgggtgg ccgtccactt ggtggcaccg ctcttacgct cgacgatggc gtagctgtta
    27121 cgcccagtcg gcttccagtt gtaggccatg tcggtcaggt tgacgaagaa gtcgttgctc
    27181 aggacaccga cccgatccgt gaacacgcca tgctggctgt cgccatggtt ggtgcccagt
    27241 acgcgcatgc cgccgatcag tgcagtcatt tccggtgccg tgaggcccat caattgtgcg
    27301 cggtcgagga gcatttcctc aggctgaacc acgtagtgct gcttctgcca gttgcggaag
    27361 ccatcgtgca gcggctccag cacttcgaac gattcaacat cggtctgcgc ctgactggcg
    27421 tcgccacgcc cggcagcaaa cggcaccttg acggttacgc cggctgcttt ggcggcttct
    27481 tcgatggcca ggttgccacc cagcacgatc acatcagcga cgcttgcacc ggtttcgctg
    27541 gcgatttttt cgtataccgc cagcactttg gccagacggg ccggctcgtt gccgtcccag
    27601 tccttctgcg gagccagacg aatgcgcgcg ccattggctc caccgcgttt gtccgagtga
    27661 cggaaggtgc gtgcactgtc ccatgcggtg ctgacgcgct cgctgatgga cagcccgctg
    27721 gcggcaattt tcgccgccac ggcagctaca ttgtaatcgc tgcgaccggc tggcacgggg
    27781 tcttgccaga taaggtcttc agccggtaca tccgggccga cataacgggc tttagggccc
    27841 atatcacggt gcgtcagttt gaaccaggcg cgggcaaaca cgtccgagaa ataggcctga
    27901 tctttgtaga agcgctcgga gattttgcgg tactccggat ccatcttcat cgccatgtcg
    27961 gcgtcggtca tgatcggatt caagcgaatg ctcggatctt ccacgtccac cggtttgtct
    28021 tcttccttga tgttgatcgg ctcccactgc gtggcaccgg ccgggctctt tttcagttcc
    28081 cagttgtagt tcagcagaag gtggaagtaa ccgttgtccc actgggtcgg gttggtggtc
    28141 caggcgcctt caatgccgct ggtcacggtg tcgcggccca cgccacggct gacgtggttg
    28201 ttccagccca ggccttgttc ttcaatgtct gcaccctctg gctcagggcc gaggttcttc
    28261 gcattaccgt tgccgtgtgc tttacccacg gtgtgtccgc cggcagtcag ggccacggtt
    28321 tcttcgtcat tcatcgccat gcgggcgaag gtgatgcgca cgtcatgggc ggttttcagc
    28381 gggtcggact tgccgtccac gccttccggg ttgacgtaaa tcagacccat catcaccgct
    28441 gccagcgggt tggccaggtc gcgctcgccg gagtagcggc tgccttcgct gccggttggc
    28501 gccagccatt gtttttccga accccagtaa atgtcttttt ccgggtgcca gatgtcttcg
    28561 cggccaaagg cgaaaccgaa ggtcttcagg cccatggact cgtaagcgat ggtgccggcc
    28621 agggccagca ggtcagccca gctgacgcag ttgccgtatt ttttcttgat tggccagagc
    28681 aggcgacggg ctttgtccag gttggcgtta tccggccagg agttgagcgg ggcaaaacgc
    28741 tggctgccgc tgccggcacc accacggccg tcggcgatac ggtaagtacc ggcagcgtgc
    28801 caggtcagac gaatcatcag gccgccgtag tgcccccagt cggctggcca ccagtcctgg
    28861 ctgttggtca tcagcgcagt aaggtcacgc ttgagtgcgg cggcgtcgag ttttttcagt
    28921 tcttcgcggt aattgaagcc ctgaccgagc ggattggact tgctgtcatg ctggtgcagg
    28981 atgtcgaggt tcagcgcgtt aggccaccag gcgacgctcg attgatcatt ggccgtgttg
    29041 gcaccgtgca taacggggca ttggccggca gtgctttggc ttttcgggtc catatcaggc
    29101 tcctcaggtt tgtagatgcc cgatgctggg cgggtagctc agaacaggca tgttctcggt
    29161 tgccttgggt cgtcagagca gccgtttgtc gatttgccgt tgaggttagc agctaagaat
    29221 tgattaaagg gccgaaagga gattcgtatt ctctatgggc ttgttaggca gaattgcatg
    29281 ccgctagggc gcgagatttg ctcagctact tgcgtgttgc gatagcgctg ttgtgctccg
    29341 catgtgcaag cggaaatcag gcgcgaatcc agcgctgtcg gctccaactg ctcagcgcat
    29401 caacgccaaa taccagcagc aacatggcga tgatcaccgt gcttgcctga gactcctgaa
    29461 acaggctgag actgatgtag agcatctgcc ccagtccgcc ggccccgacg aagccgagca
    29521 cgctggccat gcggatattg ttttcccagc ggtacaggca gtaggccaac agctgtggcc
    29581 acaggttggg cagggtgccg tagcagaacg ccgcgacctg gctgccgccc tgcaggcgaa
    29641 tcgcttcggc gggtgcggtc ggggtgtttt ccagcgcctc ggcaaacagc cggccgagca
    29701 cgccagcggt gtgcagggcc agggccaggg tgccggcatt tgggccaagg cctgcggcca
    29761 gtaccatcag cgcggcccag actaattcgg gaatagcgcg cagggcattc agcagcaaac
    29821 gcgcggcgct ctgcagcggc cagccaaagc gcccggccgc aggcagcgcc agcaacaggc
    29881 cgaacaccgc tgccagcaag gtgccgaggg ccgacatggc cagggtttcc agggcgccac
    29941 ggccgatggc cgccaggtgc tcggcgctca ggtcggggct gagaaagcgc cgcacatagc
    30001 cagccatctg gcccaggcta tcgccactgg tcagcgcgcc caggtcgatg cccagataaa
    30061 cgaaggaggc gatcaccgct gcgccgatac caagcagcag caacaggttg atcagccggt
    30121 tcatgtcagc cgcccgcgca gcacacggct gagttggtcg gctagcagca ccagcagcag
    30181 gaaggtcagc agcatgctgg ccacttcacc accggcgaac atgcgcatcg acaggtcgat
    30241 ctgctggccc aggccgccgg cgccgacgaa gcccattacc accgaagcgc gaattgcgca
    30301 ttcccagcgg tacacggtgt aggaagtcag ttctgctgcg gcattcggca ggatgccgta
    30361 gacgaacgcc gccagccgac cgctgccaga ctgcagcagg gcgtgggccg ggcgctgatc
    30421 gaccgattca aagatttccg catagacctt gccgagcatg ccgctgtagg tgatggcgat
    30481 ggccagcacc ccggcggtag ggccgaggcc gaccgcgcgg acgaacagca gcgcccaaac
    30541 aatttccggc acgctgcgca ggaagatcag caggccgcgc accggccagc gcagcaactg
    30601 cgccagcggc ttaggtcgac cgccacggga ggccgctgac agcgacagcg cgcggctggc
    30661 tagcaggctg gctggaatcg ccagcagcag ggccagggcc ataccagcgg tggcgatggc
    30721 cagggtttgc aaggtagcgt cgagcagtaa ctgcagaaag tccgcgtcat gcgccggcgg
    30781 ccagaaggcc gcgacgaagc ggcccatctc gctctggctg tcggcttgca acagcacgct
    30841 caggtccagc tcgctgagct gaatacccgg ccacaacagg gccaacgcca gcagcgtgag
    30901 cagtaggcgg ggcagggcgg ccgggtcgcg gcttagcatc gcgggatctg caggctcaag
    30961 gtcacgctag gcgcgggcgg cgagagcagt tgctcattgg catacagcgc gtcgagttgc
    31021 gcctgatcga cttcgccggc agggcggtcg aacaggatct gcccgtcgcg caagccga
//
LOCUS       DASRUM010000114        12181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1066944, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000114 DASRUM010000000
VERSION     DASRUM010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 12181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12181
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066944"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1271
                     /gene="codA"
                     /locus_tag="VFF22_07725"
     CDS             33..1271
                     /gene="codA"
                     /locus_tag="VFF22_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012314099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HZX16738.1"
                     /translation="MNIVNARLRKKPGLYRIELHGERIAAITEQAALIDANSNDLDAA
                     GNLVTAPFIEPHIHLDATLTAGEPAWNMSGTLFEGIERWGERKAVVTHEDTKLRARKT
                     IDMLVDHGIQHVRTHVDVTDPTLAALKAMVEVREDVRHLIDLQIVAFPQEGIESFTNG
                     RALMEQAIALGADVVGGIPHFENTRDQGVSSIRFLMDLAERTGCLVDVHCDETDDPHS
                     RFLEVLAEEARVRGMGERVTASHTTAMGSYDNAYCSKLFRLLKQSKINFISCPTESIH
                     LQGRFDSFPKRRGLTRVAELDRAGMNVCLAQDSIVDPWYPLGNGNILRILEAGLHICH
                     MLGYEDLQRCLDFITDHSARTLNLGERYGLEVGRPANLLILSAPDDYEMLRSQGHALV
                     SIRAGKVLMQRTPAQISRAS"
     gene            complement(1353..2204)
                     /locus_tag="VFF22_07730"
     CDS             complement(1353..2204)
                     /locus_tag="VFF22_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HZX16739.1"
                     /translation="MRNDAHDELDHVPSLRADQRDHDHDDYAADHPEPARGNAYGRAA
                     VAAPVKSASTGPLWALVGALMIALGGLGWWSFQQISLMEQQLVATQESFARISEEAAG
                     RIQDISGKVVATESSVTSGSEALKLQVKQLESKLAELSKQQQGASSQQSEQGKRLEQL
                     AVQLKGQEGGGEKFEAALKKLNTEQAALKSEQATLKSALAGQSKLDEKLASLSGEIDA
                     LKKQGNPNQAIARVEQDLLVLKSDLETRSAGSSTAEFDAFRAQMTRNISTLQAQVQNL
                     QQQIDAR"
     gene            2530..2841
                     /locus_tag="VFF22_07735"
     CDS             2530..2841
                     /locus_tag="VFF22_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16740.1"
                     /translation="MELNREVLDCMKSLRRRLREEQAVDIRMSQPDAINSMLSACLTS
                     SNEETRRLGQLLAQYSDRPFGEPATATRMQAGHPLLVEDLAHRQASTSVRMYRGQRVY
                     A"
     gene            complement(2858..3850)
                     /locus_tag="VFF22_07740"
     CDS             complement(2858..3850)
                     /locus_tag="VFF22_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZX16741.1"
                     /translation="MKILVTGASGFIGGRFARFALEQGLNVRVNGRRAEGVEHLCSRG
                     AEFVQGDLSDPELVQQLCRDVETVVHCAGAVGVWGRYEHFYQANVTVTENVVDACLKQ
                     KVRRLVHLSSPSVYFDGQAHVGLREEQLPKRFSDHYGKTKFLAEQQVFAAQEFGLEVL
                     ALRPRFVTGAGDTSIFPRLINMQRKGRLSIIGNGLNKVDFTSVHNLNEALMSCLLAAG
                     PALGQVYNISNGAPVPLWDVVNYVLRKLDLLPVTRHLPYRLAYGAAALNEGVCKLLPG
                     RPEPTLFRLGVAVMAKDFSLDISRAQQYLEYEPKASLWSALDEFCAWWQAHHPS"
     gene            4020..5075
                     /locus_tag="VFF22_07745"
     CDS             4020..5075
                     /locus_tag="VFF22_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HZX16742.1"
                     /translation="MATHWMIYGANGYTGHLLAAEAKRQGLTPILAGRNPAAVHALGS
                     LLGLECRIFDIHQLDRAKEALADIAVVAHCAGPFSATSAPMLDACLASATHYVDITGE
                     ISVFEAAHQRDQQARELAVVVCPGVGFDVIPSDCLAACLHQALPDATHLALGFDSGSG
                     LSPGTAKTSVEGLKFGGKVRRAGVITDVPLGYKRRAINFGRGEKPAVTIPWGDVSTAY
                     HSTGIDNIEVYLPTPTAAAIVMRLIDPLRALLGLDVVQNWLKRLIEKRVKGPDEASRT
                     RQRTWLWGEARNAAGVVKTARLETANGYDVTVHGALLAVRHLLDYNGPGGYFTPSKLL
                     GERCVEQLPGSGKVVIS"
     gene            complement(5068..5961)
                     /locus_tag="VFF22_07750"
     CDS             complement(5068..5961)
                     /locus_tag="VFF22_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HZX16743.1"
                     /translation="MLDYKLLAALAAVVEQAGFERAAQVLGLSQSAVSQRIKLLEARV
                     GQPVLVRAVPPAPTEIGRRLLNHVQQVRLLERDIQAQVPGLDEGGLPERLRIALNADS
                     LATWWASAVGAFCTEQRLLLDLVVEDQEVGLKRMRAGEVAACVCASERPVAGARAVLL
                     GAMRYRAMASPAFISRHFPGGVTAQALAHAPAIVFGPDDLLQHRYLSGLGVEGGFVHH
                     LCPSSEGFIRLAQAGFGWGLIPELQVLGESDRGELVELISDRPIDVPLYWHHWRNGGE
                     LLNQLTRHLTQAAGQSLVMLS"
     gene            6064..6675
                     /locus_tag="VFF22_07755"
     CDS             6064..6675
                     /locus_tag="VFF22_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HZX16744.1"
                     /translation="MWQSYSNGLLIAAGLIIALGAQNAFVLAQSLRREHHLPVALLCV
                     VCDALLVTAGVFGLAAVLAQSPILLGVARWGGAAFLLWYGAQALVRASRPQALSQTSD
                     SGPRSRRTVLLAALAVTLLNPHVYLDTVLLIGSLGAQQPVPGAYALGAASASLMWFFC
                     LALGAAWLAPWLARPSTWRFIDLGVALMMFSIAWQLLSNPGML"
     gene            6852..7433
                     /locus_tag="VFF22_07760"
     CDS             6852..7433
                     /locus_tag="VFF22_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HZX16745.1"
                     /translation="MAFELPPLPYAHDALQPHISKETLEFHHDKHHNTYVVNLNNLVP
                     GTEFEGKTLEDIVKTSSGGIFNNAAQVWNHTFYWNCLAPNAGGQPTGALADAINAAFG
                     SFDKFKEEFSKVSIGTFGSGWGWLVKKADGSLALASTIGAGCPLTSGDTPLLTCDVWE
                     HAYYIDYRNLRPKYVEAFWNLVNWDFVAKNYAA"
     gene            complement(7498..8142)
                     /locus_tag="VFF22_07765"
     CDS             complement(7498..8142)
                     /locus_tag="VFF22_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16746.1"
                     /translation="MSLATRRRAIYTGLAGHFAEDELLAVLALWESKYADKPPFALNE
                     FLGEVAATTERKLERAKLYRELVGALTGPLSALLPDPEPLLLSWRQRMGVAAPLRTGP
                     DSQARHTFEALSRVLFNELEAELVPRLQRFAAGNLGALNVANEQRLRVRAWLEQGAAL
                     ESAGLGLEQLRQLLNLLYIGLCEYLGPVIADQRLSQAVQQVEALHLSFPPRKLL"
     gene            complement(8144..9439)
                     /locus_tag="VFF22_07770"
     CDS             complement(8144..9439)
                     /locus_tag="VFF22_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZX16747.1"
                     /translation="MKTRLLGIAVFAVSPLAQAASDEALNTAWLVLASVLVFFMQAGF
                     ALLESGMSRAKNAVNVMMKNYMDGCVGGITFWLLGFGLMFGSNITGWFGMSHFAPHTG
                     EPWDFTFLLFQMMFAATAATIASGAMAERTRYSAYLIGAVVISGFIYPVFGSWVWSGI
                     YGGQGWLAALGFIDFAGSTVVHSVGAWCALAGILVLGPRLGRFAADGTPRSIPGHNLS
                     LVALGGFILWVGWFGFNAGSSLEMNVSLGLIALNTHLAASAGALGAFLAQRSTSSPVL
                     LTTTVNGSLGGLVGITAGCATMSPLFALLTGLVAGFVVVFGMRLLERWQLDDVVGAVP
                     VHGFAGVWGTVAAGLFFSGDLFNWSRVGVQLLGAGVAFVWAFPLALMLYLLLAKTLGL
                     RTSTQHEQRGLDYAEHAEVGYPEFNLAVTFDSHDLPRRV"
     gene            9696..11759
                     /locus_tag="VFF22_07775"
     CDS             9696..11759
                     /locus_tag="VFF22_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZX16748.1"
                     /translation="MKLELKNSLSLKLLRVVLLSALIVGVVLSLAQIVFDVYKTRQAV
                     ANDAHRILGMFRDPSTQAVYSLDREMGMQVIEGLFQHESVRMAAIGHPNEPMLAEKAR
                     EQVDIPTRWLTDPILGKEQNFTTQLVGRGPYSEYYGDLSITLDTAPYGESFVTNSVII
                     FISGVLRALAMGLVLYLVYHWLLTKPLSKIIEHLTNINPDRPSEHKLPMLKGNEKNEL
                     GLWINTANQLLTSIERNTNLRREAENSLLRMAQYDFLTGLPNRQQLQQQLDQILEDAG
                     RLNRRVAVLCVGLDDFKGINEQFSYQTGDQLLLALSDRLRGHSGRLGALARLGGDQFA
                     LVQADIEQPYEAAELAQSVLDDLDNPFMLDQQQVRLRATIGITLFPEDGDSTEKLLQK
                     AEQTMTLAKSRSRNRYQFYIASVDSEMRRRRELEKDLRDALALNQLHLVYQPQVDYRD
                     HSVVGVEALLRWQHPQHGLVPPDLFIPLAEQNGTIIPIGEWILDQTCRQLREWHDQGF
                     TELRMAVNLSTVQLHHAELPRVVNNLMQVYRLPLRSLELEVTETGLMEDINTAAQHLL
                     SLRRSGALIAIDDFGTGYSSLSYLKSLPLDKIKIDKSFVQDLLEDEDDATIVRAIIQL
                     GKSLGMQVIAEGVETVEQEAYIIAQGCNEGQGYLYSKPLPARELTAYLKQARRLNNAA
                     NPATL"
     gene            complement(11991..>12181)
                     /locus_tag="VFF22_07780"
     CDS             complement(11991..>12181)
                     /locus_tag="VFF22_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16749.1"
                     /translation="RLAEARGIRSMAESFAGESKHPRKRVQKPKHNAKDFHFAQRVNA
                     AFLPRAVGGVCDRAIRYG"
BASE COUNT         2543 a         4042 c         3459 g         2137 t
ORIGIN      
        1 ctctcgccgc cgcgaaacac gatgaatact ggatgaatat cgtcaacgcc cgcttgcgca
       61 agaaacccgg cctttaccgc atcgaactgc acggcgaacg cattgccgcg atcaccgaac
      121 aagcggctct tatcgacgcc aacagcaacg acttggacgc cgccggcaat ctggtgacag
      181 cgccctttat cgaaccgcac atccacctcg acgccaccct caccgccggc gagccggcct
      241 ggaacatgag cggcaccttg tttgaaggga tcgagcgctg gggcgagcgc aaagccgtgg
      301 tcacccacga agacaccaag ctgcgcgccc gtaagaccat cgacatgctg gtcgatcacg
      361 gcatccagca tgtgcgaacg catgtcgacg tgaccgaccc gaccctcgcc gcactcaagg
      421 cgatggtcga ggtgcgcgaa gacgtgcgcc acctgattga cctgcagata gtcgcctttc
      481 cccaggaggg catcgagtca ttcaccaacg gccgcgcctt gatggaacag gcgattgccc
      541 tgggtgccga tgtggttggc ggcattccgc actttgagaa cactcgcgac cagggtgtca
      601 gctcgatcag gtttctgatg gatttggccg agcgcaccgg ctgcctggtg gatgtgcatt
      661 gcgacgaaac cgacgacccg cactcgcgct ttctcgaagt gctagctgaa gaagcacgcg
      721 tacgcggcat gggcgagcgc gtcaccgcca gccacaccac ggccatgggc tcttacgaca
      781 acgcctattg ttcgaagcta tttcgcctgc tcaagcagtc gaagatcaat tttatctcct
      841 gccccaccga gagcatccac ctgcaaggcc gctttgacag cttccccaag cgccgcggcc
      901 taactcgcgt agccgagctc gatcgtgctg ggatgaacgt ctgcctggcg caggactcca
      961 tcgtcgatcc ctggtaccca ctgggcaacg gcaacattct gcgcattctc gaagccggac
     1021 tgcacatctg ccacatgctc ggctacgagg acctgcagcg ctgcctggat ttcatcaccg
     1081 accacagcgc ccgcaccctc aatctgggtg aacgctatgg actggaggta gggcgcccgg
     1141 ccaacttgct gattctgtcc gcgcctgatg actatgaaat gctgcgtagt cagggccacg
     1201 cgctggtgtc gatacgagcc ggcaaggtgc tgatgcagcg cacacctgcg cagatcagcc
     1261 gcgccagcta ggcatagggt agcggatgcg acgcctcgcg ctgaggcgcg acaggccgcc
     1321 cacgagcagg gcggccgcgc cgaagcaaac gattagcggg cgtcgatctg ctgttgcagg
     1381 ttctgcacct gcgcctgcaa ggtgcttatg ttgcgggtca tctgcgcgcg gaaggcgtcg
     1441 aactcggccg tgctgctgcc ggcggaacgg gtttccagat cgcttttcag caccagcaga
     1501 tcttgctcga cgcgggcaat ggcctggttg gggttgccct gtttcttcag cgcatcgatc
     1561 tcgccgctca agctggcaag cttctcgtcg agtttgctct gcccggccaa cgcactcttc
     1621 agggtggctt gctcgctttt cagcgcagcc tgctcggtgt tgagtttctt caaagcggct
     1681 tcgaactttt caccgccgcc ttcctggccc ttcagttgta cggccagctg ttcaagacgc
     1741 ttgccctgct cgctctgctg gctgctggcg ccctgctgtt gtttgctcag ctccgctagt
     1801 ttgctttcca gttgcttgac ctgcagcttc agcgcttcgc tgccactggt gaccgaagat
     1861 tcggtggcca ccaccttgcc ggaaatgtcc tgaatccgcc cagccgcttc ctcgctgatg
     1921 cgcgcgaaac tctcctgggt ggcaaccagt tgctgctcca tcaggctgat ctgctgaaag
     1981 ctccaccagc cgagcccacc cagggcaatc atcaaggcgc caaccagggc ccagagcggg
     2041 ccggtgctgg ccgacttcac cggtgcagcg accgccgcgc ggccataggc attgccgcga
     2101 gctggctccg ggtgatcagc cgcgtagtca tcatgatcgt gatcgcgctg gtcagcccgc
     2161 aggctgggca cgtgatcgag ttcgtcgtgg gcatcgttac gcatgggtca accttcaaga
     2221 aagcgcgggg atagcgaatt gccgcgcagt ataacggttc gcgcgcagcg cttcagtgtc
     2281 gcgccaagcc agtgcgaaat gcaccgacga agggcatggc acaggccatc gactcgctac
     2341 aggccgcgca gttccacaaa gtgtgaactg gtatgcgctt tgcacttgtc tatttgcgac
     2401 gttttcgcag aaccggggag gcacaggtgc tgcaggcgct atccgcacgc gggaaacgcg
     2461 gcgagaccag gcaggtggca ttccggatgg atgcaaccta gcccacttgt aataacgggg
     2521 gaaaggtcca tggagctgaa cagagaagta ctggattgca tgaagagcct gcgccgccgg
     2581 ctgcgcgaag agcaggcggt ggatattcgc atgagccagc cagacgcgat caacagcatg
     2641 ctcagcgcct gcctcacctc aagcaatgag gagacccgtc gactggggca actactggcc
     2701 cagtacagcg accgcccctt cggcgagccc gcgactgcaa cgcgcatgca ggccgggcac
     2761 ccgctgctgg tggaagacct ggcgcatcgt caagccagca cctcggtacg gatgtatcgc
     2821 gggcagcgcg tctacgcctg agccgcgcct cgcgcagtca gctcgggtga tgcgcctgcc
     2881 accaggcgca gaactcatcc aacgcactcc ataagctggc cttgggttcg tactccagat
     2941 actgctgcgc gcggctgata tccagggaga aatccttggc catcaccgcc acgcccaggc
     3001 ggaacagcgt cggctccggg cgaccgggca gcagcttgca cacgccttca ttcaacgccg
     3061 ccgcaccata tgccaggcga tagggcagat gacgggtgac gggcagcagg tcgagtttgc
     3121 gcagcacata gttgaccacg tcccacagcg gcactggcgc gccattgctg atgttgtaaa
     3181 cctgccccag cgccggcccg gccgccagca ggcagctcat cagcgcttcg ttgaggttgt
     3241 gaacgctggt gaaatcaacc ttgttcaggc cgttgccgat gatcgacaac cgccccttgc
     3301 gctgcatatt gatcaggcgc ggaaagatgc tggtatcgcc cgccccggtg acaaaacgcg
     3361 ggcgcagcgc cagcacttcc aggccgaatt cctgggcggc aaacacttgc tgctcggcga
     3421 gaaacttggt tttgccgtag tgatcggaaa agcgcttggg caactgttct tcacgcagcc
     3481 cgacatgcgc ctgaccatcg aaatacaccg acggcgacga caggtgcacc agccgccgca
     3541 ccttctgttt caggcaggca tccaccacat tctcggtgac cgtgacattg gcctgataga
     3601 agtgttcgta acgcccccag acgccgaccg cgccagcgca gtgcaccacc gtttcaacat
     3661 cccggcacag ctgctgcacc agctccgggt cggacagatc accctgcaca aactccgcgc
     3721 cacgcgagca cagatgttca acgccctcgg cgcgccgccc gttgacccgc acgtttaggc
     3781 cctgctccaa ggcaaagcga gcgaaacgcc caccgataaa accactggcc ccggttacca
     3841 gaatcttcat tgccgccccc tttaccgtga ttgcaggcca ctctagcagc gcaggctggg
     3901 cgctgccgtg gcattgcaag ccagaacaac gactgcgcac accctgcgat aggccaacag
     3961 gcccgtccac agcggctaag ctgccagacg caaacctaat aacaactaag gagcacggga
     4021 tggcgacgca ctggatgatt tacggagcca acggttacac cggccacctg ctggccgccg
     4081 aagccaagcg ccagggcctt acgcccattc tggcggggcg caacccggcc gccgtacacg
     4141 ccctgggcag cctgctgggc ctggaatgcc ggatattcga tatccaccaa ctggatcgtg
     4201 ccaaggaagc cctggccgat attgccgtgg tcgcccactg cgccgggcca ttctctgcaa
     4261 ccagcgcgcc gatgctcgac gcctgcctgg ccagcgccac ccattacgtc gatatcactg
     4321 gtgaaatcag cgtgttcgaa gccgcccatc aacgtgatca acaagcgcgc gaactggccg
     4381 tggtggtctg ccccggcgtg ggcttcgatg tgattcccag cgattgcctc gccgcctgct
     4441 tgcaccaggc tctgcccgat gccacgcacc tggcgctggg cttcgactct ggcagcgggc
     4501 tgtcgcccgg caccgcgaaa acctcggtgg aaggcctgaa atttggcggc aaggtacgcc
     4561 gcgcaggtgt gatcaccgat gtgccactgg gctataaacg ccgcgctatc aactttggtc
     4621 gtggtgaaaa gcctgcggta accattccct ggggcgatgt atccaccgcc taccactcca
     4681 ctggtatcga caatatcgag gtgtacctgc ccacaccgac agccgccgcg atcgttatgc
     4741 gcctgatcga cccgttgcgt gccctgctgg gtctggatgt ggtgcagaac tggctcaagc
     4801 ggctgatcga aaaacgcgtc aaaggccctg atgaagccag tcgtacgcgc caacgcacct
     4861 ggctgtgggg cgaggcacgc aatgccgcag gcgtggtgaa aaccgcgcgc ttggaaaccg
     4921 ccaatggcta tgacgtaacc gtacacggcg ccttgctggc cgtgcggcac ttactcgatt
     4981 acaacggccc cggcggttat ttcaccccca gcaaactgct cggcgagcgc tgcgtggaac
     5041 agctgccagg ctcgggcaag gtggttatta gctaagcatc accaggcttt ggccggcggc
     5101 ctgggtaagg tgacgcgtca gctgattaag caactcgccg ccattacgcc aatggtgcca
     5161 gtacagcggc acatcaatcg gccgatcact gatcaactcg accaactcgc cccggtccga
     5221 ctcccccagc acctgcagct cggggatcag cccccagcca aacccggcct gcgccaggcg
     5281 gataaagccc tcggatgacg ggcacaaatg gtggacaaac ccgccttcca cgcccagtcc
     5341 ggacaggtaa cggtgctgca gcaagtcatc cggaccgaag acgatagccg gcgcatgggc
     5401 cagcgcctgc gccgtcaccc cacccggaaa atgccgggaa ataaacgcgg gactggccat
     5461 ggcgcggtaa cgcatcgccc ccagcagcac ggcgcgcgca ccggctaccg ggcgctcact
     5521 ggcgcatacg caggcggcca cttcgccggc gcgcatacgt ttgaggccaa cttcctgatc
     5581 ctccaccacc agatccagta gcaaacgctg ctcggtgcag aacgcaccca ccgcgctagc
     5641 ccaccaggtg gccaggctgt cggcattcag cgcaatgcgc aggcgctcgg gcaagccgcc
     5701 ctcatccagc cccggcacct gcgcctgaat atcgcgctcc agcaaacgca cctgctgcac
     5761 atggttaagc aaacgtcgac cgatttccgt cggtgcgggc ggcaccgcac gcaccagcac
     5821 tggctggccg acccgcgcct cgagcaactt gatccgctgc gacaccgccg attgcgacaa
     5881 gcccagcacc tgcgcggccc gctcgaagcc ggcctgctcg acaacagcag ccagggcggc
     5941 gagtagtttg taatccagca aaataagatt tcctaatgag tgatcaggat tattggtttc
     6001 tcttatatac ccagccacag cagactggcc agcattgaac aggcgagcgg cggagaacac
     6061 ctgatgtggc aaagctatag caatggatta ctgatcgccg ccgggctgat catcgccctc
     6121 ggcgcgcaga acgccttcgt cctggcgcaa agtctgcgcc gcgagcatca cctaccggtg
     6181 gcattactct gtgtggtctg cgatgcccta ctggtcactg ccggggtgtt cggcctagcc
     6241 gccgtgctgg cgcagagccc gattctgtta ggcgtggcgc gttggggtgg cgcggccttt
     6301 ctgctctggt atggcgctca ggcgctggtt cgcgccagcc gtccgcaggc actgagtcag
     6361 accagcgaca gcggcccccg ctcacgccgt acggtgctgc tggcggctct ggcagtaacc
     6421 ctgctcaacc cgcacgttta tctcgacacc gtgctgctga tcggctcgct cggcgcgcaa
     6481 caaccggtgc ccggcgccta tgccctgggc gcggccagcg cttcattgat gtggttcttc
     6541 tgcctggccc ttggcgcggc ctggctcgcg ccgtggctgg cgcgcccgag cacctggcgc
     6601 tttatcgacc tgggcgtggc cctgatgatg ttcagcattg cctggcagct attgagcaac
     6661 cccgggatgc tctaaaacgg ccctgcagcg acacgccgag gtagttttcc acacagttgc
     6721 cgcgtgttta aggcgcagtg ccggtgctat gatccaagac ctgcggcgca aagagataac
     6781 tcacgccgct tagatagccg cccgtgatcg gcttcgcgca caccgcccct gacctgatta
     6841 ggagataaac catggctttc gaattgccgc cgctgcctta cgcacatgac gccctgcagc
     6901 cgcacatttc caaggaaacc ttggagttcc accacgacaa gcaccacaac acctacgtcg
     6961 tgaacctgaa caacctggtc ccaggtaccg agtttgaagg taaaactctg gaagacatcg
     7021 tcaagacctc ttccggtggc atcttcaata acgccgctca agtctggaac cacaccttct
     7081 actggaactg cctggcgcca aacgccggtg gccagccgac tggcgccctg gctgacgcga
     7141 tcaacgcagc cttcggttcc ttcgacaagt tcaaggaaga gttcagcaaa gtttccatcg
     7201 gtaccttcgg ttccggctgg ggctggctgg tgaagaaggc tgacggttcc ctggctctgg
     7261 ccagcaccat cggcgccggc tgcccgctga ccagcggcga caccccactg ctgacctgcg
     7321 acgtctggga acacgcctac tacatcgact accgcaacct gcgtccgaag tatgttgagg
     7381 cgttctggaa cctggtcaac tgggacttcg tagcgaaaaa ctacgccgcc taagcacctg
     7441 cgttacccac aagaaacccg gcctagcgcc gggtttcttg ttatgtggag aagcgtttta
     7501 cagcagcttg cgcgggggaa aagacaagtg cagcgcctcc acctgttgca ccgcctgact
     7561 cagacgctga tcagcgatga ccggccccag gtattcgcac agcccgatat acagcaggtt
     7621 gagcaactgc cgtaactgct caaggcccaa gccagcggac tccagcgcag caccctgctc
     7681 cagccaggcg cgcacccgca ggcgctgctc gttagccacg tttaaagcgc caagattgcc
     7741 cgcagcaaag cgctgcaaac gcggtacaag ctcggcctcc agctcattga acaacacccg
     7801 gctcagcgcc tcgaaggtat gccgcgcctg gctatcgggc cccgtccgca gcggcgccgc
     7861 cacgcccatc cgctgccgcc agctaagcag cagcggctcc gggtcgggca gcaaggccga
     7921 taacggcccg gtcagcgccc ccaccagttc gcggtagagc ttggcgcgct ccagtttgcg
     7981 ttcagtggtc gcggccactt caccgagaaa ctcattgagg gcgaacggcg gcttgtcggc
     8041 gtatttactt tcccacagcg ccagcacggc cagcagctcg tcctcggcaa agtgcccggc
     8101 cagcccggtg tagatcgctc gtcggcgagt tgccaggctc atctcacacc ctcctcggca
     8161 agtcgtggct gtcgaaggtc acggccaggt tgaactcggg atagcccacc tcggcatgct
     8221 cggcataatc caggccgcgc tgctcatgct gcgtggaggt acgcaggccg agggtttttg
     8281 ccagcaatag atagagcatc agcgccagcg ggaaggccca gacaaacgcc acaccggcac
     8341 cgagcagctg cacgccgacc cgcgaccagt tgaacagatc gccactgaag aatagcccag
     8401 ccgccacggt gccccagacg ccggcaaaac catgcaccgg cacggcgccg accacatcat
     8461 cgagctgcca gcgctccagc agacgcatgc cgaacaccac cacaaaaccg gcgaccagcc
     8521 cggtcaacag ggcaaacagc ggcgacatcg ttgcgcaacc ggcggtgata cccaccaggc
     8581 cgccgagcga accgttcacc gtagtggtca gcagcaccgg gctggaggtg ctgcgctgcg
     8641 ccagaaatgc gcccagcgct ccggcagatg ccgccagatg ggtgttcagg gcaatcaagc
     8701 cgagactgac attcatctcc aggctgctgc cggcgttgaa gccaaaccag ccgacccaga
     8761 ggataaagcc acccagcgcg accaggctga ggttatgccc gggaatgctg cgcggcgttc
     8821 cgtccgctgc aaaacggccc agtctcggcc ccagcaccag aattccggcc agcgcacacc
     8881 aggcgccgac gctgtgcacc acggtcgaac cagcgaaatc gataaagccc aaggccgcca
     8941 accaaccctg gccgccatag atgccgctcc acacccagct gccgaacacc gggtagataa
     9001 acccgctgat caccaccgcg ccaatcaggt aggccgaata acgcgtacgc tcggccatag
     9061 cgccgctggc aatggtcgct gcggtggcag cgaacatcat ctgaaacagc agaaaggtga
     9121 aatcccaagg ctcacccgta tgcggcgcga aatggctcat gccgaaccag ccggtgatat
     9181 tgctgccgaa catcaggccg aagcccagca gccagaaggt gatgccgccg acgcagccgt
     9241 ccatgtaatt tttcatcatc acattgaccg cattctttgc tcgcgacata ccactttcca
     9301 gcaaggcaaa gccagcctgc ataaagaaca ccagcacact ggccaacacc agccaagccg
     9361 tattgagcgc ttcatcgctg gccgcctgcg ccagtggcga aaccgcaaaa acagctatcc
     9421 ctaacagtcg agttttcatc gcagcgtccc ccatgacggt gagtacaggg ggcttcgcaa
     9481 ctctcatgcc aatggaaaac cgccccacgg tgctcaagcc ataagtaact cgcaccgaca
     9541 cagtgcaggc agccaacaca gctgtcagcg cccgcgccat cgccgcgcag ataatttgtt
     9601 cgacaaagtt tggtaatggc cctttgactg caagcgcgac tttgccaata ctcactagcg
     9661 tctgacgccg aaggcctcga acaaggaacc gtcatttgaa gctggaactt aaaaacagct
     9721 tgtctctcaa gttgctccgc gtagtgctgt tatcagcgct gatcgtcggc gtggtcttga
     9781 gtcttgccca gattgttttc gacgtttaca aaacacgtca ggcagttgcc aacgacgcgc
     9841 accgaattct cggcatgttc cgcgacccat ctacccaggc ggtctacagc ctagaccggg
     9901 aaatgggcat gcaggtgatc gaaggcctgt tccagcacga gtcggtgcgc atggccgcca
     9961 tcggccaccc caacgaaccg atgctggcgg agaaagcccg agagcaagtc gacataccga
    10021 cgcgctggct gaccgacccg atccttggca aagaacagaa cttcaccacc cagctggtcg
    10081 gccgcggccc ctacagcgag tattacggcg acctcagcat caccctcgac acggccccct
    10141 acggtgagag ctttgtcacc aactcggtga ttatcttcat ctcaggtgtg ctacgcgcct
    10201 tggccatggg cctagtgctg tatctggtct atcactggct gctgaccaaa ccactgtcga
    10261 agatcatcga gcacctgacc aacatcaacc cggaccgccc cagtgagcac aagctgccga
    10321 tgctcaaggg taacgagaag aacgaactgg ggctgtggat caacaccgcc aaccaactgc
    10381 tcacctccat cgaacgcaac accaacctgc gccgcgaggc ggaaaacagc ctactgcgca
    10441 tggcccagta cgacttccta accggcctgc cgaaccgcca gcaactgcaa cagcaactcg
    10501 accagattct cgaagacgcc ggccgcctca atcgccgcgt ggccgtactg tgcgtgggcc
    10561 tcgatgactt caaagggatc aacgagcagt tcagttacca gactggcgac caactgctgc
    10621 tggctctatc tgatcgtcta cgcggccaca gtggccgcct cggcgccctg gcccgtttgg
    10681 gtggcgacca gttcgccctg gtgcaggccg atatcgaaca gccttacgag gcagccgaac
    10741 tggcgcaaag cgtgctcgac gacctcgaca acccgtttat gctcgaccag cagcaagtgc
    10801 gcctacgcgc taccatcggc atcaccctgt tcccggaaga tggcgacagc accgaaaagc
    10861 tgctgcagaa agccgaacag accatgaccc tggccaagag ccgttcgcgc aaccgttacc
    10921 agttctatat cgccagcgtc gacagcgaaa tgcgccgccg ccgcgagctg gaaaaagatc
    10981 tgcgcgacgc cctggcactg aaccaactgc atctggttta ccagccacag gtcgactacc
    11041 gcgaccacag cgtagtaggc gttgaagcac tgctgcgctg gcaacacccg caacatggtt
    11101 tagtgccgcc ggatctgttt atcccgctgg cagaacagaa cggcaccatc atcccgattg
    11161 gcgaatggat tctcgaccaa acctgccgcc agctgcgcga atggcatgat cagggcttca
    11221 ccgagctgcg catggcagtc aacctgtcca ccgtgcagtt gcaccacgcc gagctgccac
    11281 gggtggtcaa caacctgatg caggtctatc gcctgccgct gcgtagcctg gagctggaag
    11341 tcaccgaaac cggcctgatg gaagacatca acactgccgc ccagcacctg ctcagcctgc
    11401 gccgctccgg tgcgctgatc gccatcgatg acttcggtac tggctactcc tcgctgagct
    11461 atctgaagag cctgccgctg gacaagatca agatcgacaa aagcttcgtg caggacctgt
    11521 tggaagacga ggacgacgcg accatcgtgc gcgccattat ccaactgggt aagagcctgg
    11581 gcatgcaggt catcgccgag ggtgtggaaa ccgtcgagca ggaggcctac atcatcgctc
    11641 agggttgtaa cgagggtcag ggctacctgt acagcaagcc gctgccggcc cgcgagctga
    11701 ctgcctacct gaagcaggcg cgacgactca acaacgccgc taaccccgcc acgctgtaac
    11761 gtcatatgtc cacgaaaaag cccgcctgaa ttagcgggct ttttcatgca cggcgttttc
    11821 gcctagaaac tattgatacc caataaccat gccgctaatg cgctgctgca tatgctggaa
    11881 gcttgcatcg gctttcagct ccagggcgtt cgaacagctg accaatggca acacgggtaa
    11941 ggctaatgcg gaactcctga gtggctgttg aaagccgcag agcaggtatt ttagccatat
    12001 cgaatggccc gatcgcagac tccgcccaca gctcgcggta aaaaagctgc attgaccctt
    12061 tgcgcaaaat gaaaatcttt cgcattatgt ttcggttttt gtacccgctt gcgtgggtgc
    12121 ttactctcac cagcaaagga ttccgccatg ctccgtatgc ccctggcctc tgccagcctg
    12181 c
//
LOCUS       DASRUM010000115        28885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1237747, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000115 DASRUM010000000
VERSION     DASRUM010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 28885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28885
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237747"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..527)
                     /locus_tag="VFF22_07785"
     CDS             complement(180..527)
                     /locus_tag="VFF22_07785"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HZX16750.1"
                     /translation="MSSNIHNVQRIERGISIATGAAAIVSGVHQGGSAGLIKALGGLA
                     LVHRGISGHCSLKAMLTDPHAELVYLRQRVADLRAAVDKLEKNTSSGKRAQEAKVDHA
                     LEETFPASDPISP"
     gene            complement(697..1845)
                     /locus_tag="VFF22_07790"
     CDS             complement(697..1845)
                     /locus_tag="VFF22_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HZX16751.1"
                     /translation="MTDRSPPTFGIEEEYFLTDLLSRRMPRQPVEGFAAACQMALGNL
                     VTREMFAAQFEVVTPVLHNLDDARECLSSARRTLAQVAREFGFGLVAAGTQPLAQWRQ
                     QRPTGVARYQQIFDDFQMVASRSVLAGLHVHVGVSAELDRIRIMNRVTPWLPLLLGLS
                     ASSPFWEGKPSGLMSYRQAVCDEWPRMGIPDHFDNDADYQRYVQMLLDTGSIRTTADV
                     WWNIRPSVRYPTLELRIADACPQLEDALCIAALFRAMVNYATHMPSAAWIDDPITRVI
                     TLENRWRAKRYGLHGAFIDPTTQRALPFAEWLGSVLQQLQAHIAPGDESAVQHAQRLV
                     RDGGSAQAQLHQYQQERDAGRSHGEALMAVVDALMAQTELRDVGRGGE"
     gene            2038..3447
                     /locus_tag="VFF22_07795"
     CDS             2038..3447
                     /locus_tag="VFF22_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HZX16752.1"
                     /translation="MSILSLVDTSSSNHAPPLTGSGSWQQIYRHLLAEPLDAREAARD
                     FLNERLEEAADLPCELPERPEQLHAWSLRGSASVTQAYAAYLDKRRAGGARQYFRHRA
                     HALHFLRAVAPTKLVDGAWLYGTLPHWRDVRVQPLVRTYLEELGDGVPQQNHVLLYRR
                     LLTAQGCDDLNNLDDALYLQGAIQLALGHLGSDYLPEVIGYNLGYEQLPLHLLISAFE
                     LEELDIDPYYFLLHVTIDNSGTGHAAKAVRAVLDNLPLLGDAKAFYRRVALGYRLNDL
                     GVSSTRIIAGFDLEQELHAMLEHKRGVAGQVHSDYCRIEGRTINQWLACQEGIGGFLD
                     ALQRRGWINRHQDPILSRFWNLVRGDGAAMFGVFSAYEQQLVHDWIAGDWLDTPTAKH
                     LDTALFRKNRQRQDPLLEPASAPASSELDNEQQLLRKQLQRMSAAQREARLIELLAPV
                     YHSTSAGLAATRIFAALAY"
     gene            3462..4475
                     /locus_tag="VFF22_07800"
     CDS             3462..4475
                     /locus_tag="VFF22_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX16753.1"
                     /translation="MYEALAPLASPQALALLRLGAALSARGYRFITPTPATQQRVNLR
                     PGNEQARNLRDIFGWSRPFRRETLDAELFELMRRADVLQAQGERWLSRVRWSSLGDQL
                     FVHSRFPTDSADAVFFGPDTYRFVRMVRDHLQSHSHPLQRIVDIGCGAGPGALTAAML
                     QPQAEVLALDINDQALAFTAVNACLGGVSNLVTRRSDLLRDVTGNFDLIIANPPYMLD
                     AQQRTYRHGGGKHGIGLSLKIFDAALERLTPGGTLLLYTGVAIFDNEDPFLNAIRPSL
                     ADINWGWSYEEIDPDVFGEELEKPAYAYAERIACVALKLTRPFPTAGADEQARQAALL
                     QSS"
     gene            complement(4670..5527)
                     /locus_tag="VFF22_07805"
     CDS             complement(4670..5527)
                     /locus_tag="VFF22_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX16754.1"
                     /translation="MDDKQTLPAQHQDRQPGLEHDMHPQPTYLSTDYRAANKLAGKVA
                     IITGGDSGIGRAVAVHFALEGAHVALVYLDENEDAQKTLSEITKHGGQAIALAGDIAQ
                     EGFCKCVVDAVIAKWGRLDILVNNAGEQHPESNLEDMDEQQWEKTFRTNIFAMFQLTK
                     LALAHMQAGASIINTSSVTAYKGNPMLLDYSSTKGAITSFTRSLSTNLAAKGIRVNGV
                     APGPIWTPLIPSTFSAEKVATFGADTPMGRPGQPSEVAPAFVYLASNDASYMTGQMLH
                     VNGGSVVNG"
     gene            complement(5632..6495)
                     /gene="galU"
                     /locus_tag="VFF22_07810"
     CDS             complement(5632..6495)
                     /gene="galU"
                     /locus_tag="VFF22_07810"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HZX16755.1"
                     /translation="MIKKCLFPAAGYGTRFLPATKSMPKEMLPVVDTPLIQYGVEEAL
                     EAGLNEIAVVTGRGKRSLEDHFDISYELEHEISNTSKEKSLSGIRRLIDECSFSYTRQ
                     IEMKGLGHAILCGRPLIGDEAFAVVLADDLCLNVDGDGVLTQMVKLYEQFRCSIVAIQ
                     EVPLEEVSRYGVIAGEAIRDDIFRVERMVEKPKPEDAPSNLAIIGRYILTPDIFALIE
                     QTVPGKNGEIQITDALMKQAQQGCVMAYKFKGQRFDCGSAEGYVEATNFCFENIYKSK
                     TASHAPRLEVV"
     gene            6875..7189
                     /locus_tag="VFF22_07815"
     CDS             6875..7189
                     /locus_tag="VFF22_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16756.1"
                     /translation="MKVEELTITSEGYAVQATQRPDGSILLKFSSSNGKTIERVAGKI
                     TDIGEAVRAVKFDIAYEAGNSCLHDAVKYCSSTAGLPTYMRTPLFRTHSSRLWSTRKL
                     RE"
     gene            7233..7604
                     /locus_tag="VFF22_07820"
     CDS             7233..7604
                     /locus_tag="VFF22_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16757.1"
                     /translation="MDILNRERRLMSKFCEWFARPETTTFIAFMFIFNLKLLFPLEVS
                     SEPQKLLSMHYAESNCDFKNQLKAQAACVGNCPDQDESGWNLNRLTVTCEQGVIEGAT
                     GWHQRGMDSMVIQEIKRMPIF"
     gene            complement(7642..8103)
                     /locus_tag="VFF22_07825"
     CDS             complement(7642..8103)
                     /locus_tag="VFF22_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HZX16758.1"
                     /translation="MPTLDKTIEQLNQCLVTCYSARLIFQDFAKISHAEELRYLFTSI
                     ALTLPLLGEQLQQQIRSYGGRSSRHASVIGMLHCGWQWLKLRIGKMDDRDLLNEGLRV
                     ERAVCQRFETLLQGDLPPVFRDQLMVHYGVLLDQQAQLQALLQKWRNVERR"
     gene            8407..8802
                     /locus_tag="VFF22_07830"
     CDS             8407..8802
                     /locus_tag="VFF22_07830"
                     /inference="COORDINATES: protein motif:HMM:NF039524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16759.1"
                     /translation="MHNPNDTNREGVSALGKGTGPIVAGPTARGETKLGSPDVAHDFH
                     NFLADIEDLIKDVTTLTGEDLAQAKAKLSSRIDAAKVSAEAVGESLSQRAKQTVASTN
                     DYLHEKPWTALCAGAAIAFLLGLLTARRN"
     gene            complement(8916..9710)
                     /locus_tag="VFF22_07835"
     CDS             complement(8916..9710)
                     /locus_tag="VFF22_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZX16760.1"
                     /translation="MNQPIKKIWDARVAPENTHQNLLLASLSKDVQERLFSRMEWVEL
                     PQGKVVYESGDTVRHVYFPTDAIIALLCVTESGASAETALVGNEGVAGLALLMGGGSM
                     STQAVVQNSGHAYRLPGQRFMDECDRHGELLQLVLRYTQTLITQMSQTAVCNRHHCIE
                     QRLCCWLLLSLDRLPGTELAMTQELIASMLGVRREGVTEAAGKLQRQGIIEYYRGRIT
                     VLDRTRLEHLSCECYAVVRKETDRLLPGRPAQLNAVTYRRVGASAG"
     gene            10108..10311
                     /locus_tag="VFF22_07840"
     CDS             10108..10311
                     /locus_tag="VFF22_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16761.1"
                     /translation="MINQRKPYSANPTSPSQPSAGSQQKPDFKEEQTATRAWPTVHKQ
                     FRIVAASRPVPGNASKETSGLAE"
     gene            10419..11546
                     /locus_tag="VFF22_07845"
     CDS             10419..11546
                     /locus_tag="VFF22_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HZX16762.1"
                     /translation="MTFDWLSYIPTPDLQVLLAALLVALVLAFATKWLFLLLLRVTHS
                     LTFTRELLLQAKAPLRLLLPLLGIHTVWNSSPDEIFFIDAARHLTSLAIIGSVTWLGL
                     RAVDAVQQIILVHHPVNVSDNLRARRIQTQTRVLVRTLSALVMIFGFAAMLMTFPSAR
                     QFGASLLASAGLAGLAVGFAAKPVLGNLIAGLQIALTQPIRLDDVVIVENEWGRIEEI
                     TGTYVVVRIWDDRRLIVPLQWFIENPFQNWTRMTSSLIGAVTLWVDYSTPLEPLRQEL
                     ARLCNEVPELWDGRVSVLQVIDSNERGIQLRVLASSGDSSRNWDLRCYIRENLINFIN
                     RDYPHCLPQLRGVLKPDVTKEHRDELPAQGDISEPERQPPV"
     gene            complement(11551..12033)
                     /locus_tag="VFF22_07850"
     CDS             complement(11551..12033)
                     /locus_tag="VFF22_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HZX16763.1"
                     /translation="MQSVEQVVRFLEKYQLSLTTAESCTAGLMASMVAEVMGAGAVLE
                     SGFVVYSPEAKHACLGVSLQTMETFGLTSEEVAREMAIGAMKLSKANIVLANTGLAEA
                     AGEMDGVQCFACAMKVNGHEGVVSETVKFAGERNEVRVAASRYALLQLPYYYERLRQV
                     "
     gene            12393..13310
                     /locus_tag="VFF22_07855"
     CDS             12393..13310
                     /locus_tag="VFF22_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005754452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HZX16764.1"
                     /translation="MVSAMLFMLVKNLDWQDLKQAVQSFKASTLLLCILITMLSYSLF
                     SCYDLLGRIYTGHKLPAKQILPVAFVCYAFNLNISAWIGSIALRYRLYSRLGLDLATI
                     TRVFSFSLVTNWVGYTLIGGIVFSLRLLELPANWSIGTVGLQLIGIAMLAVSITYLLA
                     CRFAKRRAWSFRQHELTLPSLRLALLQVAMATLNWSLSSLVVFLLLPDKVTYATVIGI
                     MLISSIAGVITHIPAGLGVLEAVFIGLLQHEISKGSILAALIGYRAIYLLLPLLLACV
                     VYLVLEKRAKTLRRRTHLRQSGNAICSHK"
     gene            complement(13387..15846)
                     /locus_tag="VFF22_07860"
     CDS             complement(13387..15846)
                     /locus_tag="VFF22_07860"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HZX16765.1"
                     /translation="MPDETSSDADGVKAFKNSILAKLKYAVGRDPSSAFDHDWFEAVA
                     LAARDHVVDRWEEASAEVERHPPKRIYYLSLEFLIGRLLVDNLSNLGLLDTARQALAE
                     LNVDLEQIRQFEPDAALGNGGLGRLAACFMESMATLGLAAHGYGIRYEHGLFRQAIAD
                     GWQLEQTETWLDFGNPWEFERPEVSYSIGFGGSVSSHMDDGEVARQLWQPTETIRAIA
                     YDTPIVGWRRTSVNTLRLWRARAEENLQLDRFNAGDHFGAVAEVVRAESISRVLYPAD
                     STEAGQELRLRQEFFFVSASLQDLLRRHMHENGDIRTLADKVAIQLNDTHPAIAVAEL
                     MRLLVDIHQLSWFEGWQLTVATLAYTNHTLLPEALESWPVSMMERLLPRHMQIIYLIN
                     AYHIDALRAQDIHDFALLRSVSLIEEDQGRRVRMGNLAFLGAHSINGVSALHTELMRQ
                     TVFRDLHALYPQRINNKTNGVSFRRWLFQVNPLLTQLLVDTLGSEVLDSPEAILCQLA
                     MHAGNGQLRQRFAAQRQQGKTALAQLIHERLGISVDTAAIFDVQVKRIHEYKRQLLNL
                     LHTVALYQAIRNDPTTDWAPRVKIFAGKAAASYHQAKLIIKLTNDLSRTINDDPTVRG
                     LLKVVFLPNYNVSLAEMIIPAADLSEQISTAGLEASGTSNMKFALNGALTIGTLDGAN
                     VEMSELIGCEHMFIFGMTASQVNLRKQAGDLNMSSTLMTSERLRLALEAIREGVFSPD
                     DPTRYAGLVDTLIADDRFMVCADFDDYWEAQRRVDQHWQNPDAWWTSAILNCSRMGWF
                     SSDRTIREYAADIWGVPVGKQ"
     gene            complement(15900..16220)
                     /locus_tag="VFF22_07865"
     CDS             complement(15900..16220)
                     /locus_tag="VFF22_07865"
                     /inference="COORDINATES: protein motif:HMM:NF022172.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HZX16766.1"
                     /translation="MKRDWNLIKELLLRIECLEPGQHFVKQALAQHCPHSVENHLRLM
                     HLAGLIEGALEQDAQGEPEFAAYSLTAQGRDFLGTIRESDSRKYARHSHCACFAQDDL
                     RQTA"
     gene            16415..16846
                     /locus_tag="VFF22_07870"
     CDS             16415..16846
                     /locus_tag="VFF22_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16767.1"
                     /translation="MDIRALLLLVGSSFAGSSVFADACHVSQSTTTSQVPAVVMESCY
                     EFTGMPADAINWSCSNESNQPLGSEKKKVVSCKSGSVAVCSAALTQESLANHRSTGDN
                     EQAADLNVPEDAKVITRYYSLEDSQQAKIDCENGGGTWKKL"
     gene            16843..17937
                     /locus_tag="VFF22_07875"
     CDS             16843..17937
                     /locus_tag="VFF22_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020293138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3182 family protein"
                     /protein_id="HZX16768.1"
                     /translation="MSTKAGVALLHVQQETAVHEQTVHRVLGQKIAALLGTAYLGSYD
                     PQAHDDCYLIPDETLVGEASLRTLGIRDVGDFFGGAINQPFMATKAITHTLIEQPTHV
                     PPGWSTRFSQQVSQAILRGYTVFDRDDAVRAGELLLQQGPVRLKPVRGKAGRGQEALS
                     DRQQLLTSIAAQDADEVATWGLVIEENLSEVITYSVGQVFVAGITASYYGTQRLTHDN
                     TGAEVYGGSDLVVVRGGYMTLSQLEMSPQARQAIEQACVYEQAAFECFGLLASRRNYD
                     VAQGVDALGQSRSGVLEQSWRIGGASAAEIFALEAFSMDPALHCVRASTYESYGENSP
                     PDDAVQLYSGSEPGLGILRKYAKIEPYSSS"
     gene            complement(18118..18579)
                     /locus_tag="VFF22_07880"
     CDS             complement(18118..18579)
                     /locus_tag="VFF22_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HZX16769.1"
                     /translation="MNHDNLDKTISVLNDLIETSKDGEAGFRTCAEDVKNVELKSLFT
                     KRAGECATAAEELQAMVLGFGGAPENDTSVAGDLHRRWVDLKSLVSGKDEEAILNECE
                     RGEDVAKKRYAEALKNELPLNVRELVQRQYEGVLRNHDQIKALRNSARMSS"
     gene            complement(18955..19293)
                     /locus_tag="VFF22_07885"
     CDS             complement(18955..19293)
                     /locus_tag="VFF22_07885"
                     /inference="COORDINATES: protein motif:HMM:NF023446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3509 domain-containing protein"
                     /protein_id="HZX16770.1"
                     /translation="MTSGTESEQALAEYSVKLTHRPDGNMLLTLRTGKKLALRKVLDG
                     ELVKSKESISSLIDDLLRDIKLASGDIAWQGKENRWIKKELPTFTGAPIQLTASKSLF
                     SRRKINSTTQ"
     gene            complement(19626..20018)
                     /locus_tag="VFF22_07890"
     CDS             complement(19626..20018)
                     /locus_tag="VFF22_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZX16771.1"
                     /translation="MDRFTGGCLCGNVRIVAMGRPYRVGLCHCLDCRKHHGALFHASA
                     IFPQEAVTIEGETHDYAGRFFCPRCGSSVYSRSADEIEVNLGSFDAPDQLTPTYELWT
                     IRRESWLPPFPLTKHYKRDRDSSHRVEE"
     gene            complement(20105..20731)
                     /locus_tag="VFF22_07895"
     CDS             complement(20105..20731)
                     /locus_tag="VFF22_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HZX16772.1"
                     /translation="MTQLFYLADLFGVAVFAITGALMAGRKSMDLFGVLVIAIITALG
                     GGTLRDVILDNHPVSWIRNDLYIVVASLAAVGTVVWVRLTQPIHEKGLLLADAFGLAV
                     FTVIGAEVAMQHNVPHSTAVIMGVMTGVAGGVMRDVICNEIPLIFQKEIYATACIAGA
                     LVFIGMRALDTPHWLDTGVAMLTVLLIRLAAIRWHISLPRFHLLDRHR"
     gene            complement(20739..22097)
                     /gene="gorA"
                     /locus_tag="VFF22_07900"
     CDS             complement(20739..22097)
                     /gene="gorA"
                     /locus_tag="VFF22_07900"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008012862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HZX16773.1"
                     /translation="MTYDFDLFVIGAGSGGVRAARFAAGYGARVAVAESRYLGGTCVN
                     VGCVPKKLLVYGAHFADDFEQAQGFGWSLGEARFDWPTLIANKNREIDRLNGIYRNLL
                     VNSGVTLLEGHARIVDDHCVELGGKRYSAKHILIATGGWPQIPEIPGHEHAIGSNEAF
                     FLEQLPKRVLVVGGGYIAVEFASIFNGLGAKTSLLYRGELFLRGFDKAVRLHLQEELN
                     KRGVDLQFNSDIARIDKQADGSLLATLKDGRTLEADCIFYATGRRPMLDNLGLENTHV
                     QLDERGFIEVDEQYQTSTPSILAIGDVIGRVQLTPVALAEGMAVARRLFKPEEYRKVD
                     YRMIPTAVFSLPNIGTVGLSEEQAKEEGYSVQVFESRFRPMKLTMTESQERTLMKLVV
                     DAATDRVLGCHMVGPDAGEIVQGLAVALKAGATKQLFDETIGVHPTAAEEFVTMRTPV
                     SS"
     gene            22180..22602
                     /locus_tag="VFF22_07905"
     CDS             22180..22602
                     /locus_tag="VFF22_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZX16774.1"
                     /translation="MRPTLTHVALHVPDLDACVAFYEQFCAMQVIHQRAGKGSRIVWM
                     AEPGKEHSFIFVIMPGGQDRQLAADDYSHFGFAMASRDEVDSIAERARLAGCLVWDPR
                     DEPYPVGYYCGLRDPAGNYVEFSYGQPLGPGAEHMPLA"
     gene            complement(22654..23493)
                     /gene="galU"
                     /locus_tag="VFF22_07910"
     CDS             complement(22654..23493)
                     /gene="galU"
                     /locus_tag="VFF22_07910"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HZX16775.1"
                     /translation="MIKKCLFPAAGYGTRFLPATKAMPKEMLPIVNKPLIQYGVEEAL
                     EAGLTEIAMITGRGKRALEDHFDISYELEHQIRDTDKEKYLVGIRRLIDECTFSYTRQ
                     VEMKGLGHAILCGRPLIGDEPFAVVLADDLCLNLDGDAVLTQMVKLYNQFRCSIVAIQ
                     EVPPEETHKYGVISGEMIREDIYRVNSMVEKPKPEDAPSNLAIIGRYILTPDIFDLIE
                     QTEPGKGGEIQITDALMKQAQNGCVMAYKFKGKRFDCGGAEGYIDATNFCFENLYKTG
                     KAY"
     gene            complement(23538..24899)
                     /locus_tag="VFF22_07915"
     CDS             complement(23538..24899)
                     /locus_tag="VFF22_07915"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HZX16776.1"
                     /translation="MRICVIGAGYVGLVSAACFAEMGNRVICVERDAQRVARLAHGQS
                     PIYEPGLEAMLQTHLSSGQLSFSQHLHDGIKQAEIIFIAVGTPSGEDGSADLSHVLAV
                     ADELGELLEQSCVVVNKSTVPVGTAGRVAQRINSRLSKRGKRFAVTLASNPEFLKEGS
                     AVDDFMRPDRVIVGCDEPLGHEVLRRLYAPFLRNHERLLCMGLRAAEFSKYAANAFLA
                     TKISFINEMASLCARLDVDIEDVRRGIGSDKRIGTHFIYAGCGYGGSCFPKDVRALIR
                     CAEQEGIEPGILRAVEARNALQKTLLFQALREHFSGYWQGRVVALWGLAFKPGTDDLR
                     EAPSLVLLDALLAVGVSVQACDPVATTALAERYGQAIASGQLRLSTSPYSVVEGADAL
                     VLVTEWKQFRQPDFVRIRGLMRMPVLFDGRNIYDAAQLAELGFLYRGIGRPAQGHCKA
                     SAA"
     gene            complement(25089..25175)
                     /locus_tag="VFF22_07920"
     tRNA            complement(25089..25175)
                     /locus_tag="VFF22_07920"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25139..25141),aa:Leu,seq:taa)
     gene            complement(25288..25361)
                     /locus_tag="VFF22_07925"
     tRNA            complement(25288..25361)
                     /locus_tag="VFF22_07925"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25327..25329),aa:Cys,seq:gca)
     gene            complement(25401..25476)
                     /locus_tag="VFF22_07930"
     tRNA            complement(25401..25476)
                     /locus_tag="VFF22_07930"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25441..25443),aa:Gly,seq:gcc)
     gene            complement(25544..26104)
                     /gene="pgsA"
                     /locus_tag="VFF22_07935"
     CDS             complement(25544..26104)
                     /gene="pgsA"
                     /locus_tag="VFF22_07935"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HZX16777.1"
                     /translation="MNIPNLLTVLRVLLIPVFILLFYLPFSWSYWAASAVFSVAAATD
                     WLDGYLARRWEQSTPFGAFLDPVADKLMVAVALVLLVAEHANLWLTLSASIIIGREIV
                     VSALREWMAELGARAHVAVSKLAKWKTAAQMVALVILLANPPTFTAWVVLGYVLLIIA
                     AVLTLWSMAQYLLAAWPHLRITSEKK"
     gene            complement(26138..27979)
                     /gene="uvrC"
                     /locus_tag="VFF22_07940"
     CDS             complement(26138..27979)
                     /gene="uvrC"
                     /locus_tag="VFF22_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZX16778.1"
                     /translation="MPTPDNSPAAFDSSAFLSTCSGRPGVYRMFDVDAKLLYVGKAKN
                     LKKRLASYFRQSGLAPKTAALVGRIAQIETTITANETEALLLEQTLIKEWRPPYNILL
                     RDDKSYPYVLLSDGDFPRLSIHRGAKKQKGRYFGPYPSAGAIRESLNLLQKTFLVRQC
                     EDSFYKNRTRPCLQYQIKRCKGPCVGLVEPAEYADDVRHSVMFLEGRSNALTDELSSG
                     MQAAAMALDFERAAGLRDQISLLRRVQDQQSMEGGTGDVDVVAAMVNPGGACVHLISV
                     RGGRVLGSKNFFPQVAIEEEGSDVLLAFLAQYYLSSQERDLPSELIVNAQHEDFPTLI
                     AAIDELRGRELSISYRVRGTRARWQQLAVTNAEQALAARLANRQHVAARFDALAEALD
                     MDEVPQRLECFDISHSSGEATVASCVVFGPEGPLKSDYRRYNIEGVTPGDDYAAMHQA
                     LTRRFSKIKDGEGKLPDILLVDGGKGQLAMAREVLEELSVPDLILLGVAKGTTRKPGL
                     ETLYLNDAAHEFTLPGNSPALHLIQQIRDESHRFAITGHRARRGKTRRTSTLEDVAGV
                     GPKRRRELLNHFGGLQELSRASIEEIAKAPGISKKLAELIYAALHSE"
     gene            complement(27966..28610)
                     /gene="uvrY"
                     /locus_tag="VFF22_07945"
     CDS             complement(27966..28610)
                     /gene="uvrY"
                     /locus_tag="VFF22_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HZX16779.1"
                     /translation="MIRVLVVDDHDLVRTGITRMLADIDGLQVVGQAESGEESLKKAR
                     ELKPDVVLMDIKMPGIGGLEATRKLIRSHPDLKVVAVTVCEEDPFPTRLLQAGAAGYL
                     TKGAALEEMVQAIRMVFSGQRYISPQIAQQLALKSFQPQSSNSPFDLLSEREIQIALM
                     IAGCLKVQAISDKLCLSPKTVNTYRYRIFEKLSITSDVELALLAVRHGMIDANT"
     gene            complement(28749..28838)
                     /locus_tag="VFF22_07950"
     tRNA            complement(28749..28838)
                     /locus_tag="VFF22_07950"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28802..28804),aa:Ser,seq:tga)
BASE COUNT         6330 a         8373 c         7945 g         6237 t
ORIGIN      
        1 ctgaaatcca ggctgtcatc aggaatcaca ggggcttctg gggttggggt actcatggct
       61 gcctccgaat tggttggctg accggtcgac cggggccgcc gatcactgat tcaaggtttg
      121 cgcgagcggc aggatgacca tcgggccatc cgccacactg cttgatgatc cgggccggtt
      181 tacggcgaga tcggatcact ggccgggaag gtttcttcca gggcgtgatc caccttggcc
      241 tcttgggcgc gtttcccgct tgatgtgttc ttttcaagct tgtccacggc cgctcgcagg
      301 tcagccacgc gctggcgcag atagaccagt tcggcgtgag ggtcagtgag catggccttg
      361 agcgaacagt ggccgctgat gccacggtgc accaacgcga ggccacccag tgccttgata
      421 aggcccgccg acccaccttg gtgaacacca ctaacaatgg ctgcggctcc cgtagcaatg
      481 gaaataccgc gttcaatacg ctggacgttg tgaatatttg atgacatatc gcctccgtca
      541 gactggttgc caggccactg caaacatcca gctgctgggc ccgcgcaggt tgcttaatgg
      601 gttagaaggc gggtctgcag gcaaaggttc cgcctatcgg tcgagcggtt cagagtcgta
      661 ggaggatgtc gcccgcttgc tcgatcacct gttcagctat tcgccgcctc taccgacgtc
      721 gcgcaactcg gtctgcgcca tcagcgcatc caccaccgcc atcaaggctt cgccgtgact
      781 gcgccccgcg tctcgttcct gctggtattg gtgcagttgc gcctgcgcac ttccgccgtc
      841 acggaccaga cgctgggcgt gctgcaccgc cgactcgtcg ccgggagcaa tgtgtgcctg
      901 cagttgctgc agcacgctgc cgagccactc ggcaaacggc agcgcacgct gcgttgttgg
      961 gtcgataaag gcaccgtgca agccataacg cttggctcgc cagcggttct ccagggtgat
     1021 cacgcgggtt atcgggtcat cgatccaggc agcgctgggc atatgcgtgg cgtaattgac
     1081 catggcccgg aacagcgcag cgatgcacaa ggcgtcctcc agttgcgggc aggcatcggc
     1141 gatacgcagt tcgagagtcg ggtagcgcac cgacgggcgg atgttccacc acacgtccgc
     1201 tgtggtgcgg atcgagccgg tgtccaggag catctgcacg tagcgctggt agtcagcgtc
     1261 attgtcaaaa tgatcgggaa tgcccatacg cggccactcg tcacagacgg cttggcgata
     1321 gctcatcagc ccagacggct tgccctccca aaacggtgaa gaggcgctca agcctagcaa
     1381 caggggcagc cagggcgtca cccgattcat gatgcggatc cggtccagct cggccgatac
     1441 gccgacatgc acatgcagcc ccgccagtac gctgcggctg gcgaccatct ggaagtcatc
     1501 gaagatctgc tgataccgcg ccacgccggt gggccgctgc tgtcgccact gcgccagtgg
     1561 ctgagttccg gctgccacca ggccaaagcc aaactccctg gccacctgcg ccagggtgcg
     1621 gcgtgcgctg ctcaggcatt cacgggcatc gtccaggtta tgcagaaccg gggtgaccac
     1681 ctcaaactgg gcggcgaaca tctctcgggt gaccaggtta cccagcgcca tctggcaggc
     1741 tgcagcgaag ccttcgacgg gctgacgggg catgcgccgg ctaagcagat cggtgaggaa
     1801 atattcttcc tcaatgccaa acgtcggtgg tgagcgatct gtcatgacgc cttccttaag
     1861 cagttcctgt ggttgatacg cagcggcctg catggttcaa cctgttcgct tacggcgctt
     1921 gcaggggaaa tttaggtgga attttttctg gcgcaaaacc ctcctaatta agaggcacag
     1981 agtcagccgc gaggttgttt tctggttgcc tgcccaaccg gccaggaggc ccagccaatg
     2041 tccattctca gccttgtcga cacctcctcc agcaaccacg ccccgcccct cactggcagc
     2101 ggttcgtggc aacagattta tcggcatctg ttagccgagc ctttggatgc acgcgaggcc
     2161 gcacgggact tcctcaatga gcgcctggag gaagccgccg acctgccctg tgagttgcca
     2221 gagcgccctg agcaattgca cgcctggtct ctgcgcgggt ccgctagtgt tactcaggcg
     2281 tatgcggcct acctggacaa gcggcgggcc ggcggcgcac gtcagtactt ccgccatcgc
     2341 gcccacgccc tgcattttct acgcgccgtg gcaccgacca aactcgtcga cggagcctgg
     2401 ttgtatggca ccttgccgca ctggcgggac gtgcgcgtgc agccgctggt gcgcacctac
     2461 ctggaagagt tgggcgatgg cgtacctcag cagaatcatg tgctgttgta tcgccggttg
     2521 ttgaccgctc agggctgcga cgaccttaac aacctggacg atgcgctcta cctgcagggt
     2581 gcaatccagt tggcgctcgg ccacttgggc agcgactacc tgccggaggt tatcggctac
     2641 aacctgggct atgaacagtt gcccttgcac ctgctgatca gcgcgttcga gctggaagag
     2701 ctggacatcg acccctatta ctttttgctg catgtgacca tcgacaacag cggcaccggc
     2761 catgcggcca aggcggtgcg tgccgtgctg gataacctgc cgctgttggg tgatgccaag
     2821 gcgttctatc gacgggtggc cttgggttat cggctcaacg atctgggcgt cagctcaacc
     2881 aggattatcg ccggcttcga cctggaacaa gagctgcacg caatgcttga acacaagcgc
     2941 ggtgtggctg ggcaggtgca ttccgattac tgccgcatcg aagggcggac gatcaaccaa
     3001 tggctggcct gccaagaagg cattggtggc ttcctcgacg cgctgcaacg ccggggttgg
     3061 attaaccgtc accaggaccc gatcctcagc cgcttctgga atctggtgcg cggcgatggg
     3121 gctgcgatgt tcggcgtatt cagcgcatac gagcagcagc tggtgcatga ctggatcgcc
     3181 ggtgactggc tcgatacgcc aactgccaaa catctggata cggcactctt tcgcaagaac
     3241 cgtcagcgtc aagacccgtt gttggagcca gcgagcgcgc cggccagcag cgagttggat
     3301 aacgaacagc aactactgcg caagcagctg cagcggatga gtgccgcaca acgcgaagcg
     3361 cggcttatcg aactgctggc cccggtttat cactccactt ctgccgggtt ggcggccacc
     3421 cgcatcttcg ctgcactggc ttactgaaac aggagagcat catgtatgaa gccctggcgc
     3481 cgctcgcctc ccctcaagcc ttggcgttgc tgcgcctggg cgctgcgctg agcgcccgtg
     3541 gttaccgctt tatcacgccc acaccggcga cccagcaacg ggtcaacctg cgcccaggca
     3601 atgagcaggc gcgcaacctg cgcgatatct tcggctggag ccggcccttt cgccgcgaga
     3661 cgctggatgc cgaactcttc gagctgatgc gccgggccga tgttctgcaa gcccagggtg
     3721 agcggtggct cagccgggtg cgctggtcga gcctggggga tcagctgttc gtccattcac
     3781 gcttcccgac cgacagcgcc gacgcggtat tcttcggccc agatacttac cgtttcgtgc
     3841 ggatggtgcg cgaccacttg caaagccaca gccatcccct acagcgcatc gtcgatatag
     3901 gctgcggcgc cggccctggg gcgttgacag ccgccatgct gcaaccgcag gccgaggtgc
     3961 tggcactcga catcaatgac caggccctcg cctttactgc tgtcaatgcg tgcctgggcg
     4021 gcgtcagcaa cctggtgacg cggcgcagtg acctattgcg tgatgtgacg gggaacttcg
     4081 atctgatcat cgccaacccg ccctacatgc tggatgcgca gcagcgcacc tatcggcatg
     4141 gcggtggcaa acatggcatt ggcttgtcac tgaagatatt cgacgcggcg cttgagcggc
     4201 tgacaccggg cggcaccctg ctgctctata ccggcgtagc catattcgac aacgaggatc
     4261 cgttcctgaa tgccatccgc ccatcgctgg ccgatatcaa ttggggctgg agctatgagg
     4321 aaatcgaccc ggatgtattt ggcgaggagt tggaaaaacc ggcttacgct tatgcagaac
     4381 gtatcgcttg tgtggcactg aaactcaccc gtccgttccc gaccgcaggt gccgatgaac
     4441 aggcccggca ggccgcgctg ctgcaaagct cttgattaag ctgcggagcg cggcgcactg
     4501 gcgccccgct acgagcgctc actgatgcag ttattttctg ccagccgacc gccttgcgca
     4561 ccgctctgcc ctgaaccccg tgctgtacat ggtctggtaa ggctgtgctt cgtcgtcgca
     4621 agccatggct taattcctcc ggtttaacgg caccacttga atgccgggct tatccgttaa
     4681 ccacgctgcc gccatttacg tgcagcatct gccccgtcat ataggaagcg tcattgctgg
     4741 ccaggtacac aaacgcaggg gccacttcag acggttgccc agggcgcccc attggcgtgt
     4801 ccgcgccaaa ggtggccact ttctcggcgc taaacgtaga gggaatcaga ggtgtccaga
     4861 tggggccagg ggcaacccca ttaacccgaa tgcctttggc cgccaggttg gtggagagtg
     4921 aacgggtaaa tgaggtgatg gcgcccttgg ttgacgaata atccaacagc atcgggttgc
     4981 ctttataggc ggtcaccgag ctggtgttga tgatgctggc gcctgcttgc atatgggcta
     5041 gcgcaagctt ggtgagttgg aacatggcga agatgttggt gcgaaaggtt ttctcccact
     5101 gttgctcatc catgtcttcc aggttgcttt cgggatgctg ttcaccggca ttgttgacca
     5161 gaatatccag gcgtccccat ttggcgatca ccgcgtctac gacacactta caaaatcctt
     5221 cttgcgctat atcaccagcc aaggcgatgg cctggccacc gtgcttggtg atctcgctca
     5281 gagtcttctg ggcatcctcg ttttcatcca gataaaccag cgcaacatgc gcgccctcca
     5341 gcgcaaaatg caccgccacg gcacggccga ttccgctgtc gccaccggtg atgatggcta
     5401 ccttcccggc aagcttattg gctgcccggt aatcggtgga tagatacgtc ggctggggat
     5461 gcatatcgtg ctcaaggccg ggttgccgat cctgatgttg cgcgggcaat gtttgtttat
     5521 cgtccattgt ggatccctct gatgaaggtg gccgttggcg atagccgaag cgtgcgcgac
     5581 accaggcctc ggctattcca gcggatcatg gattgggcaa aatgcacggc gctacacgac
     5641 ttccaagcgc ggcgcatgtg atgcggtttt gctcttgtag atgttctcga agcagaagtt
     5701 ggtggcctcg acatagcctt cggcagaacc gcaatcgaag cgttggccct tgaatttgta
     5761 ggccattacg cagccctgct gggcctgttt catcagcgca tcggtgatct gaatttcgcc
     5821 gtttttaccg ggaaccgtct gttcaatcag cgcaaaaatg tcaggggtca ggatgtagcg
     5881 gccaatgatt gccaggttgg acggtgcgtc ttcgggcttg ggtttttcca ccatccgttc
     5941 aacccggaaa atatcatcac gaatcgcctc gccagcgatg acgccgtagc gggagacctc
     6001 ttcaagggga acctcctgaa tcgccacgat ggaacagcga aactgctcat agagcttgac
     6061 catctgcgtc aacacgccat caccgtcgac gttcaggcac aggtcatcgg cgagcactac
     6121 ggcaaaggct tcgtcaccaa tcagcggacg cccacagagg atggcgtggc cgagcccttt
     6181 catttcaatt tggcgcgtat aggagaagct gcactcatca ataagacggc gaataccgct
     6241 caaggatttc tctttgctgg tattggagat ttcatgttcc agctcatagc tgatatcgaa
     6301 gtggtcttcc aacgaacgtt tgccgcgccc ggtgaccacg gcaatctcat tcagccccgc
     6361 ttcaagcgcc tcttctacgc catattgaat gagcggcgta tcaacaaccg gcagcatttc
     6421 cttgggcatc gacttggtag ctggcagaaa gcgtgttcca taacctgctg cggggaataa
     6481 gcatttcttg atcatggcta gtaccgagaa taatgggttg gtactaaaaa ctgacccgtc
     6541 agcttactgc caggttcaag gaatttttcc ttagctatgg gctgaaaata ccgccgagct
     6601 tggacagtcg acatcaactg cttacaactc gcaacaacgt gcgaaagttc acacttttag
     6661 tgcaagccat ttatgagtgc catataaatg atggcacaga aataatctgt acaaaaattg
     6721 tacacggggc gccatgcttt aacttgatta caactcaata atggcacttt actattgttt
     6781 agtaccgttt atccggaggc tatccggagc cgttgcgctt ggctgagcac gcgctatttt
     6841 ttcacttctc acatgtttct taaggagaag aaaaatgaaa gtggaggaat tgaccatcac
     6901 atccgagggg tatgcagtgc aggctacgca acggcccgat ggcagtatcc tattgaaatt
     6961 cagctcctct aatggaaaga ccatagagcg cgtcgccgga aaaataaccg atattggtga
     7021 agcagttcgc gctgtaaaat ttgacatagc ctatgaggct ggcaatagtt gcctgcatga
     7081 cgcagtaaaa tattgcagca gcactgctgg gctgccgacc tatatgcgta ccccactgtt
     7141 cagaacacat agctccagac tgtggagcac aagaaaattg cgagagtagt agacgcacat
     7201 accttaaaaa acaacctgaa tggaacttaa atatggatat cttaaatcgc gaaaggcgtt
     7261 tgatgagtaa gttctgtgaa tggtttgcac ggccggaaac caccaccttt attgcattta
     7321 tgtttatctt caatttaaag ctcctcttcc ctttggaggt ttctagcgaa ccgcaaaaac
     7381 ttttgtccat gcattacgca gaatcaaact gcgattttaa aaaccagctc aaagcgcagg
     7441 ccgcgtgtgt tggtaactgc cccgatcagg acgaatctgg atggaacctc aacaggctga
     7501 ctgtaacctg cgaacagggc gttatagagg gcgccaccgg ttggcatcag agggggatgg
     7561 acagcatggt gatacaagaa attaaaagaa tgccgatctt ctaaagcagt ggttgcggcg
     7621 cgaaaagttg ctacagcttt tctagcgccg ttccacatta cgccactttt gcagtaacgc
     7681 ctgcaactga gcctgctgat caagtagtac gccgtaatga accataagct ggtcacgaaa
     7741 cacgggcggc aggtcgccct gaagcagtgt ttcaaagcgt tgacagacgg ctctttcaac
     7801 gcgcaagcct tcattgagca agtcccggtc atccatcttc cctatacgta acttcagcca
     7861 ttgccaaccg cagtgcagca tgccaattac gctggcatgc cgacttgatc gtccaccata
     7921 gcttctgatc tgctgctgca gttgctcgcc cagtaaaggt aaggtcaacg caatgctggt
     7981 aaacagataa cgaagttctt cagcgtgcga tattttagca aaatcctgaa aaatcaatct
     8041 tgccgaataa cacgttacga ggcattgatt tagttgctcg atggtcttgt ctagtgttgg
     8101 catgccagtt ctctccaagc tgttatttca tataacgctt acttatgctc aggtatacac
     8161 ctgcggcagt actgcaaaat atttctaatc aggcactatt cctggccaat catttgttaa
     8221 ctgtgcagag tagttgcacg gttctgcttt gatgattagc agagcgtact agcaatgcgc
     8281 ttaagcacat cgttcgcttg cgcacagacg cactgcggca acgcactcat gctgatactt
     8341 catcgcagcg gccatacggc tacgcagcgg tctcatgggt taaatatctc agcaatcgga
     8401 ggtcacatgc acaacccaaa cgatacaaat agagaaggtg ttagcgcgct cggcaagggc
     8461 actggaccta tcgttgcagg ccctacagca cgaggcgaaa cgaaactagg ctcacccgat
     8521 gttgctcatg attttcataa tttcctggca gacatcgaag accttattaa ggatgtaacc
     8581 accctcactg gagaagatct ggcccaggcc aaggccaaat tgagctcacg tatcgacgct
     8641 gccaaagtgt ccgcagaagc tgtcggcgaa agcctttctc agcgggctaa gcagacggtt
     8701 gcatcaacca acgattacct gcatgagaag ccatggaccg ctctgtgcgc cggtgctgca
     8761 attgcctttc tattgggatt gttgaccgcg cgtcgcaact aaagctcacg aggctgaagc
     8821 atataagcct gatccagcca aagccggcgg ctggatcagg cttatctgag ctaccggcag
     8881 taacccgata attggcgggc tcactgaacc cggctttagc ctgcactggc gccgactcgc
     8941 cgataggtca ctgcattcaa ttgcgcaggt cggccgggca gcaggcggtc cgtttccttc
     9001 ctcaccacgg cataacactc gcagctcagg tgctcgagcc tggtgcggtc cagaacggtg
     9061 atgcgtccac ggtagtactc aatgatgcct tggcgttgca acttgccggc agcctctgtg
     9121 acgccttcac ggcgcacgcc aagcatgctg gcgatcagct cttgagtcat agccagctca
     9181 gtaccgggca ggcgatcaag ggataacaac aaccaacagc acaatcgctg ttcgatacag
     9241 tggtggcggt tgcatacagc agtttgcgac atttgcgtta tcagcgtttg ggtataccgc
     9301 aagaccaact gcagcagctc cccgtgacga tcgcactcat ccataaagcg ctgccctggc
     9361 aagcgatagg catggccact gttttgcaca accgcttgag tcgacatgct cccgccgccc
     9421 atcagcagcg cgaggccggc aaccccctca ttgccgacca gcgctgtctc agccgatgcg
     9481 ccactctcgg ttacacagag cagtgcaatg attgcatcgg ttgggaaata cacatgacgc
     9541 accgtatcgc ctgactcata gacgaccttg ccctgcggca actccaccca ttccatgcgg
     9601 ctgaacaagc gctcctggac gtcttttgaa agggatgcca aaagaagatt ttgatgggtg
     9661 ttttctggag ccactcgggc atcccagatt ttttttatcg gttgattcat ccatctaact
     9721 ccaaaggatc tgaggcgaaa gtcagttgca caggcagagt tattcgcaga cccatagcgg
     9781 tctttgcatc aggattcact tcaccactta tcaagaccgc agatccctga ttaagcgact
     9841 gctatacgga tgccgatagt tgctccggca attactccgc tcctggtaat cactcatagc
     9901 cacataaaaa ttcggctctg agggccataa aacgttcaac aaataattgc gcgtctctcc
     9961 cctcgcccgc ccccgcgaca acgctgtatg gcctgcccgt taagtgaacc aaatagcggg
    10021 aacatcttct gcttttgatg actcaaccca gagtggtgcc tgaaaaattg cgcccgaaat
    10081 gatcagacca agcttgagga aaaccagatg attaatcaga gaaaacccta cagcgccaat
    10141 cccacatccc ctagccagcc cagtgctggg agtcagcaaa agcctgattt taaagaggaa
    10201 caaacagcaa cccgcgcctg gcccacggtg cataaacagt tccgcatcgt tgctgccagc
    10261 cgccctgtac caggaaatgc cagcaaggaa acgtcgggct tagctgaata ggccgcagac
    10321 gaggttccag cagttcagga cgtaacacat gcagcacggg gcatccgcgc cccagccctg
    10381 tagcacgccg ctaaatcact cacgactcgg agtcgttcat gaccttcgac tggctgtcgt
    10441 atattcccac gccagatctg caagtcttgc tggcagcatt gcttgtcgca ctcgtcctgg
    10501 ctttcgcaac caaatggctg tttctgctac tgctgcgagt aacccactcg ctgaccttta
    10561 cccgcgagtt gctgctgcag gccaaggctc ctctgcgtct attgctgcca ttgctcggca
    10621 tacacacagt ctggaactcc agccccgatg agatcttctt tatcgacgcc gcacgccacc
    10681 tcacgtcgct ggccattatc ggcagcgtga cctggctcgg cttgcgcgct gtggatgcag
    10741 tgcagcagat catccttgtg catcatccgg tcaacgtcag tgacaacctg cgcgctcggc
    10801 gtatccagac tcagacccgc gtgctggtac gcacgctcag cgcactggtc atgatcttcg
    10861 gctttgcggc catgttgatg accttccctt ctgctcgtca gttcggggcc agtttgttgg
    10921 catcggcagg tttggccgga ctggccgtgg gttttgcggc caaacccgtg ctgggcaact
    10981 tgattgccgg cttgcaaata gcactcacac agcctatccg tctggatgat gtggtgatcg
    11041 tcgaaaatga gtgggggcgc atcgaagaaa tcaccggcac ctatgtcgtg gtgcgcatct
    11101 gggatgaccg ccggctgatc gtccccctgc aatggtttat cgaaaatccg ttccagaact
    11161 ggacacgcat gacctcatcg ctgatcggcg cggtaacgct gtgggtggac tattcgaccc
    11221 ctctggagcc gctacgccag gagttggcgc gactgtgtaa tgaggtgccc gagctctggg
    11281 atggccgtgt cagcgtgttg caagtgatcg acagcaacga acgcggtatt cagctgcgcg
    11341 tgctcgccag ctctggggat tcgtcacgta actgggacct gcgctgctat atccgggaaa
    11401 acctgatcaa ctttatcaat cgcgattacc cgcattgcct accgcagctg cgcggcgtgc
    11461 ttaaaccaga cgtcacgaaa gaacatcgcg acgaattacc ggctcagggc gatatcagcg
    11521 agccagaacg gcagcctccc gtttgacgaa tcagacctgg cgcaagcgct cgtagtaata
    11581 cggcagttgt agcagtgcat agcgtgatgc agcaacccgg acctcgttgc gctcgccggc
    11641 aaactttacc gtttcactca ccacgccttc atggccgttg accttcatgg cgcaggcgaa
    11701 acactgcacg ccgtccattt cgcctgccgc ttcggccagc ccagtattgg ccagaacgat
    11761 gtttgccttg ctgagcttca ttgcgccaat agccatttcc cgcgccacct cttcgctggt
    11821 taggccaaag gtctccatgg tctgcaggct cacgcccaga cacgcgtgct tggcctcggg
    11881 ggaatacacc acaaagccgg attcaagtac tgcgccagcc cccatcactt ccgccaccat
    11941 ggacgccatc agaccggctg tgcaggattc agccgtcgtc agcgacagct gatacttctc
    12001 tagaaaacgc accacctgtt caacactttg catgggctta cgaccttgtc tgtggctgaa
    12061 gttgttgtag atatgggaag cgtccacagc ttgcaaagtt caaggtttcg ccacggcctg
    12121 caacaggaaa taacacgaac tttcgccagg gcctccggtc agccagttat aaggcggccg
    12181 gtcgcgcaac agcaatgggg cggatgtttt ccgacgcacc agccagccga accgataaca
    12241 aacacgtgca gcgttctcgc tctcgtcgct ggcgaggcgg gacgaatgcc aacgatgggg
    12301 gcatacaaaa aaccatgagc gaagtctctg cagaaaagaa aaaacgctgg tgggtctggg
    12361 gcaagcgcct gctgactttg ctgttcttcg gcatggtctc agccatgctg tttatgctgg
    12421 tgaaaaacct cgactggcaa gacctcaaac aggcagtgca gtcgttcaag gcgagcacct
    12481 tgctgctgtg catcctgatc acaatgctca gctatagcct gttcagctgc tatgacttac
    12541 ttggccggat ctataccggg cacaaattac ccgccaagca gatcttgccg gtggcattcg
    12601 tctgctatgc cttcaacctc aatatcagcg cctggatcgg cagcatcgcc ctgcgttatc
    12661 gcctgtactc acgcttaggg ctggacctgg caaccatcac ccgcgtattc agcttcagcc
    12721 tggtgaccaa ctgggtgggt tacacgctga tcggcggcat cgtgttctcg ctgcgcttat
    12781 tggagctgcc ggcaaactgg tcaattggca ctgtgggact gcagctgatc ggcatcgcca
    12841 tgctggctgt atctatcacc tacctgctgg cctgtcgctt tgccaagcgg cgagcctggt
    12901 cctttcgcca acatgagctc accctgccct cgctgcgcct ggccttgctg caggtcgcca
    12961 tggcgacact caactggtca ctgtccagcc tggtggtatt tctgctgctg ccggacaagg
    13021 ttacctatgc gacggtgatc ggcattatgt tgatcagcag cattgccggc gtcatcaccc
    13081 atatcccggc ggggctcggc gtacttgagg cggtatttat cggcctgctg cagcatgaga
    13141 tcagcaaggg ctccatcctc gcggccttga tcggctaccg cgctatctat ctgctgttgc
    13201 cgctgctgct ggcctgcgtg gtgtatctcg ttctggagaa acgcgccaaa acgctacgcc
    13261 gccgcaccca cctacgtcag tcaggcaacg ccatctgcag tcataaatga ctgcagataa
    13321 gaaccaactc caggaggccc cagcatggcg acgaagcata tgttcttgat ccgctacagc
    13381 ctgaagtcac tgcttgccca cgggcacgcc ccagatgtcc gctgcatatt cgcggatggt
    13441 ccggtcagag gaaaaccagc ccattcgtga gcagttgagt atcgccgagg tccaccaggc
    13501 atccgggttt tgccaatgct ggtcgacccg ccgctgagcc tcccagtagt catcgaaatc
    13561 agcgcacacc ataaaacggt cgtcggcgat caacgtatca accagaccgg cataacgcgt
    13621 cgggtcatcg ggtgaaaaga caccttcacg gatcgcctct aaagccaatc gcagtcgctc
    13681 agaggtcatg agcgtactac tcatattcag atcgcccgct tgcttgcgca ggttcacctg
    13741 actggccgtc atgccgaaga taaacatgtg ctcgcagcca atcagctcgc tcatttccac
    13801 gttggcaccg tccagggtgc cgatggtcag cgcgccgttg agcgcgaact tcatattgct
    13861 ggtgcccgag gcttccaaac cggcagtgga aatctgctcg gaaaggtctg cagcagggat
    13921 gatcatctcg gccaggctga cattgtaatt gggcaggaat accaccttga gcaggccgcg
    13981 cacggtgggg tcatcattga tggtgcggct taggtcattg gtcaacttga tgatgagctt
    14041 ggcctggtga tagctggccg ccgctttgcc ggcgaatatc ttcacccgcg gcgcccagtc
    14101 ggtggtcggg tcgttgcgaa tcgcttggta caaggccacg gtatgcagca ggttaagcag
    14161 ttgacgtttg tattcgtgga tacgtttgac ctgcacatcg aagatcgccg cggtatcgac
    14221 gctaatcccc agacgctcgt gaatcagttg agccagcgcg gttttgcctt gttggcgctg
    14281 agcggcgaag cgctggcgca gctgcccatt gccagcatgc atggccaatt ggcaaagaat
    14341 ggcttccgga gaatcgagca cctcactgcc caaggtatca accagcaact gggtcagcag
    14401 cggattgacc tggaacaacc aacggcggaa tgacacgccg ttggttttgt tattgattcg
    14461 ttgcggatac agggcgtgca aatcacggaa cacggtctgg cgcataagct cggtgtgcag
    14521 cgccgatacg ccgttgatgc tatgcgcgcc aagaaacgcc aggttgccca tgcgcacccg
    14581 acgcccctgg tcttcctcga tcagcgacac cgagcgcagc agggcgaaat catggatgtc
    14641 ctgagcgcgc agtgcatcga tatggtaggc gttgatcagg tagatgatct gcatgtgccg
    14701 cggcagcaaa cgctccatca tcgatacggg ccaggactcc agcgcctccg ggagcaaggt
    14761 atggttggta taggcaagag tcgccaccgt cagttgccac ccctcgaacc aactcagttg
    14821 gtgaatatcc acgagaagac gcatcagctc ggcaactgca atcgcggggt gggtatcgtt
    14881 gagctggatg gctaccttgt ctgccagggt gcgaatatcg ccgttctcgt gcatatgccg
    14941 acgcagcaga tcctgcaacg aggcagagac gaaaaagaac tcctggcgca ggcgcagctc
    15001 ttgcccggct tcggtactgt ctgccggata gagcacccgc gagatactct ccgcgcgcac
    15061 cacttcggcc acggcgccaa agtggtcgcc ggcattgaag cgatccagtt gcaggttttc
    15121 ctcggcacgc gcccgccaca gccgtagcgt gttcactgac gtgcgccgcc aaccaactat
    15181 cggcgtgtcg taagcaatgg cgcggatggt ttccgttggc tgccagagct ggcgcgccac
    15241 ctcgccatcg tccatatggc tggagacact gccgccaaaa ccgatgctat agctcacctc
    15301 tgggcgttcg aactcccagg ggttgccaaa atccaaccag gtttccgttt gctcaagctg
    15361 ccaaccgtcg gcaatggctt ggcgaaacag gccatgctca taacggatgc cgtaaccatg
    15421 cgccgccaaa cccaaggtgg ccatgctttc cataaaacag gcggcaagtc ggccgaggcc
    15481 accgttaccc agtgcagcat cgggttcaaa ttgacggatt tgctcaaggt cgacattcag
    15541 ctcggccaat gcctggcgcg ccgtatcaag caagccaagg ttgctcaggt tgtcgaccag
    15601 caggcgaccg atcagaaatt ccagggatag gtagtagatg cgcttgggcg gatggcgttc
    15661 gacctctgcc gaggcctctt cccagcgatc cacgacatga tcgcgagcgg ccagagccac
    15721 cgcctcaaac cagtcgtgat caaaggcgct gcttgggtcc ctgcctaccg cgtatttgag
    15781 tttggcaaga atgctgtttt taaatgcctt tacgccatca gcgtcagagc tggtttcatc
    15841 cggcatgaag cgcctcgcag gttcagaggt aaaagctcag cggtaaaagc ggcgcgagat
    15901 caagccgttt gccgcaagtc atcttgagcg aagcaggcgc agtgactatg ccgcgcgtac
    15961 ttacgcgaat cgctttcgcg gatagtgccc aaaaaatcgc gcccctgcgc agtcaggcta
    16021 tacgcagcga actccggctc gccttgcgca tcctgctcta gagccccttc gataaggccg
    16081 gcgaggtgca taagtctcaa gtggttttcc actgagtgag ggcagtgttg agccaacgcc
    16141 tgtttgacga aatgctggcc cggttccagg cactcaatac gcagcagcaa ttccttgatc
    16201 agattccaat ctctcttcat gccctcgcct cactatttcg catcaggggg aatggattgc
    16261 gaccctagag ttgagtgccc ataggcatga cttagttcaa cctcttttgc cagctcattt
    16321 gcctgcgccg ccgcttatca gaaaccatga actttgcgcg acaggcccgc ttcagaacta
    16381 gagcgattca gccattccta gcaaggagat tgtcatggac atccgagcct tactgttgct
    16441 ggtcggcagt agctttgcag ggtcatcggt tttcgccgac gcctgccatg tcagccaaag
    16501 cacgaccact tcacaggtcc ctgctgtggt tatggaaagc tgctacgaat ttaccggtat
    16561 gcccgcagat gcgatcaatt ggtcatgcag caatgaaagc aaccaaccgc ttggaagtga
    16621 aaaaaagaag gtcgtcagct gcaaaagtgg ctccgtggcg gtctgttcgg cagcgttgac
    16681 tcaagagtca ttggccaacc accgctccac cggtgataac gagcaagcgg cggacctcaa
    16741 cgtgccggag gatgcaaaag tcatcacgcg ctattactcg ttagaagata gccagcaggc
    16801 caaaatcgat tgtgaaaatg gtggcggtac atggaagaag ctatgagcac taaagcgggc
    16861 gtagcgctgc tgcacgttca gcaagaaaca gcggtacacg aacaaactgt gcaccgcgtg
    16921 ctcggacaaa aaattgccgc actgctgggg actgcttatt tggggagcta tgacccgcaa
    16981 gcacacgacg actgctacct gatccctgac gaaacgctag tgggtgaagc ctccctgcgc
    17041 acgttgggca ttcgcgatgt gggcgatttt tttggcggcg ctatcaatca gccgtttatg
    17101 gcgaccaaag ccatcaccca cacgctgatt gagcaaccga cacatgtacc gccgggttgg
    17161 tccacgcgct tttcgcagca agtcagtcag gcaattctgc gcggctatac ggtgtttgac
    17221 cgggacgatg ctgtacgcgc aggtgagctc ttgctgcagc agggccctgt gcgcctgaag
    17281 cctgtgcgag gtaaggccgg tcgtggccag gaagcgttaa gcgaccgcca gcaattgctc
    17341 actagcatcg ccgcccaaga cgcggatgaa gtcgccacct ggggcctggt aatcgaggaa
    17401 aacctctcgg aggtcatcac ctacagcgtc ggccaagtgt ttgtcgccgg catcaccgcg
    17461 agctactacg gcactcaacg ccttacgcac gataatacgg gcgcggaggt atacggcggt
    17521 tccgatctgg tggtagtgcg tggcggctac atgacgctta gccaactgga gatgtccccc
    17581 caggcgcggc aggccattga gcaggcgtgc gtatatgaac aggccgcttt cgaatgcttt
    17641 ggccttttgg cctcacgacg caattacgat gtggcgcagg gcgtggatgc attaggccaa
    17701 tcacgctctg gggtactcga acagtcctgg cgcatcggag gggccagcgc agctgaaatc
    17761 tttgccctgg aagcattcag catggaccca gcgctgcact gcgtacgcgc ctccacctat
    17821 gaatcctatg gggaaaactc cccaccagat gatgcggttc agctttacag cggcagcgag
    17881 ccagggctcg gcattttgcg caaatacgcc aagatcgaac cctatagctc ctcatagaaa
    17941 ggcagcagaa tcctgtctta aacaagactc ccgctatgca accaaatcac gatgtgatcg
    18001 cccgcgatca agtcctggcg cttaggccat aagcagccta agcacgaagc ctttattgcc
    18061 gtacggcgcg cagctcaagc gccgtccatt acgtgacgac gcccgagccg caaggcatta
    18121 gctgctcatt ctagcgctgt tacgcagggc cttaatttga tcgtggttac gcagcacccc
    18181 ttcgtactgg cgctgcacta gttcacgaac gtttaacggc agttcatttt tcagagcttc
    18241 ggcatagcgc tttttagcga catcctcacc acgttcgcac tcattgagaa tggcctcctc
    18301 atctttacca gacaccaacg atttgaggtc tacccaacgg cggtgcaaat cgccagcaac
    18361 gctggtgtca ttctctggag cgccgccgaa cccgagcacc attgcttgca gctcttcagc
    18421 cgcagtagcg cactcgcccg cgcgcttggt aaataagctc ttcagctcca cattcttcac
    18481 atcttctgcg catgtacgga atccagcctc gccatccttg ctagtttcga tcagatcatt
    18541 gagtacgctg atagttttat ccaggttgtc atggttcata acagtttcct tgccgatttg
    18601 cggctatttg attgcagttc aggagcatcg gtacaaagcg atgtgggcgc tgccagtaga
    18661 gttcccccta ctctattcaa gtcattactt gaaatagcca gaggcactcc cctcccgttt
    18721 ataaagacga tggctgagta caccgcctag gtaggtgacc catgtatctg ctcaggtgtt
    18781 ccaccaatcg gatcggcggc ataaatagct ctgcgcccat cgcggccata tctcagcaac
    18841 aaaaatagcg atacacgttt aacgtatttt tgaaagaccc aagagatagc ggagattatg
    18901 tatccattca gacacataat ccctgcatta aatttatcta gaaatttatc cttgctactg
    18961 ggtagtgctg tttatttttc ttcttgaaaa caatgacttt gaagcagtta gctgaatggg
    19021 tgcaccggta aaagttggta actctttctt tatccagcgg ttttctttgc cttgccatgc
    19081 aatatcaccg ctggctaact ttatgtctct aagcaagtca tcgatcaaag agctaatact
    19141 ttctttagat ttgacgagtt caccatccaa aactttgcgc aatgccagtt tcttacccgt
    19201 gcgcagtgtt agcagcatat tcccatccgg gcgatgcgta agctttacgg aatactctgc
    19261 tagagcttgt tcactttcag tacctgatgt catgcctgac cctccatgtt gagtcaggct
    19321 cttcgcatga aatatgccag tcttgagatt ttgtatctaa ctgtttttaa tcaactattt
    19381 ttagccggca agctcagccc ggattgcaaa atgcatgatg ccctcgcaag gtatattgca
    19441 atttgcaagg taataaagtg ccatcaataa cgcttatgcg tactcaacca tcagagaagt
    19501 taaggttatc gtggagcgag ttttttactg gcaatggcgt taattaattt tccgagcaag
    19561 ctgtaagttt atgtgaagct gttttcaggc cacatacagt tcaaccctga tacgcgcctc
    19621 gcgtactact cctccacacg gtgagagcta tcgcggtcgc gcttgtagtg tttcgtgagt
    19681 ggaaatggag gcagccacga ctcacgacga atcgtccaaa gttcgtatgt tggcgttagt
    19741 tgatcagggg catcaaaaga gcccaggttt acttcgattt catctgcgct acgggagtaa
    19801 acggacgagc cgcaacgggg acagaaaaat cgcccggcgt agtcatgcgt ttcgccctcg
    19861 atagtgactg cctcctgcgg aaaaatcgca gaagcgtgga acagcgctcc gtggtgtttg
    19921 cggcaatcaa gacaatgaca aaggccgacc cggtacggac gccccatagc aacaatacgg
    19981 acattgccgc aaagacaacc accagtgaac cgatccatat tgcgtctcct ctagcgtcaa
    20041 gatgatgccg ggatgcccgt tatggcgctc ttcgcctccc cactcctgca gagaccaggt
    20101 gcagtcagcg atgcctatcc aggagatgaa agcggggcaa cgaaatatgc cagcgaatgg
    20161 cggccaggcg aatcagcagc acggtgagca tggccacccc cgtatccagc caatggggcg
    20221 tgtccaatgc gcgcatgccg ataaacacca gtgcacccgc tatgcaggca gtggcgtaaa
    20281 tttccttctg gaaaatcagc gggatctcgt tgcagatcac atcgcgcatc acgccgccgg
    20341 ctacaccggt catcaccccc atgatcaccg ccgtgctgtg cggcacgttg tgctgcatgg
    20401 cgacttcagc accgatcacg gtaaacaccg ccaagccaaa ggcatccgct aatagcaggc
    20461 ctttttcgtg aatcggctgg gtcagccgca cccagaccac ggtgccaacc gccgccagcg
    20521 aggcgaccac gatatacagg tcgttgcgaa tccagctgac cggatggtta tccagaatca
    20581 cgtcacgcaa ggtgccgcca cccagtgcag tgatgatggc aataaccagc acgccgaaca
    20641 ggtccatgga cttgcgccca gccatcagcg cgccagtaat ggcgaagacc gcgacgccga
    20701 acaggtcggc cagatagaac aattgcgtca tgctgacctc aggacgacac gggggtacgc
    20761 atggtgacga actcttcggc ggctgttggg tgcacgccga tggtttcatc gaataactgc
    20821 ttggtcgcgc ctgctttaag cgccacggcc agcccttgga caatttcacc ggcgtccggg
    20881 ccgaccatat ggcagccgag cacgcggtct gtggcagcgt ctaccaccag tttcatcagg
    20941 gtgcgttcct ggctttcggt catggtcagt ttcatcgggc ggaagcggct ttcgaaaacc
    21001 tggacgctgt agccctcctc cttcgcctgc tcttcgctga gccccaccgt gccgatattc
    21061 ggcaggctga ataccgccgt tgggatcatc cggtaatcaa ccttgcggta ttcctctggc
    21121 ttgaacaggc gccttgccac tgccatgcct tcggccagcg ctaccggtgt gagctgcaca
    21181 cggccgatca catcaccaat ggcgagaatc gagggtgtgc tggtctggta ttgctcgtcg
    21241 acctcgataa aaccgcgctc atccagctgt acatgggtgt tttccagccc caggttatcc
    21301 agcatcggcc gacgccctgt ggcgtagaag atacagtctg cttccagcgt tctaccgtcc
    21361 ttgagtgtgg ccagtaggct gccgtcggct tgtttgtcga tacgggcgat atcgctgttg
    21421 aactgcagat cgacaccgcg cttgtttaac tcttcctgca ggtgcagacg cacagcttta
    21481 tcgaaaccac gcaggaacag ctcgccgcga tagagcaacg aggtttttgc gccgaggccg
    21541 ttgaagatcg aggcaaactc cacggcgata taaccaccgc ccactaccag tacgcgcttg
    21601 ggcagctgct caagaaagaa ggcttcattc gagccgatgg catgttcatg ccctgggatt
    21661 tcaggaatct gcggccagcc gccagtggca atcaggatat gtttggcgct gtagcgttta
    21721 ccgcccagct cgacacagtg gtcatcgaca atccgcgcgt ggccttccag caaggtcaca
    21781 ccgctgttaa ccagcaggtt gcggtagatg ccattaagtc ggtcgatttc gcgatttttg
    21841 ttggcaatca gggtcggcca gtcgaagcga gcctcgccca gcgaccagcc aaacccctgg
    21901 gcctgttcga agtcatcggc gaaatgcgca ccatagacca gcagcttttt cggtacgcag
    21961 ccgacgttga cgcaggtacc gcccaggtaa cggctttcgg caaccgccac gcgcgcacca
    22021 tagccggcgg caaatcgcgc agcccgcaca ccgccggaac cggcaccaat cacaaacagg
    22081 tcaaagtcgt aggtcatttc agcctccaga acaggctgcc agcatacccc aaggcgcgtc
    22141 agtgcctaaa cttgaagcat gccgaatagg agatcagtga tgcgccctac ccttacccat
    22201 gtggcgttac atgtgcccga tctggatgca tgcgtagcct tttatgagca gttctgcgca
    22261 atgcaggtga ttcaccagcg cgcaggcaaa ggctcaagga ttgtctggat ggccgagccg
    22321 ggcaaggagc acagctttat ctttgtgatc atgcccggtg gtcaggaccg ccaactggcg
    22381 gcggatgatt acagccactt tggctttgcc atggccagtc gggatgaggt cgacagcatt
    22441 gccgaacgag cccgactggc cggatgtctg gtctgggatc cgcgtgatga gccctaccct
    22501 gttggctact actgcggcct gcgtgacccg gcgggtaact atgtggaatt cagttatggc
    22561 cagccgctgg ggcctggcgc tgagcatatg ccactggcat gaatgtaaac agccacccga
    22621 aggtggctgt ttgcggtcgc ggcgaagact gaatcagtaa gccttgccgg tcttgtagag
    22681 gttttcgaag cagaagttgg tggcatcgat atagccttct gcaccgccgc aatcaaaacg
    22741 cttgcccttg aatttatagg ccatcacaca gccgttctgc gcctgtttca tcagtgcgtc
    22801 ggtgatctgg atttccccgc ccttgccggg ctcggtttgt tcgatcaggt cgaagatatc
    22861 cggggtgagg atatagcggc cgatgatcgc caggttcgac ggtgcatctt ccggcttggg
    22921 cttttcgacc atgctgttaa cccggtagat gtcctcacga atcatctcgc cggagatcac
    22981 gccatatttg tgggtttctt ctggcggcac ttcctgaatg gccacgatcg agcagcggaa
    23041 ctggttgtac agcttgacca tctgcgtcag caccgcgtcg ccgtccagat taaggcacag
    23101 gtcatcggcc agcaccacgg cgaaaggctc atcgccaatc agcggacggc cacagagaat
    23161 cgcgtggcct aaacctttca tctcaacctg gcgggtgtag gaaaaggtgc attcgtcgat
    23221 caggcgacgg atgccaacta ggtatttttc cttgtcggtg tcacggatct ggtgctcaag
    23281 ctcgtagctg atgtcgaagt gatcttccag cgcgcgcttg ccgcggccag tgatcatggc
    23341 aatttccgtc aagccggctt ccagcgcctc ttcaacgccg tactgaatca gcggcttgtt
    23401 cacgatcggc agcatttcct tgggcatggc cttggtggct ggcagaaagc gggtgccgta
    23461 accggccgct gggaacaagc atttcttgat catgggaaac cttgggtaga tgaggtgaaa
    23521 atcgcgtggg cagtttatca ggctgcactg gccttacaat gcccctgcgc gggccggcca
    23581 atacctcggt agagaaaacc cagctcagcc aactgcgcgg catcatagat attgcgccca
    23641 tcaaacagca cgggcatgcg catcaaaccg cgaatgcgga caaagtcagg ctggcggaac
    23701 tgcttccatt cagtgaccaa caccagcgca tccgcgcctt caacaacgct gtaaggcgat
    23761 gtactcaaac gcagttgacc gctagcaatc gcctggccgt agcgttctgc cagagccgtt
    23821 gtggcgaccg ggtcgcacgc ctgaacgctg acaccgaccg caagtaaggc atccagcaga
    23881 accaggctcg gtgcttcgcg caaatcatcg gtgccgggtt tgaaagccaa gccccacaac
    23941 gccactacac gtccttgcca ataaccggaa aagtgctcgc gcagcgcttg gaacaacagg
    24001 gtcttctgta gcgcattacg ggcttcgact gcccgcagga tgccgggttc aatgccttcc
    24061 tgctctgcgc agcgaatcag cgcccgcaca tccttgggga aacacgagcc accataaccg
    24121 caaccggcat aaataaagtg agtgccaatg cgcttgtcgc tgccgatgcc acggcgcaca
    24181 tcctcgatgt cgacatccag gcgggcgcag agactggcca tttcgttgat aaaggaaatc
    24241 ttggtggcca aaaaggcgtt ggcggcatat ttgctgaact ctgccgcccg caggcccatg
    24301 cataacaagc gctcgtgatt gcgtagaaac ggcgcataca aacggcgcaa tacctcatgg
    24361 cccagcggct catcgcagcc aacaatcacc cggtcaggcc gcataaagtc gtcaaccgcc
    24421 gagccttcct tgagaaattc cgggttgctc gccagcgtca ccgcaaagcg cttgccgcgt
    24481 ttgctcaatc gactgttgat gcgctgggca acacgcccgg ccgtgcccac gggcaccgtt
    24541 gatttattca ccaccacgca gctctgctca agcagctcgc ccaactcatc ggccacggcc
    24601 aggacatgcg acaaatccgc cgagccgtcc tccccgctcg gcgtgccgac ggcgataaaa
    24661 atgatttcgg cctgcttgat gccgtcatgc aggtgctggc taaaactcaa ctggccgctg
    24721 ctcaggtgag tctgcagcat ggcctccagc ccaggctcgt aaatcggcga ctggccatgg
    24781 gcaagccgag ccacacgttg cgcatcgcgc tcaacgcaga tcaccctatt gcccatttcg
    24841 gcaaaacagg ctgccgatac cagccccacg tatcctgcgc caatcacaca aatccgcatc
    24901 gcctgcactc ctcactgagt taccgcgatt gcagctaaag cagatggcag gcgtatgaca
    24961 taagtacgac actcgggtga cagcacttga taagccgtgc gctcaaattt caggcacaaa
    25021 aaaaccggct acgaaggccg gttctagtgg atttcaggtt catacaggaa catctgaaat
    25081 catgtacatg gtgcccgaag ccggaatcga accggcacga ggttaccctc gagggatttt
    25141 aagtcccttg cgtctaccga tttcgccatt cgggcggtag cgctacgttg taaggcttgg
    25201 actatataca gcccttcgat gcctcgcaag gtttcaaact aaaatgaaaa agcctcgtaa
    25261 atcagtgatc tacgaggctt ttctaattgg aggctgaggt cggaatcgaa ccggcgttca
    25321 cggatttgca atccggtgca taaccactct gctactcagc cttgaaacta aacggaccgt
    25381 gtaaaacggc ccgttgaaaa tggagcggga aacgagactc gaactcgcga ccccgacctt
    25441 ggcaaggtcg tgctctacca actgagctat tcccgcttgt cgtgctgacg gccgccattc
    25501 tatagcttca ggacggccag tcaacccctt gattcaaaaa agcttatttc ttttccgagg
    25561 tgattcgcag gtgtggccat gcagccaaca ggtactgcgc catcgaccac agggtgagta
    25621 cggcagcaat gatcaacagg acgtaaccca gtacaaccca ggcagtaaat gttggcggat
    25681 tggccagcaa aatcaccaaa gcgaccatct gagcggctgt tttccatttg gccagttttg
    25741 aaaccgcgac atgcgccctg gcgcccaatt cagccatcca ctcacgcagt gcagaaacaa
    25801 caatttcacg gccgatgatg attgatgctg acagggtcaa ccagaggttg gcgtgttcgg
    25861 ccaccagtaa taccagggcc accgccacca tgagtttgtc agcaacggga tcaagaaatg
    25921 caccaaaagg tgtgctctgc tcccagcgac gggccaaata accgtccagc caatcggttg
    25981 cagccgcgac tgaaaacact gcactcgccg cccaatagct ccaactgaag ggaaggtaaa
    26041 acagcagaat aaacaccggt atcagtaata cgcggagcac agtaagcagg ttggggatat
    26101 tcatcgacac aacttggcgg taaagagaat cggcattcta ctcactgtgc agggccgcat
    26161 aaatcaactc agcgagtttt ttgctgattc caggtgcttt ggcaatttct tcgatgctgg
    26221 cacgtgataa ctcttgcagc ccaccaaaat gattaagcag ctcacgccgg cgcttaggcc
    26281 ctacgcccgc gacgtcctca agtgttgagg tgcgacgggt tttaccgcga cgggcacgat
    26341 gtccggtgat agcaaaacgg tgtgattcat cgcggatctg ctgtatcaga tgcagcgctg
    26401 gtgaattccc cggcaaggtg aactcatgtg cggcgtcatt caggtagagg gtttccaaac
    26461 ccggtttgcg cgtggtgcct ttggcgacgc cgagcagaat caggtctggc accgacagct
    26521 cttcgagcac ttcacgggcc atggccagct gccctttgcc accatcgacc aagaggatgt
    26581 ccggcagctt gccttcgcca tccttgatct tgctgaagcg ccgagtgagc gcttgatgca
    26641 tggcggcata atcgtcaccc ggcgtcacgc cctcaatgtt gtagcggcgg taatcggatt
    26701 tcagcggccc ctcggggccg aataccacac aagaggccac ggtcgcctcg ccgctggaat
    26761 gactgatgtc aaagcactcc aggcgttgcg gcacttcatc catatccagc gcttcggcca
    26821 gggcgtcaaa gcgcgcggca acgtgctgtc gattggctag gcgtgctgca agggcctgct
    26881 ccgcattggt caccgccagc tgctgccagc gcgcgcgagt accgcgaacg cggtagctga
    26941 tgctcagctc gcggccgcgt agctcatcga tggcggcgat caatgtgggg aagtcttcgt
    27001 gctgcgcatt gacgataagc tcgctcggca gatcgcgttc ctgactgctc aagtaatact
    27061 gcgcgaggaa ggcaagcagt acgtcactgc cctcctcctc aatcgccacc tgcgggaaaa
    27121 agtttttact gccgagcact cggccaccac gcacactgat caaatgcacg catgcgccac
    27181 cgggattgac catggccgcg accacgtcca catcaccggt tccaccttcc atgctctgct
    27241 ggtcttgaac ccggcgcagc agcgatatct ggtcgcgtaa tccggcagcc cgctcaaagt
    27301 cgagagccat ggcggcggcc tgcatgccgc tggacagctc atcggtcagg gcattgctgc
    27361 gcccctcaag aaacatcacc gaatggcgga cgtcatcagc atattcagcg ggctctacca
    27421 ggccgacaca ggggcctttg cagcgtttaa tctggtactg cagacagggc cttgtgcgat
    27481 ttttgtagaa actgtcctcg cactggcgca ccagaaaagt tttttgcagc aggttaaggc
    27541 tttcgcgaat ggcccctgcg ctgggatacg ggccgaagta cctgcctttc tgctttttcg
    27601 ccccgcggtg aatgctcaga cgcgggaagt cgccgtcgga aagcagcaca tagggatagg
    27661 atttatcgtc gcgcagcaga atgttgtacg gcggccgcca ttctttgatc agcgtctgct
    27721 caagcagcag ggcttcggtt tcgttggcgg tgatggtggt ttcgatctgg gcgattcggc
    27781 cgaccaacgc ggcggtcttt ggcgctaagc cgctctggcg gaagtagcta gccaggcgct
    27841 ttttcaggtt tttggctttg ccgacataca gcagtttagc gtccacatcg aacatgcggt
    27901 acacgccagg acgaccgctg caggtggaga ggaatgcgct ggagtcgaaa gcggcaggac
    27961 tgttatcagg tgttggcatc aatcatgccg tggcgtacgg ccagcagggc cagttcgaca
    28021 tcactggtaa tcgagagctt ttcaaagatg cgatagcggt aggtattcac ggttttcggc
    28081 gacaggcaaa gcttatcgga aatggcctga acctttaggc agcctgcaat catcagggcg
    28141 atttgaattt cgcgctcgga cagtaagtcg aacggtgagt tactgctctg tggctgaaat
    28201 gacttgagtg ccagctgctg ggcaatctgc ggactgatat agcgttgccc gctgaatacc
    28261 atgcgaatcg cctgaaccat ttcttccagc gcagcgcctt tagtgagata gccagcggca
    28321 ccagcttgca ggaggcgcgt ggggaaaggg tcttcctcac atacggttac ggcaacaact
    28381 ttaagatcag gatggctgcg aatcagcttg cgggtggctt caagaccgcc gatacctggc
    28441 attttgatgt ccatcagaac aacgtcaggt ttcagctcgc gggctttttt cagggactct
    28501 tcgcctgatt cggcttggcc gaccacctgt aaaccgtcga tatcggccag catacgagta
    28561 atgccggtgc gtaccaggtc gtggtcatca acaacgagaa ctctgatcaa gcgtcacccc
    28621 ggtcgacatc aactgcgttg agcagttgct gggttgaaag tctggtgcct gtaggcaacc
    28681 agcttggcgc ataaccgtca aaaggcaagc gccaataagt gagcgctaaa tgacggccag
    28741 agaaaatatg gcggaagcgg tgagattcga actcacggaa gagtttcccc ttcgacggtt
    28801 ttcaagaccg ttgcattcaa ccgctctgcc acgcttccgg cgtttgcggg cggcaatctt
    28861 acccgaacgc aacacgctgt caaac
//
LOCUS       DASRUM010000116         6511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1237914, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000116 DASRUM010000000
VERSION     DASRUM010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237914"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..2649
                     /locus_tag="VFF22_07955"
     CDS             232..2649
                     /locus_tag="VFF22_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HZX16780.1"
                     /translation="MMRLLRFFCWSGLAAFCALVLSFSGAFLYLSPSLPSVESLRSIQ
                     LQIPLRVYSSDAKLIAEFGEMRRSPIAFADIPPDFISALLAAEDDNFANHYGVDLTSL
                     MRAATQILKSGQIQTGGSTITMQVAKNFFLTSERSFSRKINEILLALQIERELSKNEI
                     LELYVNKIYLGNRAYGIEAAAQVYYGKSIRDVSLSQMAMIAGLPKAPSRFNPLVNPTR
                     ALERRDWILGRMHSLGRIDQQRYEEALAEPINVSYHVPTPELVAPYIAEMARAEMVGR
                     YGSEAYTEGFNVTTTVPSDLQMAANMAVREGLITYDQRHGYRGPESRLPGMTKENWQI
                     ELSKQTSLGGLEPAIVTGVEKSGIMVLLRNGEEHAVAWDSMKWARPFLNTNSMGPRPQ
                     QPADVSQIGDLIRVQRQEDGSLRFVQLPVAQSALVSLDPNNGAIRALVGGFSFEQSNY
                     NRAAQAKRQPGSSFKPFVYSAALDKGYTAATLVNDAPIVFADDALSQVWRPKNDNNTF
                     LGPIRLREALYRSRNLVSIRLLQDLGIDSTLSYIERFGFEKKDLPPNLSLALGTASLT
                     PMEIAEGWSVFASGGYKTEPYLIERIEDRTGKQLFNANPAQTPTGIAQRAEQSSAVQL
                     SVGDQPLPPEPVLAEQIIDERTAYIMTSMLQDVIKRGTGRRAMALGRDDLAGKTGTTN
                     ESKDSWFSGYNADYITTVWAGFDQPESLGRNEYGGTVALPIWMSYMAAALKDKPSHLL
                     AEPEGLLTLRIDPHSGRAATPDTPEAYFELFKSEDSPPPMGEFEPGTYIPGSPLPADE
                     SAPIDLF"
     gene            complement(2745..4013)
                     /locus_tag="VFF22_07960"
     CDS             complement(2745..4013)
                     /locus_tag="VFF22_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HZX16781.1"
                     /translation="MSDLKTAALEYHAHPRPGKLSVELTKPTATARDLSLAYSPGVAE
                     PVREIARDPELAYRYTGKGNLVAVISDGTAILGLGDLGPLASKPVMEGKGVLFKRFAG
                     IDVFDIEVESESPQAFIDTVRRISITFGGINLEDIKAPECFEIERTLIEQCDIPVFHD
                     DQHGTAIVTAAGMLNALEIAGKTLPDAKIVCLGAGAAAISCMKLLVSMGAKVENIFMI
                     DRKGVIHAGRDDLNQYKAVFASETTKRTLADALDGADVFVGLSGANLLAPEDLKRMAP
                     SPIVFACSNPDPEIKPELAHEVRNDVIMATGRSDYPNQVNNVLGFPFIFRGALDVRAT
                     RINEEMKIAAALALRDLAKLPVPQEVCDAYGGIALSFGREYIIPKPMDPRLITVVCDA
                     VAKAAIESGVATLPYPKHYPLQSVDDVFNG"
     gene            complement(4116..5549)
                     /locus_tag="VFF22_07965"
     CDS             complement(4116..5549)
                     /locus_tag="VFF22_07965"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZX16782.1"
                     /translation="MRVLTLFVLLLQLAGCAVNPATGRSNFVMMSEQQELDLGRRYNQ
                     EILKQNPRYADEKLQAYVQQVGERVAKHSHRNQLAYQFTVVDSPDINAFALPGGYIYI
                     HRGLLAYLSSEAELAAVLGHEVGHVTARHSVQQQSQSTAWGLLGQAVAIGTGVGAAAD
                     VTGVLGSAFVRGYGRDMELEADGLGAQYLARSGYDPQAMIEVVKVLKNQEDFARDQAA
                     ARGEAQPAGGYHGLFDTHPDNDRRLQQVLGPARALATGQQEVNREAFLKRLEGLPFGD
                     SAETGVRRGQRFYHADLNFTLAFPQGWSLLNRPDALIGHTADQQAFIAMTLEDNPQKL
                     APAELLRQRVGGQRLVAGVELQQAGLKGYSAVIPGNAAKRVAVIQHGPRVYLFVGAVR
                     GRASLESQDEQFLSVIKSFRPMTSAEFKEAQPLRLHMLKAKPGQTIEALAKGSKLPGD
                     ALKTLRLLNNLYPSGQPRSGDWLKVVR"
     gene            complement(5552..6352)
                     /locus_tag="VFF22_07970"
     CDS             complement(5552..6352)
                     /locus_tag="VFF22_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HZX16783.1"
                     /translation="MGCTALLKKASLVGAFFIFGVYSAAALAFCPAPGNLPSVKVQRV
                     VDGDTLRLVDGRNVRLIGLNTPEMGRQGRSAEPFAEAARKRLQALVAASDERVSLQYG
                     KQAKDHYGRTLAHAYDNRGRNLEAQLLAEGLGYLVAVAPNLALVPCQQAAERSARQAR
                     AGLWQRSPVQAAEQLSSGGFALLQGQVERVERNGGGLWLEMGDSLVLHIAPRALDNFD
                     LRAVQALEGRKVEARGWVIDRSKRGGLRAGQARWMLPLTDKAMLEVLP"
     gene            complement(6379..>6511)
                     /gene="rpmE"
                     /locus_tag="VFF22_07975"
     CDS             complement(6379..>6511)
                     /gene="rpmE"
                     /locus_tag="VFF22_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HZX16784.1"
                     /translation="AKPLSLDVCNECHPFYTGKQKMLDTGGRVDRFKQRFGMFGAKQ"
BASE COUNT         1377 a         2042 c         1840 g         1252 t
ORIGIN      
        1 atatcgatcc cgagtagcgt attcgctttc ttattgaaga gccctagcac gaccgatttc
       61 ctattggcat ccgcgactta cggactattt atgttttttc acattaaata acagtcttga
      121 cagaagcgca aatggctacc cagcaaaaaa accgcttata atgtgatcag tttttctctt
      181 ttaaccttgt gttccgctta actcgttctt ttatctggaa atccaaaaat cttgatgcgt
      241 cttttgcgtt ttttctgctg gtctggcctt gccgcttttt gtgctctggt gctcagtttc
      301 agcggggcct tcctctatct tagccctagc ctcccatccg tcgaatcctt gcgcagcatt
      361 cagctgcaga ttcccctacg cgtttacagc agtgacgcca agctgattgc cgaattcggt
      421 gaaatgcgcc gttcgcccat cgccttcgca gatatccccc cggacttcat cagcgccttg
      481 ctggcagctg aggacgacaa ctttgccaac cattatggcg tcgaccttac gagcctgatg
      541 cgtgctgcaa cgcaaatctt gaaaagcgga cagattcaaa ccggcggaag caccattacc
      601 atgcaggtgg cgaagaactt ctttctcacc agcgaaagaa gtttctcgcg caagatcaac
      661 gagatacttc ttgctctgca aattgaacgg gagctcagta agaacgaaat tcttgaactt
      721 tatgtgaaca agatttacct gggtaatcgc gcatacggca tcgaagcagc tgcgcaggtg
      781 tattacggta aatccattcg cgacgtcagc ctttcgcaaa tggcaatgat tgcaggcctg
      841 ccaaaagcac cttcgcgctt caacccgctg gtcaacccaa cacgcgccct tgagcgccgc
      901 gactggattc tcgggcgtat gcacagcctt gggcgcatcg accagcagcg ctatgaagaa
      961 gcactggccg agccgattaa tgtcagttac cacgtcccaa cgcctgagct cgtcgcaccc
     1021 tatattgccg aaatggcacg cgccgagatg gtcgggcgct atggcagcga ggcgtacact
     1081 gagggcttta acgttaccac cacggtgccc agcgacctgc agatggccgc caacatggct
     1141 gtacgtgagg gcctgattac ttacgatcag cgccacggct atcgcggccc ggaaagccgc
     1201 ctcccgggca tgaccaagga aaactggcag attgagctga gcaagcaaac ctctctcggc
     1261 ggcctggaac cggccattgt cacgggcgtg gagaaaagcg ggatcatggt tctgctgcgt
     1321 aacggtgaag agcacgccgt ggcctgggac agcatgaaat gggcgcggcc tttcttgaat
     1381 accaacagca tgggcccgcg cccacagcag ccagccgatg tcagccagat tggcgacctg
     1441 atccgcgtgc agcgccagga agacggcagc ctacgctttg ttcagttgcc cgtagcgcaa
     1501 agcgccctgg tatcgctgga cccgaataat ggcgccattc gcgccctggt cggtggcttc
     1561 tcctttgaac agagcaacta caaccgcgcc gcacaagcca agcgccagcc gggctcaagc
     1621 ttcaaaccat tcgtgtacag cgccgcgctg gataaaggct acaccgccgc caccctagtg
     1681 aatgacgcgc cgattgtgtt tgccgacgac gccctgtcgc aggtatggcg accgaagaat
     1741 gacaacaata ccttcctcgg cccgatccgc ctgcgtgaag ccttgtatcg ctcgcgtaac
     1801 ctggtttcca ttcgcctgct gcaggacctc ggcatcgaca gcaccctgag ctatatcgag
     1861 cgctttggtt tcgagaagaa ggacctgccg cccaacctgt ccctggcgct gggcaccgcg
     1921 agcctcacgc caatggaaat cgccgagggc tggagcgtgt ttgccagcgg cggttacaag
     1981 accgagcctt acctgatcga gcgcattgag gatcgcaccg gcaagcagct tttcaatgca
     2041 aacccggcgc aaactccgac cggtatcgcc cagcgcgcag aacagagcag cgccgtgcaa
     2101 ctcagcgtcg gcgaccaacc actgccgcct gagccggtac tcgccgaaca gatcatcgat
     2161 gaacgtacgg cctacatcat gaccagcatg cttcaggatg tgatcaagcg cggcactggt
     2221 cgccgtgcca tggccttggg ccgcgatgat ctggcgggca aaaccggcac caccaacgaa
     2281 tcgaaagaca gctggttctc cggctacaac gcggattaca tcaccacggt ctgggccggt
     2341 ttcgatcagc cagaaagcct gggccgcaac gaatatggcg gcaccgtggc cctgccaatc
     2401 tggatgagct atatggccgc cgcgctgaag gacaagccca gccacctgct ggcagaaccc
     2461 gaaggcctac tgaccttacg catcgatcca cacagcggcc gtgcagccac acccgatacg
     2521 ccggaagcct acttcgagct gttcaagagt gaagactcgc cgccccccat gggcgagttt
     2581 gagcctggta cgtatatacc gggcagccct ctaccggctg atgagtccgc gccaatcgat
     2641 ctgttctaaa tgactcgacg cctttagcgg cagcctgaaa agccaaaata gcgccaataa
     2701 aaaacccgca gaagcgggtt ttttattgct cttaagctaa gcgcttagcc gttgaacaca
     2761 tcatccaccg actgcagcgg gtaatgcttc ggatacggca gagtcgccac accactttca
     2821 atcgcagcct tggccactgc atcgcagacc acagtgatca ggcgagggtc cattggcttc
     2881 ggaatgatgt actcgcggcc gaacgacagg gcgatgccgc cgtaggcgtc acaaacttcc
     2941 tgcggcaccg gcagcttggc cagatcacgc agggccaggg cagcggcgat cttcatttct
     3001 tcgttgatgc gggtcgcgcg aacgtccagc gcaccacgga agataaacgg gaagcccagt
     3061 acgttgttga cctggttcgg gtagtcagaa cgaccggtag ccatgatcac gtcattacgt
     3121 acttcatgag ccagttctgg tttgatttcc gggtccgggt tggagcaggc gaacacgatt
     3181 gggctcggcg ccatgcgttt gaggtcttcc ggcgccagca ggttggcacc cgacagacca
     3241 acgaatacgt cagcaccgtc cagcgcgtca gccaaggtgc gcttggtggt ttcgctggcg
     3301 aataccgcct tgtactggtt caggtcgtcg cggccagcgt ggatcacgcc tttgcggtcg
     3361 atcataaaga tgttctcgac cttggcgccc atgctgacca gcaacttcat gcaggagatg
     3421 gcggcagcgc cggcgcccag acagacgatc ttggcgtctg gcagggtctt gccagcgatt
     3481 tccagggcgt tgagcatacc ggccgcggtc acgatagcgg tgccgtgctg gtcatcgtgg
     3541 aacactggaa tgtcgcactg ttcgatcagc gtgcgttcga tttcaaagca ctcaggggcc
     3601 ttgatgtctt ccaggttgat gccgccgaag gtgatcgaga tacggcgtac ggtgtcgata
     3661 aaggcctgcg ggctctcgga ctcgacttcg atgtcgaata catcgatacc ggcaaagcgc
     3721 ttgaacagca cgcctttacc ttccattaca ggcttggaag ccagcgggcc aagatcgccg
     3781 agaccgagaa tcgcggtacc gtcggaaatg actgccacca gattgccttt gccggtgtag
     3841 cggtacgcca gctccggatc acgcgcgatt tcgcgcacag gctcagcgac gcccgggctg
     3901 taggccaggg acaaatcgcg tgcggttgcg gtgggcttgg ttagctcgac actcagctta
     3961 cccggacggg gatgggcgtg atattcgaga gcggctgttt tcaaatcaga catgttggca
     4021 tttccgcttt gggctgttga acaggcaggc ggccgaggat acgcaagttg tatacagctg
     4081 cacaagactg gcaagtctgg aagcgcacgc aggtcctagc gcacgacttt cagccagtcg
     4141 ccagaacgcg gctgacctga agggtagagg ttattcaaca ggcgcagggt cttcagcgca
     4201 tcacccggca acttgctgcc cttggcaagc gcctcgatag tttgccccgg cttggccttt
     4261 agcatatgca ggcgcaacgg ctgcgcctct ttaaactcgg cgctggtcat cggccgaaag
     4321 cttttgatca cgctgagaaa ctgctcatcc tggctttcca gcgaggctcg accacgcacc
     4381 gcaccgacaa acaggtaaac ccgtgggcca tgctgaataa ccgccacacg cttggcggca
     4441 ttgcctggaa tcaccgcgct ataacccttg agtccagcct gctgcaactc gacgcctgcc
     4501 accagccgct ggccgccgac gcgctggcgc agcagctcag ccggtgccag cttttgcgga
     4561 ttgtcttcca gggtcatggc gataaatgcc tgctgatcag cggtgtgacc aatcagcgca
     4621 tccggacgat tgagcaggct ccaaccctgg ggaaacgcca gggtgaaatt cagatcggca
     4681 tggtagaaac gctggccgcg acgcacgcca gtttcagcgg aatcaccaaa gggcaaacct
     4741 tccagacgct tgagaaaagc ctcgcgattg acctcctgct gaccggtggc caaggcccgt
     4801 gccgggccca acacctgctg caaacgccgg tcgttatccg gatgggtatc gaacagcccg
     4861 tggtagccgc ccgcaggctg cgcctcaccg cgcgcggcgg cctggtcgcg ggcgaagtct
     4921 tcctgattct tcagcacctt gaccacttcg atcatcgcct gcgggtcata gccgctgcgg
     4981 gctaggtact gcgcacccag gccgtcggcc tccagctcca tatcgcggcc atagccacgg
     5041 acaaaagcac tgcccagcac accggtgaca tcggcagcag cacccacgcc ggtgccaatc
     5101 gccacggcct ggccgagcaa gccccaggcg gtggactgac tttgctgctg cacgctgtgc
     5161 cgcgcagtca cgtgacccac ctcgtgaccg agcacggcgg ccagttcggc ttccgagctc
     5221 agataagcca gcagtccgcg atggatatag atgtagccgc ccggcagggc aaaagcgttg
     5281 atatccggac tgtcgaccac ggtgaactga taggccagct ggttgcggtg gctgtgcttc
     5341 gccacccgct cacccacctg ctgcacatag gcctgcagct tctcgtcggc gtagcgcggg
     5401 ttctgcttga ggatttcctg gttgtatcgg cgaccgagat ccagctcctg ctgctcactc
     5461 atcatcacga agttgctgcg cccggtagcc ggattgaccg cacagccggc caactgcagc
     5521 agcagaacga acaacgtcag tacgcgcagg atcatggcaa cacctccaac atggccttat
     5581 cggtcaacgg cagcatccag cgcgcctgcc ccgcgcgcaa accgccacgc ttggagcggt
     5641 cgatcaccca gccgcgggcc tcgaccttgc gaccttcaag cgcttggaca gcacgcagat
     5701 caaaattgtc caacgcccgc ggagcaatat gcagcaccag cgaatcgccc atttccaacc
     5761 acagcccgcc gccattgcgc tcgacacgct cgacctgacc ctgcagcagg gcaaaaccgc
     5821 cgctgctcaa ctgttcggcg gcttgcaccg gggaacgctg ccagagcccg gcccgcgcct
     5881 gccgggcact gcgctcggca gcttgctggc acggcaccaa cgccagattg ggcgcgactg
     5941 caaccagata gcccaggccc tcagcgagca actgcgcctc cagattacgg ccacggttgt
     6001 cataggcgtg cgccagggtg cgcccgtagt gatccttggc ctgtttgccg tactgcagcg
     6061 aaacgcgctc atcgctggcc gccaccagcg cctgcaaacg cttgcgcgcg gcctcggcaa
     6121 aaggttccgc agagcggccc tgacgcccca tctccggagt attcaagccg atcagacgga
     6181 cattacgccc atcgaccaga cgcagggtgt cgccatccac cacacgctgc accttgacgc
     6241 taggcaagtt gcccggtgcc gggcagaacg ccagggcggc ggcggaataa acgccgaaaa
     6301 tgaaaaaggc gcccactagg gacgcctttt tcagtagcgc ggtgcaaccc atctgggtct
     6361 gcggcgctgc gcgcaagctt actgcttggc gccgaacatg ccgaaacgct gcttgaagcg
     6421 atcaacacgg ccgccggtat ccagcatttt ctgcttgccg gtgtagaacg ggtggcactc
     6481 gttgcacacg tccaggctca gcggcttggc c
//
LOCUS       DASRUM010000117         2330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2398330, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000117 DASRUM010000000
VERSION     DASRUM010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2398330"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /gene="thrH"
                     /locus_tag="VFF22_07980"
     CDS             complement(<1..518)
                     /gene="thrH"
                     /locus_tag="VFF22_07980"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HZX16785.1"
                     /translation="MEIACLDLEGVLVPEIWIAFAEKTGIESLKATTRDIPDYDVLMQ
                     QRLRILDEHGLKLADIQEVIATLKPLEGAVEFVDWLRERFQVVILSDTFYEFSQPLMR
                     QLGFPTLLCHKLITDETDRVVSYQLRQKDPKRQSVIALKSLYYRVIAAGDSYNDTTML
                     SEAHAGILFHAP"
     gene            707..1441
                     /locus_tag="VFF22_07985"
     CDS             707..1441
                     /locus_tag="VFF22_07985"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004887688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HZX16786.1"
                     /translation="MSHPFDAAELAATYANKSPQDILKLAFEHFGDELWLSFSGAEDV
                     VLVDMAWKLNKNVKVFSLDTGRLHPETYRFIDQVREHYAISIDILSPEAAALEPFVKE
                     KGLYSFYKDGHGECCGIRKTAPLRRKLSTVKAWATGQRRDQSPGTRSQVAVVEIDGAF
                     STADNTLYKFNPLAQMSSEDVWAYIRMLELPYNTLHERGFISIGCEPCTRPVLPNQHE
                     REGRWWWEEATQKECGLHAGNLIAKI"
     gene            complement(1508..1789)
                     /locus_tag="VFF22_07990"
     CDS             complement(1508..1789)
                     /locus_tag="VFF22_07990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010448985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX16787.1"
                     /translation="MSEALSIHHDLAGHQFETTVDGDRAYLAYMDLGKQTLDIYRTFV
                     PNTLRGRGIAAALTEHALQYAERLGYTVIPSCSYVERYMVRRQRQADKS"
BASE COUNT          567 a          674 c          587 g          502 t
ORIGIN      
        1 tccggcgcat ggaacaggat gccggcatgg gcctcgctga gcatggtggt gtcgttgtac
       61 gagtcaccgg cggcgatcac ccggtagtag aggctcttga gggcgatgac cgactgacgc
      121 ttggggtctt tctggcgcag ctggtagctc accacacgat cagtctcgtc ggtgatcagc
      181 ttgtggcaca gcagggtcgg gaaacccagc tgacgcatca gcggctggga aaactcgtag
      241 aaggtgtcgg agaggatcac cacctggaaa cgctcgcgca accagtcgac aaactccacc
      301 gcgccctcca gcggcttcag ggtggcgatc acttcctgaa tgtccgccag tttcaagccg
      361 tgctcatcga gaatgcgcag acgttgctgc atcagcacgt cgtagtccgg aatatcccgg
      421 gtggtcgctt tcagcgattc gatgccagtt ttttccgcga aagcgatcca gatttccggg
      481 accaacacgc cttccaggtc gagacacgcg atttccacag gacactccta gcttggattt
      541 atttgagagg cggcactcta gcggctaccc caacgctctg caacgcagag ctttataccc
      601 aaatcagagg tctactacgg cacttggtta tttagtcgta taaccggctt ttgctaccat
      661 cgctacacaa gacgcgcgac gtcaccataa ctaggaattc aagccgatga gccacccctt
      721 cgatgcagcc gaactggcgg cgacctacgc caacaagtcg ccacaagaca ttctcaaact
      781 cgccttcgag cattttggcg atgagctgtg gctgtccttc agcggcgcgg aagatgtggt
      841 gctggtcgac atggcctgga agctcaataa gaacgtcaag gtcttcagcc tcgacaccgg
      901 acgtctgcat ccggaaacct accgctttat cgatcaagtg cgtgagcact acgccatcag
      961 catcgacata ctctcgccgg aagccgcagc actcgagcct ttcgtcaaag aaaaaggcct
     1021 ttatagcttc tataaagacg gtcacggcga atgctgtggt atccgcaaga ccgccccgct
     1081 acgccgcaaa ctttctacgg taaaagcctg ggccaccggc caacgccgcg accagagccc
     1141 aggcacacgc agccaggttg ccgtggtgga aatcgacggt gccttttcca ctgccgacaa
     1201 caccctgtac aagttcaatc cgctggcgca gatgagcagc gaagatgttt gggcctatat
     1261 ccgcatgctt gagctgccct acaacacctt gcatgaacgc ggctttatca gcatcggttg
     1321 cgagccctgc acccgtccag tgctgcctaa tcagcatgag cgcgaggggc gttggtggtg
     1381 ggaagaagcc acacagaaag aatgcggcct gcacgcaggc aatctaatcg ccaaaatctg
     1441 acaacgaagg cccgtgatag atacaagaag gccagtcatc gactggcctt tttcttgtct
     1501 gcagtattta gcttttatct gcctggcgct gacggcgcac catataacgc tccacataag
     1561 agcacgaagg aatcaccgtg tagcccagcc gctcggcata ctgcagtgca tgctcagtca
     1621 atgcagctgc gattccgcgc ccacgcaacg tgttagggac gaaggtgcgg taaatatcca
     1681 gggtttgctt tcccagatcc atgtaagcca gatacgcccg atcaccatcc actgtggtct
     1741 cgaattgatg gcctgccaga tcatggtgaa tggacaacgc ctcgctcatc tcagctcctc
     1801 tgttaactcg cgtaacccgt tcgcacttgt gcagcagccg ggctacagca cctggcacct
     1861 attaagttgt aggcaattat cacgagctta tcaggccaaa acctgcgagg tcacgtgaat
     1921 caataactta gatcgcctag cgcggaattt cgacactcac acaagccatg cattggctct
     1981 atcagcaacg atgtagcccc tgctccattc ggcaattcat gctcgaaaac taccggtatc
     2041 atttttcagc tactgtccat agccagcgca gctacaacgt gacaataacc actgcgcaaa
     2101 gaactgccaa acgcgctaga actgtttgtt ttttaaccaa aagatgccca ataagtggcc
     2161 ggttatgcat taaacggaaa aaaactctag cggcttgcct tgcctccgtt tacttactac
     2221 aagttacagg tagtatgtac gccggctaat ttcctaccat taggaaattg cttttatgga
     2281 aaaactccac ctcaagggga acacgatgaa caacgttctg aaattctctg
//
LOCUS       DASRUM010000118        11749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_731909, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000118 DASRUM010000000
VERSION     DASRUM010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 11749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11749
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731909"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..522)
                     /locus_tag="VFF22_07995"
     CDS             complement(85..522)
                     /locus_tag="VFF22_07995"
                     /inference="COORDINATES: protein motif:HMM:NF025473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABA4-like family protein"
                     /protein_id="HZX16788.1"
                     /translation="MAPLKPLFKLTSLIALGGWLMLVCLPFWSYTDELVLGLSVVLLA
                     IVYSWLLFVAMRQRPEPGSGRPGFFSLGGVLSLLRQPTAALAAWVHILAFDLMVGLYI
                     RHEGAIAGISHWALLPCYVLTLMFGPLGLLAFLGLRWGLVMAA"
     gene            complement(647..1306)
                     /gene="msrA"
                     /locus_tag="VFF22_08000"
     CDS             complement(647..1306)
                     /gene="msrA"
                     /locus_tag="VFF22_08000"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HZX16789.1"
                     /translation="MTAITSIWRSRLLASSILALGGLLAACEPTAAQAPQPQASAPTV
                     ENPGVAIFAGGCFWCTESDFDKVPGVIETTSGYIGGHVDNPTYEQVSAGSSGHIEAVQ
                     VRFDPSKTSYAKLLEAFWPTIDPITANAQFCDRGPQYRSAIFYSNAEEQQLAEASKAA
                     LEKSGRLPAPVVTEILAASTFYPAEDYHQDYYQRNPLRYNYYRNGCGRDQRLEELWGK
                     K"
     gene            complement(1309..1824)
                     /gene="msrB"
                     /locus_tag="VFF22_08005"
     CDS             complement(1309..1824)
                     /gene="msrB"
                     /locus_tag="VFF22_08005"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZX16790.1"
                     /translation="MKRRSLLKGMALLPALPLLGHAPFSLAQAIASSVTPLNKPHSDW
                     RDLLTPEAYAVLFEEDTEAPGSSPLNQEKRDGTYLCAACYLPLFASQDKYESGTGWPS
                     FTQPIAGHMGTKRDFKLFFPRTEYHCARCGGHQGHVFDDGPKPRGERWCNNGVALRFI
                     PQGEALPELRS"
     gene            complement(1943..2980)
                     /locus_tag="VFF22_08010"
     CDS             complement(1943..2980)
                     /locus_tag="VFF22_08010"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZX16791.1"
                     /translation="MRISRLLRRLRNNFQLSIITLMGLFGVIGITPYAVYRLATGNYL
                     VGSADTVIVVSTVLAVLYSWRTGDTIKPGIYLATIFSAGATLIAINLGVNGLFWIYPL
                     ILFNFFMVSPAKALLATILVLTTLVGYSQLNPGSVFESDYQMVSFLVTSMLASMLSFV
                     FAYRTSSQRDQLQQLAIHDPLTGARNRRAMNEELKIAASIYRRHGNSYGVLVMDLDHF
                     KRINDNHGHQAGDQVLVDFVDLIKRSSRKEDRLFRFGGEEFLLLLPNTERAGLLAAAQ
                     HLQQQVAQHMRGPSGAVTMSVGGAILRRGEHWESMLQRADERLYRAKNAGRDCIVIDD
                     GVDKTSSLPIA"
     gene            complement(3111..3545)
                     /locus_tag="VFF22_08015"
     CDS             complement(3111..3545)
                     /locus_tag="VFF22_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX16792.1"
                     /translation="MHYRLLPAPLKPLADKFYRSQRSAMRATAGAQLWVAEDREIVAA
                     LCLHQVAHGHWLTSLLVDSTRRGQGVARQLIETALADCATVVWLFCHPQLGDFYLRLG
                     FVLCNDLPQPLAERLSRYQRSKSLLAFARMPCPSSAAQGLEP"
     gene            4110..5423
                     /locus_tag="VFF22_08020"
     CDS             4110..5423
                     /locus_tag="VFF22_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HZX16793.1"
                     /translation="MLLLLSACCLACQPLSALAWSNHSLGSQLALQAVPELQRTLSYE
                     PLEAFLDAQAPAIEQLLDQQEAFARVHFVDYPARPDDLRFSATTITAADRRSAFLQAL
                     RVNPQIRLAGFIQQLPGQADDGRPRLAAEQVLVFRKIGPWSHWRYLALQPGEAVSAVQ
                     VLSSAADEPDYGHDINLFSDNPGGVGAHYNFGAQPFGDARFEYSSQAPFHIGYYHEPE
                     LVLQAAPYLRRTLPHWRIYQYTSLARLALESGHDYWGYRFLGWALHYMQDLTQPYHAK
                     TVPGFATAELMLIALKAASGYPEDRLATIARVADRHTAIEEYQLERMQQLMDKQATSP
                     LLQAYTQSPQDIYPAYSVDYAQQVVTAESYASADAFDELIGQWLARKPSSAPGFSGGN
                     QLDQAEADPLLEQLLVELFKRFGAHTRNVVQTTLAQPAPSVPAAQ"
     gene            complement(5401..5580)
                     /locus_tag="VFF22_08025"
     CDS             complement(5401..5580)
                     /locus_tag="VFF22_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16794.1"
                     /translation="MERERALVNLEHNDRLDVAWSMTSHECEQRQLIELHNPLLPSDT
                     PLQRRELWFTAPPVR"
     gene            complement(5609..7351)
                     /locus_tag="VFF22_08030"
     CDS             complement(5609..7351)
                     /locus_tag="VFF22_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZX16795.1"
                     /translation="MFDVLIKNALFFDGSGTPGVLRHLGIRDGKLACISASALDESGC
                     GQVIDAQSRWVTPGFIDMHTHYDAELVAAPALKESVRHGVTTVMIGSCSISMVLSSPE
                     DCSDLFTRVESVPREHVLPLLQARKTWNSAGEFAVFLDQHPLGPNICSFLGHSDLRVA
                     VLGLERAVDAKVRPSENELQQMERLLEDALDAGLLGLSSMTNPWDKLDGDRQRSKSLP
                     SVYAPWAEYARLNKILRRRGRIHQGAPNLVTKFNVLAFLWDSIGGWWRKPLKTTLITL
                     MDVKADPWLAPILGPLTRWINRLSNADFRWQTLPVPFETYADGMEFVVFEEFPAGQAA
                     LHLASHDMRSQLFQDPAYRLRFRQDVEKKFGPRVWHRDFDDAWIVDCPDASVVGKSVG
                     ELAKARDEHPADLFLDLLVAHGPRLRWRTLIANHRPEVVEKLVCEPSTLIGFADSGAH
                     IRNMAFYNFGVRLLKLVRDAELRGQAVMPIETAIWRLTGELGEWFDIEAGRLYEGARA
                     DLVIIDPTALDESLASYQEAPMEAFGGLQRMVNRGAAVDAVLINGRIAFQQGEFASDL
                     GHARGYGQFLRSNA"
     gene            complement(7468..7634)
                     /locus_tag="VFF22_08035"
                     /pseudo
     CDS             complement(7468..7634)
                     /locus_tag="VFF22_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611586.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(7718..7948)
                     /locus_tag="VFF22_08040"
                     /pseudo
     CDS             complement(7718..7948)
                     /locus_tag="VFF22_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611586.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(8100..8426)
                     /locus_tag="VFF22_08045"
     CDS             complement(8100..8426)
                     /locus_tag="VFF22_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020288845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2025 family protein"
                     /protein_id="HZX16796.1"
                     /translation="MSITSASICAAADQLQGFVGFNAKTGRYIVRFSEDSFGLDVLEQ
                     SITPTCEFVWRAFEGELMNLDRARLQLLLEQNIDDRLNISEPLRVYVRRTDLPEISAE
                     RRRLNA"
     gene            complement(8605..9225)
                     /locus_tag="VFF22_08050"
     CDS             complement(8605..9225)
                     /locus_tag="VFF22_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16797.1"
                     /translation="MHKFLILAACLLLGACASNPQTTYQQEVKAGKSPMAKSALEAIH
                     NTQAIPLPLNTGLLAPQWPISADEPRLDFGSSISNYRIFSLQLKKGERYTLNVHSLCN
                     KPCMGVSKYALKPRAMLLDAYGAVIADKPSHAEAVVGQISLSWDGEATEDGTYYLLVA
                     ADNDDIGQTIVIDDVWVNNSPLMAVKVGMQSSPFGKISAFSSTRKD"
     gene            complement(9415..10116)
                     /locus_tag="VFF22_08055"
     CDS             complement(9415..10116)
                     /locus_tag="VFF22_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZX16798.1"
                     /translation="MGAQWKAKPKEAAANARGKIFGRLVKEIMIAARNGADPDLNPKL
                     RLAIHQAKKASMPKETLDRAIKKGAGLLGDTVTFHSATYEGFAPHQVPVIIECLTDNI
                     NRTVAEIRVLFRKGQMGASGSVTWDFDHVGMIEASTESGADPEMAAIEAGAQDFEPAD
                     EGNTLFITEPTDLDAVCRALPEQGFNVNSAKIGYIPKNPVSSLTAEQLEEVEAFLEAL
                     DAHDDVQNVYVGLSG"
     gene            10317..11201
                     /locus_tag="VFF22_08060"
     CDS             10317..11201
                     /locus_tag="VFF22_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZX16799.1"
                     /translation="MQPKDLLLALVVIIVWGMNFVVIKIGLDNMPPMLLGALRFLLAA
                     FPAVLFIKRPQIPLRWLLAYGLTISLGQFAFLFSAMSVGMPAGLASLVLQSQAFFTLL
                     FAVMFLGERFRVVNLFGLLVAAGGLLLIGAQGNGLMTLAGFLLTLCAAAMWALGNIVT
                     RKLGKVNLVGLVVWGSLIPPLPFFALSWLLEGPDAIEAALRGFSLNSMLVLIYLAFGA
                     TILGYGLWSRLLSRYPASQVAPFSLLVPVVGITSAALLLDERLSTLQLLGALLVMLGL
                     LLNVCGGWLQERLRARWA"
     gene            complement(11206..11457)
                     /locus_tag="VFF22_08065"
     CDS             complement(11206..11457)
                     /locus_tag="VFF22_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004878611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16800.1"
                     /translation="MTQTPAAEPTLTVQPADSAAPSASENTSTSAAKTSGFASLFGAA
                     KAASGKQDDQPWHQKGNKSSHEKKIGPAPSGTRRAMGKR"
     gene            complement(11610..>11749)
                     /locus_tag="VFF22_08070"
     CDS             complement(11610..>11749)
                     /locus_tag="VFF22_08070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZX16801.1"
                     /translation="LGSFGPGQMVKEFDTVVFSAPINVVQGPVKTQFGYHLLEVTSRQ
                     D"
BASE COUNT         2100 a         3273 c         3839 g         2537 t
ORIGIN      
        1 agcaaacgat tgagtacccc aatggctacc gggtgcagca gtatccaggg cagagcactg
       61 aatactatga gcgcagccgc tagctcaggc cgccatcacc aagccccagc gcaagcctag
      121 aaacgccagc aggcccagtg gaccgaacat cagggtcagt acatagcagg gcagcaatgc
      181 ccagtggctg atgccggcaa tggcgccttc atggcggata tacagcccga ccatcagatc
      241 gaacgccagg atatgcaccc aggctgccag cgcggcagtg ggttggcgca gcagtgagag
      301 caccccgccg aggctgaaga agccgggtct gccgctaccc ggttcgggcc gttggcgcat
      361 ggcgacaaac agcagccagc tatagacgat ggccagcagg accacgctga ggcccagtac
      421 caactcatcg gtataggacc agaacggcag acagaccagc atcagccagc cgcccagggc
      481 gatcagcgag gtgagtttga acaggggttt caacggggcc atggcgcgct cactgagcaa
      541 ggtgtgagct caaccatagc ggatacggtg attgccgcat cctgatatca gcggctgaat
      601 acagcgccat cagctggcgt gagggccagc tgataggcgc gttgtattac ttcttgcccc
      661 acagctcttc caggcgttga tcacggccgc aaccgttgcg gtagtagttg tagcgcagcg
      721 gattacgttg gtagtagtcc tggtggtagt cctcggccgg gtagaaggtg ctggcggcga
      781 ggatttccgt gaccaccggc gccggcaggc gcccggattt ctccagggcc gctttcgatg
      841 cttcggccag ttgctgttct tcggcgttgc tgtagaagat cgcgctgcgg tactgcgggc
      901 cgcggtcgca gaactgggcg ttggcggtga tcgggtcgat ggtgggccag aaggcttcca
      961 gcagcttggc gtagctggtc ttgctcggat cgaagcgcac ctgcaccgct tcgatatgcc
     1021 cgctgctgcc ggccgagacc tgctcgtagg tggggttgtc gacatgcccg ccgatataac
     1081 cggaggtggt ttcaatcact ccgggcactt tgtcaaagtc cgactctgtg caccagaagc
     1141 agccgccggc aaagattgcc acgccggggt tttccaccgt cggcgcactg gcttgtggct
     1201 gcggcgcctg cgcggcggtg ggttcacaag cagccaatag gccgcccaga gcaagaatgc
     1261 tgctggcgag cagacggctg cgccagatag aggtgatggc agtcatgttc agctccgcag
     1321 ctcaggtaag gcttcacctt gggggataaa gcgcaaggcc acaccgttat tgcaccagcg
     1381 ttcgccgcgc ggtttcgggc cgtcatcgaa gacatggcct tgatgcccgc cgcagcgtgc
     1441 gcagtggtat tcggtgcgcg ggaagaacag cttgaagtcg cgcttggtgc ccatatggcc
     1501 tgcaatcggc tgggtaaagc tgggccaacc ggtgccgctt tcatatttgt cctggctggc
     1561 gaacagcggc aggtagcagg cggcgcagag gtaggtgccg tcgcgttttt cctgattaag
     1621 cgggctgctg ccgggggctt cggtgtcttc ttcgaagagc acggcatagg cttcgggcgt
     1681 cagcaggtcg cgccagtcgc tgtggggttt attcagcggc gtcacgctgc tggcgatggc
     1741 ttgcgccagg ctgaacgggg cgtgaccgag caggggcagg gctggcaaca gggccatccc
     1801 cttgagcagg gagcgtcttt tcatggcagt gctctgtgtg aggtgtggcc aatagacaga
     1861 gacaaagccg agttgttaca gctttgtcgg cagcgcgcga gaatttttcc gcgcaagttg
     1921 taagcaaaga taaaccatgc ggttaggcaa taggcaggct gctggttttg tcgacgccgt
     1981 catcgatgac gatgcagtca cgcccggcat tcttggcgcg atacaggcgt tcatcggcgc
     2041 gctgcagcat gctttcccaa tgttcgccgc ggcgcaggat ggccccgcca acggacatgg
     2101 tcaccgctcc gctaggcccg cgcatgtgct gcgccacttg ctgctgcagg tgttgcgcgg
     2161 ccgccagcag gcccgcgcgc tcggtgttgg gcagcagcag gaggaactct tcgccgccga
     2221 agcggaacag ccggtcttcc ttgcgggatg aacgtttgat cagatcgaca aaatccacca
     2281 gcacctgatc gccggcctgg tggccatggt tgtcgttgat ccgtttgaaa tgatccaggt
     2341 ccatcaccag cacgccgtaa ctgttgccgt ggcggcggta gatggaggcg gcgatcttca
     2401 gctcttcatt cattgcccgg cggttgcggg caccggtcaa ggggtcatgg atggccagtt
     2461 gttgcagctg gtcgcgctgg ctgctggtgc ggtaggcaaa gacgaagctg agcatgctgg
     2521 ccagcatgct ggtgaccaga aatgaaacca tctggtagtc gctttcgaac accgagccgg
     2581 ggtttagctg gctgtagccg accagggtgg tgagcaccag aatggtggcc agcaaggcct
     2641 tggcgggcga caccataaag aagttgaaga gaatcagcgg gtagatccag aacaggccgt
     2701 tgacgcctag attgatggca atcagcgttg cgccggcaga gaagatggtg gccaggtaaa
     2761 taccgggttt gatcgtgtca ccggtgcgcc aggagtaaag caccgcaagc acggtggaaa
     2821 ccacaatcac ggtgtcggcg gagcctacca ggtagttacc ggtggctagc cggtagacgg
     2881 catagggggt aatgccaatc acgccaaaca ggcccatcaa ggtaatgatg gacagctgga
     2941 agttgttacg caggcgcctg agcaaccgcg aaatccgcat aggtaaaggc cattgttgtc
     3001 gttgttatga gctaagcatg ccgagcttgt cctggccata gcaatagtaa aatccagcaa
     3061 agtgtcggca tcagccgcaa tctgccgaat ggcgtttggt gggcctggtg ctaaggctcc
     3121 agcccttgtg cggcagagct tgggcaaggc atacgagcga atgccagcag gcttttactg
     3181 cgttgatagc ggctcagacg ctcagccaga ggctgcggca ggtcgttgca gaggacaaag
     3241 cccaggcgca ggtagaagtc gccgagctgc ggatggcaga acagccagac cactgtggcg
     3301 caatcagcca gcgcggtttc gatcaactgg cgcgccacac cttgcccacg ccgcgtgcta
     3361 tcgaccagca ggctggttaa ccaatgcccg tgcgcgacct ggtgcaggca gagtgcggcg
     3421 acgatttccc gatcttctgc cacccatagc tgtgcgccgg ccgtggcgcg catggctgag
     3481 cgttggctgc ggtagaactt gtcggcaagt ggtttgagcg gggcggggag taggcggtag
     3541 tgcattacag gtactgttgc gacggttagt ggatgcctga gcgcaagcgc gatgcttgcc
     3601 tcagaccggt ggctcagacg ttggcggcgt ggctgccgag cagggttttc gcaccttgct
     3661 tgtctttctt gtctatgcgc ttttcgctgg cggttttcag cggctttttc ttcacttgtt
     3721 tctttgaatc catacctttg ctcatgatct tcacctctga caatgtcgtg gttgaacatt
     3781 ctggagcggg ccaggccctc gcggtgaggc gaggttggta cttggagtct acgcccatgc
     3841 tgggcgaata gctggggcgt tatccgggcg tttggatgca agcctgggcc gcgatgatca
     3901 gccgctgtag gtgggtttgc tgcaaggcat tctgggtgag catgaattgt tgctgcggtg
     3961 tttgcgcttt agctcaatct gtctgggcat gcagcgtgct gctcgattag tgaatggact
     4021 gacttgatac ggcagtgcca gatggctgtt ttggcgttag gctacgcgtc tttgtcctgc
     4081 catccgctgt cggagtctcc gtgaagccat tgctcctgtt gctatcggcc tgttgcctgg
     4141 cgtgtcagcc gttaagcgcg ctggcctggt ccaatcactc gctgggtagt cagctggcgt
     4201 tgcaggccgt gccggagctg caacgcacct tgagctacga gccgttggag gcgtttctcg
     4261 atgcccaggc tccggccatc gagcagttgc tggatcagca ggaagccttt gcccgcgtgc
     4321 attttgtgga ttacccggcg cgtccggatg acttgcgctt tagtgcgacg acaataactg
     4381 ccgctgacag acgcagtgcg tttctacagg cgttacgggt caatccacag attcgtctgg
     4441 ccggtttcat tcagcagttg ccggggcagg ccgatgatgg tcggccgcgt ttggctgccg
     4501 agcaggtatt ggtgtttcgc aagatcggcc cttggagcca ttggcgttac ctggccttgc
     4561 agccgggtga ggcggtgagt gccgtgcagg tgctgagcag tgcggccgat gagccggact
     4621 atggccatga catcaatctg ttcagcgaca accctggcgg agttggcgcg cactacaact
     4681 ttggcgcgca gccctttggc gatgcgcgct tcgagtattc ctcccaggcg ccattccata
     4741 ttggctacta ccacgaaccg gagctggtgt tgcaggcggc gccttatctg cgccgcactt
     4801 tgccgcactg gcgcatctac cagtacacca gtctggcgcg actggccctg gaaagtggcc
     4861 acgattactg gggttaccgt tttctcggct gggcgctgca ttacatgcag gatctgactc
     4921 agccttatca cgccaagacc gtgccgggct ttgccacggc cgagttgatg ctgatcgccc
     4981 tcaaggcggc cagcggttat cccgaagatc gcctggcgac cattgcccga gtggcggacc
     5041 ggcacacggc gattgaggaa tatcagctgg agcgtatgca gcagctgatg gataagcagg
     5101 cgacctcgcc tctgttgcag gcctatacgc agagcccgca ggacatctat ccagcctatt
     5161 cagtggacta cgcgcagcag gtggtgacgg ccgagtccta tgccagtgcc gatgcctttg
     5221 atgagttgat tggccaatgg ctggcgcgca agccgtcatc ggctccgggt tttagcggtg
     5281 gtaaccagct ggatcaggct gaagctgatc cactgctgga gcagttactg gtggagctgt
     5341 tcaagcgctt tggtgcgcac actcgcaacg ttgtgcaaac caccctggcg cagcctgcgc
     5401 ctagcgtacc ggcggcgcag tgaaccacag ctcgcgccgc tgcaagggcg tgtcgctggg
     5461 taacagcgga ttatgcagtt cgatcagttg gcgctgttcg cactcatggc tggtcatgct
     5521 ccaggccaca tcgaggcggt cgttgtgctc cagattaacc agcgcgcgct cccgctccat
     5581 gctttaggtc gcatgccctg aagccagact acgcgttgct gcgcagaaac tgcccgtagc
     5641 cgcgtgcatg ccccagatcg ctggcgaact cgccctgctg aaaggcgatg cgcccgttga
     5701 tcagcacggc atccaccgcc gcgccacggt tgaccatgcg ctgtaggccg ccaaaggcct
     5761 ccatcggtgc ttcctgatag ctggccaggg attcatccag cgccgtcgga tcgatgatca
     5821 ccagatcggc acgagcgccc tcataaagcc ggccggcctc gatatcgaac cattcgccca
     5881 actcgccggt caggcgccag atcgcggttt cgatcggcat caccgcttgg ccgcgcagct
     5941 cggcgtcgcg taccagtttg agcaggcgca cgccgaagtt gtagaaggcc atattgcgga
     6001 tatgcgcgcc ggagtcggca aagccgatca gcgtgctggg ctcgcacacc agtttctcta
     6061 ccacttcggg gcgatggttg gcgatcaggg tgcgccagcg caagcgcggg ccgtgggcca
     6121 ccagcagatc gaggaataga tcggcggggt gttcgtcgcg ggccttggcc agttcgccaa
     6181 cgcttttgcc gaccacgctg gcgtccgggc aatcgacaat ccaggcatcg tcgaagtcgc
     6241 ggtgccagac ccgtgggccg aatttcttct ccacgtcctg gcgaaagcgc aggcggtagg
     6301 ctgggtcctg gaacagctgg ctgcgcatgt catggctggc caggtgcagg gcggcctggc
     6361 cagcggggaa ttcctcgaaa accacgaact ccataccgtc ggcataggtt tcgaacggta
     6421 ctggcagggt ttgccagcgg aagtcggcgt tactcaggcg attgatccag cgcgtcagtg
     6481 ggccgaggat cggcgctagc caagggtcgg ctttgacgtc catcagggta atcagggtgg
     6541 tcttcagcgg tttgcgccac cagccgccga tgctgtccca gagaaaggcc agcacgttga
     6601 acttggtcac caggtttggc gcgccctgat ggatgcgtcc gcgccggcgc aggattttgt
     6661 tcagccgcgc gtactcggcc cagggtgcat acaccgaggg cagagatttc gagcgctggc
     6721 ggtcgccatc cagtttgtcc caggggttgg tcatgctcga cagaccgagc aggccggcgt
     6781 ccagtgcatc ttccagcaag cgttccatct gctgcagttc gttttcgctg gggcgtacct
     6841 tggcgtctac cgcgcgctcc aggccgagca cggcgacgcg caggtcggag tggccgagga
     6901 aggagcagat attcggcccc agcgggtgct gatcgagaaa gacggcgaat tcgccggcac
     6961 tgttccaggt cttgcgcgcc tgcagcagcg gcagcacatg ttcgcgtggt actgactcga
     7021 cgcgagtgaa caggtccgag cagtcttccg ggctggacag caccatgctg atcgagcagc
     7081 taccgatcat caccgtggtc acgccatggc gcaccgactc cttgagcgca ggggcggcca
     7141 ccagttcggc gtcgtaatgg gtgtgcatgt cgataaagcc gggcgtcacc cagcggcttt
     7201 gcgcatcgat cacctgacca catccgcttt catccagggc gctggcgctg atgcaggcga
     7261 gcttgccgtc gcggataccc aggtggcgca gcactcccgg tgtgccactg ccatcaaaaa
     7321 acagcgcgtt cttgatcagt acatcgaaca ttgcaggccc ctaagcgaat gaaatcggcg
     7381 tgtcgagcat agtgctaatc ccccaatttt tagtactcgc cagaagtaga ggtcaggccg
     7441 cgagggccaa tttctgtttg gatggatgcc taccaggacc atatttggtc gttcatgatt
     7501 gttatgtcca gatccatcgc atggcgtgtt cttgtacctc agcgatggac ttgaaaaggt
     7561 aatgccccag ccagtacgta acgtaccgtg cggttgtaac gttcgacgca ggcattctgc
     7621 tggggattgc tggggcttga tatgtgctag gcggatgcca tgcatctctg cccacagcac
     7681 acgcaccact cgttctgccg gcagtgatag gtcgatggcc tttcacattg cgcaggtaca
     7741 ggaagcacag gccaaaaccc cagttgcgct gattgtgcgt aagtcggatc agatggtcgg
     7801 caatttcggc attttccgaa gacagccgcg gccggtagcg atagcagttg acgctaacct
     7861 cgaaggcctg gcatgccagt ttgatgctgg ctcgaccaga gcgaacggca cgctacgcca
     7921 tctctcgcag ccgagatggc ttcaccacca cgcaaaattt ccttgatccg attaggagaa
     7981 aattctactt ctgacgagcg ctgatttcag gggattacct ggtcttctgt agacagtcgc
     8041 tgtggggggg ggaagtgaac aacccaaggt ctattcgacc atcactgctt gttcagtgct
     8101 tatgcgttaa gacgacggcg ctcggcgctg atttccggta ggtccgtgcg gcgcacgtag
     8161 acccgcagcg gttcgctgat gttcaggcga tcatcgatat tttgctccag cagcaattgc
     8221 aggcgcgcgc ggtcgaggtt catcagctcg ccttcaaagg cgcgccagac gaattcgcag
     8281 gtgggcgtga tgctctgttc gagcacgtcg agaccgaagg aatcttcgga aaaacgcacg
     8341 atataacgcc cggtcttggc attgaaacca acaaaaccct gcagttggtc ggcagccgcg
     8401 cagatgctgg cagaggtgat gctcatggaa acctcgtcga tggcagtgga agtcatcggc
     8461 ataggctaag gcgccagggg cagagcatct tgcgatggat caataaatgg gggcgcggag
     8521 caccacttgc gggcaggttg aaacgggtag ttttgatgtg agagggcgct gcctggcgag
     8581 ccattgcagc tcgccatggt gtgttcagtc tttgcgcgtg ctggaaaatg cgctgatctt
     8641 gccgaacggc gagctttgca taccaacctt gaccgccatc agtggcgagt tgttgaccca
     8701 gacatcgtcg atcacgatgg tctggccgat gtcgtcgtta tcggcggcca ccagcaggta
     8761 gtaggtgccg tcttcggtgg cttcaccatc ccagctcagg ctgatctggc cgaccacggc
     8821 ttcggcatgc gagggcttgt cggcaatcac cgcgccgtag gcatccagca gcatggcgcg
     8881 tggcttcagg gcatatttgc tgacgcccat gcacggcttg ttgcacagcg agtgcacatt
     8941 gagggtgtag cgctccccct ttttcagctg cagggaaaag atccggtagt tggagatcga
     9001 gctgccgaaa tccaggcgcg gctcgtcggc gcttatcggc cactgtgggg ccagcaggcc
     9061 ggtattgagt ggcagcggga tggcctgggt gttgtggatg gcttccaggg cgctcttggc
     9121 cattgggctt ttgcccgcct tgacctcttg ctggtaggtc gtctgcgggt tgctggcgca
     9181 ggcgccgagc agcaggcagg cggccagaat cagaaacttg tgcacggtaa aactcctgtg
     9241 tgacaacgtc cttgtgtaca cgccgggcgc tggtggcgcg gcgggcggcg attctactcg
     9301 ctttgcgcga tgcgcggagt ggtcaatctg ctagtacgac gaactgcgca cattaatctg
     9361 tgccgctcgt gggcccgcgg cgcatcctgc gcggcgggcc tggtcagcct gtatttagcc
     9421 ggaaagcccg acatagacgt tctgcacgtc atcgtgagcg tcgagcgctt cgaggaaggc
     9481 ttcgacttct tccagttgct cggcagtcag gctgctcacc gggtttttcg ggatatagcc
     9541 gatcttcgcc gagttgacgt tgaaaccctg ttccggcaac gcgcggcata cggcgtcgag
     9601 gtcggtcggt tcagtgataa acagggtgtt gccttcatcg gccggctcga aatcctgagc
     9661 gccggcttcg atggcggcca tttccgggtc agcaccgctc tcagtgctgg cctcgatcat
     9721 gccgacgtgg tcgaaatccc aggttaccga accggaagcg cccatctggc ccttgcggaa
     9781 cagcacgcgg atttccgcta cggtgcggtt gatgttgtcg gtcaggcact cgatgatcac
     9841 gggcacctga tgcggggcaa aaccctcata ggtggcggag tggaaggtca cggtgtcgcc
     9901 cagcaggccg gcacctttct tgatcgcccg gtcgagggtt tccttgggca tcgaggcttt
     9961 tttcgcctga tggatggcca ggcgcagctt ggggttcagg tccggatcgg cgccgttgcg
    10021 agcagcgatc atgatttcct tgaccaagcg gccgaagatc ttgcctctgg cgttggccgc
    10081 ggcttcttta ggtttggctt tccactgggc gcccatgggg tatctcgtta ttactggttg
    10141 ataggtgagg tggcgattct agcccttcga ggcgtttgcg gcacctatgg cgctgcaatc
    10201 tgccaagtgg tcataccgca gagcctgctg tacggctgat ttgccgggcc gctgtatggg
    10261 cgttgcgacc gtgctggcag ctctaataag gcttggtgtg ttgttgagga attgtcatgc
    10321 aacccaagga tttgttactg gccctggtgg tgatcatcgt ttggggcatg aattttgtgg
    10381 tgatcaagat cggtctggat aacatgccgc cgatgctgct gggcgcgctg cgctttctgt
    10441 tggcggcctt tccggcggtg ctgtttataa agcgcccgca gattccgctg cgctggctgc
    10501 tggcgtatgg actgaccatc tcgctggggc agtttgcttt tctgttctcg gccatgtcgg
    10561 tgggcatgcc agcggggttg gcgtccttgg tactgcagtc gcaggccttc tttactctgc
    10621 tgtttgccgt catgtttctg ggcgagcgct ttcgcgtggt caacctgttc ggcttgctgg
    10681 tggcggcagg agggttgctg ctgattggtg cgcagggcaa tggactgatg acgttggccg
    10741 gctttctcct gacgttgtgc gcagcggcca tgtgggcgct gggcaatatc gtcacgcgca
    10801 agctgggcaa agtgaacctg gtcggtctgg ttgtttgggg cagcctgatt ccgcctctac
    10861 cgttctttgc gctgtcctgg ctgttggagg ggccggatgc gattgaggcg gcgctgcgtg
    10921 gtttcagcct gaacagcatg ctggtgctga tctatctggc gtttggcgcg accatcctcg
    10981 gttacggtct gtggagtcgc ttactctcgc gctacccggc cagccaggtg gcaccgtttt
    11041 cgctgctggt gccagtggtg gggatcacgt cagcggcatt gctgctcgat gagcgcctga
    11101 gtaccttgca gttgttgggc gcactgctgg tgatgctcgg cttgctgctg aatgtctgcg
    11161 gcggctggct gcaggagcgt ttgcgcgcgc gttgggccta gatttttagc gtttgcccat
    11221 ggcgcggcgg gtaccgctcg gcgctggacc gatcttcttt tcgtggctcg acttgttgcc
    11281 cttctggtgc cagggctggt cgtcctgttt gccggaagcg gctttggcgg cgccgaatag
    11341 cgaggcaaaa ccactggttt tcgctgcgct ggtgctggtg ttttcgctag cggacggtgc
    11401 ggcgctgtct gcaggctgaa cggtgagggt gggttcagct gctggagtct gggtcatggc
    11461 aaggtccgcg ggtgcgagaa taaaaggccg gcgagtatac ctggctattg cccgaggttt
    11521 gcggttgttg gtcagtggcg ctgtgcctgg cggcgctgcg ctaaacgaag ggattcgccg
    11581 ggcctaggcg gcccagcgaa caggcaggat cagtcctggc ggctggtcac ttccagcagg
    11641 tgataaccga actgggtctt caccgggcct tgcaccacgt tgatcggtgc gctgaatact
    11701 acggtgtcga attccttgac catctggcct gggccgaacg agccaaggt
//
LOCUS       DASRUM010000119         5283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_575252, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000119 DASRUM010000000
VERSION     DASRUM010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575252"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..887)
                     /locus_tag="VFF22_08075"
     CDS             complement(183..887)
                     /locus_tag="VFF22_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZX16802.1"
                     /translation="MSKFTWFSGRGPALTQLQLERRGKLAEPGAFDERPLHQQRFVIL
                     DLETSGLNMRRDQVLSIGAVVIENGAIDLSQQFECTLQRDGHQASASTLIHGIAPNEI
                     ANGEDPAEALLSFMEFVGDSPLLAFHAEFDQRMLARALKQSLGYRLRHAFFDVAEIAP
                     LVCPKANLPHAGLDDWTEHFGLQVQQRHHASADALVTAELALILFNKARQQQLDNLPA
                     LLQQLNSRRRQIAHSI"
     gene            complement(884..2815)
                     /locus_tag="VFF22_08080"
     CDS             complement(884..2815)
                     /locus_tag="VFF22_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HZX16803.1"
                     /translation="MSSADAIAQAGKTAVLQNIHGTMAFLQKFPPFNQMDTAHLAYLV
                     ENCQLRFYGEGETIIKPTDGPVEHFFIVKQGRVHGERPHSARRGTETTFEITTGECFP
                     LAALIGERATRTEHLAAEDTFCLLLNKPAFIKLFSLSNPLRDFALRGVSSLLDQVNQQ
                     VQMRAVETLGAQYSLDTRLGQLAMRNPISCGPEMPMRDAVQLMHEQQVGSIVIVDPAQ
                     KPLGIFTLRDLRRVVADGVDLTQPIDRLMTQSPFHLPPDASAFDAAMAMTERHIAHVC
                     LVEHGRLCGVISERDLFSLQRVDLVHLARTIRHAGRVETLAALRSDVRQLVDSMLAHG
                     ASSTQITQLITLLNDHTVCRVIELTLEDLGDPGVPFTWLVFGSEGRSEQTLYTDQDNG
                     ILFEANDAAEAAAIRGRLLPLAHEINLRLAQCDFTLCKGNIMAGNPELCLSRQEWSRR
                     FSSFVQEATPENLLASSIYFDLRAVWGEIEGCEQLRQGLLQQVAGNSLFQRMMAENAL
                     RQRPPVGVFRDFVVARTGAEKDTLDLKVQGLTPFVDGARLLALAHGVDACNTLERLRA
                     LIQQGVIEAQDGAAYEEAYHYIQQARMQQHQLQARSGLPYSNRVDPDNLNHLDRRILR
                     ESFRQAQRLQSSLAVRYQL"
     gene            complement(3054..5231)
                     /locus_tag="VFF22_08085"
     CDS             complement(3054..5231)
                     /locus_tag="VFF22_08085"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HZX16804.1"
                     /translation="MTERVQVGSLQVAKVLFDFVNNEAIPGTGIAADKFWAGAEAVIN
                     DLAPKNRALLAKRDAIQAQIDAWHQARAGQAHDAAAYKTFLQEIGYLLPEPADFQATT
                     QNVDEEIARLAGPQLVVPVMNARFALNASNARWGSLYDALYGTDVISEEGGAEKGRGY
                     NKVRGDKVIAFARAFLDEAAPLAAGSHVDSTGYRIEAGKLVVSLKGGSNSGLRDDAQL
                     VGFQGASDAPIAVLLKHNGLHFEIQIDANTPVGQTDAAGVKDVLMEAALTTIMDCEDS
                     VAAVDADDKVVIYKNWLGLMKGDLAEEVVKGGQTFTRTMNPDRVYTKTDGSELTLHGR
                     SLLFVRNVGHLMTNPAILDAQGNEIPEGIQDALFTSLIAIHNLNGNTSRKNTRTGSVY
                     IVKPKMHGPEEVAFTTEIFGRVEDVLGLTRNTLKVGIMDEERRTTVNLKACIKAASER
                     VVFINTGFLDRTGDEIHTSMEAGAVVRKAAMKSEKWISAYENWNVDIGLATGLQGRAQ
                     IGKGMWAMPDLMAGMLEQKIAHPLAGANTAWVPSPTAAALHALHYHKVDVFARQAELA
                     KRERASIDDILSIPLAKDTNWSADEIQNELDNNSQGILGYVVRWIDQGVGCSKVPDIN
                     DVGLMEDRATLRISSQLLANWLRHGIATEEQIVASLKRMAPVVDRQNAADPLYRPLAP
                     NFDSNIAFQAALELVVEGAKQPNGYTEPVLHRRRREFKAANGL"
BASE COUNT          891 a         1531 c         1764 g         1097 t
ORIGIN      
        1 agggcccgtt ccagcgaggg tagggcttta acagcctgtt gcttgggcta ttgccactga
       61 gtgcgagaag gcctggagcc gtagggtgcg ccgtgcgcac caatattggt tgccgcctct
      121 gctggacgga ctcaggcctg aatctctcta ttcaactcaa cccactattg gggcagtgcc
      181 gtttatatcg agtgggcgat ctggcggcgg cggctgttga gctgctgcag cagcgccggc
      241 aggttatcga gctgctgctg gcgcgccttg ttgaacagga tcagcgctag ctcggcggtg
      301 actagggcgt cggcgctggc gtggtgacgc tgctgcactt gcaggccgaa atgctcggtc
      361 cagtcgtcca acccggcatg gggcagattg gctttaggac aaaccagcgg ggcgatttcg
      421 gcgacatcaa agaaggcatg gcgcaagcgg tagcccaggc tctgcttgag tgcgcgggcg
      481 agcatgcgct ggtcgaactc ggcgtggaag gccagcagcg ggctgtcgcc gacaaactcc
      541 ataaagctca gcagggcttc ggccgggtcc tcgccattgg cgatctcgtt gggcgcgatg
      601 ccatggatca gggtgctggc gctggcctga tggccgtcgc gttgcagggt gcattcgaat
      661 tgctggctaa ggtcgatggc gccgttttcg ataaccaccg cgccaatcga cagcacctga
      721 tcgcggcgca tatttagccc gctggtttcc aggtcgagga tgacgaagcg ctgctggtgc
      781 agtggacgtt catcgaatgc gccgggttct gctagtttgc cgcgccgctc caattgcaat
      841 tgggtcagcg ccgggccacg cccgctaaac caggtgaact tgctcatagc tggtacctca
      901 cagccaggct gctctgtagc cgctgcgcct ggcggaaaga ttcacgcagg atgcgccggt
      961 cgaggtggtt gagattatcg ggatcgaccc gattggagta gggcaggcca ctgcgtgcct
     1021 gcaactggtg ctgttgcatg cgcgcctgct gaatgtagtg gtaggcctct tcataggccg
     1081 cgccatcttg agcttcgatc accccttgct gaatcagcgc gcgcaggcgc tccagggtgt
     1141 tgcaggcgtc cacaccatgg gccagagcga gcagacgggc accgtcaaca aagggcgtca
     1201 ggccttgcac tttgaggtcc agagtgtcct tttcggcacc tgtgcgggcc actacaaaat
     1261 cgcgaaaaac cccaaccggc ggccgctggc gtagggcgtt ctccgccatc atgcgctgaa
     1321 acaggctatt gccggccacc tgctggagta gaccctggcg cagctgctcg cagccttcga
     1381 tctcgcccca cacggcgcgc aggtcgaaat agatgcttga ggccagcagg ttctccgggg
     1441 tggcttcctg gacaaagctg ctaaagcgtc gtgaccactc ctggcgcgac aggcacagct
     1501 cagggttgcc ggccatgata ttgcccttgc acagggtaaa gtcgcactgc gccaggcgca
     1561 ggttgatctc atgggccagt ggcagcaggc ggccacgaat ggcggcggcc tcggctgcat
     1621 cattggcttc aaagaggatg ccgttgtcct gatcggtgta cagcgtctgc tcgctgcggc
     1681 cctcactgcc gaacaccagc caggtaaacg gcacgcccgg gtcgcccagg tcttccaggg
     1741 tcagctcgat cactcggcaa acggtgtggt cgttgagcag ggtgatcagc tgggtgatct
     1801 gcgtcgagct ggcgccgtgg gcgagcatgc tgtcgaccag ctggcgtaca tcgctgcgca
     1861 gcgccgccag ggtttctacc cgtccggcgt gacgaatggt gcgcgccagg tgtactaggt
     1921 cgacccgttg caggctgaac agatcccgct cggaaatcac tccgcacagg cgtccatgct
     1981 cgaccaggca gacgtgggcg atatgccgct cggtcatggc catcgccgca tcgaaggcgc
     2041 tggcatccgg cggcaggtgg aagggcgact gggtcatcag acggtcgatg ggctgggtca
     2101 ggtcgacgcc atcggccact acgcggcgca ggtcacgcag ggtgaaaatg cccagcggtt
     2161 tctgcgccgg gtcgacaatg acgatgctac cgacctgctg ctcgtgcatc agctgcaccg
     2221 cgtcacgcat cggcatttcc gggccgcagc tgatcgggtt gcgcatggcc agctggccga
     2281 gacgggtgtc gagggaatac tgcgcgccga gggtttccac cgcgcgcatc tgcacttgct
     2341 ggttgacctg atcgagcagg ctgctgaccc cgcgcagggc gaagtcgcgc agcgggttgg
     2401 acagcgagaa cagcttgata aaggccggct tgttcagcag caggcaaaag gtgtcttcgg
     2461 cggccaggtg ttcggtacgg gtggcgcgct cgccgatcag tgcggccagc gggaagcatt
     2521 cgccggtggt gatctcgaag gtggtttccg tgccgcggcg tgccgagtgc gggcgctcgc
     2581 catgcacgcg gccctgtttg acgataaaga agtgctctac cgggccgtcg gtcggcttga
     2641 tgatggtttc accttcgcca taaaaccgca gttgacagtt ctccaccaga tacgccaggt
     2701 gcgcggtatc catctggttg aacggcggga atttctgcag aaaggccatg gtgccatgga
     2761 tgttctgcag caccgccgtc ttgcctgcct gggcgattgc gtctgcgctg ctcatgggcg
     2821 gcctcgggag ttggctgtat aggaataatc ggccgcttct gtcatctggg ccattggaca
     2881 taagtctagc cgcgggcgca gcacgatttt gtagggtgtg ctgcgcgcac cagcaatttg
     2941 ctgccggagt tggtgcgcac agcgcaccct aagccttgtg gccatttgct atggggttat
     3001 caccataaaa aaaccgcccc caagggcggt tttttaagtg ctacagctgc ggtttacagg
     3061 ccgttggccg ctttgaactc gcggcggcgg cggtgcagga ctggctcggt gtagccgttc
     3121 ggctgcttgg cgccttcgac caccagctcc agggcagcct ggaaggcgat gttgctgtcg
     3181 aagttgggtg ccagcggacg gtacagcggg tcagctgcgt tctgacggtc aaccaccggg
     3241 gccatgcgct tgaggctggc aacgatctgc tcttcagtgg cgatgccatg acgcagccag
     3301 ttggccagca gctggctgga aatacgcagg gtagcgcggt cttccatcag gccgacgtca
     3361 ttgatatccg gcaccttgga gcagccaacg ccctggtcga tccagcgcac tacataaccg
     3421 aggatgccct gggagttgtt gtccagttcg ttctggatct cgtcagccga ccagttggtg
     3481 tccttggcca gcggaatgct caggatgtcg tcgatggacg cgcgctcacg cttggccagt
     3541 tcggcctggc gggcgaacac gtcgaccttg tggtagtgca gcgcgtgcag cgcggcggcg
     3601 gtcggcgacg gaacccaggc ggtgttggcg ccggccagtg ggtgagcgat cttctgctca
     3661 agcatgccgg ccatcaggtc gggcatggcc cacatgcctt taccgatctg tgcgcgacct
     3721 tgcaggccgg tggccaggcc gatatcgacg ttccagttct cgtaggcgct gatccacttc
     3781 tcggacttca tggcggcttt acgcaccacg gcgccagctt ccatggaagt gtggatttcg
     3841 tcaccggtac ggtcgaggaa gccggtgttg atgaatacca cgcgctcgct ggcggccttg
     3901 atgcaggctt tcaggttgac ggtggtgcgg cgttcttcgt ccatgatgcc gactttcagg
     3961 gtgttgcgag tcaggcccag gacgtcttca acgcgaccga agatttcggt ggtgaaggcg
     4021 acttcttctg gaccgtgcat cttcggctta acgatgtaga ccgaaccggt gcgggtgttc
     4081 ttgcggctgg tgttgccatt caggttgtgg atggcgatca ggctggtgaa cagcgcgtcc
     4141 tggatgcctt ctgggatctc gttgccttgg gcatcgagga tcgccgggtt ggtcatcagg
     4201 tggccaacgt tgcgcacgaa tagcaggctg cgaccgtgca gggtcagctc gctaccgtcg
     4261 gttttggtgt agacgcgatc cgggttcatg gtgcgggtga aggtctggcc gcctttgacc
     4321 acttcttccg ccaggtcgcc cttcatcagg ccgagccagt tcttgtagat caccactttg
     4381 tcgtcagcat cgacggccgc tacggagtct tcgcagtcca tgatggtggt cagcgcggct
     4441 tccatcagca cgtctttaac gccggcagca tcggtctggc cgaccggggt gttggcgtcg
     4501 atctgaattt cgaaatgcag gccgttgtgc ttgagcagta cggcgatagg cgcgtcgctt
     4561 gcgccctgga aaccaaccag ttgtgcgtcg tcacgcaggc cgctgttgct gccgccttta
     4621 aggctgacaa ccagcttgcc ggcttcgatg cggtaaccgg tggagtcgac gtgggagcca
     4681 gcagccagcg gcgcggcttc gtcgaggaag gcgcgggcga aggcgatgac cttgtcgccg
     4741 cgaaccttgt tgtagccacg gcctttttcg gcgccgcctt cttcgctgat cacgtcggtg
     4801 ccgtacaggg cgtcgtacag cgagccccag cgggcgttgg aggcgttgag ggcgaagcgc
     4861 gcgttcatca ccggcacgac cagctgtggg ccggccagac gggcaatttc ttcgtcgacg
     4921 ttctgcgtgg tggcctggaa gtcagccggc tctggcagca ggtagccgat ttcctgaagg
     4981 aaggttttgt aggcagcggc gtcatgggcc tggcctgcgc gggcctggtg ccaggcgtcg
     5041 atctgcgcct gaatcgcatc gcgtttggcg agcagcgcac ggtttttcgg ggccaggtca
     5101 ttgatgaccg cttcggcacc cgcccagaac ttatcggcag caatgccggt accggggatg
     5161 gcttcgttat tcacgaagtc gaacagcact ttggcaacct gcaggctgcc tacttgaacg
     5221 cgttcagtca tcacttgcct cactctgctc agctctactc aatagccgcg tcggacatgg
     5281 gaa
//
LOCUS       DASRUM010000120         4102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1072394, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000120 DASRUM010000000
VERSION     DASRUM010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072394"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..748
                     /locus_tag="VFF22_08090"
                     /pseudo
     CDS             1..748
                     /locus_tag="VFF22_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021138877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
     gene            complement(880..1092)
                     /locus_tag="VFF22_08095"
     CDS             complement(880..1092)
                     /locus_tag="VFF22_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16805.1"
                     /translation="MKLYHPPSGITQGLELLIDPNWSPDQAMAVIELIDDLRDRIWSH
                     YEIVLQQKFRDERITEYDVEISDPPF"
     gene            complement(1089..1847)
                     /gene="istB"
                     /locus_tag="VFF22_08100"
     CDS             complement(1089..1847)
                     /gene="istB"
                     /locus_tag="VFF22_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZX16806.1"
                     /translation="MTTSDARRKRAMALQLHGVLAHWSECADKAWLEPLLAWEESERS
                     RRSLERRLRCAHIGRFKPLADFDWAWPEQCDQAAIAELMTLDFLHSATNAILVGPSGL
                     GKTTIAQNIAHQATLQGHTVLFTTAGQLLGELASLDSDSALRYRLRRYAAPSLLVVDE
                     VGYLSYSNRHADLLFELISRRHEKKSTLITTNKSFGEWSEVFPNAACVVALIDRLVHH
                     AEIIPIKGESYRHKEAQERAAAKAKKRKGARSKS"
     gene            complement(1834..3333)
                     /gene="istA"
                     /locus_tag="VFF22_08105"
     CDS             complement(1834..3333)
                     /gene="istA"
                     /locus_tag="VFF22_08105"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZX16807.1"
                     /translation="MALTPEIEAQILRYYHAERWRVGTIATQLQLHRDTVARVLSQAG
                     LPAIGPVRRSSAIDPYLPFIRETLEQFPTLTASRLYGMVQSRGYPGRPDHFRHLIARH
                     RPRRRTEAYLRLRTLPGEQCQIDWGHFGHIEIGRARRPLMGFVTVLSWSRQIFLRFYL
                     DARMENFLRGHVAAFEAWGGVPRIALYDNLKSAVLERQGNAIRFNPTLLALAGHYRFE
                     PRPVAVARGNEKGRVERAIRYIRDAFFAGRTFTDLDDLNAQADAWTSGPASERRCPED
                     VKLTVKATFARERERLLALPPNAFATDEVKAVSVGKTPYVRFDLNDYSVPHAHVQRTL
                     TVVADPCRVRLLDGAAMLATHPRSYDRGQQIEIQGHIDTLVEHKHQASAHRITDRLVQ
                     AVPHSRELLRQAAERGEPLGRVARTLLDLLDRYGAAELEIAIRDALAREVPHPNAVRL
                     ALERRRQEQDSPPPIGVTLPDHVKRRDVTVRPHPLAGYDQLTEAGHDDE"
BASE COUNT          781 a         1290 c         1343 g          688 t
ORIGIN      
        1 cgaaagctgg gatgagtacg ggcagcggga gcagacccgg cgcgagcacc tggtcgagct
       61 gcaaacggtg tttggcttcc agccgttcac catgagccac taccggcagg ccgtgcacac
      121 gctgaccgac ctggccatgc aaaccgacaa aggcatcgtg ctggctagca ccttggttga
      181 gcatctgcgg cgacagtcga tcatcctgcc cgcgctcaac gccatcgagc gcgctagcgc
      241 cgaggccatc actcgcgcca accggcgcat ctatgacgcc ttggccgaac cactgtcgga
      301 cacgcatcgc cgccgcctcg acgatctgct caggcgccgg gacaacggca agacgacctg
      361 gctggcctgg ctgcgccagt cgccggttaa accgaactcg cggcacatgc tggaacacat
      421 cgaacgcctc aaggcgtggc aggcactcga cctgccttct ggcatcgagc ggctggttca
      481 ccagaaccgg ctgctcaaga tcgcccgcga gggtggccag atgacgcccg ccgacctggc
      541 caagttcgag ccacagcggc gctacgcgac cctgatggcg ctcgccatcg agggcatggc
      601 caccgtcacc gacgaaatca tcgacctgca tgaccgcatt ctcggcaagc tgttcaacgc
      661 cgccaagaac aagcatcagc agcagttcca ggcatccggc aaggcgatca acgccaaggt
      721 gcgactgtac ggacgcatcg gccaggcgtg ttagcggtgc tgtcgccacg cacttagccg
      781 gcgtcgattg ttggcggcgg aatttttcgt tggcgtaatg gtgacgggta aacgaaacag
      841 gggccggcag gcccctgaag aagagaagat cgcgggtggt cagaaaggcg gatcggagat
      901 ctcgacgtcg tactccgtga tgcgctcgtc gcgaaacttc tgctgtagga cgatctcata
      961 gtgagaccag atccgatcgc gcagatcatc gatcagttcg atcacggcca tggcctgatc
     1021 gggcgaccag ttcggatcga tcagcagttc aaggccctgc gtgatgccgg aaggtgggtg
     1081 gtagagtttc atgacttgct ccgggcgccc ttgcgcttct tggccttggc cgcggcacgc
     1141 tcctgagcct ccttgtggcg ataggactca cccttgatcg gaatgatctc ggcgtgatgc
     1201 accaggcggt cgatgagcgc gaccacgcaa gccgcattgg gaaacacctc cgaccattcg
     1261 ccgaaggact tgttggtggt gatcagcgtg ctcttcttct cgtggcgccg actgatcaac
     1321 tcgaagagca gatccgcatg gcggttcgaa taagacaggt agcccacctc gtcgaccacc
     1381 agaagagacg gtgcggcata gcggcgcaag cgatagcgca aggccgaatc gctgtcgagc
     1441 gacgccagtt cgccgagcaa ctggccggcg gtggtgaaca gaaccgtatg cccctgcaac
     1501 gtcgcctgat gcgcgatgtt ctgggcgatg gtcgtcttgc ccagaccgga gggtccgacc
     1561 aggatggcat tggtggccga gtgcaggaag tccagggtca tcaactccgc gatggcggcc
     1621 tgatcacatt gctcgggcca ggcccaatcg aagtccgcca gcggcttgaa gcgaccgata
     1681 tgcgcacagc gcaggcggcg ctccagggaa cgccgggaac gctcggactc ctcccacgcc
     1741 agcaagggct ccagccaggc cttgtcggca cactccgacc aatgcgccag cacgccatgc
     1801 agttgcaaag ccatggcccg ctttcgacga gcatcactcg tcgtcatggc cggcctccgt
     1861 cagttgatcg taacccgcca aaggatgcgg ccgaaccgta acatcgcgcc gcttgacgtg
     1921 atcgggcaag gtcacgccaa tggggggcgg tgaatcctgt tcctgacgcc ggcgttccag
     1981 cgccagacgc accgcgttcg gatgaggcac ctcgcgcgcc agcgcgtcgc gaatcgcgat
     2041 ctccaactct gcggcgccat agcgatcaag gagatcaagc agcgtgcgcg cgacgcgccc
     2101 cagcggttcg ccgcgttcag ccgcctggcg caacaactcc cggctgtgcg ggaccgcctg
     2161 caccagacga tcggtgatgc gatgtgcact cgcctggtgc ttgtgctcga ccaaggtgtc
     2221 gatatgcccc tgaatttcaa tctgttgccc acggtcatac gagcgcggat gtgtcgcgag
     2281 catcgccgcc ccatcgagga ggcgcacccg gcacggatcg gcgaccacgg tcagtgtgcg
     2341 ctgaacatga gcatgcggca ccgagtagtc gttcagatcg aagcgcacat agggggtctt
     2401 gccgacggac acggccttca cctcgtcggt ggcaaaggcg ttgggcggca aggccagcaa
     2461 acgttccctc tcccgcgcaa aggtcgcctt caccgtcagt ttcacgtcct cggggcagcg
     2521 gcgctcgctg gcagggccgc ttgtccaggc gtcggcctgg gcattcaggt cgtcaagatc
     2581 agtgaaggtg cgcccggcga agaaggcatc gcggatgtag cggattgccc gctctaccct
     2641 ccctttctca tttcctcgcg cgacggccac cgggcgtggc tcgaaccggt aatgcccggc
     2701 cagtgccaag agcgtcgggt tgaaacggat ggcgttgccc tgccgttcca gcacggcact
     2761 tttaagattg tcatagagag cgatgcgggg aacgccgccc cacgcctcaa aggcggcgac
     2821 atggccgcgc aggaagttct ccatgcgtgc gtccaggtaa aagcgcagga agatctgccg
     2881 tgaccaggac aggaccgtga cgaaacccat caggggtcgg cgggcacggc cgatctcgat
     2941 gtgaccgaag tgaccccaat cgatctggca ttgttcaccc ggcaaggtgc gtagccgcag
     3001 ataggcttcg gtccgccgac gcggccgatg gcgggcgatc agatgccgaa agtgatccgg
     3061 gcggccgggg tatccgcggc tttgcaccat gccgtacagg cgactggccg tgagggtcgg
     3121 gaactgctcc agcgtttcgc ggatgaacgg cagataggga tcgatggccg aggaacggcg
     3181 caccggtccg atcgccggca agccggcctg ggacaggacg cgggcgacgg tgtcgcggtg
     3241 cagttgcagt tgggtggcga tggtgccgac gcgccagcgt tcggcatggt agtagcgcag
     3301 gatctgcgct tcaatttcgg gggtgagggc cacgggcaat tctcctgtcg gaatggaatc
     3361 ggcgaaccga ggagcgcaga aagtcctgac gcacgaattc cgggagtgcc cgatggcaca
     3421 gggtgcagtg ccccggcggc ggcatgggtt tcgtcgctgt cgattcggcc gccagttgaa
     3481 caggcaggga ccggggtggg gaaccgtgat gcgtcacttt ctgttgccca gcccggtaac
     3541 ggcgctgccg ttcggcgtgg gcgtgacgac cgcgcaagct gtcttgatag cgacggccgg
     3601 cggctcgaac cgcacagcgg cgggcctgct cggcacaccc cgcggtgcag tagcgatggc
     3661 cgcgatcaca gtggctgcaa atgagcacag gggtgcggca gccggcgcat aggtagcggc
     3721 gtcctggagg ttccattggc aagcagggcc aatgggggac tcgacgggcg cggccgcttc
     3781 gtgaaatact tcgcgtgcaa cgtcgcccgc cagggcgcgg ggcgcgacat cggaactggc
     3841 ttggcggttg ggaccggtgt cgcgcctgcg tttccaccgg ccggtcggct ttctacctca
     3901 tcagcgacgc cggcgctaca tccacgcagg ccagacgggt tcttcgttag acagcttcag
     3961 cctaccctgc cgaccccgtc tcgcgatccc tgccgcgctt cgcttgccag acaacgattg
     4021 ctgcgcaatc catccgccgg aagccctgcg ggcagcaaac cgtaaaagcg gcatagtcat
     4081 gcctgaaatt caaaacccaa aa
//
LOCUS       DASRUM010000121         8147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1411188, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000121 DASRUM010000000
VERSION     DASRUM010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8147
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411188"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VFF22_08110"
     CDS             <1..760
                     /locus_tag="VFF22_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HZX16808.1"
                     /translation="TRFGWVQSYGSRCVKPAVIYGDLSRPKAMTVAWIGYAQTLTRKV
                     MKGMLTGPVTMLMWSFPRDDISREQQARQLALAIRDEVVDLETAGIKIIQIDEAAFRE
                     GLPLRQAQWQAYLNWATEAFRLCASGVRDETQIHTHMCYSEFNDVIESIAAMDADVIT
                     IETSRSDMELLEAFERFDYPNDIGPGVYDIHSPRVPDSSEMVKLLLKAAQRIPAERLW
                     VNPDCGLKTRAWPETEAALINMVSAARQLRTELV"
     gene            969..1988
                     /locus_tag="VFF22_08115"
     CDS             969..1988
                     /locus_tag="VFF22_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011268732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX16809.1"
                     /translation="MRGLILLAALLCGLPVFAAQRCDVEVPTHTAAVGDVRLAYQSIG
                     RDSDPALLLVMGLGGQLIHWPDEVVARLCQQGFRVIRFDNRDVGLSTWAGKAPDINLT
                     YEALRYRLGLSVAAPYGLRDMAKDALGLLDALAVREVHVLGASMGGMIAQHMADLAPE
                     RVQSLTLIMTSSGAQGLPAPSSALLSLLAKREAPNRAIALEQQADLLAALGSPTVSDD
                     RQLLLQQAQIAYDRAFNPEGVQRQLLAILAEPSRVELLNRLRVPTLVVHGTADPLLPV
                     MHGVHVAAHIKNSELKLIPGLAHRFQEAFKEPLLAAVLPHLKAQQLNGPQLARLPAAE
                     LSYIQ"
     gene            complement(1933..2670)
                     /locus_tag="VFF22_08120"
     CDS             complement(1933..2670)
                     /locus_tag="VFF22_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX16810.1"
                     /translation="MQTAQFDLRAALPNVMPTAAPMRIYLEGDGRAWVTAQQPSLDPT
                     PRDQLVAQLALEDPQPSIYLARPCQYLSSPSCTPRYWTDARFAEEVLASLDQALDQLK
                     QRYGNSDFELIGYSGGGTLALLLATRRNDIAQVQTLAGNLSPRRWAELLDLTPLHNSL
                     EPLDFAERLQAVPQRHLAGRDDQTVLASLLNEYAARLGSADCLELHLIDGVSHHAGWR
                     EHWPGWRDQPIECRKARRPASEQVEAR"
     gene            complement(2810..5557)
                     /locus_tag="VFF22_08125"
     CDS             complement(2810..5557)
                     /locus_tag="VFF22_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HZX16811.1"
                     /translation="MARPYLRKTLLALAIASHQQLLADTLDLTGSQATLYSNEYLAPL
                     TITGQASGAGYAVDVNSWFFHFFPSITNQANLNSFGEETKTLSLSGSGTFDYFWGLDV
                     IAGDLTNEGQITANGDSSSGIFLGNTAAILLGDLNNSGNITSDGTNSAGIRLSNLATI
                     FGGGINNTGNISANGAGSSGISLESNSVTYSLHNSGAIRVNGTAAKGIRLSEAWIQEH
                     LQNSGNIEVSGESSVGIALENDSVVSAMGNTETGVISASGDYSRAISIAQGKLSLSPT
                     SLQNRGLIQTIGRESVGVYIDHPSNLSILINEGQISSQGVGSRAIALNSDYPDAPASS
                     EGLWVINTGTLQAEGIAIETAPSPANYDGFGSWLQVDMWSGLISGGDAAIKGDGNVLL
                     NFTGGEIRGNLLGLQTIYASGDALFNGQLIQSTDLWVSALELAQPHTRLDGNLNLGDS
                     TLDLNLHNQTDSSRAIFDVSGSATFGLFGQSHIRLKPASEDFRGLPLREYVLISANSI
                     EDQGTPFGISLQVTSLSDLLQIESFEVTDNQVTAVVKGLSAKQVSDYLRENGAPAYVL
                     PAFTRFYGKTLGNLSDADPLFQSVISSNDAELVRLAQQLTPEVDGAASQVAASHHNLL
                     GNALQNRSASLRRGLSSGDGLSQTGAWVQVLDSDADQDSGNGIPGYDADSQGIAMGAD
                     GKLNAQTTLGLAYSYLSSDVRSQSGNKTDIEGHTLTLYSTYEQDAWFADASLSYGLSD
                     NQSKRYIAGTRARGDYDSSLWGLNLLGGYGFDLSQGVLLEPRIAARYSNVQIDSYREK
                     GSSAALAVGSQRIEIAELGTGVRLAGNFAVGQGNLEPEAKLMAYHDFIADQASSTSAF
                     LAGGTPFVTNGAKPARDSYEASLGLTYRLGAVSLGLSYDYLTKADYTADTVQARVRYD
                     F"
     gene            complement(5816..6322)
                     /locus_tag="VFF22_08130"
     CDS             complement(5816..6322)
                     /locus_tag="VFF22_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HZX16812.1"
                     /translation="MSLSMHQASVEVFTRQLNNLSTILSIAAAHAEEKKIEQSVFLNA
                     RLAPDMFPLNRQVQIACDTAKAGAALLAEVEAPNHADDETSFAELQERIAKTLSFLQS
                     LSAAQIDGSEARTVTLKRRDKETHFQGQAFLLDHVLPNLYFHITTAYAILRHNGVAIG
                     KRDFLGTR"
     gene            complement(6422..6892)
                     /locus_tag="VFF22_08135"
     CDS             complement(6422..6892)
                     /locus_tag="VFF22_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017702716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZX16813.1"
                     /translation="MTQSSNTPYDVLEVGQTASYSKTVSERDIQLFAEVSGDHNPVHL
                     DAEYAATTMFKERIAHGMFSGALISAAVACELPGPGTIYIGQSMRFTAPVKLGDTLTV
                     RLEILEKLPKFRVRVATRVFNQNDELVVDGEAEILAPRKAQTVELTKLPPITIG"
     gene            complement(6889..7851)
                     /locus_tag="VFF22_08140"
     CDS             complement(6889..7851)
                     /locus_tag="VFF22_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX16814.1"
                     /translation="MLHQAFWLNSTDAAPLFVNRWYAEQPPKALLMVAHGMAEHSGRY
                     ARLAEALVAQGFEVYAHDQRGHGKTAEHGLLGHFADSKGWELVVNDLAHLNHHIRQIH
                     PQTPIFLLGHSMGSYIGQAYLMQHSCSLQGAILSGSNYQPVALYRIGQLLARFERWRQ
                     GPHAYSALLEFASFGSFNKAFKPNRTAFDWLSRDPLEVDKYTNDPLCGFRCSNQLWVD
                     LLGGLQQITPLQSLAQIDNSLPLLVIGGACDPVSDGHRQQDLGNALQAAGNPHVQIKI
                     YPEARHELLNESNRDEVTAYIIDWLQQALSQPRQRHLQSQESHP"
BASE COUNT         1520 a         2244 c         2578 g         1805 t
ORIGIN      
        1 tacccgtttc ggttgggtgc agagctacgg ttcgcgttgc gtaaaaccgg cagtgatcta
       61 cggcgacctg agtcggccca aggccatgac tgtggcctgg atcggttatg cgcagaccct
      121 tacccgcaaa gtgatgaagg gtatgctgac cggcccggtg accatgctga tgtggtcctt
      181 cccgcgcgac gacatttccc gtgaacagca ggcgcgtcag ctggccctgg cgattcgtga
      241 cgaggtggtt gacctggaaa cagcgggtat taagatcatc cagatcgacg aagccgcctt
      301 ccgcgaaggc ctaccgctgc gccaggcgca gtggcaggct tatctgaact gggccaccga
      361 ggcattccgt ctgtgcgcct cgggcgtgcg tgacgaaacg cagatccaca cccacatgtg
      421 ctacagcgag ttcaacgatg tgatcgagtc catcgccgcc atggatgccg acgtgatcac
      481 catcgagacc tcgcgttcgg atatggagct gctggaggcc ttcgagcgct tcgactatcc
      541 caacgacatt ggccccggcg tgtatgacat tcactcgcca cgggtgccgg acagcagcga
      601 aatggtcaaa ttgctgctca aggccgccca gcgcatcccc gctgaacgtc tgtgggtcaa
      661 cccggactgc ggtctgaaaa cccgcgcctg gccggagact gaggcggcgt tgatcaatat
      721 ggtgtctgcc gcacggcagc tgcgcaccga gctggtctga tcagcgtact gtcgcggcta
      781 aagcccctcc cacggttttg atcagctcta gcaggggctt tagttggcgc ttcttgtcgt
      841 tatagggcga ccgctcgtct cccttcatca aactgtcacg cttgtgtggc agcgcgctcg
      901 cacaaacatc accacactcg cgtcttaatg gggtgctgcg ttctgctgtc ccggatctgg
      961 gtgatgttat gcgtgggttg attcttctcg ctgcactgtt gtgcggcctg ccggtttttg
     1021 cggcgcagcg ttgtgatgtg gaggtgccga cgcatacagc ggctgtgggc gatgtgcgtc
     1081 tggcgtacca gagcattggc cgcgacagtg atccggcgct gctgctggtg atgggcctgg
     1141 gcgggcagct gatccactgg ccggatgaag tggtggcgcg cctgtgtcag caaggctttc
     1201 gggtgatccg cttcgataac cgcgatgtcg gcctgagcac ttgggccggc aaggcgccag
     1261 acatcaatct gacctatgag gcgctgcgtt atcggctcgg tctgtcagtg gccgcgccct
     1321 acgggctgcg cgatatggcc aaggatgccc tgggcctgct ggatgcattg gccgtgcgtg
     1381 aggttcacgt gctcggcgcg agcatgggcg gaatgatcgc ccagcatatg gccgaccttg
     1441 cacctgagcg ggtgcagagc ctgaccttga tcatgaccag ctccggtgcc caggggttgc
     1501 cggcgccaag cagcgcgctg ctgagtctgc tggccaaacg tgaagcgccc aatcgcgcga
     1561 tagccctgga gcagcaggcc gacctactgg cggcgctggg cagcccaacg gtcagcgatg
     1621 atcggcagtt attgctgcaa caggcgcaaa tcgcctatga ccgtgcgttc aacccggaag
     1681 gcgtgcaacg gcagttgttg gcgattctgg ctgaaccgag tcgggtcgaa ctgctcaacc
     1741 gcctgcgtgt gccaaccctg gtggtgcatg gcacggccga tccgctgctg ccggtgatgc
     1801 acggtgtgca tgtggcggcg catatcaaga acagcgaatt gaaactgatt ccgggtcttg
     1861 cccatcgctt tcaggaagcg tttaaggaac cgctgctggc agcagtgttg ccgcacctca
     1921 aggctcagca actcaacggg cctcaacttg ctcgcttgcc ggccgccgag ctttcctaca
     1981 ttcaataggc tggtcgcgcc aacctggcca gtgctcgcgc cagccggcgt gatgactgac
     2041 gccatcgatc aggtgcagtt ccaggcagtc ggctgaaccc aggcgcgctg cgtattcgtt
     2101 caacaagctg gctagcactg tttggtcgtc gcggccggcc agatgccgtt gcggtactgc
     2161 ttgcagacgt tcggcaaagt ccagcggctc cagcgagttg tgcagcggcg ttagatcgag
     2221 taattcggcc cagcgacgcg ggctcaggtt gccggccagg gtttgtacct gggcgatatc
     2281 gttgcgccgg gttgccagca acaatgccag ggtgccgccg cccgaataac cgatcagttc
     2341 gaagtcgctg ttgccatagc gttgtttgag ttgatccagg gcctggtcaa ggctggcgag
     2401 cacctcttca gcgaagcgtg catcggtcca gtagcgcggt gtgcagcttg gcgagctcag
     2461 gtattggcag gggcgggcca ggtagatgct gggctgcgga tcttccaggg ccaattgcgc
     2521 caccagttga tcgcgcggtg tcggatccag gctgggttgc tgggcagtaa cccaggcgcg
     2581 gccatcaccc tccagataga tccgcattgg cgcagcggtt ggcatgacgt tgggcagggc
     2641 tgcgcgcaag tcgaactgag ctgtctgcaa ggtgatcagt cggtgcgcgt gttgagcggc
     2701 cagttcctcc aaacgagtgc tgggggatgt acaggcgctc aggagtatca gcaagcaaat
     2761 aaggctaacc ccggcccgca tgcgcggggc gggatagctg tcgtagggct tagaagtcgt
     2821 aacgaacccg agcctgaacc gtatcggcag tgtagtccgc cttggtcagg tagtcgtagc
     2881 tgaggcccag gctgactgcg ccgagacgat aggtcaggcc caggctggct tcgtaactgt
     2941 cgcgcgcagg tttcgcgccg ttggtcacga agggcgtacc accggccagg aaggcgctgg
     3001 tactgctggc ctggtcggcg atgaaatcgt ggtaagccat cagcttggct tccggttcca
     3061 gattgccctg gccgacagca aagttgccag ccaggcgcac gccggtaccc agctcggcga
     3121 tttcgatgcg ttgtgagcca actgctagcg cggctgatga gcccttctct cggtaactat
     3181 cgatctgcac attgctgtaa cgcgccgcga tgcgtggctc aagtagcacg ccttggctaa
     3241 ggtcgaagcc ataaccgccg agcaggttga ggccccacag cgagctgtca tagtcgcccc
     3301 tggcacgggt gccggcaata tagcgcttgc tctggttgtc gctaaggccg tagctcaggc
     3361 tggcgtcggc gaaccaggca tcttgttcgt aagtgctata gagggtcagg gtgtggcctt
     3421 cgatatcggt cttgttgccg ctttggctgc gtacgtcgct gctcaggtag ctgtacgcca
     3481 ggcccaaggt ggtttgcgca ttcaacttgc cgtcggcacc catagcaatg ccctggctgt
     3541 ctgcgtcgta gccgggaata ccgttgccgc tgtcctgatc ggcgtcgctg tccagtacct
     3601 gtacccaagc accggtctga ctcaaaccgt cgccagacga caggccccga cgcagacttg
     3661 cgctgcggtt ttgcagggcg ttgccgagca ggttgtgatg gctggcagca acctggctgg
     3721 ctgcgccgtc gacttcgggt gtgagctgct gggccagacg taccaattca gcgtcgttgc
     3781 tactgataac gctctggaac agcggatcgg cgtcgctcag gttgcccaaa gttttgccat
     3841 agaagcgggt gaaggccggc agtacataag ccggagcgcc attttcgcgc aggtagtcgc
     3901 tgacttgttt tgccgacagg cctttaacca ccgcagtgac ctggttgtcg gtgacttcga
     3961 aactttcaat ttgtagcaga tcggacaggc tggtgacttg caggcttata ccgaaaggtg
     4021 tgccttggtc ttcgatgctg ttggctgaga tcagcacata ttcacgcaga ggcaaaccac
     4081 ggaaatcttc cgaggccggc ttcaggcgga tatggctttg gccgaataaa ccaaacgttg
     4141 cactgccact gacgtcaaaa attgccctgc ttgaatctgt ctggttatgc aggtttaggt
     4201 caagagtgct atcgcccagg tttaaattgc cgtcaagacg ggtgtgcggc tgagccagtt
     4261 ctagtgctga cacccatagg tcagtgctct ggatcagttg gccattgaac agcgcatcgc
     4321 ccgaggcata aatggtttgc agccccagca gattgccccg aatctccccc ccagtgaagt
     4381 tcagcaaaac attgccatcg cctttgatgg ccgcgtctcc acccgaaatt aatcctgacc
     4441 acatatcgac ctgaagccag gagccgaaac cgtcgtaatt ggcaggggat ggcgctgttt
     4501 caatggcaat accttcggcc tgtagtgtgc cggtattaat cacccagagg ccttcagatg
     4561 atgcaggggc gtctgggtag tcgctgttga gggcgatggc tcggctgcca acgccttgcg
     4621 aggatatttg cccctcgttg atcagtatgg ataggttact tggatggtct atatagacgc
     4681 cgacgctttc acgaccaata gtttggataa gtccacggtt ttgaagcgat gttggagaaa
     4741 gagatagctt gccctgagca attgaaatag cgcggctgta atctccggag gcgctaatca
     4801 cgcctgtctc cgtattgccc attgccgata caacactgtc attctcaagc gcgatgccaa
     4861 cgctggattc gccgctgact tcgatgttgc cgctgttttg cagatgctcc tgaatccagg
     4921 cttcagacaa ccggattccc ttggctgccg tgccgttaac tcgaatggcc ccactattat
     4981 gcaggctata cgtcacgctg tttgactcaa gactgattcc ggatgacccg gcaccattgg
     5041 cgctaatgtt tccggtattg tttatacctc caccgaaaat tgtagctagg ttgctaagcc
     5101 taataccggc ggagttggtt ccgtcgctgg tgatattccc cgagttattc aagtcgccga
     5161 gcagtatggc tgccgtgttg ccgagaaaaa tcccgctaga gctgtctccg tttgcagtga
     5221 tctgcccttc attagtcaga tcgcctgcta ttacatccag cccccaaaag tagtcaaagg
     5281 tgcctgagcc gctcagggat aacgttttcg tttcttcgcc gaaggaattt agattggctt
     5341 ggttggtgat gctcggaaag aagtggaaaa accatgagtt cacatctacc gcgtatcccg
     5401 ctccagatgc ctgtccggta atagtcagag gggccaggta ttcgttggag tagagtgtcg
     5461 cctgtgaccc agtcagatca agggtgtctg ccagcaactg ctgatggctg gcaatcgcca
     5521 gggcaagaag ggtcttacgc aaatatgggc gggccatggt ctactccttg tcggccttta
     5581 aaggggcata agacgctttc aggcgaccga gaagatgttc gccagcttcc ttgctgagcg
     5641 catcttaatg gctaataggc atcatgcaac tgttaagtta tctgccaaga tcgcgtcgta
     5701 ggtaacaact gcatgactgc ttattaagac ggggtgtccc cctctgtgcg cagccgcccg
     5761 tgggcggtta tgcgctatta ccggagttac cccaggcttg gtgcaaacta ggggctcagc
     5821 gagtgccgag gaaatcgcgc ttaccaatag ccacgccgtt gtggcgcagg atggcgtagg
     5881 cggtggtgat gtggaagtac aggttcggca gcacgtgatc gagcaggaag gcttggccct
     5941 ggaagtgggt ttccttgtcg cggcgcttga gggttacggt gcgtgcttcg ctgccgtcga
     6001 tctgcgcggc gctcaggctt tgcagaaagc tcagggtctt ggcaatgcgt tcttgcagct
     6061 cggcgaagct ggtttcgtcg tcggcatggt tcggtgcttc cacttcagcc agcagtgcgg
     6121 caccggcttt ggcggtgtcg caggctatct gcacttggcg gtttagcggg aacatatcgg
     6181 gggctaggcg tgcattaaga aatacgcttt gttcgatctt cttttcttcg gcatgggcgg
     6241 cggcgatgct gagaatagtc gacaggttat tcagctgacg ggtgaatact tcgacggaag
     6301 cttggtgcat agacagggac atgaatatct ccagaagcgc cgaaacggat tgtctgggcg
     6361 caattacgta agtaggggtg tgcaccgccg caggtagccg cggtgacttg aatgcgcaag
     6421 cttagccgat ggtgatcggc ggcaatttgg tcagttcaac cgtctgtgct ttgcgcggag
     6481 cgaggatttc cgcttcgccg tccaccacca gctcgtcgtt ctggttgaac acgcgagtcg
     6541 ctacgcgtac gcggaatttc ggcagtttct cgagaatttc caggcgcacg gtgagggtgt
     6601 cgccgagttt taccggtgcg gtaaagcgca tgctctggcc aatgtagatg gtgcccgggc
     6661 caggcagctc gcaggccacg gcggcgctga tcagcgcacc gctgaacata ccgtgagcga
     6721 tgcgctcctt gaacatggtg gtggccgcgt attcggcatc caggtgcacc gggttgtgat
     6781 cgccggagac ttcggcaaac agctggatat cgcgttcgct gacggttttg ctgtagctgg
     6841 cggtctggcc gacttcaaga acgtcgtagg gcgtgttgct gctctgggtc atggatggct
     6901 ctcttgactc tgtaggtggc gctggcgcgg ttggctaagg gcctgttgca accagtcgat
     6961 gatgtaggcg gtcacttcgt cgcggttgct ttcgttgagc aactcgtgac gagcttccgg
     7021 atagattttt atctgcacat gcgggttgcc ggcggcttgc agggcattgc ccaggtcttg
     7081 ctgacgatgg ccgtcgctga ccgggtcaca ggcgccgcca atcaccagca gcggcaggct
     7141 gttgtcgatc tgcgcgaggc tttgcagtgg agtgatctgt tgcaggccgc cgagcaggtc
     7201 gacccacagc tggttgctgc agcggaaacc gcacagcggg tcgttggtgt atttgtcgac
     7261 ttccagcggg tcgcggctca gccagtcgaa ggccgtgcgg ttcggcttga acgctttgtt
     7321 gaacgagccg aatgaggcga attcgagcag agcgctgtat gcgtgcgggc cttgccgcca
     7381 gcgctcgaag cgcgccagca actggccgat gcggtacagc gccacgggct ggtagttgga
     7441 gccggaaagg atcgcgccct gcaggctgca gctgtgctgc atcaggtagg cctggccgat
     7501 gtagctgccc atgctgtggc cgagcaggaa aatcggcgtt tgcgggtgga tctggcgtat
     7561 atggtggtta aggtgggcca ggtcattgac cactagttcc cagcccttgc tgtcggcaaa
     7621 gtggccgagc aggccatgtt cggcggtttt gccgtgcccg cgctggtcat gcgcgtaaac
     7681 ctcaaagcct tgcgccacca gcgcttcagc caggcgcgca tagcgcccgc tgtgttcggc
     7741 catgccgtgg gcgaccatca gcaacgcctt gggcggctgt tcggcatacc agcgattgac
     7801 aaataggggc gctgcatcag tgctgttgag ccagaacgct tggtggagca tggcgaatcc
     7861 cttaagcctg cagaagctgc gcattgtgca ccccgcgcgg cgcggcgcaa agtccaccca
     7921 cgtgcagcgg atgcctgagg gcatggagag tcgcctgcgc tcggctggta tgtgccggcg
     7981 cgacaggata gttctacgcg gagcgacaag attcagtgcg tcaatgacgc tgctcggcta
     8041 tttgcctgtt tggcgttagc tgttaacttc cagccaactc cgcagcgcgg attagaccat
     8101 ctcagggcaa gaggataaaa ataatgcagc ctgatttctg gagcgac
//
LOCUS       DASRUM010000122         3287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1411237, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000122 DASRUM010000000
VERSION     DASRUM010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411237"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..2402
                     /locus_tag="VFF22_08145"
                     /pseudo
     CDS             1..2402
                     /locus_tag="VFF22_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251758.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
     gene            complement(2645..>3287)
                     /gene="rnt"
                     /locus_tag="VFF22_08150"
     CDS             complement(2645..>3287)
                     /gene="rnt"
                     /locus_tag="VFF22_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009618408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HZX16815.1"
                     /translation="ESSPSGPRHPMAARFRGYLPVVVDVETSGFNCATDALLEIATTT
                     IAMDEQGFLYPDHTHFFRVEPFVGANIEQAALDFTGIKLDHPLRMAVTEEHALTEIFR
                     GLRKSLKANSCKRAILVGHNSSFDLGFLNAAVNRTGIKRNPFHPFSSFDTATLAGLAY
                     GQTVLAKACQAAGIDFDGKEAHSARYDTEKTAELFCGIVNRWKEMGGWMDFDD"
BASE COUNT          621 a          956 c         1083 g          627 t
ORIGIN      
        1 cccgtggcgg cctgcgctgg tcggaccgtg aggaagactt ccgcaccgag gtgctgggcc
       61 tggtcaaggc gcagcaggtg aagaacgcgg tcatcgtgcc gatgggcgcc aagggcggct
      121 ttattccacg gcgtatgccg gccggcggca gccgtgatga tgtgctggcc gagggtattg
      181 cctgctaccg catctttatt tcgggcctgc tggatatcac cgacaacctg aaagacggcg
      241 ccgtggtgcc accgcaaaac gtggtgcgtc acgatggtga cgatccgtac ctagtggtag
      301 cggccgacaa aggcactgcg accttctccg atatcgctaa cggcattgct gctgaatatg
      361 gcttctggct ggacgatgcc ttcgcttcgg gcggctcggc tggttacgac cacaaaggca
      421 tgggcatcac cgccaagggc ggctgggtct cggtgcagcg tcacttccgt gagcgcggca
      481 tcgacgtgca gaaggacaac gtcactgtta tcggtatcgg cgacatggcc ggcgacgtat
      541 tcggtaacgg cctgttgctc tcggaaagcc tgcagctggt cgctgccttc aaccatatgc
      601 acatctttat cgaccctaac ccggacgcgg caaagagctt tgtcgagcgt cagcgcctgt
      661 tcgatctgcc gcgctccagc tgggccgatt acgacgccaa gctgatctcg gctggcggcg
      721 gcatcttcct gcgcagcgcc aagagcatcg ccatcagccc gcagatgaaa gagcgttttg
      781 acatcagcgc cgacaagctg gcgccgaatg agctgctgaa ctccttgctc aaggcgccgg
      841 tggatctgct gtggaacggc ggtatcggca cctacgtgaa atccagcaag gaaagccacg
      901 ccgatgtcgg cgacaaggcc aacgacctgt tgcgcgtgga tggccgcgaa ctgcgcgcca
      961 aggtggtggg tgagggcggt aacctcggta tgagccagct gggtcggatt gagtacggcc
     1021 tcaatggcgg caagagcaac accgacttta tcgacaacgc cggtggtgtg gactgctccg
     1081 accacgaggt aaacatcaag attctgctcg gcgaaatcgt cggcgctggc gacatgaccg
     1141 gcaagcagcg caacaagctg ctagtggaaa tgaccgatgc ggttggcggc ctggtgctgg
     1201 gcaacaacta aaagcagacc caggcgttgt ccctggccga gcgccgcgcc cgtgagcggg
     1261 tcggtgagta caaacgtttg atgaacgccc tggaagcggc cggcaagctg gaccgcgccc
     1321 tggagttcct gcccactgat gacgagctca acgagcgcgc cgccaacggc ctgggcctga
     1381 ctcgtccaga gctgtcggtg ctgatctcct acagcaagat cgacctcaag gaatcgctgc
     1441 tgaaatccct ggtgccggat gacgagtacc tggcgcgtga gatggaaacc gccttcccgc
     1501 cgctgctggc gaagaagttc ggcgagccga tgcgtcgcca tcgcctcaag cgcgaaatcg
     1561 tcagcacgca gatcgccaac gatctggtca accatatggg catcaccttc gtgcagcgct
     1621 tgaaggagtc caccggcatg agcgcggcga atgtcgccgg cgcctatgtg atcgtgcgtg
     1681 atctgttccg cctgccgcac tggtggcagc agatcgaggc gctggactac aaggtgccgg
     1741 ccgaactgca actgcagttg atggatgagc tgatgcgtct gggtcgccgc gctacccgct
     1801 ggttcctgcg cagccgccgt aatgatctgg atgccgctcg tgatgtcgcg catttcgcgc
     1861 cgcgtatcga agccctggtc ggccgccttg acgagctgtt ggaaggcccg gcccgtgaac
     1921 agtggctggc gcgcttccag gggtatgtcg aagccggtgt gccggaagag ctggcgcgtg
     1981 tagtggctgg caccagccat ctgtacaccc tgttgccgat catcgaagct tcggacgtga
     2041 ccgggcagga gccggatcgc gtagcagcgg cgtactttgc tattggcggg tcgctggagc
     2101 tgtcctggta tctgcagcag atcaccagcc tgccggtgga aaacaactgg caagcgctgg
     2161 cccgtgaagc cttccgcgat gacctggatt ggcagcaacg cgccatcacc gtgtcggtac
     2221 tgcaaatggc cgaaggcccg gcggatatcg aagagcgggt cggcgtgtgg ctggatcagc
     2281 accgccgcct ggtggatcgc tggaaggtca tgctcggcga gctgcgcgcg gccaccaaca
     2341 ccgattacgc catgtatgcc gtggctaacc gcgagctgat ggacctggcg caaagcgcct
     2401 gataggaaca gcgcaggcct ggcctgcgct gttcctgccc caataaaaag ccccgcaatc
     2461 gcggggcttt ttattggggc aggtctggtg tcggcgaaat tatgtttgtt ctatatctcg
     2521 cgagctagag tcctgcaagg caaaaataga cgaggaagcg gagtgtactt ttgtacatga
     2581 gcattccgag tctgttttta acgccgcagg gccgacgcgc agcagatcgt tgtcttgcac
     2641 ctacttagtc gtcgaagtcc atccagccgc ccatctcttt ccaacgattg acgatgccgc
     2701 agaacagctc ggccgtcttc tcggtgtcgt aacgggccga gtgcgcttcc ttgccgtcga
     2761 agtcgatacc ggcggcctgg caggccttgg ccagtacggt ctggccatag gcgaggccgg
     2821 ccagggtggc ggtgtcgaag ctggagaacg ggtggaacgg attgcgtttg atgccggtgc
     2881 ggttcaccgc agcattgaga aagcccaggt cgaagctgct gttgtggccg accaggatcg
     2941 cccgcttgca gctgttggct ttcagcgatt tgcgcagacc acggaagatt tccgtcagcg
     3001 cgtgctcttc agtgacggcc atgcgcagcg ggtgatcgag cttgatcccg gtgaagtcca
     3061 gcgccgcctg ttcgatattg gccccgacga agggctcgac ccggaagaaa tgggtgtggt
     3121 ccgggtagag aaaaccctgc tcgtccatgg cgatggtggt ggtggcgatt tccagcaggg
     3181 catcggtggc gcagttgaag ccgctggtct cgacatccac aaccaccggc aggtagccgc
     3241 gaaaacgtgc ggccatgggg tggcgcgggc cggaggggct gctttcc
//
LOCUS       DASRUM010000123         3555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1072564, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000123 DASRUM010000000
VERSION     DASRUM010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072564"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3555
                     /locus_tag="VFF22_08155"
     CDS             <1..>3555
                     /locus_tag="VFF22_08155"
                     /inference="COORDINATES: protein motif:HMM:NF017257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HZX16816.1"
                     /translation="AHGSLQAETEGTHTLLIEQGHFWQNQEKPKRATEVWNKLLLLDA
                     NQPDALYGLGLIAVQAAKPAEANQYLQRLQALQPLPRQALLLAQDIRLLDPANQQLLD
                     EARLLVESDERAKAVDVYRRALGGKPGQGQVGLEFYNNLGYVDQHWTEARRGMERLQR
                     EYPNDPYIALFLAKHLARNQGAREEGVRALAKLAEREDIGGDADESWRLALTWMGPPK
                     PAQQPLFEQFLRTHPDDEEIRALLIKGRTQPSAGSGAVAWRRDPLLDSGLKALEQDNL
                     AVAERDLTAYLKSHPNDADVLGGLGILRQRQERFEDAEVLLTKAVRQGGGRSWQRALD
                     DVRYWSLLQRARDNLARDKVADARQQLEQARRLNPNDSEALLALAELQAQQGELAAAE
                     AGYRQALAARSQEARALRGLAQVLGEQGKPDEALRLLERLPKAEREKLGGLGQLRAEQ
                     ALQRARAAEKRGDTSAMRQELETALRDDPNNAWARFALARLYVDLGAVTEARSLMDGL
                     LANKPDDRDALYTSALLSIQLNEWQQAQTTLARIPRNARNADINRLVADVEFNLQLQR
                     INELSRNGRKAEARAFLQRIEPQADAKPARLAAFAAAYADANDPQRAIAIMRDLLARS
                     PRADLSLTLSYASVLLEAEQDTEVAGILRDLQGRSMTVEQRQQYDDLLFYYRVRQAEQ
                     LRQRGELAAAYDTLAPALAQRPQDRLAVSALARMYGANGDTSKALELFKPLVKRHPDD
                     ANLLVGAADMAAQEAENAYAEDALERALKLAPNEVDILTTAARVYRFLGRTGTAAELL
                     GKVVAQEKLEQTPTFAANTRPAAAPANPFAGIGSATVAQEAIPAPVESLAVAPHKSAE
                     RPLELIPAPVEQQATRTAPALLAEPPTQQTPLRARADVQPASNPFVLNDDTAEVDPRA
                     GMSEAARALDDIMQQRSPYLVQGLTVRTNDSESGLSKITDIQAPFEASMPLGDNRLAV
                     RVTPVSLNAGSVGEGARDRFGGGPVATLANPGVAPGSQKDSGVGLAVAFENPGEGFKA
                     DLGTTPQGFLYSTAVGGVSLERSVAGSPNLHWSTKLSRRAVTDSLLSFAGAEDKRTGQ
                     RWGGVTANGGRAQLSFDDREVGAYAYVGMHRLMGHNVEDNDRFELGSGVYWYLQNDEQ
                     SQSTIGLNLLGIGYQENQGHFTFGHGGYFSPQTFFAIGV"
BASE COUNT          685 a         1022 c         1156 g          692 t
ORIGIN      
        1 ggcccacggc agtctccagg ccgagactga gggcacgcac accctgctga ttgagcaagg
       61 gcacttctgg caaaaccagg agaaacccaa gcgtgctacg gaagtgtgga acaagctctt
      121 gttgctcgac gccaaccagc ctgatgcact ctatggcctg ggcttgattg cggttcaagc
      181 ggccaaaccg gctgaggcca accagtattt acaacgtttg caggcgttgc agccgctgcc
      241 gcgtcaagcc ttgttgttgg cgcaggatat ccgcctgctg gaccctgcca atcagcagtt
      301 actggatgaa gcgcgcttgc tggtggagtc cgacgagcgt gccaaggcgg tggatgtata
      361 ccgtcgtgcg ctgggtggta aaccgggtca gggtcaggtt ggcctggagt tctacaacaa
      421 cctgggttac gtcgaccagc attggactga ggcgcgacgt ggcatggagc ggctgcagcg
      481 cgaatacccc aatgatccct atatcgcctt gtttttggcc aaacacctgg cgcgtaatca
      541 gggcgcgcgc gaggagggcg tacgtgccct ggccaagctg gctgagcgcg aggacattgg
      601 tggtgatgca gatgaaagct ggcgcctggc gctgacctgg atgggcccac ccaagcccgc
      661 gcagcaacca ctcttcgagc agtttctgcg cacccacccg gatgatgaag aaatccgtgc
      721 cttgttgatc aaagggcgaa cccagcccag tgctggtagc ggcgcagttg cctggcgccg
      781 tgacccgctg ctggacagtg ggctgaaagc cctagagcag gacaacctgg ccgtggctga
      841 acgtgacctg acggcctacc tgaaatctca cccgaatgat gccgatgtct tgggtggttt
      901 gggtattttg cgtcagcgtc aggaacgctt cgaagatgcc gaggtgctgc tgaccaaagc
      961 ggtcaggcag ggcggcggtc gttcctggca gcgtgcgttg gatgacgtgc gctactggtc
     1021 attgttgcaa cgtgcgcggg acaatctggc gcgtgacaaa gtcgccgatg cccggcagca
     1081 gctggagcag gcgcgccggc tcaatcctaa tgacagtgaa gctctcctgg cccttgctga
     1141 attgcaggcc cagcagggtg agttagccgc cgccgaagcc ggataccggc aggcattggc
     1201 agcgcgcagc caggaagccc gggcattgcg tgggctggcc caggttctgg gtgagcaggg
     1261 taagcccgat gaagcattgc gtttgctgga gcgcctgccc aaggctgagc gtgagaaact
     1321 cggtgggctt gggcagttgc gtgccgagca ggccttgcag cgcgctcgtg ctgccgaaaa
     1381 acgtggtgac acctccgcga tgcgtcagga gctcgaaacc gccctgcgcg atgacccaaa
     1441 caatgcctgg gcacggtttg ctctggcccg cttgtatgtc gacctgggtg ccgtcaccga
     1501 agcacgcagt ctgatggatg ggttgttggc caataaaccg gatgaccgcg atgcgcttta
     1561 caccagtgct ctgctgtcta tccagttgaa tgaatggcag caagcgcaaa ccacccttgc
     1621 ccgtattccg cgcaatgcac gcaatgccga catcaatcgt ctggtggcgg acgtggagtt
     1681 caacctgcag ctgcagcgta tcaatgaact cagccgcaat ggccgcaagg ccgaagcgcg
     1741 cgcgttcttg caacgtatcg agccgcaggc ggatgccaaa ccggcgcgtc tggccgcgtt
     1801 tgctgcggcc tatgccgatg ccaatgatcc gcagcgggcg attgccatca tgcgtgacct
     1861 gctggcccgc agcccgcggg cagatctgtc cctgacgttg agttatgcca gtgtgctgct
     1921 ggaagccgag caggatactg aggtcgccgg cattctgcgt gatttgcagg ggcgcagcat
     1981 gaccgtcgag cagcgtcagc agtatgacga tctacttttt tattaccggg ttcgccaggc
     2041 tgagcaattg cgtcagcgcg gcgaactggc ggctgcttat gacaccttgg cacctgcctt
     2101 ggcgcagcgc ccacaggatc gactggcagt gtcggcactg gcgcgcatgt atggcgccaa
     2161 cggtgatacg agcaaggcac ttgagttgtt caagccgctg gttaaacgtc atccagatga
     2221 tgccaacctg ctggtgggtg ccgctgatat ggcggctcag gaggctgaaa acgcttacgc
     2281 cgaagatgct ctggagcgtg cattgaagct ggcgcccaat gaggtcgata tcctcaccac
     2341 ggctgcccgt gtataccgtt tcctgggccg cactggaacg gctgccgagt tgctcggtaa
     2401 agtggtcgcc caggaaaagc tggagcagac tccgaccttt gcggccaaca ctcggccggc
     2461 cgcagcgccc gccaatccgt ttgccggcat tggctccgca accgttgcac aggaggcgat
     2521 tccagcgcct gttgagagct tggccgtggc gccgcacaaa agcgcggaac gtcctctgga
     2581 gttgatccct gctccggttg agcagcaggc cacgcgcaca gcccccgctt tgctggcgga
     2641 gccgccgact cagcaaacgc cattaagggc cagggcggac gtgcaaccgg ccagcaaccc
     2701 ttttgtcctg aacgacgata cggctgaggt ggacccgcgt gcgggcatga gcgaggccgc
     2761 aagggcgctg gacgacatca tgcaacagcg cagcccctat ctggttcaag gtctgacggt
     2821 acgtaccaat gacagtgaat cggggctgag caaaatcacg gatattcagg ctccctttga
     2881 agctagtatg ccgctaggtg ataaccgtct ggctgtgcgc gtcacccctg tttcgctgaa
     2941 tgccggcagt gtgggcgagg gtgcccgcga tcgctttggc ggtggcccgg tagcaacgtt
     3001 ggccaatcct ggcgttgctc ctggttcgca gaaagattct ggggttggcc tggccgtggc
     3061 gttcgagaac ccgggggagg gcttcaaggc tgacctgggc accacgccac aaggcttcct
     3121 ctatagcacg gcagtcggtg gcgtgagcct ggaacgcagt gttgccggta gcccgaacct
     3181 gcattggagc accaagctgt cgcgccgcgc cgtgacggac agcctgttgt cttttgccgg
     3241 tgccgaagat aaacgcaccg ggcagcggtg gggtggggta accgccaacg gtgggcgcgc
     3301 tcaactgagc tttgatgacc gtgaagttgg tgcctatgcc tatgtaggca tgcaccgctt
     3361 gatgggccac aacgttgagg acaatgaccg gtttgaactg ggctccgggg tgtactggta
     3421 cctgcagaat gacgaacaga gccagtcgac cattggcctc aacctgttgg gcattggcta
     3481 ccaggagaac caggggcact tcaccttcgg tcatggtggt tatttcagtc cgcagacgtt
     3541 ctttgccatc ggtgt
//
LOCUS       DASRUM010000124        44594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1892716, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000124 DASRUM010000000
VERSION     DASRUM010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 44594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44594
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1892716"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1967
                     /locus_tag="VFF22_08160"
     CDS             <1..1967
                     /locus_tag="VFF22_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HZX16817.1"
                     /translation="QPAIASRAGIEAEDLPLLRDWLREAGVRWGRDGTHREQLGLPAD
                     AAFTWRQGLDRLLLGFAAPPQLAGRQAPLLGASWPLDALEGGRAQLLGRLAGFVERLA
                     ALAQSLQRPRPLREWAESLQVLIDQLFDEREAGDTLLLLSKACAALKEQGDASGVERP
                     IELALIRQQLTAALEQGSAASGFLTGAVTFCTMVPMRSLPFRLVCLLGLDDGALPRRT
                     PAAGFDLIGQKPRRGDRARRLDDRYLLLETLLSARGGLYLSYVGRDPRSNAELPPSVL
                     VSELLDVVDLTAVCGVDGQNASTRITHHHPLQPFAPGNFAGNRHAGFAAPWFRAAQRL
                     SQAVEAPAPFASALDTPDAGGPGGAPLSIEPSQLIHCFKHPARYLLEQRLGLRLAQSE
                     ETLAGDEPFSVEWTSQHGLRQLALQAIEQGWSERQERAVAAASGWLPVGELGQAHWGQ
                     IRGPIRAFAPTLFDERPDDAPQPLLVDLELAGVRIHGWLDGVTSAGLFDYRLRDLGAW
                     ELAPFWLRHLLLNCAATPGINRDSRLLSPKSEWRLGPLADARGLLLPWLEAYKSALCE
                     PLPLFAKCSHGFARKWRKPGRKEPIDAARSEARLMWQGNDHMSGEGQDPWNALAFRDR
                     EPLDERFEALAEQLYGPALDALQGSDEDEA"
     gene            1964..5509
                     /gene="recB"
                     /locus_tag="VFF22_08165"
     CDS             1964..5509
                     /gene="recB"
                     /locus_tag="VFF22_08165"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HZX16818.1"
                     /translation="MSSASLNLLQDSFSGRSLIEASAGTGKTWTLTALYARLLLEKQL
                     SVSQILVVTFTTAATAELRERIRKRLAELLAVYDGVVSGDSLLNDLHAQYPDSASHRR
                     LLLAVHGFDEAAIFTIHGFCQRALQDAAFEAGGDFDNELTHDDREIIDALLADLWRHE
                     LAAAEPEWAAFLVQQKITPQSLRKRLRNHLGRPYLRIEPQPGASSEMSALCAAWQQAQ
                     RCWLSESAAWLAQLKAFDGFKSNMCNPAKLGVWQAELDGYFSDAAALFSKTEAHRRLA
                     REGLSKASKKGCEAPVNPLAAALQELCDALDAAQPEAEQRLIDLQVRLIGQLNEQLPA
                     RKAAQRLLAFDDLLNKLQQALQGEGGEHLAGTLRTQYPVALIDEFQDTDPVQYRVFHR
                     IYQDAGDLCFVGDPKQAIYAFRGADLATYLKARSEAARQYSLATNHRSTPELIAALNQ
                     LFDRPMPFAEPGLAYQQVGASERPRPQLVLPALEGEADAPLALVWLDNDYLGKGEASA
                     LVARDTARRIAAVLSASSRGEAYFEQDGTRTPLKGGDIAVLVASHRQAGEVAAELAAR
                     GVPSVRRGKENVWHSEEAAELSAVLAAYAEPGREGALRYALASRLLGRSAQVLAACAE
                     DAQAWDREREDAERYHQLWQQQGFMRAFRAWLDEQQVAQRLLALVDGERRLTNLLHLA
                     ELLQTESLQRPGLEQLLAWFNAQRSAEAHGEDALLRLESDAERVQIVTIHTSKGLEYP
                     LVFCPYLWDGALLRQHEDISCHADDGTPLLDLGSEQIDMHRERARHERFAEKLRLTYV
                     ALTRARDRLWLHWGPVDCKPKKDGTLGDSGLHSSALAWLLHGRGLSGEDALAELAGHL
                     QSLSPQGLRAEVEQLIASSQGQMVIQPLREGEASAAGEQRAPAPQQLATLQRSLHSAW
                     RIGSFSGLAAGMHMEAPDRDGLVLPDASEPGTGFFAFPRGARAGTCLHAILEDWARGK
                     GALNVLIEPGLSGHGINGDWQAVALDHLQQVIDADLDGNGLCLAGLNPARRLPELGFT
                     FPLAGLDVQRLRAILCDPAHGLAAPMREAAARLEFDSLKGFLKGFIDLTFEHDGRWYI
                     ADYKSNWLGPDASYYGGERLLQALAAEHYYLQYLIYLVALRRFLRQRLDDFQNDQLGG
                     AYYLFLRGMPTAGVYFSRPSDGLLDALDSLFAEGK"
     gene            5685..7520
                     /gene="recD"
                     /locus_tag="VFF22_08170"
     CDS             5685..7520
                     /gene="recD"
                     /locus_tag="VFF22_08170"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HZX16819.1"
                     /translation="MILSDLPLGPLERALVDSLQRLDPAASPVMLASAALLCMALDKG
                     DVCLPLARLAGQRPWPEHDFRLPPLGEWQAQLEASSLVGGDGDFTPLILEHGRLYLAR
                     YQAYERQLAEQLLLRAADLPEVDEAQLSESLTRLFAFNTQQPDWQRLAAAQAVRRKLA
                     VISGGPGTGKTTTVVRLLAALLEQPGCENLAIGLAAPTGKAAARMAEAIRNAKAQLPV
                     SESIKAALPDEARTLHRLLGSRGDSPQVRHHAANPLALDVLVVDEASMVDLALMAKLL
                     DALPPTARLILLGDKDQLCAVEAGAVFAELCEGRGFDAQAASELQRITGQQVPVSQPS
                     SQLGDAVVLLTHSHRFAGDSGIGELARRINGGDVSGTLNLLKENRSDLAWNAAPTPND
                     LLERLDQGYAPFISAAKSADPAAAFAAFNTFRALCAQREGSWGVAGINEALEARIKRR
                     SQVPSRERWYVGRPVMVRQNDYALGLFNGDIGICLHTEYGLRVFFEGEDGYRPFAPAR
                     LPSHDSAFAMTVHKSQGSEFSEVLLVLPEQPSPLLTRSLFYTGITRAKHKVEIWALPP
                     RLGEAVATRAERAAGLAERLQAKSAQPEVRAAAVKAPPANQLDLF"
     gene            7699..8457
                     /locus_tag="VFF22_08175"
     CDS             7699..8457
                     /locus_tag="VFF22_08175"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HZX16820.1"
                     /translation="MFARLTNILKGMLSLFIKDIERQNPEALLELEQENLRKQIANFN
                     QGLAAHAGLAERLMSQVRKQEAEERELHARTTAHLRAGNKSAAAQNALRLQNLARELE
                     ENRRQLAQAEETYQNLTKAREVAVAAARSKIEALKGAINDMRMKTAMAEMNEMAAGMV
                     SNIGGSGDTLNRLHEMVEEERTKAAGRARMARDSMDMSEVNIKEAEMDALADLALAEF
                     AAKEGISLPGQEATSEAPAASRTMGGSTPQSESQ"
     gene            8467..10116
                     /locus_tag="VFF22_08180"
     CDS             8467..10116
                     /locus_tag="VFF22_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX16821.1"
                     /translation="MSSEGKAPAWLDELRLAYESGAHGQFVLYGNVADRFPLDGRLVS
                     LTRYLDAKLFGTFDIVFLYDPGNGLSLLRGGERFAQWPAAEKIQPWPHDPREAVELIS
                     RYLRYRANLQALGRGDNEHVAVILRGAELVLPASRQGDFVTASLASLVRDWAAEPPFA
                     DMAFASLLIADNLNDLHPLVANNPRIDRVQVPLPDAASLGQCLAQLRIDHPVAFTAED
                     NGAVQALAGVSLSALASLVKRRAHEGKVLGAQDWIEAKKELVENDSAGLVEFIESKRT
                     LDDYHAQEALKVWLRQDMALWQASDLRALPKGYIFCGPVGTGKTYLVECLAGEAGVPV
                     VKLKNFRDRWVGSSEGNLEKIFRLIRGLGRCIVFIDEADQTLGKRDGGSGDSGLSGRL
                     YSMIAQEMGDADNRGKVIWILASSRPDLIEVDLKRPGRVDVKIPLLPTTTVEESAALL
                     KALLKRFELKLEIKALVKLPLPLLLTPGAAEALAVKVYRQVRTAQMEPLAALTHCLQG
                     YQPPVAEQVLRMQMSIAIREATDMAFVPESLRHLAQENLSL"
     gene            10113..10970
                     /locus_tag="VFF22_08185"
     CDS             10113..10970
                     /locus_tag="VFF22_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16822.1"
                     /translation="MSAGRYLWSAFNARPLGMPLPPNWFGLAAFALLGAFVSPGFWVL
                     GAGLELGYLLLLASSARFRRTVDARQVQPTDPASQRYEATVALLDSAQRARQLAVEAR
                     AREVLQLLSRSPLMASHAASLEQLVWLNLRLLVAGQALNVVVDTAAQDSAELQKQEDQ
                     IDARLAASELNDELRRSLEQQKQVIDARQAAHAEGLRRKEHVDAELQRIEQQIALIRE
                     QTLLATDEEQIGVALNALTSSFNEASRWLNSQRDLLGVLELNEQQALPRYVLQGQGDK
                     PAQAESLRQ"
     gene            11217..12899
                     /locus_tag="VFF22_08190"
     CDS             11217..12899
                     /locus_tag="VFF22_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HZX16823.1"
                     /translation="MYQSAPRQRQEGNAAGKLLTIVLVLGLLGLGGWLVSKDMLEEGA
                     GSVLSGLPASLGVAAGPVPVEPVSGTPTLQSAAPYEMKNNIIDIDMSEYAGYGGLIVA
                     NGGLAPNPESIFAKQYGFQVRLNKGESEEWSALNNGQMAAVATTADVLAVLGRQFEVT
                     VPAQIAFSRGADQVVVDTGIASINQLKGKVLGATQFNESEFFIRYLASEAGVPVKVLR
                     DLDARPAPNELGLVFYEDAFIACDAYAAELTGKQRLNGCVGWSPRTDEVVAESAGAAK
                     MLVSNRNLLVVADILVVNKGFATAHPEIVKGLVHGLLQGNRLLRDDPKTHAGVVAQAF
                     GWTPEETLDELSKVHLSNLPENLAFFDGSIDSAGSFQGIFQSSVLAYGSVIRNPADPA
                     RFADLQHLKALDQSGQFKGQSIAIAPIRTSGKVALEGDALLSKDIRFFFEANSAELDK
                     QAKDNQDYLDTIKQFLQVSPGSIVLLRGHVDNAMVADFERQGGSALVRNMALKAMELS
                     RQRTLAVKEALMARHPGIADDRIELVGRGWEEPLGQDSEQNRRVEVQWFTLE"
     gene            12959..13717
                     /locus_tag="VFF22_08195"
     CDS             12959..13717
                     /locus_tag="VFF22_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX16824.1"
                     /translation="MLQRLLVYLLSSLCLCAQAADLRLVTGDGYAPFTGQALPEGGML
                     TQVVRAALQRSSIDSSLDWQPWNRGHLKTLRGDYDATFPYVRTPQREEEFLYSEPLFI
                     AEQHIFSRAGDVIEIDDVPSMQGRRLCYPLGWQPPQIIQQLVDDGQLNRHSPAGLHEC
                     ARLLLMGRDDFFISDRRLGETALRLTGEPIERFRRSNSAINRSTLHLIVPRSHPQAAV
                     IIAQFNQGLAQLRASGDYQRMLERHQQPDESATP"
     gene            complement(13728..13994)
                     /locus_tag="VFF22_08200"
     CDS             complement(13728..13994)
                     /locus_tag="VFF22_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HZX16825.1"
                     /translation="MTETTAKRFIAGAVCPACSEMDKIQMWNVDGVPHRECVACGYAD
                     TLDERGNSVPRELPTRVNISALKPKAADPKVQVVQFFPNPKLKK"
     gene            complement(13991..16039)
                     /gene="prlC"
                     /locus_tag="VFF22_08205"
     CDS             complement(13991..16039)
                     /gene="prlC"
                     /locus_tag="VFF22_08205"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HZX16826.1"
                     /translation="MTANNPLLQAFDLPSYAAIRPEHVEPAVDSILADNRAAMSRLLE
                     QQSGTPSWDGLVLTLDELGARLGQAWSPVSHLNAVCNSPELRAAYEACLPKLSEYWTE
                     IGQNQPLFQAYQALAESPEAAGFDVAQKTILEHALRDFRLSGIDLPAEQQKRYGEIQM
                     KLSELASKFSNQLLDATQAWTKHITDEAALDGLTDSAKAQMKQAAEAKGLDGWLISLE
                     FPSYFAVMTYANDRALREEVYAAYCTRASDQGPNAGKNDNGPVMLEILALRQELAKLL
                     GFGNYAELSLASKMAETTDQVLHFLRDLGVRGKPFAEQDLRELQAFAAEQGCNDLQSW
                     DVGYYSEKLREQRYSISQEILRAYFPIDKVLSGLFAIVEKLYGIQIEELSGFDSWHPD
                     VRLFEIKENGAHVGRFFFDLYARANKRGGAWMDGARDKRRTSDGTLISPVANLVCNFT
                     PAVGGKPALLTHDEVTTLFHEFGHGLHHLLTRVEHAGASGINGVAWDAVELPSQFMEN
                     WCWEPEGLALISGHYETGEALPQDLLDKMLAAKNFQSGLMMVRQIEFSLFDFELHATH
                     GDGRSVLDVLEGIRQEVSVLRPPAYNRFPNSFAHIFAGGYAAGYYSYKWAEVLSADAF
                     SKFEEEGVFNSSTGRAFREAILARGGSQEPMVLFVDFRGRPPSIDALLRHLGLSQEAA
                     "
     gene            16212..16754
                     /locus_tag="VFF22_08210"
     CDS             16212..16754
                     /locus_tag="VFF22_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HZX16827.1"
                     /translation="MAIRTYQQFTPLLGPRAFVDASAVVLGDVEIGADSSVWPLVVIR
                     GDMHSIRIGERTSIQDGSVLHITHAGPFNPRGYPLNIGDDVTIAHNVTLHGCSIGSRV
                     LIGMGSIVMDGVVIDDEVMLGAGSLVPPGSHLESGFLYLGSPAKKARELSEKERSYFR
                     YSADNYVRLKDQHLMEGYAS"
     gene            complement(16751..18127)
                     /locus_tag="VFF22_08215"
     CDS             complement(16751..18127)
                     /locus_tag="VFF22_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZX16828.1"
                     /translation="MSYRLLSLPLLLALHGCTSQPPAVITPAAPAQWQHAPLASTQVA
                     PDWWQGFANPELNRLVDQALVANDDLAAAVARVRQAEASARMAGAPLLPQLNGNLGAG
                     REGRLGGDAEVAGNSYSASLAASYEVDLWGRLDAGRDSALADLRASQFDRAALQVTLS
                     AGVVSSWLQRVGLDQRLRIAGLNLDNAERVLRTVESRQRAGAATQLELAQQRGVVAEQ
                     RRALAALGQQTDDSRVALAVLLGQSSSASLNLAEQQLEALQLPSISAGLPSDLLLRRP
                     DLARAEAQLAAADADVQTARAALLPRLNLTATAGGNAGRVADVFADPLYSLGAALLAP
                     IFDGGYLAASRDLALAQREELLAAYRSSIVSAFADVQLALNGLAGIEAQWQAQQAVLA
                     QAELAFRLSESRYRAGADSLLTLLDTQRTLYAAQDDSAQLQLARLQAGVALYRALGGG
                     WQQVPVTR"
     gene            complement(18141..20108)
                     /locus_tag="VFF22_08220"
     CDS             complement(18141..20108)
                     /locus_tag="VFF22_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HZX16829.1"
                     /translation="MVSSTPLIELQDIRRCYGGHDGAPEVAVLRGVSLAIGAGEFVAI
                     VGASGSGKSTLMHLLGCLDRPTSGSYRFAGRDVAEFDADQLAWLRREAFGFVFQGYHL
                     IATESARENVEIPALYAGMSAEQRRERASALLSRLGLGERLDHRPSQLSGGQQQRVSI
                     ARALMNGGRIILADEPTGALDSHSGQEVMALLRELAAAGHTVILITHDRDVAAQAQRV
                     IEIRDGEIVSDSGAPDGRPQPSAQALPAPDMATAGYDQRLPWWKTFNEVARSAWRVMW
                     LNRFRTALTLLGIVIGVASVIVMLAIGLGTKQKVMAQMASMGATTLYLYAAQPPEGGP
                     WGVISEADMLAVAALPEVDLATPVIGNPRTVQYGNVSRDIYVYSSNEQLPGIHHWPLA
                     LGRFFNAAEDESLAPVVVLGHKARQQFFRDEPNPLGKQLLVGDAPFEVIGVMAEKGKE
                     SGADYYDEMVFIPYQAGRARVYQDILEPEYATLTAMSTSQVNAAGEAVRELLLARHGR
                     EDFQVDNAAAKVQAEADTQDSMTRMLGLIAAVSLLVGGIGVMNVMLMTVRERTREIGI
                     RMATGARQRDILQQFLGEAVLVTLVGGSIGVLLGLLVGAGLILWDVPLIFSLSAMLAA
                     FACAVATGLLFGYLPAKTAAALDPVVALAAE"
     gene            complement(20102..21271)
                     /locus_tag="VFF22_08225"
     CDS             complement(20102..21271)
                     /locus_tag="VFF22_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008093310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZX16830.1"
                     /translation="MNRLSRSILLRGLPALLVLLLLCGWLWRGESGPSFATEAVSRGD
                     VEATVVAIGTLQPRESVDVGAQVSGQVTRLHVEPADQVEKGQLLAEIDASLHLATVEA
                     DRAALDGLRAQLEEQQAQFDLAQQQHQRQQRLAVDEATREEDVQVAAASLKAAQARLK
                     QLRAQIAESQARLKGNEAQLSYTRLYAPIAGTVLGVDVKQGQTLNATYQTPTVLRIAD
                     LSAMTAWTKVSEADIRQLKPGLGLYFTTLGGDGRRWHAKIRQILPSPPLPPAASGDVG
                     GAPVAMKAVQYTVLFDVDNQDGELMPQMTAQVTFITAAARDVLTAPLEALQAVEGKSG
                     VFTARILNGKGRPEAREVRIGARSRQRVEVLDGLKENEQLITAELPDTRVARFQW"
     gene            complement(21300..21644)
                     /locus_tag="VFF22_08230"
     CDS             complement(21300..21644)
                     /locus_tag="VFF22_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3325 domain-containing protein"
                     /protein_id="HZX16831.1"
                     /translation="MDVLLASGLALSLCYAGLSGLCLAMNRHHQQVWARKAAARQHGL
                     RIAGWLLLALALWPCVLAWGAAVGVVLWLGWLALAGLLLVLLLPYRPRTAALLAALTL
                     LGSLPLLLRAAL"
     gene            complement(21644..23287)
                     /locus_tag="VFF22_08235"
     CDS             complement(21644..23287)
                     /locus_tag="VFF22_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HZX16832.1"
                     /translation="MTQALDQTVEKQGLRQAMAWLHTWGGLWACWVLFAIFLTGSLGV
                     FDDPITRWMKPHLPQASSLNGMDDGVDRPLAVRLGQAYLSEHQPQAHAWGIALPNDDD
                     KAVRLFWQDANEQFHNQRIDALSGQPLADANLRESEGGHHFVHMHFEFHGGTAGIWFV
                     GFFTMAMLVALVSGVIIHKRIFKDFFTFRPGKGQRSWLDAHNAVSVLSLPFQLMIAYT
                     GLATFYALYMPAGIAVHYPSDEAYFADLLTQPEPREETGVAATVTPLDMLLVRSEQQL
                     QRPLSFIRIEHPGDSSASVVTFGRFIEKSGEYRLLGDGSGSVHFDGVSGAQLDLQAPG
                     ELRGGAAQDVQSVMATLHFAGFGGYAARWLYFICGLAGAAMLASGAILFMVKRRQRSL
                     HEFGTRTPQVYRVIEVLNIGTIAGLSLACIAFFWANRLIPLGIAAREEWEIVAFFAVW
                     LATFGHAARRPANRAWTEQLLATALLCLALPLLNWLVTGEQFVSYLQQGDGERAGVEL
                     VAIAIGLLCLWTAQRVWRKSQVTQSRHRQTATASAVEVR"
     gene            complement(23284..23577)
                     /locus_tag="VFF22_08240"
     CDS             complement(23284..23577)
                     /locus_tag="VFF22_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16833.1"
                     /translation="MKPMPSSRRPALILLSRTAAAILGGYALAAAAVIFLGAILPLPK
                     GQAILAASLASFAVYTAAIVWVFAVQDNRRAWLGLLLPAVVLTLLGWWLGGGA"
     gene            complement(23776..26259)
                     /locus_tag="VFF22_08245"
     CDS             complement(23776..26259)
                     /locus_tag="VFF22_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HZX16834.1"
                     /translation="MFSCKSHTPAPRSQATATPQPLRQAIRYALLGAALASPVGAALT
                     APAAWAQTTAAEFDFAIPAGSLEAALMQFSTTAGVTVSFIPESAAGQQSAGLQGRYSA
                     GQALQALLAGSTLQAVEQANGSYSLLPRQSNGSLDLGATTVTAQSLGDTTEGSGSYTT
                     GSTSTATGLPLSIRETPQSISVITSQRMRDQGLTELTEVVRQTPGLTFNQAGSSGSDN
                     NAIYSRGFAVENYQIDGLQLLDSNYSNPAQSNDMVLYDRVEIIRGATGLTNGVGTPGA
                     TLNMIRKRPTFQPQSSITVKGGSWDYQRTEIDVSGPLTDSANVRGRVVAAYQDNESYI
                     DRLNERKKIFYGVIEADLTPDTLLTMGIDSQSNEVDDHARIGLPLFNQDGSRTDWDDS
                     DSAGATWAYSNRSAVSGFAILEHNLTERWKAKLSLNQRRSEYDELSGYAGGGNPQPDG
                     GGVILYANRWKAKPVQNSFDFNLAGSYDLFGREHDAIIGYSRQHTDYSTPAYTWHRPF
                     PEIDNIYSWDGSNPGRPPLPAYGRIDFSEEQTAAYGSTRLRISEDLSFILGARVIDWE
                     NREKIDNYDPATPDTDEQRSETGVVTPFAGVVYDLNDNWSLYGSYTSIFKPQSRKTLD
                     GAYIQPLEGVGYEVGSKAEFFDDRLNLGLALYQIEQDNLAVSIPGQFAPDGSQAYRAE
                     SGTKTRGFELEVSGELATDWQASASFSRNIVQDSEGQKLNTNIAQNTAKLFTTYTLRS
                     VGNGLTLGGGINWQSEIYSDGVGPSWDQRFTQDDYALVDVMARYPLTQQLTATLNLNN
                     LMNEEYYTSTSSSYYGTPRNATLGLRMEF"
     gene            complement(26370..27317)
                     /locus_tag="VFF22_08250"
     CDS             complement(26370..27317)
                     /locus_tag="VFF22_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HZX16835.1"
                     /translation="MAASLDLKVLEAAAQWYVDLRDAGLHTPLQQAHQQWLERDPLHR
                     QAWARVQKLEQTLGKVEPAVARPLLSGMRSNRRDTLKVLSVLLMASGSSALVWQAAPW
                     PQLLADYRTAPGERRSLRLDDGSQLTLDTHSAVDVRYSNTLREVQLHSGQILIETAKD
                     SLARPFIVHSAQGSIRALGTRFLVRSEGRRTYVDVLQHAVEVRPAELPEQTVRVASGQ
                     RLSFSAQHAGAPELAPPYADAWSRGLLVVSDWRLADFLAELSRYRPGHLRATPAVAGL
                     RISGAFQLTDIDGVLDNLATTLPVRVNRFTRYWAQLEAR"
     gene            complement(27323..27832)
                     /locus_tag="VFF22_08255"
     CDS             complement(27323..27832)
                     /locus_tag="VFF22_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003382231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZX16836.1"
                     /translation="MSTPDLAPQHRVHQLYHDHNGWLQGWLRKRLGCADKASDLAHDT
                     FVRLISQRDNPELREPRAYLSTIARSLMIDMFRRRSLEQAYLDTLASQPEQHDVSPET
                     RAIIVETLVEIDTLLDGLGGRAREIFLLAQLDGLSFVEIGRRLALSTNTVRKHFVRAM
                     THCLVLIED"
     gene            27967..28614
                     /locus_tag="VFF22_08260"
     CDS             27967..28614
                     /locus_tag="VFF22_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZX16837.1"
                     /translation="MHYQNILFDLDGTLTDPREGITRSVQHALAKLGIDEPDLQALEH
                     FIGPPLLQCFMQTYQFDEATAWQAVNHYRERFREVGLYENQLFDGVGELLQLLQSQQR
                     TLYIATSKPTVFAEQIARHFGFAQHFKVIYGSELDGTRTDKVELIAHLMEKEQLAPEA
                     TLMIGDRKHDLIGARRNGLDAAAVGYGFGSLAELQAEAPTYHFHSLAELHQAFVG"
     gene            complement(28633..28863)
                     /locus_tag="VFF22_08265"
     CDS             complement(28633..28863)
                     /locus_tag="VFF22_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012312060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HZX16838.1"
                     /translation="MNKFTLAVLLGLLSTSVLAAPKPCEELRAEIEVKIQAAGVASYT
                     LEIVPNEEAQDPNMVVGSCDGGTKKIIYQKNG"
     gene            complement(28928..29980)
                     /locus_tag="VFF22_08270"
     CDS             complement(28928..29980)
                     /locus_tag="VFF22_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HZX16839.1"
                     /translation="MFDALPWRWVPLAAALLLSGCSTLDYYSHLAGGHLQLLQAREPI
                     AELLENPAAAPELKQRLALAQQARDFASAQLSLPSNDSYRLYADIQRPFVVWNVFATP
                     EFSLSPELHCFPIAGCVAYRGFYSQGRARGSAALLKQQGLDIYLGGVEAYSTLGWFDD
                     PILNTMLRWSDERLVAVIFHELAHQQLYVADDTAFNESFASFVEREGLRQWRAKQGLQ
                     PSDPQGEQQREQFIALVMASRARLQQLYDSDLPPTQMRSGKQAEIARLRAEYHALSER
                     EWGGSGRYDDWINAPINNAKLLPFGLYDQWVPAFAALFEQVERDWPRFYERVAALGKL
                     PETERHQALAELSAGS"
     gene            30090..30509
                     /locus_tag="VFF22_08275"
     CDS             30090..30509
                     /locus_tag="VFF22_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16840.1"
                     /translation="MRYLAFLGAVLVLSACASPVPSPDPNQAWVELRSHAGTLLMADR
                     LDGQRLNDGRYFQVPAGAHELQARFQFEVNGGGGLDGTSEPRQVTCEIRVRYDNFLAG
                     QRYRLEARPMQMKAQAWLYDGQRNVLARGKILRCGTF"
     gene            complement(30522..30947)
                     /locus_tag="VFF22_08280"
     CDS             complement(30522..30947)
                     /locus_tag="VFF22_08280"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZX16841.1"
                     /translation="MKKTASAHWQGGIKDGKGTISTQSGALKSAPYGFNTRFEDQPGT
                     NPEELLGAAHAGCFSMALSKELGEAGMTAESIDTQAEVTLDKQDGGFAITAVHLTLKA
                     RIPGADRAAFEKAVETAKNGCPVSKVLNAEITLEAVLDS"
     gene            complement(31015..32010)
                     /locus_tag="VFF22_08285"
     CDS             complement(31015..32010)
                     /locus_tag="VFF22_08285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZX16842.1"
                     /translation="MSALAQSKFSTLDLAPVRDDGNAAQALHNSLALAQHVERLGFER
                     FWVAEHHNMDGIASSATAVLLGYLAAGTSTIRLGSGGVMLPNHAPLVIAEQFGTLATL
                     YPGRIELGLGRAPGADQFTAQALRRDRIGNADDFPADVEELQRYLGPRTPDQRVIAMP
                     GTDTNVPIWLLGSSLFSAQLAGEKGLPYAFASHFAPRYMHEAIRVYRNHFKPSAVLDK
                     PYVMLGVPLIAADTDEQAEYLATSAYQRILALIRGQSLVQKPPVASMQGLWLPHEKEA
                     VSSFLGMAMIGGPDKVRARLKVLLEQTGADELIFTCDLYDFADRLHAFELVAQLH"
     gene            complement(32167..32382)
                     /locus_tag="VFF22_08290"
     CDS             complement(32167..32382)
                     /locus_tag="VFF22_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HZX16843.1"
                     /translation="MSDHHTYKKIEIVGSSKTSIEEAINNALAEAAKSIRNLEWFEVT
                     ETRGHIVDGKVGHYQVTLKIGFRLSNS"
     gene            32543..33049
                     /locus_tag="VFF22_08295"
     CDS             32543..33049
                     /locus_tag="VFF22_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZX16844.1"
                     /translation="MRSPTTSKLGFFFSDHHRWLLQHIQRRLRNRADAEDTAAETFCQ
                     MLASSVDPDSIEQPRAYLSTIARRLIFDRHRRRQLELAYLERLASLPEALAPSPEEIA
                     LLVEALSAIDRTLAGLPQVVKKAFLYSQLDGFSYVEIGRRLGVSERTVGRYMTQALQQ
                     CYLTGMQP"
     gene            33046..34008
                     /locus_tag="VFF22_08300"
     CDS             33046..34008
                     /locus_tag="VFF22_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HZX16845.1"
                     /translation="MSQAVLTRAGEEAIGWMVRLRAATPDAQLQAGFDAWMVSDPAHA
                     AAWKRLQQGLGGHYETLRGFERRAPGQAREVLLRPDTSRREVLRGLVGLGLFGGGLWL
                     AGRSQPGQALLADLHTGTGERRAYSLADGSQLNLNAGSAVDLDFTARQRLLRLRHGEL
                     VVQVAPDPTRPFIVRSAQGDVRALGTRFLVRQEEQATRVLVLEHSVRLTLANGTTQEL
                     QEGQAALLHARHIALLGRAEQHRADWLQGQLSVLDEPLEAVIEALRPYRAGLIRVAPA
                     ARGLRVQGVYPLDNPERVLTALGETLPIRISHFGPWLTLIDAQH"
     gene            34142..36562
                     /locus_tag="VFF22_08305"
     CDS             34142..36562
                     /locus_tag="VFF22_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014591589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HZX16846.1"
                     /translation="MPKPWPRALAIAIALAAVGPFGYAHSEPATQQQTQRFDLPAASL
                     AETINAIARQSGQVVSLEPGLVRSKQAPAIRGELTATQALQQALSGSGLELRMTESGS
                     FSVQTKPQIDGALELDATTVTGQVVGATTEGSGSYAARAVTIGKGEHSLKEIPQSISV
                     ITRQQLDDQGITDLRDAVNQTTGLVGATGIGPGMVISSRGFQIDDWQYDGVPVPRNTY
                     VLGNWATQDLVFFDRVEILRGASGLLQGTGSPGGAINLVRKRGQNVPTASFTGKAGSW
                     DHYGLQADAGGPLNDSGSIRGRMVADQDDSHSFTDYVWSKTNSLYGALDFDLSLDTTL
                     GVGISNSDLDSRPMMRGLPRYPDGRDIGFSRSTYSGASWNRNDIEQTTVYADLEHRFD
                     DNWRFKTAALRMREHNDSLHQRMHGNVAADGSGLNFADWITNFESTKIGLDMFLNGKF
                     EALSLEQELTVGGNYAKYTSDDFYARRFVPGGNIFDIDHHRPEPTLNDILASPGGRAT
                     NAGYDVRQKGLYGSWRVKLAEPLTAVVGARVSWYDYAYMLPDENYKDSMSETGEVTPY
                     LGLVYDLSQEWAVYASYTDVFEPQNARTAQQQMLEPVIGSNYEVGLKGELFDGRVNTS
                     LAVFRYDQKNRAVTDEASGFACDGWYCSTASGKVRSQGIEAEISGEVLTDLQLFAGYT
                     YNTTKFIDDPENEGSIYSQWTPKHMLRMWADYRLPGDWSRVSVGGGFTSQSHTVSFDR
                     EFDVPGFTVWNARLGYRLSEEIDLAVNANNLFDKRYYLPGYAAADGSNNFGEPRNLML
                     SVKYTPAL"
     gene            complement(36583..37497)
                     /locus_tag="VFF22_08310"
     CDS             complement(36583..37497)
                     /locus_tag="VFF22_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX16847.1"
                     /translation="MSQDLPPLNALRAFEAAARLNSVSQAANELHVTHGAVSRQVRAL
                     EEHLGVALFSKEGRGLKLTDAGIRLRDISADLFSRLRSTCAELQQGQADAPFVLACPG
                     SLLARWFIPRLDRLNRELPELRLQLSASEGDLDPRRPGVDATLWFAEPPWPADMQVFE
                     LAAERIGPVLSPRYARFAELHLAPPAALLGEPLLHTSSRPQAWPSWAASNALDAAALK
                     LGQGFEHLYYLLEAAAAGLGVAIAPQQLVADDLAAGRLVAPWGFVETSARLALWVPIR
                     NRDNRAERLAQWLRKELDTPGQDVGGSR"
     gene            37632..38846
                     /gene="trpB"
                     /locus_tag="VFF22_08315"
     CDS             37632..38846
                     /gene="trpB"
                     /locus_tag="VFF22_08315"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HZX16848.1"
                     /translation="MTSFRTGPDANGLFGSFGGQYVAETLMPLIHDLAEEYEKAKLDP
                     EFQKELAYFQRDFVGRPSPLYFAERLTDMCGGAKIYFKREELNHTGAHKINNCIGQAL
                     LARRMGKKRIIAETGAGMHGVATATVAARFGMECVIFMGTTDIDRQQANVFRMKLLGA
                     TVIPVVAGTGTLKDAMNEALRDWVTNVHNTFYMIGTVAGPHPYPAMVRDFQAVIGKET
                     REQILAQEGRLPDSLVACIGGGSNAIGLFHPFLDDKDVQIVGVEAAGYGIETGKHAAS
                     LNGGVPGVLHGNRTFLLQNDDGQIIDAHSISAGLDYPGIGPEHAWLHDVGRVEYTSVT
                     DEEALAAFHTCCRQEGIIPALESAHALAEAFKRAPKLPKDHLMVINLSGRGDKDMQTV
                     MHHMENTSQEKH"
     gene            38846..39655
                     /gene="trpA"
                     /locus_tag="VFF22_08320"
     CDS             38846..39655
                     /gene="trpA"
                     /locus_tag="VFF22_08320"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZX16849.1"
                     /translation="MSRLQTRFAELKQQNRAALVTFVTAGDPNYDASLAILKGLPAAG
                     ADVIELGMPFTDPMADGPAIQLANIRALEGGQDLAMTLQMVREFREGEQATPLVLMGY
                     FNPIHKYGVERFIAEAVASGVDGLIVVDLPPEHNVDLCDPAQVAGLDFIRLTTPTTDD
                     KRLPKVLNGSSGFVYYVSVAGVTGAGSATLDHVEQAVARLRRHTDLPLCIGFGIRTPE
                     HAATIARLAEGVVVGSALIDQIANASSNEQAVDGVLSLCAALAEGVRGARG"
     gene            39882..40514
                     /locus_tag="VFF22_08325"
     CDS             39882..40514
                     /locus_tag="VFF22_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007967991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZX16850.1"
                     /translation="MSRPPIARKPRASSQSRIAGILAAARELLAEQGVASLSIYSVAE
                     RAQIPPSSVYHFFASVSALLEGLTADIHGAFRACLQEPIEHDQLRDWRDLSRLVEQRM
                     LAIYADDAAARQLILAQHGLAEVTHADRQHDIELGQLMQQLFDRHFPLPQLPEDVDVF
                     ALAMELGDRVYARSVQLHGEITPRMAEEGMRVFDAYLGLYLPPHLPKRNL"
     gene            complement(40597..41895)
                     /gene="argA"
                     /locus_tag="VFF22_08330"
     CDS             complement(40597..41895)
                     /gene="argA"
                     /locus_tag="VFF22_08330"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HZX16851.1"
                     /translation="MHDHVNWLRHASPYINAHRDCTFVVMLPGEGVAHPNFGNIVHDL
                     VLLHSLGVRLVLVHGSRPQIEARLASNGLTPRYHRDLRITDSPTLECVIDAVGQLRIA
                     IEARLSMDMARSPMQGSRLRITSGNFVTARPIGVVDGVDYHHTGEVRRIDRKGINRQL
                     DERSIVLLSPLGYSPTGEIFNLACEDVATRAAIDLDADKLLLFGAECGLLDEAGKLVR
                     ELRPQQVPAHLQRLGSSYQGELLDAAAEACKAGVRRSHIVSYAEDGALLSELFTRTGN
                     GTLVAQEQFESLREATIEDVGGLMELITPLEDQGILVRRSREVLEREIEQFSIVEREG
                     LIIACAALYQIADSDFGELACLAVNPAYRHGGRGDELLERIEERARAQGLKTLFVLTT
                     RTAHWFRERGFMPSSVDRLPAARASLYNYQRNSQVFEKAL"
     gene            complement(42004..43173)
                     /gene="argE"
                     /locus_tag="VFF22_08335"
     CDS             complement(42004..43173)
                     /gene="argE"
                     /locus_tag="VFF22_08335"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HZX16852.1"
                     /translation="MPLPSFKEQFAALIAAPSVSCTQAHWDQSNRAVIELLSSWLGDL
                     GFACDVQEISPGKFNLLASYGSGPGGLVLAGHSDTVPFDAALWQTDPLKLSEVDGRWV
                     GLGSCDMKGFFALIIEAVQPLLEQRFQQPLLILATCDEESSMSGARALAQAGRPLGRA
                     AVIGEPTGLRPIRLHKGIMMERIDILGQSGHSSDPNLGHSALEAMQDVMLELRGLRAQ
                     WQREYNNPQFSVPKPTLNFGCIHGGDNPNRICGQCSLEFDLRPLPGMQPEALRAAIRQ
                     KLQPLAEQHQVKIDFAPLFPSVPPFEQNADSELVRIAERLTGHTAAAVAFATEAPYLQ
                     QLGCETLVLGPGDIDCAHQPGEYLEMSRLQPTVRLLQQLIEHYCLQAKQPEPSGG"
     gene            complement(43241..44506)
                     /locus_tag="VFF22_08340"
     CDS             complement(43241..44506)
                     /locus_tag="VFF22_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HZX16853.1"
                     /translation="MSLIADYGLVLLLLACLFGFFMAWGVGANDVANAKGTSVGSKAL
                     TIKQAILIAMVFEFAGAYLAGGEVTETIKSGIVDASMITPDLMVLGMMSALLAAGTWL
                     LVASTKGWPVSTTHSIVGAVIGFAAVGVSMDAVHWSGVGPIVASWVVSPVLSGFVAFG
                     LFVSVQKLIIDTDHPFQNAKRFVPLYMFATGFMVSIMTLTKGLKHIGLDLSTTEGFFL
                     SLGVGGLIMLLGIILLSRIKIDVEADKDFHYASVEKVFAVLMIFTACAMAFAHGSNDV
                     ANAVGPLAAIVGVIQSGGEMAVGAKSAVPGWVLLLGAVGIVIGLATYGYKVIATIGKE
                     ITELTPSRGFAAELATATTVVGASAIGLPVSTTHTLVGAVLGVGLARGIGALNLGVIG
                     KIFMSWLITLPVGAALSIIFFYILRGIFL"
BASE COUNT         7918 a        14059 c        14565 g         8052 t
ORIGIN      
        1 aacagccggc catcgccagc cgtgccggta tcgaagccga agatctgccg ctgctgcgcg
       61 actggctgcg tgaagccggc gtgcgctggg gccgcgatgg cacgcatcgt gagcagcttg
      121 gcctgcccgc cgatgctgcg tttacttggc ggcaaggtct ggatcgcctg ctgctgggct
      181 ttgccgcgcc gccgcaattg gcgggcagac aggccccgtt gctgggtgcc agctggccgc
      241 tggatgccct ggaaggcggt cgtgcgcaac tgctcggccg tctagccggg tttgtcgaac
      301 gcctggccgc cctggcgcag agcctgcaac ggccacggcc tttgcgcgaa tgggccgaga
      361 gcctgcaagt gctgatcgac cagctgtttg atgagcgtga ggccggtgac accctgttac
      421 tgctgtccaa ggcctgtgcc gcgctcaagg agcagggcga cgcctcgggc gttgagcggc
      481 caatcgagct ggcgctgatc cgtcagcaac tcaccgccgc attggagcag ggtagcgccg
      541 cctccggttt tctcactggt gcggtgacct tctgcaccat ggtgccgatg cgcagcctgc
      601 cgtttcgtct ggtctgcctg ctcggcctgg atgacggcgc gctgccacgc cgcacccctg
      661 ccgccggctt cgacctgatc ggtcagaagc cccggcgcgg tgaccgcgca cggcgcctgg
      721 acgaccgcta tctgctgctg gaaaccctgc tctcggcaag gggcggcctg tacctcagct
      781 acgtcggccg cgacccgcgc agcaacgccg agctgccgcc ttcggtgctg gtcagcgaac
      841 tgctggatgt ggtcgacctc accgccgtct gcggtgtcga tggacaaaac gccagcacgc
      901 ggattaccca ccatcatcca ttgcaaccct tcgcccccgg caacttcgct ggcaaccgcc
      961 atgccggctt tgccgcgccc tggttccgcg ccgcgcagcg tttgagtcag gcggtggaag
     1021 cccccgcgcc ctttgccagc gccttagata cgccagatgc gggcgggccg ggcggcgctc
     1081 cgctaagcat cgaaccaagc cagttgattc actgtttcaa acacccggcg cgttacttgc
     1141 tggaacagcg cctcggcctg cgcctggcgc agagcgagga aaccctggcc ggcgacgagc
     1201 ccttcagcgt cgagtggacc agccaacacg gcctgcgtca gctggcgttg caggctatcg
     1261 agcagggctg gagcgagcgt caggagcgcg ccgtagctgc cgcgtctggc tggctgccgg
     1321 tgggcgaact gggccaggcc cactggggac agattcgcgg gccgatccgg gcctttgctc
     1381 caaccctgtt cgacgagcgc ccagacgatg ccccgcaacc gctgctggtg gatctcgaac
     1441 tggccggagt acgcatccat ggctggctcg atggcgtgac cagtgccggc ctgttcgact
     1501 atcgcctgcg cgacctgggt gcctgggagc tggcaccctt ctggttgcgt catctgctgc
     1561 ttaactgcgc cgccacgcct ggcatcaatc gcgacagccg tctgttgtcg ccgaaaagcg
     1621 aatggcgact cggtccgctg gccgatgccc gagggctatt gctgccctgg ctggaggcat
     1681 acaaaagcgc gctgtgcgag ccgctgccgc tgttcgccaa atgcagccat ggcttcgccc
     1741 gcaaatggcg caagcccggg cgcaaggaac cgattgacgc cgcccgcagc gaggcgcggc
     1801 tgatgtggca gggcaatgac catatgagcg gcgaaggtca ggatccctgg aatgccttgg
     1861 ccttccgcga ccgcgagccg ctggacgaac gtttcgaagc cctcgccgag cagctctacg
     1921 gcccggccct ggatgccctg caaggcagtg acgaggacga agcatgagca gcgccagcct
     1981 taacctgttg caggacagtt tcagcgggcg ctcgctgatc gaggccagtg ccggcaccgg
     2041 caagacctgg accctgacgg cgctgtacgc ccgtctgttg ctggagaagc agctcagcgt
     2101 cagccagatt ctggtggtga cctttaccac cgccgccacc gccgagctgc gcgaacgcat
     2161 tcgcaagcgt ctggccgagc tgctggcggt gtatgacgga gttgtcagcg gcgacagcct
     2221 gcttaacgat ctgcatgccc agtatccgga ctcggccagc catcggcgtt tgctcctggc
     2281 ggtgcatggc ttcgacgagg cggcgatctt caccatccac ggcttctgcc agcgtgcgct
     2341 gcaggatgct gcgttcgagg ccggtggcga tttcgataac gagctgaccc atgacgaccg
     2401 cgagattatc gacgccctgc tcgccgatct ctggcgccat gaactggccg ccgccgagcc
     2461 ggaatgggcg gcgtttctgg tgcagcagaa aatcaccccg cagagcctgc gcaaacgttt
     2521 gcgcaatcac ctgggcaggc cgtatctgcg tatcgagcca caacctggcg ccagcagcga
     2581 gatgagcgcc ctgtgcgccg cctggcagca ggcgcagcgc tgctggctga gtgaaagcgc
     2641 cgcttggctg gcgcagctca aagccttcga tggctttaaa agcaatatgt gcaacccggc
     2701 caagctgggc gtttggcagg ctgagctgga tggttatttc agcgatgcgg cagcactgtt
     2761 tagcaaaacc gaagcgcacc gccgcctagc ccgcgaaggg ctgagcaagg ccagcaaaaa
     2821 gggctgcgaa gccccggtca atcccctggc tgctgcgttg caggaactgt gcgatgcgct
     2881 ggatgccgcg cagcccgagg ccgagcaacg gctaatcgac ctgcaggtac gcttgatcgg
     2941 ccagctcaac gagcaactgc cggcgcgcaa ggccgcgcag cgtctgctgg ccttcgatga
     3001 cctgcttaac aaactgcagc aggcgctgca gggagagggt ggtgaacacc tggccggcac
     3061 gctgcgtacg caataccccg tggcgctgat cgacgagttt caggacaccg acccggtgca
     3121 gtaccgggtg tttcaccgca tctatcagga tgccggcgac ctgtgttttg tcggtgaccc
     3181 caagcaggct atctacgcct tccgtggcgc ggatctggcg acttacctta aggcgcgcag
     3241 cgaagccgcg cggcaataca gcctggccac caaccatcgc tccacacctg agctgatcgc
     3301 cgcgcttaac cagctattcg accgccccat gccttttgcc gaaccgggcc tggcctatca
     3361 gcaggtcggt gccagcgagc ggccgcgtcc gcagttggta ttgccggcgc tggaaggcga
     3421 ggcagacgcg ccgttggcgc tggtctggct ggataacgac tatctgggta agggtgaagc
     3481 gagcgctttg gtggcgcgag acaccgcgcg gcgtatcgct gcggtgctaa gcgccagcag
     3541 ccgtggcgag gcgtattttg agcaagacgg cacgcgcacc ccgcttaaag gcggggatat
     3601 cgccgtgctt gtggccagcc accgtcaggc tggcgaagtg gccgctgagt tggccgcacg
     3661 tggcgtgccc agcgtacgcc ggggtaaaga gaacgtctgg cacagcgaag aggccgctga
     3721 gctgtccgcc gtgctcgctg cttatgccga accgggccgc gaaggtgcgt tgcgctatgc
     3781 cctggccagc cgcctgctgg gccgcagcgc tcaggtgctg gctgcttgcg ccgaggacgc
     3841 ccaggcttgg gatcgcgagc gcgaagatgc cgagcgttac catcagctct ggcagcaaca
     3901 aggctttatg cgcgccttcc gcgcctggct ggatgagcag caggttgccc agcgcctgct
     3961 ggccctggtg gacggcgagc gccgcctgac caacctgctg cacctggccg agctgttgca
     4021 aactgaaagc ctgcaacgcc ccggtctgga acagttgctc gcctggttta acgcccagcg
     4081 cagcgccgag gcccatggcg aagacgcctt gctgcgtttg gaaagcgatg ccgagagggt
     4141 gcagatcgtc accatccaca cctccaaggg cctggaatac ccgttggtgt tctgccccta
     4201 cctgtgggac ggcgcgctgc tgcgtcagca tgaggacatc agctgccacg ccgacgacgg
     4261 cacaccgctg ctggacttgg gcagcgagca gatcgacatg caccgcgaac gcgcccgtca
     4321 tgagcgtttc gccgaaaaac tgcgcctgac ctatgtggcc ctgacccgcg cccgggatcg
     4381 cctgtggctg cactgggggc cggtggactg caagccgaaa aaggacggca ccctcggcga
     4441 cagcggcctg cacagcagcg ccctggcctg gctgctgcac ggccgtgggt tatcgggtga
     4501 ggatgcgttg gccgagctgg ccggccatct gcaaagcctg agtccgcaag ggctgcgtgc
     4561 agaagtcgaa caactgatcg ccagtagtca gggccagatg gttattcagc cgctgcgtga
     4621 gggcgaggcc agcgccgccg gtgagcagcg cgcgccagcc ccgcagcaac tcgccaccct
     4681 gcagcgcagc ctgcacagcg cctggcgtat cggcagtttc tccgggttgg ccgccggcat
     4741 gcatatggaa gcgccggatc gcgacggtct ggtgctgccg gatgccagcg agccgggcac
     4801 gggctttttc gccttccccc gtggcgcgcg ggccggcacc tgcctgcatg ccatcctcga
     4861 agactgggcg cggggcaaag gcgcgctgaa tgtgctgatc gaaccggggc tgagcggcca
     4921 cggtatcaac ggcgactggc aggccgtggc gcttgaccat ctgcaacagg tgatcgatgc
     4981 cgatctggat ggcaatggcc tgtgccttgc cggccttaat ccggcgcggc gtttgcccga
     5041 gctgggtttt acctttcccc tggccgggct ggatgtgcag cgcctgcggg cgattctctg
     5101 cgatccggcc catggcctgg ccgcacccat gcgtgaggcg gcggcgcgcc tggaattcga
     5161 cagcctcaag ggctttctca agggctttat cgacctgacc ttcgagcacg acgggcgctg
     5221 gtatatcgcc gactacaagt ccaactggct cggcccggac gccagctact acggcggcga
     5281 acgcctactg caggccctgg ccgccgagca ttactacctg caatacctga tctatctggt
     5341 ggcgctgcga cgcttcctgc gtcagcgtct ggacgacttc cagaatgacc agctgggcgg
     5401 tgcctactac ttgtttctgc gcggcatgcc gacggctggg gtgtattttt cgcggcccag
     5461 cgatgggctg ctggatgcgc tggatagtct gtttgcggag ggcaaatgat gctgcttcgt
     5521 aacccgtatg caatccggga gtggccttca cgccgcccga cccattcccc ggatttcatc
     5581 cgggctacgg gctggctatc cgtagggtgc gccgtgcgca ccatggttat tgcaattggt
     5641 gcgcacaacc taggcggccc cgcggctccc tacggagaga ttgcatgatc ttatccgacc
     5701 tccccttagg cccgctggag cgcgccctgg tcgacagcct gcaacgcctc gatccggcgg
     5761 cctcacctgt gatgctggct tcggcggcgc tgctttgtat ggcgctggac aagggcgacg
     5821 tgtgcctacc cctggcgcgc ctggccgggc agcgcccctg gcccgagcat gatttccgcc
     5881 taccgccttt gggcgaatgg caggcgcagc ttgaagcctc gtccctggtg ggcggcgacg
     5941 gtgatttcac cccgctgatt ctcgagcatg gccgcctcta tctggcgcgc taccaggcct
     6001 acgaacgcca gttggccgaa cagttgttgc tgcgtgctgc cgatctgccc gaggtcgatg
     6061 aggcgcaact gagcgagagc ctgacccggc tgtttgcttt caatacgcaa cagcccgatt
     6121 ggcagcgttt ggccgccgcg caagcggtgc ggcgcaagct agcggtgatc tccggcggcc
     6181 ccggcaccgg taaaaccacc accgtggtgc gcctgttggc tgcattactt gagcaacctg
     6241 gctgcgaaaa tctagccatc ggcctggcag cgcctacggg caaagctgct gcgcgtatgg
     6301 ccgaggcgat ccgcaatgcc aaggctcagc tgccggtcag cgaatcgatc aaggcggcgc
     6361 tgccggatga agcgcgcacc ctgcaccgcc tgctcggcag ccgtggcgac agcccgcagg
     6421 tgcggcatca cgcggccaac ccgctggcgc tggatgtgct ggtggtggac gaagcctcga
     6481 tggtcgacct ggcgctgatg gccaaactgc tcgatgcgct gccgcccact gcccggctga
     6541 ttctgctcgg tgacaaagac caactgtgcg ccgtggaagc cggcgcggta ttcgccgagc
     6601 tctgcgaggg ccgtggtttc gatgcccagg ctgctagtga gctgcaacgt atcaccggcc
     6661 agcaagtgcc ggtcagtcag ccaagctcgc aactcggcga tgccgtggtg ctgctcaccc
     6721 acagccaccg ttttgccggc gatagcggca tcggcgagct ggcgcggcgg atcaacggcg
     6781 gcgacgtcag cggcaccctg aatctgctta aggaaaatcg cagcgacttg gcctggaatg
     6841 ctgcgccaac gccaaacgac ctgctggagc gtctggatca aggctacgca ccctttatca
     6901 gcgccgcaaa aagcgccgac cctgcggcag cctttgccgc gttcaacacc ttccgcgcac
     6961 tctgcgccca gcgcgaaggc agctggggcg tggccggtat taacgaagct ctggaagcac
     7021 ggatcaaacg ccgcagtcag gtgcccagcc gcgaacgctg gtacgttggc cggccggtga
     7081 tggtgcggca gaacgattac gccctcggcc tgtttaacgg cgatattggc atttgcctac
     7141 acactgagta tggcctgcgg gtgttctttg aaggcgagga cggctaccgt cccttcgccc
     7201 cggcacgcct gcccagccac gacagcgcct tcgccatgac cgtgcacaaa agccagggct
     7261 cggaattcag tgaagtgctg ctggtgctgc ctgaacaacc cagtcccctg ctgacccgca
     7321 gcctgttcta caccggcatc acccgcgcca aacacaaggt cgaaatctgg gccctgccgc
     7381 cgcgcctggg cgaggcggtg gcgacccgcg ccgagcgggc ggcgggcttg gctgagcggc
     7441 tgcaggcaaa atcggcgcag ccggaagtgc gagccgcggc ggttaaagcc cctccggcca
     7501 atcaactcga tctattctga tggctgcaga cgtgaccgac tggctatccc gaagttgtcg
     7561 cctctgccta caatcgccag cagcactaac cggcgacgga cggttgcatc aattgccagg
     7621 ctcaccaaga atgccccacg cttgaccggc cgcgccgtca gtcatcgatg gattgcccgc
     7681 ttactgcaag gagacaccat gttcgctcga ctgaccaata tcctcaaagg catgctgtcg
     7741 ctgtttatca aggacatcga gcggcagaat ccggaggcgt tgctggagct ggagcaggaa
     7801 aacctgcgca agcagatcgc caatttcaac cagggcctag ccgcccacgc gggcctggcc
     7861 gagcgcttga tgagtcaggt gcgcaagcag gaagccgagg agcgtgagct gcatgcgcgc
     7921 accactgccc acctgcgcgc cggcaacaag tcggccgcgg cgcagaacgc cctgcgtctg
     7981 cagaacctgg cccgcgagct ggaagagaat cgccgccagc tggctcaggc cgaagagact
     8041 taccagaacc tgaccaaggc ccgtgaagtg gcggtggccg ccgcacgcag caagattgaa
     8101 gcgctcaaag gcgcgatcaa cgatatgcgc atgaagaccg cgatggccga gatgaacgag
     8161 atggccgctg gcatggtgag caatatcggt ggttccggcg ataccctcaa ccgcctgcac
     8221 gagatggtcg aggaggaacg caccaaggcc gctggccgcg cgcgcatggc tcgcgattcg
     8281 atggacatga gcgaagtgaa tatcaaggaa gccgagatgg acgccctggc cgatctggcc
     8341 ctggccgagt tcgctgccaa ggaaggcatc agcctgccgg gccaggaggc caccagcgag
     8401 gcgccggcag ccagccgtac gatgggcggc agcactccgc aatccgaaag ccagtaaggc
     8461 gcgcccatga gcagtgaagg caaagcaccc gcctggctcg acgaactacg cctggcctat
     8521 gagagcggcg cccacggcca gttcgtgctg tatggcaatg tcgccgaccg cttcccgttg
     8581 gatggtcgcc tggtcagcct gacccgttac ctcgacgcca agctgttcgg tactttcgat
     8641 atcgtttttc tctacgaccc cggcaatggt ctcagtctgt tgcgcggcgg cgagcgtttt
     8701 gcccagtggc cggcggcaga aaagatccag ccctggcccc atgacccgcg cgaggcggtt
     8761 gagctgatca gccgctacct gcgctaccgc gccaatctgc aagccctcgg tcgtggcgac
     8821 aacgaacatg ttgcggtgat tctgcgcggt gctgagctgg tgctgccggc cagccgccag
     8881 ggcgacttcg ttaccgccag cctggccagc ctagtgcgcg attgggccgc cgagccgccg
     8941 ttcgccgata tggccttcgc cagcctgctg atcgccgaca acctcaacga cctgcatccg
     9001 ctggtggcca ataatccgcg catcgaccgg gtgcaggtgc cgctgccgga tgctgcgagc
     9061 ctcggccagt gcctggcgca actgcgcatt gatcatcccg tcgccttcac cgccgaggac
     9121 aacggcgccg tgcaggccct cgctggagtc agccttagcg ccctggccag cctggtcaaa
     9181 cgccgcgccc atgaaggcaa ggtgctcgga gcccaggatt ggatcgaagc caagaaggaa
     9241 ctggtggaaa acgacagtgc cggcctggtg gaatttatcg aatccaagcg caccctcgac
     9301 gactaccatg cccaggaggc gctgaaagtc tggctgcgtc aggacatggc gttgtggcag
     9361 gccagtgatc tgcgcgcgct gcccaagggc tatatctttt gcggcccggt gggcaccggc
     9421 aagacctacc tggtcgagtg cctggccggc gaggctgggg tgccggtggt caagctgaag
     9481 aacttccgcg accgctgggt cggctccagc gaaggcaatc tggagaagat tttccgcctg
     9541 atccgtggcc tcggtcgctg catcgtgttt atcgacgaag ccgaccagac tctcggcaag
     9601 cgcgatggcg gcagcggcga cagcggcttg tccgggcgcc tctactcgat gatcgcccag
     9661 gaaatgggcg acgccgacaa ccgcggcaag gtgatctgga ttctcgcctc ctcgcgcccg
     9721 gatctgatcg aagtcgacct caagcgccct ggccgagtcg atgtgaaaat tccgctgctg
     9781 ccgaccacca cggtcgagga aagcgcagcg ctgctcaagg ccctgctcaa acgcttcgag
     9841 ctcaaactgg agatcaaggc gctggtgaaa ctgccgctgc ctttgctgct taccccaggc
     9901 gccgccgagg ccctggcggt taaggtctac cgccaggtgc gcaccgcgca gatggaaccc
     9961 ttggcggcgc tcacgcattg cctgcagggt tatcagccgc cggtggccga gcaggtgttg
    10021 cgcatgcaga tgagcatcgc catccgcgaa gccaccgata tggccttcgt gcccgagtcg
    10081 ctgcgccacc tcgctcagga aaatctcagc ctatgagtgc cggacgctac ctctggtcgg
    10141 ccttcaatgc ccgcccgctg ggcatgccgc tgccgccgaa ctggtttggc ctggcggcgt
    10201 tcgctttgct tggcgccttt gtcagccccg gcttctgggt gctcggcgcg ggcctggagc
    10261 tgggttatct gctgctgctg gcgagcagcg cgcgttttcg ccgaaccgtg gatgcccggc
    10321 aagtgcagcc gaccgatccc gccagtcagc gttatgaagc gacagtggca ctgctcgata
    10381 gcgcgcaacg cgctcgtcag ctggcggtcg aggcacgtgc ccgcgaggtg ttgcaactgc
    10441 tctcgcgttc gccgctgatg gcctctcatg ccgctagcct ggaacaactg gtgtggctca
    10501 atctgcgtct gctggtcgcc ggccaggcac tgaatgtggt ggtggacact gccgcccagg
    10561 acagcgccga actgcagaag caggaagacc agatcgacgc gcgtctggcc gccagcgaac
    10621 tgaacgacga gctgcggcgc agcctggaac agcaaaagca ggtgatagac gcgcgccagg
    10681 cagcccatgc cgaaggcctg cggcgcaagg agcatgtgga tgccgagttg cagcgcatcg
    10741 agcagcagat cgccttgatc cgcgaacaga ctctgctcgc caccgacgaa gagcagatcg
    10801 gcgtggcgct caacgccctg acttcctcgt tcaacgaggc cagtcgctgg ctgaatagtc
    10861 agcgcgacct gcttggcgtg ctcgaactca acgaacaaca ggccctgccg cgttatgtat
    10921 tgcagggcca gggcgacaaa ccggcgcagg ccgaatcgct gcgccagtaa cggggcagtg
    10981 agattagatg tagcccggat aagccttggc gcaatccggg atcgcggcac cccggattgc
    11041 gctgcgctta tccgggctac ggggctgatg gcacagacgg cggacaagcc ttcggcatgt
    11101 ccgccctacg gatttgcgta agtcttgtag atccggatgc aatccgggaa gcggttcagg
    11161 ctgtgaacac ttttgattgg cgggccgctg gctcgcatcc gacagggaga ggcgtcatgt
    11221 atcaatccgc acccaggcag cgccaagagg gcaatgccgc cggcaagttg ttgaccatag
    11281 tgctggtgct cggcctcctt ggcctaggcg gctggttggt cagcaaggac atgctcgaag
    11341 agggcgccgg tagcgtgctc agcggtttgc ccgcgagcct cggagtggcg gcagggcctg
    11401 tgccggttga gccggtaagc ggcacgccga ccctgcaatc cgccgcaccc tatgagatga
    11461 agaacaacat catcgacatc gacatgagcg agtacgccgg ttacggcggc ctgatcgtcg
    11521 ccaatggcgg gctggcgccc aaccccgagt cgatcttcgc caagcagtac ggcttccagg
    11581 tgcgcctgaa caagggcgag agcgaggaat ggtcggcgct gaacaacggc cagatggccg
    11641 cagtcgccac taccgccgat gtgctggcgg tgcttggtag gcagttcgag gtcacggtgc
    11701 cggcgcagat cgccttctcc cgtggcgccg accaggtggt ggtggacacg ggcatcgcca
    11761 gcatcaacca gctcaagggc aaggtgctcg gtgctaccca gttcaacgag agtgagttct
    11821 ttatccgcta cctggcctcc gaagccgggg tgccggtcaa ggtgctgcgc gacctggacg
    11881 cccgcccggc gccgaacgag ctgggcctgg tgttctacga ggatgccttt atcgcctgcg
    11941 atgcctacgc cgccgaactg accggcaagc agcgcctgaa cggctgcgtc ggctggtcgc
    12001 cgcgcaccga tgaagtggtc gccgaatccg ctggtgccgc gaagatgctg gtgtccaacc
    12061 gcaacctgct ggtggtcgcc gatattctgg tggtcaacaa gggctttgct actgcccacc
    12121 cggaaatcgt caaaggcctg gtgcatggcc tgctgcaagg caaccgcctg ctacgcgacg
    12181 atcccaagac ccatgccggc gtggttgctc aggcgttcgg ctggacgcct gaggaaactc
    12241 tcgacgagct gagcaaggtg cacctgagca acctgccgga gaacctggcg ttcttcgacg
    12301 gcagcatcga ttcggccggc tcgttccagg gcatcttcca atcttcggtg ctggcttacg
    12361 gttcggtgat ccgcaacccg gccgacccag cacgcttcgc cgacttgcag cacctcaagg
    12421 cgctggatca gagcggccag ttcaaaggcc agagcatcgc catcgcgccg atccgcacca
    12481 gcggcaaggt ggctttggaa ggcgacgcgc tgttgagcaa ggacatccgc ttcttcttcg
    12541 aggcgaactc ggctgagctg gacaagcagg ctaaggacaa ccaggactac ctggacacca
    12601 tcaagcaatt cctccaggtc agccctggct ccatcgtgct gctgcgcggc catgtggata
    12661 acgccatggt cgccgacttc gagcgtcagg gtggctcggc actggtgcgc aacatggcgt
    12721 tgaaggccat ggagctgtcg cgccagcgca ccttggcggt caaggaagca ttaatggcgc
    12781 gccaccctgg catcgctgac gatcgcatcg agttggtcgg ccgcggctgg gaagagcctc
    12841 tgggtcagga cagtgagcag aaccgtcgcg tcgaggtgca gtggtttacc ctcgaataaa
    12901 ggcgccgctg tgcacggcgc gccctagact aaatccatag ctcctggata tcggcccgat
    12961 gctgcagcgc ttgctggtct acctgttgtc gagcctgtgc ctctgtgctc aggccgccga
    13021 cctgcgcctg gttaccggcg atggctatgc gccctttacc ggtcaggcct tgccggaggg
    13081 tggcatgctc actcaggtgg tgcgggcggc gttgcagcgc agcagtatcg acagcagcct
    13141 ggattggcag ccatggaacc gcggccacct gaaaaccctg cgcggcgatt acgacgcgac
    13201 tttcccttat gtacgcaccc cacagcgtga agaggagttt ctctactcgg agccgctgtt
    13261 tatcgccgag caacatattt tcagccgcgc gggtgatgtg atcgagatcg acgatgtgcc
    13321 tagcatgcag ggccgccgcc tgtgttaccc gctgggttgg cagccgccac agatcattca
    13381 gcaactggtc gatgatgggc aactaaaccg gcattccccg gcggggctgc acgagtgtgc
    13441 gcgtctgcta ttgatggggc gcgatgattt ctttatctcc gaccggcgcc tcggcgaaac
    13501 cgcgttgcgc ttgaccggtg agcccatcga acgctttcgc cgctccaaca gcgcgatcaa
    13561 ccgcagcacc ctgcacctga tcgtgccacg cagccacccg caggcggcgg tgatcatcgc
    13621 gcagttcaac cagggcttgg cgcaattgcg cgccagtggc gattaccagc gcatgcttga
    13681 gcgccatcag cagccggatg agagcgctac gccctgagct ggcgcagcta ttttttcagc
    13741 ttggggttgg ggaaaaactg cacgacttgc accttgggat ccgccgcctt gggttttagc
    13801 gcgctgatat tgacccgcgt gggcagttcg cggggtaccg agttgccgcg ctcgtccagg
    13861 gtgtcggcat agccgcaggc cacgcattcg cggtgtggta cgccatcgac attccacatc
    13921 tggatcttgt ccatttcgct gcacgccggg cagaccgccc cggcgataaa gcgttttgcc
    13981 gtggtctctg tcatgcggcc tcctggctca agccaaggtg gcgcagcagg gcgtcgatgg
    14041 atggcgggcg gccacggaag tcgacgaaca gcaccatcgg ttcttgcgag ccgccacgag
    14101 caaggatcgc ctcacggaag gcgcgaccgg tggaactgtt gaacacccct tcttcctcga
    14161 atttggagaa ggcgtcggcg ctgagcactt cggcccactt gtagctgtaa taaccggccg
    14221 cgtaaccgcc ggcgaagata tgcgcgaagc tgttggggaa gcggttgtag gccggcggcc
    14281 gtagcaccga gacttcctgg cggatgcctt cgagtacgtc cagcacgctg cgaccgtcgc
    14341 cgtgggtggc gtgcagttcg aagtcgaaca ggctgaattc gatctgccgc accatcatca
    14401 gcccggactg gaagttcttc gctgccagca tcttgtccag caggtcctgc ggcagcgcct
    14461 cgccagtctc gtagtgaccg gagatcagcg ccaggccctc gggctcccag caccagttct
    14521 ccataaactg gctcggcagc tcgaccgcat cccaggccac gccgttgata ccggacgcgc
    14581 cggcatgttc gacgcgggtc agcagatgat gcaggccatg gccgaattcg tggaacaggg
    14641 tggtgacttc atcgtgggtc agcagcgccg gcttgccgcc gactgccggg gtgaagttgc
    14701 acaccaggtt ggccaccggg ctgatcagcg tgccatcgga cgtgcggcgc ttgtcacgcg
    14761 cgccatccat ccaggcgccg ccgcgcttgt tggcgcgggc gtagaggtcg aagaagaagc
    14821 gcccgacgtg cgcgccgttc tccttgatct cgaacagacg aacatccggg tgccagctgt
    14881 cgaagccgct caattcctcg atctggatgc cgtagagctt ttcgacgatg gcgaacaggc
    14941 cgctgagcac cttgtcgatg gggaagtagg cgcgcaggat ttcctgggaa atgctgtagc
    15001 gctgctcgcg cagcttttca ctgtagtagc ccacatccca gctttgcaga tcgttgcagc
    15061 cctgttcggc ggcgaaggct tgcagctcgc gtaggtcctg ctcggcaaac ggcttgccgc
    15121 gtaccccgag gtcacgcagg aagtggagca cctgatcggt ggtctcggcc atcttgctgg
    15181 ccaggctcag ctcggcgtaa ttgccgaagc ccagtagctt ggccagctcc tgacgcaggg
    15241 cgaggatttc cagcatcacc gggccgttgt cgttcttgcc ggcattcggt ccctgatcgg
    15301 aggcgcgggt gcagtaggcg gcatacacct cttcgcgcag cgcgcgatcg ttggcgtagg
    15361 tcatcaccgc gaagtagctg gggaattcca ggctgatcag ccagccgtcc aggcccttgg
    15421 cttcggcggc ttgcttcatt tgtgccttgg ccgaatcggt caggccatcc aaagcggcct
    15481 cgtcggtgat gtgcttggtc catgcctggg tggcatccag cagttgatta gagaatttgc
    15541 tggccagctc ggagagtttc atctggattt cgccgtagcg tttctgctgc tcggccggca
    15601 ggtcgatacc ggacaggcgg aagtcgcgca gggcgtgttc gagaatggtc ttctgcgcca
    15661 cgtcgaaacc ggcagcctcg gggctctcgg ccagtgcctg atacgcctga aacagcggct
    15721 ggttctggcc gatttcggtc caatattcgg acagcttggg caggcaggcc tcgtaggcgg
    15781 cacgcagctc ggggctgttg cacacggcat tgaggtggct gaccgggctc caagcctggc
    15841 ccaggcgcgc gcccagctca tcaagcgtca gcaccaggcc atcccagctc ggtgtgccag
    15901 actgttgttc aagcagccgg ctcatggccg cgcggttgtc ggcaagaatg ctgtccaccg
    15961 ccggctcgac atgctccgga cggatggcgg cataggatgg cagatcgaag gcttgcagca
    16021 gtgggttgtt ggcagtcacg acaggtcacc tgtaacgaaa tgggtttgca gctgtgaccc
    16081 tggcgggcgc taaaggctcc caggggcagg cttgaatgga cacagggcgc tataaaaccg
    16141 cgcgcatgtc ctgaacatgg gtgccatctt aattacaatc aagctcaggc gcagcttaag
    16201 aggtttctat cgtggcgatt cgtacttacc agcaattcac ccctttgctc ggcccacggg
    16261 cttttgtcga tgcttcagcc gtggttctcg gcgatgtgga aatcggcgcg gacagctcgg
    16321 tctggccgtt agtagtgatc cgtggcgaca tgcacagcat ccgcatcggc gagcgcacca
    16381 gcattcagga cggcagcgtg ctgcacatca cccacgccgg gcccttcaac ccgcgcggtt
    16441 atccgctgaa tatcggcgac gatgtcacca tcgcgcacaa cgtcaccctg catggctgca
    16501 gtatcggcag ccgggtgctg atcggcatgg gcagcatcgt catggacggc gtggtgatcg
    16561 atgacgaggt gatgctcggc gccggcagcc tggtgccgcc gggtagccac ttggaaagcg
    16621 gcttcctcta cctcggtagc ccggcgaaaa aagcccgcga gttgagcgag aaagagcgca
    16681 gctacttccg ctacagcgcc gataactacg tgcgcctcaa agatcagcac ctgatggaag
    16741 gctatgccag ctaacgcgtg accggcacct gttgccagcc accgccgagg gcgcggtaca
    16801 gcgccacgcc ggcctgcagg cgcgccaatt gcagctgcgc gctgtcgtcc tgggcggcgt
    16861 agagggtgcg ttgggtgtca agcagagtca gcaagctatc ggcaccggcg cggtagcgtg
    16921 actcggacag gcggaaggcc agttcggcct gtgccagtac cgcctgttgc gcctgccact
    16981 gcgcctcaat gccagccagg ccgttgagcg ccaactgcac atcggcgaag gcgctgacga
    17041 tgctgctgcg gtacgcggct agcagttcct cacgctgggc cagcgccagg tcgcgactgg
    17101 cggccaggta gccgccgtcg aagattggcg ccagcagcgc tgcacccaac gaatacagtg
    17161 gatcggcaaa tacgtcggca acccttcccg cattaccgcc ggccgtagcc gtcagattca
    17221 gacgtggcag cagcgcggcg cgggcggtct gcacatctgc atcagcagca gccagttgcg
    17281 cctcggcgcg ggccagatcg gggcggcgca gcagcaggtc gctgggcagc ccggcgctga
    17341 tcgacggcag ttgcagcgct tccagctgct gttcggccag gttcaggctg gcgctgctgc
    17401 tttgaccgag cagcacggcc agtgccacgc ggctgtcgtc ggtctgctgg cccagcgcag
    17461 ccagagcgcg gcgctgttcg gcgaccacgc cgcgctgctg ggccaactcc agttgggtgg
    17521 cggcaccggc gcgctggcgc gattcgacgg tacgcagcac ccgttcggcg ttgtccaggt
    17581 tgagcccggc gatgcgcagg cgctggtcca ggccgacccg ttgcagccaa ctcgacacca
    17641 ccccggcgct aagggtgacc tgcagcgccg cgcggtcaaa ctggctggct cgcaggtcgg
    17701 ccaatgcact gtcgcggccg gcgtcgaggc ggccccacag atcgacttca tagctcgcag
    17761 ccaggctggc cgaatagcta ttgcccgcca cttcagcatc gccacccagg cgcccttcgc
    17821 ggccggcacc taggttgcca ttgagctgcg gcagcaacgg tgcgccggcc atccgcgcgc
    17881 tggcctcggc ctggcgcacc cgggccacag cggcggccag gtcatcgttg gccactagcg
    17941 cctgatcgac caggcgattc agctcgggat tggcgaaacc ctgccaccag tccggcgcga
    18001 cctgcgtgct tgccagcggc gcgtgttgcc attgcgcagg agcagccgga gtgatcacgg
    18061 caggcggctg gctggtgcag ccgtgcaggg ccagcagcag gggcagggac agcaggcggt
    18121 aactcatcag gcacttcatc ctattccgct gccaacgcga caactgggtc gagcgccgct
    18181 gcggtcttcg ctggcaggta gccgaacagc aggccggtgg ccaccgcgca ggcgaaggcg
    18241 gcgagcatgg cgctcaggga aaagatcagc ggcacatccc agaggatcag cccggcaccg
    18301 accagcaggc cgagcagcac gccgatgctg ccgccgacca gggtcaccag caccgcttca
    18361 ccgaggaact gctggaggat gtcgcgctgg cgtgcaccgg tggccatgcg aatgccgatc
    18421 tcgcgggtgc gttcgcgtac ggtcatcagc atcacgttca tcaccccgat gccgccgacc
    18481 agcagcgaca cggcggcgat caggccgagc atgcgggtca tgctgtcctg ggtgtcggcc
    18541 tcggcttgca ccttggcggc ggcattgtcg acctggaagt cttcgcggcc gtggcgcgcc
    18601 agcagcagtt cgcgaaccgc ttcaccggcg gcattgacct ggctggtcga catcgccgtg
    18661 agggtggcgt attcgggttc gagaatgtcc tggtagaccc gcgcgcggcc ggcctggtag
    18721 gggataaaga ccatctcgtc gtagtagtcg gccccggact ccttgccctt ctcggccatc
    18781 actccgatca cctcgaacgg cgcatcgccg accagcagct gcttgcccag cgggttcggc
    18841 tcgtcgcgga agaactgctg ccgcgccttg tgtccgagta ccaccaccgg cgccagcgac
    18901 tcatcctcgg cggcgttgaa gaagcgcccc agggccagcg gccagtggtg gatgccgggc
    18961 agctgctcgt tgctggaata cacgtagata tcccgcgaga cattgccgta ctgcacggtg
    19021 cgcgggttgc cgatcactgg agtggccagg tcgacttccg gcagcgcggc cacggcgagc
    19081 atatccgcct cgcttatcac tccccagggg ccaccttccg gcggttgcgc ggcgtacagg
    19141 taaagagtgg tcgcacccat cgaggccatc tgcgccatga ctttctgctt ggtgcccagg
    19201 ccgatggcga gcatgacgat caccgaggcc acgccgatca cgatgccgag caacgtcagt
    19261 gcggtgcgga agcgattcag ccacatcacc cgccaggccg agcgcgccac ttcgttgaag
    19321 gtcttccacc agggcaggcg ctggtcatat ccggcggtgg ccatatccgg ggctggcagc
    19381 gcctgggccg agggctgggg ccggccatcc ggagcgccac tgtcgctgac gatttcgcca
    19441 tcgcgaatct cgatcacccg ttgcgcctgg gccgccacgt cgcggtcgtg agtgatcagg
    19501 atcaccgtgt gcccggccgc cgccagttcg cgcaacaacg ccatgacttc ctgaccgctg
    19561 tgcgagtcga gggccccggt gggttcgtcg gcgaggatga tccgcccgcc gttcatcagc
    19621 gcacgggcaa tcgatacccg ttgctgctgg ccaccggaga gctggctggg gcgatggtcg
    19681 aggcgttcgc ccaggcccaa ccgactgagc agcgcactgg cgcgctcacg gcgttgttcg
    19741 gcggacattc cggcatacag cgcgggaatc tccacgttct cgcgcgccga ttcggtggcg
    19801 atcaggtggt agccctggaa cacgaagccg aacgcctcgc ggcgcagcca ggccagctgg
    19861 tcggcatcaa actcggcgac atcgcggccg gcaaagcggt agctgcccga ggtgggccgg
    19921 tcgaggcagc cgagcaggtg catcagggtc gacttgccgg aaccggaggc accgacgatg
    19981 gcgacaaact cacccgcgcc aatggccagg ctgaccccgc gcagcaccgc cacttccggc
    20041 gcgccgtcgt ggccgccata acagcggcgg atatcctgca gttcgatcag cggcgtgctg
    20101 cttaccactg gaacctcgcc acgcgggtgt ccggcagctc ggcggtaatc agttgctcgt
    20161 tctctttcag gccgtcgagc acttctacgc gctgacggct gcgcgcgccg atgcgcactt
    20221 cacgcgcctc cgggcggcct ttgccattga ggatgcgcgc cgtgaagacg ccactcttgc
    20281 cttcgaccgc ctgcagcgct tccagcggcg cggtgagtac gtcgcgcgcg gcggcggtga
    20341 taaaggtcac ttgggcggtc atctgtggca tcagttcgcc gtcctggttg tcgacgtcga
    20401 acagcactgt gtactgcacc gccttcatcg ccaccggtgc gccacctaca tcaccggagg
    20461 cggcgggtgg cagcggcggc gaaggcagga tctggcggat cttggcgtgc cagcggcggc
    20521 cgtcgccgcc gagggtggtg aagtacaggc cgaggcccgg cttgagctgg cggatgtcgg
    20581 cttcggagac cttggtccag gcggtcatcg ctgatagatc ggcgatgcgc agtacggtgg
    20641 gcgtctggta ggtggcgttg agggtctggc cctgctttac gtcgacgccg agtacggtgc
    20701 cggcgatagg tgcgtagagg cgggtgtagc tgagctgagc ctcgttaccc ttgagccgtg
    20761 cctggctctc ggcgatctgc gcgcgcagct gtttgaggcg ggcctgggcg gctttcaggc
    20821 tggcggcggc gacctgcacg tcttcctcgc gtgttgcctc gtccaccgcc aggcgttgct
    20881 ggcgctggtg ttgctgctgg gccaggtcga attgcgcctg ctgttcttcg agttgcgcgc
    20941 gcaggccgtc cagcgccgca cggtcggcct cgaccgtggc caggtgcagg ctggcgtcga
    21001 tctcggcgag cagctggccc ttctccacct gatcagccgg ctcgacatgc aggcgggtga
    21061 cctggccgga cacctgcgcg ccgacgtcca ccgactcgcg cggctgcagg gtgccgatgg
    21121 ccactaccgt ggcctcgaca tcgccacggc tgaccgcctc ggtggcaaag ctcgggccgg
    21181 actcgccgcg ccacagccag ccgcacagca gcaacagcac cagcaaggcc ggtaacccac
    21241 ggagcaggat ggaacgggag aggcggttca tcggttactc gttagcgaat acaggcggat
    21301 tacagcgcgg cgcgcagcaa caggggcagg ctgccgagca gcgtgagggc ggccagcagg
    21361 gcggcggtgc gcgggcgata gggcagcagc agaaccagca acagtcccgc cagcgccagc
    21421 cagccgagcc agagcaccac gccgactgcc gcgccccagg ccagcacgca gggccacaat
    21481 gccagggcca gcagcagcca gccggcaatg cgcaggccat gttgccgggc cgctgccttg
    21541 cgcgcccaga cctgttggtg atggcggttc atggccaggc acaggccgct gaggccggcg
    21601 tagcacaggc tgagggccag cccgctggcg agcagcacgt ccatcagcgc acctccacgg
    21661 cgcttgccgt cgcggtctga cggtggcggc tttgggttac ctggctcttg cgccagaccc
    21721 gctgcgccgt ccacaggcag agcaggccga tggcgatggc caccagctcg accccagcgc
    21781 gctcgccgtc gccctgctgc aggtagctga cgaactgctc gccagtcacc agccagttga
    21841 gcagcggcag cgccaggcac agcagcgcgg tggccagcaa ttgttcggtc caggcgcggt
    21901 ttgccggtcg acgggcggcg tggccgaagg tcgctagcca gaccgcgaag aaggcgacga
    21961 tctcccattc ttcacgggcc gcgatgccca gcggaatcag ccggttggcc cagaagaagg
    22021 cgatgcaggc caggctcagc ccggcaatcg tgccgatgtt cagcacctcg atcactcgat
    22081 aaacctgcgg cgtgcgggtg ccgaattcat gcagcgagcg ctgtcgccgc ttaaccataa
    22141 acaggatcgc cccgctggcc agcatcgctg cgccagccag gccacagatg aaatacagcc
    22201 agcgcgcggc atacccgccg aagccagcga agtgcagggt ggccatcacc gattgcacgt
    22261 cctgggccgc gccgccgcgc agctcgccag gggcctgcag gtcgagttgc gcaccgctga
    22321 caccgtcgaa gtgcacgctg ccactgccgt caccgagcag gcggtattca ccacttttct
    22381 cgataaatcg gccaaaggtc accaccgacg cactgctgtc gccagggtgc tcgatgcgga
    22441 taaagctgag cgggcgctgc aactgctgct cactgcgcac cagcaacata tcgagcgggg
    22501 tcactgtggc agcgacgccg gtttcctcgc gcggctcggg ctgggtcagc aggtcggcaa
    22561 aataggcctc gtcgctcggg taatgcacgg cgatgccggc cggcatatac agcgcgtaga
    22621 aggtcgccag gccggtgtag gcgatcatca gctggaacgg caggctcagc accgacaccg
    22681 cattgtgcgc atccagccag ctgcgctggc ccttgccggg gcggaaggtg aagaagtcct
    22741 tgaagatgcg cttgtggatg atcaccccgc tgaccagcgc taccagcatg gccatggtga
    22801 aaaagccgac aaaccagatg ccggcggtgc cgccatggaa ctcgaagtgc atatgcacaa
    22861 agtgatggcc gccctcgctt tcacgcaggt tggcatcggc cagtggttgg ccgctgagcg
    22921 cgtcgatgcg ctggttgtgg aactgctcgt tggcgtcctg ccagaacagc cgcactgcct
    22981 tgtcgtcgtc attgggcagg gcgatgcccc aggcatgcgc ctgcggctgg tgctcactca
    23041 gataagcctg acccaggcgc accgccaacg gccggtcgac accatcatcc ataccgttaa
    23101 gcgaactggc ctgcggcaag tgcggcttca tccagcgggt gatcggatca tcgaacacgc
    23161 cgaggctgcc ggtgaggaag atcgcaaaca gtacccagca ggcccacagc ccaccccagg
    23221 tgtgcagcca ggccatggcc tgacgcaggc cctgcttctc cactgtttga tctagagcct
    23281 gagtcatgca ccgcccccca accaccagcc gagcagcgtc agcaccacgg ccggcagcaa
    23341 caggccgagc caggcgcggc ggttgtcctg caccgcaaag acccagacga tggccgcggt
    23401 atacaccgca aagctggcca gcgaggcggc caggatggcc tggcccttgg gcagtggcag
    23461 gatcgcgccg agaaagatca cggcggccgc ggccagggcg tagccgccga gaatcgcggc
    23521 ggcggtgcgc gacagcaaga taagcgcagg ccggcgggag gaaggcatag gtttcatcag
    23581 caaccgatca tcaaactgga aaagaaaaac cgccggcacg gccagcggca tgacaaaggc
    23641 ggcagcagaa aatatgcatg ccctacattc aggacggata agcccggtaa aaccggcata
    23701 cccagcgcgc tcaagctgtg actgaatgtg tcggcgacgc tgtcgccggt tacctcgaca
    23761 gcgcggcggg cgctatcaga actccatgcg caggccgaga gtggcgttac gcggggtgcc
    23821 gtaatagctg gaggaggtgc tggtgtagta ctcctcgttc atcaggttgt tgaggttgag
    23881 ggtggcagtc agctgctgcg tcagcgggta gcgcgccatc acgtcgacca gggcgtaatc
    23941 gtcctgggta aagcgctgat cccagctcgg acccacgccg tcgctgtaga tctcgctctg
    24001 ccagttgatc ccgccgccca gggtcaggcc gttgccgacg ctgcgcaggg tgtaggtggt
    24061 aaacagcttg gcggtgttct gcgcgatatt ggtgttgagc ttctgccctt cgctgtcctg
    24121 cacgatattg cggctaaagc tggcgctggc ctgccagtcg gtggccagtt cgccggagac
    24181 ctccagctcg aagccacggg ttttggtgcc ggattcggcg cggtaggcct ggctaccgtc
    24241 cggtgcaaac tgccccggaa tggagactgc caggttgtcc tgctcgatct ggtacagcgc
    24301 caggccaaga ttgaggcggt catcaaagaa ctccgccttg ctacccactt cataacccac
    24361 gccttccagc ggctggatat aggcaccgtc caatgtcttt ctgctttgcg gcttgaagat
    24421 gctggtgtag ctgccgtaca acgaccagtt gtcgttgagg tcgtagacca cgccggcaaa
    24481 gggggtgacc acgccggttt cactgcgttg ctcgtcggtg tcaggcgtcg ctggatcgta
    24541 gttgtcgatc ttttccctgt tttcccaatc aatcacgcgg gccccgagga taaacgacag
    24601 gtcttcgctg atacgcaggc gggtgctgcc gtaggcggcg gtttgctctt cactgaagtc
    24661 aatgcggcca taggctggaa gcggtgggcg acctgggttt gagccgtccc aactgtagat
    24721 attgtcgatc tcagggaacg gtcgatgcca ggtgtaggcc ggggtgctgt agtcggtgtg
    24781 ctgccggcta tagccgatga tcgcgtcgtg ctcacggcca aacaggtcgt agctgccggc
    24841 caggttgaag tcgaagctgt tctgcacggg tttggctttc cagcggttgg cgtagaggat
    24901 cacgccgcca ccatctggct gcggattgcc gccgccggca tagccactca gctcgtcgta
    24961 ttcgctgcgg cgctgattga ggcttagttt ggctttccag cgctcggtca ggttgtgctc
    25021 gaggatggcg aagcccgaca cggcgctgcg attggagtaa gcccaggtcg cgccggcgga
    25081 atccgagtcg tcccagtcgg tccgtgagcc gtcttggttg aacaggggca gaccgatgcg
    25141 cgcatggtcg tccacctcgt ttgattgcga gtcgatgccc atggtcagca gggtgtcggg
    25201 ggtgagatcg gcctcgatca caccgtagaa gatcttctta cgctcgttga ggcggtcgat
    25261 ataggactcg ttgtcctggt aggccgcgac cacgcgaccg cgcacgttag cgctgtcggt
    25321 cagcgggccg ctgacatcga tttcggtacg ctggtagtcc cacgaaccgc ccttgaccgt
    25381 gatgctgctc tgcggctgga aggtcgggcg tttgcgaatc atgttcaggg tcgcgcctgg
    25441 ggtgcctacg ccattggtca ggccggtggc accgcggata atttccacgc ggtcatacag
    25501 caccatatcg ttgctctgcg cggggttgct gtagttgctg tcgagcaact gcaagccgtc
    25561 gatctggtag ttctccacgg caaagccgcg tgagtagatg gcgttgttgt cagagccgct
    25621 gctaccggcc tggttaaagg tcaagccggg ggtctggcgc accacctcgg tgagctcggt
    25681 aaggccctga tcgcgcatac gctggctggt gatcaccgag atcgactgcg gggtttcgcg
    25741 gatcgacagc ggcaggccgg tggcggtgct ggtggaacca gtggtgtagg agccgctgcc
    25801 ttcggtggtg tcgcccaggc tctgcgcggt gacggtggtg gcgccgaggt ccagcgagcc
    25861 attgctctgg cgcggcagca gcgagtagct gccattggcc tgttcgaccg cttgcagtgt
    25921 gctgccggcg agtagtgcct gcagcgcctg ccctgcgctg tagcgaccct gcaacccggc
    25981 ggattgctgt ccggcggcgg actctgggat gaacgacacg gtgactccgg cggtggtcga
    26041 gaactgcatc aacgccgctt ccagcgaacc ggcgggaatc gcaaagtcga actcagccgc
    26101 cgttgtttgt gcccaggcgg cgggcgctgt cagcgcggca cccaccgggc tggcgagcgc
    26161 cgcgcccaat aacgcatagc ggatcgcctg gcgcagtggt tgtggggtgg cggtggcttg
    26221 cgagcgcgga gcgggggtgt gcgacttgca ggagaacatg aactttcctt tcgcccagtg
    26281 gcaggtaatg acagcgtcta tatgcgcctc tacctaccaa gacgaatgag aatttaaatc
    26341 aacaacccct gtgaatgaat tttctttgtt taacgcgctt ccagctgcgc ccagtagcgg
    26401 gtgaagcgat tgacccgcac cggcagcgtg gtcgccaggt tatccagcac gccgtcgata
    26461 tctgtcagct ggaatgcccc ggagatgcgc agcccagcca ctgcgggggt tgcccgaaga
    26521 tggccggggc gataacgcga cagctcggcg agaaagtccg ccagccgcca gtcactgacc
    26581 accagcagac cgcggctcca ggcatcggcg taaggcggcg ctagttctgg cgcgccggcg
    26641 tgctgtgcgg aaaagctcaa acgctggccg ctggcgacac gcactgtctg ctcgggaagt
    26701 tcagctggac gcacttccac cgcatgttgc agcacatcga cataagtccg ccggccttcg
    26761 ctgcgcacca gaaaacgggt gccgagggcg cggatgctgc cttgcgcgct gtgcacgata
    26821 aaaggtcgag ccaggctgtc cttggccgtt tcgatgagga tctgcccgct gtgcagctgc
    26881 acttcgcgta gcgtgttgct gtagcgcaca tccactgcgc tgtgggtatc cagggtcagc
    26941 tggctgccgt cgtccaggcg caggctgcgc cgctcgccgg gcgcggtgcg gtagtcggcc
    27001 agcagctgcg gccagggcgc agcctgccaa accagcgcgc tgctgccgct ggccatcagc
    27061 agcacgctca gcaccttgag ggtgtcacgg cggttgctgc gcatgccgct caacaggggc
    27121 ctggcgaccg caggctctac tttgccgagg gtctgctcaa gtttctgcac ccgcgcccag
    27181 gcctgacggt ggagcgggtc gcgttccagc cattgctgat gcgcctgctg caggggtgtg
    27241 tgcaggccgg catcgcgcag gtcgacgtac cactgggcgg cggcttccag caccttgaga
    27301 tcgagcgatg cagccattgt gcttaatcct cgatcagtac caggcagtgg gtcatggcac
    27361 gcacgaagtg cttgcgcacg gtgttggttg aaagcgccag gcgtctgccg atttccacga
    27421 agctcagccc gtcgagttgc gccagcagaa agatctcgcg cgcacggccg cccaagccat
    27481 cgagcagggt gtcaatttcc accagggttt cgacgataat ggcgcgcgtt tccggcgata
    27541 catcatgttg ctcgggctgg ctcgccaggg tgtcgagata cgcctgctcg aggctgcgcc
    27601 gacggaacat gtcgatcatc aggctgcgcg caatggtgct caggtaggcg cggggctcgc
    27661 gcagttccgg gttatcgcgt tggctgatca ggcgcacaaa ggtgtcgtgg gccaggtcgg
    27721 aggccttgtc ggcgcagccc aggcgcttgc gcaaccagcc ctgcagccag ccattgtggt
    27781 catggtagag ctggtgcacc ctgtgttgcg gggcgagatc aggggtcgac atgcgtgggc
    27841 accggcagcg cgcattgcgc gaatgagaat ttgtatcatt ttgcctgctt tcttcgccca
    27901 cccgcaaccg gatacaccct gcgcgccaca ccggccgtct tgttcctctt agcctgtgac
    27961 atagccatgc actaccagaa catcctgttt gacctcgatg gcaccctcac cgacccgcgt
    28021 gaggggatta cccgttcagt gcagcatgcg ctggccaaac tgggtatcga cgagcctgat
    28081 ctgcaggcgc tggagcactt tatcggcccg ccgctgttgc agtgttttat gcagacgtat
    28141 cagttcgacg aggctacggc ctggcaggcg gtgaaccatt accgcgagcg tttccgtgag
    28201 gtgggtctgt atgagaatca gctgttcgat ggcgtgggcg agctgttgca actgctgcag
    28261 agccagcagc gcacgctgta tatcgccacc agcaaaccga cggtatttgc cgagcagatt
    28321 gcccggcact ttggctttgc ccagcacttt aaggtgatct acggcagcga gctggatggc
    28381 acgcgcaccg acaaggtcga gctgatcgcc catctgatgg aaaaggaaca gcttgcgcca
    28441 gaggcgacgc tgatgatcgg tgatcgcaag cacgatctga tcggcgccag gcgcaatggc
    28501 ctggatgcgg cagcggtggg gtatggtttt ggcagcttgg ccgagctgca ggccgaggcg
    28561 ccgacctatc acttccacag cctggccgag ttgcaccagg cgtttgtggg ctaggccttt
    28621 atctgcgggg ggttagccgt ttttctgata gatgatcttc ttggtgccgc cgtcgcagct
    28681 accgaccacc atattggggt cctgagcttc ttcgttgggc acgatttcta gggtgtagct
    28741 ggccacgccg gcggcctgga ttttcacttc aatctccgca cgcagctctt cgcagggctt
    28801 gggtgcggcc aatacgctgg tgctgagtag gccaagcagc acggccaggg tgaatttgtt
    28861 cattgcatcg ctccttttgc gtgaacagaa actaccaatc ttggagtggg gtttaggtac
    28921 gtaagttcta agagcccgca gataactcag ccaaagcctg atgccgctcg gtttccggca
    28981 gtttgccgag ggctgcgacg cgctcataga aacgcggcca gtcgcgctca acctgctcaa
    29041 acagcgcggc aaaggccggc acccattgat catagaggcc aaacggcaac agcttggcgt
    29101 tgttgatcgg cgcgttgatc cagtcgtcgt aacgcccgct gccgccccac tcgcgctcgc
    29161 ttagcgcgtg gtattcggcg cgcaggcggg cgatctcggc ctgcttgccg ctgcgcattt
    29221 gcgtgggcgg caggtcgctg tcatacagtt gctgcagccg cgcccggctg gccatgacca
    29281 gggcgataaa ctgctcacgc tgctgttcgc cctgcgggtc gctgggctgc aggccctgtt
    29341 tggcgcgcca ttggcgcagg ccttcacgtt cgacaaagct ggcgaaggac tcgttaaacg
    29401 cagtgtcgtc cgccacatac aactgctggt gcgccagctc atggaagatc accgccacca
    29461 ggcgttcatc gctccagcgc agcatggtgt tgaggatcgg atcatcgaac cagcccaggg
    29521 ttgaataggc ctcgacgccg cccaggtaga tatccaggcc ctgctgtttc agcagcgctg
    29581 ccgagccgcg cgcgcgaccc tggctgtaga agccgcgata ggccacgcag ccggcgatgg
    29641 ggaaacagtg cagctctggc gacagggaaa actccggggt ggcgaataca ttccacacca
    29701 caaaaggccg ctggatatcg gcgtacaggc ggtagctatc gttgcttggc aggctcagct
    29761 gcgcgctggc gaaatcccgc gcctgctgag ccagagccag gcgctgcttc agctcggggg
    29821 cggccgccgg gttctctagt agctcggcaa taggctcgcg ggcctgcagc agttgcagat
    29881 gtccgccggc cagatggctg taatagtcca gggtgctgca accgctgagc agcagcgcgg
    29941 cggccagcgg aacccagcgc caaggcaagg catcgaacat gggtcagtac cggcgagtaa
    30001 acaggctgcc acgctagcgc atcggcgcta ctgcctccag cgcaggatcg gcgtaggcta
    30061 acggttattg cgatttgtgg aggtgcccca tgcgttattt ggcttttctt ggcgctgtgc
    30121 tggtgctcag tgcctgcgca tcacccgtgc catcgcctga cccgaaccag gcctgggtcg
    30181 agctgcgcag tcatgccggc acgctgctga tggcggaccg gcttgatggc cagcgtttga
    30241 acgatggtcg ctattttcag gtgcccgctg gtgcccatga gttgcaggcg cgctttcagt
    30301 tcgaagtgaa cggcggcggt ggtctggatg gcacttccga accgcgccag gtgacctgcg
    30361 aaattcgcgt gcgctacgac aattttctgg ccggccagcg ctaccggctg gaggccagac
    30421 ccatgcagat gaaagctcag gcctggctct acgatgggca gcgcaatgtg ctggcgcggg
    30481 gcaagatact gcgctgcggc acgttctaaa gcgcgttgcg atcagctatc gagcaccgcc
    30541 tccagggtga tctcggcgtt cagcaccttg gacaccgggc agccgttctt ggcggtttcc
    30601 acggctttct cgaaggccgc gcggtcggcg ccgggaatcc gcgccttgag tgtcaggtgc
    30661 acggcggtga tggcaaagcc gccatcctgc ttgtcgaggg tcacctctgc ttgcgtgtcg
    30721 atgctttcgg cggtcatccc ggcctcgcca agttccttgg acagggccat ggaaaaacaa
    30781 ccggcgtgag cggcgccaag cagctcttcc gggttggtgc ccggctgatc ctcgaagcgg
    30841 gtattgaagc cgtagggcgc gcttttcagg gcgccgctct gggtcgagat ggtgccttta
    30901 ccgtccttga tgccaccttg ccagtgagct gaggctgtct tcttcatccg aatactccag
    30961 tgcgtgggtt atagggttga gagcaggctc gggcggcaca agttcgaaaa acttctagtg
    31021 caattgcgcc accagctcga acgcatgcag acggtcggca aagtcataca ggtcgcaggt
    31081 gaagatcagc tcatcagcgc cggtctgttc cagcaacacc ttcaggcgcg cacgaacctt
    31141 gtccgggcca ccgatcatcg ccatgccgag gaagctgctc accgcctctt tttcatgcgg
    31201 cagccacaga ccctgcatgc tcgccaccgg cggtttctgc accaggctct ggccgcgaat
    31261 cagcgcgaga atgcgctggt aggccgaggt ggccaggtat tcggcctgct catccgtgtc
    31321 ggcggcaatc agtggtacgc cgagcatcac atagggcttg tcgagcaccg ccgagggctt
    31381 gaagtgattg cggtagacgc ggatcgcctc atgcatatag cgcggcgcga agtgcgaggc
    31441 gaaggcgtag ggcaagccct tctcaccggc cagctgagcg ctgaacaggc tggagccgag
    31501 cagccagatc ggcacgttgg tgtcagtgcc gggcatggcg atcacccgtt gatccggtgt
    31561 gcgtgggccg aggtagcgct gcagttcttc gacatctgcg gggaagtcgt cggcgttgcc
    31621 aatccggtcg cggcgcagcg cctgcgcggt gaactgatcg gcgccaggtg cgcggcccag
    31681 gcccagctcg atacggccgg gataaagcgt ggccagggtg ccgaactgct cggcaattac
    31741 cagcggcgcg tggttgggca gcatcactcc gcctgagccc aggcgaatcg tggacgtgcc
    31801 tgcagccaga tagccgagca gcacggcggt ggccgaactg gcgatgccat ccatattgtg
    31861 gtgttcggcc acccagaagc gctcaaagcc gagacgctcg acatgctggg ccagcgccag
    31921 ggagttatgc agcgcctggg cggcgttgcc gtcatcacgc accggggcga gatcgagggt
    31981 ggaaaatttg ctctgtgcga gtgcgctcat cgggcctgcc tttgattatc cgtttgcttg
    32041 ggcgcaacaa cgcgccaata cctgagttag atagtggggt ttgttctgtg gattccaagg
    32101 ggacgatgcg gcataccaat gcagatgccc agcaatcggg ccgcggccac gattgctggg
    32161 tgccggtcag ctattgctca ggcggaagcc gattttcagg gtgacttggt agtggccgac
    32221 cttgccatcc acgatatgcc cgcgggtttc cgtcacttcg aaccattcca gattgcgtat
    32281 ggatttggcc gcttcggcca gggcgttgtt gatcgcctcc tcgatactgg tcttcgagga
    32341 accgacgatt tcgattttct tgtacgtatg gtgatcggac attgcagctc tcccaggtgg
    32401 ctgaataggt gggtcatgta gttgagtccc acccctgaac gctaaagttc agattagtcg
    32461 cacttgctgg gcacggttgc tggtgagcgt actaagtcgg taagatgaga ataattcaca
    32521 attaccatcg aggcgcccag ccgtgcgcag ccccacgacc agcaagctgg gattcttctt
    32581 cagtgaccac caccgctggc ttctgcaaca tatccagcgc cgcttgcgta atcgcgcaga
    32641 cgccgaggac acggccgccg agaccttctg tcagatgctc gcctccagcg ttgatcccga
    32701 cagcatcgaa caaccgcgcg cctacctgtc gaccatcgcc cgccggctga tcttcgaccg
    32761 ccatcgccgt cgccagctgg aactggccta cctcgaacgc ctggccagct tgcccgaggc
    32821 attggcccct tcaccggaag aaatcgccct gctggtcgaa gcccttagcg ccatcgaccg
    32881 cacgctcgcc ggcctgccgc aggtggtgaa aaaagccttc ctttacagcc agctcgacgg
    32941 cttcagttac gtggaaattg gccgccgcct cggcgtttcc gaacgcactg tgggccgcta
    33001 tatgacccag gcgttgcagc agtgctacct gacggggatg cagccatgag ccaggcagta
    33061 ttgacccgcg cgggcgaaga ggccatcggc tggatggtgc gcctgcgcgc cgccacgccc
    33121 gatgcgcagt tgcaggcggg tttcgacgcc tggatggtta gcgatcccgc gcatgccgct
    33181 gcctggaagc gcctgcagca ggggcttggt ggtcactacg agaccttgcg tggtttcgag
    33241 cggcgcgctc ctggccaggc ccgcgaggta ttactgcggc ctgatacctc gcgccgcgaa
    33301 gtgctgcgcg gcctggtcgg gctcggtctg ttcggcggtg gattgtggct ggcggggcgc
    33361 agtcagcccg gtcaggcgct gctcgccgat ctgcacaccg gcactggcga gcggcgggct
    33421 tacagcctgg ccgatggtag ccagctcaat ctaaatgccg gcagcgcggt cgacctcgat
    33481 ttcacggccc gccagcgtct gttgcgtctg cgccacggcg agctggtggt gcaggtcgcc
    33541 cccgacccga cgcggccgtt tattgtgcgc agcgcccagg gcgatgtacg ggcgctgggt
    33601 acgcgctttt tggtacgtca ggaagagcag gccacccgtg tgctggtgct tgagcattcg
    33661 gtgcgcctta ccttggcaaa cggcaccacc caggagctgc aggaaggcca ggcggcattg
    33721 ctgcacgcgc ggcatattgc attgctgggc cgcgctgaac agcaccgcgc cgactggctg
    33781 caagggcagc tgagcgtgct tgatgagccg ctggaggcgg tgattgaggc gctacggcct
    33841 taccgcgcag ggctgattcg cgtcgctccg gcagcccgcg ggttgcgcgt gcagggtgtc
    33901 tatcccctgg ataaccccga gcgagtactg accgccctgg gcgaaaccct gccgatccgc
    33961 atcagtcatt tcgggccgtg gctgacgctg atcgatgcgc aacactgatg ccgagcgtcg
    34021 ggggcctgcg agcgtttcac cggcgctgta aatttttatg aaaaatattc tcatccgtgt
    34081 ccggttttca tctttcgtcc cacctatctc ctgacatcac cattcttatc aggagcattc
    34141 cgtgcccaaa ccctggcccc gcgcccttgc catcgccatt gcgctcgccg ctgttggccc
    34201 gttcggctac gcccatagcg agcctgccac ccagcagcag acccagcgtt tcgacctgcc
    34261 cgccgccagc ctggctgaga ccatcaacgc catcgcccgc cagagcggcc aggtggtatc
    34321 gctggaacca gggctggtgc gcagcaagca ggcccccgcg atacgcggtg agctgacggc
    34381 cacccaggcc ctgcaacagg ctttgagcgg cagtggtctg gagctgcgga tgaccgagag
    34441 cggtagcttc agcgtgcaaa ccaagccaca aatcgacggt gcgctggagc tggacgccac
    34501 taccgtgacc ggtcaggttg tgggcgcgac caccgaaggc agcggctcct acgcggcgcg
    34561 ggcggtgacc atcggcaagg gcgagcactc gctcaaggaa attccgcagt cgatcagcgt
    34621 gatcactcgc cagcagcttg atgatcaggg catcaccgac ctgcgtgacg cggtcaacca
    34681 gaccaccggt ctggtcggcg ccaccggcat cggtccgggc atggtgattt cctcgcgtgg
    34741 cttccagatc gatgattggc agtacgacgg cgtgccggtc ccgcgtaaca cctatgtgct
    34801 gggtaactgg gcgactcagg acctggtgtt ctttgaccgt gtggagattc tgcgcggcgc
    34861 ttccggcctg ctgcagggca ccggtagccc aggcggggcg atcaacctgg tgcgcaagcg
    34921 tgggcagaac gtgccgactg ccagctttac cggcaaggcc ggctcgtggg atcactacgg
    34981 cctgcaggcc gatgccggcg gcccgttgaa tgacagcggc agcatccgtg ggcgcatggt
    35041 ggctgatcag gacgacagcc actctttcac cgattacgtg tggagcaaga ccaattcgct
    35101 gtatggcgca ctggacttcg acctgagtct ggacaccacc ctgggcgtcg gcataagcaa
    35161 ttccgacctc gattcgcgtc ccatgatgcg tggcctgccg cgctaccccg atggccgcga
    35221 catcggcttc tcgcgctcga cctacagcgg ggccagctgg aaccgcaacg acattgagca
    35281 gaccacggtc tacgccgacc tcgaacaccg cttcgatgac aactggcgct tcaagactgc
    35341 ggccctgcgc atgcgcgaac acaacgactc gctgcatcag cgcatgcatg gcaacgttgc
    35401 cgccgatggc agtggcctga acttcgccga ctggatcacc aacttcgagt cgaccaagat
    35461 cggcctggac atgttcctca acggcaaatt cgaggcgctc tccctagagc aggagctgac
    35521 cgtgggcggc aactacgcca agtacacatc ggacgatttc tacgcgcgcc gctttgtgcc
    35581 tggcggcaat atcttcgaca ttgatcatca tcgccccgag ccaacgctga acgacatcct
    35641 cgcctcaccg ggcggccgcg ccaccaatgc cggttatgac gtgcggcaga aggggttgta
    35701 cggcagctgg cgggtcaagc tggcggagcc gctgactgct gtggtcggcg ctcgcgtcag
    35761 ttggtacgac tacgcctata tgctgccgga tgagaactac aaggacagca tgagcgagac
    35821 tggcgaggtc actccctatc tgggcctggt ctacgacctg agtcaggagt gggcggttta
    35881 cgccagctac accgatgtgt tcgaaccgca gaacgcgcgc actgcccagc agcagatgct
    35941 cgaaccggtg atcggcagca actatgaagt gggcctcaag ggcgaactgt tcgatggtcg
    36001 ggtcaacact tcgctggctg tattccgcta cgaccagaaa aaccgtgcgg tgaccgatga
    36061 ggcttcgggc tttgcctgtg acggttggta ttgctcgacg gcctccggca aggtgcgcag
    36121 ccagggcatc gaagccgaaa tcagcggtga agtgctgacc gacctgcagc tgttcgccgg
    36181 ctacacctac aacaccacca agtttattga cgacccggaa aacgagggca gcatctacag
    36241 ccagtggacg cccaagcaca tgctgcgtat gtgggccgac taccggttgc cgggcgactg
    36301 gagccgtgtc agcgtcggtg gtggcttcac ctcgcagagc cacactgtca gcttcgaccg
    36361 ggaattcgat gtgccgggtt tcaccgtatg gaatgcccgc ttgggctatc gcctcagcga
    36421 ggaaatcgac ctggccgtca acgccaacaa cctgttcgac aagcgctact acctgcctgg
    36481 ctatgccgcc gccgatggca gcaacaactt cggcgagccg cgcaatctga tgctgagcgt
    36541 caagtacacg ccagcgctct gacttggttg ggtggcagct gcctaacgac tgccacccac
    36601 atcttgtccc ggcgtatcca gctccttgcg cagccactgc gccaggcgtt cggcgcggtt
    36661 atcgcggttg cgtatcggta cccaaagcgc caggcgtgcc gaggtttcga caaagcccca
    36721 gggcgcgacc aggcgaccgg cggcgaggtc gtcggcgacc agttgctgcg gtgcgatggc
    36781 cacgccgagg ccggcggccg cggcttccag caggtagtaa aggtgctcga agccctggcc
    36841 gagttttagc gctgcggcgt ccagggcatt gctagccgcc cagctgggcc aggcttgggg
    36901 gcgggaactg gtgtggagca aaggctcgcc cagcagagcg gcgggcggag ccaggtgtaa
    36961 ttcggcgaag cgcgcataac gcgggctaag caccgggccg atgcgctcgg cagccagctc
    37021 gaacacctgc atatccgctg gccacggcgg ctcggcgaac cacagggtgg catccacgcc
    37081 agggcggcgc gggtccagat cgccttcgct ggcggacagc tgcagacgca gttcaggcag
    37141 ctcgcggtta agccggtcca ggcgcgggat aaaccagcgc gccagcaggc tgcctgggca
    37201 ggccagaaca aacggcgcat cggcctgccc ctgctgcagc tcggcgcagg tgctgcgcag
    37261 gcggctgaac agatcggcgc tgatatcgcg cagacgaatg cctgcatctg tgagtttaag
    37321 gccgcgacct tccttgctaa atagcgccac acccaggtgt tcctccaggg cgcgaacctg
    37381 ccgactgacc gcaccatggg ttacgtgcag ttcattagcg gcttggctga cactgttcag
    37441 ccgcgcggcc gcttcgaagg cgcgcagggc attaaggggt ggaaggtcct gactcatctg
    37501 tgagttttcc tgacaggttg cggcgatctt atcgcttttg ccgcgcccgc cacagcttta
    37561 tcctgtgttc attgccgtag aagcggggac cctacccgtg aagcgcgccc gaattttctg
    37621 aggagaacgc catgacttcc ttccgcactg gccctgacgc caacggcctg ttcggctcgt
    37681 tcggtggcca atatgtcgcc gaaaccctga tgccgctgat ccacgatctg gccgaggaat
    37741 acgagaaggc caagctcgat cccgagttcc agaaggaact ggcctacttc cagcgtgatt
    37801 tcgtcggccg cccgagcccg ctgtacttcg ctgagcgtct gaccgatatg tgcggcggcg
    37861 cgaagatcta cttcaagcgc gaagagctca atcacaccgg cgcgcacaag atcaacaact
    37921 gcatcggcca ggccctgctg gcccggcgca tgggcaagaa acgcatcatc gccgagaccg
    37981 gcgccggcat gcacggcgtg gccaccgcca ccgtggccgc gcgcttcggc atggagtgcg
    38041 tgatctttat gggcaccact gacattgatc gccaacaggc caacgtattc cgcatgaagc
    38101 tgctgggtgc caccgtgatc ccggtggtcg ccggcaccgg cacgctcaag gatgcgatga
    38161 acgaagcgct gcgcgactgg gtgaccaacg tgcacaacac cttctacatg atcggcaccg
    38221 tggccggccc gcacccgtac ccagcgatgg tgcgcgactt ccaggcggtg atcggcaagg
    38281 aaacccgcga gcaaatcctc gcccaggaag gccgcctgcc cgacagcctg gtggcctgca
    38341 tcggtggagg ctccaatgcc atcggcctgt tccacccctt ccttgatgac aaagatgtgc
    38401 agatcgtcgg cgtggaagcg gccggctacg gcatcgaaac cggtaagcac gccgccagcc
    38461 tcaacggcgg cgtacccggc gtattgcacg gcaaccgcac cttcctgctg cagaacgacg
    38521 acggccagat catcgacgcc cactcgattt ccgccggcct ggattatccc gggattggcc
    38581 ctgagcacgc ctggttgcat gacgtcggcc gggttgaata cacctcggtg accgacgaag
    38641 aagccctggc tgcgttccac acctgttgcc gtcaggaggg cattatcccg gccctggaaa
    38701 gcgcccacgc cctggccgaa gccttcaagc gtgcgccgaa gctgcccaag gatcacctga
    38761 tggtgatcaa cctgtccggc cgtggcgaca aggatatgca gaccgttatg caccacatgg
    38821 aaaacacttc gcaggagaaa cactgatgag ccgcctgcaa acccgctttg ccgaactgaa
    38881 acagcagaat cgcgccgctc tggtgacctt cgttaccgcc ggcgacccga actacgacgc
    38941 ctccctggct atcctcaagg gcctgccggc ggctggcgcc gatgtgatcg aactgggtat
    39001 gccctttacc gaccccatgg ccgatggccc ggcgattcag ctggcgaata tccgtgccct
    39061 ggaaggtggt caggacctag ccatgaccct gcagatggtt cgcgaatttc gtgaaggcga
    39121 gcaggccacg ccgctggtgc tgatgggtta cttcaacccg atccacaaat acggcgtcga
    39181 gcgctttatt gccgaagctg tggcctctgg cgtcgacggc ctgatcgtgg tcgacctgcc
    39241 gccagagcat aacgtcgacc tatgtgatcc ggctcaggtc gcaggcttgg actttatccg
    39301 cctgaccact ccgaccaccg acgacaagcg cctgcccaag gtgctgaacg gcagctccgg
    39361 cttcgtctat tacgtctcgg tagccggtgt gaccggtgcc ggttcggcga ctctggacca
    39421 cgtcgagcag gctgttgccc gcctgcgtcg ccacaccgac ctgcctctgt gcatcggctt
    39481 cggcatccgc accccggaac atgctgcaac tatcgcgcgt ctggccgaag gtgtggtggt
    39541 gggctcggcg ctgatcgacc agatcgccaa tgccagcagt aacgaacagg cggtggatgg
    39601 cgtgctgagt ctgtgtgcgg cgctggctga aggtgtgcgc ggcgcgcgtg gttaatcagc
    39661 cttatgtcgc ccggtagggt ggacatgccg taggcttgtc cacctttcac gcggcggaca
    39721 aggctctgcc atgtccccct acgcagtcac gtagcccgga tgcaatccgg gagcatttat
    39781 tgtcagtaaa acccttcccg gatttcatcc gggctacggg ttcggtgtag ggtgggcggc
    39841 gaaatgtcac ccgcccttgg tttcctgccc cgagtctcgc catgtcacga ccacctatcg
    39901 ctcgcaaacc ccgcgccagt agccagagcc gcatcgccgg cattcttgcc gctgcccgtg
    39961 agctgctggc tgagcagggt gtggccagtc tgtcgatcta cagcgtggcc gagcgggcgc
    40021 aaattccacc gtcctcggtg tatcacttct tcgccagtgt gtcggccttg ctggaagggc
    40081 tgacggcaga tatccacggc gcgtttcgtg cctgtctgca ggagccaatt gagcatgatc
    40141 agttgcgtga ctggcgtgat ctgtcgcgtt tagtggagca gcgcatgctg gcgatctatg
    40201 ccgacgatgc tgccgcccgc cagctgattc tggcgcagca cggcctggcg gaagtgaccc
    40261 atgcggatag gcaacacgac attgagctgg gccagttgat gcagcagttg ttcgaccgcc
    40321 acttcccgtt gccgcaactg ccagaggatg tcgatgtgtt cgccctggcc atggaactag
    40381 gcgaccgcgt gtatgcgcgc tcggtgcagc tgcacggtga aatcaccccg cgtatggctg
    40441 aggaaggtat gcgggtgttc gatgcctatc taggtttgta cctgccaccc catctgccga
    40501 aacgcaattt gtaggagcgg ctgggcggca ttccggcttg cccgcgatgg cttaaaccgc
    40561 tgatcgcggg caagcccgct cctacagtca ctggattcag agcgcctttt caaacacctg
    40621 cgagttgcgc tggtagttat acagcgaggc gcgggcggcg ggcaggcggt cgacgctgct
    40681 gggcataaag ccgcgctcgc ggaaccagtg ggcggtgcgt gtggtgagca cgaacagggt
    40741 tttcaggccc tgggcgcggg cgcgttcctc aatgcgttcg agcagctcgt cgccgcggcc
    40801 accgtggcgg taggctgggt tgaccgccag gcaggccagc tcgccgaaat ccgagtcggc
    40861 aatctggtac agcgcggcgc aggcgatgat cagcccttcg cgttcgacga tgctgaactg
    40921 ctcgatctcg cgctccagca cctcgcgcga gcggcgcacc agaatgccct gatcttccag
    40981 tggggtgatc agctccatca gcccgccgac atcctcgatg gttgcctcgc gcagcgattc
    41041 gaactgctcc tgggccacca gggtgccgtt gccggtgcgg gtgaacagct cgctgagcag
    41101 cgcaccgtct tcggcatagc tgacgatatg gctgcgacgc accccggcct tgcaggcttc
    41161 ggcagcggcg tcgagcagtt cgccctggta gctgctgccc agacgctgca gatgcgccgg
    41221 cacctgttgt gggcgcagct cgcgtaccag cttgccggct tcgtcgagca ggccgcactc
    41281 ggcgccgaac agcagcagct tgtcggcatc caggtcaatg gcggcgcggg tggcgacgtc
    41341 ttcacaagcc aggttgaaga tctccccggt cggcgaataa ccgagcggcg acagcagcac
    41401 aatgctgcgc tcatccagct ggcggttgat gcccttgcgg tcgatgcgcc gcacttcgcc
    41461 ggtgtggtga tagtcgacgc catcgactac gccaatcggc cgcgcggtga caaagttgcc
    41521 gctggtgatg cgcaggcgcg agccctgcat cggcgagcgc gccatgtcca tcgacaggcg
    41581 cgcttcgatg gcgatgcgca gctggccgac ggcgtcgatt acgcattcca gggtcgggct
    41641 gtcggtgatc cgcaggtcgc ggtgatagcg cggggtcagg ccattgctgg ccaggcgcgc
    41701 ctcgatctgc gggcgcgagc catgcaccag taccaggcgc acgccgaggc tgtgcagaag
    41761 caccaggtcg tgaacgatat tgccgaaatt cgggtgggca acgccctcgc ccggcagcat
    41821 caccacaaag gtgcagtcac ggtgggcatt gatatagggc gaagcgtggc ggagccagtt
    41881 gacgtggtcg tgcattgctt gaagaacctg tgtcgaagac ggaacgcatg gattggcgcg
    41941 ggcgcgccag acgaggcagc caggagcggt tttatcgtcg cagttggggc agcttcacgc
    42001 gttctatcct ccggatggct caggttgttt ggcttgcagg cagtaatgct caatcagttg
    42061 ctgcagcagg cgcacagtag gctgcaaacg tgacatttcc aggtattcgc ctggctggtg
    42121 agcgcagtcg atatcgccgg ggccgagcac cagggtttcg cagccaagct gctgaagata
    42181 aggcgcttcg gtggcaaatg ccactgcggc ggcggtatgc ccggtcagac gttcggcaat
    42241 gcgtaccagc tcactgtcgg cgttctgctc gaagggcggc acactgggaa acagcggggc
    42301 gaagtcgatc ttcacctgat gctgctcggc cagcggctgc agcttttggc ggatcgccgc
    42361 gcgcagggct tctggctgca tgccgggcag tgggcgcaag tcgaactcca gcgagcattg
    42421 gccgcagatg cggttggggt tgtcgccgcc gtggatgcag ccgaagttta gagtcggctt
    42481 gggcacgctg aactgcgggt tgttgtactc gcgctgccat tgcgcgcgca agccgcgcag
    42541 ctccagcatc acgtcctgca tggcttccag ggcgctgtgg ccgaggttgg ggtcagagga
    42601 gtggccgctc tgcccaagga tgtcgatgcg ctccatcatg atgcccttgt gcaggcgaat
    42661 cggccgcaag ccggtcggct cgccgattac tgcggcacgg cccagcggcc gcccggcttg
    42721 agccagggcg cgggcgccgg acatcgagct ttcttcatcg caggtggcga ggatcagcag
    42781 cggctgctgg aaacgctgct ccaacaaagg ctgcacggct tcgataatca gggcgaaaaa
    42841 gcccttcatg tcgcaactgc cgaggcccac ccagcggccg tcgacttcgc tgagcttgag
    42901 tgggtcggtc tgccacagcg cggcgtcgaa tggcacggtg tcgctgtgcc cggccagcac
    42961 caggccacca gggccggagc catagctggc gagcaggttg aatttgccgg gggagatttc
    43021 ctgcacgtcg caggcgaaac cgaggtcgcc gagccagcta gacagcagtt cgatcaccgc
    43081 gcggttggac tgatcccaat gcgcctgggt gcagctcacc gacggggcgg cgatcagggc
    43141 ggcgaactgt tctttgaagg aaggcagggg catcggctat ctccacaagc gaggcccatc
    43201 atagggccat cgcttgtgga gataaaaccg ctacgggcgt ttagaggaaa atgccgcgca
    43261 ggatataaaa gaagataatc gacagcgccg cgcccaccgg cagggtgatc agccaggaca
    43321 taaagatctt gccgatcacg cccaggttca gcgcgccgat gccgcgcgcc aggcctacgc
    43381 cgagtaccgc accgaccagg gtgtgggtgg tggacaccgg caggccgata gccgaggcgc
    43441 ccactacggt ggtggcggtt gccagttcgg cagcaaaacc gcggcttggg gtcagctcgg
    43501 tgatttcctt gccgatggtg gcgatcacct tgtagccata ggtggcgagg ccgatgacga
    43561 tacccaccgc gccgagcagc agcacccagc ctggtacggc cgacttggcg ccaacggcca
    43621 tttcgccgcc ggactggatc accccgacaa tcgccgccag cgggccgact gcgttggcca
    43681 cgtcgttgga gccgtgggcg aaggccatgg cgcaagcggt aaagatcatc aatacggcga
    43741 agactttctc cacgctggcg tagtggaagt ctttgtccgc ttcgacgtcg atcttgatcc
    43801 ggctgagcag aatgatgccc agcaacatga tcaggccgcc tacacccagg gaaaggaaga
    43861 aaccttccgt ggtggagagg tccaggccga tatgtttgag gcctttggtc agggtcatga
    43921 tgctgaccat aaaaccggtg gcgaacatat acagcggcac gaagcgcttg gcgttttgga
    43981 acgggtgatc ggtgtcgata atcagcttct gtacgctgac aaacaggccg aaggcgacga
    44041 aaccggacag caccggagac accacccagc tggcgacgat tgggccaacg ccgctccaat
    44101 gtacggcatc catggacacg ccaacggcag caaagccgat taccgcgccg acgatggagt
    44161 gggtggtgga aaccggccag cccttggtcg acgccaccag cagccaggtg ccggccgcca
    44221 gcagcgccga catcatgccc aggaccatca ggtccggggt gatcatcgac gcatcgacga
    44281 tgccgctctt gatggtttcg gttacctcgc caccggctag ataggcgccg gcaaactcga
    44341 agaccatggc gatcaggatc gcctgtttga tggtcagggc tttggagccc actgaggtac
    44401 ccttggcgtt ggccacgtca ttggcgccca caccccaggc catgaaaaag ccgaagagac
    44461 aggcaagcag cagcaatacg aggccgtagt ccgcaatcag agacataaat aattaatccg
    44521 tagattccag aaaggacact ggcgcttagc gcgccagcag ttgttccagt cggttgccga
    44581 cacgttcggc acgg
//
LOCUS       DASRUM010000125        29660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_734192, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000125 DASRUM010000000
VERSION     DASRUM010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 29660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29660
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734192"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFF22_08345"
     CDS             <1..240
                     /locus_tag="VFF22_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019334754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZX16854.1"
                     /translation="WERLRHSMRTQAALAGVNAAVVGLLLAALYQPVWTSAIFKPQDF
                     GLAVVALVALMFWKLPPWLVVVGSGAAGWLLSIVL"
     gene            251..556
                     /locus_tag="VFF22_08350"
     CDS             251..556
                     /locus_tag="VFF22_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZX16855.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPIFGLGIIEELRRHGYGLSAGT
                     LYPMLHGLEKKGYLTSHHERTGRRERRVYEITEQGQVALVEARAKVKELFGELIEGC"
     gene            754..1422
                     /locus_tag="VFF22_08355"
     CDS             754..1422
                     /locus_tag="VFF22_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZX16856.1"
                     /translation="MDKRMIKDLLYEQVARIGKAVSSPKRLELLELLAQGEKTVETLA
                     SELSADIKLTSAHLKVLKEARLVTFRREGKYIIYQLAGSDVAGLWVSLRQVAEEHLLE
                     LKLALGQMVADPAKLSAVSREVLLEQARRGEIFVIDVRPPTEYETAHLPFARSMPVAE
                     IEQRLAELPSDVEIVAYCRGPFCLLSDEAVALLAAKGYRVRKILDGVSEWLAAGLPVE
                     QGKS"
     gene            1419..1997
                     /locus_tag="VFF22_08360"
     CDS             1419..1997
                     /locus_tag="VFF22_08360"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX16857.1"
                     /translation="MSMKGRESGMPDEAYWASFFEAETAIDRLFGTAVLGDVIEFGCG
                     YGSFTVPAARRTTGKVIALDIEPEMVDCVRHKAVTFDLPNIQADVRDFVAHGTGVDSG
                     SQTHAMIFNLLHLEQPISLLREAYRTLQDGGVLSVIHWRSDIPTPRGPSLEIRPTPDQ
                     CRAWIAEAGFHAIESVDLQDCCPFHFGLLARR"
     gene            2022..2378
                     /locus_tag="VFF22_08365"
     CDS             2022..2378
                     /locus_tag="VFF22_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HZX16858.1"
                     /translation="MKTLIIFNHEPYDSTDVTWNGLRLADKLVDAGSEVRIFLMSDAV
                     DMARDACRPPEGYDQDLSLMLKALISRGVTVKVCGTCMARCGIYKNQPYFEGAEKSTM
                     PALAEWVIDSDKVISF"
     gene            2439..3611
                     /locus_tag="VFF22_08370"
     CDS             2439..3611
                     /locus_tag="VFF22_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZX16859.1"
                     /translation="MTESSKQGLSTRTIHGHSFKDAHGSPHLPVYDTTTFAFKSTADL
                     LDVVDGRKPGSLYTRYGLNPSIFAIEETLASLEGAEMAWSFCSGMAAETALFLTHGRE
                     GIVCIGDAYGGTLELLASQLPLLGIKTDLILGRELDQLDALLAAGAKLVFFETPTNPT
                     LELLDIRAIAAKAHAYGARVAVDNTFASPVNQRPLELGADFVVHSATKYLGGHSDLTA
                     GVLMGSKELLMPVWGWRKNLGSMITPATASLLARSLRTLVVRVRQQNESAQRIAEAMD
                     RHPKVSRVYFPGLPEFPGHALAKDQMYGFGGMLTIEVAGNGEDATRVADRLKLFALAP
                     SLGGAESLVTQPCTTTHHGLTTEERARRGISDAMLRLSVGLEDAEDLIADLEQALG"
     gene            3645..4322
                     /locus_tag="VFF22_08375"
     CDS             3645..4322
                     /locus_tag="VFF22_08375"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZX16860.1"
                     /translation="MSPADYDAWYDSPRGRWIGQTEYRLLSNELAPFSGGQILDVGCG
                     TGWFTRQMATLPGQFVTGIDLNAEWLAFARSRDARSTYLQANALALPFADNSFDHVLS
                     VAALCFTTDWHKAVSEIVRVTRRRFAIGMLNRNSLLWREKGRHGGSGAYRGALWLSPE
                     EFGDSLKGLPISNSRMRSAIYLPSGSITARTVERLLPGSVPWGGFMVLTGDKKPVGGF
                     ASLPVSN"
     gene            4452..4553
                     /locus_tag="VFF22_08380"
                     /pseudo
     CDS             4452..4553
                     /locus_tag="VFF22_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015648284.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(4586..7615)
                     /locus_tag="VFF22_08385"
     CDS             complement(4586..7615)
                     /locus_tag="VFF22_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HZX16861.1"
                     /translation="MTTKSERLTVLSDAEQEALYGLPDFDDAQRLEYLALTETELALA
                     SSRPGLHAQVYCILQIGYFKAKHAFFRFDWSEVEHDCAFVLSRYFHGESFEHKPISKH
                     EHYTQREWIADLFGYRPWAAEFLAQLAQQAAQTVRRDVMPGFIAAELIVWLNEHKIIR
                     PGYTTLQELVSEALSAERRRLAGLLSEVLDESAKAALGRLLVRDDTLSQLAALKQDAK
                     DFGWRQMAREREKRATLEPLHRIAKALLPKLGVSQQNLLYYASLANFYTVHDLRNLKA
                     DQTYLYLLCYAWVRYRQLSDNLVDAMAYHMKQLEDESSAGAKQSFVAEQVRRQQDTPQ
                     VGRLLSLYIDDSVPDPTPFGDVRQRAYKIMPRDTLQTTAQRMSVKPVSKLALHWQAVD
                     GLAERIRRHLRPLYVALDLAGTDPGSPWLVALAWAKDVFAKQQRLSQRPLAECPAATL
                     PKRLRPYLLTFDADGKPTDLHADRYEFWLYRQVRKRFQSGELYLDDSLQHRHFSDELV
                     SLDEKAAVLAQIDIPFLRQPLDAQLDALATELRAQWLAFNRELKQGKLTHLEYDKDTQ
                     KLTWRKPKGENQKAREKAFYEQLPFCDVADVFRFVNGQCQFLSALTPLQPRYAKKVAD
                     ADSLMAVIIAQAMNHGNQVMARTSDIPYHVLESSYQQYLRHATLHAANDCISNAIAAL
                     PIFPYYSFDLDALYGAVDGQKFGVERPTVKARHSRKYFGRGKGVVAYTLLCNHVPLNG
                     YLIGAHDYEAHHVFDIWYRNTSDIVPTAITGDMHSVNKANFAILHWFGLRFEPRFTDL
                     GDQLKELYSADDPALYDQCLIRPAGRIDRDLIVSEKPNLDQIVATLGLKEMTQGTLIR
                     KLCTYTAPNPTRRAVFEFDKLIRSIYTLRYLRDPQLERNVHRSQNRIESYHQLRSTIA
                     QVGGKKELTGRTDIEIEISNQCARLIANAVIFYNSAILSRLLMKYEASGNAKAHALLT
                     QISPAAWRHILLNGHYTFQSDGKMIDLDALVAGLELG"
     gene            complement(7612..8205)
                     /locus_tag="VFF22_08390"
     CDS             complement(7612..8205)
                     /locus_tag="VFF22_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZX16862.1"
                     /translation="MLVGYMRVSSDSDRQSTNLQRDALLAVGVDARHLFEDHASGAKD
                     DRAGLARALEFVRPGDVLVVWKLDRLGRSLSHLLAIVTSLKEKQVAFRSLTENLDTTT
                     PSGEFLFQVFGALAQYERALIQERVVAGLAAARKRGRIGGRPQAITGEKLEAIVAALD
                     GGMSKAAVCRNFGVKRTTLIETLARVGWTGSRGASSR"
     gene            8444..8722
                     /locus_tag="VFF22_08395"
     CDS             8444..8722
                     /locus_tag="VFF22_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HZX16863.1"
                     /translation="MSKQAVFTMKLEPELRAEFMAEAEAAHRPASQVLRELMREFVQR
                     QRESREYDEFLRRKVEAGRASMRAGLGRSNDEVEAEFAARRASVASQA"
     gene            8719..9003
                     /locus_tag="VFF22_08400"
     CDS             8719..9003
                     /locus_tag="VFF22_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HZX16864.1"
                     /translation="MRVVWTPEAQQDRADVWDYIAADNPRAAARMDEIFSDAAARLIQ
                     HPMLGKPGKIPGTRELIPHESYRLVYQIDGETVWILTLVHTARLWPPVRD"
     gene            9410..9970
                     /locus_tag="VFF22_08405"
     CDS             9410..9970
                     /locus_tag="VFF22_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011514928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HZX16865.1"
                     /translation="MDTRLFAFVGADIGPWRIVRAETRVGEPLPEAKRLNVVSASELQ
                     SETNAPWILRGITSNERYVMRAEKNEIVAKQQGLARPEATCGALIPIRKNAAWWELTQ
                     DERRSVFEQSKHVQIGLQYLPAVARKLHHCRDLSENEPFDFLNWFEYAPIHEVEFNRL
                     LSELRASEEWKYVDREVDIRLTQAQV"
     gene            10104..10460
                     /locus_tag="VFF22_08410"
     CDS             10104..10460
                     /locus_tag="VFF22_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZX16866.1"
                     /translation="MEMESRIFSVTEYIRPSDGEPIRSVVLETKDSAVVVWHAHPGQE
                     ITAHVHPDGQDTWTVISGEAEYYQGGGKVAHLKAGDIAIAKPGQVHGALNTSPVPFVF
                     VSVVASGNAGFALAEK"
     gene            10900..11337
                     /locus_tag="VFF22_08415"
                     /pseudo
     CDS             10900..11337
                     /locus_tag="VFF22_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236504.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(11379..12446)
                     /locus_tag="VFF22_08420"
     CDS             complement(11379..12446)
                     /locus_tag="VFF22_08420"
                     /inference="COORDINATES: protein motif:HMM:NF023168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HZX16867.1"
                     /translation="MLRARDKLLAAGRNPSIDAVREELGSGSKTTIHRYLKEIEEEEG
                     PATGSRVAVSEAIQDLVGRLAARVNEEAEERVTTAQAKHAEQLSQQQQAVAALKSEAL
                     ATRQQLEQTQRALADEKNAHGKTSEALSRKTLENTQLVQQVADLQERLAAEERHRQSL
                     EEKHKHARQALEHFRESTKEQRDQDQRKHEQQVQYLQAELRTVNETLATKQQEAVHTL
                     QENARLLGDLSRAQGDLHQAQEEVRGLRPLKDELGFAQRRSEELGRRLVEQDAAVQQL
                     STSKEQLQAKVDELLSAKQQLELALATARSSVTAQEQVVASMLERFSTPATGPNLVAN
                     PVEEAGRPGKRESKKSTGTGT"
     gene            complement(12879..13973)
                     /locus_tag="VFF22_08425"
     CDS             complement(12879..13973)
                     /locus_tag="VFF22_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HZX16868.1"
                     /translation="MRGSDTFTESLFSIKKLDDFVPAMHPLRAIRAMVNDALVELEDL
                     FAGMYEDAGKGGRPSIAPQKLLRAMLLQVLYSVRSERLLMEQVQYNLLFRWFIGLSME
                     DSVWVPTVFSKNRQRLIEHDAVVAFFNQVLAQAQRRNWLSKEHFSVDGTLIQAWASHK
                     SFVPKDGDGGGSGGSDFRGRPRSNDTHESKSDPDSRLYRKGKTASEMRFMGHTLMENR
                     NGLIVNAMVTQADGYAERVAAKEMINDVRQANPEAAITLGADKGYDAAEFVGELHRLK
                     VAPHVAQNKSGRRSAVPDEIAQTAGYGASMQCRKRIEQGFGWAKTIGQVRQVMVRGTK
                     KVAQLFVLNMAAYNLVRMRSLGQVRLQGAR"
     gene            complement(14111..14845)
                     /locus_tag="VFF22_08430"
     CDS             complement(14111..14845)
                     /locus_tag="VFF22_08430"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZX16869.1"
                     /translation="MSTQIDAKPKPRPRGPAKRLAKGVKWNNAVGGADEQYQLKKQAV
                     IAEASRTFGRHGYKNVSLDEIAAALNVTKPALYYYFKNKQELIYECHELSMRLGDQVL
                     KDAIASETTGYERIATFIKNYISLLTDEMGAPAILHDFSAMTAADQKKITARRRKFDL
                     ELRQVFEDGVRDGSIAPCDAKLAVFWFMGAVGGISQWFHAEGRLRGDEVADIFIGLLA
                     HGIRPVASGKTGTVARTKSSDRPGSA"
     gene            complement(14940..16091)
                     /locus_tag="VFF22_08435"
     CDS             complement(14940..16091)
                     /locus_tag="VFF22_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008091325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZX16870.1"
                     /translation="MTSVSNAFLSEQEVMIRESARRVASEVVASTAAERDRSSAWPYD
                     EIRAVAEVGFMGMLAPQAYGGAGLSFVEYCLAIEEFAAADGGFATLMHVHNSSAHVLR
                     SFGTEEQMQLYLPELASAQRIGAFLLSEPHAGSDTAAIRTTAKRDGDSYVLNGSKQWI
                     SNGSEAGFALVVAATGAPGARDRFSLFIADPKDPGYECLRIENKLGQHTAHTAQIQLD
                     NLRVPAKNMIGEEGRGYGKALSLLSDGRIAIAALSIGIARAALEAAIRYAKEREAYGK
                     PIAELQAISFDLADMATQIDVARQFMLYAARLTDAKLPSIKEAAMAKLFASEMAEKVC
                     SAALQVHGGYGYVNDFPVERYYRDVRVTKIYEGTSHIQRLIIARQILAG"
     gene            complement(16135..16923)
                     /locus_tag="VFF22_08440"
     CDS             complement(16135..16923)
                     /locus_tag="VFF22_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX16871.1"
                     /translation="MLELNNVEVLYSDVILAVKGISAKVPAGQCVTLLGANGAGKSTT
                     LKAISNLIKTEDGRVTAGAITFDGVDIAGGNPAAQVQRGLVHVMEGRKVLRHMTVEQN
                     LVVGGHMGNGRDAKELLDEVYGRIKRLAALRNRTAGYLSGGEQQLLVIGRALMARPKL
                     VMIDEPSLGLAPLMVEEVYGLLSELKASGLTLLIVEQNTRVALELADYGYVMESGRVV
                     LEGPSVNLKSNEDVREFYLGLTVDGERKSFRDMKHYRRRKRWLG"
     gene            complement(16984..18234)
                     /locus_tag="VFF22_08445"
     CDS             complement(16984..18234)
                     /locus_tag="VFF22_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX16872.1"
                     /translation="MTTKNECLSAFARVARCGALAAGVFGLAGAAQAQTTYNVAGLAD
                     FTGPFADVMKDMTGCRKGVLDWWSDEVGKAQGVALRVKEYDTRYDVAQVASLWPGIKS
                     ELDPVAILGIGGSDAAALQQRLPNDKVPQVLATAGYGFAWKSDSWVFNARATYPHEAA
                     AFYTWLQKKSGSSAPIKVGVISSEVSPAYVDIHKGIEKFAKDNPKILEVVETVYTEAQ
                     PTDLTQQVNRLIRKGATVLQVFNNTASVVATRRALQSLGKTNIPIVMSAHNGLISSGK
                     ALGGLEQMEGNYEVYGMAMPTEDPTTARAFFEKLRTKYKAQVSYTSACIMGMSQGLIV
                     ARAFEQAVKIKGVKDVKGEDVRAALLSTPMPSERSFGVLPNIQYSKDAPFPTEGMAVN
                     IGTVEKGHYKLLEQSVPVPVLSKW"
     gene            complement(18315..19373)
                     /locus_tag="VFF22_08450"
     CDS             complement(18315..19373)
                     /locus_tag="VFF22_08450"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZX16873.1"
                     /translation="MSMDPAGVFATSFDQDQALVRTRSQAVTLGLFVMFMFALPWIAD
                     ARSVAVMTSMLITAVVVIGLQINTGLAGQINLGQAAFMGAGAYATALLATKGGMPFWI
                     ALPVGGVCAALFGLIFGLAAVRIKGFYLALTTIAAQILFHFMVLNLPAAWMGGSNGIT
                     VEPARLFGWALDTDAAVYYLCLVVACIMIAGAYGVARSRHGRIFAAVRDDDVASGMMG
                     IDVVRTKALAFLLGAFYAGIGGGLWAYYVRFVSIDQFTLIHSIWFIAMVIVGGMGSVT
                     GALIGVFVIRFAQEFFTSVGPSLVENFSFLSGDVVFAALNIFLGGIIAGFLIFEPRGL
                     MHRWNILKRSYRMWPYPY"
     gene            complement(19370..20254)
                     /locus_tag="VFF22_08455"
     CDS             complement(19370..20254)
                     /locus_tag="VFF22_08455"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZX16874.1"
                     /translation="MSDFLNYLVNGALSGLLYALIAMGFVVIYRASKVFNFAQGELVV
                     FGGYMVWWTIIQMGMPVWIGLPVAFASAAIFGLMIERIFFARLVGESVFSMVMVTIGL
                     LILIRGIVLVLFGPQVRAFPIIFPLEPIILGDVVISRSLLYGGILTIVIGLGLSWFFN
                     RTRVGLTMTAVAEDHQVAMSMGISVQRSIAFAWMLGAVLSTLGAMVHLSGRSINMMSS
                     DIGLAALPVALLAGLESLAGLLLAGVLVGVIQGLASAYLDPLVGGALGSVFPFIIMLL
                     MLLIRPTGMFGWKTIERV"
     gene            complement(20251..22218)
                     /locus_tag="VFF22_08460"
     CDS             complement(20251..22218)
                     /locus_tag="VFF22_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZX16875.1"
                     /translation="MMLKIETLPQLLRRHADATPERLSQRHKQRGIWREYTFAEVERN
                     VLELALGLHHMGVQRGATVAIIGENEPQHYWAEFAAHALGCKVVSLYPDLTADEVQYL
                     LEDSETVCLFAQDQEQVDKGLDVKGKLALLRHVVYWDDTGMWSYNDPILSTFKSVQQQ
                     GLERDAQDPRLYRSLVAKGSPDDVAVLSYTSGTTGKPKGVVLTHRFLIDNAQRLIHAT
                     DAKPGMEYLSYIAPAWATEQINGISAGLGLPMVVNFPESPEQVLANIRELAVEAMTFA
                     PRQWESMASSVQAHMLDAGPLRRAVYEWGMKVGHAFNVGRLDGKPIGWLDRLQFPLAE
                     ALVLRPLRDQLGLTRLRIASCGGATMAPDVFRMFHAMGVSLRNIYGSTETGLLTCHQS
                     DRFDLETVGHWMQAHPEAGAPLEWRVSSDGELQIRGGSPFLGYYRREGSVESKVKDGW
                     FYTGDAVTKTDRGELVFLERLSDLKRLRSGETFPPQFVETRLRFSPFIKDIMTVGDEG
                     RDFVAALINIDMAVLSRWAEDKRIGFSTFTDLSQRPEIAVLIRQEIARVNRFLPPHAR
                     VQRFANFPKELDPDEGELTRSRKLRREFLEERYGALIQGLYDGSREVHIDIPVTYQDG
                     RRSNFNAQVFIHDADGFSLNTSTEPHRRRHA"
     gene            complement(22215..23012)
                     /locus_tag="VFF22_08465"
     CDS             complement(22215..23012)
                     /locus_tag="VFF22_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX16876.1"
                     /translation="MIATTSVSPDGVVLSARNLLLQFGGIVALNDVSIDVRKDELLAI
                     IGPNGAGKSSLMNCLSGFYRPKKGDITLGGLSVRNMAVHEVAGQGLARTFQGTHIFSG
                     MTVVENLMVGRHMHMRSNLAQSFLYFGAARREEVVHREVVEEIIEFLEIEAIRHAPVG
                     SLGYGLRKRVDLGRALAQDPRILLMDEPMAGMNAEEKEDLARFILDVREAKRIPVVLV
                     EHDMGVVMDLADRIAVLDFGRKIAEGTPKEIQANPAVMKAYLGEGGQ"
     gene            complement(23119..23880)
                     /locus_tag="VFF22_08470"
     CDS             complement(23119..23880)
                     /locus_tag="VFF22_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZX16877.1"
                     /translation="MDIKNTVALVTGAGSGLGLATCKALVDAGAKVVAVDVDDDRLMR
                     EMVPLGDSVRALKTDVANDASVKAAVEFAVNTFGALHVAVNCAGILGPCKTLSKGELF
                     PLDLWNRVIGVNLTGTFNVIRHAALAMSQNDANADGERGVIVNTSSGAAWQGQIGQAA
                     YSASKAAVMGLTLPVARDLAQHGVRVVALAPGLFETGMSAGMPTKVAQNIIEKVILFP
                     NRMGQAPEFSALVRHVVENAYLNATTLSIDGGARL"
     gene            complement(23907..25145)
                     /locus_tag="VFF22_08475"
     CDS             complement(23907..25145)
                     /locus_tag="VFF22_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019649722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZX16878.1"
                     /translation="MEDIYVIGVGMTPFGRHLDKDIKQMTREATKAALVDAGIRQEDL
                     QAAFFGNTSQGHMEGQHMIRGQIALRAMGIGRIPVVNIENACASGSSAFVLACNHVRA
                     GAGDVALAIGAEKMFTPDKQKMFSVFDSAWDLSRATDIREELMKLGRDVAVPEGSTSP
                     RPYSVFMDVYAAFARSHMRTFGTTQRQLAAVSAKNHAHSVHNPLSQYQVSYSIDDVLT
                     APPITYPLTLPMCSPVSDGAAAVIVCNRSALKRLNIDASRAIRVLAAVVQSGSARNEE
                     DVANHCTALAARRAYELAGVGPDDISVAEVHDATAMGEIIQSENLGFCEFGQGGVIAE
                     RGDTRIGGRIPINPSGGLESKGHPVGATGLAQVHELVTQLRAEAGARQVEGARIALAE
                     NGGGLEGIEEAVACISILAR"
     gene            complement(25269..26336)
                     /locus_tag="VFF22_08480"
     CDS             complement(25269..26336)
                     /locus_tag="VFF22_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZX16879.1"
                     /translation="MSHQIASRGSPLKRVKVVEFEGIGPGPLAGLLLTQLGAEVTMVA
                     RPSPGDLPKDLTPREDGLLSRGKRRVVLNLKQEDDRTRALEIVAASDGLIEGNRPGVM
                     ERLGLGPDVCGQLNPRLVYGRMTGWGQDGPLAQAAGHDMNYVALTGLMSLTERPGHPP
                     MLPPTVLGDAAGALGFALGMVSGLLAARESGQGCVVDGAIVDVLAMLSPLVQIISASG
                     GFDPGVPSVFHDSPFYDRYICADGRYITVGAIEPQFYALLLQRLGLTDVVLSDQMNRS
                     TWPALKARMTALFASHPSAHWTQILERTDACYAPVLNLTEAAAHPHNVARGLYRVTPG
                     GDIETARGLRFLALTDRAADV"
     gene            26443..27255
                     /locus_tag="VFF22_08485"
     CDS             26443..27255
                     /locus_tag="VFF22_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZX16880.1"
                     /translation="MKNQAMEREQMPPPAVVVDVRKGAMWIRLNRPEAMNSLTPEVLS
                     GIDQALSDAEGNQDVRAVVLTGTGRAFCAGADLKYVRSQSGSDGPRLFLDSVLATMNR
                     LDSFPKPVIAALNGLALAGGLELVLCCDLVLAARGAKIGDAHANYGLLPGGGGSVRLP
                     RVIGPTRAKYLLFTGEFLSADELVEAGLVNQVVDPDELEAATQALVDKIASKSPLGLR
                     RMKALVDDGLQQPVETALRLELLASEVHSHSADQREGLLAFEEKRAPRFIGQ"
     gene            27271..28497
                     /locus_tag="VFF22_08490"
     CDS             27271..28497
                     /locus_tag="VFF22_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16881.1"
                     /translation="MTHALPLDRLDALRAAQQNAYDRAPAIRDIYERARMTPQDIRSA
                     ADLARLPVTSKDALLDLQRKAPPFGGFLAADEAAIRRIFVSPGPIYEPQLNDDQDGHG
                     FSHGFRRAGIGPQDRVLNTWSYHLVPAGVLLDQGIVATGATVIPCGPGGAEQQAQLIL
                     ELGVTCICASTAFFVTLVNTLEHMGHQLPGAWKVKSALLGGEMGDWMGKRRALETRYG
                     IKIFSAYATGDFGLIGYEETGQEGYAVQPERLVQICDPQTGTPLPLGETGEIVVTTLD
                     RGWPLVRFGTGDVASGSVASADGFVSHISLLKGRVGQAVKAREIFIYPRNVEEVLMQV
                     PHMQRAQVVVTKQEHRETVTLRGVLHPGVDAATVEKALHEIFKQRTRLKLDAVEWISA
                     DDLVIDAPVLVDRKYA"
     gene            28972..>29660
                     /locus_tag="VFF22_08495"
     CDS             28972..>29660
                     /locus_tag="VFF22_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003118403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family resolvase"
                     /protein_id="HZX16882.1"
                     /translation="MTRLAQYLEAAERDNTRRSYASAIRHFEIEWKGLLPSTADAIAR
                     YLADHAATLSINTLRQRLAALSRWHNDQGFPDPTKSALVRQVLKGIRSVHSVPEKRAR
                     PLELAVLQQIDQWLDTAISNAQRSGDQLALLRHTRDRSLMLLGFWRGFRSDELVNLRV
                     ENTEVTPGQGMACYLGRSKSDRQLQGRVFKCPALSRLCPVTAFNGWIGLAGLTEGPVF
                     RKIDRWGHVAE"
BASE COUNT         5767 a         9121 c         8920 g         5852 t
ORIGIN      
        1 tgggagcggc tgcgccatag catgcgcact caagcggcgc tagccggggt taatgctgcc
       61 gtggtcggtc tcctgctggc tgcgctctat cagccggtat ggacgagtgc cattttcaag
      121 ccgcaagact tcgggctggc tgtggtggcc cttgtcgcgc tgatgttctg gaagctcccg
      181 ccgtggctgg ttgtcgttgg cagtggtgcg gcgggatggc ttctgagtat cgtgctgtga
      241 ggccgaagca atgaccgaca aagacctcta cggcggcttg atccgcctgc atatcctgca
      301 ccacgcggcc gaggaaccca ttttcggcct tggcatcatc gaagagctac ggcgtcatgg
      361 ctacgggctg agtgccggga cgctttaccc gatgctgcat ggactggaga agaagggtta
      421 tctgacctcg catcacgaac gcacagggcg gcgggaacgg cgtgtctatg agatcactga
      481 gcaaggacaa gttgcgctgg tcgaggcgcg tgcaaaggtt aaggaactgt tcggggagtt
      541 gattgaggga tgctgagtgt gcggttttta tgtcagctta gggtcatctc agaaaacgga
      601 aaaatcgtac gctgagtgtt ggggtaggac aagcagtcgt gccaccagcc gccatctgaa
      661 tcagttggcg tcgtagatgt cggcatttcc tggattcagt cgcgaaacaa aaacatttca
      721 ccttacattc gtataatcgt tagaatgacg gttatggaca aacgaatgat aaaagacctc
      781 ctctacgagc aggttgcccg catcggcaaa gcggtttcca gtccgaaacg actggagcta
      841 ctggaactgt tggcgcaagg cgagaagacc gttgaaacgc tggcgagcga attgtcggct
      901 gacatcaaac tcaccagcgc gcacctgaaa gtgctgaagg aagcacgcct tgtgactttc
      961 cggcgtgagg gcaaatacat catctatcaa ctggccggca gtgatgtggc gggtctgtgg
     1021 gtgagtctgc gccaagtcgc cgaagagcac ctgctggagc tgaagttggc gctcggacag
     1081 atggtggccg atccggcgaa actctccgct gtcagtcgcg aggtgctgct ggaacaggcg
     1141 cggcgcggcg aaatatttgt catcgatgtg cgtccgccaa cggaatacga aacggctcac
     1201 ctgccgttcg cccggtcgat gccggtggcc gagatcgagc agcgccttgc tgaattgccg
     1261 tccgacgtcg agattgtcgc ctactgccga ggaccgttct gcctgctctc tgacgaggcc
     1321 gtggcgttgc tagcggcgaa aggctatcga gtgcgcaaga tccttgatgg cgtcagcgaa
     1381 tggttggcgg caggtcttcc ggtggagcaa ggcaaatcat gagcatgaag ggacgtgaaa
     1441 gcggcatgcc cgacgaagcc tactgggcga gttttttcga ggcagagact gctattgaca
     1501 gactctttgg cactgcggtg ctgggggatg tgatcgaatt cggctgcgga tacggttcgt
     1561 tcactgtgcc ggccgctcga cgcacgacgg gaaaggtcat agcgctggat attgagcccg
     1621 aaatggtcga ctgtgtacgt cacaaggccg tcactttcga tctccccaac attcaagccg
     1681 acgttcgaga tttcgttgca cacggcacgg gcgtggactc cgggtcgcaa acgcacgcga
     1741 tgattttcaa cctgctgcac ctggaacagc ctatttctct gctgcgcgaa gcctaccgga
     1801 ccttgcagga cggtggcgtg ctttcagtga ttcactggcg cagtgacata cccacaccac
     1861 gcggtccttc gctggagatc cgtccgaccc cggaccaatg ccgggcgtgg atagccgagg
     1921 ccggattcca cgccattgaa tcggttgatt tgcaagattg ctgtcccttt cacttcggcc
     1981 tgctggccag gcgttgatcc acctctcctt ggaaaccgtc catgaaaacc ctgatcattt
     2041 tcaatcacga accctacgat agtaccgatg tgacctggaa cgggctgcga ctggccgaca
     2101 aactggtcga tgctggaagt gaagttcgca ttttcctgat gagtgatgcg gtggacatgg
     2161 cacgcgatgc ctgccgtcca ccggaaggct atgaccaaga cctttcattg atgctgaagg
     2221 cgctcatcag ccgtggcgtg accgtcaagg tgtgcggaac ctgcatggcg cgctgcggca
     2281 tttacaagaa ccagccgtat ttcgagggtg ccgaaaaatc caccatgccg gcgctggcgg
     2341 aatgggtcat cgatagcgac aaagtaatct cgttctgaaa cccccgagtt gggcatcgtg
     2401 ccgtttgaaa ccgcagtaag aataaccagg agactcaaat gactgaaagc agcaagcaag
     2461 gcttgtccac tcgtaccatt cacggccaca gtttcaagga cgcgcatggc agtccgcacc
     2521 ttccggtgta cgacaccacc acctttgcgt tcaaatcgac agcagacctg ctcgatgtcg
     2581 ttgacggccg caaaccggga agtctctata cgcgctacgg cctgaaccca tcaatctttg
     2641 ccattgaaga aacgctcgcc agccttgaag gcgcggagat ggcctggtct ttttgttctg
     2701 ggatggccgc cgagacggct ttgttcctga cgcatgggcg tgagggcatc gtttgcatcg
     2761 gtgatgctta cggcggaacg ctggaactgc tggcgagcca gttgccgttg ctggggatca
     2821 agaccgattt gattcttggc cgcgaactgg atcaactgga cgctttgctg gccgctggcg
     2881 cgaagctggt atttttcgag acgcccacta atcccaccct cgaattgctc gacattcggg
     2941 ccatcgcagc gaaggcacat gcctacggcg cgcgagtcgc ggtcgacaac acgttcgcct
     3001 caccggtcaa tcagcgcccg ctcgaattgg gagcggattt cgtcgtgcac agcgccacaa
     3061 aatatctcgg cggacacagc gacctcaccg ctggcgtctt gatggggtcg aaggaattgc
     3121 tgatgccggt atggggttgg cgcaagaatc tcggctcgat gatcaccccg gccacggcgt
     3181 cattgctcgc tcgcagcctg cgtaccctgg tcgtgcgcgt gcgtcagcaa aacgagtcgg
     3241 cccagcgcat cgccgaagcc atggaccgcc accccaaggt cagccgtgtc tactttcccg
     3301 gtctgcccga atttccgggg catgcgctgg ccaaagacca aatgtatgga ttcgggggca
     3361 tgctgacgat cgaggtcgcg ggcaacggag aagacgccac acgcgtcgct gaccggttga
     3421 agctcttcgc cttggcgccc agccttgggg gtgcggaaag cttggtaacc cagccgtgta
     3481 caaccaccca ccacggatta acgacggaag agcgtgcccg tcgcggcatc tcggacgcca
     3541 tgctgcgtct gtcggtcggc ttggaggatg ctgaggattt gatcgcggat cttgagcaag
     3601 ctttaggtta agccactcga tctgtaccga tagcgggttc tgctttgagt cctgccgatt
     3661 acgatgcttg gtatgacagc cctcgtgggc gctggatcgg ccaaaccgag tatcgcctgc
     3721 tatcgaacga actggctcct ttttcggggg gccagatact cgatgtcgga tgcggaacgg
     3781 gttggttcac ccggcaaatg gctaccctgc cgggccaatt tgttacaggt attgatctca
     3841 atgcggaatg gctggcattt gcgcgtagcc gggacgcacg ttcgacatat cttcaggcta
     3901 atgcgctcgc tttgcccttc gccgataaca gcttcgatca tgtgctttca gttgctgccc
     3961 tgtgtttcac taccgactgg cacaaagcgg tgtctgagat tgtgcgcgtg acgcgcaggc
     4021 gcttcgccat tggcatgctc aaccggaaca gcctgctttg gcgcgaaaaa ggtcgccacg
     4081 gcggcagcgg tgcctatcga ggtgcgctct ggttatcgcc tgaagagttt ggtgacagcc
     4141 tcaagggact acccatttca aacagccgaa tgcggagcgc aatctattta ccatcggggt
     4201 ctatcactgc gcgcaccgta gaacggctgc tccctggcag cgtaccgtgg ggcggattca
     4261 tggttctgac tggggacaag aaaccggtcg gaggttttgc ctcattgcca gtgtccaatt
     4321 gagctatacc ctatcctgat gtcaggtgct atgtgcaaac atcgtcagaa tagagtcata
     4381 gtttgtattt attgacataa accgccaagg gtaatagatt tcatcctgac acttttacct
     4441 ttggaggcat cttgcaaggt caacgcatcg gctatatccg cgtcagcagc ttcgaccaga
     4501 accctgatcg gcaactggag caaatcgacg ttggcaaggt attcaccgat aagggggttc
     4561 taagccagaa ccgccgaaat ttccgtcatc ccagctccag ccccgccacg agcgcatcca
     4621 ggtcgatcat cttgccgtcg ctctggaagg tgtaatgccc gttcagcagg atgtgccgcc
     4681 aggccgccgg cgatatctgg gtcaggagag cgtgcgcctt ggcgttgccg ctcgcctcgt
     4741 acttcatcag cagccgcgag aggatggccg agttgtagaa gatgaccgcg ttggcgatca
     4801 gcctggcgca ctggttgctg atctcaattt cgatgtcggt gcgcccggtc aattccttct
     4861 tgccgccgac ctgggcgatg gttgagcgta gctggtgata ggactcgatg cggttctgtg
     4921 agcggtgaac gttgcgctcc agttgcggat cgcgcaggta gcgcagcgtg tagatgctgc
     4981 ggatgagctt gtcgaactcg aacaccgcgc gccgcgtggg gttcggcgcg gtgtaggtgc
     5041 atagcttgcg gatcagcgtg ccctgcgtca tctccttcag tccgagcgtg gcgacaatct
     5101 ggtcgaggtt cggcttctcg ctgactatga gatcgcggtc gattctcccg gccggccgga
     5161 tcaggcactg atcgtacagc gccggatcgt cggcactgta gagttccttc aactgatcgc
     5221 caaggtcggt gaagcgcggc tcgaaacgca ggccgaacca gtgcaggata gcgaagttgg
     5281 ccttgttgac gctgtgcatg tcgccggtga tcgcggtcgg cacgatgtcc gacgtgttgc
     5341 gataccagat gtcgaacacg tgatgggcct cgtaatcgtg cgcgccgatc aggtagccgt
     5401 tgagcggcac gtggttgcac agcagcgtgt aggcgaccac gcccttgccg cgcccaaagt
     5461 atttgcgcga gtggcgcgct ttcacggtcg gccgctcgac gccgaatttc tgaccatcga
     5521 cggcaccgta cagtgcatcg aggtcgaacg agtagtacgg gaagatcggc agcgcggcga
     5581 tggcgttgct gatgcagtcg ttggccgcgt gcagcgttgc gtggcgcagg tactgttggt
     5641 aggagctctc cagcacgtgg tacgggatgt cgctggtgcg tgccatgacc tggttgccgt
     5701 ggttcatcgc ctgcgcgatg atgaccgcca tcaggctgtc ggcgtcggcg accttcttcg
     5761 catagcgcgg ctgcaaaggc gtcagcgccg acaggaactg gcactggccg ttgacgaagc
     5821 ggaacacgtc ggccacgtcg cagaacggca gttgctcgta gaacgccttc tcgcgcgcct
     5881 tctggttctc gcccttgggc ttgcgccatg tcagcttctg cgtgtccttg tcgtattcta
     5941 ggtgcgtcag cttgccctgc ttcagctcgc ggttgaaggc cagccactga gcgcgcagct
     6001 cggtcgcgag cgcatcgagc tgggcatcga gtggctgccg caggaacggg atgtcgatct
     6061 gcgccagcac ggcggccttc tcatccagcg aaaccagctc gtcggaaaaa tgccggtgct
     6121 gcaagctgtc gtcgaggtag agttcacccg actggaagcg cttcctgacc tggcggtaca
     6181 gccagaactc gtagcggtcg gcatgcaggt ccgtcggctt gccatcggca tcgaaggtca
     6241 gcaggtacgg tcgcaagcgt ttcggcagcg tggccgctgg acattcggcg agcggccgtt
     6301 gcgataggcg ctgctgtttg gcgaacacgt ccttggccca ggccagcgcc acgagccacg
     6361 ggctgcccgg atcagtgcca gcgaggtcga gcgcgacata cagcggccga agatggcggc
     6421 ggatgcgctc agccaggccg tccaccgcct gccagtgcaa agccagcttg ctcaccggct
     6481 tcacgctcat gcgctgcgcg gtggtttgca gcgtatcgcg gggcatgatt ttgtaggcgc
     6541 gctggcgcac atcgccgaac ggcgtgggat cgggcacgct gtcgtcgatg taaagcgaca
     6601 gcaggcggcc gacctgcggt gtgtcttgct gacggcgcac ctgctcggcg acaaaggatt
     6661 gctttgcgcc cgcactgctt tcgtcctcca actgcttcat gtggtaggcc atcgcatcga
     6721 ccaggttgtc ggaaagctgc cggtagcgca cccaggcata gcaaagcagg tagaggtagg
     6781 tctgatcggc cttcaggttg cgtagatcgt ggacggtgta gaagttcgcc aggctggcgt
     6841 agtacagcag attctgctgc gagacgccga gcttgggcag cagcgccttg gcgatccggt
     6901 gcagcggctc cagcgtggcg cgcttttcgc gttcgcgggc catctgacgc cagccaaagt
     6961 ccttggcgtc ctgcttgagc gccgccaatt gcgacagggt gtcgtcacgc actagaagcc
     7021 gacccagcgc ggccttggcc gattcgtcca acacttccga cagcaggcca gccagccgcc
     7081 gacgctcggc ggacagggct tcgctcacca gctcttgcag ggtggtatag ccgggccgga
     7141 tgatcttgtg ctcgtttagc cagacgatca gctcggcggc gatgaacccc ggcatcacgt
     7201 cgcgccgcac ggtctgcgcg gcctgctgcg cgagctgcgc caggaactcg gccgcccacg
     7261 gccggtagcc gaacagatcg gcaatccact cgcgctgggt gtagtgctcg tgcttggaga
     7321 ttggcttgtg ctcgaaggac tcgccgtgga agtagcggct cagcacgaag gcgcaatcgt
     7381 gctcgacctc actccagtcg aagcggaaga aggcatgctt ggccttgaag taaccgatct
     7441 gcaagatgca atagacctgt gcatggagac caggccggct gctggcgagc gccagttcgg
     7501 tttcagtcaa cgccaagtat tccagccgct gggcgtcgtc gaagtccggc aggccgtaca
     7561 gggcttcctg ctcggcgtcc gacaggacgg tcaatcgttc gctcttggtc gtcatcgcga
     7621 tgacgctcca cgagagcccg tccaaccaac ccgtgccagg gtctcgatca gcgtggttcg
     7681 cttgacgccg aagttgcggc acaccgccgc cttggacatg ccgccatcga gcgcagcgac
     7741 gatggcctcc agcttctcgc cggtgatcgc ctgcggccgg ccgccgatcc ggccgcgttt
     7801 gcgggcggca gccagaccgg cgacgacacg ttcctggatc aaggcgcgtt cgtactgcgc
     7861 gagcgcgccg aacacctgga acagaaactc gcccgagggc gtcgtggtat ccaggttctc
     7921 cgtcagcgag cggaacgcca cctgcttttc cttgagcgag gtcacgatgg cgagcaagtg
     7981 cgacaacgaa cggccgagcc ggtcgagctt ccacacgacc aacacgtcgc cagggcgaac
     8041 gaattcgagc gcccgcgcca ggcccgcgcg gtcgtccttc gcgccggaag catgatcctc
     8101 gaacagatgc cgcgcatcga cgccgacggc gagcagcgca tcgcgctgca agttcgtgct
     8161 ctggcggtcg gagtccgacg acacgcgcat gtaacctacc aacataagcg gaaaaccatt
     8221 aagacgaggt ttccgtatgg tagcgtctgg cgacagagtt ttccgcacaa ttttgcggcc
     8281 actcggaggt tggtgcagag gatcgttgaa ggcctgtcga aaaacaaatg ttttccgaca
     8341 ggccttggcc tagcgcacgc ccgccttgca gcgttctgtg aatagcgctt agtaatgacg
     8401 gcgtatatac tttgttagta tcaagtggca ggaggccccg atcatgtcaa aacaagccgt
     8461 tttcacgatg aagctggagc ctgagttgcg cgccgagttt atggccgaag ccgaggcggc
     8521 ccatcgcccg gcgtcgcaag tgctgcgcga gctgatgcgc gagttcgttc agcgccagcg
     8581 cgagtcgcgc gagtacgacg agttcctgcg ccgcaaggtc gaagccggcc gggcttcgat
     8641 gcgcgctgga ttggggcggt cgaacgatga agttgaggcc gaattcgccg cacggcgtgc
     8701 cagtgtggcg agccaggcgt gagggttgtt tggacgcccg aagcgcagca agaccgtgcc
     8761 gatgtgtggg actacatcgc agccgacaat ccgcgcgcgg cggcccggat ggatgagatt
     8821 ttcagcgacg cggccgcccg cttgatccag caccccatgc tgggcaagcc gggaaagatt
     8881 cccgggaccc gcgagttgat cccgcacgaa agctatcgcc tggtgtatca gatcgacggc
     8941 gaaacggtgt ggatactgac gctggttcat actgcccgcc tgtggccgcc tgtgcgcgat
     9001 taagagatga tcatggcaac aatgaaacgg cgcgttgaca gactgctttc gagccagacc
     9061 gcggtcggcg gcaggcggcc agttgcggac ggtcaagcat cttcccggta gcggacgttc
     9121 aatagtgtct ctgacctgct tcgctccctc tatgaaggga ggtcgctggc ccatccgcgc
     9181 actcacagcc tgccagtagg cgcaaattgg cagcgctccg tccggcagcg cacagactgc
     9241 ggccgtacgt ccgcgtaggt tcgcttcagc agtttcgatt cgaaagttca atgtgattgc
     9301 tgcgggcggt cgtcgcagtt gcgcggaggc gatgatgcac aatcacggga tcggcagata
     9361 ggttcttcga agctgcaaga gttaaaacat tcacacatga aagattgaaa tggatacacg
     9421 attatttgct ttcgtcggcg cagacattgg cccttggcgg attgtcaggg ccgaaacgag
     9481 agttggcgag cccctgcctg aagccaaaag gctcaacgtc gtatccgctt cagagttgca
     9541 gtctgaaacc aatgcaccct ggatacttcg tggaataacc agcaatgaac gatatgtcat
     9601 gcgcgcagag aagaatgaaa ttgtagcgaa gcagcaaggt ctggcgcgcc cagaagcaac
     9661 gtgcggtgcg ctaataccga tccggaagaa cgcagcttgg tgggagctca cgcaagacga
     9721 acgccggagc gttttcgaac agtcgaagca cgtccaaatc gggcttcagt atttgcctgc
     9781 agtagcgcgc aagctccacc actgccgcga cctctcagag aatgaaccgt tcgattttct
     9841 gaattggttc gaatacgcgc caattcatga ggttgagttc aacaggctgc tttccgaact
     9901 gcgtgcgtca gaggaatgga aatacgtcga ccgagaagtc gatattcgtc ttacgcaagc
     9961 acaggtctaa cccgccgatg aacacggacc cccaacagcg gcgcgttgcg ccgccgtttc
    10021 gggccggtta tcggcaacgt agaactctcg gatacagttg ttctgtaata acttctcaag
    10081 ccaaattgat ttttggagtg gatatggaaa tggaatcaag aatattttcg gtaacagaat
    10141 atattcgtcc gtctgacggc gaaccaattc gttcagtggt tctggaaacc aaggactcag
    10201 cagttgttgt ttggcatgcc catcctgggc aagaaataac cgctcatgtt cacccggacg
    10261 gccaagatac ttggacggtt atctctggag aggctgagta ttaccaagga ggcggcaaag
    10321 tcgctcatct aaaggctgga gatattgcca tagcaaaacc tggccaagtg catggcgctc
    10381 taaatactag tccagtgccg tttgtatttg tctcagtggt tgcatctggc aacgcgggtt
    10441 ttgcgttggc tgaaaaatag tctttctcaa atgtgtgccc cagagagttc taacccatcc
    10501 atcaacacgg acgctgcgcg atgaagccgc gccgcgccgg ctaactccac gttgagcgtc
    10561 agctttccat atgtctgggg gtctgcaacg ggtcgggtgc cgtcgcgtgc gcagtgtcaa
    10621 agctgacatg actaatacat gagatgggct gatgctcgcc gtccggtcag gcagctgccc
    10681 ccctgcagga ctcacagcag ttgtatgcca cccgtcatcc gagacgtcag tcggcctcat
    10741 ccagaccgga acaatctgga cgtgacgtcc ggctggcatt gccgtgatcg aaatggcggc
    10801 tagtgacacg actgttgggt ctaccccatc gaccgggcgc gggacaagcc acgttgatcg
    10861 gctgacggaa ttttcggcgg ttccggctta gaaccccata aggcttccgg caaggatacg
    10921 caacgtcccg agcttgaaag gctgctggcc ttcgtccgcg agggcgatac cgtggtggtg
    10981 catagcatgg acaggctggc acgcaatctc gatgacctgc gccgcatcgt tcaggggctg
    11041 acacaacggg gcgtgcgcat cgagttcgtc aaagaagggc tgacgttcac cggcgaggac
    11101 tcaccgatgg ccaatctgat gctgtcggtc atgggggcct ttgctgagtt cgagcgcgcc
    11161 ctgatccgtg aacgtcaacg cgagggaatt gtgcttgcca agcagcgcgg tgcctatcgg
    11221 ggacgaaaga aatcgctgaa cagcgaacaa attgccgagt tgaaacggcg agtcgcggca
    11281 ggcgatcaaa aaaccatggt ggcccgtgac ttcggcatca gccgtgaaac cttgtagagg
    11341 gtcggcaggg attcatgtaa aacccagcag aaacaggctt aagtgcctgt ccccgttgac
    11401 tttttcgact ctctcttccc tggacgaccg gcctcttcaa ccgggttcgc aaccaaatta
    11461 gggccagtcg ccggcgtact gaagcgctca agcatgctgg ccacgacctg ctcctgggcc
    11521 gtgacggatg acctggccgt cgccagcgcc agttccagct gttgtttggc ggaaagcagc
    11581 tcgtcgacct tggcctgcaa ctgctctttg gaagtgctga gctgctggac ggccgcgtcc
    11641 tgctcaacca ggcgccggcc caattcctcg gagcgccttt gcgcaaaccc cagttcatcc
    11701 ttgagcggcc gcaggcctcg cacctcttcc tgagcctggt gcagatcgcc ctgggcgcgc
    11761 gacaaatcgc ccaggaggcg tgcgttttcc tgaagggtat ggacggcttc ctgctgcttg
    11821 gtggccaagg tctcgttcac ggtgcgcaac tcggcctgca ggtactgcac ctgctgttcg
    11881 tgcttgcgct ggtcctggtc gcgctgctcc tttgtggatt cccggaaatg ctccagcgcc
    11941 tggcgcgcgt gcttgtgctt ttcttccagg gattggcgat ggcgctcttc ggccgcgagg
    12001 cgctcctgca ggtcggccac ctgctggacg agctgagtgt tttccagggt cttgcggctc
    12061 agagcttcgc tggttttgcc gtgagcgttt ttttcgtcgg ccagggcccg ctgggtttgc
    12121 tccagctgct ggcgcgtggc cagcgcctcg gatttcaggg ccgcgacggc ttgctgctgt
    12181 tggctcagct gctcggcgtg cttggcctgc gccgtggtga cccgctcttc cgcttcttca
    12241 ttgacgcggg cggctaggcg acccaccagg tcctggattg cctcgctgac ggctacgcgg
    12301 ctgccggtgg ctggcccttc ctcttcctcg atctccttca ggtagcgatg gatcgtggtt
    12361 ttggagccgc tgcccagctc ttcgcgcacc gcatcgatgg acggattgcg ccctgcggcc
    12421 agcaatttat cgcgggcacg cagcacttca gacttgtaga ttccggctcg ggccatggtt
    12481 cacctcgtta tttcgtactg tagtatgtac catgatatta catactattt aagaggtcaa
    12541 taaatcatag atcttagtaa atatcacact gggtaatggc agattatcca atgtgatgct
    12601 ggtttggggg tcgatttaga tgtggcgcgg gtaaggcgta acgcgatcag acctgctgct
    12661 gcgccgcgga aactcacccc gtcgcgttcg cgcacaacaa cgctgtaacc gcacgcatgg
    12721 gggcacggct gctagcagcc tgctgaaata ccaagggctg aacccgggtt ggcgtcactg
    12781 gaatcgtgat gtcggtgcct tggtcgttcg tgagggtctg aatacgcgtt tggagctcga
    12841 ttcgacccgc gcaagcgccg gttttcggct ttatgcgctc atcgcgcacc ctgcagacgg
    12901 acctgtccca gtgaacgcat gcgcacgagg ttgtaggcgg ccatgttcag cacgaacagc
    12961 tgagcgactt tcttggtccc acgcaccatc acttgacgca cctgaccgat cgtcttggcc
    13021 catccgaagc cttgctcgat gcgtttgcga cattgcatcg acgcgccata gcctgcggtc
    13081 tgggcaatct catcgggcac cgccgagcgc cgccctgatt tgttctgcgc cacgtgcggt
    13141 gcaaccttga gtcggtgcaa ctcaccgaca aactccgccg cgtcgtaccc cttgtctgcg
    13201 cccagggtga tggccgcttc tgggttggcc tgccgcacgt cgttgatcat ctccttggcc
    13261 gctacgcgct cggcataacc atcggcctgc gtgaccatgg cattgacgat caagccgttg
    13321 cggttctcca tgagcgtgtg ccccatgaag cgcatttcgc tggcggtctt gcccttgcgg
    13381 tacagccggc tgtccggatc gctcttggat tcgtgggtgt cgttgctgcg cgggcggccg
    13441 cggaagtcgc tgccaccgct accacctcca tcgccgtcct tgggcacaaa gctcttgtgg
    13501 ctggcccagg cctggatcag cgtgccgtcc acgctgaagt gttctttgga caaccagttc
    13561 cggcgctggg cctgcgcgag cacctggttg aagaacgcaa ccaccgcgtc gtgctcaatc
    13621 agccgctggc ggtttttgct gaacaccgta ggcacccaga cgctgtcttc catcgacagc
    13681 ccaatgaacc agcgaaacaa caggttgtat tgcacctgct ccatcagcaa gcgctccgag
    13741 cggaccgagt acagaacctg cagcagcatc gcgcgcagca gcttctgcgg ggcgatgctg
    13801 ggacgcccgc ccttgccggc gtcttcatac atgcctgcaa acagatcttc gagctcgacg
    13861 agtgcgtcgt tgaccatcgc gcgaatggct cgcagcgggt gcatcgccgg cacgaagtca
    13921 tcgagtttct tgatgctgaa caggctttcg gtgaaggtgt cggatccgcg catgccgcat
    13981 tgtcctgcct acgccttcgt ctggatcaag tcacggggct tgcgtcgaag tatttcagca
    14041 gcctgctagg tgggaaagta cgaagccatt tcctcattgc ccaccgagga atcggccaat
    14101 ccggcgcatc tcaagcggac cctggcctat cagaagattt cgtcctcgcc accgtccccg
    14161 tcttgccgga cgcaaccgga cgaatgccat gggccagcag cccgatgaaa atgtcggcaa
    14221 cctcatcgcc acgcaggcga ccttctgcgt ggaaccactg ggaaatcccg ccgacggccc
    14281 ccatgaacca gaacaccgcc agcttggcat cgcacggcgc gatggacccg tctcgaacgc
    14341 catcctcgaa cacctggcgc aactccagat cgaacttgcg gcgcctcgcg gtgatcttct
    14401 tctgatccgc cgccgtcatc gccgagaagt cgtggaggat ggcgggggcc cccatttcgt
    14461 cggtcagcag tgagatgtag ttcttgatga aggtcgcgat gcgttcatac cccgttgttt
    14521 ccgaggcaat ggcatctttc aggacctggt ctccgagcct catcgacagc tcgtggcact
    14581 cgtagatcag ttcctgcttg ttcttgaagt aatagtagag cgcaggcttc gtgacgttga
    14641 gcgcggcggc aatttcgtcc agggacacgt tcttgtaccc gtggcggccg aacgtgcgag
    14701 acgcctccgc aatgaccgcc tgcttcttga gttggtattg ctcgtccgcg ccgccgaccg
    14761 cgttgttcca cttcactccc ttggccaggc gtttggccgg gccacggggt cgcggcttcg
    14821 gcttggcgtc gatttgcgtg ctcattcttg cagtgtcctg tgcgcgtttg cccggagaag
    14881 ggctgagttt cgatgatatc ggacaggcaa aatcacagag ctaagcgtcc gagcgtgcgt
    14941 caacccgcca gtatctgccg ggcgatgatc agccgttgaa tgtggctggt cccctcgtag
    15001 atcttcgtca cccgcacgtc gcggtagtag cgctcgacag ggaaatcgtt cacgtaccca
    15061 tagccaccgt gcacctgaag cgcagcggaa cagaccttct ccgccatttc actggcgaac
    15121 aacttggcca tcgcggcctc tttgatggac ggaagcttcg catccgtcaa acgcgccgca
    15181 tacaacatga actggcgcgc cacgtcaatc tgcgtggcca tgtccgccag gtcaaagctg
    15241 atggcctgta attcggcgat cggttttccg taggcctcac gctccttcgc gtaccggatc
    15301 gcggcttcca gggcagcgcg ggcaatgcca atggacagcg ctgcgatcgc gatacggccg
    15361 tcggacagca acgagagggc cttgccataa ccgcgccctt cctcgccaat catgttcttc
    15421 gccggcacac gcaagttgtc cagctgaatc tgggcggtgt gcgccgtgtg ctggcccagc
    15481 ttgttttcga ttcgaaggca ttcgtagccc gggtccttcg ggtccgcgat gaacaagctg
    15541 aagcggtcgc gagcgcccgg tgctcccgtg gcggcgacca ccagggcaaa gccggcctcg
    15601 ctgccgttgg agatccactg cttgcttcca ttgagcacat agctgtcacc atcgcgcttg
    15661 gccgtggtgc ggatggctgc agtgtcggaa ccggcatgcg gctccgacaa cagaaaagcc
    15721 ccaatgcgtt gggcgctggc caactcgggc aggtacaact gcatctgctc ctcggtgcca
    15781 aacgatcgga gcacgtgcgc ggacgagttg tgcacatgca tcagggtcgc aaaaccaccg
    15841 tccgccgcgg cgaactcctc gatcgccaga cagtactcca cgaacgacag cccggcgccg
    15901 ccataggcct gtggcgccag catccccatg aagccaacct cggccacagc gcggatttca
    15961 tcgtagggcc aggcgctgct gcggtcacgt tccgccgcag tgctggccac cacttcgctg
    16021 gccacgcgac gggccgactc ccggatcatc acttcctgtt cgctcaaaaa tgcattggaa
    16081 acgctcgtca tcgctcatcc ttgaatgttg tcgatcctgc ggggcacgtg cccatcagcc
    16141 cagccaccgc tttcggcggc ggtaatgttt catgtccctg aagctcttgc gctcaccgtc
    16201 caccgtcaga cccaaataga actcgcgcac gtcctcgttg ctcttgaggt tgaccgatgg
    16261 gccttcgagc acgacccggc cgctttccat cacgtagccg tagtcggcca gctccagcgc
    16321 gacgcgcgtg ttctgctcga cgatcagcag tgtcaggcca ctggccttga gttcagacag
    16381 caagccatag acctcttcca ccataagcgg cgccaagccg agcgagggtt catcgatcat
    16441 gaccagcttc ggtctggcca tgagagcacg cccgatcacc agcaactgct gctcaccccc
    16501 ggacagatag cccgcggtac ggttgcgcaa tgcggcgagc cgcttgatac ggccgtacac
    16561 ctcgtcaagc agttccttgg catcgcgccc attgcccatg tgcccgccga ccaccaggtt
    16621 ttgttccacc gtcatgtggc gcagaacctt gcgaccttcc atgacgtgta ccagccccct
    16681 ctggacctgg gcggccggat ttccccccgc gatgtcgaca ccatcaaagg tgatggcgcc
    16741 tgccgtcacg cgaccgtctt cggtcttgat caggttcgaa atggccttca gcgtggtgct
    16801 ctttccagca ccgttcgcgc ccagcaaggt cacgcactgc ccagcaggca ctttggctga
    16861 aatgcctttt acagcgagaa tgacatcgct gtacaacacc tcaacgttgt tcagttcaag
    16921 cacaagtgtc tccggttggg ttggcgtgga tcccgaacga accgtatcca cgccgggttg
    16981 cagttaccac ttgctcagca caggcacggg gacgctctgc tcaaggagct tgtaatggcc
    17041 cttttcaaca gtaccgatgt tgactgccat gccttcggtg gggaacggcg cgtctttgct
    17101 atattggata ttgggcagca caccgaagct gcgttcgctc ggcatcggcg tcgacagcag
    17161 cgccgcccgg acgtcttccc ccttgacgtc cttgacaccc ttgatcttga ccgcctgctc
    17221 gaaggctctg gccacgataa ggccttggct catgcccatg atgcaggctg aggtgtagct
    17281 gacctgtgcc ttgtacttgg tgcgcagctt ctcgaagaat gcccgcgccg tggtcggatc
    17341 ttccgtgggc atggccatcc cgtagacctc gtagttgcct tccatctgct ctagaccgcc
    17401 caaggccttg cccgacgaaa tcaacccatt gtgggccgac atgacgatcg ggatgttggt
    17461 ctttccaagg ctttgcaggg ctcgacgagt cgccacgacg gaggccgtgt tgttgaagac
    17521 ttgaagcacg gtcgcgccct tgcgaatcaa ccggttcact tgctgggtca ggtcggtggg
    17581 ttgggcttcg gtgtagaccg tctccacgac ctccaagatc ttggggttgt ctttcgcgaa
    17641 cttctcgatg cccttgtgga tatccacata agccggggac acttcggaag agatcacacc
    17701 gaccttgatg ggtgcgctgg agcccgattt cttctgcaac caggtgtaga acgccgccgc
    17761 ctcgtgcgga taggtggccc gggcgttgaa cacccagctg tcgctcttcc acgcgaagcc
    17821 ataaccggcg gtcgcaagga cctgaggcac cttgtcattg ggtaatcgct gctgcagggc
    17881 cgccgcatcc gagccgccga tacccagaat ggccacgggg tccagttctg acttgatgcc
    17941 gggccacaga ctggccacct gcgccacgtc gtagcgcgtg tcgtattcct tgacgcgaag
    18001 tgccacgcct tgcgccttgc ccacctcatc gctccaccag tcgagaacgc cttttcggca
    18061 acccgtcatg tctttcatga catcggcaaa cggaccggta aagtccgcca agccagcgac
    18121 gttgtaggtg gtctgtgctt gggcggctcc cgccaaacca aacacaccgg cggccaaagc
    18181 accgcatcgc gccacacggg cgaaggccga cagacattca ttttttgtag tcacacaagt
    18241 ctcctggttt tgatcacgct gtccaacacg aatcaaccgt cctccagcgc agcggacggc
    18301 cgcaagtttc ggcatcagta ggggtaaggc cacatgcggt aggaacgttt aaggatgttc
    18361 caccggtgca tcaatccacg cggctcaaaa atcagaaagc cggcgatgat gccgcccaga
    18421 aatatgttca gcgcggcgaa gaccacgtcg ccactcagga acgagaagtt ctcaaccagg
    18481 gaggggccca cgctggtgaa gaattcctga gcaaaccgga tgacgaatac gccaatcagc
    18541 gcgccggtca ccgatcccat gccgcccaca atgaccatgg cgatgaacca gatagagtgg
    18601 atcagtgtga actggtcgat cgagacgaag cgcacgtagt acgcccacaa accgccgccg
    18661 atgcccgcgt agaaggcgcc cagcaggaaa gccaatgcct tcgtccgcac tacgtcgatg
    18721 cccatcattc cggaggcgac gtcgtcatcc cgaacagcgg caaagatccg gccatggcgg
    18781 ctgcgcgcca cgccataggc gcccgcgatc atgatgcagg cgaccaccag acacaggtag
    18841 tagacggctg catcggtgtc gagcgcccag ccgaacagcc gcgccggttc gaccgtgatg
    18901 ccgttggagc cacccatcca ggcagcgggc aagttcagca ccatgaaatg gaacaggatt
    18961 tgtgccgcga tcgtcgtcaa cgccagatag aaacccttga tccggacggc agccagtcca
    19021 aaaatcagac cgaacaaggc ggcgcacaca ccaccgactg gcagcgcgat ccagaacggc
    19081 ataccgccct tcgtcgctag cagcgcggtg gcgtacgcgc ccgcgcccat gaacgccgct
    19141 tggcccaggt tgatctgacc ggcaagaccg gtgttgatct gcaacccgat caccaccacg
    19201 gcggtgatca gcatcgacgt catcaccgcc accgaacggg cgtcggcgat ccacggcaga
    19261 gcgaacatga acataacgaa caagcccagc gtcacggcct gacttcgcgt gcggaccagc
    19321 gcctggtctt ggtcaaacga cgttgcgaaa acacctgcgg gatccatgct catacccttt
    19381 caatggtttt ccagccgaac atgccggtgg gacggatcaa caacatcaaa agcatgatga
    19441 tgaaaggaaa gacagagccc agtgccccgc ccaccaacgg atcaaggtag gccgacgcca
    19501 gcccctggat gacaccgacc agaacgccgg ccagcagcag tccggcaagc gattccaagc
    19561 cggcgagcaa ggccactggc agcgccgcca gaccgatgtc ggaactcatc atgttgatcg
    19621 accgaccgct caggtgcacc atggcgccca gcgtcgagag aacggctccc agcatccagg
    19681 cgaatgcgat tgagcgctgc accgaaattc ccattgacat cgccacctgg tgatcttcgg
    19741 ccacggcggt catcgtcaag cccacgcggg tccggttgaa gaaccaagaa aggccaaggc
    19801 caatgacgat ggtcaggatg ccgccataca gaagcgagcg ggagatcacc acatcgccga
    19861 ggatgatggg ttccagcgga aagatgatcg ggaacgcgcg gacctgcgga ccaaagagca
    19921 ccaggacgat gcctcgaatc aagatcaaca ggccgatcgt caccatcacc atcgagaaca
    19981 ccgactcgcc cacgagtcgc gcgaaaaata tgcgctcgat catcaagccg aagatcgccg
    20041 cgctggcgaa ggccaccggc aacccgatcc acaccggcat gcccatctga atgatggtcc
    20101 accacaccat gtagccacca aagaccacca gctcgccctg tgcaaagttg aacaccttgg
    20161 atgcgcggta gatcaccacg aagcccatcg cgataagggc atagagaaga ccggacaacg
    20221 cgccgttgac caggtagttc agaaaatcgc tcatgcgtgc ctcctgcgat ggggttcggt
    20281 cgaagtgttc aaactgaatc cgtcagcatc gtgaatgaat acctgcgcgt tgaagttgct
    20341 tcgacgcccg tcctggtaag tcacaggtat gtcgatatgt acttcccggg aaccgtcgta
    20401 caagccctgg atcaaggcac cgtaacgctc ctccaagaac tcgcgccgca gcttgcgcga
    20461 acgcgtcagc tcgccttcgt cgggatccag ttccttggga aagttggcga accgctgcac
    20521 ccgtgcatgg ggtggcaaaa accggttgac ccgcgcaatt tcctggcgga tcaagaccgc
    20581 gatctcgggg cgctgcgaaa ggtcggtgaa ggtcgagaaa ccaattcgct tgtcctcggc
    20641 ccagcgcgac aggactgcca tgtcgatgtt gatgagtgca gccacgaagt cgcgcccctc
    20701 atctcccacg gtcatgatgt ccttgatgaa agggctgaac cgcaaacgcg tctcgacgaa
    20761 ctggggcgga aaggtctcgc cactgcgaag tcgcttcaaa tccgacagcc gttctaggaa
    20821 aaccagctca ccgcggtcgg tcttggtcac ggcgtcaccg gtgtagaacc agccgtcctt
    20881 gaccttggac tcgaccgacc cttcccgtcg gtagtagccc agaaaagggc ttccaccacg
    20941 gatctggagt tcgccgtccg agctcacccg ccattcaagg ggagcaccgg cctctgggtg
    21001 cgcctgcatc cagtgcccta cggtctcaag gtcgaagcga tcactctggt ggcatgtgag
    21061 caagccggtt tcagtggagc catagatgtt ccgcaaggac acgcccatgg catggaacat
    21121 acgaaacacg tcgggtgcca tggtggcacc accgcaactg gcaatgcgca gccgggtcag
    21181 ccccaactgg tcacgcaacg gccgcagaac cagagcttcg gccaagggga attgcaggcg
    21241 atccagccag ccgatcggct tgccatccag gcgaccgacg ttgaaggcgt gccccacctt
    21301 cataccccat tcgtagacgg cacgccgcag gggcccagcg tccagcatgt gggcctgcac
    21361 cgaggaggcc atgctttccc attgacgcgg agcgaaggtc atggcctcga cggcgagctc
    21421 gcggatgttg gccagcacct gctcggggct ttccgggaag ttgaccacca tgggcagccc
    21481 caggcccgca gagatcccgt tgatttgctc ggtggcccag gcaggcgcga tgtagctcag
    21541 atactccatg cccggtttcg catcggtggc atggatcagg cgctgcgcgt tgtcgatcag
    21601 gaagcggtgc gtgagcacca cccccttggg tttgcccgtg gtccccgagg tgtaggacag
    21661 caccgccaca tcatcgggcg aacccttcgc gaccaacgaa cggtacagcc gagggtcctg
    21721 cgcatcccgc tcaagccctt gctgctgcac cgacttgaag gtgctgagga tcgggtcgtt
    21781 gtaggaccac atgcctgtgt catcccagta cacgacatgg cgaagcagcg ccagcttgcc
    21841 cttgacgtcc aggcccttgt ccacctgttc ctggtcctgg gcgaagaggc agaccgtctc
    21901 gctgtcctcc agcaaatact gcacttcgtc cgccgtcaga tccggataca gggagaccac
    21961 tttgcagccc agcgcgtgcg ccgcgaactc cgcccagtag tgctgcggct cgttctcgcc
    22021 aatgatcgcc accgtcgcgc cgcgctgcac gcccatgtgg tgcagaccca gtgccagctc
    22081 cagcacattg cgctccacct ccgcaaaggt gtattcacgc cagatgccgc gctgcttgtg
    22141 gcgctgagac agccgctcgg gtgtggcgtc cgcgtggcgt cgcaacagtt gcggcagtgt
    22201 ttcgatcttg agcatcattg cccgccctcc ccgagatagg ccttcatgac ggccgggttg
    22261 gcctggatct ccttgggggt tccctctgcg atctttcgac cgaagtccag caccgcaatg
    22321 cggtctgcca gatccatcac cacgcccatg tcgtgctcca ccagcacgac cggaatccgt
    22381 ttggcttcgc gcacgtccag aatgaagcgc gcgaggtctt ccttttcttc cgcgttcatg
    22441 ccggccatgg gctcgtccat caacaggatc cgggggtcct gggccagcgc ccgacccaag
    22501 tccacgcgtt tgcgaagtcc gtagccgagg ctgcccactg gtgcatggcg gatggcctcg
    22561 atctccagga actcgatgat ttcctccacc acttcccgat ggaccacctc ttctcgccta
    22621 gcggcgccga agtagaggaa cgactgcgcc aggttcgagc gcatgtgcat gtggcggccc
    22681 accatcaggt tctcgaccac cgtcatgccg gagaaaatgt gcgtgccctg aaaggtcctg
    22741 gccaagcctt ggcccgcgac ttcgtggacc gccatgttgc gaacgctcag cccgcctagc
    22801 gtgatgtcgc ccttcttggg acggtaaaaa ccgctcaggc aattcatgag cgaggacttc
    22861 cctgcgccat tcggaccgat gatggccagc agctcatcct tgcgcacatc gatggacaca
    22921 tcgttaagcg cgacgattcc accgaactgc agcaacaggt tgcgagccga gagcaccaca
    22981 ccgtccgggc tgacactggt ggttgcaatc atgacgcgtg ccccttgacg ggtgggatca
    23041 tgtccatcga ttctccttgt tcggaccgtt gtagggtctc gtgatggtgt tctgttcggg
    23101 ctgccatgtc cgataccgtc agagccgtgc cccgccatcg atgctcagcg tggtggcgtt
    23161 cagataggcg ttctccacca catgacggac cagcgccgag aactctggcg cctgccccat
    23221 gcggttcggg aacaggatga ctttctcgat aatgttttgc gccaccttag tcggcatgcc
    23281 tgccgacatg ccggtctcga acaagccggg ggcgagcgcg acgactcgca cgccgtgctg
    23341 cgccaggtct cgcgccaccg gaagggtcaa ccccatgact gccgccttgc tggcgctgta
    23401 ggcggcctga cctatctgcc cctgccaggc tgcacctgaa gatgtgttga cgatcacgcc
    23461 acgctcgcca tccgcgttcg cgtcgttctg cgacatggct aaggcggcgt gccggatgac
    23521 gttgaaggtg ccggtcaagt tgacaccgat cacgcggttc cagaggtcca gcggaaacag
    23581 ctcgcccttg gacaaggtct tgcaagggcc gaggatgccc gcgcagttca ccgccacatg
    23641 taacgcccca aacgtgttga ccgcgaactc gacggcagct ttcacactgg cgtcgttggc
    23701 cacatccgtt ttcagcgcac gcacgctgtc acccaacggg accatctcgc gcatcaggcg
    23761 gtcatcatcc acgtcgaccg ccaccacctt ggcgcccgca tcgaccaggg ccttgcaggt
    23821 cgccaggccg agccccgaac ccgcaccagt caccaacgca accgtgttct tgatgtccat
    23881 gcttgtctct tctttcggtg aggcgctcag cgcgccagga tggagatgca ggcaacggct
    23941 tcctcgatgc cctcaaggcc gccgccgttt tcggccaatg cgatgcgggc gccttcgact
    24001 tgccgcgcgc cggcttccgc gcgaagctgg gtcaccagct cgtgcacctg cgccaagccc
    24061 gtcgcaccaa ccggatgccc cttcgactcc aggcctcccg aggggttgat gggaatgcgc
    24121 ccaccgatgc gggtgtcgcc ccgctcggcg atcacacccc cctggccgaa ctcgcagaag
    24181 cccaggtttt cagactggat gatttccccc atggcagtgg cgtcgtggac ttcggccacg
    24241 gagatgtcgt ccggccccac gccggccaac tcatacgccc gccgcgcggc cagcgccgtg
    24301 caatggttgg caacgtcttc ctcattgcgc gcggagccgc tttgaaccac agcggccagc
    24361 acccggattg cccggctcgc gtcgatgttc aaccgcttga gcgcagaccg gttgcacacg
    24421 atgaccgcgg cggcgccgtc cgacacaggc gagcacatcg gcaaggtcag cgggtaggtg
    24481 atcggtggcg ctgtcagcac gtcgtcaatg ctgtacgaca cttggtattg cgacagtggg
    24541 ttgtggaccg aatgcgcgtg gttcttggcc gagacggccg ccagctgccg ttgcgtagtg
    24601 ccgaaagtgc gcatgtggga gcgcgcgaag gccgcgtaca catccatgaa cacgctgtag
    24661 ggccggggag aggtcgagcc ttccggaacc gccacatcgc gcccgagctt catcagctct
    24721 tcgcggatgt cggtggcccg cgacagatcc caggcgctgt cgaagaccga gaacatcttc
    24781 tgcttgtccg gggtgaacat cttctccgcg ccgatagcca aggccacatc gcccgcaccc
    24841 gcacgaacat ggttgcatgc caacacgaac gcggacgatc cgctggcgca ggcgttttcg
    24901 atgttgacca cgggaatgcg accgatcccc atagcgcgca atgcaatctg cccgcggatc
    24961 atgtgctggc cctccatgtg accttgggag gtgttgccga aaaaggccgc ttgcaggtcc
    25021 tcttgccgga tcccggcatc caccagcgcc gccttggtcg cctcgcgcgt catctgcttg
    25081 atgtccttgt ccagatggcg accaaacggg gtcattccca caccaatgac atagatgtct
    25141 tccaatgcat ttctcctcta agcgggccta tgggaaagcg cctcgttgac gccaatctac
    25201 tatttataaa ggtgttttac tgtagagtaa aattgatgtc gaagcactag ggaaatcccg
    25261 acatccgatc agacgtcggc ggctcgatcg gtcagtgcaa ggaagcgcaa tcccctcgcc
    25321 gtctcgatat cgccacccgg cgtcacgcgg tacaagccac gcgccacgtt gtgggggtgt
    25381 gcggctgcct cggtcaggtt caaaaccggc gcatagcagg catccgtacg ttccagaatt
    25441 tgcgtccaat gggcactggg gtgcgatgca aaaagagcgg tcattcgcgc tttcagcgcc
    25501 ggccatgtag accggttcat ctgatcggac aacacgacat cggtcagacc caggcgctgc
    25561 aggagcagcg cgtagaactg gggctcaata gcccccacgg tgatgtagcg gccgtctgcg
    25621 cagatgtacc ggtcgtagaa gggcgagtcg tggaaaacac tgggcacacc cggatcaaaa
    25681 cctccagagg cgctgatgat ttgcaccagc ggggacaaca tcgccagcac gtccactatg
    25741 gcgccatcca ccacgcagcc ctgcccactc tccctggccg cgagcaaccc cgacaccatg
    25801 cccagcgcga aacccagcgc acccgccgca tcacccaaca cagtgggagg cagcatgggt
    25861 gggtggccag gacgctccgt cagcgacatc aggccggtaa gcgccacata gttcatgtcg
    25921 tgcccggcgg cctgcgccag cggcccgtcc tgaccccaac cggtcatgcg cccgtacacg
    25981 agacgcgggt tcagttgccc acagacatcc ggtccgaggc ccaggcgctc catgacgccc
    26041 ggacggttgc cttcgatcaa tccgtcactg gccgcgacga tctcaagcgc ccgggtgcgg
    26101 tcgtcttcct gtttcaggtt caggactact cggcgcttgc cgcgagacag caaaccatcc
    26161 tcgcgcggcg tcagatcctt cggcaagtcg ccggggctcg gacgcgccac catggtgacc
    26221 tccgccccca attgcgtgag cagcaagccg gccagcggcc ctggtccaat gccctcgaac
    26281 tcaacgacct tgacccgctt cagcggagat ccacgcgatg caatctgatg gctcatggtg
    26341 ctcttctggt tgattcattt ttactgtaaa gtaacttaga cgactttgta gtaatggaaa
    26401 tccccaacaa ccgatccctc ggttgaatcg cagaatctac tcatgaaaaa tcaagccatg
    26461 gaaagagaac aaatgccacc acccgctgtt gtcgtggatg ttcgaaaagg tgccatgtgg
    26521 atccgcctga atcggccgga ggcgatgaac tccctcacgc cggaggtgct cagcggcatc
    26581 gatcaggccc tgagcgacgc cgaggggaac caggacgtgc gggcggtcgt cctcaccggc
    26641 acaggtcggg ccttctgcgc cggggccgat ctcaagtacg tccgatcgca atcgggatcg
    26701 gacggccctc gtctgtttct cgacagcgtc ctggccacga tgaaccggct ggacagcttc
    26761 cccaagccgg tgatcgccgc gctcaatgga ctggccctag ccggtggcct cgagttggtg
    26821 ctctgctgcg acctggtgct tgccgctcgc ggcgcaaaga tcggcgatgc ccatgccaac
    26881 tacggcttgc tgcccggcgg aggtggctcg gtgcggctgc cccgcgtcat aggacccaca
    26941 cgcgcgaagt acctgctttt caccggtgag tttctcagcg ccgacgagct ggtggaagcc
    27001 gggttggtca accaggtggt cgatcccgat gaactggaag cggccacgca agcgcttgtg
    27061 gacaagatcg ccagcaagag cccattgggc ttgaggcgca tgaaggcgct ggtcgatgat
    27121 ggcctgcaac aaccagtcga gacggcgttg cgtctggaac tgctcgcgtc ggaggtgcac
    27181 tcgcacagcg ccgaccagcg cgaaggcttg ttggcattcg aagagaagcg cgcaccgcga
    27241 ttcatcggtc aatagacgga gagactggcc atgacacatg ccttgccgct ggatcgactc
    27301 gacgcgctgc gcgcggcgca gcaaaacgcc tacgaccgag cgcccgccat ccgcgacatc
    27361 tacgagcgcg cgcgcatgac gccgcaggac atccgcagtg cggccgactt ggcgcgcctg
    27421 cccgtcacga gcaaggacgc gctgctggac cttcagcgca aggcgccgcc ttttggcggt
    27481 ttcctggccg ccgacgaagc ggcgatacgg cgcatttttg tgtcacctgg ccccatctac
    27541 gagccacagt tgaacgacga tcaagacggt cacggttttt cccacggctt ccggcgtgcg
    27601 ggcatcggtc cccaggaccg tgtgctgaac acctggtcgt accacttggt gcccgcaggt
    27661 gtcctgctcg accaaggcat cgtggccacg ggcgccaccg tcatcccctg cggcccgggg
    27721 ggcgccgaac agcaggccca actgatcctg gaattgggtg tcacctgcat ctgcgcctcc
    27781 accgccttct tcgtcacgct ggtcaacacc ctggagcaca tgggccacca gcttcccggc
    27841 gcctggaagg tcaagtcggc gctgctcggt ggggaaatgg gtgactggat gggcaagcgg
    27901 cgtgcgcttg agacgaggta cggcatcaag atcttttctg cgtacgccac cggcgacttc
    27961 ggcctcatcg gctacgagga aacgggtcag gagggctacg ccgtgcagcc ggaaaggctg
    28021 gtccagattt gcgacccgca aacgggcacc ccgttgccct tgggcgaaac cggtgagatc
    28081 gtggtcacca ccttggaccg tggctggccc ctcgtgcgtt ttggcaccgg cgatgtggcg
    28141 agtggaagtg tcgcatcagc cgatggcttt gtgagccaca tcagtctctt gaaaggtcgg
    28201 gtcggacagg ctgtaaaggc ccgagagatc ttcatctatc cacgcaacgt cgaggaggtg
    28261 ttgatgcagg ttccgcatat gcagcgcgct caagtggtgg tcacgaagca ggaacaccgc
    28321 gagacggtca ccctgcgcgg cgtcttgcat ccgggcgttg acgcggctac ggtcgagaag
    28381 gctcttcatg agatcttcaa gcaacgcacc cgtttgaagc tggacgccgt cgaatggata
    28441 tccgccgacg atctggtcat tgatgcacct gtgctggtcg atcggaaata cgcatagctc
    28501 agccgctcaa gctgcacggg cgagagcaag gcatagaggt ccttgccttc ctccacgcgc
    28561 agcgttatgc gttcaccgtc gatatcggtg tccttcaacg cgcaaacatc gctggcgagc
    28621 agtccggccc cataggccac ggaaagcgcc gcttgatact tggggttcgg tgcagctgcg
    28681 atcagacgag cgacttcgtc gcagttcagc accaccggcg gcgggcgtgg ctgaccgggc
    28741 gcaggaattt acaccatggt gggagacagt tggatagcga agatgatgtt cggggcagcg
    28801 caaggtagtc ttcgtccgcg acatggcggt gatgcgccgg ttctacggct cctgctcggc
    28861 tttgtggtgc tgcacgagcc gagcccggct ggatcacctg catactgctg ttcgccatct
    28921 ctgcgttgag acgcggcaat attcaaaaaa gcacacgcaa tcgacagtca aatgacccgt
    28981 cttgcccaat accttgaggc tgccgagcgc gacaacacgc gccgaagcta cgcttctgcc
    29041 atccgccatt tcgagatcga atggaaaggc ctgctgcctt ccacggctga tgccattgcc
    29101 cgctacctgg ccgaccacgc agctaccctg tccatcaaca ccctgcgcca gcggctggcg
    29161 gcgctgtcaa ggtggcacaa cgaccagggc tttcccgatc ccaccaaatc ggccttggtg
    29221 cgccaagtcc tcaagggcat tcgctccgtt cacagcgtgc cagaaaagcg cgcgcgcccc
    29281 ctggaactgg cggtgctcca gcagatcgat cagtggctcg ataccgccat cagcaacgcc
    29341 cagcgctctg gcgaccaact ggctttgctg cgccacacgc gcgaccgcag cctgatgctg
    29401 ctggggtttt ggcgcggctt tcgctccgac gaactggtca acctgcgcgt cgagaacacc
    29461 gaggtcacgc cgggccaggg catggcctgc tacctgggcc gcagcaaaag cgacaggcaa
    29521 ctgcagggcc gcgttttcaa atgcccggcc ctgtcccgct tatgccccgt cacggccttc
    29581 aatggctgga tcggactggc cggtttgacc gaaggcccgg tgttccgcaa gattgaccgc
    29641 tggggccatg ttgccgagga
//
LOCUS       DASRUM010000126         3697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_70337, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000126 DASRUM010000000
VERSION     DASRUM010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70337"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..96)
                     /locus_tag="VFF22_08500"
     CDS             complement(<1..96)
                     /locus_tag="VFF22_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16883.1"
                     /translation="MSDLLPRRLAQLGERANLSLLSQCLHGIEREC"
     gene            complement(331..2670)
                     /locus_tag="VFF22_08505"
     CDS             complement(331..2670)
                     /locus_tag="VFF22_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HZX16884.1"
                     /translation="MLSINNRIAEELGVRPQQVAAAVALLDEGSTVPFIARYRKEVTG
                     SLDDTQLRNLEERLRYLRELDDRRASILASIEEQGKLTPELTREINLAETKTRLEDLY
                     LPYKQKRRTKGQIALEAGLGELADALFNDPTLNPESEAERFIDAEKGFADIKAVLEGA
                     KYILMERFAEDANLLASLRSYLKDNAILSARVVAGKENEGAKFRDYFEHDEKLKGAPS
                     HRALAIFRGRNEGILSASLKVGDELPGGAPSGSMHPCEGMIGERFGISARGRAADKWL
                     GEVVRWTWKVKLYTHLETDLFGELREAAEDEAINVFARNLHDLLLAAPAGPRATLALD
                     PGLRTGCKVAVVDATGKVLETSTVYPHAPRNDWDGTLAILAKLCAKHAVDLISIGNGT
                     ASRETDKLAADLIKKLPGLNLTKVMVSEAGASVYSASELAAKEFPDMDVSLRGAVSIA
                     RRLQDPLAELVKIDPKSIGVGQYQHDVSQLKLARSLDAVVEDCVNAVGVDVNTASAAL
                     LARISGLNTTLAQNIVAFRDANGAFNSRADLLKVPRLGDKTFEQAAGFLRVMNGSNPL
                     DASAVHPETYPLVQRIAADTGRDIRSLIGDSGFLKRLDPKQFTDDKFGLVTIGDILSE
                     LEKPGRDPRPEFKTAEFQEGVETLNDLKLGMMLEGVVTNVTNFGAFVDIGVHQDGLVH
                     ISALSEKFIKDPHEAVKAGDVVKVKVMEVDIPRNRIALSMRMGDTPGEKIEGPRGGAR
                     GGKPAGNAPRSERHSSQEKPAPASGGMAALFANAKQLKK"
     gene            2896..3630
                     /gene="ompR"
                     /locus_tag="VFF22_08510"
     CDS             2896..3630
                     /gene="ompR"
                     /locus_tag="VFF22_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HZX16885.1"
                     /translation="MSSTALPAEGEKILIVDDDARLRRLLERFFDEQGYRVRAVENVE
                     QMDRLLARELFNLVVLDLMLPGEDGLSACRRLREANNQVPIIMLTAKGDEASRIQGLE
                     LGADDYLAKPFNPRELLARIKAVLRRQVPVVPGAPGSEDESVSFGEYELSLATRELKK
                     GDEVHMLTTGEFAVLKALVQHAREPLTRDKLMNLARGREWDALERSIDVQISRLRRLI
                     EPDPSKPRYIQTVWGVGYVFVPDGNK"
BASE COUNT          617 a         1119 c         1245 g          716 t
ORIGIN      
        1 gcattcgcgc tcgataccgt gcaggcattg ggaaagcagg gagaggttgg cgcgctcgcc
       61 aagctgggcc aggcggcggg gtaacaggtc gctcaagatt gcattccttc acgcatcggt
      121 cgcctcaata tggggacgac aaacgtgctc tacaaggggg cgagaaaacc gcgttcagcg
      181 gtcgatagcg ctttataacc cgaggcgggt gaagaaggtt atagctgggc gctgtgcttt
      241 tgcgtcaggg cgacgcggga gagaccgggc gtgcggccct gccgaaagca cggacatacg
      301 gcgtatgtcc gtgtgccagc gggcgttggc ttatttcttc agctgcttgg cattggcaaa
      361 cagcgccgcc atgccgccgc tggccggagc cggcttttcc tggctggaat ggcgttcgct
      421 gcgcggagca ttgccggccg gcttgccgcc acgtgcaccg ccacgcgggc cttcgatctt
      481 ctcgccgggg gtgtcgccca tgcgcatcga cagggcgatg cggttacgcg ggatgtcgac
      541 ttccatgacc ttgaccttga ccacgtcgcc agccttgacc gcttcgtgcg ggtccttgat
      601 gaacttctcc gacagcgccg agatatgcac caaaccgtcc tggtgcacgc cgatatcgac
      661 gaaggcgccg aagttggtca cgttggtcac cacgccttcc agcatcatgc ccagcttgag
      721 gtcgttgagg gtttccaccc cttcctggaa ctcggcagtc ttgaactccg gacgcgggtc
      781 gcggccgggc ttttccagct cgctcaagat atcgccgatg gtcaccaggc cgaacttgtc
      841 gtcggtgaac tgcttcgggt cgaggcgctt gaggaagccc gaatcgccga tcagcgagcg
      901 gatatcgcgg ccggtgtcgg cggcgatacg ctgcaccagc gggtaggttt ccgggtgcac
      961 ggcggaggcg tccagcgggt tgctgccgtt cattacacgc aggaagccag cggcctgttc
     1021 gaaggtcttg tcgccgaggc gcggcacctt gagcaggtcg gcgcggctgt tgaacgcgcc
     1081 gttggcgtcg cggaaggcga cgatattctg cgccagggtg gtgttcagcc cggagatgcg
     1141 cgccagcagg gcggcggagg cggtgttcac atcgacgccg acggcgttta cgcagtcctc
     1201 gaccaccgca tccagcgagc gcgccagttt cagctgcgac acgtcgtgct ggtactggcc
     1261 aacaccgata gatttcgggt cgatcttcac cagctcagcc agcgggtctt gcaggcgacg
     1321 ggcgatggat acggcgccgc gcagcgacac gtccatatcc gggaactcct tggcggccag
     1381 ttctgaggcg gaatacaccg aggcgccggc ttcactgacc atgactttgg tcagattcag
     1441 gcccggcagc ttcttgatca gatcggcagc cagcttgtca gtctcacggc tggcggtgcc
     1501 gttgccgatg gagatcaggt cgactgcgtg cttggcgcac agcttggcga ggatcgccag
     1561 ggtgccgtcc cagtcgttgc gcggcgcatg cgggtaaacg gtggaggttt ccagcacctt
     1621 gccggtggca tccaccaccg cgactttgca gccggtacgc aggcccgggt ccagcgccag
     1681 ggtagcgcgc gggccggcgg gcgcggccag cagcaggtcg tgcaggttgc gggcgaacac
     1741 gttgatcgct tcgtcttcgg cggcttcgcg cagttcgccg aacagatcgg tttccaggtg
     1801 ggtgtagagc ttgaccttcc aggtccagcg caccacttcg cccagccatt tgtcggcggc
     1861 gcggccacgg gcgctgatgc cgaagcgctc gccgatcatg ccttcacacg ggtgcatgct
     1921 gcccgacgga gcgccgcccg gaagctcgtc gccgactttc aggctggcgc tcaaaatgcc
     1981 ttcgttacgc ccacggaaga tcgccagggc gcggtgcgac ggtgcgccct tgagcttctc
     2041 gtcgtgctcg aagtaatcgc ggaacttggc gccttcgttc tctttgccgg cgactacgcg
     2101 ggcgctgagg atggcgttgt ccttgaggta gctgcgcagg ctggccagca ggttggcgtc
     2161 ctcggcgaag cgctccatca ggatgtactt ggcgccttcg agcacggcct tgatgtcggc
     2221 aaagcctttt tcggcgtcga taaaacgctc ggcttcgctt tctggattca gggttgggtc
     2281 gttgaacagt gcgtcggcca gctcgccgag gccggcttcc agggcaatct ggcccttggt
     2341 gcggcgcttc tgcttataag gcaggtagag gtcttccagg cgggtcttgg tctcggccag
     2401 gttgatctcg cgggtcagtt caggggtcag cttgccctgt tcctcgatgc tcgccaggat
     2461 gctggcacgg cggtcatcga gttcgcgcag gtagcgcagg cgctcttcca ggttgcgcag
     2521 ctgagtgtca tccaggctgc cggtgacctc tttgcggtag cgcgcgataa acggcacggt
     2581 ggagccttca tcgagcagcg ctacggcggc ggcgacctgt tgcgggcgta cgcccagttc
     2641 ttcggcgatg cggttgttga tgctgagcat ataaaaagct acccacattg aaacgaaaaa
     2701 ccggccacgt agaccacggg ccagacacga aagccgcgca ttataaacac tgcccggcag
     2761 tgcgccggga accagtcggg gaaaaatctg ctaacaatgg gctgcacgtc ggctgcggct
     2821 cctgagccat aatgccggcc attgcaccct gccagcgcgt ccgcgcccgg cggatgcccg
     2881 gacaaggagc cctgaatgag cagcactgca cttcccgctg aaggcgaaaa aatccttatc
     2941 gtcgatgacg atgcccgttt acgccgcctg cttgagcgct tcttcgacga gcagggctac
     3001 cgcgtgcgtg cggtggagaa cgtcgagcag atggaccgtc tgctggcgcg cgagctgttc
     3061 aatctggtgg tgctcgacct gatgctgccg ggcgaggacg gcctgtccgc ctgccgccgc
     3121 ctgcgcgagg cgaacaacca ggtgccgatc atcatgctca ccgccaaggg cgatgaagcc
     3181 agccgtattc agggcctgga actgggcgcc gacgattacc tggccaagcc gttcaaccca
     3241 cgcgagctgc tggcgcggat caaggccgtg ctgcgccgcc aggtcccagt ggtgccaggt
     3301 gcgccgggta gtgaagatga aagcgtgagt ttcggcgagt acgaactgtc cctggccacc
     3361 cgcgagctga agaagggtga cgaggtgcat atgctcacca ccggcgagtt cgctgtgctc
     3421 aaggccctgg tgcaacacgc tcgcgagccg ctgacccgcg acaagctgat gaacctggcc
     3481 cgtggccgtg agtgggacgc cctggagcgc tccatcgacg tgcagatctc gcggctgcgc
     3541 cggctgatcg agccggaccc gtccaagcca cgctatatcc agacggtctg gggcgtgggt
     3601 tatgtgtttg tgccggatgg caataagtga catggcgcgt gtttctcgcg gcgtgtgtgc
     3661 agacgttgtt gctaagcgct gcggctgttc tgttgtt
//
LOCUS       DASRUM010000127        10112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1244839, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000127 DASRUM010000000
VERSION     DASRUM010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 10112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10112
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244839"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VFF22_08515"
     CDS             <1..676
                     /locus_tag="VFF22_08515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HZX16886.1"
                     /translation="LQHQQLESDNDSEYDLRSYLLSAFSQYQKERLWADASLSLGQLD
                     YSDLNRQFALGIAERREKGDTDGKLYALSARIGYDLANAGTDWRVSPFISADLARVDV
                     DGYAEQGNSSTALNFGDQQRDSRRLGVGLQLNYQLSNSTQLYAEVAREREFEDDRRDL
                     KMGLNSVSGNQFELQGHTPANGQTLASFGVQHVLGNELKLSAGYNFRGTTDRQHGINL
                     GLSWDW"
     gene            complement(711..1112)
                     /locus_tag="VFF22_08520"
     CDS             complement(711..1112)
                     /locus_tag="VFF22_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16887.1"
                     /translation="MFCTLLVGCGGKNDPQAALEAAVQQLQDNLEAKKNSAVLDQLHP
                     QFKAQDEFDREWAQRTMLLLFMRHKNVEILALSKVSAVNDTYREKGHTEAQVALIGAE
                     SLIPDSARHFTVEMEWWLEGDEWKLARLNWQ"
     gene            1256..2227
                     /gene="pip"
                     /locus_tag="VFF22_08525"
     CDS             1256..2227
                     /gene="pip"
                     /locus_tag="VFF22_08525"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003249260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HZX16888.1"
                     /translation="MQTLYPEIKPYARHELAVQTPHVLYVDESGSPDGLPVVFIHGGP
                     GAGCDAASRRYFDPNLYRIVTFDQRGCGRSTPHASLENNTTQALIADMEQIREHLGID
                     KWVLFGGSWGSTLALAYAQAYPQRVHGLILRGIFLCRPQEIKWFYQEGASRLFPDYWE
                     DYLTPIPADERGDLVQAFYKRLTGPDQIAQMHAAKAWSTWEGRTATLRPNPQVVDRFS
                     DSHRALSIARIECHYFVNNAFLETDQLLRDMPKIAHLPGVIVHGRYDVICPLDNAWAL
                     HQAWPNSELQIIRDAGHSAAETGITDALVRAADELARRLLNLAPEEA"
     gene            2224..2661
                     /gene="dtd"
                     /locus_tag="VFF22_08530"
     CDS             2224..2661
                     /gene="dtd"
                     /locus_tag="VFF22_08530"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HZX16889.1"
                     /translation="MKALIQRVRGARVEVAGEVVGAIDQGLLALVGVEPQDTPASVDK
                     ILHKLLNYRVFSDADGKMNLSLSDVGGGLLLVSQFTLAADTKSGLRPSFSSAAPPALG
                     AELFDLLVAQARAKHPQVATGQFGADMQVHLVNDGPVTFLLES"
     gene            complement(2658..3245)
                     /locus_tag="VFF22_08535"
     CDS             complement(2658..3245)
                     /locus_tag="VFF22_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HZX16890.1"
                     /translation="MSKTLEFFFDFGSPASYLAWTQLPRIAQEAGAQIVWRPMLLGGV
                     FKATGNQSPVMIPSKGAWMLKDLARFASRYGVPMAFSPSFPINTLTLMRGAAGYQGSE
                     RFEHYVKTIFEALWVQQKNLGKPEVVAEVLSAAGFEPAEFERLVSDEAVKERLKATTE
                     EAVKRGVFGAPTFFIGSEMHFGQDRLDFVAEALAR"
     gene            complement(3292..4020)
                     /locus_tag="VFF22_08540"
     CDS             complement(3292..4020)
                     /locus_tag="VFF22_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX16891.1"
                     /translation="MSERKAILVVGAGDATGGAIAKRFAREGYVACVARRNAEKLGPL
                     VADIEAAGGSARAFGCDARVEEQVIELFAIIEREVGPLHAVVFNIGANVQFSITETTE
                     RVYRKVWEMAALAGFLTGREAAKVMLPRGQGTIIFTGATASLRGRANFSAFASAKFAL
                     RALAQSMARELGPQGIHVAHPIIDGAIDTAFIRDTFPQLYQLKDQDGILNPEHIAETY
                     WQIHCQPRDCWVHELDLRPWSETF"
     gene            4121..4624
                     /locus_tag="VFF22_08545"
     CDS             4121..4624
                     /locus_tag="VFF22_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX16892.1"
                     /translation="MRWEELEQQPCSLARTLSVVGDRWTLLVLRESFLGIRRFDEFEK
                     RLGITRHVLAERLKKLVEAGVLDKVAYQQRPLREEYCLSAKGRDLYPAIMALVNWGDR
                     HMSGPEGAPIRHIHRTCGQPMHGVLVCSDCGEPLQPRDVQLEEAPAFKGQLLPEHWHV
                     LEAGESL"
     gene            complement(4705..5412)
                     /locus_tag="VFF22_08550"
     CDS             complement(4705..5412)
                     /locus_tag="VFF22_08550"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HZX16893.1"
                     /translation="MNLLLLEDGDFIAADRVLLSGRRLKHLHEVHRAEAGDSLRVGRL
                     NGLMGSGQLLRLDAEHAELSIQLDQPPPEKLPLTLMLALPRPKMLRRVLQTVSSMGVP
                     RLILLNSYRVEKSFWQTPFLEHAAIREQLILGLEQSRDTVLPEVIIEKRFKPFVEDQL
                     PGIAAGTMGLVGHPGDFPACPRAVEQAVTLAIGPEGGWIPYEVEKLQEAGLQPVQLGA
                     RILRVETAVTALLARLF"
     gene            complement(5409..6215)
                     /gene="tatC"
                     /locus_tag="VFF22_08555"
     CDS             complement(5409..6215)
                     /gene="tatC"
                     /locus_tag="VFF22_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZX16894.1"
                     /translation="MSQRPDSDQEMPLVSHLTELRTRLLRIVAAVFALFAGLFYFSQD
                     IYALVAAPLRAYLPEGATMIATGVASPFLTPFKLTLMVALFLAIPIILHQIWGFIAPG
                     LYKHEKRIAVPLLISSILLFYAGMAFAYFVVFPIMFGFFASVTPEGVAMMTDIGQYLD
                     FVLTLFFAFGVAFEIPIATFLLIWIGVVDVATLRKSRPYVVVGCFVVGMVLTPPDVFS
                     QTLLAVPMWMLFEVGLFCGSLVKRRAEQDAAAEEAENDSPSNDQPPAAQP"
     gene            complement(6212..6565)
                     /gene="tatB"
                     /locus_tag="VFF22_08560"
     CDS             complement(6212..6565)
                     /gene="tatB"
                     /locus_tag="VFF22_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HZX16895.1"
                     /translation="MFDIGFTELLLVGIVALVVLGPDRLPGAVRTAGLWIGRIKRSFS
                     AIKAEVEREIGADEIRRQLHNEQILELEREMNAMKQSLSAPLSSITEPSPAAAPAAEP
                     AAATDSTRQDKSPQP"
     gene            complement(6569..6799)
                     /gene="tatA"
                     /locus_tag="VFF22_08565"
     CDS             complement(6569..6799)
                     /gene="tatA"
                     /locus_tag="VFF22_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZX16896.1"
                     /translation="MGFGGISIWQLLIVLLIVVMLFGTKRLKGLGSDLGDAIKGFKKS
                     MGNEEDKPAVEEPKGQTIDAQARKVEEPAKKD"
     gene            complement(6970..7302)
                     /locus_tag="VFF22_08570"
     CDS             complement(6970..7302)
                     /locus_tag="VFF22_08570"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020293515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HZX16897.1"
                     /translation="MTDTLSRLAEVLESRKGAAADSSYVASLYHKGLNKILEKVGEES
                     VETILAAKDAAVSGDCSDVIYETADLWFHSLVMLAALGQHPQAVLDELDRRFGLSGHA
                     EKAARSQN"
     gene            complement(7325..7726)
                     /gene="hisI"
                     /locus_tag="VFF22_08575"
     CDS             complement(7325..7726)
                     /gene="hisI"
                     /locus_tag="VFF22_08575"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HZX16898.1"
                     /translation="MTDWLDEINWNSDGLVPAIAQDHKTGRVLMMAWMNREALALTAA
                     EQRAIYWSRSRGKLWRKGEESGHVQKLHELRLDCDADVIILMVEQLGGIACHTGRESC
                     FYRVYDNAEWKTVDAVLKDPHAIYAAGGSHE"
     gene            complement(7794..9383)
                     /gene="ubiB"
                     /locus_tag="VFF22_08580"
     CDS             complement(7794..9383)
                     /gene="ubiB"
                     /locus_tag="VFF22_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HZX16899.1"
                     /translation="MKLLAVRRLLRIQRVVIRYQLDDLLFALPLPFWARALRYVLPWR
                     WLPRRDLLLSRGARLRLALEELGPIFIKFGQLLSTRRDLLPTDIADELAKLQDQVPPF
                     DPAKALALIESQLGAKVGEVFARFDTQPLASASVAQVHAAQLKSGEEVVVKVIRPGLK
                     PVIEQDIAWLFLLAKLAERASADARRLRPVEVVSDYEKTIYDELDLLREAANASQLRR
                     NFEGSPLLYVPQVYWDYCRPKVLVMERIYGIQVTDLETLADQRTDMKLLAERGVEIFF
                     TQVFRDSFFHADMHPGNIFVSTRTPWNPQYIAIDCGIIGSLTAEDQDYLARNLIAFFK
                     RDYRRVAQLHIDSGWVPAETKINDFEAAIRTVCEPIFEKPLKDISFGQVLLRLFQTAR
                     RFNMEVQPQLVLLQKTLLNIEGLGRQLYPDLDLWSTAQPFLERWMRERISQLQLLRNL
                     QLQAEQVPHLSQIARETLERLNQQPTSRQEQPAPSRQWPARLLGAALIAGAATQGLAL
                     SLIVWPSWLMLAGGLYLVLRR"
     gene            complement(9380..9997)
                     /locus_tag="VFF22_08585"
     CDS             complement(9380..9997)
                     /locus_tag="VFF22_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HZX16900.1"
                     /translation="MLLTGLLAGVELGLNRVLAMDSTALPRLARLSGRVIAVECAAPN
                     LQLFILPSANGLQLAAQWAGEADCRLRAPASSLLRLATSKDKTSILHSPDVSLDGDSA
                     ALLELAGILQDLELDWEYELSRWLGPVGSQLLGGHLRSRVNWSSQALDSLRLNLADYL
                     SEESRSLVGQREADARFAELDHLKLSLDRLDARIERLAQRHKPIA"
     gene            complement(9997..>10112)
                     /locus_tag="VFF22_08590"
     CDS             complement(9997..>10112)
                     /locus_tag="VFF22_08590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX16901.1"
                     /translation="HPDQDTLKAMMVEAGFERVTFHNMTGGIVALHRGIKP"
BASE COUNT         1851 a         3021 c         3204 g         2036 t
ORIGIN      
        1 cttacagcat cagcaattgg agagcgacaa cgattccgag tacgacctgc gcagctattt
       61 gctcagcgcc ttttcccagt accaaaagga gcgtctgtgg gctgatgcca gcctgagtct
      121 ggggcagctg gactacagcg atctcaaccg tcagtttgct ctgggtattg ccgagcgccg
      181 cgagaaaggc gataccgatg gcaagctcta tgcgctgtct gcgcggattg gctatgacct
      241 ggccaatgcc ggcactgact ggcgtgtcag tccctttatc agtgccgatc tggcacgggt
      301 ggatgtggat ggttatgccg agcagggcaa cagttccacg gcactcaact ttggcgatca
      361 gcagcgtgac agtcgccgct tgggtgtagg cttgcagctt aattatcaat tgagcaacag
      421 cacccagctg tatgccgagg tcgcccgtga acgcgagttc gaggacgatc ggcgtgactt
      481 gaaaatgggg cttaactccg tgtcgggcaa tcaattcgag ctgcaggggc atacgccagc
      541 caatggacag accttggcca gcttcggggt acagcatgtg cttgggaatg agttaaagct
      601 gagcgcgggc tacaacttcc gtggcactac agaccgccag cacggcatta accttgggct
      661 cagctgggat tggtagtgct cttagccgcc cgcagcttgt ctgcgggtgg ttattgccag
      721 ttaagtcgcg ccagtttcca ctcatcacct tccagccacc actccatctc cacggtgaag
      781 tggcgggcac tatctgggat cagactttcg gcaccgatca gtgccacctg agcttcggtg
      841 tgacctttct ctcggtaggt gtcattgacg gcgctgacct tgctcagggc cagtatctcg
      901 acgttcttgt ggcgcataaa cagcagcaac atggtgcgct gtgcccattc gcggtcgaac
      961 tcgtcctggg ctttgaactg cgggtgtagt tggtcgagca ccgcgctgtt cttcttcgct
     1021 tccaggttgt cctgcagttg ttgcacggcc gcttccagcg ctgcttgcgg atcgttcttg
     1081 ccgccgcaac cgacgagcag ggtgcaaaac attacgaaag ctaaggcaaa tatccttgtt
     1141 ggcatgctga actccttttt catggttatg atgcccggca gagcgttaaa cggttgcggt
     1201 cggcggatgg cttgcgccaa gcagtcgacg atacgcatca attcaggagc atgcaatgca
     1261 gaccttgtat ccggagatta aaccctacgc acgccatgag ctggcggtgc aaacgccgca
     1321 cgtgctgtat gtcgacgaga gtggttcgcc ggatggtttg cccgtggtgt ttatccatgg
     1381 tggcccgggg gctggctgtg atgctgccag ccggcgctat ttcgacccta acctgtatcg
     1441 catcgtcacc ttcgatcagc gcggttgtgg tcgttccacg ccccacgcca gcctggaaaa
     1501 caacacgact caagcgctga tcgccgacat ggagcagatc cgcgagcacc tcggcatcga
     1561 caagtgggtg ctgttcggcg gctcctgggg ctcgaccctg gccctggctt acgcccaggc
     1621 ctatccgcag cgtgtgcatg ggctgatcct gcgcggcatc ttcctctgcc gcccgcagga
     1681 aattaagtgg ttctatcagg agggtgcgag ccgcctgttc cccgactact gggaagacta
     1741 cctgacaccg atccctgctg atgagcgtgg cgatctggtg caggccttct acaagcgtct
     1801 gaccgggccc gatcagatcg cccagatgca tgcggccaaa gcctggtcga cctgggaagg
     1861 ccgtaccgcg accttgcgtc ccaatccgca ggtggtcgac cgtttcagcg acagccaccg
     1921 tgcgctgtcg atcgcccgta tcgaatgcca ttacttcgtt aacaatgcct tcctcgaaac
     1981 cgatcagctg cttcgcgata tgccgaagat cgcccacctg cccggcgtga tcgtgcatgg
     2041 ccgctatgac gtgatctgcc cgctggataa cgcctgggcg ctacaccagg cctggccaaa
     2101 cagtgagctg cagattattc gcgatgccgg ccattcggcg gccgaaaccg gtattaccga
     2161 tgccctggtg cgtgcggcgg atgagctggc gcggcgtctg ctcaacctgg ctccggaaga
     2221 agcatgaagg cactgattca acgggtgcgt ggcgcccggg tagaggtcgc gggtgaggtg
     2281 gttggcgcga ttgatcaggg cctgctggcg ctggtcggcg tcgagccgca ggacactcct
     2341 gccagtgtcg ataaaatcct gcacaagctg ctcaactacc gggtgttcag cgatgccgac
     2401 ggcaagatga acctgtcgct cagtgacgtc ggcggcggcc tgttgctggt gtcgcaattc
     2461 accctggccg ccgataccaa aagcggcctg cgcccgagct tttccagtgc cgcgccgccg
     2521 gcgctgggcg cggaactgtt cgacctgctg gtcgctcagg cccgcgccaa acacccgcag
     2581 gttgccaccg gccagttcgg cgccgacatg caggtgcatc tggtcaatga tgggccggtg
     2641 acctttctgc tggaaagcta gcgcgccagg gcttcggcga cgaagtccag gcgatcctgg
     2701 ccgaagtgca tctcgctgcc gataaagaag gtcggtgcgc caaatactcc gcgtttgacc
     2761 gcttcctcgg tggtggcctt gaggcgttcc ttgaccgctt catcgctgac caggcgctcg
     2821 aactcggccg gctcgaagcc ggctgcgcta agcacttcgg caaccacttc cggcttgccg
     2881 aggtttttct gctgcaccca cagcgcttcg aagatggttt tcacgtagtg ctcgaagcgc
     2941 tcgcttccct ggtaaccggc cgcgccgcgc atcagcgtca gggtgttgat cgggaagctc
     3001 gggctgaagg ccatcggcac accgtagcgg ctggcaaagc gcgccaggtc cttgagcatc
     3061 caggcgcctt tgctcgggat catcaccggt gactggttgc cggtggcctt gaatacgccg
     3121 ccgagcagca tcggtcgcca gacgatctgc gcaccggcct cttgggcaat tcgcggtaac
     3181 tgggtccagg ccaggtagct ggctgggctg ccgaaatcga aaaagaactc tagggttttg
     3241 ctcattgcat actccttcgg gaggtcttgt tattgggcga gcgcgcagct gctagaacgt
     3301 ctcagaccag ggccgcagat ccagttcatg cacccagcaa tcgcggggct ggcagtggat
     3361 ctgccagtag gtctcggcga tatgctcggg gttgaggatg ccgtcctggt ccttgagctg
     3421 gtacagctgc ggaaaggtgt cgcggataaa cgcggtgtcg atggccccgt cgataatcgg
     3481 gtgggcgaca tgaatgccct gggggccgag ttcacgggcc atgctctggg ccaatgcgcg
     3541 cagggcgaac ttggcgctgg cgaaggcgga gaagttggcg cggccacgca aggaggcggt
     3601 ggcaccggta aaaatgatcg tgccctggcc gcgcggcagc atcaccttgg cggcttcacg
     3661 accggtgaga aagccggcca acgcggccat ttcccagacc ttgcggtaga cccgttcggt
     3721 ggtctcggtg atggagaact gcacgttggc gccgatgttg aacaccaccg cgtgcagcgg
     3781 gccgacttcg cgctcgatga tggcaaacag ttcgatgacc tgttcctcga ctcgcgcatc
     3841 gcagccaaag gccctcgcgc tgccaccggc tgcctcgata tccgcaacca gcggacctag
     3901 tttctcggcg ttgcgccgcg ccacgcaggc gacatagccc tcgcgggcga agcgtttggc
     3961 gatggcgccg ccggtggcat caccggcgcc gactaccaga attgctttgc gttcgctcat
     4021 tgcagtctcc gtcagttcta ttttggaacc tggcttgata gtcggttcca ttttggaatc
     4081 tgtcaagcta tggtgagcca tcgcggttgg agattggctt atgcgttggg aagaattgga
     4141 gcagcaacct tgctccctgg cacgcaccct gtcggtggtg ggtgaccgtt ggacgctgct
     4201 ggtgctgcgc gagagttttc tcggtattcg tcgtttcgat gaatttgaga aacgcctggg
     4261 gattacccgt cacgtgctgg ccgagcggct gaaaaagctg gtggaagctg gggtgctgga
     4321 caaggtggcc tatcagcagc ggccgttgcg tgaggaatac tgcctgagcg ccaagggccg
     4381 cgatttgtac ccggccatca tggccctggt gaattggggc gaccgccata tgagcggccc
     4441 ggaaggcgcg ccgatccggc atatccaccg cacttgcggc cagccgatgc atggcgtgct
     4501 ggtgtgttcg gattgtggtg agccgctgca gccgcgtgac gttcagttgg aggaagcgcc
     4561 tgcgttcaag gggcagttat tgcctgagca ttggcacgtt cttgaagctg gagagagcct
     4621 gtaggagcac gccatgcgtg cgagcgattt tcctgatacc actattcgcg ggcatggccc
     4681 gctcctacaa agccgcagct ttggttaaaa caagcgcgct agcaaggcgg tcaccgccgt
     4741 ttccactcgc agaatccgcg cgcccagttg caccggttgc aggccagctt cctgcagttt
     4801 ttccacttcg tagggaatcc agccgccttc cgggccgatg gccagcgtca cggcctgttc
     4861 gactgcccgt gggcaggccg ggaagtcgcc ggggtggccg acgagaccca tggtaccggc
     4921 tgcgatgcca ggcagctggt cttcgacaaa gggcttgaag cgtttctcga taatcacttc
     4981 cggcagcacg gtatcgcggg actgttccag gccgaggatc agctgttcgc gaatcgctgc
     5041 gtgctccaga aaaggcgtct gccagaagct tttttccacc cggtagctgt tcagcagtat
     5101 tagccgaggc acgcccatgc tgctgacggt ttgtagcacg cgacggagca tcttcggtcg
     5161 gggcagggcc agcatcaggg tcagtggcag tttttccggt ggtggttggt cgagctggat
     5221 cgacagttcg gcatgctcgg catccagacg tagtaactgg ccgctgccca tcaggccatt
     5281 caagcggcca acacgcaggc tgtcgccggc ttcggcgcga tgaacttcat gcaggtgctt
     5341 gaggcggcgg ccgctgagca gcacacggtc ggcggcgata aagtcgccgt cttccagtag
     5401 cagcaggttc acggttgcgc ggcgggcggc tggtcgttgg aggggctgtc gttttcggct
     5461 tcttcagccg ccgcgtcctg ttcggcgcgg cgcttgacca gactgccgca gaacagaccg
     5521 acctcgaaca gcatccacat gggcacggcc agcagggtct gcgagaacac gtcaggtggg
     5581 gtcagtacca tgccgacgac aaagcagccg accaccacat aggggcggct cttgcgcagg
     5641 gtggcgacat cgaccactcc gatccagatc agcaaaaagg tggcaatcgg gatttcgaac
     5701 gcgacgccga aggcgaagaa cagcgtcagt acaaagtcca ggtactgacc gatgtcggtc
     5761 atcatcgcca cgccttccgg ggtcacgctg gcgaagaagc cgaacatgat cgggaacacc
     5821 acgaagtagg cgaaggccat gccggcgtag aacagcagga tgctggagat cagcaacggt
     5881 acggcaatgc gcttctcatg cttgtacagc cctggcgcga taaagcccca gatctgatgc
     5941 aggatgatcg ggatggccag aaacagcgcg accatcagcg tcagcttgaa cggcgtgaga
     6001 aacggcgagg ccacacccgt ggcgatcatc gttgcgcctt ccggcaggta ggcgcgcagc
     6061 ggcgcggcga ccagcgcgta gatgtcctga gagaaataga acagcccggc aaacagcgcg
     6121 aataccgccg ccacaatacg cagcaagcgc gtgcgcagtt cggtcaggtg ggagaccagg
     6181 ggcatttcct gatcgctgtc cggtcgttgg ctcatggttg cgggctttta tcctgacggg
     6241 tgctatcggt agctgctgcc ggctctgcgg cgggtgcagc ggcagggctc ggttcggtga
     6301 tgctgctaag cggcgcgctg aggctttgct tcatcgcgtt catttcccgc tccagctcga
     6361 gaatctgctc gttatgcagt tggcggcgga tttcatccgc gccgatctcg cgctcgacct
     6421 ccgccttgat cgcactgaag ctgcgcttga tccggccgat ccacaaaccc gctgtgcgca
     6481 ccgcacccgg cagacgatca ggccccagca ccaccagggc gacgatgccg accagcagca
     6541 gctcggtaaa gccgatatcg aacatggctt agtctttttt cgccggttct tcgaccttgc
     6601 gtgcctgagc gtcgatggtc tggcccttgg gctcttcgac ggcaggtttg tcttcttcgt
     6661 tgcccatgga cttcttgaaa cccttgatcg cgtcgcccag gtccgaaccc aggcccttca
     6721 ggcgcttggt gccgaacagc atgaccacga tcagcaggac gatcaggagt tgccagatgc
     6781 taatgccacc gaatcccata gtgcgtactc cgtagtaaga ggttgatcag agggtgttta
     6841 caaattactg cgctcggcca ttctgcgttg aaatcagcct caaaatgctc atgtacagct
     6901 cgtacactgc gctttttcgg ctaatttcgc cttgcctgac catcgctcgc tacttttgta
     6961 aacgccctct cagttttgcg agcgcgcggc tttttccgcg tgcccggaca agccgaaacg
     7021 ccgatccagt tcgtccagta ccgcctgagg gtgctggccg agagcggcga gcatgaccag
     7081 gctgtggaac cacaggtcgg cggtttcata aatcacatcg ctgcagtcac cgctgacggc
     7141 agcatccttg gcggcgagaa tggtttccac cgattcctca ccgacctttt ccagaatctt
     7201 gtttaggccc ttgtggtaca ggctggcgac ataggagctg tcagcggccg cgcctttgcg
     7261 cgactccagc acttcggcca agcggctcag ggtatcagtc atgcccgtgt ctctcatgtc
     7321 cgcgctactc atgactgcct cctgcggcat agatggcgtg cgggtctttc agtaccgcgt
     7381 cgacggtctt ccactcggcg ttgtcgtata cccggtagaa gcagctttcg cggccggtgt
     7441 ggcaggcgat cccgcccagt tgttcgacca tcaggatgat cacatcggcg tcgcaatcca
     7501 ggcgcagctc gtgcagcttc tgcacatggc cggattcctc gcccttgcgc cacagcttgc
     7561 cacgcgagcg tgaccagtag atggcgcgct gctcggcggc agtcagggcc agggcctcac
     7621 ggttcatcca ggccatcatc agcacgcggc cggtcttgtg gtcctgggcg attgccggca
     7681 ccaggccgtc gctgttccag ttgatttcgt cgagccagtc agtcattgcc gtatccgccc
     7741 atctcgggct gcttgattca gaaagctagt gtgccagtgc gcacccggtg tggctatcgg
     7801 cgcagcacca gatagaggcc accagcgagc atcagccagc tcggccagac gatcaggctc
     7861 aatgccaggc cttgcgtagc tgcgccagcg attagcgctg cgccgagcag gcgggccggc
     7921 cattggcggc tcggtgcagg ctgttcctgg cggctggtcg gctgctggtt taggcgctcc
     7981 agggtttcgc gggcgatctg cgacaagtgc ggcacctgct cggcctgtag ttgcaggttg
     8041 cgcagcagct gcagctggct gatgcgctca cgcatccagc gctcgagaaa cggctgcgcg
     8101 gtgctccaca gatcgaggtc cgggtacagc tggcggccca ggccttcgat attcagcagg
     8161 gttttctgca gcagcaccag ctgcggctgc acttccatat tgaagcgtcg cgcagtctgg
     8221 aacagacgca acagcacctg gccgaaggag atgtccttca gtggtttttc gaagatcggc
     8281 tcgcacacgg tgcggatcgc cgcctcgaaa tcattgatct tggtttccgc aggcacccag
     8341 cccgagtcga tatgcagctg cgccacgcga cggtaatcgc gcttgaagaa ggcaatcagg
     8401 ttgcgcgcca ggtagtcctg atcttcggcg gtgaggctgc cgatgatgcc gcagtcgatg
     8461 gcgatgtatt gcgggttcca cggcgtgcgg gtgctgacga agatattgcc ggggtgcatg
     8521 tcggcgtgga agaaactgtc gcggaacacc tgggtgaaga aaatctccac accgcgttcg
     8581 gccaacagtt tcatgtcggt gcgctgatcg gccagggttt ccaggtcggt gacctgaata
     8641 ccgtagatgc gctccatcac cagcactttc ggccggcaat agtcccagta cacctgcggc
     8701 acatagagca gcggcgagcc ttcgaagtta cggcgcagct ggctggcgtt ggccgcttcg
     8761 cgcagcaggt cgagttcgtc gtagatggtc ttttcgtagt cgctgaccac ttcaaccggg
     8821 cgcaggcggc gggcatccgc ggaggcgcgc tcggccagtt tggcgagcag aaacagccag
     8881 gcaatatcct gttcgataac cggtttcaga cccgggcgga tcaccttgac caccacctct
     8941 tcgccgcttt tcagctgcgc ggcatgcacc tgggcgacgg aggccgaggc cagcggttga
     9001 gtgtcaaagc gggcaaacac ctcaccgacc ttggcgccca gttgcgattc gatcagcgcc
     9061 agcgccttgg ccggatcgaa gggcggcacc tggtcctgca acttggccag ctcatcggcg
     9121 atatcggttg gcagcagatc gcggcgggtg gaaagcagct ggccgaactt gataaaaatc
     9181 ggccccagct cttccagcgc tagacgcagg cgtgcaccgc gcgacagcag caagtcgcgg
     9241 cgtggcagcc agcgccaggg caacacataa cgcagcgcgc gtgcccagaa gggcagcggc
     9301 agggcaaata acaggtcgtc cagctggtag cgaatgacta cgcgctggat acgcaacaga
     9361 cgacgaacgg caagcagctt catgcgatgg gcttatggcg ttgggcgagg cgctcgatgc
     9421 gcgcgtcgag tcggtcgagg gacagcttga ggtggtccag ttcggcgaag cgcgcatcgg
     9481 cttcgcgctg gccgaccagg ctgcgggatt cttcactgag gtaatcagcc aggttcaggc
     9541 gcaggctgtc gagcgcctgg ctgctccagt taacccggct gcgcagatgg ccaccgagca
     9601 gctggctgcc gaccgggccg agccagcgcg acagttcgta ttcccagtcc aactcgagat
     9661 cctgaaggat cccggccagt tccagcaggg cggcgctgtc gccatccagg ctgacgtccg
     9721 ggctgtgcag gatgctggtc ttgtctttgc tggtggccag gcgtaacagg ctggatgcgg
     9781 gtgcgcgtaa acggcaatcc gcttcacccg cccactgcgc ggccagttgc aggccgttgg
     9841 cgctgggcag aataaacagc tgcagattcg gcgcggcgca ctcgacggca atcacccggc
     9901 cgctcaatcg tgccaggcgc ggcagcgcgg tgctgtccat ggccaatacg cggttaaggc
     9961 cgagttcgac tccggccagc agcccggtca gcagcatcag ggcttgatgc cgcggtgcag
    10021 ggcgacgatg ccgccggtca tattgtggaa ggtcacgcgc tcgaagccgg cttcaaccat
    10081 catcgccttg agggtgtcct gatccgggtg ca
//
LOCUS       DASRUM010000128        18367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_71194, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000128 DASRUM010000000
VERSION     DASRUM010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 18367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18367
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71194"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..583)
                     /locus_tag="VFF22_08595"
     CDS             complement(233..583)
                     /locus_tag="VFF22_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16902.1"
                     /translation="MDVVKSLKPGKNGTKRFVERYGDDLIAVRYRHDAEKQISYTTIE
                     LIVERKHKPPKTRTQPAGQPTALVLVRIGFHEKDMQLAVKQAGGKWQPEQRAWLLNYK
                     AAVQLGLQERIINS"
     gene            complement(670..1290)
                     /locus_tag="VFF22_08600"
     CDS             complement(670..1290)
                     /locus_tag="VFF22_08600"
                     /inference="COORDINATES: protein motif:HMM:NF036520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZX16903.1"
                     /translation="MRKKPCQQRSQQMVDILLEASGRVLERHGLANLTTNRVADEAGV
                     SVGSLYQYFANKQELLEALLERMALDITRLVDARMAQLLEADVRTAVRMLLSDVLAFI
                     LSGNGRYRVLVRHWQQLQALHVVDRLERYLSEVCRRYLLRHIRQFPIDNPMPMLFVMI
                     NATLMTLVRHLALDEPPLSDQQLIDELTHMLTAYLAAAASPHQTES"
     gene            1370..2224
                     /locus_tag="VFF22_08605"
     CDS             1370..2224
                     /locus_tag="VFF22_08605"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HZX16904.1"
                     /translation="MQMTCPVSDTQTLQPLSPDSLTWRYFGDLRGLLLISRVGLLQNL
                     HPGLAAGVQEHSDLFVNPWNRLFRSIPPILAVVYGAERAPATGVQIRDWHRQIKGVDA
                     QGRRYHALSPELFYWAHATFFEGQIAAQALFGTPLDDAALERLYQESIQWYALYGLPM
                     TTVPADYKAFKVYWQDMLESRLEMTGVARWYLDGNQPLPAPYTWMRGPLWWLLRPLFD
                     QGARWLAIGSLPAPLRERLKLPWSAKDQRRLDGLSRMVRALWKVMPRRWRYFPQAHAA
                     MRRAGKAG"
     gene            complement(2275..3768)
                     /locus_tag="VFF22_08610"
     CDS             complement(2275..3768)
                     /locus_tag="VFF22_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HZX16905.1"
                     /translation="MKRLLLVVLIALLGLIAVVVGRTLLLPAATFNSAPVSLPEGLDG
                     QRAAESLAAAIRLPTLSHQVGTPASQQAASDAAFAGLREWLAQRYPRLTATTEQLDTG
                     SPSILLRWPGKDSQLPAALLMAHQDVVPVAPGTEELWTHPPFSGAIADGFVWGRGAID
                     SKGSMVAILEAVETLIAQGFQPQRDVLLAFGHDEEIGGHQGNRRIAAQLQEQGKRLAL
                     VSDEGGFVVRGQIPGVSQDVAVVGIAEKGYVSLTLQANAQGGHSSQPPAFDETAIGRL
                     SQALQRVGDAPFQRGFDGPTGDLLASFTPAQSFGYRLIFANLWLFGPLVEQQLAASPA
                     GAAQMQTSLSPTLLRAGIKENVLPPSARATLNLRIHPRDSIESVAEHVRNAVADEQIQ
                     VKVMPAGREPSAVSDVNGAAYKQFAEVIRQSFSNTLVSPNLTVGGTDSRYYEPLTDNI
                     FRFSPLLMEREDLPRMHGTNERVGIDTLANASGFYYRLLQSLNNPAD"
     gene            complement(3786..6161)
                     /locus_tag="VFF22_08615"
     CDS             complement(3786..6161)
                     /locus_tag="VFF22_08615"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZX16906.1"
                     /translation="MKRTLTVLAVLVAATAAGAYWYIDSKQPQRAGELSLSSLQAPVT
                     VDYDERGVPHIQAQNEADMYRALGYVHAQDRLFQMEILRRLARGELAEILGSKLVDTD
                     RLFRTLGIREHADRYAAKMDMNTPASKALQAYLDGINQYQASRPAPIEFDVLGIEKRP
                     FTTADTLSVAGYMAYSFAAAFRTEPVLTHIRDQLGADYLKAFDLDWHPQGVVGQNLAA
                     ADWQDLNAIAELSHAALLNAGLPQFEGSNAWAVSGSRTASGKPLLAGDPHIRFAVPSV
                     WYEANLSYPGFSLYGHHQALNPVASLGHNREFAWSLTMFQNDDLDLIAEKTNPDNPNQ
                     VWYRGQWVELQSRVEQITVKDAAPVSLTIRRSPHGPLINSALGETVGSTPIAMWWAFL
                     ETENPILDGFYQLNRAASLEQARAAVEHIEAPGLNVVWANATGDIGWWAAAKLPRRPA
                     GVNPSFILDGASGEAEKDGYFPFSANPQEENPARGYIVSANYQPVSPTGMEIPGYYNL
                     ADRGQRLDDRLSDSSVKWDLANSQALQLDPGTGYPQRLLAPLAADLRAAVGDPEEQAL
                     VEQLLSWDGQHTLDATAATLFNQLVYQLTFEAMADELGEAFFTNLLQTRVLDSALPRL
                     AADASSPWWDKRGSDAVESRADTVKAAWQASLTHLRSTQGDDATQWTWGSAHTLTHSH
                     PLGQQKPLDKLFNVGPFAAPGGHETPNNLSQPVGPAPWSVVYGPSTRRLIDLADSSTA
                     LGINPVGQSGVLFDKHYKDQAEAYMRGEYVPMHLDAEAIKANSRSQLILRP"
     gene            6276..7325
                     /locus_tag="VFF22_08620"
     CDS             6276..7325
                     /locus_tag="VFF22_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZX16907.1"
                     /translation="METDPHNSIASALTHSSTLRRLLYEALAELGLDPTDTYRQAYQG
                     VVLAPPLLNAREDHDNAPRFWQALAGISGDVDIGLHLGETMQPRPLDVVGYLLLASRD
                     LGQALESFVRFQHILSGGFAARLELDDEQVRLIFDLNYRGVGSLRQQMECLALLLQKM
                     LASINNDGSFRLTGLDFRHPAPRRLSEHRRLFGLLPRFAQAHDALIFPRAWLSRPSRS
                     ANPRLFAVLWAQAEVELAELEENQLLNRVRYWLEVNLGVQLCDLPRCAKALGYSVGGL
                     QRALAEQQQSFRQLHDEVRRLRAQALLDSGLAIREVARACGFAELSPFYRAFKRWQGE
                     TPQGFRRQSDKYGGG"
     gene            complement(7332..7952)
                     /locus_tag="VFF22_08625"
     CDS             complement(7332..7952)
                     /locus_tag="VFF22_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004352065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6436 domain-containing protein"
                     /protein_id="HZX16908.1"
                     /translation="MASCAPVCRRLPMTRPTRKTLLASLLIAAWAAAMLAAYWWYEAR
                     YLRSFSEQTALFYGEQLRLPDELAGPGPIRLVHFWDPACPCNVGNQQHLAELIERFGP
                     QGVTFYAVQKPGSKGQLPTTLSAMQQLTELPGSTQIPASPAVAIWDRNGQLAYFGPYS
                     EGATCTSSNSFIEPILDALIAERPVNASNTLAVGCFCDWSADVAHD"
     gene            8038..8970
                     /locus_tag="VFF22_08630"
     CDS             8038..8970
                     /locus_tag="VFF22_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX16909.1"
                     /translation="MPEPFQPENLIASLRPLAAAAPMSLAEQGYRQFYGFSDGLARHS
                     RLGAVQAAGYQIAVQAWWPEQPRATFVLLHGYYDHSGLYRHVIEWALGMGFAVLSFDL
                     PGHGLSSGARASIGDFAEYQTVLQAVLVEAAALELPQPWHLCGQSTGGAILIDYLLNG
                     EPLTQIGETILLAPLVRPRAWGWSQLSYRVMRNFVSEIPRRFSVNSSDAAFIDFVHHQ
                     DPLQPRNLPTAWVGALSQWVPRIEAAPRSARSPLIIQGEADMTVAWQHNLEVLQNKFA
                     APQILRLPEARHHLANEAPALRQRYFEFLSERLS"
     gene            complement(8971..9738)
                     /locus_tag="VFF22_08635"
     CDS             complement(8971..9738)
                     /locus_tag="VFF22_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16910.1"
                     /translation="MRALSVVLTLLLSLPALADDYQQLYQAAGWPQQRMHFSDALQAA
                     QERYRSSLPPAVHQALVNNSNQRFAAAAVDQRAQQGLRDSLDNPTSALQFFQSPLGRK
                     IVDAELLATRSDQLARYANGLPRSEASATRRLLIRHLAQALPAKEAGAEVSLALAGVA
                     ADSLSQMLPGLLGGDSAQAMLNGQRQRLMEQIGADLDNTLLHVYRELSDPELEEFVSF
                     AQSETGKSYYQAALAAIRSGLAVGQSSASLAPAPQGI"
     gene            9822..10445
                     /locus_tag="VFF22_08640"
     CDS             9822..10445
                     /locus_tag="VFF22_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HZX16911.1"
                     /translation="MTTDTHTACLTRIVDDLAEHGWSLQPQFIAPSLTLELAEECRKR
                     AAQGALAPAGVGRGAQQQIREGVRGDHIQWLEAGQAEVCDQYLQAFDELRVALNQGLY
                     LGLEDFEGHFALYPPGAFYQKHVDRFRDDDRRAVSAVFYLNEGWQAEQGGALRLYLPS
                     GETRDVLPQAGNLLVFLSADMPHEVLPATRDRLSLTGWFRRRGDSVF"
     gene            complement(10474..11034)
                     /locus_tag="VFF22_08645"
     CDS             complement(10474..11034)
                     /locus_tag="VFF22_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6160 family protein"
                     /protein_id="HZX16912.1"
                     /translation="MPTVTYRPLLSALLICLLPLGQAHGEMQALDNASLSAIAGQAGI
                     TLTLDLKTEAAKISWEDDGGSLQLRNLQIDNGCAAPGDCPVNVAYGPAKLYFNSALVN
                     APTLKVDIVNQGGTQKMQLALPDLKTTSDQLLADGLITTPLTIRMRIAADIGIGDAGA
                     SSSSTLGRVEIRDLSNLQGQLRIWGH"
     gene            11268..11780
                     /locus_tag="VFF22_08650"
     CDS             11268..11780
                     /locus_tag="VFF22_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HZX16913.1"
                     /translation="MQKILVSRCLLGHAVRYDGGAHGPFTVLERWQQEGRIVPLCPEV
                     AGGLSTPRAPAEIAGGQGAQVLDGQLPVLTVDGEDVTAAFIAGAEQALALVAQHGIRL
                     ALLKARSPSCGNLEAYDGTFSGVRVPGEGVTAALLRRAGVQVFSEEQLEHALQALVEL
                     EVSEGTSAGV"
     gene            11818..12255
                     /locus_tag="VFF22_08655"
     CDS             11818..12255
                     /locus_tag="VFF22_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HZX16914.1"
                     /translation="MNRRLTSFALGAALLCGTFPSAWAEMPRTAAPDGAAVYFIEPQD
                     GATLEQTFTVKFGLKGMGVAPAGVAVQNTGHHHLLVDVSTLPAMNAPLPMSDQVRHFG
                     AGQTETELSLAPGTHTLQLLLGDQNHVPHEPPVLSERITVTVK"
     gene            complement(12328..13560)
                     /gene="serA"
                     /locus_tag="VFF22_08660"
     CDS             complement(12328..13560)
                     /gene="serA"
                     /locus_tag="VFF22_08660"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004754423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZX16915.1"
                     /translation="MSSKTSLDKSKIKFLLLEGVHQNAVDTLKAAGYTNIEYLKGALS
                     DDELKEKIADAHFIGIRSRTQLTAEVFDCAKRLVAVGCFCIGTNQVDLDAARERGIAV
                     FNAPYSNTRSVAELVLAQAILLLRGIPEKNASCHRGGWIKSAANSFEIRGKKLGIVGY
                     GSIGTQLSVLAEAMGMQVFFYDTVTKLPLGNATQIAKLHDLLGMCDIVSLHVPELPST
                     QWMIGEKEIRAMKKGGILINAARGTVVELDHLAAAIKDEHLIGAAIDVFPVEPKSNDE
                     EFESPLRGLDRVILTPHIGGSTAEAQANIGLEVAEKLVKYSDNGTSVSSVNFPEVALP
                     AHPGKHRLLHIHENIPGVMSEINNVFAENGINISGQFLQTNDKVGYVVIDVDADYSDL
                     ALEKLQHVRGTIRSRVLF"
     gene            13747..15141
                     /locus_tag="VFF22_08665"
     CDS             13747..15141
                     /locus_tag="VFF22_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017139510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZX16916.1"
                     /translation="MTNPALIEELKTLVEPGKVLTDADSLNAYGKDWTKHFAPAPLAI
                     AFPKSIEQVQAIVRWANQHKVALVPSGGRTGLSAGAVAANGEVVVAFDYMNQILDFNE
                     FDRTAVCQPGVVTKQLQMFAEDKGLYYPVDFASAGSSQIGGNIGTNAGGIKVIRYGMT
                     RNWVAGLKVVTGTGELLELNKDLIKNATGYDMRQLFIGAEGTLGFVVEATMRLDRAPK
                     NLTAMVLGTPDFDSIMPVLHAFQNKLDLTAFEFFSDKALAKIMARGDVPAAFETDCPF
                     YALLEFEATTEEVAEAALATFEHCVEQGWVVDGVMSQSEQQLQNLWKLREYISETISH
                     WTPYKNDISVTVSKVPAFLHDIDRIVGEHYPDFEIVWFGHIGDGNLHLNILKPESLSK
                     DEFFAKCATVNKWVFETVQKYNGSISAEHGVGMTKRDYLIYSRSEAEIGYMKAIKAVF
                     DPNGIMNPGKIFPL"
     gene            15304..15969
                     /locus_tag="VFF22_08670"
     CDS             15304..15969
                     /locus_tag="VFF22_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZX16917.1"
                     /translation="MSYQHQYVDGTHIHFPMGKVVCIGRNYAEHAKELNNPVPTEPLL
                     FIKAGSCVVPLDDGFTLVEGKGDVHYEAEIAVLIGKPLSRTPSAEEVRDAISGFAPAL
                     DLTLRDLQATLKAKGYPWETAKSFDGACVLAPFVPGDAVEDLADIGIRLVINGEVRQD
                     GNSRDMLNPILPMIQHICGHFSLQPGDVILTGTPVGVGPLHKGDELVLELVGHSRFES
                     RAL"
     gene            16094..17029
                     /locus_tag="VFF22_08675"
     CDS             16094..17029
                     /locus_tag="VFF22_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HZX16918.1"
                     /translation="MSIWIARLVRLRWLIVLFLLLVIALLVMNYRHWDDRFSFWVQQQ
                     QVDAAEQQASIWLPGYKAVLQGKPLQGLEGDEVSGLTYSPESDTLFTVTGKHPQLIEL
                     TLDGQVMRRIDLHGFSNPEGVEMLANGRLAIIDERKRTLTTFKLGALTRSLEFADLAS
                     FDLGFIDAGNKGFEGIAWDSRNERVLLGKERGPLGLFSLPFPGEDGAAGALQPMSSGR
                     LFLRDISSLTYDPRTGHALVLSDESRLLLEVDEQGEPVSFISLARGMNGLQRGIGQAE
                     GVTMDTAGNIYIVSEPNLFYVLRKDPVAEQPAAAD"
     gene            17114..17461
                     /locus_tag="VFF22_08680"
     CDS             17114..17461
                     /locus_tag="VFF22_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019485285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HZX16919.1"
                     /translation="MKRFTLALLLVPLFSTAAMATGYTGPGATTQVTTVAAALDAADD
                     TPVVLQGQIVKRLQDELYEFKDASGTINVEIDDEHWPAQAISEKATVKISGEVDRDLT
                     SREIDVEYLELVK"
     gene            17550..>18367
                     /locus_tag="VFF22_08685"
     CDS             17550..>18367
                     /locus_tag="VFF22_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016263938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HZX16920.1"
                     /translation="MRDHFTLKRLLLSLVVLALLLLGLFAQDYRLFERAWFNVQQWQQ
                     VGGGQQDSMRLSDYQVDLEAKVIEGLNDDVSALTFDPDRNSLFTVTNQKPQLLELSLD
                     GELLRRIDLHGFGDAEAVEYISQGLYVISDERKHRLIKVRVNDATTELHADQAQQFSL
                     GIGLNGNKGFEGLAYDSAEQRLFVAKERDPMRIYEIHGFPQRDPQRPFAVHVVDDQKR
                     DAGLFVRDLSSLQFDERSGHLLALSDESRLVLELNVEGKPISSLSLLGGRNGLTR"
BASE COUNT         3206 a         5346 c         6036 g         3779 t
ORIGIN      
        1 ctgttctcgc agatagaaac taatttttga taggccgccc attcagtttt tttgtcgtct
       61 atttcaaaag cattaactat ttccagaact atttcgtgaa cagcttcgtg gagctctggg
      121 ttcatgctga aagcacctaa ctataattag acgacattcc caaatctgca cactattgcc
      181 actgctggca ataagtatcc acatctggct atgccgtatc catatgtgac catcagctat
      241 tgatgatccg ttcttgcagc cccaattgca ctgccgcttt gtagttcaac agccatgccc
      301 tctgttcagg ctgccacttg ccgccagcct gctttaccgc caattgcatg tccttctcgt
      361 gaaagccgat gcgcacgagt accaacgccg tcggttgtcc ggccggctgt gtgcgggttt
      421 tcggtggttt gtgtttgcgt tcgacgatca gctcgatggt ggtgtagctg atctgctttt
      481 cggcgtcgtg gcggtagcgc acggcgatca ggtcgtcgcc gtagcgttcg acaaagcgtt
      541 tggtgccgtt tttgcctggc ttgaggcttt ttacaacgtc catatcgtgc cccgctgagt
      601 ggcagtgtcc tgctggcagt ctgcctttat ccggctgggg gctcaagcag cgggcggagt
      661 aggacatcgc taactttcgg tttggtgagg gctcgctgct gcagccaagt aggcggtcag
      721 catgtgggtc agttcatcaa tcagttgttg gtcgctcagt ggcggttcgt ccagcgccag
      781 gtggcgcacc agggtcatca gggtggcgtt gatcatcaca aacagcatcg gcatggggtt
      841 gtcgatgggg aactggcgga tgtgacgcag cagataacgc cggcagactt ctgacaggta
      901 gcgttccagc cggtcaacca catgcagggc ctgcaactgc tgccaatggc gcaccagcac
      961 ccgataacgg ccattgccgc tgagaataaa tgcgagtacg tcgcttagca gcattctcac
     1021 cgccgttctg acgtcagcct ccagcagttg cgccatgcgc gcgtcgacca ggcgggtgat
     1081 atcgagcgcc atccgttcga gcagtgcttc cagcagttcc tgcttgtttg cgaagtactg
     1141 gtacagcgag cccacgctta cgcctgcctc gtcggcgact cggttggtgg tcaggttggc
     1201 caggccgtgg cgctcgagca cccggccgct ggcctctagc aggatgtcca ccatctgttg
     1261 tgagcgttgc tggcagggct ttttgcgcat gacggctcca ttgcgaattt aatgcgagct
     1321 aatactcgca ttagcatggg tgccaggcaa ttgcgcagga gcgccgaaca tgcagatgac
     1381 ttgtcccgtt tcagatacgc aaaccttaca gcctctaagc ccggactcgc tgacctggcg
     1441 ttatttcggt gatctgcgtg gcttgctgct gatcagtcgc gttggtctat tgcagaacct
     1501 gcatcctggc ctggctgctg gtgtgcagga acattctgat ctgttcgtta acccctggaa
     1561 ccgtctgttt cgctccattc cacccattct ggcggtggtc tatggcgctg aacgggcgcc
     1621 tgcaaccggt gtgcagattc gcgactggca ccggcagatc aagggcgtag atgctcaggg
     1681 gcggcgttac catgccctga gcccagagct tttctactgg gcccatgcca ccttttttga
     1741 agggcagatc gccgctcagg cgttgttcgg cacgccgctc gacgatgcgg cgctggagcg
     1801 tctatatcag gagtccatcc agtggtatgc cctgtacggt ctgccgatga cgacggttcc
     1861 ggccgactac aaggcgttca aggtttattg gcaggacatg ctggagtcac ggttggaaat
     1921 gacaggcgtg gcgcgctggt atctcgacgg caaccagcct ctgcccgccc cctatacctg
     1981 gatgcgtggg ccgctgtggt ggttgctgcg gccgttgttt gatcagggtg cacgttggtt
     2041 ggcgataggc agcttgcccg cgccattgcg tgagcggctg aagctgccct ggagcgcgaa
     2101 ggatcagcgg cgcctggacg gcctgtcacg gatggtgaga gcgctgtgga aagtgatgcc
     2161 ccggcgttgg cgatattttc ctcaggcaca cgccgctatg cgccgcgcgg gcaaagcagg
     2221 ttgatacggg caccggccga gcacgagaga cgttgcccct cgcgcggtta ccgtttaatc
     2281 ggccgggtta ttcaggctct gtagcagccg gtaatagaag ccgctggcgt tggctaatgt
     2341 atcgatccct acgcgttcat tggtgccgtg catgcgcggc aggtcttcgc gttccatcag
     2401 cagggggctg aagcggaaga tgttgtcggt cagcggctcg tagtagcgcg agtcggtgcc
     2461 gccgacggtg aggttggggc tgaccagggt gttgctaaag ctctgccgga tcacttcggc
     2521 gaactgtttg taggcagcgc cgttgacgtc ggaaaccgcc gagggctcgc ggccggcggg
     2581 catgactttg acctggatct gctcgtccgc cacggcgttg cgtacgtgtt cggccacgct
     2641 ttcgatgctg tcacgtgggt ggatgcgcag gttaagggtg gcgcgggcgc tgggcggcag
     2701 gacgttttcc ttgatgccgg cgcgcagcag ggtcggtgac agcgaagttt gcatttgcgc
     2761 ggcaccggct ggggaggcgg ccagctgctg ttccaccaat gggccgaata gccagaggtt
     2821 ggcgaatatc aaccgatagc cgaaggattg cgccggggtg aagctggcca gcaggtcgcc
     2881 ggtggggccg tcgaaaccgc gctggaaggg cgcatcgccc acccgctgca gggcctggct
     2941 caggcggccg atggctgttt catcgaaggc cggcggctgt gaggaatggc cgccctgggc
     3001 gttggcttgc agggtcaggc tgacgtagcc tttctccgca atgccgacca cggctacatc
     3061 ctggctgacg cctgggattt gcccgcgcac cacaaagccg ccttcatcgc tgaccaaggc
     3121 cagacgtttg ccttgctctt gcagttgcgc ggcgatgcgc cggttgccct ggtggccgcc
     3181 gatttcttca tcgtgtccaa aggccagcag cacatcgcgt tgcggctgaa agccctgggc
     3241 gatcagggtt tccaccgctt cgagaatcgc caccatcgaa cctttgctgt cgatggcgcc
     3301 gcgcccccag acaaagccat cggcaatcgc cccggagaac ggcggatggg tccacagttc
     3361 ttcggtgccg ggcgcgacag gcaccacgtc ctgatgcgcc atcagaaggg cggcaggtaa
     3421 ctggctgtct ttgcccggcc agcgcagcaa gatgctcggg ctgccggtgt ccagttgttc
     3481 ggtggtagca gtcaggcgcg ggtagcgttg cgccagccat tcgcgtaggc cggcaaatgc
     3541 cgcatcgctg gcagcctgtt ggctggccgg ggtgccgacc tgatgcgaca gggtcggcag
     3601 gcgaatcgcc gcagccaaac tctcggctgc acgttggcca tccagtcctt ctggcaggct
     3661 aaccggcgcg ctattgaagg tggcagccgg tagcagcaga gtgcggccga cgactacggc
     3721 aatcaggccg agcagagcga tcaataccac caatagcagg cgtttcattg cagtctcctg
     3781 ggcgcttagg gacgaagaat cagctggctg cggctattgg ccttgatcgc ctctgcatcc
     3841 aggtgcatcg gcacgtactc gccgcgcata taggcttcgg cctggtcctt gtagtgcttg
     3901 tcgaacagca cgccgctctg gccaaccgga ttgatgccca aggccgtgct ggagtcggcc
     3961 aggtcgatca ggcggcgggt cgaggggccg taaaccaccg accagggcgc agggcccacc
     4021 ggttgcgaca ggttgttcgg cgtttcatgg ccacccggcg cggcgaatgg gccgacgtta
     4081 aacagtttat ccagcggctt ctgctgaccc agcggatggc tgtgggtgag ggtatgcgcg
     4141 ctgccccagg tccattgagt ggcatcgtca ccctgggtgc tgcgcaggtg ggtgaggctg
     4201 gcctgccagg cagccttgac ggtatcggcg cggctttcca cggcatcgct gccgcgcttg
     4261 tcccaccagg gcgaactggc atcggcagcc aggcgcggca gggcgctgtc gagcacgcgg
     4321 gtttgcagca gattggtgaa gaaggcttcg cccagctcgt cggccatggc ctcaaacgtc
     4381 agctgataaa ccagctggtt gaacagcgtg gcggcagtgg cgtcgagagt atgttggcca
     4441 tcccagctca acagttgctc gaccagagct tgctcctcgg gatcacccac tgcggcacgc
     4501 aggtcggcgg ccagcggagc gagcaggcgt tgcgggtagc cggtgccggg gtcgagttgc
     4561 agcgcctgac tattggccag atcccacttc acgctgctgt cgctaagacg atcatccagg
     4621 cgctggccgc gatccgccag gttgtagtag ccgggtattt ccatgccggt cggcgagacg
     4681 ggctggtagt tggccgagac gatataaccg cgcgccgggt tctcttcctg cgggttggcg
     4741 ctgaacggga agtagccgtc cttttccgcc tcgccgctgg caccgtcgag gataaagctc
     4801 gggttgaccc cggccgggcg gcgcggcagc ttcgccgccg cccaccaacc gatatcgccc
     4861 gtggcattgg cccacaccac gttcagcccc ggtgcctcga tatgctcgac ggcggcgcga
     4921 gcctgctcca ggctggcggc gcggttgagt tggtagaaac catcgaggat cgggttctcg
     4981 gtttccagaa acgcccacca catggcaatc ggggtgctgc cgacggtttc acccagggcg
     5041 ctgttgatca gcgggccgtg gggcgagcgg cggatcgtca ggctgaccgg cgcggcgtcc
     5101 ttgaccgtga tctgctcaac gcggctttgc agctctaccc actggccgcg gtaccagact
     5161 tggttggggt tgtccgggtt ggttttctcg gcgatcaagt cgaggtcgtc gttctggaac
     5221 atggtcaggc tccaggcgaa ctcgcggtta tggcccagcg aagctaccgg gttgagcgcc
     5281 tggtgatggc cgtacaggct gaaaccgggg tagctcaggt tggcctcgta ccacaccgac
     5341 ggcacggcga agcggatatg cgggtcgccg gccagtagcg gtttaccgct ggcggtgcgg
     5401 ctgccggaga ctgcccaggc attgctgccc tcgaactgcg gcaggccggc gttgagcagc
     5461 gcggcgtggc ttagctcggc gatggcgttg agatcctgcc aatcagctgc ggcgaggttc
     5521 tgcccgacca cgccctgcgg gtgccagtcg aggtcgaagg ccttgaggta atcggcgccc
     5581 agctgatcgc ggatatgcgt caacactggt tcggtgcgaa atgccgcggc gaagctgtag
     5641 gccatataac cggcgacgct gagggtgtcg gcggtggtga acgggcgttt ctctataccc
     5701 aacacatcga actcgatggg tgccgggcgg ctggcctggt actggttgat gccgtccagg
     5761 taggcctgca gcgccttgct tgccggggtg ttcatgtcca tcttcgccgc gtagcggtcg
     5821 gcgtgttcgc gaatgcccag ggtgcggaac agacggtcag tgtcgaccag tttgctgccg
     5881 agaatctctg ccagctcgcc acgggccagg cgccgcagga tttccatctg gaacagccga
     5941 tcctgcgcat gcacatagcc cagcgcgcgg tacatatcgg cctcgttttg cgcctggata
     6001 tgcggcacgc cacgctcgtc gtaatccacc gtgaccggcg cctgcagtga actcaggctt
     6061 agctcgcccg cgcgttgcgg ctgcttgctg tcgatatacc agtaagcgcc ggcggcagtg
     6121 gctgcgacta gtacggcaag gacggtcagg gtgcgtttca tgggtgttcc ttgttatagg
     6181 ttttatcaac gcagccattg tgcgggtgga ttacggctcc ggcattgacc aaatacagca
     6241 ggagtgaaag tctttggtca attattgggg gctttatgga aaccgacccg cataacagca
     6301 tcgccagtgc gctgacccac tcctcgaccc tgcgccgcct gctctatgag gctttggcgg
     6361 agctcggcct ggacccgacc gacacttatc gccaggctta ccagggcgtg gtgctggcgc
     6421 cgccgctgct caatgcccgc gaggaccacg acaacgcgcc gcgcttctgg caggcgctgg
     6481 cgggcatcag tggtgatgtg gatatcggcc tgcacctggg cgaaaccatg cagccacggc
     6541 cgctggatgt ggtcggttac ctgctgctgg ccagtcgcga tctgggtcag gcgctggaga
     6601 gttttgtgcg ttttcagcac attctgtccg gtggctttgc cgcgcgtctg gagctggacg
     6661 acgagcaggt acggctgatc ttcgacctca actaccgtgg cgtcggctcg ctgcgccagc
     6721 agatggaatg tttggccctg ctgctgcaga agatgctcgc cagcatcaac aacgacggca
     6781 gctttcgcct gactgggctg gattttcgcc accctgcacc gcgccggctg agtgagcatc
     6841 ggcgtctgtt cggcctgctg ccgcgctttg cccaggcgca tgacgcgctg atcttcccgc
     6901 gcgcctggct tagccggcca tcgcgcagtg ccaacccgcg gttgtttgcg gtgctctggg
     6961 cgcaggcgga agtggagttg gctgagctgg aggaaaacca gttgctcaac cgcgtgcgct
     7021 actggctgga ggtcaacctg ggcgtacagc tgtgtgatct gccgcgctgc gccaaggccc
     7081 tcggatacag cgtcggcggg ctacagcggg cgttggccga acagcagcag agctttcgcc
     7141 agctgcacga tgaagtgcgc cgcctgcgcg cccaggccct gctcgacagc ggcctggcga
     7201 tccgcgaagt ggcgcgcgcc tgcggctttg ccgagctgtc gccgttctat cgcgcgttca
     7261 aacgctggca aggcgagacg ccgcagggct ttcgccgaca atcagacaaa tacggcggcg
     7321 gctgaacccg ctcagtcgtg cgcgacgtcg gcgctccagt cacaaaagca gccgaccgcc
     7381 agggtgttgc tggcgttgac cgggcgctct gcaataaggg catcgagaat cggctcgata
     7441 aagctgttgc ttgaggtgca ggtcgcgcct tcgctgtacg ggccgaagta ggccagttgg
     7501 ccgttgcggt cccagatcgc tacggccgga ctggcgggga tctgcgtgct acctggcagt
     7561 tcagtcagct gttgcatggc gctgagggtg gtgggcagtt gccctttgct gccaggtttt
     7621 tgtacggcgt aaaaggttac gccctggggg ccaaagcgtt cgatcagctc ggccaggtgc
     7681 tgttggttgc cgacattgca cgggcaggct ggatcccaga aatgcaccag gcggatcggc
     7741 ccagggccgg ccagctcatc cggcaggcgc agttgttcac cgtagaacag cgcggtctgc
     7801 tcgctgaagc tgcgcaggta gcgcgcttcg taccaccagt aggcggcgag catggcggcg
     7861 gcccaggcgg caatcagcag gctggctagt agggtcttgc gcgtgggtcg agtcatgggc
     7921 agccttcggc aaacaggcgc gcagcttgcc atgactggcg cgacagctga atatccgcaa
     7981 cctgattaca cgggccggga cggcccgtga ccaccccgga tgatcgatgg aagccctatg
     8041 ccagagccgt ttcagccaga aaatctgatt gccagtctgc gacccttggc cgcagccgcg
     8101 ccgatgtcgc tggctgagca gggctatcgg cagttctatg gctttagcga tggcttggcc
     8161 cggcacagtc gtctgggcgc ggtgcaggcc gctggctacc agattgcagt gcaggcctgg
     8221 tggccagagc agccgcgcgc gacctttgtg ctgctgcacg gttattacga ccacagcggc
     8281 ctgtatcgcc atgtgatcga gtgggcgctg ggcatgggct ttgccgtgct gagttttgat
     8341 ctgccgggcc atggcctgtc cagcggcgcg cgcgcaagca taggggattt tgccgagtac
     8401 cagacggtgc tgcaggcggt gctggttgaa gccgccgcat tagagctgcc gcagccatgg
     8461 cacctgtgcg ggcagagcac cggcggggcg attctgatcg actacctgct gaatggcgag
     8521 ccgctgacgc agattggcga aaccattctg ctggcgccgc tggtgcgacc acgggcttgg
     8581 ggctggtcgc agctcagcta tcgggtgatg cgcaactttg tcagcgagat tccccggcgt
     8641 ttcagcgtca attccagcga tgcagcgttt atcgacttcg tccatcacca ggatccgctg
     8701 cagccacgca atctaccgac cgcctgggtc ggcgcgctta gccaatgggt gccgcgcatc
     8761 gaggcggcgc cgcgcagtgc gcgcagcccg ctgatcattc agggcgaagc ggatatgacc
     8821 gtagcctggc agcacaacct tgaggtgttg cagaacaagt ttgccgcgcc gcagattttg
     8881 cgtctgcccg aggcgcgaca ccacttggcc aatgaagcgc cggccttgcg ccagcgctac
     8941 ttcgagtttc ttagtgagcg cctgagctaa tcagatgcct tgcggcgccg gagccaggct
     9001 ggcgctgctc tggccaactg ccaggccgct gcggatggcg gccagtgcag cctggtagta
     9061 gctcttgccg gtttctgact gagcaaagct cacaaattct tccagctccg gatcactcag
     9121 ctcgcggtag acgtgcagca aggtgttatc cagatccgca ccgatctgct ccatcaggcg
     9181 ctgacgctgg ccattgagca tggcctgggc gctgtcgcca ccgagcaagc cgggaagcat
     9241 ctgactaagg ctgtcggcgg ctacgccggc tagcgctagg ctgacttcgg caccggcctc
     9301 tttggctggc agggcctggg ccaagtggcg gatcagcagg cggcgggtgg cgctggcctc
     9361 actgcgcggc aggccgttgg cgtagcgcgc cagttgatcg ctgcgggtgg cgagcagttc
     9421 ggcgtcgaca atcttgcggc ccagtggcga ttggaagaat tgcagcgcgc tggtcgggtt
     9481 gtcgaggctg tcacgcaggc cctgttgggc gcgctgatcc accgcggcgg cggcgaagcg
     9541 ctggttgctg ttgttcacca gcgcctggtg taccgcaggc ggcaggctgc tgcggtagcg
     9601 ttcctgggcg gcctgcaggg catcgctgaa atgcatgcgc tgttgcggcc agccggccgc
     9661 ctgatacagc tgctggtaat cgtcggccag ggcgggcagg ctgagcagca gggtcagtac
     9721 aacggaaagg gcgcgcatag ggactccatt caggggcggc tattttctgc ggcatgtgga
     9781 cgcttgtcga ggctcggcag ggcgctggta gaatcgccgg catgacgact gatacccaca
     9841 ccgcctgcct gacccgcatc gttgacgacc tggccgaaca cggttggtcg ctgcagccgc
     9901 agtttattgc cccatctctg accctcgaac tggccgaaga gtgccgtaaa cgtgccgcgc
     9961 aaggtgcatt agcgcccgcc ggtgtcggcc gtggcgcgca gcagcagatt cgtgaagggg
    10021 tgcgcggcga ccatatccag tggctggaag ccggtcaggc cgaggtctgt gatcaatatt
    10081 tgcaggcgtt tgatgagctg cgcgtggcgc tgaaccaggg tttgtatttg gggctggagg
    10141 acttcgaggg gcatttcgcc ctgtatccgc cgggcgcgtt ctaccagaag catgtggatc
    10201 gctttcgcga tgatgatcgc cgcgcggtgt cggcagtgtt ctacctcaac gaaggttggc
    10261 aggccgagca gggcggtgcg ctacgtttgt atctgccgag cggcgagacc cgcgatgtat
    10321 tgccgcaagc gggcaacttg ctggtgtttc tctccgccga catgccccac gaagtgctgc
    10381 cagcgacgcg tgatcggttg tcgctgaccg gctggtttcg ccggcgtgga gatagtgtgt
    10441 tctagcgtac ttgagggcgc gagggctgga acctcaatgg ccccagatgc gcagttgccc
    10501 ctgcaggttg ctgaggtcgc ggatttccac ccggcccaga gtgctggagc tagacgcgcc
    10561 ggcatcgcca ataccgatgt cggcagcgat acgcatgcga atggtcagcg gcgtggtgat
    10621 caggccgtcg gccagcagct gatcactggt ggttttcaga tcgggcagtg ccagttgcat
    10681 cttctgcgtg ccgccctgat tgactatgtc gaccttcaga gtcggagcgt tgaccagggc
    10741 gctgttgaag tagagcttgg cggggccgta ggcgacgttg accggacagt cgccgggggc
    10801 ggcacagccg ttgtcgatct gcaggttgcg taactgcagg ctgccgccgt cgtcttccca
    10861 actgattttc gctgcctcgg ttttcaggtc cagggtcagg gtgatgccgg cctgaccggc
    10921 aatggcgctc agtgaggcgt tgtccagagc ctgcatttcc ccatgggcct gaccgagtgg
    10981 cagcaggcag atcagtaggg ccgacagcaa gggccgataa gttacggtgg gcatgatgcg
    11041 tatccctcgc tggaacagtg atggcacgca gtctggttgc gcgtgagctg tggcaccatt
    11101 gcccggcacg gcttcccgcc taatgatagg tcgggggctg tcgaggtcct ggactgcagt
    11161 gtgagcggct tggcagtttg accgccgtcc cgaccctttg gttagccggt aaaaggtggg
    11221 caccagcctg ggtgttacct agcgtgaccg tacagcggga gtgtgtgatg cagaaaatac
    11281 tggtaagccg ctgcctgctg ggtcatgccg tgcgctacga cggcggtgcc cacggcccct
    11341 ttaccgtgct tgagcgctgg cagcaggagg ggcgaattgt gccgttgtgt ccggaggtcg
    11401 ccggaggcct gtccaccccg cgcgcgccgg cggaaattgc cggcggccag ggtgcacagg
    11461 tactcgatgg acagctgccg gtgctgacgg tggacggcga ggatgtcact gccgctttta
    11521 ttgcgggtgc cgaacaggcc ttggcgctgg tggcgcagca tggcatccgc ctggcgctac
    11581 ttaaggcccg cagcccgtcg tgcggcaatc tggaggccta cgatggcacc ttcagcggcg
    11641 tgcgtgtgcc gggcgaaggg gtgaccgccg ccttgttgcg acgcgcaggt gtgcaagtgt
    11701 tcagcgagga gcaactcgag catgcgctgc aggcgctagt tgaactggag gtcagcgagg
    11761 gaacgtctgc aggtgtctag agtccacctc ttctctgccg cgtatcaagg aatcgacatg
    11821 aatagacgtc tgacttcatt tgctctgggt gctgcgctgc tttgcgggac ctttccctcg
    11881 gcctgggccg agatgccgcg cacagcagca ccggacggcg ctgccgtgta ttttatcgag
    11941 ccccaggacg gcgctacgct ggagcagacc ttcaccgtca agttcgggct caagggcatg
    12001 ggtgtagcac ctgccggcgt cgccgtgcag aacaccgggc accatcacct gctggtggat
    12061 gtgagcacgc tgccggccat gaacgcgcct ttgccgatga gtgatcaggt gcggcacttc
    12121 ggtgcgggtc agacggagac cgagctgagc ctggcgccgg gtactcacac cttgcagttg
    12181 ctgctgggtg atcagaacca tgtgccgcac gagccgccag tgctctcgga gaggatcacc
    12241 gtaacggtga agtaagcgtc cagcgccaaa agcggacata acgaaaaagg gaagccgaag
    12301 cttccctttt tcgtatcgcg gcagcgctta gaacagcacg cggctgcgga tggtcccgcg
    12361 tacatgctgc agtttttcca gtgccaagtc ggagtaatcg gcgtcgacgt cgattaccac
    12421 gtagccaacc ttgtcgttgg tctgcaggaa ctggccggag atgttgatgc cgttttcggc
    12481 aaacacgttg ttgatctcgc tcatcacgcc tgggatgttc tcgtggatgt gcagcaggcg
    12541 gtgcttgccc gggtgcgccg gcagggctac ttcggggaag ttgaccgagg acaccgaggt
    12601 accgttgtcg ctgtacttga ccagcttctc ggccacttcc aggccgatat tggcctgggc
    12661 ttcggcggtg gaaccaccga tatgcggagt caggatcacg cgatccaggc cgcgcagtgg
    12721 gctttcgaac tcttcgtcgt tggacttcgg ctcgaccggg aacacgtcga tggctgcacc
    12781 gatcagatgc tcgtccttga tcgcggcggc caggtgatcc agctcgacca cggtgccgcg
    12841 tgctgcgttg atcagaatgc cgcctttctt catggcgcgg atttccttct cgccgatcat
    12901 ccactgggtg gatggcagct caggcacgtg cagtgagacg atgtcgcaca tgcccagcag
    12961 gtcgtgcagc ttggctatct gggtggcgtt gcccagcggc agcttggtca cggtgtcgta
    13021 gaagaacacc tgcatgccca tggcttcggc gagtaccgag agctgggtgc cgattgagcc
    13081 gtaaccgacg atacccagct tcttgccacg gatttcgaag gagttggccg ccgatttgat
    13141 ccagccacca cggtggcagg aggcgttctt ctcggggatg ccgcgcagca gcaggatggc
    13201 ctgagccagc accagttcgg ccaccgagcg ggtgttcgaa tagggtgcgt tgaatacggc
    13261 gataccgcgc tcgcgggcag cgtcgaggtc aacctggttg gtgccgatgc agaagcaacc
    13321 gacggcgacc agacgcttgg cgcagtcgaa gacctcggcg gtcagctggg tacgcgagcg
    13381 gataccgata aagtgcgcgt cggcgatctt ttctttcagc tcgtcgtcgg agagtgcgcc
    13441 cttgaggtac tcgatgttgg tgtagccggc ggccttcagg gtgtccacgg cattctggtg
    13501 gacgccttca agaagaagga acttgatctt gctcttgtcg agagaggtct tgctgctcat
    13561 cggagtacct gttgtcccac aaatagtgtc agggaagggt cagcggcggg ggctgactgc
    13621 acccgcggat cggcgcgggg gcacgattca cggggggcgt atgctagcat acgagccccg
    13681 cgaagcacct accctggcag atgaagcgtg ctcagggtga ccatgaatcg tttgagagtt
    13741 ccgtagatga ccaaccctgc gctgatcgaa gagctgaaga ccctggttga gcccggcaag
    13801 gtgctcaccg atgccgattc cctcaacgcc tacggcaagg actggaccaa gcacttcgcc
    13861 ccagcgccgc tggcgattgc cttccccaag agcatcgagc aggtgcaggc gattgtccgt
    13921 tgggccaatc agcacaaggt cgcgctggtg ccgtccggtg gtcgtactgg cttgtccgct
    13981 ggtgccgtgg ccgccaatgg cgaagtggtc gtcgccttcg actatatgaa ccagattctc
    14041 gacttcaacg aattcgatcg caccgctgta tgtcagccgg gcgtggtgac caagcagctg
    14101 cagatgttcg ctgaggacaa gggcctgtat tacccggtgg acttcgcgtc ggccggctcc
    14161 agccagatcg gcggcaacat cggcaccaat gccggcggga tcaaggtgat tcgctacggc
    14221 atgacccgta actgggtggc cggtctcaag gtcgtcaccg gcaccggcga gctgctggag
    14281 ttgaacaagg acctgatcaa aaacgccacc ggttatgaca tgcgtcagct gtttatcggc
    14341 gccgaaggca ccctgggctt cgttgtcgag gccaccatgc gcctggaccg tgcgccgaag
    14401 aacctcaccg cgatggtgct tggcaccccg gatttcgatt cgatcatgcc ggtgctgcac
    14461 gccttccaga acaagctgga cctgaccgct ttcgaattct tctctgacaa agccctggcc
    14521 aagatcatgg cccgtggcga tgtgccagcg gcgtttgaaa cggattgccc gttctacgcc
    14581 ctgctggaat tcgaagccac caccgaggaa gtcgccgaag cggctctggc caccttcgag
    14641 cattgcgtcg agcagggctg ggtggtcgat ggcgtgatga gccagagcga gcagcagctg
    14701 cagaacctgt ggaagctgcg cgagtacatc tcggaaacca tcagccactg gacgccgtac
    14761 aagaacgaca tctcggtcac tgtgtcgaag gtgccagcgt ttttgcacga tatcgaccgc
    14821 attgtcggcg aacactaccc agacttcgaa atcgtctggt tcggccatat cggcgacggc
    14881 aacctgcacc tgaatatctt gaagccggaa agcctgtcca aggacgagtt cttcgccaag
    14941 tgcgccaccg tcaacaagtg ggtgttcgag accgtgcaga agtacaacgg ctcgatcagc
    15001 gccgagcatg gcgtgggcat gaccaagcgc gattacttga tctacagccg ctcggaagcc
    15061 gagattggct atatgaaggc gatcaaagcg gtgttcgatc cgaacgggat tatgaaccca
    15121 ggcaaaattt tcccgctctg acggcctggg cggctgttaa ttgcgccttt agtcgcaacg
    15181 acagtccgcg atgattcgcc cactatgggc gatcaattat tcagatcgta gggtggcatt
    15241 gcccgcccta cacagcctgc tcccacaaag agcggtgcaa cgcattccca taaggagttg
    15301 ctcatgagct accagcatca gtatgtcgat ggcacccata tccactttcc catgggcaag
    15361 gtggtgtgca tcggccgcaa ctacgccgag catgccaagg aactgaataa cccggtgccg
    15421 accgagccgt tgctgtttat caaggcgggc agttgtgtgg tgccgctgga tgacggcttt
    15481 accctggtcg agggtaaagg tgatgtgcat tacgaggcgg aaatcgccgt gctgatcggc
    15541 aagccgctgt cgcgcacgcc gagtgccgag gaagtacgcg atgccatctc cggcttcgcc
    15601 ccggctttgg acctgaccct gcgcgaccta caggccacgc tcaaggctaa gggttaccca
    15661 tgggaaactg ccaaatcctt cgacggtgcc tgcgtgctgg cgcctttcgt gccgggcgat
    15721 gcagtcgaag acctcgccga tatcggcatc cgtctggtga tcaacggcga agtgcgtcag
    15781 gacggcaata gccgcgacat gctcaacccg attctgccga tgatccagca catctgcggc
    15841 cacttcagcc tgcagccggg cgatgtgatc ctcaccggca cgccagtcgg cgtcggcccg
    15901 ctgcacaagg gcgatgagct ggtgctggag ctggttggtc atagccgctt cgagagtcgc
    15961 gcgctctaac cacgtttcga tccgtcaggc ttggttagtt ggcacctgga ggtgcccgct
    16021 ggcctggcgg cgtaacagaa gcttaagggt tgctctctag tctgatagcc atcgttcttg
    16081 tgagttgtgt gcgatgtcga tctggattgc tcggctggtg cgtctgcgct ggctaattgt
    16141 attgttcctc ctccttgtga ttgccctgct ggtgatgaat tatcgtcact gggatgaccg
    16201 cttttccttc tgggttcagc aacagcaggt ggatgctgcc gagcagcagg caagcatctg
    16261 gttgccgggc tataaggccg tgctgcaggg caaaccgctg cagggcctgg agggtgacga
    16321 ggtgtccggg ctgacctata gcccagagag cgacacgcta tttaccgtga ccggtaaaca
    16381 cccgcagctg attgagctga ccctggatgg ccaggtgatg cggcgcatcg atctgcatgg
    16441 cttttccaac cccgaggggg tggaaatgct tgccaatggt cgtctggcga ttatcgatga
    16501 gcgcaagcgc accctgacca ccttcaaact gggcgcgtta acccgcagcc tggagtttgc
    16561 cgatctggca tcgtttgatc tgggctttat cgatgctggc aacaagggct tcgagggcat
    16621 tgcctgggac tcgcgcaacg agcgcgtgct gctgggcaag gagcgtggcc cactggggct
    16681 gttcagccta cctttcccgg gcgaggacgg tgcggctgga gcgctccagc cgatgagttc
    16741 cgggcgcctg tttctgcgcg atatctcttc attgacctac gatcctcgaa ccgggcatgc
    16801 gctggtgctt tccgacgagt cgcgcctgct gctagaggtg gatgagcaag gtgagccggt
    16861 cagctttatc agcctggcca gaggtatgaa tggcctgcag cgtggtatcg gccaggccga
    16921 aggggtaacc atggataccg caggtaatat ttatatcgtc agcgagccca acctgttcta
    16981 cgtcctgcgc aaggatccgg tggccgaaca gcccgctgct gcggattaag gttggcttaa
    17041 gcgccgatta agcctggctt cagctacgcc ctctactctg gccccatcac ctaactgatt
    17101 agagggttac cccatgaagc gtttcacttt ggctctgctg ctggttccac tgttctccac
    17161 tgcggcgatg gccaccggtt acaccggccc aggcgccacc actcaggtca ccacggtagc
    17221 cgcggcgctg gatgcggccg atgacacccc ggtggtactg caaggacaga tcgtcaagcg
    17281 tctgcaggat gagctctatg agttcaaaga cgccagcggc accatcaatg tcgagatcga
    17341 cgatgagcat tggccagccc aggccatttc cgaaaaggcc acggtgaaga tcagcggtga
    17401 agttgaccgc gacctgacca gccgtgaaat cgatgtcgag tacctcgaac tggtcaagta
    17461 aaccgcccca tcatgtgccc acgccagccc gaagccctgc ttcgggctga cgtcgttgtg
    17521 ccacccgtga ctgttgagaa tgaagcgcca tgcgagatca tttcaccctg aagcgcctgt
    17581 tgctgagcct tgttgtgctg gcgttgttgt tgcttggtct gttcgctcag gattaccgtc
    17641 tgtttgagcg ggcctggttc aatgtgcagc aatggcagca ggtgggcggc gggcagcagg
    17701 actctatgcg gctgtcggat tatcaggtgg accttgaggc caaagtgatc gagggcctga
    17761 atgatgatgt ctcggcgctg accttcgacc cagaccgcaa cagcctgttc accgtgacca
    17821 accagaagcc gcagctgctg gagttatcgc tggatggtga gctgctacgg cgtatcgact
    17881 tgcacggttt tggcgatgct gaagcggtcg agtacatcag ccagggcctg tatgtgatca
    17941 gcgatgagcg caagcatcgt ctgatcaagg tgcgggtgaa tgacgccacc actgaactgc
    18001 atgccgatca ggcgcagcag ttctcgctgg gtatcggcct gaatggcaac aagggtttcg
    18061 aggggttggc ctatgactcg gccgagcagc gcctgtttgt cgccaaggag cgcgacccga
    18121 tgcgtatcta cgagattcac ggcttccctc agcgtgatcc gcagcgcccg ttcgccgtgc
    18181 atgtggtgga tgaccagaag cgcgatgccg gactgtttgt gcgcgacctg tccagcctgc
    18241 agttcgatga gcgcagcggc catctgctgg cgctgtccga tgagtcgcgc ctggtgctgg
    18301 agctgaatgt cgagggcaag ccgatcagca gcctgtcgct gctcggcggg cgtaatggcc
    18361 tgacccg
//
LOCUS       DASRUM010000129        32502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2233596, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000129 DASRUM010000000
VERSION     DASRUM010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 32502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32502
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2233596"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="VFF22_08690"
     CDS             complement(<1..1277)
                     /locus_tag="VFF22_08690"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HZX16921.1"
                     /translation="MPAFNKILIANRGEIACRVMRTAQDLGYRTVAVYSEADADARHV
                     QQADEAVCIGPAQVNQSYLLIANIIDAAQKTGADAIHPGYGFLSENADFARACEAAGI
                     VFIGPTVEAIHLMGSKRLSKIAMLEAGVPCIPGYEGAAQDDETLSREAGRIGYPLMIK
                     ASAGGGGRGMRLVHESGELLAQIRTARSEAQNAFGSGELILERAVIQPRHVEIQVFGD
                     QHGSIVYLGERDCSVQRRHQKVVEEAPCPVMTPELRQAMGEAAVKAAASVNYVGAGTV
                     EFLLDQSGEFFFLEMNTRLQVEHPVTELITGQDLVAWQIRVAEGQPLPLKQDEIRLTG
                     HAMEVRLYAEDATQNFLPQTGTALRWEPALREGIRIDHGLVEGQAITPFYDPMLAKVI
                     AYGATREEARRKLVRAVEDCVLLGVNGNQRFLA"
     gene            complement(1333..2133)
                     /locus_tag="VFF22_08695"
     CDS             complement(1333..2133)
                     /locus_tag="VFF22_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZX16922.1"
                     /translation="MAALPNCETLLLSLNAGVLHITLNRPESRNAMSLAMVTELRAVL
                     ESLRNDPSVRAIVLRGAGGHFCAGGDIKDMAGARAAGGDAYRSLNRAFGSLLEEAQSA
                     PQVLIAVLEGAVLGGGFGLTCVSDIAICQQDAKFGLPETTLGILPAQIAPFVVKRIGL
                     TQTRRLALTAARFNGSEAERLGLVHFSETDSEAVDARLHQVLSQVRQCAPQANAQTKA
                     LLLAAEYEALGPLLDRAAEQFAAAVTGGEGIEGTMAFMQKRAPKWAAE"
     gene            complement(2144..3304)
                     /gene="atuD"
                     /locus_tag="VFF22_08700"
     CDS             complement(2144..3304)
                     /gene="atuD"
                     /locus_tag="VFF22_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citronellyl-CoA dehydrogenase"
                     /protein_id="HZX16923.1"
                     /translation="MIFTQEHEELRRTVRNFVDKEINPHVEQWEKDGRFPIHDIFKKA
                     GDLGLLGISKPEKFGGMGLDYSYSIVAAEEFGTIICGGIPMSIGVQTDMCTPALARFG
                     SDELREEFLKPAIAGEMVGCIGVSEVGAGSDVAGLKTTAKKDGSDYVINGSKMWITNS
                     PSADFICLLANTSDDKPHVNKSLIMVPMNTPGITLSPHLDKLGMRSSETAQVFFDNVR
                     VPQRNRIGHEGAGFMMQMLQFQEERLFGAANMIKGLEHCINATIDYCKERKTFGNALI
                     DNQVIHFRMAELQTEIECLRALVYQATEQYVRGRDVTNLASMAKLKAGRLGREVTDSC
                     LQYWGGMGYMWENPVARAYRDVRLVSIGGGADEIMLGIICKLMGILPGKKKG"
     gene            complement(3323..4939)
                     /gene="atuC"
                     /locus_tag="VFF22_08705"
     CDS             complement(3323..4939)
                     /gene="atuC"
                     /locus_tag="VFF22_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranyl-CoA carboxylase subunit beta"
                     /protein_id="HZX16924.1"
                     /translation="MPVIQSEIDVHGEDFAKNREAMLAAVDSFRQIEQNCLNKAAEAK
                     AKFEKRGQLLPRERLNLLLDPGAPFLELSSLAGYKLHDDKDGTQAGGGIISGIGYIAG
                     VRCLVTASNSAIKGGTISPTGLKKTLRLQQIAFENKLPVVALTESGGANLNYAADIFV
                     EGARGFANQARMSAMGLPQITVVHGSSTAGGAYQPGLSDYVVVVRGKAKMFLAGPPLL
                     KAATGEIATDEQLGGAEMHAQVAGTAEYLAENDADGVRIAREILAMLPWNAQLPARPT
                     KAYSEPLYPVEELLGLVPADAKKPYDVREIIARIADGSAFLDFKNEFDNQTVCGHLQI
                     EGQPCGFIGNNGPITPQGAAKAAQFIQLCEQSNTPILFFHNTTGFMVGTESEQLGVIK
                     HGSKMIQAVANATVPKLTIVVGGSYGAGNYAMCGRGLDPRFIFAWPNSKTAVMGGAQA
                     GKVLRIVTEEKHLKEGKEADPKMLDMLETVTAQKLDSQSTALYGTASLWDDGLIDPRD
                     TRKLLGYLLDICAEAAVRPLKTTTFGVARL"
     gene            complement(4944..5813)
                     /locus_tag="VFF22_08710"
     CDS             complement(4944..5813)
                     /locus_tag="VFF22_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX16925.1"
                     /translation="MSYDSVFKPGLFIGQTIMVTGGGSGIGRCTAHELANLGAHVVLV
                     GRKLEKLEATCGEIIEDGGSASFQVCDIRDEDAVKAMVKTVVAERGQIHHLVNNAGGQ
                     FPAPLIGINQKGFETVVRTNLVGGFLIAKEVFLQCMSKHGGSIVNMLADMWGGMPGMG
                     HSGAARAGMENFTKTAAYEWGHAGVRVNAVAPGWIASSGMDTYPESMKNMIRSLKDHV
                     PLQRIGSESEVAAAIVFLLTPGANFISGNTIRIDGAASQGTRAWTLGKAKNSETYNGF
                     HRAYVPDVLKGEE"
     gene            complement(5816..7615)
                     /locus_tag="VFF22_08715"
     CDS             complement(5816..7615)
                     /locus_tag="VFF22_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HZX16926.1"
                     /translation="MTKTVRIGCASAFWGDTSTAAAQLVQGTELDYLVFDYLAEVTMS
                     IMAGARMKKPDAGYATDFVETLAPLLADIAAKNIRVISNAGGVNPSACAAALAAACEQ
                     AGVNLKIAVLHGDNLQPKFGELAKAGTVEMFSGAPLPPFCVSVNAYLGAPGIVEALKL
                     GADIVITGRVVDSAVVSAALVHEFGWSWSDYDQLAQAALAGHIIECGAQCTGGNFTDW
                     ESVPDYEHIGFPVVEVAADGSFVVSKPEGSGGLVTPFTVGEQMLYEIGDPRAYYLPDV
                     LCDFTQVQLAQVGDNRVELKGARGLPPTDQYKVSATHPDGFRCTASCLMAGIDAVKKA
                     QRVSQAIISKTEEMFVERGWGPYKEVSIELLGSEATYGPRGQRTDTREIVIKIAVRHA
                     KKEALVLFSREIAQAATGMAPGLTGIVGGRPTVYPVIRLFSFLADKDACAMEVELNGE
                     RTPVALPQITVLDSAQIAADIAAPLPSGQADACVPLIKLAVARSGDKGNHSNIGVMAR
                     KPDYLAWIAAALTKGAVAEWMQHALDPQTGKVSRWYLPGSHSLNFLLENALGGGGVAS
                     LRIDPQGKAFAQQLLEFPIPVPKALAASLEQGQ"
     gene            7858..8481
                     /locus_tag="VFF22_08720"
     CDS             7858..8481
                     /locus_tag="VFF22_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZX16927.1"
                     /translation="MDDQQAQAVMQQLVASGQITDPESARGKLLQTAAHLFRSKGYER
                     TTVRDLASAVGIQSGSIFHHFKSKDEILRSVMEETIRYNTALMHAALAEASDVRGRVL
                     ALIRCELQSIMGGTGEAMAVLVYEWRSLSEPGQAFILELRDNYEQLWLEVLSEAKAAG
                     YFKADPFILRRLLTGALSWTTTWFRPEGPMTLDQLAEEALSLVIKGA"
     gene            8628..9377
                     /locus_tag="VFF22_08725"
     CDS             8628..9377
                     /locus_tag="VFF22_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX16928.1"
                     /translation="MLSAVCVAQAAEEVRVGAYNFPPYVIKPESERPTGLLPELLQAL
                     NQLQSDYRFTLVATSTMRRYRDLPNGRFDLILFESPAWGWQDTAHVALDMHIEDAEVY
                     VARLQPGRDERYFDELKGKRLALYSGYHYGFAGFNSDKQFLTDTFNAMLTYSHDSNLI
                     MLLRGRADVAVVTRSYLRAYLQRYPEQSNALLVSQRVDQVYQHQALFRPESALQPQDL
                     AELLKQLKRDKQLDKLLGRYHLRDVSVLIND"
     gene            9482..10270
                     /locus_tag="VFF22_08730"
     CDS             9482..10270
                     /locus_tag="VFF22_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX16929.1"
                     /translation="MGVGLALLLTGPLLAAQDVRVAAVYFPPYVFKTEKSQANGLLDE
                     LVAALNQTQTDYRFVMVPTSVKRRFRDFEQQRIDLAIFENPGWGWQEIPHVALDMGLE
                     DSEVFVARTEPGREQGYFDSLEGKRLALYHGYHYAFAGFNSLPEYLSKTFSATLTHSH
                     ESNLLMVLHKRAEIALVTRSYIGDFLERNRQYAGQLLISDRVDQRYRHHVILSPEAPI
                     KPEQFMEIYEALRDNGQLEKIFSRYQVAVIPRAADSLKAAGAAN"
     gene            complement(10221..10979)
                     /locus_tag="VFF22_08735"
     CDS             complement(10221..10979)
                     /locus_tag="VFF22_08735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZX16930.1"
                     /translation="MPLHLRLMLPALCLATGFALGYIQLPGLAAGAALCALLLYADKY
                     LSNTLWTVLSLLACIAFAAHLVPGFSATPLWPPRQLSADAAPYALRLSWDKLLVGVTL
                     LAWWLGQKTPSKPLSTGVWLTAALTLLGVPLLALALGLVGWQPKWPAQLWLWLAINLG
                     VAVLAEELLFRGLLQSRLVNWLGTWAGIGLTALLFAAVHLPFSPLFAVVAGIAGLGYG
                     LIFHFSGRISLAIALHGAVNLLHLLLLSYPLRVG"
     gene            complement(11002..11169)
                     /locus_tag="VFF22_08740"
     CDS             complement(11002..11169)
                     /locus_tag="VFF22_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2897 family protein"
                     /protein_id="HZX16931.1"
                     /translation="MTWYIWLLILLVLGSIVGGLMVLLRTAKPLPLSEEQLEKIRQRE
                     LEQEAKDAREP"
     gene            11355..12659
                     /locus_tag="VFF22_08745"
     CDS             11355..12659
                     /locus_tag="VFF22_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX16932.1"
                     /translation="MTTASALSRLLKPVTPLAADMSIADVADRLLTPEHKAFLSLPIV
                     DSAGRPLGLVSRTTLQDIFMQRFGRDLRGRHAVQEVMNPAPLVVSLDTSLEDAAKQIT
                     GQLQYPITEDFILVDAQGLYRGLGTVLDLLKAMEARIAQRNLVLRKALVELKESQTQL
                     VQSEKMASLGQMVAGVAHELNTPLGYVKNNVQLLRELSAPLFELAEAQAALGDCLNDP
                     ACDEARLAMALEAAAEARAKAAPELLVEDFTQLFSDTLYGLEQIAELVVGLKDFARLD
                     RAMSEEVDLNECIRSALVIARYGIKDKAETILQLGELPRIPCAPSQINQVLLNLLNNA
                     AQAIDGFGQILVKTWADDSAVFVSVQDSGRGMPPEVLARIFDPFFTTKPVGQGTGLGL
                     SISYKIIQDHGGQIRAASEPGRGTRFLIRLPRPQALPLQRSA"
     gene            12661..13572
                     /locus_tag="VFF22_08750"
     CDS             12661..13572
                     /locus_tag="VFF22_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX16933.1"
                     /translation="MNEPIRILFVDDEERILRSLALQFRRDYQVLTESDPRRALERLK
                     SEPVHIIVSDQRMPQMSGAELLAEAQQIAPDTLRILLTGYSDLDAAVEALNSGGIFRY
                     LTKPWDQQEMAFTLRQAAQIAVRQLQTPKVSAAIPQAALNVLLLDEDAPTLDVVAEFC
                     VAGGHRLLRAASLNEAVALLNSQPVDLLVSDLHVAGEDTAPLLKSLAQAHPRLLSLVV
                     TPFCDTQALLKLINEAQIFRYLPKPIRPGLFDKGLQAAAEQAQKWRAQPAQVVTRLAQ
                     QPRDDREREKVGSLLGMLGRLRERLTA"
     gene            complement(13661..15007)
                     /locus_tag="VFF22_08755"
     CDS             complement(13661..15007)
                     /locus_tag="VFF22_08755"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HZX16934.1"
                     /translation="MSQPWSPESWRAKPIQQQPQYPDAVHLGKVEQTLAGYPPLVFAG
                     EARELRRQFAEVTQGRAFLLQGGDCAESFAEFSAAKIRDTFKVLLQMAIVMTFAAGCP
                     VVKVGRMAGQFAKPRSANDETIDGVTLPAYRGDIVNGIGFDSASRVPDPERLLQAYHQ
                     STASLNLLRAFAQGGFADLHQVHQWNLDFIANSALAEKYSHLANRIDETLAFMRACGM
                     DGSSQVRETSFFTAHEALLLNYEQAFVRRDSLTGGDYACSAHMLWIGDRTRQLDGAHV
                     EFLRGVGNPIGVKVGPSMDPDELIRLIDILNPDNDPGRLNLIVRMGADKVEAHMPRLV
                     RKVQGEGRQVLWSSDPMHGNTIKASTGYKTRDFAQILAEVKQFFAVHQAEGSYAGGIH
                     IEMTGQNVTECIGGARPVTEDSLSDRYHTHCDPRMNADQSLELAFLIAETLKQVRR"
     gene            complement(15106..15834)
                     /locus_tag="VFF22_08760"
     CDS             complement(15106..15834)
                     /locus_tag="VFF22_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZX16935.1"
                     /translation="MDIIWLTLASFILLAYTLEAITGFGSIVIALSLGALLLPIDQLL
                     PVLVPLNICMTGYLVWRHQQMIDRRLLLSMILPGMVVGTLIGYALLPFLDAVLAKRLF
                     GALVLWFAARELWRLRHGTAQPARPMWLSRLLSLCAGVSHGLFASGGPLLVFALAGTQ
                     LDKARLRATLVTVWFTLNSLLTLAFLLDGRLLPALPQVASYAPLLLIGVWLGERLHQR
                     FNEQHFRIAIYGLLLGTGALLVTA"
     gene            complement(15834..16616)
                     /locus_tag="VFF22_08765"
     CDS             complement(15834..16616)
                     /locus_tag="VFF22_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX16936.1"
                     /translation="MPTILITGAASGLGWQLARAYHDQGCNLLLTDINADGLSARVNE
                     LGAERVVGLAGDITDPHLHQQLLQACCERFGRLDVLINNAGITHRSPTLQTDPAVFRK
                     VMAVDYQAPVELTLSALPLLRQSRGQIIAIGSMAGWMPVLGRAGYCAAKSALGQFFEV
                     LRAEAARDGVKMLLVYPSFLDTPIEQNALGGDGKQAQHGRSTVGQIRGADWMAAQIIQ
                     AQEQGRERLFPDRGSWLASLLWRIAPGFYYRKMSARFAAEMG"
     gene            complement(16616..17551)
                     /locus_tag="VFF22_08770"
     CDS             complement(16616..17551)
                     /locus_tag="VFF22_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017516526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HZX16937.1"
                     /translation="MEPVNIQFDPSSLVLINVIVAVMMFGVSLELRLDDFKRIVREPK
                     APLIGLFAQFVLLPALTCLACWALRIEPMLALGMMLVAACPGGSFSNIMTWMARGNVA
                     VSVSMTAVSSLAASVLTPLNFGFYAWLNPLTRPLLTEIAMDPISLLLLVLLVLGLPLI
                     AGMWIGRRFPGLSLKVEKPLRIFALLVMLAFVTLAFSRNFDQFIAHFHLFFWLVVAHN
                     ALALAVGYGCARLSGLPAADRRAITLEVGIQNSALGLVIIFTFFPQASGMLLIAAFWG
                     CWHLVSGLTLAFIWSRRPPASLPLAPVALANAGEK"
     gene            complement(17551..18891)
                     /locus_tag="VFF22_08775"
     CDS             complement(17551..18891)
                     /locus_tag="VFF22_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZX16938.1"
                     /translation="MYAIIGAGPMGLCTARQLHKHGIDFVGFELHADVGGLWDIDNPH
                     STMYDSAHLISSKGTTQFSEFPMRAEVAPYPHHSEMRRYFRDYAEHFGLYAHYQFNTR
                     VVAIQRLDKGWKLISEQNGVQREWLVDGVLMANGTLHTPNQPALPGAFTGELMHSSDY
                     RSAAVFEGKRVLVIGCGNSACDIAVDAVHRAASVDLSVRRGYYFLPKFTLGRPTDTLG
                     GAIKLPRPLKQFLDGLLVRALVGKPSQYGLPDPDYKLYESHPVMNSLVLHYLGHGDIQ
                     ARRDVAKVDGLQVTFSDGEQAEYDLILQGTGYKLDYPLIDRAHLNWPSNAGAPQLYLN
                     VFHPEYDDLFMLGMVEASGLGWQGRDEQAELVALYIKGLQAGSQAAKDFQALKRERAS
                     MRLDGGYQYLQLERMAYYVHKDSYRQSIAQHSKSLRAGLAMLPATASRTVEGSL"
     gene            19055..19828
                     /locus_tag="VFF22_08780"
     CDS             19055..19828
                     /locus_tag="VFF22_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HZX16939.1"
                     /translation="MPGLPPVGQMPDPLLIAEVHDAFGVIQVVEMGAYRFLEFGEAIE
                     QSCVYMPDPSWLEYDYTRAMLLGALCHEHPESALFLGLGAGNLTQACLRFLPLEDVEV
                     IELRADVPRLAMEHMGLVDDPRLTIRIGDALELLESAEKTDLLFVDLYTDHGPATGHL
                     AWRFLENCQKQLNPGGWLIINQWAGNDGKPLGAPLLRGLYHRHYWECPVKEGNVVLLV
                     PADLDQQLDQDELLRRDELLAPQLGYSLRSLIQALRPAT"
     gene            complement(19929..20720)
                     /locus_tag="VFF22_08785"
     CDS             complement(19929..20720)
                     /locus_tag="VFF22_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZX16940.1"
                     /translation="MTQSTSGRVSREKRGQIMLIGLDRASKRNAFDMPMLDDMCLAYG
                     EFERDSEARVALVFAHGEHFTAGLDLANVAAQFAAGWKIPDGGCDPWGVFGGPRVSKP
                     VIVAAQGYCYTIGIELMLASDINLCASNTRFAQMEVQRGIFPFGGATLRMHQTAGWGN
                     AMRWLLTGDEFDAHEAYRLGFIQEVVASEELLPKALWLAERIAAQAPLGVQATLASAR
                     QAVVEGPTAAAASLHPTVTRLMASEDAKEGVKAMLERRPGDFKGR"
     gene            21053..22768
                     /locus_tag="VFF22_08790"
     CDS             21053..22768
                     /locus_tag="VFF22_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZX16941.1"
                     /translation="MGLCGCTRGRPMTQEIGGFAALDLHPNVLAAVIATGYEEPSAIQ
                     IQAIPVILAGHDMIGQAQTGTGKTAAFALPILSRIDPAKRQPQALILAPTRELALQVA
                     TAFETYAKQMPGVSVIAVYGGAPMGPQLKAIRNGAQIVVATPGRLVDHLRRDEKVLST
                     IQHLVLDEADEMLKLGFMDDLEVIFKAMPESRQSVLFSATLPHSIRSIAEKHLRDPKH
                     VKIETKTQTVTAIEQVHLMVHADQKHAAVLRLLEVEEFDALIGFVRTKQATLDLAAAL
                     EAKGYKAAALNGDIAQNQRERVIDSLKDGRLDIVIATDVAARGLDVPRITHVMNVDMP
                     YDPESYVHRIGRTGRAGRTGRALLLVTPRERRMLQVIERVTGQKVGEVKLPDAKQVLD
                     ARIKKLTNSLAPLVADAEASHGELLDRLTADIGCSPRALAAALLRKATNGQALTLAEV
                     ERDQPLVPNSSGPRERSERSDRPERPDRDGSRERRAPVPLAEGRARCRTALGARDGIA
                     AKNLLGAILNEGGLVREAIGRIQVRESFSLVELPEEGLERLLTKLKDTRVGGKPLKLR
                     RYRED"
     gene            complement(22919..23686)
                     /locus_tag="VFF22_08795"
     CDS             complement(22919..23686)
                     /locus_tag="VFF22_08795"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020299700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HZX16942.1"
                     /translation="MLPSLFISHGSPMLALQPGASGPALASLASQLPRPRAIVVVSAH
                     WESRELRVGSAEQPRTWHDFGGFPAELYQVQYPAPGNPTLAAEIIQLLAAANLPAQLD
                     AERPLDHGAWVPLTLMYPQADIPVVQVSLPSQHGPALQHQVGQALASLRAQGVLLIGS
                     GSITHNLGEVDWQAGPEVIEPWAQAFRDWIVAQLESQNTAALQDYRRQAPFAVRNHPS
                     DEHLLPLFFAEGAGGQFKVEHSGFTLGALGMDIYSFS"
     gene            23860..24516
                     /locus_tag="VFF22_08800"
     CDS             23860..24516
                     /locus_tag="VFF22_08800"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HZX16943.1"
                     /translation="MHAEFWQARWARSEIGFHLSEVNPYLQRCWPDLALAQGTRVLVP
                     LCGKSLDMLWLAGQGYRVCGVELAQKAVEDFFAEHQLQPVISEEGGFRVYRCEALEIY
                     CGDFFALTPQQVADCQGLYDRAALIALPPEMRARYVAHLAAILPPDCQGLLVTLDYEQ
                     AQMDGPPFAVPDSEVQQRLAGTWQVETLECCDVLGQNWRFLQRGLQRLDERVYRLRKQ
                     "
     gene            complement(24570..25442)
                     /gene="htpX"
                     /locus_tag="VFF22_08805"
     CDS             complement(24570..25442)
                     /gene="htpX"
                     /locus_tag="VFF22_08805"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HZX16944.1"
                     /translation="MMRIMLFLATNMAVLIIASITLKLLGVDRFTGQNYGSLLVFCAV
                     FGFAGSLISLFLSKWMAKMSTKTEIITQPRTRHEQWLLQTVEELSREAGIKMPEVGIF
                     PAYESNAFATGWNKNDALVAVSQGLLERFSPDEVRAVLAHEIGHVANGDMVTLALIQG
                     VVNTFVMFFARIFGNFVDKVILKNEDGPGIGYFVATIFAELVLGILASIIVMWFSRKR
                     EFKADEAGARLAGTGAMIAALQRLRAEQEVPVTMPNSLTAFGINGGLKHGLAGLLMTH
                     PPLEDRIEALRQRG"
     gene            complement(25587..26039)
                     /locus_tag="VFF22_08810"
     CDS             complement(25587..26039)
                     /locus_tag="VFF22_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16945.1"
                     /translation="MDLQIDEFYKDAAAGLLSLYQVFPRKAALYVEDLIGREEPDEFG
                     LPTPRHQSCLGALLWLAEEGYIRFDSTIGYDALDQAVLSEKGFLRLTRAVPHALREGE
                     ALPPSVRRVHATLAFQLREALGQRHGERISRLSRLLFESSLNGASDIV"
     gene            complement(26039..26782)
                     /locus_tag="VFF22_08815"
     CDS             complement(26039..26782)
                     /locus_tag="VFF22_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZX16946.1"
                     /translation="MSQGKRYLLTGASSGIGAALARELAAKGYDLALAARREDSLHLL
                     AAELQARFKRKVVVLPLDVTDYDACQDAVGLAANLLGGLDGIIANAGIALTGKAGGGH
                     FKRARLTVETNLIGAIACLDAACALFRQQGHGHLVAVASVAGKRGLPHTAAYSASKAG
                     LISYMEATRAELLGKNIDTTLLLPGFIDTPLNSDMANRPFLIDVERGARLIARHIDKR
                     AASAYVPGWPWALLGRLLPYLPTSLIGKF"
     gene            complement(26795..28006)
                     /locus_tag="VFF22_08820"
     CDS             complement(26795..28006)
                     /locus_tag="VFF22_08820"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZX16947.1"
                     /translation="MQFSKSNKLANVCYDIRGPVLKHAKRLEEEGHRILKLNIGNPAP
                     FGFEAPEEILQDVIRNLPTAQGYSDSKGLFSARKAVMQYYQQKQVEGVGIEDIYLGNG
                     VSELIVMAMQALLNNGDEVLIPAPDYPLWTAAVALAGGKPVHYLCDEQAGWFPDIADM
                     KAKITPNTKALVLINPNNPTGAVYSKEVLLDIVELARQHNLVLFSDEIYDKILYDEAQ
                     HICTASLAPDVLCLTFNGLSKSYRVAGFRSGWVAISGPKHRAQSYIEGIDILANMRLC
                     ANVPSQHAIQTALGGYQSINDLVLPNGRLLEQRNRTWELLNDIPGVSCVKPMGALYAF
                     PKIDPKVCPIHNDEKFVLDLLLSEKLLIVQGTAFNWPWPDHFRVVTLPRVDELEMAIG
                     RIGSFLKTYQQ"
     gene            28177..28572
                     /gene="msrB"
                     /locus_tag="VFF22_08825"
     CDS             28177..28572
                     /gene="msrB"
                     /locus_tag="VFF22_08825"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZX16948.1"
                     /translation="MDKLEKPLEAWREELSEEQFHVCRLGGTERAFTGQYHDSKVPGI
                     YHCACCQAELFDSDAKYDSGSGWPSYFQPVSSEAIVSLDDFNHGMHRIEVKCAKCDAH
                     LGHVFPDGPAPTGLRYCINSASLKLVPKA"
     gene            28682..29167
                     /locus_tag="VFF22_08830"
     CDS             28682..29167
                     /locus_tag="VFF22_08830"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HZX16949.1"
                     /translation="MSNALFDIPVTTIKGEQKTLADFGGKAVLVVNTASKCGFTPQYK
                     GLENVWQQYKDKGLVVLGFPCNQFGKQEPGDEGAISEFCELNFGVSFPLFKKVDVNGS
                     DAHPLFVNLKKSAPGLLGSQGIKWNFTKFLVSADGLVIKRFAPTTKPEDLTAEIEALL
                     K"
     gene            29164..29637
                     /locus_tag="VFF22_08835"
     CDS             29164..29637
                     /locus_tag="VFF22_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015636529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZX16950.1"
                     /translation="MTQTTLHADDGLQLDNQLCFKLYAASRAVIRAYKPMLDQLGLTY
                     PQYLAMLVLWEWQQQPPALPTVKALGERLLLDSGTLTPLLKRLELQGLLQRKRSASDE
                     REVHLALTPAGCALREQVLPLKSQLLCERGIDLDQVTELRSQVGVLLDQFMVLNH"
     gene            complement(29613..31925)
                     /locus_tag="VFF22_08840"
     CDS             complement(29613..31925)
                     /locus_tag="VFF22_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011062517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX16951.1"
                     /translation="MDIALTQRLSFKQAGLTVLVAFILGTALSLIQVGIDYASEDASI
                     NREINALMEISHNPAARIAYNIDAELAQELVLGLLRSPAVISAQIIDNNQLTLARVSR
                     PAMESRYRVLSDFMFGKRRHFETALFVSHSPSEALGKLSLEVDTYAFGSHFLNRSLLT
                     LLTGFARSLLLSLILLVLFYFMLTKPLTGFIRALSERDLRSPNNAKLPYPPNHERDEI
                     GVLVDVTNRQLSSIASEIEQRQQAEARLTRYLAELEDMVSARTEQLEAANSRLLDSNR
                     ELEQAKRTALDMAQARSAFLANMSHEIRTPLNGLLGMLALSLDGPLNNEQRQQLSIAH
                     DSGKVLVELLNDILDLSKFEAGQLELEQIPFDLASLVEDTANLLSQNAAPGVELTCLI
                     DPQLPNQLLGDPTRVRQIVSNLLSNALKFTRFGRVDISVSAQTGGVRIIVRDTGIGIA
                     EDAQTRIFHPFAQAGVGITRQFGGTGLGLALTRRLCEAMHGQLSLETREGFGSTFCID
                     LPLLGQANAQQRPVLKGRILGLSPASSGLSEQLASLLPSWGLQYQHLDADAALGQLEA
                     DLLISDCPQRLSELRTLCKLPILLVSAYGNFLSSEQVEKLSPIEQLARPLSRQALLQA
                     LRRTLGQGEAHAAEQHSEPQRNRQARVLLVEDNPVNQLVAKGMLGKLGCEVLLANHGG
                     EALSLLETQVVDLILMDCNMPVMDGYETTQRIRQKTKFAELPIIALTANALSDERERC
                     RAAGMDDYLAKPFRKDELAALLDQWLSTIN"
     gene            32025..>32502
                     /locus_tag="VFF22_08845"
     CDS             32025..>32502
                     /locus_tag="VFF22_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HZX16952.1"
                     /translation="MDSRLSDFLLRAEAVMARLEPLLPIQRPVIDWQQSLAARWQRDG
                     RSGFLAPLQVSLDVNLDDLIGVDRQRAQLARNTQQFIQGLPANHALLWGARGTGKSSL
                     VRALLAEHAAAGLRLIEIERDHLADLPRIVEQLSELPQRFVLFCDDLSFEAGEGDYR"
BASE COUNT         5993 a         9554 c        10467 g         6488 t
ORIGIN      
        1 ttggcgagaa agcgctggtt gccgttgacg cccagcagca cgcaatcctc aaccgcacgc
       61 accagcttgc gccgcgcttc ttcgcgggtg gcgccgtagg cgatgacttt ggccagcatc
      121 gggtcgtaga acggcgtgat cgcttggcct tcgaccaggc cgtggtcgat gcgaatgccc
      181 tcgcgcagcg ccggctccca gcgcagggcg gtgccggttt gcggcaggaa gttctgcgtg
      241 gcgtcttcgg cgtacaggcg cacttccatg gcgtggccgg tgaggcgaat ctcgtcctgc
      301 ttaagcggta gcggctggcc ttcggcgacg cgaatctgcc aggcgaccag gtcctggccg
      361 gtgatcagtt cggtgaccgg atgctccacc tgtagacggg tgttcatttc cagaaagaag
      421 aattcgccgc tctggtccag cagaaattcc acggtgcccg cgccgacgta gttgaccgac
      481 gcggccgctt tcactgccgc ctcgcccatg gcctgacgca gctcaggcgt cattaccggg
      541 cagggtgctt cctcgaccac cttctggtgg cggcgttgca ccgagcagtc gcgctcgccg
      601 agataaacga tgctgccgtg ctggtcgccg aacacctgaa tttccacatg gcgcggttgg
      661 atcactgcac gttcgagaat cagctcgccg gagccgaagg cgttctgcgc ctcggagcgg
      721 gcggtgcgga tctgcgctag cagctcgccg gattcatgga ccaggcgcat accacggccg
      781 ccgccgccag cactggcctt gatcatcagt gggtaaccga tacgcccggc ctcgcggctc
      841 agggtctcgt cgtcctgggc tgcaccttcg tagccgggaa tgcacggtac gccggcttcg
      901 agcatggcga tctttgacag gcgcttgctg cccatcaggt ggatggcttc cacggtcggg
      961 ccaataaaca cgatgccggc ggcctcgcag gcgcgggcga agtcggcgtt ttccgagaga
     1021 aagccgtagc cgggatggat cgcatcggcc ccggttttct gcgccgcgtc gatgatattg
     1081 gcgatcagca gataggactg gttgacctgg gccgggccaa tgcacacggc ttcatcggct
     1141 tgctgcacat ggcgtgcgtc ggcgtcggcc tccgagtaca cggccacggt gcggtagccg
     1201 aggtcttggg cggtgcgcat cacccggcag gcgatttccc cacggttggc gatcagaatc
     1261 ttgttaaagg cgggcatctg tgtgctcctg ctggggtcgt aagcctggca atcaggtcat
     1321 ttgtagaggg gcttattcag cggcccactt cggcgcacgt ttctgcataa aggccatggt
     1381 gccctcgatg ccttcgccgc cagttaccgc cgcggcaaac tgctcggctg cgcggtccag
     1441 caacggcccg agtgcttcgt attcggcggc cagcagcagg gccttggtct gcgcattggc
     1501 ctggggcgcg cattggcgca cctggctaag cacctggtgc aaacgggcgt caacggcttc
     1561 gctatcggtt tcgctgaaat gcaccagacc caggcgttcg gcttcgctac cgttaaagcg
     1621 ggcggcggta agggccaggc gacgggtctg ggtcaggccg atgcgcttga ccacaaaggg
     1681 tgcaatctgc gctggcagaa tccccaaggt ggtttccggc aggccgaact tggcgtcctg
     1741 ctggcagatg gcgatatccg acacgcaggt caggccaaaa ccgccgccca gcaccgcacc
     1801 ttcgagcacg gcgatcagca cctgtggcgc gctctgcgct tcttccagca ggctgccgaa
     1861 ggcgcggtta aggctgcggt aggcatcgcc accggccgca cgagcgccgg ccatatcctt
     1921 gatatcgccg ccggcgcaga aatgcccacc ggcaccgcgc aggacaatgg cgcgcacgct
     1981 tgggtcattg cgcaggcttt ccagcaccgc gcgcagctcc gtgaccatgg ccaggctcat
     2041 ggcgttgcgg ctttccgggc gattaagggt gatgtgcagc acgccggcgt tgaggcttag
     2101 cagcagggtt tcgcagtttg gtagagcggc catggtcgcg tcctcagcct ttctttttgc
     2161 caggcagaat gcccatcagc ttgcagataa tgcccagcat gatttcgtcg gcaccgccgc
     2221 cgatgctcac caggcgcacg tcgcggtagg cgcgggccac cgggttttcc cacatgtagc
     2281 ccatgccgcc ccagtactgc aggcagctgt cggtgacttc acggccgagg cggccggcct
     2341 tgagcttggc catcgaggcc aggttggtga cgtcgcggcc gcgcacgtat tgctcagtgg
     2401 cctggtagac caaggcgcgc aggcattcga tttctgtctg caactcggcc atgcggaagt
     2461 ggatgacctg gttgtcgatc agcgcattgc cgaaagtttt gcgctctttg cagtagtcga
     2521 tggtggcgtt gatacagtgc tccaggccct tgatcatgtt ggcagcgccg aacaggcgct
     2581 cttcttggaa ctgcagcatc tgcatcataa agcctgcgcc ttcgtggccg atgcgattgc
     2641 gctgcggcac gcgtacgttg tcgaagaaca cctgagcggt ctccgaactg cgcatgccga
     2701 gtttgtccag gtgcgggcta agggtgatgc ctggggtgtt catcggcacc atgatcagcg
     2761 acttgttgac gtgcggcttg tcgtcgctgg tattggccaa caggcagata aagtcagcgg
     2821 atggggaatt ggtaatccac atcttgctgc cgttgatcac gtagtcgctg ccgtctttct
     2881 tggcggtagt tttcagcccg gcgacatccg agccggcgcc gacttcggaa acgccgatac
     2941 agccgaccat ctcgccggca attgccggtt tgaggaattc ttcgcgcagc tcatcggagc
     3001 cgaagcgcgc caacgccggg gtacacatat cggtttgtac gccgatggac atcgggatgc
     3061 cgccgcagat gatggtgccg aactcttcgg cggcgacgat cgagtagctg tagtccaggc
     3121 ccatgccgcc gaatttttcc ggcttggaga tgcccagcag gccgaggtcg ccggctttct
     3181 tgaaaatatc gtggatcggg aagcggccgt ccttttccca ctgttcgacg tgcgggttaa
     3241 tttccttgtc gacaaaatta cggaccgtgc ggcgcagctc ttcgtgttct tgggtaaaga
     3301 tcatttttta ttctccgaag ggttacaggc gggctacgcc gaaagtagtg gttttaagcg
     3361 ggcgaacagc ggcttcggca cagatatcca gcagatagcc gagcagcttg cgcgtatcgc
     3421 gcgggtcgat caggccgtcg tcccacaggc tggcggtgcc atagagcgcg gtggattggc
     3481 tatcgagttt ctgcgcggtg acggtttcca gcatgtcgag catcttcggg tcggcttctt
     3541 tgccttcctt caggtgtttt tcctcggtga cgatgcgcag caccttgccg gcctgggcgc
     3601 cacccatcac ggcggttttg ctgttgggcc aggcgaagat aaagcgcggg tcgaggccgc
     3661 ggccgcacat ggcgtagtta ccggcgccgt aggaaccgcc gacgacgatg gtgagtttcg
     3721 gcaccgtggc gttggccacc gcctggatca tcttcgagcc gtgcttgatc accccgagtt
     3781 gttccgactc ggtgccgacc ataaagccgg tggtgttgtg gaaaaacagg atcggcgtat
     3841 tgctctgctc acacagttga atgaactgtg ccgctttggc cgcgccttgc ggggtgatcg
     3901 ggccgttgtt gccgataaag ccgcagggct ggccttcgat ctgcaaatgg ccgcagacgg
     3961 tctggttatc gaactcgttc ttgaagtcga ggaaggccga gccgtcggcg atacgagcaa
     4021 tgatctcgcg cacgtcgtag ggcttcttgg catctgccgg cactaggccg agcagttctt
     4081 cgaccgggta cagcggctcg ctgtaggcct tggtcgggcg cgccggtagt tgcgcgttcc
     4141 acggcagcat ggcgagaatt tcccgggcga tacgcacacc atcggcgtca ttctcagcca
     4201 ggtattcggc ggtgccggcg acctgggcgt gcatctcggc accgcccagt tgctcatcgg
     4261 tggcgatttc gccggtggcg gctttcagca gcggcgggcc ggcgaggaac atcttggcct
     4321 tgccgcgcac caccaccaca taatccgaca gtcccggctg gtaagccccg ccagccgtag
     4381 acgagccatg caccaccgtg atttgcggca ggcccatggc cgacatgcgc gcctggttgg
     4441 caaagccgcg cgcgccttcg acaaagatat cggcggcgta gttgaggttg gcaccgccgc
     4501 tttcggtcag cgccaccacc ggcagtttgt tctcgaaggc gatctgctgc aggcgcaggg
     4561 tttttttcag cccagtaggg gagatggtgc cgcccttgat tgcgctgttg ctggcagtga
     4621 ccaggcagcg cactccggct atatagccga taccggaaat aatcccaccg ccggcctggg
     4681 tgccgtcctt gtcgtcgtgc agcttgtagc cggccagcga tgacagttcg agaaagggcg
     4741 cgccggggtc gagcagcagg ttaaggcgct cacgcggcag caactggccg cgcttttcga
     4801 atttggcttt ggcctcggca gccttgttca ggcagttctg ttcgatctgg cgaaagctgt
     4861 cgacggcggc gagcatggcc tcgcggttct tggcgaagtc ctcgccatgc acatcgatct
     4921 cggattggat aaccggcatg gcgttactcc tcacccttga gcacgtcagg cacgtaggcc
     4981 cggtggaagc cgttataggt ctcgctgttt ttcgccttgc ccagggtcca ggcgcgggtg
     5041 ccctggctgg cggcaccgtc gatgcggatg gtattgccgc tgataaagtt ggcccccggc
     5101 gtgagcagga agacgatggc ggcggccact tccgattcgc tgccaatgcg ttgcagcggc
     5161 acgtgatcct tgagcgagcg gatcatgttc ttcatcgatt cggggtaggt gtccatgccg
     5221 ctggaggcga tccagcccgg tgccacggcg ttgacccgca ctccggcgtg gccccattcg
     5281 taggcggcgg tcttggtgaa gttctccatg ccggcgcgcg ccgcaccgga gtggcccatg
     5341 ccgggcatgc cgccccacat atcggcgagc atattgacga tgctgccgcc atgcttgctc
     5401 atacactgca ggaacacttc cttggcgatc agaaagccgc ccaccaggtt ggtgcgcacc
     5461 acggtctcga agcctttctg gttgatgccg atcagcggtg cggggaactg accaccggcg
     5521 ttgttgacca ggtgatggat ctgcccgcgc tcggcgacta cggttttgac catggccttg
     5581 accgcgtctt catcacggat atcgcagacc tgaaagctgg cgctgccgcc atcttcaata
     5641 atttcgccgc aggtggcttc gagcttttcc agcttgcgtc cgaccagcac cacatgggca
     5701 ccgaggttcg ccagctcatg ggcggtgcaa cggccgatgc cgctgccgcc gccggtgacc
     5761 atgatggtct ggccgataaa gaggccgggt ttaaacaccg agtcgtagct cattgttatt
     5821 gtccttgttc gaggctagcg gccagcgcct tgggcaccgg tatcgggaat tccagcaact
     5881 gttgggcgaa ggccttgcct tgcgggtcga tgcgcaggga ggcgacgccg ccgccgccca
     5941 gggcgttctc cagcaggaag ttcaggctat ggctgccggg cagataccag cggctgacct
     6001 tgccggtttg cgggtcgagc gcgtgctgca tccattcggc cacggctccc ttggttagcg
     6061 cagcggcgat ccaggccaag taatcgggct ttctggccat tacaccaata ttgctgtggt
     6121 tgcccttgtc gccagagcgc gctacggcca acttgatcag aggtacgcag gcgtcggcct
     6181 ggccgcttgg tagcggggcg gctatatcgg cagcaatctg cgcgctgtcc agcaccgtga
     6241 tctgcggcag tgcgacaggc gtgcgttcgc cgttcagctc cacttccatg gcgcaggcgt
     6301 ctttgtcagc gagaaagctg aacaggcgga tcaccgggta aaccgtgggg cgaccgccga
     6361 cgatgccggt cagtcccggt gccattccag tcgctgcctg ggcaatctca cgggagaaca
     6421 gcaccagggc ttcttttttg gcatggcgca cggcgatctt gatcacgatc tcgcgggtgt
     6481 cggtgcgctg gccacgcggg ccgtaggtgg cttcggaacc cagcagctcg atgctgactt
     6541 ccttgtaagg cccccagccg cgctcgacga acatttcttc ggtcttgctg atgatcgcct
     6601 ggctgacccg ctgggctttc ttcaccgcat ctatgcccgc catcaggcag ctggcagtgc
     6661 agcggaaacc gtccgggtgg gtggcgctga ccttgtattg gtcggtgggc ggcagaccgc
     6721 gtgcgccttt gagttcaacc cggttatcgc caacctgggc cagttgcacc tgggtgaaat
     6781 cgcagagtac atcgggcagg taataggccc gtggatcgcc gatttcataa agcatctgct
     6841 cgcccacggt aaaaggcgta accagaccgc cggaaccttc cggtttgctc acgacaaagc
     6901 tgccatcggc tgccacttcc accaccggga agccaatgtg ttcgtagtcc ggcacgcttt
     6961 cccagtcggt gaagttgccg ccggtgcact gcgcgccgca ttcgatgata tgtccggcca
     7021 atgcggcttg ggccaactgg tcatagtcgc tccagctcca gccaaattca tgcaccaggg
     7081 ccgcgctgac cacggcgctg tcgactacac ggccggtgat gacgatatcc gcgccgagct
     7141 tgagtgcttc gacaatgccc ggcgcgccga ggtaggcgtt gaccgacacg cagaagggcg
     7201 gcaacggcgc gccgctaaac atttccacgg tgccggcctt ggccagctcg ccgaacttgg
     7261 gctgcaggtt gtcgccgtgc agcacggcaa tcttcaagtt aacccctgct tgctcgcaag
     7321 cggctgccaa agcggcggcg caggcgctgg ggttgacccc accggcattg ctgattacgc
     7381 ggatgttctt ggcggcgata tcagccagca gcggcgcgag ggtttccaca aagtcggtgg
     7441 cgtagccggc atccggcttc ttcatccgtg cgccggccat gatcgacata gtcacttcgg
     7501 ccaggtagtc gaataccaga taatccagct cggtgccctg taccagctgg gcggcggcgg
     7561 tgctggtgtc gccccagaag gcggaggcgc agccgatgcg tacggtttta gtcattggaa
     7621 taggtccgca acacataatt aaggtgaatc gaaggctacc aagcaagcgc ttggttgaaa
     7681 agccccaagg agaatctttc taaccaaaag tgtggccaag cgcttgcttg ggtggctggc
     7741 gcagcataaa tttcaccaat aacgacaagc acttgggtaa acccgctgag aatcagcgtg
     7801 caatttcacg ctgggccagt tagcccaagg cataaaaaga gttggagagt acttagcgtg
     7861 gatgatcaac aagctcaggc ggtaatgcag caacttgtgg ccagtgggca gatcaccgac
     7921 ccggagagtg ctcgtggcaa gctactgcaa accgccgcgc atctgtttcg cagcaagggc
     7981 tacgagcgca ccacagtgcg cgacttggcc agtgctgtgg gcattcagtc cggcagcatc
     8041 tttcatcact tcaagagcaa ggacgagatc ctgcgctcgg tgatggagga gaccattcgc
     8101 tacaacactg cgctgatgca tgccgccctg gccgaggcga gcgacgtgcg tggtcgcgtg
     8161 ttggcactga ttcgctgcga actgcagtcg attatgggcg gtaccggcga ggccatggcg
     8221 gtactggtgt atgaatggcg ttcgctgtct gagccgggcc aggcgtttat tctcgaactg
     8281 cgcgacaact acgagcagct ctggctggag gttctgagcg aagccaaggc ggccggctac
     8341 tttaaggccg acccgtttat ccttcgccgc ttgctgactg gcgcgctgag ctggaccact
     8401 acctggttcc gcccagaagg gccgatgacc ctcgaccaac tggccgaaga agcgctgtcg
     8461 ctggtgatca agggcgcttg aggaaattca gctaagctga gatggcatat tgatattgtt
     8521 ttggcgccaa aggattgcga gcctttcagg ttacggaaga agtcgctgcg ttttgttctg
     8581 aggttcgccg cttggccacg ctgttacggg ggttactgct cagtttgatg ctttctgcag
     8641 tgtgcgttgc gcaggctgcg gaagaggtgc gagtgggtgc ctacaatttc ccaccctatg
     8701 tgattaaacc ggaaagcgag cgtcctactg gtctgctgcc ggagttgttg caggcgctta
     8761 atcagctgca aagcgactac cgctttaccc tggttgccac ctcgacgatg cgccgctacc
     8821 gcgatctgcc aaacggccgc ttcgacctga tcctgtttga atccccggca tggggttggc
     8881 aggacaccgc acatgttgcg ctggatatgc atatcgaaga tgccgaagtc tatgtcgcac
     8941 gcctgcagcc ggggcgtgac gagcgctatt tcgatgaact caagggcaag cgcctagccc
     9001 tgtacagcgg ttatcactac ggatttgccg gatttaactc ggacaagcag tttctcaccg
     9061 atacattcaa cgcgatgctg acctactctc acgacagcaa cctgatcatg ctgctgcgcg
     9121 ggcgggccga tgtggcggtg gtcactcgct catacctgcg cgcttacttg cagcgttatc
     9181 cggagcaaag caacgcctta ttggtgtccc agcgagtcga tcaggtgtat cagcaccagg
     9241 cgttgtttcg cccggagtcg gcgctgcagc cacaagacct tgccgaattg ctcaagcagc
     9301 taaagcgcga taaacagctg gataaattgc tcggacgcta ccatttacgt gatgtgtcgg
     9361 tgctgataaa tgactgaaaa tcctctgttc ggtcagattt gactggggta ggctgcaatt
     9421 attcgaaaaa tagggacgct gacgtcaaat ggggcatttc agacgtaggg taagcaggtt
     9481 tttgggtgtg ggtctggcgt tgttgctgac cgggccactt ttggccgcgc aggatgtgcg
     9541 tgtagccgcc gtgtattttc cgccttacgt attcaagacc gaaaaaagcc aggccaacgg
     9601 tttgcttgat gagctggtag cggcgctgaa ccagacccag acagactacc gcttcgttat
     9661 ggtgccaacc tcggtaaagc gtcgtttccg cgactttgag cagcagcgta tcgacctggc
     9721 gattttcgag aaccccggtt ggggctggca ggagattccg cacgtggcct tggatatggg
     9781 cctggaagac tctgaagtgt ttgtcgcccg tactgagcct ggccgcgagc agggctattt
     9841 tgattcgctc gaaggcaagc gcctggcgct gtaccacggt tatcactatg cctttgccgg
     9901 tttcaattcc ttgccggaat acttgagcaa gaccttcagc gccaccttga cccattccca
     9961 cgaaagtaat ctgctgatgg tgctgcacaa gcgtgcggaa atcgccctgg tgacgcgttc
    10021 ttatattggc gacttccttg agcgcaatcg gcaatacgct gggcaactgc tgatatcaga
    10081 ccgtgtcgat cagcgttatc gccaccatgt gatattaagt ccggaagccc cgatcaagcc
    10141 tgagcagttt atggaaatct atgaagcgtt acgtgataac ggccagttgg agaagatttt
    10201 cagccgctat caggttgccg ttatcccacg cgcagcggat agcttaaaag cagcaggtgc
    10261 agcaaattga cggccccatg cagggcaatg gccaggctga tccgtccgct gaagtgaaag
    10321 atcagcccat agcccagacc cgcgatccct gcgaccacgg caaataacgg actgaaaggc
    10381 aggtgcacgg cagcgaacag cagcgccgtc aggccaatcc ccgcccaggt gccgagccag
    10441 ttgaccaggc ggctttgcag caaaccgcga aatagcagct cctcggccag tacggccaca
    10501 cccaggttga tcgccaacca cagccacaat tgcgctggcc atttcggttg ccagccaacc
    10561 aaacccagcg ccaatgccag cagcggtacg ccgagtagag tcagtgcggc cgttagccag
    10621 acgccggttg aaagcggttt gctcggcgtt ttttgcccca accaccaagc cagtagcgtg
    10681 acgccgacca gcagtttgtc ccaggacaac cgcagggcgt aaggtgcggc atcggcactg
    10741 agctggcgcg gtggccacag cggtgtggcg ctaaaacctg ggacgaggtg cgctgcaaag
    10801 gcgatgcagg ccagcaggct caggacagtc cacagagtat tggacaggta tttgtcggca
    10861 tatagcagca aagcgcaaag agcggccccg gcggccaacc ccggcagctg gatatagccc
    10921 aaggcaaacc cggtggccag gcacagggct ggcagcatca ggcgcagatg caatggcatg
    10981 gtacgtcctt gttgttcgcg gttaaggttc gcgggcgtcc ttggcttcct gttcaagctc
    11041 gcgttggcgg atcttctcca gctgttcctc gcttagcggc agcggtttgg cagtgcgcag
    11101 cagtaccatc aggccaccca cgatggagcc tagcaccagg agtatcagca accagatata
    11161 ccaggtcata aaagccgtcc ttgcagagtt gagcttccac catagcgcgc agcggccagc
    11221 aataaaacgc caataatctt ctgatgggcc taacgcgcgg ctctggctcg gggtaaatga
    11281 gtgtgacgac tgtcacacgg cagcaacttg gcgctggttg aatggcgact ttccaacgca
    11341 cagggagtct gcccatgacc actgccagcg cgctttcgcg cttgctcaag ccggttacgc
    11401 cgttggctgc ggacatgagc atcgccgatg tggcggatcg cctgctgacg ccggagcaca
    11461 aggcctttct ctcgctgccg attgtcgata gcgccggcag gccgctgggc ttggtcagtc
    11521 gcaccacctt gcaggacatc tttatgcagc gttttggccg cgatttgcgc ggccgccatg
    11581 cggtgcagga ggtgatgaac ccggcgccgc tggtggtcag cctggatacc agcttggaag
    11641 atgcggccaa gcagatcacc gggcagctgc agtacccgat caccgaggac tttatcctgg
    11701 tcgacgccca aggcctgtac cgtggcctag gcactgtgct cgatctgctc aaggccatgg
    11761 aggcgcgcat cgcccagcgc aatctggtgc tgcgcaaggc gctggtggaa ctcaaggagt
    11821 cacagaccca gctggtgcag tcagagaaga tggcctcgct cgggcagatg gtcgccggcg
    11881 ttgcccatga gctgaatacg ccgttgggct atgtgaaaaa caacgtgcag ctgctgcgtg
    11941 aactgagtgc gccgctgttc gagctggccg aagcgcaagc cgcactaggc gactgcctga
    12001 acgatccggc ctgtgatgag gcgcgcctgg ccatggccct ggaagccgcc gccgaggccc
    12061 gcgccaaggc tgcaccggaa ctgctggtcg aggactttac ccagttgttc agcgacaccc
    12121 tctatggtct ggagcagatt gccgaactgg tggtcgggct gaaggatttc gcccgcctgg
    12181 atcgcgccat gagtgaggag gtcgacctca atgagtgcat ccgcagcgcc ctggtgattg
    12241 cccgctacgg tatcaaggac aaggccgaaa ccatcttgca gttgggcgaa ctgccacgca
    12301 ttccctgcgc gccttcgcag atcaaccagg tgctactcaa cttgcttaac aacgcggctc
    12361 aggctatcga cggtttcggc cagatcctgg tgaaaacctg ggccgatgac agcgccgtat
    12421 ttgtttctgt gcaggactcc gggcgcggca tgccgcctga ggtgctggcg cgcattttcg
    12481 acccgttttt caccaccaag ccggttggcc agggcaccgg gctggggctg tcgatcagct
    12541 acaagatcat ccaggaccac ggcgggcaga ttcgcgcggc gtccgagcct gggcgcggta
    12601 cgcgctttct gatccgcctg ccgcgcccgc aggcgctgcc actacaacgg agtgcctgat
    12661 atgaacgagc cgattcgtat tctgtttgtc gacgatgaag aacgcattct gcgcagcctg
    12721 gccctgcaat ttcgtcgcga ttaccaggta ctgaccgaga gcgatccgcg tcgcgccctg
    12781 gagcgcctga aaagtgagcc ggtacacata atcgtcagcg accagcgtat gccgcagatg
    12841 agcggcgccg aactactggc cgaggcgcag cagattgctc ccgacacctt gcggattctg
    12901 ttgaccggct acagcgacct ggatgctgcg gtggaggcgc tgaacagcgg ggggatcttc
    12961 cgctatctga ccaagccttg ggaccagcag gaaatggcct ttaccttgcg ccaggcggcg
    13021 cagattgccg tgcggcagtt gcaaacgccg aaagtctcgg cagccatacc gcaggcggca
    13081 ctcaatgtgc tattgctgga tgaggatgcg ccgaccctcg acgtggtggc cgagttctgc
    13141 gtggccggcg gtcatcgctt gttgcgcgcg gcttcgctga acgaggccgt ggcgctgctc
    13201 aacagtcagc cggtcgatct gctggtcagt gatctgcacg tggccgggga agacactgcg
    13261 ccgctgctga aaagcctggc ccaggcccat ccgcgtctac ttagcctggt ggttacgccg
    13321 ttctgcgata cccaggcgct gctcaaactg atcaacgagg cgcagatttt tcgctacctg
    13381 cctaaaccga ttcgacctgg tctgttcgac aagggtttgc aggccgccgc cgaacaggcg
    13441 cagaagtggc gcgcacaacc ggcgcaggtg gtgacccgcc tggcgcaaca gccgcgtgat
    13501 gaccgcgagc gggagaaggt tggcagtttg ctgggcatgc tcgggcgttt gcgtgaacgc
    13561 ctgactgcct gattggacca gagattgatc gcgggcatgg cccgctccta caggtatcag
    13621 cgactgtagg agcgggccat gcccgcgaaa aaaacagtcg ttagcgccgc acctgcttca
    13681 gtgtctcggc aatcaggaac gccagttcca gcgactggtc ggcattcatc cgtgggtcgc
    13741 agtgggtgtg gtagcggtct gacaggctgt cttcggtcac cggccgcgcg ccgccgatgc
    13801 attcggtgac gttctgcccg gtcatctcga tatggatgcc gccggcgtag ctgccctcgg
    13861 cctggtgcac ggcgaagaac tgcttcactt cggcgaggat ctgcgcgaag tcgcgggtct
    13921 tgtagccggt cgaggccttg atggtgttgc cgtgcatcgg gtcggagctc cacagcacct
    13981 gacgaccctc gccctgtacc ttgcgcacca ggcgcggcat atgagcttcg accttgtccg
    14041 cgcccatgcg cacgatcagg ttgaggcggc ctgggtcgtt gtccgggttg aggatatcga
    14101 tcaggcggat cagttcgtcc gggtccatgc tcgggccgac cttgaccccg atggggttgc
    14161 cgacgccgcg caggaactcg acatgcgcac cgtccagctg acgggtgcgg tcgccgatcc
    14221 acagcatatg cgccgagcag gcgtagtcgc cgccggtcag gctgtcgcgg cgcacaaagg
    14281 cctgctcgta gttgagcagc agggcttcat gggcggtgaa gaagctggtt tcgcgcactt
    14341 gcgaggagcc gtccatgcca caggcgcgca taaaggccag ggtttcgtcg atgcgattgg
    14401 ccaggtggct gtacttctcg gccagggccg agttggcgat aaagtccagg ttccactggt
    14461 gcacctgatg caggtcggca aaaccgccct gggcgaaggc gcgcagcagg ttcagcgagg
    14521 cggtggactg gtggtaggcc tgcagcaggc gctccgggtc cggtacgcgg ctggcagagt
    14581 cgaagccgat accgttgacg atatcgccgc ggtaggcggg cagggtcacg ccgtcgatgg
    14641 tttcatcgtt ggccgagcgc ggcttggcga actggccggc catgcgcccg acttttacca
    14701 ccgggcagcc ggcggcaaag gtcatgacga tggccatctg cagcagcacc ttgaaggtgt
    14761 cgcggatttt cgccgcgctg aactcggcga agctctcggc gcaatcgcca ccctgcagga
    14821 gaaacgcgcg accctgggtg acctccgcga actggcggcg cagttcgcgg gcttcaccgg
    14881 caaacaccag cggcggataa ccggccaggg tttgctcgac tttgcccaga tggacggcgt
    14941 ccgggtactg cggttgctgc tggatgggct tggctctcca gctttcgggg ctccagggtt
    15001 gcgacattgc ggctctcggc ttctgacaaa tcgggcggac gggcatggta accgataagc
    15061 ccggcgcttc aagccgatag gcgcgataga gcgactgcat aaggcctagg cagtcactag
    15121 caaagcccca gtacccagta gcaggccata gatggcaatg cggaaatgct gctcgttaaa
    15181 gcgctggtgc agacgttcgc ccagccatac gccgatcaac agcaacggcg cgtagctggc
    15241 gacttgcggc agggcgggca gcaggcggcc gtcgagcaga aaggccagcg tcagcaggct
    15301 gttgagggtg aaccataccg tgaccagggt ggcgcgcagg cgcgccttgt ccagttgcgt
    15361 accggccagg gcgaacacca gcagcgggcc gccggaagca aacagaccgt ggctgacgcc
    15421 agcgcacagg ctaagcaggc ggctgagcca catggggcgt gcgggttgcg cagtaccgtg
    15481 acgcaggcgc catagctcgc gggcggcgaa ccacagcacc agtgcgccaa acaggcgctt
    15541 ggccagcaca gcgtcgagga agggcagcag ggcgtagccg attagggtac cgacgaccat
    15601 acctggcagg atcatgctca gcagtaggcg gcggtcgatc atctgttgat gccgccagac
    15661 caggtaaccg gtcatgcaga tatttagcgg caccagcacc ggcagcagct gatcgatggg
    15721 cagcagcagg gcacccagtg acagggcgat gacgatgctg ccaaaaccgg tgatcgcctc
    15781 cagggtgtac gccagcagga taaaactggc cagcgttaac cagataatgt ccatcagccc
    15841 atctccgcgg cgaagcgtgc gctcatcttg cggtaataaa aaccgggggc gatgcgccag
    15901 agcaggctgg cgagccagct gccgcggtcg ggaaatagcc gctcacgccc ttgctcctgg
    15961 gcttggatga tctgcgcggc catccagtcg gcgccgcgta tttgaccaac ggtcgagcgt
    16021 ccgtgttgcg cctgcttgcc gtcgccgccg agggcgttct gttcgatcgg tgtatcgaga
    16081 aagctgggat acaccagtag catcttgacg ccgtcacggg cggcctcagc gcgcagcact
    16141 tcgaaaaact ggcccagggc gcttttcgca gcgcaatagc cggcacggcc gagcaccggc
    16201 atccagccag ccatggagcc aatggcgatg atctgcccac ggctctggcg cagcagcggc
    16261 agggcgctca gagtcagttc cacgggcgcc tggtagtcga cggccatgac cttgcgaaac
    16321 acggctggat cggtctgcag ggtaggcgag cggtgggtga tcccggcgtt gttgatcagc
    16381 acgtcgaggc ggccgaagcg ttcacaacag gcctgcagta attgctgatg caggtgcggg
    16441 tcggtgatat cgccggccag gccgactacc cgctcggcgc ccagttcatt gacccgcgcg
    16501 ctcaggccgt ctgcgttgat atcggtgagc agcaggttgc agccttggtc gtgataggcg
    16561 cgcgcaagct gccagccgag gccgctggcg gccccggtga tcaggatggt gggcattatt
    16621 tttctccagc gttggccagg gccacaggtg ccagcggcag gcttgcgggt ggccgacggg
    16681 accagataaa ggccagggtc aggccgctga ccaggtgcca gcagccccag aacgcggcga
    16741 tcagcagcat gccgctggcc tgcgggaaga aggtgaagat gatcaccagg cccaaggccg
    16801 agttctggat acccacttcc agggtgatcg cccggcgatc cgctgcgggc aggccggaca
    16861 gtcgtgcgca gccgtaaccc accgccaaag ccagggcgtt gtgcgctacc accagccaga
    16921 agaacagatg gaagtgggcg ataaactggt cgaagttgcg gctaaaggcc agggtgacga
    16981 aggccagcat caccagcagg gcaaagatgc gcagtggctt ttccactttg agcgacaggc
    17041 caggaaaacg cctgccgatc cacatgccgg cgatcaacgg caggccgagc accagcagca
    17101 ccagcagtag caggctgatg gggtccatgg cgatttcagt cagcagcggg cgggtcagtg
    17161 gattaagcca ggcgtagaaa ccgaagttca gtggtgtcag cacactggcc gcgaggctgg
    17221 ataccgcagt catgctcact gatactgcga cgttgccacg tgccatccag gtcatgatgt
    17281 tggagaagct gccgccgggg caggccgcca ccagcatcat gcccagggcc agcatcggct
    17341 cgatgcgtag cgcccagcag gccaggcagg tcagggcggg cagtagaaca aactgcgcga
    17401 acaggccaat cagcggtgcc ttgggttcgc gaacgatgcg tttgaagtca tccaggcgca
    17461 gctccagcga gacgccaaac atcatcaccg cgacgatgac gttgatcagc accaggctgc
    17521 tggggtcgaa ctggatattc accggctcca tcacaggctt ccttcaacag tgcggctggc
    17581 ggtagcgggc agcatagcca ggccggcgcg caggcttttg ctgtgctgcg cgatgctctg
    17641 acgatagctg tccttgtgca cgtagtaggc catacgctcc agttgcaggt attggtagcc
    17701 gccgtcgagg cgcatgctcg cccgttcgcg cttgagtgcc tggaagtcct tggcagcctg
    17761 gctgccagcc tgcaggcctt tgatatacag cgccaccagc tcggcctgct cgtcgcggcc
    17821 ctgccagccg aggcccgagg cttcgaccat gcccagcata aacaggtcgt cgtactcggg
    17881 gtggaacaca ttcaggtaca gctgcggcgc tcctgcgttg ctcggccagt tgaggtgtgc
    17941 gcggtcgatt aaggggtagt cgagcttgta gccggtgcct tgcagaatta ggtcgtactc
    18001 ggcctgctcg ccatcgctga aggtcacttg caggccgtct accttggcta catcgcggcg
    18061 cgcctggata tcgccatgac cgagataatg cagcactagg gagttcatca ccgggtgtga
    18121 ttcgtagagt ttgtagtccg ggtcgggcag gccgtattgc gacggcttgc cgaccaatgc
    18181 gcgcaccagc aggccgtcga ggaactgctt gagcgggcgc ggcagcttga tcgcaccgcc
    18241 gagggtatcg gtcggtcggc cgagggtaaa tttgggtagg aagtaatagc cgcggcgcac
    18301 cgacaggtct accgaggcgg cgcggtgcac ggcgtccacg gcgatatcgc aagccgagtt
    18361 gccgcagccg atcaccagca cgcgcttgcc ttcgaacacc gccgccgagc ggtagtcgct
    18421 ggagtgcatc agctcgcccg taaacgcgcc gggcagcgcc ggctgattgg gtgtgtgcag
    18481 ggtgccgttg gccatcagca cgccgtcgac cagccattca cgctgcacac cgttctgctc
    18541 gctgatcagc ttccagcctt tgtccaggcg ctggattgcc accacacggg tattgaactg
    18601 gtaatgcgca tacaggccga agtgctcggc gtagtcgcgg aaatagcggc gcatttcact
    18661 gtgatgcggg tagggagcca cctcggcgcg catcggaaat tcgctgaact gggtggtgcc
    18721 cttggaggaa atcaggtgcg ccgagtcgta catggtgctg tgcgggttat cgatgtccca
    18781 caggccgccg acatccgcgt gcagttcgaa accgacaaag tcgatgccgt gcttgtgcag
    18841 ttgacgtgct gtacacagcc ccatggggcc ggcaccgata atggcgtaca tggcaaacct
    18901 cgatatttgt ttttattgtt gtagccgcac agcgccgaac tcggcgctcc cgactgcggc
    18961 aaattatcgc gtgaccaggg gagggtgcct caatgacaat tagcgccttt cctctggccg
    19021 agatggtcgg ggatgtcacg gtggagagtg gtggatgcca ggtttacccc cggtcggtca
    19081 gatgccggac ccgctgttga tcgccgaagt gcacgatgcc ttcggggtga tccaggtagt
    19141 cgaaatgggt gcttaccgct ttctcgagtt cggcgaagcg atcgagcaga gctgtgtgta
    19201 tatgccggac cccagctggc tggagtacga ctacacccgc gccatgctgc ttggcgcctt
    19261 gtgccatgag cacccggaaa gcgcgctatt tctcggcctc ggtgccggca acctgaccca
    19321 ggcctgcctg cggtttctgc cgttggaaga tgtcgaagtg atcgagctgc gcgccgatgt
    19381 gccgcgcttg gccatggagc atatgggcct ggtcgatgat ccgcgcctga ccatccgcat
    19441 cggcgatgcg ctggagctgc tggaaagcgc ggaaaaaacc gacctgctgt ttgtcgacct
    19501 ctataccgat cacggcccgg cgaccgggca tctagcctgg cgctttctgg aaaactgcca
    19561 gaagcagctc aaccccggcg gctggctgat tatcaaccag tgggccggca acgacggcaa
    19621 accgcttggc gcaccgctgt tgcgcggcct ctaccatcgg cattactggg agtgtccggt
    19681 gaaggagggc aatgtggtgc tgctggtgcc ggccgatctc gaccagcagc ttgaccagga
    19741 tgagttgctg cgccgcgacg agctgctggc cccgcagctg ggttattcgc tgaggtcgtt
    19801 gatacaggcg ctgcgcccgg cgacctgagc aggtgctggg tcaggtaggg tgcgccgtgc
    19861 gcaccggcgc gggccatggg taattggtgc gcacggcgca ccctacgggt tgggctgctc
    19921 cgcgctcttc agcgcccttt aaagtcgcct gggcggcgct ccaacatggc tttgacacct
    19981 tccttggcat cctcgctggc catcagcctt gtaacagtcg ggtgcaggct ggcggccgca
    20041 gctgtcgggc cttcgactac agcctggcgt gcggaggcca gggtggcctg cacccccagc
    20101 ggcgcctggg cggcaatgcg ttcggcgagc cacagcgctt tgggtagcaa ctcctcactg
    20161 gcgacaactt cctggataaa gcccaggcga taggcttcgt gagcatcgaa ctcatcgcca
    20221 gtcagcagcc agcgcatggc gttgccccag ccagcggtct gatgcatgcg cagggtggcg
    20281 ccgccgaagg ggaagatgcc gcgttgcact tccatctgcg cgaagcgggt attgctggcg
    20341 cacaggttga tgtccgaggc tagcatcagc tcgataccga tggtgtagca gtagccttgt
    20401 gcggcgacga tcaccggttt gctcactcgt ggcccgccga atacgcccca ggggtcgcag
    20461 ccgccgtcag gtatcttcca gccggcggca aactgcgctg ccacattggc caggtccaat
    20521 ccggcggtaa agtgctcgcc atgggcaaat accagcgcca cccgcgcttc gctgtcacgc
    20581 tcgaactcgc cgtaggccag gcacatgtcg tcgagcatgg gcatatcgaa ggcgttgcgt
    20641 ttgctcgctc ggtccaggcc gatcaacatg atctggccgc gtttttcacg gctgacgcga
    20701 ccgctggtgc tttgggtcat ggcggggagt ccttgtgcgc aggttagtaa actggggcgt
    20761 gaagccgtta cagcatggca gctgcaggct atcagaggat gaccatccgg cgtgctatat
    20821 gctcgatttc aggaaaaacc agtacatttg gccaggtttt ccggtatagt gcgcgccggt
    20881 caattcatgg ccacgttcag gtagtacaac tcgcccgaag gctaactttg gcagttagtc
    20941 cgttttgcgg actatccttg acgattcatt tatcacacgt tttcgcaaat ccccgccgac
    21001 atggctgcca gggtgactct cgggtcttac acggcatgcg cagctttgga acatgggtct
    21061 ttgcggatgc actagaggca gacccatgac ccaggaaatc ggcggcttcg ccgctctcga
    21121 tctacatccc aatgttctcg ctgctgtaat tgccactggc tatgaagagc cttcggctat
    21181 tcagattcaa gcgattccag taatcctcgc cggccacgac atgatcggcc aggcgcagac
    21241 cggtaccggt aaaaccgctg ccttcgccct gccaatcctc agccgcatcg acccggccaa
    21301 gcgtcagcca caagcgctga tcctggctcc gactcgtgag ctggcgctgc aggttgccac
    21361 cgcttttgaa acctacgcca agcaaatgcc gggcgtcagt gttatcgccg tttacggcgg
    21421 cgcgccaatg ggcccgcagc tcaaggccat ccgcaatggc gcacagattg ttgtggctac
    21481 tccaggtcgt ctggttgacc acctgcgtcg cgacgagaaa gtgctgtcga ctattcagca
    21541 cctggtcctc gacgaagccg acgagatgct caagcttggc tttatggatg acctcgaagt
    21601 catcttcaag gccatgccgg aaagccgtca gagcgtgctg ttctccgcca ctctgccgca
    21661 ctcgatccgt tccatcgccg aaaaacacct gcgtgaccct aagcacgtca agatcgaaac
    21721 caagacccag accgtaactg ccatcgagca ggtgcatctg atggttcacg ccgaccagaa
    21781 gcacgctgcc gtactgcgtc tgttggaagt cgaagagttc gacgcgctga tcggtttcgt
    21841 ccgcaccaaa caagccaccc tggatctggc cgccgcgctg gaagccaagg gctacaaggc
    21901 cgctgcattg aacggcgaca tcgcacagaa ccagcgtgag cgcgtgatcg actcgctgaa
    21961 agatggccgt ctggacatcg tgatcgccac cgacgttgcc gctcgcggcc tcgacgtgcc
    22021 gcgcatcacc cacgtgatga acgtggacat gccgtacgac ccagaatcct acgtacaccg
    22081 tatcggccgt actggccgtg ccggccgtac cggtcgcgcc ctgctgctgg tgactccgcg
    22141 tgagcgccgc atgctgcaag tgatcgagcg tgtgaccggg cagaaggttg gcgaagtcaa
    22201 gctgccagac gccaagcagg tgctggacgc acgcatcaag aaactcacca acagcctggc
    22261 tccgctggtt gctgatgccg aagccagcca cggtgagcta cttgaccgtc tgaccgccga
    22321 tatcggttgc agcccacgcg ctctggccgc agccctgctg cgcaaggcca ccaatggcca
    22381 agcgctgacc ctggctgaag ttgagcgtga tcagccgctg gtaccgaaca gcagtggccc
    22441 gcgtgagcgt agcgagcgca gtgatcgtcc tgagcgtcct gatcgcgacg gcagccgtga
    22501 gcgccgtgcg ccggtgccgc tggccgaagg ccgcgcccgt tgccgtactg ccctgggcgc
    22561 acgtgacggt atcgccgcga agaacctgct gggtgcgatc ctcaatgaag gcggcctggt
    22621 ccgcgaagcc atcggccgca tccaggtgcg cgagagcttc agcctggtcg agctgccgga
    22681 agaaggcctc gagcgtctgc tgaccaagct gaaagacacc cgcgtcggtg gcaagccact
    22741 gaagctgcgt cgttatcgcg aagattgatt cttcgctgaa taaaaaagcc ccgcctagtg
    22801 tggggctttt ttatgtgttg agttttgcgc cgtagcgctt gcccgccagg tttacctgct
    22861 ggcctgtagg caggccaggt aggagcggac ttgtccgcga taagagttta agggcgactc
    22921 agctgaaact gtagatatcc atccccagtg cgccaagggt gaagccactg tgctcgacct
    22981 tgaactgtcc gcctgcgcct tcggcgaaga acagcggcag caggtgttca tcgctgggat
    23041 ggttgcgcac cgcaaagggt gcttgccggc ggtagtcctg cagggccgcg gtattttggc
    23101 tttccagctg cgcgacgatc cagtcgcgaa acgcctgagc ccagggctcg atgacctcag
    23161 ggcctgcctg ccagtctact tcaccgaggt tgtgggtgat gctgccggag ccgatcagca
    23221 gcacgccctg tgcgcgcagg ctggccaggg cctggccgac ctggtgttgc aaggccgggc
    23281 cgtgctggct gggtagcgag acctgcacca cgggaatatc ggcctgcggg tacatcagcg
    23341 tcagtggcac ccaggcaccg tgatccagcg gtcgttcggc atcgagttgt gctggcagat
    23401 tggctgcggc cagcagttgg attatttccg ccgccagcgt tgggttacca ggcgctgggt
    23461 attgcacttg atagagctcg gcaggaaaac cgccaaagtc atgccaggtt cgcggttgct
    23521 ccgcactacc gacccgcagc tcgcggcttt cccagtgcgc ggaaaccacc actatggcgc
    23581 gtggcctggg cagttggctg gccaggctgg cgagggcggg gccgctggca ccgggttgca
    23641 gggccagcat gggggagccg tgggagataa acaggctggg taacatggcg ttctcctgat
    23701 cgaatgtgtt catcatctat cagtcaatac ggccataaaa gcgcaaatat ccgggttttt
    23761 taatcgggct ggtagattga tccggcctgg ccgatggtcg attggtcttg ttcggcgctt
    23821 tccggcagga ttggccgctg aatatttctc ggagtcgata tgcacgcgga attctggcag
    23881 gcgcgttggg cgcgcagcga aataggtttt catctgtccg aggtcaatcc ctacctgcag
    23941 cgctgctggc ccgacctggc cctggcgcaa ggtacgcggg tgctggtgcc cttgtgcggc
    24001 aagagcctgg atatgctctg gctggccggt cagggttatc gggtgtgcgg ggtcgagttg
    24061 gcgcagaagg cggtggagga ttttttcgcc gaacaccagc tgcagccggt gattagcgag
    24121 gaagggggct tcagggtcta tcgctgtgag gcgctggaga tctactgcgg tgattttttt
    24181 gcgttgacgc cacagcaggt tgccgattgt cagggcctgt atgaccgcgc ggcgctgatt
    24241 gcgctgccgc cggagatgcg cgcacgctat gtcgcgcacc tcgcggccat actgccgccg
    24301 gattgccagg gcctgctggt gaccctggac tacgagcagg cgcagatgga cgggccgccc
    24361 tttgccgttc ccgatagtga ggtgcagcag cgcctggccg gaacctggca ggtggaaacc
    24421 ctggagtgtt gcgatgtgct ggggcagaac tggcgctttc tccagcgcgg tttgcagcgc
    24481 ctggatgagc gggtgtaccg gttgcgcaag cagtaaagcg cggatgaaaa agggcgaccc
    24541 tggggtcgcc cttttatctt gtaccgtgtt tagccgcgct gacgcagggc ttcgatacgg
    24601 tcttccagtg gcgggtgggt catcagcagg ccggccaggc cgtgcttgag accgccgttg
    24661 atgccgaagg cggtcaggct gttgggcatg gtgactggta cttcctgttc ggcgcgcagg
    24721 cgctgcaggg cggcgatcat cgcgccagtg ccagccaggc gagcaccggc ttcgtcggcc
    24781 ttgaattcgc gcttacgcga gaaccacata actatgatgc tggccaggat gcccagtacc
    24841 agctcggcga agatggtcgc gacaaagtag ccaatgcccg ggccgtcttc gttcttgagg
    24901 atgaccttgt cgacgaagtt gccgaagatc cgcgcaaaga acatcacgaa ggtgttcacc
    24961 acgccctgga tcagcgccag ggtgaccatg tcgccattgg ctacatggcc gatttcgtgg
    25021 gccagcacgg cgcgtacttc atcaggcgag aagcgctcca gcaggccttg gctgaccgct
    25081 accagggcat cgttcttgtt ccagccggtg gcgaaggcgt tggactcata ggccgggaag
    25141 atacccactt ccggcatctt gatcccggct tcgcgggaca gttcttccac ggtctgcagc
    25201 agccactgtt cgtgacgggt gcgcggttgg gtgatgattt cggtcttggt gctcatcttc
    25261 gccatccact tggacaggaa cagcgaaatc agcgaaccag cgaaaccgaa tacggcgcag
    25321 aaaaccagca ggctgccata gttttggccg gtaaagcggt cgaccccgag caacttcagg
    25381 gtgatgctgg cgatgatcaa caccgccatg ttggtggcca ggaacagcat aatgcgcatc
    25441 atggtgtatt gagactcctc acgggaaaga tctgtctggt cgctgaaagc cctgtttcgg
    25501 cggcatcgcc tggttttcaa caaccactat tggttatgcc ggctatataa gggcgcaccc
    25561 cccggcactt caacctggcg actatttcaa actatgtcgc ttgcgccatt caggctgctt
    25621 tcgaacagca agcggctgag gcgcgagatc cgttcgccgt gacgctggcc cagcgcctcg
    25681 cgcaactgga aggccagggt ggcatgtacg cggcgcacgc tgggcggcaa ggcctcgcct
    25741 tcgcgcaggg catgggggac ggctcgggtc aggcgcagaa agcccttttc gctaagcacc
    25801 gcctggtcca gggcgtcgta gccgatggtc gaatcgaagc ggatatagcc ttcctcggcc
    25861 agccacagca gggcacccag gcaactttga tggcgtggcg tgggcaggcc gaattcgtcc
    25921 ggctcctcgc ggccgatcag gtcttcgaca tacagtgccg ccttgcgtgg aaatacctgg
    25981 taaaggctca gcaggccggc ggccgcgtcc ttgtagaatt cgtcgatctg caggtccatc
    26041 agaatttgcc aatcagcgaa gtgggcaggt agggcagcag gcggcccagc agggcccagg
    26101 gccagcccgg gacataggcg ctggcggcgc gtttgtcgat atgccgggcg atcagccggg
    26161 ccccgcgctc gacatcaatc agaaacggcc ggttggccat atcgctgttc agcggcgtat
    26221 cgataaagcc cggcaagagc agggtggtgt cgatgttctt gcccagcagt tcggcgcggg
    26281 tggcttccat atagctgatc aggccggcct tgctcgccga gtaggccgcc gtgtgcggca
    26341 ggccgcgttt gccggccact gaagcgacgg cgaccagatg gccgtggccc tgctggcgga
    26401 acagtgcgca ggcggcatcc aggcaggcaa tcgcgccgat caggttggtt tccacggtca
    26461 gtcgggcacg cttgaaatgg ccaccgccgg ctttgccggt cagggcgatg ccggcgttgg
    26521 cgataatccc gtcgagcccg ccgagcaggt tggcggccag gcccacggcg tcctggcagg
    26581 cgtcgtagtc ggtgacatcc agcggcagca ccaccacctt gcgcttaaag cgtgcctgca
    26641 gctcggcggc gagcaggtgc aagctgtctt cgcggcgcgc ggccagggcc aggtcgtagc
    26701 ccttggccgc cagctcgcgg gccagggcgg caccgatgcc agaactggct ccggtcagca
    26761 ggtagcgttt gccctggctc atggcttgct ccgattactg ttggtaagtc ttgaggaagc
    26821 tgccgatacg gccgatggcc atttccagct catccacccg cggcagggtg accacgcgga
    26881 agtgatccgg ccatggccag ttgaacgcgg tgccctggac aatcagcagt ttttccgaaa
    26941 gcagcagatc gaggacgaat ttctcgtcgt tatggatcgg acagaccttc gggtcgatct
    27001 tcgggaaggc atacagcgcg cccatgggct tgacgcaact gaccccgggg atgtcgttga
    27061 gcaactccca ggtgcggttg cgctgctcca gcaggcggcc attgggcaat accaggtcat
    27121 tgatgctctg gtagccgccc agcgcagtct ggatcgcatg ctggctcggc acgttggcgc
    27181 acaggcgcat attggcgagg atatcgatgc cttcgatata gctctgcgca cggtgcttgg
    27241 gtccggaaat cgccacccag ccggagcgga aacccgccac gcggtaggac ttcgacaggc
    27301 cgttgaaagt caggcagagc acgtccggcg ccagcgaggc ggtacagata tgctgggctt
    27361 cgtcgtagag aatcttgtcg tagatttcgt cggagaacag caccaggttg tgctggcggg
    27421 ccagctcgac aatatccagc agcacttctt tggaatacac cgcgccggtg gggttgttcg
    27481 ggttgatcag caccagggcc ttggtgttcg gggtgatctt cgctttcatg tcggcgatgt
    27541 cggggaacca gccggcctgc tcatcgcaca ggtaatgcac cggcttgccg cctgccaaag
    27601 cgactgcggc agtccacagt gggtaatccg gtgccgggat cagcacttca tcgccgttat
    27661 tgagcagcgc ctgcatggcc atcacgatca gctcggacac gccgttgccg aggtagatgt
    27721 cctcgatgcc gacgccttcc acctgcttct gctggtagta ctgcatcacc gctttgcgtg
    27781 cgctgaatag acccttggag tcgctgtagc cctgggcagt cggcaggtta cggatcacat
    27841 cctgcaaaat ttcttcaggc gcttcgaaac cgaacggtgc cgggttgccg atgttcagct
    27901 tgaggatgcg gtgaccttcc tcttctaggc gtttggcgtg cttgagcacc ggcccgcgga
    27961 tgtcatagca gacgttggcg agcttgttcg atttgctgaa ctgcatgtaa gtgatcccga
    28021 atggtggaac gccgctgaat taccctgcaa aaagacgcga tctgacgagc cttggcaggt
    28081 tgtaacgccg gtgacagact ggggtcgaat gatacgtggc agcccgacct tggcaaaggt
    28141 actggttggg cttttttctc tgaaggagac ctatctgtgg acaagctgga aaaacccctg
    28201 gaagcctggc gcgaagagct ttccgaggag cagtttcatg tctgtcgcct gggcggtacc
    28261 gagcgcgcct ttaccggcca gtatcacgac agcaaggtac cgggcattta ccattgcgcc
    28321 tgctgtcagg ccgaactgtt cgactcggat gccaagtacg actccggtag cggctggccg
    28381 agttattttc agccggtcag cagcgaggcc atagtcagcc tggatgactt caatcacggc
    28441 atgcaccgca tcgaagtcaa atgcgccaag tgcgatgctc acttggggca tgtgtttcct
    28501 gatgggccgg cgccgacggg gctgcgttat tgcatcaact cggcatcgct caagctggtg
    28561 ccgaaggctt aaggccatcg cataggcctg cattagcggg ccttatcttg ctttaattaa
    28621 attgcatgca atttaattgc tcgctatctt gcgtccattc ccactcttca ggatgaccag
    28681 catgagcaac gcactcttcg atattccggt taccaccatc aagggcgagc agaagaccct
    28741 ggccgacttc ggtggcaagg ccgtactggt ggtcaacacc gccagcaagt gcggcttcac
    28801 cccgcagtac aagggcctgg agaacgtttg gcagcagtac aaggataagg gcctggtggt
    28861 gctgggcttc ccctgcaacc agttcggcaa gcaggagcct ggtgatgagg gcgcgatcag
    28921 cgagttctgc gagctgaact tcggcgtgag cttcccgctg ttcaagaagg tcgatgtaaa
    28981 tggcagcgat gcccatccgc tgttcgttaa cctgaagaaa agcgcgccgg gcctgctcgg
    29041 cagccagggc atcaagtgga acttcaccaa gtttctggtc agtgccgatg gcttggtgat
    29101 caagcgcttc gccccgacca ccaagcctga agacctgact gccgagattg aagcgctgct
    29161 gaaatgaccc agaccaccct gcacgcggat gacgggctgc agctggacaa ccagctgtgc
    29221 ttcaagctct atgccgcttc acgggcggtg atccgcgcct acaagccgat gctcgatcag
    29281 ctcggcctga cctacccgca gtacctggcc atgctggtgc tgtgggagtg gcagcagcaa
    29341 ccgccagcgt tgccgacggt caaggccttg ggtgagcgcc tattgctcga ctccggcacg
    29401 ttgacccctc tgctcaaacg cctggagctg caaggcttgt tgcagcgcaa gcggtcggcc
    29461 agtgatgagc gtgaagtgca ccttgcgctg accccggccg gatgcgcctt gcgtgagcag
    29521 gtgttgccgc tgaaaagcca gctgctgtgc gaacgcggga ttgatctcga tcaggtaacc
    29581 gagttgcgta gccaggtcgg tgtgctgctt gatcagttta tggtgcttaa ccactgatcg
    29641 agcagggctg ccaactcatc cttgcgaaag ggcttggcca ggtagtcgtc cattcccgca
    29701 gcgcggcagc gttcgcgttc atcggacagt gcgttggccg tcagggcgat gatcggcagc
    29761 tcggcgaatt tggttttttg gcgaatccgt tgggtggttt catagccatc catgaccggc
    29821 atattgcagt ccatcagaat cagatcgacc acctgggtct ccagcaggct cagggcttcg
    29881 ccgccgtgat tggccagcag cacctcgcag ccgagcttgc ccagcatgcc cttggctacc
    29941 agttggttga ccgggttgtc ttccaccagt agtacacggg cctgacgatt gcgttgcggc
    30001 tcgctgtgct gctcggcggc atgcgcttcg ccctgaccca aggtgcggcg caaggcttgc
    30061 agcagcgcct ggcgtgacag tggtcgggcc agttgttcga taggcgagag tttctccacc
    30121 tgctcgctgc tgagaaagtt gccgtaggcg ctgacgagca gaatcggcag cttgcagagt
    30181 gtgcgcagtt cactcagacg ctgtgggcaa tcgctgatca gcagatcggc ttcaagctgg
    30241 cctagcgcgg catcggcgtc caggtgctga tactgcaggc cccagctcgg cagcaggctt
    30301 gccagttgtt cgctgagccc ggagctggct ggactaagcc cgagaatgcg tcctttcagt
    30361 acggggcgtt gttgggcgtt ggcctggccg agtagtggca ggtcgatgca gaaggtgctg
    30421 ccgaagcctt cccgtgtctc caggctcagt tggccatgca tggcttcgca gaggcggcgg
    30481 gtcagggcca gacccaggcc ggtgccgcca aattgtcgag tgatccccac cccagcctgg
    30541 gcaaacgggt ggaagatccg cgtctgggcg tcttcggcaa tgccgatgcc ggtgtcgcgg
    30601 acaataatcc tgacgccacc ggtctgggcg ctcacgctga tatcgacacg accaaagcgg
    30661 gtgaatttca acgcgttgga cagcaggttg ctaacaatct gcctgacccg ggtcgggtcg
    30721 ccgagcagtt ggttgggcag ttgtgggtcg atcaggcaag tcagttcaac accgggggcg
    30781 gcgttctgcg acagcaggtt ggcggtgtct tcgaccaagc tcgccagatc gaaaggaatc
    30841 tgctccagtt ccagctgccc ggcctcgaat tttgacaggt cgagaatgtc gttgagcagc
    30901 tcgaccagca ctttgccgga gtcgtgggca attgacagct gctggcgctg ttcgttattc
    30961 agcggtccat ccagtgacag cgcgagcata cctagcaggc cattgagcgg ggtgcgaatc
    31021 tcgtggctca tattggccag gaaggccgag cgcgcctggg ccatgtccag tgccgtgcgt
    31081 ttggcttgct ccagctcgcg gttggagtcg agcaggcggc tgttggcggc ttccagctgc
    31141 tcggtgcgcg ccgagaccat gtcttccagc tcggccaggt agcgggtcag gcgggcctcg
    31201 gcttgctggc gctgctcgat ctcgctggcg atgctgctca gttggcggtt ggtcacatcg
    31261 accagtacgc cgatctcgtc gcgttcatgg ttgggcgggt agggcagctt ggcgttattg
    31321 gggctgcgca ggtcacgttc gctgagggcg cggataaaac cggtgagcgg cttggtcagc
    31381 atgaaataga acaacaccag caggatcagt gacagcagca ggctgcgggc gaagccggtg
    31441 agcagggtca gtagcgagcg gttgaggaag tggctgccaa aggcgtaggt gtcgacttcc
    31501 aggctcagct tgccgagggc ttcgctggga gaatggctga cgaacagggc ggtctcgaaa
    31561 tgtcggcgct tgccaaacat aaagtcgctg agtacccggt aacggctttc catggcgggt
    31621 cggctgaccc tggccaacgt cagttggttg ttgtcgatga tttgcgcgct gatcacagca
    31681 ggtgagcgca gcaggcccag caccagttcc tgtgccagtt cggcgtcgat gttgtaggcg
    31741 atacgtgccg ccgggttgtg gctgatttcc atcagcgcgt taatctcgcg gttgatggag
    31801 gcatcttcgc tggcataatc gatgcccact tgaatcaagc tgagcgccgt accaaggata
    31861 aaggctacca atacggtcaa gccggcctgt ttgaatgaca ggcgttgggt tagcgctata
    31921 tccatatggg ccggttgttc tcgtgttgag tcgcttgcca agcatagccc atctgttttg
    31981 ccggacggca gtggcagagt tgcttgaaaa ccctggagtg tgttgtggat tcgcgtttaa
    32041 gtgatttttt gttgcgcgct gaggctgtaa tggcccgctt agagccctta ttgccgatac
    32101 agcgtccggt tatcgactgg cagcagagcc tggcggcacg ttggcagcgc gatggtcgca
    32161 gtggttttct cgcgccgctg caggtcagtc tggatgtgaa tctagatgat ctgatcgggg
    32221 tcgatcgtca gcgggcccag ttggcgcgca atacccagca gtttatccag ggtctgccgg
    32281 ccaaccatgc gttgctctgg ggcgcgcgtg gtacgggtaa gtcatcgctg gtgcgggcgc
    32341 tgctggccga gcatgcggca gcggggctgc gtctgatcga gattgaacgt gatcatttgg
    32401 ccgatctgcc gcgtatcgtc gagcagctga gcgagctacc gcagcgtttt gtgttgttct
    32461 gcgatgacct gtcgttcgag gccggtgagg gtgattaccg ca
//
LOCUS       DASRUM010000130        19457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_578194, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000130 DASRUM010000000
VERSION     DASRUM010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 19457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19457
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578194"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFF22_08850"
     CDS             <1..316
                     /locus_tag="VFF22_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16953.1"
                     /translation="SRTPGAFVYTEVQVSVPFDQAPWRTMNPILRAQPGLLHKTWLSG
                     VDNQSVGGFYAFDTLENARRFVTDYFPTETAGLNAAYTTRLFDASATEVASREMGSQF
                     YR"
     gene            complement(329..697)
                     /locus_tag="VFF22_08855"
     CDS             complement(329..697)
                     /locus_tag="VFF22_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011105017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HZX16954.1"
                     /translation="MRLLLIAITSLLLSACASQSQVDPQGYRAEGQASYYGARHHGNK
                     TASGERFDQNQLTAAHRTLPFGSRVRVTNLNNDKSVVVRINDRGPYTRGRIIDLSQKA
                     AEQLNMLRAGVVPVRVERVD"
     gene            complement(769..2217)
                     /gene="gatB"
                     /locus_tag="VFF22_08860"
     CDS             complement(769..2217)
                     /gene="gatB"
                     /locus_tag="VFF22_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HZX16955.1"
                     /translation="MQWETVIGLEIHAQLSTQSKIFSASAIAFGAEPNTQASLVDLGM
                     PGTLPVLNAEAVRMACKFGLAIDAEIAQSNVFARKNYFYPDLPKGYQTSQMDHPIVGK
                     GFLDITLEDGTQKRIGITRAHLEEDAGKSLHEDFHGMSGIDLNRAGTPLLEIVSEPDI
                     RSAKEAVAYVKAIHALVRYLGICDGNMAEGSLRCDCNVSVRPKGQAEFGTRAEIKNVN
                     SFRFIEKAINHEVQRQIELIEDGGKVVQETRLYDPNKDQTRSMRSKEEANDYRYFPCP
                     DLLPVVIEQSFLDEIRASLPELPVEKRARFEREFGLSAYDADVLAASREMANYFEAVN
                     TVCGDAKLAANWVMGELSSLLNKDNLEIDQSPVSAEQLGGMILRIKDNTISGKIAKMV
                     FEALAAGEGASADEVIEKKGLKQVTDSGAIESMLDEVLAANAEQVEQYRAADEAKRGK
                     MFGFFVGQAMKASKGKANPGQVNELLKKKLED"
     gene            complement(2229..3680)
                     /gene="gatA"
                     /locus_tag="VFF22_08865"
     CDS             complement(2229..3680)
                     /gene="gatA"
                     /locus_tag="VFF22_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HZX16956.1"
                     /translation="MHQFTLAEIARGLADKQFSAEELTRNLLARIAQLDPQLNSFITV
                     TEELALSQAKAADARRAAGESGALLGAPIGHKDLFCTQDVLTSCGSKILTGFKAPYNA
                     TVVDKLAAAGAVTLGKLNMDEFAMGSANESSHYGAVKNPWDLTRVPGGSSGGSAAAVA
                     ARLLPAATGTDTGGSIRQPAALTNLTGIKPTYGRVSRWGMIAYASSLDQGGPLARTAE
                     DCALMLQAMAGFDTKDSTSVDQPVDDYLASLSQPLNGLRIGLPKEYFGAGLDARIGEK
                     VMAVVEELKKLGATVKEIALPNMQHAIPAYYVIAPAEASSNLSRFDGVRFGYRCENPV
                     NLEDLYKRSRGEGFGAEVKRRIMVGTYALSAGYYDAYYLKAQKIRRLIKNDFVAAFND
                     VDVILGPTTPNLAWKLGEKNNDPVAAYLEDIYTITANLAGIPGLSMPAGFVDGLPVGV
                     QLLAPYFQEGRLLNVAHQYQQVTDWHTQAPAGF"
     gene            complement(3697..3984)
                     /gene="gatC"
                     /locus_tag="VFF22_08870"
     CDS             complement(3697..3984)
                     /gene="gatC"
                     /locus_tag="VFF22_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZX16957.1"
                     /translation="MALERSDVEKIAHLARLGLNEADIPGTTATLNNILGLIDQMQAV
                     NTDGIEPLAHPLEATQRLRADQVTERNQREAYQAIAPAVENGLYLVPKVIE"
     gene            4251..5291
                     /gene="mreB"
                     /locus_tag="VFF22_08875"
     CDS             4251..5291
                     /gene="mreB"
                     /locus_tag="VFF22_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HZX16958.1"
                     /translation="MFKKLRGMFSSDLSIDLGTANTLIYVRERGIVLNEPSVVAIRTS
                     GNNQKSVVAVGTEAKRMLGRTPGNIQAIRPMKDGVIADFSVCEKMLQYFINKVHENSF
                     LQPSPRVLICVPCKSTQVERRAIRESALGAGAREVFLIEEPMAAAIGAGLPVEEARGS
                     MVVDIGGGTTEIALISLNGVVYAESVRVGGDRFDEAIITYVRRNYGSLIGESTAERIK
                     QEIGTAYAGGELREVDVRGRNLAEGVPRSFTLNSNEVLEALQESLATIVQAVKSALEQ
                     SPPELASDIAERGLVLTGGGALLRDLDKLLSQETGLPVIVAEDPLTCVARGGGKALEM
                     MDRHTMDLLSSE"
     gene            5380..6339
                     /gene="mreC"
                     /locus_tag="VFF22_08880"
     CDS             5380..6339
                     /gene="mreC"
                     /locus_tag="VFF22_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZX16959.1"
                     /translation="MKPLFAKGPSLGVRLLVFTVLSAALMVVDARFTLLQPLRAQLGL
                     IVEPVYWVGRLPVRVWESATQELSTRNELAAENEKLKAEQLMMQRRLQKLAALTEQNV
                     RLRELLNSAALVDDEVLATELIGIDPNPFTHRILIDKGEKDGVVLGQPVLDARGLMGQ
                     VVEVMPYTSRVLLLTDTTHSIPVQVNRNGLRAIASGTGNPERLELRHVADTADIKEGD
                     LLVSSGLGQRFPAGYPVAIVSEVIHDSGQPFAIVRAVPTATLNRSRYMLLVFTDPRTP
                     EERATESAQAQEALDREILQQAQPAQPEADAPVAPANPPEAAQ"
     gene            6336..6824
                     /gene="mreD"
                     /locus_tag="VFF22_08885"
     CDS             6336..6824
                     /gene="mreD"
                     /locus_tag="VFF22_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HZX16960.1"
                     /translation="MMAARSSNLWVVWLTFALALLLSVSSMPKFMELGRPLWLALFLT
                     YWVLALPHRVGMTTAWVIGLLADVLNGTLLGQNALILTLITFLVLTLHQRLRMFPMWQ
                     QSTVLLVVFGLAQLVQLWLNALTGSRPPTLAFVLPALVSALLWPWVCTLLRGLHQRLG
                     VN"
     gene            6870..7490
                     /locus_tag="VFF22_08890"
     CDS             6870..7490
                     /locus_tag="VFF22_08890"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HZX16961.1"
                     /translation="MATLYLASGSPRRRELLTQIAVPFLTQIAPIDENALPGESPIAY
                     VERLARAKAQAGLTALSDTADAVVLGADTAVVLDGRILGKPADRDDALATLSALSGRS
                     HQVLTAVALISRERLESRVVTSQVTFRALSQAEIEAYWASGEPQDKAGCYGIQGLAAV
                     FVSQLQGSYSAVVGLPLCETAALLAEFAIPCWQSLPVHSQEVAGRG"
     gene            7552..9009
                     /gene="rng"
                     /locus_tag="VFF22_08895"
     CDS             7552..9009
                     /gene="rng"
                     /locus_tag="VFF22_08895"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HZX16962.1"
                     /translation="MSEEILINITPMESRVAVVENGVLQEVHVERTQKRGIVGNIYKG
                     KVVRVLPGMQAAFIDIGLERAAFIHAAEISSREGPAVESISALVHEGQSLVVQVTKDP
                     IGSKGARLTTQLSIPSRYLVYMPRTAHVGISLKIEDEAERERLKQVVADCVAAEGIEE
                     AGGFILRTAADGAGADEILADIRYLRRLWEQIATQMKVGPTPLVIYEDLSLALRTLRD
                     LVNPKIEKIRVDSRETFQKITQFVDELMPEIADRLEHYPGERPIFDLYGVEDEIQKAL
                     ERKVPLKSGGYLIIDPAEAMSTIDVNTGAFVGHRTLEETIFKTNLEAATAIARQLRLR
                     NIGGIIIIDFIDMEDEEHQRQVLRTLEKQLERDHAKTNIIGITELGLVQMTRKRTRES
                     LEQVLCEPCSCCQGRGKLKTAETTCYEIFREILREARAYQPEGYRVLANQKVVDRLLD
                     EESGNVADLEAFIGRTIKFQVETMYSQEQYDVVLL"
     gene            9041..12874
                     /locus_tag="VFF22_08900"
     CDS             9041..12874
                     /locus_tag="VFF22_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HZX16963.1"
                     /translation="MTVLARWVALLLRTALGLCALALVLAALYVSLGRELVPLVAEYR
                     LEAENKARAALAMPVQIGALEGLWQGFSPVLTAHDVAIGEGAGALQLDQVRVVPDVLA
                     SLLARQLRVARLELDGVQLVVQQAEDGHWALKGLPQRNEAAPLDLAQVLTQSQMVKRL
                     SLFNSQVTVQAFEQPARTLTYVNLTLRNGSARQRLDGRLLLPDGQPLALQLRTRMSAK
                     RWKEVEAELYLSLPQSDWAAWVPASLLAQWRLEQLQLGGEVWLSWAKGGVQRAVSRLH
                     VPQLKAGYAKRESVALNNLALNAYFTRTELGFKLLLDDLAFSRDDKRWGEVRVALTQQ
                     AESAEVEEQWLLSADRLDLAPLQPLVAGLAPLPDKGLALLEQLKPHGALRNIQLDYRP
                     QLSDSKRLQFSANLERVGFAAYLGSPAAENVSGSISGDLGQGELRVDSENFSLHLDTL
                     FPKPWHYQKANALLTWQIDEQAFTLRSPYLQVLGEEGPAAGDFLIRLRFDPTQEDYMD
                     LRVGLRNGDARFTEKYLPSRAPGMSADLDAWLKSAIRGGAVDEGYFQYQGSLNKGAEN
                     TSRSISLFFAVHDAELAFQPGWPALREGRGRVLIEDTGVRVELAQGRILDSQVSNAQA
                     QIATALPGQPPRLLLTSELSSNIDDALHILQQTPIGTAETFAGWTGAGALAGKLNLDL
                     PLRKGMAPAVVVDFATEGAQLSLPNPPLQFSQLQGAFRYNTASGLSAPDIRAQVLGHA
                     VRGKALAEGARGKARSRIEASGQIAVSELSTWLGVTQPLPISGNLPYRLNLTLDGADS
                     QLRVSSSLKGATVSLPAPFGKTAAEERATQWRMTLGGREQRYWLDYADLLSLTFAASP
                     GQFNQGRGEIRLTDGPASLPTSQGLRLRGRVAELDWSAWQQVLQPYASVPVDDAQRLF
                     KDAQLRIGRFTGFGISLDALEVSVKRDAATWAVSLDSPQLKGQVGLPDARELPIALTL
                     DYLRFPAAEKKTAEEAAVDAPDPLASVDPSSFPALDVRIAEVYQGIEPLGSWSLKARP
                     VSAGVQFSELDLNLKGLKVAGRATWLKAANGTRTSYDGRMGGADLADVLIAWGFAPTA
                     SSRDFHMNVDGHWPGSPAWFSMKRFSGSLDASLRKGQFSEVEGPASALRVFGLLNFNS
                     IGRRLRLDFSDLLGKGLSYDRVKGLLVADNGVFVTREPITLTGPSSNLELNGTLDMAN
                     DRIDAKLLVTLPVTNNLPLAALIVGAPAIGGALFIADKLLGDRVARFASVQYDVKGAW
                     QNPDISFDKPFEKPQ"
     gene            12960..13808
                     /locus_tag="VFF22_08905"
     CDS             12960..13808
                     /locus_tag="VFF22_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZX16964.1"
                     /translation="MTFAVIQMVSQADVLANLAQARQLLEQAAAQGARLAVLPENFAA
                     MGRRDQAALGRAEALGEGPILPWLQQAARDLGLWIVAGTLPLPPDGQPDAKAHACSLL
                     IDNAGQRVARYDKLHLFDVDVSDSYGRYRESDDYAFGGQVVVVDTPVGRLGMTVCYDL
                     RFPELYSALRAAGAELISVPAAFTAVTGAAHWQILTRARAIETQCYVLAAGQGGTHPG
                     GRETFGHSALVDPWGRVLAELPQGPGVLLAERDAAEQAAIRQRMPVARHKRFFAAAEL
                     RLPGLE"
     gene            13811..15268
                     /gene="tldD"
                     /locus_tag="VFF22_08910"
     CDS             13811..15268
                     /gene="tldD"
                     /locus_tag="VFF22_08910"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016781785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HZX16965.1"
                     /translation="MNNMLVSVSEHLLGPGGLTIEHLPAVLADLAGPGIDAADLYFQS
                     QVSETWALEDGIVKEGSFNLDQGVGVRAQSGEKTGFAYSNAITADALSQAARAARSIA
                     RAGQNGRVQAFSSPLVKPLYAQDNPLDVLGRAEKVELLKRIDVATRALDSRIKQVSVS
                     LAGVWDRILVAANDGSLGADIRPLVRFNVSVIVEQNGRRERGSHGGGGRTDYRYFLEQ
                     TSNNEERAMGYAREALRQALVNLEAIPAPAGTMPVVMGAGWSGVLLHEAVGHGLEGDF
                     NRKGSSAYSGRMGEQVASSLCTIVDDGTLAERRGSLSMDDEGTQTQCTTLIENGVLKG
                     YMQDKLNARLMGVACTGNGRRESYAHLPMPRMTNTYMLAGESDPEEIIRSVKKGIYCA
                     NLGGGQVDITSGKFVFSTSEAYLIEDGKITAPVKGATLIGNGPEAMSKVSMVGNDLAL
                     DSGVGTCGKDGQSVPVGVGQPTLKIDAITVGGTGG"
     gene            complement(15319..15837)
                     /gene="yjgA"
                     /locus_tag="VFF22_08915"
     CDS             complement(15319..15837)
                     /gene="yjgA"
                     /locus_tag="VFF22_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HZX16966.1"
                     /translation="MSEYLDDFSGEKSKTQVKREFHALQDLGQRLTTLKLDLLNKLPL
                     TDELRRALAEAPKHTANAAKKRHVQFIGRLMRDQDIPAILTLLDQLDASTRQYNERFH
                     NLERWRDRLLNGDDDTLEKFVGEYPDADRQHLRQLIRQGQHELAQNKAPAASRKIFKY
                     IRELDETQRGLR"
     gene            15941..17287
                     /gene="pmbA"
                     /locus_tag="VFF22_08920"
     CDS             15941..17287
                     /gene="pmbA"
                     /locus_tag="VFF22_08920"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003171795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HZX16967.1"
                     /translation="MSAVDIVGPQAVPQLQAQVEQIIAEAKKQGASACEVAVSVEQGL
                     STTVRQGEVETVEFNRDQGFGITLYIGQRKGSASTSASGEAAIRETVAAALAIAKHAS
                     EDECAGLADAALMARELPQLDLYHAWSITPEQAIEQALSCEAAAFAADQRIKNADGTT
                     LNTHQGCRVYGNSHGFVSGYASTRHSLSCVMIAEDGGQMQRDYWYDVNRQGELLADAG
                     SIGRRAAERAVSRLGARPVPTCEVPVLFAAELATGLFGSFLAAISGGNLYRKSSFLEG
                     SLGQQLFPEWLKIDERPHIPRALGSAAFDGDGLATYAKSFVERGELLSYVLGTYSGRK
                     LGMPSTANSGGVHNLFVSHGDEDQKALLKRMGRGLLVTELMGQGLNMVTGDYSRGAGG
                     YWVENGEIQFPVQEVTIAGNMRDMFKQIVAVGNDLELRSNIRTGSVLIERMTVAGS"
     gene            complement(17354..17626)
                     /locus_tag="VFF22_08925"
     CDS             complement(17354..17626)
                     /locus_tag="VFF22_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HZX16968.1"
                     /translation="MPACEITIINKLGLHARAAAKFVGVAGRFPCQVRVGRTPESLVD
                     GKSIMAVMMLAAGKGTPVHLHTEGEQDAEALQALSELINNYFDEGE"
     gene            complement(17639..18496)
                     /gene="rapZ"
                     /locus_tag="VFF22_08930"
     CDS             complement(17639..18496)
                     /gene="rapZ"
                     /locus_tag="VFF22_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HZX16969.1"
                     /translation="MRLIIVSGRSGSGKSTALDVLEDNGFYCIDNLPAGLLPELAERA
                     LLHTELLHPQVAVSIDARNLPSHLQRFPELLDEVRARHIKCDVIYLDADEETLLKRFS
                     ETRRRHPLTNESRSLAEAIADEALLLGPIIDLADLKIDTTHLNLYQLRDSLKLRLLNQ
                     PEPGTAFLVESFGFKRGMPVDADLVFDVRCLPNPYWKPDLRDFSGLDQPVVDYLSAQA
                     DVEDMYQDILAYLTKWLPRFAASNRAYVTVAIGCTGGHHRSVYLANRLGETLKPILKN
                     VQTRHRDLS"
     gene            complement(18499..18972)
                     /gene="ptsN"
                     /locus_tag="VFF22_08935"
     CDS             complement(18499..18972)
                     /gene="ptsN"
                     /locus_tag="VFF22_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HZX16970.1"
                     /translation="MIRLETILTPGRSLVNVPGGSKKRVLEQIANLVARELPDLDGQD
                     IFESLIAREKLGSTGFGNGIAIPHCRLPGCTAPISALLRLDAAVDFDAIDGAPVDLLF
                     VLLVPEAATDAHLELLRQIASMLDRSDVRASLRQAESSEALYQVVVDVQSQLNRA"
     gene            complement(18988..19296)
                     /gene="raiA"
                     /locus_tag="VFF22_08940"
     CDS             complement(18988..19296)
                     /gene="raiA"
                     /locus_tag="VFF22_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HZX16971.1"
                     /translation="MQVNISGHQLDVTDALRDYIAEKLGRLERHFDKITNVQVTMEVE
                     KLKQKIEATLHIAGGEVVANAEHEDMYAAIDLLTDKLDRQLIKHKEKQLERQQGAMAR
                     "
BASE COUNT         3444 a         5591 c         6424 g         3998 t
ORIGIN      
        1 cagtcgcacg ccgggtgcat tcgtctacac cgaagtgcag gtttcggtcc cgtttgatca
       61 ggcgccttgg cgcaccatga acccaattct tcgggcgcag cctgggttgc tgcacaaaac
      121 ctggttgagt ggtgtggata atcaatccgt cggtggcttt tatgccttcg atacgttgga
      181 aaacgcccgc cgtttcgtca ccgattactt cccaaccgaa actgcggggc tgaatgcggc
      241 ctacaccacg cgcttgttcg atgcgtcggc gaccgaggtg gcaagtcgcg aaatgggttc
      301 gcagttttat cgttaacctc gttcgtcctt agtcgacacg ttctacccgg accggaacga
      361 cgcctgcgcg cagcatgttg agttgctcgg cggctttttg ggagaggtcg ataattcgcc
      421 ccctggtata ggggccgcgg tcgttgatgc gcaccaccac gcttttgtcg ttgttgaggt
      481 tggtcacgcg gactcgtgaa ccgaagggta gggtgcggtg ggctgcggtc agttggttct
      541 ggtcgaaacg ctcgccgctg gcggtcttgt tgccgtggtg gcgcgcgccg taataggacg
      601 cctggccctc ggcacggtaa ccctgcgggt ctacctggct ctggctcgcg caggcgctga
      661 gcaacaggct ggtgatggcg attaacagaa ggcgcatggc ggctccatga tgagggctgc
      721 gaacaaaaca acgccgggct gacccggcgt tgtgtattac agcaaggctc aatcttcgag
      781 ctttttcttc agcagttcgt tcacctggcc ggggttggcc ttgcctttcg aggccttcat
      841 ggcctgaccg acgaagaagc cgaacatctt gccgcgcttc gcttcatcag cggcgcggta
      901 ttgttcgacc tgctcggcgt tggctgccag cacttcgtcc agcatgcttt cgatggcgcc
      961 ggagtcggtg acctgcttaa ggcctttctt ctcgatcact tcatcggcgc ttgcgccttc
     1021 accagcggcc agggcctcga agaccatctt ggcgatcttg ccgctgatgg tgttgtcctt
     1081 gatgcgcagg atcatcccgc ccagctgctc ggcggaaacc ggcgactggt cgatctccag
     1141 gttgtccttg ttgagcaggc tggacaactc gcccatcacc cagttggcgg ccagcttggc
     1201 gtcgccacac acggtgttga ccgcctcgaa gtaattggcc atctcgcggc tggcagctag
     1261 cacgtcggcg tcataggcgg acagaccgaa ctcacgctcg aagcgcgcgc gcttctcgac
     1321 tggcagctct ggcaggctgg cgcggatttc atcgaggaag ctctgctcga ttaccaccgg
     1381 cagcagatcc gggcagggga agtaacggta gtcgttggct tcttccttgc tgcgcatgga
     1441 gcgcgtctgg tccttgttcg ggtcgtacag gcgagtttcc tgcaccacct tgccgccgtc
     1501 ttcgatcagc tcgatctggc gctgcacttc gtggttgatg gctttttcga taaagcggaa
     1561 cgagttgacg tttttgatct cggcgcgagt gccgaactcg gcctggccct tgggccgcac
     1621 cgagacgttg cagtcgcagc gcagcgagcc ttcggccata ttgccgtcgc agatgcccag
     1681 ataacgcacc aacgcgtgga tggccttaac gtaggccacg gcttccttgg ccgaacggat
     1741 atccggctcg gaaacgattt ccagcagcgg cgtgccggcg cggttgaggt cgatgccgct
     1801 catgccgtgg aagtcttcgt gcaggctttt gccggcgtct tcttccagat gcgcgcgggt
     1861 gatgccgatg cgcttctgcg taccgtcttc cagagtgatg tcgaggaagc ccttgccaac
     1921 gatggggtga tccatctggc tggtctggta gcccttaggt aggtctgggt agaagtagtt
     1981 tttgcgcgca aagacgttgc tctgcgcgat ctcggcatca atcgccaggc cgaatttgca
     2041 ggccatgcgc acggcttcgg cgttgagcac cggcagggtg ccgggcatgc cgaggtcaac
     2101 caggctggcc tgggtgttgg gctcggcgcc gaaggctata gcgctggcgg agaaaatctt
     2161 cgattgggtg ctgagttggg cgtgaatctc cagcccgatt acggtttccc attgcatatc
     2221 agtcgtcctc agaatccagc cggagcttgt gtgtgccagt cagtaacctg ttggtactga
     2281 tgggcgacgt tgagcaggcg gccttcctgg aagtaagggg cgagcagttg cacaccgacc
     2341 ggcaaaccat cgacgaagcc agcaggcatc gacaggccgg ggatgccggc gaggttagcg
     2401 gtgatggtgt agatgtcttc caggtaggcg gcgaccggat cgttgttctt ctcgccgagt
     2461 ttccaggcca ggttcggcgt ggtagggccg aggatcacgt cgacgtcatt gaacgcggcg
     2521 acgaagtcgt tcttgatcag ccggcggatt ttctgcgcct tgaggtagta ggcgtcgtag
     2581 taaccggcgg acagcgcgta ggtgccgacc ataatgcggc gtttaacctc ggcaccaaag
     2641 ccttcgccgc gcgagcgctt gtacaggtct tccagattca ccgggttctc gcagcggtag
     2701 ccgaagcgca cgccgtcgaa acgcgacagg ttggagctgg cttccgccgg ggcgattacg
     2761 tagtaggcgg gaatcgcgtg ctgcatattt ggcagggcga tttccttgac ggtcgcgccg
     2821 agctttttca gctcttcgac aacagccatc accttctcgc cgatacgtgc gtccaggccg
     2881 gcgccgaagt attccttcgg caggccgata cgcaggccat tcagtggctg gctaagggag
     2941 gcgaggtaat cgtcgaccgg ctgatcgacg ctggtcgagt ccttggtatc aaaaccggcc
     3001 atggcttgca gcatcagcgc gcagtcttcc gcggtgcggg ccagtgggcc gccctggtcg
     3061 aggctggagg cgtaggcgat catgccccag cgggagacgc ggccgtaggt tggcttgatc
     3121 ccggtgaggt tggtcagcgc tgccggttgg cggatcgagc cgccagtgtc ggtgccggtg
     3181 gcggctggca gcaggcgtgc agccactgca gcagcggagc cgccagaaga gccgccgggt
     3241 acgcgggtca gatcccaggg gtttttcact gcgccgtagt ggctggattc gttggccgag
     3301 cccatggcga attcgtccat gttcagctta cccagggtca cggcaccggc ggcggcgagc
     3361 ttgtcgacca cggtggcgtt atacggggcc ttgaaaccgg taaggatctt cgagccgcag
     3421 ctggtcagta cgtcctgggt gcagaacagg tccttgtggc cgatcggtgc gccgagcagg
     3481 gcgccgctct cgccagccgc gcggcgcgcg tcggccgctt tggcctggct cagggccagc
     3541 tcctcagtga cggtgataaa gctgttgagc tgcgggtcaa gttgggcgat gcgcgccaac
     3601 aggttgcggg tcagttcttc agcggaaaac tgcttgtcgg cgagtccgcg ggcgatctcg
     3661 gccagggtaa attgatgcat ggctaggctt tttcccttac tcgatgactt tcggaaccag
     3721 atacaggccg ttttccacgg ccggggcgat ggcctgatag gcctcgcgct ggttgcgttc
     3781 ggtgacctgg tcggcgcgca ggcgctgggt ggcttccagc gggtgagcca gcggctcgat
     3841 gccgtcggta ttgactgcct gcatctggtc gatcaggccg agaatgttat taagggtcgc
     3901 agtggtaccc gggatatcgg cttcattcag gcccagtcga gccagatgag cgattttctc
     3961 cacgtcggag cgttcaagcg ccatcaggtt tctccctata taaagcagcg caatcgctgg
     4021 ccgcctttgg aaatgtgaac gggtcgccat gcacgggtgc gcagggaaca gatcgtgaca
     4081 ggtggcggtc aaaggccgcg atgatgggcc ttaaagcccg gaaaaaccgg caatctaaca
     4141 tattggtgcc ttgctcaaaa tccctgccgt tgttagagtt tgccgcactt tttagcagcg
     4201 cctttatcgc gctttgcctt tacccacgcg ttgcctaggg tccctatccc atgttcaaaa
     4261 aactgcgtgg catgttttcc agcgatctgt cgattgacct gggcactgcc aataccctga
     4321 tttatgttcg cgagcgcggc attgtgctga acgagccgtc cgtggtcgcc atccgtacca
     4381 gcggtaacaa ccagaagagt gtggtcgccg tcggtaccga ggccaagcgc atgctcggtc
     4441 gtaccccggg caacatccag gccatccgcc cgatgaaaga cggcgtgatc gccgacttca
     4501 gcgtctgcga gaagatgctg cagtacttca tcaacaaagt tcacgaaaac agcttcctgc
     4561 agccatcgcc gcgcgtgctg atctgcgtgc cgtgcaaatc cacccaggtg gagcgccgcg
     4621 ccatccgtga gtcggccctc ggtgccggtg ctcgtgaagt gttcctgatc gaagagccaa
     4681 tggctgctgc cattggtgcc ggtttgccgg ttgaagaagc ccgcggttcg atggtcgtcg
     4741 atatcggtgg tggtaccact gaaatcgcgc tgatctcgct gaacggtgtg gtgtacgccg
     4801 aatccgtccg cgttggcggc gaccgtttcg acgaagcgat catcacctac gtgcgccgca
     4861 actacggctc gctgatcggt gagtccaccg ctgagcgcat caagcaggaa atcggcaccg
     4921 catacgccgg tggcgaactg cgtgaagtcg acgtacgtgg ccgcaacttg gccgaaggcg
     4981 taccgcgcag cttcaccctg aactccaacg aagtgctcga agcgctgcag gaatccctgg
     5041 cgaccatcgt ccaggcggtg aaaagcgcgc tggagcaatc gccgccagaa ctggcctccg
     5101 acattgccga gcgtggcctg gtgctcaccg gtggtggcgc actgctgcgt gatctggaca
     5161 aactgctgtc gcaggaaacc ggtctgccgg tgatcgtcgc cgaagacccg ctgacctgcg
     5221 ttgcacgcgg cggcggcaag gctctggaaa tgatggatcg ccacaccatg gacctgctgt
     5281 cctcggagta aggcgccact gttccaagtc ctaagctgca accgggctct acccgcttgc
     5341 agctttttgt ttgctgcctg acgccgtgag gagctgccga tcaaaccgct atttgccaaa
     5401 ggtccttcgc tgggtgtgcg cctgctggtg ttcaccgtgc tgtcggctgc gctgatggtg
     5461 gtcgatgcgc gcttcaccct gctgcagccg ctgcgtgcgc agttgggcct gattgtcgag
     5521 ccggtctact gggtcggtcg cctgccggtg cgggtgtggg agagcgcgac tcaggaactc
     5581 agcacgcgca atgagctggc cgccgagaat gaaaagctca aggccgagca gctgatgatg
     5641 cagcggcgcc tgcagaagct tgccgccctc accgagcaga acgtgcgctt gcgtgagctg
     5701 cttaactcgg ctgcgctggt cgacgacgag gtgctggcca ccgagctgat cggtatcgat
     5761 cccaacccct ttacccaccg catcctgatc gacaagggtg aaaaagacgg cgtggtccta
     5821 ggccagccgg tactggatgc gcgtggcctg atgggccagg tggtcgaggt catgccgtac
     5881 acctcgcgtg tgttgttgct gacggacacc acccacagca ttccggtgca ggtcaatcgc
     5941 aatggcctgc gcgccatcgc cagcggcacc ggtaacccgg agcggctgga gctgcgtcat
     6001 gtggcggaca cggcggatat caaagagggc gatctgctgg tcagctccgg ccttggccag
     6061 cgttttccgg ccggttatcc ggtggccata gtttccgaag tgatccacga ctccggccag
     6121 ccctttgcga ttgtccgtgc agtgcccacg gctacgttga atcgcagccg ctacatgttg
     6181 ctggtgttta ccgacccgcg tacaccggag gagcgcgcca ctgaatcggc gcaggcgcag
     6241 gaagccctgg atcgtgaaat tctgcagcag gcgcaacccg cgcaacccga ggccgatgcg
     6301 ccagtggcgc ccgccaatcc gccggaggca gcgcaatgat ggccgcacgt tcaagcaatc
     6361 tctgggtggt ctggctaacg ttcgccttag ccttgttgct cagtgtgtcg agcatgccga
     6421 aatttatgga gttgggtcgg ccgctgtggc tggcgctgtt tctcacctat tgggtgctgg
     6481 ccttgccgca tcgtgtgggt atgaccacgg cctgggttat cggcttatta gccgatgtgc
     6541 tgaatggcac attgctcggc cagaatgcgc tgatcctgac cctgattacc ttcctggtgc
     6601 tgaccctgca tcagcgcttg cgtatgttcc ccatgtggca gcaaagcact gtactgctgg
     6661 tggtgtttgg cctcgcgcag ttggtgcagc tgtggctgaa tgcgctgacc ggcagtcgcc
     6721 cgccaaccct ggcgtttgtc ttgccggcct tggtcagtgc cttgctctgg ccctgggttt
     6781 gcaccttgtt acgcgggctg catcagcgtc tgggcgtcaa ttaagtaagg ccgctgggcc
     6841 ttgcgtttat ccgacaggga gaagtcagca tggccaccct ttatctcgcc tccggatcgc
     6901 cgcgtcggcg tgaactgctg acgcaaattg ctgtgccctt cctcacgcaa atcgccccca
     6961 tcgacgaaaa tgcattgcca ggtgagtcgc caatcgccta tgtagagcgt ctggctcgcg
     7021 ccaaggcgca ggctggactg actgcattga gcgatactgc ggatgccgtg gtgctcggcg
     7081 ccgatacggc ggtggtgctc gatgggcgga ttctcggcaa gcctgccgat cgcgacgatg
     7141 cgttggcgac attgagtgct ctgtccggac gcagccatca ggtgctgacg gccgtggcgc
     7201 tgattagtcg cgaacggctg gagtcgcggg tggtcacaag ccaggtaacc ttccgcgcgt
     7261 tgagtcaggc cgaaatcgag gcctactggg ccagcggcga gccgcaggac aaggccgggt
     7321 gttacggtat tcagggcctg gcggcggtgt ttgtcagcca gttgcagggc agttattcgg
     7381 cagtggtggg tttgccgctg tgtgagactg ctgcgttgct cgcagaattt gctattccgt
     7441 gctggcagtc gctgccagtt cacagtcaag aggttgcggg tcgcgggtga atgacagcct
     7501 ttaccggcgc agtccctggg ctttcagctt tatctcagaa gagatgagcg catgagtgaa
     7561 gagatcctga tcaacatcac gccgatggaa tcgcgcgtgg cggtggtgga aaacggcgta
     7621 ctgcaggaag tgcacgtcga gcgcacgcag aagcgtggca tcgtcggcaa tatctacaag
     7681 ggcaaggtgg tgcgcgtgct gccgggcatg caggcggcct ttatcgacat cggcctggag
     7741 cgtgcggcgt ttattcatgc cgcggaaatc tccagccgcg aaggccccgc cgtggaaagc
     7801 atcagcgccc tggtgcatga aggccagagc ctggtggtgc aggtcaccaa ggacccgatt
     7861 ggcagcaagg gcgcgcgcct gaccacccag ctgtcgatcc cttcgcgtta tctggtgtat
     7921 atgccgcgca ccgctcacgt cggcatctcg ctgaagattg aagatgaagc tgaacgcgag
     7981 cgcctgaagc aagtggtggc cgattgcgtg gccgccgaag gcatcgaaga agccggcggc
     8041 tttattctgc gtaccgccgc tgacggtgcc ggggccgatg agatcctcgc cgacatccgt
     8101 tacctgcgcc gcctgtggga acagatcgcc acgcagatga aagtcggccc gacgccgctg
     8161 gtgatctatg aggatctgtc cctggcgctg cgtactctgc gtgacttggt caacccgaag
     8221 atcgagaaga tccgcgtcga ctcgcgggaa accttccaga agatcactca gttcgtcgat
     8281 gagctgatgc cggaaatcgc cgaccgcctg gagcactacc caggtgagcg gccgatcttc
     8341 gatctgtatg gcgtcgagga tgaaatccag aaggcgctgg agcgcaaggt gccgctcaag
     8401 tctggcggtt acctgattat cgatccggcc gaggcgatga gcaccatcga tgtgaatacc
     8461 ggcgcgttcg tcggccaccg cacgctggaa gagaccatct tcaagaccaa cctggaagcc
     8521 gccacggcga ttgctcgcca gctgcgcctg cgcaatatcg gcgggatcat catcatcgac
     8581 tttatcgaca tggaagatga ggagcaccag cgccaggtgc tgcgtaccct ggagaagcag
     8641 ctggagcgcg accacgccaa gaccaatatc atcggcatca ccgagttggg cctggtgcag
     8701 atgacccgca agcgcacccg cgaaagcctc gagcaggtgc tgtgcgagcc gtgcagctgc
     8761 tgtcaggggc gcggcaagct gaaaactgcg gaaaccacct gctacgaaat cttccgggaa
     8821 attctgcgtg aggcccgcgc ctaccagccg gaaggttatc gcgtgctggc caatcagaaa
     8881 gtggtcgacc ggctgctcga tgaagagtcg ggcaatgtcg ccgatctcga agcctttatc
     8941 ggtcgcacca tcaagttcca ggtcgaaacc atgtattccc aggagcaata cgatgtggtg
     9001 ctgctctgat atgcgcgctt ctcgcctggg cgtgaactga atgacggtat tggcgcgctg
     9061 ggttgccctg ctgctgcgta cggcactggg gttgtgcgcc ctggcgctgg tgttggcggc
     9121 gctgtatgtc agcctcggcc gtgagctggt gccgctggtt gccgaatacc gcctggaagc
     9181 cgaaaacaaa gcccgcgcag cgctggccat gcccgtgcag atcggtgcgc tggaagggct
     9241 ctggcaaggt ttttcaccgg tactgaccgc tcatgacgtg gcgatcggtg agggcgctgg
     9301 ggccttgcag ctggatcagg tgcgcgtggt ccctgatgtg ctggctagct tgctcgcgcg
     9361 gcagttgcgt gttgctcggc ttgagttgga tggcgtgcag ctggttgtgc agcaggcaga
     9421 agacggtcac tgggcgctca agggcttgcc gcaacgcaat gaagccgccc cgctcgacct
     9481 ggctcaggtg ctgacgcagt cgcagatggt caagcgcttg tcgctgttca atagccaggt
     9541 caccgtgcag gccttcgagc agcccgccag aactctgacc tatgtaaacc tcactctacg
     9601 taacggcagt gcgcgccagc gtctggacgg ccgcctgctg ttgcccgatg gccagccgct
     9661 ggctctgcag ttgcgtacgc ggatgagcgc caagcgctgg aaagaggttg aagccgagct
     9721 gtacctgagc ttgccgcaga gcgattgggc ggcctgggtg cctgcgagcc tgttggcgca
     9781 atggcggctt gagcagttgc agctgggcgg cgaggtctgg ctgagctggg ccaagggcgg
     9841 tgtgcagcgc gccgtcagtc gcctgcatgt gccgcagctc aaggccggct atgccaagcg
     9901 cgagtcggtg gcgctgaata atctggcgct gaatgcctat ttcacccgca ccgagctggg
     9961 tttcaagttg ctgctggatg acctggcctt tagccgtgat gacaagcgct ggggcgaagt
    10021 gcgggtggcg ctgacccagc aggcggagag cgcagaggtc gaggagcagt ggctgctgag
    10081 tgccgatcgc ctcgacctgg caccgctgca accgctggtg gcggggcttg cgccgttgcc
    10141 ggataaaggc ctggcgttgc ttgagcaact caagccgcat ggcgcgctgc gcaatattca
    10201 gctcgattac cggccgcagc tcagcgacag caagcgcttg cagttctcgg ccaatctgga
    10261 gcgcgtcggc tttgccgcat acctcggctc gccggctgcg gagaacgtca gcggcagcat
    10321 cagcggtgat ctgggccagg gtgaactgcg cgtcgatagc gaaaactttt ccctgcacct
    10381 cgacactctg ttccccaagc cctggcacta ccagaaagcc aatgccctgc tgacctggca
    10441 gattgatgag caggcgttca ccctgcgcag cccgtatctg caggtactgg gtgaggaagg
    10501 ccctgccgcc ggtgatttcc tgattcgtct gcgcttcgat ccgacgcaag aagactatat
    10561 ggacctgcgc gtcggcctgc gtaacggcga tgcgcgcttt actgagaaat acctgccaag
    10621 ccgggcgccg ggtatgagcg ccgatctgga tgcctggttg aaaagtgcca ttcgtggtgg
    10681 cgcggtcgat gaaggctatt tccagtacca gggctcgctg aataagggcg cggaaaatac
    10741 ctcgcgcagt atcagcctat tcttcgccgt acatgatgcc gagctggcct tccagcccgg
    10801 ctggccggcg ttgcgcgagg gccgtgggcg ggtcttgatc gaagacacgg gtgtacgggt
    10861 cgagctggcg caagggcgca tcctcgatag ccaagtgagc aacgcccagg cgcagatcgc
    10921 aaccgcatta cccggtcagc caccacgcct gttgttgacc agcgagctga gcagcaatat
    10981 cgatgacgct ctacatatcc tgcagcagac gccgataggc accgcagaga cctttgccgg
    11041 ctggactggt gcgggggcgc tggccggcaa gctcaacctt gatctgccgc tgcgcaaggg
    11101 catggcccca gcagtggtgg tggattttgc cacggaaggc gcgcagctca gcctgcccaa
    11161 tccgccgttg cagttcagtc agttgcaggg cgcatttcgc tacaacactg ccagtggcct
    11221 tagtgccccg gacattcgcg cccaggtgct cggccatgcg gtgcgtggca aagccctggc
    11281 cgagggggcg cgcggcaaag cgcgtagccg cattgaggcc agcgggcaga tcgccgtcag
    11341 tgagctgagc acctggctgg gcgtgaccca gccgctgccc atcagcggca atctgccgta
    11401 tcgcctcaat ctgaccctgg acggtgccga cagtcagctg cgtgtgagtt cttccctcaa
    11461 aggcgcgacg gtgtcactgc ctgcaccgtt cggcaagacc gctgctgagg agcgcgcaac
    11521 ccagtggcgc atgaccctgg gtgggcgtga gcagcgctac tggctggatt acgctgattt
    11581 gctgagcctg acctttgccg cgagccctgg tcagttcaat cagggccgtg gcgaaattcg
    11641 cctgaccgat ggcccggcca gcttgcccac cagccagggc ttacgacttc gcgggcgggt
    11701 ggcggagctg gattggtcgg cctggcagca ggtgttgcag ccctatgcca gtgtgccagt
    11761 tgatgatgcg cagcggctgt tcaaggatgc gcagctgcgt attggccgct tcaccggctt
    11821 tggcatcagc ctggatgcac tggaagtgtc ggttaaacgc gatgccgcaa cctgggcggt
    11881 cagcctcgac agcccacagc tgaaaggcca ggtcggtttg ccggacgccc gtgagctgcc
    11941 gattgccctg acgctcgact acctgcgctt cccggcggca gagaagaaga ctgcagaaga
    12001 agctgcggtg gatgcgccag acccactggc aagcgttgac ccgagcagct tcccggcctt
    12061 ggatgtgcgt attgccgagg tttatcaggg catcgaaccg ctgggcagct ggtcgctcaa
    12121 ggcgcgcccg gttagcgctg gtgtgcagtt cagcgagctt gatctcaacc tcaaaggctt
    12181 gaaggttgcg ggccgggcca cctggctaaa ggccgcgaac ggcactcgaa cgagttatga
    12241 cgggcgcatg ggcggcgctg atctggccga tgtgctgatc gcctggggct ttgcacccac
    12301 cgccagcagc cgtgactttc atatgaatgt ggacggtcac tggcccggct cgccggcatg
    12361 gttcagcatg aaacgcttta gcggctcgct ggatgcttcc ctacgcaaag ggcagttttc
    12421 cgaggttgaa ggtccggcgt cggcgctgcg ggtgttcggg ctgctgaatt tcaactcgat
    12481 tggccgacgc ctacgcctgg acttctccga tctgctcggc aaaggcctga gctatgaccg
    12541 ggtcaagggt ctgttggtgg ccgataacgg tgtgtttgtt acccgtgagc cgatcaccct
    12601 tactgggcca tcgagcaatc tggagctcaa tggcacgctg gatatggcca atgaccgcat
    12661 cgatgccaag ttgttggtga ccttgccggt gaccaacaac ctgcctttgg ccgcgttgat
    12721 agtcggtgcg ccggccattg gtggggcgtt gtttatcgcc gataagctgc tgggtgaccg
    12781 cgtggcgcgc tttgccagtg tgcagtacga cgtcaaaggc gcctggcaga acccggatat
    12841 cagtttcgac aagcccttcg aaaaacctca gtaatccccg cacggttgat atgcagccgt
    12901 gcagtctgcg ggtatggctt gtaagctgaa gccataccgg cattgagata aaggcagtca
    12961 tgaccttcgc agtcattcaa atggtcagcc aggccgatgt cctggccaat ctggcgcagg
    13021 ctcggcagtt gctcgaacag gccgctgcgc aaggtgcacg gctggcggtg ctgccggaaa
    13081 atttcgctgc catgggccgg cgtgaccagg ccgcgctggg ccgtgccgaa gcgctgggtg
    13141 aggggccgat tctgccctgg ttgcaacagg ccgcccgcga tcttggcctg tggattgttg
    13201 ccggcacctt gcccttacca ccggatggcc agcccgacgc caaggctcac gcctgctcgc
    13261 tgctgatcga taatgccggc cagcgcgtgg cccgttatga caagctgcac ctgtttgatg
    13321 tggatgtcag cgacagctat ggccgctatc gcgagtctga cgattacgcc ttcggcggcc
    13381 aggtggtggt ggtcgatacc ccggttgggc ggttgggcat gacggtgtgt tatgacctgc
    13441 gcttccccga gctgtacagc gccttgcgcg ccgcgggagc ggagttgatc agcgtgccgg
    13501 cggcctttac tgcggttacc ggcgcggcgc attggcagat actcacccgc gcgcgggcga
    13561 tcgaaaccca gtgctatgtg ctggcggccg ggcagggcgg tactcatcca ggcgggcgcg
    13621 agacctttgg ccattcggcg ctggtcgacc cctggggccg cgtgctcgcc gaactgccgc
    13681 aggggccggg cgtgctgctg gccgagcgtg atgcagctga gcaggcggcg atccgtcagc
    13741 gcatgccggt ggcgcggcat aaacgatttt ttgcagcggc cgagttgcgg ctgccaggtt
    13801 tggagtagtt atgaacaata tgttggtgtc cgtcagtgaa catctgctgg ggccgggcgg
    13861 tttgaccatt gagcatttgc cggcggtcct ggctgacttg gccgggccag gaatcgatgc
    13921 ggccgatctg tatttccaga gccaggtttc ggaaacctgg gcgctggagg atggcatcgt
    13981 caaggaaggc agtttcaacc tcgatcaggg tgttggtgtg cgcgctcagt cgggtgagaa
    14041 aaccggtttt gcctacagta atgcgatcac tgccgatgct ttgagccagg ccgcacgtgc
    14101 ggcccgttcg attgcccgtg ctgggcagaa tggccgcgtg caagcgttca gcagcccgct
    14161 ggtcaagccg ctgtatgccc aggacaaccc gctggatgta ctggggaggg cggaaaaggt
    14221 cgagctgctc aagcgcatcg acgtcgccac tcgcgccctg gattcacgga tcaagcaggt
    14281 ttcggtcagc ctggctgggg tctgggatcg cattttggtg gccgccaacg acggcagcct
    14341 gggcgcggat atccgcccgc tggtgcgctt caacgtcagc gtgatcgtcg agcagaacgg
    14401 tcgccgcgag cgcggcagcc atggcggcgg cggacgtacc gattaccgct acttcctcga
    14461 gcagaccagc aataacgaag aacgcgccat gggctatgcc cgtgaggccc tgcgccaggc
    14521 gctggtcaac ctcgaagcga ttccggcacc ggcaggcact atgccggtgg tgatgggcgc
    14581 aggctggtcc ggtgtgctgc tgcacgaagc ggtcggccat ggcctggaag gcgacttcaa
    14641 tcgcaagggc agctctgcct acagcggccg gatgggtgag caggtggcct ccagcctgtg
    14701 caccatcgtc gatgacggca ccctggccga gcgtcgcggc tccctgagca tggacgacga
    14761 aggcacacag acccagtgca ccacgctgat cgagaacggc gtgctcaagg gttatatgca
    14821 ggacaagctg aacgcccgct tgatgggcgt ggcatgcacc ggtaatggtc gccgtgagtc
    14881 ctacgcgcac ctgccgatgc cgcgcatgac caatacctac atgctggccg gcgagagcga
    14941 cccggaagaa attatccgtt cggtgaagaa aggcatctac tgcgccaacc ttggcggcgg
    15001 tcaggtggat atcaccagcg gcaagttcgt gttttctacc agcgaggcct acctgatcga
    15061 ggacggcaag atcaccgccc cggtcaaagg cgccaccctg attggcaatg gcccggaagc
    15121 catgagcaag gtgtcgatgg tcggtaacga cctggcgctg gacagcggcg tcggtacctg
    15181 cggtaaagat gggcaatcgg tgccggttgg cgtgggccag ccgacgctga agatcgatgc
    15241 gattaccgta ggcggaacgg gcgggtgaga ttggtaggat gggttagccg aaggcgtaac
    15301 ccatcgtgac cgagaggcct agcgcaagcc gcgctgggtt tcatcaagtt cgcggatgta
    15361 tttgaagatt ttgcgtgacg cggcaggcgc tttgttctgc gccagttcgt gctggccctg
    15421 acggatcagc tgacgcaggt gctggcggtc agcgtcgggg tattcgccga caaatttttc
    15481 cagggtgtcg tcgtcgccgt tgagcaaacg gtcacgccag cgctccaggt tatgaaagcg
    15541 ttcgttgtac tggcgggtgg aggcatccag ctggtcgagc agagtcagga tggcggggat
    15601 atcctgatcg cgcatcaagc ggccgataaa ctgcacatgg cgcttcttcg cggcattggc
    15661 agtgtgcttg ggcgcttcgg ccagcgcgcg gcgcagctcg tcggtcagcg gcagcttgtt
    15721 cagcaggtcg agtttcaggg tagtcaggcg ttgccccaga tcttgtagcg catgaaactc
    15781 gcgcttgacc tgggttttgc tcttctcgcc ggagaagtcg tcaaggtatt cagacatggt
    15841 ggcggtccag tggaaaacgc cgacatgata gcagcaagtt tccagctgta agcgtctggc
    15901 ctatttggcc gcgtttgccg cttatcgttc aggagttctt atgagtgcag tagacatcgt
    15961 cggcccccag gctgtgccgc aattgcaggc gcaggtggag cagattattg ccgaggctaa
    16021 aaaacagggc gcgagcgcct gcgaggtggc cgtatcggtg gagcagggct tgtccaccac
    16081 ggtgcgtcag ggtgaggtgg aaacggtcga attcaatcgc gaccagggct ttggcatcac
    16141 tttgtatatc ggtcagcgca agggctcggc gagcacctcg gccagcggtg aagcggcaat
    16201 ccgcgaaacc gtcgcggcgg ccctggcgat tgccaagcat gcctccgaag atgaatgcgc
    16261 tggtttggcc gatgccgcgc tgatggcccg tgaactgccg cagttggatc tgtaccacgc
    16321 ctggtcgatc acccccgagc aggccatcga acaggccttg agctgcgagg cggcggcctt
    16381 tgccgcagac cagcgaatca agaatgccga cggtacgacg ctgaataccc atcagggctg
    16441 ccgggtctat ggcaacagcc atggctttgt cagtggctat gccagcacgc ggcacagctt
    16501 gagctgcgtg atgatcgccg aagatggtgg gcagatgcag cgcgactact ggtatgacgt
    16561 taatcgccag ggcgagttgc tggccgatgc cggcagcatc ggccggcgtg ccgccgagcg
    16621 tgcagtaagc cgtttgggtg cgcgcccggt gccgacctgc gaagtgccgg tgctgttcgc
    16681 cgccgagttg gccaccggtt tgtttggcag ctttctggcg gccatttccg gcggcaatct
    16741 gtaccgcaag tcatcgtttc ttgaaggctc cctagggcag cagctgttcc ccgagtggct
    16801 gaaaatcgat gagcgcccgc atattcctcg tgcgctgggc agtgccgctt tcgatggtga
    16861 cggtctggcg acttacgcca agtcctttgt cgagcgtggc gagctgctgt cctatgtgct
    16921 cggtacctac tctgggcgca agctgggtat gcccagcacc gccaactccg gtggggtaca
    16981 taatctgttt gtcagccatg gcgatgaaga ccaaaaggcg ctgctcaagc gcatggggcg
    17041 cgggctgcta gtgaccgagc tgatggggca gggcctgaat atggtcaccg gcgactattc
    17101 gcgcggtgcc ggtggctact gggtggagaa cggcgagatc cagttcccgg tgcaggaagt
    17161 caccatcgcc ggcaatatgc gtgacatgtt taagcagatt gtcgcggtgg ggaatgacct
    17221 ggagctgcgc agcaatatcc gcaccggctc ggtgctgatc gagcgcatga cggtggcggg
    17281 tagctgattc cgcttgtatg aaaaaggggc tgtcgcttaa gcgttcagcc cctttttctt
    17341 gtctctcgat cagctattcg ccttcgtcga agtagttgtt gatcagctcg ctcagggcct
    17401 gcagcgcttc ggcgtcctgc tcgccttcgg tgtgcaggtg caccggcgtg cctttgccgg
    17461 cagcgagcat catcactgcc atgatgctct tgccgtcgac cagactctcc ggcgtgcggc
    17521 cgactctgac ctggcagggg aaacgcccgg cgacgccgac aaacttggcc gccgcgcggg
    17581 cgtgcaggcc aagcttgttg ataatggtga tttcgcaggc gggcatcgcg gcaattcctt
    17641 aactgaggtc gcggtggcga gtctgaacgt ttttcaggat gggcttgagg gtttctccca
    17701 ggcggttggc caggtacacc gagcggtgat ggccgccggt gcagccaatc gccacggtga
    17761 cataagcacg gttgctggcg gcaaagcgcg gcagccattt ggtcaggtag gcgaggatgt
    17821 cctggtacat gtcctcgaca tcggcctggg ccgagaggta gtcgactacc ggttggtcga
    17881 ggccagagaa atcgcgtaga tccggcttcc agtacgggtt gggcaggcag cgcacatcga
    17941 ataccaggtc agcatccacc ggcatgccgc gcttgaaacc aaaggactcg accagaaacg
    18001 cggtacccgg ctcgggctgg ttaagcaggc gcagtttgag gctgtcgcgc agctgataca
    18061 ggttgaggtg agtggtgtcg atcttcaggt cggccaggtc aatgatcggc ccgaggagca
    18121 gcgcttcatc ggcgatggct tcagccagtg agcgactctc gttggtcagc gggtggcgcc
    18181 gacgggtttc cgagaagcgt ttgagcaggg tttcttcatc ggcatccagg tagatcacat
    18241 cgcacttgat gtgccgcgcg cggacctcat caagcagttc ggggaagcgc tggaggtggc
    18301 tcggcaggtt gcgcgcatca atcgacaccg cgacctgcgg atgcagcagt tcggtgtgca
    18361 gcagagcacg ctcggccagt tcaggcagca gcccggcggg cagattgtcg atgcagtaga
    18421 aaccgttgtc ctcaagcaca tcgagggcgg tgctcttccc ggagccggag cgaccgctaa
    18481 cgatgatcag gcgcatgctt aggctcggtt gagttggctt tgtacgtcca cgaccacctg
    18541 atacagggct tcgctgcttt ctgcctgacg caggctggcg cgcacatcgc tgcggtcaag
    18601 catgctggca atctgccgga gtagttccag gtgtgcgtcg gtggccgctt cgggtaccag
    18661 cagaacaaac agcaggtcca ctggtgcgcc gtcgatggcg tcgaagtcca cggcggcgtc
    18721 caggcgcagc aacgcgctga tcggcgcggt acaaccgggc agacgacagt gcggaatggc
    18781 aatgccatta ccgaaaccgg tcgagccgag tttttcgcgg gcaatcaggc tttcgaagat
    18841 gtcttggccg tccaggtcag gcagctcgcg cgccaccagg ttggcaatct gttcgaggac
    18901 acgttttttg ctgccgcccg gcacgttcac caaggaacgg ccgggggtca ggatagtttc
    18961 aagtcggatc ataggatata gacgatgtca gcgggccata gcgccctgtt gacgttcgag
    19021 ctgtttttcc ttgtgtttga tgagttgtcg gtcgagttta tcagtcagta gatcgatggc
    19081 tgcgtacatg tcttcatgct cagcattggc gacgacttcg ccgccagcaa tgtgcagggt
    19141 cgcttcgatt ttctgtttga gcttctcgac ctccatggtg acttgtacgt tggtgatctt
    19201 gtcgaagtgg cgctccaatc ggccgagttt ttcggcgatg tagtcgcgta gggcgtcggt
    19261 cacatccagc tggtgtccac tgatgttgac ttgcataccg ctttctcctt gttgccgtgt
    19321 gtaagaggca ggcatgaggc ctgccgccgg aacactgtgg cgtggaagac cctggcagaa
    19381 cgagtctggc actggcgcaa atcccttgtg ggcagtatgg ctgctttcga gaagatgcgt
    19441 ggccgggtgt tgtgtgc
//
LOCUS       DASRUM010000131         6867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1246332, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000131 DASRUM010000000
VERSION     DASRUM010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246332"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..1140
                     /locus_tag="VFF22_08945"
     CDS             280..1140
                     /locus_tag="VFF22_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZX16972.1"
                     /translation="MRNLKNLCLQSLGVAALALGMSSAMAADKPTLNIGYVNGWDDSV
                     AATFVAGEILKSNLGYSVELKPVEPAIMWQGVARGDLDATLSAWLPATHGEYFEKLKD
                     KVEVLGTNYAGAKIGLIVPDYVQAKTIADLQTYAKDFDGKITGIDAGAGVMRRAEEAI
                     KEYALTDIKLMPSSGPAMATALTRAEKNKKAIVVTGWIPHWMFAKWNLRFLEDPKKVF
                     GDDEHVDTVANPSLNSKAPEAVAFLKKFSWSGEEVGAVMLAIREGAKPEDAAKAWISK
                     NPERVAEWLK"
     gene            1317..2090
                     /locus_tag="VFF22_08950"
     CDS             1317..2090
                     /locus_tag="VFF22_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16973.1"
                     /translation="MDSKKKCGMCSSSEPLQNSHIIPRSYFRSLKNGCGQLYEVSSSE
                     KVKSKLTNSDPKEELLCLNCEQFLSLTYEKYGTRLLKDEKIKYGKAFVIFKSFRYKEF
                     YLYLISILWRASISKIARYDHIDLGNSINSLLCHCVKNNTIKIQTSLRLDHFFRISLI
                     RIKDDTKKLDDNILRKAMFDINSEPSKNREDGVIYYFMVDGFLIIYHISCEDDTHKLR
                     TKKNYAQISNTTNMKVPVSDISNFKQIADGFNSIRSKSN"
     gene            2258..2419
                     /locus_tag="VFF22_08955"
     CDS             2258..2419
                     /locus_tag="VFF22_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007942592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2986 domain-containing protein"
                     /protein_id="HZX16974.1"
                     /translation="MNRRKKIKQLLEAHAKKANAKLAPKKKSTYISKADRLKLADEPN
                     QDLAITPEN"
     gene            2452..2808
                     /locus_tag="VFF22_08960"
     CDS             2452..2808
                     /locus_tag="VFF22_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16975.1"
                     /translation="MFSRITIIILCLLLVASNIFWLYSAIDSGVTRAYMGQQIYEANN
                     SSDQAVGMLKKLLTGKTKSEVQALAQEFSDIEAFEKEGCLWVGWYGFKFSDADTLIDI
                     EPKPGYSEKSACNSEL"
     gene            2872..3477
                     /locus_tag="VFF22_08965"
     CDS             2872..3477
                     /locus_tag="VFF22_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16976.1"
                     /translation="MIIVVEGVSAAGKTTWASRHSPAVVSELTGHPPDTDNVTALGQY
                     WSDRHSERWQHGLALEHVHKVVCFDTDPLKIHYAWCLWQIGKDSRAAWMADVEASRER
                     VAQKRLGFADQIVFLEPPEEVVRQQKANDKVRRRSNFETHVRLYEPLRRWYMLLETLS
                     PGRVVFNAHQAPDSTSMTLQADRYSLQLFDALIDAADQPPK"
     gene            3597..4124
                     /locus_tag="VFF22_08970"
     CDS             3597..4124
                     /locus_tag="VFF22_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZX16977.1"
                     /translation="MNVLILPGIGNSGPEHWQSHWEQANSRYTRVHQADWDNPICADW
                     VAALEDSVKHSGADTILVAHSLACLLVAHWAAQTKLPIKAALLVAVPNPKGPTFPAQA
                     IGFDPVPMQPLPFKSTVIASTNDPYGGVAYAEECAAIWGSDLVNIGEAGHINSSSGLG
                     AWPEGHRLLQQLKAD"
     gene            4215..4520
                     /locus_tag="VFF22_08975"
     CDS             4215..4520
                     /locus_tag="VFF22_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZX16978.1"
                     /translation="MPGQYPNRIKQLPLFDGRFDAYKLEAKDSTVLFASYPAGTSIPP
                     HTHDTDNHGVITRGELILSINNQTIRVGVGEWYHVPANVEHSALFEVETDEVEFWFV"
     gene            4568..5119
                     /locus_tag="VFF22_08980"
     CDS             4568..5119
                     /locus_tag="VFF22_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017737885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZX16979.1"
                     /translation="MQAKFRADVLLNRHNAQIIERWDALNLKDGWLVAGCLFQTVWNG
                     LSGEPPESKIKDYDFFYFDPADLSEAAESTVQRHVETVLADLGITVEVANQARVHLWY
                     EAHFGHPYAALSSAREGIDRFLIPGTCVGINPDEVYAPNGLELLYKGTLTPNPLTPHK
                     SLFAEKAASYQQRWPWLCISPDE"
     gene            5272..5889
                     /locus_tag="VFF22_08985"
     CDS             5272..5889
                     /locus_tag="VFF22_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HZX16980.1"
                     /translation="MEYLLAVILFAVSSSVTPGPNNIMVMTSGLNFGIKQSLPLLTGI
                     CVGFTIMLLLVGLGFAQLFDLFPSLHFIIKCVGTLYLLYLAWLIAKSAGAVTAEHQAK
                     PLTFMKGALFQWVNAKAWVVATGAIAAFTTIGASFYTQNLTIALTFFIVSFPCVGVWL
                     LFGSVLKTLLKSEQNRKWFNYSMSALLVLSVVPVVMEIFTQLTHR"
     gene            5939..6247
                     /locus_tag="VFF22_08990"
     CDS             5939..6247
                     /locus_tag="VFF22_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017518450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HZX16981.1"
                     /translation="MITCHIRYVLDPRKLKEFEHYGKLWIALVAKFGGTHHGYFLPSE
                     GANNIGLAMFSFPSLAEYEQYRLKSFADAECLAAFKYAEDTQCIISYERTFFRPVFDA
                     "
     gene            6275..6817
                     /locus_tag="VFF22_08995"
     CDS             6275..6817
                     /locus_tag="VFF22_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017679245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZX16982.1"
                     /translation="MFQLAHFQLMARYNAWMNDKLYAAAGQLSPQALSEDRGAFFPSI
                     LATLNHIAVADIIWLKRFAEHPACAELRAAISDLQRPTALDQLLFDTFAPLHALRGRL
                     DGHISDWVTALTEPDLEHVLAYGNMKGVPAQRPYASLMLHFFNHQTHHRGQASTLLHQ
                     AGQDIGVTDLLALIADVEPR"
BASE COUNT         1820 a         1912 c         1686 g         1449 t
ORIGIN      
        1 cctgcgggcg gcgcggtgag ttgaaacaac ttattaagca cctcaccagc gagaccacac
       61 ggccatcgcc tggtttgttg cagcccggat ttaacacgca gtacctgtag gagccagctt
      121 gctgacgatg ctctgcgacc cggatcgccg ggacgcagga gcgcaacaag ctggcgccta
      181 cgcacaaccg caacagcaac atgtctaccc agcaaggccc gcaagaagaa cgcgccagta
      241 acccactgaa ctgccactct cactgacgga gcacacccta tgcgaaacct gaagaacctc
      301 tgcctgcaaa gcctcggcgt cgccgccctg gcactcggca tgagcagcgc catggcggcg
      361 gacaaaccca ccctgaacat cggctacgta aacggctggg acgacagcgt cgccgccacc
      421 tttgttgccg gggaaatcct caaaagcaac ctcggctaca gtgttgagct caaacccgta
      481 gagccggcca tcatgtggca gggcgtagcc cgtggcgacc tcgacgccac cctctccgcc
      541 tggctgcccg ccacccacgg cgaatacttt gaaaaactca aagacaaggt cgaagtgctt
      601 ggcaccaact acgccggcgc caaaatcggc ctgatcgtgc ctgactacgt acaagccaaa
      661 accatcgccg acctgcaaac ctacgccaaa gacttcgacg gcaaaatcac cggcatcgac
      721 gctggcgccg gcgttatgcg ccgcgccgaa gaagcgatca aggaatacgc cctcaccgac
      781 atcaaactga tgccaagctc cggcccggcc atggccaccg ccctgacccg cgccgagaag
      841 aacaaaaaag ccatcgtcgt caccggctgg atcccacact ggatgttcgc caaatggaac
      901 ctgcgcttcc tcgaagaccc gaaaaaagtc ttcggcgatg acgaacacgt cgacaccgtc
      961 gccaacccca gcctcaacag caaagctcca gaagccgttg ccttcctgaa gaaattctcc
     1021 tggagcggtg aagaagtcgg cgctgtgatg ctcgccatcc gcgaaggcgc caaaccggaa
     1081 gacgccgcca aagcctggat cagcaaaaac ccagagcgtg tcgccgagtg gcttaaataa
     1141 gccaaggctg cacctctata tagaagcggg ctacggcccg cttttttgtg ggcgcgaaat
     1201 gacggcggga gaatgttcta gacaggggga gatgggggtg gatagggttg gggcatgacg
     1261 taatggcgac attcgagcct cataattaat agttaagtac cctttgagag atcgtgatgg
     1321 attctaagaa aaaatgtgga atgtgcagta gtagcgaacc attgcaaaat tcacacataa
     1381 tccctcgctc atattttcga agtcttaaaa atggttgtgg gcagctttac gaggtaagct
     1441 catcagaaaa ggttaaatca aaattaacaa actccgaccc gaaagaggag ctactttgtt
     1501 taaattgtga gcaatttcta tctctaacct acgaaaaata tggaacaaga ttgcttaaag
     1561 atgaaaaaat caagtacgga aaggcatttg taatcttcaa gtcatttagg tacaaggaat
     1621 tttatctata cctaatctcg atattatgga gagcctcaat ttctaaaatc gccaggtatg
     1681 atcacattga tcttgggaat tcaataaaca gtcttctatg ccactgcgtt aagaacaata
     1741 ctataaaaat acaaacatct ctcaggctcg atcacttttt cagaatctct ttaattagaa
     1801 taaaagatga cacgaaaaaa ctggatgata atattcttcg caaagccatg ttcgatatta
     1861 actccgagcc ctctaaaaat cgtgaggacg gtgtaatcta ctatttcatg gttgatggct
     1921 ttctaattat ttatcatatt tcatgtgagg atgacactca taaattgcgc acaaaaaaga
     1981 actatgcaca aatatccaat acaaccaaca tgaaagtgcc agtttcagac attagtaatt
     2041 tcaagcaaat agctgatggg tttaactcga tcaggtcaaa atcaaattag ttcgtatagc
     2101 cgggcactta acaatcgcct gcaatcggac agtcaaacct gccactcgtc tgccataaac
     2161 ccgggaccta ttagagaaca gaacacgatt tcagacagac tgactattca aaaagacaac
     2221 agccaactta tcaccatcaa cggccgagct aaacacaatg aaccgccgca aaaaaatcaa
     2281 acagctacta gaagcacatg ccaaaaaggc caacgccaaa ttggcaccca agaaaaagtc
     2341 cacctatatc agcaaggccg accgcttaaa acttgccgac gagcccaacc aagacttagc
     2401 aatcactcct gaaaactgat acattcttct taagcaccaa ggatggcctg catgtttagc
     2461 cgaataacca ttatcatttt gtgcttactt ctagttgcgt cgaatatttt ctggctatat
     2521 tccgcaattg actctggcgt aactcgcgct tacatgggcc agcaaattta cgaggccaac
     2581 aactcaagtg atcaagcagt aggcatgctc aaaaaactgc tcacgggaaa aaccaaaagc
     2641 gaagttcagg ctctagctca agagttctcg gatatagaag catttgagaa agaaggatgc
     2701 ctgtgggttg gctggtacgg gttcaagttt tcagacgccg acactctaat tgatatagaa
     2761 ccaaagccag gttacagcga aaagtctgca tgtaactctg agctttaaca gcgaattcaa
     2821 actcttcgaa tcgctaagtt aaacggtcta aagcacctta ttggaaatac tatgatcatc
     2881 gtcgtcgaag gcgtcagtgc agccggaaag actacatggg cttcacgaca ctcgccggct
     2941 gttgttagtg aactcaccgg gcatccgccc gacacggaca acgtcacagc cctgggccag
     3001 tactggagtg atcgacacag tgagcgttgg cagcacgggc tagcgctcga acacgtgcac
     3061 aaggttgttt gcttcgacac cgatccattg aaaatccact atgcgtggtg cctttggcag
     3121 atcgggaaag actcgcgcgc ggcttggatg gcggacgtcg aagccagccg cgagcgagtt
     3181 gcacagaaac ggcttggttt cgctgaccag atcgtatttc tcgaaccacc ggaggaagta
     3241 gtccgacagc agaaagccaa cgataaagta cgacgacgca gcaatttcga aacgcatgtg
     3301 cgcctgtacg aaccactgcg gcgctggtac atgctgctgg aaaccttgtc tcccggcagg
     3361 gtggttttca atgcacacca agcaccggac tcaacatcaa tgaccttgca ggcggaccgc
     3421 tacagtctcc agctgtttga tgcgctcatt gatgcagcag accagccgcc caaatagcaa
     3481 aaaactgtgg gaattaaacc aaggcttgct cagcctggcc cacacgaacg ataaagcatg
     3541 acgcaagcca ggcccttagc cacacgctac ctttacgcaa aaggaaactt gccaatatga
     3601 acgtacttat acttccgggt atcggaaact caggaccaga gcactggcaa agccactggg
     3661 aacaggcgaa tagccgctat acccgtgtcc accaagcgga ctgggacaac cccatttgcg
     3721 ctgactgggt agcagcgctc gaagacagcg taaaacactc tggcgcagac accattctgg
     3781 ttgcccatag cctggcatgc ctgctggttg cgcactgggc ggcgcaaacc aagctaccga
     3841 tcaaagcggc cttgctggtc gccgtgccaa acccgaaagg cccaacattc cccgcccagg
     3901 caattgggtt tgatccagta ccaatgcagc ctctgccatt caaatctacg gttatcgcca
     3961 gcaccaacga cccctacggt ggagttgcat acgctgaaga atgtgcagct atttggggca
     4021 gcgatcttgt aaacatcggc gaggccggcc atatcaacag ttctagcggc ttgggcgcct
     4081 ggccagaagg gcacaggctc ctgcagcaac tcaaagccga ctgacaacgt tcaatcttgc
     4141 tcgcctcatt cgtaattaat gggtacagct cgcagcttaa taaaccgtga tggactacaa
     4201 ggaagaacac ttatatgcca ggccaatacc caaaccgaat caaacaactg cccttattcg
     4261 atggccgttt tgacgcctat aagcttgagg caaaagacag caccgttctg tttgcctctt
     4321 accccgccgg cacatccatc ccgccgcata cccacgacac cgataaccat ggcgtgatta
     4381 cgcgcggcga attgatcctg agcataaaca accaaacgat ccgggttggc gtcggcgagt
     4441 ggtatcacgt acctgcaaat gttgagcact cggctttatt tgaggtcgag accgatgagg
     4501 ttgagttctg gtttgtgtaa gacctaacaa ccccgaaatg aacctgccgc cttttgtgta
     4561 gatcaccatg caagcaaagt ttcgcgccga cgtactgctt aaccgccata acgctcagat
     4621 catcgagcgc tgggatgcgt tgaatctcaa ggatggctgg ctagtcgccg gctgcctgtt
     4681 tcagacggtg tggaatgggc tttctggaga gccgccggag agcaagatca aggattacga
     4741 cttcttctac ttcgaccctg ctgacctgag cgaagcggct gaaagcactg tgcagcgcca
     4801 tgtagaaaca gtgctggcag acctgggcat aaccgtggaa gtcgccaacc aggcgcgggt
     4861 gcacctttgg tatgaagcgc attttggcca tccctatgca gcgctgagca gcgccaggga
     4921 aggcatcgac cgctttctga taccgggaac atgcgttggc attaatcccg atgaggtcta
     4981 cgcaccaaac ggccttgagc ttttgtacaa gggaaccctg acaccaaacc cgctgacacc
     5041 gcacaaaagc ctgtttgccg agaaagccgc gtcctatcag caacgctggc cctggctatg
     5101 catcagccca gacgagtagt cacactccaa ccaagcaacc cgcttaaagt aacccactcg
     5161 cttatcaggc tgggtaggtc ccgccctaca gttcaatatc cgaaatagaa aaacttgatt
     5221 tagacccagc agcccccaca tctgctgaaa caaagcgcta tggacaggca catggaatat
     5281 ttactcgcag tcattctctt cgcggtttca tcctcagtaa cacctgggcc aaacaacatt
     5341 atggtgatga catccggcct gaatttcggc atcaagcaaa gcctgccgtt actgacggga
     5401 atatgcgttg gttttaccat catgctgttg cttgtcgggc tagggttcgc ccaactattt
     5461 gatctattcc ccagtttgca cttcatcatc aagtgcgttg gaacgctgta tttactctat
     5521 ctggcctggt taattgccaa atcggccggg gcggttactg cagagcatca agccaaaccc
     5581 ctgaccttta tgaaaggtgc cttgtttcag tgggtgaatg cgaaggcgtg ggtagtcgct
     5641 acaggggcaa ttgcagcctt cacaaccatt ggcgcgagtt tttacacgca gaacctgaca
     5701 atcgccctga ccttttttat cgtgtcgttt ccatgcgttg gggtttggct gcttttcggc
     5761 tcggtactaa aaaccttgct gaagagtgaa caaaatcgaa agtggttcaa ctacagcatg
     5821 tcagcactgc tggttctgtc tgttgtgccc gttgtaatgg aaatattcac tcagctcact
     5881 caccgttgaa cccgtgaacg gcctgaataa gccggcccac accagtagag ggaccaccat
     5941 gatcacctgc catatccgct atgtgctcga cccacgcaag ctcaaggaat tcgagcatta
     6001 cggcaagctg tggattgccc tggtggcgaa gtttggcggc acgcatcacg gttactttct
     6061 gccctcggaa ggtgccaaca atatcggcct ggcgatgttc agcttcccca gcctggcgga
     6121 gtacgagcag tatcggctga aatcctttgc cgacgccgag tgcctggcgg ctttcaaata
     6181 tgcggaagat acccagtgca tcatcagcta cgagcgcacc tttttccgcc cggtattcga
     6241 tgcctgactg aaccttaatt gccgagttaa gcgtatgttc cagctagccc acttccagtt
     6301 gatggcccgc tataacgcgt ggatgaatga caagctctac gcggctgccg gccagctttc
     6361 gccgcaggcg ctgagtgagg atcgcggggc gtttttcccg tccatcctcg ccacgcttaa
     6421 ccatattgca gtggccgata tcatctggct caagcgcttt gccgagcacc cggcctgcgc
     6481 tgagctgcgg gcggcgatca gtgatttaca gcggccgacg gcgctggatc agctgttgtt
     6541 cgacaccttc gcgccgctgc acgctttgcg cggccggctg gatgggcata tcagcgactg
     6601 ggtaacagcg ctgaccgaac cggaccttga gcatgtgctg gcctacggca atatgaaggg
     6661 cgtgccggcg cagcgcccgt acgccagcct gatgctgcat ttcttcaatc atcaaaccca
     6721 ccatcgcggc caggccagca ccttgctgca ccaggccggg caggatatcg gcgtgaccga
     6781 tttgctggcg ctgattgccg acgttgagcc gcgctaatgc gctcaacaac ccgccagagc
     6841 caagacagcc aatgaagcca ctcgccc
//
LOCUS       DASRUM010000132         4388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1414578, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000132 DASRUM010000000
VERSION     DASRUM010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414578"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..760)
                     /gene="tsaA"
                     /locus_tag="VFF22_09000"
     CDS             complement(65..760)
                     /gene="tsaA"
                     /locus_tag="VFF22_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007245428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HZX16983.1"
                     /translation="MPYSVSPIGFVRSCFKEKFAIPRQPQLAPAARGVLELVAPFDNG
                     DAVQGLEQVSHVWLLFLFHQALEDKPRLKVRPPRLGGNQSVGVFSTRATHRPNGIGQS
                     VVKLDKVEADKLWLSGIDLLDGTPVLDIKPYVPYADSVAGAYNRIADSAPALIDVSWQ
                     NSAVIQANEHGQRLNEPLVELIEQCLAQDPRPAYQQPSPERRYGACFWDVDVHWHYPQ
                     PGSICVLEVVPAG"
     gene            complement(800..1570)
                     /locus_tag="VFF22_09005"
     CDS             complement(800..1570)
                     /locus_tag="VFF22_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX16984.1"
                     /translation="MHPYFSLQGRTALVTGGTRGIGKMIAKAFVEAGATVYVCARDGE
                     ACAQTAAELSAFGTCHGIAANLASEEGVQVLAEQLAGTIGSLDILVNNAGTTWGAPLE
                     SYPAKGWEKVMQLNVTSVFSCIQQLLPLLRKAGSAANPARVINIGSVAGITSHGEDAY
                     AYGPSKAALHQLSRMLARELVGQHINVNVIAPGRFPSKMTQFIAQDEAAMAEDCALIP
                     MQRWGREEEMAALAISLASTAGAYMTGAIIPIDGGFHL"
     gene            complement(1691..2152)
                     /locus_tag="VFF22_09010"
     CDS             complement(1691..2152)
                     /locus_tag="VFF22_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010432266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HZX16985.1"
                     /translation="MLNNDVLRSLRYTLDISDAQMVDIIKLSGKQVAPDDLAAMLKKE
                     DEEGFTPCDDELMAHFLDGLVYFKRGKDDSRPAQPFELPITNNMVLKKLRVAFELKED
                     DMHTIMNSVDCPVSKPEMSALFRKFGHSNYRTCGDQFLRNFLKGLTLRVRG"
     gene            complement(2201..2911)
                     /locus_tag="VFF22_09015"
     CDS             complement(2201..2911)
                     /locus_tag="VFF22_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016702025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HZX16986.1"
                     /translation="MTDPIRLSKRLIEVIGCSRREAELYIEGGWVTVDGVVVEEPQFK
                     VAEQRVELLPNAVLTPQEPVTLLLHIPAGHYPDRAVESIGADGHWAEDAAGIRPLKSH
                     FARLVSCAPLQAGADGLHVLTQDWRIERKLKEDLSKLEQEYVVEVVGTLSASQLKRLA
                     HGLTFNGVALAPCKVSWQNETRLRFALKNPLPGQIVFMCNSVDLKVLGMKRIRIGGVP
                     MAKVPAGQWRYLAGKERF"
     gene            complement(3021..3218)
                     /locus_tag="VFF22_09020"
     CDS             complement(3021..3218)
                     /locus_tag="VFF22_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX16987.1"
                     /translation="MNKTPRAVAQQSLIQASVFALAAIALIVLWLAAEVFLVVFAGIL
                     LALLFHGAGQLISDHSLPAER"
     gene            complement(3535..4236)
                     /locus_tag="VFF22_09025"
     CDS             complement(3535..4236)
                     /locus_tag="VFF22_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX16988.1"
                     /translation="MLYFDKVSTFYGKIQALHDVSVEVRQGEIVTLIGANGAGKSTLL
                     MTLCGSPQASSGSIRYQGEELIGQDTPSIMRKSIAVVPEGRRVFSRLTVEENLVMGGF
                     FGSKADNQEQLDKVLHLFPRLKERFAQRAGTMSGGEQQMLAIGRALMSKPKLLLLDEP
                     SLGLAPIIIQQIFDIIEQLRKDGVTVFLVEQNANQALKLADRGYVLENGRIVMQGSGH
                     DLLNDPKVRDAYLGG"
     gene            complement(4236..>4388)
                     /gene="livG"
                     /locus_tag="VFF22_09030"
     CDS             complement(4236..>4388)
                     /gene="livG"
                     /locus_tag="VFF22_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249762.1"
                     /note="Part of the ABC transporter complexes LivFGHMJ and
                     LivFGHMK involved in the high-affinity transport of
                     branched-chain amino acids; LivFGHMK is specific for the
                     transport of leucine, while LivFGHMJ is a transporter for
                     leucine, isoleucine, and valine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HZX16989.1"
                     /translation="TVLLIEHDMKLVMSISDHIYVINQGTPLADGTPEQIRANPDVIK
                     AYLWEA"
BASE COUNT          843 a         1269 c         1384 g          892 t
ORIGIN      
        1 catttatctg gtaaatataa gacaaaataa ctgatcagcc tatgtgtggg gggtacgaac
       61 tagatcagcc ggccggtacg acttccagca cgcagatact gcctggctgc gggtagtgcc
      121 agtgcacatc gacatcccag aagcacgcgc catagcggcg ttctgggctg ggctgctggt
      181 aggcaggtcg cgggtcctgg gccaggcatt gttcgatcaa ctcgaccagt ggttcattca
      241 ggcgctgccc atgttcgttt gcctggatca ctgcactgtt ctgccagctg acgtcgatca
      301 gcgccggcgc gctgtcggcg atgcggttgt aggctccggc gacgctgtcg gcatagggca
      361 cgtagggttt gatatccagc accggcgtgc cgtcgagcag gtctataccg gacagccaca
      421 gtttgtcggc ttcaacctta tccagcttaa ccaccgactg gccgatgcca ttgggccggt
      481 gggtggctcg ggtgctgaat acgccgaccg actgattgcc gcccaggcgc ggtgggcgca
      541 ccttgagccg cggcttgtct tccagcgcct ggtgaaacag aaacagcagc cagacatggc
      601 tgacctgctc caatccctgc accgcatcgc cgttatcgaa cggcgcgaca agctccaata
      661 cgccacgggc cgccggtgcc aactgcggct gacggggaat ggcgaacttc tccttgaagc
      721 aggagcggac aaaacctatg ggggaaacgg agtagggcat gacggcgtga tcgagtgaaa
      781 acgccgccat agtacctgac tagagatgaa aaccgccgtc gatggggatg atcgcgccgg
      841 tcatataagc cccagccgtg ctggccaggc tgatcgccag ggcggccatc tcctcttcgc
      901 ggccccagcg ctgcatcggg atcagggcgc agtcttcggc catggccgct tcgtcctggg
      961 cgataaactg ggtcatcttg ctcgggaagc gtcccggagc gattacgttg acgttgatat
     1021 gttggccgac cagctcgcga gcgagcatgc gcgacagctg atgcagcgcc gccttgctcg
     1081 ggccgtaggc ataagcgtct tcgccgtggg aggtgatgcc ggcgaccgag ccgatgttga
     1141 tcacccgtgc cggattagcc gcgctgcctg ccttgcgcag tagcggtagc aattgctgga
     1201 tgcagctgaa caccgaggtc acattgagct gcatgacctt ttcccagccc ttggccggat
     1261 agctctccag tggggcgccc caggtggtac cggcgttatt gaccaggata tccaggctgc
     1321 cgatcgtgcc ggccaattgc tcggccagca cctgcacacc ttcctcgctg gctaggttgg
     1381 cggcgatgcc gtggcaggtg ccaaaggctg atagctcggc agcggtttgt gcgcaggctt
     1441 cgccgtcacg ggcgcagaca tagactgtgg cgccagcttc gacaaaggct ttggcgatca
     1501 tcttgccgat gccgcgggtg ccaccggtaa ccagggcggt gcggccttgc agactgaaat
     1561 aggggtgcat ggcgaatcct tttaacggat gagtgccact accctaatca tctgactgca
     1621 ccatggcagt catcattgcc cgtggaaata cgctgccatg cgggcaagca tggcagcgag
     1681 gtgctgggac ttagccgcga acgcgcaggg tcagaccctt aaggaagttg cgcagaaact
     1741 gatcgccgca ggtgcggtag ttgctgtggc cgaacttgcg gaacagcgcg ctcatttccg
     1801 gcttggaaac cgggcagtcg acgctgttca tgatcgtgtg catgtcgtct tccttcagct
     1861 cgaaggccac gcgtagtttc ttcagtacca tgttgttggt gatcggcagc tcaaacggct
     1921 gagccgggcg gctgtcgtcc ttgccgcgct tgaagtacac caggccgtcg agaaagtgcg
     1981 ccatcaactc gtcgtcgcag ggcgtaaagc cttcttcgtc ttccttcttg agcattgccg
     2041 cgaggtcgtc gggggcgacc tgcttgccgc tgagcttgat gatgtcgacc atctgcgcgt
     2101 cgctgatgtc gagggtgtag cgcaggctgc gcagtacgtc gttattaagc atggggtggt
     2161 cctgaaaagg gctgatgcac aagacacatc agctgaaaaa ttagaagcgt tctttgcctg
     2221 ccaggtagcg ccactggccc gcagggacct tggccatcgg cacgccgccg atgcggatgc
     2281 gcttcatgcc cagcaccttg aggtcgacgc tgttgcacat aaacacgatc tgcccaggca
     2341 gtgggttctt caaggcgaag cgcaggcggg tttcgttttg ccagctgacc ttgcatgggg
     2401 ccagggccac accgttgaag gtcaggccgt gagccaggcg cttgagttgg ctggcgctca
     2461 acgtgccgac gacttcgacc acatattcct gctccagttt gctcaggtct tccttgagtt
     2521 tgcgctcgat gcgccagtcc tgggtcagta catgcaggcc atcggccccg gcttgcagag
     2581 gtgcgcagct gaccaggcga gcgaaatggc ttttcagcgg gcggatgccg gctgcgtcct
     2641 cagcccagtg gccgtccgcg ccgatactct cgacggcgcg atctgggtag tgcccggcag
     2701 gtatgtgcag cagcagggtg accggctcct gcggggtcag tacggcattt ggaagcagct
     2761 cgacacgctg ctcggcaacc ttgaactgcg gctcttccac caccacacca tccaccgtga
     2821 cccagccgcc ttcgatatag agttcggcct cgcggcgcga acagccgatc acttcgatca
     2881 gacgtttgga caggcgaatg gggtcggtca tggaagcggt catcggtaaa aaagggcggc
     2941 cattgtaact ggcctgaagg tttgtgcgcg ggccaatggg cgaacggcgg ggaaaatcta
     3001 gtgttcaggg caggggagcg ctatctctcg gcaggaaggg agtggtcgct gatcaattga
     3061 cctgcgccat gaaagagcaa tgccagcagg atgccggcaa ataccaccag aaatacctcg
     3121 gcggccagcc acagcacgat cagggcgatg gccgccaagg caaatacgct ggcctgaatc
     3181 aagctctgtt gggcgactgc ccgaggcgtc ttattcatca agcaggtttt gttgtgagta
     3241 gcgcatcagc acaaggtcct ttggctggcc tgaaccgcgc aaattagaac accagcataa
     3301 acaggccgat cacgaccagc aggccgataa ggaagatgat gccgatggtg cttccgagaa
     3361 atttcagcat ggaaagctcc tgtatatcgt tgtgggctct ctattaccga ctgactcggt
     3421 tactcatcgt tcagacaatt taaggcgcgc gctttctgca agcagaaggg aatggattcg
     3481 gggtgtgagg cagagtgaaa tgaaaagcgg ccccgaaggg ccgctggtca agggttagcc
     3541 gcctaagtag gcgtcgcgta cctttggatc gttcagcagg tcgtgcccgc tgccttgcat
     3601 gacgatccgg ccgttctcca gtacatagcc gcggtcggcc agcttgagcg cctggttggc
     3661 gttctgctcg accaggaaca ccgtcacacc gtccttgcgc agctgttcga tgatgtcgaa
     3721 gatctgctgg atgatgatcg gtgccaggcc cagcgacggc tcatcgagca gcagcagctt
     3781 gggcttgctc atcagcgcac ggccgatggc gagcatctgc tgctcaccgc cggacatggt
     3841 gccggcgcgc tgggcaaagc gttccttcag gcgcgggaac aggtgcagga ccttgtccag
     3901 ctgttcctga ttgtcggcct tgctgccgaa gaagccgccc atcaccaggt tttcttccac
     3961 ggtcaggcgg gagaacaccc ggcgaccttc cggcaccacg gcaatgctct tgcgcatgat
     4021 gctcggggtg tcctggccga tcaactcttc accctgatag cggatgctgc cactgctcgc
     4081 ctgcggtgag ccgcacaggg tcatcagcag ggtcgatttg ccggcgccgt tggccccgat
     4141 cagggtgacg atctcaccct gacggacctc gacgctgacg tcgtggagag cctggatctt
     4201 gccgtagaag gtggaaacct tgtcgaaata cagcatcaag cttcccacag gtaggctttg
     4261 atcacgtccg ggttggcgcg gatctgctcc ggcgtgccgt ccgccagggg cgtgccctgg
     4321 ttgatcacgt agatgtggtc ggaaatgctc atgaccagtt tcatgtcgtg ctcaatcagc
     4381 agcacggt
//
LOCUS       DASRUM010000133         6734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_73250, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000133 DASRUM010000000
VERSION     DASRUM010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6734
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73250"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1837
                     /gene="rpoD"
                     /locus_tag="VFF22_09035"
     CDS             <1..1837
                     /gene="rpoD"
                     /locus_tag="VFF22_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HZX16990.1"
                     /translation="AQQQSRIKELITLGREQKYLTYAEVNDHLPEDISDPEQVEDIIR
                     MINDMGIPVHESAPDADALMLADADTDEAAAEEAAAALAAVETDIGRTTDPVRMYMRE
                     MGTVELLTREGEIEIAKRIEEGIREVMGAIAHFPGTVDSILAEYTRVTTDGGRLVEVL
                     SGYIDPDDGIVPAEAAAPVPAKDGDAADDKEDEEDGDASDDEEEEGDGGPDPEEALRR
                     FTAIGDALDLAKKALKKHGRGSKQGIAALKEMAELFMPIKLVPKQYDALVVRVRSSLE
                     RLRAQERAIMQLCVRDARMPRADFLRLFPGNEIDVEWSAGLAKGKAKYAEAIGNLQGD
                     IQRCQQKLSALEAECELTLAEIKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKY
                     TNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRI
                     PVHMIETINKLNRISRQMLQEMGREPTPEELGERMEMPEDKIRKVLKIAKEPISMETP
                     IGDDEDSHLGDFIEDSTMQSPIDVATVESLKEATREVLSGLTAREAKVLRMRFGIDMN
                     TDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPTRSEHLRSFLDE"
     gene            1942..5676
                     /locus_tag="VFF22_09040"
     CDS             1942..5676
                     /locus_tag="VFF22_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZX16991.1"
                     /translation="MPRVAATLLLCLACWALPAFSLELTPAEQTWLAEHPQLSLGVDR
                     DWPPYEFIDDNDLYQGLAADYVRLIEQRLSITLRPAPAQNWRQVLADARTGKLNLLPS
                     LMATPEREQYLTFTRPYLDFPIVILTQEKGPQPRKIRELQGLRVAVVDSYATHELLRD
                     KHPELRLWPRPSVAAALQALASGQADAMVGDLASSVWHLRQLKLDGIVISGQTPYRYQ
                     LAMAVPKDQAILAGILDKLLDELTPSEIAEIQQRWVGEPIDQRPFWRRLLLFGLPGLL
                     AALLIIFSILRINHRLRSEMHNRALLEGELRNSEQHYRGLVESLNAVAWQMDPSDFRY
                     TYVAPQAERLLGYPPQDWLQPGFIERILHPDDAHRTLDYCRSETQAGRDHSMDYRMLA
                     ADGREVWVRDIVTLSPLDGSQMLRGLLIDITETKRTEQALALSEQKFASVFHNCPDII
                     VLLNLHDGRLLAVNNTFEQQFGISAAEAIGHTSSELDLWIEQGIGPRILEMLLKGNAH
                     NIEGTFRRRDGSQFTALISAQKVTLNDSSTLVVAVRDISELKDTQQRLKLSEDKFAKA
                     FHASPDGLLITRLSDGQLLDVNEGFSRITGYSVNEATDSSTLQLGIWANPNDRIRMFE
                     HVQQYGSVRDFRALIRTRNGSLRTCEMSVQPIPIEGDTCMLTIARDITEREQMQENLK
                     QAATVFESTAEGVMITDLQQRITAVNRAFTSITGYSEAEALGQSPRLLASGNHDSAFY
                     AAMWHNLSASGHWQGEIWNKRKNGELYPEWLTISAVRDSDQHITHFVGVFADISSLKH
                     AQASLDHQAHHDPLTGLPNRMLFEARLRAALEDAHIDKRMGAVLFIDLDRFKHINDSL
                     GHPVGDQLLKSIAERLKSHLRDIDTVARLGGDEFIILLPGLQHDDDAELVAHKLLDCF
                     SLPFQFDEQELFISASIGISRYPEDGDDVATLVKNADAAMYRSKARGRNRAELYTRDL
                     TFQATERMALERELRRAIELEQLELYYQPKRSLSNNRLIGAEALLRWHHPVFGEIPPD
                     RFIPLAEETGLIIALGDWVLRQACWQMQQWQQQHAPFGPLSVNLTGVQLRQPHLLVRI
                     SSLLENHNLAPELLQLEITESFIMNQAEEALSLLHQLKSLGIQLAIDDFGTGYSSLSY
                     LKRLPVDTLKIDQSFVRGLPNDSNDVAIVRAIIALGRSMQLTVIAEGVETKAQELFLA
                     AEGCEQIQGFVISRAIHADAFAQNFLTPRHSVGAAEKAPV"
     gene            5692..5768
                     /locus_tag="VFF22_09045"
     tRNA            5692..5768
                     /locus_tag="VFF22_09045"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5726..5728,aa:Ile,seq:cat)
     gene            6489..>6734
                     /locus_tag="VFF22_09050"
     CDS             6489..>6734
                     /locus_tag="VFF22_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZX16992.1"
                     /translation="MTSLYDVSEMLKQARGDAKLSQEALASSAGVSRSTVARMETLAK
                     GDMSVSVLVRLLEAAGYDLKLVKAGHERTVEDILNEQR"
BASE COUNT         1526 a         2192 c         1836 g         1180 t
ORIGIN      
        1 agcgcaacag cagtctcgta tcaaagagtt gatcaccctg ggtcgtgagc agaagtatct
       61 gacttacgca gaggtcaacg accacctacc ggaagatatt tctgatccgg agcaggtgga
      121 agacatcatc cgcatgatca atgacatggg gatccccgta cacgagagtg ctccggatgc
      181 ggacgccctt atgttggccg acgccgatac cgacgaggca gcagctgaag aagccgctgc
      241 agcgctggca gcggtggaga ccgatatcgg ccgcacgacg gacccagtgc gcatgtacat
      301 gcgtgaaatg ggcaccgtgg aattgctgac ccgcgaaggc gagatcgaaa tcgccaaacg
      361 catcgaggaa ggcatccgtg aagtcatggg cgctatcgcc catttccccg gcactgtcga
      421 cagcattctg gccgagtaca cccgtgttac caccgatggt ggtcgcctgg ttgaagttct
      481 cagcggctat atcgatccgg atgacggcat cgttcctgcc gaagccgccg cccccgtgcc
      541 tgcaaaggac ggcgacgcag ccgacgacaa ggaagatgaa gaagacggcg acgccagcga
      601 cgacgaggaa gaggaaggcg acggcggtcc ggatcctgaa gaggccctgc gccgcttcac
      661 cgcgattggt gacgctcttg acctcgccaa gaaagccctg aaaaagcacg gccgtggcag
      721 taagcaaggc attgccgcgc tgaaagaaat ggccgagctg tttatgccga tcaagctcgt
      781 gcctaagcag tacgacgccc tggttgtgcg tgtacgcagc tccctggagc gcctgcgcgc
      841 ccaggaacgc gccatcatgc aactctgcgt acgtgatgca cgtatgccgc gcgccgactt
      901 cctgcgcctg ttcccaggca atgaaatcga cgtcgagtgg tcggctggcc tggccaaagg
      961 taaggccaag tacgccgaag ccatcggcaa cctgcaaggc gatatccagc gctgccagca
     1021 aaagctgtct gccctggaag ccgagtgcga gctgaccctg gctgaaatca aggacatcaa
     1081 ccgtcgcatg tcgatcggcg aggccaaggc ccgtcgcgcg aagaaagaga tggttgaagc
     1141 gaacctgcgt ctggtgatct cgattgccaa gaagtacacc aaccgcggcc tacaattcct
     1201 tgacctgatc caggaaggca acattggcct gatgaaggcg gtggacaagt tcgaataccg
     1261 tcgcggttac aagttctcga cctatgcaac ctggtggatc cgtcaggcga tcactcgctc
     1321 gatcgccgac caggcgcgca ccatccgtat tccggtgcac atgatcgaga cgatcaacaa
     1381 gctcaaccgt atttcccggc agatgctgca ggaaatgggt cgcgaaccga ccccggaaga
     1441 gctgggcgaa cgcatggaaa tgcctgagga caagatccgc aaggtattga agatcgctaa
     1501 agagccgatc tccatggaaa ccccgatcgg tgatgacgaa gactcccatc tgggtgactt
     1561 catcgaagac tcgaccatgc agtcgccaat cgatgtcgcc accgttgaga gccttaaaga
     1621 agcgactcgc gaagtcctct ccggcctcac cgcacgtgaa gccaaggttc tgcgcatgcg
     1681 tttcggcatc gatatgaata ccgaccacac ccttgaggaa gttggtaagc agttcgatgt
     1741 aacccgcgag cggattcgtc agatcgaagc caaggcgctg cgcaagctgc gccacccgac
     1801 gagaagcgag catctgcgct cattcctcga cgagtaacac caaaaccccc ggccctgccg
     1861 ggggttttgc attctgggca actaccaagg cctgccaggc ggtctacact ttccctaata
     1921 ccctagcgaa cgagcccacc catgccgcgt gttgcagcca ccctcctgtt gtgtctggcg
     1981 tgctgggcct tgcccgcgtt cagccttgag ctgacgcccg ccgaacaaac ctggctggcc
     2041 gaacatccgc aactgagcct gggcgtggac cgagactggc ccccttacga attcatcgat
     2101 gacaacgacc tctaccaggg cctcgccgcc gactatgtgc gcctgattga gcagcgccta
     2161 tcaatcaccc tacgcccggc tcccgctcag aactggcgcc aagtactggc cgatgccaga
     2221 accggcaagc tgaacctact gcccagcctg atggccaccc ccgagcgtga gcagtacctg
     2281 accttcaccc gcccctacct cgacttcccg attgttatcc tgacccagga aaaaggccca
     2341 caaccgcgca agatacggga gttgcagggg ctcagagtcg ccgtggtcga cagctacgcc
     2401 acccacgaat tgctgcgcga caaacacccc gagctgcgtc tctggccgcg ccccagtgtg
     2461 gcagccgcct tgcaagcact ggcctccggg caagccgatg cgatggtcgg cgacctcgcc
     2521 tccagtgtct ggcatctgcg ccaactcaag ctcgatggca tcgtcatcag cggccagact
     2581 ccctatcgct accaactggc catggccgta cccaaggacc aagccatcct ggccggtatt
     2641 ctcgacaagc tgctggacga actcaccccg agtgaaatcg ctgaaatcca gcaacgctgg
     2701 gtgggcgagc cgattgacca acggccattc tggcgccgcc tgctgctgtt tggcctgccc
     2761 ggcctgctag cggccttgct gattatcttc agcatcctgc gcatcaacca ccgcctgcgc
     2821 agtgaaatgc ataaccgcgc cttgctcgaa ggcgagctgc gcaacagcga gcagcactac
     2881 cgtggcctgg tggaaagcct caacgccgtg gcctggcaga tggaccccag cgatttccgc
     2941 tacacctatg tcgcgcccca ggcggaacgc ctgcttggtt atccgcccca ggactggctg
     3001 caacctggct ttatcgaacg tatcctgcac cccgatgacg cgcaccgcac actcgattat
     3061 tgccgcagtg aaacccaggc cgggcgcgac cacagcatgg actaccgcat gctcgccgcc
     3121 gacggccgcg aagtctgggt acgcgatatc gtcacgctgt cgccgctgga tggcagtcag
     3181 atgctgcgag gcctgttgat cgacatcacc gaaaccaagc gcaccgaaca ggcgctggcc
     3241 ctgtcggaac agaaattcgc ctcggtgttc cacaactgcc cagacatcat cgtcctgctc
     3301 aacctgcatg acggccgcct actagcggtc aacaacacct tcgagcaaca gttcggcatc
     3361 agcgccgccg aagccatagg gcacaccagc tcggagctgg acctgtggat cgagcaaggc
     3421 atcggcccgc gcattctgga aatgctgctc aagggtaacg cccacaatat cgagggcacc
     3481 ttccgccggc gcgatggcag ccagttcacc gcactgattt ccgcgcagaa ggtcacccta
     3541 aatgacagca gtacgctggt cgtggcggta cgtgacatca gcgagctgaa agacacccag
     3601 cagcgcctga aactgtccga ggacaagttc gccaaagcct tccatgcctc ccccgacggc
     3661 ctgctgatta cccgcctgag cgacggtcag ctgctagatg tcaacgaagg cttcagccgc
     3721 atcaccggct acagcgtcaa cgaagcgacc gacagttcga ccctgcaact gggcatctgg
     3781 gctaacccga acgaccgcat ccggatgttc gaacatgtgc agcaatacgg ctcagtgcgt
     3841 gatttccgcg cccttattcg cacccgcaac ggctcgctgc gtacctgcga gatgtcggtg
     3901 cagccgatcc ccatcgaagg cgacacctgc atgctgacca tcgcgcgcga catcaccgag
     3961 cgcgagcaga tgcaggaaaa cctcaagcag gccgccaccg ttttcgaaag caccgctgaa
     4021 ggcgtgatga tcaccgacct gcagcagcgc atcacagcgg tcaatcgcgc cttcaccagc
     4081 atcaccggct acagcgaagc cgaagccctc ggccaatcgc cgcgcctgct cgcctccggc
     4141 aaccacgaca gcgcgttcta cgccgccatg tggcacaacc tcagcgcttc cgggcactgg
     4201 caaggcgaga tctggaacaa acgcaaaaac ggcgagctct acccggaatg gctgaccatc
     4261 agcgccgtgc gcgacagcga tcagcacatc acccactttg tcggcgtgtt cgccgatatc
     4321 agctcgctca agcacgccca ggccagcctc gaccaccaag cccaccacga cccgcttacc
     4381 ggcctaccca atcgcatgct gttcgaagcc cgcctgcgcg cggccctcga agacgcgcac
     4441 atcgacaagc gcatgggcgc cgtactgttt atcgacctgg accgcttcaa acacatcaac
     4501 gacagcctcg gccatccagt cggcgaccaa ctgctcaaga gcattgccga acgcctgaag
     4561 tcccacctgc gtgatatcga caccgtggcc cgcctcggtg gtgatgaatt catcatcctg
     4621 ctgccaggcc tgcagcacga tgacgatgcc gaactggtgg cgcataaact gctcgactgc
     4681 ttcagcctgc ccttccagtt cgacgagcaa gagctgttta tcagcgccag cattggcatc
     4741 agccgctatc cggaggacgg tgacgatgtc gccaccctgg tgaaaaacgc cgacgccgcc
     4801 atgtatcgct ccaaagcgcg cgggcgcaac cgcgcagagc tgtacacccg tgacctgacc
     4861 ttccaggcca ccgaacgcat ggccctggaa cgcgaactgc gccgcgccat cgaactggaa
     4921 cagctagagc tctactacca acccaaacgc agcctaagca ataaccgtct gatcggcgcc
     4981 gaagcgttgc tacgctggca tcacccggta ttcggcgaaa tcccgccaga tcgctttatt
     5041 cccctggccg aagaaaccgg cctgatcatc gccctcggcg attgggtgct gcgccaagcc
     5101 tgctggcaaa tgcagcaatg gcaacaacag cacgccccgt tcggcccact atcggtcaat
     5161 ctgaccggcg tgcaactgcg ccagccgcac ctgctggtac gcatctccag cctgctggaa
     5221 aaccacaacc tggccccaga attgctgcaa ctggaaatca ccgaaagctt catcatgaac
     5281 caggccgaag aagccctgag cctgctacat cagctgaaaa gcctgggcat ccaactggcc
     5341 atcgacgact tcggcaccgg ctactcctca cttagctacc tcaagcgcct gccggtcgac
     5401 accctgaaaa tcgaccaatc cttcgtgcgc ggcctgccca atgacagcaa cgacgtcgcc
     5461 atcgtgcgcg ccatcatcgc cctgggccgc agcatgcaac taaccgtgat cgccgaaggg
     5521 gtggaaacca aagcccagga actgttcctc gccgccgaag ggtgcgagca gatccagggc
     5581 ttcgtcatca gccgcgccat ccatgcggac gccttcgcac aaaacttcct caccccgcgc
     5641 cattcggttg gcgctgctga gaaggcaccg gtataatccg cccgcctctg agggcccata
     5701 gctcagttgg ttagagcaga ggactcataa tcctttggtc cacggttcga gtccgtgtgg
     5761 gcccaccata aaaaacaaag agttaggcca gccttcgagc tggccttttt cttttctgcg
     5821 ctaaaaatta ttttttggtc accgtattgg tcaccacttg aaacttactg cagcctgacc
     5881 ttgcccaact agccgtgctg ctatgccaat acaaatgagc tgaagggaaa gacggctacc
     5941 caagtcttgc tccaaccacg gacgctccgg ctaccagctg gattggagtg gtgtttggtc
     6001 gactcaagtg gaagctttca tgcagtgaat atcgtggagc tggtagacgg cgttgcggta
     6061 aggggctacg gcaaccaaat cagcctgccc gaagacacca gccactcgtg atctacccat
     6121 aatttctgtg gtgagaaccg ccgccgagct tagagcaagc cgagtctaca accttgtggt
     6181 caccaaataa cgaaccgcgc ttaacaccag agccccaagc acggggcgat tcagagctgc
     6241 ccggtaagca gactgtcctc agtccctgga ctgaggacag agctcgcctc accccactcc
     6301 aaccctaggg cgcagccatt ggcctcgcca tgccagctgg tttgtggcct tgaagccaca
     6361 aataatcact aacacgtgga ttttgtagcc cgtgagccac agctcaagcc tggcagcact
     6421 tgtataaatg tagccccaga gctacatttt taggcattag atctattttt gacgcccgag
     6481 ggctacaaat gacctctctc tacgatgttt ccgaaatgct gaaacaggca cgcggtgatg
     6541 ccaagctgag ccaagaggca ttggccagca gcgcaggggt atcacgctcc acggttgcgc
     6601 gcatggaaac attggccaaa ggcgatatga gtgtctcggt gctggttcgg ctgctggaag
     6661 cggcaggcta tgacttgaag ctggttaagg ccgggcacga acgcacggtt gaagacatcc
     6721 tcaacgagca gcgg
//
LOCUS       DASRUM010000134         3700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_400244, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000134 DASRUM010000000
VERSION     DASRUM010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400244"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1825)
                     /gene="ngg"
                     /locus_tag="VFF22_09055"
     CDS             complement(89..1825)
                     /gene="ngg"
                     /locus_tag="VFF22_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine synthetase"
                     /protein_id="HZX16993.1"
                     /translation="MRATAFNQRLLRGQAPSYERLQARLAEDHNTPPSEPQAVHCGWG
                     RLLLGHTYPDAAALASDLLTEQPGERDIALYVAAPHQVLAQAPQQLFLDPSDTLRLWF
                     TDYRPARRGFRGYRVRRAQNAEDWQAINELYQLRGMLPIEPGKLTPRHLGGPVYWLAE
                     DEDSGAVIGSVMGLNHHKAYQDPDHGSSLWCLAVDPRCSRPGVGEVLVRHLIEHFMSR
                     ELSYLDLSVLHDNEQAKGLYAKLGFRELQTFSIKRKNGINEALFLGPGPHTPMNPYAR
                     IIVDEALRRGIEVQVDDADAGLFTLSHGGRRIRCRESLTDLTSAVSMTLCQDKRLTHR
                     ALARAGLHLPAQRLAGSAEDNAAFLAEHGSLVVKPVDGEQGQGVAVDLRTPLDVQDAI
                     ERASTFDSRVLLESFHPGFDLRILVIGFAVVAAAIRRPAEVIGDGQHSIGELIDAQSR
                     RRQAATEGESRIPKDAETLRTLHAAGVDYDSVLPAGQRLAVRKTANLHTGGSLEDVTE
                     ILHPVLAEAAVKAARALDIPVVGLDLLVPAADQPEYVFIEANERAGLANHQPQPTAER
                     FVDLLFPLSQVG"
     gene            complement(1900..3669)
                     /locus_tag="VFF22_09060"
     CDS             complement(1900..3669)
                     /locus_tag="VFF22_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine amidotransferase"
                     /protein_id="HZX16994.1"
                     /translation="MCAIAGELRFDQRPADLAAVERITHHMAARGPDAHGFFSQGPLA
                     LGHRRLKIMDLAEASGQPMIDSELGLTMVFNGAIYNYPELREELKALGYRFFSGGDTE
                     VLLKGYHAWGTDLLPKLNGMFAFAIWERDSQQLFIARDRLGVKPLYLSRTGERLRFAS
                     SLPALLAGGDISKTLDPVALNHYLNFHAVVPAPRTLLAGVEKLAPASWMRIDAQGNCE
                     QKTWWNLTFGPQREEIAFGLDEWQERVLDGMREAVSIRQRAAVDVGVLLSGGVDSSML
                     VGLLREAGVENLSTFSIGFQDAGGERGDEFQYSDLIAEHFATRHHQLRIGEHEILEQL
                     PAAFRAMSEPMVSHDCIAFYLLSREVAKHCKVVQSGQGADELFAGYHWYPQVDAAEDA
                     FGAYRAAFFDRDYDEYAACVQPAWLTGDMAGEFVREHFAQPGAVAAVDKALRLDSTVM
                     LVDDPVKRVDNMTMAWGLEARTPFLDYRLAELSARIPGRFKLPDGGKHVLKEAARQVI
                     PSAVIDRPKGYFPVPGLKHLQGATLGWVRDLLLDPSQDRGLFNPAMLDRLLSDPHGQL
                     TPLRGSKLWQLAALNLWLNEHGL"
BASE COUNT          623 a         1132 c         1275 g          670 t
ORIGIN      
        1 taagcggcgt agcccgaata agccttggcg caatccgggg gcgttttcct ccggctgtgg
       61 cctgtgccgc ggcttatccg gggcggcttc aacctacctg gcttagggga aacaataggt
      121 cgacgaagcg ctctgccgtg ggttgcggct ggtggttggc caggccggcg cgctcgttgg
      181 cttcgataaa cacgtactcg ggttgatcgg cagcgggcac cagcaggtcg aggccgacca
      241 ccgggatatc cagagcgcgg gcggctttta ccgccgcttc ggccagcacc gggtggagga
      301 tctcggtaac gtcctccagg ctgccgccag tgtgtaggtt ggcggttttg cgcacggcca
      361 ggcgctggcc ggccggcagc acgctgtcgt agtcaacccc tgcggcgtgc agggtgcgca
      421 gggtttcagc gtccttgggg atgcggcttt cgccttcggt ggcggcctgc cggcggcggc
      481 tttgcgcatc gatcagctca ccaatactgt gctggccatc gccgattacc tcggccgggc
      541 ggcgaatggc ggccgcgacc acggcaaagc cgatcaccag aatgcgcaga tcaaagcctg
      601 ggtgaaagct ttccagcaag acgcggctgt caaaggtgct ggcgcgttcg atggcatcct
      661 gcacatccag cggcgtacgc aggtccaccg ccacgccctg gccctgttcg ccatccaccg
      721 gcttgaccac caggctgcca tgctcggcga gaaacgcggc gttgtcctcg gcgctgccgg
      781 ccaggcgttg ggctggcaag tgcaagccgg cgcgggccag ggcgcgatgg gtcaggcgct
      841 tgtcctggca gagggtcatg ctgactgcgc tggtcaggtc tgtcagcgat tcccggcagc
      901 ggatgcgccg gccgccgtgg cttaaggtaa acaggccggc gtcggcgtca tcgacctgta
      961 cctcgatgcc gcggcgcagc gcttcatcga cgatgatccg cgcgtagggg ttcatcggcg
     1021 tgtgcgggcc ggggccgagg aacagcgctt cgttgatgcc gtttttgcgt ttgatgctga
     1081 aggtctgcag ctcgcgaaag cccagcttgg cgtacagccc cttggcctgt tcgttgtcat
     1141 gtagcacgga cagatccaaa tagctgagct cgcggctcat aaagtgctcg atcagatggc
     1201 gcaccagcac ctcaccgacc cccggccggc tgcagcgcgg gtcgaccgcc aggcaccaca
     1261 gactgctgcc gtggtccggg tcctgatagg ccttgtggtg gttaaggccc atgacactgc
     1321 cgatcaccgc gccgctgtct tcgtcctcgg ccagccaata caccggcccg ccaaggtggc
     1381 gtggagtgag tttgccgggt tcgatcggca gcatgccgcg cagttgatac agctcattga
     1441 tcgcctgcca gtcctcggca ttctgcgcgc ggcgcactcg gtaaccacga aaaccgcggc
     1501 gcgcagggcg gtagtcggta aaccacagac gcagcgtgtc ggaggggtcg agaaacagct
     1561 gctgtggggc ctgcgccagc acttggtgcg gcgcggcgac atacagggcg atatcgcgct
     1621 cgccgggctg ttcggtcagt aggtcactgg ccagggcggc ggcatccggg taggtgtgcc
     1681 cgagtagcag gcggccccag ccgcagtgca cggcttgcgg ctcgctggga ggggtgttgt
     1741 ggtcctcggc caggcgcgct tgcaggcgct cgtaggaggg cgcttggccg cgcagcaggc
     1801 gttggttaaa ggcagttgca cgcattggag ggtttccttt gccgtgagca ggttgcggat
     1861 gctgcggtgg atgcgtctgt gcccagccac cgcagcgctc tacaggccgt gttcgttcaa
     1921 ccagagattc agcgcagcca gctgccacaa cttggagccg cgcaagggcg tcagctggcc
     1981 gtgcgggtca ctcagcagac ggtcgagcat ggccgggttg aacaggccgc gatcctggct
     2041 cgggtcaagt agtagatcgc gcacccagcc cagagttgca ccttgcagat gcttgaggcc
     2101 aggcaccggg aagtaaccct tgggccggtc gatcaccgcg ctgggaatga cctggcgcgc
     2161 ggcttccttg agcacatgct tgccgccatc gggcaacttg aaacgcccag gaatgcgcgc
     2221 cgaaagctcg gccaggcgat agtcgaggaa cggtgtgcgc gcctccaggc cccaggccat
     2281 ggtcatgttg tcgacccgtt tgaccgggtc atcgaccagc atcaccgtgc tgtccaggcg
     2341 cagcgccttg tctacggcgg ccacggcgcc aggctgggcg aaatgctcac ggacgaattc
     2401 gcccgccata tcgccggtca gccaggcggg ctgcacgcag gcggcgtatt cgtcgtaatc
     2461 acggtcgaag aatgccgcgc ggtaagcgcc gaaggcatcc tcggcggcgt ccacctgcgg
     2521 ataccagtgg tagccggcga acagctcgtc ggcaccctgg ccgctctgta ccaccttgca
     2581 gtgcttggcc acctcgcgtg agagcaggta gaaggcgatg cagtcgtggc tgaccatcgg
     2641 ctcgctcatg gcgcggaaag ccgccggcag ctgttcgaga atctcgtgct cgccgatgcg
     2701 cagctgatgg tggcgagtgg cgaagtgctc ggcgatcagg tcggaatact gaaattcgtc
     2761 gccgcgctca ccgccagcat cctgaaaacc gatggagaaa gtggagaggt tttccacccc
     2821 ggcctcgcgc agcaggccga ccagcatgct cgagtcgacg ccgccggaaa gcagtacgcc
     2881 gacgtctacg gcggcgcgtt ggcggatcga taccgcttcg cgcatgccgt cgagcacgcg
     2941 ctcttgccat tcatccagac cgaaggcgat ttcctcgcgc tgcgggccga atgtcaggtt
     3001 ccaccaggtc ttctgttcac agttgccctg ggcgtcgata cgcatccagc tggcaggcgc
     3061 cagcttctcc actccagcta gcagggtgcg cggggcaggg accacggcgt ggaagtttag
     3121 atagtggttg agggcgacgg gatcgagggt cttgctgata tcaccgccgg ccagcagtgc
     3181 cggcaggctg gaagcaaaac gaaggcgttc gccggtgcgt gacaagtaca gcggcttgac
     3241 cccgaggcgg tcgcgggcga tgaacagctg ctggctgtcg cgttcccaga tggcaaaagc
     3301 gaacatgcca ttgagcttgg gcagcaggtc ggtgccccag gcgtggtagc ccttgagcag
     3361 cacttcagtg tcgccaccgg agaagaagcg atagccgagg gctttcagtt cttcgcgcag
     3421 ttccgggtag ttgtagatgg cgccgttaaa caccatcgtc aggcccagct cgctgtcgat
     3481 catcggctgg ccggaggctt cggccaggtc catgatcttc aggcggcgat ggccgagggc
     3541 tagcggaccc tggctgaaga agccgtgggc atcggggccg cgggcggcca tgtggtgggt
     3601 gattcgctcg actgcagcca ggtcggccgg tcgttgatca aaacgtaatt ctccagcgat
     3661 tgcgcacata attccttacc ggtattgccg ttggggagtg
//
LOCUS       DASRUM010000135        11432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_232879, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000135 DASRUM010000000
VERSION     DASRUM010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 11432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11432
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232879"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /gene="trmD"
                     /locus_tag="VFF22_09065"
     CDS             complement(<1..497)
                     /gene="trmD"
                     /locus_tag="VFF22_09065"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020795644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZX16995.1"
                     /translation="MPNLRVEVITLFPEMFAAISEYGITSRAVKQGLLQLTCWNPRSY
                     TTDRHHTVDDRPFGGGPGMVMKIKPLEDALLEAKQAAGGAAKVIYLSPQGRQLTQSAV
                     RELANEDALILIAGRYEGIDERFIEAHVDEEWSIGDYVLSGGELPAMVLIDAVTRLLP
                     GALGHA"
     gene            complement(503..1039)
                     /gene="rimM"
                     /locus_tag="VFF22_09070"
     CDS             complement(503..1039)
                     /gene="rimM"
                     /locus_tag="VFF22_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011059444.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HZX16996.1"
                     /translation="MSATPVSTDDLIVIGKIYSVHGVRGEVKVYSFTDPVANLLEYKS
                     WTLRREGSVKQVELVSGRGSDKFLVAKLKGLDDREEARLLAGYEICVPRNLFPELADG
                     EFYWYQLEGLKVIDQAGQLLGKIDHLLETGANDVMVVKPCTDSLDGRERLLPYTEQCV
                     LSIDLSAGEMRVDWDADF"
     gene            complement(1045..1296)
                     /gene="rpsP"
                     /locus_tag="VFF22_09075"
     CDS             complement(1045..1296)
                     /gene="rpsP"
                     /locus_tag="VFF22_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010565275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HZX16997.1"
                     /translation="MLTIRLALGGSKKRPFYKINVTDSRNPRDGSHKEEIGFFNPIAR
                     GQEVRLSVKQDRLAHWLSVGAQPSDRVAQLLKDAAKAAA"
     gene            complement(1548..2921)
                     /gene="ffh"
                     /locus_tag="VFF22_09080"
     CDS             complement(1548..2921)
                     /gene="ffh"
                     /locus_tag="VFF22_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HZX16998.1"
                     /translation="MFENLTDRLSQTLRNVTGKAKLSEDNIKDTLREVRMALLEADVA
                     LPVVKDFVNKVKDRAVGTEVSKSLTPGQAFVKIVRAELEELMGAANEDLALNAVPPAV
                     VLMAGLQGAGKTTTVGKLARFLKERKKKTVMVVSADVYRPAAIKQLETLANDIGVTFF
                     PSDLSQKPVDIAEAAIKEAKLKFIDVVIVDTAGRLAIDAEMMAEIQALHAAIKPVETL
                     FVVDAMTGQDAANTAKAFNDALPLTGVVLTKVDGDARGGAALSVRAITGKPIKFIGMG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEQAEQTLDREKAEKLTKKLKKGKGFDLEDF
                     RDQLQQMKNMGGLGGLMDKLPQMGGVNLAQMGNAQGAAEKQFKQMEAIINSMTPQERR
                     DPDVISGSRKRRIAMGSGTQVQDIGRLIKQHKQMQKMMKKFTAKGGMAKMMRGMGGMM
                     PGGMPKM"
     gene            3078..3878
                     /gene="ccsA"
                     /locus_tag="VFF22_09085"
     CDS             3078..3878
                     /gene="ccsA"
                     /locus_tag="VFF22_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZX16999.1"
                     /translation="MHPLLPSLAAACLYAGAALYQGLRLQQRSTSNKRLLVALGTLAL
                     LLHGSSLFLQMFDTSGLSLDFFKAASLIAAAVILLTLLACTRIPVENLLLLLFPLGFV
                     TVLLAQFMPSGTTQAIDEAPGILAHILLSITAYGMLTIAVFQSLLLLLQDHQLKHKHP
                     SGLIKNFPPLQTMESLLFGFLWAGWVLLSLSLLSGALFISDLFAQHLGHKTILSCFAW
                     VVFAVLLWGRHQLGWRGHKAIRWTLAGFCLLMLAYFGSKLVREFILHI"
     gene            3889..5178
                     /locus_tag="VFF22_09090"
     CDS             3889..5178
                     /locus_tag="VFF22_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HZX17000.1"
                     /translation="MDDLHSGYLLGLLIFLIMCSAFFSSSETGMLSLNRYRLKHMAKE
                     GHKGAKRVTSLLSRPDRLLGTILVGNNVVNILAASIATVLAVDIWGEAGIAIATAGLT
                     IVVLIFGEITPKTLAALRPELVAFPASRLLLLLQRLLYPVVWATSTISNVLLRLLGID
                     PSQRASDSLSTEELRSVVRESGAGMPKNRQNMLLGILDLERVTVDDIMIPRNEVTGIN
                     LDDDLESIISLLTNTTHTRLPVFRTDINQIEGIVHMRQIARLLTHNELSKEALLAACN
                     EPYFVPEGTPLSTQLINFQKQKRRIGIVVDEYGDVIGIVTLEDILEEIVGDFSNQDTL
                     RSPDIHPQEDGTQVIDGAAYIREINKALDWHLPCDGPKTLNGLVTEALESIPDSAVCL
                     KIGPYRLEILQSSENRVKSVRAWQTKPKAHTEPPARD"
     gene            complement(5144..5884)
                     /locus_tag="VFF22_09095"
     CDS             complement(5144..5884)
                     /locus_tag="VFF22_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZX17001.1"
                     /translation="MNALRAYAWWLGLALLVPIALPLALRARRNTLRLPPATGQLSGV
                     ADAELPGEPLRLLVLGESTVVGVGVDELDAALVGQLATALAVRCGRPVAWRACGENGI
                     TAAQAHERLLPQVLDQPFDLALLVFGVNDTTHLTSLPRWDAALGGMAEALAARGARVA
                     FSSVPPLQHFSALPWLLRRLLGMRAGLLDARLRQLAASLGAGHHAVELEFSAEYLARD
                     GYHPSGLGYRMWAQGLAVSLAPAARCAP"
     gene            complement(5881..7062)
                     /gene="purT"
                     /locus_tag="VFF22_09100"
     CDS             complement(5881..7062)
                     /gene="purT"
                     /locus_tag="VFF22_09100"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HZX17002.1"
                     /translation="MPRIGTPLSPSATRVLLCGSGELGKEVVIELQRLGVEVIAVDRY
                     ANAPAMQVAHRSHVINMLDGDALRAVIELEQPHYIVPEIEAIATATLVELENEGFTVI
                     PSARAAQLTMNREGIRRLAAEELGLPTSPYQFADSFEQCRAAVESIGFPCLIKPIMSS
                     SGKGQSVLKSLDDLQAAWDYAQAGGRAGKGRVIVEGFIDFDYEIALLTVRHSGGTTFC
                     APVGHRQVKGDYQESWQPQPMSAKAQAEAERIALAVTGSLGGRGLFGVELFVKGDQVW
                     FCEISPRPHDTGLVTLISQDLSEFALHARAILGLPIPAIRQFGPSASAVILVEGESQQ
                     VSFGNLAAALAKPDTALRLFGKPEVSGQRRMGVALARDESLQAARAKATRAAQAVKVE
                     L"
     gene            complement(7090..7281)
                     /locus_tag="VFF22_09105"
     CDS             complement(7090..7281)
                     /locus_tag="VFF22_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HZX17003.1"
                     /translation="MQPQERPVASPCVHVCALDDEDICIGCQRSVAEITRWSLMDNAE
                     RREVLVQCHERAKAKGILL"
     gene            complement(7281..7805)
                     /locus_tag="VFF22_09110"
     CDS             complement(7281..7805)
                     /locus_tag="VFF22_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003209603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HZX17004.1"
                     /translation="MKYRLGSSQVEAHADSWVAPNATLVGKVKLEPGASVWFNAVLRG
                     DNELIHIGENSNVQDGTVMHTDMGFPLSIGTGVTIGHNAMLHGCSVGDYSLIGINAVI
                     LNGAKIGKYCIIGANSLIGEGKEIPDGSLVMGSPGKVVRELTEPQKKMLEASAAHYVH
                     NAQRYARDLQEQDD"
     gene            complement(7824..8429)
                     /locus_tag="VFF22_09115"
     CDS             complement(7824..8429)
                     /locus_tag="VFF22_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HZX17005.1"
                     /translation="MLDELLLRMRGYSPHILPTDKRYPEAAVLVPITRSDEPEVVLTL
                     RASGLSTHGGEVAFPGGRRDPEDADLVETALREAEEEIGLPPGLVEVIGPLSTLVSRH
                     GIQVTPYVGVVPDFVEYKPNDGEIAAVFAVPLAFFRDDPRETTHRIDYLGRSWYVPSY
                     RYGEYKIWGLTAIMLVELVNLAFDDVHIDMHQPPEHFIKLT"
     gene            8592..9149
                     /locus_tag="VFF22_09120"
     CDS             8592..9149
                     /locus_tag="VFF22_09120"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZX17006.1"
                     /translation="MKFCSNCGGAINRIIPQGDNRLRHVCAQCLTVHYQNPRIIAGCL
                     PVWGEQVLLCRRAIEPRRGYWTLPAGFMENGETLEQAAARETHEEACARVRSLSLYAL
                     FDLPHINQVYMLFRAELVDLDFAPGEESLEVQLFHEQDIPWSELAFPTIGRTLECYFA
                     DRREQHYPVRNEPLEALRAHYKKAF"
     gene            9165..9668
                     /locus_tag="VFF22_09125"
     CDS             9165..9668
                     /locus_tag="VFF22_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZX17007.1"
                     /translation="MRWLFALLCLTVALTGHASTTPSLNGKTIDKVLVVKSERKLHLI
                     SRGNTLKSYRISLGKQPKGAKQREGDLRTPEGFYWIDWRKTSDKYNLSMHISYPNARD
                     QAQARKSGVSAGGMIMVHGTPLDEEYPEWMFHTLDWTEGCIAMRNIDMREVWAVVKDG
                     TLIEIRP"
     gene            complement(9714..10031)
                     /locus_tag="VFF22_09130"
     CDS             complement(9714..10031)
                     /locus_tag="VFF22_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqfO family protein"
                     /protein_id="HZX17008.1"
                     /translation="MYKLCFYVPETHLDAVKSAVFSAGGGRIGQYDQCCWQTLGQGQF
                     RALEGSQPFLGQPGQVEQVAEWKVEMAVADELIHDAVKAMKKQHPYETPAFDVWRLSD
                     IQF"
     gene            complement(10190..10459)
                     /locus_tag="VFF22_09135"
     CDS             complement(10190..10459)
                     /locus_tag="VFF22_09135"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HZX17009.1"
                     /translation="MSSKPAPILPEVRQVKPGDTLLLCRCGRSSQLPDCISACPDALE
                     LQPEREQFLLLCRCACSQRLPYCDGSHNQPVSGLKARWRRFWRGL"
     gene            complement(10478..>11432)
                     /locus_tag="VFF22_09140"
     CDS             complement(10478..>11432)
                     /locus_tag="VFF22_09140"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HZX17010.1"
                     /translation="RLLQRQWAQTSTQIQRLRDNVQCAEQEFDTILDEDNPGLSAKLS
                     FDVNQDISAPYIRKGVRPQIAVLREQGVNGQVEMAAAFDRAGFNAIDVHMSDILSGRV
                     SLDEFKGLVACGGFSYGDVLGAGEGWAKSILFNSRARDGFQAFFERKDSFSLGVCNGC
                     QMMSNLHELIPGTEFWPHFVRNRSEQFEARVAMVQVQESASIFLRGMAGSRMPIAIAH
                     GEGHAEFENEEALLEADLSGCVAMRFIDNHGKVTETYPANPNGSPRGITGLTSRDGRV
                     TIMMPHPERVFRTVQNSWKPDDWQEDAGWLRMFRNARVWVD"
BASE COUNT         2388 a         3674 c         3154 g         2216 t
ORIGIN      
        1 gcatgaccca atgcaccagg caaaaggcgc gttaccgcgt cgatcagcac catggccggc
       61 agctcaccgc cagacaggac gtagtcccca atcgaccatt cttcatcgac atgcgcttca
      121 ataaagcgct catcaattcc ttcgtagcgg ccggcaatca ggattaacgc atcctcattg
      181 gccagttcgc gtactgctga ctgcgtcagc tggcgacctt gcggcgacag gtaaatcacc
      241 ttggccgcac cacctgcagc ttgcttcgcc tcaagcaaag catcttcaag cggcttgatc
      301 ttcatcacca tgcctgggcc accgccaaaa ggccgatcat ccacggtgtg gtgacggtct
      361 gtggtgtagc tacgcgggtt ccagcaggtc aattgcaaca aaccctgctt caccgctcgg
      421 ctggtaatgc cgtattcgct gatagcggca aacatttccg ggaacagcgt aatgacttca
      481 acgcgcagat ttggcatcag gcttagaagt ccgcatccca gtcgacccgc atttcgccag
      541 cacttaggtc aatcgacagc acgcattgct cggtataggg caacaggcgc tcacgaccat
      601 ccaggctgtc ggtgcagggt ttgaccacca tcacatcgtt ggcaccggtt tctaacaagt
      661 gatcaatctt gccgagcaat tgccctgcct gatcaataac cttaagacct tccaactggt
      721 accagtagaa ctcaccgtcg gctaattcag ggaacaggtt gcgcggcaca cagatctcat
      781 aaccagcgag aaggcgagcc tcttcacgat catcaagacc cttgagcttc gcgaccagga
      841 acttatcact cccgcgtccg ctgaccagct ctacctgttt gacactgcct tcgcgcctaa
      901 gcgtccagga cttgtactcc aacaggtttg caacaggatc agtaaaggaa tacaccttca
      961 cttcaccgcg aacgccatgt acagagtaga tcttgccgat aacgatcaaa tcatcggtag
     1021 aaacaggcgt cgcactcata ttgcttaggc cgcagcctta gcagcgtcct tcaacaactg
     1081 agcaacacga tcagaaggct gcgcgccaac gctcagccaa tgggccaggc ggtcttgctt
     1141 cacggacagg cgaacttcct gaccacgagc gatcgggttg aagaaaccga tttcttcttt
     1201 atgcgaaccg tcgcgtgggt tacggctgtc ggtcacgttg attttgtaga acgggcgctt
     1261 tttggagccg ccaagggcaa gacgaatcgt tagcatgtga acatagttcc tgtagtcggt
     1321 gctgcaaatc taaatgcacg gcgggcacgg gtgcccgaaa ggccgcatat tctaaggaat
     1381 atccggattt ttgcaaatgg ctttttcagg cgcataggac tgtgcaatca agatttgcca
     1441 gagggccgct gtgcaaaccg gccaatcagc tcccgcaaaa ggcgggttta ctgtaaatac
     1501 ccgccttcag cggggtctgc gccgacatcg ctgccgacgc ggattcctta catcttcggc
     1561 atgccgcccg gcatcatccc gcccatgcca cgcatcatct tggccatacc gcctttagcg
     1621 gtgaatttct tcatcatctt ttgcatttgc ttgtgctgct tgatcaggcg gccaatgtcc
     1681 tgcacctggg tgccggaacc catggcgatg cggcgcttgc gcgagccact gatcacatca
     1741 ggatcacggc gctcctgagg cgtcatcgag ttgatgatcg cttccatctg cttgaactgt
     1801 ttctcggcag cgccctgggc attgcccatc tgcgccagat tgacgccgcc catctgtggc
     1861 agcttgtcca tcaggccccc aaggccgccc atgttcttca tctgctgcag ctgatcgcgg
     1921 aagtcttcga ggtcgaagcc cttgcccttc ttcagcttct tggtgagctt ctcggccttt
     1981 tcgcgatcca gagtctgctc ggcttgctcg atcaggctga gcacgtcgcc catgccgaga
     2041 atacgcgagg caatccggtc aggatggaag ggctcgagcg cctcggtctt ctcgcccata
     2101 ccgataaact tgatcggttt gccggtaatc gcacgcaccg acaacgccgc accgccgcgc
     2161 gcatcaccgt cgactttggt cagcaccacg ccagtcagcg gcagcgcatc attgaaggct
     2221 ttggcggtat tggcggcatc ctggccggtc atggcgtcga ccacgaacag ggtttccacc
     2281 ggtttgatcg ccgcatgcag cgcctggatc tccgccatca tctcggcatc gatggccaga
     2341 cgaccggcgg tatcgacgat caccacatca ataaacttga gcttggcttc cttgatcgcc
     2401 gcttcagcga tatccaccgg cttctggctg agatcagagg ggaagaaggt cacgccaata
     2461 tcattggcca gggtttccag ctgcttgatc gccgccggac ggtaaacgtc ggccgacacc
     2521 accatcacgg tctttttctt gcgctcttta aggaagcgcg ccagcttgcc aacggtggtg
     2581 gtcttacccg caccctgcag acccgccatc agcaccacgg ctggcggcac ggcattcagc
     2641 gccaggtctt cgttggccgc gcccatcagc tcttcgagct cggcacggac gatcttcaca
     2701 aaagcctggc ccggcgtcag gctcttcgac acctcggtgc cgaccgcacg atccttgacc
     2761 ttgttgacga agtccttgac caccggcaag gcaacgtcag cctcgagcaa ggccatgcgc
     2821 acttcacgca aggtgtcctt gatgttgtct tcgctcagct tggctttgcc ggtgacattt
     2881 cgcagggtct gcgagaggcg gtcagtcaga ttttcgaaca tgcgcgttcc tttcaggctc
     2941 taccgagccg agggtgggct tgcaacaggt tgcggattat agcgaagact gcaaggcacc
     3001 gacaccgcca gacgctttcg tggcacacgg gttgtatgcc acactgaccg cctttcgggt
     3061 ttgcctgaca aggatttatg caccctctgc tacccagcct cgccgctgcc tgcctctatg
     3121 ccggagccgc gctctatcaa ggcctgcgcc tgcaacagcg cagcacatcc aacaagcgcc
     3181 tgctggttgc actcggcaca ctggcgttgc tactgcacgg cagcagcctg tttttgcaga
     3241 tgttcgatac cagcggtctg tcgctggact tcttcaaggc cgccagcctg attgccgccg
     3301 ccgttatcct gctgacgcta ctggcctgca cgcgaatccc ggtggaaaac ctgctgttgc
     3361 tgctattccc cctgggcttt gtcacggtgc tgctcgccca atttatgccc agcggcacga
     3421 cccaggccat tgatgaagcg cctggcatcc tggcgcatat cctgctgtcg atcaccgcct
     3481 acggcatgct gaccattgcg gtattccagt cactgctgct gctattgcag gatcaccaac
     3541 tcaagcacaa gcacccgtcc gggctgatca agaacttccc acccctacag accatggaaa
     3601 gcctgctgtt cggctttctc tgggccggct gggtattgct gtcactctct ctgctgtctg
     3661 gcgcgctgtt tatcagcgac ctgtttgccc agcacctggg gcataaaacc atcctctcgt
     3721 gctttgcctg ggtggtgttt gccgtgctgt tgtggggccg ccatcaactc ggctggcgcg
     3781 gccacaaagc cattcgctgg accctggcgg gcttctgcct gctgatgctg gcctacttcg
     3841 gcagcaagct ggttcgcgaa tttatcctgc atatctgaga ccccttccgt ggacgacctt
     3901 cactccggct acctgcttgg gctgctgatt tttctgatta tgtgctcggc attcttctcc
     3961 agctcggaga ccggcatgct cagccttaac cgctatcgcc tgaaacacat ggccaaagaa
     4021 gggcataagg gcgccaagcg cgtcaccagc ctgcttagcc gcccggatcg cctgctcggc
     4081 accatcctgg ttggtaacaa cgtggtcaac atcctcgctg cctccatcgc tacggtgctg
     4141 gcagtggata tctgggggga agccgggatc gcgattgcca ccgcaggctt gaccatcgtg
     4201 gtgctgattt tcggtgaaat caccccaaaa accctcgccg cgctgcgccc agaactggtg
     4261 gcttttcccg ccagccgctt gctgctgttg ctgcaacgcc tgctttatcc agtggtctgg
     4321 gccaccagca ccatcagcaa cgttctgctg cgcctgctgg gtatcgaccc gtcgcaacgc
     4381 gccagcgaca gtttgtctac cgaagagctg cgcagcgtgg tgcgcgagtc cggtgccggc
     4441 atgccgaaga atcgccagaa catgctgctc ggcatcctgg atcttgagcg ggtgacggtg
     4501 gatgacatca tgattccgcg caacgaagtc accggcatca acctcgatga cgaccttgaa
     4561 agcatcatca gcctactgac caacaccacc catacgcgtc tgccggtatt tcggaccgac
     4621 atcaaccaga tcgaaggcat cgtgcatatg cgccagattg cccggctgct gacgcacaac
     4681 gagctgagca aagaagcgct attggcggcc tgcaacgaac cctacttcgt acccgaaggc
     4741 acgccgctgt cgacccagct gatcaatttt cagaagcaga aacgccggat cggtatcgtt
     4801 gtcgatgaat acggcgacgt gattggcatc gtcaccctgg aagatatcct cgaagaaatc
     4861 gtcggcgact tcagcaacca ggacaccctg cgcagcccgg acattcaccc tcaggaagac
     4921 ggcacccagg taattgatgg cgccgcctat atccgcgaaa tcaacaaggc actggactgg
     4981 cacctgccct gcgatggccc gaaaaccctc aacggcctgg tcaccgaggc gctagaaagc
     5041 atccccgaca gcgccgtgtg cctgaagatc ggcccatatc gcctggaaat tctgcagtcg
     5101 tcagagaacc gggtaaaaag cgtacgcgcc tggcaaacca agcctaaggc gcacaccgag
     5161 ccgccggcgc gagactgacg gccagcccct gcgcccacat gcgatagccc agacctgacg
     5221 ggtgataacc atcacgcgcc aggtactcgg cggaaaactc cagttcaacc gcgtgatgcc
     5281 ccgcccccag gcttgccgct agctggcgca aacgcgcatc cagcaggccg gcgcgcatac
     5341 cgagcaaacg ccgcagcaac cagggcaagg cactgaaatg ctgcagcggc ggcacactgc
     5401 taaacgccac ccgagcgccg cgcgcggcca acgcttcagc catgccgccg agcgcggcat
     5461 cccaacgtgg taatgaggtc aggtgggtgg tgtcattgac gccgaacacc aataacgcca
     5521 gatcaaaggg ctgatccagc acctgcggca acagccgctc atgggcctgt gcagccgtaa
     5581 tgccgttctc gccgcaagcc cgccaggcaa ccggacggcc gcaacgtaca gccagcgccg
     5641 tagccagctg cccgaccagc gcggcgtcca gctcatccac ccccacacca acgactgtcg
     5701 actcacccaa caccagcaag cgcaatggct cgcccggtaa ttccgcgtca gccacaccgc
     5761 tcaactgccc tgtggctggc ggtaaacgca gggtgttacg ccgcgcgcgc agtgccagcg
     5821 gcaaggcaat cggcaccaac agcgccaacc ctagccacca ggcataagcg cgcagcgcat
     5881 tcacagctcg accttgaccg cctgcgccgc acgcgtggcc ttggcccgcg ccgcttgcaa
     5941 tgactcgtca cgagccagcg ccacacccat acgtcgctga ccgctgactt ccggcttgcc
     6001 gaacaggcgc aaagcggtat ccggtttagc cagagccgcc gccagattgc cgaagctgac
     6061 ctgctgcgac tcgccctcga ccaatatcac cgcagaagcc gacgggccga actgacgaat
     6121 cgccggaatc ggcaaaccga gaattgcccg cgcatgcagg gcgaattcgg acaggtcctg
     6181 ggaaatcagg gtgaccaaac cagtgtcgtg cggacgcggc gatatttcgc agaaccacac
     6241 ctggtcgcct ttgacgaaca gctcgacgcc aaacagcccg cgcccaccca gggagccggt
     6301 aactgccagg gcgatgcgct cggcttcggc ctgagccttg gcgctcatcg gctgcggctg
     6361 ccaggactcc tgatagtcgc ccttgacctg acgatggccc acaggcgcgc agaaggtggt
     6421 gccgccactg tggcgcacgg taagcagcgc gatttcataa tcaaaatcga taaagccctc
     6481 gacgattact cggcccttgc cggcgcggcc accggcctgg gcgtaatccc aggcggcctg
     6541 cagatcatcc aggcttttca gcactgattg gcccttgccg gaagagctca tgatcggttt
     6601 gataaggcag ggaaagccaa tgctctcgac tgcggcgcgg cactgctcga aggagtcggc
     6661 aaactgataa ggcgaggtcg gcaggcccag ctcttcggcg gccaggcggc gaatgccttc
     6721 acgattcatg gtcagctgcg cagcgcgggc gctggggatt acggtaaagc cttcattctc
     6781 cagctcgacc agggtcgcag tggcaatcgc ctcgatttcc gggacgatgt aatgcggttg
     6841 ctccagctcg atcaccgccc gcagcgcgtc gccgtcgagc atattaatca catggctgcg
     6901 gtgcgccacc tgcatggccg gggcattggc gtagcgatcc acggcgatta cctcgacgcc
     6961 taggcgctgc agctcgatca ccacttcctt gcccagctcg ccagagccgc acaacagcac
     7021 acgggtcgcg ctcggcgaca aaggggttcc gatacggggc atggtgaatc ctcaagctga
     7081 tcaagaaagt tacagcagaa tgcctttagc cttcgcccgc tcatgacact gaactaacac
     7141 ctcacggcgc tcggcgttat ccatcaggct ccagcgggtg atttcggcca ccgagcgctg
     7201 gcagccgata cagatatctt catcgtccag cgcgcagaca tgcacgcagg gcgaggcgac
     7261 cggacgttcc tgcggctgca tcaatcgtcc tgctcctgca gatcgcgggc atagcgctga
     7321 gcgttgtgca cgtaatgggc ggcgctggct tcaagcatct tcttctgcgg ctcggtcagt
     7381 tcgcgcacca ccttaccggg cgaacccata accagcgagc catcgggaat ttccttgcct
     7441 tcaccgatca ggctgttggc gccaatgatg cagtacttgc cgattttcgc gccattgaga
     7501 atcaccgcat tgatgccgat caggctgtaa tcccccaccg agcagccgtg cagcatggcg
     7561 ttgtggccga tggtcacgcc agtgccgatg gacagtggaa agcccatatc ggtgtgcatc
     7621 acggtgccgt cctgcacatt gctgttctcg ccgatatgaa tcagctcgtt gtcgccgcgc
     7681 agcacagcgt taaaccacac gctggccccc ggttccagct tgaccttgcc taccagagtg
     7741 gcattcggtg caacccagct gtcggcatgg gcctcgactt gggacgaacc caggcggtat
     7801 ttcatgggtg tttcctcata ggctcaggtg agcttgataa agtgctcggg cggctggtgc
     7861 atatcgatat gcacatcgtc aaacgccagg ttgaccaact caaccagcat gatcgccgtc
     7921 agcccccaga ttttatattc gccataacgg taactcggca cataccagct gcgcccgagg
     7981 tagtcgatac ggtgggtggt ttcccgcggg tcgtcgcgaa agaacgccag cggcacagca
     8041 aatacggcag caatctcacc gtcgttgggt ttgtactcga caaaatccgg taccacaccg
     8101 acataaggcg tgacctgaat gccgtgacgc gacaccaggg tgctaagcgg cccgataacc
     8161 tcgaccaggc cgggcggcaa gcctatttcc tcttccgcct cacgcagggc ggtttcgacc
     8221 aaatcggcat cttccggatc acgccgacca ccgggaaacg ccacctcacc accgtgggtg
     8281 gacaaaccgc tggcgcgcaa agtcagtacc acttccggct cgtcgctgcg ggtgatgggc
     8341 accagcactg cggcctcagg gtagcgcttg tcggtcggca gaatgtgcgg gctatagcca
     8401 cgcatgcgaa ggagcaactc gtccagcatg gggattctca gtcttttatt ctgcggtcga
     8461 tcatggcacg aaagcagcgc acggcccaag ccccgcgcca tagcaggtaa ttattcaggc
     8521 gctcgataaa ccccgcttgc cgagcacaca acacgcgcgc caagatagcc aacacgcagc
     8581 gagggtctgg catgaagttc tgtagcaact gtggcggcgc gatcaaccgc atcatcccgc
     8641 agggcgacaa ccgcctgcgc cacgtctgcg cgcagtgcct gaccgtgcac tatcagaatc
     8701 cgcgaatcat cgccggctgt ctgccagtgt ggggcgaaca ggtgctgctg tgccgccgcg
     8761 ccatcgaacc acggcgcggt tactggacct tgccggccgg ctttatggag aacggcgaaa
     8821 ccctggaaca ggccgccgcc cgcgaaaccc atgaagaagc ctgcgcgcgc gtgcgcagcc
     8881 tcagcctcta tgccctgttc gacctgccac atatcaatca ggtgtatatg ctgtttcgcg
     8941 ccgagctagt cgacctggac tttgcccccg gtgaagaaag cctggaggtt cagctatttc
     9001 acgaacagga catcccctgg tcagagctgg ctttcccgac catcgggcgt accttagaat
     9061 gctacttcgc cgaccggcgc gagcagcact acccggtccg caacgagccg cttgaagcct
     9121 tgcgcgcaca ttacaagaaa gccttttgat ccatgggaat tatgatgcgc tggttgttcg
     9181 ccctactctg cctgaccgtt gccctgactg gccacgccag caccacccca agcctcaacg
     9241 gcaagaccat cgacaaagtg ctggtggtca aatccgaacg caagctgcac ctgattagcc
     9301 gcggcaacac cctcaagtcc taccgcatct ccttgggtaa acagcctaaa ggagctaagc
     9361 agcgcgaagg cgacctgcgc accccggaag gcttctattg gatcgactgg cgcaagacca
     9421 gcgacaagta caacctgtcc atgcacatct cctaccccaa cgcccgcgac caggcgcaag
     9481 cgcgcaagag cggtgtatcg gcgggcggca tgatcatggt tcacggcact ccgctggacg
     9541 aggaataccc tgagtggatg ttccacacgc tggactggac cgaaggctgc attgccatgc
     9601 gcaacatcga tatgcgcgag gtctgggcag tagtcaaaga cggcacactg atcgaaatca
     9661 gaccctgatc agcagacata aaaaaggcgc cttcagggcg ccttttttat gagttagaac
     9721 tggatgtccg acagccgcca cacatcaaaa gccggcgtct cgtagggatg ctgcttcttc
     9781 atggccttga ccgcgtcatg gatcagctca tcggccacgg ccatctcgac tttccactct
     9841 gccacctgct caacctgccc aggctggccg agaaatggct ggctgccctc cagagcacgg
     9901 aactggccct gccccagggt ttgccaacaa cactgatcgt actgaccaat acgtccgcca
     9961 ccggcgctga ataccgcgct tttcacggca tccagatgag tttcaggcac ataaaagcac
    10021 aacttgtaca tcgaagtctc cgccggcaag actgataaaa acagttcgat aatgatgcca
    10081 cacaataatg gcactttgcc ttatcgggta ttcgcgcagc ccatcacact tgcaacttat
    10141 gaccggaaaa ttcgcggtgt gttcaaccta ttagcgcaat cagctacgcc tacagaccgc
    10201 gccagaagcg ccgccagcgg gcctttaagc cggacaccgg ctggttatgg ctaccatcac
    10261 agtaaggtag acgttgcgag caagcgcaac ggcaaagcag cagaaactgc tcacgctcag
    10321 gctgcagttc aagcgcatca gggcaggcag aaatacaatc gggaagctgg gacgaacgtc
    10381 cgcagcggca cagcagcaag gtatcgccag gtttgacctg gcgcacctca ggcagaatcg
    10441 gcgcaggctt actcgacata agcctgcgcc aaccggatca atccacccac acgcgagcat
    10501 tgcggaacat ccgcagccag ccggcatctt cctgccagtc atctggtttc caggagttct
    10561 gcacggtgcg gaatacccgc tccgggtgcg gcatcatgat ggtcacgcgg ccgtcgcggc
    10621 tggtcagacc ggtgatcccg cgcggcgaac cattagggtt ggccggatag gtttcggtga
    10681 ccttaccgtg gttgtcgata aagcgcatag ccacgcagcc ggacagatcg gcctcgagca
    10741 gggcttcctc gttctcgaac tcggcatgcc cttcaccgtg agcgatggcg attggcatac
    10801 gcgaaccggc catgccacgc aggaagatcg acgccgactc ctgcacctgg accatggcca
    10861 cgcgcgcctc gaactgctcg gaacggttac gcacgaagtg cggccagaac tcggtgccgg
    10921 ggatcagctc gtgcaggttg ctcatcatct ggcagccgtt gcacacgcca agactgaagc
    10981 tgtcctttcg ttcgaagaac gcctggaagc catcacgggc gcggctgttg aacaggatcg
    11041 acttggccca gccttcgcca gcaccgagca cgtcgccgta ggaaaagccg ccgcaggcga
    11101 ccaagccctt gaactcatcc aggctgacgc ggccgctaag gatatcgctc atgtgtacgt
    11161 cgatcgcatt gaaaccggcg cggtcgaagg ctgcagccat ttccacctgg ccgttgacgc
    11221 cctgctcgcg cagtacagcg atctgcggac gcacaccctt gcggatatac ggcgcgctga
    11281 tgtcctgatt gacgtcgaag ctgagcttgg ccgacaggcc cggattgtcc tcatcgagga
    11341 tggtgtcgaa ttcctgctcg gcgcactgca cgttgtcgcg cagacgctga atctgggtgc
    11401 tggtttgcgc ccactggcgc tgcagcaggc gg
//
LOCUS       DASRUM010000136         5456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2235095, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000136 DASRUM010000000
VERSION     DASRUM010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2235095"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VFF22_09145"
     CDS             complement(<1..828)
                     /locus_tag="VFF22_09145"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HZX17011.1"
                     /translation="MALIVQKFGGTSVGTVERIQQVAAKVKKFRENGDDIVVVVSAMS
                     GETNRLIDLAKQVSDGQPVPRELDVMVSTGEQVTIALLAMALIKLGVPAVSYTGNQVR
                     ILTDSAHNKARILQIDDQKLRADLKAGRVVVVAGFQGVDEHGNITTLGRGGSDTTGVA
                     LAAALKADECQIYTDVDGVYTTDPRVVPQAQRLEKITFEEMLEMASLGSKVLQIRSVE
                     FAGKYNVPLRVLHSFQEGPGTLITLDEEESMEQPIISGIAFNRDEAKLTIRGVPDIPG
                     "
     gene            complement(943..3567)
                     /gene="alaS"
                     /locus_tag="VFF22_09150"
     CDS             complement(943..3567)
                     /gene="alaS"
                     /locus_tag="VFF22_09150"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HZX17012.1"
                     /translation="MKSAEIREAFLSFFEQKGHTRVASSSLIPGNDPTLLFTNAGMNQ
                     FKDCFLGLEKRAYTRAATSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     RDAIHFAWEFLTGENWLNLPKDKLWVTVYATDDEAFDIWNKEIGVPAERMIRIGDNKG
                     APYASDNFWTMGDTGPCGPCTEIFFDHGEHIWGGPPGSPEEDGDRYIEIWNNVFMQFN
                     RTADGVLHPLPAPSVDTGMGLERVSAVLQHVNSNYEIDLFQSLLNAAAKAIGCANEGQ
                     ASLKVVADHIRSCGFLIADGVTPSNEGRGYVLRRIIRRACRHGNKLGAKGSFFYQIVA
                     ALVAEMGDAFPELKQQQAHIERVLKTEEEQFAKTLEQGLKILEQDLAELKGTVIPGDV
                     IFKLYDTFGFPMDLTGDIARERELTLDEEGFEREMQAQRERARSASAFGMDYNSLVKV
                     DSDTAFLGYQGTSGSGKIIALFKAGQAVDSLSEGEEGVVVLDQTPFYAESGGQVGDCG
                     FLEGAGVRFDVRDTTKAGGAFLHHGVAVKGGLSFGTSVKAEVDASVRQATALNHSATH
                     LLHAALRQVLGEHVTQKGSQVDSQRLRFDFSHFEAITPAQLKELETIVNREVRGNSEV
                     ETLETDIETAKAKGAMALFGEKYGDQVRVLSMGGDFSVELCGGTHVKRTGDIGLFKIT
                     SESGVAAGVRRIEAVTGAAAFAYLNGAEEQLKEAAALVKGSRDNLLDKLAGVLERNRQ
                     LEKELEQLKAKAASAAGNDLAGSAVDVKGIKVLTARLDGLDGKALLAMVDQLKNKLGS
                     AVILLGGVQDDKVVLVAGVTQDLTAKLKAGELMKQAAAAVGGKGGGRPDMAQGGGVDA
                     AALDGALALAVTFAEQGL"
     gene            complement(3690..4694)
                     /gene="ltaE"
                     /locus_tag="VFF22_09155"
     CDS             complement(3690..4694)
                     /gene="ltaE"
                     /locus_tag="VFF22_09155"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003204778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HZX17013.1"
                     /translation="MPVIDLRSDTVTQPTPGMREAMQAAELGDDVYGEDPTVNRLEQY
                     LAAELGFEAALFVPTGTMSNLLGLMAHCARGDEYIVGQQAHTYKYEGGGAAVLGSIQP
                     QPIDGEADGSLDLAKVEAAIKQDDFHFARTRLLALENTMQGKVLPQTYLQAARELTTR
                     RGLALHLDGARLYNAAVKLGVPAREITQYFDSVSVCLSKGLGAPIGSVLCGSVELIGR
                     ARRLRKMVGGGMRQAGMLAAAGLYALQHQVARLAEDHANAERLAAGLRELGYTVEPVQ
                     TNMVYGQVGEQADALKAFCAERGIKLSAAPRLRMVTHLDISTADIDQVLAAFAEFRSH
                     "
     gene            complement(4871..>5456)
                     /locus_tag="VFF22_09160"
     CDS             complement(4871..>5456)
                     /locus_tag="VFF22_09160"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HZX17014.1"
                     /translation="RTRLHYDLHTAIRASKIEQFALYPFQEGRPRSQRELARLSAAGI
                     EAVLLHNKSSITFSAYTYTQLGAEAFTLELGKARPFGQNELVNLDLLELHLQALIEGR
                     EPEGATDDLGELKLFAVAREVIKHSDAFKLHLPADIENFSELPIGYLLAEDLADTRWV
                     VDEPGARIIFPNPKVKNGLRAGILIVPAEGVQLA"
BASE COUNT         1040 a         1726 c         1606 g         1084 t
ORIGIN      
        1 gcccgggatg tccggtacgc cacggatggt cagcttggct tcgtcgcggt tgaaagcgat
       61 gccggagatg atcggctgtt ccatggattc ctcttcatca agggtaatga gggtgcccgg
      121 cccctcttgg aagctgtgca gcacgcgcag cgggacgttg tacttgccgg cgaactccac
      181 cgaacgaatc tgcagcacct tggacccgag gctggccatt tccagcatct cttcgaaggt
      241 gattttttcc agacgctgag cctgcggcac cacacgtggg tcggtggtgt aaacaccatc
      301 aacgtcggtg tagatctggc actcatcggc cttcagcgca gcagccaacg ccaccccggt
      361 ggtgtcggaa ccgccacgac cgagggtggt gatattgccg tgctcgtcga caccctggaa
      421 gccggcgacc accaccacac gaccggcctt gaggtcggca cgcagcttct ggtcatcgat
      481 ctgcaggatg cgcgctttgt tgtgcgcgct gtccgtcagg atgcgcacct ggttgccggt
      541 ataggacacc gccggcacgc ccagcttgat cagcgccata gccagcaggg caatcgtcac
      601 ctgctcaccg gtggaaacca tcacgtcgag ttcacgtggt accggctgac catcactgac
      661 ctgtttggcc agatcgatca ggcgattggt ttcaccgctc atggcggaaa ccacaaccac
      721 aatgtcatcg ccgttttcgc ggaatttctt caccttcgcg gccacctgct gaatacgctc
      781 aacagtgccg acggaggtgc cgccaaattt ctgtacgatc aaagccattt caaagccgcc
      841 tcagcccgtg aagggcgccc attaaacata caactcaata ccctgccaag gcccgccggg
      901 cgtataccgg gccttgggct gtccatccgg ggaatcaggc gtttacaaac cctgctcggc
      961 aaaagtcacc gccaacgcca gcgcaccatc aagggccgcc gcatcaacgc caccgccctg
     1021 agccatgtcc gggcgaccgc cgcccttacc gccgacggcc gcagccgcct gcttcatcag
     1081 ctcgccggct ttcaatttgg cggtcagatc ctgggttaca ccggccacca gcacgacttt
     1141 atcgtcctgc acgccaccga gcaggatgac tgcgctgcca agcttgttct tcaattggtc
     1201 gaccatcgcc agcagcgcct tgccgtccag accgtcgaga cgcgcagtca gcaccttgat
     1261 gcctttgacg tcgacagcgg aaccggccag gtcgttgccc gccgcgctgg cggccttggc
     1321 cttgagctgc tccagctctt tttccagctg acggttgcgc tccagcacac cggccagctt
     1381 gtccagaagg ttgtcgcgac tgcctttgac cagcgccgcg gcttctttca gctgctcttc
     1441 cgcgccattc aggtaggcaa acgctgccgc gccggtaacc gcttcaatcc gacgcacgcc
     1501 agcggccaca ccgctttcgc tggtgatctt gaacaggccg atatcgccag tgcgcttgac
     1561 gtgagtgccg ccgcacagct caacggagaa gtcaccgccc atactcagca cgcgcacctg
     1621 atcgccgtat ttttcaccga acagcgccat ggcacctttg gccttggcgg tttcgatatc
     1681 ggtttccagg gtttccactt cgctgttgcc gcgcacctca cggttgacga tggtttccag
     1741 ctctttcagc tgcgctggag tgatcgcctc gaagtggctg aagtcaaaac gcagacgctg
     1801 actgtcgacc tgcgagccct tctgggtcac gtgctcaccc agcacctgac gcagcgcagc
     1861 atgcagcaag tgagtggccg agtggttgag cgcagtggcc tgacgcacgc tggcgtccac
     1921 ttcggccttg accgaggtgc cgaagctcaa gccgcctttg acggcaacgc cgtggtgcag
     1981 gaatgcgccg ccagccttgg tggtatcacg cacgtcaaaa cgcacgcctg cgccttcaag
     2041 gaagccacag tcaccgacct ggccaccgga ttcggcgtag aacggcgttt ggtcaaggac
     2101 tactacgccc tcctcgcctt cactcaggct atccacggcc tgaccggcct tgaacagcgc
     2161 aatgatcttg ccgctgccac tggtgccctg atagccgagg aacgcggtgt cgctgtcgac
     2221 cttgaccagg ctgttgtagt ccatgccaaa ggcgctggca gaacgggcgc gctcgcgctg
     2281 cgcctgcatt tcgcgctcaa agccctcttc atccagggtc aattcgcgct cacgggcgat
     2341 gtcaccagtg aggtccatcg ggaagccgaa ggtgtcgtac agcttgaaga tcacatcgcc
     2401 cggaatcacc gtgcctttta gctcagccag atcctgctcg agaatcttca ggccctgctc
     2461 cagggtcttg gcaaactgct cttcttcggt tttcagcacg cgctcgatat gcgcctgctg
     2521 ctgtttcagc tcggggaagg catcgcccat ctcggccacc aatgcggcaa cgatctggta
     2581 gaagaagctg cccttggcgc ccagcttgtt accgtggcgg caggcgcgac ggatgatgcg
     2641 gcgcagcacg tagccacgac cttcgttgga cggtgtcacg ccgtcggcga tcaggaagcc
     2701 gcaggaacgg atatggtcag ccaccacttt cagcgaagcc tggccctcat tggcacaacc
     2761 aatggccttg gccgccgcat tcagcaggct ctgaaacagg tcgatctcgt agttcgaatt
     2821 gacgtgctgc agcaccgcac taacgcgctc caggcccatg ccggtgtcca cgctcggcgc
     2881 cggcagcggg tgcaatacgc cgtctgcggt gcggttgaac tgcataaaga cgttgttcca
     2941 gatttcgatg tagcgatcac cgtcttcttc tggcgagccg ggcgggccac cccagatatg
     3001 ctcgccgtgg tcgaagaaaa tctcggtgca cgggccgcac gggccggtgt cgcccatggt
     3061 ccagaagttg tcggaagcgt acggcgcgcc tttgttgtcg ccgatacgga tcatgcgctc
     3121 ggccgggacg ccgatttcct tgttccagat atcaaaggct tcatcgtcag ttgcgtagac
     3181 ggtgacccag agcttgtcct tgggcaggtt cagccagttt tcgccggtga ggaattccca
     3241 ggcgaagtga atggcgtcgc gcttgaaata gtcgccaaag ctgaagttac cgagcatttc
     3301 gaagaaggtg tggtgacgtg cggtgtaacc gacgttttcc aggtcgttgt gcttgccgcc
     3361 ggcacgcacg cacttctggc tggtggcggc gcgggtgtag gcgcgcttct ccaggccgag
     3421 gaagcaatcc ttaaactggt tcatccccgc gttggtgaac agcaaggtcg ggtcgttgcc
     3481 cggaatcagg gagctggagg caacacgggt gtgtcccttc tgttcgaaga agctgaggaa
     3541 ggcttcacgg atttctgcgc ttttcatagg aatcttccaa aaaccagcgg ccacgacaaa
     3601 gccggcaaag ggccgcatta tatcgggcct gcgctagggg gctagtgcct ttattgatag
     3661 ataacagcta ttaactgctc actattcgct caatgcgaac ggaattctgc aaaagctgcg
     3721 agcacctgat cgatatccgc agtggagata tccaggtgag tgaccatgcg cagccgtggt
     3781 gcggcgctga gtttgatccc gcgctcggcg cagaaggctt tcagcgcgtc ggcctgctcg
     3841 cccacctggc cgtagaccat attggtctgc accggctcca ccgtatagcc caactcgcgc
     3901 aaaccagctg ccaggcgctc ggcattggca tgatcctcag ccaggcgcgc cacttgatgt
     3961 tgcaaggcat acaaaccggc cgcagcgagc atgccagcct gacgcatgcc gccgccgacc
     4021 attttgcgca aacgccgcgc cctgccgatc aactcgactg agccgcacag caccgagcca
     4081 attggtgcac cgaggccctt ggacaggcac accgacaccg aatcgaagta ttgagtgatt
     4141 tcccgcgctg gcacgcccag tttgactgcg gcgttataca aacgcgcgcc gtccagatgc
     4201 agggccaggc cgcgtcgcgt ggtcagctcg cgtgccgctt gcaggtaagt ctgcggcagc
     4261 accttgccct gcatggtgtt ttccagcgca agcagccggg tgcgggcgaa gtggaagtca
     4321 tcctgcttaa tcgcggcttc gaccttggcc aagtccagtg agccatcggc ctcgccatcg
     4381 atcggctgcg gctggatcga gcccagcacc gccgcaccgc cgccttcgta tttataagtg
     4441 tgcgcctgct ggccgacgat gtattcgtcg ccgcgcgcgc aatgggccat tagcccgagc
     4501 aggttgctca tggtaccggt gggtacaaac agcgcagctt caaaaccgag ctcggcggcc
     4561 aggtactgct ccagacggtt caccgtaggg tcttcgccat acacgtcatc gcccagctcg
     4621 gcggcttgca tggcctcgcg catacctgga gtcggctggg tgacggtgtc gctgcgcagg
     4681 tcgataacgg gcatggcggg cagtccttag atggggcgaa gccccgatgc taaagcgcgc
     4741 cacttaggca aacaaggtac agatcgggcg agaaaacgca ggacagattg cgtagggtgg
     4801 atgacgtctt tttcatccac cagacacgcc ccaattggtg gatggagaaa gcgccatcca
     4861 ccctactggc tcaagccagc tgcacgcctt cggcgggaac aatcaggatg ccggcgcgca
     4921 ggccgttctt gaccttgggg ttggggaaga taatccgcgc gcccggctca tccaccaccc
     4981 agcgggtgtc ggccagatcc tcggcgagca ggtagccaat cggtaattcg ctgaagttct
     5041 cgatatcggc cggcaggtgc agcttgaaag cgtcgctgtg tttgatcact tcacgcgcca
     5101 cggcaaacaa cttcagttcg cccagatcat ccgtagcacc ctcgggctcg cggccttcga
     5161 tcagcgcttg cagatgcagt tcgagcaggt ccagattaac cagctcgttc tgcccaaacg
     5221 gccgcgcctt gcccagttcc agggtgaagg cttcagcccc cagctgggtg taggtgtagg
     5281 cgctgaaggt gatcgagctt ttattgtgca gcagcaccgc ctcaatcccg gcagcgctca
     5341 ggcgcgccag ttcacgctga ctgcgcgggc ggccttcctg aaacggataa agggcaaact
     5401 gctcgatctt cgacgcacga atggcggtgt gcaggtcgta atgcagacgg gtgcgc
//
LOCUS       DASRUM010000137         2505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1415935, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000137 DASRUM010000000
VERSION     DASRUM010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415935"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFF22_09165"
     CDS             <1..325
                     /locus_tag="VFF22_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-methylproline demethylase"
                     /protein_id="HZX17015.1"
                     /translation="MLEKVYREGDKLVAVLENEYTGAKEERVVDQVVVENGVRPDERL
                     YYALKEGSRNKGQIDVEALFAIKPQPCLSEAGDGYLLFRIGDCVAQRNTHAAIYDALR
                     LCKDF"
     gene            496..2457
                     /gene="dgcB"
                     /locus_tag="VFF22_09170"
     CDS             496..2457
                     /gene="dgcB"
                     /locus_tag="VFF22_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylglycine demethylation protein DgcB"
                     /protein_id="HZX17016.1"
                     /translation="MLNTLLPILLFAALGLAVLGAGKRFLMWRRGRPAQVDWLGGLLA
                     MPRRYMVDLHHVVARDKYIANTHVATAGGFVLAAALAILVHGFGLHNRILGFALLGAT
                     ALMFAGALFVAKRRLDPPARLSKGPWMRLPKSLLMFAASFFIATLPVAGILPEGFGGW
                     VLALLLTVGVAWGVSELFFGMTWGGPMKHAFAGALHLAWHRRSERFGGGRSTGLRALN
                     LDDPKAPLGVEKPTDFTWNQLLGFDACVQCGKCEAACPAFAAGQPLNPKKLIQDMVVG
                     LAGGTDAKFAGSPYPGKAIGEHSGGPHLPIVNLGGKALVDAETLWSCTTCRACVEECP
                     MMIEHVDAIVDMRRHLTLEKGATPNKGAEVLDNLIATDNPGGFAPGGRLNWAADLNLP
                     LMADKGSADVLFWVGDGAFDMRNQRTLRAFVKVLKAANVDFAVLGLEERDSGDVARRL
                     GDEATFQLLAKRNIATLAKYQFSKIVTCDPHSFHVLKNEYGELGGRYQVLHHSTYMDE
                     LITGQKLNLGTHKGGSVTYHDPCYLGRYNGEYEAPRNVLNAIGIEVKEMQRSGFRSRC
                     CGGGGGAPITDIPGKQRIPDMRMVDIKETGAELVAVGCPQCTAMLEGVVGVHPEVKDI
                     AELVADVLIEGVVEAKKPEPAKPELAEVQ"
BASE COUNT          438 a          762 c          832 g          473 t
ORIGIN      
        1 gatgctggag aaggtctacc gcgaaggcga caagctggtg gcggtgctgg agaacgaata
       61 caccggcgcg aaagaagagc gcgtggtcga tcaggtggtg gtggaaaacg gcgtgcgccc
      121 tgacgagcgc ctgtactacg cgctcaagga aggctcgcgc aacaagggcc agatcgacgt
      181 ggaagcgctg tttgcgatca agccgcagcc gtgtttatcc gaggcgggcg acggttatct
      241 gctgttccgt atcggcgact gcgtggccca gcgcaacacc catgcggcga tctacgacgc
      301 gctacggttg tgtaaggact tctgagccca gcacaacgtc gacggtctga cccggtctag
      361 ctccctctcc cttttgggga gagggctggg gtgagggggc ggtctcacag gccagcccct
      421 caccctaacc ctctccccgg aggggcgagg ggactgaaat gcatcatccc ctgacattgg
      481 cggtgggagc ctcacatgtt gaacaccctt cttcccattc tgctgttcgc cgcactcggc
      541 ctggccgtgc tcggcgccgg caagcgcttt ctgatgtggc gccgtggccg cccggcccag
      601 gtcgactggc tgggcggcct gctggccatg ccgcgacgct atatggtcga cctgcaccat
      661 gtggtggctc gcgacaaata catcgccaac actcacgtcg ccaccgcagg cggtttcgtt
      721 ctggcggcgg cgctggcgat tctggtgcat ggttttggcc tgcataaccg catccttggt
      781 ttcgcgctgc tgggcgccac ggcgttgatg tttgccggcg cgctgtttgt cgccaagcgt
      841 cgcctcgatc cgccggcgcg gttgtcgaaa ggcccgtgga tgcgcctgcc gaaaagcctg
      901 ctgatgttcg ccgccagctt ctttatcgct accttgccgg tggccggcat cctgccggaa
      961 ggctttggtg gctgggtgct ggcgctgctg ctgaccgtcg gcgtggcctg gggggtgtcc
     1021 gagttgttct tcggcatgac ctggggcggg ccgatgaagc acgccttcgc cggtgccctg
     1081 catctggcct ggcaccgccg tagcgaacgc ttcggcggtg gtcgttcgac cggtttaagg
     1141 gccttgaatc tggatgaccc gaaagcgccg ctgggcgtgg aaaaacctac ggacttcacc
     1201 tggaaccagc tgctcggctt cgacgcctgc gtgcagtgcg gcaaatgcga ggcggcctgc
     1261 ccggccttcg ctgccggcca accgctgaat ccgaagaagc tgattcagga catggtggtc
     1321 ggtttggccg gcggcacgga tgccaagttc gccggttcac cgtatccagg aaaagccatt
     1381 ggcgagcaca gcggcggtcc gcacctgccg atcgtcaact tgggtggcaa ggccctggtc
     1441 gatgccgaga cgctgtggtc ctgcaccacc tgccgcgcct gcgtcgagga atgcccgatg
     1501 atgatcgagc acgtcgacgc catcgtcgat atgcgccgcc atctgaccct ggagaaaggc
     1561 gcgacgccga acaagggcgc tgaggtgctg gacaacctga ttgccaccga caaccccggc
     1621 ggctttgccc cgggtggccg gctgaactgg gcggcggacc tgaacctgcc gcttatggcc
     1681 gacaagggca gcgccgatgt gctgttctgg gttggcgatg gcgccttcga tatgcgcaat
     1741 cagcgtaccc tgcgcgcctt tgtcaaagta ttgaaagccg ccaatgtcga cttcgccgtg
     1801 ctcggtttgg aagaacgtga cagcggcgac gtggcccggc gcctgggcga tgaagcgacc
     1861 ttccagctgc tggccaaacg caatatcgcc accctggcca agtaccagtt cagcaagatc
     1921 gtgacctgtg acccgcacag cttccatgtg ctgaaaaacg aatacggcga gctgggcggc
     1981 cgctatcagg tgctgcacca cagcacctat atggatgaac tgatcaccgg ccaaaaactc
     2041 aatctcggta cccacaaggg gggctcggtc acttaccacg acccatgcta cctgggccgc
     2101 tacaacggtg agtacgaggc gccacgcaat gtactgaatg ccatcggcat tgaagtgaag
     2161 gaaatgcagc gttcgggctt ccgctcccgt tgctgcggcg gtggcggcgg tgcgccgatc
     2221 accgatattc ctggcaagca gcgcatcccc gatatgcgga tggtcgatat caaggagacc
     2281 ggtgccgagc tggtggccgt cggttgcccg cagtgcacgg cgatgctcga aggtgtggtc
     2341 ggcgtgcatc cggaggtcaa ggatattgcc gagctggtgg ccgatgtatt gatcgagggt
     2401 gtggtcgagg cgaagaaacc tgagccggca aagcccgagc tggcggaggt gcagtgatgt
     2461 gtcgactaat gcgccaacgg cctgccccct cgcccctccg gggag
//
LOCUS       DASRUM010000138         8046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_580853, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000138 DASRUM010000000
VERSION     DASRUM010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8046
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580853"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1249
                     /gene="dnaJ"
                     /locus_tag="VFF22_09175"
     CDS             122..1249
                     /gene="dnaJ"
                     /locus_tag="VFF22_09175"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZX17017.1"
                     /translation="MAKRDFYEVLGVERGASEAELKKAYRRLAMKYHPDRNPDDKASE
                     EKFKEANEAYEVLSDAAKRSAYDQYGHAGVDPQMGGGGGGPGGANFSDIFGDVFSDFF
                     GGGGRGGQRGGAQRGSDLRYTLELDLEEAVRGTTVTIRVPTLVNCKPCDGSGAKKGTT
                     PVTCTTCGGIGQVRMQQGFFSVQQTCPRCHGSGKMITDPCGSCHGHGRVEEHKTLSVK
                     VPAGVDTGDRIRLSGEGEAGAMGGPAGDLYVVVNVREHAIFQRDGKHLYCEVPISFAD
                     AALGGELEVPTLDGRVKLKIPEGTQTGKMFRLRGKGVAPVRGGGAGDLMCKVAVETPV
                     HLDKRQRELLDEFRKTLEGDSSHSPKANGWFEGVKRFFGDL"
     gene            1321..2124
                     /gene="dapB"
                     /locus_tag="VFF22_09180"
     CDS             1321..2124
                     /gene="dapB"
                     /locus_tag="VFF22_09180"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017277904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HZX17018.1"
                     /translation="MQRIAVMGAAGRMGKTLIEAVQLVDGASLSAAIDRADSSLVGAD
                     VGELVAQGKIGVNLSGDLNAVLDQFDVLIDFTHPSVTLKNLEICRQAGKSMVIGTTGF
                     SPEEKQLLAEAGKQISIVFAANFSVGVNLCLKLLDTAARVLGDDVDIEIIEAHHRHKV
                     DAPSGTALRMGEVVANALGRDLQKVAVYGREGQTGARERETIGFATVRAGDVVGDHTV
                     LFAADGERVEITHKASSRMTFAKGAVRAALWLQNRKPGLYDMQDVLGLN"
     gene            2334..3482
                     /gene="carA"
                     /locus_tag="VFF22_09185"
     CDS             2334..3482
                     /gene="carA"
                     /locus_tag="VFF22_09185"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HZX17019.1"
                     /translation="MSKPALKPAILALADGSIFRGEAIGADGQTIGEVVFNTAMTGYQ
                     EILTDPSYAQQIVTLTYPHIGNTGTTPEDAESNRVWAAGLIIRDLPLTSSNWRNKQPL
                     DEYLKENGTVAIAGIDTRRLTRILREKGSQNGCILVGDDATEEKALELARSFPGLKGM
                     DLAKVVSCTERYEWRSSVWNLQDDSHPEIPASELPHHVVAYDYGVKWNILRMLVERGC
                     RVTVVPAQTPASDVLALNPDGVFLSNGPGDPEPCDYAIAAIKDVLETDIPVFGICLGH
                     QLLALASGAKTLKMGHGHHGANHPVQALKTGVVMITSQNHGFAVDESSLPSNVRAIHK
                     SLFDGSLQGIERTDKDAFSFQGHPEASPGPADVAPLFDRFIEAMAKRR"
     gene            3519..6740
                     /gene="carB"
                     /locus_tag="VFF22_09190"
     CDS             3519..6740
                     /gene="carB"
                     /locus_tag="VFF22_09190"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HZX17020.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALKEEGYRV
                     ILVNSNPATIMTDPAMADATYIEPIKWSTVAKIIEKERPDALLPTMGGQTALNCALDL
                     EKHGILEKFGVEMIGANADTIDKAEDRSRFDKAMKDIGLACPVSGIAHNMEEAYGVLE
                     KVGFPCIIRPSFTMGGTGGGIAYNREEFEEICARGIDLSPTSELLIDESLIGWKEYEM
                     EVVRDKKDNCIIVCAIENFDPMGVHTGDSITVAPAQTLTDKEYQILRNASLAVLREIG
                     VETGGSNVQFGICPNTGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     LSNDITGGRTPASFEPAIDYVVTKIPRFAFEKFPKADARLTTQMKSVGEVMAIGRTFQ
                     ESLQKALRGLEVGVSGLDPMLDLADPEAESTLRRELTVPSADRIWYIGDAFRAGKTVA
                     EVFELTNIDEWFLVQIEDLIKDEERIKTLALSSIDRDSMYKLKRKGFSDIRLAKLLGV
                     TEKTLRSQRHKLKVLPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTRDKIMILGG
                     GPNRIGQGIEFDYCCVHAALALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLED
                     VLEIVRVEQPKGVIVQYGGQTPLKICRALEEAGVPIIGTSPDAIDRAEDRERFQQMVQ
                     RLGLRQPANATARSEDEALAHSKTIGYPLVVRPSYVLGGRAMEIVYQEDELKRYMREA
                     VKVSNDSPVLLDHFLNCAIEVDVDAVCDGETVVIGAIMQHIEQAGVHSGDSACSLPPY
                     SLPMHIQDEIREQVKKMALELDVVGLMNVQMAVQGEDIFVIEVNPRASRTVPFVSKCV
                     GVSLAKVAARVMAGKTLAEAGYTAEIIPPYFSVKEAVFPFAKFPGVDPILGPEMKSTG
                     EVMGVGDTFGEAFAKAQSGASEILPNAGVAFISVRDDDKPLVAGVARDLIELGFEVVA
                     TAGTAKLIEAAGLPVRRVNKVTEGRPHVVDMIKNDEVTLIINTTEGRQSIADSYSIRR
                     NALQHKIYCTTTIAAGQAICEALKFGPEKTVRRLQDLHAGINA"
     gene            6737..7213
                     /gene="greA"
                     /locus_tag="VFF22_09195"
     CDS             6737..7213
                     /gene="greA"
                     /locus_tag="VFF22_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HZX17021.1"
                     /translation="MIKYPMTVQGARALEEEHAHLTKVVRPKLSQDIGTARELGDLKE
                     NAEYHAAREQQGMVEARIRDIEGRLQNAVIIDVTTIEHTGKVIFGTTVEIANCETDES
                     VTYQIVGEDEADIKQRKISVGSPIARALIGKSEGDVVVVQTPSGALEYEIVEVRHL"
     gene            7185..7631
                     /locus_tag="VFF22_09200"
     CDS             7185..7631
                     /locus_tag="VFF22_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HZX17022.1"
                     /translation="MRLSRFAISKPLVRDAGAISWLLAQTLWVGGLWLLHFLLLPAIA
                     KLGLASLLVEEIGNTLRPLLVGLAAVCAGLQVLVLIRAERLASLWRDTRGQLLLVVVA
                     MAGSYFAVRQLLPEAELWLMFSYLVMAFCGLLLVLQPLPQKLRHKP"
     gene            complement(7698..8009)
                     /gene="yhbY"
                     /locus_tag="VFF22_09205"
     CDS             complement(7698..8009)
                     /gene="yhbY"
                     /locus_tag="VFF22_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005888980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HZX17023.1"
                     /translation="MPLTQEQKKQFKSIGHHLKPVLIVADNGLTEGVLTELERALSDH
                     ELIKVQLRITEREDRLTAINELCKAGKAELVQVIGKMALIYRKNPKVNKNLSNVHRHH
                     G"
BASE COUNT         1648 a         2290 c         2477 g         1631 t
ORIGIN      
        1 cgcaagctta tgccgctctg gccgggcgtg acgcgagtcg cgttcggcat gatccgccgc
       61 gcgggagctt gctcccgcgt tggcgtgtct ggagtagacg atttaaaggt gttgaagact
      121 tatggctaaa cgcgattttt acgaggtgct cggtgtcgag cgcggtgcca gcgaagcgga
      181 gctgaaaaaa gcctaccgcc gtctggccat gaagtatcac ccggaccgta accctgacga
      241 taaggcctcg gaagagaagt tcaaggaagc caacgaggct tacgaagtgc tctccgatgc
      301 ggccaaacgt tcggcttacg atcagtacgg gcacgccggt gttgacccgc agatgggtgg
      361 cggcggtggc ggcccgggcg gggcaaactt ctccgatatc ttcggcgatg tattcagcga
      421 tttcttcggc ggcggtggtc gcggcggtca acggggcggc gcacagcgcg gcagtgatct
      481 gcgctacacc ctggagctgg acctggaaga agcggtgcgt ggcactacgg tgaccattcg
      541 cgtaccgacg ctggtcaatt gcaagccttg cgatggctcg ggcgcgaaga agggcaccac
      601 gccggtgacc tgtaccacct gtggcggtat cggccaggtg cgtatgcagc aaggcttctt
      661 ctcggtgcag cagacctgtc cgcgctgcca cggcagcggc aagatgatca ccgatccttg
      721 cggctcctgc cacggccatg gccgggtaga ggagcacaag actctgtcgg tgaaagtgcc
      781 ggctggcgtc gataccggcg accgcattcg tctatctggc gaaggcgagg cgggtgccat
      841 gggcggtccg gcgggcgatc tgtacgtggt ggtgaatgtg cgcgagcacg cgatcttcca
      901 gcgtgatggc aagcacctgt actgcgaagt gccgatcagc tttgccgatg cggcgctggg
      961 gggcgagttg gaagtgccga ccctggatgg tcgggtcaag ctgaagattc cggaaggtac
     1021 gcagaccggc aagatgttcc gtctgcgtgg caagggcgtt gccccagtgc gtggcggcgg
     1081 tgccggcgac ctgatgtgca aggtggcggt ggaaaccccg gtgcacctgg ataaacgcca
     1141 gcgcgaactg ctcgatgagt tccgtaaaac cctggaaggc gacagctcgc attcgcctaa
     1201 ggccaatggc tggttcgaag gcgttaagcg cttctttggc gacctgtaaa tcccgctgac
     1261 aggctgcgcg gtcgctaagc ggcggctgca gcctgtggcg acagcttgga gctgatagct
     1321 atgcaacgta ttgctgtgat gggcgccgct gggcgcatgg gcaagaccct gatcgaagcc
     1381 gtgcagctgg tcgatggcgc aagcctgagc gcggcgattg atcgtgccga tagcagcctg
     1441 gttggcgcgg atgtcggtga gctggtggcg cagggcaaga ttggcgtcaa tctgtccggc
     1501 gatctgaatg cggtgcttga tcagttcgat gtgctgatcg acttcactca tccatcggtc
     1561 accctaaaga acctggaaat ctgccgccag gccggtaagt ctatggtgat tggcactacc
     1621 ggtttttccc cggaagaaaa gcagctgttg gctgaggccg gcaagcagat ttccatcgtg
     1681 tttgccgcca acttcagtgt cggcgtcaat ctgtgtctga agctgctcga caccgcggca
     1741 cgggttttgg gcgatgacgt ggatatcgaa atcatcgaag cccatcaccg tcacaaggtc
     1801 gatgcgccat ccggcaccgc cttgcgtatg ggggaagtag tggctaatgc cctggggcgt
     1861 gatctgcaga aagtcgcggt gtatggccgt gaagggcaga ccggtgcgcg cgagcgcgaa
     1921 accatcggtt ttgccacggt gcgcgccggt gatgtggtgg gtgatcacac cgtgttgttc
     1981 gccgccgatg gcgagcgcgt ggaaatcacc cacaaggcat ccagccgtat gaccttcgcc
     2041 aaaggcgcgg tgcgcgctgc gttgtggctg cagaatcgta agccgggcct gtacgacatg
     2101 caggacgttc tgggtctcaa ctgaggcatt gtgtcgcagg aagggctttt aactgggtca
     2161 tagtcgcaac tggcggtgga ccaaaaaggc agtttcctgt aagctttctg ctttagtgtg
     2221 tccactaaag ttgcgcagaa tgaatcagat aaaaaagcgg ggtgacggtt gatacgtcat
     2281 cccgcttttt tacaatctgc gtttgcttca cgttttgatt tttggaggtt ttcttgagta
     2341 agccagccct taaacctgcc attttggctc ttgctgatgg cagtattttt cgcggcgagg
     2401 ccatcggtgc cgatggccag actattggtg aggtggtttt taacaccgcc atgaccggct
     2461 atcaggaaat ccttaccgat ccttcctatg cccagcagat tgtcaccctg acctatccgc
     2521 acatcggcaa caccggcacc acgccggaag acgccgagtc caaccgcgta tgggccgccg
     2581 gcctgattat ccgtgacctg ccgctgacct ccagcaactg gcgcaacaag cagccgctgg
     2641 atgaatacct caaggaaaac ggtaccgtcg ccatcgccgg catcgatacc cgccgcctga
     2701 cccgtatttt gcgtgagaaa ggctcgcaaa acggctgcat tctggttggc gacgacgcca
     2761 ccgaagagaa ggcgctggaa ctggcgcgca gcttccctgg ccttaaaggt atggatttgg
     2821 ccaaagtggt cagctgcacc gagcgttacg aatggcgctc cagcgtgtgg aacctgcaag
     2881 acgacagcca tccagaaatc ccggccagcg agctgccgca tcacgtggtt gcctacgatt
     2941 acggcgttaa gtggaacatc ctacgcatgc tggtcgagcg cggttgccgc gtgactgtgg
     3001 tgcctgcgca gactccagct agcgatgtgc tggcgctgaa tcccgatggc gtgttcctgt
     3061 ccaacggccc gggcgatccc gagccgtgtg actacgccat cgcggcaatc aaggacgtgc
     3121 tggaaaccga tatcccggta ttcggcatct gcctcggcca ccagttgctg gccctggcct
     3181 ctggtgccaa gaccctgaaa atgggccacg gtcaccacgg tgccaaccat ccggtgcagg
     3241 ccctgaaaac cggcgtggtg atgatcacta gccagaacca cggtttcgcc gtggatgaaa
     3301 gcagcctgcc aagcaacgtg cgcgccattc acaaatcgct gttcgacggt tccctgcagg
     3361 gtatcgaacg gaccgataag gacgccttca gcttccaggg tcaccccgaa gcgagccccg
     3421 gcccagccga tgtggcaccg ctgttcgacc gttttatcga agccatggcc aagcgccgtt
     3481 aagcccgccg actgacccaa ggattcgaga gagagaacat gccaaagcgt acagacatta
     3541 aaagcatcct gatcctcggc gctggcccga ttgttatcgg ccaggcctgc gagttcgact
     3601 attccggtgc ccaggcgtgt aaagcgctga aagaagaggg ctaccgcgtc attctggtga
     3661 actccaaccc ggccaccatc atgaccgacc cggccatggc ggacgccacc tatatcgaac
     3721 cgatcaagtg gtccaccgtg gccaagatca tcgagaagga gcgcccagac gcgctgctgc
     3781 caaccatggg tggccagacc gcgctgaact gcgcactgga cctggaaaag cacggcatcc
     3841 tggaaaaatt tggcgtcgag atgatcggtg ccaatgccga caccatcgac aaggccgaag
     3901 accgctcgcg cttcgacaag gcgatgaaag acatcggtct ggcctgcccg gtttctggca
     3961 tcgcccataa catggaagaa gcctacggcg tgctggagaa ggttggcttc ccctgcatca
     4021 tccgtccgtc cttcaccatg ggtggcactg gcggcggtat tgcttacaac cgtgaagagt
     4081 tcgaagaaat ctgcgctcgc ggtatcgatc tgtcgccgac tagcgaactg cttatcgatg
     4141 aatcgctgat cggctggaaa gagtacgaga tggaggtggt ccgcgataaa aaggacaact
     4201 gcatcatcgt ctgcgccatt gaaaacttcg acccgatggg cgtgcacacc ggcgactcga
     4261 tcaccgtggc tccggcacaa accctgaccg acaaggaata ccagatcctg cgtaacgcct
     4321 ccctggcggt actgcgtgag atcggcgtgg aaaccggcgg ctccaacgtg caattcggca
     4381 tctgcccgaa cactggccgt atggtagtga tcgagatgaa cccgcgcgtg tcgcgttctt
     4441 cggcgctggc ctccaaggcc accggcttcc cgatcgccaa gatcgccgcc aaactggcgg
     4501 tgggttacac cctcgacgag ctgtccaacg acatcaccgg cggtcgcacc ccggcctcgt
     4561 tcgagccagc cattgactac gtcgtgacca agatcccgcg ttttgccttc gagaaattcc
     4621 cgaaagctga tgcccgtctg accacccaga tgaagtccgt cggtgaagtt atggccatcg
     4681 gccgcacctt ccaggaatcc ctgcagaaag ccctgcgcgg tctggaagtg ggcgtttcgg
     4741 gcctcgatcc gatgctcgat ctggccgatc ctgaagccga aagcaccctg cgtcgcgagc
     4801 tgaccgtgcc aagcgctgac cgtatctggt acatcggcga tgccttccgt gccggcaaga
     4861 ccgtggccga agtcttcgag ctgaccaata tcgatgaatg gttcctggtg cagatcgaag
     4921 atctgatcaa ggacgaagag cggatcaaga cgctggcatt gtcgagcatc gaccgtgatt
     4981 cgatgtacaa actcaagcgt aaaggcttct ccgatattcg cctggccaag ctgctgggcg
     5041 tgaccgagaa gaccctgcgt agccagcgcc acaagttgaa agtgctgccg gtgtacaagc
     5101 gcgtcgacac ctgcgctgcc gagttcgcca ctgacactgc ttacatgtac tcgacctatg
     5161 aggaagagtg cgaagccaat ccgtcgaccc gcgacaagat catgatcctc ggtggcgggc
     5221 cgaaccgtat cggccagggc atcgagttcg actactgctg cgtacatgca gcactggcac
     5281 tgcgtgaaga cggttacgag accatcatgg tcaactgcaa cccagagacc gtctccaccg
     5341 actacgacac ctccgaccgt ctgtacttcg agccggtaac cctggaagac gtgctggaaa
     5401 tcgtccgtgt tgagcagccg aaaggcgtga tcgtgcagta cggcggccag acccctctga
     5461 aaatctgccg cgccctggaa gaagccggcg tgccgatcat cggtacctcg cctgatgcca
     5521 tcgaccgcgc cgaagaccgc gagcgcttcc agcagatggt tcagcgtctc ggcctgcgtc
     5581 agccggctaa cgccaccgcg cgcagcgaag acgaagccct ggcccactcc aagaccatcg
     5641 gttatccgct ggtggtgcgt ccttcctatg ttctgggcgg ccgtgctatg gaaatcgtct
     5701 atcaggaaga cgagctcaag cgttatatgc gcgaagcggt aaaagtctcc aacgacagcc
     5761 cggtgctgct cgatcacttc ctcaactgcg ccatcgaagt ggacgtggat gcggtgtgcg
     5821 acggcgagac tgtggtgatt ggcgcgatca tgcagcacat tgagcaggct ggtgtgcact
     5881 cgggtgactc cgcctgttcg ctgccgccgt actcactgcc aatgcacatc caggacgaga
     5941 tccgtgagca ggtcaagaaa atggccttgg agctcgacgt ggttggcctg atgaacgtgc
     6001 agatggccgt gcagggtgag gacatcttcg ttattgaagt gaacccgcgc gcctcgcgta
     6061 ccgtaccgtt tgtctccaag tgcgtcggcg tttcgctggc caaggtggct gcgcgcgtga
     6121 tggccggcaa aactctggct gaagccggct acaccgccga gatcattccg ccgtacttca
     6181 gcgtcaagga agcggtgttc ccgttcgcca agtttccagg cgtcgacccc atcctcggcc
     6241 cagaaatgaa gtccaccggt gaagtgatgg gcgtcggcga cacgttcggt gaagccttcg
     6301 ccaaggcaca gtcgggggcc agtgagattc tgccaaacgc cggcgtggcc tttatcagcg
     6361 tgcgtgacga tgacaagccg ctggttgctg gcgtggcgcg tgacctgatt gaactgggct
     6421 ttgaagtggt ggccactgcc ggtaccgcca agctgatcga ggcggcgggt ttgcccgtgc
     6481 gtcgcgtcaa caaggtaacc gagggtcgcc cgcacgtggt cgacatgatc aagaatgatg
     6541 aagtcactct gattatcaac accaccgaag gtcgtcagtc gattgctgac tcctattcca
     6601 ttcgtcgtaa cgctctgcag cacaagatct actgcaccac caccattgcg gcggggcagg
     6661 caatctgtga ggcgctgaag ttcggtcccg agaagactgt gcgccgtctg caggatctgc
     6721 atgcaggaat caacgcatga tcaaataccc aatgaccgtc cagggcgcgc gcgccctgga
     6781 agaggagcac gctcacctga ccaaggtggt gcgtcccaaa ctgagccagg atatcggcac
     6841 tgcgcgcgag ctcggtgacc tcaaagagaa cgctgaatac catgccgcgc gcgagcagca
     6901 gggtatggtc gaggcgcgta tccgcgatat cgagggtcgt ctgcagaatg cggtgatcat
     6961 cgacgtcacc acgattgaac acacgggcaa ggtgattttc ggtacgaccg tggaaatcgc
     7021 aaactgcgaa accgatgaaa gcgtcactta ccagatcgtg ggtgaggacg aagctgacat
     7081 caagcagcgc aagatttctg taggctcacc tatcgcccgc gccttgatcg gcaagagtga
     7141 gggggatgtg gtcgtggtgc agacgccgag cggtgcgctg gagtatgaga ttgtcgaggt
     7201 tcgccatctc taagccgctg gtgcgtgacg ctggcgcaat cagctggctg ctggctcaga
     7261 cgctctgggt tggcggcctc tggttgctac attttttgct gctgcctgca attgccaagc
     7321 tcggcctggc ctcattgcta gtggaagaaa tcggcaatac cttgcggcca ttgctggttg
     7381 gcctggcggc ggtttgcgcc ggactccagg tgctggtact gattcgtgcc gaacgcctgg
     7441 caagcctgtg gcgtgatacc cgcgggcaat tgctattggt tgtagtagcg atggcgggca
     7501 gttactttgc tgtgcgccag ctgctgccag aggctgaact gtggttgatg ttcagttact
     7561 tggtcatggc cttttgcggc ctgttgctgg tgttgcagcc gctgccgcaa aagctcaggc
     7621 acaagcccta agcagtaaga ttgggcagga taggtgggtt gcaaaggcgc tgagcgcgcc
     7681 ttgcaacggc tgatagatca gccgtgatga cggtgaacgt tggaaaggtt cttgttcact
     7741 ttcgggttct tgcggtagat caacgccatt ttgccaatca cctgcaccag ctcggctttg
     7801 ccggctttgc acagttcatt gatggccgta aggcgatctt cgcgctcagt gatgcgtaat
     7861 tgcactttaa tcagttcatg atcgctcagt gcgcgttcca gttcagtcag cacaccttcg
     7921 gtcagaccgt tgtcagccac gatcaataca ggcttcaaat ggtgaccgat agatttgaat
     7981 tgtttcttct gctcttgagt gagcggcata atctgattcc tgcatttaat ctggtaaaaa
     8041 acggcg
//
LOCUS       DASRUM010000139        14621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1078231, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000139 DASRUM010000000
VERSION     DASRUM010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 14621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14621
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078231"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VFF22_09210"
     CDS             <1..767
                     /locus_tag="VFF22_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZX17024.1"
                     /translation="LFPAWLIVLYSLPLLGIWWVLAHIDGNLAALFAGSMLLGYMSYE
                     VVHACEHLPAEHPLTRLPWIRQMRRLHALHHRRELMHARNFGIVHPLMDWLYGTLHWE
                     AEPIYQQNRQQHRVHIARPVDEVLSYTAAPIHWPEWHPSSLHIYGRDGVLLAGDVFEE
                     DIHAGGRAGHLRWDVLDYQPARRWQASARGDHGLGLLLTYECLEVEGGCEFVRTLEYG
                     FDGVLMRLANRLLLSRRIERESQASMQALRAVLERS"
     gene            complement(784..1536)
                     /gene="cysZ"
                     /locus_tag="VFF22_09215"
     CDS             complement(784..1536)
                     /gene="cysZ"
                     /locus_tag="VFF22_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007970299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HZX17025.1"
                     /translation="MPAPVLTGPQYLSEGLKLVLSPGLRLFVLLPLTINLILFIGLIY
                     LAMQQFGGWVDTFMPSLPSWLSFLEYVLWPLFVVLVLLMVFFTFTLLANIIAAPFNGF
                     LAEKVEVVVRGEDPFPAFRWGELIEMVPRTLGREMRKLGYFLPRAIALLILTFIPVVN
                     LIAAPLWLLFGVWMMAIQYIDYPADNNKMSWQDMLAWLREKRWQSLGFGGITYAALLV
                     PGLNILMMPAAVAGATVFWVRERQEQALTSTS"
     gene            1815..2762
                     /gene="trxB"
                     /locus_tag="VFF22_09220"
     CDS             1815..2762
                     /gene="trxB"
                     /locus_tag="VFF22_09220"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HZX17026.1"
                     /translation="MSATRHARLIILGSGPAGYSAAVYAARANLKPLLITGLQAGGQL
                     TTTTEVDNWPGDVHGLTGPALMQRMQEHAERFETEIVYDHINAVDLAGKLFTLVGDSA
                     TYSCDALIIATGASARYLGLPSEESFMGKGVSACATCDGFFYRNREVAVVGGGNTAVE
                     EALYLANIASKVTLVHRRGSFRAEKILQNKLQARVAEGKIVLKLHAEVDEVLGNNMGV
                     TGVRVKNNDGSFDELSVEGLFVAIGHTPNTSLFEGQLALKDGYLVVNGGRDGNATATS
                     VPGVFAAGDVADHVYRQAITSAGAGCMAALDVERYLDGQ"
     gene            complement(2944..3531)
                     /locus_tag="VFF22_09225"
     CDS             complement(2944..3531)
                     /locus_tag="VFF22_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HZX17027.1"
                     /translation="MQQTLVWKPWHNPGVENLRLNIDEHGINATSHLMQSLQGHSIAA
                     TYVLNCDPRWRFRRLWLKVDNHGQRSLNLKRDIRGNWFHNGEPRPDLSECQQVMLSES
                     PFTHTPALQRCALETGQSELMQVAYVDLLTLKVEARSQRYQRLREEGATTLYRSEAQG
                     KKSSELTVDEHALLVKASDQFLRMSSRDLKLNTWV"
     gene            complement(3790..4134)
                     /locus_tag="VFF22_09230"
     CDS             complement(3790..4134)
                     /locus_tag="VFF22_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HopJ type III effector protein"
                     /protein_id="HZX17028.1"
                     /translation="MSSLNDFRAKLRGEHFTFAETLAFIAEHYQYQPSAFSNGNVANT
                     AGQNEGSCKTLGLALLEGLSLEETLLCFGEHYRSVLATPSGSDHGNIRALMETGLADV
                     RFEQPSLQRNSL"
     gene            complement(4134..4478)
                     /locus_tag="VFF22_09235"
     CDS             complement(4134..4478)
                     /locus_tag="VFF22_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HZX17029.1"
                     /translation="MLLSSPPTPPRLPLESIAMTEQERLELEAAAFRSLVQHLRSRSD
                     VQNIDMMNLAGFCRNCLSKWFKAAADDIGVAITPDEAREEVYGMPYAEWKAKYQKEAS
                     ADQQAAFSKGKK"
     gene            complement(4549..4920)
                     /gene="folX"
                     /locus_tag="VFF22_09240"
     CDS             complement(4549..4920)
                     /gene="folX"
                     /locus_tag="VFF22_09240"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HZX17030.1"
                     /translation="MPRLEPGMARIRVKDLRLRTYIGIKEEEINNKQDVLINLTILYP
                     AIEAVRDNDIDHALNYRTITKAVIRHVEENRFALLERMTQEILDLVMANPDVCYAEVE
                     VDKLHALRFAESVSITLAGHR"
     gene            complement(4920..5480)
                     /gene="folE"
                     /locus_tag="VFF22_09245"
     CDS             complement(4920..5480)
                     /gene="folE"
                     /locus_tag="VFF22_09245"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HZX17031.1"
                     /translation="MTPELPDHYREILLGVGENPEREGLLDTPKRAAKAMQYLCNGYH
                     KSLEEVVNGALFESDNDEMVIVRDIELYSLCEHHMLPFIGKAHVAYIPTGKVLGLSKV
                     ARIVDMYARRLQIQENLTKQIADAIQQVTNAAGVAVVIEAKHMCMMMRGVEKQNSVMT
                     SSVMLGAFRESCNTRHEFLQLIGRSK"
     gene            complement(5503..6213)
                     /gene="folM"
                     /locus_tag="VFF22_09250"
     CDS             complement(5503..6213)
                     /gene="folM"
                     /locus_tag="VFF22_09250"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HZX17032.1"
                     /translation="MSVSAAPILITGASQRIGLYCAERLLDDGHPVIVTYRSERDSID
                     ALRQRGAIALQADFASEAGILAFIEQLKSHTDSLRAIVHNASDWLMEEPGEEAAAFQQ
                     LFSVHMLAPYLINLHCQALLARSTPADIVHISDDVARKGSAKRPAYCASKAGLDSLTL
                     SFAARFAPQIKVNGIAPALIMFNPDDDANYRNKTLNKSALGIEPGPQVIYQSLRYLLD
                     NPYVTGTTLTVNGGRHLK"
     gene            6478..6774
                     /locus_tag="VFF22_09255"
     CDS             6478..6774
                     /locus_tag="VFF22_09255"
                     /inference="COORDINATES: protein motif:HMM:NF039469.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6482 family protein"
                     /protein_id="HZX17033.1"
                     /translation="MKPWQLSIAVLRGHVNHVEVRTLRCCGYKVQVVSKTGSVRTLEY
                     ASGRPLWPGLALLKACIRRCGVQQMLLLQSESHDEIIGRPALLEPDPGLWIKLR"
     gene            complement(6779..7204)
                     /locus_tag="VFF22_09260"
     CDS             complement(6779..7204)
                     /locus_tag="VFF22_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017479549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HZX17034.1"
                     /translation="MNKQPCWPLTLYYDGDCPLCAREIRLLRQRADVQRLCLVDIGLN
                     DFHPESVGHSRETLQNRLHARFADGQWVTGLDATLWSWRAAGLERWATPLTWPALRPV
                     LELGYRLFCRLRPHLDWLPHPDASRRCKNAACAVSKKPQ"
     gene            7296..8249
                     /locus_tag="VFF22_09265"
     CDS             7296..8249
                     /locus_tag="VFF22_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZX17035.1"
                     /translation="MSDHVGTASLVSNALKQEELFPIREVARLTGVNPVTLRAWERRY
                     GLIQPTRTDSGHRLYSQVDIESVRSILAWIERGVAVSKVGKILAKSSSIKSASSPVYE
                     EVSASEWGEWQAQLRRAISAFDELRLDRLYGQIFSSYPLPVVFQDILMPLWQELLLRQ
                     DEYGQTSEWLFLDTFLRARTLQRLQVLRAQVEERVLLAALPGHCRELELLVAGLLLSS
                     ADTAVSVLGLGQPLEELTLICEKMQPQALVLFSNQPPSDDLPKVLSRLALALDCPLVM
                     AGDGADLAEESLSGSPIACLGNEGRLMQRRLQQFLAGHLDT"
     gene            8457..10235
                     /locus_tag="VFF22_09270"
     CDS             8457..10235
                     /locus_tag="VFF22_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HZX17036.1"
                     /translation="MTQYRAPLRDMRFVFDEVLDAYATLQSLPSQREFGDDLGGAILE
                     EAAKLAENVLAPLNGPGDKQGCHYDPESKSVKAPDGFRAAYQQFAEGGWTALACTPEF
                     GGQGLPHVLNMMVEEMVCSANLSLGMYPGLTHGAINALTAHGTRELQERYLPKLISGE
                     WTGTMCLTEPQCGTDLGLIRTRAVPQADGSYAISGTKIWITGGEHDLVDNILHLVLAK
                     LPDAPDSVKGISLFLVPKFLADGSRNPAFCGGLEHKMGIKGSATCVMNFEGAQGWLIG
                     EPNKGLKCMFTMMNSARLMVGMQGLGIAESAYQVSLGFAKERLQSRSLSGPKAADKPA
                     DPIIVHPDVRRMLLRQKVMIEGCRALAYFTGLHEDVAHDHEDPVVREQADDLVQLLTP
                     VVKSFLTDEGFNCANEGLQVLGGSGFTEDWGIEQLVRDSRITRIYEGTNGIQALDLVG
                     RKLGLGGGRAVRNYFALVEGWLKANAEAEHVAAVGAALKQLQQATLWIASEGMKDPEQ
                     AGAAATPYLRLFALTSLAWMWARMAAVAKAKLAAGSTETLFYGAKLKSADFFMARILT
                     ETDSLLAEVKAGKATLMAFSEEEFAA"
     gene            complement(10386..10997)
                     /locus_tag="VFF22_09275"
     CDS             complement(10386..10997)
                     /locus_tag="VFF22_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17037.1"
                     /translation="MQEQALLTQAELAFIRQLHQPSTTEQRSQPHLSVDIGKQLSELL
                     SHCAVDEQLSLHAHIANQHLTFDLHLSRDEQNTPCLQLSAPQIFEEGEVNRAWRSPLA
                     EPLPLHTPSAQPSDLWIHQLSMNGALIEHRARRKAPKRFQLILPVDELTPIAVAGVFV
                     RETEDGLLAYQLHALDPHSDEQLRQFIYLQHLQQEQRLQAGRR"
     gene            complement(11049..11510)
                     /locus_tag="VFF22_09280"
     CDS             complement(11049..11510)
                     /locus_tag="VFF22_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017130703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZX17038.1"
                     /translation="MINAKLLQLVINASNDGIVVAEQEGDDNILIYANPAFEKLTGYA
                     SEDILYQDCRFLQANDRDQPGLAAIREAVKAHRPCRQIIRNYRKDGTPFWNELSITPV
                     VNDADQLTYYIGIQKNVTEQVEAQERVKQLEAELASLKAELARLQPTTGSN"
     gene            11623..12075
                     /locus_tag="VFF22_09285"
     CDS             11623..12075
                     /locus_tag="VFF22_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HZX17039.1"
                     /translation="MKIAILSGSVYGTAEEVARHAERLLKAAGHEAWHNPRASLADIQ
                     AFAPEAFLAVTSTTGMGELPDNLQPLYYAIRDHLPAWNGLPGGVIALGDSSYGDTFCG
                     GGELVRELYAELGIREVQPMLRLDSSESVTPESDAEPWLADFITALQG"
     gene            12236..13018
                     /locus_tag="VFF22_09290"
     CDS             12236..13018
                     /locus_tag="VFF22_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZX17040.1"
                     /translation="MTVAHALPVVSLKDQVSAAEWQTRVDLAACYRLIALYGWDDLIF
                     THISAKIPGSEDFLINPYGLMFHEISASSLLKVDLAGNKLMDSPFDINPAGYTIHSAV
                     HEVRHDVGCVLHIHTPQGIAVSAQKQGLLPLSQQSLFVLASLAYHGYEGVALNHDEKS
                     RLQADLGDKNFMILPNHGLLTAAGSIADAFLMMFILQRACEIQVMAQSGGAELIHIPQ
                     QVLDGARAMIAGVMKSPQGMGGALPWPALLRKLDQQMPGYAA"
     gene            13015..13926
                     /locus_tag="VFF22_09295"
     CDS             13015..13926
                     /locus_tag="VFF22_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003302968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX17041.1"
                     /translation="MSALPGIALDAWRAQGRDFSFNGQPIRYWTAGDPAAEPLLLIHG
                     FPTASWDWHYLWAPLAARYRLIACDMLGFGDSAKPRGHDYSLLEQADLQQALLAHLGE
                     SRPLHVLAHDYGDSVAQELLARHQDGRIALASCVFLNGGLFPETHHPVLVQKLLLSPI
                     GPLIGRLFSRKKLAASFAQVFGPQTQPSARELDDFWSLIAANNGPAVMHRLIRYILDR
                     RVNRDRWVAAMQSTAVTMRVIDGAVDPISGAHMVARYRELIPQPDTVLLEQIGHYPQT
                     EAPDQVLAHYLQFRERLEAAICSDALS"
     gene            13905..14435
                     /locus_tag="VFF22_09300"
     CDS             13905..14435
                     /locus_tag="VFF22_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HZX17042.1"
                     /translation="MQRRTVLKGAALGGVAVLGAGFWALPSGEAPAALSLDGAQQVLA
                     GLVDKPLSSLKGWSPTEVFNHCAQSIEYSISGYPELKPGWFRSSVGLLAFSVFAARGA
                     MRHPLDEAIPGAAPLNTPATQAQALQRLQQAFADFAAYQGELQPHFAYGALSHADYAQ
                     AHVLHLYNHLSLIRPA"
BASE COUNT         2601 a         4209 c         4665 g         3146 t
ORIGIN      
        1 tcctctttcc ggcctggctg atcgtgctct acagcctgcc gctgctgggc atctggtggg
       61 tgctggcgca tatcgacggc aacctcgccg cgctgttcgc tggcagcatg ctgctgggct
      121 atatgagcta cgaggtggtg catgcctgtg agcatctgcc ggccgagcat ccgcttacgc
      181 gattaccgtg gattcgccag atgcgccgtc tgcatgcgct gcaccatcgc cgtgagctga
      241 tgcatgcgcg caacttcggc atcgtccatc cactgatgga ctggctgtac ggcaccctgc
      301 actgggaagc ggagcccatt tatcagcaga atcgccagca gcatcgcgtg catatcgccc
      361 gcccggtgga cgaggtgctg agttataccg ccgcgcctat tcactggccc gaatggcacc
      421 cgtcgtcgtt acatatctac ggccgcgatg gtgtgttgct cgccggcgat gtgtttgagg
      481 aagacatcca cgccggtggc cgcgccggcc atctgcgctg ggatgtgctc gactaccaac
      541 cggcccgccg ctggcaggcc agcgcccgtg gcgatcacgg tttagggttg ctgctgacct
      601 acgagtgctt ggaagtggag ggcggttgcg agttcgtgcg caccctggaa tacggcttcg
      661 acggtgtgct gatgcgcctg gccaaccgcc tgctgctgag ccggcggatt gagcgcgagt
      721 ctcaagcatc catgcaggcg ctgcgcgcgg ttcttgagcg cagctagcct gcgggatgat
      781 tacttaggac gtggacgtga gcgcctgctc ctggcgttcg cgcacccaga acaccgtcgc
      841 gccggctact gccgccggca tcatcaggat attcagccct ggcaccagca aggcggcgta
      901 ggtgatgccg ccgaagccca ggctctgcca gcgtttttcc cgcagccagg cgagcatgtc
      961 ctgccagctc atcttgttgt tgtccgccgg gtagtcgatg tactgaatcg ccatcatcca
     1021 tacgccaaat agcagccaca gcggtgcggc gatcaggttg accaccggaa taaaggtcag
     1081 gatcagcagg gcgatggcgc gcggcagaaa gtagcccagc ttgcgcattt cgcggcccag
     1141 ggtgcgcggc accatttcaa tcagttcgcc ccagcggaag gcagggaaag ggtcttcgcc
     1201 gcgcaccacc acctcgacct tctccgcgag aaagccgtta aacggtgcgg cgatgatatt
     1261 ggccagcagg gtgaaggtga aaaacaccat cagcagcacc agcaccacaa acagtggcca
     1321 cagcacgtat tcgagaaagc tcagccagct cggcaggctg ggcataaagg tgtcgaccca
     1381 gccgccgaac tgctgcatgg ccaggtaaat caggccgata aacaggatca ggttgatcgt
     1441 caggggcagc agcacaaaca gccgcaggcc agggctcagc accagtttca ggccttcgct
     1501 gaggtattgc gggccggtca gtacgggagc aggcatgggg agagtctccg cgaggttgaa
     1561 gtcgcgccga ccttaccggc tttgtgtggc aggtgaaagc ctctgcaacg ggcgctaacg
     1621 attacctgcc ggggttatag cgcttgccta tgcagggtat tggcaaagca tatttccgcc
     1681 gtgcgctggg cgggcatagg ctgcgcggca actgatggtt tatccctttg cggggcttcc
     1741 actcttaatg ccttcccctg gtgcttgtgg acgtcccttt ttattgctgc cggcattgcc
     1801 gggaggagtt gttgatgtct gccactcgtc atgcccgctt gatcattctg ggctccggcc
     1861 ctgccggcta tagcgctgcg gtctacgccg cgcgggccaa tctcaagccg ctgctgatca
     1921 ccggcctgca ggccggtggc cagctgacca ccactaccga ggtggataac tggccgggcg
     1981 atgtacatgg cctgacgggg ccggcgctga tgcaacgtat gcaggagcat gccgagcgct
     2041 ttgagacgga aatcgtctat gaccatatca atgccgtgga cctggccggc aagctgttca
     2101 ccctggtcgg cgacagcgcc acttacagct gtgatgcatt gatcatcgcc accggtgcca
     2161 gtgcccgtta cctgggcttg cccagcgaag aaagctttat gggtaagggc gtttccgcgt
     2221 gcgccacttg cgatgggttc ttctaccgca atcgtgaggt ggcggtggtc ggtggcggca
     2281 ataccgcagt ggaagaggcg ctgtatctgg ccaatatcgc cagcaaggtg accctggtgc
     2341 atcgtcgtgg cagcttccgt gcggagaaga ttctgcagaa caaactgcag gcgcgggtcg
     2401 ccgaaggcaa gattgtgctc aagctgcacg ccgaggtgga tgaagtgctc ggcaacaaca
     2461 tgggcgtgac tggcgtgcgg gtgaagaaca acgatggcag ctttgatgag ctgagcgtcg
     2521 aaggcctgtt cgtcgccatc ggccatacgc cgaacacctc gttgttcgag gggcagctgg
     2581 cgctgaagga cggctatctg gtggtcaacg gcggccgtga cggcaatgcc accgccacca
     2641 gcgtgccggg cgtgtttgct gccggcgatg tggctgatca cgtctaccgc caagcgatca
     2701 cctcggccgg tgccggttgc atggcggcgc tggatgtgga gcgttatctc gacggtcagt
     2761 aggtctgtga gtacgaagac cttgatgtga accgcaatgc cgttcacttg gatcaagtga
     2821 gcggcatctt ttatgtggcc ggcaatatgt ggcgatggcg aaaccgcgta ggaggcgctt
     2881 tagcggcgag ctttttagtg gttgtgtcgc ggctaaagcc tctcccacat cagcggtggt
     2941 tcctcagacc caggtattca gcttcaaatc ccgcgaactc atacgcagaa actggtcgct
     3001 ggctttgacc agcaaggcgt gttcatcgac ggtcagctcg ctgctctttt tgccctgcgc
     3061 ttcgctgcgg tacagggttg ttgcaccttc ttcgcgcagg cgctggtagc gctggctgcg
     3121 tgcttccact ttgagggtca gcaggtcaac gtaggcgact tgcatcagtt cactctgccc
     3181 ggtttccaga gcacaacgct gcagggcagg ggtgtgggtg aagggcgatt cggagagcat
     3241 cacctgctgg cattcgctga ggtccgggcg tggctcaccg ttgtggaacc agttgccacg
     3301 gatatcgcgc ttgaggttga ggctgcgctg accgtggttg tcgaccttca gccacaggcg
     3361 acgaaagcgc cagcgcgggt cgcagttgag cacgtaggtg gcggcgatgc tgtggccttg
     3421 caggctttgc atcaggtggc tggtggcgtt gatgccgtgt tcatcgatgt tcaggcgcag
     3481 gttttccacg ccggggttgt gccatggttt ccataccaag gtctgctgca tctagcgact
     3541 ccaagccagg tagggttgga gaaaccgggg aagtgcggcc ataacgcagc acggagaacc
     3601 cccctggtgt tctcgttcaa gtaatggcgc gcagcatagg gggattcaag ggtgatgtct
     3661 gtcagctttt gccgactgtt gtgcaagttt ctatggcctg ccattcctgg cggccaccct
     3721 gcgggccgtc gctgcgcgac gttaaaaatg gctcccggcc attttttgcg cgttagctca
     3781 agcgagtagt tacagactgt tgcgctgcag cgatggctgc tcgaagcgca cgtccgccag
     3841 cccggtctcc atcagtgcac ggatattgcc gtggtcgctg ccgctcgggg tggccagcac
     3901 gctgcggtaa tgttcgccaa agcacagcag ggtttcttcc aaactcaggc cttcgagcag
     3961 ggccaggccg agggtcttgc acgagccttc gttctgcccg gccgtgttgg ctacattgcc
     4021 attgctgaat gcgctgggct ggtactggta gtgctcggca ataaacgcca gggtttcggc
     4081 gaaggtgaag tgctcgccgc gcagcttggc gcgaaagtcg ttgaggctgc tcattacttt
     4141 tttcctttgc tgaaggcggc ctgctgatcg gcgctggctt ccttctggta tttggctttc
     4201 cactcggcat acggcatgcc gtagacctct tcgcgcgctt catcgggggt gatggccacg
     4261 ccgatgtcgt ctgcggcggc tttgaaccac ttggacaggc agttacggca gaaaccggcg
     4321 aggttcatca tgtcgatgtt ctgcacgtcg gagcgtgagc gcagatgttg caccaggctg
     4381 cgaaaggcgg cggcttcaag ttcgaggcgt tcttgctcgg tcatggcgat gctctctaat
     4441 ggcaggcggg gaggcgttgg cggcgatgat agaagcatgc ccgcctggcg gcaacggccc
     4501 cggcgcagga cgcgccggga atctgcagag agtttctcag cagcctttct agcggtgccc
     4561 ggcgagggtg atcgataccg actcggcaaa gcgcagggcg tgcagcttgt ccacttccac
     4621 ttcggcgtag catacgtccg ggttggccat taccaggtcg agaatctcct gggtcatgcg
     4681 ctccagcaga gcgaagcggt tttcctctac atggcgaatc accgccttgg tgatggtgcg
     4741 gtagttgagg gcgtggtcga tatcgttatc gcgcaccgct tcaatggcgg ggtagaggat
     4801 ggtcaggttg atcaacacgt cctgcttgtt gttgatctcc tcttccttga tgccgatata
     4861 ggtgcgcagg cgcaggtcct tgacgcggat gcgcgccatc ccaggttcca gtcgcggcat
     4921 tacttgctcc gtccgatcag ttgcaggaat tcgtggcgag tgttgcagga ttcgcggaag
     4981 gctccaagca tcaccgaaga agtcattacc gagttctgct tctccacgcc gcgcatcatc
     5041 atgcacatgt gcttggcttc gatcaccacg gccacgccag cggcgttggt gacctgctgg
     5101 attgcgtcgg caatctgctt ggtgaggttt tcctgaatct gcaggcgccg cgcatacatg
     5161 tcgacgatgc gcgccacttt cgacaggccg agcaccttgc cggtggggat ataggccacg
     5221 tgggccttgc cgataaacgg cagcatgtgg tgctcgcaca gcgagtacag ctcgatatca
     5281 cggacgatca ccatctcgtc gttatccgac tcgaacagcg caccgttgac cacttcttcc
     5341 aggctcttgt ggtagccgtt gcacaggtac tgcatggctt tggcggcgcg cttgggcgtg
     5401 tccagcaggc cttcgcgttc ggggttctca ccgacgccga gcaggatctc gcggtagtgg
     5461 tcgggcagtt ctggggtcat tgcagggtcc tcgcggccag gctcatttga gatggcggcc
     5521 gccgttgaca gtaagggttg tgccggtgac ataggggttg tcgagcaggt agcgcaagct
     5581 ctggtagatc acttgaggcc cgggttcgat gcccagtgcc gatttattca gggttttatt
     5641 gcggtaattg gcatcgtcgt ccgggttgaa catgatcagc gccggggcaa tgccgttgac
     5701 tttgatctgc ggggcgaagc gtgcggcaaa cgacagggtc aggctgtcca gcccggcctt
     5761 gctggcgcag taggccggcc gcttggcgct gcccttgcgc gccacatcat cactgatatg
     5821 tacgatatcg gcgggcgttg aacgcgccag cagcgcctgg caatgcaggt tgatcaggta
     5881 cggcgcgagc atgtgcacgc taaacagctg ctgaaaggca gccgcttctt cgcccggctc
     5941 ttccatcagc cagtcggagg cgttgtgcac gatggcgcgc aggctgtcgg tgtggctctt
     6001 gagttgctcg ataaacgcca ggatgccggc ttcactggcg aagtccgcct gcagggctat
     6061 ggcaccgcgt tgcctgagcg catcaatgct gtcgcgctcg ctgcggtagg taacgattac
     6121 cgggtgaccg tcatccagca ggcgttcggc gcagtacagg cctatacgct ggctggctcc
     6181 ggttatcagg ataggggcgg cgctgacgct catgctcgac tcggcagaaa gaaaaaggtg
     6241 ggttcagcga cgctgcgccg ggttacctga ccagtaagac gcggcctcga ctgaaccaga
     6301 cgtaaagtta taccagcaat ggtatttgta caaccatgcg ccctgtgcgg gtactggtct
     6361 gtttcatgct ttttagccta tttcattgag ctgtatcggt attttttgca cgggcgggct
     6421 gaataaaatt gtttttatcg tgtacaagat tggttttctt gtacaggtat ctgtcgtatg
     6481 aagccgtggc agttaagcat tgcagtgctg cgtgggcacg ttaaccatgt tgaggtgcga
     6541 acgctgcgct gttgtggcta caaggtgcag gtggtcagca agactggcag cgtgcgtacg
     6601 ttggagtatg cgtcggggcg cccgctgtgg cctgggctgg cattgctcaa ggcgtgtata
     6661 cgccgctgcg gcgtgcagca gatgctgttg ctgcagtcgg aaagccacga tgaaattatc
     6721 ggtcgcccag cgttgcttga gcctgatcca ggcttgtgga tcaagctgcg ctgatgactt
     6781 attgcggttt tttgctgaca gcgcacgcag cgtttttgca gcgccggctg gcatccgggt
     6841 ggggcagcca gtcgaggtgc gggcgtagcc ggcagaacag gcggtagccc agttccagca
     6901 caggccgcaa tgccggccag gtcagtggcg tggcccagcg ctcaagaccg gctgcgcgcc
     6961 agctccacag cgtggcatcc agcccggtaa cccattggcc gtcggcgaag cgcgcatgca
     7021 ggcggttctg cagggtttct cggctgtggc cgacggactc gggatgaaag tcgttgaggc
     7081 cgatatcgac caggcacagg cgctgcacat cggcgcgctg acgcagcaac cggatctcgc
     7141 gggcgcacaa cgggcagtcg ccatcgtagt agagggtcag gggccagcag ggctgcttgt
     7201 tcatggtcta actctttgta taagtcttgt acaaagtatg ccggaaatag ctgagttaca
     7261 atgcgggcaa cattttttct gtgatacctg cgcccatgtc cgatcacgtt ggtactgcat
     7321 ccctcgtttc gaatgctctc aagcaggaag agttatttcc tattcgtgaa gtagcgcgcc
     7381 tgaccggcgt caatccggtc accctgcggg cctgggaacg gcgctatggg ctgatccagc
     7441 caacccgaac cgacagcggt catcgcctgt attcgcaggt ggatatcgag tccgtgcgca
     7501 gcatcctggc gtggatcgag cgcggtgtgg cggtgagcaa ggttggcaag attctggcca
     7561 aaagcagctc gatcaaatcc gccagttcac cggtctatga agaagtcagc gccagcgagt
     7621 ggggcgagtg gcaagcccaa ctgcgccgcg ccatcagcgc cttcgacgag ctgcgcctgg
     7681 atcgtctata cggtcagatt ttctccagct acccgctgcc ggtggtgttt caggacatcc
     7741 tgatgccgct gtggcaggag ctgttgctgc gtcaggatga atacggccag accagcgaat
     7801 ggctgttcct cgatacgttc ctgcgtgccc gtaccctgca gcgcctgcag gtgctgcgcg
     7861 cccaggttga ggagcgcgtg ctgctggcgg ctttgccggg ccattgccgc gagctggaac
     7921 tattggtcgc cgggttgctg ctgagcagcg ctgacactgc cgtcagcgtg ctagggctgg
     7981 gacaaccgtt ggaagagttg accctgatct gcgaaaaaat gcagccccag gccctggtgc
     8041 tgttttccaa ccagccgccc agcgatgacc tgcccaaggt gctctcgcgt ctggcgttgg
     8101 cgctggattg cccgctggtg atggctggcg atggtgctga cctcgccgag gaaagcctaa
     8161 gtggttcgcc gattgcctgc ctgggcaatg aagggcgtct gatgcagcgc cgtctgcagc
     8221 agtttctggc tggccatctg gatacctgat tcttctatta agcacgtctg aataagcgcg
     8281 gccgccgtta gggcagccgc gctttcgcgt gtctgcaatt tagtgtcgat ttcgattagt
     8341 tgtacagctt gtacagaaaa ttgcttgcca tatgggtctt taggctatac aaaagctata
     8401 cattatttat ttatgtacag cttgtggcca ctcgtacagc ttgaggactg agtaacatga
     8461 ctcaataccg cgcacccctt cgtgatatgc gtttcgtttt tgacgaggtg ctcgacgctt
     8521 atgccacctt gcagtcgctg cctagtcagc gcgagttcgg agacgacctc ggtggcgcca
     8581 ttctggaaga agccgccaag cttgcggaga acgtactggc cccgctaaat ggccctggtg
     8641 ataaacaggg ctgccattac gatccggaaa gtaaatcggt caaagcgccg gacggtttcc
     8701 gcgccgcgta tcagcagttc gccgaaggtg gctggaccgc tctggcctgt acgcccgagt
     8761 tcggcggcca aggcctgccc catgtgctga acatgatggt cgaggagatg gtctgctcgg
     8821 ccaacctgtc gctgggcatg tacccgggcc tgacccatgg cgcaatcaat gcgctgaccg
     8881 ctcacggcac ccgcgaattg caggagcgct acctgcccaa gctgatcagc ggcgaatgga
     8941 ccggcaccat gtgcctgacc gagccgcagt gcggcaccga tctgggcctg attcgcaccc
     9001 gcgccgtacc gcaggctgac ggcagctatg ccatcagcgg caccaagatc tggatcactg
     9061 gcggtgagca cgacttggtc gacaatattc tgcacctggt actggccaag ctgcccgatg
     9121 caccggatag cgtgaaaggt atttcgctgt ttctggtgcc caagttcctt gcggacggca
     9181 gccgtaaccc ggcgttctgc ggcggcctcg aacacaaaat gggcatcaag ggctcggcca
     9241 cctgcgtgat gaatttcgaa ggcgcccagg gctggctgat tggcgagcca aacaagggtc
     9301 tcaagtgcat gttcaccatg atgaactcgg cgcgcctgat ggtcggcatg cagggccttg
     9361 gcattgccga aagcgcctat caggtcagcc tgggcttcgc taaagagcgc ctgcaaagtc
     9421 gctcgctgtc tggcccaaaa gcggcggaca agccggccga cccgatcatc gtgcacccgg
     9481 atgtacggcg catgctgctg cgccagaagg tgatgatcga agggtgccgc gcgctggcct
     9541 atttcaccgg cctgcatgaa gacgttgccc atgaccatga agaccctgtc gtgcgcgaac
     9601 aggccgatga tctggtgcag ctgctgactc cggtggtgaa gtccttcctc accgatgagg
     9661 gttttaactg cgccaacgaa ggcttgcagg tgctgggcgg ctccggtttc accgaggact
     9721 ggggcattga acagctggtg cgtgattcgc ggattactcg catctacgaa ggcaccaatg
     9781 gcattcaggc gcttgatctg gtcgggcgca agctgggcct gggtggcggc cgcgcggtgc
     9841 gtaattactt cgccctggtc gagggctggc tcaaggccaa cgccgaggca gaacacgtcg
     9901 ctgcagtggg tgctgcgctt aagcaattgc agcaggccac attgtggatt gccagtgaag
     9961 ggatgaaaga ccccgagcag gccggtgcgg cggcaacccc ttatctgcga ctgtttgccc
    10021 tgaccagcct ggcctggatg tgggcacgta tggcggcggt ggccaaggcc aagctggcgg
    10081 ccggcagtac ggaaaccctg ttctacggcg ccaagctgaa gtctgcggat ttctttatgg
    10141 cgcgcatcct gactgaaacc gacagcctgc tggctgaggt caaggccggc aaagccacgc
    10201 tgatggcctt cagtgaagag gaatttgctg cctgaaacga ctgcgcaatc cggtagccat
    10261 cagttccgcc cattttcggc tatcggattt ttttatccct gctgcaggag cttctcctgc
    10321 actttaggcc cgcgctgatt gcgggtcttt ttttgtccgg gatttactcg gcatatgtcg
    10381 gtgcctcagc ggcgcccggc ctgcagccgt tgttcctgct gcaggtgttg caggtagata
    10441 aactggcgca gctgctcatc gctgtgcggg tccagggcat gcagttggta ggccagcagg
    10501 ccgtcctcgg tttcgcgaac gaacaccccg gccacggcaa tcggcgtgag ctcgtccacc
    10561 ggcaatatca gctgaaagcg cttgggggct ttgcgtcgtg cccgatgttc gatcagcgcg
    10621 ccattcatcg acagctgatg gatccacagg tcgctcggtt gcgcacttgg cgtgtgcagc
    10681 ggcaacggct cggccagtgg cgagcgccag gcacggttga cttcaccctc ctcgaaaatc
    10741 tgcggggcgc tgagctgcaa gcagggggtg ttctgttcat cccggctaag gtgcaggtca
    10801 aaggtcaggt gctgattggc gatatgcgca tgcaggctta gctgctcatc gacggcgcaa
    10861 tgcgagagca gctcgctgag ctgtttgcca atatccactg acaagtgcgg ttggctgcgc
    10921 tgctcggtgg ttgagggctg atgcagctgg cggataaacg ctaactctgc ctgggtcagg
    10981 agtgcctgtt cttgcatgga agcggtctat atcaagctct ggagctgttg tgaccgctgc
    11041 tttgggaatt agttactgcc ggtcgtcggc tgtaaacgcg ccagttcggc cttgaggctg
    11101 gccaattcgg cttccagttg cttgacccgc tcttgggctt cgacttgctc ggtgacgttc
    11161 ttctggatgc cgatgtagta ggtcagctgg tcggcatcgt tgaccaccgg ggtaatcgac
    11221 agctcgttcc agaacggcgt gccgtccttg cggtagttgc ggatgatctg ccggcagggg
    11281 cggtgcgcct tgaccgcttc gcggatggcg gccaggcccg gttggtcacg gtcattggcc
    11341 tggaggaagc ggcagtcctg atagaggatg tcttcgctgg cgtagccggt gagcttttca
    11401 aaggcggggt tggcatagat caggatgttg tcgtcaccct cctgttcggc caccacgatg
    11461 ccgtcgttgg aggcgttgat caccagttgc agcagtttgg cgttgatcat gaggcgattt
    11521 cccaggctat gtcggctgca ttctaaaaca tccgggcggg ctgtctactt gcggcataat
    11581 gcccggctta ttgatttggt tctgactgtt tcggagtttt gcatgaagat cgccatcctt
    11641 tccggctcgg tgtacggcac ggccgaggaa gttgcccgcc acgccgagcg gctgctcaag
    11701 gccgccggcc atgaggcctg gcataacccg cgcgccagcc tggcggatat tcaggccttt
    11761 gcgcccgagg cttttctggc ggtgacctca accaccggca tgggcgaatt gccggataac
    11821 ctgcagccgt tgtactacgc cattcgcgat catctgccgg cctggaacgg cctgccgggt
    11881 ggggtgattg ccctgggtga ttccagttat ggcgatacct tctgcggcgg tggcgagctg
    11941 gtgcgcgagt tgtatgccga gctgggcatc cgtgaggtgc agccgatgct gcgcctggac
    12001 agcagcgaaa gcgtgacccc ggaaagcgac gccgagccct ggctggcgga ctttattacg
    12061 gcgctgcaag gctgatacag cccgtacgca tgcattgagc gtggcgggtt gtcgttaata
    12121 tcccgaactg gtttgactcg caaggccacc acgctggccg ccagagcgac tcacttttgc
    12181 ggcatccctc gctagactcc ttggcacttc aataacaaaa gccgaggaat ccatcatgac
    12241 cgttgcccat gcattgcctg ttgtcagcct caaggatcag gtgtctgctg ccgaatggca
    12301 gactcgcgtg gacctggccg cctgttaccg gctgatcgcc ctgtatggct gggatgacct
    12361 gatcttcacc catatctcgg cgaagattcc cggcagcgaa gatttcctga tcaatcccta
    12421 cggcctgatg ttccacgaga tctccgcctc cagcctgctg aaggtcgatc tggccggcaa
    12481 caagctgatg gacagcccct tcgacatcaa cccggccggc tacaccatcc acagcgccgt
    12541 gcatgaagtg cgtcacgacg tcggttgcgt gctgcatatc cacaccccgc aggggattgc
    12601 cgtatccgcg cagaagcagg gtttgctgcc actgtcgcag cagtcgctgt ttgttctcgc
    12661 cagcctggcc taccacggct atgaaggcgt ggcgctgaac catgatgaga agtcgcggtt
    12721 gcaggcggat ctgggcgaca agaactttat gatcctgccc aatcacggcc tgctcaccgc
    12781 cgctggcagc atcgccgacg ccttcctgat gatgtttatt ctgcagcgcg cctgtgagat
    12841 tcaggtgatg gcgcaaagcg gcggcgctga gctgatccat atcccgcagc aggttctcga
    12901 cggcgcccgg gcgatgatcg ccggggtgat gaaaagcccg cagggcatgg gcggcgcctt
    12961 gccgtggccg gcgctgctgc gcaagcttga tcagcagatg cccggttacg ccgcatgagt
    13021 gcgctgccag gcatcgccct ggacgcctgg cgcgctcagg gtcgcgactt cagctttaac
    13081 ggccagccca ttcgctactg gacggcgggc gaccccgctg ccgagccgtt gctgctgatc
    13141 cacggctttc ctaccgccag ctgggactgg cattacctgt gggcgccact ggccgcgcgt
    13201 taccggctga ttgcctgcga tatgctcggt ttcggtgact cggccaagcc acgcggccat
    13261 gattacagcc tgctagagca ggctgattta cagcaggcgc tgttggcgca tcttggcgag
    13321 tcgcgcccgc tgcatgtatt ggcccatgac tacggcgaca gcgtggcgca ggagctgctc
    13381 gcccgccatc aggacgggcg catcgcgctg gccagttgtg tgtttctcaa cggcggcctg
    13441 tttccggaaa cccatcatcc ggtgctggtg cagaagctgc tgctcagccc cattggtccg
    13501 ttgatcggcc gattgttcag ccgcaagaag ctggcagcca gctttgccca ggtattcggc
    13561 ccgcagaccc agcccagcgc ccgcgagctg gatgacttct ggagtctgat tgccgccaat
    13621 aatggtcccg cggtgatgca tcgacttatc cgctatatcc tcgaccgccg cgtcaaccgc
    13681 gatcgttggg tggcggcgat gcagagcact gccgtgacga tgcgggtgat cgacggcgct
    13741 gtcgacccga tttccggggc gcatatggtg gcgcgttatc gcgagctgat ccctcagccg
    13801 gataccgtgc tgctggagca gatcggccat tacccgcaga ccgaggcacc ggatcaggtg
    13861 ctcgctcatt atttgcagtt ccgtgaacgg ctggaggccg caatatgcag cgacgcactg
    13921 tcttaaaggg tgccgccttg ggcggtgtgg cagttctggg tgccgggttc tgggcattgc
    13981 ccagcggcga ggcaccggcg gccctgagtc tggacggcgc gcagcaggtg ctggccgggc
    14041 tggtcgataa accgctgtcg agcctcaagg gctggagccc gaccgaggtg ttcaaccact
    14101 gcgcgcagag catcgagtac tccatcagcg gttaccccga actcaagccc ggctggttcc
    14161 gcagtagcgt cggcctgctg gcctttagcg tatttgccgc gcgcggggcc atgcgtcatc
    14221 cgctggatga ggccatcccc ggcgcggcac cgctaaatac gccagccacc caggcccagg
    14281 cattgcagcg tttgcagcag gcctttgccg atttcgctgc gtatcagggc gagttgcagc
    14341 cgcatttcgc ctatggcgcg ctcagccatg ccgactatgc ccaggcccat gtgctgcatc
    14401 tctataacca cctcagcctt atccgcccgg cctgagtgcc ttgccaggga aagaggcacg
    14461 ggccactaat caaaaacatc gaaccctcag agcccgcgta cggtcggttt ttatgtacgc
    14521 actcacatag gagatggaac gatgaatgac aaaaccaaac tgtgcgcaat gatcttcagc
    14581 ggcctcttgg cattttccgc tttgcctgct gtggcgggtg g
//
LOCUS       DASRUM010000140         6987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_581917, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000140 DASRUM010000000
VERSION     DASRUM010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581917"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1047)
                     /locus_tag="VFF22_09305"
     CDS             complement(55..1047)
                     /locus_tag="VFF22_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HZX17043.1"
                     /translation="MSIVISNVSKNFNAFKALNDINLNIQSGELVALLGPSGCGKTTL
                     LRIIAGLETPDSGSIAFHGEDVSNHDVRDRNVGFVFQHYALFRHMTVFDNVAFGLRMK
                     PKGERPNENMIKKKVHDLLDLVQLDWLADRYPEQLSGGQRQRIALARALAVEPKVLLL
                     DEPFGALDAKVRKELRRWLARLHEEVHLTSVFVTHDQEEAMEVADRIVVMNKGVVEQI
                     GSPGEVYENPASDFVYHFLGDANRLYVGDDHHVLFRPHEIHLSRQAEEGHQAGEVRDI
                     RPLGAITRVTLKVAGQAEPIEAEVAKDHLSLANLTRGETLYFKPKAGQPVIQQR"
     gene            complement(1051..1923)
                     /gene="cysW"
                     /locus_tag="VFF22_09310"
     CDS             complement(1051..1923)
                     /gene="cysW"
                     /locus_tag="VFF22_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HZX17044.1"
                     /translation="MSSVTLSASASANAARRGNVLGRRLLIISAWLVFVLFLVLPLFI
                     VLSEALKHGFGTFFGAIFEADAISALKLTLLAVGISVPLNLVFGVAAAWCVSKYEFRG
                     KSLLVTLIDLPFSVSPVIAGLIYVLLFGSQGFFGPWLREHDIQIIFALPGIVLATVFV
                     TVPFVARELIPLMQEQGTQEEEAARLLGANGWQMFWHVTLPNIKWGLIYGVVLCTARA
                     MGEFGAVSVVSGHIRGYTNTLPLHVEILYNEYNHVAAFSVASLLLLMAVFILLLKQWS
                     ESRINRLKASAGEE"
     gene            complement(1934..2752)
                     /gene="cysT"
                     /locus_tag="VFF22_09315"
     CDS             complement(1934..2752)
                     /gene="cysT"
                     /locus_tag="VFF22_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HZX17045.1"
                     /translation="MSRRTSPVIPGFGLTLGYTLVYLSLLVLIPLGAMFVHAAQLTWA
                     EFWAIISAPRVLAALKLSFATAFAAAVINGVIGTLLAWTLVRYTFPGRKVIEAMIDLP
                     FALPTAVAGIALTALYAPSGLVGQWTTDLGFKIAYTPIGITLALTFVTLPFVVRTIQP
                     VLADIPREVEEAAACLGVKPLQVFRHVLLPALLPAWLTGFSLAFARGVGEYGSVIFIA
                     GNMPMKTEILPLLIMVKLDQYDYTGATAIGVLMLVVSFILLLLINLLQRQIAKS"
     gene            complement(2963..3973)
                     /locus_tag="VFF22_09320"
     CDS             complement(2963..3973)
                     /locus_tag="VFF22_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZX17046.1"
                     /translation="MSISRFAFAALASALTIAGPAGPVAAAETLLNVSYDPTRELYSE
                     FNAAFNKHWQAQGNEAITIQQSHGGSGKQARAVIDGLRADVVTLALAGDIDELYKLGK
                     LIPEKWQERLPQSSTPYTSTIVFLVRKGNPEGIKDWDDLVKPGVEVITPNPKTSGGAR
                     WNFLAAWAYAQQKYGSEAKAQAFVEKLYKNVPVLDTGARGSTITFVNNQIGDVLLAWE
                     NEAFLALKEEGGDNFEIVAPSLSILAEPPVAVVDENVDKKGTRKVAEAYLNYLYSDEG
                     QRLAAKHFYRPRNQAIAGEFAKQFPELKLVTIDKDFNGWKTAQPKFFNDGGVFDQIYQ
                     AQ"
     gene            complement(4138..4320)
                     /gene="oscA"
                     /locus_tag="VFF22_09325"
     CDS             complement(4138..4320)
                     /gene="oscA"
                     /locus_tag="VFF22_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur starvation response protein OscA"
                     /protein_id="HZX17047.1"
                     /translation="MTATLQNLEDQYDASILREIQAALNGLKFGSVEITVHNGQVVQI
                     ERKEKFRPHAQSNKSS"
     gene            4581..5162
                     /locus_tag="VFF22_09330"
     CDS             4581..5162
                     /locus_tag="VFF22_09330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003211820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX17048.1"
                     /translation="MRNQTIRYLIVPGWQGSADDHWQSHWQRSLPNSARVEQADWLNP
                     RREDWVAELQRSIASDPRPTILIAHSLGCITVAHWAAQAPFESLQRVRAALLVAPADV
                     ERANCPSVLQNFAPIPQHSLPFPSLLLGSDNDTAATAARAIELARDWGSDAVILSGVG
                     HINVASGHQRWEQGFAYLYRLQSLIERYARLTA"
     gene            complement(5176..6093)
                     /locus_tag="VFF22_09335"
     CDS             complement(5176..6093)
                     /locus_tag="VFF22_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX17049.1"
                     /translation="MFKNIEGVSLDVLRVFESAARQLSFTAAASELGTSQPAISQQIK
                     RLEQQLATRLFDRVYRGIVLTDAGELLLRYVQDGLQSLDAGLLAVTAQQQHEVLQVAT
                     DFAFAAYWLMPRLQRFHQLHPEVDVSLITSERDQGALPAEIDVAINFGDGRFKHAEAQ
                     LLFREEVFPVCSPQLLKGQTLPLPPAALASLPLLHLRPENRSRWFDWAGLFRAFGLQQ
                     TPSPGSLRFDNYTLLIQAAIAGQGVAIGWRHLVDELLTQNLLCRVGDEVAHSDMGYYL
                     VLPERKRRQRLTQVFVDWLQAELAKGPGV"
     gene            6205..6987
                     /gene="betC"
                     /locus_tag="VFF22_09340"
                     /pseudo
     CDS             6205..6987
                     /gene="betC"
                     /locus_tag="VFF22_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248721.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-sulfatase"
BASE COUNT         1451 a         2126 c         2090 g         1320 t
ORIGIN      
        1 cgggccatgc ccgcgaagca gtatcgcggg catgggactg ggcgccaccc gctcctaccg
       61 ctgctgtatc accggctggc ccgccttggg tttgaaatac agcgtttcac cgcgagtgag
      121 gttggccagg ctgaggtgat ccttggccac ttcggcttcg atcggctcag cttgacctgc
      181 gaccttgagg gtcacgcgag tgatcgcgcc aagcgggcgg atgtcgcgta cttcaccggc
      241 ctgatggccc tcttcggcct ggcgcgaaag atgtatctcg tgcgggcgga acagcacatg
      301 gtggtcgtca ccgacataca ggcggttggc atcgccgagg aagtgataga cgaaatcact
      361 ggccggattc tcgtacacct cgcccggcga gccgatctgc tcgaccacgc ccttgttcat
      421 caccacgatg cgatcagcca cttccatggc ttcttcctgg tcgtgggtga cgaacaccga
      481 ggtcaggtgc acttcttcat gtaagcgcgc cagccagcgg cgcagctctt tgcgcacctt
      541 ggcatccagg gcgccgaagg gttcgtcgag cagcagcact ttcggctcca ccgccagggc
      601 gcgagccagg gcgatacgct gacgctggcc gccggaaagc tgctcggggt agcgatccgc
      661 cagccagtcg agctgcacca gatcgagcag atcgtgaacc tttttcttga tcatattctc
      721 gttcggccgc tcgcccttgg gcttcatgcg caggccgaag gcgacgttgt cgaacaccgt
      781 catatgacgg aacaacgcat agtgctgaaa cacgaagccg acgttacggt cgcgcacgtc
      841 gtggttggag acatcttcgc cgtggaacgc gatgctgccg ctgtccgggg tttccaggcc
      901 ggcgatgatg cgcagcaagg tggtcttgcc gcagccggac gggccgagca aggcgaccag
      961 ctcgccgctc tggatattca ggttgatgtc gttcagcgct ttgaacgcat tgaagttctt
     1021 gctgacattg gaaattacga tggacatgac ttattcctcg ccagcactgg ctttcaggcg
     1081 gttgatacgg gactcgctcc actgcttgag cagcaggata aacaccgcca tcagcaacag
     1141 cagactggcg acgctaaagg cggcgacgtg attgtattcg ttgtagagaa tctcgacatg
     1201 cagtggcagc gtattggtgt aaccacggat atgcccggag accaccgaca ccgcgccgaa
     1261 ctcgcccatg gcccgcgcgg tacagagcac cacgccgtag atcaggcccc atttgatatt
     1321 cggtaaggtt acgtgccaga acatctgcca accattggcg ccaagcaggc gcgcggcctc
     1381 ctcttcctgg gtgccctgct cctgcatcag cgggatcagc tcgcgggcga caaagggcac
     1441 ggtgacaaac acggtggcta agacgatgcc cggcaaggcg aagatgatct ggatatcgtg
     1501 ctcacgcagc cacgggccga aaaagccctg ggaaccgaac agcagcacat agatcagacc
     1561 agcgattacc ggcgacaccg agaacggcag atcaatcagg gtcaccagca ggctcttgcc
     1621 gcgaaactcg tacttgctta cgcaccaggc cgcggccacg ccgaacacca ggttgagcgg
     1681 caccgaaatg cctacggcca gcaacgtgag cttcagcgca gagatggcat cggcttcgaa
     1741 gatcgcacca aagaaggtgc caaagccgtg ctttaacgcc tcactcagca cgataaacag
     1801 cggcaacacg aggaacagca caaacaccag ccaggcgctg atgatcagca gcctgcgacc
     1861 gagcacattg ccgcgtcgtg cggcgttggc cgaggctgac gcgcttaggg ttacagaaga
     1921 catggcgacc tcctcaggac ttggcaatct ggcgctgcag caggttgatc agcagcagca
     1981 aaatgaagga gaccaccagc atcaacacgc caatcgccgt ggcaccggtg tagtcgtact
     2041 ggtcgagctt gaccatgatc agcagcggca gaatctcggt cttcatcggc atgttgccag
     2101 cgataaagat caccgaaccg tactcaccca cgccgcgagc aaaggccagc gaaaatccgg
     2161 tcagccaagc tggcagcaac gccggcagca gcacatggcg aaacacctgc agcggcttga
     2221 caccgaggca ggccgcagcc tcttcgacct cgcggggaat atcagccagc accggctgga
     2281 tggtgcgcac cacaaacggc agggtgacaa aagtcagtgc cagggtgatg ccgatgggcg
     2341 tgtaggcaat cttgaaaccc aggtcagtag tccactggcc aaccaggccg ctcggggcat
     2401 ataaggcggt caaggcaata ccggcaacgg cggtgggcaa ggcaaaaggc aggtcgatca
     2461 tcgcctctat taccttgcgg cctgggaagg tgtaacgcac cagcgtccag gccagaagcg
     2521 tgccgataac gccgttgatc acggcagcgg cgaaggcggt ggcaaagctc agtttgagcg
     2581 cagccagcac ccgcggtgcg gaaataatcg cccagaattc agcccaggtg agctgagcgg
     2641 cgtgaacgaa catggcaccc agaggaatca gcaccaacag gctgaggtac accagggtgt
     2701 agcccagcgt cagcccgaag ccgggtatga cgggggaagt acgacgagac atggtttggc
     2761 cttgtgcttg ttatgcgacg agctaatgac gcgttgcgag cgaaggtcag acaaggcgca
     2821 acgaccgacg ggagtaacag ccagaggctg gcccgtaggg tgagcgcctg cgaataaagc
     2881 cggcgaggga cgcggagttt acgggctgta aatgagcagt cccgagccgg ctttcaacgc
     2941 agtatgaccg agcgcagtag cgtcactgcg cctgatagat ctgatcgaat accccgccat
     3001 cattgaagaa cttcggctgt gcggttttcc aaccgttgaa gtccttgtcg atggtcacca
     3061 gcttcagttc cgggaactgc ttggcgaact caccggcaat ggcctggtta cgcggacggt
     3121 agaaatgctt ggccgccaga cgctgacctt cgtcgctgta caggtagttg aggtaagcct
     3181 cagccacttt gcgggtgccc ttcttgtcga cgttttcatc gacgacagcc accggcggct
     3241 cagccaggat cgacagcgac ggcgcgacga tttcgaagtt gtcgccgccc tcttctttca
     3301 atgccaggaa ggcttcgttt tcccaggcca gcagcacgtc gccgatctgg ttgttaacga
     3361 aggtgatggt cgaaccacgg gcaccggtgt cgagtaccgg cacgttcttg tagagcttct
     3421 cgacgaaggc ttgggctttc gcttcgctgc cgtatttctg ctgggcgtag gcccaggcgg
     3481 cgaggaagtt ccagcgtgca ccgccggagg ttttcggatt cggggtgatg acttccacgc
     3541 ccggtttgac caggtcatcc cagtccttga tgccttccgg gttacccttg cgcaccagga
     3601 acacgatggt cgaggtgtac ggcgtgctcg attgcggcag acgctcctgc catttttccg
     3661 ggatcagctt gccgagcttg tacagctcgt cgatgtcacc ggccagggcc agggtcacca
     3721 cgtcagcacg caggccgtcg attaccgcac gggcctgctt gcccgagcca ccgtgggact
     3781 gctggatggt gatggcttca ttgccctggg cttgccagtg cttgttgaac gcagcgttga
     3841 attcactgta cagctcgcgg gtcgggtcgt atgacacgtt gagcagggtt tcagcggcgg
     3901 ccaccgggcc tgcgggcccg gcaatagtca gtgcgctggc gagggcggca aaggcaaaac
     3961 ggctaatgga catggtgcag ctcctactgg gacaaatacg gggttggcgt tcaatgggtc
     4021 gatgctctcg ccacctaccg tggctatccg gcgtggtcgc gcgggctcga ctcttttcat
     4081 tgcacgccct ccggtggtcc ggtcgggctt tgcggcgtgt ggcgttcggg gtgtcagtta
     4141 gctgctcttg ttgctctgag cgtgtgggcg aaacttctct ttgcgttcga tctgcacgac
     4201 ctggccgttg tgcacggtga tttccaccga gccgaacttc aggccattca gcgcagcctg
     4261 gatctcacgc aggatgctgg catcgtactg atcttcaagg ttctgcaggg ttgcggtcat
     4321 gctgatgctc catgaaatag cccctataag cggcgaaaaa cagccgtagt cagtggtcgt
     4381 ggagcggata ctagagaggc tcttatattc ttaaaaatat tatttaagaa catttatata
     4441 ttttttaaga atataaagaa tcgcaccctc cgcgcaccga aaaatagaat ttcaggcata
     4501 agcaaataat tttttattct ttttgatatt caaaagccct ccctacactg cagccattca
     4561 catccgcagg agggcgcacg atgcgcaacc aaactatccg ttacctgata gtgccaggct
     4621 ggcaaggatc ggcagacgac cattggcaga gccactggca gcgcagcctg ccgaacagtg
     4681 cacgggtgga acaggccgac tggctaaacc ccaggcgtga agactgggtc gccgaactgc
     4741 agcgcagcat cgccagcgat ccgcgcccga ccatcctgat tgcccatagc ctgggctgca
     4801 tcaccgtggc gcattgggcg gcgcaggcac cgttcgaatc gctacagcgg gtacgtgctg
     4861 cgctgctggt cgccccagcc gatgtcgaac gggccaactg cccgtcagtg ctgcagaact
     4921 ttgcgccgat cccgcaacac tcactaccct tccccagcct gttgctcggc tcggataacg
     4981 acaccgccgc cactgctgcg cgagcgattg aactggcccg cgactggggc agcgatgctg
     5041 tgattctcag cggcgtcggg catatcaatg tggcgtccgg gcatcagcgc tgggaacagg
     5101 gctttgctta cctgtatcgc ctgcagagcc ttatagagcg atacgcgcgc cttactgcct
     5161 gatggccacg gctggttata cccccggtcc cttggccagt tcggcctgca gccaatcgac
     5221 aaacacctga gtcaggcgtt ggcgacgttt gcgctcgggc agcactaggt agtaacccat
     5281 atccgaatgc gcgacttcat cgcccacgcg gcacagcagg ttctgcgtca gtagttcatc
     5341 aaccagatgc cgccaaccga tggccacgcc ctgcccggcg atggccgcct gaatcagcag
     5401 cgtgtagtta tcgaaacgca ggctgcctgg gctgggggtt tgctgcaggc cgaaggcgcg
     5461 aaacagcccc gcccaatcga accagcgcga acggttttcc gggcgcaggt gcagcagcgg
     5521 caagctggcc agcgcagccg gtggcaaagg caacgtttgc cccttgagca gttgcgggct
     5581 gcacaccggg aacacctctt cgcgaaatag cagctgcgcc tcggcgtgct tgaagcggcc
     5641 atcgccgaag ttgatcgcca catcgatctc cgccggcagc gcgccctgat cacgctcgct
     5701 ggtaatcagg ctgacatcca cctccggatg caactgatga aaacgctgca agcgcggcat
     5761 caaccagtaa gcggcaaagg caaagtcggt ggccacctgc agcacctcat gctgctgttg
     5821 cgcggtgacc gccaacagac cggcatccag gctctgcaga ccgtcctgca cgtagcgcag
     5881 caacaactcg ccggcatcgg tgaggacgat gccgcggtaa acccgatcaa acagccgcgt
     5941 ggccagctgc tgctccaggc gtttgatctg ctggctgatc gccggctggc tggtgcccag
     6001 ctcactggct gcggcggtaa agctcagctg gcgggcagcg gactcgaaca cccgcaacac
     6061 atccagcgac acgccttcga tgttcttaaa cataagctca ggttatccaa tgcatctact
     6121 gacagcaggt ttacccggct tttgcttagc cggatgatca agccagcact gtcgcataaa
     6181 tcttttatat ggaatacggc tgccatgaaa cgcccgaaca tcctctttat catggccgat
     6241 cagatggccg cgccgattct gccgctgcac gatgccgcct cgccgatcca gatgcccaac
     6301 ctgatgcgcc tggccgctga ggccgtggtg ttcgatgccg cctactgcaa cagcccgctc
     6361 tgcgcgccct cgcgttttac cttggtcagc ggccagttgc cctcgaagat cggcgcttac
     6421 gacaacgccg ccgacttccc cgccgatgtg ccgacctacg cccattacct gcgccgcctg
     6481 ggctaccgca ccgcgctgtc aggcaagatg catttctgcg gcccggacca gctgcatggt
     6541 tatgaagagc gtctgaccag cgatatttac cccgccgact acggctgggc ggtgaactgg
     6601 gatgagccgg acgtgcgcgc cagctggtac cacaacatgt cctcggtgtt gcaggccggg
     6661 ccatgcgtgc gcaccaacca gctggatttc gatgaagagg tggtgttcaa ggcccagcag
     6721 tatctgtatg accacgtacg cggcgacggc gagcagccct tctgcctgac cgtgtcgatg
     6781 acccaaccgc atgaccccta caccatcccg agcgagttct gggacctcta ccgcgatgag
     6841 gacatcccgc tgccgcgcca gcatattgat caggccgagc aggacccgca ctcgcaacgc
     6901 ctgatgaaag tcatcgacct tgggacaagc cgctgcccga acagaagatc aaggacgcgc
     6961 gccgcgccta cttcggtgcc tgcagct
//
LOCUS       DASRUM010000141         5031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1419525, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000141 DASRUM010000000
VERSION     DASRUM010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419525"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VFF22_09345"
     CDS             <1..601
                     /locus_tag="VFF22_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17050.1"
                     /translation="AVLPHKHPHELLDSVVKERVVDPFVSTEARILQQPHLPSSYFSK
                     FFPKKFFLLNHLRFRSTCSFSSAGGEFYSVSNRCQPPLLPLSIISTEPLQTQTHHPEE
                     EAHSTQLSLLCNPLFKYNFLFYKKFKEPQTPEEVRIIGTTKRRSIDCYAFVSTSTKPR
                     AVATQSNNASPTETTKPLKLTLQRLCRNNHASVQSITRP"
     gene            complement(588..1787)
                     /gene="tyrS"
                     /locus_tag="VFF22_09350"
     CDS             complement(588..1787)
                     /gene="tyrS"
                     /locus_tag="VFF22_09350"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZX17051.1"
                     /translation="MKSVEEQLALIKRGAEEVLVEAELVEKLKRGQPLRIKAGFDPTA
                     PDLHLGHTVLINKLRQFQELGHQVIFLIGDFTGMIGDPSGKSATRPPLTREQVLENAE
                     TYKTQVFKILDPAKTEVAFNSTWMDKMGPADFIRLTSQYTVARMLERDDFSKRYSTNQ
                     PIAIHEFLYPLVQGYDSVALQADVELGGTDQKFNLLMGRELQRSYGQSSQCIVTMPLL
                     EGLDGVKKMSKSLGNYIGIQEAPGVMYNKLVSMPDALMWRYFELLSFRSMDEIEQFKR
                     DVEQVANPRDIKIKLAEEIVARFHGEEAALNAHRSAGNRMKEGELPEDLPEIEVLSAE
                     DMPIAALLNKAGLVKNAAMARDLLGSGSVRVDGEVVDRAFIYKVGAVHVCQAGKKAFA
                     RITLKAE"
     gene            2000..3418
                     /locus_tag="VFF22_09355"
     CDS             2000..3418
                     /locus_tag="VFF22_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZX17052.1"
                     /translation="MTSPTKAPPLYPKSHILAASGVAALLSLTLLVFPTREVEAQKTY
                     INLELDNGSEYAELQDEQYLDASQTLNKSQSPFANTAATEQPAGDELKAAQAEEPQEE
                     IDPLKHTIRVENGDTLSTVFAKVGLSATTLHDVLNSSKEAKQLTRLKVGQALEFNLNT
                     DGQLESLHSKLSDLESIALTKSEKGFSFKRELVKPEVQTTYSHGVINSSLFLSAKRAG
                     LSHSLTMDLANVFGYDIDFAMDIREGDEFELIYEKKVVNGKQVGTGNILSARFTNRGK
                     TYTAVRYTNKQGNSSYYSATGESMRKAFIRTPVDFARISSRFSTGRRHPVLNKIRAHK
                     GVDYAAPRGTPIKAAGDGRITLAGRHGGYGNAVIIQHGQRYRTLYAHMNGFAKGIRSG
                     GNVKQGQIIGYIGTTGLSTGPHLHYEFQVNGVHVDPLSQKLPMADPIASSEKARFMQL
                     SKPLMAQMDKEKSTMLALNNQR"
     gene            3422..4513
                     /locus_tag="VFF22_09360"
     CDS             3422..4513
                     /locus_tag="VFF22_09360"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011063788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HZX17053.1"
                     /translation="MPLYLGVMSGTSLDGLDIALIEQEHSIRLLGTRYLPMPDELRSA
                     LLSLCASGPDELARAAIAEQQWVALAAQGISELLIQHDIPAHSVRAIGSHGQTVRHEP
                     ARGFSIQIGNSALLAELTGISVVSDFRRRDVAAGGQGAPLVPAFHQALFASEGSTRAI
                     LNIGGFSNLSLLEPERPVHGFDCGPGNVLLDAWIQRHQGLTYDQDGQWAASGQIQSQL
                     LQALLSDPYFQTQGPKSTGRELFNLDWLDQHLQNLPAFAAADVQATLLELTAISITEA
                     LKAAQQNTEMLLVCGGGAHNHTLMRRLAQLLPNSKVSSTDAHGVPADWVEAMAFAWLA
                     HCCLEGIPANRPSVTGARGLRVLGAIYPA"
     gene            complement(4590..4940)
                     /gene="erpA"
                     /locus_tag="VFF22_09365"
     CDS             complement(4590..4940)
                     /gene="erpA"
                     /locus_tag="VFF22_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HZX17054.1"
                     /translation="MSVETFTPSAMQFTQGAANKVKSLVDEEGNPRLKLRVFVTGGGC
                     SGFQYGFTFDEEVADDDTIIEREGVSLVVDPMSFQYLAGAEVDYQEGLEGSRFVINNP
                     NATTTCGCGSSFSI"
BASE COUNT         1170 a         1581 c         1269 g         1011 t
ORIGIN      
        1 agcagtctta cctcacaagc acccacacga attgcttgat tcagttgtta aagagcgggt
       61 ggttgatcct ttcgtctcaa ccgaggcgcg cattctacag cagcctcatc ttccgtcaag
      121 ctatttttcg aagttctttc ccaagaagtt ctttctactc aaccacttgc gctttcgatc
      181 aacatgtagc ttctcgtcag cgggaggcga attctacagc gtttctaacc gctgtcaacc
      241 gcctctttta ccgctttcga tcatctcgac cgaacccctt cagacccaaa cacatcaccc
      301 tgaagaggag gcgcattcta cgcagctttc gctgctttgc aacccccttt ttaaatataa
      361 cttcttgttt tataagaagt ttaaggagcc gcaaacgcct gaagaggtgc gcattatagg
      421 gactacaaaa cgaaggtcaa tcgattgtta tgcttttgtt tcaacatcca cgaaaccaag
      481 ggctgtagcc acacaatcca acaacgcgtc gcctacagaa acgacaaagc cgctgaagct
      541 gacgcttcaa cggctttgtc gtaacaatca cgcaagcgtg caatcgatta ctcggcctta
      601 agcgtgatac gcgcgaacgc tttcttgccg gcctggcaga cgtgcacagc acccacctta
      661 tatatgaagg cgcgatccac cacctcgcca tccacacgca cactgccgga gcccaacaga
      721 tcacgcgcca tcgcagcgtt cttcactagg cctgctttat taaggagcgc ggcaatcggc
      781 atatcctcag cagacagcac ctcgatctct ggcagatcct cgggcagctc gccttccttc
      841 atacgattac ccgccgagcg atgggcattg agcgccgcct cttcgccatg gaagcgggca
      901 acaatctctt cggccagctt gatcttgatg tcacgcgggt tagcgacctg ctcgacatca
      961 cgcttgaact gctcgatctc atccatcgaa cggaagctga gcagctcgaa gtagcgccac
     1021 atcagcgcat cgggcatgga taccagcttg ttgtacatga cacccggcgc ttcctggata
     1081 ccaatgtagt tgcccagtga cttggacatc ttcttcacgc catccaggcc ttcaagcaac
     1141 ggcatggtga cgatgcactg cgaggactga ccataggagc gctgcaactc gcgtcccatc
     1201 agcaggttga acttctgatc ggtgccgccc aattcgacat ccgcctgcaa cgctaccgag
     1261 tcataaccct gaaccagcgg gtagagaaac tcgtgaatgg cgataggctg attggtcgaa
     1321 tagcgcttgc tgaagtcatc gcgctccagc atccgcgcca cggtgtactg cgaggtcagc
     1381 cgaatgaaat cagctggtcc cattttgtcc atccaggtgg agttgaaggc gacctcggtt
     1441 ttcgccggat cgagaatctt gaacacctga gttttatagg tctccgcgtt ctccaatacc
     1501 tgctcgcggg tcagcggtgg gcgcgtggcg ctcttaccgc tggggtcgcc gatcatcccc
     1561 gtgaagtcgc cgatcaggaa aatcacctga tgccccaact cctggaactg tcgcagctta
     1621 ttaataagca cggtgtgccc gaggtgcaga tccggcgcag tcggatcaaa acctgctttg
     1681 atacgcagtg gctgaccacg cttgagcttc tcaaccagct cagcctcaac cagaacctct
     1741 tctgcgccac gcttgatcag cgccaactgc tcttcaaccg acttcatgac aggactcacg
     1801 accactcaaa acaaaaggga accaaccata caagatcgca gacaaatcac aagttctgcc
     1861 cgccacccac ctacgctaag accacctcgc gacaaccggg gttgcctagc tagcgatttg
     1921 gttatatttt atacagttac tgcatattca tcatttcgtt tatctatttc ttttcatttt
     1981 taaagtcaaa aagcagccta tgacctctcc aactaaagcg ccgcccctgt acccgaaaag
     2041 ccatattctg gcggccagtg gcgtcgccgc cctactgagc ctaaccctac tagtattccc
     2101 cacccgcgaa gttgaagccc aaaagaccta catcaacctt gagctagaca acggctctga
     2161 gtacgccgag ctgcaagatg agcagtactt agatgcctcg cagacgctga acaaaagcca
     2221 atcacctttc gccaatacag cagctacaga gcagccagct ggcgatgagc tgaaggccgc
     2281 acaggctgaa gaaccgcaag aagaaatcga cccactgaag cacacgatac gcgtggaaaa
     2341 cggcgacacg ctgtccacgg tatttgccaa ggtgggcctg agcgcaacca ctttgcatga
     2401 cgtgcttaat agcagtaagg aagccaagca gctgacacgc ctgaaagtag gccaagcgct
     2461 tgagttcaac ctgaataccg acggccagct ggaaagcctg cacagcaagc tcagcgacct
     2521 ggaaagcatt gccctgacga agagcgagaa gggcttcagt ttcaagcgcg aactggtcaa
     2581 acctgaagtc cagaccacct atagccacgg cgtgatcaac agctcgctgt ttctctcggc
     2641 caaacgcgcc ggcctgtcgc acagtctgac catggatctg gccaacgtat ttggctacga
     2701 catcgacttc gccatggata ttcgtgaagg cgacgagttc gagctgatct acgaaaagaa
     2761 agtggtcaac ggcaagcagg tcggcactgg caatatcctc tcggcgcgct tcaccaaccg
     2821 cggcaagacc tacaccgccg tgcgttacac caacaagcaa ggcaatagca gctactacag
     2881 cgccactggc gaaagcatgc gcaaagcctt tatccgcacc ccggtcgatt tcgcccgcat
     2941 cagttcgcgt ttcagcaccg ggcgccgcca tccggtgctg aacaagattc gtgcgcacaa
     3001 aggtgtggat tacgctgccc cacgcggcac gccgatcaag gccgccggtg atggccgcat
     3061 taccctggcc ggccgccacg gtggctatgg caatgcggta atcattcagc acggccaacg
     3121 ctaccgcacc ctgtatgccc acatgaatgg ctttgccaag ggcatccgca gcggcggcaa
     3181 cgtcaagcaa ggtcagatca tcggttatat cggcaccacc ggcctgtcca ccgggccgca
     3241 cctgcactat gagttccagg ttaacggcgt acacgttgac ccactgagtc agaagctgcc
     3301 catggctgac cctatcgcca gcagcgagaa agcccgcttt atgcagttga gcaaaccgtt
     3361 gatggcacaa atggacaaag aaaagtccac catgctcgcc ctcaacaatc agcgctaaac
     3421 catgcccctc tacctcggcg tgatgtccgg taccagcctc gatggcctgg acattgcgct
     3481 gatcgagcag gaacacagca tccgcctgct cggcactcgc tacctaccca tgccagacga
     3541 gctacgcagc gccctgctca gcttgtgcgc atccggcccg gatgaactgg cccgtgcggc
     3601 aatcgccgaa caacagtggg tcgcgctcgc cgcgcaaggc atcagtgagc tgctaataca
     3661 gcacgacata cccgcacaca gcgttcgcgc catcggcagc catggtcaaa ccgtgcgcca
     3721 tgagcctgcc cgcgggttca gcatccagat cggcaactcg gccctactcg ctgaactgac
     3781 cggtatcagc gtcgtcagcg atttccgccg ccgcgatgtt gctgccggtg gtcagggtgc
     3841 accattggtt ccggcattcc accaggcctt gttcgccagc gagggcagca cccgcgcaat
     3901 cctcaacatt ggcggtttca gcaatctcag cctgctggag cctgagcgcc cggtgcatgg
     3961 cttcgattgc ggcccgggca atgtgctgct ggacgcctgg attcaacgcc accagggcct
     4021 gacatacgac caggacggtc aatgggccgc cagtggccag atacagtcgc agcttctgca
     4081 agccttgcta agtgatccct acttccagac tcagggcccg aaaagtactg gccgcgagtt
     4141 gttcaatctg gactggctgg atcagcatct gcagaacctg ccggcgtttg cagcggctga
     4201 cgtgcaggcc accctgctgg aactgaccgc catcagcatt accgaggcgc tcaaggctgc
     4261 tcagcagaac accgagatgc tgctggtgtg tggtggtggc gcgcataacc acacactgat
     4321 gcgccgcctg gcgcagctgc tgcctaatag caaggtgagc agtaccgacg cacatggcgt
     4381 gcccgccgat tgggtcgaag ccatggcctt tgcctggcta gcgcactgct gcctcgaagg
     4441 cattcccgcc aaccggccaa gcgtcaccgg cgcacgcggc ctgcgggtac ttggcgcgat
     4501 ctaccccgcc tgatcgccct gcaaattagc cgccccagaa acgacaacgc cgtgcgcaag
     4561 gcacggcgtt cgcttgaaca gcctacaaat cagatcgaga acgaagagcc gcagccgcag
     4621 gtggtggtgg cgttcgggtt gttgataaca aaccgcgaac cttccagacc ttcctgataa
     4681 tccacctcag cacctgccag gtactggaag ctcatcggat caaccaccag gcttacgcct
     4741 tcgcgctcaa tgatggtgtc atcatcggcc acttcttcat cgaaggtaaa accgtactgg
     4801 aagccggaac aaccgcctcc cgttacaaac acgcgcaatt tcaggcgcgg gttgccttcc
     4861 tcatcgacca ggctcttcac cttgttcgcc gcgccctgcg taaattgcat agcgctgggg
     4921 gtgaaggttt cgacgctcat gctgattaac tcccggcgct acgcgccata ctgcattctg
     4981 atcggcatta tccgcttacc cgagaaaagc ggtcaactat tgtgcagcat c
//
LOCUS       DASRUM010000142        37674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1738199, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000142 DASRUM010000000
VERSION     DASRUM010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 37674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37674
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738199"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VFF22_09370"
     CDS             complement(<1..727)
                     /locus_tag="VFF22_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17055.1"
                     /translation="MKTKKDAVPTPATEATLADLADTLLAPLERPSPLKPPMRLDSSG
                     EQYMYFTERDIDRILDNLDGLRSLVFPPAPQDDDSERRVQHFPSVCLIGLGRCGSNIA
                     LDVASLVYNARQFYLSEFNNEEKAVAEQDFRPTRWIRSNLLRAPSKDSKPVFLIEPLV
                     MLGDLDKDIEGRIRFSRKGETSGFLSDYSKMKIMDLSEVHAGGAGNAPILGQYLAKII
                     LNKDTQRFTNEDWKFIHSYLIDSC"
     gene            1037..2368
                     /locus_tag="VFF22_09375"
     CDS             1037..2368
                     /locus_tag="VFF22_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX17056.1"
                     /translation="MQQQSLQATNAWRILFLLFLANLFNFFDRTIPAIIIEPIRMEWD
                     LSDLQLGLIGTAFTIVYAIAGIPLGRMADTGARRKIMGWGLAVWSGLTAVNGLAWNFW
                     SFLLIRMGVGIGEASYAPAANSLIGDLFPAHKRARAMGIFMLGLPIGLLLAFFTIGAM
                     VKAFDSWRAPFFIAAVPGLILALFMFFIKEPKRGAAELVQSRTTPVEKPLRKVLAIRT
                     FWWLVMAGLAFNFATYACNSFMVPMLQRYFLMPLEQAAMATGVIVGLTGLVGLTLGGW
                     LADKIHQRWQTGRLLFAALSMLIASVATGYALLAGRVEIAVFVALFSIGWLFAYNFYT
                     CVYTAIQDVIEPRLRATAMALFFAGLYLLGGGLGPLAVGMLSDHYSHAAMLAAGTSEM
                     NETFKAVGLHNAMYLIPVALMLTMVALLQASRCFVADAAKMQQGMTEAVPA"
     gene            complement(2420..5263)
                     /gene="rapA"
                     /locus_tag="VFF22_09380"
     CDS             complement(2420..5263)
                     /gene="rapA"
                     /locus_tag="VFF22_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005732270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HZX17057.1"
                     /translation="MAQYEPGQRWISDSEAELGLGTVLMQDGRMLTVLYPATGETRQY
                     ALRNAPLTRVRFSPGDEITHFEGWKLTVQEVDDVDGLLVYHGLNAQHQSVTLPETQLS
                     NFIQFRLASDRLFAGQIDPLPWLALRYHTLDYTSRQLQSSLWGLGGVRAQPIAHQLHI
                     AREVADRIAPRVLLADEVGLGKTIEAGLVIHRQLLSGRANRVLILVPENLQHQWLVEM
                     RRRFNLDVALFDEERFIESDASNPFEDCQLALVALEWLCDDEKAQDALFAAGWDLMVV
                     DEAHHLVWHPEHASPEYALVEQLAGVIPGVLLLTATPEQLGQDSHFARLRLLDPNRFH
                     DLAAFRAESEHYKPIAEAVQELLDQGRLSDAAHKTIHDFLGAGGEELLAAVNAGDSEA
                     SARLVRELLDRHGTGRLLFRNTRAAVQGFPDRELHPYPLASPDEYMELPLGEHADLYP
                     EVSYQAQPDVDEEQRWWKFDPRVEWLIDTLKMLKKFKVLVICAHAETAMDLEDALRVR
                     KGIPATVFHEGMSILERDRAAAYFADEEFGAQVLICSEIGSEGRNFQFAHHLVLFDLP
                     AHPDLLEQRIGRLDRIGQKHRIQLHVPYLENSPQERLFQWYHQALNAFLATCPTGNAL
                     QHQFGSRLLPLLESGDDGEWQTLVDEAKTERIRLEGELHAGRDRLLELNSGGAGEGEA
                     LVEAILEQDDQFALPIYMEQLFDAFGIDSEDHSDNALILRPSEKMLDASFPLGDDEGV
                     TITYDRDLALAREDMQFLTWEHPMVQGGMDLVRSGSMGNTGVALIKNKALKPGTVLLE
                     LLYVSEVVAPRALQLGRYLPPIALRCLLDANGNDLAAKVSFEKLNEQLEPVPRASANK
                     FVQAQRDSLNPLINAGEGKVAERHATRVDEAKRRLAAETDEELARLIALQAVNPSVRD
                     SELNALRQLREQGLAMLDKAALRLEAIRVLVAG"
     gene            5438..5737
                     /locus_tag="VFF22_09385"
     CDS             5438..5737
                     /locus_tag="VFF22_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HZX17058.1"
                     /translation="MLPPIPHSLVPVTVQQDVVKPRPEIPPVTATAESSKESGVSLDK
                     RHPQEAEALLREEQRRRQRRRGYSPEELAEGEVAEADEAVISELPRQGLWVDVEV"
     gene            5749..6351
                     /locus_tag="VFF22_09390"
     CDS             5749..6351
                     /locus_tag="VFF22_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HZX17059.1"
                     /translation="MDLFADSPLQLPDADLQLHAQWLDMATADHWLAELSAQTPWQQP
                     QVQIYGRQLPVPRLVAWYGDAEACYRYSGLTHQPLPWTPLLTMIRAQVEQAVGQPLNG
                     VLLNYYRDGQDSMGWHSDDEAELGANPLIASLNLGGTRRFDLRRKGQSRIEHSLPLQH
                     GSLLVMRGPTQHHWQHQVAKTRSPCAPRLNLTFRLIKPTP"
     gene            6348..6509
                     /locus_tag="VFF22_09395"
     CDS             6348..6509
                     /locus_tag="VFF22_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017906116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17060.1"
                     /translation="MSKDDKLIDFAAERDKRIHDLRDKKLNEMRDAFEQALPLPKGKK
                     KPKGKPKRP"
     gene            6666..6782
                     /gene="ccoM"
                     /locus_tag="VFF22_09400"
     CDS             6666..6782
                     /gene="ccoM"
                     /locus_tag="VFF22_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit CcoM"
                     /protein_id="HZX17061.1"
                     /translation="MFIDVVVLAGIGTVGLMVAFFAGVGYFIWQDSHKRKQG"
     gene            complement(6870..8801)
                     /locus_tag="VFF22_09405"
     CDS             complement(6870..8801)
                     /locus_tag="VFF22_09405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX17062.1"
                     /translation="MPKQWPEIFLIIALLTVCIPAATATELPVHITTAHILNIPCQDF
                     SAPPQVVNEQLLPDKWSTVNLPHALTRQLIPSSALHEHERTATIETWYRLQLPAPASE
                     TQKQLYIPRWKTDGNLAIYADGQLIYMSSGGVYWNGWNIPLSIPLNATANTSSPSTIL
                     LRIQRPSDSGGGISSIWVQESSSLNWRYRLRYLLQVQLPYTSSVVFLAVGLFSFFVWC
                     RLRSEHSYLLFFFISIASFIRTLHYYVGERELSIAESWFSWLTINSLFWMVLVTHFFL
                     QSLHHRPSRWLSALVILVTIVAGVLTLPMLSGLLNAYALSPLIYFALIITGSLVAGFG
                     AYKSKQENCNDGLLLSGWAIAGMLLGCHDWLLQNNYLSIESIYLGPYSNIGAFLIVMS
                     IIYRRFLKANESVKSTNASLAFRLQERENELKNSHQRLREIEHRQTLSNERQRLMQDM
                     HDGMGSSLIIALLEAEKGHLDASSLTDVLKNCIEDLKLTIDSMEPVEADLLLLMATLR
                     FRLTPRLESAGIRLRWDIQNVPALDWLDPRNALHILRILQETFSNIIKHTNATEIRIT
                     TSSDATHVRVIITDNGQGFTAEQSALFNGKGLKNQLRRAASIGAEILWHSTTAGVTVT
                     LTLPIVATKRHSKSAECLP"
     gene            complement(8794..9447)
                     /locus_tag="VFF22_09410"
     CDS             complement(8794..9447)
                     /locus_tag="VFF22_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZX17063.1"
                     /translation="MILVEDDVTFQQALRHTFSTATDMQLVAVLSTRAEGLKMLNQAP
                     ADVLLVDLGLPDGSGIDVIRAAHSKWPECNIMVSTAFGDEMHVMQSLEAGATGYLLKD
                     SSTASIASEIRSLHQGGSPISPLIARQILMRFRHEDTSQPLAATSPSPSRARLSAREQ
                     QVLEYITKGFNSDEIAELMGVSRHTVLTFVRRIYKKLEVSSKTEAIYEARSQGLLGA"
     gene            9739..9996
                     /locus_tag="VFF22_09415"
     CDS             9739..9996
                     /locus_tag="VFF22_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17064.1"
                     /translation="MSIKLRARQLGQGMTEYIIIVALIAISAIVVYNLFGSTVREQVG
                     DMAAELGGGEATQAAATTGGEAQTAAGVKSNLSSFKQDERK"
     gene            10048..11988
                     /locus_tag="VFF22_09420"
     CDS             10048..11988
                     /locus_tag="VFF22_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17065.1"
                     /translation="MVFGLFFLGIALVGLILMYNNGVLTRDRVQVENAADAAVYSQAK
                     LFARHQNLIAYTNRAIVANEMSVGQVVALMSWSKRYANIPRWVNTFAPYRIPLVPAPN
                     PTIADALSAITLPYQILGSVTGVVAKPLLGIYPDVISSFNLVMGFFQKIFAVATLEAQ
                     FTTPINIVDRHEMPGTNDNLKVVTLSHLLLTQNFILTYFASELNLSSLASQAKSASTA
                     GGGSAASSEDFVADFLGSLAPSTMLVNTAPEKYDSSNAQNADNSAARTAGRQYAAIMN
                     SNRNTWLAARNFNPTVSLSPPELPIYLGFITIRLGLNVEVGVFNNGGTAYVYNPKSTA
                     AGGKGIPQYGWSSVDFSSIGFKMKVSLRIEFCLPLLGCFEILTGFPLQMGLPLSGGTH
                     QIVAKAGDQFLIGTQWGSPMQNSPPGSSAPYGEAMQSVHATTWAHGNVLGAFGINQAE
                     DVNTGYGGAPSFFSLGHDYQGRGTSNEFTVAVAKPLSSLRTSDNPASLGLDSGTFALE
                     TQEVKDNALSSLWGGPDHMMTIASAESYFAPPPGRVETPNQFSPFWDARLREPSRVVQ
                     MIAAGQIDLMAVLGLQPGASTSAIAGAVIDRGLKGLINPGKDRVVDKLPSVIKGPVKP
                     VLDKFVDTITSSAGSAVSELVK"
     gene            11991..12902
                     /locus_tag="VFF22_09425"
     CDS             11991..12902
                     /locus_tag="VFF22_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17066.1"
                     /translation="MLASTGRARMHGQAMAEFVVMVAGGVLLLFVLVPVVAKLADMAY
                     TAQQVARYVAWERTVWFDTDKAPGETDDGDTAQRGNEAILKTAEKRLMAYSAAIQPFT
                     AADIDGSADTTSNNSLWRWTHGGEAKPMTATGSMPSHSSLNAEDTPSYSYDVIGIYND
                     AMGGVMKVLSFLKISKDDDFLQVAHETENLYNPSIVIPVSMAGSRLGSKPLFGGALDK
                     QLNIRAQSAVLADGWNAQGDHHFKERADDFAVGTMLSNPLIETIISTIGMFEPSFKDV
                     EFDYVGIKPIPDKPAKCTTSTGFCYFE"
     gene            12906..13571
                     /locus_tag="VFF22_09430"
     CDS             12906..13571
                     /locus_tag="VFF22_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17067.1"
                     /translation="MKIITPLLLSLLLAVNSAQAECNYDDFPLMPDMLVANIGSNIQW
                     NHTPMAGRSFRVPANLASVKAFYDKAWADEVDFSAFNGWEQILHINQQCMMLIQVTRQ
                     NDRYSYGQLLITNPPEKAAAAQVLGDGMPMPPGAQVISDMRSDDDIRQGRMVLLLNEE
                     DMHTTARWYETELKQQGWNLDQRSQQANGVVLSYSRKRELMTVGLLRHQDKTQILVNR
                     MDR"
     gene            13571..14599
                     /locus_tag="VFF22_09435"
     CDS             13571..14599
                     /locus_tag="VFF22_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17068.1"
                     /translation="MKAQRGQSMTEYLVVLGVTGAALMLAGNDVERLFDSLRSSYKTY
                     SSEMNKVQLYNNEAVRILEPSPEPPFDDGQPEPLPPTETELPTNDALAVLNEVYDANG
                     VYVGRVVNGQLVAANGDVIGTYVLDASGNSSLVDANGQVLYPGFSISQVFVDSNGKPL
                     VLQALANANGEVLGFAYLEGKKYFNAITGKALVKQPEGLTPIATRDVLMYAPDGKTFV
                     AAYEAAGLVYAKSDALISSSDTFTSPRKITGELVSLYLAPETEAAWSKYSLCIVRSYG
                     WAAEQVSTLPAKGASVAIGSNVSDQFNAPAPQLSYVDSTADACRARFSVLQTKDPVIG
                     TQWSLRRN"
     gene            14710..16740
                     /gene="cpaB"
                     /locus_tag="VFF22_09440"
     CDS             14710..16740
                     /gene="cpaB"
                     /locus_tag="VFF22_09440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HZX17069.1"
                     /translation="MSSVSGKKLLIIAVACGVLAGAMGWLYLKSKEAQYRDAYRPPVE
                     QKVTVVVPKADIGKAQALTPELVATLDVPSDYLASNVVLAEDWPKLEGRMTVAPLQRG
                     RPISWDAVEQERVSRFSENVELGKRIKTVKISKINSFDGMLRPGDRIDLLGSFAAGDI
                     GLQKQPNYADDVVINILEDIIVLAAGREDASGRKYENYYDQSTPDGFNMNFSTLSLML
                     TPVQVARVELAEKAGEMVAVLRHPKDTSMAELGQVTVANLLDPPPQELIDAVVDADGN
                     LIGRIVGDNIVGADGRIVGKVVDGQAVGFDGNVIGRTVRGLNADDPLLRMHERATVVR
                     DEQGNVIGRVVDGKVIDASGNAIGTVVDGKAVGVDGKSLGRVQANAVLDSQGRVVDMS
                     RSRVQDHAAPQNQERQVVRDATGKVLGRVDGDKIVDAQGQVIGHQRDGKAYDLNGKPL
                     GSISTVVQDAQGKIIGEMGQIARDAEGKIIGRVVDGQLIGADGDVLSVVKDGQVLGQV
                     ENIQLDSSGRPVEGNVRVVRDASGKVLGTLVGDDIIDAQGRKVGSVKNGEVRDLAGQV
                     VAGAQVAVQGDAKVVVRDAEGNVIGTLRGDTVFGADGKATGTLLNGKLVDGNGNVLVS
                     GLTVAAEQAANLPSEQSGKARNKRLEPSRMIQFIPGGTGTAGVIPVQTLRLE"
     gene            16847..18298
                     /locus_tag="VFF22_09445"
     CDS             16847..18298
                     /locus_tag="VFF22_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HZX17070.1"
                     /translation="MALQVQADEIPAAMVLYDGDVHVILAPGVERVAVGNVAIISATM
                     LKNEEVVITAETPGETTVQLWFADDQRQQVSVVVAKANGYRQVGELRALLSDIPGLRI
                     RTVGRQVVLEGRLSSEYLARVKEAAKFYENVLVLAEEQSGAATKADSAQVLQEVKAML
                     GQIPGIQIKAVGRQIVIDGDLDAIDITRIELLKERYPDLLVLAQPMSKFLAPMIYFDV
                     RITEFAKDDVEELGVNWSTSINGPTFAFNADGGTNNLYRGQFPSASGTFDDLNDAVGN
                     VGSHAYWGVASEITSRINLLEKNGSALTLASPRLSARSGGKAELTVGGQVPVVTSSIN
                     GPSVEYKDFGILLNIEPQLYGGETIATNVKAEISQLDKANQVGEYPAFKTRRTENEVQ
                     LKVGETLVLSGLVTEDSQSTIEGIKWLMNIPYLGGLFRNKSYKGNRTELVIFITPRLL
                     TEGADSPNVKELAREKQMIEKYAKTVGNLELID"
     gene            18308..19945
                     /locus_tag="VFF22_09450"
     CDS             18308..19945
                     /locus_tag="VFF22_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013694015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZX17071.1"
                     /translation="MFEVVVTYKGGEQLDTLTCTSAQCEVGKARTGLVRLRGWKVAPN
                     HARIEQSLGGLFVENISRGNELLVNGKPVERFGPLSPEDLIGIGAYNLQVRGLGQIET
                     EADTPLPPDAAASSGEDNAPYMEWSQYVQAELFRAMDLRRMNLDSMGPLEVRNSLSEL
                     INGITEQIAHRLPATLSREILHKIVINEAVGLGPLEDLLADPSVSEIMVNRYDDIYVE
                     RSGRLERTGISFSSDSAVLSTIERIISPLGRRIDESSPMVDARLKDGSRVNAIIPPLA
                     LRGPCLTIRKFSEKKLTTDDLLGYGSINEPMVEFLRIAVEQRMNIVVSGGTGSGKTTL
                     LNILSNFIPLDERVVTIEDAAELKLVQPHVVSLEARPANMEGKGQVTIRDLVRNCLRM
                     RPDRIVVGECRGGEALDMLQAMNTGHDGSLTTGHANSPRDMLRRLEVMVLMAGMDLPV
                     QAIREQISSAVQLIVQQTRFGDGSRRITSITEVTGMEQSTILLNEIFAYQQQGFDEQG
                     RVKGHYRATGQIPEFYEALSKRGIGVNMDIFHTPERL"
     gene            19946..20803
                     /locus_tag="VFF22_09455"
     CDS             19946..20803
                     /locus_tag="VFF22_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZX17072.1"
                     /translation="MAAVWLVLCALLLAVSVCALALLISRRVADNLDHYRRQFSRRAE
                     LEMADMFIFTSGRQVLILTLVLLLLVPVLLELLFQLPVVTAAGIVVTLFTPHLMFNHL
                     RKARLKKFEEQLPDAFMLLSSSLQSGASLNMALENVVHQSPAPLNQEFGLLIKNIRLG
                     VTLEEALIKLEKRLPLPSFIMASSAIRISREVGGNLVETINGMAAMLRRKKVMEGKID
                     SLTAQGRAQGTFMAMLPIMLAIILSFIEPEAMRQLYTTRNGLMVLAVMVVMEVLGFMF
                     IKKITRIDA"
     gene            20806..21684
                     /locus_tag="VFF22_09460"
     CDS             20806..21684
                     /locus_tag="VFF22_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZX17073.1"
                     /translation="MSELLVLFSSASLGVTVTCILLAVLSLFTLLPAENRTYMDPVPS
                     WIRPIWPLVRLVSHFACSNLPPKLMDWLDVRMQRTGALYVLTAEDFLALCITMALLFP
                     VLAILPMASGKSGILWSVVLLMLLMGAALPVMWISDTHKRRDRDIVRNLPVFLEYLSM
                     CVDAGLNFLGALQQAVDKGPDGAMKNEFRIVLRDIRSGLPRAEALARMEQRINLKDVT
                     TFVRSVVQAEKMGSSLRNTLQIQAQQRLEERFQRAEKTAMQAPVKLIIPLIMFIFPLT
                     FVILLFPIVVKFMGQT"
     gene            21684..22037
                     /locus_tag="VFF22_09465"
     CDS             21684..22037
                     /locus_tag="VFF22_09465"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HZX17074.1"
                     /translation="MREALVVHTAGNRPPSLLQLQLAHTPWARLRGLLGYKTLPAGHG
                     LWLRPCNSVHCWFMRFAIDVIYLDKQQRVLQIRTQLRPWQLSLCWPANSVIEMAAGEC
                     QRLNITVGDQIQCVA"
     gene            22025..22477
                     /locus_tag="VFF22_09470"
     CDS             22025..22477
                     /locus_tag="VFF22_09470"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZX17075.1"
                     /translation="MRGLIIIALSVVLQGCAGLGLGSSTDLLDLERQAQQAYAQGNYA
                     RSSEYYAKLAAQMPTDADTRYRLANSLAREGNHALAIRTYREALLRDPQHAGAWYNLM
                     QLQAQELTLTAMEARRYLDRKNPRAQPALERAEQLIEVFDAHAPTRSE"
     gene            22449..23174
                     /locus_tag="VFF22_09475"
     CDS             22449..23174
                     /locus_tag="VFF22_09475"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HZX17076.1"
                     /translation="MRTPPPAANSQQERGQAMVEFLIIIPVLILLIFGAFQATLIYSA
                     KSGLNYAAFQAARLGAVNNAQYEGMRRGLLRGMYPMFSQYPEATRMEKTAEEVDNFVL
                     ITRISPALNSFTAWQIPHDGLRSAGITAQAIPNDNLMYRSAQQNPVSIQDANLLKIRV
                     QYCVKLIVPMVENLLSSASKFNQRQSGGSFSENSRQSWDQYAAICGQRKGFVLTSEAT
                     VRMQSAAVHDTENCGAGKKMLCP"
     gene            complement(23184..24725)
                     /gene="glpD"
                     /locus_tag="VFF22_09480"
     CDS             complement(23184..24725)
                     /gene="glpD"
                     /locus_tag="VFF22_09480"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HZX17077.1"
                     /translation="MPTRPASNTPLAEVYDLAVIGGGINGVGIAADAAGRGLSVFLCE
                     KDDLAQHTSSASSKLIHGGLRYLEHYEFRLVREALAEREVLLTKAPHIVKPMRFILPH
                     RPHLRPAWMIRAGLFLYDHLGKREKLAGSRSLRFGAGSPLQAEITRGFEYSDCWVDDA
                     RLVVLNAMAAREKGAHVHSRTRCVSARRSKGLWHIHLERSDGSLFSIRARALVNAAGP
                     WVARFIREDLKQESPYGIRLIQGSHIVVPRLFDGEQAYILQNEDRRIVFAIPYQERFT
                     LIGTTDREYQGDPAKVAISDEETDYLLNVVNAHFKKQIQRSDILHTYAGVRPLCDDES
                     DDPSAVTRDYTLSLSGHPGEAPLLSVFGGKLTTYRKLAESALAQLAPFFPKMGPSWTA
                     SSLLPGAEQLDSQSALVEALCARFGWLPTSLARRWASSYGSRSWVMLKGVSNLSDLGE
                     HLGGGLYTREVDYLCQEEWAMQADDILWRRSKLGLFTTPAQQAKLDNYLRIESPRSPE
                     DAHAA"
     gene            complement(24861..25619)
                     /locus_tag="VFF22_09485"
     CDS             complement(24861..25619)
                     /locus_tag="VFF22_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HZX17078.1"
                     /translation="MTLPPRQHDILELARERGYVSIDELAQAFAVTPQTIRRDINQLA
                     EQGLLRRTHGGAASESSSTQNTAYSMRAGQMRDEKQRIAAAIAAQIPNHASLFINIGT
                     TTEAIARELLGHKGLKIITNNLHVAAQLSAKADFEVLLAGGTVRSDGGIVGQAAVDFI
                     QQFKVDFALVGISGIDEDGSLLDFDYQEVRVSQAIISNARQVFLAVDSSKFGRNAVVR
                     LGSVALVNRVFTDAAPSAAINRLLAEHKVQLDLV"
     gene            25718..26188
                     /gene="ybaK"
                     /locus_tag="VFF22_09490"
     CDS             25718..26188
                     /gene="ybaK"
                     /locus_tag="VFF22_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HZX17079.1"
                     /translation="MTPAIDLLKKAKAVHQVHSYHHDPKAASYGLEAAEKLNLEPAQV
                     FKTLLAASEKGELLVAVVPVAGTLDLKALAQAAGVKKADMADPAAAQRATGYLLGGIS
                     PLGQKKRLRTFIDESAQQFPNIYVSAGRRGLEVQLDAAVLAQLTQALFAPIGRN"
     gene            26227..27714
                     /gene="glpK"
                     /locus_tag="VFF22_09495"
     CDS             26227..27714
                     /gene="glpK"
                     /locus_tag="VFF22_09495"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HZX17080.1"
                     /translation="MSHYLLAIDQGTTSSRAIVFSAQGLPVARAQQEFKQYFPQDGWV
                     EHDGEELWLSTLKVCREALKQSGLSASDVAAIGITNQRETTLVWDAQTGAPIHPAIVW
                     QDRRTADYCAQMKAAGHEAAVAAKTGLLIDPYFSATKLRWILEKVPGARERAERGELR
                     FGTVDSFLLWRLTGGKSHKTDASNASRTLLFNIHTQQWDEELLKLFEIPASLLPEVLD
                     CAADFGITEANLLGAAIPVLGMAGDQQAALIGQACFEPGMVKSTYGTGCFMIQNTGSV
                     PVISKNRLLTTVGYRLNGKVTYAVEGSIFVAGAAVQWLRDGIKLISHARDSEAMAEQT
                     GDACGVYLVPAFTGLGAPYWDPKARGAIFGLTRDTGIKEIVTAGLQSVCYQTRDLLEA
                     MRQDGAAEPSALRVDGGMVENNWVMQFLADILGVPVERPEVTETTALGVAYLAGLQAG
                     LYKDLASIARHWHRQQRFDPRMADSHRSKLYDGWLDAVKRVRSEG"
     gene            complement(27924..28706)
                     /locus_tag="VFF22_09500"
     CDS             complement(27924..28706)
                     /locus_tag="VFF22_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014595843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HZX17081.1"
                     /translation="MNRRLIIPLILLCSLLSPAAWATINAAEAMNLSGMQRMLSQRIA
                     KSYLMLGAEIRPEQAEQQLDQSIAKFESNYLALSEYAPTAEIRSALQEAGTTWQRYRE
                     LALSRPDKPQALLVLELSDQLLAQSEQVVQLLERHSGGRSAVLVNRSGRQRMLSQRIA
                     KLYLAISWRLPVANLQQSFEQAVGEFEVALQELQQASQNTAQISKGLNQAEAQWRFTR
                     AGFNLASDSRYVPTIITTTTETLLWQMNELTNQYEGVMLTGS"
     gene            complement(28781..30013)
                     /locus_tag="VFF22_09505"
     CDS             complement(28781..30013)
                     /locus_tag="VFF22_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HZX17082.1"
                     /translation="MMKLSLLSLAIAGTTLSHAAFAEESFSNLFSQAKPIFDARYRYE
                     HVDQDNALKHANAQTLRTRIGVQSGKWYGLSALIEADNVSRIGDASYNSTRNGQGDYS
                     VVADPDGSEINQALLRYDHQYGSAVLGRQRINLDNQRFVGGVAWRQNEQTYDGALAQL
                     KPLAGLTLTYAYIDNVNSIFGPGDNRFDTAANPANIEGHSHLLNAQYVVMPELTATAY
                     QYRLGLDNLTAGSQSSKTTGLRLNGAIAGVSYALEYAKQSDLSDYADNTQDLDSEYYL
                     AELGYSFKGVAFKGGYEVLSGDSGPGNRAFQTPLATKHAFQGWADIFLLTPADGVKDA
                     YVGLTAPLLGGSLQAWYHDFRAERDSSQYGEEIDVSYAHPIPGVKGLVGLVKYANYDA
                     DDFSVDTEKLWAQLQYSY"
     gene            30361..31161
                     /locus_tag="VFF22_09510"
     CDS             30361..31161
                     /locus_tag="VFF22_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019093251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HZX17083.1"
                     /translation="MQLEFHQVDAFATQPFTGNPAMVYRLDNWLEDGLMQQIAAEHNL
                     AETAFVVKEGAAWHIRWFTPAAEVPLCGHATLASAHVLFEVYGEPGDVLDFTCLSGAL
                     QVSRAGDKLVLDFPVRRVQPCELRADVEQALGQPVESVLALHEGPGVQELLAILPNEA
                     AVRTCQPDLAAVARLPGLGLLISAPGERHDFVSRYFAPTVGINEDPVTGSTHCMLTPY
                     WAQRLDKSQLSAFQCSARGGELHCELDGERVKIAGQALLIASGRLFLP"
     gene            complement(31166..32272)
                     /locus_tag="VFF22_09515"
     CDS             complement(31166..32272)
                     /locus_tag="VFF22_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019411003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX17084.1"
                     /translation="MNQPLLNLRELSCGYPEHKVVQNLNLHLNAGDIGCLLGPSGCGK
                     TTTLRAIAGFEPVLEGEIQLADQVISKAGFTLAPEKRRIGMVFQDYALFPHLSVAENI
                     GFGIRKQPNCERVTQELMELVKLGHLGKRYPHELSGGQQQRVALARALAPEPQLLLLD
                     EPFSNLDGELRRRLSHEVRDILKARGTSAILVTHDQEEAFAVSDHVGVFKEGKLEQWD
                     TPFNLYHEPLTPFVASFIGQGYFIRGQLLSPDSVQTELGLIRGNRAYTWPTGSAVDVL
                     LRPDDIVYDPDSTQKALIVGKTFLGAATLYRLQLPTGSQLESIFPSHADHLPGQQVGI
                     RVAADHLVVFPAPGSIAAQVNLGESGVRRYSSDS"
     gene            complement(32269..33189)
                     /gene="argF"
                     /locus_tag="VFF22_09520"
     CDS             complement(32269..33189)
                     /gene="argF"
                     /locus_tag="VFF22_09520"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZX17085.1"
                     /translation="MNARHFLSLMDCTPDELVGLIRRGMELKDLRNRGVLFEPLKNRV
                     LGMIFEKASTRTRLSFEAGMIQLGGQAIFLSPRDTQLGRGEPIGDAAIVMSRMLDAVM
                     IRTFAHSTLTEFAANSRVAVINGLSDDLHPCQLLADMQTYQEHRGSIKGKTVAWIGDG
                     NNMCNSYIEAAMQFDFQLRVACPAGYEPNAKFLAAAGDRVQILRDPLAAVAGAHLVST
                     DVWASMGQEDEAAERLKLFQPYQVNRALLDAAADDVLFLHCLPAHRGEEISEDLLDDP
                     RSAAWDQAENRLHVQKALLEFLVEPAYHHA"
     gene            33522..33953
                     /locus_tag="VFF22_09525"
     CDS             33522..33953
                     /locus_tag="VFF22_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017682588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17086.1"
                     /translation="MKHLASIAAISAFALLTGCASQNVSQPTLPLGGAVETGLKADVK
                     VGEIITGESSVNILFSVFKLGGDTQFADGVTYGGGEGGFALGLDPVASAKSAAAYKAV
                     KGSGADVIVAPRYEVSVQDYFVYKTVNVKVTGNKGTVTAIR"
     gene            34097..36205
                     /locus_tag="VFF22_09530"
     CDS             34097..36205
                     /locus_tag="VFF22_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HZX17087.1"
                     /translation="MTKSLHHRACHLCEAICGLSIETETQPDGTVQIRSIKGDAQDSF
                     SRGHICPKAVALQDIQSDPDRLRQPMRRVGNEWQAIDWDDAFELVAERLSTIQTRHGQ
                     NAVAVYQGNPSVHNYGLMTHSNYFLGQLKTRNRYSATSVDQLPHHLTSHLMYGHGLLI
                     PIPDIDHTDFMLILGGNPLASNGSIMTVPDVEKRLKAIQKRGGKLVVVDPRRSETAAI
                     ADQHVFVRPGQDAALLFGLLNTLFEENLGRASHLAIDGLDEVRQAIAGFTAESMSAQC
                     GVPAQTIRQLARDFAAAESAVCYGRMGVSTQAFGTLCQWLVQLINLVTGNLDRVGGVL
                     CTEPAVDLVASTSGGHFNRWQSRVSGLPEYGGELPVSALAEEMLSEGEGQVRALVTVA
                     GNPVLSTPNGRQLEQALDGLEFMLSIDFYINETTRYADLILPPTGPLEHDHYDTTFNM
                     FAVRNVTRFNEAVLAKPAGALHDWEIFIGLAKAFAAKTGVELKPTMAPEQMIDMGLRF
                     GTYGDKSEHKLSLAALREHPHGLDLGALKPNLIGRLKTANKQIQAAPAVIMADLQRFA
                     EQPAAKADELLLIGRRHVRSNNSWMHNYHRLVKGKPRHQLLMHPADLSQRGLSDGQLV
                     RVRSRVGVIEVEVAASEDMMTGVVSLPHGWGHARPGVQMSIASEQPGASANDLTDERQ
                     LDALSGNAALNGVPVKVEAA"
     gene            36326..36652
                     /gene="grxD"
                     /locus_tag="VFF22_09535"
     CDS             36326..36652
                     /gene="grxD"
                     /locus_tag="VFF22_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HZX17088.1"
                     /translation="MDIIETIKEQIAKNTVLLYMKGSPNAPQCGFSSKAAQVVMACGE
                     KFAYVDILQNPEIRANLPKYANWPTFPQLWVGGELVGGSDILAEMFEKGELQTLIKDA
                     VQKADA"
     gene            complement(36721..37191)
                     /gene="bfr"
                     /locus_tag="VFF22_09540"
     CDS             complement(36721..37191)
                     /gene="bfr"
                     /locus_tag="VFF22_09540"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003289285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HZX17089.1"
                     /translation="MKGDKKVIQHLNKILGNELVAINQYFLHARMYEDWGLKKLGEHE
                     YHESIDEMKHADKLIKRILFLEGLPNVQDLGKILIGENTQEMLECDLKIEHKAHIDLK
                     AAIAYCESIGDYASRELLEEILCSEEDHIDWLETQLSLIAAVGLQNYLQSQMDE"
     gene            complement(37450..37668)
                     /locus_tag="VFF22_09545"
     CDS             complement(37450..37668)
                     /locus_tag="VFF22_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HZX17090.1"
                     /translation="MYVCLCEGVTDGQIRDAIYDGCCSYRDVRASLGVASQCGKCACL
                     AKQVVRETLSDVQNGQAAMAYPASFVAA"
BASE COUNT         7499 a        10889 c        11645 g         7641 t
ORIGIN      
        1 cgcacgaatc gatcaggtag ctgtgaatga atttccagtc ttcgttggta aaacgctggg
       61 tgtccttgtt aaggatgatc ttggccaggt attgaccgag gatcggcgcg ttaccggcgc
      121 cgcccgcatg tacctcggag aggtccatga tcttcatctt gctgtagtca ctgaggaaac
      181 cgctggtttc gcccttgcgt gaaaagcgga tgcgcccttc gatgtccttg tccaggtcgc
      241 cgagcatgac cagtggttcg atcagaaaga ccggcttgct gtccttgctc ggtgcgcgca
      301 gcaggttgct gcgaatccag cgcgtggggc ggaaatcctg ctcggccacg gccttttctt
      361 cgttattgaa ttcgctcagg tagaactgcc gagcgttgta gaccagcgat gcgacatcca
      421 gggcgatgtt cgagccgcag cggccgaggc cgatcaaaca gaccgacggg aagtgctgca
      481 cccgccgttc gctgtcgtca tcctgcggcg ctggagggaa caccaggctg cgcaggccgt
      541 ctaggttatc gaggatgcga tcaatgtcgc gctcggtgaa atacatgtac tgctcgccgc
      601 tgctgtccag tcgcatgggg ggcttgagcg gggaggggcg ttcgagcggg gcgagcaggg
      661 tgtcagccag gtcagccagt gtggcttcgg tcgcgggggt gggcacagcg tctttttttg
      721 ttttcattta gagacagtcc ggcggctagc gagggtgggc ggaaaatggc cacatttcac
      781 tcaacaagct tgtttgtagc atgaaaaatg aaaaatctcg ctttttcact cgggtttact
      841 gaggcatatc cggcgttttt ccgttgcgct ttgtgacttg cgtgcttgcc cggacgccca
      901 ttacccggca tacacgcaac aaatagtcac agacagcgta gacgcaaggc tctagattgc
      961 gcctgcacat gctggatgcg gcggttggcc acgcgccacc gacctgcttc gataacaaca
     1021 aatagagatg gattccatgc agcagcagtc cctacaagcg acgaatgcct ggcgcatttt
     1081 gttcctgctt tttcttgcca acctgttcaa cttcttcgac cgcaccattc cggcgatcat
     1141 catcgagccg atccgcatgg agtgggatct cagcgacttg cagctgggct tgatcgggac
     1201 cgcctttacc atcgtctatg cgattgccgg cattccgctg gggcgcatgg ccgataccgg
     1261 cgcgcggcgc aagatcatgg gttggggcct ggcagtctgg agcggcctga ccgcggtcaa
     1321 cggcctggcc tggaacttct ggagcttcct gctgattcgt atgggcgtcg gcattggcga
     1381 ggccagctat gcgccggcgg ccaactcgct gatcggcgat ctgttcccgg cgcacaagcg
     1441 tgcgcgggcc atgggcatct ttatgcttgg cctgccgatt ggcttgctgc tggcattttt
     1501 caccatcggt gcgatggtca aggccttcga cagctggcgc gcgccgttct ttattgccgc
     1561 tgtgccgggg ctgatcctcg cgctgtttat gttctttatc aaagagccca agcgcggcgc
     1621 ggccgagctg gtgcaatcgc gcacaacacc ggtggagaaa ccgctacgca aggtgctggc
     1681 gattcgcact ttctggtggc tggtgatggc cggccttgcc ttcaacttcg ccacctatgc
     1741 ctgtaactcg ttcatggtgc cgatgctgca acgctacttc ctgatgccgc tggagcaggc
     1801 ggcaatggct accggggtga tcgtcggctt gactggcctg gtcggcctga ccctaggcgg
     1861 ctggctggcg gacaagatcc accagcgctg gcagaccggg cgtttgctgt ttgccgcgct
     1921 gagcatgttg attgcctccg tggccacggg ctacgccctg ctggccgggc gggttgaaat
     1981 tgccgtgttc gtggcgctgt tcagcatcgg ctggttgttt gcctacaact tctacacctg
     2041 cgtttacacc gcgattcagg atgtgatcga accacgcctg cgcgccaccg ccatggcgtt
     2101 gttctttgcc ggcctgtact tgctcggcgg cggccttggc ccattggcgg tgggcatgct
     2161 ctctgatcac tactcccatg ctgcgatgct tgccgccgga accagtgaaa tgaacgagac
     2221 cttcaaggcc gtcggtctgc ataacgccat gtacctgatt ccggtggcat tgatgctgac
     2281 catggtggcg ctgctgcagg cctcgcgttg ctttgtcgcc gatgccgcca agatgcaaca
     2341 gggcatgact gaggcggtgc ctgcctgacc ccaaaaacaa aaggcccgca tctgcgggcc
     2401 ttttgttatc tcggcgaaat taaccagcaa ccaagacgcg aatagcctcc agacgcaacg
     2461 ccgccttgtc gagcatggcc aggccttgct cgcgcagttg gcgcagggcg ttgagttcgc
     2521 tgtcacgtac gctcgggttg accgcttgca gggcgatcag gcgcgccaat tcctcgtcgg
     2581 tttcggcggc caggcggcgc ttggcttcgt cgacgcgcgt cgcgtggcgt tcggcgacct
     2641 tgccttcgcc agcgttgatc agcgggttga ggctgtcgcg ctgggcctga acaaacttgt
     2701 tggcactggc gcgtggcaca ggctccaact gctcgttgag cttctcgaac gagaccttgg
     2761 cggccaggtc gttgccattg gcatccagca ggcagcgcaa tgcgataggc ggcaggtagc
     2821 ggcccagttg cagagcgcgc ggggcgacta cttcgctgac atacagcagc tccagcagca
     2881 cggtgccggg cttcagcgcc ttgttcttga tcagcgcgac gccggtgttg cccatcgagc
     2941 cggagcgcac caggtccatg ccgccttgca ccatggggtg ttcccaagtg aggaactgca
     3001 tgtcctcgcg ggccagagcc aggtcgcggt cgtaggtgat ggtcacgcct tcgtcatcgc
     3061 cgagcgggaa gctggcatcc agcatctttt cgctggggcg taggatcagg gcgttatcgg
     3121 aatggtcttc gctgtcgatg ccgaaggcgt cgaacagctg ctccatatag atcggcagcg
     3181 cgaactggtc atcctgctcg aggatcgcct caaccaatgc ctcgccttcg ccggcaccgc
     3241 cagagttgag ttccagcagg cggtcgcggc cggcgtgcag ttcaccttcc aggcgaatgc
     3301 gctcggtctt ggcttcatcg accagggttt gccattcgcc gtcatcgcca ctttccagca
     3361 gcggtagcag acggctgccg aactggtgct gcagggcgtt gccggttggg caggtagcga
     3421 ggaaggcatt cagcgcctgg tgataccact ggaacaggcg ttcctgcggg ctgttttcca
     3481 gataaggcac gtgcagctgg atacggtgct tctgaccgat acggtcgagg cggccgatac
     3541 gctgctcaag caggtccggg tgggccggca ggtcgaacag aaccaggtgg tgagcgaact
     3601 ggaagttgcg gccttccgag ccgatttccg agcagatcag cacctgggcg ccgaactctt
     3661 catcggcaaa ataggccgct gcgcggtcgc gctcaaggat gctcatgcct tcatggaaca
     3721 ccgtggccgg gatgcccttg cgcacgcgca gggcgtcttc caggtccata gcggtttcgg
     3781 cgtgggcgca gatcaccaga accttgaatt tcttgagcat cttcagggtg tcgatcagcc
     3841 actcgacgcg cgggtcgaat ttccaccagc gctgctcttc atcgacatcc ggttgcgcct
     3901 gatagctgac ttccgggtat agatcggcat gctcgcccag cggcagctcc atatattcat
     3961 cggggctggc cagcgggtag gggtgcagct cgcgatccgg gaaaccctgt acagcggcgc
     4021 gggtgttacg gaacagcagg cgaccggtgc catggcgatc cagcagctcg cgcaccaggc
     4081 gcgccgaggc ttcgctgtca ccggcattca ccgcagccag cagttcttca ccgccggcgc
     4141 cgaggaaatc atgaatggtc ttgtgcgctg catccgacag gcggccctgg tcgagcagct
     4201 cctgcacggc ttcggcaatc ggcttgtagt gctcgctttc ggcgcggaag gcggccaggt
     4261 catggaaacg gttcgggtcg agcaggcgca ggcgggcgaa gtggctgtcc tggccaagct
     4321 gttccggggt ggcggtgagc agcagcacgc cggggatcac cccggccagc tgttcgacca
     4381 gcgcatattc cgggctggcg tgttccgggt gccagaccag gtggtgcgct tcgtcaacaa
     4441 ccatcaggtc ccagccggcg gcaaacagcg cgtcctgggc cttttcgtcg tcgcacagcc
     4501 attccagggc gaccagggcc agctggcagt cttcgaacgg gttgctggca tcgctttcga
     4561 taaagcgctc ttcatcgaac agcgcgacat ccagattgaa gcgccggcgc atttccacca
     4621 gccactggtg ctgcaggttt tccggcacca ggatcagcac acggttggcg cgcccggaga
     4681 gcagctggcg gtgaatcacc aggccggctt cgatggtttt gcccaggccc acttcgtcgg
     4741 ccagcaatac gcgcggggcg atgcggtcgg cgacttcgcg ggcgatatgc agctggtgcg
     4801 caatcggctg cgcacgcacg ccgcccaggc cccaaaggct ggattgcaac tggcggctgg
     4861 tgtaatcgag ggtgtggtag cgcagggcta accagggcag cgggtcaatc tgcccggcga
     4921 acaggcgatc actggccagg cggaactgga taaagttcga cagctgggtt tccggcaggg
     4981 tgacgctttg gtgctgggcg ttgaggccgt gatagaccag cagcccgtcg acgtcgtcga
     5041 cttcctgcac ggtcagcttc cagccttcaa aatgggtgat ttcatcaccc ggggagaagc
     5101 gcacgcgggt caagggcgca ttgcgcaggg cgtactggcg agtttcgccg gtggccgggt
     5161 agagcacggt gagcatgcgg ccgtcctgca tcagaacggt tcccaaaccc agctccgctt
     5221 cgctgtcact gatccagcgt tgccccggtt catactgcgc catgcttgtc tcccgcgtga
     5281 aaaagccgac tatgttaacg cagcgttggg tcctggccca aggcgcattg ctgctcattg
     5341 ggcactttgt gtgtttaggt agatcgaaag tgtagcgagg cggggctcaa gtcgtggctt
     5401 ggctggccga cagtctgacc aaaggagggc ttaatagatg ctaccgccga ttcctcacag
     5461 cctggttccc gtgaccgtcc agcaggacgt ggtcaagccg cgcccggaga tcccgcccgt
     5521 taccgccacg gccgaaagct ccaaggaaag tggcgtaagc ctggacaaac gccacccgca
     5581 ggaagccgag gcgttgctgc gcgaggaaca gcggcgacgc cagcgtcgcc gcggctacag
     5641 ccccgaggag ttggccgaag gcgaagtggc cgaggcggac gaagcggtga tcagtgagct
     5701 gccgcgccag ggattgtggg tggatgtgga agtttgagtg atttatccgt ggatctattt
     5761 gccgacagcc cgctgcagtt gcccgacgcc gacttgcagc tgcatgcgca gtggctggat
     5821 atggcgaccg ccgaccactg gttggctgag ctgagcgcgc agacgccctg gcagcagccg
     5881 caggtgcaga tttacgggcg gcaattgccg gtgccgcgcc tagtggcctg gtatggcgat
     5941 gctgaggcct gttaccgcta ttcaggatta acccatcaac cgttgccctg gacgccgctg
     6001 ctgacaatga ttcgcgcgca ggtggagcag gcggtcggtc agccactgaa tggtgtgctg
     6061 ctcaactatt accgtgatgg ccaggattcc atgggctggc acagtgatga tgaggccgag
     6121 ctgggcgcta atccgctgat cgcctcactt aatctgggcg gtacccggcg cttcgacttg
     6181 cgccgcaagg ggcagagccg catcgagcat tcgttgccgc tacagcatgg ttcgctgctg
     6241 gtcatgcgag ggccaaccca gcatcattgg caacaccaag tggcgaagac gcgcagcccc
     6301 tgcgcgccac gtctgaacct gacctttcgt ttgatcaagc caaccccatg agcaaagacg
     6361 acaagctgat tgatttcgca gccgagcgcg acaagcgcat ccatgacctg cgcgacaaga
     6421 aactcaatga aatgcgcgat gccttcgaac aggctctgcc gctgcccaag ggcaagaaaa
     6481 agcccaaggg caaaccgaaa aggccttgag gcggctcagt cataggctga tttgttgatc
     6541 tggatcagct aagtacagcc tcatctggcc cgcgcccgcc gcttgttgat ctggatcaat
     6601 agcgccccag cagcccctgt taatttgaag ccatacccac acaggcttca tagcaggagg
     6661 caaccatgtt tatcgatgtc gtagttctcg ccggtatcgg caccgtcggt ctgatggtcg
     6721 ccttcttcgc cggtgtcggt tatttcatct ggcaggattc gcacaagcgc aagcagggct
     6781 gattctgcag taaggggcac gcaaggcaat ttgggcgact tcggtcgcct ttttttatgc
     6841 ctgtaatgct agctagcaag gcttatgttt tatggtaggc actcggctga tttgctatgt
     6901 cgcttagtgg cgacaatagg cagcgttaac gtgacagtta cgccggctgt agtggaatgc
     6961 cagagaattt cagcgcctat tgaagcggct cggcgcagct ggttcttaag tcctttaccg
     7021 ttaaatagcg cggactgttc tgcggtaaag ccctggccat tatctgtgat aatgactctg
     7081 acatgagtcg catcagaaga tgtggtaatg cgaatttccg tagcattggt gtgcttgata
     7141 atgttggaga atgtctcttg aagaatgcgc aagatatgca gcgcattacg cgggtcaagc
     7201 cagtcaagtg cgggcacatt ctgaatatcc cagcgcaagc gtattccggc actttccaag
     7261 cgcggtgtta aacggaatct taatgttgcc atcaacagca ataggtcggc ctctaccggc
     7321 tccatcgaat cgatggtgag tttcagatct tcgatgcagt ttttcagtac gtcggttaat
     7381 gacgaggcgt caagatggcc tttctcagcc tccagcaatg caattatgag tgacgagccc
     7441 ataccatcgt gcatgtcttg cataagtcgc tgacgttcat tgctgagtgt ttgccgatgt
     7501 tcaatttcgc gtaagcgctg atggctgttc tttagctcgt tttcacgctc ttgtaggcga
     7561 aaagccaagc tggcattggt gcttttgacg ctttcattag cttttaggaa acgtcggtaa
     7621 ataatgctca tgacaatcag gaatgcgccg atgttgctgt aaggaccgag ataaatagat
     7681 tcgatactta aatagttgtt ttgcagtaac caatcatggc agcccagcaa catgcctgct
     7741 attgcccagc cgcttaataa caatccatca ttgcaatttt cctgctttga tttgtatgcg
     7801 ccgaatcctg ctactaaaga tccggtgatg atcaaggcga agtaaatcag tggcgacagt
     7861 gcataggcat tcagtagtcc ggaaagcatt ggcagagtca gcactccggc tacaattgta
     7921 accaatatga ctagtgcgct tagccaacgg ctggggcggt ggtgaaggct ttgcaggaag
     7981 aagtgagtaa ctaataccat ccagaatagt gagtttatgg ttagccaact gaaccaggac
     8041 tccgctatgg atagctcacg ctcgccgacg taataatgga gtgtgcgaat aaacgaggcg
     8101 attgagataa agaaaaataa taggtaggag tgctcgctgc gtaatcggca ccaaacaaaa
     8161 aaagagaata agccaactgc tagaaaaacc acgctactgg tatacggcag ctggacctgt
     8221 agtaagtatc gaagccggta acgccagttt aggctgctgc tttcttgcac ccatatagag
     8281 gaaataccgc cacccgaatc gctcgggcgt tggatgcgca agaggatagt gctggggctg
     8341 gatgtgttgg ctgttgcatt taacgggatg ctcaggggga tattccagcc attccagtac
     8401 acgccaccgc ttgacatgta aatcagctgg ccgtcggcat aaatggcaag gttgccatcg
     8461 gttttccagc gtggaatata gagttgtttt tgtgtttctg aggctggcgc tggaagttgc
     8521 aggcgatacc aggtttctat tgtggctgtt ctttcatgtt catgcagggc ggaggaagga
     8581 atcaattgcc gagtcagggc gtgtggcaga ttaacggtag accatttatc aggcagcagt
     8641 tgttcattaa ccacttgagg tggggcgcta aagtcttggc aggggatatt gagtatgtgc
     8701 gcggtggtaa tgtgtacggg taattcagtc gcagtagctg caggaataca tacagttagc
     8761 agtgcaatta tgagaaagat ttctggccat tgcttaggca cccagtaatc cttggctgcg
     8821 cgcttcgtag atcgcttctg ttttcgagct gacttcaagc ttcttataaa ttcgccgtac
     8881 aaaggtcaga acagtatggc gtgatacgcc catcagctcg gcaatctcat ccgaattaaa
     8941 gcctttagtt atgtattcca gtacttgctg ctctcgtgcg gacaatcgcg cacggctggg
     9001 gctgggtgat gttgctgcca agggctggga tgtgtcttcg tggcgaaagc gcatcagtat
     9061 ctggcgggcg atcaggggac tgatcgggct gccgccctgg tgcaggctgc gaatttctga
     9121 ggcaatgctg gcagtactgc tgtctttaag cagatagccc gtcgcgccgg cctcaagcga
     9181 ctgcatgaca tgcatctcat cgccaaaagc ggtgctgacc atgatgttgc actcaggcca
     9241 tttgctatgg gcggcacgaa tgacatcgat acccgagccg tccggcaggc cgagatcgac
     9301 caacagcaca tccgccggcg cctggttgag catcttcaag ccctcagcgc gcgtgctcaa
     9361 tacggccacc agttgcatat ctgtcgcggt gctgaaggta tgccgcagtg cttgctgaaa
     9421 cgttacatcg tcctcaacca ggatcactcg aatcggcatg ctgtcatttt cgctgcgcat
     9481 cttgatcctg catgagacga gtttattgcc gcgatagtag cgcagctaat taggcggtat
     9541 tgccctaaca gagtaaggcg gcaggtttat ctgtagctat aaatggggac atacggcgcg
     9601 ggatgccttc gcttagcatc gctgtgacaa gtagggcgct tgtggcagca cgccgaacat
     9661 gaccgcccca ggccagccag tggccacgcc agcaaccata catcccaaac acactcggcc
     9721 tagttcagag gaagcaccat gtccattaag ttgcgcgctc gtcagttggg ccaaggcatg
     9781 accgagtaca tcatcatcgt ggcgctgatc gcgatctcgg ccattgtggt ttacaacctg
     9841 ttcggcagta ccgtgcgtga gcaggtcggt gacatggctg cggagctggg cggtggcgaa
     9901 gccacgcaag cggccgctac aacgggtggc gaagcgcaga cagccgccgg cgtcaaatcc
     9961 aacctgagca gtttcaagca agacgaacgc aagtaattag cagcagatat cgcggttgtg
    10021 agcgcgcacc ggcagtcggg gcaggccatg gtctttggcc tgttctttct cggcatcgcg
    10081 ctggtcggcc tgatcctgat gtacaacaac ggggtactga cccgtgatcg ggtgcaggtc
    10141 gaaaacgctg ccgacgcggc ggtttactcg caagccaagc tgtttgcccg ccaccagaac
    10201 ctgatcgcct acaccaaccg ggccatcgtc gccaacgaga tgtcggtcgg tcaggtggtg
    10261 gcgctgatgt cctggtccaa gcgctacgcc aatattccgc gctgggtgaa tacctttgcg
    10321 ccgtaccgga tccctctcgt ccctgccccc aatccgacaa tcgctgatgc gttgtccgcc
    10381 atcacgctgc cctatcagat cctcggcagt gtcacggggg tcgtggccaa gccattgctc
    10441 ggcatctatc ccgacgtgat ctccagcttc aatctggtga tgggtttctt ccagaagatt
    10501 tttgcggtcg caacgctgga ggcgcagttc accaccccaa taaacattgt tgatcgccat
    10561 gagatgccag gaaccaacga caacctgaaa gttgtcaccc tgagccattt gttgctgaca
    10621 cagaacttca tcctgacgta tttcgccagt gaactgaatt tgagcagcct ggccagccag
    10681 gcgaaaagtg ccagcacggc cggcggcggc agtgcagcct catccgaaga ctttgtcgcc
    10741 gactttctcg gcagccttgc cccctcgacc atgctggtca ataccgcgcc ggaaaagtac
    10801 gacagcagca acgcccagaa tgccgataac agtgccgcac ggacggcagg ccgtcagtac
    10861 gcggcaatca tgaacagcaa ccgcaacacc tggctggctg cgcgcaactt taatccaacc
    10921 gtcagtttga gcccaccgga actgccgatt tacctcgggt tcatcacgat aaggttgggc
    10981 ttaaacgtcg aggtgggtgt gttcaacaat ggcggcacgg cctatgtcta taaccccaag
    11041 agcacggccg ctggcggtaa aggtatcccg cagtacggct ggagctccgt cgacttcagc
    11101 tccatcggtt tcaagatgaa agtatctctg cgaatcgaat tctgcctgcc cctattaggt
    11161 tgctttgaga tactcactgg cttcccactc cagatggggc tgccacttag cggcggcacg
    11221 caccagatag tggccaaggc gggcgatcag tttctgattg gtacgcagtg gggcagcccc
    11281 atgcaaaatt caccgccagg tagctctgcg ccctacggcg aggcgatgca gtcggtgcat
    11341 gcgacgacct gggcccatgg caatgtactc ggagcctttg gtatcaacca ggctgaggac
    11401 gtcaataccg gctatggcgg cgcgccctca tttttcagcc ttggccatga ctatcaaggc
    11461 cgtggtacca gcaacgaatt taccgtggcc gtggccaagc cgctgagcag cctgcgcacc
    11521 agtgacaacc ctgccagcct gggtctggac agcggcacgt ttgccctcga gacccaggaa
    11581 gtcaaagaca acgcactgtc gtcgctgtgg ggcgggcctg accatatgat gaccatcgcc
    11641 tcggcggaat cctatttcgc cccaccgccg ggccgggtag aaacccctaa ccagttcagc
    11701 cccttctggg atgcccgcct gcgcgaaccg agccgcgtgg tgcagatgat cgcggctggg
    11761 cagattgacc tgatggccgt gctgggtctg cagccaggtg ccagcacatc ggcgattgct
    11821 ggtgcagtga tcgacagggg cctgaaaggg cttatcaacc cgggcaagga tcgggtggtc
    11881 gataagctgc cctctgttat caaagggccg gtcaagccgg tactcgacaa gttcgttgac
    11941 acgatcacaa gctcggccgg cagcgctgtc tccgagttgg tgaaatgacc atgctcgctt
    12001 ctacgggcag agcccgcatg catggccagg ccatggcgga gttcgtcgtg atggtcgcgg
    12061 gcggtgtgct cttgctgttt gtgctggtgc ccgtggtggc caaattagcg gacatggctt
    12121 acaccgccca gcaggtggcg cgttatgtgg cctgggaacg caccgtctgg tttgacactg
    12181 acaaagcgcc gggcgagacc gacgacggcg ataccgcaca acgcggcaac gaagcgatat
    12241 tgaaaactgc ggaaaaacgc ttgatggcct actcggcggc aatccagcca ttcaccgctg
    12301 ccgacatcga cggcagcgcc gacaccacca gcaataacag cctatggcgc tggacgcacg
    12361 gcggcgaagc caagccgatg acggccacgg gcagcatgcc gagccactcc agcctgaacg
    12421 cagaggacac accgtcctac agctacgacg tcatcggcat ttacaacgac gccatgggcg
    12481 gcgtgatgaa agtgctgagc tttctgaaaa tctccaagga tgacgacttt ctgcaagtcg
    12541 ctcacgagac cgagaacctt tacaacccta gcatcgtcat tcccgtttct atggccggta
    12601 gccggttagg cagcaagccg ctgtttggcg gcgcactgga caagcagttg aatattcgcg
    12661 cccagtccgc agtgcttgcc gacggctgga acgcccaggg cgatcatcat ttcaaggaac
    12721 gcgccgacga ttttgccgtg ggcaccatgc tcagcaaccc cctgatcgaa accatcatct
    12781 cgaccatcgg catgtttgaa ccctcgttca aggatgtcga gttcgactat gtcggcatca
    12841 agcccattcc ggataagccg gccaagtgca ccaccagcac agggttctgt tacttcgaat
    12901 agcctatgaa aataatcacg ccgctcttac tcagcttgtt actggccgtc aattcggcgc
    12961 aggccgagtg taactatgac gatttcccac tgatgcccga catgctggtt gccaacatcg
    13021 gcagcaacat ccagtggaac cacacgccca tggccgggcg cagttttcgc gtgccggcca
    13081 acctggcaag cgtcaaagcg ttttacgaca aggcctgggc cgacgaggtc gacttctcgg
    13141 cgttcaacgg ctgggagcag atcctgcaca tcaaccaaca gtgcatgatg ctgatccagg
    13201 taacgcgcca gaacgaccgc tacagctatg gccagctact catcaccaac cctccggaaa
    13261 aggctgcagc ggctcaagtg ctgggcgacg gtatgccgat gccgccaggc gcccaggtga
    13321 tcagtgacat gcgcagcgac gacgatatcc gccaagggcg catggtgttg ctgctcaatg
    13381 aagaggacat gcacaccacg gcccgttggt acgaaaccga gctcaaacag caaggctgga
    13441 acctggatca gcgcagccag caggccaatg gcgtggtgct cagctatagc cgtaagcgtg
    13501 agctgatgac agtcggcctg ttacgccatc aggacaagac ccagatcctg gtcaaccgga
    13561 tggatcgctg atgaaggccc aacgtggaca atccatgacc gagtacctgg tggtgctcgg
    13621 cgttaccggc gcggcgctga tgctggcggg caatgatgtc gagcgcctgt ttgatagcct
    13681 gcgcagcagc tacaaaacct actccagcga gatgaacaag gttcagctgt acaacaacga
    13741 agccgtgcgg atcctcgagc ccagccccga gccgccgttt gatgacggtc agcctgagcc
    13801 actgccgccg accgagacgg agctgcccac caacgatgcc ctcgcggtac tcaacgaggt
    13861 gtatgacgcc aacggtgtgt acgtcgggcg cgtggtgaat ggccaactgg ttgcagccaa
    13921 tggcgatgtg atcggcacct acgtccttga tgcatcgggc aacagcagcc tggttgatgc
    13981 caatggccag gttctctatc ccggcttcag catcagccag gtcttcgtcg acagcaatgg
    14041 caagccgctg gtgttgcaag cactggccaa cgccaacggt gaggtgctgg gttttgccta
    14101 tctggagggt aaaaagtact tcaatgccat caccggcaag gcgctggtca aacaacccga
    14161 agggctgacg cccatcgcca cccgtgacgt gctgatgtac gcccctgatg gcaaaacctt
    14221 cgtcgccgct tatgaagcgg cgggcctggt gtatgccaag agcgatgcgc tgatcagcag
    14281 cagtgacaca ttcaccagcc cccgcaaaat aaccggcgag ctggtcagcc tgtacctcgc
    14341 cccagagacc gaggccgcct ggagcaaata ttcactgtgc atcgtgcgta gctatggctg
    14401 ggccgccgag caggtcagca ccttgccggc aaaaggcgca agcgtggcca ttggcagcaa
    14461 tgtgtctgac cagttcaacg cccccgcccc ccaactcagt tacgtagaca gcacggcgga
    14521 tgcctgcagg gcacgcttct ccgtgctgca aaccaaggac ccggttattg ggacgcagtg
    14581 gtcgctgcgg cgcaactgat gcgtacgcgc gagccacgcc agaaaagctc taaacataga
    14641 aaaccataag agacaccgaa ccactattgc gcacctgcgc ggacagcact ttttgcactg
    14701 gggagctaca tgtcatctgt atcaggaaag aaactgctga tcatcgcagt ggcatgtggc
    14761 gtgctcgccg gggccatggg ctggctgtac ctgaaatcca aggaggccca gtaccgcgac
    14821 gcttatcgcc cgccggttga acagaaagtc acggtggtcg tgcctaaggc cgatatcggc
    14881 aaggcccagg ccttgacccc ggagctggtt gccactctgg atgtgcccag tgactacctg
    14941 gcctccaatg tcgtcctggc cgaggactgg cccaagcttg aaggccgcat gacagttgcg
    15001 ccgctgcagc gcggccggcc catcagctgg gatgccgtgg agcaggagcg cgtctcgcgc
    15061 ttctcggaga acgtcgaact gggcaagcgg atcaagacgg tcaaaatcag caagatcaat
    15121 tcctttgacg gcatgctgcg ccccggcgac cgcatcgacc tgctgggcag ttttgccgcc
    15181 ggcgatatcg gcctgcaaaa gcagcccaac tatgccgacg atgtggtgat caacatcctc
    15241 gaagacatca tcgtgctggc tgccggccgc gaagatgcca gcggccgcaa gtacgagaac
    15301 tactacgacc agagcacgcc cgacggcttc aacatgaact tttccaccct gtcgctgatg
    15361 ctcacgccgg tgcaggtggc acgggttgag ctggcggaaa aggccgggga aatggtcgcg
    15421 gttctgcgcc acccgaaaga caccagcatg gctgaactgg gccaggtcac cgtggccaac
    15481 ctgctggacc caccgcccca ggaactgatc gacgcggtgg tggatgccga cggcaacctg
    15541 attggccgca tcgtcggcga caatattgtc ggtgccgatg gccgcatcgt tggcaaagtc
    15601 gttgacgggc aagcggtagg cttcgacggc aacgtaatag gccgtactgt ccgcggcctt
    15661 aacgcggacg atcccctgct acgcatgcat gagcgcgcca cggtggtacg cgatgagcaa
    15721 ggcaatgtga tcggccgcgt ggtcgatggc aaggttatcg atgccagcgg caacgccatt
    15781 ggcaccgtcg tcgatggcaa ggccgttggc gttgatggca agtcgctggg cagggtgcaa
    15841 gccaacgccg tgctcgacag ccagggccgc gtggtcgaca tgagccgctc gcgggtgcag
    15901 gaccacgctg caccacaaaa ccaggaacgc caagtggtgc gcgatgcgac cggcaaggtg
    15961 ctcggcaggg tggacggcga caagatcgtc gatgcccagg gccaagtgat tgggcaccaa
    16021 cgtgacggca aagcctacga ccttaacggc aagccactcg gcagcatcag caccgtggtg
    16081 caagacgcgc aaggcaagat catcggtgaa atgggccaga tcgcgcgcga tgcagaaggc
    16141 aagatcatcg gtcgcgtggt cgacggccaa ttgatcggcg ccgacggcga cgtgctcagc
    16201 gtcgtcaagg acggccaggt gctcggtcag gtcgaaaaca tccagctcga cagcagcggc
    16261 cgccccgtcg agggcaacgt gcgggtggtt cgcgatgcct ccggcaaagt gctcggcacc
    16321 ctggtgggtg acgacattat cgatgcccag ggtcgtaagg tcggtagcgt caaaaatggc
    16381 gaagttcgcg atctggccgg gcaagtggtg gctggcgccc aggtggccgt tcagggcgat
    16441 gccaaggtcg tggtgcgcga tgccgagggc aacgtaatcg gcaccttgcg cggcgacacc
    16501 gtattcggcg ccgacggcaa ggccacgggg acgctgctca acggcaagct cgtcgatggc
    16561 aacggcaacg tgctggttag cggactgacg gtggccgctg aacaggccgc caacctgccc
    16621 agtgaacagt cgggcaaggc gcgcaacaag cgcctggaac cctcccgcat gatccagttc
    16681 atccctggcg gcacaggcac cgctggggtt attccggtgc agaccctgcg ccttgaataa
    16741 ccctcaccac tgcgcgcggg ctgtgctcgc gtcgactcac tcaattgcac acgctggggc
    16801 gacaatgaaa aggctacgag tgttatcgat agtgtgcggt gccgccatgg ccctgcaggt
    16861 tcaggccgac gagattcctg cagcgatggt gctgtacgac ggcgatgtgc atgtaatcct
    16921 ggcgcccggc gtggaacgcg ttgccgtcgg caatgtggcg atcatcagcg ccaccatgct
    16981 gaagaacgaa gaggtggtga tcaccgcaga aacaccgggg gaaaccacgg tgcaattgtg
    17041 gtttgccgac gaccagcgcc agcaggtcag cgtggtcgtg gccaaggcca atggctaccg
    17101 ccaggtcggc gagctgcgcg ctttgctgtc ggatattccg gggctgcgca ttcgcaccgt
    17161 gggtcgccag gtggtattgg agggacgcct gagcagtgag tacctggcgc gcgtcaagga
    17221 agccgccaag ttctatgaaa acgtgctggt gctggccgaa gagcagagcg gtgccgccac
    17281 caaggccgac tcggcccagg tgctgcaaga ggtcaaggcc atgctcgggc agattcctgg
    17341 cattcagatc aaagccgtcg gccggcagat tgtcatcgac ggcgatctgg atgccatcga
    17401 catcactcgc attgagctgc tcaaagagcg ttatccagac ctgctggtgc tggcccagcc
    17461 aatgtccaag ttcctcgcgc cgatgatcta cttcgatgtg cgcatcaccg agttcgccaa
    17521 ggacgatgtg gaagagctgg gcgtcaactg gagcaccagc atcaatggcc cgacgttcgc
    17581 cttcaacgcc gacggtggca ccaacaacct ctaccgtggg cagttcccca gtgcctccgg
    17641 caccttcgac gatctcaatg acgcggtcgg caatgtcggt tcccatgcct actggggcgt
    17701 agccagtgag atcacttcgc ggatcaacct gctggagaaa aacggctcgg ccctgaccct
    17761 ggcctcgccg cggctcagcg cccgcagtgg cggcaaggcg gagttgaccg tcggcggtca
    17821 ggtgcctgtg gtgaccagca gcatcaacgg cccctcggtg gagtacaagg acttcggcat
    17881 cctgcttaat atcgagccgc aactgtacgg tggcgaaacc atcgccacca acgtcaaggc
    17941 ggaaatcagc cagctggaca aagccaacca ggtcggtgaa tacccagcct tcaaaacccg
    18001 ccgtaccgag aacgaagtgc agctcaaagt gggggagacc ctagtactgt ccggtctggt
    18061 gaccgaagac agccaatcaa ccattgaagg catcaagtgg ttgatgaata tcccttatct
    18121 cggcggcctg ttccgcaaca aaagctataa gggcaaccgc accgaactgg tgatttttat
    18181 caccccgcgc ctgctcaccg aaggcgccga ctcgccgaac gtcaaagagc tcgcccgcga
    18241 gaaacagatg atcgagaagt acgccaaaac cgttggcaat ctcgaactga ttgattgagg
    18301 gctgagcatg tttgaagtgg tggtgaccta taaaggcggc gagcagctcg acacgctcac
    18361 ctgcacctcg gcgcaatgcg aggtgggcaa ggcgcgaacc gggctggtgc gcttacgcgg
    18421 ctggaaggtg gcccccaatc acgcgcgcat cgagcagtcg ctgggcggct tgtttgtgga
    18481 aaatatcagc cgcggcaacg agctgctggt caacggcaag ccggtggagc gcttcggccc
    18541 gcttagcccg gaagacctga tcggtatcgg tgcctataac ctgcaggtgc ggggcctggg
    18601 gcagatcgaa accgaggccg atacgccgct gccgccggat gcagcggcca gcagcggcga
    18661 ggacaacgca ccctatatgg agtggtcgca gtacgtgcag gcggagctgt tccgcgccat
    18721 ggacctgcgg cggatgaacc tcgacagcat gggcccgctg gaagtgcgca acagcctgtc
    18781 cgagctgatc aacggcatta ccgagcaaat tgcccatcgc ctgccggcca cgctcagtcg
    18841 cgagattctg cacaagatcg tgatcaacga ggcggtgggg ctgggcccgc tggaggacct
    18901 gctggccgac cccagcgtgt cggaaatcat ggtcaaccgt tacgacgata tttacgtgga
    18961 gcgctccggc cgcctggagc gcaccggcat cagcttcagc tccgactcgg ccgtgctgtc
    19021 taccatcgag cgcatcattt cgccgctggg gcggcgcatc gatgaaagct caccgatggt
    19081 cgatgcccgc ctcaaggatg gctcgcgggt caatgccatc atcccgccgc tggcgctgcg
    19141 tgggccgtgc ctgaccatcc gtaaattctc agagaaaaag ctcaccaccg acgacttgct
    19201 gggctatggc tcgatcaatg agcccatggt cgagtttctg cgtatcgccg ttgagcagcg
    19261 gatgaacatc gtggtgtctg gcggcaccgg ctcgggcaaa accacgctgc tgaatattct
    19321 ctccaacttc atcccgctgg atgagcgcgt ggtgaccatc gaagacgctg ctgagttgaa
    19381 gctggtgcaa ccccatgtgg tctcactgga agcgcgcccg gccaacatgg agggtaaggg
    19441 ccaggtgacc attcgtgacc tggtgcgcaa ctgcctgcgc atgcgcccgg atcgcatcgt
    19501 ggtcggcgag tgtcgcggcg gggaagccct ggacatgctg caggcaatga acaccggcca
    19561 cgatggttca ctgaccaccg gccacgccaa ctcgccgcgc gacatgctgc gccgtctgga
    19621 agtgatggtg ctgatggccg gcatggattt gcccgtgcag gccattcgcg agcagatctc
    19681 ctcggccgtg cagctgatcg tgcagcaaac gcgctttggc gatggctcgc ggcgtatcac
    19741 cagcatcacc gaagtcaccg gcatggagca gagcacgatt ctgctcaacg agatctttgc
    19801 ctatcaacag cagggtttcg acgagcaggg acgggtcaag ggccattacc gcgccaccgg
    19861 gcaaatcccg gagttctatg aagcgctgag caaacgcggc attggcgtga acatggacat
    19921 tttccacacc ccggagcgcc tgtaagtggc cgccgtgtgg ctggtgctgt gtgcgctgct
    19981 gctggccgtt tcggtctgcg cgctggcctt gctgatcagc cggcgggtgg ccgataacct
    20041 ggaccattac cgccggcagt tttcccgccg cgccgagctg gaaatggcag acatgttcat
    20101 tttcaccagc ggccggcaag tgctcattct caccctggtg ctgttgttgc tggtgccggt
    20161 gttgctcgaa ctgctgttcc agctgccggt ggtcacggca gctggcattg tggtgaccct
    20221 gttcaccccg cacctgatgt tcaatcacct gcgcaaggca cgcctgaaga agttcgaaga
    20281 acaacttccc gatgccttca tgctgctgtc cagcagcctg cagtccggcg ccagcctaaa
    20341 catggcgctg gagaatgtgg tgcatcaatc gccggcgccg ctgaatcagg agttcggcct
    20401 gctgatcaag aacatccgcc tcggcgtcac cctggaagaa gcgctgatca agctggaaaa
    20461 acgcctgccg ttgccctcat tcatcatggc cagttcggcc attcgcatca gccgcgaagt
    20521 gggcggcaac ctggtggaaa ccatcaacgg tatggccgcg atgctgcggc gcaagaaagt
    20581 catggagggc aagatcgaca gcctcaccgc ccagggccgc gcccagggca cctttatggc
    20641 catgctgccg atcatgctgg ccatcatcct cagctttatt gagccggaag ccatgcgcca
    20701 gctctacacc acccgcaacg gcctgatggt gctggcagtg atggtggtga tggaagtgct
    20761 gggctttatg tttatcaaaa aaatcacccg gattgacgcc tgatcatgag cgaacttctt
    20821 gtcctgttca gcagtgccag cctgggggta accgtcacct gcatcctgct cgcggtgttg
    20881 tcactgttca ccttgctgcc cgcggaaaac cgcacctaca tggacccggt gccgagctgg
    20941 attcgtccga tctggccgtt ggtgcgcctg gtcagccact tcgcctgcag caacctgccg
    21001 cccaaactca tggactggct ggacgtgcgc atgcagcgca ccggggcgct ttatgtactc
    21061 accgccgaag actttctcgc cctgtgcatc accatggcgc tgctgtttcc ggtgttggcg
    21121 atcctgccga tggccagcgg caagagcggc attctctggt cggtggtgtt gctgatgctg
    21181 ctgatgggcg ccgccttgcc cgtgatgtgg atcagcgaca cgcataaacg ccgtgaccgc
    21241 gacatcgtgc gcaacctgcc ggtgtttctc gaatacctgt ccatgtgcgt cgacgccggc
    21301 ctgaacttcc tcggcgcctt gcagcaggcc gtggataaag gccccgatgg cgccatgaaa
    21361 aacgagtttc gcattgtgct gcgcgatatc cgctccggcc tgccacgcgc cgaagcgctg
    21421 gcgcgcatgg agcaacgcat caacctgaag gatgtcacca ccttcgtacg ctcggtggtg
    21481 caggccgaga agatgggcag cagcctgcgc aacaccctgc agattcaggc ccagcagcgc
    21541 ctggaagagc gcttccagcg tgccgagaaa accgctatgc aggcacccgt gaagctgatc
    21601 attcccttga tcatgttcat cttcccgctg accttcgtga ttttgctgtt cccgattgtg
    21661 gtcaagttta tggggcaaac ctgatgcgtg aagccctggt tgtgcatacc gctggtaatc
    21721 gcccgcccag cctattgcag ctgcaacttg cccacacacc ctgggcgcgc ctgcgcggtt
    21781 tgctcggcta caaaaccctg ccggccggcc atggcctatg gctgcggccg tgtaattccg
    21841 tgcattgctg gtttatgcgt tttgccattg atgtgatcta cctggataag caacagcgcg
    21901 tgctgcaaat ccgcacacag ctgcggccct ggcaactaag cctgtgctgg ccggccaaca
    21961 gcgttatcga aatggccgcc ggtgaatgcc agcggctgaa cattacagtg ggggaccaga
    22021 tccaatgcgt ggcttaatca tcatcgccct gagcgtcgtg ttgcagggct gtgccgggct
    22081 cggactcggc agctcaaccg acctgctcga ccttgaacgc caggcgcaac aggcctacgc
    22141 ccagggcaac tacgcacgtt ccagcgagta ttacgccaag ctggcagcgc agatgcctac
    22201 cgatgccgac acccgttatc gcctggccaa tagcctggcg cgcgaaggca atcatgccct
    22261 ggcgatacgc acctatcgcg aagccttgct gcgtgacccg caacacgccg gcgcctggta
    22321 caacctgatg cagctgcaag cacaggaact gacgctgacc gctatggagg cgcgccgcta
    22381 tctggaccgg aaaaatccca gggcacaacc agccctggag cgcgccgagc aactgatcga
    22441 ggtgttcgat gcgcacgccc ccacccgcag cgaatagcca gcaggagcgt gggcaagcga
    22501 tggttgagtt cctgatcatc atcccggtgc tgatcctgct gatcttcggg gcctttcaag
    22561 cgacgctgat ctattcagca aaaagcgggc tgaactacgc ggccttccag gcggctcgcc
    22621 tgggggcggt gaataacgcg cagtacgaag gtatgcgccg tggcctgctg cgcggtatgt
    22681 acccgatgtt ctcgcagtac ccggaagcca cgcggatgga gaaaaccgcg gaggaggtcg
    22741 acaactttgt gctgatcacc cgtatcagcc cggcgctcaa cagctttact gcctggcaaa
    22801 taccccatga cgggctgcgc agcgccggca taaccgctca agccatcccc aatgacaacc
    22861 ttatgtaccg ctcggcccaa cagaacccgg tgtcgattca agatgccaat ctgctgaaaa
    22921 tacgtgtgca gtactgcgtc aaactgatcg tgccgatggt cgagaacctg ctcagcagcg
    22981 cctcgaaatt caatcagcgc caatcgggcg gcagcttcag cgaaaactcc aggcaatcat
    23041 gggatcagta cgcggccatt tgcggccagc gcaagggctt tgttctgacg tccgaggcta
    23101 ccgtgcggat gcagtcggca gccgtccatg acacggaaaa ctgcggcgcg ggcaagaaaa
    23161 tgctctgtcc ctgaaacaac cattcacgcc gcgtgagcat cttccgggct gcgcggtgat
    23221 tcgatgcgca ggtagttgtc gagtttggcc tgctgcgccg gagtggtgaa caggccaagt
    23281 ttgctgcgcc gccagagaat atcgtctgcc tgcatggccc attcttcctg gcacaggtag
    23341 tcaacttcac gggtgtacag gccgccgccg aggtgttcgc caaggtcgct gaggttgctc
    23401 acgcctttga gcatcaccca gctgcggctg ccgtagctgc tggcccagcg ccgcgccagt
    23461 gaggtgggca gccagccaaa gcgggcgcac agcgcttcca ccagcgcgct ctggctgtcc
    23521 agttgttcgg cacccggcag caggctgctg gcggtccagc ttgggcccat cttgggaaag
    23581 aacggcgcca gttgcgccag cgccgactcg gcgagcttgc ggtaggtggt gagcttgccg
    23641 ccgaacactg agagcaacgg agcttcgccg gggtggccgg acagcgacag ggtgtagtcg
    23701 cgcgtgactg ccgatggatc gtcggactcg tcatcacaca gcggacgtac gcctgcatag
    23761 gtgtgcagga tgtcgctgcg ctgaatctgc ttcttgaagt gcgcgttgac cacgtttaac
    23821 aggtagtcgg tttcttcatc gctgatcgcc accttggccg ggtcaccctg gtactcgcgg
    23881 tccgtggtgc cgatcagggt gaagcgttcc tgatagggga tggcgaagac gatgcggcgg
    23941 tcttcgttct gcagaatgta agcttgctcg ccatcgaaca ggcgcggcac gacgatatga
    24001 ctgccctgaa tcaggcggat accgtacggc gactcctgtt tcaggtcttc gcgaataaag
    24061 cgtgccaccc aggggccggc ggcattgacc aaggcgcggg cgcgaatcga gaacaagctg
    24121 ccatcgctgc gttccagatg gatatgccac aggcccttgc tgcgccgtgc gctgacgcag
    24181 cgggtgcggc tgtgcacgtg tgcgcctttt tcgcgggcgg ccatggcatt gagtaccacc
    24241 aggcgggcgt catcgaccca gcaatcggag tattcgaaac cgcgtgtgat ttcagcctgc
    24301 aatgggctgc cggcgccgaa acgcaggctg cgtgaaccgg ccagcttctc gcgcttaccc
    24361 aggtggtcat agagaaacag cccagcacga atcatccagg ccggccgcag gtgcgggcgg
    24421 tgcggcagga taaaacgcat gggtttgacg atatgcgggg ccttggtgag cagcacttca
    24481 cgttcggcta aggcttcgcg caccagacgg aattcgtagt gctccaggta acgaaggccg
    24541 ccatggatca gtttgctact ggccgatgag gtgtgttgcg ccaggtcgtc cttttcgcaa
    24601 aggaacacgg acaagccgcg cccggctgca tcagcggcga tcccgacacc attgataccg
    24661 ccgccaatca cggcaagatc atagacttca gcgagtggag tgttgctggc ggggcgagta
    24721 ggcatggcgg caccgaaagt cagtattgaa ctttgaatgt tcactttcga aaatatgcta
    24781 gtcgaataaa cacggcttcg ccaccccgaa gtgcgctttc gcgcccagtg gtcgttgatt
    24841 tttaagctga ggttattggt ttacaccagg tcgagctgaa ccttgtgttc tgccagtagg
    24901 cgattgattg ccgcggaagg tgctgcatcg gtaaacaccc ggttaaccag agcgaccgag
    24961 cccaggcgta ctaccgcatt gcgaccgaac ttgctggaat cgacggccag aaatacctga
    25021 cgcgcattgc tgataatggc ctgggagacc cttacttcct ggtaatcgaa gtcgagcagg
    25081 ctgccgtctt catcgatgcc gctgatgccg accagggcga agtcgacctt gaactgctga
    25141 ataaaatcca ccgccgcctg gccgacgatg ccgccatcgc tgcgcaccgt accgccggcc
    25201 agcagcactt cgaagtcggc cttggcgctg agctgggccg ccacatgcag gttattggtg
    25261 atgatcttca gacccttgtg gccgagcagc tcacgggcaa tggcttcggt ggtggtgcca
    25321 atgttgatga agagcgaagc gtggttggga atctgtgcgg caatggcggc agcgatgcgt
    25381 tgcttttcat cgcgcatctg cccggcgcgc atgctgtagg cggtgttctg cgtacttgag
    25441 ctttcactgg ctgcgccgcc atgggtgcga cgcagcaggc cctgttcggc gagttggttg
    25501 atgtcgcggc gaatggtttg cggggtgacg gcaaacgcct gggccaactc gtcgatgctg
    25561 acatagccgc gttcgcgggc aagttcgaga atgtcgtgtt ggcgaggcgg gagggtcatg
    25621 ggcagatcct tgaaattgcg cgccattata ggctgccgaa agtggaatag atgggctaag
    25681 gtacgccaac tttgtcagat tgctttcaca ttcgaacatg acgcctgcca ttgatctgct
    25741 gaaaaaagcc aaggctgtgc accaggtgca tagttatcac cacgatccca aggctgcttc
    25801 ctatggcctg gaagcggcgg aaaagctcaa tcttgagcca gcccaggtgt tcaagaccct
    25861 gctggcggcc agtgagaagg gcgagctgct ggtggcggtg gtgccagtag ccggtacgct
    25921 ggatctaaaa gccctggctc aggccgccgg ggtgaagaag gccgatatgg ctgacccagc
    25981 tgcagcgcaa cgggccaccg gttatctgtt aggcggtatt agcccgctag ggcagaagaa
    26041 gcgcctgcgt acttttatcg atgagtcggc gcagcagttt ccgaatatct atgtcagtgc
    26101 cgggcggcgc ggcctggaag tgcagctgga cgccgcagtg ctggcgcagc tgacgcaggc
    26161 gctgtttgca cccatcggtc gcaactgaac aacacgctct atccacataa gaacaaggaa
    26221 tcctgcatgt ctcattacct gcttgccatc gaccagggca ccaccagcag ccgggcgatt
    26281 gtcttcagtg ctcagggctt gcccgtggcg cgtgcgcagc aggagttcaa gcagtacttc
    26341 ccccaggatg gctgggttga gcatgacggc gaagagctgt ggctaagcac tctcaaagtc
    26401 tgccgtgaag cgctcaagca gagcggccta agcgccagtg atgtggcggc tatcggcatc
    26461 accaatcagc gtgaaactac cctggtgtgg gatgcgcaga ccggtgcgcc gatccacccg
    26521 gcgattgtct ggcaggatcg gcgcaccgcc gactactgcg cccagatgaa agccgctggg
    26581 catgaggcag cagtggcagc caagaccggg ctgctgatcg acccgtattt ctccgcgacc
    26641 aaattgcgct ggattctgga aaaagtcccc ggtgcccgtg agcgtgccga gcgtggtgag
    26701 ttgcgttttg gcaccgtgga ctcgttcctg ttgtggcgcc tgaccggtgg caagtcgcac
    26761 aaaaccgatg ccagcaacgc ctcgcgcacc ttgctgttca atatccacac ccagcagtgg
    26821 gatgaggagc tgctcaagct gtttgagatt cccgccagcc tgttgcccga ggtgctcgat
    26881 tgcgccgctg atttcggcat caccgaagcc aatttgctcg gtgcggccat tccggtgctg
    26941 ggaatggccg gtgatcagca ggctgcgctg attggccagg cctgctttga gccggggatg
    27001 gtcaaaagca cctacggcac cggctgcttt atgatccaga acaccggcag cgtgccggtg
    27061 atttcgaaaa atcgcctgct gaccaccgtg ggctatcgct tgaatggcaa ggtcacctat
    27121 gcggttgagg gcagcatctt cgttgccggc gccgccgtgc agtggctgcg tgacggcatc
    27181 aagctgatca gccatgcccg tgattccgag gccatggccg aacagaccgg tgacgcctgc
    27241 ggtgtgtacc tggtgccagc ctttaccggg cttggtgcgc cgtattggga cccgaaagct
    27301 cgtggcgcga tcttcggtct gacccgcgac accgggatca aggaaatcgt caccgccggc
    27361 ctgcagtcgg tgtgttacca gacccgcgac ctgctcgaag ccatgcgtca ggacggcgcg
    27421 gccgaaccta gcgcgctgcg ggtcgatggc ggcatggtgg aaaacaactg ggtgatgcag
    27481 tttctcgccg acatcctcgg ggtgccggtg gagcgcccgg aagtcactga aaccaccgcc
    27541 ctcggtgtgg cttatctggc ggggctgcag gccgggcttt ataaagacct ggcgagtatt
    27601 gcccggcatt ggcaccggca gcagcggttt gatccgcgca tggcggacag tcatcgcagc
    27661 aaactctatg acggttggct ggatgcggtg aaaagggtgc gcagcgaagg ttgatatgta
    27721 ggagccaggg ggacgcctag tccttgctgg cgatgctttc cgtcttgaga gatcaccgga
    27781 ctacagggct gcattacagc cgtagcccgg ataagccttg gcgcaatccg ggattgtctc
    27841 ggccccggat tgcgctgcgc ttatccgggc tacggggctg accttgtgct ggtgatcatc
    27901 ccttacgtgc tgttgagggc acctcaggag cctgtcagca ttaccccttc atattggttg
    27961 gtcagctcgt tcatctgcca cagcagggtt tccgtggtgg tggtgattat cgtcggcacg
    28021 tagcgtgagt cgctggccag gttgaacccg gcgcgggtaa accgccactg cgcttcggcc
    28081 tggttcaggc ccttgctgat ttgcgcagtg ttctggctgg cctgctgcag ctcctgcagc
    28141 gcgacttcaa attcgccgac ggcctgctcg aagctctgct gcagattggc gaccggcaag
    28201 cgccagctaa tggccagata cagcttggcg atacgttggc taagcatgcg ctggcgaccg
    28261 ctacggttga ccagcaccgc gctgcggcca ccgctgtggc gttcgagcag ttgcaccacc
    28321 tgctcgctct gcgccagcaa ctgatcactg agctccagca ccagcagtgc ctgtggttta
    28381 tcggggcgcg acagcgccag ttcgcggtaa cgttgccagg ttgtgccggc ctcttgcagg
    28441 gcgctgcgga tttctgcggt gggggcgtat tcgctcaggg ccaggtagtt gctttcgaat
    28501 ttggcgatgc tctggtcgag ctgctgttcg gcctgttcgg ggcggatctc ggcacccagc
    28561 atcaggtagc tcttggcaat gcgctggcta agcatgcgct gcatgcccga caggttcatc
    28621 gcttcggccg cgttaatagt ggcccaggcc gctgggctga gcagactgca gagcaggatc
    28681 agggggatga taagacgacg attcatagaa ctctccacag acacgcggcg tttgcgccgc
    28741 gcgcccatca gtagatggcc ccgttgcggg gccggtgcta tcagtagctg tactgcagct
    28801 gcgcccagag cttttcggtg tcgacggaga agtcgtcagc atcgtagttg gcgtatttga
    28861 ccaggccgac caggcccttc acgccgggga tcgggtgggc gtaggagacg tcgatttcct
    28921 cgccgtactg gctgctgtca cgctcggcgc ggaaatcgtg gtaccaggct tgcaggctgc
    28981 cgcccagcag cggtgcggta aggcctacat aggcatcctt gacgccgtcg gcgggtgtca
    29041 ggaggaagat gtcggcccag ccctggaagg cgtgtttggt cgccagtggg gtctggaagg
    29101 cgcggttgcc cgggcccgag tcaccgctca gcacttcgta gccgcccttg aaggccacgc
    29161 ccttgaagct atagcccagt tcggccaggt agtactcgct gtccagatcc tgggtgttgt
    29221 cggcgtagtc gctgaggtcg ctttgcttgg cgtattccaa ggcgtagctg acccctgcaa
    29281 tggcaccgtt gaggcgcaga ccggtggttt tgctcgactg gctgccggct gtcaggttgt
    29341 ccaggcccag acggtactgg taggcggttg cagtcagctc gggcatcacc acgtactggg
    29401 cattcagcag gtggctatgg ccttcgatat tggccgggtt agccgctgtg tcgaagcgat
    29461 tgtcaccggg gccgaagatg ctgttgacgt tgtcgatata ggcataggtc agggttaacc
    29521 cggccagcgg tttgagctga gccagggcgc cgtcgtaggt ttgctcgttc tgccgccagg
    29581 ccacgccacc gacgaaacgc tggttgtcca ggttgatgcg ctggcggccg agtaccgcgc
    29641 tgccgtactg gtggtcatag cgcagcaggg cctggttgat ctcgctgcca tccgggtcgg
    29701 caaccaccga gtagtcaccc tggccattgc gcgtgctgtt gtagctggca tcgccgatgc
    29761 ggctgacgtt atcggcctca atcagcgccg acaggccgta ccacttgccg ctctgcacgc
    29821 ctatgcgggt gcgcaaggtt tgcgcgttgg cgtgcttgag ggcgttatcc tgatcgacat
    29881 gctcatagcg gtagcgcgca tcgaaaatcg gcttggcctg gctaaacaga ttgctgaagg
    29941 actcttcggc aaaggcggcg tggctgaggg tggtgccggc aatggccagc gacagcaggg
    30001 acagtttcat catgcgcata gcggtcttcc tgtaagccaa aaaacgcaaa aaaaaacgcc
    30061 acagcgccca tgtctcaaaa ggagagggtg ctatgacgtc gttgtcggtt gagtggcaga
    30121 cacgccgttg tgctgaccgg ggtggctcat agcaggcggc gtgccaggat ttgtaagatg
    30181 ctgatttgtt aagaattagt gaatttttaa gaacctttgg cgattaaccc gtgcaccgta
    30241 gctgcccggc gcgcattcag acggtgcagc tgtaccggcg caatcgtcgg ccgctgtagt
    30301 gggcgcccgt tgccctgctg cggcatgctc tacgccacta cagataagga gagtcgcccg
    30361 atgcagcttg aatttcacca ggtcgatgct tttgccacgc agccgtttac cggcaacccg
    30421 gcgatggttt atcgcctgga caactggctt gaagatggcc tgatgcagca gattgccgcc
    30481 gagcacaacc tggcggaaac cgcttttgtg gtgaaggaag gtgcagcctg gcatatccgc
    30541 tggtttaccc ctgcagcgga agtaccgctg tgcgggcacg ctactctggc cagtgcccat
    30601 gtgctgttcg aggtctacgg cgagccgggt gatgtgcttg atttcacctg cttgtccggc
    30661 gcgctgcagg tcagccgcgc aggcgacaag ctggtgctgg attttcccgt gcgccgcgtg
    30721 cagccgtgtg aattacgtgc agacgtggaa caggcgctcg gccagccggt tgaaagtgtg
    30781 ctggcgttgc atgaagggcc gggtgttcag gagttgctgg cgattctgcc aaacgaggct
    30841 gcggtgcgga cttgtcagcc agatctggcg gcagtggctc ggttgcccgg gttgggcctg
    30901 ctgatcagcg cgccaggtga gcgccatgat ttcgtctcgc gttacttcgc ccccaccgtc
    30961 ggcatcaatg aagacccggt gaccggttcg acccactgca tgcttacgcc gtattgggcg
    31021 cagcgcctgg acaagtctca gctgagcgcc ttccagtgtt cggcgcgcgg cggcgagttg
    31081 cactgcgaat tggacggtga acgggtgaaa atcgccggcc aggcgctgtt gattgctagt
    31141 ggccggctat ttttgccgta gccgcttagc tgtcgctgct atagcggcgt actcccgact
    31201 cacccagatt gacctgggcg gcaatgctgc cgggggcggg gaataccacc agatggtcgg
    31261 cggcaacgcg aatgccgacc tgctggccgg gcaggtggtc ggcgtggctg gggaagatcg
    31321 attccaactg gctgccggtg ggcaattgca ggcgatagag ggtggcggcg ccgaggaagg
    31381 tcttgccgac gatcagggct ttttgtgtgc tgtccgggtc atagacgata tcgtccggac
    31441 gcagcagaac atccaccgcg ctgccggtcg gccaggtgta ggcgcggttg ccgcgaatta
    31501 ggcccagttc ggtctgcacc gagtcggggc taagcagctg gccgcgaatg aaatagccct
    31561 ggccgataaa gctcgcgaca aagggcgtca gcggctcgtg gtacaggttg aacggggtgt
    31621 cccactgctc cagcttgcct tccttgaaca cgccgacgtg atcgctgacg gcaaaggctt
    31681 cttcctggtc gtgggtgacc agaatcgcgc tggtgccgcg cgccttgagg atgtcgcgca
    31741 cttcatggct caagcgtcgg cgcagctcac catcgaggtt ggagaagggc tcatcaagca
    31801 gcagcagttg cggttccggc gccagagcac gggccagcgc cacacgctgc tgttggccgc
    31861 cggacagctc gtgcgggtag cgcttgccca ggtggccgag ttttaccagc tccatcaact
    31921 cttgggtgac ccgctcgcag ttgggctgtt tgcggatgcc gaagccgatg ttctcggcca
    31981 cgctcaggtg cgggaatagc gcgtaatcct ggaacaccat gccgatccgg cgtttctcgg
    32041 gggccagggt aaagccggcc ttggagataa cctgatcggc cagctgaatc tcaccttcca
    32101 gcaccggctc gaaaccggca atcgcccgta gcgtggtggt cttgccgcag ccagaagggc
    32161 cgagcaggca gccgatatcg ccggcattca ggtgcagatt gaggttctgc accaccttgt
    32221 gttcagggta gccgcagctt agttcgcgca ggttcagcag gggttggttc atgcgtggtg
    32281 atacgccggt tcaaccagaa actcgagcag cgctttctgt acatgcaggc ggttttccgc
    32341 ttggtcccag gctgccgagc gtgggtcgtc gagcagatct tcgctgattt cctcaccgcg
    32401 gtgagccggc aggcagtgca ggaacagcac gtcgtctgcg gcggcatcga gcagggcacg
    32461 gttgacctgg taaggctgga acagcttgag gcgctcggcg gcttcgtctt cctggcccat
    32521 ggaggcccac acatcggtgc tcaccagatg cgcgccagca acggcggcaa gcgggtcacg
    32581 cagaatctgt acacggtcac cagcggcggc gaggaacttg gcgttcggct catagccagc
    32641 ggggcaggcg acgcgtagct ggaagtcgaa ctgcatggcc gcttctatat aggagttgca
    32701 catgttgttg ccgtcgccga tccaggccac ggtcttgccc ttgatgctgc cgcgatgttc
    32761 ctggtaggtc tgcatgtcgg ccagcaactg gcaggggtgc aggtcatcgg acagaccgtt
    32821 gatcactgcc acgcgcgagt tggcggcgaa ctcggtcagg gtgctgtggg cgaaggtgcg
    32881 gatcatcacc gcatcgagca tgcgcgacat tacgatggcg gcgtcgccaa taggctcgcc
    32941 acggcccagc tgggtatcac gcggcgaaag aaagatggcc tggccgccga gctggatcat
    33001 cccggcttcg aacgacaggc gggtgcgggt tgaggctttc tcgaagatca tccccagcac
    33061 gcgatttttc agcggctcga acagtacgcc gcggttacgc aagtccttga gctccatacc
    33121 gcgtcggatc aggcccacca gttcatccgg tgtgcaatcc atcagcgaga gaaagtgtct
    33181 tgcgttcatc gttaactacc ttttgacagc aggggcgcag gttcaagggc cggactgaat
    33241 taaagaaaac ggcagaacct acggcttggg gccgcaccat gcgacgaaac ggggaaggcg
    33301 cgatcttata aggaaatgac gagcaggcgc aaaccacccg cggttacacc agcggtcgtc
    33361 actttgtaag ccctcgccta tatcgctgtg agagatgtca taaaaatgac ttttacggtt
    33421 gatgccgata aatcaggcac ttaggatgcc cgcgcctttt cgaggcaatg cattgccccg
    33481 caatggagta tccatggaat gttatgaaac ggagttttca tatgaagcat cttgcatcta
    33541 tcgccgctat ttccgctttc gccctgctga ccggctgtgc cagccagaat gtcagccaac
    33601 cgactctgcc gctgggcggc gcagttgaaa caggtctgaa agccgatgtg aaagtgggcg
    33661 aaatcatcac gggcgagtct tcggtgaaca ttctgttcag cgtgttcaag ctgggtggtg
    33721 atacccagtt cgccgacggc gtgacctatg gtggtggcga aggtggtttt gcgctgggtc
    33781 ttgacccggt tgcttccgct aaatctgccg ctgcatacaa ggctgtcaaa ggctcgggtg
    33841 ccgacgtgat tgttgccccg cgttacgaag taagcgtgca ggactacttc gtctacaaga
    33901 ccgtcaacgt caaagtgact ggcaataaag gcactgtcac tgcgatccgt taaggtctcg
    33961 acgctgtaat gaatcggtgg gcatgatgcc tgccgattca taagacaaag cctgctggta
    34021 tcccgttgta gctggcgcca tagttcaaat ctagcgaccc gaccggtcgt ctcgattcag
    34081 aacaagaggc caggccatga ccaagtccct ccatcaccgt gcatgccatc tctgcgaagc
    34141 catctgcggc ctgagcatcg agaccgaaac ccagcctgac ggcactgtgc agattcgctc
    34201 aatcaagggc gatgcccagg acagcttcag ccgtggccat atctgcccta aagccgtagc
    34261 gctgcaggat atccagagtg atcctgaccg cttgcgccag cccatgcgcc gcgtcggcaa
    34321 tgagtggcag gccatcgact gggatgatgc cttcgagctg gtcgccgagc gcctgagcac
    34381 gatccagacg cggcatgggc agaacgccgt ggcggtctat cagggcaacc cgagcgtgca
    34441 taactacggc ctgatgaccc acagcaatta ttttctcggc cagctgaaaa cccgtaaccg
    34501 ctattcggcc acctcggtcg atcagctgcc gcatcacctg accagccacc tgatgtacgg
    34561 ccacggcctg ctgattccga ttccggatat cgaccacacc gactttatgc tgattctcgg
    34621 cggcaacccg ctggcttcca acggcagcat catgaccgtg ccggatgtgg aaaagcgcct
    34681 gaaggccatt cagaaacgcg gcggcaaact ggtggtggtc gacccgcgcc gtagcgaaac
    34741 cgcggcgatt gccgaccaac atgtgttcgt acggcccggg caggatgccg cgctgctgtt
    34801 tggcctgctc aataccctgt ttgaagaaaa ccttggccgc gccagccacc tggcgataga
    34861 tgggctggat gaggtacgcc aggcaattgc cggctttacc gccgaaagca tgagcgccca
    34921 atgcggtgtg ccggcgcaaa cgattcgtca gttggcgcgc gactttgccg ctgctgaatc
    34981 ggcggtgtgc tatgggcgta tgggcgtctc tacccaggcc ttcggcacgc tatgtcagtg
    35041 gttggtgcag ctgatcaact tggttaccgg caacctcgac cgggttggcg gcgtgctctg
    35101 caccgagcca gccgtggacc tggtggcctc gacctccggt gggcatttca atcgttggca
    35161 gagtcgcgtg tccggcttgc cggagtacgg cggtgaattg ccggtatcgg ccctggccga
    35221 ggaaatgctc agcgagggcg aagggcaggt gcgcgcgttg gtcacggtgg ccggcaaccc
    35281 ggtgctgtcc acccccaacg gccggcagct ggagcaggcg ctggatggcc tggagtttat
    35341 gctcagtatc gacttctaca tcaacgaaac cacccgctat gccgatctga tcctgccgcc
    35401 aaccgggccg ctggaacacg atcactacga caccaccttt aatatgttcg cggtgcgcaa
    35461 tgtcacccgc ttcaatgaag cggtgctggc caagccggca ggtgcgttgc atgactggga
    35521 aatcttcatt ggtctggcca aggcctttgc cgccaagacg ggcgtggagc tcaaaccgac
    35581 catggcacct gagcagatga tcgatatggg tttgcgcttt ggtacttacg gcgacaagtc
    35641 tgaacataag ctgtctctgg cggcgctgcg tgagcatccg cacgggctcg atttgggcgc
    35701 gctcaagccc aacctgatcg gtcgtcttaa aaccgccaac aagcagattc aggcggcgcc
    35761 agcggtgatc atggccgacc tgcagcgttt tgctgaacag cccgcagcca aggcggacga
    35821 gctgctgctg attggccggc gccatgtgcg cagcaataac tcctggatgc acaactatca
    35881 ccgcctggtt aaaggcaaac cgcgtcatca gctgctgatg cacccggcgg atctgagcca
    35941 gcgtgggctg agcgatggcc aactggtgcg cgtgcgttcg cgtgttgggg tcatcgaagt
    36001 ggaagtggca gccagtgaag acatgatgac cggtgtggtc agcctgccgc acggctgggg
    36061 gcatgcacgc ccaggcgtgc agatgagcat cgccagcgaa cagccaggtg ccagcgccaa
    36121 tgacctgacc gatgagcggc aactcgacgc tttgtccggc aatgcggcgc tgaatggggt
    36181 gccggtaaag gttgaagccg cttgaccaac tgatattcgt ctcaagcaaa gtggcaggga
    36241 aggccgagca ttgcgctcgg cttttcgcta caatgccgcc accgtgccga cccgtgagtc
    36301 gggaagttaa gtcctgaggt gcccaatgga tatcatcgaa accatcaaag agcagatcgc
    36361 taaaaatacc gttctgctgt acatgaaagg ctcgccgaac gctccacagt gcggcttttc
    36421 ctccaaagcg gcgcaggttg tgatggcctg tggcgagaaa ttcgcttacg tcgacatcct
    36481 gcaaaacccg gaaatccgcg ccaacctgcc aaagtacgcc aactggccaa ccttcccaca
    36541 gctctgggtt ggtggtgagc tggtcggcgg cagcgacatc cttgcggaaa tgtttgaaaa
    36601 gggtgagctg cagaccctga tcaaggacgc tgtgcagaag gccgacgctt aatccgcagc
    36661 acataaacaa caaaccccgc caagtgcggg gtttgttgtt tatggcgcaa gcgttctggc
    36721 ttattcgtcc atctgcgatt gcagatagtt ctgcaggcct acggccgcga tcaggctgag
    36781 ctgggtttcc agccagtcga tatggtcttc ttcggagcag aggatttctt caagcagttc
    36841 gcggctggcg tagtcaccga tgctttcgca gtaggcaatc gccgctttca ggtcgatatg
    36901 tgctttgtgc tcaatcttca ggtcgcactc aagcatttcc tgggtgttct caccgatcag
    36961 gatcttgccg aggtcttgca cattgggtag gccttcgaga aacaggatgc gcttgatcag
    37021 cttgtctgca tgcttcatct cgtcgatgga ctcgtggtac tcgtgctcac cgagcttctt
    37081 caggccccag tcctcataca tgcgtgcgtg caggaagtac tggttgatcg cgaccagctc
    37141 gttaccgagg atcttgttca gatgctggat gaccttcttg tcgcctttca tgtctgcgtc
    37201 tgccttgcag gggagttagc gctaaattct gggccgatgg ttttcggctg tcaaacctaa
    37261 gtatttgaat ttaaagttaa aattaatatg aatacgaatg cttaagttct gcgctttggt
    37321 gctaacacat tgaatttcag gcataaaaaa tgtctgggag acatttttaa agttatgtag
    37381 cgaaggcctg cagggcggct ggccataaaa aaaccggacg ctaggtccgg ttcttgtata
    37441 tcgctaaatt cacgcggcaa caaaactcgc cgggtaggcc atggcggctt ggccgttctg
    37501 cacgtcgctc aaggtctcgc gtaccacttg cttggccagg caggcgcatt tgccgcattg
    37561 gctggcaacg ccgaggctgg cgcggacatc gcgatagctg cagcagccat cgtagatggc
    37621 atcgcgaatt tgaccgtcag taacaccttc gcaaagacag acgtacatga ttta
//
LOCUS       DASRUM010000143        14757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1738228, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000143 DASRUM010000000
VERSION     DASRUM010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 14757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14757
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738228"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..1118)
                     /locus_tag="VFF22_09550"
     CDS             complement(333..1118)
                     /locus_tag="VFF22_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17091.1"
                     /translation="MNRITFAAALCGLLMVCSARADDCQQHIPALLEQAYPGHAQGQP
                     LEDERGAYRIASELSVCKVWPARPHLTLLALALIRAEDEGYGESDLQVLVLDTASQAI
                     VARHVAPNLLDWDAIFVDGLEFDTAPYRLRDETLAFGVRVKRRGSSRANPLSETYLSL
                     YELEAQHLRPVLSGLRIDRSWGEWDTRCAGEFSDTQGLLIITEQRGNEGYRDLLLKQR
                     RVESRSAEVAEACETVDESTHRAQFRIEYDEQHYLLPIEFSPI"
     gene            complement(1263..1568)
                     /locus_tag="VFF22_09555"
     CDS             complement(1263..1568)
                     /locus_tag="VFF22_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17092.1"
                     /translation="MMRLLMQFSACSALGLAVAFMVLLGLMFCGQFDLIKGLQLTGQP
                     LAQLSLVLLPEAFWESLTGLANATQNREVQSFLSLCTSLGQVGLLLAFGFFRLWYVR"
     gene            complement(1625..2983)
                     /gene="creD"
                     /locus_tag="VFF22_09560"
     CDS             complement(1625..2983)
                     /gene="creD"
                     /locus_tag="VFF22_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HZX17093.1"
                     /translation="MSRILGFKLGAITLLILLLLVPLSMIGGLVSERQYQRAEVLQDI
                     ARSSSYRQQLTGPILVMPYTKVWHVWKTHTKTGERYTEEQRSRGRLYFLPERFVLNGQ
                     VATEERARGIYKALLYRSDNQISGAFKLPARLGLGDDLGLYQFDRPFLSVGISDIRGI
                     SNDLQLRFNGMNLSFAPGSAEERFGAGVHAPLPVLDSQGGQTLEFAFDLKLQGTEQLS
                     ITPVGRDSLVKLTSGWPHPSFVGEYLPSSREVSDKGFMAQWQTSFFATNLEEALADCV
                     GQDSCTALTARNFGVSFVDPVDQYLKTERAIKYALLFIGLTFAVFFLFEVLKRMAVHP
                     VQYALVGMSLALFYLLLLSLSEHLSFALAYGIAATACVLLIGFYVSYVLHSALRGAGF
                     AALLALLYGMLFGLLGAEDYALLMGSVLVFGVLAGVMVLTRKLDWYGVGRLPLANPAS
                     DR"
     gene            3159..3599
                     /locus_tag="VFF22_09565"
     CDS             3159..3599
                     /locus_tag="VFF22_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003302952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HZX17094.1"
                     /translation="MTQHNPYATPQASVVDAESLVSDEQIAALPISDKWKQRFSAIHH
                     AGGIKMPKFKQLPAEERRKAFSFNFLAFFFGPIYYAIKGMWKKGLALFLVCAVVVIIL
                     GIGLDYLGYSKIANALGYGISAVFATRANIDYYKKMLLNDNGWW"
     gene            3775..4245
                     /locus_tag="VFF22_09570"
     CDS             3775..4245
                     /locus_tag="VFF22_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZX17095.1"
                     /translation="MTEYVAEVIWQRGEQDFLGNRYSRKHLLRFDGGLEVAGSSSPHV
                     VPLPMSDASAVDPEEAFVASLASCHMLWFLSIAAKRRFCVDDYRDNASGLMQANAAGK
                     LFIAQVTLRPAVSFSGERMPSREQIEQMHHQAHEECFIANSVKSEVRCEPRFSE"
     gene            4318..5154
                     /locus_tag="VFF22_09575"
     CDS             4318..5154
                     /locus_tag="VFF22_09575"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX17096.1"
                     /translation="MNPTAYGCPDGQPLFYFHGVPGAAVEAAMLHQAAFAHGIKLLVV
                     ERDLPGELSSAAHLQQLAQQISDRAEDQPIRLLGFSIGACLALRVAALLGERVAAVYL
                     LSAAAPLEIPSNSVGMGAGRYTFLWAQHYPRLFRVFAWYQALLAKVSPRLLLKLLFSG
                     AGGKDPELLHQADTQAWLADIQRACFKHGTNSYVRDVKLYVAPWAAELDKVTAAVSLW
                     HGEADNWAPIAMSIYLQEHLRTTSAVTRCAEQAHYSCLLENAQAVMAAVQLNISATRA
                     AE"
     gene            5207..5548
                     /locus_tag="VFF22_09580"
     CDS             5207..5548
                     /locus_tag="VFF22_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008026584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HZX17097.1"
                     /translation="MKKLALPLLTLLAFSAYTLYVMLHAQQSLLQFGMQLMSSADTAQ
                     VVIDLYILAALACIWMYRDGRARGKSLAYLLPFFALTALFVSIGPLLYLVLKALDTGS
                     KAPQDSASTAA"
     gene            complement(5589..5741)
                     /locus_tag="VFF22_09585"
     CDS             complement(5589..5741)
                     /locus_tag="VFF22_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HZX17098.1"
                     /translation="MTLALTPLISLIAGILILVMPRLLNYIVAIYLIVIGLVGIFGTG
                     NFNLSF"
     gene            5862..6371
                     /locus_tag="VFF22_09590"
     CDS             5862..6371
                     /locus_tag="VFF22_09590"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZX17099.1"
                     /translation="MNNASLLTSLLQYKAWANQELFAELQRLDPLTQHSELHAALRIL
                     NHIHVVERIFVANLQGISHDYSATNTSETPTLAALQQAVQDTDRWYLDYAATLSAEQL
                     AERRRFKFVDGDTGCMSREEMLAHIVTHGGYHRGAVGRIMAQVQLAPPRDIYTRFLHQ
                     DQPQRRQAS"
     gene            complement(6387..6824)
                     /locus_tag="VFF22_09595"
     CDS             complement(6387..6824)
                     /locus_tag="VFF22_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003152136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZX17100.1"
                     /translation="MKTVAEILRAKPQAAVYSVTPSTSVLDAIKLMAEKGIGALVVLD
                     DKGRLAGIVSERDYARKVALLERSAFSAMISEIMTADVLTVGLRDTSQHCMQLMTDRH
                     LRHLPVLAEGELIGLLSIGDLVKHTIAEQASLIQQLEQYIRGE"
     gene            complement(6970..8403)
                     /gene="creC"
                     /locus_tag="VFF22_09600"
     CDS             complement(6970..8403)
                     /gene="creC"
                     /locus_tag="VFF22_09600"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HZX17101.1"
                     /translation="MPLGIRIFLVYFLFVGLAGWFVLSTVMDEVRPGVRQSTEETLVD
                     TANLLAEILRDEVKAGRLQQSRLPELLQAYGQRQPQAQIWGLEKNQVNHRIYVTDAKG
                     IVLLDSTGAAVGQDYSRWNDVLRTLRGEYGARSTKEDPNDPDSSVMYVAAPIMDGAQI
                     IGVVSVAKPNRTLQPYIERSEQRLAWLGGGLIVLGLLIGALLSWWLSAALRKLTRYAQ
                     AVSAGQRAELPSVRGGELAQLAEAIERMRTELEGKAYVERYVHTLTHELKSPLAAIRG
                     AAELLDGEMPAAQRQRFVANIEQESARMQQLIERLLHLAQVEQRQGLEEQVAVPLQAL
                     VDSLLQAQMARIEAAGLQIENHIGGELSACGESFLLRQALANLLDNALDFTPPGGLIR
                     FNAQALGGQIELRLFNQGEAIPDYALARLSERFYSLPRPGSGRKSTGLGLNFVEEVMQ
                     LHGGELHIGNVAGGVEAQLRLPLFPGS"
     gene            complement(8403..9089)
                     /gene="creB"
                     /locus_tag="VFF22_09605"
     CDS             complement(8403..9089)
                     /gene="creB"
                     /locus_tag="VFF22_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HZX17102.1"
                     /translation="MPHILIVEDEAAIADTLIFALQSEGFTTTWLSLAESALAEQQRN
                     PADLLILDVGLPDISGFEACKRLRRFSEVPVIFLTARSEEIDRVVGLEIGADDYVVKP
                     FSPREVAARVKAILKRMAPREAPATNSDSGPFQLDIERVQIHYHGQLLSLTRHEFRLL
                     QSLLAQPERVFSREQLLDGLGVAADVGYERTIDSHIKSLRAKLRQVAAAAEPIQTHRG
                     LGYSYSPRHA"
     gene            complement(9248..11278)
                     /locus_tag="VFF22_09610"
     CDS             complement(9248..11278)
                     /locus_tag="VFF22_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX17103.1"
                     /translation="MTGVLKPGVRLLERFSFARKFQLVFVLFVLPLAYALWVISSDHL
                     GRLALIDNELEGMRGVQSIGAVQRAVLEQRTLSARWKGTDTAAEALLKEREAALDDAL
                     TAAIEQLQDDMLSPQTQAHLQSLQTLRAELAVGAMAKVPLPEALERYQKALLQVMTLR
                     EQVATDSGLILDPYLDSYLMMEQLTFTLPRLLDNLGGFVSQGYGAVFSQHFTLQSRVL
                     MRDLRRALDESRSQLIKARTTLAQEAPRVMRNLDAPFAAAEQGLDQFLSDIDRDMFQA
                     SPMTLTPAQFIERVAVLEGHLRALQDAQYRQFAETLGHYRSEAQWKMTEIILAFGVLT
                     LIALYVLLCLNASIRRSTASIISAAEGLRDGDLRVRMEVHGEDDLAAIARALNISVGQ
                     LRSSLQGVDLESQRLGGTVQSLSHEAQTALAAVEQQQAQMSQIAAAATEMAATAQSVA
                     QSCEQAAVEAGQTREIAEQSNQRSARTSASMRQLSSRLGDSATTLQQLRTQTEQITRV
                     VDVIKGIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRSLSQRTQNSTAEIAETVA
                     NLHRVVGQSVTQMEDAVRQAEGDVGSVLAMGEDLNAIVESVQLVTDRLAQIATAAEQQ
                     AATADEVSGNIQQVDHAASALLEGAQAVSNAAEQLRQGSQALEQNTARFKLV"
     gene            complement(11452..12678)
                     /locus_tag="VFF22_09615"
     CDS             complement(11452..12678)
                     /locus_tag="VFF22_09615"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HZX17104.1"
                     /translation="MLKCIAVADVRVGMYIHEFCGAWMDHPFWKSKFLLSSENDLLRI
                     KASSIGELWIDVSKGLDVAVGTASVSPEEVAAEAEAALLAAVQSPPVNLAVSMDDEVR
                     RAAKLCERSKAAVVSMFGDARMGQALQFEQAGELVEEISDSIMRHPNALISLARLKNA
                     DEYTYMHSVAVCALMIALARNLGLNEGQVREAGLAGLLHDIGKMAIPSEVLNKPGKLT
                     DNEFATVRDHPEVGSRMLLDSKQVSALVLDVCLHHHEKMDGTGYPHRLEGEQISLYAR
                     MGAVCDVYDAITSNRPYKQGWDPAESIRKMAEWKGHFDPVVFQAFVKTVGIYPVGSLV
                     RLESGRIGVVMEQQAKSLLAPRVKVFFSAKSKTPIPQEILDLSKLVGRDKIVGRESAE
                     EWGFRNVEELWSGISR"
     gene            12860..13753
                     /locus_tag="VFF22_09620"
     CDS             12860..13753
                     /locus_tag="VFF22_09620"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZX17105.1"
                     /translation="MRRLLTGLSVIILLLLNTLILIGPLLLIALGKFILPGKALKTAS
                     SRGVMWVAETWAELNKLIFATLLPVHWDIRGNADLRQSTSYLVISNHQSWVDIPALVQ
                     AFNRKTPYFKFFLKQQLIWVPFLGVAFWALDYPFMKRYSKAFLAKHPELKGQDLEITK
                     LACEKFKDLPVTVVNYLEGTRCTPAKQAQQGSPYQYLLKPKAGGVAFVLAALGEQLDT
                     VLDVTVVYPGARIPGFWDLISGQVDKVIVDIQTRDLDPALWQGDYENDPVFRQFVQDW
                     VTKMWQDKDARIAAIHAEIEV"
     gene            complement(13842..>14757)
                     /locus_tag="VFF22_09625"
     CDS             complement(13842..>14757)
                     /locus_tag="VFF22_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZX17106.1"
                     /translation="VACGRCRSCKEQHTGVCLSVNPARAGGAYGYVDMGDWTGGQAEY
                     AMVPYADFNLLKLPDRDKAMEKIRDLTCLSDILPTGYHGAVTAGVGPGSTVYIAGAGP
                     VGLAAAASARLLGAAVVIVGDVNPVRLAHAKAVGFEIADLSKDTPLHEQIAALLGEPE
                     VDCAVDAVGFEARGHGHAGVQHEAPATVLNSLMGVVRVAGKIGIPGLYVTEDPGAVDA
                     AAKIGSLSIRFGLGWAKSHSFHTGQTPVMKYNRALMQAIMWDRINIAEVVGVQVISLD
                     DAPRGYGEFDAGVPKKFVIDPHKMFSAA"
BASE COUNT         3010 a         4645 c         4311 g         2791 t
ORIGIN      
        1 aatcagccga acattcgcct tccagtacgc tgcagcgtta gcttgatcag aagtcataaa
       61 ctcctccggt tattgttatt ctcatgcacc ttcaagctag cagcaggcca ctagggccga
      121 cagatagaca ttggtattag ccgtcttaaa ccaccataag cgcagcgctt tttgtcgatt
      181 taacagtcac ttggcactac accggaaagc ccccaaaaaa gcggaaacaa ctttggtcta
      241 agagtaaggc gggaacatgc ctgttgcggg cgcgcaatca gctgcagagt gcccacccct
      301 gctgtgcaat atcgaagctg tttagcgccc ggctagatag gggaaaattc gatcggtagc
      361 agataatgct gctcgtcgta ttcgatgcga aattgcgcgc gatgggtgct ttcgtccacg
      421 gtttcgcagg cctcggctac ctcagcgcta cggctttcga cccgacgctg cttgagcagc
      481 aggtcgcggt agccctcatt tccgcgctgc tcggtaatga tcaacagccc ttgggtatca
      541 ctgaactcgc ccgcacagcg ggtatcccat tcgccccacg agcggtctat gcgcaacccg
      601 ctcaacacgg ggcgcaaatg ctgcgcttcc agctcgtaaa ggctcagata ggtttcagag
      661 agggggttgg cccgcgagct gccacggcgc ttgacccgca cgccgaaggc cagcgtctcg
      721 tcacgcaggc gatagggcgc ggtatcgaat tccaatccat cgacaaatat cgcatcccaa
      781 tccagcaggt tcggcgcaac atggcgcgcg actatggcct ggctggcagt atccagcacc
      841 agcacttgca agtcgctctc gccataacct tcatcctcgg cccgtatcag cgccagggct
      901 agcagtgtca ggtgcggacg ggccggccag accttgcaga cactcagttc gctagcaatg
      961 cgataggcgc cgcgctcgtc ctccaggggc tgaccctggg catggcctgg gtatgcctgc
     1021 tcaagcaatg caggtatgtg ttgttgacag tcatcggctc gcgccgagca caccattaac
     1081 aggccacaca gtgctgcggc gaaagttatg cggttcatcc ctgagctcct tctgataatc
     1141 cagcggccag tctaaacccg acgcggcaca cggcccatgc tgggcgtcac ggaataaccg
     1201 cgcaatccgc cgcgccagca aatgcccggc gcgcgactct tcgcgtaacc cgatatgccc
     1261 gctcatcgca cataccacag acgaaagaag ccaaacgcca gcagcaggcc gacttggcca
     1321 agcgacgtgc acaggctgag aaaggactgc acctcacggt tctgcgtggc attggccagg
     1381 ccagtcaggc tctcccagaa tgcttctggc aacaacacca ggcttaattg cgccaggggt
     1441 tggccggtca gctgcaggcc tttgatcagg tcaaactggc cgcagaacat aaggcccagc
     1501 aacaccataa aggccaccgc cagccctaaa gcagaacagg ccgagaactg catcagtaaa
     1561 cgcatcatgt ttatctcccg cacagataaa aaagcggcgc aggccgggcc tgcgccgcac
     1621 cgtatcaacg gtcgctggct gggttggcca gcggcaggcg gccgacgccg taccagtcga
     1681 gtttgcgggt cagcaccatc accccggcga gcacgccgaa caccagcacc gagcccatca
     1741 gcagggcgta gtcctcggcc ccgagcaagc cgaacaacat gccgtagagc agcgccagca
     1801 aggcggcaaa gcccgcgccg cgcagagcgc tatgcaacac gtaactgacg tagaagccga
     1861 tcaacagcac gcaggcggtc gccgcaatgc cataggccag ggcaaaactc aggtgctcgg
     1921 acagcgacag cagcaacagg tagaacagcg ccagcgacat gcccaccagg gcgtattgca
     1981 ccgggtgcac ggccatgcgt ttgagcactt caaagaggaa gaacacggcg aaggtcaggc
     2041 cgataaacaa cagggcatat ttgatcgccc gctcggtctt caggtactgg tccaccggat
     2101 cgacaaaact cacaccgaag ttgcgtgcgg tcagtgcggt gcagctgtcc tggccaacgc
     2161 aatcagccag cgcctcttcc aggttggtgg caaagaagct ggtctgccac tgcgccataa
     2221 agcccttgtc cgacacctca cggctgctcg gcaggtattc gccgacaaag ctcggatgcg
     2281 gccagccgga cgtcagcttg accaggctgt cgcgccccac cggggtgatg ctcaactgtt
     2341 cggtgccctg cagtttgagg tcgaaggcga actccagagt ttgcccaccc tggctatcca
     2401 acaccggcag cggcgcatgc acaccggcac cgaaacgctc ttcggcgctg ccaggggcaa
     2461 agctcaggtt catgccattg aagcgcagct gcaggtcgtt gctaatgccg cggatatcgc
     2521 tgatgccgac cgagagaaat ggccggtcga actggtacag gcccagatca tcgcccagcc
     2581 ccaggcgcgc cggcagcttg aatgcaccac tgatctggtt gtcgctgcgg tacagcagcg
     2641 ccttgtagat gccacgcgca cgctcctcgg tggcgacctg accattgagc acaaagcgct
     2701 ccggcaggaa atacagacgg ccacggctgc gttgctcctc tgtataacgc tcaccggtct
     2761 tggtgtgggt tttccacacg tgccagacct tggtgtacgg catcaccaga atcggcccgg
     2821 tgagctgctg gcgatagctg gaactgcgcg cgatgtcctg cagcacttcg gcgcgctgat
     2881 actgccgctc gctcaccagc ccgccaatca tcgacagcgg gaccagcaat aacaaaatca
     2941 gcagggtaat agcgcccagc ttgaaaccca ggatgcggct catggtgcaa ctctccgata
     3001 cggtttaggt aggcagagtc tgggctgcgc aggtggtggc tttatggagg gtagatggag
     3061 agtgtgtgga gagtccgtac aagcagccaa tcgacacata gacagccata tgggaaatgc
     3121 gtagcgtgcc gctgatcgcc cattaaggat gacaggccat gacccagcac aacccctacg
     3181 ccaccccgca agccagcgtc gtcgacgcag aatcactggt gtccgacgag caaatcgccg
     3241 cactacccat ctcggataaa tggaagcagc gctttagcgc gatccatcat gccggcggca
     3301 tcaagatgcc caagttcaag cagctgcccg ccgaggagcg gcgcaaggcc ttcagcttca
     3361 actttctggc gttcttcttc gggccgatct actacgccat caagggcatg tggaaaaagg
     3421 gcctggcact gtttctggtc tgcgcagtgg tggtgatcat cctcggcatc ggcctggact
     3481 acctcggtta cagcaaaatc gccaacgccc tgggctacgg catcagcgcg gtattcgcca
     3541 cgcgcgccaa tatcgattac tacaagaaga tgctgctgaa cgataacggc tggtggtaag
     3601 cgtccaacaa gctgttggaa aactacctgc gttgccagcg cggcgttaaa aacaggctca
     3661 aaatgctcat ttacagctcg taaactccgc tttttcgcct gtttttgcct tgcgctggct
     3721 gcctcgccta cgtttttcaa cagcctgtta acccaactgc atcaggagaa tgacgtgacg
     3781 gaatatgtcg ctgaggtgat ctggcagcgc ggcgagcagg actttctcgg caaccgctat
     3841 agccgcaagc atctgctgcg ttttgatggc ggcctggagg tcgccggctc gtcctcaccc
     3901 catgtggtgc cgctgccgat gtcggatgca agcgccgtcg atcctgagga ggcctttgtc
     3961 gcctcactcg ccagctgcca tatgctgtgg tttctatcca tcgccgccaa gcgtcgcttc
     4021 tgcgtcgatg actaccgcga caatgccagt gggctgatgc aggcgaatgc tgccggcaag
     4081 ctgtttatcg cccaggttac cttgcgcccc gctgtcagct tctctggtga gcgcatgccc
     4141 agccgcgaac agattgagca gatgcaccac caggcccacg aggagtgctt tatcgccaat
     4201 tcggtgaagt ccgaagtgcg ctgcgaaccg cggtttagcg agtaacgcca caaggccaca
     4261 tcgattactg cgacaagatg tgctttaacc atagcggctg gtggtaggag aagatgcgtg
     4321 aacccaactg catacggctg ccccgatggg cagccattgt tctacttcca tggcgtgcca
     4381 ggcgcggcgg ttgaggcggc gatgctgcat caggccgcct ttgcgcatgg catcaagctg
     4441 ctggtggtgg agcgcgatct gccgggcgaa cttagtagcg cggctcatct acaacagctc
     4501 gcgcaacaga tcagtgatcg cgcggaggac cagccgattc gcctgcttgg tttttccatc
     4561 ggcgcctgcc tggcgttgcg agtcgccgca ctattgggtg agcgggtggc tgcggtgtac
     4621 ctgctctccg ccgccgcgcc gttggaaatc cccagcaata gcgtcggcat gggcgcgggg
     4681 cggtatacct tcctttgggc gcagcattat ccaaggctgt tccgggtctt tgcctggtat
     4741 caggcgctgc tggcgaaagt cagcccgcgc ctgctgctga agctgctgtt cagcggtgct
     4801 ggcggtaaag acccagaact cctgcatcag gccgacaccc aggcatggct ggcagatatt
     4861 cagcgcgcgt gtttcaagca tgggactaac agctatgtgc gcgacgtaaa actctacgtc
     4921 gcgccctggg ctgctgagtt ggataaggtc acagcggcgg tcagcctgtg gcatggcgag
     4981 gcagataact gggcgcccat tgccatgtct atttatctgc aggagcatct gcgaacgacc
     5041 agcgcggtga cccgttgcgc cgaacaagcg cattactcct gcctgctgga gaacgctcaa
     5101 gccgtgatgg cggcggtgca gctgaacatt tcagcaacgc gagcggccga gtgataacgc
     5161 catagagtgc agacgcttcg acttaaaacg acccaaggac gacaccatga aaaagctcgc
     5221 actgccgctg ctcaccctgc tggccttcag cgcctacacc ctgtacgtga tgctgcatgc
     5281 gcagcaatcg cttctgcaat tcggtatgca attgatgtcc agcgccgaca ccgcccaagt
     5341 ggtgatcgac ctgtatatcc tcgcggccct ggcctgcatc tggatgtacc gcgacggccg
     5401 cgcgcggggc aaatcgctgg cctacctgct gccattcttt gccctgactg cgctgtttgt
     5461 ctcgatcggg ccgctgcttt atctggtgct caaggccctc gatacaggta gtaaagcgcc
     5521 acaggatagc gcctcaactg cagcctgatg ggacggagca gccccggaaa accggggctg
     5581 ctcaacggct aaaagctcag attgaaattg ccggtgccga agataccgac gagaccaatc
     5641 acgatcagat aaatcgccac gatgtagttc agcaaacgcg gcatcaccag aatcagaata
     5701 ccggcgatca gcgaaatcag tggagtaagg gcaagggtca taatcgtcac ctgagagtta
     5761 ttgaagcgcc agtatgcgcg caacgcccaa cgcgcacagt acgatccacc acagagtcgc
     5821 cctgtatccc tgtaaagcaa tccgcgtatt gaaggccact tatgaacaac gccagcttgt
     5881 taacctcatt actgcagtac aaggcctggg ccaatcagga actctttgct gagttgcagc
     5941 gactcgaccc gctcacccag cacagcgaat tgcacgccgc cctgcgcatc ctcaaccata
     6001 tccacgtggt cgagcggatt tttgttgcca acctgcaagg cattagtcac gactacagcg
     6061 ccaccaacac ctcggaaaca cccacactgg cagcgctgca gcaggcggtg caagacactg
     6121 atcgttggta tctggactat gccgcgacgc taagcgccga gcagttggcc gagcgccggc
     6181 gcttcaagtt cgtcgatggt gataccggct gcatgagccg cgaggaaatg ctcgcgcata
     6241 tcgtcaccca cggcggttac caccgcggcg ccgtcggccg gatcatggcg caagtgcagc
     6301 tggcaccacc cagggatatc tacacacgct ttctgcatca ggatcagccg cagcgccgcc
     6361 aagcgagcta gcagtattta cgcgaactac tcgccgcgaa tgtactgttc cagctgctgg
     6421 atcaggctgg cctgttcggc gatggtgtgt ttgaccaggt caccaatcga cagcaggccg
     6481 atcaattcgc cttcggccaa tacgggtagg tgacgcagat ggcggtcggt catcagttgc
     6541 atgcagtgct gcgaggtgtc acgcaggccg acggtcagga catctgctgt cataatttcg
     6601 ctgatcatcg cactgaatgc ggagcgctcc agcagcgcca ctttgcgcgc gtagtcacgc
     6661 tcgctgacga tgccggccag gcggcctttg tcatccagca ccaccagtgc accaatgcct
     6721 ttctcggcca tcagcttgat tgcatccagc accgaggtac tgggggtgac gctgtacacg
     6781 gcggcctggg gcttggcgcg aaggatctcg gcaactgttt tcatgcagtg gctcctgctc
     6841 tgtttcttat tggcattgag gatagtgcgc gggaaggccg ttgcgggcca acccgatgac
     6901 ccgatcagag cacctgtgcg ggctcagcgc aaccattgca agccggcaag gtcggtgcca
     6961 ggccgcgcgt cagctgccag ggaacagcgg cagacgcagc tgcgcctcta ccccgcccgc
     7021 gacattgccg atatgcagtt cgccgccgtg cagctgcatc acttcctcga caaaattcag
     7081 ccccaaaccg gtgcttttgc gaccgctgcc ggggcgtggc agggagtaga agcgctcgct
     7141 caaacgcgcc agggcgtaat cggggatcgc ctcgccctgg ttaaacaggc gcagctcaat
     7201 ctgtccgcca agggcctggg cgttaaagcg gatcagcccg cctggagggg tgaagtccag
     7261 cgcgttatcc agcagattgg ccaacgcctg acgcagcaga aaagactcac cgcacgcgct
     7321 taattcaccg ccaatatggt tctcaatctg caggccggcc gcttcaatgc gcgccatctg
     7381 cgcctgtagc agggaatcga ccagtgcctg cagcggcact gccacctgct cttccaggcc
     7441 ctgacgctgc tcgacctggg ccaggtgcag caggcgctcg atcagttgct gcatgcgcgc
     7501 gctttcctgc tcgatattgg cgacgaaacg ctgccgctgc gccgccggca tctcgccatc
     7561 gagcagttcc gccgcaccgc gaatcgccgc cagtgggctt ttcagttcgt gggtcagggt
     7621 gtgtacgtaa cgctcgacat aggccttgcc ttccagctcg gtgcgcatgc gttcgatggc
     7681 ttcagccaac tgtgccagct cgccaccgcg cacgcttggc agttcggcac gctgcccggc
     7741 gctaacggcc tgggcgtagc gggtcagttt tcgcagcgcg gcgctaagcc accaggacag
     7801 taacgcgccg atcaacaggc ccagcacaat caagccaccg ccgagccagg ccaggcgctg
     7861 ctcggagcgt tcgatatagg gctgcaaggt gcgattgggc ttggccaccg agaccacgcc
     7921 gataatctgc gcgccgtcca tgatcggcgc ggccacgtac atcaccgagg agtcgggatc
     7981 gttcgggtct tccttggttg agcgcgcgcc gtactcgccg cgcagcgtgc gcagcacatc
     8041 gttccagcgc gaataatcct gacccaccgc tgcgcccgtg gagtcgagca acacaatgcc
     8101 cttggcgtcg gtgacataaa tgcggtggtt gacctggttt ttttccaagc cccagatctg
     8161 cgcctgcggc tggcgctggc cgtaagcttg caacagttcg ggcaggcgac tctgttgcag
     8221 gcggccggcc ttgacctcgt cacgcagaat ttccgccagc aggttggcgg tatccaccag
     8281 ggtttcctcg gtggactggc gcacgccagg gcggacttcg tccatcaccg tgctcagcac
     8341 aaaccagccg gccaggccga caaacaggaa gtacaccagg aagatgcgta tccccagcgg
     8401 catcaggcgt gtctcggtga atagctataa cccaggccgc gatgagtctg gatcggctca
     8461 gccgcagcgg ccacctgacg cagcttggcg cgcaggcttt tgatatggct gtcgatggtg
     8521 cgctcgtagc cgacatcggc ggcaacgccc aggccgtcaa gcaactgctc gcgggagaac
     8581 acccgctcgg gctgggccag caggctttgc agcaaacgga attcgtgacg ggtaaggctg
     8641 agtagctgac cgtggtagtg gatctgcacc cgctcgatat ccagctggaa cgggccactg
     8701 tcggagttgg tggccggcgc ttcacgcggt gccatgcgtt tgaggatggc cttgacccgc
     8761 gcagccactt cccgcgggct aaagggtttg accacataat catcggcgcc gatctccagg
     8821 cccaccacgc ggtcgatttc ctcgctgcgt gccgtcagga aaatcaccgg cacctcggag
     8881 aaccgccgca aacgcttaca ggcctcgaag ccgctgatat ccggcagacc cacatcgaga
     8941 atcaacaaat cagccggatt gcgctgctgc tcggccagtg ccgactcggc caggcttagc
     9001 caggtggtgg taaagccttc gctctgcaga gcgaagatca gggtgtcggc gatcgcggct
     9061 tcgtcttcga caatcagaat atgcggcatg ggcgtcctgc tcgtggcaac tcggcgagga
     9121 gcctgcggca ggctgcctgc agcgtcaagg gtgagcagac ggtgccgcgc tctgtaggag
     9181 cgggccatgc ccgcgagcga ctctcagata gacgagtaca tcgcggacaa gtccgctcct
     9241 acggccctca aacgagtttg aatcgcgctg tgttctgctc caacgcctga ctgccctggc
     9301 gcagctgttc ggcagcatta ctcaccgcct gcgcgccttc aagcagggcg ctggccgcat
     9361 gatcgacctg ctgaatattg ccgctgactt catcggcagt agccgcctgt tgctcggcgg
     9421 cagtggcgat ctgcgccagg cggtcagtca ccagttgcac cgactcgaca atggcattca
     9481 ggtcctcgcc catggccagc acgctgccaa catcgccttc ggcctggcgc acggcgtctt
     9541 ccatctgggt gaccgactgg ccgaccacgc ggtgcaggtt ggccacggtt tcggcaattt
     9601 ccgccgtgga gttctgcgta cgctgcgaca gcgaacgcac ctcgtcggcc accacggcaa
     9661 aaccgcggcc ctgctcgccg gcgcgcgcgg cttcgatggc ggcgttaagc gcgagcaggt
     9721 tggtttgttc ggcaatgccc ttgatcacat ccactacacg ggtgatctgc tcggtctgcg
     9781 tacgcagttg ctgcaaggtg gtggcactgt cgcccagacg gctgctgagt tgacgcatgc
     9841 tggcgctggt gcgggcgcta cgctggttgc tctgttcggc aatctcccgg gtctgcccgg
     9901 cttccacggc agcctgctca caactctgcg ccacgctctg cgcggtggcg gccatttcag
     9961 tggcggcagc ggcaatctgg ctcatctgcg cctgctgctg ctcgaccgca gccagggcgg
    10021 tttgcgcttc atggctgaga gactgcaccg tgccgccaag gcgctgactc tccaggtcga
    10081 cgccttgcaa ggagctgcgc aactgaccga ccgagatgtt caaggcacgg gcgatagccg
    10141 ccaggtcatc ttccccatgc acctccatgc gcacccgcag gtcgccgtcg cgcaagccct
    10201 cggcagcgct aatgatgctc gcagtgctgc ggcgaatcga ggcgttcagg cacagcagca
    10261 catagagcgc gatcagggtc agcacgccga atgcgaggat gatttctgtc atcttccatt
    10321 gcgcctcgct gcggtagtgc ccgagggttt cggcaaactg gcggtattgg gcgtcctgca
    10381 gtgcgcgcaa gtgtccttcc agcacggcga cccgctcgat aaattgcgcc ggggtcagcg
    10441 tcatcgggct ggcctgaaac atgtcgcgat caatatcact gaggaactgg tccaggccct
    10501 gctcggccgc agcaaagggc gcgtcgaggt tacgcatgac ccgcggcgcc tcttgcgcca
    10561 gggtggtgcg cgccttgatc aattggctgc gcgactcatc caacgcccgt cgcaggtcac
    10621 gcatcagcac gcggctttgc aaggtgaagt gctgggagaa caccgcgccg taaccctggc
    10681 tgacaaatcc acccaggttg tcgagcaaac gcggcagggt gaaggtcagc tgctccatca
    10741 tcaggtagct gtccagataa gggtcgagaa tcaacccgct atcggtcgcc acctgctcac
    10801 gcagagtcat aacctgtaag agggccttct ggtaacgctc aagcgcttca ggtaacggca
    10861 ccttggccat tgcgccaacc gccaattcgg cgcgcagcgt ttgcaggctc tggagatggg
    10921 cttgggtctg cggactgagc atatcgtctt gcaactgctc gatggcagca gtcagagcat
    10981 catcgagtgc ggcttcacgc tccttcaata atgcctcggc cgccgtgtcc gtgcccttcc
    11041 agcgtgcaga taacgtgcgc tgctctagaa cagcgcgctg caccgcacca attgattgca
    11101 cgcctcgcat gccctccagc tcgttatcga tcagcgccag gcgcccgagg tgatcgctac
    11161 tgatcaccca cagcgcatag gccaacggca gaacaaataa gacaaacacc aactgaaact
    11221 tgcgtgcgaa gctaaaacgc tccagcagcc gcacgcccgg tttgagtact ccagtcatac
    11281 acacctcaac ctgtgaagcg cgccaacttc aggggcgcgc caaagcaaaa cttcaagcaa
    11341 gaagcacgcc caagcagcgc gcctgcaggc ggtaatgtgt aatgagcata gatgagccgc
    11401 cgagccacga cctgccagcc agtcggcgag ctgcgtaagt gccaagaaac tctagcggga
    11461 aataccgctc cagagttcct caacattgcg gaaaccccac tcttcagcag actcacgacc
    11521 gacgatcttg tcgcgcccaa ccagcttgct caggtcgagg atttcctgcg ggatcggggt
    11581 tttcgatttg gccgagaaga acaccttgac cctcggcgcg agcaaggact tggcctgctg
    11641 ctccatcacc acgccgatac gcccgctctc cagacgcacc agcgaaccca ccgggtaaat
    11701 gcccacagtt ttgacaaagg cctgaaacac cacagggtcg aaatgcccct tccactccgc
    11761 catcttgcgg atcgactcgg ccgggtccca cccctgtttg tacggtcggt tggaggtgat
    11821 cgcgtcatac acgtcgcaca ccgcccccat acgcgcatac aggctgatct gctcgccttc
    11881 caggcgatgc gggtagcccg taccgtccat cttttcatgg tggtgcagac atacatccag
    11941 caccagcgcg ctgacctgct tactgtccag cagcatgcgc gaacctactt cgggatggtc
    12001 gcgcacggta gcgaattcgt tgtccgtcag cttgccaggc ttgttcagca cctcactggg
    12061 aatcgccatt ttaccgatat catgcagcag cccggccagc ccagcctcgc gcacctgccc
    12121 ctcgttcagg cccagattgc gcgccagggc aatcatcagt gcgcagaccg ccaccgaatg
    12181 catataggtg tattcatcgg cgtttttcag ccgcgccagg ctgattaacg cattggggtg
    12241 acgcatgatc gagtcggaaa tctcctcgac cagctccccg gcctgctcga actgcaacgc
    12301 ctggcccatg cgagcgtcac caaacatgct gaccaccgcg gctttggaac gctcgcagag
    12361 cttggccgca cgccgtacct catcgtccat cgacaccgcc aggttgactg gcgggctctg
    12421 tacagcagcc aaaagggcgg cctcggcttc ggcagcgact tcctcggggc tgacgctggc
    12481 cgtgccgacc gcgacatcca ggcccttgct gacatcgatc cacagctcac cgatgctgct
    12541 ggccttgatc cgcagcaggt cgttttccga actgagaaga aacttggatt tccagaaggg
    12601 gtggtccatc catgcgccac agaactcgtg gatatacata ccgacgcgga cgtcagccac
    12661 tgcgatgcac ttcaacatgg atggcgctcg aaggagaaag taacccggtg attgtcagac
    12721 aagcgtagat gaacaatcgc cagcactatg ctgacctgca gcaactaatg gccataggac
    12781 atcagtcgca ggccggcggc ttggaagcct tgccacgact gcttatcatt gccgctcaag
    12841 cgcaccacaa ggagttgcca tgcgccgtct gttgaccggt cttagcgtca ttattctgct
    12901 gttgctcaat acgctgatac tgatcggccc gctgctgttg atcgccctcg gcaagttcat
    12961 cctgcccggc aaagcactga aaaccgccag ttcacgcggg gtaatgtggg tggcggaaac
    13021 ctgggcagag ctcaacaagc tgatctttgc cacgctgttg ccggtgcatt gggacatacg
    13081 cggcaatgcc gacctgcgcc agagcacctc ctacttggtg atcagcaacc accagtcctg
    13141 ggtcgacatt ccggcactgg tgcaagcctt caaccgcaag acgccctact tcaagttctt
    13201 cctcaagcag cagctgatct gggtaccctt tcttggcgtg gcgttctggg cgctggatta
    13261 cccctttatg aagcgttact ccaaggcatt tctggccaag catcccgaac tcaagggtca
    13321 ggacctggaa atcaccaaac tcgcctgtga gaagttcaag gacctgccgg tgaccgtggt
    13381 caattacctc gaaggcaccc gctgtacccc ggcaaagcag gcgcagcaag gctcgcccta
    13441 tcagtatctg ctcaagccga aagccggcgg tgtggccttt gttctggcgg cgctgggcga
    13501 gcagctggac accgtgctgg acgtgaccgt ggtgtatcca ggcgcacgga ttcccggctt
    13561 ttgggatttg atcagcggcc aggtggacaa ggttatcgtc gatatccaga cccgcgacct
    13621 cgacccggcg ctgtggcagg gcgattacga gaacgacccg gtattccgcc agtttgtgca
    13681 ggattgggtg accaagatgt ggcaagacaa agatgcgcgg attgccgcga ttcatgcgga
    13741 gattgaggtg tagggtggac atggcacagc cttgtccacc acaggcatca tggcggacaa
    13801 gccttcggca tgtccgccct acggatgatt gatgacttgg tttatgccgc gctaaacatc
    13861 ttgtgcgggt cgatgacgaa tttcttcggc acgccggcat cgaactcgcc gtaaccgcgc
    13921 ggcgcgtcgt ccaggctgat gacctgcacg ccgaccactt cggcgatgtt gatacggtcc
    13981 cacatgatcg cctgcatcag tgcgcggttg tacttcatca ctggggtctg gccggtgtgg
    14041 aagctgtggg atttggccca gccgaggccg aagcggatgc ttaaggaacc gatcttcgcc
    14101 gcggcatcca ccgcacccgg atcttcggtg acgtagaggc cgggaatacc gatcttaccg
    14161 gccacgcgca ccacgcccat cagcgagttg agtaccgtgg ccggcgcttc gtgttgcacg
    14221 cctgcatggc cgtgaccacg ggcctcgaag cccaccgcat ccactgcgca atccacttcc
    14281 ggctcaccca gcagggcggc gatctgttcg tgcagcgggg tgtccttgga caggtcggca
    14341 atctcgaaac ccaccgcctt ggcatgcgcc agacgcaccg gattaacgtc accaacaatc
    14401 accaccgccg cgccgagcag acgcgccgaa gcggctgcgg ccagaccgac cgggccggca
    14461 ccagcgatat acacggtgct gcctgggcca acaccggcgg tcactgcgcc gtgataacca
    14521 gtgggcagta tgtcggacag gcaggtcagg tcgcggatct tctccatggc cttgtcgcga
    14581 tccggcagtt tcagcaggtt gaaatcggcg tacggcacca tggcgtactc agcctggcca
    14641 ccggtccagt cacccatatc gacgtagccg taggcaccgc cggcacgcgc cgggttgact
    14701 gacaggcaca cgccagtgtg ctgttccttg cacgaacggc aacggccgca agccacg
//
LOCUS       DASRUM010000144         3263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2405495, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000144 DASRUM010000000
VERSION     DASRUM010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2405495"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..78
                     /locus_tag="VFF22_09630"
     CDS             <1..78
                     /locus_tag="VFF22_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZX17107.1"
                     /translation="LLESRSLSGEEKKLLEEYVRQRDDS"
     gene            119..469
                     /gene="rplS"
                     /locus_tag="VFF22_09635"
     CDS             119..469
                     /gene="rplS"
                     /locus_tag="VFF22_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZX17108.1"
                     /translation="MTNKIIQQLEAEQMGKEIPTFAPGDTIVVQVKVKEGDRQRLQAF
                     EGVVIAKRNRGLNSAFTVRKISNGVGVERTFQTYSPLVDSLAVKRRGDVRKAKLYYLR
                     DLSGKAARIKEKLG"
     gene            complement(544..1215)
                     /locus_tag="VFF22_09640"
     CDS             complement(544..1215)
                     /locus_tag="VFF22_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17109.1"
                     /translation="MSSQQHSRLGQILINKGLINRSQLDAAIQLQLTNHKRLGETLIE
                     QGWLTERQLKKALSKQNNLRLAATLVAALLSPFQIASADAPRNLPAISQEHTPRGLHP
                     LTDMEMSNVSAQGLDDVLQGLFLQAGSGDGMGTISQLSKLVMPVLSSLEAETSMKDVQ
                     YDTSKLTSVINADGSVNVRLPSSIGELRFDNIRVAGAPQGQSMGSLSLHDIDLSQASL
                     KISLR"
     gene            1406..2302
                     /gene="xerD"
                     /locus_tag="VFF22_09645"
     CDS             1406..2302
                     /gene="xerD"
                     /locus_tag="VFF22_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HZX17110.1"
                     /translation="MAAIDHPLIDRFLESLWLEKGLSAHTQAAYRSDLALFNGWLQVR
                     DVQLPDAGRELILDHLAWRLNEGYKARSTARLLSGLRGFYRFLLREQLISRDPTLQVD
                     LPQLGRPLPKSLSEADVEALLAAPELDDPIGLRDRAMLEVLYACGLRVSELIGLTLEQ
                     VNLRQGVLRVFGKGSKERLVPLGEEAIAWIERYSKEARPFLLDGKPSDVLFPSLRGEQ
                     MTRQTFWYRIKHQAKVAGIAKSLSPHTLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVARARLQELHAQHHPRG"
     gene            2396..3121
                     /gene="dsbC"
                     /locus_tag="VFF22_09650"
     CDS             2396..3121
                     /gene="dsbC"
                     /locus_tag="VFF22_09650"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003282959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HZX17111.1"
                     /translation="MRFNRIVVALALGLVSGVGVAADADQAIRNSLKAIDQALPIEAI
                     AESPMPGVYQVELAGGRQLYTSADGQFLLQGYLFQVKDGKPVNLTEAAESRGVVKLLD
                     AIPAAEMVVFAPKQPKTHITVFTDTDCGYCQKLHSEVPELNRLGIEVRYLAFPRQGLE
                     SAAAKELSNVWCAKDQQDAMNRAKSRQSVADAQCDSPVAKQYALGQMIGVSGTPAIVL
                     ANGKIIPGYQPAPQLAKIALESK"
BASE COUNT          612 a          854 c         1026 g          771 t
ORIGIN      
        1 cttctggaaa gccgctcgct ttctggagaa gagaaaaagc tgctggagga atacgtccgc
       61 cagcgggacg atagttaatg tatcgatgac cggccgaggc ctgtcttagg agcgcagcat
      121 gaccaacaag attattcagc agcttgaagc tgagcaaatg ggcaaagaga tcccaacttt
      181 cgcgccaggt gacaccattg ttgtccaggt taaagtgaaa gaaggcgacc gtcagcgtct
      241 gcaggcgttt gaaggcgttg taattgccaa gcgtaaccgt ggtctgaaca gtgctttcac
      301 tgttcgtaag atctccaatg gtgttggcgt tgagcgtacc ttccagacct acagcccgct
      361 ggttgacagc ctggccgtta agcgtcgcgg cgacgtgcgt aaagccaagc tgtactacct
      421 gcgtgacctg tccggtaaag cagcgcgcat caaggaaaaa ctgggctaag tctcagtctt
      481 ccaataaaaa agcagccttc gggctgcttt tttcgtttca gcggtctgaa taccgcgttg
      541 cggctagcgc aggctgatct tcagtgaggc ttgtgacagg tcgatgtcat gcaggctcag
      601 gctgcccatg ctctggccct ggggagcgcc agcaacgcgg atattgtcga accgcagctc
      661 gccaatcgag ctgggcaggc gcacattcac cgagccgtct gcgttgatca ctgaggtcag
      721 tttgctggtg tcgtactgca cgtctttcat cgaggtttcc gcctcaaggc tgctcagtac
      781 cggcatcacc agtttgctca gttggctgat ggtgcccatg ccgtcaccgc tgccggcttg
      841 gagaaacagg ccttgcaata cgtcgtccag gccctgggcg ctgacattgc tcatttccat
      901 gtccgtcaga gggtgcaggc cgcgcggcgt gtgttcctgg ctgatagcgg gcaggttgcg
      961 tggtgcatcg gcgctggcga tttggaaggg gctaagcagt gcggcgacca gtgtggcggc
     1021 caggcgaagg ttgttctgtt tgctgagtgc ctttttcagc tggcgctcgg ttagccagcc
     1081 ttgctcaatc agcgtttcgc cgagacgttt gtggttggtc agttgtagtt gaatagccgc
     1141 atccagctgg ctgcggttaa tcaatccttt gttgatcaga atctgaccaa ggcgtgagtg
     1201 ctgttggctg gacatgggtg ggtaccgttg tgcagtagtg gcgtgatgcg atcatggtct
     1261 gcaaatcacg gtctgtcacc ctcccaaaga tagggtgacc ggagctttct tcgctgtttt
     1321 agccggccgt tggtcggtgc gcttagtgct ggcccgtggc gcagcgctcg tagacactag
     1381 cggatatatc gtttgaggtt tcttgatggc agctatcgat catccgctga ttgaccgttt
     1441 tcttgagtcg ttatggttgg aaaaaggcct gtcggcccac actcaggccg cctaccgcag
     1501 cgacctcgcg ctgttcaatg gctggctgca ggttcgcgat gtgcaattac cggatgcagg
     1561 gcgtgagctg attctcgatc acttggcctg gcgtctgaat gagggttaca aggcgcgttc
     1621 taccgcgcgc ctgctgtcgg gcttgcgcgg tttctaccgg tttctcttgc gtgagcagct
     1681 gatcagccgt gatccaaccc tgcaggtgga tttgccgcag ttgggccggc cgctgccgaa
     1741 atcgctgtcc gaagccgatg ttgaggcctt gctggccgcc cccgagctgg atgacccgat
     1801 tggtctgcgt gatcgggcga tgcttgaggt gctgtatgcc tgtggtctgc gcgtcagtga
     1861 gttgattggc ttgacactgg agcaggtcaa tctgcgtcag ggtgtgttgc gcgtcttcgg
     1921 caagggcagc aaggagcgcc tggtgccgct gggtgaagag gcgattgcct ggattgagcg
     1981 ttacagcaag gaggcgcgac cgtttctgct cgatggcaaa cccagtgacg tgctgttccc
     2041 cagtttgcgt ggtgagcaga tgacccggca gaccttctgg tatcgcatca agcaccaggc
     2101 caaggtggcg gggattgcca agagtctgtc gccgcacact ctgcgccatg catttgccac
     2161 gcacctgctt aatcacggtg ctgatttgcg cgtggtacag atgctgttgg ggcacagcga
     2221 tctatcgacc acgcagatct acacccatgt tgcccgcgcg cggctgcagg agttgcatgc
     2281 gcagcaccac ccgagaggct gagagcgccg tctggcctgc gatgatcggc ctatgtgggc
     2341 ttctatggta ggctgcgccc atcctttgtt agatcgtctt tcgccaggag tttgtatgcg
     2401 ttttaaccgc attgttgtcg cgttggcctt gggtctggtc agcggcgtgg gtgttgccgc
     2461 cgatgctgat caggctattc gcaatagcct caaagctatc gatcaggctc tgccgattga
     2521 ggcgattgcc gaaagcccga tgcctggcgt ttatcaggtt gagttggccg gtggtcgaca
     2581 gctgtacacc agtgctgatg gtcagttcct gttgcagggt tacctgttcc aagtcaagga
     2641 cggcaagccg gtgaatctca ctgaggcggc ggaaagccgt ggtgtggtca agttgttgga
     2701 tgccatcccg gctgcggaaa tggtggtgtt tgcgcccaag caaccgaaaa cccatatcac
     2761 tgtatttacc gacaccgatt gcggctattg ccagaagctg cacagcgaag tgcctgagct
     2821 caatcgtctc ggcattgaag tgcgttacct ggccttcccg cgtcagggct tggaaagcgc
     2881 ggcagccaaa gaattgtcga atgtctggtg cgccaaggac cagcaggacg ctatgaaccg
     2941 cgcgaaaagc cgccaaagcg tggcagatgc gcagtgcgac agcccggttg ccaagcagta
     3001 cgcgttgggc cagatgatcg gggtcagcgg tacgccggcc atcgttctgg ccaacggcaa
     3061 gattattccc ggctatcaac cagcaccgca gctggcgaaa attgccttgg aatccaaatg
     3121 atctgaggcg tgcgcctgat tgactaataa acagccgctt cgtatggtgc ggctcttttc
     3181 tacggccggg gcttgctccg gccgttttac gcagtgcaga tggggagttc agtgtgaaac
     3241 cggtgaaagt aggcatctgt ggg
//
LOCUS       DASRUM010000145         6282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2239887, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000145 DASRUM010000000
VERSION     DASRUM010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2239887"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VFF22_09655"
     CDS             complement(<1..720)
                     /locus_tag="VFF22_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HZX17112.1"
                     /translation="MKRIHVAAAVIRGVDGQILLAKRPEDKHQGGLWEFPGGKVEASE
                     TVQAALARELEEELGIRPSAARPLIQVRHDYPDKQVLLDVWEVSAFTGEPHGAEGQPL
                     AWVSPRQLGDYEFPAANRPIVAAARLPERYLITPEGLSGPELLRGIQAALKSGIRLLQ
                     LRAPAMFDAQYRDIAVDALGLCAGKAQLMLKGPLEWLGDFPAAGWHLTSQQLRELSSG
                     GRPFPAQRWLAASCHTAQELEL"
     gene            complement(717..1349)
                     /locus_tag="VFF22_09660"
     CDS             complement(717..1349)
                     /locus_tag="VFF22_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZX17113.1"
                     /translation="MSLTLVIGDKTYSSWSLRAALALELTGAPYAEQLITLNRPDTRA
                     QILQHSPTGKVPLLISDDGAIWDSLAIGEYLAECFPAAQLWPQALAARALARSACAEM
                     HSGFVALRSHLPMDLKRNQALSEIPADAVADIQRICVLWAECRQRFGQDGGFLFGPAS
                     LADAFFAPVAARLRSYQVALPAEAAAYVETIYQWPAFQRWYQAAQEEHLA"
     gene            complement(1476..2693)
                     /gene="argJ"
                     /locus_tag="VFF22_09665"
     CDS             complement(1476..2693)
                     /gene="argJ"
                     /locus_tag="VFF22_09665"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HZX17114.1"
                     /translation="MAVGLGPLSTLHPVPGFELGIASAGIKRPGRKDVVVMRCAEGSN
                     VAGVFTLNAFCAAPVILAKKRVLGSVRYLLTNTGNANAGTGEPGLQNAMRTCAALAQL
                     TAVDEGAVLPFSTGVIGEPLPVEKIEGALQAALDDLSENHWAEAATGIMTTDTLPKGA
                     SRQFVHDGVTVTVTGISKGAGMIRPNMATMLGYIATDAKVARSVLQDLVRDAANKSFN
                     RITIDGDTSTNDCCMLIATGQAALPEITQASGDLFAKLKEAVFSVCMEVAQAIVRDGE
                     GATKFVTVQVNGGATHQECLDVGYAVAHSPLIKTALFASDPNWGRILAAVGYAGVAQL
                     DVSLIDVFLGDVCIASRGCRAASYTEEQGAAVMAKEEITIRIELGRGGCSETIWTTDL
                     SHEYVKINAEYRT"
     gene            complement(2835..5567)
                     /gene="secA"
                     /locus_tag="VFF22_09670"
     CDS             complement(2835..5567)
                     /gene="secA"
                     /locus_tag="VFF22_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZX17115.1"
                     /translation="MFAPLLKKLFGSKNEREVKRMLKAVQAVNAFEEQMLALSDEQLR
                     AKTEEFKARLAKGETLDQLLAEAFAVAREAGKLVMGMRHFDVQLIGGMTLHEGKIAEM
                     RTGEGKTLVATLAVYLNALSGKGVHVVTVNDYLARRDANWMRPLYEFLGMSVGIVTPF
                     MPPEEKREAYAADITYGTNNEFGFDYLRDNMAFSLEDKFQRELNFAVIDEVDSILIDE
                     ARTPLIISGQAEDSSRLYTEINKLIPRLKLHVEEEEGVVTQEGHYKVDEKARQVELNE
                     AGHQYVEEMLTEVGLLAEGESLYSAHNLGLLTHVYSGLRAHKLFNRNVEYIVQNNQVL
                     LIDEHTGRTMPGRRLSEGLHQAIEAKEGLPIQAESQTLASTTFQNYFRLYNKLSGMTG
                     TADTEAFEFHQIYGLAVIVIPTNRALARKDFNDLVYLTAEEKYAAIVTDIKESMALGR
                     PVLVGTATIETSEHMSRLLNDEGIEHKVLNAKFHEKEAEIIAQAGRPGALTIATNMAG
                     RGTDILLGGNWEVEVANLENATPEQIAQIKADWQKRHQQVIESGGLHVIASERHESRR
                     IDNQLRGRSGRQGDPGSSRFYLSLEDSLMRIFASDRVKNFMKALGMQSGEAIEHRMVS
                     NAIEKAQRKVEGRNFDMRKQLLEFDDVANEQRKVIYHMRNTLLAADNIGETIAEFRQE
                     VLDNTINAHIAPQSLPEQWDIAGLEEALYSSFNLRLPIQQWLDDDHKLYEETLRARIL
                     EELVNAYNEKENEASAEALRTFEKQILLRVLDDLWKDHLSTMDHLRHGIHLRGYAQKN
                     PKQEYKRESFTLFQELLDSIKRDTIRVLSHVQVRREDPAEEEARLRREAEALAERMQF
                     QHAEASALLPQATEEEGDQAVAVAPVRTEPKIGRNEPCPCGSGKKYKHCHGQVN"
     gene            complement(5685..>6282)
                     /locus_tag="VFF22_09675"
     CDS             complement(5685..>6282)
                     /locus_tag="VFF22_09675"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZX17116.1"
                     /translation="DASEFDFSLNVGQGGAEDALGDAAYAPPPFMDELDRLSLRLDSR
                     EQLLEVLEQLIGERQLNEAEYLSGMPVRHGYMSSPFGRRVHPLTGRNTQHKGVDFAAK
                     PGSDVVSVAAGVVSFSGVKSGYGHTVEISHADGYTTVYAHNQSNLVQIGDLVQRGQTI
                     AKVGRSGRASGYHVHFEVTKDGRVVNPASYIARVNDVE"
BASE COUNT         1232 a         1931 c         1861 g         1258 t
ORIGIN      
        1 caattccagt tcttgcgccg tgtggcagga ggccgcgagc cagcgctgcg ctggaaacgg
       61 tcgaccgcca gagctcaatt cacgcaactg ctgagacgtc aggtgccagc cagcggcggg
      121 aaagtcgccc agccactcca gcggcccctt gagcatcagc tgcgccttgc cggcacaaag
      181 gccgagggca tccacggcaa tgtcacggta ctgcgcatca aacatcgccg gagcacgcag
      241 ttgcagcagg cgaatgccgc ttttcagcgc ggcctggata ccgcgcagca gctcaggtcc
      301 gctcaggccc tcgggggtga tcagataacg ctcgggcaaa cgcgccgccg cgacaatcgg
      361 ccgattggct gcaggaaact cataatcgcc caactgccga gggctaaccc aggccagcgg
      421 ctggccttcg gcgccgtggg gctcaccggt aaacgccgag acttcccaga cgtccagcag
      481 cacctgcttg tccgggtaat catgacgcac ctgaatcaac ggccgcgcgg cgctggggcg
      541 aatacccagt tcctcttcca gttcacgggc cagggctgcc tgcaccgttt cgctggcctc
      601 gaccttgcca ccgggaaact cccacagccc gccctgatgc ttatcctcag gacgtttggc
      661 gagcagtatc tgcccatcca caccgcgaat aaccgcagcg gctacatgaa tacgcttcac
      721 gccagatgct cctcctgggc ggcttggtac cagcgctgaa acgccggcca ctgatagatg
      781 gtctcgacat aggcggccgc ctctgccggc aaagccacct gataactgcg caggcgcgct
      841 gccaccgggg caaagaaggc atcggccagg ctagccggac caaacagaaa gccgccgtcc
      901 tggccaaagc gctgccggca ctcggcccac aggacgcaga tgcgctggat atcggccacg
      961 gcatcggccg gtatctcaga cagcgcctga ttgcgtttga ggtccatcgg cagatggctg
     1021 cgcagtgcga caaagccgct gtgcatctcg gcgcaggcac tgcgcgccag cgcacgggca
     1081 gcaagggctt gcggccacag ctgagcagcg ggaaagcatt cggccaggta ttcgccaatc
     1141 gccagagaat cccagatggc gccatcgtcg ctaatcagca acggcacctt accagtcggc
     1201 gagtgctgca aaatctgcgc gcgggtatcg gggcggttca gggtaatcag ctgctcagca
     1261 tagggcgcgc cggtcagctc cagggccagc gccgcacgca gcgaccaaga cgaataggtt
     1321 ttgtcgccga taaccagagt aagtgacatg ggcttcgctc gatacaagaa ggtcgaatga
     1381 ccttaaaagg cgactcccgc aatagcaaat tcccgttgct tatgggggca taaacaacgg
     1441 gaatgcgggt cgtcaaactg caggtaagtg accgatcagg tgcggtattc ggcgttgatc
     1501 ttcacgtact cgtgggacag gtcggtggtc cagatggtct cgctgcagcc accacggccc
     1561 agttcgatgc ggatggtgat ctcttccttg gccatcactg cggcgccctg ctcttcggtg
     1621 taactggccg cgcggcagcc acggctggcg atgcacacgt caccgaggaa tacgtcgatc
     1681 aggctgacat ccagctgcgc cacaccagcg taaccgacgg cggcgagaat gcgcccccag
     1741 ttcgggtcgg aggcaaacag cgcggtcttg atcagcggcg aatgggccac cgcgtaacca
     1801 acatccaggc actcctggtg agtcgcgccg ccgttgacct gcacggtgac gaacttggtt
     1861 gcgccttcac cgtcgcggac gatggcctga gccacttcca tgcacacgct gaataccgct
     1921 tccttgagct tggcaaatag atcaccactg gcctgggtga tttccggcag ggccgcctgg
     1981 ccagtggcga tcagcatgca gcagtcgttg gtcgaggtat caccgtcgat ggtgatgcga
     2041 ttgaacgact tgttcgccgc atcacggacc aggtcctgca gcacgctgcg cgcgaccttg
     2101 gcgtcagtgg cgatataacc cagcatggtc gccatgttcg gacgaatcat cccggcgcct
     2161 ttgctgatgc cggtcacagt gacagtgaca ccatcgtgaa caaactggcg gctggcaccc
     2221 ttgggcaggg tgtcggtagt catgatgccg gttgcagctt cggcccaatg gttctcggac
     2281 aagtcgtcga gagcagcctg cagcgcgcct tcgatcttct cgaccggcag cggctcgcca
     2341 atcacccccg tggagaacgg cagcacagcg ccttcatcca ctgcggtcaa ctgagccaga
     2401 gcagcgcagg tgcgcatggc attctgcagg cccggctcgc cggtgccggc gttggcgtta
     2461 ccggtgttgg tcagcaggta acgcacactg ccgagcacac gcttcttggc caggataacc
     2521 ggcgcggcac agaacgcatt gagggtgaac acaccggcga cgttggagcc ttcggcacat
     2581 cgcatcacca ccacatcctt gcgtcctggg cgtttgatgc ccgcagaagc gatgcccagc
     2641 tcgaaacctg gaaccgggtg cagggtagac aacgggccaa gaccaacagc catagagaac
     2701 gctccttgag gtggtgatac gtctgattgc gatggtaaaa cgccgcgagc ggtcagaccg
     2761 ttcgcggcgt taatcaagcg gggtcaagca tagccaactt cactgtcggc tcaacccgcg
     2821 ctacagcggt cggcttagtt aacctgaccg tggcagtgct tgtacttctt gccggaacca
     2881 caagggcacg gctcgttgcg gccgatcttc ggctcagtgc gcaccggcgc aacggcaacc
     2941 gcctgatcac cctcttcttc agtggcttgc ggcagcagtg ccgaggcttc ggcgtgctgg
     3001 aactgcatcc gctcggccag agcttcggcc tcgcggcgca ggcgggcctc ttcctcggcc
     3061 ggatcttcac gacgaacctg cacatgcgat agcacacgga tggtgtcgcg cttgatcgaa
     3121 tccagcagct cctggaacag ggtgaaggac tcgcgcttgt attcctgctt ggggttcttc
     3181 tgcgcatagc cacgcaggtg aataccgtga cgcaggtgat ccatggtcga caggtgatct
     3241 ttccacaagt cgtcgagcac acgcagcaga atctgcttct cgaaggtgcg cagggcttcg
     3301 gcgctggctt cgttttcttt ttcgttgtaa gcgttgacca gctcttcgag aatgcgcgca
     3361 cgcagggtct cttcgtacag tttgtggtca tcgtcgagcc actgctggat cggcaggcgc
     3421 aggttgaagc tgctgtacag cgcctcctcc agaccggcga tatcccactg ctcaggcagc
     3481 gactgcggcg cgatatgtgc gttgatggtg ttatccagca cttcctgacg gaactcggcg
     3541 atggtctcgc cgatgttgtc ggcagccagc agcgtgttgc gcatgtgata gatcactttg
     3601 cgctgctcgt tggccacgtc gtcgaattcc agcagttgct tacgcatgtc gaagttgcgc
     3661 ccttcaacct tacgctgcgc cttctcgatg gcgttgctga ccatgcggtg ttcaatggct
     3721 tcaccggact gcatgcccag ggccttcata aagttcttca cccgatccga ggcgaagata
     3781 cgcatcagac tgtcttccag cgacaggtag aaacggctgg aacccgggtc cccctgacgg
     3841 ccggaacgac cgcgcagctg gttgtcgata cggcgcgatt catggcgctc ggaggcgatc
     3901 acatgcagac cgcccgactc gatcacctgc tggtgacgct tctgccaatc ggccttgatc
     3961 tgcgcgatct gctcaggcgt ggcattttcc aggttggcaa cttcgacttc ccagttgccg
     4021 cccagcagga tgtcagtacc gcgaccggcc atgttggtgg caatggtcag cgcgcccgga
     4081 cgaccggcct gggcaatgat ttccgcttcc ttctcgtgga acttggcgtt gagtaccttg
     4141 tgctcgatgc cttcatcatt tagcagcctg gacatgtgct cggaagtttc gatggtggcc
     4201 gtaccgacca gcaccggacg gcccagcgcc atgctttcct tgatgtcggt gacgatggcg
     4261 gcgtacttct cttccgcagt caggtagacc agatcgttga agtccttacg tgccagggca
     4321 cggttggtcg ggatcacgat cactgccaag ccgtaaatct gatggaactc gaatgcttcg
     4381 gtatccgccg taccggtcat gccggacagc ttgttgtaca ggcggaagta gttctggaag
     4441 gtggtcgagg ccagggtctg gctctcggcc tggatcggca ggccttcttt ggcctcgatc
     4501 gcctggtgca ggccttcgga caaacgacga ccgggcatgg tgcggccggt gtgttcgtcg
     4561 atcagcagca cctggttgtt ctgcacgatg tattcaacgt tgcgattgaa cagcttgtgc
     4621 gcacgcaggc ccgagtaaac gtgggtcagc aggcccaggt tatgcgccga atacaggctc
     4681 tcgccctcgg ccagcaggcc gacttcggtg agcatttctt caacgtactg gtggccagct
     4741 tcgttcagct caacctggcg ggccttctcg tcgaccttgt agtggccttc ctgcgtgacc
     4801 acgccctctt cttcttcaac atgcagtttg aggcgaggaa tcagcttgtt gatctcggtg
     4861 tagagccgcg agctgtcttc ggcttggccg gagatgatca gtggagtacg cgcttcgtcg
     4921 atcagaatgg agtcaacttc gtcgattacg gcgaaattca gctcacgctg gaatttgtct
     4981 tccaggctga aagccatgtt gtcgcgcagg taatcgaaac cgaattcgtt gttggtgccg
     5041 taggtgatat cggcggcgta ggcttcacgc ttctcttccg gcggcataaa gggggtaacg
     5101 atacccacgc tcataccgag gaactcatac agcggacgca tccagttggc gtcgcgacga
     5161 gccaagtaat cgttgaccgt caccacatgc acgcccttgc cggacagcgc attgaggtac
     5221 accgccaggg tcgccaccag ggtcttaccc tcaccggtgc gcatctcggc gatcttgcct
     5281 tcgtgcaggg tcataccacc aatcaactgc acgtcgaagt ggcgcatgcc catcaccagc
     5341 ttgccggctt cgcgggctac cgcaaaggct tcggctagca gctggtcgag ggtttcgcct
     5401 ttggccagac gggctttgaa ctcctccgtc ttggcacgca gctgctcatc agaaagggca
     5461 agcatctgct cctcgaaggc attgacggct tgtaccgcct tgagcatgcg tttgacttca
     5521 cgctcgttct tgcttccaaa gagttttttc aacaaaggcg caaacatatc gacaggatct
     5581 tccaggcgta tggattgagg gcggctgaac agcgacccat gcagccttta tggctgcgtg
     5641 cgaagggggc attctacccg gaaacgcaaa cgaggaaagc ggcgttattc gacgtcgtta
     5701 acccgggcta tatagctggc tggattgacc actcggccat ccttggtcac ttcgaagtgc
     5761 acatggtaac cactggcgcg accgctgcgg ccaaccttag caatcgtctg gccgcgctgc
     5821 accaagtcac caatctgtac caagttgctc tgattgtgcg catacaccgt ggtgtagcca
     5881 tcggcatgac tgatctccac ggtatgccca taacccgact tgaccccgga gaagctcaca
     5941 acacccgccg caacagacac cacatcgctg cccggcttgg cggcaaaatc aacgccttta
     6001 tgctgagtat tgcgcccagt cagtgggtgc acgcggcgac caaatggcga ggacatatag
     6061 ccatgacgta ccggcatgcc agacaggtat tcggcttcat tcagctgtcg ctcgccgatc
     6121 agttgctcca gcacttccag caactgttcg cgactgtcca ggcgcaggct caggcgatca
     6181 agctcatcca taaacggcgg cggcgcatac gcggcatcgc ccagggcatc ttccgcaccg
     6241 ccctggccaa cattgagaga gaaatcaaat tcgctggcat cc
//
LOCUS       DASRUM010000146         4765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_404885, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000146 DASRUM010000000
VERSION     DASRUM010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404885"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1239
                     /gene="queA"
                     /locus_tag="VFF22_09680"
     CDS             190..1239
                     /gene="queA"
                     /locus_tag="VFF22_09680"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003175977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HZX17117.1"
                     /translation="MRVADFNFELPDALIARHPLAERRASRLLVLDGPTGELAHKQFS
                     DLLDYLRPGDLMVFNNTRVIPARLFGQKASGGKLEILVERVLDAHRVLAHVRSSKSPK
                     PGSRILIDGGDEAEMVARHDALFELRFSEEVLPLLERVGHMPLPPYIDRPDEQADRER
                     YQTVYADRAGAVAAPTAGLHFDQQLLAAIAEKGVERAFVTLHVGAGTFQPVRVERIED
                     HHMHSEWLEVSQDVVDAVAACRARGGRVIAVGTTSVRSLESAARDGMLKAFSGDTDIF
                     IYPGRPLHVVDALVTNFHLPESTLLMLVSAFAGYPETMAAYQAAIAGNYRFFSYGDAM
                     FITRNPAPRGPEEQL"
     gene            1254..2369
                     /gene="tgt"
                     /locus_tag="VFF22_09685"
     CDS             1254..2369
                     /gene="tgt"
                     /locus_tag="VFF22_09685"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HZX17118.1"
                     /translation="MSFELLATEGKARRGRLTFPRGVVETPAFMPVGTYGTVKGMLPR
                     DIEAIGAQIILGNTFHLWLRPGTEVIKKHGDLHDFMQWQGPILTDSGGFQVFSLGAMR
                     KIKEEGVYFASPVDGAKVFMGPEESMQVQRDLGSDIVMIFDECTPYPAEFDVAKRSME
                     LSLRWAKRSKEAHGESTAALFGIVQGGMHEELRKVSLDGLEEIGFDGYAIGGLSVGEP
                     KDEMIKVLDYLPAQLPADKPRYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLF
                     VDTGVLKIRNAFHKHDDSPLDSTCDCYTCKHFSRAYLHHLDKCGEMLGSMLNTIHNLR
                     HYQVLMAGLREAIQQGTLAAFVDAFYAKRGLATPSLD"
     gene            2409..2744
                     /gene="yajC"
                     /locus_tag="VFF22_09690"
     CDS             2409..2744
                     /gene="yajC"
                     /locus_tag="VFF22_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HZX17119.1"
                     /translation="MSFFIPAAFAEGAAPAAGPAGSGFEWVFLVGFLVIFYLMIWRPQ
                     AKRAKEHKGLISGLQKGDEVVTSGGIAGKVSKVSDDFVVIEVSDTVELKIQKVAIAAS
                     LPKGTLKAI"
     gene            2807..4675
                     /gene="secD"
                     /locus_tag="VFF22_09695"
     CDS             2807..4675
                     /gene="secD"
                     /locus_tag="VFF22_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZX17120.1"
                     /translation="MLNKFPLWKYLLILAVLAIGFLYSAPNLYPDDPAIQISGNSSAM
                     RVEQADLDRASRALEAAGISVKAVSLANQGRAGLLRLNSAEDQLPAKELVRKALSDEF
                     VVALNLAQTTPDWLRNLGGSPMKLGLDLSGGVHFLMEVDMDKALDARRKVYEGEVKSL
                     LRKERVRYRSLPELDGAIQLGFADEAALEKAQQLIRKDFTDFDLTTVQRGEQQVLRLA
                     LTQAKLVEIREYSIKQNLTTVRNRVNELGVAEPLVQRQGANRIVVELPGVQDTAEAKR
                     ILGKTANLEFRLAAEPDAAKAATESFEFREEGRPPAQLERDLIITGDQVTDAQASFDE
                     NGSPQVNIRLDGRGGDLMNRATRNNVGRSMAVIFIEQKPVTRYVRQVVDGVEKEVELQ
                     TFVEEKKIISLATIQSPLGSQFRITGLDGQGESSELALLLRAGGLAAPMYFAEERTIG
                     PSLGADNIAKGISASLWAMLFVSLFIIAIYRFFGVLATIALGLNMVLLLALMSLLSAT
                     LTLPGIAGIVLTMGMAVDANVLIFSRIREEIANGMSIQRAIHEGFDRAYSAIVDANLT
                     TLLVGGILFAMGTGPVKGFAVTLSLGILTSMFTAILVTRCMVNLTCGGRDFKKLWI"
BASE COUNT          885 a         1350 c         1505 g         1025 t
ORIGIN      
        1 gggggagact cgaactccca cttctttcga aaacggattt tgaatccgcc gcgtctaccg
       61 gttccgccac cggggcacaa tggcggcgca gtatagggat ggtgttgggg ttggtcaatc
      121 cacttgcgtg gtcagaatgc atgatttccg gtaagctttg ccgccctgct agacgacccc
      181 cttccgacca tgcgcgttgc cgacttcaat ttcgagctgc ctgacgctct gattgcccgc
      241 cacccccttg ccgaacgccg tgccagccgt ttgctggtgc tcgatgggcc gacaggtgag
      301 ctggctcata aacagttcag tgatctgctg gattacctgc gccctggcga tctgatggtg
      361 tttaacaaca cccgagtaat tccggcgcgg cttttcggcc agaaggcttc gggcggcaag
      421 ctggagattc tggtcgagcg cgtgctggac gctcatcgcg tgctggccca tgtgcgttcg
      481 agcaagtcgc cgaagccggg ctcgagaatc ctgattgacg gtggtgacga ggccgaaatg
      541 gttgcgcgtc acgatgcgtt gttcgagctg cgctttagcg aagaagtgct gccgctgctg
      601 gagcgggtcg ggcatatgcc gctgccgccc tatattgatc ggccggatga acaggccgac
      661 cgcgagcgct atcagacggt gtatgccgac cgtgccggcg ctgtggcggc gcccaccgcc
      721 gggctgcatt ttgatcagca gctgctcgcg gcaattgccg agaagggcgt tgagcgggcg
      781 ttcgtcacct tgcacgtcgg tgctggcacc tttcagccgg tgcgagtcga gcgcatcgaa
      841 gaccaccata tgcacagcga gtggctggag gtcagccagg atgtggtcga tgccgtggcc
      901 gcctgtcgcg cgcgtggcgg tcgggtgatt gccgttggca ccaccagcgt gcgttcgctg
      961 gaaagcgccg cgcgcgatgg catgctcaag gcgtttagtg gcgacaccga tatttttatc
     1021 tacccaggcc ggccgctgca tgtggtcgat gcgctggtga ccaacttcca tttgccggaa
     1081 tcgaccctgc tgatgctggt gtcggcgttt gccggttacc cggaaaccat ggcggcctat
     1141 caggcggcaa ttgccgggaa ttatcgtttt ttcagttacg gtgatgccat gttcatcacc
     1201 cgcaaccccg caccgcgcgg gccagaggaa cagctatgac gcgcacttgc cgcatgtctt
     1261 tcgaattgtt ggcgaccgag ggcaaggcgc gccgtggtcg gctgactttc ccccgtggcg
     1321 tggtggaaac cccggcgttt atgccggtgg gcacctacgg cacggtcaag ggcatgctgc
     1381 cgcgggatat cgaggcgatt ggtgcgcaga tcatcctcgg caacaccttc cacctgtggc
     1441 tgcgtcccgg tactgaggtg atcaagaagc acggcgacct gcatgacttt atgcagtggc
     1501 aagggccgat cctcaccgac tccggcggct tccaggtgtt cagcctcggc gcgatgcgca
     1561 agatcaagga ggagggcgtg tacttcgctt caccggtcga tggcgccaag gtgtttatgg
     1621 ggccggaaga gtcgatgcag gtgcagcgcg atctgggttc cgacatcgtg atgattttcg
     1681 acgagtgcac gccgtatccg gccgagttcg atgtggccaa acgctcgatg gagctgtcgc
     1741 tgcgctgggc caagcgttcg aaagaggctc atggcgaaag cacggcggcg ctgttcggca
     1801 tcgtccaggg cggcatgcat gaggaattgc gcaaagtctc tctggatggg cttgaagaga
     1861 ttggttttga cggctatgcc attggcggct tgtcggtcgg cgagccgaaa gacgaaatga
     1921 tcaaggtgct ggattacctg ccggcgcagc tgccggccga taaaccgcgt tacctgatgg
     1981 gcgtgggcaa gccggaagac ctggtcgaag gcgtgcgccg tggtgtggac atgtttgact
     2041 gcgtgatgcc gacccgtaac gcgcgcaacg gccacctgtt tgtcgacact ggcgtgctga
     2101 aaatccgcaa tgccttccat aaacacgacg attcgccgct ggactcgacg tgcgactgtt
     2161 acacctgcaa acatttctcc cgcgcctatc tgcaccatct ggataaatgt ggcgaaatgc
     2221 tcggtagcat gctcaataca atccacaact tacggcacta tcaggtgctt atggctggtt
     2281 tgcgcgaggc tattcaacag ggtacattgg ccgcctttgt cgatgccttc tatgccaaac
     2341 gcgggctggc aacgccgtct ctggattgat tcccaacctt ttaaagaccc ttgcaacagg
     2401 agtgttacat gagctttttt atccccgctg ctttcgctga aggcgccgca ccggctgccg
     2461 gtcctgctgg tagtggcttt gaatgggtat ttctggtcgg ctttctggtc atcttctatc
     2521 tgatgatctg gcgcccacag gccaagcgtg ccaaagagca caaaggtctg atcagcggcc
     2581 tgcagaaagg tgacgaagtg gtaaccagcg gcggtatcgc tggcaaggtg tcgaaggttt
     2641 ccgatgactt cgtggtaatc gaagtatccg acaccgtcga gctgaagatt cagaaagtgg
     2701 cgattgccgc ctcgctgcct aagggcacgc tgaaagcgat ctaagcaaca cacctttacc
     2761 attcgacggg gcgcttaatg cgccccgcgt cataacgggc ggcgtcatgc tcaacaaatt
     2821 tcccctctgg aagtacctgc tgattctggc tgtgctggca atcggcttcc tttattccgc
     2881 gcccaatctg tacccggatg atccggcgat ccagataagt ggcaacagct ccgccatgcg
     2941 cgtcgagcag gccgacctgg accgcgccag ccgcgccctg gaagcagccg gtatcagcgt
     3001 caaagctgtc agcctggcca atcagggccg tgctggcttg ctgcgtttga acagcgctga
     3061 agatcagctg ccggccaaag agctagtacg caaggccttg agcgacgagt ttgtggtggc
     3121 cttgaacctg gcgcaaacca cgccagattg gctgcgcaac ctgggcggta gcccgatgaa
     3181 gctcggtctg gacttgtccg gtggcgtgca cttcctgatg gaagtggaca tggacaaagc
     3241 cctggatgcg cgccgcaagg tttacgaagg cgaggtcaag agcctgctgc gtaaagagcg
     3301 cgtgcgttat cgcagcctgc ctgagctgga tggtgccatc cagctgggct ttgccgatga
     3361 agcggcgctg gaaaaagctc agcagctgat tcgcaaggac ttcaccgatt tcgacctgac
     3421 cactgtgcag cgtggcgagc agcaggtgct gcgcctggcg ctgactcagg ccaagctggt
     3481 cgaaattcgc gagtactcga tcaagcagaa cctgaccact gtacgtaacc gggtcaacga
     3541 gctgggcgtc gccgagccgc tggttcagcg tcagggtgcc aaccgcatcg tggttgagct
     3601 gccgggcgtg caggacacgg ccgaagctaa acgtattctc ggtaagaccg ccaacctgga
     3661 gttccgtctg gccgccgagc cggacgctgc caaggcggcg accgaaagct tcgaattccg
     3721 cgaggaaggc cgtccgccag cccaactgga gcgtgatctg atcatcaccg gtgaccaggt
     3781 gaccgatgct caggccagtt tcgatgagaa cggcagcccg caggtcaata tccgtctgga
     3841 tggccgcggt ggtgacctga tgaaccgcgc gacccgtaat aacgtcgggc gcagcatggc
     3901 ggtgatcttt atcgagcaga agccggtaac ccgctacgtg cgccaggtgg ttgatggcgt
     3961 cgagaaggaa gtcgagctgc agaccttcgt cgaagagaag aagatcatca gtctggccac
     4021 tattcagtcg ccgctgggta gccagttccg catcaccggc ctggatggtc agggcgagtc
     4081 gtccgagctg gccctgctgc tgcgtgcagg cggtctggct gcgccgatgt acttcgcgga
     4141 agagcgcacc attggcccga gcctgggtgc tgacaacatc gccaagggca tcagtgcgtc
     4201 gctgtgggcg atgctgtttg tttcgctgtt tatcatcgcc atctaccgct tcttcggtgt
     4261 attggcgacc atcgccctgg gcctgaacat ggtgctgttg ctggcgctga tgtcgttgct
     4321 cagtgccacc ctgaccctgc cgggtatcgc cggtatcgtg ctgaccatgg gtatggcggt
     4381 ggacgccaac gtgctgatct tctcgcgtat tcgtgaagag atcgccaacg gcatgtcgat
     4441 tcagcgagcc atccatgagg gttttgatcg ggcttactcg gcgattgtcg atgccaacct
     4501 gaccaccctg ctggtcggcg gcatcctgtt cgccatgggt accgggccgg tcaaaggctt
     4561 cgcggtgact ctgtcgctgg gtattctcac ctcgatgttt accgccattc tggtgacgcg
     4621 ttgcatggtc aacctgactt gcggcgggcg tgacttcaag aagttgtgga tttaaggggc
     4681 tgtgatgaat cgtacgatca acttcatggg ggtacgcaat attgcgttcg ccttcaccct
     4741 gctgctgacg ttgctggcac tggtt
//
LOCUS       DASRUM010000147         7772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_587137, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000147 DASRUM010000000
VERSION     DASRUM010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587137"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VFF22_09700"
     CDS             <1..461
                     /locus_tag="VFF22_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug transporter subunit MdtA"
                     /protein_id="HZX17121.1"
                     /translation="WDRSEKLKLAEGELESVDNLIDTATGTVKLKARFANAEEMLFPN
                     QFVNVRLRVKTLQNVTLMPAAALQFGASGTFAYVVDAEDKVQLTPISVGASDGLLSVV
                     EQGLNPGDRLVLEGTDRLRSGNKVEVIGDKPVAAQTEPATTERKRAPRAQ"
     gene            458..3550
                     /locus_tag="VFF22_09705"
     CDS             458..3550
                     /locus_tag="VFF22_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtB/MuxB family multidrug efflux RND
                     transporter permease subunit"
                     /protein_id="HZX17122.1"
                     /translation="MNASRLFILRPVATTLIMLAIFLSGLIAYRLLPVSALPEVDYPT
                     IRVMTLYPGASPDVMTSAVTAPLERQFGQMAGLKQMSSTSSGGASVITLRFNLEVQLD
                     VAEQEVQAAINGASNLLPSDLPAPPVYNKVNPADTPVLTLAISSKTMPLPQVFDLVDT
                     RLAQKLAQTSGVGLVTLAGGQRPAVRLRVNPEALASYGLNLSDVRTLITASNVNQPKG
                     NFDGPTRVSQLDANDQLKSVDEYRELILGYKDGATLRLKDVAEIIDGAENQRLAAWAN
                     RNEAVLVNVQRQPGANVIDVVDSIQTLLPNLTQGLPASVEVSVLTDRTETIRAAVKGV
                     QHELLLAIALVVLVTFLFLRKVSATIIPSIVVPLSLIGTFGVMYLAGFTINNLTLMAL
                     TIATGFVVDDAIVMLENIARHLEEGETPLNAALKGARQIGFTLVSLTISLIAVLIPLL
                     FMADVVGRLFREFAITLAVAILISLVVSLTLTPMMCARLLKAEKPEEEGRFYCAAGAV
                     IDGMIARYGVWLTWVLKHQPLTLLVAIGTLALTVLLYLAVPKGFFPVQDTGVIQGISE
                     APQSISFRAMSERQQRLADVILQDPAVASLSSYIGVDGDNPTLNSGRLLINLTPRAER
                     DVSASQIIERLRPELARIPGIELYLQPVQDLSIEDRVSRTQFQFSMESPDISLLEEWV
                     PKLVSALREQPELTDVASDLQNRGLQVFLQIDRDMAGRLGVSIADIDNALYDAFGQRQ
                     ISTIYTQASQYRVVLESENAGSIGPAALRQIHVKAADGQQVALSTLAQVEERAASLLI
                     NHIGQFPAATLSFNLASGVSLGEAVEVIERIEHEIGIPPAVQTRFQGAAEAFRASLSS
                     TLLLILAAIVTMYIVLGVLYESYIHPITILSTLPSAGVGALLALLISGNELGLIAIIG
                     IILLIGIVKKNAIMMIDFALDAERNQGMQPEAAIYQAALLRFRPILMTTLAALFGAIP
                     LMLATGSGAELRQPLGLVMVGGLLLSQILTLFTTPVIYLYFDRLSRRISGRSAAGVLV
                     "
     gene            3678..6785
                     /locus_tag="VFF22_09710"
     CDS             3678..6785
                     /locus_tag="VFF22_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HZX17123.1"
                     /translation="MNLSGPFIRRPVATLLLSLAILLLGGVSFGLLPVAPLPQMDFPT
                     ITVQASLPGASPQIMASSVATPLERSLGTIAGISQMNSRSSQGNTRIMLQFDLDRDIN
                     AAAREVQAAINASRELLPSGMRSMPTYAKVNPSQAPIMVLSLTSDVLNKGQLYDVAST
                     ILAQKLAQVTGVGEVQIGGSSLPAVRVALEPRLLDQYGIALDEVRQSISAANAKRPKG
                     AVEDAERHWQVQASDQLEKAADYLPLIIRYQDGAAVRLGDVATVTDGVEDRYNSGFFN
                     DEQAVLLVINRQTGANIIETIAGIREQLPALRAVIPASIELEVAMDRSPVIRATLHEA
                     EQTLLIAVVLVILVVFAFLGRWRAALIPALAVPVSLVGTFAVMYLLDFSLNNLSLMAL
                     IIATGLVVDDAIVVLENISRHIEAGETPMAAAFKGAREVGFTLLAMNLSLVAVFISIL
                     FMGGIIERLFREFSITLAVAIVISLLVSLTLTPVLCARWLKAETAEHKPSAMQRWGGA
                     VQTRVLAFYARGLDWALKHSLLMLFSLLATIALNIFLFVSVPKTFMPQQDTGQLMGMI
                     RGDDGLSFQVMQPKMEIFRRAVLADPAVDSVARFIGGSGGINNAFMIVRLKPVSERKA
                     SAQDVINRLRQSVPKVPGGRMFLMPDQDLQIGGREGRSSENEYMLLSGDLDELRKWLP
                     PVREALRALPELTDIDAKEAEGTQQIRLVVDRDMAKRLGVDMNMITAVLNNAFSQRQV
                     STIYDSLNQYSVVMEINPQFAQYPETLDQIQLITAEGARVPLSSFARWERSLEEDRVH
                     HQGQFAAENIGFALAEGVSLDQASQAINDAVARVGLPTEVQGMLGGTGGAFQQTKQNQ
                     PWMILLALVVVYIVLGVLYESYIHPLTILSTLPSAGVGALLALQLMSIEFSLISLLGL
                     FLLIGIVKKNAILMIDLALQLERQQHLSPEESIRQACLLRFRPIMMTTLAAILGALPL
                     MLSSAEGSEMRQPLGITIVGGLVLSQLLTLYTTPVIYLYFDRLRHRVNRWRGVRTDAA
                     LETPL"
     gene            6913..>7772
                     /locus_tag="VFF22_09715"
     CDS             6913..>7772
                     /locus_tag="VFF22_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZX17124.1"
                     /translation="MKALVAYRLPTLLALSLALSACAIGPDYQRPQLQTPAQFKQIEG
                     WTQAKPSDVLERGNWWQLYGDAELNALVERLNISNQNLAASEAQYRQARALVRGARAA
                     FYPSLSSSAGASRSGQGTGSQSTSNGTSSNNSGVSTSYDLSLNAAWELDIWGKLRRSL
                     ESSRAGFEASAADLAALKLSLQAELVQNYLQLRVLDDQQRLLDATVAAYARSLKLTEN
                     QYNAGIVPKSDVSQALTQLKSTQAQAIDLKWQRAQLEHAIAVLVGVPPSELSIAVREQ
                     LPALPEIPVAL"
BASE COUNT         1415 a         2459 c         2427 g         1471 t
ORIGIN      
        1 cctgggaccg cagtgaaaaa ctcaaactgg ccgaaggcga gctggagagc gtcgacaacc
       61 tgatcgacac cgctaccggt acggtcaagc tcaaggcgcg cttcgccaat gccgaagaaa
      121 tgctgtttcc caaccagttc gtaaacgtgc gcctgcgggt gaaaaccctg cagaacgtca
      181 ccctgatgcc ggccgccgcg ctgcaattcg gcgccagcgg caccttcgcc tatgtggtgg
      241 acgctgaaga taaggtgcag ctgacgccga tcagcgttgg cgccagcgac ggcctgctca
      301 gtgtggtcga gcagggcctc aatccgggtg atcgcctggt gctggaaggc accgaccgac
      361 tgcgctccgg aaacaaggtc gaagtgatcg gcgacaaacc ggtcgccgcc caaactgagc
      421 cagccacgac tgagcgcaaa cgagcgccgc gcgcccaatg aacgcctctc gcctgtttat
      481 cctgcgcccg gtcgccacca cgctgatcat gctggcgatc ttcctgagcg gcctgatcgc
      541 ctatcgtctg ctgcccgtgt cggcactgcc ggaagtggat tacccgacca tccgggtaat
      601 gaccctgtat cccggcgcga gcccggatgt gatgaccagt gcggtcaccg cgccgctgga
      661 gcgtcagttc ggccagatgg ccgggctgaa acaaatgtct tcgaccagtt cgggcggcgc
      721 ctcggtaatc accctgcgct ttaacctgga agtacagctg gatgtcgccg aacaggaagt
      781 gcaggcggcg atcaatggcg caagcaacct actgcccagc gatctgccgg caccgccggt
      841 gtacaacaag gtcaacccgg ccgatacccc ggtactgacc ctggccatca gctcgaaaac
      901 tatgccactg ccgcaggtgt tcgatctggt cgatacccgc ctggcgcaga agctcgcgca
      961 aaccagcggc gtcggccttg ttaccctggc tggcggccag cggccggccg tgcgtttgcg
     1021 ggtcaacccg gaagcgctgg cttcctatgg tttgaacctc agcgatgtgc gcacgctaat
     1081 caccgccagc aacgtcaatc agcccaaggg caacttcgac ggtccgaccc gggtgtcgca
     1141 gcttgatgcc aatgatcagc tgaagtccgt cgatgaatac cgcgagctga tcctgggtta
     1201 caaggacggc gcgacgctgc gcctgaagga tgtcgcagag atcatcgacg gtgcagaaaa
     1261 ccagcgtctg gccgcctggg ccaatcgcaa cgaagcggtg ctggtcaacg tgcagcgcca
     1321 acccggtgcc aacgtgattg atgtggtcga cagtattcag accctgctgc ccaacctgac
     1381 ccagggtcta ccggccagcg tcgaggtcag cgtgctgacc gaccgcaccg aaaccatccg
     1441 cgcggcggtc aagggcgtgc agcatgagct gttgctggcc atcgccctgg tggtgctggt
     1501 gaccttcctg tttctgcgca aggtttcagc gaccatcatc ccgtccattg tcgtgccgct
     1561 gtcgctgatc ggcaccttcg gcgtgatgta cctggccggc tttaccatta acaacctgac
     1621 gttgatggcc ctgaccatcg ccaccggctt tgtggtggac gatgccatcg tcatgctgga
     1681 gaacatcgcc cgccatctgg aagagggtga aacgccgctg aatgctgcac tgaaaggcgc
     1741 caggcagatc ggctttaccc tggtgtcgct gaccatttcg ctgatcgcgg tactgattcc
     1801 gctgctgttt atggccgacg tagtggggcg gctgttccgc gaattcgcca tcaccctggc
     1861 ggtggccatt ctgatttccc tggtggtgtc gctgaccttg acgccgatga tgtgcgcgcg
     1921 cctgctcaag gcggaaaagc ccgaggaaga agggcgcttc tactgcgccg ccggggcggt
     1981 gatcgacggc atgatcgccc gttacggcgt gtggctgacc tgggtactca agcatcagcc
     2041 gctgaccctg ctggtggcca tcggcaccct ggcgctgacc gtgctgttgt acctggcggt
     2101 gcccaagggc ttctttccgg tgcaggacac cggggtgatc cagggcattt ccgaagcgcc
     2161 gcagtcgatt tccttccgcg ccatgagcga gcgtcagcag cgcctggccg atgtgattct
     2221 gcaggacccg gccgtggcca gcctgtcgtc ctatatcggc gtggacggcg acaacccgac
     2281 gctgaacagc ggccgcctgc tgatcaacct cacgccacgg gccgagcgcg atgtcagcgc
     2341 cagccagatc atcgaacggc tgcgcccgga actggcgcgg attcctggaa tcgagctgta
     2401 cctgcagccg gtgcaggatc tgtctatcga ggatcgggtc agccgcacgc agttccagtt
     2461 cagcatggag tcgccggata tcagcctgct ggaagagtgg gtgcccaagc tggtcagtgc
     2521 gctgcgcgag cagccggaat tgacagacgt ggccagtgat ctgcaaaacc gtggcctgca
     2581 agtgtttctg cagatcgatc gcgatatggc cgggcgcctc ggcgtaagca ttgccgatat
     2641 cgacaacgcg ctgtacgacg ccttcggcca gcggcagatt tctaccatct acacccaggc
     2701 cagccagtac cgcgtggtgc tggaaagcga aaacgctggc agcatcggcc cagcggcgct
     2761 gcggcagatt cacgtcaagg ccgccgatgg tcagcaggtt gcactgtcga ccctagctca
     2821 agtggaagag cgcgccgcca gcctgctgat caaccatatc ggccaattcc cggcggccac
     2881 gctgtcattc aacctggcca gcggcgtatc gctgggcgag gcggtagagg tgattgagcg
     2941 gatcgagcat gagatcggca ttccgcctgc ggtgcagacg cgcttccagg gcgcggcaga
     3001 agccttccgt gcctcgctgt cgagcacctt gctgctgatc ctcgcggcca tcgtcaccat
     3061 gtatatcgtg ctcggtgtgc tctacgagag ctatatacac ccgatcacca ttctctccac
     3121 cttgccctcg gcgggcgttg gcgcgctgct ggcgttgctg atcagcggca acgagctggg
     3181 gctgatcgcg atcatcggca ttatcttgct gatcggcatc gtcaagaaga acgcgatcat
     3241 gatgatcgac ttcgccctcg acgccgaacg caaccagggc atgcagccgg aagcggcgat
     3301 ctaccaggcg gcgctgctgc gctttcggcc gattctgatg accaccttgg ccgcgctgtt
     3361 tggcgcgata ccgctgatgc tcgccaccgg ctccggcgct gaactgcgtc aaccgctggg
     3421 cctggtgatg gtcggcgggc tgctgctgag tcagatcctc acgctgttca ctacgccggt
     3481 gatctacctg tactttgatc gcctgtcgcg acggatcagt ggccgtagcg cggccggggt
     3541 tttggtatga gccgtagcgt gcatttgctc gccttgccga tttatcgggg agaatgtggc
     3601 tggccgcttc ccctcaccat aaccctctcc cctgagggga gaggggactg tccgcggttt
     3661 tctcgggttc gttcgctgtg aacctctccg gcccctttat ccgccggccg gtggccaccc
     3721 tgctgctgag cttggcgatt ctgctgctcg gcggcgtcag cttcggtctg ctgccggtgg
     3781 cgccgctgcc gcagatggat ttccccacca tcaccgtgca ggccagcctg ccgggggcca
     3841 gcccgcagat catggcttcc agcgtggcca cgccgctgga gcgctcgctc ggcaccatcg
     3901 ctggcatcag ccagatgaac agccgcagca gccagggcaa cacgcggatc atgctgcagt
     3961 tcgacctgga ccgagacatc aacgccgccg cccgcgaggt gcaggcggcg atcaatgcct
     4021 cgcgcgagct gctgcccagc ggcatgcgca gcatgccgac ctacgccaag gtcaacccgt
     4081 cgcaggcgcc gatcatggtg ctgtcgctga ccagcgatgt gctcaataag ggccagctgt
     4141 acgacgtggc ctcgaccatc ctggcgcaga aactcgccca ggtaaccggc gtcggcgaag
     4201 tgcagatcgg cggcagctcc ttgcccgccg tgcgcgtggc cctggagccg cgtctgctcg
     4261 atcagtacgg catcgccctg gatgaagtgc gccaaagcat tagcgccgcc aacgccaaac
     4321 ggccgaaagg cgcggtggaa gatgccgagc ggcactggca agtgcaggcc agcgatcagc
     4381 tggaaaaagc cgccgattac ctgccgctga ttattcgcta ccaggacggc gctgccgtac
     4441 gcctcggcga tgtggcgacc gtcaccgatg gcgtcgagga ccgctacaac agcggcttct
     4501 tcaacgatga gcaggcggtg ctgctggtga ttaaccggca gaccggggcc aatatcatcg
     4561 aaaccatcgc cggtattcgc gagcagctgc cggccttacg tgcggtgatt cccgccagca
     4621 tcgagctgga agtggcgatg gaccgctccc cagtgattcg cgccaccctg cacgaggccg
     4681 agcagaccct gctgatcgcc gtggtgctgg tgattctggt ggtgttcgcc tttctcggcc
     4741 gttggcgcgc ggcgctgatt ccggcgctgg cggtgccagt atcgttggtc ggcacctttg
     4801 cggtgatgta cctgctggat ttctccctga acaacctgtc gctgatggcg ctgatcatcg
     4861 ccacaggcct ggtggtagac gatgccatcg tcgttttgga aaacatttcg cggcatatcg
     4921 aggccggtga aacgcccatg gcggcggcgt tcaagggcgc gcgcgaggtg ggcttcacct
     4981 tgctggcgat gaacctgtcg ctggtcgcgg tgtttatctc gatcctgttt atgggtggca
     5041 tcatcgagcg gctgttccgc gagttttcca tcaccctggc ggtggccatc gtgatttccc
     5101 tgctggtgtc gctgaccctg acaccggtgc tctgcgcacg ctggctcaag gccgaaactg
     5161 ccgagcataa gcccagtgcc atgcagcgtt ggggcggggc cgtgcagacg cgcgtgctgg
     5221 cgttttacgc gcgcggtttg gattgggcgc taaagcactc cttgctgatg ctgttcagcc
     5281 tgctggccac cattgcgctg aatatctttc tgtttgtcag cgtgccgaag acctttatgc
     5341 cgcagcagga caccggccag ctgatgggca tgatccgcgg cgatgacggc ctgtcgtttc
     5401 aggtgatgca accgaagatg gaaatcttcc gccgcgcggt gctggctgac ccagcggtag
     5461 acagcgtggc caggtttatc ggcggcagcg gcgggatcaa caacgccttt atgatcgtgc
     5521 gcctcaagcc ggtatccgag cgcaaggcca gcgcccagga tgtgatcaac cgcctgcgcc
     5581 agtcggtgcc aaaagtgcct ggcgggcgca tgtttctaat gcccgatcag gacctgcaga
     5641 ttggcgggcg ggaagggcgc agctcggaaa acgaatacat gctgctgtcc ggtgacctgg
     5701 acgaattgcg caaatggctg ccgccggtgc gcgaagcgct gcgcgccctg ccggagctga
     5761 ccgatatcga cgccaaggaa gccgaaggca cccagcagat ccgcctggtg gtcgaccgcg
     5821 acatggccaa acggctgggc gtggatatga acatgatcac cgcggtgctg aataacgcct
     5881 ttagccagcg tcaggtgtcg actatctacg acagcctcaa tcagtacagc gtggtgatgg
     5941 agatcaaccc gcagttcgcc cagtacccgg aaaccctcga ccagattcag ctgatcactg
     6001 ccgaaggcgc gcgggtgccg ttgtccagtt tcgcccgctg ggagcgcagc ctggaagaag
     6061 accgagtgca tcaccagggc cagttcgccg cagaaaacat cggcttcgcc ctggccgaag
     6121 gcgtcagcct ggatcaggcc agccaggcga tcaacgacgc ggttgcccga gttggtttgc
     6181 ccaccgaagt gcagggcatg ctcggcggca ccggcggtgc cttccagcag actaagcaga
     6241 accagccatg gatgattctg ctggccctgg tggtggtgta tatcgtgctt ggcgtgctgt
     6301 acgagagcta tatccacccg ctgaccattc tctccacctt gccctcggcc ggggtcggcg
     6361 cgctgcttgc gctgcagctg atgagcatcg aattcagcct gatttcactg ctcggtctgt
     6421 ttttgctgat cggcatcgtc aagaagaacg ccattctgat gatcgacctg gcgctgcagc
     6481 tggaacgcca gcagcacctg agccccgaag aatcgatccg ccaggcctgc ctgctgcgct
     6541 tcaggccaat catgatgacc accctggcgg cgattctcgg cgcgctgccg ctgatgctca
     6601 gcagcgccga aggctcggaa atgcgccagc cgctgggtat caccattgtc ggcggattgg
     6661 tcctgagcca actgctgacc ctctacacca cgccggtgat ctacctgtat ttcgaccgcc
     6721 tgcgccaccg ggttaaccgt tggcgcggtg tgcgcactga cgctgctctg gaaactccgt
     6781 tatgagaggg gccattgcac ttcgtagggt gcgccgtgcg caccatgggt ttatggcggg
     6841 cgctggtgcg cacggcgcac cctacctaat tgtccaaacg ccttatgcat cacctgattg
     6901 gtacatcgcc ttatgaaagc cctagttgcc taccgtttac ctaccttgct ggcgttgagc
     6961 ctggctttga gtgcctgcgc cattggcccg gattaccagc gcccgcagct gcaaacccca
     7021 gcgcaattca aacagattga aggctggacc caggccaagc ccagcgatgt actggaacgc
     7081 ggcaactggt ggcagttgta tggcgatgct gagctgaatg ccctagtcga acggctgaat
     7141 atctccaacc agaacctggc cgccagcgag gcgcaatatc ggcaggcccg cgctttggtg
     7201 cgcggtgcgc gagcggcgtt ctatccgagc ctgtcgagta gcgctggggc cagccgtagc
     7261 gggcagggca cagggagtca gagtacgtcc aacggcacca gctcgaacaa cagcggcgtg
     7321 tccaccagct acgacctcag cctgaatgcc gcctgggagc tggatatctg gggcaagctg
     7381 cgccgctcgc tggagtccag ccgtgcaggc ttcgaggcca gcgccgccga cctggccgcg
     7441 ctgaaactca gcctgcaggc agaactggtg cagaactacc tgcaactgcg cgtgctggat
     7501 gatcagcagc gcctactgga tgccaccgtc gcggcctacg cccgttcgct gaagctcacg
     7561 gaaaaccagt acaacgccgg catcgtgccg aaatccgatg tcagccaggc gctgacccag
     7621 ctgaaaagca cccaggccca ggccatcgac ctgaaatggc agcgcgccca gctggaacac
     7681 gcgattgccg tgctggtcgg cgtaccaccc agcgaactca gcattgccgt gcgtgagcaa
     7741 ttgcccgcgc tgccggaaat ccccgtggcc ct
//
LOCUS       DASRUM010000148        29719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1254516, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000148 DASRUM010000000
VERSION     DASRUM010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 29719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29719
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254516"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /gene="ssrA"
                     /locus_tag="VFF22_09720"
     tmRNA           <1..140
                     /gene="ssrA"
                     /locus_tag="VFF22_09720"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(240..914)
                     /locus_tag="VFF22_09725"
     CDS             complement(240..914)
                     /locus_tag="VFF22_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008015172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HZX17125.1"
                     /translation="MRTAFFVDGYNLYYGVLQGSDHKWLDLSALLAEILRIQDPAAEL
                     VSVDYFTSPVITNLATRGIVSNDAQSRYIRALQAKGVSVTLGRHRLARGYAPRYLDGH
                     KPSRKDQVAIWELEEKETDVNLALGMYRAACRAVDLQLNQIVLVSGDTDLAPALAAIR
                     SDFPHIRIGVILPHRQEQRNPPGSLKEHAHWMRRHIPESELAAFQLPPKVPLPRKPAI
                     VKPDYW"
     gene            complement(1219..2220)
                     /locus_tag="VFF22_09730"
     CDS             complement(1219..2220)
                     /locus_tag="VFF22_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZX17126.1"
                     /translation="MSVKRRDDGRWLADVQPIRGQRFRKLFDTKAEAQRYETFILANK
                     AKDAGWNPKASDRRKLSELVTLWYDLHGHMLKDGLRRKARLDAICVRLRNPVAAKLDA
                     SVYAHDRRVRSENGTSPKTLNNELGYLRAVFNELRGLGQIDYANPLELVKPFRIDERE
                     LSWLTDDQVSILLDAIRKHSDNPHVEPITLLCLATGARWSEAEGLIPARLRNGSVTYS
                     KTKSSRVRTVPIDPALETFIREHWKRHGPFTGSISSFRRALVRSGIQLPKGQASHALR
                     HTFASHFIQKGGNILTLQKILGHSSLAMTMRYSHLAPEHLAEAVRLSPLAGHLWGRL"
     gene            complement(2217..3500)
                     /locus_tag="VFF22_09735"
     CDS             complement(2217..3500)
                     /locus_tag="VFF22_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17127.1"
                     /translation="MATVKDHQRLNALTAEDDKHGRLFVDPGNVTFTDLSGVRLLRCG
                     VDTVRQLYKGLIRPEIMALFEVPGTLVDFAGQRWHSGRVGRDSGYQYKLQNADLGIIL
                     LVKNFNAKLDCIGPHLKIEVSPHAIDSLSPERLQDRLDFYASQVMTHVEINQCAVHLA
                     LDLQGWTPPADLVARMHCRATTHRDISGINEINWATKSSTYGRGETSMFGSASGIQLC
                     IYNKTEQAKATDKLDYWESVWRRRDSFDAADPQNYNPEQPVWRVELRYHHSIIQQFAS
                     GSLDVKTGEAIDTRSFAAFSGHLDGLWRYGLGQFKLLSRPGYFAPIWTLIREDIRVDL
                     PVDSLLEDTEYKRYYKTSRGFSGKNVELFLGNFVSLLARERVGAKKAFDRLQTWECWP
                     VIRDHYAAKDMSEHDIYKHIKKLLEERSVRWGRAV"
     gene            complement(3912..4304)
                     /locus_tag="VFF22_09740"
     CDS             complement(3912..4304)
                     /locus_tag="VFF22_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17128.1"
                     /translation="MSDQNSPVDLATALTYLGKTALIEQGWRQEPDSFWQLMHIVGVV
                     LPMEGICKHGYFLVIEVSNTQAHPNEVFFTDIRTIRAIRHRDRQSSGNVLDRMTRPSS
                     LAVSRAALPARRNSSTVPSNGSTGAAHP"
     gene            complement(4297..4659)
                     /locus_tag="VFF22_09745"
     CDS             complement(4297..4659)
                     /locus_tag="VFF22_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17129.1"
                     /translation="MPSQRISLDAATAISYIGQPVLVELVWDGDPEPIWLFKHIVGVV
                     VPVQGVNDIGYFLTVPVIGSTDFPDETYFDSIRTIRTLPATRSRGQRTSGKVLSRLPL
                     PHLIQGNADLMEGGSSHV"
     gene            complement(4652..4864)
                     /locus_tag="VFF22_09750"
     CDS             complement(4652..4864)
                     /locus_tag="VFF22_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17130.1"
                     /translation="MISDDSLDLCSVKTFAEMSGISIEEAIDWVDNGTVPSMRLADFR
                     MVNLARLRADLLKGKKQFKAGDYRHA"
     gene            complement(4854..5024)
                     /locus_tag="VFF22_09755"
     CDS             complement(4854..5024)
                     /locus_tag="VFF22_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HZX17131.1"
                     /translation="MSRTDPQFKLRVPAALRLQIQQAAHATRRSMNAEMVIRLEASFA
                     KEQPLQEKTHDQ"
     gene            complement(5021..5242)
                     /locus_tag="VFF22_09760"
     CDS             complement(5021..5242)
                     /locus_tag="VFF22_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HZX17132.1"
                     /translation="MLPPEGLDPQKLHGAPPLMPWQKFAEWIGMGDEPVVVRTWVERN
                     YLPSQKLGKRTMVNVALLHQQLLEQEWTL"
     gene            complement(5785..6597)
                     /locus_tag="VFF22_09765"
     CDS             complement(5785..6597)
                     /locus_tag="VFF22_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17133.1"
                     /translation="MPQEFYEIIKYLVGLAAGAGLTIAMIAKFGEKIFFKHLDHKYSE
                     KLSQRNVELQSELEETKNSLNRSLQQDVASYKADLHVLSGQRARYLERKIDSILELNK
                     FYVFAAKALLEYISTNHAYIDEAAYYFIFQSEEEDFTSFSDYRIYAEIKREHWPKLLK
                     PAESAINQYSEALALRMPILPKEFSSSELALASSLQEIMDTSKGLFYRTMGLTSEIVE
                     PEEEMTPEECLAEFKVHVAKSLELKIKVESYYQDLLSKAQESNELIESLLIS"
     gene            complement(6771..7724)
                     /locus_tag="VFF22_09770"
     CDS             complement(6771..7724)
                     /locus_tag="VFF22_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17134.1"
                     /translation="MKNKITVSGSFVVLIGVIISLAVIFFKTIDVSIDLFQGVVYEFL
                     LAISIGLITMGILSVILGLKDWQVYFQQRLSEVIRQKEYIRSLNKDELVQLQRDTFRA
                     YFDIDLEGNQNNFLSFFENELQHLIGSPYREDINYFMDVKSVNGDGLEIEENIRFRAK
                     SVAGKIQDSINWVASREEYVSFDFGQVKIKPSKQFSGDVVVDDDGYYELNNNEMKLIE
                     GGLGFRASLEKFIDNEFVDVIISAKYKAHPKRYLSWTMSQLTDRFTITINYPENMNST
                     YEVLGINMDNVQVNRGRSIITIKCNTWALPYTGVVLGLDAA"
     gene            complement(8426..9331)
                     /locus_tag="VFF22_09775"
     CDS             complement(8426..9331)
                     /locus_tag="VFF22_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX17135.1"
                     /translation="MRQPNLTDVALFAAVVEAGGFRVAAQKRGMSASSISDGVRRLES
                     DLGVRLLNRTTRSVFPTEVGARLLERLRPALEEIATAFGDLDDNALSPVGTLRLNVPV
                     PVARFVLPKLIPPFLERCPGVSIEVTMDNTFIDVIAAGYDAGVRYEESLAKDMIAVPI
                     GPRRQRFVAAASPGYLQQNGTPLHPRDLLNHRLIGHRFESGKLGVWVFEKDGATIRVP
                     PQGPLLSSSQDLEVGAAVGGVGIIYTFEEYLGPLLKQGTLLPVLEDWWQAFDGPYLYY
                     NDRRHVPSPLRAFVDFLKAEAQQSF"
     gene            9586..10542
                     /locus_tag="VFF22_09780"
     CDS             9586..10542
                     /locus_tag="VFF22_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX17136.1"
                     /translation="MLEVGSVSAQSQPKPDWSLADIPSQSGRIFLVTGGTSGMGYEDA
                     KALAAAGAQVVIAARNPQRGEEAIARIKQEVADAQVQFEAVDLANLASVQALGERLNA
                     TLPRLDGLINNAAIMSPPQRGSSADGFESQLATNYLGHFALTAQLLPLLRKSDGARVV
                     TLSSIAAARGAINFDDLQSEQAYNPFGAYAQSKLATLMFAFELQRRSEAQGWGIQSIA
                     AHPGVAVTELIERGPGLNSEFGRNWAKDRERYHSAAQGALPTLYAATAPEAQPGAYYG
                     PIGEEEKRGPLGLASVPAAAQDAAAAARLWTVSEQLTGVSYR"
     gene            10565..11902
                     /locus_tag="VFF22_09785"
     CDS             10565..11902
                     /locus_tag="VFF22_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX17137.1"
                     /translation="MLSATLVQRLGAAINAERHELNPALAGFALFFCLFSGYFMLRPI
                     RESMGIMAGIENLQWLFTATFVVMLVAVPLFAWLSSRVPRIHFVDWVYGFFCLNLLAF
                     AGLFQLSADNVWLARVFYVWISVYNLFVVSVAWSLMADVFDGRQAKRLFAFIAAGASV
                     GGLVGPAMSALLVGVIGQAGLVLLAAVLLGVALALKAPLMRWREIGGAGRPGAPITES
                     THKPVTGNPFSGLTRVLQSPYLFAIAGFVVLLATVTTFLYFEQARLVAERFPDRESQI
                     RVFGIIDVIVQAGALLSQLFITGRIAQKLGVRMLLAIVPILVCVGFIGLALAPTFAML
                     AALMIVRRIGEYAFVRPGREMLFAPLDAESKYKAKGFIDTVVYRAGDAISGWLKSGLD
                     MLGQGAVLVALVGAACALIWGVLGWYLGRQADELSKCAAPEVSERDAALLAGQ"
     gene            complement(11914..14721)
                     /locus_tag="VFF22_09790"
     CDS             complement(11914..14721)
                     /locus_tag="VFF22_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004575777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HZX17138.1"
                     /translation="MSLPAAFLTAVERLIPAERRFDDPLSTLAFGTDASFYRLIPKLV
                     VRVEAELEVVDLLRLAGAHQVPVTFRAAGTSLSGQAISDSVLIVLGDNWNGREVRGQG
                     SQIRLQPGVIGAQANAVLAPFQRKIGPDPASINACKIGGIVANNASGMCCGTAQNSYH
                     TLAGIRLVLADGTVLDTEDPLSVTRFFATHADLLEALAELGRQTRANSTLAAKIRHKY
                     RLKNTTGLSLNALVDFDQPLDILSHLLVGSEGTLGFISAVTYNTVPDHPHKASALLVF
                     PDVESCCRAVTLLKQQPVSAVELLDRRSLRSVQDKPGMPEWVKGLSDSACALLIESRA
                     ASQSLLHEQLQLIMASIAHFPLEKQVDFSEDPAVYNLLWKIRKDTFPAVGAVRQTGTT
                     VIIEDVTFPIEQLAEGVNRLLALFDKHGYDEAIIFGHALEGNLHFVFTQAFETPEQIA
                     RYSAFMDDVAQLVAVEFGGSLKAEHGTGRNMAPFVELEWGTDAYQLMWQIKRLLDPQG
                     ILNPDVVLSDDPQIHLKNLKPLPAADAIVDKCIECGFCEPVCPSKGLTLSPRQRIVIW
                     RDIQAKKRAGIDTRELEAAYQYQGIDTCAATGLCAQRCPVGINTGDLVRQLRARDANS
                     QRSADWLANNFRRAMQGARVMLHVANGARLLMGTPLLSRTSAALSKASQGKLPQWTPA
                     MPQPLQRLHLPKTNDDARPRVVYLAACVSRAMGPAASDNEQVPLLDKTRALLEKGGFQ
                     VVFPADLDNLCCGQPFASKGYAEQAERKKQELIDALLKASRGGLDPIYCDTSPCTLRL
                     VQDGLDARLKLHDPVKFIREQLLDRLQITPQDKPVAVHVTCSTQHLGEAQGLIDIVRR
                     CTSEVIIPEGIHCCGFAGDKGFTTPELNRHALRSLKSAVQYCEEGISTSRTCEIGLSQ
                     HGDIDYHGLVYLLDRVSQARA"
     gene            complement(14759..15913)
                     /gene="lldD"
                     /locus_tag="VFF22_09795"
     CDS             complement(14759..15913)
                     /gene="lldD"
                     /locus_tag="VFF22_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004457 - lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent L-lactate dehydrogenase LldD"
                     /protein_id="HZX17139.1"
                     /translation="MIISASTDYRAAAQRRLPPFLFHYIDGGAYAEHTLKRNVADLAD
                     IALRQRVLRNMSELSLETRLLGETLSMPVALAPVGLTGMYARRGEVQAAKAAAAKGIP
                     FTLSTVSVCPIEEVAPAIDRPMWFQLYVLKDRGFMKNALARAKAAGVTTLVFTVDMPV
                     PGARYRDAHSGMSGPNAPLRRMLQAMTHPRWAWDVGLLGKPHDLGNISAYRGNPTGLA
                     DYIGWLGNNFDPSISWKDLEWIREFWDGPMVIKGILDPQDAKDAVSFGADGIVVSNHG
                     GRQLDGVMSSARALPAIADAVKGQLAILADSGIRTGLDVVRMIALGADTVMLGRAFIY
                     ALAADGGAGVSNLLDLIEKEMRVAMVLTGAKSISEITADSLVRELHHAAS"
     gene            complement(15994..17688)
                     /locus_tag="VFF22_09800"
     CDS             complement(15994..17688)
                     /locus_tag="VFF22_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HZX17140.1"
                     /translation="MSTGLLALLAFSPILLAAVLLIGLRWPAKHAMPLVYLLTAGVAL
                     FAWDMSFNRVLASTVQGLLITLGLLWIIFGAILLLNTLKHSGGITAIRAGFATISPDR
                     RIQAIIIAWLFGCFIEGASGFGTPAAIAAPLLVAIGFPAMAAVMLGMLVQSTPVSFGA
                     VGTPIIVGINTGLDSATIGAQLIEQGSSWAQFLQLITSEVAIIHAIVGTVMPLVMVLM
                     LTRFFGTEKSWKAGFEVLPFAIFAGLAFTIPYALTGVFLGPEFPSLGGGLIGLAIVTS
                     AARMGFLLPKTTWDFAPADKWPAEWLGTVEMKLDQLTAKPMSALRAWLPYVLVGVLLV
                     ISRVFPEVSTALKAVMLNFPDLLGETGIGANFQPLYLPGGILVAVVIATFFFHGMKLR
                     ELGSAVKESSSVLLSAGFVLLFTVPMVRILINSGVNAAELSSMPILMARWVADSVGGI
                     YPLLAPSVGALGAFIAGSNTVSNMMFSQFQFGVASSLGISSALVVAVQAIGAAAGNMV
                     AIHNVVAASATVGLLGREGSILRKTVWPTLYYVLFTGLIALFAVYVLGISDPLMAG"
     gene            18014..18784
                     /locus_tag="VFF22_09805"
     CDS             18014..18784
                     /locus_tag="VFF22_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HZX17141.1"
                     /translation="MEVGSVRQRRLSDDIVSQLETMILEGTLKAGERLPAERVLSEQF
                     GVSRPSLREAIQKLAAKGLLVSRHGGGNYVAETLGSTFSDPLLHLLESNPEAQRDLLE
                     FRHTLEGSCAFYAAQRATELDRQRLTDAFAVLQDCYSRSGKVTRAEEGAADANFHLAI
                     AEASHNAVLLHTIRGLFDLLKRNVVTNIGGMYAQRDETRDMLIEQHRTLYQAIIEGRA
                     EDARALSNRHIDYVQEVLAEVQQQARREERALRRGSTR"
     gene            complement(18792..20261)
                     /locus_tag="VFF22_09810"
     CDS             complement(18792..20261)
                     /locus_tag="VFF22_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX17142.1"
                     /translation="MPKVLRSIRARLLVLLLSLLSVTLGLISHKIYLDASHEVEELFD
                     AQLAQTSRMLMGLVRHELSADSRRDLQAALDEALLLKNSPRNLDGLLGHDYESKLAFQ
                     MLDDNGELVFQSASAPKGLLMEMIAQLGLNLPAGEQPLSERLNRLAKYLSGYHTLTLG
                     EHRWRVFVLHDNVDHHWVLVGEREDVRGELSGKIARRTLQPLLIGLPLLALLLWLAVG
                     FGLRPLQRMAESIKARAPDNLAPLTFGPLPQELEPMGASLNRLLMQVNQLLDQEKRFI
                     ADAAHELRTPLAVLRIHAQNALEAPDPQDRSDALRQLGSGVERATRVVAQLLTLARLE
                     PNVVQLAMGELDLLGFVRNELAELIPLALERHQELNLDAVEPADYRLLADGPSLGTLL
                     QNLISNAVQYTPDGGRIQVQLEAQVDAIMLRVQDSGPGVDEAQREKLFERFYRLGDGQ
                     GAGLGLSIVLRVVELHRASISLGDSPLGGLEVAVRLPRL"
     gene            complement(20263..20919)
                     /locus_tag="VFF22_09815"
     CDS             complement(20263..20919)
                     /locus_tag="VFF22_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZX17143.1"
                     /translation="MRILLVEDDKALGEGIRTALKPEGYTVDWVQDGSSALHALSSES
                     FELAILDLGLPRMDGLQVLKHLRAAANPVPVLVLTARDSTSDRIAGLDAGADDYLVKP
                     FDVAELKARLRALLRRSFNRPQPALEYRGISLDPVSQSVSYQGQAVNLPRKEFLLLHE
                     LLAQPGRVMTRDKLQQALYGWDEEPESNALEVHVHHLRKKFFAELIRTVRGVGYLVDK
                     "
     gene            complement(20964..21614)
                     /locus_tag="VFF22_09820"
     CDS             complement(20964..21614)
                     /locus_tag="VFF22_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZX17144.1"
                     /translation="MNLIRNAACALLCLVSLPSFALSEQGQAQLQSLQDRWAEINYQL
                     PKEQREKAFTSLSEQAQKAVAAEPNAAELQIWNGIILSTYAGAKGGLGALSLVKQAKA
                     SLEQGLALDPQALSGSAYTSLGSLYYQVPGWPVGFGDDEKAETMLKQALAINPDGIDP
                     NYFYGDYLARAKRFEEAKAALEKALQAPARPNRLSADAGRKEEIATLLSKVKAELD"
     gene            complement(21627..22418)
                     /locus_tag="VFF22_09825"
     CDS             complement(21627..22418)
                     /locus_tag="VFF22_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX17145.1"
                     /translation="MKLSECRAVITGASGGIGQALLKALLAEGAQLLLVGRQAEALQA
                     LAQAHPGKVAVVAADIRQRSGREAVLAAAQRFGGLNTLINAAGVNHFGLLEQHDESAI
                     AELIDLNVTATLQLTHRLLPLLRQQGEALVVNLGSTFGSIGYPGFSAYCASKFALRGF
                     SEALRRELADTPVKVLYFAPRATRTSMNAANVVAMNDELNVAMDEPQQVAQQLLQAIR
                     REDDERYLGWPERFFVRLNSLLPRVVDQALRKQLPIIQRFARSKA"
     gene            complement(22415..23080)
                     /locus_tag="VFF22_09830"
     CDS             complement(22415..23080)
                     /locus_tag="VFF22_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HZX17146.1"
                     /translation="MSFYESLLEQTNAERNYLLGAPIIAQAMAGSVSLNSYVAFLTEA
                     YHHVKHTVPLLMACGARLPERLEWLREAIAEYIDEEKGHQEWVLNDIAACSADKEAVR
                     NGTPRLPTELMVSYVYDRIARHNPVSFFGMVNVLEGTSIALATQVAGVIQDKLQLPNK
                     AFSYLSSHGSLDLEHIEFFKRLMNQLDNDDDKAAVVHTAKVVYRLYGDIFRSLPLSAQ
                     EPQ"
     gene            complement(23080..24540)
                     /locus_tag="VFF22_09835"
     CDS             complement(23080..24540)
                     /locus_tag="VFF22_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZX17147.1"
                     /translation="MWPAAKPLFHRLAALGDALALREGDRQLSYAQLLSEIEQRSTQL
                     QQLGARRVALALDNGIEWVLWDLATLHAGLICVPVPGFFSSGQQAHVLDSAGIDCLIS
                     VQPHASFVQALPGLHLRPMPQASAVHAGTCKITYTSGTTGQPKGVCLDIATQLAVAHS
                     LVQTSASCKVERHLCVLQLATLLENIAGVYAPLLAGAQIELMPMAQIGLLGASQFDLP
                     RFLGALAQAQPNSLILLPQLLLALVSAAERGLPVPNSLRFIAVGGGRVASQLLQRADA
                     LGLPVFEGYGLSECASVVCLNTPENRRIGTVGQPLPHLQVRLGVDQEVEVKGPRLLGY
                     LGEPSPAGEWLGTGDLGHFDGKFLVLHGRKKHQFITAYGRNVNPEWVEAELVQQLPIA
                     QAWLHGEALPGNVAVLVPRFPNTSDSQLAKAVATANQSLPDYARVHHWLRAAQPFSSA
                     NELATSNGRLRRNALLNHYQSAIAQLMASESCYGDA"
     gene            complement(24524..25213)
                     /locus_tag="VFF22_09840"
     CDS             complement(24524..25213)
                     /locus_tag="VFF22_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermostable hemolysin"
                     /protein_id="HZX17148.1"
                     /translation="MELPWVYHDTALAQIGREQPLSLYLANATSPRRAGIEAFIGERF
                     AAQHQARIRHFMPCLLSLEDSAGQLLGAVGLRSAASGPLFLERYLAQPAEQVITAQQV
                     ANAPERKQLVEVGNLAAGSPGAARLLIVALTDLLVALGYRWVCFTGTLALLNSFQRLG
                     LTPLPLGPASLDCMGDEQADWGTYYDNQPQVMAGDIYAGHQRLLQLGAYPRLAHQAFY
                     ALEDMPHVACR"
     gene            25427..25840
                     /locus_tag="VFF22_09845"
     CDS             25427..25840
                     /locus_tag="VFF22_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17149.1"
                     /translation="MPPMDAEQQLATRLAILENPEAQDCTVYRADDQDPEAEEVDLGD
                     AKVLFCGPFEAPADWDAAEREDYFGDSAPELFVNARIECEAKPGSKGHFTVDIGDYVA
                     AQPGLGEVVMFYVHDYLEDDSGCTYVLLRDEEVLE"
     gene            26017..26598
                     /locus_tag="VFF22_09850"
     CDS             26017..26598
                     /locus_tag="VFF22_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017479308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HZX17150.1"
                     /translation="MAAKKILMLVGDYVEDYEVMVPFQALLMVGHQVHAVCPDKTAGQ
                     SVRTAIHDFEGEQTYSEKPGHNFALNFDFAQVKAEDYDALLIPGGRAPEYLRLNAQVL
                     ALVQAFDKAGKPIAAVCHGAQLLAAAGVLKGRECSAYPACGPEVTLAGGRYIDIAVDQ
                     AHVQGNLVTAPAWPAHPNWLAAFLGLLGTQITL"
     gene            26645..26947
                     /locus_tag="VFF22_09855"
     CDS             26645..26947
                     /locus_tag="VFF22_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016982789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HZX17151.1"
                     /translation="MCELYVKADPILYESRARSLRIRGVVTTLRLENQFWDILREIAE
                     VDGMTTNQLITKLYDEVMDYRGEVVNFASFLRVSCTRYLSQRRAPAELSLVRAIQS"
     gene            complement(27128..28138)
                     /locus_tag="VFF22_09860"
     CDS             complement(27128..28138)
                     /locus_tag="VFF22_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010449306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4917 family protein"
                     /protein_id="HZX17152.1"
                     /translation="MQTPAFTGELEDWNTLNSSIGFPELLVGNGASLAIWRKFAYFSL
                     FDEAQTTRNRPLSATELSVFRALDTSNFEQVLSALKNSIRVNAALTINSSSPRHRYFA
                     IKEALIHAVRAVHIPWSQMQADSLTRINKALSQYQTVYSGNYDLLNYWAVMQAPEAFE
                     DMFRGADSTFAPGDCQRQGNATRMLYLHGGMHLVKNIDGSTRILNSSESTLLASFAIN
                     QLGDIPLFVNEGSSADKLKAIRSSDYLSFCYSQLAQPKDSLCIFGHSLAAQDQHLIQA
                     LQQAELKTLAISIYPHNPLFITQQKQHYASLFAEQNVELRFFDALSHPLGDPALRISA
                     TA"
     gene            complement(28144..29070)
                     /locus_tag="VFF22_09865"
     CDS             complement(28144..29070)
                     /locus_tag="VFF22_09865"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZX17153.1"
                     /translation="MDSSASRSTWLDVLITAMAPAIWGSTYIVTTEILPPDRPFTAAL
                     LRALPAGLLLVLYSRYLPRRSEWPRLLVLAALNIGFFQALLFVAAYRLPGGLAAVVGA
                     IQPLLVMGLVWSMDQQRPASVAVAASVVGIAGMAALLLAPGASWDPIGIAAAFVGTAC
                     MASGTYLTRRWRPTVPLLAFTGWQLLIGGLFLLPVAWLIDPPLPQLSPLQWLGYAYLS
                     LFGALLAYVLWFRGIARLAPVAVSSLGLLSPLVAVLLGWALLGQSITGVALLGLIAVL
                     GSILAVQWAAAAKPAVVAPMLKSHSDTAQGDN"
     gene            29237..29665
                     /locus_tag="VFF22_09870"
     CDS             29237..29665
                     /locus_tag="VFF22_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZX17154.1"
                     /translation="MGVIGRLGRCASLLRRELDKLFSNFGLTSWEFDVLATLRRSGEP
                     YRLAPTTLFSTLMVTSGTMTRQLQQLEGSGLVSRIANPSDARSMLVQLTPAGLELIDR
                     AVTAHVANEARFLAPIAATELAQLEARLSELLAVLEPAGE"
BASE COUNT         6019 a         8587 c         9143 g         5970 t
ORIGIN      
        1 agcggctaaa acttacacaa ctcgtccaaa gcaccctgcc cgtcgggcgg ctgcggatta
       61 actcaataga cacggctaag catgtagtac cgaaagtgga gaactggcgg acgggggttc
      121 aaatcccccc ggctccacca attagaaaca ccttaagccc ctgattttcc tagagaaagt
      181 caggggtttt tgctttcgac acttgtcgac tgtcccgaaa gtgtcacacc tgcaaactgc
      241 taccagtaat caggctttac gatggctggc tttctgggaa gcgggacttt tggcgggagc
      301 tggaaagcgg caagctccga ctcaggaatg tgacggcgca tccagtgagc atgctctttc
      361 aatgaccccg gcggattccg ctgttcctga cggtgcggca gaatcacgcc aatgcggata
      421 tgtggaaaat ctgaacgtat ggcagcgagc gcaggggcaa ggtcagtatc gcccgaaacc
      481 agaacgatct gattgagctg cagatccaca gcacgacatg ctgcacggta catgcccaat
      541 gccagattga cgtccgtctc tttctcttca agctcccaaa tcgccacctg atccttcctg
      601 gatggcttat gaccatcgag atacctgggc gcataccccc tggccaaacg gtgcctacca
      661 agcgtgaccg atacgccctt cgcctgcaaa gctcgaatgt agcggctttg agcatcgttg
      721 gagacgatcc cacgagtagc aagattggta attacgggag aggtgaagta atcaacggag
      781 accagctctg ctgcaggatc ctgaatgcgc aagatctcgg ccagcaacgc agacagatcc
      841 aaccacttgt ggtcagaacc ttgaagcaca ccataataga ggttgtagcc gtctacgaaa
      901 aaagctgtac gcacaaatcc tgatccctag aaacgaaaaa accggcgatt aggccggttt
      961 cttcacccta ggctaaagct gaacttaatc ggcccgccat gggttgagtc gtgagccaaa
     1021 tattactcta atttgcgtaa tgtgcaaatt gtgtgtcgtg cataaaatgc gtgtcatgta
     1081 caaattgtgt gtcatgcgca aaatgcgtgt catgtacaaa ttgcgtgtcg tgcacaaaat
     1141 gcgggccgtg cacaaattgt gcgtaatgaa attactgccc acaccgcaga aaatgtgaaa
     1201 acacgaaaag ccagccaatc acagacggcc ccacagatgt cctgccaacg gactcagcct
     1261 caccgcctcc gccagatgct ctggagccag atgagagtag cgcattgtca tagccaggct
     1321 ggaatgacct aggatctttt gcagggtcag gatgttgccg cccttctgta tgaagtgact
     1381 ggcgaaggtg tggcgtagcg cgtgactggc ctggcctttc ggtagctgga ttcctgaccg
     1441 aaccagggca cgacgaaatg agctgattga gccagtgaac gggccatgac gcttccagtg
     1501 ttcccttatg aatgtctcca gggccggatc gattggcact gtgcggacgc ggctggattt
     1561 ggtcttactg taagtcaccg agccattacg caaccgggcc gggattaacc cttctgcctc
     1621 agaccaacgc gcaccagttg ccaggcatag cagggtgatc ggttcaacat gcgggttgtc
     1681 cgagtgcttc cggatagcgt ccagcagaat gcttacctga tcatctgtga gccaggacag
     1741 ttcacgctca tcaatgcgga atggcttcac cagctccaat gggttggcgt agtcgatctg
     1801 gccaaggccg cgaagctcat tgaacaccgc acgcaggtag cccaactcat tgttgagggt
     1861 cttaggactg gtgccgtttt cagagcgaac gcgccgatca tgggcataaa ccgaagcatc
     1921 gagcttggca gctaccgggt tacggagccg aacgcatata gcatcgagcc tggcctttcg
     1981 acgtagacca tccttgagca tgtgaccgtg caggtcatac cagagcgtca ccagttccga
     2041 gagtttgcga cgatctgacg ccttggggtt ccaaccggca tccttggctt tgttggccag
     2101 aatgaacgtc tcataacgct gggcttcagc cttggtatca aagagcttgc ggaagcgttg
     2161 accacggatc ggctgaacat ctgccaacca gcgtccatca tcgcgccgct tcacactcat
     2221 acagcacgac cccaccgaac gctgcgttct tccagcagct tcttgatgtg cttgtatatg
     2281 tcgtgctcgc tcatgtcctt ggcggcgtag tgatcgcgaa tgaccggcca gcattcccag
     2341 gtttgcagcc gatcaaacgc cttttttgcg cccacccgct cccttgccag caggcttacg
     2401 aagtttccca ggaagagctc cacgttcttg cccgagaagc cacgggaggt cttgtagtag
     2461 cgcttgtatt cagtgtcctc aagcagggaa tcaacgggta ggtccacacg tatgtcctcg
     2521 cgaataagtg tccagatggg ggcgaagtag cccggtcggc tcaacaactt gaattgcccc
     2581 aacccatagc gccacaggcc gtccagatgc ccggagaagg ccgcaaagga acgggtgtcg
     2641 atggcttcac cggtcttcac atccagggag ccgctggcaa attgctggat gatcgaatgg
     2701 tgataacgca gctctacacg ccataccggt tgctcggggt tgtagttctg cgggtctgca
     2761 gcatcgaagc tgtcacgacg acgccagacg ctttcccagt agtcgagctt gtccgtggcc
     2821 ttggcctgct cggtcttgtt gtagatacac agctggatgc cactggcaga gccaaacatc
     2881 gaggtttcgc cacgcccgta ggtgctggac ttggtcgccc agttgatttc attgatgccg
     2941 ctaatgtcgc ggtgagtggt tgcacggcaa tgcatgcgcg cgaccagatc agcgggaggc
     3001 gtccagccct gcaggtctaa tgcaaggtgg acggcacact ggttgatttc cacatgcgtc
     3061 atcacctggg acgcatagaa atccaagcga tcctgcaggc gctcaggcga caggctgtcg
     3121 attgcatggg gtgacacttc gattttcaga tgggggccga tgcagtccag cttggcgttg
     3181 aagttcttca ccagcaggat gatgcccagg tcagcattct gcagcttgta ctgatacccg
     3241 gaatcacgac ctacccggcc cgagtgccaa cgctggcccg cgaagtcgac cagggtgcct
     3301 ggcacctcaa acagcgccat gatctccggg cggatcagcc ctttgtacag ctggcgcacg
     3361 gtatcgacgc cgcaacggag caggcgaacg cctgacaggt cggtgaacgt gacgttgcca
     3421 gggtcaacaa acagacgccc gtgtttgtca tcttctgcag tcagagcatt caaacgctga
     3481 tgatctttta ccgttgccat tgctttacct attgctgagt aatgcggaag ttaatttctt
     3541 gttatctgac gtgttacagg gacgtcaccg ggaggcgctc gcgaggccgg cgagagcacc
     3601 ggctgcgccg aaacccacgc cggcctcgca gcgctgtctt cttcgtccgt ccataggcct
     3661 atctgtttat caacgggcgg tattggcgct actcggccgc tccagggggc taggaggatc
     3721 agcagcagga ttagcgcgag cagaggaggg cggaagtcgg gggcgcgcag tgggtggcgc
     3781 tggcgataca aggcaaagca caccaagggc gctgcccttg tcatcccgct cttgccgccg
     3841 agggctcggg agcatggggt ggagaagcta ccccatgctc ccgttgatag gctgttcata
     3901 ggcgtaccgc gtcaagggtg cgctgcgccc gtgcttccgt tcgacggaac ggtggagctg
     3961 ttccgacgag ccgggagcgc ggcccttgac acggccaaag aagagggtcg ggtcatacgg
     4021 tccagtacgt tgccggaact ctgccggtcg cggtgccgta tcgcccgaat ggtgcggata
     4081 tccgtgaaga acacttcatt aggatgtgcc tgcgtgttgc taacttcgat aaccaggaag
     4141 taaccatgct tgcagatgcc ttccatcggc agcacaacgc caacgatgtg catcaactgc
     4201 cagaacgaat ccggctcttg tcgccaacct tgttcaatca gcgcggtttt gccgaggtag
     4261 gtcagagcgg tggcgaggtc tactggcgag ttctgatcag acatggctgc tgcctccttc
     4321 catgaggtcg gcattgccct ggataaggtg aggcagaggt aaacggctta gtaccttgcc
     4381 tgatgtgcgc tggcctcggc tgcgagtagc cggaagagtg cgaatggtac ggatggagtc
     4441 gaagtaggtt tcatccggga aatcagtgct gccgatgaca gggacagtca ggaagtaccc
     4501 gatatcgtta acgccctgga ctggtacgac caccccaacg atatgcttga aaagccagat
     4561 aggctccggg tccccgtccc ataccagttc aacaagaacg ggctggccga tgtagctaat
     4621 agctgtagcg gcatccaggg agatacgttg gctaggcatg gcggtagtct cccgctttga
     4681 actgcttttt gcccttgagc agatccgcac gcaggcgggc cagattgacc atgcggaagt
     4741 cggccagccg catgcttggg acggtgccgt tatccaccca gtcgattgcc tcttctatag
     4801 atatgccgct catttcggcg aaggtcttca cgctgcacag gtccaggctg tcgtcactga
     4861 tcatgggttt tctcctgcag gggctgttcc ttggcgaagc tggcttccag gcggatgacc
     4921 atttcggcgt tcatcgagcg gcgggtagcg tgggcggctt gttggatctg caggcgcagt
     4981 gcggcgggca cgcgcagctt gaattgcggg tcagttcggc tcacagcgtc cactcctgtt
     5041 cgagaagctg ttgatgcaac agcgccacgt tgaccatggt gcgtttgccc agcttctgcg
     5101 aaggcaggta attacgctcc acccaagtac gtacaacgac gggttcgtcg cccatgccga
     5161 tccactcggc gaatttctgc cacggcatca agggcggtgc accgtgcagc ttttgggggt
     5221 caagaccttc aggtggtagc atcagcttgc tccgctatat tccgctatat cttgcattag
     5281 atgtcttaca cataatgcaa ggctcggaca aaatataagc caagacacca caaagaaacc
     5341 accactatat gcaagctttt tagatgcttt cggaatgata agagaaagat tgatatccat
     5401 atgggataag gaaaagcttt cggcccctaa gcttgagaag ctgacaggca tagaccggag
     5461 cagctggtat cacttgagaa atggaaagcg gcgtgccaac gaacaggaca ttgaggccat
     5521 cgtgcagatg ttccctcaat acgctttctg gattgcgagc gggcaagtag cgccggagat
     5581 tggccagaca agtcctgact acgatgaagc caacagaaac ttgaaccaac ccaacgcggg
     5641 atagcgctca cccaggaagt gactaggcgc tggtatgccc gagcacatgg actcaagtgc
     5701 gattagagca actaggaagg tgggggatat taaggtaagg aagactttta gcagtttctc
     5761 acgcacattt tgttagggtg agccctatga gattaataaa gactcaatca actcattgct
     5821 ttcctgtgct tttgataaaa gatcttggta gtaagattca actttgattt ttagctctaa
     5881 actttttgca acatgcacct tgaattcagc tagacactct tcaggtgtca tttcttcttc
     5941 tggttcgacg atctctgacg tgagtcccat agttctatag aaaagcccct tgctagtatc
     6001 cattatctct tgaagactgg aagctagggc tagctcagag gaggaaaatt ctttaggaag
     6061 aataggcatt cttaacgcca gagcctctga atattggtta atcgccgact cagctggttt
     6121 caataactta ggccagtgtt cgcgtttaat ctccgcatat attcgataat ctgaaaagct
     6181 agtaaagtcc tcttcctcgc tctgaaatat gaaatagtat gctgcctcat ctatataagc
     6241 atgattggta gaaatatatt ccaatagtgc ctttgcagca aaaacataaa atttattaag
     6301 ctccaaaata ctatcgatct ttctttctaa atatctagct ctttgcccgc tgagcacatg
     6361 aagatctgct ttgtagcttg ccacatcttg ctgaagagac ctatttaaag aattttttgt
     6421 ctcctccaat tcggattgaa gctcaacgtt tctttgagat agtttctcgg aatacttatg
     6481 atcaagatgt ttgaagaata tcttttcccc aaatttagca atcatggcaa tagttagtcc
     6541 tgctccggca gctaagccaa ctaaatactt tataatttca taaaattctt gaggcaagtg
     6601 gatctccttg cttgaaaaat agaattaact cataagcatc ccagatgcga aacctaaata
     6661 atacagagtg gaagtcacca tagatggcac gaactgtttg agcgaaaggg gacatattaa
     6721 ttattagcta cagcatctgc agaaaaatag ttcagccccc ttttcgattg ttatgcggca
     6781 tctaagccaa gaacgacacc agtatatggc aaggcccaag tattgcactt tatggtaatt
     6841 attgaacgcc ccctattaac ttgaacgtta tccatattaa tccctagaac ctcatacgtt
     6901 gaattcatat tctctggata attgattgta attgtaaagc gatctgtcag ttgcgacatg
     6961 gtccaagaaa gatatctttt aggatgagcc ttgtactttg cggaaataat tacatccaca
     7021 aattcattat caatgaattt ctctagtgaa gccctaaacc ctaggccgcc ctcaataagc
     7081 ttcatttcat tattattaag ttcataatag ccgtcatcat caactacaac atctccagaa
     7141 aattgtttgg atggctttat tttaacctga ccaaaatcaa aactaacata ctcttctcgc
     7201 gaagcgaccc aatttattga gtcttggatt ttccccgcaa cacttttagc tctaaagcgt
     7261 atgttttctt ctatttcaag gccgtcgcca ttcacagact taacatccat gaaataatta
     7321 atgtcttctc tgtatggaga gccgataaga tgctgtagtt cattttcaaa aaatgacaga
     7381 aaattgttct ggtttccctc taaatcaata tcgaaatatg ccctaaatgt gtctctttga
     7441 agctgcacaa gctcatcctt gtttaggctt ctgatgtatt ctttttgcct aattacttca
     7501 gatagccgtt gttggaagta cacttgccag tctttcaggc ccaagataac agatagaatc
     7561 cccatagtta ttagccctat agaaatggct agcaagaatt catagacaac accttgaaat
     7621 agatcgatag atacatcaat ggttttaaaa aatatcactg ctagggatat gatcacgcct
     7681 atgagtacaa cgaaagaccc tgaaacggtt attttattct tcatgaatat cgaatgtctc
     7741 cgttgcataa ttattgatta acgacatcac accttaagat gcttgataac gttccttgtc
     7801 atgttgtcaa accttgatat gcacagaaac aaagaaggaa aacgcgactc aggcaggcga
     7861 aaagcgacat gggatgcccc gtgcactaga acagccaagg tgcagtgatg gctagacgct
     7921 actgttaagc ctcaagactg tctagctggc gcgtcgaaaa agtatcgaga tcacagcgaa
     7981 gaaaagcgga tcaaagcagg tatgagtcag ctagagccaa ggaatagaag ggctttgcgg
     8041 gataacgcag gcagaaacag aaggttcgat ttcctcggcg aaaatgaggg cgtcccaaaa
     8101 gtgtcacagt cacagcaagg caaagccgac caatgcggga tagcacaatt cgtaaccatc
     8161 tgatttactt ggtttagcgg gctatagcgg tgtattgcga ggtgtcagga atgggttcaa
     8221 atccccccgg ctccaccaaa tcgaaacacc ttaaacccct gattttccta gcggaagtca
     8281 ggggtttttc tttgggtctc ccaaaaatga tcgaagccgc caaggtgaat tccgttacaa
     8341 caatctaaaa gcagagcagc gacatttgtc ggggccagcc agtgcgccgg ttgtacgtac
     8401 tggctacctt ccgtgagccc ctcagctaaa aactttgctg agcttctgcc ttcaaaaaat
     8461 ccacaaatgc ccgaagcggc gaaggtacat gccgacggtc gttgtagtac aggtaggggc
     8521 cgtcgaaggc ctgccaccag tcttccagta ccggtaatag cgtgccttgc ttgagcagcg
     8581 gcccgaggta ttcctcgaag gtgtagataa tccccacgcc gcctaccgcg gcgcccacct
     8641 ccaggtcctg ggatgagctg agcagcggcc cctggggcgg tacgcgaatg gtggcgccgt
     8701 ctttttcaaa cacccagacc cccagcttgc cgctctcgaa gcgatgaccg atcaggcgat
     8761 ggttaagcag gtcacgggga tgcagcggtg tgccgttctg ctgcagatag ccaggcgatg
     8821 cggcggcgac gaagcgctgg cggcgcgggc cgatgggcac ggcaatcatg tccttggcca
     8881 ggctttcttc gtagcgcacg cccgcgtcat agccggcggc gatgacatcg ataaaggtgt
     8941 tatccatcgt tacttcaatg cttaccccag ggcagcgttc gagaaacggg gggatcagct
     9001 taggcagcac gaagcgggcg acgggcaccg ggacgttaag gcgcagagtc ccaaccgggc
     9061 tcagtgcatt gtcatcgagg tcgccaaagg ctgtggctat ctcctcaagc gccgggcgta
     9121 accgctccag caggcgtgcg ccgacttccg tggggaatac gctgcgggtc gtgcgattga
     9181 gcagccggac gcccaggtcg ctttccagcc ggcgcacacc atcgctgatg gaagaagccg
     9241 acatgccgcg tttctgcgcc gccacccgga agccgccggc ctcgacgacc gcggcaaaca
     9301 gggcgacatc ggtcaggttc ggctggcgca tggctgctct ccaaaagcga aaggctggat
     9361 tgtacggttg ggcgtacata gcgtccattt caatgcggct tatcgccagc agtccacggg
     9421 tgaacaatgc ctcacgtcga cgcaccgcct tgcagcggct cgtcagctca ttccctacgt
     9481 gaggctcgaa caatgacaag cacaactcta cagctggaac ccatacgtac cacccgcgcc
     9541 agttggcgca gaggtctggc aacccttggc ctggccctga gctgcatgct ggaggtcggc
     9601 agcgtcagcg cccagagtca gcccaagccg gattggagcc tggcggacat accctcgcag
     9661 agtgggcgga ttttcctggt cacgggcggc accagcggca tgggctacga agacgccaag
     9721 gccctggcgg ccgctggcgc gcaggtggtg attgcggcac gcaacccaca gcgcggcgaa
     9781 gaagcgattg cccggatcaa gcaggaggtt gccgacgccc aggtgcagtt cgaagcggtg
     9841 gacctggcca atctggcctc ggttcaggcg ctgggtgaac gcctcaacgc gactttgccg
     9901 cgcctcgatg ggctgatcaa taacgctgcg atcatgtcgc cgccacaacg tggctcctcg
     9961 gccgatggct tcgagtcgca actggccacc aactacctgg gccacttcgc cttgaccgcc
    10021 cagctgctgc cgctactgcg taaaagcgac ggcgcacggg tggtgacgct gtccagcatc
    10081 gcggccgcgc gcggcgccat caacttcgac gacctgcagt ccgagcaggc ctacaacccg
    10141 ttcggcgcct atgcacagtc caaactggcc actctgatgt ttgccttcga gctgcaacgc
    10201 cgcagcgaag cgcaaggctg ggggattcag agcattgccg cgcaccctgg cgtggccgtc
    10261 accgagctga tcgagcgcgg cccaggcctg aatagcgagt ttggacgcaa ctgggccaag
    10321 gatcgcgagc gctaccactc cgccgcccag ggtgcgttgc cgacgctgta cgcagccacc
    10381 gcccccgagg cgcaacccgg cgcctattac ggcccgattg gtgaggagga aaagcgtggc
    10441 cctctggggc tggccagcgt accggcagcc gcgcaagacg ctgcagccgc cgctcgcctg
    10501 tggacggtta gcgaacagct gaccggtgtc agctaccgct gagcctcgcg ccaggagagt
    10561 catcatgctg tcagcaactc tggtgcagcg cctcggcgct gccattaacg ccgaacgtca
    10621 cgagttgaat cccgcgcttg cggggttcgc cctgtttttc tgcctgttct ccggctattt
    10681 catgttgcgc ccgattcgcg agtcgatggg catcatggcc ggtatcgaga acctgcaatg
    10741 gctgttcacc gccaccttcg tggtcatgct ggtggcggtg ccgttgttcg cctggctcag
    10801 ttcgcgggtg ccgcgcattc acttcgtcga ttgggtctac gggttcttct gcctgaacct
    10861 gttggccttc gccgggctgt ttcagctcag cgctgacaac gtctggctgg cgcgggtgtt
    10921 ctacgtgtgg atctcggtct acaacctgtt cgtggtctcg gtggcctgga gcctgatggc
    10981 cgatgtgttc gatgggcggc aggccaagcg gctgttcgcc tttatcgcgg ccggcgcgag
    11041 tgtcggcggt ctggtcgggc cggcgatgag tgcgctgctg gtcggggtta tcggccaggc
    11101 cgggctggtg ctgctggcag ccgtgctgct cggcgtcgcc ctggcactga aagcgccgct
    11161 gatgcgctgg cgggaaatcg gtggcgccgg cagaccgggt gcgcccatca cggagagcac
    11221 ccacaagccg gtaacgggta acccgttcag cggcctgacg cgggtcctgc aatcacctta
    11281 tttgtttgct atcgccgggt ttgtcgtgct gctcgctacc gtgaccacct ttctgtattt
    11341 cgaacaggcg cgcctggtgg ccgagcgttt tccagaccgc gagtcacaga tcagggtatt
    11401 cggcatcatc gatgtgatcg tgcaggccgg cgccttgctc tcgcagttgt ttatcaccgg
    11461 gcgcatcgcg cagaagctgg gcgtgcgcat gctgctggcg atcgtgccga tactggtttg
    11521 tgtgggcttt atcggcctcg ctctggcacc gaccttcgcc atgctggcgg cgctgatgat
    11581 cgtccggcgc atcggtgagt acgccttcgt caggccaggg cgggaaatgc tcttcgcgcc
    11641 gctggatgcc gagagtaaat acaaagccaa gggctttatc gatacggtcg tctatcgggc
    11701 cggtgatgcc atcagcggct ggctgaagag tggcctggac atgctcggcc agggcgcagt
    11761 actagtggcg ttggtcggtg cggcctgcgc cttgatctgg ggcgtgctgg gctggtacct
    11821 gggccggcag gcggacgagt tgagcaaatg cgcggcgcct gaggtgagtg agcgcgatgc
    11881 agcgctgctc gctggccaat agccctggcc tgctcaggct cgggcttggc tcactctgtc
    11941 cagtagatag accaggccgt ggtaatcgat gtcgccgtgt tggctcaggc cgatttcgca
    12001 ggtgcggctg gtgctgatgc cttcctcgca gtactgcacg gcggatttca ggctgcgcag
    12061 ggcatggcgg ttcagctcag gggtggtaaa gcccttgtcg ccggcaaagc cgcagcagtg
    12121 gataccttcg ggaatgatca cttctgaggt gcagcgacgg acgatatcga tcaacccctg
    12181 cgcctcgccc aggtgctggg tgctgcaggt gacatgcacc gccaccggtt tgtcctgtgg
    12241 ggtgatctgc aggcggtcga gcaactgttc gcggataaat ttcaccgggt cgtgcagttt
    12301 cagccgtgca tccagaccgt cctgaactag gcgcagggtg caagggctgg tgtcgcagta
    12361 gattggatcg aggccgccgc gactggcctt gagcagggcg tcgattagct cctgcttctt
    12421 gcgttcagcc tgctcggcat atcccttgga ggcaaagggc tggccgcagc agaggttgtc
    12481 cagatcagcc gggaacacca cctgaaagcc gcccttctcc agcagggcgc gggtcttgtc
    12541 gagcagcggc acttgttcgt tgtcactggc cgctgggccc atggcgcggg acacgcaggc
    12601 ggccagatag accacccgtg gccgcgcatc gtcattggtc ttgggcaggt gcaggcgttg
    12661 cagcggttgc ggcatggccg gggtccactg cggcagtttg ccctggctgg ccttgctgag
    12721 cgccgccgag gtgcggctaa gcagcggcgt gcccatcagc aggcgcgcgc cattggccac
    12781 atgcagcatc actcgcgcac cttgcatggc gcggcggaag ttattcgcca gccagtcggc
    12841 gctgcgctgg ctgttggcgt cgcgagcgcg cagctggcgc accaggtcgc cagtgttgat
    12901 acccaccggg cagcgctggg cgcacagacc ggtggcggcg caggtgtcga tgccctggta
    12961 ctggtaggcc gcttccagct cacgggtgtc gataccggcg cgctttttcg cctggatatc
    13021 gcgccagatc acgatgcgct ggcgcgggct cagggtcagt cccttggacg ggcagaccgg
    13081 ctcgcagaaa ccgcactcga tgcacttgtc gacgatcgcg tcggcggctg gcagcggctt
    13141 gagattcttc agatgaatct gcgggtcatc cgacagcacc acatctggat taagaatgcc
    13201 ttgggggtcg agcaggcgct tgatctgcca catcagctgg taggcgtctg tgccccactc
    13261 cagctcgaca aagggcgcca tattgcgccc ggtgccatgc tcggccttta gcgaaccgcc
    13321 aaactccact gccaccagct gcgccacgtc gtccataaag gccgagtagc gggcgatttg
    13381 ctcaggcgtt tcgaaggcct gggtaaagac gaagtgcagg ttgccttcca gggcatggcc
    13441 gaaaatgatc gcctcgtcgt agccgtgctt gtcgaacagc gcgagcaggc ggttgacgcc
    13501 ctcggctagc tgttcgatgg ggaaggtcac gtcctcgatg atcaccgtgg tgccggtctg
    13561 ccgtaccgca ccaacggccg ggaaggtgtc cttgcggatc ttccacagca ggttgtaaac
    13621 ggccgggtct tcgctgaaat ccacctgctt ttccaggggg aagtgggcga tagaggccat
    13681 gatcagttgc agctgttcat gcagcaggct ctggctggcg gcgcgggatt cgatcagcag
    13741 ggcgcaggcg ctgtcggaca ggcccttgac ccattccggc atgccgggct tgtcctgcac
    13801 cgagcgcagg ctgcggcgat cgagcagttc cactgcggac accggctgct gtttgagcag
    13861 ggttaccgcg cggcagcagc tctccacatc ggggaacacc agcagggcgc tggccttgtg
    13921 tgggtgatcg ggcacggtgt tgtaggttac cgcgctgata aagcccaggg tgccttcgga
    13981 gccgaccagc aggtggctaa gaatatccag cggttggtcg aaatccacca gggcgttcag
    14041 cgacagaccg gtggtgtttt tcagtcggta cttatggcgg atcttggccg ccagcgtgct
    14101 attggcgcgg gtctgccggc ccagctcggc gagcgcttcg agcaggtctg cgtgggtggc
    14161 gaaaaaacgc gtgacgctga gcgggtcttc ggtatccagc accgtgccat cggccagcac
    14221 cagacggatg ccggccaggg tgtggtagct gttctgtgcc gtgccgcagc acatgccgct
    14281 ggcgttgttg gcgactatgc cgccgatctt gcaggcgtta atcgacgccg gatcagggcc
    14341 gatcttgcgc tggaagggcg ccagcacggc atttgcctgg gcgccgatca ctccgggctg
    14401 caggcgaatc tggctgccct gcccacggac ttcccggccg ttccagttat cgcccagcac
    14461 tatcaatacc gagtcactga tggcctggcc ggacaggctg gtgccagcgg cgcgaaaggt
    14521 caccggtacc tgatgggcgc cggccagcct aagcagatcg accacttcca gctccgcctc
    14581 gacgcgcacc accagcttgg gaatcagccg gtagaagctg gcgtcggtac cgaaggccag
    14641 ggtcgacagc gggtcgtcga aacgccgctc ggcaggtatc aggcgttcga cggcggtgag
    14701 aaaagcggca ggcaggctca tgcaggctcc atagtccggt acggcgtcag gtgcagcgtc
    14761 aggacgcggc gtggtgtagt tcgcgcacca gcgaatcggc agtgatttcg ctgatcgatt
    14821 tagcacctgt cagcaccatg gccacgcgca tttccttctc aatcagatcc agcaggttgc
    14881 ttacccctgc gccaccgtcg gcggccaggg cgtagataaa ggcgcggccg agcatcaccg
    14941 tgtccgcgcc gagggcgatc atgcgcacca catccaggcc ggtgcggatg ccggaatcgg
    15001 cgagaatcgc cagctgacct ttcactgcat cggcaattgc cggcagggcg cgggcgctgg
    15061 acattacgcc atcgagctga cggccgccgt ggttggagac gacgatgccg tcggcgccga
    15121 agctcacggc gtccttggca tcctgcgggt cgaggatgcc cttgatgacc atcgggccgt
    15181 cccagaattc acgaatccat tccaagtcct tccagctgat cgacggatcg aaattgttgc
    15241 ccagccagcc gatatagtcg gccaggcccg tggggttgcc ccggtaggcg gagatattgc
    15301 ccaggtcgtg gggcttgccg agcaggccga catcccaggc ccagcgtggg tgggtcatgg
    15361 cttgcagcat gcgccgcagc ggcgcgttcg ggccgctcat gccggagtgg gcatcacggt
    15421 agcgcgcgcc tggcacgggc atatctacgg taaataccaa ggtggtgacg ccggcagcct
    15481 tggcgcgcgc cagggcgttt ttcataaagc cgcggtcttt cagcacatac agctgaaacc
    15541 acatcggtcg gtcgatggcc ggcgcgactt cttcaatcgg gcacaccgaa acggtcgaca
    15601 gggtaaaggg gatgcccttg gcggcggctg ccttggcagc ctgcacttca ccgcgacggg
    15661 cgtacatgcc ggtcagaccg accggggcca gggccaccgg catgctcagg gtctcaccca
    15721 acaggcgggt ttccaggctc agttccgaca tattgcgcag cacgcgctgg cgcagggcaa
    15781 tgtccgccag gtcggcaacg ttgcgcttga gcgtgtgctc ggcataggca ccgccgtcga
    15841 tgtagtggaa cagaaacggc ggcagccggc gttgggcggc ggcgcggtag tcggtggagg
    15901 cagaaataat catggagtca cccaggtgga gaaaaagggt ttgcagcgaa acgccaatgc
    15961 gcgccgtttt atcggcgcgc agtgtggcgc ttatcagccg gccattagcg ggtcgctaat
    16021 gcccagcacg taaacggcaa acagcgcgat caggccggta aacagcacgt aatacagggt
    16081 aggccagacg gtcttgcgca ggatgctgcc ttcgcggccc aacaggccaa ccgtggccga
    16141 ggctgcgacg acgttgtgga tcgccaccat attgccggcg gctgcgccaa tcgcctgcac
    16201 cgcgactacc agggcgctgg aaatgcccag gctgctggct acgccgaact ggaactggct
    16261 gaacatcatg ttgctgacgg tgttggagcc ggcaataaag gcgcccaggg caccgacgct
    16321 tggcgccagc agcgggtaga tgccacccac gctatcggcc acccagcgcg ccatcaggat
    16381 cggcatgctc gacagctcgg cggcgttaac cccggagttg atcaggatgc gcaccatcgg
    16441 cacggtaaac agcagcacaa agccggcgct tagcagcacg ctggacgact ccttcactgc
    16501 gctgcccagc tcacgcagtt tcatgccgtg gaagaagaag gtggcaatca ccacggccac
    16561 cagaatcccg cccggcaggt acagcggctg gaagttggca ccgatgccgg tttcgccgag
    16621 caggtcgggg aaattcagca tcactgcctt caatgcagtg ctgacctcgg ggaatacccg
    16681 gctgatcacc agcagcacgc cgaccagtac ataaggcagc caggcgcgca gggcgctcat
    16741 tggcttggca gtcagttggt cgagcttcat ctccacggtg cccagccact cggctggcca
    16801 tttgtccgcc ggggcgaaat cccaggtggt ttttggcagc agaaagccca tgcgcgcggc
    16861 gctggtcaca attgccagac caatcaagcc gccgcccagc gagggaaatt ccgggccgag
    16921 aaatacaccg gtcaaggcgt aagggatggt gaacgccagt ccggcgaaga tggcaaacgg
    16981 caacacttca aaaccggctt tccagctttt ctcggtgccg aagaaacgcg tcagcatcag
    17041 caccataacc agcggcatca cggtgccgac gatggcgtgg atgattgcca cttcactggt
    17101 gatcaactgc agaaactgcg cccaggacga accctgttca atcagttgcg cgccgatggt
    17161 ggccgagtcc aggccggtgt tgatgccgac gatgatcggt gtacccaccg cgccgaagga
    17221 taccggcgtg ctctgtacca gcatgccgag cattactgca gccatggccg ggaagccgat
    17281 ggccaccagc agtggtgctg caatggcggc gggtgtgccg aagccggacg cgccctcgat
    17341 aaagcagccg aacagccagg caatgatgat cgcctggatg cgccggtccg ggctgatggt
    17401 ggcaaaaccg gcgcgaatcg cggtgatacc gccggaatgc ttgagggtgt tgagcagcag
    17461 aatcgcgccg aagatgatcc acagcagacc gagagtgatc agcaatccct gcacggtgga
    17521 ggcgagtacg cggttaaagc tcatgtccca ggcaaacagc gcgacaccgg cggtaagcag
    17581 gtagaccagc ggcatggcgt gtttggccgg ccagcgcagg ccgatcaaca gaacggcagc
    17641 cagcaggatg ggggaaaaag ccagtagggc aagaaggcca gtggacatag cggtctctcc
    17701 ttaacgctgc gcgatgcgca tgggtgaatg cggatgcgca tcgggctgta gcgctgcggc
    17761 ggatataagc aatagagccg gcatagggag acctcgttgg attcttggaa ttgttttagt
    17821 ttggttaatt ggtaatacca atttacaaga ctgcgtcgtc agggtagaag tgtggcctct
    17881 agcctgtcaa ttagctgcct tacgactttg gtcgtaaagc tgcgccttgc taggcaaggg
    17941 aggcggcgat aggcttgccg gcagcgggcc gcaaaggccc atggcaggtg gtcaaaccag
    18001 tggagtgcgg gctatggaag tgggatcggt gcgtcagcgc cgtttgtcgg acgatatcgt
    18061 cagccagctg gagaccatga tcctcgaagg cacgctcaag gctggcgaac gcttgccggc
    18121 ggagcgggta ttatccgagc agttcggcgt ctcccggcca tcgctgcgtg aagcgatcca
    18181 gaagttggct gccaagggct tgctggtcag ccgccatggc ggcggcaact atgtggcgga
    18241 aaccctcggc tcgactttca gtgacccgct gctgcacctg ctggaaagca accccgaagc
    18301 ccagcgcgac ctgctggagt ttcgtcacac cttggaaggc tcctgcgctt tttatgccgc
    18361 gcaacgcgcc accgagctgg atcgccagcg cctgaccgac gcctttgccg tactgcagga
    18421 ttgctacagc cgcagcggca aggtgacccg cgccgaggag ggtgccgccg atgccaactt
    18481 ccacctggcg attgccgagg ccagccacaa cgccgtgctg ctgcacacca ttcgcgggct
    18541 gttcgacctg ctcaagcgca acgtggtgac caatatcggc ggcatgtacg cccagcgtga
    18601 tgaaacccgc gacatgctga tcgaacagca ccgcacgctg taccaagcaa ttattgaagg
    18661 ccgcgccgaa gacgcccgtg cgctgtccaa ccggcatatc gactacgtgc aggaagtgct
    18721 ggcagaagtg cagcaacagg cgcgccgcga agagcgggcc ctgcgccgag gcagtacgcg
    18781 ctagcggcct gctaaagtct gggcaggcga accgcgactt ccaggccacc gaggggcgag
    18841 tcgcccaggc tgatgctggc gcggtgcagc tctactaccc gtagcacgat ggataggccc
    18901 agccccgcac cctggccgtc gccaagccga tagaaacgct cgaagagttt ttcccgctgc
    18961 gcctcgtcta cgccggggcc actgtcctgc acgcgcagca tgatggcatc cacctgagcc
    19021 tccagctgca cttgaatgcg tcctccgtcg ggggtgtatt gcacggcatt gctgatcagg
    19081 ttctgcagca gggtgccgag gcttgggcca tcggctagca gccgatagtc tgctggttca
    19141 acggcgtcca ggttgagttc ctgatgacgc tccagggcca gcggaatcag ctccgccaac
    19201 tcgttgcgca caaacccgag caggtccagc tcgcccatgg ccagttgcac cacgttgggt
    19261 tccaggcgcg ccaaggtcag tagctgcgcc accactcggg tggcgcgttc gacgccgcta
    19321 ccgagctggc gcagggcatc gctgcgatcc tgcgggtcgg gcgcttccag ggcgttctgg
    19381 gcgtggatgc gcagcaccgc cagcggtgtg cgcagttcgt gggcggcgtc ggcaataaag
    19441 cgtttttcct gatccagcag ctggttgacc tgcatcagta ggcggttgag cgaggcgccc
    19501 atgggttcca gttcctgcgg cagtgggccg aaggtcagcg gcgcgaggtt atccggcgcg
    19561 cgcgccttga ttgattcggc catgcgctgc agtggtcgca aaccaaagcc cacggccagc
    19621 cagagcagca gggcgagcag tggcaggccg atcagcaacg gctgcagggt gcgccgggca
    19681 attttgccgg acagctcccc gcgcacgtct tcgcgctcac ccaccagcac ccagtgatgg
    19741 tcgacgttgt cgtgcagcac aaacacccgc cagcggtgct cgccgagggt cagagtgtgg
    19801 taaccgctga ggtatttggc caggcggttg aggcgttcac tcagcggttg ctcgccggcc
    19861 ggtaggttga ggccgagctg ggcaatcatc tccatcagca ggcccttggg ggcgctggct
    19921 gactggaata ccagctcgcc gttgtcatcg agcatctgga aggccagctt gctctcgtaa
    19981 tcgtgcccga gcaggccatc gaggttgcgc gggctgtttt tcagcagcag ggcttcgtcc
    20041 agcgccgcct gcagatcgcg gcgcgagtcg gcggacagct catggcgtac caggcccatg
    20101 agcatgcgcg aggtctgcgc cagctgggcg tcaaacagct cttccacctc atggctggcg
    20161 tcgaggtaaa tcttgtggct gatcagcccc agggtcacgc tgagcaagga caacagcagg
    20221 accagcaacc gcgcacggat cgagcgcaac accttcggca tgttatttat ccaccaggta
    20281 gccgacgccg cgcacggtac ggatcagctc ggcaaagaac ttcttgcgca ggtggtgcac
    20341 atgcacctcc agggcgttgc tttccggctc ttcatcccag ccatagagcg cctgctgcag
    20401 cttgtcgcgg gtcatcactc gacctggttg ggcgagcagt tcgtgcagca ggagaaactc
    20461 cttgcgcggc agattgaccg cttggccctg ataactgacg gactggctga ccggatcaag
    20521 gctgatgccg cggtattcca gcgccggttg cgggcggttg aaactgcgcc gtagcagggc
    20581 gcgcagacgt gccttgagtt cggcgacatc gaagggtttg acgaggtaat catcggcccc
    20641 ggcatccagc ccggcgatgc ggtcgctggt ggagtcgcgg gcggtcagca ccagcaccgg
    20701 caccggattg gcggcggcgc gcaggtgctt gagcacctgc aggccgtcca tgcgcggcag
    20761 ccctaggtcg aggatcgcca gctcgaaact ctcgctgctc agggcgtgca gcgcactgct
    20821 gccatcctgc acccagtcga cggtataacc ctcgggcttg agagcggtgc gaataccttc
    20881 gccaagggct ttgtcgtctt cgaccagcag aatgcgcatg cagatattcc cggttttttc
    20941 tattcaagct cgcgcaggcg agattagtcc agctcggctt tgaccttgct gagcaatgtt
    21001 gcgatttcct ctttgcgccc ggcatcggcg gacaggcgat tgggccgcgc tggcgcttgc
    21061 agggctttct ccagggcggc cttggcttcc tcgaagcgtt tggcgcgggc caggtagtcg
    21121 ccatagaagt agttggggtc gatgccatcc gggttgatcg ccagggcctg cttgagcatg
    21181 gtttcggctt tttcgtcatc accaaaaccc actggccagc cgggcacttg atagtacagg
    21241 ctgccgaggc tggtataagc cgagccggag agcgcctgcg ggtctagtgc caggccctgt
    21301 tccaggctgg ccttggcctg cttgaccaga ctgagggcac ccaggccgcc tttggccccg
    21361 gcataggtgc tgaggatgat gccgttccag atctgcagtt cggcagcgtt cggctcggcg
    21421 gcgacggctt tttgtgcctg ttcgctaagg cttgtgaagg ccttttcacg ttgttccttg
    21481 ggcagctggt aattgatctc tgcccagcgg tcttgcaggc tttgcagttg cgcctggccc
    21541 tgttcactga gggcgaagct gggcaggctg acgaggcaga gcagggcgca ggcggcgtta
    21601 cgaataaggt tcatgcgggt ttcctcttag gctttgctgc gggcaaagcg ctggataatc
    21661 ggcagttgtt tacgcagggc ctggtcaacc acacgcggca gcaggctgtt gaggcgcacg
    21721 aagaatcttt ccggccagcc gagatagcgc tcgtcgtctt cgcggcgaat ggcttgcagc
    21781 agttgttggg cgacttgctg tggctcgtcc atggcaacgt tcagctcatc attcatggcc
    21841 accacgttgg cggcgttcat gctggtacga gtagcgcgcg gggcgaagta gagaaccttg
    21901 accggggtgt cggccagctc gcggcgcagc gcctcggaga aaccgcgcag ggcgaacttg
    21961 ctggcgcagt aggcgctgaa gcccgggtaa ccgatggagc cgaaggtcga accgaggttg
    22021 accaccagtg cctcaccttg ctggcgcagc aggggtagca ggcgatgagt gagctgcagg
    22081 gtggcggtta cattgaggtc gatcagctcg gcgatggcgc tttcgtcatg ctgttccagc
    22141 aggccgaagt gattgacccc ggcggcgttg atcagggtgt tgaggccgcc gaagcgctgc
    22201 gcggcggcga gcaccgcctc acgaccactg cgttggcgaa tatcggcagc caccacggca
    22261 actttgccag ggtgcgcctg ggccagcgcc tgcaaggcct cggcttgccg gccaaccagc
    22321 agtagctgtg cgccttcggc cagcaaggct ttgagcagcg cctggccgat gccgccgctg
    22381 gcaccggtga tcacggcgcg gcattcagac agtttcattg cggctcctgg gcgctaagcg
    22441 gcaggctgcg gaatatatcg ccgtacaggc ggtagaccac cttggcggtg tgcaccacgg
    22501 cggccttgtc atcgtcgttg tcgagctgat tcatcaggcg cttgaaaaac tcgatatgtt
    22561 ccaggtccag gctgccgtgg gaactcaggt agctgaaggc tttgtttggc agttgcagct
    22621 tgtcctgaat caccccggcg acctgggtgg cgagggcaat gctggtgcct tccagcacat
    22681 tgaccatgcc gaagaaactc accgggttgt gccgggcgat gcggtcgtag acgtagctga
    22741 ccatcagctc ggtgggcagc ctgggcgtgc cgttgcgcac ggcctccttg tccgcgctgc
    22801 aggcggcgat gtcattgagt acccattcct ggtggccctt ctcctcgtcg atatattcgg
    22861 cgatggcctc gcgcagccac tccaggcgtt ccggcaggcg tgcgccacag gccatcagca
    22921 gcggcacggt gtgtttaacg tggtgatagg cctcggtgag aaaggccacg tagctgttca
    22981 ggctgacgct gccggccatg gcctgggcaa tgatcggcgc accgagcagg taattgcgct
    23041 cagcgttggt ttgttcgagc agggattcgt aaaagctcat caggcatctc cgtagcagga
    23101 ttcactggcc atcagttgcg cgatggcact ttggtaatgg ttgagcaggg cgttacggcg
    23161 caggcgtcca ttgctggtag ctagctcgtt ggcgctgctg aaaggctgtg cggcgcgcag
    23221 ccagtggtgc acgcgggcgt agtcgggcag tgattggttg gcggtagcca cggctttggc
    23281 cagttggctg tcgctggtgt tcgggaagcg cggtaccagc acggcaacgt tgcccggcag
    23341 ggcttcgcca tgcagccagg cctgggcgat gggcagttgc tgcaccagct cggcctcgac
    23401 ccattccggg ttgacgttgc ggccgtaggc ggtgatgaac tggtgtttct tacgcccgtg
    23461 cagaacaagg aatttgccgt cgaaatgccc caagtcgcca gtgcccagcc attctccggc
    23521 gggggagggt tcgcccagat agcccaatag gcgcggacct ttaacctcta cttcctgatc
    23581 cactcccagg cgtacctgca agtgtggcag gggctggccg acggtgccaa tacggcggtt
    23641 ttccggagta ttcaggcaca ccaccgaggc gcattccgac agaccgtagc cctcgaatac
    23701 tggcagcccg agggcgtcgg cgcgttgcag caattggctg gccacgcggc caccgccaac
    23761 ggcaataaaa cgcaggctgt tcggcactgg caggccgcgt tcggccgcgc taactagggc
    23821 cagcagcagc tgcggaagca gaatcaggct attgggctgc gcctgggcca gtgcgccgag
    23881 aaaacgtggc aggtcgaact gactggcccc cagcaggcca atctgcgcca tcggcatcag
    23941 ctcgatctgc gcgccggcca gcagcggggc ataaacccca gcgatattct ccagcagggt
    24001 ggccagctgc agtacgcaca ggtggcgctc gaccttacag ctggcactgg tttgcaccaa
    24061 actgtgggcc accgccagtt gcgtggcgat atccaggcac acgcccttgg gctggccggt
    24121 ggtgccggag gtgtaggtga tcttgcaggt gccggcatgc acggccgatg cctggggcat
    24181 ggggcgcaga tgcaggccgg gcagtgcctg gacgaagctg gcgtggggct ggacgctgat
    24241 caggcagtcg atgccggcgc tgtcgagtac atgggcctgc tggccgctgg aaaagaaccc
    24301 cggcacgggc acgcagatca gcccggcatg cagggtggct aggtcccaga gcacccactc
    24361 gataccgtta tccagggcca gggcgacacg ccgcgcacca agctgctgca gttgcgtact
    24421 gcgttgttcg atctcgctca acaactgggc gtagctcagt tgcctgtcgc cttcgcgcaa
    24481 ggcaagggcg tcgccaaggg cagccaggcg gtggaatagc ggtttagcgg caggccacat
    24541 ggggcatgtc ctccagggcg tagaaggctt ggtgggcgag gcgtgggtac gcacccagct
    24601 gcagcaggcg ctgatggccg gcgtagatat cgccggccat cacctggggc tggttgtcgt
    24661 agtaggtgcc ccaatccgct tgttcatcgc ccatgcagtc aaggctggcc gggccaaggg
    24721 gcagaggcgt caggcccagg cgctgaaagc tgttgagcaa ggccagggtg ccggtaaagc
    24781 acacccagcg ataacccagc gccaccagca gatcggtcag cgccacgatc agcaggcgcg
    24841 cggcaccggg gctgccggcg gccaagttgc cgacctccac cagttgctta cgctctggcg
    24901 cattggccac ctgctgcgca gtaatcacct gttcggccgg ttgcgccagg tagcgctcaa
    24961 gaaacagcgg gccactggcg gcgctgcgca aacccaccgc gccgagcagt tgcccggcac
    25021 tgtcttccag gctgagcagg cagggcatga aatggcggat acgcgcctga tgctgggcag
    25081 caaagcgttc gccaataaag gcttcaatcc ccgcgcggcg agggctggtg gcgttggcca
    25141 gatagaggct gagcggctgc tcgcggccga tctgtgcaag tgcagtgtcg tgatacaccc
    25201 aaggcagctc catatcagga acctcatctg agaacttggg tgcgagtttc tgtgttcagg
    25261 cttaacggca aattaagtca caaattaatt tgcgcggtgt gcgccacacg catttcattg
    25321 cttgcgctgc aaccgcaaca gcccatagcc tgcaaagcct tgccggctac ctgactgccg
    25381 gctggatttg ccctgctctt aaacgccaaa acgctcggag atagttatgc cccctatgga
    25441 tgctgagcaa caactggcga cccgtctggc cattctggaa aaccctgagg cgcaggactg
    25501 caccgtgtat cgcgctgatg atcaggaccc ggaagccgag gaagtggatc tgggtgatgc
    25561 aaaagtgctg ttctgcggcc cgttcgaggc ccctgccgac tgggatgcgg cggagcgtga
    25621 agattatttc ggcgacagcg cgcctgagct gttcgtcaat gcgcgtatcg agtgcgaagc
    25681 caaaccgggc tccaagggcc atttcaccgt ggacatcggc gactacgtag ccgcccagcc
    25741 tggtctgggt gaagttgtga tgttctacgt gcacgactac ctggaagacg acagcggctg
    25801 cacctatgtg ctgctgcgtg atgaggaagt gctggagtaa gagcctgctg ttgtagggtg
    25861 cgacgcgcac accaggagcc tgtagcctgt ctcggtgcgc acggcgcacc ctaccaccgt
    25921 ctatccgtca tctgccctgt agtaccaggt tattacccgc gtctgcctga tctgaacaac
    25981 actctgtgct tctcaaccca gaaagggagc aggcacatgg cagcgaagaa gattctgatg
    26041 ctggtcggcg actacgtcga agactacgaa gtgatggtgc cgtttcaggc actgctgatg
    26101 gtcggtcacc aggtgcatgc ggtgtgcccg gacaagaccg ccgggcagag cgtgcgcacc
    26161 gccatccacg attttgaagg cgagcagaca tacagcgaga agccggggca caacttcgcc
    26221 ctcaatttcg acttcgccca ggtcaaggcc gaggattacg acgcgctgtt gattcccggt
    26281 ggccgcgccc cggagtacct gcgcctgaat gcccaggtgc tggcgttggt gcaggccttc
    26341 gacaaggccg gaaaacccat cgctgcggtc tgccatggcg cgcagttact cgccgccgca
    26401 ggcgtgctaa agggtcgtga atgcagcgcc tacccggcct gtggcccaga agtgactctg
    26461 gcgggtgggc gctatatcga tatcgccgtg gaccaggccc atgtgcaggg caatctggtc
    26521 accgctccgg cctggccggc acaccccaac tggctggcgg cattcctcgg cctgctcggc
    26581 acgcagatca cgctataaac tattggggct ggccaggccc caacattcag ggaggtgagc
    26641 cgccatgtgc gagctgtatg tcaaagccga tccgattctc tacgaatccc gcgcccgttc
    26701 actgcgtatt cgtggcgtgg tcaccaccct gcgcctggaa aaccagttct gggacatcct
    26761 gcgtgaaatc gccgaagtcg atggcatgac caccaaccag ctgatcacca agctgtatga
    26821 cgaggtcatg gactatcgcg gtgaagtggt aaatttcgcc tcgttcctgc gcgtcagctg
    26881 cacccgctac cttagccagc gccgtgcacc ggctgagctc agcctggtgc gggcgatcca
    26941 atcctaacag gccgttgaaa aactacctgc gttggcaata ctgcgtttaa aacggcccgg
    27001 gacgcggcgt ctcggaatgc tcatgtacaa ctcgtacact ccgcttcctc ggcgctttga
    27061 ccagccttcg gctgttacta cgtagcgcct tgtcttgcct gcctcgccta cgtttttcaa
    27121 cggcctgtta cgcggtagct gagatacgca atgccgggtc acccagcggg tggcttagcg
    27181 catcaaagaa acgcagttcc acgttttgct ctgcgaacag gctggcgtag tgctgcttct
    27241 gctgcgtgat aaacagcggg ttgtgcgggt agatcgatat tgccagggtc ttcagttcgg
    27301 cttgctgcaa ggcctggatc aggtgctgat cctgcgccgc taggctatgg ccaaagatgc
    27361 acaggctgtc cttgggctgc gccagttggc tgtagcagaa cgacaggtag tcggagctgc
    27421 ggatggcctt gagcttgtcg gcgctgctgc cttcgttgac gaatagcgga atatcgccca
    27481 gctggttgat ggcgaagctg gccagcagag tgctttccga tgaattgagg atgcgcgtgc
    27541 tgccgtcgat attcttcacc agatgcatgc cgccgtgcag gtagagcatg cgtgtggcgt
    27601 tgccctggcg ctggcagtcg ccgggggcga aggttgaatc cgcgccgcgg aacatatctt
    27661 caaacgcctc tggggcctgc atcacggccc agtagttgag caggtcgtag ttacccgagt
    27721 agaccgtctg gtactggcta agcgccttgt tgatccgcgt caggctgtca gcctgcatct
    27781 ggctccaggg aatatgcacc gcgcgcacgg cgtggatcag cgcttccttg atggcaaagt
    27841 agcgatggcg cggcgacgag gaattgatgg tcagcgcggc attcacccgg atgctgttct
    27901 tcagcgcgct gagcacctgc tcgaagttac tggtatccag ggcgcggaac acgctgagtt
    27961 cggtcgcgct aagcgggcga ttgcgcgtgg tctgcgcctc gtcgaatagc gagaagtagg
    28021 cgaacttgcg ccatatcgcc aggctcgcgc cattgccgac cagcagctcg ggaaaaccga
    28081 tgctgctgtt cagggtgttc cagtcttcca gttcgccagt aaatgccggg gtttgcataa
    28141 tcgctagttg tcaccttggg ccgtgtcaga gtgcgacttt aacatgggcg cgacaactgc
    28201 cggcttggcc gctgcggccc attgcaccgc caggatgctg cccagcaccg caatcagacc
    28261 cagcagcgcc acgccggtga tgctctgccc gagcaatgcc cagcccagca gcaccgccac
    28321 cagcgggcta agcaacccca gggaagacac cgccaccggc gccagccgag caataccgcg
    28381 aaaccacaac acataagcta gcagcgcgcc gaacagcgac aggtaggcgt agcccagcca
    28441 ctgcaacggg cttaattgcg gcagcggtgg gtcgatcagc caggccaccg gcagcaggaa
    28501 cagaccgcca atcagcaact gccagccggt aaacgccagc agcggcacag tcggccgcca
    28561 gcgccgcgtc aggtaagtgc cgctggccat gcaggcggtg ccgacaaagg cggcggcaat
    28621 cccgatcggg tcccagctgg cccccggcgc tagcaacagg gcggccatcc ctgcgatacc
    28681 gaccacactt gcagcgaccg ccacagaggc cggccgctgc tgatccatcg accacaccag
    28741 acccatcacc agcagcggtt gaatggcccc aaccaccgct gccaagccgc ccggcaagcg
    28801 gtacgcagcg acaaacagca gcgcctggaa gaagccgata ttcagcgctg ccagcaccag
    28861 caagcgcggc cattcactgc gccggggcag gtagcggctg tacagcacca acaacaggcc
    28921 ggcgggcagg gcgcgcagca gcgcggcggt aaacggccga tctggcggca ggatttcggt
    28981 ggtgacgata taggtcgaac cccagatggc cggtgccatg gcggtgatca gtacatccag
    29041 ccaggtgcta cgcgatgctg agctgtccat gcatttgtct cgcattcaag ataaaatgcg
    29101 aggctaatag caacttgtct tgagttcaag ataaatttcc aggggtaagc caatggcagt
    29161 ccagcgacag atggatgcgg tcgatgcaat tcttcagcag tggcaccgct agcggcctga
    29221 tctggatgtc agccccatgg gcgtgatcgg ccggcttggg cgctgcgcat cgctgctgcg
    29281 gcgagaactg gacaaactgt tcagcaactt tggcctgacc tcctgggagt tcgacgtgct
    29341 ggccaccttg cgccgctccg gtgagcccta ccgcctggcc ccgactacgc tgttctcgac
    29401 cctgatggtc acctccggca ccatgactag gcagctgcag caacttgaag gctccggcct
    29461 ggtcagtcgt atagccaatc ccagcgatgc gcgcagcatg ctggtgcagt tgacgccagc
    29521 cggtctggaa ctgatcgacc gcgcggtaac cgcgcatgtg gccaatgaag cgcggttcct
    29581 ggcgccgatt gccgccaccg aactggcgca actggaagcg cggctatccg aactgctggc
    29641 ggtgctggag ccggctggcg agtgattttc tgcaaacccg gccgtgagca ggcctgttag
    29701 acgaggtgag tgatgagca
//
LOCUS       DASRUM010000149         5443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2241937, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000149 DASRUM010000000
VERSION     DASRUM010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2241937"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1440)
                     /gene="gpmI"
                     /locus_tag="VFF22_09875"
     CDS             complement(<1..1440)
                     /gene="gpmI"
                     /locus_tag="VFF22_09875"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HZX17155.1"
                     /translation="MSATPKPLVLIILDGFGHSDSPESNAIMAANTPVIDHLRATQPH
                     GLISGSGMDVGLPDGQMGNSEVGHMNLGAGRVVYQDFTRVTKAIRDGEFFDNAEITGA
                     VDKAVKAGKSVHILGLLSDGGVHSHQDHLAAMAELAAQRGAERIYLHAFLDGRDTPPK
                     SAQPSIELLDATFAKLGKGRIASLSGRYFAMDRDNRWDRVEQAYGLLVDGNGQFNAAT
                     AVEGLNAAYERGESDEFVKATTIGEPVRMEDGDAVVFMNFRADRARELTRAFVEPGFK
                     EFERKRVPQLADFVMLTQYAASIPTPSAYKPEALTNVLGEYMANNGKTQLRIAETEKY
                     AHVTFFFSGGREEPFAGEERILIPSPNVATYDLQPQMSAPEVTDKIVDAIENQRYDVI
                     IVNYANGDMVGHTGVFSAAVAAVECLDGCVGRIVAALDKVGGEALITADHGNVEQMAD
                     ESTGQAHTAHTCEPVPFIYVGKRPATIRAG"
     gene            1587..2000
                     /locus_tag="VFF22_09880"
     CDS             1587..2000
                     /locus_tag="VFF22_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZX17156.1"
                     /translation="MLANLIEFATTHYVLSGMFATLLALLIFTEARKGGQSLSSRELT
                     ALVNSEQGVVLDIRGQKDFSAGHIVGALNIPFEKLASRMVELDKHKAKTIIVVDAMGQ
                     HAGAVCRDLKKAGFTAAKLSGGVASWRGDNLPLVK"
     gene            2002..2256
                     /gene="grxC"
                     /locus_tag="VFF22_09885"
     CDS             2002..2256
                     /gene="grxC"
                     /locus_tag="VFF22_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HZX17157.1"
                     /translation="MQPVVIYSSDWCPYCIRAKQLLTHKGVAFNEIKVDGKPDVRAEM
                     TRKARQTSVPQIWIGDTHVGGCDDLYALERAGKLDALLKA"
     gene            2297..2782
                     /gene="secB"
                     /locus_tag="VFF22_09890"
     CDS             2297..2782
                     /gene="secB"
                     /locus_tag="VFF22_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HZX17158.1"
                     /translation="MTEQASNGAAQGEQNPQFSLQRIYVRDLSFEAPKSPEIFRQEWA
                     PSVAMDLNTRQKALDGDFHEVVLTLSVTVKTGEETAFIAEVQQAGIFLIKGLDAASMS
                     HTLGAFCPNILFPYARETLDSLVVRGSFPALMLAPVNFDALYAQELQRMQQAGEAATA
                     H"
     gene            complement(2822..3286)
                     /gene="trmL"
                     /locus_tag="VFF22_09895"
     CDS             complement(2822..3286)
                     /gene="trmL"
                     /locus_tag="VFF22_09895"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HZX17159.1"
                     /translation="MFHVILFQPEIPPNTGNIIRLCANSGCHLHLIEPLGFELDDKRL
                     RRAGLDYHEYATLKRHADLDSCLASIGQPRVFAFTTKGSQAFHQVSYQPGDAFLFGPE
                     SRGLPQEIRDSLPPEQRLRLPMREGCRSLNLSNTVAVAVYEAWRQQGFADAQ"
     gene            3285..3716
                     /locus_tag="VFF22_09900"
     CDS             3285..3716
                     /locus_tag="VFF22_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17160.1"
                     /translation="MRGSELEDEQHSTDPRPRPWVRVIASLAVFGFLIGLMIGRLLQP
                     DPLWLEQVDVREQGLELWFNVEPVPREEHAGGVFILRLQSFGRQQHGQLQVQGRLANW
                     RLQRERRDLLLRVVAARPLRGEWRAEEVDGRWRLVVSLVEQ"
     gene            complement(4006..5439)
                     /gene="ntrC"
                     /locus_tag="VFF22_09905"
     CDS             complement(4006..5439)
                     /gene="ntrC"
                     /locus_tag="VFF22_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HZX17161.1"
                     /translation="MSRSETVWIVDDDRSIRWVLEKALQQEGMTTQSFDSADGVLSRL
                     SRQQPDVIISDIRMPGASGLDLLARIRELYPRLPVIIMTAHSDLDSAVASYQGGAFEY
                     LPKPFDVDEAVSLVKRANMHAQEQQSLAAPVEQPRTPEIIGEAPAMQEVFRAIGRLSH
                     SNITVLINGESGTGKELVAHALHRHSPRAASPFIALNMAAIPKDLMESELFGHEKGAF
                     TGAANQRRGRFEQADGGTLFLDEIGDMPADTQTRLLRVLADGEFYRVGGHTPVKVDVR
                     IIAATHQNLETLVQAGKFREDLFHRLNVIRIHIPRLSDRREDIPTLARHFLSRAALEL
                     AVEPKLLKSETEDYLQQLPWPGNVRQLENTCRWITVMASGREVHVDDLPPELLSQPQE
                     SAPVSNWEQALRQWADQALGRGQSSLLDTAVPAFERIMIETALKHTAGRRRDAAVLLG
                     WGRNTLTRKIKELGMNIDSADDEDSDD"
BASE COUNT          944 a         1564 c         1821 g         1114 t
ORIGIN      
        1 gccagcgcga atggtcgctg ggcgcttacc gacatagata aatggcaccg gctcgcaggt
       61 gtgtgcggta tgcgcctggc cagtgctttc atcggccatc tgctcgacgt tgccgtggtc
      121 ggcggtgatc agcgcttcgc cgccgacctt gtccagcgcg gcgacgatgc ggccgacgca
      181 gccgtccagg cattccaccg ccgccacggc agcggagaac acaccggtgt ggccgaccat
      241 gtcgccgttg gcgtagttga cgatgatcac gtcatagcgc tggttttcga tggcatcgac
      301 gatcttgtcg gtgacttccg gcgcgctcat ctgcggctgc aggtcatagg tggcgacgtt
      361 cggcgaaggg atcaggatgc gttcttcacc ggcaaagggt tcttcacgcc cgccggagaa
      421 gaagaaggtg acgtgggcgt acttctcggt ttcggcgatg cgcagctggg ttttgccgtt
      481 attcgccatg tattcgccga gcacgttggt cagcgcttcc ggcttgtagg cgctgggagt
      541 cgggatgctc gcggcgtact gggtgagcat gacgaagtcg gccagttgcg gcacgcgctt
      601 gcgctcgaac tccttgaagc ctggttcgac aaaggcgcgg gtcagttcgc gggcacggtc
      661 ggcgcggaag ttcataaaca ccacggcgtc gccgtcttcc attcgtactg gttcgccaat
      721 ggtggtggct ttgacgaatt catcgctttc gccacgttcg tatgcggcat tcaggccttc
      781 cacggcagtg gcggcgttga actggccgtt accgtcgacc agcaacccgt aggcctgctc
      841 gacgcggtcc cagcggttgt cgcggtccat cgcgaagtag cggccgctga ggctggcgat
      901 acggcccttg ccgagtttgg cgaaggtggc gtcgagcagc tcaatggacg gctgcgcgct
      961 tttcggcggc gtgtcgcggc cgtcgaggaa tgcatgcagg tagatacgct cggcaccgcg
     1021 ctgggcggcc agttcggcca tggcggccag atggtcctga tggctgtgta cgccgccgtc
     1081 ggagagcagg ccgaggatat gcacggattt gccagccttg acggctttat ccaccgcgcc
     1141 ggtgatctcg gcgttatcga agaactcgcc atcgcggatg gccttggtca cccgggtgaa
     1201 gtcttgatac accacgcggc cggcgccgag gttcatatga ccgacctcgg aattacccat
     1261 ctggccgtcc ggcaggccga catccatgcc cgagccagaa atcaggccgt gtggctgggt
     1321 ggcgcgcaga tgatcaatga ctggcgtgtt ggccgccatg atggcgttgg attcagggct
     1381 gtcactgtgg ccgaagccat cgaggatgat cagtaccagg ggtttgggcg tggcgctcat
     1441 agaactggtt cgctctgggt cgatgggcgg aaagtaaggg caggcatttt acggccaagt
     1501 gcggcaaacg tcaccgtcgg gcggggtttg gtcgacctgt gcggctgtgt atactggccg
     1561 acattttacc gccccggaac cgcccaatgc ttgccaactt gattgaattc gccactaccc
     1621 actatgtcct gagcggaatg ttcgcgaccc tgctcgcgct gctgatcttc accgaagcgc
     1681 gcaagggcgg gcagagcctg agcagccgcg agctgactgc gctggttaac agcgagcaag
     1741 gcgtggtgct ggatattcgt gggcaaaagg acttttccgc cgggcatatc gtaggggcgc
     1801 tgaatattcc tttcgaaaaa ctcgccagcc gcatggttga gctggacaag cacaaggcca
     1861 agaccatcat cgtggtcgat gctatgggcc agcacgctgg cgctgtgtgc cgcgacctga
     1921 aaaaggcggg cttcactgcg gccaagctgt ctggcggtgt tgccagctgg cgcggtgaca
     1981 acctgccgct ggtgaagtga gatgcagcct gtcgtcatct actccagcga ctggtgcccg
     2041 tactgcatcc gggccaagca actactgacg cataaaggcg tggcctttaa cgagatcaag
     2101 gttgatggca agccagatgt gcgggcagaa atgactcgca aggcgcggca gacctcggtg
     2161 ccgcagatct ggatcggtga cacccatgtg ggtggttgtg atgaccttta cgccttggag
     2221 cgcgccggca agctcgacgc gctgctcaag gcttaattcc ctgtttaccc gtacctaacg
     2281 acaagaaggc tttgccatga cagaacaagc tagcaacggc gccgctcagg gcgaacaaaa
     2341 cccgcaattc tccctgcagc gcatctacgt acgtgacctg tccttcgaag cgccgaagag
     2401 cccggaaatc ttccgtcagg agtgggcccc tagcgttgcc atggacctca acacccgtca
     2461 gaaggccctg gatggcgact tccacgaagt ggtgctgacc ctctcggtga ccgtgaaaac
     2521 aggcgaagaa accgccttca tcgctgaagt gcagcaggcc ggcatcttcc tgatcaaggg
     2581 cctggacgcg gcttccatga gccacacctt gggcgcgttc tgcccgaaca tcctcttccc
     2641 ctatgcccgc gaaaccctgg acagtctggt cgtgcgcggc tcgttcccag cgttgatgct
     2701 ggctccggtg aacttcgacg ccctctacgc ccaggagctg cagcgtatgc agcaggctgg
     2761 cgaggctgca acggctcact aagctcgcgc tgctggataa aaacgcccta cggggcgttt
     2821 tttattgagc gtctgcaaaa ccctgctgac gccaggcttc atagactgcc acagcgacgg
     2881 tattggacag gttcaagctg cggcagcctt cgcgcatcgg caggcgcagg cgttgctcgg
     2941 gcggcaggct gtcgcgaatc tcctggggca ggccacggct ttccgggccg aacagaaagg
     3001 catcgccggg ctggtaactg acctggtgga atgcttgcga gcctttggtg gtaaaggcaa
     3061 acacgcgcgg ctggccgatg ctggccaggc agctgtcgag gtccgcgtga cgcttgagcg
     3121 tggcgtattc gtggtaatcc aggccggcgc ggcgcaggcg tttgtcatcc agctcgaagc
     3181 ccagcggctc gatcaggtgc aggtggcagc cgctgttggc gcagagcctg ataatgttgc
     3241 cggtattcgg tggaatttcc ggttgaaaaa ggattacgtg aaacatgcgc ggctccgagc
     3301 ttgaagacga gcagcattct acagacccgc gcccccgtcc gtgggtgcgg gttattgctt
     3361 cattggcggt gtttggtttt ttaatcggtt tgatgattgg ccggctgctg cagccggacc
     3421 cgctgtggct ggagcaggta gacgtgcgcg agcagggcct tgagctgtgg tttaacgtcg
     3481 agccggtacc gcgcgaggag cacgccggcg gcgtgtttat cctgcgtctg cagagctttg
     3541 gccgccagca gcacggtcag ctgcaagtgc agggaaggtt ggccaattgg cgtttgcagc
     3601 gtgagagacg ggatttgtta ctgagagtgg tggcagcccg tccattgcgc ggcgagtggc
     3661 gcgctgagga ggtggacggg cgctggcggc tggtggtcag cctggtggag caataaaaga
     3721 ggggaatccc cggcctgcct gtaccaaggt ccccaaaact gggattactt gagtctatgc
     3781 aggctgtgtg ccagttttat tacgtcggct gcgggccgcg taaatactgg gcttcagggt
     3841 ttccatctgc cgcgtagggt gctaggcaag ggctgggatg tgaaccgcag ctgtgcactt
     3901 gatcgatgtc ggtgcattgc tctggcgcat tttcagttgt ttgccggtgt aaatgcgaaa
     3961 cgggccctgc tgagcccgtt ttgcatatgg cgcaacgtga gcggtttagt cgtcgctatc
     4021 ctcgtcatcg gcgctgtcga tgttcatgcc cagttccttg atcttgcggg tcagggtgtt
     4081 gcggccccag cccagcagca cggcggcgtc gcggcggcgg ccggcggtgt gcttgagggc
     4141 ggtttcgatc atgatccgct cgaacgccgg cacggcggta tccagcaggc tggattgacc
     4201 acggcccaag gcctggtcgg cccattggcg cagggcctgc tcccagttgc tgaccggcgc
     4261 actttcctgc ggctggctaa gcagctccgg cggcaggtcg tcgacatgca cctcacggcc
     4321 cgaggccatc acggtgatcc agcggcaggt gttttccagc tggcgcacat tgcctggcca
     4381 cggcagctgc tgcagatagt cttcggtttc gcttttcagc agcttgggtt cgactgccag
     4441 ctcgagcgcg gctcggctga ggaagtgccg ggccagggtg gggatgtctt cgcggcgatc
     4501 cgacaggcgc gggatatgga tgcggatcac gttgaggcga tggaacaggt cttcgcggaa
     4561 tttgccggcc tgcaccaggg tttccaggtt ttggtgcgtg gcagcaatga tgcgcacgtc
     4621 taccttgacc ggcgtatggc cgccgacccg gtagaactcg ccatcggcca gtacgcgcaa
     4681 cagtcgggtc tgggtgtcgg ccggcatatc gccgatctcg tcgagaaaca gcgtgccgcc
     4741 atcggcctgc tcgaaacggc cgcgacgctg attggcggcg ccggtaaagg cgcctttctc
     4801 atggccgaac agttcggatt ccatcaggtc tttcggaatc gccgccatat tcagcgcgat
     4861 aaacggtgag gccgcgcgtg ggctgtggcg gtgcagggcg tgggcgacca gctctttgcc
     4921 agtaccggat tcgccgttga tcagcacggt gatattggag tgcgagagac ggccgatggc
     4981 gcggaacacc tcctgcatgg ccggcgcttc gccgatgatc tccggcgtgc gtggttgctc
     5041 gaccggtgcg gccaggctct gttgttcctg cgcgtgcata ttggcgcgct tgaccagcga
     5101 caccgcttcg tcgacatcga acggcttggg caggtactcg aacgcgccgc cctggtagga
     5161 cgccacggcg ctgtccaggt cggaatgggc ggtcatgatg atcaccggca ggcgcgggta
     5221 cagctcgcgg atgcgcgcca gcagatcgag gccactggcc cccggcatac ggatatcgga
     5281 aatgatcaca tcgggttgct ggcggctcag gcggctgagc acgccatcgg cgctgtcgaa
     5341 gctctgggtg gtcatgcctt cctgttgcag ggccttttcc aggacccagc ggatggagcg
     5401 gtcgtcatcg acaatccaga cggtttcact gcgactcatg acg
//
LOCUS       DASRUM010000150        10366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_240347, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000150 DASRUM010000000
VERSION     DASRUM010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 10366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10366
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240347"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..1306)
                     /gene="mtnA"
                     /locus_tag="VFF22_09910"
     CDS             complement(230..1306)
                     /gene="mtnA"
                     /locus_tag="VFF22_09910"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HZX17162.1"
                     /translation="MREQLLAAEKVRAIEWRDGALHLLDQRALPFEETWLSYHSAADV
                     AEAIRSMVVRGAPAIGITAAYAIVLAARARLQEGGDWRTPLQADFDLLAASRPTAVNL
                     FWALERMRDRLARLKSHDDPLALLEAEAIGIHQSDREANLTMAQLGVDLIRKHQGNQQ
                     NLLTHCNAGALATGGFGTALGVIRAAFHDGLLERVYADETRPWLQGSRLTAWELAADG
                     IPVTVNVDSAAAHLMKTRGITWVIVGADRIAANGDVANKIGTYQLAVNAMHHGVRFMV
                     VAPSSTIDMAMEHGDDIVLEERDASELLELGGKHIAGGIEALNPVFDVTPADLIDFIV
                     TEKGVVDRPDAEKMAQLMCRKRLH"
     gene            1456..2775
                     /locus_tag="VFF22_09915"
     CDS             1456..2775
                     /locus_tag="VFF22_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HZX17163.1"
                     /translation="MPQTPLDLLLLPTWLVPVEPAGVVLLEHGLGIRDGRIALIAPRA
                     EALKHSALQVLELPGRLLTPGLVNAHGHAAMTLFRGLADDLPLMTWLEKHIWPAEAKW
                     VNEQFVQDGTELAIAEQLKGGITCFSDMYFFPETASQLVHSSGIRAQITIPVLDFPIP
                     GARDADESLRKGLALFDDLKHHPRLSVAFGPHAPYTVSDDKLENIRMLAEELDAGIHM
                     HVHETAFEVQQSLEQRGERPLARLARLDLLGPRFQAVHMTQINDEDLALLVESNSSVI
                     HCPESNLKLACGFCPVEKLWQAGVNVAIGTDGAASNNDLDLLGETRTAALLAKAVAGS
                     ATALDAHRALRMATLNGARALGLDEQIGSLELGKAADMAVFNLSGLAQQPVYDPVSQL
                     IYAGGRACVEHLWVGGKQLLDGGQLTRLDEQRIIANARAWGAKIVQR"
     gene            complement(3049..5403)
                     /locus_tag="VFF22_09920"
     CDS             complement(3049..5403)
                     /locus_tag="VFF22_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZX17164.1"
                     /translation="MSKHQQQSASFLERLIFNNRPAVILLCLLISLFLFYQAAQVRPQ
                     TSFEKMIPLGHPYIQKMLEHRNDLANLGNTVRISVEAVDGDIFSKEYMETLRQINDEA
                     FYIPGVDRSGLKSLWSPSVRWTEVTEEGFAGGEVIPQTYDGSAESLEELRSNILKSGQ
                     IGRLVANNFKSSIIDLPLLETYADPNDQSKQIPLDYQKFSHELEEKIREKYQAQNPNV
                     KIHIIGFAKKVGDLIDGLMGVALFFAVAIAITFVLLLWFTHCFKSTLAVVLTTLIAVV
                     WQLGLLHTLGFGLDPYSMLVPFLVFAIGISHGVQKINGIAMASGEATDSLSAARMAFR
                     QLFIPGLVALLSDAVGFITLLLIDIGVIRELAIGASMGVAVIILTNLILLPVMISYVG
                     ISKKAVKISREEADKEHPFWRFISNCAHPMVAPISVVVAVAALAGGVWYGQNLKIGDL
                     DQGAPELHPDSRYNLDNDFVIRNYSTSSDVLVIMVKTAAEGCSTYPTLSAMDELMWKM
                     ENTEGVQSAISMVTVSRQVIKGMNEGNLKWETLSRNQDVLNNSIARADGLYNGDCSVA
                     PLLVFLTDHKAETLTRAVKVAEDFAKENNKDGLEFILAAGNAGIEAATNEVIAKSETT
                     MLLAVYAAGGIMCLLTFRSLAATLCVMLPLVLTSVLGNALMAFMGIGVKVATLPVIAL
                     GVGIGVDYGIYIYSRLETYLRQGLSLQEAYYETLKSTGKAVLFTGVCLAIGVATWIFS
                     AIKFQADMGLMLTFMFLWNMIGAIWLLPALARFLINPEKLRNKG"
     gene            complement(5419..6492)
                     /locus_tag="VFF22_09925"
     CDS             complement(5419..6492)
                     /locus_tag="VFF22_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HZX17165.1"
                     /translation="MRRTQAGRVVGSSRAPVLRVHSPIAKALSLCSVLSALMFASAPL
                     QAASDDTSHYSVTSAKAHQTLLLDVASLGKRLVTVGDRGHILLSDDNGASWVQAKVPT
                     KQMLTAVFFIDDTHGWAVGHDAQILASTDGGATWVKQFEDLEREAPLLDVWFKDRNNG
                     YAVGAYGALLETTDGGANWEDVSDRMDNEDAYHLNAIVEVKDSGLFIVGEQGAMFRSP
                     DWGQTWETLEGPYEGSLFGALGTDEAGALLVYGLRGHLFRSADFGTTWQAISLNTANN
                     GPLEFGLADGNRLADGSIVVVGHGGTVLKSTDSGRTFNVTNRADRLSLAGVAAMENGN
                     LILVGQGGVHAATPTGADLGQQQ"
     gene            complement(6703..8073)
                     /locus_tag="VFF22_09930"
     CDS             complement(6703..8073)
                     /locus_tag="VFF22_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HZX17166.1"
                     /translation="MLKKHTLIGAAIALALTAGSAFAAVAPAEAAKLGASLTPFGAEK
                     AGNAAGTIPEWTGGITQAPAAYKTPGQHHVDPFADDKPLFTITKANLDQYKDNLTPGQ
                     IAMFNTYPNSYQMPVYQTRRSGSAPQWVYDNTIKNASTAKLLDGGNGFSDAYGGIPFP
                     IPQNGVEALWNHIARYRGTYIVRRASEVAVQRNGSYSLVTSQQEAMFKFYNPKGTAAD
                     LNNIMFYYLSFTKSPARLAGGAVLVHETLDQVKEPRQAWGYNAGQRRVRRAPNLAYDT
                     PIAAADGLRTADDTDMINGSPDRYDWKLIGKKEIYIPYNSYKVTSPEVKYKDLLQVGH
                     LNPAFTRNELHRVWVVEGTLKSGSRHIYSKRTLFLDEDSWQAAVVDQYDGRGELWRVS
                     IAYLKNYYDLPTTWSALDSFHDLQARRYHVQNLDNEEPSTIDFSQAVPDDGYFKPAAL
                     RRRGTR"
     gene            complement(8140..10221)
                     /locus_tag="VFF22_09935"
     CDS             complement(8140..10221)
                     /locus_tag="VFF22_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HZX17167.1"
                     /translation="MEIKSRKPVLRFAPAKAGFAFVGILPLLVAAHAQAVEFSFADNE
                     ISGSIDTTVSYGQLWRVQGQDKTNNDINTNDGNRSFDTGLVSEVFKITTDMEVTYQNY
                     GAFVRGTAFYDTQIMDKRNDYYDTSLAYQPSQNVPNDDSFTRATRHSAGRNAEILDAY
                     VYGNWDVMDMPVTARVGRQVFNWGEGLFYRGGINTTNPVDAGKFRLPGAEVKEVLVPV
                     EAFSFNIGLTDNLSMEAFYQWNWKETAVDPVGTFFSETDLFADGGNTAYSEQTALAPV
                     RGLYQGLSGFGVGGLQGNDYFTSDGTIKVATVGSDLNAKNDGQFGVSFRYIAEELNST
                     EFGFYFVNYHAKEPTIYADINSAYAGLDLAAIAGAAGAQLDPLVRQGVVAGVQQNVNP
                     AFSAAALNNIISTGANGTTLGSAAVEGAILQQYQAALSGATNSAVGGMAAVDLAGQVN
                     GRREYAEDIRMYGVSFNTTVADASLFGELSYRPNLPIGIATTNDLLGDLLTQAVPLVS
                     GGVANIGGQMVTMNDQIHNYERVEAFNASLGTIYNFGPTMTFDSLFGVAELSSEHVRG
                     SDLQYESRNGTRYYAGRGNGSYISGYDRDDQINKNAYGYTLVASGTWNDVYAGVNLSP
                     FAVYKHDFEGNSHQTGNFIEGRKAYTLGLRASYLNSLEAEVQYTEFYGAGQNNGSRDR
                     DNIGLNVKYSF"
BASE COUNT         2383 a         3110 c         2757 g         2116 t
ORIGIN      
        1 ctttttctgg tgaaactccg cgccgctcgt cctgagtggt gtggagcctg tcgccgtgcc
       61 ttgaggctca tagcgactca cgacaaatca ataagttatg ccatgtatct gcgcaggtaa
      121 gaggcccata acgggcaccc cgaaaaccgc cggagcttac cacaatcacc gcggcgcacc
      181 tatccccagc cgcagccagc cgaccaggga tgcctacccg cagaaaaact caatgcagac
      241 gcttacggca catcaattgc gccatttttt ccgcatccgg acgatcaacc acgcccttct
      301 cggtgacgat aaagtcgatc aggtctgccg gggtcacatc gaataccgga ttaagcgctt
      361 cgatcccacc agcaatgtgc ttgccgccca gctccagcag ctcactggca tcacgctctt
      421 ccagcacaat gtcgtcaccg tgctccatgg ccatgtcgat ggttgaactg ggcgcgacca
      481 ccataaaacg cacaccgtgg tgcatggcat tcaccgccag ctgataggtg ccgatcttgt
      541 tcgccacatc gccattggca gcgatgcgat ccgcgccgac tatgacccag gtgatgccac
      601 gggttttcat caagtgcgcg gcagccgaat cgacattgac cgtcaccggg atgccgtcag
      661 ccgccagctc ccaggcggtc aggcgcgaac cctgcagcca gggccgagtt tcgtcggcgt
      721 atacccgctc aagcaaacca tcgtggaagg cggcgcgaat cacccccaac gccgtaccga
      781 agccgcctgt ggccagggcg ccggcattgc agtgggtcag caggttctgc tgattacctt
      841 ggtgcttgcg gatcagatcg acgcccagct gggccatggt caggttggct tcgcggtcac
      901 tttgatgaat gccgatggct tcagcctcca gcagcgccag agggtcgtca tggcttttca
      961 gccgtgcaag acgatcacgc atgcgctcaa gcgcccagaa cagattgacc gcggtcggcc
     1021 gcgaagccgc caacaggtcg aaatccgcct gcaacggcgt acgccagtca ccgccctcct
     1081 gcagacgagc acgcgcagcc agaacgattg cataagccgc agtgataccg atggccggcg
     1141 caccgcgcac caccatgctg cggatcgcct cggcgacatc cgccgccgag tggtagctca
     1201 gccaggtttc ctcgaaaggc aacgcgcgct gatcgagcag gtgcagagcg ccgtcacgcc
     1261 actcgatggc cctgaccttc tctgccgcta gcaattgctc gcgcatcaca cacctccgcc
     1321 ggaaaaagcc gaccattata cgcacaccgc cccgcccatc gcgccaacag cccgctacat
     1381 aaagcgtgtg cagcttcaag cctagggtgt tcctctataa actcgccact ttactgactg
     1441 ccggaagcac tacccatgcc gcaaaccccg ctcgacctgc tactgctgcc cacctggctg
     1501 gtccctgtcg aacctgctgg tgtagtgctg ctggaacacg gcctgggtat ccgcgacgga
     1561 cgtatcgccc tgatcgcacc ccgcgccgag gcgctcaagc acagcgcctt gcaggtgctt
     1621 gagttgcccg ggcgactgct cacgccaggc ctggtcaacg cccacgggca tgcggcaatg
     1681 accctgttcc gtggtctggc tgacgatctg ccactgatga cctggctgga aaagcacatc
     1741 tggccggccg aggccaaatg ggtcaacgag cagtttgtcc aggacggcac cgagctggcc
     1801 atcgcagaac agctcaaggg cggcatcacc tgcttctccg acatgtactt cttcccggaa
     1861 accgccagcc agctggtgca cagcagcggc attcgcgcgc aaatcactat tccggtactg
     1921 gatttcccga ttcccggcgc gcgtgacgca gacgagtcgt tgcgcaaggg cctggcgctg
     1981 ttcgacgacc tcaagcacca cccgcgcctg agtgtcgcct ttggccccca tgcgccctac
     2041 acggtaagcg acgacaagct ggaaaacatc cgcatgctcg ccgaggagct ggacgccggc
     2101 attcatatgc atgtgcacga aaccgctttt gaagtgcagc agagcctgga gcagcgcggc
     2161 gagcggccac tggcccgcct ggcgcgcctc gacctgcttg gcccgcgctt ccaggccgtg
     2221 cacatgaccc agattaatga tgaagacctg gccctcctgg tggaaagcaa cagcagcgtg
     2281 atccattgcc cggaatccaa cctcaagctg gcctgcggct tctgcccggt ggaaaagctc
     2341 tggcaggccg gggtcaacgt agccatcggc actgatggcg cagccagcaa caacgacctc
     2401 gacctgctcg gcgaaacacg taccgccgct ctactggcca aagccgtagc cggttccgcc
     2461 accgccctgg atgcacaccg cgcgctgcgc atggccaccc tcaacggcgc ccgcgcattg
     2521 ggcctggatg agcaaatcgg ctcgctggaa ctgggcaagg ctgcggatat ggcggtgttc
     2581 aacctctccg gcttggctca gcagccggtc tacgacccgg tatcgcaact aatctacgcc
     2641 ggcggccgcg cctgcgtaga acacctatgg gttggcggca agcaattgct cgacggcggc
     2701 caactgacgc gcttggacga acaacggatc atcgccaacg cccgcgcctg gggggcgaaa
     2761 attgtccagc gatagcatgg gcttgcgggg ctttagccgc gagcttttgc tattcaccac
     2821 gtagcccgaa caagcgcagc gcagtccgag gcatggatgc ttgcgcagcg tgctgtaagt
     2881 tgggcggtgg ctgcctcccg gagaggcctt ggccgcgaaa cttctagagc caaacacgat
     2941 tcgccggcag accacgccta caaaatccat ctgcaaaccc aacccgtaga caataaaaaa
     3001 ccgcgaccca agggccgcgg tttttagtgt tgcgactgcg tatcaggctt agcccttgtt
     3061 acgcagcttc tccgggttga tcaggaagcg cgccagagca ggcagcagcc agatcgcgcc
     3121 gatcatgttc cagaggaaca taaaggtcag catcaggccc atgtcggcct ggaacttgat
     3181 cgccgagaag atccaggtcg ctacaccgat cgccaggcag acgccggtga acagcactgc
     3241 tttaccggtc gacttaaggg tctcgtagta ggcctcctgc agcgacagcc cctgacgcag
     3301 gtaagtctcc agacggctgt agatataaat gccgtagtcg acaccgatac ccacacccaa
     3361 agcgattacc ggcagggtcg ccaccttcac accgatgccc ataaaggcca tcagggcgtt
     3421 gcccagtacc gaagtcagta ccagcggcag catcacacag agcgtcgcag ccaaagagcg
     3481 gaaggtcagc agacacatga tgccccctgc ggcatatacc gccagcagca tagtggtctc
     3541 ggacttggcg atcacttcgt tggtagccgc ttcgatacca gcgttaccgg ctgccaggat
     3601 gaattccagg ccgtccttgt tgttttcctt ggcaaagtct tcagcaacct taaccgcacg
     3661 cgtcagggtt tctgccttgt ggtcggtgag gaacaccagc agcggtgcca ccgagcaatc
     3721 accgttgtac aggccatcgg cccgagcaat ggagttgttc agcacgtcct gattgcgcga
     3781 cagagtttcc catttcaggt tgccctcgtt catgcccttg atcacctgac gcgacacggt
     3841 aaccatggaa atggccgact gcacgccttc ggtgttctcc atcttccaca tcaactcatc
     3901 catggccgac aacgtcgggt aagtcgagca accttcagcc gccgtcttga ccatgatcac
     3961 cagtacatcg gagctggtgg agtagttgcg gatgacgaaa tcgttatcca ggttgtagcg
     4021 cgaatccgga tgcagctcag gcgcgccctg atcgaggtca ccgatcttca ggttctgccc
     4081 gtaccacaca ccaccggcca gcgccgcgac cgcaaccacc acggaaatcg gggccaccat
     4141 cgggtgcgca cagttggaga taaagcgcca gaacggatgc tccttatcgg cttcttcgcg
     4201 gctgatcttc accgctttct tgctgatgcc aacataggaa atcatcaccg gcaacagaat
     4261 caggttggtc agaatgatca ccgccacacc catggaagcg ccgatggcca gctcgcggat
     4321 cacgccgata tcaatcagaa gcaaggtgat gaagcccacg gcatccgaca gcagcgccac
     4381 cagacccggg ataaacagct gacggaaagc catacgcgca gcggacagcg aatcggtcgc
     4441 ctcgcccgaa gccatggcaa tgccgttgat tttctgcacg ccgtgggaaa taccgatggc
     4501 aaagaccaga aacggcacca gcatcgaata cggatcgagc ccgaagccca gggtatgcag
     4561 cagacccagt tgccaaacca ctgcaatcaa agtagtcagc accaccgcca gagtgctctt
     4621 gaagcagtgg gtaaaccaca gcagcagaac gaaggtgatg gcgattgcca cggcaaagaa
     4681 cagcgccaca cccatcagac catcgatcag atcgccaact ttcttggcga aaccgatgat
     4741 gtggattttg acgttggggt tctgcgcctg gtacttctcg cggatcttct cttccagctc
     4801 gtgggagaac ttctggtaat ccagagggat ctgcttgctc tggtcgttcg ggtcggcata
     4861 agtctccagc aacggcaagt cgatgatgct cgacttgaag ttgttggcca ccagacggcc
     4921 aatctgcccg gacttgagga tgttgctgcg caactcttcg aggctttcgg cagaaccgtc
     4981 gtaggtttgc ggaatcacct cgccgccggc aaaaccttct tcagtcactt cggtccagcg
     5041 tacgcttggg ctccacagtg acttcaggcc agaacggtcg acacccggaa tatagaaggc
     5101 ttcgtcgttg atctgacgca gcgtctccat gtattccttg ctgaagatgt cgccatccac
     5161 cgcttccacc gagatacgca cggtgttgcc caggttagcc aggtcattgc ggtgttcgag
     5221 catcttctgg atgtacggat gccccagcgg aatcatcttc tcgaagctgg tctgcggacg
     5281 cacctgcgcc gcttggtaga acagaaacag actgatcaac aggcacagca gaatcacggc
     5341 cgggcgattg ttaaagatca accgctccag gaaggaggca gattgttgtt gatgcttact
     5401 catgcaaagg ctcccggctt attgttgttg gcccagatcg gcgccagtag gcgtagcagc
     5461 atggacgcca ccctgcccta ccagaatcag attgccgttt tccattgctg caacgcctgc
     5521 cagcgagagc cggtcggcac gattggtcac attgaaagtg cggccgctgt cggtgctctt
     5581 gagtacggta ccgccatgcc caaccaccac gatggaaccg tcagccagac gattaccgtc
     5641 agccagacca aactccaaag gaccgttgtt agccgtgttc aagctgattg cctgccaggt
     5701 cgtaccaaaa tctgccgaac ggaacagatg cccgcgcagg ccatagacca gcaacgcacc
     5761 ggcttcatca gtgcccaggg caccgaatag cgaaccctcg taagggcctt ccagggtttc
     5821 ccaggtctgc ccccagtccg gggaacggaa catcgcgcct tgctcgccca cgataaacag
     5881 gccggagtct ttgacttcaa caatggcgtt gaggtggtag gcgtcttcgt tgtccatacg
     5941 gtcgctgacg tcttcccaat tggcgccacc gtcagtggtt tccaacaagg caccgtaagc
     6001 gcctactgca taaccgttat tgcgatcctt gaaccagaca tccagcaacg gcgcttcacg
     6061 ctcaaggtct tcaaactgtt tgacccaagt ggcgccgcca tcagtgctgg cgagaatctg
     6121 cgcatcatgg ccgacagccc aaccgtgggt gtcatcaata aagaacacgg ccgtcagcat
     6181 ttgcttggtc ggcactttgg cctgaaccca gctcgcgcca ttgtcgtcgg acagcaaaat
     6241 atgcccgcga tcaccgacag tgaccaagcg cttacctaga ctggcaacat ccagcagcag
     6301 agtttgatgg gctttggcag aagtgaccga gtaatgactg gtgtcatcgc tggccgcttg
     6361 cagtggcgca ctggcgaaca tcagcgcgga tagcacacta cacagcgaca gcgctttggc
     6421 tatcggtgag tggacgcgga gcaccggtgc gcgggacgat cccacaacac gaccggcctg
     6481 ggtgcgccgc atgacgggct cactcatata ccttccccct tattcttata ggtagcacta
     6541 cacttttagc agtgcgccta tcctactgag ctttcaaaag gcctgacaat cggtgcgacg
     6601 ttatgttttg ttaaacccgc ccgtctacca tgccctgttc gactgataaa tagcaaaagg
     6661 ccgctacaac agtagcggcc ttttgctcaa ccgacacgcg tattaacgcg taccgcgacg
     6721 acgcagtgca gcaggcttaa agtagccgtc atccggcaca gcctggctga agtcgatggt
     6781 gcttggctct tcgttatcca ggttctgcac gtggtaacga cgggcttgca gatcgtggaa
     6841 agaatccagc gcagaccagg tggttggcag gtcgtagtag tttttcagat aggcaatcga
     6901 aacgcgccac agctcgccgc gaccgtcgta ctgatcaacc actgctgctt gccagctgtc
     6961 ctcgtcgagg aacagggtgc gcttggagta gatgtgacgc gaacccgact tcagagtgcc
     7021 ctcgaccacc cacacacggt gcagctcgtt acgagtaaag gctgggttca agtgaccgac
     7081 ttgcagcagg tccttgtact tcacctcagg gctggtgact ttgtagctgt tgtaaggaat
     7141 gtagatttcc ttcttgccga tcagcttcca gtcataacga tctggcgaac cgttgatcat
     7201 gtcggtgtcg tctgcagtac gcaggccgtc agcggcagcg atcggtgtgt cataggcaag
     7261 gttcggcgca cggcgcacgc gacgctgacc ggcgttatag ccccaggcct gacgcggctc
     7321 ttttacctga tccagggttt cgtgtaccag taccgcaccg ccagccagac gcgctgggct
     7381 cttggtgaag gacaggtagt agaacatgat gttgttcagg tcagctgcag tccctttcgg
     7441 gttgtagaac ttgaacatgg cttcctgctg cgaggtcacc agggagtagc tgccgttacg
     7501 ctgcacagct acttccgaag cgcgacgcac gatgtaagta ccacggtagc gtgcgatgtg
     7561 gttccacagc gcttctacac cattttgcgg gatcgggaac gggatgccgc cgtaggcgtc
     7621 ggagaagccg ttaccgccat caagcaactt ggccgtgctg gcgttcttga tggtgttgtc
     7681 atacacccac tgcggcgcag aaccggaacg gcgggtctgg tagaccggca tctggtagct
     7741 gttcgggtag gtattgaaca tcgcaatctg acctggggtc aggttgtctt tgtactgatc
     7801 caggttggct ttggtgatgg tgaacaacgg cttgtcatca gcaaacggat ctacgtggtg
     7861 ctggcctggc gtcttgtaag ccgccggtgc ctgagtaata ccaccagtcc actcagggat
     7921 agtgcccgcc gcgttaccgg ctttctcagc accgaacggg gtgagacttg cgcccaactt
     7981 ggccgcttcc gcaggtgcaa ctgcagcgaa cgcgctacca gcggtcagtg ccagcgcaat
     8041 ggcagcacca atcagcgtgt gctttttcag catcttgatt ctccgtgaaa taagtcagca
     8101 accgagtgcc cgcaggcact cgatattttc tagttatatt tagaaggaat acttgacgtt
     8161 cagaccgata ttgtcgcggt cacgactgcc attgttctgg ccagcaccat agaattcagt
     8221 gtactgaact tcagcttcta ggctattcaa gtagctggcc cgtagaccca acgtataagc
     8281 cttacgacct tcaataaagt taccggtctg gtgtgagttg ccttcgaagt catgcttgta
     8341 taccgcgaat ggcgagagat ttacgcctgc atatacatca ttccaagtac cagaggcaac
     8401 aagcgtgtag ccgtaagcat tcttgttgat ctgatcgtca cgatcataac cagagatata
     8461 ggatccatta ccacgaccag cataataacg agtgccatta cgcgactcat actgcaaatc
     8521 actgccacga acatgctcag aggacaactc agcaacacca aacaatgaat caaatgtcat
     8581 tgtaggacca aagttgtaaa tagttcccag cgacgcattg aatgcctcaa cgcgctcgta
     8641 gttatgtatc tgatcgttca tggtcaccat ctgaccaccg atattggcaa caccaccaga
     8701 aacaagcggt acagcttgag tcagcagatc acccaacaaa tcattcgtgg tcgcaatacc
     8761 aataggcagg ttgggacggt aggacagctc accgaacagt gaggcatctg caaccgtggt
     8821 attgaagctg acgccataca tcctgatatc ttcggcatac tcacgacggc catttacttg
     8881 accagccaga tccactgcag ccataccacc taccgcacta ttagtggccc cgctcaaagc
     8941 tgcctggtac tgctgtagaa tcgccccttc tacagcagca ctgcccaagg tagtaccatt
     9001 tgcaccagtg ctaataatgt tattcaacgc ggcagcacta aatgccgggt tcacattttg
     9061 ctgaacacca gccaccacac cttgacgtac aagcggatca agctgagcac cagctgcacc
     9121 agcaatagcc gccaaatcaa gcccggcgta agcactattt atatcagcat atatagttgg
     9181 ttctttggcg tggtaattaa caaaataaaa accaaactct gtcgaattca attcctcagc
     9241 aatatagcga aaagaaacgc cgaactgacc atcatttttt gcattaagat cactaccaac
     9301 agtggcaact tttattgtgc catcagaagt aaagtaatca ttaccttgca aaccaccaac
     9361 accgaaacca ctcaggccct ggtagagacc acgcacagga gccagagccg tttgttcact
     9421 gtaagcagta tttccgccat ctgcaaacag gtctgtttca gagaagaaag tgccaactgg
     9481 atcaacagca gtctctttcc agttccactg atagaaggct tccatggata ggttatcagt
     9541 caagccgata ttaaagctga aggcctcaac cggaaccaat acctctttaa cttcagcacc
     9601 cggcaaacgg aacttaccag cgtctactgg gttggtagta ttgatgccgc cacgatagaa
     9661 caaaccctcc ccccagttga atacctgacg gcctacacga gcggtaaccg gcatatccat
     9721 gacatcccag ttaccataga cataggcatc tagaatttct gcgttacgac cagccgagtg
     9781 acgagtagca cgggtaaagc tgtcatcgtt tggtacgttc tggcttggct gataagccag
     9841 gctggtatcg taataatcgt tgcgcttgtc catgatctga gtgtcataga acgcagtacc
     9901 acgaacaaaa gcaccgtagt tctgataggt cacttccata tcggtagtga tcttgaatac
     9961 ttcagatacc aaaccggtat caaaactacg attaccgtcg ttggtattga tatcgttgtt
    10021 ggtcttgtcc tgaccttgta cgcgccagag ctgaccgtag gacacggtgg tatcgatgga
    10081 gccgctgatt tcattgtctg cgaagctgaa ttccacagct tgggcatggg ccgccaccag
    10141 gagtggaaga atgccgacaa aagcaaaacc tgccttggca ggtgcgaaac gcaatacggg
    10201 cttacgggac ttaatttcca tcgaaacatt actccgtgga cagatcatct tttttgtacg
    10261 ccaaccgctg gggcacgtta tggccgtact gatgacgacc ttaaatgttg attggaaggc
    10321 aagcccccaa cgcgcttaca cgcacctcac tgtttggcgt gcacca
//
LOCUS       DASRUM010000151         3836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_911306, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000151 DASRUM010000000
VERSION     DASRUM010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911306"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..76)
                     /locus_tag="VFF22_09940"
     CDS             complement(<1..76)
                     /locus_tag="VFF22_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17168.1"
                     /translation="MSPAELHADSIVIDGLIIAKWNREL"
     gene            complement(962..2254)
                     /gene="gbcA"
                     /locus_tag="VFF22_09945"
     CDS             complement(962..2254)
                     /gene="gbcA"
                     /locus_tag="VFF22_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-betaine demethylase subunit GbcA"
                     /protein_id="HZX17169.1"
                     /translation="MDVTSTLSLGDPLEPARKATAEMLQTRERTFSLPQPFYNDERLF
                     KIDMQEIFHKEWLIAGMTCEIPAKGNFLTLQIGDNPIMVIRGADGVVHAFHNVCRHRG
                     SRLCTSDKGKVAKLVCPYHQWTYELDGRLLFAGTEMGADFDLKDYSLKPVNCKVAGGY
                     IFISLAENPPAIDEFLSTLAHYMEPYDMENTKVAVQSTLMEKANWKLVLENNRECYHC
                     NGSHPELLNTLLEWDDVTDPRATQAFKDQVSECTTRWDKDNIPYAHASFGLRNRIVRM
                     PLLKGTVSMTMDGKPGCKKLMGRITDPDLGSMRILHLPHSWNHCMGDHLIVFTVWPIS
                     AQETMVTTKWLVHKDAVEGVDYDVARLRQVWDATNDQDRRLAEENQRGINSTAYQPGP
                     YSKTYEFGVVNFIDWYSERLLNNLGAAPAPYLKGVAAE"
     gene            2605..3708
                     /gene="gbcB"
                     /locus_tag="VFF22_09950"
     CDS             2605..3708
                     /gene="gbcB"
                     /locus_tag="VFF22_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-betaine demethylase subunit GbcB"
                     /protein_id="HZX17170.1"
                     /translation="MTTNDFLNPVTTQTWSNGRHMVRCVKVIQETWDVRTFCFMADQP
                     VLYFFKPGQFVTLELEIDGQPVMRSYTISSSPSVPYSFSITIKRVPGGKVSNWLHDNL
                     QEGQELPVHGPVGLFNAMDFPADKVLYLSGGVGITPVMSMARWFYDTNANVDMVFVHS
                     ARSPKDIIYHRELEHMAARINNFSLHVICEKHGLGEAWAGYRGYLNQKMLELIAPDYL
                     EREIFCCGPTPYMNAVKRLLEANHYDMSRYHEESFGATPPEVRAEVKELAAEAADAPA
                     VPAADQHQVAFTSTGKSIRVDPGETVHAAAAKLGLHIPKACGMGICGTCKVLKTAGEV
                     SMEHNGGITDEDVAEGYILSCCSVPQGDVAIEY"
BASE COUNT          780 a         1081 c         1151 g          824 t
ORIGIN      
        1 acagttcgcg gttccacttg gcgatgatca gcccgtcaat aacgatgctg tcggcgtgta
       61 attcggctgg gctcatcagg cagtcccctt tttagcggtg tcgccaaacc cgttgttggc
      121 gctttggact gagcatatcc ccgaggacca aggcgaccgg gtgcaaaaac gaccggggta
      181 ttgccgaaag cgtcaaagct gagtcgccta tggataagcc gccaaatatg cggccagccg
      241 actgcattgc tgagaacgta gggagttagg acgaggggaa agggggtggc aggaatacag
      301 aaagcacaaa ggggagccta ggctcccctt ctaattggat gtgtgcttta tttttattat
      361 taggcgactg ttattgttgt tttgtcgctt tccatcgcgt catcgctttc ggtgattcca
      421 accggaatca aagtcaaacg tatttttttg gttgctgata cagccatcgc cattgcctaa
      481 acccctgcaa tgatccaacc ggcacaccga gctacctcaa ggtagtttta ttcttatcgg
      541 cctggttgcg ggaaaacccg aatcacatac cctttccaaa aaaatctgtt tgcgactcgt
      601 gggtaatctt gtttttgttg tgccttgagc cggtacgata tttctgctga tgcttgttat
      661 tgtttttgtg cgatagataa agcattctgc gtgccagttt ttaaaaaaac cttataaatc
      721 aaacatatac gaaaacgccc gcaattttac tgacgcaggc tgtcacaatt ctggggtggt
      781 actactcgat agtgttcccg ctgctggctg agcggtaaca cttgtgtgca gttagttgcg
      841 cacataggcg gggcggaagc gggactgaaa aaatacccgc gacaagccgc gggtagaaag
      901 gtggcctact agggcctcag tgtggagccg tcacggcggg cgaccaaccc gccagccctc
      961 gttactcggc tgcgacgccc tttaagtaag gcgcaggggc ggctcccagg ttgttgagca
     1021 agcgctcgct gtaccagtcg ataaagttga ccacgccaaa ctcgtaggtt ttcgagtagg
     1081 ggccgggctg gtaggcggtg gaattgatgc cgcgctggtt ttcttcagcc agacgacggt
     1141 cctggtcgtt ggtggcgtcc catacctgac gcagccgcgc cacgtcgtag tcgacgcctt
     1201 ccacggcatc tttgtgcacc agccacttgg tggtgaccat ggtttcctgg gcgctgattg
     1261 gccatacggt gaagacgatc aggtgatcgc ccatgcagtg gttccacgaa tgcggcagat
     1321 gcaggatgcg catcgagccc aggtccggat cggtgatgcg gcccatcagt ttcttgcaac
     1381 ccggcttacc gtccatggtc atcgatacgg tgcccttgag cagcggcatg cgcacgatac
     1441 ggttacgtaa accgaagctg gcgtgagcgt aggggatgtt gtccttgtcc caacgggtgg
     1501 tgcactcgct cacctgatcc ttgaacgcct gggtggcgcg cgggtcggtg acgtcatccc
     1561 actccagcag ggtattgagc aattccgggt gcgaaccgtt gcagtggtag cactcgcggt
     1621 tgttctccag caccagcttc cagttggctt tctccatcaa ggtggactgc accgccacct
     1681 tggtgttttc catgtcgtaa ggctccatat agtgagccag ggtgctgagg aactcatcaa
     1741 tggccggcgg gttttctgcc agggagataa agatatagcc gccggcgacc ttgcagttga
     1801 ccggtttcag gctgtagtcc ttgaggtcaa agtccgcgcc catttcagtg ccggcaaaca
     1861 gcaggcgacc gtccagctcg taggtccact ggtgataagg gcacaccagc ttggccacct
     1921 tgcctttgtc actggtacac agacgcgaac cccggtggcg acacacgtta tggaaagcat
     1981 gcaccacgcc atcggcgcca cgaatcacca tgatcgggtt gtcgccgatc tgcagggtga
     2041 ggaagttgcc cttggccggg atctcgcagg tcatgccggc gatcagccat tccttgtgga
     2101 agatctcctg catatcgatc ttgaacaggc gctcgtcgtt gtagaacggc tgaggcaacg
     2161 agaaggtgcg ctcgcgggtt tgcagcatct cggcggtggc cttgcgtgcg ggctccagcg
     2221 gatcgcccag gctcagggtg gaagtgacgt ccatcggtaa ctcctcaatg gctgtcgcct
     2281 gttgcgacgg ccgtaaaagt ggctaatcgg ttgctacgca aggtggcgtc attgccccgt
     2341 tctgcgcctg ccgatggcga ttggatgaaa gaggaattcg ctcgtgacct tttgcttcgg
     2401 ctggctgcga gtgtggcgct cggcgtgggc gaaaccttat ctatgggcga catggtcaca
     2461 tccgtttccg acgcggcaaa ccaaggcctg cgcggcaggt cgcgataagc atgtaaatgt
     2521 cgctggcaga taaatgagcg tgccggccag tacgcacaat ccgccccata caaggccgat
     2581 gtccggccgc gcggagagac gtccatgacg acgaacgact tcctcaaccc ggttaccacc
     2641 cagacctgga gcaacgggcg gcatatggtg cgctgcgtca aagttatcca ggaaacctgg
     2701 gacgtgcgca ccttctgctt tatggccgat cagccggtgc tgtacttctt caagcctggg
     2761 cagttcgtca ccctggagct ggagatcgac ggccagccgg tgatgcgctc ctacaccatt
     2821 tccagctcgc cgtcggtgcc ctacagcttt tccatcacca tcaagcgggt accgggcggc
     2881 aaggtctcca actggttgca cgacaacctg caggaaggcc aggagctgcc ggtgcatggc
     2941 ccggtcgggc tgttcaacgc catggacttt cccgccgaca aggtgctcta cctcagcggc
     3001 ggcgtcggca tcaccccggt gatgtcgatg gcgcgctggt tctatgacac caacgccaat
     3061 gtcgacatgg tgttcgtgca cagcgcgcgt tcgccgaagg acatcatcta ccaccgcgag
     3121 ctggaacata tggctgcgcg aatcaacaac ttcagcctgc acgtgatctg cgagaagcat
     3181 ggcctgggtg aggcctgggc cggttatcgc ggctacctca accagaagat gctggagctg
     3241 attgccccgg attatctgga gcgggaaatc ttctgctgcg gcccgacgcc ctatatgaac
     3301 gcggtcaagc gcctgctcga agccaaccac tacgacatga gccgctacca cgaagagtct
     3361 ttcggcgcga cgccgccgga agtgcgcgcc gaggtcaagg aactcgctgc cgaagccgcc
     3421 gacgcgccgg ccgtacccgc cgccgatcag catcaggtgg cattcaccag caccggcaag
     3481 agcattcgcg tcgacccagg cgagaccgta cacgccgcgg ccgccaagct tggcctgcat
     3541 atccccaagg cctgcggcat gggtatctgc ggcacctgta aggtgctgaa gaccgctggc
     3601 gaggtgagca tggagcacaa cggcgggatc accgatgaag acgtggccga gggttatatc
     3661 ctctcgtgct gcagcgtgcc gcagggtgat gtggcaatcg agtattgagg gcgttgtaag
     3721 cacaacaccg cacacagcaa aacgccgcac tcgccatcaa ggccggtgcg gcgtttttgt
     3781 ttgcgcggaa tcagctgcga tgggcctttg taggagcggg cttgcccgcg ataaga
//
LOCUS       DASRUM010000152         2303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_241501, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000152 DASRUM010000000
VERSION     DASRUM010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241501"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2059)
                     /gene="pbpC"
                     /locus_tag="VFF22_09955"
     CDS             complement(<1..2059)
                     /gene="pbpC"
                     /locus_tag="VFF22_09955"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452799.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HZX17171.1"
                     /translation="MTRVKPLYLLAIGIWHKRLWPLLRRPWLGLPLCLLLILLVADRL
                     FPLPLPGDDLARVVLAEDGAPLWRFADRDGVWRYPVSPGEVSPYYLEALLTYEDRWFY
                     QHPGVNPLALGRAAWQNLSGGRVLSGGSTLSMQVARLLDPHARSYSGKLKQLWRTLQL
                     EWHLSKDEILTLYLNRAPFGGTLQGVAAASWAYLGKSPSQLTRAEAALLAVLPQAPSR
                     LRPDRHPERAQAARDKVLNRLASFEIWPQSAINDALEEPVLLAPRQEPRLAPLLARRL
                     NTRSSPPLIRTTIDAALQRRLEDLLLGWRARLPERTSAAILVVEQPSMAVRAYLGSVD
                     IGDAKRFGHVDMINAVRSPGSTLKPFLYGMALDEGLIHSESLLQDVPRRYGDYRPGNF
                     AAGFSGAVSASEALATSLNLPAVQLLEAYGPKRFAGELRSAGVPLLLPPLAEPNLALI
                     LGGAGSRLEELVSGYSAFARGGMAARLRFQPQDGLHERRLLSPGSAWIVRRILSGQAR
                     PDRDPRAQLVQRPTLAWKTGTSYGFRDAWAIGVAPRYLIGIWIGRPDGTPVSGQFGLA
                     SAAPLLLQVHDLLVNRDSQRGIALPIDPQPAEVGVAAICWPLGQPLDSRDPNCRRQRF
                     AWTLDGTTPPTLPAQYQPLGLGLQESLWVNAAGLRVDASCPGATAQRLALWPAPLEPW
                     LPRR"
BASE COUNT          450 a          778 c          734 g          341 t
ORIGIN      
        1 cgcggcgtgg cagccagggt tccagcggcg ccggccacaa cgccagacgc tgcgccgtcg
       61 cgccggggca actggcatcc acgcgcaacc cggcggcatt gacccagagg ctttcctgca
      121 gccccaaacc cagcggctga tactgcgccg gcaaggtcgg cggcgtggtg ccatccagcg
      181 tccaggcaaa gcgctgacgc cggcagttgg gatcgcgact gtccagcggt tggcccaacg
      241 gccaacagat cgccgccacg ccgacttcgg cgggctgtgg gtcaatcggc agggcaatgc
      301 cgcgctggct atcgcggttg accagcagat catgcacctg cagcagcagt ggcgcggccg
      361 aggccaagcc gaactgcccc gacaccgggg tgccgtccgg gcggccgatc cagatgccga
      421 tcaaataacg tggcgctacg ccaatcgccc aggcatcgcg aaagccgtaa ctggtgccgg
      481 tcttccaggc cagggtcggg cgctgcacca gttgcgcgcg cggatcacgg tccgggcgcg
      541 cctggccact gagaatgcgc cgcacaatcc acgccgagcc aggcgacagc aggcgccgct
      601 catgcagccc atcctgcggc tgaaaccgca gacgcgcggc catgccgcca cgggcaaagg
      661 cgctgtaacc gctaaccagc tcctccagcc tactgccggc cccgccgaga atcagcgcca
      721 ggttgggctc ggccaatggc ggcagcagca gcggcacacc ggcactgcgc aactcgccgg
      781 caaaacgctt cggcccgtag gcttccagca gttgcacagc gggcagattc agcgaagtgg
      841 ccagcgcctc gctggcggat accgcaccac tgaaaccggc agcaaagttg cccgggcgat
      901 aatcgccata acgccgtggc acatcctgca gcagcgactc ggaatggatc agtccttcat
      961 ccagcgccat gccgtaaaga aaaggtttca gcgtcgagcc gggcgagcgc accgcgttga
     1021 tcatgtcgac atggccgaag cgcttggcat cgccgatatc taccgagccc aggtaggcgc
     1081 gcaccgccat gctcggctgc tcaaccacca gaatcgccgc cgaggtgcgc tccggcaacc
     1141 gcgccctcca acccagcagc aggtcttcca ggcgacgctg caaggcggca tcgatagtcg
     1201 tgcgaatcag cggcgggctg ctgcgcgtat tcagtctccg cgccagtaac ggcgccaggc
     1261 gcggctcctg acgtggggcg agcagcaccg gctcctccaa ggcatcgttg atcgccgact
     1321 gcggccatat ctcaaaactg gccaggcggt tgagcacttt gtcgcgcgcg gcctgagcgc
     1381 gctcgggatg acggtccggg cgcaaacggc tgggcgcttg cggcagcact gcgagtaacg
     1441 ccgcttcggc gcgggtcagc tggcttgggg atttgcccag ataagcccag ctggcggcgg
     1501 ccaccccctg caaggtgccg ccaaacggtg cgcgattgag atacagcgtg aggatttcat
     1561 ccttggacag atgccactcc agctgcagcg tgcgccacag ctgcttgagc ttgccgctgt
     1621 aactgcgcgc atgtggatcc agcagccgcg ccacctgcat cgacagcgtg ctgccacccg
     1681 agagcacgcg cccgccactg aggttctgcc aggccgcgcg gcccagcgcc agcgggttga
     1741 ccccaggatg ctgatagaac cagcggtcct cataggtcag caacgcttcc agataatagg
     1801 gcgacacctc accggggctg accggataac gccatacgcc atcacggtcg gcaaagcgcc
     1861 acagcggcgc accgtcttcg gccagcacca cgcgggccag gtcatcaccc ggcaaaggca
     1921 gcggaaacag ccggtcggcc actagcagaa tcagcagcag acacagcgga agccccagcc
     1981 agggacgacg cagcaacggc cataggcgct tgtgccagat gccgattgcc agcaggtaca
     2041 acggtttaac gcgggtcatg cgcggatcca ttccttgtat cgcgatctgt actgacaaac
     2101 ggccaacagc gctgccattc tgccaaatga ctggcacact ggcgcgcagt agcaatcaac
     2161 gcagggtcga gtggaacgag aactatgggt gtagaaggtt tttttgaatc ccttggcgag
     2221 gcagtcggct cggtgatccg ctttatcgtc gaaggcttga gcggcttttt cggcatgctc
     2281 ggcggcgcgg tcagcagctt tat
//
LOCUS       DASRUM010000153        31026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1423667, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000153 DASRUM010000000
VERSION     DASRUM010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 31026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31026
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423667"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VFF22_09960"
     CDS             complement(<1..1052)
                     /locus_tag="VFF22_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HZX17172.1"
                     /translation="MPKNGLLLALVLSLLSACDSSTPGPAEVTATQTTPVSSEAAKPA
                     VDRKALAERYAGRELTVVDVSEVQLEGASTLSISFSVPLDPEQKLAERLHLVDSKDGK
                     VDGAWELSDNLMEVRLRHLEPQRKLVLTVDAGLLGLNGKSLAAEQVTRLETRDLQASV
                     GFASRGSLLPTRLAEGLPVIALNVDKINVEFFRIKPESLPAFLNRWGRSSNLDNWEAR
                     SLLPMADLVYGGRFDLNPARNTRETLLLPIAGLEPFKQPGVYLAVMREAGSYNYAQPA
                     TLFTLSDIGLSARRYQNRIDVFTQALAGGDALGDVRLEILDAEGQVLAEGKTDGAGHG
                     ELPLPPKAEVLLAHQG"
     gene            1240..2583
                     /locus_tag="VFF22_09965"
     CDS             1240..2583
                     /locus_tag="VFF22_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZX17173.1"
                     /translation="MNAVRDAWQHTPTHKRVWALAAPMILCNLSVPLVALVDSAVIGH
                     LPHAHQLAAVAVGGSLYTLLTWAVGFLRMGTTGFSAQAAGRGDGGALRQVLLQGLLLG
                     VLLAVVLISLAIPLSSVALALMQPSAQLDELARSYLYIRLLGLPAALATYALVGWLLG
                     TQNARGPLAILLTTNLLNVALDLLFVLGLHWGVAGAAWASVIAEWSGALLGLYLARRA
                     LRAYAGTLDWAALRRWLNWRPLLAVNRDIFIRTLALQAVFFLITVQGTRLGDATVAAN
                     ALLLNGLLLAAHALDGLAHALEALCGHALGARDRSALRRTLIVAGGWSLFASLAFALF
                     FLLGGHWFISLQTNIDVVREAAFAYLPYLALLPLIAVWSYLLDGLFIGATRAREMRDA
                     MLQALLLSLPLAWALQPLGNQGLWLAFLSFMALRSLCLAGYAYRLTRADQWFAPR"
     gene            2597..3625
                     /locus_tag="VFF22_09970"
     CDS             2597..3625
                     /locus_tag="VFF22_09970"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HZX17174.1"
                     /translation="MLSPEMPCDEVLRQQILDDHELIDTPADPYLDTLVRVVREVFEV
                     KTVLVSLIDHDRQWFKSRIGLEITETPRTISFCAHAILGTQPLIVEDTYNDLRFHDNP
                     VVVNNPQIRFYAGQPLFSQEGQPLGTLCLIDPTPRQLTDKQVRLFIDMATLVEGYLKL
                     RHVSEQTAQLRAALSREQRKTMLDPLTQLWNRAGLNHFLPRQQEQADDLGLQLGVLFC
                     DLDYFKKVNDSHGHAAGDQVLWETARRISAAVRPQDVVTRSGGEEFVVLLQVHDEHEL
                     LQIAERIRNALHKEPITIDELQLNLTISIGAALRGPLEPATATMNRADQALYQAKGNG
                     RNRVELAS"
     gene            3654..4109
                     /locus_tag="VFF22_09975"
     CDS             3654..4109
                     /locus_tag="VFF22_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009877152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HZX17175.1"
                     /translation="MGQAIAAYLHYLSIFLLFALLSIEHVQFKLPLDLRRARSLIITD
                     IAYGICAGVVLFTGLARVLWYGKGLDYYLGNSLFHAKVGLFILVGLISVVPTFVFLNW
                     RNSLKAGEVPQVSPAKARLVIMVIRLELLLLLVIPLLAVLMARGYGVIG"
     gene            4276..4521
                     /locus_tag="VFF22_09980"
     CDS             4276..4521
                     /locus_tag="VFF22_09980"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HZX17176.1"
                     /translation="MAARKKAALDFEQSLTDLQNLVERLENGELSLEDSLTAFEQGVR
                     LTRDCQTALAQAEQKVQILMERDGELEEAPFDADQQA"
     gene            4518..5405
                     /locus_tag="VFF22_09985"
     CDS             4518..5405
                     /locus_tag="VFF22_09985"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HZX17177.1"
                     /translation="MIAAYQSSCQSRVDAALETLLSSPRPELERLYAAMRYSLFNGGK
                     RVRPLLAYAACEALGGEPERANAAACAVELIHAYSLVHDDLPAMDDDDLRRGQPTTHK
                     AFDEACAILAGDGLQSLAFEVLADATHNPHDADLRLAMLSALARAAGPAGMVGGQAID
                     LGSVGQTLDQNALEIMHRHKTGALIEASVQLGALASGQADDHALKALHSYARAIGLAF
                     QVQDDILDVESDTATLGKTQGKDQAHDKPTYPALLGLDAAKAYALELRDQALHALRPF
                     DQAAEPLRDLARYIVERRS"
     gene            5584..8871
                     /locus_tag="VFF22_09990"
     CDS             5584..8871
                     /locus_tag="VFF22_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012614346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZX17178.1"
                     /translation="MPVTTAPFAALLIANRGEIAIRIARAASELGIRSVAIYAEDDAA
                     SLHMRQADRAVALKGRGVPAYLDMDQLIEIAKAQGCDAVHPGYGFLAENAEFARRCQV
                     AGLCFVGPSAEVLQLFGDKAAARELAERCDVPLVAGINQAINLEQAHAFLAEHGAVML
                     KALAGGGGRGMRPVFGADKLDEAFARCQSEAQSAFGNNALYIEQLVSHARHIEIQVLG
                     DSTGVVSHLWERDCSLQRRQQKLVEIAPSPDLPPAIRERMIAAALKLAGAAQYQGLGT
                     FEFLLDAEQPERFYFMEANPRIQVEHTVTEQVTGVDLLHTQLKLAAGQSLADLGLQQP
                     PPLNGCAVQLRINLESMSSDGSTRPAAGTLTAYQPPSGPGLRVDGYGYAGYPVSPSYD
                     SLLAKLIAHAPDYPSALRRAYRALCEFRLEGVASNLHLLQNLLRLPQVTSYQLTTRFV
                     ENHLGELLAAQTQAHPHHFFAGTQTASQSTEQVQDAPPGCLPLLTHSTGVVVSLEVAE
                     GDAVAVGQNIAVLEAMKMEFVVSASHSGIVRQLAVQVGSALNEGQPLLFIEPAEVNTA
                     ASQSEQSLDLDHIRADLAEVLERHALTRDERRPQAVAKRRKTGQRTARENLADLLDDD
                     SFSEYGALAIAAQRRRRSLEELIEQSPADGLVAGIGTVNAEAFGSQAARCMAIAYDYT
                     VFAGTQGVMNHKKTDRMLGLAAQWRLPLVLFAEGGGGRPGDSDFVGVAGLDCHTFVGM
                     AKLSGLVPLVGVVSGRCFAGNAALLGCCDVIIATENATIGMAGPAMIEGGGLGSFTPE
                     QVGPVSVQGPNGVIDVLVADEAEAVRVAKQYLSYFQGTLSEWDCADQRELRHLIPENR
                     LRVYDIRQVISTLADTDSVLELRRQFAPGLITAFIRIEGKPFGLLANNPAHLGGAIDA
                     VAGDKAARFMQLCDAFDIPLLSLCDTPGFMVGPEAEKQATVRHVSRMFVAAASLSVPF
                     FTVVLRKGYGLGAQAMAAGSFHSPLFTIAWPSAEFGAMGLEGAVRLGFAKELAAIEEP
                     AARQQLFDKLVAKAYQNGKGINMASFLEIDAVIDPVETRSWLLRGLNATPKPATREGK
                     KRSVDTW"
     gene            8966..10864
                     /gene="dxs"
                     /locus_tag="VFF22_09995"
     CDS             8966..10864
                     /gene="dxs"
                     /locus_tag="VFF22_09995"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HZX17179.1"
                     /translation="MPTTFHEIPRERPLTPLLDRANTPDELRRLGEAELETLADELRQ
                     YLLYTVGQTGGHFGAGLGVIELTVALHYVFDTPDDRLVWDVGHQAYPHKILTGRREQM
                     GSLRQKDGIAAFPRRSESEYDTFGVGHSSTSISAALGMAIAAKLQNSKRKAVAVIGDG
                     ALTAGMAFEALNHASDVNANMLVILNDNDMSISKNVGGLSNYLAKILSSRTYASMREG
                     SKKILSRLPGAWEIARKTEEHAKGMLVPGTLFEELGWNYIGPIDGHDLPTLLATLRNM
                     RDLDGPQFLHVVTKKGKGFAPAEVDPIGYHAITKLEPINAPAAQPKQASGPKYSSVFG
                     QWLCDMAATDPRLVGITPAMKEGSDLVAFSERYPERYFDVAIAEQHAVTLAAGMACDG
                     AKPVVAIYSTFLQRAYDQLIHDVAVQHLDVLFAIDRAGLVGEDGPTHAGSFDISYLRC
                     IPGMLVMTPSDENELRRMLTTGHLFDGPAAVRYPRGSGPNATIEPGLKPLEIGKGIVR
                     RQGSNVALLVFGVQLSEALKVGEALDATVVDMRFVKPLDEALVRQLAASNELLVTIEE
                     NSVMGGAGSAVSEFLAYAGILKPVLHLGLPDYYVEHAKPAQMLAECGLDAAGIEAAIR
                     ARLASLSA"
     regulatory      10930..11145
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            11247..13157
                     /locus_tag="VFF22_10000"
     CDS             11247..13157
                     /locus_tag="VFF22_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZX17180.1"
                     /translation="MKLSRLALAVALAPSISFAASDTSSHELPPLVITRATPLKAPAP
                     ASVKVINRKQIEETAASSLLDVLRSQAGVQIRDTIGDGSRATFSLRGFGENAVNNTLI
                     LVDGRRLNQPSQAGADLNSVPLANIERIEIIRGAGTVLYGDQAVGGVINIVTRTPSSN
                     EGYLEASRGSHDLEAFRGHIYQQLGGGFSTYLNGESRTADNYRDHNNASYSNAFARLR
                     YDHESGHALYEYQTVDDELLLPGSIPLALRRADRKQSSSSDWSDSKTQVHRFAVEQRL
                     NQVWSGNFDYSQSDQDAVGSFFGSDFDQDTRSATLSPRITARFDNNLGRAEWLLGHDH
                     ITSDYQATWGTDFHQTLRDWYSQYSQNLGSDLTLTLGYRASEAEDRNKSAGKTHTDRE
                     DSSSIGLSWQANQQTRLFIKREDVLRWANVDDNGFVLPGVEFLEPQTGVSWESGIEWQ
                     DSVQRYQATLYRLDLKDELMYDSLIANPRPWDANAQGANINLDKTRRDGLLLEAERQL
                     SERLSIGGQYSYTDAEFRAGSFQGNKVPWVARNTASAHLSYLIVPGLRSYLEAVHTGS
                     RYYSGDDANSQHKAGGYTLLNAALSYDYQQFNSKLRLNNLTGKRYDAFASATSSYPAA
                     EEQVQLSVGYRF"
     gene            complement(13194..13991)
                     /locus_tag="VFF22_10005"
     CDS             complement(13194..13991)
                     /locus_tag="VFF22_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HZX17181.1"
                     /translation="MRAATLCLLLLLALPAAAVERVVTLAPSLTEIMLELDAGDLLVG
                     LLDGGERPPAVAQLPSVGRYGQLELESLLKLQPDLVLLWPDSISSAQRQQLQRMGIPL
                     LIVEPRKLADLAENFVEIGTRIGRAEQGRQLHQQFNNGLIALRQRYQREQPLRLFYQV
                     WDTPLYTIGGQQIISDALGICGAENIFAELTLPAPQVSVEAVLQANPEVILAGVQGQL
                     DAWQIWPSVAAVERKQLWLVPDAGLERPSFQMLGAVEKLCERLGQAR"
     gene            complement(14191..14607)
                     /locus_tag="VFF22_10010"
     CDS             complement(14191..14607)
                     /locus_tag="VFF22_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX17182.1"
                     /translation="MTRGEVRRRLALEWWRQLAVALVPLFVLNLLFGAEQKTGLLTMP
                     LFIAGLASMFVSLPLFSAYKRALVATEKALDTADEHDAWLELARVRRLAFLGAALPAW
                     IAAVAVFAGLEAIPLILLAISSIVLLYLYRIPRQLG"
     gene            complement(14604..15224)
                     /gene="ribA"
                     /locus_tag="VFF22_10015"
     CDS             complement(14604..15224)
                     /gene="ribA"
                     /locus_tag="VFF22_10015"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HZX17183.1"
                     /translation="MSVVFVAASQLPTPFGVFTMHGFLEQATGKEHVALTFGNVADGA
                     PVLGRLHSECLTGDALFSLRCDCGFQLEAALRAIANEGRGVLLYLRQEGRGIGLLNKI
                     RAYELQDGGADTVEANERLGFGADQRDYAICLPMLDHLGISQLKLMTNNPRKVKALGD
                     MGINVATRVPLQIDHNPHNKKYLATKAGKLGHMLGSLHQGEVEESL"
     gene            complement(15346..15993)
                     /locus_tag="VFF22_10020"
     CDS             complement(15346..15993)
                     /locus_tag="VFF22_10020"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003289139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HZX17184.1"
                     /translation="MQGIKCFILGQLLLAASCVWAAPQVRVVGLFPGAAVLNVDGQRK
                     LVKVGQVGPGGVVVVSADSRGAVLRVDGVERSYSLSREYSEGFAEAQKKQLSVAKGIG
                     GHYWVAGSINGQSIQFLVDTGATSVALNDEHARRLGIDYRVVGAPLQVNTASGTARGW
                     RVTLDRVKIGELEVLGVEAVVLEGGSPTEALLGMSFLSRVGWKVEQDVLVLESKY"
     gene            complement(16011..16739)
                     /locus_tag="VFF22_10025"
     CDS             complement(16011..16739)
                     /locus_tag="VFF22_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX17185.1"
                     /translation="MGRRCLILLFALLTCCTAQANAPMPEQVRIASEVWERHTHADGT
                     GLAWDIMRLVFEPAGVALQIQSVPYTRSIGLVQRGEADAWVGSYLNEVDDGVFYPSLP
                     YDADRIAALGLTDKPLPSLANIGEFRLVWIRGYEYQRYLPDVRQYREVQRRSGILGML
                     DLGHADFYIDALSEVDDLLATTRAQERYRSTILTALPLYLGFADNPRGRALAELYDRR
                     MELLLAEDRLRELFTRWQQPYPFD"
     gene            complement(16773..17303)
                     /locus_tag="VFF22_10030"
     CDS             complement(16773..17303)
                     /locus_tag="VFF22_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HZX17186.1"
                     /translation="MTDHPKQVPAEFVPPSVWRNPCHFLAFGFGSGTLPKAPGTWGSL
                     VALPFIPLLQMLPDWGYWLMLGITMLFGFWLCGKVADDLRVHDHEGIVWDEMVGMWIT
                     LWLVPDGWGWLLLGFLLFRLFDILKPWPIRWIDRHVHGGVGIMLDDVLAGVFAWMALQ
                     LCVWGWANGVAAALLG"
     gene            complement(17296..18264)
                     /gene="thiL"
                     /locus_tag="VFF22_10035"
     CDS             complement(17296..18264)
                     /gene="thiL"
                     /locus_tag="VFF22_10035"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016973954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HZX17187.1"
                     /translation="MGEFELIRHYFAAASCAQAGEGVALGIGDDCALLQVPAGEQLAI
                     STDTLVAGVHFPESSDPFLLGQRALGVSVSDLAAMAATPVGFTLALTLPSAEPDWLQA
                     FARGLDQMAQHCAIRLIGGDTTRGPLSLTLSVFGRVPVGMALTRKGAQVGDLLCVGGE
                     LGDAAGALPLVLGQRQAEPAVAEALLARYWSPIPQLDLGLALRGKATAALDISDGLLA
                     DCDHIAAASGVALLIEQAQVPMSAALLAFAGASAARQCALSGGDDYRLAFTLPAQYLA
                     DLQHAGWPVRVIGRVVAGQGVTLLDSQGQPVQPPRSGYQHFGSASD"
     gene            complement(18272..18763)
                     /gene="nusB"
                     /locus_tag="VFF22_10040"
     CDS             complement(18272..18763)
                     /gene="nusB"
                     /locus_tag="VFF22_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZX17188.1"
                     /translation="MSQTDGNGQQPQAPKKGPSSKTLARRQARTLAMQALYSWHMAGQ
                     QLNEIEAQFRVDNDFTAVDGAYFHEILHGVPRQKTEIDGAFESLLDRPLAEIDPVELS
                     ILRLSTYELKNRIDIPYRVVINEGIELAKVFGATDGHKFVNGVLDKLAPRLRADEVRA
                     NKR"
     gene            complement(18760..19236)
                     /gene="ribE"
                     /locus_tag="VFF22_10045"
     CDS             complement(18760..19236)
                     /gene="ribE"
                     /locus_tag="VFF22_10045"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HZX17189.1"
                     /translation="MTLKTIEGTFIAPKGRFALVVGRFNSFVVESLVSGAVDALVRHG
                     VSESDITIIRAPGAFEIPLVAQKVAQRGDFDAIIALGAVIRGGTPHFEYVAGECTKGL
                     AQVSMEFGVPVAFGVLTVDSIEQAIERSGTKAGNKGAEAALSALEMVSLLAQLEAK"
     gene            complement(19288..20367)
                     /gene="ribBA"
                     /locus_tag="VFF22_10050"
     CDS             complement(19288..20367)
                     /gene="ribBA"
                     /locus_tag="VFF22_10050"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HZX17190.1"
                     /translation="MALNSIEELVEDIRAGKMVILMDDEDRENEGDLIIASECVTAEH
                     INFMARFARGLICMPMTRERCETLKLPLMAPRNGSGFGTKFTVSIEAAEGVTTGISAA
                     DRARTVQAAAAKNAKADDIVSPGHIFPLMAQPGGVLARAGHTEAACDLARMGGFEPSG
                     VICEIMNDDGTMSRRPELEVFAAEHGIKIGTIADLIHYRLMHERTVERVSEQVLDTEL
                     GQFNLVTYRDGVEDTAHMALTLGTVCAEEPTLVRVHNMDPLRDLFLVNQPGRWSLRAA
                     MAEVAKDGSGVVLLLGNPLTGPELLALLDRQQPANPTTYSTVGAGSQILRDLGVRKMR
                     LMSSPMKFNAISGFDLEVVEYLPSK"
     gene            complement(20380..21042)
                     /locus_tag="VFF22_10055"
     CDS             complement(20380..21042)
                     /locus_tag="VFF22_10055"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HZX17191.1"
                     /translation="MFTGIIESIGSIRALTPKGGDVRVYVATGKLDLADVKLGDSIAV
                     NGVCLTAVELPGDGFWADVSRETLAHTAFISLKAGSKVNLEKALTPTSRLGGHLVSGH
                     VDGVGEVIARADNARAVQFTIRAPRELAKYIAHKGSITVDGTSLTVNAVNGAEFELTI
                     VPHTLAETIMVDYRPGSQVNLEVDLLARYLERLLLGDKAAEPKASGMTESFLAEHGYL
                     NK"
     regulatory      complement(21210..21368)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(21486..22601)
                     /gene="ribD"
                     /locus_tag="VFF22_10060"
     CDS             complement(21486..22601)
                     /gene="ribD"
                     /locus_tag="VFF22_10060"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HZX17192.1"
                     /translation="MNSDHAFMARALELARKGLYSTHPNPRVGCVIVRDGRIVGEGWH
                     ARAGEPHAEVHALRQAGDKARGATAYVTLEPCSHHGRTPPCADALINAGITRVVAAMQ
                     DPNPQVGGDGLLRLMHAGIAVQSGVLETEARALNAGFIKRMEKGLPFVRVKLAMSLDG
                     RTAMASGESQWITGPAARAAVQRLRARSSVVLTGADTVLADDARLTVRADELGLGAEL
                     SALAQARPPLRVLVDGRLRVPLSVPFFQAGAAMVATCAAASARDRYLDDGHELLAVPG
                     SNGHVDLRRLLVELANRGANEVLVEAGPRLAGAFARLGLVDEYQLFVAPKLLGSSARP
                     LLDLPLARMADAPALKIVEMRAVGDDWRIIAVPQPAV"
     gene            complement(22604..23068)
                     /gene="nrdR"
                     /locus_tag="VFF22_10065"
     CDS             complement(22604..23068)
                     /gene="nrdR"
                     /locus_tag="VFF22_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HZX17193.1"
                     /translation="MHCPFCGANDTKVIDSRLVAEGEQVRRRRECLACEERFTTFETA
                     ELVMPRLIKQDGSRQPFDEDKLRAGMQRALEKRPVSIERLEAAIAHIKHKLRATGERE
                     IKSLVLGELVMGELQKLDEVAYIRFASVYKRFQDLNEFREEIDRLSREPAKG"
     gene            23185..23640
                     /locus_tag="VFF22_10070"
     CDS             23185..23640
                     /locus_tag="VFF22_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HZX17194.1"
                     /translation="MKVPKPLQPLILVSLIALLTACASDSPPPAKPTTPAPIAAPAEA
                     APLPAYQRELSGSLLNVPNEADVELALLAVDERGRPQALLGNIQLRGTGNPLAFRLPF
                     NPETFNKHSRIELHGRANQAGRLILRLPEQPIARGETQALGELRLVPAP"
     gene            23637..24293
                     /locus_tag="VFF22_10075"
     CDS             23637..24293
                     /locus_tag="VFF22_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003304020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HZX17195.1"
                     /translation="MTAPAALQAALSELLGDAQLRAQTLPGTAIKLWLIDPANMDRCF
                     SPEETRLILEEPPYWCFCWASGLVLARWLAEHPEWVRGKRVLDFGAGSGVAAIAAAKA
                     GALEVVACDLDPLAIASCQANAALNDVQLSYSADFFAEADRFDLIIVADVLYDRANLP
                     LLDQFLSRGREALVADSRVRDFQHPLYQRLGILEACTWPDLAEPAEFRHVSLYHAERA
                     "
     gene            24380..25252
                     /gene="trxA"
                     /locus_tag="VFF22_10080"
     CDS             24380..25252
                     /gene="trxA"
                     /locus_tag="VFF22_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZX17196.1"
                     /translation="MSDSPYIFDIAGSASFEQLVIENSFQKPVLVDFWADWCAPCKAL
                     MPLLAKITEEYNGELLLAKVNCDIEQDIVARFGIRSLPTVVLFKNGQPVDGFAGAQPE
                     SAIREMLKPHVAEPAPAAADPMEAAQALFSEGRFADAEAVLKVLLTENNEHAAALILY
                     ARCLAERGELGEAEAVLGAVKGDEHKQALAGARAQLTFLRQAAELPDVASLKTRLAQN
                     ADDDEASYQLAIQQLARQHYEPALDALLKLFIRNRSYADGLPHKTLLQVFDLLGNDHP
                     LVTAYRRKLYQAIY"
     gene            complement(25454..25795)
                     /locus_tag="VFF22_10085"
     CDS             complement(25454..25795)
                     /locus_tag="VFF22_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17197.1"
                     /translation="MVRIRGQIGAWPVDLTVELDAQDWAQLAQHIPLEVAPAPAAAAA
                     PVANDALWQTTLELLRQAGQIEGPQLLGQLAGLAGGEAAGKRLLVRLRHCAQVRMETG
                     VDAPIYHWAGE"
     gene            25921..26496
                     /locus_tag="VFF22_10090"
     CDS             25921..26496
                     /locus_tag="VFF22_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2796 domain-containing protein"
                     /protein_id="HZX17198.1"
                     /translation="MRRLLFALPFALLPLAAIAAEHSHDHDHDHAEHSSLDAHEHGSA
                     RLNVVLDGQALELELESPAMNLVGFEHAAKSDADKTKVAAARSQLQDPQALFGLGAGD
                     CTLSETELESPLFEDDEHEHEEHHDKDSEHSEIHAHYHLDCKKPGELQQLNLAELFKR
                     FPATEKIQVQLIGPNGQQGLELTPAQPTLSF"
     gene            26642..27352
                     /locus_tag="VFF22_10095"
     CDS             26642..27352
                     /locus_tag="VFF22_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007984450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX17199.1"
                     /translation="MSSALIQLSNLGFAWPGQPELLDIDSFTLQSGESLFLKGPSGSG
                     KTTLLGLLGGVQKPNRGSIQLLDQDLSTLSASARDRFRVDHTGYIFQQFNLLPFLSVR
                     ENVELPCHFSKLRASRAMQRHGSVAQATSTLLAHLGLNEASLLERRAGDLSIGQQQRV
                     AAARALIGQPELVIADEPTSALDVDAREAFLELLFAECREAGSSLLFVSHDQSLARLF
                     DRSLSLAELNKTTRLAEI"
     gene            27355..28620
                     /locus_tag="VFF22_10100"
     CDS             27355..28620
                     /locus_tag="VFF22_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX17200.1"
                     /translation="MYLLRLALASLGNRRFTAWLTVFAIALSVCLLLAVERVRTEARA
                     SFANTISGTDLIVGARSGSVNLLLYSVFRIGNATNNIRWESFEQFAKHRQVKWAIPIS
                     LGDSHRGYRVMGTSPAYFEHYRYARTAQLQLSSGRAFADDPFEVVLGAEVAQALNYQL
                     NQELVLAHGVATISLVKHDDKPFKVVGILKRTGTPVDRTLHISLAGMEALHVDWQNGM
                     PARGAAKVSAEQARAMDLQPKQITAFLLGLNSKIATFSLQREINEFRGEPLLAILPGV
                     ALQELWSLMGTAEKALFVVSLFVVLTGLIGMLTAILTSLNERRREMAILRSVGARPWH
                     IASLLIVEAFALALAGVLLGLLLLYLGIAAAQGYVQANYGLYLPLNLPTRYEWTLLGA
                     ILSAALLMGCVPAWRAYRQSLADGLSIRL"
     gene            28631..29152
                     /locus_tag="VFF22_10105"
     CDS             28631..29152
                     /locus_tag="VFF22_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HZX17201.1"
                     /translation="MHRSLLATLLLTLALPLAAAEVRELTWSEMVPADAPPQVVEPAP
                     IHDLSQLADALAESGPAAMQQSPAAPVVQALDGQSVKLPGYIVPLDVTDEGRVTEFLL
                     VPYFGACVHVPPPPSNQIVHVTSELGVLLDALYQPFWIEGPLKVEQTSSELAEAGYQM
                     EAEKIYAYELPDS"
     gene            complement(29365..30462)
                     /locus_tag="VFF22_10110"
     CDS             complement(29365..30462)
                     /locus_tag="VFF22_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZX17202.1"
                     /translation="MYRLLLLIALCSAGAVQAAGQTVDPGVFRALDTAQTAQKKGDYA
                     AARRALDAAKGTPGSLEEALLWRSRGYLAWAEGQNAQAIELLEKVLKSGKLDAELQAA
                     EELNLARLNLVERRYAQVVTLLAPGQANANEEVLQMLVQAYQGLGQPAKALPLAERYV
                     QANPQAADSWLQFLVAMNADLNKYAAAERWQRQLLARQPNNAQGWRQLAALQQLSGSH
                     DKSLATLRAAYQKGLRFTESELDNLVLLAGAADQPWQGAKLLAGMLDQGLLPRTSARE
                     ERLGLLWWQARERSKSAQIFRELAQRSGSAKHWLHLAQLELEQARWQAGLDALAKAER
                     AGAERSKVRAWRQWAESELSYQREKHVASAG"
     gene            complement(30464..>31026)
                     /locus_tag="VFF22_10115"
     CDS             complement(30464..>31026)
                     /locus_tag="VFF22_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZX17203.1"
                     /translation="FVRLDGDSSDTATRTRQQAPQPPQAQTPQTPTPPTPMTATPSAN
                     LPALDLPVPSLSSGIAVNSAPTPSLTGLAAGASAPSAPAPSDAGGQPGGPESEVMPLN
                     DVSPDYPRYALQRGIEGHVKLAFTINRAGAVENVRVIEASPQNVFEREARRAAVRWRF
                     APRTEGGLAVAREAVKTLYFRLEGAR"
BASE COUNT         6090 a        10223 c         9212 g         5501 t
ORIGIN      
        1 tcgccctggt gggccagcag cacttcggcc ttgggcggca gcggcagttc gccatggccg
       61 gcgccatcgg tcttgccttc ggccagcacc tggccttctg catcaagaat ttccaggcgt
      121 acatcgccca gagcatcacc gccggccagg gcctgggtga agacatcgat acggttctga
      181 tagcgccgcg cggacaggcc gatatcactc agggtgaaca gcgtggccgg ttgcgcgtag
      241 ttgtaactgc cggcttcacg catcaccgcc aggtacacgc cgggctgctt gaacggctcc
      301 aggccggcaa tcggcagcag cagggtttcg cgggtattac gcgccggatt caggtcgaag
      361 cggccgccgt agaccagatc ggccatcggc agcaggctgc gggcttccca gttatccaga
      421 ttgctcgaac ggccccagcg gttaagaaag gccggcagag attcaggctt gatgcggaag
      481 aactcaacat tgatcttgtc gacgttcagc gcaatcaccg gcaggccttc ggcaaggcgt
      541 gtgggcagca aggaaccacg gctagcgaag ccgacactgg cttgcaggtc gcgggtttcc
      601 aggcgggtga cctgctcggc ggcgaggctc ttgccattca gcccaagcag accggcatcc
      661 acagtcagta ccagtttgcg ctgcggctcc agatggcgca ggcgcacctc catcaggttg
      721 tcgctcagct cccaggcgcc atcgaccttg ccgtctttgc tatcgaccag gtgcaggcgc
      781 tcggcgagtt tctgctcggg gtcgagcggc accgagaagc tgatcgacag ggtgctggcg
      841 ccttcgagct gcacttccga gacatccacc acggtcagtt cgcggccggc gtaacgctcg
      901 gccagcgcct tgcggtcgac cgccggtttg gcagcctcgc tgctgacagg agtggtttgg
      961 gtcgccgtca cttcggcagg cccaggggtg gatgaatcac aggcgctgag caggctcagg
     1021 acaagtgcca gaagcagtcc attcttcggc attgcgggct ccaacaaggg taggtgcaga
     1081 ggcctgacac tatagtcgag ccgtctatat acaactaagg ggcatgcaca aaattgctca
     1141 gcagcgcacg ccagtcgatc agtgtttaat aacccgcctg tgacacgcgc ccgcgcattt
     1201 cgttgcagcc tccgccaatc aagtttgaga gcgcatttga taaacgccgt acgcgatgcc
     1261 tggcagcaca cgcctaccca taaacgggtg tgggcgctgg ctgcgccgat gatcctctgc
     1321 aacctctcgg tgccgctggt ggcgctggtc gacagcgcgg tgatcggcca tctgccgcat
     1381 gcccaccaat tggccgcggt ggccgtgggc ggcagcctgt acaccttgct gacctgggcg
     1441 gtgggctttc tgcgtatggg caccaccggg ttcagcgctc aagcggcggg gcgtggtgat
     1501 ggcggcgcgc tacgtcaggt actgctgcag ggtttgctac tcggtgtgct gctggcggtg
     1561 gtgctgatca gtctggccat ccccctgagc agcgtcgcac tcgcgttgat gcaaccctcg
     1621 gcgcagctcg atgaactggc gcgcagttac ctgtatatcc gcctgctcgg attgcccgca
     1681 gcgctggcca cctatgccct ggttggctgg ctgctgggca cgcagaacgc gcgcgggccg
     1741 ctggccattc tgctgaccac taacctgctg aatgtcgccc tcgacctgtt gtttgtcctc
     1801 ggcttgcact ggggcgtggc cggcgcggcc tgggcctcgg tgattgccga atggagcggc
     1861 gccctgctcg gcctgtacct ggcccgccgc gccctgcgtg cctatgctgg gacgctggac
     1921 tgggcggcgc tgcgccgttg gttgaactgg cgaccgctgc tggcggtaaa ccgcgacatt
     1981 tttatccgca ctctggcgct gcaggcagtg ttctttctga tcaccgtgca gggcacccgt
     2041 ctgggcgatg ccaccgtggc ggccaatgcg ctgctgctca acggcctgct gctcgccgcc
     2101 cacgccctcg atggtttggc ccatgcgctg gaggcgctgt gcgggcatgc gttgggcgcc
     2161 cgagatcgca gtgcgctgcg gcgtacgctg attgtcgccg gcggctggtc gctattcgcc
     2221 agcctggcgt ttgcgctgtt ctttctgctc ggcgggcact ggtttatcag cctgcagacc
     2281 aacattgacg tggtgcgcga ggcggcgttc gcctacctgc cgtacctcgc cctgctgccg
     2341 ctgatcgcgg tgtggagcta cctgctcgac ggcctgttta tcggtgccac ccgtgcccgc
     2401 gaaatgcgcg atgccatgct ccaggcgctg ctgctaagcc tgccgctggc ctgggcgttg
     2461 caaccgctgg gcaaccaggg cttgtggctg gcctttctga gttttatggc cctgcgcagc
     2521 ctgtgcctgg ctggctatgc ttaccgcctg acccgtgccg atcaatggtt cgccccccgc
     2581 taatcaggag ccccacatgc tcagccctga aatgccctgc gatgaagtcc tgcgtcagca
     2641 gattctcgat gaccacgaat tgatcgacac gcccgccgac ccctacctgg ataccctggt
     2701 gcgggtggtg cgcgaagtct tcgaggtcaa gaccgtgctg gtcagcctga ttgaccatga
     2761 ccgccagtgg ttcaagtcac gcatcggcct ggagatcacc gaaaccccgc ggaccatctc
     2821 cttctgcgcc cacgcaattc tcggcaccca gccattgata gtcgaagaca cctacaacga
     2881 cctgcgtttt cacgacaatc cggtggtggt caacaacccg cagatccgct tctacgccgg
     2941 gcaaccgctg ttttcccagg aaggccagcc gctgggcacc ttgtgcctga tcgacccgac
     3001 accacggcag ctgacggaca agcaggtgcg cctgtttatc gatatggcaa ccctggtcga
     3061 aggctacctg aagctgcgcc acgtcagcga gcagaccgcg cagctgcgcg cggcgctgag
     3121 ccgtgaacag cgcaagacca tgctcgatcc actgacccag ctatggaacc gcgccggcct
     3181 taaccacttt ctgccccggc aacaggagca ggccgacgat ctgggactgc agctcggcgt
     3241 attgttctgc gatctggatt acttcaagaa ggtcaacgac agccatggcc acgccgcagg
     3301 cgatcaggtg ctgtgggaaa ccgcacggcg catcagcgcc gccgtacgtc cgcaggatgt
     3361 ggtgacacgc agcggcggcg aggagtttgt ggtgttgctg caggtgcacg atgagcatga
     3421 actgctgcag attgccgaac gcatccgcaa cgccctgcac aaggaaccca tcaccatcga
     3481 cgagctgcag ctcaacctga ccatcagcat cggtgcagcc ctgcgcggtc ctctggagcc
     3541 agcaaccgcc accatgaacc gcgccgacca ggcgctgtat caggccaaag gcaatggccg
     3601 taaccgggtc gagctagcca gctgatcaac caatccagag aagggacgcc accatgggcc
     3661 aagccattgc cgcctacctg cactacctgt cgatcttcct gctgttcgcc ctgctcagca
     3721 tcgagcatgt gcaattcaaa ctgccactgg acctgcgccg cgcacgcagc ctgatcatca
     3781 ccgacatcgc ctacggcatc tgcgccggcg tggtgctgtt taccggcctg gcgcgggtgc
     3841 tctggtacgg caagggcctg gactactacc tgggcaatag cctgtttcac gccaaagtcg
     3901 gcctgtttat cctggtcggg ctgatttccg tggtgccaac ctttgtcttc ctcaactggc
     3961 gcaacagcct caaggccggc gaagtaccgc aggtcagccc ggccaaggcg cgcctagtga
     4021 tcatggtgat tcgcctggaa ctgttgctgc tactggtcat cccattacta gcggtgctga
     4081 tggcgcgcgg ttatggggta atcggctgat aaccgagcag accaccaccc tgtgggaggc
     4141 gctttagcgg cgaaggctgt cgcggcttaa gcccctccca caagtgcaga taccgcccgc
     4201 tgatttgccc gtgcggcgct gctctgctag tgtcgcgccg gttccaattc cccgatctac
     4261 cccgagatat ccgccatggc cgcccgcaaa aaagccgcgc tcgacttcga gcaatccctg
     4321 accgatctgc agaacctggt cgaacgtctg gaaaatggcg agctgtcgct ggaagactcg
     4381 ctgactgcct tcgaacaagg cgtgcgcctg acccgcgatt gccagaccgc cctggcccag
     4441 gccgaacaga aagtacagat ccttatggag cgcgacggcg agctggagga agcccccttc
     4501 gacgcggacc agcaggcatg attgcggcct accagagcag ctgccaaagc cgcgtcgatg
     4561 ccgccctgga aaccctcctg agcagcccac gcccggaact ggaacgcctc tacgccgcca
     4621 tgcgctacag cctgttcaat ggcggcaaac gtgtgcgccc gctgctcgcc tacgccgcct
     4681 gcgaagccct cggcggtgag ccagagcgcg ccaatgcggc ggcctgcgcg gtagaactga
     4741 ttcacgccta ctccctggta catgacgatt tgccggcgat ggacgatgac gacctgcgcc
     4801 gcggccagcc gaccacccac aaagccttcg atgaagcctg cgcgatcctc gccggtgacg
     4861 gcctgcaaag cctagcgttt gaagtgctcg ccgacgccac acacaacccg catgacgccg
     4921 acctgcgcct agccatgctc agcgctctgg cgcgcgcggc tggcccggcc ggcatggtcg
     4981 gcggccaggc catcgacctc ggctcggtgg gccaaaccct cgaccagaac gccctggaaa
     5041 tcatgcatcg gcacaagacc ggcgcattga ttgaagccag cgtgcagctc ggcgcattgg
     5101 ccagcggcca ggccgacgac cacgcgctaa aagccctgca cagctacgcc cgcgccatcg
     5161 gcctggcctt tcaggtgcag gacgacatcc tcgacgtgga aagcgacacc gccaccttgg
     5221 gcaaaaccca gggcaaggat caggcccacg acaaacccac ctacccggcc cttctcggcc
     5281 tcgacgccgc caaggcttac gccctggagc tacgcgacca ggccctacac gccctgcgcc
     5341 cattcgacca ggcggccgag ccgctgcgtg atctggcgcg ttatatcgtc gaacgccgca
     5401 gctaagcgac gaatacctat cgcgggcatg gcccgctcct acacaaatcg cagcggtccg
     5461 taggagcggg ccatgcccgc gatcatcatc caaataaata tcccgaatta cgcccactag
     5521 gccttggttt acctcgcccg cagagcgcct aagatggctg cagaataaaa atctggagcc
     5581 accatgcccg ttacgaccgc gcccttcgcc gccctgctga tcgccaaccg tggcgaaatc
     5641 gccatccgta tcgcccgcgc cgccagtgaa ctgggcatcc gctcggtggc catctacgcc
     5701 gaagacgatg ccgcttccct gcatatgcgt caggccgatc gcgcggtagc gctcaagggc
     5761 cgaggtgtgc cggcctatct ggatatggat cagctaatcg agattgccaa ggcacagggc
     5821 tgtgatgccg tacaccccgg ctacggcttt ctcgcggaaa acgccgaatt cgcccgccgc
     5881 tgccaggtcg ctggcctgtg ctttgtcggc cccagcgccg aggtgctgca gctgtttggc
     5941 gacaaggccg cagcgcgaga gctggccgag cgctgcgacg tgccgttggt ggcaggcatc
     6001 aatcaggcga tcaacctgga gcaagcccac gcctttctcg ccgagcatgg cgcggtgatg
     6061 ctcaaggccc tggccggcgg cggaggccgt ggcatgcgcc cggtgttcgg tgccgacaag
     6121 ctggacgagg cctttgcccg ttgccaatcg gaggcgcaaa gcgcctttgg caataacgcg
     6181 ctgtatatcg agcaactggt cagccacgcc cggcatatcg aaatccaggt gctgggcgac
     6241 agcacgggcg tagtcagcca tctatgggag cgcgactgca gcctgcagcg ccgccagcag
     6301 aaactggtgg aaatcgctcc cagtccggat ctgccgcccg ccatccgcga acgcatgatc
     6361 gccgccgcgc tcaagctagc cggtgcagcg cagtatcagg gcctgggcac cttcgaattt
     6421 ctgctggatg cagagcagcc cgagcgcttc tactttatgg aagccaaccc gcgtatccag
     6481 gtcgagcata cggtcaccga acaggtcacc ggcgtcgatc tgctgcacac ccaactcaag
     6541 ctcgccgcag gccagtcgct ggccgacctc ggcctgcagc aaccaccgcc gcttaatggc
     6601 tgcgccgtac aactgcggat caacctggaa agcatgagca gcgatggcag cacccgcccg
     6661 gccgccggca ccctgacggc ctaccaaccg cccagcggcc ccggtctgcg cgtggacggc
     6721 tacggttacg ccggctatcc ggtcagcccc agctacgact cactgctggc caaactgatc
     6781 gcccatgcgc cggattaccc cagcgcactg cgccgcgcct atcgcgcact gtgcgaattc
     6841 cgcttggaag gcgtagcgag caatctgcat ctgctgcaga acctattacg cctaccgcaa
     6901 gtgacgagct accagctgac cacacgcttt gtcgaaaacc acctgggcga gttgctggct
     6961 gcgcaaaccc aagcccaccc gcatcacttc tttgctggta cccagaccgc cagccagagc
     7021 actgagcagg tacaggatgc gccgccaggc tgcctgccgc tgctgaccca cagcaccggt
     7081 gtggtagtca gcctggaggt ggccgagggc gatgcggtgg ccgtcggcca gaacatcgcc
     7141 gtgctggaag cgatgaagat ggaattcgtg gtcagcgcca gccacagcgg cattgtccgc
     7201 caactggcgg tgcaggtcgg cagcgcgctc aatgaaggcc agccgttgct gtttatcgaa
     7261 cctgccgagg tcaacactgc cgccagccag agcgaacaga gcctggacct cgatcatatc
     7321 cgcgcggatc tagccgaagt gctcgaacgc catgcgctga cccgcgacga gcgccgcccc
     7381 caggccgtgg ccaagcggcg caagactggc caacgcaccg ctcgcgagaa cctggcggac
     7441 ttgctggatg acgacagctt tagcgaatac ggcgcgctgg cgattgccgc gcaacgccgc
     7501 cggcgttcac tggaagagct gatcgaacaa agcccggccg atggtctggt ggccggtatc
     7561 ggcacggtca acgccgaggc gttcggcagc caggccgcgc gctgcatggc catcgcctac
     7621 gactacacgg tattcgccgg cacccagggc gtgatgaacc acaagaagac cgaccgcatg
     7681 ctcggcctcg ccgcgcagtg gcgcttaccg ctggtgctgt ttgccgaggg cggcggcggc
     7741 cgaccgggcg atagcgattt cgtcggcgtg gccgggctgg actgccatac ctttgtcggc
     7801 atggccaagc tctccgggct ggtgccgctg gtgggcgtgg tgtccgggcg ctgctttgcc
     7861 ggtaacgccg ccctgctcgg ctgctgcgat gtgattatcg ccacggagaa tgcgaccatc
     7921 ggtatggccg gcccggcgat gatcgaaggc ggtggcctgg gcagctttac cccggagcaa
     7981 gtcggcccgg tcagcgtgca gggccccaat ggggtgattg atgtgctggt ggccgatgaa
     8041 gccgaggcgg tgcgggtggc caagcagtac ctgagctact tccagggcac gcttagcgaa
     8101 tgggactgcg ccgatcagcg tgagctgcgc catttaatcc cggaaaaccg cctgcgggtg
     8161 tatgacattc gccaagtcat tagcactctg gccgacacgg attcggtgct ggaactgcgc
     8221 cggcagttcg ccccagggct gatcaccgcg tttatccgca tcgagggtaa gccgttcggc
     8281 ctgctagcca ataaccccgc gcacctgggc ggagccatcg atgcggtcgc cggcgacaag
     8341 gctgcgcgct ttatgcagct gtgcgatgcc ttcgatatcc cgctgctgtc gctgtgcgac
     8401 acccccggct ttatggtcgg ccctgaggca gagaagcaag ccacggtgcg ccatgtctcg
     8461 cgcatgtttg tcgccgccgc cagcctgagc gtgccgtttt ttaccgtggt gctgcgcaag
     8521 ggttacggcc tcggtgccca ggccatggcc gccggcagct tccattcgcc gctgtttacc
     8581 atcgcctggc ccagcgccga gtttggcgcc atgggcctag aaggtgcggt gcgcctgggg
     8641 tttgccaagg aactggcggc tatcgaagag cccgccgcgc gtcagcagct gttcgacaag
     8701 ctggtggcta aggcctacca gaacggcaag ggcatcaata tggccagctt tctcgagatc
     8761 gatgcggtga tcgaccccgt ggaaacccgc tcctggctgc tgcgcggact gaatgccacg
     8821 ccaaagccgg caacccgtga aggtaagaag cgcagcgtcg atacctggta atccgcagga
     8881 tgccggactg gccgaaccat gtttgggcag tttccggcgc ttcgggtaaa ctgccgcatc
     8941 ttttacacct ataacgatct gcctgatgcc gacgaccttc cacgagattc cccgtgagcg
     9001 cccgctgacg cccctactag accgtgccaa cacgccggac gagctgcgcc gcctgggcga
     9061 agccgagctg gaaaccctgg ccgacgagct gcgccagtac ctgttgtata cggttgggca
     9121 gaccggcggg catttcggcg cgggcctcgg cgtgatcgag ctgaccgtgg ccctgcatta
     9181 cgtcttcgac accccggatg atcgcctggt gtgggacgtc ggccatcaag cctacccaca
     9241 caagattctc accggccgcc gcgaacagat gggcagccta cggcagaagg acggcatcgc
     9301 cgccttcccg cgtcgttcgg aaagcgagta cgacaccttt ggcgtcggcc actccagcac
     9361 cagcatcagc gccgccctgg gcatggccat cgccgccaag ctgcagaaca gcaagcgcaa
     9421 ggccgtggcg gtgatcggcg atggcgcact gaccgctggc atggccttcg aggccctgaa
     9481 ccacgccagc gacgtcaacg ccaatatgct ggtgatcctc aacgacaacg atatgtcgat
     9541 ctccaagaac gttggcggcc tgtccaacta cctggccaag attctctcca gccgcaccta
     9601 cgccagcatg cgcgaaggca gcaagaagat cctctcgcgc ctgccaggcg cctgggagat
     9661 cgcgcgcaag accgaagagc acgccaaggg catgctggta cccggcaccc tgttcgagga
     9721 gttgggctgg aactatatcg gcccgatcga cggccacgat ctgccgaccc tgctggccac
     9781 cctgcgcaat atgcgcgacc tcgacggccc gcagtttctg catgtggtga ccaagaaggg
     9841 caaaggcttc gccccggccg aggtcgaccc gattggctac cacgcgatca ccaagctgga
     9901 gccgatcaac gcgccagcag cacaacctaa gcaagccagc gggccgaaat actccagcgt
     9961 gttcggtcaa tggctatgcg atatggccgc gacagaccca cgcctggtcg gcatcacccc
    10021 ggcgatgaag gaaggctctg atttggtggc gttcagtgaa cgttacccag agcgttattt
    10081 cgacgtagcc attgccgaac agcacgccgt aaccctcgcc gccggcatgg cctgtgacgg
    10141 cgccaagccg gtggtggcga tttactcgac ctttctacag cgcgcctatg accagctgat
    10201 tcacgacgtg gccgtgcagc acctcgacgt gctgttcgcc atcgaccgcg ccggcctggt
    10261 cggcgaagac ggcccgaccc acgccggcag tttcgatatt tcctacctgc gctgcatccc
    10321 cggcatgctg gtaatgacgc cgagcgatga aaacgagctg cgccgtatgc tgaccaccgg
    10381 ccatctgttc gatggcccgg cagcggtacg ctacccacgc ggcagcggcc cgaatgccac
    10441 catcgagccg gggctgaagc cactggaaat cggcaagggc atcgttcgtc gccaaggcag
    10501 caacgtagcc ctgctggtgt tcggcgtgca gctgagcgaa gcgctcaagg ttggcgaagc
    10561 actggatgcg acggtggtcg atatgcgctt tgtcaaaccc ctcgacgaag ccctggtgcg
    10621 ccagctggca gccagcaacg agctgctggt gaccatcgaa gagaacagcg tgatgggcgg
    10681 tgctggcagc gcagtcagcg agttcctcgc ttacgccggc atcctcaagc cggtgctgca
    10741 tctgggcctg cccgactatt acgtcgagca cgccaaaccg gcgcagatgc tcgccgaatg
    10801 cgggctggat gccgccggta tcgaagctgc catccgcgcg cgtctcgcca gcctttctgc
    10861 atgaataccg ctcatccata gctgagccgg gcttgcttgt aattcactct gccgcacctt
    10921 aaggtgcgcg acgttttatc tgtccgaggt cgctgccaaa ccagcagcgt gaaaagggaa
    10981 gcaggtgcgc cgctgtcgct tcgacagccg caagacctgc gctgcccccg caacggtaac
    11041 cgacagcgcg taagcgcagc ctttctcaaa gaccactggg tgcgcctggg aaggtggaaa
    11101 aggcagtcgg cagcccggag acctgccttg gcatgaagtg actggtgttg cggagggcat
    11161 caccgtcaag cgctggctat cgcctccttg cccggtgctt gtccctgccc tcctcgaaag
    11221 ttttctgcct tttgaggatt ctctacgtga aattgtcccg tcttgccctg gctgtggcgc
    11281 ttgcccccag tatcagcttt gctgccagcg ataccagcag ccacgagctg ccaccgctgg
    11341 tcattacccg cgccacgccg ctgaaagcac cggcgccggc cagcgtcaaa gtgatcaacc
    11401 gcaagcagat tgaagaaact gcagcctcat cactccttga tgtactgcgt agtcaggcgg
    11461 gtgtgcagat acgcgacacg attggcgatg gcagccgagc caccttcagc ttgcgtggct
    11521 ttggtgagaa cgcggtgaac aacaccctga ttctggtcga tggccgccgc ctcaaccaac
    11581 ccagccaagc cggagccgac ctcaacagcg tgccattggc caatatagag cgcatcgaga
    11641 tcatccgcgg tgccggcacc gtgctgtatg gcgaccaggc ggtgggcggc gttatcaata
    11701 ttgtcacccg caccccaagc agcaatgagg gctatctcga agccagtcgc ggcagccatg
    11761 acctggaagc ctttcgcggc catatttacc agcaactcgg tggtggattt tcgacctacc
    11821 tgaacggcga gtcgcgtact gctgacaact atcgcgacca taacaacgcc agctacagca
    11881 acgcattcgc ccgcctgcgc tatgaccatg aaagtggcca tgctctgtac gagtaccaaa
    11941 cggtcgacga cgagctgctg ttgccaggct caatcccact agcgctgcgc cgcgccgacc
    12001 gcaaacaaag cagttcaagt gactggagcg acagcaagac ccaggtgcat cgctttgccg
    12061 tcgagcagcg cctaaatcag gtctggagcg gcaacttcga ttacagccag agcgaccagg
    12121 atgccgtagg tagtttcttc ggcagcgact tcgatcagga cacccgcagc gcaaccctca
    12181 gcccacgtat caccgcccgc ttcgataaca acctgggccg tgcagaatgg ctgctggggc
    12241 acgaccatat caccagcgat taccaggcca cctggggcac cgacttccac cagaccctgc
    12301 gcgactggta cagccagtac agccagaacc tcggcagcga cttgaccctg accttgggct
    12361 accgcgccag cgaagcggaa gaccgcaata aaagcgccgg caagacccat accgaccggg
    12421 aagacagcag cagcatcggc ttgagttggc aggccaacca gcagacccgg ctgtttatca
    12481 aacgcgagga tgtgctgcgc tgggccaacg tggatgacaa cggttttgtg ctacccggcg
    12541 tcgagttcct cgagccgcaa accggcgttt cctgggaaag cggtatcgaa tggcaggaca
    12601 gcgtgcagcg ctaccaggcc actctctatc gcctcgacct caaggatgag ctgatgtacg
    12661 actcgcttat tgccaacccc aggccatggg acgccaatgc tcaaggggcc aatatcaatc
    12721 tggacaagac ccgccgcgat ggtctgttgc tggaagccga gcgccaattg agcgagcgcc
    12781 tgagcatcgg cggccaatac agctataccg atgccgagtt tcgcgcaggc tccttccaag
    12841 gcaacaaagt gccgtgggta gcgcgcaaca cagccagtgc ccacctcagt tacctgatcg
    12901 tgccggggct gcgcagctat ctggaagcgg tacataccgg cagccgttat tactctgggg
    12961 acgatgccaa tagccaacac aaggctggtg gttacaccct gcttaacgct gcgctgagct
    13021 acgactacca gcagttcaac agcaagttgc gcctcaacaa cctcaccggt aaacgctatg
    13081 acgcatttgc cagcgcaacc agcagctatc ccgcagcaga agaacaggta caactcagcg
    13141 ttggttaccg gttctgaaat atcggccctg ggtctttaaa ccccagggcc gacttagcgc
    13201 gcctggccca accgctcgca cagcttttcc accgccccca gcatctgaaa actcggtcgt
    13261 tccaggcctg catccggcac cagccacagc tgcttacgct caaccgccgc cacgcttggc
    13321 cagatctgcc aggcgtccag ctgaccttga acgccagcca gaatcacctc ggggttcgct
    13381 tgcagcaccg cttccacgct gacctgcggt gccggcaagg tgagttcggc aaaaatattc
    13441 tcggcaccgc agatacccag agcgtcgctg atgatctgct gaccgccgat ggtgtacagc
    13501 ggcgtatccc agacctggta gaacaagcgc aatggctgtt cacgctgata gcgctgacgc
    13561 aacgcaatca ggccgttatt gaattgctga tgcagctgcc ggccctgctc ggcgcggccg
    13621 atacgcgtgc cgatttcgac gaagttctct gccagatcag ccagcttgcg tggctcgaca
    13681 atcagcaagg gaatccccat acgttgcagc tgctggcgtt gcgcgctgct gatgctgtcg
    13741 ggccagagca gcaccagatc gggctgcagt ttaagcaggc tttccagttc aagctggcca
    13801 tagcggccca ctgaaggtaa ttgcgctacc gctggcggac gctccccgcc atcgagcagg
    13861 ccgaccagca gatcgccagc gtccagctcc agcatgattt cagtcaggga aggcgccagc
    13921 gtcaccaccc gctctactgc cgctgcgggt aatgccagca acaacagcaa acagagagtc
    13981 gcggcgcgca tcagcaggcc acttaagaac gttaccgagg acgccgaggc aaggcgaaaa
    14041 cggtcgagac acgagcgcag cgaagtaaca gccaacggct ggcccaaagg gcgagcacag
    14101 cgagttaagc ggagtttact gctgtaaatg agcatttcga gaccgttttc aacgcagtat
    14161 cggctacgca ggtagttctt cagcggcctg ttagccgagt tggcgaggaa tgcggtagag
    14221 atacaacaga acgatgctgg aaatcgccag aaggatcagc ggaatcgcct ccaggccggc
    14281 gaataccgca acggcagcaa tccaggccgg cagcgccgcg ccgagaaagg ccaggcggcg
    14341 cacacgcgcc agctccagcc aggcatcgtg ttcatctgct gtatcaaggg ctttttcagt
    14401 ggccaccagc gcgcgtttgt aggcgctgaa cagcggcagg ctgacaaaca tcgaagccag
    14461 gcctgcgata aacagcggca tggtcagcag gccagttttt tgctcggcac caaacagcag
    14521 gttgagaaca aatagcggca ccagggccac ggccagctgc ctccaccact ccagcgccag
    14581 ccgccggcgc acttcgccgc gcgtcacagg ctttcctcaa cctcgccctg gtgcaggctg
    14641 ccgagcatat gcccgagttt gccggccttg gtggcgaggt attttttgtt atgcgggttg
    14701 tgatcgatct gcagcggcac gcgggtcgcc acattgatgc ccatatcgcc cagcgccttg
    14761 accttgcgcg ggttgttggt catcagcttg agctggctga ttcccagatg atcgagcatc
    14821 ggcaggcaga tcgcgtagtc acgctgatcg gcgccgaagc ccaggcgctc attggcctcc
    14881 acggtatcgg cgccaccgtc ctgcaactca taggcgcgga tcttgttgag caaaccaatg
    14941 ccgcgacctt cctgacgcag atagagcaga acgccgcggc cttcattggc aatcgcacgc
    15001 agcgccgctt cgagctgaaa tccgcaatcg caacgcaagc taaacagcgc atcgccggtc
    15061 aggcattcgg agtgcaaacg gcccaatacg ggcgcgccat cggccacatt gccaaaagtc
    15121 agggcaacat gttccttacc agtggcttgc tcaaggaagc catgcatggt gaatacacca
    15181 aagggcgtag gcagctggga ggcggcgaca aacacgacgg acaccggatg ctcctaaaga
    15241 aatgggcagg gaaaatcagg ccggcattgt aacagcaggc cccgacagcc gcttagcctg
    15301 aattactgat gataaggatc aagccaacgc gagatgacca aagcgctaat acttggactc
    15361 cagcaccagt acatcctgct ccaccttcca gccgacacga ctgaggaagc tcatgccgag
    15421 caaggcctcg gtaggcgagc cgccttccag caccacggcc tcgacaccca acacttccag
    15481 ttcaccgatc ttgacccgat ccaacgttac gcgccaaccg cgagcagtac cgctggcggt
    15541 attgacctgc aagggcgcgc cgaccactcg gtaatcgatc cccaggcggc gtgcatgctc
    15601 gtcattcaag gccaccgagg tggcgccggt gtccacaaga aactggatag actgaccatt
    15661 gatcgaaccc gccacccagt aatggccgcc gatgcctttg gccacactga gctgcttctt
    15721 ctgcgcttcg gcaaagcctt cgctgtactc gcgactcagg ctgtagctgc gctcgacacc
    15781 gtcgacacgc aacaccgcgc cacggctgtc ggcactgact accacaacac cgccggggcc
    15841 gacctggcca accttgacca gtttgcgctg accatcgaca ttcaacaccg cagcacccgg
    15901 aaacagaccg acgacccgca cctggggagc agcccagacg caactggcgg ccagcagcag
    15961 ctggcccaga atgaaacact tgataccctg catacggctt ctcgtggcgg ttaatcgaag
    16021 ggatagggct gctgccaacg ggtaaaaagc tcgcgcaaac gatcctctgc cagcaacagt
    16081 tccattcgcc ggtcgtagag ttcggccagc gcccggcccc gtggattatc ggcaaaaccc
    16141 agatagagcg gcagcgcggt caggatggtc gagcggtaac gttcctgagc cctggtcgtg
    16201 gccagcagat cgtcaacctc gctcagtgca tcgatataga aatcggcatg gccaaggtca
    16261 agcatgccca gaataccgct acggcgctgc acttcgcggt actggcgcac gtctggcaga
    16321 tagcgctggt actcataacc gcggatccac accagacgaa actcgccgat gttggccaga
    16381 ctgggcagcg gcttatctgt cagaccgagg gcggcaatcc gatcggcatc gtagggaagg
    16441 ctcgggtaga acaccccgtc gtcgacttca ttgaggtaag agccgaccca ggcatccgcc
    16501 tcgccgcgct gcaccaggcc gatggaacgg gtgtaaggca cgctctggat ctgcaacgcc
    16561 actccggccg gctcgaatac caggcgcatg atgtcccagg ccaggcccgt accgtcggca
    16621 tgggtatgcc tctcccatac ctcgctggca atacgcacct gctccggcat cggggcatta
    16681 gcctgggcgg tgcaacaggt cagtagagcg aagagcaaga tcaaacatct gcggcccata
    16741 cgccacctcc taatgatcag cccaaccaat gatcaaccca acaatgccgc cgccacaccg
    16801 ttggcccagc cccagacaca caactgcagg gccatccagg cgaacacccc tgccagcacg
    16861 tcatcgagca tgatgccgac accgccatgc acatgccgat caatccagcg aatcggccag
    16921 ggcttgagga tgtcgaacag gcggaataat agaaagccta gcagcagcca gccccagccg
    16981 tccggcacca gccagagggt gatccacata ccgaccattt catcccaaac aatgccttca
    17041 tggtcgtgca cgcgcaggtc atcggctacc ttgccgcaca gccagaagcc gaacagcatg
    17101 gtgatgccga gcatcagcca gtagccccag tccggcagca tctgcaacag cggaataaac
    17161 ggcagcgcga ctagcgagcc ccaggtgcct ggggctttcg gcaaagtgcc agagccaaag
    17221 ccgaatgcga gaaaatgaca ggggttgcgc cagaccgaag gtggcacaaa ctcggcggga
    17281 acttgcttgg gatgatcagt cactggcact tccaaaatgt tgatagccgc tgcgtggcgg
    17341 ctgcacgggc tggccttgcg agtccagcag agtaacgccc tggccggcca caacacgacc
    17401 aataactcgc acaggccagc cagcatgctg caaatccgcc agatactgcg ccggcagggt
    17461 gaaggccagc cggtaatcat caccaccgct taatgcgcac tgacgcgccg ccgacgcccc
    17521 ggcaaatgcc agcaaggcgg cagacatcgg cacctgtgcc tgctcaatca gcagtgccac
    17581 accggacgct gcagcgatat ggtcacagtc agccaacaag ccatcggaga tatccagtgc
    17641 ggccgtggcc ttgccacgta gcgccaggcc cagatcgagc tgcggtatag gcgaccagta
    17701 gcgcgccagc agcgcctcgg ccactgctgg ctcggcttgg cgctgcccga gcaccagcgg
    17761 caaagcgccg gcggcatcgc ccaactcgcc accgacgcag agcaaatcac cgacctgcgc
    17821 gcccttgcga gtcaacgcca taccaaccgg cacacggccg aacacgctca gggtgaggct
    17881 cagggggcca cgggtggtat cgccgccaat caggcgaatc gcgcagtgtt gcgccatttg
    17941 atccagacca cgggcaaaag cctgcagcca atccggctca gcactgggca gggtcaaggc
    18001 cagggtaaaa ccgacaggcg tggcagccat ggcggccagg tcactgaccg agacgcccag
    18061 ggcacgctgc cccagcagaa aaggatcgga ggattcggga aaatgcaccc cggcaaccag
    18121 ggtgtcggta gagatcgcaa gctgttcgcc agctggcact tgcagcaggg cgcagtcgtc
    18181 gccgatgccc aatgccacgc cttcgccggc ctgcgcacag gacgcagcgg cgaagtaatg
    18241 acggatcagc tcgaactcac ccaaagcagg cttaacgctt gttagcgcga acttcatccg
    18301 cgcgcagacg cggagccagc ttatccagca cgccgttgac gaacttgtgc ccgtcggtag
    18361 cgccaaacac cttggccagc tcgatgcctt cgttgatcac cacgcgatag gggatatcga
    18421 tgcggttttt cagttcgtag gtcgacagac gcaggatcga caattcaacc gggtcgatct
    18481 cagccagcgg acggtcgagc agcgactcga acgcaccgtc gatctcggtc ttctgccgcg
    18541 gcacgccgtg cagaatctcg tggaagtagg caccgtcgac cgcagtgaaa tcgttatcga
    18601 cgcggaactg cgcttcgatt tcgttcagtt gctgccccgc catatgccag gaatacagcg
    18661 cctgcatggc cagagtgcgc gcctgacggc gcgcgagggt cttgctgctg gggccttttt
    18721 tcggcgcctg tggctgctgc ccgttaccgt cggtctggct cacttggcct ccaactgcgc
    18781 cagcaggctg accatttcca gggcggacag tgctgcttcc gcgcctttgt tgccggcctt
    18841 ggtgccggat cgctcaatgg cttgctcgat ggaatcgaca gtcagtacac cgaaggcaac
    18901 cggtacgccg aactccatgg acacctgggc caggcccttg gtgcattcgc cagcaacgta
    18961 ttcgaagtgt ggagtgccgc cacggatcac cgcaccgagg gcgatgatgg cgtcgaaatc
    19021 gccacgctgg gcgactttct gcgcgaccaa cgggatttcg aaggcacccg gcgcgcggat
    19081 gatggtgatg tcgctttcgc tcacgccatg gcgaaccagg gcgtcaactg cgccgcttac
    19141 caggctttcg acaacgaagc tgttaaagcg gccgacgacc agtgcaaagc ggcccttggg
    19201 ggcgataaaa gtaccttcga tggtcttcag ggtcataacg cgtctcgtct atttaaagag
    19261 ccgggccgcc agtggcgacc cttgggatta tttggagggc aggtattcta caacttccag
    19321 gtcaaaaccg gatatcgcat tgaacttcat tggcgaactc atcaggcgca tcttgcgcac
    19381 accgaggtca cggagaatct gcgaaccggc accgacggtg ctataggtgg tcgggttggc
    19441 cggttgttga cggtccaaca gggccagcag ttcaggcccg gtcagcggat tgcctagcag
    19501 cagcaccacg ccgctgccat ccttggccac ttcagccatg gccgcacgca ggctccaacg
    19561 gcccggctga ttgaccagaa acaaatcgcg cagcggatcc atgttgtgca cccgcaccag
    19621 ggtcggctct tcagcgcaca cagtgcctaa ggtcagggcc atatgcgccg tatcctcgac
    19681 gccatcgcga taggtcacca agttgaactg gcccaactcg gtgtcgagca cctgctcgct
    19741 gacacgctca accgtgcgct catgcatcaa gcggtagtga atcaggtcag caatggtgcc
    19801 gatcttgata ccgtgctcgg cggcaaacac ctccagctca gggcgacgcg acatggtgcc
    19861 gtcgtcgttc atgatctcgc agatcacccc gctcggctcg aaaccgccca tgcgcgccag
    19921 gtcgcaggct gcttcggtgt ggccggcacg cgccaaaacg ccaccaggct gagccatcaa
    19981 cggaaagata tgcccggggc tgacgatatc gtcggctttg gcgtttttcg cagcggcagc
    20041 ctgaacggta cgcgcgcggt cggcagcaga tataccagtg gtcacccctt ctgcagcttc
    20101 gatggagacg gtgaacttgg taccgaaacc ggaaccgtta cgcggcgcca tcagcggcag
    20161 cttgagggtt tcgcagcgct cgcgggtcat cggcatgcag atcaggccac gggcgaaacg
    20221 cgccataaag ttgatatgtt cggcggtgac acactcggag gcgatgatca gatcgccttc
    20281 gttctcgcgg tcttcgtcat ccatcaggat gaccatcttg ccggcgcgga tgtcttcgac
    20341 cagttcttcg atactgttaa gagccatgct ggcgattcct tatttgttca ggtagccgtg
    20401 ttcggcgaga aaactttccg tcatgcccga ggctttcggc tcggcggcct tgtcaccgag
    20461 cagcagacgc tccaaataac gagccagcag atccacttcg aggttgacct gactgccagg
    20521 gcgatagtcg accatgatgg tctcagccag ggtatgcggc acgatggtca gctcgaattc
    20581 agcgccgttg accgcattga cggtcaggct ggtgccatcg acggtgattg agcctttgtg
    20641 ggcgatgtac ttggccagct cgcgcggcgc gcgaatggtg aactgcacgg cgcgggcgtt
    20701 atcggcgcgg gcgatgactt caccgacacc atccacatgg ccactgacca agtggccgcc
    20761 aaggcgactg gtaggcgtca gcgccttctc cagattgacc ttgctgccgg ctttcaagct
    20821 gataaaagcg gtatgcgcca gggtttcgcg gctgacatcg gcccagaaac catcgcccgg
    20881 cagctccacg gccgtcaggc atacgccgtt gaccgcaatg ctatcgccga gtttgacgtc
    20941 ggcgagatcc agcttgccgg tcgccacata cacccgcacg tcgcccccct tgggcgtcag
    21001 tgcgcggatg ctgccaatcg attcgattat gccggtaaac atgccacccc ctgcgtcgcg
    21061 cccacggcaa agcactggcg aacaacgcca gaagcccggg gcacggaata aaaatcaaac
    21121 atctaaacca actcctgttt tataaaaaac agggcgcgtt tgatcgatag ggatttcgag
    21181 gcacgcctgg gcgcgccttg ttgaggcaat cccgctctct ctttatccgg actttaaccg
    21241 tcggccccgg aatcacaccg ggtctgctga ccttgccaag cggtttacac cacctggcaa
    21301 gcgctcgcgg gctaggcctt gcagccatta ccgccggtgg ggaatcacac cccgccctga
    21361 gaacgttacg gctacttgcg cagccgagac gcgttttacc acaaattgcc gacgccctgc
    21421 atcggcaaac gacaggatga atggcgtgcc atcgctaatg actagtcatc gctcatggcc
    21481 gcccatcaaa cagccggctg tggcactgcg ataatccgcc agtcatcacc gactgcgcgc
    21541 atctcgacga ttttcagcgc cggcgcatcg gccatccgtg ccaacggcaa gtcgagcagc
    21601 ggccgcgcac tggaaccaag cagcttgggc gccacgaaca gctggtactc atccaccagg
    21661 cccaaacggg caaaggcccc agccagacgc ggcccagctt ccaccagcac ttcgttggcg
    21721 ccacgattgg ccagttcgac cagcagccga cgcagatcaa catgcccgtt actgccgggc
    21781 acggccaata gttcgtgtcc atcatcgaga tagcgatcac gcgccgacgc tgctgcgcag
    21841 gtcgcgacca tggcggcacc agcctgaaag aacggtacgc tcaacggcac gcgcaaacgc
    21901 ccatcaacca gcacacgcaa tggtggccgg gcctgcgcca aggcacttag ttcggcgccc
    21961 aggcccagtt catcagcgcg cacagtcagg cgggcatcat cggccagcac cgtgtcagca
    22021 ccggttaaca ccacactgga gcgcgcacgc aggcgctgca ccgcggcgcg tgcagccggc
    22081 ccggtaatcc actggctttc gccgctggcc atggcggtgc gcccatccag actcatcgcc
    22141 agcttgaccc gcacaaacgg cagtcctttc tccattcgct tgataaaccc ggcattcagc
    22201 gcccgcgctt cggtttcaag tacgccgctc tgcaccgcga taccggcatg catcaaacgc
    22261 agcagaccat caccaccgac ctgtggattg gggtcctgca tggcggctac gacgcgggta
    22321 ataccagcat tgatcagcgc atcagcgcac ggcggcgtgc gcccgtggtg gctgcaaggt
    22381 tctagggtga cgtaggcggt ggcgccgcgt gccttatcac cggcctgacg cagtgcatgt
    22441 acttcggcat gcggctcgcc tgcgcgggca tgccagcctt cgccgacgat gcgcccatca
    22501 cgaacgatca cgcagccaac gcgcggattg gggtgggtgg agtagaggcc tttacgggcc
    22561 agctccagcg cgcgagccat aaaggcgtga tctgagttca tcgtcaacct ttggcaggtt
    22621 cgcgggacag gcggtcgatt tcctcacgga actcgttgag gtcttgaaaa cgtttgtaca
    22681 ccgaggcaaa gcggatatag gccacttcat caagcttctg tagctcgccc atcaccaact
    22741 caccaagcac cagcgacttg atctcgcgct cgccagtggc gcgcagcttg tgcttgatat
    22801 gggcgatggc cgcttccagg cgctcgatgc tcaccggacg cttctccagc gcacgctgca
    22861 tgccggcgcg cagcttgtct tcatcgaagg gctggcggct accgtcctgc ttgatcaggc
    22921 gcggcatcac caactcggca gtctcgaagg tggtgaagcg ttcctcacag gccaggcatt
    22981 cacgccgacg acgcacctgt tcaccctcgg caaccaggcg cgagtcgatc actttggtgt
    23041 cattggcgcc gcagaaggga cagtgcataa ataaggccgg ctttagaacg aagatagcca
    23101 tggtagcgca tcccgcaggc aagacaagcc gaggggtttg cggtatacag gcgcccatgc
    23161 aaaccagcgg agttgataca cccgatgaaa gtccctaagc cgttgcaacc gctgattcta
    23221 gtgagcctga ttgccttact gactgcctgt gccagcgaca gcccgccacc cgccaaaccc
    23281 acaacacccg cgcctatagc ggccccggca gaagctgcgc ccctgccggc ctatcagcgc
    23341 gagctgagcg gcagcttgtt gaatgtgccc aatgaagcgg atgtcgagct ggccctgctg
    23401 gcggtcgacg agcgcggccg gccgcaggcg ctgctgggca atatccagct gcgcggcacc
    23461 ggtaacccgc tggcgttccg cctgccgttc aacccggaaa cctttaacaa gcacagccgt
    23521 atcgagctgc atggccgcgc caaccaagcc ggccggctga ttctgcgcct gcccgagcaa
    23581 ccgattgccc gcggcgaaac ccaggccctc ggtgagttgc gcttggtgcc tgcaccatga
    23641 cagccccggc tgccctgcag gctgcgctca gcgaactgct gggtgacgct cagctccgcg
    23701 cgcaaaccct gcccggcacc gcgatcaaac tgtggctgat cgacccggcc aacatggatc
    23761 gctgtttcag cccggaagaa acccgcctga tcctcgaaga accaccctac tggtgtttct
    23821 gctgggccag cggccttgtc ctggcgcgct ggctggccga gcaccccgag tgggtgcgcg
    23881 gcaagcgcgt gctggacttc ggtgcggggt ccggcgtggc ggcgattgcc gcagccaaag
    23941 ccggcgcgct ggaagtggtg gcctgcgacc ttgatccgct ggcgattgct tcctgccagg
    24001 ccaatgccgc actgaatgat gtgcagctga gttattccgc agattttttc gccgaggctg
    24061 atcgcttcga cctgatcatt gtcgccgacg tgctctacga ccgcgccaac ctgcccttgc
    24121 tcgatcagtt tctcagccgt ggccgtgaag cgcttgtggc tgactctcga gtgcgcgatt
    24181 tccaacaccc gctatatcag cgcctcggca tattggaagc ctgcacctgg ccggatctgg
    24241 ccgagccggc tgagttccgc catgtcagcc tgtaccacgc agagcgtgcc tgaacgcctc
    24301 ttgtatccag cacccgcgcc cacatttata gttcatcgca ttgcagccca tcacaacttg
    24361 cgcccagccg agacagacca tgagcgactc cccttacatc tttgatatcg caggcagcgc
    24421 cagcttcgaa cagctggtga tcgaaaattc cttccagaag ccggtgctgg tggatttctg
    24481 ggccgactgg tgcgcgccgt gcaaggcgct gatgccgctg ctggcgaaga tcaccgagga
    24541 atacaacggc gagctgctgc tggccaaggt caactgcgat atcgagcagg acatcgttgc
    24601 tcgcttcggc atccgcagcc tgccgaccgt ggtgctgttc aagaatggcc agccggtcga
    24661 cggctttgct ggcgcgcagc cggagtcggc gattcgcgaa atgctcaaac cccatgtcgc
    24721 cgaaccggca cctgcggcgg ccgacccgat ggaagccgcc caggcactgt tcagcgaagg
    24781 ccgctttgcc gatgccgaag ccgtgctcaa ggtgctgctg accgagaata acgaacacgc
    24841 cgccgcgctg attctctacg cccgctgcct ggccgaacgt ggcgagctgg gcgaagccga
    24901 agcagtactg ggcgcagtaa aaggcgatga acataaacag gccctggccg gcgcccgcgc
    24961 gcagctgacc tttctacgcc aggcagccga actgccagat gttgccagcc tgaaaacccg
    25021 cctggcgcag aacgccgacg acgatgaagc gagctaccag ctagcgatcc agcaactggc
    25081 gcgccagcac tacgagccgg cgctcgacgc cctgctcaag ttgtttatcc gcaaccgcag
    25141 ctacgccgac ggcctgccgc acaagaccct gctgcaagtc ttcgacctac tcggcaatga
    25201 ccacccgctg gtcaccgcct accgacgcaa gctgtatcag gctatttact gatcgcagcg
    25261 acccgcccaa cggcacggct gtaatgttgg gcttcgcagg ctcagcacca acctacgctt
    25321 acttgtcgac ccagcaggcg tagggcgggt gcaacccgcc gtgatgtgtc gccacttaca
    25381 actctggcgg gtttcacccg ccctacgggg tacggctgca ggcccggcca acgtggcccg
    25441 gcccaaccta cgcctactcg ccggcccagt gatagatagg cgcatccacg cccgtttcca
    25501 tccgcacctg cgcgcaatga cgcaggcgca ccagcaggcg cttgcctgcc gcctcgccac
    25561 ccgcaagccc tgcgagttgc ccaagcaatt gcggcccttc gatctgccca gcttggcgca
    25621 acagctccag ggtagtttgc cacaaggcat cgttggccac tggtgcggca gctgctgccg
    25681 gcgctggcgc tacctcaaga ggaatgtgct gggccagctg cgcccaatcc tgcgcatcca
    25741 gttcaaccgt caggtccacc ggccaggcgc caatctgccc acgaattcgc accatttttc
    25801 tgccctcaac tgatcagctg ccatgctccc acgtccgcag cgggccgcca aggaaggctg
    25861 gtcagggcca ccataaagtt gttataacgt aacgcattat ttatatcccc ggagactgcc
    25921 atgcgccgcc tgctatttgc cctacctttc gccctgctgc cacttgccgc cattgctgct
    25981 gaacacagcc acgaccacga tcatgatcac gccgaacaca gcagcctgga tgcccatgag
    26041 catggttcgg cgcgactcaa tgtggtgctc gacggccagg ccctggaact ggagctggaa
    26101 agcccggcca tgaacctggt gggcttcgaa catgccgcca agagcgacgc cgataaaacc
    26161 aaggtcgcag ccgcgcgcag ccaactacaa gacccgcagg ccttgttcgg tctgggtgcg
    26221 ggcgattgca ccctgagcga aactgagctg gaaagcccgc tgttcgaaga cgacgagcac
    26281 gagcacgaag aacatcacga taaagacagc gagcacagcg aaatccatgc gcattaccat
    26341 ctcgactgca aaaagcccgg cgaactgcag caactgaacc tggccgagct gttcaagcgc
    26401 ttccccgcca ccgagaaaat tcaggtacaa ctgatcggcc cgaacggcca gcagggcctg
    26461 gagctgacgc ctgcacagcc taccctgagt ttctaacctc gcagcacttg taggagccag
    26521 cttgctggcg atcagcgaat caaaagcatc gtcggattgc cgaaccacag caagctggct
    26581 cctacaaaaa gcacaagagc agccaccaag caaaggtagc gcgcccctaa acgagttatc
    26641 catgagttca gccctgatcc aactgtccaa cctcggcttt gcctggcccg gccagcccga
    26701 gttgctggat atcgacagtt ttaccctgca aagtggcgaa agcctttttc tcaaaggtcc
    26761 cagcggcagc ggcaagacca ctttgctggg cctactcggc ggggtgcaga agcccaatcg
    26821 cggcagcatt caactgctcg accaggacct cagcaccctg tcggccagtg cacgcgaccg
    26881 tttccgcgtc gatcacaccg gctatatctt ccagcagttc aacctgctgc cgtttctctc
    26941 ggtacgcgag aacgtcgagc tgccctgtca tttctccaaa ctgcgcgcca gccgcgcgat
    27001 gcaacgccat ggcagtgtgg ctcaggccac cagcaccctg ctcgcccatc tggggctgaa
    27061 tgaggcaagc ctgcttgagc gccgcgccgg cgacctgtct atcggccaac agcagcgcgt
    27121 cgccgccgcc cgtgcactga tcggccagcc ggagctggtg atcgccgacg aacccacctc
    27181 ggccctggat gtcgacgccc gcgaagcctt tctcgaactg ctgttcgccg agtgccgcga
    27241 ggccggctcc agcctgctgt ttgtcagcca cgatcaaagc ctggcgcggc tgttcgaccg
    27301 cagcctgtcg ctcgccgagc tgaacaaaac cacccgcctg gcggagattt aacgatgtat
    27361 ctactgcgtc tcgccctggc cagcctgggc aaccgccgct ttaccgcctg gctgacggtg
    27421 ttcgccatcg cgctctctgt gtgtttgttg ctcgcggtcg agcgggtgcg taccgaagcc
    27481 cgcgccagct ttgccaacac catcagcggc accgacctga tcgtcggcgc gcgctccggc
    27541 agcgtgaacc tgctgctgta ctcggtgttc cgcatcggca atgccaccaa caatatccgc
    27601 tgggaaagct tcgagcagtt tgccaagcac cgtcaggtga aatgggcgat cccgatctcg
    27661 ttgggcgact cacaccgtgg ctacagggtg atgggcacca gcccggcgta cttcgaacac
    27721 tatcgctatg cccgcaccgc acaactgcag ctgagcagcg gccgcgcctt tgccgatgat
    27781 ccgtttgaag tggtgctcgg cgcggaggtg gcgcaagcgc ttaactacca gctcaatcaa
    27841 gaactggtgc tggcccatgg cgtggccacc atcagcctgg tcaaacatga tgacaaaccg
    27901 ttcaaggtgg tcggcatact caaacgcacc ggcacgccgg tggatcgcac cctgcatatc
    27961 tccctggccg gtatggaggc cctgcacgtc gactggcaga acggcatgcc ggcgcgcggc
    28021 gcagccaaag tcagcgccga gcaggcgcga gccatggacc tgcaacccaa acagatcact
    28081 gcctttctgc tgggtctgaa cagcaagatc gccaccttta gcctgcaacg ggagatcaac
    28141 gaatttcgcg gcgagccgct gctggcgatt ctccccggcg tcgccttgca ggagctgtgg
    28201 agcctgatgg gcacggccga gaaggcgctg tttgtggttt cactgtttgt ggtgctgacc
    28261 ggactgatcg gcatgcttac ggcgattctc accagcctta acgaacgccg ccgggaaatg
    28321 gcgattctgc gctcggtggg cgcgcggccc tggcatatcg ccagcctgct gatcgtcgag
    28381 gcctttgccc tggcgctggc cggcgtgctg ctcggtttgc tgttgctgta cctgggtatc
    28441 gccgcggccc agggctatgt gcaagcgaat tacggcctgt acctgccgct gaatctgccg
    28501 acccgctatg agtggacgct gctcggcgct atcctgagcg ccgccctgtt gatgggctgc
    28561 gtaccggcct ggcgcgcgta tcgccaatcc ctggccgatg gcttatcgat ccgcttatga
    28621 ggctttttgc atgcaccgct ctctgctcgc caccctgctg ctcaccctgg ccctgcccct
    28681 ggcggctgcc gaggtgcgcg agctgacctg gtcggaaatg gtcccagccg acgccccgcc
    28741 gcaagtcgtg gagccggcac cgatccacga cctctcgcag ctggccgacg ccctcgccga
    28801 atccggcccg gcggccatgc agcaatcgcc tgccgcgccg gtggtgcagg cgctggatgg
    28861 ccaatcggta aaactgccgg gctatatcgt gccactggat gttaccgatg aaggccgggt
    28921 gaccgagttc ctgctggtgc cctacttcgg cgcgtgcgtc catgtgccgc caccgccatc
    28981 gaatcagatc gttcacgtca ccagcgagct gggtgtgctg cttgatgcgc tgtatcagcc
    29041 gttctggatc gaaggcccgc tcaaggtcga acagaccagc agcgagctgg ccgaggccgg
    29101 ttatcagatg gaggcggaaa agatttacgc ctatgagctg ccagatagct aagaacctat
    29161 ccacgacctg ctgcgcgtcg gccctgctgc gttaaaaaca ggttcggatg cacatgtaca
    29221 actcgtacac tccgcttcct cacctgtttt tgccttacat ggctctagct cgcgagatcg
    29281 tggacagatt ctaggcagcc ccggagacaa aaaagccaga cctaggtctg gcttttttat
    29341 tgggcgctac aaaccggcag tcgactagcc ggcactggcc acgtgttttt cacgctggta
    29401 gctcaactcg ctttccgccc actggcgcca ggcgcgtacc ttgctgcgtt cggcgccagc
    29461 acgttcagcc ttggccaggg cgtcgagccc ggcctgccag cgcgcctgtt ccagctccag
    29521 ctgggccagg tgcaaccagt gcttggcact gcccgagcgc tgcgccagtt cgcggaagat
    29581 ctgcgccgac ttgctgcgct cacgagcttg ccaccaaagc agccccagcc gctcctcacg
    29641 agcgctggtg cgcggcaaca ggccctggtc gagcatcccg gcgagcagct tagcgccctg
    29701 ccagggttga tcggcagccc cggccagcag caccagattg tccagctcgc tttcagtgaa
    29761 gcgcagaccc ttctggtacg ccgcgcgcaa ggttgccagc gatttgtcgt gactgccgct
    29821 cagctgctgc aaggcagcca actgacgcca gccctgggcg ttattcggct ggcgcgcgag
    29881 caactggcgc tgccaacgct cagctgcagc gtacttattc agatcagcgt tcattgcgac
    29941 gagaaactgc aaccagctat cggcggcctg cggattagcc tgcacataac gctcggccaa
    30001 cggcagcgcc ttggccggtt gaccaagacc ctgataggcc tgcaccagca tctgcagcac
    30061 ttcttcattg gcgttggcct gccccggtgc cagcagggtc accacctgag cgtagcggcg
    30121 ctcgaccagg ttcagacggg ccaagttcag ctcttcggcg gcctgcagct cggcgtccag
    30181 cttgccgctc ttcagtacct tttccagcag ctcgatggcc tgggcattct gcccttcggc
    30241 ccaggccaga taaccacggc tgcgccacag cagcgcctct tccagactac ccggcgtgcc
    30301 cttggcagca tccagagcgc ggcgcgcagc cgcgtagtcg cccttcttct gtgcggtctg
    30361 cgccgtatcc agcgcgcgga acacgcccgg atcaacagtc tgcccagccg cctgcacggc
    30421 accagcgcta cacagcgcga tcagcaacag tagacgatac atatcagcga gctccttcca
    30481 gacggaagta cagggttttc actgcttcac gggccaccgc caggccgcct tcagtgcgcg
    30541 gcgcaaaccg ccagcgcaca gcggcgcggc gggcttcgcg ttcgaacacg ttctgcgggc
    30601 tggcttcaat tacgcgcacg ttttctaccg caccggcgcg gttgatggtg aacgccagtt
    30661 tcacgtggcc ttcgatgccg cgctgcaagg cgtagcgcgg gtaatccggg ctgacatcgt
    30721 tgagcggcat cacttcactt tccgggccgc ctggctgacc accggcatcg ctgggcgctg
    30781 gcgcgctcgg cgccgaagcc cccgccgcca gaccagtcag acttggcgtc ggcgcgctat
    30841 tcacggcaat cccgctgctc aggctcggca ctggcagatc cagtgccggc aagttggcgc
    30901 tcggggtagc cgtcatcggc gtaggcggcg tcggggtttg cggcgtttgt gcttgcggcg
    30961 gctgcggcgc ctgctggcga gtgcgtgtcg cggtgtcgct ggagtcgcca tccaggcgga
    31021 cgaaat
//
LOCUS       DASRUM010000154         9826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2243237, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000154 DASRUM010000000
VERSION     DASRUM010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9826
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2243237"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1484)
                     /gene="yegQ"
                     /locus_tag="VFF22_10120"
     CDS             complement(168..1484)
                     /gene="yegQ"
                     /locus_tag="VFF22_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HZX17204.1"
                     /translation="MASTPRYPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRN
                     NEFDHDNLEAGIREAHAQGKQFYVVVNIAPHNAKLKTFLRDLLPVVLMGPDALIMSDP
                     GLIMLVREHFPQMPIHLSVQANAVNWASVKFWKQQGLSRVILSRELSLEEIAEIRSEV
                     PGMELEVFVHGALCMAYSGRCLLSGYINKRDPNQGSCTNACRWQYKAEQAGENELGEV
                     VQIVEPTLGVGAPTEQLFLLEDASRPGEQIAAFEDEHGTYIMNSKDLRAVQHVERLVQ
                     MGVHSLKIEGRTKSHYYVARTAQVYRKAIDDAVAGKPFDKSLMDTLESLAHRGYTEGF
                     LRRHVHDEYQNYQRGSSVSEQQQFVGELTGERRGELAEVRVKNRFAVGDGLELMTPQG
                     NLRFTLDQLENSKGQAVAVAPGDGHVLYLPVPPEVQLEYGLLMRDL"
     gene            1692..2210
                     /locus_tag="VFF22_10125"
     CDS             1692..2210
                     /locus_tag="VFF22_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZX17205.1"
                     /translation="MSAGDSTHQQLVGLFYRDHRDWLLGWLRKSLYSPERAEDLSQDT
                     FVRLLGRSDLDQAREPRALLTTIAKGLLIDHFRRSALERAYLEELGQQPHDVQPSAEE
                     QALALDALREIDRLLGQLSSNARAAFLHNRLDGMGHAEIAERLGVSVSRVRQYLAQGL
                     RQCYIALYGAPQ"
     gene            2207..3160
                     /locus_tag="VFF22_10130"
     CDS             2207..3160
                     /locus_tag="VFF22_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HZX17206.1"
                     /translation="MSAVSAQVLDAAIEWQLCLDSGTVSAEQQHAFAEWLAAHPEHAR
                     VWQQLNGLDIRLASAAAAPARQALLHSARSKGLRRMGGTALGLLLSSALALGLLAQQR
                     PLGDYLADEHTGRGEQRELLLSDRSLLRLNSASAVDIEFSRTQRAVRLRSGEILVQTA
                     HGDTRPFVVLTEHGSLRALGTRFLVRREGNSTRLTVLQSAVVAHPAEGNSERIIQSGE
                     QVLMQRQRLGSVLPAPLSAAAWSQGMLMADNLRLADLLDALGEYRSGYLGLDPSLANL
                     RISGSFPLHDSDKALAALPPSLPVRIERFGNWWTRVVPAAP"
     gene            3399..5759
                     /locus_tag="VFF22_10135"
     CDS             3399..5759
                     /locus_tag="VFF22_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZX17207.1"
                     /translation="MTRALPLFRPGLLAVAISLASVCAPALADSYQLPAAPLASTLTQ
                     IASQAGIVLSIDPALTANKQSAPVTGDYDALGALQQALQGSGLQLQQNSAGTYNLAPV
                     PQAAVALPDVTVTSQVVNEGSAASGYRSDTAQNIGALGGMRLQDAPYSVSVVSKELLQ
                     NIQATSSDDVFKLSPATQFTSPVSAGYASAVSMRGFSGSGNLSIANDGLRFSNSYDGG
                     NFIEEMERLEILTGLSGFLYGPASPGGLVNYVLKRPTYERYNSVTLGNAGGENYYLHG
                     DFGGPIDDAGVFAYRVNVLTQDGETALDLQKRRREMISVALDWNVSDDLQIQFDASHK
                     KTENRGLTSYWYFADQARRPDAKDLDTDKLYSQKWAYADSEHDRIGSRFNWRLDETFT
                     LRGALAYSQYTEEYAYTGPTVENATPIGQYTQPLYAFAPVETEETSGNLFLDAEFTTG
                     SIQHKTTLGYQGNVARVRNYTDAGVYGRNIGISVPFGEQPQIDKPDFSADIGHGDQRL
                     VSRTQSDNLLIGDVITFNEQWSSILGLTRTRIETWSAGINNAETENSPNVSLIYKPQP
                     WLTTYVSYIEGFQAGGLAPWNAVNVNETMAPLVSEQYEVGVKATVGETLLTLALFNID
                     KPNAYINGAGYYVQDGRQENNGLEFGITGKVLPELTLVGGVTLLDPQVKDSGAAASEG
                     QKPTDVASQLAKLYAEYDINAVPGLALTGGTYYTGKQYSDEANQHSLPSFTTFDAGAR
                     YRAQLSSDNTLTLRTNISNLANKEYWLSSHYLGAPRTLTFSAQLEF"
     gene            5820..6950
                     /locus_tag="VFF22_10140"
     CDS             5820..6950
                     /locus_tag="VFF22_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HZX17208.1"
                     /translation="MKSTTIRRWSQVHTWTSLICTLFLLMLALTGLPLIFHHELEHLL
                     GEAPELRELPANTPHLSLQQLVEAAERHRQGEVIQYLGYDEDEPNGVVAITAATAGTE
                     PNSSHTFMLDARSGEAIEMPAANGGILMVILRLHVDMFAGLPGKLLLAFMGLLFVVAI
                     VSGVVLYAPFMRRLKFAEVRHEKATRTRWLDLHNLIGIVTLTWALVVGVTGVISACAD
                     LLISAWRTESLAAMVEPYRDAPPLTQRAPVNDVLTIAAEATPGMQPDFIAFPGTRFSS
                     EHHYSVFMKGNTHLTAHLWTPVLIDARSLDVTAIGQRPWYMDALSMSQPLHFGDYGGM
                     PMKILWAVLDVLTIIVLGSGLYLWWVRRRTPTTQRERAETTA"
     gene            6947..7114
                     /locus_tag="VFF22_10145"
     CDS             6947..7114
                     /locus_tag="VFF22_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010167463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17209.1"
                     /translation="MNWRQSTFALPLLIGLLSAIGLVSALLGDGGWDAVAWLGLGLPA
                     VIGCWPLLRRS"
     gene            complement(7188..7694)
                     /locus_tag="VFF22_10150"
     CDS             complement(7188..7694)
                     /locus_tag="VFF22_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17210.1"
                     /translation="MLWTILAIVVSGLGAAGIAMLLRKLTRNRLPKWIIPAFGGLGML
                     SYQIYYEYSWFEHQLQRQPAESVLVASENGEVFWRPWTFYWPMTTAFTVLDSKSVQRQ
                     DAGGTSIVAFNLYRFEKQHIDHVSHQAHLLNCTTAELLPLDAEKKPLLDQLKAVAKDD
                     ALYRQVCQ"
     gene            complement(7848..9617)
                     /locus_tag="VFF22_10155"
     CDS             complement(7848..9617)
                     /locus_tag="VFF22_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003301492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HZX17211.1"
                     /translation="MSQFVINMLFVGASFALYFGIAVWARAGSTKEFYVAGGGVHPVT
                     NGMATAADWMSAASFISMAGLIAAGGYANSTFLMGWTGGYVLLAMLLAPYLRKFGKFT
                     VPDFIGDRFYSNGARLAAVVCLIIICVTYVIGQMAGAGVAFSRFLEVSNSTGIWIAAG
                     VVFCYAVFGGMKGITYTQVAQYVVLIIAYTIPAVFISLQLTGNPIPMFGMFSEHTESG
                     MPLLQKLNEVVAGLGFTDYTADVTNKLNMVLFTMSLMIGTAGLPHVIIRFFTVPKVAD
                     ARWSAGWTLIFIALLYLTAPAVASMARLNLMTTIYPQGVEAPALAYEERPEWVKTWET
                     TGLIKWEDKNADGRIQLYNDAAPAFTPTAETRGWKGNELTVNNDILVLANPEIANLPG
                     WVIGLIAAGAIAAALSTAAGLLLAISSAVSHDLIKNMINPKISEKGEMLAARISMAIA
                     ILLATWLGLNPPGFAAQVVALAFGIAAATLFPALMMGIFSKRVNDKGAIAGMLVGLVF
                     TVVYIFLYLGWFFIPGTATFANTPDNWMFGISPLSIGTVGALINFAVAYAVSLATVAP
                     PKEIQDLVESVRTPKGAGGALSH"
     gene            complement(9621..>9826)
                     /locus_tag="VFF22_10160"
     CDS             complement(9621..>9826)
                     /locus_tag="VFF22_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HZX17212.1"
                     /translation="TWSLIVWALVSYGFAILLRPMLAGISVGGTDLGFWFAQQGSIIT
                     FIVIIFFYAWKMNRLDKQFGVEE"
BASE COUNT         2056 a         3157 c         2848 g         1765 t
ORIGIN      
        1 gcccggccag ccagatcgga gccgacgacg ccagcctggc cgccaccttc gcccgcctta
       61 ccgcggaggt catatgaggt atctcgtagg gtgcgccgtg cgcaccagcc ttcttcgggc
      121 acgcaccaca ggcaaaaaac actctcaccc tctcagccgt gcacggccta cagatcgcgc
      181 atcagcaggc catattccag ctgcacctcg ggcggcaccg gcaggtacag cacatggccg
      241 tcgccgggcg cgaccgccac ggcctggccc ttgctgtttt ccagctgatc tagggtaaag
      301 cgcagattgc cctggggcgt catcaactca aggccatcgc cgacggcaaa acggttcttc
      361 acccgcacct cggccaactc accacggcgc tcgccggtca gctcaccgac aaactgctgc
      421 tgctcggaca ccgaactgcc gcgctggtag ttctgatatt catcgtgcac atggcggcgc
      481 agaaagccct cggtgtagcc ccgatgagca agtgactcca atgtgtccat caggctcttg
      541 tcgaagggtt tgcccgcgac cgcatcatcg atggccttgc ggtacacctg agcggtgcgc
      601 gccacgtaat agtgggattt ggtacggcct tcgatcttca gcgaatgcac gcccatctgc
      661 accaggcgct cgacgtgctg caccgcacgc agatccttgg agttcatgat gtaggtgccg
      721 tgctcatcct caaatgccgc tatctgctcg ccggggcggc tggcatcttc cagtagaaac
      781 agctgctcgg tgggtgcgcc tacgcccagg gtcggttcga caatctgcac cacttcgccc
      841 agttcgttct cgcccgcctg ttcagccttg tactgccagc ggcaggcatt ggtgcagctg
      901 ccctggttgg ggtcgcgttt gttgatatag cccgacagca ggcagcgtcc ggaataggcc
      961 atacacagcg cgccgtggac aaacacctcc agctccatgc ccggcacttc gctgcgaatc
     1021 tcggcgattt cctccagcga cagctcgcgc gacagaatca cccggctcaa gccctgctgc
     1081 ttccagaact tcacactggc ccagttcacc gcattggcct gcacggagag gtgaatcggc
     1141 atctgcggga aatgttcacg caccagcata atcagcccgg ggtcggacat gatcagcgca
     1201 tccggcccca tcaacacaac gggcaaaagg tcacgcagaa aggtcttcag cttggcgtta
     1261 tgcggcgcga tattgaccac cacatagaac tgcttgccct gggcatgcgc ctcgcgaatg
     1321 cccgcctcca gattgtcgtg gtcgaactcg ttattgcgca cccgcaggct gtaacgcggc
     1381 tgcccggcgt acaccgcatc cgcaccgtaa gcgaaggcgt aacgcatgtt cttcagggtg
     1441 ccggctggcg agagcagctc ggggtaacgc ggagtcgatg ccatgggtca agcgctcagc
     1501 caacagggct ggccactcta gcgcggcagc ggccagcggc tattgatctg catctatgtc
     1561 acggcgcagg caggacgcga cgcaaccacc tatcgcgaca gcgtaacgac cgattgcagc
     1621 gcagggtacg gcgctgtcat catatgcgaa taattctcga tttgtttcct atcttctatt
     1681 gcgaggtctg cgtgtcggcc ggcgattcca cccaccagca acttgtcggc ctgttctatc
     1741 gcgaccaccg cgattggctg ctcggctggc tgcgcaagag cctgtacagc ccggagcgcg
     1801 ccgaagacct cagccaggac actttcgtgc gcctgctcgg ccgcagcgac ctagaccagg
     1861 ctcgcgaacc acgcgcactg ctgaccacca tcgccaaggg tctgctgatc gaccacttcc
     1921 gccgcagcgc gctggaacgc gcttacctcg aagaactcgg acaacagcca cacgacgtgc
     1981 aacccagcgc cgaagaacag gcgctggccc tggacgcact gcgcgagatc gaccgcctac
     2041 ttggccagct gtcgagcaat gcacgagcgg cctttctgca taaccgcctg gatggcatgg
     2101 gccacgcgga aatcgccgag cgcctcggtg tgtcggtatc ccgcgtgcgc cagtacctgg
     2161 cccagggcct gcgccagtgc tacatcgccc tgtatggagc gccgcaatga gcgcggtatc
     2221 ggcacaggtg ctggatgcgg ccatcgaatg gcaactgtgc ttggactccg gcacggtcag
     2281 cgccgaacag cagcacgcct tcgccgaatg gctggcggcg caccccgaac atgcgcgagt
     2341 ctggcagcag ctcaatggcc tggatattcg tctggcttcg gccgccgccg caccggcgcg
     2401 ccaggccttg ctgcacagcg cgcgcagcaa aggactgcgt cgtatgggcg gcaccgcgct
     2461 cggcctgctg ctgagcagcg cgctggcgct cggcctgttg gcgcagcaac gcccgcttgg
     2521 cgactacctg gccgatgagc acaccggccg tggcgaacag cgcgaactgt tgctcagcga
     2581 ccgcagcctg ctgcgcctga acagcgccag tgccgtggat atcgaattca gccgaacaca
     2641 acgtgcagtg cgcctgcgca gcggtgaaat cctcgtgcaa accgctcatg gtgatacgcg
     2701 gccctttgta gtgctgaccg aacatggcag cctgcgcgcc ctcggcaccc gctttctggt
     2761 acgccgtgaa ggcaacagca cacgcctgac ggtgctgcaa tcggccgtag tcgcacaccc
     2821 ggcagaaggc aacagcgaac gcattatcca gagcggtgag caggtgctga tgcagcgtca
     2881 gcgcctgggc agcgtactgc ctgcaccact tagcgcggcg gcgtggagcc aaggcatgct
     2941 gatggccgac aatctgcgcc tggccgattt gctcgacgcg ctgggcgaat accgcagcgg
     3001 ctacttgggc ctcgacccga gcctggccaa cctgcgcatc agcggcagct ttcccctgca
     3061 tgacagcgac aaggccctgg ccgccctgcc gccgagtttg ccggtgcgta tcgaacgctt
     3121 tggcaactgg tggaccagag tcgtacctgc agcaccctaa gcggccgcct aaaaaccaag
     3181 cggacagaca agacagggac tgacgtggga ggggctttag ccgcgacatt gcagtgtcgc
     3241 ggctaaagcc cctcccacaa agagccgtcg cccagttagg ggtcgacaac agcgccttga
     3301 gatagcgcta aaaaataatt tcaaacagcc ctgtcacttt tgctttctgc ttcggcaaca
     3361 ggtcattgag atacattccc gcttaggaga gtttcgtaat gacccgcgcc ctgcccctgt
     3421 tccgccccgg cctactggcc gtcgccatca gcctcgccag cgtctgcgct cccgccctgg
     3481 cggatagcta ccagctgccg gccgcaccgc tggccagcac cctgacgcag atcgccagcc
     3541 aggccggcat cgtcctgagc attgaccccg cgctgactgc caacaaacaa tcagccccgg
     3601 taacaggtga ctacgacgcc ctcggtgccc tgcaacaggc gctgcaaggc agcggcctac
     3661 agttgcagca gaatagcgcc ggcacctaca acctggcccc cgtgccgcaa gcggcggtgg
     3721 cgttgccgga tgttacggtg accagccaag tcgtcaacga aggcagcgca gcgtccggct
     3781 accgcagcga taccgcgcag aatatcggcg ccctcggcgg tatgcgtttg caggacgcgc
     3841 cctactcggt cagcgtggtg tccaaggaac tgctgcagaa catccaggcc acttccagcg
     3901 atgacgtgtt caagctcagt ccggccaccc agttcacttc gcccgtcagc gccggttatg
     3961 ccagcgcggt gagcatgcgc ggttttagcg ggtccggtaa tttgagcatc gccaacgatg
     4021 gcctgcgttt cagcaacagt tatgacggcg gcaatttcat cgaagagatg gagcggctgg
     4081 aaattcttac tggcctcagc ggtttcctct acggcccggc cagccctggt ggcctggtca
     4141 actacgtgct caagcgcccg acctatgagc gctataacag cgtgaccctg ggcaatgccg
     4201 gcggcgagaa ctactacctg cacggcgact tcggcggccc gatcgacgac gccggcgtct
     4261 ttgcctaccg cgtcaacgtg ctgacccagg atggtgaaac cgccctcgac ctgcagaagc
     4321 gccgccgcga aatgatcagc gtggcactgg actggaatgt cagcgacgat ttgcagatcc
     4381 agttcgatgc ctcgcacaag aaaactgaga atcgcggcct gaccagctac tggtatttcg
     4441 ccgaccaggc ccgccgacca gacgccaagg atctcgacac cgacaaactt tacagccaga
     4501 agtgggccta cgccgacagc gaacatgatc gtatcggcag ccgcttcaac tggcgcctgg
     4561 acgaaacctt caccctgcgc ggcgccctgg cctacagcca gtacaccgag gaatacgcct
     4621 acaccggccc gactgtggaa aacgccaccc cgattggcca atacacgcag ccgctatatg
     4681 ccttcgcccc ggtagaaaca gaagaaacct caggcaatct gtttctcgac gccgagttca
     4741 ccaccggcag catccagcac aagaccacac tgggctatca gggcaatgtc gcgagggtgc
     4801 gcaactacac cgatgccggc gtctacggtc gcaacatcgg catcagcgtt ccctttggcg
     4861 aacaaccgca gatcgacaaa cctgactttt ccgccgacat cggccatggc gaccagcgcc
     4921 tcgtttcgcg cacccagtca gacaacctgc tgatcggtga cgtgattaca ttcaacgagc
     4981 agtggtcgag catccttggt ctgacccgca cccgtattga aacctggagc gccgggatca
     5041 ataacgcaga gacggaaaac tctccgaacg tctcgctgat ctacaagcca caaccctggc
     5101 tgaccaccta tgtcagctat atcgagggtt ttcaggccgg cggcctggcc ccctggaatg
     5161 ccgtcaacgt caacgaaacc atggctccgc tggtcagcga gcagtatgaa gtgggcgtca
     5221 aggctaccgt cggcgaaacc ctgctgaccc tggccctgtt caatatcgac aagcccaatg
     5281 cctatatcaa cggtgccggc tactacgtcc aggacggtcg ccaggagaac aacggtctgg
     5341 agtttggtat caccggcaaa gtgctgcccg aactgactct ggtcggtggt gtgaccctgc
     5401 ttgatccgca ggtgaaggac tccggcgctg ccgccagcga agggcagaaa cccaccgacg
     5461 ttgcctccca gctggccaag ctgtatgccg agtacgacat caacgctgta ccgggcttgg
     5521 cgctgaccgg cggtacttac tacaccggca agcagtacag cgacgaagcc aaccagcaca
     5581 gcctgccgtc cttcaccacc ttcgatgccg gcgcgcgtta ccgcgcacag ctgagcagcg
     5641 acaacaccct gaccctacgc accaacatca gcaacctggc gaacaaggaa tactggctaa
     5701 gcagccatta cctgggcgcg ccgcgcaccc tgaccttctc cgctcagctg gaattctgat
     5761 cacaccgcag cacacctgcg ccgccatacc gggcggcgta ggcatttctg gataggccga
     5821 tgaaaagcac caccattcgc cgctggtccc aggtacacac ctggaccagc ctgatctgca
     5881 ccctgtttct gctgatgctc gccctgaccg gcttaccgct gatctttcac catgagctgg
     5941 agcacctgct cggtgaagcc ccagaactac gcgagctgcc ggcgaacacg ccgcacctga
     6001 gcctgcaaca gttggtcgag gctgccgagc gccatcgcca gggcgaggtg atccagtacc
     6061 tgggctatga cgaggatgaa cccaatggcg tggtggcgat taccgccgcc accgccggca
     6121 ccgaaccgaa ctcctcacac acctttatgc tcgatgcccg cagcggcgag gcgattgaga
     6181 tgccggcggc caatggcggc atcctgatgg tcattctgcg tttgcatgtg gatatgttcg
     6241 ccggcctgcc cggcaagctg ttgctggcct ttatgggcct gctgtttgtg gtggcaatcg
     6301 tgtccggcgt ggtgctctat gcgcccttta tgcgtcggct gaaattcgcc gaggtacgcc
     6361 atgagaaagc cactcgaacg cgctggctcg acctacacaa cctgatcggc atcgtcaccc
     6421 tgacctgggc gctggtggtg ggcgtcaccg gggtgatcag cgcctgtgcc gatctgctga
     6481 tcagcgcctg gcgcaccgaa tccctggcag cgatggtcga gccttaccgc gatgcgccgc
     6541 cgctgaccca gcgagcgccg gtgaacgacg tgctgactat cgccgccgag gccacgccgg
     6601 gtatgcagcc ggactttatc gccttccccg gcacgcgctt ttccagcgag caccactaca
     6661 gcgtgtttat gaagggcaat acgcacctca ccgcgcatct gtggacgcca gtgctgatcg
     6721 atgcacgcag cctggacgtc actgccatcg gccagcggcc ctggtatatg gatgcgctgt
     6781 cgatgtcgca gccgctgcac tttggcgact acggcggcat gccgatgaag atcctctggg
     6841 cggtgctgga tgtgctgacc attatcgtgc tcggcagcgg cttgtatctc tggtgggtac
     6901 gtcgacgtac gccaacgaca caacgtgaac gcgcggagac gacggcatga actggcgcca
     6961 atcgaccttc gccctgccgc tgctgatcgg cctgctgagc gccatcggcc tggtcagtgc
     7021 gctactcggt gatggcggct gggatgctgt ggcctggctt ggcctcgggc taccggccgt
     7081 gattggctgc tggccgttgc tgcggcgcag ctaggctgcc cgtaagccct gaactgggcc
     7141 agggctagcc gatgattagc gaggggaact gacgatgctg cgacctgcta ctggcagacc
     7201 tgccggtaga gcgcatcatc tttagccacg gccttcaact gatccagcag cggctttttc
     7261 tctgcgtcca atggcagcag ttcggccgtg gtgcaattga gcagatgggc ctgatggcta
     7321 acgtggtcga tgtgctgctt ttcaaaacgg tacaggttaa aggcgacgat cgacgtgccg
     7381 cccgcatcct ggcgctgcac gcttttgctg tcgagcacgg taaacgccgt ggtcatcggc
     7441 cagtaaaacg tccagggccg ccagaacact tcaccgtttt cgctggctac cagcaccgac
     7501 tccgccggct gacgctgcag ctggtgctcg aaccaggagt actcgtagta aatctggtag
     7561 ctgagcatgc ccaaaccgcc aaacgccgga atgatccact tcggcaggcg gttgcgcgtc
     7621 agtttgcgca gcagcatggc aataccagcc gcccccaacc ccgacaccac gatggcaagt
     7681 attgtccaca gcattgactg actcccgaaa ataaaaactg tctggcgcag cttttaatgt
     7741 tgcgcagcga cggcccgaag gggccatgga tagctggaaa taaaacgggc ctcctaagga
     7801 ggcccgctag taagaccctg atcagacatg accgggggtg attatgctta gtggctcaac
     7861 gcgccaccag cacctttggg agtacgtacg ctttccacca ggtcctgaat ctccttcggt
     7921 ggcgcaacgg tcgcaagcga cacggcgtag gcgacagcga agttgatcag ggcacccaca
     7981 gtaccgatgg acagcggcga gatgccgaac atccagttat ccggggtgtt ggcgaaggtc
     8041 gccgtgccag ggataaagaa ccaacccagg tacaggaaga tgtagacgac ggtgaacacc
     8101 aggccgacca gcatgccggc aatagcgccc ttgtcgttta cccgcttgga gaagatcccc
     8161 atcatcagcg ccgggaacag cgtagccgcg gcgataccga aggccaacgc caccacctgc
     8221 gccgcaaagc cgggcggatt gagccccaac caggtggcca acaggatcgc gatagccatg
     8281 gagatccgcg cagcgagcat ttcccccttc tcgctgatct tgggattgat catgttcttg
     8341 atcaggtcat gactcacggc tgaggaaatc gccagcagca aaccggcagc tgtcgacaac
     8401 gctgcggcaa ttgcacccgc ggcaatcaga ccaataaccc aacccggcag gttggcaatt
     8461 tccgggttag ccagtaccag aatgtcgttg ttaacggtca actcattacc cttccagccg
     8521 cgagtttcag cagtcggggt aaaggctgga gctgcatcgt tgtacagctg gatgcgaccg
     8581 tcagcgttct tgtcttccca cttgatcaag ccggtggttt cccaagtctt gacccactct
     8641 ggacgctctt catacgccag cgctggagcc tcgacaccct gcggatagat ggtggtcatc
     8701 aagttcaaac gtgccatgga cgccacagcc ggagcagtga ggtacagcag agcgataaag
     8761 atcagggtcc agccagccga ccaacgcgcg tcagcgactt tcggcacagt gaagaagcga
     8821 atgattacgt gcggcagacc ggccgtacca atcatcagcg acatggtaaa gagcaccatg
     8881 ttcagcttgt tggtgacatc agcggtgtag tcggtaaagc ccagacccgc tacgacttca
     8941 ttgagcttct gcagcagcgg catacctgac tcggtatgtt cgctgaacat gccgaacatc
     9001 gggatcggat tgcccgtcag ttgcagggag ataaacaccg caggaatggt gtaggcgatg
     9061 atcagtacta cgtactgagc aacctgggtg taggtaatac ccttcatccc gccaaacacc
     9121 gcgtagcaga acactacgcc ggctgcgatc cagataccgg tagagttgct cacttcaagg
     9181 aagcgcgaga acgccacacc agcaccagcc atctgaccaa tcacgtaggt gacgcagatg
     9241 atgatcaggc agaccactgc agccaggcgt gcaccgttgc tgtagaagcg atcaccaatg
     9301 aagtccggca cagtgaactt gccgaatttg cgcaggtacg gcgccagcag catggccagc
     9361 agcacatagc caccggtcca gcccatcagg aaggttgagt tggcataacc accggcggcg
     9421 atcagaccgg ccatggaaat aaacgaagcc gcggacatcc agtcggctgc agttgccatg
     9481 ccgttggtga cggggtgaac accaccgccg gcgacgtaga attccttggt cgagccggca
     9541 cgggcccaga cagcgatgcc gaagtagagc gcgaaggacg cgcccacaaa cagcatattg
     9601 attacaaact ggctcatgga ttattcctcg acaccgaatt gtttatcgag tctgttcatt
     9661 ttccaggcgt agaagaaaat gatcacgata aaggtgatga tcgaaccttg ctgggcgaac
     9721 cagaagccaa ggtcggtacc gccgacagaa atacctgcca gcatcggtcg aagaagtatg
     9781 gcgaagccgt aagacaccag ggcccagaca atcaggctcc aggtaa
//
LOCUS       DASRUM010000155         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1902320, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000155 DASRUM010000000
VERSION     DASRUM010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902320"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1926)
                     /gene="nirB"
                     /locus_tag="VFF22_10165"
     CDS             complement(<1..1926)
                     /gene="nirB"
                     /locus_tag="VFF22_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003407454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HZX17213.1"
                     /translation="MNSTDTSTDQQRLIIIGNGMVGHHCVEQLIERGALAQYRVQVFS
                     EEPLRAYDRVHLSEYFTGRDAESLALSGAELYQTPGVTLHLGVPVLEIDRERREVITA
                     EGSFPYDQLVLASGSYPFVPPIEGAEGDSRLVYRTLADLDTIQAAAKSARRGVVVGGG
                     LLGLEAANALKSLGLEAHVVEFAPRLMPVQLDDHGGLALKAQIEALGVSVHLSRGTQS
                     ITPGSEYRYRMNFAGDEFLETDLIVFSAGIRPQDAIAKQCGLELGPRGGVAIDSQCRT
                     SDPAIYAIGECAAWNGGIFGLVAPGYQMARSVATQLCGEDSEPFMGADMSTKLKLLGV
                     DVGSIGDAHGATAGSRSYRFIDEANASYRRLVVSADGKQAIGAVLVGDNSYYDTLLQY
                     VQNGIKLPADPSCLILPQGEGAPALGADALPDTATICSCHNVSKGAICAAIDGGCGDL
                     AGLKACTKAATGCGGCSALLKQVFEHELAARGVVVDKSLCEHFAYTRQELYGIVRVEG
                     IESFEALLAKHGRGHVGCDICKPAVGSILASCWNRSITDPALVPLQDTNDTFMANMQK
                     NGTYSVVPRIPGGEITPDGLLAIGAVAKKYDLYTKITGGQRIDLFGAQLHELPDIWAE
                     LIAAGFETGHAYGKSLRT"
     gene            2472..>2630
                     /locus_tag="VFF22_10170"
     CDS             2472..>2630
                     /locus_tag="VFF22_10170"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase"
                     /protein_id="HZX17214.1"
                     /translation="MANKTVRSVCPYCGVGCGIVMQVENGKIAKVSGDQQHPSNFGRL
                     CTKGTTCAQ"
BASE COUNT          451 a          843 c          877 g          459 t
ORIGIN      
        1 ggtgcgcagc gacttgccgt aggcgtggcc ggtctcgaag ccggcggcaa tcagctcggc
       61 ccagatatcc ggcagttcgt gcagctgcgc gccgaacagg tcgatgcgct ggccgccggt
      121 gatcttggtg tagaggtcgt atttcttcgc cacagcgccg atggccagca ggccgtccgg
      181 ggtgatttcg ccaccgggaa tacgcggcac caccgagtag gtgccgttct tctgcatgtt
      241 ggccataaag gtgtcgttgg tgtcctgcag tggcaccagc gccgggtcgg tgatcgagcg
      301 gttccagcag gaggcgagga tcgaacccac cgccggcttg cagatgtcgc agccgacatg
      361 accgcggccg tgcttggcca gcagcgcctc gaagctctcg atgccttcga cgcgaacgat
      421 gccgtacagc tcctggcgtg tgtaggcgaa gtgttcacac aggctcttgt ccaccaccac
      481 gccgcgggca gccagctcgt gctcgaacac ctgcttgagc agcgccgagc agccgccgca
      541 accggtggcc gccttggtgc aggccttgag gcccgcaagg tcgccgcagc cgccgtcgat
      601 ggcggcgcag atcgcgccct tgctgacgtt gtggcaggag cagatggtcg cggtgtccgg
      661 caaggcatcc gcgcccagcg ccggggcacc ttcgccttgc ggcaggatca ggcaggacgg
      721 gtcggccggc agtttgatgc cgttctgcac atactgcagc agggtgtcgt agtaactgtt
      781 gtcacccacc agcacggcac cgatggcctg cttgccgtcg gccgatacga ccaagcggcg
      841 gtagctggcg ttggcttcgt cgataaagcg gtagctgcgc gagccggcgg tggcgccgtg
      901 ggcatcgccg atggagccga cgtcgacgcc gagcagcttg agcttggtcg acatgtccgc
      961 gcccataaac ggctcgctgt cttcgccgca cagctgggtg gcgacgctgc gggccatctg
     1021 gtagcccggc gcaaccaggc cgaagatgcc gccgttccac gccgcgcatt cgccgatggc
     1081 gtagatcgcc gggtcgctgg tgcggcactg gctgtcgatg gccacgccgc cgcgcgggcc
     1141 gagttccagg ccgcactgct tggcgatggc gtcctgcggg cgaataccgg cggagaacac
     1201 gatcagatcg gtttccagga actcatcgcc agcaaagttc atccggtagc ggtattcgct
     1261 gcccggggtg atcgactgag tgccgcgaga caggtgcacg ctgacgccca gggcttcgat
     1321 ctgcgccttc agcgccaggc cgccgtggtc gtccagctgc accggcatca gccgtggggc
     1381 gaattccacc acatgggctt ccaggcccag cgacttcaat gcgttggccg cctccaggcc
     1441 gagcaggcca ccaccgacca ctacgccacg gcgcgcgctc ttggcggcgg cctgaatggt
     1501 gtcgaggtcg gccagggtgc ggtagaccag gcgcgaatcg ccttcggcgc cttcaatcgg
     1561 cggtacgaac ggataagagc cgctggccag taccagctgg tcgtagggga agctgccttc
     1621 ggcggtgatc acttcgcggc gctcgcggtc gatctccagc accggcacgc ccaggtgcag
     1681 ggtcacgccc ggggtctggt acagctcggc accgctgagg gccagggatt cggcatcgcg
     1741 gccggtgaaa tactcggaga ggtgcacgcg gtcataggcg cgcagcggct cttcgctgaa
     1801 tacctgcacg cggtactgcg ccagcgcgcc acgctcgatc agttgctcga cgcagtgatg
     1861 gccgaccatg ccgttgccga tgatgatcag ccgttgctgg tctgtggagg tatctgtgct
     1921 gttcatggca tttcccggcc gaagctcatc aggtttttct gcggcaataa aaaaggcgcc
     1981 tgaaaccttg cggtttcagg cgcctttgcc tgttctgtgc ggcgcctgcg agcggcacct
     2041 gaatacacat cacctataaa ggctgacgat cgccattgat cgaatgggcg gcccgaatgt
     2101 ggcgggctcg ctggggctca tgcagcgcct gtgccagttt ccgcgcagcg gcctgcgacc
     2161 cgcattgcag cgggctacag gcgcagttgc aggctcacct gcacggcgcg ataaagaccc
     2221 aaaaagaggc gagcagggca gcctgcgccc cattatgagt cgcgcaaaac ctggccagca
     2281 gccccgcaca cacctcgcca ggccttgcca cacgggcgtt caagccgccc tggcgaaccc
     2341 tggcgcgtgc cttgctatag accaacccag gtaatcaacg ctgattgccc atccacgaca
     2401 aagacgccgt gccactcccg tttcgacgga gggttgcatg gcgttttgcg tttcaggtgc
     2461 aggagagggg tgtggcgaac aaaacggttc ggagtgtctg tccatattgc ggcgtcggct
     2521 gcggcatcgt catgcaagtg gaaaacggca agatcgccaa ggtcagcggt gaccaacagc
     2581 atccgagcaa cttcggccgc ctgtgcacca agggcaccac ctgcgcccag
//
LOCUS       DASRUM010000156         7087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2244284, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000156 DASRUM010000000
VERSION     DASRUM010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2244284"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VFF22_10175"
     CDS             complement(<1..445)
                     /locus_tag="VFF22_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HZX17215.1"
                     /translation="MAFPFELSVDLTTLAILALVAFIAGFIDAIAGGGGLLTIPALLT
                     AGLPPHLALGTNKLSSTFGSAAASYTFYRRKLFDPSQWRNALIGTAVGALLGAALAQW
                     LPSEWLNQLLPAVVFGCGLYLLFGKTPDAPLLAEAPIAKGRQWPQS"
     gene            547..1194
                     /locus_tag="VFF22_10180"
     CDS             547..1194
                     /locus_tag="VFF22_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17216.1"
                     /translation="MQYGGLAWATALLALLAAFIAARILFNRQWFLGWLRGTCGLAFL
                     AMAALIGLLANDLRSYAPIPEGKPLATLGFKADGPLYRVSVLEGGRERTLTMEGDLWQ
                     LDARFLQWKGLAALIGLQPGYRLEKLSGRFLSIEQQSLAQHTRVELARSPYGIDLWRW
                     LRLGQHDLFIFDPQARRVTYLPLADEAVYSINLTPAGLLAEPMNPAAKQALKDWQ"
     gene            complement(1250..2017)
                     /locus_tag="VFF22_10185"
     CDS             complement(1250..2017)
                     /locus_tag="VFF22_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX17217.1"
                     /translation="MSKTQLFDLDGKIAFVSGASRGIGEAIAKLLAQQGAHVIVSSRK
                     IDDCQKVADAIIADGGKATAVACHIGEMEQISNVFAQIKEQFGRLDILVNNAATNPQF
                     CNVLDTDLGAFQKTVDVNIRGYYFMSIEGGKLMKANGGGSIINVASINGVSPGEFQGI
                     YSVTKAAVISMTKVFAKECAQFGIRCNALLPGLTDTKFASALTKNDAILKIALQRIPL
                     KRVADPSEMAGTVLYLASDASSYTTGVALNVDGGFLS"
     gene            complement(2056..3123)
                     /locus_tag="VFF22_10190"
     CDS             complement(2056..3123)
                     /locus_tag="VFF22_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HZX17218.1"
                     /translation="MALTDQSTRIREGEELDAAVIDQYLKAHIPSLTGEPRISQFPGG
                     ASNLTYLLEYPDKEFVLRRPPFGHKAKSAHDMGREYRILNQLNAGFPYCPKAYVHCTD
                     ESVIGAEFYVMERVNGIILRSDMPAELNFSAEQTCELCKSFIDKMVELHNVDYQACGL
                     GDLGKPEGYVQRQIGGWSDRYDKALTPDAPLWEPVKAWLKDKMPADHHKPAIVHNDYR
                     FDNVILNPQNPSQIIGVLDWELTTIGDPLMDLGNTLAYWIEAGDSAPIQLMRRQPSNA
                     PGMLTRQQFADYYAERSGIEIKSIDFYYTYGLFRLAGIVQQIYYRYFHGQTQDKRFAQ
                     FIHMNKLLEQMSLQVINKSQL"
     gene            complement(3407..3718)
                     /locus_tag="VFF22_10195"
     CDS             complement(3407..3718)
                     /locus_tag="VFF22_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017519529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HZX17219.1"
                     /translation="MSVADIVNTMQSKFNAGAAAGLDLVFQFNIEDGENYSLIVKDGT
                     CAVEKGDNANANVTLIMDTETLKGITSGETDGMQAFMSGKLRAEGDMMLAMKLGELFP
                     V"
     gene            complement(3779..4489)
                     /locus_tag="VFF22_10200"
     CDS             complement(3779..4489)
                     /locus_tag="VFF22_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017737251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZX17220.1"
                     /translation="MGSIYLIRHGQASFGADDYDVLSPIGIRQAEVLGAHLAQIGTRI
                     DRSVSGDLRRQQHTANSALQQLHNAGIAIPSLDIDSAFNEFDADAVIRSLLPGMLGEE
                     PEALHILRNGANNRAEFQRLFAKLIGRWIAGEHDTPDLQSWQGFVEQVQGGLTRLLTQ
                     AESKQNIAVFTSGGTITALLHLITGIAPAQAFELNWQIVNTSLSCLKFRGSQVSLASF
                     NSHVHLQLLKAPELITYR"
     gene            4700..5719
                     /gene="sohB"
                     /locus_tag="VFF22_10205"
     CDS             4700..5719
                     /gene="sohB"
                     /locus_tag="VFF22_10205"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017339179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HZX17221.1"
                     /translation="MEFFAEYIGFLAKTVTLVVAILVVLVTIAALRSKGRQSTGQLQV
                     SKLNDFFKQLHQRMEHAVLDKDQLKAASKAEAKAAKQEKKAGIHKPRVYVLDFDGDIK
                     ASATDSLRHEITALLTMATPQDEVVLRLESGGGMVHSYGLASSQLARIRQAGVPLTIC
                     IDKVAASGGYMMACIGEKIISAPFAILGSIGVVAQLPNVHRLLKKHDIDFEVLTAGEY
                     KRTLTVFGENTEKGREKFQEDLETTHELFKGFVARYRPQLNIDQIATGEVWLGLAALE
                     KQLVDELKTSDEYLAERAKSAELFHLHYAEKKTLQERVGLAGSVALDRFVLNWLSRLN
                     QQRFW"
     gene            5739..6728
                     /locus_tag="VFF22_10210"
     CDS             5739..6728
                     /locus_tag="VFF22_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003279804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HZX17222.1"
                     /translation="MSTFSALQARESAAGGFEQVIVQREIDDLPAGELLIRVKYSSLN
                     YKDALSASGNRGVTKSFPHTPGIDAAGVVEASSVAEFSAGDEVIVTGYDLGMNTSGGF
                     AQYIRIPASWALKRPQGLSLREAMVLGTAGLTAALCVDKLEQAGLTADAGPVLVTGAT
                     GGVGSVALALLTSLGYRVAASTGKAEQADYLKALGAQQIVLRSELQDGTDKPMLKEQW
                     AGAVDCVGGDILFNVVKSLRYGASVACCGLTAGVGFKGSVLPFILRGVNLLGVDSVEL
                     PLVVKASMWDRLSLQWKINLDALVSEVTLEQLPAAIAQVLAGKQVGRVLVNLG"
     gene            complement(6786..>7087)
                     /locus_tag="VFF22_10215"
     CDS             complement(6786..>7087)
                     /locus_tag="VFF22_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HZX17223.1"
                     /translation="IADQLDNFQVIALNFPAFTDGRHTSTAYLLRNRYGYKGEVRAIG
                     DVLRDQLFSYLRVGFDAFALREDKDPLDALKAFEEFSEVYQASTDQPLPLFRRRA"
BASE COUNT         1369 a         1902 c         2263 g         1553 t
ORIGIN      
        1 ggctttgcgg ccactgccgg cctttggcaa tcggcgcctc ggcgagcagc ggcgcatccg
       61 gagttttgcc gaacagcagg tacaggccgc agccaaacac cacggcaggt agcagctggt
      121 tcaaccattc cgacggcagc cattgcgcca acgcggcgcc cagcagggcg ccgaccgccg
      181 taccgatcaa tgcattacgc cattggctgg ggtcaaacaa cttgcgccgg tagaaggtgt
      241 aactggcggc ggctgagcca aaggtcgagc tgagcttgtt ggtgcccagg gccaggtgcg
      301 gcggcaggcc ggcagtcagt agcgcgggga tggtcagcaa gccgccgccg ccggcaattg
      361 catcgataaa gccggcaata aacgcgacca gggcgagaat tgccagggtg gtcaggtcaa
      421 cactgagttc aaacgggaaa gccatgaaaa acacgtcctg tgtctaattg tggtgttctc
      481 ttgctgttcg ccagccaggt agctgcgcat aatgccggca atttcaagca taaggaatgc
      541 acggcaatgc aatacggcgg actggcatgg gcaacagcat tgctggcatt actggcagcg
      601 tttattgctg cacgcatcct gttcaatcgg cagtggtttc tcggttggtt gcgcggcacc
      661 tgcggcttgg catttcttgc aatggcggcg ctgattggtc tgttggccaa tgatttgcgc
      721 agctacgcgc cgattcccga aggtaagccg ctggccacac tgggcttcaa ggctgacggc
      781 ccgctgtatc gcgtcagtgt gctggagggc ggtcgtgagc gcacgctgac catggagggc
      841 gatctttggc agctggatgc acgcttcctg cagtggaagg gcctggctgc cttgatcggc
      901 ctgcaaccgg gttatcgtct ggagaagctc tcggggcgtt ttctctccat cgaacagcag
      961 agcctggcgc agcacacccg tgtcgaactg gcgcgcagcc cttacggcat cgatctgtgg
     1021 cgctggctgc gcttgggtca gcatgacctg tttatcttcg atccacaggc gcgccgagtg
     1081 acttacctgc cgctggccga tgaggcggtg tacagcatca acctgacgcc cgctggcttg
     1141 cttgccgagc cgatgaatcc ggcagccaag caggcgctga aggactggca gtaaattcca
     1201 ggcagtaaaa aagggacccg agggtccctt ttttattgga tgggctatct caggagagga
     1261 agccgccatc cacgttcaac gccacgcccg tggtgtagct ggaggcatcg ctggccaggt
     1321 acagcacggt gccggccatt tcgcttgggt ctgctacgcg cttgagcggg atgcgctgta
     1381 acgcgatttt taagatggcg tcgttcttgg tcaacgccga agcgaacttg gtgtcggtca
     1441 gacctggtag cagggcgttg cagcgaatgc cgaactgcgc gcattccttg gcgaaaacct
     1501 tggtcatgct gatcaccgca gccttggtca ccgagtagat gccctggaac tcgcctggcg
     1561 aaacgccgtt gatcgaagcc acgttgatga tgctgccgcc accattggcc ttcatcagct
     1621 tgccgccttc gatggacata aagtagtagc cacggatgtt gacatcgaca gtcttctgaa
     1681 acgcgcccag gtcggtgtcc agtacgttgc agaactgcgg gttggtggct gcgttgttga
     1741 ccaggatgtc gaggcggccg aactgctcct tgatctgtgc gaagacgttg ctgatctgct
     1801 ccatctcacc aatatggcag gccacggcgg tagccttgcc gccgtcggcg atgatcgcgt
     1861 cggcaacttt ctggcaatcg tcgatcttgc ggctggagac gatcacgtga gcgccttgct
     1921 gggccagcag tttggcgatg gcttcaccga tgccacggct ggcgccggag acgaaagcga
     1981 tcttgccgtc gaggtcgaac agttgagtct tggacatggg cttttcctta tctggtgaga
     2041 ggcctgttcg gccgtttaca gctgcgattt attgatgacc tgcaggctca tttgctccag
     2101 cagcttgttc atgtgaatga actgcgcgaa gcgtttgtcc tgggtctggc catggaagta
     2161 gcggtagtag atctgctgca cgatgccggc caggcggaac aggccgtagg tgtaatagaa
     2221 gtcgatgctc ttgatttcga tgcccgagcg ctcggcgtag tagtcggcga actgctggcg
     2281 ggtaagcatg cccggcgcat tgctcggctg gcggcgcatc agctggatcg gtgcggaatc
     2341 accggcttcg atccagtagg cgagggtgtt gcccaggtcc atcaatgggt cgccgatggt
     2401 ggtcagctcc cagtccagta cgccgataat ctggctcggg ttctgcgggt tgaggatcac
     2461 gttatcgaag cggtagtcgt tatgcacgat ggccggcttg tgatggtcgg cgggcatctt
     2521 gtccttgagc caggccttga ccggctccca cagcggcgcg tcgggggtca gggctttgtc
     2581 gtaacgatca ctccagccac cgatctgccg ctgcacataa ccttctggct tgcccagatc
     2641 acccaggccg caggcctgat agtcgacgtt gtgcaactcg accatcttgt cgataaaact
     2701 cttgcacagc tcgcaggtct gctcggcgct gaagttcagc tcggccggca tgtccgaccg
     2761 caggatgatg ccgttgaccc gttccatcac atagaactcg gcaccgatca ccgactcgtc
     2821 ggtgcagtgc acataggctt tcgggcagta ggggaagccg gcgttgagct gattgaggat
     2881 gcggtactcg cggcccatgt cgtgggcgga tttggccttg tggccaaagg gcgggcggcg
     2941 caggacgaat tctttgtccg ggtattccag caggtaggtc aggttcgagg cgccgccggg
     3001 gaattggctg atacgcggct cgcccgtcag actggggatg tgcgccttga ggtactggtc
     3061 gatgaccgcg gcgtcgagtt cttcgccttc gcggatacgg gtggactggt cagtaagcgc
     3121 catgtttatc ccttctgctt atgatgggtg cctaagataa ttagctaatc taatgctgca
     3181 gcctacggct cacaaggatt acccgccgtt ataggtgggc gtgttgctac ccaatcaagc
     3241 ggcctgatcg gccattcaag cttagtttct agccataaaa caatggtcgg gagccagtgt
     3301 taacgtcgcg tagcgacggc ccgtagggtg gccgccatgg atggcggcca taaaaaaacc
     3361 ggagtcgagg actccggttt gttttgtagc gcagtagctt tgccgcttaa accgggaaca
     3421 gctcgcccag tttcatggcc agcatcatgt cgccttcggc gcgcagtttg ccggacataa
     3481 acgcttgcat accgtcggtt tcgccgctgg tgatgccttt cagggtttcg gtgtccatga
     3541 tcagggtcac gttggcgttg gcgttgtcgc ctttttcaac ggcgcaggtg ccgtctttga
     3601 cgatcaacga gtagttttcg ccgtcttcaa tgttgaattg gaacaccagg tccaggcctg
     3661 cagcggcacc ggcgttgaat ttggactgca tggtattgac gatgtctgca acgctcatgg
     3721 tttcttcctt tttctcaggt tttttctagc gcccgtgatg ggtgcagtgg gccgtagctc
     3781 agcgataagt gatgagctcc ggcgccttca acagctgcaa atgcacatgg ctgttgaagg
     3841 aagccaggct cacttggctg ccgcgaaact tcaggcagct tagtgaggtg ttaacaattt
     3901 gccagttcag ctcgaaagcc tgtgcaggcg cgatcccagt aatcaggtgc agcagggcag
     3961 tgatggtgcc gcccgaggtg aatacggcga tgttctgttt gctctcggcc tgcgtcagca
     4021 ggcgggtgag gccgccctgg acctgctcga caaagccttg ccagctttgc aggtccggcg
     4081 tgtcgtgttc gccggcaatc cagcggccaa tcagcttggc gaacaggcgc tgaaattcag
     4141 cacgattgtt cgcaccattg cgcaggatat gcagggcttc aggttcttcg ccaagcatgc
     4201 ccggcagcag gctgcggatc accgcgtcgg catcgaactc attaaaggca gagtcaatgt
     4261 ccagtgacgg tatggcaatg ccggcattgt gcagttgctg taaggcgctg ttagccgtgt
     4321 gctgctgacg acgcagatca ccgctgacac tgcgatcaat acgcgtaccg atttgcgcga
     4381 gatgcgcgcc caacacttcg gcctggcgaa ttccgatggg cgatagcaca tcgtaatcgt
     4441 ctgcaccgaa ggaggcttgg ccatgtcgaa tcaggtagat gctgcccacg tccgctcgat
     4501 tccggtacgt tgaaagtttc gcgagggtat gaggaagctc aggagctgtc aacgtaaaaa
     4561 catacgcttg tttgaattgc atgagtgcgc tgaatagctg tggtctgttt gctggtcgcc
     4621 aatccatggc ctcggtatgc ttgggcacta ttgcgcgcgg tcgatgagtg cgcgctgcca
     4681 ttgggacaag ggggaatacg tggagttttt tgcagagtac atcggctttc tggccaagac
     4741 agtgaccttg gttgtggcca ttctggttgt actggtgacc atcgcggcac tgcgtagcaa
     4801 gggtcgccag agcacgggcc agctgcaggt gagcaagctc aatgacttct tcaagcagct
     4861 tcatcagcgc atggagcacg cagtgctcga taaggaccaa ctgaaagcgg cgagtaaagc
     4921 cgaagccaaa gccgccaagc aggagaagaa ggctggcatc cacaagccgc gcgtctacgt
     4981 gctggatttc gacggtgata tcaaagcctc ggccactgac agccttcgtc acgaaatcac
     5041 tgctttactg actatggcta cgccgcagga tgaagtggtg ctgcgtttgg aaagtggcgg
     5101 tggcatggtg cacagctatg gtttggcttc ttcacagctg gcgcggattc gtcaggccgg
     5161 cgtgccactg accatctgca tcgacaaggt cgcggccagc ggcggctata tgatggcttg
     5221 cattggcgag aaaattatct ccgcaccgtt cgccatcctc ggctcgattg gtgtggtggc
     5281 gcagctgcct aatgtgcacc gcctgctgaa aaagcatgac attgatttcg aggtacttac
     5341 cgccggtgag tacaagcgca ccctgaccgt gttcggcgag aacaccgaga agggccggga
     5401 gaagttccag gaagacctgg agaccaccca cgagttgttt aagggctttg ttgcccgcta
     5461 ccggccgcag ctgaacattg accagatcgc cactggcgaa gtctggttgg gcctggcggc
     5521 attggaaaag caactggttg atgaactgaa gaccagtgac gaatacctgg ctgagcgagc
     5581 gaagagcgcc gagctgtttc atttgcatta tgccgagaag aaaaccctgc aggagcgcgt
     5641 tggactggcg ggcagcgtgg cgttggatcg cttcgttctg aactggctga gtcgtttaaa
     5701 tcagcagcgc ttctggtaac cgttttaagg gagtcgatat gagtacgttc agtgcattgc
     5761 aggcccgtga aagcgcagcg ggcggtttcg aacaagtgat tgtgcaacgt gagatcgatg
     5821 acttgccggc cggcgagttg ctgattcggg tcaagtattc ctcgctcaac tacaaggatg
     5881 ctttgtcggc cagtggcaac cgcggtgtga ccaagagctt tccgcacacc ccaggcatcg
     5941 atgctgccgg tgtggttgaa gcctccagcg tggccgagtt cagcgcaggc gatgaggtga
     6001 ttgttaccgg ctacgacctg ggcatgaaca cctcaggcgg ctttgcccag tacattcgca
     6061 ttcccgccag ctgggcgctg aaacgccccc agggcttgtc actgcgcgag gctatggtgc
     6121 tcggcactgc tgggctgacg gcagcgttgt gtgtcgacaa gctggagcag gcagggctca
     6181 cggcggatgc cggccccgta ttggtaactg gggccaccgg cggcgtcggc agtgtcgcac
     6241 tggccttgct cacttctctg ggttaccgcg tggcggcgtc taccggcaag gccgagcagg
     6301 cggattacct caaggcgcta ggcgcgcagc aaattgtgct gcgttccgaa ctgcaggacg
     6361 gcaccgataa gccgatgctc aaagaacagt gggctggcgc ggtggattgt gtgggcgggg
     6421 atatcctgtt caacgtggtc aagtcgctgc gctacggtgc cagtgttgcc tgctgcggcc
     6481 tgacggctgg cgtcggcttc aagggcagtg tgctgccgtt tatcctgcgt ggggtgaatc
     6541 tacttggggt cgattcggtc gagctgccac tggtggtcaa ggcctcgatg tgggaccggt
     6601 tgtcgttaca gtggaagatc aatctggatg cgctggtcag cgaagtgacg cttgagcaat
     6661 tacccgcggc tattgcccag gtgcttgcag gcaagcaggt cgggcgagtg ctggtcaatc
     6721 tgggctgagc cgcgagaaac aaaaaaggcc accccttggg gtggcctttt gcgttaccgg
     6781 ctggatcagg cgcggcggcg gaacagcggc agcggctggt ccgtggaggc ctgatagacc
     6841 tcactgaact cctcgaaggc tttcaaggca tccagcggat ctttgtcctc acgcagggcg
     6901 aaggcatcga agcccacgcg caggtatgag aacagttgat cgcgcagcac atcaccaatt
     6961 gcacgcactt cgcctttgta gccgtagcgg ttacgcagca ggtaggcggt ggaggtgtgg
     7021 cgtccatcag taaaggccgg gaagttcagc gcgatgacct ggaagttatc cagctgatcg
     7081 gcgattt
//
LOCUS       DASRUM010000157        15817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1593711, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000157 DASRUM010000000
VERSION     DASRUM010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 15817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15817
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593711"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /gene="prfC"
                     /locus_tag="VFF22_10220"
     CDS             <1..489
                     /gene="prfC"
                     /locus_tag="VFF22_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252592.1"
                     /note="stimulates the release of release factors 1 and 2
                     from the ribosome after hydrolysis of the ester bond in
                     peptidyl-tRNA has occurred; GDP/GTP-binding protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HZX17224.1"
                     /translation="NHGTIQIGDTFTEGEALGFTGIPHFAPELFRRVRLKDPLKSKQL
                     RQGLQQLAEEGATQVFFPERSNDIILGAVGVLQFDVVASRLKEEYKVECAYEPITVWS
                     ARWIDCSDKKKLEEFSNKAVENLALDGGGHLTYLAPTRVNLSLMEERWPDVKFRATRE
                     HH"
     gene            complement(595..993)
                     /locus_tag="VFF22_10225"
     CDS             complement(595..993)
                     /locus_tag="VFF22_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HZX17225.1"
                     /translation="MPKGTQRPTAEQREFAQRLRHQFTDCEQLLWQHLRNRQLGGFKF
                     RRQYPLPPYVLDFYCAELGLTVELDGGQHFGDEALRRDTERSRYLEACGVRVIRFSNR
                     EVLAQMPEVLAEILRQAEIAAPHPNPLPRG"
     gene            complement(1265..3004)
                     /locus_tag="VFF22_10230"
     CDS             complement(1265..3004)
                     /locus_tag="VFF22_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HZX17226.1"
                     /translation="MNLLIVTACPNGQVTSVLCSRLLDAAAQRLGWSTRVEVHDPKAI
                     GSPLSAADIAAAELVVVVKTGALDLQRFVGKRVVQSTPSQALIDPQQFLRDAAEQAEV
                     LDAQAIQAAVPAAQAPRLVAITACPTGVAHTFMAAEALQQAAAQMGYVLHVETRGSVG
                     ARNLLEDADIAAADAVLLATDIEVDTSRFAGKKVYRCGTGVALKQPRETLDRALAEAQ
                     PLQGGAGNDAGQSAAGSSQAGPYKHLLTGVSFMLPMVVAGGLLIALSFVFGIEAFKEE
                     GTLAAALMQIGGDAAFKLMVPVLAGYIAYSIADRPGLAPGMIGGLLASSLGAGFIGGI
                     VAGFLAGYAAKAIARWVQLPASVEALKPILIIPLLASLFTGLVMIYVVGKPVAGMLAG
                     LTTFLDSMGSTNAILLGLLLGAMMCVDLGGPINKAAYAFSVGLLASQSYAPMAATMAA
                     GMVPPIGMAIATLLARRKFAQSEREAGKAAGVLGLCFISEGAIPFAAKDPLRVIPASI
                     AGGALTGALSMYFGCKLMAPHGGLFVLLIPNAINHALLYLLAIVAGSLLTGVVYAVIK
                     KGAAVELVVGAKT"
     gene            complement(3019..3957)
                     /gene="pfkB"
                     /locus_tag="VFF22_10235"
     CDS             complement(3019..3957)
                     /gene="pfkB"
                     /locus_tag="VFF22_10235"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HZX17227.1"
                     /translation="MASILTLTLNPALDITVSLDTLTLGSVNRSLGLIQHAAGKGLNV
                     AQVLTDLGHSLTVSGFLGADNAAPFEALFAARGLHDAFIRVPGETRSNIKLSEHDGRV
                     TDLNGPGVEVSEAHQAALLAQLDALVVGQDAVVVAGSLPRGVSVGWFAELLGRLNALG
                     VPVALDTSGEALRTGLAATPWLVKPNDEELAEACDLHDASLAALSEAAERLRAQGVAH
                     VLLSRGAAGVSWFGPGVALQAQPPQVQVVSTVGAGDSLLAATLHGLLNGWPAEQTLSL
                     ATAVAAQAVTQLGFGIQDREQLAHLQAGVTVMPLEQ"
     gene            complement(3957..6821)
                     /gene="ptsP"
                     /locus_tag="VFF22_10240"
     CDS             complement(3957..6821)
                     /gene="ptsP"
                     /locus_tag="VFF22_10240"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HZX17228.1"
                     /translation="MLELNAAQIQVGHSAPDKQAALALLAEALSADGLVAPGYLAGMQ
                     AREVQGSTYLGHGIAIPHGTPQTRDLVFHTGVRLIQFPQGVDWGDGQRVYLAIAIAAR
                     SDEHLRLLQLLTRALGEGDLSQALREATAAEQILELLQGAPQALALDSQLIGLGVQAE
                     DFDELIWQGARLLKKAGCADSGFANSLAQGTPLPLGNGLWWLNSEEAVSRPGLAFVTP
                     AASLRHGGQVVSGLFCLASAGEAHRQLLERLCDLLINGQGGTFSHPTSSRSVLEALGG
                     EVAPDWPSLRIVLANTHGLHARPAKELSEIAQAFDGDIRVRLDEQAAGVSAKSLSKLL
                     ALGAQRGQTLIFSAEPSIAGDALPALEAAVREGLGETVEPLPVTSSVPAAAAVARVTP
                     LPAPQAGARLQAVAASPGIAIGPALLRLVPQLEYAQQGQGSEQERTRLDEALAQIEAD
                     IQHLIEASDEASVRDIFVTHQAMLRDPALREDVDARLAQNLSAEAAWNAEIEAAAQQQ
                     EALHNVLLAERAADLRDIGRRVLARLCGVEAPREPDEPYILVMDEVAPSDVASLNRQR
                     VAGILTARGGATAHSAIIARALGIPAVVGAGEGVLALAQGTVLLLDGDRGLVRVAPDP
                     QTLEQAQREREANQARRERAHAERFEPAVTRDGHSVEVFANIGASGETVQAVDLGAEG
                     IGLLRTELVFMDHAQAPDLATQEAEYRRVLDALNGRPLVVRTLDVGGDKPLPYWPMPA
                     EENPFLGVRGIRLSLQAPQLLETQLRALFASADGRALRIMFPMVGQVEEWRVARDMAL
                     RLREEIPVAELQLGIMIEVPSAALLAPVLAREVDFFSVGTNDLTQYTLAIDRGHPTLS
                     AQADGLHPAVLRLISMTVEAAHAHGKWVGVCGELAGDMLAVPLLVGLGVDELSVSARS
                     IALVKARVRELDLSHSQTLARDALLLDSASAVRALVEGQR"
     gene            6986..8002
                     /gene="cra"
                     /locus_tag="VFF22_10245"
     CDS             6986..8002
                     /gene="cra"
                     /locus_tag="VFF22_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HZX17229.1"
                     /translation="MKLSDIARLAEVSVTTASYVINGQANKRRISAATVARVLEVVER
                     HGYRPDQQAAGLRRGQSRCLGFILPDLENPSYARLAKLLEQRARSAGYQLLIASSDDD
                     PECERQLLELFRSRRCDALIVASCLPQDDPIYPRLVTSGVPVIAVDRALDPTQIRSVV
                     SDDQQAGSLLTTTLLSPPPRHIALIGARGELPISQARERGFHQALDGFAGRISVCHAE
                     QFSRASGCQQMHALLADPAGVPDALITTAYVLLEGVLDALQAHNAEDFTELRLATFGD
                     TQLLDFLPLRVNAISQQHEQIAERVLALAIAAIEQNHYQPGIEAIPRLLKIRRTLKEK
                     PGGV"
     gene            8010..8789
                     /locus_tag="VFF22_10250"
     CDS             8010..8789
                     /locus_tag="VFF22_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZX17230.1"
                     /translation="MHLIDTHTHLDFPDFDADRSELLTRSRKLGVERLVVLGVYQANW
                     QRLWDLVQGDAGLYAAFGLHPVYLDDHKPAHLSELRDWLQRLAGHPQLCAVGEFGLDY
                     FLEQLDRAQQQQLFEAQLKLAIEFELPALLHVRRAHAATIATLKRLKPARGGIIHAFA
                     GSTEEAREYLKLGFKLGLGGAPTWPQALRLRKVVAQLPLDAIVLETDSPDMAPAMHPN
                     QRNSPEHLPDICQALAELRGISADELARASSHNAAELFGWN"
     gene            complement(8790..9827)
                     /locus_tag="VFF22_10255"
     CDS             complement(8790..9827)
                     /locus_tag="VFF22_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZX17231.1"
                     /translation="MPFANDLIEHNCERLAEQSRSATLLSRFVFEPAQVMALLRARIV
                     GQDAVLAEVDALLKVLKVDIGERERPLTVNLFMGPTGVGKTEIVRLLAQAIHGRPDAF
                     CRIDMHTLAQEHYAAALTGAPPGYVGSKEGTTLFDAEAIQGSYSRPGIVLFDELEKAS
                     KDVLRSLLGILEHGQLTLAAGTRTLDFRNSMVFMTSNIGAREVSDYRARYARGWRRWL
                     GLSPQADNQVVERALHRRFEPEFLNRIDRILGFQHIEEEWLEALLEIELSKLNQRLLK
                     QGRVLLLDESALDFLCREHDARFGARAMGRRLRTELEPAIADCLLADPSITRLLARVE
                     GGQLRVGADVL"
     gene            complement(9887..10927)
                     /locus_tag="VFF22_10260"
     CDS             complement(9887..10927)
                     /locus_tag="VFF22_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic amidase"
                     /protein_id="HZX17232.1"
                     /translation="MRHGDISSSPDTVGVAVVNYKMPRLHSKAEVLDNARKIAEMIKG
                     MKLGLPGMDLVVFPEYSTMGIMYDKDEMMATAATIPGDETAIFSAACREAKTWGIFSL
                     TGERHEEHPHKAPYNTLILINDQGEIVQKYRKCIPWCPIEGWYPGDRTYVSEGPKGMK
                     ISLIICDDGNYPEIWRDCAMKGAELIVRCQGYMYPAKEQQVLMSKTMAWANNCYVAVA
                     NAAGFDGVYSYFGHSALIGFDGRTLGECGEEEMGIQYAQLSISQIRDARANDQSQNHL
                     FKLLHRGYTGVYNSGDGDKGVADCPFEFYRTWVLDAQKAQEDVEKLTRKTIGVADCPV
                     GELPHPGKERSA"
     gene            complement(11057..11404)
                     /locus_tag="VFF22_10265"
     CDS             complement(11057..11404)
                     /locus_tag="VFF22_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011104861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZX17233.1"
                     /translation="MPIYEYDCPDCGDFTQLRPMAERDAPCTCPYCATPSARVILSAP
                     SLATMSGSQRRAIAANERSANAPQTVEEYAQSRKHPKGCGCCTPNKPLAPTKANPHAL
                     KTKPSARPWMISH"
     gene            complement(11468..12697)
                     /gene="fmdA"
                     /locus_tag="VFF22_10270"
     CDS             complement(11468..12697)
                     /gene="fmdA"
                     /locus_tag="VFF22_10270"
                     /EC_number="3.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004328 - formamidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formamidase"
                     /protein_id="HZX17234.1"
                     /translation="MTETLIKVDLNQPATENEQIHNRWHPDIPMACWVKPGDDFILET
                     YDWTAGAIKNNDDASDVRDVDLSTVHYLSGPVGVHGAEPGDLLVVDLLDIGAMPDSQW
                     GFNGFFSRQNGGGFLTDHFPMAQKAIWDFKGMFTSSRHIPGVNFAGLIHPGLIGCLPD
                     HKMLADWNKREQELIDTDPHRVPALANPPMAPTAHMGKMKGEARDLAALTGARTVPPR
                     EHGGNCDIKDLSRGSKIFFPVYVNGAGLSVGDLHFSQGDGEITFCGAIEMAGWVHMKV
                     ELIKGGMAKYGIKNPVFKPSPITPNYKNYLIFEGISVDEAGKQHYLDVNIAYKQACLN
                     AINYLTKFGYSPAQGYALLGSAPVQGHISGIVDVPNACATLWLPTEIFEFDINPNANG
                     PTKFLDGSIDLPIAPDK"
     gene            complement(12921..13610)
                     /gene="urtE"
                     /locus_tag="VFF22_10275"
     CDS             complement(12921..13610)
                     /gene="urtE"
                     /locus_tag="VFF22_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020294146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HZX17235.1"
                     /translation="MFQISNLASGYGQSQILHDLNLNVAKREIVAVMGRNGMGKTTLF
                     KSLMGVLPQWGGQISVDGCDISKLETHQRVESGIAYVPQGRMIFPSMTVLENIQTGLP
                     ASAKGKVPDDLYALFPVLQEMRHRKGGNLSGGQQQQLAIARALATNPKVLLLDEPTEG
                     IQPSIIKDIARTLKEIRNLRDLTIVVSEQVLSFTMEIADRFLVIEKGKFVVEESRDKV
                     DEATISRYLSV"
     gene            complement(13754..14515)
                     /gene="urtD"
                     /locus_tag="VFF22_10280"
     CDS             complement(13754..14515)
                     /gene="urtD"
                     /locus_tag="VFF22_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011104862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HZX17236.1"
                     /translation="MSQPSAFQMPKPVLAIEGLTVSFDGFKAVDDLNLYIDRNEVRVV
                     IGPNGAGKTTVLDLICGKTKATAGSIQFENRELTKMREFDIVRAGVGRKFQNPSIYEN
                     LTVFENLEMSYPKGRKVFGALFFKRSAEVIARVEQIAAEIMLTDNLYLQADLLSHGQK
                     QWLEIGMLLMQDPALLMLDEPVAGMSVNERMQTAELLKRISKGRSVLVIEHDMEFVKS
                     IAHKVTVLHQGKVLAEGSMEAVQSNPKVIEVYLGH"
     gene            complement(14512..15690)
                     /gene="urtC"
                     /locus_tag="VFF22_10285"
     CDS             complement(14512..15690)
                     /gene="urtC"
                     /locus_tag="VFF22_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HZX17237.1"
                     /translation="MNPTLDKFLGGKQGAIGLAVLATLILVVFPLALDAFRLNMVGKY
                     LTYAFVAVGLVLCWGYGGILSLGQGVFFGLGGYCMAMFLKLEASDPESTKIQSTPGIP
                     DFMDWNQITELPMLWQPFHSFGFTLLAVIAVPVILALVIGVAMFKRRVGDVYFSIVTQ
                     AIALILTVLIIGQQGLTGGVNGITDLKTLMGWDIRDDSSKMILYFINAFLLFGCILIG
                     RFILASKLGRLLMAMRDKEERVRFSGYDVASFKIFVFCVAAAFSAIGGAMFALQVGFM
                     SPSFVGIVPSIEMVIFAAVGGRMSLLGAVYGALLVNYGKTYFSETFPELWLYLMGGLF
                     IAVVMYFPNGLAGVWESHGRKRLEKLLQRFERKSVAPVAAVSPTPAPAPEAKQLEATP
                     "
     gene            complement(15702..>15817)
                     /locus_tag="VFF22_10290"
     CDS             complement(15702..>15817)
                     /locus_tag="VFF22_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010175252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HZX17238.1"
                     /translation="LMEFFMSGSMAKVFTLSAVILILMLRPQGLFSIKVRK"
BASE COUNT         2972 a         5186 c         4897 g         2762 t
ORIGIN      
        1 aaccacggca ccatccagat cggcgacacc ttcaccgaag gcgaagcgct gggctttacc
       61 ggtatcccgc acttcgcccc ggaactgttc cgccgcgtgc gcctgaagga cccgctgaaa
      121 tccaagcaac tgcgccaggg cctgcagcag ctggccgaag aaggcgcgac ccaagtgttc
      181 ttccccgagc gcagcaacga catcatcctc ggcgcagtcg gtgtgctgca gttcgacgtg
      241 gtcgccagcc gcctgaaaga ggaatacaag gtggaatgcg cctacgagcc gatcaccgtg
      301 tggtcggcgc gctggatcga ctgcagcgat aagaaaaagc tagaagagtt cagcaacaag
      361 gccgtggaaa acctcgctct ggatggcggt ggccacctca cctacctggc gccaacacgg
      421 gtcaacctga gcctgatgga agaacgctgg ccggatgtga agttcagggc gacgcgcgag
      481 catcattgat ctttgatcgc aggtaaaagc caaccctctc cctaaatagg agagggggcc
      541 gctcagcggt gacggaaaga tcgcggttag caggctctac gcggccagct ccctctaccc
      601 tctggggaga gggttggggt gaggggcagc gatctcggcc tgtcgcaaaa tctccgccag
      661 cacctcaggc atttgcgcca aaacctcccg attgctaaag cgaatcaccc gtaccccaca
      721 cgcctccaga tagcgactgc gctcagtatc ccgacgcaaa gcctcatcac caaagtgctg
      781 cccaccatcc agctcgactg tcaggcccag ctctgcgcag tagaaatcca gcacataagg
      841 cggcaacgga tattgccgac gaaatttgaa accaccgagc tggcgattgc gcaaatgctg
      901 ccagagcaac tgctcgcagt cggtaaactg atgacgcaat cgctgggcaa attcgcgttg
      961 ctcggccgta ggccgttgag tgcctttggg catgcttcac ctccgtgtga acttcgcccc
     1021 tcaccctagc cctctcccca agggggagag ggaacttacc tgtacgcgcc cagtagatct
     1081 tagcccgtcg cacctaatgc aacgattaaa gctcggtcgg tcagtagcga ctggatgatt
     1141 gtggcagcga gcgctacgcc tatcagctcc ctcgccccta cgggggggga gagggctggg
     1201 gtgaggggca gctatcccag tctgtcgcaa aatctccacc agtacagagc gatcatttac
     1261 ccctctacgt cttcgccccc accaccaact ccaccgccgc gccttttttg atcacggcat
     1321 acaccacccc ggtcagcagg ctgcccgcca ctatcgccag caaatacagc aacgcgtggt
     1381 tgatcgcgtt agggatcagc agcacgaaca gaccgccgtg gggcgccatc agtttgcagc
     1441 cgaagtacat cgacagcgcg ccagtcaggg cgccgccggc gatgctcgcc gggatcaccc
     1501 gcagcgggtc tttggcggca aacgggattg ccccttcgga gataaagcac aagcccagta
     1561 ccccggcggc cttgccggcc tcgcgctcgc tctgggcgaa cttgcgccgc gccagcaggg
     1621 tggcgatggc catgccgatg ggcggcacca tgccggcggc catggttgcg gccatgggtg
     1681 cgtagctttg cgaagccagc aggccgaccg agaaggcata ggcggccttg ttgatcggcc
     1741 cgccgaggtc gacgcacatc atcgccccca gcagcaggcc gagcaggatg gcgttggtgc
     1801 tgcccatgct gtcgaggaag gtcgtcagcc cggcgagcat gccggccacc ggcttgccga
     1861 ccacgtagat catcaccagg ccggtaaaca ggctggccag cagcggaatg atcagaatcg
     1921 gtttgagtgc ttcgacgctt gccggcagtt gcacccaacg ggcaatcgcc ttggccgcat
     1981 agccggcgag aaagcccgcg actatgccgc cgataaaacc ggcccccagg ctgctggcca
     2041 gtagcccgcc aatcatcccc ggcgccaggc ccgggcggtc agcaatggag taggcgatat
     2101 aaccggccag caccggcacc atcagcttga acgcagcatc accgccgatc tgcatcagcg
     2161 ccgcggccag ggtgccctcc tccttgaacg cttcgatgcc aaaaacgaac gacagggcaa
     2221 tcagcagccc gccggccacc accatcggca gcataaaaga cacgccggtc agcaggtgtt
     2281 tgtacggccc ggcttggctg ctgccagcag ccgactggcc tgcgtcgttg cccgcgccgc
     2341 cctgcagcgg ttgggcttcg gccagggcgc ggtcaagggt ctcgcgcggt tgtttgaggg
     2401 ccacgccggt gccgcagcga tacaccttct tgccggcgaa gcggctggtg tcgacctcga
     2461 tatccgtggc cagcagcacg gcgtcggccg cggcaatgtc ggcgtcttcc agcaggttgc
     2521 gcgcgcccac cgagccgcgg gtttccacat gcagcacata acccatctgc gccgcagcct
     2581 gttgcagcgc ctcggcggcc ataaaggtgt gcgccacccc tgtcgggcag gcggtgatcg
     2641 ccaccaggcg tggcgcctgg gcggcgggca cggcagcctg gatggcctgg gcatcaagca
     2701 cctcagcctg ctcggcggca tcgcgcaaga actgctgcgg gtcaatcagc gcctgggacg
     2761 gggtggactg caccacccgc ttgccaacga agcgctgcag gtccagggcg ccggtcttga
     2821 ccaccaccac caactcggcg gcggcgatgt cggcggcact caggggcgag ccgatggcct
     2881 tggggtcgtg tacttcaacg cgcgtactcc agcccaggcg ttgggccgca gcatccagca
     2941 ggcgcgagca gagcacgctg gtgacctggc cattggggca ggcggtgacg atcagcaaat
     3001 tcatagaagg atggcctctt attgttctag cggcatgacg gtcacgccag cctgcaaatg
     3061 cgccaattgc tcgcggtcct gaataccaaa acccagctgg gtgacggcct gggccgccac
     3121 cgcggtggcc aggctcaggg tctgttcggc gggccagccg ttgagcagac cgtgcagggt
     3181 cgccgccagc agcgaatccc ccgcgcccac ggtgctgacc acctgcacct gcggcggctg
     3241 cgcctgcagc gccacgcctg ggccaaacca gctcacgccc gccgcgccgc gcgacagcag
     3301 cacatgggcc acgccctgag cgcgcaggcg ctcggccgcc tcgctcaagg ccgccagcga
     3361 ggcatcatgc aggtcgcagg cctcggccag ttcctcgtca ttgggtttga ccagccaagg
     3421 cgtcgccgcc aggccggtgc gcagcgcctc gccgctggtg tccagcgcca ccggcacgcc
     3481 cagcgcattg agccggccga gcagctcggc aaaccagccg accgacaccc cgcgcggcag
     3541 gctgccggcc accaccacag cgtcttgccc gaccaccaat gcgtcgagct gagccagcag
     3601 cgcggcctga tgcgcttcac tgacctcgac accggggccg ttgaggtcgg tgacccgacc
     3661 gtcgtgctcg ctcagtttga tattgctgcg cgtctcgccg ggcacgcgga taaaggcatc
     3721 gtgcaggcca cgggcggcaa acagcgcctc gaagggcgcc gcgttgtccg cccccagaaa
     3781 gccgctgacg gtcaggctgt ggcccaggtc cgtgagcacc tgggcgacgt tcaggccttt
     3841 gccggcggcg tgctggatca gcccaaggct gcggttcacc gaaccgaggg ttagggtgtc
     3901 gaggctgacg gtgatgtcca gcgccgggtt cagggtcagg gtcaggatgc tcgccatcag
     3961 cgttgcccct cgaccaaggc gcgcacggcg ctggcgctgt cgagcaacag agcgtcacgg
     4021 gccagggtct ggctgtggct caggtcaagt tcgcggaccc gcgccttgac cagcgcgata
     4081 gagcgcgccg agacgctcag ctcgtccacc cccaggccga ccagcagcgg tacggcgagc
     4141 atatcgccgg ccaactcacc gcacacgccg acccacttgc cgtgggcatg ggcggcttca
     4201 acggtcatgc tgatcaagcg caataccgcc ggatgcaggc catcagcctg ggccgagagc
     4261 gtcgggtgac cacggtcgat ggccagggtg tactgggtca ggtcgttggt gccgacactg
     4321 aagaagtcca cctcgcgggc cagcaccggc gccaatagag cagcggacgg cacctcgatc
     4381 atgatgccca gttgcagttc agccaccggg atttcctcac gcagccgcag ggccatgtcg
     4441 cgggccacgc gccactcctc cacctgcccg accatcggga acatgatgcg cagggcgcgc
     4501 ccgtcggcac tggcgaacag cgcgcgcagc tgggtttcca gcaattgcgg cgcctgcagg
     4561 ctgaggcgaa tgccgcgcac accgagaaag gggttttctt cggcaggcat cggccaatac
     4621 ggcagcggct tgtcgccacc cacatccagg gtgcgcacca ccagcggccg gccattaagg
     4681 gcatcgagca cgcggcggta ctcggcctcc tgggtggcca gatcaggtgc ctgggcatga
     4741 tccataaaca ccagttcggt gcgcagcagg ccaatgccct cagcgcccaa gtccaccgcc
     4801 tggacggtct cgccactggc gccgatattg gcgaacactt caacgctatg gccgtcgcgg
     4861 gtcacggcgg gctcaaagcg ctcggcatgg gcacgctcgc ggcgcgcctg gttagcttcg
     4921 cgctcacgct gggcctgctc cagagtctgg gggtcggggg cgacccgtac caggccacgg
     4981 tcgccgtcga gcagcagcac ggtgccttgg gccagggcca gcacgccttc accggcaccg
     5041 accaccgccg gaatacccag ggcgcgagcg atgattgcac tgtgcgcagt cgcaccgccg
     5101 cgtgcggtga ggatgccggc cacgcgctgg cgattgaggc tggcaacatc cgagggtgcc
     5161 acctcgtcca tcaccagaat gtagggttcg tccggctcac ggggcgcctc gacgccacac
     5221 aaccgcgcca gcacacggcg gccaatatcg cgcaaatctg cggcacgctc ggccaacaag
     5281 acattgtgca gggcttcctg ctgctgcgcg gcggcttcga tttcagcgtt ccaggccgct
     5341 tcggcgctta ggttctgggc caagcgcgcg tcaacatcct cgcgcagggc tgggtcgcgc
     5401 agcatggcct gatgggtgac gaaaatatcg cgcacgctgg cctcgtcgct ggcttcgatc
     5461 aggtgctgaa tatcggcctc aatctgcgcc aacgcttcat ctaggcgggt gcgttcctgt
     5521 tcgctgccct ggccttgctg cgcatattcc aattgcggca ccagacgcag caacgcagga
     5581 ccgatggcaa ttcccggtga ggcggccaca gcttgcaggc gtgcacccgc ttgcggcgca
     5641 ggcaacggcg tgacccgtgc tacggctgcg gcagcaggca cgctgctggt gaccggtaag
     5701 ggctcaaccg tttcgccaag gccttcgcgc accgccgctt cgagggccgg cagagcatcg
     5761 ccggcaatgc tgggttcggc gctgaagatc agggtctgcc cgcgttgcgc accgagcgcc
     5821 agcaacttgc tcaggctctt ggccgaaacg ccagcggcct gctcatcaag gcgcacgcga
     5881 atgtcaccat caaaggcctg ggcaatctcg ctcagctcct tggccgggcg cgcatgcagg
     5941 ccgtgggtgt tggccaggac gatgcgcagc gacggccaat ccggcgccac ctcgccgccc
     6001 agggcttcaa gcacgctgcg gctcgaagtt ggatggctga aggtgccgcc ctggccattg
     6061 atcagcagat cacacaggcg ctcaagcaac tgccgatgcg cctcgcccgc actggccagg
     6121 cagaacagac cgctcaccac ctgcccgcca tgacgcaagg acgcagccgg ggtgacgaac
     6181 gccaagccgg ggcggctgac cgcctcttca ctgttgagcc accacaggcc attacccaga
     6241 ggcagcggcg tgccctgggc caggctattg gcaaagcctg agtcagcaca gccggccttt
     6301 ttcagcaggc gcgcgccttg ccagatcaat tcgtcgaagt cttccgcctg cacgcctagg
     6361 ccaatcaatt ggctgtcgag cgccagtgcc tggggcgcgc cctgcaacag ctccaggatc
     6421 tgctcggcag cagtggcctc gcgtagggcc tggctcaggt cgccctcgcc gagggcgcgg
     6481 gtcagcagtt gcaacaggcg aaggtgctcg tccgagcgcg cggcgatggc aatcgccagg
     6541 tacacccgct ggccatcgcc ccagtccacg ccttgaggaa actggatcag ccgcacgcca
     6601 gtgtgaaaca ccagatcgcg ggtttgcggc gtgccatggg ggatcgcaat gccatggccc
     6661 aggtaggtcg agccctgcac ctcgcgggcc tgcataccgg ccaggtaacc gggggcgacc
     6721 agcccatcgg cgctcagggc ctcggccagc aacgccagcg ccgcctgttt gtccggagcg
     6781 ctatggccca cctgaatttg cgcggcattc agttcgagca ttttattgtt ctcctgggca
     6841 ggtgaggctg gctgtgcagg cgcgctgcgc tacccacagg atagccagac gccccgcctg
     6901 ctgaatcgtt tcatcaaatg gatggcacgt taccccataa tcggcgatcc ttgaagcccg
     6961 gctgtcactc tcccaggagc atgccttgaa actcagcgat attgcccgcc tggccgaggt
     7021 ctcggtcacc accgccagct atgtgatcaa cggtcaggcc aacaagcggc ggatcagcgc
     7081 cgccaccgtg gcgcgggtgc tggaggtggt cgagcgtcac ggctatcgcc ccgaccagca
     7141 ggccgccggg ctacgccgcg ggcaaagccg ctgcctgggt tttatcctgc cggacctgga
     7201 aaaccccagc tacgcgcgcc tggccaagct gctcgaacaa cgcgcccgca gcgccggtta
     7261 ccagttgctg atcgccagct ccgacgacga cccggagtgc gaacgccagt tgctcgaact
     7321 gttccgctcg cgccgctgcg atgcactgat cgtcgccagc tgcctgcccc aggacgaccc
     7381 catctacccg cgcctggtga ccagcggcgt gccggtgatc gcggtcgacc gcgccctcga
     7441 tccaacgcag atccgctcgg tggtcagcga cgaccagcag gccggtagcc tgctcaccac
     7501 cacgctgctc agcccgccgc cccggcatat cgccctgatc ggcgcgcgtg gcgaactgcc
     7561 gatcagccag gcccgcgaac gcggctttca ccaggccctg gatggcttcg ccgggcgtat
     7621 cagcgtctgc catgccgagc agttcagccg cgccagcggt tgccaacaga tgcacgccct
     7681 gttggccgat ccagccggag ttccggatgc cctgatcacc accgcctacg tgctgctcga
     7741 aggcgtgctg gacgccctgc aggcgcacaa cgcagaggac ttcaccgaac tgcgcctggc
     7801 caccttcggc gacacccagt tgctggactt tctgcctctg cgggtcaacg ccatatccca
     7861 gcagcacgag caaattgccg agcgagtact ggcgctggcc atcgccgcca tcgagcagaa
     7921 ccactaccag ccaggcatcg aggcgatccc gcggctgctg aaaatccgcc gtacgttgaa
     7981 ggaaaagccg ggcggcgtat gattcgccca tgcacctgat cgatacccac acccacctgg
     8041 actttcccga cttcgatgct gaccgcagcg agctgctgac ccgcagccgt aagctgggcg
     8101 tcgagcggct ggtggtactc ggcgtgtatc aggccaactg gcaacggctg tgggatctgg
     8161 tgcagggcga tgccggcctg tacgccgcct ttggcctgca tccggtctat ttggacgacc
     8221 ataagcccgc acacctgagc gagctgcgcg actggctgca gcgcctggct ggccatccgc
     8281 aactctgcgc cgtaggcgag ttcggcctgg attactttct cgaacagctg gaccgcgcac
     8341 agcagcaaca gctgttcgaa gcccaactca agctggccat cgagttcgaa ctacccgccc
     8401 tactccacgt gcgccgcgcc cacgccgcga ccatcgccac gctgaaacgc ttaaagcccg
     8461 cccgtggcgg catcatccat gcctttgccg gcagcactga agaagcccgc gaatacctca
     8521 agctcggttt caagctcggc ctgggcggcg caccgacctg gccgcaggca ttgcgtctgc
     8581 gcaaggtggt agcgcaactg ccgctggacg ccatcgtgct ggaaactgac tcacccgaca
     8641 tggcccccgc catgcacccc aaccagcgca acagcccgga acacctacca gatatctgcc
     8701 aagctttggc cgaactgcgc ggcatcagcg ccgatgagct ggcccgcgcc agcagtcata
     8761 acgcggccga attgtttggc tggaactagc tagaggacat ccgcccccac ccgcagctgc
     8821 ccaccctcaa cacgtgccag caggcgagta atgctcgggt cagccagcag gcaatcggca
     8881 attgccggct caagttcagt acgcaacctg cgccccatgg cccgggcacc gaagcgtgca
     8941 tcatgctcac ggcaaaggaa atccaacgcc gactcgtcca gcaacagcac ccgcccttgc
     9001 ttgagcaggc gctggttaag cttgctcagt tcgatctcca gcaacgcctc taaccactct
     9061 tcctcgatgt gctgaaaacc aaggatacgg tcgattcgat tgagaaactc cggctcgaag
     9121 cgccgatgca aagcgcgctc aacgacctga ttatcagcct gtgggctgag ccccagccaa
     9181 cgccgccaac cacgcgcata ccgcgcacgg taatcactga cctcgcgtgc gccgatattg
     9241 ctggtcataa agaccatgct gttacgaaag tcgagggtgc gcgtaccggc ggccagggtc
     9301 agctggccat gttcgagaat gccgagcaag ctgcgcaaca catccttact ggccttttcc
     9361 agctcgtcga acaggacgat accggggcgg ctgtagctac cctggatagc ctctgcatca
     9421 aacaacgtag tgccctcctt gctgccgaca tagcccggcg gcgcgccagt cagcgcggcg
     9481 gcatagtgct cctgggccag ggtgtgcatg tcgatacgac agaacgcatc aggtcgccca
     9541 tgaatcgcct gggccaatag ccgcacgatc tcggtcttac cgacgccggt cgggcccata
     9601 aacagattca ccgtcaacgg ccgttcgcgc tcgccgatat ccactttgag caccttgagc
     9661 aacgcgtcga cctcggccag cacggcgtcc tggccaacga tgcgcgcacg cagcagggcc
     9721 atgacttggg cgggctcgaa aacgaaacgt gacagcagcg ttgcggaccg gctctgctca
     9781 gcaaggcgct cacagttgtg ttcgatcagg tcattggcaa acggcatggg agcctcggta
     9841 gggtgcgcta tgcgcaccgc gactgttcat gtgcgcgcgg cgcaccctac gcggagcgct
     9901 ccttgccggg gtgcggcagt tcgcccaccg ggcaatccgc tacacctatg gtcttgcggg
     9961 tcagtttctc cacgtcttcc tgggcctttt gtgcgtccag cacccaggtg cggtagaact
    10021 cgaacgggca atcggccacg cccttgtcgc cgtcgccgga gttgtagacc ccggtgtagc
    10081 cacggtgcag cagcttgaac aggtggttct gcgattggtc gttggcgcgg gcgtcgcgga
    10141 tctgcgagat ggacagctgg gcgtactgga tgcccatttc ctcctcgcca cactcaccca
    10201 gggtgcgacc gtcaaagccg atcagcgccg agtggccgaa gtagctgtac acaccgtcga
    10261 agcccgcagc gttggccacc gccacgtagc agttgttggc ccaggccatg gtcttggaca
    10321 tcagcacctg ctgttccttg gccgggtaca tatagccctg gcaccgcacg atcagctcag
    10381 cgcccttcat cgcgcagtcg cgccagattt ccgggtagtt gccgtcgtcg cagatgatca
    10441 ggctgatctt catgcccttg gggccttcgc tgacataggt gcggtcgccc ggataccagc
    10501 cttcgatcgg gcaccaggga atgcacttgc ggtacttctg cacaatctca ccctgatcgt
    10561 tgatcaggat gagcgtgttg tacggtgcct tgtgcgggtg ttcttcgtga cgttcaccgg
    10621 tgagcgagaa aatgccccag gtcttggcct cgcggcaggc ggcagaaaag attgcggtct
    10681 catcaccggg gatggtcgcg gcggtggcca tcatctcgtc cttgtcgtac atgatgccca
    10741 tggtgctgta ttcgggaaac accaccaggt ccatgcccgg caggccgagc ttcatgccct
    10801 tgatcatctc ggcaatcttg cgcgcgttgt cgagcacctc ggccttgctg tgcaggcgcg
    10861 gcatcttgta attaacgacc gcgacaccga cggtgtccgg gctgctggaa atatcgccgt
    10921 ggcgcatggc tgtagatcct ccggttgact gcggtttgtg ggagcggctt cagccgcgaa
    10981 attctctctg tgtcgccgtt tatcgcggct gaagcggaat gccgcccagc agctcctaca
    11041 ggaacgccca gcagcctcaa tggctgatca tccaaggccg ggcgctgggc ttggtcttta
    11101 gcgcatgggg gttggccttg gttggcgcca gtggcttgtt cggggtgcag cagccgcaac
    11161 ctttcgggtg cttgcggctc tgcgcatatt cctcaaccgt ttgtggtgca ttggcactgc
    11221 gctcgttggc cgcaatggcg cggcgctggc tgccggacat ggtagccagg ctcggcgcgc
    11281 tgagaatcac ccgtgcgctt ggcgtggcgc agtaagggca ggtgcagggc gcatcacgtt
    11341 cggccatcgg ccgcaactgg gtgaagtcgc cacaatcggg gcaatcgtat tcgtagatgg
    11401 gcatgctggc tctcctcgga aagaccggtg cgcacggcgc accctacggt ggccgtgcgc
    11461 gcaggcatca tttgtccggt gcgatgggca ggtcgatgct gccatcgagg aacttggtcg
    11521 gaccgttggc gttggggttg atatcgaact cgaagatctc cgtcggcagc cacagggtcg
    11581 cgcaggcgtt gggcacatcg acgataccgc tgatatgccc ctgcaccggc gccgaaccga
    11641 gcaacgcata gccctgagcc ggcgagtagc cgaacttggt caggtagttg atggcattca
    11701 ggcaggcctg cttgtaggcg atattgacgt ccaggtaatg ctgcttgccg gcctcgtcca
    11761 ccgagatgcc ttcgaagatc aggtagttct tgtagttcgg ggtaatcgga ctgggcttga
    11821 acaccgggtt tttaatgccg tatttggcca tgccgccttt gatcagctcg accttcatgt
    11881 gcacccagcc ggccatctcg atggcgccgc agaaggtgat ctcaccgtcg ccctgactga
    11941 agtgcaggtc acccaccgac agcccggcgc cattcacata cacagggaag aagatcttcg
    12001 aaccacgcga caggtccttg atgtcgcagt taccgccatg ttcacgcggc ggcacggtgc
    12061 gtgcgccggt cagcgcggcc aggtcgcggg cttcaccctt catcttgccc atgtgcgcgg
    12121 tgggcgccat aggcgggttg gccaaggccg gcacgcggtg cgggtcggta tcgatcagtt
    12181 cctgctcgcg cttgttccag tcggcgagca tcttgtggtc cggcaggcag ccaatcaggc
    12241 cggggtgaat cagcccggca aagttaaccc cagggatgtg ccggctgctg gtgaacatgc
    12301 ccttgaaatc ccagatggcc ttctgcgcca tcgggaagtg atcggtgaga aaaccaccgc
    12361 cgttctgccg cgagaagaag ccgttgaagc cccactgcga gtcgggcatc gcgccgatgt
    12421 cgagcaggtc gaccaccagc aggtcacccg gctcagcgcc gtgtacgccg accgggccgg
    12481 acaggtagtg cacagtagac agatccacat cgcgcacatc gctggcgtcg tcgttgttct
    12541 tgatcgcacc ggcggtccag tcataggttt cgaggatgaa atcgtcaccc ggcttgaccc
    12601 agcaggccat cggaatatcc gggtgccagc gattgtggat ctgctcgttt tcggtggcgg
    12661 gctggttgag gtcgaccttg atcaacgttt cggtcatgac aaagctcctg ggaccattga
    12721 gggggacttg gcgccacggc gccggggtac ggagctagtc ttcaacgcgc gaggaaatcg
    12781 gcaaatacgt cggatgacgt atgccctgca tcagcgctca taaggcctgc aggatttttt
    12841 cgcggacgcg aaaaagcggc cagcgcatgg agaacacgcc agccgcaaag cttgaaattt
    12901 gagctactgc gggtgagcca ttagaccgac aggtaacggc tgatcgtcgc ttcgtcgacc
    12961 ttgtcgcggc tctcctcgac gacgaacttg cccttctcta tcaccaggaa gcggtcggcg
    13021 atttccatgg tgaacgacag cacctgttcg gacaccacga tggtcaggtc gcgcaggtta
    13081 cggatctcct tcagggtgcg ggcgatgtcc ttgatgatcg acggctggat gccctcggtg
    13141 ggctcatcca gcagcagcac tttggggttg gtggccagcg cgcgggcgat ggccagttgc
    13201 tgttgctgac cgccagaaag gttgccgcct ttacggtggc gcatctcctg cagcaccggg
    13261 aacagcgcat acaaatcgtc tggcaccttg cccttggctg aggctggcag gccagtctgg
    13321 atattctcca gtacggtcat gctggggaag atcatgcggc cctggggcac gtaggcgatg
    13381 ccgctctcca cgcgctggtg ggtctccagt ttggaaatgt cgcagccatc cacgctgatc
    13441 tgtccgcccc actgcggcag cacgcccatc aggctcttga acagggtggt cttgcccata
    13501 ccgttacggc ccatcaccgc gacaatctcg cgcttggcca cattcaggtt gaggtcatgg
    13561 aggatctggc tctggccgta gccggaggcg aggttgctga tctggaacat aaacgtcact
    13621 ccaacgtagg gtgcgctgtg cgcaccattg cgtatccggc ggtgcgcatg gcgcacccta
    13681 cgaatgcttg agccctaggg tgagccgtgc gcaccggaaa cgatgcacag ccatggcgcc
    13741 ccctgcggac gggtcaatgc cccaaataca cctcaatgac tttcgggttg ctctgcaccg
    13801 cctccatgct gccttcggct agcaccttgc cctggtgcag cacggtgacc ttgtgcgcga
    13861 tgcttttgac gaactccatg tcgtgctcga tcaccagtac cgagcggccc ttgctgatgc
    13921 gcttgagcag ttcggcggtc tgcatgcgtt cgttgacgct catgccggcc accggctcgt
    13981 cgagcatcaa tagcgccgga tcctgcatca gtaacatgcc gatctccagc cactgcttct
    14041 gcccgtggga cagcaagtcg gcctgcaggt agaggttgtc ggtaagcatg atctccgcag
    14101 caatctgctc gacccgcgca atcacctcgg cgctgcgttt gaagaacagc gcgccgaaca
    14161 ccttgcggcc cttggggtag gacatttcca agttctcaaa caccgtgagg ttttcgtaga
    14221 tcgaggggtt ctgaaacttg cgcccgactc cggcacgcac aatgtcgaac tcgcgcatct
    14281 tggtcagctc gcggttctcg aattggatgc tgccggcggt ggctttggtc ttgccgcaga
    14341 tcagatccag aaccgtggtc ttgccggcgc cgttagggcc gatcaccacc cgcacctcat
    14401 tgcggtcgat gtacaggttg aggtcgtcca ctgccttgaa accatcgaac gacacggtca
    14461 ggccctcgat ggctagcacc ggcttgggca tctgaaacgc acttggctga ctcatggggt
    14521 ggcctccagt tgtttggctt caggggcagg cgctggagtc gggctaacgg ccgcgaccgg
    14581 ggctacggat ttgcgctcaa aacgttgcag caacttttcc aggcgcttgc gcccgtggct
    14641 ttcccagacg ccggccaggc cgttggggaa gtacatgacc acggcgataa acagcccgcc
    14701 catcaggtac agccacagtt cggggaaggt ttcggaaaag taggttttgc cgtagttgac
    14761 cagcagcgcg ccgtacaccg caccgagcag cgacatacgc ccacccaccg ccgcaaaaat
    14821 aaccatctcg atcgacggca cgatgccgac gaagctgggc gacataaagc cgacctgcaa
    14881 ggcgaacatc gccccgccga tggcggaaaa cgctgcggcc acgcagaaca caaagatctt
    14941 gaagctggcc acgtcgtaac cggagaaacg cacccgctcc tccttgtcgc gcatggccat
    15001 cagcaggcgg ccgagcttgg aggcgaggat aaagcgaccg atcaggatgc agccgaacaa
    15061 caggaaggcg ttgatgaagt agaggatcat cttcgagctg tcgtcgcgga tatcccaacc
    15121 catcagggtc ttcaggtcgg tgatgccgtt gactccgccg gtcaggccct gctggccgat
    15181 gatcagcacc gtaagaatca acgcaatcgc ctgggtgacg atggagaagt acacgtcgcc
    15241 gacgcggcgt ttgaacatcg ccacaccgat caccaacgcc aaaatcaccg gcaccgcaat
    15301 caccgccagt agggtaaagc cgaagctgtg aaacggctgc cagagcatcg gcagttcggt
    15361 gatctggttc cagtccataa agtccggaat gccgggggtg gactggatct tggtgctttc
    15421 cgggtcgctg gcttcgagct tgagaaacat cgccatgcaa tagccgccga ggccgaagaa
    15481 cacgccctgg ccgaggctga gaatgccgcc gtagccccag cagagcacca ggccaacggc
    15541 gacaaaggcg taggtcaggt acttgccgac catgttcagg cgaaaggcat ccagcgccag
    15601 cgggaacacc accaggatca atgtggccag cactgccagg ccaatcgcgc cctgcttgcc
    15661 gccaagaaac ttatcaagtg tcgggttcat gggacggctc cttacttgcg cactttgatg
    15721 gagaacagcc cttgcggacg cagcatcaga atcaggatca ccgcagacag ggtgaacacc
    15781 ttggccatcg aaccgctcat aaagaactcc atcagcg
//
LOCUS       DASRUM010000158        52439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1258740, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000158 DASRUM010000000
VERSION     DASRUM010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 52439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52439
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258740"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..1687
                     /locus_tag="VFF22_10295"
     CDS             20..1687
                     /locus_tag="VFF22_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZX17239.1"
                     /translation="MADAIRLPLEVFYEREARHPNKRYMVQPLPGGQLQELSWADVGE
                     QARRAANWLRSRELPQGSRIAIISKNCAHWIVTDLAVWMAGHVSVPLYPNLTAESVGQ
                     VLRHSEAALVFVGKLDDWPAMAPGVPPDVPTIGLPLRPQGEFSFTWDDLQACTPIQDN
                     PKAAADQLATIIYTSGTTGTPKGVMHHFSNFGFAASHATELFGVGENDRVLSYLPLCH
                     VAERMFVELASIYAGQTIFFAESLDTFLEDLKRARPTVMFGVPRIWTKFQMGVYSKMP
                     AEKLDFLLKLPIIGRIVGKKVLVGLGLDAVRYALSGAAPVPEALLNWYKRLGLDVLEV
                     YGMTENCGYSHVCRPGKFKQGWIGQNSPGVEVRIAEDGEVQVRSGATMQGYYKDPEKT
                     AETITPDGFLRTGDKGEQDAEGNLRLTGRIKEIFKTSKGKYVAPAPIENRLGEHSRIE
                     QVCVVGDGMAQPMALCVLSDVGRSEALNGARGELENSLKKLLEEVNQRLDKHERLQRL
                     VLVKEVWAVDNGFLTPTLKIKRAVVEGTYGERFDEWTTRSETVLWHE"
     gene            1755..2201
                     /locus_tag="VFF22_10300"
     CDS             1755..2201
                     /locus_tag="VFF22_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015096431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HZX17240.1"
                     /translation="MALWQQTPNLEQLNAILKNTIGEQLDIRFESFDDESITASMVVD
                     ARTHQPYGLLHGGASVVLAETLGSTASHLCIDSSKFYCVGLEVNANHLRGLRSGRVTA
                     VARAVHLGRTTHVWDIRLSGADGKASCISRLTMAIVPLGENGPALR"
     gene            2498..3307
                     /locus_tag="VFF22_10305"
     CDS             2498..3307
                     /locus_tag="VFF22_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX17241.1"
                     /translation="MTQSVFFAHANGFPSATYGKLFAALAPDYSVQHLEQHGHDPRFP
                     VNDNWSNLVDELIHHLEASPQPVWGVGHSLGGVLHYHAALLRPELYLGVVMLDSPVLT
                     LADKMVIRAAKRFGFIDRITPAGRTLGRREEFVDLAEARGYFAGKGLFRRFDPECLDA
                     YVQHGLQAAGNNLRLRFDPATEISIYRSVPHTSPGRPQQLKVPLAMVRGRHSRVVMPH
                     HAHLVRRVPKGEYLTLPGGHMFPLERPQDTAELLKKLFQRWAGQPASEQSA"
     gene            3304..4167
                     /locus_tag="VFF22_10310"
     CDS             3304..4167
                     /locus_tag="VFF22_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX17242.1"
                     /translation="MSLNFEEVRLRLPHIELAAHLYGPEDGMPVIALHGWLDNAASFA
                     RLAPLLPGLRIVALDFAGHGHSDHRPPGGSYAIWDYAHDVLQVAEQFGWQRFSLLGHS
                     MGAIVSVLLAAAMPERIERLALIDGLIPYTCEPDTAPQKLGEALKAQLALAGKSKPVY
                     AEFARAVEARMRGVGAVSREAAELLAQRGLMPVPGGYTWRTDSRLTLPSPLRLTHAHA
                     LAFVRNLQCPAKLVMAQQGMLLAQPSITQLVEGLAIEVAQLPGGHHLHLDDDVGAGLV
                     ADCFKAFLAAP"
     gene            4219..4881
                     /locus_tag="VFF22_10315"
     CDS             4219..4881
                     /locus_tag="VFF22_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HZX17243.1"
                     /translation="MIDLYTAATPNGHKASIALEELQLPYTVQALSFDKQEQKAAAFL
                     KINPNGRIPAIVDRENDNFAVFESGAILLYLAEKTGQLLPSDAKGRSRVIQWLMFQMG
                     GVGPMQGQANVFFRYFPEKLQGPIDRYQHETRRLYEVLDTRLGESQYLADDYSIADIA
                     TYPWVRIHDWAGVSVDGLEHLQRWMAELAARPAVQRGLLVPQREADGEKVVKAAQAML
                     IK"
     gene            4878..5690
                     /locus_tag="VFF22_10320"
     CDS             4878..5690
                     /locus_tag="VFF22_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4892 domain-containing protein"
                     /protein_id="HZX17244.1"
                     /translation="MSRLNRYCLGLSLLLSSAVVWADVAGSQDLDVLPRFAGSKIVSF
                     KDVAEQERIYPQGAIRRISGTLRYEREVAAQGQLTSVTYELPRTHTANEVFAKARGDL
                     QAQDAELLYWCVGRECGSSSLWANAVFTNATLTGSDDQQTYALLRLAEPRQDSLLALY
                     SITRGNKRAYLHAELLTANAPLGDVLPTPATLQRQLKSTGELRLATQVEPSAAWVELL
                     ARSLKLDSTLRVIISGAQAEAWREALVEQRVRASRLELGEADVNGVQLNVLR"
     gene            5782..6861
                     /locus_tag="VFF22_10325"
     CDS             5782..6861
                     /locus_tag="VFF22_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007244757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZX17245.1"
                     /translation="MANNDRLLVQILLLVLLGASLWVLAPFWSALFWAAVLSFASWPV
                     MRVLTRLLGGRQALAAGLLTLAWTVVVAVPLVMLGFNLADHIKDVTALLKDFQVDGLP
                     PAPTWLAGVPLVGERLVGFWMTIDQQGAAFFATVRPYLGLVGNWLLARSAQIGGGMLE
                     LALSLILVFFFYRDGPRLAAFAESLLQRLIGDRAEHYLELVAGTVQRVVNGVIGTAAA
                     QAILAWIGFTIAGVPGALVLGILTFGFSLIMVPPLIWGPAVAWLFWQGEVGMGFFLLV
                     WGFFVISGVDNILKPYLISRGGNLPLIVVLLGVFGGILAFGFMGLFLGPTLLAVAYSL
                     LSDWVGSPVVNTAASSAEPTDKPLN"
     gene            7103..8647
                     /locus_tag="VFF22_10330"
     CDS             7103..8647
                     /locus_tag="VFF22_10330"
                     /inference="COORDINATES: protein motif:HMM:NF017870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HZX17246.1"
                     /translation="MKKIAGSALGAVVVLGALSTAGAWYTGQQLPAVLETSIQQANQD
                     MGKTLPALGLSATIELVSLERQLFSSNARYRIQFAGSLDGESPSNFEWFVTDRIEHGP
                     FPLSRLQTLKLLPVMATSNYELERSPELEKWFAGSQGGAPLQGQVSLGYDRSLSGNLR
                     VQPLQVALDEQTNIDFSGLEIDFDSSANVEKISASGVMDSFKLTSTLSTDESMRLEFK
                     GMDLTSNTRKGVSDFYLGQNEVRLQQIELQIGESAPILFKDFVQRDETSEANQQLSAR
                     YSYDVGMISYQGNDIGSSQMLWTVKNLDAAALQSMIELYGTLLQSGKVDSESGMPELT
                     DEQTALFKTDLEKLLAGKPGLALEKLAFKTANGESSFSFGLDLNKPVSFDLPAPELAK
                     QLIAQLDAKLLVSKAMITDVIGVQATLAGETDKEAIAQQAAMVGEMASGMAVASELAV
                     LQGEDISSSLHYANDQVTFNGKQMTVDQFVALAMSSGGGLAGMGHETPADEVLLDESE
                     GESTIQ"
     gene            8858..8947
                     /gene="kdpF"
                     /locus_tag="VFF22_10335"
     CDS             8858..8947
                     /gene="kdpF"
                     /locus_tag="VFF22_10335"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009618573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HZX17247.1"
                     /translation="MSILDGVSLGLALGLFIYLLVALLRADQA"
     gene            8956..10656
                     /gene="kdpA"
                     /locus_tag="VFF22_10340"
     CDS             8956..10656
                     /gene="kdpA"
                     /locus_tag="VFF22_10340"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HZX17248.1"
                     /translation="MHSYDFTLILAFFVLVLLPAPFIGRYLYRVMEGEKTWLSPVLQP
                     VERLCYRIAGVDSNREQDWKTYSLALLAFTLISLLTLFAILMLQGSLPLNPQQLPGLE
                     WTLAFNTAVSFVTNTNWQAYSGEAQMSYFSQMVGLGVQNFVSPAVGLAVLVVFCRGIA
                     RRSSNSVGNFWVDLTRATLYGLLPFCLVLALLLVWQGVPQTFLEYVTAHTIGGTDQVV
                     PLGPAASQIAIKQLGTNGGGFFGVNSAHPFENPTAWSNLFEVASIILIPAALLFTFGH
                     YVKDLRQSRALLACMLILFSVGLGGTLYAEHQPNPALAALPIEQSGSLEGKESRFGTT
                     ASALWAVTTTAASNGSVNAMHDSFSALGGMIPLFNMMLGEVIFGGVGAGLYGMLLFVL
                     IAVFLAGLMIGRTPEYLGKKLEAREVRLLVATLLVMPIGVLVFGALAASLPGPAASVT
                     NPGAHGFSQILYAYTSGTANNGSAFGGFGANTPYHNLMIGLAMLLGRFGYILPILAIA
                     GSLAAKKRAPVGSNSFPTHGPLFVTLLTLTILLVGGLTFLPALALGPIAEHMTLFQGF
                     "
     gene            10669..12729
                     /gene="kdpB"
                     /locus_tag="VFF22_10345"
     CDS             10669..12729
                     /gene="kdpB"
                     /locus_tag="VFF22_10345"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HZX17249.1"
                     /translation="MNAQTQVKSPALNSAQQHPKTSLAALWKPALRQAFVKLDPRQLP
                     RSPVMLVVELTAVVTTVLCFIPNPQVSTGLAVQIALWLWFTVLFANFAEALAEGRGKA
                     RADSLKAGSQGLNARRKNGQQFETVAASSLRRGDIVRVEAGELIPGDGEVVEGIAAVN
                     EAAITGESAPVIRESGGDRSAVTGNTRVVSDWLLVQITANPGESTLDRMIALVEGAKR
                     QKTPNEVALDILLIGLTLIFLLVVATLQPFARFAEGDLPLVYLVALLVTLIPTTIGGL
                     LSAIGIAGMDRLVRLNVIAKSGRAVEAAGDVHVLLLDKTGTITFGNRRCSALYKAPGI
                     SGKQLGDAALLASLADDTAEGKSIVEYLRALNTMVEPERSAIKSIAFSAETRLSGADY
                     NGHSYRKGAVDAVLLYLGLQRSDVPPVLAREIEIIAQSGGTPLLVAGDGQLLGAIHLK
                     DVVKPGIRERFAELRAMGIRTVMVTGDNPLTAAAIAAEAGVDDVIAEATPEKKLQRIR
                     SEQAEGKMVAMCGDGANDAPALAQADVGLAMNDGTQAAREAANLVDLDSDPTKLLDVV
                     QVGKELLVTRGALTTFSVANDVAKYFAILPALFAGIYPQLGALNLMQLHSPQSAILSA
                     IVFNALIIVALIPLALRGVRVQAADAASLLRRNLLIYGLGGLLAPFVGIKLIDLLLVA
                     AGLV"
     gene            12815..13381
                     /gene="kdpC"
                     /locus_tag="VFF22_10350"
     CDS             12815..13381
                     /gene="kdpC"
                     /locus_tag="VFF22_10350"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HZX17250.1"
                     /translation="MFKQIRPAISVLAFMTLVTGVVYPLTVTGVAQLAFPAQANGSLL
                     HNQQGDVLGSALLAQPFEGAQWFQPRLSAGGYATVASGASNLAPSNPALAERIATDAQ
                     RLQVEDQGAVPMQLVTTSASGLDPHLSPAAAQFQIARIAAARGIPAVSLERLIEQHVE
                     KPLVGPAVVNVLALNLALADNSPPAKAE"
     gene            13387..16041
                     /locus_tag="VFF22_10355"
     CDS             13387..16041
                     /locus_tag="VFF22_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HZX17251.1"
                     /translation="MSDAVRADALLDEMPREGRGRLKVFLGAAPGVGKTYAMLQAAQA
                     QLRQGVDLRVGVVETHGRAETEAMLTGLPQQPQQRLEYRGVALSEMDLDGILAKPPKL
                     LLVDELAHSNAPGSRHSKRWQDVQELLDAGIDVYTTVNVQHLEALNDQVRDITGVQVR
                     ETLPDWVLQEADEILLIDLPPRELLERLREGKVYVPEQARAAIDAFFSQTNLTALREL
                     AMQTAAARVDADLSRRYRQQGQQAPALRGRLLVGIDGDAQAERLVRHACRVAERRHLP
                     WSVVYVDTGGERDETRLSYLQGAQQLAERLGGEVVSLRGSEVAKILIEHARERRASLI
                     LVGRSRQRLRRIWHLGRGLAERLLQQGQGLEISVLDTEASNAPALSRRRQPVVWRDYL
                     LALVATLLASVVALGLSRVLELPNISLILLAAVLVVAVRSSLGPAMACAGLSFLAYNF
                     LFIPPTLTLTIDRQQDVLTLLFFLLMAGLTGNLASRQRRQLQALRETQRETSALLELS
                     RKLTAATDRQAVLAAAMQQLGLWQDVEVSLLSRSPDALWKVEAGAQRLLTDLERAAAD
                     WSWQHDQPAGLGTGTLPDGRWWWLPLSGEEGPLLLLGVSPKDGRALPAERRRLLTALG
                     QPLAQALERAQLAEDLEAARLHGQTEELRSALLASVSHDLRTPLTAMRGSIDSLLALG
                     EQIPLADRRELLEGTRDEAERLDRYIQNLLDMTRLGHGGLKLARDWVAPADIVASATQ
                     RLRPVLSALKLEIQVPQELPLLYVHAALIEQALVNVLENAARFSPPQGRLRVAIEADT
                     AELRFIVSDQGPGIPEHERTKIFDMFYTAARGDRGGQGTGLGLAICQGMLGAHGGRVT
                     VGEGLDGLGASLTLHLPLHTQPQMEEEQ"
     gene            16052..16747
                     /locus_tag="VFF22_10360"
     CDS             16052..16747
                     /locus_tag="VFF22_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX17252.1"
                     /translation="MSQNPATILVIDDEAQIRKFLRISLTAQGYKVLEGATGQEGLAQ
                     AALGKPDLVVLDLGLPDMDGQQVLRELRAWSQVPVLVLSVRASEGEKVLALDGGANDY
                     VTKPFGIQEFLARVRVLLRLAGGVEQASAAVSSGPLQVDFAYRRVSLDGVEVSLTRKE
                     YAVLAQLARHIGRVVTQQQLLKDIWGPTHGEDSHYLRVVVGHLRQKLGDDPTAPRFIV
                     TEAGVGYRLRDSA"
     gene            complement(16892..17917)
                     /locus_tag="VFF22_10365"
     CDS             complement(16892..17917)
                     /locus_tag="VFF22_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZX17253.1"
                     /translation="MSKRIALVLGSGGARGYAHIGVIEELEARGYEIACIAGCSMGAV
                     VGGIYAAGKLNEYRDWTQSLDYLDVLRLLDVSFRLGAIRGEKVFGKIREIVGEINIED
                     LAIPFTAVATDLTNQQEIWFQEGCLHQAMRASAAIPSLFTPVVQGKRMLVDGGLLNPL
                     PIVPVVSAHCDLIVAVNLNSAHQRHYELPIIERTPALKGRFDLLMSSLGSRLPSFKRK
                     PGEDDGLLLLEDQNHVMQPEAINPWQQNTRGSLDKPAAPKSASGSRVADLSGPASLLE
                     LINQSFEVMQTSLSQYKIAGYPPDILINVPKRVCRFFEFYKAPELIMLGRQIASDTLD
                     KYERGDR"
     gene            18172..18450
                     /locus_tag="VFF22_10370"
     CDS             18172..18450
                     /locus_tag="VFF22_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17254.1"
                     /translation="MKLWIVVPMLLLVLSGCAGKTAYRDSCANQLDAAWRELDLAKAE
                     GFAGTVSYSKAVSLMTAAKTQQQFEAYKGCTAKSERARFYIRESRAGR"
     gene            18450..19058
                     /locus_tag="VFF22_10375"
     CDS             18450..19058
                     /locus_tag="VFF22_10375"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZX17255.1"
                     /translation="MQLDFSALEPLERYRWLASTITPRPIAWVSSLSAEGVSNLAPFS
                     FFQVISDDPPTLMVNVNHRADGGLKDTLLNVQASGELVIQLVSFAQAEAMNASAAVLP
                     YGISEFEQCGIASQPSQRVATPRVAGAAVAFECQVAQIQAYPADKPNCHLIFAEVLLA
                     HIDDAVLNEQGRIDAHKLDLVGRLGGMAYSRTRDTFEMRRPG"
     gene            19241..21205
                     /locus_tag="VFF22_10380"
     CDS             19241..21205
                     /locus_tag="VFF22_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003217188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX17256.1"
                     /translation="MWTVVQRQVASLSTASKLALGFGIVLALTALVAATGFLALRDVS
                     ARSTLLERMSAINSQVMQIRRSEQDFVLSSDKQHATRLHEQAQQILDASAALQAQLPQ
                     AERVGLDQVAEAMTAYRAAFDRYVEQSDNMHLSLEAATWLVVSASNSLDLLKDGLAED
                     GVDLLKSSQGQQGAESVLQAGQISQVHQLLLRALDQARVLLEQSRSSGEAIPTRIQEA
                     ADAQALAGELRDAMEDPGYAAVLAEVIGNVNSFNDRLGEYTGLLQQQKREYQNLVQQA
                     ERILALVDQAYARQKASLDQQLQTSTWLIVLATALALLIGLLATLTITLLIVRPLKQV
                     IEQARRIADGDLSARIEVQRGDEIGQLQAAMQGMSSNLREMVGSLQGGVLQISASAQS
                     LSASTEQTRLGVNEQKAETDQVATAMSEMTATVHEVARNAEAAASSTEQADRRVNSGV
                     TVVQQTLARINQLASAMQTTTQSIERLSQDTQRIDSVLDVIKSVAEQTNLLALNAAIE
                     AARAGEQGRGFAVVADEVRALARRTQQSTTEIEGLIATLREGARRSVADMHQSGVLVT
                     QTVEDANQTEGALAEIAAAVSQIFEMNQQIAAAAEQQTAVAEEISRSVTSIRDIADQS
                     ALAMDESASSSIQLAELGRELQGMAGRFRL"
     gene            complement(21269..23956)
                     /locus_tag="VFF22_10385"
     CDS             complement(21269..23956)
                     /locus_tag="VFF22_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HZX17257.1"
                     /translation="MQAPSGHDWHALPGQQSLQHLNSSAQGLSSADAAQRLSEHGPNL
                     LPQQQASGPLKRFALQFHNLLIYVLLGACLLTLLLGEWLDSAVIFAVVLINALVGFVQ
                     EGKAQQAMLAIQQMLSLESRVRRNGQVHSIAAEQLVPGDWVLLEAGDRVPADLRLLET
                     RGLRIDEAALTGESLPVDKHQDAVDAATSLADRRCMAYSGTLVNAGSAAGLVVATAKQ
                     TELGKISHMLENVVSLQTPLLVDMAKFARHLTLGILILALLTFAFGVLLRGYSAEDML
                     LAAVSLAVAAIPEGLPAVLTIILALGVQRMAKRRAIIRHLPAVESLGAVTVICSDKTG
                     TLTRNEMTVQQVFTAAHHYSVDGVGYAPQGNIHCDDGLLCQLDAADDLRELARAGLLA
                     NNASLRSGENGWCITGDPTEAALLTLAGKVGLELQHEASNLPQVDAIPFSSEQRCTAS
                     LHHDHARHGLIYMIGAPERVLEACNRQWRDGHPEPLDPRHWHQALDSGASQGLRMLGL
                     AIRPLGEPQQSLDYQDLDGDYVLLGLVGMLDPPREEAIAAIADCHSAGIAVKMITGDH
                     AGTASAIAQRLGLGDGKPLTGADLDSLSDSQLDQLLPATNVFARTSPAHKLRLVERLQ
                     ACGARVAMTGDGVNDAPALKRADIGIAMGIKGTEAAKEAAQMVLADDNFATIAHAVEE
                     GRTVYDNLKKTILFILPTNGGEAMILLAAIALGLTLPITPLQILWINMITAVTLALAL
                     AFEPAEADLMRRPPRNPKVNLLSQHLLWRVGFIAVLMTLASLGLFLYTQMLGWSLESS
                     RTLAVNALVACEIGYLFNSRRLEGPANFGLRDNPMIWSMVGLLLSLQLLLTYWPAMQQ
                     VFATAALPAFAWGWCLLVALGVCACVEAEKWLLRRLKRS"
     gene            24126..25043
                     /locus_tag="VFF22_10390"
     CDS             24126..25043
                     /locus_tag="VFF22_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZX17258.1"
                     /translation="MRAKLETVLQTVNQVLLGKEPQVRLALTCLLAQGHLLIEDLPGM
                     GKTTLSHALARVLGLSFQRIQFTSDLLPGDILGTSVFDKDTGQFIFHPGPIFAELVLA
                     DEINRATPKSQSALLEAMEEGQVTIEGATRPLPEPFFVIATQNPVSQGGTFALPESQL
                     DRFLMRLSLGYPGKAAEKALLLGDSRRSLLPTLPALLDHAELALLQAEVPKVRASDAL
                     IDYVLRLVDATRTQPQFAWGLSPRASLALLAAARAWAFLAGRDYVIPEDVQAVLPSVV
                     GHRLRERADPAGHGAGSLVQWLLREVPAL"
     gene            25040..25996
                     /locus_tag="VFF22_10395"
     CDS             25040..25996
                     /locus_tag="VFF22_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZX17259.1"
                     /translation="MIAQLKPRWNRWLARRIPAAASVRLNQRRIFIIPSRVGAAFMLA
                     LLLMLLAAINYQNSLAYGLTFLLASVFVVAILHTYRNLAGMILKAGSAPAVFVGEPAG
                     FRVRLESSQRPHQAVSLGWPPAELQTLDVPAEGVSECQLNLPALQRGWLRPGRLRVES
                     RFPLGILVAWSWVDLDQRVLVYPRPLPGDLPLSAGASEDQDEEGSRPHGQGVDDYQGL
                     KTYQPGDSKRRLHWKAYSRGQGLLVKDFASITGRDLYLDFEAVAGDVETRLSLLCHWV
                     LQLSERQQPFALSLPGLVLGADSGELHRDACLRALALFGRAP"
     gene            25993..27987
                     /locus_tag="VFF22_10400"
     CDS             25993..27987
                     /locus_tag="VFF22_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HZX17260.1"
                     /translation="MSRIAVIPRISLTWLLVAQVLVIIPHLTHLPLWIIGLWLGSAAW
                     RIQIFRMRARYPNAWAKGGLMLAAALGVFLSRGSLIGLDAGVVLLIAAFVLKLLEMRT
                     RRDALVLIFLGFFVVVTAYLFDDSMLMAFYSLLPVTALLAALIGLQQSSLAIQPWPTA
                     RLAGGLLLQALPVMLLLFVFFPRMGPLWSLPVPNDKATTGLSDSMAPADIADLSRSAA
                     LAFRASFNGETPPRSELYWRALTLDRFDGRRWSQSEYAKLGPAAQWQKRGEALSYSVV
                     MQPSAQRWLFALDVAETTLEQARQMSDFRLQRRQPVDRSLIYQATSWPQGLREPQLPQ
                     AAQRRALQLPDQGEPRSRAWAEQLKREHPQPEELVQALLSHFNREPFAYTLTPPTLGV
                     NNIDDFLFETRSGFCAHYAGAMTYVLRAAGIPARVVAGYQGGEFNPAGNYVQVRQFDA
                     HAWVEYWQEGQGWVSVDPTFQVAPERIDMGLEEALAGEQSFLEDSPFSPLRYRNLSWL
                     NELRLAWDNINYGWQRWVLGYQSQQQLEFLQRWLGRVDAQSLVIGLVGGGALLLGLLS
                     LWLFKPWQRERDVQQRLFRRFEGLLARHGVPRKPGEGPRDYATRAALLMPTQAQSIGD
                     FAAAFEAQRYAGQEPSALQLRQHLKALRRALPWRFTRLDS"
     gene            28057..28827
                     /locus_tag="VFF22_10405"
     CDS             28057..28827
                     /locus_tag="VFF22_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019360337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HZX17261.1"
                     /translation="MQDLIDEVPRHVLRIQMRLFACRERLRAGTDDEALHDLRIALRQ
                     LRSLLRPLSSVAVCEDLQQRAAELGRLTGPLRDLEVLQGYLQQQGLDSAAQSRLPLLQ
                     QGYLQLLDSAPLAHLLNALDDWPQQWRQAANNGQLRGLHKLIKQRLRKDHKHLAQALL
                     DPAHDRHRLRLLVKRLRYAAEAYPQLADLSGRMRMQLKQAQSALGDWHDHWQWLLRAE
                     AEADLQPCMPAWREAMQSAEQQADLALQALIAGFARSG"
     gene            28935..29732
                     /locus_tag="VFF22_10410"
     CDS             28935..29732
                     /locus_tag="VFF22_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZX17262.1"
                     /translation="MGFSELLQAVRDNPQAVMIPAQWGQGRASFGGLVTALVYEAMRA
                     QVPSGRPPRSLAITFVGPAAPDVPVRFEVEVLREGKAVSQVLGRAIQDGQVVTLVQGS
                     FGAGRESRIAVQADPAPQAKAVEDCPELPYIPNVTPEFTRFLAMRWAFGGMPFTNTPS
                     REMGGWVRLRDQGVAEAANEAHLLALVDAWPPAVLPHLSAPAPGSSLTWTIEFVQPMS
                     DLSTLDWCLYRAQIEHARDGYGHVAAALWSPAGELLAISRQTVAVFG"
     gene            complement(29729..31195)
                     /locus_tag="VFF22_10415"
     CDS             complement(29729..31195)
                     /locus_tag="VFF22_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX17263.1"
                     /translation="MGHWISDLSLKYKFWAVNAVAFVTTLLLVLYALQLEQQVHHTAA
                     KQAAQQQARLLSQWPQEQPLPSASNLLSVAAGQTPRLPDNQGSALANARGWVDLNGSD
                     GLIGAEVIVREDGQHLAITASAPSLSEVFFDRAPSYALVVALLMLLLLAASQLLIRFL
                     LSHLNTLKDVMLQVEKSGDLSARVPLDCKDEVGQMASAFNAMQAGYQRVVGIVAQSAA
                     QLDEGAERLAGNMRDVRQGMLEQQSETEQAATAINEMSATVHHIAEHTADTRDQSKNA
                     EQLARSGQQAVNRVSLSISGLSSGVQQTAQMIEQLAVDSQKIGGVVSEIHGIAEQTNL
                     LALNAAIEAARAGEMGRGFAVVADEVRNLAKRVQDSTDVITQMINTLQSGTRDAVDFM
                     QDSSIKADECVKQAGEAGQALVAITESVALMRESNTQIAVAAEQQSQVAEEMNRAVIR
                     IRDVTEQTVGQTGQSAITSADLANLAGELNKAIRQLKL"
     gene            complement(31311..32129)
                     /locus_tag="VFF22_10420"
     CDS             complement(31311..32129)
                     /locus_tag="VFF22_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017339820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZX17264.1"
                     /translation="MQLIDIGVNLTHPSFAEQREALLARAYAAGVQQMLLTGTSLTES
                     QHALELCRQLDDRAEHLFSTAGIHPHDASSWNSDSHAQLKALLANPRVRAVGECGLDF
                     NRDFSPRSQQELVLEQQLALAVELQLPVFLHERDADERLLAILRHFRDQLPAAVVHCF
                     TGEKRALFNYLDMDLHIGITGWICDERRGSHLHPLVKEIPRGRLMLESDAPFLLPRSL
                     RPKPKSGRNEPAFLVEVLREVALHRGETEQVLAAHTSAAAQAFFKLPAFKGTAE"
     gene            32365..33750
                     /locus_tag="VFF22_10425"
     CDS             32365..33750
                     /locus_tag="VFF22_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018929805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZX17265.1"
                     /translation="MLRVLLTCCLLLLSCTAVARVAGPLEVEQPKAARDLASIRNSGE
                     LRVLVNQSRNSSGEVKGQSIGVEYQRMRAFEQHLNRNSRDGRTLKLKIIPRAKDQLLA
                     ALQRGEGDLVAPGELLNMPRGSKLSASAAIRSNVPMIIVAKQGNRRYQNLEQLSGRSL
                     ALPAGSVAGEAVRAVNQRLIDSKRSPIIVEWVDPSLAVEDVLEMVQAGIFSVTAVEQP
                     IAERWAKVMPKLRLDRHLQLTNDGDMRWYVRRDTPMLGASIDRFLKGYRDPADQDAAF
                     QRVYRRLYKVHYPLGRTERQRLEKVRPVLQRYANQHDFDWLALAAVAFKESTLNPAAR
                     GAGGATGLMQITPAAARSVGVSNISVLDNNVQASAKYLAMIRRNYFNSPQLNERERMA
                     FILAGYNMGPQRVQSLRAEARRRGLNPNQWFFQVERVAMEQLGMGVVSYVNSVNKYYL
                     AYDRERYLLEP"
     gene            33876..34310
                     /locus_tag="VFF22_10430"
     CDS             33876..34310
                     /locus_tag="VFF22_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZX17266.1"
                     /translation="MNALIKSVVATQAGYGLTVLRIIAGITFAAHGAQKLFGWFGGYG
                     LEGVGQWMESIGLAPGYLLALMAGSAEFFGGIALIIGLLARPAAAVLALTMVVAIATV
                     HLANGFFMSNNGYEFALALLAISVAILIEGAGKLSLDKRIAG"
     gene            complement(34362..35387)
                     /locus_tag="VFF22_10435"
     CDS             complement(34362..35387)
                     /locus_tag="VFF22_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HZX17267.1"
                     /translation="MKKLLLLSPLVFAACLALALYLQPVDIPAQLGADTPAPAVESAD
                     SAPANQTQHKPKNAADPLKTLPASFGGTTVDGVFRLDADGNLLISEDIRRIFDYFLAA
                     VGEEPLRTSVERLRAYIAAQLPANAREQALALLNQYLDYKRELVLLERDLPQMANLDA
                     LRQREAAVQALRARIFDAQTQQAFFAREEAYNQFTLQRLAILHNSQLDDAGKAAAVDQ
                     LRNSLPAEMQDTVLPQLQNELRQQTAQLQAAGAKPEQIRQLRQQLVGAEATQRLEVLD
                     QQRQTWKKRIAQYSTEKAAIENNTGMSASDKRAAIAQLAAENFDEREQLRLEAAEQLA
                     TQNKPQP"
     gene            complement(35463..36407)
                     /locus_tag="VFF22_10440"
     CDS             complement(35463..36407)
                     /locus_tag="VFF22_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
                     /protein_id="HZX17268.1"
                     /translation="MNNNKTLLALCLGAGLLASGNTHAFWFGSSGYTQTKYPVVLAHG
                     MLGFDSLLGVDYWYGIPAALRRDGASVYITEVSQLNTSEARGEELLAQVEDIVAISGK
                     PKVNLIGHSHGGPTIRYVAAVSPNLIASATSVGAPHKGSATADFIRQVPPGSAGETLL
                     AGIVNGLGTLINFLSGSSSTSPQNALGSLESLNSQGAARFNAKFPQGIPTSACGEGAY
                     SVGGVRYYSWSGTSPVTNIIDPSDLLLGATALTFNGEANDGLVGRCSSRMGKVIRDNY
                     RMNHLDEVNQTLGLTSLFETDPVTVYRQHANRLKNDGL"
     gene            36952..37260
                     /locus_tag="VFF22_10445"
     CDS             36952..37260
                     /locus_tag="VFF22_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003172695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX17269.1"
                     /translation="MHCPQPLIRLAPITSGLTLRNPKILLGGSHQPTLLRYLDGWPKR
                     WPGPRTFLINFVHDGESLARFASNHFDMAVIEAPAAAQLEETVRQLTRVAKQGLITRR
                     "
     gene            37335..38822
                     /gene="pap"
                     /locus_tag="VFF22_10450"
     CDS             37335..38822
                     /gene="pap"
                     /locus_tag="VFF22_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HZX17270.1"
                     /translation="MFESAEIGRSIDKETYEAEAPALREALLEAQYELQQQGRFAVLI
                     LIDGIEGAGKGETVKLLNEWMDARLIQVNTFDQQTDEELARPPAWRYWRQLPPKGRIG
                     IFFGNWYSQMLQGRVHGRIKNAVLDQAIDSTLGLERMLCDEGTLIFKFWFHLSKQQMK
                     ARLKALQDDPLHSWRISPLDWQQSKTYDKFVRVGERVLRRTSRDYAPWYVVEGVDEHY
                     RSLTVGRILLDGLQAALKAQSQPLPHPHAAPLTSSLDERGLIDSLDMTQALDKDSYNE
                     QLVIEQARLSSLMRDKRMRRHSLIAVFEGNDAAGKGGAIRRVIGALDPRQYRIVPVAA
                     PTEEERAQPYLWRFWRHIPPRGKFTVFDRSWYGRVLVERVEGFCSTGDWLRAYGEIND
                     FEEQLSEAGVVLVKFWLSIDQQTQLERFKAREQIPFKRFKITEEDWRNRDKWPLYRDA
                     VGDMVDRTSTEIAPWTLVEANDKRFARIKVLRTINQALEAAFAKG"
     gene            38943..40127
                     /locus_tag="VFF22_10455"
     CDS             38943..40127
                     /locus_tag="VFF22_10455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZX17271.1"
                     /translation="MREVVIVDSVRTGLAKSFRGKFNLTRPDDMAAHCVNALLARTGI
                     DPALVDDCIVGAGSNEGAQGMNIGRNVAVLSGLGTNVAGMTLNRFCSSGLQAIAIAAN
                     QVASGCSDVIVAGGVESITMTMKSMNTQNLFNPLLQKDVPGIYYPMGQTAEIVARRYN
                     VSREAQDAYALQSQQRTARAQAEGLFDDEIVPMHVKYQVEDKATGEKSIVDGIVAHDD
                     CNRADTTLESLAALKPVFADDGSVTAGNASQLSDGASMTLVMSLEKAIELGLKPLAFF
                     RGFVAAGCEPDEMGIGPVFSVPKLLKAKGLSVADIDLWELNEAFASQCLYARDRLEID
                     NAKYNVNGGSISIGHPFGMTGSRQVGHLVRELQRRNLRYGIVTMCVGGGMGATGLFEA
                     VR"
     gene            complement(40203..40412)
                     /locus_tag="VFF22_10460"
     CDS             complement(40203..40412)
                     /locus_tag="VFF22_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6316 family protein"
                     /protein_id="HZX17272.1"
                     /translation="MFGQRHIDHNPGTHYRSERVSAVNGQYFFSTREGTLEGPYFNRT
                     DAEREIAFYIRRMIQANEIIESRAF"
     gene            complement(40650..41270)
                     /locus_tag="VFF22_10465"
     CDS             complement(40650..41270)
                     /locus_tag="VFF22_10465"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZX17273.1"
                     /translation="MSEAAKSGATPLEGDGKRILMILGTPKNSSFCHALGEAYAQGAR
                     SKGHVVRQLKLGEMSYDPVLRDGYEQSQTLEPDLLEAQRQIHWAEHLVFVYPVWWGGI
                     PALLKGFFDRTFLPGFAFKYRNRSQLWDKLLTGRTADLLVTMDTPPWYFRWIYGAPGH
                     RQMTRTILGFSGIKTRRLAEFAPVRPSSEEQRQSWLRRAEALGVRA"
     gene            complement(41263..42657)
                     /locus_tag="VFF22_10470"
     CDS             complement(41263..42657)
                     /locus_tag="VFF22_10470"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HZX17274.1"
                     /translation="MSRTETDSLGPIEVPDEAYWGAQTQRSLVNFAIGQERMPLPVVH
                     ALALIKKAAARVNQRIGEVPADLARLIEQAANEVLAGQHDEQFPLVVWQTGSGTQSNM
                     NVNEVIAGRANELAGNGRGGKTPVHPNDHVNRAQSSNDSFPTAMHIAAVQAVQQQLLP
                     AVRELRDGLQEQAERHARLVKTGRTHMMDATPMTFGQELSAFVAQLGYAERAVQAVLP
                     AVCELAQGGTAVGTGLNSPQGFADAMAAELAALSGLPLVSAPNKFAALSGHEPLVALS
                     GALKTLAVALMKLANDLRLLGSGPRAGFAEVRLPANEPGSSIMPGKVNPTQCEALSML
                     ACQVLGNDTTIAFAASQGHLQLNVFKPVIIHNLLQSIRLLADGCHNFQQHCVAGLQPD
                     AAKMAEHLERGLMLVTALNLHIGYDKAAQIAKKAYEDGTTLREAALQLGYLSNEQFDA
                     WVRPENMLEAGQHE"
     gene            complement(42777..43787)
                     /locus_tag="VFF22_10475"
     CDS             complement(42777..43787)
                     /locus_tag="VFF22_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HZX17275.1"
                     /translation="MNSFTHPEPLAAIALCDSHGLLNPQAIGWSPRPMVDCAIPGHFG
                     RRKRWNHWCITTPQWMLSFTQADLDYLGYAAAYFLDLQSGQAVAYSQYRLFGLGCDLP
                     DQPQSSHEFNHPRLQLRVAEHTGRTRITINAPDIGGQPLQVALDIQRPAHLDSVNLVA
                     PLGPHSFHATSRQLGLPCSGSVQLGKQHYSCVTGQSFASLDFGRGVWPLNSHWTRAAF
                     AAPGGIAGNFGSGWTDHSGLSENTLWFGGELQHLDTPITITQAPHNPLAPWQLRSPDD
                     AVQLTFTPRQRHQAYRRLGPFYADTQQWFGHFEGVLRGPEGERVPVNNALGWLGETRA
                     RW"
     gene            44053..44553
                     /locus_tag="VFF22_10480"
     CDS             44053..44553
                     /locus_tag="VFF22_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HZX17276.1"
                     /translation="MSRLPAFCAALILTCGSAQVLADAKSHAADAERFLLLARADKLA
                     VPVYAQVQQMFAQRFAESNAPQSEKAVLETYQAQANAALEQAVGWDKLKPDMVKLYTS
                     NFNEQEMKDLIRFYESPLGKKVLEKMPTLTAQSAQLTQGKLKGAVPKVNQLLAEMTTK
                     LTPKKP"
     gene            44566..44859
                     /locus_tag="VFF22_10485"
     CDS             44566..44859
                     /locus_tag="VFF22_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HZX17277.1"
                     /translation="MPKIDLLQAALASLQPQHLDVLDESHMHSRGLETHYKAVIVSEQ
                     FAGLNAVKRHQKVYAAVGELMGQIHALALHTYTPDEWSQQGVAPASPTCKGGH"
     gene            45129..46067
                     /locus_tag="VFF22_10490"
     CDS             45129..46067
                     /locus_tag="VFF22_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009045022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HZX17278.1"
                     /translation="MTDKIVVAALYKFVSLPDFHALREPLLQCMLDNAVKGTLLLAEE
                     GINGTVSGSRAAIDGLMAWFGLDPRLADIDHKESYCDEQPFYRTKVKLKKEIVTLGVP
                     GVDPNQKVGTYVEPKDWNALISDPEVLLIDTRNDYEVSIGTFEGAVDPKTKSFREFPE
                     YIKAHFDPAKHKKVAMFCTGGIRCEKASSYMLGEGFEEVFHLKGGILKYLEEVPQEET
                     KWQGDCFVFDNRVTVRHDLTEGDYDQCHACRTPVSVEDRQSEFYTPGVSCPHCWDSLP
                     EKTRAGARERQRQIELAKARNQPHPLGYNPRQSREA"
     gene            46070..46702
                     /locus_tag="VFF22_10495"
     CDS             46070..46702
                     /locus_tag="VFF22_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018929864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HZX17279.1"
                     /translation="MQARLLYVMDPMCSWCWGFAPVLESLAEQAAAAGVGLQLVLGGL
                     RRDGVAIDAAARVRYLGYWQAVNASTGQLFNFNEGLPEGLVYDTEPACRALVTARSLA
                     PELAWPLAQLIQRAFYTQGVNVTRASVLVELAERAGIPRIVFAEAFDSAAQRAATAAD
                     VSWVQDLGIAGFPTLLAERNGQMALLTNGYQPLEVLQPLLARWLERAAHA"
     gene            46695..48473
                     /locus_tag="VFF22_10500"
     CDS             46695..48473
                     /locus_tag="VFF22_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX17280.1"
                     /translation="MPDRLSWAEIRRLALHHKKALILANLVAVLATLCSVPIPLLLPL
                     LVDEVLLNDGDAALKVMDNFLPAHWETAAGYIGLMLVLTLFLRGGALIFNVLQARLFA
                     RLSKDIVYRIRIRLIERLKRISLGEYESLGGGTVTTHLVTDLDTLDKFVGETLSRFLV
                     AVLTLAGTSAILIWMHWQLALLILLFNPLVIYATVQLGKRVKHLKKLENDSTSRFTQA
                     LTETLEAIQEIRAGNRQGFFLGRLGGRAKEVRDYAVASQWKTDASNRASGLLFQFGID
                     VFRAAAMLTVLFSDLSIGQMLAVFSYLWFMIGPVEQLLSLQYAFYAAGGALTRINELL
                     ARKDEPQYPGRVDPFKGRDTVGIEVSGLTFAYNDEPVLNQLNLSIAPGEKVAIVGASG
                     GGKSTLVQLLLGLYTPQAGTIRFGGSSQEEIGLEGVREHVAVVLQHPALFNDTVRANL
                     CMGRERGDDACWRALEIAQLADTIRGLPQGLDSIVGRSGVRLSGGQRQRLAIARMVLA
                     EPKVVILDEATSALDAATEYALHQALNQFLHGRTTLIIAHRLSAVKQADRVLVFDGGS
                     IAEDGGHQELIAEGGLYAKLYGHLQH"
     gene            48867..49964
                     /gene="hppD"
                     /locus_tag="VFF22_10505"
     CDS             48867..49964
                     /gene="hppD"
                     /locus_tag="VFF22_10505"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HZX17281.1"
                     /translation="MNAVNKIEQHNPIGTDGFEFVEYTAPTPEGIQQLRELFTAMGFT
                     ETAKHRSKEVWLFQQNDINFVLNGSPTGHVREFGLKHGPSACAMAFRVQNAAQAAAYV
                     ESQGAKLVGSHANFGELNIPCVEGIGGSLLYLVDRYGDKSIYDVDFEYIEGRTPNDNS
                     VGLKELDHLTHNVKRGQMDVWSGFYERIANFREIRYFDIEGKLTGLLSRAMTAPCGKI
                     RIPINESSDDKSQIEEFIREYQGEGIQHIALSTEDIYATVRQLRANGVDFMVTPGTYY
                     DKVDTRVAGHGEPSDVLRELNILIDGAPGVGTSGDDGILLQIFTNTVIGPIFFEIIQR
                     KGNQGFGEGNFKALFESIEEDQIKRGVISEE"
     gene            49969..51108
                     /locus_tag="VFF22_10510"
     CDS             49969..51108
                     /locus_tag="VFF22_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020306628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HZX17282.1"
                     /translation="MSRNWISFPLREGECSRQAHCDFPEGTYEREMGREGFFGPTAHL
                     HHKHPPTGWIDWQGPLRPHAFNFNDISSERDCPLAAPLTLHNSDVKLRVWKTHGAMRH
                     LVRNADGDDLLFIHEGAGHFYCDFGHLTYRDGDYLLIPRGTAWRIEASEPTFMLLIES
                     TDGAYQLPDKGLLGPQAIFDPAVLDHPRIDDAFKAQQDENTWQIRIKRRAQISTVTYP
                     YNPLDVVGWHGDNTVVRLNWRDIRPLMSHRYHLPPSAHTTFVANGFVICTFTPRPVES
                     DPGALKVPFYHNNDDYDEVLFYHRGNFFSRDNIEQGMVTLHPCGFPHGPHPKALKKAQ
                     TDPATFIDEVAVMIDTRRALEVGEAAAGVDVPEYVNSWRAPGKES"
     gene            51108..52091
                     /locus_tag="VFF22_10515"
     CDS             51108..52091
                     /locus_tag="VFF22_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZX17283.1"
                     /translation="MKLASLNQGRDGVLVVVSRDLSRATEVPAIAVTLQGALDNWAVA
                     KPKLEAVYQRLNDGLEEGAFAFDQAACHSPLPRAYHWADGSAYVNHVELVRKARGAEI
                     PESFWHDPLMYQGGADCFIPPHSPIQMADEAWGIDLEAEIAVITDDVPMGATAAEAAS
                     HIQLLMLVNDVSLRNLIPGELAKGFGFYQSKPSSSFSPVAITPDELGDTWRDGKVHRP
                     LVSHINGALFGQPDAGVDMTFNFPTLVAHAAKTRPLGCGTIIGSGTVSNYDRSAGSSC
                     LAEKRMLEIIESGEAKTPFLKFGDRVRIEMFDAAGHSLFGAIDQAVERYEH"
     gene            52081..>52439
                     /locus_tag="VFF22_10520"
     CDS             52081..>52439
                     /locus_tag="VFF22_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014595829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HZX17284.1"
                     /translation="MSTELTLYGYWRSSAVYRVRIALNLKGLAYQQVPVHLVKDGGQQ
                     HSAEYQALNPQELLPLLVDKGNGGVRIAQSLAILEYIDEVFPAQALLPSNPAQRAQVR
                     ALALHIACDLHPLNNLRV"
BASE COUNT         9468 a        15605 c        16900 g        10466 t
ORIGIN      
        1 caacaaagga ggaagccctg tggctgatgc aatccgtttg ccgcttgagg tgttttatga
       61 gcgcgaggct cggcacccga ataaacgcta catggtgcag ccgctgccgg gcggtcagct
      121 gcaggaactg agctgggccg acgtcggcga gcaggcgcgg cgggcggcca actggctgcg
      181 tagtcgcgaa ctgccgcagg gcagccgcat cgcgatcatt tccaagaact gcgcgcactg
      241 gatcgttacc gatctggcgg tctggatggc cgggcacgtc tcggtgccgc tctatcccaa
      301 tctcactgcc gagtctgtcg gccaggtgct gcgccactct gaggccgcgc tggtgttcgt
      361 cggtaagctg gacgattggc cggccatggc ccccggtgtg ccgccggatg tgccgaccat
      421 cggcctgccg ctgcgcccgc agggcgagtt cagctttacc tgggatgatc tgcaggcttg
      481 cacgccgatt caggacaacc ccaaggccgc agccgatcag ctcgccacca tcatctacac
      541 ctccggtacc accggcacgc cgaaaggcgt aatgcatcat ttcagcaatt tcggctttgc
      601 cgccagccac gccaccgagc tgtttggtgt tggcgaaaat gaccgggtgc tgtcctacct
      661 gccgctgtgc catgtggccg agcgtatgtt tgtcgagctg gcttcgatct acgccgggca
      721 gacgatcttc ttcgccgaaa gcctggatac cttccttgaa gacctcaaac gcgcgcggcc
      781 aaccgtgatg ttcggcgtac cgcgtatctg gaccaagttc cagatgggcg tctacagcaa
      841 gatgccggcc gagaagttgg atttcctgct caagctgccg atcatcggcc gcatcgttgg
      901 caaaaaggtg ctggtcggcc tgggcctgga tgctgtgcgt tatgcgctgt ctggcgcggc
      961 gccggtgcct gaagcgctgc tcaactggta caagcgcctg ggcctggatg tgctggaggt
     1021 atacggcatg accgagaact gcggttactc gcatgtgtgc cggccgggta agttcaaaca
     1081 gggctggatc ggccagaaca gccctggcgt ggaagtgcgc atcgccgagg atggcgaagt
     1141 gcaggtgcgc agcggcgcga ccatgcaggg ctactacaaa gatccggaaa agactgcgga
     1201 aaccatcacc ccagatggct tcctgcgcac cggcgataag ggtgagcagg atgccgaagg
     1261 caacctgcgc ctgaccggac ggatcaagga aatcttcaaa accagcaaag gcaaatacgt
     1321 cgccccggcg ccgattgaaa accgcctagg cgagcattcg cgcatcgagc aagtgtgtgt
     1381 ggtcggcgat ggcatggccc agccgatggc gctgtgtgtg ctgtctgacg tgggccgcag
     1441 cgaggccctc aacggcgcgc gtggcgagct ggaaaacagc ctgaaaaagc tgttggagga
     1501 ggtcaatcaa cgcctcgaca agcatgagcg cctgcagcgc ctggtgctgg tcaaggaggt
     1561 gtgggcggtg gataacggct tcctcacgcc taccctgaag atcaagcgcg cggtggttga
     1621 aggcacctat ggtgaacgct tcgacgagtg gaccactcgc agcgaaaccg tgctctggca
     1681 cgagtgatca ggcccatggc cggcatgcag tgtaccggcc atgttctggc tgaatcgacc
     1741 ttaaaggact gactatggcc ctctggcagc aaaccccgaa tctcgaacag ctcaacgcga
     1801 ttctgaaaaa taccatcggc gagcaactgg atatccgctt cgagtcgttc gatgacgagt
     1861 cgattaccgc cagcatggtg gtcgatgcgc gcacgcatca gccctacggc ctgctgcatg
     1921 gcggcgcgtc ggtggtactg gcggaaaccc tcggttccac tgccagtcat ctgtgcatcg
     1981 acagcagcaa gttctactgc gtcggcctgg aagtgaacgc caaccacctg cgcggtttgc
     2041 gcagcggccg ggttacagcg gtcgcccgcg ccgtgcatct ggggcgcacc acccatgtct
     2101 gggatatccg cctgagtggc gcggatggca aggccagctg catttcccgg ttgaccatgg
     2161 ccattgtgcc gctgggtgag aacggcccgg cgctgcgtta gaacctgtcc aaagtctgct
     2221 gcgcgtcggc cctgctgcgt taaaaacagg ctcggaatgc tcatgtacaa aagtacactc
     2281 cgcttcctcg cctgtttttg ccttgcatgg ctctagctcg cgagactttg cacagattct
     2341 tagtgctacc aaaccaataa cttgtcagcg tcgagtcaac tggcgggcct cccatcagct
     2401 ttggctatag tgccgtcatt cactggccgt tttgtcattg ctgcatcgct ggtgctgccg
     2461 gacaatcatt ggcaactttc cagataagcc cgccgccatg acccagtccg tgttcttcgc
     2521 ccatgccaac ggctttccct cggccactta cggcaagctg tttgccgcgc tggcgcctga
     2581 ctattcggtg cagcacctag agcagcacgg ccatgacccg cgttttccgg taaatgacaa
     2641 ctggagcaac ctggtcgacg aactgatcca tcacctcgaa gccagcccgc agccggtatg
     2701 gggtgtgggt cattcgctgg gcggtgtgct gcattaccac gccgccttgc tgcgccccga
     2761 gctgtacctg ggcgtggtga tgcttgattc gccagtgctg accctggccg acaagatggt
     2821 tattcgcgcg gccaaacgct ttggctttat cgaccgtatc accccggccg ggcgcaccct
     2881 gggccggcgt gaggagtttg tcgacctggc cgaggcgcgt ggctatttcg ccggtaaagg
     2941 cttgttccgc cgcttcgatc ccgaatgcct ggacgcctat gtgcaacatg gcctgcaagc
     3001 ggcgggcaat aacctgcgtc tgcgttttga tccggccacg gaaatcagta tctaccgcag
     3061 cgtgccgcac accagcccgg gccggccgca acaattgaag gtgccgctgg ccatggttcg
     3121 cggccgccat agccgggtag ttatgccgca tcacgcccat ctggtgcggc gcgtgccgaa
     3181 gggcgagtac ctgaccctgc ccggcggcca tatgtttccc ctggaacgcc cgcaggacac
     3241 cgctgagctg ctgaaaaagc tgttccagcg ctgggccgga caacctgcaa gcgaacaaag
     3301 tgcatgagcc tgaatttcga agaagtgcgt ctgcgcctgc cgcatatcga gctggccgct
     3361 cacctgtatg gccccgaaga cggcatgccg gtgattgccc tgcatggctg gctggacaat
     3421 gccgccagct ttgcccgcct ggcgccactg ctgccggggt tgcgtattgt ggccctggat
     3481 tttgccggcc acggtcattc cgatcaccgc ccgccaggcg gcagttacgc catctgggac
     3541 tatgcccacg atgtgctgca ggttgccgag cagttcggct ggcagcgttt ttccctgctt
     3601 gggcattcca tgggcgcgat tgtttcggta ctgctggctg cggcgatgcc ggagcgtatc
     3661 gagcgcctgg cgctgatcga cgggctgatt ccctacacct gcgagccgga cacggcgccg
     3721 cagaaactcg gcgaggcgct caaggcgcag ctggccctgg ccggcaaaag caagccggta
     3781 tatgcggagt ttgcccgcgc cgtggaagcg cggatgcgcg gagtcggcgc agtcagccgt
     3841 gaagctgcag agttgctcgc tcagcgcggc ttgatgccgg tgcccggtgg ctacacctgg
     3901 cgcaccgaca gccgcttaac gctgccgtcg ccactgcgcc tgactcatgc tcatgcgctg
     3961 gcgtttgtgc gcaacctgca atgcccggcc aagctggtga tggcgcagca gggcatgctg
     4021 ctcgcacagc cgagcattac tcagctggtc gagggcctgg ccatcgaggt ggcgcagctg
     4081 cctggcggcc atcatctgca cctagatgac gacgtgggcg cgggcttggt agcagactgt
     4141 ttcaaagctt tcctggccgc gccttgactt gctaaaggca actggggaaa ggtgtcggcg
     4201 atacccacgg agattcgcat gatcgacctg tataccgccg ccacccccaa cggccataaa
     4261 gcgtccatcg cactggaaga gttacagctg ccttacaccg tgcaggcttt gagtttcgac
     4321 aagcaggaac agaaggctgc ggccttcctc aagatcaacc ccaatggccg tattccggcg
     4381 attgtcgacc gcgaaaatga taattttgcc gtgtttgaat ccggcgccat tctgctgtat
     4441 ctcgccgaga aaaccggcca actgctgccc agcgacgcca aaggccgctc gcgggtgatc
     4501 cagtggctga tgttccagat gggcggggtc ggcccgatgc agggccaggc caatgtattt
     4561 ttccgctatt tcccggaaaa gctgcagggt ccgatcgacc gttatcagca cgaaacccgc
     4621 cgtctttatg aagtgctcga cacgcgcctg ggcgagtcgc agtacctggc cgatgactac
     4681 agcatcgccg atatcgccac ctatccctgg gtacgcattc atgactgggc cggcgtctcc
     4741 gtcgacggcc tggaacatct gcagcgctgg atggctgagc tggccgcgcg ccctgcagtg
     4801 caacgcggtt tgctggtgcc gcagcgcgaa gcagacggcg aaaaagttgt taaagcggcg
     4861 caagcgatgt tgatcaaatg agccgcctta atcgttattg cctcggtctg agtctgctgc
     4921 tgagcagcgc cgttgtctgg gcagatgtgg ccggcagtca ggacctggac gtgttgccgc
     4981 gctttgccgg gagcaagatt gtcagcttca aggatgttgc cgaacaggag cgtatttacc
     5041 cgcaaggggc gattcggcgg atcagcggca cattgcgcta cgagcgcgaa gtggccgccc
     5101 agggccagtt gacctccgtg acttatgagc tgccgcgcac ccacacggcc aatgaggtgt
     5161 tcgccaaggc ccgcggcgat ctgcaagccc aggacgccga gttgctttac tggtgtgtgg
     5221 gccgtgagtg cggttcaagc agcctgtggg ccaatgcggt atttaccaat gccactctga
     5281 ccggctccga tgaccagcag acctacgcat tgctgcgtct ggccgaaccg cgccaggaca
     5341 gcctgttggc gctctatagc attacccgtg gcaacaagcg cgcttacctg catgccgagt
     5401 tgctgacggc caatgcgcca ttgggtgacg tgctgcccac cccagccacc ctgcagcggc
     5461 agctgaaaag caccggcgaa ctgcgcttgg ccacgcaggt tgaacccagc gctgcctggg
     5521 ttgaactgct cgcacgtagc ctcaaactcg acagcaccct gcgcgttatc atcagcggcg
     5581 cgcaagccga agcctggcgg gaggcgctgg ttgaacagcg tgtgcgcgcc agccgtctgg
     5641 agctgggcga ggcggatgtg aacggcgtac aactcaacgt gctgcgctaa gacgccactg
     5701 tcggtactct tgtcgttatt aattgatccg gctctgtccc tcgtggcaga gcctttttgc
     5761 gttgtgcagg gagctggttg aatggccaat aacgatcgtt tgctggtgca gatactgctg
     5821 ctggttttgt tgggtgccag cctgtgggtg ttggcaccgt tctggtcggc gctgttctgg
     5881 gctgcggtgt tgtcgtttgc cagctggccg gtgatgcgtg tgctgacgcg cctgctcggc
     5941 ggccgtcagg cgctggccgc aggtctgctg accctggcct ggactgtagt ggtggcagtg
     6001 ccgttggtga tgctcggctt caacctggcg gatcacatca aggatgtcac cgcgctgctc
     6061 aaggatttcc aggtcgatgg cttgccgccg gcacccacct ggctggccgg cgtaccgctg
     6121 gttggcgagc gcctggtggg tttctggatg accatcgacc agcagggcgc ggcgttcttt
     6181 gccaccgtgc ggccgtactt ggggctggtc ggtaactggc tgctggcgcg cagtgcgcag
     6241 atcggtggcg gtatgctgga gctggcgttg agcctgattc tggtgttctt tttttaccgt
     6301 gatgggccgc gtctggcggc gtttgccgaa agcctgctgc aacgtttgat cggtgatcgc
     6361 gccgagcatt atctggagtt ggtggccggc actgtgcagc gggtggtcaa cggggtgatc
     6421 ggtactgccg cggcgcaggc gattctggcc tggattggtt tcaccatcgc cggtgtgccg
     6481 ggtgcgctgg tactggggat tctgaccttc ggcttcagct tgatcatggt gccgccgctg
     6541 atctggggac cagccgtggc ctggctgttc tggcagggcg aagtcggcat ggggttcttc
     6601 ctgctggtct ggggcttctt tgtgatcagc ggcgtcgaca acatcctcaa gccgtatctg
     6661 atcagccgtg gcggcaactt gccgttgatc gtggtgctgc tcggggtatt tggcggcatt
     6721 ttggcgtttg gttttatggg gctattcctc ggcccaaccc tgttggccgt ggcgtacagc
     6781 ctgctcagtg actgggttgg tagcccagtt gtaaacaccg ctgcgagtag cgccgagcca
     6841 acagacaagc cgctcaactg agttcgcagt gccaccgtgc tggtgttcga gccggttggc
     6901 attctcggca aatttccccg gcggcttctt ggtcgcctgt ggccgcagtg gtagccttgc
     6961 tgcgcttcgc ggcgcctatc ggctgccgca gccaggcgtt ggtcgcaagc ccaacgccgc
     7021 gccttaggcc gcaagtatgc ccagcagtgg caggcggcag cagggaagac cccacacacg
     7081 taacggacac gcaggattaa ccatgaagaa aattgcaggc agcgcccttg gcgcagttgt
     7141 cgttctcggc gcattgagca ccgcaggtgc ctggtacacc ggccagcagc ttccagcggt
     7201 gctggaaacc tcgattcagc aagccaacca ggatatgggc aaaaccctgc cggccttggg
     7261 cctcagcgcc accatcgagc tggtttccct ggagcgccag ctgttcagca gtaacgcgcg
     7321 ctaccgcatt cagtttgccg gcagcctgga tggcgaatcg ccgagcaact tcgagtggtt
     7381 cgtcaccgac cgcatcgagc atgggccatt cccgctttcg cgactgcaaa ccctcaagct
     7441 gctgcccgtt atggccacca gcaactatga gctggaacgc agcccggagc tggagaagtg
     7501 gttcgccggc agtcagggcg gtgcgccgct gcagggccag gtcagcctcg gttatgaccg
     7561 ttcgctgagc ggcaacctgc gtgtgcaacc gctgcaagtg gcgctggatg agcagaccaa
     7621 catcgatttc agcggcctgg aaatcgactt cgacagcagc gccaatgtcg agaaaatcag
     7681 cgccagcggc gtgatggaca gcttcaagct cacctcaacc ctgtctaccg atgaaagcat
     7741 gcgccttgag ttcaagggca tggacctcac cagtaacacc cgcaagggcg tcagcgattt
     7801 ctacctgggg cagaacgagg tgcgtctgca acagatcgag ctgcagattg gcgagagcgc
     7861 acccatcctg ttcaaggatt tcgtccagcg cgatgaaacc agcgaagcca accagcagct
     7921 gtcggcgcgt tacagctatg acgtcggcat gatcagctac cagggcaacg acataggttc
     7981 ctcgcagatg ctctggaccg tgaagaacct cgacgcggcg gctttacagt cgatgatcga
     8041 gctgtacggc acgctgctgc agagcggcaa ggtcgacagt gaaagcggta tgccggaact
     8101 gaccgacgag cagactgcgc tgttcaaaac tgatctggaa aaactgctgg ccggcaaacc
     8161 gggcctggcc ctggaaaaac tcgcgttcaa gaccgccaac ggcgagagca gcttcagctt
     8221 tggcttggac ctgaacaagc cggtgtcctt cgacttgccg gcgcctgaac tggccaagca
     8281 gttgatcgct cagttggatg ccaaactgct ggtgtccaag gcgatgatta ctgatgtgat
     8341 tggcgtgcag gcgaccctgg ccggggaaac cgacaaggaa gcgattgctc agcaggctgc
     8401 gatggtgggt gaaatggcca gcggtatggc ggtggccagt gagctggccg tgctgcaggg
     8461 cgaggacatc agctccagcc tgcattacgc caacgaccaa gtgaccttca acggcaagca
     8521 gatgaccgtg gaccagtttg tggcgttggc catgagcagc ggcggcggtc tggctggcat
     8581 gggccatgaa accccggccg atgaagtgct gctggacgaa agcgagggtg aaagcaccat
     8641 tcagtaaagc cttcagcgct acgccaccgg cgtagcctgc gcggtaaata cggccagacg
     8701 acctgtaaag cagggcgcct ggccgttttt gtttgcccat gacccgcgat ctttacgctt
     8761 tctttatgcc gggcggcatc gcccgcactc gttcctttac gccctggttg ccgacaattt
     8821 gccccaagcg gtagccaccg taacaaggag caaacacatg agcattctcg acggggtgtc
     8881 cctaggcctg gccttgggac ttttcatcta tttgttggtt gcgctactgc gcgctgatca
     8941 ggcgtagggg cgcatatgca cagttacgat ttcactttga tcctggcgtt cttcgtcctg
     9001 gttctgctgc cggcgccctt tatcgggcgt tacctgtacc gcgtgatgga gggcgaaaaa
     9061 acctggctca gccccgtgct gcagccggtc gagcggctgt gctaccggat cgccggtgtc
     9121 gatagcaacc gcgagcagga ctggaaaacc tacagcctgg cgttgctggc cttcaccctg
     9181 atcagccttc tgacgctgtt cgccatcctg atgctgcaag gcagtttgcc gctcaacccg
     9241 cagcaattgc cggggttgga gtggacgctg gcgttcaaca ccgccgtgag ttttgtcacc
     9301 aataccaact ggcaggcgta tagcggcgaa gcgcagatga gctatttcag ccagatggtt
     9361 ggcctgggcg tgcagaactt cgtcagcccg gctgtcggtc tggccgtgct ggtggtgttc
     9421 tgccgcggca tcgcccggcg ttcgagcaat agcgtcggca acttctgggt cgatctgacc
     9481 cgcgccacgc tctacggcct gctgccgttc tgcctggttc tggcgctgct tctggtgtgg
     9541 cagggcgtgc cacaaacctt cctcgaatac gtcactgcgc acaccatcgg cggcaccgat
     9601 caggttgtgc cacttgggcc tgcagccagc cagattgcca tcaagcagtt gggcaccaac
     9661 ggcggcggtt tcttcggcgt caactcggcg catccgttcg agaacccaac ggcctggagc
     9721 aatctattcg aggtggcctc gatcatcctg attcctgctg cgctgctgtt caccttcggc
     9781 cattacgtca aagacctgcg ccagagccgt gcgttgctgg cctgcatgct gattctgttc
     9841 agtgtcggcc tgggtggcac cctgtatgcc gagcatcagc cgaacccggc gctggccgca
     9901 ttgccgattg agcagagtgg ttcgctggaa ggcaaggaaa gtcgcttcgg caccacggcc
     9961 tcggcgttgt gggcggtgac caccacggcc gcgtccaacg gctcggtcaa cgccatgcac
    10021 gacagcttca gcgcgctggg cgggatgatc ccgctgttca acatgatgct cggcgaggtg
    10081 atcttcggcg gtgtcggtgc tggtctctac ggcatgctgc tgtttgtgct gatcgcggta
    10141 tttctcgccg gcctgatgat cggtcgcacc ccggaatacc tcggcaagaa gctcgaagcg
    10201 cgcgaagtac gcctgctggt ggccaccttg ttggtgatgc cgattggtgt gctggtattc
    10261 ggcgccctag cggccagcct gcctggcccg gcggcctcgg tcaccaaccc aggcgcacac
    10321 ggctttagcc agattctcta tgcctacacc tcgggtaccg ccaacaacgg ttcggccttt
    10381 ggcggctttg gtgccaacac gccgtatcac aatctgatga ttggtctggc catgttgctc
    10441 ggtcgtttcg gctacatcct gccgattctg gcgattgccg gcagcctggc ggcgaagaaa
    10501 cgcgcgccgg tgggcagcaa cagcttcccg acccacggcc cgctgtttgt caccttgctg
    10561 accctgacca ttctgctggt cggcggtctg accttcctgc ccgcactggc cctcggcccg
    10621 attgctgagc atatgactct gtttcagggc ttctaaggga gaaccatgat gaatgcccaa
    10681 actcaagtaa aaagcccggc gctgaacagc gcccagcaac acccgaaaac ctcgctcgct
    10741 gcattgtgga agccggcgct gcgccaggcc ttcgtcaagc ttgatccacg ccagctgccg
    10801 cgctcaccgg tgatgctggt ggtcgagctg actgccgtgg tcaccaccgt gctgtgcttt
    10861 atccccaacc ctcaggtctc taccggcctg gccgtgcaga tcgctctgtg gctgtggttt
    10921 accgtgctgt ttgccaactt cgccgaagcg cttgctgaag gccgtggcaa agctcgcgct
    10981 gacagcctca aggccggcag ccaggggctg aatgcgcggc gcaagaatgg tcagcagttc
    11041 gagaccgtgg cggccagcag cctgcgccgg ggcgatatcg tgcgggtcga ggccggcgaa
    11101 ctgatccctg gtgacggcga agtcgtcgaa ggcatcgccg cggtcaacga ggcggcgatt
    11161 accggcgaat ccgcgccggt tattcgtgag tccggcggcg accgttcggc cgtcaccggc
    11221 aacacccgag tagtctccga ctggttgctg gtacagatca ccgccaaccc cggcgagtcg
    11281 actctggacc ggatgatcgc cctggttgaa ggcgccaagc ggcagaagac gcctaacgaa
    11341 gtggcgctgg atattctgct gatcggcctg accctgatct tcctgctggt ggtggcgacg
    11401 ctgcagccgt tcgctcgttt tgccgagggc gacctgccgc tggtgtacct ggtggcgctg
    11461 ctggtgacgc tgattccgac caccatcggc ggcttgctct cggccatcgg catcgccggc
    11521 atggaccgct tggtgcgcct gaacgtgatc gccaagtccg gccgcgcggt ggaagcggcg
    11581 ggggacgtgc atgtgctgct gctcgacaag accggcacca tcacctttgg taaccgccgt
    11641 tgcagcgcgc tgtacaaggc gccggggatc tccggcaagc agcttggcga tgcggcgttg
    11701 ctggcttcgc tggcggacga caccgcagaa ggcaagtcga ttgtcgaata cctgcgcgcg
    11761 ctgaatacca tggttgagcc tgagcgcagt gcgatcaaat cgattgcctt cagtgccgaa
    11821 acgcgcctgt ccggcgccga ctacaacggc cacagctacc gcaagggtgc ggtggatgcg
    11881 gtgctgcttt atttaggtct gcagcgcagc gacgtaccgc cggtattggc ccgtgaaatc
    11941 gagataatcg cccagagcgg cggtactccc ttgctggtgg ccggtgatgg ccagttgctc
    12001 ggcgctattc acctcaagga cgtggtcaag ccgggtattc gcgaacgttt tgccgagctg
    12061 cgtgccatgg gcatccgcac cgtgatggtg accggcgaca acccgctgac cgccgccgcc
    12121 atcgccgccg aagcgggcgt cgatgacgtg attgctgaag ccaccccgga gaagaagctg
    12181 cagcgcatcc gcagtgagca ggccgaaggc aaaatggtcg ccatgtgcgg cgacggtgcc
    12241 aacgacgccc cggccttggc ccaggccgat gtgggcctgg cgatgaacga tggcactcag
    12301 gcggcgcgtg aggcggcgaa tctggtcgac ttggacagtg atccgaccaa gctgctcgac
    12361 gtggtgcagg tgggcaagga gctgctggtg acccgcggcg cgctgaccac cttctcggtg
    12421 gccaacgatg tggccaagta cttcgccatc cttccggcgc tgttcgccgg catctacccg
    12481 cagctcggcg cactcaacct gatgcagctg cacagcccgc agagcgcgat tctttcggcc
    12541 atcgtgttca acgcgctgat catcgtcgcg ctgattccgc tggccctgcg cggggtgcgt
    12601 gtacaggccg ccgatgccgc cagcctgcta cgacgcaacc tgctgatcta tggcctgggc
    12661 ggcctgctgg cgccctttgt cgggatcaag ctgattgatc tgttgttggt ggctgctggc
    12721 ctcgtttaac caagcgcaat ccgtagcccg gatgaaatcc gggagatccc atccccgggt
    12781 tgcagccggg ctacacaaaa actggagagt catcatgttt aagcaaatcc gcccagccat
    12841 tagtgtgctg gcgtttatga ccctggtcac cggtgtggtt tatcccctga ccgttactgg
    12901 ggtggcgcag ctggcgttcc cggcgcaggc caatggcagc ctgttgcaca atcaacaagg
    12961 cgacgtgctc ggtagcgcgt tattggcgca acccttcgag ggcgcgcaat ggtttcagcc
    13021 gcgcctttca gccggtggtt acgccaccgt ggccagtggt gcgagcaacc tggcaccgag
    13081 caacccggcg ttggctgagc gtatcgctac agatgcccag cgcttgcagg ttgaagacca
    13141 gggcgcagta cccatgcagc tggtgaccac ctcggccagt ggcctcgatc cgcatctgtc
    13201 gccggcagcg gcgcagtttc agatcgcccg aatcgccgca gcacgtggca tcccggcggt
    13261 cagcctggaa cgactgatcg agcagcacgt ggaaaagcca ctggtagggc cggcagtggt
    13321 caatgtgctg gcgctgaacc tggcgctggc cgacaattcg cctcccgcaa aagctgagta
    13381 acctgcatga gtgatgccgt acgcgccgac gccctgctcg atgagatgcc gcgagaaggg
    13441 cgtggccggc taaaggtctt tctcggcgcg gcgcctggcg taggtaagac ctacgccatg
    13501 ttgcaagcgg cgcaggcgca attgcgccag ggcgtcgacc tgcgcgttgg tgtggtggaa
    13561 acccacggtc gtgcggaaac cgaggcgatg cttaccggct tgccgcaaca gccgcagcag
    13621 cgtctggaat accgtggcgt ggcgctgagc gagatggacc tcgacggcat tctggccaag
    13681 ccgccaaagc tgctgctggt tgatgagctg gcgcacagca atgcccccgg cagccgccac
    13741 agcaaacgtt ggcaggatgt gcaggagctg ctggatgccg gcatcgacgt gtacaccacg
    13801 gtcaacgtgc agcacctcga agcactgaat gatcaggtgc gcgatatcac tggcgtgcag
    13861 gtgcgcgaaa ccctgccgga ctgggtgttg caggaagccg acgagattct gctgatcgac
    13921 ctgccgccgc gtgagctgct ggagcgcctg cgtgaaggca aggtgtatgt accggaacag
    13981 gcgcgcgccg ccatcgatgc gtttttcagc cagaccaacc tcaccgcgtt gcgtgaactg
    14041 gcgatgcaga ctgctgctgc gcgggtcgat gcggatctga gtcggcgcta ccgccagcaa
    14101 ggtcagcaag cgcctgcgct acgtggccgc ttgctggtcg gtattgacgg cgatgcccaa
    14161 gctgagcgcc tggtgcggca tgcctgtcgg gtggccgaac gccgtcatct gccctggtcg
    14221 gtggtgtatg tggacaccgg cggtgagcgc gatgaaaccc gtttgagcta cctacagggc
    14281 gcgcagcaac tggccgaacg cctgggcggt gaggtggtca gcctgcgcgg tagcgaggtg
    14341 gcgaaaatcc tgatcgagca tgcccgcgaa cgccgcgcca gcctgattct ggtgggccgc
    14401 agtcgacaac gtctacggcg tatctggcac ctcggtcgtg gcctggcgga gcgcctgttg
    14461 cagcaagggc agggcctgga aatcagcgtg ctggataccg aggcgagcaa tgccccggca
    14521 ctgtcgcggc ggcgtcagcc ggtggtctgg cgtgactatc tgctggcgct agtagctact
    14581 ctgctggcct cggtcgtggc gctgggctta tcccgtgtac tggaactgcc gaatatctcg
    14641 ctgattctgc ttgcggcggt cttggtggtg gcggtgcgca gtagcctggg cccagccatg
    14701 gcgtgcgccg ggctgtcgtt tctggcgtac aactttctgt ttatcccgcc gaccttgacc
    14761 ctcaccatcg accgtcagca ggacgtgctg accctgctgt tctttctgct gatggctggc
    14821 ctcaccggta acctggccag ccgtcagcgt cgacagttgc aggccctgcg tgagacgcag
    14881 cgagaaacca gcgcactact ggagttgtca cgcaagctga cggcggccac cgaccgccag
    14941 gcagtgctgg cggcggccat gcagcagctg ggcctgtggc aggatgtcga ggtcagcctg
    15001 ctgtcgcgta gcccggatgc actctggaag gtggaggcgg gggcgcagcg cctgctcact
    15061 gatctggaac gagcggcggc ggactggtcc tggcagcacg atcagccggc cgggttgggt
    15121 actggcacct tgccggatgg gcgctggtgg tggttgccgt tgtcgggcga agaggggccg
    15181 ttgttgttgc tcggggtcag ccccaaggac ggcagggcct taccggccga acgacggcgc
    15241 ctgcttacgg cgttgggcca gcccctagcc caggccctgg agcgcgcaca gctggccgaa
    15301 gacttggaag ccgcacgtct gcacgggcaa accgaagagc tgcgcagcgc gctgctggcg
    15361 tcggtgtccc atgatctgcg cacgccgctg accgccatgc gcggctcgat cgacagcctg
    15421 ctggcacttg gtgagcaaat tcccctggct gatcgccgcg aactgctgga gggcacccgc
    15481 gatgaggccg agcgcctcga ccgttatatc cagaacctgc tggatatgac ccgcttgggc
    15541 cacggtggac tgaagctggc gcgtgactgg gtggcgccgg cggatatcgt tgccagtgcg
    15601 acccagcgct tacgcccggt attgtccgcc ttgaaactgg agatacaggt gccgcaggag
    15661 ctgccgctgc tctatgtgca tgccgcgctg atcgaacagg cgctggtcaa tgtgctggag
    15721 aacgccgcgc ggttttcccc gcctcagggc cgtttgcgtg tggccatcga ggcggataca
    15781 gcagaactgc gctttattgt cagtgatcag gggccgggta ttcccgagca tgagcgcacg
    15841 aagattttcg atatgttcta caccgccgcc cggggggatc gcggcgggca gggcactggt
    15901 ctgggcctgg cgatctgcca gggcatgctc ggcgcccatg gcggccgagt gacggtgggc
    15961 gaagggctgg atggcctggg agcaagcctg accctgcacc tgccgttgca cacgcagccg
    16021 cagatggaag aagaacagtg atggatagcc gatgagccaa aaccccgcaa ccattctggt
    16081 tatcgatgac gaagcgcaga ttcgcaaatt cctgcgcatc agccttacgg cccagggcta
    16141 caaagtgctg gaaggcgcta ctgggcagga aggtctggcc caggccgcgc tgggcaaacc
    16201 ggacctggtg gtgctcgacc tcggcctgcc ggatatggat ggccagcagg tgctgcgcga
    16261 gctgcgcgcc tggtcgcagg tgccggtact agtgctgtcg gtgcgcgcca gcgagggcga
    16321 aaaggtcctg gccctggatg gcggcgccaa tgattacgtg accaaaccgt ttggcattca
    16381 ggagttcctc gcccgcgtgc gcgtgttgct gcgcctggcc ggcggtgtgg aacaagccag
    16441 tgcggcagtc agcagcggcc cgctgcaggt ggatttcgcc taccgcaggg ttagcctcga
    16501 tggtgttgaa gtcagcctga cccgtaagga atacgcggtg ctggcgcaac tggcgcggca
    16561 tatcggccgg gtggtgaccc agcagcaact gctcaaagac atctggggcc cgacccatgg
    16621 cgaggacagc cactacctgc gcgtggtggt cggccaccta cggcagaaac tcggcgatga
    16681 ccccaccgcg ccgcgcttta tcgtcaccga ggcaggtgtt ggctatcgcc tgcgtgattc
    16741 cgcctaggtt ctagagctta tttacgatct cgcgagctag agtcctgcaa ggcaaaaaca
    16801 ggcgaggaag cggagtgtac ttttgtacat gagcatttcg agcctgtttt taacgcagca
    16861 gggccgacgc gcagcagatc gtaaaaagtc tctagcggtc gccgcgctcg tacttatcga
    16921 gggtatcgct ggcaatctgc cggccgagca tgatcagctc cggcgccttg tagaactcga
    16981 agaagcggca tacccgcttg ggtacgttga tcaggatatc cggcgggtag ccggcgatct
    17041 tgtattgcga caacgaggtc tgcattactt cgaagctctg gttgatcagc tccagtagcg
    17101 aggcggggcc gctgaggtcg gctacacgcg agccactggc ggatttcggc gcggccggtt
    17161 tgtccaggct gccacgggta ttctgctgcc aggggttgat ggcttcgggc tgcattacat
    17221 ggttctggtc ttctagcaga agcaatccgt cgtcttcgcc gggtttgcgt ttgaatgagg
    17281 gcaggcgcga accgagcgaa ctcatcaaca gatcgaagcg ccccttgagc gccggcgtac
    17341 gctcgataat cggcagctcg taatgccgct gatgagccga gttgaggttg accgcgacaa
    17401 tcagatcgca gtgcgcggac actaccggga cgatgggcag cgggttgagc aggccgccat
    17461 cgaccagcat gcgtttgccc tgtaccactg gggtaaacag gctgggaatc gccgctgagg
    17521 cgcgcatggc ctggtgcagg cagccttcct gaaaccagat ttcctgctga ttggtcaggt
    17581 cggtggccac cgcggtgaag gggatcgcca ggtcttcaat attgatttcg ccgactattt
    17641 cgcgaatctt gccgaacacc ttttcaccgc gaatcgcccc caaacgaaag ctcacatcga
    17701 gcaggcgcag cacgtccaga taatccagac tttgcgtcca atcgcggtat tcattgagtt
    17761 tgccggcggc gtagatgccg ccgaccaccg cgcccatgga gcagccggca atgcaggcga
    17821 tttcataacc acgggcttcc aactcctcaa tcacgccgat gtgcgcataa ccgcgcgcac
    17881 caccggagcc cagtaccagc gcaatgcgct tactcatcct gatgtccccc atactgttta
    17941 tgcagcttat gcagcgaaca tacgcgccgt gccgcgcgcc gccaaggcgc gatttgctag
    18001 tatccagcgc agtcgagttt acgcgctgtg gcttgctaga gcggttgcag cggtgttggc
    18061 acttttctct gttctacgcg tcatactggc agcattgatg catcgcgaac attgacggcg
    18121 atgtgttcgg atgccgagtc aattgtctgt ctatcttgca ggagttacct gatgaaactg
    18181 tggatcgtgg tgcccatgtt gctgttggtg ctgagcggtt gtgcgggaaa aacggcttat
    18241 cgtgacagct gcgccaatca gttggacgct gcctggcgtg agctggatct ggccaaggct
    18301 gagggctttg ccggtacggt gagttactcc aaggcggtgt cgctgatgac ggcggccaaa
    18361 acccagcagc aattcgaggc ttataagggc tgtacggcga agtccgagcg cgcccgtttc
    18421 tatatccgtg agtcgcgagc aggccgctga tgcaactgga tttcagtgca ctcgaaccgc
    18481 tcgaacgcta ccgctggttg gcatcgacca tcaccccgcg gccgattgcc tgggtttcca
    18541 gcctgtcggc cgaaggggtc agtaacctgg ccccgttcag cttctttcag gtgatcagcg
    18601 atgacccacc gaccctgatg gtcaacgtca atcaccgcgc cgatggcggc ctgaaggaca
    18661 ccttgctgaa tgtgcaggcc agcggcgagc tggtgattca gttggtgtcc tttgcccagg
    18721 ccgaggcgat gaatgccagc gcggcggtgc tgccctacgg catcagcgag tttgagcagt
    18781 gcggcatcgc cagccagcct tcgcagcgag tcgcaacgcc tcgggtggcc ggtgcggcgg
    18841 tggcctttga gtgccaggtg gcgcagattc aggcctaccc ggcagataag cccaattgcc
    18901 atctgatctt tgccgaagtg ctgttggcgc atatcgacga tgcggtgctc aatgagcagg
    18961 gccgtatcga tgcgcacaag ctcgatctgg tcgggcgttt gggcggcatg gcctatagcc
    19021 gtacccgcga tacctttgag atgcgccgcc ctggctgatg cgcgtggctg cctgcggcca
    19081 cgcctagccg cacgaatcgg cgcaaaagcg acctccgttt agctattagc accatgcccc
    19141 agcctgctgg cttgtcgcca gcacggctgt gctcctatgt tgccaatctt gaatgcagac
    19201 agcgcagggc tgtcttgtgg tcattcgggc agggggagct atgtggactg tggtgcagcg
    19261 gcaggtcgcc agtttgagca cggcgagcaa actggccttg ggctttggca ttgttctggc
    19321 gctcaccgcg ctggtggcgg ccaccgggtt tcttgcgctg cgcgatgtca gtgcgcgttc
    19381 gacgctgctg gagcgcatga gcgcgatcaa tagccaggtc atgcagatcc gtcgtagcga
    19441 gcaggatttc gtcctcagca gcgacaagca gcacgccacc cggctacacg aacaggcgca
    19501 gcagattctc gatgccagtg ccgcgctgca ggcccaatta ccgcaagccg agcgcgtggg
    19561 gctggatcag gtggccgaag ccatgacggc ctatcgcgct gctttcgatc gctatgtcga
    19621 gcagagcgac aatatgcatc tgtcgctgga ggccgcgacc tggctggtgg tgagtgcgtc
    19681 caacagtctg gatctgctca aggatggtct ggccgaagac ggcgtcgatc tgctcaagag
    19741 cagccagggc cagcagggcg ccgagtctgt gttgcaggct gggcagatca gtcaggtgca
    19801 tcagctgttg ctgcgtgcgc tggaccaggc ccgcgtgttg ctggaacaaa gccgcagcag
    19861 cggtgaggcg atcccaaccc gcattcagga agccgccgac gcgcaggcgt tggccggtga
    19921 gctacgcgat gccatggagg atcccggcta tgcagcggtg ctggctgagg tgatcggcaa
    19981 cgtcaactcc ttcaatgacc ggctgggcga atacaccggg ttgctgcagc agcaaaaacg
    20041 cgaataccag aacctggtac agcaggccga acgtattctg gccctggttg atcaggccta
    20101 cgcacggcaa aaggctagcc tggaccagca actgcagacc agtacttggc tgatcgtgct
    20161 ggccacggcg ttggcgctgc tgattggcct gctggcgacg ctgaccatca ccctgctgat
    20221 cgtgcgcccg ctcaagcagg tgatcgagca ggcacggcgc attgccgatg gcgacctcag
    20281 tgcacgtatc gaggtgcagc gtggcgatga gatcggccag ttgcaggccg ccatgcaggg
    20341 tatgtccagc aacctgcgcg agatggtcgg gagcctgcag ggcggggtgc tgcagatttc
    20401 cgcgtccgcg cagtcgctgt cggccagtac cgagcaaacg cgtctggggg tgaatgagca
    20461 gaaggcggaa accgatcagg tcgcgacggc catgagcgaa atgaccgcca ccgtgcatga
    20521 agtggcgcgc aatgccgagg ccgcagcatc ttccaccgaa caggcggatc gccgggtcaa
    20581 cagtggcgtg actgtagtgc agcagaccct ggcgcggatc aaccaactgg ccagtgccat
    20641 gcagaccacc acccagagca ttgagcggct cagccaggac acccagcgca tcgattcggt
    20701 gctggatgtg atcaagagtg tggccgaaca gaccaacctg ctggcgctca atgcggccat
    20761 cgaggcggcg cgggcgggcg agcagggccg cggtttcgcc gtagtggccg atgaggtgcg
    20821 tgcgctggcc cggcgcaccc agcagtccac cacggaaatc gaagggctga tcgccaccct
    20881 gcgtgagggg gcgcggcgct ccgtggcgga catgcatcag agtggtgtgc tagtgacgca
    20941 gaccgtagag gatgccaacc agaccgaggg cgcgttggcg gaaatcgccg cagcagtgag
    21001 ccagatcttc gagatgaacc agcagattgc tgccgccgcc gaacagcaga cggcggtggc
    21061 ggaggaaatc agccgcagcg ttacctcgat tcgcgatatt gccgatcagt ccgcgctggc
    21121 catggacgag tccgccagct cgagcattca actggcagaa ctgggccgcg agctgcaggg
    21181 catggcgggg cgctttcgct tgtaaaagcc cccgcgagcg gctgcctagg atatttcggc
    21241 aagggctcca gggcgtttgt catggcccct agctgcgttt tagccgccgc aacagccact
    21301 tctccgcttc aacacaggcg cataccccca gcgctaccag caagcaccaa ccccaggcga
    21361 atgccggcag ggcggcggtg gcgaagacct gctgcatggc cggccagtag gtgagcagca
    21421 gttgcaggct gagtaacagg ccgaccatgc tccagatcat cgggttgtcg cgtaggccga
    21481 agttcgccgg tccttcgagg cgacggctgt tgaacaggta gccgatttcg caggcaacca
    21541 gcgcgttgac cgccagggtg cggctgcttt caaggctcca gccgagcatc tgcgtgtaga
    21601 ggaacaggcc caggctggcc aacgtcatca acaccgcaat aaaacccacg cgccagagca
    21661 ggtgctggct gagcagattg accttgggat tccttggtgg gcggcgcatc aggtcggctt
    21721 ctgccggctc aaacgccagg gccagggcca gggtcacggc ggtgatcata ttgatccaga
    21781 gaatctgcag cggggtgatc ggcaaggtca gccccagggc gatagccgcc agcaggatca
    21841 tcgcttcgcc gccattggtg ggcaggataa acaggatggt cttcttcagg ttgtcataga
    21901 ccgtgcggcc ttcctcgacc gcatgggcga tggtggcgaa gttgtcgtcg gccagcacca
    21961 tctgcgcggc ttccttggcc gcctcagtgc ccttgatgcc catggcgatg ccgatgtccg
    22021 cacgtttcag ggccggcgcg tcgttgacgc cgtcaccggt catcgccacc cgcgcgccgc
    22081 aggcttgcag gcgttcaacc aggcgcagtt tgtgcgccgg gctggtgcgg gcgaatacgt
    22141 tggtggcagg tagcagctga tccagctggc tgtcgctgag gctgtcgaga tcggccccgg
    22201 tcagcggttt gccatctccc aggccaaggc gctgggcaat ggcgctggcg gtgccggcat
    22261 ggtcgccggt gatcatcttc accgcgatgc cggcgctgtg gcagtcggcg atggcggcaa
    22321 tggcttcctc gcggggtgga tcgagcatgc cgaccagacc gagcagcacg taatcgccgt
    22381 ccaggtcctg atagtcgagg ctctgctgcg gttcgccgag cggccgaatg gccaggccga
    22441 gcatgcgcag cccctggcta gcgccgctgt ccaacgcctg atgccagtgg cgtgggtcga
    22501 gcggttcagg atggccgtcg cgccattggc ggttgcaggc ttcgagcacg cgttccggtg
    22561 cgccgatcat atagatcagg ccatggcggg catggtcatg gtgcaggctg gcggtgcaac
    22621 gttgctcgga actgaagggg atggcgtcga cttgcggcag gttgctcgct tcgtgctgca
    22681 gctccaggcc gaccttgccg gccagggtca gcagggcggc ttcggtgggg tcgccggtga
    22741 tgcaccagcc gttctcgccg ctgcgcaggc tggcgttatt ggccagcagg ccggcacggg
    22801 ccagctcgcg caggtcgtca gcggcgtcca gttggcagag caggccgtca tcgcagtgaa
    22861 tgttgccctg cggtgcatag ccgacgccat cgacgctgta atggtgtgcg gcggtaaaca
    22921 cctgctgcac ggtcatctcg ttgcgcgtca gggtgccggt cttgtccgag cagatcaccg
    22981 tgaccgcgcc gaggctttcc accgctggca ggtgacggat gatcgcccgg cgtttggcca
    23041 tgcgctgcac gccgagggcg aggatgatcg tcagcaccgc cggcaggcct tcgggaatgg
    23101 ccgccaccgc caggctgact gcggccagca gcatgtcctc tgcgctgtaa ccacgcagca
    23161 ggacgccaaa ggcaaaggtc agcagcgcca gaatgagaat gcccagggtc aggtggcggg
    23221 caaatttggc catgtcgacc agcagcgggg tttgtaggct gaccacgttt tccagcatat
    23281 ggctgatctt gccgagctcg gtctgcttgg ccgtggccac caccaagcct gcagcgctac
    23341 cagcatttac caaggtgccg gaataggcca tgcagcggcg gtcggccagg ctggtcgcgg
    23401 cgtcaacggc atcctggtgt ttatccacgg gcagagattc accggtcagt gcggcctcat
    23461 caatgcgcaa gccgcgggtc tccagcaggc gcaggtcagc gggcacgcgg tcaccggcct
    23521 ccagcagcac ccagtcgccg ggcaccagtt gctcggcggc aatgctgtgt acctggccgt
    23581 tacggcgcac tcggctttcc aagctgagca tctgctgaat cgccagcatg gcctgctggg
    23641 ctttgccttc ctggacaaaa ccgaccaggg cattgatcag caccacggca aagatcaccg
    23701 cactgtcgag ccattcgccc agcagcaggg taagcaggca ggcgccgagt agaacgtaga
    23761 tcagcaggtt gtgaaattgc agagcaaagc gctttagcgg cccgctggcc tgttgttgcg
    23821 gtagcaggtt ggggccgtgc tcgctgaggc gttgggcggc gtcggcgctg ctcaggccct
    23881 gagcgctgct gttgagatgc tgcaggcttt gttgccctgg cagggcgtgc cagtcatgac
    23941 cagatggcgc ttgcattcga tgtgctccag cgctgattga tcctttagcc taaacaccac
    24001 agacgaggag ggtgttggct tgggtcaatt gaatgggtca caagttcgtc agtaaagatt
    24061 tccagccgct atgccgtggc aacgcggcgc agtagcatcg ccagtctgtc ttaaaggagt
    24121 gatacatgcg cgccaaactg gaaactgtgc tgcagacggt caatcaagtg cttctaggca
    24181 aggagccgca ggtgcggttg gcgttgacgt gtttgttggc ccagggccat ctgttgatcg
    24241 aggacttgcc tggcatgggc aaaaccaccc tcagtcacgc tctggccagg gtgctggggt
    24301 taagttttca gcgcatccag ttcacctccg atctgctgcc tggtgacatc ctcggcacct
    24361 cggtgttcga caaggacacc gggcagttta tcttccaccc agggccgatc ttcgccgagc
    24421 tggtgctggc tgatgagatc aaccgagcca cgccgaaaag ccagagtgcg ctgctcgaag
    24481 ccatggaaga aggccaggtg accatcgaag gcgcgacccg gcctttgccc gagccgttct
    24541 ttgtaatcgc cacgcagaac ccggtcagcc agggcggcac cttcgccttg cctgagtcgc
    24601 agctggatcg ttttttgatg cgtctgtcgc tcggttatcc gggtaaggcc gcggaaaagg
    24661 ccctgctgct gggtgactcg cggcgctcgc tattgccaac gttgccggca ctgctcgatc
    24721 acgctgaact ggccctgctg caggccgaag taccgaaggt gcgtgccagc gatgcgttga
    24781 tcgactatgt gttgcgcctg gtcgatgcca cccgcaccca accgcagttc gcctggggcc
    24841 tgtcgccacg ggccagcctg gcgctgctgg cagcggcgcg ggcctgggcc ttcctcgctg
    24901 ggcgcgatta tgtgattcca gaagatgtgc aggctgtgct gccttcggta gtcggccatc
    24961 gcctgcgcga gcgcgccgat cccgccgggc atggtgccgg cagcctggtg cagtggttgc
    25021 tgcgtgaggt gccggcgctt tgatcgccca gctcaaaccg cgctggaacc gttggctggc
    25081 tcggcgcatt cctgcggctg ccagcgtacg gctcaatcag cggcggatct ttatcatccc
    25141 cagtcgggtt ggcgcggctt ttatgctggc gctgctgttg atgctgctgg cagcgatcaa
    25201 ttaccagaac agcctggcct atggcctgac ctttctgctg gcctcggtgt tcgtcgtggc
    25261 cattctgcac acttaccgca acctggccgg gatgatcctc aaggctggca gtgcgccggc
    25321 agtattcgtc ggagaacccg ccggttttcg cgtgcgcctg gaaagcagtc agcggcccca
    25381 tcaggccgtc agcctgggct ggccaccggc cgaattgcag acgctggatg tgccagctga
    25441 aggcgttagc gagtgccagt taaacctacc ggcgttgcag cgcggctggt tgcggcccgg
    25501 ccggctgcgg gtcgagagtc gtttcccctt ggggatactg gtggcctgga gctgggtcga
    25561 tctggatcag cgtgtgctgg tttatccacg gcccttgccg ggtgatctgc cgctgtccgc
    25621 cggcgccagt gaggatcagg acgaggaggg cagccgaccc catggccagg gtgtggacga
    25681 ttatcaggga ctgaaaacct accagccggg tgattccaaa cgccgtctgc actggaaagc
    25741 ctattcccgt ggccagggtt tgctggtcaa ggatttcgcc tcgatcactg ggcgtgatct
    25801 gtacttggat ttcgaggcag tggcgggcga tgtggagact cgcctgtcgt tgctctgtca
    25861 ttgggtattg cagttgtccg agcggcagca gccgtttgcc ctgagcctgc cgggcctggt
    25921 gctgggagcc gacagcggcg agctgcaccg cgacgcctgc ctgcgcgccc tggcgctgtt
    25981 cgggcgtgcg ccatgagccg tattgccgtg atcccgcgga tcagcctgac ctggttgctg
    26041 gtggcgcagg tgctggtgat cattcctcac ctgacgcatt tgccgctatg gatcatcggc
    26101 ctgtggctgg gcagcgcggc ctggcgcatt cagattttcc gcatgcgcgc acgctacccg
    26161 aatgcctggg ccaagggtgg attgatgctg gctgcggcgc ttggggtgtt tctttcccgt
    26221 ggcagcctga tcggtctgga tgccggggtg gtgctgctga tcgccgcctt tgtgctcaaa
    26281 cttctggaga tgcgcacgcg gcgtgatgcg ctggtgctga tctttctcgg cttctttgtg
    26341 gtggtcactg cttacctgtt cgatgacagc atgttgatgg ctttctacag cctgctgccg
    26401 gtcaccgcct tgctcgcagc cttgattggc ctgcagcaga gcagcctggc cattcagccc
    26461 tggccgaccg cgcgcctggc cggcgggctg ctgctgcagg cgctgccggt gatgctgctg
    26521 ctgtttgtat tcttcccgcg catggggccg ctgtggtcct tgccggtgcc gaatgacaaa
    26581 gccactaccg gcctgtcgga cagtatggct ccggcggata ttgccgatct tagtcgctcc
    26641 gcagccctgg cgtttcgcgc cagtttcaat ggtgaaaccc cgccgcgcag tgagctgtac
    26701 tggcgcgcgc tgaccctgga tcgcttcgat ggacggcgct ggtcgcaatc cgaatacgcc
    26761 aagcttggcc ccgctgcgca gtggcagaaa cgtggtgagg cgctgagtta cagcgtcgtg
    26821 atgcagccca gtgcacagcg ctggctgttt gccctggatg tggcggaaac cacgttggaa
    26881 caggcccggc agatgagcga tttccgtcta cagcgtcggc aaccggtgga tcgctcgctg
    26941 atctatcagg ctacctcttg gccacagggg ctgcgcgaac cgcaactgcc ccaggcagcc
    27001 cagcgccgtg ccttacagtt gccggatcaa ggcgagccgc gcagccgcgc ctgggctgag
    27061 cagctcaagc gcgagcatcc gcagcccgag gagctggtgc aggcgctgct tagccacttc
    27121 aaccgcgagc cctttgctta caccctgacc ccgccaacat tgggggtaaa caatattgat
    27181 gattttctgt tcgaaacccg cagcggcttc tgcgcccact atgccggggc catgacctat
    27241 gtgctgcgtg ctgcgggcat cccggcgcgg gtggtggccg gttatcaggg cggcgagttc
    27301 aacccggcgg gcaactacgt gcaggtgcgt cagtttgatg cccatgcctg ggtcgagtac
    27361 tggcaagagg ggcagggctg ggtcagcgtc gacccgacgt ttcaggtggc gcccgaacgg
    27421 atcgacatgg gcttggaaga ggccctggcg ggtgagcaga gctttcttga ggattcgcct
    27481 ttctcgccgc tgcgctatcg caacctcagc tggctgaatg agttgcgctt ggcctgggac
    27541 aatatcaatt acggctggca acgctgggtg ttgggctacc agagtcagca gcagctggag
    27601 tttctccagc gctggttggg gcgggtggat gcgcagtcgc tggtgatcgg tctggtcggt
    27661 ggcggggcgc tgctgctcgg tctgctttcg ctctggctgt tcaagccttg gcagcgtgag
    27721 cgcgatgtgc agcagcgctt gttccgccgt ttcgaggggt tgctggcgcg tcatggcgtg
    27781 ccacgtaaac ccggcgaagg gccgcgcgac tatgccacgc gggcagctct gttgatgccg
    27841 acccaagcgc agtcgattgg cgactttgcg gcggccttcg aggcgcaacg ttatgcaggg
    27901 caggagcctt ctgccctgca attacgtcag catttgaagg cgctgcgccg ggcgttgcct
    27961 tggcgtttta cccgtttgga ctcatagcga ccattggcca cttgtggccg ctcaataagc
    28021 gcataagctg agcagcgatt tcacaggagt gaggcgatgc aagacttgat tgatgaggtg
    28081 ccgcgacacg tactgcgtat tcagatgcgc ttgttcgcct gtcgtgaacg gctgcgagcg
    28141 ggcaccgacg atgaggcctt gcacgatctg cgtatcgcct tgcgtcaatt gcgcagcctg
    28201 ctgcggccgc tgtcctcggt cgcagtctgt gaggacttgc aacagcgtgc ggccgagctg
    28261 ggccgcttga ccggtccgct acgcgacctt gaggtgctgc aaggctatct gcaacagcaa
    28321 gggctcgata gcgccgcgca atcgcgcctg cctcttttgc agcagggcta cctgcagctg
    28381 ttggacagcg cgccgttggc gcatctgctc aatgcattgg atgactggcc gcagcagtgg
    28441 cgtcaggcgg ccaacaacgg gcaactgcgt ggcctgcaca agctgatcaa gcaacgcttg
    28501 cgcaaggatc acaagcatct ggcgcaggcg ctgcttgatc ctgcccatga ccgtcaccgc
    28561 ctgcgcttgc tggtcaagcg cctgcgttat gccgccgagg cctatccgca gttggccgat
    28621 ttgtccgggc gtatgcgcat gcagctgaaa caggcgcagt cagcgctggg cgattggcac
    28681 gaccactggc agtggctgct gcgcgcggaa gccgaagcgg atctgcagcc ctgtatgccg
    28741 gcttggcgtg aagcgatgca gagtgccgag cagcaggccg atctggcctt gcaggcattg
    28801 atcgcgggtt ttgctcgcag cggctgagtt ggcggaaaag tcgttatacc ttcggcttgt
    28861 ttggccgaaa tgtcgtgtca cccatagccg cctttgccct aagatcaccc gcatcttcag
    28921 tgttcgaggt ggttatgggt ttttccgaat tactccaggc ggtgcgcgac aaccctcagg
    28981 cggtgatgat tcccgcgcag tgggggcagg gccgcgccag ttttggcggc ttggtgacgg
    29041 cgctggtgta cgaagccatg cgcgctcagg tgccgagcgg gcggccgccg cgctccctgg
    29101 caattacctt tgttggccca gccgcgccgg atgtgccggt gcgctttgaa gtggaagtgc
    29161 tgcgcgaagg caaggccgtc agccaggtgc tgggccgggc gatacaggat ggtcaggtgg
    29221 taaccctggt gcagggcagt tttggtgccg ggcgcgagtc gcgcatcgcc gtgcaggccg
    29281 atcccgcgcc gcaggccaag gcggtggagg attgcccaga gctgccgtat atccccaacg
    29341 tcaccccgga gttcacccgt tttttggcca tgcgctgggc gtttggcggc atgcctttta
    29401 ccaacacgcc atcacgggag atgggcggct gggtacgctt gcgtgatcaa ggcgttgctg
    29461 aggcggccaa tgaggcccat ctactggcct tggtggatgc ttggccgcct gcggtgttgc
    29521 cgcatttgag cgcaccggcg ccgggcagct cactgacctg gaccatcgag ttcgtccagc
    29581 cgatgagcga cctgagcacg ctggattggt gcctgtaccg cgcgcagatc gagcatgccc
    29641 gtgatggcta cggccatgtc gcggcggcgc tgtggtcgcc ggccggtgaa ttactcgcga
    29701 tcagccgaca gaccgtggcg gtatttggct agagcttgag ctggcgaatc gccttgttta
    29761 gctcgccagc caggttggcc aggtcggcac tggtaatcgc cgactgaccg gtctggccga
    29821 cggtctgttc ggtcacgtcg cggatgcgga tcaccgcgcg gttcatctct tcggccacct
    29881 ggctctgctg ttcggccgcc acagcgatct gcgtattact ttcccgcata agcgccacgg
    29941 attcggtgat ggccaccagc gcctgacctg cttcaccggc ctgtttgacg cattcatcgg
    30001 ccttgatcga gctgtcctgc ataaagtcca cggcatcacg ggtgccgctt tgcagggtgt
    30061 tgatcatctg ggtgatcacg tcggtggagt cctgcacgcg cttggccagg ttgcgcactt
    30121 catcggccac cacggcaaaa ccgcgaccca tttcgccggc tcgggcggcc tcaatcgctg
    30181 cgttcaacgc gagtagattg gtctgttcgg caatgccgtg aatctcactg accacgccgc
    30241 cgatcttttg gctgtcgacc gccagctgtt cgatcatctg cgcggtctgc tgcacgccgc
    30301 tggacagccc ggaaatcgac aggctcacgc ggttcaccgc ctgctggccg ctgcgcgcca
    30361 gctgttcggc gttttttgac tgatcgcggg tatcagccgt gtgctcggca atatggtgca
    30421 cggtggcaga catttcattg atcgccgtgg ccgcctgttc ggtttcgctc tgctgttcga
    30481 gcatgccctg gcgcacatcg cgcatattgc cggccaggcg ttcggcaccc tcatccagct
    30541 gtgctgccga ctgggcgaca atgccgacta cgcgctggta accggcctgc atggcattga
    30601 aggcgctggc catctggccc acttcatcct tgcagtccag tggcacgcgg gcactgaggt
    30661 caccgctttt ttccacctgc agcatcacgt ccttgagggt gttgaggtgg ctgagcagaa
    30721 agcgaatcag caactgcgag gccgccagca acagcagcat caacagcgcg acaaccagtg
    30781 catagcttgg cgcacggtcg aaaaacactt cggaaaggct tggtgcggag gctgtaattg
    30841 ccaggtgctg accgtcttca cgaacgatga cttcggcgcc gatcagccca tcgctaccgt
    30901 ttaaatcgac ccaaccacgg gcgttagcca gagcgctgcc ctgattatcc ggcaagcgtg
    30961 gagtctggcc ggctgcaacg ctcagcaggt tgctcgcgct tggcaggggt tgctcctgcg
    31021 gccattggct taataagcgt gcctgctgct gcgcagcctg cttggcggcg gtgtgatgca
    31081 cctgctgctc cagctgcagg gcatagagca ccagcagcag cgtagtaacg aaggccacgg
    31141 catttacggc ccagaatttg tacttcaggg aaaggtcgct gatccaatgg cccatgcagg
    31201 tgtctctgtg tgaggtgaag gcttggcaaa gtgccagtag tgtcggtcaa cgccactgtg
    31261 gcgctattga tccttatcaa caagctagca ttcactgtgc tactcggcga tcattcggcc
    31321 gtgcccttga atgcgggtag cttgaaaaag gcctgtgccg cagcgctggt atgtgcagcc
    31381 agtacttgct cggtttcacc acggtgcagc gccacttcac gcaacacctc aacgagaaac
    31441 gccggttcat tacggccgct cttcggtttc ggcctaaggc tacgcggcaa caggaagggc
    31501 gcatcacttt ccagcatcag ccggccacgg gggatttcct tgaccagtgg atgcaggtga
    31561 ctgccacggc gctcgtcgca gatccagccg gtgatgccga tatgcaggtc catatccagg
    31621 taattgaaca gtgcgcgctt ttccccggta aagcagtgca ccacggccgc cggcagctgg
    31681 tcgcggaaat gccgcaggat cgccagcagg cgctcgtcgg catcgcgctc atgcagaaac
    31741 accggtaatt gcagctccac cgccaaagcc agttgctgct ccagcaccag ttcctgttgc
    31801 gagcgaggtg agaagtcgcg attgaaatcc agtccacatt cgcccaccgc gcgcactctg
    31861 ggattggcca gcagggcctt gagctgcgca tggctgtcgc tgttccagct gctggcatcg
    31921 tgggggtgaa tgccggcagt gctgaacaga tgctcggcgc ggtcatccag ctgccggcac
    31981 agctccagag cgtgctgact ttccgtcagg ctggtgccgg tcagcagcat ttgctggacg
    32041 cccgccgcat aagcgcgagc gaggagtgct tcgcgttgtt cggcaaagct tgggtgagtc
    32101 agattgacgc cgatgtcgat gagttgcatg gtgctacctg ctgaattgat tgggcagcat
    32161 agcagagctt ttttaataga ttaaaagcta accaatacag ctacttaaag cttgaatgtg
    32221 atgttgtgtg tgattgctgg ctagcatcca tgagcgggct gtgcgaccct ccgcgccccg
    32281 cgcgaggggt cagttgcctt atacgcagcc tgttcagacg agccgctgcg ctgatctgtc
    32341 ggtcgattct gccctggagc cttgatgttg cgagtgctgc tcacctgctg cctattgctg
    32401 ctgtcttgca cagcggtggc gcgcgtggct ggcccgctgg aagtcgagca gcccaaggcc
    32461 gcccgtgacc tggcgagcat ccgcaacagt ggtgaattgc gcgtgctggt caatcagagc
    32521 cgcaatagct caggcgaggt caaaggccag agcatcggcg tcgagtacca gcgcatgcgc
    32581 gcattcgagc agcacctcaa tcgcaactcg cgtgatggcc gcacactcaa actgaagatc
    32641 attcctcgcg ccaaggatca gctgctggct gccttgcagc gtggtgaagg cgatctggtc
    32701 gctcccggtg agctgctgaa tatgccgcgc ggcagcaagc tcagcgccag cgcggccatc
    32761 cgcagcaatg tgccgatgat catcgtcgcc aaacagggta accgtcgtta ccagaacctc
    32821 gaacagctct ccgggcggag cctggctttg cccgcgggca gcgtggcagg ggaagcggtg
    32881 cgggcggtca atcagcggct gatcgacagc aagcgttcgc cgattattgt cgagtgggtc
    32941 gaccccagcc tggcagtcga ggacgtgctg gaaatggtcc aggccggcat cttcagcgtc
    33001 acggcggtcg agcagccgat tgccgagcgc tgggccaagg tgatgcccaa gctgcgtctg
    33061 gatcgccacc tgcaattgac caacgacggt gatatgcgtt ggtacgtgcg ccgcgacacg
    33121 ccaatgctcg gcgcaagcat cgaccgcttt ctcaagggct atcgtgatcc agcggatcag
    33181 gatgcagcgt tccagcgtgt ctaccggcgg ttgtacaagg tgcattaccc gctgggccgc
    33241 actgaacgcc agcgcctgga aaaagtccgc ccggtgctgc agcgctacgc caatcaacat
    33301 gatttcgact ggctggcctt ggctgccgtg gccttcaagg agtccaccct taaccccgcc
    33361 gcgcgcgggg ccggtggcgc gaccgggctg atgcaaatca ccccggcggc cgcacgcagc
    33421 gttggcgtga gcaatatcag cgtgctggat aacaatgtgc aggccagcgc caagtacctg
    33481 gcaatgattc gccgcaacta cttcaatagc ccgcagctca atgagcgtga gcgcatggcc
    33541 tttatcctgg ccggctacaa catgggcccg caacgggtgc agagcctacg ggcggaagcg
    33601 cgcaggcgcg gcctgaaccc caatcaatgg ttctttcagg tggagcgggt ggcgatggag
    33661 cagctgggca tgggcgtggt cagctatgtg aatagcgtga ataagtacta ccttgcttat
    33721 gaccgcgagc gctatctatt ggagccctag cgcgcaggtt gagaatgtcg atttattaat
    33781 ttattcgatg tgttatagcg attttattcg ctttaattat tgattaattg gtttaatctt
    33841 cgcccatcaa ctccccacta caaggactgc tgcacatgaa cgctctgatc aaatctgttg
    33901 ttgcaactca agctggctat ggcctgaccg tgctgcgcat catcgccggc atcacctttg
    33961 ccgcccacgg cgcgcagaag ctgtttggct ggtttggcgg ttacggcctg gaaggcgtag
    34021 gtcagtggat ggaaagcatc ggtctggcgc cgggctatct gttggcactg atggccggca
    34081 gcgctgagtt ctttggtggt attgccttga tcatcggtct gctggcgcgc ccagctgcag
    34141 cggtgctggc gctgaccatg gtggtggcga ttgccactgt gcacctggcc aacggcttct
    34201 ttatgagcaa caacggttat gagttcgccc tggcactctt ggcgatcagc gtagcgatcc
    34261 tgattgaagg cgctggcaag ctgtcgctgg acaagcgcat cgctggttaa gcgactcggc
    34321 agcagccagt aaaaagccca cttcggtggg ctttttgcat ttcagggctg gggcttgttc
    34381 tgcgtcgcca actgttcggc ggcctccagg cgcagttgtt cacgctcatc gaagttctcc
    34441 gccgccagtt gggcaatggc tgcacgtttg tcgctggcgc tcatgccggt gttgttttcg
    34501 atggcggctt tttcagtgct gtactgggcg atgcgctttt tccaggtttg tcgctgctgg
    34561 tcgagcactt cgaggcgctg ggtggcttcg gcgccgacca gttgctgacg cagctggcgg
    34621 atctgctccg gtttggcacc ggctgcttgc aattgcgcgg tttgctggcg cagctcgttc
    34681 tgcagttgcg gcagcacggt gtcctgcatc tcggcaggca ggctgttgcg cagttggtcg
    34741 acggcggcgg ctttgccggc atcgtccagc tggctgttat gcagaatcgc caggcgctgc
    34801 agggtgaact ggttgtaggc ctcttcgcgg gcaaagaacg cctgttgggt ctgggcatcg
    34861 aagatgcgcg cgcgcaaagc ctgtacggcg gcctcacgct gacgcagggc atccaggttg
    34921 gccatctgcg gcagatcacg ttcgagcagc accagctcgc gtttgtagtc gaggtactgg
    34981 ttaagcagcg ccagggcttg ctcccgggca ttggccggta gttgtgcggc gatgtaagcg
    35041 cgcagccttt cgacactggt acgcagtggc tcttcaccca ctgcggcgag gaaataatcg
    35101 aagatccgcc ggatgtcttc gctgatcagc aggttgccat cggcatccag gcgaaagacg
    35161 ccatccaccg tggtgccgcc gaatgaggcg ggcagggttt tcaagggatc agccgcgttc
    35221 ttcggcttgt gctgggtttg attggcgggc gctgagtccg cgctttcgac ggcaggcgcc
    35281 ggcgtatcag cgccgagctg cgcgggaata tccacgggtt gcaggtacag cgcgagggcc
    35341 aggcaggcgg cgaatactag tggggacaac aacagaagtt ttttcacagg cactcttcaa
    35401 gcgggtttgg cgaggcgagg cagaagaaac gccgggggct gtaacagccc ccgacgaagc
    35461 tgttacagcc cgtcgttttt caggcgatta gcgtgctggc gatagaccgt gaccgggtcg
    35521 gtctcgaaca ggctggtcag accgagggtc tggttgacct cgtcgaggtg gttcatccgg
    35581 tagttatcgc ggatcacctt gcccatgcgc gagctgcagc gaccgaccag accatcgttg
    35641 gcttcaccgt tgaaggtcag cgcggtggcg cccagcagca ggtcgctcgg gtcaatgata
    35701 ttggtcaccg ggctggtgcc gctccaggag tagtagcgca cgccgccgac gctgtaggca
    35761 ccttcaccgc aggcgctggt aggaatgccc tgcgggaact tggcgttaaa ccgtgctgca
    35821 ccttggctgt taagtgattc cagggaaccc agggcgttct gcgggctggt gctggagctg
    35881 ccagagagga agttgatcaa cgtgccgagg ccgttgacga tgccggccag caaggtttcc
    35941 ccggccgagc ctggcggcac ctgacggata aaatcggcag ttgccgagcc tttatgcggg
    36001 gccccgacgc tggtggccga agcaatcagg ttggggctga cagcggcgac atagcgaatg
    36061 gtcggcccgc catggctatg gccgatcagg ttgaccttgg gcttgccgct gatggcgacg
    36121 atgtcttcca cctgggccag cagttcttcg ccgcgggctt cagaggtgtt cagctggctg
    36181 acttcagtga tgtacacact ggcgccgtcg cggcgtagcg ctgcaggaat gccataccag
    36241 taatcgacac caaggaggct gtcgaagccg agcataccgt gggccagtac gactgggtat
    36301 ttggtctggg tgtagccgga ggagccgaac cagaaagcat gggtgttgcc agaggccagc
    36361 aagccggcgc ccaggcagag ggcgagcaga gttttattgt tgttcattgg tgctctcgct
    36421 agggttgatc gggcagttca aggcgctccc tgcagccaaa acgcccatcc cgtggcgcgt
    36481 acatcagctc aagggtagtg gcagaaagca tgccagccag tcgcaccggg cttgaaaggc
    36541 tctgccacgg gcgctgcggg gctgtcgtgc ttggttcggc aatcgcgcac tggcatgcgg
    36601 ttgttacagg gtgaaacgac gcaggcctcg atacagaccc catatgaatg gccagcagct
    36661 cgtatgccag cggcaaaaag ccagtttggc cggccgaata gtggtgccac cagtcaaagg
    36721 ttggggtggt tggatagggc cgcgggatag agccttgaca gctttgcgcg ggcttctcta
    36781 ggatgctgac ccatgcgccg atttaaacag ctacttgcgg ggcgccgggt tacctgctga
    36841 ttgcagcgcc ctaagaagca ctcttcggag gcttcgaaat tccgctaaag cgctggttcg
    36901 gtgtcgcctc tcaccgctgc ccagcggact caatgaggcg gagacatatc gatgcactgt
    36961 ccccaaccgc ttattcgtct ggccccgatc acctctgggc tgaccctgcg caatccgaaa
    37021 attctgcttg gcggcagcca ccagccaacc ttgctgcgtt acctcgatgg ctggcccaaa
    37081 cgttggccgg ggccgcggac ctttctgatc aactttgttc atgacggcga gtctctcgcg
    37141 cgatttgcca gcaatcactt cgatatggcg gtgattgaag cgccggccgc cgcgcagctg
    37201 gaagaaaccg ttcgccagct gacgcgcgtg gccaagcagg ggttgatcac gcggcgctga
    37261 gtgaatcgca caacggcttg gccacagcca ctgccgatcc gctaggctca aacgatattg
    37321 tcagggagtt ggctatgttc gaatccgccg agatcggccg cagcatcgat aaagaaacct
    37381 atgaggccga ggccccagca ctgcgcgaag cgctgctgga ggcgcaatac gagttgcagc
    37441 agcaaggacg ctttgccgtg cttatcctga tagatggcat cgagggcgcg ggtaagggcg
    37501 agacggtcaa actgctcaac gagtggatgg atgcccggct gattcaggtc aataccttcg
    37561 accagcagac tgatgaagag ctggcgcggc cgccggcctg gcgctactgg cggcaattgc
    37621 cgcccaaggg gcgcatcggc atcttcttcg gcaactggta cagccaaatg ctgcaggggc
    37681 gggtgcacgg gcggatcaag aatgccgtac tcgatcaagc catcgacagc accttggggc
    37741 tggagcgcat gctctgcgat gaaggcacgc tgatcttcaa gttctggttt catctgtcca
    37801 agcagcagat gaaggcgcgc ctcaaggcgc tgcaggacga cccgctgcac agttggcgga
    37861 tcagcccttt ggattggcag cagtcgaaaa cctacgacaa gttcgtgcgg gttggtgagc
    37921 gcgtattgcg ccgcaccagt cgtgattacg cgccctggta tgtggtggag ggcgtggatg
    37981 agcattaccg cagcctgact gtcgggcgca tcctgctcga tggcctgcaa gcggcgctga
    38041 aggcgcaaag ccagccgttg ccacatccgc atgccgcgcc gctgacctcc agcctggatg
    38101 agcgtgggtt gatcgacagc ctggacatga cccaggcatt ggacaaggac agctacaacg
    38161 agcaactggt gatcgagcag gcgcggctgt ccagcctgat gcgtgacaag cgcatgcgcc
    38221 gccattcgct gatcgcggtg ttcgaaggca atgatgcggc gggtaagggc ggggcgattc
    38281 gccgggtgat cggcgcgctc gatccgcgtc agtaccgcat cgtcccggtt gccgcgccaa
    38341 ccgaagaaga gcgcgcgcag ccctatctgt ggcgtttctg gcggcatatc ccgccgcgcg
    38401 gcaagttcac cgtgtttgat cgctcctggt atggccgggt gctggtggag cgggtcgagg
    38461 gcttttgcag tacaggcgac tggctgcgcg cctatggcga aatcaatgac ttcgaagagc
    38521 agctcagtga agccggcgtg gtgctggtga agttctggct gtcgatcgat cagcaaaccc
    38581 agctggagcg cttcaaggcg cgcgagcaga ttcccttcaa gcgtttcaag atcaccgaag
    38641 aagactggcg caaccgcgat aagtggccgc tataccgcga tgcggtgggc gatatggtcg
    38701 accgcaccag caccgaaatt gccccgtgga cgttggtgga agccaatgac aaacgctttg
    38761 cccggatcaa ggtattgcgc accatcaatc aggcgcttga ggcggccttc gccaagggct
    38821 gagcggcggc tgcgcatggg cacgggatgg tcgcgcatac ggctgatgaa tgatcgtcgc
    38881 cgagcgagat tgtccggctt tattcttgtt ggcaactgaa cccaataaca atgaggtgct
    38941 acatgcgtga agtggtgatc gttgacagcg tacggactgg tttggccaag tccttccgtg
    39001 gcaagttcaa tctgacccgt cccgacgaca tggcggctca ttgcgtcaat gcactgttgg
    39061 cgcgcaccgg tatcgatcca gcgctggtgg atgattgcat tgtcggcgct ggttccaatg
    39121 aaggcgccca gggcatgaac atcggccgca acgttgcggt actgtccggc ctgggcacca
    39181 atgtggctgg tatgaccctc aaccgtttct gctcctcggg tctgcaggcc attgcgattg
    39241 ccgccaacca ggtggcgtcc ggctgcagtg atgtgatcgt ggcgggcggc gtcgagtcga
    39301 tcaccatgac catgaaaagc atgaacacgc agaacctgtt caacccgcta ctacaaaaag
    39361 atgtgccggg tatctattac cccatggggc agaccgccga gatcgtcgcc cgccgttaca
    39421 acgtcagccg cgaagcccag gacgcctacg ccctgcagag ccagcagcgc actgcacggg
    39481 ctcaggccga aggcctgttc gacgatgaaa tcgtgccgat gcacgtcaag taccaggtcg
    39541 aagacaaagc cacgggcgag aagagcatcg tcgacggcat cgtcgcccac gacgactgca
    39601 accgcgccga caccactctg gaaagcctgg ccgcgcttaa gccggtgttt gccgacgacg
    39661 gttcggtcac cgctggcaat gcctcgcagc tgtccgatgg cgcgtcgatg accttggtga
    39721 tgagcctgga aaaagccatc gaactaggcc tcaagccgct ggcgttcttc cgcggcttcg
    39781 ttgccgctgg ctgcgaacca gatgaaatgg gcatcggccc ggtattctcg gtgccaaaac
    39841 tgctcaaggc caagggctta agcgtggccg atatcgattt gtgggaactc aacgaggcgt
    39901 ttgcttcgca gtgtctgtat gcccgcgatc gtctggaaat cgacaacgcc aagtacaacg
    39961 tcaatggcgg ctcaatctcc atcggccatc cgttcggcat gactggttcg cgccaggtcg
    40021 gccacctggt gcgtgagctg cagcgacgca acctgcgcta cggcatcgtc accatgtgtg
    40081 tgggcggcgg catgggcgcg accgggctat ttgaagcggt gcgctgatca gcggcgccat
    40141 aagtggcagc caataaaaaa ccgcgagtac tcgcggtttt ttattggctg gagttttgcg
    40201 ttttagaacg cgcggctctc gatgatctcg ttagcctgaa tcatccggcg gatataaaag
    40261 gcaatctcgc gctcggcatc cgttcggttg aagtagggcc cttccaacgt gccctcgcgg
    40321 gtggagaaga agtattggcc gttgaccgcg ctaacgcgtt cgctgcggta gtgggtgccg
    40381 gggttatggt cgatatggcg ttggccgaac ataaataaac ctcccgcaaa atgagctgct
    40441 gaggtcagtc taaacggcgt ttttctggct gcatgaaata acgacaaatg gtcgtttttg
    40501 ccgaaattcg gcgtcttttt cctgaattta atattttttg acgccagaac cgtggctttg
    40561 tgtcgcgtgg ccatcagtac agtgcccggt gtcgcggcac ggagctgtgg ctgtgccggt
    40621 ttttaacgct gcagagcgtt agccggttct caggcgcgca cacccagtgc ttcggcgcgg
    40681 cgtagccagc tctggcgctg ctcttcggat gaagggcgta ccggcgcgaa ttcggccagg
    40741 cggcgggttt tgatgccgct gaagccgaga atggtgcgag tcatctgccg atgcccaggc
    40801 gcgccgtaga tccagcggaa gtaccagggc ggcgtgtcca tggtcaccag caggtcggca
    40861 gtgcgcccag tcagcagctt gtcccagagt tgcgagcggt tgcggtactt gaaggcaaag
    40921 ccgggcagaa aggtgcggtc aaaaaagcct ttgagcagcg cgggaatgcc gccccaccag
    40981 accgggtaga cgaataccag atgctcggcc cagtggatct gccgctgcgc ttccagcagg
    41041 tcgggttcca gggtttggct ctgctcgtaa ccgtcgcgta gcaccgggtc ataactcatc
    41101 tcgcccaatt tcagctggcg taccacatgc cccttgctgc gtgcgccctg ggcataggct
    41161 tcgccgaggg catgacagaa gctgctgttc ttcggcgtgc cgagaatcat caggatgcgt
    41221 ttgccgtcac cttccagtgg cgttgcgccg ctctttgccg cttcactcat gttgtcctgc
    41281 ctccagcatg ttttcaggac gcacccaggc gtcgaattgt tcattgctca ggtagcccag
    41341 ctgcagcgct gcttcacgca gggtggtgcc atcctcgtag gcctttttgg cgatctgtgc
    41401 ggctttgtca tagccgatat gcaggtttag cgcggtcacc agcatcaggc cgcgctccag
    41461 gtgctcggcc attttcgccg catcgggttg caggccagct acgcaatgct gctggaagtt
    41521 atggcagccg tctgcgagta agcgtatcga ttgcagcagg ttgtgaatga tcaccggctt
    41581 gaacacgttg agctgcagat gcccctggct cgcggcaaag gcaatcgtgg tgtcgttacc
    41641 gagaacctgg caggccagca tcgacagcgc ctcgcattga gtagggttga ccttgcccgg
    41701 catgatcgag ctgcccggct cattggctgg caagcgcacc tcggcaaagc ctgcgcgcgg
    41761 gccggagccc agtaaacgca ggtcattggc aagcttcata agggcgaccg cgagggtttt
    41821 caaggcgcct gatagcgcca ccagcggctc gtggccggac agggcggcaa atttgttcgg
    41881 cgcgctgacc agcggcaggc cacttaaagc ggccagttcg gcggccatgg cgtcggcaaa
    41941 gccctgcggc gagttcaatc cggtgcctac tgccgtgccg ccttgggcca gttcgcagac
    42001 tgccggcagg acagcctgga cggcccgctc ggcgtagccc agctgtgcga caaaggccga
    42061 tagctcctgg ccgaaggtca tcggtgtggc gtccatcatg tgtgtgcgcc cggtcttgac
    42121 cagccgcgca tggcgctcgg cctgctcctg caaaccgtcg cgcagttcac gtacggccgg
    42181 tagcagttgc tgttgcacgg cctgcacggc ggcgatatgc atggcggtgg ggaagctgtc
    42241 gttagagctt tgcgcgcgat tgacatggtc gttggggtgc accggcgtct tgccgccacg
    42301 gccgttgccg gccagttcat tggcgcgtcc agcgattact tcattgacgt tcatattgct
    42361 ttgcgtgccg ctgccggtct gccagaccac cagggggaat tgttcatcgt gctgaccggc
    42421 cagcacctca ttggcggcct gctcgatcag tcgggcgagg tcggccggca cttcgccgat
    42481 acgctgattg acccgcgcgg cggccttctt gattagcgcc agggcatgca ccaccggcaa
    42541 gggcatacgt tcctggccga tggcgaaatt gaccagtgaa cgctgggtct gcgcgcccca
    42601 gtacgcctca tcgggtactt cgatggggcc caggctgtcg gtttcagtgc ggctcatggc
    42661 ggctctcctg atggttgcgc gtgcagcaaa cggactgcca ggccggcgat tgccagcctc
    42721 ggcgtatctc agcttaggtc gctatgcatc agtagaggtt caatagccgc aggtaactac
    42781 cagcgggcgc gggtctcacc gagccagccg agggcgttgt tgactggcac gcgctcacct
    42841 tctggaccgc gcagaacacc ctcgaaatgc ccgaaccact gctgcgtatc ggcatagaac
    42901 ggcccaagcc ggcggtaggc ctggtgacgt tggcgtgggg tgaaggtcag ctgtacggca
    42961 tcatcagggc tgcgcagctg ccagggcgcc agcgggttgt gtggtgcctg ggtaatggtg
    43021 atgggcgtat ccaggtgctg cagttcgccg ccaaaccaca gggtgttctc ggataggccg
    43081 ctgtggtccg tccagccaga gccgaagttg ccggcaatac cgccgggcgc ggcgaaggcg
    43141 gcgcgggtcc agtggctgtt gagtggccag acgccacggc cgaaatccag cgaggcaaaa
    43201 ctttgcccgg tgacgcagct gtaatgctgt ttgcccagct gcacgctgcc actgcagggc
    43261 aggcccagct ggcggctggt ggcgtgaaag ctatgcgggc cgagcggggc gaccaaattg
    43321 accgagtcca ggtgggcagg gcgctgaata tccagagcta cctgcagcgg ttgcccgcca
    43381 atatccggcg cattgatggt gatgcgcgtg cgcccggtgt gttcagctac acgcagttgc
    43441 aggcgtgggt ggttgaattc gtggctggac tgcggttgat ctggtaagtc gcagccaagg
    43501 ccgaacaggc ggtactggct gtaggcgacg gcttgaccgc tttgcagatc aaggaagtag
    43561 gcggcagcgt aacccaggta gtcgaggtcg gcctgggtaa acgagagcat ccactgcggg
    43621 gtggtgatgc accaatggtt ccagcgttta cgccggccga agtgcccggg tatcgcacaa
    43681 tcgaccatgg gtctgggcga ccagccgatg gcttgcgggt taagcaggcc gtggctgtcg
    43741 catagcgcga tggcggcaag aggctctgga tgagtgaaac tgttcatcga cacgtccatg
    43801 tgtgtgctgg cgcctcgcct tgtgggcggc ggcacgctga aacagaagat gatttacctt
    43861 gtcgagcatg cacaggcgtt ggtctactcg gcaagtgctg gcattttggc cttttagcaa
    43921 gcagtgcgcc agtgcacagt tgtagtgtga gttgatcgac gcggctcact tacttgagtg
    43981 tgaccagcgc ttgggcacaa aatgccgata atcgagtcca acaggactca ttcccttccc
    44041 caggagtagt acatgtctcg tttacctgcc ttttgcgctg ccctaatact gacgtgcggt
    44101 agtgctcagg tactcgccga tgccaaaagc catgccgctg acgccgagcg tttcctgctg
    44161 ctggcccgtg ccgacaagct ggctgtaccg gtgtatgccc aggtacagca aatgtttgcc
    44221 cagcgttttg ccgagagcaa tgcgccgcag agcgagaagg ctgtgctgga aacctaccag
    44281 gcccaggcca atgcagccct tgagcaggcc gtgggttggg acaagctcaa gccggatatg
    44341 gtcaaactct acaccagcaa cttcaacgag caggaaatga aggacctgat ccgtttctac
    44401 gagtcgccgc tgggcaagaa agtcctggag aaaatgccaa cgctgaccgc acagtcggcg
    44461 cagctgaccc agggcaaact caaaggcgcc gtacccaagg tcaaccagct gttggcggaa
    44521 atgaccacca aactgactcc gaagaagccc tgagtgactg taccgatgcc gaagatcgat
    44581 ctgctgcagg ccgctctggc aagcctgcag ccccagcacc tcgatgtgtt ggatgaaagc
    44641 catatgcaca gccggggtct ggaaacccat tacaaggcgg tgatcgtcag cgagcagttc
    44701 gccggcctga atgcggtcaa acgccaccag aaggtctatg ccgccgtagg tgagctgatg
    44761 ggccagatcc atgccctggc gctgcatacc tacacgcctg atgagtggtc gcagcagggc
    44821 gtggctccgg cttcgccgac ctgcaagggc gggcactgag aacctgctta cgatctcgcg
    44881 agctagagac cagctaggca aaagcaggcg aggaagcgga gtgtacgagt tgtacatgag
    44941 catccgagtc tgcttttaac gcagcagggc cgacgcgcag cagatcgtaa gcaggttctg
    45001 aggcaattcg cctctacacg gttcccctca agggcagtaa actgcgcacc ccgccggcct
    45061 agcgccggcg gttttgtttg tgtatcccgg tccgcccttt acgtgggcga ctactgaagg
    45121 gtttgcttat gactgacaag atcgttgtcg ccgcgctgta taaattcgtt tccctgcctg
    45181 acttccacgc cctgcgcgag cctctgctgc aatgcatgct ggataacgct gtgaaaggca
    45241 ccttgctgct ggccgaagag ggcatcaacg gcaccgtctc cggctcgcgt gccgctatcg
    45301 atggcctgat ggcctggttt ggcctggacc cacgcctggc cgatatcgac cacaaggaat
    45361 cctattgcga tgagcagccg ttctatcgca ccaaggtcaa gctgaagaag gaaatcgtca
    45421 ccctgggcgt gcccggcgtt gacccgaacc agaaggtcgg cacttacgtc gaacccaagg
    45481 actggaacgc gctgatcagc gaccccgagg tgctgctgat cgatacgcgc aacgattacg
    45541 aagtatcgat tggtaccttc gaaggcgcgg tagacccgaa aaccaagtcg tttcgcgagt
    45601 ttcccgagta catcaaggcg cacttcgatc cggccaagca taagaaagtg gcgatgttct
    45661 gcaccggcgg tatccgctgc gaaaaggcct ccagctatat gctcggtgaa gggtttgagg
    45721 aggttttcca cctcaagggc ggcatcctca agtacctcga agaagtgccg caggaagaga
    45781 ccaagtggca gggcgactgc tttgttttcg ataaccgggt tactgtgcgt catgacctga
    45841 ctgaaggcga ttacgaccaa tgccacgcct gccgcacgcc ggtgtcggtg gaagatcgtc
    45901 agtccgagtt ctacacccca ggtgtcagtt gcccacattg ctgggattcg ctgccggaga
    45961 agacccgcgc cggtgcccgc gagcggcaac ggcagatcga actggccaag gcgcgcaatc
    46021 agccgcaccc gctgggctac aacccccgac aatcgcgcga ggcttaagca tgcaagcacg
    46081 cctgctctat gtgatggacc cgatgtgttc atggtgctgg ggctttgccc cggtactcga
    46141 gtccctcgcc gagcaggccg cggctgcagg cgttggcctg cagctagtgc tgggtggttt
    46201 gcgccgtgat ggcgtggcca tcgatgcggc ggcgcgggtg cgctacctgg gctactggca
    46261 ggcggtcaat gccagcaccg ggcagctgtt caacttcaac gagggtctgc ccgaggggtt
    46321 ggtgtacgac accgagccgg cctgccgtgc attggttacg gcgcgcagcc tggctcctga
    46381 gctggcctgg ccgctggcac agttgatcca gcgcgcgttc tatacccagg gcgtgaatgt
    46441 cactcgcgcc agtgtgctgg tggagttggc cgagcgcgcc gggattccgc gcatcgtgtt
    46501 tgccgaagct ttcgacagcg ccgcgcaacg cgccgccact gcagccgatg tcagctgggt
    46561 gcaggatctg ggcatcgctg gcttcccaac cctgcttgcc gaacgcaatg gccagatggc
    46621 cctgctgacc aacggctacc aaccgcttga ggtgctgcag ccgttgctgg cgcgctggct
    46681 ggagcgcgcc gcccatgcct gatcgcctga gctgggcgga aatccgccgg ctggccctgc
    46741 accacaaaaa agccctgatc ctggccaatc tggtcgccgt gctggccacc ctgtgcagcg
    46801 tgccgatccc gctgctgctg ccgctgctgg tcgacgaggt gttgctcaac gacggtgacg
    46861 ccgcgctcaa ggtgatggac aacttcctgc cggcgcactg ggaaaccgcc gccggctata
    46921 tcggcctgat gctggtgctg accctgtttc tgcgcggcgg tgcgctgatc ttcaacgtgc
    46981 tgcaggcgcg gctatttgcc cgcttgtcga aagacatcgt ctaccgcatc cgcattcgcc
    47041 tgatcgaacg gctcaagcgg atctcccttg gcgagtacga aagcctcggc ggcggcactg
    47101 tgaccacgca cctggttacc gacctcgata ccctggacaa gtttgtcggc gaaaccctta
    47161 gccgctttct ggtggccgtg ctgaccctgg ctggcacctc ggcgattctg atctggatgc
    47221 actggcagct ggcgctgctg atcctgttgt tcaaccctct ggtgatctac gccacggtgc
    47281 agttgggcaa acgggtcaaa cacctgaaga agctggaaaa cgacagcacc tcgcgcttta
    47341 cccaggcgct gaccgaaacc ctcgaagcga ttcaggaaat ccgtgccggc aaccgtcagg
    47401 gcttcttcct cggtcgcctc ggtggtcgtg ccaaggaagt gcgcgactac gccgtcgcct
    47461 cgcagtggaa gactgatgcg tcgaatcgcg ccagcggtct gctgttccag tttggcatcg
    47521 acgtgttccg cgcggcggcc atgctcaccg tgctgttctc ggatctgtcg atcggccaga
    47581 tgctcgcggt gttcagctac ctgtggttta tgatcggccc ggtcgagcaa ctgctcagct
    47641 tgcaatacgc cttctacgcc gccggtggtg cgttgacgcg gatcaacgag ttactggcgc
    47701 gtaaggacga gccgcagtac cccggccgcg ttgacccgtt caagggccgc gacaccgtcg
    47761 gtattgaggt cagcgggctg accttcgctt ataacgacga gccggtgctt aaccaactca
    47821 acctgagcat cgcgccaggc gagaaggtcg ccatcgtcgg cgccagcggc ggcggcaaga
    47881 gcaccctggt gcagctgctg ctcgggctgt atacgccgca ggctgggact atccgttttg
    47941 gtggcagcag ccaggaagaa atcggtctgg aaggcgtgcg tgaacacgtc gcggtggtgc
    48001 tgcagcatcc ggcgctgttc aacgacacgg tgcgcgccaa cctgtgtatg ggtcgtgaac
    48061 ggggcgatga cgcctgctgg cgcgccctgg aaattgccca gctggccgac accattcgcg
    48121 gcttgccgca aggcctggac agtattgttg gccgctccgg cgtgcgtctg tctggcggtc
    48181 agcgtcagcg cttggcaatt gcccgcatgg tgttggccga gccgaaagtg gtgatccttg
    48241 atgaagccac ctcggccctg gacgccgcca ccgaatacgc cttgcaccag gcgctcaatc
    48301 aattcctcca tggtcgtacc acgctgatca tcgctcaccg gctgagtgcg gttaagcagg
    48361 cggatcgagt gttggtattc gatggcggca gcatcgccga agacggcggc caccaagagt
    48421 tgattgccga aggtgggctg tacgccaagc tgtatgggca tctgcagcat tgacgatttt
    48481 ggtggatgaa aaaacgtcat ccaccctacg gtccttgtga gcacgacact tttttcgcgg
    48541 gcatggcccg ctcctacggg taaacccccg actgtaggag cgggccatgc ccgcgatttg
    48601 ctgtgggttt caacataggt gcgagcccct gcggcctcgc acctggcagt taaatcgacc
    48661 gctttgtaaa tttatcgtta cgcatgccga gtgctttcgg ccagcattcc cgctgtttct
    48721 ctagttgtat taaaaacttg ccgaaagcct tggccaagcc tggggcaggg cgctctgggc
    48781 cggcttgtta gcgggcctgc gctcggttgt tatgaggtca atcccgctga cgcagcggct
    48841 gatcaaaaca acaacgagga ggcgcaatga acgccgtgaa caagatcgaa cagcacaacc
    48901 ctatcggtac cgacggcttc gagttcgttg aatacaccgc gcctaccccg gaaggcatcc
    48961 agcagctgcg tgagctgttc actgccatgg gcttcaccga aacggccaag caccgctcga
    49021 aagaggtctg gctgttccag cagaacgaca tcaacttcgt gcttaacggc agccctactg
    49081 ggcacgtgcg cgagttcggt ctgaagcacg gcccaagtgc ctgcgccatg gcattccgtg
    49141 tgcagaacgc cgcccaggcc gccgcctatg tggagtccca gggcgccaaa ctggtaggca
    49201 gccacgccaa ctttggcgag ctgaatattc cctgcgtgga aggtatcggc ggctccttgc
    49261 tgtatctggt ggaccgttac ggcgacaaga gcatctacga cgtcgacttc gagtacatcg
    49321 aaggccgcac gccgaacgac aattcggtcg gcctgaaaga gctggatcac ctgacccaca
    49381 acgtcaaacg tgggcagatg gacgtgtggt cgggcttcta cgagcgcatc gccaacttcc
    49441 gtgagatccg ctatttcgat atcgaaggca agctcaccgg cctgctgtca cgcgccatga
    49501 ccgcgccgtg cggcaagatt cgcatcccga tcaacgaatc gtccgacgac aagtcgcaga
    49561 ttgaggaatt tatccgcgag taccagggtg agggcatcca gcatatcgcc ctgagtaccg
    49621 aggatatcta cgccaccgtg cgccagctgc gcgccaatgg cgtggatttt atggtcaccc
    49681 ccggcactta ctacgacaag gtcgataccc gtgtggccgg gcatggcgag ccgagcgatg
    49741 tgctgcgtga gctgaatatc ctgatcgacg gagctccggg cgtcggtacg tcgggcgatg
    49801 acggcatcct gctgcagatt ttcaccaata ctgtgattgg cccgatcttc ttcgagatca
    49861 tccagcgcaa gggcaaccag ggctttggcg agggcaactt caaggcgctg ttcgagtcca
    49921 tcgaggaaga tcagatcaaa cgcggcgtga tctccgagga gtgaagccat gagccgtaac
    49981 tggatcagtt ttcccctgcg tgaaggcgag tgttcgcgcc aggcgcactg cgactttcct
    50041 gagggcactt atgagcgcga gatgggccgc gagggctttt tcggccctac ggcgcacctg
    50101 caccacaagc atccaccgac cggctggatc gactggcagg ggccgctgcg cccccacgcg
    50161 ttcaacttca atgatatttc cagcgagcgt gattgtccgc tggccgcacc gctgaccctg
    50221 cataacagcg atgtgaagct gcgcgtgtgg aaaacccacg gcgccatgcg tcatctggtg
    50281 cgcaacgccg atggcgatga cctgctgttt attcatgagg gagcagggca cttctattgc
    50341 gatttcggtc atttgaccta ccgcgacggc gattacctgc tgattccgcg cggcaccgcc
    50401 tggcgcatcg aggccagtga accgaccttt atgctgctga tcgagagcac cgatggtgcc
    50461 tatcagctgc cggacaaggg cctgcttggg ccgcaggcga tcttcgatcc ggcggtgctc
    50521 gatcatccgc gcatcgatga tgccttcaag gcgcagcagg atgaaaacac ctggcagatc
    50581 cgcatcaagc ggcgtgccca gatcagcacc gtgacttacc cgtacaaccc gctggatgtg
    50641 gtcggctggc atggcgacaa caccgtggtg cgtctcaact ggcgtgatat ccgcccgctg
    50701 atgagccacc gctatcacct gccgccatcg gcgcacacca cctttgtcgc caatggtttt
    50761 gtgatctgca cctttacccc gcgcccagtg gaaagcgacc ccggcgcgct gaaggtgccg
    50821 ttctatcaca ataacgatga ctacgacgaa gtgctgttct accaccgtgg caacttcttc
    50881 agtcgcgaca atatcgagca gggcatggtc accctgcacc cgtgcggctt cccccacggc
    50941 ccgcacccca aggcgctgaa aaaggcgcag accgatccgg cgacctttat cgatgaagtg
    51001 gcggtgatga tcgatacccg ccgcgccctg gaagtcggcg aagccgccgc cggcgtggat
    51061 gttcctgaat atgtgaattc ctggcgtgcg ccgggcaagg aatcctgatg aaactcgcat
    51121 cactgaatca aggccgtgac ggcgtgctcg ttgtggtatc gcgcgaccta agtcgcgcca
    51181 ccgaagtccc ggcgattgcc gttaccctgc agggagcgct ggataactgg gcggtggcca
    51241 agcccaagct ggaggcggtg tatcagcgtt tgaacgatgg cctggaagag ggagcgtttg
    51301 ccttcgatca ggcggcctgc cacagcccat tgccgcgtgc ctatcactgg gccgatggca
    51361 gcgcctacgt caatcatgtg gagctggtgc gcaaggcgcg tggcgccgaa atcccggaaa
    51421 gcttctggca tgacccgctg atgtaccagg gcggtgccga ctgctttatc ccaccgcaca
    51481 gcccgatcca gatggccgac gaggcctggg gcatcgacct ggaagccgag atcgcggtga
    51541 tcaccgatga cgtgccgatg ggggcaaccg ccgccgaggc cgcctcgcat atccagctgc
    51601 tgatgctggt caacgacgtg tcgctgcgta acctgattcc cggcgaactg gccaagggct
    51661 tcggtttcta ccagagcaag ccctcatcca gcttctcgcc ggtggcgatt accccggatg
    51721 agctgggcga tacctggcgc gatggcaagg tgcatcggcc gctggtttcg catatcaacg
    51781 gagcgctgtt cggtcagccg gatgccggcg tggacatgac cttcaacttc ccaaccctgg
    51841 tggctcacgc cgccaagacc cggccgctgg gctgcggcac catcatcggt tccggcaccg
    51901 tgtcgaacta cgaccgtagc gcgggttctt cctgcctggc agagaagcgc atgctggaaa
    51961 tcatcgagag cggcgaggcg aaaaccccgt tcctcaagtt cggtgaccgg gtgcgcatcg
    52021 agatgttcga cgccgccggc catagccttt ttggcgccat cgatcaggcg gtagagcgct
    52081 atgagcactg agttgacgct ctacggttac tggcgctcca gcgccgtcta ccgggtgcgc
    52141 atcgcgttga acctcaaggg cctggcttat caacaagtgc cggtgcacct ggtcaaggac
    52201 ggcggccagc agcattcggc cgaatatcag gcgctgaatc cgcaggagct gctgcccttg
    52261 ttggtggata agggcaacgg cggggtgcgt atcgcccagt cgctggcgat tctcgaatac
    52321 atcgacgagg tgtttccggc gcaggcactg ctgcccagca atccggcgca gcgcgctcag
    52381 gtgcgcgccc tggctctgca tatcgcctgc gacctgcatc cgctgaacaa cctgcgggt
//
LOCUS       DASRUM010000159        10088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_592882, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000159 DASRUM010000000
VERSION     DASRUM010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10088
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592882"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..104)
                     /locus_tag="VFF22_10525"
     CDS             complement(<1..104)
                     /locus_tag="VFF22_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HZX17285.1"
                     /translation="MIDSDGFRPNVGIILTNDVGQVLWARRINQDAWQ"
     gene            260..913
                     /locus_tag="VFF22_10530"
     CDS             260..913
                     /locus_tag="VFF22_10530"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZX17286.1"
                     /translation="MRLALFDLDYTLLGGDSDHAWGDYLCERGILDGIAYKARNDDFY
                     QDYLAGRLNITDYLNFSLEILGRTEMAQLEQWHREFMRDCVEPIILTKGEALLAEHRE
                     AGDKLLIITATNRFVTAPIAARLGVDTLLATECEMADGRYTGRTTDVPCFKEGKVTRL
                     NRWLAESGLNLNDSYFYSDSMNDLPLLEQVTHPFAVDPDPNLRAEAEKRGWPVISLR"
     gene            complement(970..1410)
                     /locus_tag="VFF22_10535"
     CDS             complement(970..1410)
                     /locus_tag="VFF22_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HZX17287.1"
                     /translation="MEHYPLLKMLHMLPGILLLVGVLVHLFMLWKASRGGDAAILQRK
                     LKRTRLISMPVLGLLALSLPVTGWLLVDLIGLPLSQTWLLLSSVLFGVLMVAGLLLAG
                     RLSAWQALNGAPASKRLLGFCAVYAGLIVLLLLVIMGLMGAKPA"
     gene            complement(1437..3008)
                     /gene="ilvA"
                     /locus_tag="VFF22_10540"
     CDS             complement(1437..3008)
                     /gene="ilvA"
                     /locus_tag="VFF22_10540"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HZX17288.1"
                     /translation="MRSPGVDSRTPSKAACDHAMLEQYVKKILTSRVYDVAVETPLQA
                     ANQLSERLGNQILLKREDLQPVYSFKIRGAYNKLAQLSAEEQARGVVTASAGNHAQGL
                     ALAAKVLGVKATIVMPKTTPEIKVQGVRSRGGKVVLHGDSFPEALAYSLKLVDEKGYI
                     YIHPYDDPHTIAGQGTVAMEILRQQPGAIDAIFVPVGGGGLIAGIAAYVKYLRPDIKV
                     IGVEPDDSNCLQAAMAAGERVVLPQVGLFADGVAVAQIGEHTFAVCKDYVDEVITVST
                     DEICAAIKDIYDDTRSITEPAGALSVAGIKKYVEREGCSGQVLVGIDSGANVNFDRLR
                     HVAERAELGEKREAIIAVTIPEQPGSFKAFCEAIGKRQITEFNYRYHEDKEAHIFVGV
                     QTHPESDPRAALVQGLRDQGFPVLDLTDNELAKLHIRHMVGGHAAAVPDECVYRFEFP
                     ERPGALFNFLQKLGGRWNISMFHYRNHGAADGRVVAGLQVPQAERHLVPAALEAIGYP
                     YWDESDNPAYKLFLG"
     gene            3109..3783
                     /gene="rpiA"
                     /locus_tag="VFF22_10545"
     CDS             3109..3783
                     /gene="rpiA"
                     /locus_tag="VFF22_10545"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016979019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HZX17289.1"
                     /translation="MNQDQLKQAVAQAAVDFILPKLDAKSVIGVGTGSTANCFIDALA
                     KHKMEFDGAVASSEATAARLKGHGIPVYELNTVSDLEFYVDGADESDEHLNLIKGGGA
                     ALTREKIVAAVAQTFICIADASKLVPVLGAFPLPVEVIPMARSHVARQLVKLGGDPVY
                     REGVVTDNGNIILDVFNLSITNPAELESQINAIVGVVTNGLFAARPADLLLLGTADGV
                     KTLKRA"
     gene            3901..5685
                     /locus_tag="VFF22_10550"
     CDS             3901..5685
                     /locus_tag="VFF22_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HZX17290.1"
                     /translation="MSAFVSSAHDRQLDLGDLLRELVAQGRTDQDNAEQCLAIRRSAV
                     NNKQHPLEFLASQQLDDLKNPGKKLELEGLTSWLAEQCGQPFLRIDPLKINVAAITPL
                     MSYAFAQRHKILAVAVDNDSVTIASSEPLVNSWEANLTHVLKRPIKRVVASPADIQRF
                     TVEFYRLAKSVSGASAGDMKVSGAGNFEQLLNLGAQDQEPDANDAHIVNIVDWLFQYA
                     FTQRASDIHIEPRREQGSVRFRIDGVLHTVYQFPPQVTMAIVSRLKSLGRMNVAEKRK
                     PQDGRVKTKTPDGGEVELRLSTLPTAFGEKMVMRIFDPEVLLKSFDQLGFSNDDLRRW
                     EYMTSQPNGIILVTGPTGSGKTTTLYTTLKKLATSEVNLCTIEDPIEMIEPAFNQMQV
                     QSNIDLTFASGVRALMRQDPDIIMVGEIRDLETAEMAIQAALTGHLVLSTLHTNDAPS
                     AITRLLELGVPHYLLKATLLGVMAQRLVRTLCPHCKVPVDIVDEDWQALTKPWSSPAP
                     TGAHKAVGCLECRDTGYRGRAGVYEIMQLNDGIKPLITADTDLIALRRHAFKEGMRSL
                     RLSGAQKVAAGLTTIEEVLRVTPQSEQK"
     gene            complement(5701..6369)
                     /locus_tag="VFF22_10555"
     CDS             complement(5701..6369)
                     /locus_tag="VFF22_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019821829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HZX17291.1"
                     /translation="MPFSATAAEQDGLLKDLTRIAPGLNQQALRHALAAMQCAVNHGA
                     DPARRLAVIDYSRPSTERRLWIFDLQRKRLLLRDFVAHGRKSGDNFAEVFSNSLGSHQ
                     TSLGLFRTAESYSGKHGYSLRMDGLEPGLNDLARERAIVIHGADYVNPLWVAKQGRIG
                     RSLGCPAVRPEVARMVVDQLKGGQFLFAWHSNQRLMQASTYLECKPQRVASILARQQR
                     LPRS"
     gene            6529..8115
                     /locus_tag="VFF22_10560"
     CDS             6529..8115
                     /locus_tag="VFF22_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009044553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZX17292.1"
                     /translation="MFKKRTLYLSAALLTLPLVANATDALFATSADLRPLLAQLTQRC
                     SPLPTSLDENAQRLLQAFYAQRDGQPAWQNSEQLLQLREQLAQLADDGLQPNDYRLPS
                     LVAESFTEQRDCAELLTSHSYLQALLHLRRGRLLQQRLEPLWQAPGQTNPDPQLATLS
                     LALLHVHMPAAAFVSARPATVQYQRLRNAYAQQRLQPLQEWATLASGPQLKPGGKDAR
                     LPELRERLIAQGYLAAPLEDLGSTYDPATQSALESFQRDHGLNPDGILGPASLSELNI
                     SPQMRREQLRANLERLRWLADTMDEAEVLINVAAAEIQIRRNNQLLWRTRTQVGRAER
                     QTPLLASSIVRLTLNPTWTVPPTILREDKLPAIRDDIGYLESQQMSVLDRDGNLLDPH
                     SIDWDNPGPIRLRQSAGSHNPLGRVAVRFDNPFAVYLHDTPSQQLFSKAPRAFSSGCI
                     RVEAVDTLLAWLLTPDELSSVQTRIASGETQQYRLQHPAPLLIAYWTVEATKDGALRY
                     YPDIYARDARLIKALAPSVH"
     gene            8239..9159
                     /locus_tag="VFF22_10565"
     CDS             8239..9159
                     /locus_tag="VFF22_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017845099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZX17293.1"
                     /translation="MEIGSVIFLFVGLAVAIVFMGFKVVPQGFEWTVERFGRYTNTLK
                     PGLNIIVPVMDKVGRKLNVMENVLDIPPQEVITADNATVQIDAICFFQIINSAQAAYE
                     VNNLEHAIRNLVMTNIRTVLGSMELDAMLGQRDAINEKLLRTVDEATAPWGIKITRIE
                     IKDISPPADLMAAMSGQMKAERIKRAQILEAEGLRAAAILTAEGQKQGDILKAEGQRQ
                     AAFLEAEARERAAQAEAEATRMVSEAIAQGNVQAVNYFVAQKYIEALGQLASANNSKV
                     ILMPLEASQVIGAVGGISEIVKATFGEKKG"
     gene            9163..9618
                     /locus_tag="VFF22_10570"
     CDS             9163..9618
                     /locus_tag="VFF22_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010450675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HZX17294.1"
                     /translation="MWDFLQHLSYWNWLAFGTLLLILEVFGAGGYLLWIGLAAASVGL
                     LTFIFPDLPWAAQFILFGLLSILTAVFWWQRQRSAAKPSDQPGLNQRGSEFVGRTFVL
                     HEAINGGRGKIKAGDSLWLVCGPELPAGTSVRVMGQEGVVLQVVAAQPD"
     gene            complement(9680..10069)
                     /gene="gcvH"
                     /locus_tag="VFF22_10575"
     CDS             complement(9680..10069)
                     /gene="gcvH"
                     /locus_tag="VFF22_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013984409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HZX17295.1"
                     /translation="MSNIPADLRYAASHEWARLEADGSVTVGISDHAQEALGDVVFIE
                     LPEVGKSLNAGQEAGVVESVKAASDIYAPISGEVIAINEGLADSPESVNSDPYGSWFF
                     KLKPSDVSELDKLLDAAAYKATADSDA"
BASE COUNT         2146 a         3306 c         2844 g         1792 t
ORIGIN      
        1 aactgccaag cgtcctggtt tatccgacgc gcccacagca cctggccgac atcattggtc
       61 agaatgatgc cgacattcgg gcgaaaacca tcggaatcga tcacggcaca caacctcgta
      121 aacgcctgaa tttaagacat gcgggcattg ttccacaaag gccgcggcag cagcaacgcg
      181 agcggcccgt gtaataggaa agccttataa aagccgttat tctggccgcc tttcgtggct
      241 ggcaatcaaa ggggctcccg tgcgtttggc tttattcgat ctcgactaca ccttgctcgg
      301 cggcgacagc gatcacgcct ggggcgacta cctgtgtgaa cgcggcatcc tcgacggcat
      361 cgcctacaaa gcgcgcaacg atgacttcta tcaggactac ctggccggcc gcctgaacat
      421 caccgactac ctgaatttca gcctggaaat cctcggccgc acggaaatgg cccagctaga
      481 gcagtggcac cgcgagttta tgcgcgactg cgtcgagccg atcatcctta ccaaaggtga
      541 agcgctgctc gccgagcacc gcgaagccgg cgacaagctg ctgatcatca ccgccaccaa
      601 ccgcttcgtc accgcgccga ttgctgcgcg ccttggggtc gacaccctgc tggccaccga
      661 atgtgaaatg gccgacggcc gctacaccgg acgcaccacc gacgtgccgt gcttcaagga
      721 aggcaaggtc acccgcctta accgctggtt agcggaaagc ggccttaacc tcaacgacag
      781 ttacttctac agtgactcaa tgaacgacct gcccctgctg gagcaagtca ctcatccctt
      841 cgccgtcgac cccgatccaa acctgcgcgc cgaagcggaa aagcgcggct ggccggttat
      901 atcgctgcgc taaatccgcc gcaaataaaa acgccgcctg atcaggcggc gtttttgttt
      961 gggtacagat caggccggct tggcccccat caggcccatg attaccagca acagcagcac
     1021 aatcagccct gcatacaccg cgcagaaacc caacaggcgt ttagatgccg gagctccatt
     1081 cagagcttgc caggcgctca ggcggcccgc cagcagcaac ccggcaacca tcagcacgcc
     1141 aaacagcacg ctgctgagca gcagccaggt ttggctcagc ggcaaaccga taaggtcaac
     1201 cagcaaccag ccggttaccg gcagactcag ggccagcagg ccgagaaccg gcatgctgat
     1261 caagcgggtg cgtttaagct tgcgctgcaa gatggcggca tcaccaccac gtgaggcctt
     1321 ccatagcata aacagatgca ccagcacccc aaccagcaag aggatgccgg gcagcatgtg
     1381 cagcattttc agcaatgggt aatgttccat tgtttcagtc ctaccgggtt tccggcttat
     1441 ccaagaaaga gtttgtacgc cggattgtcg ctctcatccc aatagggata gccgatggct
     1501 tccagcgccg ccggcacgag atgacgctcc gcttgcggca cttgcagacc cgcaacaacg
     1561 cggccatcgg ccgcgccgtg attgcgatag tggaacatcg agatgttcca gcgcccacct
     1621 aatttctgta agaaattgaa caatgcaccc ggacgctcag gaaactcgaa gcggtaaacg
     1681 cactcatccg gcaccgctgc cgcatgaccg ccgaccatat gacggatatg caacttggcc
     1741 agttcgttgt cagtcaggtc caataccggg aagccctggt cgcgcagacc ctgaaccagc
     1801 gcggcacgcg ggtcgctttc cggatgggtt tgcacgccga caaagatgtg cgcctcctta
     1861 tcttcgtgat agcggtagtt gaactcggtg atctgccgct tgccgatggc ttcgcagaac
     1921 gccttgaagc tgcccggttg ctcggggatg gtcacggcga tgatcgcttc acgcttctcg
     1981 cccagttcgg cgcgttcagc aacgtggcgc agacggtcga agttgacgtt ggcacccgag
     2041 tcgatgccca ccagcacctg accgctgcaa ccttcacgct ccacgtactt tttgatcccg
     2101 gccacgctca gcgcaccggc cggctcggtg attgagcggg tgtcgtcgta aatgtccttg
     2161 atcgccgcgc agatctcgtc ggtgctgacg gtgatcactt cgtcgacata gtccttgcag
     2221 acggcaaagg tgtgctcgcc gatttgcgcc acggccacgc catcggcaaa cagcccgacc
     2281 tgcggcaata ccacacgctc ccctgcagcc atggcagctt gcaggcagtt ggagtcgtcc
     2341 ggttcaacac cgataacctt gatgtccgga cgcaggtatt tcacataggc cgcgataccg
     2401 gcgatcaggc caccgccgcc aaccggcacg aagatcgcgt cgatagcgcc tggctgctga
     2461 cgcagtatct ccatggccac ggtgccctgg ccagcgatgg tgtgtggatc atcgtaagga
     2521 tggatataga tgtagccctt ttcatccacc aatttcagcg aataggccag cgcctcgggg
     2581 aagctgtcgc cgtgcagcac caccttgccg ccacgggaac gcacgccctg aactttgatc
     2641 tccggcgtgg ttttgggcat cacaatggtg gccttgaccc ccaacacctt ggccgccagt
     2701 gccaggccct gggcatggtt gccggccgag gcggtgacca caccacgggc ctgctcttca
     2761 gccgagagct gcgccaattt gttgtaggct ccacgaatct tgaacgagta caccggctgc
     2821 aggtcttcgc gcttgagcag aatctggtta cccaggcgct cggaaagctg gttggcggct
     2881 tgcagcgggg tttccaccgc cacgtcatag acgcgcgagg tgaggatctt cttgacgtac
     2941 tgttcgagca tggcgtgatc gcaggcggct ttcgagggag tccgcgagtc taccccaggg
     3001 ctgcgcagat cgaccacata acgcgcaggg ttttcccgcc tcaggtccgc tggcggccca
     3061 gccgcgctat aattcgcgcc tttcctcact cccacgccag gcaccatgat gaaccaggat
     3121 caactcaagc aagccgtcgc ccaggctgcc gtcgacttta ttctccccaa actcgacgcc
     3181 aagagtgtga tcggagtcgg cactggctcc accgccaact gttttatcga tgccctggcc
     3241 aagcacaaga tggaatttga cggcgccgtc gccagctccg aagccaccgc cgcacgcctc
     3301 aaaggccacg gcattccggt gtatgaactg aatacggtca gcgacctgga gttctatgtc
     3361 gacggcgccg atgagagcga cgagcacctc aacctgatca agggcggcgg cgcagccctg
     3421 acccgcgaga agatcgttgc tgccgtagct cagaccttta tctgcatcgc cgacgccagc
     3481 aagctggtac cggtactggg tgccttcccg ctgccggtgg aagtgatccc gatggcgcgc
     3541 agccacgtcg cccgccaact ggtcaaactc ggcggcgacc ctgtgtaccg cgaaggcgtg
     3601 gtgaccgata acggcaatat catcctcgat gtgttcaacc tgtcgatcac caacccggcc
     3661 gagctggaaa gccagatcaa cgccatcgtc ggcgtggtca ccaatggcct gttcgctgcc
     3721 cgcccggccg atctgctgct gctgggcaca gccgacggag tgaagacgct taaacgtgcc
     3781 tgacaccatg agaggcaaga gctagcggct gtgcaaaaat ggatcaagtc ggcagacaac
     3841 ccatgtcctt ggcgtcatgc tgccggcagt gtttagtgtt caggatcgtc agggaacttt
     3901 atgtccgcat ttgtctcgtc cgcccatgat cgccagcttg accttggcga cctgctgcgt
     3961 gagctagtcg cccaaggccg aaccgaccaa gacaatgccg aacagtgcct ggcgatccgc
     4021 cgcagcgccg taaacaataa gcagcacccg ctggaatttc ttgcctccca gcaactcgat
     4081 gacctgaaaa atccaggcaa aaagcttgag ctggaaggat tgaccagttg gctggcggag
     4141 caatgcggcc agccctttct gcgtatcgat ccactgaaga tcaatgtcgc ggccatcacc
     4201 ccgctgatgt cctacgcctt cgcccaacgc cacaagattc ttgccgtggc ggtggacaat
     4261 gacagcgtga ccatcgccag cagcgagcct ctggtaaaca gctgggaagc caacctgacc
     4321 cacgtactca agcgcccgat caagcgcgtg gtggccagcc ctgcggatat ccaacgcttt
     4381 accgtggagt tctaccgcct ggccaagtcg gtcagcggcg cctcggccgg cgacatgaag
     4441 gtcagcggcg cgggcaactt cgaacaactg ctcaacctcg gcgctcagga tcaggagccc
     4501 gacgccaacg acgcgcatat cgtcaatatc gttgactggt tgttccaata cgccttcacc
     4561 cagcgcgcca gcgatattca tattgagccg cgccgcgagc agggcagcgt gcgctttcgt
     4621 atcgacggcg tgctgcacac cgtctaccaa ttcccgcccc aggtaaccat ggccatcgtc
     4681 agccgcctga aaagcctggg ccggatgaat gttgcagaaa agcgcaaacc ccaggacggc
     4741 cgggtcaaga ccaagacccc agacggcggc gaagtggagc tgcgcctgtc gaccctgccc
     4801 accgcgtttg gcgaaaagat ggtgatgcgg attttcgacc cggaagtgtt actgaaaagc
     4861 ttcgaccaat tgggtttctc caacgatgac ctgcgccgct gggaatacat gaccagtcag
     4921 cccaacggca tcattctggt cacaggcccc accggttcgg ggaaaactac cacgctgtac
     4981 accacgctga agaagctggc gactagcgag gtcaacctgt gcaccatcga agacccaatc
     5041 gagatgatcg agccggcctt taaccaaatg caggtgcaga gcaatatcga cctgaccttc
     5101 gccagcggcg tgcgcgcgct gatgcgccag gacccggaca tcatcatggt cggcgagatc
     5161 cgcgacctgg aaaccgccga gatggcgatc caggcggcgc tgactggcca cctagtactc
     5221 tcgaccctgc acaccaacga cgcgccaagc gccatcaccc gcctgctcga gctcggtgtg
     5281 ccgcactatc tgctcaaagc caccctgctc ggggtcatgg cccagcgcct agtacgcacc
     5341 ctgtgcccgc actgcaaggt gccggtggat attgtcgacg aagactggca agcgctgacc
     5401 aaaccctgga gctcaccggc cccgaccggc gcgcacaagg ccgttggctg tttggagtgc
     5461 cgcgacaccg gctaccgcgg ccgcgccgga gtgtacgaga tcatgcagtt gaacgacggc
     5521 atcaagccgc tgatcactgc cgacaccgac ctgattgccc tgcgccggca tgccttcaag
     5581 gaaggcatgc gcagcctgcg cctgtccggc gcgcaaaaag tcgcagccgg cctgaccacc
     5641 attgaggaag tgctgcgggt caccccgcag agcgagcaga aatagccttc ccgcgaaatt
     5701 tcagctgcga ggtaaccgct gctggcgcgc caggatgctc gccacccgct gcggcttgca
     5761 ctccagatag gtagaggcct gcatcaagcg ttgattggag tgccaggcga aaagaaactg
     5821 cccgccctta agctgatcga ccaccatccg tgccacttcc ggccgcaccg ctgggcaacc
     5881 cagactgcgg ccgatacgac cctgtttcgc tacccagagc ggattcacat aatcggcacc
     5941 atgaatgacg atagcgcgct cacgggccag atcatttagc ccaggctcca ggccgtccat
     6001 acgcagggaa tagccatgct tgccactgta gctttccgcg gtgcggaaca agcccagact
     6061 ggtctgatgg ctacccagac tattggaaaa cacctcggca aagttatccc cggatttgcg
     6121 cccatgggcg acaaagtcgc gcaacagcaa acgctttcgc tgcagatcga aaatccataa
     6181 gcgtcgctcg gtagacggcc gcgaataatc gatcaccgcc agacggcgcg cagggtcggc
     6241 gccatgattg accgcacact gcatagccgc cagggcatgc cgcagtgcct gttgattaag
     6301 cccgggagca atccgcgtga gatccttgag cagcccatcc tgctcggctg cggtcgccga
     6361 aaacggcata taagcaagcc acaacccgat taggcagaac ctgccaagac gtccaaacat
     6421 tgcgcaaata cctctagtac cttcggacgt cctgtcacaa gatccaacca tcgcccgcct
     6481 atactggctg cggccaagtt gttttagccc atggaatgga gcagttgatt gttcaaaaaa
     6541 cgcacactct acctgagcgc tgccctgctc accctgccat tggtcgccaa cgccaccgat
     6601 gcgctcttcg ccaccagcgc cgatctgcgc ccattgctgg cccagttgac gcagcgctgc
     6661 agcccattac cgacaagcct agacgagaat gcccagcgcc tactgcaggc tttttatgcc
     6721 caacgcgacg gccaacccgc ctggcagaac agcgaacagc tgctgcaatt gcgcgagcag
     6781 ctggcccaat tggccgatga cggtctgcag ccaaacgact atcggctgcc cagtttggtt
     6841 gcggaaagct tcaccgagca gcgcgactgc gcggaactgc tgactagcca cagctacctg
     6901 caagccctgc tgcacttgcg ccgcggccgc ttgctgcaac agcgcctgga accgctgtgg
     6961 caagcgccag ggcaaaccaa cccggacccg caactggcga ccctctcact ggcgttgctg
     7021 catgtgcata tgccggccgc ggcctttgtc agtgcgcggc cggccaccgt gcaataccag
     7081 cggctgcgta acgcttacgc gcaacaacgc ctgcagccgc tgcaggagtg ggcgacgctg
     7141 gccagcgggc cacagctcaa acccggcggt aaggatgcgc ggctgcccga attgcgcgaa
     7201 cgcctgatcg cccagggcta cctggctgca ccgctagaag atctgggcag cacttatgat
     7261 ccagccacgc aaagcgccct agagagtttt cagcgcgatc atggcctcaa cccggatggc
     7321 atcctcggcc ccgccagcct gagcgagctg aatatcagcc cacagatgcg ccgcgagcaa
     7381 ctgcgcgcca acctcgaacg cctgcgctgg ctggccgaca ccatggatga ggccgaagtg
     7441 ctgatcaatg tcgcggccgc cgaaatccag atacggcgca ataaccagct gctgtggcgc
     7501 acccgcaccc aggtcggccg cgccgaacgg caaacgccgc tgctcgcctc aagcatcgtg
     7561 cggctgaccc tcaatccaac ctggaccgtg ccgccaacca tcctgcgcga agacaaactg
     7621 ccagccattc gcgacgacat cggctacctg gaaagccagc agatgagcgt gctcgaccgc
     7681 gacggcaacc tgctcgaccc tcacagcatc gattgggata accccggacc gatccgcctg
     7741 cgccagtcgg ccggcagcca taacccactc ggccgggtcg ccgtgcgctt cgataatcca
     7801 ttcgcggtat atctgcacga cacccccagc cagcagctgt tcagcaaggc gccgcgtgcg
     7861 ttcagctcgg gctgcatacg ggtcgaggcc gtcgacaccc tgctcgcctg gctgctcacc
     7921 ccggatgagc tgagcagcgt gcaaacgcgc atcgccagcg gcgaaacgca gcaataccgt
     7981 ctgcaacatc cggcgccgct gctgatcgcc tactggacgg tcgaggccac caaggacggg
     8041 gccctgcgct actacccgga catctatgcg cgtgacgccc gcctgatcaa agccctggcg
     8101 ccaagcgtgc actgatcggc agccatgacc gttgatcggc acactctgca acggcatcga
     8161 cagccgcgcc aacgctaact gtgcttagat acctgaacag ccaaaccaca gactaaaacc
     8221 gccaaggaac tacaccacat ggaaatcggc agcgtcattt ttctcttcgt cggcctcgcc
     8281 gtcgccatcg tctttatggg cttcaaggtt gtaccgcagg gcttcgaatg gacagtggag
     8341 cgcttcggcc gctacaccaa cacgctcaag ccgggcctga acatcatcgt gccggtgatg
     8401 gacaaggttg gccgcaagct caacgtgatg gaaaacgtgc tggatattcc gccgcaggaa
     8461 gtgattaccg ccgataacgc caccgtgcag atcgatgcca tctgcttctt ccagatcatc
     8521 aactcggccc aggcggcata cgaggtaaac aacctcgaac acgccatccg caacctggtg
     8581 atgaccaata ttcgtaccgt gctcggctcc atggaactgg acgccatgct cggccagcgc
     8641 gatgcgatca acgaaaagct gctgcgtacc gtcgatgaag caaccgcgcc gtggggcatc
     8701 aagatcaccc gtatcgagat caaggacatc agcccgcccg ccgacctgat ggccgccatg
     8761 tccgggcaga tgaaagccga acggatcaaa cgcgcacaga ttctcgaagc cgaaggcctg
     8821 cgcgccgccg ccattctgac tgccgaaggg cagaaacagg gcgatatcct caaggccgaa
     8881 ggccaacgcc aggccgcttt cctcgaagcc gaagcgcgcg aacgtgccgc ccaggcagaa
     8941 gccgaggcca cgcgcatggt ttccgaagcc atcgcccaag gtaacgtgca ggcggtcaac
     9001 tacttcgtcg cgcagaaata catcgaagcc cttggccaac tggccagcgc caacaacagc
     9061 aaggtcatcc tgatgccgct ggaagccagc caggtgatcg gtgcggtcgg cggcatcagc
     9121 gaaattgtca aagccacctt cggcgagaag aagggttaaa ccatgtggga ctttttacag
     9181 cacctgtcgt actggaactg gctggcgttc ggcaccctgc tgctgatcct cgaagtgttc
     9241 ggtgccggtg gctatctgct gtggatcggc ctggccgccg ccagcgtcgg cctgctgacc
     9301 tttatcttcc cggacctgcc gtgggctgcg cagttcatcc tgttcggcct gctctcgatc
     9361 ctcacggcag tgttctggtg gcagcgccag cgtagtgcag ccaagccttc cgaccagccg
     9421 gggctgaacc agcgcggcag cgaattcgtc ggccgcacct tcgtcctgca cgaagcgatc
     9481 aatggcggac gcggcaagat caaggccggc gattcgctgt ggcttgtctg cggtccggaa
     9541 ctgccggccg gcacatctgt acgtgtgatg ggccaggagg gcgtggtgtt gcaagtcgtt
     9601 gccgcacaac cagactgatc gccaacgctg taaacgacaa cgccccgcat tgcggggcgt
     9661 tgtcgtttaa ccatgaacct tatgcgtcgc tgtcggcagt ggccttatag gcagcagcat
     9721 cgagcagctt gtccagctcg ctaacatcgc ttggcttgag cttgaagaac cagctgccgt
     9781 acggatcgct gttgacgctt tccgggctgt cggccaggcc ttcgttgatg gcgatcactt
     9841 caccggagat cggtgcatag atgtcggacg cggccttgac cgactccacc accccagctt
     9901 cctggccggc attcaggctc ttgccgactt ctggcagctc gataaacacc acatcaccca
     9961 gggcttcctg cgcgtgatcg gaaatgccta cggtaacgct gccatcggct tccaggcgcg
    10021 cccactcgtg gctggcggcg taacgcagat cggcggggat attgctcatg tgtcgttcct
    10081 catctgac
//
LOCUS       DASRUM010000160         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_244589, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000160 DASRUM010000000
VERSION     DASRUM010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244589"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..778
                     /locus_tag="VFF22_10580"
     CDS             335..778
                     /locus_tag="VFF22_10580"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX17296.1"
                     /translation="MRQTELHLTDEDRELIESYRAKGLHHAREVNRAHVLSALDRGIP
                     EAQIMAVLGVGRTVIWRTRVAYLEGGVEYAVQDVERPGKPRKYDTDVEAQVAALACSE
                     PPAGAQRWTLALLEQAAQAQAGVGPISRETVRRLLKKTASSRGAS"
     gene            778..1437
                     /locus_tag="VFF22_10585"
     CDS             778..1437
                     /locus_tag="VFF22_10585"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HZX17297.1"
                     /translation="MWCIGRLTEEYRQRMYDLLDLYARPFRAKEPVICLDEKSKQLLK
                     DSRAPLPIRPGMPVRQDYEYVRAGTCNLFVAVEPKGGRRTVVVTDHRAKTDFVAFVQY
                     LLEQVYGTARRVHLVMDNLNTHFRKCFEDVLGVKEAKALLRRVAFHYTPKHASWLNMA
                     EIEIGILDRQCLDRRLPDRTTLVAEVDAWQQRRNAEQRGIAWSFTRQDADQKMARHYV
                     S"
     gene            complement(1454..>2032)
                     /locus_tag="VFF22_10590"
     CDS             complement(1454..>2032)
                     /locus_tag="VFF22_10590"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZX17298.1"
                     /translation="NVEKEEQASQFAAANNAALVRMHSPTLGPAEAPLVVVEFLDPAC
                     ETCAAFYPLVKEILAANPGRIRLVLRYAPFHQGSDQVVAALEAARKQGKFWPALEALL
                     KAQADWAPHHRAEVDRIWKHLEGAGLNLQQLRSDMMAPEIIRVIEQDLIDAQTLKVSK
                     TPDFFVNGQPLPRFGYEPLQDLVAQALAASKR"
BASE COUNT          404 a          598 c          625 g          405 t
ORIGIN      
        1 cgataatgtt caagttgttt cttaacgagt cctaacgcgt gcatctttcg tcgaaaaagc
       61 agcgtatagc gcgctacgct gaaggtggtt ggctctagcc gattccgcgg tggctgtaac
      121 cttcatggca ataaaggtac ctattaacga caagacgatt ccaattgccg caatttttcc
      181 atgtcgcgtg agcctgccct cagaatccct gggggggttg ccgaggccgt aaaggccaaa
      241 accagcgctt agaagcgggc ctagtattgg aacacataaa taacgataca tgtattatgc
      301 tgtcgcatat ccatcccgag acgaggagtt ggccatgcga cagaccgaat tgcatcttac
      361 cgatgaggat cgcgagttga tcgagtcata ccgggcaaag ggtttgcacc acgcgcggga
      421 agtcaatcga gcgcatgtgc tttccgcgct ggatcggggt atcccggagg cgcagatcat
      481 ggcagttctc ggcgtcgggc ggacggtcat ctggcgaaca cgagttgcat acctggaggg
      541 cggcgtggaa tatgcggtgc aggatgtcga acgcccgggt aagccacgga aatacgacac
      601 cgatgtcgag gcgcaagtcg ctgccttggc ttgctcagaa ccgcctgcgg gtgcccagcg
      661 ctggacgctt gccttgctgg aacaagccgc acaagcacag gccggagtgg ggcccatcag
      721 tcgcgaaacc gttcgccggc tgctaaaaaa aactgcatca agccgtggcg caagctgatg
      781 tggtgcatcg gacgactgac agaggagtat cgacaacgga tgtacgatct gctcgacctc
      841 tacgcgcgtc cattccgggc gaaggaacct gttatttgct tggacgagaa gagcaagcaa
      901 ctgctcaagg actcccgcgc gcctttgccc atccgtcccg gaatgcctgt gcggcaggat
      961 tacgagtatg tgcgtgccgg tacctgcaat ctgtttgtgg cggtcgaacc caagggcgga
     1021 cggcgaacgg tcgtggtcac cgaccaccgc gccaagaccg acttcgtggc tttcgtccag
     1081 tatctgctcg aacaggtcta tggcacggcg cgccgcgtcc atctggtcat ggacaacctc
     1141 aatacccact tccgcaagtg cttcgaggac gtgcttggag tcaaggaggc gaaagcgctg
     1201 ctgcggcgcg ttgcctttca ctacacccca aaacacgcca gctggctcaa catggctgaa
     1261 atcgaaatcg gtatccttga tcgccagtgt cttgaccggc gtttgccaga ccgcactacg
     1321 ctggtcgccg aggtcgacgc ttggcagcag cgccgcaatg ccgaacagcg cggcatcgcg
     1381 tggtcgttca ctcggcagga tgcggatcag aaaatggcgc ggcattatgt ttcgtaatta
     1441 atggttcgcg atactagcgc ttgctggcgg ccagcgcctg ggcaaccagg tcttgcagcg
     1501 gctcgtaacc gaaacgcggc agcggctggc cattgacgaa gaagtccggc gtcttgctga
     1561 ccttgagggt ttgcgcatcg atcaggtcct gctcgatgac gcggatgatt tccggcgcca
     1621 tcatgtcgct gcgcagttgc tgcagattca ggccggcgcc ttcgaggtgc ttccatattc
     1681 ggtccacttc cgcccggtgg tgcggtgccc agtcggcctg cgccttgagc agggcctcca
     1741 gcgccggcca gaacttgccc tgcttgcgcg ccgcctccag cgccgccacg acctggtccg
     1801 agccctggtg gaatggtgca tagcgcagca ccaggcgaat ccgcccgggg ttcgccgcca
     1861 ggatttcctt gaccagcgga tagaaggccg cgcaggtttc gcaggcgggg tcgaggaatt
     1921 cgaccaccac caacggcgcc tcggccgggc ccagggtcgg cgagtgcatg cgcaccagcg
     1981 ccgcgttgtt cgccgcggcg aactggctcg cctgttcttc cttctcgacg tt
//
LOCUS       DASRUM010000161         3770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_411279, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000161 DASRUM010000000
VERSION     DASRUM010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411279"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /gene="prpB"
                     /locus_tag="VFF22_10595"
     CDS             complement(<1..581)
                     /gene="prpB"
                     /locus_tag="VFF22_10595"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HZX17299.1"
                     /translation="MSLKSPGQRFRDAVAEEHPLQVIGAINANHALLAKRAGFKAIYL
                     SGGGVAAGSLGLPDLGITGLDDVLTDVRRITDVCDLPLLVDVDTGFGASAFNVARTVK
                     SMIKFGAAAIHIEDQVGAKRCGHRPNKEIVTQQEMVDRIKAAVDARTDDSFVIMARTD
                     ALAVEGLNSALDRAAACVEAGADMIFPEAITELA"
     gene            complement(578..1333)
                     /locus_tag="VFF22_10600"
     CDS             complement(578..1333)
                     /locus_tag="VFF22_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011060180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZX17300.1"
                     /translation="MLDVIEPLESSQEDSETLSEQVFRRIQAAIVKGEIAPGSKISEP
                     ELARTYGISRGPLREAIHRLEGQRLLVRVPHIGARVVSLSHAELIELYEIRESLEGMA
                     CRLAAQRMTQAEIDELRAVLDLHEQDAAFKAGVGYYQQEGDFDFHYRIIQGSGNKTLT
                     QMLCGELYQLVRMYRLQFSATPNRPHQAFAEHHRILDAIAERDGELAELLMRRHIGAS
                     KRNIERHYSMSSANAMSMGQGAHPKTAKTRGES"
     gene            1636..2172
                     /locus_tag="VFF22_10605"
     CDS             1636..2172
                     /locus_tag="VFF22_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease"
                     /protein_id="HZX17301.1"
                     /translation="MTLKPSILLLCLLLAPGLSFAADKTVYGLNEYVRLFDLDLKLAA
                     KLDTGAKTASLSARDIKRFKRDGETWVRFYLAIDEAHSSPIERPLARISKIKRRAGDY
                     DPQEEKTYTARPVIEMDVCMGNALRTIEVNLTDRSAFQYPLLIGSEALKRFSALVDPS
                     LKYAAGKPACAPDANLGE"
     gene            2178..3704
                     /locus_tag="VFF22_10610"
     CDS             2178..3704
                     /locus_tag="VFF22_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inactive transglutaminase family protein"
                     /protein_id="HZX17302.1"
                     /translation="MRSLTLHLKVLVILLVSLGILITAYQIFILGIPMTEDETDDLWN
                     IDAKVEFQASPREPVKLQMFVPPLAQDYVSLNESFISNNYGVSINRADGNRKVTWSAR
                     RANGKQTLYYRLVLTKRYSGEQAKAKGPIFRDSIPVEGAEKIAAEALLAPIRQHSADV
                     ETFISETIKRVNNSNDDNVKLLLAGDASTPNKAKAIELLLSIAHVPMERVHTIRLAAD
                     IQQSPELWLRSFNGENWLYFNPESGEQGLPADRLVWWIGDDNLVTLEGGKQAQVSFSL
                     DNSEMNAIRLAKLTDENTDADFLEYSLYGLPLQTQQTFMIMVMIPIGVLVILILRNLG
                     GLQTLGTFTPVLIALAFREMQLGYGIMFFTVITALGLSLRSYLEHLKLQMLPRLSVVL
                     TFVVVLIAAISLFSHKLGLERGLSVALFPMVILTMTIERLSITWEERGGGHAFKVAIG
                     TLFAATLAHLLMSVPELNYFIFTFPAVLMILVGFMLAMGRYRGYRLTELFRFKAFLKD
                     "
BASE COUNT          829 a         1146 c         1009 g          786 t
ORIGIN      
        1 gccaattcgg tgatggcttc cgggaagatc atgtcggcac cagcctcaac acaggctgca
       61 gcacggtcca atgccgaatt caggccttca accgccagcg catcggtacg tgccatgatc
      121 acaaagctgt catcggtacg cgcatcaacg gccgctttga tacggtcgac catttcctgc
      181 tgggtcacga tctctttatt aggacggtga ccgcagcgct tggcgccaac ctgatcctca
      241 atatgaatcg ccgccgcgcc gaacttgatc attgatttga ccgtgcgcgc gacgttaaac
      301 gccgaagccc caaagccagt gtcgacatcc accagcagcg gcagatcgca cacgtcagtg
      361 atgcggcgca catcggtcag tacatcgtcc aagccggtaa tgcccaggtc cggcaagccg
      421 agcgaacccg ccgctacgcc gccgccagac agatagatcg ccttgaaacc ggcacgcttg
      481 gccagcagcg catggttggc gttgatcgcg ccaataactt gcagcggatg ctcttcggca
      541 acggcatcac ggaaacgctg gcctggactc ttcaagctca tgactcacct cgggttttgg
      601 ctgtcttggg atgcgcgccc tgccccatgc tcatggcgtt agcggagctc atggagtaat
      661 ggcgctcgat attgcgtttg gaggcaccga tgtgtcggcg catcagcagc tcggccagct
      721 cgccgtcacg ctcggcaatg gcatcgagaa tgcggtggtg ttcggcaaac gcctggtgcg
      781 gccgattggg tgtggcggag aactgcaggc gatacatgcg caccagctgg tagagctcgc
      841 cgcagagcat ctgggtcagg gtcttgttgc cgctgccctg gataatccgg tagtgaaagt
      901 cgaaatcgcc ttcctgctgg taatagccga caccagcctt gaaggcggcg tcctgctcat
      961 gcaggtcgag taccgcacgc agctcatcga tttccgcctg ggtcatgcgc tgggcagcca
     1021 ggcgacaggc catgccttcc agcgactcgc ggatttcata gagttcgatc agctcggcat
     1081 gactaagcga gactacccgc gcgccaatat gcggcacgcg aaccagtaga cgctggcctt
     1141 ccaggcgatg gatcgcttcg cgcagtgggc cacggctgat gccgtatgtg cgcgccagct
     1201 caggctcgga aatcttgctg ccgggagcaa tctcgccttt gacgatagct gcctgaatac
     1261 gccggaaaac ctgctcggaa agggtttccg agtcttcctg actggactca aggggctcaa
     1321 ttacatcaag catattgtcg acactgtgcg aattaattgc ctaaaaatta cgcctagaca
     1381 gcattactgt caaacaaacc aataatattg ttgacagtat gcagcgctac tttagtcgcc
     1441 ctcagccgcg accttacttg cataaacgcc tataagcgcc tgtcctgact gaaaaagccc
     1501 atgatagaat gctgcccgct taaggcttgc agaggaattt ctgactgcct gctagactcc
     1561 aaacccaggt gcgccaactg caacatgcgc aaccggcatt tgcttgaccc atcatcaaca
     1621 ccatacccag gacttatgac actaaagccc tccatccttt tgctatgcct gctacttgct
     1681 cccggcctga gtttcgccgc ggataagaca gtctatgggc ttaatgaata cgtccgacta
     1741 ttcgacctcg atctgaagct tgccgccaag ctcgacaccg gcgccaagac cgcttcgctc
     1801 agtgctcgcg acatcaaacg cttcaagcgt gatggcgaga cttgggtgcg tttctacctg
     1861 gccatcgacg aggcgcacag cagcccgatc gaacgcccac tggcgcgcat cagcaagatc
     1921 aaacgccgcg cgggcgacta tgatccacag gaagaaaaaa cctataccgc acgcccagtg
     1981 atcgaaatgg atgtctgcat gggcaatgcc ctacgcacaa ttgaagtgaa cttgaccgac
     2041 cgcagtgctt tccaataccc gttgttgata ggctccgaag cacttaaacg gttttcggcc
     2101 ttggtcgacc cgagtcttaa atacgcagca ggcaaacctg cttgtgcccc tgacgcaaac
     2161 cttggcgagt aactcccatg cgctctctta ccctccatct gaaagtcctg gtcatactcc
     2221 tagtgagcct gggcattctg attacggcct atcagatttt tatcctcggc atcccgatga
     2281 ccgaagacga gactgatgat ctatggaaca tcgacgccaa agtcgagttc caggccagcc
     2341 cacgcgaacc ggtcaagctg cagatgttcg tgccgccgct ggcccaggat tacgtcagcc
     2401 tgaatgagag ctttatctcg aataattacg gcgtgagcat caatcgcgcc gacggcaacc
     2461 gcaaagtcac ctggtccgca cgtcgcgcca atggcaagca gaccctctac taccgcctgg
     2521 tgctgaccaa gcgctacagc ggtgagcaag ccaaggccaa gggcccgatc ttccgcgaca
     2581 gtatcccggt tgagggcgcc gagaagattg ccgccgaagc gctgctcgca ccgattcgcc
     2641 aacactcggc ggacgttgaa acctttatca gtgagaccat caagcgcgtt aacaacagca
     2701 atgacgacaa cgtcaaactg ctgctggctg gcgatgcctc aacgccaaac aaagccaaag
     2761 ccatcgaatt gctgttgtcc atcgcccacg taccgatgga gcgcgtgcac accattcgcc
     2821 tggctgccga tatccagcaa agtcctgagt tgtggctgcg tagcttcaac ggcgagaact
     2881 ggctgtactt caacccggaa agcggcgaac aaggcctgcc ggctgatcgc ctggtctggt
     2941 ggatcggtga tgacaacctg gtcacgctgg aaggcggcaa gcaggcgcaa gtcagcttca
     3001 gcctcgacaa tagcgagatg aatgccattc gcctggccaa gctgaccgac gaaaacaccg
     3061 acgccgactt cctcgaatac agcctgtatg gcctgccact gcagacccag caaaccttta
     3121 tgatcatggt gatgatcccc atcggcgtgc tggtgattct gatcctgcgc aaccttggcg
     3181 gcctgcagac actcggcacc ttcaccccgg tactgatcgc cctggccttc cgcgagatgc
     3241 aactgggcta cggcattatg ttcttcaccg tcatcactgc cctgggtctg tcgctgcgct
     3301 cctacctgga gcacctgaag ctgcaaatgc tgccacgcct gtcggtggta ctgaccttcg
     3361 tcgtggtatt gatcgctgcc atcagtttgt tcagtcacaa gctggggctt gaacgcggtc
     3421 tgtcggtggc attgttcccg atggtgattc tgaccatgac tatcgaacgt ctgtcgatca
     3481 cctgggaaga gcgcggcggt ggccatgcct ttaaagtggc tatcggcacc ctgttcgctg
     3541 caaccctggc gcacctgctg atgagcgtgc cggagctgaa ctacttcatc ttcaccttcc
     3601 cggccgtact gatgattctg gtgggcttta tgctggcgat gggtcgctac cgtggctacc
     3661 gcctgaccga actgttccgc ttcaaagcct tcctcaagga ctaatgccat gttcggtctg
     3721 atcaagacgt ggaaagccct agaagccaaa ggcatcatgg gcatcaatcg
//
LOCUS       DASRUM010000162         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_594713, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000162 DASRUM010000000
VERSION     DASRUM010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594713"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..433
                     /gene="uraH"
                     /locus_tag="VFF22_10615"
     CDS             80..433
                     /gene="uraH"
                     /locus_tag="VFF22_10615"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003204518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HZX17303.1"
                     /translation="MGRLTTHVLDAAHGCPGSELKIELYRVEGAQMELLNSVVTNHDG
                     RCDAPVLQGDDYRSGVYQLHFHAGDYYRARGVNLPEQAFLDVVVLRFGIDAGQEHYHV
                     PLLISPYSYSTYRGS"
     gene            complement(632..1258)
                     /locus_tag="VFF22_10620"
     CDS             complement(632..1258)
                     /locus_tag="VFF22_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017339771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HZX17304.1"
                     /translation="MSLETWLLFASAALVVILIPGPLSLLMVSNSLNYGLRRSAPAFI
                     GGVSASICLLSASALGLGALLLASEQLFSLLKIVGALYLFYLAWQSWQESRKAAKPVT
                     AEEQPVNPSFRAMFWKAFGLGASNPKDILFFAAFLPQFINAERPMLGQLLVMIATWAV
                     LDLACKLFYGLSAQGAARYLRSGKGQVWFNRISAALFGGAGAASLLSR"
     gene            complement(1458..2771)
                     /locus_tag="VFF22_10625"
     CDS             complement(1458..2771)
                     /locus_tag="VFF22_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017679214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HZX17305.1"
                     /translation="MQDLIKPGLLDRFFKLTEHRTTIKTELLAGLTTFVTMAYIIFVN
                     PNIMASAGVDHGAAFVATCIGAAIGCFLMGLYANWPVGLAPGMGLNAFFTYTVVGEMG
                     YSWQIALGAVFISGVLFMIMSLSRIREWLLNSIPMSLRFAMGAGVGLFLGLIGLKTAG
                     IVVDSPATLLTMGSFGEPNAILAAVCFLLIAVLSHRNVFGAILFSMLGVTAIGWAMGL
                     VEYNGLVSMPPSLAPTLMALDIAGAFNIAMISVILAFLFVNMFDTAGTLMGVAHRANL
                     VDEDGKIQNLSKALKADSTSSVVGAFVGCPPVTSYVESAAGVAAGGRTGLTAVAVGVL
                     FLCAMFFAPLAGMIPAYATAGALIYVAMLMMSGMAHIDWKDHTDTIPAIVTVVMMPLT
                     FSIANGIALGFLTYATLKLLTGQRDKVSLSLYVLCIIFIAKFAFL"
BASE COUNT          647 a          817 c          881 g          559 t
ORIGIN      
        1 tgataaatct gccgaattca ataaaagtag aggaggtgcg gcgtctacgt aggcttatct
       61 gcgcaggtgc ccataagcga tgggacgatt gaccacacac gtactggatg ccgcccatgg
      121 ttgcccaggc agcgaactga agattgagtt gtatcgcgtt gaaggggctc agatggagct
      181 gctcaatagc gtagtcacca accacgacgg ccgttgcgat gcgccggtgc tgcagggtga
      241 cgattatcgt agcggggttt accagctgca cttccatgcc ggcgattact accgcgctcg
      301 cggcgtcaat ttgcctgagc aggcctttct cgatgttgtg gtgctgcgct tcggcatcga
      361 tgccgggcag gagcattacc acgtgccgtt gctgatttcc ccttacagct actccaccta
      421 ccgcggtagc tgaaccgaat gcttgtcacc ctctgactca atttaagagt ccttagcccg
      481 tacacactgc gggctttttt tgcccgcgat ttgtcgggtt tcaagcgctg gagtaggagc
      541 gggcgcgacg cgcagtccca tgcccgcgaa gctttgcggc atatgcctat cgcgggcatg
      601 gcccgctcct acatggtgtt gactgcgctg cttagcggct aagcagcgaa gctgcaccgg
      661 ccccgccaaa caaggcagcg ctgatgcggt tgaaccacac ctggcccttg cccgagcgca
      721 ggtaacgcgc agcgccctgg gcgctgaggc cgtagaacag tttgcaggcc aggtccagca
      781 ctgcccaggt ggcgatcatc accagcagct ggccaagcat gggccgctcg gcgttgatga
      841 actgcggaag gaaggcggcg aagaacagaa tgtccttggg gttactggcg cccaggccga
      901 aggccttcca gaacatggcg cgaaagctcg gattgaccgg ctgctcttcg gccgtcaccg
      961 gcttggcggc tttgcgcgat tcctgccagc tttgccaggc gaggtaaaac aggtaaagcg
     1021 cgccgacaat cttcagcagg ctgaacaact gctccgaggc cagtagcagc gcgccaaggc
     1081 cgagtgccga ggcgctcaac aggcagatcg aggctgacac cccaccaata aaggccgggg
     1141 ccgagcggcg caggccatag ttcaggctgt tgctgaccat caacagtgac agcggcccgg
     1201 ggatcagaat caccaccagt gcagcacttg cgaacaacag ccaggtttcc aggctcatta
     1261 gcgattctcc agatgcgcaa aagccctgac gccatgtcag ggcttcggtc ttgcttggtt
     1321 catgttcctt tgttttgaaa atgtagcgag cgatgactag gcaaggcgaa gtcaggcgaa
     1381 aaagcggagt gtacgactgt acatgagcat tttgagcctg acttcaacgc tgcatagtcg
     1441 agcgcagtag ttttcaatta caggaaggcg aacttggcga taaagataat gcacagaacg
     1501 tacaagctca gcgatacctt gtcgcgctga ccggtcagca gtttcagcgt cgcgtaggtc
     1561 aggaagccca gggcaatacc gttggcgatg gagaaggtca gcggcatcat gaccacggtg
     1621 acgatcgccg ggatggtgtc ggtgtgatct ttccaatcga tatgcgccat gccgctcatc
     1681 atcagcatcg ctacataaat cagcgcgcca gcggtggcgt aggcggggat catcccagcc
     1741 agcggggcaa agaacatcgc acagagaaac agcacgccca cagcaacagc cgtcagacca
     1801 gtacggccac cggcagcaac gcctgcagca ctttccacat aactggttac aggcgggcaa
     1861 ccgacaaagg caccaaccac gctggaggta ctgtcagcct tgagagcctt ggacaggttc
     1921 tggatcttgc cgtcttcatc caccaggttg gcgcgatggg caacgcccat cagggtgccg
     1981 gcggtgtcga acatgttgac gaagaggaag gccagaatca cgctgatcat ggcaatgttg
     2041 aaggcgccgg cgatatccag tgccatcaag gtcggtgcca agcttggcgg catggaaacc
     2101 aggccgttgt attcaaccag gcccatggcc cagccgatgg cggtgacgcc aagcatgctg
     2161 aataggatgg cgccaaacac gttgcgatgg ctaagcacgg caatcagcag gaagcacaca
     2221 gcagccagga ttgcattcgg ttcaccgaac gagcccatgg tcagcagagt ggccgggctg
     2281 tcgacgacga tgccagcagt tttcaggccg atcagcccga ggaacaaacc cacccccgca
     2341 cccatggcaa agcgcaggct catggggatg ctgttaagca gccactcgcg gatgcgtgac
     2401 aggctcatta tcatgaacag cacgccggag ataaacacag cgccgagggc gatctgccag
     2461 ctgtagccca tttcaccgac aacggtgtag gtgaagaagg cattcagacc catgccgggt
     2521 gccaggccga ctggccagtt ggcgtaaaga cccatgagga agcagccgat ggccgcgccg
     2581 atgcaagtgg caacaaacgc tgcgccatgg tcgacgccgg ccgaagccat gatgttggga
     2641 ttgacgaaga tgatgtaagc catagtgacg aaagtcgtca ggcccgccag cagctcggtt
     2701 ttgattgtgg tgcggtgttc ggtcagcttg aagaagcggt caagcagacc gggcttgatc
     2761 aggtcttgca tctgcgtcgc ctgttgttct tgtttagcgc tttccacagg ggtactcctc
     2821 atctcttgtt gtgtgccacc cagagcctgt gctgcgcact gctcgactct tgaggtaggt
     2881 ggtgtttttt gccttgctgg cgtg
//
LOCUS       DASRUM010000163         8464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2094178, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000163 DASRUM010000000
VERSION     DASRUM010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2094178"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1276
                     /gene="dprA"
                     /locus_tag="VFF22_10630"
     CDS             161..1276
                     /gene="dprA"
                     /locus_tag="VFF22_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZX17306.1"
                     /translation="MSQLSAISLAVSPAELEARLRLHCLPELGPRRFRKLLSAFDSAS
                     AALSAPASAWRSLGLPQACNDARRSAEVRERAAQSLAWLDASSHHLLMWDDAIYPGLL
                     AELNDAPPLLFVAGDPALLERPQLAMVGSRRASRPGLDTAHSFARSLAGGGFVITSGL
                     ALGIDGAAHQGALDAGGKTVAVLGTGLQCLYPARHKKLAADIVARGGALVSELPLDSP
                     PQASNFPRRNRIISGLSVGVLVVEASPSSGSLITARLAAEQGREVYAIPGSIHHPGAR
                     GCHQLIREGATLVESIEDILQALRGWQAQPPASTVRTPELLNHPLLTLLHAAPHSSEA
                     LVIASGLPLAEVLAALTELELDGRVACENGRWVGRAL"
     gene            1318..1875
                     /locus_tag="VFF22_10635"
     CDS             1318..1875
                     /locus_tag="VFF22_10635"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZX17307.1"
                     /translation="MVSNWQIQQAAQVVRNGGVIAYPTEAVWGLGCDPWHDEAVERLL
                     ALKERPVHKGLILVAADIEQFDFLLDDLPEIWLARLAGSWPGPNTWLVPHQNRLPEWI
                     SGQHDSVALRVSDHPQVRALCRLTGPLVSTSANPAGRPSARSRLRVEQYFPGQLDCVL
                     GGALGGRKNPSLIRDLISGDVVRAS"
     gene            complement(1891..2868)
                     /locus_tag="VFF22_10640"
     CDS             complement(1891..2868)
                     /locus_tag="VFF22_10640"
                     /EC_number="1.6.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HZX17308.1"
                     /translation="MAMRIQFSRHGGPEVLEYTDYSPAAPGPNEVRVQNQAIGLNFID
                     TYFRSGLYAPPALPSSLGTEGAGIVEAVGSAVSGFQVGDRVAYATGPLGAYSELHVLP
                     AEKLVKLPDAISFEQAAAVMLKGLTVQYLLRQTYPLQGGETILFHAAAGGVGSFACQW
                     AKALGVKLIGTVSSAEKAARAKEQGAWAAIDYSHENVAQRVLELTDGKKCPVVYDGVG
                     KDTWETSLDCVAPRGLLVSFGNASGAVTGVNLGILAQKGSLFVTRPTLAGYADTPSRL
                     QSMADELFAMIASGQLQVEISNRYALKDAALAQTALSARQTTGSSILLP"
     gene            3052..3969
                     /gene="hemF"
                     /locus_tag="VFF22_10645"
     CDS             3052..3969
                     /gene="hemF"
                     /locus_tag="VFF22_10645"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HZX17309.1"
                     /translation="MSDRTEAVKAYLLDLQDRICTALQAEDGGAEFFEDAWQRPAGGG
                     GRTRVMENGALIEKGGVNFSHVFGDSLPPSASAHRPELAGRGFEALGVSLVIHPENPY
                     VPTSHANVRFFSAEKAGEEPVWWFGGGFDLTPYYGNEQDCRHWHQVAHDACAPFGAEV
                     YPKFKEWCDRYFHLKHRGEPRGIGGLFFDDLNQWDFDTSFAFMRAIGDAYIAAYLPIV
                     QRRKLTPYGEREREFQAFRRGRYVEFNLVFDRGTLFGLQSGGRTESILMSLPPHVRWG
                     YDWKPEPGSEEARLTEYFLTDRDWLSAKA"
     gene            4059..4883
                     /gene="aroE"
                     /locus_tag="VFF22_10650"
     CDS             4059..4883
                     /gene="aroE"
                     /locus_tag="VFF22_10650"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZX17310.1"
                     /translation="MDRYGVFGNPIGHSKSPLIHRLFAQQTGEQLSYEALLAPLNDFS
                     GFARAFFAEGLGANVTVPFKEQAFALADQLSARARRAGAVNTLKKLADGSLLGDNTDG
                     AGLVRDLTVNAGVSLKGKRILLLGAGGAVRGVLEPLLAEQPAALVIANRTVEKAEQLA
                     REFADLGPVVASGYDWIDAPVDLIINGTSASLAGELPPIAPSLIQPGHTQCYDMMYGK
                     QLTAFNRWAAEHGAARTLDGLGMLVEQAAEAFALWRDVRPASAPVLAELRSLLAEG"
     gene            4888..5514
                     /locus_tag="VFF22_10655"
     CDS             4888..5514
                     /locus_tag="VFF22_10655"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX17311.1"
                     /translation="MGLSADELAQISALTLRHYQDCAEDFRAGTRDHDVSQNIAALLT
                     HIQATAPYQILDFGCGPGRDLRTFSAMGHIAVGLDGCARFVEMARADSGCEAWQQDFL
                     ALDLPPERFDGVFANASLFHIPRQELPRVLQQMHATLKPGGVLFSSNPRGDNQEGWNG
                     ERYGAYHDLANWRVLLNAAGFSELQHYYRPAGLPRDQQPWLASVWRRS"
     gene            complement(5705..>8464)
                     /locus_tag="VFF22_10660"
     CDS             complement(5705..>8464)
                     /locus_tag="VFF22_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003174268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HZX17312.1"
                     /translation="RYADILLPGALWAEAEGVMINSERNLTLMPQAVEPPGEAMADWQ
                     IIAKVACAMGFADGFSYESAEQVFEEIKRFHNRKTGYDLRGASYPRLRQTPLQWPCAS
                     EEGDGRMPIRYQNDGISQTLHIRDDGSTPRLAFATPSGKAQFFPRPHLDPAEMPDDDF
                     PFVLNTGRLQHQWHTLTKTGKVTTLNKLNPGPFIEVHPQDAAALGVRDRDQLEVRSRR
                     GHAVLPVQISDRVLPGGCFAPFHWNDVYGDNLAINAVTSDAIDPISQQPEFKFCAVSL
                     RRVELITHQRLDSPAAEAVDSTRLSHPPEELAMSSIETFAALVGVDSSAPPPLSESER
                     TYMAGYISGLRSNPGGGVPSLPSQAPLAEPTRLWLDGMLAGIFSRVAPAALATSPAVT
                     LLWASQTGNAEALAERLAAHLSEAGIAVALSCMADYPPQQLASAGSVLLLSSTFGDGD
                     APDNGQAFWSALKTSEMRLENLRYAVLALGDPNYEQFCQHGKNLDRRLAELGATPLRP
                     CLDCDSDFDDAAAAWLSELLPLLRPTEKTVPAHSAAATEPVSTQLVSKTQPLPARLAL
                     NLRLNAAGASKDTRQFAFALGDSGLAYEAGDALGVWPRNCPELVEEVLELARLDGEQL
                     VNVAKVGELPLREALSSHFEIARPSNEALALVAERSTSNELRSLLGERKAELKDWLWG
                     RQLADVLQAYPAEHTAGELLGSLKRLQPRLYSISSSPKAHAGEVHLTVSAVRYGKRKG
                     VASTFLADRADNAEVPLFVQSNKYFRVPQDGDVPMIMIGPGTGVAPFRGFLHERRARG
                     DAGKNWLFFGEQHAASDFYYRDELTAMQRDGLLTKLSLAFSRDQAQKIYVQDRIREQG
                     GELWRWLEEGAQLYVCGDASRMAKDVEQALRDVAREHGGLSEEKAAEHIRRLAEQKRY
                     LRDVY"
BASE COUNT         1367 a         2656 c         2780 g         1661 t
ORIGIN      
        1 gacaagctca gctatggcct ggtgctggga gcttctcgct cgcttgagct gatggacaaa
       61 gtgcgtaacc cgtaataccg aaagccccgc aaatgcgggg ctttttcccc gtctgcattt
      121 cctgccgccc acgcggcgcg actgatcaag gatgatccgt atgtcgcaac tctctgctat
      181 ttctcttgcc gtctccccag ctgagctgga agcccgtctg cgcctgcatt gcttgccgga
      241 gttgggacct cggcgctttc gtaaactcct cagtgccttc gacagtgcgt ctgccgcgct
      301 tagcgcgccg gccagtgcct ggcgctctct gggtttgccg caggcctgca atgacgcgcg
      361 gcgcagcgcc gaggtgcgtg agcgcgctgc ccagagcctg gcctggttgg acgcctcatc
      421 ccaccacttg ctgatgtggg acgacgcgat ttatccgggt ctgctggccg agcttaatga
      481 tgccccgcca ttgctgttcg tcgccggcga cccggccctg ctggagcgcc cgcaactggc
      541 catggtcggc agtcgacggg cctcgcgtcc tgggttggat acggcgcata gctttgctcg
      601 cagcctggcc ggcggcggct ttgtgattac tagcggtttg gctttaggta tcgatggcgc
      661 ggctcatcag ggcgcactgg atgccggtgg caagacggtg gcggtactcg gcactggctt
      721 gcagtgtttg tatccggcgc ggcacaagaa actcgccgcc gatatcgtcg cccgtggtgg
      781 cgcactggtc tccgagttgc cgctggattc gccgccccag gcgagcaatt tcccacggcg
      841 caatcgcatc atcagtggtc tttccgtggg cgtgctggtg gtggaggcca gtccgtcgag
      901 cggctcgctg atcaccgcgc gcctggcggc tgagcaggga cgtgaggtct atgcgattcc
      961 cggttctatc catcaccccg gcgcccgtgg ctgtcatcag ttgattcgcg aaggtgcgac
     1021 cttggtggaa agtatcgagg atattctgca ggccttgcgc ggctggcagg cgcagccacc
     1081 cgctagcaca gtgcgaacac cagaactgct taaccatcca ttactgacct tgctgcatgc
     1141 cgcgccgcat agcagcgaag ccttggtgat agccagtggt ttgccgctag ccgaagtgct
     1201 cgctgcgttg accgagctgg aactggacgg ccgcgttgca tgcgaaaatg gccgctgggt
     1261 gggccgcgcg ctttagcttg gatacactgc ccgtcaagct ttagcggaga gcagtgaatg
     1321 gtcagcaact ggcagataca gcaggccgca caggtggtgc gcaatggtgg agtaattgcc
     1381 tatccgaccg aagcagtatg ggggttgggc tgtgatcctt ggcatgacga agcggtggag
     1441 cgtttgctgg cgttgaagga gcggcccgtg cacaagggac tgattctggt cgccgccgat
     1501 atcgaacagt tcgactttct tctcgatgat ctgccggaaa tctggctagc gcggttggcc
     1561 ggcagctggc cgggtcccaa cacctggctg gtgccgcatc agaaccgtct gcccgaatgg
     1621 attagcggcc agcacgacag tgttgccctg cgtgtcagcg atcatccgca ggtgcgcgcg
     1681 ctgtgtcgcc tgaccgggcc gctggtgtcg acttcggcta acccagctgg ccgcccgtca
     1741 gcgcgttcgc gtctgcgggt cgagcagtat ttccccggtc agctcgactg tgtcttgggc
     1801 ggtgctctgg gcggacgcaa gaaccccagc ctgatccgtg atctgatcag cggtgatgtg
     1861 gtcagggcgt cctgaccaca gtctaaccgg ctagggcagc aggatgctcg agccggtggt
     1921 ctgccgcgcg ctcagggcgg tttgcgccag cgccgcatcc ttgagcgcat agcggttgct
     1981 gatctccacc tgcagctggc cgctggcgat catggcgaac agttcgtccg ccatgctttg
     2041 cagccgggaa ggcgtgtcgg cgtagccggc cagggtcgga cgggtgacaa acagcgagcc
     2101 cttctgcgcc aggatgccca ggttgacgcc cgtgacggcg ccagacgcat taccgaagct
     2161 gaccagcagg ccgcgggggg cgacgcagtc cagcgaggtt tcccaggtgt ctttgccgac
     2221 gccgtcgtag accaccgggc acttcttgcc atcggtcagt tccagcacgc gctgggcaac
     2281 gttttcatgg ctgtagtcga tggccgccca ggcgccctgc tctttggccc gtgcggcttt
     2341 ttccgccgag ctgaccgtgc cgatcagctt gaccccaagc gccttggccc attggcaggc
     2401 gaaagagcca accccaccgg cggcggcatg aaacagaatg gtttcaccgc cctgcagcgg
     2461 ataagtctgg cgcagtaggt attgcaccgt caggcccttg agcatcacag cggcggcttg
     2521 ctcgaagctg attgcatccg gcagtttcac cagcttctcg gccggcagca catgcagttc
     2581 gctgtaggcg cctagcggtc cagtggcata ggccacgcgg tcacccacct gaaagccgct
     2641 cactgcagag cccaccgcct caacaatccc cgcaccctcg gtgcccaggc tggacggcag
     2701 ggcaggcggc gcatacagac cgctgcggaa ataggtatcg ataaaattca gaccaatcgc
     2761 ctggttctgc acccgcactt cattagggcc tggggcggcg gggctgtagt cggtgtattc
     2821 gagaacttcc gggccgccgt ggcggctgaa ctggatacgc atggccattt tcaggttcct
     2881 cagcgattga atgcctggat gatgcccgca ggggcgtaca agtgccgtta tccaatcgtc
     2941 tcccttgacc gccgtcaact gcggccgtgg ggtggcagat gttatgctgg ccgcccattt
     3001 ttccccgcgg ccacgtgccg cctggcctta cccgctttct aaggtgccgc cgtgagtgac
     3061 cgtactgagg ccgtcaaagc ctatctgctg gatctgcaag accgtatctg cactgccctg
     3121 caagccgaag atggcggtgc cgagttcttc gaggatgcct ggcagcggcc agccggcggt
     3181 ggcggccgca cgcgggtgat ggagaacggt gcgctgatcg aaaagggcgg ggtgaacttc
     3241 tcccacgtgt ttggcgacag cctgccgcct tcggccagcg cgcaccgacc cgaactggcc
     3301 ggacgcggct tcgaggccct tggcgtgtcg ctggtgatcc acccggaaaa cccctatgtg
     3361 ccgacctccc acgccaacgt gcgttttttc agcgccgaga aagccggcga agagcctgtc
     3421 tggtggttcg gcggcggctt cgacctgacg ccttactacg gcaatgaaca agactgccgg
     3481 cactggcacc aagtcgccca tgacgcctgc gcgcccttcg gtgctgaggt ctatccgaag
     3541 ttcaaggagt ggtgtgatcg ctacttccat ctcaagcacc gcggcgagcc gcgcggcatt
     3601 ggcgggctgt ttttcgatga cctcaatcag tgggatttcg acaccagctt cgcctttatg
     3661 cgcgccatcg gcgatgccta tatcgcggcc tatctaccta tcgtgcagcg ccgcaagctg
     3721 accccctatg gtgagcgcga gcgtgagttc caggccttcc gccgtggccg ctatgtcgag
     3781 ttcaacctgg tgttcgaccg tggcacgttg ttcggtttgc aatccggcgg gcgtaccgag
     3841 tcgatcctga tgtcgctgcc gccgcatgtg cgctggggtt atgactggaa gccggagccg
     3901 ggcagcgaag aggcgcgcct gaccgagtat ttccttaccg accgtgattg gctctcggcc
     3961 aaggcttaat tgcgtagccc ggatgcaatc cggggaatcc tagcgaccgc cccggatttc
     4021 atccgggcta caaaagctca atcgatacag gatttttcat ggaccgctac ggcgtattcg
     4081 gcaaccccat tggccacagc aaatcaccgc tgatccatcg tctgtttgct cagcagaccg
     4141 gcgagcagtt gagctatgaa gccctgctcg cgccgctgaa cgatttctct ggatttgccc
     4201 gtgcgttctt tgccgagggt ttgggggcga atgtcaccgt gccgttcaag gagcaggcgt
     4261 ttgctctcgc tgatcagctt agcgcccgcg cccggcgcgc tggcgcggta aataccctga
     4321 agaagcttgc cgacggcagc ctgcttggtg ataacaccga cggcgccggc ctggtgcgtg
     4381 atctgacggt gaatgccggt gtaagcctca agggcaaacg tattctcctg ctcggcgcgg
     4441 gtggtgcggt gcgcggtgtg ttggagccgc tactggccga gcaaccggcg gcgctggtga
     4501 ttgccaaccg cacggtggaa aaggctgagc aactggcccg cgaattcgcc gacctgggcc
     4561 cggtggttgc cagtggttat gactggatcg acgcgccggt ggacctgatc atcaacggca
     4621 cctcggccag cctggccggc gagttgccgc cgattgcccc aagcctgatc cagcccggcc
     4681 atacccagtg ctacgacatg atgtacggca agcagctgac cgcctttaac cgctgggccg
     4741 ccgagcacgg cgcggcgcgc accctggatg gcctgggcat gctggtcgag caggccgccg
     4801 aagccttcgc cctgtggcgc gatgtgcgtc cggccagcgc cccggtgctg gccgaactgc
     4861 gcagcctgtt ggccgagggc tgaggccgtg ggattgagcg ctgacgaatt ggcgcagatc
     4921 agcgcgctga ctttgcgtca ttatcaggac tgcgccgaag actttcgcgc cggtacccgc
     4981 gaccacgatg tcagccagaa tatcgccgcc ctgctcacgc atatccaggc cactgcgccc
     5041 taccagattc tcgatttcgg ctgcggcccg gggcgcgatc tgcgcacctt tagcgccatg
     5101 ggccacatcg ccgtcggcct ggacggctgc gcgcgctttg tcgaaatggc tcgtgcagac
     5161 agtggctgcg aagcctggca gcaggacttt ctcgccctcg atctaccgcc tgaacgcttc
     5221 gatggggtgt tcgccaatgc ctcgctgttt catattcccc gccaggaact gccacgggtg
     5281 ctgcagcaaa tgcacgccac ccttaaaccc ggcggcgtgc tgtttagctc caacccccgt
     5341 ggtgacaacc aggaaggctg gaatggcgag cgctacggcg cctatcacga cctggccaac
     5401 tggcgagtgc tgcttaacgc cgcgggtttt agcgagcttc agcattacta ccgcccggcg
     5461 ggcttgccgc gtgaccagca gccctggctg gccagtgtct ggcggcggag ctaacgctca
     5521 gctgtagcct ggattagcgc agcgtaatcc gggaaagggg gctaggtgat gccgtgatac
     5581 ccggattgcg ccctcggctt atccaggcta cgaaactgtc gcagagcctg gctcagcccg
     5641 tagggtggac gtcgcttttc acgtccaccg caatggtgga tcgataaagc gtgatccacc
     5701 ctactcaata cacatcacgc aggtaacgct tctgctcggc cagacggcgg atatgttcgg
     5761 cggctttttc ctcgctcaaa ccaccgtgct cgcgcgctac atcccgtagc gcctgctcga
     5821 catccttggc catccggctg gcgtcgccgc agacatacag ctgcgcaccc tcctccagcc
     5881 agcgccagag ctcgccgccc tgctcgcgaa tgcgatcctg cacgtagatc ttctgcgcct
     5941 gatcacggga gaaggccagg ctgagtttgg tcagcaggcc gtcgcgctgc atcgccgtca
     6001 gctcgtcgcg gtagtagaag tcgctggcgg catgctgctc gccgaagaac agccagttct
     6061 tgccggcatc gccgcgtgcg cggcgctcat gaaggaagcc gcgaaacggc gccacgccgg
     6121 tgccggggcc gatcatgatc atcggcacgt cgccatcttg cggcacgcgg aagtatttgt
     6181 tgctctgcac aaacagaggc acctcggcgt tatccgcacg gtcggcgaga aaggtcgagg
     6241 ccacgccctt gcgcttgccg tagcgcacgg cggacacggt caggtgcact tcgccggcat
     6301 gcgccttggg gctcgaggag atcgaataca ggcgtggttg caggcgcttg aggctgccca
     6361 gcagctcgcc agccgtgtgt tcggccggat aggcttgcag cacatcggcc agttggcggc
     6421 cccacagcca gtccttcagc tcggccttgc gctcgccaag caggctgcgc agctcgttgc
     6481 ttgtgctgcg ttcggcgacc agggcgaggg cctcgttgct cggtcgggca atttcgaaat
     6541 gactgctaag cgcctcgcgc agcggcagct cgccgacctt ggccacgttg accagttgct
     6601 cgccgtccag gcgcgccagt tcgagtactt cctcgaccag ctccgggcag ttgcgcggcc
     6661 atacgccgag ggcatcgccc gcttcgtagg ccagtccgga gtcgcccagg gcgaaggcga
     6721 actggcgggt gtccttgctc gcgcctgctg cgttcaggcg caggttcagc gccagacgcg
     6781 cgggtagcgg ctgggtcttg ctgactaact gtgtgctgac gggctccgtt gcggctgcgc
     6841 tatgtgcagg aactgttttt tcagtcgggc gcagcaacgg cagcagctcg cttagccagg
     6901 ccgccgctgc gtcgtcgaag tcgctgtcgc aatccaggca gggccttagc ggcgtggcgc
     6961 cgagttcggc caggcggcgg tcgaggtttt tgccatgctg gcagaactgc tcgtaattcg
     7021 ggtcgccaag cgccagtacc gcataacgca gattttccag gcgcatctcg ctggttttca
     7081 gcgcgctcca gaaggcttgg ccgttgtccg gtgcatcgcc gtcgccgaag gtgctgctga
     7141 gcagcaacac cgagccggcg ctggccagtt gctgcggcgg gtaatcggcc atgcagctga
     7201 gcgccacggc tatgccagct tcgcttaggt gcgcggccag acgttcggcc aacgcctcgg
     7261 cattgccggt ttgtgaggcc cacaacagag tcaccgccgg gctggttgcc agtgccgctg
     7321 gcgcgacgcg gctgaatatt ccggcgagca tgccgtcgag ccacagacgg gtcggctcgg
     7381 ccaggggcgc ctggcttggc aggctgggta caccgccgcc ggggttgctg cgcaagccgc
     7441 tgatatagcc ggccatatag gtgcgctcgc tttcgctgag gggtggcggc gcgctggaat
     7501 ccacgccgac cagggccgca aaggtttcga tgctcgacat cgcgagttcc tcgggcgggt
     7561 gagataggcg agtgctgtcg actgcctcgg cggcgggcga gtcgaggcgt tgatgggtga
     7621 tcagctcgac acgtcgcagg ctgacggcgc agaacttgaa ttccggctgc tgcgagatgg
     7681 ggtcgatggc gtcgctggtt accgcgttga tcgccaggtt gtcgccgtac acatcgttcc
     7741 agtggaacgg cgcaaaacag ccgccgggca gcacgcggtc gctgatctgc accggcagca
     7801 cggcatggcc acgccgcgag cggacttcca gctgatcgcg gtcgcgcacg ccgagggcgg
     7861 cggcgtcctg cgggtgcacc tcgataaagg gcccggggtt gagcttgttc agtgtggtga
     7921 ccttgccggt cttggtcagg gtgtgccact ggtgctgcag gcgcccggtg ttgagcacaa
     7981 aggggaagtc gtcatcgggc atttccgccg ggtccagatg cgggcgcggg aagaactgcg
     8041 ccttgccgct gggcgtggca aaggccaggc gcggcgtgct gccgtcgtcg cgaatgtgca
     8101 gcgtctggct gatgccgtcg ttttgatagc gaatcggcat gcgcccatcg ccctcctcac
     8161 tggcgcaggg ccattgcagc ggcgtctggc gcaggcgcgg gtagctggcg ccgcgcaggt
     8221 catagccggt tttgcggttg tggaagcgtt tgatctcctc gaacacctgc tcggctgatt
     8281 cgtagctgaa gccgtcggca aagcccatgg cgcaggccac cttggcgatg atctgccagt
     8341 cggccatggc ctcgccgggc ggctcgacgg cttgcggcat cagggtcagg ttgcgttcgg
     8401 agttgatcat caccccttcg gcttcggccc acagcgcgcc gggcaggagg atgtcggcgt
     8461 agcg
//
LOCUS       DASRUM010000164        33473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1598241, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000164 DASRUM010000000
VERSION     DASRUM010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 33473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33473
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598241"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFF22_10665"
     CDS             complement(<1..654)
                     /locus_tag="VFF22_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009047101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HZX17313.1"
                     /translation="MSTTPYVLDKTAAEQLLAQVDVHQAMVTMFRELAAGHAMQPAQQ
                     LVEFPNGAGDFINYLGVLAGAGVYGVKTSPYIVREQGPLITAWTLLMSMQSGQPLLLC
                     DAGSLTTARTAATTAVAVDALAAPQASRLAVIGSGAVAQAHVHYAKQLRPWQSIRLYS
                     PGLAGKTGAQLLKLQQLDPRIQLCASQADAIRDADVVMLCTSSAKPLLDPVELDKPAL
                     "
     gene            complement(754..1701)
                     /locus_tag="VFF22_10670"
     CDS             complement(754..1701)
                     /locus_tag="VFF22_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX17314.1"
                     /translation="MLKIALYVCPQTVCSSLSMAMDAFVLANRLAEQRLFELQRVSLD
                     GNAVDLGFAQIQVDGDLSLAEEADLILLAASGSAIDTTLSDNAALLPWLAQRAPTQQL
                     ASLCSSAFLLGAAGVLDGRRATTHWALASQFRQRFPQVNLQIEQLVCEDGQLLSSGGA
                     HAGLDLCLHLIRQHAGDALAQQAANTLVFDSQRGQQSRFAPLLPAPSKDCQLASVLQW
                     LHRHHSEAIDLNRLAAEANCSSRTLLRRFKASTGLTPNDYLQRVRISAAQRALRSPAS
                     LEQIAEQVGYADRATFAKLFKQLCGESPGAFRKRLRQAH"
     gene            1821..2291
                     /locus_tag="VFF22_10675"
     CDS             1821..2291
                     /locus_tag="VFF22_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZX17315.1"
                     /translation="MTHPNAELITRFYQAFQQLDAETMASCYSEDVQFSDPVFTDLRG
                     AAASDMWRMLCAKAQGFSLIFDGVAADDHSGKARWVATYTFSQTGNQVVNHIEANFRF
                     RDGKIVEHRDHFNLWRWASQALGFKGRLLGWAPFVQNAIRQQAGKGLAAFQRSR"
     gene            2329..2649
                     /locus_tag="VFF22_10680"
     CDS             2329..2649
                     /locus_tag="VFF22_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HZX17316.1"
                     /translation="MPDSSSVPEIPAGKPWFVYLVRAANGALYCGISDDPHKRFAKHQ
                     SGKGARFFHSSPAQALVYIESCTGKGDALRRERAVKAMSKLAKEALVAAAPVIAVADN
                     APSG"
     gene            2716..3651
                     /locus_tag="VFF22_10685"
     CDS             2716..3651
                     /locus_tag="VFF22_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009047115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZX17317.1"
                     /translation="MHELILHHYDASPFAEKARLMLGFKQLSWRSVDIPRIMPKPDLV
                     ALTGGYRKTPVLQVGADIYCDTALIARRLDAEKATPALFPEGQEFVAASFAQWVDSQI
                     FQHAVSLVFQPESVAVRFGKLPPEAVKAFVADRAGLFSGGSATRVPVEVAKHNWPSLM
                     GRVEQQLAREAGDFLFGEPSIADFSLAHCLWFLKGTPVTAPLVDAYPHVSAWLGRVLG
                     FGHGASSALSAEDAIAIARAATPAALPDEPFVEPNGLTVGQRVQIAATDYGVDPVEGE
                     LVFAGAEELILRREDDRAGTVHVHFPRLGFAIKAL"
     gene            complement(3685..4002)
                     /locus_tag="VFF22_10690"
     CDS             complement(3685..4002)
                     /locus_tag="VFF22_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HZX17318.1"
                     /translation="MKKVILLLSALALFQHWDTLQRWFQPPLADRPGEVVLYATQWCG
                     YCAKTREQLAQDQIAYREIDIEKDPAGQATFKALGGRGVPLLDIKGTLIHGYDPQAMR
                     AAY"
     gene            complement(4049..4867)
                     /locus_tag="VFF22_10695"
     CDS             complement(4049..4867)
                     /locus_tag="VFF22_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HZX17319.1"
                     /translation="MSRLPDSLLDACRCNPAAPFAQDPARDFGLDKQTAKLALTELRP
                     WLNQQQRMLWANRRHALLLILQGTDCSGKDGVIRRVISSFDPQGLRIHSFQKPDACEQ
                     RHDFLWRYQRRLPGLGLLGVFNRSYYEGLISDPLDGLCSAAELPARQQAIQSFETQLI
                     SQSIQPLKCFLQLSRDEQKHRLQERLEKPHKRWKLHSSDLQSYREFDLRQQRWAEQLS
                     ASHSVAAPWYMIPADHRWLRDWLVASLLARELERLKLDWPDTPPPFSLEDLQRC"
     gene            complement(4870..5877)
                     /gene="yejK"
                     /locus_tag="VFF22_10700"
     CDS             complement(4870..5877)
                     /gene="yejK"
                     /locus_tag="VFF22_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HZX17320.1"
                     /translation="MPIRHCIVHLIDKKPDGTPAVLHARDTELAASQAIENMLADLNE
                     SYNAKQGKAWGFFHEESGAYPFSGWLKHYLDEGQDFAAFSRKAVEQLQKLMEESNLST
                     GGHVLFAHYQQGMTDYLVIALLHHSEGVAVNDALDVTAAKHLDLSQLHLAARINISEW
                     RNNANSKQYISFIKGKNGKKVSEYFRDFIGCQEGVDGPGETRTLLKAFSDFVESEDLP
                     EEQAREKTKTLVSYAANQAKQGEPITLDELSGLIDEERPKAFFDHIRNKDYGMAPEFP
                     ADKRTLSQFQRFTGRAEGLSISFEAHLLGSKIEYDESTDTLVIRNLPTQLTDQLKRRK
                     N"
     gene            6257..7867
                     /locus_tag="VFF22_10705"
     CDS             6257..7867
                     /locus_tag="VFF22_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HZX17321.1"
                     /translation="MHNKIVKSGLVIGSLLAAISGEVLAESNKSSSQLFNAFWSPARL
                     GPLECRFGLLNSTTLGLPRCMQASNTAIHLQVFKDIAQANGGNRAAGLPGYQRSIDYV
                     KSTLEGAGYRVEIQPFPFTAYYPQGPGELQALAPTPAVFEWEVDFTYLSQTEDGDVSA
                     PVVAVDIALGAGNLSSSGCEAEDFVDFPSGSIALLQRGTCNFQIKAENAAAAGASGVI
                     IFNQGDTEDRKGLLNATLGDSYAGGVPVFFATYDVGVGLAQSTETQVRMLADVVRERT
                     RTFNLIAESRHGNANNVVMAGAHLDSVYEGAGINDNASGSAALLELALQLQKVRPKNK
                     LRFAWWGAEESGLVGSTYYVSQLPDEQKSKIKAYLNFDMIASPNFAYFIYDGDGSAFG
                     LQGPPGSAAIEKLFEEYYKLRGLPSEGDEISFRSDYAQFFLDGIAFGGLFTGAEVLKT
                     PEQADLYGGTAGIALDPCYHASCDDLTNIAERALEINGDAMAFVTSWLSTSTKVVDDQ
                     IEAATAEKSVARSAQSYDITHWGKHWIK"
     gene            complement(7959..9284)
                     /locus_tag="VFF22_10710"
     CDS             complement(7959..9284)
                     /locus_tag="VFF22_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZX17322.1"
                     /translation="MRRILHDLKILILTNLWIVPVIAGLVGALFYFAAPPPPMHARMA
                     TGAVGGGYHAFGERLQAELELQGFSLELVESAGSEDNLGKLGSGEVELALVQSGQELT
                     LSDEARGKLNGLGVMYREPLWLFLGDKVKFERLADLMKLRLAVGSQGSGTQAVTAALF
                     AANRIEPAQYPKRWQQLGGSSAADALIAGKLDAAFFIGPAENALIQRLAAEPKVRLVS
                     LRRTEAYLARLPYLSQLQVGEGMLDMAQNTPDRDIVTIGPVSTLVAGEEFHPSLTPLI
                     LEAAKTVLKNGSLLDPAGSYPAKPPLSLNTLAEADYYYDKGLPILQRYLPFRIASLAD
                     RYIILAIPLLVLLFPLFKAVGPIYQWRIRARIYRWYKHLREIDQQLYKGKLPSDLDAE
                     IARLERLEDELARVEVPLSYSSELYQLHMHLRYMIERLHALKQRQLVAS"
     gene            complement(9359..10627)
                     /gene="yjeH"
                     /locus_tag="VFF22_10715"
     CDS             complement(9359..10627)
                     /gene="yjeH"
                     /locus_tag="VFF22_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HZX17323.1"
                     /translation="MSRLNQELGLLQGIGLLSTSLLGTGIFVIPALAATAAGEASLWA
                     WMLLIALVLPVAFTFAQLGRHFPHAGGAPHLIGRAFGARMERLSALLFLAVLPVGLPA
                     ALNIASGFWQALFDLSRGQALAIQLTTLAAMLLLGQRPAKASGMIQGAIAVAIIATIA
                     LIWWVGDLPVSSQPLLPPINGSWHLLPAALGVMFWCFVGIEAFTHMGEEFKNPERDFP
                     LALLFGVLLAGLVYWACSVAVLSLHTYGDVQTDAASMPRMLDLLLGDQARWIAATVGY
                     LACFASMNVYMQGFARLIWSLADEGKLPASLAQRNQHGVPARALLLVIIACALCTGLA
                     SVLDLTVDDLIRYANGNFVVIYLLSMAAGAVLLHGIWRWLAAVSCLLCALVLLMLGMD
                     AGYALGLLVVLGLLDRWREQRSMRLAGVSS"
     gene            10757..11176
                     /locus_tag="VFF22_10720"
     CDS             10757..11176
                     /locus_tag="VFF22_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZX17324.1"
                     /translation="MDKFDQQILALLRADARMPVSQIAREVNLSRSAVSERIRQLEQG
                     GIISGYHAQVASPGDAAIKVYLELFYSGGRCEHYVELMRVFPEVRRCSGISGETDMLV
                     YIEAPSMQRFSEVRGEIENFPGMQKVKTHMVVKDWQF"
     gene            11185..12405
                     /gene="sbcD"
                     /locus_tag="VFF22_10725"
     CDS             11185..12405
                     /gene="sbcD"
                     /locus_tag="VFF22_10725"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HZX17325.1"
                     /translation="MRILHTSDWHLGQHFMGKTRQAEHQAFCAWLIEQVRTHQVDAVL
                     IAGDIFDTGAPPSYAREQYNRFIVELRGTGCELVVLGGNHDSVAMLGESKMLLAQLGT
                     RVIPGVCEQLDEQLLVLHRRDGLPGAILCGIPFIRPRDVLLSQAGQSAQDKQQSLQQA
                     IQQHYQDLYSLAEAKRTELGGNLPIIASGHLTTVGASASDSVREIYVGSLEAFPTSAF
                     PPAAYIALGHIHRPQKVGGLEHIRYCGSPIALSFDEARQQKEVLLVELDSSGLRQITA
                     LPVPRFQPLLSLRGSLKELEMQIKQAAEQGNAEQPVWLEVLVGTDDYLSDLQLRIAAL
                     CEGLPVEVLRIRRERGNASASLQGQAKETLDELSVEDVFAQRLSSESLDDAEQARLLG
                     LYQQVVSELREGEV"
     gene            12402..15836
                     /locus_tag="VFF22_10730"
     CDS             12402..15836
                     /locus_tag="VFF22_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HZX17326.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTAEPFKDNGLFAITGPTGAGKT
                     TLLDAICLALYHRTPRMNTVSASANELMSRHTADCLAEVEFEVKGVGYRAFWSQRRAR
                     DKADGALQAPKVELAAADGQIITDKINEKLRETERLTGLDFERFTKSMLLAQGGFAAF
                     LEANANQRAELLEELTGTDIYGQISQRVFEQCREVKTALDQLRAKAEGVELLNEEQRI
                     ALQAQAAQLAIDEAALSTQQAELQRQRRWCEDLSKTQAQLHASQQAEQAAQQDLQLAQ
                     PQLAQLAASEPALRLQPLHHDWQQARQSLAQTEQGLQQNAAEQRQAEQAIAEHSWQAR
                     ELSALLLADCQAQRQQLAERLQQLQQQLAAQPQHGRLGEQLGSWGEQLKARQLQAEEI
                     AQLQLSQQQLQETIAASQTQLNQQTEQLGAEQLRLQQAREQQDAAQQQRDELLVGRSE
                     AALRERWQQLHIQGGVLTQLGELVTVQQRLAAEQSQLTTALTSLREQHQAKGAEVAQL
                     RLSYKDLQQQVQAQEKLLEQEQRIQALESHRAQLQPGEACPLCGSESHPAISAYQALD
                     VSATKALLNSKRAELDALTEQGRQLAGELEKLAARLEQQQQQFERGRQTQAEQGERWQ
                     ALSAQLETPLADAVALAKQQAQQNIELQQLQQQLEQLQQLNTILEQRQQARLNLQQRC
                     SELEQQQALNHLQLQQRQEQTEQGRLRLQQLQDVAHKRNESLSQDLAEFGYQLPDDAS
                     AWLAERQAEWQRWQHNQQHSLDLQRQLSELDHGLQNAAQLAQLWQQRWSVLDLADQPA
                     LAKPADAQAALQQAQVQLEEAQRRQQQLAGSRQALASRLQDEQARLQQAEQQWTKALS
                     ASPFNAEADYLAALLDEAQRVALQQLRQRLDKALSDAQALASAAAQQLAALQASPATA
                     LSLAQLDEQLLVLNTQLKALSERQGEIRAQLQGDDARRLGQQSLFAAISTKQGEYDLW
                     QQLNSLIGSADGAKYRKFAQGLTLDHLVYLANQQLERLHGRYQLARRSSGELELEVID
                     TWQADAARDTKTLSGGESFLVSLALALALSDLVSHKTSIDSLFLDEGFGTLDGETLEV
                     ALDALDNLNASGKMIGVISHVEALKERIPVQLKVHKGVGMGYSGLDKRFAVNTRDN"
     gene            15911..16702
                     /locus_tag="VFF22_10735"
     CDS             15911..16702
                     /locus_tag="VFF22_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX17327.1"
                     /translation="MAKVLICLLCCYCLVVDVHAAPRSAEALTLCYEDQNSYPWVMTD
                     GGGLNLQLLRLVADALPLQFSFVAVPWKRCLSGMAQGTYDGAFASSFKEERLLLGRYP
                     QDADGRLDERKRLHTSIYALYRRKGSPVSWNGEEFRQLHGRVGSLSGFSIVDFIRAQG
                     AEVDETSRDPLALLQMLRHKRIEAAALQSLRGDFVLQSNPELAARLEKVKLPLEEKAY
                     YLMLSNAYVAANPEQAARIWDEIERQRESLTYQQQVRDFLERSQP"
     gene            16772..18058
                     /locus_tag="VFF22_10740"
     CDS             16772..18058
                     /locus_tag="VFF22_10740"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014597310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HZX17328.1"
                     /translation="MAKPPAAPPSASALGRFLTLAGTATRIGGSVLGQSLRPGRGGID
                     WQPVGDLLTDVLGEMKGPVLKLGQMASQWQGVLPEPVALALASLQNRVPALPFSALRG
                     HLQQVYGADLGQFFQQIDEQPFAAASLGQVHRAIAVDGRALVLKVQYPGIAEICQADL
                     RQLRRLLPLGRLFRAPAEQLEGLYQELATVIEAELDYPAEMRRLQDFRAHFADWPGLR
                     LPQPQEDLCRPGVLALSEEAGLPFAEVSQASAEVRERLALTLVRWLSAQAFELGLLHA
                     DPHPGNFAYTAAGELVVYDFGCMQALSAPLLAAYVQTYKALQARDGEQLEKAFQALGT
                     RQPKSTTPYGLYRQLHGLLGPLLQPGVHWDFAATPLHEQVQRLLPDVLGALGSLQPAP
                     ATLLVNRTLEGHYWNLSRLGVALPVADLLQVQLAGR"
     gene            complement(18055..19197)
                     /locus_tag="VFF22_10745"
     CDS             complement(18055..19197)
                     /locus_tag="VFF22_10745"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX17329.1"
                     /translation="MTSRQLLSGLVLSYLLTPGFASAAEPGLSTDEVRIGMVNAQSGP
                     AAALGLGMLNGAQAYFKRVNAEGGVHGRRINLLSRDDGYEPSETAAHTRSLLKTQQVF
                     ALLGYVGTPTSRAAVPLALRAQVPYLFPFTGAEFLRTPVKSWVFNIRASYIEETEQLV
                     ERVTQDLKLSKIAILMQDDSFGESVKGGLNGALLKRDLKIYAQARIQRNSLDVTEAIK
                     ALQRAQPEAVFFVGTYRQLAAAIKQAKALGFNTRFISVSFIGTEGFIREVGNDGDGVY
                     ITQVVPSPHDSSLALVRAYQIDMRPGDFDHASLEGYIGAAVFTQALRQAGAKPTREAF
                     LDALEHLETDLGGFKVAFSRSQHQGSSAVFLTRIENGQAVPVKTMR"
     gene            complement(19316..20110)
                     /locus_tag="VFF22_10750"
     CDS             complement(19316..20110)
                     /locus_tag="VFF22_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX17330.1"
                     /translation="MKWKNLFVALCLMLGLHAQASEQPELKISVGDWPPYLSSELKHN
                     GVIAHLISDLLADEGYRVSFQFLPWPRAYSAAAAGRFDGTAVWMHKAEREADFLFSAP
                     LLDEKFVFFHLKSLPFDWQRFDDLTGMTLGGGLEYSYGADFDAFLAENKVKIERVSND
                     QQNFDKLLKERVVLYPQEMNVGYAALRSHFSAEDQAKITHHPKPLLVNLSYLMLPKSL
                     EGSPALLERFNKRLQHYRDSGRYQRYFDDLQQGKYQPEPSASPVSE"
     gene            20281..21648
                     /locus_tag="VFF22_10755"
     CDS             20281..21648
                     /locus_tag="VFF22_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17331.1"
                     /translation="MLRIPTLCALFALAPLASALELKPLALYQSGFAAGTEIVSVQAS
                     SMRVIVSNSAEGLVDILQLDPAKGLNRIARHNLVAAGTGEITSAIFHPSEDLFAVCIR
                     NSDGLKNGRVELRAAADGKLLNTVEIGIWPDSLAFSPKGDFIVVANEGEGYVRDGESF
                     RSGEGSISLIDLRGGVAAAKHSLIKLPDLAGVEGTTQAEHKRLLERVIDGEELKVPFG
                     TSPEHIEPEYVTFSPDGARAYVSLQENNAIAVVDVLQGKLDKVFGLGTVRHAADIVDD
                     GKYQPTAELFALREPDALAVSADGRYLISADEGDTDPKIAKTKAGLPSGGGRTVSVFD
                     AISGKLLGDTGSQLDDMAAQAGVYPDVRSPNKGSEPEGVVSFDAFGKRLAVATLERAD
                     ALALIDLDQPEQPKVLQVIGAGEGAGSGKLAPEGLAHVEHNGRHFLVTGLEKSGNVAL
                     FELLK"
     gene            22046..23284
                     /locus_tag="VFF22_10760"
     CDS             22046..23284
                     /locus_tag="VFF22_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZX17332.1"
                     /translation="MTVWVVSLLFVLTYLGMAAGGIRGLRVDRSWIAGCAAVLLLVSG
                     AQSPAEATSHLDAGALLLLLALMLISAQFDFSGVYAWLNQQLSHYAEQPALLLLGVVL
                     LGGVLSALLVNDIVAFALTPLLCHSLLARGLDPRPFLLALALSCNAGSSASLIGNPQN
                     ILIGQAGGLDFWSYTFIAGPPALAALAITYAVIWLQWRKRWGTAKPLVQAETPVERIY
                     GLRSYLKPLLASLVLLGLFSTSMPREISALLIAVLVMISRRVDSRDYVQKVDWNLLLL
                     FAGLFVVTGAAMSLPQVAGMAGNLAELGLLPHGVITLASLSLLAGNLIGNVPYVMLLL
                     GVLPDASEALLVGLAIMSTLAGNLLLIGSVVNLIVAESARRQGVPLSFVDFARSGVPV
                     TLLSMGVAGLWLALGGWLPW"
     gene            complement(23423..24451)
                     /gene="rlmF"
                     /locus_tag="VFF22_10765"
     CDS             complement(23423..24451)
                     /gene="rlmF"
                     /locus_tag="VFF22_10765"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008018579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HZX17333.1"
                     /translation="MSLPPKRPSRKPTAPRPAPSKPAAGKPDTAKGQLHPRNRHQGRY
                     DFPALIKASQELAAFVIINPYGNESIDFANPAAVKVFNRALLKQFYGIDHWDIPADYL
                     CPPIPGRADYLHYLADLLAASNGGEIPRGAKVRALDIGVGANCIYPLLGNREYAWQFL
                     GSDIAASAISSAKAIVQANPGLSEAIELRQQSDSTHIFQGLLQSDERFDLTLCNPPFH
                     ASAEEASSGSKRKWRNLGKLDPKRKLPVLNFGGQAAELWCKGGEAAFVSQLIKESVEV
                     GQQVLWFSSLISKAGNLPGVYSALKKAGALQVQTVEMAQGQKQSRFVAWTFQTAEQQQ
                     AWRKARWS"
     gene            complement(24566..24862)
                     /locus_tag="VFF22_10770"
     CDS             complement(24566..24862)
                     /locus_tag="VFF22_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZX17334.1"
                     /translation="MDVSKTRTSFYRRLYVAWLIDSGTATSVPALMAATGMPRRTAQD
                     TLTALAELDIDCAFQQESGERNNAGHYEIRDWGAINPQWIAANLPQIKAVLAYP"
     gene            complement(24918..26294)
                     /gene="nhaD"
                     /locus_tag="VFF22_10775"
     CDS             complement(24918..26294)
                     /gene="nhaD"
                     /locus_tag="VFF22_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HZX17335.1"
                     /translation="MYALMALIFVLGYLCIALEHPLKIDKAASAILTAVICWTLLVLG
                     ADSILPLIGAATAGASGNDHHVTEELRHHLGEISEILFFLMGAMTIVELIDAHEGFKV
                     ITDRIRTNKRVTLLWVVALITFFLSAVLDNLTTTIVMVSLLRKLVADQKERWFYVGIV
                     VIAANAGGAWSPIGDVTTTMLWIGNQVTTSGIIANLFIPSVVCLLVPLLVLSMTMKGE
                     IQRPLLSESDEERRDPTTPFERNLVFILGIGALVFVPVFKTVTHLPPYMGILFGLGVL
                     WVVTEVIHRGKNDEDKHPLSVVGVLRRIDTTSVLFFLGILLAVSSLATAGHLTQVAVA
                     LRESLGNVYAINIAIGLLSSVVDNVPLVAGAMKMYPLITPEALALAGADTAWLSNFQV
                     NGTFWEMLAYCAGTGGSCLIIGSAAGVAAMGMEKINFIWYLKKISLLAFLGYISGAAT
                     YIAIATLS"
     gene            complement(26508..27971)
                     /gene="nhaC"
                     /locus_tag="VFF22_10780"
     CDS             complement(26508..27971)
                     /gene="nhaC"
                     /locus_tag="VFF22_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HZX17336.1"
                     /translation="MTDARSPSLLDALLPIGVLVLLLGLSVYLYGDSSSSGPNQIALM
                     SAAFVAGLVGLKNGLQWAQIEEGILAGIHMAMKANLILLAVGALIGTWILAGTVPTMI
                     WLGLKLISAEYFYVTSCLICALTALSIGSSWTVAGTLGIGLMGVAAGLGLDPAITAGA
                     IISGAYFGDKLSPLSDTTNLAPAAAGAELFAHIRLMLRTTVPVLGIALVLFFILGQRA
                     DGSHDTARIAEVLQALEAQFSLGWHLLLPVALLLFLAVRQWAAFPAVFVAALLGGVFA
                     VIFQPEVMARLADPAAGSLAPLKTVWTALFAGYESSSGNPALDGLLSKGGMASMLTTV
                     WLIICAMCFGGVLEKLGLLQRLLQSTLHLAKSTSSLIASTIATTIGTNIITADQYIAL
                     VLPGRMYRAEYEKRGLAPVNLSRALEDGGTLTSVLVPWNTCGAYMAATLGVATLSYLP
                     YCFFNLLMPLLAICLAFIFPPQPVMPANAERDDLSQA"
     gene            complement(28098..30929)
                     /locus_tag="VFF22_10785"
     CDS             complement(28098..30929)
                     /locus_tag="VFF22_10785"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HZX17337.1"
                     /translation="MDKTYQPHAIETALYQNWEANNYFAPQGAGEPYTIMIPPPNVTG
                     SLHMGHGFNNSIMDALIRFRRMQGRNTLWQPGTDHAGIATQMVVERQLAADGIGRHDL
                     GREKFLEKVWEWKEQSGGTITRQIRRLGSSVDWSRERFTMDDGLSESVKEAFVRLHKD
                     GLIYRGKRLVNWDTKFHTAISDLEVENHDEKGSLWNLRYPLADGNKTADGKDYLIVAT
                     TRPETVLGDSAVAVHPEDERYKALIGTFVELPLVGRRIPIIADDYCDPEFGTGCVKIT
                     PAHDFNDYEVGKRHNLPLLNIFDKNAAVLAQVQAFNVDGSVNSEIDCSLPAEYAGLDR
                     FEARKQIVAAFEAAGLLESIDAHALKVPKGDRSGTIIEPWLTDQWYVSTKPLAEKAIA
                     VVESGEIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDDAGNVYVGRDEAE
                     VRATHNLGDANLRQDEDVLDTWFSSGLWTFSTLGWPQQTEELKTFHPTDVLVTGFDII
                     FFWVARMIMLSTHLTGQIPFKTVYVHGLVRDGQGQKMSKSKGNVLDPLDIVDGITLDE
                     LLEKRTSGMMQPKLAEKIAKQTRAEFPEGIAAYGTDALRFTNLALASTGRDIKFDMGR
                     VEGYRNFCNKIWNAANFVLENTDGQDTGVNGEPVELSSVDRWIISALQRTEAEVTRQL
                     DAFRFDLAAQALYEFIWDEYCAWYLELVKPVLWDETAPIERQRGTRRTLIRVLEVALR
                     LAHPFMPFITEEIWQRIKAQAGVSGETIMLQAWPVANESRIDAAAEGDIEWVKQLMLG
                     LRQIRGEMKISMAKRIDIILQNASAEDLRRLADNAPLLNKLAKLESVRVLEAGEEAPM
                     SATALVGDMQVLVPMAGLIDKDAELARLDKEITRLSGEVQRVGGKLSNEGFVAKAPAE
                     VLDKERAKLAEAEQALSKMVEQRGKIAAL"
     gene            complement(31042..31434)
                     /locus_tag="VFF22_10790"
     CDS             complement(31042..31434)
                     /locus_tag="VFF22_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HZX17338.1"
                     /translation="MDTPKPPQKPAQLLNDLESIRQLLGDENLEPPLLIDSLDPDSIP
                     LLSEIVTPPPVTAAPAPKPPVAQTLRETVSQSVSRDSEMNRLDSELRAAAQLILQDVI
                     DDFVPQIEAELKRRLDARLSRLLPPRRP"
     gene            complement(31444..31863)
                     /locus_tag="VFF22_10795"
     CDS             complement(31444..31863)
                     /locus_tag="VFF22_10795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HZX17339.1"
                     /translation="MPRIEFYVLSTAVPAERLRAACQLAMKAWRAGLPVFLRGSDAQQ
                     CTEVDELLWRFKAESFVPHSLYQDDAQAPVVIGLDEEPSSAQGVLINLGSTLSPQVER
                     FSRVIEIVNQEPDLLTACRENFRSYRQRGYDPKRVEL"
     gene            complement(31964..33454)
                     /locus_tag="VFF22_10800"
     CDS             complement(31964..33454)
                     /locus_tag="VFF22_10800"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HZX17340.1"
                     /translation="MEFIVKSARPETLKTATLVIPVGEGKKLGETAKAIDTASAGAIS
                     ALLKRGDLAGKVGQTLLVHNLPNLKAERVLLVGTGKDSELSDRQLRKLITSVYSSLKN
                     LGGNDAVLALDELQVKGRETYGKTRLMVESLADAGYCFDRFKSQKAEAGALKKLTLLT
                     EKANLADAERASTHAQAIAAGMAFTKDLGNLPPNLCHPSYLAEEAKNLGKAYKNLKVE
                     ILDEKKLKELGAGAFLAVAQGSDQPPRMIVLNYQGGKKTEKPYALVGKGITFDTGGIS
                     IKPAAGMDEMKYDMCGAASVFGTLKAVLELQLPINLVCLLACAENMPSGGATRPGDIV
                     TTMSGQTVEILNTDAEGRLVLCDTLTYAERFKPQAVIDIATLTGACIVALGSNVSGLM
                     GNNDELVQQILGAGLTADDRAWQLPLYDEYQEQLDSPFADIANIGGPKAGTITAGCFL
                     SRFAKNYHWAHLDIAGTAWISGGKDKGATGRPVPMLTQYLLDRSNA"
BASE COUNT         6114 a         9848 c        10832 g         6679 t
ORIGIN      
        1 cagcgccggt ttatccagct cgacgggatc gagcaacggt ttcgccgatg aggtgcagag
       61 catcaccaca tcggcatcgc gaatggcgtc tgcctggctg gcgcacagct ggatgcgtgg
      121 gtcgagttgc tgcagcttga gtagctgcgc gccggttttg ccggccagac ctggcgagta
      181 gagacggatg ctctgccagg ggcgcagctg tttggcgtaa tgcacatggg cctgggccac
      241 ggcgccgctg ccgattaccg ccaggcggct ggcctggggc gcggccaggg catccacggc
      301 cactgcggta gtggcggcgg tgcgcgcggt ggtcaggctg ccggcatcgc agagcagcaa
      361 tggctggccg ctttgcatcg acatcagcag ggtccacgca gtaatcagcg ggccttgctc
      421 gcggacgata tagggcgagg ttttcacccc gtacacaccc gcccccgcga gtacgcccag
      481 gtagttgata aagtcgccag ccccgttggg gaactccacc agctgctgcg cgggctgcat
      541 cgcgtgaccg gcggccagct cgcggaacat agtgaccatg gcctggtgta catcgacctg
      601 ggccagcagc tgttcggcag cggttttatc cagcacataa ggggtggtcg acatgggcgg
      661 ctccgcaacg aattaaacaa ttaaatccat tttggacttt atggtatttt tcgacaagcg
      721 ctgacgcggc atcttgatac gcggtagcga cgttcagtgg gcctgacgta agcgcttgcg
      781 aaacgcccct gggctttccc cgcagagctg tttgaacagc ttggcaaagg tggcgcggtc
      841 ggcataaccg acctgctcgg cgatctgctc cagcgacgcc ggactgcgca gggcgcgctg
      901 cgcggcgctg atgcgcacgc gttgcaggta gtcattgggc gtcagcccgg tgctggcctt
      961 gaaacggcgc agcaaagtgc gcgatgagca gttggcttca gcggccaggc ggttgaggtc
     1021 aatggcttcg ctgtgatgac ggtgtagcca ttgcaacacg gacgcgagct ggcaatcctt
     1081 gctgggcgct ggcagcagcg gggcaaagcg tgactgctgg ccgcgctggc tgtcgaacac
     1141 caacgtgttg gctgcctgtt gcgccagtgc gtcaccggca tgctggcgga tcaggtgcag
     1201 acacagatca agcccggcgt gcgccccgcc ggagctgagc agctggccat cttcgcagac
     1261 cagttgctcg atctgcagat tgacctgcgg gaaacgctgg cggaactggc tggccagcgc
     1321 ccagtgggtg gtggcgcggc gaccgtcgag cacgccggcg gcgcccagca gaaaggcgct
     1381 gctgcacagg ctggccagtt gttgggtggg cgcgcgttgc gccagccagg gcagcagggc
     1441 ggcgttatcg ctcagggtgg tgtcgatggc gctaccgctg gcggcgagca gaatcaagtc
     1501 ggcctcttca gccaggctca gatcgccgtc gacctgaatc tgcgcaaagc ccagatcaac
     1561 tgcgttgccg tccaggctga cgcgctgtag ttcaaacagc cgttgttcgg ccaggcggtt
     1621 ggccaggaca aacgcatcca tggccatgct caggctggag cacacggttt gcgggcagac
     1681 atacagggcg atcttcagca tgacgtgctc agacgtgatg gcttttggcg attattgaca
     1741 caaatttgtc ttttgcgcca atcgcgcggc ggcgatttaa cgacaatact gctcgcacgt
     1801 tccctaccgg agttcgcccc atgacccacc ccaacgccga gctgatcacc cgtttctacc
     1861 aggccttcca gcagctggat gccgagacca tggccagctg ttacagcgag gatgtgcagt
     1921 tcagcgaccc ggtgtttacc gacctgcgcg gcgcggctgc cagtgatatg tggcgcatgc
     1981 tctgcgccaa ggctcagggc ttttctctga tctttgatgg cgtggcggcg gatgatcact
     2041 ctggcaaggc acgttgggta gcgacctata ccttcagcca gaccggtaat caggtggtca
     2101 accatatcga ggccaacttc cgctttcgcg atggcaagat cgtcgagcac cgtgatcact
     2161 tcaacctgtg gcgctgggcc agccaggcgc tgggctttaa aggtcgcctg ctgggttggg
     2221 cgccgtttgt gcagaacgcc attcgccagc aggctggtaa agggttggct gccttccaga
     2281 ggagccgtta agctcggcaa ccttgcttat tgcctgccga tttaactgat gcctgactcc
     2341 tcttctgtgc ctgaaatacc tgccggcaag ccctggtttg tctatctggt gcgggcggcc
     2401 aatggcgcgt tgtactgcgg cattagcgac gatccgcaca agcgttttgc caagcaccag
     2461 agcggtaaag gcgcgcggtt tttccactcc agtccggcgc aggcgctggt gtatatcgaa
     2521 agctgtaccg gcaaaggcga tgcgctgcgt cgcgaacggg cggtcaaggc catgagcaag
     2581 ctggccaagg aagctctggt ggccgccgcg cctgttatcg ctgtagctga taacgcgcca
     2641 tcaggctaag cgggtaggcc ggcacggctt gcgcgggtaa gctgcgggtt tacctgaccc
     2701 tttgcggagc ctgccatgca cgagttgatc ctgcaccatt acgatgcctc accgtttgcc
     2761 gagaaggcgc gcctgatgct gggtttcaag cagctgtcct ggcgctcggt ggatattccg
     2821 cgaatcatgc ccaagcccga tctagtggcg ctgaccggtg gctaccgcaa gactcctgtg
     2881 ctgcaggtgg gtgccgatat ctactgtgat actgcgctga ttgcccgtcg cctggacgcg
     2941 gagaaagcaa cccctgcgct gttccctgaa ggccaggagt tcgttgcggc gagctttgct
     3001 cagtgggtcg attcgcagat ttttcagcat gcggtgagcc tggtgtttca gccggaatcc
     3061 gttgccgtgc gtttcggcaa gctgccgcct gaggcggtca aggcctttgt cgcagaccgt
     3121 gccggactgt tcagtggcgg tagcgcaacg cgtgtaccgg tcgaggtggc caagcacaac
     3181 tggccgtcgc tgatggggcg ggtagagcag caactggcgc gcgaggcggg cgacttcctg
     3241 tttggtgagc cgtcgattgc tgacttctcc ctggcccatt gtctgtggtt cctcaaaggc
     3301 accccagtga ccgcgccgct ggtcgatgcc tatccgcatg tcagcgcctg gctcggccgc
     3361 gtgctgggct tcggtcacgg tgcgtccagt gcgctgagtg cagaagatgc tattgccatc
     3421 gcccgtgcgg cgacgccggc ggctctgccg gacgagccgt tcgtggaacc caacggcctc
     3481 acggtcggtc agcgggtgca gattgccgcc accgattacg gtgtcgaccc ggtagagggt
     3541 gagctcgtgt tcgctggcgc tgaagaactg atcctgcgcc gcgaggatga tcgtgccggt
     3601 accgtgcatg tgcacttccc gcgactcggt tttgccatca aggcgctgta agcaccaggt
     3661 tacgacctac tgcgcgctgg cttttcagta ggcagcgcgc atcgcctgcg gatcgtaacc
     3721 gtgaatcagc gtgcccttga tatccagcaa gggcacgccg cgcccaccta gggccttgaa
     3781 ggtggcctgg ccggctgggt ctttctcgat atcgatttcc cggtaggcaa tctggtcctg
     3841 cgccagttgc tcacgggttt tcgcgcaata gccgcaccat tgcgtggcat acaacaccac
     3901 ttcgcctggt cgatcagcca gcggcggttg aaaccagcgc tgcagcgtgt cccagtgctg
     3961 gaacagtgcc agggcgctga gcagcaggat gactttcttc atgggcgttc ctttgccttg
     4021 ttgggttgag ggtctggtga atcgtgggct aacagcgctg caagtcttcc agactgaacg
     4081 gcggcggggt atccggccag tccagtttta gccgttccag ctcgcgggcc agcagactgg
     4141 ccactaacca atcgcgcagc cagcgatgat ccgccggaat catataccag ggcgcagcaa
     4201 cgctatggct ggcgcttagt tgttcggccc agcgctgttg gcgcaggtcg aattcgcggt
     4261 aggactgcag gtcgctgctg tgcagcttcc agcgtttatg aggtttttcc aggcgctctt
     4321 gcaggcggtg cttttgttca tctcgggata gctgcaaaaa gcatttcagc ggctgaatcg
     4381 actggctgat cagctgcgtc tcgaagctct gaattgcctg ctggcgcgca gggagttcgg
     4441 cagcactgca caggccatca agcggatcgc tgatcagccc ttcgtagtaa ctgcggttga
     4501 atacccctag caggcccagc cctggcaaac gccgctggta gcgccagaga aagtcatggc
     4561 gttgttcgca ggcatcgggt ttctgaaaac tgtggatacg cagaccctgg ggatcgaagc
     4621 tgctgatcac ccggcgaatc acgccatcct tgccggagca atcggtgcct tgcaggatca
     4681 gcagcagggc atgacgccgg ttggcccaga gcatgcgctg ttgctggttg agccaggggc
     4741 gcagttctgt cagagccagt ttggccgttt gcttatccag gccgaaatcg cgcgccgggt
     4801 cctgggcaaa gggcgctgcc ggattacagc ggcaggcatc cagcaggcta tccggcaggc
     4861 ggctcatgct tagttcttgc ggcgcttgag ttgatcggtc aactgcgtcg gcaggttacg
     4921 gatcaccagg gtgtcagtgc tttcgtcgta ctcgatcttc gagccgagca ggtgagcttc
     4981 aaagctgatt gataggcctt cggcgcgccc ggtaaagcgc tggaactggc tgagggtgcg
     5041 tttgtccgcc gggaattccg gggccatgcc gtaatcctta ttgcggatat ggtcaaagaa
     5101 ggccttaggc cgttcttcgt cgatcagtcc ggagagctcg tcgagggtga tgggctcacc
     5161 ttgtttggcc tggtttgcgg cgtagctgac caaggtcttg gtcttctcgc gggcttgttc
     5221 ttctggcagg tcttcgcttt ccacgaagtc gctgaaggct ttcagcaggg tgcgggtttc
     5281 gccggggccg tcgacgcctt cctggcagcc gataaagtcg cggaagtatt ccgagacttt
     5341 cttgccattc ttgcccttga taaacgatat gtactgcttg gaattggcgt tgttgcgcca
     5401 ctcggagatg ttgatgcgtg cggccaggtg cagctggctc aggtccaggt gcttggcggc
     5461 ggtcacgtcc agcgcatcgt tcaccgccac gccttcgctg tgatgcagca gggcgatcac
     5521 caggtaatcg gtcatgccct gctggtagtg ggcgaacaac acatggccgc cggttgacag
     5581 gttagattct tccatcagct tctgcagctg ttccacggct ttgcgactga acgcagcgaa
     5641 gtcctggccc tcgtccaggt agtgcttcaa ccagccgcta aacgggtatg ccccggactc
     5701 ttcatggaaa aagccccagg ccttgccttg cttggcgtta tagctctcgt tgaggtcagc
     5761 gagcatgttc tcgatggctt gcgaggcggc cagctcggtg tcccgggcat gcagcacggc
     5821 gggcgtgcca tcgggctttt tgtcgatcag gtggacgatg cagtggcgga tcggcatggg
     5881 gttaggtctt caggtcagtc gcagggggtg atggctcctg ggctgctggc gagggcaaca
     5941 cggaaccgat ctacaaaatg tgcgcagagt ttacctgagt agaggtttct ctccatcggt
     6001 cagcgcgtta tgccaggcag ttaacggcct cggtgatgca tctctaagct cagtaaacga
     6061 tggcaaaatg ctggcaatta acgcttatat tcgtcgataa gttcttattt tcaataaaat
     6121 tgtcggtgtc ggttcgcgca gatgaatatc cccgtctagg ttatgtagcg tccacacgac
     6181 gcagtaaagg gacctatcgc ggcggtgtca caagcgctgc cgcacctaaa aactccatat
     6241 atggattgga ggcagcatgc ataacaagat tgtgaaaagc ggtttggtta ttggaagcct
     6301 actggcggcg atctcgggtg aggtgctcgc ggagtcgaac aaaagtagca gccaactgtt
     6361 caatgcgttt tggagcccgg cgcgcttggg ccctctggaa tgtcgttttg gcttgctcaa
     6421 cagcacgaca ttgggcctgc cgcgctgtat gcaggcgagc aataccgcca ttcacctgca
     6481 ggtgttcaag gacattgccc aagccaatgg cggcaatcgg gccgcaggct tgcccggtta
     6541 ccagcgctca atcgactacg tgaaatccac cctggaaggt gcgggctatc gcgtagaaat
     6601 tcagccattc ccctttactg cctattaccc gcaagggccc ggtgagttgc aggccttggc
     6661 gccgacgcca gcggtgtttg agtgggaggt ggactttacc tacctgtcgc aaaccgagga
     6721 tggtgacgtc agtgcgccgg tggtggcggt ggatatcgcg cttggtgcgg gcaacctgtc
     6781 gagcagtggc tgtgaggcgg aggactttgt cgacttcccc tccggttcca tcgccttgct
     6841 gcagcgcggc acctgtaact ttcagataaa ggcagagaac gccgcagcgg ctggtgcctc
     6901 aggggtgatt atcttcaacc agggcgacac cgaggaccgc aagggcctgc ttaatgccac
     6961 cctgggcgac agctatgccg gcggtgtccc ggtgttcttc gccacctacg atgtcggggt
     7021 tggcctggcg cagagcacag aaacccaggt gcgcatgctg gccgatgtgg tgcgcgagcg
     7081 cacgcggacc ttcaacctga tcgccgagag ccgccacggc aatgccaaca atgtggtgat
     7141 ggctggggct cacctcgact ctgtgtatga aggcgcaggc atcaacgaca acgcatcagg
     7201 cagtgccgcg ttgctggagc tggcgttgca gctgcagaaa gtacggccga agaacaagct
     7261 gcgcttcgcc tggtggggtg ctgaggaatc agggttggtc ggttcgactt actatgtcag
     7321 ccagctgccg gacgagcaga agagcaagat caaggcgtac ttgaacttcg acatgatcgc
     7381 ttcgccaaac ttcgcctact tcatctatga cggcgacggt tctgcgttcg gtctgcaagg
     7441 cccacccggt tcggcagcca tcgagaagct gtttgaggag tactacaaac tgcgcggttt
     7501 gccgtccgaa ggcgatgaga tcagcttccg ttcggattac gcgcagttct tcctcgatgg
     7561 catcgccttc ggtggtttgt tcaccggggc cgaggtgctg aagacgccgg aacaagcgga
     7621 cctctatggt ggtactgcgg gtatcgcgct tgacccgtgc taccacgcca gttgcgatga
     7681 cctgaccaac atcgccgagc gtgccttgga gatcaatggc gatgccatgg cctttgtcac
     7741 cagctggtta tcgacttcga ccaaagtggt cgatgaccag atcgaagccg ctacggccga
     7801 gaagagtgtg gcgcgtagtg cgcagagcta cgacatcacc cactggggta aacactggat
     7861 caagtgatcg gctgaaaatg caaaaccccg ccagggctca gctctggcgg ggttttcttt
     7921 gtcggcagta agtgtgggtg gaggcttgtg ctgcgggctt agctggccac caactggcgc
     7981 tgcttgagcg catgcaggcg ctcaatcata tagcgcaggt gcatatgcag ctgatacagc
     8041 tcgctggagt aggacagcgg cacttcgacc cgcgccagtt cgtcttccaa acgctccagg
     8101 cgggcgatct ctgcgtccag gtcgctgggc agcttgccct tgtacagctg ctgatcgatc
     8161 tcgcgcagat gcttgtacca gcggtagata cgcgcgcgga tgcgccactg gtagatcggc
     8221 cccacggcct tgaacagcgg gaacagcagt actagcagcg ggatggccag gatgatgtag
     8281 cggtcggcca gtgaggcgat gcgaaacggc aggtagcgct gcaggatcgg cagacccttg
     8341 tcgtagtagt aatcggcctc ggccagggtg ttgagtgaca gcggtggttt ggccgggtag
     8401 ctgccggccg gatcgagcag gctgccgttt ttcagcacgg ttttcgccgc ttcgagaatc
     8461 agcggtgtga gtgatgggtg gaattcttcg ccggcgacca gggtggagac cgggccgatg
     8521 gtgacgatgt cgcgatccgg ggtgttctgc gccatatcga gcatgccttc gcccacctgc
     8581 agctggctca ggtacggcag gcgcgccaga taggcctcgg tgcggcgcag gctgaccagg
     8641 cgcaccttgg gttccgccgc caggcgctga atcagcgcat tttccgccgg gccaataaag
     8701 aaggccgcgt ccagcttgcc cgcaatcagc gcatcggcgg cgctgctgcc acccaactgc
     8761 tgccagcgct tggggtactg cgcaggttca attcgattgg ccgcgaacaa ggctgcagtc
     8821 accgcctggg tgccgctgcc ctgggaaccg acagccaggc gcagtttcat cagatcagcc
     8881 aggcgctcga atttgacctt gtcaccgaga aacagccaca gcggctcgcg gtacatcacg
     8941 cccaggccat tgagcttgcc gcgcgcttcg tcgctcaggg tcagctcctg gccgctctgc
     9001 accagcgcca gttcaacctc accactgccg agtttgccca ggttgtcttc agagcctgcg
     9061 ctctcaacca gctccaggct aaaaccctgc agttccagtt cagcctgcag gcgctcgcca
     9121 aacgcatggt agccaccgcc tacggcgcca gtggccatcc gcgcatgcat cggtggcggc
     9181 ggcgcggcga aatagaacag cgcgccaacc agccccgcaa ttaccggcac gatccacagg
     9241 ttggtaagga tcaggatttt caggtcatga agaatgcggc gcatggtcag tccttgtcat
     9301 taacttggct gaggatagcc gcaatgactg gccaagctaa atgccttgcg tcttcggatc
     9361 agctgcttac gccagccagg cgcatgctgc gctgctcgcg ccagcggtct agtaacccga
     9421 gcaccaccag caagcccagg gcatacccgg catccatacc gagcatcagc agcaccaggg
     9481 cgcacagtag gcagctgact gctgccagcc agcgccagat gccgtgtagt agcaccgcgc
     9541 ctgcggccat gctcagcagg tagataacga cgaaattgcc attggcgtag cggatcaggt
     9601 catccacggt caggtcgagc acgctggcca ggccggtgca cagggcgcag gcgattatca
     9661 ccagcagcag ggcgcgggcc ggtacgccgt gctggttgcg ctgggccagg ctggcgggca
     9721 acttgccctc gtcggccagg ctccagatca accgggcgaa gccctgcata tagacgttca
     9781 tcgaggcgaa gcaggccagg taacccacag tggcggcgat ccagcgcgct tggtcgccca
     9841 gcagcaggtc gagcattcgc ggcatggatg cagcatcggt ctgcacatcg ccgtaggtat
     9901 gcaggcttag caccgccacc gaacaggccc agtacaccag gccagccagc agcacgccaa
     9961 acagcagggc aagggggaag tcgcgctcgg ggttcttgaa ctcctcgccc atatgggtaa
    10021 aggcttcgat gccgacaaaa caccagaaca tcacccccag cgccgccggc aacaggtgcc
    10081 aagaaccatt gataggtggc agcagtggct ggctgctgac gggcagatcg ccgacccacc
    10141 agatcagcgc aatggtggcg ataattgcca cagcgatagc gccctgaatc atcccgctgg
    10201 ccttggccgg gcgctggccg agtagcagca tggcggcgag ggtggtcagc tggattgcca
    10261 gggcttggcc gcgactgaga tcgaacagcg cctgccaaaa cccgctggcg atatttagcg
    10321 ccgccggcaa acccaccggc agcacagcga gaaacagcag cgcactcagg cgctccatcc
    10381 gcgcgccaaa ggcgcggcca atcaggtgcg gcgcgccgcc ggcatggggg aagtggcggc
    10441 ccagctgagc gaaggtaaaa gccaccggca gcaccagggc aatcagcagc atccaggccc
    10501 ataacgaggc ctcgccggca gcggtggcgg ccagcgccgg gatcacgaaa atcccggtgc
    10561 ccagcagcga ggtacttagc aggccaatcc cttgtagcag gcccaattcc tggttcagac
    10621 ggctcatgtt gtatgctctg cggtttcgtt cgccatgtta aatcgcccgt ctgttacagg
    10681 ctgctggcga agcgtcggct ttgaccgacg atctggcgca tttggccgtc attctgtttt
    10741 ttgcctgaga gttgccgtgg acaagttcga tcagcagata ctcgccctgt tgcgcgccga
    10801 tgcgcgtatg cccgtcagcc agatcgcccg cgaggtcaac ttgtcgcgtt cggcggtcag
    10861 cgagcgcatt cgtcagctgg agcagggcgg cattatcagt ggttatcacg ctcaggtcgc
    10921 ctcgccgggt gatgcggcga tcaaggtcta tctggaactg ttctatagcg gcgggcgctg
    10981 cgagcattat gtcgagctga tgcgcgtgtt ccccgaagtg cgccgttgca gcggtatcag
    11041 cggcgagacc gatatgctgg tgtatatcga agctccgtca atgcagcgct tcagtgaggt
    11101 gcgcggcgag atcgagaact tccccggtat gcagaaagtg aagacccata tggtggtcaa
    11161 ggactggcag ttttgactca gcccatgcgc attctgcaca cctccgactg gcaccttggc
    11221 cagcacttta tgggcaagac ccgtcaggcc gagcaccagg ccttctgcgc ctggctgatc
    11281 gagcaggtgc gcacgcatca ggtggatgcc gtgctgatcg ccggcgatat cttcgacacc
    11341 ggcgcgccgc ccagctacgc ccgcgagcaa tacaaccgct ttatcgtcga gttgcgtggc
    11401 accggctgtg agctggtggt gttgggcggc aaccatgatt cggtggccat gctcggcgag
    11461 agtaaaatgt tgctggcgca attgggtact cgggtaattc cgggcgtctg cgaacagctg
    11521 gatgaacagt tgctggtgct gcaccgacgt gacggcttgc ccggcgcgat tctctgtggc
    11581 atccccttta tccgcccgcg cgatgtattg cttagtcagg ctgggcagag cgcccaggac
    11641 aagcagcagt cgctgcaaca ggcgatccag cagcattacc aggacctcta ctccctggcc
    11701 gaggcgaaac gtaccgagct gggtggcaac ttgccgatta tcgccagcgg ccatctcacc
    11761 accgtgggtg ccagcgccag cgattcggtg cgcgagatct atgtcggcag cctggaggcc
    11821 tttcctacca gcgcatttcc gccggcagcc tatatcgccc tggggcatat ccatcggccg
    11881 cagaaggtcg gtggcctgga gcatatccgc tactgcggct cgcccattgc actgagcttc
    11941 gatgaagcca ggcagcagaa ggaagtgctg ctggtggagc tggatagcag tggcttgcgc
    12001 cagatcaccg cgctgccggt gccgcggttt cagccgctgc tgtccttgcg cggttcgcta
    12061 aaagagctgg aaatgcagat caagcaagcg gcggagcaag gcaatgccga gcaaccggta
    12121 tggctggagg tgctggtcgg cactgacgat tacctgagcg acttgcagct gcgtatcgct
    12181 gccctgtgtg agggcttgcc ggtggaagtg ctgcgtatcc gtcgcgagcg cggcaatgcc
    12241 agcgccagcc tgcagggcca ggccaaggaa accctggatg agctaagcgt ggaggacgtt
    12301 ttcgcccagc gcctaagcag tgaaagcctg gacgatgccg agcaggcgcg tctgctgggg
    12361 ctgtatcagc aggtggtcag tgagctgcgc gagggtgagg tatgaagatc cttagcctgc
    12421 gcctgaaaaa ccttaactcg ctcaagggcg aatggaagat cgacttcacc gccgagccat
    12481 tcaaggacaa cggtctgttc gccatcaccg ggccgactgg cgcgggcaag accaccttgc
    12541 ttgacgccat ctgcctggcc ctgtaccacc gcacgccgcg gatgaacacg gtatcggcca
    12601 gcgccaacga gttgatgagc cgacataccg ccgactgcct ggccgaggtc gaattcgagg
    12661 tcaagggcgt gggctatcgc gcgttctgga gccagcgccg cgcccgcgat aaggccgatg
    12721 gcgcgttgca ggcacccaag gtcgaactgg ccgctgccga tgggcagatc atcaccgaca
    12781 agatcaatga gaagctgcgc gagaccgagc gcctgaccgg cctcgatttc gagcgtttta
    12841 ccaagtccat gctgctggcc cagggcggtt ttgccgcctt tcttgaagcc aatgccaacc
    12901 agcgcgccga actgctggaa gagctgaccg gcaccgatat ctatgggcag atttcccagc
    12961 gggtgtttga gcagtgccgt gaggtgaaaa ccgccctcga tcagctgcgc gccaaggccg
    13021 aaggcgtcga gctgctaaac gaagagcagc gtattgcact gcaagcgcag gccgcgcagc
    13081 tggctattga tgaagcagcg cttagcacac agcaagccga attgcagcgc cagcgccgct
    13141 ggtgtgagga cctgagcaag acccaggcac agttgcacgc cagccagcag gccgagcagg
    13201 cggcgcagca ggatttacag ttggcccagc cgcagctggc gcaactggcc gccagcgagc
    13261 cggcactgcg cttgcagccg ctgcaccatg actggcagca ggcccggcag agcctggcgc
    13321 agaccgagca aggcttacag cagaatgccg ccgagcagcg ccaggctgag caggccattg
    13381 ccgagcacag ctggcaggcc cgcgaactta gcgccttgct gcttgccgat tgccaggcgc
    13441 agcgccagca gctggccgag cgcctgcagc agttgcagca acaactggcc gcgcagccgc
    13501 agcatggccg cctgggtgag cagctgggca gttggggcga gcagcttaag gcgcgccagc
    13561 tacaggccga ggaaatcgcc cagctgcaac tcagtcaaca gcagctgcaa gaaaccatcg
    13621 ccgcgtcgca gacgcaactg aaccagcaaa ctgagcaact tggcgccgaa cagcttcgtc
    13681 tgcagcaggc ccgcgagcag caagacgccg cacagcagca acgcgatgaa ttattggttg
    13741 ggcgcagcga agctgccctg cgtgagcgct ggcagcagct gcatatccag ggcggtgtgc
    13801 tgacccagtt gggcgagttg gtcacggtcc aacagcgcct ggcggccgag cagagccagc
    13861 tgacaacagc cctgactagc ttgcgtgagc agcatcaggc caagggtgcc gaggtggcgc
    13921 agttgcgcct gagctacaag gacctgcagc agcaggtgca ggcccaggaa aaactgctgg
    13981 agcaggaaca gcgcatccag gccctggaga gccaccgtgc ccagctgcaa cccggcgagg
    14041 cctgcccgct gtgcggctcc gagtcgcacc cggcgatcag cgcctaccag gccctggatg
    14101 tctcggcgac caaggcgttg cttaacagca agcgtgccga gctggatgct cttaccgaac
    14161 aaggccggca actggctggc gagctggaaa aactcgccgc gcgcctggaa cagcaacagc
    14221 agcagtttga gcgtggtcgg cagacccagg ccgagcaggg tgagcgctgg caggcgctta
    14281 gcgcgcagtt ggaaaccccg ttggccgatg ccgttgccct ggctaagcag caggcgcaac
    14341 agaacattga acttcaacaa ctgcagcagc aacttgagca gctgcaacag ctcaatacca
    14401 ttcttgagca gcgccagcag gcgcggctaa atctgcagca gcgctgcagt gaactggagc
    14461 aacagcaggc gctcaatcat ctgcagctgc agcaacgcca ggagcagact gagcaaggcc
    14521 ggttacgtct gcagcagctg caggacgttg cgcacaagcg caatgaaagc ctgagccagg
    14581 acctggccga gttcggctat cagctgccgg acgacgccag cgcctggctt gccgagcgcc
    14641 aggccgagtg gcagcgctgg caacacaatc agcagcacag cctggattta cagcgccaac
    14701 tcagtgagct ggaccatggc ttgcaaaatg ctgctcagct ggctcagctc tggcaacaac
    14761 gctggagtgt gctggatctg gccgatcaac cggccttggc caaacccgcc gatgctcagg
    14821 ccgccttgca gcaggcgcag gtgcagctgg aagaggcaca gcgccgtcaa cagcagttgg
    14881 ccggtagtcg ccaggccctg gccagccgtc tgcaggatga acaggcgcga ctgcaacagg
    14941 ccgaacagca gtggacaaag gcgctaagcg ccagcccctt taatgccgag gccgattatc
    15001 tggctgcctt acttgatgaa gctcagcgcg tcgcattgca gcaattgcgt cagcgtctgg
    15061 acaaggctct gagcgatgcc caggccctgg ccagcgccgc cgcgcagcag ctggccgcat
    15121 tgcaggctag cccggccacc gcacttagcc ttgcgcagct ggacgaacag ctgctcgtcc
    15181 ttaacaccca gctaaaagcc ctaagcgaac gccagggcga aatccgcgct cagctgcagg
    15241 gcgacgacgc gcgcaggctc ggtcagcaaa gcctgttcgc tgcgatcagt accaagcagg
    15301 gtgaatacga cttatggcag caacttaaca gtctgatcgg ctcggccgat ggcgccaaat
    15361 accgcaagtt cgcccagggc ctgaccctgg atcatctggt ctacctggcc aaccagcagc
    15421 ttgagcgttt gcacggtcgc taccagttgg cgcggcgcag cagcggtgaa ctggagctgg
    15481 aggtgatcga cacctggcag gccgatgccg cgcgcgacac caaaaccctg tccggcggcg
    15541 agagctttct ggtcagtctg gcgctggcct tggcgctgtc cgatctggtc agccataaaa
    15601 ccagcatcga ctcgctgttt ctcgatgagg gcttcggcac attggacggt gaaaccctgg
    15661 aagtggccct ggacgccctg gacaacctga atgccagcgg caagatgatt ggcgtgatca
    15721 gccacgtcga ggcgctcaag gaacgcatcc cggtgcagct gaaagtgcat aagggcgtcg
    15781 gcatgggcta tagcggcctg gataagcgct ttgcggtgaa tacacgggac aattgatctg
    15841 cgccagcgtt gctttggcca tgcctaccta tactgaaaaa aacgctctcc actgtctcgg
    15901 agcattgcac ttggccaagg tattgatctg cctgctgtgc tgctattgcc tggttgttga
    15961 tgtccatgcg gccccgcgtt cggccgaggc cttgaccctc tgctatgagg atcagaactc
    16021 ctacccctgg gtgatgaccg acggcggcgg gctcaacctg cagttgctgc gtctggtcgc
    16081 cgatgccttg ccgctgcagt tcagcttcgt tgccgtcccc tggaagcgct gtttgtctgg
    16141 catggctcaa ggcacctatg acggcgcttt tgcctccagc ttcaaggagg agcgtctgct
    16201 tctcggccgc tacccgcagg atgccgatgg tcgcctggat gagcgcaaac ggctgcacac
    16261 ctctatctac gccctgtacc ggcgtaaggg cagcccagtg agctggaacg gcgaggagtt
    16321 tcgccagctg cacgggcgag ttggctccct gagcggcttt tcgattgtcg actttattcg
    16381 cgcccagggt gccgaggtgg atgaaaccag ccgtgatccc ctggcactgt tgcagatgct
    16441 taggcataaa cgtatcgaag cggcggcgct gcagagcctg cgcggtgact ttgtgctgca
    16501 gagcaaccct gagctggcgg cacggttgga gaaggtcaag ctgccgctgg aagagaaggc
    16561 ttattacctg atgctgtcca acgcctatgt ggcggccaac ccggagcagg ctgcgcgcat
    16621 ctgggatgaa atcgaacgtc agcgcgagtc gttgacgtat cagcagcagg tgcgggattt
    16681 tctggagcgc tcgcagccct gagctggtta gtgcttggtg cctgccaaac gcaggcgcat
    16741 gatcgtgctt cgcctcgatg agtattgctg catggccaag cctcccgccg ctccaccgtc
    16801 tgcctctgcg cttgggcgtt ttcttacgtt ggcgggcacc gctacgcgta tcggcggcag
    16861 cgtgcttggg cagagtttgc ggcctgggcg cggcggaatt gattggcagc cagtgggcga
    16921 tttgctcacc gatgtgctgg gcgagatgaa aggcccggtg ctgaaactgg ggcaaatggc
    16981 ctcgcaatgg cagggggtgt tgcccgaacc ggtagcgctg gcgctggcct cgctgcaaaa
    17041 ccgcgtaccg gccttgccct tcagcgcgct gcgcgggcat ttgcagcagg tgtatggcgc
    17101 ggaccttggg cagttttttc agcagatcga cgaacaaccc tttgccgctg cgtccctcgg
    17161 ccaggtgcat cgcgccatag cggtggatgg ccgggcgctg gtgctcaagg tgcaataccc
    17221 gggcatcgcc gaaatctgcc aggcggatct gcgtcagctg cgccgtttgc taccgctggg
    17281 gcgcttgttc cgcgcgcccg ccgagcagct ggaaggcctg tatcaggagt tggcgacggt
    17341 gatcgaggcc gagctggatt acccggcgga gatgcgccgg ctgcaggatt ttcgcgcgca
    17401 tttcgccgat tggccgggct tgcgtttgcc gcagccgcag gaagacctct gtcgccccgg
    17461 tgtgctggcg ctcagcgagg aggccggctt gccctttgcc gaggtcagcc aggccagtgc
    17521 cgaggtgcgt gagcgtctgg ccctgaccct ggtgcgttgg ctgagtgccc aggccttcga
    17581 gctgggcctg ctgcatgctg atccgcatcc gggcaatttc gcttataccg ccgccggtga
    17641 gctggtggtg tatgacttcg gctgtatgca ggcgctgtcc gcgccgctac tggcggccta
    17701 tgtgcaaacc tacaaggcgt tgcaggcccg cgatggcgag cagctggaaa aggccttcca
    17761 ggcgctcggc acacgccagc cgaaatccac cacgccctat ggtttgtacc gccagctgca
    17821 tggtttgctc gggccgctgt tgcagcctgg tgtgcactgg gactttgccg ccacgccgct
    17881 gcatgagcag gtgcaacggc tgttgcccga tgtgctgggt gccttgggca gcctgcaacc
    17941 ggcaccggcc accttgctgg tcaatcgcac gctggaaggg cattactgga acctcagccg
    18001 cctcggtgtg gcgctgccgg tggccgatct gttgcaggtg cagctggctg ggcgttaacg
    18061 catggtctta acgggtacgg cttggccatt ttcgatacgg gtcagaaaga ctgcgctgga
    18121 gccctgatgc tgactgcgcg agaacgccac cttaaaaccg cccaggtccg tttcgaggtg
    18181 ctccaacgca tcgagaaacg cctcacgcgt cggctttgcg ccggcctggc gtagcgcctg
    18241 agtaaacacc gccgcgccga tataaccctc cagcgaagca tggtcaaaat cgccagggcg
    18301 catgtcgatc tgataggccc tcacgagagc cagcgagctg tcgtgcgggg agggcaccac
    18361 ctgggtgata tagacgccgt caccgtcatt gccgacttcg cggataaacc cctcggtgcc
    18421 gataaacgac acgctgataa agcgcgtatt gaagcccagc gcttttgcct gcttgatcgc
    18481 ggccgccagt tgcctgtacg tgccgacaaa gaacaccgct tccggctggg cgcgctgcag
    18541 cgccttgata gcctccgtca cgtccagcga attacgctgg atgcgcgcct gcgcataaat
    18601 cttcaggtcg cgcttgagca gcgcgccgtt gaggccgccc ttgaccgact cgccgaagga
    18661 gtcatcctgc atcaggatgg cgatcttgct cagctttagg tcctgggtca cgcgctcgac
    18721 cagttgctcg gtttcctcga tataggaggc gcggatgttg aacacccagg attttaccgg
    18781 cgtgcgtaaa aactcggcgc cggtaaaggg gaacaggtaa ggcacctgcg cccgcaaagc
    18841 caatggcacg gcggcgcgtg aggtgggtgt gccgacgtaa ccgagcaggg cgaacacctg
    18901 ttgggttttc agcaggctgc gggtgtgtgc ggcggtctcg ctgggctcgt agccatcgtc
    18961 ccggctgagc aggttgatac gccggccatg taccccgcct tcggcgttga cgcgcttgaa
    19021 gtaggcctgg gcgccattga gcatgcccaa acccagagcc gccgccgggc cactctgggc
    19081 gttgaccata ccgatacgca cttcatccgt actcaggcct ggctccgctg cgctggcaaa
    19141 acccggcgtc agcaggtagc tgagcaccag gccacttagc agttgtcgag aggtcatcaa
    19201 tacgctcctt gtcatgaccg caggccactg caggcctgct cgtccatttc ttattatttg
    19261 gggtgtgcat gtgatcctca aaacactgta gccagctgtg cataaaagac cgtacttact
    19321 cgcttaccgg tgatgctgac ggctccggct ggtacttgcc ttgctgtaga tcatcgaaat
    19381 aacgctgata acgcccgctg tcgcgatagt gttgcaggcg tttgttgaag cgttcgagca
    19441 gcgccgggct gccttccagg cttttcggca gcatcaggta gctgaggttg accagcagtg
    19501 gcttggggtg atgggtgatc ttggcctgat cttccgcgct gaagtggctg cgcagggcgg
    19561 cgtagcccac gttcatttcc tgtgggtaga gcaccacgcg ctctttgagc agcttgtcga
    19621 agttctgctg gtcgttggag acgcgctcaa ttttcacctt gttctcggcg agaaaggcat
    19681 cgaaatccgc gccatagctg tattccaggc cgccgcccag agtcatgccg gtgaggtcgt
    19741 caaagcgttg ccagtcgaat ggcaggcttt ttaggtgaaa gaatacgaat ttttcatcga
    19801 gcagcggcgc gctgaacaga aaatccgcct cgcgctcggc cttgtgcatc cacaccgcgg
    19861 tgccgtcaaa gcgcccggcc gcagcggcag agtaggcgcg tggccagggc agaaattgga
    19921 agctgacgcg atagccctca tcggccagca ggtcgctgat cagatgggca attactccgt
    19981 tgtgcttcaa ctcgctggac aggtagggcg gccagtcacc aacgctgatc ttcagttccg
    20041 gctgttcgct ggcctgcgcg tggagcccca gcatcaggca gagcgcgacg aacaggtttt
    20101 tccacttcat taaaacccct tagtgacaac cgggaatatc gcatacgcgt ggtcatgagc
    20161 cagtaatacc actgcgcagg cagataaaac gccattaatg tttcattaaa acgtcatcgc
    20221 gcgcgtgaag actgctgcgg atttgtccaa tccccgtgtc tttgcataag gatagttgct
    20281 gtgctgcgaa tccctacact ttgtgcgctt tttgccctgg ctcctttggc ctcggccctt
    20341 gagcttaaac ccctggcgct gtatcaaagc ggctttgctg ccggcaccga aattgtcagt
    20401 gtgcaggcct cgagtatgcg tgtgattgtc tccaacagtg ccgaggggct ggtggatatc
    20461 ctgcaactgg acccggccaa gggtctcaat cgcattgccc ggcataactt ggtggcagcc
    20521 ggcacggggg aaatcacctc ggcgattttc catcccagcg aagacctgtt tgccgtgtgc
    20581 atccgcaaca gcgatggcct gaaaaacggc cgcgtcgagt tgcgtgcggc ggccgatggc
    20641 aagttgctca acactgtaga aatcggcatt tggccggaca gcctggcgtt ttcacccaag
    20701 ggtgatttca tcgtggtggc caacgaaggt gaaggctatg tgcgtgacgg cgagagtttt
    20761 cgcagcggtg aaggctcgat cagccttatc gacttgcgcg gtggtgtagc tgcggccaag
    20821 cacagcctga tcaaactgcc ggacctggcg ggtgtcgagg gcacaaccca ggccgagcac
    20881 aagcgtctgc tggagcgggt gattgatggt gaagagctga aagtgccgtt cggcaccagc
    20941 ccggagcata tcgagccgga atacgtgacc ttcagcccgg acggcgcgcg tgcctacgtc
    21001 agcctgcaag agaacaacgc gattgccgtg gttgatgtgc tgcaaggcaa gctggacaaa
    21061 gtatttggcc tgggcaccgt gcgtcatgcg gcggatatcg tcgatgacgg caagtatcag
    21121 ccgactgccg agctgttcgc cctgcgcgaa ccggatgccc tggccgtcag cgccgatggt
    21181 cgttatctga tcagcgctga tgaaggcgat accgatccga aaatcgccaa gaccaaggcc
    21241 ggtctgccca gcggtggcgg gcgtacggtc agtgtgttcg atgccataag cggcaagctg
    21301 ctcggcgata ccggcagcca gctcgatgat atggctgctc aggccggggt ttacccggat
    21361 gtacgcagcc cgaacaaagg cagcgagccg gaaggcgtgg tgagttttga tgcctttggc
    21421 aaacgtctgg cggtagctac tctggaacgt gcggatgccc tggccctgat cgatctcgat
    21481 cagccggagc aacccaaggt gctgcaggta atcggtgccg gcgaaggtgc cggttccggc
    21541 aagctggcgc cggaaggcct ggcgcatgtc gagcacaacg gtcggcattt cctggtcacc
    21601 ggcctggaga agagcggcaa cgtcgcgctg ttcgaattgc tcaagtaata cgcttgttat
    21661 cggtcggacg cgctgcatgc gtctggccga gtaccaaaca cgccggcctt tgcgcctaat
    21721 gctgttcact gtaggtcgca tgctttttac tcccttctcc catttatggg agaggggctg
    21781 ggggtgaggc cttcccctct ccctaaccct ctccccggcg gggcgagggg atgatcgcgt
    21841 ccacccgatg cttaagtgaa cagcattagg cctttgcgcc ggctttttat ggcctgccat
    21901 tcctggcggc cacccttcgg gccgtcgctg cgcgacgttg aaaatcgctc ctggcgcctt
    21961 tttctttgcg gccagttccc cgccggcctc tggcccttta gagtgctgca gcgcaatcgt
    22021 gcaggtatta ggggatggct ggcacatgac cgtttgggtc gtttcacttc tgtttgtatt
    22081 gacctacctg ggcatggccg cagggggcat tcgtggcctg cgtgtcgatc gcagctggat
    22141 tgccggctgc gcggcggtgt tgctgctggt cagcggcgca caatccccgg ccgaggcgac
    22201 cagtcatctg gatgccggtg cgctgttgtt gctgctggcg ctgatgctga tcagtgcgca
    22261 gttcgacttc tccggcgtgt acgcctggct taaccagcaa ctcagccact atgccgagca
    22321 gccggcgctg ctgctgttgg gcgtggtcct gctgggcggc gtcctgtcgg cgttgctggt
    22381 caatgacatc gtcgccttcg ctctgacccc gctgctctgt catagcctgc tggcccgcgg
    22441 cctcgacccg cggccgtttc tgttggcact ggcgctgtca tgcaatgccg gctccagcgc
    22501 cagcctgatc ggcaacccgc agaacatctt gatcggacag gccggtggcc tggatttctg
    22561 gtcctacacc tttatcgccg gcccgcccgc actggcggca ttggccatca cctacgcggt
    22621 gatctggctg cagtggcgca aacgctgggg cacggccaag ccgctggtgc aggcagaaac
    22681 cccggttgag cgtatctacg gcctgcgcag ttacctgaag cccctgctgg ccagcctggt
    22741 gctgctcggg ttgttttcca ccagtatgcc gcgagagatt tctgccctgt tgatcgcggt
    22801 gctggtgatg atctcgcggc gggtggacag ccgcgactat gtgcagaagg tcgactggaa
    22861 tctgttgctg ctgtttgccg ggttgttcgt cgtgactggc gcggcgatga gcctgccgca
    22921 ggtcgccggt atggccggca atctggccga gctgggcctg ctgccgcacg gggtgatcac
    22981 cctggcgagc ctgtcgctgc tggccggtaa cctgatcggc aatgtgccgt atgtgatgtt
    23041 gctgctgggc gtgttgccgg atgccagtga ggccttgctg gtgggcctgg cgatcatgtc
    23101 gacgctggcc ggcaacctgc tgctgatcgg cagcgtggtc aacctgatcg tcgccgaaag
    23161 cgcgcgacgt cagggcgtgc cgctgagctt tgtcgacttt gcccgcagcg gcgtgccggt
    23221 gaccttgctt agcatgggcg tggctgggtt gtggctggcg ttgggcggtt ggttgccttg
    23281 gtagtgtcag ggcacgggta ggatgggtta gccgctgcac acagcctgag tcgtgcgcct
    23341 atatcgttgc ggcgtaaccc atcactgggc gccttaggca gcatgggtat cgctgcgctc
    23401 aacctacgcg gcccgaccca tcctacgacc agcgggcttt gcgccaggcc tgctgctgtt
    23461 ccgccgtctg gaaggtccag gcgacaaagc ggctctgctt ctggccctgg gccatttcta
    23521 cggtttgcac ctgcagcgcg ccggcctttt tcagcgcgct gtacacgccc ggcaggttgc
    23581 cagctttgga aatcaggctg ctaaaccaca gcacctgctg accgacctcg acactttcct
    23641 tgatcaactg gctgacaaac gccgcttcgc cgcccttgca ccacaactcc gccgcctggc
    23701 cgccgaagtt cagcaccggc agtttgcgct tgggatcgag tttgccgagg ttgcgccatt
    23761 tgcgtttgct gccgctgctg gcttcttccg cgctggcgtg aaagggcggg ttacacaggg
    23821 tcagatcgaa gcgctcatcg ctctgtagca agccttggaa gatatgcgtg ctgtcgctct
    23881 gctggcgcag ctcaatcgcc tcgctcaagc ctgggttggc ctgcacgatg gccttggccg
    23941 aggagatggc gctggcggca atatccgagc cgagaaactg ccaggcatat tcgcgattac
    24001 ccagcagcgg gtagatgcag ttggcaccca cgccaatatc cagcgcgcgc accttggccc
    24061 cgcgcggtat ctcgccgcca ttgctggcgg ccagcaggtc agccaggtaa tgcagatagt
    24121 cggcgcggcc ggggatcggc gggcacaggt aatccgccgg gatatcccaa tgatcgatgc
    24181 catagaactg cttgagcagc gcccggttga acactttgac cgccgccggg ttggcgaagt
    24241 cgatgctctc attgccatag ggattgatga tcacaaaggc cgccaactcc tggctggcct
    24301 tgatcagcgc ggggaagtca taacgcccct gatggcggtt gcgcggatgc aattggccct
    24361 tggccgtatc gggcttgcct gcagcaggtt tgctaggcgc agggcgcggt gctgtgggtt
    24421 tacggctggg gcgtttcggc ggcagggaca tggcaggcgg atctaaggct tggggcgcgg
    24481 gattttccca cagctgggcg cggcgtgctt gtcgatagca gtaaatcagc tgcgcggagg
    24541 gttacatctg gcctggcagt ttcgttcagg gataagcgag tacggctttt atctgcggca
    24601 gattggcggc aatccactgc ggattgatcg cgccccagtc acgaatttca tagtgcccgg
    24661 cgttattgcg ttcgccgctt tcctgctgga aggcgcagtc gatatccagt tcggccaggg
    24721 cggtcagggt gtcctgggcg gtgcgccgcg gcataccggt ggcggccatc agcgccggga
    24781 cgctggtggc ggtgccgctg tcgatcagcc aggccacata caggcggcgg tagaagctgg
    24841 tacgggtttt actgacgtcc atgggccctg ttcctggctt gatgccgtgc gccactggcg
    24901 cacggcctga ttgagggtca gctcagtgtg gcaatcgcaa tataggttgc tgcaccggag
    24961 atataaccaa ggaaggccag caggctgatc ttcttcaggt accagataaa gttgatcttc
    25021 tccatgccca ttgccgcaac gccggccgcc gagccgatga tcaggcagct gccgccggtg
    25081 ccagcgcagt aagcgagcat ttcccagaag gtgccgttga cctggaaatt gctcagccag
    25141 gcggtgtcgg caccggccag cgccagggct tccggggtga tcagtgggta cattttcatc
    25201 gcaccagcta ccagcggcac gttatccacc accgatgaaa gcaggccgat ggcaatgttg
    25261 atcgcataca cattgcccag gctttcacgc agcgcgacgg cgacctgggt caggtggccg
    25321 gcagtggcca ggcttgagac cgccagcagg atgccgagga agaacagcac gctggtggta
    25381 tcgatacgcc gcagtacgcc gacaaccgac agcgggtgct tgtcttcgtc gttcttgccg
    25441 cggtgaatca cttcggtgac cacccagagc acgcccaggc cgaacaggat gcccatatac
    25501 ggcggcaggt gggtcacggt cttgaacact gggacaaata ccagggcgcc gatgccgagg
    25561 ataaatacca agttgcgctc gaagggtgtg gtcgggtcgc ggcgctcttc atcggattcg
    25621 ctgagcagtg ggcgctggat ttcgcccttc atggtcatgc tcagcaccag cagcggcacc
    25681 agtaggcaga ccacgctcgg gataaacagg ttggcgatga tgccactggt ggtgacctga
    25741 ttgccgatcc acagcatggt ggtggtcacg tcaccgattg gagaccaggc gccaccggcg
    25801 ttggcggcaa tcaccacgat accgacgtag aaccagcgtt ctttctgatc agcgaccagt
    25861 ttgcgcagca gtgaaaccat gacgatggtg gtggtcaggt tatccaacac ggccgagagg
    25921 aagaaggtaa tcagcgccac gacccagagc agggtgacgc gcttgttggt gcgaatgcgg
    25981 tcggtgatga ccttgaagcc ttcgtgggca tcgatcagct cgacgatggt catggcgccc
    26041 atcaggaaga acaggatctc ggagatttcg cccagatgat ggcgcagctc ttcggtgacg
    26101 tgatggtcat tgccactggc gccggcggtg gcggcaccga tcagcggcag aatgctgtcc
    26161 gcgccgagta ccagcagggt ccagcagatt accgcggtca ggatggccga cgcggcttta
    26221 tcgattttca gggggtgttc gagggcgatg cacaggtaac cgaggacaaa gatcaatgcc
    26281 atcaacgcat acatggtcag aattccattt gcagggcttt tttattgaat tagaggtgcc
    26341 ggaggctttt gacgcgccga cgaagatgcc tgaaatgctc agaaattggg aggggattta
    26401 tcgcgcagtg ggtctgcccg aggcctgtgc agggtttttc tgactgcttg gttagccctg
    26461 gcgcgcccga actgcgcggt gatgcgcagc tgcgggcggc tgtggactta ggcctgcgac
    26521 aggtcgtcgc gttcagcatt ggccggcatg acgggctgcg gcgggaagat aaacgccagg
    26581 cagatcgcca atagcggcat cagcaggttg aagaagcagt acggcaggta actcagggtc
    26641 gccacgccga gcgtcgcggc catataggcg ccgcaggtgt tccacggcac cagtaccgag
    26701 gtgagggtcc cgccgtcttc cagggcgcgc gacaggttga ccggcgccag cccgcgtttt
    26761 tcgtactcgg cgcggtacat gcggcccggc agtaccaggg cgatgtactg gtcggcggtg
    26821 atgatattgg tgccgatggt ggtggcaatg gtgctggcga tcaggctgct ggtgcttttc
    26881 gccaggtgca gggtgctttg cagcaggcgc tggagcaggc cgagcttttc cagtacgccg
    26941 ccaaagcaca tggcgcagat gatcagccac accgtggtga gcatgctggc catgccgcct
    27001 ttggacagca ggccgtccag cgccgggttg ccgctgctgg attcataacc ggcgaacagt
    27061 gcggtccaca cggttttcag cggcgccagg ctgccggccg ccgggtcggc caggcgcgcc
    27121 atcacctcgg gctgaaagat cacggcgaac acgccgccga gcagcgcggc aacaaacacc
    27181 gctggaaagg ccgcccactg gcgcaccgcg agaaacagca gcagggccac cggcagcaac
    27241 aggtgccagc ccaggctgaa ctgagcttcc agcgcctgca gcacctcggc gatgcgcgcg
    27301 gtgtcatggc tgccatcggc gcgctggccg aggataaaga acagcaccag ggcaatgccc
    27361 agcaccggca cagtggtgcg cagcatcagg cggatatgtg caaacaactc ggccccggca
    27421 gcagcgggcg ccaggttggt ggtatccgac agcggcgaga gcttgtcgcc gaagtaggcg
    27481 ccggaaatga tcgccccggc ggtaatcgcc ggatccagcc ccaaaccggc ggccacgccc
    27541 atcagaccga tgcccaaggt gccggctacg gtccaggagc tgccgatgga cagcgcggta
    27601 agggcgcaga tcaggcaact ggtgacgtag aagtattctg cggaaatcag cttgaggccg
    27661 agccagatca tggttggcac cgtgccggcg agaatccagg tgccgatcag cgcgccgacc
    27721 gccagcagaa tcaggttggc cttcatcgcc atatggatgc cggcgaggat gccttcctcg
    27781 atctgcgccc actgcaggcc attcttcaga ccgaccaggc cggcgacaaa ggcggcgctc
    27841 atcagcgcga tctggttcgg cccactggaa gagctgtctc catacaggta aacggaaagg
    27901 cccagcagca gcaccagcac gccaatcggc agcagggcat cgagcaggga gggggagcgt
    27961 gcatcagtca tgggtgtacc ggtcgatcat ataaaccgtg ggaagcgctt cagcggcgac
    28021 agtcgcggct gaagcccctc ccacaaaaaa agaaacccgc gccagtgagg gcgcgggctg
    28081 cgggtgttgc gggggtatca gagtgcggcg atcttgccgc gctgctcaac catcttgctc
    28141 agggcttgct cggcttcagc cagcttggcg cgctctttat cgagcacttc ggccggcgcc
    28201 ttggcgacga agccttcatt gctcagcttg ccaccaacgc gctgcacttc accggagaga
    28261 cgggtgattt ccttgtccag gcgcgccagc tcggcgtcct tgtcgatcag cccggccatt
    28321 ggtaccagca cctgcatatc gccgaccaga gcggtggcgg acattggcgc ttcttcgccc
    28381 gcttccagta cgcgtaccga ttccagcttg gccagcttgt tcagcagtgg cgcgttgtcg
    28441 gccaggcggc gcaaatcttc cgcgctggcg ttctgcagga tgatatcgat gcgcttggcc
    28501 atggagattt tcatctcgcc acggatctgc cgcaggccga gcatcagctg cttgacccac
    28561 tcgatatcgc cttcggcggc ggcgtcgatg cggctttcat tggccactgg ccaggcctgc
    28621 agcatgatgg tctcgccgct gacgccggct tgcgctttga tgcgctgcca gatttcttcg
    28681 gtgataaacg gcataaacgg gtgggccaga cgcagcgcca cttccagcac gcggatcagc
    28741 gtacggcggg tgccgcgctg gcgttcgatt ggtgcggttt cgtcccacag cactggcttg
    28801 accagctcca ggtaccaggc gcagtactcg tcccagataa attcgtagag cgcttgcgcg
    28861 gccaggtcga agcggaaggc gtcgagctgg cgggtcactt cggcttcggt gcgttgtagg
    28921 gcggagataa tccagcggtc caccgaggac agctcgaccg gctcaccgtt gacgcctgtg
    28981 tcctggccgt cggtgttttc cagtacgaag ttggcggcgt tccagatctt gttgcagaag
    29041 ttgcggtaac cctcgacgcg gcccatgtcg aacttgatat cgcgaccggt agaagccaat
    29101 gccaggtttg taaagcgcag ggcgtcggtg ccgtatgcgg cgatgccttc ggggaactcg
    29161 gcgcgggtct gcttggcgat cttctcggcc agcttgggct gcatcatgcc gctggtgcgt
    29221 ttttccagca gctcgtcgag ggtaatgcca tcgacgatat ccagcggatc gagcacgttg
    29281 cccttggact tggacatctt ctggccctgg ccatcgcgca ccaggccgtg cacgtacacg
    29341 gtcttgaacg gaatttgccc ggtcagatgg gttgacagca tgatcatccg ggcgacccag
    29401 aagaagatga tatcgaagcc ggtcaccagc acgtccgtgg ggtggaaggt cttcaattct
    29461 tcggtctgct gcggccaacc gagggtggag aatgtccaca gaccggagct gaaccaggtg
    29521 tccagcacgt cttcatcctg gcgcaggttg gcgtcgccga ggttgtgggt ggcgcgtact
    29581 tccgcctcgt cgcgaccgac atagacgttg ccggcatcgt cgtaccaggc cgggatgcgg
    29641 tggccccacc acagctgacg gctgatgcac cagtcctgga tatcgcgcat ccagctgaag
    29701 tacatgttct cgtactgctt gggcacaaac tggatttcac cgctctcgac cacggcgatg
    29761 gctttttcgg ccagcggctt ggtgctgacg taccactggt cggtcagcca cggctcgatg
    29821 atggtgccgg agcggtcgcc tttcggcact ttcagcgcgt gggcgtcgat gctttccaag
    29881 aggcctgcgg cctcgaaggc ggcaacgatc tgcttgcgcg cctcgaaacg atccaggccg
    29941 gcgtattcgg ccggcaggct gcagtcgatc tcgctgttga cgctgccatc gacattgaac
    30001 gcctggacct gtgcgagcac ggcggcgttc ttgtcgaaga tattcagcag cggcaggttg
    30061 tggcgcttgc cgacttcata gtcgttgaag tcgtgggccg gggtgatctt cacgcagccg
    30121 gtgccgaact cggggtcaca gtaatcgtcg gcgatgatcg ggatgcgccg tcccactaga
    30181 ggcagctcga cgaaggtgcc gatcagggct ttatagcgtt cgtcttccgg gtgcacggcc
    30241 acggcgctgt cgccgagcac ggtttcaggg cgcgtggtgg cgacgatcag gtaatccttg
    30301 ccgtcagcgg tcttgttgcc gtcagccagc gggtaacgca ggttccacag gctgcctttc
    30361 tcgtcgtggt tttccacttc gaggtcggag atggcggtgt ggaacttggt gtcccagttg
    30421 accaggcgct tgccgcggta gatcaggccg tctttatgca ggcggacaaa ggcttcttta
    30481 accgattcgg acaagccgtc gtccatggtg aagcgctcgc gcgaccagtc caccgagcta
    30541 cccaggcgac gaatctgccg agtgatagtg ccgccggact gctccttcca ttcccagact
    30601 ttctccagga atttctcgcg gcccagatcg tgacggccaa tgccgtcggc ggccagctgg
    30661 cgctcaacca ccatctgggt ggcgatacca gcgtggtcgg tgcccggctg ccacagggtg
    30721 ttgcggccct gcatacggcg gaaacggatc agcgcatcca tgatcgagtt gttgaaacca
    30781 tggcccatgt gcagactgcc ggtgacgttc ggcggcggga tcatgatggt gtaaggctca
    30841 cctgcaccct gcggggcgaa atagttgttc gcctcccagt tctggtacag ggcggtttca
    30901 atggcgtgcg gctggtaggt cttgtccatg cgcggcggga cccttatgac ggctagacgg
    30961 aaaagccggc aagtatagcg gggatggcct gtgtgacgta agggcgcgcg tggtgcggct
    31021 gactggtggg cgtccgcgtg gttacggtcg acgcggcggc agcaagcggc ttaggcgcgc
    31081 atccaggcgg cgttttagct cggcctcgat ctgcggcacg aaatcgtcaa tcacatcctg
    31141 caggatcagc tgagcggcgg cgcgcaactc gctatccaga cggttcatct cgctgtcgcg
    31201 gctgacgctc tggctgaccg tttcacgcag ggtttgcgca actggcggtt tgggtgctgg
    31261 cgcggcggtc acaggtggcg gggtgacgat ttctgagagc agggggatgc tgtccggatc
    31321 gagcgagtcg atcagtagtg gcggttcgag gttttcatcg cccagcagtt ggcggatcga
    31381 ctccaggtcg ttcagcagtt gagcgggttt ttgcggcggt tttggggtgt ccatggtgtg
    31441 caactagagt tcgactcgtt ttggatcata cccgcgctgg cgatagctgc ggaaattctc
    31501 ccggcaggcg gtcagcagat cgggttcctg gttgacgatt tcaatcaccc ggctaaagcg
    31561 ctcgacctgc ggtgaaaggg tgctgccgag gttgatcaat acaccttggg cgctgctggg
    31621 ttcttcgtcc aggccaatca ctaccggtgc ttgcgcgtca tcctggtaga ggctgtgcgg
    31681 cacaaagctt tcagccttga aacgccacaa cagttcatcc acttcggtgc attgctgcgc
    31741 atcgctgccg cgcagaaaca ccggcaggcc ggcgcgccag gctttcatcg ccaactgaca
    31801 ggccgcgcgc aggcgctcgg ccggcacggc ggtggagagt acgtagaatt ctattcgggg
    31861 catgacggca cgctttggcg ttagttgcag gagcggcttg ctgacaggcg cgcagcttac
    31921 cagtcaatcg cgctcaagac cgctcctgca atgcgccaga ggctcaggcg ttgctgcgat
    31981 ccagcaagta ctgcgtcagc atcggcaccg gacgaccggt cgcgcctttg tccttgccgc
    32041 cgctgatcca ggcggtgccg gcgatatcca ggtgcgccca gtggtagttc ttggcaaaac
    32101 gtgaaaggaa gcagcccgcg gtgatggtgc cggctttcgg cccgccgata ttggcgatat
    32161 cggcgaacgg gctgtcgagc tgttcttggt actcgtcata cagcggcagc tgccaggcgc
    32221 ggtcgtcagc ggtaaggccg gcgccgagaa tctgctgcac cagttcgtcg ttattgccca
    32281 tcaggccgga gacattgctg cccagggcca cgatgcaggc gccggtaagg gtggcgatgt
    32341 cgatcactgc ttgcggcttg aaacgctcgg cataggtcag ggtgtcgcac agcaccaggc
    32401 ggccttcggc gtcggtgttg aggatttcca cggtctgccc gctcatggtg gtgacgatgt
    32461 cgcctgggcg agtggcgccg ccactcggca tgttttcggc acaggccagc aggcacacca
    32521 ggttgattgg cagttgcagc tcaagcacgg ccttgagggt gccgaacacg ctggcggcgc
    32581 cgcacatgtc gtacttcatc tcatccatgc cggccgccgg cttgatgctg ataccgccgg
    32641 tgtcgaaggt gatgcctttg ccaaccaggg cgtagggctt ctcggttttc ttgccgccct
    32701 gatagttgag cacgatcatc cgtggcggct ggtcgctgcc ctgggcgacg gcgaggaagg
    32761 caccggcacc gagttccttg agtttcttct cgtcgaggat ttcgaccttg aggtttttgt
    32821 aggccttgcc aagatttttg gcttcttcgg cgagatagct tgggtggcag agattcggcg
    32881 gcagattacc gaggtctttg gtaaaggcca tgccagcggc aatcgcctgg gcgtgcgtgc
    32941 tggcgcgctc ggcgtcggcc aggttggctt tttccgtgag cagggtgagc ttcttcagtg
    33001 cgccggcttc ggccttctgg ctcttgaagc ggtcgaagca gtagccggcg tcggccagac
    33061 tttccaccat caggcgggtt ttgccgtagg tttcgcggcc cttgacctgc agctcgtcga
    33121 gcgcgagtac ggcgtcgttg cctccgaggt ttttcaggct gctgtaaacg ctggtgatca
    33181 atttgcgcag ttggcggtca gacagttcgc tgtctttacc ggtgccgacc agcagcacgc
    33241 gttcggcctt caggttcggc aggttgtgca ccagcagggt ttggccgact ttgccagcca
    33301 ggtctccgcg cttgagcagg gcgctgatcg caccagcgct ggcggtgtcg atggccttgg
    33361 cggtttcgcc gagttttttg ccctcaccaa cagggataac cagggtggcg gtttttagtg
    33421 tttctgggcg tgcgctttta acaataaatt ccattacgtg gtgtccccaa gac
//
LOCUS       DASRUM010000165        13034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_747459, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000165 DASRUM010000000
VERSION     DASRUM010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 13034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13034
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747459"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..90)
                     /locus_tag="VFF22_10805"
     tRNA            complement(16..90)
                     /locus_tag="VFF22_10805"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Gln,seq:ttg)
     gene            complement(134..1009)
                     /gene="ispE"
                     /locus_tag="VFF22_10810"
     CDS             complement(134..1009)
                     /gene="ispE"
                     /locus_tag="VFF22_10810"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HZX17341.1"
                     /translation="MSAPAIPAAAELILPAPAKLNLMLHILGRRADGYHELQTLFQFL
                     DFGDELGFALRSDGEIHLHTPIDGVPHDSNLIVRAARLLQQHANCTLGADIWLDKRLP
                     MGGGIGGGSSDAATTLLGLNYLWKLKCSEDQLATLGLSLGADVPVFVRGHAAFAEGVG
                     EKLQPVTLREPWFLVAIPQVLVSTAEIFSDPELTRDTPPIKVRSLLEGGGRNDCQPVV
                     TKRYPEVRNALILLNKFVQARLTGTGACVFGSFPNQDDADKVARQLPATLPSFIAQGR
                     NISMLHRKLETLAGK"
     gene            complement(1006..1623)
                     /gene="lolB"
                     /locus_tag="VFF22_10815"
     CDS             complement(1006..1623)
                     /gene="lolB"
                     /locus_tag="VFF22_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011335924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HZX17342.1"
                     /translation="MFARHLIVFSLITLLAGCAGLTSREALEGQGDAQSWQAHKQQIS
                     NLDAWQINGKVGIRAPKDSGSGTLFWLQRQDYYDIRLSGPLGRGAARLTGRPGAIQLE
                     VANQGRYQAESPEQLLQDQLGLNLPVSHLLWWIRGLPSPDSKSRLNLDSQSHLAQLSQ
                     DGWHVEYLSYAEQNGFWLPERIKLSGFDLQVTLVIKDWQPRQLGQ"
     gene            complement(1623..3353)
                     /locus_tag="VFF22_10820"
     CDS             complement(1623..3353)
                     /locus_tag="VFF22_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZX17343.1"
                     /translation="MNRSFALLTAAALLGGCQTFAPSAPDGTPPVEDPAVVTTETQPQ
                     VYRNFSQETLLSLLIAELAGQRNRFDIALENYVQQANATQDAGVAERGFRIAEYLGAE
                     QPALETSLLWAKTSPENIDAQRAAAVQLARAGRYDESMTFMEQVLQRQGDTHFDFLAL
                     SAAETDPDTRAGLLQSFNRLQAKYPDNSQLQFGKALLLQQDGRQQEALELLEQQPSKD
                     RPIASILLHARLLQSLERNDDALPLLEKGIEQHPDDKRLRLTYARLLVAEDRLDDAKG
                     EFASLLQQNPNDDDLRFSLALVCLEAKAWKEAIVYLEELVERGSHVEAAHYNLARAYE
                     ELGDTPSALIEYALVGPGNDYLPAQARQSDLLFKEQRGEEASERLLQARESQPDYAIQ
                     LYLIEAEAWSKQQNTERAWQLIQQALEQFPEDLNLLYTRAMLAEKRDDLALLERDLRF
                     ILEREPDNAMALNALGYTLADRTTRYAEAKQLIEQAHQLNPDDPAVLDSLGWVNYRLG
                     NLDEAERLLRQALQEFPDHEVAAHLGEVLWAQGKQKEARKLWREALKQQPDSTILRST
                     LLRLTGAETL"
     gene            3538..4833
                     /gene="hemA"
                     /locus_tag="VFF22_10825"
     CDS             3538..4833
                     /gene="hemA"
                     /locus_tag="VFF22_10825"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019825111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HZX17344.1"
                     /translation="MAFIALGINHKTASVDVRERVAFTPEQLVEALQQLCQRTSSREA
                     AILSTCNRSELYLEQDDLSADEVLTWLANYHRLSLEELRACAYIHADDEAVRHMMRVA
                     SGLDSMVLGEPQILGQMKSAFAVAREAGTLGPLLGRLFQATFSTAKTVRTDTAIGENP
                     VSVAFAAVSLAKQIFSDLHRSQALLIGAGETISLVARHLHDQGIKRIVVANRTLERAS
                     SLAEQFGAHAVLLSEIPDELVNSDIVISSTASQLPILGKGAVERALKQRKHKPIFMVD
                     IAVPRDIEPEVGELDDVYLYTVDDLHEVIAENLKSRQGAAQAAEELVSVGARDFMLRL
                     RELAAVDVLKAYRQQAERLRDEELNKALRLLNNGSSAEDVLALLARGLTNKLLHAPSV
                     QLKKFSADGRVDALGVAQELFALDEGVSPASTPTGLANK"
     gene            4843..5925
                     /gene="prfA"
                     /locus_tag="VFF22_10830"
     CDS             4843..5925
                     /gene="prfA"
                     /locus_tag="VFF22_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HZX17345.1"
                     /translation="MKASLINKLDLLQDRFEELTALLGDAEVISNQPQFRAYSKEYAE
                     IEPVYQAFSAFRKVQSDLEGAQALLKDSDPDMREMAEEEVAEAKEQLIELEATLQRML
                     LPKDPNDGRNVFLEVRAGTGGDEAAIFSGDLFRMYSRYAERQGWRVEVLSASEGEHGG
                     YKEVIARVEGDNVYGKLKFESGAHRVQRVPETESQGRIHTSACTVAVLPEPDEQMAID
                     INPADLRVDTYRSSGAGGQHVNTTDSAIRITHIPTGTVVECQEERSQHKNRAKAMAWL
                     AAKLKDQQEAAAHKEISDSRKLLVGSGDRSERIRTYNFPQGRVTDHRINLTLYSLNEV
                     IAGGVEAIIEPLLVEYQADQLAALGD"
     gene            6011..6844
                     /gene="prmC"
                     /locus_tag="VFF22_10835"
     CDS             6011..6844
                     /gene="prmC"
                     /locus_tag="VFF22_10835"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZX17346.1"
                     /translation="MVTIESLLNHAELPDSPTPRLDAELLLAHALGKSRSYLHTWPER
                     ELDAAQLERYQAMRVRRQAGEPVAYILGQQGFWSLELEVASHTLIPRPDTELLVETVL
                     ALLPATPAALLDLGTGTGAIALALASERPAWRLTGVDRVPEAVALAERNRARLKLANA
                     GFFASHWFSALAEQRYQLIVSNPPYIASDDRHLADGDVRFEPSSALVAGADGLDDIRL
                     IIQQAPDYLEAGGWLLLEHGFDQAAAVRELLSARGFSAVESRRDLGGHERISLGCFDN
                     E"
     gene            6864..7613
                     /locus_tag="VFF22_10840"
     CDS             6864..7613
                     /locus_tag="VFF22_10840"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HZX17347.1"
                     /translation="MLNDEELLRYSRQILLQQIDIAGQLRLKNSRALIIGLGGLGSPV
                     ALYLAAAGVGELHLADFDTVDLTNLQRQIAHDTSSIGQSKVDSASKRLLAINPQLRLQ
                     AYRQGLDSDSLAAAVAAVDLVLDCSDNFSTREAVNAACVAAKVPLVSGAAIRLEGQLS
                     VFDPRRDDSPCYHCIYGHGSEAELTCSEAGVIGPLVGLVGSLQALEALKLLAQFGEPL
                     VGRLLLIDALGSRFRELRVKRDPGCSVCGDA"
     gene            7710..8501
                     /gene="murI"
                     /locus_tag="VFF22_10845"
     CDS             7710..8501
                     /gene="murI"
                     /locus_tag="VFF22_10845"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HZX17348.1"
                     /translation="MSHPDAPVGVFDSGVGGLSVLGEIRTLLPNESLLYIADSGHVPY
                     GEKSPEFIRERCRVLAAFLLNQGAKALVLACNTATVAGVAELRELYPQLPIIGMEPAV
                     KPAAAATKAGVVGVLATTGTLKSAKFAALLDRFASDVRVITQPCPGLVERIEAGDLDS
                     AATRELLQGFVAPLLAEGCDTLILGCTHYPFLKPLLRTLVPESVSLIDTGAAVARQLQ
                     RLLDERDLLATGPARPARYWSSGDPAQMRNILSLLSGDMSVVESF"
     gene            8606..9127
                     /locus_tag="VFF22_10850"
     CDS             8606..9127
                     /locus_tag="VFF22_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HZX17349.1"
                     /translation="MNTLMRLAASAALSITGAAAGVAQAADITVAVGQSGDSTMVYRL
                     GTQFDFNRSWWQSSVGRLTGYWDAGYTFWDGDESSSNHSLSFAPVFVYEFAGEKIKPY
                     IEAGIGVAAFADTRLEGNDLGSSFQFEDRIGFGVRFAGHEVGLRAMHYSNAGLKQPND
                     GVESYTLHYRMSF"
     gene            complement(9234..9545)
                     /locus_tag="VFF22_10855"
     CDS             complement(9234..9545)
                     /locus_tag="VFF22_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008046015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZX17350.1"
                     /translation="MSQNIPHTQLADEPAVSCSNCAACCCQLEVMLITDTGVPPRYID
                     TDDWGGEVMLRLDDGWCAALDRDSMRCTIYKVRPLICREFETASPECLDERKGMSSIY
                     R"
     gene            complement(9690..10217)
                     /locus_tag="VFF22_10860"
     CDS             complement(9690..10217)
                     /locus_tag="VFF22_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 domain-containing protein"
                     /protein_id="HZX17351.1"
                     /translation="MPKLIVNTLLFQLGWFACVFGGDSLWLLIVAAVLAVHLLWTSSW
                     AAEGKLLITVFLAGSALDSFLLNLGVFDFGEPRTLIPLWLASLWLLMATTLNHCLAWT
                     AQPWWRGSLLGAIGAPLSYYGGAQIAGVGLPYGTWQSLLLIGAIWALVMPVLHGFAKL
                     YRGQYEQSQSTRKTA"
     gene            complement(10362..11627)
                     /locus_tag="VFF22_10865"
     CDS             complement(10362..11627)
                     /locus_tag="VFF22_10865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HZX17352.1"
                     /translation="MKSSSLTSAKHVVRAGSGIGASLLRRAVLRQLKQLRHGQLVIVE
                     GDERMEFGDAHSSLCAEIQVQDGAVWGLVAGNGSIGSGEAYIHGYWTTPDLTAVIRVF
                     VSNLDVLDAMESGLAQLGRPLIQSLHWLNRNTRKGSRKNISAHYDLGNDLFEQFLDPT
                     MMYSAAMFQGADDSLEQAQLNKLERICQKLALKPSDHLLEIGTGWGSMAIYAASHYGC
                     KVTTTTLSREQFAYTQQRIAERGLQGQITLLLEDYRDLAGQYDKLVSIEMIEAVGHRF
                     LPTYFEQCASLLKPDGLMLLQAITIRDQRYEQAKNAVDFIQRYIFPGGALPSVNKMLE
                     IITAKTDMNLHHMEDFGLHYARTLRIWHDNLRHARHKLEQQGYDDYFFRLWEFYLCYC
                     EGGFIERTIGTAQLLLAKPAARPAPLAAG"
     gene            complement(11614..12414)
                     /locus_tag="VFF22_10870"
     CDS             complement(11614..12414)
                     /locus_tag="VFF22_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HZX17353.1"
                     /translation="MNSALYSGWVQHRRFAPRLHNFRYRMGLLYLDLSEQEAVLALSP
                     LAGSGRFAPFAFRESDYLPQFTGAGIKLIDAVRQRVTEALGRTPLGHICLLTQPRSWG
                     LAFNPASFFYCHEADGQLAAILCEVSNTPWRERYHYVLPAEGTGHQHFAVAKAFHVSP
                     FLPRELEYRMSFSPAGERLGLHMADWSGEEKVFDASLSLQRTELNRASLHRYLLQFPW
                     MTGKTVAAIYWQALRLLLKRIPLFTHRAAEGDFRVARSASKEVPHEKL"
     gene            complement(12432..>13034)
                     /locus_tag="VFF22_10875"
     CDS             complement(12432..>13034)
                     /locus_tag="VFF22_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZX17354.1"
                     /translation="APLTESFKQHIRLNCPVTRVERDADGVTLHSAAGTERFDKVIFA
                     CHSDQALALLAEPTRIEKEILGALPYADNDVVLHTDTRLLPKRPLAWASWNYRLGGPA
                     DQPAAVTYDMNILQGIESDTTFCVSLNQTAAIDPSKILARYTYAHPQYSLAGMAAQAR
                     WEELLGANHSYFCGAYWANGFHEDGVVSALRVAREFGEAL"
BASE COUNT         2378 a         3775 c         4230 g         2651 t
ORIGIN      
        1 gctccagggc ggaaatggca ggggcggctg gattcgaacc aacgcatgcc aggatcaaaa
       61 cctggtgcct taccgcttgg cgacgcccct attaccgtac aactctgtgc tcttaattac
      121 taagagcgaa cattcacttc ccggccaagg tttcgagctt gcggtgcaac atcgagatgt
      181 tacggccttg ggcaataaaa cttggcaaag tggctggaag ttggcgggcg actttatcag
      241 catcgtcctg gtttgggaag ctcccaaaca cacaagctcc agtgccggtt aatctggctt
      301 gaacaaattt gttcaacaag atcaaagcgt tacgaacttc tggataacgc ttcgtgacca
      361 ccggctggca gtcattacga ccacccccct caagaaggct gcgaacttta atgggcggcg
      421 tatcccgtgt caactcgggg tcggagaaaa tttccgctgt gctgacaagt acttgcggaa
      481 tcgcgacaag aaaccagggt tcgcgcaggg tcaccggttg cagcttttca cctacacctt
      541 cagcaaaggc ggcgtgaccg cgcacgaata ccggcacgtc tgccccaagg ctcaagccca
      601 gggtggccag ttggtcttcg ctgcacttca gtttccacag gtagttgagg ccgagcaggg
      661 tggtagcggc atccgagcta ccgccgccaa tgccgccgcc catgggcaaa cgtttgtcca
      721 gccagatatc ggcacccaga gtgcagttcg cgtgctgttg tagcaggcgc gcggcacgca
      781 cgatcaggtt gctgtcgtgg ggtacgccgt cgatgggcgt atgcaggtga atttcaccgt
      841 cggagcgcag ggcaaagccc agctcgtcac cgaagtcgag aaactgaaac agagtttgca
      901 gctcgtgata gccgtcggcg cggcggccga gaatatgcag catcaggttg agcttggccg
      961 gtgccggcag aatcagttcg gcagcggcgg gtattgccgg ggcgctcatt gaccgagctg
     1021 gcgcggttgc cagtccttga tcaccagggt gacttgcaga tcaaagccgc tgagtttgat
     1081 gcgctcgggc agccagaagc cattctgctc ggcataactc aggtattcga catgccagcc
     1141 gtcctggctc agttgcgcca ggtggctttg gctgtcgagg ttcaggcggc ttttgctatc
     1201 gggggagggc aggccacgaa tccaccagag caggtgcgaa accggtaggt tcagcccgag
     1261 ctggtcttgc agcagctgtt ccggcgactc ggcctggtag cgcccctggt tggccacttc
     1321 cagctgaatg gcaccggggc ggccggtcag gcgagcagcg ccgcgaccca gcgggccgga
     1381 caggcggatg tcgtaatagt cttggcgttg tagccagaac agggtgccac tgccggaatc
     1441 tttcggtgcg cggatgccga ctttgccgtt gatctgccag gcgtccaggt tgctgatctg
     1501 ctgcttgtgg gcttgccagc tttgcgcatc accttggcct tcgagggctt cgcgggaggt
     1561 caggccggca cagcctgcca ataatgtgat caggctgaaa actatcaggt ggcgcgcaaa
     1621 catcagagtg tctctgctcc ggtcaggcgc agcagggtgc tgcgcaggat tgtgctgtca
     1681 ggttgctgtt tcagggcttc gcgccagagt ttgcgcgctt ctttctgctt gccttgagcc
     1741 cacagcactt cgcccagatg agctgcgact tcgtggtcgg ggaactcctg caacgcttgg
     1801 cgcagcaagc gttcagcctc gtccaggttg cccaggcggt aatttaccca gccgaggctg
     1861 tcgagtactg ccgggtcatc agggttgagc tggtgcgcct gctcgatcag ctgtttggct
     1921 tcggcgtagc gcgtggtgcg gtctgccagg gtgtagccca gggcattaag tgccatggca
     1981 ttgtccggtt cgcgctcgag tataaaacgc aggtcacgct ccagcagggc tagatcatcg
     2041 cgtttttccg cgagcatggc gcgggtgtag agcaggttga ggtcttccgg gaactgttcc
     2101 agggcttgct ggatcagctg ccaggcgcgc tcggtgttct gctgcttgga ccaggcctca
     2161 gcctcgatca ggtacagctg gatggcgtag tccggctggc tttcacgggc ctggagcagg
     2221 cgttccgagg cttcctcgcc acgctgttct ttgaacagca gatcgctctg tcgtgcctgg
     2281 gcgggcaggt agtcattgcc ggggccaacc agggcatact caatcagtgc gctgggggta
     2341 tcgcctagtt cttcataggc gcgagccagg ttgtagtgcg ccgcttcgac atggctgccg
     2401 cgttcgacca gttcttccag gtagacaatc gcctctttcc aggctttggc ttccaggcag
     2461 accagtgcca gagaaaagcg caggtcatcg tcattggggt tttgctgcag cagcgaggca
     2521 aactcgccct tggcatcgtc caggcggtct tcggccacca gcaggcgggc gtaggtcagg
     2581 cgcagacgtt tgtcatccgg gtgctgctcg ataccttttt ccagcagcgg tagggcgtca
     2641 tcgttgcgct ctaggctttg cagcaggcgc gcatgcagca ggatcgaggc gatggggcga
     2701 tctttgctgg gttgttgctc cagcagttcc agggcttctt gctgtcggcc atcctgttgc
     2761 agcaacaggg ctttgccaaa ttgcagctgg ctgttgtccg ggtatttcgc ctgcaggcgg
     2821 ttgaagcttt gcagcaggcc ggcgcgggta tccgggtcgg tttcagcggc ggacagggcg
     2881 aggaaatcga aatgcgtatc gccctgacgc tgcagcacct gctccataaa ggtcatggat
     2941 tcgtcatagc gaccagcgcg ggccaactgc acggcggcgg cgcgttgcgc gtcgatattc
     3001 tcgggcgagg ttttggccca cagcaggctg gtctccagtg cgggctgctc ggcgcctagg
     3061 tactcggcta tgcgaaaacc gcgctcggcg acgccagcat cctgggtggc gttggcctgc
     3121 tgcacatagt tttccagggc aatatcgaag cggttgcgtt ggcccgccag ttccgcaatc
     3181 agcagcgaca gcagggtttc ctggctgaag ttgcggtaaa cctgcggctg ggtttctgtg
     3241 gtgacaacag cggggtcttc caccggtggc gtgccatccg gggcagaggg ggcaaaagtt
     3301 tgacagccgc ctaataaggc agcggcagtc agtaaggcga aggatctatt cataggaaga
     3361 gtatgtggcc aacctgcggt ttgggcatca tgacacaagc gttggggcaa gcccatgggc
     3421 ggccatgatc ctggttagag agataaacct gcctattggg acaatcggca gcagtggttg
     3481 ttctcgcttt gctgaagtag gacaattgcc ggcttccact ccctgtcagc gacgtgcatg
     3541 gcctttattg cccttggtat caaccacaag accgcctcgg tggacgtccg cgaacgcgtg
     3601 gctttcactc ccgagcagtt ggttgaagca ttgcagcagc tgtgtcagcg caccagcagc
     3661 cgggaagcgg cgattctctc gacctgcaat cgcagtgagc tgtacctcga acaggatgac
     3721 ctgagcgctg atgaggtgct gacctggttg gccaactatc accggctgag cctcgaagag
     3781 ctgcgcgcct gcgcctatat ccatgccgat gacgaagccg tacggcatat gatgcgcgtg
     3841 gcctcggggc tggattcgat ggtgttgggc gagccgcaga ttctcggcca gatgaaatcg
     3901 gcctttgccg tggcccgtga ggccggcacc ctcggcccgc tgctcgggcg cttgtttcag
     3961 gccaccttca gcaccgccaa gaccgtgcgc accgataccg cgattggtga aaatccggtc
     4021 tccgtggcct ttgctgccgt cagcctggcc aagcagattt tctccgactt gcaccgcagc
     4081 caggcgttgc tgatcggtgc tggcgagacc atcagcctgg tcgcccgtca tctgcatgat
     4141 cagggtatca agcgcatcgt cgtggccaac cgcaccctgg agcgcgccag cagcttggcc
     4201 gagcagttcg gcgcccacgc cgtgctgctc tcggaaatcc ccgatgagct ggtcaacagc
     4261 gatatcgtga tcagttccac cgccagtcag ctgccgattc tgggtaaagg cgcggtagag
     4321 cgtgcgctga agcagcgcaa gcacaagccg atctttatgg tcgacatcgc cgtaccgcgc
     4381 gatatcgagc cggaagtcgg cgagctggat gacgtctatc tgtacaccgt cgatgacctg
     4441 catgaggtga tcgcggaaaa cctcaaaagc cgtcagggcg cggcccaggc tgctgaagag
     4501 ttggtcagcg tcggcgctcg cgactttatg ctgcgtttgc gtgaactggc ggcggtggat
     4561 gtgctcaagg cctaccgcca gcaggccgag cgcctgcgtg acgaggagct gaacaaggcc
     4621 ctgcggctgc ttaataatgg cagctcggcc gaggacgtgc tggcgctgtt ggctcgcggc
     4681 ctgaccaaca agttgctgca tgcgcccagt gtgcagctga aaaaattctc cgccgatggc
     4741 cgcgtcgatg cgctgggcgt ggctcaggag ctctttgctc tggacgaagg cgtgtcgccc
     4801 gcttcgaccc ctaccggctt ggccaataaa taaaggtctg caatgaaagc gtcattgatc
     4861 aacaaactcg acctgttgca ggatcgtttc gaagagctga ccgcgctgct tggcgatgcc
     4921 gaagtgatca gcaaccagcc gcagttccgt gcctattcca aggagtacgc cgagattgag
     4981 ccggtgtatc aggcgttcag tgccttccgt aaagtccagt ccgacctcga aggcgcccag
     5041 gcactgctca aagacagcga cccggatatg cgcgagatgg ccgaggagga agtcgccgag
     5101 gccaaggaac agctgatcga actggaagcc acgctgcagc gcatgctgct gccgaaagac
     5161 cctaacgatg gtcgtaacgt gttcctcgaa gtgcgcgccg gtactggcgg cgacgaggcg
     5221 gcgatcttct ccggcgacct gttccgcatg tattcgcgtt atgccgagag gcagggttgg
     5281 cgcgtcgagg tgctgtcggc cagcgaaggc gagcatggcg gctataagga agtgatagcc
     5341 cgggtcgagg gcgataacgt ctacggcaag ctcaagttcg agtccggggc gcaccgtgtg
     5401 cagcgcgtgc cggaaaccga atcccagggc cgtatccaca cctcggcttg taccgtcgcc
     5461 gtgctgcccg agccggatga gcagatggcc atcgacatca acccggccga cctgcgcgtg
     5521 gatacttacc gcagctccgg cgccggtggg cagcacgtca acaccaccga ctcggcgatc
     5581 cgcatcaccc acattcccac cgggaccgtg gtggaatgtc aggaagagcg ttcgcagcac
     5641 aagaaccgcg ccaaggccat ggcctggttg gcggccaagt tgaaggacca gcaggaagcc
     5701 gccgcgcaca aggaaatttc cgattcgcgc aagctgctgg tgggctctgg cgaccgctcc
     5761 gaacgcatcc gcacctacaa cttcccgcaa gggcgggtga ccgatcaccg tatcaacctg
     5821 accctgtatt cgctcaatga agtgattgcc ggcggcgtcg aagcgattat cgagccgctg
     5881 ctggtcgaat atcaggccga tcagctggcg gcgctgggcg attgattcgc gcttttcgta
     5941 gcccggatgc aatccgggag cgaccgttga cgcatgtccc ggattgcatc cgggctacgg
     6001 aataaatgcc atggtcacca tcgaatccct gctcaatcac gccgaactgc ccgactcgcc
     6061 caccccacgc ctggacgccg agctgttgct cgcccacgcc ctgggcaagt cgcgcagcta
     6121 tctgcacacc tggccggagc gcgagcttga tgcggcgcag cttgagcgtt accaggcgat
     6181 gcgggtgcgg cggcaggccg gcgagccggt ggcctatatc ctcggtcagc agggcttctg
     6241 gagcctggag ctggaagttg cctcgcatac cctgatccct cgtccggata ccgagctgct
     6301 ggtggagacc gttctcgcgc tgctaccggc cacgcctgcc gcgctgctcg atctgggcac
     6361 cggcaccggt gcgattgccc tggccttggc cagcgagcgc ccagcctggc ggctcaccgg
     6421 tgtggaccgc gtgccggagg cggtggccct ggccgagcgc aatcgtgcac gcctcaaact
     6481 ggccaatgcc ggcttttttg caagccactg gtttagtgcc ctggccgagc agcgttatca
     6541 gttgatcgtc agcaacccgc cctatatcgc tagcgatgat cggcacctgg ccgacggcga
     6601 tgtgcgcttc gagccgagca gcgccttggt ggcgggcgcg gatgggttgg acgatattcg
     6661 cctgatcatc cagcaggcac cggactatct tgaagcaggt ggctggctgt tgctggagca
     6721 tggcttcgac caggccgcag cggtacgcga gctgcttagc gcgcgtggtt ttagcgcggt
     6781 ggaaagccgg cgtgacctcg gcggccatga gcgcatcagt ctgggatgct tcgataatga
     6841 gtgacgccat gagcccgcac gacatgctga atgacgagga actgctgcgc tacagccggc
     6901 agatccttct gcaacagatc gatatcgccg gccagttgcg cctgaaaaac agccgggcgc
     6961 tgattatcgg cctcggcggt ttgggttcgc cggtggctct atatctggcg gctgcgggtg
     7021 tcggcgaatt gcatctggcg gatttcgata cggtcgacct gaccaacctg cagcggcaga
     7081 ttgctcacga tacttccagt atcggccaaa gcaaggtcga ttcggcaagc aaacgcctgc
     7141 tggcgatcaa cccgcagctg cgcctgcaag cctatcgcca ggggctggat agcgattccc
     7201 tggccgcagc ggtggcggca gtcgatctgg tgctggattg ctcggacaac ttcagcaccc
     7261 gtgaggcggt caacgctgcc tgcgtggccg ccaaggtgcc gctggtcagt ggcgcggcga
     7321 ttcgcctgga gggccagctg tcggtgttcg atccgcgtcg cgatgacagc ccttgctacc
     7381 actgtatcta cggccacggc agcgaagccg agctgacctg cagcgaggcc ggcgtgattg
     7441 gcccgctggt tgggttggtc ggcagtctgc aggcgctgga agcgctgaaa ctgctcgcgc
     7501 agtttggcga gccgctggtt ggccgcctgc tgttgatcga tgccctcggc agccgtttcc
     7561 gtgagctgcg ggtcaagcgt gatccgggct gcagcgtgtg tggcgatgcg tagggtggac
     7621 atggctccag ccttgtccgc cagtattgtc tgcgtggtgg acaagccttc ggcatgtcca
     7681 ccctacactt ggctgcagtg ggtaaatata tgagtcatcc agacgcaccg gttggcgtgt
     7741 tcgattccgg cgtaggcggc ctgtcggtgc tgggtgagat tcgcacgctg ttgccgaatg
     7801 agtcgctgct ttatatcgcc gacagcggcc atgtgcctta cggagaaaaa agcccggaat
     7861 ttatccgcga gcgctgccgg gtgctggccg cgtttctcct gaaccaaggg gccaaggcgc
     7921 tggtgctggc ctgtaatacc gccaccgtcg cgggggtggc cgagctgcgt gagctttatc
     7981 cgcagctgcc gattattggc atggagccag cagtaaagcc tgcggcggcg gcgaccaaag
     8041 ccggtgtagt cggtgtgctg gccaccactg gtacgctgaa aagcgccaaa ttcgccgcct
     8101 tactcgaccg ttttgccagc gatgtacggg tgattaccca gccctgccca ggtctggtgg
     8161 agcgtatcga agccggtgat ctggatagcg ccgccacccg cgagttgttg caggggtttg
     8221 tcgcgccgct gttggctgag ggttgcgata cgctgattct cggctgcacc cattacccct
     8281 ttctcaaacc gctgctgcgt accttggtgc ccgagtcggt cagcctgatt gacacgggtg
     8341 cggcggtggc gcggcagctg caacggctgc ttgatgagcg cgacctgtta gcgacgggcc
     8401 cggcgcggcc cgcgcgttat tggtcgagcg gcgatccggc gcagatgaga aatatattga
     8461 gccttttgtc gggtgatatg tcagttgtag agtcgttctg acagttctat aaccaaaatt
     8521 actgggccgt tgttgcgttc acgcgctgtt ggcctggtat gacataagta gccgagatgt
     8581 tgtctcgtca aaaaaggatg attcgatgaa cacactgatg cgtttggctg cctctgcggc
     8641 cctttccatt acgggggcgg ccgcgggtgt tgcgcaagcc gccgatataa ccgttgctgt
     8701 tggtcaatcg ggtgattcga caatggtcta tcgccttggc acgcaattcg atttcaaccg
     8761 tagctggtgg cagagcagcg ttggccgcct gacgggctac tgggatgccg gctatacctt
     8821 ttgggatggc gatgagtctt ccagcaacca tagcctgtcg tttgctccgg tattcgtcta
     8881 cgaatttgcc ggtgaaaaga tcaaacccta tatagaagcc ggtattggcg tggcagcatt
     8941 tgcggataca cgcttagaag gcaacgacct gggttcgtcc ttccaatttg aagatcgcat
     9001 cggtttcggt gtgcgcttcg ccgggcacga agtcggcctg cgcgccatgc attactccaa
     9061 cgccgggctg aagcagccaa acgacggggt tgagagttat accttgcatt accgcatgag
     9121 tttttaagat tgctgatcaa aaaaaccgga tcgtgatccg gtttttttct gcctgcattt
     9181 ttgcgccgtc tgtgccacct ggcgtggtgt aagcacccgc cagtggcatg cccttagcgg
     9241 tagatgctgc tcataccctt gcgctcgtcc aggcattcgg gggaggcggt ttcgaattcg
     9301 cggcagatca gcgggcgcac tttgtagatg gtgcagcgca tgctgtcgcg gtccagcgcg
     9361 gcgcaccagc cgtcgtccag gcgcagcatc acttcgccgc cccagtcatc ggtgtcgatg
     9421 tagcggggcg gcacaccggt atcggtgatc agcatgactt ccagctggca gcagcaggcg
     9481 gcgcagttgc tgcagctgac tgcgggttca tccgcgagtt gggtatgggg gatgttctga
     9541 ctcatgccac gcagtgtacg tcatcggtgt tattgcggcg tctgccgccg acgtagggtg
     9601 gagatggcga agccttgtcc accgatggcg gcccgaatgg cgtacaagcc ttcggcatgt
     9661 acgccctacg gtttttcagg tgctgcaacc taggctgttt tgcgagtgct ttggctctgt
     9721 tcgtactggc cgcgatagag cttggcaaaa ccatgcagca cgggcatcac cagggcccag
     9781 atcgcgccga tcagcagcaa actttgccag gtgccgtagg gcaggccgac gccggcaatc
     9841 tgcgcgccgc cgtaataaga cagtggcgca ccaatcgcgc caagcaaact gccacgccac
     9901 cagggttgtg cggtccaggc caggcagtgg ttgagcgtgg tggccatcag tagccacagg
     9961 ctggccagcc acagcggtat cagtgtgcgt ggctcgccga agtcgaagac gccgaggttg
    10021 agcagaaaac tgtccaacgc gctgccggcg agaaacactg tgatcagtag tttgccttcc
    10081 gccgcccagc tgctggtcca cagcagatgc accgccagca ccgctgcgac aatcagcagc
    10141 cacaggctgt cgccgccgaa gacgcaggcg aaccagccga gctgaaatag cagggtgttg
    10201 acgatcaatt taggcattga gatttcccag tagcaggctg ttgaaaaacg taggcgaggc
    10261 agccgagaca aggcaagaac ggccgaaaaa gcgcagttta cagctgtaaa tgagcatttt
    10321 gaggccgttc ttaacgccgt atcggcaacg caggtagttt ttcaaccggc tgcgagcgga
    10381 gcaggacgtg ccgcaggctt ggccagcagc aattgcgcgg tgccgatggt gcgctcgata
    10441 aagccgccct cgcaatagca caggtagaac tcccacaggc ggaagaagta atcgtcgtag
    10501 ccctgctgct ccagcttgtg ccgcgcgtgg cgcaggttgt cgtgccagat gcgcagggtg
    10561 cgggcatagt gcaggccgaa atcttccata tggtgcaggt tcatatcggt cttggcggtg
    10621 atgatttcca gcatcttgtt caccgagggc aacgcgccgc cggggaagat atagcgctgg
    10681 ataaagtcca cggcgttttt cgcttgctca tagcgctggt cgcggatggt gatggcctgc
    10741 agcagcatca ggccgtcggg cttgagcagg ctggcgcatt gctcgaaata ggtgggcagg
    10801 aagcgatgac cgacggcttc gatcatctcg atcgatacca gcttgtcgta ctgcccggcc
    10861 aggtcgcggt agtcctccag cagcagggtg atctgcccct gcagaccccg ctcggcaatg
    10921 cgctgctggg tgtaggcgaa ctgctcgcgc gacagggttg tggtggtcac cttgcagccg
    10981 tagtggctgg cggcgtagat cgccatgctg ccccagcccg tgccaatttc cagcaggtgg
    11041 tcgctgggtt tcagcgccag cttctggcag atgcgttcga gcttgttcag ctgcgcctgc
    11101 tccaggctgt cgtcggcacc ctggaacatc gccgccgagt acatcatggt cgggtcgaga
    11161 aactgctcga acaggtcgtt acccaggtcg taatgcgccg agatgttctt gcgtgagccc
    11221 ttacgggtgt tgcggttgag ccagtgcagg ctctggatta gtgggcggcc cagttgcgcc
    11281 aggccgctct ccatggcgtc gagcacatcc aggttgctga caaatacgcg gatcaccgcg
    11341 gtcaggtccg gggtggtcca gtagccgtgg atataggctt cgcccgagcc gatcgaaccg
    11401 ttgcccgcca ccagccccca gaccgcgcca tcctgcacct ggatctcggc gcaaaggctg
    11461 gagtgggcgt cgccgaactc catgcgttcg tcaccttcaa cgatcaccaa ctggccatga
    11521 cgcagctgtt tcagctggcg cagtaccgca cggcgcagca ggctggcacc gatgccgctg
    11581 ccggcgcgca caacatgttt ggcggaggtc aggctagagc ttttcatggg ggacttcctt
    11641 tgatgcggag cgggcgacgc ggaaatcacc ttcagcggcc cgatgggtga acagggggat
    11701 acgtttgagc agcaggcgaa gggcctgcca gtagatggcg gcaacggttt tgccggtcat
    11761 ccagggaaac tgcagcaagt agcggtgcag gctggcgcgg ttcagttcag tgcgttgcag
    11821 gctgagactg gcgtcgaaga ctttctcctc gccgctccag tcggccatat gcagccccag
    11881 gcgctcgccg gccgggctga agctcatgcg gtattccagc tcgcgcggca gaaacggtga
    11941 cacgtgaaac gccttggcca ccgcgaagtg ctgatggccg gtgccttcgg ccggcagtac
    12001 gtagtggtag cgctcacgcc aaggcgtatt gctcacctcg cagagaatcg ccgccagctg
    12061 gccatcggcc tcatggcagt agaagaaact tgccgggttg aacgccaggc cccagctgcg
    12121 tggttgggtc agcaggcaga tatgccccaa cggcgtgcgg cccagcgctt ccgtgacgcg
    12181 ctggcgtacg gcgtcgatca gcttgattcc ggcaccggtg aattgcggca ggtaatcgct
    12241 ctcgcgaaag gcaaagggcg cgaagcggcc actgccggcc agtggcgaca gcgcgagcac
    12301 ggcctcctgc tcgctcaggt cgaggtagag caggcccatg cgatagcgga agttgtgcag
    12361 gcgcggcgca aaacgccggt gctgcaccca gccgctatac agggcgctgt tcattaccgt
    12421 tgggctgttc actacagcgc ctcgccgaat tcacgggcca cgcgcagcgc gctgaccaca
    12481 ccgtcttcgt ggaagccatt ggcccagtag gcgccgcaga agtagctgtg gttggcgccg
    12541 agcagctctt cccagcgcgc ttgcgcggcc atgccggcca ggctgtattg cgggtgggcg
    12601 taggtgtagc gcgcgagaat cttgctcggg tcgatggcag cggtttggtt caggctgacg
    12661 cagaaggtgg tgtcgctctc gatcccttgc aaaatattca tgtcataagt gacggctgct
    12721 ggctgatccg ctgggccgcc caggcgatag ttccagctgg cccaggccag tggtcgtttt
    12781 ggcagcaggc gggtgtcggt gtgcagcacc acatcgttgt cggcataggg cagtgcgccg
    12841 aggatttcct tctcgatgcg tgtaggttct gccagcaggg ccagggcctg gtcgctgtgg
    12901 caggcgaaga tcaccttgtc gaagcgttcg gttccggcgg cgctgtgcag ggtcacaccg
    12961 tcggcatcac gctcgacgcg ggttaccggg cagttgaggc ggatatgctg cttgaaggac
    13021 tcggtcagcg gcgc
//
LOCUS       DASRUM010000166        10262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1430321, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000166 DASRUM010000000
VERSION     DASRUM010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 10262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10262
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430321"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFF22_10880"
     CDS             complement(<1..180)
                     /locus_tag="VFF22_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZX17355.1"
                     /translation="MRIISVNVNGIHAAVERGLLSWLQAQNADVICLQDTRASAFELD
                     DQALQLDGYFLYACDA"
     gene            274..915
                     /gene="pyrE"
                     /locus_tag="VFF22_10885"
     CDS             274..915
                     /gene="pyrE"
                     /locus_tag="VFF22_10885"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HZX17356.1"
                     /translation="MQAYQRDFIRFAIERGVLRFGQFTLKSGRISPYFFNAGLFDSGL
                     ALSQLGRFYAAAVVDSGIDFDVLFGPAYKGIPLAATTAVALAEHHQRDMPWCFNRKEA
                     KDHGEGGTLVGAPLAGRVLIIDDVITAGTAIREVMQIIQAQGAQAAGVLIALNRQERG
                     KGELSAIQEVERDYGMPVVSIVSLEQVLEYLAEDAELKQYLPAVEAYRAEYGI"
     gene            939..1532
                     /locus_tag="VFF22_10890"
     CDS             939..1532
                     /locus_tag="VFF22_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HZX17357.1"
                     /translation="MYRPVLTRCSLLLGLLLPVVAGAADLYRYVDDKGTTVLSRQGVP
                     PEHISKGYEVLNDQGRVIQVIPPAPSPEEMKRILAEKARASSDAQLLRLYSTPEDVER
                     ARERKLAELDGLIGVARGNLQSVRTQQANLQSQAADHERAGRAVPAHLLAQIDNQKAE
                     QARLKGDILRYQAARKEADSSFNADRDRLKELLGRNQ"
     gene            complement(1535..1981)
                     /locus_tag="VFF22_10895"
     CDS             complement(1535..1981)
                     /locus_tag="VFF22_10895"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZX17358.1"
                     /translation="MPTAPSRDAFSFFHTLRVRWAEVDPQSIVFNGHYLTYADVAITE
                     YFRALDVRYPEDLSRDGGDFFAIKTVLEYLAPARFDDELQIGIRVARLGRSSLSFAMG
                     IWCGEQALTTGEVIYVHADSASRSSSPLPAWLREKVLAFERCAPQQ"
     gene            complement(2116..3021)
                     /gene="argB"
                     /locus_tag="VFF22_10900"
     CDS             complement(2116..3021)
                     /gene="argB"
                     /locus_tag="VFF22_10900"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZX17359.1"
                     /translation="MTLERDAATNVAKVLSEALPYIRRFVGKTLVIKYGGNAMESEEL
                     KQGFARDIVLMKAVGINPVVVHGGGPQIGDLLKRLSIESHFVDGMRVTDTATMDVVEM
                     VLGGQVNKSIVNLINQHGGSAIGLTGKDAQLIRAKKLTVTRQTPEMTQPEIIDIGHVG
                     EVTGVNIDLLNMLVKGDFIPVIAPIGVGPDGESYNINADLVAGKVAEALKAEKLMLLT
                     NIAGLMDKQGQVLTGLTTEQVDGLIADGTIYGGMLPKIRCALEAVQGGVTSAHIIDGR
                     VPNAVLLEIFTDSGVGTLICNRKRH"
     gene            complement(3053..4441)
                     /gene="algC"
                     /locus_tag="VFF22_10905"
     CDS             complement(3053..4441)
                     /gene="algC"
                     /locus_tag="VFF22_10905"
                     /EC_number="5.4.2.2"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HZX17360.1"
                     /translation="MQAKPPRLPADIFRAYDIRGVVGRTLNAETAYWIGRAIGSQSLA
                     QGEPNIAVGRDGRLSGPELAQQLIQGLLDCGCHVSDVGMVPTPVVYYAGHILTGKSAV
                     MLTGSHNPADYNGFKIVIAGDTLANEQIQALKARIDNNDLASGVGTVEQVDVLERYFK
                     QIRDDIAMAKPMRVVVDCGNGVAGVIAPQLIEALGCSVIPLYCEVDGNFPNHHPDPGK
                     PENLVDLIAKVKSEKADIGLAFDGDGDRVGVVTNTGAVIYPDRLLMLFAKDVVSRNPG
                     ADIIFDVKCTRRLTPLISGYGGRPVMWKTGHSLIKKKMKETGALLAGEMSGHIFFKER
                     WFGFDDGIYAAARLLEILSQDRRDADHVFSAFPNDISTPEINIQVTEQSKFSIIERLQ
                     RDGVWGEGNITSLDGVRVDYPKGWGLVRASNTTPVLVLRFEAETEQELERIKEVFRAQ
                     LYSAVPDLDLPF"
     gene            complement(5698..6153)
                     /gene="dut"
                     /locus_tag="VFF22_10910"
     CDS             complement(5698..6153)
                     /gene="dut"
                     /locus_tag="VFF22_10910"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HZX17361.1"
                     /translation="MHALQAKILDPRLGNEFPLPHYATPGSAGLDLRAMLKQELVLEP
                     GQTVLIPTGLSIYIGDPGLAAMILPRSGLGHKHGIVLGNLVGLIDSDYQGELMVSCWN
                     RGQTPFTIAIGERIAQLILVPVVQAHFELVEQFDESQRGAGGFGHSGSH"
     gene            complement(6182..7390)
                     /gene="coaBC"
                     /locus_tag="VFF22_10915"
     CDS             complement(6182..7390)
                     /gene="coaBC"
                     /locus_tag="VFF22_10915"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZX17362.1"
                     /translation="MQRLYRKRIIVGVGGGIAAYKSAELIRRLKDQDADVRVVMTKGG
                     REFITPLTLQALSGHPVHLDLLDPEAEAAMGHIELARWADLVLIAPATADLMARLAQG
                     VADDLLTTLVLATDAAVALAPAMNQAMWRDPATQANAALLTARGLHLFGPAAGSQACG
                     DVGLGRMLEAEQLVQCAADCFQYQALTGKHVLITAGPTQENIDPVRYITNHSSGKMGF
                     ALAEAAAEAGAKVTLISGPVHLPTPERVTRINVTSARDMLAACEAAMPCDLLIAAAAV
                     ADYRPEVVAQHKLKKDPTSGDGLLLQMVRNPDILATLAGRSDRPFSVGFAAETENLLE
                     YASRKLRDKNLDLIVANDVANPSIGFNSEENAITIIDRELQQSSFAQTSKSKIARQLV
                     SFIADRLDRS"
     gene            7581..8255
                     /gene="radC"
                     /locus_tag="VFF22_10920"
     CDS             7581..8255
                     /gene="radC"
                     /locus_tag="VFF22_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HZX17363.1"
                     /translation="MSIRDWPAAERPREKLLAQGAGTLTDAELLAIFLRTGVAGQSAV
                     DLARHLLGDFGSLRALLQADLPLFSQRLGLGPAKFAQLQAVLEMARRHLAEQLRRDSA
                     LESPQAVRDYLKALLRHEPHEVFGCLFLDAKHRVLAFEALFHGSIDSASVYPRQVVKR
                     ALAHNAAALILTHNHPSGVAEPSQADRVLTRRLKEALELVDVRVLDHFIVGDGEPLSM
                     AEYGWL"
     gene            complement(8252..9841)
                     /locus_tag="VFF22_10925"
     CDS             complement(8252..9841)
                     /locus_tag="VFF22_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016975922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX17364.1"
                     /translation="MRLAALPLLLAPLLAPALVQANTLSVCTEASPDGFDVVQYNSLT
                     TTNASADVLMNRLVDFDATSGTLQPSLAQAWEVSPDGLSYSFTLRPDVQFHTTEYFSP
                     SRALNADDVLFSFQRMLDPAHPWHKVAPSGYPHAQSMQLPSLIKSIDKTGEHSLRFSL
                     NRPDATFLATLSMGFASIYSAEYAAQLLAAGTPEALNSQPIGSGPFVFKRFQKDASVR
                     YSTNLSYFGGKPAVDNLVFAITPDANVRLQKLRRGECHIALSPKPQDVQSISGDNNLQ
                     SAHTAAFMTAFVGINSQHPPLDQPAVRQAINLAFDKASYLKAVFENSAEAANGPYPPN
                     TWSYASELPGYPHDPQKARELLKQAGLADGFTTTIWTRPTGSVLNPNPSLGAQLLQAD
                     LAKVGIKAEIRVIEWGELIRRAKAGEHDLLFMGWAGDNGDPDNFLTPQFACASLESGL
                     NFARYCDPTLDKLIADGKSTNDQTQRSQLYKRAQTIIQQQALWLPLAHPTAYALTRKQ
                     VEGYQVSPFGRQDFSKVQLVP"
     gene            10191..>10262
                     /locus_tag="VFF22_10930"
     CDS             10191..>10262
                     /locus_tag="VFF22_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L28 family ribosomal protein"
                     /protein_id="HZX17365.1"
                     /translation="MSRVCQVTGKGPVTGNNISHANNK"
BASE COUNT         1785 a         2882 c         3468 g         2127 t
ORIGIN      
        1 tgcatcgcag gcatagagga aatagccatc cagttggagg gcttggtcgt ccagttcaaa
       61 ggcggaggcg cgggtgtcct gcaggcagat gacgtcggca ttctgtgctt gcagccaact
      121 gagcaaaccg cgctcgactg cagcatgaat accattcacg ttcacactga tgatccgcat
      181 aaatggcccc caaaatcacg tgcgtgtatg atacccgagc tcacctctat tagctaaatt
      241 acctagtaaa tccgtgctgt ccggggcttt ttcatgcaag cgtatcaacg tgatttcatc
      301 cgttttgcca tcgagcgtgg ggttctgcgc tttggtcagt tcacccttaa gtcagggcgc
      361 atcagtcctt atttcttcaa tgccgggctg tttgacagcg gtttggccct gtcccagctc
      421 gggcgtttct acgcggcggc tgtggtggac agcggtatcg actttgacgt gctgtttggc
      481 ccggcctaca agggtattcc gctggctgcg accaccgcag tggcgctggc tgagcaccat
      541 cagcgtgata tgccctggtg tttcaaccgc aaggaagcca aggatcacgg cgaaggtggc
      601 acgctggtgg gcgcgccgct ggccggacgc gtgttgatca ttgatgacgt gatcactgca
      661 ggcacggcga ttcgtgaagt gatgcagatc atccaggcgc agggcgcgca ggcggccggt
      721 gtgctgatcg cgttgaaccg ccaggagcgc ggtaaaggtg agctgtcggc gattcaggag
      781 gtcgagcgcg actatggcat gccggtggtg agcattgtgt cactcgagca ggtactggaa
      841 tatctggcag aggatgctga actcaagcag tatctgccag cggtagaagc ctaccgtgcc
      901 gaatacggaa tctaacctgc cgataaggtg ctccattgat gtatagacct gtcctgaccc
      961 gctgttcgct gttgctaggc ctactgctgc cggttgtggc gggcgcggct gatttgtacc
     1021 gctacgtcga tgacaaaggc accacggtgc tcagccgtca gggtgtgccg ccggagcata
     1081 tcagcaaggg ttatgaagtg ctgaatgacc aggggcgggt gatccaggtg attccaccgg
     1141 cgcccagccc tgaggagatg aagcgcatcc ttgctgaaaa ggcccgcgcc agctccgatg
     1201 cgcagctgct gcgcctatac agcacgccgg aagatgtgga gcgcgcgcgt gagcgcaagc
     1261 tggccgagct ggatgggttg attggtgtgg cacgcggcaa cctgcagtcg gtgcgcaccc
     1321 agcaggccaa tctgcagagc caggctgctg accatgagcg tgccgggcgt gcggtgccgg
     1381 ctcatctgtt ggcgcagatc gataaccaga aagccgaaca ggcgcgtctc aagggcgaca
     1441 tcctgcgcta tcaggcggcg cgcaaggaag ccgacagtag cttcaacgct gaccgtgatc
     1501 ggctcaagga actgctgggc cgcaaccagt aaggctattg ctgcggtgcg cagcgttcaa
     1561 aggccagaac tttctctctc aaccacgccg gtagtggact gctgctgcgg cttgcgctgt
     1621 ccgcgtgtac gtaaatcact tcgccggtgg tcagcgcctg ttcgccgcac cagatgccca
     1681 tggcaaagct caggctcgag cggcccaggc gggcgacgcg aatgccgatc tgtagttcat
     1741 catcgaagcg tgccggtgcc aggtactcca gcacggtctt gatcgcgaag aagtcgccgc
     1801 cgtcgcggct caggtcttcc gggtagcgaa cgtccagcgc gcggaagtat tcggtgatgg
     1861 cgacatcggc gtaggtcagg tagtgaccgt tgaacacgat gctctgcgga tcgacctcgg
     1921 cccagcgcac gcgcagggtg tggaaaaagc tgaaggcatc gcgggaaggc gctgttggca
     1981 tgcggtacac ctctggctgg cagtgggaat cactgagctt cggtgctgct gcggctcgct
     2041 gcaagggttg cagcaggcta tcaggcggct gaatcagcca gaattacccg gctgattcgc
     2101 attgcaggca ggagcttagt gacgcttgcg gttgcagatc aatgtgccta caccgctgtc
     2161 ggtgaaaatt tccagcagta cggcattcgg cacgcgaccg tcgatgatat gcgcgctggt
     2221 cacgccgcct tgcaccgctt cgagggcgca gcggatcttc ggtagcatgc cgccgtagat
     2281 ggtgccgtcg gcgatcaggc cgtcgacttg ctcggtggtc aggcctgtaa gcacctggcc
     2341 ttgtttgtcc atcagcccgg cgatattggt caacagcatc agcttttcag ctttcagcgc
     2401 ttcggcgacc ttgccggcga ccaggtcggc gttgatgttg tacgactcgc cgtccgggcc
     2461 gacgccaata ggtgcgatca ccgggataaa gtcgcccttg accagcatat tcagcaggtc
     2521 gatgttgact ccggtgactt cgccgacatg gccgatatcg atgatttccg gttgggtcat
     2581 ctccggcgtc tggcgggtca cggtcagttt tttggcgcgg atcagctggg cgtctttacc
     2641 ggtcaggccg atggcgctgc cgccgtgctg gttgatcagg ttgacgatgc tcttgttgac
     2701 ctggccgccg aggaccattt ccaccacgtc catggtcgcg gtgtcagtga cgcgcatgcc
     2761 gtcgacaaaa tggctttcga tagacaggcg cttgagcaga tcaccgatct gcggaccgcc
     2821 gccgtggacg actaccggat tgatgccgac cgccttcatc agcacgatgt cgcgggcaaa
     2881 gccttgcttg agttcttcgc tttccatggc gttgccgccg tatttgatta ccagcgtctt
     2941 gccgacgaag cggcggatat acggcagtgc ttcggacagt accttggcga cgttggtggc
     3001 ggcatcacgt tcgagggtca tgcagggctc cagtagaaaa tactctaatc aatcagaacg
     3061 gcaggtcgag gtcgggcacc gcgctataga gctgggcgcg gaagacttcc ttgatccgtt
     3121 cgagttcctg ttcggtttcc gcctcgaagc gcagcaccag caccggtgtg gtgttggagg
     3181 cgcgcaccag gccccagcct ttggggtagt cgacgcgtac gccgtcgagg ctggtgatat
     3241 tgccttcgcc ccatacgccg tcgcgttgca ggcgttcgat gatgctgaac ttgctctgct
     3301 cggtgacctg gatattgatt tccggggtgg agatgtcgtt ggggaaggcg ctgaacacgt
     3361 ggtcggcgtc gcgacgatcc tggctgagga tttccagcaa gcgcgcggcg gcatagatgc
     3421 cgtcgtcgaa gccgaaccag cgctccttga agaagatatg cccgctcatt tcgcccgcta
     3481 acagcgcgcc ggtttctttc atcttcttct tgatcagcga atggccggtt ttccacatca
     3541 ccggtcggcc gccgtagccg ctgatcagcg gggtcaggcg gcgcgtgcat ttgacgtcga
     3601 agatgatgtc ggcgccgggg ttgcgtgaga ccacgtcctt ggcgaacagc atcagcaggc
     3661 ggtccgggta gatgacggcg ccggtattgg tcaccacgcc gacgcggtcg ccgtcgccgt
     3721 cgaaggccag gccgatatcg gctttctcgc tcttcacctt ggcgatcagg tccaccaggt
     3781 tttccggctt gccggggtcc gggtggtggt tggggaagtt gccgtcgact tcgcagtaca
     3841 gcggaatcac cgagcagccc agggcctcga tcagctgcgg ggcgatcacc ccggccacgc
     3901 cattgccgca gtcgaccacc acgcgcatgg gtttggccat ggcgatgtca tcgcggatct
     3961 gcttgaagta acgctccagc acatcgacct gctcaaccgt gccgacgccg ctggccaggt
     4021 cgttgttgtc gatgcgcgct ttcagtgcct ggatctgctc gttggccagg gtgtcgccgg
     4081 cgatgacgat cttgaagccg ttgtagtccg ctgggttatg gctgccggtg agcatcaccg
     4141 ccgatttgcc ggtgaggata tgcccggcgt aatacaccac gggcgtcggc accatgccga
     4201 catcgctgac atgacagccg caatcgagca ggccctggat cagctgctgc gccagctccg
     4261 ggccggacag acgaccgtca cggccgacgg cgatgttcgg ctcgccctga gccaggctct
     4321 gcgagccaat ggcgcggcca atccagtagg cggtttcggc gttcagggtg cggccgacta
     4381 cgccacgaat gtcgtaggcg cggaagatat ccgccggcag cctgggcggt ttcgcctgca
     4441 cggggacggg tgctggctca tcgaggccga gcaggtcctg gtcttcgtca agaatatcga
     4501 tatcgagaat atcggtgtcc tggaatagcg gatcaaccat agcgctggtg ggcttttgca
     4561 tggcggcgag tcctgggttg gttgtacctg tgccggcggc tagcgcggca gctggttcag
     4621 tcgcgcggcg cggcatgcgt gcgaggttct gcgcgaggat atccaaggcc ggcagagtca
     4681 agctgaaggc tttgacggcc ttgccggcgg acagttcctt gagcatttgc cccagctgca
     4741 gcacgtcggc gcgcagatgg cgttgcaggc tgctctgcac cagatagagg ccggtaatgg
     4801 cgccagccag ggccagcagc ccggcgatgg ccagcagcag cggcgagaac accgagttgg
     4861 tcagggacgg acctggggtg aagctgatcg tccagttggg gttgccggtg ctgaatgcct
     4921 gcgcggtgcc ttcagcctgg ccgcgttggg caagcacttg ggctgcggtg ttgttgaact
     4981 gctggatcag ctggatctgg ccgatttcgg cgggcatcac tggaaggctg gagagcaggc
     5041 gctcaaggtc cacggctagc agcagagtgc cgtgcagcgg ttcgctttcg ctcaggcgca
     5101 gcggtgcggc gctgtagacc agccagcgtt ggcccacctt gtaagcctcg ggcgcaggcg
     5161 tctggccgtt ttctgcgcgg cgcagcatgt ccagcgcggc aaagttcatc ggcgcggcgc
     5221 ggcccatgtc ctgtacagcc tgggcacgag cgttgaggtg cacgtcgacc acggcatgcc
     5281 agtagctgag gttgcgctcg gcggcgcgaa tctgattgat gtcctggctt tgtagggcct
     5341 gcagcagttg cgggttgcgc gcggcggcct gggtgtcggc gctcagttgt tgcagggctt
     5401 tctgcagcac ggtcgcctga ccgccgcccc aggcttggct gagttgctgc aactgctgtt
     5461 ggtgggcgct gttgagcggg ccgaaccaga gcagtgcgcc ggccagaatg atgccgacaa
     5521 cagctgccag taccccgggc aacagggtgt tgaggctgtt cttggccgct ttgggttcgg
     5581 cgggggcact ggtcatctgg gcgctggggg ttaccccgtc agcgtccttg gcagtgcgct
     5641 tgaagagttt catgcagcgc ctcctcggca attcatcctg gaattcagtt acggcgatca
     5701 atggctaccg gagtggccga agccgcctgc gccgcgctga ctttcatcga actgttcaac
     5761 cagttcaaaa tgcgcctgca ccaccggtac cagaatcagt tgcgcgatac gttcaccgat
     5821 ggcgatggtg aatggcgtct gcccacggtt ccagcaggac accatcaatt cgccttggta
     5881 gtccgaatcg atcaggccaa ctagattgcc gaggacgatg ccatgtttgt ggcccaggcc
     5941 tgagcgcggc aggatcatcg cggccaggcc ggggtcgcca atgtagatcg acaggccggt
     6001 ggggatcagt acagtttgcc ccggctccaa caccaattcc tgcttgagca tggcgcgcag
     6061 gtcaaggccg gcggagccag gcgtggcgta atgcggcagg gggaattcgt tgcccaggcg
     6121 cgggtcgagg atcttggctt gtaaggcgtg catatcaggc tctgtagtcg gtgagtgggc
     6181 gtcagctacg gtccaggcgg tctgcaataa agctcaccaa ctggcgggca atcttgcttt
     6241 tgctggtctg ggcgaaactg ctctgttgga gctcacggtc aatgatggtg atggcgtttt
     6301 cttcgctgtt gaaaccgatg ctggggttgg ccacgtcatt ggcgacgatc agatcgaggt
     6361 ttttgtcgcg cagcttgcgc gaggcgtact ccagcaggtt ctcggtctcg gcggcaaaac
     6421 cgacgctgaa cggccggtcg ctgcgccctg ccagggtggc gagaatatcc gggttacgca
     6481 ccatttgcag gagcagacca tcaccactgg tagggtcttt cttcaatttg tgctgggcga
     6541 ccacttccgg gcggtaatcg gcgactgctg cggcggcgat cagcaggtcg cagggcatcg
     6601 cagcttcgca ggcggcgagc atatcgcggg cgctggtgac gttgatccgg gtaacgcgct
     6661 cgggcgtggg caagtgtacc gggccgctga tcagggtgac cttggcgcct gcctcggcgg
     6721 cggcttcggc tagggcaaag cccattttgc cggagctgtg gttggtgata tagcgcaccg
     6781 ggtcgatgtt ttcctgggtc gggccggcgg taatcagtac atgtttgccg gtcagcgcct
     6841 ggtactggaa gcagtcggcg gcgcactgca ccagctgttc tgcttcgagc atgcggccca
     6901 ggccgacatc gccgcaggcc tgactgccgg cggcggggcc aaacaaatgc aggccgcgcg
     6961 cagtcagcag tgcagcgttg gcctgggtgg ccgggtcgcg ccacattgcc tggttcattg
     7021 ccggggcgag agcgacagcg gcgtcggtgg ccagcaccaa ggtggtcagc aggtcatcgg
     7081 ctacgccctg ggccaggcgc gccatcaggt cggcggtggc cggggcaatc agtaccagat
     7141 cggcccagcg cgccagttcg atatggccca tggccgcttc ggcttcgggg tcgagcagat
     7201 cgaggtgcac cggatgcccg gatagggctt gcagggtcag cggggtgatg aattcacgac
     7261 cacctttggt catgaccacg cgcacgtcgg cgtcctggtc cttgagtcgg cgaatcagct
     7321 cggcactttt atacgcggca ataccgccgc caacgccaac aatgatgcgt ttccgataca
     7381 gccgctgcat aggcctgcct tttatcaaag agggttacac cgccacgccg acacctcccc
     7441 aggccaggcg cgacgcaaaa gttgggctac gatagcacag agcctgcagc ggagtagcgc
     7501 tgccaggcgc ttgccaggct tggctttgcg gcattcgggc gaggcggttt gccgcgcctg
     7561 gtaacacaca aggaggtgtg atgagtattc gtgattggcc tgcggcagag cgtccgcggg
     7621 aaaagctgct ggcgcagggg gcaggcactc tgactgatgc agagttgctg gcgattttcc
     7681 tgcgtacagg cgtagccggg cagagtgcgg tcgacctggc gcgtcattta ttgggcgact
     7741 tcggcagtct gcgcgccttg ctgcaggcgg atttaccgtt gttcagccag cgcctgggtc
     7801 tgggtccggc caaatttgcc cagttacagg cggtgctgga gatggcgcgg cggcatctgg
     7861 ccgagcaatt acgtcgcgat tcggcgctgg aaagcccgca ggccgtgcgt gattacctga
     7921 aagcgctgct acgccatgag ccgcatgagg tgtttggctg cctgtttctc gatgccaaac
     7981 accgtgtgct ggcgttcgag gcgctttttc atggttccat cgacagtgcc agcgtctatc
     8041 cccggcaggt ggtcaaacgc gcgttggcgc ataacgcagc ggcgctaatt ttgacccata
     8101 accatccatc aggcgtcgcc gaacccagcc aggccgatcg ggtgctgact cgtcggctga
     8161 aagaggcgct ggagttggtg gatgtgcgcg tactcgacca ctttattgtc ggcgacggtg
     8221 agccgctgtc gatggccgag tacggttggc tctaaggcac cagttgcact ttggaaaagt
     8281 cctggcgacc gaacgggctg acctgataac cctcgacctg cttgcgcgtc agagcgtagg
     8341 cggttgggtg ggccagtggt agccagagtg cctgctgctg gatgatcgtt tgcgcccttt
     8401 tatacagctg gctgcgctgc gtctggtcgt tggtgctttt gccatctgcg atcagcttgt
     8461 ccagcgttgg gtcgcaatag cgggcaaagt tcagcccgga ttcgagcgaa gcgcaggcga
     8521 attgcggggt gaggaagtta tccgggtcgc cgttgtcgcc ggcccagccc ataaacagca
     8581 gatcatgctc accggctttg gcgcggcgaa tcagctcgcc ccattcgatc acacggattt
     8641 ccgctttgat gccgactttg gcgaggtcag cctgtagcaa ctgtgcgccc aggctcgggt
     8701 tggggttgag tacgctgccg gtaggacggg tccagatggt ggtggtgaaa ccatcggcca
     8761 ggccggcctg cttgagcaac tcgcgggctt tttgcgggtc atgcgggtag ccgggcagtt
     8821 cgctggcgta gctccaggta ttcggcggat aggggccgtt agcggcttcg gcgctgtttt
     8881 cgaacactgc cttgaggtag ctggccttgt cgaaggccag gttgatcgcc tggcgcacgg
     8941 cgggttggtc cagtggcggg tgctggctgt tgatgccgac gaaagcggtc ataaaggcag
     9001 cggtatgcgc gctctgcaga ttgttatcac cgctgatgga ctgcacatcc tgcggtttcg
     9061 gcgacagagc gatatggcac tcgccgcggc gcagcttctg caggcgcacg ttggcgtccg
     9121 gggtaatggc gaacaccagg ttgtccaccg caggcttgcc cccgaagtag ctcaggttgg
     9181 tgctgtaacg cacgctggcg tctttctgga agcgcttgaa gacaaacggc ccgctgccta
     9241 tcggctggct gttgagcgct tccggagtgc cggccgccag tagttgcgcg gcatattcgg
     9301 cggaataaat agaggcgaag cccatgctca gggtggcgag gaaggtcgca tccgggcggt
     9361 taaggctaaa gcgcaggctg tgctcgccgg tcttgtcgat gcttttgatc aggctcggca
     9421 gctgcatcga ctgcgcgtgt ggataaccac tgggggcgac cttgtgccag ggatgggctg
     9481 ggtcgagcat gcgctgaaag ctgaacagca cgtcatcggc attcagcgcg cggctggggc
     9541 tgaagtactc ggtggtatgg aattgcacat ccggacgcaa ggtgaagctg tagctcaggc
     9601 cgtccggact gacctcccag gcctgcgcca ggctcggctg cagggtgccg ctggtcgcgt
     9661 cgaaatccac caggcggttc atcagcacgt cggctgaggc gttggtggtg gtcagtgagt
     9721 tgtactgcac cacgtcgaag ccgtctgggc tggcttcagt gcagacgctg agcgtattgg
     9781 cctggaccag ggcgggcgca agaagcgggg cgagcagcag ggggagggcg gcgagacgca
     9841 tggcaaactc ctgagtagcg gtgggcgcgc tggcgtaagg tcttacccta ggccattaac
     9901 tggcggcagg gcaatcagtc ttggcgacga gcggtcgatc tgcgctgttg ccgttgcggg
     9961 ctggcctggg cgctggagtt gcgggttggt ctttgccggg taaagtgcgt ttaaccttgt
    10021 tgcactgggg gctttctggt ataaagcagc gctcttttgt aggggcccgg ttcttgcgct
    10081 ttcaggtacg gccggtaaga ccctcgagat acgcggcgtt aagcgccaag attgaataat
    10141 tagcggccaa cccatgccgg gttgggcatg tggttttaga gggctgaggc atgtcgagag
    10201 tctgtcaagt taccggtaag ggtccggtaa ccgggaataa catttcccac gcaaacaaca
    10261 aa
//
LOCUS       DASRUM010000167         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1603911, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000167 DASRUM010000000
VERSION     DASRUM010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603911"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..2326
                     /gene="clpA"
                     /locus_tag="VFF22_10935"
     CDS             56..2326
                     /gene="clpA"
                     /locus_tag="VFF22_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HZX17366.1"
                     /translation="MLNRELEVTLNLAFKEARTKRHEFMTVEHLLLALLDNEAAASVL
                     RACGANLDKLRHDLQEFIDSTTPLIPQHDEERETQPTLGFQRVLQRAVFHVQSSGKRE
                     VTGANVLVAIFSEQESQAVFLLKQQSVARIDVVNFIAHGISKVPGHGGHSESEQEMQD
                     DEGGEPASSSNPLDAYASNLNELARQGRIDPLVGRESEVERVAQILARRRKNNPLLVG
                     EAGVGKTAIAEGLAKRIVDNQVPDLLADSVVYSLDLGALLAGTKYRGDFEKRLKALLN
                     ELRKRPHAILFIDEIHTIIGAGAASGGVMDASNLLKPMLSSGEIRCIGSTTFQEFRGI
                     FEKDRALARRFQKVDVVEPSVEDTIGILRGLKGRFEQHHSIEYSDEALRAAAELASRY
                     INDRHMPDKAIDVIDEAGAYQRLQPVEKRLTRIDVAQVEDIVAKIARIPPKHVSSSDK
                     ELLRNLERDLKLTVFGQDAAIDSLSTAIKLSRAGLKAPDKPVGSFLFAGPTGVGKTEA
                     ARQLAKALGVELIRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEAITKTPHSVLL
                     LDEIEKAHPEVFNLLLQVMDHGTLTDNNGRKADFRNVIVIMTTNAGAETAARASIGFT
                     HQDHSSDAMEVIKKSFTPEFRNRLDTIIQFGRLSHEVIKSIVDKFLTELQAQLEDKRV
                     TLEVSDAARNWLAESGYDVQMGARPMARLIQDKIKRPLAEEILFGELAEHGGVVHIDI
                     EGGELTFDFETTAEMA"
     gene            complement(2425..>2623)
                     /gene="infA"
                     /locus_tag="VFF22_10940"
     CDS             complement(2425..>2623)
                     /gene="infA"
                     /locus_tag="VFF22_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011217896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZX17367.1"
                     /translation="EMEGTVVDTLPNTMFRVELENGHVVTAHISGKMRKNYIRILTGD
                     KVRVELTPYDLSKGRITYRAR"
BASE COUNT          583 a          714 c          771 g          555 t
ORIGIN      
        1 tctgtgaaat agagaaggac ggttaacgcc ggccacttgg gcatgaggtg aagctatgtt
       61 aaatcgagag ctcgaagtca ccctcaatct ggctttcaag gaggctcgta ccaagcgtca
      121 tgaatttatg acggttgagc atttgttgct ggccctcctg gataacgagg ccgccgcgag
      181 cgtactgcgt gcgtgcgggg ccaacctgga caagctgcgg catgatctgc aggagtttat
      241 cgactccacc acgccgctga ttccccaaca cgatgaagag cgcgagacgc agcctacgct
      301 gggtttccag cgtgttctgc agcgcgcggt gttccacgtg cagagttccg gcaagcgtga
      361 agtgactggc gccaacgtgc ttgtggcgat tttcagcgag caggaaagcc aagctgtgtt
      421 tctgctcaaa cagcagagcg tcgcgcgtat cgatgtggtc aactttattg cccacggtat
      481 ctccaaggtg ccgggccatg gcggccattc ggaatccgaa caagaaatgc aggacgacga
      541 gggcggcgag cccgccagct ccagcaatcc tctggacgct tatgccagta atctgaacga
      601 actggctcgc cagggccgta ttgatccgtt ggtcggccgg gagtctgaag tcgagcgcgt
      661 ggcgcagatt ctggcccgtc gacgcaagaa taatccgcta ttagtgggcg aggcgggtgt
      721 cggcaaaacc gccatcgccg aaggcttggc caaacgtatt gtcgataatc aggtgcctga
      781 cctgctggct gacagcgtgg tctactcact ggatctcggc gcactgctgg ccggtaccaa
      841 ataccgcggc gattttgaga agcgtctgaa agccttgctc aatgagctgc gtaagcgtcc
      901 acatgccatt ctgtttatcg atgaaatcca caccatcatc ggtgccggtg ctgcgtcggg
      961 cggagtaatg gatgcgtcca acctgctcaa acccatgctg tcctcgggtg aaattcgctg
     1021 catcggttca acgaccttcc aggaatttcg cggcatcttt gagaaagacc gtgccctcgc
     1081 gcggcgtttc cagaaagtcg atgtggtcga gccctcggtg gaagacacca tcggcatcct
     1141 gcgtggcctg aaaggccgtt tcgaacaaca ccacagcatc gaatacagcg acgaagccct
     1201 gcgcgccgct gctgagctgg cctcacgcta tatcaatgac cggcatatgc cggacaaggc
     1261 cattgatgta atcgatgaag cgggggccta ccagcgcctg cagccggtgg aaaaacgcct
     1321 gacccgtatc gacgtggcgc aggtagaaga tatcgtggcg aaaattgccc gtattccgcc
     1381 aaagcatgtc agcagttcgg acaaagagtt gctgcgcaac ctcgagcgtg acctcaaact
     1441 caccgtgttt ggccaagacg ctgcgattga ctcattgtcg accgcaatca agctgtcacg
     1501 tgccgggctt aaggcgcctg acaagccggt cggctccttc ctgttcgccg gccctaccgg
     1561 ggttggtaaa acagaagccg cccgtcagtt ggccaaagcc ttgggggttg agctgattcg
     1621 cttcgatatg tccgagtaca tggagcgcca taccgtctcg cggctgatcg gtgcgccgcc
     1681 aggttatgtt ggctttgatc agggcggtct gctgactgag gcaattacta aaacaccaca
     1741 cagcgtgctg ctgctcgatg agatcgaaaa agcccaccct gaagtcttta acctgcttct
     1801 gcaggtcatg gaccacggca ccttgaccga taacaacggc cgcaaggcgg acttccgcaa
     1861 cgtaatcgtg atcatgacca ctaacgctgg cgctgaaaca gcggcgcggg catctattgg
     1921 ctttactcat caggaccact catccgatgc gatggaagtg atcaagaaga gcttcacgcc
     1981 agagttccgc aaccgcctgg ataccattat ccaattcggt cgcctgagcc acgaagtgat
     2041 taaaagtatc gtcgacaagt tcctcaccga gctgcaggct cagcttgagg ataagcgcgt
     2101 cacgctggag gtcagcgatg cggcgcgtaa ctggttggcg gaaagcggtt acgacgtgca
     2161 gatgggtgct cgtccgatgg cgcgcctgat ccaggacaag atcaagcgtc cgctggcgga
     2221 agagattctg tttggcgagc tggctgagca cggtggtgtg gtgcatatcg acattgaagg
     2281 tggtgagctg accttcgact ttgagaccac ggcagaaatg gcctaggcgt agtttcttgc
     2341 gcacacaaaa acgcccggca tgccgggcgt ttttactgga gcttggtgta acagatgcca
     2401 ggaaaacgaa gttttgctgg cctgttaacg ggcgcgataa gtaatgcggc ccttgctcaa
     2461 gtcataaggc gtaagttcaa cacgaacttt gtcgccagtc agaatgcgga tgtaattttt
     2521 gcgcatcttt ccggagatgt gcgcagtaac gacgtgccca ttttccaact ccacacggaa
     2581 catggtgttg ggcagggtgt cgacgacagt gccttccatt tcg
//
LOCUS       DASRUM010000168         6357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_419128, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000168 DASRUM010000000
VERSION     DASRUM010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419128"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VFF22_10945"
     CDS             <1..314
                     /locus_tag="VFF22_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HZX17368.1"
                     /translation="VLGEGAKAGHLSYLGDAEIGARSNIGAGTITCNYDGANKHRTVL
                     GEDVFIGSNSALVAPLTLGDGATTGAGSTITADVPDRALAVGRAKQRTIEGWKRPKKQ
                     P"
     gene            530..1303
                     /locus_tag="VFF22_10950"
     CDS             530..1303
                     /locus_tag="VFF22_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HZX17369.1"
                     /translation="MVSKRNTPQRRHTILALLAEQGEVSVDALSKAFATSEVTIRKDL
                     AALEKNGLLLRRYGGAVPMPQELIGDGAQPVSAYKQAIARAGVARIREHARIIIDSGT
                     TTAAMIPELGHKPGLVVMTNSLHVARALSELEHEPVLLMTGGTWDPHSESFQGQVAEQ
                     VLRSYDFDQLFIGADGIDLARGTTTFNELLGLSRVMAEVAREVIVMVEADKIGRKIPN
                     LELPWSGIHTLITDERLAPEAREQITARGVNLICAAVDN"
     gene            1316..3166
                     /gene="glmS"
                     /locus_tag="VFF22_10955"
     CDS             1316..3166
                     /gene="glmS"
                     /locus_tag="VFF22_10955"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZX17370.1"
                     /translation="MCGIVGAVAERNITAILLEGLKRLEYRGYDSAGVAVFSKAGSLE
                     RRRRVGKVAELEQALAAEPLAGRLGIAHTRWATHGAPSERNAHPHFSGSDLAVVHNGI
                     IENHDALRAQLKELGYVFSSDTDTEVIVHLLEYKLKSLGDLTAALKAAVKELHGAYGL
                     AVVSAAQPDRLLAARSGSPLVIGLGLGENFLASDQLALRQVTDRFMYLEEGDIAEIQR
                     DSVRIWAADGTPVLRESVQYHEGAEAADKGEYRHFMLKEIHEQPKVVQRTLEGRLGSD
                     HVLVQAFGPQAAELFARVRNVQIVACGTSYHAGMVARYWLEGLAGIPCQVEVASEFRY
                     RKVVVQPDTLFVSISQSGETADTLAALRNGKALGGENGGYLASLAICNVGISSLVRES
                     DLCLLTLAGPEIGVASTKAFTTQLVALLLLTLSLGQVRGTLEAGVEAELVEELRRLPT
                     RLGEALAMDGTVEKIAELFAEKHHALFLGRGAQYPVALEGALKLKEISYIHAEAYPAG
                     ELKHGPLALVDSDMPVVTVAPNNELLEKLKSNLQEVRARGGELIVFADREAGLSNGEG
                     THVVAMPHILDALAPILYTIPLQLLSYYVAVLKGTDVDQPRNLAKSVTVE"
     gene            3390..4577
                     /locus_tag="VFF22_10960"
     CDS             3390..4577
                     /locus_tag="VFF22_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003178532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HZX17371.1"
                     /translation="MIASKNYLDPVLPERLPDSIIRAADDLPRKTEFLAGRLPRQTAE
                     QLVSLLRLTNSYYSNLIEGHRTEIAQLQEARTAPKRARKQLTDLAAQHMDQQEVMERA
                     LRARPQAAFADMFDPHLISMIHKRLFKGASLQDLMLGDGRLMVPGKLRSEENEQVQVG
                     VHLAPVAAVVEPMLAHLQLHFGRIRDPRQRLVAVLANHHRLSFIHPFLDGNGRVARML
                     THLQLAYLGLQPVLWSLSRGLARRQDDYYRYLAMADRSREGDIDGRGQLSQRRYFEFI
                     EFMLDVCHDQVRYMSAALDTAALRERVTQVFASDPELRRQGIRPTSAPAVLALLTQGT
                     LPRAEFKLFTGLKDRLATEELGRLIEAGIVISRSPRARAVEVGLPARFAGLIFADLHF
                     HMA"
     gene            complement(4589..4816)
                     /locus_tag="VFF22_10965"
     CDS             complement(4589..4816)
                     /locus_tag="VFF22_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17372.1"
                     /translation="MNGKNEPVEAFEPLGLSAELSAAQREELEQYDDALQHYRTRRWE
                     LAEQSLQQLRSVHPQTKLYRIYLERIRYRSW"
     gene            complement(4843..>6357)
                     /gene="pgi"
                     /locus_tag="VFF22_10970"
     CDS             complement(4843..>6357)
                     /gene="pgi"
                     /locus_tag="VFF22_10970"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012312664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HZX17373.1"
                     /translation="GLFLDYSKNLINTGTRELLFGLAMEAGLQQSIRQLFKGALVNAS
                     EQRPALHTALRRPIGDQVLVDGVDVMPEVQRVLNQMSELVARVHNGLWRGYSEKPITD
                     VVNIGIGGSFLGPQLVSEALLPFAQRGVRCHYLANIDGSSFHELAAKLRAETTLFIVS
                     SKTFNTLETLKNAQAARSWYLAQGGSEAELHRHFMAVSSNTQAALAFGIREENILPMW
                     NWVGGRYSLWSAIGLPIAMSIGISNFKELLAGAYRMDEHFQNAPFERNMPVLLALLGI
                     WYGNFWGAQSHAILPYDHYLRNITKHLQQLDMESNGKHVRQDGSAVTCATGPVIWGGV
                     GCNGQHAYHQLLHQGTQLIPADFIVPVSSYNPVADHHQWLFANCLSQSQALMLGKSRE
                     EAEAELRAQGLDEDQVQHLSPHKAIPGNRPSNTLVLERINPGRLGALIALYEHKVFAQ
                     SAIWGINAFDQWGVELGKELGKGVYSRLVGCEKELAEDGSTQGLIDFFRAHHRG"
BASE COUNT         1188 a         2037 c         1999 g         1133 t
ORIGIN      
        1 ccgtgctggg cgagggcgcc aaggccggcc acctgagcta tctgggcgat gccgaaatag
       61 gcgcgcgcag caatatcggc gccggcacca tcacctgcaa ctacgacggc gccaacaagc
      121 accgcaccgt actgggcgag gacgtcttta tcggctccaa cagcgccttg gtcgctcccc
      181 tgaccctggg cgatggcgcc accaccggcg ccggctcgac catcaccgcc gacgtgccgg
      241 accgggccct ggcggtcggc cgggccaagc agcgcaccat cgaaggctgg aagaggccaa
      301 aaaagcagcc ataagcctta agagcagccg caagcggcaa gccataagca taagcgccaa
      361 gcaagagcaa aaaaccaagc gacagcttga ggctgcggga gtaaccctca tcctgagcag
      421 cttgcagctt gcagcttgca gcttgcagct tgcagcttgc agcttgcagc ttgcagcttg
      481 cagctgcttt atgttttgat tcgcgaaatt atctttcgaa tcgaaactta tcgtgtcgaa
      541 acgcaacaca ccgcaacggc gccacaccat actcgccctg ctcgccgagc agggcgaggt
      601 gagcgtcgat gcgttgtcga aggccttcgc cacctcggaa gtgaccatcc gcaaggacct
      661 cgccgccctg gaaaagaacg gcctgctgtt gcgccgctac ggcggcgcgg tgcccatgcc
      721 ccaggagttg atcggcgacg gcgcgcagcc ggtttccgcc tacaagcagg ccattgcccg
      781 tgccggcgtg gcgcgtatcc gcgagcacgc gcggatcatc atcgacagcg gcaccaccac
      841 ggcggcgatg atccccgagc tggggcacaa acccggtctg gtggtgatga ccaactcgct
      901 gcacgtcgcc cgtgccctca gcgaactgga gcacgagccg gtgctgctca tgaccggcgg
      961 cacctgggac ccccactcgg aatccttcca gggtcaggtc gccgagcagg tgctgcgctc
     1021 ctacgatttc gaccagctgt ttatcggcgc cgatggcatc gacctggcgc gcggtaccac
     1081 caccttcaac gaactgctgg ggttgtcgcg ggtcatggcc gaggtcgccc gggaagtcat
     1141 agtcatggtc gaagccgaca agatcggccg caagataccc aacctggagc tgccctggag
     1201 cggcatccat accctgatta ccgatgagcg cctcgccccc gaggcgcgtg aacagataac
     1261 ggcgcgcggg gtgaacctga tttgcgcggc cgtggataac taaggagatc tccccatgtg
     1321 tggcatcgtt ggcgccgtcg ccgaacgcaa catcaccgcg attctgctgg aaggcctgaa
     1381 gcgcctggaa taccgcggtt acgacagcgc cggcgtggcg gtcttcagca aggccggcag
     1441 cctggagcgc cggcgccggg tcggcaaggt ggccgagctg gagcaggccc tggccgccga
     1501 gcccttggcc gggcgcctgg gcatcgccca cacccgctgg gccacccacg gcgcgcccag
     1561 cgagcgcaac gctcacccgc acttctccgg tagcgatctg gcggtggtgc acaacggcat
     1621 tatcgagaac cacgacgccc tgcgcgccca gctcaaggag ctgggttatg tcttcagctc
     1681 cgataccgac accgaagtta tcgtccacct gctcgagtac aagctcaaaa gcctgggcga
     1741 cctcacggcg gccctgaaag ccgcggtcaa ggaactgcat ggcgcctatg gcctggcggt
     1801 ggtcagtgcc gcccagcccg accgactgct cgccgcccgt agcggcagcc cgctggtgat
     1861 cggcctgggc ctgggggaaa acttcctggc ctcggaccag ttggcgttga gacaagtcac
     1921 tgaccgcttc atgtacctgg aagagggcga tatcgccgag atccagcgcg acagcgtgcg
     1981 gatctgggcg gccgacggca cccctgtgct gcgtgaaagc gtgcagtacc acgagggcgc
     2041 cgaggcggcg gataaaggag agtatcgcca ctttatgctc aaggaaatcc acgaacagcc
     2101 caaggtggtg cagcgcaccc tggaaggccg cctgggttcc gaccatgtgc tggtgcaggc
     2161 ctttggcccc caggccgccg agctgttcgc ccgggtgcgc aacgtgcaaa tcgtcgcctg
     2221 cggcaccagt taccatgccg gcatggtcgc ccgttactgg ctcgaaggcc tggccgggat
     2281 tccctgtcag gtggaagtgg ccagcgagtt ccgctaccgc aaggtggtgg tgcagccgga
     2341 caccctgttc gtcagcatct cccagtccgg cgagaccgcc gataccctgg cggcgctgcg
     2401 caacggcaag gcgctgggcg gcgagaacgg cggctacctg gccagcctgg ccatctgtaa
     2461 cgtcggcatc agctccctgg tgcgcgaatc cgacctgtgc ctgctgaccc tggccggccc
     2521 ggaaatcggc gtggcctcga ccaaggcctt caccacccag ctggtcgccc tgctgctgct
     2581 gaccctgtcc ctcggccagg tgcgcggcac cctggaagcc ggggtcgaag ccgagctggt
     2641 ggaagagctg cgccgcctgc cgacccgctt gggcgaggcc ctggccatgg acggcacggt
     2701 ggagaagatc gccgaactgt tcgccgaaaa acaccacgcg ctgttcctcg gccgtggcgc
     2761 ccagtacccg gtggcactgg aaggcgcgct caagctcaag gaaatctcct atatccatgc
     2821 cgaggcctac ccggccggcg agctcaagca cggccccctg gccctggtgg acagcgacat
     2881 gccggtggtc acggtggcgc cgaacaacga gctgctggaa aagctcaagt ccaacctgca
     2941 ggaagtgcgt gcccgcggcg gcgagctgat cgtgttcgcc gaccgggaag ccggcctgag
     3001 caacggcgag ggcacccatg tggtggccat gccgcatatc ctcgatgccc tggcaccgat
     3061 tctctacacc atcccgctgc agctgctgtc ctactacgtc gccgtgctca agggcaccga
     3121 cgtcgaccag ccacgcaacc tggccaagag cgtcacggtc gagtaagccg cgacccagcc
     3181 atgaacaagc ccggccacta gccgggcttt tttctagccg attgtcgtcg acttaggtgt
     3241 cgaaattttt ccgcagaaaa cttcagattg gtgtgtcaag ttttcacgta aacccataaa
     3301 ttacgggcta tataagggga taaattatcc gcaaaaattt ccgcagaaag tgcgctatag
     3361 ttttactcga acgatcctga gcctgccgaa tgatcgcgtc gaaaaactac ctcgatcccg
     3421 tgttgccaga aaggctgcct gattcgatca tccgggccgc ggatgatctg cctcgcaaga
     3481 ccgagttcct ggcaggtcgg ctgccgcggc aaacggcgga gcaactggtc agcttgcttc
     3541 gtctgaccaa cagctactac tcaaacctga tcgaagggca tcggacggaa atcgcccaac
     3601 tccaggaagc tcgcacggcg cccaagcgcg cacgaaagca gctcacggat ctggcggcgc
     3661 agcatatgga ccagcaggaa gtcatggaac gtgcgctacg agctcggccg caggcagcgt
     3721 tcgcggacat gttcgatccc catctgatct ccatgatcca caagcgactg ttcaagggcg
     3781 catccctgca ggacctcatg cttggagatg ggcgcctgat ggtgccgggc aaactgcgct
     3841 ccgaggagaa cgagcaggtc caggttggtg tccatctggc gcctgtggct gcggtggtcg
     3901 agccaatgct ggctcaccta cagctgcact tcggacgtat ccgagaccca cgtcagcggc
     3961 ttgttgcggt actggccaat catcaccgct taagcttcat ccacccgttc ctggacggga
     4021 atgggcgggt tgcgcgcatg ctgacccacc tgcaactggc gtacctgggt ttgcagcccg
     4081 tgctctggtc gctttcccga ggactggcac gccgccagga tgactactac cgctacctgg
     4141 ccatggctga ccggtcgcgg gagggcgata tcgatggtcg aggtcagctg tcccagcgcc
     4201 gctacttcga gttcatcgaa ttcatgctcg atgtctgcca cgaccaggtc cgctacatga
     4261 gcgccgcgct cgacacggca gcgctgcgcg agcgggtgac gcaggtgttc gctagcgatc
     4321 ctgagttgcg gcgccagggc atccggccga ccagcgcgcc agccgtgctg gcgctgctca
     4381 cccaagggac gctgccacgc gcggagttca agctgttcac aggcctgaaa gaccgcctcg
     4441 ctaccgagga gcttggccgg ctgattgaag cggggatagt gatcagccga tcgcccaggg
     4501 cccgcgcagt ggaagtggga ctgcctgccc ggttcgccgg ccttatcttt gcggatctgc
     4561 atttccatat ggcttgaata ccggggtctt accagctgcg gtagcggata cgctccaggt
     4621 agatacgata cagcttggtc tgaggatgga cgctgcgcag ctgttgcaag ctttgctcgg
     4681 ccaactccca gcggcgcgtg cggtagtgtt gcaaggcgtc atcgtattgt tcgagttctt
     4741 ctcgctgcgc ggcgctcaac tcggcactaa gacccagcgg ttcgaaagcc tcgacaggtt
     4801 cgttcttgcc gttcaccagc aggcggtctt ggcagcgccc tgtcagccgc gatggtgggc
     4861 gcggaagaaa tcgatcaagc cctgggtcga gccatcttcc gccagttctt tttcgcagcc
     4921 gaccaagcgt gaatacacgc ccttgcccag ctccttgccg agctccacgc cccactgatc
     4981 gaaggcgttg atgccccaga tagcgctctg ggcgaatacc ttgtgctcat agagggcgat
     5041 caatgctccc aggcgaccgg ggttgatgcg ttcgagtacc agggtattgc tcggccggtt
     5101 gccggggatc gccttgtgtg ggctcaggtg ctgcacctgg tcttcgtcca ggccctgggc
     5161 gcgcagttcg gcttcagctt cctcgcgcga cttgccgagc atcagtgcct ggctttgcga
     5221 caggcagttg gcgaacagcc actgatgatg gtcggcgacc gggttgtagc tgctcaccgg
     5281 tactatgaaa tccgccggaa tcagctgggt gccctgatgc agcagctggt gatacgcgtg
     5341 ttgaccgttg cagccgaccc cgccccagat taccgggccg gtggcgcagg ttaccgcgct
     5401 accgtcctgg cgcacgtgct tgccgttgga ctccatgtcc agctgttgca ggtgcttggt
     5461 gatgttccgc aggtagtggt cgtagggcag gatggcgtgg ctctgcgcgc cccagaagtt
     5521 gccgtaccag atgcccagca aggccagcag caccggcata ttgcgttcga agggcgcgtt
     5581 ctggaaatgc tcgtccatgc ggtaggcacc tgcgagcaat tccttgaagt tggagatgcc
     5641 gatcgacatg gcgatcggca ggccgatggc cgaccacagc gaataacgcc ctccgaccca
     5701 gttccacatc ggcaggatgt tttcctcgcg aatgccgaac gccagcgccg cctgggtatt
     5761 gctggagacc gccataaagt gccggtgcaa ttctgcctcg gagccgccct gggccaggta
     5821 ccagctgcgc gctgcctggg cattcttcag ggtttccagg gtattgaagg tcttcgacga
     5881 gacgatgaac agggtggttt ccgcgcgcag cttggcagcc agctcgtgga aggaactgcc
     5941 atcgatattc gccaggtagt ggcagcgcac gccacgctgg gcgaacggca gcaaggcctc
     6001 ggacaccagt tgcggaccga ggaaggagcc gccgatgccg atattcacca cgtcggtgat
     6061 cggcttctcg ctgtagccac gccacaggcc gttatgcacg cgggcgacca gctcgctcat
     6121 ctggttgagc acacgctgca cctcgggcat cacatcgacg ccatcgacca gcacctggtc
     6181 gccgattggc cggcgcaatg cggtgtgtag ggccggacgc tgttccgagg cgttgaccag
     6241 ggcacccttg aacaactgtc gaatcgactg ttgcaggccc gcctccatgg ccaggccgaa
     6301 tagcagctcg cgggtgccgg tgttgatgag gtttttcgag tagtcgagaa acaggcc
//
LOCUS       DASRUM010000169         4835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_252400, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000169 DASRUM010000000
VERSION     DASRUM010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252400"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /gene="ftsZ"
                     /locus_tag="VFF22_10975"
     CDS             complement(<1..867)
                     /gene="ftsZ"
                     /locus_tag="VFF22_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZX17374.1"
                     /translation="MFELVDSAPASAVIKVIGVGGGGGNAVNHMAKNNIEGVEFICAN
                     TDAQALKNIGARTVLQLGTAVTKGLGAGANPEVGRQAAMEDRERIAEVLQGTDMVFIT
                     TGMGGGTGTGAAPVIAQVAKEMGILTVAVVTRPFPFEGKKRMLIAEEGIRALSESVDS
                     LITIPNEKLLTILGKDASLLSAFAKADDVLAGAVRGISDIIKRPGMINVDFADVKTVM
                     SEMGMAMMGTGCASGPNRAREATEAAIRNPLLEDVNLQGARGILVNITAGPDLSLGEY
                     SDVGNIIEQFASE"
     gene            complement(912..2159)
                     /gene="ftsA"
                     /locus_tag="VFF22_10980"
     CDS             complement(912..2159)
                     /gene="ftsA"
                     /locus_tag="VFF22_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HZX17375.1"
                     /translation="MANVQSGKMIVGLDIGTSKVVALVGEVNADGQLEIVGIGTHPSR
                     GLKKGVVVNIESTVQSIQRAVEEAQLMAGCRIHSAFVGVAGNHIRSLNSHGIVAIRDR
                     EVSPADLERVLDAAQAVAIPADQRVLHTLPQDYVIDNQEGVREPLGMSGVRLEAKVHV
                     VTCAVNAAQNIEKCVRRCGLEVDDIILEQLASAYAVLTDDEKELGVCLVDIGGGTTDI
                     CIFTEGAIRHTAVIPIAGDQVTNDIAMALRTPTQYAEEIKIRYACALAKLAGAGETIK
                     VPSVGDRPPRDLSRQSLAEVVEPRYDELFTLIQAELRRSGYEDLIPAGIVLTGGTAKM
                     EGAVELAEEIFHMPVRLGMPQGVKGLADVVRNPIYSTGVGLLLYGLQKQADGISMSAL
                     GSYNDEPKQPVLERLKRWVQGNF"
     gene            complement(2180..3055)
                     /locus_tag="VFF22_10985"
     CDS             complement(2180..3055)
                     /locus_tag="VFF22_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HZX17376.1"
                     /translation="MTATLRHQPGSNRAPVRGKPAQRGASRLVAKEPISLRISMPKAR
                     FTGMGSWLKRLTWPLLLLALGYGTYELSLRALPYADQPIARISVEGDLSYVNQQAVQE
                     RIAPYASASFFSVDLLSLRRELELMPWIASAQVRRVWPDQLVVRLEEQLPIARWGDEA
                     LLNNQGEAFAPQQLGDYPHLPQLYGPKRAQQQVMQQYQVLSQMLRPMGFSVARLELRE
                     RGSWFLSTGQGIELLLGRDHLVEKMRRFTAIYDKALKEQQTNIARIDLRYANGLAVAW
                     REPVAPTAAPTAVVQ"
     gene            complement(3059..4009)
                     /locus_tag="VFF22_10990"
     CDS             complement(3059..4009)
                     /locus_tag="VFF22_10990"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HZX17377.1"
                     /translation="MSAASLQSQFPVSAFGRVAVLFGGKSAEREVSLKSGNAVLQALL
                     AAGVNAFGIDAGDDLLQRLTAEKIDRAFIVLHGRGGEDGSMQGLLECAGIPYTGSGIL
                     ASALAMDKLRTKRVWLSLGLPTPAHAALTCEADCHAAAAELGFPLFVKPAHEGSSIGM
                     AKVNDVDALIAAWKDASRYDSQVLVEQMIAGPEFTIAMLRGQVLPPIALGTPHSFYDY
                     DAKYLANDTQYRIPCGLSAEKEAELKELTARACEAVGTQGWARADVMQDADGKFWLLE
                     VNTVPGMTDHSLVPMAARAAGLDFQQLVLAILADSLEARG"
     gene            complement(4006..>4835)
                     /locus_tag="VFF22_10995"
     CDS             complement(4006..>4835)
                     /locus_tag="VFF22_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003260781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HZX17378.1"
                     /translation="LKKTYIEFLHNLPFYGLAVMCVDDPIVREILPQVARPTVTYGFD
                     EGADVRAINVRQQGMQTFFTVLRRDREPLDVSVNMPGNHNVLNALATIAIASDEGISD
                     EAIVQGLSQFQGVGRRFQVYGELPVDGGSVMLVDDYGHHPREVAAVIKAVRGGWPERR
                     LVMVYQPHRFSRTRDLYDDFVQVLGDAQVLLLMEVYPAGEAPIPGADSRNLCHSIRQR
                     GQLDPIYIERGVDLAPIVKPLLRAGDILLCQGAGDIGGLAPQLLQSPLFATAEGKTK"
BASE COUNT          906 a         1554 c         1437 g          938 t
ORIGIN      
        1 ctcggaagcg aactgctcga tgatgttacc cacgtcggag tactcaccca gggacaggtc
       61 aggaccggcg gtgatgttca ccaggatgcc gcgcgcgcct tgcaggttga cgtcttccag
      121 cagcgggtta cggatcgccg cttcggtggc ttcacgtgca cggttcggac cgctggcgca
      181 gccagtgccc atcatcgcca tgcccatttc gctcatcacg gttttcacgt cggcgaagtc
      241 aacgttgatc atgcccggac gcttgatgat gtcggagata ccgcgcacgg caccggccag
      301 tacatcgtca gccttggcga aagccgacag caggctggcg tccttaccga ggatggtcag
      361 cagtttttcg ttggggatgg tgatcagcga gtcgacgctt tccgacagtg cgcggatgcc
      421 ttcctcggcg atcagcatgc gcttcttgcc ttcgaacggg aacggacggg tcaccaccgc
      481 aacggtgagg atgcccattt ccttggccac ctgagcgata acaggcgcag caccagtacc
      541 ggtaccgccg cccatgccgg tggtgataaa gaccatatcg gtgccttgca gcacttcagc
      601 gatacgctcg cgatcttcca tggccgcctg acggccaact tctggattgg cacccgcgcc
      661 gaggcccttg gtcaccgcag tgcccagttg cagcacggta cgtgcaccga tgtttttcag
      721 tgcctgtgca tccgtgttgg cgcagataaa ctccacacct tcaatgttgt ttttcgccat
      781 gtgattgaca gcattaccgc cgccgccgcc cacgccgatg accttgataa ctgcactagc
      841 tggagcgctg tctacgagtt cgaacatttt ccctctcctt tactttctag ttgtaacgcc
      901 tactgcaaaa atcagaaatt gccctggacc cagcgtttca gccgttccag cacaggttgt
      961 ttgggttcat cgttgtagct gccaagtgca gacatggaga tgccgtcggc ctgcttttgc
     1021 agtccgtaca gcaacaggcc tacacctgtg gaataaatcg ggttacgcac cacatcagcc
     1081 aggcccttga cgccctgggg catgcccaga cgtaccggca tatggaagat ttcctcggcc
     1141 agttccaccg caccttccat cttcgccgta ccgccggtga ggacgatgcc cgctgggatc
     1201 aggtcttcgt agccactgcg gcgcagctcg gcctggatca gggtgaacaa ttcgtcgtaa
     1261 cgcggctcga ccacttcggc cagggactgg cgcgacaggt cgcgcggcgg acgatcacca
     1321 acgctcggca ccttgatggt ttcgccggca ccggccagtt tggccagagc gcaggcgtag
     1381 cggatcttga tttcttcggc gtactgagtc ggcgtacgca gggccatggc gatgtcgttg
     1441 gtcacttggt cgccagcaat aggaattacc gcggtgtggc ggatcgcgcc ttcggtgaag
     1501 atgcagatgt cggtagtgcc gccgccgata tccaccaggc acacgcccag ctctttctcg
     1561 tcgtcggtca gcaccgcata agcggaggcc agttgctcga ggatgatgtc gtcgacttcc
     1621 aggccacagc gacgcacaca cttctcgatg ttctgcgcgg catttactgc acaggtgacc
     1681 acgtgcacct tggcttccag acgcacgccg gacatgccca gcggctcacg cacgccttcc
     1741 tggttatcga tcacgtaatc ctgcggcagg gtgtgcagca cgcgctggtc ggcaggaatg
     1801 gccacggcct gagctgcgtc gagtacgcgc tccagatccg ccgggctgac ttcgcgatca
     1861 cgaatggcga cgatgccgtg gctgttgagg ctgcgaatat ggttgccggc cacaccgacg
     1921 aaggccgagt ggatacggca gccggccatc agctgggctt cttcaacagc acgctggatc
     1981 gactgcacgg tggattcgat attcaccacc acgccctttt tcaggccgcg cgacggatgg
     2041 gtgccgatgc cgacgatttc cagttggccg tccgcattca cttcaccgac cagcgccacc
     2101 accttggagg taccgatgtc gaggccgacg atcatcttgc cgctctgcac gtttgccatg
     2161 gctcttgcct tctcctgatt cactgcacga cagcggttgg tgccgccgtg ggcgctaccg
     2221 gctcacgcca tgccacggcc agaccgttgg cataacgcag atcgatccgc gcgatattcg
     2281 tttgttgctc tttcagggcc ttgtcataaa tggccgtgaa gcgccgcatt ttttccacca
     2341 gatggtcacg ccccagcagc agctcgatac cctgcccggt ggagaggaac cagctgccgc
     2401 gctcacgcaa ttcaaggcgc gccacggaaa agcccatcgg ccgcagcatc tggctgagca
     2461 cctggtattg ctgcatcacc tgctgctgcg cacgcttggg gccatacagc tgcggcaggt
     2521 gtgggtaatc ccccagctgc tgcggcgcga acgcctcgcc ctggttgttc agcagcgcct
     2581 catcgcccca acgggcaatc ggcaattgtt cttctagacg caccaccaac tgatccggcc
     2641 atacgcggcg cacctgggcg ctggcgatcc atggcatcag ttccagctcg cgtcgcaggc
     2701 tgagcagatc gacgctgaaa aagctggcgc tggcataagg cgcaatgcgc tcttgcacgg
     2761 cctgctgatt gacgtagctg aggtcgccct cgacgctgat ccgcgcaatc ggctggtcgg
     2821 cataaggcaa cgcgcgcaac gacagctcat aggtgccgta acccaatgcg agcagcaaca
     2881 gcggccaggt cagacgtttc agccagctgc ccatgcctgt gaaacgggct ttcggcatgc
     2941 ttatccgcag gctgatcggc tccttggcca ccaaacggct ggcgccgcgc tgcgcaggct
     3001 tgccgcgcac gggtgcgcgg ttcgagcctg gctggtgacg caaggtggcc gtcatgactt
     3061 aaccccgcgc ctcaagactg tcggcaagaa tcgccaacac cagttgttga aaatccagac
     3121 cggctgcacg ggccgccatc ggcaccaggc tgtggtcggt catgcccggc acggtgttga
     3181 cttccagcag ccagaacttg ccatcggcgt cctgcatcac gtctgcgcgg gcccaaccct
     3241 gggtgccgac cgcttcgcag gcgcgcgcgg tgagttcctt cagctcggct tccttctccg
     3301 ccgaaaggcc gcacgggatg cggtactggg tgtcattggc caggtacttg gcgtcgtagt
     3361 cgtagaagct gtgcggcgtg cccaacgcaa taggcggcag cacctgaccg cgcagcatgg
     3421 cgatggtgaa ttccgggccg gcgatcatct gctcgaccag cacctgcgag tcataacggc
     3481 tggcgtcctt ccaggcagca atcagcgcat cgacatcatt gaccttggcc atgccaatgc
     3541 tcgagccctc atgggccggc ttgacgaaca atgggaagcc cagctcggca gccgccgcat
     3601 ggcaatcggc ttcgcaggtc agcgcggcgt gagccggcgt cggcaggccg aggctcagcc
     3661 acacgcgctt ggtgcgcagc ttgtccatgg ccagagccga agccaggatg ccgctaccgg
     3721 tgtaaggaat cccggcgcat tcgagcagcc cctgcatcga gccgtcttcg ccaccacggc
     3781 cgtgcagcac gataaaggcg cggtcgattt tctcggcagt cagacgctgc agcaggtcat
     3841 cgccagcatc gataccaaag gcattcacac ctgcagccag cagcgcttgc agcacggcgt
     3901 tgccggactt cagcgagact tcacgctcgg cgctcttgcc gccgaacagc acggccacgc
     3961 gcccaaaggc gctgaccgga aattgtgatt gcagactggc agcactcatt tggttttacc
     4021 ttcagccgtt gcgaacaaag ggctttgcaa cagttgcggc gccaggccgc cgatatcacc
     4081 agcaccttgg caaagaagaa tgtcgcccgc gcgcagcagc ggcttgacga ttggcgcgag
     4141 gtcgacgccg cgctcgatat agatcgggtc gagctggcca cgctgacgaa tgctgtggca
     4201 caggttgcgg ctgtcagccc ctggaatcgg cgcttcgccg gccgggtaga cctccatcag
     4261 caacagcacc tgagcatcac ctagcacctg gacgaaatcg tcatacaggt cgcgggtgcg
     4321 gctgaaacgg tgcggctggt aaaccatcac caggcggcgc tccggccaac caccgcgcac
     4381 ggccttgatc accgcagcga cttcgcgcgg gtggtggccg tagtcatcga ccagcatcac
     4441 gctaccgccg tccaccggca gttcgccgta gacctggaaa cgtcgcccca caccctgaaa
     4501 ctgcgatagg ccctggacga tggcttcatc gctgatgcct tcgtcgctgg caatcgcgat
     4561 ggtggccagg gcgttcagta cgttgtggtt gcccggcatg ttcaccgata catccaacgg
     4621 ctcgcggtcg cggcgcagca cagtaaagaa ggtctgcata ccctgctgac gcacgttgat
     4681 cgcacgtacg tcggcacctt catcaaagcc gtaagtcacg gttggacggg ccacctgtgg
     4741 caggatctcg cgcacgatcg ggtcatccac acacatcacc gccagcccgt agaacggcag
     4801 gttgtggagg aactcgatgt aggttttctt caact
//
LOCUS       DASRUM010000170         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1270873, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000170 DASRUM010000000
VERSION     DASRUM010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270873"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1059)
                     /locus_tag="VFF22_11000"
     CDS             complement(133..1059)
                     /locus_tag="VFF22_11000"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZX17379.1"
                     /translation="MHIILLSRHHGAARSLSLDLRWLLLALLLALGAALASGVAVGAW
                     WAPATVKVHDDTQLNQAMDQQRAEVGEVRRDAQRQLDAFAVHVAELQARLTRLDALGA
                     RLTELADLDASEFDFSLNVGQGGAEDALGDAAYAPPPFMDELDRLSLRLDSREQLLEV
                     LEQLIGERQLNEAEYLSGMPVRHGYMSSPFGRRVHPLTGRNTQHKGVDFAAKPGSDVV
                     AVAAGVVSFSGVKSGYGNVVEISHADGYTTLYAHNKSNQVQIGDLVQRGQTIAKVGRS
                     GRASGYHVHFEVTKDGRLVNPASYIARVNDVE"
     gene            1141..1596
                     /locus_tag="VFF22_11005"
     CDS             1141..1596
                     /locus_tag="VFF22_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HZX17380.1"
                     /translation="MTFRPLPARAPAALLREAKPLKALFNQAQRIDRLQHLLESQLQP
                     AAREHCHVASWREGCLLLIITDGHWATRLRYQQKRLLKQLQALEEFATLTKILFKVQP
                     PTAERRAPGRTISLSSSAAENIQATADGISDPKLRAALERLAKHGKPSE"
     gene            complement(1676..2587)
                     /gene="lpxC"
                     /locus_tag="VFF22_11010"
     CDS             complement(1676..2587)
                     /gene="lpxC"
                     /locus_tag="VFF22_11010"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HZX17381.1"
                     /translation="MIRQRTLKNIIRATGVGLHSGEKVYLTLKPAPVDTGIVFRRTDL
                     DPVVEIPARAGNVGETTMSTTLVNGDVKVDTVEHLLSAMAGLGIDNAYVELSASEVPI
                     MDGSAGPFVFLIQSAGLQEQDAHKQFIRILREVTVEEGDKRATFVPFEGFKVSFEIDF
                     DHPVFRNRTQSASVDFSSTSFVKEVSRARTFGFMRDIEFLRSQNLALGGSVDNAIVVD
                     EFSVLNEDGLRYEDEFVKHKILDAIGDLYLLGNSLIGEFKGFKSGHALNNRLLRALIE
                     QTDAWEVVTFDDPNTAPISYMRPVAAV"
BASE COUNT          597 a          793 c          720 g          487 t
ORIGIN      
        1 caaaggcgca aacatatcga caggatcttc caggcgtatg gattgagggc ggctgagcag
       61 cgacccatgc agcctttatg gctgcgtgcg aagggggcat tctacccgga aacgcaaatg
      121 aggaaagcgg cgttattcga cgtcgttgac ccgggctata tagctggctg gattgaccaa
      181 ccgaccatcc ttggtcactt cgaaatgtac atggtaacca ctggcgcgac cgctgcggcc
      241 gaccttggca atggtctggc cgcgctggac cagatcaccg atctgcacct gattgctctt
      301 gttgtgcgca taaagtgtgg tgtacccgtc agcgtggctg atctccacga cattgccata
      361 acccgacttg accccagaga agctcaccac gccggccgcg acggccacta catcactgcc
      421 cggtttggca gcaaaatcga cccctttatg ctgagtatta cgcccggtca gtgggtgcac
      481 acggcgaccg aagggcgagg acatatagcc atggcgcaca ggcatgccgg agaggtattc
      541 ggcctcattc aactgacgct cgccgatcag ttgctccagt acttccagca actgctcacg
      601 actgtccagg cgcaggctca ggcgatcaag ctcatccata aacggcggcg gcgcatacgc
      661 ggcatcgccc agggcatctt cagcaccgcc ctgaccaaca ttgagagaga aatcaaattc
      721 gctggcatcc agatccgcca gctcggtgag gcgtgcgccc aaggcatcca gacgagtcag
      781 acgcgcctgc agctcggcca catgcacggc gaaggcatcc agttgacgct gagcgtcacg
      841 acgcacttcg ccgacttcgg cgcgctgctg atccatcgcc tgattgagct gggtgtcgtc
      901 gtgcacctta accgtcgcag gtgcccacca ggcaccaaca gccacaccgc tggccagcgc
      961 cgcgccgagc gccagcagca acgccagcaa taaccagcgc aggtccagac tgagcgaacg
     1021 tgcggcaccg tggtgccgac taagtagaat gatgtgcacg gccttcccct agtggtgaag
     1081 cgtcagtcgg cagcagcgat tctgctacca tggcgacctc actttttcag gcgtcctgcc
     1141 atgacttttc gtcctttgcc agcccgggct cccgccgcgc tgttgcgcga agcgaagccc
     1201 ctgaaggcgc tgttcaatca agcgcaacgc attgatcgcc tgcagcacct actggaaagc
     1261 cagctacagc ctgctgctcg cgaacactgc catgtagcat catggcgcga gggttgcctg
     1321 ctgctgataa tcaccgatgg ccactgggcc acgcgcctgc gctatcaaca aaaacgcttg
     1381 ttgaaacagc tgcaggcact cgaagaattc gcgaccttaa cgaagatcct attcaaggtg
     1441 caacctccga ctgccgaaag gcgtgcaccg ggtcgcacaa tcagcctgtc cagcagcgcc
     1501 gcggaaaaca tccaggcaac cgccgacggc atcagcgacc ccaagctacg cgccgccctg
     1561 gagcgcctgg ccaaacacgg caaacccagc gaataaatgc atcacccagt tactcgcaga
     1621 taaaaagagg ccacccgaag gtggcctcga aaagcaaagt aaggagggta ttgcgttaca
     1681 cggccgcaac tggacgcatg taagagattg gcgcggtgtt ggggtcatca aaggtgacga
     1741 cttcccacgc atcggtttgc tcgatcaggg cgcgcagcag gcgattgttc agcgcatgcc
     1801 ccgatttgaa acccttgaac tcgccaatca ggctgttacc cagcaggtaa agatcaccga
     1861 tcgcatcgag aattttgtgc ttaacaaatt cgtcctcata acgcaggccg tcttcgttca
     1921 acacgctgaa ctcatctacc acgatggcat tatctacgct gccgccgagt gccaggttct
     1981 gcgaacgcag gaactcgata tcacgcataa aaccgaaggt gcgagcgcga ctgacttcct
     2041 tcacaaacga ggtgctggag aaatcaacgc tggcagactg ggtgcgattg cggaacaccg
     2101 ggtgatcgaa gtcgatctcg aagctcacct tgaacccttc gaaaggcacg aaggtggcgc
     2161 gcttgtcgcc ctcttcaact gtcacttcac gaaggatacg gatgaactgc ttatgggcgt
     2221 cctgctcttg caaaccagca gattgaatca ggaatacaaa gggacccgca ctgccatcca
     2281 tgatcggcac ttcggatgcg gagagctcga cgtaggcgtt atcgatgccc aggccagcca
     2341 tggccgaaag caggtgctct accgtatcca ccttgacatc accattgacc aaggtggtcg
     2401 acatggtggt ttcaccaaca ttgcctgcac gcgcgggaat ttccacgaca gggtccaagt
     2461 cggtacgacg gaacacgata ccggtgtcca cgggtgccgg cttcagggtc aggtatacct
     2521 tttccccgga atgcaggccg acgccagtgg cgcggataat gttcttcagg gtgcgttgtc
     2581 tgatcatggc tttggcc
//
LOCUS       DASRUM010000171        10831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_253445, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000171 DASRUM010000000
VERSION     DASRUM010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 10831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10831
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253445"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFF22_11015"
     CDS             <1..219
                     /locus_tag="VFF22_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17382.1"
                     /translation="LQVGDRLFEIEKIRVHSHPSCVDGQVAGSCIRYRALPYSSRRIQ
                     VDTGLQVNRAAVQGVVTETRSLGSANQR"
     gene            complement(210..3731)
                     /gene="dnaE"
                     /locus_tag="VFF22_11020"
     CDS             complement(210..3731)
                     /gene="dnaE"
                     /locus_tag="VFF22_11020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZX17383.1"
                     /translation="MTASFVHLRLHTEFSLVDGLVRVKPLIKSVAAAGMPAVAVTDMS
                     NMCSLVKFYKAAMGGGIKPICGADIWLASSDEDGPLSRMTLLAMNAKGYRNLTELVSR
                     GWSEGQSNDLVIIQRDWVKEAAEGLIALSGAKEGEVGHALLDGEPEEAEAVLREWMEV
                     FPERFYLEVQRTNRVNDEEHLHAAVALADKVGTPLVATNDVRFIKQDDFAAHETRVCI
                     GEGRSLDDPRRLRTYSDQQYLKSPEEMAELFSDLPEALENTIEIAKRCNIEVQLGKYF
                     LPDFPVPAGMTMDEYFRQVSFEGLEERLAVLWPKETTPNYEEKRQVYIDRLNFELDII
                     IQMGFPGYFLIVMDFIKWAKNNGVPVGPGRGSGAGSLVAYVQKITDLDPLAYDLLFER
                     FLNPERVSMPDFDVDFCMDGRDRVIDYVAEKYGRNAVSQIITFGTMAAKAVVRDVARA
                     QGKSYGLADRLSKMIPFEVGMTLEKAYEMEEPLRDFLKVDEDAREIWEMSLKLEGICR
                     GTGKHAGGVVIAPTKLTDFSPIACDDEGGGLVTQFDKDDVESAGLVKFDFLGLRTLTI
                     IKWAMETINREQAKKGLPDLNIDFIPLDDKPTFSMLQKAETTAVFQLESRGMKELIKK
                     LKPDCLEDMIALVALFRPGPLQSGMVDDFINRKHGREPISYPHQDYQYAGLEPVLKPT
                     YGIILYQEQVMQIAQVMAGYTLGGADMLRRAMGKKKPEEMAKQRGGFIEGCASNGIDA
                     NLAGNIFDLVEKFAGYGFNKSHSAAYGLLSYQTAWLKAHHSAAFMAAVLSADMHNTDK
                     VVTLIEECRSMKLRLDAPDVNISEFKFTVNNEGWIVYGLGAIKGVGEGPVEAIVEARQ
                     AGPFKDLFDFCSRVDLKRINKRTLEALIRSGALDRLGPHFHEELKAYKANIDRNRAVL
                     LGAMEEAVQAAEQTARSAESGHMDLFGGVFADAEADVYLNHLRARELTLKERLKGEKD
                     TLGLYLTGHPIDEYEGEVRRFARQRIVDLKAARGDQTIAGLVVNLRVMKNKKGDKMGF
                     ITLDDRSGRIEASLFAEAFNSAQALLQTDALVVVEGEVSNDDFSGGLRLRAKRVMSLE
                     EARTGLADSLRLKVASSALQGDRLRWLADLCGRHRGACPITVDYSGNDAKAQLQFGEG
                     WRIEPADSLIQALRDQFGKDNVFLHYR"
     gene            complement(3843..4439)
                     /gene="rnhB"
                     /locus_tag="VFF22_11025"
     CDS             complement(3843..4439)
                     /gene="rnhB"
                     /locus_tag="VFF22_11025"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HZX17384.1"
                     /translation="MQLGLDFTLVQELVAGVDEVGRGPLCGAVVTAAVILDPLRPILG
                     LNDSKKLTEARREKLFDEIREKALSWCIARAEVEEIDQLNILHATMLAMQRAVEGLGI
                     TPKLALIDGNRCPILQMPSSAVVKGDSQVPAIAAASILAKVSRDREMQLLDQQYPGYG
                     IAGHKGYPTAVHLEALRRLGPTPIHRRSFGPVRALLDI"
     gene            complement(4439..5572)
                     /gene="lpxB"
                     /locus_tag="VFF22_11030"
     CDS             complement(4439..5572)
                     /gene="lpxB"
                     /locus_tag="VFF22_11030"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HZX17385.1"
                     /translation="MAALMRVALVAGEASGDILGAGLMQALKAQYPQIEFIGVGGPLM
                     QAQGLNSYFPMERLSVMGLIEVLGRLPELLSRRKRLINTLIEAKPDVFIGIDAPDFNL
                     TLELKLRQAGIKTVHYVSPSVWAWRQKRVLKIREACDLMLTLFPFEAQFYQDHQVPVR
                     FVGHPLADTIPQQADRAAAREALNLAHDHPVVALMPGSRGGEVSRLGSLFLDAAVRLR
                     TLRPGIQFVLPCSSPERRAQLEQMLVGRDLPLTLLNGRSHEALAACDAVLIASGTATL
                     EALLYKRPMVVAYKVAPLTYRILKRLVTSAYISLPNLLAERLLVPEMIQDAATPEALA
                     QLLAPLLDGGEVQTEGFDVIHRALRRDASVQAAQAVLQLTGRS"
     gene            complement(5576..6352)
                     /gene="lpxA"
                     /locus_tag="VFF22_11035"
     CDS             complement(5576..6352)
                     /gene="lpxA"
                     /locus_tag="VFF22_11035"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HZX17386.1"
                     /translation="MSLIDPRAIIDPSAKLADDVVVGPWSIIGPDVEIGEGSVLGPNV
                     IIKGPTRIGKHNRVYQFSSLGEDTPDRKYKGEPTRLVIGDHNIIREGVTMHRGTIQDR
                     DETTVGDHNLIMAYAHVGHDSVIGNHCILVNNVALAGHVHIGDWAILSGYTLVHQFCH
                     IGAHSFAGMGSAIGKDVPAYVTVSGNPAGARSMNFEGLRRRGFSAEAIAALRRAYKTV
                     YRQGLTIEAALAELAELALQFPEVAVFRDSILASQRGITR"
     gene            complement(6349..6789)
                     /gene="fabZ"
                     /locus_tag="VFF22_11040"
     CDS             complement(6349..6789)
                     /gene="fabZ"
                     /locus_tag="VFF22_11040"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HZX17387.1"
                     /translation="MMDINQIREYLPHRYPFLLVDRVAELDIEGKRIRAYKNVSINEP
                     FFNGHFPEHPIMPGVLIIEAMAQAAGILGFKMMDLKPSDGTLYYFVGSDKLRFRQPVV
                     PGDQLILEARYLSSKRSIWKFECKASVDGKEVCSAEIICAERKL"
     gene            complement(6838..7899)
                     /gene="lpxD"
                     /locus_tag="VFF22_11045"
     CDS             complement(6838..7899)
                     /gene="lpxD"
                     /locus_tag="VFF22_11045"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HZX17388.1"
                     /translation="MMSATVFTLGQLAERLGATLRGTADKSISGLATLQEAGADQLSF
                     LANAQYRKFLSGCQAGAVLLTAADADGYAGNALVVANPYLAYAELSHLFDRKPRALPG
                     VHPTAVVAADAQVDTSACIGAYAVIESGAQIAAGVTVGAQSVVGARSVIGEGGWLAPR
                     VTLYHDVRIGKRVVIQSGAVIGGEGFGFANEKGVWQKIAQIGGVTIGDDVEIGANTTV
                     DRGALSDTLIGNGVKLDNQIMIAHNVQIGDNTAMAGCAGISGSTKIGKNCMIAGGVGM
                     VGHIEVCDNVFVTGMTMVTRSITEPGSYSSGTAMQPAAEWRKSAARIRQLDDMARRLQ
                     QLEKQLAAVTPAADTSSDA"
     gene            complement(7899..8402)
                     /locus_tag="VFF22_11050"
     CDS             complement(7899..8402)
                     /locus_tag="VFF22_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HZX17389.1"
                     /translation="MRKLTQLVLLSVALLATPAFAEMKVAVLNYQMALLESDAAKRYA
                     VDAEKKFGPQLNKLKTLESDAKRIQDRLVKDGEKMQTAERERLELEFKQKARDFQFQS
                     KELNESKAVADREMLKKLKPNLDKAVEEVIKTGNFDLVLERGAVIDVKPQFDITRQVI
                     ERMNKLR"
     gene            complement(8446..10806)
                     /gene="bamA"
                     /locus_tag="VFF22_11055"
     CDS             complement(8446..10806)
                     /gene="bamA"
                     /locus_tag="VFF22_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HZX17390.1"
                     /translation="MKRLLLPAVLAALMITEVHAESFTISDIRVTGLQRVSAGSVFGA
                     LPLNVGAEADDRSLVEATRELFKTGFFQDIQLGRDGDVLVVTVVERPSISSIEIDGNK
                     AISSEDLLKGLNQSGLAEGEIFQRATLEGVRNELQRQYVAQGRYSAEIEAEVIPQPRN
                     RVALKININEGSVAAIQHINVVGNSVFSDEDLIDLFELKTTNWLSFFKNDDKYAREKL
                     SGDLERLRSYYLDRGYINMDISSTQVSITPDKKHVYVTVNVDEGEKYTVREVKLSGEL
                     KVPEEEVRALLLVQEGQVFSRKVMTTTSELITRRLGNEGYTFANVNGVPEAHDDGTVS
                     ITFVVDPGKRAYVNRINFRGNTKSEDEVLRREMRQMEGGWASTYLIDQSKTRLERLGY
                     FKEVNVETPQVPGTDDQVDVNYSVEEQASGSITASVGFAQNAGLILGGSITQNNFLGT
                     GNKVSVGLTRSEYQSNYNFGFVDPYWTPDGVSLGYNAFFRETDYDELNTDVSSYSVDS
                     YGVGASIGYPISDTSRLTYGLTIQNDSIGTGVYTVDEIFDFIEAEGDSYLNLKASVGW
                     SESTLNRGVLANRGHSQSLVFETTVPGSDLSFFKVDYRGQIFKPLSDTYTLRLHSQLG
                     YGDSYGSTEGLPFYENYYAGGFNSVRGFKDSSLGPRSTPSLGEKVTGNEGTLEDPDQD
                     PLPFGGNVLIQGGVELLFPMPFVKDQRSLRTALFWDVGNVFDTNCGKSQEECGSIDPG
                     NLASSVGVGLTWITALGPLSFSLAMPIIKPDDADTQIFQFSLGQTF"
BASE COUNT         2330 a         3287 c         3003 g         2211 t
ORIGIN      
        1 ttgcaggtcg gcgatcggct gttcgaaatc gagaaaattc gggttcatag tcatccgtct
       61 tgcgtcgacg gccaagtggc cgggagctgt atccgttatc gcgccctacc ttatagcagc
      121 aggcgcattc aggtcgacac cggactgcag gtaaaccgtg cagccgttca aggcgtcgta
      181 actgaaacgc ggagcctggg ctccgcaaat cagcggtagt gcaaaaagac gttgtctttg
      241 ccgaactggt cacgcaatgc ctgaatcaga ctgtcggccg gctcaatacg ccagccttca
      301 ccgaactgca attgcgcctt ggcatcgttg ccgctgtagt cgacggtgat cgggcacgcg
      361 ccacgatgac ggccgcacaa gtcggccagc caacgcagac gatcgccctg caaggcgctg
      421 ctggcgacct tcaaacgcag actgtcggcc aggccggtac gcgcttcctc caggctcatt
      481 acccgcttgg cgcgcaagcg caggccaccg gagaagtcgt cgttgctcac ctcgccctcg
      541 accaccacca aggcatcggt ctgcagcaag gcctgggcgc tgttgaacgc ttcggcaaac
      601 agggaggctt cgattcgccc ggagcggtca tcgagggtga taaagcccat cttgtcgccc
      661 ttcttgttct tcatcacccg cagattgacc accaggccgg caatcgtctg gtcaccgcgc
      721 gcagccttca ggtcgacaat gcgctggcgg gcaaaccgcc gcacttcgcc ttcgtactca
      781 tcaatcgggt gaccagtcag gtacaggccc agggtgtctt tctcaccttt gaggcgctct
      841 ttcaacgtca gctcgcgggc gcgcagatgg ttgagataga catccgcctc ggcatcggca
      901 aataccccgc cgaacaggtc catgtgcccg ctctcggcgc tgcgcgcggt ctgctcggcg
      961 gcctgtacgg cctcctccat cgcaccgagc aataccgcac gattacggtc gatgttggcc
     1021 ttgtaggctt tcagttcttc atggaagtgc ggaccaagcc ggtccaatgc accgctgcga
     1081 atcagcgcct caagggtgcg tttgttgata cgtttgaggt cgacccgcga gcagaagtcg
     1141 aacaggtcct tgaacggtcc tgcctgacgc gcctcgacga tagcctccac tggcccctcg
     1201 ccgacgccct tgatcgcgcc caggccgtag acgatccagc cctcgttgtt aaccgtgaac
     1261 ttgaattcgg agatgttcac atccggtgca tcgaggcgca gcttcatgct gcggcattcc
     1321 tcgatcaggg tgaccacttt gtcggtgttg tgcatatccg ccgacaacac cgccgccata
     1381 aaggccgccg agtggtgcgc cttgagccag gccgtctgat acgagagcaa gccataagca
     1441 gcggagtgag acttgttgaa accgtaaccg gcgaatttct ccaccagatc gaagatgtta
     1501 ccggccaggt tcgcatcgat gccgttgctg gcgcagcctt cgataaagcc gccgcgctgc
     1561 ttggccatct cctcgggttt cttcttaccc atggcgcggc gcagcatgtc cgcaccaccc
     1621 aaggtgtatc cggccatgac ctgagcgatc tgcatcacct gttcctggta caggataatg
     1681 ccgtaggtcg gtttcagcac aggttccagg cccgcgtact gatagtcctg atgcgggtag
     1741 gaaatcggct cgcgaccatg tttacggttg ataaagtcgt ccaccatacc cgactgcagc
     1801 ggccccggac ggaacagggc caccagagcg atcatgtctt ccaggcagtc tggcttgagc
     1861 ttcttgatca gctccttcat gccacgcgat tcaagctgga acaccgcagt ggtctcagct
     1921 ttctgcagca tgctgaatgt cggcttgtca tccagcggga taaagtcgat gttcaggtcc
     1981 ggcaggccct tcttggcctg ctcgcggttg atggtttcca tcgcccactt gatgatggtc
     2041 agggtacgca ggccgaggaa gtcgaacttc accaggccgg cggattccac atcgtcctta
     2101 tcgaactggg tgaccaggcc gccgccctca tcatcgcagg caatcggcga gaagtcggtg
     2161 agtttggtgg gcgcgatcac cacgccaccg gcgtgcttgc cggtaccgcg acagatgccc
     2221 tcgagcttga gcgacatttc ccagatttcc cgggcgtcct cgtcgacctt gaggaagtcg
     2281 cgcagtggct cctccatctc gtaggctttt tccaaggtca tgccgacttc gaaaggaatc
     2341 atcttcgaca ggcggtccgc tagcccgtag gacttaccct gggcccgcgc cacgtcgcgc
     2401 actaccgcct tggccgccat ggtgccgaag gtgatgatct ggctcaccgc attgcggccg
     2461 tacttctcgg ctacgtaatc gattacccgg tcgcggccgt ccatgcagaa gtcgacgtcg
     2521 aagtcaggca tggatacccg ttccgggttg aggaaacgtt cgaacagcag gtcataggcc
     2581 aacgggtcga ggtcggtgat cttctgcacg taagccacca gcgaaccagc acccgaccca
     2641 cggccaggac cgaccggcac gccgttgttc ttcgcccact taatgaagtc cattacgatc
     2701 aggaagtaac cggggaagcc catctggatg atgatatcca gctcgaaatt caaccggtcg
     2761 atatagacct gacgcttctc ttcgtaattc ggcgtggtct ccttcggcca gagcactgcc
     2821 aggcgctctt ccagcccctc gaacgagacc tggcgaaaat attcatccat ggtcatgccc
     2881 gccggtactg ggaagtccgg caggaagtat ttgcccagct gcacttcgat attgcagcgc
     2941 ttggcaatct cgatggtgtt ttccagggct tccggcagat cactgaacag ctcggccatt
     3001 tcctccgggc ttttcaggta ctgctgatcg gaataggtgc gcagacggcg cgggtcatcc
     3061 aggctgcgac cttcgccgat gcacacacgg gtttcgtggg cggcaaagtc gtcctgcttg
     3121 ataaagcgca catcgttggt tgccaccaac ggcgtgccga ccttatcggc cagagcgaca
     3181 gcggcgtgca gatgctcttc gtcattgacc cgattggtgc gctgcacttc caggtagaaa
     3241 cgctcgggga atacctccat ccactcgcgc agcacggcct cggcttcttc cggctcgcca
     3301 tcgagcaagg catggccgac ttcaccttcc tttgcgccgg acagggcaat caggccttcg
     3361 gccgcttcct tgacccagtc acgctgaatg atcaccagat cattgctctg cccttcgctc
     3421 cagccgcgcg agaccagttc ggtaaggttg cgataaccct tggcattcat cgccagcagg
     3481 gtcatgcggc tcagcgggcc atcttcatcg gaactggcca gccagatgtc ggcgccgcag
     3541 atcggcttga tgccgccgcc catggcggcc ttatagaact tcaccaatga gcacatgttg
     3601 ctcatatcgg taaccgccac cgctggcatg ccggctgccg ccaccgattt gatcagcggt
     3661 ttgacccgca ccagcccgtc gaccagggaa aactcggtgt gcaaacgcag gtggacgaat
     3721 gaagcggtca tggcagtcct atacaaaacg caaaaacgac aaggcaggga ttgtacaacc
     3781 gcagcagcgc acgacaagcg ccagcggaca caggataacg agggaatttc ttgggcgttg
     3841 cgtcagatgt ccagcaacgc acggaccgga ccaaaagagc gccgatgaat cggcgtcggc
     3901 ccaagacgcc gcaaggcttc caggtggaca gcggtgggat agcctttgtg cccggcaatg
     3961 ccataaccgg ggtattgctg gtcaagtagc tgcatctcgc gatcacggct gaccttggcc
     4021 agaatggatg ccgcagcaat cgccggcacc tggctatcgc ccttgactac cgcagagctg
     4081 ggcatctgca agatcgggca gcgattgcca tcgatcaatg ccagctttgg cgtgatgcct
     4141 aagccttcga ccgcgcgctg catggccagc atggtggcgt gaagaatatt cagctgatcg
     4201 atctcttcca cttcggcgcg ggcgatacac caggacaagg ctttttcgcg aatctcgtcg
     4261 aagagttttt cccggcgtgc ctcagtcagc tttttcgaat cgttcagccc gagaatcggg
     4321 cgcaacggat caagaatcac cgccgccgtc accacggcgc cacagagcgg accacggccg
     4381 acttcatcga cacccgccac cagctcctgt accagggtga aatccagccc gagttgcatc
     4441 agctgcgacc tgtcagctgc agcacagcct gagcggcttg caccgaggca tcacggcgca
     4501 gcgcgcggtg aatcacatca aagccttcgg tctgtacttc gccaccatcc agcaatggcg
     4561 ccagcaactg ggccaatgct tcaggcgtgg cagcgtcctg aatcatctca ggcaccagca
     4621 aacgctcggc cagcagattc ggcagggaaa tataagcgct ggtaaccagg cgcttgagaa
     4681 tgcgataggt caacggcgca accttgtaag ccaccaccat cggccgtttg tagagcaggg
     4741 cctcaagggt cgccgtgccg gaggcgatca gcaccgcatc acaggcggcg agcgcctcat
     4801 gggaacggcc attgagcaag gtcagcggca gatcgcggcc caccagcatc tgctcaagct
     4861 gcgcgcggcg ctccgggctg gaacacggca gaacaaactg gataccaggg cgcaaggtgc
     4921 gcaaccgcac ggcggcatcg agaaacaggc tgcccagacg cgacacttcg ccgccacggc
     4981 tgcccggcat gagggcaact accgggtgat catgcgctag gttcagcgcc tcacgggccg
     5041 ctgcgcgatc cgcctgctgt gggatcgtat cggccagggg gtggccgaca aaacgcaccg
     5101 gcacctggtg atcctgatag aactgcgcct caaacggaaa cagggtcagc atcaggtcgc
     5161 aagcctcgcg aatcttcagc acgcgcttct gccgccaggc ccacacggac gggcttacgt
     5221 aatgcacggt cttgataccc gcctgacgca acttgagttc aagggtcaga ttgaaatccg
     5281 gcgcatcgat gccgataaac acgtccggtt tggcctcgat cagggtgttg atcagccgct
     5341 tgcgccgtga cagcagctca ggcagacggc ccagcacctc gatcagcccc atcaccgaca
     5401 gacgctccat cgggaaatag gaattcagcc cctgagcctg catcagcggg ccaccgacac
     5461 cgataaattc gatctgtggg tattgggctt tgagggcctg catcaggccc gcgccgagaa
     5521 tatcgccgga agcctcgcca gccactagcg cgacacgcat tagagccgcc ataagttagc
     5581 gagtaatgcc gcgctgggag gccaggatag agtcgcggaa caccgcgact tcaggaaact
     5641 gcagggcgag ctcggccagt tcggccaatg ctgcctcaat ggttagcccc tggcgataca
     5701 ccgtcttgta ggcgcgacgc agagcggcga tcgcctcggc gctgaagcca cggcggcgca
     5761 gtccttcaaa gttcatgctg cgcgcccccg ccggattgcc ggaaacagtg acgtaggcag
     5821 gcacatcctt gccgattgcg ctgcccatgc cagcgaagct gtgcgcaccg atatggcaga
     5881 actgatgcac cagggtgtag ccggacagaa tcgcccagtc accgatatgt acatgaccgg
     5941 ccagcgcgac attgttgacc aaaatgcaat ggttgccgat tacgctgtca tggccgacat
     6001 gtgcataggc catgatcagg ttgtggtcac ccacggtggt ctcatcgcga tcctggatgg
     6061 tgccccggtg catggtcaca ccttcgcgga tgatgttgtg atcgccaatc accagtcgcg
     6121 tcggctcacc cttgtacttg cgatcagggg tgtcctcgcc caacgaggaa aactgataga
     6181 cgcggttgtg cttaccaatt ctggttggcc ctttgatgat cacattaggc ccaagcacgc
     6241 taccctcacc aatttccaca tcaggcccga tgatcgacca agggccgacg acgacgtcgt
     6301 cggccagctt ggcactgggg tcaataatgg cgcgagggtc aatcaaactc atagcttgcg
     6361 ttccgcacag atgatttctg ccgagcagac ttccttgccg tcgacgctgg ccttgcactc
     6421 gaacttccag atgctgcgct tgctgctcag gtagcgagcc tcaaggataa gctgatcacc
     6481 cgggactact ggctggcgaa agcgtagctt gtcggagcca acaaagtaat acagggtgcc
     6541 atcggagggt ttcaaatcca tcatcttgaa cccgagaatg ccggcggcct gagccatggc
     6601 ttcgatgatc aaaacgcccg gcataatcgg gtgctcagga aaatgcccgt tgaagaacgg
     6661 ctcattgatg ctgacattct tgtaggcgcg aattcgcttg ccttcaatat ccagctcagc
     6721 aacccgatcc accaaaagga acggatagcg gtgcggcaaa tattcgcgaa tctggttaat
     6781 gtccatcatg tgcagagaag cctgtaagag aaaaaagaga agccggtcaa taccggctca
     6841 ggcatcagat gaagtgtcgg cagctggggt cacggcggcc agctgctttt ccaactgttg
     6901 cagacgtcgc gccatatcat ccaactgacg aatccgagcg gcgctcttgc gccattcagc
     6961 cgctggctgc atggcggtgc ccgacgaata ggagccaggt tcggtgatcg agcgagtgac
     7021 catggtcata ccggtgacga acacgttgtc gcacacctcg atatgcccaa ccatgcccac
     7081 tccgcctgcg atcatgcagt tcttgccgat cttggtgctg cctgaaatac ccgcacaacc
     7141 ggccatggcc gtgttgtcgc cgatctgcac gttgtgcgcg atcatgatct ggttatccag
     7201 cttgacccca ttgccaatca gcgtatccga cagcgcgcca cggtcgacgg tggtattggc
     7261 gccaatctcg acatcatcac cgatggtcac cccaccgatc tgcgcgattt tttgccagac
     7321 gcccttctcg ttggcaaagc cgaagccctc gccgccgatc accgcgccag attgaataac
     7381 cacacgcttg ccgatgcgta cgtcgtgata cagggttacc cgcggagcca accagccgcc
     7441 ctcacctatc accgaacgcg caccgacaac actctgcgcg ccgacggtta cgccagcggc
     7501 aatctgcgcg ccactttcga taaccgcata agcgccgata caggcactcg tatcaacctg
     7561 cgcatccgcg gccaccacag ctgtgggatg cacgccaggc aatgcccgag gtttgcggtc
     7621 aaacagatgg gagagctcgg cataggccaa gtaaggatta gcaacgacca aggcattgcc
     7681 ggcatagccg tcggcatcag cggcggtcaa aagcaccgcg cccgcctggc agcccgagag
     7741 aaacttacgg tactgcgcat tggcgagaaa actcagttga tcagcgccag cctcctgcaa
     7801 ggtggccaga ccgctgatcg atttgtctgc ggtaccacgc aaggtggcac ccagacgctc
     7861 ggccaactga ccaagggtaa aaaccgttgc actcatgatc agcgcagctt gttcatacgc
     7921 tcgataactt gacgggtgat gtcgaactgc ggcttgacat caatcaccgc tccgcgctcc
     7981 agcaccaagt cgaagttacc agtcttgatc acttcttcca cggccttatc caggttcggc
     8041 ttgagcttct tcaacatttc acggtccgcc acagccttgg actcgttcag ctctttggac
     8101 tgaaactgga agtcacgggc tttttgcttg aattcaagct ccaggcgctc acgctcagcg
     8161 gtctgcattt tttcgccgtc tttaaccaga cgatcctgaa tgcgcttagc gtcgctttcc
     8221 agggttttca gcttgttcag ctgcgggccg aacttcttct cggcatcaac cgcataacgc
     8281 ttggctgcgt cagactccag caaagccatt tgatagttca gtaccgctac tttcatctcg
     8341 gcaaaagccg gagttgccag cagggcaacg cttaacaaaa ccagttgagt caacttacgc
     8401 acggtgcaac tcctgcaaaa ctgttggtat tcaaaaatct gacgtttaga acgtctgacc
     8461 gagagagaac tggaaaatct gcgtatcggc atcatccggc ttgatgattg gcatggccag
     8521 gctgaaactc aacggaccca gtgcggtaat ccaggtcagc cccacaccca cagagctagc
     8581 caagttaccc gggtcaatac tgccgcactc ttcctgggac ttgccgcagt tggtgtcgaa
     8641 gacattaccc acatcccaga acagcgcggt acgtagcgag cgctgatcct tgacgaatgg
     8701 catggggaac agcagttcca caccaccttg aatcaggacg ttaccaccga atggcagcgg
     8761 atcttgatcc ggatcctcca gcgtaccttc attaccagtt actttctcac caagactagg
     8821 cgtactgcgc ggaccaagac tgctgtcctt gaagcctcgt accgagttga aaccacccgc
     8881 atagtagttc tcatagaatg gcaggccttc tgtcgaacca taactatcgc catagcccaa
     8941 ctggctatgc agacgcaggg tataggtgtc gctcagcggt ttgaatatct ggccacggta
     9001 atcaaccttg aagaacgaca agtcacttcc cggcactgtg gtttcgaata ccaggctttg
     9061 cgaatgacca cggtttgcca gtacgccacg gttcagagtc gattcagacc aaccaaccga
     9121 cgctttaagg ttcagatagc tgtcaccttc agcttcgata aaatcgaaaa tttcatcgac
     9181 ggtatacacg ccagtgccga tgctgtcgtt ctggatggtc aggccataag tcagacgcga
     9241 agtatcactg attgggtaac cgatactggc acccacgccg tagctgtcca ccgagtagct
     9301 ggacacatcg gtattcagtt cgtcgtaatc ggtttcacgg aagaaggcgt tgtagcctag
     9361 gctgacgcca tctggcgtcc agtaggggtc aacaaaacca aagttgtagt tgctctggta
     9421 ttcgctgcgg gtcaggccga cgctgacctt gttaccggta cccaggaagt tgttctgggt
     9481 gatcgagccg ccgagaatca gaccggcgtt ctgagcgaaa ccgacgcttg cagtgataga
     9541 gccggaagcc tgctcttcaa cgctgtaatt gacgtcaacc tgatcgtcgg tgccaggcac
     9601 ctgtggggtt tcgacgttga cttccttgaa gtagccgaga cgctccagac gggtcttgga
     9661 ctgatcgatc aggtaagtcg atgcccagcc accttccatc tggcgcattt cacggcgcag
     9721 tacttcgtct tccgacttgg tgttgccacg gaaattgatg cgattcacgt aagcacgctt
     9781 gcctggatcg accacaaagg taatcgacac ggtgccgtca tcatgggctt ccggcacgcc
     9841 gttgacgttg gcgaaggtat agccttcgtt acccagacga cgggtgatca gctcggatgt
     9901 ggtggtcatc actttacgcg agaacacctg gccttcctga accagcagca aagcgcgaac
     9961 ttcttcttcc gggaccttca gttcaccgct gagcttgacc tcacgtacgg tgtacttctc
    10021 gccttcgtcg acgttgaccg tgacatagac gtgtttttta tccggggtaa tggatacctg
    10081 agtcgagctg atatccatat tgatatagcc gcgatccagg taataggagc gcagacgctc
    10141 caagtcgccg gagagcttct cacgggcgta cttgtcatca ttcttgaaga aggacagcca
    10201 gttggtggtc ttcagctcga acaggtcgat caggtcctca tcggaaaaga ccgagttacc
    10261 caccacgttg atgtgctgaa tcgcggcaac cgagccttca ttgatattga tcttcagcgc
    10321 cacgcggtta cgcggctgcg gaatcacttc ggcttcgatt tcggcggaat aacgaccctg
    10381 agcaacgtac tgacgctgca attcgttgcg tacgccttca agggttgccc gctggaagat
    10441 ctcgccttcg gccagacccg actgattcag gcccttgagc aggtcttcgc tgctgatggc
    10501 cttgttacca tcaatttcga tactggaaat cgacggacgc tcaaccacag tcacaaccaa
    10561 cacatcgcca tcacgaccga gctggatgtc ctggaaaaag ccggttttga acagttcgcg
    10621 agtagcctca accagtgatc ggtcatcagc ttctgcgccc acgttaaggg gcaaggcacc
    10681 gaatacgctg cccgcggaaa cgcgctgcag accggtgacc cggatatcgg agatggtgaa
    10741 ggactcggcg tgaacttcgg tgatcatcag tgcggcaagc accgcaggta gcagcagacg
    10801 tttcatgaag tcctttctta ttccaactga c
//
LOCUS       DASRUM010000172         7381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_253670, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000172 DASRUM010000000
VERSION     DASRUM010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7381
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253670"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="VFF22_11060"
     CDS             complement(<1..173)
                     /locus_tag="VFF22_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003184865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZX17391.1"
                     /translation="MTLENISCQKSFGGWHKRYRHRSQVLGCDMQFAVYLPPQAEQGA
                     KLPVLYWLSGLTCT"
     gene            complement(215..1327)
                     /locus_tag="VFF22_11065"
     CDS             complement(215..1327)
                     /locus_tag="VFF22_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HZX17392.1"
                     /translation="MIKSRAAVAFAPNQPLQIVEVDVAPPKAGEVLVRIVATGVCHTD
                     AYTLSGEDSEGVFPAILGHEGGGIVEAIGEGVTSVQVGDHVIPLYTAECRACKFCKSG
                     KTNLCSSVRATQGKGLMPDGTSRFSYNGEPIYHYMGCSTFSEYTVLPEVSLAVIPKDA
                     PLEKVCLLGCGVTTGIGAVLNTAKVEEGATVAIFGLGGIGLAAIIGAKMAKASRIIAI
                     DINPAKFEVARELGATDFVNPKDFAKPIQEVIVEMTDGGVDYSFECIGNVQLMRAALE
                     CCHKGWGESTIIGVAPAGAEISTRPFQLVTGRVWRGSAFGGVKGRTELPSYVEKAQKG
                     EIPLDTFITHNMPLEDINKAFDLMHEGKSIRTVIHF"
     gene            1453..2340
                     /locus_tag="VFF22_11070"
     CDS             1453..2340
                     /locus_tag="VFF22_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX17393.1"
                     /translation="MNRWEGLDEFVAVAECGQFTAAAERLGLSSSQVSRQIARLEERL
                     QTRLFYRSTRRVALTEAGQTFLQHCQRLQDAREEALRAVGDLGSEPKGLLRMTCAVAY
                     GERFIVPLVTDFMLQHSQLRVDIELSNRTLDLLHDGLDLAIRLGRLQDSRLVAMRLAP
                     RQMYLCAAPEYLQRYGRPHSLSELARHNCLIGSSDLWSFQANGRDSSLRVQGNWRCNS
                     GQAVLEAALRGLGLCQLPDYYVLEHLRSGALVSLLDNQQPPDTAVWALYPQQRHLSPK
                     VRQLVEFLKQGLAQRSEYR"
     gene            complement(2479..3189)
                     /gene="ispD"
                     /locus_tag="VFF22_11075"
     CDS             complement(2479..3189)
                     /gene="ispD"
                     /locus_tag="VFF22_11075"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZX17394.1"
                     /translation="MKTCELPPFWALIPAAGIGSRMRADRPKQYLQLAGKSIIEHTLA
                     CFLDHPQLRAVVVSLAVDDPYWPTLDCACDPRIQRADGGAERADSVLSGLLRLSELGA
                     QEQDWVLVHDAARPNLARSDLDLLLAELADDPVGGLLAVPARDTLKRAGADGRVQATI
                     DRSIIWQAFTPQMFRLGELQHALADALIASQAITDEASAIEWAGQAPRLIEGRADNLK
                     VTRPEDLRSLASLWHLKS"
     gene            complement(3186..3467)
                     /gene="ftsB"
                     /locus_tag="VFF22_11080"
     CDS             complement(3186..3467)
                     /gene="ftsB"
                     /locus_tag="VFF22_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HZX17395.1"
                     /translation="MRNNFYWVFIVLIAVLAGLQYRLWVGDGSLAQVAQLQQQIADQQ
                     GENQQLQERNRILEAEVLELKSGMETVEERARHELGMLKEGETLYQLAE"
     gene            complement(3533..4822)
                     /gene="eno"
                     /locus_tag="VFF22_11085"
     CDS             complement(3533..4822)
                     /gene="eno"
                     /locus_tag="VFF22_11085"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZX17396.1"
                     /translation="MAKIVDIKGREVLDSRGNPTVEADVILDGGIIGRACAPSGASTG
                     SREALELRDGDKSRYLGKGVLKAVANINGPIRDLLLGKDATDQKALDLAMIALDGTEN
                     KATLGANAILAVSLAAAKAAAQAKGVPLYAHIADLNGTPGVYSMPVPMMNIINGGEHA
                     DNNVDIQEFMVQPVGAKTFADALRMGTEIFHHLKAVLKARGLSTSVGDEGGFAPNLAS
                     NEDALAAIAEAVANAGYTLGDDVTLALDCASSEFFKDGKYDLAGEGKVFDAAGFADYL
                     AGLSERYPIISIEDGMDESDWAGWKVLTDKIGSKVQLVGDDLFVTNTKILKRGIDESI
                     GNSILIKFNQIGTLTETLEAIQMAKAAGYTAVISHRSGETEDSTIADLAVGTAAGQIK
                     TGSLCRSDRVSKYNQLLRIEEQLAGKAPYKGRAEFRG"
     gene            complement(4968..5813)
                     /gene="kdsA"
                     /locus_tag="VFF22_11090"
     CDS             complement(4968..5813)
                     /gene="kdsA"
                     /locus_tag="VFF22_11090"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HZX17397.1"
                     /translation="MTQKIIKVGNIEIANDKPFVLFGGINVIESRDLAMRACEEYVRV
                     TEKLGIPYVFKASFDKANRSSVTSFRGPGLEEGMKVFEEIKKTFGVPVITDVHEPEQA
                     APVAAVCDIIQLPAFLSRQTDLVVAMAKTGAVINIKKAQFLAPQEMKHILSKCEEAGN
                     DQLILCERGSSFGYNNLVVDMLGFGIMKQMNYPVFFDVTHALQMPGGRADSAGGRRAQ
                     VTDLAKAGMSQGLAGLFLEAHPDPDNAKCDGPCALRLDKLEPFLTQLKQLDDLIKSFA
                     PIETA"
     gene            complement(5818..7380)
                     /locus_tag="VFF22_11095"
     CDS             complement(5818..7380)
                     /locus_tag="VFF22_11095"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017906258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZX17398.1"
                     /translation="MAAILEARGLKVTMLKLDPYINVDPGTMSPFQHGEVFVTHDGAE
                     TDLDLGHYERFIRTTMTKSNNFTTGRVYEDVLRRERRGDYLGATIQVIPHITDEIKRR
                     IIKGAGDADVAMVEIGGTVGDIESQPFLEAIRQLRVEVGAKRAMLMHLTLVPYIATAG
                     ETKTKPTQHSVKELRSIGLQPDVLICRSDHPIDISSRRKIALFTNVEERAVISLEDVD
                     TIYKIPGVLHAQGLDDFVVERFGLECNGADLSEWDRVVDAKLNPEHEVTIAMVGKYME
                     LLDAYKSLIEAMSHAGIQNRTKVNLRYIDSEDIENKGTALLEGVDAILVPGGFGLRGV
                     EGKIKTVQYARENKIPYLGICLGMQVAVIEFARNVLGWSDANSTEFDTVSTHPVVGLI
                     TEWQDATGVTEQRTDASDLGGTMRLGAQDCQLLDGSQVHQCYGKDVIVERHRHRYEVN
                     NNLLPQLIEAGLKVTGRSGDGALVEVVEAPDHPWFVACQFHPEFTSTPRDGHPLFSGF
                     VSAALAQHAKKA"
BASE COUNT         1452 a         2221 c         2202 g         1506 t
ORIGIN      
        1 gtgcaggtca ggccgctgag ccagtacagc accggcagct tcgcgccctg ctcggcctgc
       61 ggcggcaggt acacggcaaa ctgcatatcg cagcccagca cctgggagcg atgccggtaa
      121 cgcttgtgcc agccgccaaa gcttttctgg caggaaatat tctccagagt catgacgtcc
      181 ccgtagggtg cgctgtgcgc accacaactg tgtattagaa atgaatgacg gtacggatgc
      241 tcttgccttc atgcatcagg tcgaacgcct tgttgatgtc ttccagcggc atgttgtggg
      301 tgataaaggt gtccaggggg atctcaccct tctgcgcctt ttccacatag ctcggcagct
      361 cggtacggcc tttgacgcca ccgaaggccg aaccgcgcca gacgcgcccg gtcaccagct
      421 ggaatggacg ggtgctgatc tccgcaccgg ccggtgccac accgatgatg gtcgactcgc
      481 cccagccctt gtgacagcac tccagcgccg cacgcatcag ttgcacatta ccgatgcact
      541 caaagctgta gtccacgccg ccgtcggtca tctcgacgat aacttcctga attggcttgg
      601 cgaaatcttt cgggttgacg aagtcagtcg cacccagctc acgggccacc tcgaacttgg
      661 ctggattgat atcgatggcg atgatgcgcg aggccttggc catcttcgcg ccgatgattg
      721 ccgcgagacc aataccgccg aggccgaaga tcgccacggt ggcgccctct tccacttttg
      781 cggtgttgag cacagcgcca atcccggtgg tcacgccgca gccaagcagg cagactttct
      841 ccagcggcgc gtccttgggg atcaccgcca gggagacttc cggcagcacg gtgtattcgg
      901 agaaggtcga gcagcccatg tagtggtaga tcggctcgcc gttgtaactg aagcggctgg
      961 tgccgtcggg catcaggccc ttgccctggg tcgcccgcac cgagctacac aggttggttt
     1021 tgccggattt gcagaatttg caggcgcgac attcagcggt gtacagcgga ataacgtggt
     1081 cgccgacctg caccgaggtc acgccctcgc caatagcttc gacgatgccg ccgccttcat
     1141 ggccgagaat cgccgggaac accccttcgg aatcctcacc ggacaaggtg taggcatcgg
     1201 tgtggcacac gccggtggcg acaatgcgca ccagcacttc cccagccttg ggcggcgcca
     1261 cgtcgacttc gacgatttgc agcggctgat tgggggcaaa ggctacggct gcacgggact
     1321 tgatcatgat ctctctccag cggactcaac ggtggcgcag agtgtagatc agtgattaag
     1381 atagataatc cgccaaaaaa caaaacattc ttgctatata gggataatca aaccgtacca
     1441 aggatatcca ccatgaatcg ctgggaaggc ctcgatgaat ttgtcgccgt ggccgaatgc
     1501 ggccaattca ctgcggccgc cgagcgcctg ggactgtcgt catcccaggt cagccggcag
     1561 atcgcgcgcc tggaagaacg cctgcagacc cggttgttct atcgcagcac ccgccgtgtg
     1621 gcgctgaccg aggccgggca gacgttcttg cagcattgcc agcgcctgca ggatgcccgc
     1681 gaggaagccc tgcgcgcggt gggcgatctg ggcagcgaac ccaaaggcct gctgcgcatg
     1741 acctgcgcgg tggcctacgg cgagcgcttt atcgtgccgc tggtcacgga ctttatgctg
     1801 cagcattcgc aattacgggt ggatatcgag ctaagcaacc gcacccttga tttgctgcac
     1861 gacggcctcg acctggccat tcgcctgggt cgcctgcagg actcgcggct ggtggctatg
     1921 cgcctggcac cgcggcagat gtacctgtgc gcagcccccg aatacctgca gcgctacggg
     1981 cgcccgcact cgctgtcgga gctggcgcga cacaactgcc tgatcggcag ttcggatctc
     2041 tggagttttc aggccaatgg ccgcgattcc agcctgcggg tacaaggcaa ctggcgctgc
     2101 aacagtggcc aggcggtgct ggaagcggcg ctgcgcggtc ttggcctatg tcagttaccg
     2161 gattactacg tgctggagca tctgcgcagc ggcgcactgg tctccctgct ggacaaccag
     2221 caaccgccgg ataccgccgt atgggcgttg tatccacagc agcggcatct gtcacccaag
     2281 gtgcggcagc tggtggagtt tcttaaacag gggttggcgc agcgcagcga gtatcgctag
     2341 aaccagtttt cgatcttgcg agctagagcc ctgcaaggca aaaacagggg aggaagcgga
     2401 gtgtactttt gtacatgagc attctgagcc tgtttttaac gcggcagggc cgacgcgcag
     2461 cagatcgtaa ataggttctt agcttttcag atgccagagg ctggccaggc tacgcaggtc
     2521 ttcagggcga gtgaccttca ggttatccgc acgcccctcg atcaggcgcg gcgcttgccc
     2581 ggcccactcg atggccgagg cttcatcggt aatcgcctga ctggcaatca gagcatcggc
     2641 tagcgcgtgc tgtaactcac ccaaacggaa catctgcgga gtaaaggcct gccagatgat
     2701 cgagcgatct atggtggcct gcacccggcc gtcggcgccg gcgcgcttga gcgtatcgcg
     2761 ggcgggcacg gcgagcaggc cgcctactgg atcgtcagct agttcggcga gcaacagatc
     2821 cagatcactg cgtgccagat tgggccgcgc cgcatcgtgc accaacaccc agtcctgctc
     2881 ctgagcaccc agctcgctga ggcgcagcaa accgctgagt accgagtcgg cacgctcggc
     2941 accgccatcg gcgcgctgaa tacgtggatc acaggcacag tccagagtcg gccagtacgg
     3001 atcgtccacc gccaggctga ccactaccgc gcgcaattgc ggatgatcga gaaagcacgc
     3061 cagggtgtgt tcgataatgc ttttgccggc cagttgcagg tactgcttgg gccggtcggc
     3121 gcgcatccgg ctgccaatgc cggcagccgg gatcaacgcc cagaaagggg gtaattcgca
     3181 ggttttcatt cagccaactg atagagggtt tcgccctctt taagcatgcc cagctcatgc
     3241 cgcgcacgct cttcgacggt ttccataccg cttttcagct ccagtacttc ggcttcgaga
     3301 atccggttgc gctcttgcag ctgctggttt tcgccctgtt gatcggcgat ctgctgctgc
     3361 aactgggcca cctgcgccag gctgccatca ccgacccaca ggcgatattg caaacctgcc
     3421 aacacggcaa tcagcacgat aaatacccag taaaaattat tgcgcatagc gtaaaaagct
     3481 tccgaacgaa aaaggggcga ctcgcgccgc ccctctttta ccatgcctgg gttcaaccac
     3541 ggaactcggc gcggcctttg tatggtgctt taccagccag ctgctcttcg atacgcagca
     3601 attggttgta cttggacacg cggtccgagc ggcacagcga gccggtcttg atctggccag
     3661 cagcagtacc cacagccaga tcggcgatgg tgctgtcttc ggtttcaccc gagcggtgcg
     3721 agatcaccgc ggtgtaaccc gcagccttgg ccatctggat ggcttccagg gtctcggtca
     3781 gagtgccgat ctggttgaac ttgatcagga tcgagttgcc gatcgactcg tcgatgccgc
     3841 gcttgaggat cttggtgttg gtcacaaaca gatcgtcacc gaccagctgt accttgctgc
     3901 cgatcttgtc ggtgaggact ttccagccag cccagtcgga ctcgtccatg ccgtcttcga
     3961 tggagatgat cggataacgc tcggacaggc cagccagata gtcggcgaag ccggcagcgt
     4021 caaacacttt gccttcgcca gccagatcgt atttgccgtc cttgaagaac tcgctggaag
     4081 cgcagtccag cgccagggtg acgtcgtcgc ccagggtgta accggcattg gccacagctt
     4141 cagcgatggc agccagagcg tcttcgttgg aggccaaatt cggcgcaaag ccgccttcgt
     4201 cacccacgga ggtgctcagg ccacgggcct tcagcacggc tttgaggtga tggaaaatct
     4261 cggtgcccat gcgcagcgcg tcagcgaatg ttttcgcgcc caccggctga accatgaatt
     4321 cctgaatatc gacgttgtta tcggcgtgct cgccgccgtt gatgatgttc atcatcggca
     4381 ccggcatgga gtaaacgcct ggagtgccgt tcagatcggc gatatgcgcg tacaacggca
     4441 cacccttggc ctgagcggca gctttggcgg cggccaggga cacggcgagg atggcgttgg
     4501 cacccagggt ggctttgttt tcagtaccgt cgagggcaat catcgccagg tcgagggctt
     4561 tctggtcagt ggcatctttg cccagcagca ggtcgcggat cgggccattg atgttggcca
     4621 cggccttcag cacgccctta cccaggtaac ggctcttgtc gccatcacgc aattccagcg
     4681 cttcacgcga accagtggag gcaccggacg gcgcgcaggc gcgaccgatg atgccaccgt
     4741 cgagaattac atcggcttcc acagtcgggt taccacggga atcgagaacc tcacggcctt
     4801 tgatgtcgac gatctttgcc attgcgtgta gcactccaaa aaattgacga aaagggtagt
     4861 accgagctgc acactcattg cacgccgaag gttaacccag cgcacaacat gtaccgacta
     4921 tttaatcaga ttgtatacaa gtggtacttt accggagatt tgccgtttca ggcagtctca
     4981 atcggcgcaa aactcttgat caaatcatcc agctgcttga gctgggtgag gaacggctcc
     5041 agtttatcca gacgcagggc acaagggccg tcgcatttag cgttgtccgg gtccggatgg
     5101 gcctcgagga acaagcctgc caggccctga ctcatgcctg ccttggccag atcagtcacc
     5161 tgggcacggc gaccaccggc ggaatcggcg cgcccacccg gcatttgcag ggcgtgggtc
     5221 acgtcgaaga acaccggata gttcatctgc ttcatgatgc cgaagccaag catgtccact
     5281 accaggttgt tgtaaccgaa gctggaaccg cgctcgcaga gaatcagctg gtcgttgccc
     5341 gcctcttcgc acttggacag gatgtgtttc atctcctggg gcgcgaggaa ctgggccttc
     5401 ttgatattga tcaccgcgcc ggtcttggcc atggccacta ccaggtcggt ctgccgcgaa
     5461 agaaacgccg gcagctggat aatgtcgcag accgcagcta ctggagccgc ttgctcaggc
     5521 tcatgcacgt cagtgatcac cggcacgccg aaggtcttct tgatctcttc gaagaccttc
     5581 atgccctctt ccaggcctgg gccgcggaac gaggtcaccg aagaacggtt ggccttgtcg
     5641 aaactggcct tgaatacata gggaatgccg agcttctcgg taacccgcac gtactcttcg
     5701 caggcgcgca tagccagatc gcgggactcg ataacattga tgccgccgaa cagcacgaat
     5761 ggcttgtcgt tggcgatctc gatgttgccg accttgataa ttttctgggt catatcctta
     5821 tgccttcttc gcgtgttgcg ccagggcggc gctaacgaaa ccgctgaaca atgggtgacc
     5881 gtcgcgcggc gtcgaggtga actcggggtg gaactggcag gccacaaacc aaggatgatc
     5941 cggtgcctcc accacttcaa ccagcgcgcc gtcgccggag cggccggtga ccttgaggcc
     6001 ggcttcgatc agctgcggca acaggttgtt gttcacttca taacgatgac gatggcgttc
     6061 aacgatcaca tccttgccgt agcactgatg cacttgcgag ccgtccagca actggcagtc
     6121 ctgggcaccg aggcgcatgg tgccgcccag atcggaagcg tctgtacgct gttcggtcac
     6181 gccggttgcg tcctgccatt cggtgatcag accaaccact ggatgagtgc tgacggtgtc
     6241 gaactcggtg gagttggcat cgctccagcc cagcacattg cgggcgaact cgataactgc
     6301 gacctgcatg ccgaggcaga tacccaagta gggaatcttg ttctcacggg cgtactgcac
     6361 ggtcttgatc ttgccttcca cgccacgcag accgaagccg cccggcacca gaatggcgtc
     6421 cacaccttca agcagcgcag tgcctttgtt ctcgatatct tcggagtcga tataacgcag
     6481 gttgaccttg gtgcgattct ggatgcctgc atggctcatc gcttcgatca gcgatttgta
     6541 ggcatccagc agctccatgt acttgccgac catggcgatg gtgacttcgt gctctggatt
     6601 cagcttggca tcgacgacac ggtcccactc ggacagatca gcaccgttgc attccaggcc
     6661 gaagcgctcg acgacgaaat catccagacc ctgggcgtgc agcacgcctg ggatcttgta
     6721 gatggtgtcg acgtcttcca gggaaatcac cgcacgctct tcaacgttgg tgaacagcgc
     6781 gattttgcgc cgcgaggaaa tatctatcgg atggtcggaa cggcagatca gcacatccgg
     6841 ctgcagaccg atggaacgca gctctttcac cgaatgctga gttggcttgg tcttggtttc
     6901 gccagcggtg gcgatatacg gcactagggt caggtgcatc agcatggcgc gcttggcgcc
     6961 tacttccaca cgcaactggc ggatggcttc gaggaacggt tgcgactcga tgtcgccgac
     7021 cgtgccgccg atttcaacca tggctacgtc agcgtcgcca gcacccttga tgatgcggcg
     7081 cttgatctcg tcggtgatgt gcgggatgac ctgaatggtc gcgcccaggt aatcaccacg
     7141 gcgctcgcgg cgcagtacgt cttcgtagac gcggccggtg gtgaagttgt tgctctttgt
     7201 catggtggtg cggataaacc gctcatagtg gcccaggtcg aggtcggtct cagcgccgtc
     7261 gtgggtgacg aacacctcac cgtgctggaa cgggctcatg gtgcccggat cgacgttgat
     7321 atagggatcc agcttgagca tggtgacctt caggccccgc gcctccagga tagccgccaa
     7381 t
//
LOCUS       DASRUM010000173         9702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2258617, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000173 DASRUM010000000
VERSION     DASRUM010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9702
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2258617"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1396)
                     /locus_tag="VFF22_11100"
     CDS             complement(77..1396)
                     /locus_tag="VFF22_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HZX17399.1"
                     /translation="MNAVVAAVGIMLILSLCRVHVVVALIIGALAGGLLGGLGVEGSL
                     AAFNKGLGGGATVALSYALLGAFAVAIAKSGLAHALADKALAMVGKQDAKGAGLLKWL
                     LIALLLAVAISSQNILPIHIAFIPLLVPPLLYVLGKLQLDRRLIACVLTFGLITPYMF
                     LPVGFGGIFLNDILLANVASGGVDTSGLDVTKAMAIPALGMLFGLLVAVLFSYRKKRV
                     YDLSKIEQAERVDVAYNPLSLLVAGVAIVAAFVVQLWLDSMIIGALVGFIIFSVSGVV
                     RWKEADGLFTEGMKMMAMIGFIMIAAAGFAEVMKATGEVKSLVDSSAELIGHDKALGA
                     LLMLLVGLLVTMGIGSSFSTVPIIAAIFVPLGLQLGFSPLAILCIVGTAGALGDAGSP
                     ASDSTLGPTAGLNVDGQHSHIWDSVVPTFLHYNLPLLAFGWAAAMVI"
     gene            1674..3818
                     /locus_tag="VFF22_11105"
     CDS             1674..3818
                     /locus_tag="VFF22_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX17400.1"
                     /translation="MLFSRLSIQLKITLLAGLCLLAIVAVLVSASVIQASRSASLVKQ
                     ASSAMLQESAQLRMSARGESQALQMQRYFMDAYQYGRGAATQVLFIREQAEKRFLDAF
                     DLREDLTRQVRSALEANRSLLGIYVVFEPNALDGKDELFTDQENLGSNDKGRFSIYWA
                     QGDNGELSAEAMKEADLNDATPGISGAPYNAWYSCPLQQKKACLLDPYIDDADGVKTL
                     MTSIAFPLEQGGKVIGVLGVDISLASLQELSTSAHQGLFDGAGHVSIISPAGLLAGHS
                     RDSSLLGGKLEKAFADQHREMTSLVASNRPEVLHHDGALQVLQPLLPIPESQPWGVLM
                     EVPESVLLEPATRLETQMDEMGTRSTAVSLSLGLAAGVVGILLIWLTALGVTRPILGV
                     AAMLKNIASGEGDLTRRLEYAKQDELGELASWFNRFLDKLQPIIADVKRSVQDARSTA
                     DQSALIASQTSDGMQQQFREVDQVATAFQEMSATAQDVAHNAAQAAEAARTADQASRE
                     GMSVIGKTTSTIELLANEMNVAMQEVEGLANSSEKIGSVLEVIRSIAEQTNLLALNAA
                     IEAARAGEAGRGFAVVADEVRNLAKRTQDSVEEIRQVIEGLQSGTKEVVSTMHSSHRQ
                     AQGSVEQVEQAVAVLRRISDAVSVITDMNLQIASAAEEQSSVAEEINRNVASIRDVTE
                     SISAQADESAKISQGLNKLANHQQSLMDQFRV"
     gene            3929..4525
                     /locus_tag="VFF22_11110"
     CDS             3929..4525
                     /locus_tag="VFF22_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZX17401.1"
                     /translation="MLNIALVAGSGRNNSQSGKVARFLRQRLIQLGHTDDAQSNVIDL
                     GLAPLPLWPAEDAGPWGLYSKQLAAADAVVIISPEWNGMACPAIKNFFIYASKAELAH
                     KPGLLVGISSGVGGAYPISELRASSYKNCRLCYLPEHLIVRSVEKVLNTPEPSSEDDQ
                     RLHARIDYELDILVKYAQALGPVRGSIDMSNPAFANGM"
     gene            complement(4553..5872)
                     /locus_tag="VFF22_11115"
     CDS             complement(4553..5872)
                     /locus_tag="VFF22_11115"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008021677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZX17402.1"
                     /translation="MRSIQRRLSLGLISVLLVIGLLLAQSSLWLFDRSLRSYLQTTLQ
                     DETQTLLGAVVRGPSGLQLDERRLSAAFKRPYSGLYFRIDLGEQTWRSRSLWDRSLRP
                     VEETGLQDALSDGPQGQQWLVYRADYQRYGQPIVIHVARDYTPILNSFSQVQKIVLGL
                     GITALVLILLLQRLIVSRALRPLEQTRRQLMQLQQGQRSQLDQQVPLELEPLVAQINH
                     LLAHTEDILKRSRNALGNLGHALKTPLAVLVSLATREELKQLPQLRESLNGQLQQIEL
                     RIARELGRARLAGEALPGAHFDCANELPELFSTLAMIHGNHLELDWQAEPGLRLPWDR
                     EDLLELLGNLLDNACKWADSQVQLQVSKVGESYQLTIDDDGPGIDAAQRDEVLSRGSR
                     LDEQVAGHGLGLGIVRDIIDAWAGRLQLADSPLGGLRVTVTLPVKRT"
     gene            complement(5869..6537)
                     /locus_tag="VFF22_11120"
     CDS             complement(5869..6537)
                     /locus_tag="VFF22_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZX17403.1"
                     /translation="MRLLLVEDHVPLADELLAGLTRQGYAVDWLADGRDAAYQGATEP
                     YDLIILDLGLPGKPGLEVLQEWRTGGLTTPVLILTARGSWAERIEGLKAGADDYLSKP
                     FHPEELQLRIQSLLRRAHGLANQPQLEAAGLQLDEGRQTVKRNDEEVQLTAAEFRLLR
                     YFMLHAGQLLSKSHLAEHLYDGESERDSNVIEVHVNHLRRKLGREIIETRRGQGYRFS
                     GDAV"
     gene            complement(6538..6855)
                     /locus_tag="VFF22_11125"
     CDS             complement(6538..6855)
                     /locus_tag="VFF22_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HZX17404.1"
                     /translation="MNSTAWLARAFLACLLLTLTFSSSARDLDQDEALRLRREGLIMP
                     LEQLLQMALKPHPGAVLLEVELEEEDGVLVYEVELVTEQGIVRELELHASTGEVLKDE
                     VED"
     gene            complement(6855..7163)
                     /locus_tag="VFF22_11130"
     CDS             complement(6855..7163)
                     /locus_tag="VFF22_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HZX17405.1"
                     /translation="MKTLTAVFAAAALTLTAGLAQARDLGPDEALKLRDAGTIQSFEK
                     LNAAALALHPGSKTEDTELEEEYGRYIYQVEVRDAQGVQWDVELDATNAQVLKNQRDD
                     "
     gene            7359..8438
                     /locus_tag="VFF22_11135"
     CDS             7359..8438
                     /locus_tag="VFF22_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZX17406.1"
                     /translation="MSAILIKSPALTIKAGKRALARIREHGLQPADVGILPGAAGGPK
                     ALGIQGLDLALFGDWLQRATRERSLIGASIGSWRFASACLPDAAAGLRRLGELYTSQR
                     FAKGVSMAQVSSSCSLMLDQLLGNDAANILSNPHYRLNIVVVKSHGLLQHDHRGALGL
                     GLSSVIGSNLLGRPRLAKHFERVILHDARLAPPLGALSDFPSRYLQLDGGNLRHALLA
                     SGSIPMVMQGVRDIPGAGPGTYRDGGLLDYHLDLPYSGEDIVLYPHFTDKVIPGWFDK
                     GMPWRRGDAGRLQDVVLLAPSRDYLARLPHGKLPDRKDFSRYLGDDAGRERYWRTAMS
                     ESQRLGDEFLELVDSGRLGERLQAL"
     gene            8492..9331
                     /locus_tag="VFF22_11140"
     CDS             8492..9331
                     /locus_tag="VFF22_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17407.1"
                     /translation="MKLAVAIIHGIGTQPDLRNDEGQHDFAQNLIAGLRQRLGVEANQ
                     VAFQTLHWASVLDKRQLVFLDQLRDQPVRWRWLRRIVTMFFGDASGYRKVSEAYDTTY
                     EEVHQCLRSGLNALRARVDADTPLVVLAHSLGGHIFSNFVWDQQRINKAESCPLDPFL
                     ALETLSGLITFGCNIPLFTFAYDTVVPIRFPGHCLTEAVTEQARWLNLYAPADPLGYP
                     LRPLPSYAAVVAEDRAMPVGPWYKRHTPLSHLGYWDDRGFHRYVAGYLRQLLAVCKDR
                     ESV"
     gene            9521..>9702
                     /locus_tag="VFF22_11145"
     CDS             9521..>9702
                     /locus_tag="VFF22_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17408.1"
                     /translation="MNAPVRFNEALLIADRAFQPFQCVAWAPQDGTGNLSLSVIDRTS
                     TRLLGRTQLSSTIYSDP"
BASE COUNT         1990 a         3041 c         2811 g         1860 t
ORIGIN      
        1 ctaccgattt atcggtagcc ccgctgaaag gttgaatcgc ctggcgctgt agcgcgtcag
       61 gcgaacaatg ctccggttaa atcaccatag ctgctgccca accgaatgcc agcaacggta
      121 agttgtagtg caggaaggtc ggcaccacgc tgtcccagat gtggctgtgc tggccatcga
      181 cgttcagccc agcagttggg ccgagggtcg aatcggaggc tggcgagccg gcatcaccca
      241 gtgcgccagc agtgccgacg atgcacagga tggccagcgg actgaagccg agctgcaggc
      301 ccagtggcac gaaaatcgcc gcaatgattg gcacagtgga gaacgacgaa ccgatgccca
      361 tggtcaccag cagaccaacc agcagcatca gcagcgcgcc cagcgccttg tcatggccga
      421 tcagctcggc ggagctgtcg accaggcttt tcacttcgcc ggtggccttc atcacctcag
      481 caaaaccagc ggcggcgatc atgataaagc cgatcatagc catcattttc atgccttcgg
      541 taaacaggcc atcggcctct ttccagcgca ccacgccaga caccgagaaa atgataaagc
      601 cgaccagggc accgatgatc atcgagtcca gccatagctg cacgacaaac gccgcgacaa
      661 tcgccacgcc cgccaccagc aggctcagtg ggttgtaggc aacatccacg cgctcggcct
      721 gctcgatctt gctcaggtca tacacgcgct ttttgcggta gctgaacagc accgccacca
      781 gcaagccgaa cagcatgccc agcgccggaa ttgccatggc tttggtcacg tccaggccgc
      841 tggtgtcgac gccgccactg gcaacattgg ccagcagaat gtcattgagg aaaatcccgc
      901 caaagcccac cggcagaaac atataggggg taatcaggcc gaaggtcagc acgcaggcga
      961 tcaggcggcg gtcgagttgc agcttgccca atacatacag cagaggcggc accagcagcg
     1021 ggataaaggc gatatgaatt ggtaggatgt tttgcgagga aatcgccacc gccaacagca
     1081 gcgcaatcag cagccacttg agcagaccgg cgcctttggc gtcctgcttg ccgaccatag
     1141 ccagggcttt atctgccaga gcatgtgcca ggccggattt ggcaatcgcc acagcaaagg
     1201 cccctagcaa ggcataggac aacgccactg tcgccccacc acccaggcct ttgttgaagg
     1261 cggccagcga gccttcaaca cccaggccac cgagcagacc accggccaag gcaccgatta
     1321 tcagggctac taccacatgc acacggcaca agctgagaat cagcatgatc cccactgccg
     1381 caacgactgc gttcatcgat ttactcccgc cattaagtaa gtctgcgaag gttttgacct
     1441 tctctggatg ctgcgccggt tcggcgaagg gcgcgtactc tgcacaatgc tgactgacct
     1501 gtcaaaacac ccgtaaatta ttttgtacaa gctgtagcta atttcgatta ccctgcgact
     1561 ttaaagcccg ctactggcct ttcatttgct ctgctttact taacgtgaca cttaaagaaa
     1621 agcgcctaaa cgccgatagg cgctctaagc ctctgccata aaaaggtctg aaaatgctgt
     1681 tcagccgact ttccatccag ttgaagatca ccctactggc aggcctgtgc ttgctggcca
     1741 ttgttgccgt gctggtttcg gcctcagtga ttcaggcctc gcgcagcgcc agcctggtca
     1801 agcaggccag ctcggcaatg ctgcaggagt ccgcgcaact gcgcatgtcc gcgcgcggcg
     1861 agtctcaggc cctgcagatg cagcgctact ttatggatgc ctaccaatac gggcgtggcg
     1921 cagccaccca ggtgctgttt atccgtgagc aggcggaaaa gcgttttctc gatgctttcg
     1981 atctgcgcga agacctgacc cgccaggtgc gcagcgcact ggaagctaac cgcagcctgc
     2041 tggggattta tgtggtgttc gagcctaatg ccctggacgg caaggacgag ctatttaccg
     2101 atcaggaaaa cctcggcagc aacgacaagg gccgcttctc gatttattgg gcacaaggcg
     2161 acaacggtga actcagcgcc gaagcgatga aagaggcgga cttgaacgac gccacgcccg
     2221 gtatcagcgg tgcgccctat aacgcctggt acagctgccc actgcagcag aagaaagcgt
     2281 gcctgctcga cccctatatc gatgatgctg acggggtaaa aaccttgatg accagtatcg
     2341 cctttccgct ggaacaaggc ggcaaggtga ttggcgtact cggcgtggat atcagcctgg
     2401 ccagcctgca agagctcagc accagcgcgc accagggcct gttcgacggt gcagggcatg
     2461 tcagcatcat cagtcccgcg ggcttgctcg caggccacag ccgcgatagc agcctgctgg
     2521 gcggcaaact ggaaaaagcc ttcgccgacc aacaccgcga aatgaccagc ctggtcgcca
     2581 gcaaccgccc ggaagtcctg caccacgacg gtgccttgca ggtgttgcaa ccgctgttgc
     2641 ctattcccga atcccagccc tggggcgtat tgatggaggt gccagaaagc gtcctgctgg
     2701 agcctgccac ccgcctggaa acgcaaatgg atgaaatggg cacccgcagc acagcggtgt
     2761 ccttaagcct cggcctggct gccggggtag tgggtattct gctgatctgg ctcacggcgc
     2821 tgggcgtgac ccggccgatt cttggggtcg cggcgatgct gaaaaatatc gccagcggcg
     2881 aaggcgacct gacccgccgc ctggagtatg ccaagcagga tgaactgggc gaactggcca
     2941 gctggtttaa tcgcttcctc gacaagctgc aaccgatcat cgcggacgtg aaacgtagcg
     3001 tgcaggacgc ccgcagcacg gccgaccaat ccgcgctgat cgccagccag accagcgatg
     3061 gcatgcaaca gcagttccgt gaagtcgatc aggtggcaac cgccttccag gaaatgagcg
     3121 ccacggcgca ggatgttgca cataacgccg ctcaggctgc agaagcggcg cgcaccgccg
     3181 accaggccag ccgcgaaggg atgagcgtga tcggcaagac cacctcaacc atcgaactgc
     3241 tggccaatga aatgaacgtg gccatgcagg aagtcgaagg cctggccaat agcagcgaga
     3301 agatcggttc ggtactggaa gtgatccgtt caattgccga acagaccaac ctgctcgctc
     3361 tcaacgcagc gatcgaagcg gcccgcgccg gggaagccgg gcgtggcttt gccgtggtcg
     3421 ccgatgaagt gcgcaacctg gccaagcgca cccaggattc ggtagaggag attcgccagg
     3481 taatcgaagg cctgcaaagc ggcactaagg aagtggtcag caccatgcac agcagccatc
     3541 gtcaggccca gggcagcgtc gagcaagtcg agcaggccgt ggccgtactt cgccgcatca
     3601 gcgatgcggt cagcgtgatt accgatatga acctgcagat cgccagcgcc gctgaagagc
     3661 aaagctcggt agccgaggag atcaaccgca acgtggcctc gattcgggat gtcaccgagt
     3721 cgatttccgc ccaggccgat gaatcggcaa aaatcagcca gggcctgaac aaactggcca
     3781 accaccagca gagcctgatg gaccagttcc gcgtctagcc ggcatggcac catcggaccg
     3841 ccactctggc ggctgcttcc ttgtgacgca ggctaacaat cgttagcctg cgtttttcat
     3901 ttatgaccca ttaatcaaga ggtgcacgat gctcaatatc gccttggtgg ccggctctgg
     3961 ccgcaataac agccaatcag gcaaggtcgc ccgcttcttg cgccagcgcc tgattcagtt
     4021 gggtcacacc gacgatgcgc aaagtaacgt gattgacctg ggcctcgccc cgctgccgct
     4081 ctggcctgcc gaagatgccg gcccctgggg gctgtacagc aaacaattgg cggccgccga
     4141 tgccgtggtg atcatctccc cggagtggaa tggcatggcc tgcccggcga tcaagaactt
     4201 ctttatctac gccagcaagg ccgagctggc gcacaagccg gggctgctgg tgggcatatc
     4261 gtcgggagta ggcggggctt atccgatcag cgagttgcgt gcttccagct acaagaactg
     4321 ccgcctctgc tatctgcctg aacatctgat cgtgcgcagc gtcgagaagg tgctgaacac
     4381 gccagagcca agcagcgagg acgaccagcg cctgcatgca cgcatcgatt acgagctgga
     4441 tattctggtc aagtacgcgc aggctttggg ccccgtgcgc ggcagtatcg atatgagcaa
     4501 cccggcattt gccaacggca tgtaagcctt acgttaagcg tacctgatga actcaggtgc
     4561 gcttcaccgg caaggtaacc gttacgcgca agccacctag tggactgtct gccagctgta
     4621 ggcgccctgc ccaggcgtcg atgatatcgc gcacgatacc cagacccagc ccatgcccgg
     4681 ccacctgctc atccaggcgg ctgccacggc taagcacttc gtcgcgctga gcagcgtcga
     4741 tgcccggccc atcgtcatcg atggttaatt gatagctctc tcccaccttg ctgacctgca
     4801 gttgcacctg gctgtcggcc catttgcagg cgttgtccag caggttgccg agcagctcta
     4861 gcaggtcttc acggtcccag ggcaagcgca gcccaggctc ggcctgccaa tccagctcca
     4921 ggtggttgcc atggatcatc gccagggtcg agaacagctc aggcagctca ttggcgcagt
     4981 cgaaatgcgc cccaggcaag gcttcgccgg ccagccgggc acggcccagt tcacgggcga
     5041 tgcgcagctc gatctgttgc agctgtccat tgaggctttc gcgcagttgc ggcaactgct
     5101 tgagctcttc ccgggtggcc aggctgacga gcaccgccag cggcgttttc agcgcatggc
     5161 cgaggttgcc cagggcgttg cgtgagcgtt tgaggatgtc ttcagtgtgc gccagcaagt
     5221 ggttgatctg cgccaccaac ggttcgagtt ccagcggcac ttgttggtcc agttgactgc
     5281 gctggccctg ctgcagctgc atcagctgcc gacgtgtctg ttccagcggg cgcaacgcgc
     5341 ggctgacgat cagccgttgc agcaacagaa tcagcaccag ggcggtaatc cccaggccca
     5401 ggacaatctt ctgcacctgg ctgaagctgt tgagtatcgg cgtgtaatcg cgtgccacgt
     5461 gaatcacgat gggctggcca taacgctgat agtcggcgcg gtacaccagc cattgctggc
     5521 cttgtggccc gtcgctcagc gcgtcctgta aaccggtttc ttcaaccgga cgcaggctgc
     5581 gatcccagag cgagcgcgaa cgccaggtct gctcgcccag atcgattcga aaatacaggc
     5641 cggaatacgg ccgcttgaac gccgcgctga ggcgccgctc atccagttgc aggccgctgg
     5701 gaccacgcac cactgcaccc agcaaggtct gcgtttcatc ctgcaaggtg gtctgcaggt
     5761 agctgcgcag gctgcggtcg aacagccaga ggctgctttg cgccagcagc aggccgataa
     5821 ccagcagtac gctgatcagc cccaggctca aacgccgctg aatcgacctc aaacagcatc
     5881 cccgctaaag cgataaccct gaccgcggcg ggtttcgatg atctcgcgac ccagtttgcg
     5941 ccgcaggtga ttgacgtgga cctcgataac attcgagtcg cgttcgctct cgccgtcata
     6001 caggtgctca gccaggtgac tcttcgataa caactgtccg gcatgcagca tgaaatagcg
     6061 cagcaagcgg aactcggcag ccgtcagctg cacctcttca tcattgcgtt tgactgtttg
     6121 tcgaccttca tccagctgca aacccgcggc ttccagttgt ggctggttgg ccaagccatg
     6181 ggcgcggcgc agcagggact ggatgcgcag ttgcagctct tccgggtgaa agggtttgct
     6241 cagatagtca tccgcgccag ccttgaggcc ttcgatacgt tcggcccagg agccgcgcgc
     6301 agtgaggatc aacaccggtg tggtaaggcc gccagtgcgc cattcctgca acacctcaag
     6361 ccccggtttg ccaggcaagc cgaggtcgag aatgatcaga tcatagggtt ctgtcgcgcc
     6421 ctggtaagcg gcatcacggc cgtcggccaa ccagtccacc gcgtaaccct ggcgggtcaa
     6481 accggccagc agctcatcag ccaggggtac atggtcttca accagcaata ggcgcattca
     6541 gtcttccacc tcatccttga gcacttcgcc ggtgctggca tgcaactcca gctcacgcac
     6601 gatgccttgc tcggtcacca gttcgacttc gtaaaccagt acgccgtcct cttcttccag
     6661 ctcgacttcc agcagaaccg caccgggatg aggcttcaag gccatctgca acaactgctc
     6721 aaggggcatg atcagcccct cgcggcgcag acgtaatgct tcatcctgat ccaggtcacg
     6781 cgcgctgctg ctgaacgtca gcgtcagcag caggcaggcc aggaatgcgc gggcaagcca
     6841 agcagtgctg ttcatcaatc gtctcgttgg ttcttcagaa cttgtgcgtt agtggcatcc
     6901 agttcgacgt cccactgcac accctgggcg tcgcgaactt caacctggta gatatagcgg
     6961 ccgtactctt cttccagctc ggtgtcttcg gtcttgctgc ctgggtgcag cgccagagct
     7021 gcagcgttga gtttttcgaa ggactggatg gtgccggcgt cacgcagttt cagcgcttcg
     7081 tccgggccca ggtcacgggc ttgggccaaa ccggcagtca gggtcagggc agcagcggca
     7141 aatacggcag tcagtgtttt catgttcaag ttccttatgg agtgggcttt cgacggagtc
     7201 caagttactg cacacgactt aactcaagct gaatttgctt aaggcattgc ccaagcactg
     7261 atttatccag acccacgcgt gcatcattca cgcgctgaag tcgcttggcc tggccattca
     7321 gcgctctata atcgctgcct tgcgcgatgg aggccagcat gagtgcgata ttaatcaagt
     7381 cccctgccct gacaattaaa gccggcaaac gcgccctcgc ccgtattcgc gagcacggct
     7441 tgcagcctgc cgacgtcggc attctgccgg gcgcggcagg cggcccgaag gcgctgggaa
     7501 ttcagggact ggatctggcg ctgtttggcg actggctgca acgtgcgacc cgcgagcgct
     7561 cgctgattgg cgcgtccatc ggttcctggc gtttcgccag cgcctgcttg ccagatgcgg
     7621 cagccggact gcgccggctt ggcgagttgt atacctcaca gcgctttgcc aagggcgtga
     7681 gcatggcgca ggtatcgagc agctgcagcc tgatgcttga tcaactgctg ggcaacgacg
     7741 ccgccaacat cctcagtaac ccacactacc gcttgaatat cgtcgtggtg aaaagccatg
     7801 gcctactcca acacgaccat cgcggcgcac tcggcctggg cctgtcttcg gtgattggca
     7861 gcaatctgct gggccgccca cgcctggcca agcacttcga gcgggtgatt ctgcacgatg
     7921 cccgcctggc gccgccgctg ggcgcgctta gcgactttcc gtcgcgctat ctgcaactcg
     7981 atggcggcaa tctgcgccat gcgttgctgg cctcaggctc catcccgatg gtcatgcaag
     8041 gggtgcgcga tattcccggt gcaggccccg gcacctaccg cgatggcggc ctgctcgact
     8101 atcaccttga tctgccatac agcggcgaag atatcgtgct ctacccgcac ttcaccgata
     8161 aggtcatccc cggctggttc gacaagggca tgccctggcg tcgcggcgat gctgggcgtc
     8221 tgcaggatgt agtgctgctg gcgccctcgc gtgactacct ggcgcgcctg ccccatggca
     8281 aactccccga ccgcaaggac ttcagccgtt atctgggcga tgatgccggc cgcgagcgct
     8341 attggcgcac ggccatgagc gagagccagc ggcttggcga cgagtttctc gaactggtcg
     8401 acagcggccg cctcggtgaa cgcctgcaag cactttaata atccgtggcg cgaacatggc
     8461 cgccacgcag acaagaccag ggatgggcag gatgaaactc gcagtcgcca ttatccacgg
     8521 catcggcacg cagcccgacc tgcgtaacga cgaaggtcag cacgacttcg cccagaacct
     8581 tatcgccgga ttacgccagc gcctgggcgt tgaggccaat caagtggcct ttcagaccct
     8641 gcactgggcc agcgtgctcg acaagcggca actggtgttc ctcgatcagc tgcgcgatca
     8701 gccggtacgc tggcgttggt tgcgccgtat cgtcacgatg ttttttggcg atgccagcgg
     8761 ttaccgcaaa gtcagcgagg cctacgacac cacctatgaa gaagtccatc agtgcctacg
     8821 cagcggcctc aacgcattgc gcgccagggt cgatgccgac accccgctgg tggtgctggc
     8881 gcactcactg ggcgggcata tcttctccaa ttttgtctgg gatcagcagc gcatcaacaa
     8941 agccgaaagc tgcccgcttg atccttttct ggctctggaa accctgagtg ggctgatcac
     9001 cttcggctgc aatatcccgc tatttacctt cgcctacgac accgtcgtgc cgatccgctt
     9061 ccccggccac tgcctgaccg aggcggtgac cgaacaggcg cgctggctca acctctatgc
     9121 tcccgccgac ccgctgggtt acccgttgcg gccgctgccc agttacgccg ccgtggtggc
     9181 cgaagatcgc gccatgccgg taggcccctg gtacaagcgg catacgccat tgagccactt
     9241 gggttattgg gacgaccggg gctttcaccg ttatgtcgcc ggttatttgc gccagttact
     9301 ggcggtctgc aaggaccgtg aaagcgtcta aagtccgctt tcctcgcgct gtttctcggc
     9361 ttacgccatt aaaactgtga tttatcggac aatttcttgc atttcttcga caacatgtga
     9421 cttaacagtc agcttcgctc atcaacactt atgactggca agcgtgagtc caccgtttgc
     9481 ccctttgtgt atttgggatc acctcggaga aaccgacccc atgaacgcac cggtgcgttt
     9541 taacgaagct ttgctgattg ccgaccgcgc ctttcaacct ttccaatgcg tcgcctgggc
     9601 cccgcaagat ggcactggta acctaagcct gagtgtgatc gaccgtacca gcactcgcct
     9661 gctcggccgc acccaactgt ccagcaccat ctacagcgac cc
//
LOCUS       DASRUM010000174         3745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_607336, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000174 DASRUM010000000
VERSION     DASRUM010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607336"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..123
                     /locus_tag="VFF22_11150"
                     /pseudo
     CDS             1..123
                     /locus_tag="VFF22_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254186.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
     gene            complement(112..477)
                     /locus_tag="VFF22_11155"
                     /pseudo
     CDS             complement(112..477)
                     /locus_tag="VFF22_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
     gene            544..1029
                     /locus_tag="VFF22_11160"
     CDS             544..1029
                     /locus_tag="VFF22_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZX17409.1"
                     /translation="MTQAPLASRPHDHSHCVSHALAEAESICTRQGLRLTTLRKRVLE
                     LVWASHKPLGAYDILGVLSDEDGRRAAPPTVYRALDFLLENGLVHRIASLNAFVGCSH
                     PEHAHQGQFLICRSCNAAIELEQATISDAIISSAAGVGFAVESQTVEVVGLCAGCQEP
                     T"
     gene            1026..1832
                     /gene="znuC"
                     /locus_tag="VFF22_11165"
     CDS             1026..1832
                     /gene="znuC"
                     /locus_tag="VFF22_11165"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019694476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HZX17410.1"
                     /translation="MSDALIRLTGVGVTFAGQNVLQDVQLSVKPGEIVTLIGPNGAGK
                     TTLVRAVLGLLKPDSGSVWRKPKLRVGYMPQKLHVDATLPLSVLRFLRLVPGVTRADV
                     QAALSEVGAEQVIDSPLQSISGGELQRVLLARALLREPELLVLDEPVQGVDVAGQAEL
                     YRLITQLRDRHGCGVLMVSHDLHLVMSTTDQVVCLNRHVCCSGHPEQVSFDPAFVELF
                     GEDAKSLAVYTHHHDHEHDLHGAVVAETPAGLSIKGPVKPHIHGDGCKHG"
     gene            1825..2613
                     /gene="znuB"
                     /locus_tag="VFF22_11170"
     CDS             1825..2613
                     /gene="znuB"
                     /locus_tag="VFF22_11170"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012020233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HZX17411.1"
                     /translation="MADFLLNALLAGLALALVAGPLGSFVVWRRMAYFGDTLSHAALL
                     GVALGLMLDVSPTLAVTVGCVLLAVLLVTLQSRQPLASDTLLGILAHSTLSLGLVVLS
                     FMQDVRIDLMGYLFGDLLAVGPTDLAWIIGGSALVLLLLALLWRPLLAITVHEELAKV
                     EGLPVAAIRLSLMLLIAVVIAVAMKIVGVLLITSLLIIPAAAAQRHARTPEQMAFGAS
                     LLGIVAVCGGLSLSWFQDTPAGPSIVVSAASLFLLSFALPRRTA"
     gene            2682..3536
                     /locus_tag="VFF22_11175"
     CDS             2682..3536
                     /locus_tag="VFF22_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA5502 family lipoprotein"
                     /protein_id="HZX17412.1"
                     /translation="MKSFVFRGLPLILVLLLAGCQSAPQQPANLPVDDLVSAFRQLDQ
                     SLASGDLSGAESQLGSLQQRAAGDTRLEQYQRQLAEAHLQQGQKALQAGDLDNATKAL
                     SRARSLMPQAPALTTGLDGAIAQAREAELAAAEQQRRAAEQADARAEAASLEQARQLR
                     LAAERQAAAIQATQLPNTPPAEEPAPAKPQARLIDPAAANSVIGMPMLDNQDNERLRS
                     LLDGVAADMVAFNCAVRIEVRQAKDFPWVASLLNARIKRLDPSFSPRMSQVIKPAQVP
                     QLVLSPQR"
BASE COUNT          685 a         1238 c         1160 g          662 t
ORIGIN      
        1 gcagaggcgg agagcatctg cacgcgccag gggctgcgcc tgaccacctt gcgtaaacgc
       61 gtgctggaac tggtctgggc cagccacaaa ccgctgggcg cctacgacat tctcggcgtg
      121 ttgctcagcg ctatcgccga aatggcgcaa ggacatacct ggaacatcct gcacggccac
      181 gcttggctta tcgcggccgg ccaatacgcg cggcaggaag ctttccagat ccgggccgac
      241 ccagtacagc aggtcagcgt cgcgcacgct gcgcacatcg gaggggcgca gcgcgtagtg
      301 gtgcggcgaa gcgcctggcg gcagcagcac atcgggctgg ccgacgccat cctgcacggc
      361 agcggcaatc agctgcagcg gcttgatgct ggtcagcacc cgcacatcgg cctgaaccgc
      421 gaaactcagg ggaaaagtga taaacaacag ggcaaaagcc ggaaaaagac gcaacacggg
      481 gagcactcaa gcgggaagga acgggtaata taataacgtc tctcacctta ttcgtcgttg
      541 cccatgactc aagcgcccct ggcttcacgc ccacacgacc attcccactg cgtcagccat
      601 gccctggccg aggcggaaag catctgcacg cgccagggcc tgcgcctgac caccttgcgc
      661 aaacgcgtgc tggagctggt ctgggccagc cacaagccac tgggcgccta tgacattctc
      721 ggcgtattga gcgatgaaga cggccgccgc gccgcgccgc cgacggtata ccgcgccctg
      781 gatttcctcc tggaaaacgg cctggtgcac cgcatcgcct cgcttaacgc ctttgtcggc
      841 tgcagccacc cggaacacgc tcatcaaggc cagttcctga tttgccgaag ctgcaatgcg
      901 gccatagagc tggaacaggc cacaatcagc gacgccatta tcagcagcgc ggccggagtc
      961 ggctttgccg tggaaagcca gacggttgaa gtggtcggcc tctgcgcagg ctgccaggag
     1021 ccgacatgag cgacgccctg atccgcctca ccggagtggg cgtgaccttc gccgggcaga
     1081 atgtgcttca ggatgtacag ctcagcgtaa agccgggtga aatcgtcacc ctgatcggcc
     1141 ccaacggcgc cggtaaaacc actctggtgc gcgctgtact cggcctgctc aagccggaca
     1201 gcggcagcgt ctggcgcaag cccaagctgc gcgtcggcta tatgccgcag aagctgcatg
     1261 tcgatgccac gttgccgctc tcggtgctgc gttttctgcg cctggtgccg ggcgtaacgc
     1321 gcgcggatgt gcaagcagcg ctgtcggagg taggcgccga gcaggtgatc gacagtccgt
     1381 tgcaaagcat ctccggcggc gaactgcagc gcgtgctgct ggcccgcgcc ttgttgcgcg
     1441 aaccggaact gctggtgctg gatgagccag tgcagggcgt cgatgtcgcc ggccaggccg
     1501 agctgtaccg gctaatcacc cagctgcgtg accgccatgg ctgcggcgtg ctgatggtgt
     1561 cccacgatct gcatctggtg atgagcacca ccgatcaggt ggtctgtctg aaccgccacg
     1621 tctgctgttc cggccaccct gagcaagtca gtttcgatcc ggccttcgtc gaactgttcg
     1681 gcgaggacgc caagagcctg gccgtctaca cccaccacca tgaccatgag cacgacctgc
     1741 acggcgcggt ggtcgctgaa acgcctgccg gcctgagcat caaggggccg gtaaaaccgc
     1801 atattcacgg agatggctgc aagcatggct gattttctgc tcaatgccct gctcgccggc
     1861 ctcgccctgg cgctggtcgc cggcccactg ggctcctttg tggtgtggcg gcgcatggct
     1921 tatttcggcg acaccctgtc gcacgctgca ctgctcggcg tcgccctcgg cctgatgctg
     1981 gatgtcagcc cgaccctggc ggtgaccgtc ggttgcgtgc tgctcgcggt actgctggtg
     2041 accctgcaaa gccgccagcc gctggcctcc gacacattgc tcggcatcct cgcccacagc
     2101 accctgtcgc tgggcctggt ggtgctgagc tttatgcagg atgtgcgcat cgacctgatg
     2161 ggctatctat tcggcgacct gttggccgtc ggcccgaccg acctggcctg gatcatcggc
     2221 ggcagcgccc tggtgctgct gctgctggcc ctgttgtggc ggccactgct ggcgataacc
     2281 gtgcacgaag agctggccaa ggttgaaggc ctgccggtgg ctgcgattcg cctgagcctg
     2341 atgctgctga tcgccgtggt gatcgctgtg gcgatgaaga ttgtcggcgt gctgctgatc
     2401 acctcgctgc tgatcattcc cgccgccgcc gcccaacgcc atgcccgcac cccggagcag
     2461 atggctttcg gcgccagcct gctgggcatc gtcgcggtgt gtggcggact tagcctgtcg
     2521 tggtttcagg acactccggc cggcccatcc atcgtggtca gcgcggcatc actgttttta
     2581 ctgagctttg ccctaccaag acgcaccgcc tgaaaaattg gttgtgtcag gcggcgcagt
     2641 gtagacttgc gcgttttttg cacaattcga gacgcgcagt aatgaaatcg ttcgttttcc
     2701 gtggattgcc cctgattctg gttctgctgc tggccggctg ccaaagcgcg ccacagcagc
     2761 cggccaacct gccggtggat gacctggtca gcgcctttcg ccagctagac cagagcctcg
     2821 ccagcggcga cctgagcggc gccgaaagcc agctcggcag cctgcagcaa cgcgccgccg
     2881 gcgatacccg cctggagcaa tatcagcgcc agctcgccga agcacatctg caacaaggcc
     2941 agaaagccct gcaggccggt gatctggata acgccaccaa agcccttagc cgcgcccgca
     3001 gcctaatgcc ccaggcgccg gcgctgacca caggtctgga tggtgcaatc gcccaggccc
     3061 gtgaagccga actggccgcc gccgaacagc agcgccgtgc tgctgagcag gccgacgccc
     3121 gtgccgaagc cgcaagcctg gagcaggcgc gccagttgcg tttggcagca gaacgccagg
     3181 ccgccgcgat tcaggcgact cagctgccga ataccccgcc agcagaagaa ccagcgccag
     3241 ccaagccgca ggcccggctg atcgacccag ccgccgccaa cagcgtgatc ggcatgccga
     3301 tgctggataa ccaggacaat gagcgcctgc gcagcctgct cgacggcgtc gcggcggata
     3361 tggtcgcgtt caattgcgcg gtgcgtatcg aagtgcgcca ggccaaggat ttcccctggg
     3421 tcgcctcctt gctcaatgcg cggatcaagc gcctcgaccc gagtttcagc ccacgcatga
     3481 gccaggtgat caagcctgct caggtgccgc agctggtact cagcccgcag cgttaaccac
     3541 gttgcgctgc gaccacgacc gggcttgccc ggtcgtttgc attggcgctg gctacactgc
     3601 ccagagcagt cagccatcta atgcaattta gctataacca taggtctaca aagatttttg
     3661 cgacctaaac agcgccgggt agactagccg ccgtttcggc ctacccggca gcccgaaaaa
     3721 cccaaaaggt ttatcccgtg atcaa
//
LOCUS       DASRUM010000175        11238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_923308, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000175 DASRUM010000000
VERSION     DASRUM010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 11238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11238
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923308"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VFF22_11180"
     CDS             complement(<1..735)
                     /locus_tag="VFF22_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020796184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HZX17413.1"
                     /translation="MTTNQPDPIVFGERPLSIEQVVALASRSAPAQLQSDAAYREKIA
                     KGARFLDSLLDKEGVIYGVTTGYGDSCVVAVPLHQVEALPRHLYTFHGCGLGKLLDEA
                     STRAVLAARLQSLCQGVSGVRVELLERLQAFLEFDVLPLIPEEGSVGASGDLTPLSYV
                     AATLSGEREVMFKGERRSAADVHKELGWTPLTLRPKEALALMNGTAVMTALACQAYSR
                     ADYLLKLATRITALNVVALEGNPEHFD"
     gene            complement(716..1654)
                     /locus_tag="VFF22_11185"
     CDS             complement(716..1654)
                     /locus_tag="VFF22_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HZX17414.1"
                     /translation="MSGQQHWAGQRERGSFILMKFTALLARLLGRRLISPLLYLIVLY
                     FYLFGAKARRSIRQYQRNLANWSGRAELQPTRHSVFRHFMSFADTLLDKLDVWSGKLG
                     LEQVTLIDPSNACAQLHQQGRGQMLVAAHLGNLEVCRALAELGEQVTMNVLVHTKHAE
                     QFNRLLGEAGATHLRLIQVSELDAAIMLQLSERLERGEWLAIAGDRVPLKGSRKVSVD
                     FLGKPAAFPQGPWLLAGLLQCPVNLIHCLKIDKRYQVIIEPFAERLHWKRSERDEVIR
                     HWTQRYADQLAQRCLDAPLQWFNFYPFWNEDDDKSA"
     gene            complement(1651..2385)
                     /locus_tag="VFF22_11190"
     CDS             complement(1651..2385)
                     /locus_tag="VFF22_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009046624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZX17415.1"
                     /translation="MHKPCAVIPVYNHEHALPAVVAALHKAGLPCVLVDDASSPACAA
                     VMDQLVTQPDTHLLRLAVNQGKGGAVMAGLREAARLGFSHALQVDADGQHDLSDLPRF
                     IAASQDAPQALICGYPQYDDSVPKGRLYARYLTHVWVWINTLSLSIRDSMCGFRVYPL
                     PATLALIDAVNIGKRMDFDTEILVRLAWRNQPMHWLPTKVHYPLDGLSHFRLWHDNAL
                     ISKMHAKLFFGMLLRAPQILWRRCRA"
     gene            complement(2479..4161)
                     /locus_tag="VFF22_11195"
     CDS             complement(2479..4161)
                     /locus_tag="VFF22_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZX17416.1"
                     /translation="MNWLPLDHLLLEAHPGRAVTADLDHPQLRQHALHLAAELQNRGV
                     KRLALYLDDAAELAIALLAAWRAGIAVLLPADAQPQTRQRMSTQCDLWLDSSGVATFD
                     HLRSVGSVALPPAPLDLDQCQLTLCTSGSSGEAKLIDKSLRQLANEVTALEQLWGAQL
                     GNATILGSVATQHIYGLLFRVLWPLCAGRAFLRRAQPFPEDLQRESLTVTNGFAWVAS
                     PALLKRMGDNLNWPALCAVRQVFSSGGALPAETAAELQSLLGQWPTEIYGSSETGGIA
                     WRQGGELWTPFAGVELGQNDEGALHLTSPYLPTGHREQTADAVELQADSRFALRGRLD
                     RIIKLEEKRISLPMLEQALSSHEWLSDARLGVVQEGRAYLGALVALSPAGLHALRNQG
                     RKTVTETLRRHLAGHCEAIALPRRWRLLAQLPYNNQGKLAQATVEALLAAPRPVQVEP
                     LSAVEQDGEWQLELVVPLDLAHFTGHFPQTPVLPGVVQVDWAQQLARHLIKDLPPRFS
                     GMEVLKFQQLVRPGDQLQLTLRFDAARGKLYFAFRNGEAPCSSGRILLGPAQ"
     gene            complement(4158..4703)
                     /locus_tag="VFF22_11200"
     CDS             complement(4158..4703)
                     /locus_tag="VFF22_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17417.1"
                     /translation="MSRLLGLLLLLAGLLYPFAVYYGMEHLSPRLFAALLGGLWLARA
                     LSQWGKPGNRWMTAVALAFCLLLGLAGEPELLRWYPVLLNGLLLALFGLSLKFGPPLI
                     ERLARLTEPQLPEVAIHYTRLVTAVWAWFFLANGLVVVALNFWAPLSWWTLYTGLIAY
                     GLMGLLFAGEWLVRQRVRRHE"
     gene            complement(4703..4963)
                     /locus_tag="VFF22_11205"
     CDS             complement(4703..4963)
                     /locus_tag="VFF22_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010563238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZX17418.1"
                     /translation="MQTREEIFDTLRDALVELFELEAERVTLNANLYQDLEIDSIDAV
                     DLIDHIKRQTGKKLAAEEFKAVRTVSDVVEAVLQLVNTPAAG"
     gene            complement(4975..5235)
                     /locus_tag="VFF22_11210"
     CDS             complement(4975..5235)
                     /locus_tag="VFF22_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HZX17419.1"
                     /translation="MSDLQLEIKTLIIDALGLEDMAAEDIAADLTLFGEGLGLDSVDA
                     LELGLAIQKRFGIKIDAEAKDTRKHFANVASLAAFVSSQQTA"
     gene            complement(5210..6025)
                     /locus_tag="VFF22_11215"
     CDS             complement(5210..6025)
                     /locus_tag="VFF22_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZX17420.1"
                     /translation="MELVTQQGLSRYSAPYVWRLIATALSFSLFGIGGVLLRVLIFPL
                     LALLPGDALSRRTRARAVVSKTFYLFVRFMYRSGVLTYEVEGIERLGQPGQLVIANHP
                     SLIDVVVLIAFIRDANCVVKQSLWDNPSMRGPLRAAHYISNSGSMDMLDEAAGALQSG
                     QTLVIFPEGTRTTPGHSPEFHRGAAAIALRGARVVTPVVISVSPTTLTKAEPWYSIPS
                     RRFHIRLRIGEDIDPQQFNAMGPPPIASRKLNDFLHQHFMKELAKDERFAAGN"
     gene            complement(5998..6714)
                     /locus_tag="VFF22_11220"
     CDS             complement(5998..6714)
                     /locus_tag="VFF22_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014336218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HZX17421.1"
                     /translation="MTHFRIEQWRAWAPGLDSTADWCAWHQAPARLVDAGQQPDVSAL
                     PAMQRRRLSRLARMAFHVAWPLAEAHPQLSMVFASRHGETPRTLGILTDLAHGEPLSP
                     TQFSLSVHNAIIGLWSIQRGDHSEMTALAGAGDGLENAVLEACGMLHDGAQAVLLVIA
                     EETPPELYAPFIDDVPFPYAVGLLLTLGDDWQLQLSRGEGAGSEWPHALNLVRALCSD
                     QPTLQHHWKSRQWNWSRNKA"
     gene            6942..7715
                     /locus_tag="VFF22_11225"
     CDS             6942..7715
                     /locus_tag="VFF22_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HZX17422.1"
                     /translation="MRRVIFNQKGGVGKSSIACNLAAVSASQGYRTLLIDLDAQANST
                     HYLTGLTGEDIPMGIAEFFKGTLSSAPFGKKGSVDIYETPFDNLHVVTATAELADLQP
                     KLEQKHKINKLRKLLEDLSEDYERIYLDTPPALNFYTVSALIAADRVLIPFDCDSFSR
                     QALYGLLREIEELKEDHNEGLEVEGIVVNQFQPRATLPQQLLDELIAEGLPVLPVNLM
                     SSVKMRESHQACTPLIYLDRNHKLTQQFVELHDLLNSAD"
     gene            complement(7774..8574)
                     /locus_tag="VFF22_11230"
     CDS             complement(7774..8574)
                     /locus_tag="VFF22_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZX17423.1"
                     /translation="MPSHILIAHDLRDTADMALCRATQLAKQFNAKLTILHVLDPSKS
                     SQEHEQSKQALDRSLTQYAPAGSQLLIRTGIKPSEVVLQQLNELGCDLLVLGGHHQRH
                     DFFSGTSLDRIARNCPVPLLLVARNDSRPYERALAAIDFSLCACSALGQAYRLLPEAA
                     ELHALHVFEPDKGTPKQIEAQLTTQKALINQLLEDEAQNLPEKGPTLTHSVEQGGILR
                     CLQARLKSNNTELLVLGSHGSSALSQALLGSLAQHFLHKAPCDIYVVR"
     gene            complement(8663..9106)
                     /locus_tag="VFF22_11235"
     CDS             complement(8663..9106)
                     /locus_tag="VFF22_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HZX17424.1"
                     /translation="MSMPIELVRQLTEEQIAFVQRSGLKAEVLEPGHVRLLMPLAGNQ
                     NHIGSMYAGALFTLAEIPGGALFLTSFDAQRFYPIIKEMNLRFRRPATGDIRVEARLS
                     AEEIQQLQKQATEQGKAEYVLQLQLTDESGEVVVESRGLYQLRAR"
     gene            complement(9213..9647)
                     /gene="trxC"
                     /locus_tag="VFF22_11240"
     CDS             complement(9213..9647)
                     /gene="trxC"
                     /locus_tag="VFF22_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HZX17425.1"
                     /translation="MSNPLLIPCPHCNGLNRTPAERLGDAPRCGRCKAEVLPSTPFEL
                     TQANFNAQNKGGLPLLIDVWASWCGPCRSFAPIYQQAAAQLQGHCRLSKLDSEANPQL
                     SAEMGIRSIPTLILLKGGVEVARQSGALPLPQLLGWLRQQGI"
     gene            complement(9708..10985)
                     /locus_tag="VFF22_11245"
     CDS             complement(9708..10985)
                     /locus_tag="VFF22_11245"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011533350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HZX17426.1"
                     /translation="MKLETLAIHAGYSPDPTTKAVAVPIYQTSSFAFDDTQHGADLFD
                     LKVAGNIYSRIMNPTNAVLEERVAALEGGVGALAVASGMAAITYAIQTVAEAGDNIVS
                     VAKLYGGTYNLLAHTLPRFGIQTRFAAHDDIAALEALIDERTKAVFCESIGNPAGNIV
                     DLAALAAAAHRHGVPLIVDNTVATPILCRPFEHGADIVVHSLTKYIGGHGTSIGGIVV
                     DSGKFPWADNKARFPLLNTPDPSYHGVTYTEAFGPAAFIGRCRVVPLRNTGAALSPFN
                     AFLILQGLETLALRMERHCENALKVAHYLQNHPQVAWVKYAGLPDHPEHALAQRFCGG
                     KPASILSFGIVGGQAAGARFIDALQLVVRLVNIGDAKSLACHPASTTHRQLNDEELER
                     AGVPRDMVRLSIGIEHSDDILADLAQALDAAAG"
BASE COUNT         2044 a         3371 c         3742 g         2081 t
ORIGIN      
        1 gtcgaagtgt tccggattgc cctccagcgc caccacgtta agtgcggtga tacgggtggc
       61 cagcttgagc aggtaatcgg cgcgcgaata ggcctggcag gccagggcgg tcatcactgc
      121 ggtgccgttc atcagcgcca gggcttcttt cgggcgcagg gtcagcggcg tccagccgag
      181 ttccttatgt acgtctgctg cgctgcggcg ctcgcctttg aacatcactt cgcgttcacc
      241 gctgagggtg gcggcgacat aggacagcgg cgtcagatcg ccgctggcgc ccaccgagcc
      301 ttcttccgga atcagcggca gcacatcaaa ttcgagaaac gcctgcaggc gttccagcag
      361 ctccacgcgt acccctgata cgccctgaca cagcgactgc agacgcgcgg cgagcacggc
      421 gcgggtgctg gcttcatcca gcagcttgcc caggccgcag ccgtggaagg tgtacagatg
      481 acgcggcagc gcctcgacct ggtgcagcgg cacggccact acgcaggagt cgccgtagcc
      541 ggtggtgacg ccgtagatca cgccttcctt gtccagcagg ctgtcgagga aacgcgcgcc
      601 cttggcgatt ttttcgcggt aggcggcatc gctctgcagt tgcgccgggg cgctgcggct
      661 ggccagggcg acaacctgct cgatgctcaa ggggcgctcg ccaaacacga tgggatcagg
      721 ctgatttgtc gtcatcttca ttccagaacg ggtagaaatt gaaccattgc aggggcgcat
      781 ccagacaacg ttgcgcgagc tggtcggcgt agcgctgggt ccagtggcgg atcacctcgt
      841 cccgttcgct gcgcttccag tgcaggcgct cggcgaaggg ttcgatgatc acctgatagc
      901 gtttgtcgat tttcaggcag tgaatcaggt tgaccgggca ttgcagcagc ccggccagca
      961 accacgggcc ttgcgggaag gcggcgggct tgccgagaaa gtccacgctg accttgcggc
     1021 tgcccttgag cggcacgcgg tcgccggcaa tcgccagcca ttcgccgcgt tccagacgct
     1081 cggacaattg cagcatgatc gccgcgtcca gttcgctgac ctgaatcagg cgcaggtggg
     1141 tggcaccggc ctcaccgagc aggcggttga actgttcggc gtgtttggtg tgcaccagca
     1201 cgttcatcgt cacctgctcg ccgagttcgg ccagagcacg gcagacttcc aggttgccca
     1261 ggtgtgcggc caccagcatc tgcccgcggc cttgctggtg cagctgcgcg caggcgttgc
     1321 ttgggtcgat cagggtcacc tgttccaggc cgagcttgcc gctccacaca tcgagcttgt
     1381 ccagcagggt gtcggcgaag ctcataaagt ggcggaacac gctatgccgg gttggttgca
     1441 gctcggctcg gccgctccag ttggccaggt tgcgctggta ctggcggatg ctgcgccgcg
     1501 ccttggcgcc gaacaggtag aaatacagca cgatcaggta cagcagcggg ctgatcagcc
     1561 ggcggccgag caggcgtgcc aacagcgcgg taaatttcat caggataaaa ctgccgcgct
     1621 cgcgctgccc ggcccagtgc tgctggccac tcatgcgcga cacctgcgcc agaggatctg
     1681 cggcgcgcgc agcaacatgc cgaagaacag cttggcgtgc atcttcgaaa tcagcgcgtt
     1741 gtcgtgccac aggcgaaagt gtgagaggcc atccagcggg taatgcacct tggtcggcag
     1801 ccagtgcatc ggctgattgc gccaggccag gcgcaccaga atctcggtgt cgaaatccat
     1861 gcgtttgccg atgtttactg cgtcgatcaa cgccagggtg gccggcagcg gatagacgcg
     1921 aaagccgcac atggagtcgc ggatgctcag cgacagggtg ttgatccaca cccacacgtg
     1981 ggtcaggtaa cgcgcgtaga ggcggccttt cggcacgctg tcgtcgtact gcgggtagcc
     2041 gcagatcagt gcctgcggtg cgtcttgcga ggccgcgata aaacgcggca ggtcgctgag
     2101 gtcatgctgg ccatcggcgt ctacctgcag tgcatggcta aagcccagac gcgctgcttc
     2161 acgcagaccg gccatcaccg cgccgccctt gccctgattc accgccaggc gtagcagatg
     2221 ggtatcgggc tgtgtgacca gctgatccat taccgccgcg caggccgggc tggaggcatc
     2281 gtccaccagc acgcagggca ggccagcttt atgcagggct gcaaccacgg cgggcagcgc
     2341 gtgctcgtgg ttatagacag gaataacggc gcagggctta tgcatggagt gacgcaccgt
     2401 aggatgggtg gagcagcgcg atacccatcg ccgacctgtt gggtatcgct gcgctcaacg
     2461 ccaacctaca aaagccattc attgcgctgg ccccagcaaa atccgtccgg acgagcaggg
     2521 tgcttcgccg ttgcggaagg cgaagtacag cttgccgcgc gccgcgtcga aacgcagggt
     2581 cagttgcagc tgatcgccag ggcgcaccag ttgctggaat ttcagcactt ccatgccgct
     2641 gaaccgtggt ggcaggtcct tgatcaggtg gcgcgccagt tgctgcgccc agtcgacctg
     2701 caccacgccc ggcagcaccg gggtctgcgg aaagtggccg gtgaagtgtg ccaggtccag
     2761 cggtaccacc agctccagct gccattcgcc gtcctgttcg acagccgaca gtggctccac
     2821 ctggaccggg cgtggcgcgg ccagcagcgc ttccaccgtg gcttgcgcca gcttgccctg
     2881 gttgttgtag ggcagttgcg caagcaggcg ccagcgccgg ggcagggcga tggcttcgca
     2941 atggccggcc agatgccgac gcagggtttc ggtgacggtc ttgcgcccct ggttgcgcag
     3001 cgcatgcagg ccggccgggc tcaaggccac cagcgcgccg aggtaggcgc ggccttcctg
     3061 caccacaccc aggcgcgcgt cgctgagcca ttcatggctg cttagcgcct gttcgagcat
     3121 gggcagggaa atgcgttttt cttccagctt gataatccgg tccaggcgac cgcgcagggc
     3181 gaagcggcta tcggcctgca gttccaccgc atcggcggtc tgctcgcggt ggccggtcgg
     3241 cagatagggc gatgtcaggt gcagcgcacc ttcgtcgttc tgccccagct cgaccccggc
     3301 gaagggcgtc cacagctcgc cgccctgacg ccaggcgata ccgccggttt ccgagctgcc
     3361 gtagatttcc gtgggccatt ggccaagcag actctgcagc tcggcggcgg tttctgctgg
     3421 cagtgcgccg ccggaggaga acacctgacg caccgcacac agcgccggcc agttaaggtt
     3481 gtcgcccatg cgtttcaata gtgcagggct agcgacccag gcaaagccat tggtaacggt
     3541 caggctttca cgctgcaggt cttcggggaa cggctgggca cgacgcagaa aggcgcggcc
     3601 ggcgcacagc ggccagagca cgcgaaacag caggccgtag atatgctggg tggcgacgct
     3661 gccaaggatg gtcgcgttgc ccagttgcgc gccccatagc tgttccaggg cggtgacttc
     3721 attggctagc tggcgcaggg atttgtcgat cagtttggct tcgccgctgg aaccggaggt
     3781 gcacagggtt agctggcatt ggtcaagatc gagcggtgct ggcggcaatg caacgctgcc
     3841 gacgctacgc aggtgatcaa acgtagcgac gcccgagcta tctagccaca ggtcgcactg
     3901 cgtactcata cgttggcggg tctgcggctg cgcatcggcc ggcagcagta cggcaatccc
     3961 ggcgcgccag gcggccagca gggcaatggc cagctcagcg gcatcgtcga ggtacagcgc
     4021 caggcgtttt acgccacggt tttgcagctc agccgccaag tgcagggcgt gctggcgcag
     4081 ctgggggtga tccaggtctg cggttaccgc gcgaccgggg tgggcttcga gcagcagatg
     4141 atcgaggggc agccagttca ttcatgtctc cttacacgct ggcgtaccag ccattcaccg
     4201 gcaaacaaca ggcccatcag gccgtaggca atcagcccgg tgtacagggt ccaccagctc
     4261 agtggggccc agaagttcag cgccaccacc accaggccat tggccaggaa gaaccaggcc
     4321 cagaccgcgg tgaccaggcg tgtgtagtgg atcgccacct ccggcagttg cggctcggtc
     4381 aggcgcgcca ggcgttcgat cagcggcggg ccgaacttca ggctcaggcc gaacagcgcc
     4441 agcagcaggc cgttgagcag caccggatac cagcgcagca actcaggctc cccggcgagg
     4501 cccagcagca ggcagaacgc cagggccacc gcggtcatcc agcggttgcc gggcttgccc
     4561 cattggctga gcgcgcgggc cagccacaga ccgccgagca acgcggcgaa caggcgtggc
     4621 gacaggtgct ccatgccgta atacacggca aaggggtaga gcagccccgc cagcagcagg
     4681 agcaggccca gtaggcgact cattatccgg cagcgggcgt attgaccagc tgcagcaccg
     4741 cctcgaccac gtcgctgacg gtgcgtaccg ccttgaactc ctcggccgcc agcttcttgc
     4801 cggtctggcg cttgatatgg tcgatcaggt cgaccgcatc gatgctgtct atctccaggt
     4861 cctgatagag gttggcgttc agggtgacgc gttcggcttc gagttcgaac agttcgacca
     4921 gggcgtcgcg cagggtgtcg aaaatctctt cacgggtttg catcggtggc ttcctcaggc
     4981 ggtctgttgc gatgacacga atgccgccag gctggctacg ttggcaaagt gcttgcgggt
     5041 gtctttggct tcggcgtcga tcttgatgcc gaaacgtttc tggatggcca ggccgagttc
     5101 cagggcgtct accgaatcca gccccaggcc ttcaccgaac agggtcaggt cggcggcgat
     5161 gtcttcggcg gccatatcct caaggcccag agcgtcgata atcagggttt taatttccag
     5221 ctgcaaatcg ctcatctttg gcgagctcct tcataaagtg ttggtgcaga aaatcgttga
     5281 gtttgcgtga ggctatcggc ggcggcccca tggcattgaa ttgttgtggg tcgatatcct
     5341 cgccgatgcg caggcgaata tggaaacggc gcgatggaat gctgtaccag ggctcggctt
     5401 tggtcagggt ggtggggctg acgctgatca ccaccggggt gaccacgcgc gcgccacgca
     5461 gggcgatggc cgctgcgccg cggtgaaact ccgggctgtg tcccggtgtg gtgcgcgtgc
     5521 cttcggggaa gatcaccagg gtctggccgc tttgcagggc gccggccgct tcatcgagca
     5581 tgtccatgct gccgctgttg ctgatgtagt gggccgcgcg cagtggcccg cgcatgcttg
     5641 ggttatccca gaggctctgt ttgaccacgc agttggcgtc gcgaataaag gcgatcagca
     5701 ccaccacgtc gatcaacgac gggtgattgg caatcaccag ttgccctggc tggccgaggc
     5761 gttcgatacc ttcaacttcg taagtcagca cgccgctgcg gtacataaag cgcacgaaca
     5821 ggtagaaggt cttgctcact actgcgcggg cgcgggtgcg ccggcttagg gcatcacccg
     5881 gtagcagggc gagcagcggg aagatcagca cgcgcagcag tacgccgccg atgccgaaca
     5941 ggctgaaact cagggccgtg gcaatcagcc gccagacata gggcgcgctg taacggctca
     6001 ggccttgttg cgtgaccagt tccattgtcg gctcttccag tggtgctgca gggtcggctg
     6061 gtcgctgcac agggcgcgga ccaggttgag agcgtggggc cattcgctgc cggcgccttc
     6121 gccccggctt agttgcagct gccagtcatc acccagcgtc agcagcaggc cgacggcata
     6181 gggaaacggc acgtcatcga taaaaggcgc gtaaagctcg ggaggggttt cctcggcaat
     6241 caccagcagc accgcctgcg cgccgtcgtg gagcatgccg caggcttcca atacggcgtt
     6301 ttccaggcca tcaccggcgc cggccagagc ggtcatttcg ctgtgatcgc cgcgctggat
     6361 cgaccacagg ccgatgatcg cgttatgcac cgagaggctg aactgggtcg gcgacagcgg
     6421 ctcgccatgc gccagatcag tgagaatgcc caaggttcga ggggtttcgc cgtgccggct
     6481 ggcgaacacc atggacaatt gcgggtgcgc ttcggccagc ggccaggcga cgtggaaagc
     6541 catccgtgcc agtcggctca agcgccggcg ctgcatggcc ggcagtgcgc tgacatccgg
     6601 ttgctgtcca gcatcgacca ggcgtgcggg tgcctgatgc caggcgcacc agtcagccgt
     6661 gctgtccagg ccaggagccc aggcacgcca ttgttcgatc ctgaaatgtg tcacgcggtg
     6721 cgcgtccgtg tcggctatag ccagtcgcag ccagcgaagg gcttggcagc aggtgtggcg
     6781 aagtgctggc attatccaga ccgcgcgcgc cgtgcgcaaa tccgtggcat accgttctga
     6841 ccggaggctg cgcggttttg ccgaaaatct cgcccttgtc acgtactttg catagaatcc
     6901 gggaccattt cggggtttat gcgggattca gggggtgttt gatgcggcgc gtgatcttca
     6961 atcagaaggg cggtgtgggt aagtccagca ttgcctgcaa cctggccgcg gtcagcgctt
     7021 cccagggcta tcgcacgctg ctgatcgacc tggacgccca ggccaattcg acccattacc
     7081 tcaccggttt gaccggcgag gacattccca tgggcattgc cgagttcttc aagggcacgc
     7141 tgtcatcggc gccgttcggc aagaaaggca gcgtggatat ctacgaaacg ccgttcgata
     7201 acctgcatgt ggtcaccgcc accgccgagt tggccgacct gcagcccaag ctggagcaga
     7261 aacacaagat caacaaactg cgcaaactgc tcgaagacct cagcgaggat tacgagcgga
     7321 tttacctgga tactccgccg gcgctgaatt tctacaccgt ctccgccctg atcgccgctg
     7381 accgtgtgct gattccgttt gactgcgaca gcttctcgcg ccaagcgctt tacggcctgc
     7441 tgcgcgaaat agaagaactc aaggaagacc acaacgaagg cctggaagtc gaaggcatcg
     7501 tggtcaacca gttccagccg cgcgccacgc taccgcagca gctgctcgat gaactgatcg
     7561 ccgagggcct gccggtgctg ccggtcaatc tgatgagttc ggtgaagatg cgcgaatcgc
     7621 accaggcctg cacgccgctg atctacctgg accgcaacca caagctgacc cagcagtttg
     7681 tcgagttgca tgacttgctt aacagcgctg actgagccga gctcgaaaaa caaaaaagct
     7741 gcctaggcag ctttttttgt tgggcgtgga aacctagcgg acgacgtaga tatcgcaagg
     7801 cgccttgtgc aggaagtgct gtgccaggct gccgagcagg gcctgcgaca gtgcgctgct
     7861 gccgtggctg ccgagtacca gcaattctgt gttgttgctt ttcaggcgcg cctgcaggca
     7921 acgcaggatg ccgccttgct ccaccgagtg ggtcagggtt gggccttttt ccggtaggtt
     7981 ctgcgcttca tcttccagca gctggttgat cagggctttc tgcgtggtca gttgcgcttc
     8041 gatctgcttg ggcgtgccct tgtccggctc gaagacgtgc aacgcatgca gctcggcagc
     8101 ttctggcagc aggcggtagg cctgaccgag ggcgctgcag gcgcagagcg agaaatcgat
     8161 agccgccagg gcgcgttcat acgggcgtga atcgttacgc gcgaccagca acaacggcac
     8221 cgggcagtta cgagcaatgc gatcgaggct ggtgccggaa aagaagtcgt ggcgctggtg
     8281 atggccgccg agtaccagca ggtcgcaacc cagctcatta agctgctgca gcaccacttc
     8341 cgagggcttg atgccggtgc ggattagcag ctggctgccg gctggggcgt actgggtcag
     8401 gctgcggtcc agcgcctgtt tggactgctc atgttcctgg ctacttttac tcgggtcgag
     8461 cacatgcaga atggtcagct tggcgttgaa ctgcttggcc agctgggtgg cgcgacacag
     8521 cgccatatcc gcagtgtcgc gcagatcgtg ggcaataaga atgtggctgg gcatggtcgg
     8581 cgttctcctg ccgatgcatg tcggcacatt tattgtgagc tcgagtcata gctggagttt
     8641 tgacctaagg caaggtcagg cgtcagcgtg cgcgtaattg atacagaccg cgactctcga
     8701 ccactacttc accactctca tcggtcagct gcaactgtaa aacatactcg gctttgccct
     8761 gttctgttgc ctgcttttgc agctgctgga tttcttccgc gctaaggcgt gcctcgaccc
     8821 gaatatcgcc ggttgctggg cggcgaaagc gcaggttcat ttccttgatg atggggtaga
     8881 aacgctgggc atcgaagctg gtgagaaaca gggcaccgcc gggtatttcc gccagggtga
     8941 acagcgcacc ggcgtacatg ctgccgatat ggttctggtt gcccgccagc ggcatgagca
     9001 ggcgcacatg gccgggctcc agcacttcgg ccttgagccc gctgcgttgg acaaaggcga
     9061 tttgttcttc ggtaagttgg cgtaccagtt cgataggcat cgacattgtt cggtctcctg
     9121 ggaaagtgtc ggatcagcct aaaggttgct cgagggtcgg gtaatgaccg cggtgtccgc
     9181 gtgaggttga ccgattcgct cagccttgcc cgtcagatac cttgctggcg caaccagcca
     9241 aggagttgcg gcaagggcag ggcgccgctc tgccgggcaa cctctacgcc gcccttgagc
     9301 aggatcagcg tgggaatgga acggataccc atttccgccg acagctgcgg gttggcttcg
     9361 ctgtccagtt tgctcaggcg gcagtggccc tgcagctggg cagcggcctg ttgatagatg
     9421 ggcgcaaagc tgcggcacgg cccgcaccaa ctggcccaca catcgatcaa cagcggcagg
     9481 ccgcccttgt tctgcgcgtt gaagttggcc tgggtcagct cgaaaggtgt gctcggcagc
     9541 acctcggcct tgcagcggcc acagcgcggc gcatcgccca ggcgctcggc gggagtgcga
     9601 ttgaggccat tgcagtgcgg gcaggggatc agtagagggt tggacatggg cgactccaga
     9661 ggcggttatc ccttatctgg agtcgcttgc gcggatatca agggcgatca gcccgccgca
     9721 gcgtccaggg cctgggccag gtcggcgagg atgtcgtcgc tgtgctcgat accgatcgac
     9781 aggcgcacca tgtcgcgtgg cacgccggca cgctccagct cttcgtcatt cagctggcga
     9841 tgggtggtcg aggccgggtg gcaggccagc gatttggcat cgccgatatt caccaaacga
     9901 accactaact gcagcgcatc gataaagcgc gcgccggcag cctggccgcc gacgatgcca
     9961 aaggacagga tcgaggccgg tttgccgccg cagaaccgtt gcgccagggc gtgctcgggg
    10021 tgatcgggca ggccagcgta ttttacccag gccacctgcg gatgattttg caggtaatga
    10081 gcgaccttca gcgcgttctc gcagtggcgc tccatgcgca aggccagggt ttccaggcct
    10141 tgcagaatca ggaaggcatt gaacggcgac agcgccgcac cggtgttgcg cagcggcacc
    10201 acacggcagc ggccgataaa ggccgcaggg ccgaaggctt cggtgtaggt cacgccgtgg
    10261 taggacgggt ctggggtgtt gagcagcggg aagcgcgcct tgttatccgc ccaggggaac
    10321 ttgccggagt cgacgacgat gccgccgatg ctggtgccgt ggccgccgat gtatttggtc
    10381 agcgagtgca cgacgatgtc ggcgccatgc tcgaacgggc ggcagaggat cggcgtggcc
    10441 acggtgttgt cgacgatcag cggcacgcca tggcggtgcg cggcagcggc cagggcggcg
    10501 aggtcgacga tattgccagc ggggttgccg atggactcgc agaacactgc cttggtgcgc
    10561 tcgtcgatca gcgcttcgag ggcggcgatg tcgtcatggg cggcgaagcg ggtctggatg
    10621 ccgaagcgcg gcagggtgtg ggccagcagg ttgtaggtac cgccgtagag cttggccacc
    10681 gagacgatgt tgtcaccggc ttcggcgacg gtctgaatcg cgtaggtgat cgcggccatg
    10741 cccgaagcca cggccagcgc gccgacgccg ccttccagcg cagccacgcg ctcttcgagc
    10801 accgcgttgg tcgggttcat gatccgcgag tagatgttgc cggcgacctt caggtcgaac
    10861 agatcggcac cgtgctgggt gtcgtcgaag gcaaaggagc tggtctggta gatcggcacc
    10921 gccacggcct tggtggtcgg atcagggctg taaccggcgt ggatggccag ggtttccaat
    10981 ttcatgcgcg cgttccttca gggcagattg aaagatacgc agcatggaga aagcagcaag
    11041 gcccggtcaa tgcgctggcg caatgaaacg ggccttgggt tagatcgata tattcgccgc
    11101 ttatgccgtc aaaccggcgt cagcgggaag aaccagggaa tgacaaagct ggccaccacc
    11161 cacagcagca ggttgagcgg aatgccgacc ttcataaagt cggtaaagcg ataaccgccg
    11221 gcgttgtaga cgaaggtg
//
LOCUS       DASRUM010000176        59021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_752352, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000176 DASRUM010000000
VERSION     DASRUM010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 59021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59021
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752352"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFF22_11250"
     CDS             <1..385
                     /locus_tag="VFF22_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003303317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HZX17427.1"
                     /translation="ENQRYDVIVVNYANGDMVGHTGVFSAAVKAVECLDSCVGRIVEA
                     LDKVGGEALITADHGNVEQMEDDSTGQAHTAHTCEPVPFIYVGKRPAHIREGGVLADV
                     APTLLTLMGLPIPPQMTGTSIIELG"
     gene            720..1961
                     /locus_tag="VFF22_11255"
     CDS             720..1961
                     /locus_tag="VFF22_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HZX17428.1"
                     /translation="MFRALAVIFLTVLLTPAIADEKSDAQKQLDAARADVSELKQLLE
                     KLQQEKSGVQKDLKKTESEMGQLEKQVKGLQQDLKKSEEEIRRLDSEKKKLEGARLEQ
                     QRLIGIQARAAYQSGRQEYVKLLLNQQNPEKFSRTLTYYDYLSEARMEQLAAFNETLR
                     QLANVELDIANQQRQLLEQKSTLDSRREQLASVRKERQLALAKLNKEFSARDQKLKAR
                     QQEQANLARVLKTIEETLARQAREAEAARQQALLAEREQPRSSTNPAPTGPLVSSGAS
                     FGGPFAKARGKLPWPVDGRLVARYGTPRGGDARTKWDGVLIGASAGSQVHAVHGGRVV
                     FADWLRGAGLLVILDHGNGYLSLYGHNQSLLKDAGDVVKAGEAIATVGNSGGQDTPAL
                     YFAIRQQGRPIDPAQWCRAQG"
     gene            2002..3303
                     /locus_tag="VFF22_11260"
     CDS             2002..3303
                     /locus_tag="VFF22_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZX17429.1"
                     /translation="MPHLFRLTTLALAITLLGSTAVFAAPAEAPATVNDSAPLPLDDL
                     RTFAEVMDRIKSAYVEPVSDKTLLENAIKGMLSNLDPHSAYLEPEAFRDLQESTSGEF
                     GGLGIELGSEDGFLKVISPIDDTPASAAGIQPGDLIVKIDGQPTKGLSLMEAVEKMRG
                     KAGSDIELTLVREGGKPFDVELTRAVIKVKSVKSQLLDDGYGYVRITQFQVNSGEEVG
                     KALAKLRKENGKKLRGLVLDLRNNPGGVLQAAVEVTDHFLKKGLIVYTEGRIANSELR
                     FNADPADASEGVPLVVLINGGSASASEIVAGALQDHKRGVLMGTDSFGKGSVQTVLPL
                     NNDRALKLTTALYFTPNGRSIQAQGIVPDIEVARAKLTREQGDETIKEADLQGHLGNG
                     NGGADKPSKSKTGTPARPQDDDYQLSQALNLLKGLSVTRGD"
     gene            complement(3416..4171)
                     /locus_tag="VFF22_11265"
     CDS             complement(3416..4171)
                     /locus_tag="VFF22_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX17430.1"
                     /translation="MLKTLKASLLFGLVLSACAARAELPADYKVVLLTENFPPFNMAV
                     DDKNYARDDGIDGISAEIVREMFKRAGINYSLTLRFPWDRLYRLTLDKPNYGLFSTTF
                     TPERQPLFKWVGPLAKTGWVLLAAPGNNLSVASLKDAAQYQIGAYKNDAVSQHLESQG
                     LSPINALRDQENIKKLLSGKIDLWATTDPVGRYLAKQEGVSGLNTALRFNEAELYLAL
                     NKDTPDEVVERLQKALDELRAEGFVDEMTNNYL"
     gene            complement(4309..5064)
                     /locus_tag="VFF22_11270"
     CDS             complement(4309..5064)
                     /locus_tag="VFF22_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX17431.1"
                     /translation="MFKQLLIALTGTLMVLSGSARAEVDPNYSLVLLTENFPPYNMAI
                     NGKNFAHEDNIDGIAVDIIREMFKRAGIQYSMTLRFPWDRIYKLALEKPGYGVFVAAR
                     LPEREALFKWVGPIGPDDWVLLGKADSSITLNSLEDAKQYKVGAYKGDAIAEHLVEKG
                     LEPVTALRDTENAKKLMAGQIDLWATGDPAGRYLAKQEGVSGLKTILRFDSAELYLAL
                     NKEVPDEVVQKLQAELDKMRSEGFVDDTLNNYL"
     gene            complement(5347..6117)
                     /gene="hisF"
                     /locus_tag="VFF22_11275"
     CDS             complement(5347..6117)
                     /gene="hisF"
                     /locus_tag="VFF22_11275"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HZX17432.1"
                     /translation="MALAKRIIPCLDVDNGRVVKGVQFENIRDAGDPVEIARRYDEQG
                     ADEITFLDITASVDGRDTTLHTVERMASQVFIPLTVGGGVRAVQDIRNLLNAGADKVS
                     INTAAVFTPEFVGEAASRFGSQCIVVAIDAKKVSKPGETPRWEIFTHGGRKPTGLDAV
                     LWAKKMEDLGAGEILLTSMDQDGVKSGYDLGVTRAISEMVGIPVIASGGVGNLQHLAD
                     GIIEGKAAAVLAASIFHFGEYTVPEAKAYMAARGIVVR"
     gene            complement(6206..6949)
                     /gene="hisA"
                     /locus_tag="VFF22_11280"
     CDS             complement(6206..6949)
                     /gene="hisA"
                     /locus_tag="VFF22_11280"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HZX17433.1"
                     /translation="MLIIPAIDLKDGACVRLRQGRMEDSTVFSDDPVSMAAKWVEGGC
                     RRLHLVDLNGAFEGQPVNGEVVTAIAKRYPHLPIQIGGGIRSLETIEHYVKAGVSYVI
                     IGTKAVKDPAFVAEACKAFPGKIIVGLDAKDGFVATDGWAEISTVQVIDLAKQFEADG
                     VSAIVYTDIAKDGMMQGCNIPYTVALANATRIPVIASGGIHNIGDIQALLDTNTPGII
                     GAITGRAIYEGTLDVAEAQALCDLKYKDE"
     gene            complement(6986..7246)
                     /locus_tag="VFF22_11285"
     CDS             complement(6986..7246)
                     /locus_tag="VFF22_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008080554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 domain-containing protein"
                     /protein_id="HZX17434.1"
                     /translation="MSRAKNKPPLMTLNAEQEREACLVLKRFMAERFELELGSFEAAE
                     VLELFAREIAPHYYNRAIFDVQTHLKERFESIESDLWALEKS"
     gene            complement(7247..7885)
                     /gene="hisH"
                     /locus_tag="VFF22_11290"
     CDS             complement(7247..7885)
                     /gene="hisH"
                     /locus_tag="VFF22_11290"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HZX17435.1"
                     /translation="MQTVAVIDYGMGNLHSVAKALEHVGAGKVLVTSDAQVIREADRV
                     VFPGVGAIRDCMAEIKRLGFDSLVREVSADRPFLGICVGMQALLEHSEENDGVDCIGL
                     FPGKVRFFGKGMVEDGEPLKVPHMGWNEVAQSVKHPLWHNIPDLARFYFVHSYYIEAG
                     NSKQVVGRGHYGKDFAAALADGSRFAVQFHPEKSHTHGLQLLQNFAAWDGRW"
     gene            complement(7886..8479)
                     /gene="hisB"
                     /locus_tag="VFF22_11295"
     CDS             complement(7886..8479)
                     /gene="hisB"
                     /locus_tag="VFF22_11295"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HZX17436.1"
                     /translation="MAERTASVERNTLETQIKVSINLDGTGKAKFDIGVPFLEHMLDQ
                     IARHGLIDLDIECKGDLHIDDHHTVEDVGITLGQAFTKAIGDKKGMTRYGHSYVPLDE
                     ALSRVVIDFSGRPGLQMHVPFTRAVVGGFDVDLFQEFFQGFVNHALVSLHIDNLRGTN
                     THHQIETVFKAFGRALRMAVELDPRMAGQMPSTKGCL"
     gene            8611..9906
                     /locus_tag="VFF22_11300"
     CDS             8611..9906
                     /locus_tag="VFF22_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003296259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HZX17437.1"
                     /translation="MHERKMPATLRFMLAARRSELLGLEDLSQTCELVTRISQLVHAL
                     QKERGYSNLYLSGNAPHQRLQLDGLSQAADSLERQARDDLERIDQNGLGSADKTRLFT
                     RIAYALHSLDELPALRRKIREHRISMQDATAALVRLIGGLLAVVFEAADTAIDPAITR
                     SLVALFNFMQGKELAGQERALGVSGFASGYFTAEMLERLEHLLECQQRSFATFSRFAS
                     PAALELWQSLCASPSNAQACRLREIARHTHADARVDCQLGELWFELHTARIDAMKSVE
                     TCLEQDLLQRCQQSMQRVRRDLHSHRRLLDGLSDMHAAAEQARLFSVHNSDCDSLTEE
                     SPSANLARSTLDLLQSQTQRLQGLHDELREARQALDERKLVERAKQLLIKQHGCSESE
                     AYAQLRQAAMNQSLRLDEVAQRLLARSIEAPMTSPRQKH"
     gene            10220..11551
                     /locus_tag="VFF22_11305"
     CDS             10220..11551
                     /locus_tag="VFF22_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HZX17438.1"
                     /translation="MTDKPSNDDSNSVLMASRRRFLKQSIVAASGIALFGGMGISSQS
                     SAWAAGGEVETTKAKLGFIALTDAAPLFVAAEKGFFAKYGMTEVEVLKQSSWGTTRDN
                     LVLGSAKNGIDGAHILTPMPYLISAGIVTPNNQPVPISILARLNLAGQCISVGEEYRD
                     LKIGVDSTPFKAALEAKKASGKKVSAAMTFPGGTHDLWMRYWLAAGGIDPNNDISTIT
                     VPPAQMVANMKVGSMDTFCVCEPWNAQLINQKIGYTANTTGELWHNHPEKSFALRSDY
                     VAANPKAAQALTMAIMEAQMFCELPENKEEVAKICSQRRWIGAPYADIVDRMKGNFAY
                     GTGKVVENHPEQMRYWDDFASYPFQSHDLWFLTENQRWGYLPRDFDSQALIAQVNRED
                     IWRAAAAALSLPAEQIPTSTSRGIEKFFDGKVFDPENPQAYLDSLTIKAMA"
     gene            11583..12455
                     /gene="ntrB"
                     /locus_tag="VFF22_11310"
     CDS             11583..12455
                     /gene="ntrB"
                     /locus_tag="VFF22_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HZX17439.1"
                     /translation="MNANVKIEPAAVASPATPATPSRLSRIGTSLQQTLLQDLLPPLV
                     ILLILGLVWEMLCSGAGAALPPPSQVISETWELIIDPFYDNGGNDVGLAWQLLASLQR
                     VAVGYLLAVAAGVALGVLIGQSDWAMRGLDPLFQVLRTVPPLAWLPLSLAGFQDSHPS
                     AIFVIFITAIWPIIINTSVGIRNIPQDYRNVAQVIQLNAWEYFHLIMLPAAAPYIFTG
                     LRIGVGLSWLAIIAAEMLIGGVGIGFFIWDAWNASRISDIILALVYIGIVGFCLDRLV
                     AFVGQRITRGIPAA"
     gene            12466..13248
                     /locus_tag="VFF22_11315"
     CDS             12466..13248
                     /locus_tag="VFF22_11315"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX17440.1"
                     /translation="MSYLSVEQLDMSFNRGKVASQVLKGINLRIDQGEFISIIGHSGC
                     GKSTVLNIIAGLLEPSLGVVILDGKEVNGPGPDRGMVFQNHSLLPWLTVFENVAVGVN
                     KVFKRSMSKAERREWVEHNLKLVNMGHALHKYPQEISGGMKQRVGIARALAMKPKVLL
                     LDEPFGALDALTRAHLQDEVMRIQADLGNTMMMITHDVDEAVLLSDRIVMMTNGPSAT
                     IGEILSVDLPRPRDRIALADDHEYNRCRRAVLSFLHDRHRLQ"
     gene            13601..14701
                     /locus_tag="VFF22_11320"
     CDS             13601..14701
                     /locus_tag="VFF22_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZX17441.1"
                     /translation="MDSIDTLIIGAGAIGLACAARLARPSQSTLIAEAEGLIGSHTSS
                     RNSEVIHAGIYYAPGSLKAEVCLEGRERLYAWCHAHQVAHCQLGKLLVAVKSAEIAKL
                     EQLQSNAQACGVHDLQPIEQKRLHALEPAVRGVAALFSPSTGIIDSHAYLQSLLAAAE
                     NRGAQLVLHTRVEQLEASSDGWIASGHSSGEPFQLKAQQVVNAGGLFAQQLANQTQGL
                     QNVPPLHLCQGRYFSYSGRSPFQHLIYPMPEANTTGLGIHATLDLGGQLRFGPDTRYL
                     TQIDYQVDETLREPFAEAIRRYFPTIDSARLVPGYSGIRPKLAGPGEPAADFIIQTPN
                     EHGLPGLVNLLGIESPGLTASLAIAERVAQSL"
     gene            14825..17056
                     /locus_tag="VFF22_11325"
     CDS             14825..17056
                     /locus_tag="VFF22_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HZX17442.1"
                     /translation="MKALGKILGLFFLGLLLIIVALGFALTHLFDPNDYKDEIRQLAR
                     DKANLELTLKGDIGWSLFPWLGLELTDATLASLNTPDKPFADLRLLGLSVRVLPLLRK
                     EVQMSDIRIDGLNLNLQLDEKGRSNWEEVGKPAKVATTATDASVASSDSTPANSTAAE
                     PESSQPLKLDIDSLIVNSARIDYHDAQKGQQFSAESIQLTTGAIREGAGIPIKLSAFF
                     GLNKPVLRARSELQGELRFDRALKRYQLEDAKLSGEVSGEPFDGQTLTYSAQGQLLVD
                     LAAQVAEWNGLKFSGNQLRALGELKVRDLDKQPKLSGGLSIAPLNLREFLATIGQKLP
                     PMTDDTTLSQFELVTRLSGTDNSLTLEELKLTLDDSHFSGQLAIADFAKQTLRVQLKG
                     DQLNLDRYLPAPSKDAKDAGAARKAEVKASVASAGQSGSTPLPDAPTQHAWSSSEVLP
                     IETLRKLDMQLDLGLDQLTAQQQVISNFSAKAQSKGGLLTLQDVRGNIGNGSFDAKAS
                     IDVRPAIPLLSVQKNLSNIPVEPFLKKPDQPSPVKGLLDFKANLTTSGNSQRAWVDGL
                     TGDASFLLNDGVLVDANLEQQLCRGIATLNRKALSSDPRGKDTPFETLQGSLILRNGV
                     AHNPDLKAVIPGLAVSGNGDLDLRILGLDYRVGITITGDQREMPDPACQVNERYVGIE
                     WPVRCRGPLEMGGKACRLDQDGLGKVAAKLAGNKLNEKIEEKLGDKVSPELKDALKGL
                     FNR"
     gene            17053..18126
                     /gene="mutY"
                     /locus_tag="VFF22_11330"
     CDS             17053..18126
                     /gene="mutY"
                     /locus_tag="VFF22_11330"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HZX17443.1"
                     /translation="MSPEQFSDAVLNWYDSHGRKDLPWQQGITPYRVWVSEIMLQQTQ
                     VSTVLGYFDRFMAALPTVNDLAAAPEDEVLHLWTGLGYYTRARNLQKTAQIVMREYAG
                     EFPRDVDQLTELPGIGRSTAGAIASLSMGLRAPILDGNVKRVLARYVAQEGYPGEPKV
                     AKQLWGVAERFTPHARVNHYTQAMMDLGATLCTRSKPSCLLCPLKSGCQAHLLGLEIR
                     YPIPKPRKALPQKRTLMPIFANRHGEVLLYRRPSSGLWGGLWSLPELDEATAIASLAS
                     RHTLQLGAQRELSGLTHTFSHFQLAIEPWLIQVDAAPAAVAEADWLWYNLATPPRLGL
                     AAPVKKLLKRAADALAANTGETP"
     gene            18123..18395
                     /locus_tag="VFF22_11335"
     CDS             18123..18395
                     /locus_tag="VFF22_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HZX17444.1"
                     /translation="MTRTVQCRKHKQELPGLERPPYPGAKGEDIFNNVSKQAWDEWQK
                     HQTLLINERRLNMMNAEDRKFLQVEMDKFLSGEEYAQADGYVPPSE"
     gene            18616..18691
                     /locus_tag="VFF22_11340"
     tRNA            18616..18691
                     /locus_tag="VFF22_11340"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18649..18651,aa:Phe,seq:gaa)
     gene            18823..19068
                     /locus_tag="VFF22_11345"
     CDS             18823..19068
                     /locus_tag="VFF22_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17445.1"
                     /translation="MHDPILYSTDDGKLTLQLRACVWANQQAFAKRLISLPIRSTPYR
                     GAPSPATIKQSLTVQPVASRQISRSPQRDAALAVRQP"
     gene            19088..20602
                     /locus_tag="VFF22_11350"
     CDS             19088..20602
                     /locus_tag="VFF22_11350"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HZX17446.1"
                     /translation="MSHSPISQDEINAIIWKSCDTFRGSVDASDYKNYVLTMLFLKYL
                     SDVWQDHYEGYVAKYGNEPALIAAMMKTERFVLPASANFYSLHDKRHLPGNGERIDEA
                     LHAIEDANSAKLRGVFQDISFNANKLGEEKSKNNLLRHLLEDFAQADMNLRPSRIGSQ
                     DIIGNAYEFLISRFAAGAGKTAGEFYTPAQVSDLLAELLDPQPGEQICDPACGSASLL
                     MKCGRKVLEKTGSRNYALFGQESIGATWALAKMNLFLHGEENHDLRWGDSIRHPQLLD
                     ANGGLMHFDVVTANPPFSLEKWGVEQAENDKYGRFQRGIPPKTKGDYAFILHMIETLK
                     PGTGRMGVVVPHGVLFRGGSEGKIRQALIEENLLDAVIGLPEKLFFGTGIPAAILLFR
                     KTKSTQDVIFIDASRDFVAGKNQNQLGPQQLGRIVSAYRARQHQSRYAECVSLQAIRD
                     NDYNLNIPRYVDTFEAEDEIDLLAIKAEREGLERELVALKREMDGYLEELGYGA"
     gene            20592..21830
                     /locus_tag="VFF22_11355"
     CDS             20592..21830
                     /locus_tag="VFF22_11355"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HZX17447.1"
                     /translation="MVPKGWEVKSLGDIATISSGGTPSRSQSEYWDGDIPWVTTTEVN
                     FGTITNTAQKITQLGMQCSSAKLFPKNTILMAMYGQGKTRGQVAKLGIEATTNQACAA
                     IIPKSGHSHEFLFQYLQAEYENIRSMSNGGGQNNLSAGLIKEIPVPVPPLAEQTKIAQ
                     ILSTWDKAITTTERLLANSQQQKQALTWQLLTGKKRFADFSDTWGKQKLSEISTRIQE
                     RTDGEAHPILTISSLSGFVTQEERYSRYMAGESVNNYILLSRGEFAYNKGNSKTYQYG
                     CIFKLDTFDTGLVPHVYVCFKLNQGFDPEYYAQLFAADYLKDQLAGLVNTGVRNNGLL
                     NIKPSAFLDTTVPVPPLAEQQKIAQVLSTADAEISNLQAQLAKLKLEKKALMQQLLTG
                     QRRVRLDDEVEEEPQIRRVG"
     gene            22205..25480
                     /locus_tag="VFF22_11360"
     CDS             22205..25480
                     /locus_tag="VFF22_11360"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020795847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HZX17448.1"
                     /translation="MTTTPAFAHPKFQEEFSAKIPALALLANLGFTYLSPSQALALRG
                     SKERVVLPPVLRAELAKRRFFSNGVEHGLSEAALNKLVSEISHPPLNEGLQAANQRLS
                     DQLLYGVSVGDFVAGAKVNPTIALIDWGDLSNNSFHLTEELGVGCTSGIGQRIPDIVL
                     YVNGLPLVVIEAKRPDANNGKDLIQQGISQMLRNQRADEIPQLFAFSQLLLVVDGSGG
                     RYGTVGTTEKFWAAWREEEIGEVAFAELKNRPLDATQEQAIFAQRPAASLAWYRSWLA
                     GGLLASQQDKLLASLCRPDRLLELVRFYTYSDSKHGKIVARYQQVFGIRALIERIQQR
                     DVRGARQGGVVWHTTGSGKSITMLLLAKALIFHPQLQNCRVLVITDRTDLEAQLSKTF
                     ASAGVLTERDQQEALSQSGRHLAQQIGKGSVRVMFSLIQKFNSAAKLPECHNDSSDFI
                     VLVDEGHRSQGGENHVQMRLALPNAAFIAFTGTPLLLKEKTQSKFGPIVHAYTMQRAV
                     EDGTVTPLLYEERRLELDVNDRAIDSWFERSTRGLSEAQQADLKKKFAKKGELYKALG
                     RLELIAHDISEHFSSSVLDGLKGQVACDSRVSAIRLKEFLDEIGQVSSALVMSAPDSR
                     EGNSDSDEDSKSVVQAWWDKHVGKEDGTVYKQRMIDAFAQDDGPDLLIVIDQLLTGFD
                     EPRNAVLYIDKNLKQHNLIQAIARVNRLHPLKKHGLLIDYRGILGELDITIAQYQDLA
                     TRTQGGYSIEDLDGLYSPMSTEYKKLPSLYQQLWAMFKDVKNTQDIEQMLAVLLPRLQ
                     EVDGAQVDTRQKARDDFYALLSEFAACLAIALQSQAFFQDKSVSEDTRRQYKETVKQL
                     SNLRQRLKQATGDTVDYDEYAVQVKKLMDKHVLGIGVRESKGAYVVGDMGAHKPEAWS
                     EEKTRNEKDIIQTRLTKEIDQLLDDPYAQKRFSELLRQVIAEADALFDNPLKQYLLFK
                     SFDEQLQQRQLDELPDAVRGNPHVQAYFGAFKLLLDEQRVAAEAIDTPHWVLLAEQTD
                     AIVSQAVREFSINPLNIEAAVRQQLLPLYFMALKALGLGMDQVQALVEQVVQIVRAGA
                     RKA"
     gene            25480..26208
                     /locus_tag="VFF22_11365"
     CDS             25480..26208
                     /locus_tag="VFF22_11365"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001971014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HZX17449.1"
                     /translation="MASLHYGGETIAYQVQHVPRPARKLTIKVQPDGQVLVMAPADAS
                     AEQIAAGVNKRARWIWEQLQPIRERLLHVRPRQYISGETHFYLGKRYPLKVQEDARAP
                     QQVKLLRGMLCITVRQNRPEKVRELLFAWYKARAREQFNQRLERLTPQARWLTQKPAL
                     RLLTMQTQWGSCSHSGTLTLNPHLVKAPPECIDYVLLHELCHLAEHNHSEAFWRLLDQ
                     LMPDWQPIKARLDEMVELYLNDVR"
     gene            26459..27232
                     /locus_tag="VFF22_11370"
     CDS             26459..27232
                     /locus_tag="VFF22_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX17450.1"
                     /translation="MAEATPALEIRDLHKRYGDLEVLKGISLTARDGDVISILGSSGS
                     GKSTFLRCINLLENPHKGQIILAGEELKLKAAKNGDLVAADSRQINRLRSEIGFVFQN
                     FNLWPHMSVLDNIIEAPRRVLGQSKTEAIEVAEALLAKVGIADKRHVYPNQLSGGQQQ
                     RAAIARTLAMQPKVILFDEPTSALDPEMVQEVLNVIRALAEEGRTMLLVTHEMGFARQ
                     VSSEVVFLHQGLVEEQGSPEQVFDNPNSARCKQFMSSNH"
     gene            27252..28004
                     /locus_tag="VFF22_11375"
     CDS             27252..28004
                     /locus_tag="VFF22_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX17451.1"
                     /translation="MQNYKKILLAAAATLAFGTSAVAADKLKLGTEGAYPPFNLIDAS
                     GQVGGFDVEIGQALCAKMKAECEVVTSDWDGIIPALNAKKFDFLIASMSITEERKAAV
                     DFTEPYYTNKLQFIAPKGSDFKTDKASLKGKVIGAQRATIAGTWLEDNLDGVVDIKLY
                     DTQENAYLDLASGRVDGVLADTFVNWEWLKSDAGKSFEFKGDPVFDNDKIGIAVRKGD
                     ALREKLNAALAEIVADGTYKKINDKYFPFSIY"
     gene            28055..28750
                     /locus_tag="VFF22_11380"
     CDS             28055..28750
                     /locus_tag="VFF22_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012020052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX17452.1"
                     /translation="MNFDLYGFGPALAAGTLMTIKLALSALCLGLVLGLLGALAKTSP
                     YKPLQWLGCTYSTIVRGIPELLWVLLIYFGTVQLMRNLADLLGIESLELNAFAAGTIA
                     LGICFGAYATEVFRGAILAIPKGHREAGLALGMSKPRIFWRLILPQMWRIALPGLGNL
                     FMILMKDTALVSVIGLEEIMRRSQIAVTSSKEPFTFYMVAAFIYLGLTVVAMIGIHFL
                     EKRSSRGFVRSAS"
     gene            28747..29517
                     /locus_tag="VFF22_11385"
     CDS             28747..29517
                     /locus_tag="VFF22_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX17453.1"
                     /translation="MRKFSEIAAWSVAVALIAYALWGGLTALNWDVIIKWLPRLSEGA
                     LLTLELVAIAVLAGLILALPMGIARASRHWYVRALPYGYIFFFRGTPLLVQLFLVYYG
                     LAQFDAVREGPLWPYLRSPYWCAVLTMTMHTAAYIAEILRGAIQAVPPGEIEAARALG
                     MSRAKTMFYIVLPRAARIGLPAYSNEVILMLKASALASTVTLLELTGMARTIIARTYL
                     PVEIFFAAGMFYLLIAFLLVRGFKLLERWLRVDACQGR"
     gene            29554..30783
                     /locus_tag="VFF22_11390"
     CDS             29554..30783
                     /locus_tag="VFF22_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019093893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZX17454.1"
                     /translation="MPITTPTQVLQDAALLQRFQALDSFLIEHQALWRPRPFVHYPAL
                     PWEAEHPQLADWLRAQSLEHAEAAHNQPHLLQAPAPFPQLAAQAHYLSRTGELPQSPA
                     QVLPARFSADVPGRKWQQIDAFARSLSFIQKPQHWLDWCAGKGHLGRMLAHGGGNLTC
                     LEYDPLLVSNGQQLSNKLGITAQHIEQDVLADDAATQLQVQHTPVALHACGELHTRLL
                     QLASQAGCQQLAVAPCCYNRISTQRYQPLSASAQASTLQLSIDDLGLPLSETVTAGAR
                     VRRQRDQSMARRLAFDLLQRQLRGIDEYLPTPSLPAAWLDKPFADYCKDLAALKQLPT
                     PAPQDWQQLEQNGWQRLAEVRNLELLRGLFRRPLELWLLLDRALFLQEQGYDVRLGTF
                     CSHQQSPRNLLLLAERQ"
     gene            31077..31152
                     /locus_tag="VFF22_11395"
     tRNA            31077..31152
                     /locus_tag="VFF22_11395"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31110..31112,aa:Thr,seq:tgt)
     gene            32244..33350
                     /locus_tag="VFF22_11400"
     CDS             32244..33350
                     /locus_tag="VFF22_11400"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZX17455.1"
                     /translation="MSKLDTLQAFRCIGLTGVVIGHMTFAEEKYSGATLLPLAIKDFN
                     QLFIDWFFVVSGFIMVMVSRGRFQVPREILKFVYARLARIYPPYWVYFFITLAVFLAM
                     PEWVNSGYTEPNLWKSFLLIPDKTLPLVMVAWSLIFEMWFYLVFTSFMLCRERHLPWI
                     LAGWACLIICANLILELSSLSPALRIALHPFAIEFVMGGFIALFYFSPLSEHVSNRMA
                     VAMLLFVPLIGVPVGYSFGLFRGDGLIRVFSLGCLFGMLTIGATLLERREKLSVPSLM
                     VAIGDSSYTTYLAHLLVIGAVGKLWVLLGLSASNLINNLAFLVIMFLAVMIYGLVAFR
                     LIEEPLMNSANHLGKRLFRKKQQDTATLTAKTIK"
     gene            33347..34447
                     /locus_tag="VFF22_11405"
     CDS             33347..34447
                     /locus_tag="VFF22_11405"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZX17456.1"
                     /translation="MNSATMTRHSPRLRQNNFDLIRLLLALAVCLLHAHELSGVADLS
                     IIDTLLSGKIAIESFFVISGFLIFMSYEKSRSLADYLEKRARRIYPAYFASVLLAALL
                     LSSISERPLAAYFFSSDFIRYLASNLGFLNFIQPDLPGVFTENRLAAVNGALWTIKIE
                     VMFYLSVPIIVWLSRRVGRAPTLLALFVASTAFSLYMLHLHEITGRNLYVLLEKQLPG
                     QLRFFIAGAALYYLLPYFERHIKLLVLAAVLYFLAQSWVELEALKPVALAILVIFFAL
                     YNYAGNFCRFGDFSYGVYILHFPIVQTLVHFRAFEANPWLALLASVSLTLCAAALMWH
                     GLEKRWLLKNSHYVRASAQDEAPEKKREAHTL"
     gene            complement(34444..35853)
                     /locus_tag="VFF22_11410"
     CDS             complement(34444..35853)
                     /locus_tag="VFF22_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004882634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HZX17457.1"
                     /translation="MLSSTLMLTLATLIGLAAGFAREWLLIDAWGASLRSDAFLVALF
                     LPEAVRMALATGLFSSAALPLYQEREGAQREAWLSALTWRVLGVGLLLAGLFYLAAPQ
                     WVWLIGPGLSAEGQALAWQSLHLLAWCIPGFMLHALLCIPLQAHARFVLAGLGSLLFN
                     LPPVLYLALKGGQAEPAGVALSCLVGSLLMPLLLFPAMRGMGWRPWRVQADRSCGQQL
                     LQRLGPLLASTLASQGLALLERMIASLLGEGAVTWLNFARKLINLPMVALMSLNQVLL
                     GLMGGKSERERLVLLRRGTAMATLLTLPVAVGVVGAAEPLVLLLLPNQAGGGVLAALL
                     GWLSLTLLFAAWNALLARYAYSVGDTRLPLACELSGNAVNALLLLALPWWLGLKGIAL
                     AALCGVLLTGLLLMRKQRLLVALDWPRQWTLGGLLPALALAIPAMMVNSWWQLGVATL
                     AAMGLMAALAALLRPWRIH"
     gene            complement(35865..37274)
                     /locus_tag="VFF22_11415"
     CDS             complement(35865..37274)
                     /locus_tag="VFF22_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017906706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZX17458.1"
                     /translation="MTSSFGLPIALVLGLLFGVLLWFLSPLSVAVLIVAVVIGLNLLR
                     RPLLGLLLFAAVATFLPYSTINIGVRTTVSELLILVIWGCVLFQGLFTEPDHAQRSGP
                     TERYVLALILFSAFPFVVGQLMVQAEGNGVANWLRWMANMSLLFLVARLLRDGASRDW
                     MVLALMLGTLAMLALSLPIFLVERSALAINPLLTKLGYATLAEVIGDTTGAIFLRMGS
                     PWMHPNTLGGAMALVLPLAFCYGLTRHGWRRALAGAVGLLGVVALLLSGSRGALLSLA
                     VVLVWMAIRRVPFTGRLLLCGCGLGLLLVLSYPPLQERISTMFEFSDASTSVRFAEYA
                     NFSRAVASYPLGIGFKVEPPVPGTDLLGISNLWLNVIYKLGLPGLLLFIAATWSWWKE
                     TRPRTRNIDLNADNAIWLGTTAGLLAALFSGLFDHYFSFQPVLVGLFWLMIGLNLQEA
                     RRLREEAEVLPGAPAGNAP"
     gene            complement(37271..38371)
                     /locus_tag="VFF22_11420"
     CDS             complement(37271..38371)
                     /locus_tag="VFF22_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZX17459.1"
                     /translation="MRVGLDYRPGLSNPNNGIGRQNVALASVLGDLPGVELSLFTPAP
                     EDHPIRAQAHCPSWSSPIEGQHRLPRRLRFELAYMPWALRRLGIEVHISTFNMGLPLP
                     PRPPGTRYVLQLHDLFQLHLKNRHSSWLRETVYRLSDNLSIRYSIRVAERIWTPSQYS
                     ADEVARTFPWAASKVRVLPNLVPRCTLTPVAPPLPLPARFWLLVGTREPRKNVPWFLE
                     QWHLARAADAAVPELVLVGGGEFLPAALRALPGVHLMPALSDAELQGVYQAAERLWQP
                     SLAEGFGLPVIEALAGGTPVAVATGSSLDEIVAPGAPRFSPYDGAALVTLMHRLARPE
                     DPDRDALRAWAERYSEQPYRARVAELLQELQA"
     gene            complement(38362..39582)
                     /locus_tag="VFF22_11425"
     CDS             complement(38362..39582)
                     /locus_tag="VFF22_11425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003238572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZX17460.1"
                     /translation="MKILWVLPYLPWPTSSGGKTRQFNLLQALALAGHRITLLVQSKV
                     ALDDDSRQALEPLLERLIVLPRRPVRSPKTLLAIAASPLPMLASINGYAPDLSRRFTD
                     LLNETWDIVQIEHSYSFQPFERALQQAGQPFILTEHNVESALGGATYDRFPAWLKPLV
                     ILDQWRYRRWELRVMQQAEQVIAVTEQDAQALAQVLQKVLPVVVNGVDCARYATVMPA
                     YDSSRLLFIGNYEYPPNVDAVEWALDEVMPKVWAARPDVCMTIAGHALPPAWRHRWAD
                     TRIEWHGFVPDLTALQSRAALFFAPLRQGGGSKLKVLEAMAAGLAVVTTAQGVSGLAV
                     EAGVEYDGGDDAEALALAVLRQLASPLAAAQMGAAGRAYVNAEHDWMAVAEQLQRVYA
                     TCLAGRAEGDVACV"
     gene            complement(39592..40926)
                     /locus_tag="VFF22_11430"
     CDS             complement(39592..40926)
                     /locus_tag="VFF22_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-xylosidase"
                     /protein_id="HZX17461.1"
                     /translation="MRNRRFIGLATVVACVLLGAAVLSSRDAEAEITSLKPSRSVVWK
                     DYLGVNAQFHFFSEATYLKQMARLQELDLQWVRIAMHWALLERKPGQYFAPFDASVIA
                     MAEHGLKPVGFLAGSAPFATSAPASSPYQDSYPPTDNRLYSDSLSRFVERYPSVEAWQ
                     VWNEPNLPSFWRPKEDPDAYAALLLDAVDTVRQRFPDKPLGTAGMAYFSQMPSHNMEL
                     MLKALLTKGLAQKDLIVAYHPYTEYPEGNSPGNNELLDTVRFINSGLRGEGVKQIWAT
                     EWGWSSYSGPKEMQAIIGVSGQADYTLRRLALMSALDFDRIFLFNLSDLDARASVRDQ
                     SYGLLDLNADPKPAFRALKNFLKVTGPSLTPADPPSFGSAPKDLYSIAWTREDGRHLL
                     ILWSASAGKLTLPGIRQATLHDPHSGKARRLQAEAGALVVALKPSLQLLVWD"
     gene            complement(40916..42106)
                     /locus_tag="VFF22_11435"
     CDS             complement(40916..42106)
                     /locus_tag="VFF22_11435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011062487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZX17462.1"
                     /translation="MRIALLAPLPPEQTGIADYAEQLRVSLEAVGVEVVTPLAGCGND
                     PLCALERVRTFDWSGVALVHAELGGGRLAEFHALRALRERFAQLPLTATVHDPERLVW
                     RRASLPWPLSLTQRWPSPWPQAATVLADPLCLYEERRLANSLDALVTLTALGRNCLTR
                     RMRLPEQRVQVIAHGNQHIAAQPLPPLEPLRLLYFGFIYRGKGIEDLLDALALVIRQQ
                     PQLQQRVRLTLAGGSAPEMAFDAGESYIDQLRRRIVELGLESLIEWRLDLPSEVIAET
                     IQAHHVVVLPYRESKKLRLLGAQRGTSGALSWAAACGRGVITSDARAFAEEVSTGNGG
                     IFAQGDVAALASQLQSLLEQPERCQQWAERAAAIGQERLWPVTAQRFKALFTALCEGR
                     DYAQ"
     gene            complement(42106..44094)
                     /locus_tag="VFF22_11440"
     CDS             complement(42106..44094)
                     /locus_tag="VFF22_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012020065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide transport family protein"
                     /protein_id="HZX17463.1"
                     /translation="MIEIRTFRDLLRLFFIFFQEFKLAVIATLVVVVLGAFLLPTSYE
                     SNARLLVKPGRDTNTLPIEVADRQSIVMPATQRDPILDEERMLTGRPISRQVAEHYLE
                     ALANTPRPEGFFATLKYYVGQVVSGAVGLVRSVVQLLGLAEEQTPVDRLAARLEKNFS
                     VTHAPGSSVMEIRFRWSDPALAQAVVKSWVELYLEERTRALGRRSLYEFYLTQSEASA
                     ADILKYKQELGVRLNELGTVGIEAHLQNLSERINQLRGDRFNTWRLISSTEGSLATLE
                     QQIASLPAEVRTVREMALNPIHQDLVRMLNNKRAERQDLLRTFTENAPPVQSIDENIA
                     SLEAMVKAESATVQVSQNEAPNPIAERLRSNFMDQRADLARLKAQLLRQDEQLKLLEQ
                     DRGKALSVEPDLARLQRELTAAEKNFALYNESLEKARIDRELDNSRISNIAMIEQATY
                     NPSRVFPKSLVMLLLALPLSILVGFLVVYLCYLLDQRIHDGGRFASKFGIPLWTTLPE
                     ITAESSQSSAFLAALYRLYGMLPLEQVAERGLVLGLTSSRRGEGVSFIVGHLQLLLEE
                     RGHKVRIGPAPAEPGEVVLLDASALLSNPEAFVSLRQADLVALVVEAQQSNVPTVGHA
                     IEVLNTGFSKVDGVIINRRRFEVPDRVLRMMAKVRGVI"
     gene            complement(44113..44886)
                     /locus_tag="VFF22_11445"
     CDS             complement(44113..44886)
                     /locus_tag="VFF22_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011062485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HZX17464.1"
                     /translation="MKVFLLLVMTFGLVACTTPARIALPDSDTLEAGQVEALKLADLP
                     AVEERIYPGDTLRVVRASGETPTISAYTANSVYELTLYTVMNDGSFSYPFIGKVQAGG
                     KTPEQLSNELRERLTPYYREPGVTVNINQAPGNTVFVGGSVRNPQPYSTNVVTTLEQA
                     IVTAGGVLPDADSRHVALLRSDVDGRYKTYFVDYSQLLQGGEQGRQAVALQRGDVVFV
                     PKSGVGNRIEGVDLYLNQLIPFVKSIGIGASYDLRGNSN"
     gene            complement(44916..45827)
                     /locus_tag="VFF22_11450"
     CDS             complement(44916..45827)
                     /locus_tag="VFF22_11450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HZX17465.1"
                     /translation="MRLALIIPTRNAAAHLDRLLPALAAQTLQPDEFLVVDSASRDDS
                     VVRFRDAGARVEVIEPAQFNHGGTRRWASAQVDADVLIYLTQDAIPASPETFARIVSD
                     LQSEPDIGVAYGRQLPHPHAGLLGAQSRRFNYPPVSRTKRLRDASELGIKTCFSSDSF
                     AAYRREALCAVGGFPEDVIGSEDAYVAARLLLAGYAVRYAADAEVQHSHDYRVLEEFR
                     RYFDIGVFYGRESWIGQAFGSAGGEGKRYVLAEIAALREAGALHRLPEVLLRSAMKLL
                     GYRIGQGERFLDTGIKRRISMFPGYWK"
     gene            complement(45827..47284)
                     /locus_tag="VFF22_11455"
     CDS             complement(45827..47284)
                     /locus_tag="VFF22_11455"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017683163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HZX17466.1"
                     /translation="MSQLIPCIIAGGAGTRLWPVSREAMPKPFMRLPDGQSLLQKTFA
                     RVGQLPGVERVLTVTNRELLFRTLDDYRTVNQQDLPLEFLLEPFGRNTAPAIAVAALH
                     VQQQYGDHAHLLVLPADHLIRDEQAFAQAVASARELASQGYLVTFGIQPEHPETGFGY
                     IEQGQPLVAGAYAVSRFVEKPDLATAEAYVKSGQYLWNAGMFCLQVDTLLAQFQQFAP
                     DVLAAVQNCLARSPELSGNKLCQRELDSESFVLAPDISIDYALMERSDRVAVVPCNLG
                     WSDIGSWQALRELSPRDSEGNQIIGEAVLHDVHNCYIDSPRRLVGMVGLDNLIVVDTP
                     DALLIANAERSQEVKFIAQELKRRGHDAYRLHRTVTRPWGTYSVLEEGSRFKIKRIMV
                     RPGESLSLQMHHHRSEHWIVVSGMARVVNGEGDFLLNTNESTFIPAGHTHRLANPGVI
                     DLVMIEVQSGEYLGEDDIVRFNDIYGRVPAAEKAI"
     gene            complement(47296..48732)
                     /locus_tag="VFF22_11460"
     CDS             complement(47296..48732)
                     /locus_tag="VFF22_11460"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015636502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HZX17467.1"
                     /translation="MRKHQVDSLLLARPGFIEYFLAAIRIVHGATASIPGFILLWLLT
                     DSSVNLNHEYFGFLLFFGLLSVLMFQALGVYSEEIFSNRLRFRVMFFAWTAAFCLLLF
                     MHQALELFRYISLPFLVFWFSFSLAAFGVERLLLLVVFRYLIRRGHFLQTAVIVGGTE
                     NGVRLAEYLLRGQDIRSGLLGFIDDRIDRMPETLAGLPVIGNSRQLEKMIRDERVTQV
                     LVALPWSAENRIGQIVRDLRRLPVNVLLVPDMVAFRHAHNRITTVCGLPMFNVSKIPL
                     SGWSPLIKRLEDLMLAGLAIIALLPLMLLLAVVIKLDSPGPVLFRQNRYGYNNRLIEV
                     FKFRSMFYHQSDANAERQTTRNDDRVTRVGRFIRKTSLDELPQLFNVLLGSMSMVGPR
                     PHATATKAAGVLFEEAVEQYSARHRVKPGITGWAQINGYRGETDTLEKIEKRVEYDLE
                     YIESWSVWFDLYILFRTLPALILTEEAY"
     gene            49172..50224
                     /gene="rfbB"
                     /locus_tag="VFF22_11465"
     CDS             49172..50224
                     /gene="rfbB"
                     /locus_tag="VFF22_11465"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HZX17468.1"
                     /translation="MHILITGGAGFIGSALIRHLLNHTDYQVLNLDKLTYAGNLESLA
                     SVAGHPRYRFVQADIADSAAVSETLAEFQPDAIMHLAAESHVDRSIDGPAAFIQTNIV
                     GTYALLEATRSYWLQLEPARKAAFRFHHISTDEVYGDLHGVDDLFTETTPYAPSSPYS
                     ASKAASDHLVRAWQRTYGLPVLITNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYGN
                     GQQVRDWLYVEDHARALLKVVSEGKVGETYNIGGHNEQKNLHVVESICALLDELYPRQ
                     NGSYKEQISFVTDRPGHDLRYAIDASKIERELGWKPEETFASGLRKTVRWYLDNLDWC
                     RRVQDGSYQRERLGAL"
     gene            50236..51102
                     /gene="rfbA"
                     /locus_tag="VFF22_11470"
     CDS             50236..51102
                     /gene="rfbA"
                     /locus_tag="VFF22_11470"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HZX17469.1"
                     /translation="MKGIILAGGSGSRLHPITLGVSKQLLPIYDKPMIYYPISVLMLA
                     GVREILIISTPQDLPNFRKMLGDGSQFGVRFEYIEQPSPDGLAQAFILGEEFIGKDSV
                     CLILGDNIFHGQHFTEKLLRAAAEPSGATVFGYWVKDPQRFGVIEFDAQGKAISIEEK
                     PTVPKSSYAVTGLYFYDNDVIEIAKAVKPSARGELEITDVNNAYLQRGDLRVERFGRG
                     FAWLDTGTHDSLLEASQYVQTIEHRQGLKVACLEEIAYQQGWIDRQQLLAQAKAFGKT
                     GYGQYLFKLAEE"
     gene            51105..51650
                     /gene="rfbC"
                     /locus_tag="VFF22_11475"
     CDS             51105..51650
                     /gene="rfbC"
                     /locus_tag="VFF22_11475"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019094903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HZX17470.1"
                     /translation="MQAVSTDLPGVIILEPKVFGDQRGFFFESFNARAFAEATGLQRE
                     FVQDNHSRSAKGVLRGLHYQVQQAQGKLVRVTAGEVYDVAVDLRRSSPTFGKWVGTRL
                     SAENNRQLWIPEGFAHGFVVLSEFAEFLYKTTDYYAPEHERCIRWDDPTLAIDWPLEN
                     APQLSAKDQAGLSFADAQVFP"
     gene            51647..52513
                     /gene="rfbD"
                     /locus_tag="VFF22_11480"
     CDS             51647..52513
                     /gene="rfbD"
                     /locus_tag="VFF22_11480"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003187713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HZX17471.1"
                     /translation="MKILLLGQHGQVSQELQRSLNGQAELTVLSREQLDLAQPELIRQ
                     HVQRLRPELIINAAAHTAVDQAESEPELAFTINATAPGILAELAAELGAPLIHYSTDY
                     VFDGSHTEPYRESDATHPLSVYGRSKLAGEQAIQAVGGMHLILRTSWVYSQHGKNFLL
                     TMQRLLQERESLSVVDDQIGAPTWAGTIAQATAQLIEQWRSGHAGPWGVYHLTAQGET
                     SWFGFASAIAEHLRSQGKSVATLQPIPSSAYPTPAQRPLNSRLDCSRLQQDWHVQLPD
                     WQAALAQCLARS"
     gene            complement(52510..54135)
                     /locus_tag="VFF22_11485"
     CDS             complement(52510..54135)
                     /locus_tag="VFF22_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX17472.1"
                     /translation="MLAQLTVRARLLLLAVVPLLVLIVVIGMALSNASKLNNSFDELF
                     TDRMQPISQLKVVSDAYAVAMVDALHKYRAGLFDESTLRKAFSEARSRGDKAWAEYIA
                     TRLTSDESNRVERTKPHLLRVQQLADQYLAQLGNGQLLAAEAGPFNRILYDTFDPLGG
                     ELEGLISLQLSEGERLNAETTAQYQSMRTTFLLIGAVALVLVLVAALLISLSIIRPLA
                     GLRSLISEVQQSSNLTLRADASGRDEVSDTARAFNIMLEHQQALIRHLTDTATQLAAA
                     SEEMSAISAQVSQAATAQGDQTSMVATAVHQMSVAVQEVARNAQSTASNAADANKEAR
                     QGSELVQSNLKSIQGLSASVEKAGEVIDTLHSQSDEISKVLGVIQSIAEQTNLLALNA
                     AIEAARAGEAGRGFAVVADEVRSLASNTQKATESIRSMIDALQGGARSAVSAMQLSRE
                     QAQNSVSHAREAGEVLNHIAHAIEGIADGNVQISAATEEQTAVANEISQNISSLNDSI
                     GEVVNGAEQSSIASRDLAQLASGLQQQIQRFRA"
     gene            54304..56118
                     /locus_tag="VFF22_11490"
     CDS             54304..56118
                     /locus_tag="VFF22_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX17473.1"
                     /translation="MPTAHPHPRRPRWRSLALLALLLTPLLWPLQQLAERYYSGVLVE
                     QNRQTLDLYVANLLGTLRRYEVLPPILGDLPALRLALQQPSERALLENANRLLDQVRG
                     QTGADVIYLMDPNGKTLAASNWAQADSFVDRNFAFRPYFREAIAGRPGRFFGLGTTSG
                     KRGYYFAAAVRDGERSLGALVVKVDLDHTETLWGNTPEQLLVTDANGVVILTSNSQWR
                     FTASRPLDAAERAAIAANLPYPTQAPPPIKLDAQNWIRQDRELKETGWTVSILAPRSL
                     LDRPVRTALVAGGGMLLLLMLLLGLLMQRRRHYLERIALNTQARQQLEQRVLERTLDL
                     ERLNSRLKQEVLEREQAQQELVQAQDELVQASKLSALGTMSASISHELNQPLAAIRSY
                     ADNACVLLDHQRGDDARGNLKLISQLTERMASIIAHLRAFARRDRHAPESVALQPAID
                     DALALLAKRRQAMQVELIRDLPNATLWVQAGETRLRQVLGNLLANALDALSEVPQPRR
                     LWLSAELTADGVDLHLRDNGPGFSQEALQRAREPFFTTKTSAQGLGLGLAICDTLMRA
                     LGGELLFANHPDGGAQLTLRLRAADQGIKTQSPEDFVV"
     gene            56115..57509
                     /locus_tag="VFF22_11495"
     CDS             56115..57509
                     /locus_tag="VFF22_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZX17474.1"
                     /translation="MSMIDSRTQVLLIDDDPHLRQALSQTLDLAGLKVSSLADAHGVA
                     ARIERDWPGVVVSDIRMPGIDGLQLLQQLQEQDPELPVLLITGHGDVPLAVQAMRAGA
                     YDFLEKPFASDDLLDSVRRALALRRLVLDNRSLRMALADRHTLSARLVGQSPAMLRLR
                     EQIGALAGINSDVLILGETGAGKEVVARALHDLSSRRAGPFVAINAGALAESVVESEL
                     FGHEQGAFTGAQKRRIGKFEFANGGTLFLDEIESMSLEVQVKLLRLLQERVVERLGGN
                     QLIPLDIRVIAATKEDLRQAADQGRFRADLYYRLNVAPLRIPALRERGEDALLLFKHF
                     AEAASSRHGLAERSLQPGQRAALLRHPWPGNVRELQNAAERFALGLELDLDLQAQAAA
                     PMPDEDGNSGGLNAQVEAFERSLISAELSRSHSSLRSLAEALGVPRKTLHDKLRKHGL
                     SFADAGGSSPDSLD"
     gene            57804..58793
                     /gene="dctP"
                     /locus_tag="VFF22_11500"
     CDS             57804..58793
                     /gene="dctP"
                     /locus_tag="VFF22_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP substrate-binding protein
                     DctP"
                     /protein_id="HZX17475.1"
                     /translation="MFKLTAKALACALSLSIAGMAQAADPIVIKFSHVVADHTPKGQG
                     AALFKKLAEERLGDKVKVEVYPNSSLFGDGKEMEALLLGDVQLIAPSLAKFEQYTKQI
                     QVFDLPFLFKDMAAVDRFQQSPEGQALLKSMEEKNITGLGYWHNGLKQLSANKALREP
                     KDARGLKFRVQASAVLEEQFKAVKANPRKMSFAEVYQGLQTGVVNGAENPYSNIYSQK
                     MHEVQKFITESNHGVLDYMLITNTKFWNSLPADVRSELDKIIVEVTAEVNKQAGALND
                     GDKQRIIDAKTTEIITLTPEQRNEWRDAMKPVWSKFEAEIGADLIKAADAANQ"
     gene            58921..>59021
                     /locus_tag="VFF22_11505"
     CDS             58921..>59021
                     /locus_tag="VFF22_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HZX17476.1"
                     /translation="MNALRRVWDHFEEGFIAFLLAAMTLVTFVYVIL"
BASE COUNT        12835 a        18993 c        16554 g        10639 t
ORIGIN      
        1 cgaaaaccag cgttatgacg tgatagtggt caactacgcc aacggcgata tggtcggcca
       61 taccggcgtg ttcagcgccg cggtcaaagc agtggaatgc ctggacagct gcgtcggccg
      121 tatcgtcgag gcgctggaca aggtcggtgg cgaggcgctg atcaccgccg accacggcaa
      181 cgtcgagcag atggaagacg acagcaccgg ccaggcccac accgctcaca cctgtgagcc
      241 ggtgccgttt atctatgtcg gcaagcgccc cgcgcacatc cgcgaaggcg gcgtcctggc
      301 cgacgtggct ccgaccctgc tgaccctgat gggcctgccg attccaccac agatgaccgg
      361 caccagcatt atcgagctgg gctgaaggcg aaaaagtcac tcccccacag acgtagccat
      421 gctcgccagg ctctgtagcc cggataagcc ttggcgcaat ccgggatttc ggccccggat
      481 tgcgctgcgc ttatccaggc tacgggattg ctcgcaacca agctcctgcg cgggcatgcg
      541 ccgccccggc cattaacgca cacgccggta cttacgactt gcagcttact gctggttttt
      601 tatgctcagc acatccatgg cggcgaccct gcgggccgtc gctgtgcgac gttaaaaatg
      661 gctcccggcc attttttagg catactaggc gcgttcttaa ttcaggtact cccagcccca
      721 tgtttcgtgc cctcgccgtt attttcctga ccgtcctgct gacgcctgcc atcgccgacg
      781 agaagagcga tgcgcagaaa caactggacg ccgcccgcgc cgatgtcagc gaactgaaac
      841 aactgctgga gaagctgcag caggagaaat ccggtgtgca gaaggacctg aagaaaaccg
      901 aaagcgagat gggccagctg gagaagcagg tcaaaggcct gcagcaggac ctgaaaaaga
      961 gcgaagagga aatccggcgc cttgatagcg agaaaaaaaa acttgagggc gcgcgccttg
     1021 agcaacagcg cctaatcggc atccaggccc gtgccgccta tcagagcggc cgccaggaat
     1081 acgtaaagct gctgctcaac cagcagaacc cggaaaaatt ttcgcgcacc ctgacctact
     1141 acgactacct cagcgaagcg cgcatggagc aactcgccgc cttcaatgaa actctgcgcc
     1201 agttggctaa tgtggagctg gatatcgcca accagcaacg ccagttgctg gagcagaaaa
     1261 gcaccctgga cagccgccgc gaacagctcg ccagcgtgcg caaggagcgt cagttggccc
     1321 tggccaagct gaacaaggaa ttctccgccc gcgaccagaa gctcaaggcc cgccagcagg
     1381 agcaagccaa tctggcgcgc gtgctgaaaa ccatcgagga aaccctcgcc cgccaggccc
     1441 gcgaagctga ggccgcgcgc cagcaagccc tgctcgccga acgcgaacag ccgcgcagca
     1501 gcactaatcc cgcgccgacg ggtccattgg tgagctccgg cgccagtttt ggcggcccat
     1561 tcgccaaggc ccgcggcaaa ttgccctggc cggtggatgg tcgcctggtg gctcgctacg
     1621 gcacgccacg cggcggtgac gcccgcacca agtgggatgg cgtattgatc ggcgcttccg
     1681 ccggcagtca ggtgcacgca gtgcatggcg ggcgcgtggt gtttgccgac tggttgcgtg
     1741 gcgctgggct tctagtcatt ctcgaccatg gcaacggcta cctgagcctt tatgggcata
     1801 accagagtct gctcaaggat gccggcgacg tggtcaaagc cggcgaagcc attgccaccg
     1861 ttggcaacag cggcggccag gacaccccag cactgtattt cgcaattcgc cagcagggcc
     1921 gcccgatcga ccccgctcaa tggtgccgcg cacaaggata agtgctgcga ttaacgattc
     1981 aggatcagga gtaagttcga catgccgcat ctgttccgcc tcaccaccct cgcactggcc
     2041 attactctgc tgggcagcac cgccgtattt gccgcacccg ccgaagcacc ggccaccgtc
     2101 aacgacagcg cgccactgcc gctggacgat ctgcgcacct ttgccgaggt gatggaccgc
     2161 atcaaatccg cctatgtcga gccggtcagc gacaagaccc tgctggaaaa cgcgatcaag
     2221 ggcatgctca gcaaccttga cccgcactcg gcctacctgg agccggaagc ctttcgcgac
     2281 ctgcaggaaa gcaccagcgg cgaattcggc ggcctgggta ttgagctcgg cagcgaggac
     2341 ggctttctca aggtgatctc accgattgat gacactccag cgtccgccgc tggcatccag
     2401 ccgggcgacc tgatcgtgaa gatcgacggc cagccaacca agggcttgtc gttgatggaa
     2461 gccgtcgaga agatgcgcgg taaagccggc agcgatatcg aactgaccct ggtgcgtgaa
     2521 ggcggcaagc ccttcgatgt cgaactgacg cgcgcggtaa tcaaggtcaa gagcgtgaaa
     2581 agccagctgc tcgatgacgg ctacggctac gtgcgcatca cccagttcca ggtcaacagc
     2641 ggcgaagaag tcggcaaggc cctagccaag ctgcgcaagg aaaacggcaa gaagctacgc
     2701 ggccttgttc tcgatctgcg caacaacccc ggcggcgtgt tgcaggcagc agtggaggtc
     2761 accgaccact tcctgaagaa gggcctgatc gtctacaccg aggggcgcat tgccaactcc
     2821 gagctgcgct tcaatgccga cccggccgac gccagcgaag gcgtaccgct ggtggtgctg
     2881 atcaacggcg gcagcgcctc ggcctccgag atcgtcgccg gcgccctgca ggatcacaaa
     2941 cgcggcgtac tgatgggcac cgatagcttc ggcaaaggct cagtgcagac cgtgctaccg
     3001 ctgaacaacg accgcgccct gaaactcacc accgcgctgt acttcacccc caacggccgc
     3061 tcaattcagg cccagggcat cgttccggat atcgaagtgg cgcgcgccaa actgacccgc
     3121 gagcagggcg acgagaccat caaggaagcc gacctgcaag gccacctggg caatggcaac
     3181 ggtggcgccg acaagccaag caagagcaaa accggcaccc cagcacgccc gcaggatgac
     3241 gactaccagc tgagccaggc actcaacctg ctcaagggtc taagcgtcac ccgcggtgac
     3301 tagaagtacg tttgtaggag cggctgggcg gcattccgcc atgcccgcga aactgcagcc
     3361 aagaaaaagc ccgaccttga tcactcaggg tcgggctttt taataacgcc tgaaattaca
     3421 ggtagttatt ggtcatctca tcaacaaagc cttcagcacg cagctcgtca agggccttct
     3481 gcagacgctc taccacctca tccggggtgt ctttattcag cgccagatac agctcggcct
     3541 cattaaagcg cagcgcggta ttcaggccgc tgacaccttc ctgcttggcc aggtagcggc
     3601 cgaccgggtc agtggtggcc cacaggtcga tcttgccgct gagcagcttc ttgatgttct
     3661 cctgatcgcg cagcgcattg atcgggctca ggccctgact ttccagatgc tggctgaccg
     3721 cgtcgttctt gtaagcgccg atctggtact gcgcggcgtc cttgaggctg gcaacgctca
     3781 agttattgcc cggagcagcc agcagtaccc aaccggtttt cgccaatggc cctacccact
     3841 tgaacagtgg ttggcgctca ggggtgaagg tggtggagaa caggccgtag ttgggcttat
     3901 ccagggtcag gcgatacagg cgatcccaag ggaagcgcag ggtcaggctg tagttgatgc
     3961 cggcacgctt gaacatctca cggacgattt cggcactaat gccatcaata ccgtcgtcac
     4021 gggcgtagtt cttgtcatcc acagccatgt tgaatggcgg gaagttctcg gtgagcaaca
     4081 ccactttgta atcggctgga agctctgcac gggctgcgca ggcactcagg acaagcccga
     4141 acaacaggct ggctttcagg gttttcaaca taggtcattc cgctcgcatc aattgtgatt
     4201 atggctagcg gcatccctgc tcgggctagt gcccggcgac gtatggtacg tggcgtcaac
     4261 catccatgga cgcagaccgg cttgtggcca gcctgctggt ttcgcgtgtt acaggtagtt
     4321 gttcaaggtg tcgtcgacaa aaccttcgct gcgcatcttg tccagctcgg cttgcagctt
     4381 ctgtacaact tcgtccggca cctctttgtt cagcgccaga tacagctctg cgctatcgaa
     4441 acgcagaatg gttttcagcc cagaaacacc ttcctgcttg gctaggtagc ggccggcagg
     4501 gtcaccagta gcccataggt cgatctgacc ggccatcagc ttcttggcgt tttccgtatc
     4561 gcgcagggcg gtaaccggct ccaggccttt ctccaccaga tgctcggcaa tcgcatcgcc
     4621 cttgtaggca ccgaccttgt actgcttggc gtcttccagg ctgttcagag taatactgct
     4681 gtcggccttg ccgagcaaca cccagtcatc cggaccaatc gggccgaccc acttgaacag
     4741 cgcttcacgc tcgggcaaac gcgctgcaac aaacacgcca tagcctggct tttccagcgc
     4801 cagcttgtag atgcgatccc aggggaaacg caaagtcatg ctgtactgga taccagcacg
     4861 cttgaacatc tcgcggatga tatccacggc aatgccgtcg atattgtctt catgagcgaa
     4921 gttcttgccg ttgatcgcca tgttgtaagg cgggaagttc tcggtcagca gcaccaggct
     4981 gtagtttggg tcgacttcag cccgggcact gccagagagc accataagag tgccagtgag
     5041 ggcgattagc aattgtttaa acatgtgatc tcacgtcgat agtggcggct gtacgtgcaa
     5101 gcatacccag cactgttggc ttcgcccagc caggatacaa gacgctggac tgaccgcatg
     5161 aaccttgagc aagcaaaccc ttggggggct ttgtgcattg caacaagacc cgcaacttat
     5221 gcaccaaagc ggggaaagct ctgttgcttc agcaataggc aacgattcgg attctagcga
     5281 agcagtcgac attttgctgt cgactcgccc gcagcagtgg ccggcagggc tgccggccag
     5341 cgaagtttag cgcacaacaa ttccgcgagc ggccatatac gccttagctt ccggcacggt
     5401 gtattcaccg aagtggaaga tgctggcggc cagcacagca gctgccttgc cttcaataat
     5461 gccgtcagcc agatgctgca ggttgccgac gccgccggag gcgattaccg ggatgccgac
     5521 catttcgctg atcgcgcggg tcacgcccag gtcgtaaccg ctcttcacgc cgtcctgatc
     5581 catgctggtc agcagaattt cgccggcgcc gaggtcttcc atctttttcg cccagagcac
     5641 cgcatccagg ccggttggct tgcgcccgcc gtgagtgaag atttcccagc gcggagtttc
     5701 gcccggcttg gagactttct tcgcgtcgat ggccacgacg atgcactgcg aaccgaaacg
     5761 tgaggcggct tcaccgacaa actccggggt aaacaccgca gcagtgttga tcgagacctt
     5821 gtccgcgccg gcattcagca ggttgcggat gtcctgcacg gcgcgcacgc cgccgccgac
     5881 ggtcagcggg ataaacacct ggctggccat acgctcgaca gtgtgcaggg tggtatcgcg
     5941 gccgtcgacg ctggcggtga tgtcgagaaa ggtaatctcg tcggcgccct gctcgtcgta
     6001 gcgacgggcg atttccaccg ggtcgccggc gtcgcggata ttctcgaact gcacgccctt
     6061 gaccacgcgg ccgttgtcga cgtcgaggca ggggatgatg cgtttggcga gggccatggc
     6121 aatttctctt gtagggtgcg ccgcgcgcac cgaaaaccgt cacgggcgcg cctggcgcac
     6181 cctacctcag ctgtttgcgg ctggtttatt cgtctttgta tttcaggtca caaagcgcct
     6241 gggcttcggc cacatccagg gtgccttcgt agatcgcccg cccggtgatg gcgccgataa
     6301 tgcccggcgt gttggtgtcc agcagcgcct gaatatcgcc gatattgtgg atgccaccgg
     6361 aggcgatcac cgggatgcgc gtggcgttgg ccagggccac ggtgtaagga atgttgcagc
     6421 cctgcatcat gccgtctttg gcgatgtcgg tataaacgat ggcggacaca ccgtcggctt
     6481 cgaactgctt ggccagatcg atgacttgca cggtgctgat ttcagcccag ccgtcagtgg
     6541 cgacaaaacc atctttggcg tcgaggccga cgataatttt gcccggaaac gccttgcacg
     6601 cctcggcgac gaaggcagga tctttcaccg ccttggtgcc gatgatcacg tagctgaccc
     6661 cggctttgac gtaatgctcg atggtttcca gcgaacggat gccgccgccg atctggatcg
     6721 gcaagtgcgg ataacgcttg gcgatggcgg tgaccacttc gccgttgacc ggctggcctt
     6781 cgaaggcgcc gttgaggtcg accagatgca gacggcggca gccgccttcg acccacttgg
     6841 cggccatgct caccgggtca tcggagaaca ccgtggaatc ttccatgcgg ccctggcgca
     6901 ggcgcacgca agcgccgtcc ttcagatcga tagcggggat aatcagcatc ggttgaacct
     6961 gctcaagtcg tgtgaattca gtgggtcagc tcttttccag cgcccagagg tcgctttcaa
     7021 tgctctcgaa ccgttctttc aggtgagtct gcacatcgaa aatcgctcga ttgtagtagt
     7081 gcggcgcaat ctcgcgggca aacagctcca gcacttcagc ggcctcgaag ctgcccagct
     7141 ccaactcgaa gcgctcggcc ataaaacgct tgagcaccag gcaggcttca cgctcctgct
     7201 cggcgttcaa ggtcatcagc ggcggcttgt tcttggcccg gctcatttac caacggccat
     7261 cccaggcggc gaagttctgc agcaactgca ggccgtgggt atggcttttc tccgggtgga
     7321 actgcacggc aaagcgcgag ccgtcggcca gcgctgcagc gaaatccttg ccgtagtggc
     7381 cgcgaccgac cacctgctta gagttgccgg cctcgatgta atagctgtgc acgaagtaga
     7441 agcgcgccag gtccgggatg ttgtgccaca gcgggtgctt gaccgactgc gccacttcat
     7501 tccagcccat atgcggcacc ttcagcggct cgccgtcttc gaccataccc ttgccgaaga
     7561 agcgcacctt gccggggaac agaccgatgc aatcgacgcc atcgttctct tcgctgtgct
     7621 ccagtaacgc ttgcataccc acgcagatgc cgagaaacgg ccgatcggcg ctgacttcac
     7681 gcaccaggct gtcgaagccc aggcgcttga tctcggccat gcaatcgcgg atcgcgccga
     7741 cgccggggaa tactacgcgg tcggcttcgc gaatcacctg ggcatcgctg gtcaccagca
     7801 ccttgccggc gcctacgtgc tccagggcct tggccactga atgcaggttg cccatgccgt
     7861 agtcgattac cgcaacggtc tgcatttata gacagccctt ggtcgacggc atctgcccag
     7921 ccatgcgcgg gtccagctcg acggccatgc gcagggcgcg accgaaagcc ttgaacaccg
     7981 tttcgatctg gtgatgggtg ttggtgccac gcaggttgtc gatgtgcagc gacaccagcg
     8041 cgtggttgac gaagccctgg aagaactcct ggaacaagtc cacgtcgaag ccaccgacca
     8101 ccgcacgggt aaacggcacg tgcatctgca ggcccggacg accggagaag tcgatcacca
     8161 cgcgcgacag cgcttcatcc agcggcacgt aggaatggcc gtaacgggtc atgcccttct
     8221 tgtcgccgat ggctttggta aaggcctggc cgagggtgat accgacgtct tccacggtgt
     8281 ggtgatcgtc gatatgcaaa tcgcccttgc actcgatatc gaggtcgatc aaaccatggc
     8341 gggcgatctg gtcgagcatg tgctcgagga atggcacgcc aatatcgaac ttggccttgc
     8401 cagtcccatc caggttgatc gagaccttga tctgggtctc cagggtattg cgctcgacgg
     8461 atgccgtacg ttcggccatc accagctcca gaaattgctc gcggaaaaag gctgccatta
     8521 taggggcaaa ggcgtcgggg ggatatgcac cccgccggct ggactgcatc ttgcagacgt
     8581 ccggatattt accatcccga ggcggcgatc atgcatgagc ggaaaatgcc ggccaccctg
     8641 cgctttatgc tggcggctcg gcgcagcgag ttactcggcc tggaagacct gtcgcagacc
     8701 tgcgagttgg tcacccgcat cagccagctg gtgcatgccc tgcaaaaaga gcgcggctat
     8761 tccaacctct acctgagcgg caacgccccg caccagcgcc tgcaactgga cggcctgagc
     8821 caggccgccg acagcctgga gcgccaggca cgcgacgacc tggaacgcat cgaccagaac
     8881 ggccttggct cggccgacaa gacccgcctg ttcacccgca ttgcctatgc cctgcacagc
     8941 ctcgacgagc tgccagcgct gcggcgcaag attcgcgagc accgcatcag catgcaggac
     9001 gccaccgccg ccctggtacg gctgatcggc ggcctgctgg cagtagtctt cgaggccgcc
     9061 gacacggcca tcgaccccgc catcacccgc agcctggtgg cgctgttcaa ttttatgcag
     9121 ggcaaggagc tcgcaggcca agagcgcgcc ctcggcgttt ccgggttcgc cagcggctac
     9181 ttcaccgccg agatgctcga acggctggaa cacctgctgg aatgccagca acgcagtttc
     9241 gcgaccttca gccgcttcgc cagcccggca gccctggagc tctggcagag cctctgcgcc
     9301 agccccagca acgctcaggc ctgccgtcta cgcgagattg cccggcacac ccacgccgac
     9361 gcacgggtcg actgccagtt gggcgagctc tggttcgaac tgcacaccgc gcgcatcgac
     9421 gcgatgaaaa gcgtcgaaac ctgcctagag caggacttac tgcaacgctg ccaacaaagc
     9481 atgcagcgcg tccgccgtga cctgcacagc catcgccgtc tgcttgatgg cctcagcgac
     9541 atgcacgccg ccgccgagca ggccaggcta ttcagcgtgc acaacagcga ctgcgacagc
     9601 ctgaccgagg aaagcccaag cgccaacctg gcgcgttcga ccctggattt gctgcaaagc
     9661 cagacccagc gcctgcaggg cctgcatgac gaactgcgcg aagcgcgcca ggccctggac
     9721 gagcgcaagc tggtggaacg cgccaaacaa ctgctgatca agcaacacgg ctgcagcgag
     9781 agcgaagcct atgcccagtt gcgccaggca gcgatgaacc aaagcctgcg cctggatgaa
     9841 gtggctcagc gcctgctggc tcgttctatc gaagccccca tgaccagccc acgacaaaag
     9901 cactgaactc aagcacgcgc cgccccacac gcaccgcgac cgtgcaccac cgcgacggct
     9961 tagcgcagcc ttcggcccgt gcaaacggac gccgcactcg cccggccggg caacgcaagc
    10021 cattgatctg ataggcctga ttaccctgag gaaggcatcc gcgaaggtat tacgcacatc
    10081 ctggcacggc tttcgctatg gctagaccaa gcctggcagc aatgccaagc gccacaactc
    10141 aacccggaca acgatgtcca cgccccggct aagccggcca gcgtgacatc gttttttttc
    10201 gcccagagga aaccgagcca tgaccgacaa gccgagcaat gacgacagca acagcgtact
    10261 gatggcctcg cgccggcgct tcctgaaaca gtcgatagtc gccgccagcg gcattgctct
    10321 gttcggcggc atgggcatta gcagccagag cagcgcctgg gccgcaggtg gcgaggtgga
    10381 aaccaccaaa gccaaactcg gctttatcgc cctgaccgat gccgcgccac tattcgtcgc
    10441 cgccgagaag ggcttcttcg ccaaatacgg catgaccgag gtggaggtgc tcaagcaatc
    10501 ctcctggggc accacccgcg acaatctggt gctgggctcg gcgaaaaacg gcatcgacgg
    10561 cgcacacatc ctcaccccca tgccctacct gatcagcgcc ggcatcgtca ccccgaacaa
    10621 ccaaccggtg ccgatttcca tcctcgcccg cctcaacctg gccggccagt gcatctcggt
    10681 gggtgaggaa taccgcgacc tgaagatcgg cgtcgacagc accccgttca aggcagcgct
    10741 ggaagcgaag aaggccagcg gcaagaaagt cagcgcggcc atgaccttcc ccggcggcac
    10801 ccacgatttg tggatgcgct actggctggc cgccggcggt atcgacccga acaacgacat
    10861 cagcaccatc accgtgccgc cagcgcagat ggtcgccaat atgaaggtcg gcagcatgga
    10921 taccttctgc gtctgcgagc cgtggaacgc ccagctgatc aaccagaaga tcggctacac
    10981 ggccaacacc accggcgagc tgtggcacaa ccacccggaa aaatccttcg ccctgcgcag
    11041 cgattacgta gccgccaacc ccaaggccgc ccaggccctg accatggcga tcatggaagc
    11101 gcagatgttc tgcgaactgc cggaaaacaa ggaagaagta gcgaagatct gctcgcagcg
    11161 ccgctggatc ggcgcgcctt acgccgacat cgtcgaccgc atgaagggca acttcgccta
    11221 cggcaccggc aaagtggtgg aaaaccatcc cgagcagatg cgctactggg acgacttcgc
    11281 ctcctaccct ttccagagcc acgacctctg gttcctcacc gagaaccagc gctggggata
    11341 cctgccgcgc gacttcgaca gccaggcact gatcgcccag gtcaaccgcg aggacatctg
    11401 gcgcgctgcc gccgccgccc tgagcctgcc ggccgagcag ataccaacct ccacctcgcg
    11461 cggcatcgag aaattcttcg acggcaaggt gttcgacccc gagaacccgc aagcctacct
    11521 ggacagcctg accatcaagg ccatggcctg atcgccatgc accgcctgcg aggaaatacc
    11581 cgatgaacgc caacgtcaaa atcgagcccg ccgccgtggc cagcccggct acaccagcca
    11641 caccctcgcg gcttagccgt atcggcacaa gcctgcagca gaccctgctg caggatttac
    11701 tgccaccgct ggtgatcctg cttatcctcg gcctggtctg ggagatgctc tgttcgggcg
    11761 ctggcgcggc cttgccgccg ccgtcccagg tgatcagcga gacctgggag ctgatcatcg
    11821 accccttcta tgacaacggc ggcaacgatg tcggcctggc ctggcaactg ctggccagcc
    11881 tgcaacgggt ggcggtgggc tacctgctgg ccgtggcggc aggcgtcgct ctgggtgtac
    11941 tgattggcca atcggactgg gccatgcgcg gcctcgaccc gctgttccag gtgctgcgca
    12001 ccgtgccgcc gctggcctgg ctgccgctgt ccctggccgg ctttcaggac agccatccgt
    12061 cggcgatctt cgtgatcttt atcaccgcga tctggccgat catcatcaac acctcggtgg
    12121 gcatccgcaa tatccctcag gactaccgca atgtcgccca ggtgatccag ctcaacgcct
    12181 gggagtactt ccacctgatc atgctgcccg cagcagcgcc ctatatcttc accggcctgc
    12241 gcatcggcgt cggcctgtcc tggctggcga tcatcgcggc tgagatgctc atcggcggag
    12301 tcggcatcgg tttctttatc tgggatgcct ggaacgcctc gcgcatcagc gacatcattc
    12361 tcgcgctcgt ctacatcggc atcgtcggct tctgcctgga tcgcctggtg gccttcgttg
    12421 gccagcgcat cacccgcggc attcccgctg cctgaggatt cggacatgag ctatctcagc
    12481 gttgaacaac tggacatgag ctttaaccgc ggcaaggtcg cctctcaggt gctcaagggc
    12541 atcaacctgc gcatcgacca gggcgaattt atctcgatca tcggccactc cggctgtggc
    12601 aaatccacag tcctgaacat cattgccggc ctgctggaac ccagcctcgg cgtggtgatt
    12661 ctcgacggca aggaggtcaa cggccccggc ccggatcgcg gcatggtgtt ccagaaccac
    12721 tcgctgctgc cctggctgac ggtgtttgag aacgtcgccg tgggggtcaa caaggtgttc
    12781 aagcgcagca tgagcaaggc agagcggcgc gaatgggtgg agcacaacct gaaactggtg
    12841 aatatgggcc acgccctgca caagtacccc caggagatct ccggcggcat gaagcagcgc
    12901 gtcggcatcg cccgcgccct ggcgatgaag cccaaggtac tgctgctcga tgaacccttc
    12961 ggcgccctcg acgccctcac acgcgcccac ctgcaggacg aggtgatgcg tatccaggca
    13021 gacctgggca acaccatgat gatgatcacc cacgatgtcg acgaggcggt gctgctgtcg
    13081 gatcgcatcg tgatgatgac caacggcccc tcggccacca tcggtgaaat cctcagcgtc
    13141 gacctgccgc gcccgcgcga ccgcatcgcc ctggccgacg accacgaata caaccgctgc
    13201 cgccgcgcgg tactgagctt tttgcacgac cgccatcggt tgcagtaagc gacagctttc
    13261 gcgaaagaat tatcaaggct ccgtctgaac tggctgctgt tggcaggctc ggcccttgta
    13321 ggagcgggct tgcccgcgaa tcgcggccga atagaacaac atcgcggcca aggccgctcc
    13381 tacgggtttc gcgacaaccg ccagcacagc tggaaacact tgactggtac atagccatta
    13441 tcaaaggcga aacctgcacc aaccgtccgc cgcgcatgct gcaggcctgt tccagcagca
    13501 ggatctacag cgcaaacgcc cgcaccgcga cgctcatgcc acctcatagg ttcttgttat
    13561 gctccccgcc taacccatca acccgcagca ccgaaacgcc atggacagta tcgacaccct
    13621 gattatcggc gcaggcgcca tcggcctggc ttgcgccgcg cgcctggcca ggccgagcca
    13681 gagcaccctg atagccgagg ccgagggcct gatcggcagc cacacctcca gccgcaactc
    13741 cgaggtgatc cacgctggca tctactacgc gcccggctcg ctcaaggccg aggtgtgcct
    13801 ggaaggccgc gaacgcctgt atgcctggtg ccacgcgcat caggtcgcac actgccagct
    13861 cggcaagctg ttggtagcgg tgaaaagcgc ggaaatcgcc aaactcgaac aactgcaaag
    13921 caatgctcag gcctgcggcg tacacgactt gcagcccatc gagcagaaac gcctgcacgc
    13981 gcttgagcca gccgtgcgtg gggttgccgc cctgttctcg cccagcaccg ggattatcga
    14041 cagccacgcc tacctgcaat cgctgctggc ggccgcagag aatcgcggcg cacagctggt
    14101 tctgcacacc cgcgtcgaac aactcgaagc aagcagcgac ggctggatag cctcgggcca
    14161 cagcagcggc gagccgttcc agctgaaagc ccaacaggtg gtcaatgccg gcggtctgtt
    14221 cgcccagcaa ctggccaacc aaacccaagg gctgcaaaac gtgccgccac tgcacctatg
    14281 ccagggccgc tacttcagct acagcggccg ttcgcccttc cagcacctga tctacccgat
    14341 gccggaagcc aataccaccg gccttggcat ccacgccacc ctggatctcg gcggtcagct
    14401 gcgtttcggc ccggataccc gctacctcac gcagatcgac tatcaggtcg atgaaaccct
    14461 gcgcgaaccc ttcgccgaag ccatccgccg ctacttcccc actatcgaca gcgcgcgcct
    14521 ggtgccgggc tatagcggta tccggccgaa actcgcaggc cctggggaac cggccgccga
    14581 cttcattatc caaacgccca acgagcatgg cctgccaggc ctggttaacc tgttaggcat
    14641 cgagtcgccc gggcttaccg ccagcctggc gattgccgag cgcgtggcgc aatcactgta
    14701 aaagccgctt gcaaaatcac tgcgtaaacg ggactgtcgg cggtcaaggt aaaaccgctg
    14761 gcgctatact cgccggcttg aattgctgct ctacaccttc cagtttcgaa acaaggattc
    14821 gcacatgaaa gcgctcggca aaatcctcgg cctgtttttt ctcgggctat tgctgatcat
    14881 cgtggcactg ggctttgccc tcacccacct gttcgatccg aacgattaca aagacgaaat
    14941 ccgccagctt gcgcgtgaca aagccaatct tgagctgacc ctcaagggcg atatcggctg
    15001 gagcctgttc ccctggcttg gcctggagct gaccgacgcc acgctcgcca gcctaaacac
    15061 cccggacaaa cccttcgccg acctgcgcct gctcggtttg tcagtgcgcg tgctgccgct
    15121 gctgcgtaaa gaagtgcaga tgagcgatat ccgcatcgat ggcctcaacc tcaacctgca
    15181 actcgacgaa aaaggccgca gcaactggga agaggtcggc aaaccggcca aagtcgcgac
    15241 cacagccacc gacgcgtcag tagcaagcag cgatagcact ccggccaaca gcacagccgc
    15301 agagcctgag agcagccagc cactgaaact ggatatcgac agcctgatcg tcaacagcgc
    15361 gcgcatcgac taccacgacg cgcagaaagg ccagcaattc agcgccgaaa gcatccagct
    15421 gaccaccggg gcgatccgcg aaggcgccgg tatcccgatc aaactcagcg ccttcttcgg
    15481 tctcaacaag ccggtattac gcgcccgtag cgagctgcag ggtgaactgc gcttcgaccg
    15541 cgcactgaaa cgctaccaat tggaagacgc caaactctcc ggcgaagtgt ccggcgaacc
    15601 ctttgacggc cagaccctga cctattcggc ccaaggccaa ctgctggtcg acctggccgc
    15661 ccaggtcgcc gagtggaacg gactgaaatt ctccggcaac cagctacgcg ccctcggtga
    15721 gctgaaagtc cgcgacctcg acaaacagcc aaaactcagc ggcggcctgt ccatcgcacc
    15781 gctcaacctg cgtgaattcc tcgccaccat cggccagaaa ctgccgccga tgaccgatga
    15841 cacgaccctc agccagttcg aactggtcac ccgcctcagc ggcaccgaca acagcctgac
    15901 cctcgaagaa ctcaaactga ccctggatga cagccacttc agcggccaac tggcgattgc
    15961 cgatttcgcc aagcaaaccc tgcgcgtgca actcaagggc gaccaactca acctcgaccg
    16021 ttacctaccc gcaccctcca aggacgccaa ggatgccggc gccgcacgca aagccgaggt
    16081 caaggccagc gttgccagtg ccgggcagag cggcagcaca ccgctgcccg atgcaccgac
    16141 tcagcacgcc tggagcagca gcgaagtgct gccgatcgag acgttgcgca agctcgacat
    16201 gcaactcgac ctcggcctcg atcagctcac cgcccaacag caggtgatca gcaacttcag
    16261 cgccaaggcg caaagcaaag gcggcctgct gaccctgcag gatgtgcgcg gcaatatcgg
    16321 caacggcagc ttcgacgcca aggccagcat cgacgtgcgc ccagccatac cgctgctcag
    16381 cgtgcagaaa aacctcagca acatcccggt tgaaccattc ctgaaaaagc ccgaccagcc
    16441 ttcgccggtc aaaggcctgc tggatttcaa agccaacctg accaccagcg gcaacagcca
    16501 gcgcgcctgg gtcgatgggc tgactggcga tgctagcttc ctgctcaacg acggcgtgct
    16561 ggtcgacgcc aacctggaac agcagctgtg ccgcggtatc gccaccctca accgcaaagc
    16621 cctgagcagc gacccacgcg gcaaggacac gcccttcgaa actctgcaag gcagcctgat
    16681 cctgcgcaac ggcgtggccc acaaccccga cctcaaggcc gttattccgg gcctggcggt
    16741 cagcggcaat ggtgatctgg acctgcgcat cctcggcctc gactaccgcg tcggcatcac
    16801 catcaccggt gatcaacgcg aaatgcccga cccggcctgc caggtcaacg agcgctatgt
    16861 tggcatcgaa tggccagtgc gctgccgcgg cccgctggaa atgggcggca aggcctgccg
    16921 cctggatcag gacggcctgg gcaaagtcgc cgccaaactg gccggcaaca agctcaacga
    16981 gaagatcgaa gaaaaactcg gcgacaaggt cagccccgag ctgaaagacg cactcaaggg
    17041 cctgttcaac cgatgagccc cgagcagttt tctgacgcag tactgaactg gtacgacagc
    17101 cacggacgca aggatctgcc ctggcaacag ggcatcacgc cataccgcgt gtgggtctcg
    17161 gagatcatgc tgcagcagac ccaggtcagc accgtgctcg gctatttcga ccgctttatg
    17221 gccgccttgc cgacggtaaa tgacctggcc gcagccccgg aagacgaagt gctgcacctg
    17281 tggaccggcc tcggctacta cacccgcgcg cgcaacctgc aaaagaccgc gcagatcgtt
    17341 atgcgcgaat atgctggcga attcccccgc gatgtcgacc agctcaccga acttcccggc
    17401 atcggccgct ccaccgccgg cgccatcgcc agcctcagca tgggcctgcg tgcgccgatc
    17461 ctcgacggaa acgtcaaacg cgtactggcg cgctacgtgg cccaggaagg ctatccgggc
    17521 gaaccaaagg tggccaaaca gctgtggggt gtggccgagc gctttacccc gcacgcccgg
    17581 gtaaaccact acacccaggc gatgatggat ctgggcgcca ccctgtgtac ccgcagtaaa
    17641 ccgagctgcc tgctctgccc gctgaaaagc ggctgccagg cgcatctgct cggcctggag
    17701 atccgctacc cgattcccaa accgcgcaag gcactgccac agaagcgcac gctgatgccg
    17761 attttcgcca accgccacgg cgaggtgctg ctctatcgcc gcccgtccag cggcctctgg
    17821 ggcggtttgt ggagcctgcc agagctggac gaagcaacgg ccatcgcctc actcgccagc
    17881 cgccacaccc tgcagctcgg cgcacagcgc gaactgagcg gcctgaccca caccttcagc
    17941 cacttccagt tggccatcga accctggctg atccaggtcg acgccgcgcc agccgccgtg
    18001 gccgaggctg actggctctg gtataacctc gccaccccgc cgcgcctggg cctcgccgcc
    18061 ccggtgaaga agttactgaa gcgcgcggcc gatgcactcg cagcaaatac tggagaaacg
    18121 ccatgacccg caccgtacag tgccgcaaac acaagcaaga actccccggc ctggagcgcc
    18181 caccgtatcc aggcgccaaa ggtgaggaca tcttcaacaa cgtctccaag caagcctggg
    18241 acgaatggca gaagcaccag accctgctga tcaacgaacg ccgcctgaac atgatgaacg
    18301 ccgaagaccg caaattcctc caggtcgaga tggacaaatt cctctccggc gaggaatacg
    18361 cccaggccga cggttacgtg ccacctagcg agtaagcggc caggcactac acccgtagcc
    18421 cggataaagc gcggcacgcg ctgcaatccg ggacggcacc ctcccggatt gcgccaaggc
    18481 ttatccgggc tacccaacca ccctgtacac ccgcccccga accctcgccc gaacctaagc
    18541 gcccgtaata attaaatatt tttcaaactc acgcttgaca cctacccctc aaatccgtct
    18601 aatagcgccc cgttagcccg ggtagctcag tcggtagagc aggggattga aaatccccgt
    18661 gtcggcggtt cgattccgtc cccgggcacc aaatacagag aaaggctcac agcgatgtgg
    18721 gccttttctt tttctacaga aagctatctg cagaaaaagc tggcgacctc gctcgccctg
    18781 cgccatgttc agcaccgtca gccaccaggg acgcacgctg ccatgcatga cccgattctc
    18841 tacagcaccg acgacggcaa gcttacgctg caactgcgtg cctgcgtctg ggcgaaccag
    18901 caggcattcg ccaagcggct tatcagcctg cctatccgca gcacgcccta ccgaggcgca
    18961 cccagcccgg caactatcaa gcaatccttg acagttcagc ccgtggccag ccgccagatc
    19021 agccgcagcc ctcagcgcga tgcggcactc gccgtccgcc aaccctgatt cagccaagaa
    19081 gaagcccatg agccatagcc ccatcagcca ggacgaaatc aacgccatca tctggaagtc
    19141 gtgcgacacc ttccggggca gcgtggatgc cagcgactac aagaactacg tgctaaccat
    19201 gttgttcttg aagtacctga gcgacgtctg gcaggaccac tacgaaggct atgtggccaa
    19261 atacggcaat gagccggcgc tgatcgccgc catgatgaag accgagcgct ttgtgctgcc
    19321 cgcgtcggcc aacttctaca gcctgcacga caaacgccac ctgcccggca acggcgagcg
    19381 tatcgacgaa gctctgcatg ccatcgaaga cgccaatagc gccaaactgc gcggcgtgtt
    19441 ccaggacatc agctttaacg ccaacaaact cggcgaagaa aaaagcaaaa acaacctgct
    19501 gcgccacctg ctggaagact tcgcccaggc ggacatgaac ctgcgcccct cgcgcatcgg
    19561 cagccaggac attatcggca acgcctacga gtttctgatt tcgcgctttg ccgccggtgc
    19621 cggcaaaacc gctggcgagt tctatacccc ggcccaggtc agcgacctgc tggccgaact
    19681 gctcgatccg cagcccggcg agcagatttg cgacccagcc tgcggctcgg cctcgctgct
    19741 gatgaaatgc gggcgcaagg tgctggagaa aaccggctca cgcaactacg ccctgtttgg
    19801 ccaagaatcc atcggcgcca cctgggctct ggccaagatg aacctgtttc ttcacggcga
    19861 ggaaaatcac gacctacgct ggggcgacag catccgccac ccgcaactgc tggatgccaa
    19921 cggcggcctg atgcacttcg acgtggtcac cgccaacccg cccttctccc tggagaaatg
    19981 gggcgtcgaa caggctgaga acgacaaata cggccgcttc cagcgcggca tcccgccgaa
    20041 aaccaagggc gactatgcct ttatcctgca tatgatcgaa accctgaaac cgggcaccgg
    20101 ccggatgggc gtggtagtac cccacggcgt gctgttccgt ggcggcagcg aaggcaagat
    20161 ccgccaggcg ctgatcgagg aaaacctgct ggatgcggtg atcggcctgc cggaaaagct
    20221 gttcttcggc accggcatcc cggctgcgat tttgctgttc cgcaaaacca agagcacgca
    20281 agacgtgatc tttatcgacg cctcccgcga ctttgtcgcc ggcaaaaacc agaaccagct
    20341 cggcccgcag cagcttgggc gtatcgtcag cgcctaccgc gcccgccagc accagagccg
    20401 ctacgccgaa tgcgtcagcc tgcaagccat ccgcgataac gactacaacc tgaatatccc
    20461 gcgctatgtg gataccttcg aggctgagga cgagattgat ttgttggcga tcaaggctga
    20521 gcgtgagggg ttggagcggg aactggtggc gttaaagcgg gagatggatg ggtatttgga
    20581 ggagctgggt tatggtgcct aagggctggg aggtcaaaag ccttggcgat atagccacga
    20641 tttcttctgg aggcactccc agccgctctc agagcgaata ctgggatgga gacatcccct
    20701 gggttacgac aacagaagtc aacttcggaa ccattaccaa tacagcccaa aagatcactc
    20761 aactgggcat gcagtgctca tcggcaaagc tattcccgaa gaacaccatt ctgatggcca
    20821 tgtacggcca agggaaaacg cgcggtcagg ttgccaagct agggatagag gcaaccacaa
    20881 accaggcctg cgccgcgata atccccaaaa gtggtcactc acacgagttc ctttttcagt
    20941 acctgcaggc cgaatatgaa aatattcgca gcatgagtaa tggcggtggt cagaacaact
    21001 taagcgctgg cctgattaaa gaaatcccgg ttcccgtacc gccgcttgca gaacaaacca
    21061 agatcgccca aatcctttcc acctgggata aagccatcac cactaccgag cgcctgcttg
    21121 ccaatagcca gcagcagaag caggcactga cctggcaatt gctgactgga aaaaaacgct
    21181 ttgcggattt cagtgacact tggggcaagc agaaactaag cgaaatcagt acccgcatcc
    21241 aagaaaggac cgacggggaa gcgcatccca tcctgaccat ttcgtcactt tccggctttg
    21301 tcacccaaga ggagagatac agccgctaca tggccggcga aagcgtcaac aactacatac
    21361 tgctgagccg aggcgagttc gcctacaaca aaggcaactc caaaacctac caatacggct
    21421 gcatcttcaa actggatacc ttcgacaccg gcctggtgcc ccacgtctac gtgtgcttca
    21481 aacttaatca ggggttcgac ccggagtatt acgctcaact gtttgccgcc gactacctga
    21541 aagatcaact agccggcctg gttaacacag gcgtacgcaa taacgggcta ctcaatatca
    21601 agcccagcgc attcctcgac accacggtgc ccgttccacc actggccgaa caacaaaaaa
    21661 tcgcccaggt actctccacc gccgacgccg aaatcagcaa tctgcaagcg caactggcca
    21721 agctaaagct ggagaaaaaa gccctgatgc agcaactgct taccggccag cgccgggtgc
    21781 ggctggacga cgaggtggaa gaagagccac agatccgtag ggtgggttag gccgcgcccg
    21841 ctcccggcgg gcttgcggca ttcaccccat ccatggggat tgcgcctgtc acgggcgcaa
    21901 aggccacggc caagcccgac tgcgccgtaa cccaccatgg cggtctaccg gtaaaccgca
    21961 atgctgggtt acgcagcgca cgacctgcgt ggtgcggcca acaactacga cccgcgccgc
    22021 taatccaccc tacgatctgg ccggcgcagc gccagactcg gcgatagctg acggcctctg
    22081 gtatcgtctg ccaatcgccc cgcaaggaag ccccttgcag cccgaagtga tcaaccctct
    22141 gcaacaggcc tgtgtgaccc tcgctgccgg tcacctcatg gttggccgaa caaggaatag
    22201 tgctgtgacc accacgcccg cctttgccca ccccaagttt caagaagagt tcagcgccaa
    22261 gatccccgcc ttggcgttgt tggccaatct gggttttacc tacctaagcc caagccaggc
    22321 gctggcactg cgcggcagca aggagcgggt ggtgttgccc ccggtgctac gcgccgaact
    22381 ggccaagcgg cgctttttca gcaatggcgt agagcatggc ctgagcgagg cggcgctgaa
    22441 caagctggtc agcgagatca gccatccacc gctgaacgag ggtttacagg cggccaacca
    22501 gcgtctgtcc gaccagttgc tgtacggggt gagcgttggc gattttgtcg ccggggccaa
    22561 ggttaacccg accatcgcgc tgatcgactg gggcgacctg agcaataaca gctttcacct
    22621 gaccgaggag ctgggcgtgg gttgcaccag cggtatcggc cagcgcatcc cggatatcgt
    22681 gctgtatgtg aacggcctgc cactagtggt aatcgaggcc aagcgtccgg atgccaataa
    22741 cggcaaagac ctgattcagc agggtatctc gcagatgctg cgcaaccagc gcgccgatga
    22801 aatcccccag ctatttgcct ttagccagct gctgctggtg gtcgatggca gtggcgggcg
    22861 ctacggcacc gtgggcacca ccgagaagtt ctgggccgcc tggcgcgaag aggaaatcgg
    22921 cgaggtggcc ttcgccgagc tgaagaaccg ccctctggac gctacccagg agcaggcgat
    22981 attcgcccag cgccccgccg cgagccttgc ctggtatcgc agctggctgg ccggcgggtt
    23041 gctcgccagc cagcaggaca aactgttggc cagcctgtgc cggccggatc gcctgctgga
    23101 gctggtgcgc ttttacacct acagcgatag caagcacggc aagatcgtcg cgcgttacca
    23161 acaagtgttt ggcatccgcg cgctgatcga gcgcatccag cagcgtgatg tgcgcggcgc
    23221 acgccagggc ggggtggtct ggcataccac cggctcgggc aagtcgatca ccatgctgtt
    23281 gctggccaag gcgctgatct tccacccgca gctgcagaac tgccgggtac tggtgatcac
    23341 cgaccgcacc gatctggaag cgcaactgag caagaccttc gccagcgccg gcgtgctcac
    23401 cgagcgcgat cagcaggaag ctctgagcca atcaggccgg cacctggccc agcagatcgg
    23461 caaaggctcg gtgcgggtga tgttcagctt gatccagaaa ttcaattcgg cggccaagct
    23521 gccggaatgc cacaacgaca gcagcgactt tatcgtgctg gtagacgaag gccatcgctc
    23581 ccagggcggc gaaaaccatg tgcagatgcg cctggccctg cccaacgcgg cctttatcgc
    23641 ctttaccggc acaccgctgc tgctgaagga aaaaacccag agcaagttcg gccccatcgt
    23701 gcacgcctac accatgcagc gtgccgtgga agacggcacg gtgaccccgc tgctgtacga
    23761 agagcggcgc ctggaactgg atgtaaacga ccgcgccatc gacagctggt tcgaacgcag
    23821 cacccgaggc ctgagcgagg cgcagcaggc cgacctgaaa aagaagttcg ccaagaaggg
    23881 tgagctgtac aaggccctgg gccggctgga gctgatcgcc catgacatca gcgagcattt
    23941 cagcagctcg gtgctggacg gtctgaaagg ccaggtggcg tgtgacagcc gggtatcggc
    24001 gattcgcctg aaggaatttc tcgacgagat cggccaggtc agctcggcgc tggtgatgtc
    24061 cgccccggat agccgtgagg gcaatagcga cagcgacgag gacagcaagt cggtggtgca
    24121 ggcctggtgg gacaagcatg tcggcaagga agacggcacc gtctacaagc aacggatgat
    24181 cgacgccttt gctcaggacg atggccccga cctgctgatt gtcatcgacc agttactcac
    24241 cggcttcgac gaaccgcgca atgcggtgct gtacatcgac aagaacctca agcagcacaa
    24301 cctgatccag gccatcgcgc gggtcaatcg tctgcacccg ctgaaaaagc acggcctgct
    24361 gatcgactat cgcggcatcc tcggcgagct ggatatcacc atcgcccaat atcaggacct
    24421 ggccacgcgc acccagggcg gctacagcat cgaggatctg gatggcctgt acagcccgat
    24481 gagcaccgaa tacaagaagc tgccgagcct gtaccagcag ctgtgggcga tgtttaagga
    24541 cgtgaagaac acccaagaca tcgagcagat gctggcggtg ctgctgccgc gcctgcagga
    24601 ggtggatggc gcgcaggtgg atacgcggca gaaggcccgt gatgacttct atgcgttgct
    24661 cagcgagttt gccgcctgcc tggcaattgc cttgcagtcc caggcgttct ttcaggacaa
    24721 aagcgtcagc gaggacaccc gccgccagta caaggaaacg gtcaagcaac tgtccaatct
    24781 gcgccagcgc ctcaagcagg caaccggcga taccgtggat tacgacgagt acgcggtgca
    24841 ggtgaaaaag ttgatggaca agcatgtgct tggcatcggc gtgcgcgaat ccaagggcgc
    24901 ttacgtggta ggcgatatgg gcgcacacaa gccggaggcc tggagcgagg agaagacccg
    24961 caacgagaag gacatcatcc agacccggct gaccaaggaa atcgaccagt tgctggacga
    25021 tccttatgcg cagaagcgtt tctccgagct gctacgccag gtcatcgccg aggccgatgc
    25081 gctgttcgat aacccactca agcagtacct gctgttcaag tcgttcgacg agcagctgca
    25141 gcagcgccag ctcgacgagc tgcccgatgc ggtacgcggc aacccccatg tgcaggcgta
    25201 tttcggcgct ttcaagctgc tgctggatga gcagcgtgtg gccgccgagg ccatcgacac
    25261 gccgcactgg gttctacttg ccgagcagac cgacgccatc gtcagccagg cggtacgcga
    25321 gttttccatt aacccgttga atatcgaggc agccgtgcgc cagcaactgc tgccgctgta
    25381 ctttatggcg ctaaaagccc tgggcctggg tatggatcag gtgcaggcgc tggtggaaca
    25441 ggtggtgcag atcgtccgcg ccggagcgcg caaagcctga tggccagcct gcattacggc
    25501 ggcgagacga ttgcctatca ggtgcagcac gttccacgcc cggcgcgcaa actgaccatc
    25561 aaggtgcagc ccgatggcca ggtgctggta atggcaccgg ccgatgccag cgccgagcag
    25621 atcgctgccg gggtcaacaa acgcgcacgc tggatctggg agcaactgca gccgatccgc
    25681 gaacgcctgc tgcatgtgcg cccacgccag tacatcagcg gcgaaaccca tttctatctg
    25741 ggtaagcgct acccgctgaa agtccaggaa gacgcccgcg caccgcaaca ggtcaagctg
    25801 ctgcgcggca tgctgtgcat cacggtgcgg cagaatcgcc cggagaaagt tcgcgagttg
    25861 ttattcgcct ggtacaaagc ccgcgcccgc gagcagttca atcagcgtct agagcgcctg
    25921 acgccacagg cccgctggct aacgcaaaag ccggcactgc gcctgctgac catgcagacg
    25981 cagtggggta gttgctcgca ttccggcacc ctgacgctca acccacacct ggtcaaagct
    26041 ccgcccgaat gcattgacta cgtgttgctg cacgagcttt gccacctagc cgaacacaac
    26101 cactccgagg ctttttggcg cttgctcgat caattgatgc cggactggca gccgatcaag
    26161 gcgcgtttgg atgagatggt ggagttgtat ttgaatgatg tgcgttgagg cgacttttta
    26221 tggcctgcca tccatggcgg ccacctttcg ggccgtcgct gcgcgacgtt taaaatgttt
    26281 cccgtccatt tttttgcatc tgcgcgacaa ccaacagcaa ccaggcgaca ccgtgaccgg
    26341 ccaagcccgg cgcggccggt aaacggcttg gcggtcagcg aaacgctatg ctagcttgac
    26401 gcctcgccag ggaggccacg caacgcctga gcgcaaccga agaataagag gacactccat
    26461 ggccgaggcc acgcccgcgc tggaaatacg cgacctacat aaacgctacg gcgaccttga
    26521 ggtgctcaag ggcatctccc tcaccgctcg cgacggcgat gtgatttcca tcctcggttc
    26581 ttccggctcc ggcaagtcga cctttctgcg ctgcatcaat ttgctggaaa acccgcacaa
    26641 ggggcagatc atcctcgcgg gcgaagagct caagctcaaa gccgcgaaga atggcgacct
    26701 ggtcgccgcc gacagccgcc agatcaatcg cctgcgcagc gagatcggct tcgtcttcca
    26761 gaatttcaat ctgtggccgc acatgagcgt gctcgacaac atcatcgagg cgccacgccg
    26821 cgtgttgggg cagagcaaga ccgaggccat tgaggtggct gaggcgctgc tggccaaggt
    26881 cggtattgcc gacaagcgcc acgtttatcc gaatcagctt tccggcggcc agcaacagcg
    26941 cgcggcgatt gcccgcacgc tggccatgca gccgaaagtc atcctgttcg atgagcccac
    27001 ttcggctctc gacccggaga tggtacaaga agtgcttaat gtgatccgcg cactcgccga
    27061 agaaggccgc accatgctgc tggtaacaca tgaaatgggc tttgcccgcc aagtgtccag
    27121 cgaggtggtg tttctgcatc agggcctggt tgaagaacag ggctccccgg agcaggtgtt
    27181 cgacaacccg aactcggcgc gctgcaaaca attcatgtcc agcaatcact aaaaacacgg
    27241 agcaactacg catgcagaac tataagaaga tcctgctggc cgcagccgcc accctggcct
    27301 tcggcaccag cgccgtcgcc gcggacaagc tgaagctcgg caccgaaggc gcttatccgc
    27361 cgttcaacct gattgacgcc agcggccagg ttggcggttt tgacgtggaa atcggccagg
    27421 ccctgtgcgc caagatgaag gccgagtgcg aagtggtcac ctccgactgg gacggcatca
    27481 tcccagccct gaacgcgaag aaattcgact tcctgatcgc ctccatgtcg atcaccgaag
    27541 agcgcaaggc cgcggttgat ttcaccgagc cctactacac caacaagctg cagttcatcg
    27601 ccccgaaagg cagcgacttc aagactgata aagccagcct caaaggcaaa gtgatcggcg
    27661 cccagcgcgc gaccatcgct ggcacctggc tggaagacaa cctggacggt gtggttgata
    27721 tcaagctgta cgacacccag gaaaacgcct acctcgacct ggcttcgggc cgcgtcgacg
    27781 gcgtgttggc tgacacgttc gttaactggg aatggctgaa gagcgacgct ggcaagagct
    27841 tcgaattcaa aggcgacccg gtattcgaca acgacaagat cggcattgcc gtgcgtaagg
    27901 gcgacgccct gcgcgagaag ctgaacgccg cactggccga gatcgtggct gacggcacct
    27961 acaagaagat caacgacaag tacttcccgt tcagcatcta ctaagctgcc ggcactgcgc
    28021 agcccgtccg ggcggcgcag gcttctgagc ccctatgaat ttcgacctct acggattcgg
    28081 cccggccctg gctgctggca ccctgatgac catcaagctg gcgcttagtg cgctgtgcct
    28141 gggtctggtg ctcggtttgc tcggcgccct tgccaagact tccccgtaca agccgctgca
    28201 atggcttggc tgcacttact cgaccatcgt gcgcgggatt cccgagctgc tctgggtgtt
    28261 gctgatctat ttcggcaccg tgcagctgat gcgcaacctg gcagacctgc tcggcatcga
    28321 aagccttgag ctgaacgcct tcgccgccgg gaccatcgcc ctcggcatct gcttcggcgc
    28381 atacgccact gaggtgtttc gcggtgcgat cctggccatc cccaagggcc atcgcgaagc
    28441 gggcctagcc ttgggcatgt ccaagccacg gatcttctgg cggctgatcc tgccgcagat
    28501 gtggcgcatc gccctgcccg gcctgggcaa cctgtttatg atcctgatga aggacaccgc
    28561 gctggtgtcg gtgatcggcc tggaagaaat catgcgccgc tcgcaaatcg ccgtaacgtc
    28621 cagcaaagag cctttcacct tttatatggt ggcggccttt atctacctgg gcctgaccgt
    28681 cgtcgcgatg atcggtattc acttccttga aaaacgctcc agccgtggct ttgtcaggag
    28741 cgcgtcatga gaaagttttc tgaaatagcc gcctggtcgg ttgccgttgc gctgattgcc
    28801 tatgcactct gggggggcct gaccgccctc aactgggacg tgatcatcaa gtggctgccg
    28861 cgcttgtctg aaggtgccct gctgaccctg gagctggtgg ccatcgcggt tcttgccggg
    28921 ctgattctcg ccctgcccat gggcatcgcc cgcgcgtcgc ggcactggta tgtgcgcgcc
    28981 ctgccctatg gctacatctt cttcttccgt ggcacgccgc tgctggtgca gctgttcctg
    29041 gtctattacg ggctggcaca gttcgatgcg gtgcgcgaag gtccgctctg gccttatctg
    29101 cgctccccct actggtgcgc ggtgctgacc atgaccatgc acaccgccgc ctatattgct
    29161 gagattctgc gtggtgcgat tcaagccgta ccaccaggcg agatcgaggc tgctcgcgcg
    29221 ctgggcatgt cgcgagccaa gaccatgttc tatatcgtcc tgccacgggc ggcgcgcatc
    29281 ggtctgccgg cctacagcaa cgaagtgatc ctgatgctca aggccagcgc cctggccagc
    29341 accgtgactc tgctggagct gaccggcatg gcccgcacca tcatcgctcg cacctaccta
    29401 ccggtggaga tcttctttgc agctgggatg ttctacctgc taatcgcctt cctgctggtg
    29461 cgcggcttca agctgctgga acgctggcta cgcgtcgacg cctgccaggg ccgctgattg
    29521 cctgagggct cgcgccctca ggcaccatcc atcatgccaa tcaccacccc aacccaggtc
    29581 ctgcaagacg ccgccctatt gcagcgtttt caggcgctcg acagctttct tattgagcac
    29641 caagcgctgt ggcgaccacg gccctttgtg cattacccgg cattgccctg ggaagccgag
    29701 cacccacagc tggcagactg gctcagggca caaagcctgg agcatgcaga agccgcacat
    29761 aaccagccgc acctgctgca agcacccgcg cccttcccgc aattggccgc tcaggctcac
    29821 tacttaagcc ggacaggtga actgccgcag tcacccgcgc aggtattacc cgcgcgcttt
    29881 agcgcagatg tgccgggtcg caaatggcag cagatagacg cttttgcccg cagcctgagc
    29941 tttatccaga aaccgcaaca ctggctggac tggtgcgccg gtaaaggcca ccttggccgg
    30001 atgcttgcac acggcggcgg caacctgact tgcctggaat acgacccgct gctggtcagc
    30061 aacggccaac aactcagcaa taaactcggc atcaccgcgc agcatatcga gcaggatgtg
    30121 ctggccgacg acgcggcgac gcagctgcaa gtgcaacaca cgcccgtggc cctgcatgcc
    30181 tgcggcgaac tgcacacacg cctgctgcaa ctggccagcc aggccggctg ccaacaactg
    30241 gccgtggcgc cctgctgcta caaccgcatc agcacacagc gctatcagcc gctgtcggca
    30301 agcgcccaag cctcgacact gcaactgtcc atcgacgatc tcggcctacc cctgagcgaa
    30361 accgttaccg ctggcgcgcg ggtacgccgc cagcgcgatc aatccatggc cagacgcctg
    30421 gccttcgact tgctgcaacg ccagctgcgc ggcatcgatg aatacctgcc caccccatcc
    30481 ctgccagcgg cctggctcga caagcccttt gccgattact gcaaggacct cgccgcactc
    30541 aagcaactgc ccacacccgc gccccaggat tggcaacagc ttgaacaaaa tggctggcaa
    30601 cgcctggccg aggtgcgcaa tctggagctg ttgcgcggct tattccggcg cccactggaa
    30661 ctctggctac tgctcgaccg cgcgctattc ctgcaggagc aaggctacga cgtgcgcctg
    30721 ggcaccttct gcagccacca acagagcccg agaaatctgc tgctactggc agagcgccag
    30781 taaaaattac ccacatagac tgtggataac tctgttgaca gaatctgagc aaaccacgca
    30841 aaagcccgcc ccagctgcat atcgtcaacc tgcacatttt ttaatctgaa aatatctctt
    30901 gcaaaaacat gcacttagcc aataccgcga accagcgcac aaagtctgcg cacgctcgtg
    30961 ccagatgccc tgcatatgtg cataagcttt ttgctgctga taacaatgcc tgcacagaac
    31021 tacgctcaga cggctttact cagccaaacg aatggatata atggcgaccc tcaaaggccg
    31081 gtatagctca gctggtagag caactgactt gtaatcagta ggtcccgggt tcgattcctg
    31141 gtgccggcac catacgaagc aacgacttag gctcaccgca aggtgggcct ttttcgtata
    31201 tgggatttgg ggtcatcgtt tctcttgcac tgatctgcac accgtcgagc tttgatcgca
    31261 ttcaccctgg tgtcacagcg agcatcgccg tgcgcccctt cgacctgacc gctactccag
    31321 caacgctgaa accagctgcg acgtttccgc tatccatccg ttcacggcat gctccctcga
    31381 gcactgaatg acttggctga taaccaccac cgggatgatg tcggtgtagt tcgtacggtc
    31441 cgcgccaagc accttgcctc agccgtctgc agcagataac gaacgccggt cttatggttg
    31501 ctgagctgta cttttcggga aaggactttt tcagaccatc cctaagcttt caacaaccta
    31561 cccaaagtcg gacaggcgta ctactcggtc agctcgacga gctcttgccc agaattgata
    31621 cccctacttc gcctacaaaa gacctgttag cggccctcac gactgtagtg cgccccccga
    31681 tcgtagacct ccacgacctt gcactcgact tttgagctga actgtaattt gttgtcgtcc
    31741 gcatagggca cataaaaaag aattgcatta tcactatacg caggttcaag acactgataa
    31801 gtacgaatat aaagaagatc atccatagta gagaacacat ctcgatcata ttgtttaata
    31861 acgaccctca ccggatcgtc gtcagcagca taaatagaag atgaaacaac aatacagaac
    31921 agaaaaaaca atatagtaaa acaccttaaa tataattctc taatcatcct taaaccccac
    31981 ctgtgcctac cgtttatttt caccaaagcc accctgtaac gcctcaactt aggcgaatga
    32041 aaagccaccc cgcgccagga accagtagct acagaccttg ccagctaatc ctgcataatg
    32101 gcatgctgcc ataagcgaag cacatgaaat cccttatagg gtaaacactc aaaaacttac
    32161 attcgccgcc aaattattga tgtcattttt ccgccgacac taagcccaca ttagcattaa
    32221 agacacacat ctaggagcat gacatgtcga agctggatac tcttcaggcg tttcgttgta
    32281 ttggcctgac aggcgtggtc atcggccaca tgacattcgc cgaggaaaag tattctggcg
    32341 ccactttatt accgctcgca ataaaagact tcaaccagct gttcatcgac tggttcttcg
    32401 tcgtcagcgg cttcatcatg gtcatggttt cacgcgggcg cttccaggta ccgcgggaga
    32461 tactgaagtt cgtttatgcg cgactggccc gcatctatcc gccgtactgg gtgtacttct
    32521 tcattaccct tgcggtgttc ctggccatgc ccgagtgggt taacagcgga tataccgagc
    32581 ccaacctgtg gaagtccttc ctgctgatac cggacaaaac cctacctctg gtaatggtcg
    32641 cctggtcgct gatcttcgag atgtggttct atctggtatt cacctccttc atgctttgcc
    32701 gcgaacgcca tttgccttgg attttggcag gctgggcctg cctgattatc tgcgccaacc
    32761 tcatactcga actgtcgagc ctcagcccgg ctctgcgtat tgccttgcat ccctttgcga
    32821 tcgagttcgt catgggcggc ttcatcgcgc tgttctactt cagccctctg agtgaacacg
    32881 tctccaaccg gatggctgtc gcaatgctgc tattcgtgcc gctgatcggt gtgcccgtcg
    32941 gttatagttt tggactcttc cgaggggacg gcctgatccg agtgttcagc ctgggttgcc
    33001 tatttggcat gctgactatc ggcgcaacac tgcttgagcg ccgcgaaaag ctgagtgtac
    33061 ccagcctaat ggtggctatc ggtgacagct cctacaccac ctacctggct catcttctgg
    33121 tgatcggtgc ggttggcaaa ctgtgggtct tactggggct gtcagcgtca aacctgatca
    33181 ataacctggc ctttctggtg atcatgttcc tggccgttat gatctatggg ctggtcgcct
    33241 ttcgccttat cgaggagccc ctgatgaaca gcgccaatca tctgggcaaa cggctattcc
    33301 gcaagaaaca gcaagacact gccacactga ctgcgaaaac cattaagtga acagcgccac
    33361 catgacgcgc cacagtccac gcttgcggca gaacaacttc gacctgatcc gcctgctgtt
    33421 agccttggcg gtttgtctgc tgcatgccca tgaactgtca ggcgtggcag atctgagcat
    33481 catagacacg ttgctgtcag ggaagattgc cattgagtca ttcttcgtca tcagtggctt
    33541 cctgattttc atgagttacg aaaagtcacg ctcactggct gactaccttg agaaacgtgc
    33601 ccgtcgcatc tacccggcct acttcgcttc cgtgctgttg gccgcgttac tgctttccag
    33661 catcagtgag cgcccactgg ctgcgtactt tttctcgagc gattttattc gctacctggc
    33721 ctccaactta ggctttctca actttatcca gcccgatcta cccggagtct tcaccgagaa
    33781 ccgcctcgca gccgtgaacg gggcactctg gaccatcaag atcgaagtga tgttctacct
    33841 gagcgttccc atcatcgtct ggttaagcag acgtgtcggc cgcgccccga cgctcctcgc
    33901 gctgtttgtc gcctcgactg ccttctccct gtacatgctg cacctgcatg aaatcaccgg
    33961 caggaatcta tatgtcttac tggagaagca actgcctggg cagttgcgtt tcttcattgc
    34021 tggtgcggcg ctctattact tgctccccta cttcgagcgg cacataaaac tcctagtgct
    34081 ggctgcagtg ctctacttcc tggcgcagtc atgggttgaa ctcgaagccc tcaaacctgt
    34141 tgccctggcg atcctggtca ttttctttgc cctgtataac tacgcaggca acttctgcag
    34201 gtttggtgac ttttcttacg gcgtttatat tctgcacttc ccgattgtcc agaccctggt
    34261 ccatttccgc gcattcgagg ccaacccttg gctggccctg ctagccagtg taagcctgac
    34321 gctctgcgcg gccgcactga tgtggcatgg actggagaaa cgctggctgc tcaagaacag
    34381 ccactatgtt cgcgcaagtg cacaagatga ggcgcccgaa aaaaagcgcg aagcgcatac
    34441 cctttagtga atacgccagg gcctgagcag cgcggccagc gcggccatca gccccattgc
    34501 ggccagggtc gccacaccga gctgccacca gctattcacc atcatggcgg ggatcgccaa
    34561 agccaaagcg ggtaacagcc cccccagggt ccactgtcgt ggccaatcaa gtgccacgag
    34621 cagacgttgc ttacgcatta acaacaggcc tgtcagcaaa accccgcaca gcgccgccag
    34681 ggcgatcccc tttagcccca accaccaggg cagggccagg agcaacagcg cgttgactgc
    34741 attgccgctg agttcacagg ccagcggtaa gcgcgtatcg ccaaccgaat aggcataacg
    34801 cgccaacagc gcattccagg ccgcgaacag cagggtcagg ctcaaccagc cgagtagagc
    34861 ggccaatacg cctccaccgg cctggtttgg tagcagcagc agaaccagcg gctccgctgc
    34921 accgaccacc cccacggcca cgggcaacgt cagtagcgtt gccatcgccg taccacggcg
    34981 cagcaagacc agacgctcgc gctccgactt gccgcccatc aggcctagca gcacctgatt
    35041 caggctcatc agcgcaacca tcggcaggtt gatcagcttg cgcgcgaagt tcagccaggt
    35101 caccgcgcct tcaccgagca acgaggcaat catgcgttcg agcaacgcca atccctggct
    35161 ggccagagtg ctggcaagca aaggcccaag ccgctgcaac aattgttgcc cgcaactgcg
    35221 gtctgcctga acacgccagg ggcgccagcc cataccgcgc atggccggga agagcagcaa
    35281 cggcatcagc aaactgccca ccaaacagct cagcgcaacg cccgccggct cggcctggcc
    35341 acccttgaga gcgaggtaaa gcacaggcgg cagattgaat aacaacgagc caagccctgc
    35401 caggacgaag cgggcatggg cttgcaaggg tatgcaaagc agtgcatgaa gcataaagcc
    35461 cggaatacac cacgccagca ggtgaaggct ctgccaggca agcgcctgcc cctcagcgga
    35521 taacccagga ccgatcagcc atacccactg aggtgctgca agataaaaca gcccggcaag
    35581 tagcaaaccg acacccagca cacgccaggt cagagccgat aaccaggcct cgcgctgggc
    35641 gccctcccgt tcctgataca agggcaacgc tgcagagctg aaaagcccgg tggccaacgc
    35701 catacgcacg gcctcgggca aaaatagcgc gacgagaaag gcgtcactgc gcaggctggc
    35761 gccccatgca tcaatcagca gccattcacg ggcgaaacct gcggccagac caatcagggt
    35821 ggcgagcgtc agcatgagcg tagagctgag cacgatttaa cccctcacgg cgcattgccc
    35881 gccggtgcgc caggtagcac ctcggcctcc tcccgcaaac gacgcgcctc ttgcagattt
    35941 agcccgatca ttaaccagaa cagccccaca agaaccggct gaaaactgaa gtaatggtcg
    36001 aacagcccgc tgaacagcgc agccaacagg ccagcggtag taccaagcca gatggcattg
    36061 tcagcgttca ggtcgatgtt ccgggtacgc gggcgggttt ccttccacca gctccaggtc
    36121 gccgcgatga acagcaatag gcctggcagg ccgagcttgt agatgacgtt caaccacagg
    36181 ttggatatcc ccagcaaatc ggttcctgga actggtggct cgaccttaaa gccgataccc
    36241 aacgggtagc tggccactgc ccgagagaaa ttggcatatt cggcaaaacg cacggatgta
    36301 ctggcatcgc tgaactcgaa catggtcgag atacgctcct gcaacggcgg ataggacaac
    36361 accagcaaca gcccaaggcc acaaccgcag agcaacagac gcccggtaaa cggcacgcgc
    36421 cggatagcca tccagaccag caccaccgcc aggctcaaga gtgcgccacg tgaaccactg
    36481 agcagtagcg cgacgacacc gagcaaaccg accgcccccg ccaatgcgcg gcgccagcca
    36541 tgccgcgtga ggccgtagca gaaggccagt ggcagcacca aagccatggc cccgcccagg
    36601 gtgttggggt gcatccaggg cgagcccatg cgcagaaata tggccccggt tgtatcaccg
    36661 atcacctcgg ccagcgttgc atagccgagc ttggtcaaca gcgggttgat cgctaaagca
    36721 gagcgctcga caagaaagat cggcaaggat agcgccagca tcgccagggt accgagcatt
    36781 agcgccaaca ccatccagtc acgggacgcc ccatcacgca gcaagcgggc aaccagaaat
    36841 aacagcgaca tgttggccat ccaacgcagc cagttggcca cgccgttacc ttcggcctgg
    36901 accatcagtt gaccaaccac gaagggaaac gcgctgaaca ggatcaacgc cagcacgtag
    36961 cgttccgttg gaccggagcg ctgagcgtgg tccggctcgg tgaagagccc ctgaaacagt
    37021 acgcagcccc atatgacgag aatgagtaac tcggaaaccg tcgtccgcac cccgatattg
    37081 atggtcgagt agggcaggaa cgtggccacc gccgcgaaca gcaacagccc gagcaacggg
    37141 cggcgcagca ggttcaaacc gatgaccacg gcgacgatca ataccgccac gctcagtggc
    37201 gagagaaacc acagcagcac gccgaagagc aggccgagga ctaacgcaat cggtagccca
    37261 aatgaggatg tcatgcctgc aactcctgta gcagttcggc cactcgagct cggtaaggct
    37321 gctccgaata acgttcggcc catgctcgca aggcgtcacg atcagggtct tctggccgtg
    37381 ccaggcggtg catcagggtc accagggcgg caccatcata tggactgaag cgaggggcgc
    37441 caggcgctac gatttcatcc agagaactcc cagtggctac ggcaaccggg gtcccgccgg
    37501 ccaacgcttc aatcaccggc aagccgaaac cttccgccaa ggacggttgc cagaggcgtt
    37561 cggcagcctg gtaaacccct tgtaactcgg catcactgag cgccggcatc aggtgcaccc
    37621 caggtaaggc tcgcagcgcc gctggcagga actcaccacc accgaccaat accagttcag
    37681 gcaccgcggc gtccgcagca cgggccagat gccactgctc gagaaaccag ggcacgttct
    37741 tgcgtggttc gcgtgtaccg accagcagcc agaaacgcgc aggcagcggc agcggcggtg
    37801 caaccggtgt caaggtacac ctgggcacca gattaggcag aacccgaacc ttgctggccg
    37861 cccaggggaa ggtccgcgcc acctcgtccg cactgtattg cgacggtgtc cagatacgct
    37921 cggccacccg tatcgaataa cggattgaga gattgtcgct gagccgatat accgtttcgc
    37981 gcagccagga gctgtggcgg tttttcagat gcaactgaaa caggtcgtgc agttgcagta
    38041 cgtagcgcgt tcctgggggg cgcggcggta gcggcagccc catattgaag gtactgatgt
    38101 gcacctctat gcccaggcgc cgcaacgccc agggcatata ggccaactcg aagcgcagcc
    38161 gacgcggcag ccggtgttgc ccctctatag ggctggacca gctcggacaa tgcgcctgtg
    38221 cccgaatcgg atgatcttcc ggcgcggggg tgaataagct caactcgacg cctggtaggt
    38281 cgccgagcac cgatgccaat gccacgttct gacgaccgat gccattgttc ggattgctga
    38341 gccccggccg ataatccagc cctacacgca tgccacatcc ccctccgccc ggccagccag
    38401 gcaggtggca tagacccgct gtaattgctc ggccaccgcc atccagtcat gctcagcatt
    38461 gacataagca cgtccagcag cgcccatctg cgccgcagca agcgggcttg ccagttgcct
    38521 tagcacggcc agggccaggg cctcggcgtc atcccccccg tcatattcga cgccggcctc
    38581 cactgccagc ccagacacac cctgcgcagt cgtcaccacc gccaaccccg cagccatggc
    38641 ctccagcacc ttgagcttgg aaccgccccc ctggcgcaac ggcgcaaaga atagtgcggc
    38701 ccgggattgc agggccgtca ggtcggggac gaaaccgtgc cactcgatgc gcgtgtccgc
    38761 ccagcgatgc cgccaggcag gcggcagagc gtgccccgcg atggtcatgc agacatccgg
    38821 acgtgccgcc cagactttcg gcatcacctc atccagagcc cattcaaccg catccacatt
    38881 aggggggtat tcgtagttac cgataaacag caaacgggag ctgtcgtagg caggcatcac
    38941 cgtcgcatac cgcgcacagt cgacgccatt gaccactacc ggcagcacct tttgcagcac
    39001 ttgcgccaag gcctgggcgt cttgctcggt cactgcaata acttgctcgg cctgctgcat
    39061 cacccgcagc tcccaacgcc gatagcgcca ttgatcaagt atcaccagcg gtttgagcca
    39121 ggccggaaag cgatcatagg tagcgccccc caaggccgac tcaacattgt gttcggtgag
    39181 gatgaacggc tggccagcct gctgcagcgc ccgctcaaac ggctggaaac tgtaactatg
    39241 ttcgatctgc acaatgtccc aggtctcgtt caggagatcg gtgaagcgcc ggctcaagtc
    39301 aggagcgtag ccattgatgc tggccagcat aggtaacggc gaagcggcaa tagccaacaa
    39361 ggttttcggg ctacgcacag gtcggcgcgg caacacgatc agtcgctcaa gcagcggttc
    39421 cagggcctgt cggctgtcgt cgtccagcgc caccttgctc tgcaccagca gcgtaatgcg
    39481 atgcccagca agggccagtg cctgcagaag gttaaactga cgggtcttgc ccccgctgct
    39541 agttggccag ggtaggtagg gcaataccca gagaatcttc agcgacatca atcagtccca
    39601 gaccagtaat tgcaggctgg gcttgagtgc cacaaccaga gcacccgctt cagcctggag
    39661 cctgcgcgcc ttgccactat gtgggtcgtg cagggtcgcc tggcgaatgc ccggaagagt
    39721 caattttcca gcagaggcac tccacaggat gagcaaatgc ctgccatcct cccgcgtcca
    39781 ggcaatgcta tagaggtctt tcggagcgct accaaaagaa ggcgggtctg ccggtgtcag
    39841 gctcggccct gtcaccttga gaaagttctt caaggcccgg aaggccggtt ttggatcagc
    39901 attgaggtca agcaatccat atgactgatc acgcacgctg gcgcgcgcgt ccaggtcgct
    39961 caggttgaac aagaagatgc gatcgaaatc cagagcactc atcaatgcga gccggcgcag
    40021 ggtgtagtcg gcctgccccg acacaccaat gatggcctgc atttccttcg gtccggaata
    40081 gctggaccag ccccattcgg tcgcccagat ctgtttgacg ccctcgccgc gcaagccgct
    40141 gttgataaag cgcacggtat ccagcagctc attgtttccc ggcgagttgc cctcggggta
    40201 ttcggtgtac gggtgatagg ccacaatcag atctttctgc gccagcccct tcgttaacag
    40261 cgctttgagc atcaattcca tattgtgcga tggcatctgg ctgaaatacg ccataccggc
    40321 tgtgccgagg ggtttatcgg ggaatcgctg ccgtaccgta tcaaccgcat cgagcaagag
    40381 ggccgcatag gcatccgggt cctctttagg acgccaaaac gacggcaggt tcggctcgtt
    40441 ccaaacctgc caggcctcga cgctcgggta acgctcgacg aatcggctca ggctgtcgct
    40501 gtatagccga ttatccgtcg gcgggtagga gtcctgataa ggcgagcttg ccggggcact
    40561 ggtggcgaaa ggcgcggagc cggcgagaaa gccaacaggc ttcaagccat gctcggccat
    40621 cgctatgaca gaggcatcga acggagcgaa atactgccca ggcttgcgct caagcagcgc
    40681 ccaatgcatg gcaatacgta cccactgcaa gtccaattcc tgcaggcgag ccatctgctt
    40741 gaggtaagtg gcctcagaga agaaatgaaa ctgcgcatta acacccaggt agtctttcca
    40801 gaccacgctt ctgctcggtt taagactggt gatctcggcc tcggcatccc ggctactgag
    40861 tacggcagcc ccgagaagca cgcacgcaac aacggtggcc aatccgataa aacgtctatt
    40921 gcgcataatc tctcccctcg cacagagcag tgaacaaggc cttaaagcgc tgcgcggtca
    40981 caggccagag ccgctcttgc ccaatagcgg ccgctcgctc ggcccattgc tggcagcgct
    41041 cgggctgctc cagtagagac tgcaattggc tagccaacgc cgcgacatca ccttgggcga
    41101 atatgccacc atttcccgtc gacacctctt cggcaaaagc ccgcgcgtcc gaggtgataa
    41161 caccgcggcc acaagcggca gcccaggaca aagcgccact ggtcccccgc tgtgcgccaa
    41221 gcaacctcag tttcttcgac tcgcggtacg gaagcaccac cacatgatga gcctgaattg
    41281 tctcagcgat aacctcgcta ggtagatcca ggcgccactc gatcagtgat tcaagcccca
    41341 gctcgacaat ccgtcgacgc aactggtcga tataactttc tcctgcgtcg aaggccattt
    41401 ccggtgcgct accgccagca agcgtcagac gaacgcgttg ctgcaactgg ggctgctggc
    41461 ggatcaccag ggccagggcg tcaagcagat cctcaatgcc cttgccacga taaatgaagc
    41521 caaaatagag caagcgcagg ggctctagag gcggcagagg ttgcgccgca atatgctggt
    41581 tgccgtgcgc gatcacctgc acccgctgct caggcagacg cattcgccgg gtcagacagt
    41641 tacgccctag agcggtcagg gttaccagag catccaggct attggccagc cgccgctcct
    41701 catacaaaca cagcgggtcg gccagaaccg ttgccgcctg cggccagggt gaaggccacc
    41761 tctgggttag cgacaacggc cagggcaaag atgcacggcg ccacaccaac cgctccgggt
    41821 catgcacggt ggcggtcaag ggcaactgcg caaagcgctc acgcagggca cgcagggcgt
    41881 ggaactcggc caagcggcca ccgcccagct cggcatgcac cagcgccacg ccagaccagt
    41941 caaaggttct cacgcgctcc agcgcgcaca gagggtcatt gccacacccc gccagcggcg
    42001 ttacaacctc gacgccgaca gcctcgagcg aaacccgcaa ctgctccgca taatccgcaa
    42061 tgccggtctg ctcaggaggc aacggcgcca gcagggcgat tcgcatcaga tcacgccccg
    42121 aaccttggcc atcatccgca gcacacgatc aggcacttcg aaacgacgcc ggttgataat
    42181 aactccgtca accttgctga agccggtatt cagtacttcg atagcatgcc ccaccgtcgg
    42241 cacattactc tgctgagcct cgaccaccag cgcgacaagg tcagcctggc gcaggctgac
    42301 gaatgcctcg gggttggaca gcagtgccga tgcatccagc aacaccactt cgccaggctc
    42361 tgcgggggct gggccgatgc gcaccttgtg cccacgctct tcgagtagga gctgcagatg
    42421 gccaacaata aagctgaccc cctcaccacg ccgggacgag gtcagcccca gcaccaaccc
    42481 ccgttcggcg acctgttcca gcggcagcat gccgtatagg cgatagagcg cggccaaaaa
    42541 ggcgctgctc tggctcgact ctgcggtaat ttccggcaac gtggtccaca acggaatacc
    42601 gaacttgctg gcgaagcgac caccgtcgtg aatacgctgg tccagcaggt aacacaggta
    42661 aaccaccagg aaaccaacca ggatgctcag cggcagcgcc agcaggagca tcaccaaact
    42721 tttggggaaa acccggctcg ggttgtaggt ggcctgctca atcatcgcga tattactgat
    42781 gcggctattg tccagctccc ggtcaatccg cgccttctcc aggctttcgt tgtacagcgc
    42841 gaaattcttc tcggccgcag tcaattcacg ctgcaaacgc gccagatccg gctcgacgga
    42901 caaggcctta ccccgatcct gttcaagtag cttgagctgc tcatcctggc gcaacagttg
    42961 ggccttgagc cgtgcgagat ccgcccgctg atccatgaaa ttgctgcgca gacgctcggc
    43021 gatcggatta ggtgcctcgt tctgagaaac ctgcacagta gcggactcgg ccttcaccat
    43081 cgcttctaga ctggcaatgt tctcatcaat actctgcact ggcggagcat tttccgtgaa
    43141 ggtccgcagc aaatcctgac gctcggcgcg cttgttattg agcatgcgca ccagatcttg
    43201 atgaatcgga ttgagggcca tctctcggac agtacgaact tcggccggca ggctagcgat
    43261 ttgctgctca agagtggcaa ggctaccctc ggtggaagaa atgagccgcc acgtattgaa
    43321 acgatcacca cgcagttggt tgatacgctc tgacaggttc tgcagatgcg cctcaatacc
    43381 cacagtgccc agttcattta ggcgtacgcc caattcctgc ttgtacttga gaatatcggc
    43441 agcactggct tcactctggg tcagatagaa ctcgtagaga ctacgccgcc ccagcgcccg
    43501 agtacgctcc tccaagtaaa gctcaaccca gcttttgacc accgcttggg ccaatgccgg
    43561 atcgctccag cggaaacgga tttccatgac tgacgagcca ggggcatggg tgacgctaaa
    43621 gtttttttcc aggcgcgcag ccagccggtc taccggcgtc tgttcctcag ctaggccaag
    43681 cagctggacc acgctgcgta caagcccaac cgcaccactg acgacctggc caacatagta
    43741 tttaagcgtg gcgaagaacc cctcaggacg cggcgtattg gccaacgcct ccagatagtg
    43801 ctcggcaacc tggcggctga taggccggcc agtgagcatg cgttcttcat ccagaatagg
    43861 gtcgcgctga gtggccggca tcacgatgga ctgcctgtca gccacttcga tcggcagggt
    43921 gttggtgtca cgtcctggct ttaccaacag gcgcgcgttg gactcgtagg aggtcggcag
    43981 caggaacgcc cccaaaacga ccacgaccag cgtagcaatg accgccagtt tgaattcctg
    44041 gaaaaagatg aaaaacaggc gtagcaaatc acggaatgtc cgaatctcga tcatgatggc
    44101 tgtcctcagc gactagttgc tgttgccacg gaggtcataa gaagccccga tgccaatgga
    44161 tttaacaaag gggatcagtt gattgaggta gaggtcgacg ccctctatgc ggttccccac
    44221 acctgacttg ggtacaaaga ctacgtcgcc ccgctgcaac gcgacagcct gacgcccttg
    44281 ctcgccgccc tgtagcagct ggctgtagtc gacgaagtag gtcttgtaac ggccatccac
    44341 gtccgagcgc agcagcgcga catgccgact atcggcatca ggtagaacac caccggcggt
    44401 gacgatagcc tgctccagcg tcgtgacgac attcgtcgag tacggctgag ggttacgcac
    44461 cgaaccacca acgaataccg tgttgccagg tgcctgattg atattcacgg taaccccggg
    44521 ttcgcggtaa tagggggtca agcgctcgcg cagctcatta cttaactgct caggggtttt
    44581 tccgcccgcc tggaccttac cgatgaaggg ataggagaag ctgccatcat tcatcacggt
    44641 atacagcgta agctcgtata cagagttggc cgtatacgca gagatcgtgg gcgtctcgcc
    44701 actggcacgg acgacacgca atgtgtcgcc aggatagatt cgctcctcga ccgcaggcag
    44761 atcggccagc ttcagcgcct cgacctggcc ggcctccagc gtatcggaat ctggcaaggc
    44821 aatacgcgca ggcgtcgtgc aagccaccag gccaaacgtc ataacaagta gcaaaaacac
    44881 tttcataacg ggaattccct ttaagacctc aatcttcact tccagtaccc cggaaacatg
    44941 cttatgcgcc gctttatccc tgtatcgaga aaacgctcgc cctgaccgat gcgataaccc
    45001 aacaatttca ttgcactgcg tagcagcact tccggcaacc ggtgcaacgc tccggcctcg
    45061 cgaagcgcgg cgatctcagc cagcacatag cgcttgccct cgcctccagc actaccaaac
    45121 gcctggccaa tccagctctc acgcccgtaa aacacaccga tatcgaaata gcggcgaaac
    45181 tcttcgagca cccgataatc atgggaatgc tggacctcgg catctgctgc gtaacgcacg
    45241 gcgtagccgg cgagcagcag acgcgcagcg acataagcat cttcactgcc gatcacatcc
    45301 tcgggaaaac cgccaacagc gcacagcgcc tcacgtcgat aggcggcaaa ggagtcggag
    45361 ctgaaacagg ttttgatccc caactcggag gcatcccgaa gccgcttggt acggctgact
    45421 ggcggatagt tgaagcgacg cgactgggcg cccaacagac cggcatgcgg atgcggtaac
    45481 tgacggccat aggcgacacc gatatctggc tcactctgca aatcactaac aatgcgggcg
    45541 aacgtctcgg ggctggccgg gatagcgtct tgggtcaagt agatcagcac atcggcgtct
    45601 acctgcgcgc tggcccagcg tcgcgtgcca ccatggttga actgggcagg ctcaatcacc
    45661 tcaacccgcg cgccggcgtc gcgaaagcgc accacgctgt catcacgcga agcactgtcg
    45721 acgaccagaa attcgtccgg ctgcaaggtc tgcgcagcaa gcgccggcag caaacgatca
    45781 agatgggcag cggcattgcg ggtcggaatg atgagggcca gacgcatcag atggccttct
    45841 cggctgcagg cacacggccg tatatatcgt tgaagcgcac gatgtcgtcc tcaccgaggt
    45901 actcgccgct ctgtacttcg atcatcacca ggtcaatcac cccaggattg gctagacgat
    45961 gagtatgtcc ggccggaata aaggtcgatt cattggtatt gagcaggaaa tcaccttcgc
    46021 cattgaccac gcgagccatg ccactgacca caatccagtg ttcgctacgg tgatggtgca
    46081 tctgcaaaga cagcgactca ccagggcgaa ccatgatgcg cttgatctta aagcgcgacc
    46141 cttcctcaag aacgctgtaa gtcccccatg gacgggtcac cgtacggtgc agacgatagg
    46201 cgtcatggcc gcggcgcttg agctcctggg caataaactt gacctcctgg ctgcgctcag
    46261 cattagcaat caacaatgca tctggcgtat cgaccacaat caagttatcc aacccaacca
    46321 tgccaacgag acgcctgggg gagtcgatgt aacagttatg cacgtcgtgt aggacagctt
    46381 caccgataat ctggttacct tcgctatcgc gcgggcttag ctcacgcaag gcctgccagg
    46441 aaccaatatc actccagccc aagttacagg gaacaaccgc tacacgatcg gagcgctcca
    46501 tcaaggcgta atcgatagag atatctgggg ccaacacaaa ggattcactg tccagttcac
    46561 gctgacacag tttattgcct gagagctcgg ggcttcttgc taagcagttt tgtacagccg
    46621 ccagcacatc gggagcgaac tgctgaaact gcgccagcag agtatcgacc tgcagacaga
    46681 acataccggc gttccacaga tactggccgc tttttacgta ggcctcggca gtcgccaagt
    46741 ccggcttttc aacaaagcga gaaacggcat aagccccggc cacaagcggt tgtccttgct
    46801 cgatatagcc aaaccccgtt tctggatgct ccggctgaat gccaaaggtt accaaatagc
    46861 cttgtgatgc cagttcacgg gcgctggcca cagcctgggc aaaagcctgc tcatcccgaa
    46921 tcagatgatc agccggaagt actagcaagt gcgcgtggtc accatactgc tgctggacat
    46981 gcagcgcagc tacggcaata gcaggtgctg tattgcggcc gaaaggctca agcaggaatt
    47041 ccagtggcaa atcctgctga ttaaccgtgc ggtagtcatc caatgtacgg aacagcaact
    47101 cacgattcgt caccgtcagc actcgctcaa cgccaggcaa ttggccaacc cgcgcaaagg
    47161 ttttctgcaa cagactctga ccatcgggga gccgcataaa tggcttgggc atggcctcac
    47221 gggataccgg ccacaacctt gtacctgcgc cgccagcaat gatgcaggga atcaactggc
    47281 tcatcgattg ctccattagt aagcctcctc ggtcaggatt agcgctggca acgtcctgaa
    47341 taaaatgtac aggtcgaacc acaccgacca actctcgatg tactccaagt cgtattccac
    47401 gcgtttctcg attttttcca gagtgtccgt ctcaccccga taaccattaa tctgcgccca
    47461 gccggtaatt cctggtttta cacggtgccg agcgctgtac tgctccacgg cctcttcgaa
    47521 cagcacgcct gctgctttcg tggcggtggc atgcggacgc ggccccacca tcgacatgct
    47581 gcccaacaac acattgaaca gctgcggcaa ctcatccagg cttgtcttgc gaataaaccg
    47641 gccaacacgc gtcaccctgt catcattacg tgttgtctgc cgctctgcat tagcgtcgct
    47701 ctggtgataa aacattgagc ggaacttaaa tacctctatc aagcggttgt tatagccgta
    47761 tcgattctgc cgaaacaaca caggccccgg cgaatcaagt tttatgacca ctgcaagcag
    47821 taacatcagc ggcaacagcg cgattatggc cagccccgcc aacattaaat cttctaaacg
    47881 cttaatcaac ggcgaccaac ccgatagcgg tattttcgat acattgaaca tcggcaaacc
    47941 gcaaacagtg gtgatgcgat tgtgcgcatg ccggaatgca accatgtctg ggaccaacaa
    48001 tacattcaca ggcaagcggc gtaagtcacg aacaatctgt ccaatacggt tctccgcaga
    48061 ccagggcaga gcaaccagca cctgagtcac tcgctcatca cggatcattt tctccaactg
    48121 acggctatta ccgatcactg gcaagcctgc cagggtttca ggcatccggt ctatccgatc
    48181 atctataaag ccaagcagtc ccgagcggat gtcttgtccg cgcaacaaat attcggctag
    48241 ccggacaccg ttttctgtac cgccaacaat caccgcagtc tgcaaaaaat gcccgcgcct
    48301 tatcaaataa cggaaaacca ctagcagcaa caaacgttcc acgccgaagg cggcaagact
    48361 aaaagagaac cagaacacca gaaaaggtaa tgaaatataa cgaaacagct cgagagcctg
    48421 atgcataaat aacagtagac aaaacgcagc agtccacgca aaaaacatga cgcgaaagcg
    48481 caaacgatta ctgaatattt cctcagagta aacacccaac gcttgaaaca ttaaaacgct
    48541 taacaaccca aagaataata aaaaaccgaa atactcatgg tttaaattaa ctgagctatc
    48601 ggttaacagc cacaacaata taaacccagg aatacttgca gtagccccat gcacaatacg
    48661 aattgccgcc aggaaatatt caataaatcc tggtcgcgct aaaagcagac tatctacctg
    48721 atgcttacgc acaagccctc cccccccccg cttacccagc ccaagccaga acagctaaag
    48781 acaaacacaa aacgtcaaat taacgacaca gctcctcctg cgaaatggtg aggacttaag
    48841 catgattcag gtagtcactt caatccaaat catggctatt agccataaat aaatgtcatt
    48901 ttaatggcgc catgtttttt cttctgcgca ttctagaaag atcacgctcc agagctgagc
    48961 gtacttaaga aaaaatgact aattagagtg cattggcaaa acaactattc atgacccacc
    49021 acaaagcaac gcacgccagt tgcatagtgc ctcgtcaata cttggccagg atggtttatc
    49081 gctaattttc ctataccatt gaccaccaac tacggcacaa cccatgcaac tggcgtgccc
    49141 cgccgggatc tcttcgagtt aggccattaa tatgcatatc cttattaccg gcggcgccgg
    49201 ctttatcggt tcggcgctga ttcgccactt gttgaatcac accgattatc aggtgctcaa
    49261 cctcgacaag ctgacctatg ccggcaatct ggagtcgttg gccagtgtgg cgggccatcc
    49321 gcgctatcgc tttgtgcagg cggatattgc cgacagcgca gccgtcagcg agacgctcgc
    49381 cgagtttcag ccggatgcga tcatgcactt ggcggctgag tcgcatgtcg accgctccat
    49441 cgacggcccg gcggcattta tccagaccaa tattgtcggc acctatgcgc tgctcgaggc
    49501 aacccgcagc tactggttgc agctggagcc cgcgcgcaaa gcagcatttc gctttcacca
    49561 tatttccact gacgaggtgt atggcgacct gcatggcgtg gacgatctgt tcacagaaac
    49621 cacaccctat gcgcccagct cgccctactc ggcaagcaag gccgcgtcgg atcatctggt
    49681 gcgcgcctgg cagcgcacct atggtctgcc ggtactgatc actaactgtt cgaacaatta
    49741 cggcccctac cacttcccgg aaaaactgat tccgctgatg atcctcaatg ccctggatgg
    49801 caagccacta ccggtatatg gcaatggcca acaggtgcgt gactggctgt atgtggaaga
    49861 ccatgcccgc gcgctattaa aggtagtgag cgaaggcaag gtcggcgaaa cctacaatat
    49921 tggcggacac aacgagcaga aaaacctgca tgtggtggag agtatctgcg cactactgga
    49981 cgagctgtac ccacgccaga acggctccta taaagagcag atcagcttcg tcactgaccg
    50041 cccaggccat gacctacgct atgccatcga tgccagcaag atcgaacgcg agctgggctg
    50101 gaaacctgag gaaacattcg ccagcggcct gcgcaaaacc gtgcgctggt acctggataa
    50161 cctcgactgg tgccgccgcg tgcaggatgg tagttaccaa cgcgaacgcc tcggcgcact
    50221 ttaaggagca gcacgatgaa aggcatcatt ctcgccggcg gttccggtag ccgcctgcac
    50281 ccgattaccc tgggcgtgtc caagcagctg ctgccgatct acgacaaacc aatgatctac
    50341 tacccgatct cggtgctgat gctggcaggc gtgcgcgaga ttctgattat ctccaccccg
    50401 caggacttgc cgaattttcg caagatgctc ggtgatggca gtcaatttgg tgtgcgcttc
    50461 gaatacatcg aacaaccttc acccgacggt ctggcccagg cctttattct gggcgaagaa
    50521 tttatcggca aggattcggt gtgcctgatc cttggcgaca atatcttcca cggccaacac
    50581 ttcaccgaga aacttctacg cgcagctgcc gaaccctcgg gcgctaccgt tttcggttac
    50641 tgggtgaagg acccgcagcg ctttggcgtt atcgagttcg acgcccaggg caaggccatc
    50701 tccatcgagg aaaagccgac agtaccgaaa tccagctacg cggtgaccgg cctgtatttc
    50761 tacgacaacg atgtgatcga gatcgccaag gcagtcaaac cttcggcacg cggtgagctg
    50821 gaaatcaccg acgtcaacaa cgcttacctg caacgcggcg acctgcgtgt agagcgtttc
    50881 ggccgtggct ttgcctggct cgacaccggc acccatgaca gcctgctgga ggcctcgcaa
    50941 tacgtgcaga ccatcgagca ccgccagggc ctgaaagtcg cctgcctgga agaaatcgcc
    51001 taccagcagg gctggatcga tcgtcagcaa ctgctggcac aggctaaagc ctttggcaaa
    51061 accggctacg gccagtacct gttcaaactg gccgaggaat gagcatgcag gctgttagca
    51121 ccgacctacc cggcgtgatc attctcgaac ccaaggtgtt cggtgatcaa cgcggtttct
    51181 tcttcgaaag tttcaatgcc cgcgcattcg ccgaggctac cggcctgcag cgcgagtttg
    51241 tccaggataa ccactcgcgc tcagccaaag gcgtgttacg cggcctgcat taccaggtgc
    51301 agcaggcgca gggcaaactg gtgcgagtca ccgccggcga agtctatgac gtggccgtgg
    51361 atctaagacg cagctcgcca accttcggca aatgggtggg tacccggttg tccgccgaaa
    51421 acaaccgaca actctggata ccagagggtt ttgcccatgg ctttgtagta ctcagcgagt
    51481 tcgccgagtt tctctacaaa actaccgact actatgcgcc ggaacatgag cgctgcatcc
    51541 gttgggatga cccaaccctg gccatcgact ggccactgga aaacgcgccg cagctatccg
    51601 ccaaggatca ggccgggctc agcttcgccg acgcgcaggt cttcccgtga agattctcct
    51661 gctcggccag cacggccagg tcagtcagga attacagcgc agcctcaacg ggcaagccga
    51721 gctgacagtg ttgagccgcg aacagttgga tctggctcag cccgaactaa ttcgccagca
    51781 cgtacagcgc ctgcgccccg agctgatcat caatgccgcg gcgcacaccg cagtggatca
    51841 ggccgagagc gaaccggagc tggctttcac catcaacgcc accgcgcccg gcatcctcgc
    51901 cgagctagca gccgagctgg gcgctccgct gatccactac tccaccgatt atgtgttcga
    51961 tggcagccac accgagcctt accgcgaaag cgacgccacc cacccgctga gtgtttacgg
    52021 acgcagcaag cttgctggcg aacaggcgat tcaggccgtg ggcggtatgc acctgattct
    52081 gcgcaccagt tgggtctact cgcagcatgg caaaaacttt ctgctgacca tgcaacgcct
    52141 gctgcaggaa cgcgaaagcc tttccgtggt ggacgatcag atcggcgccc cgacctgggc
    52201 cggcactatt gcgcaggcca cggcgcagct gatcgagcaa tggcgcagcg gccatgccgg
    52261 cccatggggc gtgtatcacc tgactgcaca gggcgaaacc agctggttcg gctttgccag
    52321 cgcgattgcc gagcacttgc gcagccaggg caaaagcgtc gcgacgctgc aacccatccc
    52381 cagcagcgcc tacccaaccc cggcgcagcg tccgcttaat tcccggctgg actgcagccg
    52441 cctgcagcag gactggcacg tccaattacc ggattggcaa gccgcgctgg cgcaatgcct
    52501 agcccgcagc taagcccgaa agcgctgaat ctgctgctgc aaaccgctgg ccagttgcgc
    52561 cagatcgcga ctggcaatcg aactctgctc ggcgccattg accacttcgc caatgctgtc
    52621 gttaaggctg ctgatgttct ggctgatctc attggccaca gccgtctgct cctcggtggc
    52681 ggcggaaatc tgcacattgc cgtcggcgat gccctcgatg gcatgggcaa tatgattaag
    52741 cacttcaccc gcctcgcggg catggctgac actgttctgc gcctgctcgc gcgatagctg
    52801 catggcgctt accgcactgc gcgcgccacc ttgcagggca tcaatcatgc tgcgaatcga
    52861 ctcggtggct ttctgcgtat tgctcgccag gctgcgtacc tcgtcggcca ccacggcaaa
    52921 gccgcgcccc gcttcaccgg cccgcgccgc ctcgatagcc gcgttgagcg cgagcagatt
    52981 ggtctgctcg gcaatgctct ggatgacacc aagcaccttg ctgatttcat cgctctggct
    53041 gtgcagggta tcgatcacct caccagcttt ttccaccgac gccgacagcc cctgaatcga
    53101 cttgaggttg gactgcacca attcactgcc ctgacgcgct tccttattcg cgtccgccgc
    53161 attgctcgca gtggactgcg cattgcgcgc cacctcctgc accgccacgc tcatctggtg
    53221 cacggcggtg gccaccatac tggtctgatc gccctgggcc gtagcggcct ggctgacctg
    53281 agcactgatc gcactcattt cctcggacgc cgcagccagc tgagtcgcgg tatcggtcag
    53341 atgacgaatc aacgcctgct ggtgttcgag catgatattg aaggcccgcg cggtatcgga
    53401 cacttcatca cgaccgctgg catcggcgcg cagggtcaga ttggaggact gctgcacttc
    53461 gctgatcagg ctgcgcaaac cggccagcgg gcgaatgatc gacaggctga tcaacagcgc
    53521 cgccaccagc accagcacca gcgccaccgc gccaatcagc agaaaggtgg tgcgcatcga
    53581 ctggtactgc gccgtagttt ctgcgttaag ccgctcgcct tcgcttaact gcaggctgat
    53641 taggccttcc agttcgccgc ccagcgggtc gaaggtgtca tataggatgc ggttgaacgg
    53701 gccagcttcg gctgccaata actggccatt gccgagctgc gccaggtact gatcggccag
    53761 ttgctgcacc cgcagcagat gcggcttggt gcgctcgact cggttgcttt catcgctggt
    53821 caggcgggtg gcaatgtact cggcccaggc cttgtcgcca cgactacgtg cctcgctgaa
    53881 cgccttgcgc aacgttgact catcaaatag cccggcacgg tacttatgca gcgcatcgac
    53941 catcgccacc gcgtaggcat cggacaccac cttgagctgg ctgatcggct gcatgcggtc
    54001 ggtaaacagc tcgtcaaagc tattgttaag cttgctggcg ttggataacg ccatgccgat
    54061 gaccacaatc agcaccagca gcggcaccac cgccaatagc aacaacctcg cccttaccgt
    54121 caattgagcc agcatcagga tatctccagg gactaaagcg cagccgtcca tgctgggcgc
    54181 aaccgggcgg caccattact ccagggcgat gccgaatttt ttgagtattg acccatatcg
    54241 gcgagcttgc ccacttattg cataaccgcc actctgaact gattaccctg cccgagtaag
    54301 cgcatgccca ccgcccaccc tcacccgcgt cgcccacgct ggcgcagcct ggccttactg
    54361 gccctgctgc tgacgcccct gctgtggccg ctgcagcaac tggccgagcg ctactacagc
    54421 ggcgtgctgg tcgaacaaaa ccgtcagacc ctcgacctct acgtcgccaa cctgctcggc
    54481 accctgcgcc gttatgaagt gctgccaccg attctcggcg acctgccggc cctgcgcctg
    54541 gccctgcagc agcccagcga gcgcgccttg ctggagaacg ccaaccgcct acttgaccag
    54601 gtccgcgggc aaaccggcgc cgacgtgatc tacctgatgg accccaacgg caaaaccctg
    54661 gccgcctcca actgggcgca ggccgactcc ttcgtcgacc gtaatttcgc ctttcgcccg
    54721 tactttcgcg aagccatcgc cggccgcccc ggacgtttct tcggcctcgg caccacctcg
    54781 ggcaagcgcg gctactactt cgccgctgcc gtgcgcgacg gtgagcgcag cctcggcgct
    54841 ctggtggtca aggtcgacct cgatcacacc gaaaccctgt ggggcaacac cccggaacag
    54901 ttgttggtca ccgacgccaa tggcgtggtg atcctcacct ccaacagcca gtggcgcttt
    54961 accgccagcc gcccgctgga tgctgccgag cgcgccgcaa ttgccgccaa cctgccctac
    55021 ccaacccagg caccaccgcc gatcaagctg gatgcgcaga actggattcg ccaggaccgc
    55081 gaactcaagg aaaccggctg gaccgtgagc atcctcgccc cacgcagcct actcgaccgc
    55141 ccggtacgca ccgccctggt ggccggcggc ggcatgctgc tgttgctgat gctgctactc
    55201 ggcctgctga tgcagcgccg ccgccactac ctggaacgca tcgccctcaa tacccaggcc
    55261 cgccagcagc tggaacaacg cgtactggaa cgcaccctgg acctggaacg gctgaatagc
    55321 cggctgaaac aggaagtgct ggaacgcgaa caggcgcagc aggagctggt gcaggcccag
    55381 gatgaattgg tgcaggccag caagctttcg gccctgggca ccatgagtgc gagcatcagc
    55441 catgaactca accagccgct ggcggccatt cgcagctacg ccgacaacgc ctgcgtgctg
    55501 ctcgaccacc agcgcggcga tgacgcccgc ggcaacctca agctgatcag ccagctcacc
    55561 gagcgcatgg cctcgatcat cgcgcacctg cgcgccttcg cccgccgcga ccgtcacgcc
    55621 ccggaaagcg tggccctgca gccggccatc gacgatgccc tggccctgct ggccaagcgc
    55681 cgccaggcca tgcaagtcga gctgattcgc gacctgccga acgccaccct gtgggtacag
    55741 gccggggaaa ctcgcctgcg ccaggtgctc ggcaacctgc tggccaacgc ccttgatgcc
    55801 ttaagcgaag tgccgcagcc gcgccggctg tggctaagtg ccgaacttac cgccgacggc
    55861 gtcgatctac acctgcgtga caatggcccc ggctttagcc aggaggcgct gcaacgcgcc
    55921 cgcgagccat tcttcaccac caaaaccagc gctcagggcc tcggcctcgg cctggccatt
    55981 tgcgataccc tgatgcgcgc cctgggcggc gaactgctgt tcgccaacca ccccgacggc
    56041 ggcgctcagc tgaccctgcg cctgcgcgcc gcggaccagg gcatcaaaac ccagtcgcca
    56101 gaggactttg ttgtatgagc atgatcgact cccgcaccca ggtgctgctg atcgacgatg
    56161 acccgcacct gcgtcaggcg ctgagtcaga ccctcgacct cgccgggctc aaggtcagca
    56221 gcctggccga tgcccatggc gttgccgcgc gcattgagcg cgactggccg ggcgtagtgg
    56281 tcagcgatat ccgcatgccc ggcatcgacg gcctgcaact gctgcaacag ctgcaggaac
    56341 aggacccgga actgccagtg ctgctgatca ccggccacgg cgatgtgccg ctggcggtgc
    56401 aggccatgcg cgccggcgcc tatgactttc tggaaaagcc cttcgccagc gacgatctgc
    56461 tcgacagcgt gcgccgcgcc ctggccctgc gccgcctggt gctggataac cgcagcctgc
    56521 gcatggctct ggccgaccgt cataccctat cggcgcgctt ggtcggtcag tcgccggcga
    56581 tgttgcgcct gcgcgaacag atcggcgccc tggccgggat caacagcgac gtgctgatcc
    56641 tcggtgaaac cggcgccggc aaggaagtag tggcgcgcgc gctgcacgac ctgtcgagcc
    56701 gccgcgccgg gccgttcgtg gcgatcaacg ccggcgccct ggccgaatcc gtggtggaaa
    56761 gcgagctatt cggccatgaa cagggcgcct ttaccggcgc gcagaaacgc cgtatcggca
    56821 agttcgaatt cgccaatggc ggtacgctgt ttctcgatga aatcgaaagc atgagcctgg
    56881 aggtgcaggt caaactgctg cgcctgctgc aggagcgcgt ggtcgagcgc ctcggtggca
    56941 accagttgat tccgttggat atccgggtga tcgcggcgac caaggaagac ctgcgccagg
    57001 ccgccgatca aggccgcttc cgcgccgacc tgtattaccg cctgaacgtc gcgccgctgc
    57061 gcatcccagc cctgcgcgag cgcggcgaag atgccctact gctgttcaag cactttgccg
    57121 aagccgccag tagccgccat ggcctggccg aacgcagcct gcagccaggc cagcgcgcgg
    57181 cattgctgcg tcacccctgg ccaggcaacg tgcgcgagct gcagaacgcc gccgaacgct
    57241 tcgccctggg cctggaactg gacctcgatc tgcaggccca ggccgcggca ccgatgcctg
    57301 atgaagacgg caacagcggc ggtttgaatg cccaagtgga agccttcgag cgcagcctga
    57361 tcagcgccga actgagccgt tcgcacagct cgctgcgcag cctggccgaa gccctgggcg
    57421 tgccgcgtaa aaccctacac gacaaactac gcaaacacgg tctgagcttc gccgatgctg
    57481 gcggaagctc gccagacagc ctcgactaga cggcggattt ccgccaaaag ctgtgcaaac
    57541 ggcctgcgca gccactcgcg aaatcttctg caacaactcg ccaggccttg tcgctgctgc
    57601 gtttcacaat actcgcagcg accatttcag tgcctggcat ggcggttgct ctagcctctt
    57661 ataggcgatt cacaaacgcg cagatctagg caatcgctgc cagcgtccgt cattttctga
    57721 caggccgcct agacttgagc ctgttcgccc gatttgtagc gctgcggcgc atcacgctat
    57781 acaaaaacaa gaggaaagac tccatgttca aactgactgc caaggcgctc gcgtgcgccc
    57841 tgtcgctgag catcgccggt atggcccaag cggccgaccc gatcgtgatc aagttctccc
    57901 acgtggtcgc cgaccacacg cctaaaggcc agggtgccgc gctgttcaag aagctcgctg
    57961 aagagcgtct gggcgacaag gtcaaggtcg aggtttaccc gaactcctcg ctgttcggtg
    58021 acggcaagga aatggaagcg ctgttgctcg gtgacgtgca gctgatcgcc ccatcgctgg
    58081 ccaaattcga gcagtacacc aagcaaatcc aggtattcga cctgccgttc ctgttcaagg
    58141 acatggccgc cgtcgaccgt ttccagcaaa gcccagaagg tcaggcactg ctgaaatcca
    58201 tggaagagaa aaacatcact ggcctgggtt actggcacaa cggcctcaag cagctgtccg
    58261 ccaacaaggc gctgcgtgag ccgaaagatg cccgtggcct gaaattccgc gtacaggctt
    58321 ccgccgtact ggaagagcag ttcaaggccg ttaaagccaa cccgcgcaag atgagcttcg
    58381 ctgaggttta ccaaggcctg cagaccggcg tggttaacgg tgcagagaac ccctactcga
    58441 acatctacag ccagaagatg cacgaagtgc agaagttcat caccgaatcc aaccacggtg
    58501 ttctggacta catgctgatc accaacacca agttctggaa cagcctgcct gccgacgtac
    58561 gcagcgagct ggacaagatc atcgtggaag tgactgctga agtgaacaag caggctggcg
    58621 cgctgaacga tggcgacaag cagcgcatca tcgacgcgaa aaccaccgaa atcattaccc
    58681 tgacccctga gcagcgtaat gaatggcgcg atgccatgaa gccggtgtgg agcaagtttg
    58741 aagctgaaat cggtgctgac ctgatcaagg ctgccgacgc agccaaccag taaggcggag
    58801 caggcctcgg cctgaaccca accacagcgc agccttgccg ctgcgcgatt ggccgaatac
    58861 ccgggcaacc cctcgcgggt tgcctgggta cgccgcccct caccactgga gactctatcg
    58921 atgaacgcct tgcggcgcgt ctgggaccac ttcgaggaag gctttatcgc gttcctgctg
    58981 gccgccatga cgctggtgac cttcgtctac gtgatcctca a
//
LOCUS       DASRUM010000177        14772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1094450, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000177 DASRUM010000000
VERSION     DASRUM010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 14772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14772
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094450"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /gene="cheR"
                     /locus_tag="VFF22_11510"
     CDS             <1..811
                     /gene="cheR"
                     /locus_tag="VFF22_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HZX17477.1"
                     /translation="DFEQFRTFLEKACGILLGTNKQYLVSSRLNKLMEQNSIKTLGEL
                     VQRMQSQPRSGLREQVVDAMTTNETLWFRDTYPFEVLKNRVLPEMIKASPGQRLRIWS
                     AACSSGQEPYSLSMTIDEFEKTNIGQLKGGLQIVATDLSPTMLINCKSGEYDSLAMGR
                     GLSQERLQRYFDPKGPGRWVVKPAIRSRIEFRPLNLLDSYASLGKFDVVFCRNVLIYF
                     SAEVKKDILTRIHAMLKPGGYLFLGASEALNGLPNHYQMVQCSPGIIYKAK"
     gene            complement(881..3964)
                     /locus_tag="VFF22_11515"
     CDS             complement(881..3964)
                     /locus_tag="VFF22_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZX17478.1"
                     /translation="MGAPWKLDITTLPAGDQPRPSKRTSKLKVTFWLLVLLLCIAAGF
                     LLVEEVRTSRFQAQELSRYAQTLGYRVKPGPSPSIIYPKEGPFDKRQGYTYLPLMLER
                     LDQRGFTVQQQAQFSPALLDYAQRGLFVPYPEKIQSGLRINDCSGTPFYEFRYPQQRY
                     PSFNAIPPLVADSLLFIENRKLLDPEQPLANPAVDWPRFVMAAISQVGKLLDMQDQSA
                     GGSTLATQLEKYRHSPDGLTLSATEKLRQMLSASVRAYQSGPQTLAARENIVRDYLNS
                     VPLSAAPGHGEVHGLADGLRIWFGADFAQVNLLLDPARSPDASLAERGLALRQVLALM
                     IAQRRPSYYLAQGRNDLADITDSHIRLLAGGGLIDSALRDAALRQQLTYRDWAQEPNL
                     QPVDSNKGISVSRARLSAMLNMPLYDLDRLDLSATSTLNNELQQAASQYLQQLADPEF
                     AGQIGLFGERLLSPEKTADVRYSFTLFERTSNGSVVRVQTDSTDQPFDINEGSKLELG
                     STAKLRVLATYLEIIAELHQRHAGQSASELRAVNAQDNLTRWAIGYLQSTPDASLTAM
                     LDAALERKYSASPAERFFTGGGIHTFNNFRREDNQRIASMREALRESLNLPFIRLMRD
                     LVRYSTYQGQNNSAELLKDDKDPRRQAYLQDFADREGTTFLLRFWRKYQGQPEQQRLD
                     TFLNGLRHTSVRLAAVHRYLMPHADEASFAAFLRKYLPQEKLSDKRIAELYKNYGPGA
                     YSLPDQGYIARVHPLELWLLGYLIEQPQATFSNAVAASQDERQEVYGWLFRSRHKSAR
                     DSRIRIMLEVEAFLDIHRRWQNLGYPFAHLVPSLATAIGSSGDRPAALAELVGIIQND
                     GIRQPTARIDSLHFAADTPYDTLVVREHTEGKRVMASEVAAALRSALADVVEAGTARR
                     LLGSFTQADGQPLKVGGKTGTGDNRIETVGAGGRVLSSRAMNRTATFVFYLGPRHYGT
                     LTAFVPGRAAESFRFTSALPVQVLKGMAPILLPYLEPDTHSQCLPPTPAVQARTL"
     gene            4264..4950
                     /locus_tag="VFF22_11520"
     CDS             4264..4950
                     /locus_tag="VFF22_11520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX17479.1"
                     /translation="MQRDHREQLNLSWQANADAWTAAVREQRIESRRLVTDAAIVQAI
                     LALAPKRVLDLGCGEGWLCRGLVEHGIEAVGVDASAPLIAVAQQAAAGTSQYRVCGYA
                     ELESQAELLGRFDVLVCNFALLEEPLAPTLNALHGLLAEDGRLLIQTLHPWRACNDAG
                     YRDGWRVETFAGFGEGFALPMPWFFRTLESWLGLFSETGWRLQWLQEPLHPESEQPVS
                     LLMLLSSERN"
     gene            complement(4969..5298)
                     /locus_tag="VFF22_11525"
     CDS             complement(4969..5298)
                     /locus_tag="VFF22_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HZX17480.1"
                     /translation="MNISDIPFGITDWSQVERSEHAGETGMAYWRTQVFGSIRVRMVE
                     YSAGYLADHWCQKGHILLCLEGELHTELEDGRRFVMTAGMSYQVADQAEAHRSSTASG
                     ARLFIVD"
     gene            complement(5323..6063)
                     /locus_tag="VFF22_11530"
     CDS             complement(5323..6063)
                     /locus_tag="VFF22_11530"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZX17481.1"
                     /translation="MRTLYQFPISHYCEKTRWHLDHKGLDFQVDNLFPGLHRLKSKRL
                     ADIVTLPILKDGEQVIGDSTQIALYLEQRYPEHPLLPTDAAQRAEVLELEEQFDRLGV
                     HVRRWLYGQIKQWDSVMHAMLKVYRPLLGLRDLMKPVLINGVQRLYGVTPPRVAKSQA
                     ELLEGLALIESRIHGDPSRYLVGEQLTLADISAAALFAPLFTPAGTPWENIAGHDAKT
                     QAFLDELHAHPAGQWVMRRYAEDRKRRN"
     gene            complement(6188..9334)
                     /locus_tag="VFF22_11535"
     CDS             complement(6188..9334)
                     /locus_tag="VFF22_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZX17482.1"
                     /translation="MHGLIAAALDRSRTSLLLLLFLMLGGLAAYFAIPKESNPDVSIP
                     IIYVSVTLEGISPEDAERLLVRPLEQELRSLEGVKEMRSMASEGHASVTLEFDAGFNA
                     KLALADVREKVDTARSKLPEEAEEPTVNEVNVALFPVLSIGLSGPIAETELVYIARRL
                     KENVEGIAEVLSVEIGGDREDLLEIVVDPQVLDSYGIDYNELFNLVSRNNRLVAAGSL
                     DTGAGRMSMKVPGVIEDLEDVLSMPIKVVGNSVVTFGDVATIHRTFKDPTGYARINGQ
                     PAVVLEVSKRSGANIIDTIEQVKALMVKAQPLLPEGLKVSYIMDQSQQVQSMLSDLLN
                     NVMTAIVLVLILVVASMGMRSALLVGLTIPGAFLTGILLIWMLGFTLNIVVLFSLILV
                     AGMLVDGAIVVSELADRYLHQGQTPRQAWANAATRMAWPVIASTATTLVVFLPLLFWP
                     GVVGQFMKYLPATVIVCLLASLAMALVFLPVLGAVTGGQPLPQATQPGRGAVGYRRLL
                     GTLLKRPGLTLLGILAVIALIYTAYGRFNHGVEFFPEVEPENAQIWLRARGDLSVQEK
                     DALLQQVEKRLLGMSEVKALYARSLAQPDGQLGADVIGTLQFQFVEWHERRSAGEILT
                     DMSTRTADIPGIVLEFRKQEEGPSSGKPVKLQVSSLDPAKADQWVEQVRANMQRLGGF
                     KDIEDDRALPGIEWRVKVDRESAARFGADVLSVGNAVQMVTNGLKLATYRPEDATDEV
                     DIRVRLPANWRSLDQLGRLTLNTPAGQIPLSNFVSLQQAPKVGTLRRVDGNRTITLQA
                     DMAEGARLDERLQALREAMGEVPSDVQVKFAGEDADQREAAAFLGTAFAVAIFLMFII
                     LVTQFNSIYQSLLVLSAIVLSTAGVLMGLLVNGQSFGIVMVGMGLIALAGIVVNNNII
                     LIDTYNQLRRQGLEPREAALETGSLRLRPVLLTAVTTVLGLVPMVIGVNVDLLTPSLG
                     FGAPSTQWWTQLSSAIAGGLSFATVLTLLLTPCLLVLGSRFERRPPPLETFDDDLLDL
                     PEHLLASATGKTELQRTP"
     gene            complement(9336..10418)
                     /locus_tag="VFF22_11540"
     CDS             complement(9336..10418)
                     /locus_tag="VFF22_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017679103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZX17483.1"
                     /translation="MFSQLSKRPWLIAVAISLLLVLWLFSGSLFKAQETATADAPAEK
                     QGLTKVEVQWLEASPMQRQHVVQGQIEAWRRVELRSQISGTVTRLDQDKGNPVKQGQL
                     LLSLSTDNRPAQVAKGEADVRQRAADASAAERLRQRNLVSTNELIRLQSELAKARAEL
                     DLARIQLGNTQISAPFAGVYDQRMVELGDFVQPGQSLLTLVDISQLKVSAQIAQQEVT
                     QLTLGQGVRVELLDGRELQGELHFIAAAADPGSRSFRIEVKVANPQALRLAGASATLH
                     IQTGEALAHRLSPALLSLDKAGRHGVKWVNEQQQVQFTPVQLISVDNQGAWVSGLPNK
                     VALITLGQGFVEPGQQVISQLAKGGS"
     gene            complement(10424..11080)
                     /locus_tag="VFF22_11545"
     CDS             complement(10424..11080)
                     /locus_tag="VFF22_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX17484.1"
                     /translation="MRYQDQLFEQRENALLDAARQLFAEQPWDRVTIAEVAIAAGIGK
                     GTVYKHFPSKEALYARLVLDLSRENLLELRQLHAASPAQDAMRRVIHRAFEQMLANPI
                     QAQLCLHCDRPEFQERLETPYRQQFLDMEREFQLFFSDMLGVALNNQALSQADCQNLL
                     WGVEACVNGVMARIASGGFAHWAEPIALDDYFARVTEFIIAGLRTQAAELLAHPHRHE
                     "
     gene            complement(11189..11953)
                     /locus_tag="VFF22_11550"
     CDS             complement(11189..11953)
                     /locus_tag="VFF22_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX17485.1"
                     /translation="MRRLLTALLLCTGLYQPAHSQEQIRIAIGEWPPFIGEALPHYGV
                     VPQLLNEAFATQGMSVEYGFFPWKRAYKEVKQGRWQASAIWGRTPEREADCLFSAVVY
                     SDEVVLFYRRDTPITWDGSLAGAEQLNGLRIGIPLGSAKMPVLEEAEQRGWVEYEASG
                     DELINLRKLAAGRIDAVDIVKGSGSHVLSQLSAKERARLTTTQTYETWDYHLIFSRQL
                     PESERLQQLFDQGLRELKNSGRYAQIWQQFNAPTAP"
     gene            complement(11988..12908)
                     /gene="metR"
                     /locus_tag="VFF22_11555"
     CDS             complement(11988..12908)
                     /gene="metR"
                     /locus_tag="VFF22_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MetR"
                     /protein_id="HZX17486.1"
                     /translation="MLELRHLKTLHALRESESLVDAAERLHLTQSALSHQFKELEERL
                     GMPLFVRKTKPVRFTSAGLRLLQLTDTVLPLLRGAERDLARLAGGTAGRLHMAIECHS
                     CFQWLMPTIDQFRDAWPEVELDLASGFSFAPLPALARGDLDLVVTSDPLELAGITYVP
                     LFTYEAMLAVANQHPLANKPCIKPQDLATETLITYPVERDRLDIFTRFLEPADVEPAQ
                     VRTSELTVMMMQLVASGRGVCGLPNWALHEYSSRGYVTAKRLGDKGLFATLYAGIRTD
                     MLDAPFMRDFLLTAKDTSFANLEGVSVARG"
     gene            13129..>14772
                     /gene="metE"
                     /locus_tag="VFF22_11560"
     CDS             13129..>14772
                     /gene="metE"
                     /locus_tag="VFF22_11560"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HZX17487.1"
                     /translation="MALSHSLGFPRIGRDRELKKALEAYWKGELDEAELQAVGLELRA
                     AHWQLQKDAGLDLLPVGDFAWYDQVLTHSLTFGVIPERFRPADGKPTLDTLFGMARGA
                     VGNKSSHNACCGAHAQEMTKWFDTNYHYLVPEFSADQQFQLSWEQLFDEVEDARALGH
                     KVKPVLIGPLTYLWLGKAKGSDFDKLDLLERLLPVYGEILQRLASQGVEWVQIDEPIL
                     VLDLPQAWKNAFERAYNLLQREPGKKLIATYFGGLEDNLGLAASLPVDGLHIDLVRAP
                     EQFPTILDRLPAYKVLSLGVVNGRNVWRTDLQPVLQILQQAQERLGERLWVAPSCSLL
                     HSPVDLNRENKLDGELKSWLAFAVQKCEEVALLTTALNDPHNVQVQTALAESRAVQAS
                     RAQSPRIHKPVVQARLAAISAADSQRQSPFATRIEQQRSRLQLPSFPTTTIGSFPQTA
                     EIRLARQSFKAGKLSLGDYTEAMHSEIRHAVEVQENLGLDVLVHGEAERNDMVEYFAE
                     QLDGYAFTRFGWVQSYGSRCVKPAVIYGDLSRPKAMTVAW"
BASE COUNT         2685 a         4320 c         4855 g         2912 t
ORIGIN      
        1 ggatttcgag cagttccgga ctttcctgga aaaagcctgc ggcatcctgc ttggcaccaa
       61 caagcagtat ctggtttcca gtcggctcaa caagttgatg gagcagaaca gcatcaagac
      121 cctgggtgag ctggtgcagc gtatgcagag tcagccacgc agcggtctgc gtgagcaggt
      181 ggttgatgcc atgaccacca acgaaactct gtggtttcgc gatacttatc cgttcgaggt
      241 gctgaagaac cgcgtgctgc cggagatgat caaggccagc ccgggtcagc gtctgcgcat
      301 ctggtcggcg gcttgctcct ccggtcagga accttattcg ttgtcgatga ctatcgatga
      361 gttcgagaag accaatatcg gtcagctcaa gggcggcttg cagattgtag ccaccgacct
      421 ttcaccgacc atgttgatca actgcaaatc cggtgagtac gacagcctgg cgatggggcg
      481 cgggctttcc caggagcgtc tgcagcgcta tttcgacccc aaggggccgg gtcgttgggt
      541 ggtcaaaccg gcgattcgca gccgcatcga gttccgtccg ctgaacctgc tggacagtta
      601 cgccagcctg ggcaagtttg atgtggtgtt ctgccgtaac gtgttgatct acttctcggc
      661 cgaggtgaag aaggacatcc tcacgcgcat tcatgccatg ttgaaacctg gcggttatct
      721 gttcctgggg gcttctgaag cgctcaatgg cctgcctaac cactaccaga tggtgcagtg
      781 cagcccagga atcatctata aggcgaagta ataatcgcaa ggctcaaaaa agcgggaggc
      841 cattggcctc ccgtttttat tgtgggcagt tttttcgacc ctacaaggtt ctggcctgaa
      901 ccgcaggcgt aggcggcaga cattggctgt gggtatccgg ctcaaggtag ggcagcagaa
      961 tcggcgccat gcctttgagg acctgcaccg gcagcgctga ggtgaagcgg aaactctcgg
     1021 cagcgcggcc agggacgaag gcggtaaggg tgccgtagtg gcgtgggccg aggtagaaga
     1081 caaaggttgc ggtgcggttc atcgcccgcg agctgagtac ccggccaccg gcaccgacgg
     1141 tttcgatgcg gttatcgccg gtgccggttt tgccgccgac cttgagcggt tggccgtcgg
     1201 cctgggtaaa gctgcccagc aagcgccgcg ctgtgccggc ttcaaccaca tcggcgaggg
     1261 cgctgcgtag cgccgccgcg acttccgagg ccatcacccg cttgccctca gtatgctcgc
     1321 gtaccaccag ggtgtcgtag ggcgtatcag cggcaaaatg cagactgtcg atgcgtgcgg
     1381 tgggttgccg aataccgtcg ttctggataa tcccaaccag ctcggccagc gccgccgggc
     1441 gatcgccgga gctgccgatg gcggtggcca gcgacggcac cagatgggca aagggatagc
     1501 cgagattttg ccagcgccgg tgaatatcga ggaaagcttc cacctccagc atgatacgga
     1561 tacggctgtc gcgggcgctc ttgtggcggc tgcggaacag ccaaccgtag acttcctgac
     1621 gctcgtcctg gctggcggcc acggcattgc tgaaggtggc ttgtggctgt tcgatcaggt
     1681 agcccaaaag ccacagttca agcgggtgga cgcgggcgat atagccctgg tccggcaggc
     1741 tgtaggcacc tggaccgtag tttttataca gctcggcgat gcgcttatcg ctgagttttt
     1801 cctggggcag gtatttgcgc aggaaggcgg cgaagctggc ttcgtctgca tgcggcatca
     1861 ggtaacggtg cacggcggcg agccgcaccg aggtgtggcg caggccgttg agaaaggtgt
     1921 ccaggcgctg ttgctcgggc tggccctggt atttgcgcca gaagcgcagc aggaaggtgg
     1981 tgccttcgcg gtcggcgaag tcttgcaggt aggcctggcg acgcgggtct ttgtcgtcct
     2041 tgagcaattc tgcgctgttg ttctggccct ggtaggtgct gtagcgcacc aggtcgcgca
     2101 tcaagcggat aaacggcagg ttgagcgact cgcgcagggc ttcgcgcatg ctggcgatgc
     2161 gctggttatc ttcgcggcgg aagttgttga aggtgtgaat gccaccgccg gtgaagaagc
     2221 gctctgccgg gctggcggag tatttgcgtt ccaatgcggc atccagcatg gccgtcaggc
     2281 tggcgtcggg ggtgctttgc aggtagccga tggcccagcg ggtgaggttg tcctgggcgt
     2341 tgacggcgcg caattcactg gcgctttggc ccgcatggcg ctggtgcagt tcggcgatga
     2401 tttccagata ggtggccagc actctgagct tggccgtgga gcccagttcc agtttgctgc
     2461 cttcgttgat atcgaacggc tggtcggtgc tgtcggtctg cacgcgcacc acgctgccgt
     2521 tactggtgcg ttcgaacagg gtgaagctgt agcgcacgtc ggcggtcttt tccggggaca
     2581 gaaggcgctc gccaaacaag ccgatctgcc cggcaaattc cgggtcggcc aattgctgca
     2641 ggtactggct tgcggcttgc tgcagctcgt tgttcagcgt gctggtggcg ctcaggtcga
     2701 gacggtccag gtcatacagc ggcatattga gcatggctga cagccgcgcc cgtgagacgc
     2761 tgatgccctt gttgctgtct accggttgca gattaggctc ctgggcccag tcgcggtagg
     2821 tgagttgctg acgcagggcc gcatcgcgca gcgccgagtc aatcaggccg ccgccggcca
     2881 gcaggcggat atggctatcg gttatatcgg ccaggtcatt gcggccctgg gccaggtaat
     2941 aggaggggcg gcgctgggca atcatcagag ccagcacctg gcgtagggcc aggccgcgct
     3001 cggccaggct ggcatccggt gaacgtgcgg ggtcgagcag cagattgacc tgggcgaagt
     3061 ccgcgccaaa ccagatgcgc aggccatcgg ccaggccatg cacttcgcca tgcccggggg
     3121 cggcggagag cggcacgcta ttgaggtagt cacggacgat attttcccgt gcggccagtg
     3181 tctgcgggcc gctctggtag gcgcgcacgc tggcggaaag catctgccgc agtttctccg
     3241 tggccgacaa ggtaaggcca tccggggagt gacggtattt ctccaactgg gtggccaggg
     3301 tgctaccgcc ggccgattgg tcctgcatat ccagcagttt gccgacctgg gagatggccg
     3361 ccatgacgaa tcgtggccaa tccactgctg ggttggccag tggctgctcg gggtcgagca
     3421 gcttgcggtt ttcgataaac agcaggctgt cggctaccag cggcgggatg gcgttgaagc
     3481 ttgggtagcg ctgctgcggg tagcggaatt catagaaggg cgtaccgctg cagtcattga
     3541 tgcgcaggcc gctctggatc ttctccgggt agggtacaaa caggccgcgc tgggcatagt
     3601 cgagcagggc gggggagaac tgcgcctgtt gctgtacggt aaaaccacgt tggtcgagcc
     3661 gctccagcat caagggcagg taggtgtagc cctggcgctt atcgaaaggg ccttccttgg
     3721 ggtagatgat cgacgggcta gggcccggct tgacccgata gccgagggtt tgcgcgtaac
     3781 gactaagttc ctgggcctga aagcgcgagg tgcgcacttc ctcgacaagc aaaaatccgg
     3841 cggcgatgca cagcagtaga acaagcaacc agaaggtgac cttcagctta ctggtgcgct
     3901 tgctggggcg gggttggtcg ccggccggca gggtggtgat gtcgagtttc catggtgcgc
     3961 ccatattcat ttagcctgat gtactcatcc ataggtttga caggttgctg aataacatca
     4021 gcgggcttga acactgagtt cctctcagct ggccactgtt tgcagtttag ccgctggtgt
     4081 agtgggcagg ccgggatgtg cttggcagac agaggatttt tcgctggttt tatgcgcagt
     4141 tgcggtcatt aagtgatcag cggcaacaca gaatggctgc aacaggcgca ggattgcccc
     4201 tggctctgcg tgttctgcag attgctagaa ttgtgccgtg acagaacagg cgacgagtga
     4261 gccatgcaac gcgaccatcg ggagcaactc aacctcagtt ggcaggctaa tgccgatgcc
     4321 tggacagcgg ccgtgcgcga gcaacgcatc gaaagccgcc gactggtgac ggatgccgct
     4381 atcgttcagg cgatcctcgc cctggcgccc aagcgggtgc ttgatctggg ctgtggcgag
     4441 ggctggttgt gccgtggcct ggtcgagcat gggattgagg cggtcggcgt ggatgcctct
     4501 gcgccgctga ttgctgtggc gcagcaggcg gcggcaggca cttcgcagta ccgcgtatgc
     4561 ggttatgccg agctggaaag ccaggctgag ctgctggggc gtttcgatgt gctggtgtgc
     4621 aactttgccc tgctggaaga gccgctggcg ccaaccctga atgccctgca tggcttgctt
     4681 gcagaagacg gccggctgct gatccagacc cttcacccct ggcgcgcctg caatgacgcg
     4741 ggttatcgcg acggctggcg ggtggaaacc tttgccggct ttggtgaggg ctttgccctg
     4801 ccgatgccct ggtttttccg cacgctggaa tcctggctgg ggctgttcag cgaaaccggt
     4861 tggcgcctgc agtggttaca ggaaccgttg caccctgaga gcgagcagcc ggtgtcgctg
     4921 ttgatgctgc tgagctccga gcgcaactga tcaggcctga taaagccatc agtcgacgat
     4981 aaacagccgc gccccgctgg cggtagatga acggtgcgcc tcggcctgat cggcgacctg
     5041 ataactcatg cccgccgtca tcacaaaacg ccggccatct tccagctcgg tgtgcagctc
     5101 accttccagg cacagcagaa tatggccctt ctggcaccag tgatcggcca ggtagccggc
     5161 gctgtactcg accatgcgca cgcggatgct gccgaaaacc tgggtccgcc aataagccat
     5221 gccggtttcg ccagcatgtt cgctacgctc gacctggctc cagtcggtga taccaaaggg
     5281 aatatcgctg atgttcatgt gctcatcctt taatcgggtc aatcagttgc ggcgcttacg
     5341 gtcttcggca tagcggcgca tcacccactg gccggccggg tgggcgtgca gttcatcgag
     5401 aaaggcctgg gtttttgcat catgcccggc gatgttttcc cacggcgtgc cggctggcgt
     5461 gaacagcggg gcgaacagcg cggcggcgct gatatcggcc agagtcagtt gttcacccac
     5521 cagatagcgt gatggatcgc cgtggatgcg cgattcgatc agcgccaggc cttcaagcaa
     5581 ttcggcctgg gatttcgcca ctcgcggtgg ggttacgcca tacaggcgct gcacaccgtt
     5641 gatcagcacc ggcttcatca ggtcgcgcag gcccagcagc gggcggtaga ccttgagcat
     5701 ggcgtgcatc accgagtccc actgcttgat ctggccatac agccaacggc gtacgtgcac
     5761 gcccaggcgg tcgaactgtt cttccagctc cagtacctcg gcgcgttgcg cggcatcggt
     5821 cggcagcagt gggtgctctg gatagcgctg ttccagatag agggcgattt gcgtggagtc
     5881 gccgatcacc tgctcgccat ccttgaggat cggcagggtg acaatgtccg ccaggcgctt
     5941 gctcttcagt cgatgcaggc cggggaagag gttgtcgacc tgaaagtcga ggcccttgtg
     6001 atctaggtgc cagcgggttt tttcgcaata gtgggaaatt ggaaactgat aaagcgtgcg
     6061 catggtcggc cctcggcaat gaggcaccga gtgtacgggc tggcggcctg actacccagt
     6121 cgtaagccgc atcccaggct ttctgggggc gggagccccc aggccattga acgcctgggc
     6181 gtgcggttta cggtgtacgt tgcagttcgg tcttgccagt ggcactggcc agcagatgtt
     6241 ccggcaagtc gagcaggtcg tcgtcgaagg tctccagcgg tggcgggcga cgctcgaagc
     6301 gcgagccgag taccagcagg cagggggtca gcagcagggt cagcacggtg gcgaagctca
     6361 ggccgccggc aatcgcgctg gacagctggg tccaccattg ggttgacggc gcgccaaagc
     6421 ccaggcttgg ggtgagcagg tcgacgttga ccccgatcac cattggcacc agcccgagca
     6481 ccgtggtcac ggcagtcagc agcaccgggc gcaagcgcag gctaccggtt tccagcgccg
     6541 cttcacgcgg ctccaggccc tggcggcgca gctggttgta ggtgtcgatg aggatgatgt
     6601 tgttgttcac cacgataccc gccagggcaa tcaggcccat gccgaccatc acgatgccaa
     6661 acgattggcc gttgaccagc aggcccatca gcactcctgc ggtggacagc acgatggccg
     6721 agagcaccag cagcgactga tagatgctgt tgaactgcgt caccagaatg ataaacatca
     6781 ggaagatcgc caccgcaaag gcagtgccga ggaaggccgc agcttcgcgc tggtcggcat
     6841 cctcaccggc gaatttcacc tgtacatcgc taggcacctc gcccatggct tctcgcaggg
     6901 cttgcaggcg ttcgtccagg cgtgcgcctt cggccatgtc agcttgcagg gtgatggtac
     6961 ggttgccgtc gacccggcgc agagtgccga ccttgggcgc ctgttgcagc gagacgaagt
     7021 tgctcagtgg aatctgcccg gccggggtat tcagggtcag ccggccgagt tggtctagag
     7081 agcgccagtt ggctggcagg cgcacgcgga tatccacttc atcggtggcg tcttccgggc
     7141 gataggtggc cagtttcagg ccattggtaa ccatctgcac tgcgttgccc acgctcagca
     7201 cgtcggcgcc gaaacgcgcg gccgactcgc ggtcgacttt gacccgccat tcaataccgg
     7261 gcagggcgcg gtcatcctcg atgtccttga aaccgcccag gcgctgcata ttggcgcgca
     7321 cctgttcgac ccactggtcg gctttggccg ggtccagcga gctgacctgc aatttgaccg
     7381 gtttgccgct gctcgggcct tcctcctgct tgcggaactc caggacgatg ccggggatat
     7441 ccgcagtgcg cgtgctcatg tccgtaagaa tctcaccggc cgaacgccgt tcgtgccatt
     7501 cgacaaactg gaactgcagg gtgccgatca catccgcgcc tagctgaccg tccggctgcg
     7561 ccagggagcg ggcatacagc gccttgacct cactcatgcc gagcagacgt ttttccacct
     7621 gttgcagcag ggcgtctttt tcctgcaccg acaaatcgcc gcgggcgcgc agccagattt
     7681 gcgcgttttc cggttcgact tcggggaaga actccacgcc atggttgaag cggccatagg
     7741 cggtgtagat cagtgcaatc accgcgagta tgcccaacag agtcaggccg gggcgcttca
     7801 gcaaagtgcc gagcaagcgg cgataaccga cggcgccacg gcctggttgg gtggcttgcg
     7861 gcaacggttg gccaccggtg actgcaccga gcaccggcag aaacaccagg gccatggcca
     7921 gggaagcgag caggcagacg atcaccgtgg ccggcaggta tttcatgaac tggccaacca
     7981 cgccgggcca gaacagcagc ggcagaaaca ccaccagggt agtggcggtg gaggcgatca
     8041 ccggccaggc cattcgcgtg gcggcgttgg cccaagcttg gcgcggcgtc tgcccctggt
     8101 gcaggtagcg gtcggccagc tcagacacca cgatggcgcc gtccaccagc atgccggcaa
     8161 ccagaatcag gctgaacagc acgacgatat tcagggtaaa gccgagcatc cagatcagca
     8221 gaatgccggt gagaaaggcc cctggaatgg tcagtccaac taatagcgcc gagcgcatgc
     8281 ccatgctcgc cactaccaga atcagcacca gcacgatggc ggtcatcacg ttgttgagca
     8341 ggtcgctgag catgctctgc acctgctgcg actggtccat gatgtagctg accttgaggc
     8401 cttcgggcag caatggctgg gccttgacca tcagcgcttt gacctgctcg atggtgtcga
     8461 tgatgttcgc accgctgcgc ttggacacct ccagcaccac cgccggctga ccattgatcc
     8521 gcgcgtagcc ggtggggtct ttgaaggtgc ggtggatggt ggcgacgtcg ccgaaggtca
     8581 ccacgctgtt gccgaccacc ttgatcggca tgctcagcac gtcttcgagg tcttcgatca
     8641 ccccgggcac tttcatgctc atgcgcccgg cgccggtgtc caggctgccg gcggcgacca
     8701 ggcggttgtt gcggctgacc aggttgaaca gttcgttgta atcgatgccg tagctgtcga
     8761 gcacctgcgg gtcgacgacg atttccaaga ggtcttcgcg gtcgccgccg atttccaccg
     8821 agagcacctc ggcgatgcct tcgacgttct ctttcaggcg gcgagcgata tacaccagtt
     8881 cggtttcggc aatcggcccg gacaggccaa tcgacagcac cggaaacagc gctacgttga
     8941 cttcattgac cgtgggttcc tcggcttctt ccggcagctt gctgcgggcg gtatcgacct
     9001 tctcacgcac atcggcgagg gccagcttgg cgttgaagcc ggcatcgaac tccagggtga
     9061 ccgaggcatg gccttcgctg gccatggaac gcatttcctt gaccccttcg aggctgcgca
     9121 gctcctgctc cagcggcctt accagcaggc gttcggcgtc ttccgggctg atgccttcga
     9181 gggttaccga gacatagatg atcgggatgc tgacatccgg gttggactcc ttggggatgg
     9241 cgaaatacgc cgccaggccg ccgagcataa gaaacagcag caacaacagg ctggtgcggc
     9301 tgcgatccag cgccgcagcg atcaggccat gcatatcagc tacctccttt ggccagctgg
     9361 ctgatgacct gctgccctgg ttcgacaaag ccctggccga gggtgatcaa ggccactttg
     9421 ttgggcaggc cgctgaccca ggcgccctgg ttgtcgacgc tgatcagctg cactggggtg
     9481 aactgcacct gttgctgctc attgacccac ttcacgccat gccggccggc tttatccagg
     9541 ctcaggaggg caggcgacaa acgatgggcc agggcttcgc cggtctggat atgcagggtt
     9601 gcactggcac ccgccaggcg cagggcctgc gggttggcga ccttgacctc gatgcggaag
     9661 ctgcgtgagc cggggtcggc ggcggcggcg ataaagtgca gctcgccctg cagctcgcgg
     9721 ccatcgagca actcgacgcg cacgccttgg cccagggtca gttgagtgac ttcctgctgg
     9781 gcgatctgcg cgctgacctt gagctggctg atgtccacca gggtcagcag gctctgcccc
     9841 ggctggacga aatcgcccag ttcgaccatg cgctggtcat acaccccggc gaagggggcg
     9901 ctgatctgcg tgttgcccag ctgaatgcgc gccagatcga gttcggcgcg agccttggcc
     9961 agctcgcttt gcaggcggat cagctcgttg gtggaaacca ggttgcgttg acgtaggcgt
    10021 tcggcggcgc tggcatcggc ggcgcgctgg cgtacatcgg cctcaccctt ggccacctgg
    10081 gccgggcggt tgtccgtgga caggctgagc agcagctgac cttgtttgac cgggttgcct
    10141 ttgtcctgat ccaggcgcgt caccgtgccg ctgatctgcg agcgtagttc gactcggcgc
    10201 caggcttcga tttgcccctg caccacatgc tgacgctgca ttgggctggc ttccagccac
    10261 tgcacttcaa ccttggtcag gccctgtttc tctgccggcg catcggccgt ggcggtttcc
    10321 tgcgccttga acaacgagcc gctgaacagc cagagcacga gcagcagact gatggcgacg
    10381 gcgatcaacc aggggcgttt ggacaactga ctgaacatgg tggctactcg tggcgatgag
    10441 ggtgagcaag cagttcggcg gcctgggtac gcaggccggc aatgatgaat tcggtgacgc
    10501 gggcgaaata gtcatccagg gcaatcggct cggcccaatg ggcaaagccg ccggaggcga
    10561 tacgcgccat caccccgttg acgcaggctt cgacgcccca cagcaggttc tgacagtcgg
    10621 cctggctcag tgcctgattg ttcagggcga cgccgagcat atcgctgaag aacagctgga
    10681 attcgcgctc catatcgagg aactgctggc ggtacggggt ttccaggcgt tcctgaaact
    10741 ccgggcggtc acagtgcaaa cacagctgcg cctggatcgg gttggccagc atctgctcga
    10801 aggcgcggtg gatcacccgg cgcatggcgt cctgcgcggg gctggcggca tgcagctggc
    10861 gcagctccag caggttttcg cggctgaggt cgagtaccag gcgtgcataa agcgcttcct
    10921 tgctggggaa gtgcttgtat accgtgcctt tgccgatacc ggcggcaatc gccacttcgg
    10981 caatggtcac gcggtcccag ggctgttcgg caaacagctg gcgggcggcg tcgagaaggg
    11041 cgttctcgcg ttgttcgaag agttgatctt ggtagcgcat gggcatttat cagaggttgt
    11101 gaccggtggt catgctatga ctgttggtca cgactgggca agccggcagt tgtaacaggc
    11161 tttgccgcgg ctcgattgga ctgggctgtc agggggcggt gggcgcgttg aactgctgcc
    11221 agatctgcgc gtaacggccg ctgttcttga gttcacgcag gccctggtcg aacagttgct
    11281 gcaggcgttc gctctcgggc agctggcggg agaagatcag gtggtagtcc caggtttcat
    11341 aggtttgcgt ggtggtcagg cgtgcccgct ccttggcact gagctggctg agcacatggc
    11401 tgcccgagcc tttgacgatg tccactgcat cgatgcgccc ggcggccagc ttgcgcaggt
    11461 tgatcagctc atcaccgctg gcttcgtact ctacccagcc gcgctgttcg gcttcttcaa
    11521 gtaccggcat cttcgctgag ccgagtggaa taccgatgcg caggccattg agctgctcgg
    11581 ccccggccag gctgccgtcc caggtgatgg gggtgtcgcg gcggtagaac agcaccactt
    11641 catcgctata caccacagcg gaaaataggc agtcagcctc gcgctccggt gtgcggcccc
    11701 agatagccga ggcttgccag cggccttgct tgacctcttt gtaggcacgc ttccagggga
    11761 agaagccgta ttccacgctc attccctggg tggcgaaggc ttcgttgagc aactgcggca
    11821 ccacgccata gtgcggcagc gcctcaccaa taaagggcgg ccattcaccg atggcgatgc
    11881 ggatctgttc ctggctatgt gcgggctggt agaggcctgt gcagagcagc agagcggtga
    11941 gcaaacggcg catgcagtgc tcaatattca ggtggttggg ttcaagctta gccgcgtgcc
    12001 acgctgacgc cttccagatt ggcgaaggag gtgtccttgg cggtcagcag aaaatcgcgc
    12061 ataaacggtg catcgagcat atcagtgcgg atgccggcat acagggtggc gaacaggccc
    12121 ttgtcaccca ggcgtttggc ggtcacgtag ccgcgcgagc tgtattcgtg cagcgcccag
    12181 ttgggcaggc cgcagacccc acggccgctg gccactagct gcatcatcat caccgtcagc
    12241 tcggaggtgc gcacctgcgc tggttcgaca tctgccggct ctaggaaacg cgtgaagata
    12301 tccaggcgat cgcgttccac cgggtaggtg atcagggttt ccgtggccag gtcctgcggc
    12361 ttgatgcacg gcttgttcgc cagcggatgc tggttggcca ccgccagcat ggcttcatag
    12421 gtaaatagcg gcacataggt aataccggcc agctccaacg gatcgctggt taccaccagg
    12481 tccagatcac cgcgcgccag agccggcagc ggggcgaagg agaagcccga ggccaaatcc
    12541 agttccacct ccggccaggc atcgcggaac tgatcaatgg tcggcatcag ccactggaag
    12601 cagctgtggc attcgatggc catatgcagg cggccggcgg tgcctccggc caagcgtgcc
    12661 aggtcgcgtt cggcaccgcg caacagcggc aataccgtgt cggtcaattg cagcaaacgt
    12721 aaaccggcgc tggtgaaacg caccggtttg gtcttgcgca caaacaacgg catacccaaa
    12781 cgctcttcca actccttgaa ctgatgcgac agcgccgatt gggtcagatg caggcgctca
    12841 gcggcgtcca ccaggctttc gctttcacgc agggcatgca gggttttcag gtggcgcaat
    12901 tcgagcatgg cggggctcca gggtatcttg cagatgtgct gtgtgtagga gcgagcttgc
    12961 tcgcgatcag gttggcgaga gcatcgcgag caagctcgct cctacaaata tgtgggttag
    13021 cagaacatga gtaaaactat agatcaaggc gaacagattg agtttgtctc acttaggcag
    13081 ggtgccgaga atagccagca tcttattgat ctgctggagc gttctgacat ggccctgtcc
    13141 cattcccttg gttttccgcg tatcggtcgc gaccgtgaac tgaaaaaagc cctggaggct
    13201 tactggaaag gcgagctgga cgaggccgag ctgcaggcgg tgggtcttga actgcgcgcc
    13261 gcccactggc agctgcagaa ggacgccgga cttgacctgc tgccggtcgg cgactttgcc
    13321 tggtacgacc aggtgctcac ccactcgctg accttcggcg taatccccga gcgcttccgc
    13381 ccggccgacg gcaagccgac cctggacacc ctgttcggca tggcgcgcgg tgccgttgga
    13441 aacaagagca gtcacaacgc ctgttgcggt gctcatgccc aagagatgac caagtggttc
    13501 gacaccaact accactacct ggtccccgag ttcagcgccg atcagcagtt ccagctgagc
    13561 tgggaacaac tgttcgatga agtagaagac gcacgcgctc tgggccacaa ggtcaaaccg
    13621 gtgctgatcg gcccgctgac ctatctgtgg ttgggcaaag ccaagggcag cgatttcgat
    13681 aagctcgatt tgctcgagcg gctgctcccg gtctacggcg aaatcctcca gcgtctggct
    13741 tcccagggtg tggaatgggt gcagatcgat gagccgattc tggtgctgga tttgccgcaa
    13801 gcctggaaga atgccttcga gcgtgcctac aacctgctgc agcgcgagcc aggtaagaag
    13861 ttgatcgcca cctatttcgg cggtctggaa gacaacctcg gtctggccgc tagcctgccg
    13921 gtggatggcc tgcatatcga cctggtgcgt gcgcccgagc agtttccgac catcctcgac
    13981 cgtttgccgg cgtacaaggt gttgtccctg ggtgtggtta acggccgcaa cgtctggcgc
    14041 actgatctgc agccggttct gcagattctg cagcaggctc aggagcgcct gggcgagcgc
    14101 ctgtgggtgg cgccaagctg ctcgctgctg cacagcccgg tcgacctgaa ccgtgaaaac
    14161 aagctggatg gcgagctgaa aagctggctg gcttttgccg tgcagaagtg cgaggaagtc
    14221 gcgctgctga cgacggcctt gaatgacccg cacaacgtcc aggtgcagac cgcgctggcg
    14281 gaaagccgcg ccgtgcaggc cagtcgcgcg caatcgccac gaatccacaa gccggtagtg
    14341 caggcgcgtc tggcggccat tagcgccgcc gacagccagc gccagtcgcc gtttgccacg
    14401 cgcatcgaac agcaacgttc acgcctgcag ttgccgtcgt tcccgacaac gaccatcggt
    14461 tcgttcccgc agaccgctga gatccgcctg gcgcgccagt cgttcaaggc cggcaaactg
    14521 tcgctgggtg actacaccga ggccatgcac agcgagattc gccacgccgt cgaggtgcag
    14581 gaaaatctcg gtctggatgt gctggtgcac ggtgaggccg agcgcaacga tatggtcgag
    14641 tacttcgccg agcagctcga cggctatgcc tttacccgtt tcggttgggt gcagagctac
    14701 ggttcgcgtt gcgtaaaacc ggcagtgatc tacggcgacc tgagtcggcc caaggccatg
    14761 actgtggcct gg
//
LOCUS       DASRUM010000178         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_926261, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000178 DASRUM010000000
VERSION     DASRUM010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926261"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..814
                     /gene="map"
                     /locus_tag="VFF22_11565"
     CDS             32..814
                     /gene="map"
                     /locus_tag="VFF22_11565"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009686955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZX17488.1"
                     /translation="MTVTIKTPDEIEKMRVAGRLAAEVLEMIGEHVKPGITTEALDRI
                     CHDYIVNVQQAIPAPLNYKGFPKSICTSVNHVVCHGIPNDKPLKEGDVINIDITVIKD
                     GYHGDTSKMFMVGKVPEWAERLAKITQECMYKGIELVKPGTRLGDIGEVIQKHAEKNG
                     FSVVREYCGHGIGAVFHEEPQVLHYGRAGTGMELKEGMTFTIEPMINQGRPETRTLGD
                     GWTAITKDRKLSAQWEHTILVTADGYEIFTLRNDDTIARTSA"
     gene            857..>2338
                     /gene="glnD"
                     /locus_tag="VFF22_11570"
     CDS             857..>2338
                     /gene="glnD"
                     /locus_tag="VFF22_11570"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HZX17489.1"
                     /translation="MPQVDPELFDRGQFQAELALKASPIAAFKKAIRHAREVLDGRFT
                     SGRDVRRLVEDRAWFVDQILREAWDRLSWSEDADIALLAVGGYGRGELHPFSDIDLLI
                     LLDNADHETFREPIERFLTLLWDIGLEVGQSVRSVDECAEEARADLTVITNLMESSTI
                     AGPERLRQRMQLVTSTEQMWPSKQFFLAKHKEQRARHGKYNDTEYNLEPNVKGSPGGL
                     RDIQTILWVARRHFGTLNLHAMVGQGFLLESEYALLASSQEFLWKVRYALHMLAGRAE
                     DRLLFDHQSRVAALLGYEDSDSKRAIERFMQKYYRVVMAIAELSDLIVQHFEEQILNA
                     GEIGQATPLNSRFLLRDGYIEVAHPNVFKRTPFAIMEIFVLMAQHPDIKGVCANSIRL
                     LREHRHLIDDDFRRDIRNTSLFVELFKSPQGIHRNLRRMNRYGILGLYLPEFGHIVGQ
                     MQHDLFHIYTVDAHTLNLIKHLRKFKWTELAEKFPLASKLIDKL"
BASE COUNT          528 a          763 c          646 g          401 t
ORIGIN      
        1 cgccctttct gttcgcgttc gagagccgca catgaccgtc accatcaaaa cgcccgacga
       61 aatcgagaaa atgcgcgtag ccggtcgcct ggctgccgaa gttctggaaa tgattggcga
      121 gcacgtcaag cccggcatca ccaccgaagc gctcgaccgt atttgccatg actatattgt
      181 caacgtgcaa caggccatcc ccgctccgct caattacaaa ggcttcccca agtcgatctg
      241 cacttcggtc aaccacgtgg tctgccacgg catcccgaat gacaagccgc taaaagaagg
      301 cgacgtgatc aacatcgaca tcaccgtgat caaagatggc taccacggcg ataccagcaa
      361 gatgtttatg gtcggcaaag tacccgagtg ggccgagcgc ctggccaaga tcacccagga
      421 atgcatgtat aaagggattg aactggtcaa gccaggcacg cgcctgggcg acatcggtga
      481 agtaatccag aagcatgccg aaaagaacgg tttctccgtg gtgcgcgaat actgcggcca
      541 cggcattggc gcggtgttcc atgaagagcc gcaagtgctg cactacggcc gcgccggcac
      601 cggcatggaa ctgaaggaag gcatgacctt caccatcgag ccgatgatca accagggccg
      661 cccggaaacc cgcaccctag gtgacggctg gaccgcgatc accaaagacc gcaagctctc
      721 cgcccagtgg gagcacacca ttctggtgac tgcagacggc tacgagatct tcaccttgcg
      781 caacgacgac accatcgccc gcacatcggc ctgagcctct cgctgagtct gctttataga
      841 tagaaggaag gccgcaatgc cgcaggtaga tcccgagctg ttcgaccgcg gccagttcca
      901 ggcggaactg gccctcaagg ccagccccat cgcggcattc aagaaggcca tccgccacgc
      961 ccgtgaagta ctcgacgggc gcttcaccag tggccgcgac gtacgccgac tggtcgagga
     1021 ccgagcctgg tttgtcgacc agatcctacg cgaagcctgg gaccgcctgt cctggagcga
     1081 agacgccgat atcgccctgc tggctgttgg cggctatggc cgcggcgagc tgcatccctt
     1141 ctccgacatc gacctgctga tcctgctcga caacgccgac cacgaaacct tccgcgagcc
     1201 catcgaacgc ttcctcaccc tgctgtggga catcggcctg gaagtcgggc aaagcgtgcg
     1261 cagtgtggat gagtgcgccg aggaagcccg cgccgacctg acggtgatca ccaacctgat
     1321 ggaaagcagc accatcgccg gccccgaacg cctgcgtcag cgtatgcagt tggttaccag
     1381 caccgagcag atgtggccga gcaagcagtt cttcctggcc aagcacaaag aacagcgcgc
     1441 ccgtcacggc aaatacaacg acaccgaata caacctggag cccaacgtca aaggctcgcc
     1501 cggtggcctg cgcgacattc agaccatcct ctgggtggcg cgccggcatt tcggcacgct
     1561 gaacctgcac gccatggtcg gccagggctt tttgctggaa agcgaatacg ccctgctcgc
     1621 ctccagccag gaattcctct ggaaagtacg ctacgccctg cacatgcttg ccggccgcgc
     1681 cgaagatcgc ctgctgttcg accaccagag ccgggttgcc gccctgctcg gctatgaaga
     1741 cagcgacagc aagcgcgcca ttgagcgctt tatgcagaag tactaccgcg tggtgatggc
     1801 gattgccgag ctcagcgacc tgatcgtcca gcacttcgaa gagcagatcc tcaacgctgg
     1861 cgaaatcggc caggccacac cgctcaacag ccgcttcctg ctgcgtgatg gctatatcga
     1921 agtcgcgcac cccaacgtgt tcaagcgcac gccgttcgcc atcatggaaa ttttcgtact
     1981 gatggcgcaa cacccggata tcaaaggcgt gtgcgccaac agcattcgcc tgctgcgcga
     2041 acaccgccac ctgatcgacg atgatttccg tcgcgatatc cgcaacacca gcctgttcgt
     2101 cgagctgttc aaaagcccgc agggcattca ccgcaacctg cggcggatga accgttacgg
     2161 catcctcggc ctgtacctgc cggaattcgg ccatatcgtc gggcagatgc agcacgacct
     2221 gttccacatc tatacggtcg atgcgcacac cctgaacctg atcaagcacc tgcgtaagtt
     2281 caagtggacc gagctggcgg aaaaattccc cctggccagc aaactgatcg acaagctg
//
LOCUS       DASRUM010000179         5157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1613229, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000179 DASRUM010000000
VERSION     DASRUM010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613229"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VFF22_11575"
     CDS             <1..622
                     /locus_tag="VFF22_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HZX17490.1"
                     /translation="WALAAYLIDVSGAQRELLEGVTALFASVMVLWLGVWMHDRRHAA
                     AWQDYIKSSLVGGGGRFGFAVLAFFSVYRELFEVILFYETLWLQAGPQGHGMVLAGAA
                     AALVLLLGLAWVILRGSRKLPLATFFGVNAVLLCVLSVVFAGHGVAALQEAGVLGTRP
                     VAFFEFDWLGIHDDAYSLAAQAAALVAITLLYGRSWLGERRRLSQA"
     gene            686..1138
                     /locus_tag="VFF22_11580"
     CDS             686..1138
                     /locus_tag="VFF22_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HZX17491.1"
                     /translation="MRVWIDADACPKAAKDQVVKFALKRRFEVLLVAGQAQIKPAYSC
                     VRLIVVPSGPDAADDYLVEHALPGELVICSDVPLADRLVKKGVAALDPRGREFDERNM
                     GDKLAVRNLFTELREQGQVGGGQPPYGERDKQAFANSLDRILTRLQRA"
     gene            complement(1142..1612)
                     /locus_tag="VFF22_11585"
     CDS             complement(1142..1612)
                     /locus_tag="VFF22_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZX17492.1"
                     /translation="MSNPPFELTAELQQAVSSFFQRIPFNQVLGIELDELSAQRVTMH
                     LPMKPELIGNFVHGILHGGVISSLLDVAGGAMALIGAFDKHQHLSSAERMTRLSKLGT
                     IDLRIDYLRPGRGQRFTATAVLLRTGNKVAVVRSELHSDDGTLVAVGTGTYLCG"
     gene            complement(1750..2412)
                     /gene="elbB"
                     /locus_tag="VFF22_11590"
     CDS             complement(1750..2412)
                     /gene="elbB"
                     /locus_tag="VFF22_11590"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010656881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HZX17493.1"
                     /translation="MNKKVAVILSGCGVYDGAEIHESVITLLRLDQRGAQVQCFAPNV
                     PQLHVVDHYSGDEMDDTRNVLVESARIARGKIKDVKELHVGDFDALIMPGGFGVAKNL
                     SDFAISGANCTVQPDVLSATQAFVAAGKPVGLMCIAPALAAKIFGPGVICTIGQDHET
                     AAAVVQMGAEHHECEVSEIVEDVERKLVTTPAYMLAQSISEAAAGINKLVDRVLELSH
                     PA"
     gene            2608..3843
                     /locus_tag="VFF22_11595"
     CDS             2608..3843
                     /locus_tag="VFF22_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HZX17494.1"
                     /translation="MNYILYAVPFFFALIALELLLDRWRGVNTYRFSDAVNSLSAGVL
                     STTVGLLTKAVGLLTYTLAWQKLALFDLSASSLWVWLFAFVFYDFCYYWNHRLGHERN
                     VLWAAHSVHHQSEDYNLSTALRQTSTGFIFGWIFYLPMAVAGVPPLVFLTVAALNLLY
                     QFWVHTRHIPKLGWFEWAFITPSNHRVHHAQNVVYMDRNYGGVFIIWDRLFGTFQEEL
                     DEAPVVFGVTTPLASWNPLWANAQFYVALWRDAVRAESWWDKLRIWFMRTGWRPADVA
                     AKYPQSKPDLSQFVKFDVPLSLAQKFYAGVQFSLYVAGGTLLLGMGGELSLAQTLLGV
                     TWMALGLYIIGNWLENRPNAKRLEVLRLLLNLPLAWLGLQLGLLPAQPLIWALLLGYS
                     LLSLGALFWTPRGQDAVPA"
     gene            4089..5102
                     /gene="hemB"
                     /locus_tag="VFF22_11600"
     CDS             4089..5102
                     /gene="hemB"
                     /locus_tag="VFF22_11600"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HZX17495.1"
                     /translation="MSFTPANRLFPATRLRRNRRDDFSRRLVRENRLSVDDLILPVFV
                     LDGENRREAIASMPGVERLSIDLLLKEAEHWVALGIPALALFPVTPLEKKSLLAEEAW
                     NPDGIAQRAIRALRAKFPELGVISDVALDPFTTHGQDGILDESGYVQNDITVDALVKQ
                     ALSHAAAGAQVVAPSDMMDGRVQAIREALELADHNNVRIMAYSAKYASAYYGPFRDAV
                     GSAANLGKANKASYQMDPANGDEALHEVAADLAEGADMVMVKPGMPYLDILWRVKAEF
                     KVPTFVYQVSGEYAMHMAAIENGWLGEAVILESLTAFKRAGADGILTYFAVRAAELLK
                     QGQ"
BASE COUNT          847 a         1519 c         1683 g         1108 t
ORIGIN      
        1 ctgggcgttg gcggcgtatt tgattgatgt cagcggcgcg cagcgtgaac tgctcgaagg
       61 cgtgactgcg ctgtttgcca gcgtgatggt gctctggctc ggggtgtgga tgcatgatcg
      121 ccggcatgcg gcggcctggc aggattacat caaaagcagc ctggtcggcg gtggtggccg
      181 tttcggtttt gccgtgctgg cgttcttctc cgtgtaccgc gagttgttcg aagtcatcct
      241 cttctatgaa accctctggc tgcaggccgg cccgcaaggc cacggcatgg tcctggccgg
      301 cgcggcggcg gctctggttc tgctgctggg cttggcctgg gtgattctgc gtggttcacg
      361 caaactgccg ctggcgacct tcttcggcgt caatgccgtg ctgctctgcg tgctctcggt
      421 ggtgtttgcc ggccatggcg tagcggccct gcaggaagcc ggggtgctcg gtacgcggcc
      481 ggtggcattc ttcgagttcg actggctggg tattcacgat gatgcctaca gtcttgcggc
      541 ccaggccgcg gcgctggtcg ccattactct gctttacggg cgtagctggt tgggcgagcg
      601 tcgccgtctg tctcaggcat gaagcgcagc tgctgccggc atggcttaac atgccggctt
      661 tgctgcctct ggagaatgct gggtgatgcg agtttggatt gatgccgatg cctgccccaa
      721 ggcggccaag gatcaggtgg tgaagttcgc tttgaagcgg cgcttcgagg tgttgctggt
      781 agcggggcag gcgcaaatca agccggcgta cagctgcgtg cggctgatcg tggtgcccag
      841 cggtccggat gccgccgatg actacctggt ggagcatgcc ctgccgggtg agctggtgat
      901 ctgcagcgat gtgccgctgg ccgaccgtct ggtgaagaag ggcgtcgcgg cccttgatcc
      961 gcgcggccgc gagtttgatg agcgcaatat gggcgacaag ctggcggtgc gtaacctatt
     1021 taccgagctg cgtgagcagg gccaggttgg tggtggtcaa ccgccttatg gcgagcgcga
     1081 caagcaggcg tttgccaatt cactggatcg tatcctgacc cgcttgcagc gtgcttaacc
     1141 attagccgca caggtaggta ccggtgccga ccgccaccag ggtaccgtcg tcgctgtgca
     1201 gttcgctgcg cactaccgcc accttgttgc cggtgcgcag cagcaccgca gtggcggtaa
     1261 agcgctggcc gcggccgggg cgcaggtagt cgatgcgcag gtcgatggtg ccgagcttcg
     1321 acagccgcgt catgcgttcg gcgctggata aatgctggtg cttgtcgaag gcaccgatca
     1381 gcgccatggc gccgccagcc acatccagca gcgaggaaat cacaccgcca tgcagaatgc
     1441 cgtggacgaa gttaccgatc agctcgggct tcatcggcag gtgcatggtc acgcgctggg
     1501 cgctcagctc atcgagctca atgccgagca cctggttgaa cgggatgcgc tggaagaagc
     1561 tgctgaccgc ttgttgcagc tcggcggtga gctcgaaagg tgggttgctc atgggagtcg
     1621 tgcctgggcg gatggatgat caacctgccg cactaaggcc gcggtgacca gtggcgcggc
     1681 cggagtaccg cgcgcattgg cgtgattggc gtgctatggg cctgatctgc ggctgatcag
     1741 accgcattct caggccgggt ggctgagctc cagcactcgg tcgaccagct tgttgatgcc
     1801 tgcggcggct tcactgatcg actgggcgag catataagcc ggggtggtta ccagcttgcg
     1861 ttcgacgtct tcgacaatct cgctgacttc gcactcatgg tgttctgcgc ccatctgcac
     1921 cacggcggct gcggtttcat ggtcttggcc gatggtgcag atcacgcctg ggccgaagat
     1981 cttcgccgct agcgccgggg cgatacacat cagcccgacc ggcttaccgg ctgctacgaa
     2041 ggcctgggtg gcgctcagca catccggctg caccgtgcag ttagcgccgc tgatggcgaa
     2101 gtcggagagg tttttcgcta cgccaaaacc gccgggcatg atcagcgcat cgaagtcgcc
     2161 gacatgcagc tccttgacgt ccttgatctt gccgcgggca atgcgcgccg actccaccag
     2221 tacgttgcgc gtgtcgtcca tctcgtcgcc actgtagtga tcgaccacgt gcagctgcgg
     2281 tacgttgggg gcaaagcact gcacctgcgc gccacgctga tcgaggcgca gcagggtgat
     2341 cacgctttca tggatttccg cgccgtcata gacgccacag ccggaaagaa tcaccgcgac
     2401 tttcttgttc atcaagttct cctgaattgc cagccgtaac ggattgagtc gtgacctagc
     2461 gtcgtttgag cactgttgtc gtcatatgcc acccgggttg gcatggcgcg ccgcgtggtg
     2521 gatgcggcgc tgggctagga tttattgagc aatcctagcc gttagcctcg tgcagcgata
     2581 tggcgattct atgcaaccgg tgaaaccatg aattacatcc tctatgccgt accgttcttc
     2641 tttgccttga tcgccctgga actgctgctc gaccgctggc gcggggtcaa cacctaccgc
     2701 ttctccgatg cggtcaacag cctcagcgcc ggggtgctgt cgaccaccgt gggcctgctg
     2761 accaaggccg tcggtctgct tacttacacc ctggcctggc aaaagctggc gctgtttgat
     2821 ctgtcggcga gcagcctgtg ggtctggctg ttcgccttcg tcttctacga cttctgctac
     2881 tactggaacc accgcctggg ccacgagcgc aatgtgctct gggcggcgca ctcggtgcac
     2941 catcagagtg aggactacaa cctttccacc gcgctgcgac agaccagcac cgggtttatc
     3001 ttcggctgga tcttttacct gccgatggct gtggccggtg tgccgccgct ggtatttctg
     3061 accgtggcgg cgctgaacct gctgtatcag ttctgggtgc acacccggca cattcccaaa
     3121 ctgggctggt ttgagtgggc gtttatcacg ccgtccaacc accgcgtgca ccatgcgcag
     3181 aatgttgtgt atatggatcg caattacggc ggagtgttca ttatttggga tcgcttgttc
     3241 ggcacctttc aggaggagct ggacgaggcc ccggtggtgt ttggcgtaac cacgccgctg
     3301 gccagctgga acccgctgtg ggccaatgcg cagttctatg tcgcgttgtg gcgtgacgca
     3361 gtgcgcgcag agtcctggtg ggacaagctg cgcatttggt ttatgcgcac tggctggcgg
     3421 ccggccgatg ttgcggcgaa gtacccgcag agcaagccgg acctcagcca gttcgtcaaa
     3481 ttcgatgtgc cgctgagcct ggcgcagaaa ttctacgccg gcgtgcagtt cagcctctat
     3541 gtggcaggcg gcaccttgct gctgggtatg ggcggcgagt tgagcctggc gcagacgctg
     3601 ttgggcgtga cctggatggc gctgggcctg tacatcatcg gcaactggct ggagaaccgg
     3661 cccaacgcca aacgcctgga agtgctgcgc ctgctgctca acctgccgct ggcctggctg
     3721 gggttgcagt tgggcctgtt gccggcccag ccgctgattt gggcgctgct gctgggttac
     3781 agtctgctca gcctgggtgc gctgttctgg accccgcgcg ggcaggatgc cgtacccgcc
     3841 taaaggttat tcatagcagg acccaacaac ggtggacagg cccaggcctg ctccaccgtt
     3901 gtgctttccg gtccgagaaa acggctttgg tgcatacgca tgcaacatat cttcgccata
     3961 gttgcggcgc tatgcttgtt gggcttgcgg caagcggtcg cagcgctgtc gtgcgttgtc
     4021 atactttggt cataatgtcc gcttataact gtcacacttg cccgtcgcta cgggcttcgg
     4081 agcctgccgt gagctttacc cccgccaatc gcctgtttcc cgccactcgc ctgcgccgca
     4141 accgccgtga tgatttttcc cggcgtctgg tgcgggaaaa ccgcctgagt gtcgacgacc
     4201 tgattctgcc ggtgttcgtc ctcgatggtg aaaaccgccg cgaagcgatt gcttcgatgc
     4261 ccggcgtcga gcgtctgtct atcgacctgc tgctcaaaga agccgagcac tgggtggccc
     4321 tgggtatccc ggcgctggcg ctgttcccgg tgacgccact ggagaagaaa tccctgctgg
     4381 ctgaagaagc ctggaacccg gatggcattg cccagcgcgc cattcgtgcc ctgcgcgcca
     4441 agttccccga gctgggggtg atcagcgatg tggcgctgga cccgtttacc acccacggtc
     4501 aggacggcat cctcgatgag tccggctatg tgcagaacga catcaccgtc gatgcgctgg
     4561 tcaagcaggc gctgtcgcat gccgccgccg gtgcccaggt ggtcgcgccc tcggacatga
     4621 tggacggccg cgtacaggcc atccgcgagg cgctggagct ggccgatcac aacaatgtgc
     4681 gcatcatggc ctactcggcc aagtacgcca gcgcctacta cggcccgttc cgcgatgcgg
     4741 tgggttcggc ggcgaatctg ggcaaggcca acaaggccag ctaccagatg gacccggcca
     4801 atggcgacga agcgctgcac gaagtggccg ccgatctggc cgaaggtgca gacatggtca
     4861 tggtcaagcc ggggatgccc tatctggaca tcctctggcg ggtcaaagcg gaattcaagg
     4921 tgccgacctt tgtttaccag gtcagcggtg agtacgccat gcatatggcg gctattgaaa
     4981 atggttggtt gggcgaggcg gtgattcttg aatcattgac tgccttcaaa cgtgcgggtg
     5041 ccgatggcat cctgacttat ttcgccgtgc gcgcggcaga actgctgaag caggggcaat
     5101 agccccgcca ggactttgta atgaacaccg aaggactcag tacccctgaa ttgcaag
//
LOCUS       DASRUM010000180         5254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_927095, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000180 DASRUM010000000
VERSION     DASRUM010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927095"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /gene="mmsB"
                     /locus_tag="VFF22_11605"
     CDS             <1..870
                     /gene="mmsB"
                     /locus_tag="VFF22_11605"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HZX17496.1"
                     /translation="FIGLGHMGLPMARNLLKAGFNLRVFDLLQAAVEDLAKDGAQAAS
                     SALDAVQDADVVVSMLPASRHVEGLYLGDNGLLAALKPGSLVLECSTIAPESARKVHQ
                     AAAARGIELLDAPVSGGTAGAAAGTLTFMIGGAATTLEKARAIFEAMGKNIFHAGPDG
                     AGQVAKVCNNQVLAVQMIATAEAMAMGVANGLEPAVLAEIMRQSSGGNWTLEKYNPWP
                     GVMENAPASKGYSGGFMAELMAKDLGLAQEAAQASGSSTPMGALALQLYRLLLKQGKG
                     KQDFSVVQQLFVD"
     gene            988..1326
                     /locus_tag="VFF22_11610"
     CDS             988..1326
                     /locus_tag="VFF22_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HZX17497.1"
                     /translation="MFYVQRDANGHLRRVEVMPFESMTDALTADSEEIQAWFATQNLE
                     SNLMQLKQSDLDMIRVVDDLVHLLISKGVIRITDLPAAVQAKLLTRSQARESLGGLHR
                     LINDDEHGII"
     gene            complement(1400..2551)
                     /locus_tag="VFF22_11615"
     CDS             complement(1400..2551)
                     /locus_tag="VFF22_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019826079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HZX17498.1"
                     /translation="MTDTQYSVAEGLTARLSPLAGMVMLAFLVAGLISVLWAMFVSDK
                     VALQPTQPLSWEQVFDGAVTHRIAEELAHVPFAKRAADLERAASWLAIGDTGTRVRQG
                     CANWLFLTDELKIHPQAAADAEARAQKVAALHEQLSKRGIRLLVVLVPDKSRIAAQQL
                     CAIERSPLLANRAQDWQRVLQAKGVEVLDLAPTLQPLGSEAFLRTDTHWSEAGAERSA
                     AAVAERIAALGVSPTPAKQFVASVTAPQPRPGDLVRLAGLDWLPEGLQPTMERVAVTQ
                     IKEVQGAADESALGEDDLFGDSQLPNLAVIGTSFSRNSNFIPFLERAVSARVGNFAKD
                     GGEFSGAAKDYFSSPAFKQTPPEVLIWEIPERDLQSPYRDDIVLDGQPG"
     gene            complement(2563..3978)
                     /locus_tag="VFF22_11620"
     CDS             complement(2563..3978)
                     /locus_tag="VFF22_11620"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003380351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HZX17499.1"
                     /translation="MVFTSLEFLTLFLPLVLLIYVLARPRWRNGVLLVGSWVFFGWLS
                     PMFMLLHTVLTVITWIGGLLIDRAPFGSKSRVRWLAGMIVLNVLVLCWYKYANILAAT
                     LNELLMSQGAMPLEWQKVALPAGLSFIVLQAISYLVDVHRRTVKVERSFIDYATYLLM
                     FGQSIAGPIIRYSWVSEELSNRGLSWRNFSLGARRFMIGMSMKVLVADSLAPVVDVAF
                     ALPEPSFVDAWMGCLAYSLQLFFDFAGYSAMAIGIGLMLGFHFPENFNRPYLARSIQD
                     FWRRWHLSLSSWIRDYLYIPFGGNRGSQWMIYRNLFLTMAIAGLWHGGDSWNFLLWGA
                     AHGVALCVARIWSGAGWFSMPRALSHVLTLLFVALAWTLFRAPDFATALTMYAGQFGM
                     HAFALGDALAATVRPTHGIAAALGVFCVIAPVYQQRLEARFSQNTLFCLLGSLWPVLG
                     FLLSFALIASRETVPFLYFQF"
     gene            complement(3989..4645)
                     /locus_tag="VFF22_11625"
     CDS             complement(3989..4645)
                     /locus_tag="VFF22_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003365724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042121 - alginic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate O-acetyltransferase AlgF"
                     /protein_id="HZX17500.1"
                     /translation="MRALFSLLALLPVSLLQAAEIPLYPTGPSEDSAFLRFFNAGDTA
                     LELSAENGASLRLEGDARASDFLTVPAGKPIKGSLKQGEAQQALDISVAPGEFASVVG
                     IATEQGLNLVTVREVPDDFNALKASLAFYSLDASCTNAGLQAAGRNVDIFKDVAYGAV
                     QRRSINPLSLSVQLRCAGASVGEPLALGELAAGQRYTLFLVPSADGPRLFQALDNLAN
                     "
     gene            complement(4645..>5254)
                     /locus_tag="VFF22_11630"
     CDS             complement(4645..>5254)
                     /locus_tag="VFF22_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019826084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZX17501.1"
                     /translation="HMSLQNHLTEPLNAALVAKGAKVHSIGACGASAADWLITKKVDC
                     GAVQDNTGKLVLKGSGATTTPIKQLIAQKKPDVVVLVIGDTMASYDKPAFPKAWAWQS
                     VTNLTKAIAETGTKCVWVGPAWGKVGGMYKKNDQRTQLMSQFLAANVAPCTYVDSLKM
                     SKPGQWVTTDGQHFTVAGYKAWGNAIAEAMAKLPAASLKGGK"
BASE COUNT         1180 a         1645 c         1527 g          902 t
ORIGIN      
        1 tttatcggcc tcggccacat gggcctgccc atggcgcgca acctgctcaa ggccggcttc
       61 aacctgaggg tcttcgacct gctgcaagca gccgtcgagg acctggccaa ggacggtgcg
      121 caagcggcca gcagcgcgct ggatgcggtg caggatgccg atgtggtggt cagcatgctg
      181 ccggccagcc ggcatgttga gggcctgtac ctgggtgata acggcctgct ggccgcgctc
      241 aagcccggca gcctggtgct ggaatgctcg accatcgccc cagaatccgc gcgcaaggtg
      301 catcaagcgg cggccgcgcg cggtatcgaa ttgctcgatg ccccagtatc cggcgggacc
      361 gccggcgcgg cagccggcac cctgaccttt atgatcggcg gcgcggcaac cactctggaa
      421 aaagcccggg cgatcttcga ggccatgggc aagaacatct tccacgccgg ccccgacggc
      481 gccggccaag tggccaaggt gtgcaacaac caggtgctgg cggtgcagat gatcgccacc
      541 gccgaagcca tggccatggg tgtggccaac ggtctggagc ccgcagtgct ggccgagatc
      601 atgcgccaga gttcaggcgg caactggaca ctggagaagt acaacccctg gcccggcgta
      661 atggaaaacg ccccagcgtc caaaggctac agcggcggtt ttatggccga actgatggcc
      721 aaagacctgg gtctcgccca ggaggccgcc caggccagcg gcagcagcac gccgatgggc
      781 gcactggcct tgcagctgta tcgcctgctg ctcaaacaag gtaaaggcaa gcaggacttc
      841 tcggtggtgc agcagctgtt tgtcgactaa accgaggatg atcggataca gcagggagcc
      901 tggttctata actggtcggg ggcgatgttc aagctaagct caatagcggg actcgccgag
      961 ggcgggtcat agggaaggat aatgccgatg ttctatgttc agcgggacgc caacggccat
     1021 ctgcgacgag tggaagtgat gccgttcgag agcatgaccg acgccctgac ggccgattca
     1081 gaagagattc aagcgtggtt tgccacccag aaccttgaat cgaaccttat gcaactgaag
     1141 caaagtgacc tggacatgat ccgtgtcgtg gacgatttgg tgcatttgct gatcagcaaa
     1201 ggcgttatcc gcatcacgga cctgcccgca gcggttcagg ccaagctact gacccgctct
     1261 caagccaggg aatcactcgg gggcctacac cgcctgatca atgatgatga gcacggcatc
     1321 atctaacagc gtcttaatgc accagaaaag caaaagatca tgctgtacag catggccttt
     1381 tatttgacag cccaaacctt caacctggtt ggccgtccag cacgatgtcg tcgcggtagg
     1441 gtgactgcaa atcacgctca ggaatttccc atatcagcac ctcaggcggt gtctgcttga
     1501 acgccgggct gctgaaatag tctttagccg cacccgagaa ctcgccgcca tccttggcaa
     1561 agttgcccac acgcgcactc actgcgcgct caaggaaagg gatgaagttg gagttacgtg
     1621 aaaacgaggt gcctatcacg gcaagattcg gcagctggct gtcgccgaac agatcgtctt
     1681 cacccagtgc agattcatcg gcagcgccct gcacttcctt gatctgcgtg acggcaacgc
     1741 gctccatcgt cggttgcaga ccttcgggta accagtcgag cccagccaaa cgcaccagat
     1801 cgccggggcg cggctgcggc gcggtaacac tggccacaaa ctgtttggcc ggagttgggc
     1861 taacgcccag tgcggctatc cgctcggcca ccgctgcagc tgatcgctca gcgccggctt
     1921 cgctccagtg cgtgtcggta cgcaggaagg cctcgctgcc gagtggttgc agggtcggag
     1981 ccagatccaa cacctcaacg cccttggcct gcagcacccg ctgccaatcc tgcgcacggt
     2041 tggccaacag tggcgagcgc tcaatggcac acaactgctg cgctgcaatg cggctcttgt
     2101 ccggtaccag cactaccagc aagcgaatgc cacgcttgct gagctgttca tgcaacgcag
     2161 cgactttctg cgcacgcgcc tcagcatctg cagcagcctg cgggtgaatt ttcagctcat
     2221 cggttagaaa cagccagttc gcacaacctt gacgcacccg ggtaccggta tcaccaatcg
     2281 ccagccaact ggcggcgcgc tcgagatcag cggcgcgctt ggcaaacggc acatgggcca
     2341 actcttcggc aatgcggtgg gtaaccgcgc catcgaaaac ctgctcccaa ctgagtggct
     2401 gcgtgggctg caacgccact ttgtcactga caaacatggc ccacagcaca gagatcaagc
     2461 ctgcgaccag gaacgccagc atcaccatgc ccgccagggg actcagccgc gcggttaacc
     2521 cttcggccac tgaatattgg gtatcagtca tgacatcagg ccttaaaact ggaagtacag
     2581 gaagggcacc gtttcacggc tggcaatcaa ggcaaacgac aacaaaaagc ccaataccgg
     2641 ccacagagaa cccaacaaac agaacagggt gttttggctg aagcgcgctt ccaaacgttg
     2701 ctgataaacc ggcgcgatca cgcagaacac acccaacgcc gccgcaatac catgagtcgg
     2761 gcgcacggtg gcggccaagg catcccccaa ggcaaaggca tgcatcccaa actggcccgc
     2821 atacatagtc aatgccgtgg cgaaatcagg ggcgcggaac aacgtccagg caagcgctac
     2881 aaatagcagc gtcagcacgt gtgaaagcgc tctgggcatg ctgaaccaac ctgcaccact
     2941 ccagatgcgc gccacgcaca gggccacgcc gtgggcggcg ccccagagca ggaaattcca
     3001 gctatctccc ccgtgccaca aaccggcaat ggccatggtc aggaacaggt tccggtagat
     3061 catccactgg ctgccacggt tgccgccaaa gggaatgtac aggtaatcac gtatccaact
     3121 ggacaacgac aagtgccagc gccgccagaa atcttgaata ctgcgcgcca ggtagggccg
     3181 attgaagttc tccggaaaat ggaaccccaa catcagacca ataccaatgg ccatggcgct
     3241 gtagccggca aagtcgaaaa acagctgcag cgaatacgcc aggcaaccca tccaggcatc
     3301 gacaaagctc ggctccggca acgcaaaggc aacatcgacc acaggcgcca ggctatcggc
     3361 caccaatact ttcatgctca tgccaatcat gaaacgccgg gcacccaggc taaagttgcg
     3421 ccagctgagc cctcgattgg acagctcttc actgacccag ctgtaacgaa tgatcggccc
     3481 ggcgatggac tggccaaaca tcagcaggta ggtggcatag tcgataaagc tgcgctcgac
     3541 cttgacggtg cgccgatgca cgtcgaccag gtatgaaatg gcttgcagca cgataaacga
     3601 cagccccgcc ggcagtgcca ccttttgcca ctccagcggc attgccccct gagacatcag
     3661 cagctcattg agggtcgctg ccaggatatt ggcgtacttg taccagcaca gcaccaggac
     3721 attgagcacg atcatcccgg ccaaccagcg cacacggctc ttcgagccga acggcgctcg
     3781 atcgatcagc aatccaccga tccaggtaat cacggtcagc accgtatgca gcagcatgaa
     3841 catcgggctg agccagccga agaacaccca actgccgacc agcaacacgc cgttgcgcca
     3901 ccgcggtcgc gcgagcacat agatcagcaa aacgaggggc agaaagagcg tcaggaactc
     3961 gagcgaggtg aagaccatgt tttatacgtc agttcgccag gttatcgagg gcctggaaca
     4021 ggcgcgggcc gtcagccgaa gggacaagga acagggtgta gcgttggccg gccgccaact
     4081 cacccagcgc cagcggctcg cccactgagg caccagcgca acgcagctga accgacaggc
     4141 tcaacggatt gatcgagcga cgctgcacgg cgccataggc aacatccttg aaaatatcga
     4201 cattacgccc ggctgcttgc aacccggcgt tggtgcagct cgcatcgagg ctgtagaacg
     4261 ccaacgaagc cttgagtgca ttgaaatcat ccggcacttc gcgcacggtg accaggttca
     4321 ggccctgctc tgtggcaatg ccaaccacgc tggcgaactc acccggcgcc acgctgatgt
     4381 ccagcgcctg ctgcgcctcg ccctgcttga gactgccctt gatcggcttg ccagccggca
     4441 cggtgaggaa gtcggacgcg cgcgcatcgc cttcaaggcg caggctggca ccattctccg
     4501 cactcagctc cagcgcggtg tcaccggcat tgaagaagcg caggaaagca gagtcttcac
     4561 tcgggccggt agggtacagg gggatctcgg cagcctgcag caggcttacc ggcagcagcg
     4621 caagcaagct aaacagtgcg cgcatcattt gcctccctta aggctggcag ctggcagctt
     4681 ggccatggct tcggcaattg cattgcccca ggccttgtag ccggccacgg tgaaatgctg
     4741 gccatcagtg gtgacccact gacctggctt ggacatcttc aatgaatcga cataggtgca
     4801 cggcgcaacg ttggcagcca ggaactgcga catcagctgg gtacgctgat cgtttttctt
     4861 gtacatgccg cccactttgc cccaagccgg ccctacccac acgcatttgg tgcctgtttc
     4921 agcaatcgcc ttggtcaggt tggtgacgct ctgccaggcc caggccttgg gaaacgctgg
     4981 cttgtcgtaa gaggccatgg tgtcaccgat gaccagcacc accacatccg gctttttctg
     5041 cgcaatcagc tgcttgattg gggtggtcgt cgcaccgctg cccttgagga ccagcttgcc
     5101 ggtattgtcc tgcacggcgc cgcagtcaac cttcttggtg atcagccaat ccgccgcact
     5161 ggcaccgcag gcgccgatgg agtgcacctt ggcacctttt gccaccaagg ccgcattgag
     5221 tggctcggtc aggtgattct gcaggctcat gtgg
//
LOCUS       DASRUM010000181        15656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1762516, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000181 DASRUM010000000
VERSION     DASRUM010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 15656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15656
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762516"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /gene="purM"
                     /locus_tag="VFF22_11635"
     CDS             complement(<1..624)
                     /gene="purM"
                     /locus_tag="VFF22_11635"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZX17502.1"
                     /translation="MSKQPSISYKDAGVDIDAGEALVERIKGVAKRTARPEVMGGLGG
                     FGALCEIPAGYKQPVLVSGTDGVGTKLRLALNLNKHDSIGQDLVAMCVNDLVVCGAEP
                     LFFLDYYATGKLNVDVAATVVTGIGAGCELAGCSLVGGETAEMPGMYEGEDYDLAGFC
                     VGVVEKAEIIDGSKVATGDALIALPSSGPHSNGYSLIRKIIEVSGTEI"
     gene            947..1996
                     /locus_tag="VFF22_11640"
     CDS             947..1996
                     /locus_tag="VFF22_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HZX17503.1"
                     /translation="MRLTARLLLVCLSLSGLPAFAAPVSDLYQVREAVSSQQPEERAA
                     ALSRALDTLVLRLTGKADAAQNPALAALRKDPQQIVSQYGYEGEQLLVDFDPQSTDRS
                     LRQAGLALWGANRPSILLWWLSDASDGSRLLGDGQAAAAPLNQAAQHRGLPLRLPLAD
                     LAEQLVAVPENLTANDPAALREISERYAADALLAVMAREANGQWQAEWRLWLGDEREQ
                     GTVQGADQAALADAILLAVNQRLAPRFIVAAGAASGLTVEVQGVDLARYAELQRILEP
                     FAAQLRKVQGDTLTFAVNANAEQLRAQLGLARLQEVPAEAPALDANQSITPPATAAEP
                     AATAVAPDNVLRFRW"
     gene            2121..3212
                     /locus_tag="VFF22_11645"
     CDS             2121..3212
                     /locus_tag="VFF22_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZX17504.1"
                     /translation="MIDSHRWIWIVGALLLCGLLYLLAPILSPFLIGVLLAYMGDPLV
                     DRLERWKLSRTWGVVVVFALITLIMAILLLVLVPMLGKQLVRLYELIPEMLDWAQNQA
                     LPWVQLKLGLNEGFWRFDQLKAALSGELGKTTDIVGMLLSTATSSGLALLAWLANLVL
                     IPVVSFYLMRDWDLMVAKLRSLLPRGREGLVVQLFGECHEVLGAFLRGQLLVMLALGV
                     MYATGLMVIGLELGLLIGVLAGLASIVPYLGVVVGIGAALTAGLFQFGMDPYPLVAIA
                     AVFIVGQMLEGMLLTPMLVGDRIGLHPVAVIFAIMAGGQLFGFTGVLLALPVAAVIMV
                     LVRHLHDFYKLSDLYGEPASGSDEPPNLV"
     gene            3209..3913
                     /gene="hda"
                     /locus_tag="VFF22_11650"
     CDS             3209..3913
                     /gene="hda"
                     /locus_tag="VFF22_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HZX17505.1"
                     /translation="MTPIQLPLGVRLRDDATFANYYPGANAAALGYVERLCEADAGWT
                     ESLIYLWGGEGVGRSHLLQAACLRFEQRGEPAVYLPLAEVVEYGPALLDNLEQCELVC
                     LDDLDAVAGRSDWEEALFHLFNRLRDSGRRLLLAACSSPRELPVQLADLQSRLTLALV
                     FQLQSLSDEDKLRALQLRASRRGLHLSDEVGRFILTRGERSMSALFELLERLDQASLQ
                     AQRKLTIPFLKETLGW"
     gene            complement(3923..4603)
                     /locus_tag="VFF22_11655"
     CDS             complement(3923..4603)
                     /locus_tag="VFF22_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZX17506.1"
                     /translation="MRLVLLPGLNGSNTLFGPLLAHLHPALEVQVLELPTEGSQAYKD
                     LADLLLGRLGNAPYVLLGESYSGPLAYLLALHRPPGLRGVIFAASFLSLPHPLLPQLK
                     YLPLPRWLLAQNTLLSLFCLNGQASTELTQLLREEIQKLPPRLLRARLYSLSQLQKPA
                     LQLELPTLHLLAKQDRLVTRRAQASLSACCTQLTEIVLAGPHFLLQTQPQACANTIQR
                     FIAGLPAD"
     gene            complement(4603..5013)
                     /locus_tag="VFF22_11660"
     CDS             complement(4603..5013)
                     /locus_tag="VFF22_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HZX17507.1"
                     /translation="MAKTAKPLPSLEWLQPRLNISRAVSLLSFFSLAALLLAWNLWFA
                     NVPSKLLWVILGVQLAPLLLLAPGLILGNARAHAWACFVVNLYFIQGVLAAFDPSKAL
                     YGWLLTGLSFSLFCAALMFTRWRFQFERKLAGES"
     gene            complement(5006..5611)
                     /gene="wrbA"
                     /locus_tag="VFF22_11665"
     CDS             complement(5006..5611)
                     /gene="wrbA"
                     /locus_tag="VFF22_11665"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018926302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HZX17508.1"
                     /translation="MSTPYILVLYYSRHGATAEMARQIARGVEMGGLEARLRTVPTVS
                     SECEAVAADIPAEGALYASLDDLKNCAGLALGSPTRFGNMAAPLKYFLDGTSGLWLTG
                     GLVGKPAGVFTSTASLHGGQETTLLSMLLPLLHHGMLITGLPYSEAALLETRGGGTPY
                     GASHHAGADGKRALDEHEIALCRALGQRLAQTAARLESARG"
     gene            complement(5608..5961)
                     /gene="arsC"
                     /locus_tag="VFF22_11670"
     CDS             complement(5608..5961)
                     /gene="arsC"
                     /locus_tag="VFF22_11670"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917501.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HZX17509.1"
                     /translation="MTAMTLYHNPRCSKSRSALQLLEDRGLSPTIVRYLDTPLNTAQL
                     QTLLDKLGISPRQLLRTGEDEYKTLGLSDPQLTDAQLIDAMVSQPKLIERPILVVGDK
                     AVVGRPPETILEILP"
     gene            6070..7299
                     /locus_tag="VFF22_11675"
     CDS             6070..7299
                     /locus_tag="VFF22_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HZX17510.1"
                     /translation="MLQRVNNLLEFWRFLLQRFIADRGTSNAAALTYTTLFAVVPMMT
                     VTFSMLSAVPAFQDTGEQIQSFIFRNFVPSAGETLQQYLRDFTSQARQLTWIGVAVLA
                     ATAFWMLVTIEKTFNTIWRVRQPRRGVSSFLLYWAILSLGPLLLGGGFAISTYFTSLS
                     LISGPDALLGAQVVLKFMPLLFSVAAFTLLYATVPNAQVPVRHALLGGLFAAVLFEVA
                     KMLFGLYVRLFPGYQLIYGAFATVPLFLLWIYVSWLIVLFGAELVCNLGFSQQWRKRA
                     VPRLLMALGVLRVFYERHQSGLKVRLLDVQRQGWSLAEHEWEEILVFLEQQKLIAPTG
                     SGTWVLSRDLGHYSLQQLLSHSPWPMPRAENLPEELDEVWYPALRSGLTKLQEEQAAL
                     FGEDLAKWLQPEAESEN"
     gene            7467..7583
                     /locus_tag="VFF22_11680"
                     /pseudo
     CDS             7467..7583
                     /locus_tag="VFF22_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244317.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7738..8130)
                     /locus_tag="VFF22_11685"
     CDS             complement(7738..8130)
                     /locus_tag="VFF22_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17511.1"
                     /translation="MTQALDKLVGPVPSAIVNLARLQVTPYEGRVAEFNVAAWLHLPG
                     LANLPVSLLISYRDGTTRREVMVDHGKVNQHSKILLSSVARLPVKLKIEEMQVRLRSA
                     VPATNLVVEELFVQAVELPQTDSRQALA"
     gene            complement(8270..9679)
                     /locus_tag="VFF22_11690"
     CDS             complement(8270..9679)
                     /locus_tag="VFF22_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZX17512.1"
                     /translation="MTLDALRLEVPDILETDVAPLADLNEQLAQARLQSPELALQQAL
                     GLLFRLNRSAMTLLERQRALQSFSDQYRHYSKAYQGENPPTALFVHLCSELAIGFKRL
                     LLQILQGRQPSRPHLAWCLYMAQHFLAQSLLRHYQLYQEPPPSLWRDSHLLYWIGEHQ
                     DCLDEPVAAAFTPQPANTMRGIYQQMLLLALSNPFHLADGECALLFGALSPLAGLARL
                     LPWDEDDEIEGPTIDLTEAQPCLSYAQTPEGSADCLRRFELGALLVALHDPAPLQSQA
                     ERQLLERVQQHWLGRQQRRHTRADHSSECNLMVGLPAIHAQLLEQRPLRTSAQMLDAS
                     AGGARLLCNADQAAQLPVGQLVLLITGNDTPTLALVRWRHLNSEGLHLGLRYLKGLPR
                     PVWLRRAPSAQTHPGVLQSTPAPGNGWHHGLWLPNGQFVEGENVWLQLASAHNQAILP
                     LPIANLVTASVTRYPMQLA"
     gene            9710..10189
                     /locus_tag="VFF22_11695"
     CDS             9710..10189
                     /locus_tag="VFF22_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZX17513.1"
                     /translation="MGMRFQAIIRAVAGVGIGLILAGCAEDIGVDQYGRKVAVERLEG
                     QWLVINYWAQWCAPCRTEIPELNALEQQLKEQSVQVLGVNFDALQGEKLSQAAQDMGI
                     NFTVLAQDPAGRYQLLRAEVLPVTYIVDDQGRMRERLLGEQTAAGLTARLTALKAED"
     gene            10193..10468
                     /locus_tag="VFF22_11700"
     CDS             10193..10468
                     /locus_tag="VFF22_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HZX17514.1"
                     /translation="MARTCMHGYVSGRVPGVCFRQATAEQAERLDLDGWVRNLADGRV
                     EVLFEGDETAVAELTSWLQHGPQAAEVTSLELQEQAQQGVAGFIVRR"
     gene            complement(10475..11047)
                     /locus_tag="VFF22_11705"
     CDS             complement(10475..11047)
                     /locus_tag="VFF22_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZX17515.1"
                     /translation="MLQRLTCALLGLLLCGTAYAEVRQAPEPELRTLLQQTVAQADSF
                     QDRFDAEVWLLDMSTRMRRYIPDAEERLSLLRLVHREASKAGLRPDLVIALIHAESHF
                     DRFAISSVGAQGMMQVMPFWKAELGRPQDNLTDNATNLRYGCTILSFYLRKENGDLNR
                     ALARYNGSLGKHTYSAKVVGFWHDFWFVKP"
     gene            complement(11055..12008)
                     /locus_tag="VFF22_11710"
     CDS             complement(11055..12008)
                     /locus_tag="VFF22_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17516.1"
                     /translation="MFKRNTFSLIATLVGGSLLLSGCANQLPQRSEHEERVERKLLDH
                     SLQIDVGEPRVLELPQRRVRVHEQKTFEVTAFDVTRRYDRYTPYQPWRELYEVPLGAL
                     AVVGGIGANVLNVVLLGSLPDSATKDWISYGFAGLNPAMNVESNGRSQQNLASLEESR
                     QDARMEYSSLPWAERPVRVKAGEQNHELLTDRNGILRLNLLDSPFAEEDVTLISTLLL
                     TVEDDQGSARADASLLVSRTLRGKLQEAHDLIYNDLEGDDVTQWVHRVKRLSELGLEE
                     EASELEQSLIELTRNDPELQKIFLQALTKDAGRLVADPGND"
     gene            complement(12035..13750)
                     /locus_tag="VFF22_11715"
     CDS             complement(12035..13750)
                     /locus_tag="VFF22_11715"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HZX17517.1"
                     /translation="MRTSQFLLSTLKETPSDAVVISHQLMLRAGMIRKLASGLYTWLP
                     MGLRVLRKVEKVVREEMNAAGALEVLMPGIQPAELWQESGRWEQYGPELLRMKDRHGR
                     DFCAGPTHEEVITDLARNELNSYKQLPINLYQIQTKFRDEIRPRFGLMRGREFIMKDA
                     YSFHVDQASLQQTYDRMHQAYCNIFTRLGLNFRPVQADTGSIGGTGSHEFHVLADSGE
                     DDIAFSDSSDYAANIEKAEAVPRESSRGAATESMRMVETPDTKTIDALVQGFGLAIEK
                     TIKTLMVHGAKKGTLVALIVRGDHELNEIKAANLELVASPLVFASEAEIRAAIGAGPG
                     SLGPVNLPIPCIIDRSVALMSDFASGANIDDKHYFGVNWERDLPLPEVADLRNVLAGD
                     PSPDGKGKLEIKRGIEVGHIFQLGTKYSEALNCQVLGENGKPVTLTMGCYGIGVSRVV
                     AAAIEQNNDDRGILWNDALAPFHIALVPLRYETDAVREATDKLYAELTAAGFEVLLDD
                     RDKKTSPGIKFADMELIGIPHRIVVSDRGLAEGNLEYKSRKETDAQAVPVADILSFIQ
                     ARISH"
     gene            13866..14273
                     /locus_tag="VFF22_11720"
     CDS             13866..14273
                     /locus_tag="VFF22_11720"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZX17518.1"
                     /translation="MTAEQVQALIRAGLPMAEDINLCIDRLDAKGALARVPFHGKLIR
                     PGGTLSGPTIMALADAAMYAVVLGSLGRVEMAVTSNLNINFLVKPKPVDLLAEARILR
                     LSRRQAVCEVSVYSHGNEEELVAHVTGTYALPL"
     gene            complement(14339..15574)
                     /locus_tag="VFF22_11725"
     CDS             complement(14339..15574)
                     /locus_tag="VFF22_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015802 - basic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family porin"
                     /protein_id="HZX17519.1"
                     /translation="MQVMKWSVIALAVAAGTTQIAMASDQSESKGFVEDSSLSLLARS
                     MYYNRDARNGASRGIRDNGYSEEWGGSARAIFESGFTQGTVGVGVNAYAGTLFQLDTG
                     TGRRGVGMFPENEGKDLTIANAAIKARISNTVLTYGSQMPALPVLAADDGRLLPETFE
                     GTMITSTEIEGLELNAGRFTAQNLMNQSGRDSFGLKSIDVAGGSYAFSDDFSAALYWS
                     DLEDRYERKYANLNYNLALAEEQSLGFDFNIYKSDYDRQFGGGDNTIWSLATKYTLGA
                     HGFIFAHQRNTGDRGFDYGVGDGGGSIFVANSFLSDFNLEDQRSWQASYELDMATFGV
                     PGLSLKTAYIWSDNIATGAGDDGKEHEFFNQVKYVVQDGAAKDLSFRLRSSVLRSNSE
                     VGTDTNEVRAYIEYPLSVL"
BASE COUNT         2873 a         4271 c         5031 g         3481 t
ORIGIN      
        1 gatttcagta ccggacactt cgatgatctt gcggatcagc gagtagccgt tggagtgcgg
       61 gccggaggac ggcagggcga tcagcgcgtc accagtggcg actttcgaac cgtcgatgat
      121 ttcggctttt tccaccacgc caacgcagaa gccggccagg tcgtagtctt cgccttcgta
      181 catgccgggc atttcagcgg tttcaccgcc aaccagcgag cagcccgcca gttcgcagcc
      241 ggcgccgatg ccggtgacta cggtggcggc cacgtcgacg ttgagcttgc cggttgcgta
      301 gtagtcgagg aagaacagtg gctcggcgcc gcaaaccacc aggtcgttaa cgcacatggc
      361 gaccaggtcc tggccgatgc tgtcgtgttt gttcaggttc agcgccaggc gcagcttggt
      421 gcccacgccg tcggtgccgg agaccagtac cggttgcttg tagccagccg ggatctcgca
      481 cagcgcgccg aagccgccca gcccacccat gacttccgga cgcgcggtgc gcttggccac
      541 gcctttgatg cgttcgacca gggcttcgcc cgcgtcgata tctacacctg cgtccttgta
      601 gctgatggag ggttgcttgc tcatagatcc aggcctttag gggaaattcg gattggcacc
      661 accgcttgtg gcggcggtct gcgaaggcgc gcgattttat caggcttgcc cggcagcggc
      721 cacccgcgaa ggttgcggct gttaggcgtg ctgtttaagg tagccacttg ctcagaatct
      781 ggtcgctgtt ggcgattcga ggctgaaaag ggctttggaa tgcgccttaa cagcacctaa
      841 actcgcactc ttgagtcagc gtcgcctgcc ttatatggcg tgggttggcc gcaactattg
      901 ttaaacctgg cctgcgcgcc actttccgtc cgtcgagaat tcatccatgc gtttgaccgc
      961 tcgcctgctt ctagtctgtt tgtcgctgtc cggcctgccg gcgtttgccg cgccggtgag
     1021 tgatctgtac caagtccgcg aagcggtcag cagccagcaa ccggaagagc gcgctgccgc
     1081 cctgagccgc gcgctggata ccctggtatt gcgtctgacc ggcaaggccg atgcagcgca
     1141 gaacccggca ctggcggcgc tgcgcaagga cccgcaacag atcgtcagcc aatatggtta
     1201 tgagggtgag cagctgctgg tcgactttga cccgcagagc accgatcgca gcctgcgtca
     1261 ggcgggcttg gcgctgtggg gcgccaatcg tccgagcatt ttgctgtggt ggctgagcga
     1321 tgccagcgac ggcagccgtc tgctcggtga tggtcaagcc gctgccgcac cgctcaatca
     1381 ggccgcgcag caccgtggac tgccgctgcg cctgccgttg gctgatctgg ccgaacaact
     1441 ggtggcagtg ccggaaaacc tcacggccaa tgatccggct gccctgcgtg aaatctccga
     1501 acgctacgcc gccgatgcct tgctggccgt tatggcgcgc gaagcaaacg gccaatggca
     1561 ggccgagtgg cgcctgtggc tgggcgatga gcgggagcag ggcactgtgc aaggtgccga
     1621 tcaagccgcg ttggccgatg ccatcctgtt ggcggtcaac cagcgtctgg caccccgctt
     1681 tattgtcgcg gctggcgccg ccagtggtct taccgtggaa gtgcagggcg ttgacctggc
     1741 gcgttatgcc gagctgcagc gcattcttga gccgttcgcg gcgcaactgc gcaaggttca
     1801 gggcgatacg ctgacctttg ccgtcaatgc caatgccgag cagttgcgtg cgcaactggg
     1861 cctggcgcgc ttgcaggaag tgccggcaga agcccctgcg ctcgatgcta accagtcgat
     1921 tacgccgccg gctactgcgg ctgagcccgc tgccacagcg gtagcccctg acaatgtgct
     1981 gaggtttcgc tggtagactg cggctattac acccggtccc tgacttgaaa accgcagcta
     2041 tcgagctgcg gttttcttat gtaacgggcc tcgcggtttg agtctttctg gataaaacgt
     2101 tggcatatgg agcgctgggc atgatcgatt cacatcgttg gatatggata gtcggggcgc
     2161 tgttgctgtg cggcctgttg tacctgctgg cgccgattct ctcgccgttt ctgatcggcg
     2221 tgctgctggc ctatatgggc gacccgctgg tcgaccgtct ggagcgctgg aaactctcgc
     2281 ggacctgggg cgtggtggtg gtctttgcgc tgatcaccct gatcatggcc atcctgctac
     2341 tggtattggt gcccatgctg ggtaagcagc tggtgcgctt gtacgagttg atcccggaga
     2401 tgctcgactg ggcacagaac caggctctgc catgggttca gctcaaactg ggtctgaacg
     2461 aaggcttctg gcgctttgat cagctgaaag ccgcgctgtc cggggaactg ggcaaaacca
     2521 ccgatatcgt cggcatgctg ctgtccacgg ctacctcttc aggcctggcg ctgctggcct
     2581 ggctggccaa tctggtactg attccggtgg tgagcttcta cctgatgcgc gattgggacc
     2641 ttatggtcgc caaactgcgc agcttgctgc cgcgtggtcg tgaaggcctg gttgtgcagc
     2701 tgttcggtga gtgtcacgaa gtgctcggcg ccttcctgcg cggtcagttg ctggtgatgc
     2761 tggcgctggg tgtgatgtat gccaccggcc tgatggtgat tggcctggag ctcggactgt
     2821 tgatcggcgt actggccggc ttggccagca tcgtgccgta tttgggtgtg gtggtcggca
     2881 tcggtgcggc attgacggcc ggcctgtttc agttcggcat ggacccttac ccactggtgg
     2941 ccatcgctgc agtctttatc gtcgggcaaa tgctcgaagg gatgctgctg acaccgatgc
     3001 tggtgggcga ccgcattggc ctgcacccgg tagcagtgat cttcgcgatc atggccggcg
     3061 gccaactgtt cggctttact ggcgttctac tggccttgcc agtggctgcg gtgattatgg
     3121 tgttagtgcg tcatctgcat gatttctata aactttccga tctttacggt gagccagcca
     3181 gcggttccga cgagccgccc aacctagtat gacccccata caactgcctt taggtgtgcg
     3241 tctgcgtgac gacgccactt tcgccaatta ctaccccgga gctaacgctg cggcgttggg
     3301 ttatgtcgag cgtctgtgcg aggccgatgc cggctggacg gaaagcctga tctatctgtg
     3361 gggcggtgag ggcgtagggc gaagtcactt gttgcaggca gcctgtttgc gctttgagca
     3421 gcgtggcgag ccggcggtat acctgccgtt ggctgaggtg gtcgagtacg gcccggcgct
     3481 gctcgacaat ctggagcagt gcgagctggt gtgtctggat gatctggatg cggtggctgg
     3541 gcgcagcgac tgggaagagg cgttgtttca tctgtttaat cgcctgcgcg acagtggtcg
     3601 gcggctgtta ctggccgcct gcagctcgcc gcgcgaattg ccggtgcaac tggctgatct
     3661 gcagtcgcgc ctgacattgg cgctggtgtt tcagctgcaa tcgctgtccg acgaggacaa
     3721 gctgcgcgcc ttgcaactac gtgcttcgcg tcgcgggctg cacctgagtg atgaagtcgg
     3781 gcgttttatc ctgactcgcg gcgagcgcag tatgagcgcg ttgttcgagt tgcttgagcg
     3841 tctggatcag gcgtcgttgc aagcccagcg caagctgaca attccctttc ttaaagaaac
     3901 tctcggctgg tagtaggcct gtctagtctg ccggcagccc ggcaataaag cgctgtatag
     3961 tgtttgcaca ggcctgtggt tgggtctgca aaagaaaatg cgggccagcc aatacgatct
     4021 ctgtcagctg cgtacagcat gccgacagac tggcctgggc tctgcgggtt accagccggt
     4081 cttgcttggc cagtaggtgc agtgtgggca attcgagctg caatgcaggt ttctgtaatt
     4141 ggctaaggct gtacaaacgt gctcgtagca aacgcggtgg tagcttctgg atttcttcac
     4201 gcaataactg ggtcagctcg gtgctggctt ggccatttag gcagaacagg cttaacaagg
     4261 tgttttgcgc aagtaaccag cgaggcaacg gtaggtactt gagctgcggc agtagtggat
     4321 gcggcagact caagaatgag gcggcgaaga tcacgccccg caatccaggt ggccgatgta
     4381 gtgccagcag gtaggccagt ggaccggaat acgattcgcc aagcagcaca tagggtgcat
     4441 tgccgagcct acctaacaat agatcggcca aatctttata ggcttggctg ccttcagtag
     4501 gcagttctag cacctgcact tccagcgctg gatgcagatg agccagtaaa ggtccgaaca
     4561 gggtgttgct gccgttgagg cctggcagca acaccaagcg catcaggatt cccctgccag
     4621 tttacgctcg aactgaaaac gccagcgggt aaacatcagt gcggcgcaga acaggctgaa
     4681 gctcagccct gtcagcagcc agccatacag cgccttgctg gggtcgaagg ccgcgagtac
     4741 gccctggata aagtacagat tgaccacaaa acaggcccag gcgtgggcgc gggcgttacc
     4801 taggatcagg ccgggggcga gtaacagaag aggcgcgagt tgtacgccga ggatcaccca
     4861 gagcaatttg ctggggacgt tggcgaacca caggttccac gccagcagca acgcggcgag
     4921 gctgaaaaaa ctcaatagac tgaccgcgcg gctgatgttc aggcgcggtt gcaaccattc
     4981 cagggacggc agcggttttg cggttttagc cacgggcgct ctccagtctt gcggcggttt
     5041 gcgccaggcg ctggccgagt gcgcggcaca gggcaatttc atgttcatca agagcgcgtt
     5101 tgccatcagc accggcgtgg tggctggcgc catagggcgt gccgccgcca cgggtttcca
     5161 gcaacgcagc ttcgctgtag ggcaggccgg taatcagcat gccgtggtgc agcagtggca
     5221 gcagcatcga cagcagggtg gtttcctggc cgccgtgcag gctagcggtg gaagtgaata
     5281 cgccggcggg tttgccgact aggccaccgg ttagccacag gccgctagtt ccatcaagga
     5341 agtacttgag cggagcggcc atattgccga agcgggtcgg gctgccgagt gccaggccag
     5401 cgcagttctt caggtcatca aggctggcgt acagcgcgcc ttcggctggg atatcggcag
     5461 ccactgcttc gcattcgctg gagaccgtcg gtactgtgcg caagcgcgct tccaggccgc
     5521 ccatttctac accacgggcg atctgccggg ccatttctgc agtggcgcca tgacggctgt
     5581 aatacagcac caggatgtaa ggcgtgctca cggcagaatc tccagaatgg tttcaggtgg
     5641 gcgaccgacg acggctttgt cgccgacgac cagaatgggt cgctcaatta attttggctg
     5701 gctgaccatg gcgtcgatca gctgtgcgtc agtcagttgc gggtcgctca ggcctagggt
     5761 tttgtattca tcttcgccag tgcgcagcag ttgccgtggg ctgatgccga gcttatccag
     5821 cagagtctgc agctgggcgg tgttcagcgg cgtgtccagg taacgcacga tggttgggct
     5881 gagcccacgg tcttcgagca attgcaatgc actgcgcgat tttgagcagc gcgggttatg
     5941 gtagagcgtc atagcggtca tgttcgggtc gcctgttacc ttcgatggcg gctattctaa
     6001 ccgctaacgg cactcacgcc tgcgcctctg tagcacaggt gccgggatgc aaatggctaa
     6061 ggagagggca tgctgcagcg tgttaacaat ctgttggagt tctggcgctt tttgttgcag
     6121 cgctttattg ccgaccgagg caccagtaat gccgcggcct tgacctacac caccctgttt
     6181 gcggtggtgc cgatgatgac ggtgaccttt tccatgctct cggctgttcc ggcgtttcag
     6241 gataccggcg agcagattca gagctttatc ttccgcaact ttgtgccttc tgccggtgaa
     6301 accttgcagc agtatctgcg tgatttcacc agccaggcgc gtcaactgac ctggattggt
     6361 gtggcggtat tggccgcgac ggcgttctgg atgctggtga ccatcgagaa gaccttcaac
     6421 accatctggc gcgtgcgtca gccgcggcgt ggtgtgtcga gctttttgct gtactgggcg
     6481 attctcagcc tggggccgtt gttgctcggt ggcggttttg ccatcagcac ttactttact
     6541 tctctgtcgc tgatttccgg gccggatgcc ttgctcggag ctcaggtcgt tctgaagttt
     6601 atgccgctgc tgtttagcgt ggcggcgttc actctgctgt atgccacggt gccgaatgct
     6661 caggtgccgg tgcggcatgc gctgctaggc gggttgttcg cggcagtgct gttcgaagtg
     6721 gcgaagatgc tgttcggcct gtatgtgcgc ctgtttcctg gttatcagct gatttacggc
     6781 gcgtttgcca cggtgccgct gtttctgttg tggatctacg tgtcttggct gatcgtgctg
     6841 ttcggcgccg aactggtctg caacctgggc ttttcccagc aatggcgcaa gcgtgcggtg
     6901 ccgcgtttgc tgatggcgct tggtgtgttg cgggtgttct acgagcgtca tcagtcgggt
     6961 ttgaaagtgc gcttgctgga tgtgcagcgt cagggctggt cgctggctga acatgagtgg
     7021 gaagagattc tggtttttct cgagcagcag aaactgatcg ctcccacagg ttccggcacc
     7081 tgggtgttga gccgcgacct cggtcattat tcgctgcagc agctgcttag tcatagtccc
     7141 tggccgatgc ccagggcgga gaatttgccc gaggaactcg atgaggtctg gtatccagcg
     7201 ctgcgcagcg gtttgacgaa gttgcaggaa gagcaggcgg cgctatttgg tgaagacctg
     7261 gcgaagtggt tgcagccaga ggctgagtcg gaaaactgac gtaaaacgtc agtttgcggc
     7321 gttgcgctga tggcgcatga tggcactggc aaccatgaag gtcgccaaac gcggttaaaa
     7381 ggaagctggc acgcttctgg ctatttgtct tgcagatagc gatcaggttg ccagcagggc
     7441 agggattggc ggagtgtgaa ggcgttatga cagccactaa aggcaaagtg attcatctat
     7501 accagcgtga cccgcaagag gccctcgagc gccttaatcg catcactggc ctgcgcttta
     7561 gcagctggcc acagtctttg gtcaattcca acccgtctga gcagctcaac tcttctggct
     7621 catctgaaac tgttgcgcga cctgattctg acacccagtg cgggcctgca ggaatactct
     7681 cgcgctaaac gcaaaacccc gcgtcagtgc gcggggtttg tgtgtagtgg ggtgctatca
     7741 ggcgagagct tggcggctat cggtttgtgg caactcgacc gcttgcacaa acagctcctc
     7801 gaccaccagg ttggtcgccg gcacggcaga gcgcagacgc acctgcattt cttcgatctt
     7861 cagcttcacc ggcaggcggg ccacgctgga aagcagaatc ttgctgtgct gattgacctt
     7921 gccgtggtca accatcactt cacggcgggt ggtgccgtcg cgatagctga taagcaggga
     7981 cacaggcagg ttggccagcc ccggcaagtg cagccaggcg gccacgttga actcagcgac
     8041 gcgcccttcg tacggggtga cctgtaagcg ggcgagattg acgatggcag atggcacggg
     8101 gccgaccaac ttatcgaggg cttgggtcat ggtggtgttc caggctaata acggatcgca
     8161 tcaagcgtga gtgcgatgaa gtataagctt ggctcgtccg atgaaactgt gcgctcggcc
     8221 ggcgttcggc gtgacctttc tcgcagtttt agcgtatcgg caacaaggct taggccagct
     8281 gcatcgggta gcgagtgaca gatgcggtga ccagattggc gatcggcagc ggcaggatcg
     8341 cctggttgtg tgcgctggcc agttgcagcc agacattttc cccttcaaca aactgcccat
     8401 tcggcagcca taggccatgg tgccagccgt tgcccggtgc cggcgtgctc tgcagtacgc
     8461 ctgggtgagt ctgggcgctg ggtgcgcgcc gtagccatac cggacgcggc aggcctttca
     8521 ggtagcgcag gccaaggtgc aggccttcgc tattcagatg ccgccagcgc accagggcca
     8581 gggtcggcgt atcgttgccg gtgatcaata gcacaagctg gccaaccggc agctgcgcgg
     8641 cctggtcggc attacacagc aagcgtgcgc cgcccgcact ggcgtcgagc atctgcgcgc
     8701 tggtgcgcag tggccgttgt tccagcaact gtgcatgaat agcgggcagg ccgaccatca
     8761 ggttgcattc cgagctgtgg tcggcgcggg tatgtcgacg ttgctggcgc cccagccagt
     8821 gttgttgcac gcgttccagc agttggcgtt ccgcctgact ttgtagcggt gcgggatcat
     8881 gcagggccac cagcaaggcg cccagctcga agcggcgcaa acagtcagcg ctgccttctg
     8941 gcgtctgggc gtagctcagg cagggttgcg cctctgtcag gtcgatggtc ggtccttcga
     9001 tttcatcgtc ctcatcccat ggcagcaggc gcgccagccc agctagcggc gaaagagcgc
     9061 cgaacagcag cgcacattcg ccatcggcaa ggtgaaacgg gttgctcagc gcgagcagca
     9121 gcatttgctg gtagatgccg cgcatggtgt ttgccggttg tggggtaaac gccgcggcta
     9181 ctggttcatc caggcagtct tgatgttcgc cgatccagta cagcagatgg ctatcgcgcc
     9241 acagactggg cggcggctcc tgatacaact gatagtggcg cagcaggctc tgcgcgagaa
     9301 agtgctgggc catatacagg caccaggcca ggtgtgggcg tgatggctgg cggccctgga
     9361 ggatttgcag gagcaggcgt ttaaagccga tagccagttc gctgcacagg tgcacgaaca
     9421 aggccgtagg tggattctcg ccctggtagg ccttactgta atggcggtac tgatcgctga
     9481 agctttgcag tgcgcgctgc ctttcgagta aggtcatggc gctacggttc agcctgaaga
     9541 gcaggccgag cgcctgctgc aaggccagct ctggcgactg caggcgggct tgggccagct
     9601 gctcattaag gtcggctagt ggcgcgacat cggtttcgag gatgtctggc acttccaggc
     9661 gcaaggcatc taatgtcata tcaaccttat ggaatcaggg agcgaatgca tgggaatgcg
     9721 tttccaggcg atcatccgtg ctgtggcggg cgtaggcata ggtctgattc ttgccggttg
     9781 cgctgaagat ataggcgtcg atcaatatgg acgaaaggta gcggttgagc gcctggaagg
     9841 ccagtggtta gtgattaatt actgggcgca gtggtgcgca ccttgtcgca ccgagattcc
     9901 cgaactcaat gccttggagc aacagctcaa ggagcaatcc gtgcaggtgc tcggggtgaa
     9961 tttcgatgcg ctgcagggtg agaagttgag tcaggccgcg caagacatgg gcatcaactt
    10021 taccgtgctg gcgcaggacc cggccgggcg ctatcagttg ctgcgtgccg aagtgctgcc
    10081 ggtgacctat atcgtcgacg atcagggccg tatgcgtgag cgcttgctcg gtgaacagac
    10141 ggctgctggt ctgacggctc gcctcactgc actcaaggcg gaggactaag ccgtggcgcg
    10201 tacctgcatg catggttacg ttagcggccg cgttccgggt gtgtgctttc gccaggcaac
    10261 ggcggagcag gctgagcggc ttgatctcga tggttgggtg cgcaacctgg ccgatggccg
    10321 ggtcgaagtg ctgttcgagg gtgatgaaac ggcggtggct gaattgacga gctggttgca
    10381 acacgggccg caggctgctg aagttacctc actggagctg caagagcagg cgcagcaggg
    10441 ggttgccgga tttatcgtgc ggcgctgaat ctgttcacgg tttgacgaac cagaagtcat
    10501 gccagaaacc gaccaccttg gccgagtagg tgtgtttgcc cagactgccg ttatagcgcg
    10561 ccagtgctcg gttcaggtcg ccattttcct tgcgtaaata gaagctgagg atggtgcagc
    10621 cataacgcag gttggtggca ttgtcggtga ggttgtcctg tgggcggccc agctcggctt
    10681 tccagaaggg catcacctgc atcatgccct gagcgccgac tgaggagatg gcgaagcggt
    10741 cgaaatggct ttcggcatga atcagtgcaa ttaccagatc tgggcgcaag ccggctttgc
    10801 tggcttcgcg atgcaccagg cgcagcaggc tcaggcgttc ctcggcatcc gggatatagc
    10861 ggcgcatgcg cgtggacata tccagcagcc agacttcggc atcgaagcga tcttggaagc
    10921 tgtcggcctg ggccacggtc tgttgcagca gtgtgcgcag ttccggctct ggcgcctgac
    10981 gcacttcggc ataagccgtg ccgcagagca gcaggccgag tagggcgcag gttaggcgct
    11041 gcagcatggg gacgtcagtc gttgccagga tcggcgacca ggcggccggc gtctttggtc
    11101 agggcctgga ggaagatttt ctgcagttcc ggatcgttgc gtgtcagctc gatcaggctc
    11161 tgttccagct cgctggcctc ctcttcgagg cccagttccg ataggcgttt gacccgatgc
    11221 acccattggg taacgtcatc accctcaagg tcgttgtaga tcaggtcgtg ggcttcctgc
    11281 agcttgccgc gtaaggtgcg actgaccagc agggaggcat cggcgcgcgc cgagccttga
    11341 tcatcttcaa ccgtaagcaa cagcgtgctg ataagggtga catcttcttc ggcaaacggg
    11401 ctgtcgagca ggttcaggcg caggatgccg ttacggtcgg tgagcagttc gtggttctgt
    11461 tcgccggctt taacccgcac cgggcgctct gcccacggca ggctggagta ttccatgcgg
    11521 gcatcctggc gactctcctc aaggctggcg aggttctgct gcgagcggcc gttggactcg
    11581 acgttcattg ccgggttgag cccggcaaag ccgtagctga tccagtcctt ggtggcgctg
    11641 tcgggcagac tgccgagcaa taccacgttc aacacgttgg cgccgatacc gccgaccacc
    11701 gccagtgctc ccagcggcac ttcatacagt tcgcgccaag gctggtaagg ggtgtagcgg
    11761 tcatagcggc gcgttacgtc aaacgccgtg acctcgaaag tcttctgctc atgcacgcgc
    11821 acgcggcgtt gcggcaactc cagcacgcgt ggctcaccga catcgatctg caggctgtga
    11881 tcgagcagtt tgcgctcgac gcgctcctcg tgttcgctgc gttgcggcaa ctggttggcg
    11941 caaccgctga gaagcagaga gccacccaca agggtggcga tgaggctgaa ggtgtttcgc
    12001 ttgaacatga cgactcttgc taaaggggct gagatcagtg gctgatacgg gcctggataa
    12061 agctcaggat gtcggcgaca ggcacggcct gggcgtcggt ttccttgcgg ctcttgtact
    12121 ccaggttgcc ttcggccagg ccacgatcgc tgactacgat gcgatgcggg atgccgatca
    12181 gttccatatc cgcgaatttg atgcccgggc tggtcttttt gtcacggtca tcgagcagta
    12241 cttcgaaacc ggcagcggtc agctcggcat atagcttgtc ggtggcttcg cgcacggcgt
    12301 cggtctcgta acgcaatggc accagggcga tatggaaagg cgccagagcg tcattccaaa
    12361 gaatgccgcg atcgtcattg ttctgctcga tggctgctgc caccacgcgg gaaacgccga
    12421 tgccatagca gcccatggtc agggtcaccg gcttgccgtt ctcacccagc acctggcagt
    12481 tcagcgcttc gctgtacttg gtgcccagct ggaagatatg cccgacttcg ataccgcgtt
    12541 tgatttctag cttgcctttg ccatctgggc tcgggtcgcc agccagtaca ttgcgcaggt
    12601 cggcaacttc cggcagcggc aggtcgcgct cccagttgac gccgaaataa tgcttgtcgt
    12661 cgatattggc gccagaggcg aagtcgctca ttagggcaac cgagcggtcg atgatgcaag
    12721 ggatcggcag attgaccggg cccagtgagc ctggacctgc gccgatagct gcgcgaatct
    12781 cggcttcgct ggcgaaaacc agcgggctgg caaccagctc caggttggcg gctttgatct
    12841 cattgagttc gtggtcgccg cgtacgatca gggcgaccag ggtgcctttt ttcgcgccgt
    12901 gcaccatcag ggtcttgatg gttttctcga tggccaggcc aaagccctga accagcgcgt
    12961 cgatggtctt ggtgtccggg gtttcaacca tgcgcatgct ttcagtcgcc gcgccacggc
    13021 tgctttcgcg cggaaccgct tcggctttct cgatattggc ggcgtaatcg gagctgtcgc
    13081 tgaaggcgat atcgtcttcg ccggagtcgg ccaatacgtg gaactcgtgg gagccagtgc
    13141 caccgatgga gccggtatca gcttgcactg ggcggaagtt caggcccagg cgggtgaata
    13201 tgttgcagta ggcctggtgc atgcggtcgt aggtttgctg caaggacgcc tgatcgacgt
    13261 ggaaggagta ggcgtccttc atgatgaact cgcgtccacg catcaggccg aagcgcgggc
    13321 ggatttcgtc acggaacttg gtctggatct gatacaggtt gatcggcaac tgcttgtagc
    13381 tgttcagctc gttacgtgcc agatcggtga tgacttcttc atgggtcggg ccggcgcaga
    13441 aatcgcggcc gtggcgatcc ttcatgcgca gcagttctgg gccgtactgt tcccagcgac
    13501 cggattcctg ccacagctct gctggttgaa tgccaggcat cagtacttcc agcgcgccag
    13561 cggcattcat ttcctcgcgc acaactttct caaccttgcg cagcacgcgc aggcccatgg
    13621 gcagccaggt atacaggccg gaagccagct tgcggatcat gccggcacgc agcattagct
    13681 ggtggctgat gaccacggca tcggatgggg tttctttcag agtcgagagc aggaactggc
    13741 tggtacgcat atttggctgt tctgtcggtt gcaagagatt ttgaatggcc cgcattgtac
    13801 ggtgcctgta cagatgcgta caggatgctt gcgtgtgctt ataaaaggag gggagtgtgt
    13861 cgatattgac ggcggaacag gtgcaggcgc tgattcgggc agggctaccg atggcagaag
    13921 atatcaacct gtgtattgat cgattggatg ctaagggtgc actggcgcgg gtgccatttc
    13981 acggcaagtt gattcgaccc ggcggcacct tgtccggccc gaccattatg gccctggcag
    14041 atgcggcgat gtatgcggtg gtgctgggca gtctcggtcg ggtggaaatg gctgtgacat
    14101 ctaacttgaa tatcaacttc ctggttaagc ccaagccagt tgatctattg gcagaggcgc
    14161 ggatattgag attgagtcgc aggcaggctg tttgcgaagt atccgtatat tcgcatggta
    14221 atgaagaaga gttggtggca catgtaacgg gaacttacgc gttaccgttg taagttttaa
    14281 gctggcaaga aaaagcccga cctaggtcgg gcttttctta ccagctaaac ggtcttagtt
    14341 acagaacgct cagtgggtat tcgatgtaag cacgcacttc gttggtatcg gtacctactt
    14401 ccgagttgct gcgcagtacg gaggagcgaa ggcggaagga cagatccttc gccgcaccat
    14461 cctggactac gtatttaact tggttgaaga actcatgctc tttaccatcg tcgcctgcac
    14521 cagtagcaat gttgtcagac cagatgtagg cagttttgag gctgaggcct ggtacgccaa
    14581 atgtagccat gtccaattca tagctagctt gccaggagcg ctgatcctct aggttgaagt
    14641 ctgagaggaa gctgttggct acgaaaatag aaccaccgcc atcgccaacg ccataatcaa
    14701 agccgcgatc gccggtgttg cgttggtgtg caaatataaa gccgtgagca cctaaagtat
    14761 atttggtcgc caagctccag atggtgttgt caccaccgcc gaattgacga tcatagtcgc
    14821 tcttgtaaat gttgaaatca aaaccaagag actgttcttc agcaagagcc agattgtagt
    14881 ttagatttgc atacttgcgc tcatagcggt cttcaaggtc actccagtaa agtgccgcac
    14941 tgaagtcatc gctgaaagca taggagccac cagctacgtc gatagatttg aggccaaagc
    15001 tatctctgcc ggactgattc atgagatttt gcgcagtgaa gcgaccagca ttgagttcaa
    15061 ggccttcgat ctcggtgctg gtgatcatgg tgccttcaaa ggtttcaggc agcaggcggc
    15121 catcatcggc ggccaatacc ggaagcgccg gcatttgcga gccgtaggtc agtactgtgt
    15181 tggagatgcg ggctttgatc gcagcgtttg cgatggtcag gtctttccct tcattttccg
    15241 ggaacatgcc gacgccgcgg cgaccggtgc ctgtgtccag ctggaacagg gtgccagcat
    15301 aagcattcac accaacacct acagtgcctt gggtgaagcc ggattcaaaa attgcgcggg
    15361 cgctgccgcc ccattcttca gaataaccat tgtcgcggat accgcgagaa gccccattac
    15421 gagcatcgcg gttgtaatac atgctgcgcg cgagaagcga caggctgctg tcttcgacaa
    15481 agcctttgga ttctgattga tcagaggcca tggcaatttg ggtggtgccc gctgctactg
    15541 cgagggcgat tacgctccac ttcatcactt gcatcgtgat tgctcctttg gtttagaaaa
    15601 gttgcgccgt ccggctggtt gttatatgga cgactctttc tttttgtgtc ggcggt
//
LOCUS       DASRUM010000182         5879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2262157, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000182 DASRUM010000000
VERSION     DASRUM010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2262157"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFF22_11730"
     CDS             <1..576
                     /locus_tag="VFF22_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HZX17520.1"
                     /translation="GVVEKAEIIDGSKVATGDALIALPSSGPHSNGYSLIRKIIEVSG
                     TEIETTQVDGKPLTDLLMAPTRIYVKPLLKLIKDTGAVKAMAHITGGGLLDNIPRVLP
                     KGAQAVVDVASWTRPAVFDWLQEKGNVDEHEMHRVLNCGVGMVICVAQDQVEVTLQTL
                     TAAGEQPWVIGQIGEAAEGAAQVVLNNLKQH"
     gene            588..1238
                     /gene="purN"
                     /locus_tag="VFF22_11735"
     CDS             588..1238
                     /gene="purN"
                     /locus_tag="VFF22_11735"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZX17521.1"
                     /translation="MPTDCNVVVLISGSGSNLQALIDSIVHDSNPARIAAVISNRVDA
                     YGLERAKQAGIDTAVLDHKQFDGREAFDAALVEAIDAFNPQLVVLAGFMRILTPGFVR
                     HYAGRLLNIHPSLLPKYKGLHTHQRALDAGDSEHGCSVHFVSEELDGGPLVVQAVVPV
                     QSDDSPTSLAQRVHEQEHVIYPLAVRWFAEGRLQLTQQGAMLDEQLLPGTGHLIRT"
     gene            1247..1963
                     /locus_tag="VFF22_11740"
     CDS             1247..1963
                     /locus_tag="VFF22_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HZX17522.1"
                     /translation="MGRALLFALTLLSLPALAVELKPFSASYTADWKQLPVSGSAERS
                     LKTLDNGLWQLNFEASMLVASLTEQSTLRVDKEVFLPQTYLYERNGLGKGKKIEHDFD
                     WDAKQVIGSDRDTLVRLPLNRGLLDKSTYQLALQYDVAAGTKSMSYQVVDGDEVETYD
                     FRVLGEETVRTQAGLIDAIKVERVRDPTQSSRKTTLWFAKDWAFLLVRLHQVEKDGKE
                     YQIMLKEGTVDGKPVEGRRD"
     gene            complement(2100..2639)
                     /locus_tag="VFF22_11745"
     CDS             complement(2100..2639)
                     /locus_tag="VFF22_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HZX17523.1"
                     /translation="MALSLRDQLLKAGLVNEKQAKQAGKQQQKQQRLVHKGQAEKDES
                     QKLAAQQAMAEKAARDQELNRQQQEKIEQKARAAQVKQLIEASRLPKLTTEDYYNFVD
                     DKKVKRLSVNTLMRNKLSSGSLAIVRHGGGYEVIPREAALKIQERDPQRVVLLNTPTE
                     APDADDPYAAYQVPDDLMW"
     gene            complement(2711..3541)
                     /gene="mazG"
                     /locus_tag="VFF22_11750"
     CDS             complement(2711..3541)
                     /gene="mazG"
                     /locus_tag="VFF22_11750"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HZX17524.1"
                     /translation="MYQLDDLLHLMARLRDPQHGCPWDLKQNYATIVPHTIEEAYEVA
                     DAIERGDFDHLPGELGDLLFQVVYYSQLAREEGRFEFAQVVDCITTKLIRRHPHVFPD
                     GDLYGAVDVAKLEEAAVKQRWEEIKAEERAEKAAAPEQLSLLDDVPTALPSLSRAIKL
                     QKRAAQVGFDWPEALPVVDKVREELDEVLEAMSENDPEAISEEIGDLLFVVSNLARHL
                     KVDPETALRAANGKFELRFRFIEQALREAGRNMEDCALDELDALWGEAKKLEKQTPSC
                     "
     gene            complement(3569..5812)
                     /gene="relA"
                     /locus_tag="VFF22_11755"
     CDS             complement(3569..5812)
                     /gene="relA"
                     /locus_tag="VFF22_11755"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HZX17525.1"
                     /translation="MVQVRAHQPINDDGSINLEAWLGHVLSVDPALDREALREACEFA
                     REAEQQANAAKNLWTEGASSFRSGLEIAEILADLKLDQDSLVAAVIYRCVREGNVPLA
                     LVHQRFGPVVAKLVEGVLRMAAISASMNPRDSLVLGSQAQVENLRKMLVAMVDDVRVA
                     LIKLAERTCAIRAVKEADDEKRQRVAREVFDIYAPLAHRLGIGHIKWELEDLSFRYLE
                     PEQYKQIAKLLHERRLDREHYINEVMGQLRVELEATGVKADISGRAKHIYSIWRKMQR
                     KGLQFSQIYDVRAVRVLVPEVRDCYTALGIVHTLWRHIPKEFDDYIANPKENGYRSLH
                     TAVLGPEGKVLEVQIRTQAMHEEAELGVCAHWRYKGTDVKASSNHYEEKISWLRQVLE
                     WHEELGDIGGLAEQLRVDIEPDRVYVFTPDGHAIDLPKGATPLDFAYRVHTEIGHNCR
                     GAKINGRIVPLNYSLQTGEQVEIITSKHGTPSRDWLNSNLGYVTTSRARAKIVHWFKL
                     QDRDQNVAAGKVQLERELQRLALPHVDFDKLAEKANMKTSEDLFASLGAGDLRLAQLV
                     NLAQQLVEPDRVNEQLELIPRKALGFKPGKRGDIQIQGVGNLLTQMAGCCQPLPGDPI
                     VGYITQGRGVSIHRQDCASVLQLAGREPERIIQVNWGPVPVQTYPVEIIIKAYDRSGL
                     LRDVTQVLLNEKLNVLAVNTRSNKDDNTASMSITVEIPGLDALGRLLGRISQLPNIIE
                     ARRHRVS"
BASE COUNT         1123 a         1863 c         1730 g         1163 t
ORIGIN      
        1 ggcgtggtgg aaaaagccga aatcatcgac ggttcgaaag tcgccactgg tgacgcgctg
       61 atcgccctgc cgtcctccgg cccgcactcc aacggctact cgctgatccg caagatcatc
      121 gaagtgtccg gtactgaaat cgaaaccacc caggtcgacg gcaagccgct gaccgacctg
      181 ctgatggcgc caacccgcat ttacgtcaaa ccgctgctca aactgatcaa ggacactggc
      241 gcagtcaaag ccatggccca catcaccggt ggcggcctgc tcgacaacat cccgcgcgtg
      301 ctgccaaaag gcgcacaagc cgtggtcgac gtcgccagct ggacgcgccc agcggtattc
      361 gactggctgc aggaaaaagg caacgttgac gagcacgaga tgcaccgcgt gctcaactgc
      421 ggcgtcggca tggttatctg cgtggcgcag gatcaagtcg aagtcaccct gcagactctg
      481 accgctgctg gcgagcagcc ttgggtgatc ggtcagatcg gtgaagctgc cgaaggtgct
      541 gctcaggtcg tgctgaacaa cctgaaacag cactaatgtc ggcaccaatg ccaacagact
      601 gcaatgtggt ggtgctgatc tccggctctg gcagcaacct gcaggcgctg attgacagca
      661 tcgtccatga cagcaatccg gcacgcatcg ccgcggtgat ttccaaccgc gtcgatgcct
      721 acggcctgga gcgcgccaaa caggccggca tcgacaccgc cgtgctcgat cacaagcaat
      781 tcgacggccg tgaagccttc gatgccgcgc tggtcgaggc tattgatgcg tttaatccgc
      841 aactggtggt actggccggg tttatgcgca ttctcacccc cggtttcgtt cgtcactacg
      901 ccggccgcct gctgaatatc cacccttcgc tgctgccgaa atacaaaggc ctgcacaccc
      961 atcaacgggc gctggacgcc ggtgacagcg agcatggctg cagcgtgcac ttcgtcagcg
     1021 aggaactcga tggcggacca ctggtcgtac aggcagtggt accggtacag tcggatgact
     1081 cgccgaccag cctggcccag cgcgtacacg agcaagaaca tgtgatatac ccgctggccg
     1141 tgcgctggtt tgccgaaggc cgtttgcagc tgacgcagca aggggccatg ttggacgagc
     1201 aactgttacc gggtaccgga caccttattc gaacttagga gacaccatgg gtcgcgcctt
     1261 actgttcgca ctaacgcttc tgagcttgcc ggcattggcc gttgagctga aacccttctc
     1321 tgccagctac accgccgatt ggaagcaact accggtcagc ggcagcgccg aacgcagcct
     1381 gaaaaccctg gataacggcc tgtggcagct caacttcgaa gcctccatgc tggtcgccag
     1441 cctgaccgaa caaagcaccc tgcgcgtgga taaagaagta tttctgccgc agacctacct
     1501 gtacgagcgc aacggcctgg gtaagggcaa gaagatcgag catgacttcg actgggacgc
     1561 caaacaggtg atcggcagcg accgcgacac actggtgcgc ctaccgctga accgtggtct
     1621 gttggacaag tcgacctacc agttggccct gcagtacgac gtagccgccg gcaccaagag
     1681 catgagctat caggtggtcg atggcgatga agtggaaacc tatgacttcc gtgtgctggg
     1741 cgaagaaacc gtacgcaccc aggcaggcct gatcgacgcg atcaaggtcg agcgcgtgcg
     1801 tgatccgacc caaagcagcc gtaagaccac cctttggttt gccaaggatt gggcgttcct
     1861 gctggtgcgc ctgcaccagg tcgagaaaga cggcaaggaa taccagatca tgcttaagga
     1921 aggcacagtt gatggtaagc cggtcgaagg ccgccgcgac tgagcgcaca ccgggtacaa
     1981 aaaaccgagc cagtggctcg gtttttttat ggccggtgat gcggagagag gcagtgtgta
     2041 gcctggataa gcgtcagcgc aatccgggaa accaatgggt gcttaagcgg cgtccgccgt
     2101 caccacatca gatcatccgg cacctgataa gccgcgtacg gatcatctgc atccggggct
     2161 tcggtcgggg tgttgagcag caccacgcgt tgtgggtcgc gctcctgaat cttcagcgca
     2221 gcttcgcgcg ggatcacttc gtagccgccg ccgtggcgca cgatggccag cgagccgctg
     2281 ctgagcttgt tgcgcatcag ggtgttcacc gacaggcgct tgaccttctt gtcatcgacg
     2341 aagttgtagt aatcctcggt ggtcagcttg ggcaggcgcg aggcctcaat cagctgcttg
     2401 acctgcgcgg cacgggcctt ttgctcgatc ttttcctgct gttgacggtt caactcctgg
     2461 tcgcgcgcgg ccttctcggc catggcctgc tgagcggcga gcttttgcga ttcgtccttc
     2521 tcggcctgcc ccttatgcac caggcgctgc tgtttctgct gctgcttgcc ggcctgcttg
     2581 gcctgctttt cgttgaccag cccggcttta agcaactgat cgcgtagtga gagagccata
     2641 acctaccttt ccaaccatca atttaaagcg ggcgtgccca ccatcagcgg gcagaccgca
     2701 cccgcacaac tcaacaactt ggggtttgct tttccagctt tttggcttcg ccccagagcg
     2761 cgtccaattc atccagggcg caatcttcca tgttgcgccc tgcttcgcgc aatgcctgct
     2821 cgataaaacg aaaacgcagc tcgaacttgc cattggccgc acgcagggcg gtttccgggt
     2881 cgaccttcag atgccgcgcc aggttactga ccacaaacag caggtcgccg atctcctcgc
     2941 taatcgcctc ggggtcgttt tcgctcatgg cttcgagcac ttcatccagc tcttcacgca
     3001 ccttgtcgac caccggcagc gcctcgggcc agtcgaaacc aacctgggcg gcgcgtttct
     3061 gcagtttgat cgcccggctc agtgacggca gcgccgtggg cacatcgtcg agcaatgaga
     3121 gttgctcagg agcggcggcc ttttcggcgc gctcctcggc cttgatctct tcccagcgct
     3181 gtttaaccgc ggcttcttcc agcttggcca cgtccactgc gccatacaga tcgccatcgg
     3241 ggaacacatg gggatggcgg cgaatcaact tggtggtgat gcaatcgacc acctgggcaa
     3301 actcgaagcg cccttcctcg cgggccagct ggctgtaata caccacctga aacagtaggt
     3361 cgccaagctc acccggcaga tgatcgaaat cgccgcgttc gatggcatca gcaacctcgt
     3421 acgcttcttc gatggtgtgc ggcacgatcg tcgcgtagtt ctgcttgagg tcccacgggc
     3481 agccgtgctg cgggtcgcgc agccgggcca tcaggtgcag caggtcgtcg agttggtaca
     3541 tggcaaatcc agttaagtcg taatcgcctc aggaaactcg atgacggcgc gcttcgatga
     3601 tattcggcag ctgcgaaata cgccccaaca aacgcccaag cgcatccagc cccggaatct
     3661 ccactgtaat agacatggac gcggtattgt cgtccttatt cgagcgggta ttcaccgcca
     3721 gtacattgag cttctcgttg agcagcacct gggtcacgtc acgcagcagg ccggaacggt
     3781 cgtaggcctt gatgatgatt tccaccgggt aggtctgcac cggcaccggt ccccagttga
     3841 cctggataat ccgctccggc tcacgcccgg ccagctgcaa caccgaggcg cagtcctgac
     3901 ggtggatgct gacgccacgg ccctgagtga tatagccgac aatcggatcg cccggcagcg
     3961 gctggcagca accagccatc tgcgtcagca ggttgccgac gccctgaatc tggatatcgc
     4021 cgcgtttgcc cggcttgaag cccagcgcct tgcgcgggat cagctccagc tgctcgttga
     4081 cccgatccgg ctcgactagt tgctgcgcca ggttgaccag ttgcgccagg cgcaggtcac
     4141 ccgcgccaag ggaggcgaac agatcctcgc tggtcttcat attggccttc tcggccaact
     4201 tgtcgaagtc cacatgcggc agcgccagac gctgcagttc gcgctccagc tggaccttac
     4261 cggccgcgac gttctggtcg cggtcctgca gtttgaacca gtggacgatc tttgcccgcg
     4321 cccggctggt ggtgacgtaa ccgaggttgg agttcagcca gtcgcggctc ggcgtaccgt
     4381 gcttgctggt gatgatctcc acctgctcgc cggtctgcag gctgtagttg agcggcacga
     4441 tccgcccatt gatcttcgcg ccacggcagt tgtggccgat ctcggtgtgc acgcgatagg
     4501 caaaatccag cggcgtcgcg cccttgggca ggtcgatggc gtgaccgtcc ggggtgaaga
     4561 catagacccg gtccggttcg atatccacgc gcaactgctc agccaagccg ccgatatcac
     4621 ccaattcctc gtgccattca agcacctggc gcagccagga aatcttctct tcgtaatggt
     4681 tggagctggc tttgacgtcg gtgcccttgt agcgccagtg cgcgcacacg cccagctcgg
     4741 cttcctcgtg catggcctgg gtgcggatct gcacttccag caccttgcct tccgggccca
     4801 gcacagcggt gtgcagtgag cggtagccgt tctccttggg gttggcgatg tagtcgtcaa
     4861 actccttggg aatatgccgc cacagggtgt gcacgatacc gagggcggtg tagcaatcgc
     4921 gcacctccgg caccagcaca cgcaccgcgc gcacgtcgta gatctggctg aactgcaggc
     4981 ccttgcgctg cattttgcgc cagatcgaat agatgtgttt ggcccgcccg ctgatgtcgg
     5041 ccttgacccc ggtggcctcc agctccacgc gcagctgacc catcacttca ttgatgtagt
     5101 gctcgcggtc taaacgacgc tcatgcagca gcttggcgat ctgcttgtac tgctcgggct
     5161 ccaggtagcg gaacgacagg tcttccagct cccacttgat atggccgatg cccaggcgat
     5221 gggccaacgg tgcgtagatg tcgaacacct cgcgggccac gcgctgacgc ttctcgtcat
     5281 cggcctcctt caccgcgcgg atcgcgcagg tgcgctcggc cagtttgatc agcgccacgc
     5341 gcacgtcatc gaccatggcc acaagcatct tgcgcaggtt ttccacctgc gcctgggagc
     5401 caagcaccag agagtcgcgc gggttcatcg aagcgctgat cgccgccata cgtagcacgc
     5461 cttcgaccag cttggccacc accgggccga aacgctgatg caccagcgcc aacggcacat
     5521 tgccttcgcg cacgcagcgg tagattacgg cggcgaccag ggagtcctga tcgagcttga
     5581 ggtcggcgag aatttcggcg atttccagac cggagcggaa actagacgcg ccctcagtcc
     5641 atagattctt cgcggcattg gcctgctgct cggcctcccg ggcgaactcg caggcctcgc
     5701 gcagggcctc gcgatccagt gccggatcga cactcagtac atgcccgagc caggcttcaa
     5761 ggttgatgct gccgtcatcg ttgatcggct gatgcgctct gacctgaacc atcttgctta
     5821 ccttccctac ggtgcgcggc atgcacaccg aacagtcgcc gccttctatg agccggtcg
//
LOCUS       DASRUM010000183         6267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_613731, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000183 DASRUM010000000
VERSION     DASRUM010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613731"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1161)
                     /locus_tag="VFF22_11760"
     CDS             complement(25..1161)
                     /locus_tag="VFF22_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HZX17526.1"
                     /translation="MDKLLKLWQSTGLYHVEPGQLFMMAVCILLIYLAIKKGFEPLLL
                     LPIGFGGLLANIPVANMAEGAGFLHMIYEVGLPTSIFPLLIFLGVGAMTDFGPMLANP
                     KTLLLGAAAQFGIFGTLMGALALTALGIPGLEFTLKEAASIAIIGGADGPTSIFVTSK
                     LAPHLLGPIAVAAYAYMALVPLIQPPIMRALTTKEERAIVMQQLRPVGQAEKIIFPIV
                     LCLLIGMLLPDAAPLIGMFALGNLLREAGVVERLADTSRNALINIVTIFLGLTVGSKL
                     SADSFLQLKTLGILSLGMLAFCAGTAAGVLMAKLMNMFSSNKINPLIGSAGVSAVPMA
                     ARVSNKVGLEANPQNFLLMHAMGPNVAGVIGSAVAAGVLLSFVG"
     gene            complement(1174..2946)
                     /gene="oadA"
                     /locus_tag="VFF22_11765"
     CDS             complement(1174..2946)
                     /gene="oadA"
                     /locus_tag="VFF22_11765"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HZX17527.1"
                     /translation="MTAVKKALGITDVVLRDAHQSILATRVRLEDMLPIAPKLDQVGF
                     WSVESWGGATFDACIRYLGEDPWHRIRELKKAMPNTRQQMLLRGQNLLGYRHYADDVV
                     EKFVERAAFNGVDVFRVFDAMNDPRNLQTALKAVKQLGKHAQGTISYTTSPVHTLEMW
                     VDLAKQIEDMGADSVAIKDMAGILTPYTAFELVTRLKATLSIPIHMQCHATAGLSSVA
                     ILKAVEAGIDNVDTAISSLSMTYGHSPTESVVAMFQGTERDTGLDLLLLEEIAAYFRE
                     VRKKYAKFEGNLKGVDSRILVAQVPGGMLTNMEGQLKEQGAQDKFDEVLAEIPRVRED
                     LGFIPLVTPTSQIVGTQAVINVLTGERYKSITKETAGILKGEYGSAPAPFNKELQARV
                     LDGAEAVTCRPADLLEAEMDKLTVELKGIAKEKGIKLAADEIDDVLTYALFPQIGLKF
                     LENRGNPAAFEPAPTGKDAPAREAGKPEVYTVEVGGKSYVVQVNEGGDIEGLKAVGGA
                     NSSAAVAAPVGAGEPQNAPLAGNIFKVLVQPGHAVEEGQLVIILEAMKMETEIRAFKA
                     GTVGAVNVKVGDAVAVGDSLLTIG"
     gene            complement(2972..3223)
                     /locus_tag="VFF22_11770"
     CDS             complement(2972..3223)
                     /locus_tag="VFF22_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HZX17528.1"
                     /translation="MTPSELLLEGVELMLFGLGTVFLFLMLLVLMIRVMSWVIERFTV
                     TVPIPVSVPSAVKPASDEPSADVLAAIQSAIHQHRARRG"
     gene            complement(3399..4841)
                     /gene="mgtE"
                     /locus_tag="VFF22_11775"
     CDS             complement(3399..4841)
                     /gene="mgtE"
                     /locus_tag="VFF22_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HZX17529.1"
                     /translation="MTEVEAKKPQESLQDRLAQVVELLHRHKVVEDLTHRQEGQHHSR
                     VENLVHRQNLVELQRKLDDLHSADVAHILEALPLDDRLTIWQLVKVERDGDILLEVSD
                     AVRETLLADMDDHEILAAARDMDADELADLASELPRDVVHELMETLDAQQRERVRSAL
                     SYEDDQVGALMDFEMVTIREDVSLEVVLRYLRRLKELPGHTDKLFVVDYDGVLKGVLP
                     VKRLLVNDPEKLVSEVMATDPVSFHPDEDGYDAAQAFERYDLISTPVVDKNGKLIGRL
                     TIDEMVDLIREESESEVLNMAGLREEEDIFASVWKSLRNRWAWLAINLVTAFMASRVI
                     GLFEDSIEKLVALAALMPIVAGIGGNSGNQTITMIVRAMALDQVSTGNTSRLIRKELG
                     VGLVNGIVWGGVIGVVAYLLYDSWSLGVVMTAAMILNLLLAALMGVLIPMTLVRMGRD
                     PALGASVMITAVTDSGGFFIFLGLASLFLL"
     gene            5164..5661
                     /locus_tag="VFF22_11780"
     CDS             5164..5661
                     /locus_tag="VFF22_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020290521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HZX17530.1"
                     /translation="MHGLHRAVFCAILLCPLPTLSSTVFRCEDAKGHVTFTRQGCPDA
                     HNQSLQQAYNPTPGSGKPVPLAKPRKASRQSKKKDEPELTVIGVKQDGCGNHLNSSER
                     RAAIIKQQIRLGMSRSDVESSLGKPDKITEQNGQTRYVYQGKKGNKRNVSFDEAGCVK
                     GKAKR"
     gene            complement(5710..5786)
                     /locus_tag="VFF22_11785"
     tRNA            complement(5710..5786)
                     /locus_tag="VFF22_11785"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5750..5752),aa:Arg,seq:acg)
     gene            complement(5859..5935)
                     /locus_tag="VFF22_11790"
     tRNA            complement(5859..5935)
                     /locus_tag="VFF22_11790"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5899..5901),aa:Arg,seq:acg)
     gene            complement(6008..6084)
                     /locus_tag="VFF22_11795"
     tRNA            complement(6008..6084)
                     /locus_tag="VFF22_11795"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6048..6050),aa:Arg,seq:acg)
     gene            complement(6137..6227)
                     /locus_tag="VFF22_11800"
     tRNA            complement(6137..6227)
                     /locus_tag="VFF22_11800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6191..6193),aa:Ser,seq:gct)
BASE COUNT         1390 a         1938 c         1736 g         1203 t
ORIGIN      
        1 cgtttttttt attgctcgta ttaatcagcc aacaaaactg agcaatacac ccgcagccac
       61 tgccgagccg atcaccccgg ccacgttcgg gcccatggcg tgcatcagca ggaagttctg
      121 cgggttggct tccagcccaa ccttgttcga cacccgcgcc gccatcggca ccgcagagac
      181 gccggccgag ccaatcagcg ggttgatctt gttgctgctg aacatattca tcagcttggc
      241 catcaacacc cccgcagcag taccggcgca gaacgccagc atgcccaaac tgaggatgcc
      301 cagggttttc agctgcagga aggaatccgc cgacaacttc gaacccacgg tcagaccgag
      361 gaagatggtt acgatattga tcagcgcgtt gcgcgaagta tcggccaggc gctcgaccac
      421 accggcctca cgcagcaggt taccaagagc gaacatgcct atcagcggcg cagcatcggg
      481 cagcagcatg ccgatcagca ggcacagcac gatcgggaag atgatcttct cagcctgccc
      541 aacagggcgc agctgctgca taacgatggc gcgctcttcc ttggtggtca gcgcgcgcat
      601 gatcggcggc tgaatcaacg gcaccagcgc catataggca taggccgcca cggcaatcgg
      661 accgagtaga tgcggagcca gcttggaggt cacgaaaatc gaggttgggc cgtcggcacc
      721 gccgatgatg gcgatggaag cagcctcctt gagagtgaac tcaagccccg gaatacccaa
      781 cgccgtcagc gccagtgcac ccatcagcgt gccgaaaatc ccgaactgcg ccgcggcccc
      841 gagtaacagg gtcttggggt tggccagcat cggcccgaag tcggtcatgg cgccgacgcc
      901 gaggaatatc agcagcggga agatactggt gggcaggccc acctcgtaga tcatgtgcag
      961 gaaacccgcg ccctcggcca tattggctac cgggatgttg gccagcagac cgccaaaacc
     1021 aatcggcaac agcagcagcg gctcgaagcc cttcttgatc gccagataga tcagcaggat
     1081 gcacaccgcc atcataaaca gctgacccgg ctcgacgtgg tacaggccgg tgctctgcca
     1141 aagcttgagg agcttatcca ttaccgcgct cccttaaccg atggtcagca ggctgtcgcc
     1201 caccgccacc gcatcaccta ctttgacgtt gaccgcaccc acggtgccgg ccttgaacgc
     1261 acggatctcg gtttccatct tcatcgcttc gaggatgatc accagctgcc cttcttccac
     1321 ggcatgacct ggctgcacca gtaccttgaa gatattgccg gccagcgggg cattctgcgg
     1381 ctcgccggcg cccactggcg cagctactgc tgccgaggaa ttagcaccac cgacagcctt
     1441 gagcccttcg atatcgccgc cttcgttgac ctgcaccaca taggacttgc cgcccacttc
     1501 aacggtgtag acctcaggtt tgccggcttc acgagcaggt gcgtctttgc cggtcggtgc
     1561 cggctcgaag gctgctgggt tgccgcgatt ttccagaaac ttcagaccaa tctgcgggaa
     1621 cagggcgtag gtcagcacgt cgtcgatttc gtcagcggcg agcttgatgc ccttctcttt
     1681 ggcaatgccc ttgagctcga cagtcagctt gtccatctcg gcttcaagca gatcggccgg
     1741 acggcaggta accgcttcag cgccatccag aactcgtgct tgcagctctt tattgaaagg
     1801 tgccggcgct gaaccgtatt cacccttcaa gatgccggcg gtttccttag tgatcgactt
     1861 gtaacgctca ccggtcagta cgttgatcac cgcctgggtg ccaacgatct gcgaggtcgg
     1921 ggttaccagc gggataaagc cgaggtcttc acgcacgcgc ggaatctcag ccagcacttc
     1981 gtcgaatttg tcctgggcgc cctgctcttt cagctggcct tccatattgg tcagcatgcc
     2041 gcccggcacc tgagcaacca gaatgcgcga gtcgacgccc ttgaggttgc cctcaaattt
     2101 cgcgtacttc ttgcgtactt cacggaagta ggcggcgatc tcttcgagca gcaacagatc
     2161 cagaccagta tcgcgctcag tgccctggaa catcgccact acggattcgg ttggcgaatg
     2221 gccgtaggtc atcgacaacg aggaaatggc ggtatcgaca ttgtcgatgc ctgcttccac
     2281 ggccttaaga atggccacag acgacagccc agcagtagcg tgacactgca tatgaatcgg
     2341 gatcgacagg gttgccttca ggcgcgtcac cagttcgaag gcggtgtaag gcgtcagaat
     2401 accggccatg tccttgatgg ctaccgagtc ggcgcccatg tcttcgatct gcttggccag
     2461 gtcgacccac atttccagag tatgcacagg gctggtggtg taggagatgg tgccctgggc
     2521 atgcttgccc agctgcttga cggccttgag cgcggtctgc aggttgcgtg gatcgttcat
     2581 cgcgtcaaat acgcggaaca catcaacacc attgaaggcg gcgcgctcga cgaatttttc
     2641 caccacgtca tcggcgtaat ggcggtagcc cagcaggttc tgcccgcgca gcagcatttg
     2701 ctgacgggtg ttgggcatgg cctttttcag ctcgcgaatg cggtgccatg ggtcttcacc
     2761 caagtaacgg atacacgcat cgaaggtcgc gcccccccag gattccacgg accagaagcc
     2821 gacctgatcg agcttgggcg cgatcggcag catgtcttcg aggcgcacgc gggtggccag
     2881 gatcgactgg tgggcgtcac gcagcaccac atcagtaatt ccgagtgctt tctttacggc
     2941 agtcatacag atgctccctg tcgaacctgg ctcacccgcg gcgggcgcgg tgttggtgaa
     3001 tagcggattg gatagcggct agcacatcag cgctcggctc gtcggaggcc ggcttgactg
     3061 ctgatggcac agatacggga attggcaccg tcacggtaaa acgctcgata acccacgaca
     3121 tcacgcggat catcagcacc aatagcatca ggaagagaaa tacggtgccc aggccgaaca
     3181 gcatgagctc gacaccctca aggaggagtt cactaggggt catcttggct ccattaccag
     3241 ctacgtgtga gctgcgttaa ttattagata ggagcgcccg ggaactgatc gccgggcaaa
     3301 gctggcgaac cttagcgtga aacgagcgtt tgaagcaaac ccagagcagc ctaatgagac
     3361 attttctgta gagaaagcgt caccactcca gcaggccgct aaagcaggaa cagactggcc
     3421 aagcccaaga agataaagaa gccgccgcta tcggttaccg cggtgatcat cacactggca
     3481 cccaaagctg gatcacgacc catacgcacc agcgtcatgg ggatcagcac ccccatcagc
     3541 gctgccagca acaggttcag gatcatcgca gcagtcatca ccacacccag cgaccagctg
     3601 tcatagagca ggtaggccac cacgccaatc accccacccc agacgatacc gttgaccaac
     3661 cccacaccca actccttgcg aatcaggcgc gaggtattgc cggtgctgac ctgatccagc
     3721 gccatggcgc gcacgatcat ggtgatggtc tggttacccg agttgccacc gatgcccgca
     3781 acaatcggca tcagcgccgc cagggcgacc agcttctcga tggaatcttc gaacaggccg
     3841 atcaccctcg acgccataaa ggcagtaacc aggttgatcg ccagccaggc ccaacggttg
     3901 cgcagggatt tccacaccga ggcgaagatg tcttcttcct cgcgcagacc ggccatattg
     3961 aggacttcgc tttcgctttc ttcacggatc aggtcaacca tctcatcgat ggtcaaacgg
     4021 ccaatcaact tgccgttctt gtccaccacc ggcgtagaga tcaggtcata acgctcgaac
     4081 gcctgagcgg cgtcgtagcc gtcctcatcc gggtggaaac tcaccgggtc ggtggccatc
     4141 acttcgctga ccagtttctc tgggtcattg accagcagac gcttgaccgg cagcacgccc
     4201 ttgagcacgc catcgtaatc gaccacaaac agcttatctg tgtgccctgg caactccttg
     4261 aggcggcgta gatagcgcaa caccacttcc aggctgacat cttcgcggat agtgaccatc
     4321 tcgaagtcca tcagcgcgcc gacctgatcg tcctcgtagg acaacgccga gcgcacgcgc
     4381 tcgcgctgct gcgcatcgag ggtttccatc agctcatgca caacatcgcg cggcaattcg
     4441 gaggccaggt cggccagctc gtcggcgtcc atgtccctgg cggcagcgag gatttcatgg
     4501 tcgtccatgt ccgcgagcag ggtttcacgc actgcgtcgg acacttcgag gaggatgtcg
     4561 ccgtcgcgct cgaccttgac cagctgccag atagtcaggc ggtcatcaag cggcaaggct
     4621 tcgagaatgt gggcaacgtc ggcggagtgc agatcgtcga gtttgcgctg caactcgacg
     4681 agattctgcc ggtgaaccaa gttttccacc cggctgtggt gctgcccctc ctgacggtgg
     4741 gtcaagtctt cgactacttt gtgccgatgc agcagttcaa ccacctgggc caggcggtcc
     4801 tgcaggcttt cctgcggttt ttttgcttct acttcagtca tggcgcgctc cacccccagc
     4861 ggcagcgcac gccaaagagg gtcaatcagt caacacgtga ttgcacaatc gagattcaga
     4921 gtaactactg ggtaagtcca tggtggtttt ccacaagccc cggcggggct gacgcggcga
     4981 atgataccac cgcccaggcg ctttgcatgt gacaaaaaca atcctagaac aagcgcttgc
     5041 acgacaaact taagcgcgcc cgcacatctg taattgcgac gtgccgggca ttggcgaagg
     5101 cacgcctggc gcagacaaaa gcatgagcta cccttgcaca tggaccgtca cggaggacgc
     5161 accatgcatg gactgcaccg cgcagttttc tgcgccattc tgctttgccc actgcctacg
     5221 ctcagctcaa ccgtatttcg ctgcgaagac gccaaaggcc acgtcacctt cacccgccag
     5281 ggttgccccg acgcacacaa tcaaagcctg caacaggcct acaaccccac acccggcagc
     5341 ggtaagccag tgccattggc caaaccgcgc aaggccagcc gccagagcaa gaaaaaagat
     5401 gagcctgaac tgacagtaat cggcgtcaag caggacggtt gtggcaacca cctgaatagc
     5461 agtgaacggc gcgccgctat catcaagcag cagatacgcc tgggcatgag ccgcagcgat
     5521 gtcgagagca gcctcggcaa gcctgacaag atcaccgaac agaacggcca gacgcgctac
     5581 gtttaccagg gtaagaaagg taacaagcgc aacgtcagct tcgatgaagc cggctgcgtc
     5641 aaaggcaagg ccaaacgctg aattcggcga ataaaaaaag ggcctgcatc gctgcaggcc
     5701 cttcttgttt ggtgcactca ggaggattcg aacctccgac cgctcggttc gtagccgagt
     5761 actctatcca gctgagctat gagtgcaatt ggcgcttttt aaccagatca ccactggctg
     5821 aaacaaaatg gcctgcatcg ctgcaagcca ttccgatttg gtgcactcag gaggattcga
     5881 acctccgacc gctcggttcg tagccgagta ctctatccag ctgagctatg agtgcaaatg
     5941 gcgcttttta accagatcac cgctggctga aacaaaatgg cctgcatcgc tgcaagccat
     6001 tccgatttgg tgcactcagg aggattcgaa cctccgaccg ctcggttcgt agccgagtac
     6061 tctatccagc tgagctatga gtgcatgtaa cgcttgcact gtagacttct aaagcaacgc
     6121 ctacagcact aaataatggc ggagaagggg ggattcgaac ccccgacacc cttttgagat
     6181 gtactccctt agcaggggag cgccttcggc cactcggcca cctctccgca acacggggcg
     6241 catgataacc atgttttccc cgtttgc
//
LOCUS       DASRUM010000184        11095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1923533, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000184 DASRUM010000000
VERSION     DASRUM010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 11095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11095
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923533"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="VFF22_11805"
     CDS             complement(<1..1135)
                     /locus_tag="VFF22_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17531.1"
                     /translation="MNRHLREILFILLALPGLSMAMEALDDRELGEVSGAGVGFFLDD
                     FFYDQGSASAQVSGLKDSQGNALTIDLERAYIKGDGSQRGALDTPAQVGTPLHPFTLK
                     VVSGAAAPTLPSGTQALQLTTPTWDDPLNDTHQYGLWSYYQGCLYGEAGCTNPTQAQV
                     AIDTELSSLETTRNALLARYQSVGFLTLKSGIDQDMLLVEQRRTQVSTETTDVQATYG
                     TMQTRYAAAPATADLFYAKPAFGQKYGCGNICINAAARAYNTSVDSYQTQLSELAAAQ
                     KALGEAWSVQRHGYSLNQRATDFDEFSSLCGAPTAESPTCVVGRIQKTKDNRNSVVLV
                     ATSLQNGGTRVKGLDVGVEATFSLPSTAYSVDGNGNIIKGATTT"
     gene            complement(1138..2244)
                     /locus_tag="VFF22_11810"
     CDS             complement(1138..2244)
                     /locus_tag="VFF22_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017516271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6160 family protein"
                     /protein_id="HZX17532.1"
                     /translation="MRPTCHPLKPLLTLLCGSLFVCAAQAELRPMADNELAEITGQAL
                     ITLDGLTYGGFEYTRVNLGAEIDLLTNIDELRLGNYSRNQPNTASTQPADIRIDNFAL
                     GRVDNANSSNATVVPFQVRDPYLEFAFKVNGQGIREVAGIRLGFGRARGDLSGDIHSL
                     TGNMQGLVTGSASTAREYYMQQNNLNGFTCTFNFDCLALSLAGNAEIYSEIQLVKNGT
                     GDTTVNGQPINRATYIGVPNGSSFNTDNALLSGLLPSIAKNNGDCTASGLPACFPLLN
                     YKSIFVGDRTQSDLSIGGAQGIFFSLQNQNVPWQDLASTNNQFVATQSGAFANFAKSG
                     EGANTIYPFIVSLYEALNGAARVPTCIGDLSKGC"
     gene            2516..3763
                     /gene="mksB"
                     /locus_tag="VFF22_11815"
     CDS             2516..3763
                     /gene="mksB"
                     /locus_tag="VFF22_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mks condensin complex protein MksB"
                     /protein_id="HZX17533.1"
                     /translation="MIDPKRVLRALAEHWTLLEPLCERFDAGTLSLVELRNQLAAQLP
                     EGTPTDVTALLDQWIRLDILVPVAKSPNRFELNAQIHDFLAYLRREHRLGLCLEIEAY
                     LRHLERLAGYIKDAFEVRDAADLARQLRLLDMRVRDVLKKLANDEQALVAVADRAKTS
                     DRQIPLRQRYAEVLATWDEYVEPMIQLVAADGAFEQGVYRVEHVLLRLLGEQQRLGQL
                     VDDDLLLRTHARILEMQTTAQLTLRRARELLLPLREEARRHNAVTRGAALALAAIRKK
                     GLDAVPQASLPLFSRPQSTFLGTASQVEAYVYALARFEPKPAQFPKAHASRKGEAPRA
                     PRTAREMIERCEQALPLPDLMVWLLEQEPEGATDELLYWFSRLSRDGRFQRERLERRE
                     YLTREHQVSLSSFALIKPAGTTV"
     gene            3760..4455
                     /gene="mksE"
                     /locus_tag="VFF22_11820"
     CDS             3760..4455
                     /gene="mksE"
                     /locus_tag="VFF22_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mks condensin complex protein MksE"
                     /protein_id="HZX17534.1"
                     /translation="MNIDLKELTQLAPIFRELFKGYHISRSNPELYSQLASQQDQYRG
                     LFKALGYELTCDSRGFYYFVPEQMGTQVNKTAQRLALFTFILVEHLADQGRDPLAVLD
                     GGSLGRDELPALLEKYRDLFLQAEVTTQDELEEKVMRRLTQLGFASEDNGIYRFLAPM
                     HRFLDVCLSVQQDRDLAATLHSSLPLPTPVLLDEETEDEIISLAEGDAEESEEDALAR
                     AMAEERDAKETLA"
     gene            4452..7286
                     /gene="mksF"
                     /locus_tag="VFF22_11825"
                     /pseudo
     CDS             4452..7286
                     /gene="mksF"
                     /locus_tag="VFF22_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362518.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mks condensin complex protein MksF"
     gene            complement(7339..9642)
                     /locus_tag="VFF22_11830"
     CDS             complement(7339..9642)
                     /locus_tag="VFF22_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZX17535.1"
                     /translation="MNDLPLAKARPASNWSAIWVLPLIALLIGGWLGWRAYNEAGIEI
                     QVMFASGDGIQPGKTEVIFKGMPIGKVIALELDDSGERRGVRATLEMDKRVEQHLRSN
                     TRFWLVKPSVSLAGITGLETLVSGNYITASPGDGEPTRKFTALSEPPPLADTAPGLHL
                     TLKAERLGSLNRDSPVFYKQIQVGRVKSYALVEEQNLIEVKVFIEPEFASLVRKHTRF
                     WNASGVSIDAGLSGVKVRTESLASIVAGGIAFATPEHRKDSPPTDPTLPFRLYEDFDS
                     AQAGIRVSLKLHEFEGLEPGRTPVMYKGIQVGHMKTFKVDQDLSGARVELMLDPRTED
                     YLVEGTDFWVVKPSISLAGITGLEALVKGNYIAIRPGDAGNPPLRDFVARAKAPPLDL
                     SAPGLHLVLFSDTRGSLEVGSPILFKQVKVGTVQSFQLSRDDKQVAFGVHIEPEHAHL
                     VNSSTRFWNASGITLKGGLSGVEVKSESLQTLLAGGIAFETPNPNAPKGSRRVERFNL
                     HSSQELALQKGVELTIRVLSGDGLGPGTAIRYKGLEVGKVERIELTDDLQAVILYARI
                     TQATQQIARAGTQFWVVKPEVSLTRAANLETLVSGQYLEVRPASKPGAAQVSFTAAES
                     APTQNAREQGLRLVLSAARRGSIKPGVVVSYREVPVGKVTDFELGPTSDRVLIHVLIE
                     PRYAPLVRSGSRFWNASGIGVDAGLFSGVKVRTESLEALLEGGIAFASPDNPQMGGPA
                     QAGQTFALHDEVSEEWLRWAPKIALEK"
     gene            complement(9635..10258)
                     /locus_tag="VFF22_11835"
     CDS             complement(9635..10258)
                     /locus_tag="VFF22_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008719782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HZX17536.1"
                     /translation="MRAIDAGLLICHECHQLNPIERGQGAQFCTRCGGHVHARRPNSL
                     VRTWALLLTAAVLYIPANLLPIMTVNSLGKGQSDTIMSGVIELVNYGMLPIAAVVFIA
                     SILVPTFKLVGIALLLYSVQRHQPMSARQRVLMYRFIEWIGRWSMLDIFVIAILVALV
                     NFGSLASIVPGYGATAFASVVVLTMLAALTFDPRLIWDNTDSDDHHE"
     gene            complement(10245..10904)
                     /locus_tag="VFF22_11840"
     CDS             complement(10245..10904)
                     /locus_tag="VFF22_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HZX17537.1"
                     /translation="MADPALSASLSELELKQLIACHECDLLLRKPQVAFGERVECPRC
                     GYELYTHRAQVVRRSMALVIAALLLYVPANFLPIMQLNLLGQATEDTVWTGVLGLYNS
                     GMQSVAVVVFLCSMAVPLLKLLCQFAVLLSIRLDIGRSYGLLLYRIYHHMREWGMLEV
                     YLMGILVAIVKLIDLADLSIGLGLLCFIALLLVQVWLEVTMSPHQIWEALSGEDTHAG
                     D"
BASE COUNT         2398 a         3548 c         3146 g         2003 t
ORIGIN      
        1 gggtggtggt ggcacctttg atgatattgc cgttgccgtc cacgctgtag gcagtgctgg
       61 gcaggctgaa ggtggcttcg acaccaacat ccaggccctt gacgcgggta ccgccgtttt
      121 gcaggctggt cgccaccaga accaccgagt tgcggttgtc cttggttttc tgaatacgcc
      181 cgaccacgca ggtaggcgac tcggcggtgg gcgcgccgca taggctggaa aactcgtcga
      241 aatcggtggc ccgctggtta aggctgtagc cgtgacgctg gacactccag gcctcaccca
      301 aggccttctg agcagcggcc agctcactaa gctgggtctg atagctatcg accgaggtgt
      361 tgtaggcacg cgccgccgcg ttgatgcaga tattgccgca gccgtatttc tggccaaatg
      421 ctggcttcgc atagaacagg tcggcggttg ccggtgccgc ggcataacgg gtttgcatgg
      481 tgccgtaggt agcttgtaca tcggtggttt ccgtggacac ctgggtacgg cgctgctcga
      541 ccagcaacat atcctggtcg ataccgctct tcagagtaag gaaaccgacg ctctgatagc
      601 gcgccagaag cgcattgcgg gttgtttcca gactactcag ctcagtgtcg atggcaacct
      661 gagcctgggt tgggttggta cagccggcct cgccatataa acagccctga taataggacc
      721 acagaccata ctggtgggtg tcgttgagcg ggtcgtccca ggttggcgtg gtcagctgca
      781 gggcctgagt gccgctcggc agggtcggtg cggcagcacc actgactacc ttcagggtaa
      841 aggggtgtag cggcgtgccc acctgcgccg gggtgtccag cgcgccgcgc tgactgccat
      901 cacccttgat ataggcccgt tcgaggtcaa tggtcagagc attaccctgg ctgtccttaa
      961 gcccgcttac ctgcgcgctg gcactgccct ggtcgtagaa aaagtcgtcc agaaagaagc
     1021 caaccccggc cccgctgacc tcaccgagtt cgcgatcatc cagggcttcc atggccatac
     1081 tcagcccagg cagagccagc aagataaaca gtatttcgcg cagatggcgg ttcatgatca
     1141 gcagcccttg ctcaggtcac cgatacaggt cggtacgcgt gcggcaccgt tgagggcctc
     1201 atacagcgac acgataaagg gatagatagt gttagcgccc tcgcccgact tggcgaagtt
     1261 ggcaaaggcc ccggactggg tcgcaacgaa ctggttattc gtcgaggcca ggtcctgcca
     1321 gggtacgttc tggttctgca gagaaaagaa tatcccctgg gctccgccga tactgaggtc
     1381 ggactgggta cggtcgccga cgaaaatgct cttgtagttg agcagcggga agcaggcggg
     1441 cagccccgag gcggtgcagt cgccgttgtt cttggcaatc gacggcagca gaccactgag
     1501 caaggcgttg tcggtgttga aggacgagcc gttaggcacg ccgatataag tagcccggtt
     1561 gattggctga ccattgaccg tggtatcgcc ggtgccgttc ttgaccaatt gaatctcgct
     1621 gtaaatctcg gcattgccgg ccaggctcaa cgccaggcag tcgaaattga aggtgcaggt
     1681 aaagccgtta aggttgttct gctgcatata gtattcgcgg gcggtcgatg ccgaaccagt
     1741 caccagcccc tgcatattgc cggttagcga gtggatatcg ccggacaggt cgccccgcgc
     1801 gcggccaaac cccagacgga tacccgccac ctcgcggatg ccctggccgt tgaccttgaa
     1861 cgcaaactcc agatagggat cgcgtacctg gaacggcacc acggtggcat tgctggagtt
     1921 ggcattatcc actcgtccca gagcgaagtt atcaatgcgg atatctgccg gttgagtgga
     1981 cgcagtattt ggttggttgc gcgaatagtt gcccaggcgc aattcgtcga tgttggtcag
     2041 caggtcgatc tcggccccaa ggttgacccg ggtgtactca aaaccgccgt aggttaggcc
     2101 gtcgagggta atcaatgcct ggccggtgat ctcagcaagc tcgttgtccg ccatgggcct
     2161 caattcggct tgggccgcac atacgaacag gctgccgcag agcagggtca gtaaaggctt
     2221 gagcggatgg caggtcgggc gcatgacgag ctccttattc ttatgacgct ggagtcctgc
     2281 ggacaccagc ccttgattgc cactataaaa agcctgcgtc tagagcgggc cgacccatag
     2341 tcgagaaaac ccgggaaaat cgccctctgc ccaggcccgg cctgttaccc attgtcacac
     2401 cgacggttac aggcaacgta gcaacgcaac agacgatgac tgacactcaa gccttggccg
     2461 tggcttgcag tacaatcggc gttttttgcg aaaagcccgc ggagcggacg ccccgatgat
     2521 tgatcccaag cgcgtactgc gcgcccttgc cgaacactgg acgttgctgg agccgttgtg
     2581 cgagcgcttc gatgccggca ccctgagcct ggtggagctg cgcaatcagc tcgccgcaca
     2641 gttgcccgaa ggcacgccaa ccgatgtcac cgccctgctc gaccagtgga ttcgcctgga
     2701 tatcctggtg ccggtggcca agagcccaaa tcgcttcgag ctgaacgcgc agattcacga
     2761 cttcctcgcc tacctgcgcc gcgaacatcg cctgggcctg tgcctggaaa tcgaagctta
     2821 tctgcgccat ctggagcgcc tggccggcta tatcaaggac gccttcgagg tccgtgacgc
     2881 cgccgacctg gcgcgccagc tgcgcttgct cgatatgcgc gtgcgcgatg tattgaagaa
     2941 gctggccaac gacgaacagg ccctggtcgc cgtggccgat cgggccaaga cctcagatag
     3001 gcagattccc ctgcgtcaac gctacgccga ggtgctggcc acctgggacg aatacgtcga
     3061 gccgatgatc cagctggtcg ctgctgacgg cgccttcgaa caaggtgtct accgcgtcga
     3121 acacgtgctg ctgcgcctgc tcggcgagca gcagcgtctg ggccaactgg tcgacgacga
     3181 cctgctgctg cgcacccatg cgcgcattct ggaaatgcag accaccgccc agctgaccct
     3241 gcgccgcgcc cgtgagctgt tgctgccgct gcgcgaggaa gcgcgccggc ataacgccgt
     3301 gacccgtggc gcagccctgg ccctagcggc aatccgcaag aagggcctgg acgcagtacc
     3361 gcaggcgtca ctgccgctgt tcagtcgccc gcaaagcacc ttcctcggca ccgcttcgca
     3421 ggtcgaggcc tatgtatatg ccctggcccg tttcgagccc aagccggcgc agttccccaa
     3481 ggcgcatgcc agccgcaaag gcgaagcacc gcgcgcgcca cgcacagcgc gggagatgat
     3541 cgaacgctgc gagcaggccc tgccgctgcc ggacctgatg gtctggctgc tggaacagga
     3601 gccagaaggt gccaccgacg aattgctcta ctggttctcg cgcctgtccc gcgatggccg
     3661 cttccaacgt gagcgccttg agcgccgcga atacctcacc cgcgagcatc aggtcagcct
     3721 gagctccttt gccctgatca agccggccgg gaccaccgta tgaatatcga cctaaaagaa
     3781 ctgacccagc tcgccccgat cttccgcgag ctgttcaagg gctaccacat cagccgcagc
     3841 aacccggagc tgtatagcca gctggccagc caacaggacc agtaccgcgg cctgttcaag
     3901 gcgctgggct atgaactgac ctgcgacagc cgcggcttct actatttcgt ccccgagcag
     3961 atgggcaccc aggtcaacaa gactgcccag cgcctggcgc tgttcacctt tattctggtt
     4021 gaacatttag cggaccaggg ccgtgacccg ctggccgtac tcgacggcgg cagcctcggt
     4081 cgtgacgaac tgccagcgct gctggagaag taccgcgacc tattcctcca ggccgaagtc
     4141 accacccagg atgagctgga agagaaagtg atgcgccgcc tgacccagct gggttttgcc
     4201 agcgaagaca acggcattta ccgctttctc gcgccgatgc accgtttcct cgatgtgtgt
     4261 ctctccgtgc agcaagaccg cgacctggcc gccaccctgc acagcagcct gccgctgcca
     4321 acgccggtgc tgctggatga ggagaccgaa gacgaaatca tcagcctcgc agaaggcgat
     4381 gccgaagaat ccgaagaaga cgccctggcc cgcgccatgg ccgaagaacg tgacgccaag
     4441 gagaccctcg catgagccag gaacgctacg gcatccgccg cttcgcccta ttgaataccg
     4501 ccggctacag cctcggcctg ttccccctgg aaaacccgct gtcggtgtac ggggccaaca
     4561 acctgggtaa atccgcctcg atcaacgccc tacagttccc gattctcgcg cgcatgtcgg
     4621 acatgagctt cggcaagtac agcctggagc agtcgcgcaa gttctacttc gccagcgaca
     4681 ccagctatat cctcgtcgaa gtcgtcctac cccacggccc ccatgtgatc ggcgtggccg
     4741 ggcgcggccc aggtggcggc ttcggccacc agttcttcgc ctacaggggc accctggacc
     4801 tggagcacta tcagaagaac ggcacctgcc tgcgtcaacg cgagctgttc aacgcgctgg
     4861 aacgcgaagg catcaaggcc tatgaactga aaccggaaga gctgcgccgc ctgctggtcg
     4921 gcggccacac ttcgattccg ctagacctga ccctgatccc gctacgctcc accagcgagc
     4981 agagcctgaa gaccttccgc gcgctgttta tcaacctgct gcatatgcgc gaaatcaccg
     5041 cagccaagct taagcagctg ttcctcgacg ccttcgaaca cagcctgcgt tccggcagcg
     5101 tcgactatat cgccgccacc gaagaagcct tccgcgatgt acgccggatg gaaagcgact
     5161 atcaggccct ggttaccgcc ggccccttga tcgaagccct ggccgctggc gtggcccagc
     5221 gcgagctgct gcgcggcaag ctgcatcgcc tctcgccgct gctcgactca ctgctcggca
     5281 cctggcagga ctacgccggc gcgcgccgcg aagagctggt gatccaggcc gaacactacc
     5341 gcggcgagca ggacggcctg cagcacgagc aacgcggcgg cacccaggaa ctgatgcgca
     5401 tggagcgcga aatcagcgag atccagcgct ggctcggcga gctgtcggtg ctgaagaatc
     5461 gctttgccct ggtcaacgat gccaaggtgc tggaagaaca actgctggcc gccaaagacg
     5521 cccacgacga gctggcggga gccctggcgc aatcccggca gttctccgca gaagatctgg
     5581 atgagcgtct gcgtgatctg gataaacgcc tgaaatcggt aaaacagcaa ctcgaacacg
     5641 ccgacaacaa cagctactca cgcctgcgcg aagaattcag ccagcaggat gttgaccgcc
     5701 tgatgcgcct gttcaatggc cagctgttca gcctgccgct gggtgataaa ggcattcagg
     5761 tcgcagaggg tgacgcctgg gtcaaatccc tggaagccat tctcgatggc tttaagggcg
     5821 agcacttcga agtgccgggc ctgaccatcg acctctcgca tatcgagccc ccagctttgc
     5881 aagccctggc cgaccgcgcc gccctgcgtg accagaagga ccgtctggag cgcgaataca
     5941 aacaactgaa aacccagcag gcggtcgccg ccgaccgcgc cgcgagcaag gcccaggccg
     6001 agcagttcta ccaagccgta ctggatgcac agaaagccct ggaagacttc cgccgcagcc
     6061 aaaccctggc ggttgaagag ccgcagaaac tggagcagct gacgcagtta gaagccgccc
     6121 aggacgaact caagcgcgcc tccgatgcct ttaccgagcg cgtgcagcag ctctccgcca
     6181 agctgcaact ggtcggccgt cagctcgccg atctggaagc caaggaacgc accctggaag
     6241 acgccctgcg ccgtcgccag ctgctgccgg ccaacctgcc attcggcacg ccgtttaccg
     6301 acccggtgga cgacagcctg gacaacctgc tgccgctgct taacgactac caggacgcct
     6361 ggcagggcct gcagcgtatc gacgggcaga tcgaggcgtt gtatgcctag gtgcgcctga
     6421 aaggcgtggc caagttcgac agcgaagacg acgtcgagcg ccgcctgcac ctgctggtca
     6481 acgcttacgc ccaccgtcag gatgaagccc tcaccctggc caaggcgcgc cgcgctgcgg
     6541 ttaccgatat cgcccgtacc ctacgcaata tccgcagcga ctacgacaat ctggaacacc
     6601 agctggcgct gttcaaccgc gagatcaaca agcgtcaggt gtccaacctg gaaagcttcc
     6661 gcatcgtgct cgcaccgaac aaggatgcgc ttaagcatat cgaccagatc atccacagcg
     6721 ccggccagta cgaggaaggc gaaactcttt cggtgttcga cctgcagcag agcagcgaac
     6781 aggacagcaa gaacgaggaa gccaaggact acctgtcgcg cctggttgcc gccaaccata
     6841 accagctggg cttgaaggat ctgtttgaac tggccttcga gatcaccaag gtgcacggcc
     6901 aaccggtgat tcacaccgat atcgatggcg ctgcgtccaa cggcaccaca atgaccatca
     6961 aggcgctgac caacatgtac ctgttgctgc acctgatgga tcgggaccag gccggcaagg
     7021 tgcggctgcc ttactacttg gatgaggcgg cggatatcga tgagaagaac cagcaagcgc
     7081 tgatcgaaac cagcctgcag ttgggctttg tgccgattct cgcctcggtg aagcctcagg
     7141 tctcggccca tgtggccatc gacttggaag gcggcagcgg gccgaatggc atctatatcg
     7201 acgaagcgga ttggaaatat atccagcggc gcgacagtgc caagagcaag gcagaaacca
     7261 caaccgcagc aaccgagcca gcctaacgct ggctcaccag ccacaaacca caagggccgc
     7321 aattgcggcc cttgtggttt atttctccag cgcgatcttc ggcgcccagc gtaaccactc
     7381 ttcactcacc tcatcatgca gggcaaaagt ctgaccagcc tgcgccggcc cgcccatctg
     7441 cggattgtcc ggtgaggcga aggcgatgcc gccctccagc agagcttcca gtgactcggt
     7501 gcgcacctta accccgctga acagcccggc atccacaccg atgccgctgg cattccagaa
     7561 acggctgccg ctgcgcacca acggcgcata gcgcggctcg atcagcacat ggatcagcac
     7621 ccgatccgag gtcgggccaa gttcgaaatc agtcacctta cccactggca cttcacggta
     7681 gctgaccacc acacccggtt tgatcgaacc gcgtcgcgca gcactcaaca ccagacgcag
     7741 cccctgttca cgagcgttct gcgtcggggc cgattcggcc gccgtgaaac ttacttgcgc
     7801 cgcgccaggc ttgctcgctg gccgaacctc caggtactgc ccactgacca aggtttccag
     7861 attggccgct cgggtcaggc tgacctccgg tttgaccacc caaaactgcg tgcctgcccg
     7921 ggcaatctgc tgcgtcgcct gggtaatccg cgcatacaaa atcaccgcct gcagatcatc
     7981 cgtcagctca atgcgctcta ccttgcccac ctccaggccc ttgtatcgaa tcgccgtacc
     8041 agggccgaga ccatcaccac tgagcacccg aatagtcagc tctacgcctt tctgcaacgc
     8101 cagttcctga ctgctgtgca ggttaaagcg ctcgacccgc ctactgcctt tgggcgcatt
     8161 aggatttggc gtttcaaagg cgatgccgcc cgccagcaag gtttgcaatg actcgctctt
     8221 gacctcaaca ccggacagcc cgcccttcaa tgtaatgccg ctggcattcc agaagcgcgt
     8281 gctgctgttc accagatgcg catgctcagg ctcgatatgc acgccaaacg ccacctgctt
     8341 gtcatcacgc gacaactgaa aactctgcac cgtgccgacc ttaacctgct tgaacaggat
     8401 cggactgccc acttccagag agccgcgcgt gtcgctgaac aacaccaaat gcagccccgg
     8461 tgcagacagg tcgagcggtg gagctttcgc tctagcgaca aaatcacgca gcggtggatt
     8521 gccggcatca cctggccgga tggcgatgta gttgccctta accagcgcct ccaggccggt
     8581 tatgccggct agggaaatcg acggcttgac cacccagaag tcggtgcctt caaccaggta
     8641 gtcttcggta cgtggatcca gcatcaactc cacccgtgca ccactgagat cctgatcaac
     8701 cttgaaggtc ttcatatgac cgacctgaat acccttgtac atcaccggcg tacgaccagg
     8761 ctccaggccc tcaaattcat gcagtttgag cgatacgcga atgccggcct gggcgctgtc
     8821 gaagtcttcg tacaggcgaa atggcagagt cggatccgtc ggcgggctgt ccttgcgatg
     8881 ctccggcgtg gcgaaggcaa ttccgcccgc gacaatactc gccagagact cagtgcgcac
     8941 tttcactccc gataaaccgg catcaatgga gacgccgctg gcattccaga aacgcgtgtg
     9001 cttacgcacc agggaggcaa actctggctc aataaatacc ttcacctcga tcagattctg
     9061 ctcctcaacc agggcataac ttttcacccg gcccacttgg atctgcttgt agaacaccgg
     9121 gctgtcacgg ttgagcgagc caagccgctc ggccttgagg gtcaggtgca aaccaggcgc
     9181 tgtatcggcc aagggcggcg gctcgctcag cgcggtgaat ttacgcgtag gctcaccatc
     9241 acctgggctg gcggtgatgt agttacccga cactagggtt tccaatccag tgatacccgc
     9301 cagggaaacg ctcggcttga ccagccagaa ccgcgtattg ctgcgcaggt gctgctcaac
     9361 gcgcttgtcc atctccaggg tggcgcgcac accgcgccgc tcgccgctgt catccagctc
     9421 cagtgcaatt accttaccga tgggcatgcc cttgaagatc acctcagtct tgcccggctg
     9481 aatgccatca ccgctggcga acatcacctg aatctcgata cccgcttcgt tataggcacg
     9541 ccagcccagc cagccaccaa tcaacagcgc aatcagcggc aatacccaga tagccgacca
     9601 gttcgaagca gggcgcgctt tagccaacgg caaatcattc atggtggtca tccgagtccg
     9661 tgttatccca aatcaagcgg gggtcaaaag ttaaagccgc cagcatggtc aacaccacca
     9721 cgctggcaaa cgcggtagct ccataaccgg gaacaatgct cgccagactg ccgaaattaa
     9781 ccaacgccac cagtatcgcg atgacgaaga tgtccaacat cgaccagcgt ccgatccatt
     9841 caataaagcg gtacatcaac acgcgctggc gtgccgacat tggttgatgg cgctggactg
     9901 agtacagcaa caaggcaatt ccaaccaact tgaaggtcgg caccagaatg ctggcaataa
     9961 aaaccaccgc agcaatcggt aacatgccgt agttcaccaa ctcgataacg cccgacatga
    10021 tggtgtcgga ctggcctttg cccaacgagt tgaccgtcat gatcggcagc agattggccg
    10081 ggatatacag cacagcagcc gtcagcaaca acgcccaggt acgcaccagg ctgttgggac
    10141 gacgcgcatg cacatgccca ccgcagcgtg tgcagaattg cgcgccttgc ccacgctcta
    10201 tggggttgag ttggtgacac tcatggcaaa tcagcagccc tgcatcaatc gcccgcatgt
    10261 gtatcctctc ccgacaaggc ttcccagatc tgatgaggcg acatcgtcac ctccaaccag
    10321 acctggacca gcaacagcgc gataaaacac aacagcccca aaccaatgct caaatcggcc
    10381 agatcaatca gcttcacgat agccaccaag atgcccatta agtagacctc gagcatgccc
    10441 cactcgcgca tatggtgata aatgcgatag agcaacaggc cataactgcg accaatatcc
    10501 aggcggatac tcagcagcac agcgaactgg cacaacaact tgagcagcgg caccgccatg
    10561 ctgcagagaa acaccactac ggccacgctc tgcatgccgc tgttatagag cccgagtacg
    10621 cccgtccaga cggtgtcctc agtcgcctgc ccgagcagat ttaactgcat gatcggcaag
    10681 aaattagctg gcacatacaa caacaaagcc gcgattacca acgccatact ccggcgcacg
    10741 acctgagcac gatgggtgta cagctcatag ccgcagcgcg ggcattcaac tcgctcgcca
    10801 aaagcgacct ggggtttgcg cagcaataga tcgcactcat gacaggcaat cagttgctta
    10861 agctcaagct cggaaagtga tgcagagagt gccggatcgg ccatggaaaa agctctgata
    10921 aaagtacgcc taggcatcat agccatgcgc tgtattagtt gcctatcaaa ggctgcgctg
    10981 cgctcagttt gtttttcacc gcgcaaagta aaacccccga ccgttttcac gatcgggggt
    11041 tttggtatag gtgcttgacg atgacctact ctcacatggg gaaaccccac actac
//
LOCUS       DASRUM010000185        11879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_756492, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000185 DASRUM010000000
VERSION     DASRUM010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 11879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11879
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756492"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..113)
                     /locus_tag="VFF22_11845"
     tRNA            complement(38..113)
                     /locus_tag="VFF22_11845"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(78..80),aa:Ala,seq:ggc)
     gene            complement(403..1884)
                     /gene="gltX"
                     /locus_tag="VFF22_11850"
     CDS             complement(403..1884)
                     /gene="gltX"
                     /locus_tag="VFF22_11850"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005738107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZX17538.1"
                     /translation="MTTVRTRIAPSPTGDPHVGTAYIALFNLCFARQHGGEFILRIED
                     TDQVRSTRESEQQIYDALRWLGIEWSEGPDVGGPHGPYRQSERGHIYQKYSAELVEKG
                     HAFHCFCSAERLDKLRAEQMEAKQTPRYDGHCMHLPAAEVQQRLAAGEPNVVRMKVPS
                     EGVCVVPDMLRGEVEIPWDRMDMQVLMKTDGLPTYFLANVVDDHLMGITHVLRGEEWL
                     PSAPKLILLYEYFGWDKPQLCYMPLLRNPDKSKLSKRKNPTSITFYERMGFLPQAMLN
                     YLGRMGWSMPDEREKFSLDEMVEHFDINRVSLGGPIFDLEKLSWLNGQWMRELSVEEF
                     AAGAQKWAFNSDYLMQIAPHVQGRVETFSQIAPLASFFFSGGVAPDAALFEHKKLSAE
                     QVRQLMQLILWKLEALRQWEKDKITGCIQAVVEHLELKLRDAMPLMFAAITGHASSVS
                     VLDAMEIIGPDLTRFRLRQAIELLGGVSKKENKEWEKLLAAIA"
     gene            2130..3026
                     /locus_tag="VFF22_11855"
     CDS             2130..3026
                     /locus_tag="VFF22_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZX17539.1"
                     /translation="MSTPSRTRSLLLIVAFATIYIGWGTTYLANHFLLLEVPPFIIGC
                     LRFSIAGLLALVWVLLSGQLRMQRSDWGGVLIGSLALIAVGQGALIYANQYLPTGMLA
                     MLYTTLPLWSVALEWLLGERPPLWVMCGLALACGGIVLLMNNALDHSASAEQWFAGLL
                     VVFATLLWAGGAWLMRQRPPFRSSWLGLSLQMLIGALLLALFGLWHGDWQALDLRAIS
                     SNAWLWLAYLVLPVSLGVYPAYFWLLREVRPSLVSTFAFVNPVVALLLGYLLLSERLS
                     VMAMLACGITLGGVVLIIFGRR"
     gene            complement(3121..5136)
                     /gene="uvrB"
                     /locus_tag="VFF22_11860"
     CDS             complement(3121..5136)
                     /gene="uvrB"
                     /locus_tag="VFF22_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HZX17540.1"
                     /translation="MSEFQLVTRFQPAGDQPEAIRQMVEGLEAGLSHQTLLGVTGSGK
                     TFSIANVIAQIQRPTLVLAPNKTLAAQLYGEFKSFFPNNAVEYFVSYYDYYQPEAYVP
                     SSDTFIEKDASINDHIEQMRLSATKALLERPDAIIVTTVSCIYGLGSPESYLKMVLHV
                     DRGDKMDQRALLRRLADLQYTRNDMDFARATFRVRGDVIDIFPAESDLEAIRIELFDD
                     EVESISAFDPLTGEVIRKLPRFTFYPKSHYVTPREVLLEAVEKIKVELVERLEYLRST
                     NKLVEAQRLEQRTRFDLEMIIELGYCNGIENYSRYLSGRGPGEPPPTLYDYLPDEALL
                     VIDESHVSVPQVGAMYKGDRSRKETLVEYGFRLPSALDNRPMRFEEWEAASPQTIFVS
                     ATPGNYEAEHAGRVIEQLVRPTGLVDPQIEVRPALTQVDDLLSEIHKRVAIEERVLVT
                     TLTKRMAEDLTDYLADHGVKVRYLHSDIDTVERVEIIRDLRIGAFDVLVGINLLREGL
                     DMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNLNGRAILYADRMTGSMERAIGET
                     ERRRNKQIAFNEANGIIPKGVFKDVQDILEGATVPGSRSKKRKGMAQAAEESARYENE
                     LRSPSEITKRIRQLEEKMYALARDLEFEAAAQLRDEIQKLRDRLLQV"
     gene            5313..6509
                     /locus_tag="VFF22_11865"
     CDS             5313..6509
                     /locus_tag="VFF22_11865"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HZX17541.1"
                     /translation="MSLFSAVEMAPRDPILGLNEAFNADTRATKVNLGVGVYYNEEGR
                     IPLLRAVAEAEKARIEAHAPRGYLPIEGIAAYDSAVQKLLLGADSELIAAGRVITTQA
                     VGGTGALKTGADFLKRLLPNTVVAISDPSWENHRALFESAGFAVHNYRYYDAASHGIN
                     RAGMLEDLKNLPARSVVVLHACCHNPTGVDLSLDDWKAVLDVLREREHVPFLDIAYQG
                     FGDGIEQDAFAVRLFAASGIPFFVSSSFSKSFSLYGERVGALSIVTGSQEEASRVLSQ
                     AKRVIRTNYSNPPTHGATVVASVLNSPELRTLWEEELAGMRERIRGMRMAMVEQLAAL
                     NCKRDFSFVAQQRGMFSYSGLTAAQVERLKTEFGIYAVSTGRICVAALNQRNLPAVTQ
                     AIAAVL"
     gene            6573..6648
                     /locus_tag="VFF22_11870"
     tRNA            6573..6648
                     /locus_tag="VFF22_11870"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6606..6608,aa:Asn,seq:gtt)
     gene            6658..6734
                     /locus_tag="VFF22_11875"
     tRNA            6658..6734
                     /locus_tag="VFF22_11875"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6692..6694,aa:Pro,seq:tgg)
     gene            7011..7235
                     /locus_tag="VFF22_11880"
     CDS             7011..7235
                     /locus_tag="VFF22_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HZX17542.1"
                     /translation="MLKTVTFTLMHFCIAFGVTYALTGSIAASGLVAAIEPLCNSVGF
                     YFHEKVWQRIEGRSTTAAARPKHAWLHHQT"
     gene            7341..8900
                     /locus_tag="VFF22_11885"
     CDS             7341..8900
                     /locus_tag="VFF22_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019408944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta (1-6) glucans synthase"
                     /protein_id="HZX17543.1"
                     /translation="MPATSRFPAAPYVFACLLGLLALFGLWFGIGQPVELADAATPTH
                     KLQCASYTPFDKDQSPFDQPMQLRPERMEADLALLAERFECLRTYSVTGLEELPNMAR
                     KHGLKLIVGAWVSRNPADTAVEIAGLVKIANAHPDVVQAVIVGNEALLRKEVTPQQLV
                     ALIEQVKAQIEQPVTYADVWEFWLKHPEVAPAVDFITIHLLPYWEDNPAGIESALHEV
                     AEVRQTFGNAYAPKDILIGETGWPSEGRQRETALPSLVNQAKFIRGFVAMAEQNGWRY
                     NLIEAFDQPWKRVSEGAVGGYWGLYDADRQDKSILAGSVSNLPHWPAWLALSLGLFAA
                     TLLLAGRPHSPRAALLLPLLAALGAGCIGLFAELTLITSRYIGEWLWAAALILLNLLV
                     LAHAALLLSQQAGRRARLFNWLHSKGALWLALSGFAGAVLMLALVMDARYRSFPSAAL
                     LLPALVYLCRPVGGYRREVALLALLIGACIAPQLYQETLSNWQALGWALTCVLLVAAL
                     WRSLRVNAQTA"
     gene            complement(8901..9269)
                     /locus_tag="VFF22_11890"
     CDS             complement(8901..9269)
                     /locus_tag="VFF22_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014717724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17544.1"
                     /translation="MSRYLPLTALLIAAALCLALSFALRYQLMEGAQWVGLCADAPQQ
                     LACQVRASLGWLIHFRVLAWAALIIAVLAFVLPGAWGKRLAVPALLLALPALVLYSAS
                     LAVFAVVLALLRWVRAPQTA"
     gene            9374..9946
                     /locus_tag="VFF22_11895"
     CDS             9374..9946
                     /locus_tag="VFF22_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17545.1"
                     /translation="MLIRRLILITASLLGLWMLWVMLLPTPSSLPPSTPLSSGSWRVA
                     EHKVSRLESFQLEARILGREDYYFDRGAKLSPTDLALGWGPMADPEVLRHINISQRNR
                     WYFWRAEQLPIERRQIEIHSANMHLIPANPAVASTLAGLKEGQQIRLSGQLVRIDGDD
                     GYRWVSSLSREDTGDGACELIWVEQLAVLP"
     gene            10085..11383
                     /locus_tag="VFF22_11900"
     CDS             10085..11383
                     /locus_tag="VFF22_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HZX17546.1"
                     /translation="MSHVPRLLALLSCLALIAPVSAADIDASSYGYPISNPFEATLAT
                     TPPELRPELPADADINQSDYQIKLRPEREFELPDNFWPVTKMRYRMAKQSGEAPLIFI
                     IAGTGAHYASSTPEYLKKLFYGAGYHVVQLSSPTSYDFMTSASRFATPGITREDADDL
                     YRTMQAVRAQHPRLKVSEYHLTGYSLGALQAAFVSELDETRRSFNFKRVLLLNPPVNL
                     YTSITNLDRMVQTKVEGIDNSTTFYELVLGKLTRYFQNKGYVDINDALLYDFQNSGEQ
                     LSDEQMAMLIGAAFRFSSADIAFTSDLINRRGLITPIDYPISEGTSLTPFFRRALQCD
                     FDCYLTEQLIPMWRARTDGGSVAQLIDQVSLYGVADYLKNSSKIAVMHNADDVILGPG
                     DLGFLRTTLGDRLTVYPLGGHCGNLNYRVNSDAMLEFFRG"
     gene            11400..>11879
                     /locus_tag="VFF22_11905"
     CDS             11400..>11879
                     /locus_tag="VFF22_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HZX17547.1"
                     /translation="MFFTLLLGSSLTHAEHAVDDDGFKQPLQHLHFNPGLDQREFERS
                     TLSALHVYDPLESWNRRVYHFNYRFDEWVFLPIVDGYRYITPNLVQTGVSNFFSNLGD
                     VPNLLNSALQLKGKRAMQTTGRLLLNTTLGVAGLWDPATRMGLPKQREDFGQTLGYYG
                     "
BASE COUNT         2258 a         3830 c         3446 g         2345 t
ORIGIN      
        1 caagagctga ttgtggcaag cctttcggct ttgaagatgg tggagctaaa cgggatcgaa
       61 ccgttgacct cttgcatgcc atgcaagcgc tctcccagct gagctatagc cccatctcaa
      121 ggacggggcg catgttaaga gcacgcccca cacctgtcaa caaaattttt accgtcatca
      181 gcattcttta tgctgacaga acaaccactt accccatccc ggcacgccaa aacgacggcc
      241 ggatggggca ttcagctgct tagaacctgc ttcggagctc gcgagctaga gcgaggcaag
      301 gcaaaaatgg ccgaggaagc ggagtttacg ggctgtaaat gagcattccg aggccatttt
      361 taacgcagta tcgccgacgc gcagcagatc cgaaggaggt tcttaggcga tggccgccaa
      421 cagcttctcc cactctttgt tttctttctt cgacacgcca ccgagaagct cgatggcctg
      481 acgcagacgg aagcgagtca ggtccgggcc gataatttcc atggcatcca gcaccgacac
      541 cgaactggca tgcccagtga tagcggcaaa catcagcggc atggcatcgc gcagcttcag
      601 ctccagatgc tcgaccaccg cctggatgca gccggtgatc ttgtcttttt cccactgacg
      661 cagcgcttcg agcttccaca agatcagctg catcagctga cgcacctgct cggccgagag
      721 cttcttgtgc tcgaacagcg cggcatccgg ggccacaccg ccggagaaga agaagctggc
      781 caatggcgcg atctggctga aggtctccac cctaccctgc acatgcggtg cgatctgcat
      841 caggtagtcg ctgttgaacg cccacttctg cgcaccagcg gcgaattctt cgacgctcag
      901 ctcacgcatc cactggccgt taagccagga cagcttctcc aggtcgaaga tcggcccgcc
      961 gagggaaacc cggttgatgt cgaaatgttc aaccatctcg tcgaggctga acttctcgcg
     1021 ctcgtccggc atcgaccaac ccatgcgacc gaggtagttg agcatcgcct gcggcaggaa
     1081 gcccatgcgt tcgtagaaag tgatcgaggt cgggttcttg cgcttggaca gcttgctctt
     1141 atccgggtta cgcagcagcg gcatgtagca cagctgcggt ttgtcccagc caaagtactc
     1201 gtacagcagg atcagtttag gcgccgacgg cagccattct tcgccgcgca gtacgtgggt
     1261 gatgcccatc aggtggtcat cgaccacgtt ggccaggaag taggtcggca ggccatcggt
     1321 cttcatcagc acctgcatgt ccatgcgatc ccaggggatc tctacttcgc cacgcaacat
     1381 atccggcacc acgcaaacgc cttcgctcgg caccttcatg cgcactacgt tcggctcgcc
     1441 agcggcaaga cgctgctgca cctcggcggc tggcagatgc atgcagtggc cgtcataacg
     1501 cggcgtctgc ttggcttcca tctgctcggc gcgcagtttg tccaggcgtt cggcgctgca
     1561 gaagcagtgg aaggcatggc ccttctccac cagctcggcg ctgtacttct ggtagatatg
     1621 cccgcgttca ctctgccgat atgggccgtg cgggccgccg acgtccggac cttcactcca
     1681 ctcgatgccc agccagcgca gggcgtcgta gatctgctgt tccgactcgc gggtcgagcg
     1741 cacctggtcg gtatcttcga tacgcaggat aaattcgcca ccgtgctgac gggcaaagca
     1801 caggttgaac agggcgatat aggcggtgcc tacgtgtgga tcaccagtgg gcgacggcgc
     1861 gatacgggta cgaacggtgg tcatggcggc tctcagagaa tgtgcaacgg gcaatctttc
     1921 aagcccgcga tattaacagg ctggccgccg ccggctccag ccagcgcggc tatttcagct
     1981 gcgtccgcac ccgcgtcggg ttaacgcttt atcttgtagt gtgcagcagg ctggacaaca
     2041 acgtcagcgc gcttagactg gcgctctcta ctgcgggccc tgcagctttt tccgccaccc
     2101 gccctgccga ctcgtcaaat cagatcgcca tgtccacccc atcacgcacc cgcagcctgc
     2161 tgctgatcgt cgcctttgcc actatctata tcggctgggg caccacctac ctggccaacc
     2221 actttctcct gctggaagta ccgcccttta tcatcggctg cctgcgcttc tcgattgccg
     2281 gcctactcgc cctggtctgg gtgctgctca gtggccaact gcgcatgcag cgcagtgact
     2341 ggggcggcgt gctgattggc agcctggcct tgattgcggt cggccagggc gcactgatct
     2401 atgccaacca gtatttgccg accggcatgc tggcaatgct ctacaccacc ctgccgctgt
     2461 ggagcgtggc gctggaatgg ctgctcggtg agcgcccgcc gctgtgggtg atgtgcggcc
     2521 tggcgctcgc ctgcggcgga attgtgctgc tgatgaataa cgctctggac cacagcgcct
     2581 ccgccgaaca gtggttcgcc ggactgctgg tggtatttgc cacgttgctc tgggcaggtg
     2641 gcgcctggtt gatgcgccag cgcccgccat ttcgctccag ctggctgggg ctgagcctgc
     2701 agatgctgat cggcgcacta ctactggcgc tgttcggcct gtggcacggc gattggcaag
     2761 cactcgacct gcgcgccata agcagcaatg cctggctgtg gctggcctac ctggtgctgc
     2821 cggtcagtct gggcgtgtat ccggcgtact tctggctgct gcgcgaagtg cggccgagcc
     2881 tggtatcgac cttcgctttc gttaacccgg tggtggcgct gctattgggc tacctgctgc
     2941 tgagtgaacg cctgagcgtg atggcgatgc tggcctgcgg tattaccctg ggcggcgtgg
     3001 tgctgattat ctttggccgg cgttaatcgc aaacctgtgg gaggggctgg gcggcactcc
     3061 gctttagccg cgacagagcg acactgtttt cgcggctaaa gcccctccca cacgccccaa
     3121 ttaaacctgc agcagtcgat cacgcagctt ctggatttca tcacgcagct gcgcggcagc
     3181 ctcgaattcc agatcacggg ccagggcgta catcttctct tccagttggc ggatgcgctt
     3241 ggtgatttcg ctcggtgagc gcagctcgtt ctcgtaacgc gcgctttctt cggcggcctg
     3301 ggccatgccc ttgcgcttct tgctgcgcga gcctggcacg gtggcgcctt cgagaatgtc
     3361 ctgaacgtcc ttgaacacgc ccttggggat aatgccattg gcttcgttga aggcgatctg
     3421 cttgttacgg cggcgctcgg tctcgccgat ggcgcgctcc atcgagccgg tcatgcggtc
     3481 ggcatacaga atcgcccggc cattgaggtt acgcgctgcg cgaccgatgg tctggatcag
     3541 cgagcgctcg ctgcgtagaa agccctcctt gtccgcatcg agaatcgcca ccagcgacac
     3601 ttccggcata tccaggcctt cgcgcagcag gttgatgccc accagcacat caaaggcgcc
     3661 gatacgcaga tcgcggatga tctcgacccg ctccacggta tcaatgtccg aatgcaggta
     3721 gcgcaccttg accccgtgat cagccaggta atcggtcaga tcctcggcca tacgcttggt
     3781 cagcgtggtg accagtacac gctcctcgat ggccacgcgc ttgtggattt ccgacagcag
     3841 gtcatcaacc tgggtcagcg ctgggcgcac ttcgatctgc gggtcgacca ggccagtcgg
     3901 tcgcaccagt tgctcgatca ctcgaccggc atgctcggct tcgtagttgc ccggcgtggc
     3961 ggagacgaag atggtctgtg ggctggccgc ctcccactct tcgaagcgca tcggccggtt
     4021 atccagcgcc gaaggcaggc ggaaaccgta ttcgaccagg gtttccttgc gcgagcggtc
     4081 ccccttgtac atcgcgccga cctgcggtac cgacacgtgg gactcgtcga tcaccagcaa
     4141 cgcttcgtcc ggcaggtagt cgtacagcgt cggtggcggc tcgcccgggc cgcgcccgga
     4201 caggtagcgc gagtagtttt cgatgccgtt gcagtagccc agctcgataa tcatctccag
     4261 atcgaaacgg gtgcgctgct ccaggcgctg ggcttccacc agtttattgg tgctgcgcag
     4321 gtactccagg cgctcgacca gctcgacttt gatcttctcc accgcctcca gcagtacctc
     4381 gcgcggggtg acgtagtggc tcttggggta gaaggtgaag cgcggcaatt tgcggatcac
     4441 ctcgccagtc agcggatcga aggccgagat actttccacc tcatcatcga acagctcgat
     4501 acgaatggct tccaggtccg attccgccgg gaagatatcg atcacatcgc cgcgcacgcg
     4561 gaaggtggcg cgggcgaaat ccatgtcgtt gcgggtgtat tgcagatcag ccaggcggcg
     4621 cagcaaggcg cgctggtcca ttttgtcgcc gcgatcgaca tgcagcacca tcttcaggta
     4681 cgactcgggg ctgcccagac cgtagatgca cgacacggta gtgacgatga tcgcgtccgg
     4741 ccgctccagc agcgccttgg tcgccgacag gcgcatctgc tcgatatggt cgttgatcga
     4801 ggcgtccttc tcgataaagg tatccgagga cggcacatag gcttccggct ggtagtagtc
     4861 gtagtaggaa acgaaatact ccaccgcatt gttcgggaag aagctcttga actcgccgta
     4921 cagctgcgcg gccagggtct tgttcggtgc cagcaccagg gtcggacgct ggatctgcgc
     4981 gataacattg gcgatggaaa aggtcttgcc cgagcccgtt acgccgagca atgtctggtg
     5041 cgaaaggccc gcttccaggc cttcaaccat ctgccgaatc gcttcgggct gatcgccggc
     5101 cggctggaaa cgggtgacca attgaaactc ggacatgacg gacctcgaca agcggctgtg
     5161 acgcggcttg aaagcctacg tcaaaaccgg tgaaaagcgt gtgcctgctg tcgcagcaca
     5221 ggttatgcgt cggtatacta ccggcccact tacgcaaccc ctagcagggt gttgcctgat
     5281 actcacttct cctcttcaga gctgccgcat ccatgagtct gttctctgcc gtcgaaatgg
     5341 ctccgcgcga tcccatcctg ggcctcaatg aagcattcaa cgccgatacc cgtgcgacca
     5401 aggtcaacct gggcgtaggc gtttactaca acgaggaagg ccgcatccca ttgctgcgcg
     5461 ccgtagccga agccgagaaa gcccgcattg aagcccacgc cccgcgtggt tacctgccga
     5521 ttgaaggcat tgcagcctat gacagcgccg tgcagaaact gttgctgggc gcagactccg
     5581 agctgatcgc cgctggtcgg gtgatcacca ctcaggccgt gggcggcact ggcgcgctga
     5641 agaccggcgc tgacttcctc aagcgcctgc tacccaatac cgtggtcgcc atcagcgacc
     5701 cgagctggga aaaccaccgc gcgctgttcg agtccgccgg cttcgcggtg cacaactacc
     5761 gctattacga cgcagccagc cacggcatca accgcgccgg catgctcgaa gacctgaaaa
     5821 atctgccagc ccgctcggtt gtcgtgctgc acgcctgctg ccacaacccg actggcgtcg
     5881 acctgtcgct ggatgactgg aaagccgtgc tggacgtgct gcgcgagcgc gagcacgttc
     5941 cgtttctcga catcgcctac cagggctttg gcgacggtat tgagcaggac gcctttgctg
     6001 tgcgcctgtt tgccgcctcc ggcatcccgt tcttcgtctc cagctcgttc tccaaatctt
     6061 tctcgttgta cggcgagcgt gttggcgcgc tgtcgatcgt caccggcagc caggaagaag
     6121 ccagccgcgt gctgtcgcaa gccaagcgcg taatccgcac caactactcc aacccgccaa
     6181 cccacggcgc caccgtggtg gccagcgtgc tgaacagccc tgagctgcgc accctgtggg
     6241 aagaagagct ggccggcatg cgcgagcgta ttcgcggcat gcgcatggcc atggtcgagc
     6301 agctggcggc gctcaactgc aaacgcgatt tcagcttcgt cgcccagcag cgtggcatgt
     6361 tctcctactc gggccttacc gccgcgcagg tcgagcgcct gaaaaccgag ttcggcatct
     6421 acgccgtcag caccgggcgc atctgcgtgg ccgcactgaa ccagcgcaac ctgcctgctg
     6481 ttactcaggc catcgctgcc gttctctgag tgattgggaa gtcgatcagg tattgacttc
     6541 ccctttctaa tcagtaggat acgcgccatt gttccgcgat agctcagtcg gtagagcaaa
     6601 tgactgttaa tcattgggtc ccaggttcga gtcctggtcg cggagccagt agtacatcgg
     6661 ggtatagcgc agtccggtag cgcgcctgct ttgggagcag gatgtcggga gttcgaatcc
     6721 ccctaccccg accatttttg gtcgttggct cagctggtag agcagttggt gtttaaccaa
     6781 ttactcatga gtcagaaagc cacatgcacc accctttcaa gcctgcttga aagtaaaaac
     6841 ccgcctagtg cggatttttt attggctatt tatccgtaac ggctactcgg gtctgtcagc
     6901 tcaccaatca tcaaaaacta ttaaagccat tcaagcacaa catttcccca atcaagcttg
     6961 agcaactaga ctgcacccca atagagaaca attctcactc gaggtgcgcc atgctcaaga
     7021 ctgtgacttt caccctgatg cacttctgca tcgccttcgg cgtcacttac gcgttgaccg
     7081 gcagtattgc ggccagcggc ctggtggcgg ccatcgaacc gctgtgcaac tcggtgggtt
     7141 tctacttcca cgagaaggtc tggcagcgca tcgaaggtcg ctcaacaact gcagctgcac
     7201 gccccaagca tgcctggctg catcaccaga cgtaaggctt gatgccacag taaaaccctt
     7261 gaactgggcg taaaaacagc cagcgaggtg ctgggccaat ccgctactat gcgcggctca
     7321 gccaccatac gcgcccgatc atgcctgcta cttctcgctt ccctgctgcc ccctacgtgt
     7381 ttgcctgcct gcttggcctg ctggcgctct tcggtctgtg gtttggcatt ggccaaccgg
     7441 tcgaactggc ggatgccgca acgcccacgc acaagctgca gtgcgcgtcc tacacgccgt
     7501 tcgacaaaga ccaatcgccc ttcgatcagc caatgcagct gcgccccgag cgtatggaag
     7561 cggatctggc cttgctggcc gagcgtttcg agtgcctgcg tacctactca gtgaccggcc
     7621 tggaagagct gccgaatatg gcgcgtaaac atggcctaaa actgatcgtc ggcgcctggg
     7681 ttagccgtaa cccggccgac actgcggttg agatcgccgg cctggtgaaa atcgccaatg
     7741 cccacccgga cgtggtccag gcggtgatcg tcggtaacga agcgctgctg cgcaaggaag
     7801 tcaccccgca gcaactggtg gcgttgatcg aacaggtcaa ggcgcagatc gagcagccgg
     7861 tgacctatgc cgatgtctgg gagttctggc tcaagcaccc ggaagtcgcc cccgcagtgg
     7921 acttcatcac catccacctg ctgccctact gggaagacaa ccccgccggc attgaaagcg
     7981 cgctgcatga ggtcgccgag gtgcgccaga cctttggcaa cgcttacgcg cccaaagaca
     8041 tcctgatcgg cgaaaccggc tggccaagcg aaggccgtca gcgtgaaacc gccttgccaa
     8101 gcctggtcaa ccaggccaaa tttatccgtg gttttgtcgc catggccgag cagaacggct
     8161 ggcgctacaa cctgatcgaa gccttcgacc aaccctggaa acgcgtcagc gaaggcgccg
     8221 tgggcggtta ctggggcctg tatgacgctg atcgccagga caaatcgatc ctcgccggtt
     8281 ccgtatccaa cctgccgcac tggccggcct ggctggcgtt gagcctgggc ctgtttgccg
     8341 ccacgctgct gcttgccggc cgaccgcact cgccacgcgc cgcgctgttg ttgccgttgc
     8401 tggcggcgct gggcgccggc tgtatcggtc tgtttgccga attgacgctg atcaccagcc
     8461 gctacatagg cgagtggctc tgggccgccg cgcttattct gctgaatctg ttggtactgg
     8521 cccacgccgc cctgctgctc agtcagcagg ctggcaggcg cgcgcggcta tttaactggc
     8581 tgcatagcaa gggcgcactg tggctggcgc tgagcggttt tgccggggcg gtactgatgc
     8641 tcgcgctggt gatggatgcg cgctaccgca gcttccccag cgccgcgctg ctgctgccgg
     8701 cactggtcta cctgtgtcgc ccggtcggtg gctaccgccg tgaagtcgcc ctgctcgctc
     8761 tgctgatcgg cgcatgcatt gcgccgcagc tgtaccagga aaccctgagc aactggcagg
     8821 ccctgggttg ggcgctgacc tgcgtgctac tggtggcagc gctgtggcgc agccttcggg
     8881 ttaacgcgca aaccgcctga tcaggcggtt tgcggcgcgc gcacccagcg cagcagcgcc
     8941 agcaccacgg caaataccgc gaggctggcg ctgtacaaca ccagcgccgg cagagcgagt
     9001 agcaatgccg gcaccgccaa acgcttgccc caggcgcccg gcagcacaaa cgccagcacc
     9061 gcaataatca gcgcagccca ggccaagacc cggaaatgga tcaaccagcc aaggctcgcc
     9121 cgcacctggc acgccaactg ctgcggggcg tcggcgcaca ggccgaccca ctgcgcgcct
     9181 tccatcagct ggtaacgcag cgcaaagctc agtgccagac acagggctgc agcgatcagc
     9241 agagcagtca ggggcagata acgggacata ggcaactcaa gcagataaaa aggctgcgta
     9301 ggataagccg ccacggcacg gataacatcc cccgagatcg cctactcaca gcctctgcgg
     9361 ctagaatcca gccatgctca tacgtcgcct gatactgatc accgcaagcc tgctcggcct
     9421 ctggatgctg tgggtcatgt tgttgcccac gcccagcagc ctgccgccca gtacgccgtt
     9481 gtccagcggc agctggcgcg tggcggaaca caaagtcagc aggctggaga gtttccagct
     9541 ggaagcgcgc attctgggcc gtgaggacta ctacttcgac cgcggcgcca agctctcgcc
     9601 gaccgacctg gcgcttggct ggggaccgat ggctgatccc gaggtgctgc gccatatcaa
     9661 catcagccag cgcaatcgct ggtacttctg gcgtgcagag caattgccga tcgaacgccg
     9721 gcagattgag atccatagcg ccaatatgca cctgatcccc gccaacccgg cggtagccag
     9781 cacgctggcc gggctcaaag aaggtcagca gattcgcctg agcgggcaac tggtgcgaat
     9841 cgacggtgac gatggctatc gctgggtcag ctcgcttagc cgcgaagaca ccggcgacgg
     9901 cgcctgtgag ttgatctggg tcgagcaact ggcggtactg ccatagcaaa ttttcaacat
     9961 ggcgcttacg cctgaaaacc tgcacatcta taactaaacg gcacttagcc tgacttatgt
    10021 ggtcaaatcg ttccgcatct gaccgttgca tcagcatcag ccctagctct ccagagattt
    10081 agccatgtcg cacgttcctc gtctgcttgc ccttctcagt tgcctggccc tgatcgcccc
    10141 cgtcagcgcg gccgatattg acgccagcag ctacggctac cccatcagca acccattcga
    10201 agccaccctg gcgaccaccc cgccagagct gcgccccgag cttcctgccg acgccgacat
    10261 caaccagagt gactaccaga tcaaactgcg cccggagcgc gagttcgagc tgccagacaa
    10321 cttctggcca gtcaccaaga tgcgttaccg catggccaag cagtcgggcg aagcgccgct
    10381 gatctttatc atcgccggca cgggcgcgca ttacgccagc agcaccccgg aatacctgaa
    10441 gaagctgttc tacggcgccg gttatcacgt ggtgcaactg tcgtcgccca ccagctatga
    10501 ctttatgacc tcggcctcgc gctttgccac acccggtatc acccgcgaag acgccgacga
    10561 tctataccgc accatgcagg ccgtacgtgc gcagcatcca cgcctgaaag tcagcgaata
    10621 ccacctcacc ggctacagcc tgggcgcgct gcaggcggcc tttgtcagcg agctggatga
    10681 gacccgccgc agcttcaact tcaagcgcgt gctgctgctc aatccgccgg tcaacctcta
    10741 cacctcgatc actaacctcg accgcatggt gcaaaccaag gtcgaaggca tcgacaacag
    10801 caccaccttc tatgagctgg tactgggcaa gctgactcgc tacttccaga acaagggcta
    10861 cgtcgatatc aacgacgccc tgctctatga cttccagaac tccggcgagc agctcagcga
    10921 cgagcagatg gccatgctaa tcggcgcggc cttccgcttc tcctccgccg atatcgcctt
    10981 cacctcggac ctgatcaacc gccgcggcct gatcaccccg atcgactacc cgatcagcga
    11041 aggcaccagc ctcacgccgt tctttcgtcg cgccctgcag tgcgacttcg actgctacct
    11101 gaccgagcaa ctgatcccca tgtggcgggc tcgtaccgac ggcggcagcg tggcgcagct
    11161 gatcgatcag gtcagcctgt atggcgtggc cgattacctg aaaaacagca gcaagatcgc
    11221 cgttatgcac aacgctgacg atgtgatcct cggccctggt gacctgggct ttttgcgcac
    11281 caccctgggc gatcgcctga ccgtataccc gctgggtggc cattgcggca acctcaacta
    11341 ccgcgtcaat agcgacgcca tgctggagtt cttccgtggc taagccaatt tcccttgtcg
    11401 tgtttttcac cctgttgctg ggcagcagcc tgacgcacgc cgagcacgcc gttgatgacg
    11461 acggtttcaa gcagccgttg cagcacctgc acttcaaccc ggggctggat cagcgcgagt
    11521 tcgagcgctc gaccctcagc gcgctgcatg tgtatgaccc gctggagtcg tggaaccgcc
    11581 gcgtctatca cttcaactac cgcttcgatg agtgggtgtt cctgccgatt gtcgacggct
    11641 accgctatat caccccgaac ctggtgcaga ccggcgtcag caacttcttc agcaacctgg
    11701 gtgatgtgcc caacctgctc aacagtgcgc tacagctcaa gggcaagcgc gccatgcaga
    11761 ccaccgggcg cctgctgctt aacaccaccc taggagtcgc cggcctgtgg gacccagcca
    11821 cgcgcatggg cctgcccaag cagcgcgaag acttcggcca gaccctcggc tattacggc
//
LOCUS       DASRUM010000186        12353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2113021, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000186 DASRUM010000000
VERSION     DASRUM010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 12353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12353
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2113021"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..103)
                     /locus_tag="VFF22_11910"
     CDS             complement(<1..103)
                     /locus_tag="VFF22_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019472117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZX17548.1"
                     /translation="MWKVLPVTQKPDQCLGEWIDREALAEAMIPMIGQ"
     gene            250..3687
                     /gene="mfd"
                     /locus_tag="VFF22_11915"
     CDS             250..3687
                     /gene="mfd"
                     /locus_tag="VFF22_11915"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HZX17549.1"
                     /translation="MSVLRLPSLPATAGKQHWGNLPGAALSLAIAEAASNAKRFTLLL
                     TADSQSAERLQEELSFFAPDLPVLHFPDWETLPYDLFSPHQDIISQRIAALYRLPELK
                     HGVLVVPITTALHRLAPKRFLLGSSLVMQVGQQLDVNQMRGNLEAAGYRCVDTVYEHG
                     EFAVRGALIDLFPMGSSQPYRIDLFDDEIETLRTFDPENQRSIDKVDSIKLLPAREFP
                     LEKKAVTDFRGRFRERFDVDFRRCPIYQDLSTGITPAGIEYYLPLFFEETATLFDYLP
                     TDTQVFSLPGIEKAAEQFWVDARSRYEDRRVDPERPLLPPADIFLPVEDCFARLKNWP
                     RVVVSQDDIEEGVGRLRFNAKPLPDLAIQAKAGEPLAALRRFIEEYPGRVLFCAESAG
                     RREVLLELLARLKLKPNEFESWPAFTASKQRLGICIAPLDDGLLLEDLALVAESPLFG
                     QRVMQRRRREKSRDGGDNVIKNLTELREGAPVVHIDHGVGRYLGLVTMEFDGQAAEFL
                     ALMYAEEAKLYVPVASLHLIARYTGSDDALAPLHRLGSEIWQKAKRKAAEQVRDVAAE
                     LLDIYARRAAREGYAFADPKADYATFSAGFPFEETPDQQSAIDAVHDDMLAPKPMDRL
                     VCGDVGFGKTEVAMRAAFIAVHGGKQVAVLVPTTLLAQQHYNSFRDRFADWPVKVEVM
                     SRFKSAKEVSEAVQQLAEGKVDIVIGTHKLLQEDVKFSNLGLVIIDEEHRFGVRQKEQ
                     LKALRSEVDILTLTATPIPRTLNMAVAGMRDLSIIATPPARRLSVRTFVMEQNNPTIK
                     EALLRELLRGGQVYYLHNDVKTIEKCAADLAELVPEARIGIGHGQMRERELEQVMGEF
                     YHKRFNVLIASTIIETGIDVPSANTIIIERADKFGLAQLHQLRGRVGRSHHQAYAYLM
                     TQPRKQMTDDAQKRLEAISGAQDLGAGFLLATHDLEIRGAGELLGDGQSGQIQAVGFT
                     LYMEMLERAVKSIRKGEQPNLDQPLGGGPEINLRVPALIPEDYLPDVHARLILYKRIA
                     NAADEDGLKELQVEMIDRFGLLPDPSKNLIRITLLKLQAEKLGIKKVDAGPQGGRIEF
                     AADTCVDPLTLIKLIQSAPNRYKFEGATMFKIQVPMERPEERFNTLEALFERLTPST"
     gene            3699..4238
                     /locus_tag="VFF22_11920"
     CDS             3699..4238
                     /locus_tag="VFF22_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsiV family protein"
                     /protein_id="HZX17550.1"
                     /translation="MRALRTLALLPLILLGLLSSTAALAESLYQVEVIVFRQAATPIS
                     AGQLPPDNWAQNALPVDGSNSRSTALNAEAGKLSPSSGYQVLLHKAWSQSLGESNSRV
                     AISTGEEQFGHYPVEGTISLKAGRVIELDSDIWVNQLDASGILNDSERIKQSSRLKSG
                     ELTFVDNGSLGLLIRVSPL"
     gene            complement(4300..5037)
                     /locus_tag="VFF22_11925"
     CDS             complement(4300..5037)
                     /locus_tag="VFF22_11925"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HZX17551.1"
                     /translation="MTVYAIIGGTGLTKLDGLTIKAALHIDTPYGAPSAAVLKGDYAG
                     REVLFLARHGHPHRIPPHQVNYRANLWALREAGAKAIIAVNAVGGIHSAMGTGHFCLP
                     HQIIDYTYGREHTYFEGELEHVTHVDFSHPYDAGLRTKLANALTAEHCAFSNHGLYGC
                     TQGPRLETAAEIARMERDGCDIVGMTGMPEAVLARELALPYACLALVVNPAAGKSRAL
                     ITMAEIERALEEGMGTVKAVLARVLAS"
     gene            complement(5047..6045)
                     /gene="nagZ"
                     /locus_tag="VFF22_11930"
     CDS             complement(5047..6045)
                     /gene="nagZ"
                     /locus_tag="VFF22_11930"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HZX17552.1"
                     /translation="MQGSLMLDIAGTWLTAEDRQILRQPQVAGLILFARNIEDPRQVR
                     ELSAAIRAVRPDLLLAVDQEGGRVQRLRQGFVRLPAMRALADNANAEHLAEQCGWVMA
                     TEVLAAGLDLSFAPVLDLDHQRSAVVGSRSFEGDAQRATLLAGAFIRGMNAAGMAATG
                     KHFPGHGWAEADSHVAIPVDERSLEQIRAVDLQPFAQLSRQLAAVMPAHVIYPQVDSQ
                     PAGFSRRWLQDILRGELAFDGVIFSDDLSMAGAHVVGDAACRIEAALAAGCDMGLVCN
                     DRAAAELALSALQRLKVEPPQGLARMRRQAFPGIEYRQNPRWLAALGSLRDAQLID"
     gene            complement(6067..6591)
                     /locus_tag="VFF22_11935"
     CDS             complement(6067..6591)
                     /locus_tag="VFF22_11935"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HZX17553.1"
                     /translation="MVALDFLHISLADQCLYGFANGQLQLRLSVSTALNGAGERNGSG
                     CTPRGLHKVRAKIGEGLPSAAVLRGRRWTGEVWSPELHAQFPGRDWILTRILWLSGCE
                     PGRNRLGTVDTFRRYIYIHGTPDSEPMGVALSHGCVRLRNSDLLELFARVPVQCAVQI
                     DEAACPEWATAPLI"
     gene            complement(6639..7346)
                     /locus_tag="VFF22_11940"
     CDS             complement(6639..7346)
                     /locus_tag="VFF22_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZX17554.1"
                     /translation="MAQSETVERILDAAEQLFAEKGFAETSLRLITSKAGVNLAAVNY
                     HFGSKKALIQAVFSRFLGPFCVSLERELDRRQAKPETKVSLEELLELLVEQALAVKPR
                     SGNDLSIFMRLLGLAFSQSQGHLRKYLEEVYGKVFRRYMQLVHEAAPRIPPLELFWRV
                     HFMLGAAAFSMSGIKALRAMSETDFGVNTSIEQVMRLMVPFLAAGMRSETGLTDEALA
                     SAQLKPRTKTPAIPSKV"
     gene            7619..8230
                     /gene="lexA"
                     /locus_tag="VFF22_11945"
     CDS             7619..8230
                     /gene="lexA"
                     /locus_tag="VFF22_11945"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZX17555.1"
                     /translation="MLKLTPRQAEILAFIKQCLEDNGYPPTRAEIALKLGFKSPNAAE
                     EHLKALARKGAIEMTPGASRGIRIPGFEPNAEEDEGLPVIGRVAAGAPILAQQHVEES
                     CKINPDFFHPKADYLLRVRGMSMKDIGIFDGDLLAVHTTREARNGQVVVARLDDEVTV
                     KRFKREGNKVWLIAENPEFAPIEVNLEEQDLIIEGLSVGVIRR"
     gene            8241..8717
                     /gene="sulA"
                     /locus_tag="VFF22_11950"
     CDS             8241..8717
                     /gene="sulA"
                     /locus_tag="VFF22_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005765051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HZX17556.1"
                     /translation="MQFPQSLNRTQLPLFDAFLASTVTPLLADSAQLPWQDDETEAFS
                     ELSLRGAAGHCLNLLAPILRELSENHDERWLTLIAPPPSLTQTWLREAGLNRERILLL
                     HPRGAQSALELAQQALTLGRSHTVVSWLHPLERQARQQLEQAARQGKAQCLNISLG"
     gene            complement(8829..9062)
                     /locus_tag="VFF22_11955"
     CDS             complement(8829..9062)
                     /locus_tag="VFF22_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17557.1"
                     /translation="MPTSFLEIVELPDGRIALRRAEDEESLVVLDFSADAKAFLQGQH
                     VEVAKAMLNVGVQMAGRLAENNFQQEEGPRVLH"
     gene            complement(9141..9659)
                     /locus_tag="VFF22_11960"
     CDS             complement(9141..9659)
                     /locus_tag="VFF22_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6586 family protein"
                     /protein_id="HZX17558.1"
                     /translation="MANELYTRTNQKIFYAGLALESWRKAEQGQSMNALALIQAEREA
                     ALFHLYGALLGLCHEIAGYYRLPDSAAPQAERFLTEEVLQAAPSQELGELVELAQSAD
                     SWVAQLLKAYAELFQPPQAGKKAKVDPATPLITAVSLDEEVAPLAQETLEAWRQQLKA
                     LAQRFRQTLSEW"
     gene            complement(9731..12340)
                     /gene="topA"
                     /locus_tag="VFF22_11965"
     CDS             complement(9731..12340)
                     /gene="topA"
                     /locus_tag="VFF22_11965"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014852237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HZX17559.1"
                     /translation="MGKSLVIVESPAKAKTINKYLGNEYVVKSSIGHIRDLPTSGSAS
                     TAKEPVKRGKAAAGEVPALSPKEKAKRQLFARMGVDPEHGWKAKYEILPGKEKVVEEL
                     RRLAKDADTIYLATDLDREGEAIAWHLRESIGGDDSRYKRVVFNEITKKAIQEAFSKP
                     GELDINRVNAQQARRFLDRVVGYMVSPLLWAKIARGLSAGRVQSVAVKLVVEREKEIR
                     AFVPEEYWEVHADLGTAKGANVRFEVARENGAAFKPLNEAQAMAALATLKASSYNISK
                     REDKPTSSKPSAPFITSTLQQAASNRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLS
                     ADAVSMVRDFIEGEFGKKYLPANPIAYSSKEGAQEAHEAIRPSDVNLKPTQLSGMERD
                     AERLYELIWRQFVACQMPPAEYLSTTVTVTAAQFELRAKGRILKFDGYTRVLPQLSKP
                     GDDDVLPEMNQGEVLKLVKLDPSQHFTKPPARYSEASLVKEMEKRGIGRPSTYAAIIG
                     TIQDRGYVALHNRRFYSEKMGDIVTERLAESFSNLMDYGFTAGMEEHLDDVAQGEREW
                     KHVLDEFYGDFKKKLEVAEAGEGGMRANQPTLTDIACRDCGRPMTIRTASTGVFLGCS
                     GYALPPKERCKATVNLVPGDEIAADDEGESESRVLLGKHRCPICSTAMDAYLLDETRK
                     LHICGNNPDCSGYEVEQGQYRIKGYEGPSLDCDKCGSQMQLKTGRFGKFFGCTNSECK
                     NTRKLLKSGEAAPPKMDPVKMPELKCEKVNDTYILRDGASGLFLAASQFPKNRETRAP
                     LVLELIPHRDEIDPKYHFLCDAPKKDPQGRAAVIRYSRKTKEQYVQTEVDGKPTGWRA
                     FFDGGKWKVEDKS"
BASE COUNT         2567 a         3961 c         3511 g         2314 t
ORIGIN      
        1 gttggccaat catcggaatc atcgcttccg caagggcttc tcgatcgatc cactcaccga
       61 gacactggtc gggcttctga gtcacgggca gtaccttcca catgtagggg ctgaaaaaag
      121 gggctacatt atgacggcgc aagcaagcat gagcaatgca cgactgtcgt aactgacacc
      181 gcatataaac gcccgctaca atcgccgacc ttgtgccatg accgtgagcc gcccgtgtct
      241 gaaccgaccg tgtccgtact gcgtctaccg tccctaccgg ccactgccgg caaacaacac
      301 tggggcaacc tgcccggtgc cgcgctgagc ctggcgattg ccgaagccgc cagcaatgcc
      361 aaacgcttca ccctgctgct gactgccgac agccagagcg ccgagcgcct gcaagaagag
      421 ctgagctttt tcgctccgga tttgccggtg ctgcattttc ccgactggga aaccctgccc
      481 tacgacctgt tttcgccgca ccaggacatt atttcccagc gcatcgccgc gctttatcgc
      541 ctgcccgagc tgaaacacgg tgtattagta gtcccgatca ccaccgccct acaccgcctg
      601 gcgcccaagc gctttctgct cggcagcagc ctggtcatgc aggtcggcca gcaactcgac
      661 gtcaaccaga tgcgcggcaa tctggaagcc gccggctatc gctgcgtcga tacggtgtac
      721 gaacatggcg aattcgcggt acgcggcgcg ctgatagacc tgttccccat gggcagcagc
      781 cagccatacc gcatcgacct gttcgatgac gaaatcgaaa ccctgcgtac ctttgatcca
      841 gaaaaccagc gttccatcga caaggtcgac tcgatcaagc tgctgccggc gcgcgagttt
      901 ccgctggaga agaaagccgt taccgacttc cgcgggcgct ttcgcgagcg cttcgacgtg
      961 gatttccgcc gctgcccgat ctatcaggac ctcagcaccg gcatcactcc ggccggcatc
     1021 gagtactacc tgccgctgtt cttcgaagaa accgccaccc tcttcgacta tttgcccacc
     1081 gacacccagg tgttttccct accgggcatc gaaaaagccg ccgagcagtt ctgggtggat
     1141 gcgcgcagcc gctatgaaga tcgccgcgtc gacccggaac gcccactgct gccgccggcc
     1201 gacattttcc tgccggtgga agactgcttc gcccgcctga aaaactggcc acgcgtggtg
     1261 gtcagccagg acgatatcga agaaggcgtc ggccgcctgc gtttcaacgc caaaccgctg
     1321 cccgacctgg cgatccaggc caaggccggc gaaccgctgg cggcacttcg acgctttatc
     1381 gaggaatacc ctggccgcgt gctgttctgc gccgagtccg ccgggcgccg tgaggtgctg
     1441 ctcgagttgc tcgcgcgcct caagcttaaa cccaacgaat ttgaaagctg gccggccttt
     1501 accgccagca aacaacgctt agggatatgc atcgccccac tggatgacgg cctgctgctc
     1561 gaagacctgg ccctggtggc ggaaagcccg ctgtttggcc agcgcgttat gcagcgccgg
     1621 cgccgcgaga aatcccgcga tggcggcgac aacgtcatca agaaccttac cgagctgcgc
     1681 gaaggcgctc cggtggtgca tatcgaccac ggcgtcggcc gctacctggg cctggtgacc
     1741 atggagttcg acggccaggc cgccgaattc ctcgccctga tgtacgccga ggaagccaag
     1801 ctctacgtgc cggtagccag cctgcacctg atcgcccgct acaccggcag cgacgacgca
     1861 ctcgccccgc tgcaccgttt aggctcggaa atctggcaaa aggccaagcg caaagctgcc
     1921 gaacaggtgc gcgatgtcgc cgccgaattg ctcgacatct acgcccgccg cgccgcccgt
     1981 gaaggctatg cgtttgccga cccgaaggcc gattacgcca ccttcagcgc cggctttccc
     2041 ttcgaggaaa ccccggacca gcaatccgcc atcgacgcgg tgcatgacga catgctcgca
     2101 cccaaaccga tggaccgcct ggtctgcggc gatgtcggtt tcggcaagac cgaagtggcg
     2161 atgcgcgcgg cctttatcgc cgtgcatggc ggcaagcaag tggcggtact ggtaccgacc
     2221 acattgctcg cccagcagca ctacaacagc ttccgcgacc gcttcgccga ctggccggta
     2281 aaagtcgagg tgatgagccg cttcaaaagc gccaaggaag tcagcgaggc cgtgcagcaa
     2341 ttggccgaag gcaaggtgga tatcgtgatc ggtacccaca agctgctgca ggaagacgtc
     2401 aaattcagca acctgggcct ggtgatcatc gacgaagagc accgtttcgg cgtgcgccag
     2461 aaggaacagc tcaaggccct gcgcagcgaa gtcgacatcc tcacgcttac cgccacacca
     2521 attccacgca ccctgaacat ggccgtggcc ggcatgcgtg acctgtcgat catcgccacg
     2581 ccgccggcgc gtcgtctgtc ggtgcgcacc ttcgtcatgg agcagaacaa cccgacgatc
     2641 aaggaagccc tgctgcgtga gctgctgcgc ggcggccagg tgtactacct acacaacgat
     2701 gtgaaaacca tcgagaaatg cgccgccgat ctggccgaac tggtgcccga agcacgcatc
     2761 ggcatcggcc acgggcagat gcgcgagcgc gagctggaac aggtgatggg cgagttctac
     2821 cacaagcgct tcaacgtgct gatcgcctcg accatcatcg aaaccggtat cgacgtgccg
     2881 agcgccaaca ccatcatcat cgaacgcgct gacaagttcg gcctggccca gctacaccag
     2941 ttgcgcggcc gcgtgggtcg tagtcaccac caggcctacg cctacctgat gacccagccg
     3001 cgcaagcaga tgactgacga cgcgcagaag cgcctggaag ccatctccgg cgcccaggac
     3061 ctcggcgccg gcttcctgct ggccacccat gacctggaaa tccgcggcgc cggcgagctg
     3121 ctcggcgatg gccagagcgg gcagatccag gcggtcggtt tcaccctcta tatggaaatg
     3181 cttgaacgtg cggtgaaatc catccgcaag ggcgagcagc ctaacctcga ccagccgctt
     3241 ggcggcggcc cggagatcaa cctgcgtgtg ccggcgttga tccccgagga ttacctgccc
     3301 gacgtacatg cgcggctgat tctctacaaa cgcatcgcca acgccgccga cgaggatggc
     3361 ctgaaagagc tgcaagtgga gatgatcgac cgcttcggcc tgctgccgga tccgagcaag
     3421 aacctgatac gcatcaccct gctgaaattg caggcggaaa agctcggaat caagaaggtc
     3481 gatgccggcc cgcaaggcgg acgtatcgaa ttcgccgcag atacctgcgt cgatccgctg
     3541 accctgatca agctgatcca gagcgcgccg aaccgttaca agttcgaagg cgcgacgatg
     3601 tttaaaatcc aggtgcccat ggagcgcccg gaagaacgat tcaatacctt agaggcgctg
     3661 ttcgagcgtc tgactccctc tacctaaagg actgacccat gcgcgcactc cgcaccctgg
     3721 ccctactgcc gcttatcctg ctgggcctgc tcagcagcac tgccgccctg gctgaaagcc
     3781 tgtatcaggt tgaagtcatc gtattccgcc aggccgccac gccgatttcc gccggccagc
     3841 tgccgccgga caactgggcg cagaatgcac tgccggtgga cggcagcaac agccgcagca
     3901 ccgcactgaa tgccgaagcc ggcaaactca gcccgtccag cggctatcag gttctattac
     3961 acaaagcctg gtcccagagc ctgggcgagt cgaacagccg cgtcgccatc agcaccggcg
     4021 aagagcagtt cggccactac cccgtcgaag gcaccatcag ccttaaagcc ggccgcgtga
     4081 tcgagctgga cagcgacatc tgggtcaatc agctcgacgc cagtggcatc ctcaatgaca
     4141 gcgagcgcat caagcagagc agccgcctga aaagcggcga gttgaccttt gtcgataatg
     4201 gcagcctggg tctgctgatc cgcgtcagcc cgctgtaaga acacagacac aaaaaagggc
     4261 cgcattgtgc ggcccttttt tattgcagca atggccagct cagctggcca acacccgcgc
     4321 caatacggcc ttgaccgtac ccatgccctc ttccagggcg cgttcgattt ccgccatggt
     4381 gatcagcgca cgggacttgc ccgcggccgg gttcaccact agcgccaggc aggcgtaggg
     4441 caaagccagt tcacgagcca gaacagcttc cggcatgccg gtcataccga cgatgtcgca
     4501 accatcgcgc tccattcggg cgatttccgc tgcggtttcc aggcgcgggc cctgggtgca
     4561 gccgtacagt ccatgattgc tgaacgcaca gtgttcggca gtcagtgcat tggccagttt
     4621 ggttcgcagc cctgcgtcat agggatggct aaaatcgaca tgggtgacat gctccagctc
     4681 accctcgaaa taggtgtgct cgcggccgta ggtgtaatcg atgatctggt gcggcaggca
     4741 gaagtgtccg gtgcccatgg ccgagtgaat accgcccacc gcgttcacgg cgatgatggc
     4801 cttggccccc gcctcacgca gcgcccagag gttggcgcgg tagttgacct gatgcggcgg
     4861 aatacggtgc ggatgcccgt ggcgcgcgag aaacagcacc tcgcggccgg catagtcgcc
     4921 cttgagcacc gccgctgagg gcgcgccata gggcgtatcg atatgcagcg cggccttgat
     4981 ggtcagacca tccagcttgg tcagccccgt accgccgata atcgcgtaaa ccgtcatccc
     5041 ttactcctag tcgattaatt gcgcatcgcg caggctgcca agtgccgcca gccagcgcgg
     5101 gttctgccga tactcaatac ccgggaaagc ctgccggcgc atccgcgcca gaccttgcgg
     5161 cggctccacc ttgagtcgct gcaaggcgct cagggccagc tctgccgctg cccggtcatt
     5221 acacaccagg cccatatcac aacccgctgc cagcgccgcc tcgatacggc aagccgcatc
     5281 accaaccaca tgggcgccgg ccatggacaa atcgtcgctg aaaatcaccc catcaaaggc
     5341 cagctcgcca cgcagaatgt cctgcaacca gcgccgggaa aatcccgctg gctggctgtc
     5401 cacttgaggg tagatcacat gggcgggcat caccgcagcc aactgccggc tcaactgggc
     5461 aaagggctgc aagtcgactg cacgaatctg ttccaggctg cgctcatcga cagggatggc
     5521 cacatgggaa tccgcctcgg cccagccgtg cccggggaaa tgcttgccgg tagcggccat
     5581 gcccgcggcg ttcatcccac ggataaaagc tcctgcgagc aaggtcgcac gctgcgcatc
     5641 gccctcaaag gagcggctgc cgaccaccgc actgcgctga tgatcaagat ccagcaccgg
     5701 ggcgaaactc aggtccaagc ctgcagccag cacttcggtc gccatgaccc aaccgcattg
     5761 ctcggccagg tgctcggcat tggcgttatc ggccaacgca cgcatcgccg gcaagcgcac
     5821 aaagccctga cgcagacgct gcacgcgccc tccctcctga tccaccgcca gcaacaggtc
     5881 cggccgcacc gcgcgaatcg cagcggacag ctcgcgcacc tggcgcgggt cttcaatatt
     5941 gcgggcaaac agaatcaacc cggctacctg cggctgacgc aggatctggc gatcttcagc
     6001 ggtcagccaa gtgccggcga tatccagcat caatgaacct tgcatgcgtg tactcacaac
     6061 ccatccttaa ataagaggcg ccgtagccca ctcggggcag gcggcctcgt caatctgtac
     6121 cgcacattga accgggacac gcgcaaagag ttccagcagg tcgctgttgc gcaggcgcac
     6181 gcagccatgc gacagcgcca cgcccatagg ctcgctgtcc ggcgtaccgt gtatgtagat
     6241 atagcggcga aaggtgtcga cagtgcccag acgattgcgt ccaggctcac aaccactcag
     6301 ccagagaatg cgcgtgagaa tccagtcacg cccagggaat tgcgcatgca actcgggcga
     6361 ccaaacttcg ccggtccagc gcctgccgcg cagtacggcg gcgctcggca agccctcacc
     6421 aatcttcgcc cgcaccttgt gcaggccacg cggcgtacag ccagaaccat tgcgctcacc
     6481 ggcgccattc aacgcagtgg aaacactcag gcgcagctgc agctgcccat tggcaaagcc
     6541 atacaggcac tgatcggcga gggaaatatg cagaaaatcg agagcaacca tgggcggcta
     6601 gcttagcgga tcaaatccac caagcccacc cgccgaggtc aaactttgct tgggatggct
     6661 ggcgtcttgg tgcgcggctt gagctgtgcg ctggccaggg cttcatcggt caggccagtt
     6721 tccgaacgca tgccggcagc cagaaatggc accatcaggc gcatcacctg ctcaattgag
     6781 gtattaactc cgaagtcggt ttcagacatg gcccgcaagg ccttgatccc ggacatgctg
     6841 aacgctgcgg cgcctagcat aaagtgcacg cgccagaaca gttccagcgg cgggatacgc
     6901 ggagcagctt catgcaccag ctgcatgtag cggcggaaca ccttgccgta cacctcttcg
     6961 aggtacttac gaaggtgacc ctggctctgg ctaaaagcca gacccagcaa acgcataaag
     7021 attgagaggt cgttaccact gcgcggtttt actgccagcg cctgctccac caataactca
     7081 agcagctctt caaggctgac cttggtttcc ggcttggcct gacggcgatc cagctcacgc
     7141 tccaggctta cgcagaaagg cccgagaaag cgcgaaaaca ccgcctgaat cagggccttt
     7201 ttcgagccga agtggtaatt caccgcagcc agatttaccc cggccttgct ggtgatcaaa
     7261 cgcagagacg tttctgcgaa acctttctcc gcaaaaagct gctctgccgc atcgagaatg
     7321 cgttcaacgg tttccgactg ggccatgact cttttcatct gaacaaacac ttgtttgaaa
     7381 catacgtttc agctgcccac cttgtcaagc cgggcaaacc atcttgtggc cgggtggtca
     7441 cagacatccg gcaaagtcac aggctaaccc tagctagacg ccccatagac ggcccgagta
     7501 acagtgcact gcaacagacg atatgaaggt caattgagta gagatcagaa cacaaggatt
     7561 gccaatcgcc gatcactgta tataatccca gtcactgtac aaaaaaacag agccgaccat
     7621 gctaaaactg acgccgcgcc aagctgaaat tctcgctttc atcaagcagt gccttgaaga
     7681 caacggctac ccgcctaccc gtgcggaaat cgccttgaag ttggggttca aatcacccaa
     7741 cgcagcggaa gagcacctca aggccctggc ccgtaagggt gccatcgaaa tgacccctgg
     7801 cgcttcacgc ggcatccgca ttcctggctt cgagcccaat gccgaggaag atgaaggcct
     7861 accggtgatt ggccgcgtcg ctgcaggcgc accgattctg gcgcagcaac atgtcgaaga
     7921 gtcctgcaag atcaatccag acttcttcca ccccaaggct gattacctgc tgcgtgtacg
     7981 cggcatgagc atgaaggaca tcggcatctt cgatggcgac ctgctggccg tacacaccac
     8041 ccgtgaagcc cgtaatgggc aagtggtggt ggcgcgtctc gacgatgaag tgacggtcaa
     8101 acgtttcaag cgtgaaggca acaaggtctg gctgattgcg gaaaacccgg agttcgcgcc
     8161 gatcgaagta aacctggaag aacaggattt gattattgaa ggcttgagcg tcggcgttat
     8221 ccgccgctag tggaggattt atgcagttcc cgcaatcact gaaccgtaca caactgcctt
     8281 tgttcgatgc atttttggca tcgacggtga cgccattact tgccgatagc gctcagctgc
     8341 cttggcagga tgatgagaca gaggctttca gtgaactgtc gctgcgtggt gcagccgggc
     8401 actgcctgaa ccttctagcg ccgattctgc gtgaacttag cgaaaaccac gacgaacgct
     8461 ggttgaccct gatcgccccg ccgcccagcc ttacccaaac ttggctacgc gaagcgggcc
     8521 tgaaccgcga acgcattctg ctgctgcacc cgcgcggcgc acaaagcgcg ctggagttgg
     8581 cgcaacaggc gttgacgctg ggccgcagtc acacggttgt tagttggctg cacccgctgg
     8641 aacgccaagc gcggcaacag cttgagcaag cggcccgcca gggcaaggcg caatgcctga
     8701 atatcagcct ggggtaattg gccaaaggtc acgcacaagg aatgtgcgca tcagccagta
     8761 gaacctagtg cgagaaacag ccatcgcgag cagaacgctc tagctcagag cccggaagag
     8821 ttctgcaatc aatgcagaac ccgtggtcct tcttcctgct ggaaattatt ttcagccaag
     8881 cgccccgcca tctgcacacc gacattgagc atggccttgg ccacctcgac gtgctgcccc
     8941 tgcaggaagg ctttggcatc ggcagaaaaa tccagcacca ccagtgactc ttcatcctct
     9001 gcacgacgca gggcaatacg cccgtcgggc aattcaacaa tttccaggaa tgatgtaggc
     9061 attggcttag ctctccacgg taggccgaca gtgtaacagc cgacccgggc tagccctagt
     9121 ccgccggtcc aatcagcctc ttaccattcg ctcagggttt gccgaaagcg ctgtgccaga
     9181 gccttcagct gctgacgcca agcttcaagc gtctcctgcg ccaagggtgc gacttcttca
     9241 tccagactga ccgctgtgat caatggcgta gcgggatcaa ccttggcttt cttacccgcc
     9301 tgcggcggct gaaacagctc ggcataggcc ttgagcaact gagcaaccca gctgtcagcg
     9361 gactgtgcca gctcgaccag ctcacccaac tcctggcttg gcgccgcctg caacacttcc
     9421 tcggtgagaa agcgttcggc ctgcggcgcg gcgctgtcag gcaaacgata atagccagca
     9481 atctcatggc acaggcccag caacgcaccg tacagatgaa acaacgcagc ctcgcgctcg
     9541 gcctggatca aagccagggc attcatgctc tggccttgct cggctttgcg ccaggactcc
     9601 agcgctaggc ctgcgtagaa gattttctgg ttggtgcggg tatagagttc attggccatt
     9661 gcggcaccct ccacagcgga ttggataagc atacgcgtgc cggccaatgg ccgacacgcg
     9721 ctccgctaga tcagctcttg tcttcgactt tccacttacc gccatcaaag aacgcacgcc
     9781 agccagtcgg cttgccgtcg acctcggtct gtacgtactg ctccttggtc ttgcgactgt
     9841 agcggatcac cgctgcgcga ccttgcgggt ctttctttgg cgcatcacag aggaagtgat
     9901 acttgggatc gatctcgtca cgatggggga tcaattccaa caccagcggt gcgcgggttt
     9961 cacggttttt cgggaactgg ctggcagcca ggaacagacc cgaagcgccg tcgcgcagga
    10021 tgtaggtgtc gttgaccttc tcgcatttga gctccggcat cttcaccggg tccattttcg
    10081 gcggcgccgc ttcaccgctt ttcagcagtt tgcgggtgtt cttgcactca ctgttggtgc
    10141 agccgaagaa cttgccgaaa cggccggtct tgagctgcat ctggctgccg cacttgtcgc
    10201 aatccagact cgggccttcg tagcccttga tgcggtactg gccctgctcc acttcataac
    10261 cggaacaatc cgggttattg ccgcaaatat gcagcttgcg ggtttcatcg agcaggtagg
    10321 cgtccatcgc ggtcgagcag atcgggcagc ggtgcttgcc gagcagcacg cgcgactccg
    10381 actcaccctc gtcatcggcg gcaatttcgt cacccggcac caggttgacg gtggccttgc
    10441 agcgctcttt cggcggtagt gcatagccgg aacaaccgag gaatacacca gtggaggcag
    10501 tacgaatagt catcggccgg ccgcagtcac ggcaggcgat atcggtcagc gtcggctggt
    10561 tggcacgcat gccgccctcg cctgcttcag ctacttcaag cttcttcttg aagtcaccgt
    10621 agaactcgtc gagcacatgc ttccactcgc gctcgccctg ggccacatcg tccaggtgct
    10681 cttccatgcc tgcggtaaag ccgtagtcca tcaggttgga gaaactctcg gccagacgct
    10741 cggtgacgat gtcacccatc ttctccgagt agaaacggcg gttatgcagg gccacgtagc
    10801 cgcggtcctg gatggtgccg ataatcgctg cataggtcga cggacgaccg ataccgcgtt
    10861 tctccatttc cttgaccagg ctggcttccg aataacgcgc cggcggcttg gtgaaatgct
    10921 ggctggggtc gagtttgacc agcttgagca cttcaccctg attcatttcc ggcagcacat
    10981 cgtcgtcgcc cggcttgctc agctgcggca acacgcgggt gtaaccgtcg aacttgagaa
    11041 tacggccctt ggcacgcagc tcgaactgcg ccgccgtcac ggtaactgtg gtcgacaggt
    11101 attcagccgg cggcatctgg caggccacga actggcgcca gatcagctca tacaggcgct
    11161 cggcgtcacg ctccatgccc gacagttgag tcggcttgag gttgacgtcg gaaggacgga
    11221 tcgcttcgtg cgcttcttgg gcgccttcct tgctgctgta ggcgatcggg ttggcgggca
    11281 ggtacttctt gccgaactcg ccttcgataa agtcacgcac catgctcaca gcatcagccg
    11341 agagattggt cgagtcggta cgcatatatg tgatgtagcc tgcctcatac agacgctggg
    11401 ccatcatcat ggtcttcttc accccaaagc ccaggcggtt actcgcggcc tgctgcaggg
    11461 tggaggtgat aaaaggcgcc gaaggcttgc tgctggtcgg tttgtcttcg cgcttgctga
    11521 tgttgtagct ggaagctttc agcgtggcca gggccgccat ggcctgggcc tcgttcagcg
    11581 gcttgaaggc agcgccgttc tcacgggcta cttcaaagcg cacattggca cctttggcag
    11641 tgcccagatc agcgtgtact tcccagtatt cttcggggac gaacgcgcgg atctctttct
    11701 cgcgctccac caccagcttt accgctaccg actgcacacg accggcagac aggccacggg
    11761 cgatcttcgc ccacagcagc ggcgaaacca tataacccac tacgcggtcg aggaagcgcc
    11821 gcgcctgctg ggcattgact cgattgatat ccagctcacc aggcttggaa aacgcttcct
    11881 gaatcgcctt cttggtgatt tcgttgaaca ccacgcgttt gtaacggctg tcgtcgccac
    11941 cgatggattc gcgcaggtgc caggcaatgg cttccccctc gcggtccaag tcggttgcga
    12001 gatagatggt gtcggcatcc ttggccaaac ggcgcaattc ctcgaccacc ttctctttgc
    12061 cgggaaggat ctcgtacttg gctttccagc cgtgttcggg atcaaccccc atacgcgcaa
    12121 acagctgacg cttggctttt tccttgggcg acagcgccgg tacttcacct gcagcggctt
    12181 taccacgctt gaccggctct ttggcggtgc ttgccgaacc actggtgggt aggtcacgga
    12241 tgtggccgat gctcgacttc accacgtact cgttgcccaa gtacttgttg atggtcttgg
    12301 ccttggccgg ggattccacg atgaccagcg atttgcccat ggattggaaa att
//
LOCUS       DASRUM010000187        48956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_756991, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000187 DASRUM010000000
VERSION     DASRUM010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 48956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48956
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756991"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1375
                     /locus_tag="VFF22_11970"
     CDS             62..1375
                     /locus_tag="VFF22_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX17560.1"
                     /translation="MKTPLWFPQSFFARTLWLVLIVVLFSKALTLVYLMMNEDVLVDR
                     QYSHGAALTLRAYWAADPEDRATIAEAAGLKRVPYEEVPTTEQHWPYSEIFARQMQTE
                     LGPDTEVRVRAKSPPALWVHAPVLGDDWLRVPLYPHPLRGQKIWSVLGWFLAIGLLST
                     AAAWIFVSQLNAPLKRLVFAARQIGKGRSVRLPVSDTPNEMAEVYRAFNQMAEDVEQA
                     ARERELMLAGVSHDLRTPLTRLRLSMEFLHTDSELTEDMVRDIEDMDAILDQFLAFIR
                     DGRDEPLEQLDLLDLISEVVAPYNQKQEMVRLCLEPVPAFPLRRVSLKRLLVNLIENA
                     LRYGGNAVEVAVSLSGDYSAPYVVLSVLDRGAGIDPAELGSIFNPFIRGDRARGGQGA
                     GLGLAIVKRIAALHGGSVELRNRSGGGLEARVCLPLGLLLPRDAV"
     gene            1443..5228
                     /locus_tag="VFF22_11975"
     CDS             1443..5228
                     /locus_tag="VFF22_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZX17561.1"
                     /translation="MPAVPHRLSPWTWLLPLPLLHLATWLSLATQFADGAALWYPPFA
                     LALVFALWWGPRVLLAVYLNAMLSIPLWGLEWQWAPLYALPETLGVALAWVLLRLRPF
                     DPALPDLSHLLRFMLLGVLLPVIPVALGVQGNLWLLGPASLEEMQSGSMTLWLADCLT
                     ALAIAIPLLAYLTPWLRRRGWLAASVRSGCEQVPEALRQLPPWPLLVLLLIGMPWLLG
                     VLPLTLNLPLIGVMMLGLALIWGFAGGLCGALLSVLMVLILPLLRGLVNSTAWLDPQR
                     LELHFSVLLFMLATLLVGRSLSDLRQALARSAQMQQQLALANLALEASPLGVIIADAR
                     QPALPLIYCNPAFAQITGYSREETLGNNWNFLLRDDQQQQGLSRLQQAVRDGMGCDVV
                     LRNYRKDGQQFWNEFTLAPMRDDQGISHFVALQSDVTAREQLAIQVRTQSDELLKQSY
                     LFSQTEHIANLGGWVLNMPEQDMDWSAGCFRIYELDPKNGTPDLNGALSYLDASGRVL
                     AEQVLQQLIDGLEQFDIEVRLLTAKGKTRWLRIRGITERDGAELVRVYGALQDVSARK
                     YAEQQLRERDERLRLFFEAPLIGMALTSPNFAWEEVNHKLCSILGRSREQLLASSWEE
                     LSLEDDLQNELPLLDQVLSGSREGFELEKRFRRADDSLVHTRVSLRAVRHSTGRVRMF
                     LLLVEDISERLQAEARYRTIVENAPEAILLFDLNGTLVECNDNALRLLRYSREEIASK
                     RVFDLSPNKQADGQLTAELGKVYLKRAMNGEAPVFDWLHRDSKGRQIPCEVRLVRMPG
                     EPVLIRGSVTDISERQRYQREIERLAFSDELTGLPNRRLLLDRLQHAMDRELRENTLG
                     ALLFIDLDHFKTVNDSLGHLVGDGLLREVTTRLAGGLRAEDTLARMGGDEFVVLLEGL
                     DSNPQLAAEAAAVTAEKLLKSLVGTCWIDGHELSISASIGIALHPFGSQVAADVLKQA
                     DTAMYRAKQGGRNALHFFAPEMQAAIDQRLQMQSELRQAVQRNQLHLVFQPQLALVTE
                     RIAGAEVLLRWVHPERGEVMPGDFIPLAEETGLIEELGAWVLEQACAALARWLLQWPQ
                     LVLAVNLSPRELRQRSCVARISECLQRHQVPATALELEITEGVLLEEVEQCISNMQAL
                     KALGVRFAIDDFGTGYSSLTYLKRLPLDRLKIDRSFTWDMDGEDGSGLMLVQTILVIA
                     RNLGLECVAEGIESQWQLDCLRQHGCSLGQGYHFSRPLKEADFQAWMQQHRDPPPLL"
     gene            complement(5232..6137)
                     /gene="rimK"
                     /locus_tag="VFF22_11980"
     CDS             complement(5232..6137)
                     /gene="rimK"
                     /locus_tag="VFF22_11980"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HZX17562.1"
                     /translation="MKIAVLSRNPRLYSTRRLVEAGQLRGHEMVVIDTLRAYMNIASH
                     KPQIHYRGKPLEGFDAVIPRIGASVTFYGCAVLRQFEMMGVFPLNESVAIARSRDKLR
                     SLQLLSRRGIGLPVTGFAHSPDDIADLIDMVNGAPLVIKVLEGTQGIGVVLCETATAA
                     ESVIEAFMGLKQDIMVQEYIKEAGGADIRCFVVGDKVIAAMKRQAKPGEFRSNLHRGG
                     SASLIKITPEERMTAIRAAKVMGLSVAGVDILRSNHGPLVMEVNSSPGLEGIETTTGK
                     DVASLVIQHLEKNSGPNLTRTKGKG"
     gene            complement(6134..6568)
                     /locus_tag="VFF22_11985"
     CDS             complement(6134..6568)
                     /locus_tag="VFF22_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease"
                     /protein_id="HZX17563.1"
                     /translation="MREWINLPELGMIGLRAKIDTGASTSSLHASDIQPFDRDGEPWV
                     RFDAHLGTVVQRRHRCEAQLVSLKTIKSSNGHAQSRYVIRTNLTLGEREWPVEFTLTC
                     RKTMRYRVLLGSKALIDGQLLVNPALTYVQNKPTLADSPGAP"
     gene            6700..7131
                     /locus_tag="VFF22_11990"
     CDS             6700..7131
                     /locus_tag="VFF22_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HZX17564.1"
                     /translation="MSDKDDDKVRLDKWLWAARFYKTRALAKAAIEGGKVHHRGERCK
                     PGKEPRVGDVYLIRTGFEERSVVVQALSVVRRGAPEAQALYAETAESIERREQAAAQR
                     KAGALGMQTDGRPSKKQRRQMHDFNDRQDGGLRHPWADEEV"
     gene            complement(7128..7922)
                     /locus_tag="VFF22_11995"
     CDS             complement(7128..7922)
                     /locus_tag="VFF22_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZX17565.1"
                     /translation="MSPSFINQQWRIRPLVACHIVALLLLASWLWAPSRALWDQFDLQ
                     LFALLNEPVHSAGLWAQTWAIGSMRPVDMGVGVLMLAVMLKADLVFSGAQVRKALYAF
                     LVTLIVMLLFRVVFAELVKLMDWQRQSPSLVVEGTARLTELFPDWEERWNMKDSATRS
                     FPGDHASVLLIWAMFMSFFARNWRLLLVWSMAVIFMLPRLVAGAHWASDALVGGVFLA
                     LLGLAWGCFTPFAYKASEWLEKITLPLMRLLARLPLLGRLSVISGR"
     gene            8139..9026
                     /gene="hslO"
                     /locus_tag="VFF22_12000"
     CDS             8139..9026
                     /gene="hslO"
                     /locus_tag="VFF22_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HZX17566.1"
                     /translation="MSDFSQRFLFDDSDIRGELVGLTDSYSHVLAKHTYPQPVAQLLG
                     ELLAAASLLIGTLKFEGLLILQARSSGAVPLLMVECSSEGELRGIARYHAEQITADAN
                     LRELMPDGVLAMTVDPKSGQRYQGIVDLEGDTLADCLTNYFATSEQLPTRFWLSADGQ
                     RARGLLLQQLPADRLKEADEREASWQHAMALADTLKAEELLGLDNETLLHRLYHQEAL
                     RLFDPRALQFRCSCSRERSSRALISLGQADAEALVQEHGGSVEIDCQFCNERYLFDAA
                     DVLQLFIDGSIEAPSDTRH"
     gene            9189..10730
                     /locus_tag="VFF22_12005"
     CDS             9189..10730
                     /locus_tag="VFF22_12005"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HZX17567.1"
                     /translation="MMQANNAVYTDISVAQLVEEALRRGEGELAANGSLVVRTGHRTG
                     RSPADRFIVQEPSTEAKIAWGAINRPFPADKFDALWDRVQAFSDAQDSFVSYVHVGSA
                     EAHYLPVKMTTATAWQNLFGRQLFINPPQYNPADKGEWQVLNVANFECVPERDGTNSD
                     GCVILNFAAKKVLIAGMRYAGEMKKAMFSVQNFLLPDADVLPMHCAANIGEEGDVTLF
                     FGLSGTGKTTLSADESRYLIGDDEHGWGVGVVFNIEGGCYAKCIDLSEKNEPVIWKAI
                     QFGAVLENVVLDENRVPNYADDSLTQNSRAAYPLELVEKRSEKNLGGEPNAVIFLTCD
                     LTGVLPPVSILNEEQAAYHFLSGYTALVGSTEMGSGGGIKSTFSTCFGAPFFPRPAGE
                     YAELLIKRIRGFGSKVYLVNTGWTGGGYGVGKRFNIPTTRGVIAAIQSGALIGTETEL
                     LPIINLSVPKSVPGVETNLLNPRNTWADKNAYDEAAKGLAKLFIENFQKFDVSDAIKN
                     AGPQL"
     gene            11629..13551
                     /locus_tag="VFF22_12010"
     CDS             11629..13551
                     /locus_tag="VFF22_12010"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HZX17568.1"
                     /translation="MSTLERVFGFKQLRPGQERVVTAVLAGRSAAAIFPTGSGKSLCY
                     QLPALHLPHLTLVVSPLLALMQDQLAFLHARGISAASIDSAQSREEASAVMARAKSGE
                     LKILMISVERLKNERFRNFIAQVPISLLVIDEAHCISEWGHNFRPDYLKLPDYQRQFG
                     IPQVLLLTATATPPVIADMQQKFAIAAEDVVTTGFYRANLNLLVEPVSGADKQRRLEQ
                     WLGAKTGQPSIVYVTQQKTAEQVAARLSQRGIAASAYHAGMPHEAREAIQRQFMAGQI
                     NCIVATIAFGMGIDKADIRNVVHFDLPKSIENYSQEIGRAGRDGQPSDCLVLANRDSL
                     NVLENFVYGDTPELEGIRSVLDKLLSESDNGEWELMLNQLSEQSNIRQLPLKTLLVQL
                     ELRGIIAPRYAYFAEYRFKFLLEPEALLAKFEGERRQFVEALISTSQRARTWCTVDFE
                     QLYQQHRAERGRVIKALDYFQEKGWIELESKQMTEVYALLDPGFDPVALSGELHGYFS
                     QQEASEIRRIHAMLALFASEQFLSQRLAAYFGDANAPQQCGHCSVCRGQVARLPAPPA
                     LPPLAQQDAHGLCDGLVRKHQELKGSEPSAECLTRFLCGISVPLFTKLKARQLGGFAA
                     LEDYPYAEVREWLRGA"
     gene            13696..14211
                     /locus_tag="VFF22_12015"
     CDS             13696..14211
                     /locus_tag="VFF22_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZX17569.1"
                     /translation="MPNDRRAWVPGGTYFFTVNLQDRESDLLVREIALLRRVVVTTKA
                     RHPFAINAWVVLPEHMHCLWTLPAGDSDFARRWMVIKQLFCRGLPAATGANARRERGI
                     WQRRYWEHLIRDSQDWRAHFDYIHINPVKHGWVSRIQDWPYSSFHRAVRDGLYPQDWA
                     GNVDGVVPGGD"
     gene            complement(14324..14455)
                     /locus_tag="VFF22_12020"
                     /pseudo
     CDS             complement(14324..14455)
                     /locus_tag="VFF22_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
     gene            complement(14496..15107)
                     /locus_tag="VFF22_12025"
     CDS             complement(14496..15107)
                     /locus_tag="VFF22_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HZX17570.1"
                     /translation="MSRLRLLAALLLIGSLAACSVLPKSQELSIYRLPASSLPSHDAS
                     ADWALRVNKPYSSQLLDSTRIAVLPPGDQISAYQGVRWSDRAPLLLRDRLIDAFLDDG
                     RLKAVSSDESRLQADLELDGDLRAFHSEYQDGRPAARILFEARLVQSGSLRILASRRF
                     EVSQPANDTSVPAVVNAFGQAGDQLAREVLEWTLKQGQTAQLQ"
     gene            complement(15104..16021)
                     /locus_tag="VFF22_12030"
     CDS             complement(15104..16021)
                     /locus_tag="VFF22_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019580311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZX17571.1"
                     /translation="MEPRAHHVLIGLFTVLMVAAALGFALWLSKASSDQELRDYDVIF
                     NEAVSGLSQGSAVQYSGIKVGDVISLRLDPNDPRKVRAHIRVNADTPIKEDTRARLSI
                     TGITGAALIQLHSGSPESPVLVSKGEQHAEIIADRSPLSRLMSNGEDLVLSVTRLLNR
                     ANQLFSRDNIGRITRTLENIEQTSASVAEQRDELRTALQQMSTASQEAADLMRNANQL
                     LSGQGREAMDSASRLMASLERSSSNVERLLTDNRAALDGGMQGIGELGPTIIELRETL
                     SALRSFARRLEEDPAGYLLRSDTIKEFQP"
     gene            complement(16022..16810)
                     /locus_tag="VFF22_12035"
     CDS             complement(16022..16810)
                     /locus_tag="VFF22_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZX17572.1"
                     /translation="MKGEVIIEVRNLVNRFGAQTVHQDLALDLYRGEVLGVVGGSGTG
                     KSVLLRSILGLRRANSGTVRVFGEELLSLPPEQRSQLERRFGVLYQRGALFSSLTVTE
                     NIALPLLEHAGLKRPAAERLAQVKLALVGLPREAGDKYPTELSGGMIKRAALARALAL
                     EPDILFLDEPTAGLDPIGAAAFDQLILTLRDALGLSVFLVTHDLDTLYTICDRVAVLA
                     EKRVLVADRLDVVAATDNPWIHDYFHGPRGRAAEQAATGAPGSN"
     gene            complement(16807..17952)
                     /locus_tag="VFF22_12040"
     CDS             complement(16807..17952)
                     /locus_tag="VFF22_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX17573.1"
                     /translation="MSTAPFIKPLESSRTDAPSGLRIGGDWTLPHYASLEVEVLALRG
                     TLNSAANIDLRELGALDTAGAALLVELLGSQRLAELANDAPDLASERRALLQAVGNAI
                     ADAGDALPSKRIPAWREFFGQIGQAVEELWQQGVALLGFIGMTLAAMFAIILRPGRWR
                     ITSLVAHLQQSGLNAVPIVALLTFLVGAVVAFLGATVLADFGASIYTVDLVAFSFLRE
                     FGVLLTAILMAGRTASAFTAQIGSMKANEEIDAIRTLGLNPLELLVLPRVFALLIALP
                     ILTFIAMLSGILGGAVVCALSLDISPAMYLAMLQENIALKHFVVGMAKAPIFAFLIAV
                     IGCLEGFKVTGSAQSVGERTTSSVVQSIFVVIVLDALAALFLMEMGW"
     gene            complement(18012..18938)
                     /locus_tag="VFF22_12045"
     CDS             complement(18012..18938)
                     /locus_tag="VFF22_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HZX17574.1"
                     /translation="MSSLYRLVTRSDFDGLVCAALLKHLNLIDDILFVHPKDMQDGKV
                     AITSNDITTNLPYVPDCYLAFDHHLSETIRNQPASNHLIDADAPSAARVVWKHYGGHE
                     AFPREWDEMMEAVDKADAAQFSRDEILDPHGWELLSFIMDARTGLGRFRDFRISNYQL
                     MMQLIDFCQTHGIDEILELPDVDERVALYLEHQREAKAQIQRCATVYHNLVVLDLRNE
                     ETIYAVNRFVIYALYPQCNISIHVMWGVKQQNTVFAIGKSILDRSSNTNVGELCLSYN
                     GGGHANAGTCQVANEDAEAALRELIAEITADG"
     gene            19130..19471
                     /locus_tag="VFF22_12050"
     CDS             19130..19471
                     /locus_tag="VFF22_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZX17575.1"
                     /translation="MSPITVLRDTQPIPLLDACKWTRIAGEPHTVNLNAYTSADGSKI
                     MGTWICTPGKWQVAYEKWEYCHFQEGYCIITPEGQAPIHLRAGDIFIVEPGMKGTWEV
                     VETVRKYFVFA"
     gene            complement(19483..20136)
                     /locus_tag="VFF22_12055"
     CDS             complement(19483..20136)
                     /locus_tag="VFF22_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HZX17576.1"
                     /translation="MTLKTLGLALSTTLLLAGCASKTTQPSQYSGFLSDYSQLQPAQS
                     ASGAPVMRWVSPDFKAQNYRSVLVEKPVFYPAPTPSEQVSQKTLDEITSYLQQAMQLE
                     LAGQYRVVQQADQDTLVLRSAITAVNLSAEGLKVYEVVPIALLAAAASTAAGTRDLNT
                     NIYVELEAIDGRNSQPMARVVRKGHGLQLENDSTQLSLKDIKPALDVWAKDARNFKP"
     gene            complement(20235..21173)
                     /locus_tag="VFF22_12060"
     CDS             complement(20235..21173)
                     /locus_tag="VFF22_12060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007965946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX17577.1"
                     /translation="MTPDALATLQQHLLTALNSAPNETRRLFHGRGRCWPGLEQVTVD
                     WLQGVLLVALFKPVSDAELAALQQMLLDIGQSAAWQQSGAHSLLLQQRYLPESPTKWL
                     LGEPVEHWDICEHDLQYRLDLGKKQNSGLFLDMRYGRQWVQAQAAGKRVLNLFAYTCG
                     FSVAALAGGARQVVNLDMARAALSRGRDNHRLNQHDLSQVVFLGHDLFKSWGKVAKYG
                     PYDLIIIDPPSFQRGSFVLSKDYPKVLRRLPELLNANGVVLACSNEPEIGPDYLIQAM
                     ASEAPGLRFSERLQNPPEFPDSQPESALKALVFCRD"
     gene            21325..21927
                     /locus_tag="VFF22_12065"
     CDS             21325..21927
                     /locus_tag="VFF22_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZX17578.1"
                     /translation="MHIDEAIRSRRAVKGYDPAFILSDAEKDELLQLALLAPSAFNLQ
                     HVRMVEVSDPALRQQLREVAWGQAQVTDASMLVVVCAKLDSWQEDVGRVWDGAPAEVQ
                     AYMAGAIDTYYRDKPQVQRDETMRSCGLMAQTLMLAARGKGLDSCPMDGFDFDAVAKL
                     IKLPANHVIALMVAVGKKTIEPKPRVGKLPFNEVVLRNGF"
     gene            22095..23585
                     /locus_tag="VFF22_12070"
     CDS             22095..23585
                     /locus_tag="VFF22_12070"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZX17579.1"
                     /translation="MSAEHLDVLIIGAGLSGIGAAYHLMKHCPGKRFAILEGRAALGG
                     TWDLFRYPGIRSDSDMFTLGYNFKPWTNPLAIADGPSILSYIEETAEENGIQEKIRFQ
                     HKVLKADWCSEHATWTLQVQRGDDPEPIKLSCQHLLMCTGYYRYEAGYTPDFKGREQF
                     QGEFIHPQLWPENLDYSDKNVVVIGSGATAVTLVPAMTDKAKHVTMLQRSPTYVVSLP
                     QGDPISAFLRRFLPETWVYRLARTRNVAVQLGFYKLSKAFPSLVRKALLGMAKRQLGA
                     NFDMRHFSPRYKPWDERVCAVPNGDLFKVLRKGKASVVTEHIDGFTAKGVRLKSGQVL
                     PADVIISATGLELQLFGGMQVAVDGVSFDAAKSMGYRGIMLRDLPNAAVVLGYTNASW
                     TLKADLSSEYFCRLINHMDANGMRQCTPRDTARQVKNAPFLDLASGYIKRAADKIPSQ
                     GDRAPWKLYQNYLLDLALLRYGKVEDGYLEFSSPHVGESAATATAV"
     gene            23767..23937
                     /locus_tag="VFF22_12075"
     CDS             23767..23937
                     /locus_tag="VFF22_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17580.1"
                     /translation="MESLQQEQRYLPELRAHIDKLLAKGWVIAERSPLTLQSGRKTYM
                     VVHGMLIGDNAL"
     gene            complement(24007..26373)
                     /locus_tag="VFF22_12080"
     CDS             complement(24007..26373)
                     /locus_tag="VFF22_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZX17581.1"
                     /translation="MTLRGKILGFFLLLLLAVMVLLLALVYRSTYQHTLSQVAGQLNF
                     AHAVLSNELQSRRQAQSAMADLIAKDFTLLGEIADLIASPQQEAQSLSAALESFASRS
                     QASFALVSAPEGLILAGTSGELLPGAALPWDDLLDEEDPQSAERLVVFGERVLHINAT
                     PIFAPRPNLMGWLLMAFELDDQATAHLARLSGADVALIDGSPGQYRVLASNLQALPRA
                     ELAGQLLTNAEGVFRFELQGLEQVALRAPLIGTSSELQVLLMRSLSAELADYQPLQWQ
                     LATIFAIALLLAGLGALWIANTVSRPLSQMVDNVRRIASGDYQVAMQVPATQASGEIG
                     LLAREFVAMQQGIAERETTISFLAYRDPLTELANRNHFVAELQQALAHSSPGEGVAVL
                     LMDLDNFKDLNDTLGHDAGDQLLCQLAARLQALLRPGEHLARLGGDEFALLISRCQPG
                     QVIPAAEHYRAALREPFAVRGISMTLNATLGIALYPDHGESAGSLLQHAEVAMYLGKA
                     QRSPYALYRPELDRHSLLRLALMSELKGAVEDGQLSLYFQPKLNIRARSLYGVECLVR
                     WIHPVHGFIPPDEFIPLAEQTGNVCALTRWVVRTAVAQSRAWQEQGLELKTAINISAL
                     DLADPGFAGFIAAQLAEHGVPPSSLIVEITESAVMADPELAMGQLQELCELGVQLSID
                     DYGTGYSSMAQLKRLPVHELKIDKSFVLDLLSNPDDDIIVRSTIELGHNMGLKIVAEG
                     VETEAILERLGQLGCDIAQGYLLSKPLPPAAFADWLAATHWQPPRHTG"
     gene            complement(26370..27014)
                     /locus_tag="VFF22_12085"
     CDS             complement(26370..27014)
                     /locus_tag="VFF22_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine utilization protein"
                     /protein_id="HZX17582.1"
                     /translation="MQKSALALLLALLGTLAQAQTLEIVLLDNQGKPLSDAVFWVEPG
                     PAQNQPATALMDQQKRQFTPYILPVQVGTTVNFPNSDPINHHVYSFSPAKRFELRLHQ
                     QKAAPQEMRFDQPGLVTLGCNIHDWMLGFILVLDTPWFAQTDSQGKTRLDFDATAGQT
                     LHIWHPRIADPQEALNRAVPSDGTLTWQLTGTLKRDPRPKTPAMRSQGKGGYAP"
     gene            complement(27083..27982)
                     /locus_tag="VFF22_12090"
     CDS             complement(27083..27982)
                     /locus_tag="VFF22_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX17583.1"
                     /translation="MNISTFDLNLLRVFDALMREQNVSRAAERLSLSQPAVSNALNRL
                     RELLDDPLLVRVGRSMQPTPRAQALETPIRAALQQIEQSLSAGEVFDPASSRQHFSIA
                     VTDYVELICMPRLLKQLSQQAPGMRIDIRHLSPSLPAEALDKGELDLVLGRFEDIPAR
                     FARQHWMSETLQLALRRDHPLVSGPMDLPTFLQLRHLWVHGGQTRGMVDQWLGEQGLC
                     REILYTTPNYLQAAHIVSSSELAAVLPTQLARHFASLLPLQLFELPFALGPFHLEIVS
                     LAQRQRDPALHWLIEQIQQIAQR"
     gene            28083..29351
                     /locus_tag="VFF22_12095"
     CDS             28083..29351
                     /locus_tag="VFF22_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZX17584.1"
                     /translation="MSKAFEVQQAAVIGAGTMGRGIVMSLANAGIQVLWLDNNPQMLE
                     QAMTVVAETNAHNLKLGRISEEQSAARLACVTPVGEYAALADADLVIEAVYENLELKQ
                     QIFRTLDATLKPGAILASNTSALDIDAIAAVTARPQDVLGLHFFSPAHIMKLLEIVRG
                     AKTAPAVLDAAVALGQRIGKVSVVAGNCDGFIGNRMLHTYVREARMLLLEGAYPHQVD
                     AALQGFGFAMGPFRMYDVVGIDLEWRARELAGKGQDDPAVQVDNRLCGLGRFGQKVRM
                     GYYRYAEGSRQAEHDPEVDALVLQVSEQLGFVRREIGPEEILERCLLALVNEGAKILE
                     ENIAANSHDIDLVYLNGYGFPADKGGPMSWADSQGVAAIHDRLKALQAAFGEHWLPAR
                     LIEQLAASGQRFADVRVSDMRVSDVQEGRV"
     gene            29348..31138
                     /locus_tag="VFF22_12100"
     CDS             29348..31138
                     /locus_tag="VFF22_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HZX17585.1"
                     /translation="MSYQAPLRDMRFVLHELFDISRHCELLGNGLDRELIDGVLEEGA
                     RYAAEVVAPLNRHSDEQGCQLNDGQVRTPDGFAEAYQQYVDNGWASMTGPSEYGGQAL
                     PQMLACNFHEMLMGASLSFRVYSGLTEGAVLALYKHGSESLKNTYLHKLVSGQWAGTM
                     CLTEPQAGTDLALLRTKAEPQADGCFKVSGSKIFISGGEQDLSENIIHLVLARLPDAP
                     AGVKGISLLLVPKFIAAADGTPGERNALSCGAIEHKMGIKGASTCVMNFDGATGWLIG
                     EANQGLACMFTMMNDARFQVGLQGLGIGEAAFQGALRYARERLQSRALSGPAEPGKAA
                     DPIIVHPDVRRMLLTQKSLVEGSRMLAAYCARQLDLEHGNPDAAARKAAGKRAALLIP
                     IVKAFFTDMGQEVASHAVQVYGGHGFIREWGIEQLMRDSRITQLYEGTNGIQALDYIR
                     RKVLGDGGAELSALQAEFSELCDAQVRRPALAALAQTVQARLGEWRELSSEVIAASQR
                     DVQEIGAVSVDFLQYSAYVLLAGFWLQAAARAQDALEAGSGEAAFYQAKLQSADFYLR
                     RVLPRASAHRESLLGGADCLMAMDEASFAF"
     gene            31209..32372
                     /locus_tag="VFF22_12105"
     CDS             31209..32372
                     /locus_tag="VFF22_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZX17586.1"
                     /translation="MLPFSFATTAQILCEIGASQRLAELCRERGAQRVLIVTDPGISK
                     LGLLADVLPGFARTELTVEVFDQVVADPPEAILLAAVEQARALKAQLIVGFGGGSSMD
                     VAKLVALLAHPECGQELAQIYGVGNARGQRLPLILVPTTAGTGSEVTQISIITTGETT
                     KMGVVSPLLLPDLAVLDAELTLGLPAAVTAATGIDAMVHAIEAYTSALKKNPLSDLLA
                     REALRLLAANLDKVVHDGANREARQAMLLGSCLAGQAFANAPVAAVHALAYPLGGHFH
                     IPHGLSNALVLPHVLGFNANSAAPLYAELAPLVLGEQLRAGSELQQTEQFIGAMADFS
                     VRSGLPTRLRDAGVPQEMLPTLARDAMQQQRLLVNNPREMTEAHALAIYQVAY"
     gene            32401..32844
                     /locus_tag="VFF22_12110"
     CDS             32401..32844
                     /locus_tag="VFF22_12110"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZX17587.1"
                     /translation="MSQPQHLRSDYRHFQPITTRWHDNDVYGHVNNVTYYSFFDSAVN
                     SYLIEVGGLDIHNGEVVGFVVSSSCDYFASIAFPERIEIGLRVGKLGNSSVQYELAVF
                     KADEDEACAAGRFVHVFVDRSSNRPVSIPENLRAALTVLLMEPAL"
     gene            complement(32916..33350)
                     /locus_tag="VFF22_12115"
     CDS             complement(32916..33350)
                     /locus_tag="VFF22_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007971259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HZX17588.1"
                     /translation="MRTLFSALVFSALVSPLALAGDTGKVAIGGGVGGALGNVIGQQV
                     GGSTGAAIGAGLGGAAGGVITARDGNKTEAALGGGLGAAGGSVLGNKVGGSTGSTIGA
                     GLGGAAGGALANEYAGGSERDRDHYHGKKHKKHKKHKKHKHH"
     gene            complement(33497..35818)
                     /locus_tag="VFF22_12120"
     CDS             complement(33497..35818)
                     /locus_tag="VFF22_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HZX17589.1"
                     /translation="MSLQPINPRYQPYSGPAAGWGALRSVTRFWLDSKQPFKNLRALL
                     KTNQHGGFDCPGCAWGDSPEDGRIKFCENGAKAVNWEATKRRVDAAFFARYSVSQLRE
                     QSDYWLEYQGRLTEPMRYDAASDHYRPISWDAAFALIAEHLQALESPNQAEFYTSGRA
                     SNEAAYLYQLFVRAFGSNNFPDCSNMCHEASGVALGQSVGVGKGSVTFADFEHSDAIF
                     VLGQNPGTNHPRMLEPLREAVKRGAQVVCFNPLKERGLERFQHPQHALEMLSNGSEPL
                     NTAFFRPALGGDLAALRGMAKFLLQWHREALAQGEPAVFDLEFIAEHTDGVDDYLALL
                     DASSWESLVEQSGLSLDELQHAALLYRRAERVIICWAMGITQHHNSVATIQEIVNLQL
                     LRGNLGRPGAGLCPVRGHSNVQGDRTMGINERPSAAFLDALEQRFNFQVPRVQGHNTV
                     EAINAMLDGQAKVFIGLGGNFAQATPDSPRSHAALSRCALTVQISTKLNRSHLTTGTD
                     ALILPCLGRTDIDHQAGGPQAVTVEDSFSMIHASYGQLEPLSQQMRSEPAIIAGIAKA
                     TLGNHPVDWDALIADYRRIRELIAETISGFNDFNQRLQHPGGFYLGNAAAERRWLTAS
                     HKANFKANPLPADLLPTQVRSTGQTPDLILQTLRSHDQYNTTIYGLDDRYRGVKGQRQ
                     VLFVNEADILRLGFQPGQQVDIQSIWDDGIERKVVGFTLLAFDIPAGQAAAYYPEANP
                     LVPLQSFGTGSFTPTSKYVAIRLQAHQAEARIL"
     gene            complement(35815..36657)
                     /gene="fdhD"
                     /locus_tag="VFF22_12125"
     CDS             complement(35815..36657)
                     /gene="fdhD"
                     /locus_tag="VFF22_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HZX17590.1"
                     /translation="MPRHAPAIVKDAAPVLLPAPTYSYVELAEAQLDGAAALATETAL
                     AISYNGISHSVMMVSPSDLEDFIYGFSLSAGLIDSFDEIYDIRLQQHDSAISAEVQVS
                     NRAFWALKQQRRTLAGNSGCGLCGVEALEQALPQLQRLTPSPLPAAVHLQGLRQRIQT
                     VQTMARHSGAVHAALFIDGQGQIRLCREDIGRHNALDKLLGALHREGENLRQGFAVVT
                     SRCSLELIHKALRAGIGTLVSLSAPTTLSVQWARQHNLNLIHLPQQSAPRVYSPAAPG
                     VAQP"
     gene            complement(36777..37670)
                     /locus_tag="VFF22_12130"
     CDS             complement(36777..37670)
                     /locus_tag="VFF22_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX17591.1"
                     /translation="MDIKQLKFLIALDETRHFGQAAARCHITQPTLSMRLRQLEDELG
                     LELVRRGQRFEGFSAEGERILAWARSLMAAHDGLYAEAAACRGQLVGTLRLGVVPLAG
                     FDPMRLVQFFAEQHAGLRFQLFALSSEEILERLGRNQLDLGLSYLDRLNPEHFDSLEL
                     AETRMGLLYDRRHFQFTRPALRWESLIDLPLGLLSAGMHFRQSIDHSFRSRGLTPSPR
                     LETDAVHQLLQAVSAGLCCAIMPLNSGLAEFTKHLALTPIEDAHTLAPLGLIMRRSAP
                     RSALTEACFAEAHKLLPAAAS"
     gene            37864..38304
                     /gene="lysM"
                     /locus_tag="VFF22_12135"
     CDS             37864..38304
                     /gene="lysM"
                     /locus_tag="VFF22_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HZX17592.1"
                     /translation="MSLFAFVKEAGTKLWESLVGQEAQAAESLTAHVAKVGLGNPNIQ
                     ISVEGDKVTARGEVASQEEKEKILLALGNVAGVASVEDHISVTTPAPAARFVTVKKGD
                     TLSAIAKAEYGNANAYQKIFEANKPMLSHPDKIYPGQVLRIPEE"
     gene            complement(38347..39009)
                     /gene="yrfG"
                     /locus_tag="VFF22_12140"
     CDS             complement(38347..39009)
                     /gene="yrfG"
                     /locus_tag="VFF22_12140"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HZX17593.1"
                     /translation="MPSLPWNAIDTVLLDMDGTLLDLHFDNHFWLQHLPQRYAEHHGV
                     SRALAEAELLPLFREHAGTLNWYCTDFWSRELKLSIRDLKREVAHLIALRPDADTFIQ
                     ALRQAGKQVVLITNAHRDSLSLKLERIELAPYFDRLISSHDYGFPKEDQQFWHALQQD
                     ISFEPARSLFIDDSLPILRSAGRYGVGHLLAVRQPDSQAAPKDTEEFAAVEDYRELLQ
                     GL"
     gene            39143..39709
                     /gene="nudE"
                     /locus_tag="VFF22_12145"
     CDS             39143..39709
                     /gene="nudE"
                     /locus_tag="VFF22_12145"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HZX17594.1"
                     /translation="MRQKPTVLAREIVASSRLFRVEELQLRFSNGVERTYERLVGKGA
                     GYGAVMVVAMIDDEHALLVEEYCGGTDDYQLSLPKGLIEPGEDVLEAANRELKEEAGF
                     GAQHLELLAELSLSPGYMSQKIQVVLASGLYEERLPGDEPEPMRVDQTSLRELSSLIQ
                     HPQFTEGRALAALYLARDVLTQRGIFKP"
     gene            39706..40527
                     /gene="cysQ"
                     /locus_tag="VFF22_12150"
     CDS             39706..40527
                     /gene="cysQ"
                     /locus_tag="VFF22_12150"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HZX17595.1"
                     /translation="MSHPLIPAVIELVRQAGAATLPFWRADVTVNEKADASPVTAADM
                     AAHHILNDGLLALAPDIPVLSEEAADIALSERGQWSRWWLVDPLDGTKEFIAGSEEFT
                     VNVALIEQGRVVFGVVGIPATGLCYYGGAGLGAWRADAAGVSEEIRVRLAPQEAFTLV
                     ASRRHSSPAQEALLAGLSERFGDLQLASVGSSLKFCLLAEGNADCYPRLAPTSQWDTA
                     AAQGVLEGAGGEVLTLKGEALTYEARESLLNPYFLALPAAAAWRAELIQLARSLD"
     gene            complement(40671..42581)
                     /locus_tag="VFF22_12155"
     CDS             complement(40671..42581)
                     /locus_tag="VFF22_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HZX17596.1"
                     /translation="MTSRLPVIVGFGGYNAAGRSSFHHGFRRTVIESMDTSARQQTLA
                     GLAVMMKLVKVVDGDYQDDAGNTLSLAEIDSRFAEQILASTLVRRIEKQHLDVDAAHW
                     QKTIDISATAGQPLSFITLRKHLPDPLPSDWTVDELNASEVLVTLHDNCEFKVDSYRA
                     LPVKSAGQLPTGFEPSELYNARFHPRGLAMTIVGVTDALRATGIDWQTIMQHVAPDEV
                     AVFSSCIMSQLDENGFGGLMQSRLKGGRVTAKQLALGLNTMSADFINAYVLGSVGTTG
                     AITGACATFLYNLQKGIEQIANGKARVVIIGSSEAPINQECIEGYGAMGALATEDGLR
                     QIEGKETVDFRRASRPFGENCGFTLAEACQFVVLMDDELALELGADIHGAVPDVFINA
                     DGFKKSISAPGPGNYLTVAKAVASAVQLLGIDAVRERSFVHAHGSSTPANRVTESEIL
                     DRVASAFAIEQWPVTAVKAFVGHSLATASGDQLISALGSFKYGIIPGIKTIDGVAEDV
                     HQQHLSFATQDRSRASEQLDLCVLNSKGFGGNNASALLLAPHVAERMLRKRHGEAAFA
                     AYLQRREVTRKAAQAYDAQALLGDLGIIYNFGNDLIDDKGIEISSEQIKVPGFAQPLV
                     FKKDERYGDMLE"
     gene            42825..44474
                     /locus_tag="VFF22_12160"
     CDS             42825..44474
                     /locus_tag="VFF22_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HZX17597.1"
                     /translation="MHSVNPYEYGMPRDAANFQALSPLSFLERAASVYPQRLALINGR
                     LRQTWGETYSRSIRLASALAQRGIGLGDTVAVIAPNGPAMFEAHFGVPMCGAVLNAIN
                     TRLDAEAIAFILQHGEAKVLLVDKEFGELAQRVVGHLPQPPLVIGIDDTEYAEGLLIG
                     QLDYEALLAEGDPDYAWQLPADEWQAISLNYTSGTTGNPKGVVYHHRGAHLNALSNAM
                     CWDMSRFPVYLWTLPMFHCNGWCFPWALAAYVGVNVCLRHVRAEAIYPAIAEHGVDHF
                     CGAPIVLNMLANADEALKALKTQPVKVLTAGAAPPAAVIEAMEALEFRVTHVYGLTET
                     YGPSVACEWKSEWDSESPEQRARLKSRQGVRAPLLDGLMVADSDTLQPVPKDGQTIGE
                     IMMRGNVVMKGYLKNPSATAEAFAGGWFHSGDLAVWHADGYVEIKDRSKDIIISGGEN
                     ISSIEVEDVLYRHPLILEAAVVAMAHAKWGETPCAFVTLKPGAELTAEEVIAFCQQRM
                     ARFKVPGCVVFTSLPKTSTGKVQKFVLREQAKQLAAQQAVA"
     gene            44587..45588
                     /locus_tag="VFF22_12165"
     CDS             44587..45588
                     /locus_tag="VFF22_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZX17598.1"
                     /translation="MQVSKGLFAALSLTALASTAQAADEVVVYSSRIDELIKPVFDAY
                     TAKTGVKIKFITDKEAPLIARLKAEGANTPADLLITVDAGNLWQAEQEGVLQPTKSEL
                     ITANIPAQYRSSTDSWTGLSLRARTIFYSTERVKPSELSTYEALADKNWEGRLCLRTS
                     KKVYNQSLTATLIETHGAAKTEEIVKGWVNNLATDVFADDTALLQAIDAGQCDVGITN
                     TYYYGRLHQQQPDLKVKPYWPNQNDRGVHVNLAGAGVTKHAPHAEAARKLLEWMTTAE
                     AQTIFAGVNQEFPANPAVEPSAEVAAWGTFKADTIATEVAGKRQAEATMLMDRAGWN"
     gene            45826..47442
                     /locus_tag="VFF22_12170"
     CDS             45826..47442
                     /locus_tag="VFF22_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007940366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZX17599.1"
                     /translation="MAHPAQRRWYPIAIAVALLVLLPLSVLMFSWHEVDQQIWAHLWQ
                     TQLPRLLGNTLVLVLGVGIGVTLLGVSLAWLTSLCEFPGRRWLDWALMLPFAIPAYVL
                     AFVFVGLLDFAGPLQTLLREWFGSDVRFPRVRSTGGVIIVLVLVFYPYVYLLARNAFL
                     AQGKGLMEAARVLGLSPWRAFWRVALPMARPAIGAGLALAIMETLADFGAVSVFNFDT
                     FTTAIYKTWYSFYSLTSATQLASLLLLAVMLVLYGERRARGAVRPVNERARSKALYHL
                     KGGKALAASTWCGLVFACGFVIPVLQLIVWFWQRGRFDLDERYSALILHTLYLGAMAA
                     LITVSVALLLAFARRMAPTRLMRSTVGVANLGYALPGSMLAVAIMLAFSYLDRELVIP
                     LSSWLGGAGKPVLLGSLSALLLAYMIRFMAVAYGPLENSLARIRPSLPEASRSLGVGG
                     VGLFFKVYLPLLVPGALSAALLVFVDVLKEMPATLLMRPFGWDTLSVRVFEMTSEGEW
                     ARAALPALTLVLVGLLPVILLIRRSARRHG"
     gene            47615..48697
                     /gene="gcvT"
                     /locus_tag="VFF22_12175"
     CDS             47615..48697
                     /gene="gcvT"
                     /locus_tag="VFF22_12175"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZX17600.1"
                     /translation="MGQRTPLYDLHLALGAKMVDFGGWDMPLHYGSQVEEHHQVRRDC
                     GVFDVSHMTVVDVSGSQAKAYLQHLLANDIERLQSNGKALYSGMLNEQGGVVDDLIVY
                     LTDFGYRVVVNASTRDKDLAWMRLQSVDFDVQMQERAELAMLAIQGPHARSKTAELVS
                     QSRANLIQSLKPFEGQPDGDWFIARTGYTGEDGLEIMLPASEVVAFLNELVGAGISPI
                     GLGARDTLRLEAGMNLYGQDMDESVTPLAANMAWTIAWEPASRGFVGRAALQKQKAEG
                     VGSKLVGLVLEERGVLRAHQVVRVEGVGEGEITSGSFSPTLGKSIALARVPAATTERA
                     EVEIRGKWYPVRVVKPTFVRNGKALI"
     gene            48819..>48956
                     /locus_tag="VFF22_12180"
     CDS             48819..>48956
                     /locus_tag="VFF22_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HZX17601.1"
                     /translation="MSDEERHMSNIPADLRYAASHEWARLEADGSVTVGISDHAQEAL
                     GD"
BASE COUNT         8919 a        14525 c        15809 g         9703 t
ORIGIN      
        1 gcccgcgatc cgcaatccat tcgcgggcat ggcccgctcc tacaaaaccc tgtaagtgcc
       61 tatgaaaacc cctctctggt tcccgcaaag tttcttcgcc cgcacgctgt ggctggtgtt
      121 gatcgtggtg ctgttctcca aggcattgac cctggtttat ctgatgatga acgaggacgt
      181 gctggtcgac cgtcaataca gtcacggcgc ggcgctgacc ctgcgcgcct attgggcggc
      241 ggatccggaa gaccgcgcca ccatcgccga agcggccggg ctcaagcgcg taccgtatga
      301 ggaggtgccc accaccgagc agcattggcc gtatagcgag atttttgccc ggcagatgca
      361 gaccgagctg ggcccggata ccgaggtgcg ggtgcgcgcc aagagcccgc cggcgctttg
      421 ggtacatgcg ccagtgctgg gcgacgactg gctgcgtgtg ccgctgtatc cgcatccact
      481 gcgtgggcag aagatctgga gcgtgctggg ctggttcctg gccattggct tgctgtcgac
      541 ggcggcggcg tggattttcg tgagccagct caatgccccg ctcaaacgcc tggtctttgc
      601 cgcccggcag attggtaagg gccgtagcgt gcgtctgccg gtcagtgata cgccgaacga
      661 aatggccgag gtgtaccgcg cctttaatca gatggccgaa gatgtcgaac aggcggcccg
      721 cgagcgtgag ctgatgctcg ccggggtgtc gcatgacttg cgcacgcctt tgacccgcct
      781 gcgcctgtcc atggagtttc tgcacaccga ctcggagctg accgaagaca tggtgcgcga
      841 tatcgaggat atggatgcga ttctcgacca gttcctcgcc tttatccgcg atggccgcga
      901 cgagccgctg gagcagctcg acctgctcga cctgatcagt gaagtggtcg cgccctacaa
      961 ccaaaagcag gaaatggtgc gcctgtgcct ggagcctgtg ccggccttcc cgttacgtcg
     1021 ggtgtcactc aagcggttgc tggttaacct catcgaaaac gctctgcgct acggcggcaa
     1081 cgcggtggaa gtggcagttt ccctgtccgg tgattacagc gcgccttatg tggtactgag
     1141 tgtgttggac cgcggtgctg gcattgatcc ggccgagctg ggcagcatct tcaacccctt
     1201 tatccgtggc gaccgcgccc gtggtggcca gggtgctggg ttgggcttgg ccatcgtcaa
     1261 acgcatcgcc gccttgcacg gcggcagcgt tgaattacgc aaccgctccg gtggtggact
     1321 ggaagcgcgg gtttgcctgc cgttagggct gttgttaccg cgcgacgccg tctagaacgc
     1381 acaggcatac tgacatcact gcgctgaact atgctttctc agcgcgatga catgaggtca
     1441 gcatgcctgc agttccacac cgcctctctc catggacctg gttgctgccg ctgccattgc
     1501 tccatctggc cacttggctt tctctggcga cccagttcgc cgatggggca gcactctggt
     1561 acccaccgtt tgccttagcg ctggtctttg ccctgtggtg ggggccgcgt gtgctgttgg
     1621 cggtgtacct caatgccatg ttatcgatcc cgctctgggg gctggagtgg cagtgggcgc
     1681 cgctctatgc cttgccggaa acgcttggtg tagcgctggc ctgggtattg ctgcgtctgc
     1741 ggccgtttga tccggcgtta cccgacctca gtcatttgct gcgctttatg ttgcttggcg
     1801 tgctgttacc ggtcattcct gtggcgttgg gggtgcaggg taatctctgg ctgctcggcc
     1861 ctgcgtcgtt ggaagagatg cagtcgggca gcatgaccct gtggctagcc gattgcctca
     1921 ctgccctggc catcgccatt cccttgttgg cttacctgac accctggctg cgccgccgcg
     1981 gctggctagc tgcgtctgtg cgcagcggtt gcgagcaggt gcccgaggcg ctgcgccagt
     2041 tgccaccctg gccgctgctg gtgctgctgt taattggcat gccctggctg ctcggggtgt
     2101 taccgctgac cctgaatctg ccgctgattg gcgtgatgat gctcggcctg gcgttgatct
     2161 ggggttttgc cggcggcctt tgcggtgcgc tgcttagcgt gttgatggtg ctgattttgc
     2221 cgctgttgcg cggcctggtg aatagcaccg cctggcttga tccacagcgc ctggaactgc
     2281 acttcagcgt gctgctgttt atgctcgcta ccctgctggt ggggcgcagc ctgagtgacc
     2341 tgcgtcaggc gctggcacgc agtgcgcaga tgcaacagca actggcactg gccaacctgg
     2401 cactggaggc cagcccgctg ggggtgatca ttgccgatgc ccgccagcca gcgttgccgc
     2461 tgatctactg caacccggcg ttcgcgcaga ttaccggtta cagccgcgag gagaccctgg
     2521 gcaacaactg gaatttcctc ctgcgcgatg accagcagca acagggcttg agccgcttac
     2581 agcaggcggt gcgcgacggc atgggctgcg atgtggtgct gcgcaactac cgcaaggacg
     2641 gccagcagtt ctggaatgaa ttcaccctcg cgccgatgcg tgatgaccag ggcatcagcc
     2701 attttgttgc tctgcagagt gacgtgaccg cccgcgagca actggctatt caggtgcgca
     2761 cgcagagcga cgagctgctc aagcagagct atctgttcag ccagaccgag cacatagcca
     2821 acctcggcgg ctgggtgctg aacatgccgg agcaggatat ggactggagc gccggctgct
     2881 ttcgcatcta cgagttggac ccgaaaaacg gtacgcctga cctcaatggc gcattgagtt
     2941 acctggatgc cagcggccgg gtgctggccg aacaggtgct gcagcagctg attgatgggc
     3001 tggaacaatt cgatatcgag gtgcgcctgc tcaccgccaa gggtaaaacg cgttggctgc
     3061 gtatccgtgg cattaccgag cgtgacgggg ccgagctggt gcgggtgtat ggcgcgttgc
     3121 aggatgtcag cgcgcgtaaa tacgccgagc agcaactgcg tgaacgtgat gaacgcctgc
     3181 gcctgttctt cgaagcaccg ctgatcggca tggccctgac cagcccgaac tttgcctggg
     3241 aggaggtcaa ccacaagctc tgcagcattc tcgggcgctc gcgcgaacag ctgctggcgt
     3301 cgagctggga agagctgtcg ctggaggatg acctgcagaa cgagctaccg ctgctcgatc
     3361 aggtactcag cggcagccgt gaaggatttg aactggaaaa acgcttccgc cgcgccgacg
     3421 acagcctggt gcatacccgc gtcagtctgc gcgcggtgcg ccacagcacc ggtcgggtgc
     3481 gcatgtttct gttgctagtc gaagacatca gcgagcgcct gcaggccgag gcgcgctacc
     3541 gcaccatcgt cgagaatgcg cctgaagcga tcctgctgtt cgatctcaac ggaacgctgg
     3601 tcgagtgcaa tgacaatgct ctgcgcctgc tgcgttacag ccgcgaggaa attgccagca
     3661 agcgcgtatt tgatctgagc cctaacaaac aggccgatgg ccagcttacg gctgagctgg
     3721 gtaaggttta cttgaagcgg gcaatgaacg gtgaagcgcc agtattcgac tggttgcacc
     3781 gcgacagtaa agggcggcag attccttgcg aggtccgcct ggtgcgcatg cccggcgagc
     3841 cggtgctgat tcgcggcagc gtcaccgata tttccgaacg tcagcgttat cagcgtgaaa
     3901 tcgagcggct ggccttcagc gacgaactta ccggtctgcc caatcggcgt ttgctgctcg
     3961 accgtctgca gcacgcgatg gaccgcgagc tgcgcgagaa taccttgggc gcgctgttgt
     4021 ttatcgatct cgatcacttt aaaacggtca acgacagcct tggtcatctg gtcggcgacg
     4081 gtctgctgcg cgaagtcact acgcgcctgg ccggtggctt gcgcgccgaa gacaccctgg
     4141 cgcggatggg cggcgacgag tttgtggtgc tgctcgaagg gctcgacagc aacccgcaat
     4201 tggcggcgga agcggccgca gttactgcgg aaaagttgct gaaaagcctg gtcggcacct
     4261 gctggatcga cggtcatgag ctgtcgatca gcgccagcat cggcattgca ctgcatccgt
     4321 tcggcagcca agttgctgcg gatgtgctga agcaggcgga tacggccatg taccgagcca
     4381 aacagggcgg gcgcaatgcc ttgcacttct tcgccccgga aatgcaggcg gccatcgacc
     4441 agcggctgca gatgcagagc gagctgcgtc aggcggtgca acgcaatcag ctgcatctgg
     4501 tattccagcc gcaactggcc ttggtcaccg agcgcattgc cggcgcggaa gtgctactgc
     4561 gctgggtgca tccagaacgc ggcgaagtga tgcccggcga ctttattccg ctggccgaag
     4621 aaaccgggtt gattgaggag ctgggcgcct gggtgctgga gcaggcgtgc gccgcgctgg
     4681 cgcgctggtt actgcagtgg ccgcaactgg tgctagcggt caacctcagc ccgcgcgagc
     4741 tgcgccagcg cagttgtgtg gcacgcatca gcgagtgcct gcagcgccat caggtaccgg
     4801 ctacagcgct ggaactggaa atcaccgaag gtgtgctgct ggaggaggtc gagcagtgca
     4861 tcagcaatat gcaggcgctc aaggcgctgg gcgtgcgttt cgccatcgac gatttcggta
     4921 caggctattc ctcgttgact tacctaaagc gcctgccgct ggatcgcttg aagatcgatc
     4981 gcagctttac ctgggatatg gacggtgaag acggcagcgg cctgatgctg gtgcagacca
     5041 tcctggtgat cgcccgcaac ctgggcttgg aatgcgtggc cgaaggcatc gaaagccagt
     5101 ggcagcttga ctgcctacgt cagcatggtt gctcgttggg gcagggttat cactttagcc
     5161 ggccgctgaa agaggcggat ttccaggcct ggatgcagca acacagagat ccgccgccac
     5221 tgttgtgagg ctcacccctt gcccttggtg cgggtcagat ttggtccgct gtttttctcc
     5281 agatgctgaa tcaccaggct ggccacatcc ttgccggtgg tggtttcaat gccttcgagg
     5341 cccggtgatg agttcacctc catcaccagc ggtccatgat tggagcgcag gatatccacg
     5401 ccggccacgc tcaaccccat gacttttgcc gcgcggatcg cggtcatgcg ttcttccggg
     5461 gtgatcttta tcaggctggc actgccgcca cggtgcaggt tggagcggaa ttcgccgggt
     5521 ttggcctggc gcttcatcgc ggcgatcacc ttatcgccga ccacgaagca gcggatatcc
     5581 gcgccgccag cttccttgat gtactcctgc accatgatgt cctgtttgag gcccataaag
     5641 gcctcgatca ctgactcggc tgcggttgcc gtttcacaca gcaccacgcc gatgccctga
     5701 gtgccttcca gtactttgat caccagtggc gcgccattga ccatgtcgat caggtcggcg
     5761 atgtcatccg gggagtgggc aaaaccggtg accggcaggc cgataccgcg gcgtgacagc
     5821 agttgcagcg agcgcagctt gtcccgcgag cgggcgatgg ccaccgactc gttaagcggg
     5881 aacaccccca tcatctcgaa ctggcgcagc accgcgcagc cgtagaaggt caccgaggcg
     5941 ccgatccgcg ggatcaccgc atcaaagcct tccagcggct tgccgcggta gtggatctgc
     6001 ggcttgtggc tggcgatgtt catataggca cgcagggtgt cgatcaccac catctcgtgg
     6061 ccccgcaact gcccggcttc taccaggcgg cgggtggaat acaggcgcgg gttgcgcgac
     6121 agcacggcga tcttcatggg gcaccagggg agtcagcgag ggtgggtttg ttctgtacgt
     6181 aggtcagcgc cgggttgacc aacagctgcc cgtcaatcag cgccttggag ccgagcagca
     6241 cacggtagcg catggtcttg cggcaggtca gggtgaattc aaccggccat tcgcgctcgc
     6301 ccagggtcag attggtccga atcacataac gcgactgtgc gtggccattg gagcttttga
     6361 tggtcttcag agaaaccaac tgcgcttcac agcgatgccg gcgctgcacc acggtgccga
     6421 ggtgcgcgtc aaaacgcacc cagggttcgc catcgcggtc gaacggctgg atatcgctgg
     6481 cgtgcaggct ggaggtgctg gccccggtat cgatcttggc acgcaggccg atcatgccca
     6541 gctccggcag gttgatccat tcacgcaggc cgataacgcc aagttggtca aaagtcttca
     6601 aagggcgatc catggtattt gcgggcattc tagttggccc ttatgacaac tgcatcaggc
     6661 aaaatgtccg tctgcatagc cggcctaaat ggggatcaga tgagtgataa agacgacgac
     6721 aaggtacgcc tggataaatg gctgtgggcc gcgcgcttct ataaaacccg cgccctggcc
     6781 aaggcggcta tcgaaggcgg caaggtgcac catcgtggcg agcgctgcaa accgggcaag
     6841 gagcccagag tcggtgatgt ctacttgatc cgcaccggtt tcgaggagcg cagtgtggtg
     6901 gtgcaggcgc tttccgtggt gcgtcgtggc gcgcccgagg cgcaggcgtt gtatgccgaa
     6961 acggcagaaa gcatcgagcg ccgcgagcag gctgcggcgc agcgcaaggc tggagcgctg
     7021 gggatgcaga ccgatggccg accgagcaag aagcagcgtc ggcagatgca cgacttcaat
     7081 gatcggcaag acggcggcct gcgccacccc tgggctgacg aagaggtcta gcggccgctg
     7141 ataacgctca ggcgacccaa cagtggcagc ctggcgagca gacgcatcag cggaagggtg
     7201 atcttctcca gccattcgct ggctttgtag gcgaatgggg taaagcagcc ccaggccaag
     7261 cccagcaggg cgaggaatac gccgcctacc agagcgtccg aggcccagtg agcgcccgcg
     7321 accaggcgcg gcagcataaa gataacggcc atgctccaga ccagcagcaa gcgccagttg
     7381 cgcgcaaaga agctcataaa catcgcccag atcagtagca ctgaggcgtg atcgccgggg
     7441 aagctgcggg tggcgctgtc cttcatgttc cagcgctctt cccagtccgg gaacagctcg
     7501 gttaggcgtg ccgtaccctc caccaccaga gatgggctct ggcgctgcca gtccatcagc
     7561 ttgaccagct cggcaaaaac cacgcgaaac agcagcatta cgatcagcgt gacgagaaag
     7621 gcatacagag ccttgcgcac ctgcgcgccg ctgaatacca ggtcggcctt gagcatcacg
     7681 gccagcatca gcacgccaac acccatgtcc accgggcgca tgctgccgat tgcccaggtc
     7741 tgcgcccaca ggccggcgct gtgcacgggt tcgttaagca gggcaaacag ctgcaggtcg
     7801 aactggtccc acagcgcgcg gctgggcgcc cacagccagc tggcgagcag cagcagggcg
     7861 acgatatggc aggcaaccag cgggcggata cgccactgct ggttaataaa agaagggctc
     7921 atgcagtggc atccctgtca tctgtagggg attaaggcgg cgcgcatgct aggtattctg
     7981 tcttctgatt gcaaagattc gctctgcctt ttgtcggcaa ctgtgactcg ggtttgtatc
     8041 gatagcctcc gcccgcagcg ctgatagata agaggcgctt ggtcggatgg gcgtttgcgc
     8101 ctaaaatcgc gggccgagtc acattcagtg aagttggcat gtctgatttt tcccaacgct
     8161 tcttgttcga cgacagcgat attcgtggtg agctggtcgg cctgaccgac agctacagcc
     8221 atgtgctggc caaacacacc tacccgcaac cggtcgccca actgctgggt gagttgctgg
     8281 ccgccgcctc attgctgatc ggtacactga agttcgaagg cctgctgatt ctgcaggcgc
     8341 gctcctccgg tgcggtgcct ttgctgatgg tcgaatgctc cagcgaaggc gagctgcgcg
     8401 gtatcgcccg ttatcacgcg gaacagatca ctgccgatgc caacctgcgc gaattgatgc
     8461 ccgatggcgt gctggccatg accgtcgacc cgaaatccgg gcagcgttat cagggtattg
     8521 tcgatctgga aggcgacacc ctggccgatt gcttgaccaa ttacttcgcc acttccgagc
     8581 agttgcccac ccgtttctgg ctcagcgccg atggtcagcg tgctcgcggc ttgctgctgc
     8641 agcagctgcc ggccgaccgc ctgaaggaag ccgatgagcg tgaagccagc tggcagcacg
     8701 ccatggccct ggcggatacc ctgaaggccg aggaattgct gggcctggat aacgaaaccc
     8761 tgctgcaccg cctgtatcac caggaagcac tgcgtctgtt tgacccgcgc gccctgcagt
     8821 tccgctgcag ttgctcgcgt gagcgttcca gccgcgcgct gatcagcctc ggccaggccg
     8881 atgccgaggc gctggtgcag gaacacggcg gcagcgtgga aatcgattgc cagttttgta
     8941 acgagcgcta tctctttgat gccgccgatg tgctgcaact gtttattgat gggagcatcg
     9001 aagctccatc ggatacgcga cattaaggca aaatgctgta aatcgcgctg ggaatgatct
     9061 gatcagaggc tgcacccgca gccggctttt tctggcataa tccggcgcac ttttttcgct
     9121 gtagtagtgc tcagctttct actacaaaac gttcggaaga ctcggccgct tggccgacgg
     9181 ggacccacat gatgcaagcc aacaacgccg tgtacaccga tatcagtgtt gcccaactgg
     9241 tcgaagaagc ccttcgtcgc ggtgaaggcg aactggccgc caacggttcc ctcgtcgtac
     9301 gcaccggcca tcgcacgggt cgttcgccgg ctgatcgctt catcgtgcaa gagccgagca
     9361 ctgaggcgaa aatcgcctgg ggcgcaatca accgcccgtt cccggctgac aagttcgatg
     9421 ccctgtggga ccgcgtacag gcattctctg atgcgcagga cagctttgtt tcctatgttc
     9481 atgtaggttc agccgaagcc cactacttac ccgtcaaaat gaccaccgcc accgcctggc
     9541 agaacctgtt cggccgtcag ctgttcatta acccgccgca atacaacccg gcggataaag
     9601 gcgagtggca agtgctcaac gtcgccaact tcgaatgcgt gccggagcgt gatggcacca
     9661 attccgacgg ctgcgtaatc ctcaacttcg ccgccaagaa agtcctaatc gccggtatgc
     9721 gttacgccgg tgaaatgaag aaagccatgt tcagcgtgca gaacttcctg ctgccggacg
     9781 ccgacgtgct gccgatgcac tgcgcggcga acatcggtga agaaggcgac gtgaccctgt
     9841 tcttcggtct gtctggcact ggcaaaacca ccctgtcggc tgacgaaagc cgctacctga
     9901 tcggcgatga cgagcacggt tggggtgtgg gcgtggtgtt caacatcgaa ggcggttgct
     9961 atgccaagtg catcgacctg tcggagaaga acgagccggt gatctggaaa gccattcagt
    10021 tcggcgccgt gttggaaaac gtggtgctcg atgaaaaccg cgtaccgaac tacgccgacg
    10081 acagcctgac ccagaacagc cgtgcggcct acccgctgga gctggttgag aagcgcagcg
    10141 agaagaacct tggcggcgag ccgaacgcgg tgatcttcct cacctgcgac ctgactggcg
    10201 tactgccacc ggtatcgatc cttaacgaag agcaagcggc ttaccacttt ctctccggct
    10261 acaccgcgct ggttggttcc accgaaatgg gttcgggcgg cggcatcaag tcgactttct
    10321 ccacctgctt cggcgcgccg ttcttcccgc gtccggctgg cgaatacgct gaactgctga
    10381 tcaagcgtat ccgcggcttc ggctccaagg tttacctggt caacaccggc tggactggcg
    10441 gtggctacgg cgttggcaag cgcttcaaca tcccgaccac ccgtggcgtg atcgcagcga
    10501 tccagagcgg cgcgctgatc ggtaccgaaa ccgagctgct gccgatcatc aacctgagcg
    10561 tgccgaagtc ggtaccgggc gttgaaacca acctgctcaa cccgcgcaac acctgggcag
    10621 ataagaacgc ctacgacgaa gccgcaaaag gcctggccaa gctgtttatc gaaaacttcc
    10681 agaagttcga tgtgagcgac gccatcaaga atgccggccc gcagctctaa gaacctgctt
    10741 acgatctgct gcgcgtcggc cctgcggcgt tagaaacagg ctcggaatgc tcatgtacag
    10801 ctcgtacact ccgcttcctc gcctgtttct gccttgcatg gctctagctc gcgagatcgt
    10861 aaacaggctc taagctgtta ttgccgcgtt gaaaaaccgc cttaatcggc ggttttttta
    10921 ttcatgggaa attaagcatc aatctgtagg aggcgcttta gcggcgagct tttaagaagc
    10981 tctcgcggct aaagcccctc ccagggtgct ttgcatacgc gataagaaac ggaaaagccg
    11041 cccgtaggcg gctttgggtg cgtatctgct agcagatcag gcacttttgc taggggttgc
    11101 gcgttgcttt cttactcagg tggccgctct gcacggcctt agatgatgct ctatggtgtc
    11161 tcgggccaca gcactaacgg ggttggcaac tcaggcgatt tccggggcca ttgtctgcga
    11221 ctgtcgactt cggcatgact ggctagtgcc aggtaggtgt cgatatgtcg cgggtggccg
    11281 ctggcgcttt cgagttgtat ggcggcgtta ggtctatggt ttggcacaac tgacggcgaa
    11341 cctgagttct cccgactggg ttgcgcatcg gcctgttgtt cgcatcagct actccggttc
    11401 ttccggggtc tatgctgcgc ggccatctgg cctgggtggt gagcgatcta gtgggtaacc
    11461 agcaagctgc cgctgaaacc agatacctgc atactcatct ccttttttat tggcatggtt
    11521 tgaatgtacg cctgtgttgc ccatggtgtt agttggattg gcaatagcgg cgcggcttgt
    11581 aggaattaat cattgtttac cgccagggcg gtcgatggag tgacagctat gagtaccttg
    11641 gaacgtgtgt tcggctttaa gcaattgcgc cccggtcagg agcgtgtggt gacggccgtg
    11701 ctggctggcc gttcggcggc ggctatcttc cccacaggtt cgggcaagtc cttgtgctat
    11761 cagctgccgg cgctgcacct gccgcacctg actttggtgg tctcgccctt gctggcgctg
    11821 atgcaggatc agttggcgtt tctgcatgcc cgtggcatca gcgcggccag tatcgattcg
    11881 gcgcaaagcc gcgaggaggc cagtgcggtg atggcccggg cgaaatccgg cgagctgaag
    11941 attctgatga tctcggtcga acgcctgaag aatgagcgct ttcgcaactt tatcgcccag
    12001 gtgccgatct ctctgctggt gatcgacgag gcccattgca tttccgaatg ggggcataac
    12061 ttccgtccgg actatctcaa actgccggac tatcagcgcc agttcggcat accgcaggtg
    12121 ctgctgctca ccgccacggc aacgccgccg gtgattgccg atatgcagca gaagttcgcc
    12181 atcgccgccg aagatgtggt caccaccggc ttctaccgcg ccaacctcaa cctgttagtc
    12241 gagccggtta gtggtgccga caagcagcgc cgtctggaac aatggctggg agccaagacc
    12301 gggcagccga gcattgttta cgtgacccag caaaagaccg ccgagcaggt ggctgcgcgc
    12361 ctgagtcagc gcggcattgc cgccagcgcc taccacgccg gcatgccaca tgaggcgcgc
    12421 gaggcgattc agcgccagtt tatggccggg cagatcaact gcatcgtcgc caccatcgcc
    12481 tttggcatgg gtatcgacaa ggcggacatc cgcaatgtgg tgcacttcga cctaccgaaa
    12541 tccattgaga attacagcca ggaaatcggt cgcgccggcc gtgatggcca gccatcggac
    12601 tgcctggtgc tggccaatcg cgacagcctc aacgtgctgg aaaacttcgt ttacggcgat
    12661 acgccggagc tggaaggcat tcgctctgtg ctggataagc tgctgagtga gagtgataac
    12721 ggcgaatggg agctgatgct caaccagctc tccgagcaga gtaatatccg ccagctgccg
    12781 ctgaaaactc tgctggtgca gcttgagctg cgcggcatca tcgctccgcg ttatgcctat
    12841 ttcgccgaat atcgcttcaa gttcctcttg gagcccgaag cgctgctggc gaaattcgag
    12901 ggtgagcggc ggcagtttgt cgaggcgctg atcagcactt cgcaacgcgc gcgcacctgg
    12961 tgcacggtgg atttcgagca gctctaccaa cagcatcggg ccgagcgcgg ccgggtgatc
    13021 aaggccctgg attactttca ggaaaagggt tggatcgagc tggaaagcaa acagatgacc
    13081 gaggtctacg cgctgctcga cccaggcttc gacccagttg ccctgagcgg cgaattgcat
    13141 ggttatttta gccagcagga ggccagcgaa atccgtcgga ttcacgccat gctcgcgctg
    13201 tttgccagcg agcagttcct gagccagcgt ctggccgcct atttcggtga cgccaacgcg
    13261 ccgcaacagt gtgggcattg ttcggtgtgc cgtgggcaag tggcccgact gcccgcaccc
    13321 ccggctttac cgccgttggc acagcaggat gcacacgggc tatgcgacgg cttggtgcgc
    13381 aaacatcagg aactcaaagg cagcgagcca agcgccgaat gcctgacgcg ctttctctgc
    13441 ggtatcagcg tgccgctgtt taccaagctc aaggcgcggc agctcggcgg ttttgccgca
    13501 ctggaagatt atccctatgc cgaggtgcgc gagtggttga ggggggcgta gggtggacat
    13561 ggcgaagcct tgtccaccac gagggtttga gcggcggaca aaccttcggc atgtccgccc
    13621 tactcggtgt tgcgcatgac gagggaggat cgctgactac actgtatcca tgatcttgag
    13681 tacaaggatg cactcatgcc gaatgaccgt cgcgcctggg taccaggggg tacctacttt
    13741 tttaccgtta atctgcagga tcgcgagagt gatctattgg ttcgtgaaat cgccttgcta
    13801 cgccgcgtgg tagtcaccac caaggcgcgc catccgttcg ctatcaatgc ctgggtggtt
    13861 cttcccgaac atatgcattg cctgtggaca ctgccagcgg gtgacagcga tttcgccagg
    13921 cgctggatgg tgatcaagca acttttctgt cgtggcctgc ctgcagccac tggcgctaac
    13981 gcaaggcgtg agcgcggcat ctggcagcgc cgctattggg aacatctgat tagagattca
    14041 caggactggc gcgcgcattt cgattacatc catatcaatc cggtcaaaca tggctgggtt
    14101 agtcgtatac aggattggcc ctattccagt tttcataggg cggtacgtga cggtctatat
    14161 ccgcaggatt gggcggggaa tgtagatggg gtggtacctg gtggtgactg actggcgaag
    14221 gcttgggggg ttattgcgta gggtgggcat ggcggagcct tgtcgaccat ggggattgaa
    14281 acggcggaca agccttcggc atgtccgccc tacctgctag gcactactcg ccgatatcct
    14341 cgttccacag cgtcggctgg gcggcgataa agctttgcat catgtgcagg cattcgctgt
    14401 tgtgcagcac gtcgagctgc acgccgtgtt cgcgcagcag tgcttcttcg ctgagcccat
    14461 gggcctgccc ctcgctgtcg gtgggcgggt tcagttcact gcagctgcgc tgtctggccc
    14521 tgctttaggg tccattccag cacctcgcgg gccagttgat cgccggcctg accgaaggcg
    14581 ttaaccaccg ccggcaccga ggtgtcgttg gccggctggc tgacttcgaa acgtcggctg
    14641 gcgaggatgc gcaggctgcc gctttgtacc aaacgggctt cgaacaggat gcgcgcggcc
    14701 gggcggccgt cctggtattc gctgtggaag gcgcgcaggt cgccgtccag ttccagatcg
    14761 gcctgcaggc ggctttcatc gctgctcacc gccttgaggc ggccgtcgtc gaggaaggcg
    14821 tcgatcagcc ggtcacgcag caataacggc gcacggtcgc tccagcgcac gccctggtag
    14881 gcgctgatct ggtcgcccgg cggcaatacg gcaatccgcg tgctgtcgag cagttggctg
    14941 ctgtagggct tgtttacccg cagcgcccaa tcggcgctgg cgtcgtggct gggcaggctg
    15001 ctggccggca gacggtagat gcttagctcc tggcttttcg gcaataccga gcaggcggcg
    15061 aggctgccga ttagcagcag ggcggcgagc aggcgaagac ggctcatggt tgaaactcct
    15121 tgatggtgtc gctgcgcagc aggtagccgg ccgggtcttc ctccaggcgg cgggcgaagg
    15181 agcgcagggc gctgagggtt tcgcgcagtt cgataatggt cgggcccagc tcgccgattc
    15241 cctgcatacc gccgtcgaga gcggcgcggt tgtcggtcag caggcgctcg acgttgctgc
    15301 tgctacgctc cagcgaggcc atcagacggc tggcgctgtc catcgcttcg cggccctggc
    15361 cgctgagcag ctggttggcg ttgcgcatca gatcggcggc ttcctggctg gccgtgctca
    15421 tctgctgcag ggccgtgcgc agctcgtcgc gctgctcggc gacgctggcg ctggtttgtt
    15481 cgatattctc cagggtgcgc gtaatacgcc cgatattgtc gcgtgagaac agctggttgg
    15541 cgcggttgag caggcgcgta acgctgagta ccaggtcttc gccattggac atcagcctgg
    15601 agagcggtga gcggtcggcg atgatctccg cgtgttgctc gcccttgctg accagcaccg
    15661 ggctttctgg cgagccactg tgcagctgaa tcagcgccgc gccggtgatt ccggtgatcg
    15721 acaggcgggc gcgggtgtct tctttgatcg gcgtatcggc gttgacgcgg atatgcgcgc
    15781 gcaccttgcg tggatcgttg gggtcgaggc gcaggctgat cacgtcgccg acctttatcc
    15841 cgctgtattg cacggcgctg ccctgggaca gaccgctgac cgcctcgttg aagatcacgt
    15901 catagtcgcg cagttcctga tccgagctgg ctttgctcag ccacagggca aagcctaatg
    15961 cggcggcgac catcagcacg gtaaacaggc caatcaacac gtggtgcgca cgcggttcca
    16021 ttcagttgct ccctggcgcg ccggtggcgg cctgttcggc tgcgcgtccg cgcgggccgt
    16081 gaaaatagtc gtgtatccaa gggttgtcgg tggcggccac gacatccagg cggtcggcca
    16141 ccagcacgcg cttttccgcc agcacggcca cccggtcgca gatggtgtag agcgtatcca
    16201 ggtcatgggt taccagaaac acgctgaggc ccagggcgtc gcgcagggtc agaatcaatt
    16261 gatcgaaggc ggccgcgcca atcgggtcga gcccggcagt gggttcgtcg agaaacagaa
    16321 tgtccggttc cagggccagt gcgcgggcca gggctgcgcg tttgatcatg ccgccggaca
    16381 gttcagttgg gtatttgtcg ccggcttcac gcggcaggcc gaccagcgcc agtttgacct
    16441 gggccaggcg ctcggcagcc ggacgtttga ggccggcatg ttcgagcagc ggcagggcga
    16501 tgttttcggt caccgtcagc gaggaaaaca gtgcgccgcg ttgatagagc acaccgaaac
    16561 gccgctccag ctgcgagcgt tgctcgggcg gcaggcttag caattcctcg ccaaataccc
    16621 gcacggtgcc ggagttggcg cggcgcaggc cgagaatact gcgcagcagc acggacttgc
    16681 cggtgcccga gccgccgacc acgccaagca cttcgccgcg atacaggtcc agcgccaggt
    16741 cctggtgcac ggtctgcgcg ccgaagcggt tgaccaggtt gcgtacctcg ataatcactt
    16801 cgcccttcac cagcccatct ccatcagaaa cagcgcggcc aaggcatcga gcacaatcac
    16861 cacgaaaatc gactgcacca cgctggaggt ggtgcgctcg cccaccgact gcgcgctacc
    16921 ggtgaccttg aagccttcca ggcagccgat tacggcaatc aggaaggcaa atatcggcgc
    16981 cttggccatg cccacgacaa agtgcttgag ggcgatattt tcctgcagca tggccaggta
    17041 catggccggc gagatatcca ggctcagggc gcagaccacc gcgccaccga ggatgccgct
    17101 gagcatggcg ataaaggtga ggattggcag ggcaatcagc agggcgaaca cccgtggcag
    17161 caccagcagc tccagcggat tgaggccgag ggtgcggatc gcgtcgattt cctcgttggc
    17221 cttcatcgag ccgatctgcg cggtaaaggc gctggcagta cgcccggcca tcagaatggc
    17281 ggtgagcagc acgccaaatt cgcgcaagaa ggagaacgcc accaggtcga ccgtgtagat
    17341 gctcgcgcca aagtcggcga gcactgtggc gccgagaaag gccaccacgg cgccgaccag
    17401 aaaggtcagc agggcaacga tgggcacggc attcaggccg ctctgctgca aatgcgcgac
    17461 cagcgaggtg atgcgccagc ggccggggcg cagaatgatg gcgaacatcg ccgcgagggt
    17521 catgccgata aagccgagca gcgccacgcc ctgctgccag agttcttcca cggcttggcc
    17581 gatctgtccg aagaattcgc gccaggccgg gatgcgcttg cttggcagag catccccagc
    17641 atcggcgatg gcgttgccca ctgcctgcaa caacgcgcgt cgctcgctgg ccaggtccgg
    17701 cgcatcatta gccaattccg ccaggcgctg actgccgagc aattccacca acagcgcggc
    17761 gccggcggtg tccagcgcgc caagctctcg caggtcgata ttggcggcgc tgttcagtgt
    17821 gccgcgcagt gccagcactt cgacttccag gctggcgtag tgaggcagcg tccagtcgcc
    17881 gccgatgcgc aagccagagg gcgcatccgt gcgactggat tcaagcggct tgataaaggg
    17941 ggctgtgctc atggccacag cttaaccctc tagctgaggc tacgcccagc ctgcttgcgg
    18001 ttcattgcgg cttaaccgtc tgcggtaatt tcggcaatca gctcgcgcag cgctgcttcg
    18061 gcatcctcgt tggccacctg gcaggtgccg gcattggcat ggccgccgcc gttgtagctc
    18121 aggcacagct caccgacatt ggtgttggag ctgcggtcga ggattgattt gccgatggcg
    18181 aatacggtgt tctgctgctt tacgccccac attacgtgga tcgagatatt gcactgcggg
    18241 tagagggcgt agatgacgaa gcggttgacc gcataaatcg tctcctcgtt gcgcagatcg
    18301 agcaccacta ggttgtggta gaccgtggca cagcgctgga tctgcgcctt ggcttcgcgc
    18361 tggtgctcca ggtacagcgc cacgcgctca tcgacatccg gcagttcgag gatttcatcg
    18421 atgccgtggg tctggcagaa gtcgatcagc tgcatcatca gctggtaatt ggaaatgcgg
    18481 aaatcacgga aacgcccaag gccggtgcgc gcatccatga taaagctcag tagctcccag
    18541 ccatgcgggt cgagaatctc gtcgcggctg aattgcgcgg cgtcggcctt gtccacggcc
    18601 tccatcattt cgtcccactc ccgcggaaag gcctcatggc cgccgtagtg cttccacacc
    18661 acccgcgccg ccgagggcgc atcggcatcg atcaggtgat tgctcgccgg ctggtttcgg
    18721 atggtttcgc tgaggtggtg gtcgaaggcc aggtagcagt cggggacgta gggcaggttg
    18781 gtggtgatgt cgttgctggt gatcgccacc ttgccgtcct gcatgtcctt ggggtggacg
    18841 aagagaatgt catcgatcag gttgaggtgt ttgagcaggg cggcgcacac caggccgtca
    18901 aaatcactgc gggtgaccaa gcggtaaaga ctggacataa agcatctccg gatcgggcaa
    18961 gcggcgggaa taaaccacca ggccctgttc aaggtagcac gcggtttgca catcagcctg
    19021 ggcgaagggg gtgcgcttcc aggcagttgc aggtcgactt caggacagtc ccggcgcgtt
    19081 ggcggttcta ggctggcgct tgttgatcta ccgacctcag gagcccccca tgtcaccgat
    19141 taccgtactg cgtgataccc agccgattcc gctgctggat gcctgcaagt ggacgcgcat
    19201 cgccggcgag ccgcacaccg ttaacctgaa cgcctacacc tcggcggacg gcagcaaaat
    19261 catgggcacc tggatctgca cgcccggcaa atggcaggtg gcctatgaga aatgggagta
    19321 ctgccacttt caggaggggt actgcatcat caccccggag gggcaggcgc cgattcatct
    19381 gcgggccggc gatatcttta tcgtcgaacc cgggatgaag ggcacctggg aggtggtcga
    19441 gacggtgcgc aaatactttg tatttgcctg agccgtcaga ccttagggtt tgaagttacg
    19501 cgcatccttg gcccacacat ccagcgccgg cttgatgtct ttgaggctca gttgggtgct
    19561 gtcattttcc agttgcaggc cgtggccctt gcgcacaact cgcgccatag gctggctgtt
    19621 acggccgtcg atggcttcca gctcgacata aatgttggtg ttgaggtcgc gggtgccggc
    19681 ggcggtgctg gcggcagcgg caagcagggc aatcggtacc acttcataga ccttgaggcc
    19741 ttccgccgac aggtttactg cggtgattgc gctgcgtaat accagggtgt cctggtcggc
    19801 ttgttggacg acgcgatatt gcccggccag ttcgagttgc atggcctgtt gcaggtaact
    19861 ggtaatttca tccagagttt tctggctgac ttgttcacta ggggttggag cgggatagaa
    19921 caccggcttc tctactagca ccgaacggta gttctgcgct ttgaaatctg gcgacaccca
    19981 gcgcatcacc ggcgcaccgc tggccgattg cgccggctgc agctgcgagt aatcactgag
    20041 aaagcccgag tattgcgacg gctgggttgt tttgctggcg caaccggcca ataacaaggt
    20101 ggtggatagg gcaaggccaa gggttttcag tgtcattgcg gtgatctcgc tagggttggt
    20161 tgcctaactg tagaccagtt tggaataagc aatattttgc ccgtgcaagc cctctaattc
    20221 ggtatgtgtg ggtcttaatc gcggcaaaac accagggcct tcagggcgct ttccggttgg
    20281 ctgtcaggga attccggcgg gttctgcagg cgctcgctaa agcgcaggcc aggcgcttcg
    20341 ctggccatgg cctggatcag gtaatccggg ccgatttccg gctcgttgct gcaggccagc
    20401 accacaccgt tagcgttcaa aagttcgggc aagcggcgca gcaccttcgg gtaatctttg
    20461 ctcaacacga agctgccacg ctggaaggac ggcgggtcga tgatgatcag gtcatagggg
    20521 ccgtacttgg ccaccttgcc ccaggacttg aacaggtcat ggccgagaaa caccacctgg
    20581 ctcaggtcat gctggttcag tcggtgattg tcgcgacccc ggctgagcgc tgcccgtgcc
    20641 atatccagat tgaccacctg acgcgcgccg ccggccagcg ccgccaccga gaagccgcag
    20701 gtgtaggcaa acaggttgag cacgcgcttg cccgccgcct gcgcctgcac ccactggcgg
    20761 ccatagcgca tatcgagaaa cagcccgctg ttttgctttt tgcccagatc gagccggtac
    20821 tgcaggtcgt gctcgcaaat atcccagtgc tccacgggct cgccaagcag ccattttgtc
    20881 gggctctcgg gcaggtagcg ctgctgcagc aacaggctgt gtgcgccgct ctgttgccag
    20941 gcggcagatt ggccgatatc gagcagcatc tgctgcagtg cggccagttc ggcatcgctc
    21001 accggtttga acagcgccac cagcagcacg ccttgcagcc agtccacggt gacctgctcc
    21061 agccccggcc agcagcgccc gcggccgtgg aacaggcggc gggtttcatt gggcgcgcta
    21121 ttcagagcgg tgagcaggtg ttgctgcagg gtggcaaggg cgtctggggt catgggctgc
    21181 ggtcgatttg gcgagacggc gattttaaca ggcgagtgcc tagaatcgtc tgtatcgatc
    21241 attgttatcg gtaaaggcgt ttggcgttac gcccggcaag tgcctagcat tgccctcttt
    21301 cacacctgca tataaggaac tcccatgcat atcgacgaag ccatccgcag ccgccgcgcc
    21361 gtcaaaggct atgacccggc atttatcctg agtgatgccg agaaggacga gctgttacag
    21421 ctggccttgc tcgcgccgtc ggcgttcaat ctgcagcatg tgcgtatggt tgaggtcagc
    21481 gacccggcgc tgcgtcagca actgcgtgaa gtggcctggg gtcaggcgca ggtgaccgat
    21541 gcgtcgatgt tggtggtggt gtgcgccaag ctcgacagct ggcaggagga tgtcggtcgc
    21601 gtctgggacg gtgcgcctgc ggaagtgcag gcctatatgg ccggcgccat cgacacttac
    21661 taccgcgaca agccgcaggt gcagcgcgac gaaaccatgc gcagctgcgg cctgatggcg
    21721 cagaccctga tgctcgccgc acgtggcaag ggcctggatt cctgcccgat ggacggcttt
    21781 gacttcgacg ccgtggccaa gctgatcaag ctgccggcca accatgtgat cgccctgatg
    21841 gtcgcggtcg gcaagaagac catcgagccc aagccgcggg tcggcaagct gccgttcaat
    21901 gaagtggtgc tgcgcaacgg cttctgatca ttgcgcaggt ttctgagcgg tttggtcatt
    21961 cttctgccat aactgggatc agccggcggc tgttcacacc actaaaggct ggatgacaaa
    22021 agcgctgttg ggggtgaatc tggcggcgct acgcgtcggt ctgcacccaa taagaagaag
    22081 cctgaggaag ccccatgtct gccgaacatc tggatgtctt gatcattggt gccggcttgt
    22141 ccggcatcgg tgcggcctat cacctgatga agcattgccc cggcaagcgc tttgccatcc
    22201 tcgaaggtcg tgcagcgctg ggcggcacct gggatctgtt ccgctatccg ggcattcgtt
    22261 ccgattcgga catgttcacc ctcggctaca acttcaaacc ctggaccaac ccgctggcga
    22321 ttgccgacgg cccgtcgatc ctcagctata tcgaagagac tgccgaggaa aacggtattc
    22381 aggaaaagat ccgcttccag cacaaggtac tcaaggccga ctggtgcagc gagcacgcga
    22441 cctggaccct gcaggtgcag cgtggcgacg atcccgagcc gatcaaactc agctgccagc
    22501 atctgctgat gtgcaccggc tattaccgct acgaggcggg ctacacccca gatttcaagg
    22561 gccgtgagca gtttcagggt gaatttatcc atccgcaact gtggccggaa aacctcgatt
    22621 acagcgacaa gaacgtggtg gtaatcggta gcggcgcgac ggcggtgacc ctggtgccgg
    22681 cgatgaccga taaagccaag cacgtgacca tgctgcaacg ctcacccact tatgtggtga
    22741 gcctgccgca gggcgatccg atttctgcct tcctgcgccg ttttctgccg gaaacctggg
    22801 tctaccgcct ggcccgtacg cgtaacgtgg cggtgcagtt aggcttctac aaactgtcca
    22861 aggctttccc cagcctggtg cgcaaggcgt tgctcggtat ggccaaacgg cagttggggg
    22921 cgaattttga tatgcgccac tttagcccgc gctacaagcc ctgggatgag cgggtgtgtg
    22981 cagtgcccaa tggcgacctg ttcaaggtgt tgcgcaaggg caaagcctcg gtggtcaccg
    23041 agcatatcga cggctttacc gccaagggcg tccgcctgaa gtccggccag gtgctgccgg
    23101 cggatgtgat catcagcgcc accggcctgg agctgcagct gtttggcggc atgcaggtgg
    23161 cggtggacgg ggtgtccttc gatgcggcca agagcatggg ctatcgcggc atcatgctac
    23221 gtgacttgcc caatgctgcg gtggtgctgg gttacaccaa tgccagctgg acgctcaagg
    23281 ctgacctttc cagcgaatat ttctgccgtc tgattaacca tatggatgcc aatggcatgc
    23341 gccagtgcac cccgcgtgac acggcgaggc aggttaaaaa tgcgccgttc cttgatctgg
    23401 ccagcggtta tatcaaacgc gcggctgaca agatccccag tcagggtgac cgcgcaccgt
    23461 ggaagcttta ccagaactac ctgctggacc tggccttgct gcgttacggc aaggtcgagg
    23521 acggctacct ggagttcagc tcgccgcatg tcggcgagtc agccgctaca gctacagcgg
    23581 tctagactaa gctgcaatgt aagccaaacg ctatatctgc ttggcgcgtt atacctacag
    23641 aaatgtggcc atttgccgct ttataagcag gcacagggcc actatgcttt gtgacagata
    23701 tcatggatga atgagccagg tgattctggt tgttgtcaca gaggagtcct ctagaggatg
    23761 agcagaatgg aatcgcttca acaagagcaa cgctacctgc cagagctgcg agcgcacatc
    23821 gacaagctgc tggccaaagg ctgggtgatc gccgagcgca gcccgctgac cctgcagtcc
    23881 gggcgcaaga cctatatggt tgtgcacggc atgttgattg gtgataacgc tttataatct
    23941 tgccccaccg ttttcccggg ccgctgctga tcgaatgatc ggcagcggcc ttttttctgg
    24001 cctgggttag ccagtatggc gcggcggttg ccagtgggtt gccgccagcc agtcggcaaa
    24061 ggccgccggc ggtagcggtt tgcttagcag gtagccttgg gcaatgtcgc aaccaagttg
    24121 gcccaggcgt tcaagaatgg cctcggtttc taccccttcg gcaacgatct tcaggcccat
    24181 gttgtgcccg agctcgatgg tcgagcggac gatgatgtcg tcgtcgggat tgctcaacag
    24241 gtcgaggacg aaggacttgt cgatcttcaa ctcatgcacc ggcaggcgtt ttagctgggc
    24301 catgctcgaa tagccggtgc cgtagtcgtc gatcgacagt tgcacaccca attcgcacaa
    24361 ttcctgcaat tgacccatgg ccagctccgg gtcggccatc accgcgcttt cggtgatctc
    24421 aacaatcagg ctggatggcg gcacgccatg ctcggccagc tgggcggcga taaaaccggc
    24481 aaagcccgga tcggccagat ccagtgcgga aatattgatc gcggtcttca gctccagccc
    24541 ctgttcctgc caggcacggc tctgtgccac ggcggtgcgc accacccagc gggtcagggc
    24601 gcagacgttg ccggtctgct cggctagggg aataaattcg tccggcggaa taaagccgtg
    24661 taccgggtga atccagcgca ccaggcattc cacgccatac aggctgcgtg cgcggatatt
    24721 cagcttgggc tggaagtaca ggctgagttg gccatcttcc accgcacctt tcagctcgct
    24781 catcagggcc aggcgcagca ggctgtggcg atccagttcc gggcggtaga gggcgtaggg
    24841 cgaacgctgc gccttgccca ggtacatcgc cacctcggca tgctgcagca ggctgccggc
    24901 actttcgcca tgatccggat acagggcgat gcccagggtg gcgttgagtg tcatgctgat
    24961 accgcgcacg gcaaagggct cgcgcagggc ggcgcggtaa tgctcggcgg cggggatcac
    25021 ctggcccggc tggcagcggc tgatcagcag ggcaaactca tcaccaccga gacgagccag
    25081 gtgttcgcct ggtcgcagca gcgcctgcag gcgagcggcg agctggcaaa gcagctggtc
    25141 gccagcatcg tggccaaggg tgtcgttaag gtccttgaag ttgtccagat ccatcagcag
    25201 caccgccacg ccttcgcccg gcgaactgtg cgccagagcc tgttgcagtt cggcgacaaa
    25261 gtggttgcgg ttggccagct cggtcaacgg gtcgcggtag gcgagaaagc tgatggtggt
    25321 ttcgcgctcg gcaatgcctt gctgcatggc gacgaattcg cgggcgagca agccgatttc
    25381 accgctggcc tgcgtcgccg gcacttgcat cgccacctga tagtcgccgc tggcgatgcg
    25441 ccgtacgttg tcgaccatct ggcttagcgg gcggctgacc gtattggcga tccacagcgc
    25501 gccgagacca gcgagcagca atgcgatggc gaagatggtt gccagttgcc attgcagcgg
    25561 ttggtaatcg gccagctcgg cggacagcga gcgcatcagc agtacctgca attcgctgct
    25621 tgtgccgatt aatggcgcac gcagggcgac ctgttccaac ccctgtaatt caaagcgaaa
    25681 taccccttcg gcattcgtca gtaactggcc ggccagttca gcccgtggca gcgcttgcaa
    25741 gttgctggcg agtacccggt attggccagg actgccgtcg atcagggcga catcggcgcc
    25801 gctgagtcgg gccaggtgcg cggtcgcctg atcgtccagt tcaaaagcca tcagcagcca
    25861 gcccatcagg ttggggcgcg gggcgaagat tggcgtggcg ttgatatgca gcactcgctc
    25921 gccaaacacc accagacgtt cggcgctctg cgggtcttct tcatccagca ggtcatccca
    25981 tggcagcgcg gctccgggca gcagttcacc gctggtgccc gcgagaatca gcccctcggg
    26041 cgcactgacc agggcaaagc tggcctggct gcgtgaggcg aaggattcca gtgcggctga
    26101 cagtgactgc gcttcctgct gcgggctggc gatcaggtcg gcgatttcgc cgagcagggt
    26161 gaaatccttg gcgatcagat cagccatggc gctttgcgcc tgacggcggc tctgcagctc
    26221 gttgctcaac accgcatggg cgaagttcag ttgcccggcc acctggctca gggtgtgctg
    26281 atacgtggag cgatagacca gcgccagtag tagcaccatg accgccagca acagcagcag
    26341 aaagaagccg agaatcttgc cgcgcagggt catggcgcgt agccgccttt cccctgtgag
    26401 cgcatggcgg gtgtttttgg ccgtgggtcg cgtttcagtg tgccggtcag ttgccaggtc
    26461 agcgtgccgt cgcttggtac ggcgcggttc agggcttcct gcgggtcggc gatgcgtggg
    26521 tgccagatat gtagggtttg tccggcggtg gcatcgaagt ccaggcgggt tttgccctgg
    26581 ctgtctgtct gggcgaacca gggagtgtcg agcaccagga taaagccgag catccagtca
    26641 tggatattgc agcccagggt gaccaggcca ggctggtcga aacgcatctc ctgcggtgcg
    26701 gccttctgct ggtgtaggcg cagctcgaaa cgcttggccg gcgagaagga gtagacgtga
    26761 tggttgatcg ggtcggagtt ggggaagttc accgtggtgc cgacctgcac cggcaggata
    26821 tagggggtga actggcgctt ttgttggtcc atcagcgccg tagcgggctg gttttgcgcc
    26881 ggccccggct cgacccagaa caccgcgtca ctgagcggtt tgccctggtt gtcgagtagc
    26941 acgatctcca gagtttgggc ctgcgccaat gtgcccaaca gcgctagcaa cagtgccaaa
    27001 gccgatttct gcatgatcga ttccccaaga ctgcacgaca gtagccgtca gtataggaag
    27061 gctttgtgcg gttgccgggt gatcagcgct gggcgatctg ctggatctgt tcgatcagcc
    27121 agtgcagcgc cgggtcgcgc tgacgctggg ccaggctgac gatttccagg tgaaacgggc
    27181 cgagggcgaa cggcagctcg aacagttgca gcggcagcag gctggcaaag tggcgcgcca
    27241 actgggtcgg cagcacggcg gccagttcgc tgctggagac gatatgtgcg gcctgcaggt
    27301 agttgggcgt ggtgtagaga atctcccggc acaggccctg ttcgcccagc cattggtcaa
    27361 ccatgccgcg agtctgacca ccgtgtaccc agaggtggcg cagctgcaga aaggtcggta
    27421 gatccatggg cccactgacc aggggatggt cgcggcgcag cgccagttgc agggtctcac
    27481 tcatccagtg ttggcgggcg aaacgcgcgg ggatgtcctc gaagcggccc agcaccagat
    27541 caagctcacc cttgtccagc gcctcggccg gcaggctggg gctcaggtgg cggatgtcga
    27601 tgcgcatgcc gggcgcctgc tggctgagct gcttgagcag gcgcggcatg cagatcagct
    27661 cgacatagtc ggtgacggcg atgctgaagt gctgacggct gctggccggg tcaaacactt
    27721 caccggcgct gaggctctgc tcgatctgct gcagggcggc gcgaatcggg gtttccagcg
    27781 cctgggcgcg cggtgtcggc tgcatgctgc gacccacccg taccaacagc ggatcgtcga
    27841 gcagttcgcg caggcgattc agggcgttac tcaccgccgg ctgactgagc gacagacgtt
    27901 cggcggcgcg cgagacattc tgctcgcgca tcagtgcatc gaacacgcgc agcaggttga
    27961 ggtcgaaggt tgaaatattc attggctgaa tacgacttat cacaagacta aatttcaaaa
    28021 atagtaacgc cttgcttatg gtggtgggcg aatttcttta ggcaactggc agaggggtgg
    28081 gcatgagcaa ggcatttgag gttcagcagg ccgccgtaat cggcgcgggc accatgggcc
    28141 gcggtatcgt gatgagcctg gccaacgccg gcattcaggt gctgtggctg gacaacaacc
    28201 cgcagatgct cgagcaggcc atgaccgtgg tggccgagac caatgcccac aacctcaagc
    28261 tgggtcgtat cagcgaagag cagtctgccg cgcgcctggc ctgcgtcacc ccggtgggcg
    28321 aatacgccgc gctggccgac gccgatctgg tgatcgaggc ggtgtacgag aaccttgaac
    28381 tcaagcagca gatctttcgt acgctcgacg ccactctgaa gccaggcgcc attctcgcca
    28441 gtaacacctc ggcgctggat atcgacgcga ttgccgctgt cactgcgcgc ccgcaggacg
    28501 tgctcggtct gcacttcttc agcccggcgc acatcatgaa attgctggaa atcgtccgtg
    28561 gcgccaaaac tgcaccagcg gtgctggacg cggctgtggc cctgggccag cgcataggca
    28621 aggtcagtgt ggtggcgggt aattgcgacg gttttatcgg caaccgcatg ctgcacacct
    28681 atgtacgcga ggcacgcatg ctgctgctcg aaggcgccta tccgcatcag gtggatgccg
    28741 cgctgcaggg ctttggcttc gctatgggcc cgttccgcat gtacgacgtg gtcggtatcg
    28801 acctggaatg gcgcgcccgc gagctggccg gcaagggcca ggacgatccc gctgtgcagg
    28861 tggataatcg cctgtgcggc ctcggtcgtt tcggccagaa ggtgcgcatg ggctattacc
    28921 gctatgccga aggcagccgc caggccgagc atgatcccga agtggatgcc ctggtgctgc
    28981 aggtatccga acagctgggt tttgtccgcc gcgagatcgg tcccgaggaa atcctcgagc
    29041 gctgcctact ggcactggtc aacgagggcg cgaaaatcct cgaagagaat atcgccgcca
    29101 atagccacga catcgatctg gtctacctca acggctatgg cttcccggcc gacaagggcg
    29161 ggccgatgag ctgggccgat agccagggtg tggcggcgat tcatgaccgt ttgaaagcgt
    29221 tgcaggcagc gttcggtgag cactggctcc cggcacgcct aattgagcag ctggcggcca
    29281 gcggtcagcg ctttgccgac gtgcgggtat ccgacatgcg ggtatccgat gtacaggagg
    29341 gtcgggtatg agttatcagg ccccgctgcg cgatatgcgc tttgtgctgc acgagctgtt
    29401 cgatatcagc cgccactgcg agctgctcgg caacggcctt gaccgcgagc tgatcgacgg
    29461 cgtgctggag gagggcgcac gttacgcggc cgaggtggtg gcgccgctca atcgccacag
    29521 tgacgagcag ggttgtcagc tgaatgacgg ccaggtgcgc accccggatg gctttgccga
    29581 ggcctatcag caatacgttg ataacggctg ggcgagcatg accgggccca gcgagtacgg
    29641 tggccaggcg ctgccgcaga tgctcgcctg caactttcac gagatgctga tgggcgcttc
    29701 cctgtcattc cgggtgtatt ccgggctgac cgaaggcgcg gtgctggcgc tgtacaaaca
    29761 tggcagcgag tcgctgaaaa acacctacct gcacaagctg gtcagtggcc agtgggccgg
    29821 caccatgtgc ctgaccgagc cgcaggccgg caccgacttg gccctgctgc gcaccaaggc
    29881 tgagccgcag gcggacggct gcttcaaggt cagcggcagc aagatcttta tcagcggcgg
    29941 cgagcaggac ctgtcggaaa acatcatcca tctggtgctc gcccgtctgc cggacgcgcc
    30001 agccggggtg aagggtatca gcttgctgtt ggtgccgaaa tttatcgccg ctgccgacgg
    30061 cacgccgggg gagcgcaacg cgctgtcctg cggggccatc gagcacaaga tgggtatcaa
    30121 gggtgcgtcc acctgcgtga tgaatttcga tggcgccacc ggctggctga tcggcgaagc
    30181 caaccagggt ctggcctgca tgttcaccat gatgaacgac gcgcgctttc aggtcggttt
    30241 gcaggggctg ggcattggcg aagcggcctt ccagggtgcg ctgcgttatg cccgcgagcg
    30301 cctgcagtcg cgcgccctga gcgggccggc ggagccgggc aaagcggctg atccgatcat
    30361 tgttcacccc gatgtgcggc gcatgttgct gacgcagaag agcctggtcg aaggcagtcg
    30421 catgctggcg gcttactgcg cacgtcagct cgaccttgag catggtaatc cggacgccgc
    30481 cgcgcgcaag gccgccggca agcgcgcggc gctgctgatc ccgatcgtca aagccttctt
    30541 caccgatatg ggccaggaag tcgccagcca tgcggtgcag gtgtatggcg gccacggttt
    30601 tatccgcgag tggggcattg agcagctgat gcgcgacagc cgcattaccc agctgtatga
    30661 gggcaccaac ggcatccagg cgctggacta tatccgccgt aaggtgctcg gcgacggcgg
    30721 tgccgaactg tcggcgttgc aggccgagtt cagcgagctg tgcgacgccc aggttaggcg
    30781 cccggcgctg gccgcgctgg ctcaaacggt gcaagcgcgt ttgggcgagt ggcgcgagct
    30841 gagcagcgaa gtgatcgccg ccagccagcg cgatgtgcag gagatcggcg cggtgtcggt
    30901 ggattttctc cagtattcgg cctatgtgtt gctggccggc ttctggctgc aggcggcggc
    30961 gcgggcgcag gatgccctgg aggcgggcag cggcgaggcg gcgttctacc aggccaagct
    31021 gcagtcggcc gacttctacc tgcgccgggt gttgccgcgg gccagcgccc atcgcgagtc
    31081 cctgctgggc ggtgcagact gtcttatggc gatggatgag gcgagtttcg ccttctgact
    31141 gaagcgcgta gcgtgggtgc aatccacgct gctgcgcgag ccatttatta caacaagagg
    31201 atcaccccat gctgcccttt agctttgcca ccaccgcgca gattctttgc gagatcggcg
    31261 cctcccagcg tctggccgag ctgtgccgcg agcgcggcgc gcagcgggtg ctgatcgtca
    31321 ccgatccggg tatcagcaag ctcggcctgc tggcggatgt actgccaggc tttgcccgca
    31381 ccgagctgac tgttgaggta ttcgatcagg tggtcgccga cccgcccgag gcgattctgc
    31441 tggccgcagt cgagcaggcc cgtgcgctta aggcgcagtt gatcgtcggt tttggcggcg
    31501 gcagttcgat ggacgtggcc aagctggtgg cgttgctcgc gcatcccgag tgcggccagg
    31561 agctggcgca gatttacggc gtcggcaatg cccgaggcca gcgcctgccg ctgattctgg
    31621 taccgaccac cgccggcacc ggctcggaag tcacgcagat ttccattatc accaccggcg
    31681 aaaccaccaa aatgggcgtg gtttcgccgc tgctgctgcc ggacttggcg gtgctggatg
    31741 ccgagctaac ccttggcctg ccggcggcgg tcactgcggc taccggtatt gatgccatgg
    31801 tgcatgccat cgaggcctac accagcgcgt tgaagaaaaa cccgctgtcc gacctgctgg
    31861 cccgtgaagc gctgcgcctg ctggcggcca atctggataa ggtggtgcat gacggcgcta
    31921 accgtgaggc gcgtcaggcc atgttgctcg gctcctgcct ggccggtcag gcgtttgcca
    31981 acgcgccggt ggcggcggtg catgcgctgg cttatccact gggcgggcat ttccatattc
    32041 ctcatggcct gtcgaatgcc ctggtgctgc cacatgtact gggcttcaac gccaattcgg
    32101 cggcgcctct gtacgccgag ttagcgccgc tggtgctggg tgagcagctg cgcgctggca
    32161 gtgagttgca gcagaccgag cagtttatcg gtgctatggc cgatttcagc gtgcgcagcg
    32221 gcctgccgac gcgcctgcgt gatgccggtg tgccgcaaga gatgctgccg accctggcgc
    32281 gtgatgccat gcagcaacag cgcctgctgg taaataaccc gcgggaaatg accgaggccc
    32341 acgccctggc catctatcag gtggcttatt aagccgcctc gctaacttca aggacccccg
    32401 atgagccaac cccaacatct gcgtagtgat taccggcatt tccagccgat caccacgcgc
    32461 tggcatgaca acgatgtcta cggtcacgtc aacaacgtca cctactacag cttcttcgac
    32521 agtgcggtga acagctacct catcgaagtt ggcggcctgg atatccataa cggcgaggtg
    32581 gtgggttttg tggtcagctc cagttgcgac tacttcgcct ccattgcctt tcccgagcgc
    32641 atcgaaattg gcctacgggt cggcaagctg ggcaacagtt cggtgcagta cgagctggcg
    32701 gtgttcaagg cggatgaaga cgaggcctgt gcggccgggc gttttgtgca tgtattcgtc
    32761 gaccgcagca gtaaccgccc ggtcagcatt cccgagaatc tgcgggcggc gttgacggtg
    32821 ctgctgatgg agccggcgct ttaggcggcg cctaggccct cccacataag taaaaagccg
    32881 ctgttaggcg gctttttact gtcagcattc tgcacttagt gatgtttgtg ctttttatgc
    32941 ttcttgtgtt ttttgtgttt cttgccgtgg tagtggtcgc gatcacgctc gctgccgccg
    33001 gcatattcgt tggccagcgc gccaccggcg gcgccaccca ggccggcacc aatggtcgag
    33061 ccggtgctgc cgccgacctt gttacccagt accgaaccac ccgctgcacc caggccgccg
    33121 cccagtgccg cttcggtctt gttgccgtcg cgtgcggtaa tcaccccgcc cgccgcgcca
    33181 cccaggccgg cgccaatcgc cgcgcccgtg ctgccaccca cctgttggcc gatcacgttg
    33241 cccagggcac cacccacacc gccaccaatg gcaaccttgc cggtatcgcc cgccagggcc
    33301 aggggtgaaa ccagggcgct gaacactagc gcagaaaata atgtacgcat acggcagacc
    33361 ttctcgatta agtgggcagg tggacttaag cctttggttt taaaaacaaa gtgtccacgc
    33421 atgctgttag agggtggttt gccgaaaaat tccctaccac tggtcgccgc tgtgatcgcc
    33481 gcgacacaac ctgcgattaa aggatgcgcg cttcagcttg atgcgcctgc aagcgaatgg
    33541 ctacgtactt tgaggtcggg gtaaagctgc ccgtgccaaa actctgtagc ggcaccagag
    33601 gattggcttc cgggtagtag gccgcggcct ggccggcggg aatgtcgaaa gccagcaggg
    33661 taaaacccac aaccttgcgc tcgatgccgt catcccagat ggactggata tcgacctgtt
    33721 gtcccggttg aaagcccagg cgcaggatgt ctgcctcgtt aacgaacagc acctggcgct
    33781 ggcctttgac cccacggtag cggtcatcca ggccatagat ggtggtgttg tactgatcgt
    33841 gggagcgcag cgtctgcagg atcaggtccg gtgtttgccc ggtgctgcgc acctgggttg
    33901 gcagcagatc ggcgggtagc gggttggcct tgaagttggc cttgtggctg gcggtcagcc
    33961 agcgtcgttc ggcagcggcg ttacccaggt agaagccgcc ggggtgttgc aggcgctggt
    34021 tgaagtcgtt gaagccagaa atcgtctcgg caatcagttc gcggatgcgc cggtaatcgg
    34081 caatcagcgc atcccagtcc accggatgat tacccagggt ggccttggcg ataccggcaa
    34141 tgatcgccgg ctccgaacgc atttgctgcg acagcggttc gagctggccg taggaggcgt
    34201 ggatcatgct gaacgagtcc tccacggtca ccgcctgcgg gccgccggcc tggtggtcga
    34261 tatcggtgcg gccgaggcac ggcaggatta gcgcatccgt gccggtggtc aggtggctgc
    34321 ggttgagctt ggtgctgatc tgcacggtca gcgcgcaacg acttagcgcg gcgtggctgc
    34381 gcgggctgtc cggggtggct tgggcgaagt tgccgcccag gccgataaac accttggcct
    34441 gaccgtcgag catggcgttg atcgcctcga cggtgttgtg gccctgcacg cgcggcacct
    34501 ggaagttaaa gcgctgctcc agcgcatcga gaaaggctgc cgatgggcgc tcattgatgc
    34561 ccatggtgcg gtcgccctgc acgttgctgt ggccgcgcac cgggcacagg ccggcaccgg
    34621 gccggccgag gttgccgcgc agtagctgca ggttgacgat ttcctgaatg gtcgccaccg
    34681 agttgtggtg ctgggttatg cccatggccc agcagatgat cactcgctcg gcgcggcggt
    34741 agagcagggc ggcatgttgc agctcgtcca ggctcaggcc ggattgctcg accagcgact
    34801 cccagctgct ggcatccagc agcgctaaat agtcatccac gccgtcggtg tgctcagcga
    34861 taaactccag gtcgaacacc gccggttcgc cctgagccag cgcttcgcgg tgccactgca
    34921 gaaggaactt ggccatgccg cgcagggcgg ctaaatcgcc acccaaggct ggtcggaaga
    34981 atgcggtgtt caacggctcg gagccgttgc taagcatttc cagggcatgt tgcggatgct
    35041 ggaagcgctc cagaccgcgc tccttcagcg ggttgaagca caccacctgg gcgccgcgct
    35101 tcaccgcttc acgtagaggt tcaagcatgc gcgggtggtt ggtaccaggg ttctgcccga
    35161 gcacgaagat cgcgtcgctg tgctcgaagt cggcgaaggt cacgctgccc ttgccgacgc
    35221 ctacgctctg cccgagggcg acgccgctgg cttcgtggca catgttcgag cagtcgggaa
    35281 agttgttgct gccaaaggcg cggacgaaca gctgatagag gtaggccgct tcattgctgg
    35341 cgcggccgga ggtgtagaac tcggcctgat tggggctttc cagcgcctgc agatgttcgg
    35401 cgatcagggc gaaggcggcg tcccagctga tcggccggta gtgatcgctg gcggcgtcat
    35461 agcgcattgg ctcggtcagg cggccctggt attcgagcca gtaatcgctc tgctcgcgca
    35521 gctggctgac gctgtagcgg gcgaagaagg ccgcatccac gcggcgcttg gtggcttccc
    35581 agttgaccgc cttggcgccg ttctcgcaga acttgatgcg accgtcttcc ggcgaatccc
    35641 cccaggcgca gccggggcag tcgaagccac cgtgctggtt ggtcttgagc agggcacgga
    35701 ggttcttgaa cggctgctta ctgtccagcc agaagcgcgt cacgctgcgc agcgcgcccc
    35761 aaccggcggc cggcccgctg tagggttggt agcgcggatt gatcggttgc aggctcatgg
    35821 ctgggcgact ccgggcgctg ccgggctgta cacacgtggc gcgctttgct ggggcaggtg
    35881 gatcaggttg aggttgtgct ggcgcgccca ctgcacgctg agggtggtcg gcgcggacag
    35941 gctgaccagg gtgccgatac cggcgcgcag ggccttgtgg atcagctcca ggctgcaacg
    36001 gctggtgacc acggcaaagc cctggcgcag gttttcccct tcgcgatgca gggcgccgag
    36061 cagtttgtcg agggcgttat gtcggccgat atcttcccgg cataggcgga tctgcccctg
    36121 gccatcgata aacagcgcgg catgcaccgc gccgctgtgg cgagccatgg tttgtacggt
    36181 ttgaatccgc tgacgcaggc cttgcaggtg gacggctgcc ggcaggggac tgggcgtgag
    36241 tctttgcagc tgcggcagcg cttgctccag cgcttcgacc ccgcacaggc cgcagccgct
    36301 gttgccggct agcgtgcggc gctgctgctt gagtgcccag aaggcgcggt tgctgacctg
    36361 cacctcggcg ctgatggcgc tgtcatgctg ctgtaggcgg atatcgtaga tctcatcgaa
    36421 gctgtcgatt aggccggcac tcaggctgaa accgtagata aaatcttcaa gatcactggg
    36481 cgaaaccatc atcacgctat ggctgatgcc gttgtaactg atcgccagtg ccgtttctgt
    36541 ggccagcgcc gccgcgccat ccagctgcgc ttctgccagt tcgacatagc tataagttgg
    36601 cgccggcagc agcactgggg cggcatcttt gacgattgca ggggcgtggc gtggcatggg
    36661 caggcttccc ggcgacgaag tgacttcgcc agcctaggcc gctctgccga gggcgtctaa
    36721 tcgctgttac tgatcttgtg atcgacgctg tctatcaagg ctttaagtct ggcctttcag
    36781 ctcgcagcag cgggtagcag cttgtgtgct tcggcaaagc aggcttcggt cagggccgag
    36841 cgcggcgcac tgcggcgcat gatcaggccc agcggggcga gggtatgggc gtcttcgatg
    36901 ggcgtcagcg ccaggtgctt ggtgaattcg gctaggccgc tgttgagcgg catgatcgcg
    36961 cagcacagcc cggcgctgac cgcttgcagc agttgatgca cggcatcggt ttccaaccgt
    37021 gggctgggcg tcaggccacg ggagcgaaag ctgtggtcga tcgactggcg aaaatgcatg
    37081 ccggccgaga gcaggcccag cggcaggtcg atcaggcttt cccagcgcag cgccgggcgg
    37141 gtgaactgaa aatgccggcg gtcatacagc aggcccatgc gcgtttccgc cagctccagg
    37201 ctgtcgaaat gctcagggtt gaggcggtcg aggtaggaca ggccaaggtc gagctgatta
    37261 cgccccaggc gttcgagaat ctcttcgctg ctcagggcaa acaactgaaa gcgcagcccg
    37321 gcatgctgct cggcgaaaaa ctgcaccagg cgcatcggat cgaaaccggc cagcggcacc
    37381 acacccagac gcagggtgcc gaccagctgg ccacggcagg cggccgcttc tgcatacagg
    37441 ccatcgtgcg cggccatcag gctgcgcgcc caggccagaa tacgctcgcc ttcggcactg
    37501 aaaccctcga aacgctgacc acggcggacc aactcaagac ccagctcgtc ttccagctga
    37561 cgcaggcgca tcgacagggt cggctgggtg atatggcagc gcgcggcggc ttggccgaag
    37621 tgacgggttt catccagggc gatcaggaat ttcagttgtt tgatatccac gatgaatcct
    37681 taaaatccct gggttgcgcc gattctagca ggcagccgcg tgcgcctagg cgctaaatac
    37741 ggcctatggc gcggcctgca gcgacagtgg tgggcgtggc acctttgtca cgaatccggg
    37801 tctagacttc tggctccggg cggcgtcgcc cggccacttt ccaaggaatc aagaggaagc
    37861 cttatgagtc tgtttgcctt tgtcaaagaa gccgggacca agctctggga gtcgttggtc
    37921 ggtcaggagg cccaggcggc cgaatcactg acggcgcacg tcgccaaagt gggcttgggt
    37981 aacccgaata ttcagatcag cgtcgagggc gataaagtca ccgctcgcgg tgaggtggcg
    38041 agccaggagg agaaggagaa aatccttctg gccctcggta acgtcgccgg tgtggcgtca
    38101 gtggaggacc atatcagcgt gaccacgcca gccccggcgg cgcgctttgt cacggtgaag
    38161 aagggcgaca ccctcagcgc gattgccaag gccgaatacg gcaacgccaa tgcctaccag
    38221 aaaatcttcg aagccaacaa accgatgctc agccacccgg acaagatcta tccgggccag
    38281 gtgctgcgta ttcccgagga ataaacggct tgcgtgtcgg gcggctgcaa tggccgcccg
    38341 ctacgtttat agcccttgca acaactcccg ataatcctct acggcagcaa actcctcggt
    38401 gtccttcggt gccgcctggc tatcgggctg gcgcacggcg agcaggtggc cgacgccata
    38461 acggccggca ctgcgcagga tcggcaggct gtcgtcgata aacagactgc gtgcaggttc
    38521 gaagctgatg tcttgctgca gggcatgcca gaactgctgg tcttccttgg ggaagccata
    38581 gtcgtgggaa ctgatcagcc ggtcgaagta gggcgccagc tcgatgcgtt ccagcttcag
    38641 tgacagcgaa tcgcggtgcg cattggttat cagcaccacc tgcttgccgg cttggcgcag
    38701 ggcctggata aaggtgtcgg cgtccgggcg cagggcgatc agatgcgcca cttcgcgttt
    38761 gaggtcgcgg atcgacagct tcagctcgcg gctccagaaa tccgtgcagt accagttgag
    38821 ggtaccggcg tgttcgcgaa acagcggcag cagctcggcc tcggccagcg cgcggctgac
    38881 gccgtggtgc tcggcatagc gctgcggcag gtgctgcaac cagaagtggt tgtcgaagtg
    38941 caggtcgagc agagtgccgt ccatatccag caggacggtg tcgatggcgt tccagggcaa
    39001 cgagggcatg cgcagttctt cgggctgatg cgtagggagt cgcgatttgc gccatcggcg
    39061 tcgtgatcgc gcaatctcct acgcagataa taggaaaagc cgcgttatga taacccgcta
    39121 tgtcctgccc aggagccgcc ccatgcgtca gaaacccacc gtactcgctc gcgaaatcgt
    39181 cgccagcagc cgcctgttcc gcgtcgagga gctgcaactg cgcttcagca acggcgtgga
    39241 acgcacctat gagcgactgg tgggcaaggg cgcaggttat ggcgcggtga tggtggtggc
    39301 gatgatcgat gacgagcacg cgctgctggt cgaggaatat tgcggcggca ccgatgacta
    39361 ccagctgtcg ctacccaagg gcctgatcga accgggcgag gacgtgctgg aagcggctaa
    39421 ccgcgagcta aaggaagaag ccggtttcgg cgcgcagcac ctggaactgc tggccgagct
    39481 aagcctgtca ccgggttata tgagtcagaa gattcaggtg gtgctggctt cgggtttgta
    39541 tgaagaacgc ctgccgggcg atgagccgga gccgatgcgt gttgaccaga ccagcctgcg
    39601 tgagctgtcg agcctgatcc agcatccgca atttaccgaa ggtcgcgccc tggccgcgct
    39661 gtacctggcc cgcgatgtgc tgactcagcg tgggattttt aaaccatgag ccatccgctg
    39721 attcctgcag tcatcgaact ggtacgccag gccggcgccg cgaccctgcc tttctggcgc
    39781 gccgacgtga cggtcaatga aaaagccgac gcctcgccgg tgaccgccgc cgatatggcc
    39841 gcccaccata ttcttaacga tggcctgttg gcccttgctc ccgatatccc ggtgctgtct
    39901 gaagaggcgg cggatattgc cctgagcgag cgcgggcagt ggagccgctg gtggctggtc
    39961 gatccgttgg atggcaccaa ggaatttatc gccggcagcg aggagttcac cgttaacgtc
    40021 gcgctgatcg aacagggtcg cgtggtattc ggcgtggtcg gtattccggc caccggcttg
    40081 tgctattacg gcggcgccgg cctgggtgcc tggcgcgccg atgcggcggg tgtcagcgag
    40141 gaaatccgtg tgcgcctggc tccgcaggag gcgtttactc tggtggccag ccgtcgccat
    40201 tccagcccgg cgcaggaagc cttgctggcc ggtttgagcg aacgctttgg tgatctgcaa
    40261 ctggccagtg tcggcagttc gctgaagttc tgcctattgg ccgagggcaa tgccgattgc
    40321 tacccgcgtt tggcaccgac ttcgcagtgg gacaccgcag cggcgcaggg tgtgctcgaa
    40381 ggtgcgggtg gtgaagtgct gacgctcaag ggcgaggcgc tgacctatga ggcgcgcgaa
    40441 tcgctgctta acccgtattt tctggctctg ccagcggccg ccgcctggcg cgccgagctg
    40501 attcagctgg cccgctcgct ggattaacac tttgctcgtc ggatgatggg tatcgctgcg
    40561 ctcaacctac cttacgttgc ccacgatctg taggagcggg ccatgcccgc gatagatttc
    40621 ggatctgatc gcgggcaagc ccgctcctac attctcggcg gtggttcggc ttactcgagc
    40681 atatcgccgt agcgttcgtc cttcttgaac accagcggct gggcaaagcc cggcaccttg
    40741 atttgctcgc tgctgatctc gatgcccttg tcgtcgatca ggtcgttgcc gaagttatag
    40801 ataatcccca gatcacccag cagcgcttgc gcgtcgtagg cctgggccgc cttgcgggtg
    40861 acttcgcggc gctgcaggta ggcggcaaac gcggcttcgc catggcgctt gcgcagcatg
    40921 cgttcagcca catgcggggc cagcaacagg gcgctggcgt tgttgccgcc aaaacccttg
    40981 gagttgagca cgcacagatc caactgctcg gatgcgcggg agcgatcctg ggtggcgaag
    41041 ctgaggtgct gttgatgcac gtcttcggct acgccgtcga tggtcttgat ccccgggatg
    41101 atgccgtact tgaaactgcc cagggccgag atcagctggt cgccgctggc ggtggccagg
    41161 gaatggccga caaaggcttt aaccgccgtc accggccact gctcgatggc gaaggcgctg
    41221 gccacgcggt cgagaatttc cgattcggtc acccggttgg ccggggtgct agagccgtgg
    41281 gcatgcacaa agctgcgttc acgtaccgcg tcgatgccca gcagttgtac ggcgctggct
    41341 acggccttgg ccacggtcag gtagttgccg gggccgggag cggagatgga tttcttgaag
    41401 ccgtcggcgt tgataaacac atccggcacc gcaccgtgga tatccgcgcc caactccagg
    41461 gccagctcat catccatcag cactacaaac tggcaggctt cggccagggt aaagccgcag
    41521 ttctcgccaa atgggcggct ggcgcggcgg aagtcgacgg tctcctttcc ttctatttgg
    41581 cgcaggccat cttcggtggc cagggcgccc atggcgccgt aaccctcgat gcattcttgg
    41641 ttgattggcg cttcgctgct gccgatgatg actacccgcg ctttgccatt ggcaatctgt
    41701 tcgatgcctt tctgcaggtt atagaggaag gtggcgcagg cgccggtgat ggcgccggtg
    41761 gtgccgacgc tgcccagcac ataggcgttg ataaagtctg ccgacatggt gttgaggcct
    41821 agggccagtt gcttggcggt gacgcgtccg cctttgaggc gcgactgcat caggccgccg
    41881 aaaccgtttt cgtcgagctg gctcatgatg caactggaga acaccgcgac ctcatcgggg
    41941 gccacgtgct gcatgatggt ttgccagtcg atgccggtgg cgcgtagcgc gtcggtgacg
    42001 ccgacgatgg tcatggccag gccgcgcgga tggaagcgcg cgttgtatag ctcgctcggc
    42061 tcgaagccgg taggcagctg accggcggat ttcaccggca gggcgcggta gctgtcgacc
    42121 ttgaattcgc aattgtcatg cagggtcacc agcacttcgc tggcgttcag ctcgtccacc
    42181 gtccaatcgg agggcagtgg gtccggcagg tgcttgcgca gagtgataaa actcagcggc
    42241 tggccggcgg tggcgctgat atcgatggtc ttctgccagt gcgccgcgtc gacatccagg
    42301 tgctgtttct cgatgcggcg taccagggtt gaagcgagaa tctgttcggc gaagcggctg
    42361 tcgatctcgg ccaggctcag ggtgttgccg gcgtcatcct gataatcgcc atcaaccacc
    42421 ttgaccagtt tcatcatcac cgccagaccg gccagggtct gctggcgcgc cgacgtgtcc
    42481 atggactcga ttacggtacg gcggaaacca tggtgaaacg agctgcgccc ggccgcgtta
    42541 tagccaccaa aaccaacgat cacaggtaag cgtgaggtca ctcgataact ccttgaaaag
    42601 agggcgccag acaagccgag ataaagctat ggaggcttgt cgcacaggct agagcgctct
    42661 gcggcggtta ttacgccgcg atcaagcgcc cagcatggcc gttcggatgc ctatgctgac
    42721 gtgattgctg acttgtaagt cacggtttcg accaaggtag cgcatcgcta aaaccctacg
    42781 gctgttatgg tttctccgta taaaggccca gctggagaac aacaatgcac agcgtcaatc
    42841 catacgaata cggcatgccc cgtgatgcgg caaattttca ggcgctcagc ccgctgagct
    42901 ttctcgagcg cgctgccagc gtttacccgc agcgcctggc gctgattaat ggtcgtctgc
    42961 gccagacttg gggcgaaacc tacagccgca gtattcgtct ggcctcggcc ctggctcagc
    43021 gcggcatcgg cctgggcgat acggtggcgg tgatcgcgcc caacggcccg gcgatgttcg
    43081 aggcgcactt cggtgtgcca atgtgcggcg ctgtgctgaa tgccatcaac acccgtctgg
    43141 atgccgaagc catcgctttt atcctgcagc acggcgaagc caaggtactg ctggtggaca
    43201 aggaatttgg cgagctggcc cagcgcgtgg tcggccattt gccgcagccg ccgctggtga
    43261 tcggcatcga cgatactgaa tatgccgagg gtctgctgat cggtcagctg gattacgaag
    43321 cgctactggc cgaaggcgac ccggactatg cctggcagtt gccggccgac gagtggcaag
    43381 cgatctccct caattacacc tcgggcacca ccggcaaccc caaaggcgtg gtctatcacc
    43441 atcgcggcgc gcacctgaat gcgctgtcca acgccatgtg ctgggacatg agccgcttcc
    43501 cggtgtacct gtggacgctg ccgatgttcc actgcaacgg ctggtgtttc ccttgggcgc
    43561 tggcggccta cgtcggggtc aatgtgtgcc tgcgccatgt acgcgccgag gccatctacc
    43621 cggcgattgc cgagcatggc gtcgaccact tctgcggtgc gccgatcgtg ctgaatatgc
    43681 tggccaacgc cgatgaggcg ctgaaagccc tgaaaaccca accggtaaaa gtgctcaccg
    43741 ccggcgccgc gccacccgct gcggtaatcg aagctatgga agccctggag tttcgcgtta
    43801 ctcacgttta cggcctgacc gaaacctacg gccccagcgt ggcttgcgag tggaaaagtg
    43861 aatgggacag tgaaagcccg gaacagcgcg cgcgcctgaa gtcccgccag ggcgtgcgtg
    43921 cgccgctgct cgacggcctg atggtggccg attccgatac cctgcaaccg gtgccgaaag
    43981 atggccagac catcggcgag atcatgatgc gcggcaacgt ggtgatgaag ggctacctga
    44041 agaacccgtc ggccaccgcc gaagcctttg ccggtggctg gttccactcc ggcgacctgg
    44101 cggtgtggca tgccgacggc tatgtggaga tcaaggatcg cagcaaggac atcatcattt
    44161 ccggcggcga aaacatctcc tccatcgagg tggaagacgt gctgtatcgc catccgctta
    44221 ttttggaagc tgccgtggtg gccatggccc acgccaaatg gggcgaaacg ccgtgcgcct
    44281 tcgttaccct taaaccgggt gccgagttga ccgccgagga ggtgatcgcc ttctgtcagc
    44341 agcgtatggc gcgcttcaag gtgccaggtt gcgtggtatt caccagcctg ccgaaaacca
    44401 gcaccggcaa ggtgcaaaag tttgtgttgc gcgagcaggc caagcaattg gccgcgcagc
    44461 aggcggtggc atgaaggagg attgaaggca cttgttacag gtttaaatag atagcgagtt
    44521 gagaagctaa ataccattca ctactattcg gcaccttaac tcgtctatac caagaggcat
    44581 tacaccatgc aggtaagcaa aggcttgttc gccgcactct ccctcaccgc gctcgccagc
    44641 actgcgcagg ccgccgatga agtggtggtt tattcctcgc gtatcgacga gctgatcaag
    44701 ccggtgttcg atgcctacac cgctaaaacc ggggttaaga tcaagttcat caccgacaag
    44761 gaagcgccgc taatcgcccg tctgaaagcc gaaggtgcca ataccccggc tgacctgctg
    44821 atcaccgtcg acgccggcaa cctctggcaa gccgagcagg aaggcgtgct gcagccgacc
    44881 aagtccgagc tgatcactgc caatatccca gcccagtacc gctccagcac cgacagctgg
    44941 actggcctgt cgctacgcgc gcggaccatc ttctactcca ccgagcgggt taaaccgagc
    45001 gagctgagca cttacgaagc cctggctgac aaaaactggg aaggtcgtct gtgcctgcgt
    45061 accagcaaga aggtttataa ccagtcgctg accgccaccc tgatcgaaac ccatggtgcg
    45121 gccaaaactg aagaaatcgt caagggctgg gtcaacaacc tggccaccga cgtgttcgcc
    45181 gatgacaccg cgctgctgca agccatcgac gccggccagt gcgacgtcgg catcaccaac
    45241 acctactact acggccgcct gcaccagcag cagcctgatc tgaaagtgaa accgtactgg
    45301 ccaaaccaga acgaccgtgg cgtgcacgtc aacctggccg gtgccggtgt gaccaaacac
    45361 gcgccgcacg ccgaagcagc acgcaagctg ctggagtgga tgaccactgc tgaagcgcag
    45421 accatcttcg ccggcgtcaa ccaggaattc ccggccaacc cggccgttga gccttctgcc
    45481 gaagtggcag cctggggtac tttcaaggcc gacaccatcg ccactgaagt ggccggcaag
    45541 cgccaggccg aagcgaccat gttgatggat cgcgctggct ggaactaaaa ccataacgat
    45601 ttgctgcgcg tcggccctgc ggcgttaaaa acaggctcgg aatgctcatg tacaaaagta
    45661 cactccgctt cctcgccgtt ttgaccagcc ggaggctgtt actaacgtag cgccttgcag
    45721 gactctagct cgcgaaatcg taaacaggtt ctacggccta cgccgttata ctcgacgccc
    45781 cggcttagtc cggggcgttg tgttttccag ttttgaggat tttgcgtggc ccatcctgcc
    45841 cagcgccgct ggtaccccat cgccattgcc gttgccttgc tggtgctgct gccgttgagc
    45901 gtgctgatgt tcagttggca tgaggtcgat cagcagatct gggcgcacct gtggcaaacc
    45961 cagttaccgc gtctgctcgg caatacccta gtgctggttc tcggcgttgg tattggcgtg
    46021 acgctgctcg gcgtcagcct ggcctggctc accagcctct gtgaattccc cggtcgtcgc
    46081 tggctggact gggcgttgat gctgccgttt gccattcccg cctatgtgct ggcgttcgtg
    46141 tttgtcggcc tgctggattt tgccggcccg ctgcagaccc tgctgcgcga gtggttcggc
    46201 agcgatgtgc gcttcccccg cgtgcgctcc accggcgggg tgatcatcgt gctggtgctg
    46261 gtgttctacc cctacgtcta cctgcttgcg cgcaatgcct ttctggccca gggcaagggt
    46321 ttgatggagg ccgcgcgggt gcttggcctg agcccctggc gagcgttctg gcgtgtggcc
    46381 ttgccgatgg cgcgcccggc gattggggcc ggcctggcct tggcgataat ggaaaccctg
    46441 gcggatttcg gcgcggtttc ggtgttcaac ttcgatacct ttaccaccgc gatctacaag
    46501 acctggtata gcttctacag cctgaccagc gccacccagt tggccagtct gctgctgctg
    46561 gcggtgatgc tggtgctcta tggcgagcgt cgcgctcgcg gtgcggtgcg tccggtgaat
    46621 gagcgggcac gcagcaaggc gttgtatcac ctcaagggcg gcaaggccct cgccgccagc
    46681 acctggtgcg gcctggtgtt cgcctgcggt tttgtgattc cggtgctgca gctgattgtc
    46741 tggttctggc agcgcggtcg tttcgatctg gatgagcgct acagcgcctt gatcctgcac
    46801 accctctacc tgggcgcgat ggcggcgctg atcaccgtca gcgtggcctt gttgctggcc
    46861 ttcgcccggc gtatggcgcc gacccggctg atgcgttcaa ccgtcggtgt ggccaacctc
    46921 ggttatgccc tgccgggctc gatgctggcg gtggcgatca tgctggcgtt cagttacctc
    46981 gaccgcgaac tggtgattcc actgtccagc tggctgggcg gtgcaggcaa gccggtgctg
    47041 ctcggcagcc tttcggcgct gctgttggct tatatgatcc gctttatggc ggtggcctac
    47101 ggcccgctgg aaaacagcct ggcgcgcatt cgcccatcct tgccggaagc ctcgcgcagc
    47161 cttggcgtcg gcggcgtcgg attattcttc aaggtctacc tgccgctgct ggtgccgggg
    47221 gcgctgtcag cggcgctgct ggtatttgtc gatgtgctca aggaaatgcc cgccaccctg
    47281 ctgatgcgcc ccttcggctg ggacacgctg tcggtgcggg tgtttgaaat gaccagtgaa
    47341 ggcgagtggg cgcgcgccgc actgccggcc ctgacgctgg tgctggtcgg cctgttgccg
    47401 gtgattctgc ttattcgccg ttcggcgcgg cgccacggct aacaaagcct gcttggcgcg
    47461 accgcctgcc atgctgggat gaccgccccc ggtgttgcgg ctacaatgcg cgccattcgc
    47521 agcggccgat catcttgata gcccgtgcca gtgccctgtt tccaagggct cagcaaggtt
    47581 gccgctgcac tgcccagccc gcaaggagac acttatggga cagcgcacac cgctctatga
    47641 cctgcacctc gccttgggtg ccaagatggt cgacttcggc ggttgggaca tgccgttgca
    47701 ctatggctcg caggtcgagg agcaccatca ggtgcgtcgc gactgcgggg tgtttgatgt
    47761 gtcgcacatg accgtggtgg atgtcagcgg cagccaggcc aaggcctatc tgcaacacct
    47821 gctggccaac gacatcgagc gcctgcagag caacggcaaa gccctctaca gcggcatgct
    47881 caacgagcag ggcggggtgg tggatgacct gatcgtctac ctcaccgact ttggctaccg
    47941 cgtggtggtg aatgcctcga cccgcgacaa ggacctggcc tggatgcgcc tgcagagcgt
    48001 cgactttgac gtgcagatgc aagagcgcgc cgagctggcg atgctggcca ttcaaggccc
    48061 gcatgcgcgc agcaagactg ccgaactggt cagccaatcg cgcgccaacc tgattcagag
    48121 cctcaaaccg ttcgaaggcc agcccgatgg cgactggttt atcgcccgca ccggctacac
    48181 cggcgaagac ggcctggaaa tcatgctgcc ggccagcgaa gtggtggcct tccttaatga
    48241 gctggtgggt gccgggattt ccccgatcgg cctcggggcg cgcgataccc tgcgcctgga
    48301 agccggaatg aacctgtacg gccaggacat ggacgaaagc gtcaccccgc tggcggccaa
    48361 tatggcctgg accattgcct gggagccggc cagccgtggt tttgttggcc gtgccgccct
    48421 gcagaagcag aaagccgaag gcgtcggctc caagctggtt ggcctggtgc tggaagagcg
    48481 cggcgtactg cgtgcgcacc aagtggtgcg ggttgaaggg gtgggtgaag gtgaaatcac
    48541 cagcggcagc ttttcgccga ccctgggtaa atccatcgcc ctggcccgcg tgccggcggc
    48601 taccactgaa cgcgccgagg tagagattcg cggcaagtgg tacccagtgc gggtggtcaa
    48661 accaacgttt gtgcgtaacg gcaaggcgct tatctaagcc ttgagctggc catggatggt
    48721 cgagaacgca ccgttttgca gcggttcgct agtttttaaa gactttcttt cggtatggcc
    48781 aaggcctgct aaatttccat gacggcgtgg tcgctgtcat gtcagatgag gaacgacaca
    48841 tgagcaatat ccccgccgat ctgcgttacg ccgccagcca cgagtgggcg cgcctggaag
    48901 ccgatggcag cgttaccgta ggcatttccg atcacgcgca ggaagccctg ggtgat
//
LOCUS       DASRUM010000188        16893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_99722, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000188 DASRUM010000000
VERSION     DASRUM010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 16893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16893
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99722"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VFF22_12185"
     CDS             complement(<1..289)
                     /locus_tag="VFF22_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZX17602.1"
                     /translation="MSDSRTWAREAIRIIEADFQRSADTHLIPLSLPGLPGVELYFKD
                     ESSHPTGSLKHRLARSLFLYALCNGWLKPGAPVIEASSGSTAISEAYFARLL"
     gene            complement(300..1205)
                     /locus_tag="VFF22_12190"
     CDS             complement(300..1205)
                     /locus_tag="VFF22_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZX17603.1"
                     /translation="MREQGRGIAVICLVIAMALWGSSFIALKLAFAELPPLWVIFGRM
                     ALGSLVFLLAWRWRGQMHYRAGDWKYLLGLAACEPCLYFLFEALALQHTSAAQAGMVT
                     ALLPLLVAVGAFIFLRERISRITLAGFLLALIGAVWLSLAGSADEHASNPILGNFYEL
                     LAMLCAMGYTLLLKHLSSRYSPFLLTAMVAFVGTLFFLPLALFSAPLPTSVSPMGLGA
                     VAYLGILVTVGAYGLYNFGVSRLPASQASGFTNLIPVFTLLFAILLLGESLNAVQMLA
                     AGLVFAGVLLSQWRGTRVVPAGVLD"
     gene            complement(1290..1622)
                     /locus_tag="VFF22_12195"
     CDS             complement(1290..1622)
                     /locus_tag="VFF22_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit G"
                     /protein_id="HZX17604.1"
                     /translation="MDIWIEALVGLFLLLGSLFALVGAIGLYRLPDFFMRLHGPTKAS
                     TLGVGGMVIASLIYFSFRGEGVSLHELLIALFLFITAPVSAHMLAKAALQQKLRISEQ
                     TSGKPWEQ"
     gene            complement(1778..2047)
                     /locus_tag="VFF22_12200"
     CDS             complement(1778..2047)
                     /locus_tag="VFF22_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HZX17605.1"
                     /translation="MLAYVIPLCLVIIGLALVLTMARLIIGPDLPDRILALDTLYINA
                     IAMLVLFGIWLGSDLYFEAALLIAVMGFIGTVAVAKYLLRGDIIE"
     gene            complement(2032..2535)
                     /locus_tag="VFF22_12205"
     CDS             complement(2032..2535)
                     /locus_tag="VFF22_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HZX17606.1"
                     /translation="MKARRWLPHPLLSLCLLLVWLLLINDLSLGQWLLGALLGWAIPL
                     LTQVFWINPPRVYKPLKLTLFLLRVLGDIVIANLQVAKLILGPTAKLRPAFVEIPMLL
                     EDELALTMLTSIISLTPGTVSADLSDDRTMLLVHGLDVPDAEAMVAEIKQRYEAPLLE
                     VFTCSPT"
     gene            complement(2532..4037)
                     /locus_tag="VFF22_12210"
     CDS             complement(2532..4037)
                     /locus_tag="VFF22_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HZX17607.1"
                     /translation="MNHGLILPILLPMFAGSLLLLGAGLSLRVKRSLSLLATLLLLPL
                     SGYLLWLADQGVLQVYAAGNWAAPFGIILLLDRLSALLLVVTAVLGSFALLYAVRGDD
                     QRGQSFHALFQFQLMGINGAFLTGDLFNLFVFFEILLIASYALLVHGGGVERVRAGLH
                     YVVLNLVGSAFFLLAVGVLYGITGTLNMADMAIRVAAAGPDDAALLGAAGLLLLVVFG
                     LKAAFVPLYFWLPKAYASATAPVAALFAIMTKVGLYSIIRVYTLIFGDEAGSLANMAN
                     DWLWPIALLTLALGVIGALAAVSLQGLLAYLVVVSVGTLLAGVAIGTEQALAAALYYL
                     VHSTWVAGGLFLLADLIARQRGEKQALLVQGPALQNPHLLGALFFFGAIAVAGLPPLS
                     GFIGKLLLLRSLEPGLQALLLWPIVLGGGLLTLIALSRAGSMLFWRVGLSQLDSAELD
                     YGRLLACAGLLLLSLLLMIGAAPLLSYVEATASQLHDLALYRQIIEPGAAR"
     gene            complement(4034..4363)
                     /locus_tag="VFF22_12215"
     CDS             complement(4034..4363)
                     /locus_tag="VFF22_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HZX17608.1"
                     /translation="MEAIFAATLGILTASGVYLLLRARTFPVVLGLTLISYAVNLFLF
                     AMGRLQSGVATVIGVSEEYADPLTQALVLTAIVIGFAMTAFVVVLALRSVGETQTDHV
                     DGQEPVQ"
     gene            complement(4365..7157)
                     /locus_tag="VFF22_12220"
     CDS             complement(4365..7157)
                     /locus_tag="VFF22_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HZX17609.1"
                     /translation="MTLALIIALPFLGIFLPLLAERLGRSACAAAAALAPLTALVLLL
                     SQQSAVFAGDLLKFKLEWLPTLGLNLSLRLDGLGFLFALLILGIGLLVILYARYYLAK
                     KEPMGRFFAFLLLFMGAMLGVVLSENLLLMLMFWELTSLSSFLLIGFWGARTDARKGA
                     RMALAVTGGGGLALFAGILLIGHIAGSFELSQVLAAGYAIRAHELYPLALILVLLGVF
                     TKSAQFPFHFWLPHAMAAPTPVSAYLHSATMVKAGVFLLARLYPALAGSEWWFYLVSI
                     TGLVTLLVGAGMALFQHDLKGLLAYSTISHLGLITLLFGLDTRLAAVAAVFHIINHAT
                     FKASLFMAAGIIDHETGSRDMRRINGMWKYMPHTAVLAMVAASAMAGVPLLNGFLSKE
                     MFFTETLNQHLLGSFNWVIPAAATLAGVFSVAYSLRFIHDVFFNGEPIDLPHFPPHEP
                     PRYMKVPVEILVFLCLLVGIVPAYTVAPLLAAAAAATLGGEVPSYSLAIWHGFNLPLL
                     MSFIALFGGILVYVFRQPLFRWYAGLPSVDAKLLFERGVLILVKVCSVVTNWLENASL
                     QRYLALLLAAALLVVTQGLSSLPQISGPIPMTKIDGITALGLGIMALAALVTVVFHRQ
                     RLVSLLMLSVVGLMVALAFARYSAPDLALTQLSVEVVTIILLMLALFFLPAHTRIESS
                     SLRGLRDFTLAVGSGVMVAMLVFAVLTRPYDSISTFFLENSVPGGGGTNVVNVILVDF
                     RGFDTMGEITVLAIAAVGIFALLDGLRLYQPKVDAQGRSWARDRHPLILATLSRVLLP
                     MALLVSVFIFLRGHNLPGGGFIAGLVTAVALILQYVASGVQWTQSRLPLNYQGMAGLG
                     VLVAGLTGLGSWLFDRPFLTSAFGHFHIPLIGEIELATAMLFDLGVYLTVVGATLLIL
                     ANLGKLTQEKAAQEVL"
     gene            complement(7482..7943)
                     /locus_tag="VFF22_12225"
     CDS             complement(7482..7943)
                     /locus_tag="VFF22_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HZX17610.1"
                     /translation="MRKPFLLAASFAALALLGGCASSLTGDTYSRDEARAVQTVRMGT
                     IEALRPVKIEGTKTPIGAGAGAVIGGVAGSGVGGGRGSAVAAVIGAVAGGLLGAMTEE
                     GLTRTQGVEITVREDDGSMRAYVQAVEENQIFRVGERVRIMTVNGTSRVTH"
     gene            8119..8355
                     /locus_tag="VFF22_12230"
     CDS             8119..8355
                     /locus_tag="VFF22_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009047374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17611.1"
                     /translation="MDYFIVAITSVAGLYFHWWLYVRIKRWMDRDLALSLAGNSAAKR
                     SYMLARLAEAQTAGIKRSALSAWLEQAAAQYSGD"
     gene            complement(8363..9010)
                     /gene="pdxH"
                     /locus_tag="VFF22_12235"
     CDS             complement(8363..9010)
                     /gene="pdxH"
                     /locus_tag="VFF22_12235"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HZX17612.1"
                     /translation="MTQTLADMRRDYTRDGLSEAQAPLEPFALFEHWFADAVKTEQVP
                     VEPNAMTLATVDGEGRPHCRVLLLKGLDARGFTFFSNYDSAKGQHLAANPFAAMTFFW
                     PSLERQVRIEGRVERVTAEECDAYFQVRPLGSRLGAWASPQSQVIADRAELEGLLAET
                     EQRFLDRAPHCPPHWGGYRLLPERIEFWQGRSSRLHDRLNFRLQNGTWLRERLAP"
     gene            complement(9100..9786)
                     /locus_tag="VFF22_12240"
     CDS             complement(9100..9786)
                     /locus_tag="VFF22_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007996800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZX17613.1"
                     /translation="MLANKTLAIALCVLISGCSLFEKSDKAAPKVVPMPPPAVTQAWL
                     DVYEPKVREAIKGTHFEFERRENLLVVTAPVQGSFNPDRPHMMLPTTLGPLSRMAKLL
                     ESDETIGVLILGHADSSGEVSANRELSHQRARAFTSIFRLSGLKQNRLMVKGLGSDMP
                     RAANDSQQGRALNRRVEILLTSKATLNALISKYSQPAPAPANAVAAAKPAADKSAKTV
                     AKADKPATSQ"
     gene            complement(9882..11027)
                     /locus_tag="VFF22_12245"
     CDS             complement(9882..11027)
                     /locus_tag="VFF22_12245"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017336808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZX17614.1"
                     /translation="MQIQGYFDLKFEALRDAFAALFDEPQERGMALCVQIGGETVLDL
                     WAGVADKDGQQAWHSDTILNLFSCTKTFTAVTALQLVGEGKLELDAPVARYWPEFAAA
                     GKDKITLRHLLSHQAGLPALRQMLPAEALYDWQAMTTALAAEQPWWVLGEGHGYAPIT
                     YGWLVGEVLRRVEGRGPGESIVARTAKPLGLDFHVGLADAEFDRVAIISRGKGNFGDA
                     AAQRLLKTMMSEPAAMSTRAFTNPPSIMTSTNKPEWRRMQQPAANGHGNARSLAGFYS
                     GLLDGQLLESELLAELTREHAVGEDKTLLTRTRFGLGCMLDQPEVANATYGMGPRAFG
                     HPGAGGSIGFADPERDVAFGFVTNNLGPFVLMDPRAQKLARLLADCL"
     gene            complement(11144..13279)
                     /locus_tag="VFF22_12250"
     CDS             complement(11144..13279)
                     /locus_tag="VFF22_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-agarase"
                     /protein_id="HZX17615.1"
                     /translation="MATEQQELFNFVRPMDAVQVTTQDADLPNRIGEPTPQGEVLRRV
                     TFHPAEQPSLRLTPQNASWDWSQQTLVSLRVQNAMDWALTLDVLIESTDGQRLSTRIA
                     LPAGPAQTLLIPLQAASPRDQGMRAGVPMPWTHKGQRLLLADTVTGALDLSKVSAVTL
                     SIPQPKAAQHILISQFGVTSEQLTAAAYAGIVDSYGQYARADWPGKVKSDEQLQQAVA
                     QEQKQLHTWLAERPGQDKFAGWTGGMPFEASGFFRTEKRDGRWYLVTPEGHPFYSLGV
                     NAVTAQQSATYVEGREAMFAGLPQPGEALAAYFGTSDSRSDTGANQGRAFASGRWFDF
                     YKANLQRSYGQVDPAAWRTLSQDRLQAWGFNTLGNWSDPAFGGDTRMPFSIPLSIHGD
                     YATISTGVDWWGAMPDPFDPRFAMATERAVAIASRDHRDNPWLLGYFADNELAWAGPA
                     DDPNGRYALAYATLRLTTDVPAKRAFLKQLRDKYRNQNGLSRAWGIELPAWELMEDPG
                     FKAPLPSAEFPAIEKDMQAFLRLYADTYFKTIADSLDWHAPNHLLLGGRFASSIPEAV
                     NACAEFCDVLSFNFYTHEPQHGYDFAALRQLDKPLMVTEFHFGSRDRGPFWGGVAEVY
                     KEEERGPAYAHFLAKALEEPQIVGLHWFQYLDQPVTGRLLDGENGHLGLVGITDRPWD
                     GFVKAVRNANLDVPKTLLKPVPAPVPAAQ"
     gene            complement(13495..15639)
                     /gene="dinG"
                     /locus_tag="VFF22_12255"
     CDS             complement(13495..15639)
                     /gene="dinG"
                     /locus_tag="VFF22_12255"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HZX17616.1"
                     /translation="MLSTELKSQIQGAYSRFLEAKSLKPRYGQRLMIAEIAKVLGAIK
                     TDDEGRRLGDPAVVAVEAGTGTGKTVAYSLAAIPTAKAAGKRLVIATATVALQEQIVH
                     KDLPDLMRNSGLNFSFALAKGRGRYLCLSKLDILLQEGQAQSATAQLFEEEGFKIDVD
                     EQSQKLFTAMIEKLAGNKWDGDRDSWPEELEDTRWSQLTTDHSQCTNRHCPNFQQCAF
                     YKAREGMGKVDVIVTNHDMVLADLALGGGAVLPDPRDTLYVFDEGHHLPDKAIGHFAH
                     FTRLRSTADWLTQIDKNLTKLLAQNPLPGDLGRLIEQVPELARELKTQQQFMFAACEQ
                     VADFKPGEDMQGRERPRHRFVGGVVPEHLIELGLELKKGFSKLNDLFTGVTEQLKEAM
                     DGEGAVGIASHQAEEWYPLFGSLLARAKGNWELWLAFTAEDPEESPPMARWLTLADSG
                     ALFDIEVNASPILAAETLRRNLWNVAYGALVTSATLTALGTFDRYRMRAGLPKVAVTT
                     VVPSPFHHADAGVLRVPNLNADPRDAVAHTAAIIRDLPGLVEGSRGTLVLFSSRKQMQ
                     EVFDGLERDWRKRVFIQGNLSKQETLNKHKARVDSGEESVLFGLASFAEGVDLPGAYC
                     EHVVIAKIPFAVPDDPVEAALAEWIEARGGNPFMEIAVPDASLRLVQACGRLLRTEAD
                     RGTITLLDRRVVTQRYGKAILNALPPFRREIS"
     gene            15832..16296
                     /locus_tag="VFF22_12260"
     CDS             15832..16296
                     /locus_tag="VFF22_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011059620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HZX17617.1"
                     /translation="MTPYAFIYSLHLLAALIWVGGMFFAWMILRPAAVAALEAPPRLK
                     LWLEVFPRFFYWVWAAVIVLPVTGVGMLHLRFSGFDAAPRYVHIMMGLYIAMLALFLR
                     IQALQLPELRRAVAAEDWPNGGAVLGRIRRLVGINLLIGILVVAIAGIRPSF"
     gene            complement(16350..>16893)
                     /locus_tag="VFF22_12265"
     CDS             complement(16350..>16893)
                     /locus_tag="VFF22_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HZX17618.1"
                     /translation="AIAPGDQVLRLEIETADIASGAQISARGARGSAEKPALPGRNLS
                     LRLQAVTLENLVLDARGGTGAPGYAGLAGADGKPGGCTWGEASRGHDGLNGGDGQPGA
                     AGAQVRLEVPQGFHIEQLQVRLDGGSGGQPGAAGAGGQGGVSKGCWLYSTDGARDGKP
                     GQAGRAGEPGPAGALDIVQF"
BASE COUNT         3653 a         5404 c         4968 g         2868 t
ORIGIN      
        1 ccagtaagcg ggcgaaatag gcctcggaaa ttgccgtgga accactggat gcctcgatca
       61 ccggtgcgcc gggtttgagc cagccgttac atagcgcata gagaaacagc gaacgcgcca
      121 ggcggtgctt aaggctgccg gtgggatggc tggattcgtc cttgaaatac aactcgacgc
      181 ccggcaaacc tggcagcgac aggggaatca ggtgggtatc ggcgctgcgc tggaaatccg
      241 cctcgatgat gcggatcgcc tcgcgcgccc aggttcttga atcactcatg ccttaccccc
      301 taatccaaaa caccggccgg caccacacgc gtcccgcgcc actgactcag caatacgcca
      361 gcaaacacca atcccgctgc cagcatctgc acagcattca ggctttcgcc caatagcagt
      421 atggcgaaca gcagggtgaa caccgggatc aggttggtaa agcctgacgc ctgactggcc
      481 ggcaagcggc tgacgccgaa gttgtagagc ccgtaagcgc ccaccgtgac caatatgccc
      541 aggtacgcca ccgcgcccag gcccatggga ctgacgctgg ttggcaacgg cgcgctgaaa
      601 agtgccaggg gcagaaagaa aagggtgccg acaaaggcca ccatggcggt cagcagaaag
      661 ggcgaatagc gcgatgacag gtgcttaagc agcagggtgt accccatggc gcaaagcatc
      721 gccagcagct cgtagaagtt gccaagtatc gggttgctcg cgtgttcatc ggcgctaccg
      781 gccaggctta gccatacagc gccgatcagt gcgaggagaa atcccgccag ggtgatgcga
      841 ctgatccgct cgcgcaggaa gataaacgcc cccaccgcca ccagcagcgg caacaaggcg
      901 gtgaccatac ccgcctgagc ggcgctggtg tgttgaaggg ccaaggcttc gaagaggaaa
      961 tacagacagg gttcgcaggc ggccaggccg agcaggtatt tccagtcacc cgcgcggtag
     1021 tgcatctgcc cacgccaacg ccaggccagc agaaacacca ggctgcccag ggccatacgg
     1081 ccgaagatca cccacagcgg cggcagctcg gcaaaggcca acttgagcgc aataaaggaa
     1141 ctcccccaca gcgccatggc aatcaccagg cagatgaccg cgatgcccct tccctgctca
     1201 cgcataccca agccccgaac taaaggctgc agtgtagaga ggccggcgcg gcgccgacag
     1261 atacagtcag tgccgcgaac tgtgtgggct tattgctccc agggcttacc gctggtctgc
     1321 tcactgatcc gcagcttctg ctgcagcgcg gctttggcca gcatatgggc gctgaccggg
     1381 gcggtgataa acagaaacag ggcgatcagc agttcgtgca ggctgacgcc ctcgccgcga
     1441 aagctgaagt agatcagcga ggcgatcacc atgccgccca cgcccagggt gctggccttg
     1501 gtaggaccgt gcaggcgcat aaagaagtcc ggcaggcggt acaggccaat cgcgccgacc
     1561 aaggcgaaca agctgccgag cagtagaaac agccccacca gcgcttcgat ccagatatcc
     1621 attcagcaat ctccgtagaa cgtttttacg atctcgcgag ctagagccat gcaaggcaag
     1681 aacaggcgag gatgcggagt ttacgagctg taaatgagca atccgagcct gttcttaacg
     1741 cagcagggcc gacgcgcagc agatcgtaaa ctcgttctca ttcgatgatg tcgccgcgca
     1801 gcaaatactt ggccacggcc accgtgccga taaagcccat caccgcaatc agcagggccg
     1861 cttcaaaata caggtccgag cccagccaga tgccgaacag caccagcatg gcaatcgcgt
     1921 tgatatacag ggtatccagg gccaggatgc ggtcgggcag atcggggccg ataatcaggc
     1981 gcgccatggt cagcaccagg gccaggccga tgatcaccag gcacagcgga atcacgtagg
     2041 cgagcatgtg aatacctcca gcaagggtgc ttcgtagcgc tgtttgattt ccgccaccat
     2101 ggcctcggcg tccggcacat ccaggccgtg caccagcaac atcgtgcggt catcgctaag
     2161 gtcggcggac accgtgcctg gggtcaggga gatgatgctg gtgagcatgg tcagggccag
     2221 ctcatcttcc agcagcatgg gaatttccac gaaggccgga cgcagcttgg cggtcgggcc
     2281 gaggatcagc ttggccacct gcaggttggc aataacgata tcgccgagga cgcgcagcag
     2341 gaacagagtc agcttcagcg gtttgtagac ccgtggtggg ttgatccaga acacctgggt
     2401 caacaacgga atcgcccagc cgagcaacgc accgagcaac cattggccca ggctcagatc
     2461 gtttatcagc aacagccaga ccagcagcag acacaggctc agcaacggat gaggtaacca
     2521 acgacgtgct ttcatcgtgc agctcccggt tcgatgatct gccggtacag cgccaagtca
     2581 tgcagctggc tggccgtggc ttcgacataa ctgagcagcg gcgcggcacc gatcatcagc
     2641 agcaagctga gcagcagcaa gccggcgcag gccagtaacc ggccgtaatc cagctcggcg
     2701 ctgtccagct ggctcaggcc gacacgccag aacagcatgc tgccggcacg gctcagggca
     2761 atcagggtca gcagaccgcc gccgagcaca atcggccaca gcagcagcgc ctgcaaaccc
     2821 ggctccagcg agcgcagcaa cagcagcttg ccgataaagc cggagagcgg cggcaggccg
     2881 gctacggcaa tcgcgccgaa aaagaacagg gcgccaagca gatgcggatt ctgcaacgcc
     2941 gggccctgca ccagtagcgc ctgtttttcg ccgcgttgac gggcaatcag gtcggccagc
     3001 aggaacagcc cgccggccac ccaggtgctg tgcaccaggt aatacagggc ggcggccagg
     3061 gcctgctcgg tgccgatggc aacaccagcg agcaacgtac cgaccgacac caccaccaga
     3121 taggccaaca gcccctgcag actgaccgcc gccaacgcac cgatcacgcc cagcgccagg
     3181 gtcagcaggg caatcggcca gagccagtcg ttggccatat tggccaggct gccggcctcg
     3241 tcgccaaaga tcagcgtata cacgcggatg attgaataca ggccgacctt ggtcatgatc
     3301 gcaaacagcg cggcgaccgg cgcagtggcc gaggcatagg ccttgggcaa ccagaaatac
     3361 aacggcacaa atgccgcctt aaggccgaac actaccagca gcaatagccc agcagcgccg
     3421 agcagcgcgg cgtcatccgg tccggcagcg gctacgcgaa tggccatatc ggccatattc
     3481 agggtgccgg tgatgccata aagcacgccc accgcgagca ggaagaatgc cgaacccacc
     3541 aggttcagca ccacgtaatg cagccccgcg cgtacccgct ctaccccgcc gccatgcacc
     3601 agcagcgcat aggaggcgat caacaggatc tcgaagaaga cgaacaggtt gaacaaatcg
     3661 ccagtgagaa aggcaccgtt gatgcccatc aactggaatt gaaacagcgc atggaaactc
     3721 tgcccgcgct gatcatcgcc acgcaccgca tacagcaagg caaagctgcc gagcacagcc
     3781 gtgactacca gtagcaaggc gcttaggcga tcaagcagca agatgatacc gaagggtgcc
     3841 gcccagttac cggcggcata cacctgcagc acgccttgat cggccagcca gagtagatag
     3901 ccactcaacg gcagcagcaa caaggtcgcc agcagcgaca aactgcgttt gacccgcagg
     3961 ctcaggccag cgcctagcag caacaggctg ccggcaaaca tcggcagcag gattggcagg
     4021 atcaagccgt gattcattgc acaggctcct ggccgtcaac atggtcggtc tgggtttcgc
     4081 cgacgctgcg cagcgccagc accacgacga aggcggtcat ggcaaaaccg atcacgatag
     4141 cggtcaacac cagcgcctgc gtcaacgggt ctgcgtactc ctcactcacc ccgataaccg
     4201 tggccacgcc gctctgcaaa cggcccatgg caaacaagaa caggttgacc gcataagaaa
     4261 tcaacgtcag ccccagcacg accgggaagg ttcgcgcgcg cagcagcagg taaacaccgc
     4321 tagcggtcag gatgcccaag gttgcggcaa atatggcttc catctcaaag cacctcttgt
     4381 gcggcttttt cctgggtcag cttgcccagg ttggcaagaa tcagcagggt cgcgccgact
     4441 accgtcaggt acacgcccag gtcgaacaac atggccgtgg ccagctcgat ttcaccgatt
     4501 agcgggatat ggaaatgacc aaaggccgag gtcaggaacg gtcggtcaaa cagccaactg
     4561 cccaggccgg tcaggccggc aaccaacacg ccgaggccgg ccataccctg ataattcagc
     4621 ggcaagcgtg actgcgtcca ctgcacgcca ctggcgacgt attgcaggat caacgccacg
     4681 gctgtcacca gcccggcgat aaagccgcca cccggcaggt tgtggccgcg caggaagata
     4741 aacaccgaga ccagcagcgc catcggcagc agtacccgcg acaatgtggc gaggataagc
     4801 gggtgacgat cacgcgccca actgcgccct tgcgcgtcga ccttcggctg gtacaggcgc
     4861 aagccatcga gcagcgcgaa gatgcccacg gcagcaattg ccagcacggt aatttcgccc
     4921 atggtatcga agccgcggaa gtccaccaga atcacgttga ccacgttggt accgccgcca
     4981 cccggcacgc tgttttccag gaagaatgtc gagatactgt cgtatggccg cgtcagcacg
     5041 gcgaacacca gcatcgccac catcacccca ctgcccaccg ctaaggtgaa gtcgcgcagg
     5101 ccgcgcaggc tgctcgactc aatgcgggta tgcgcaggca ggaagaacaa cgcaagcatc
     5161 agcaggatga tagtcaccac ctctaccgac agctgggtca gggccagatc cggcgccgaa
     5221 tagcgggcga aggccagggc caccatcaat ccgaccacgc tgagcatcag cagcgaaacc
     5281 aggcgctgac gatggaagac caccgtcacc aatgcggcca gggccatgat gcccagcccc
     5341 agggcggtga tgccgtcgat cttggtcatc ggtatcggcc cgctgatctg cggcaacgag
     5401 ctcagaccct gagtcaccac cagcaatgct gccgccagca gcagcgccag gtagcgctgc
     5461 agcgaagcgt tctccagcca gttggtgacg acgctgcata ccttgaccag gatcagcacg
     5521 ccgcgctcga acagcagctt ggcatccaca ctcggcagcc cggcatacca gcgaaatagc
     5581 ggctgacgga acacgtacac cagaatcccg ccgaacaggg cgataaagct catcagcagc
     5641 ggcaggttga aaccgtgcca gatcgccaga ctgtagctcg gtacctcacc gcccagggtt
     5701 gccgcagcgg cagcagccag cagtggcgct acggtgtagg ccggaacaat ccccaccagc
     5761 aggcagagga acaccagaat ctccaccggc accttcatat agcgtggcgg ctcatgcggc
     5821 gggaaatgcg gcaggtcgat tggctcaccg ttgaagaaca cgtcatggat aaagcgcagc
     5881 gaataagcca ccgagaacac cccggccagg gttgccgccg ccggaatcac ccagttgaag
     5941 ctgcccaaca ggtgctggtt aagcgtctcg gtaaagaaca tttccttgct caggaagccg
     6001 ttgagcagcg gcaccccggc catcgccgag gccgccacca tcgccagcac ggccgtatgc
     6061 ggcatgtact tccacatacc gttgatccgc cgcatatcgc ggctaccggt ttcgtgatcg
     6121 atgatccccg ccgccataaa cagcgaagcc ttgaaggtgg cgtggttgat gatatggaac
     6181 accgcagcca ctgcggccag acgggtgtcc agaccgaaca gcaaggtgat caggcccaaa
     6241 tggctgatgg tcgaataggc cagcaggccc ttgaggtcat gctgaaacag cgccatcccg
     6301 gcaccgacca gcaaggtcac caggccggtg atgctgacca ggtagaacca ccactccgaa
     6361 cccgccagcg ccggatacag acgcgccagc agaaacaccc cggccttgac catggtcgcc
     6421 gagtgcagat aggccgacac cggcgtcggc gcagccatgg cgtgaggcag ccagaaatgg
     6481 aacgggaact gcgccgactt ggtgaacacc cccagcagca ccagaatcag cgccagcggg
     6541 tacagctcgt gggcgcgaat cgcgtaaccg gcagccagca cctgggacaa ctcgaaactg
     6601 ccagcgatat ggccgatcaa cagaatcccg gcaaacagcg ccaggccgcc accaccggtc
     6661 acggccagtg ccatacgcgc gcctttgcgt gcatccgtac gggcgcccca gaagccgatc
     6721 agcaggaacg acgacaggct ggtcagctcc cagaacatca gcatcagcag caggttttcc
     6781 gaaagcacca cgccgagcat cgcgcccatg aagagcagga ggaaggcaaa gaagcgcccc
     6841 attggctctt tcttcgccaa gtaataacgg gcgtagagaa tgaccagcag accaatgccg
     6901 agaatcagca gggcaaacag aaagcccaag ccatccagac gcaggctgag attcagcccc
     6961 agagtcggca accactccag tttgaatttg agcagatcgc cagcaaacac cgcagactgt
     7021 tgcgaaagca gcaacaccag cgccgtcagc ggcgccagcg cagctgctgc ggcgcaggcg
     7081 gaacggccca ggcgctcggc cagcagaggc agaaagatgc cgagaaatgg caaggcgatg
     7141 atcagcgcaa gcgtcataaa aaacccctta atacgaaccc ggcacgtacc cgtccccggc
     7201 tgactcccta acttgctact ggcggtcaac cacaggcttt tgcctgcgct gcacccgcgc
     7261 aaaagtattg caccacgcgg cgactaaagc ccgtcggcgc aaaagcgtgc gcgattatcc
     7321 ataactatcg gcaaaccgtc atggatagaa ttattactaa acactgtgtt ttccgcgaac
     7381 ttgcctgaaa aacccacaga acaccgataa acaactggtt tatctagaaa ataggcaata
     7441 aaaaaccgga tcagtcagaa ctgatccggc ttgttggaca ctcagtgggt aacgcggctg
     7501 gtgccattta cggtcatgat gcgcacgcgc tcaccgacgc ggaaaatctg attttcttcg
     7561 acggcctgta cataggcgcg catgctgcca tcatcttcgc gcacggtgat ctctacaccc
     7621 tgggtacggg ttagaccttc ttcggtcatc gcccccagca gaccacccgc cactgcaccg
     7681 atcaccgcag ccaccgcact gccacgcccg ccaccgacgc cactaccggc aacgccgccg
     7741 attaccgcgc cggcacctgc gccgataggg gttttcgtgc cttcgatctt cactgggcgc
     7801 aacgcttcga tggtgcccat gcgcacggtt tgcacggcac gcgcttcgtc gcgcgagtaa
     7861 gtgtcacccg tcaggctgga ggcgcagccg cccaacaggg ctagcgcggc aaacgaagcc
     7921 gccaatagaa agggtttacg catgatggtt tctccatttg ggcaggccct cattaaaacg
     7981 cctgcacacc gcagctgtca cccgccaata tgcagcaaaa ctttacccgc gctgcagcgc
     8041 aggaccatgg cgcgacactt cgccacgtgc aaccgcagca tccaatcagc ctaagctaag
     8101 acttagcttg ggggatttat ggattacttt atcgtcgcca tcaccagtgt tgccgggctg
     8161 tatttccact ggtggctgta tgtacggatc aaacgctgga tggaccgcga cctggcgctg
     8221 tcactggccg gcaactcagc ggccaagcgc agctatatgc tcgcccgctt ggcagaggcg
     8281 caaaccgccg gcatcaagcg cagcgccctg tccgcctggc tggagcaagc cgccgcgcag
     8341 tactccggcg actgaagcgc actcagggcg ccagacgctc gcgcagccag gtgccgttct
     8401 gcaaacggaa attcagacga tcatgcagac gactggagcg gccctgccag aactcgatgc
     8461 gctccggcag caggcgatag ccaccccagt gcggcgggca atgcggcgcg cgatccagaa
     8521 aacgctgttc ggtctcggcc agtaggcctt ccagctccgc gcggtcggca atcacctggc
     8581 tctgcggcga agcccaggcg cctagacgac tgcccagagg gcgcacctga aagtaggcat
     8641 cgcactcttc ggcagtaacc cgctcgacac gtccttcgat ccgcacctgg cgctccaagc
     8701 tcggccagaa gaaggtcatg gcggcaaaag ggttggcggc cagatgttga cctttggcgc
     8761 tgtcgtagtt gctgaagaag gtaaaaccgc gcgcatccag acctttaagc agcagcaccc
     8821 gacagtgcgg gcgcccttcg ccatcgacgg tcgccagggt catggcattg ggttcgaccg
     8881 gcacttgctc agtcttcacc gcatcggcaa accagtgctc gaacagcgca aacggctcaa
     8941 gcggcgcttg cgcctcgctc aagccatcgc gggtgtagtc acggcgcata tcagccaggg
     9001 tttgagtcat cggcaaatcc tctcgatcaa gtattgccta gcttacccgc gagcgctcta
     9061 tctggcttga tctgcggcaa cacgccgcta cgtcgcgcgt tactgactcg tggcaggctt
     9121 atcagccttg gctacggttt tcgcgctctt gtcagcggca ggcttggcag cggcgactgc
     9181 attggcaggc gcaggtgctg gctggctgta cttggagatc agcgcgttga gggtggcttt
     9241 agaggtcagg aggatttcca cccggcggtt cagcgcacgg ccttgctgac tgtcgttggc
     9301 ggcgcgtggc atgtccgagc ccaggccttt aaccatcaga cggttctgct tgagaccgct
     9361 gaggcggaaa atcgaggtaa aggcgcgggc gcgctggtgg ctcagctcac gattggcact
     9421 tacttcaccg ctgctgtcgg cgtggccgag aatcagcaca ccgatggttt catcgctttc
     9481 cagcagcttg gccatgcggc tcagcgggcc gagggtggtc ggcaacatca tatgtggacg
     9541 gtccgggttg aacgaaccct gcacaggcgc agtgaccacc agaaggtttt cccgacgctc
     9601 gaattcgaaa tgggtgccct tgatcgcttc acgcaccttc ggctcgtaca catccaacca
     9661 agcctgggtc actgccggtg gcggcattgg caccactttc ggcgcagcct tatcggactt
     9721 ttcgaacagg ctgcatccgc tgatcagtac acacagggcg atggcgagag ttttgttggc
     9781 gagcatcgga tatccacgtc gtgataagca aaaaaaggcc ggtcaatggc ccgctcagac
     9841 ggcaggccat gccatgttgc gcagcagttt agccgagctg gctataaaca atcagccaat
     9901 agacgcgcaa gtttttgcgc acgggggtcc atcaagacga acggtcctag gttattggtg
     9961 acaaaaccga aggctacatc ccgttccggg tcggcaaaac caatggaacc gccggctcca
    10021 gggtgaccaa aggcgcgcgg acccataccg taggtggcgt tggcgacctc gggctggtcg
    10081 agcatgcaac ccagaccgaa gcgggttcgg gtcagcaggg tcttgtcttc gcccactgcg
    10141 tgttcacggg tcagctctgc cagcaactcg ctttccagca actggccatc cagcaggcca
    10201 ctgtagaaac cagccaggct acgggcattg ccgtggccgt tagccgctgg ctgctgcata
    10261 cgccgccact cgggcttatt ggtgctggtc atgattgacg gcgggttagt gaaggcccgc
    10321 gtgctcatgg ccgcaggctc gctcatcatg gttttgagca ggcgctgggc tgcggcatcg
    10381 ccgaagttac ccttgccgcg agagatgatc gccacacggt cgaactctgc atcggccaga
    10441 ccgacatgga aatccagccc taagggcttg gccgtacgcg cgacgatgga ttcgcccggc
    10501 ccgcgcccct ctactcggcg caacacctca ccgaccagcc agccataggt gatcggcgca
    10561 taaccatggc cctcacccaa aacccaccag ggctgctcgg cggccaatgc cgtggtcatg
    10621 gcctgccagt cgtataaggc ttcggccggc agcatctggc gcaaggctgg cagcccggcc
    10681 tgatggctga gcaagtgacg cagggtgatc ttgtccttgc cggctgcagc gaactcgggc
    10741 cagtagcgcg cgaccggcgc atccaattcg agcttgccct cgcccaccag ttgcagcgcg
    10801 gtaactgcag taaacgtctt ggtgcaggaa aacaggttaa ggatggtgtc gctgtgccag
    10861 gcctgctggc catccttgtc ggcaaccccc gcccacaggt cgagcacagt ttcgccgccg
    10921 atttgcacgc agagcgccat gccgcgctcc tgcggctcat cgaacaatgc cgcaaaagcg
    10981 tctctcagcg cctcgaactt gaggtcgaaa taaccctgta tctgcaccac cctgccccct
    11041 tcttctactt ggccaaatgt tcggcagatt gtttcagctc taacgcctgc ggtgaaccct
    11101 gcacaaggct ggcaacggca ctatcagctc actgaatagc tcttcactga gccgcaggaa
    11161 ctggcgccgg tacaggctta agcagcgttt tcggcacatc caggttggca ttgcgcacgg
    11221 ctttgacgaa gccatcccac ggccggtcgg tgatcccgac caggcccagg tggccattct
    11281 cgccatccag caaacgcccg gttaccggct gatcgaggta ctggaaccag tgcagaccga
    11341 cgatctgcgg ctcttccagg gccttggcca ggaaatgcgc ataggctggg ccgcgctctt
    11401 cttccttata gacttcggca accccacccc agaacgggcc acggtcacgc gagccgaagt
    11461 ggaactcggt gaccatcagc ggcttatcca gctggcgcag tgcggcaaag tcataaccgt
    11521 gctgcggctc gtgggtgtag aagttgaagc tcagtacatc gcagaactcg gcgcaggcat
    11581 tcaccgcctc ggggatgctg ctggcgaaac ggccgcccaa gagcaagtgg ttgggcgcgt
    11641 gccagtcgag cgagtcggca atggttttga agtaggtgtc ggcatacagg cgcaagaatg
    11701 cctgcatgtc cttctcgatg gccggaaact cggcactggg cagcggcgcc ttaaaaccag
    11761 gatcttccat cagttcccag gccggcagct cgatgcccca ggcgcgggac aggccattct
    11821 ggttacggta cttgtcgcgc agctgcttga ggaaggcacg cttggccggc acatcggtgg
    11881 tcaggcgcag cgtggcgtac gccagggcgt aacggccatt agggtcatcc gcaggaccgg
    11941 cccaggccaa ttcgttatcg gcgaaataac cgagcagcca agggttgtcg cggtgatcgc
    12001 gcgaggcaat cgccacagcg cgctcggtgg ccatggcaaa gcgcgggtcg aacggatcag
    12061 gcatcgcgcc ccaccaatcg acgccggtac tgatggtggc gtaatcacca tgaatcgaca
    12121 gcggaatgct gaacggcatg cgcgtatcgc cgccaaaggc cggatcgctc cagttcccca
    12181 gggtgttaaa gccccaggcc tgcaggcggt cctggctcag cgtgcgccag gctgcggggt
    12241 caacctggcc gtagctgcgc tgcaggttgg ccttgtagaa atcgaaccag cgcccgctgg
    12301 caaacgcccg accttgatta gcaccggtat cgctgcggct gtcggaggtg ccgaaatagg
    12361 ccgccagcgc ttcacccggc tgcggcaacc cggcaaacat agcctcgcgc ccttcaacgt
    12421 aagtggcgct ctgctgcgca gtgactgcat tgacgcccaa cgagtagaac ggatgacctt
    12481 caggcgttac caggtaccag cggccatcgc gcttctcggt gcgaaagaag ccgctcgcct
    12541 cgaacggcat cccaccggtc cagccagcaa acttatcctg accaggacgc tcggccagcc
    12601 aggtatgcag ttgtttctgc tcctgggcca cagcctgctg caactgctcg tcactcttta
    12661 ccttgcccgg ccaatcagcc cgcgcgtact ggccgtaact gtcgacgatg ccggcatagg
    12721 cagccgccgt cagttgctcg ctggtcacgc caaactggct gatcagaata tgttgcgcgg
    12781 ctttcggctg cggtatggac agggtgaccg cgctgacctt gctcaggtcc agcgcacccg
    12841 tgaccgtatc cgccagcagc aggcgctggc ctttgtgcgt ccagggcatc ggtacgccgg
    12901 cacgcatgcc ctgatcgcgg ggcgaggccg cttgcaaagg aatcaacagg gtttgtgcag
    12961 ggccggcagg cagggcgata cgcgtgctca gtcgctggcc gtcagtgctc tcgatcagca
    13021 catccagggt cagcgcccaa tccatagcgt tctgtactcg caggctgacc agcgtctgct
    13081 gcgaccagtc ccagctagcg ttttgcgggg tcaggcgcag actcggctgt tcggcgggat
    13141 ggaaggtcac ccggcgcagc acttcacctt gcggggtcgg ctcgccgata cggttaggta
    13201 gatcggcatc ctgcgtcgtg acctgcacgg catccatcgg ccgaacaaag ttgaacagtt
    13261 cctgctgctc tgtggccatg gccgccccgt aaaaacccac gagcgctgat accagaacag
    13321 ccgtttgcag cctgcgctta accttcacga accacactcc cccattgttt caaccgcctt
    13381 gggcaatact gcccgaggca aaatccagac cctggtgatg accaaggcat tgtgcatgcg
    13441 ccagttggag ccactacagc gcggcgagtt gcgctttacc gacagacggg gcatttagct
    13501 gatttcccgc cgaaacggcg gcaaggcatt aagaatcgcc ttgccgtagc gctgagtaac
    13561 cacgcgccga tccaacaggg taatagtgcc gcgatccgct tcggtacgca gcaatcgacc
    13621 acatgcctgc accagacgca gcgaagcatc cggcacggcg atttccataa aggggttgcc
    13681 gccgcgcgct tcgatccact cggccagggc cgcttccacc ggatcgtccg gcacagcgaa
    13741 ggggatcttg gcgatcacca catgttcgca gtaggcgccc ggcaggtcga caccttcggc
    13801 aaaactggcc aggccaaaca gcacgctttc ctcgccggaa tcgacccgcg ccttgtgctt
    13861 gttcagggtt tcctgtttgg acaggttgcc ttggataaac acccgtttgc gccagtcgcg
    13921 ctccaggccg tcgaagactt cctgcatctg cttgcgcgag gaaaacagca ccagggtgcc
    13981 gcgcgaacct tcaaccaggc cgggcaagtc gcggatgatc gcggcggtgt gggccacagc
    14041 atcacgcgga tcggcattca ggttgggcac ccgcagcaca ccggcatcgg cgtgatgaaa
    14101 cgggctcggc accacggtgg taaccgccac tttcggcaaa cctgcgcgca tgcggtagcg
    14161 atcaaaggta cccaacgccg tcagggtcgc cgaggtaacc agggcgccat aggcgacatt
    14221 ccatagatta cgccgcaggg tttcggcggc aagaatcggg ctcgcattga cctcgatatc
    14281 gaacaacgca ccgctgtcgg ccagggtcaa ccaacgcgcc atcggcgggc tttcctccgg
    14341 gtcttcagcg gtaaaggcca gccacagctc ccagtttccc ttggcccgcg ccagcaggct
    14401 gccgaatagc gggtaccatt cctcggcctg gtgactagca ataccgacag cgccctcgcc
    14461 atccatggct tccttgagct gctcagtcac cccggtgaac agatcattga gcttggaaaa
    14521 gccttttttc agttccagac ccagctcgat caggtgctcc ggcaccacac cgccgacaaa
    14581 gcgatggcgc ggccgctcgc ggccctgcat atcctcgccc ggcttgaaat cagcgacctg
    14641 ctcgcaggcg gcaaacataa attgctgctg ggttttcagc tcccgcgcca actccggcac
    14701 ctgctcgatc agcctgccta gatcccccgg cagcggattt tgcgctagca acttggtcag
    14761 attcttgtcg atctgggtca gccaatcggc agtcgaacgc aagcgggtga agtgagcgaa
    14821 atggccaatg gccttgtccg gcaggtggtg accttcatcg aatacgtaga gggtgtcgcg
    14881 cgggtccggc agcaccgccc cgccgccgag ggctaggtcg gccaggacca tgtcatggtt
    14941 ggtgacaatc acatcgacct tgcccatgcc ctcgcgggct ttgtagaagg cacattgctg
    15001 aaagttcggg caatggcgat tggtgcactg gctgtgatcg gtggtcagtt gcgaccagcg
    15061 cgtgtcttcc aactcttccg gccaactgtc gcgatcgccg tcccatttat tgccggcgag
    15121 tttttcgatc atcgcggtga acagcttctg gctctgctca tccacatcga tcttgaagcc
    15181 ttcttcctcg aacagctggg cggtggcgct ttgcgcctgg ccttcctgca acagaatatc
    15241 cagcttggat agacataaat aacgccctcg gcccttggcc agggcgaagc tgaaattcag
    15301 cccgctgttg cgcatcaggt ccggcagatc cttatgcacg atctgctctt gcagggccac
    15361 ggtggccgtg gcgatcacca gacgtttgcc tgctgccttg gcagtgggaa tcgccgccag
    15421 gctgtatgcc acggtcttgc ccgtaccagt gccggcctca accgccacaa cagcgggatc
    15481 acccaggcgc cggccctcgt cgtccgtctt gatcgcaccc agcaccttgg cgatttcagc
    15541 aatcatcagg cgctggccat aacgcggctt gagcgacttg gcttcgagaa aacgggaata
    15601 ggcgccctgg atctgggact tgagttcggt actgagcatg gggtcttggc gcgaaaaggc
    15661 tggataaatt ttcagtgttt gtaatcggcg gctatcatag cgcgctaatc gatccagcgc
    15721 tgaaccgctt aaaacctgtt tacgatctcg cgcgctcaat cctgtttcta ccgcaacagg
    15781 gccaacgctc agcagatcgg caatagattc ttaaccaaca ggagtgcctg catgacccca
    15841 tacgccttca tctacagcct gcacctgctc gccgccctga tctgggtagg tggcatgttc
    15901 ttcgcctgga tgatcctgcg cccggcagcc gtcgccgcac tggaagcgcc gcctcgcctg
    15961 aaactctggc tggaggtctt cccgcggttt ttctactggg tatgggctgc ggtgattgtg
    16021 ctgccggtga ccggcgtggg catgctgcac ctgcgcttta gcggcttcga tgccgcgcca
    16081 cgttatgtgc acatcatgat ggggctgtat atcgccatgc tggcgttgtt tctgcgaatt
    16141 caggccctgc aactgccgga actgcgccgc gctgtagcag cagaggattg gccgaatggc
    16201 ggcgccgtac ttgggcgtat tcgtcggctg gtcggtatca acttactgat agggatactg
    16261 gtggtagcaa tcgccggcat ccgcccgtcg ttctaactac gggcaaagta accgctgcac
    16321 gaaggcagcg gttggtacct cgcaacagcc tagaactgca cgatatccag agcgcctgct
    16381 gggcctggct caccggcccg cccagcctgc cctggcttac cgtcgcgcgc accatccgtg
    16441 ctgtacagcc agcacccctt gctcacgccg ccttgtccgc ctgcaccagc cgcgcccggc
    16501 tggccgccgc tgccgccgtc cagacgcacc tgcaactgct caatatggaa accctgcggt
    16561 acttccaggc gtacctgcgc cccagcagca cccggctggc cgtcaccacc attcaaacca
    16621 tcatgaccgc gactggcttc accccaggtg cagccacccg gcttaccatc ggcaccggca
    16681 agaccggcat aaccgggagc acctgtgccg ccgcgggcat ccagcactaa attttccagg
    16741 gtaacggcct gtagtcgcag actcaggttg cgtcccggca gcgccggctt ttcagcactg
    16801 ccgcgtgcgc cacgcgcgct gatctgtgcg ccactggcaa tatcggcggt ttcaatttca
    16861 aggcgcaaca cctgatcacc aggggcaatc gcc
//
LOCUS       DASRUM010000189        12407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2114649, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000189 DASRUM010000000
VERSION     DASRUM010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 12407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12407
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2114649"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..979
                     /gene="motA"
                     /locus_tag="VFF22_12270"
     CDS             128..979
                     /gene="motA"
                     /locus_tag="VFF22_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HZX17619.1"
                     /translation="MAKIIGIIVVLASVLGGFILSGGQFMALVHPFEILIIGGAALGA
                     FLQANPGSAFMHVFKKSLSMFSSRFTHAYYLDVLKLLYEILNKSRREGMMAIEADVED
                     PAASPIFSKYPGILKDQQMTAFICDYLRIMSSGNMAPHELEGLFDMEISSLKEEVEHP
                     AHAVAKVADGMPAMGIVAAVLGIVITMSILAEADNAEIGQKVGTALVGTFFGILASYG
                     FFGPLSAALEHDAKEELNVYEAIKAGLVASASGMPPSLAVEFSRKVLLPAHRPSFAEL
                     EQAMRGG"
     gene            988..2010
                     /gene="motB"
                     /locus_tag="VFF22_12275"
     CDS             988..2010
                     /gene="motB"
                     /locus_tag="VFF22_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HZX17620.1"
                     /translation="MENNQPIIVKRVKKVAGGHHGGAWKIAFADFATAMMAFFLVMWL
                     MTVATPEQKKLISGYFQDPVGFSESASPYVIDLGGTPTPSPDRTLNDQPQEEQSPAED
                     AQADVDKLQAESIAEEVERERLELLLQELQNKVEENPQLQNFKDQILFEITQDGLRIQ
                     IMDAENRPMFASGSARLQPYFEDILLAMTDTIAAVPNKISVSGHTDAAPFVGSGGYGN
                     WELSADRANAARRVLIAGGYDDEQVARVVGYASSALFDRDNPLNPVNRRIDIVVLTKK
                     AQRAIEGEQGDAAVDDVPAEPGAPPVVPDAAAPTAPGDPLPDGQLRERFNIFEEGVLQ
                     FDQPAD"
     gene            complement(2135..3166)
                     /gene="rsgA"
                     /locus_tag="VFF22_12280"
     CDS             complement(2135..3166)
                     /gene="rsgA"
                     /locus_tag="VFF22_12280"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HZX17621.1"
                     /translation="MAKRQLNRRQNWRIEKIQGERAARAAKRESQAVQILEGGDLGPE
                     QTGLVIAHFGVQIEVEALDGELAGQVFRCHLRANLPTMVTGDQVVWRAGNQGIGVIVA
                     QLPRSTELCRPDPRGQLKPVAANVDLLVIVFAPLPEPHANLIDRYLVAAEHAGIRPLL
                     LLNKADLIDEQNGVALNAMLSVYRQLGYQLLEVSAHQGDGMEQLKQQLDGHISVFVGQ
                     SGVGKSSLVNSLLPDVDTRVGPLSEQSGLGTHTTTTARLFHFPGGGELIDSPGIREFG
                     LVHVSRDDVEAGFIEFSALIGQCRFRDCKHDREPGCALLKALEDGRLHPQRMASYRQI
                     LASLPEAEY"
     gene            3286..4059
                     /locus_tag="VFF22_12285"
     CDS             3286..4059
                     /locus_tag="VFF22_12285"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX17622.1"
                     /translation="MLRVVRQYPALLALSAQLGATLLIGLALYLTAQLSPWRPTWLAA
                     GLSQGMLAATLARYLGLSRWWCGLNLLFVPALLLASGAPLPSWVFLGGFVLLLLLNWN
                     SVGERVPLYLTAAGTRQHLCAVLDQQPASFRFIDLGCGPAGTLLGLARQFPQAQFVGV
                     ETAPLSFAIAWLRALPQGNCQIRYENLWRTDLAAFDVVYCFLSPAPMPELWAKACAQM
                     PAGSLLVSNSFEVPGQPPDQQIELHDWRKSRLLLWFMRG"
     gene            4100..4642
                     /gene="orn"
                     /locus_tag="VFF22_12290"
     CDS             4100..4642
                     /gene="orn"
                     /locus_tag="VFF22_12290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HZX17623.1"
                     /translation="MQNAQNLIWIDLEMTGLEPETDVIIEMATIVTDSDLNILAEGPV
                     IAVHQSDELLAAMDEWNTRTHGQSGLTQRVRESTIGPAEAEAMTIAFLEQWVPKGKSP
                     ICGNSIGQDRRFLYKYMQNLEAYFHYRYLDVSTLKILAGLWAPQVKESFQKQGTHQAL
                     DDIRESIAELKHYRQHFLKV"
     gene            complement(4688..5749)
                     /gene="queG"
                     /locus_tag="VFF22_12295"
     CDS             complement(4688..5749)
                     /gene="queG"
                     /locus_tag="VFF22_12295"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HZX17624.1"
                     /translation="MNTDTLDLAALAQSIKDWGRELGFQQVGIADVQLGEHEAHLQRW
                     LDAGYQGEMDYMAAHGSKRSHPEELVPGTLRVISLRMDYLPGDTQMAQRLAQPEKAYV
                     SRYALGRDYHKLIRKRLQQLAERIQHQIGPFGYRAFVDSAPVLEKAIAEQAGLGWIGK
                     NTLVLNRKAGSYFFLGELFVDLPLPVDEPHVTEHCGRCTACMDICPTAAFVGPYVLDA
                     RRCISYLTIELKGSIPEELRAPIGNRVFGCDDCQMVCPWNRFAKPTEQSDFQPRHSLD
                     NAELAELFCWSEEEFLSRTEGSPLRRAGYERWLRNLAVGLGNAPSSIPVLEALKARRE
                     HPSELVREHVEWALARHGL"
     gene            5810..7309
                     /locus_tag="VFF22_12300"
     CDS             5810..7309
                     /locus_tag="VFF22_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HZX17625.1"
                     /translation="MLHSPDNLPLPLYSAAQVRELDARLIAAGTPGFELMSRAAHAIW
                     RALRRRWPDAGVLTVLAGRGNNAGDGYLVAALALRAGWRVQVLAVGAAAQLQGDAALA
                     RDQALAAGVAVQPWSECAELQGVVLDALLGTGLQGAVREPYAQAIRLLNASDLPVLAV
                     DIPSGLCADTGQVLGVAVHAQLTVTLIGLKLGLFTGQSADYLGELQFDDLQADGALVA
                     AQAFTAQRLAQPRLPQLPVRSRSVHKGQLGHVLVVGGDRGFAGAGLLAAQSALRAGAG
                     LVSLATRGEHLPAAQARLPEVMAAGVASANQLLTLGANASVWVVGPGLGQGAWGRSLI
                     SAAAVSRAVQVWDADALNLLAQAVVKLPATAVLTPHPGEAARLLGVSTADVQADRPAA
                     ARALAQRYQAVCVLKGAGSLIAAADGGLWLCDRGHPVMAGAGLGDVLAGLIGALLAQG
                     LPALEAAQLAVYLHACAGERLALQGRGLAASDLCAVIRQLLQEQSACLN"
     gene            7297..7764
                     /gene="tsaE"
                     /locus_tag="VFF22_12305"
     CDS             7297..7764
                     /gene="tsaE"
                     /locus_tag="VFF22_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HZX17626.1"
                     /translation="MSELELFAADEEAMLALGARIAEATGGVGVIYLHGDLGAGKTTL
                     SRGIIRGLGHVGAVKSPTFTLVEPYEIGDLRAFHFDLYRLVDAEELEYLGIRDYFEGD
                     ALCLVEWPERGAGVLPKADLDITISPHGAGRTLRLQAQGERGTTWCAALAVGA"
     gene            7784..9202
                     /locus_tag="VFF22_12310"
     CDS             7784..9202
                     /locus_tag="VFF22_12310"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZX17627.1"
                     /translation="MRIGARFIAIGVALVGLAADVMAASDVRSVRLWRAPDNTRLVFD
                     LSGPVQHSVFLLSSPNRIVIDVSGAKLATSFNQLTLSNTPITGVRSAQRTPEDLRVVI
                     DLSAAVTPKSFTLAPNQQYGHRLVVDLFDQEGGAAPSLPTTTVATAPQVPVTPTQAPP
                     KLTPIPNGKRDIVIAIDAGHGGEDPGALSPVKGQYEKHVTLAISKELQRQINAEKGFR
                     AELVRTGDYFIPLRKRTEIARKKGADLFVSIHADAAPRAAAFGASVYALSDRGATSET
                     ARWLADAENQSDLIGGAGNVSLDDKDRMLAGVLLDLSMTASLSSSLNVGNKVLGNMGR
                     VTPLHKRRVEQAGFMVLKSPDIPSILVETGFISNPNEARKLSSSSHQQALARSIVTGV
                     RQFFHENPPPGTRVAWLRDNGKIALGPREHVVSSGESLALIAQRYQVSLAVLRSANSL
                     KSDVIKVGQRLQVPATALAGQP"
     gene            9199..11076
                     /gene="mutL"
                     /locus_tag="VFF22_12315"
     CDS             9199..11076
                     /gene="mutL"
                     /locus_tag="VFF22_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HZX17628.1"
                     /translation="MSEAARIQLLSPRLANQIAAGEVVERPASVIKELLENSLDSGAK
                     RIEVDVEQGGVKLLKVRDNGSGISPDDLPLALARHATSKIRELEDLEQVMSLGFRGEA
                     LASISSVARLTLTSRTADAEQAWQVETEGRDMQPRVQPAAHPVGTSVEVRDLFFNTPA
                     RRKFLKTEKTEFDHLQEVIKRLALARFDVAFHLRHNGKTVLGLHEAPDEQSRARRVAS
                     VCGPAFLEQALPIDIERNGLHLWGWVGLPTFSRSQADLQYFYVNGRMVRDKLVAHAVR
                     QAYRDVLFNGRHPTFVLFLEIDPSVVDVNVHPTKHEVRFRDGRMVHDFLYGTLHRALG
                     EVRPEDQLAAPATVNEMLRPTGQAAGEFGPQGEIGLAASLLQPTPSSSVWRSPGAGYQ
                     GSRPDAVLPAAEAQGAYREFFSPMATAPAPAGLPDAQGDIPPLGYAIAQLKGIYILAE
                     NVQGLVVVDMHAAHERITYERLKVAMASEGLRGQPLLVPESIAVSQREADCAEEHAEW
                     FQRLGFELQRLGPETLAIRQIPALLKQAEATRLVQDVLGDLLEYGTTDRIQAHLNELL
                     GTMACHGAVRANRRLTLPEMNALLRDMEHTERSGQCNHGRPTWTQMGMDDLDKLFLRG
                     R"
     gene            11105..12082
                     /gene="miaA"
                     /locus_tag="VFF22_12320"
     CDS             11105..12082
                     /gene="miaA"
                     /locus_tag="VFF22_12320"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZX17629.1"
                     /translation="MSERLPPAIFLMGPTAAGKTDLAIELARVLPCELISVDSALIYR
                     DMDIGTAKPDKATLAEFPHRLIDIRDPAESYSAAEFRADALAAMAEITARGRIPLLVG
                     GTMLYFKALLEGLAEMPSADVALRAELEARAQAEGWAVLHAELRAVDPESAARIHPND
                     PQRLIRALEVYHLSGHSMTVHRLRQAAENTAGDASAARQFPYTVAQLAIAPAQRQLLH
                     ERIALRFRLMLEQGLVAEVERLRQRSDLHVGLPSIRAVGYRQVWDYLDGNLDAEQMQE
                     RGIIATRQLAKRQFTWLRGWQQDVHWLDSADCDNLPRALKYLAAVSILT"
     gene            12185..>12407
                     /gene="hfq"
                     /locus_tag="VFF22_12325"
     CDS             12185..>12407
                     /gene="hfq"
                     /locus_tag="VFF22_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HZX17630.1"
                     /translation="MSKGHSLQDPYLNTLRKERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTAVPSRPVRSPSVS"
BASE COUNT         2185 a         3658 c         4006 g         2558 t
ORIGIN      
        1 tttggcatgc gggttgagac aagctggctt ttgaccttaa ctggctatag tctcgcgcaa
       61 aacttccgat aaatagaagg gttgttttgc aaagcccctt tctaccctct ctggcaagtc
      121 tctttctatg gcaaaaatca ttggcatcat tgttgtactc gccagcgtgt tgggcggttt
      181 tatcctttcc ggtggccagt ttatggcgtt ggtccacccc ttcgagattc tgattatcgg
      241 tggtgctgca ctcggggctt tcctccaggc caaccctggc agcgccttta tgcatgtgtt
      301 caagaagtcg ctgagcatgt tcagctcgcg ctttacccat gcctattacc tcgatgtgct
      361 caagctgctt tacgagattc tcaacaagag ccgtcgcgaa ggcatgatgg ccattgaggc
      421 cgacgtcgaa gatccggccg ccagcccgat tttcagtaag taccccggca tcctcaagga
      481 tcagcagatg accgccttta tctgcgatta cctgcgcatc atgtcttctg gcaacatggc
      541 tccgcatgag ctggaaggcc tgttcgatat ggagatatcc agcctcaaag aagaagttga
      601 gcacccggcg catgccgtag ccaaggtggc cgacggtatg ccggccatgg gtatcgtggc
      661 agcggtattg ggcattgtga tcaccatgtc gattcttgcc gaagcggata acgcggagat
      721 cggtcagaag gtcggtactg ccttggtggg taccttcttc gggattctgg cgtcctatgg
      781 ctttttcggc cctttgtcgg ccgccttgga gcacgacgcc aaggaagagc tgaacgtcta
      841 tgaggcgatc aaagccggcc tggtggcttc ggcctccggc atgccgccat cgctggctgt
      901 agagttttcc cgcaaggtgc tgttgcctgc gcatcgccca agctttgccg agcttgagca
      961 ggccatgcga gggggctaag gcacagcatg gaaaataatc agccgattat cgtcaaacgg
     1021 gtcaaaaagg ttgccggcgg ccatcatggt ggtgcctgga aaattgcctt tgccgacttt
     1081 gccacggcga tgatggcgtt ctttctagtg atgtggctga tgacggtggc cacacccgag
     1141 cagaagaagc tgatttccgg ttactttcag gaccctgtcg ggttctcgga aagtgccagc
     1201 ccctatgtga ttgacttggg cggcacgcct acgccatcgc cggaccgcac cctcaatgat
     1261 cagccgcagg aagagcagag cccggccgag gatgcgcagg ccgatgtgga caagctgcag
     1321 gccgagtcga tagccgaaga agtcgagcgt gagcgcctgg aattgttgct gcaggaattg
     1381 cagaacaagg ttgaagaaaa ccctcagctg cagaatttca aggatcagat cctcttcgaa
     1441 atcacccagg acggcttgcg catccagatc atggatgccg agaaccggcc gatgtttgct
     1501 tccggcagcg cgcgcctgca gccctatttc gaagacattc tgctggccat gaccgacacc
     1561 atcgccgcag tgccgaataa gatcagtgtc agtggccata ccgatgcggc accctttgtc
     1621 gggagtggtg gttatggcaa ctgggagctg tctgctgacc gagccaatgc ggcgcggcgc
     1681 gtactgattg ccggcggcta tgacgatgaa caggtcgcgc gagttgtggg ttacgcgtcc
     1741 tcggccttgt ttgatcggga caatccgctc aatccggtca accggcgtat cgatatagtg
     1801 gtgctgacca agaaagccca gcgtgccatc gaaggcgaac agggtgacgc cgctgtagat
     1861 gatgtgcctg cagagcctgg tgcgcccccc gttgtgccgg atgcagcggc gcccaccgcg
     1921 ccaggtgatc cattacctga tgggcagttg cgcgagcgtt tcaatatctt tgaggaaggc
     1981 gtgttgcagt tcgatcagcc cgccgattga gctgcagctg caagtaaaaa ggccgcgata
     2041 tgtcgcggcc ttttttgttg ctggcctgcc gtccctggcg gccaccctgc aggccgtcgc
     2101 ggcgcgacgt taaaaatcac tcccggcgat tttttcagta ctcggcttct ggcaggctgg
     2161 ccaggatttg ccggtaactg gccatgcgct gcgggtgcag acggccgtct tccagggctt
     2221 tgagcagggc gcagcccggc tcgcgatcgt gtttgcagtc gcgaaagcgg cattgcccga
     2281 tcagcgcgct aaactcgata aagccagctt cgacgtcatc acggctgaca tgtaccaagc
     2341 cgaattcgcg aatccctggg gagtcaatca gctcgccgcc gccaggaaag tgaaacagcc
     2401 gtgcggtcgt cgtggtgtga gtgcccaggc cggactgctc cgaaagcggc ccaacgcggg
     2461 tgtcgacatc gggtagcagg ctgtttacca gcgaggattt acccacgccg gattgcccta
     2521 caaacacact gatatgcccg tcaagctgct gcttgagctg ctccatgcca tcaccctgat
     2581 gcgcagagac ctcgagcagc tgataaccca gctggcggta aacgctgagc atggcgttga
     2641 gtgccacgcc gttttgttca tcaatcaggt cggctttatt caacagtaat agggggcgga
     2701 taccggcgtg ctcggcagcc accaggtaac ggtcgatcag gttggcgtgg ggctcaggca
     2761 gcggcgcgaa aacaatcacg agcaaatcga cattggccgc taccggcttc agctggccgc
     2821 gagggtccgg gcgacagagt tcggtgctgc gtggcagttg cgcaacgatt accccgatgc
     2881 cctgattgcc agcgcgccac accacttggt caccggtgac catggttggc aggttggcgc
     2941 gcagatggca gcggaatacc tggccggcca gttcgccatc cagtgcttca acttcgatct
     3001 gtacaccgaa gtgggcgatc accaggccgg tctgttccgg gccgaggtcg ccgccttcaa
     3061 gaatctgtac agcctgcgac tcgcgcttgg cagcgcgggc ggcacgctcg ccctggattt
     3121 tttcgatacg ccagttttgc cggcggttga gttggcgttt ggccatgggt cttccgagag
     3181 ttgaattacg caataaacgg ccgcgagtct agcacgggct gcctattgag ctgctggcgc
     3241 ggctaaactg cggcgcatca ttgatcggac gattcaggag tctgcatgct gcgcgttgtc
     3301 cgccaatatc cggctctgtt ggccttgtca gctcagctcg gtgcgacctt gctgatcggt
     3361 ttggcgctgt atctgacggc ccagttgtcg ccctggcggc cgacctggct agccgcaggc
     3421 ctgtcgcaag gcatgttggc tgccacgctg gcacgctacc tggggctgtc gcgctggtgg
     3481 tgcggattga acctgctgtt cgtcccggcg ctgctgctgg ccagtggtgc gccgctgccg
     3541 tcctgggtgt ttcttggcgg ctttgtcctg ttgctgctgc tcaactggaa cagtgttggc
     3601 gagcgggtgc cgctgtacct gaccgctgcc ggtacgcgcc agcatctgtg tgcggtgctt
     3661 gaccagcagc ctgcatcgtt tcgctttatc gacctgggtt gtggtccggc cggtacgttg
     3721 ctgggcctgg cccggcagtt cccgcaggcg cagtttgtcg gggtggagac tgcgccgctg
     3781 tcctttgcta tcgcctggtt gcgtgctttg ccgcagggca attgccagat ccgttacgaa
     3841 aacctctggc gcacggacct ggctgcgttt gatgtggtgt attgctttct gtcccctgcg
     3901 cccatgcccg agctgtgggc caaagcctgc gcgcagatgc cggccggcag tctgctggtc
     3961 agcaacagct tcgaggtgcc ggggcagccg cctgatcagc aaatcgagct gcacgactgg
     4021 cgcaagtcac ggctgctgct gtggttcatg cgcggctgat ttattggcta aactgtcggc
     4081 cttagtcgag gagcgcagca tgcagaacgc acagaaccta atctggatcg atctggaaat
     4141 gaccgggctg gagcccgaaa cggatgtcat catcgagatg gccaccattg tcaccgacag
     4201 cgacctgaac atcctcgccg aaggcccggt gattgccgtg catcagagtg atgaactgct
     4261 cgcggcaatg gatgagtgga acacccgcac ccacggccag agcgggctga cccagcgcgt
     4321 acgggaaagc accatcggcc cggcggaagc ggaggccatg accattgcct tccttgagca
     4381 gtgggtgccg aagggcaagt cgccgatctg cggcaacagt atcggccagg atcggcgctt
     4441 tctttataag tacatgcaga acctggaagc ctacttccac taccgctatc tggatgtctc
     4501 gaccctgaag attctcgccg gcctgtgggc gccgcaggtc aaggaatcgt tccagaagca
     4561 gggcacgcac caggcactgg acgatatccg cgagtcgatt gccgaactga agcactaccg
     4621 tcagcacttc cttaaagtct gacgcagtga aaagcccgct agtgcgggct tttttatgtt
     4681 tgctcggtta caggccgtgg cgcgccagag cccattcaac atgctcgcgc accagctcgg
     4741 acgggtgctc gcggcgcgcc ttgagcgctt ccagcaccgg gatgctggag ggcgcgttgc
     4801 ccaagccgac ggccaggttg cgcagccagc gctcgtagcc ggcgcggcgc aggggtgagc
     4861 cttcggtgcg gctgagaaat tcctcttcgc tccaacagaa cagttcggcc agctctgcgt
     4921 tgtccaggct gtggcgcggc tggaagtcgc tctgctcggt gggtttggca aagcggttcc
     4981 acgggcagac catctggcag tcgtcgcagc cgaacacccg gttgccgatc ggtgcacgca
     5041 attcctccgg aatcgagccc ttgagttcga tggtcaggta ggaaatgcag cgccgcgcat
     5101 ccagcacata ggggccgaca aaggcggcgg tggggcagat atccatgcag gcggtgcagc
     5161 gcccgcagtg ctccgtgaca tggggttcat ccaccggcag tggcagatca acaaacagct
     5221 cgccgagaaa gaagtagctg ccggccttgc ggttaagcac cagggtgttc ttgccgatcc
     5281 agccgagccc agcctgttcg gcgatggcct tttccagcac tggcgcgctg tcgacaaagg
     5341 cgcggtagcc gaacgggccg atctgatgct ggatgcgttc ggctaattgt tgcaggcgtt
     5401 tgcggatcag cttgtggtaa tcgcggccta gggcgtagcg cgatacgtag gccttctccg
     5461 gctgggcgag gcgctgggcc atctgcgtgt cgccgggcag gtagtccata cgcagtgaga
     5521 tgactctcaa ggttccgggc accaattctt ccgggtggga acgtttgctg ccgtgggcgg
     5581 ccatatagtc catctcgccc tggtaaccag cgtccagcca gcgttgcagg tgcgcctcat
     5641 gttcgcccag ttgcacatcg gcaatcccga cctgttgaaa gcccagctcg cggccccagt
     5701 ctttgatcga ctgggcgagg gcggcaaggt caagcgtgtc agtgttcata tgagggcagg
     5761 taaccggggc gcaggtgggt ataattctgc cagacatcgg agccgacgca tgctgcattc
     5821 gcctgacaat cttcctctgc cgctatacag cgccgcccaa gtgcgcgagc tggatgcgcg
     5881 attgattgct gccggcacgc cgggtttcga gctgatgagc cgcgccgcac atgccatctg
     5941 gcgtgccctg cgtaggcgtt ggccggatgc gggtgtgctg acggtgctgg ctgggcgcgg
     6001 taacaacgcc ggtgacggct acctggtggc cgctttggcg ttgcgcgcgg gctggcgggt
     6061 gcaggtgctg gcggtcggcg ctgcggcgca gttgcagggc gatgctgcct tggcccgtga
     6121 ccaggcgctg gctgccggtg tagcggtgca gccctggagc gaatgcgccg agctgcaagg
     6181 cgtggtgctc gatgccttgc tcggcacggg cttgcagggc gcagtgcgcg aaccgtatgc
     6241 ccaggccatc cggctgttga atgccagtga tctgccggtg ctggcggtgg atattccgtc
     6301 cgggctctgc gccgatactg gccaggtgct cggcgttgca gtgcatgcgc aactgacggt
     6361 caccctgatc ggcctgaagt tgggcctgtt tactgggcag tcagcggatt atctgggtga
     6421 gttgcagttt gatgatctgc aggctgatgg ggccctggtc gcagctcaag cctttaccgc
     6481 gcagcgcctg gcgcagcccc gcctgccgca attgccggtg cgttcgcgta gcgtgcataa
     6541 gggccagttg ggccatgtgc tggtggtcgg cggtgatcgc ggctttgccg gggctggcct
     6601 gctggcggcg caaagtgcgc tacgcgccgg tgccgggctg gtcagcctgg cgacccgtgg
     6661 cgagcatttg cctgcagcgc aggcgcgcct gcccgaggta atggccgctg gcgtggcctc
     6721 ggccaatcag cttctgacgc tgggcgccaa tgcaagcgtc tgggtggtcg gcccaggctt
     6781 ggggcagggc gcctggggtc gcagtctgat atcggcggct gcggttagcc gtgcggtaca
     6841 ggtttgggat gccgatgcgc tgaacctgtt ggcgcaagct gtagtcaagt tgcctgctac
     6901 agcggtgctc accccacacc ctggcgaagc ggcgcggctg ctgggtgtca gcacggccga
     6961 tgtgcaggcc gatcgtccgg cggcggcccg cgccttggcc cagcgctatc aagctgtatg
     7021 cgtgctgaaa ggcgccggca gcctgattgc tgcggcggat ggcggcttgt ggctgtgtga
     7081 tcgcggccat ccagtcatgg ccggcgctgg gctcggggat gtgcttgccg ggcttatcgg
     7141 cgcattgttg gcccagggcc tgcctgcgct ggaggcggct cagctggcgg tctatctgca
     7201 tgcctgcgcg ggcgagcggc tggcgctgca ggggcgcggc ctggcggcca gtgatttgtg
     7261 tgcggttatt cgtcaattgt tgcaggagca gtctgcgtgt ctgaattaga gctgtttgcc
     7321 gccgatgaag aggcgatgct ggcgctgggg gcacgtatcg ccgaggcgac cggcggcgtt
     7381 ggcgtgatct atctgcatgg cgacctcggt gccggcaaaa ccaccctatc gcgcggcatt
     7441 attcgcggcc tgggccatgt tggggcggtg aaaagcccga cctttaccct ggtcgaaccc
     7501 tatgaaatag gcgacctgcg cgcctttcac ttcgaccttt accgtctggt ggatgccgag
     7561 gagctggagt acctggggat tcgtgattac tttgaaggcg atgcgctgtg tctggttgag
     7621 tggccggagc gcggtgcagg cgttttgcca aaggctgacc tggacattac cattagcccg
     7681 catggggctg gccggacgct gcgtttgcag gcgcaaggcg aacgcggcac cacctggtgc
     7741 gccgctttgg ccgtcggggc atgaatcaat tatggggttg gctatgcgca taggcgcgcg
     7801 tttcatcgca attggagtgg cattggtggg cttggccgcc gatgtaatgg ccgcttcgga
     7861 tgtgcgcagt gtgcggctgt ggcgtgcgcc ggataacacc cgcctggtgt ttgacctgtc
     7921 cggcccggtg cagcacagcg tttttctgct gtcttcgccc aatcgcatcg tcattgatgt
     7981 cagcggtgcc aagctagcca ccagctttaa tcagctgacc ctgagtaaca ctccgatcac
     8041 cggcgtacgc tcggcgcagc gtacccccga agacttgcgc gtggtgattg acctgtcggc
     8101 agcggtgacg ccgaagagct ttaccctcgc ccccaatcag cagtacggcc atcgcctggt
     8161 ggtcgatctg tttgaccagg aaggcggcgc cgcacccagc ctgccaacca ccaccgtggc
     8221 cactgcgccg caggtgccgg taacgccaac ccaggccccg ccaaagctga cgccgatacc
     8281 caacggcaag cgcgatatcg tgattgccat tgatgcgggc catggcggcg aagacccagg
     8341 cgcgttgtcg ccggtcaaag gccagtatga aaagcacgtg accctggcga tttccaagga
     8401 gctgcagcgg cagatcaatg ccgagaaggg cttccgcgcc gagctggtgc gtaccggcga
     8461 ttactttatc ccgctgcgca agcgcaccga gatcgcccgc aagaagggcg ccgacctgtt
     8521 tgtttccatc cacgccgatg ctgcaccgcg ggcggcggca tttggtgcct cggtgtatgc
     8581 gctgtcggat cgcggcgcca cctcggaaac cgcgcgctgg ttggcggatg cggaaaacca
     8641 gtcggacctg atcggcggcg ccggcaatgt cagcctggac gacaaggacc gcatgctggc
     8701 cggggtgttg ctcgacctgt cgatgactgc ctcgctgtca tccagcctga acgtcggcaa
     8761 caaggtgctg ggcaatatgg gccgagtgac tccgttgcat aaacggcggg tggaacaggc
     8821 cggctttatg gtgctgaagt cgccggatat tccgtcgatc cttgtggaaa ccgggtttat
     8881 ctccaacccc aatgaagccc gcaaactgtc cagttccagt caccagcagg ccctggcgcg
     8941 ctcaatcgtc acgggcgtgc ggcagttttt ccatgaaaac ccgccgcccg gcactcgcgt
     9001 ggcctggctg cgcgacaacg gcaagattgc cctcggcccg cgcgagcatg tggtcagctc
     9061 cggcgaaagc ctggcgctga ttgcacagcg ttatcaggtc agcctggccg tgctgcgcag
     9121 tgccaactcg ctgaaaagcg atgtgatcaa ggtcggtcag cgtttgcagg tccctgcgac
     9181 tgctctggct gggcagccat gagtgaagct gcacgtatcc agctgctcag cccgcggctg
     9241 gcgaaccaga ttgctgccgg tgaggtggtt gaacgcccgg cctcggtaat caaggagctg
     9301 ttggaaaaca gcctggactc cggcgccaag cgcatcgaag tggatgtcga gcagggcggc
     9361 gtcaagctgc tcaaagtgcg tgacaacggt agcggcatct ccccggatga cctgcccctg
     9421 gcgctggccc gccatgccac cagcaagatc cgcgagctgg aagacctgga gcaggtgatg
     9481 agtctgggct ttcgcggtga ggcgttggct tctatcagct ccgtcgcacg tctgaccctg
     9541 acctcgcgta ccgctgatgc tgaacaggcc tggcaggttg aaacagaagg gcgcgatatg
     9601 cagccacggg tacagcccgc tgcgcacccg gtcggcacct cggtggaagt gcgcgacctg
     9661 ttcttcaaca cccctgcacg gcgtaagttc ctcaaaaccg aaaaaaccga attcgatcat
     9721 ctgcaggaag tgatcaagcg cctggccctg gcgcgcttcg atgtggcctt ccatctgcgc
     9781 cacaacggca agaccgtgct cggcctgcat gaggctccag atgaacagag ccgcgcgcgg
     9841 cgggtggctt cggtctgtgg cccggcgttt ctcgagcagg cgttgcccat cgatatcgaa
     9901 cgcaacggcc tgcacctgtg gggttgggtt ggtttgccga ccttctcccg tagccaggcg
     9961 gatctgcagt acttctatgt gaatgggcgc atggtgcgcg acaagctggt tgcccatgcg
    10021 gtgcgccagg cctaccgcga cgtgctgttc aatggtcggc atccgacctt tgtgctgttt
    10081 ctggaaattg acccctcggt agtcgatgtc aacgtgcacc cgaccaagca tgaagtgcgc
    10141 ttccgtgacg ggcgcatggt gcatgacttc ctctacggca cgctgcatcg cgcattaggc
    10201 gaggtgcgcc cggaagatca gctggcggcc ccggcgacgg tcaacgagat gctgcgccct
    10261 accggccagg ctgccggtga gtttggtccg cagggtgaaa tcggcttagc tgccagcctg
    10321 ctgcagccta cgcccagcag ctcggtgtgg cgttcgccgg gcgccggcta tcagggctct
    10381 cgtcctgacg cagtcttgcc ggccgccgaa gcccaaggcg cctatcgtga attcttctcg
    10441 ccgatggcca ctgcgccggc tcccgctggc ctgccagatg cgcagggcga tattccgccc
    10501 ctgggctatg ccatcgccca gctcaagggc atctacatcc tcgccgaaaa tgtccagggt
    10561 ctggtggtgg tggatatgca cgccgcccat gagcgcatca cctatgagcg gctcaaggtg
    10621 gccatggcca gcgaaggcct gcgcggccag ccgctgttgg tgccggagtc tattgccgtc
    10681 agccagcgtg aagctgactg cgccgaagag catgccgagt ggttccagcg acttggcttt
    10741 gagttacagc gtttgggtcc ggaaaccctg gctatccggc agattcccgc attgctcaag
    10801 caggccgagg ccactcgtct ggtgcaggat gtgctcggtg atctgctcga atacggcact
    10861 accgaccgca ttcaggcgca cctcaacgag ctgctcggca ccatggcttg ccacggtgcc
    10921 gtgcgcgcca accgacgcct gaccctgccg gaaatgaacg ccttgctgcg cgatatggaa
    10981 cacaccgagc gcagcggcca gtgcaaccat gggcggccga cctggacgca gatgggcatg
    11041 gatgacctcg acaagctgtt cctgcgcggc cgctagccat cgcgccattt agagaatttt
    11101 gaacatgtcc gagcgcctgc ctccggcaat ttttcttatg gggccaacag cggccggcaa
    11161 gaccgatctg gccatcgagc tggcgcgagt gctgccgtgc gagctgatca gcgtcgactc
    11221 tgcgttgatc taccgtgata tggacatcgg cacggccaag ccggataagg ccactctggc
    11281 agagtttcct caccgcctga tcgatatccg tgacccagcc gagagctatt cagcggccga
    11341 gttccgtgcc gatgccctgg cggctatggc tgagatcaca gcgcgcggac ggattccgct
    11401 gctggtgggc ggcactatgt tgtatttcaa ggcgttgctt gaggggctgg ccgaaatgcc
    11461 cagcgccgat gtggcgttgc gcgccgagtt ggaggcgcgc gcccaggccg aaggttgggc
    11521 ggtgctgcat gccgagctgc gtgcggtcga tccggagtcg gctgcgcgta tccatcctaa
    11581 tgacccgcag cgcttgattc gcgcccttga ggtctatcac ctgagcgggc acagcatgac
    11641 cgttcatcgt ctgcgccagg cggcagaaaa tactgctggt gacgcatcgg ctgcgcggca
    11701 attcccctat actgtggcgc aattggccat tgccccggcg caaaggcagc tgttgcacga
    11761 gcgaatcgcc ctgcgatttc gcctgatgtt ggaacagggg ctggttgccg aggtcgaacg
    11821 cctgcgccag cgaagtgact tgcacgttgg tctaccgtct atacgagcgg tgggttatcg
    11881 gcaagtctgg gactatctgg atggcaacct cgacgccgag cagatgcagg agcgcggcat
    11941 tatcgccacg cgccagttgg ccaagcgaca gttcacctgg ttacgtggtt ggcagcagga
    12001 tgtgcactgg ctcgatagcg ccgactgcga caatctgccg cgtgccttga aatacctggc
    12061 agcggtctcc atattgacct gagaccttgc cgttggccgt ctactcttgg gggtacgacg
    12121 gctttaagcc gttcgttggt ttactaaaaa ttattgaaaa ttgatcctta aaggagtgcg
    12181 gcacatgtca aaagggcatt cgctacaaga cccttacctg aataccctgc gtaaggaacg
    12241 cgtcccggtt tccatctatc tggtcaacgg catcaagctg caaggtcaga tcgagtcgtt
    12301 cgaccagttc gtgattttgc tgaaaaatac tgtcagccag atggtctaca agcacgctat
    12361 ctccactgcg gtgccaagcc gcccggtgcg ttcgccgagc gtttccg
//
LOCUS       DASRUM010000190         7333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1926920, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000190 DASRUM010000000
VERSION     DASRUM010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926920"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1824)
                     /locus_tag="VFF22_12330"
     CDS             complement(199..1824)
                     /locus_tag="VFF22_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015096529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX17631.1"
                     /translation="MFLQKSLRAQILALLGGSLALILVTALACFSFLSSGMQAYRGLL
                     EGPLEASALIESTNLNFKTQVQEWKNVLLRGSDKAQLDKYWSQFEAQERKVQDSLGTL
                     SELAGADRTLKTKVDALRSEHQTLGVNYRKGRDAFVAAGGDAAVGDKAVSGIDRNTAQ
                     QLEALSSELHKQSLEQASLINASADRTITYGTLLMLGATVVIALFSLWLINRNLINPI
                     RELIEHIAQLSQGNFGQHVESTRQDELGNLAIAANILRDFLSDTFTRLKRSTADLDTA
                     SGELNSIATLMAQGTREQFSRTDQVATAMHEMSATAQEVARHAAEAAGAADAADSAAR
                     QGETVMQATIKTITDIRSEISNTSDVIRRLESDSGRIGKVLEVIRGIAEQTNLLALNA
                     AIEAARAGEQGRGFAVVADEVRTLAQRTAESTAEIHQIIDTVQTGAVNAVRAIESGQH
                     RSEEGVTQVTEAGATLQLITSAVEAIRDMNRQIATAAEEQTSVAEDISRNLTEITAIA
                     TANQENVQRTESAGQNLQVLSGQLNEVTQRLSA"
     gene            complement(2014..3123)
                     /locus_tag="VFF22_12335"
     CDS             complement(2014..3123)
                     /locus_tag="VFF22_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1513 domain-containing protein"
                     /protein_id="HZX17632.1"
                     /translation="MNRRAFLGLSIAAVAATAAAAGTYGGWTLMHSSAQPLLLSARND
                     SAGKHFAVGYRLDGSQVFATQVSERCHDVIGHPFLPMALFVGRRPSTQSYLIDTRDGR
                     LLQTLNSPKQRHFYGHAVFHKDGEWLYATENDTSDPGRGVLGVYRLQGEQLLRSSELS
                     THGIGPHQLLWLPDGETLAVANGGIRTEADSRVEMNLDAMESSLVLLRRDGSLISKEQ
                     LPERMNSVRHMAVASDGTLVSGQQYMGELSDAVPLLAIKRPGQPFQHFPVADAQRQLM
                     NQYTASVAIHSELRLLALTAPRGNRVFIWDLDSAELRLDAPLADCAGVGAVADGFVVS
                     SGVGRCRLYNCRSAQITSQPLQLPAGLWDNHLRLA"
     gene            complement(3133..4194)
                     /locus_tag="VFF22_12340"
     CDS             complement(3133..4194)
                     /locus_tag="VFF22_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HZX17633.1"
                     /translation="MIRSPLTLALLSLTLTACSPSNPQQQVSRALTEGVLLPIYSAWS
                     EADRQLAVSGQAFCSGQQSLTDARLAFSSAQSAWAALQPVAFGPLAEGNRAWQVQFWP
                     DKKNLVARQVEALINSKPELTPADLDKSSVVVQGLTAYEYLLFDPSIDLNVAEQKTRY
                     CPLIVAIGQHQQVLAADVLSQWQAKDGMAALLKDFPNARYAEAPEAVAELLRTQVSAI
                     DGLKKKLGTPLGRQSKGQPQPYQAEAWRSKASLANLAASLASAERIWLGAEQDGIQAL
                     LGSDQDELKQRINAAYSDTRQRLAAAQRPLGELLSDEAGRTEVNALYDSLNVLHRLHE
                     SELAKTLGIQLGFNAHDGD"
     gene            complement(4205..5620)
                     /locus_tag="VFF22_12345"
     CDS             complement(4205..5620)
                     /locus_tag="VFF22_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HZX17634.1"
                     /translation="MFAPHRIALLLALGLSLVACDKTPQFTQAEPGEALSAGSATVRK
                     ADQNAFSLPSANLSPSRRLDFSVGNSFFRNPWVTSPATTTARDGLGPLFNTNACQNCH
                     IKDGRGHPPAPDAQSAASMLVRLSIPASSEHSEIIQRLGVLAEPTYGAQLQDMANPGV
                     TPEGKVRVSYSTQLVSFADGQQVELRKPSLEISQLGYGDLHPDTLFSARIAPPMIGLG
                     LLEAIPEAAILANADPDDGNGDGISGRANQVWDREQQRTVLGRFGWKAGQPTLNQQNA
                     EAFANDTGLTSTLVTHDNCTTAQTDCRSAPHGGEPEVSDNILASVLFYTRNLGVPARR
                     GADTPQVLAGKNLFYQAGCQGCHSPQFTTASDAAEPELANQVIRPYSDLLLHDMGADL
                     ADQRPEFLASGSEWRTPPLWGIGLTETVNGHTQFLHDGRARNLLEAILWHGGEAEAAK
                     QQVLTFNADERSALLAFLNSL"
     gene            complement(5893..7227)
                     /locus_tag="VFF22_12350"
     CDS             complement(5893..7227)
                     /locus_tag="VFF22_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009050527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HZX17635.1"
                     /translation="MLRMPLASASLLAIAISLAGCGEDKAPATQAAAPAASSASAPAA
                     ATSQVDEAASKAVVSHYADLALAVFSDAASTGKTLQGAIDALLANPNDDTLKAAREAW
                     LAARVPYMQTEVFRFGNAVVDDWEGQLNAWPLDEGLIDYVAKDYQHALGNPGASANII
                     ANTELQVGEDKLDVTTITPELLANLNELGGSEANVATGYHAIEFLLWGQDLNGTNPGA
                     GERPASDFVVGEGATGGNNERRRAFIKAAADLLVSDLDAMVVQWQAGKTDNYRATLEA
                     DTAENGLRKMLFGMGSLSLGELAGERMKVALEANSTEDEHDCFSDNTHNSHFYNGKGI
                     RNVYLGEYKKVDGTTLTGPSLSSLVAKVDAAADSTLKADLEATEGKLQALVDSANNGK
                     HFDQLIAADNAEGQQIVRDAIAALVKQTGAIEQAAGKLGISDLNPDTADHSF"
BASE COUNT         1234 a         2075 c         2469 g         1555 t
ORIGIN      
        1 agcaacatat tccggttcct tggcaaggcg gttggatagc ctgggaatag gcaagagggg
       61 cacattagac ctcgcgcaca ggcctgtcaa agattcgcat ttgcaaaata ttgttgtgca
      121 aaagttgcct gattgatcaa gtacttttta agcaaaatgc cagcatatgc tggcattttg
      181 tgtggtgcgt tgcgctgatc aggcactcag gcgctgggtc acctcgttga gttggccgga
      241 caggacttgc aggttttgcc cggcgctttc ggtgcgttgt acgttctcct ggttggccgt
      301 ggcgatggcg gtgatttcgg taaggttgcg tgagatgtct tcggctaccg aggtttgctc
      361 ttcggcggcg gtggcgatct gccggttcat atcgcgaatt gcttccactg cactggtgat
      421 cagttgcagc gtcgctccgg cctcggtgac ctgagtcacg ccttcctcgc tgcggtgctg
      481 gccgctttcg atggcgcgca ctgcatttac tgcaccggtt tgcacggtgt cgatgatttg
      541 gtggatttcc gccgtggact cggcggtgcg ttgcgccagg gtgcgtactt catccgctac
      601 cacggcaaaa ccgcggccct gttcgcccgc gcgggcggct tcgatggcgg cgttgagcgc
      661 cagcaggttg gtctgctcgg cgataccgcg aatcacttcc aacaccttgc cgatacgccc
      721 gctgtcgctt tccaggcggc gaatcacatc cgaggtgttg ctgatttcgc tgcggatatc
      781 ggtgatggtc ttgatggtcg cctgcatcac cgtttcgccc tggcgtgcag cgctgtcagc
      841 ggcatcggcg gcgccggcgg cttctgcagc gtgacgcgcg acttcctggg cggtggcgga
      901 catttcatgc atggccgtgg cgacctgatc ggtacgtgag aactgctcgc gggtgccctg
      961 ggccatcagc gtggcgatgg agttgagttc gccgctggcg gtgtccagat cagccgtgct
     1021 gcgtttcaga cgggtgaagg tgtcggagag aaaatcgcgc aggatattcg cggcaatggc
     1081 caggttgccc agttcatcct ggcgagtcga ctccacatgc tggccgaagt tgccctggct
     1141 aagctgggcg atatgctcaa tcaactcgcg aatcgggttg atcaggttgc ggttgattag
     1201 ccacaggctg aacagtgcaa tgaccacggt ggcgccaagc atcagcaggg tgccgtaggt
     1261 gatggtgcgg tcggccgagg cgttgatcag gctggcttgc tccaggctct gcttgtgcag
     1321 ctcgctggac agcgcttcca attgttgggc ggtattgcgg tcaataccgg acaccgcctt
     1381 gtcgccgaca gctgcgtcac caccggccgc gacaaaggca tcgcgaccct tgcggtagtt
     1441 gacccctagc gtctggtgtt cgctgcgcag tgcgtcgact ttggttttca gcgtgcggtc
     1501 ggcgccagcc agctcactca acgtgcccag agagtcttga accttgcgtt cctgcgcttc
     1561 gaactggctc cagtatttgt ccagctgggc tttgtcgctg ccacgcagca ggacgttttt
     1621 ccattcctgt acttgggtct tgaagttcag gttggtcgat tcgatcagtg ctgaggcttc
     1681 taacgggccc tcaagtagac cgcggtaagc ttgcatgccg ctggaaagga agctgaagca
     1741 agccagtgcg gtgaccagaa tcagcgccag gctgccgccg agcagggcga gaatttgggc
     1801 gcgcaaagat ttttgcagaa acatggacga tacccctgaa tgaagaaggc gggatgccag
     1861 tgacgcagaa ggcgtccgaa ctgcgaagat ccctatcgac tggtatcggc aagccgagtg
     1921 cctggaagca gcggctgctg gaagtataga tcggctttgc ggcagcgttc tttagcgtgc
     1981 agcaggtgtc ttccctgttc cgtaacgggg catttaggcc aggcgcaggt ggttgtccca
     2041 caggccggca ggcagttgta gcggctggct ggtgatctgg gcgctacggc agttgtacag
     2101 gcggcaacgg ccaacgccac tgctgaccac aaagccgtcg gctaccgcgc caacgccggc
     2161 gcagtcagcg aggggggcgt ccaggcgcag ttcggcgctg tccaggtccc agataaacac
     2221 ccggttgccg cgcggcgcgg tcaacgcgag caggcgcagc tcgctgtgaa tcgccacgct
     2281 ggcggtgtac tggttcatca gctggcgttg cgcgtccgcc accgggaagt gctggaaggg
     2341 ctggccaggg cgcttgatcg ccagcagcgg caccgcatcg ctgagctcgc ccatatattg
     2401 ctgcccgctg accagggtgc cgtcgctggc cacggccata tggcgcacgc tgttcatccg
     2461 ctctggcaac tgctctttgc tgatcaggct gccgtcgcga cgcagcagca ccaggctgga
     2521 ttccatggcg tcgaggttca tttctacgcg gctatcggcc tcggtgcgga tgccgccatt
     2581 ggccacggcc agggtttcgc cgtctggtag ccagagcaac tggtgcggac cgatgccgtg
     2641 ggtcgacagt tcgctgctgc gcagcaactg ttcaccttgc aggcgataga cgccgagcac
     2701 gccgcggcca gggtcggagg tgtcgttctc ggtggcgtac agccattcgc cgtccttgtg
     2761 gaacaccgca tggccataga aatggcgctg ttttggcgag ttcagggtct gcagcagacg
     2821 gccgtcgcgg gtgtcgatca ggtagctctg ggtgctcggc cgacggccga caaacagcgc
     2881 catcggcaga aacgggtggc cgatcacatc gtggcagcgc tcgctgacct gagtggcgaa
     2941 tacctggctg ccatccagcc gatagccgac cgcgaagtgc ttgccggcac tgtcgttgcg
     3001 cgccgacagc agtagcggtt gagcgctact gtgcatcagt gtccagccac cgtaagtgcc
     3061 ggcggccgct gccgtagcgg caacggcagc gatgctcagc cctagaaagg cgcggcggtt
     3121 gatctgcatg gctcagtcac catcgtgagc gttaaagccc agttggatgc ccagggtctt
     3181 ggccagctcg gattcatgca ggcgatgcag cacattcagg ctgtcataca gggcgttgac
     3241 ttcagttcgg ccggcttcgt cgctaagcaa ttcgccgagc gggcgctggg cggcggctag
     3301 gcgctggcgg gtgtcactgt aggcggcgtt gatgcgttgt ttcagttcat cttgatcact
     3361 gccgagcagc gcctggatgc catcctgctc ggcacccagc cagatgcgtt cggcgctggc
     3421 caggctggcg gcgaggttgg ccaggctggc cttgctgcgc caggcttcgg cctgataggg
     3481 ctgcggttgg cctttgctct ggcggccgag tggcgtgccg agtttcttct tcagcccgtc
     3541 gatggcgctg acctgggtac gcagcagttc ggctaccgct tccggggctt cggcgtagcg
     3601 cgcgttgggg aagtccttga gcagcgcggc cataccgtcc ttggcctgcc actggctcag
     3661 cacatcggcg gccagcactt gctgatgctg gccgatggcg acgatcagcg ggcaatagcg
     3721 ggttttctgt tcagcgacgt tcaggtcgat ggacggatcg aacagcaggt attcataggc
     3781 ggtcaggcct tgcaccacga cgctggattt atccagatcg gctggggtga gttcgggttt
     3841 gctgttgatc agggcttcca cctgacgcgc caccaagttt ttcttgtccg gccagaactg
     3901 cacttgccag gcgcggttgc cttccgccag cgggccgaag gccactggct gcaaggcggc
     3961 ccaggcgctc tgcgcgctgc tgaatgccag gcgtgcatcg gtcaggctct gttgtccgct
     4021 gcagaaggcc tggccgctga cggccagttg ccggtcggct tcgctccagg cgctgtagat
     4081 cggcagcaac acgccttcgg taagtgcgcg gctgacctgc tgctgcggat tgctggggct
     4141 gcaggcagtg agcgtgaggc taagcagggc gagcgtgagg ggtgagcgga tcatctgcgg
     4201 ctccttacaa cgaattcaga aaagccaaca gcgcgctgcg ctcgtcggca ttgaaagtca
     4261 gcacctgctg cttggccgcc tcggcctcgc ctccgtgcca gagaatggcc tccagcaggt
     4321 tgcgtgcgcg gccgtcatgc aggaattggg tgtgaccgtt gaccgtttct gtcaggccga
     4381 tgccccacaa cggcggggtg cgccactcac tgccgctggc gagaaattca gggcgctggt
     4441 cggccaggtc tgcgcccatg tcatgcagca ataaatcgct gtaaggacgt atgacctgat
     4501 tggccagttc tggttcggcg gcgtcgctgg cggtggtgaa ctgcgggcta tgacagccct
     4561 ggcaaccggc ctggtagaac aggtttttgc cggcgagcac ctgcggtgta tccgcgccgc
     4621 gccgcgccgg cacgccgagg ttgcgggtgt agaacagcac gctggcgagg atgttgtcgc
     4681 tgacctcggg ctcgccgcca tggggggcgc tgcggcaatc cgtctgcgcc gttgtgcagt
     4741 tgtcatgggt caccagcgtg ctggtcaggc ccgtgtcgtt ggcaaaggct tcggcattct
     4801 gctgattgag cgtgggttgg ccggccttcc agccaaagcg gccgagcacc gtgcgttgtt
     4861 gctcgcggtc ccagacctgg ttggcgcgcc cggaaatacc atcgccattg ccgtcatccg
     4921 gatcggcatt ggccaggatg gctgcttcgg gaatggcttc gagcaggccc aggccgatca
     4981 tcggcggggc aatgcgcgcg gagaacaacg tatcaggatg cagatcgccg taacccagct
     5041 ggctgatttc caggctgggc ttgcgcagct cgacctgctg accatcggca aaactgacca
     5101 attgtgtgct gtaactgacg cgcaccttgc cttcgggcgt tacgcctggg ttggccatat
     5161 cctgcagctg agcgccgtag gtgggttcgg ccagcacgcc aaggcgctgg atgatttctg
     5221 aatgttcact gctagccggg atcgacagac gtaccagcat ggacgctgcg ctctgcgcat
     5281 caggggccgg tggatggccg cgaccgtcct tgatatggca gttctggcag gcgttggtgt
     5341 tgaacagcgg gccgaggcca tcacgcgcgg tggtggtggc cggcgaggtg acccaggggt
     5401 tgcgaaagaa gctgttgccg acgctgaaat ccaggcggcg tgaaggcgac aggttggctg
     5461 acggcaggga gaaggcgttc tgatccgcct tgcgcacggt ggcgctaccg gcggacaggg
     5521 cttcgccggg ctcggcctgg gtgaactgtg gggttttatc gcaggcaacc aggctcaggc
     5581 ccaaggccaa cagcagcgca atgcgatgcg gcgcaaacat gaaaggacat ccgcagcaga
     5641 aaaacaggcg gccgatctta acagtctgat cagttttaaa taagacggat ttgcattatc
     5701 ggtggtggtc aaaggtcgca ggtaataaaa agccgcgact ccattaagga gtcgcggctt
     5761 ttatcttggg cttcgcgagc tagagcgaga caagacagaa atggccgagg gcgcggagtt
     5821 tactggctgt aaatgagcag cccgaggcca tttctaacgc tgtatcgccg acgcgcagca
     5881 agtcctacgc gattagaagc tgtggtcagc ggtatctgga ttcagatcac tgatgcccag
     5941 cttgccagcg gcctgctcga ttgcgccggt ctgcttgacc agtgcggcga tggcgtcacg
     6001 aacgatctgc tggccttcgg cattgtcagc agcgatcagc tgatcgaaat gcttgccgtt
     6061 gttagcgctg tcgaccagag cctgcagctt gccttcagtg gcttcgaggt cggctttcag
     6121 ggtgctgtca gcagcggcgt caaccttggc caccagcgac gacaggcttg ggccggtcag
     6181 ggtggtgcca tcgaccttct tgtactcgcc taggtaaacg ttgcgaatac ctttgccgtt
     6241 gtagaagtgc gagttgtggg tgttatcgct gaagcagtcg tgctcgtctt cggtggagtt
     6301 ggcttccagc gcgactttca tgcgctcgcc ggccagttcg ccgagggaca ggctgcccat
     6361 gccgaagagc attttacgca ggccgttttc agccgtgtcg gcttccagcg tggcgcggta
     6421 gttgtcggtc ttgccagctt gccactgcac aaccattgca tcgaggtcgc tgaccagcag
     6481 gtctgcggcg gctttgataa aggcgcggcg gcgctcgttg ttgccgccag tggcgccttc
     6541 gcctacgacg aaatcgctgg ctgggcgttc gccggcgcct gggttggtgc cattcagatc
     6601 ttggccccac agcagaaatt cgatggcgtg gtagccggta gcgacgttgg cttcggagcc
     6661 gcccagctca ttcaggttgg ccagcagttc cggggtgatg gtggtgacgt cgagcttgtc
     6721 ttcgccgacc tgcagttcgg tgttggcgat gatgttggcg ctggcgcctg ggttacccag
     6781 ggcatgctgg tagtccttgg cgacgtagtc gatcaggcct tcgtccagcg gccaggcgtt
     6841 taactggcct tcccaatcgt cgaccacggc gttgccgaag cggaacactt cggtctgcat
     6901 gtaaggcaca cgggcagcca gccaggcttc gcgggcggct ttcagggtgt cgtcattcgg
     6961 attggcaagc agggcgtcaa tggcaccttg cagagttttg ccggtgctgg cggcgtcgct
     7021 gaatacggcc agggccaggt cggcgtagtg gctgaccact gctttgctgg cggcttcgtc
     7081 gacttggctg gtggcggcag ccggggcgct ggcgctgctg gcagctggtg cagcagcttg
     7141 ggtggctggc gctttgtctt caccgcaacc ggcgagtgaa atggcgatgg caagcaggct
     7201 ggcagaggcc aggggcatac ggagcatggc ggaatccttt gctggtgaga gtaagcaccc
     7261 acgcaagcgg gtacaaaaac cgaaacataa tgcgaaagat tttcattttg cgcaaagggt
     7321 caatgcagct ttt
//
LOCUS       DASRUM010000191         7350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_933640, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000191 DASRUM010000000
VERSION     DASRUM010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933640"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..116
                     /locus_tag="VFF22_12355"
                     /pseudo
     CDS             1..116
                     /locus_tag="VFF22_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284585.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="PrpF domain-containing protein"
     gene            complement(184..1002)
                     /locus_tag="VFF22_12360"
     CDS             complement(184..1002)
                     /locus_tag="VFF22_12360"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015269492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HZX17636.1"
                     /translation="MKRTAFFISDGTGITAETLGQSLLAQFETIDFTKLTRPYIDSVE
                     KARAMVQQINSASEKDGARPIIFDTIVNQDIRDVLAESDGYMIDIFSTFLAPLEQELT
                     SHSSYSVGKSHSIGHNSNYMERIEAVNFALDNDDGARTHYYDKADLILVGVSRCGKTP
                     TCLYMAMQYGIRAANYPLTEDDMERLQLPAVLKKHKDKLFGLTIDPDRLTAIRHERKP
                     NSRYASFAQCEFEVREVENLFRRENINFINSTYFSVEEISAKILVEKGVERRLK"
     gene            1180..3555
                     /gene="ppsA"
                     /locus_tag="VFF22_12365"
     CDS             1180..3555
                     /gene="ppsA"
                     /locus_tag="VFF22_12365"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HZX17637.1"
                     /translation="MVEYVVSLDKLGNHDVEHVGGKNASLGEMISNLASAGVSVPGGF
                     ATTAQAYRDFLELSGLNAQIHAALDALDVDDVNALAKTGAQIRQWVMDASFPEELNTQ
                     IRTAFAEMAGGNENMAVAVRSSATAEDLPDASFAGQQETFLNIRGVENVIRAAKEVFA
                     SLFNDRAIAYRVHQGFDHKLVALSAGVQRMVRSETGTAGVMFTLDTESGFRDVVFITG
                     AYGLGETVVQGAVNPDEFYVHKQTLEAGRPAILRRNLGSKAIKMIYGDEAKAGKSVKV
                     VDVERADRARFCLSDAEVSELAKQAMIIEKHYGRPMDIEWAKDGDDGKLYIVQARPET
                     VKSRTNVNVMERYLLKETGIVLVEGRAIGQKIGAGRVRVIHDVSEMDKVQPGDVLVSD
                     MTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATQVLKDGMGVTVS
                     CAEGDTGFIFEGELGFDIRKNSVDAMPDLPFKIMMNVGNPDRAFDFAQLPNEGVGLAR
                     LEFIINRMIGVHPKALLNFAGLPPEIKDSVEKRIAGYDDPVNFYVEKLVEGISTLAAA
                     FWPKKVIVRLSDFKSNEYANLIGGKLYEPEEENPMLGFRGASRYISESFRDCFELECR
                     ALKKVRNEMGLTNVEIMVPFVRTLGEASQVVELLAANGLARGQDGLKVIMMCELPSNA
                     ILAEEFLEFFDGFSIGSNDLTQLTLGLDRDSGIVAHLFDERNPAVKKLLSNAIQACNK
                     AGKYIGICGQGPSDHPDLARWLMEQGIESVSLNPDSVLDTWFFLAEGQAPA"
     gene            3698..4705
                     /locus_tag="VFF22_12370"
     CDS             3698..4705
                     /locus_tag="VFF22_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZX17638.1"
                     /translation="MQSSSELFPVALISAEFRGDLSEDVYRLKPGNSPDSSVELALTR
                     LGRAGHEQDRGVPVILIPGSFSNRRFWYSPKCIGLGPYLARVGFDVWIAEMRGHGLSP
                     RNRDYSRNTVSDYVRYDLPAIADFVHEQNPQKVHWLGHSLGGITLAGALGGHYLSESK
                     VASAVLFGSQVSRVYWPLKVPPLEWGSRLLLKRFSVISGSRLKRGPEDEPIGLAVESL
                     RWHGLFGRFGDAERDWWTGLADVRVPVMAVAALGDHQDPAWACEKLLKQFGSPLSEFL
                     CLGKKHGFSGDFGHVEMLVSKEAQREVWPLVEQWLRKSPQVAAELESRELEGDKAVSA
                     E"
     gene            4834..5322
                     /gene="rraA"
                     /locus_tag="VFF22_12375"
     CDS             4834..5322
                     /gene="rraA"
                     /locus_tag="VFF22_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HZX17639.1"
                     /translation="MHYITPDLCDAYPELVQVVEPMFSNFGGRDSFGGEIVTVKCFED
                     NSLVKEQVDLPGKGKVLVVDGGASMRRALLGDMLAEKAAKNGWEGIVVYGCVRDVDVL
                     AQTDLGIQALASHPMKTDKRGIGDLNVAVTFGGVTFRPGEFIYADNNGVIISPSALQM
                     PE"
     gene            5343..6338
                     /locus_tag="VFF22_12380"
     CDS             5343..6338
                     /locus_tag="VFF22_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015885348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HZX17640.1"
                     /translation="MHEGDNAQWGLVHAFVLNGQGGARSIARQELHGLVLGEQESLWL
                     HWDRSHPQTHTWLRTESGLSAFACDLLLEENTRPRLLALPEQQLLLFLRGLNLNPGAE
                     PEDMVSVRIFADTQRAISLRLRPLRATEDLIEDLLAGRGPKTSAELILRLAHYLTDKI
                     ERLVSELSEQVDDQEERADALESQAPDHSALLQIRRRAASLRRFLSPQRDIYGQLTAS
                     AQAWLVGADSSYWNELNNRLTRYLEELELSRERVNLLLEAENRRMDERMNHTMYRFGI
                     ITGIFLPMSFLTGLLGINVGGIPGSESPYGFLLACSLMALVALVQWLLFRRLRWI"
     gene            6393..6638
                     /locus_tag="VFF22_12385"
     CDS             6393..6638
                     /locus_tag="VFF22_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007940635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrfX protein"
                     /protein_id="HZX17641.1"
                     /translation="MHDPFEESLRDLLKSPTSSHDDDACLHRVLKTANRQVGAGDLFA
                     LMGHWAGALMLALNNGSAHVAPVSRRKTTDSTANKAD"
     gene            6641..>7350
                     /locus_tag="VFF22_12390"
     CDS             6641..>7350
                     /locus_tag="VFF22_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZX17642.1"
                     /translation="MEFTQVLVDAMASVWTPIAAFIPRLFGAMVVVLLGFVVAKFLDT
                     LLSKLLGKIGLDRLMAGTGLTKLLSRAGIQVPVSTLIGKIVYWFVLLIFLVTAAESLG
                     LERVSATLDVLALYLPKVFGAALVLLAGVLLAQLVSGLVRGAAEGVGLDYASGLGRVA
                     QGLVIIISISVAIGQLEVKTDLLNNVIAIVLISVGLAVALALGLGSRDIASQILAGIY
                     VRELYEVGQQVQVGAVEG"
BASE COUNT         1378 a         2016 c         2327 g         1629 t
ORIGIN      
        1 cgtgggtgcg caggcggcgc tggtcgacgg tcagtggacg aaccaaggcg attatgtcgc
       61 gtagcgcgcg gatattaatg gaaggttggg tgcgcgtgcc gggcgatatt ttgtagtgtt
      121 tataaatgag aaaaggctcg aagtattcga gccttttctt tttgcttcaa tgggttgcgc
      181 aggttactta aggcggcgct caacaccttt ttccaccagg atcttcgcgg aaatctcttc
      241 cacggaaaaa taggtggagt tgataaagtt gatgttctcg cggcggaaca ggttttccac
      301 ctcgcgtact tcgaactcgc actgggcgaa gctggcgtaa cggctattgg gcttgcgttc
      361 gtggcgaatg gcagtgaggc gatccgggtc gatggtcagg ccgaacagct tgtccttgtg
      421 ttttttcagc accgcaggca actgcagtcg ctccatatca tcttcggtca acggataatt
      481 tgcggcacga attccgtatt gcattgccat atacaggcaa gtcggggttt tgccgcagcg
      541 cgacacgccg accaggatca gatcggcctt gtcgtagtag tgggtgcgtg cgccgtcgtc
      601 gttgtccagg gcgaagttca ccgcctcgat acgctccata tagttggaat tgtggccgat
      661 ggagtgggac ttaccgacgg agtaggacga atgcgaggtc agttcctgct ccaggggggc
      721 gaggaaggtc gagaagatat cgatcatata accatcggat tcggccagga cgtcgcggat
      781 atcctgattg acgatggtgt cgaagatgat cgggcgggca ccgtccttct cggacgcact
      841 gttgatttgt tgtaccattg cgcgggcttt ttccacgctg tcgatataag gccgcgtgag
      901 tttggtgaag tcgatggttt caaactgcgc cagcaggctt tgacccaggg tttcggcagt
      961 gatgccggtg ccgtcggaga tgaaaaaagc ggttcgtttc atttgcacca tgggccttaa
     1021 gctgggaagg attcttggct atcataggcc cgcctttaca gggccgaact ggctggcatt
     1081 gttacttatt ttccagggcc aggccacaat cgtgcgggca tgtaccgccg aaccatctag
     1141 ctcctgagtt tttccaacac cgttagtgga gagatcacct tggtagagta cgtagtttcc
     1201 ctcgataagc tcggcaatca tgatgttgag cacgtagggg gcaagaacgc ctccctgggc
     1261 gagatgatca gcaatcttgc cagtgccgga gtgtcggtgc ccggtggttt cgccactacc
     1321 gcccaggcct atcgggactt cctcgagctg agcggcctga atgcgcagat ccatgcggcc
     1381 ctggatgccc tcgacgtgga tgacgtcaat gccctggcca agaccggtgc acagatccgc
     1441 cagtgggtga tggatgcctc cttccccgaa gagctgaaca cgcagatccg caccgccttc
     1501 gccgaaatgg ccggcggcaa cgagaatatg gcggttgccg tgcgctcctc ggccaccgcc
     1561 gaagacctgc cggacgcttc ctttgccggc cagcaggaaa ccttcctgaa tatccgcggc
     1621 gtggaaaacg tgatccgcgc ggccaaagag gtattcgcct cgctgttcaa cgaccgcgcc
     1681 attgcctacc gcgtgcatca gggcttcgac cataaactgg tcgccctgtc tgccggtgtg
     1741 caacgcatgg tgcgttctga aaccggcacc gccggcgtga tgttcaccct ggacaccgag
     1801 tctggcttcc gtgatgtggt gtttatcacc ggcgcctacg gcctgggtga aaccgtggta
     1861 cagggtgcgg ttaaccctga tgagttctat gtccacaaac aaacgctgga agccggccgc
     1921 ccggcgatct tgcgccgcaa cctgggcagc aaagccatca agatgattta cggcgacgaa
     1981 gccaaggccg gtaaatcggt caaggttgtc gatgtggaac gcgccgatcg cgcgcgcttc
     2041 tgcctcagcg atgccgaggt cagcgagctg gccaagcagg cgatgatcat cgagaagcac
     2101 tacggtcgtc cgatggacat cgagtgggcc aaagacggcg acgacggcaa gctgtacatc
     2161 gttcaggcgc gtcctgaaac ggtgaaaagc cgcaccaatg tcaatgtaat ggagcgctac
     2221 ctgctcaagg aaaccggcat cgtgctggtg gaaggtcgcg ctatcggtca gaagatcggt
     2281 gcaggccgcg tgcgggtcat ccatgacgtg tccgagatgg acaaagtgca gcccggcgac
     2341 gtgctagtct ccgacatgac cgacccggac tgggaaccgg tgatgaagcg tgccagtgcc
     2401 atcgtcacca accgtggcgg ccggacctgc cacgcggcga tcatcgcccg tgagctgggt
     2461 attcctgcgg tagtcggctg cggcaacgcc acccaggtgc tgaaagacgg catgggtgtg
     2521 acggtttcct gcgctgaggg cgacactggc tttatttttg aaggcgagct gggcttcgat
     2581 atccgcaaga attcggtcga tgccatgccg gatctgccgt tcaagatcat gatgaacgtc
     2641 ggtaaccccg atcgcgcctt cgacttcgcc cagctgccca acgaaggtgt tggcctggcg
     2701 cgtctggaat tcatcatcaa ccgcatgatc ggcgtgcacc cgaaagcgct gttgaacttc
     2761 gccggcctgc cgccggagat caaggacagc gtcgagaaac gcatcgccgg ttacgacgat
     2821 ccagtcaatt tctacgtcga gaaactggtc gagggcatca gtactctggc tgcggcgttc
     2881 tggccgaaga aagtcatcgt gcgtctgtcg gacttcaagt ccaacgaata cgccaacctg
     2941 atcggcggca agctgtatga gccggaagaa gaaaacccga tgctgggttt ccgtggtgct
     3001 tcgcgttaca tcagcgaatc gttccgcgac tgcttcgagc tggagtgccg cgcgctgaag
     3061 aaagtgcgca acgagatggg cctgaccaac gttgagatca tggtgccgtt cgtgcgcacc
     3121 ctcggcgaag ccagtcaggt ggtcgagctg ctcgccgcca acggcctggc ccgtggtcag
     3181 gatggcctga aggtcatcat gatgtgcgaa ctgccgtcca acgcgattct ggctgaagaa
     3241 ttcctcgaat tcttcgacgg cttctccatc ggctccaacg acctgaccca gctgactttg
     3301 ggcctggacc gtgactctgg catcgtcgcg cacctgttcg atgagcgtaa cccggcggtg
     3361 aagaagctgc tgagcaacgc catccaggcc tgtaacaagg ctggcaagta catcggtatc
     3421 tgcggccaag gcccttcgga tcacccggat ctggcgcgtt ggttgatgga gcagggcatc
     3481 gagagcgtgt cgctgaaccc ggattcggtg cttgatacct ggttcttctt ggccgaaggt
     3541 caggcgccag cctgatagcg ctttaaaatc tctgttcaag ggcgggttaa tcctgccctt
     3601 ttttgtgcca gcacatacct gtgcttaccc ctccgggctg tcgcttgcga cgtgaaagaa
     3661 ttgctcccgg caattggttg taccagagcg aagatagatg caaagcagca gtgagctttt
     3721 ccccgtcgct ttgatcagcg cggagttccg tggcgatttg agcgaggatg tctatcgcct
     3781 caaacccggt aacagtccgg acagcagtgt tgagctggcc ctgactcgcc ttggtcgtgc
     3841 cgggcatgag caggatcgcg gcgtgccggt gattctgatt cctggcagtt tttccaaccg
     3901 ccgtttttgg tattcgccaa aatgcattgg cctggggcct tacctggcgc gcgtcggttt
     3961 tgatgtatgg atcgccgaaa tgcgcggcca cggtttatcg ccgcgtaacc gcgactattc
     4021 acgcaacacg gtcagtgatt acgtgcgcta cgacttgccg gcgattgccg attttgtcca
     4081 tgagcagaat ccgcaaaagg tccattggct gggtcattcg ctcggcggga ttaccctcgc
     4141 cggcgcgctc ggcgggcatt acctgagtga aagcaaagtc gcctccgctg tgttgttcgg
     4201 cagccaggtc agccgggttt actggccgct gaaggtgccg ccgctggagt ggggcagccg
     4261 cttgctgctc aagcgtttct cggtgatttc cggctcgcgg ctcaagcgtg gccccgagga
     4321 tgagccgatt ggtctggcgg tcgagagtct gcgctggcat ggtttgttcg gccgttttgg
     4381 cgacgctgag cgcgattggt ggactggctt ggccgacgtg cgggtacctg ttatggccgt
     4441 ggccgcgcta ggtgatcatc aggatcctgc ctgggcctgc gagaagttgc tcaagcagtt
     4501 cggttcgccc ttgagcgagt ttctctgcct gggcaaaaaa catggcttta gcggtgattt
     4561 cggccatgtc gaaatgctcg tcagcaaaga agcgcagcga gaggtctggc cgctggtcga
     4621 gcaatggctg cgcaagagcc cgcaggtggc tgcagagctg gaaagccgcg agctagaagg
     4681 ggataaagcc gtttccgctg agtgagcttg cggctagtat cgtggttcag gtttgcgccc
     4741 tgcgccagtc ttgtgtattt gcctgtggct tatgatgcgg atcgggtcgg gatcgacctg
     4801 taagcaccct cacactatcc aaggagcttt gccatgcact acatcactcc cgatctgtgc
     4861 gacgcctacc cggaactggt ccaggtggtt gagccgatgt tcagcaactt cggtggtcgc
     4921 gactctttcg gtggcgagat cgtcaccgtc aagtgcttcg aggacaactc gctggtcaag
     4981 gagcaggttg atctgcccgg taaaggcaaa gtgctggtag tcgacggtgg tgcctccatg
     5041 cgccgcgcgc tgctgggcga catgctggcc gagaaagccg cgaagaatgg ttgggaaggc
     5101 attgtggtgt atggctgcgt gcgtgatgtc gatgtactgg cgcagaccga tctgggcatc
     5161 caggccctgg ccagtcaccc gatgaaaacc gacaagcgcg gcatcggtga cctcaatgtc
     5221 gcagtgacct tcggcggcgt aaccttccgt ccaggcgagt ttatctatgc cgacaataac
     5281 ggcgtgatca tctcgccatc tgcgctgcag atgccggagt agggcgcttg caggaaactg
     5341 tcatgcacga aggtgacaac gcgcagtggg gcctggtgca cgcctttgtc cttaatggtc
     5401 agggcggtgc ccgcagcatt gcccggcagg agctgcacgg gctggtgttg ggcgagcagg
     5461 aaagcctctg gctgcactgg gatcgcagtc acccgcaaac gcacacctgg ctgcgtacag
     5521 agagtggcct gagcgctttt gcctgcgatc tgctgctgga agagaacacc cgtccgcgtt
     5581 tgctggcgtt gcctgaacag caattgctgc tgtttctgcg tggcctgaac ctcaacccag
     5641 gggcggagcc tgaggacatg gtctcggtgc gtatcttcgc ggatacccag cgggcgattt
     5701 ccctgcgctt acggccgttg cgcgcgactg aggacttgat cgaggatctg ctggcggggc
     5761 gggggccgaa aacctcggct gagctgattc tgcgtttggc gcattacctg accgacaaga
     5821 tcgagcggct ggtcagtgag ctgtccgagc aggtcgatga tcaggaagag cgcgccgatg
     5881 cgctggaaag tcaggcgcct gaccattccg cgctgctgca aatccgccga cgcgccgcca
     5941 gcctgcggcg ttttctctcg ccacagcggg atatctacgg acagcttacg gccagtgccc
     6001 aggcctggct ggtgggggct gacagcagct actggaacga gctgaataac cgcctgacgc
     6061 gttacctcga agaactggag ctgagccgtg aacgggtcaa cttactgctg gaggcggaaa
     6121 atcggcgcat ggatgagcgg atgaaccaca ccatgtaccg cttcggcatc atcaccggaa
     6181 tattcctgcc gatgagcttt ctcaccggct tactcggtat caatgtcggc ggtattccgg
     6241 gctccgaaag cccttatggg tttctgctgg cgtgttcgct gatggcgctg gtagcacttg
     6301 tgcaatggtt actgtttcgt cgcttgcgct ggatctgatg tgacttgccc cgtactcacc
     6361 tcgtctagcc gtcacatcct gtgaggtgcg ccatgcacga tccctttgaa gaatcactgc
     6421 gcgatttgct caaatcgccc acctcaagtc acgacgatga tgcttgcctg catcgtgtgc
     6481 tgaaaaccgc gaaccgccag gtcggcgctg gtgatctgtt tgccctgatg gggcactggg
     6541 caggcgcact gatgctggcg ctgaataacg gctctgcgca tgtggcgccg gtttcgcgtc
     6601 gtaaaactac tgattcaact gctaataagg ctgactgagc atggaattta ctcaagttct
     6661 ggttgatgcg atggccagcg tttggacccc gattgccgcc tttatcccgc gcctgtttgg
     6721 cgccatggtg gtggtactgc tgggtttcgt ggtggccaag ttcctcgaca cgctgctgtc
     6781 caagttgctc ggcaaaattg gcctggatcg cctgatggca ggtacgggcc tgaccaagct
     6841 gctgagccgt gccggcattc aggtgccggt atcgaccctg atcggcaaga tcgtctactg
     6901 gttcgtgctg ttgatcttcc tggttacggc cgctgagtcc ctggggctgg agcgggtttc
     6961 cgcgacgctt gatgtgctgg ccttgtacct gccgaaagtc ttcggcgcgg ccctggtgtt
     7021 gctcgctggc gtgctgctgg cgcaactggt cagcggcctg gtgcgcggcg ccgccgaagg
     7081 cgttgggctg gactatgcaa gtgggctggg tcgcgttgct cagggcctgg tgatcatcat
     7141 cagtatctcg gtggcgatcg gtcagcttga agtgaaaacc gacctgctca acaacgtgat
     7201 cgccatcgtg ctgatctcgg tgggtctggc ggtagccctg gcgctgggtc tgggcagtcg
     7261 ggatattgcc agccagattc tggccggcat ttatgtgcgc gagttgtatg aggttgggca
     7321 acaagtgcag gtcggtgcgg tcgaagggca
//
LOCUS       DASRUM010000192        54374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_435163, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000192 DASRUM010000000
VERSION     DASRUM010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 54374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54374
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435163"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFF22_12395"
     CDS             <1..253
                     /locus_tag="VFF22_12395"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HZX17643.1"
                     /translation="EGVDFILINPAAFTHTSVALRDALLAVSIPFIEVHLSNVHKREA
                     FRHHSYFSDVAVGVICGLGASGYRLALEAALEQLELKRP"
     gene            276..737
                     /gene="accB"
                     /locus_tag="VFF22_12400"
     CDS             276..737
                     /gene="accB"
                     /locus_tag="VFF22_12400"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HZX17644.1"
                     /translation="MDIRKVKKLIELLEESGIDELEIREGEESVRISRHSKQPAFMQQ
                     PMYAQAPAPVAAPVAAAPVAAEAAAPAAAKLNGTVVRSPMVGTFYRASGPTSGNFVEV
                     GQSVKKGDILCIVEAMKMMNHIEAETSGVIESILGENGQPVEYDQPLFTIV"
     gene            755..2104
                     /gene="accC"
                     /locus_tag="VFF22_12405"
     CDS             755..2104
                     /gene="accC"
                     /locus_tag="VFF22_12405"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HZX17645.1"
                     /translation="MLEKVLIANRGEIALRILRACKELGIKTVAVHSTADRELMHLGL
                     ADESVCIGPATGALSYLNIPAIISAAEVTGATAIHPGYGFLAENADFAEQVENSGFAF
                     VGPKAETIRLMGDKVSAKHAMIKAGVPVVPGSDGPLPEDEETALQIAREVGYPVIIKA
                     AGGGGGRGMRVVYKEEDLIKSAKLTRSEAGSVFGNPMVYLEKFLGNPRHVEVQVLSDG
                     QGNAIHLGDRDCSLQRRHQKVLEEAPAPGIDEKARAEVLQRCVDACIEIGYRGAGTFE
                     FLYEDGHFYFIEMNTRVQVEHPVSEMVTGIDIVKEMLSIAAGNKLSFTQEDVVIRGHS
                     LECRINAEDPDNFMPCPGKVKHFHAPGGNGVRVDSHLYSGYTVPPHYDSLIGKLITYG
                     ATRDEALARMRNALDEIVVDGIKTNVPLHRMLVRDKGFCKGGVNIHYLEKKLGMDKH"
     gene            2248..3126
                     /gene="prmA"
                     /locus_tag="VFF22_12410"
     CDS             2248..3126
                     /gene="prmA"
                     /locus_tag="VFF22_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HZX17646.1"
                     /translation="MPWLQVRLAITPEQAETYEDALLEVGAVSVTFMDAEDQPIFEPD
                     LGTTPLWSHTHLLALFEADTDAGAVFAHLQLLTGTDLPEHQAEVIADQDWERSWMDGF
                     HPMRFGQRLWIVPSWHAAPEPDAVNLLLDPGLAFGTGTHPTTALCLEWLDGQPLQDCS
                     VIDFGCGSGILAIAALLLGAPQAVGTDIDIQAIEASRDNAGRNGIDPARFPLYLPDDM
                     PQQPADVVVANILAGPLVALAPQITSLVKPGGRLALSGILAEQADEVRAAYNDAFILD
                     PTADKDGWVRISGVRR"
     gene            3203..4540
                     /locus_tag="VFF22_12415"
     CDS             3203..4540
                     /locus_tag="VFF22_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HZX17647.1"
                     /translation="MTQSFVTQCPHCRTSFRVNLTQLGAAHGAVRCGACLHVFNAAQQ
                     LREQGQQLPGPATPAAAPTPAPQPSKPPVPPAPRPAMPSAAPAIPAAPASSKTGDTLW
                     IHDDLDLDSLDLDEELAKLEAQEQQLSKQFLAIDSAPKYNESFLPAEPIEHDPHDERW
                     AEALLQDELSKPATSLSVKPQPLTPPVRPTPPPAPVTPEPINEPDDEPPLDLNALDRR
                     HPEPEIEHIELHAEREPFSALTDNPADEEPAVAAPKKTARSEPELRDEHLFELDDEPL
                     QLDWQQPKKPWGRWIGWGLLNLLGAAALAGQYVMYHFNELARQDQYRPWFEQLCPAVG
                     CQLPSKVDITQVKSSNLVVRSHPEFSGALVVDAILYNRAAFSQPFPLLEMRFADINGQ
                     LLASRRFKPSEYLAGELAGNAEMPPQTPIHISLDILDPGTQAVNYSLSFHSPE"
     gene            4720..5718
                     /gene="dusB"
                     /locus_tag="VFF22_12420"
     CDS             4720..5718
                     /gene="dusB"
                     /locus_tag="VFF22_12420"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HZX17648.1"
                     /translation="MSALRIGPYTLPNSLILAPMAGVTDQPFRQLCKRMGAGLVVSEM
                     VTSDVRLWNTRKSSLRMIHSGDPEPRSVQIAGGDPEMLAEAARRNVEMGAQIIDINMG
                     CPAKKVCNKAAGSALLKDETLVREILQAVVAAVDVPVTLKIRTGWDRENKNGIAVAKI
                     AEDAGIVALAVHGRTRADLYMGEAEYDTIAAIKQAVSIPVLANGDIDSPQKAKAVLAA
                     TGADGLLIGRAAQGRPWIFREVEHYLRTGEQLPAPGLREVERILLEHLAALHAFYGDV
                     MGVRIARKHVSWYLATLPGAREFRAHFNRLDSTDAQCANVREFFSERHNNGEGVAA"
     gene            5715..6035
                     /gene="fis"
                     /locus_tag="VFF22_12425"
     CDS             5715..6035
                     /gene="fis"
                     /locus_tag="VFF22_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012274390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HZX17649.1"
                     /translation="MTMLTETLESGMAPVSDNSSLKQHLNTPSEEGQTLRGSVEKALH
                     NYFAHLEGADVCDVYNLVLTEVEAPLLETVMNYVKGNQTKASELLGLNRGTLRKKLKQ
                     YDLL"
     gene            6126..7733
                     /gene="purH"
                     /locus_tag="VFF22_12430"
     CDS             6126..7733
                     /gene="purH"
                     /locus_tag="VFF22_12430"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZX17650.1"
                     /translation="MTDQTTRLPVRRALISVSDKTDILEFARELVALNVEILSTGGTY
                     KLLKDNGIAAVEVADYTGFPEMMDGRVKTLHPKIHGGILGRRSIDGAVMDEHGIKPID
                     LVAVNLYPFAATVAKPGCDLADAIENIDIGGTTMVRSAAKNHKDVAIVVNAGDYAGIV
                     ESLKAGGLSYAQRFDLALKAFEHTAAYDGMIANYLGTIDQSRDTLSTEERGAFPRTFN
                     SQFIKAQEMRYGENPHQSAAFYVETQKGEASVASAIQLQGKELSFNNVADTDAALECV
                     KSFVKPACVIVKHANPCGVAVALDSEGGIRQAYELAYATDTESAFGGIIAFNRELDGA
                     TAQAIVERQFVEVIIAPKISAEARAIVAAKANVRLLECGEWPAERSAGWDFKRVNGGL
                     LVQSRDIGMIKAEDLKIVTKRAPSEQEIHDLIFAWKVAKFVKSNAIVYAKGRQTIGVG
                     AGQMSRVNSARIAAIKAEHAGLQVAGSVMASDAFFPFRDGLDNAAANGITAVIQPGGS
                     MRDAEVIAAADEANIAMVFTGMRHFRH"
     gene            7932..9221
                     /gene="purD"
                     /locus_tag="VFF22_12435"
     CDS             7932..9221
                     /gene="purD"
                     /locus_tag="VFF22_12435"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZX17651.1"
                     /translation="MNVLIIGSGGREHALAWKVAQDKRVNKVFIAPGNAGTATEAKCE
                     NVAIDVLAIEQLADFAEKNVQLTIVGPEAPLVKGVVDLFRTRKLDIFGPTAAAAQLEG
                     SKAFTKDFLARHAIPTADYQNFTEVEPALTYLQKVGAPIVIKADGLAAGKGVIVAMTL
                     QEAEDAVRDMLAGNVFGEAGSRVVIEEFLDGEEASFIVMVDGENVLPMATSQDHKRVG
                     DADTGPNTGGMGAYSPAPVVTADVHQRVMAEVIYPTVRGMASEGNVYTGFLYAGLMID
                     KAGSPKVIEFNCRFGDPETQPIMCRLESSLVLLIEAALAKALDKVEATWDPRPTVGVV
                     IAAGGYPGDYAKGDVIEGLDAAAQLEGKVFHAGTALKDGQIVTAGGRVLCATAIGRTV
                     EEAQQQAYRLAEKIRWSGSFYRNDIGYRAIARERGDK"
     gene            9383..12115
                     /locus_tag="VFF22_12440"
     CDS             9383..12115
                     /locus_tag="VFF22_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX17652.1"
                     /translation="MALTVASAQAGAQASWSSLTDPSAELQFNDVRSPERQAQFRSTD
                     LSQLYTPGGNSALWLHHRMPANTDAQMLRVFAPYLAYLDLYVLQGDTLIEQAHTGSNL
                     PFSSRPLASRDFLLPLPKAEQPLDIYLRLASEHALRPSITLQSAQVMVADDNRPLLFG
                     LLLGCFGMLVAYNLVRFAYTRSVSGLWLAATQVCQLVAVVSLLGISAPWLSEWQSLQP
                     QIANLSMLLAALCALRFTASFFHKVCPSTPLNHLLTAEVVVISLVCVALLVATNLQFN
                     QLVYLLNAVAGLSILLVALSHWRHGYQPARLFSLAVLLFCAAFICALPILFGYWAVQS
                     EWMAYGLLTITAVSGFILSMALSERQRRIMQDQFSTSRALAASSAELKAKAEFLAKIS
                     HEIRTPMNGVLGMTELLLGTPLSAKQRDYVQTIHSSGNELLTLINEILDISKLESGQI
                     ELDDVQFDLNALIEDCLDIFRAKAEQQKVELISFMQPQVPRVISGDPTRLRQTLLSLL
                     DNAFKQTDEGEILLVVALDTNTEQPRLRIAVQDSGKPLDASERDALLNAELHSKDFLA
                     ATKLGGRLGLIIARQLVRLMEGEFGIQSGGSQGSTLWLTLPLDATRLEQPTADLDASL
                     QGARLLVVDDNDTCRKVLVQQCSAWGMQVSAVPSGKEAMALLRTKAHLREYFDAVLLD
                     QDMPGMTGMQLAARIKEDANLNHDILVIMLTGISNAPSKIIARNAGIKRILAKPVAGY
                     TLKTTLADELAQRGKGDSPMFNPAPQSTPLNVPTDFRILVAEDNTISTKVIRGMLGKL
                     NVQPDTASNGEEALSAMKAQQYDLVLMDCEMPVLDGFSATEQLRAWEAAEQRVRTPVV
                     ALTAHILNEHKERARQVGMDGHMSKPVEMSQLRELIEHWVAEREIRRQRDALPS"
     gene            12191..12787
                     /locus_tag="VFF22_12445"
     CDS             12191..12787
                     /locus_tag="VFF22_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HZX17653.1"
                     /translation="MASALFSLYLKLLVLYSPFFVLSCFIGLSRGYTVKERKRLAWKV
                     ALGVLIASVLLYLFGKHIFTLFGITIDAFRIGAGSVLFISALGMAQGKSAVQSDNVQQ
                     DVTIVPLTIPLTVGPGTIGALLLMGASQPDWGDKAVAVLGIALASLTVGVVLYMSNQF
                     ERLLGDQGLQIVSRLMGLFVCALAAQIIFTGVKNYLAL"
     gene            12980..14782
                     /gene="atzF"
                     /locus_tag="VFF22_12450"
     CDS             12980..14782
                     /gene="atzF"
                     /locus_tag="VFF22_12450"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HZX17654.1"
                     /translation="MSKAPIAFTLADWQQTYRDGQSPAELLHSLRLELSTEDNAWIAL
                     TSDAQLNAQLDSLSTLLASSGGELSKLPLYGVPFAIKDNIDAAGWPTTAACPEFAYTA
                     AADATVVAKLRAAGAILIGKTNLDQFATGLVGTRSPHGAVCNSFNPDYVSGGSSSGSA
                     SVVARGLVPFSLGTDTAGSGRVPAGFNNIVGLKPSKGWLSNNGLVPACKTLDCISIFA
                     LTVSDALSVAQIAGGYDAADPYSRKNPCSAKVGMPAQPRLAVPSNPEFFGDTQNQAVF
                     ERALGKLRELDVQLVEIDFAPFQQLAEQLYYGSWVAERTVAVEGVNPDHINPVVRGIV
                     KNGHKYSACDAYKAEYIRAELSRKINDVLAGFDALVVPTSPTIRSLAEMDAEPVLFNS
                     QFGTYTNFTNLADLSALAVPAGMRADGLPAGITLIAPAWHDQALSDFGQRWQQALNLP
                     LGATGKALPAQVASSSPAPGCVRVAVVGAHLTGMPLNFQLTSRDAVLVEQTSSAATYR
                     LYALPGTVPPKPGLARVAEDGAAIIVELWDIPQARFGEFVAEIPAPLGIGNLQLADGR
                     WVKGFICEPYALAGARDITSFGGWRAFIASQQQG"
     gene            14787..15518
                     /locus_tag="VFF22_12455"
     CDS             14787..15518
                     /locus_tag="VFF22_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZX17655.1"
                     /translation="MQLSGTPGTGKERPENLAERIYQQLKDDIFEFRLLPGDRFSEGE
                     IAERMAVSRTPVRQALYRLQREDYLQVYFRSGWQVKPFDFAHFEELYEVRIVLEMEAV
                     RRLCERPQDEHCAALEQLKRTWLVQPEQRLQDGREVSRLDERFHCLLVEATGNREMAR
                     LHAEVSEKIRIIRRLDFTQGPRVTATYEEHGQILAAILSRRCEEAQLLLKTHIEVSKA
                     EVRKITLHMLHSARQRTMPAPVVKT"
     gene            15616..16881
                     /gene="urtA"
                     /locus_tag="VFF22_12460"
     CDS             15616..16881
                     /gene="urtA"
                     /locus_tag="VFF22_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HZX17656.1"
                     /translation="MQRRNLIKAFTLSASIAAMGMTWTVQAAETIKVGILHSLSGTMA
                     ISETSLKDMALMTIDEINAKGGVLGKQLEPVVVDPASNWPLFAERGRQLLTQDKVDVT
                     FGCWTSVSRKSVLPVYEELNGLLFYPVQYEGEEMSPNVFYTGAAPNQQAIPAVEYLMS
                     EDGGAAKRYFLLGTDYVYPRTTNKILRAFLISKGVAEKDIEEVYTPFGHSDYQTIVAN
                     IKKFSAGGKTAVISTVNGDSNVPFYKELANQGIEATDIPVVAFSVGEEELRGIDTKPL
                     VGQLAAWNYFQSVENPVNTKFVADWKAYAKAKNLPNADTVVTNDPMEATYVGIHMWAQ
                     AVEKAGTTDVDKVREAMYGQTFAAPSGYTLTMDKSNHHLHKPVMIGEVQEDGQFSVVW
                     ETSSPVRAQPWSPYIEGNDKKPDYAVKSN"
     gene            17012..18583
                     /gene="urtB"
                     /locus_tag="VFF22_12465"
     CDS             17012..18583
                     /gene="urtB"
                     /locus_tag="VFF22_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HZX17657.1"
                     /translation="MPTAFTRILLSLLLLLPMAAHAGDAQDFVAASPAKQASLLESWS
                     AAPDAARLPLLEALQQGRVAADSTKTAFIEVGGVYQPAEGEAVPTEAPRKLRLNNRLR
                     GLISTAVASHQLLAEDPALRLAAAQQLQKSAKPAQLQLLNAQVASETDDAVRDALTLA
                     LANLQLVDSDLAVRLAAVRLLGETGDPLARTRLEALLDPSVESDPTVRTAAETSLAQV
                     KRKLLIGEILGQAFSGLSLGSILLLAALGLAITFGLLGVINMAHGEMLMLGAYSTYMV
                     QVLFQRFAPEALALYPLVALPVAFFVTAAIGMALERTVIRHLYGRPLETLLATWGISL
                     ILIQLVRMIFGAQNVEVANPYWLSGGIQVLPNLVLPYNRIVIIGFALFVVLLTWLLLN
                     KTRLGLNVRAVTQNRNMAACCGVPTGRVDMMAFGLGSGIAGLGGVALSQIGNVGPDLG
                     QSYIIDSFLVVVLGGVGQLAGSVMAAFGLGIANKILEPQIGAVLGKILILALIILFIQ
                     KRPQGLFALKGRVID"
     gene            18713..19804
                     /gene="urtC"
                     /locus_tag="VFF22_12470"
     CDS             18713..19804
                     /gene="urtC"
                     /locus_tag="VFF22_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HZX17658.1"
                     /translation="MTMPLNQTLLARASAKLGPRVSIAIGLLVLTLLIAMPLLHLLPA
                     DNALHVSAYTLTLVGKILCYCIVALALDLVWGYAGLLSLGHGLFFALGGYAMGMYLMR
                     EAAGDGLPAFMSFLAWTELPWYWYGTSNFLWALCLVVLAPGLLALVFGFFAFRSRIKG
                     VYFSIMTQALTFAGMLLFFRNETGFGGNNGFTNFRTILGFDITSAHTRAVLFLATVVL
                     LVASLYLGWTLARSKFGRVLTALRDAENRLMFCGYDPRGYKLFIWVLSAVLCGLAGAL
                     YVPQVGIINPSEMSPTNSIEAAVWVALGGRGTLIGPLLGAGLVNGMKSWFTVAFPEYW
                     LFALGFLFIVVTLFLPKGVIGLLRKKGEQ"
     gene            19804..20655
                     /gene="urtD"
                     /locus_tag="VFF22_12475"
     CDS             19804..20655
                     /gene="urtD"
                     /locus_tag="VFF22_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HZX17659.1"
                     /translation="MRATPVPEFMLEPAFDPSGSGRDAIGASTLASKGLNVRHGTILT
                     LEDINVSFDGFKALTNLTLYIGVGELRCIIGPNGAGKTTMMDVITGKTRPDNGVAYFG
                     ETLDLTQMSEVEIAQSGIGRKFQKPTVFEALSVFENLELAQKTNKSVWASLRAKLSGE
                     QKDRIDEVLQTIKLDASRNRPAGLLSHGQKQFLEIGMLLVQDPQLLLLDEPVAGMTDA
                     ETEFTAELFKSLARKHSLMVVEHDMGFVGSIADHVTVLHQGSVLAEGSLEQVQADERV
                     IEVYLGR"
     gene            20757..21455
                     /gene="urtE"
                     /locus_tag="VFF22_12480"
     CDS             20757..21455
                     /gene="urtE"
                     /locus_tag="VFF22_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HZX17660.1"
                     /translation="MLQVQQLHQYYGGSHILRGLSFEAKVGEVTCLLGRNGVGKTTLL
                     KCLMGLIPAKQGAVSWEGQPITAFKPHQRVHAGIAYVPQGREIFGRLTVEENLLMGLS
                     RFPGSEAKVVPEFIYELFPVLREMKQRRGGDLSGGQQQQLAIGRALASKPRLLILDEP
                     TEGIQPSVIKEIGVVIKKLAERGDMAILLVEQFYDFAAELADQYLVMSRGEIVQQGRG
                     ETMETDGVRGLVAI"
     gene            21519..21935
                     /locus_tag="VFF22_12485"
     CDS             21519..21935
                     /locus_tag="VFF22_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17661.1"
                     /translation="MPPLRTLLLPLTALVGMALVIWAGSLPDYWMLRAMPVGSEAVYP
                     LKPVLIFCAIVLAECSVLLAVLRPASYCRSWGRALCASVLALGLALIWIQGILHAPPY
                     YGMHLQWWLAVSLGLILLSAYSAVQAWRNRSSRVTA"
     gene            21932..22765
                     /locus_tag="VFF22_12490"
     CDS             21932..22765
                     /locus_tag="VFF22_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HZX17662.1"
                     /translation="MNAPAVLFTPSWHAELELGYGRDGETTRPTLRRHKGPLRVQKHL
                     YAEGPQVCQHIIVHPPGGIAGGDRLDISASVGSGAWAQLTSPGAAKWYRAAGPAYQSL
                     KLRVEAGATLEWLPQETIVYSAAQAELTTDIELLGDAKLLYWDIVALGRPASGERFDA
                     GHFKAQLNIRRDGQLLWHERQRVVGGDGLLDSPIGLDGKPVFATLLISGEIDSELLEH
                     CRSLNTPVRGDLTQLPGLLVARCLADEALHARAWLIELWHLLRPALLGRTAVPPRIWS
                     T"
     gene            22765..23067
                     /gene="ureA"
                     /locus_tag="VFF22_12495"
     CDS             22765..23067
                     /gene="ureA"
                     /locus_tag="VFF22_12495"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HZX17663.1"
                     /translation="MDLTPREKDKLLIFTAGLVAERRLARGVKLNYPEAMAYISAALL
                     EGARDGQTVAELMHFGTTLLSRDQVMEGIAEMIPEIQVEATFPDGTKLVTVHQPIA"
     gene            23223..23735
                     /locus_tag="VFF22_12500"
     CDS             23223..23735
                     /locus_tag="VFF22_12500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX17664.1"
                     /translation="MIIRDARDDDLEAIRAIFNDAVLNTTAIWMDDIVDLANRQAWLA
                     ARRQQGYPVLVAVDADDSVLGYAAYGDWRPFDGFCNTVENSLYVRADQRGRGLGVLLL
                     GALIKRGKAGGKHVMVAAIESGNLASIGLHQRFGFSITGQMPQVGRKFGRWLDLTFMQ
                     LTLSPERSAP"
     gene            23732..24037
                     /locus_tag="VFF22_12505"
     CDS             23732..24037
                     /locus_tag="VFF22_12505"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HZX17665.1"
                     /translation="MIPGEYQIQDGEIELNAGRRTLRVTVANSGDRPIQVGSHYHFFE
                     TNDALSFDRAAARGMRLNIPAGTAVRFEPGQSREVELVDLAGHRRVFGFAGRVMGEL"
     gene            24232..25932
                     /gene="ureC"
                     /locus_tag="VFF22_12510"
     CDS             24232..25932
                     /gene="ureC"
                     /locus_tag="VFF22_12510"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011269033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HZX17666.1"
                     /translation="MKISRQAYADMFGPTVGDKVRLADTELWIEVEKDFTTYGEEVKF
                     GGGKVIRDGMGQGQLCAADVVDTLITNALIIDHWGIVKADVGLKDGRIAAIGKAGNPD
                     IQPDVTIAIGAGTEVIAGEGMILTAGGIDTHIHFICPQQIEEALMSGVTTMIGGGTGP
                     ATGTNATTCTSGPWHMARMLQAADAFPMNLGFTGKGNASLPEPLIEQVKAGAIGLKLH
                     EDWGTTPAAIDNCLSVADQFDVQVAIHTDTLNESGFVETTLAAFKGRTIHTYHTEGAG
                     GGHAPDIIKACGFPNVLPSSTNPTRPFTRNTIDEHLDMLMVCHHLDPSIAEDVAFAES
                     RIRRETIAAEDILHDLGAFSMISSDSQAMGRVGEVITRTWQTADKMHKQRGPLEGDGP
                     GNSNFRVKRYIAKYTINPAITHGISHEVGSIEVGKWADLVLWRPAFFGVKPTLILKGG
                     AIAASLMGDANASIPTPQPVHYRPMFASYGGSRHATSITFISQAAFDAGVPEQLGLKK
                     KIGVVKGCREVQKTDLIHNDYLPTIDVDPQTYQVKADGQLLWCEPADVLPMAQRYFLF
                     "
     gene            26034..26498
                     /locus_tag="VFF22_12515"
     CDS             26034..26498
                     /locus_tag="VFF22_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008093797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17667.1"
                     /translation="MHAPSRYLLGALLLLGLPLAQAETLTPLQGQSSEQIQSDTAACQ
                     AQSGSSQQQPDSNSVGGERLRGAAKGAAVGATAAQIRGNQHDEIYDRVDDDVKQQYRQ
                     NRARDAAAVGVAVGGSRQRQDRRENRRNAESSADSQQAYINCMSGRGYSVVP"
     gene            complement(26597..27043)
                     /locus_tag="VFF22_12520"
     CDS             complement(26597..27043)
                     /locus_tag="VFF22_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004425170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HZX17668.1"
                     /translation="MTIFEALRISHDRQRELAEKMVATSGDSPEREALFAELKDELAA
                     HSVAEERHFYIPLMQDDLGVDLSRHAIAEHHEMDEMVEGLEETDLSSPSWLVQAKALS
                     EKVHHHLQEEEQKFFQMAGKLLSEAQKKQLSGAYLSTYDEQKIAAN"
     gene            complement(27119..27385)
                     /locus_tag="VFF22_12525"
     CDS             complement(27119..27385)
                     /locus_tag="VFF22_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HZX17669.1"
                     /translation="MAGGWANDGAVQEQIDSSIEDAIQRARRQLPRGESLGHCEECDA
                     AIPEARRKAIPGVRLCVACQTKHDKEQAKFNGYNRRGSKDSQLR"
     gene            complement(27459..28322)
                     /locus_tag="VFF22_12530"
     CDS             complement(27459..28322)
                     /locus_tag="VFF22_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HZX17670.1"
                     /translation="MQRASHHDLRAQFRALLAANSCYHTASVFDPMAARIAADLGFEV
                     GILGGSVASLQVLAAPDFALITLSEFVEQATRIGRVSRLPVIADADHGYGNALNVMRT
                     VVELERAGIAALTIEDTLLPAQFGRKSTDLISIDEGVGKIRAALEARVDSELAIIART
                     HAGVLEVSEVIRRTQAYQAAGADAICLVGVEDFAHLEQIAAALSIPLMLVTYGNPKLR
                     DNARLASLGVRIVVNGHAAYFAAVKATYDCLREQRGINAGDLSATELTHTYTQPEDYI
                     VWARKFMQVKE"
     gene            complement(28429..29427)
                     /locus_tag="VFF22_12535"
     CDS             complement(28429..29427)
                     /locus_tag="VFF22_12535"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZX17671.1"
                     /translation="MPTSRPALLDLAARAQLFGHLAAMEKAGVPVERALLSAQVPVRA
                     RAALANQQTQIAGGGDMASAGLRSGLFTQLDSTLLHAAQVSGCLEAVYQRLADRYNHR
                     AQQVAAVKSRLLMPAAVLVLALFIQPLPALVGGQLSIAGYLWGVLAPLLVLGALYGLG
                     RGLYLRREHAPAGAASPLDGLLASLPIVGPALLRRNLRDFFDSLGLMLEAGMPMLEAL
                     PKACAVISHAPLRRRFSSVKVAIQRGQGLAQALASVDFPGQGLALGLIRTGEASGTLP
                     ASLLNYAGVETQKLDSLTEQLATWLPRLLYAGVMLWMAYGLLTGAGFGPRLPVELQ"
     gene            complement(29502..30257)
                     /locus_tag="VFF22_12540"
     CDS             complement(29502..30257)
                     /locus_tag="VFF22_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX17672.1"
                     /translation="MLKPCCALLLLLLACLAGPALADLHLLTEDAPPTSFSRDGELHG
                     LAVEVVRALIERTGDSGEIELLPWTRAYHLAQLEPDTAIFSTVRTPEREAKFQWVGPL
                     LIGTTSFYSLKSRNLHFDSLQQAAESGPLAVPKQWYTFETLNARGFKNIYGVTSSKNM
                     VTMLKHERVQLIATEDLTLKDELATGGLRPEQVQAHLPFMRSAYYIAFSLQTDAALVE
                     RWRRALDEMHQDGSFAQIFRRWLPHAELPTTTE"
     gene            complement(30311..31078)
                     /locus_tag="VFF22_12545"
     CDS             complement(30311..31078)
                     /locus_tag="VFF22_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX17673.1"
                     /translation="MRLMSWLGVVLLGCSASVQAAELRLYTEEYRPLSYIEDGKLTGM
                     AVEVVELLIQRTGQDTRIELVPWTRAYHQVQREANSGLFSTVRTARREALFQWVGPIA
                     LGHTSFYARRGAGLNVRTLKDVERFNTLAVPKQWYSYEYLSDRGLKNLYGVPTPQHMM
                     KMFKHGRIELLVANNLALDDMLAQQGMRRDEVELQFTLMGNNSYIAFSKSTDPALVQQ
                     WQQALEELLRDGSLERIHQRWFAQPFAMPASGEPLAR"
     gene            complement(31169..32122)
                     /locus_tag="VFF22_12550"
     CDS             complement(31169..32122)
                     /locus_tag="VFF22_12550"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZX17674.1"
                     /translation="MYRRCPSKYVLLFVVLTSWVAQALAKDCIGVVAAGATPFWGQVE
                     AGARQAGQQYALDVHFRGPLHEGRVETQLQMINWVVEHGCKALVIAPSGSEIVPRVQQ
                     LADHGINTVYFDRDLPGSAVRGVVATDNFGAGLQAGQALARALGGQGEVALLRLKAGV
                     PSTSERERGFRQGAEQGGLSILVDAYIGDDSQAAVDVLRDRLSQLAGVFTPNSTSSRA
                     ALAALRRLGKAGHLLHIGFDADPLLIEALSKGEIHSLMTQQAHAIGYQAVQLAAKSLR
                     GELPLQPVNVPLQARQVSRDNLAQWQRAQEVELSGNPSPSR"
     gene            32373..33101
                     /locus_tag="VFF22_12555"
     CDS             32373..33101
                     /locus_tag="VFF22_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HZX17675.1"
                     /translation="MTDTTQSTQATHKPRPLIDLLVSILIPSLILMKLSGESRLGADG
                     ALMLALAFPLGWGSFELLKYRKFNFIALLGLISVLLTGGIGLLKLDNQWLAIKEAAIP
                     GIIGIAVLVSTRTRYPLIRTMLFNKTVLNVDKIHERLEQAGNTELFETRLLRATYWLS
                     GTFFFSSAMNYVLAKWIVISPAGSEAFNAELGRMNLLSYPMIAIPSMIMLMAIFYYLW
                     RTIHGLTGLKLEDVMANAEQEKQP"
     gene            33116..33553
                     /locus_tag="VFF22_12560"
     CDS             33116..33553
                     /locus_tag="VFF22_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003302457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HZX17676.1"
                     /translation="MLTLSAYLGLFISALGAATLLPLQSESVLVALLLSGAYSPWALV
                     AVASVGNILGSLLNWLLGRYLEHFRHKPWFPVTDARLQQAQRGYARYGRWSLLLSWVP
                     IIGDPLTLVAGVMRERLWVFLLIVSLAKTARYAVLAALTLGWS"
     gene            33698..34654
                     /locus_tag="VFF22_12565"
     CDS             33698..34654
                     /locus_tag="VFF22_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HZX17677.1"
                     /translation="MMKRSDLIWTLVGLGAVLLSCYLLYHQVRTISLDEIADSLRAIP
                     RLDWLLAAAATLGAYSALAWYDRIALAHLGKKIPWRFISLCSFTTYALAHNIGASVFS
                     GALVRYRAYRSKGMTPQEIGILIVFCSFTFVLGTILASGCVLVLQPDLIHRVAKVTPL
                     VSEIIGGLLLLLVALYVLGSWRHFKPWHWGKFHLEYPRLGIVGRQLLAGPLELACAAA
                     IIYFALPAENNPGYLVVFGVFLASFSLALLSHAPGGLGVLEVTFLAAMPELPAADVLA
                     ALIVFRCFYLLLPFALSLLVVLGFEWTQWQSRRTPPNTPPQP"
     gene            34815..36080
                     /locus_tag="VFF22_12570"
     CDS             34815..36080
                     /locus_tag="VFF22_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZX17678.1"
                     /translation="MTFSTPARACGIDFGTSNSTVGWLRPDAETLIPLEDGKITLPSV
                     VFFNLEERRPVYGRLALQEYLEGYEGRLMRSLKSLLGSKLLKSETTVLGSALPFKDLL
                     GFFIGELKNRAEATAGRPFEEVVLGRPVFFVDDDPVADLEAQNTLVAVAHKLGFKDVS
                     FQYEPIAAAFDYESGIEREELVLIVDIGGGTSDFSLVRLAPERRTVADRHADILATGG
                     VHIGGTDFDKQLSLAGVMPLFGYGSRMKSDAPMPTSTHLNLATWHTINAVYSAQSQRQ
                     LQSMRYDIVDPTGIDRLFQLIERRDGHWLAMQVEESKIALTEQDARPIDLSRLEDGLV
                     AELTRELFEGAIEPLLERVRASVTQLLSDASVSVAQVDTVFFTGGSSGIPALRQSVAA
                     MLPNARHVEGNTFGSIGSGLAIEAKKRYG"
     gene            complement(36141..38456)
                     /locus_tag="VFF22_12575"
     CDS             complement(36141..38456)
                     /locus_tag="VFF22_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017684186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HZX17679.1"
                     /translation="MQTAKPLFDYPKYWAECFGPAPFLPMSREEMDQLGWDSCDIIIV
                     TGDAYVDHPSFGMAIIGRLLESQGFRVGIIAQPNWQSKDDFMKLGEPNLFFGVAAGNM
                     DSMINRYTADKKIRSDDAYTPGGMAGKRPDRASLVYSQRCKEAYKNVPIVLGGIEASL
                     RRIAHYDYWQDRVRNSILIDASADILLYGNAERAIVEVAQRLSYGHKIEDITDVRGTA
                     FIRRDTPQGWYEVDSTRIDRPGKVDKIINPYVNTQDTQACAIEQEKGPQEDPNEPKVV
                     QILASPRMTRDKTVIRLPSMEKVRNDPVLYAHANRVLHLETNPGNARALVQKHGEVDV
                     WFNPPPIPMTTEEMDYVFGMPYARVPHPAYGKEKIPAYDMIRFSVNIMRGCFGGCTFC
                     SITEHEGRIIQNRSEESIIREIEEIRDKVPGFTGVISDLGGPTANMYRIACKSPEIES
                     ACRKPSCVFPGICPNLNTDHSSLIQLYRSARALPGVKKILIASGLRYDLAVESPEYVK
                     ELVTHHVGGYLKIAPEHTEEGPLNQMMKPGIGSYDKFKRMFEKYSKEAGKEQYLIPYF
                     IAAHPGTTDEDMMNLALWLKGNGFRADQVQAFYPSPMATATAMYHSGKNPLRKVSYKS
                     DGVTIVKSEEQRRLHKAFLRYHDPKGWPMLREALTRMGRADLIGPGKNQLIPLHQPAT
                     DSYQSARRKNSTPAGSHKVAKDTTKSKPMLTQHTGLPPRDTGAAGGNPWDKREQAKAA
                     AQVRNQQAAKERADAAKGKGKKPVKKPAVPR"
     gene            38884..39708
                     /locus_tag="VFF22_12580"
     CDS             38884..39708
                     /locus_tag="VFF22_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17680.1"
                     /translation="MLTPAEKLSSRYVPLQRLSPADTQQMYAVFSQYYQNTTQQTFLN
                     DLSRKSGVILARTRKSKRIVGFSTLTDFEITVDGKPARGLFSGDTIVEPAYWGARCLH
                     VAFYLQLMRVRMRYPLTPLYWLLISKGYKTYLLLTRNFYAYFPDRNTPNSPLQQVVAA
                     YSQQLFPDAFDPQRMLLDFGEGAQHLKDEVAGITPEMCAQDADIAYFEQCNPSWRRGT
                     ELPCAGEVSYSALLRYCSRLGLKLLRRNLNWRAAATAAQNQAQANTARDGATELSQ"
     gene            39705..41336
                     /locus_tag="VFF22_12585"
     CDS             39705..41336
                     /locus_tag="VFF22_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HZX17681.1"
                     /translation="MTAVIASRCGHGLLRAFMHSGMQRFERQAHDLEAVQRRLLRDWS
                     QRFVRSPRGRNSGIRPEDSWEQVTACLPSSHYSDYQDDLERQRRENNQRLIDSPISRY
                     QPTSGSTSAIKWIPYTRTFLAELDRAISPWIGDLYRQYPQIAQGSHYWSLSWIPSALR
                     DTSSADLNDDMQLMSLGKRLIAGLTQTAPPGIALAGSADDSLFATLSFLAADARLSVM
                     SIWSPTFGLGLLEQLGHCREELAEVLSSGHWGRRSAAMAALRCPRSTAAAQRLRAWDG
                     NADPAFFAELWPQLALVSAWDTAAAAPWAQRLQQLLPQARFQGKGLWATEGVVTLPFR
                     GRYPLAYRSHVYEFEDLDNGRILPPWALRQGQQVMPLLTTGSGFARYRMNDVLEVSGH
                     WRTLPCFRFLGRNDGVDLVGEKISATLAQTVLDRLDYAGRLPITLLALDDSSGGRPGY
                     VLLLESSAEQSLPALQSLAQQLENALLDNFHYRLARDLGQLAPARCIAQPQMRQLYLE
                     QARARGMLEGNIKLEALRHWPGELPASLRHLLQVS"
     gene            41336..42442
                     /locus_tag="VFF22_12590"
     CDS             41336..42442
                     /locus_tag="VFF22_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17682.1"
                     /translation="MPDTHEILVRPARPEDNSALLDLLRRTPQMGSVTLTFEREPDFF
                     SGARVACEVPRVLVAERREHPGRLLGMVDIGQRQQYLDGQPCTVSYLHDLRIAAEGRG
                     GRVLAALFRAIATAMQAPGSWMEAVILEDNLVPLGIVGRGRAGLPNFYPNGRITTSLL
                     PGRQSRSQADSGLQIRAASAADAQQLQQLWQQAGSRQGFPVYQVQDLLEGSPYYQGLQ
                     IEDYWLACIDQRVVGVVGVWNQKAFKQTRVARYSPWLAALRRPYNLYSQLFGGLHLPA
                     VGNTFNYLNLHGLSVLNNDPLIFQALLEHVLAHQVQAQQSLVCGFFDDDPLAQVVRPY
                     RRQTLHSCHFLLSYDHDPRPAQARGPRHVEIARL"
     gene            42420..43550
                     /locus_tag="VFF22_12595"
     CDS             42420..43550
                     /locus_tag="VFF22_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003104593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HZX17683.1"
                     /translation="MSKSPACENRPTMTHAEPARPLATTPVDLDPGYAMDAKTLRRLS
                     LFAAVLLCIALAFGAYWLLHARFLESTDNAYVRADIVVVSSQLAARITRVQVEDNQWV
                     EAGDTLARLDDADFVYQEQRIQTQIDDLQVQLHSLTNQHAQQQARIEAQAAEVQASSA
                     ELGRQQGNLERIRALRRGGFASEQQFSSLGANVAVARASVDKANAGLKGQRLELSQLD
                     NRRQQLQNQLDGARNDLAQARLNLARTQIVAPIAGRVGNRSLREGQYVRPGEALLALV
                     PRSVWVQANFKETQVARMHPQQKVELRFDALPELRLQGTLDSLYPATGAEFSLLPPDN
                     ATGNFTKVIQRVPVKIRLPENIPELERIRPGMSVVATVDSRD"
     gene            43543..45087
                     /locus_tag="VFF22_12600"
     CDS             43543..45087
                     /locus_tag="VFF22_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZX17684.1"
                     /translation="MTEQPLAQREPSLRDWIAVLSAMLGAFMAVIDILITNASLKEIQ
                     AALGATLSEGSWISTSYLVAEIIIIPLTVWLVDLLGARRLTVWLTIGFIGSSLLCSMA
                     WSLHSMITFRALQGLCGGAFIPLAFSMVLTRLPPHRRPIGMALFAVTATFAPAIGPSI
                     GGWLTEHLGWQYLFYINLPPGLLMICGLLYGLEKRAIDWRQIRRADYAGICSMAIGLG
                     CLQVFLEEGYRSDWFSSRMIVVLALIAFVSLGCFVIIQLSREHPLLNLRILKNRNFAL
                     SCVAAVGLGIGLYGSIYALPLYLSQIQHYSPLQIGELIMWMGLPQLALIPFVPLLMRV
                     IDPRLLCAIGFMLFGYASFMSGSLNPDLSGPQLLHIQLIRAIGQPLILITSSLIATSY
                     IEHKDAGSASALFNILRNLSGAIFIALLMTMLQNDTSRNYQYLSESVSNLNPQALARL
                     EWLTEKLGSSQRALAVLSQETREQASILAYNEAFHIMGCALVICVLAMLFTRRLPPGL
                     KPGEVH"
     gene            45257..47131
                     /locus_tag="VFF22_12605"
     CDS             45257..47131
                     /locus_tag="VFF22_12605"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZX17685.1"
                     /translation="MLLGLAAGVCAAPALLKDDSSGSSLNEHIELLEDVGGQMSIADM
                     ADPAVQSRFTPANGRASVGQSRNPWWIKLTLQRSADAPSQWWLEVSSVTQFDLRLYLP
                     DQNGNWQERQSGELVSHKEGRDHEFRHMLFKLPELDQEPLTLYLRSYDPAGNSFPMRA
                     WQLADLTQQAAEKNLALGVVYGIIAALLLYNLFIFFSLRDSAYFWYVLTTAGALLMIL
                     SMSGHGFQYLWPNNPVPFWLDRVTLPSLWGFCACRFTQCLMQTRTHVRWAHHLLSLAC
                     VCYVIAVALEGLGQRHTAAWIIALLSLTSIPAALGSALIRWRQGYFPALLYLCGYGLI
                     LGSISLALMRATGLVQPATWNAYVFPLSVAAESILFSFALAYRIQTLKQEKAEALVQA
                     DREKTARLAQMQGSADELQNAVKLRTAELALANQQLSERERELQHAAFHDPLTELPNR
                     RYLVERCEAALAEAKRHNQAVALLLIDLDHFKPINDRHGHDAGDQMLQAIAQRLREHV
                     RAGDAVARLGGDEFAVLICGADAEQHAREIARRLLDELAKPVTYGAARLTVTISIGVA
                     LYPRHASNFTTLYKAADEMLYKVKSRGRSGSAVCGEDGELSSSARLQVDVLNVPSGLG
                     "
     gene            47251..49155
                     /locus_tag="VFF22_12610"
     CDS             47251..49155
                     /locus_tag="VFF22_12610"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZX17686.1"
                     /translation="MQRLTLAILLFLSLFGTLACASPVLLSAPSSGSMLNGQIELLED
                     VGGQLSIADMADPAVQSRFQPAAGRTSVGQSSNPWWIKLSLQRAIDAPAQWWLEVGAV
                     TLLDLQLFLPDDQGGWQTRQAGERVSFAEGRDHDYRRAVFSLPPLGEQPQTVYLRTYD
                     PAGNSFPLRIWQLHDLEQLAKREHLILGLIYGVILALLLYNLFILLALRDPAYLWYVL
                     TMAASLVFIASMSGHGFQYLWPDGPVPTWLDRITLPILVSLGILRFAQELLSTRTTLR
                     IAHHILNGCIAVYLLALAANLAGYRSETGLLIGLIPIVTVPTLLLSAVIRWKQGFTPA
                     LFYLLGYGSVLLSFVILVLRAAGVVQPGESTAYLFPLAVAAEAILFSFALAYRIQILK
                     QEKADAILQADREKTARLALAQANAADLQRSVELRTAELAATNERLRQREQALQHAAF
                     HDSLTELPNRRYLVERCEAALANARRHSETIALLLIDLDHFKPINDKHGHDAGDLMLQ
                     QIALRLREHVRAGDAVARLGGDEFAVLVCGADAEEHAREIANRLLAELAKPVMYRAER
                     LTVTISIGVALYPQHAGTFTALYKAADEMLYRVKARGRSGSAVCGEDGKLSDSTRLHL
                     DVLSVPSGLL"
     gene            complement(49157..50236)
                     /gene="alr"
                     /locus_tag="VFF22_12615"
     CDS             complement(49157..50236)
                     /gene="alr"
                     /locus_tag="VFF22_12615"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017518271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZX17687.1"
                     /translation="MRPLVATIDLSAIAHNYAVAKRCAPGRQAFAVVKANAYGHGVRE
                     VVTALHDVADGFAVASLEEAAEVRALHGEARILLLEGCFEADEYQFAAQLGLDVVLHS
                     EQQAQQLLAAGLTRPLNVWLKLDSGMHRLGFSSAAVREWHGRLQGVAQVAELNLISHF
                     ACADERGSEVTELQLEQFLDLLDLDFAQRSLANSAAILTIPAAHMDWLRPGIMLYGAT
                     PCADLGVAELGLRPAMSLTAQLIACRAVAVGETVGYGGNWRAERPSRIGTVSCGYADG
                     YPRHAPAGTPVVIQGQRVALVGRVSMDMLTVDLTDLPAAAVGDAVELWGSQLPVDEVA
                     QAAGTIGYELLSKVTARVPRRYRSS"
     gene            50487..50852
                     /locus_tag="VFF22_12620"
     CDS             50487..50852
                     /locus_tag="VFF22_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZX17688.1"
                     /translation="MNAINWFELFVSNFERARGFYERALATPLEVMEGMGGRMALFPH
                     AQESGVGGCLSESAEQQSGIGGTRVYLNVEGQLDAVVNRVPSAGGVIIHSKMSIAPHG
                     FIAVIKDSEGNEVGLHSMS"
     gene            50916..51617
                     /locus_tag="VFF22_12625"
     CDS             50916..51617
                     /locus_tag="VFF22_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HZX17689.1"
                     /translation="MRRADRLFQIVQFLRSRRLTTAQWLAERLQVSVRTIYRDIQDLS
                     LSGVPLEGEAGVGYVLRQPMDLPPLMFEREEIEALLVGARMVKAWADPQLQRAAESAL
                     AKIHSVLPNELRDELNRNQLFAPETNPYPVHWLGQLRQAIRQRHKLLISYRDERGDTS
                     QRSVRPLGLFFWGQTWTLCSWCELRNDFRNFRIDRVEQLQDRAETFEAVEGQRLEDYL
                     KPYLGEHATAPLYKS"
     gene            complement(51648..53042)
                     /gene="dnaB"
                     /locus_tag="VFF22_12630"
     CDS             complement(51648..53042)
                     /gene="dnaB"
                     /locus_tag="VFF22_12630"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HZX17690.1"
                     /translation="MTDISLPEQYDLQTSALKVPPHSIEAEQAVLGGLMLDNNAWERV
                     LDAVSDGDFYRHDHRLIFRAIFKLAERNHPFDVVTVSEQLDQEGQLSQVGGLAYLGEL
                     AKNTPSVANIKAYAQIIRERATLRQLIGISGEIADSAYMPQGRTGAEILDEAERLIFQ
                     IAEARPKTGGPVGINDILVKAIDRIDTLFNAGDAITGLSTGFTDLDDLTSGLQPADLI
                     IVAGRPSMGKTTFAMNLVENALMRSDKVVMVYSLEMPSDSIVMRMLASLGRIDQTKVR
                     AGRLDDDDWPRLTSAVNLLNDRKLFIDDTAGISPSEMRARTRRLAREHGEIGLIMVDY
                     LQLMQIPGSSGDSRVNEISEISRSLKALAKEFNCPVIALSQLNRGLEQRPNKRPINSD
                     LRESGAIEQDADIIMFVYRDEVYHPETEYKGVAEIIIGKQRNGPLGTARLAFLGKFSR
                     FENLAPGSYQFDDE"
     gene            complement(53162..53608)
                     /gene="rplI"
                     /locus_tag="VFF22_12635"
     CDS             complement(53162..53608)
                     /gene="rplI"
                     /locus_tag="VFF22_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HZX17691.1"
                     /translation="MELILLEKIANLGNLGDKVNVKAGYGRNFLLPQGKATAATAANV
                     AAFEARRAELEKLAAEKKAYAESRAAQLAELEVTITATAGDEGKLFGSIGTHDIAEAL
                     TASGVEVAKAEVRLPNGTIRQLGEYDVAVHLHSDVEATVRVVVVAA"
     gene            complement(53630..>54374)
                     /locus_tag="VFF22_12640"
     CDS             complement(53630..>54374)
                     /locus_tag="VFF22_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020306867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17692.1"
                     /translation="ILAWALLPAIGWWYFGEPRTLLVLLGALGLALLLRANLSWNRVL
                     LCSVALGLVYGLVLGAVFREPIESMALELQKLMPQMLDGVHQQMSVDERARLDSLIAP
                     VLTGLIAALLQIVSLLSLMLGRFWQAQLYNPGGFGREFRALRLPTPLAMLLLVGMLLG
                     PNLGPQMAMLTPLCSVPLAFAGIALLHGMVAQGRLGKFWLVGLYITLLLFMQLTYPLL
                     VVLAIVDSLLDFRGRFERKQGSGPANGEG"
BASE COUNT        10643 a        17884 c        15935 g         9912 t
ORIGIN      
        1 cgaaggtgtc gactttatcc tgatcaatcc tgccgctttt actcacacca gcgtcgcatt
       61 acgtgacgca ttgctggcag tgagcatccc attcatcgaa gtgcatttgt ccaacgtgca
      121 caaacgcgaa gctttccgcc atcactccta cttttcagat gtagctgtag gggtgatctg
      181 cggccttggc gccagcggtt accggctggc cctggaggct gcactcgaac aacttgaact
      241 taagcgcccc tgacctcacc gggagttgaa aaactatgga tattcgtaaa gtcaaaaaac
      301 tgatcgagct gctggaagaa tccggcatcg acgagctgga gattcgcgaa ggcgaagaat
      361 ccgtgcggat cagccgccac agcaagcaac ctgcttttat gcaacagccg atgtacgccc
      421 aggcacctgc gcctgtagct gctccggttg ccgcagctcc ggtcgcagct gaagccgccg
      481 cacctgctgc tgccaagctg aatggcaccg tggtgcgttc gccgatggtc ggcaccttct
      541 accgcgcatc cggcccgacg tcgggcaact tcgtcgaagt cggtcagagc gtgaagaaag
      601 gcgacattct ctgcatcgtg gaagcgatga agatgatgaa ccacatcgag gccgaaacca
      661 gcggcgtgat cgaatccatc ctgggcgaga acggtcagcc ggtggaatac gaccagcctc
      721 tgttcaccat cgtttgatcc acggagagcc agcgatgttg gaaaaagttc tgatcgccaa
      781 ccgcggcgag atcgccctgc gcatcttgcg cgcctgtaaa gagctgggca tcaagaccgt
      841 cgcggtgcac tccaccgccg accgtgaact gatgcacctg ggcctggctg acgagtcggt
      901 gtgcatcggc cccgccactg gcgcgctgtc ctatttgaat attccggcga tcatcagcgc
      961 tgctgaagtc accggcgcca ccgctattca tccgggctac ggctttttgg cggaaaacgc
     1021 cgacttcgcc gagcaggtgg aaaactccgg ctttgccttt gtcggcccga aagccgagac
     1081 cattcgcctg atgggcgaca aggtctcggc caagcacgcc atgatcaaag ccggcgtccc
     1141 tgtggtgcct ggctccgacg gcccgctgcc ggaagacgag gaaactgccc tgcagattgc
     1201 tcgcgaagtc ggctacccgg tgatcatcaa ggccgcaggc ggcggcggtg gtcgcggcat
     1261 gcgcgtggtg tacaaggaag aagacctgat caaatcggcc aagctgaccc gcagcgaagc
     1321 cggttcggtg ttcggcaacc cgatggtcta tttggagaag ttcctcggca acccacgtca
     1381 cgtggaagtc caggtactct ccgacggcca gggtaacgcc atccacctgg gtgaccgcga
     1441 ctgctcactg cagcgccgcc accaaaaggt actggaagaa gcgccggcac cgggcatcga
     1501 tgaaaaagcc cgcgccgaag tgctgcaacg ctgcgtcgat gcctgcatcg agatcggcta
     1561 ccgcggcgct ggcaccttcg agttcctcta cgaagatggt catttctact ttatcgagat
     1621 gaacacccgc gtacaggttg agcacccggt ttcggaaatg gtcaccggca tcgacatcgt
     1681 caaggagatg ctcagcatcg ccgctggcaa caagctgtcg tttacccagg aagacgtagt
     1741 gatccgcggc cattccctgg aatgccgaat caacgccgaa gacccggaca acttcatgcc
     1801 ctgccccggc aaggtcaagc acttccatgc cccgggcggt aatggcgtac gggtcgattc
     1861 gcacctatac agcggctaca ccgtaccgcc gcactacgac tcactgatcg gcaagctgat
     1921 cacctacggc gccacccgcg acgaggctct ggcccgtatg cgcaacgccc tggacgagat
     1981 cgtggtcgac ggcatcaaga ccaacgtgcc gctgcaccgc atgctggtcc gcgacaaagg
     2041 cttctgcaaa ggcggcgtga atatccatta tctggaaaag aaactgggta tggataagca
     2101 ctaagcttcg gcgtagcgca cagacaaggg ctgccattcg gcagcccttg tcgtttccgg
     2161 cctgccagcg gtggcagccg ccccatcgct tcaagtaagc tgcgcgccaa tttgcgccat
     2221 cccgccacaa cgttcatgag gtttcccatg ccctggttac aagtccgtct cgccatcact
     2281 ccggagcagg cggaaaccta cgaagacgca ctgctggaag tcggcgccgt ctcggtgacc
     2341 tttatggacg ctgaagacca gccaattttc gagccggacc tgggcaccac gccgttgtgg
     2401 tcgcacaccc acctgctcgc tctgttcgaa gccgataccg acgcgggcgc ggtatttgct
     2461 catctgcaac tgctgaccgg taccgacttg ccggaacatc aggccgaagt gatcgccgat
     2521 caggactggg aacgcagctg gatggacggc ttccacccaa tgcgtttcgg tcagcgcctg
     2581 tggatcgtgc cgagctggca cgcagcgccg gagccggacg cagtcaacct gctgctcgat
     2641 ccgggcctgg ctttcggcac cggcacccac ccgactaccg cactgtgcct ggaatggctg
     2701 gacggccagc cgctgcagga ctgcagcgtg atcgactttg gctgtggctc gggcatcctg
     2761 gcgattgccg ccctgctgct cggcgcgccg caggcagtcg gcactgatat cgatattcag
     2821 gccatcgagg cctcgcgcga taacgctggg cgcaacggta tcgacccggc gcgtttcccg
     2881 ctttacctgc cggacgacat gccgcaacaa ccggccgacg tggtggtcgc caacatcctc
     2941 gctggcccac tggtggcgct ggccccgcag atcaccagct tggtcaagcc gggcgggcgc
     3001 ctggcattgt cgggcattct cgccgaacag gccgacgagg tgcgcgcagc ctacaacgac
     3061 gcctttatcc tcgacccgac cgcagataaa gacggctggg tgcgtattag cggcgtacgc
     3121 cgctaaccgc cccagcgcgc acagctgctg cattaccaag cacttgcgtt agactagccg
     3181 ccttgtttag ccggatcgcc gcatgaccca gagcttcgtc acccagtgcc cccattgccg
     3241 caccagcttt cgcgtgaacc tcacgcagct gggtgccgcc catggtgccg tgcgctgcgg
     3301 agcctgcctg catgtgttca atgccgcgca acagctgcgt gagcagggcc agcaattgcc
     3361 cggcccagcg acgccagcag ctgcaccaac gccggcccca cagccgagca aaccaccagt
     3421 tccgcccgcg ccacgcccgg caatgcccag cgccgcgcca gcaataccgg ccgcgcctgc
     3481 cagcagcaaa accggcgata cgctgtggat tcatgacgac ctggacctcg acagcctcga
     3541 cctggatgaa gagctggcca agctcgaagc ccaggaacag cagctgtcca aacagttcct
     3601 ggcgattgat agcgcaccga aatacaacga aagttttctg ccagccgaac ctattgagca
     3661 cgacccgcac gacgagcgct gggccgaagc cctgctgcag gacgaactga gcaagccagc
     3721 tactagcctg agcgtcaaac ctcagccgct aacgcctcca gtacgcccga cgccgccgcc
     3781 cgctccggta acgcccgagc caatcaacga gcccgatgac gagccgccgc tcgacctcaa
     3841 cgcccttgac cgtcgccacc ccgaaccgga aattgagcac attgaactgc atgccgagcg
     3901 cgagccgttt agcgcgctga cagacaaccc cgcggatgaa gagcccgccg tagcggcgcc
     3961 gaaaaagacc gcgcgcagcg agcctgaact gcgtgacgaa cacctgttcg agctggatga
     4021 cgagccactg caactggact ggcaacagcc gaagaaaccc tggggccgct ggatcggctg
     4081 gggcctacta aacctgctcg gcgctgcggc gcttgccggg cagtacgtga tgtatcactt
     4141 caatgaactg gcgcgccagg atcagtaccg accctggttc gaacagctgt gcccggcagt
     4201 cggctgccaa ctgccatcca aggtcgacat cacccaggtc aaaagcagca acctggtggt
     4261 gcgcagccac ccggaattca gcggtgccct ggtggtcgat gccatcctct acaaccgcgc
     4321 ggcgttctcc cagcccttcc cgctgctgga aatgcgcttt gccgacatca acggccagct
     4381 gctcgccagt cgccggttca aacccagcga atacctcgct ggcgagctgg ccggcaacgc
     4441 ggaaatgcca ccgcagacgc ctattcacat ctccctggac atcctcgacc caggcaccca
     4501 ggcggtgaac tacagcctga gcttccactc gccggaatag gctgcacgca gcgaattgct
     4561 tgagaatccg ccgcaaccaa acccttcgcg ataagcccag agctgttcag aatttgttca
     4621 aaacagcctt tctccggtca tccagagcgg gtatcatgcc aacccttttt cgcactctcc
     4681 tatttgttca acagcaggtc tcttgagcag ggaagcccta tgtcggcgct acgcatcggc
     4741 ccctacacat tgccaaattc gctgattctc gccccgatgg cgggtgtcac tgaccagccg
     4801 tttcgccagc tgtgcaagcg tatgggtgcg ggtttggtgg tatcagaaat ggtcaccagc
     4861 gatgtgcgcc tgtggaacac ccgcaagtcg agcctgcgca tgatccacag cggtgatccc
     4921 gagccacgct cggtacagat cgccggtggt gatcccgaga tgctcgccga agcggcgcga
     4981 cgcaacgtgg aaatgggcgc gcagattatc gatatcaata tgggctgccc ggccaagaag
     5041 gtctgcaaca aggccgccgg ctctgccctg ttgaaagacg aaacgctggt ccgcgagatt
     5101 cttcaggctg tggtcgccgc cgtggatgtg ccggtgaccc tgaagatccg caccggctgg
     5161 gaccgtgaga acaagaacgg cattgccgtg gcgaagatcg ccgaagacgc cggcattgtc
     5221 gcgctggccg tgcatggccg cacccgcgcc gacctgtata tgggcgaagc cgagtacgac
     5281 accatcgccg caatcaagca ggcggtgtcg attccggtgc tggccaacgg cgatatcgat
     5341 tcgccgcaga aggccaaagc cgtactggcc gccactggcg ctgatggtct gctgatcggt
     5401 cgcgcggctc agggccggcc gtggatattc cgtgaggtcg agcattacct gcgtaccggt
     5461 gaacaactcc cggcacccgg cctgcgcgaa gtggaacgca ttctgcttga acatctggct
     5521 gcactgcacg ccttctacgg cgatgtaatg ggcgtacgca ttgcccgcaa gcatgtcagt
     5581 tggtatctcg caaccttgcc gggcgccagg gagttccgcg cccacttcaa tcgtctggac
     5641 agtacggacg cgcagtgcgc caacgttcgc gagtttttca gcgaacgtca taacaatgga
     5701 gaaggggtgg ccgcatgacg atgttgactg agaccttaga gagtggaatg gctcccgtga
     5761 gtgacaacag cagtttgaag cagcatctca atacgccaag cgaagagggg caaaccctgc
     5821 gcggcagcgt tgagaaggcc ctgcacaatt atttcgccca tcttgagggc gcagacgtct
     5881 gtgatgtcta caacttggtg ctgaccgaag tggaagcgcc gctactggaa accgtgatga
     5941 actacgtcaa gggcaaccag accaaggcct ccgagctgct cggcctgaat cgcggcaccc
     6001 tgcgcaagaa gctcaagcaa tacgacctgc tgtaaccccg aactcccgaa aagggcggcc
     6061 tatcatgagc cgcctttttt gctgataact cctttgctga tacttccgct ctgatggact
     6121 ctgaaatgac cgaccagacc acccgcctcc ccgttcgccg tgccttgatc agtgtttccg
     6181 acaagaccga catcctcgag tttgcccgcg agctcgtcgc cctgaatgtg gaaatcctct
     6241 ccaccggcgg cacttacaag ctgctcaagg acaacggcat cgctgcagtg gaagtggccg
     6301 actacaccgg cttcccggaa atgatggacg gtcgcgtcaa gaccctgcac ccgaaaatcc
     6361 acggaggcat cctcggccgt cgctccatcg acggagcggt aatggacgag cacggcatca
     6421 agccaatcga cctggtcgcg gtcaatctct atcctttcgc cgccactgtc gccaagcccg
     6481 gctgtgacct ggccgacgcc atcgagaaca tcgacatcgg cggcacaacc atggtccgct
     6541 ccgcggcgaa gaaccacaaa gacgtggcca ttgtggtaaa cgctggcgac tacgctggca
     6601 tcgtcgagtc gctgaaagcc ggcggcctga gctacgccca gcgcttcgac ctggccctga
     6661 aagccttcga gcacaccgcc gcctacgacg gcatgatcgc caactacctg ggcaccatcg
     6721 accagagccg cgacaccctg tccaccgaag agcgcggcgc gttcccgcgc accttcaaca
     6781 gccagttcat caaggcccag gaaatgcgct acggcgagaa cccgcaccag agcgcggcgt
     6841 tctacgtgga aacgcagaag ggtgaagcca gcgtggccag cgccattcag ctgcaaggca
     6901 aggaactgtc gttcaacaac gtcgccgaca ccgacgccgc gctggaatgc gtgaagagct
     6961 tcgtcaagcc agcctgtgtg attgtcaagc acgccaaccc gtgcggcgta gctgtagccc
     7021 tggacagcga aggcggcatc cgccaggcct atgagctggc ctacgccacc gataccgagt
     7081 cggccttcgg cggcatcatc gcgttcaacc gcgagctgga cggcgccacc gctcaagcga
     7141 tcgtcgagcg ccagtttgtc gaagtgatca tcgcgccgaa aatcagcgcc gaagcccgag
     7201 ccatcgtcgc cgccaaagcc aacgtacgcc tgctggaatg cggcgaatgg ccggccgagc
     7261 gcagcgccgg ctgggacttc aaacgcgtca acggtggcct gctggtacag agccgcgaca
     7321 tcggcatgat caaggccgaa gacctgaaga tcgtcactaa gcgcgcaccg agcgagcagg
     7381 aaatccatga cctgatcttc gcctggaaag tggccaagtt tgttaaatcc aacgccatcg
     7441 tctatgccaa aggccgccag accatcggcg tcggcgccgg ccagatgagc cgcgtcaact
     7501 ccgcccgtat cgccgcgatc aaggccgaac acgccggcct gcaagtcgcc ggctcggtaa
     7561 tggcaagcga cgccttcttc ccgttccgtg acggcctgga caacgcagcg gccaatggca
     7621 tcactgcggt gatccagcca ggtggttcga tgcgcgatgc ggaagttatc gccgcagccg
     7681 atgaggccaa tatcgctatg gtcttcaccg gcatgcgtca ctttaggcac taaaatcggc
     7741 cgcactcgga caggcatctg cggcgttgtc cggccctccc cccatgctca ttgccagaag
     7801 gcaactccgc tgggtgaagg gccagacgcc ttgcatctac cagcccgatc gcgacctcga
     7861 aatttgtagg gtggataacg cttcgcttat ccaccaaacg gccatacgcc gacctccatt
     7921 gggagagaaa catgaacgta ttgatcatcg gcagcggcgg tcgtgaacac gccctggcct
     7981 ggaaggttgc gcaggacaag cgcgtcaaca aggtcttcat cgcccccggc aacgccggca
     8041 ccgccactga agccaagtgc gagaacgtgg ccatcgacgt actggccatc gagcagttgg
     8101 ccgatttcgc cgaaaagaac gtgcagctga ccatcgttgg cccggaagcg ccgctggtga
     8161 aaggcgtggt tgatctgttc cgcacgcgca agctggatat ctttggcccg accgctgcag
     8221 ccgcgcagct ggaaggctcc aaggccttta ccaaggactt cctcgcccgc cacgcgatcc
     8281 ctaccgccga ctaccagaac ttcactgagg tcgagccggc cctgacctac ctgcaaaagg
     8341 tcggcgcgcc aatcgtgatc aaggccgacg gcctggccgc cggtaaaggc gtgatcgtcg
     8401 ccatgactct gcaggaagcc gaagacgccg tgcgcgatat gctcgccggc aatgtctttg
     8461 gcgaagcggg ttcgcgggtg gtcattgaag agtttctgga tggggaagag gcttccttta
     8521 tcgttatggt cgacggcgaa aacgtgctgc cgatggccac cagccaggac cacaaacgcg
     8581 tcggcgatgc cgacactggc ccgaacaccg gcggcatggg cgcttactcg ccagccccag
     8641 tggtcaccgc cgacgtgcac cagcgggtga tggctgaagt gatctacccg accgtgcgcg
     8701 gcatggccag tgaaggcaac gtctacaccg gcttcctcta cgccgggctg atgatcgaca
     8761 aggcaggtag ccccaaggtc atcgaattca actgccgctt cggcgacccg gaaacccagc
     8821 cgatcatgtg ccgcctggaa tcttccctgg tgctgctgat cgaagctgca ttggccaagg
     8881 cgctggataa ggtcgaagcc acctgggacc cgcgcccaac tgtgggcgtg gtgatcgccg
     8941 ctggcggtta cccgggtgac tacgccaaag gtgatgtgat cgaaggcctg gatgcggccg
     9001 cgcaactgga aggcaaagtc ttccacgccg gtactgcact caaagacggc cagatcgtca
     9061 ccgccggcgg ccgcgtactc tgcgccaccg ccattggccg caccgttgag gaagctcagc
     9121 aacaggccta tcgcctggct gagaagatcc gctggagcgg cagcttctac cgcaacgata
     9181 tcggttaccg cgccattgcc cgcgaacgcg gggataagtg aaggtaaata aagggtaaaa
     9241 cacaacaaag gtggccaatt ggccaccttt gtcatattac cctcgcgaac agcttggcta
     9301 taatccgacc acttactttc gaagggactt cactgtgcgc cggctgagga ttgccaccta
     9361 cctgctactc agcacactgc tgatggcgct caccgtagcg tcagctcagg caggagcaca
     9421 ggcatcctgg tccagcctca ccgacccgag cgccgaactg caatttaatg atgtgcgcag
     9481 ccccgagcgc caggcgcagt tccgctcgac cgatctcagc cagctctaca cccccggcgg
     9541 caacagcgct ctgtggctgc atcaccgtat gccggctaac actgacgcac agatgttgcg
     9601 ggtgttcgcc ccctacctgg cgtatctcga cctgtatgtg ctgcaaggcg atacgctgat
     9661 cgaacaggcg cacaccggta gcaacctgcc attttccagt cgaccgctag ccagccgcga
     9721 tttcctccta cccctgccta aagccgaaca gccgctggat atctacctgc gcctggcctc
     9781 tgaacatgcg ctgcgcccca gcattaccct gcaaagtgcg caagtgatgg tcgccgatga
     9841 caatcgcccg ctgctgttcg gcctactact cggctgcttc ggcatgctgg tggcctataa
     9901 ccttgtgcgc tttgcctaca cccgctcagt cagcggccta tggctcgcgg ccactcaggt
     9961 ctgccagctg gtggcagtgg tcagcctgct cggcatcagc gcgccttggc tcagcgaatg
    10021 gcaaagcctg cagccgcaga tcgccaacct gtcgatgctg ctggccgcgc tctgcgccct
    10081 gcgttttacc gccagtttct tccataaggt ctgcccgagt acgccactga atcacctgct
    10141 caccgctgaa gtggtggtga tcagcctggt ctgcgtggct ctattggttg cgaccaatct
    10201 gcaattcaat caactggtct atctgctcaa cgccgtcgcc ggcctgagta ttctgctggt
    10261 agcgctaagc cactggcggc atggctacca acccgcgcgt ctgtttagcc tggcggtgtt
    10321 gctgttctgc gcagccttta tttgcgccct gccgatcctc ttcggttact gggcggtaca
    10381 gagcgagtgg atggcttacg gcctgctcac catcaccgcc gtcagcggct ttatcctcag
    10441 catggcgctg agcgagcgac agcgacgcat catgcaggac cagttcagta ccagccgcgc
    10501 cctcgccgcc agctccgccg aattgaaagc caaggcggaa tttctcgcca aaatcagcca
    10561 cgaaattcgc accccgatga atggcgtgct gggcatgacc gaactgctgc tcggcacccc
    10621 gctctccgcc aagcaacgcg actacgtaca gaccatccac agctcgggca acgagctgct
    10681 caccctgatc aacgaaatcc tcgatatttc caagctcgaa tccgggcaga ttgagctgga
    10741 tgacgtgcag ttcgacctta acgcgctgat cgaagactgc ctggatatct tccgcgccaa
    10801 ggccgagcag cagaaggtcg agctgatcag ttttatgcag ccgcaagtgc cacgggtaat
    10861 cagcggcgac cccacacgcc tgcgccagac cctgctgagc ctgctggaca acgccttcaa
    10921 acagaccgac gaaggcgaaa tcctcctggt ggtcgctctc gacaccaata ccgaacaacc
    10981 acgcctgcgc attgccgtac aggacagcgg caaaccgctg gacgccagcg agcgcgacgc
    11041 actgctgaat gccgaactgc acagcaaaga cttcctcgcc gccaccaaac tcggcggccg
    11101 cctcggcctg atcatcgccc gtcagctggt gcgcctgatg gaaggcgaat tcggcattca
    11161 gagcggcggc agccagggct cgaccctgtg gctgaccctg ccgctggacg ccacccgcct
    11221 ggaacagccc accgccgacc tcgacgcctc gttgcaaggc gcgcgcctgc tggtggtgga
    11281 cgacaacgac acttgccgca aggtcctggt gcagcaatgc agcgcctggg gcatgcaggt
    11341 cagcgccgtg ccgtcgggca aggaagccat ggccctgctg cgcaccaagg cgcatcttcg
    11401 tgaatacttc gatgcggtgc tgctcgatca ggacatgccc ggcatgaccg gcatgcagct
    11461 ggccgcgcgg atcaaggaag acgccaacct caaccacgac attctggtga tcatgcttac
    11521 cggcatcagc aatgcgccga gcaagatcat cgcgcgcaac gccgggatca aacgcatcct
    11581 ggccaagccg gtggctggct acaccctgaa aaccaccctg gccgatgaac tggcgcagcg
    11641 cggcaagggt gattcgccga tgttcaaccc ggcgccacag agcacgccgc tgaacgtgcc
    11701 cactgatttc cgcatcctgg tcgccgaaga caacaccatt tccaccaagg tgatccgcgg
    11761 catgctcggc aagcttaatg tgcagcccga caccgccagc aacggcgaag aagccctcag
    11821 cgcgatgaag gcccagcagt acgatctggt gctgatggac tgcgaaatgc cggtgctcga
    11881 cggtttctcc gccaccgagc agctacgcgc ctgggaagcc gccgagcagc gcgtacgcac
    11941 tccggtggtg gcgctcaccg cgcatatcct caatgagcac aaggaacgcg cgcgccaggt
    12001 cggcatggac gggcacatgt ccaagccggt ggaaatgtcg cagttgcgcg agctgatcga
    12061 acactgggtc gccgaacggg aaatccgccg ccaacgcgac gccctgcctt cctgaggatg
    12121 cggccgggat tgcccggcag ccgctagact cttaccctgc cacccttctt tatcgagacg
    12181 agtcctttcc atggcgtccg cgctgttcag cctgtatttg aaactgctgg tgctctatag
    12241 ccccttcttc gtgctgtcgt gttttatcgg cctgagccgc ggctacacgg tcaaggagcg
    12301 caagcgcctg gcctggaaag tcgccctcgg cgtactgatc gccagcgtgc tgctgtacct
    12361 gttcggcaaa catatcttca ccctgttcgg catcaccatc gacgccttcc gcatcggtgc
    12421 cggcagcgtg ctgtttatct ccgctctggg catggcccag ggcaaatcgg cggtgcagag
    12481 cgataacgtg cagcaggacg tgaccatcgt gccgttgacc atcccgctta cggtcggccc
    12541 cggcaccatc ggtgcgctgc tgctgatggg tgccagccaa cccgactggg gcgataaggc
    12601 cgtggccgtg ctgggcatcg ccctggccag cctcacggtg ggcgtggtgc tgtatatgtc
    12661 caaccagttc gagcgcctgc tcggtgatca gggcttgcag atcgtcagcc gcctgatggg
    12721 gcttttcgtt tgcgccctgg ctgcgcagat catctttacc ggggtcaaga actacctcgc
    12781 cctctgactc cgcgcggatg cacttcgggc tgcaccataa gctggcaatc ggccccgccg
    12841 cggtgcacaa accgctctgc gccgccctct gaaaagccgc agcaagcggc taagccattg
    12901 aaatagatca acttggcaaa cctggcaccg ggcttgctct gaccacttca tcacttggat
    12961 acaagatgga attcagagca tgtccaaagc ccctatcgcc ttcaccctcg ccgactggca
    13021 acagacctac cgcgacggcc agtcgcccgc cgagctgctg catagcctgc gcctcgaact
    13081 gagcacagag gacaatgcct ggatcgccct gaccagcgac gctcagctca acgcccagct
    13141 cgatagcctg tccaccctgc ttgcaagctc cggcggcgag ctaagcaagt tgccgctata
    13201 cggcgtgccc ttcgccatca aggacaatat cgacgccgcc ggctggccta ccaccgccgc
    13261 ctgcccggag ttcgcctaca ccgctgcagc cgacgccacg gtggtggcca agctgcgtgc
    13321 ggccggcgcc attctgatcg gcaagaccaa cctcgaccag ttcgccaccg gcctggtcgg
    13381 cacccgctca ccccacggcg cggtgtgcaa cagctttaac ccggactacg tcagcggcgg
    13441 ctccagctcc ggctcggcca gcgtggtggc gcgcggcctg gtgcccttct cgctgggcac
    13501 cgacaccgcg ggttccggcc gtgtgcccgc tggcttcaac aatattgtcg ggctcaaacc
    13561 gagcaagggc tggctgtcca acaacggact ggtgccggcc tgcaagaccc ttgattgcat
    13621 ctcgatattt gccctgaccg tcagcgatgc cctgagcgtg gcgcagatcg ccggaggcta
    13681 tgacgcggcc gatccctaca gccgcaagaa cccgtgcagc gccaaggtcg gcatgccggc
    13741 ccagccgcgc ctggcagtac cgagcaaccc cgagttcttt ggcgacaccc agaaccaagc
    13801 agtgtttgag cgggccctgg gcaaactgcg cgagctggat gtgcagttgg tggaaatcga
    13861 ctttgcgccc ttccagcaac tggctgagca gctctattac ggcagctggg tcgctgaacg
    13921 caccgtggcg gtagaaggcg ttaatcccga ccatatcaac ccggtggtgc gcggcatcgt
    13981 caagaacggc cataaataca gcgcctgcga tgcctacaag gccgaatata tccgcgccga
    14041 actgagccgg aagatcaacg acgtgctggc cggcttcgat gccttggtgg taccaacctc
    14101 gccgaccatt cgcagcctgg ccgagatgga tgcagaaccg gtgctgttca acagccagtt
    14161 cggcacctat accaacttca ccaacctcgc cgacctgtcg gccctggcgg tacccgccgg
    14221 tatgcgtgcc gacgggctac cagccggtat caccctgatc gctccggcct ggcacgacca
    14281 agccctgtct gacttcggcc aacgctggca gcaggcgctc aacttgccgc tgggcgcaac
    14341 cggcaaagcg ctgcccgcac aagttgccag cagcagtcca gcccccggct gcgtgcgcgt
    14401 cgccgtagtg ggcgcgcacc tcaccggtat gccgctgaac ttccagctca ccagccgcga
    14461 cgccgtgctg gtggagcaaa ccagcagcgc cgccacatat cgcctgtacg ccttgcccgg
    14521 caccgtgccg ccgaaaccgg ggctggcgcg ggtggccgag gacggcgcgg cgatcatcgt
    14581 cgagctgtgg gatatccccc aggcgcgctt tggcgaattt gtcgcagaga ttcccgcgcc
    14641 gctgggcatc ggcaatctgc agctggccga cggccgctgg gtcaagggct ttatctgcga
    14701 gccgtatgcg ctcgctggcg cccgcgatat caccagcttc ggcggctggc gcgcgtttat
    14761 cgccagccag cagcagggct gaattcatgc agctgagcgg cacacccggc accggcaagg
    14821 agcgcccgga gaatctcgcc gagcgcatct accagcagct caaggacgac atcttcgagt
    14881 tccgcctgct gcccggtgac cgcttctccg aaggcgagat cgccgaacgc atggcggtca
    14941 gtcgtacgcc ggtgcgccag gcgctgtatc ggctacagcg cgaggattat ctgcaggtgt
    15001 atttccgcag cggctggcag gtcaagccgt tcgactttgc gcatttcgag gagctgtacg
    15061 aggtgcgcat cgtcctcgaa atggaagctg tgcggcgttt gtgtgaacgc ccccaagacg
    15121 agcactgcgc cgcactggag caactcaagc gcacctggtt ggtgcaaccc gagcagcgtt
    15181 tgcaggacgg ccgcgaggtt tcgcgcctgg atgagcgctt tcattgcctg ctagtggagg
    15241 ccaccggcaa ccgcgaaatg gcccgcctgc acgccgaggt cagcgagaaa atccgcatta
    15301 tcaggcgctt ggacttcacc cagggcccgc gcgtgacggc cacttacgag gaacacggac
    15361 aaattctcgc ggcaattctg tcgcgccgat gcgaagaggc acagctattg ctcaagaccc
    15421 atatagaggt cagcaaggcg gaagtgcgca agatcaccct gcacatgctg catagcgcgc
    15481 gtcagcgcac catgccggca ccagtggtga agacctgagg tgaagaccct agggggcgga
    15541 acctgtttta actgccagac aaacaactaa aaaccagcag ctcaaacact taagttcgtg
    15601 aatggagatc gcaccatgca acgtcgcaat ctgatcaagg cctttaccct atccgcttcc
    15661 atcgctgcca tgggcatgac ctggaccgtg caagccgccg agaccatcaa ggtcggcatc
    15721 ctgcactcgc tgtccggcac catggccatc tcggaaactt cgctgaaaga catggcgttg
    15781 atgaccatcg acgaaatcaa cgccaagggc ggcgtactcg gcaaacaact ggagccggta
    15841 gtggtcgatc ccgcctcgaa ctggccgctg ttcgccgaac gcggtcgcca gctgctgacc
    15901 caggacaagg tcgacgtcac cttcggctgc tggacctcgg tgtcgcgcaa atccgtgctg
    15961 ccggtctatg aagaactcaa cggcctgctg ttctacccgg tgcagtacga aggcgaagaa
    16021 atgtcgccga acgtcttcta caccggcgcc gcgcccaatc agcaagccat cccggcggtc
    16081 gagtacctga tgagcgaaga cggcggcgcc gccaaacgct acttcctgct cggcaccgac
    16141 tacgtctacc cgcgcaccac caacaagatc ctgcgcgcct tcctgatcag caagggcgta
    16201 gccgagaagg acatcgaaga ggtctacacc cccttcggtc atagcgacta tcaaaccatc
    16261 gtggccaaca tcaagaagtt ctccgctggc ggcaaaaccg cggtgatctc caccgtcaac
    16321 ggcgactcca acgtgccgtt ctacaaggaa ctggccaatc agggcatcga agccaccgac
    16381 attccggtag tagccttctc ggtcggcgaa gaagaactgc gcggcatcga caccaagccg
    16441 ctggttggcc aactggccgc ctggaactac ttccagtcgg tagagaaccc ggtcaacacc
    16501 aagtttgttg ccgactggaa agcctacgcc aaggccaaga acctgccgaa cgccgatacc
    16561 gtggtcacca atgacccgat ggaagccacc tacgtcggca tccacatgtg ggcccaggcc
    16621 gttgaaaagg ctggcaccac cgatgtggat aaagtgcgcg aggccatgta cggccagacc
    16681 ttcgccgcgc cgagcggtta caccctgacc atggacaaga gcaaccacca cctgcacaag
    16741 ccggtgatga tcggcgaagt ccaggaagac ggtcagttct ccgtggtctg ggaaaccagc
    16801 agcccggtcc gcgcccagcc gtggagcccg tatatcgaag gcaacgacaa gaagccggat
    16861 tacgcggtga agagcaactg atacctcggg gcctggcccc atctcccctg taggagccag
    16921 cttgctggcg atcgaagcga cgcggttgtc ctggtaaacc gcgatcgcga gcgagctcgc
    16981 tcctacagag tccatcttcc aggacgcact tatgcccact gcctttaccc gaattctgct
    17041 gagcctgctg ttgctgctgc ctatggccgc gcacgccggt gacgcccagg actttgtcgc
    17101 cgccagcccg gccaagcagg ccagcctgct ggaaagctgg tccgccgcgc cggatgccgc
    17161 gcgcctaccg ctgctcgaag ccctgcaaca aggccgcgtc gccgccgaca gcacaaagac
    17221 cgcctttatc gaagtaggtg gcgtttatca gccagccgaa ggcgaagctg tgcccactga
    17281 agcaccgcgc aaactgcgcc tgaataaccg actgcgcggg ctgatcagca ctgccgtggc
    17341 cagccaccag ctgctggccg aagacccggc gctgcgcctg gctgccgctc agcaattgca
    17401 gaagagtgcc aagccggcgc agctgcaact gctcaatgcc caggtcgcca gcgaaaccga
    17461 cgacgccgtg cgtgatgccc tgaccctggc cctggccaac ctgcagctgg tcgacagcga
    17521 cctggccgtg cgcctggccg ccgtgcgcct gctcggtgaa accggcgacc cgcttgcccg
    17581 cacccgcctg gaagcgctac tcgacccaag cgtggaaagt gacccgacag tgcgtaccgc
    17641 cgcggaaacc agcctggccc aggtcaaacg caaactgctg atcggagaga tccttggcca
    17701 ggcattcagc ggcctgtcgc tcggctcgat cctgctgttg gccgcgctgg gcctggccat
    17761 caccttcggc ctgctcggcg tgatcaatat ggcccacggc gagatgctga tgctcggcgc
    17821 ctattccacc tatatggtgc aggtgctgtt ccagcggttc gctccggaag ccctggcgct
    17881 gtatccgttg gtggcactgc cggtggcatt cttcgtcacc gctgccatcg gcatggcgct
    17941 ggaacgcacg gtgattcgcc acctctacgg tcgcccactg gaaaccctgc tcgctacctg
    18001 gggcatcagc ctgatcctga tccagctggt gcggatgatc ttcggcgcgc agaacgttga
    18061 agtggccaac ccctactggt tgtccggtgg catccaggtg ttgccgaacc tggtgctgcc
    18121 gtacaaccgc atcgtgatta tcggtttcgc cctgtttgtg gtgctgctga cctggctgct
    18181 gctgaacaag acccgcctcg gcctcaacgt ccgtgccgtc acccagaacc gcaatatggc
    18241 cgcctgctgc ggcgtgccga caggccgggt ggacatgatg gcgtttggtt tggggtctgg
    18301 gatcgctggc ctcggcggtg tggccctaag ccagatcggc aacgtcggcc ctgacctcgg
    18361 ccagagctat atcatcgact cgttcctggt ggtggtgctc ggcggcgtcg gccagctcgc
    18421 cggcagcgtg atggcggcct ttggcctggg catcgccaac aagattctcg aaccgcagat
    18481 cggtgccgta ctcggcaaga tccttatcct cgcgctgatc attctgttta ttcagaaacg
    18541 cccgcaaggt ctgtttgctt tgaaaggacg ggtgatcgat tgaaccagat tagccgcatt
    18601 gaagccgtag gagcaggcca tgcctgcgac cgcgttgccg ccattgttcg cgcgcatggc
    18661 gcgctcctac aaaccaacaa tcatttgctg cgacaggatg ggtgatcaac tgatgaccat
    18721 gccactcaac caaacgctat tggcgcgcgc cagcgccaag ctcggcccgc gagtttccat
    18781 cgctatcggc ctgctggtac tcaccctgct gatcgccatg ccgctgttgc acctgttgcc
    18841 ggcggacaac gccctgcacg tctcggccta taccctgacc ctggtcggca aaatcctctg
    18901 ctactgcatc gtcgccctgg cgctcgatct ggtctggggt tacgccggcc tgctgtcgct
    18961 cggccacggc ctgttcttcg ccctcggcgg ttacgccatg ggtatgtacc tgatgcgcga
    19021 agcagccggt gatggcctgc cagcctttat gagcttcctc gcctggaccg agctgccctg
    19081 gtattggtac ggcaccagca acttcctctg ggcgctatgt ctggtggtgc tggcccccgg
    19141 tttgctggcg ttggtgttcg gcttcttcgc cttccgctcg cggatcaagg gcgtgtactt
    19201 ctcgatcatg acccaggccc tgacgttcgc cggcatgctg ctgttcttcc gcaacgaaac
    19261 cggctttggc ggcaacaatg gtttcaccaa cttccgcacc attcttggtt tcgatatcac
    19321 ctcggcgcac acccgcgcgg tgctgtttct cgccaccgtg gtgctgttgg tcgccagcct
    19381 gtacctgggc tggaccctgg cacgcagcaa gttcggccgc gtgctcactg cgctacgtga
    19441 cgcagaaaac cgcctgatgt tctgcggcta cgacccgcgc ggctacaagc tgtttatctg
    19501 ggtgctcagc gcggtgctct gcggcctggc tggcgcgctc tatgtgccgc aggtcggcat
    19561 catcaacccc agcgagatgt cgccgaccaa ctccatcgaa gccgccgtgt gggtagccct
    19621 tggtgggcgc ggcacactga tcggcccgct actcggcgcc ggtttggtca acggcatgaa
    19681 gagctggttt accgtggcct tccccgagta ctggctgttc gccctgggct ttctgtttat
    19741 cgtcgtgacg ctgttcctgc ccaagggcgt gatcggcttg ttacgcaaga aaggagagca
    19801 atgatgcgcg ccactccggt ccccgaattt atgctcgaac ccgcctttga cccgtccggc
    19861 agtggccgtg atgcgatagg ggcaagcacc ctggccagca agggcctgaa cgttcgtcac
    19921 ggcactatcc tgaccctgga agatatcaac gtcagcttcg atggtttcaa ggcgctgacc
    19981 aacctgaccc tgtatatcgg tgtcggtgaa ctgcgctgca tcatcggccc caacggcgcc
    20041 ggcaagacca ccatgatgga cgtgatcacc ggcaagaccc gcccggacaa cggcgtcgcc
    20101 tacttcggtg aaaccctcga cctgacccaa atgagcgaag tcgagatcgc ccagtccggc
    20161 atcggtcgca agttccagaa gccgacggtg ttcgaagcgc tcagcgtgtt cgaaaacctc
    20221 gaactggcgc agaaaaccaa caagtcggtg tgggccagcc tgcgcgccaa actcagcggt
    20281 gagcagaaag accgcatcga tgaagtgctg cagacgatca agctcgacgc ctcgcgcaat
    20341 cgccctgccg gattgctctc gcacgggcag aagcagttcc tcgagatcgg catgctgctg
    20401 gtgcaggacc cgcaactgtt gctgctcgat gagccggtgg cgggcatgac cgacgccgaa
    20461 accgagttca ccgccgagct gttcaagtcc ctggcgcgca agcattcgct gatggtggtc
    20521 gagcacgata tgggctttgt cggttccatc gccgaccacg tcacggtgct gcaccagggc
    20581 agcgtgctgg ccgaaggctc gctggaacag gtgcaggctg atgagcgggt gattgaggtt
    20641 tatctggggc gctgatggtt ggtgcgcacg gcgcacccta cgtccgcggc cccacgcgta
    20701 gggtgcgccg cgcgcaccga aacgatcgcg acagcacaga acaacgaggt aaaaacatgc
    20761 tgcaagtcca acaactccac caatactacg gcggcagcca tatcctccgc ggcctgtcgt
    20821 tcgaggccaa ggtcggcgag gttacctgcc tgcttgggcg taacggcgtg ggcaaaacca
    20881 ccctgctgaa atgcctgatg ggcctgatcc cggccaagca aggcgcagtg agctgggaag
    20941 gccagccgat caccgcattc aagccgcacc agcgcgtgca cgccggcatc gcctacgtgc
    21001 cccagggccg ggaaatcttc ggccgcctga ccgtggaaga aaacctgttg atggggctgt
    21061 cacgcttccc cggcagcgaa gccaaggtgg tgccggaatt tatctacgaa ctgttcccgg
    21121 tgctgcgcga gatgaagcaa cgccgtggcg gcgacctctc cggcggccag cagcaacagc
    21181 tggccatcgg ccgcgcgctg gcgagcaagc cgcgcctgct tatcctcgac gaacccaccg
    21241 aaggcatcca gccctcggtg atcaaggaga tcggcgtggt gatcaagaag ctcgccgagc
    21301 gcggcgatat ggccattctg ctggtcgagc agttctatga ttttgccgcc gagctggccg
    21361 accagtacct ggtaatgagc cggggtgaga tcgtccagca gggccgtggc gaaaccatgg
    21421 aaaccgacgg tgtacgtggc ctggtcgcaa tctgaatggc gacgccgcac ctgcactttg
    21481 cgcataatca ggctttcgct tatggactcg aggccccaat gccacccctg cgcacgcttc
    21541 ttctcccgct taccgcactg gtcggtatgg cactggtgat ctgggccggc agcctgccgg
    21601 attactggat gctgcgcgcc atgccggttg gcagcgaagc ggtctatccg ctcaagccag
    21661 tgctgatttt ctgcgccatc gtcctggccg aatgcagcgt gctgttggcg gtgctgcgcc
    21721 cggcgtccta ttgccgctcc tggggccgcg ctttgtgtgc cagtgtgctg gccctgggcc
    21781 tagcgctgat ctggatccaa ggaattctgc atgccccgcc ctactacggc atgcacctgc
    21841 aatggtggct ggcggtcagc ctggggctga tcctgctgag cgcttattcc gcagttcagg
    21901 cctggcgaaa ccgcagcagc agggtgacgg cgtgaacgca ccggccgtct tgtttacccc
    21961 cagctggcat gccgagctgg agctgggcta tggccgcgat ggcgagacga cgcgaccgac
    22021 attgcgccgc cataaaggcc cgctgcgggt gcagaagcac ctgtatgccg aagggccaca
    22081 agtgtgtcag cacatcatcg tccacccccc cggcggcatc gccggcggtg atcgcctgga
    22141 tatctctgcc agcgttggca gcggtgcgtg ggcacagttg accagccccg gcgcggccaa
    22201 gtggtaccgc gccgccggcc cggcttacca aagcctcaag ttgcgcgtcg aagccggcgc
    22261 caccctggaa tggctgccgc aggaaaccat cgtctattcc gccgcccagg ctgaactaac
    22321 caccgatatc gaactgcttg gcgatgccaa gctgctctac tgggatatcg tcgcccttgg
    22381 ccgcccagcc agtggcgaac gcttcgacgc cggtcacttc aaagcgcagc tgaatatccg
    22441 ccgcgatggc caactgctct ggcacgagcg ccagcgcgtg gttggcggtg atggtctgct
    22501 cgactcgccc atcggcctgg acggtaaacc ggtcttcgcc accctgctga tcagcggcga
    22561 aatcgacagc gaactgcttg aacactgccg cagcctgaac acacctgtgc gcggcgacct
    22621 gacccaatta cccggcctgc tggtggcccg ctgcctggcc gacgaggcct tgcacgcccg
    22681 cgcctggctg atcgaactct ggcacctgct gcggccggcc ctgctcggcc gcactgccgt
    22741 gcccccacgt atctggagca cctgatggac ctgaccccta gagaaaaaga caaactgctg
    22801 atcttcaccg ccggcttagt agccgaacgc cgactggccc gtggggtaaa actcaactac
    22861 ccggaagcca tggcctatat ctccgccgcg ctgctcgaag gcgcccgtga cggccagacc
    22921 gtggccgagc tgatgcactt cggcaccacc ctgctgagcc gtgatcaggt gatggaaggc
    22981 atcgcggaaa tgatccccga aatccaggtc gaggccactt tcccggacgg caccaagcta
    23041 gtaaccgtcc atcagccgat tgcctgagga aatcacccaa tgcctactgc gcttgcccat
    23101 gcggcgttgc aagccgtctc ggattgctca ttgacagacg tcaactccgc atcctcgccg
    23161 acttgcgcct tgcctgagcg gcgctcgcta cggccttgga cgacttcctg gagacatgaa
    23221 gaatgattat tcgcgatgcc cgcgacgacg acctggaagc catccgcgcc atcttcaatg
    23281 acgccgtgct caacaccacg gcgatctgga tggacgacat cgtcgacctg gccaaccgcc
    23341 aggcctggtt ggccgcgcgc cgccagcaag gctacccggt gctggtggcg gtagacgccg
    23401 acgatagcgt gctcggctac gccgcctacg gcgattggcg gcccttcgac ggcttctgta
    23461 acaccgtgga aaactccctg tacgtacgcg ccgaccagcg cggccgtggc ctcggcgtgc
    23521 tgctgctcgg cgcactgatc aagcgcggca aggccggcgg caagcacgtg atggtcgccg
    23581 ccatcgagag cggcaacctc gcctccatcg gcctgcacca gcgcttcggt ttcagcatca
    23641 ccgggcaaat gccccaggtc ggccgtaagt tcggccgctg gctcgatctg acctttatgc
    23701 aactaaccct tagccccgag aggagcgcgc catgattccc ggcgaatacc agattcagga
    23761 cggcgagatc gaactcaatg ccggccgccg taccctgcgc gtaaccgtgg ccaatagcgg
    23821 cgaccggccg atccaggtcg gctcgcacta tcactttttc gagaccaatg acgccttgag
    23881 cttcgaccgt gctgccgcgc gaggtatgcg cctgaatatc ccggccggca ctgcggtgcg
    23941 cttcgagccg gggcagagcc gtgaagtgga actggtcgac ctggccggcc atcgccgcgt
    24001 attcggcttt gccgggcggg taatgggcga gctgtagggt gcgccctgcg caccacaaac
    24061 aacgcggact ggcggactgg cggactgatg gttcccacgt cgaggcgtcg caccgtccgc
    24121 gtgggaaccc atagcgggac gctccgcgtc ccagcgacgc ggagcgtcgc caactgcatt
    24181 cccacgcgga gcgtgggaac gatcaactca tttgaataac aaggcgaacc catgaaaatt
    24241 tcccgccaag cctatgccga tatgttcggc cccaccgtcg gcgacaaggt gcgcctggcc
    24301 gataccgagc tgtggatcga agtggaaaaa gacttcacca cctacggtga agaagtgaag
    24361 ttcggcggcg gcaaggtgat ccgcgacggc atgggacagg gtcagctctg cgcggcagac
    24421 gtggtcgata cgctgatcac caatgcgcta atcatcgacc actggggcat cgtcaaggcc
    24481 gacgtcggct tgaaggatgg gcgtatcgcc gccatcggca aggccggcaa tccggatatc
    24541 cagccggacg tcacgattgc gattggcgcc ggcaccgaag tgatagccgg tgaaggcatg
    24601 atcctcaccg ccggtggcat cgacacgcat atccacttta tctgcccgca gcagatcgaa
    24661 gaggcgttga tgagcggcgt gaccaccatg atcggcggcg gcaccgggcc ggccaccggt
    24721 accaacgcca ccacttgcac ctccgggccc tggcatatgg cgcgcatgct gcaggccgcc
    24781 gacgccttcc caatgaatct gggttttacc ggcaaaggca acgccagctt gccggagccg
    24841 ctgatcgagc aggtcaaggc cggtgccatc ggccttaagc tgcatgaaga ctggggtacc
    24901 accccggcgg ccatcgacaa ctgcctgagc gtggccgacc aatttgatgt gcaggtggcg
    24961 atccataccg acaccctgaa cgaatccggc tttgtcgaaa ccaccctggc cgcgttcaag
    25021 ggccgcacca tccacaccta ccacaccgaa ggtgccggtg gcggtcacgc gccggacatt
    25081 atcaaggcct gcggtttccc caatgtgctg ccgagttcga ccaacccgac ccgcccgttt
    25141 acccgcaaca ccatcgacga acacctggac atgctgatgg tctgccacca cctcgacccg
    25201 agcatcgccg aggacgtggc ctttgccgaa agccgtatcc gccgcgagac cattgccgcc
    25261 gaagacatcc tgcatgacct cggtgcgttc tcgatgatca gctccgacag ccaggccatg
    25321 ggccgtgtcg gcgaggtgat cacccgcacc tggcaaaccg ccgacaagat gcacaaacag
    25381 cgcggcccac tggaaggcga cggccctggc aacagcaact tccgggttaa acgctatatc
    25441 gccaaataca ccatcaaccc ggcgatcact cacggcatca gccatgaagt gggctccatc
    25501 gaagtgggta aatgggccga cctggtgctc tggcgcccgg cgtttttcgg cgtcaaaccg
    25561 accctgattc tgaaaggcgg ggcgattgcc gcgagcctga tgggcgacgc caacgcctcg
    25621 atccccaccc cgcagccagt gcattaccga ccgatgttcg ccagctacgg cggcagccgg
    25681 catgccacca gcatcacctt tatcagccag gcggcgtttg atgccggcgt acctgagcag
    25741 ctgggtttga agaagaagat cggcgtggtc aaaggctgcc gcgaggtaca gaagaccgac
    25801 ctgatccaca acgactacct gccgactatc gacgtggatc cgcagaccta tcaggtcaag
    25861 gctgacggcc aattactctg gtgtgagccg gccgacgtgc tgccaatggc gcagcgctat
    25921 tttctgttct gaagcctggc ggctgcactc gtcggcaatc tcgagactgc aatggccgca
    25981 gtctatgccg gggctatagt gaaggcttac cccatttccg agagaccgac attatgcacg
    26041 cgccctctcg ctacctactc ggcgccctgt tgctgctcgg cctgccactg gcccaggcgg
    26101 aaacactgac gcccctgcaa ggccagagtt ctgaacagat tcaaagtgat accgccgcct
    26161 gtcaggcgca gtctggaagt agccaacaac aacctgacag caactccgta ggcggagagc
    26221 ggctacgcgg tgcagccaag ggcgccgccg tcggcgcaac ggccgcacag atccgcggca
    26281 atcaacatga cgagatctat gaccgtgtgg atgacgacgt aaaacaacaa tatcggcaaa
    26341 acagggcgcg tgatgcggcg gccgttggtg tggccgtggg cggctcgcgc cagcgccagg
    26401 accgccgcga gaatcgtcgt aacgccgaaa gcagcgccga ttcacagcag gcctacatca
    26461 attgcatgag cgggcgcggc tactctgtag tgccctgatc gtataagccg gtcatgcgcg
    26521 agccggcaga ccaagctagc aaacagtgcg tgtttaaacg gcaaagcccg tacgctaagc
    26581 aagggccacg tggggctcag tttgccgcga ttttctgttc gtcataggtg ctgagatagg
    26641 cacctgagag ttgtttcttt tgcgcctcac tcaataactt gccggccatt tggaaaaact
    26701 tttgctcttc ctcctgcagg tgatggtgca ccttctccga caaggccttc gcctggacca
    26761 accagctagg gctgctgaga tcggtttctt ccagcccctc aaccatttca tccatctcat
    26821 gatgctcggc aatggcatga cgtgaaaggt cgacgcccag gtcatcctgc atcagcggaa
    26881 tataaaagtg ccgctcctcg gccaccgagt gggctgccaa ctcgtcttta agctcggcaa
    26941 acagggcctc gcgctcagga ctatcaccgc tggtcgccac cattttttcg gccaactcac
    27001 gttggcggtc gtggctgatg cgcaaggctt cgaaaattgt catgggttac ctcttccgtg
    27061 atcatcgata caccccgcta tgaccgccat aaccagcaga aagtgccgca ccgcttgttc
    27121 agcgcagctg gctgtccttg ctgccccggc ggttatagcc attgaatttg gcctgttctt
    27181 tgtcgtgctt ggtctggcag gccacgcaca ggcgcacgcc ggggatggct ttgcgccgcg
    27241 cctcggggat ggcggcgtca cattcttcgc aatggcccag gctttcgcca cgcggcagct
    27301 ggcggcgggc gcgctggatg gcgtcctcaa tgctgctgtc gatctgctcc tgtaccgcgc
    27361 cgtcatttgc ccagccaccg gccatcagcc tcgtcctccg ttgtgtgctc ctgtagatat
    27421 gcgccgtgtg ccggcgaatc tcaagagcac gcggtcgcct attccttgac ctgcataaac
    27481 ttgcgcgccc agacgatgta gtcttcgggc tgggtgtagg tatgggtcag ttcagtggcg
    27541 ctaaggtcac ctgcattgat gccgcgctgc tcacgcagac agtcgtaggt agccttgacc
    27601 gccgcgaagt aggccgcgtg accgttgacc acaatgcgca cgcccaggct ggccaggcgc
    27661 gcgttgtcgc gcagcttggg gttgccgtag gtgaccagca tcagcggaat actcagggcg
    27721 gcggcaatct gttccaggtg agcaaaatcc tcgacaccga ccaggcagat ggcatcagca
    27781 ccggccgcct gataggcctg tgtccggcga atcacctcgc tgacctccag caccccggcg
    27841 tgagtacggg cgatgatcgc cagttccgaa tcaacccgcg cttccagggc cgcgcggatc
    27901 ttgccaacac cttcatcaat gctaatcagg tcggtggact tacggccgaa ttgcgcgggc
    27961 aacagggtgt cttcgatggt cagcgcggca atcccggcgc gctccagctc gaccactgtg
    28021 cgcatcacat tgagcgcatt gccgtagccg tggtcggcat cggcgatcac tggcagacgg
    28081 ctgacccggc caatgcgggt ggcctgctcg acgaattcgc tgagggtaat cagggcgaaa
    28141 tcaggtgcgg ccaggacttg tagtgaagct acagaaccgc ccagaatgcc gacttcaaag
    28201 cctagatcag ccgcaatccg agcggccatg ggatcgaata cagaggccgt gtgataacag
    28261 ctatttgccg caagaagcgc gcgaaactgc gcacggaggt cgtgatgcga ggctctttgc
    28321 ataaaatgca ccatcggtca gggtcggtca ggacaagcaa ttatgtagta aaactacatc
    28381 atcaaccagc aaccttgcgc ttgcctttca gtagccgaca acagcgtgct actgcagttc
    28441 gaccggcagt cgcgggccaa agccggcacc agtaagcagc ccataagcca tccacagcat
    28501 cacgccggca tacagcagac gcggcaacca ggtggccagc tgttcggtca ggctatccag
    28561 cttctgggtt tccaccccgg cgtagttaag caggctggcc ggcagggtgc cgctggcctc
    28621 gcccgtgcgg atcagaccaa gggccagccc ctgcccggga aaatcgacag acgccaatgc
    28681 ctgagccagt ccctgaccac gctgaattgc tacctttacg ctgctgaagc gtctgcgcag
    28741 cggcgcatgg ctgatcaccg cgcaggcctt cggcaaggct tcaagcatgg gcatgccagc
    28801 ctccagcatc agtcccaggc tgtcgaagaa atcccgcaga ttacgccgta gcagcgcggg
    28861 gccgacgatc ggcaagctcg ccaacagacc atcaagaggc gaagcagcgc cagcgggcgc
    28921 gtgctcacgc cgcagataaa gcccacgacc cagaccatac aaagccccca gcaccagcaa
    28981 cggcgccagt acgccccaca gataaccggc gatgctcaac tgaccaccga ccaacgccgg
    29041 cagtggctgg ataaacagcg ccagcaccaa cacggccgcc ggcatcagca ggcgtgattt
    29101 tacggcggca acctgttgcg cccgatggtt gtaacggtcg gccagacgct gataaaccgc
    29161 ctccaagcag ccactgacct gagcggcgtg tagcaaggtg ctgtccagct gggtgaacaa
    29221 gccgctgcgc agcccggcac tggccatatc gccgccgcct gcgatctgtg tttgctggtt
    29281 ggctagcgcc gccctggcac gcaccggcac ttgtgcactg agcagcgccc gctcgaccgg
    29341 cacgccggct ttctccatgg ccgccagatg gccaaacaac tgcgcgcgcg cagcaagatc
    29401 aagcagggca ggacgtgagg tgggcatagg caggctcagc gaggagagaa cgacgcaaca
    29461 gacgccagac taacaggccg cgtggcgcgc agcagcggag actattcggt ggtcgtcggt
    29521 aactcggcat ggggcagcca gcgacggaat atctgcgcga aactgccgtc ctggtgcatt
    29581 tcatcgaggg cgcgccgcca acgctcaacc agcgccgcat cggtctgcag ggagaaggcg
    29641 atgtaatagg ccgaacgcat aaagggtaga tgcgcctgca cctgctctgg gcgcaaaccg
    29701 ccggtagcca gttcgtcctt gagggtcagg tcttcagtag cgatcaattg cacccgctca
    29761 tgcttgagca tggtcaccat attcttcgag ctggtgaccc catagatatt cttgaagcca
    29821 cgcgcattca gggtttcaaa ggtgtaccac tgtttcggca ccgccagcgg gccgctctcg
    29881 gcagcctgct gcaggctgtc aaaatgcaag ttgcgcgatt tcagcgagta gaagcttgtg
    29941 gtgccgatca gcagtgggcc gacccactga aatttggcct cacgctccgg ggtgcgcacg
    30001 gtggagaaga tcgccgtatc aggctcgagc tgagccaggt gataggcgcg cgtccagggc
    30061 agcaactcaa tctcaccgga gtcgccggta cgttcgatca acgcacgcac cacctctacg
    30121 gccaaaccgt gcagctcacc atcgcggcta aagctggtcg gcggtgcgtc ctcggtgagc
    30181 aaatgcaaat cggcaagcgc aggcccggcc aaacaggcca gcagcaaaag caacagcgcg
    30241 cagcatggtt tcagcaaagt gggttctccc ggcactggct tcagcctagc tcagtgcagg
    30301 gcaagccgcg ttaccgcgcc aaaggctcgc cagacgccgg catggcgaag ggttgcgcaa
    30361 accagcgctg atgaatgcgc tccaggctac cgtcgcgcag cagctcttcg agggcctgct
    30421 gccattgctg gaccagggcc ggatcggtgc ttttggagaa ggcgatataa gagttgttgc
    30481 ccatcagagt gaactgcaac tcgacctcat cacggcgcat gccttgctgc gcgagcatgt
    30541 catccagcgc caggttgttg gccaccagca actcgatacg gccgtgcttg aacatcttca
    30601 tcatgtgctg gggcgtcggc acgccgtaaa gatttttcag gccacggtcg ctgaggtact
    30661 cataggagta ccactgcttg ggcacagcca gggtgttgaa gcgctcaaca tctttgagcg
    30721 tacgcacatt cagcccggcc ccacgccgcg cataaaagct ggtgtgcccc aaggcaattg
    30781 gccctaccca ctggaacagc gcctcgcgcc tcgcggtgcg caccgtagaa aacaaaccac
    30841 tgttggcctc acgctgcacc tgatggtagg cacgggtcca gggcaccagc tcaatgcggg
    30901 tgtcctgccc ggtccgctga atcagcaact cgaccacttc cacagccatg ccggtcagtt
    30961 tgccatcttc gatatagctc agcggccggt actcctcggt atacaaacgc agctcggccg
    31021 cttgcaccga cgcactgcag ccaagcaaca ccacaccaag ccatgacatc aaccgcattg
    31081 ggtatacctg caaacattaa aacaaccggc tgaagcgcag gatgataaac ggccaccgac
    31141 acaccagcca gcgccgttat gcggcaaatc agcgcgaggg cgacggattg ccggacagct
    31201 cgacctcttg cgcgcgctgc cattgcgcca ggttgtcgcg gctgacctgc cgcgcctgca
    31261 gcgggacgtt taccggttgc agcggcagct caccacgcag acttttcgcc gccaattgga
    31321 cggcctggta accgatggca tgggcttgct gagtcatcag actgtggatt tcacccttac
    31381 tcagagcctc aatcagcaga gggtccgcat caaaaccgat atgcagtaaa tgccccgctt
    31441 tgccgagccg gcgcagcgct gcaagggccg cgcgcgagct agtgctgttg ggcgtaaata
    31501 cgccagccag ctgcgacagt cgatcacgca gcacatccac ggcagcctga ctgtcgtcgc
    31561 cgatataggc atccaccaga atactcagcc caccctgctc cgcgccctgg cgaaagccgc
    31621 gctcacgttc gctggtcgaa ggcacgccag ccttcaagcg caacaaggca acctcgcctt
    31681 gcccgccaag cgccctggcc agagcttgcc ctgcttgcaa accagcgccg aagttatcgg
    31741 tggcgaccac cccgcgcacc gcgctgccgg gcagatcccg gtcgaaatag acagtattaa
    31801 taccgtgatc tgccaattgc tgcacacgcg gcacaatctc agacccactg ggggcgataa
    31861 ccagtgcctt gcagccatgc tccacgaccc agttgatcat ctgcagctgt gtttccaccc
    31921 gcccttcatg aaggggaccg cgaaaatgca catccagcgc atactgctgt cctgcctgcc
    31981 gcgcaccggc ctccacttgc ccccagaagg gcgtggcgcc agccgccact acaccaatac
    32041 agtcctttgc taatgcttga gcgacccaac tggtcaacac cacgaacagc agcacatatt
    32101 tagatgggca tcggcggtac atcagcatca ctcccaaaac ctggcgacgc cccacagaga
    32161 gctgaacagc gcgcgagcat caccttagac tgactaatat cactttagca tcaggcgatt
    32221 aggttggctg agcgtaggac tagcggtcat ccgccagaaa actacgccat acacctgcgc
    32281 cttacacttt ttcacactcc aggcgacggc tgggctggta gccgccgttc aagcaggcga
    32341 tgatcacgtc tctttcctgc accttgaatg acatgaccga taccacgcaa agtactcagg
    32401 ccacgcacaa accccgtccg ctgatcgatt tactggtcag catcctgatt ccctccctga
    32461 tcctgatgaa actcagtggc gagagccgac tgggcgctga tggcgcgctg atgctggcgc
    32521 tggccttccc gctgggttgg ggcagtttcg agctgctcaa gtaccgcaaa ttcaacttta
    32581 tcgccctgct cggcctgatc agcgtgctgc ttaccggcgg catcggcctg ctgaaactgg
    32641 acaaccagtg gctggcgatc aaggaagcgg caatccccgg catcatcggc atagccgtgc
    32701 tggtatcgac ccgcacccgc tacccgctga tccgcaccat gctgttcaac aaaaccgtgc
    32761 tcaacgtcga caagatccat gagcgcctgg agcaggccgg caataccgag ctgttcgaaa
    32821 cgcgcctgct ccgcgccacc tactggctga gcggcacctt cttcttctct tcggcgatga
    32881 actacgtgct ggccaagtgg atcgtcatca gcccggctgg cagcgaagcc tttaacgccg
    32941 agctggggcg gatgaatctg ctcagctacc cgatgatcgc gatcccgtcg atgatcatgc
    33001 tgatggccat cttttattac ctgtggcgca ctattcacgg cctcaccggc cttaagctgg
    33061 aagacgtgat ggccaatgcc gagcaggaaa agcagcccta agcgacgcgc cacgaatgct
    33121 gacgctctcc gcctacctgg gcctgtttat ttcggccctc ggcgctgcca ccctgctgcc
    33181 gctgcagtcg gaaagcgtgc tggtggcgct gctgctcagc ggtgcctatt cgccctgggc
    33241 cctagtggcg gtggccagcg tcggcaatat tctcggttcg ctgctcaact ggttgctcgg
    33301 ccgctacctc gaacactttc gccacaagcc ctggttcccg gtcaccgatg cacgcctgca
    33361 gcaggcgcaa cgtggttatg cacgttatgg ccgctggtca ctgctactga gctgggtgcc
    33421 tatcatcggc gacccgctga cgctggtggc cggggtgatg cgcgaacgcc tgtgggtgtt
    33481 cctgctgatc gtcagcctgg cgaaaacggc ccgctatgct gtattggcag ccctgaccct
    33541 tggctggagt tgagtcgcac gcgacacacg ccgtaacgcc aacagcaagt ccgaggttga
    33601 gcatcggcca tgcctccgcg atactgcgca gccttcgcaa cggccgtgtg cggggccaat
    33661 cgcagagcga aggagtgatc tcagtggagc tgcatccgtg atgaaaagga gcgatctgat
    33721 ctggaccctg gtgggtctcg gagctgttct gctgtcgtgc tacttgctct atcaccaagt
    33781 gcgcactatc tcgctggatg aaatcgctga cagcctgcgc gccattccgc gcctggactg
    33841 gctactggct gcagcagcca ccctcggcgc ctatagcgcc ctagcctggt acgaccgtat
    33901 cgccctggcc cacctgggca aaaaaatccc ctggcgcttt atcagcctgt gctccttcac
    33961 cacctatgcc ctggcccaca atattggtgc ctctgtgttc tccggcgcac tggtgcgtta
    34021 ccgcgcctac cgcagcaaag gcatgacgcc gcaagaaatc ggcatcctga ttgtgttttg
    34081 ctccttcacc ttcgtcctcg gcaccatcct ggccagcggc tgcgtactgg tgctgcagcc
    34141 ggacctgatc caccgcgtgg ccaaggttac gccgctggta tcagagatca ttggcgggct
    34201 cctgctgctg ttggtcgccc tctacgtgct cggatcctgg cgccacttca aaccctggca
    34261 ctggggcaaa tttcatctcg aatacccgcg cctgggtatt gttggccgcc agttactggc
    34321 gggcccgctg gagctggcct gcgcggcggc gatcatctac ttcgccctgc cggcagagaa
    34381 caaccctggc tatctggtag tgttcggcgt gttcctcgcc tcattctcac tggcgttgct
    34441 gtctcacgcc cctggcggcc tgggcgtact ggaagtgacc ttccttgcag cgatgcccga
    34501 gctgccagcg gctgacgtgc tcgccgcgtt gatcgtgttc cgctgcttct acctgttgtt
    34561 gccattcgcc ctgtcgctgc tggtggtgct gggctttgag tggacgcagt ggcagagccg
    34621 ccgtacaccg ccgaacaccc cgccccaacc ctgagccgaa taaacaccgg ataaccgggg
    34681 gcagcattgc gccgcgcact cgcatagaat gcgcggccgt ttttgcggcc aagcctttat
    34741 ggcggccagc caccaggccg tcgccacagg acgcccagaa acctcccggt acttattcag
    34801 gaacagcgcg ccgcatgact ttctctacac ccgcccgcgc ctgcggcatc gacttcggca
    34861 cctccaactc caccgtcggc tggctacgcc ccgatgcaga gaccctgatt cccctggaag
    34921 acggcaagat caccctgccc tcggtggtgt tcttcaacct cgaagaacgc cgcccggtct
    34981 acggccgcct ggccctgcag gaatacctgg aaggctacga aggccgcctg atgcgctcgc
    35041 tgaaaagcct gctgggctcc aagctgctga aaagcgaaac caccgtgctc ggcagcgccc
    35101 tgccgttcaa ggacctgctc ggcttcttta tcggcgagct gaaaaatcgc gcagaggcca
    35161 ccgcgggccg cccctttgaa gaagtggtgc tgggccgccc ggtatttttc gtcgatgacg
    35221 acccggtggc cgatctggaa gcgcagaaca ccctggtagc ggtggcgcac aagctcggct
    35281 tcaaggatgt gtcgttccag tacgaaccaa ttgctgcagc cttcgactat gagtccggca
    35341 tagaacgtga agagctggtg ctgatcgtcg atatcggcgg tggtacctcg gacttttccc
    35401 ttgtgcgcct ggcacccgaa cgccgcacag tggcggaccg gcatgccgat atcctcgcca
    35461 ccggcggcgt gcatattggc ggtactgatt tcgacaagca gctctccctg gccggggtaa
    35521 tgccgctgtt cggctacggc agccggatga agagcgatgc gccgatgccc accagcaccc
    35581 acctgaacct ggccacctgg cacaccatca atgcggtgta ctcggcgcag tcccagcgcc
    35641 agttgcagag catgcgctac gacatcgtcg acccgacggg catcgaccgc ctattccagc
    35701 tgatcgaacg ccgcgacggc cactggctgg ccatgcaggt ggaagaaagc aagatcgcac
    35761 tgaccgaaca ggatgcacgg cccatcgacc tcagccgcct ggaagacggc ctagtcgccg
    35821 agctgacccg cgagctgttt gaaggcgcca tcgaaccgct gttggaacgc gtgcgtgcca
    35881 gcgtcacgca gctgttaagc gatgccagcg tcagtgttgc ccaggtcgat acggtgttct
    35941 ttactggcgg ctccagcggc atcccggctt tgcgccagag cgttgcagcg atgctgccca
    36001 acgcccgcca tgtggaaggc aacaccttcg gcagcatcgg cagcggcctg gccatcgaag
    36061 cgaagaagcg ttacggctga ttagcgcccg gcaccataaa aaacgccagc tgaacgctgg
    36121 cgttttttat tcctgcttaa tcagcgcggc acggccggct tcttcaccgg cttcttgcct
    36181 ttgcccttgg ccgcgtcggc acgttccttg gccgcctgct ggttacgcac ctgcgcggcc
    36241 gccttggcct gctcacgctt gtcccatggg ttaccacctg cagcaccggt atcacgcggc
    36301 ggcaagccgg tgtgctgggt cagcatcggc ttgctcttgg tggtgtcctt ggcgaccttg
    36361 tggctaccgg ccggcgtcga atttttacgg cgcgcgctct ggtagctgtc ggtagcaggt
    36421 tgatgcagcg ggataagctg attcttgccc ggcccgatca aatcggcacg gcccatgcga
    36481 gtcagcgctt cgcgcagcat cggccagcct ttcgggtcgt gatagcgcag gaaggccttg
    36541 tgcaggcgac gctgctcttc gctcttgacg atggtcacgc cgtcgctctt gtaactgacc
    36601 ttgcgcagcg ggttcttgcc cgagtggtac atggccgtgg cagtggccat cggcgacgga
    36661 tagaaggcct gcacctggtc ggcgcggaag ccattaccct tgagccacag ggccaggttc
    36721 atcatgtctt cgtcggtggt acccgggtgt gcggcgataa agtacgggat caggtactgc
    36781 tctttgcccg cctctttcga gtacttctcg aacatgcgct tgaacttgtc atagctgcca
    36841 atgcccggct tcatcatctg attgagcgga ccttcctcgg tgtgttccgg ggcgatcttc
    36901 aggtaaccac cgacgtggtg ggtgaccagc tctttaacgt attccggcga ctcgaccgcg
    36961 aggtcgtagc gcagacccga agcgatcaaa atcttcttca cccccggcaa tgcgcgggca
    37021 ctgcggtaca gctgaatcag gctggagtga tcggtattca gattcgggca gataccgggg
    37081 aacacacagg acggcttgcg gcacgcggac tcgatctccg ggctcttgca ggcgatgcgg
    37141 tacatgttgg cggtcggccc gccgaggtcg gaaatcaccc cggtaaagcc cggcaccttg
    37201 tcgcggattt cttcaatctc gcgaatgatc gactcttccg agcggttctg gatgatgcgg
    37261 ccttcgtgct cggtaataga gcagaaggta cagccgccga agcagccacg catgatgttt
    37321 accgagaaac ggatcatgtc gtaggccgga atcttttcct tgccgtatgc cgggtgcggg
    37381 acgcgcgcat agggcatgcc gaacacgtaa tccatctctt cggtggtcat gggaatgggt
    37441 ggtgggttaa accacacatc cacctcacca tgcttctgca ccagcgcgcg ggcgttgccg
    37501 gggttggttt ccaggtgcag cacgcggttg gcgtgggcgt agagcaccgg gtcgttgcgc
    37561 accttttcca tcgacggcag gcgaatcacc gttttgtcgc gggtcatccg cgggctggcg
    37621 agaatctgca cgaccttggg ttcgttcgga tcctcctgcg ggcctttttc ctgctcgatg
    37681 gcgcaagcct gggtgtcctg ggtgttgacg tacgggttga tgatcttgtc gaccttgccc
    37741 ggacggtcga tgcgggtgga gtccacctcg taccagccct gcggcgtgtc gcggcggata
    37801 aacgcagtgc cgcgcacatc ggtgatgtct tcaatcttgt ggccgtagga caggcgctgg
    37861 gccacttcga cgatggcgcg ctcggcgttg ccgtacagca ggatatcggc gctggcatcg
    37921 atcaggatcg agttgcgcac gcggtcctgc cagtagtcgt agtgggcgat gcggcgcagc
    37981 gaggcttcga taccgccgag cacgatcggc acgtttttat aggcttcttt gcaacgctgg
    38041 ctgtacacca ggctggcgcg atccgggcgc ttgccggcca tgccgccggg ggtgtaggcg
    38101 tcgtcggaac ggattttctt gtcggcggtg tagcggttga tcatcgagtc catgttgccg
    38161 gccgcgacgc cgaagaacag gttcggctcg ccgagcttca taaagtcgtc tttcgactgc
    38221 cagttcggct gggcgatgat gcccacgcga aagccttgcg actccagcag ccggccgata
    38281 atcgccatgc caaacgatgg gtgatccaca taggcatcac cggtgacgat gatgatgtcg
    38341 cagctgtccc agccaagctg atccatctct tcccggctca tcggcaggaa aggcgcaggc
    38401 ccgaaacact cggcccagta ctttggataa tcgaataacg gcttggcggt ttgcatggac
    38461 atgaaaagtg accggcgttg gcgaacaggg atggaaaatc gcgggcgcgg aatatagcac
    38521 aaattttaac caaacccgac tcctccgctc gggcatttac ctcccttcat cccccacata
    38581 cccgcctcca cccctatcca aaagcgggtg cgcgcggcca ggcatcacag ttactgggtc
    38641 gtttggctgc acatcaccca gggcgggaaa cagcccaggg cataaattcg cagccgccga
    38701 actttggatc aatggccccg cccgacggtg aactgaacac tctcggcgcg cacatttcaa
    38761 caagaccagt gcccccgacg actcgtgctt ggcgacccgt cgcggtatgc aggcccaccc
    38821 agctgtgtga ggcggcacct gccctaaaca ataaaaacaa cacgttcact gagggcatct
    38881 ctcatgctta cacctgccga aaaactgagc agccgctacg tacccttgca gcgcctgagc
    38941 cctgccgata cacagcagat gtacgccgtc ttctcgcagt actaccagaa caccacgcag
    39001 cagaccttcc tcaacgacct gtcgcgcaag agcggcgtaa tcctcgcgcg cacccgcaag
    39061 agcaagcgta tcgtcggttt ctcgaccctc accgacttcg aaatcactgt cgacggcaaa
    39121 cccgccaggg gcctgttcag cggtgacacc atagtcgagc cggcctactg gggcgctcgc
    39181 tgcctgcacg tggcgttcta cctgcaactg atgcgcgtac gtatgcgcta tccactgacg
    39241 ccgctctact ggttgttgat ttccaagggc tacaaaacct atctgctgct gacccgtaac
    39301 ttctacgcgt acttccctga ccgtaatacg cccaatagcc ctctccagca ggtcgtggcg
    39361 gcctacagcc agcagctctt ccccgatgcc ttcgacccgc aacgcatgct gctggacttc
    39421 ggcgaaggtg cgcagcacct gaaggacgaa gtcgccggca tcacccctga gatgtgcgcg
    39481 caggacgccg acatcgccta tttcgagcag tgcaatccgt cctggcggcg gggcaccgaa
    39541 ctgccctgcg ccggtgaagt gtcctattcc gccctgttgc gctactgctc ccgcctgggc
    39601 ctgaagctgt tgcgccgcaa cctgaattgg cgagcggccg cgactgccgc gcagaaccag
    39661 gcacaggcca acaccgcaag ggacggcgcg accgagctga gccaatgacc gcagtgattg
    39721 ccagccgttg cggccatggc ctgctcagag catttatgca cagcgggatg cagcgtttcg
    39781 aacgtcaggc ccacgacctc gaagccgtgc agcgccgcct gctgcgcgac tggagccaac
    39841 gcttcgtacg ctccccgcgc ggccgcaaca gcggcatccg ccccgaagac agctgggagc
    39901 aggtgaccgc atgcctgccc agcagccact acagcgacta tcaggacgat ctcgaacggc
    39961 aacgccggga aaacaaccaa cgcctgatcg acagcccgat cagccgctac cagccaacca
    40021 gcggctcgac ctcggcgatc aagtggattc cctacacccg aacctttctc gccgaactcg
    40081 accgggcgat cagcccctgg atcggcgacc tctatcggca gtacccgcag atcgcccaag
    40141 gcagccacta ctggtcgctg tcatggattc caagcgcact acgcgatacc agtagcgccg
    40201 acctcaacga cgacatgcaa ttgatgtcgc tgggtaaacg cctgatcgcc ggcctgaccc
    40261 aaaccgcgcc accaggcatt gccctggccg gcagcgccga cgactcgcta ttcgccacgc
    40321 tgtccttcct cgctgcggat gcacgcctga gcgtgatgtc gatctggagc ccgaccttcg
    40381 gcctcggcct gctggaacaa ctgggccatt gccgcgaaga gctggccgag gtgctcagca
    40441 gtggccattg gggccgacgt agcgccgcca tggccgcgct gcgctgtccg cgctcaacgg
    40501 ccgccgccca gcgtctgcgc gcctgggatg gcaacgcgga tccggcattc tttgccgaac
    40561 tctggccgca gttggcgctg gtcagcgcct gggacaccgc cgccgccgcg ccctgggccc
    40621 agcgcctgca acagctactg ccgcaggccc gctttcaagg caagggcctg tgggccaccg
    40681 aaggcgtggt caccttgccg tttcgcggcc gctacccact ggcctaccgc agtcatgtct
    40741 atgaattcga agacctcgac aacggccgga ttctgccgcc ctgggccctg cgccagggcc
    40801 agcaggtgat gccgctgctg accaccggca gcggcttcgc ccgctaccgg atgaatgacg
    40861 ttttggaggt cagcggccac tggcgcacgc tgccctgctt ccgctttctc gggcgcaacg
    40921 atggcgtcga tctggtcggc gaaaaaatca gcgcaaccct ggcgcaaact gtgctcgacc
    40981 gcctcgacta tgccggccgc ctgccgatca ccctgctggc cctggacgac agcagcggcg
    41041 gccgccccgg ctatgtgctg ctgctggaaa gcagcgccga gcagagcttg cccgccctgc
    41101 agagcctggc ccagcagctg gaaaacgctt tgctcgacaa cttccactac cgcctggccc
    41161 gtgacctcgg ccagctcgcc ccagcgcgct gcatcgccca gccgcagatg cgccagctgt
    41221 acctggagca ggcacgggcg cgcggcatgc tggagggcaa tatcaagctc gaagccctgc
    41281 gccactggcc cggtgaactg cccgccagcc tgcgccacct gctgcaggtg agctgatgcc
    41341 ggacactcac gagattctgg tgcggccggc ccggcccgaa gacaattcgg cgctgctcga
    41401 cctgctacgg cgtacgccgc agatgggcag cgttaccctg accttcgaac gcgagccgga
    41461 ttttttcagc ggcgcgcggg tggcctgcga agtgccgcgg gtactggtcg ccgagcggcg
    41521 cgagcaccca ggccggttgc tcggcatggt ggatatcggc caacgccagc agtacctcga
    41581 cggccagcca tgcacggtca gctacctgca tgacctgcgc atcgccgccg aagggcgcgg
    41641 cggccgtgtg ttggccgcac tgtttcgcgc catcgccacg gccatgcaag cacccggcag
    41701 ctggatggag gctgtgatcc tcgaagacaa cctggtgccc ctgggcatcg tcggccgcgg
    41761 ccgcgctggg ctgcccaact tctatccgaa cgggcgcatc accaccagtc tgttgcccgg
    41821 ccgccagtcc cgcagccaag ccgacagcgg tctgcagatc cgcgccgcga gcgctgcgga
    41881 tgcacagcag ctgcaacagc tgtggcaaca ggctggcagc cgtcaaggct ttcccgtcta
    41941 tcaggtccag gacctgctgg aaggcagccc gtactatcag gggctgcaga ttgaggacta
    42001 ctggctagcc tgtatcgacc agcgcgtggt cggggtggtc ggggtgtgga accagaaggc
    42061 cttcaagcag acccgcgtgg cccgctacag cccctggcta gccgccctgc gcaggcccta
    42121 caacctctac agccagttgt tcggcgggtt gcacctgccc gccgtcggca acaccttcaa
    42181 ctatctgaac ctgcacgggt tgagcgtgct gaacaacgac ccgctgattt tccaggcgct
    42241 gctcgagcat gttctggccc accaggtaca ggcccaacaa agcctggtgt gcggcttctt
    42301 cgacgacgac cctctggcgc aagtcgtacg cccttaccgg cgtcagaccc tgcacagctg
    42361 ccacttcctg ctcagctacg accacgatcc gcgcccggca caggcgcgtg gcccgcgcca
    42421 tgtcgaaatc gcccgcctgt gagaatcgcc cgaccatgac ccacgccgaa cctgcacgcc
    42481 ccctcgccac cacaccggtc gacctggacc cgggctacgc catggatgcc aagaccctgc
    42541 gccgcctgtc cctgttcgcc gccgtgctgc tctgcatcgc cctggctttc ggggcgtact
    42601 ggctgctcca cgcgcgcttt ctcgaaagca ccgacaacgc ctatgtgcgc gccgatatcg
    42661 tggtggtttc cagccagctt gccgcacgca tcacccgcgt acaggtggag gacaaccaat
    42721 gggtcgaggc cggcgacacc ctggcccgcc tcgacgacgc cgacttcgtt tatcaggagc
    42781 aacgcatcca gacccagatc gacgacctgc aggttcagct gcacagcctg accaatcagc
    42841 acgcgcagca gcaggcgcgg atcgaggcgc aggccgccga ggtccaggcc agcagtgccg
    42901 aactcggccg ccagcagggc aacctcgaac gcattcgcgc cctgcgccgg ggcggcttcg
    42961 cctcggagca acagttctcc agcctcggcg ccaatgtcgc ggtggcccgc gccagcgtcg
    43021 acaaggccaa cgccggactg aagggccaac gcctggagct cagccagctg gataaccgcc
    43081 gccagcaact gcagaaccag cttgacgggg cccgcaacga cctggcccag gcacgcctga
    43141 acctagcccg tacgcaaatc gtcgcgccga tagccggacg cgtcggcaac cgctcgctgc
    43201 gtgaagggca gtacgtgcgt ccaggcgagg cactgctcgc cctggtaccg cgcagcgtct
    43261 gggtgcaggc caacttcaag gaaacccagg tcgcacgtat gcatccgcag caaaaggtcg
    43321 agctgcgctt cgacgccctc cccgagctgc gcctgcaagg caccctcgac agtctgtatc
    43381 ccgccaccgg tgccgagttc agcctgctgc cgccggacaa cgccaccggt aacttcacca
    43441 aggtgatcca gcgcgtgccg gtgaagatcc gcctgccgga gaacattccc gaactggagc
    43501 gcattcgtcc cggcatgtca gtagtggcga cggtggacag ccgtgactga gcaaccgcta
    43561 gcgcaacgtg aacccagtct gcgcgactgg atagcggtcc tcagtgcaat gcttggggcc
    43621 tttatggcgg tcatcgacat ccttatcacc aacgcctcgc tcaaggagat ccaggccgcc
    43681 ctcggcgcca ccctcagcga aggttcgtgg atctccacct cttatctggt cgcggagatc
    43741 atcatcatcc cgctgaccgt ctggctggtc gacctgcttg gcgcgcgccg tctgactgtc
    43801 tggctgacaa tcggttttat cggctcctcg ctgctctgct cgatggcctg gtcgctgcac
    43861 agcatgatca cctttcgcgc cctgcaaggg ctgtgcggtg gggcctttat tccgctggcc
    43921 ttcagcatgg tgctgacgcg tctgccgccc catcgtcgcc cgatcggcat ggcgctgttc
    43981 gctgtcaccg ccaccttcgc cccggccatc ggcccgtcca tcggcggttg gctgacggag
    44041 cacctaggct ggcaatacct gttctacatc aacctgccgc cgggcctgct gatgatctgc
    44101 gggctgctct acggcctgga aaaacgcgcc atcgactggc ggcagatacg ccgcgcggac
    44161 tacgcaggga tctgcagcat ggccatcggc ctgggctgcc tgcaggtgtt tctcgaggag
    44221 ggttaccgca gtgactggtt cagctcgcgg atgatcgtgg tgctggcact gatcgccttc
    44281 gtcagccttg gctgctttgt catcatccag ctgtcccgcg agcatccgct gctcaacctg
    44341 cgcattctca agaaccgcaa cttcgccctc agttgtgtcg ccgccgtggg cctgggcatc
    44401 ggcctgtatg gttcgatcta cgcgctgccg ctctacctgt cgcagatcca gcactacagc
    44461 ccgctgcaga tcggcgagct gatcatgtgg atgggcctgc cgcaattggc actgatcccc
    44521 ttcgtaccgc tgctgatgcg ggttatcgac ccacgcctgt tgtgcgccat tggttttatg
    44581 ctgttcggct atgccagctt tatgagcggc tcgctcaacc ccgacctatc aggcccacag
    44641 ctactgcata tccagctgat ccgcgctatt ggccagccgc tgattctgat cacttcgtcg
    44701 ctgatcgcca ccagctacat cgaacacaag gacgccggct ccgcctcggc gctgttcaat
    44761 atcctgcgca atctcagtgg cgcgatcttt atcgccctgc tgatgaccat gctgcagaac
    44821 gacaccagcc gcaattacca atacctcagt gagtcagtca gcaacctcaa cccccaagcc
    44881 ctggcccggc tcgaatggct cacggagaag ctcggcagca gccaacgcgc cctcgccgtg
    44941 ctcagccagg aaacccgcga acaggcctcg atccttgcct acaacgaggc cttccatatc
    45001 atgggctgcg cgctggtgat ctgcgtgctg gcgatgctgt tcactcgccg cctgccgcca
    45061 ggcctgaaac ctggagaggt gcactgaagc aacgtgaaca tgggacgcat aagcaaccgt
    45121 cagaatgggg caaaccgacc gctaagcttg tgatcacagt cgtgcttgtg gctatactcg
    45181 acccgataaa agcatcggca tcgatagcca ggtttacgga gtgcatgtag tgcagcgatt
    45241 tgtctcctca atcgtcctgc tgctcggcct ggcggccggt gtgtgcgcag ctcccgccct
    45301 gctgaaagac gacagcagcg gcagctctct gaatgagcat atcgagctgc tggaagacgt
    45361 cggcgggcaa atgagcattg ccgatatggc cgatccggcc gtgcaaagcc gcttcacacc
    45421 ggctaatggc cgcgccagcg ttggccagag ccgcaatcct tggtggatca agctcaccct
    45481 gcagcgcagc gcagatgccc caagccagtg gtggctggaa gttagctcgg taacccagtt
    45541 cgacctacga ctatacttgc cagatcagaa tggcaattgg caggaacgcc aatccggcga
    45601 actggtcagc cacaaggaag gccgcgatca cgaattccgc cacatgctgt tcaagctacc
    45661 ggagctggat caggaaccac tcaccctcta cctgcgcagc tatgaccccg caggtaactc
    45721 gttcccgatg cgcgcctggc aactggctga cctgacccag caagccgccg aaaaaaacct
    45781 cgctctcggt gtggtctacg gcatcatcgc cgccctgctg ctgtacaacc tgtttatctt
    45841 tttcagcctg cgcgactcgg cctacttctg gtacgtactg accaccgccg gtgctctgct
    45901 aatgattctc agcatgagcg gtcatggctt ccagtaccta tggccgaaca accccgtacc
    45961 cttttggttg gatcgtgtca ccctgccatc gttgtggggc ttctgcgcct gtcgctttac
    46021 ccagtgcctg atgcaaaccc gcacgcatgt acgctgggcc catcatctgt tgagcctggc
    46081 ctgcgtctgc tatgtcattg cggtcgccct ggagggcctg gggcaacgcc atacggccgc
    46141 ctggatcatc gccctgctgt cactgaccag catcccggca gcgctgggat cggccctgat
    46201 ccgctggcgc cagggctact tcccggcctt gctctatctt tgcggctacg gcctgattct
    46261 cggcagcatc agcctggccc tgatgcgcgc cactggcctg gtgcagccgg caacctggaa
    46321 tgcctacgtg tttccactgt ctgtggcggc cgagtcgatt ctgttttcct tcgccctggc
    46381 gtatcgcatc cagacgctca agcaggaaaa agccgaagcc ttggtacagg cagaccgcga
    46441 aaaaaccgca cgcctagcac agatgcaggg cagcgctgat gaactgcaaa atgccgtcaa
    46501 actacgtact gcagaattgg cgctggccaa ccaacagctc agcgagcgcg agcgcgagtt
    46561 gcagcacgcc gccttccacg acccgctgac cgagctgccc aaccgccgct acctagtcga
    46621 gcgctgcgaa gccgccctgg ccgaggccaa acgccacaac caggcggtgg ccctgctgct
    46681 gatcgacctc gaccacttca agccgatcaa cgacagacac ggccacgacg ccggcgacca
    46741 gatgctccag gccatcgccc agcgcctgcg cgagcatgtc cgcgcaggcg acgccgtggc
    46801 acgcctgggc ggcgatgaat tcgccgtgct gatctgcggc gcggatgctg aacagcacgc
    46861 tcgggaaatt gcccggcgcc tgctggatga actggccaag ccggtaacct acggcgcggc
    46921 acgcctgacc gtgaccatca gcatcggcgt ggccctctat ccgcgccacg ccagcaactt
    46981 caccacgctg tacaaggccg ccgatgaaat gctctacaag gtcaaaagcc gcggtcgctc
    47041 tggctcggct gtttgcgggg aagacggcga gctgagcagc agcgcccgcc tgcaggtgga
    47101 cgtgctgaat gtgcccagcg gcctgggctg attgctcaaa gacagtggtc gctgacagcg
    47161 ccccggttat cgccaaacca agaactgtga acgattaccc ggctgcgcct atactcgcgc
    47221 caaacgccac tcgacatcgg gaagtccgca gtgcagcgcc taaccttagc cattctcctg
    47281 tttctgagcc tatttggcac cctggcctgt gccagcccgg tgctgctgag cgcgccgagt
    47341 agcggcagca tgctcaatgg ccagatcgag ctgctggaag atgtgggcgg ccagctgagc
    47401 attgccgata tggccgaccc cgccgtgcaa agccgttttc agccggccgc cgggcgcacc
    47461 agcgttggtc agagcagcaa cccctggtgg atcaagctca gcctgcaacg cgccattgac
    47521 gcgcccgcgc agtggtggct ggaagtcggt gcggtcaccc tgctcgacct gcaactcttc
    47581 ctgcccgacg accagggcgg ttggcaaaca cgccaggccg gcgagcgtgt cagctttgcc
    47641 gaaggccgcg accacgatta ccgccgcgcg gtgttcagcc tgccgcccct gggcgagcag
    47701 ccgcagaccg tctatctgcg cacctatgac ccggccggca actccttccc gctgcgcatc
    47761 tggcagctgc atgacctcga acaactggcc aagcgcgaac acctgatcct cggcctgatc
    47821 tacggggtga tcctcgctct gctgctgtac aacctgttta ttctgctggc cctgcgcgac
    47881 ccggcgtacc tctggtatgt gctgaccatg gcggcttcgc tggtgtttat cgccagcatg
    47941 agcggccatg gcttccagta cctctggccc gatggtccgg tgcccacctg gctggatcgc
    48001 atcaccctgc ccattctggt cagcctgggc atcctgcgtt ttgctcagga gctgctatcc
    48061 acccgcacca ccttgcgcat tgcccatcac atcctcaacg gctgtatcgc ggtctacctg
    48121 ctggccctgg cggccaacct ggccggctat cgcagtgaaa cgggcctgct gattgggctg
    48181 atcccgattg ttaccgtgcc cacccttcta ctcagcgcgg tcatccgctg gaaacagggt
    48241 tttacccctg cgttgttcta cctgctgggc tacggaagcg tactgctgag ctttgtgatt
    48301 ctggtgctgc gcgccgccgg cgtggtgcaa ccgggcgaat ccactgccta tctgttccca
    48361 ctggcggtgg cggccgaagc gatcctgttc tccttcgccc tggcctatcg catccagatt
    48421 cttaagcagg aaaaggctga cgcgatcctc caggccgacc gcgaaaaaac cgcgcgcctg
    48481 gcgctggctc aggccaatgc cgccgacctg cagcgctcgg ttgaattgcg taccgctgaa
    48541 ctggccgcca ccaatgaacg cctgcgtcag cgtgaacagg cgctgcagca cgcggcgttt
    48601 cacgactcgc tgaccgagct gcctaaccgt cgctacctgg tcgagcgctg cgaagccgcg
    48661 ctggccaatg cccgccggca tagcgaaacg attgccctac tactgatcga cctcgaccac
    48721 ttcaagccga tcaacgacaa acacggccat gatgccggcg acctgatgct gcagcagatc
    48781 gctctacgcc tgcgcgagca tgtgcgcgca ggcgatgccg tggcgcgcct gggcggcgat
    48841 gagtttgctg tactggtgtg tggcgccgat gccgaagagc atgcgcggga aatcgccaat
    48901 cgcctgctcg ccgaattggc caagccggtg atgtaccgcg ccgaacggtt gaccgtaacc
    48961 atcagcattg gtgtggcgct gtacccacaa catgccggca cttttaccgc gctgtacaaa
    49021 gcggccgacg agatgcttta tcgggtcaag gcacgcggtc gctcgggctc ggcggtatgc
    49081 ggcgaagacg gtaagctgag tgacagtacg cgcctgcatc tggatgtgct gagcgtaccc
    49141 agcggcctgc tctgactcag ctgctgcgat aacgccgtgg cacccgcgcg gtgaccttgc
    49201 tgagcagctc ataaccaatg gtgccggcag cctgcgccac ctcatccacc ggtaattgcg
    49261 aaccccacag ctcgactgca tcgcccactg ccgcagcagg caggtcggtg aggtcgacgg
    49321 tcaacatatc catggacacc cgcccgacca gcgcaacccg ctgcccctgg atgaccacag
    49381 gggttccggc aggcgcatgg cgcgggtagc catcggcata accacaactc accgtcccga
    49441 tacgcgacgg ccgctcggcg cgccagtttc cgccatagcc aactgtttca cccaccgcta
    49501 cagcgcggca ggcaatcagc tgcgctgtca ggctcatcgc cgggcgcagg cctagctcag
    49561 cgacgcccag gtcagcacaa ggcgtcgcac cgtaaagcat gatgcccgga cgcagccagt
    49621 ccatatgcgc ggccgggatg gtcagaatcg ctgccgagtt agccagcgag cgctgggcga
    49681 aatccagatc cagcagatcg agaaactgct caagctgcag ttcggtgact tcgctgccgc
    49741 gctcgtcagc gcaagcgaag tgactgatca gattcagctc ggccacctgc gccacgccct
    49801 gcaaacggcc gtgccattca cgcactgccg cggagctgaa cccaaggcga tgcataccgc
    49861 tgtccagctt caaccagaca ttcagcggcc gcgtcaggcc ggcggcgagc agctgttgcg
    49921 cctgctgctc gctgtgcaga accacatcca ggcccagctg cgcggcaaac tggtactcgt
    49981 ccgcctcaaa gcagccttca agcaacagaa tccgcgcctc accgtgcagc gcacgcacct
    50041 cggcggcttc ctccaggctg gccacggcaa agccgtcagc cacatcgtgc agggccgtca
    50101 ccacttcgcg cacgccatgc ccgtaggcat tcgccttgac caccgcaaac gcctgccgcc
    50161 ccggcgcgca acgcttggcc accgcgtagt tatgggcgat ggctgacaga tcgatggtgg
    50221 caacaagagg acgcatgaca gggctccacg gcaggctaaa gccgagcatc ttaaccctca
    50281 actcccgggc tgcaagcgct aggtagccct gcacaagcta tacacctgcg atgccgctac
    50341 gagcgacgca aatttaatcg cagcggggca aaagctactg acattaagct gtcagtagca
    50401 ctcccctaga gtgggttcca caggctaaaa cgcgccgtca gatactgccg ttgcctgtcg
    50461 caccctaacc ttcaaggaga gagaacatga atgcgatcaa ctggttcgaa ctatttgtca
    50521 gcaacttcga gcgtgcccgt ggtttctatg agcgtgccct ggcaacgccg ttggaagtaa
    50581 tggagggcat gggcgggcgt atggcactgt tcccccatgc acaggaaagc ggtgtcggtg
    50641 gctgcctgag cgagtcagcc gagcaacaga gtggtattgg cggcacccgc gtctacctga
    50701 acgtcgaggg acaactggat gctgtggtga accgggttcc ctcggcaggg ggcgtgatca
    50761 tccatagcaa gatgtccatc gcgccgcatg gctttatcgc tgtgataaaa gacagcgaag
    50821 gtaatgaagt gggcctgcac agcatgtcct aaaaccggca ttccgggcgt gcgtcagcgc
    50881 gcccggtaat ccagagcgag aacagctgag acaacatgag acgcgccgac cgtctatttc
    50941 aaatcgtgca gttcctgcgc agccgtcgcc tgaccaccgc ccagtggctg gccgaacgcc
    51001 tgcaagtatc ggtgcgcact atttatcgcg acatccaaga cctctccctt tctggagtcc
    51061 ccctggaagg tgaagccggc gtcggctacg tgctgcgcca accgatggat ttgccaccct
    51121 tgatgttcga gcgcgaggag atagaggcgt tgctcgtggg cgcacgcatg gtcaaggcct
    51181 gggcggaccc acaactacaa cgggccgccg aatcggcact ggcgaagatc cacagcgtgc
    51241 tgcccaacga gctgcgcgat gagctcaatc gcaaccagct gtttgccccc gaaaccaatc
    51301 cctatcctgt gcattggctc ggccagctgc gccaggctat acgccagcgc cacaaactgc
    51361 tgatcagcta ccgcgatgaa cgtggggaca ccagccaacg cagcgtccgc ccgttagggc
    51421 tgttcttctg ggggcaaacc tggaccctgt gcagctggtg cgagctgcgc aacgactttc
    51481 gcaacttccg catcgaccgc gtggaacagc tgcaagaccg tgcggaaacc tttgaagcag
    51541 tggaaggcca acgccttgag gactacctca aaccctacct gggcgagcac gccaccgcgc
    51601 cgctgtacaa aagctagcgc ctgaggtcgc ggctagaaga caccagatca ctcgtcgtcg
    51661 aactgatagc tacccggcgc gaggttctca aagcgcgaga acttgccaag aaacgccagc
    51721 cgcgccgtac ccagcgggcc gttacgctgc ttgccgataa tgatttcggc cacgcccttg
    51781 tactcggtct ctgggtgata cacctcgtcg cggtaaacga acatgatgat gtcggcgtcc
    51841 tgctcgatcg caccggattc acgcaagtca gagttgatcg ggcgtttgtt cgggcgctgt
    51901 tcgaggccgc ggttgagctg cgacagggcg atgaccgggc agttgaattc cttggccaga
    51961 gccttgagtg agcgggagat ttcggaaatc tcattgactc ggctgtcgcc gctggagcca
    52021 ggaatctgca tcagctgcag gtagtcgacc atgatcaggc caatctcacc atgctcacgc
    52081 gccagacgtc gggtacgtgc gcgcatctcc gagggcgaga tgccggcggt atcgtcgata
    52141 aacagcttgc ggtcgttgag caggttgacc gcgctggtca aacgcggcca atcgtcgtcg
    52201 tccaggcgac cagcacgcac cttggtctgg tcgatacgcc caagcgaggc cagcatacgc
    52261 atcacgatgg agtcggacgg catctccagc gagtacacca tcaccacctt gtcgctgcgc
    52321 atcagcgcgt tttccaccag gttcatggcg aaggtggtct tccccatcga cggtcgaccg
    52381 gcgacgatga tcaggtccgc cggctgcagg ccgctggtca ggtcgtccag gtcggtaaaa
    52441 ccggtggaca ggccggtgat cgcgtcgccg gcattgaaca gggtgtcgat gcggtcgatg
    52501 gccttaacca gaatatcgtt gatccccacc gggccaccgg tcttcggccg cgcctcggcg
    52561 atctggaaga tcagccgctc ggcctcgtcg agaatctcgg cgccagtacg gccttgtggc
    52621 atataggcgc tgtcggcgat ttcaccgctg atgccgatca actggcgcag cgtggcacgc
    52681 tcacgaatga tctgcgcata ggccttgatg ttggccaccg acggcgtgtt ctttgccagc
    52741 tcaccgagat aggccaagcc accgacttgc gacagctgcc cttcctgatc cagctgctcg
    52801 gagacggtga ccacgtcgaa ggggtggttt cgctcggcca gcttgaaaat cgcgcggaag
    52861 atcaggcggt ggtcatggcg atagaagtcg ccatcggaca ccgcatcgag cacccgctcc
    52921 caggcattgt tgtcgagcat caggccgccg agcacggctt gctcggcctc gatcgagtgc
    52981 ggcggcacct tcagggcaga ggtttgcagg tcgtactgtt cgggcaggct gatgtcggtc
    53041 ataaggctcg gggcaaaatt ctgtgttcac aaaacaaaag gcacgacatc gcttacgcaa
    53101 tgtcgtgcct gattgttagc ggccaggcac ctgagtgcaa gtccacttag tcgcgagcgt
    53161 attaggctgc tactacgaca acccgtacgg ttgcttcaac gtcgctgtgc aggtgcacgg
    53221 ctacgtcata ttcgccgagt tgacgaatgg tgccgttcgg cagacgcact tcagctttgg
    53281 ccacttcaac gccggaggcg gtcagggctt cagcgatgtc gtgagtaccg atggagccga
    53341 acagtttgcc ttcatcgccc gcggtagcgg tgatggtgac ttccagttca gccaactgag
    53401 cagcacggga ttcagcgtac gctttcttct cggcagccag tttttccagc tcggcgcgac
    53461 gcgcttcgaa cgcagcaacg tttgcggcgg tagcagcggt agctttgcct tgcggcagca
    53521 ggaagttacg gccgtaaccg gccttaacgt tcactttatc gcccaggttg cccaggttgg
    53581 cgattttttc cagcaggatc agttccattt ggtaataacc tcttaacttt taaccttcac
    53641 cgttcgcggg gcccgagcct tgcttgcgct caaagcgacc acgaaaatca agcagactgt
    53701 cgacaatggc caaaaccacc agtaacggat aagtcagctg cataaacagc aacagcgtga
    53761 tgtacaaccc gaccagccag aacttaccta gccgaccttg cgccaccatc ccatgcaaca
    53821 gggcgatgcc tgcaaacgct aacggcacac tgcacaacgg cgtcagcatg gccatttgcg
    53881 gacccagatt gggccccaac agcatgccta ccagcagcaa catcgccagc ggagtcggaa
    53941 ggcgtagcgc gcgaaactcg cggccgaaac cacccgggtt atacaactgc gcctgccaga
    54001 agcgcccaag catcaggctc aacaggctga ctatctgcaa caacgccgcg atcaatccgg
    54061 tcagtaccgg tgcaatcagg ctatccagac gcgctcgctc atccaccgac atctgttggt
    54121 gaaccccatc aagcatttgc ggcataagct tttgcagctc aagcgccatt gactcgatgg
    54181 gttcgcggaa caccgcaccc aagaccagcc catacaccag gcccaatgcc acgctgcaca
    54241 gcagcacacg attccaggac aggttggcgc gtaacaacaa cgccaatccc agcgcaccga
    54301 gcaacaccaa cagggtgcgc ggctcaccga aataccacca gccaatggcc ggcagcaggg
    54361 cccaggcgag gatg
//
LOCUS       DASRUM010000193        14091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1101286, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000193 DASRUM010000000
VERSION     DASRUM010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 14091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14091
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101286"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..991
                     /locus_tag="VFF22_12645"
     CDS             5..991
                     /locus_tag="VFF22_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase-like protein"
                     /protein_id="HZX17693.1"
                     /translation="MFGLIKTWKALEAKGIMGINRRNADYVLKYNKRHLYPIVDDKII
                     TKMRAIEAGIDVPEMYGIIDTEKGIDNLRAIIGDRPDFVVKPAQGAGGDGILVIADRF
                     EEKYKTVSGKIISHEEIEHQISSILTGLYSLGGHRDRALIEYRVTPDQIFKSISYEGV
                     PDIRIIVLMGYPVMAMLRLPTRQSGGKANLHQGAIGVGVDLATGVTLRGTWLNNKISK
                     HPDTTNAVDGVQLPNWDGFMKLAAGCYELCGLGYIGVDMVLDQDKGPLILELNARPGL
                     NIQIANDCGLTHRAHAVEARIAELQKKGIVETAEERMRFSQGLFGHVSIAQI"
     gene            complement(1056..2201)
                     /locus_tag="VFF22_12650"
     CDS             complement(1056..2201)
                     /locus_tag="VFF22_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalM family protein"
                     /protein_id="HZX17694.1"
                     /translation="MRIVLMLIGLLVATQTSASGRYLTWVDDQGRVHNSFVDNRYMAH
                     QRTAVGNIQRSDQARLNDDAVIWPGSKSSGESKRRYFTWVDASGNLQNSFYAGQQVQA
                     GGNEILLGTGERSSAYISSDVLEGRDFARDEQDGRYFTWVDEQGRMRNSKVPPKGATP
                     PAPTLHNVALTQGREVTFDAKAQALPGLDGQPTAAMQALLTGTEEKTEGRYQALVERC
                     CGQLNEEDFTPLNADEPRYEELNRFSPSLDFPMGKSYYAALKLPRSQYDFGLRIRSFA
                     NKQVAYPSLLFLDQAKQPTRLVSDAVYQLHAETWYRYAFIEGTVPVRAAAGEHYVLLL
                     TTDEDRSLQTLDNKPFKRPLQALALDEAGMQSHAHSDEGAFELAIVR"
     gene            complement(2211..3803)
                     /locus_tag="VFF22_12655"
     CDS             complement(2211..3803)
                     /locus_tag="VFF22_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009876239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HZX17695.1"
                     /translation="MNTRISLLTQAIFWVASLCSGLAQAQLAQNLTIGSPKAMALGNA
                     VTADFVGIDSVHYNPAALSKLKGRQTTVKFITGVMDIRAEFDAPNGYGSNFLGLNDDP
                     VANSSSRAGAAMMYLPGVGGATELPLLFAPLAGLSITPPGSKLTFATNMYTPQALGFA
                     RDEDDPGRYQGKELVIQRLTYFSPSLAYQVNDELSLGLSIGFSHQAMALNQDFRAPGV
                     LTGLVGVAQDALCLVDNNPFEVFLNICGGELGPFTDIANIDIDMQQTLSPTWNVGFLW
                     EPTDWFALGAVYQSEAKMRLKGRYVVDYSENWQGFWQGLDSSFIGAVFNSITPDGLFD
                     KERGNVSLNMTYPAHFASGIKFKPHERLQINLDVKWTDYAAWDNFELEFDRQLDMLTI
                     ASLFSPDNATSTSIILDRGYESVWSWAVGVEYQVNDRLSLRMGYEPRPSAIPGNKADA
                     LAPLGDANLYGLGLGYQWDKDTVIDLGFNYLVSKQDIPARSSCNINCSSIDSMVYNPY
                     ADLDVKTSVKAYVFALTYRTTF"
     gene            complement(3822..4799)
                     /locus_tag="VFF22_12660"
     CDS             complement(3822..4799)
                     /locus_tag="VFF22_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017516892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HZX17696.1"
                     /translation="MLAGAASSAVFADDPTSVDQAREALSKQEDDADSAKALEEVFQA
                     AEKSYTLLKKGERTLTYGFDYSLLRDTVIETVRTGDNVFSVIGQSEAQHTFTNSFTFD
                     YGVWDNLTFSVRLPMIAKYDTERDLDAYSLGDISASLRWQPWTSLRGKPVTTLYATLG
                     LPTGNSPYDINTQSDLSTGSGTYSLGVGANMSYVIDPVVLFGSVGYTYNMPITGIGQV
                     RGGRLLEEVDPGSSFSLSMGFAYALSYDVSLATSYQMSHSLKPTFTFADGEAEGRGQT
                     SAVMNFSLGLRTSPERIVNVNAGFGMTEDSPDVLLGISMPLDIKGLKAQ"
     gene            complement(4868..5629)
                     /locus_tag="VFF22_12665"
     CDS             complement(4868..5629)
                     /locus_tag="VFF22_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HZX17697.1"
                     /translation="MIEILLGSLIGVWGFTEAVDTKPPAPGTVHINQHLLPNGPLRET
                     VTLEPMSQLQFRNVIRQAYDYSCGSAALTSLLDYFLGRNLQERQVMEGLLQFGEADKI
                     VERRGFSLLDMKRFVTALGYKSGGFRAAFADLDALEHPAIVPIEYAGFKHFVVVRDVY
                     NDHVFVADPALGNISFTRTRFEEIWDQNVLFVIFPNGNEPPNALALSEQDLRIIDDRT
                     VSLLAFREFPQMTKFTENQATELGSDGDIRYIRRK"
     gene            complement(5714..6157)
                     /locus_tag="VFF22_12670"
     CDS             complement(5714..6157)
                     /locus_tag="VFF22_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003152910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6160 family protein"
                     /protein_id="HZX17698.1"
                     /translation="MKTLKQLALAAAVLAVPFMAQAELKAMDDTALATVTGQDGISIS
                     GNFNGTIGSLVYTDGDTGGGSLRMEGIGFTGFNISDDAPVMVDVVTNDISGTATQQLQ
                     ISLPTITGELTVGAIKVGDTNAASIGSLAVSDLNMAGTTVKIWGH"
     gene            complement(6402..9125)
                     /locus_tag="VFF22_12675"
     CDS             complement(6402..9125)
                     /locus_tag="VFF22_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003239860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZX17699.1"
                     /translation="MNSQSNSRTGKLPLLRTKLFPAESAGRPLLAREALLDRLFKARD
                     QHLILLSAPAGFGKSTVLSQFRLRLLDTGARVAWLSCDDADSEPQRLLHYLIAAIEAQ
                     LPGFGASTTRLLRDDLSVPNELLLDSFIADLQKVEGDLYLILDDFHRVRHAELGPLVK
                     YFFKQLPSNVRMIGSTRYQVRFLGDQPVITPKELWVNADELRLTRSETEAYFHELKQL
                     QLSSAELDLLQRRTEGWITALHLVALALARHPERTAFLAGLSGTERNIADYLAEDVLN
                     HLPQDQQQFLDQTSVLDEFNAELCDALTGRTDGAQMLKRLQSAQLFIIALDEQGEWFR
                     YHHLFAEFLQGRLSRVGDPTHLLHAAARWCESHELTDKSIKYALRARDYAFAAELLER
                     QGATLIAGNQVYGILAILKDVPSEVIREHPVFQIFYAWQLAFEQKYAEAEALIEEVST
                     RLMQGRSKPMHFGLAVLLAVAQVLKALVLLYQDKLEAALKVARHWLALVPDNQPIFRA
                     SMSCIQAAAYALLGEFGDAAKSIAVARDNLEQADSEYLHAVASLIEALIFKEMGQPER
                     GRVLAESARARVEQVFGRRSRVGGPLALAYADLLYEQDLHAAILAELPLATIWRDVAT
                     PIELISRGKLVMAKARFFDGEGELALAELDEWLAGLHGPGYERVYALGMSCKVQFLLW
                     LRKPNEAERTCLQLQRHLAVLLPGRYADAETVQVLSEARLALSERRADKAQTVLEACL
                     AKQTAEHQRDRRLRLSLLLSVAYWRKGNSEKAFVLFKATLEDAWASGYRRMFLDDALW
                     LLPLWDAWHGAEPKRANAWQGLAEQLREQCRRLSIDLDNFDENQDVSHREREILRFVA
                     AGLSNRDIAQAVHLSEATIKWHLHNLFAKLGVRSRTQAVLKGKSLGLLSEA"
     gene            complement(9122..11836)
                     /locus_tag="VFF22_12680"
     CDS             complement(9122..11836)
                     /locus_tag="VFF22_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZX17700.1"
                     /translation="MDEVMDLAAGSLLRAKLMPPQVSTDYLVRPQLEAALCANPHARV
                     LLLAAPAGFGKTSALVALAQARECNGQAVAWLSLAPGDDEPSRFLLQLIEAISCLLPG
                     FGAEALAYLQNTMRVPVSAVMESLLVDLAQLEAPLLLVLDDLHLIQDAELISALNRLI
                     KFAPAAFTLAIGSRSQPPLNLATLRAKDLLVEIGEQDLRLSKAEARDYLQRSGLQLDA
                     VAFSALYSQTEGWVVGVHLASLWLRHQPQAAAQIADMGTDQVAVGAYLLRSVFEQLPA
                     DKQELLLALGVAQQLSGDLANALTGRHDGQQLLEELEAMQLFLLPLDRERQWYRFHNL
                     FAEFLRGRLKERDLERFKQLHFNASLWFTNHHMQNLAIEHACLAEDPEMLAALVDGCG
                     LELINRGQLNRIYKWRQHVPDEIAERFPVLVLADVWTRAAELGLVEANRVLDELLERW
                     TEAKSGAPLSDKLLATLAIKSVIALQKDDLQTCIALARRVEVQLGQHTAFLEVAMLIV
                     GALANVMIGQPDQARRLLALAQQRNHFLEGRYLDMQLANVEVLLSLEQGQIRQAELLF
                     AQLRARVIPWFSERSRALALPTITEALIAYNQGRLEGLEERLRWALATVDVINPIDLY
                     AQGMLCMAHVQRMQGNPKDAYASLVLMQNLAARNQSWRFYAQAVADEVAQILQEPAAD
                     RLKRAEQRLKTVDWSKLAEHYQHMRFNPVLWVRGLTRIRLQQARGHYSEALHEITQLR
                     GMLLDNWHGLQRLRLDLLAALSYQRLGYQERAQSLLVQCLIGAEREGVRSLFVEEGEA
                     IRLLLQQLEAAERQPALQGFIRSLLAIWPGNSARQSLEVLEEGLTEREREVVCLAAKG
                     MSNDEIGQQLSLALGTVKWHLHNIYEKLKVRNRTQAIRRARELSLLDQ"
     gene            12000..13349
                     /gene="pabB"
                     /locus_tag="VFF22_12685"
     CDS             12000..13349
                     /gene="pabB"
                     /locus_tag="VFF22_12685"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HZX17701.1"
                     /translation="MPTCLVQALPYHANPSRYFSCVMHAPGAVLLDAGRPAAQRGRYD
                     LISAWPLAEMAPTPDETANAFLQRLRQSLASLGECQAPVENLLPFVGGLIGYLSYDFG
                     RRLEQLPDQAIDDLDLADARFGLYAWALITDHQQQRSQLMFHPRLDFTERQRLGALFA
                     TPAAPAPQPFKLLRRFQSDISEEQYQQCFARIQAYIQAGDCYQVNFAQRFRSDYQGSP
                     WTAYQALRAACPTPFSGYQVLPEGGAIISLSPERFLRISAGQVETRPIKGTRSRHTDP
                     EQDQAQADALLSSQKDRAENLMIVDLLRNDLGRSCRVGSVKVPELFALESYPNVHHLV
                     SAVTGELAADKDALDLIAGSFPGGSITGAPKIRSMQIIDELEPTRRALYCGSLLYLDV
                     RGEMDSSIAIRSLLAKDGQLSCWGGGGIVADSNGQAEYQESITKVKVLLETLEQFCD"
     gene            complement(13480..>14091)
                     /gene="thrH"
                     /locus_tag="VFF22_12690"
     CDS             complement(13480..>14091)
                     /gene="thrH"
                     /locus_tag="VFF22_12690"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HZX17702.1"
                     /translation="IACLDLEGVLVPEIWIAFAEKTGIESLKATTRDIPDYDVLMKQR
                     LRILDEHGLKLSDIQEVIATLKPLEGAVEFVDWLRERFQVVILSDTFYEFSQPLMRQL
                     GFPTLLCHKLITDETDRVVDYQLRQKDPKRQSVIAFKSLYYRVIAAGDSYNDTTMLSE
                     AHAGILFHAPDNVIREFPQFPAVHTYEDLKREFIKASNRELSL"
BASE COUNT         3228 a         4233 c         3818 g         2812 t
ORIGIN      
        1 tgccatgttc ggtctgatca agacgtggaa agccctagaa gccaaaggca tcatgggcat
       61 caatcggcgt aacgcggact acgtgctgaa gtacaacaaa cggcacctgt acccgatcgt
      121 cgatgacaag atcatcacca agatgcgcgc catcgaagcg ggtatcgatg tgccggagat
      181 gtacggcatc atcgataccg aaaaaggcat cgataacctg cgtgcaatca tcggcgaccg
      241 cccggacttc gtggtcaaac cggcgcaggg cgccggcggt gacggcatcc tggtgatcgc
      301 tgaccgtttc gaagaaaagt acaaaaccgt ctcgggcaag atcatcagcc atgaggagat
      361 cgaacatcag atctccagca tcctgacggg cctctactcc ctgggtggcc accgggatcg
      421 tgcgttgatc gaataccgcg taaccccgga ccagatcttc aaaagcatca gctatgaagg
      481 cgtgccagac atccgcatca tcgtgctgat gggctacccg gtgatggcga tgctgcgttt
      541 acccacccgc cagtccggcg gcaaggccaa cctgcaccag ggcgccatcg gcgtgggtgt
      601 ggacttggcc accggggtga ccctgcgcgg cacctggctg aacaacaaga tcagcaaaca
      661 cccggacacc accaacgcgg tggacggtgt gcagctacct aactgggatg gctttatgaa
      721 gctcgccgcc ggctgctatg agctgtgcgg cctgggctat atcggtgtgg acatggtgct
      781 ggaccaggac aagggcccgt tgattctcga actcaacgcc cgccccggcc tgaatatcca
      841 aatcgccaac gactgcggcc tgacccaccg cgcccatgca gttgaagctc gcatagccga
      901 actgcagaaa aaaggcatcg tcgaaacggc tgaagagcgt atgcgcttct cgcaaggact
      961 gtttggccac gtgtcgattg cgcagatctg attgatccga tgactaaaaa atcccggact
     1021 tttccgggat tttttatggc tgctttatgg cgtcgctaac gcacaatcgc cagttcaaac
     1081 gcgccttcat cgctgtgggc gtgactctgc atacccgctt catcgagcgc aagcgcctgc
     1141 aagggacgct taaagggttt gttatccagg gtttgcaggc tgcgatcttc atccgtggtt
     1201 aatagcagta cgtagtgctc accggcggcg gctcgtacgg ggacagtgcc ttcgataaag
     1261 gcatagcggt accaggtttc cgcgtgcagc tggtacaccg catcgctgac caagcgtgtt
     1321 ggctgcttgg cctgatcaag gaacaacaaa gagggatagg cgacctgttt attggcaaag
     1381 ctgcggatac gcaggccgaa gtcatactgc gagcgtggca atttcagggc agcgtaataa
     1441 ctcttgccca tagggaaatc cagactgggg gaaaagcgat tcaactcttc ataacgcggc
     1501 tcatcggcgt tgaggggggt gaaatcctcc tcattcaatt gcccgcagca acgctcgacc
     1561 aacgcctgat agcgcccctc agtcttctct tcggtcccag tcaataaggc ctgcatcgcg
     1621 gccgtgggct gcccatccag tcccggcaag gcttgcgcct tggcatcaaa ggtaacttcc
     1681 cgcccttgag tcagtgccac attgtgcagg gtgggtgctg gcggagtggc ccctttgggc
     1741 ggcaccttag agttacgcat gcgcccttgt tcatcgaccc aagtgaagta gcggccatcc
     1801 tgctcatcac gagcaaaatc gcgcccttcc agtacgtccg agctgatgta ggcactggag
     1861 cgctcacccg tacccagaag aatttcattg ccgccggctt gcacctgctg gcctgcatag
     1921 aagctgttct gcagattgcc actggcgtcc acccaggtga aatagcgacg cttgctctca
     1981 ccgctgctct tactaccagg ccagattact gcatcgtcat tcagacgggc ttggtcgctg
     2041 cgttggatgt tgccaacagc cgtacgctga tgagccatat aacggttatc gacaaagctg
     2101 ttgtgcaccc tgccctgatc atcaacccag gtcagataac ggccgctggc gctggtctgc
     2161 gtagccacta acaaccctat caacatcagc acaatgcgca tgactgaatc ctaaaaagtg
     2221 gtgcggtagg tcaacgcgaa gacataggct ttaacactgg ttttaacatc caggtcggca
     2281 taggggttgt acaccatgct gtcgatggat gagcagttga tattgcagct ggagcgcgcc
     2341 gggatgtcct gcttgctgac caggtagttg aagcccaaat cgatcaccgt gtccttgtcc
     2401 cactggtaac ccagcccgag gccatagaga ttggcatcgc ccagaggtgc cagcgcatca
     2461 gccttattgc cgggaatcgc ggaaggccgt ggttcatagc ccatacgcag gctcaaacgg
     2521 tcattaacct gatactcgac accgaccgcc cagctccata ccgactcata accacggtca
     2581 agaatgatgc tggtcgaagt ggcgttatcc ggggagaaca ggctggcgat ggtcagcata
     2641 tccagctggc gatcaaactc cagctcaaaa ttgtcccagg ccgcatagtc agtccacttc
     2701 acatccaggt tgatctgtaa gcgctcatgt ggcttgaact tgataccgct ggcaaagtgc
     2761 gcagggtagg tcatattcag cgagacgttg cctcgctcct tatcgaacaa accgtccggt
     2821 gttatggagt taaacaccgc gccgataaac gagctgtcca ggccttgcca gaaaccctgc
     2881 cagttctccg agtaatccac tacatagcgc cctttcaagc gcatcttagc ctcgctttga
     2941 tacaccgcac ccaaggcaaa ccagtcggta ggctcccaaa ggaagccgac gttccaggta
     3001 ggcgaaaggg tctgctgcat atcgatatcg atattagcga tgtcggtgaa aggccccaat
     3061 tcgccgccgc agatattcag aaagacttca aacgggttgt tgtcgacaag gcataaggca
     3121 tcctgcgcca ccccgactaa gccggtaaga acccccggcg cgcggaagtc ctgattcagc
     3181 gccatcgcct ggtgcgagaa gccgatagac aagcccagcg acagctcatc gttgacctga
     3241 tacgccagcg aaggcgagaa ataggtcaga cgctgaatca ccagctcctt gccctgatag
     3301 cgccctgggt cgtcttcgtc acgggcaaaa cccagcgctt gcggcgtgta catattggtg
     3361 gcgaaggtca acttggagcc aggcggcgtg atggacaacc cagccaatgg cgcaaacagc
     3421 aacggcaact cggtagcccc ccccacaccc ggcaggtaca tcatggcggc tcctgcgcgg
     3481 ctggatgagt tggccaccgg gtcatcattt aggccgagaa aattgctgcc ataaccattg
     3541 ggggcatcaa actcggcgcg aatgtccatc accccagtga tgaacttgac cgtcgtctgc
     3601 cggcccttga gcttgctcaa ggctgccggg ttgtaatgca cagaatcgat accgacaaag
     3661 tctgccgtta ccgcattgcc tagcgccatt gccttggggc tgccaattgt caaattctgc
     3721 gctagctgtg cctgagcgag cccggagcaa agcgaggcta cccaaaaaat cgcctgcgta
     3781 agcagcgaaa ttcgcgtgtt cataagaaga ttcgctcgcg tttattgggc cttcaggcct
     3841 ttgatatcca aaggcataga gatgcctaac agcacatctg gcgaatcctc ggtcataccg
     3901 aaaccggcgt tgacgttgac gatgcgctcc ggcgatgtac gcaagccgag cgagaagttc
     3961 atcaccgcac tggtctgccc acgcccctca gcttcgccgt cggcgaaggt aaaggtcggc
     4021 ttcagtgaat gcgacatttg ataggacgtt gccagcgaca cgtcataaga cagcgcgtaa
     4081 gcaaagccca tgctcaggga gaatgacgat ccgggatcga cttcttcgag caagcgacca
     4141 ccgcgtacct ggccgatacc ggtgattggc atgttgtagg tgtatccaac agagccgaac
     4201 aacacgaccg ggtcgattac ataagacata ttcgcgccaa cgcccaggct gtatgtgcca
     4261 ctacccgtag acaggtcgct ctgggtattg atgtcataag ggctattacc ggttggcaaa
     4321 cccagggtcg cgtacagggt agtcaccggt ttaccacgta gggatgtcca cggctgccaa
     4381 cgcaggctgg cagagatatc gcccaggctg taagcatcca agtcacgctc ggtgtcgtac
     4441 ttggcgatca tgggcagacg cacgctgaag gtcaggttgt cccacacgcc ataatcgaag
     4501 gtgaacgaat tggtaaaggt atgctgcgct tcactctgac caatcacact gaatacgttg
     4561 tcaccggtgc gtacagtctc gataactgta tcgcgcagca atgagtaatc aaagccatag
     4621 gtcagggttc gctcaccttt tttcagcaag gtgtagcttt tttctgcagc ctgaaaaacc
     4681 tcctccagcg ccttggcgct atcggcgtcg tcttcctgct tgctcaacgc ttcacgtgcc
     4741 tgatcgaccg aagtcggatc gtcggcaaat accgctgaac tggcagcccc tgccagcatg
     4801 gccagccata cacaatgtga aactctcatg gcgcagcccc caaatattta tttttatgcc
     4861 gggcgtttta cttgcgtcgg atgtagcgaa tatcgccatc ggaacccagc tctgtggcct
     4921 ggttctcggt gaatttggtc atctgaggga actcacgaaa tgccagcaaa ctgacggtac
     4981 gatcatcaat gatgcgcaaa tcctgctcgc tcaaggccag ggcattaggt ggttcattgc
     5041 cgttgggaaa gatcacgaac agcacattct gatcccagat ttcctcaaaa cgcgtacgag
     5101 taaaactgat attgccgagt gcagggtccg cgacaaacac atggtcgttg taaacatcac
     5161 gcaccaccac aaagtgtttg aagccggcat attcgatcgg cacgatagcc gggtgctcaa
     5221 gcgcgtcgag atcagcaaag gccgcgcgaa aaccgccact tttgtagccc agcgcagtga
     5281 caaaccgctt catgtcgagc agggaaaagc cgcgccgctc aacaatcttg tcggcctcgc
     5341 caaactgcag caggccttcc attacctggc gttcctgcaa attgcgacca aggaaataat
     5401 ccagcagtga ggtcaaagca gccgagccac agctgtagtc ataggcttga cgaatcacat
     5461 tgcgaaactg caattgactc attggttcaa gcgtcaccgt ctcacgcaaa ggcccgtttg
     5521 gcagcaaatg ctgattgata tgcacggtac ctggcgccgg cggcttggta tccaccgctt
     5581 cggtaaaacc ccacacgccg attaggctgc caagcaaaat ctcgatcatc agcccaccgc
     5641 actcctatcc gacaagaaaa agtcttaccg gccgaaaccg gtaagacttt tcaacacgta
     5701 cgactaagcg aacttagtgg ccccagattt ttacagtggt gccagccatg ttcaggtcac
     5761 taacagccag agaaccgatg ctagcagcgt tggtgtcacc aactttgatg gcacctacag
     5821 tcaactcacc ggtgatggtc ggcaggctga tttgcagttg ctgggttgct gtaccgctga
     5881 tgtcgttggt taccacgtca accatgaccg gagcatcatc agagatgttg aaaccggtga
     5941 agccgatgcc ttccatgcgc agcgaaccac cgccagtgtc gccgtcggta taaaccaggc
     6001 taccgatggt gccgttgaag ttaccggaga tgctgatgcc atcctgaccg gttacggttg
     6061 ccagagcagt gtcatccatg gccttgagct cagcctgagc catgaaagga acagccagaa
     6121 cagcggcggc cagggccagt tgcttgaggg ttttcattgt tattctctcc acagttcact
     6181 aatgggttgt ccccggcagg ctgctctgga ctcctgtccg tctcagctac agcggcgtgc
     6241 gccgctcacc aggtctgata ctgcttccct attcgtacca ggcttggaat cgtgaagcac
     6301 catgcttctc gtcctggtgt taaaactact ggtgcaagcc ttggctgcca gcctatccaa
     6361 gggatggagc gcccacctgg cgcgcatcag gcaaccaggt ctcaggcctc gctcaacaaa
     6421 cccaggctct tgcccttcaa taccgcttgc gtacggctac gcacgcccag cttcgcgaac
     6481 aggttatgca ggtgccattt gatggtggcc tctgacaaat gcaccgcctg ggcaatatcg
     6541 cggttcgaca gcccggccgc tacaaagcgc aaaatctctc gctctctatg gctaacatct
     6601 tgattttcat caaaattatc taaatcgata gacaaccgcc ggcattgctc acgcaattgt
     6661 tcggcaagcc cctgccaagc gtttgcacgc ttgggctcgg ccccatgcca ggcgtcccag
     6721 agcggtagca gccaaagtgc atcatccaga aacatgcgcc ggtaaccgct tgcccaggcg
     6781 tcttccagcg tagccttgaa caagacaaag gccttttcgc tgttaccttt gcgccaatac
     6841 gccacagaaa gtaacaagga aagacgcagg cgccgatcac gctgatgctc agccgtctgt
     6901 ttggccaggc aggcctcaag caccgtttgc gccttatcag cacggcgctc ggacaaggcc
     6961 agacgcgctt cagacagcac ctgcactgtt tctgcatcgg cataacggcc cggtagtaat
     7021 accgccagat ggcgctgcaa ctgcaggcag gtgcgctcgg cctcattggg cttacgcagc
     7081 cacagcagaa actgcacctt gcagctcata cccagtgcgt agacccgctc ataacccggg
     7141 ccatgcaagc cggccagcca ttcatccagc tccgctaggg ccaactcgcc ctcaccgtca
     7201 aagaaacgcg ctttggccat gaccagtttg ccgcgactga tcagttcaat gggcgtggcc
     7261 acatcgcgcc agatggtcgc cagtggcaac tcggcaagga ttgctgcatg cagatcctgc
     7321 tcatacagta aatccgcgta agccaatgcc aacggcccac caacccgact acgccggcca
     7381 aacacctgct cgacccgcgc ccgggcactc tccgctaaaa cgcgaccacg ttctggctgg
     7441 cccatttcct taaagatcaa cgcttcgatc agactggcca ccgcatgcag gtactcactg
     7501 tcagcctgct ccaggttgtc gcgcgccacc gcaatcgatt tggccgcatc accaaactcg
     7561 cccagtagcg cgtaggccgc ggcctgaata caggacatgc tggcacgaaa aatcggctgg
     7621 ttatctggta ctaacgccaa ccagtgacgc gccactttca aggcagcttc caacttgtct
     7681 tgatacagca gtaccaacgc tttcagcacc tgggcaaccg ctaacaacac cgccaggcca
     7741 aagtgcatcg gcttgctacg accttgcatc aagcgcgtgc tgacctcttc aatcagcgct
     7801 tctgcctcgg cgtatttctg ctcgaaagcc agctgccagg catagaagat ctggaacacc
     7861 ggatgttctc gaatcacttc agacggcaca tccttgagga tcgccaaaat gccgtacacc
     7921 tgattgccgg caatcaatgt cgcgccctgg cgttccaaca gctcggcggc aaaggcatag
     7981 tcacgggcgc gcaaggcgta tttgatagac ttatcggtca gctcatggct ttcacaccag
     8041 cgcgcagcag catgcaacag atgggtcgga tcgcccacac ggctgagccg cccctggaga
     8101 aactcagcaa acaggtgatg gtagcgaaac cattcgccct gctcgtccaa cgcgatgata
     8161 aacagctgtg cgctttgcag gcgcttgagc atctgcgcac cgtccgtgcg accggtcagg
     8221 gcatcgcata actcggcatt aaactcatcg agcaccgagg tctgatcgag aaattgctgc
     8281 tgatcctgcg gcaagtggtt cagcacatcc tcagccaagt aatcggcgat attgcgctcg
     8341 gtgccagaaa gtccggccag gaaggcagtg cgctccggat gtcgcgctaa cgccaaagcc
     8401 accagatgca atgcagtaat ccagccctca gtacggcgct gcagcaaatc cagctcggcg
     8461 ctgctcagct gcaattgctt gagctcatgg aaataggctt ccgtctcgct gcgagtgagg
     8521 cgcaattcat ccgcgttgac ccacagctcc ttgggcgtaa tgaccggctg atcaccgaga
     8581 aaacgtactt gatagcgggt actgccgatc attcgcacat tagatggcaa ttgcttgaag
     8641 aagtacttaa ccagcggccc caactccgca tgccttaccc ggtggaagtc atcaaggatc
     8701 aggtacagat caccctcaac cttttgcaga tcagcgataa aggaatccag cagcaactca
     8761 ttgggtacgc tcagatcatc acgcaacagc cgcgtggtac tcgcaccaaa gccagggagc
     8821 tgcgcctcta tggccgcaat taagtaatgc aacaggcgtt gcggctcgct atcggcgtca
     8881 tcacaggaca gccaggccac ccgagcgccg gtatccagca aacgcaggcg aaattggctg
     8941 agcaccgtgc ttttgccgaa accggccggc gcactgagta aaatcagatg ttgatcgcga
     9001 gccttgaaca agcggtcaag cagcgcttca cgggccagca aaggccggcc agcagactca
     9061 gcaggaaaca gcttagtgcg cagtaacggc aacttgcctg tacgggagtt cgattggcta
     9121 ttcattgatc aagaagactc agttcgcgcg cgcggcggat ggcttgtgtg cggttacgca
     9181 ccttgagctt ttcgtaaata ttatgcaggt gccacttaac cgtacccagc gccagtgaaa
     9241 gttgctggcc gatctcatca ttcgacatac ccttagccgc gaggcagacc acctcacgct
     9301 cgcgctcggt cagcccctct tcgagcacct ccagcgattg ccgcgcgcta ttacccggcc
     9361 agatagctag taagctgcgg ataaagccct gaagcgccgg ctggcgttcg gctgcttcca
     9421 actgctgcag caatagacga atagcctcgc cttcttcaac aaacaggctg cgcacaccct
     9481 cgcgctcagc accgatcaaa cattgcacca acaggctttg tgcacgctcc tgatagccca
     9541 ggcgctgata actcagcgcc gccagcaaat cgagtcgcaa gcgctgcaga ccgtgccagt
     9601 tatccagcag catgccgcgc agctgagtaa tttcatgcag cgcctcgctg taatgcccgc
     9661 gtgcctgctg caggcgaatg cgggtcaagc cgcgcaccca cagcaccgga ttgaaacgca
     9721 tatgctggta atgctcggcc agcttgctcc aatccacggt tttgagtcgt tgctctgcac
     9781 gtttcagtcg atccgccgca ggctcttgaa gaatctgcgc cacctcatcg gctaccgcct
     9841 gagcgtagaa acgccaggat tggttgcgcg ccgccaggtt ctgcatcagc accaggctgg
     9901 cgtaagcatc cttggggttg ccctgcatgc gctgtacgtg agccatacac aacatgcctt
     9961 gcgcataaag gtcgatcgga ttgatcacat cgaccgtggc cagcgcccag cgtaagcgct
    10021 cctcgaggcc ttccaggcga ccctggttat aggcgatcag cgcttcagtg atagttggta
    10081 atgccagggc tcgcgagcgc tcggaaaacc aagggatgac tcgcgcgcgt agctgggcga
    10141 acagcagctc ggcctgacgg atctgcccct gctccaggct cagcaacacc tcgacgttgg
    10201 ccagctgcat atccagatag cgcccctcaa gaaaatgatt gcgctgctgc gccagggcca
    10261 gcaagcgcct ggcctgatcc ggctgaccga tcatcacatt agccagcgcc ccgacgatca
    10321 gcattgccac ctccagaaag gcagtgtgct ggcccaactg cacttcaacc cggcgtgcta
    10381 gggcaatgca ggtttgcaaa tcgtctttct gcaaggcgat gactgacttg attgccaggg
    10441 tcgccagcag cttgtcgctc aacggcgcgc cggacttggc ctcagtccaa cgctcgagca
    10501 gttcatcaag cacacggttg gcttccacca gacccagctc ggccgcgcgg gtccagacat
    10561 cggcgagtac caacacggga aagcgctcgg caatttcatc cggcacatgc tgacgccatt
    10621 tatagatccg gttgagctgg ccacggttga tcaactcaag gccacaccca tcgaccaaag
    10681 cggcgagcat ttccgggtct tcggccaggc aggcatgctc gatggccaag ttctgcatat
    10741 ggtggttggt gaaccacagg ctggcattga aatgcagctg cttaaaccgc tccagatcgc
    10801 gctcttttag gcgaccgcgc aaaaattcgg caaacagatt gtgaaaacgg taccactggc
    10861 gttcacggtc cagcggcaag aggaacagct gcatggcttc caactcttcc aacaattgct
    10921 ggccgtcatg ccgcccggtc agggcattgg ccagatcacc actgagctgc tgcgccacgc
    10981 ccagcgccaa tagcaattcc tgtttgtcag ccggcaactg ctcgaacaca ctgcgtagca
    11041 gataagcccc cacagcaacc tggtcagtac ccatatcggc aatttgcgca gcagcctggg
    11101 gttgatggcg tagccaaagg ctggccaggt gcacgccgac cacccagcct tcggtctggc
    11161 tgtacagcgc ggaaaatgcc acagcatcca gctgcaggcc actacgctgc agataatcac
    11221 gggcttccgc cttgctcagg cgcaagtcct gctcgccgat ctcaaccaac aggtccttgg
    11281 cgcgtaatgt ggccaggttt agtggcggct gcgagcggct gccaatggcc agagtaaagg
    11341 ctgcgggggc aaatttgatc agacgattca acgcgctgat caattcagca tcctgaatca
    11401 ggtgcaggtc atcgagcacc agcagcaacg gggcttccag ctgcgctaaa tccaccaaca
    11461 ggctttccat cacggcggag accggtactc gcatggtgtt ttgcaggtag gccagggctt
    11521 cagcgccaaa ccctggcagc aggcaggaaa tcgcctcaat caactgcagc aaaaaacgcg
    11581 acggttcatc gtcaccaggc gcgagcgaca accaggccac tgcctggcca ttgcactccc
    11641 gggcttgtgc caacgcgacc agcgcactgg ttttgccaaa gcctgccgga gcggccaata
    11701 acaacacgcg cgcatgggga ttggcacaca aggccgcctc aagctgcggc ctgaccaaat
    11761 aatccgtcga gacctggggg ggcatcaact tggcgcgcaa aaggctccca gcagcaagat
    11821 ccattacttc atccatggcc ttgatcccgt ggtaattatt gttttatgag tcgcatcgaa
    11881 catcggggga catttccata ctaatgagcg gcagcatccc gagtaaaccc acgcgcaagc
    11941 tctaggatga atgttggcga cgttctacaa tcggcatccg cgtaccttga aagtgtcata
    12001 tgcctacttg tcttgtacag gccctgccct accacgcaaa cccttcgcgt tacttcagct
    12061 gcgtcatgca tgcacccggt gccgtcctgc tggatgcggg gcggccagcc gcgcagcgcg
    12121 gacgctatga ccttatcagc gcctggccat tggcagagat ggcgccgacg cctgacgaaa
    12181 cggccaacgc tttcctgcaa cgcctgcgcc agagccttgc aagcctcggc gagtgccaag
    12241 cgccggtaga gaatctgctc ccgttcgtcg gagggctgat tggctacctg agttacgact
    12301 tcggccgcag gctggaacag ctaccggacc aggccattga cgatctcgac ctagcagatg
    12361 cgcggtttgg cctgtacgcc tgggcgctga ttaccgatca ccagcaacaa cgcagccagt
    12421 tgatgtttca cccacgccta gactttaccg agcgccaacg tctgggggcg ctgtttgcca
    12481 cgccagcagc gcctgcacca caaccgttca agctattgag gcggtttcag tccgatataa
    12541 gtgaagagca gtaccaacag tgctttgcac gcattcaggc ctatatccag gccggtgact
    12601 gctatcaggt taattttgcg caacgttttc gctcagacta tcagggctcg ccatggaccg
    12661 cttaccaagc cttgcgcgct gcctgcccaa cgccattctc aggttatcaa gtgttgccag
    12721 agggcggcgc cattatcagt ttgtcgccgg agcgcttctt gcgcatcagc gcaggccagg
    12781 tggaaacccg accaatcaag ggcacccgct cgcggcatac cgacccagag caggatcagg
    12841 cgcaagccga cgcgctgctc agcagccaga aggaccgcgc ggaaaacctg atgattgtcg
    12901 acctgctgcg caatgacctg ggccgtagct gcagggtcgg ctcggtaaag gtaccggaac
    12961 tcttcgccct ggaaagctac cccaacgtgc atcatctggt cagtgcagtg accggcgagc
    13021 tggcggccga caaggatgcc ctggacctaa tagcaggcag tttccccggc ggctcgatta
    13081 ccggtgcgcc gaagatccgc tccatgcaaa tcatcgacga actggaaccg acccgccgtg
    13141 cgctgtattg cggctcactg ctgtatctgg atgtacgcgg cgaaatggac agctcaatcg
    13201 ctatccgcag cctgcttgcc aaggacggac agctcagttg ctggggcggc ggcggcatcg
    13261 tcgccgactc caacggccag gctgaatacc aggagtcgat taccaaggtc aaagtgctgc
    13321 tggaaacact ggaacagttc tgcgactgac ctggacggca ggcacaaaaa actttcggga
    13381 gaagtttttg acgtcgctca gcgacggccc gaagggtggc cgccatggac acaggccaca
    13441 aaaaagcccg caaatgcggg cttttcaatg gagagctgat tacaggctca gctcgcggtt
    13501 ggacgccttg ataaactcgc gcttgaggtc ttcataggta tgcacagccg ggaactgcgg
    13561 gaattcgcga atcacgttat ccggcgcatg gaacaggata ccggcgtggg cttcgctgag
    13621 catggtggtg tcgttgtacg agtcacccgc cgcaataacg cgatagtaaa ggctcttgaa
    13681 ggcgatgacc gactgacgct tggggtcctt ctggcgcaat tggtaatcca ctacccgatc
    13741 cgtttcatcg gtgatcagct tgtggcatag cagagtcggg aagcccagct gacgcatcag
    13801 cggctgggaa aactcgtaga aggtgtccga gagaatcacc acctgaaagc gctcacgcag
    13861 ccagtcaacg aactccaccg caccttccag aggcttgagt gtggcgatca cttcctggat
    13921 atcggacagt ttcaagccgt gctcatccag gatgcgcagg cgctgcttca tcagcacgtc
    13981 atagtccgga atatcccggg tggtcgcttt cagcgattcg atgccagttt tttccgcgaa
    14041 ggcgatccag atttccggga ccaacacgcc ttccaggtcg agacacgcga t
//
LOCUS       DASRUM010000194         8067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2116788, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000194 DASRUM010000000
VERSION     DASRUM010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2116788"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..877)
                     /locus_tag="VFF22_12695"
     CDS             complement(176..877)
                     /locus_tag="VFF22_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017278840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HZX17703.1"
                     /translation="MHDTPSRAVPEYAVQAIHQLIDAGGYRPGDTLPSQRDLAEQLGV
                     SRASLREALSTLSALGRVSVQPGKGVFVREPPMSSSSAFAWPFAAQVSAADTFQLRFA
                     LEGFACGLAAATLTAAELDALQDNVEQMRLELRGGDFESASRLDFDFHRRILLASGNQ
                     AMCGIVTSSADIFLESQKLPFIRPERAMETWQEHRKILKALGRHASGPAQKAMQQHIR
                     AAALRTGIAFITLGD"
     gene            1156..1941
                     /locus_tag="VFF22_12700"
     CDS             1156..1941
                     /locus_tag="VFF22_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX17704.1"
                     /translation="MTLTPRRLLTALFASLAIGVSPAFADGLADITERGVLKVAVPQD
                     FPPFGSVGPDMKPRGLDIDAAQLLADQLAVKLELTPVNSTNRIPFLTTGKVDLVISSL
                     GKNPEREAVIDFSAAYAPFYLGVFGPEDATISSLDDLDGKTISVTRGSIEDIELSKVA
                     PKGATLKRFEDNNSTIAAYLSNQVDLIASGNVVMVAIAERNPKRVPAMKIKLKDSPVY
                     VGVNKNETALLEKVNAILSAGKADGTLSAISEKWLKQPLPADL"
     gene            1967..2632
                     /locus_tag="VFF22_12705"
     CDS             1967..2632
                     /locus_tag="VFF22_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003301067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZX17705.1"
                     /translation="MAYQFDFIPVLANADLLLQGAFFTLSLTAIGTLLGVGLGIIGAV
                     MRAWRIKPFSQLFGLYVELIRNTPFIVQLFFIFFGLPALGVRMNEWEAAVLAMVINLG
                     AYSTEIIRAGIQAIPSGQLEAAAALAMNRIEIFRHVVLRPALAKVWPALSSQIIIVML
                     GSAVCSQISTEELSFAANFIQSRNFRAFETYLVTTLLYLAMAILIRQLLAWVGRRFVV
                     GGR"
     gene            2629..3279
                     /locus_tag="VFF22_12710"
     CDS             2629..3279
                     /locus_tag="VFF22_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020797254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZX17706.1"
                     /translation="MMDFTFWDILRNLLVGLQWTLLLSLVAFACGGVAGLLLLLARIS
                     PLQPLRVLARGYIELFQGTPLLMQLFMVFFGIALLGIDVSPWLAAACALTLFTSAFLA
                     EIWRGCVESISHGQWEAAESLAMSRLEVLRHVVLPQAMRIAIAPTVGFSVQVVKGTAV
                     TSIIGFTELTKTGGMLANATFEPFMVYGLVALGYFLLCYPLSLAAYRLERRLNVTA"
     gene            3266..3997
                     /locus_tag="VFF22_12715"
     CDS             3266..3997
                     /locus_tag="VFF22_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZX17707.1"
                     /translation="MSLLNVTALHKYYGDNHVLKGIDLKVEEGEVVAIIGRSGSGKST
                     LLRTLNGLESINDGVIEVDGDYIDAARADLRSLRQKVGMVFQQFNLFPHLSVGENIML
                     APQVVKKMGRDDAQQLARQMLERVGLGEKFDAYPERLSGGQQQRVAIARALAMSPRVL
                     LCDEITSALDPELVNEVLAVVKQLASEGMTLIMVTHEMRFAREVGDKLVFMHQGKVHE
                     VGDPKQLFAEPQTAELRQFIGSINF"
     gene            4301..5803
                     /gene="ppx"
                     /locus_tag="VFF22_12720"
     CDS             4301..5803
                     /gene="ppx"
                     /locus_tag="VFF22_12720"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HZX17708.1"
                     /translation="MPHTPAESFPLIAAIDLGSNSFHMVLAKADHGEIRILERLGDKV
                     QLAAGIDEQRLLSEEAMQRGLDCLRRFAQLTASLPEGAVRVVGTNALREARNRGEFIR
                     RAEEILGHPVEVISGREEARLIYLGVSHSIADTPGKRLVADIGGGSTEFIIGQRFEPL
                     LRESLQMGCVSFTQRFFKDGKITPARYAQAYTAARLEIMGIEHALRRLGWEDAVGASG
                     TIKAIGLTIQAAGLGTGEVNAQGLAWLKRKVFKAGEVEKLDLDGIKPDRRGIFPAGLA
                     ILEAIFDACDIQRMSHSEGALREGVLYDLLGRHQHEDVRERTLSSLMERYHVDLEQAA
                     RVESKALSALEKVANDWQLEDDWHRELLSWAAKVHEVGLDIAHYQYHKHGAYLVEHSD
                     LSGFSRQDQQMLALLIRGHRRNIPKDKFADFGAEGVKLIRLCVLLRFAILFHHIRGTQ
                     EMPNVQLKAAGQRLEIQFPAGWLENNPLTQADFTQEAEWLKRIGIELSVS"
     gene            complement(5821..8025)
                     /gene="ppk1"
                     /locus_tag="VFF22_12725"
     CDS             complement(5821..8025)
                     /gene="ppk1"
                     /locus_tag="VFF22_12725"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009046131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HZX17709.1"
                     /translation="MNTEGLSTPELQDAQPVVDEVVAVEVPVIEAPVPVAPAPVVIHN
                     LDDSSLYIHRELSQLQFNIRVLEQALDESYPLLERLKFLLIFSSNLDEFFEIRVAGLK
                     KQINFAREQAGADGLQPHQALARIAELVHEQVDRQYAILNDNLFPALAKHNVNFIRRR
                     FWTAKHKAWVRRYFRDEIAPIITPIGLDPTHPFPLLVNKSLNFIVELEGIDAFGRDSG
                     LAIIPAPRLLPRIIKVPEEVGGPGDNFVFLSSMIHAHADDLFQGMKVKGCYQFRLTRN
                     ADLSVDTEDVEDLARALRGELFSRRYGDAVRLEVVDTCPRPLLDYLLKQFSLTEAELY
                     RVNGPVNLTRLFSITGLESHPELQYKPFTPVIPKLLQNAENIFSVVSKQDILLLHPFE
                     SFTPVVDLLRQAAKDPHVLAIKQTLYRSGANSEIVDALVEAARNGKEVTAVIELRARF
                     DEESNLQLASRLQAAGAVVIYGVVGFKTHAKMMLILRRESGDIVRYAHLGTGNYHAGN
                     AKLYTDYSLLTADMALCEDVSKLFSQLIGMGKTLRMKKLLHAPFTLKKTLLDMIARET
                     ALAAEGKPAHIIAKFNSLTDPKIIRALYKASQTGVKIDLVVRGMCCLRPGIAGVSHNI
                     QVRSIIGRFLEHTRVFYFSNGGEEKIYLSSADWMERNLDKRVETCFPVEGKKLILRVK
                     KELESYLTDNTQAWVLQSDGRYLRTSPSGNQNPRSAQAGLLEKLTVPLVIAR"
BASE COUNT         1545 a         2558 c         2439 g         1525 t
ORIGIN      
        1 cctggagaac atcctggtcg aaactggcat cgctgacatt ggtgatgaat tcgctcatgg
       61 aaagtctccg agttcgtaag caaaagatgg gcgacatcat agcccggctt ttcccacaca
      121 ggaaggcggc ggcaattgag cctaactatg ggccttggaa agaagttgcc cggcgctagt
      181 cgccgagggt gataaatgcg atgccagtac gcagggccgc cgcgcgaata tgctgctgca
      241 tggccttctg cgccgggccc gaggcatgcc gccccagcgc cttgaggatc ttgcggtgtt
      301 cctgccaggt ctccatcgcg cgctctgggc ggatgaacgg tagcttctga ctttccagaa
      361 aaatatccgc gctggaggtg acgataccgc acatggcctg gttgccgctg gccagcagga
      421 tgcgccggtg aaagtcgaaa tccaggcgtg aagcgctctc gaaatcaccg ccgcgcagct
      481 ccaggcgcat ttgctcgaca ttgtcctgca gggcatccaa ctcggcggcg gtcagggtgg
      541 ctgccgccag gccacaggcg aaaccttcca gggcaaaacg cagctgaaag gtatcggctg
      601 cactgacctg ggcggcgaag ggccaggcaa acgcgctgct agaggacatc ggcggctcgc
      661 gcacgaacac ccccttgccc ggctgtacgc tgacccgccc caaggcactg agggtcgaca
      721 gtgcttcgcg cagcgaagcc cggctaacgc ccaactgctc agccagatcc cgttgcgaag
      781 gcaaggtatc gccagggcga tagccgcctg cgtcgatcaa ctgatggatc gcttggacgg
      841 catattcggg taccgcgcgg gagggtgtgt catgcatcac tgttcaggcc ggtctgtccg
      901 taagagcgcc ctttgtaagg cctgaagcac actccaggca agcactgcac cctcacggat
      961 caacaccaca cccaatcgca ccaagaaagc gcacacggca ctatgaccaa ccagtcaaga
     1021 tcgccatctg ttcagaccag taagaccttg taattactcg cagacgccga taatcaacag
     1081 tgaatacgcg actttggcat ggtggatgca acatccaaga caacgcattc cgcatatttt
     1141 gctcaggagt ttgccatgac cctcaccccg cgccgcctgt tgaccgcctt gtttgccagc
     1201 ctcgccatcg gtgtcagccc ggcctttgcc gacggcctcg ccgatatcac cgaacgcggc
     1261 gtcttgaagg tcgcggtacc ccaggatttc ccaccattcg gctcggtcgg tccggacatg
     1321 aaaccacgcg ggctggatat cgatgccgcc cagctactgg ccgaccaact ggcggtgaaa
     1381 ctggagctga ctccggttaa cagcaccaac cgcatcccct tcctcaccac tggcaaggtc
     1441 gacctggtga tttccagcct gggtaaaaac cctgagcgcg aagcggttat cgacttctcc
     1501 gccgcctacg cgcccttcta tctcggcgta ttcggcccgg aagatgccac gatcagcagc
     1561 cttgatgacc tggatggcaa gaccatcagc gtcacccgcg gctcaatcga agacatcgag
     1621 ctgagcaagg tcgcccccaa aggcgccacc ctcaagcgct tcgaagacaa caactcgacc
     1681 atcgccgctt acctttccaa tcaggttgac ctgatcgcca gcggcaacgt ggttatggtg
     1741 gccatcgccg agcgcaaccc caagcgcgtg ccagcaatga agatcaagct gaaagactcc
     1801 ccggtctatg tcggcgtaaa caagaacgaa acagccctac tggagaaggt caacgccatc
     1861 ctcagcgcag gcaaggccga tggcaccctc agcgccatca gcgagaaatg gctgaagcaa
     1921 ccgctgccag cagacctgta agaggcgcag agacaggagc cgcgcgatgg cgtaccagtt
     1981 cgattttatc ccggtgttgg ccaacgccga cctgttgctg caaggcgcat tcttcaccct
     2041 cagcctgact gccatcggca ccctactggg ggtcggtcta ggcatcatcg gtgcagtgat
     2101 gcgcgcctgg cggatcaagc cgttcagcca gctgtttggc ctgtatgtcg aactgatccg
     2161 caacacgccc tttatcgtcc agctgttctt tatcttcttc ggcctgccgg ctctgggcgt
     2221 gcggatgaac gagtgggagg cggcggtgct ggcgatggtg atcaacctgg gggcctactc
     2281 caccgagatc atccgcgccg gcattcaggc catccccagc ggccaactgg aagccgctgc
     2341 ggccctggcc atgaaccgca tcgagatttt ccgccacgtg gtcctacgcc cggccctggc
     2401 caaggtctgg ccggcgctgt cgagccagat catcatcgtc atgctcggct cggcggtgtg
     2461 ctcgcagatt tccaccgagg agctgtcgtt cgccgccaat tttatccagt cgcgcaactt
     2521 ccgcgccttt gaaacctacc tggtgaccac cctgctctat ctggccatgg cgatcctgat
     2581 ccgccagctg ctggcctggg tcggccgccg cttcgtcgtc ggaggccgct gatggacttc
     2641 accttctggg acatcctacg caacctgctg gtgggcctgc agtggacgct gctgctgtcg
     2701 ctggtggcct tcgcctgcgg cggcgtggcc gggctgctgc ttctgcttgc gcggatttca
     2761 ccgctgcaac cgctgcgggt tctggcacgt ggctatatcg agctgttcca gggcacgccg
     2821 ctgctgatgc agctgtttat ggtgttcttc ggcatcgccc tgctcggtat cgacgtatcg
     2881 ccctggctgg ccgccgcctg cgccctgact ctgtttacca gcgcctttct cgccgagatc
     2941 tggcgcggct gcgtcgaatc gatcagccac ggccagtggg aagccgccga aagcctggcc
     3001 atgagccgcc tcgaagtgct gcgccatgtg gtgctgccac aggccatgcg catcgccatc
     3061 gcccccaccg tgggcttctc ggtgcaggtt gtgaaaggca ccgcggtgac ctcgatcatc
     3121 ggttttaccg aactgaccaa aaccggcggc atgctggcca acgccacctt cgagcccttc
     3181 atggtctacg gcctggtggc gctcggctac ttcctgctct gctaccccct gtcgcttgcc
     3241 gcataccgcc tggaaaggag gctgaatgtc actgcttaac gtcaccgccc tgcacaaata
     3301 ctacggcgac aaccatgtgc tgaagggcat cgatctgaag gtcgaggaag gcgaagtggt
     3361 cgccatcatc ggccgcagcg gctcgggcaa gagcaccctg ctgcgcaccc tcaacggcct
     3421 ggaatcgatc aacgacggcg ttatcgaggt cgatggcgac tatatcgacg ccgcccgtgc
     3481 ggacctgcgc agtctgcggc agaaggtcgg catggtattt cagcagttca acctgttccc
     3541 ccatctgagc gttggcgaga acatcatgct ggccccccag gtggtgaaga agatgggccg
     3601 cgacgacgcg cagcaactgg cgcggcagat gctcgaacgg gtcggcctgg gcgagaagtt
     3661 cgacgcttac ccggagcgtt tgtccggcgg ccagcaacag cgggtggcca tcgcccgcgc
     3721 cctggcgatg tcgccgcggg tgctgttgtg cgacgagatc acctcggcgc tcgacccgga
     3781 gctggtcaac gaagtgctgg cggtggtcaa gcaactggcc agcgagggca tgaccttgat
     3841 catggtcacc cacgagatgc gcttcgcccg cgaggtcggc gacaagctgg tgtttatgca
     3901 ccagggcaag gtgcatgaag tgggcgatcc caagcagcta ttcgccgaac cgcagacggc
     3961 ggaactgcgc cagtttatcg gctcgatcaa cttctaagca cgcctactac ccggcggcta
     4021 atgcgtgccg cactgatcgc cagtcagcga actcgacgaa cgggcgattc gggcgtttat
     4081 cagcagcccg cagccgggag gatttgagtc tgcagcgccc ggtgcgcgcg gttgtgatgg
     4141 gtcacggcaa cccggcaccc aacagccctt ggcctctcct gcaacagccc cggtgggcct
     4201 gtttgcagag cctgccgcag ggtatgcgac atcagtatgc gttggcgggg cctggctatc
     4261 gtggcacgat ggcgcggttc cgtattgaga tggttctgcc atgccgcata cccctgccga
     4321 gtcttttcct ttgattgcag ctatcgacct gggctccaac agcttccata tggtgctggc
     4381 caaagccgac cacggcgaga tccgcatcct cgaacgcctc ggcgacaagg tccaactggc
     4441 cgccggcatc gacgagcagc gcctgctcag cgaagaagcc atgcaacgcg ggctcgactg
     4501 cctgcgccgc ttcgcccagc tgaccgccag cctgccggag ggcgcggtgc gcgtggtggg
     4561 caccaatgcc ctgcgcgagg cacgcaaccg tggcgaattt atccgccgcg ccgaagagat
     4621 cctcggccac ccggttgaag ttatctccgg gcgcgaagaa gcacgactga tctatctcgg
     4681 tgtatcgcac agcatcgccg acactcccgg caagcgcctg gtcgccgata ttggcggcgg
     4741 cagcaccgag ttcatcatcg gccagcgttt cgagccgctg ctgcgcgaaa gcctgcagat
     4801 ggggtgcgtc agctttaccc agcgtttttt caaggacggc aagatcaccc cggcgcgtta
     4861 cgcccaggcc tacacggcgg cgcggctgga gatcatgggc atcgagcacg ccctgcgtcg
     4921 cctgggttgg gaggatgccg tgggcgcatc cggcaccatc aaggccatcg gcctgaccat
     4981 tcaagcagcc ggcctgggca ccggcgaagt caacgcccag ggcttagctt ggctgaaacg
     5041 caaagtattc aaggccggtg aggtggaaaa actcgacctc gacggcatca aaccggatcg
     5101 ccgagggatt ttcccggctg gcctggcgat tctcgaagcg atcttcgatg cctgcgatat
     5161 ccagcgcatg agccattccg aaggcgcgct gcgcgaaggc gtactgtatg acctactcgg
     5221 ccgccatcag catgaagacg tgcgtgaacg caccctgagc tcgctgatgg agcgctatca
     5281 cgtcgatctg gagcaggctg cgcgggtcga gagcaaggcg ctctcggcac tggagaaggt
     5341 ggccaacgac tggcaactgg aagatgactg gcaccgtgaa ctgctcagtt gggcggccaa
     5401 ggtgcacgaa gtcggcctgg acatcgccca ttaccagtac cacaagcacg gcgcctacct
     5461 ggtcgagcac tcggaccttt ccggcttctc gcgtcaggat cagcaaatgc tcgccctgtt
     5521 gatacgcggc catcggcgca acatccccaa ggacaagttc gccgacttcg gcgctgaagg
     5581 cgtcaagctg atccgcctgt gcgtgctgct gcgctttgcc atcctgttcc accacatccg
     5641 tggcacccag gaaatgccca acgtgcagct caaggccgcc gggcagcgcc tggagattca
     5701 gttccccgca ggctggctgg agaacaaccc gctgactcag gccgatttta cccaggaagc
     5761 ggagtggctc aagcgtatcg ggatcgaact gagcgtgagc tgacgctcac gcccccctgc
     5821 ttatcgtgcg atgaccaacg gcacggtgag tttctccaac aacccggcct gcgcactgcg
     5881 cgggttctgg ttgccgctcg ggctggtacg caggtagcgc ccatctgact gcagcaccca
     5941 ggcctgggtg ttgtcggtca ggtagctttc cagctccttt ttgacccgca aaatcagctt
     6001 cttgccttcc accgggaagc aggtctcgac gcgcttgtcg aggttgcgct ccatccagtc
     6061 agcactcgac aggtagatct tctcctcgcc gccattggag aaatagaaca cccgcgtgtg
     6121 ctccaggaag cgacctatga tcgagcgcac ctggatattg tgcgaaaccc cagcaatccc
     6181 cggacgcagg cagcacatgc cgcgcaccac caggtcgatc ttcaccccag tctggctcgc
     6241 cttgtacagc gcgcggatga tcttcggatc ggtcagcgag ttgaacttgg cgatgatatg
     6301 cgccggcttg ccctcggccg ccagtgcggt ttcgcgggcg atcatgtcga gcagggtctt
     6361 tttcagggta aacggcgcgt gcagcagctt cttcatgcgc agggtcttac ccatgccgat
     6421 cagctgactg aacagcttgg agacgtcctc gcacagcgcc atatcggcgg tgagcaggct
     6481 gtagtcggtg tacagcttgg cattgccggc gtggtagtta ccggtgccga ggtgggcata
     6541 gcggacgata tcgccactct cgcggcgcag aatcagcatc atcttggcgt gggttttgaa
     6601 ccccaccacg ccgtagatca ccaccgcgcc ggccgcctgc agacggctgg ccagctgcag
     6661 gttggactct tcatcgaaac gcgcacgcag ttcgatcacc gccgtgactt ccttgccgtt
     6721 acgcgcggct tccaccagcg cgtcgacgat ttccgagttg gccccggagc ggtacagggt
     6781 ctgcttgatc gccagcacgt gaggatcttt cgccgcctga cgcagcagat cgaccaccgg
     6841 ggtaaaagac tcgaagggat gcagcaggag gatgtcctgc ttgctgacca cgctgaaaat
     6901 gttctcggcg ttctgcaaca gcttggggat caccggcgtg aagggcttgt actgcagctc
     6961 cgggtggctt tccaggccgg tgatgctgaa caggcgggtc aggttgaccg ggccattgac
     7021 ccggtacaac tcggcctcgg tcaggctgaa ctgcttgagc aggtaatcca acagcggccg
     7081 tgggcaggtg tcgaccactt ccaggcgcac cgcatcgcca taacggcgac tgaacagctc
     7141 gccacgcaga gcgcgggcca ggtcttcgac gtcctcggtg tctaccgaga ggtcggcgtt
     7201 acgggtcagg cggaactggt agcagccctt gaccttcatg ccctggaaca ggtcatcggc
     7261 gtgggcgtgg atcatcgacg acaagaacac gaagttgtcg ccaggcccgc ccacctcttc
     7321 cggcaccttg atgatgcgcg gcagcagacg cggcgctgga atgatcgcca gaccggagtc
     7381 gcggccgaag gcatcaatac cttctagctc gacgataaag ttcaggctct tgttcaccag
     7441 cagcgggaac gggtgcgtcg ggtcgaggcc gatcggggtg atgatcggcg cgatctcgtc
     7501 gcggaaatag cggcgtaccc aggccttgtg cttggccgtc caaaagcgcc gacggataaa
     7561 gttgacgttg tgcttggcca gcgctgggaa caggttgtcg ttgagaatcg cgtactgccg
     7621 atccacctgc tcgtgcacca gctcggcgat ccgcgccagc gcctggtgcg gctgcaggcc
     7681 atcggcaccg gcctgttcac gggcgaaatt gatctgcttc ttcagcccgg cgacgcggat
     7741 ctcgaaaaac tcatccaggt tgctggagaa aatcagcaga aacttcagcc gctccaacaa
     7801 cggataggac tcatccagcg cctgctctag cacgcggata ttgaattgca gctgcgacag
     7861 ctcgcggtgg atatacagac tgctgtcatc caggttatgg atcaccacag gcgccggagc
     7921 gaccggcact ggggcttcga tgacaggcac ttccacggct acaacctcgt cgacgaccgg
     7981 ctgggcgtct tgcaattcag gggtactgag tccttcggtg ttcattacaa agtcctggcg
     8041 gggctattgc ccctgcttca gcagttc
//
LOCUS       DASRUM010000195        35633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_436823, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000195 DASRUM010000000
VERSION     DASRUM010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 35633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35633
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436823"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1309)
                     /locus_tag="VFF22_12730"
     CDS             complement(83..1309)
                     /locus_tag="VFF22_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HZX17710.1"
                     /translation="MPDTQLTTRPRNLSRQWLLGSLALAVLAVLLWWFWPSPPEAPQR
                     GGPWRDGGPVPVRVATVSQGDFPVELKALGTVTAYNTVNVRGRVDGELVKLLFKDGQQ
                     VKAGDLLAVIDPRSYEIALQQAQGALQEHQAQLRNAELDLARYRGLYAEDSIAKQTLD
                     TQEALVNQFRGSLKSLQADVATARLNLDFTQIRAPITGRLGIRQADVGNLVSSGDELP
                     LVVITQTLPIAVMFTLPEAELPAVVQQLRSGATLAVEAWDRSEKLKLAEGELESVDNL
                     IDTATGTVKLKARFANAEEMLFPNQFVNVRLRVKTLQNVTLMPAAALQFGASGTFAYV
                     VDAEDKVQLTPISIGASDGQLSVVEQGLKAGDRLVLEGTDRLRSGNKVEVIGDTPVAA
                     QSAPATPESKRAPRAQ"
     gene            complement(1502..2002)
                     /gene="tpx"
                     /locus_tag="VFF22_12735"
     CDS             complement(1502..2002)
                     /gene="tpx"
                     /locus_tag="VFF22_12735"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003447601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HZX17711.1"
                     /translation="MAQVTLKGSPVEVTGQLPQIGQQAPAFSLVAGDLSDVSLASLAG
                     KRKVLNIFPSVDTPTCATSVRTFNTSASQLANTVVLCISTDLPFAQARFCGAEGLENV
                     INLSTMRGADFLQNYGVAIASGPLVGVAARAVVVLDENDKVLHSELVGEIADEPNYDA
                     AIAVLK"
     gene            complement(2145..3488)
                     /locus_tag="VFF22_12740"
     CDS             complement(2145..3488)
                     /locus_tag="VFF22_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HZX17712.1"
                     /translation="MEFVNTLVNQLNGVVWGPPMLVLILGTGLFLMLRLKFMPLSKIG
                     AGFKLMWQGRQKGDAESGEISPFQALMTCLAATVGTGNIAGVATAIFLGGPGALFWMW
                     CTALVGMATKYCEVVLAVHYREKDERGEHVGGPMYAIKNGLGKKWLWLGTAFAIFGGF
                     AGFGIGNMVQVNSMAAALQTTFNIPLWVTGVVTMVIVGMVILGGIKRIGKVAATLVPF
                     MCVAYIIAAMVVLVVNASEIPKAFEMIFTYAFTPIAATGGFAGAAVMAAIRFGVARGI
                     FSNEAGLGTAGIAQAAGTTNSPVRSGMIGMLGTFIDTIIICSMTGLAIITSGVWTSGA
                     SGAALSAAAFEQAMPGVGGYILTLALVVFAFTTILGWSYFGEKCWEYMVGTKSILPFR
                     ILWVVAVPFGAIAQLDFAWLVADTLNGLMAIPNLLSLLLLSPIVVKLTKDHFAKN"
     gene            complement(3783..7451)
                     /locus_tag="VFF22_12745"
     CDS             complement(3783..7451)
                     /locus_tag="VFF22_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009049807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HZX17713.1"
                     /translation="MKRGLRYGLFSLLGLLLVLAISLSLLLGTQGGSRWLLAQVPGLQ
                     VDDFNGRLGSGWSATQLRWQQGENSVQVMAPSLDWSPACLLRLTLCIEQLHSGPISLV
                     FPEDGQPSSEPFSLPDLSLPLALQLGQVRIDSLQLNGVEQLQGLQLAARWTVQGIQID
                     NLSLRSADLQVALQGKVQTSGNWPLQLSGQLQLPAPEHQAWPVALQLEGELRERVQVT
                     ASSSGYLQGELTGWLQPLAEHLPAQLRLQVAAFKASAELPDTSNLEQVELNAQGDLQD
                     GYQVTGSASLPATEGPIALALAGQLKADGATISSLSLTAEPEQQVNVSGELSWQQGLS
                     LDSRFNWQNFPWQRLYPLDEPAPVALRMLDGELAYQDGNYLGHFAAELDGPAGAFSLQ
                     SPLSGDLQNLYLPELRLQAGQGRAEGQVSLGFAEQFSWDVRLQLSQLDPAYWLAELPG
                     SLAGPLNSSGSLINQQLNLNADIDLTGRLRGQPAKLLAKFAGGLEQGELSALDVRLGD
                     NRISGKAALRQQLNGQLLLAMPRLGQLWPGLQGQLNGQLDLAGSLQAPQGQLQLQGQR
                     LAFDDQRLQQLKLDASLNSAQRGRITLDAQGIQLGETDLGHLTANAAGNQQQQSLELQ
                     LQGPLLQSQLALAGKLDKGNWRGSLSRGEVQSGGQDWRLQQPATLVRLANGQLSLGAH
                     CWRSGGASLCGTEQRLLPQPKLNYQLSNFPMESLAALWPKDFAWQGELNGRLQLDLPA
                     SGPNGSLELDAGIGIWRIRDQQEWLDFAYDSLRLSAQLRPQRIDSLLELRGPAIGELS
                     LQAQLDPRTASKPLSGSFRLNGLDLALARPFVPMVERIAGQLNGSGTLSGGLLAPQIN
                     GNLRVVNGEISGGELPMNIEQLQLQARIEGERLQLEGDWRSGEQGQGSLNGELSWTDG
                     LLADMQLRGSRLPVTVEPYAALEVEPDLRLSLRDEQLSVAGKVLIPSGKIEVRELPPS
                     TVQVSSDAQVVGHESTQRQATAIAMDIEVEVGQQRLTFSGFGLDAELLGRVHIGNDLD
                     TRGELSLNKGRFRAYGQRLTVRRARLLFAGPIDQPFLDIEAVRQVDDVTAGLRLSGNA
                     AQPTSEVFSEPAMSQEQALSYLIMGRPLGQGGGDNNMLAQAALALGMAGSAPLASSLA
                     QGLGISDFQLDTQGSGVTTSVVASGNLSERLSLRYGVGVFEPANTIALRYELSRRLYL
                     EAASGLASSLDLFYRRDF"
     gene            complement(7448..9175)
                     /locus_tag="VFF22_12750"
     CDS             complement(7448..9175)
                     /locus_tag="VFF22_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HZX17714.1"
                     /translation="MPVSPHLFTALFLLLLSATALAEAQLRVRVEPADSALKSNIEGY
                     IGSLGDRDAEALQRFRRVAESQAEKAAQALGYYQAQITTEVSESATPRLTLRIQPGEP
                     VRLRNVLVRIDGPAAQLRDFRTPNDQQLRSGAQLNHGRYDAAKRDIQNQASRYGFFAG
                     RFSSQRLTIDPEAGVADIELVYVSGPRYQLGAVSFVGDAPFDEQLLQRMVPFPADTPY
                     DSALIAELYQALRSSGYFESVQVDANPASANAEQIPVQVQLQARKPRSMGLGLGFSTD
                     VGPRGRANWTRHWANPQGHSYGAEFELSAPRQNVGLWYDVPLDPPLTDKLRIAGGYQA
                     EDLANTDSSSRLLTFGPEWHSKLDSGWQRVLLLKWQHDEYDLGDDAGTSTLLMPGLSY
                     SYLQSDNQIDPNHGYRLQFDLSGAAKGLLSDANVLHGNVQLKGLTTLFTRHRLLGRVQ
                     VGGTESDGFAKVPPSLRFFAGGDQSVRGYDYQSLSPENRAGDKIGGRYLFTSSLEYQY
                     SLTERWRLASFFDQGNTFDSLEIPSLKSAVGLGVRWVSPLGPLRLDLAHPLDDQGGVR
                     LHFSMGPEL"
     gene            complement(9276..9917)
                     /locus_tag="VFF22_12755"
     CDS             complement(9276..9917)
                     /locus_tag="VFF22_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX17715.1"
                     /translation="MPNSLAEVRMLDGGYAREARSLLYHAYRHDPTFAYLFEAERPGY
                     DQRVRATVRELVQQHFSEDLPAIGLLIDDRLIGMALIAPPQRRMDITESWGWRMRMLL
                     TTGFRCTKRYLEYHDAVIACLPPGPYHVLPLLGIHPEYQGKHLGEQLLEALHNWCAED
                     ASSQGVVLDTGNSHYLDFYKRQGYEEIGEVAIGPIIEHVFFHPSPQVAIKASA"
     gene            10061..10876
                     /gene="xthA"
                     /locus_tag="VFF22_12760"
     CDS             10061..10876
                     /gene="xthA"
                     /locus_tag="VFF22_12760"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HZX17716.1"
                     /translation="MKIVSFNINGLRARPHQLAALIDKHQPDVIGLQETKVADEQFPE
                     ADIRQLGYHVHYHGQKGHYGVALLSRQEPLSLHKGFPGDAEDAQRRFIYGTFADAQGN
                     PVTVMNGYFPQGESRDHPTKFPAKQRFYADLQNLLLNQFSPEQALVVMGDINISPEDC
                     DIGIGELNRLRWLKTGKCSFLPEEREWLATLKNWGLVDSFRHLNPAVSDRFSWFDYRS
                     RGFEDEPKRGLRIDVILASQALQARIKDAGVDYDLRGMEKPSDHAPIWLQLGE"
     gene            10981..12363
                     /locus_tag="VFF22_12765"
     CDS             10981..12363
                     /locus_tag="VFF22_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HZX17717.1"
                     /translation="MLRASSAFERDTWIRTVSFLLLGSICYALPWSVFAALPIPSDNS
                     SVLRIQGSNTIGARLGPALVKGLFEEQGLSAIRIEAGAAENEQKIIGKTADGRSVSIE
                     VAAHGSGTGFTSLADGSAELAASSRPIKDSEATSLLSLGDLKSASAEQIIALDGLAII
                     LHPSNPLAALNTAQLAQIFAGEISDWAQLGAPAGSITLYARDDKSGTFDTFKELVLSA
                     NGKTLAANAKRFESSEKLSDSVSQDPNGIGFIGLPYIRHAKAVAIAAGDSQPMLPSLT
                     LIATEDYPLSRRLFLYNQPELSNPWGRALVQFAHSPRGQAIVEQSGFIAQTVQAVKIP
                     ANAQMPANYQTLAQNAQRLSVNFRFQEGSANLDNKALRDLNRLMAYLQEQNKLHDKVV
                     LVGFGDAKADTARAELLSKLRAMAVRRELNKGGVIFRDIIGMGDELPVAANSGDDGRI
                     KNRRVEVWVY"
     gene            12486..13469
                     /locus_tag="VFF22_12770"
     CDS             12486..13469
                     /locus_tag="VFF22_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HZX17718.1"
                     /translation="MDSLASMLTTPLFGTPGWFWLAFLLIILGLLILDLGVLHRDQHE
                     IEMRESLLLYSSYFSVGVAFGCWIWWQLGATKALEFYTGFLVEQSLSMDNVFVMAVIL
                     NFFAIPRRYQHRVLFWGILGVIVLRATMIGLGTALVQNFEWVLYLFGAFLLFTGVRML
                     SAKEEQHPDLSQNRLLQFLRMHIRVTEDMHGGRFLVRQPDRKSGKYLLHATPLLLALV
                     LIELADLVFAVDSVPAVLAISQDPFIVYTSNIFAILGLRALYFALAALMHRFIYLKYA
                     LALVLMFIGGKIFLHDIVKVPALLSLCMTLGLLAGGVVLSLLKTRAPRQEH"
     gene            13474..13683
                     /locus_tag="VFF22_12775"
     CDS             13474..13683
                     /locus_tag="VFF22_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17719.1"
                     /translation="MSSPSIVIEPLVQRGKLRWQVRMGRRSLIFHQEQAARAFAAQLH
                     MRLLWLQQSANPDDEFAPPGTPHSP"
     gene            complement(13732..14961)
                     /locus_tag="VFF22_12780"
     CDS             complement(13732..14961)
                     /locus_tag="VFF22_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZX17720.1"
                     /translation="MDFAYSPKVQQLRERVTAFMETHVYPAEAVFEQQVNEGDRWQPT
                     AIMEELKAKAKAEGLWNLFLPESELGAGLTNTEYAPLAEIMGSSLIGAEPFNCAAPDT
                     GNMEVLVRYANEAQKEQWLKPLLDGTIRSAFAMTEPGVASSDATNMQASAVRQGDEWV
                     INGRKWWTSGACDPRCKIMIFMGLTNPDGPRHQQHSMILVPMDAPGVKVLRPLPVFGY
                     DDAPHGHAEVLFENVRVPYENVLLGEGRGFEIAQGRLGPGRIHHCMRSIGMAERALKL
                     MCERSINRTAFGKPLARLGGNIDHIADSRMEINMARLLTLNAAYMMDTVGNKIAASEI
                     AQIKVVAPNVALKVIDRAIQIHGGAGVSNDFPLAYWYAMQRTLRLADGPDEVHRAAIG
                     KFEIGKYVSKEVMNASR"
     gene            15120..16046
                     /locus_tag="VFF22_12785"
     CDS             15120..16046
                     /locus_tag="VFF22_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX17721.1"
                     /translation="MNLNKVDLNLFVVFDAIYTEANLTRAGQIVGITQPAVSNALARL
                     RETFNDPLFVRTAQGMVPTPMAQNIIGPVRNALQQLRISVQESRIFNPAQANKTFRIS
                     MTDLTEAIVLPPLFQRLRRLAPNVNIESMLARRRETTKELAAGRLDFAVDAPLNTDPQ
                     VRHVKLMEDRYVCAMRRGHPLAKEKISLDEYMGLTHIHISSRRSGLGHVDLALGRMGI
                     QRKIALRSQHYLMATTVLQGTDMAMTVPERFARRNDLHFVELPIKDMTPLETHIYWHE
                     STDQDPANRWMREQIIEISQQATALEKKTYKE"
     gene            16148..16552
                     /locus_tag="VFF22_12790"
     CDS             16148..16552
                     /locus_tag="VFF22_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HZX17722.1"
                     /translation="MSIALQQNDLALDLDSTKAGQVALKFFFNLMEKWDCTVEQQMIL
                     LGSIGRSTLYKYRQLPEVRLPRDTMERVSYLMGIHKSLRILFGDKPSTYEWIKKANSE
                     APFNGNSALQMMLAGGVVDLADVRRYLDGVRG"
     gene            16567..17235
                     /locus_tag="VFF22_12795"
     CDS             16567..17235
                     /locus_tag="VFF22_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003196773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HZX17723.1"
                     /translation="MALPDWRRAYRLVSSSFPPISIFEDVLDPADLEQAYLIEGLTND
                     RLREQAGLLSHVATEDRISGPGSSPLMAAFTHVGAVSRFTAGQYGVYYCADSLQAAVA
                     ETRFHRERFLRATAEPNLELTLRSYVCRIVKPLHDIRANHDHLHDPDLSAYPIAQRFA
                     AELRKQQSWGLLYRSVRHPGGECAALFRPPAASIPQQGVHFRYVWDGKLQQIVNVFEI
                     KEIA"
     gene            17420..17545
                     /locus_tag="VFF22_12800"
     CDS             17420..17545
                     /locus_tag="VFF22_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17724.1"
                     /translation="MKLGPIELSVQEKERVNVPVWAGIGAIIAGGLLFGFAGRKP"
     gene            17593..17925
                     /locus_tag="VFF22_12805"
     CDS             17593..17925
                     /locus_tag="VFF22_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017338395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein tyrosine
                     phosphatase family protein"
                     /protein_id="HZX17725.1"
                     /translation="MPLKLLFICAKNRLRSPTAEQLFADWPGVETASAGVNHDADTPV
                     CPELLAWADLVLVMQRVHRSKLASKFKAHLTNKRVVCLDIPDDYGFMDPALIALLNTK
                     MQRFLPPR"
     gene            18154..18555
                     /locus_tag="VFF22_12810"
     CDS             18154..18555
                     /locus_tag="VFF22_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZX17726.1"
                     /translation="MSATTYSISELARELDITTRAIRFYEEQQMLSPERRGQERIYTA
                     KDKVTLKLILRGKRIGFSLAECKELIELYDPVHGNHKQLHTFMAKIAERRAQLQQQLL
                     DIEQMQLELDTAEERCLAALAETDKKQTVIS"
     gene            18585..19748
                     /locus_tag="VFF22_12815"
     CDS             18585..19748
                     /locus_tag="VFF22_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HZX17727.1"
                     /translation="MSYPSLNFGLGETIDMLRDSVYQFSQAELAPRAAQIDRDNEFPM
                     DMWRKFGDMGLLGMTVEEEYGGTNMGYLAHVVAMEEISRASASVGLSYGAHSNLCLNQ
                     IRKNGTHEQKAKYLPKLCTGEHVGALAMSEPNAGSDVVSMKLRAEKRGDRYVLNGNKM
                     WITNGPDAHTYVIYAKTDISAGSRGMTAFIVERDYKGFSRHQKLDKLGMRGSNTCELV
                     FEDCEVPEENILGAEGGGVRVLMSGLDYERTVLSGGPTGIMTACMDVVLPYVHERQQF
                     KQSIGEFQLVQGKLADMYAGMNASKSYLYNVAKACDRGEESRKDAAAVILYTAEMATK
                     MALDTIQLLGGNGYTNEYPAGRLLRDAKLYEIGAGTSEIRRMLIGRELFNETK"
     gene            19930..21537
                     /locus_tag="VFF22_12820"
     CDS             19930..21537
                     /locus_tag="VFF22_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZX17728.1"
                     /translation="MAILHTHINTRSPEFATNSAAMLTQLNDLRALLARVHEGGGAKA
                     QERHTSRGKLLPRERINRLLDIGSPFLEIGQLAAHEVYGEEVPAAGVVAGIGRVEGVE
                     CMIVANDATVKGGSYYPLTVKKHLRAQTIAQQNRLPCIYLVDSGGANLPRQDEVFPDR
                     EHFGRIFFNQANMSAMGIPQIAVVMGSCTAGGAYVPAMADEAIMVRNQATIFLAGPPL
                     VKAATGEVVSAEDLGGADVHCKTSGVADHYADNDEHALALARRSISNLNWRKLGVLNN
                     RAPINPLYSGEELYGVIPADAKQPFDVREVIARTVDGSVFDEFKALFGATLVCGFAHI
                     HGYPVAILANNGILFAESAQKGAHFIELACQRGIPLLFLQNITGFMVGQKYEAGGIAK
                     HGAKLVNAVACAKVPKFTVIIGGSFGAGNYGMCGRAYDPRFLWMWPNARIGVMGAEQA
                     AGVLVQVKHEQAARAGQSFSAEDEAKLKQPILEQYERQGHPYYSSARLWDDGVIDPAQ
                     TRDVLGLALSASLNAPIEPTTFGVFRM"
     gene            21617..22417
                     /locus_tag="VFF22_12825"
     CDS             21617..22417
                     /locus_tag="VFF22_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-carboxygeranoyl-CoA hydratase"
                     /protein_id="HZX17729.1"
                     /translation="MTDFSTVQLEKDPRGFATLWLNRADKNNAFNAEMIRELILALDA
                     VMADKTLRFLLLRGRGKHFSAGADLAWMQHAATLDYNANLADSRELAELMHNLYQLKI
                     PTLAVVQGAAFGGALGLISCCDMAIGAVDAQLCLSEVRIGLAPAVIIPFVVQAIGERA
                     ARRYALTAERFSGERAQELGLFAECYAADALEQAVADWTSNLLLNSPQAMQASKDLLR
                     EVGSGVLTPALRRYTENAIARIRVSPEGQEGLRAFLDKRKPAWQEPQA"
     gene            22414..24399
                     /locus_tag="VFF22_12830"
     CDS             22414..24399
                     /locus_tag="VFF22_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HZX17730.1"
                     /translation="MSHKPIDTLLVANRGEIACRVMRTAKAMGIKTVAVHSAIDHNAR
                     HVREADLAVNLGGAKPADSYLRIDALIAAAKASGAQAIHPGYGFLSENAGFARAIETA
                     GLIFLGPPASAIDAMGSKSAAKALMDAAGVPLVPGYHGEAQDVETFRVASNAIGYPVL
                     LKAAAGGGGKGMKVVERESELAEALASAQREAQSSFGDSRMLVEKYVLKPRHVEIQVF
                     ADSHGNCLYLNERDCSIQRRHQKVVEEAPAPGLSAELRRAMGEAAVKAAQAIGYVGAG
                     TVEFLLDERGDFFFMEMNTRLQVEHPVTELITGLDLVAWQIRVARGEALPITQEQVPL
                     IGHAIEVRLYAEDPDNDFLPATGTLALYREAAAGPGRRVDSGVSEGDEVSPFYDPMLG
                     KLIAWGENREEARLRLLAMLKETCVGGVRTNLAFLTRVLAHPAFANAELDTGFIPRHE
                     AQLLPPVNELPADFWQLAAEAFVQSEAPLQRHDDPHSPWSNHSGLRLGLPAETDLHLS
                     CNGSERKVRPDNSARLNDEWLICRSELARDAAIASKLAPTEVERRLRAIRQGDTLYLH
                     YAGELHAVTRFDPIAEVEASHQHHGGLTAPMNGSIVRVLVEAGQAVEAGTALVVLEAM
                     KMEHSIRAPHAGTVKELYCNEGEMVSEGTVLVELEEA"
     gene            24498..25400
                     /locus_tag="VFF22_12835"
     CDS             24498..25400
                     /locus_tag="VFF22_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HZX17731.1"
                     /translation="MNFPKQVRLVEVGPRDGLQNEKQPISVADKVRLVDDLSAAGLGY
                     IEVGSFVSPKWVPQMAGSREVFAQIQQLQGITYAALAPNLKGFEGALEAGVREVAVFS
                     AASEAFSQKNINCSISESLERFVPVMDAACQHDIRVRGYVSCVLGCPYDGSVPPEQVA
                     SVARELYAMGCYEVSLGDTIGAGTAGDTRRLIEVVGRDIPRGKLAGHFHDTYGQALAN
                     IYASLLEGINVFDSSVAGLGGCPYAKGATGNVATEDVLYLLEGLGIETGIDMDKLIAA
                     GQRICTVLDKPNGSRVARAKLAQR"
     gene            25548..26888
                     /locus_tag="VFF22_12840"
     CDS             25548..26888
                     /locus_tag="VFF22_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020306635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acid transporter"
                     /protein_id="HZX17732.1"
                     /translation="MLSRITALSVNLVQRYLPSPFVFALLLSFVVLAASMLSTGQSLP
                     QMAQHWGNGFWNLLTFAMQMALILVTGHALARASVLNRLLDRLARIPSTPGQAIVTVT
                     LVALAGSWINWGFGLVIGAVFARALARQVKGVDYPLLVASAYSGFLIWHGGLSGSIPL
                     SLATGGADLERMSGGVLTSAIGVGQTLFTPLNLTIIALLVIGLPLLNWAMHPKQPKTA
                     DPELLVETESKALPRETAAQRLDDSRILGLITVGLALVYFIYYFANSGFVLTLNIVIG
                     LFLFIGLLLHGSPERYTRAVQDSIGGISGIVIQFPFYAGIMGMMVGANADGASLGKQI
                     TDTFVAWSSADTFPVLAFLSAGLVNVFVPSGGGQWAVQGPIMLPAGQALGVAPAVTAM
                     AIAWGDAWTNMIQPFWALPLLGIAGLGARDIMGYCLLILVYSGLVIAGCFYFLT"
     gene            27136..29091
                     /locus_tag="VFF22_12845"
     CDS             27136..29091
                     /locus_tag="VFF22_12845"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HZX17733.1"
                     /translation="MQKPLWTPSKDRIAATRMDAFRRFVNQRHSLQLADYPALHAWSV
                     AQREAFWQAIVEFFEVRFSAQPEAVLQERSAMPSAQWFPGATLNFAEHLLRRRDHHPA
                     LVAIGEDGSREQLSYAELAAHVAGLQRQLQAAGIGMGDRVAAFMPNTWQTLVGMLAAT
                     SLGATWSSCSPDFGTQGVIDRFGQIEPKVLIAAAGYRYAGKNLDLTAKLNEILERLPS
                     LEQLIVAPYSNTQAKAADFNSGAKVALWQDFYQPGGEPEFTQVAFDHPLYIVYSSGTT
                     GVPKCIVHGTGGTLLQHVKELGLHADLQADDTLFYYTTCGWMMWNWLVSGLALGTTLV
                     LFDGSPFHPGAERLIDLIDAENISIFGTSAKFLAALEKAGAKPGSTHKLQRLKAILST
                     GSPLSHESFEYVYREIKADLCLSSISGGTDIVSCFALGNPVLPVWRGELQCKGLGMDV
                     QVWNDAGQAVTGEKGELVCAQHFPSMPVGFWKDDDGSKFQAAYFDTFPGIWAHGDYAE
                     ITTHGGLVIHGRSDAVLNPGGVRIGTAEIYRQVEKVEAVLESIAIGQEWDDDVRVVLF
                     VRLREGVELTDALQAQIRQVIRANTTPRHVPAKIIAVADIPRTISGKIVELAVRNVVH
                     GKSVKNTDALANPQALELYRDLAELRD"
     gene            29162..29890
                     /locus_tag="VFF22_12850"
     CDS             29162..29890
                     /locus_tag="VFF22_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX17734.1"
                     /translation="MVVRWICLAVLLTPLSLSATAQEPLELHMPDAPPLTFVSFQGGY
                     GMVGDVALAAIARAGYLSHIHVTPWARAQQRVSRGHNLLIIPLSRTPEREALYTWIVP
                     ISPLERAFFSLDAPVADFAEARQRYKRIAVGLGTAQMEILKSEGFADEQIVSLKLGDN
                     PARMLELGRVDAWFTGVPEGLYLWPQSDNRLQMSPVLASTDLYLACSKKCDADLQENL
                     RKAVDALRDEGMIQRIQDAYRPVH"
     gene            complement(29919..31139)
                     /locus_tag="VFF22_12855"
     CDS             complement(29919..31139)
                     /locus_tag="VFF22_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HZX17735.1"
                     /translation="MDLNAMLKILSSQDGSDLYLSTGAPPCAKFNGVLKALSADPLKA
                     GEVAAIADSVMDSAQREEFERELEMNLAISIPSVGRFRINIFKQRNEVSIVARNIKME
                     IPKFEDLKLPEVLLKTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
                     PVEYVHKHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFA
                     DTGHLAISTLHANNANQALDRIINFFPEDRRPQLLNDLGNNLKAFVSQRLVKTIDGKR
                     RAAVEVLLGTPTIRDLIKRNEFSEIKEIMEKSKNLGMQTFDQALIDLVNDGSIDEEEA
                     VKNADSANNVRLKLKLYRDNPGTSAPAAAPAPVAAAPTPAAPAASAGEWGLELKLEDI
                     EEEQLPEDPGRKGI"
     gene            31262..31561
                     /locus_tag="VFF22_12860"
     CDS             31262..31561
                     /locus_tag="VFF22_12860"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003300382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZX17736.1"
                     /translation="MNSEEPVMAKAMARHILVKTEAEAAALKKRIAAGEAFDVLARKY
                     STCPSGKKGGDLGEVRPGQMVRAIDQVIFKKPVREVHGPVKSQFGYHLVQVFYRE"
     gene            31580..31942
                     /locus_tag="VFF22_12865"
     CDS             31580..31942
                     /locus_tag="VFF22_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HZX17737.1"
                     /translation="MTIEQIAAFCLSLPGAREDIKWGGVRVFSVAESKMFALFHLDSR
                     DGLAFKVDADLFLGYCDRPGIRPAPYLARARWISMAAPYPLSDGELRELLTRSHQLVV
                     AKLPKRLKLALLLDPSTQ"
     gene            complement(31957..32508)
                     /locus_tag="VFF22_12870"
     CDS             complement(31957..32508)
                     /locus_tag="VFF22_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17738.1"
                     /translation="MLWLKRLFGSVQEQKNTEADRPGQGNFSPAEPQGGQAEVAQEHW
                     SERVARIEAARGGVPGNPTGCVPASIDSTQFSLSVKGDIHSLRIARLNQDEADARRYG
                     IRHKFMHLSALSPSTRPTHASRHGQLFSAEEVRQWWAQDGNAEGCRCALTMILLDEKG
                     NPRDGKLIDKAEKMLAAWNRKKD"
     gene            complement(32615..34570)
                     /locus_tag="VFF22_12875"
     CDS             complement(32615..34570)
                     /locus_tag="VFF22_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HZX17739.1"
                     /translation="MNQDHQQFHASLEAHDDVAINPSANPALSDLLNPARRNVLKGGL
                     TLAALGFFAGGISACRSAGASPLLGFKGVPVQIDPQFDRVLVAEGYRATPFFSWGDPV
                     EAGSPQWNPDASDDWQAQLKQAGDNHDGMDFFAFEGETDRGLLVMNHEYVNPPLHPGN
                     LIDQSKPRPLSEVRKEQAAHGVSVIEVKKAPDGQWQRVMDSPYNRRISALTAMRIAGP
                     LAGHELMQTVADPTGLEVLGTINNCATGITPWGTFLTCEENWHNYFINRDQTDYASRV
                     SHKRYGLAKEGTSKNYAWETADPRFNATPDPQQAHGGYVNEPHRFGWVVEIDPFDPQA
                     QPVKRTAFGRFGRECAALSLGDDGRMAFYSGDDAKGEYVYKFVPSGRFDAANPKANRD
                     LLDEGTLYVARFNADGSGQWLALVYGQNGLTSASGFTSQAEVLLNARAAADLVGATPM
                     DRPEWAAVHSASREVYVALTNNDGRGDKQPTDKANPRPQNLHGQILRFKEQGADPSAE
                     TFTWELFLLAGEQKGAKDANGNALPANLTGTINGDIFSSPDGLAFDNHGRLWIQTDYG
                     DDEAAMQAMGCNQLLCADPSTREVRRFLVGPRGCEITGLAWSPDGKAMWVNVQHPGIS
                     YPASDGQTRPRSTTVLITKNDGGVIGS"
     gene            complement(34647..35438)
                     /locus_tag="VFF22_12880"
     CDS             complement(34647..35438)
                     /locus_tag="VFF22_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZX17740.1"
                     /translation="MDSPTPITWPRLPISRLRNAQAALLTLALHALVLALLLANWPVE
                     KPAAPSISTISMQLVSLPPPAPVVEAAPPVPVDAPPPEVRAPVEPQVDPRIAQQKLEQ
                     AALAHKRVEQKRLEQERQWLAHQRQEQARLEQQEREQAQQRQAAEQLAAQQRQQDADA
                     AAEQARQAEAAAAASRQYLPIAKQAPAYPQRALDKGIEGECTVSYTVNAQGRVEQPEV
                     VGACHPMFIRPSLAAAQSFRYQPRVIDGRAVAVPNVKNTFTYRIE"
     gene            complement(35450..>35633)
                     /locus_tag="VFF22_12885"
     CDS             complement(35450..>35633)
                     /locus_tag="VFF22_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011061987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZX17741.1"
                     /translation="STVGLEEMTAQVTQIIAERGDTQVYIRADQAADYATVVAGIAAL
                     QKGGVSNLGLITEAPQ"
BASE COUNT         6963 a        11187 c        10593 g         6890 t
ORIGIN      
        1 taagcgatca ggccgctcag gaagatcgcc agcatgatca gcgtagtggc gaccgggcgc
       61 aggataaaca ggcgagaggc gttcattggg cgcgcggcgc tcgtttgctt tcaggcgtgg
      121 ccggcgcgct ctgggcggcg accggtgtgt cgccaatcac ctcaaccttg ttgccggagc
      181 gcaaacggtc agtaccttca agcaccaggc gatccccggc cttgagcccc tgctcgacca
      241 cgctgagctg gccgtcactg gcgccgatgc tgatcggcgt cagctgcacc ttgtcctcag
      301 catcaaccac ataggcgaag gtgccgctgg cgccgaactg caacgcggcc gccggcatca
      361 gggtgacgtt ctgcaaggtt ttcacccgca gacgcacgtt gacgaactgg ttgggaaaga
      421 gcatttcttc ggcattggca aaacgcgcct taagcttgac cgtacctgta gcggtgtcga
      481 tcaggttgtc gacgctctcc agctcgcctt cggccagttt gagtttttca ctgcggtccc
      541 aggcctcgac cgcgagggtc gcaccactgc gcagttgctg caccacggcc ggcagttcgg
      601 cttcaggcaa ggtaaacatc acggcaatcg gcagggtctg ggtaattacc accagcggta
      661 actcgtcgcc ggagctgacc aggttgccga catcggcctg acgaatgccc aggcggccgg
      721 taataggtgc gcgaatctga gtgaaatcga ggttcagccg cgctgtggcg acatccgcct
      781 gcaggctctt gaggctgcca cggaactgat tgaccagtgc ttcctgggtg tcgagggtct
      841 gtttggcaat cgaatcttcg gcgtacaggc cgcggtagcg cgccagatcc agctcggcat
      901 tgcgcagctg cgcctggtgc tcctgcagcg cgccctgagc ctgctgcaag gcgatttcat
      961 aagagcgcgg gtcgatcact gcgagcagat cgccggcctt gacctgctgg ccatccttga
     1021 acagcagttt gaccaattcg ccgtctaccc ggccgcgcac attcaccgtg ttataggcgg
     1081 tgacggtgcc cagggccttg agctcaaccg ggaagtcgcc ctgactgaca gtcgccacgc
     1141 gcaccggcac cggcccgcca tcacgccaag ggccaccacg ctgcggcgct tccggagggc
     1201 tgggccagaa ccaccacagc agtacggcga gaacagccag cgcgagtgag ccgagcagcc
     1261 attggcgcga caggttgcga ggacgggtgg tgagctgtgt atcaggcatg gcgactatca
     1321 cttccttggg gagcgtgaac gatatgtagt atcagccggc ctgcaaagcc tctttaccgc
     1381 ctatttacct tttccttacg taactaaatg tctgatgcga atagaaaagt gttcgccata
     1441 aaaatctctg caaacaaaaa cggcctgcaa ggcaggccgt tggacaatcg tttagcgggt
     1501 gttatttgag cacggcaatc gcagcgtcgt agttcggctc atcagcgatt tcgccaacca
     1561 gctcgctgtg cagcaccttg tcgttttcat ccagcaccac tacagcgcgg gcagcaacac
     1621 ccaccagcgg gccgctggca atggccacgc cgtagttctg caggaaatcg gcgccacgca
     1681 tggtcgacag gttgatcacg ttctccagac cttcagcgcc gcagaaacgc gcctgggcga
     1741 acggcaggtc ggtggagatg cacagcacca cggtgttggc cagctggctg gcgctggtgt
     1801 tgaaagtacg cacggaggtg gcgcaggtcg gggtatcaac gcttgggaaa atgttcagga
     1861 ctttgcgctt gccggccagg ctggccaggc ttacatcact caggtcacca gcaaccaggc
     1921 taaacgccgg cgcttgctga ccaatctgcg gcagttgacc ggtgacttca accggggaac
     1981 ctttgagggt gacttgtgcc atgggaacga tccttatcaa cgaaattgga gagggctaac
     2041 aagtaagggc gcgaacccgc caaatggcaa gcccgcgccc tgttgaaggg aggcaggcat
     2101 agctgaccaa ggagtcagac taccctgccc gcccaggcga cacatcaatt ttttgcgaag
     2161 tgatccttgg tcagcttgac cacgatcggg ctgagcaaca acagcgacag caggttagga
     2221 atcgccatca gaccgttgag ggtatcggcc accaaccagg cgaagtccag ctgggcaatc
     2281 gcgccgaatg gcacggctac cacccagaga atgcggaacg gcaggatcga cttggtgccg
     2341 accatgtatt cccagcactt ctcaccgaag tagctccagc cgaggatggt ggtaaaggca
     2401 aacaccacca gggccaacgt caggatataa ccgccgacgc ctggcatcgc ctgttcgaag
     2461 gctgctgccg acagcgctgc accgctggca ccgctggtcc acacgccgga ggtgatgatc
     2521 gccaggccag tcatcgagca gatgatgatg gtgtcgataa aggtcccgag cataccgatc
     2581 atccccgaac gtaccgggct gttggtggtg ccggccgcct gagcgatacc tgcagtaccc
     2641 agacctgctt cgttggagaa aataccgcgc gccacaccga agcgaatcgc tgccatcacc
     2701 gctgcaccgg caaagccgcc agtcgccgca atcggggtaa aggcgtaggt gaagatcatt
     2761 tcaaaggctt tcgggatttc cgaggcgttg accactagca ccaccatagc ggcgatgatg
     2821 taagccacac acataaaggg caccagggtc gccgctactt taccgatacg cttgatgccg
     2881 ccgaggatca ccatgccgac aatcaccatg gtcaccacac cggtcaccca cagcggaatg
     2941 ttgaaggtgg tttgcaaggc tgcagccatg ctgttgacct gcaccatgtt gccgataccg
     3001 aaaccggcaa agccgccgaa gatggcgaac gcggtgccca gccatagcca cttcttgccc
     3061 aggccgtttt tgatcgcgta catcggaccg ccgacgtgct cgccgcgctc atccttctcg
     3121 cggtaatgca ccgccagtac cacttcgcag tacttggtgg ccataccaac caaggcggta
     3181 caccacatcc agaacaatgc acccgggccg ccgaggaaga tcgcggtggc cacaccggcg
     3241 atattgccag taccgacggt ggcggccaga caggtcatca gcgcctggaa cgggctgatc
     3301 tcaccgctct cggcatcgcc tttctggcgg ccttgccaca tcagcttgaa gcctgcgccg
     3361 atcttgctca gtggcatgaa tttcaggcgc aacatgagga ataggccggt gccgagaatc
     3421 agcaccagca tgggcgggcc ccagaccacg ccattgagtt gattgaccag ggtgttgacg
     3481 aattccatgc tcatttcctt attcttgtag tgaacgcgag gtgggtagcc ttcgcttgtc
     3541 cgattgccgg gtaggccagg ctgttacaca gaacgcgagc cggattggcg cgcctgcaaa
     3601 caacggatcg ggctatacca gtcagcacaa cgactgcccg gttatcagcg gcggtcagta
     3661 tatgaagact gtgatagtgg ttttcactgt ttagatttca acgctcagac gttttgagcg
     3721 atgggcgcaa tttaaacgcg aattaactgg gttaatccag cgccggcccg cagaaacgtg
     3781 gtctagaaat cgcgccgata gaacaaatcc agagaactgg ccaagccgct ggcagcctca
     3841 aggtagagcc gtcggctcag ttcatagcgc aaggcgatgg tattcgctgg ctcaaacacc
     3901 cccacgccat agcgcaggct caggcgctcg gacagattgc cgctggccac cacactggtg
     3961 gtcaccccag agccctgggt gtcgagctga aaatcactga tacccaggcc ctgcgccagg
     4021 ctactggcca acggcgcact gccggccatg cccagggcca gcgcggcttg cgcaagcata
     4081 ttattgtcgc caccgccctg ccccagcggc cgccccataa tcaggtagga caacgcctgc
     4141 tcctggctca tcgccggttc ggaaaacact tcgctggtcg gttgcgcagc attgccgctc
     4201 agccgtaaac cggcggtgac atcatcgacc tggcgcacgg cctcgatatc gagaaacggc
     4261 tgatcaatcg gcccggcgaa cagcaagcgc gcacggcgca cagtcaggcg ctgaccgtat
     4321 gcgcggaagc ggcccttgtt caggcttagc tcgccacggg tgtccaggtc attgccgata
     4381 tgcacacgac caagcagttc agcgtccagg ccaaagccgc taaaggtcag gcgctgctgg
     4441 ccaacctcga cttcaatatc catggcaatc gccgtagcct gtcgttgcgt gctctcgtgg
     4501 cccaccacct gggcatcgct ggaaacctgc acggttgaag gcggcagctc gcgcacttca
     4561 atcttgccgc tggggatcag cactttaccg gccaccgata gctgctcatc acgtagactc
     4621 agacgcagat ccggttcgac ttccagcgcg gcgtagggct ctaccgtcac cggcagccgg
     4681 ctgccccgca gctgcatatc cgcgagcaat ccatcggtcc agctcagctc gccattcagg
     4741 ctgccctgcc cctgctcgcc actgcgccaa tcaccctcca gctgcagccg ctcaccctcg
     4801 atgcgcgcct gcagttgcag ctgctcaata ttcattggca gctcgccacc gcttatttcg
     4861 ccattaacta cacgcaggtt gccattgatc tgcggcgcca gtagcccgcc tgacaaggtg
     4921 ccagaaccgt tgagctgccc agcaatacgc tcgaccatcg gcacaaacgg acgagccagg
     4981 gccaggtcaa gaccattcaa gcggaaactg ccggacagcg gtttgctcgc cgtgcgtgga
     5041 tcgagctgag cctgcagcga cagctcacca atcgccggac cgcgcaactc cagcaggctg
     5101 tcgatgcgtt gcgggcgcaa ttgcgcgctc aggcgcaggc tgtcgtaggc aaaatccagc
     5161 cactcctgct gatcacgaat acgccagatg ccgatgccgg catccagctc caggctgcca
     5221 ttcggtccgc tggccggcag atccagctgc agtcgaccat tcaactcgcc ttgccaggcg
     5281 aaatccttgg gccacagggc cgccaagctt tccatgggga agttgctcag ctgatagttc
     5341 agcttgggct gcggcagcag gcgctgctcc gtgccacaca ggctggcacc gcccgagcgc
     5401 caacaatggg ctcccagact gagctgccca ttggccagcc gcaccagcgt ggctggctgt
     5461 tgcaggcgcc aatcctggcc accgctttgc acttcgccac ggctcaggct gccgcgccag
     5521 ttgcccttgt ccagcttgcc ggccagggcc aactggcttt gcagcaaagg gccttgcagc
     5581 tgcagttcaa ggctttgttg ctgctggttg ccagcagcgt tggcggtcag atggcccagg
     5641 tcggtttcac cgagctgaat gccctgcgca tccagagtga ttcggccacg ctgggcactg
     5701 ttcaggctgg cgtcgagctt gagctgttgc aggcgctggt cgtcaaaggc caaccgctgg
     5761 ccctgcagct gcagctggcc ttggggcgct tgcagacttc cggccaggtc cagctgaccg
     5821 ttaagttggc cctgcaagcc aggccagagc tggcccagtc gcggcatcgc cagcagcaac
     5881 tggccgttca gctgttgccg caaggcggcc ttgccactga tgcggttatc acccagacgc
     5941 acatctagag cactcagctc gccctgctcc agaccgccgg caaacttcgc cagcaacttg
     6001 gccggctgac cacgcaaacg gccggtgagg tcgatatcgg cgttaaggtt gagctgctga
     6061 ttgatcaggc tgcccgagct gtttagtggg ccagctaggc tgccgggcag ttcggccagc
     6121 cagtaagccg ggtcaagctg actcaactgc aaacgcacat cccagctgaa ctgctcggca
     6181 aaccccaggc tgacctggcc ttcggcgcgg ccttggccgg cttgcaggcg aagttcgggc
     6241 agatacagat tctgcaggtc gccgcttagc gggctttgta ggctaaaagc accggccggg
     6301 ccatccagct cggcggcgaa atggccgagg tagttgccat cctgataggc caactcgcca
     6361 tcaagcatgc gcaacgcaac aggcgctggc tcgtccagag gatagaggcg ctgccagggg
     6421 aagttctgcc agttgaagcg gctgtccagg ctcaggccct gctgccagct caactcaccg
     6481 ctgacgttta cttgctgctc tggttcggcc gtgaggctca ggctgctgat ggtcgcgccg
     6541 tcagccttga gctgccccgc caatgcaagg gcaattggcc cttcagttgc tggcaggctg
     6601 gcactgccgg tgacttggta gccatcctgc agatcgccct gggcatttag ctcaacctgc
     6661 tcaaggtttg acgtatcggg cagttcagcg ctggccttga aggcggcaac ctgcaaacgc
     6721 agttgcgccg gcagatgctc cgccagcggt tgcagccagc ccgtcagctc accttgcaga
     6781 tagccgctgc tgctggccgt gacctgcacc cgctcgcgca actcaccctc aagctgcaat
     6841 gccacaggcc aggcctgatg ctcgggcgcc ggcaattgca gttgcccact cagttgcagc
     6901 ggccagttgc cggaggtttg caccttgccc tgcagcgcca cctgcagatc tgcgctacgc
     6961 aggctgaggt tgtcgatctg aatgccttgc accgtccagc gtgcggccag ctgcaggcct
     7021 tgcagctgct cgacgccatt gagttgcagg ctgtcgatcc gcacctgccc cagttgcaat
     7081 gccagcggca ggctcaggtc cggcaagctg aagggctcgc tgctgggttg cccatcttct
     7141 ggaaagacca agctgatcgg cccgctgtgc agctgctcga tacacagggt caggcgcaac
     7201 aagcaggctg gcgaccaatc cagactaggc gccatcacct gcacgctatt ctcgccctgc
     7261 tgccagcgca gctgtgtcgc gctccagccg ctgcccaggc ggccattgaa gtcgtccacc
     7321 tgcaaaccgg gcacctgtgc caataaccag cgactgccgc cctgggtgcc taatagcaga
     7381 ctcaggctga tggccagcac aagcagcagc cctagcagac tgaacagccc atagcgcagt
     7441 ccgcgcttca cagttccggc cccatggaaa agtgcaggcg tacgccgcct tgatcatcca
     7501 gcggatgcgc caggtccagg cgcagcggcc ccagtggcga cacccagcgc acgccaaggc
     7561 ccaccgcgct tttcagcgat ggaatctcca gcgaatcgaa ggtattgccc tgatcgaaga
     7621 agctcgccag acgccagcgc tcggtcaggc tgtactggta ctccaagctg ctggtaaaca
     7681 gataacgccc gccgattttg tcgccggcac ggttctccgg cgacaggctc tggtagtcat
     7741 agccgcgtac gctctggtcg ccaccggcaa agaaacgcag cgacggcggc accttggcaa
     7801 aaccgtcgga ttcagtgccg cccacttgca cccggccgag cagccggtgc cgggtaaata
     7861 gcgtggtcag gcctttaagc tgaacattgc cgtgcagcac attggcatcg gacaacagcc
     7921 ccttggccgc gccggacaga tcaaactgca ggcggtagcc gtggttgggg tcaatctggt
     7981 tgtcactctg caggtaggaa taactcaggc ccggcatcag caacgtactg gtgccggcgt
     8041 catcacccag gtcgtactca tcgtgctgcc atttcagcaa cagcacccgc tgccagccgc
     8101 tgtcgagttt gctatgccac tccgggccaa aggtcagcag acggctgctg ctgtcggtat
     8161 tggccaggtc ttcagcctga tagccgccgg cgatgcgcag cttatcggtc agcggcggat
     8221 caagcggcac gtcgtaccac agaccgacat tctgccgcgg cgccgacagc tcgaactccg
     8281 cgccatagct gtgcccctgt gggttggccc agtgccgcgt ccagttggcc cggccacgcg
     8341 ggccaacgtc ggtggaaaac cccaagccaa ggcccatgct gcgcggttta cgcgcctgca
     8401 attgaacctg caccggaatc tgctcggcgt tcgcactggc tgggttggca tccacctgca
     8461 ccgactcgaa ataaccactg gaacgcagcg cctgatacag ctcggcaatc agtgcggagt
     8521 cataaggcgt atccgccggg aacggcacca tgcgctgcag caattgttca tcgaacggcg
     8581 catcgccgac aaagctcact gcgcccagtt gatagcgcgg tccgctgaca taaaccagct
     8641 cgatatcggc aaccccagcc tcggggtcaa tcgtcaggcg ctgactgcta aaacgcccgg
     8701 caaagaagcc ataacgcgag gcctgattct gaatatcgcg cttggccgcg tcgtaacgcc
     8761 cgtgattgag ctgcgccccg ctgcgcagct gctggtcatt gggtgtacgg aaatcgcgca
     8821 gctgcgctgc cgggccgtcg atacgtacca gcacattacg caggcgtacc ggctcacccg
     8881 gctggatgcg taaggtcagg cgcggtgttg cgctctcgct tacctcggtc gtgatctgcg
     8941 cctggtagta gcccagggct tgtgcggctt tttccgcctg gctctcggcc acccggcgaa
     9001 agcgttgcag cgcttcggca tcacgatcgc ccaggctgcc gatataaccc tcgatattgc
     9061 ttttcagcgc gctatcagcc ggctcaaccc gcacgcgcag ctgtgcttcg gccagggccg
     9121 tagcactcag caatagaaga aacagcgcgg tgaaaaggtg gggagaaaca ggcatgaggg
     9181 cgatgctagc acgagctcag ggccgactta gagcaccagt gcgggcaaat agttgcgact
     9241 cgatgtgtcg gcagacacac cgagcaggcc gtttttcagg cactggcctt gattgccact
     9301 tgcgggcttg gatggaaaaa cacgtgttcg ataatcgggc cgatggccac ctcaccgatt
     9361 tcctcgtaac cctgtcgctt ataaaaatcc aggtaatgtg agttgccggt atccagcacc
     9421 acaccctggg agctggcatc ttccgcgcac cagttgtgca gcgcctcaag cagttgctca
     9481 ccgaggtgct tgccctggta ttccgggtga atgcccagca gcggcaacac gtggtatggc
     9541 cccggcggca ggcaggcgat caccgcatcg tggtattcca gatagcgctt ggtgcaacgg
     9601 aaacctgtgg tcagcagcat gcgcatgcgc cagccccagc tttcagtgat gtccatgcgc
     9661 cgctgcggag gtgcgatcag cgccatgccg atcaggcggt catcgatcag caagccaatc
     9721 gccggcagat cctcactaaa atgctgctgt accagctcac gcaccgtggc gcgcactcgc
     9781 tggtcataac ctggccgctc ggcctcgaac agataggcaa aggtcggatc atggcgataa
     9841 gcgtggtaga gcaacgagcg tgcttcgcgg gcgtaaccgc catcgagcat gcgaacttcg
     9901 gcgagactgt tgggcattga cttgacctct gttgttcttg atttactggg cctgcagcca
     9961 cgttagcact gcttacccag cgccgccaca ggctggcagg taggaccagt aatgggctag
    10021 catcgcgggc tttccagagc cacagccagg actgccgccc atgaagatcg tctccttcaa
    10081 tatcaatggc ctgcgtgcac gcccgcatca actggccgca ttgatcgaca agcaccagcc
    10141 ggatgttatc ggcctgcagg aaaccaaggt cgccgacgag cagttccccg aggcagatat
    10201 tcgccagctt ggttatcacg tgcattacca cggccagaaa ggacattacg gcgttgccct
    10261 gctctcgcgc caggagccgc tgagcctgca caaaggcttt cccggtgatg ccgaggacgc
    10321 ccagcggcgc tttatctacg gcacctttgc cgatgctcag ggcaaccctg tcacggtaat
    10381 gaatggctac ttcccacaag gcgaaagccg cgaccacccg accaagttcc cggccaagca
    10441 gcgtttctat gccgacttgc agaacctgct gctcaaccag ttcagccccg agcaggcact
    10501 ggtggtgatg ggcgatatca atatttcgcc ggaagattgc gacatcggca tcggcgaact
    10561 caatcgcctg cgctggctga aaaccggcaa atgcagcttc ctgccggaag aacgcgagtg
    10621 gctggccacc ctgaaaaact ggggcctggt cgacagcttt cgccatctca atccggcggt
    10681 gagtgatcgc ttcagctggt tcgattaccg cagccgcggc ttcgaggacg aacccaagcg
    10741 tggcctgcgc atcgatgtga ttctcgcctc ccaggcgttg caggcgcgga tcaaggatgc
    10801 cggcgtcgac tacgatctgc gcggcatgga aaagccatcg gaccatgcgc cgatctggct
    10861 gcagctgggc gagtaaccct gtactagagc gttcaattgg aaaagtgact gtcatattgc
    10921 agtcactttt ttgtcttatc gtcgcggcac tttcactcac ccatataagg tgtttgccgc
    10981 atgttgcgcg cctcctctgc cttcgaacgt gatacctgga ttcgcaccgt cagctttctg
    11041 ctgctcggct ccatctgcta tgccctgccc tggtcggtat tcgccgccct acccatcccc
    11101 agcgataaca gcagcgtgct gcgcatccaa ggctccaaca ccattggcgc gcggcttggc
    11161 ccggcgctgg tcaaaggcct attcgaagaa cagggcctga gcgccattcg catcgaagcg
    11221 ggcgcggcgg aaaacgagca gaagatcatc ggtaagaccg ccgatggccg cagtgtaagc
    11281 attgaagtgg cggcccacgg ctcgggcacc ggctttacct cattggccga tggcagcgcc
    11341 gaattggccg cctcgtcccg gccgatcaag gacagcgaag ccaccagcct gctaagcctg
    11401 ggcgacctga aaagcgccag cgccgaacag atcatcgccc tcgacggcct ggccatcatc
    11461 ctgcatccaa gcaatccgct ggcggcgctg aacaccgcgc agctggcgca gatatttgcc
    11521 ggcgagatca gcgactgggc gcagctcggt gcgccagccg gttccattac cctgtacgcc
    11581 cgtgatgaca agtccggcac cttcgacacc ttcaaggagc tggtacttag cgccaacggc
    11641 aaaaccctgg ccgccaacgc caaacgcttc gagtccagcg aaaagctctc cgactcggtc
    11701 agccaggacc ctaacggcat cggctttatc ggcctgcctt atatccgcca tgccaaagct
    11761 gtggcgattg ccgccggcga ctcgcaaccc atgctgccga gcctgaccct gattgctacc
    11821 gaggattacc cgctgtcgcg ccggctgttc ctctataacc agccggaact gagcaacccc
    11881 tggggccgtg cattggtgca gttcgcccac agcccccgtg gccaggcgat tgtcgagcag
    11941 agcgggttta tcgcgcagac cgtgcaggcg gtaaaaatcc ccgccaacgc gcaaatgccg
    12001 gcgaattatc agaccctggc gcagaacgct cagcgcttgt cggtgaactt ccgctttcag
    12061 gaaggtagcg ccaacctcga caacaaggcc ctgcgcgacc tcaaccgcct gatggcctac
    12121 ctgcaggagc agaacaagct gcacgacaag gtggtgctgg tgggcttcgg cgatgccaag
    12181 gccgacaccg cccgcgccga gctgctgtcc aagctgcgcg ccatggccgt acgtcgcgag
    12241 ctgaacaagg gtggcgtgat cttccgcgac atcattggta tgggtgatga actgccggta
    12301 gcggccaaca gcggcgatga cgggcggatc aagaaccgcc gggtggaagt ctgggtctac
    12361 tgagcggctt acgcctgcta atctttttca agcgtggcgc ctggcccctc tggcggtgag
    12421 ccttcaccgt gatgtcggag tcactgagtt gattctcaac ttaggcagac attcgaggtg
    12481 gctccatgga tagccttgca agcatgctca caaccccact gttcggcacc cctggctggt
    12541 tttggctggc ctttctgctg atcatcctcg gtttgctgat actcgacctc ggcgtgctgc
    12601 accgcgatca gcatgaaatc gagatgcgcg aaagcctgct gctgtacagc agctacttca
    12661 gcgttggtgt ggcctttggc tgctggatct ggtggcagct gggcgccacc aaggcgctgg
    12721 agttctacac cggctttctg gtcgagcaat cgctgtcgat ggacaacgtg ttcgttatgg
    12781 cggtgattct caacttcttc gccatcccgc ggcgctacca gcaccgcgtg ctgttctggg
    12841 gcattctcgg ggtgatcgtg ctgcgcgcaa cgatgatcgg cctgggcacg gcgctggtgc
    12901 agaacttcga atgggtgctg tacctgtttg gcgcctttct gctgtttacc ggcgtacgca
    12961 tgctttcggc caaggaagaa cagcaccccg acctcagcca gaaccgcctg ctgcagttcc
    13021 tgcgcatgca tattcgagtg accgaggata tgcacggcgg gcgcttcctg gtgcgtcagc
    13081 cggatagaaa atcgggcaag tacctactgc atgccacgcc gttgctgctg gcgctggtac
    13141 tgatcgaact ggctgacctg gtgtttgccg tggacagcgt gccagcggtg ctggccatat
    13201 cccaggaccc gtttatcgtc tacacctcga acatcttcgc gattctcggc ctgcgcgcgc
    13261 tgtactttgc cctggcggcg ctgatgcacc gttttatcta cctcaagtac gccctggcgc
    13321 tggtgctgat gtttattggc ggcaagatct ttctccacga tatcgtcaag gtgcccgccc
    13381 tgctctcgct gtgcatgacc ctcggcttac tggcaggcgg agtggtactg tcgctactga
    13441 aaacccgcgc acccaggcag gagcattgat cgtttgagtt caccgagcat tgttatcgag
    13501 ccgctggtgc agcgcggcaa gctgcgttgg caagtgcgca tgggccgccg cagcctaatc
    13561 ttccatcagg aacaggctgc ccgcgccttc gcggcgcagt tgcatatgcg cctgctctgg
    13621 ctgcaacaga gcgctaatcc cgatgatgag tttgctccgc cgggaacacc gcattcgcca
    13681 taacgcacaa ggcccgcaaa tgcgggcctt gtggtttttc agtgacgcgc tttagcggct
    13741 ggcgttcatc acttctttcg acacgtactt gccgatttcg aacttgccga tggccgcgcg
    13801 gtgcacttca tccgggccgt cggccaggcg cagggtgcgc tgcatggcgt accagtaggc
    13861 cagcgggaag tcgttggaca ccccggcgcc gccgtggatc tggattgccc ggtcgatcac
    13921 tttaagcgcg acgttgggtg caaccacctt gatctgggcg atttcgctgg cggcaatctt
    13981 gttgcccacg gtgtccatca tataagccgc gttcagcgtc agcaggcggg ccatattgat
    14041 ctccatacgc gagtcggcga tgtgatcgat attgccgccc aggcgggcca gcggcttgcc
    14101 gaaggcagta cggttgatcg agcgctcgca catcagtttc agcgcgcgct cggccatgcc
    14161 gatggagcgc atgcagtggt gaatacggcc tgggccaagg cgaccctggg caatctcgaa
    14221 accacggccc tcacccagca gcacgttctc gtacggtacg cggacgtttt cgaacagtac
    14281 ttcggcatgg ccgtgtggcg cgtcgtcgta accgaatacc ggcagcggac gcagcacttt
    14341 cacgcctggg gcgtccatcg gcaccagaat catcgagtgt tgttgatggc gcgggccatc
    14401 cgggttggtc aagcccataa agatcatgat cttgcagcgt ggatcgcaag cgccggaagt
    14461 ccaccacttg cggccgttga tcacccactc atcgccctgg cgtacggcgc tggcctgcat
    14521 gttggtggca tccgaggacg ccacgcctgg ctcggtcatg gcgaaggcgg aacggatggt
    14581 gccgtccagc agcggcttga gccactgttc tttctgcgct tcgttggcgt agcgcaccag
    14641 cacttccata ttgccggtat ccggcgcggc gcagttgaac ggctcggcac cgatcaacga
    14701 gctgcccatg atttccgcca gcggcgcgta ttcggtgttg gtcaggccgg cgcccagctc
    14761 ggactccggc agaaacaggt tccacaagcc ttcagcttta gccttggctt tcaactcttc
    14821 catgatcgcg gtgggctgcc agcggtcgcc ttcattgacc tgctgctcga acacggcttc
    14881 ggctggatat acatgggtct ccataaacgc ggtgacgcgc tcacgcagct gctgaacctt
    14941 gggggagtag gcgaaatcca tgatggctac cttctgacgc tgatttttgg ggagttcggt
    15001 gaagtggaaa tgatgctaga acagggctta ggatttatct aatctattct caccgtgtat
    15061 aaacattcat caccgatata tgattcactg ataccgacaa cctgcggagc gagcgtgtaa
    15121 tgaatctgaa caaggtcgac ctcaacctct tcgtcgtctt cgatgccatc tacaccgagg
    15181 ccaatctgac ccgtgccggg cagatcgtcg gcattaccca gcccgcagtg tccaacgctc
    15241 tggcgcgttt gcgcgaaacc ttcaatgacc cgctgtttgt gcgcaccgcc cagggcatgg
    15301 tgcccacgcc aatggcgcag aacatcatcg gcccggtgcg caatgcgctg cagcagctgc
    15361 gcatctccgt gcaggaaagc cgcatcttca acccggcgca ggccaacaag accttccgta
    15421 tcagcatgac cgacctcacc gaggccatcg tcctgccgcc gctgttccag cgcctgcgcc
    15481 gcctagcacc caatgtaaat atcgaaagca tgctggcccg gcgccgtgaa accaccaagg
    15541 agctggccgc cggccgtctg gacttcgccg tcgacgcgcc gctcaacact gacccgcagg
    15601 tgcgccacgt caagttgatg gaagaccgct acgtgtgcgc catgcgccgc ggccatccgc
    15661 tggccaagga gaagatcagc ctggatgaat acatgggcct gacccatatc catatctcca
    15721 gccgccgcag cggcctcggc catgtcgacc tggccctcgg ccgtatgggc atccagcgca
    15781 agattgccct gcgctcgcag cattacctga tggccaccac cgtactgcaa ggcaccgata
    15841 tggccatgac cgtgccggaa cgcttcgcca ggcgtaacga cctgcacttt gtcgagctgc
    15901 caatcaagga catgacgccg ctggagaccc acatctactg gcacgaaagc accgaccagg
    15961 atccagccaa ccgctggatg cgcgagcaga ttatcgagat ttcccaacag gccacggccc
    16021 tagagaaaaa aacctacaag gaataagcat ttaaaaggtg tgggcctgat cgcaacgact
    16081 gcaactcatt gctgaatttc tctatgtgga ctaatattta tattaattcc acatggggat
    16141 aaatattatg tccatcgccc tgcagcaaaa tgacttagcc cttgatctcg attcgaccaa
    16201 ggccggtcag gttgcgctga agtttttctt caatttaatg gagaaatggg actgcacggt
    16261 ggaacagcag atgatcctgc tgggctcaat cggccgtagc acgctgtaca agtaccgcca
    16321 acttcccgag gtgcgcctgc cccgtgacac tatggagcgc gtgtcctatc tgatgggtat
    16381 tcacaaatcc ctgcggattc tgttcggcga caaaccctcg acctatgaat ggatcaaaaa
    16441 agccaatagc gaagcaccgt tcaatggcaa cagtgcgctg cagatgatgc tcgcgggtgg
    16501 tgtcgtcgac ctggccgatg tgcgccgcta cctcgacggt gtaagaggtt gagcgcgccg
    16561 ccagccttgg cccttccaga ttggcgacgg gcctatcgcc tggtctccag ctcgttcccg
    16621 ccgatttcca tattcgagga tgtactcgat cccgccgatc tggaacaggc ctacctgatc
    16681 gaaggcctga ccaatgatcg cctgcgtgaa caagccggcc tgttgtctca cgtggcgacc
    16741 gaggatcgga tcagtggccc aggttcatcg ccgctgatgg ccgccttcac ccatgtcggc
    16801 gcagtcagcc gtttcaccgc aggtcagtac ggcgtctact actgcgccga cagcttacag
    16861 gctgcggtgg cggaaacccg ttttcaccgt gaacgcttcc tgcgcgccac tgccgagccc
    16921 aatctagagc tgaccctgcg cagctatgtg tgccgtatcg tcaagccgct gcacgatatt
    16981 cgcgcgaacc atgaccatct gcatgacccg gacttgagcg cctacccgat tgctcagcgt
    17041 ttcgctgccg aactgcgcaa acagcagtcc tgggggttgc tgtatcgcag cgtgcgtcat
    17101 cccggcggcg aatgcgccgc actgtttcgt ccgcctgcgg cgagcatccc gcagcagggc
    17161 gtgcattttc gttatgtgtg ggatggcaag ctgcaacaaa tcgtcaatgt gttcgagatc
    17221 aaggaaatcg cctagcaagc gtcagcactg tgcttcaatg gcctggacgg ccctgccttt
    17281 gaaaccgctg gcgattagct caggcgccgc atgaggaata cagcatgaat tcaatccgga
    17341 ttatctccat cgcactggtc gtggcaggcc tgctggggct gctgtatggc ggtttcagct
    17401 acaccaagga cacaccgcag tgaagctcgg cccaatcgaa ctctcggtgc aggagaagga
    17461 aagagtcaac gtgccggttt gggcaggcat cggcgccatc atcgcgggcg gtttgctgtt
    17521 cggctttgcc ggcagaaagc cttaagtcat ttatccatag cgcttagctc ggcgccgatc
    17581 agggacgatt gtttgccact taaactgcta tttatctgcg ccaaaaatcg cctacgcagc
    17641 cctaccgccg aacagctatt cgctgattgg cccggtgtgg aaaccgcctc ggccggcgtc
    17701 aatcatgacg ccgacacgcc ggtctgcccc gagctgctgg cctgggccga tctggttctg
    17761 gtgatgcagc gagtgcatcg cagcaaactg gccagcaaat tcaaagctca cctgaccaac
    17821 aaacgcgtgg tttgtctgga tatcccggac gactacgggt ttatggaccc cgctctgata
    17881 gcactgctga acaccaagat gcagcgcttt ttgccgccac gttaaatccc ccgatctatc
    17941 tcaccgctta gccagcgagg cgcgcatccg tccgctcgcg tcttcaaggc agtagccatt
    18001 cattattgct tttttaacta tccgtcctaa gccattgttt taacggctac tgggcgaacc
    18061 tagaaaacac atcaactagg cgcccgctca tggctgttga ctaaacatcg ccgcagcctt
    18121 acgttaacgt aaaggtaaat aagagcactg cgtatgtccg ccaccaccta ctccatttcc
    18181 gagctggccc gcgagctgga tatcaccacc cgcgccattc gtttctatga agaacagcag
    18241 atgctcagcc ccgaacgccg aggccaggag cgcatctaca ccgccaagga caaggtcacc
    18301 ctcaagctga tcctgcgcgg aaagcgcatc ggcttctccc tggccgagtg caaggaactg
    18361 atcgagctgt acgacccggt gcatggcaat cacaagcagc tgcacacctt tatggcgaag
    18421 atcgccgagc gccgcgccca gttgcagcag caacttttag acatcgaaca gatgcagttg
    18481 gaactggata ccgccgaaga gcgttgcctg gcagccttgg ctgaaactga taaaaaacaa
    18541 accgttatct cctgaactta attcgaacaa ttacaggtgg aaccatgagc tatccgtccc
    18601 tcaacttcgg tctcggcgag acgatcgata tgctgcgcga ctcggtgtat cagttctcac
    18661 aagccgaact ggcaccacgt gccgcacaga tcgaccgcga caacgagttc cccatggaca
    18721 tgtggcgcaa gttcggcgac atgggcctgc tcggcatgac cgtggaagag gaatacggcg
    18781 gcaccaacat gggttacctg gcccatgtgg tggccatgga agagatcagc cgtgcctcgg
    18841 cctcggtcgg cctgtcctac ggcgcacact ccaacctgtg cctcaatcag atccgcaaga
    18901 acggcaccca cgagcagaag gccaaatacc tgcccaagct gtgcaccggc gaacacgtcg
    18961 gcgctctggc catgagcgag ccgaacgccg gctccgacgt ggtgtcgatg aagctgcgcg
    19021 ccgagaagcg cggcgaccgc tatgtactca acggcaacaa gatgtggatc accaacggcc
    19081 ccgatgccca cacctatgtg atctacgcca agaccgacat cagcgccggc tcacgcggca
    19141 tgaccgcgtt tatcgtcgag cgcgactaca agggcttctc ccgtcaccag aagctggaca
    19201 agctgggcat gcgcggctcc aacacctgcg agctggtgtt cgaggattgc gaagtaccgg
    19261 aagagaacat cctcggcgcg gaaggcggtg gtgtgcgcgt actgatgagc gggctggatt
    19321 acgagcgcac cgtgctctcc ggcggcccga ccgggatcat gactgcctgc atggacgtgg
    19381 tgctgcccta cgtgcacgaa cgccagcagt ttaagcagtc gatcggcgag ttccagctgg
    19441 tacagggcaa gctggccgac atgtatgccg gcatgaatgc ctccaagtcc tacctgtaca
    19501 acgtggctaa ggcctgcgac cgcggcgagg aatcgcgcaa ggacgccgcc gcagtaatcc
    19561 tctacaccgc agaaatggcc accaaaatgg ccctggacac catccagctg ctcggcggta
    19621 acggctacac caacgaatac cccgccggcc gcctgctgcg cgacgccaag ctttacgaaa
    19681 tcggcgccgg caccagcgaa atccgccgca tgctgatcgg ccgcgaactg ttcaacgaaa
    19741 ccaaatagcc tgacggagcg cgccagatcc tgatggttcc cacgctccag cgtgggaacc
    19801 tgaaaccgga cgttccgcgt ccgctttggg gacgcagagc gtccctggcg gcactcccac
    19861 gcagagcgtg ggagcgatca gaacacagag tccccggatt gcatccgggc tacgaatgga
    19921 gtgagccgaa tggccatcct gcatacccac atcaataccc gttcgccgga gttcgccacc
    19981 aatagtgcgg caatgcttac tcagctcaac gacctgcgcg ccctgctcgc ccgcgttcat
    20041 gaaggcggcg gggccaaggc ccaggaacgc cacacctcgc gcggcaagtt gctgccgcgc
    20101 gaacggatca accgcctgct ggatatcggc tcgccgttcc tcgaaatcgg ccaactggcc
    20161 gcccatgagg tatacggcga agaggtgccg gcggctggtg tggtggccgg tatcggccgg
    20221 gtcgagggtg tggaatgcat gatcgtggcc aacgacgcca cggtaaaagg cggcagctac
    20281 tacccgctga ccgtgaaaaa gcacctgcgc gcgcagacca tcgcccaaca gaaccgcctg
    20341 ccgtgcatct acctggtgga ttccggcggt gccaatctgc cgcgccagga tgaggtgttc
    20401 ccggaccgcg agcacttcgg gcggatcttc ttcaaccagg ccaatatgtc ggccatgggt
    20461 attccgcaga tcgccgtggt catgggctcc tgcaccgccg gtggcgccta cgtgccggcc
    20521 atggcggatg aagcgatcat ggtgcgcaac caggccacca tcttcctcgc cggcccgcca
    20581 ctggtaaaag ccgccaccgg tgaggtggtc agcgccgaag accttggcgg tgccgatgtg
    20641 cactgcaaaa ccagtggtgt ggccgaccat tacgccgaca acgatgaaca cgccctggct
    20701 ttggcccggc gcagcatcag caatctcaac tggcgcaagc tcggcgtgct gaataaccgt
    20761 gcgccgatca atccgctata cagcggcgaa gagctgtacg gggtgattcc ggcggatgcc
    20821 aaacagccgt tcgatgtgcg tgaagtgatc gcccgcaccg tcgatggcag cgtgtttgat
    20881 gaattcaagg cgctgtttgg cgcaaccctg gtgtgcggct ttgcgcatat tcacggctat
    20941 ccggtggcga tcctcgccaa taacggcatc ctgtttgcgg aatccgcaca gaaaggcgcg
    21001 cactttatcg aactggcctg ccagcgcggc attccgctgc tgttcctgca gaacatcacc
    21061 ggctttatgg tcgggcagaa gtacgaagcc ggcggcatcg ccaaacacgg ggccaagctg
    21121 gtcaacgccg tggcctgcgc caaggtgccg aaattcaccg tgatcatcgg cggcagcttc
    21181 ggcgccggta actacggcat gtgcggccgc gcctacgatc cacgcttttt gtggatgtgg
    21241 ccgaatgcgc gcattggcgt gatgggcgcc gaacaggccg ccggcgtact ggtgcaggtc
    21301 aaacacgaac aggccgcccg cgccgggcag agcttctccg ccgaggatga agccaagctc
    21361 aagcaaccga tcctcgaaca gtacgagcgc cagggccacc cttactactc cagcgcccgc
    21421 ctgtgggacg acggggttat cgacccggcg caaacccgcg acgtattggg cctggcactg
    21481 tccgccagcc ttaacgcgcc gatcgagcca accacctttg gcgtgttcag aatgtaatcc
    21541 gcgagtaccg tagggtgcgc cgtgcgcacc gctcttcaac gccatggtgc gcgcagcgca
    21601 ccctacgaag atgaccatga ccgacttcag caccgtacaa ctggaaaaag acccacgcgg
    21661 ctttgccact ctctggctca atcgcgcaga caaaaacaac gcctttaacg ccgaaatgat
    21721 ccgcgagctg attctcgccc tcgacgcggt gatggccgac aaaaccctgc gtttcctgct
    21781 gctgcgcggc cgtggcaagc atttcagcgc cggcgcggac ctggcctgga tgcagcacgc
    21841 cgcgaccctg gactacaacg ccaacctggc cgactcgcgc gagctggccg agctgatgca
    21901 caacctgtat cagctgaaga tccccaccct ggccgtggtg caaggcgcgg cctttggcgg
    21961 tgccctgggg ctgatcagct gctgcgatat ggccatcggt gccgttgacg cccagctgtg
    22021 cctgtccgaa gtgcgcatcg gcctggcgcc ggcggtgatc atcccctttg tcgtgcaggc
    22081 cataggcgaa cgcgctgcgc ggcgttatgc cctgacggcc gagcgcttta gcggtgagcg
    22141 ggcgcaggag ttggggctgt ttgccgaatg ctatgccgcc gacgctctag agcaggccgt
    22201 ggccgactgg accagcaatc tgctgctcaa tagcccgcag gccatgcagg ccagcaagga
    22261 tctgctgcgc gaagtcggca gcggcgtgct gaccccggcg ctgcgccgtt ataccgagaa
    22321 cgcgattgcc cgtatccgcg taagccccga aggtcaggaa ggtctgcggg cattcctgga
    22381 caaacgcaaa cccgcatggc aggagccgca agcatgagcc ataagcctat cgacaccctg
    22441 ctggtggcca accgtggcga gatcgcctgc cgggtgatgc gcacggccaa ggccatgggc
    22501 atcaagaccg tggcagtgca cagcgccatc gaccataacg cccgtcatgt acgcgaagcc
    22561 gatctggcgg tgaatctggg tggtgccaag ccggcggaca gctacctgcg catcgacgcg
    22621 ctgatcgccg cagccaaggc cagcggtgcc caggccatcc accctggtta tggttttctg
    22681 tcggaaaacg ccggttttgc ccgtgccatc gaaaccgcag gtttgatctt tctcggcccg
    22741 cccgcctcgg ctatcgacgc catgggcagc aaatccgccg ccaaggcgct aatggacgct
    22801 gccggcgtgc cgctggtgcc gggttatcac ggtgaggcgc aggatgtgga aaccttccgc
    22861 gtagcctcga acgcaatcgg ctacccggtg ctgctcaagg ccgccgctgg cggcggtggc
    22921 aagggcatga aggtggtcga gcgcgaaagc gaactggccg aggccctggc cagcgcccag
    22981 cgcgaggcgc agtcctcgtt cggcgactca cgcatgctgg tggaaaaata cgtacttaaa
    23041 ccgcgtcatg tggaaatcca ggtctttgcc gactcgcatg gcaactgcct gtacctgaat
    23101 gagcgcgact gctcgatcca gcgtcgccac cagaaggtcg tcgaagaagc accggctccg
    23161 ggtctgtcag ccgaactgcg tcgcgccatg ggcgaggccg cggtcaaggc cgctcaggcc
    23221 atcggctatg tcggcgccgg cacagtggaa tttctgctgg atgagcgcgg cgacttcttc
    23281 tttatggaga tgaacacccg cctgcaagtt gaacatccgg tgaccgagct gatcaccggc
    23341 ctcgatctgg tcgcttggca gatccgcgtc gcccgtggcg aggcgctgcc gatcacccag
    23401 gagcaggtgc cgctgattgg ccacgccatc gaggtgcggc tgtatgcgga agatccggac
    23461 aacgacttcc tgcctgccac cggcaccctg gcgctgtatc gcgaagccgc cgccggccca
    23521 ggccgtcggg tagatagcgg ggtcagcgaa ggcgatgaag tctcgccttt ctatgacccg
    23581 atgctcggca agctgatcgc ctggggcgaa aaccgcgaag aggcgcgcct gcgcctgctg
    23641 gccatgctta aggagacctg cgtcggtgga gttagaacca atctggcctt tctcacccgc
    23701 gtgctagccc acccggcctt tgccaatgcg gagctggata ccggctttat tccgcgtcat
    23761 gaagcacagt tgctgcctcc cgtgaacgag ttgccggccg atttctggca attggctgcc
    23821 gaggcctttg tgcaaagcga agcgccgctg cagcgccacg acgatccgca ttccccttgg
    23881 agcaaccata gcggtctgcg cctgggttta ccggcggaaa ccgatctgca cctaagctgc
    23941 aatggcagcg agcgcaaagt acgccccgac aatagcgcac gcctgaacga cgaatggttg
    24001 atctgtagga gcgagcttgc tcgcgatgcc gcgatcgcga gcaagctcgc tcctacagag
    24061 gtagagcgtc gcctgcgtgc cattcgccag ggtgacactc tgtatctgca ctatgccggt
    24121 gagctgcacg cggtcacccg cttcgacccg attgccgaag tcgaagccag ccatcagcat
    24181 cacggcggcc tcaccgcccc catgaacggc agtatcgtgc gcgtgctggt cgaggccggt
    24241 caggcggtcg aagccggcac tgcgctggtg gtgctggaag cgatgaagat ggaacacagc
    24301 atccgtgcgc cccatgccgg caccgtcaag gagctgtatt gcaacgaagg cgagatggtc
    24361 agcgagggca ccgtgctggt ggagctggaa gaagcataag catggtaatc ggtgcgcaca
    24421 gcgcacccta cgttgcacgc agcgccacaa atcgtagggt gcgccgtgcg caccattggc
    24481 cccaaaggag acgccaaatg aattttccca aacaggtccg cctggtcgaa gtcggcccgc
    24541 gcgacggttt gcagaatgag aaacagccga tcagcgtggc cgacaaggtg cgcctggtcg
    24601 atgacctgtc tgccgcaggc ttgggctata tcgaggtggg cagctttgtc tcgcccaagt
    24661 gggtgccgca gatggccgga agcagagagg tttttgcgca aatccagcaa ctgcaaggca
    24721 ttacctacgc ggcgctggca cccaatttga aaggctttga aggcgcgctg gaagcgggcg
    24781 tgcgcgaagt agcggtattt tccgccgcat cagaagcctt ctcgcagaag aatatcaact
    24841 gctcgatcag cgaaagcctt gagcgctttg tcccggtgat ggacgccgcc tgccagcatg
    24901 acatccgcgt gcgcggttat gtctcctgcg tgctgggctg cccgtatgac ggcagcgtgc
    24961 cgcccgagca ggtggccagc gtggcccgtg agctgtacgc catgggctgc tatgaggttt
    25021 cccttggcga caccataggt gctggcaccg ctggcgacac gcgccgatta atcgaagtgg
    25081 tgggccgcga tattcccaga ggcaaattgg ccgggcactt ccatgacacc tacggccagg
    25141 ctttggccaa tatctacgcc agtctgctcg aaggcatcaa cgtattcgac agctcggtcg
    25201 cgggtctggg cggctgccct tacgccaaag gcgccactgg caatgttgcg accgaagatg
    25261 tgctgtacct gcttgagggc ctgggcatcg aaaccggtat tgatatggac aagctgatcg
    25321 ccgccggcca gcgcatttgc acggtgctgg ataagcccaa tggctcgcgg gtggcgcggg
    25381 caaaactggc tcagcgctag ccgccaggcg acaagggtta ggcaggtgac tcgaggaaca
    25441 tcgctggcgc gcagcgttcc taaggtggat ggccaagcca atcagccccg accacctgat
    25501 cgaccaggct cagggacttc acaagaacaa caacagagga tgtcgacatg ctaagcagaa
    25561 tcaccgcgct cagcgtcaat ctggtgcagc gctacctccc ctcgcccttc gtattcgccc
    25621 tgctgctcag ctttgtcgtg ctagccgcaa gcatgctcag caccgggcaa agcctgccgc
    25681 agatggccca gcactggggc aacggtttct ggaacctgct caccttcgcc atgcaaatgg
    25741 cgctgatcct ggttacgggc catgccctgg cccgcgcatc ggtactcaac cgcctgctcg
    25801 accgcctggc acgcattccc agcacgcctg gccaggcgat tgtcacggtg acgctggtgg
    25861 ccctggctgg ctcctggatc aactggggct tcggcctggt tatcggtgcc gtgttcgccc
    25921 gcgctctggc ccgccaggtc aagggtgtcg actatcccct gctggttgcc tcggcttatt
    25981 cgggcttttt gatctggcat ggcgggcttt ccggttccat ccctctatca ctggccactg
    26041 gcggcgccga tcttgaacgc atgagtggcg gcgtgctgac ctcggctatc ggcgtcgggc
    26101 agaccctgtt tacccccctg aacctgacca tcattgccct gctggtgatc ggcctgccat
    26161 tgctcaactg ggcgatgcac cccaaacagc caaaaaccgc cgacccggaa ttgctggttg
    26221 aaaccgagag caaagccttg ccgcgcgaga cggccgcaca acgcctggat gacagccgca
    26281 tattgggcct gattactgtc ggcctggccc tggtttattt catctactac ttcgccaaca
    26341 gcggcttcgt cctcaccctg aacatcgtca tcggtctgtt tctgtttatc ggcctgttac
    26401 tgcatggctc accggaacgc tacacccgcg ctgtgcagga cagcattggc ggcatcagcg
    26461 gtatcgttat ccagtttccc ttctatgccg ggatcatggg catgatggtg ggcgccaatg
    26521 ccgacggcgc ttcgctcggc aaacagatca ccgacacctt cgttgcctgg tcttcagcgg
    26581 acacctttcc ggtgctggcc tttctcagtg cgggtctggt caacgtcttt gtaccctccg
    26641 gcggaggcca gtgggcagtg cagggaccga tcatgctgcc agccggccag gcactgggcg
    26701 tggcaccagc ggtgaccgcc atggcgattg cctggggcga tgcctggacc aatatgatcc
    26761 agccgttctg ggccctgccg ttgctcggca tcgccggtct cggcgcgcgc gacatcatgg
    26821 gttactgcct gctgatactg gtgtattccg ggttggtgat tgcaggttgt ttctactttc
    26881 tgacctgacc ctcccaccac aggtgtcaag aaaccctgac acctgtaaag cctgtcgtgc
    26941 gcgcaggcgc ggggctacaa gggcaatgga atgccagcgc cacgcgcgcg cgcaagtcgc
    27001 tgaattagga gtaagtgtcg cctggagtca aggcgccccg cggcccttag gctagcgtgc
    27061 cacagaccct gcagcacgct gcaaccgata agcaatattc gctgccgcga ggccggcagc
    27121 cacagaggaa acgacatgca aaagcccttg tggacgccgt ccaaggaccg tatcgccgcg
    27181 acccgaatgg acgccttccg ccgcttcgtc aaccagcgcc acagcctgca gctggccgat
    27241 tacccggccc tgcatgcctg gagcgtggcg caacgcgagg ccttctggca ggcgattgtc
    27301 gagttcttcg aagtgcgttt tagcgcgcag cctgaagccg ttttgcagga acgcagcgcc
    27361 atgcccagcg cccagtggtt tcccggcgcg acgctgaact ttgccgaaca cctgctgcgc
    27421 cgccgcgatc accaccctgc cctggttgcc atcggcgaag atggcagccg cgagcaactg
    27481 agctatgccg aactggccgc ccatgtcgct ggcctgcaac gccaactgca agccgcaggt
    27541 atcggcatgg gtgaccgcgt ggctgctttt atgcccaaca cctggcaaac cctggtcggc
    27601 atgctggctg ccacgagcct gggagcgacc tggtcaagtt gctcgccgga cttcggcacc
    27661 cagggcgtga tcgatcgctt cggccagatc gaacccaagg tgctgatcgc cgccgccggc
    27721 taccgctacg ccggcaagaa tctcgacctg accgccaagc tcaatgaaat cctcgaacgc
    27781 ctgccctcgc ttgaacagct gattgtggcg ccctactcca atacccaggc caaagccgcc
    27841 gatttcaact cgggagccaa ggtcgcactg tggcaggact tctaccagcc cggcggcgaa
    27901 cctgagttca cccaggtggc cttcgaccat ccgctgtata tcgtctattc cagcggcacc
    27961 accggtgtgc ccaaatgcat cgtgcacggc actggcggca ccctgttgca gcacgtcaag
    28021 gaactcggcc tgcacgccga tctgcaggcc gacgacacgc tgttctacta caccacctgc
    28081 ggctggatga tgtggaactg gctggtctca ggcctggccc tgggcaccac cctggtgctg
    28141 ttcgacggct cacccttcca cccaggcgcc gagcgcctga tcgacctgat tgatgcggaa
    28201 aacatcagca tcttcggcac cagcgccaaa tttctcgccg ctctggaaaa agccggcgcc
    28261 aagccgggca gcacgcacaa actgcagcgc ctcaaggcca ttctgtcgac cggctcgccg
    28321 ctgtcccacg aaagctttga gtacgtttac cgcgagatca aagccgatct ctgcctgtcg
    28381 tcgatctccg gcggcaccga tatcgtctcc tgcttcgccc tcggcaaccc agtgttgccg
    28441 gtgtggcgcg gtgagctgca gtgcaagggc ttgggcatgg acgtgcaggt gtggaacgac
    28501 gccggccaag cggttacagg cgaaaaaggc gaattggtct gcgcgcagca tttcccgtcg
    28561 atgccggtgg gtttctggaa ggatgacgac ggcagcaagt ttcaggcggc ctacttcgac
    28621 accttccccg gcatctgggc tcacggcgat tacgcagaaa tcaccaccca cggcggcctg
    28681 gtgattcacg gccgcagtga cgcggtactt aaccccggcg gcgtgcgtat cggcactgcc
    28741 gaaatctatc gccaagtgga aaaggtcgaa gccgtgctgg agtccatcgc catcggccag
    28801 gagtgggacg acgatgtgcg ggtggtgcta tttgtacgct tgcgcgaggg agtcgaactg
    28861 accgacgcgc tacaagcgca gatccgccag gtgatccgcg ccaacaccac accgcgccat
    28921 gtgccggcga aaatcatcgc ggtggcggat atcccgcgca ccatcagcgg caagatcgtc
    28981 gaattagcgg tgcgcaacgt ggtgcacggc aaatcggtga aaaacaccga cgccctggcc
    29041 aatccgcagg cgctggagct gtatcgcgat ctcgcagagc tacgggactg acgtaccaga
    29101 aaaccagcct gcccagcatg cagtgtctat actcccagcc agtcgctgct ggagtgttga
    29161 tgtggttgtg cgctggatct gtctcgctgt attgctgacc ccactgagcc taagcgcaac
    29221 agcacaggag ccgctggagc tgcatatgcc ggacgcgccg ccgctgacct ttgtcagctt
    29281 tcagggcggc tacggcatgg tcggcgatgt ggcattggcc gccattgccc gtgcggggta
    29341 tctcagccat atccatgtaa ccccctgggc tcgcgcccag cagcgggtca gccgcggcca
    29401 caacctgctg atcatcccgc tttcgcgcac tccggagcgg gaagcgctgt acacctggat
    29461 cgtccccatt tcgccacttg agcgcgcctt cttcagcctc gacgcgccag tcgccgactt
    29521 tgccgaggcg cgccagcgtt ataagcgtat cgccgtcggc ttgggcaccg cacagatgga
    29581 aatactcaag agtgaggggt ttgccgatga gcagattgtc agcctcaagc ttggcgataa
    29641 ccccgcacgc atgcttgaac tgggccgtgt cgatgcctgg tttaccgggg tgccggaagg
    29701 gttgtacctg tggccgcaaa gcgacaacag attgcagatg agtcccgtgc tggccagtac
    29761 cgatctgtac ctggcctgct ccaaaaagtg cgatgcagac ttgcaggaga atctgcgcaa
    29821 ggcggtcgac gcgctacgtg atgaaggcat gatccagcgt attcaggacg cctaccggcc
    29881 cgtgcactaa cggcggacat ccctgtcgcc agcggtgctc aaatgccttt gcggccgggg
    29941 tcctcaggca gctgctcctc ttcgatatcc tccagtttca gctccagccc ccattcaccc
    30001 gccgacgccg caggcgcagc aggagtcggt gcggcggcca ctggcgcggg cgcagcggca
    30061 ggcgctgagg tacctggatt gtcgcggtag agcttgagtt tgaggcgcac gttattcgct
    30121 gagtcggcgt ttttcaccgc ttcttcttca tcaatcgagc catcgttgac caggtcaatc
    30181 agcgcctgat cgaaggtttg cataccgagg tttttcgact tttccatgat ctccttgatc
    30241 tcggaaaact cgttgcgctt gatcaaatcg cgaatggtcg gcgtgcccag cagcacttcc
    30301 accgccgccc ggcgcttgcc gtcgatggtc ttgaccagac gctgggagac aaacgccttg
    30361 aggttgttgc ccaggtcatt gagcagttgc gggcggcggt cttccgggaa gaaattgatg
    30421 atgcggtcca gcgcctgatt ggcgttgttg gcgtgcagcg tggagattgc caagtggccg
    30481 gtgtcggcaa aggccagggc atgctccatg gtctcgcggt cgcggatctc gccgatcagg
    30541 attacgtccg gcgcctggcg cagggtgttc tttagcgcgg catggaagct gcgggtatcc
    30601 acccccactt cccgctgatt gatgatggat ttcttgtgct tgtgcacgta ctccactggg
    30661 tcttcgatgg tgataatgtg gccgccgctg ttgcggttgc ggtagtcgat tagcgcagcc
    30721 agagaggttg atttacccga accagtgccg ccgacaaaca gcaccaggcc gcgcttctcc
    30781 atcaccgtct tgagcagcac ctccggcagt ttgaggtctt cgaacttggg gatttccatc
    30841 ttgatattgc gagcaacgat cgacacctcg ttgcgctgct tgaagatatt gatgcgaaaa
    30901 cgtccaacgc tgggaatgga aatcgccagg ttcatctcca gctcacgctc gaactcctcg
    30961 cgctgcgcgc tatccatcac cgagtctgca atggccgcca cctcgccggc cttgagcggg
    31021 tcggcgctga gcgccttgag cacgccattg aacttggcgc agggcggcgc accggtagaa
    31081 agataaaggt cagacccgtc ctggctggac aggattttca gcatggcatt gaggtccata
    31141 acgagatttc ccgcgagttg gatgtattac gttaggccaa gattgcgcat cgcttctggg
    31201 aaaatcagag cgtgcccggt tcaagcgcta agcggcaaag aatactggca caatacgccg
    31261 attgaattcc gaggagcctg tcatggctaa ggcaatggcc cgccatattc tggtgaaaac
    31321 cgaagcagaa gccgctgcat tgaaaaaacg cattgccgct ggcgaagcct ttgacgtgct
    31381 ggcgcgcaaa tactcgacct gcccctccgg taagaaaggt ggcgacctgg gtgaagtacg
    31441 gcctgggcaa atggtgcgcg ctatcgatca agtgattttc aagaagccgg tacgtgaagt
    31501 ccatgggccg gtgaaaagcc agtttggcta tcatctggtg caggttttct accgcgagta
    31561 acacaggagc atcccctgca tgaccatcga gcaaatcgcc gcgttctgcc tgagcctgcc
    31621 gggtgcccgt gaagacataa aatggggcgg cgtgcgggtg ttctcggtgg cagagagcaa
    31681 gatgttcgcc ctgttccacc tagacagccg cgacggtctc gcttttaaag tcgatgccga
    31741 cctgtttctc ggttactgcg accgccccgg catccgccct gccccttatc tagctcgtgc
    31801 gcgctggatc agcatggccg cgccctaccc gctaagtgat ggtgaactgc gcgagctgct
    31861 aacgcgctcc catcagctgg tggtagccaa actgcccaaa cgtttgaaac tggctctgtt
    31921 gctcgacccg agcacgcagt aacaagaagc tcaaacctag tcttttttcc gattccaggc
    31981 agcgagcatc ttttctgcct tatcgattaa ttttccgtct ctcggattac ccttttcatc
    32041 caataaaatc attgtgagtg cgcagcggca accctccgca tttccatctt gtgcccacca
    32101 ctgacgcacc tcctccgcac taaataattg cccatgcctg cttgcatgag tcggcctagt
    32161 tgacggtgag agcgcagaga ggtgcataaa cttgtgcctg attccatagc gcctcgcgtc
    32221 agcctcatcc tggtttaggc gggcgatccg cagcgaatga atatcgccct taaccgatag
    32281 tgaaaactgc gtgctatcaa tgctggcagg tacgcagcca gtcgggttac cagggacacc
    32341 acctctagca gcctctatgc gtgccaccct ttcactccaa tgctcttgag ctacctctgc
    32401 ttgaccacct tgcggctctg caggcgagaa gtttccctga ccaggcctat cagcctcggt
    32461 atttttttgt tcctgcactg acccgaaaag tcgtttcaac caaagcatgc cgactgctcc
    32521 tatcggtatt aaaaccccat ctgttttcag tagcccaata aaacctacga accaggcatg
    32581 taacacacct agcatgtatt ttggcagaac tggcttagct gccaatcacc ccgccatcat
    32641 ttttggtaat tagcaccgtg gtcgaacgcg gtcgggtttg gccgtcgctg gccgggtagc
    32701 tgataccggg atgctgcaca ttcacccaca tcgccttgcc atccgggctc caggccaggc
    32761 cggtgatttc gcagccgcgc gggccgacca ggaagcgcct gacctcgcgg gtgctcgggt
    32821 ccgcgcagag cagctggttg caacccatgg cctgcatcgc cgcttcatcg tcgccgtagt
    32881 cggtctgaat ccacaggcgg ccgtggttgt cgaaggccag gccatcgggt gaggaaaata
    32941 tatcgccatt gatggtgccg gtgaggttgg ccggtagcgc attgccgttg gcatccttgg
    33001 cgcctttctg ctcgccagcg agcaggaaca gctcccaggt gaaggtttca gcgctaggat
    33061 cggcgccctg ctccttgaaa cgcaggattt gcccatgcag gttctgcggg cgcgggttgg
    33121 ctttgtcggt gggttgtttg tcgccgcgac catcgttatt ggtcagggcc acatacacct
    33181 cacgactggc agaatgcacc gccgcccact cggggcgatc catcggcgtt gcgccaacca
    33241 agtcagcggc ggcgcgggcg ttaagcagca cttcggcctg gctggtaaac ccactggcgc
    33301 tggtcaaccc gttttgccca tacaccaggg ctagccattg accgctgcca tcggcattga
    33361 aacgcgcgac atagagagtg ccttcatcga gcaggtcgcg gttggccttg gggttggccg
    33421 catcgaagcg cccgctgggg acgaacttgt agacgtactc gcccttggca tcgtcgccgg
    33481 agtagaacgc catgcggccg tcatcaccca ggctcagggc cgcacattcg cggccaaaac
    33541 gaccaaaggc ggtgcgtttg accggctgcg cctgtgggtc gaaggggtcg atttccacca
    33601 cccagccaaa gcgatgcggc tcgttgacat agccaccgtg ggcttgctgc ggatcagggg
    33661 tggcgttgaa acgcgggtct gcggtctccc aggcgtagtt tttactggtg ccttctttgg
    33721 ccagaccata gcgcttgtgc gacacccggc tggcgtagtc ggtctgatca cggttgataa
    33781 agtagttgtg ccagttttcc tcgcatgtga ggaaggtgcc ccagggcgtg ataccggtgg
    33841 cgcagttgtt gatcgtgccg agcacttcaa ggcccgtcgg gtcggcaacg gtttgcatca
    33901 gctcatgccc ggccagcggg ccggcgatgc gcatggcggt cagggcgctg atgcggcggt
    33961 tgtagggcga atccataacc cgttgccact ggccgtctgg ggcctttttc acttcgatga
    34021 ccgacacacc atgggcggcc tgttccttgc gcacctcgct aagcggccgt ggcttgctct
    34081 ggtcgatcag gttgccgggg tgcagcggcg ggttgacgta ctcatggttc atcaccagca
    34141 ggccacggtc tgtttcgcct tcaaaggcga aaaaatccat gccgtcgtgg ttgtcgccgg
    34201 cttgtttcaa ctgcgcctgc caatcgtcgc tggcatccgg attccactgc ggcgagccgg
    34261 cctcgaccgg gtcgccccag gagaaaaacg gcgtggcacg gtagccttca gcgaccagca
    34321 cgcggtcgaa ttgcgggtcg atctgcaccg gcacgccctt gaagcccagc agcgggctgg
    34381 ccccggcact gcggcaggca ctgatgccac cggcgaagaa gccgagcgcg gccagggtga
    34441 ggccgccctt gagcacattg cgccgcgcag gatttagcaa atcacttagc gccgggttgg
    34501 cgctgggatt aatcgccaca tcatcgtggg cttcaaggct ggcgtgaaac tgttggtgat
    34561 cctggttcat cacggctctc gcaaaaagtt ggcgcgcacc ggcccacacc gctcgataca
    34621 gagcagcctg gacggcgggc atgggtttat tcgatacggt aggtaaaggt gtttttcaca
    34681 ttgggcacgg cgacggcgcg gccatcgatc acccgcggct ggtagcgaaa gctctgcgct
    34741 gcggctaatg agggacggat aaacatgggg tggcaggcgc caaccacctc aggctgttcg
    34801 acccggccct gggcgttgac cgtgtagctg acggtgcact cgccctcgat gcccttgtcc
    34861 agcgcccgct gtgggtaagc cggtgcctgc ttggcaatcg gcaggtactg acggctggcc
    34921 gccgcagcgg cttcggcctg gcgcgcctgc tcggcagctg catcggcatc ctgctgacgt
    34981 tgctgcgctg ccagctgttc ggcggcttgg cgttgttgcg cttgctcgcg ttcttgctgc
    35041 tcaaggcgcg cctgctcctg acgctgatgc gctaaccact ggcgttcctg ctcaagcctc
    35101 ttctgctcca cgcgcttgtg cgccagtgcc gcctgctcca gtttttgctg ggcgatacgc
    35161 ggatcgacct gcggctccac cggcgcacgc acttccggcg gcggggcgtc gaccggcaca
    35221 ggaggcgcgg cttccaccac tggcgccggt ggcggcaggc tgaccaattg catgctgatg
    35281 gtactgatgc tcggcgcagc gggcttttcc accggccagt tggccagcaa caacgccagc
    35341 accaacgcat gcagggccag ggtcagtagc gccgcctggg cattgcgcag acgcgagatt
    35401 ggcaatctgg gccaggtaat tggagtggga ctgtccatca gcgcgctgct cactgcgggg
    35461 cctcggtgat caggccgagg ttactcaccc cgcccttctg caaggcggcg ataccggcga
    35521 ccacggtggc gtagtcggca gcctggtcgg cgcggatata cacctgggta tcgccgcgct
    35581 cggcgatgat ctgcgtcacc tgagcggtca tttcctccag gcccacggtg ctg
//
LOCUS       DASRUM010000196        10264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1770577, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000196 DASRUM010000000
VERSION     DASRUM010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 10264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10264
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770577"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..470)
                     /locus_tag="VFF22_12890"
     CDS             complement(126..470)
                     /locus_tag="VFF22_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HZX17742.1"
                     /translation="MNLYKRLASLLLLLSLSLPAVALNLNEAMSALGDAKASGQLGEM
                     PNGYLGVVSNGGQAAEIAQLINQARRAEYQKLAAQNGIQLSDVEAIAGQKAIDKTPAG
                     QYIQLEGKWQRK"
     gene            complement(483..674)
                     /locus_tag="VFF22_12895"
     CDS             complement(483..674)
                     /locus_tag="VFF22_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HZX17743.1"
                     /translation="MRLRSCFAVLLLGLLSTACTPTVQLAMPNEPININLNVKIEHEI
                     YIKVDKALDSMFSESSGLF"
     gene            complement(690..3278)
                     /locus_tag="VFF22_12900"
     CDS             complement(690..3278)
                     /locus_tag="VFF22_12900"
                     /inference="COORDINATES: protein motif:HMM:NF023167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HZX17744.1"
                     /translation="MPSRRTWLRLISCLVLLLVLLGAYAYLSLSRLLEREQIENLEWR
                     GLGLSTQGIQLDQLSLQHPSGAVQLQQVQLRWSGFSLALPFWQQVQITGLQLNLPSQP
                     EPASDDSTGFALPLEQLAAIISLLPQRVQIDALQIELPCADTRCQLLGDLQLLKQAAQ
                     LDALLNLQHQQDQLSWHAQLQGDATTAALQLSLAINQQPQLQLSSSLQQNAVGQLWQG
                     DFTGDLQQSVVLQSWLSQWLPNAAGTLPEAPAAAQLKASWQLQLAPGALNVAQLQQAS
                     GQLQANANLPEPWPIPATGQLQGSFNLSAKALDGQWLADSLNADLNLKQIANDLISGV
                     PAELYPETLQLTIQATQTPDNLAPQLVDRALPLHLQLSGQGRSQFELNGTLILANGLP
                     WGLQLLDSSLSASTPAFQLNDWKIGALQTQLQLDAYLDAQQLDLALGKGAELSLQHLR
                     STDMQAQQLKASSSGLKLQAQLAAGTLQDWQLEGPLDLSAQLQHGQLQPQRWYWQGPV
                     TASQLQAELDGTLRNDAGLQLKLQAQYSSAKGLSLQAQLAELFLRSGNPLKGSFTAWP
                     ALLELNNGRLNADASLTLASDQQLPTVKLELSGKGLGGIYDRTALEGLDGRITVRVDP
                     KQLQLELTELRLAQANPGIPLGPLQLSGNYSAALQTPTQGQLQLRQAEAALMGGKLQL
                     APGQWSLAAEPLLFPVQAQGLELEQLFILYPTEGLAGTGTLDGNLPLQISSQGVTIEQ
                     GQLSARAPGGQLQFHSERIRALGRSNPAMQLVTQSLEDFRFTTLSSQVNYDQQGKLAL
                     AMRLEGQNPAIEQGRPIHFNINLEEDIPTLLASLQLTDKVSDIIKQRVQQRMLQRNAK
                     TAPTEP"
     gene            3427..3795
                     /locus_tag="VFF22_12905"
     CDS             3427..3795
                     /locus_tag="VFF22_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003157715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HZX17745.1"
                     /translation="MSALIRPVADADAVLAKALLNTREQLGLTQQELADIIGVHRTAI
                     SRWAEGGLRVHSKTGELALLLIRVYRSLFALFGGNLADMQHFLRTDNRHLDAPPLSLM
                     MQVQGLVRVVEYLDAIRGKG"
     gene            3852..4604
                     /locus_tag="VFF22_12910"
     CDS             3852..4604
                     /locus_tag="VFF22_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HZX17746.1"
                     /translation="MDIWQHCQGPAQIKPLRGRLVRLVESQAQVATLQLVDTLAEQAL
                     LEELLETSKPPLPQSAEPLHYLLKTPFRYPPLRWGSRFGSVHEPSLFYGALRIDTAMA
                     EAAYYRFLLWDGMSIAPPSGRILSEHCTFEARYQVERGIQLQHPPFADFQAELCHASA
                     YQPCQAVGAAMRGAGVEAFEYRSARCPLKGNNVALFVPGALAEKRPRNLLSWLCETTD
                     DYVAFKNAQVPDTPRVFRLDAYQVDGRLPRPA"
     gene            4986..5390
                     /locus_tag="VFF22_12915"
     CDS             4986..5390
                     /locus_tag="VFF22_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012053440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HZX17747.1"
                     /translation="MPRAITRLPIAATTAAPLPTRVALWLLDSPRLGHAPSVKRFAGH
                     LLKQPALQGVVQAQSRLGQMLCRDCGNPRDRRMGFELLRQAARAGDMGAQLELGQLYS
                     QPRNNEPLQARHWLELAAGQGSLEARQLLKQL"
     gene            5835..7196
                     /gene="rmuC"
                     /locus_tag="VFF22_12920"
     CDS             5835..7196
                     /gene="rmuC"
                     /locus_tag="VFF22_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HZX17748.1"
                     /translation="MLQERLNNAQLAQTGLAAQLDDCREEIAEISEIKADQQAELAAL
                     RREADLLQQERQIAREAAQTWASQREQQEAEIRRLDAQRAALSAELKEQQDSHQQRLS
                     DLQGSRDELRAQFAELAGKIFDEREQRFAETSQQQLGQLLDPLKERIQSFEKRVEESY
                     QQEARERFSLGKELERLQQLNQRLGDEATNLTRALKGQKTQGNWGELVLERVLEHAGL
                     EKGREYQTQVSLKSADGERFQPDVLIQLPGDKQVVVDAKVSLTAYQQYIAAEDDASRQ
                     LSLKQHVLSLRSHLKGLSLKDYQRLEGLHSLDFVLLFVPIEAAFAAALQADPGLFQEA
                     FDQHIVIVSPTTLLATLRVIDSLWRQERQSQNAREIAERAGALYDKFVAFIQDLDEMG
                     ARLQQLDKAYAGARNKLCDGRGNIISRVENLKLLGARASKSLPAELLEQAAGVMVLDE
                     ASE"
     gene            complement(7309..8250)
                     /locus_tag="VFF22_12925"
     CDS             complement(7309..8250)
                     /locus_tag="VFF22_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZX17749.1"
                     /translation="MNAPAVLPYKELQAEQLWVKGDGVQLAAYRWGKANGPTLLLVHG
                     YPDNHEIWLPLVRELAGDYQIIAYDVRGFGASDIPRKRLDYHLEKLANDLEAVIKAIS
                     PEQPVHLVAHDWGSIQAWEAVTEPRIQPLLASYTSISGPCLDHVGHWMRDRLKQKTPR
                     ALLQVLGQLLSSWYIALFHTPLLPELTWRLGLDRAWPQLLHKVEGLRNVPISSSQRSD
                     GVHGVKLYRANFIRSLFQPRLRHTQVPVQLIVPTRDRFVRPQLFDQLQHWAPTLQRRE
                     ANAGHWQLLAEPQQLAAWLREFTAHTQAAAETGPISA"
     gene            complement(8609..9865)
                     /locus_tag="VFF22_12930"
     CDS             complement(8609..9865)
                     /locus_tag="VFF22_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HZX17750.1"
                     /translation="MPEALDTLLNFASALAAGLLIGAERGWQGRNTEDTQLVAGIRTF
                     ALSSLLGAFAMLLGEHFGVVAWAVIFSGFALLVVASYFGELQRLGDLGLTSEVALLIT
                     FLLGSLAMAGYAGLAAAGAVAVALLLSLKQSLHGALQRLSEAEVSGALKLLFISLVLL
                     PALPNQGYGPWQAFNPYAIWWMVVLIASIGFAAYVAIRLVGTRHGLLITALLGGVVSS
                     TAMTVTLARLAQGRNLQAILACGLLATSALMFPRVLLEVGLVNASLLPQLAWPLGIAT
                     LVYAGGALLYFRRAGTESEEQAEPPLKNPFELGPALRFAALLALILLMVEAAQHWLGD
                     AGVYLVALLAGLADVDAITLSLARSAKGELGAQVAVQGIFLAALSNSLVKGFLVALIG
                     GRKLALMTLPVMAAGLLAGAAVLLLL"
     gene            10014..>10264
                     /locus_tag="VFF22_12935"
     CDS             10014..>10264
                     /locus_tag="VFF22_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020293454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HZX17751.1"
                     /translation="MSRFQQFLYGVRDSIPMIVGILPFGLIYGALASLAGLSLGQAVG
                     MSLLVYAGSAQFIAISLLTLGSGAVVILLTTLVVNLRHVL"
BASE COUNT         1888 a         2992 c         3248 g         2136 t
ORIGIN      
        1 caaaagtaca ctccgcttcc tcgcctcttt ttgccttgca ggactctagc tcgcgagatc
       61 gtaagtaggc tctaggtaaa acaaacaagg agcgcaatgc gctccttgtt tgttcttgcg
      121 gcaagctatt tgcgctgcca cttaccttca agctggatgt actgtcccgc cggggtcttg
      181 tcgatggctt tctgcccggc aatcgcttct acatcgctga gctggatgcc gttctgcgcc
      241 gccagcttct ggtattcggc gcggcgcgcc tggttgatca gttgggcgat ttcggcggcc
      301 tggccgccgt tactgaccac gcccagatag ccattaggca tttcgcccaa ttggccgctg
      361 gctttggcgt cgcccagggc cgacatggct tcgttaaggt tcagggctac ggccggcagg
      421 ctcaggctga gcagcagtaa caggctggca aggcgtttgt agagattcat gctcgggctc
      481 ctttagaaca atccgctgga ttcgctgaac atgctgtcca gggctttatc caccttgata
      541 tagatttcgt gttcgatctt cacattgagg ttgatgttga tcggctcatt gggcatcgcc
      601 agctgtactg tcggggtgca ggccgtgctg agcagcccca gtagcaggac ggcgaaacaa
      661 ctacgcaagc gcatggcgct tctccttggt catggctctg tcggggccgt tttggcattg
      721 cgttgcagca tacgctgttg cacacgctgc ttgatgatat cgctgacttt gtcggtcagt
      781 tgcaggctgg ccagcagggt cggaatgtcc tcttccaggt tgatattgaa gtggatcggc
      841 cggccctgtt cgatggccgg gttctgcccc tccaggcgca tggccagggc cagtttgcct
      901 tgctggtcgt aattgacctg gctgctcagg gtagtaaagc gaaaatcctc cagcgattgg
      961 gtgaccagct gcatcgccgg gttgctgcgg cccagggcgc ggatacgctc ggaatgaaac
     1021 tgcaactggc cgccgggtgc gcgcgcgctg agctggcctt gttcgatggt cacaccctgg
     1081 ctgctgatct gcagcggtaa gttgccatcc agggtgcctg tgccggctaa accttctgtg
     1141 ggatagagga taaacagctg ttcaagttcc aggccctgcg cctgtacggg aaatagcagc
     1201 ggctcggcgg ccaggctcca ttgtccgggt gccagttgca gcttgccgcc catcagcgcg
     1261 gcttcggctt ggcgtaactg cagctggcct tgggtcgggg tttgcagcgc ggcgctgtag
     1321 ttgccgctaa gttgcagcgg gcccagtggt atgccggggt tggcctgggc caggcgcagc
     1381 tcggtcaact caagctgcag ctgtttgggg tcgactcgca cagtgatacg gccatccagg
     1441 ccctccagcg cggtgcggtc ataaatcccg cccaggccct tgccgctgag ttcgagtttg
     1501 accgtgggca gctgttgatc gctggcgagg gtcaggctgg cgtcggcgtt caggcgaccg
     1561 ttgttcagct ccagcagggc tggccaggct gtgaagctgc cttttaaggg attgccgctg
     1621 cgcagaaaca gctccgccag ttgcgcttgc aggctcaggc ccttggcgct gctgtattgt
     1681 gcctgcagct tgagttgcag cccggcatcg ttgcgcagcg taccgtccag ctccgcctgc
     1741 agttggctgg cggtcacggg gccttgccag taccagcgct ggggctgcaa ttggccatgc
     1801 tgcagctgtg cactcaggtc gagggggcct tctagctgcc agtcttgcag tgtgcctgcg
     1861 gccagctggg cctgcagttt taggccgctg ctgctggcct tgagttgctg cgcttgcata
     1921 tccgtactgc gcagatgctg caggctgagc tctgcgcctt tgccgagagc cagatccagc
     1981 tgctgtgcat ccaggtaagc gtcgagttgc aactgcgtct gcagtgcgcc gattttccag
     2041 tcgtttaact gaaatgccgg ggtggatgcg ctaaggctgc tgtccagcag ttgcagcccc
     2101 cagggcaggc cattggccag gatcagcgtg ccgtttaact cgaattggct gcgcccttgg
     2161 ccacttagct gaaggtgcag gggcagggcg cgatcaacca gttgtggggc caggttgtcg
     2221 ggtgtttgcg tggcctggat tgttagctgc agcgtttcgg gatacagctc tgccggtacg
     2281 ccactgataa ggtcattggc aatctgcttg aggttgaggt cggcgttcaa gctatcagct
     2341 agccactgcc catccagcgc cttggcggat aaattgaagc tgccctgcaa ttggcctgtc
     2401 gcgggaattg gccaaggctc tggcaagttg gcgttggcct gcaactggcc gctggcctgc
     2461 tgcaactgcg ccacgttaag tgcgccaggg gcaagctgca gttgccagct tgcctttagc
     2521 tgcgctgccg ccggtgcttc aggcagtgtg cctgcagcgt tgggtagcca ttggctaagc
     2581 cagctttgca gcaccacact ttgctgcaga tcgccggtga aatcaccttg ccagagttgc
     2641 ccgacggcgt tctgttgcag gctgctgctg agctgcagtt gcggttgctg attgatcgcc
     2701 aggcttagct gcagcgctgc agttgtggcg tcgccctgca actgcgcgtg ccaactcagt
     2761 tggtcctgct ggtgctgcag attgagcagc gcatcaagct gcgctgcttg tttaagcagc
     2821 tgcagatcac ccagcagctg gcagcgtgtg tcggcgcagg gcagctcgat ctgcagcgcg
     2881 tcgatctgca cgcgttgcgg caataggctg ataatggcgg cgagttgctc aagcggcaac
     2941 gcaaaaccag tgctgtcgtc agatgctggc tcgggttgag agggcaggtt cagttgcagg
     3001 ccggtgatct gcacctgctg ccagaagggc agcgccaggc tgaatccgct ccagcgcaat
     3061 tgcacctgtt gcagctgcac tgcgccgctg gggtgctgca ggctgagctg gtcaagctgg
     3121 ataccctggg tggataggcc cagaccgcgc cattcgaggt tctcgatttg ctcgcgttcg
     3181 agcaggcggc tcaggctcag gtaggcgtaa gcgccaagca gtacgagcag taaaaccaga
     3241 caactgataa gtcgcagcca ggtgcgccgg ctgggcataa gcactgatcc atagtggggg
     3301 agttgcgtgg caggacgtta acccatgtct ggcagactgc caatcacagg ttattctgtt
     3361 ctgtgtaatt gcagcattta gtgatagttt tgttgtaaac agacagtgtg aaagtaggag
     3421 gccgtgatga gcgcattgat tcgaccggtt gccgacgccg atgcggtgct ggccaaggcg
     3481 ctgctcaata cccgtgaaca actgggcctg acccaacagg agctggcgga catcattggc
     3541 gtacaccgca ccgccatctc gcgctgggcc gagggcggct tgcgggtgca cagcaagacc
     3601 ggcgaactgg ccctgctgct gattcgcgta taccgctcat tgtttgctct gtttggcggc
     3661 aacctggcgg atatgcagca ttttctgcgc accgataacc gccatctgga tgcaccgccc
     3721 ttgtccctga tgatgcaggt gcaggggttg gtgcgggtgg tggagtacct cgatgcgatt
     3781 cgtggcaagg gttgagtcgt aaccagtaag cacagtaccc aacaataata aaagttaagg
     3841 acaacgccac catggatatc tggcaacact gccaaggccc ggcccagatc aaaccgctgc
     3901 gtggacgcct ggtgcgtctg gtcgagagcc aggctcaggt cgccactctg caactggtcg
     3961 atacccttgc cgaacaggcg ctgctcgaag agctgctgga aaccagcaag ccgccgctgc
     4021 cgcagagcgc cgaaccgctg cattacctgc tgaaaacccc gttccgttat ccaccgctgc
     4081 gctggggctc gcgctttggt tcggtgcatg agccgagcct gttctatggc gcgctgcgta
     4141 tcgacaccgc catggccgag gccgcgtatt accgttttct gctctgggat ggcatgagca
     4201 tcgcgccgcc cagcgggcgc atcctttccg agcattgcac tttcgaagca cgctatcagg
     4261 tcgaacgcgg cattcagttg cagcatccgc cgtttgcgga tttccaggcc gagctctgcc
     4321 acgccagcgc ttatcaacct tgtcaggcgg taggcgcggc catgcgcggg gcaggggtgg
     4381 aggcgttcga ataccgttcg gcgcgctgcc cgctcaaggg caacaatgtt gcgttattcg
     4441 tcccaggcgc gttggcagaa aaacgcccgc gcaacctgct gagttggctg tgcgaaacca
     4501 ccgacgacta tgtcgccttc aaaaatgccc aagtgccgga tacccccaga gtttttcgcc
     4561 tggatgccta tcaggtggat ggccgcctgc cgcgcccggc ctagacgggg cggctttttc
     4621 aaggcgcttt tgccctgaaa aagggcagcg cgacggcatg acgcagcgac ttgtgcacaa
     4681 ttgcgatgcg ccttgtcaag ccgcatgcag tgcttgacgt aaacccttga ttctttttat
     4741 tgattttata agtcaatgaa atatatggtt tttttgtgct ggtgaaaaaa tcaccagttt
     4801 gaccttgggc cccacgccat aggcgctgag agcgcttgct cccatgttat ccacgaagtt
     4861 atccacagct tctgtggatt aacccgagcg cttgctctag ctcgcggcat ggtgcttttt
     4921 gcaaggcttt accttggcga aaaaaggagt agagttgcgc gccttcccgg ctaccactgc
     4981 tttttatgcc tcgcgcaatc actcgactac ctatcgctgc aaccactgct gcccctctgc
     5041 ccacccgtgt ggcgctctgg ctactggaca gtcctcgcct cggtcatgcc cctagcgtca
     5101 aacgctttgc tggtcatctg cttaagcagc ctgctttgca gggcgtggtg caggcgcaga
     5161 gtcgactggg gcagatgctt tgtcgtgatt gcggcaaccc gcgtgatcgg cgcatgggtt
     5221 tcgagctgct gcgccaggcc gcccgcgccg gcgatatggg cgcccagctg gaactcggcc
     5281 agctgtatag ccagccgcgc aacaatgaac cgctacaggc acggcattgg ctggaactgg
     5341 ctgcggggca gggttcgcta gaagcgcggc aactgcttaa acagctctag agccagctca
     5401 aagtctgctg cgcgtcggcc ctgctgcgtt aaaaacaggc tggtgcgcca gcccggtcgg
     5461 aagccgcttg cggctagcgc gcttcagcgc gacccgaagg gtgagtgaaa cgagtaatgt
     5521 tcatgtacag ctcgtacact cgtgtgcgag cccactccgc ttcctagcct gcttgaccag
     5581 ccggaggctg ttactaacgt agcgccttgc atgacgctag ctcgcgatac tttgagcagg
     5641 ccctgacgca atgggccgct ctgtaagaat actggttata ctgccagtac ttttctgcgg
     5701 agctgcttat gcccatcgat ctgtccaccg cactggcacc tgcgttgctc ggttttgccg
     5761 ccgccgcact tcctcttcta tatatggctt ggtcgctgca acgccgcctg gccagtcagc
     5821 agctggaatt cagcctgctg caagagcgtc tgaataatgc gcagctggcc cagactggcc
     5881 tggcggcgca actggatgac tgccgagagg aaatcgccga gatcagcgag atcaaggccg
     5941 atcagcaggc cgaactggca gcgctgcgcc gggaggccga tttgctgcaa caggaacggc
     6001 agatcgcccg cgaagctgcc cagacctggg ccagtcagcg cgaacagcag gaagctgaaa
     6061 ttcgccgcct ggatgctcag cgcgcggccc tcagcgccga actcaaagaa caacaagaca
     6121 gccatcagca gcgcctgagc gacctgcaag gttcgcgcga tgagctgcgc gcgcagttcg
     6181 ccgagctggc agggaagatc ttcgatgagc gcgagcagcg ttttgctgag accagccagc
     6241 agcagctcgg ccagttgctc gatccgctca aggagcgcat ccagtctttc gaaaaacgtg
     6301 tggaagaaag ctaccagcag gaagcccgcg agcgcttttc cctgggcaag gaactggagc
     6361 gtttgcaaca gcttaaccag cgtctgggcg atgaggcgac caacttgact cgagcgctca
     6421 agggccagaa aacccaaggc aactggggcg aattggtgct ggagcgggtg ctggaacacg
     6481 ccggcctgga gaagggccgc gagtaccaga cccaggtcag cctaaagagc gccgatggcg
     6541 agcgcttcca gcccgatgtg ctgatccagc tacccggcga caagcaagtg gtggtggatg
     6601 ccaaggtcag ccttaccgct taccagcaat atattgctgc cgaggacgac gcgagtcggc
     6661 agctgtcgct caagcagcat gtgctgtcgc tgcgcagcca tttgaagggc ttgtcgctca
     6721 aggactacca gcgactggaa ggcctgcaca gcctggattt tgtgttgttg tttgtaccga
     6781 ttgaggcggc ttttgctgcg gcgttgcagg ctgatccagg cctgtttcag gaggccttcg
     6841 atcaacatat cgttatcgtc agcccgacca cgctgctggc caccttgcgg gtgatcgaca
     6901 gcctgtggcg gcaggagcgg cagagccaga acgctcgcga gattgccgag cgcgccggcg
     6961 cgctgtacga caagttcgtc gcctttattc aggatctgga tgaaatgggc gcgcgcctgc
     7021 agcaactgga caaggcctat gccggcgcgc gcaacaagct ctgcgacggg cgcggcaata
     7081 tcatcagccg ggtggaaaac ctcaagctgc tcggcgcccg tgccagcaag agcctgcctg
     7141 ccgagttgct cgaacaggcg gccggggtga tggtgctgga cgaggccagc gagtagcgca
     7201 tctatagcca cctgtaggcg cggctgggcg gcactccgtc atgcccgcga gccgtgctga
     7261 cggattattt cgcgactacg aactgggcgt tcccgccgct cttgcgggtt acgcgcttat
     7321 tggtcctgtc tcggcagccg cttgggtgtg tgcagtgaac tctcgtagcc aggctgccag
     7381 ttgctgcggt tcggccagca gctgccagtg cccagcgttc gcctcgcgcc gttgcagcgt
     7441 cggcgcccaa tgctgcagct ggtcgaacag ctgcggccgc acgaagcggt cgcgggtcgg
     7501 cacgatcagt tgcactggca cttgggtgtg acgcaggcgc ggctggaaca ggctgcggat
     7561 aaagttggcg cggtacagct tgaccccgtg cacgccgtcg gagcgttggc tgctgctgat
     7621 tggcacattg cgcagccctt cgaccttgtg cagcagctgt ggccaggcgc ggtccaggcc
     7681 caggcgccag gtcagctccg gcagcagtgg ggtgtgaaac agggcgatat accaggagct
     7741 gagcagttgc ccgagtacct gcagcagcgc gcgcggagtc ttctgtttga gccggtcgcg
     7801 catccagtgg ccgacatggt ccaggcaggg cccggagatg ctggtgtagg aggccagcag
     7861 tggctgaatg cgcggttcgg tgaccgcctc ccaggcctga attgagcccc agtcatgggc
     7921 taccagatgc accggctgct cggggctgat ggccttgatc actgcttcca ggtcattggc
     7981 cagtttctcc aggtgatagt ccaggcgctt gcgtggaata tccgatgcgc caaagccgcg
     8041 cacgtcgtag gcaatgatct ggtaatcgcc tgccagctcg cgcaccagag gcaaccagat
     8101 ttcgtggttg tccggataac cgtgtaccag tagtagagtt gggccgtttg ctttgcccca
     8161 acgataggcc gccagctgca cgccatcgcc cttaacccac agctgttcag cctgcagctc
     8221 tttatatggc agcactgctg gcgcgttcat gcaaacaccc ggtgcaaggt gagcagcggt
     8281 gagtggcgct gggtgatata cgcgaggtcg atgggcatac ggcgtctccg agttgtcatg
     8341 gctgaccgct acatcagttg atgtcacggt tattgttggg cgctcggagc gtatctgcct
     8401 agccatcgtc aggggtaact gcagggcttt atgcggtgtt actgacttgg ctggaggcct
     8461 ttgggaaaag ggctttgcgc aggtttagcg agattgcctg ctgaggttgg cggagcaagt
     8521 ccagcccttg gttggggtaa tcaagccatg gggagtcgat ggatgaactg ttgcacggct
     8581 gtttgtgttc cgccgattta atagcctgct aaagcaatag taatactgcc gcaccagcca
     8641 gcagaccggc agccatcacc ggcagggtca tcagtgccag tttgcggcca ccgatcaggg
     8701 cgaccagaaa gcctttgacc aggctgttgc tcagcgcggc gagaaagatc ccctgcaccg
     8761 ccacctgtgc acccagttcg cccttggcgc tacgcgccag cgacagggta atggcatcca
     8821 catcggccag acccgcgagc agcgccacca gataaacgcc ggcatcgccg agccagtgct
     8881 gtgcggcttc gaccattaat agaatcaacg ccagcaacgc ggcaaaacgc agcgccgggc
     8941 ccagctcgaa ggggtttttc agcggtggtt cggcctgttc ttcgctctcc gtgccagcgc
     9001 ggcggaagta cagcagcgcg ccacccgcgt ataccagggt ggcaatacct aaaggccagg
     9061 ccagttgcgg cagcaggctg gcgttgacca ggccgacttc cagcaacacc cgtggaaaca
     9121 tcagtgccga ggtggcgagc aggccgcagg ctaggattgc ttgcagattg cgaccctggg
     9181 ccaggcgcgc cagggttacg gtcatcgccg tggacgacac cacgccgccg agcagcgcgg
     9241 tgatcagcag gccatgacgc gtgcctacta gacgaatcgc cacataggcg gcaaagccga
     9301 tactggcgat caataccacc atccaccaga ttgcgtaggg gttgaaggct tgccacgggc
     9361 cgtaaccctg attgggcagc gctggtagca acaccaggga aataaacagc agcttcagtg
     9421 cgccagacac ttccgcctcg ctgagccgct gcagggcgcc gtgcagggat tgcttgaggc
     9481 ttagcagcaa tgccaccgcc actgccccgg ccgccgccag cccggcataa ccggccatgg
     9541 ccaggctacc gagcagaaag gtgatcaaca gcgccacctc gcttgtcagg cccagatcgc
     9601 ccaggcgctg cagttctcca aagtaggagg ccaccaccaa tagggcaaag ccgctgaaga
     9661 tcaccgccca ggccactacg ccgaagtgtt cacccagcag catggcaaag gccccgagca
     9721 ggctgctcag ggcgaaggtg cgaattcctg cgaccagctg cgtgtcctcg gtattgcgtc
     9781 cctgccagcc gcgttctgca ccaatcagca ggccggcggc cagggcgctg gcgaagttca
     9841 gcaaggtgtc gagggcttcc ggcatgagca gatcctgggt gggcaatgct ggcagtctag
     9901 ccagtgggcg ttgggctgta ttgcggcagg tcagccagcg gcgttattgc tgcgcaagat
     9961 tgttcaagac gctgcggccg cgatgcgcca ggctatgcag caaaggaggg cgcatgagcc
    10021 gttttcagca atttctctat ggggttcgtg acagcattcc gatgattgtc gggattctgc
    10081 cgtttggtct gatctatggc gcgttggcca gtctggctgg gctgagcctg gggcaggcgg
    10141 tcggtatgtc cctgctggtg tatgccggtt ccgcgcaatt tattgccatc agcctgctga
    10201 cactgggcag cggcgctgtg gtgattctgc tcaccacgct ggtggtcaat ctacgtcatg
    10261 tgct
//
LOCUS       DASRUM010000197         9287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_437798, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000197 DASRUM010000000
VERSION     DASRUM010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9287
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437798"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1036)
                     /locus_tag="VFF22_12940"
     CDS             complement(2..1036)
                     /locus_tag="VFF22_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX17752.1"
                     /translation="MMKSLKLAALAAGVACAMPALAVDLTAISFGGASKDAQAKAFYE
                     PFEKATGNKILAGEYNGEMARVKAMVDTNSVSWNLVEVESPELARGCDEGLFETLDSA
                     QFGDQNNFVKGAIQPCGVGFFVWSTVLAYNADKLAKAPTGWADFWDVKQFPGKRGLRK
                     GAKYTLEFALMADGVAPAEVYKVLATRDGVDRAFRKLDELKPHIQWWEAGAQPPQYLA
                     SGDVVMSSAYNGRIAAVQAESNLQVVWTGGIYDFDSWAIPRGAKNQELAKEFIAFSVQ
                     PEAQKVFSENIAYGPANKQAIDLLAPERLNVMPTTPENIKDQVAIDVVFWADYGEQLE
                     QRFNAWAARN"
     gene            complement(1081..2190)
                     /locus_tag="VFF22_12945"
     CDS             complement(1081..2190)
                     /locus_tag="VFF22_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX17753.1"
                     /translation="MSGSQANDVLVSFRGVQKSYDGENLIVKDLNLDIRKGEFLTLLG
                     PSGSGKTTSLMMLAGFETPTAGEILLDGRSLNRLPPHKRDIGMVFQNYALFPHMTVAE
                     NLAFPLSVRGLSRTDSAERVKKALSMVQLVSFLNRYPGQLSGGQQQRVALARALVFEP
                     QLVLMDEPLGALDKQLREHMQMEIKHLHQRLGVTVVYVTHDQGEALTMSDRVAVFHQG
                     EIQQIADPRTLYEQPSNTFVANFIGENNRISGQLISRDGERCVVGLARGEQIEALAVN
                     VGALGESVTLSIRPERVRLNGHSDACRNRFSGRVAEFIYLGDHVRIRMEVCGKQDFYI
                     KQPVAELDASLAVGDLVPLGWHVEHARALDPLQAD"
     gene            complement(2424..3053)
                     /locus_tag="VFF22_12950"
     CDS             complement(2424..3053)
                     /locus_tag="VFF22_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZX17754.1"
                     /translation="MIRVLVAEDHAIVREGIKQLIGMARDLEVVGEASNGEQLMETLR
                     KVECDVVLLDISMPGVNGLEAIPRIRALLRPPAILVLSMHDEAQMAARALKIGAAGYA
                     TKDSDPALLIAAIRKVASGGRYIDPELADRMVFEVGLTDSRPPHAQLSEREFSVFERL
                     VRGEGVNDIALHLAVSNKTVSTHKARLMQKLGLQSVADLVRYAMEHKLL"
     gene            complement(3050..5446)
                     /locus_tag="VFF22_12955"
     CDS             complement(3050..5446)
                     /locus_tag="VFF22_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZX17755.1"
                     /translation="MRLLIILLFWPALLCAAESNLLPLRPAFNAEQREWLSAHDTLRV
                     GLVMQPPWAQFDRRQQRLSGANVELMNRLLQGMGVQGQWLRFADQGALEAALQRGEVD
                     LAPGLQQTPQGLRRWRFSDPYLRVPHLVVGERRGSSAVDLDRIGRDEKVAVRAPSAVQ
                     EYLRVTHSNVQLQPVANEREALDLLLRQEVSYAVVDEARLSLLLRETQFAGLSIVGDI
                     GLPQLLRIGSRRDAPLLADILQRTLQTLPGGELEQLQTRWFPLTTRQLGNPLAFWKGF
                     SLFLGIILLTLAAIVRYLLLQRRSLERQLLEARAVLSQREAAEQALRLSQFSIDNSTV
                     GILWVNWDSHVRYANLAAERMLGYGRGALVDMPLVQLEPALSMDRWLSLWRQARGSLE
                     PLPGFEIDCQRADGSRLPVDVSLSFLRFDQSEYLVVFITDVTERRRAQAALQESEARL
                     KGIAGNVPGLVFRLERASAEQPVEFAYISEASLDLVGYPAAELQREDRGIRSLVHPED
                     QARYHQTQDAALLGDCDWQWQGRILTRDGELRWADIKATARSLGEGRVVWDGIVWDIT
                     ENKQVELALAESRAHLRELAAHLESVREEEKARIAREVHDELGQVLTVLKLETAMCEL
                     AFAGQVEGLDERLTSMKKLIANLFQLVRDVATALRPPILDAGIASAIEWQVRRFESRT
                     QIPCLVEVPDNLPPLSDAKAIGLFRILQEALTNVMRHAGAHSVQVYLRVEQGELCLCV
                     IDDGKGFTQEPRKAGQSFGLVGMQERVFMLGGRLQIDSQPGEGTTLLAHVALDEEALT
                     "
     gene            5732..6766
                     /locus_tag="VFF22_12960"
     CDS             5732..6766
                     /locus_tag="VFF22_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HZX17756.1"
                     /translation="MRSFIPTRPTLFSLLLALLLIQPLQAEESDSAATEDSEEAVVET
                     PRAPLAERSEVEATALEQQLEQKEQQQLQAGDERFLALWLPANVAEPSGVVIILPGDD
                     ETADWPQSVGPLRSKLPNGGWHSLSLTLPDPNSDAPLLRSAEPATTPPTEEATADATE
                     APKEDAENTPATDEAAAQTSTSQLDSAETAASKVKTIEEQQKAHAERVLARIESAIGF
                     AEQQQAKTVVLLGHGSGAYWAAHYLAERKPANIQNLLLVAAQLPAGYTPPLDELIPQL
                     QLATGDFYYKDLPADRQAALKRSQASKRQKHPSYIQIAMKALPGNREAEQEQLYRRIR
                     GWLTLKLQAK"
     gene            complement(6770..7531)
                     /locus_tag="VFF22_12965"
     CDS             complement(6770..7531)
                     /locus_tag="VFF22_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007993213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HZX17757.1"
                     /translation="MIWPATLLGAVAGLAVASIPGAMLGGLLGQVLDRRLRLQSWAAL
                     RERLGGRADVRDEWLLFVLLGRLAKSGGRVLPAHIQQAQAEMQRLGLDAEGQRQAIAA
                     FARGKTGRDSLRGPLRSQRARSEVLLRACWRMAWVDGQVGQAERELIMLWGKWLQVSS
                     ATQAQLSNAYAPQQGPLVSTASNAYQRALNLLNVNADAEPAQIKQAYRRLLSRHHPDK
                     LAGSGATPERVHEATERTRELHQAYDLIRQRHGFR"
     gene            complement(7533..8204)
                     /locus_tag="VFF22_12970"
     CDS             complement(7533..8204)
                     /locus_tag="VFF22_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZX17758.1"
                     /translation="MKAMILAAGKGERLRPLTLHTPKPLVRAAGVPLIEYHVRALAAA
                     GFAELVINHAWLGQQIEDYLGDGSRFGISIAYSAEGEPLETGGGIFQALPLLGDQPFV
                     LINGDIFTDYPFAELRRPLTGLAHLVLIENPGHHVKGDFCLREGQVTDAQAGQDSLTY
                     SGMAVLSPALFAGCQSGVFSLAPLLRAAMATGQVTGERFDGCWVDVGTHERLAQVERL
                     LEARG"
     gene            complement(8201..9217)
                     /locus_tag="VFF22_12975"
     CDS             complement(8201..9217)
                     /locus_tag="VFF22_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009051157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZX17759.1"
                     /translation="MPDEDVRLKLLENWLDQQLPGLFAEQGWGVVPAATLTSASSDAS
                     FRRYFRWQGAGRTLIVMDAPPPQEDCAPFVKVAHLLAEARLNVPQILAADLERGFLLL
                     NDLGRQTYLDVINEGNADALFADALQALLAFQQLPMTTPLPSYDDALLRRELQLFPEW
                     YAQRHLGVEFSEQQQTMWQRASQLLIDSALAQPKVLVHRDYMPRNLMLSEPNPGVLDF
                     QDAVYGPVTYDVTCLFKDAFLSWPEARVRSWLEQYWQQAREAGVPVQPVLEDFLRASD
                     LMGVQRHLKVIGIFARICHRDGKPKYLGDVPRFFSYIEAVLERRPELAELAELLRSLR
                     QESV"
BASE COUNT         1993 a         3070 c         2600 g         1624 t
ORIGIN      
        1 ctcagttacg cgcggcccaa gcattgaagc gctgctccag ctgctcgccg tagtcggccc
       61 agaacaccac atcgatggcc acttggtcct tgatgttctc cggggtagtc ggcatcacgt
      121 tcaggcgctc aggggccagc agatcaatgg cttgtttgtt ggccggaccg taggcgatgt
      181 tctcggagaa gactttctgc gcctcgggct gtaccgagaa agcgatgaat tccttagcca
      241 gctcctggtt tttcgcacca cgcgggatgg cccaggagtc gaagtcataa ataccgccgg
      301 tccacaccac ttgcaggttg ctctcggctt gtacagcagc gatgcggccg ttgtaggccg
      361 agctcatcac cacgtcaccc gaggccaggt actgtggtgg ctgtgcgccg gcttcccacc
      421 actggatatg cggcttgagc tcatcgagct tacggaaggc acgatcaacc ccatcacggg
      481 tcgccagcac cttgtacacc tcggccgggg ctacgccgtc cgccatcagg gcaaattcca
      541 gggtgtattt agcgccctta cgaaggccac gcttgccagg gaattgctta acatcccaga
      601 agtccgccca gccagtcggg gccttggcca gtttgtcggc gttgtaagcc agcactgtgg
      661 accaaacgaa gaagcccacg ccacagggct gaatcgcgcc tttgacgaaa ttgttctggt
      721 caccaaactg tgcagaatcg agggtctcga acaggccttc gtcgcagcca cgggccagtt
      781 ccggcgactc cacttccacc aggttccagc tcacgctgtt ggtgtcgacc atggctttga
      841 cacgggccat ttcgccgttg tactcaccgg ccagaatctt gttgccagtg gccttttcaa
      901 agggttcata gaacgccttg gcctgcgcat ccttgcttgc gccaccaaac gagatggcag
      961 tcagatccac cgccagggct ggcattgcac aggccacgcc agcggccagg gccgcgagct
     1021 tcagggattt catcattgtt gttagctcct ctgtgtcact tgggacgggt tagtgttcgg
     1081 tcagtcggcc tgcagtgggt cgagagcgcg ggcatgctcg acgtgccagc ccaacggcac
     1141 cagatcgccc accgcgaggc tggcatccag ctcggccacg ggctgcttga tgtagaaatc
     1201 ctgtttgccg cacacctcca tgcggatgcg tacgtggtcg cccaggtaga tgaactcggc
     1261 cacacgcccg gagaaacggt tgcggcaggc atcgctgtgg ccattgaggc gcacgcgctc
     1321 gggacgaatc gacagggtta cggactcccc cagcgcaccg acgttgaccg ccagcgcctc
     1381 gatctgctca ccacgggcca ggccaaccac gcagcgctca ccgtcgcggc tgatcagctg
     1441 gccgctgatg cggttgttct caccgataaa gttggcgaca aaggtgttgc tcggctgctc
     1501 ataaagggtg cgcgggtcgg cgatctgctg gatctcgccc tggtggaaca ccgccacgcg
     1561 gtcagacatg gtcagcgctt cgccctggtc atgggtgacg tacaccacgg tcacgcccaa
     1621 gcgttggtgc aggtgcttga tctccatctg catgtgctca cgcagttgtt tgtccagcgc
     1681 accaaggggc tcatccatca gcaccagctg cggttcgaac accagagccc gcgccagcgc
     1741 cacacgctgc tgctgaccac cggaaagctg gccggggtag cggttaagga agctgacgag
     1801 ctgcaccatg gacagcgctt tcttcacccg ctcggcgctg tcggtacggg acaggccgcg
     1861 cacactgagc gggaacgcca ggttttcggc caccgtcatg tgcgggaaca gggcgtagtt
     1921 ctggaacacc atgccgatat cacgcttgtg cggcggcagg cgattgagcg agcgaccgtc
     1981 gagcagaatc tcgccggcag tgggggtttc gaagccggcc agcatcatca gactggtggt
     2041 tttacccgaa ccggacggcc ccagcagggt gagaaattcg cccttgcgga tatccagatt
     2101 gagatctttg acgatcaggt tttcgccgtc gtagctcttt tgcacaccac gaaagctcac
     2161 caatacatcg tttgcctgag acccgctcat cgccacaccc ttgttgttgt ctgcacgctg
     2221 atggcacaag actagggggg ttaaaccggg gacataatca ggctggatga caacctgatg
     2281 tcaggcggca ggagaacctg ctgtagggat cgccctacaa aaaatggaag ctgcgacagc
     2341 cgcacaagaa cagtgcgcaa gccacgtcag tcgggcctta caggccgtag agcaacacgt
     2401 caaaaagcac gccaagcacc gctttagagc agtttgtgct ccatggcgta gcgcaccagg
     2461 tcagccacag attgcaaccc aagcttctgc atcagacgcg ccttgtgggt gctgaccgtc
     2521 ttgttgctga ccgctaggtg cagggcaatg tcgttgaccc cttcgccacg caccaggcgc
     2581 tcaaacacgg aaaattcgcg ctcggacaac tgcgcatgcg gcggacgcga atccgtcaga
     2641 cccacctcga agaccatgcg gtcggccaac tcagggtcga tataacggcc accactggcg
     2701 accttgcgaa tagcggcaat cagcagtgcc ggatcgctgt ccttggtggc gtaaccggcc
     2761 gcgccgatct tcagagcacg cgctgccatc tgcgcttcat cgtgcatgga caacaccaga
     2821 atcgccggtg ggcgcagcag cgcacgaatc cgtggaatgg cctcaaggcc gttgacgccg
     2881 ggcatggaaa tatccagcag caccacatcg cattccacct tgcgcagggt ttccatcagc
     2941 tgctcgccat tgctcgcctc gcccaccacc tcgaggtcgc gcgccatgcc aatcaactgt
     3001 ttgatccctt cacgcacgat ggcgtggtct tctgccacca atacccgaat cacgtcagtg
     3061 cctcctcatc caatgctaca tgcgccagca gcgtagtgcc ctcgcccggc tgactgtcaa
     3121 tctgtaaacg cccgccgagc ataaataccc gctcctgcat gccgaccagg ccgaacgatt
     3181 gccctgcttt gcgtggctcc tgcgtaaagc ctttaccgtc gtcaatcaca cacaggcaca
     3241 actcaccctg ctccacgcgc aagtaaacct gcacgctgtg cgcaccggcg tgtcgcatca
     3301 cattggtcag tgcctcctgc agaatgcgga acaggccaat ggccttagca tcgctgagcg
     3361 gcggcaggtt atcgggcacc tccaccaggc aaggaatctg ggtgcgcgat tcaaaacggc
     3421 gcacctgcca ttcgatggcc gaggcaatgc ccgcatccag gatcggcggg cgcaacgccg
     3481 tggccacgtc gcgcaccaac tggaacagat tggcgatcag cttcttcatg ctggtcagac
     3541 gctcgtccag cccctcgacc tggccagcaa aggccagctc acacatggca gtttccaact
     3601 tgagcacggt cagcacctgc ccgagctcat cgtgcacctc gcgggcaatc cgggcttttt
     3661 cttcctcgcg gacgctttcc aggtgggccg ccagctcgcg cagatgggcg cgcgactccg
     3721 ccaaggccag ctcgacctgt ttgttttccg tgatgtccca gacgatgccg tcccacacca
     3781 cccgcccttc ccccaggctg cgcgcggtgg ccttgatgtc cgcccagcgc agctcaccgt
     3841 cgcgagtcag aatacgtccc tgccattgcc agtcgcagtc gcccagcagc gctgcatcct
     3901 gggtctggtg atagcgcgcc tgatcctcag ggtggaccag gctgcggatg ccacggtctt
     3961 cgcgctgcaa ctcggccgcc gggtagccca ccaagtccag gctggcttcg ctgatgtagg
     4021 caaactccac cggctgctcg gcgctggccc gttccaggcg aaacaccagc cccggcacat
     4081 tgccggcaat gcccttcagg cgcgcctcac tttcctgcaa ggcagcctgc gcgcgtcgcc
     4141 gttcggtgac atcggtgata aacaccacca ggtattccga ttgatcaaag cgcagaaaac
     4201 tcagcgacac gtccaccggc aagcggctgc catcagcccg ctgacagtcg atctcgaaac
     4261 ctggtaaagg ctccaggctg ccacgtgcct gacgccacaa gcttaaccag cgatccatgc
     4321 tcagcgcggg ttccaactgt accaacggca tgtcgaccaa cgcgccacgg ccatagccga
     4381 gcatgcgttc agccgccaag ttggcgtagc gcacgtggct gtcccaattg acccagagaa
     4441 tgcccaccgt gctgttatca atcgaaaact ggctcaagcg cagcgcctgc tcggcggcct
     4501 cacgctggct gagcaccgca cgcgcttcca gcagctgtcg ctccaagcta cggcgctgca
     4561 gcagcaggta ccgcacaatg gcagccagcg tcagcaggat aatgccgaga aataggctaa
     4621 agcctttcca gaacgccagc gggttgccca gctgccgtgt tgttagggga aaccagcgcg
     4681 tttgcagctg ttccagctca ccaccgggca gagtttgcaa tgtgcgctgg agaatatcgg
     4741 cgagcagcgg cgcatcgcgg cgactgccga tgcgcaacag ctgcggcaag ccaatgtcac
     4801 cgacgatgct cagcccggca aactgggttt cacgcagcaa taagctcagg cgcgcctcat
     4861 ccaccaccgc gtaactgacc tcttggcgca acaacagatc cagcgcttcg cgttcattgg
     4921 ccaccggctg caactgcaca ttgctgtgcg tcacgcgcag gtattcctgc acggcactgg
     4981 gagcacgcac ggcgactttt tcatcgcgcc cgattcggtc aaggtccacc gcactgctgc
     5041 cgcgtcgttc gccaaccacc aaatgcggca cacgcaaata cggatcagaa aaccgccagc
     5101 gtcgcagccc ttgcggggtt tgctgcaggc caggagccag gtccacctca ccgcgctgca
     5161 aggccgcctc cagcgcgcct tgatcagcaa agcgcaacca ctgcccctgc acgcccatgc
     5221 cctgtagcaa gcggttcatc aactcaacat tggcccccga caggcgctgc tggcggcgat
     5281 caaactgcgc ccagggcggt tgcatcacca ggccgacccg caaggtgtca tgcgcactca
     5341 gccactcacg ctgttcagca ttaaacgccg ggcgcaatgg caacaggttg ctttcagcgg
     5401 cgcacagcag cgcgggccaa aacaaaagaa taatcaacag acgcatcaag caaccacact
     5461 tcattagcaa gccgctgaaa aacgtaggcg aggtcggcaa gacaaggcaa acacaggcaa
     5521 ggaagcggac tggctagcct ccgagcctgt ttttaacgca gcattgccaa cgcaggtagt
     5581 ttttcaacgg actgttaggg gtgaacaacc aagccaaggt atggcgacct tctaccttac
     5641 tccagtcctg aagggcttag agctattcag tcctgacaaa ggcacgccca ggtattaggc
     5701 ttcaattgcc tgcccccata aggagtgcct catgcgcagc tttattccca cacggcccac
     5761 ccttttcagc ctgctattag cattgctgct gattcagccc ttgcaggctg aagagagcga
     5821 cagcgcagcc actgaagaca gcgaagaggc cgttgtggaa acaccgcgcg cgcccttagc
     5881 cgaacgcagc gaggtggaag ccacggcgct ggaacagcaa ctggagcaaa aagaacagca
     5941 gcaactgcag gcgggcgatg aacgctttct agcgctctgg ctgccggcca atgttgccga
     6001 gcccagcggg gtggtgatta tcctgccggg cgacgatgaa actgccgatt ggccgcaaag
     6061 cgtcggcccc ttgcgcagca agctgcccaa tggcggctgg cacagcctca gcctgaccct
     6121 gcccgaccct aacagtgacg caccgctact gcgcagcgcc gagccagcga caacaccacc
     6181 aacagaagaa gcaacagccg acgccacgga ggcgccgaaa gaggatgccg agaacacccc
     6241 cgcgacagat gaggccgctg cacaaaccag cactagccag ctcgacagcg ccgaaactgc
     6301 ggcaagcaaa gtgaaaacca tcgaagaaca acagaaagcc catgcagaac gcgtattggc
     6361 gcgcatcgag tcggccatcg gctttgccga gcaacagcaa gccaagaccg tcgtactcct
     6421 cggccatggc agcggcgcct actgggccgc gcactacctg gctgagcgca agccagccaa
     6481 tatccaaaat ctgctactgg tcgccgcaca attacccgcc ggctataccc cgccgctgga
     6541 cgaactgatc ccgcagctgc aactggccac cggcgacttc tattacaagg acctgcccgc
     6601 agaccggcag gccgcactca agcgctcgca ggccagcaaa cggcagaagc accccagcta
     6661 tatacagatc gccatgaaag ccctgccggg caaccgcgaa gccgagcagg agcagctcta
     6721 ccgccgcatt cgtggctggc tgacgctgaa gctgcaggcg aaatagagcc tagcgaaaac
     6781 cgtggcgctg acgaatcagg tcataagcct gatgcagctc acgggtgcgc tcagtcgcct
     6841 cgtgcacacg ctctggcgtg gcgccgctac ccgccagctt gtcagggtga tgacggctca
     6901 atagacgccg atacgcctgc ttgatctgcg caggctcggc gtcggcattt acattcaaca
     6961 aattcagggc acgttgataa gcgttgctgg ctgtcgaaac cagtggtccc tgctgcggcg
     7021 cgtaggcgtt actcaactgc gcctgggtag ctgatgaaac ctgcagccac ttaccccaaa
     7081 gcatgatcag ctcgcgctcg gcctgaccga cctggccgtc gacccaggcc attcgccagc
     7141 aggcacgcag caacacttcg ctacgcgcac gctgactacg caaggggcca cgcaagctgt
     7201 cgcgcccagt cttgccccga gcaaaagccg ctatcgcctg tcgctgccct tcggcatcca
     7261 accccaatcg ctgcatttcc gcctgcgcct gctggatatg cgctggcagc acgcgaccgc
     7321 cgctcttggc caagcgcccc agtagcacaa atagcagcca ctcatcacgc acatcggcgc
     7381 gaccgccaag gcgttcacgc aaggccgccc aggactgcag gcgcaagcgc cgatccagta
     7441 cctggcccag taaaccgccg agcatggcgc ccggaatgct ggccacagcc agcccggcaa
     7501 cagcccccag cagggtcgca ggccaaatca tcctagccgc gcgcctcaag taaacgctcg
     7561 acctgcgcca accgctcatg ggtccccaca tccacccaac aaccgtcaaa ccgctcacct
     7621 gttacctgcc cagtcgccat agccgcgcgc agcaacggcg caagcgagaa cacgccagac
     7681 tgacaaccgg cgaacagcgc aggcgataac acggccatgc cgctgtaggt caggctgtcc
     7741 tgccccgctt gcgcatccgt gacctgcccc tctcgcaaac agaagtcgcc cttgacgtga
     7801 tggccagggt tttcaatcag caccagatgc gccaggccgg ttaacggacg gcgcagctcg
     7861 gcaaaggggt agtcggtgaa tatatcgcca ttgatcagca caaagggctg gtcacccagc
     7921 aaaggcaacg cctgaaagat cccgccgccg gtttccaatg gctcaccttc agcagaatag
     7981 gcaatgctga tgccgaaacg cgatccatcg cccaggtaat cctcaatctg ctgccccagc
     8041 caggcatgat tgatcaccag ctcggcaaac ccggccgccg ccaacgcgcg cacgtggtat
     8101 tcgatcaagg gcacgccggc cgcacgcacc aagggttttg gggtatgcag ggtgagtggg
     8161 cgcagccgct cacctttgcc ggcggccaga atcatcgcct tcatacagac tcctgtcgca
     8221 gactgcggag caactcagcc agttcggcca attccggccg acgttccaat acagcctcta
     8281 tataagagaa gaaccgcggc acatcaccta ggtacttggg cttgccatcg cgatggcaaa
     8341 tgcgcgcaaa gatgccaatt accttgaggt ggcgttgcac gcccatcaag tcgctggccc
     8401 gcagaaaatc ttccagcacc ggctgaaccg gcacacccgc ctcacgtgcc tgctgccaat
     8461 actgctccag ccagctgcgt acgcgcgcct cgggccagct gaggaaggca tccttaaaca
     8521 ggcaggtgac gtcataggtc accgggccgt agaccgcatc ctgaaaatcc agcaccccag
     8581 gatttggctc actgagcatc aggttgcgcg gcatatagtc acggtgcacc agcaccttag
     8641 gctgcgccag tgcactatcg atcaatagct gactggcgcg ctgccacatg gtttgctgct
     8701 gctcgctgaa ctcgacgccc aagtgacgct gcgcatacca ctccgggaat agctgcagct
     8761 cacggcgcag cagtgcgtcg tcataactgg gcaatggtgt ggtcatcggc agctgctgga
     8821 aggccaacag cgcctgcagc gcatcggcaa acagcgcgtc cgcattgcct tcgttaatca
     8881 catccagata agtctgccgg cctaaatcgt tgagcaataa aaagcctcgc tcaaggtcgg
     8941 cggcaagaat ctgcggcaca ttgagacgcg cctcagccag caaatgcgca actttgacga
     9001 agggcgcgca gtcttcctgc ggcggcggtg catccatcac gatcagagtg cggccagccc
     9061 cttgccagcg aaaataacga cgaaaactgg cgtcactgct ggcagaggta agcgtggcgg
     9121 ccggtacaac gccccaaccc tgctcggcaa acagcccagg cagctgctga tccagccagt
     9181 tttcgagaag ttttaagcgt acatcttcat caggcattac aaggtctccg acggcgctag
     9241 ccgttgcgcg ggtcatgctt tattatccag catcttttca agcccat
//
LOCUS       DASRUM010000198         3864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1931268, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000198 DASRUM010000000
VERSION     DASRUM010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1931268"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1131)
                     /locus_tag="VFF22_12980"
     CDS             complement(28..1131)
                     /locus_tag="VFF22_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HZX17760.1"
                     /translation="MSQSTQGAQPQSRAPQSIGFLLLDNFTLISLASAIEPLRMANQL
                     SGKELYRWHTLSQSGLPLCASDGLQITPDASMATAPRLDAVIVCGGVDIQHSVTREHV
                     QWLQSQARHGAQLGGVCTGSWALAKAGLLDGFDCSVHWECLASMQEAFPRAAVSTRLF
                     SIDRNRHTSSGGTAPMDMMLHVIGREHGRELAAAISEMFIYERIRNEQDHQRVPLKHM
                     LGTNQPKLQEIVALMEANLEEPIDLDQLALYVDVSRRQLERLFQKYLHCSPSRYYLKL
                     RLIRARQLLKQTSMSIIEVASVCGFVSTPHFSKCYREYFGIPPRDERAGQQGGNVVAL
                     LPMPEDLMRPSPSSAMAALTRAQGESTFASVRL"
     gene            complement(1610..2989)
                     /locus_tag="VFF22_12985"
     CDS             complement(1610..2989)
                     /locus_tag="VFF22_12985"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HZX17761.1"
                     /translation="MAISVFDLFKIGIGPSSSHTVGPMRAAALFSGALAERRLLERTA
                     RLQVRLYGSLSATGVGHGSDRAVIMGLMGEWPDQIDPSSIEPRIAELLASGQLQLGGR
                     VGIAFDWQCDMLLLEENLPYHPNAMTLSTFAVDGSLLHENTYYSVGGGFVVDAEQAAS
                     GQLDQDHTVLPYDFSSAEELLRLCKEHGLRVSELMMANEKAWRSEAEINSGLMRLWQA
                     MRECVEQGLQHEGILPGGLNVKRRAAKLHRSLQEMSKPNVIGSTLSGMEWVNLFALAV
                     NEENAAGGRMVTAPTNGAAGIIPAVLHYFVRFSPAVSEANVVDYLLAAAAIGILCKKN
                     ASISGAEVGCQGEVGSACAMAAAGLAEILGATPEQVENAAEIGLEHNLGLTCDPVGGL
                     VQVPCIERNAIAAVKAINAAQMALRGDGQHFISLDRVIRTMRDTGADMHDKYKETSRG
                     GLAVSAVEC"
BASE COUNT          765 a         1256 c         1140 g          703 t
ORIGIN      
        1 ccgctcctac agggtaatca gtgcggctta gagcctaacg ctggcaaaag tcgattcccc
       61 ctgcgcccgc gtcagcgcgg ccatggccga ggatggcgat ggccgcatca ggtcttccgg
      121 cattggcagc agcgccacaa cattaccgcc ctgctggccg gcgcgctcgt cgcgcggcgg
      181 gatgccgaag tattcgcggt agcacttgga aaaatgcggg gtggaaacaa aaccgcagac
      241 cgaggccact tcgatgatcg acatgctggt ctgcttgagc agctggcgcg cgcgaatcag
      301 gcgcagcttg aggtaatagc gcgacggcga gcagtgcagg tatttctgga acaggcgctc
      361 cagctggcgg cgcgaaacat cgacatacag agcgagctgg tcgaggtcga tcggctcttc
      421 caggttggct tccatcaacg cgacgatttc ctgcagcttc ggctggttgg tgccgagcat
      481 gtgcttgagc ggcacgcgct ggtgatcctg ttcattacgg atgcgctcgt agataaacat
      541 ctcggaaatc gccgccgcca gttcgcggcc atgctcgcgg ccaatgacgt gcagcatcat
      601 gtccatcggc gcagtgccgc cggaactggt gtggcggttg cgatcaatcg agaacaggcg
      661 agtgctgact gctgcgcggg ggaaggcttc ctgcatcgag gccaggcatt cccagtgcac
      721 gctgcaatca aagccatcaa gcaagccggc cttcgccagc gcccagctac cagtacatac
      781 cccgcccagc tgggcgccat ggcgcgcctg gctttgcagc cactgcacgt gctcgcgggt
      841 gacgctgtgc tgaatgtcca cgccgccgca gacgatcacc gcatccaggc gcggcgcagt
      901 ggccatgctg gcgtccgggg tgatctgcaa gccatcgcta gcgcatagcg gcaggccgct
      961 ctggctgagg gtgtgccaac ggtacaactc cttgccggac agctggttgg ccatgcgcag
     1021 cggctcgatg gcggacgcca gggaaatcag ggtgaagttg tcgagcagga gaaagccgat
     1081 ggactgcggt gcacggctct ggggttgtgc cccttgagtg gactgtgaca tcaggtaaac
     1141 cctcacgcga agcaaagcac ggtagtgagc ccctctgtcg ggtggctctt cattatggtt
     1201 ctgggactgc acaacgccag atcgatagcc tgcacaacgc aaaggtcgtg cctaaattga
     1261 ttggccgttc aactaaaaac actcacccta gcccagcact agtgccgcta ggcgttggca
     1321 ggagcgacag cagtattctg ataacgaccc taagtgcccg attgacgggc gcttgagcat
     1381 ttcggtagca ctgccgggca ggtgctttgt caccccgcta cgcagggtaa aaatggcgcg
     1441 gtaacggacc aacggtcgac ccgtattcag cagcgttgcg ctgccttttt cagtgcgctg
     1501 agcagcgatg caccgcaacc gtgcacagca ctcgctgcgc acccggattg gccgccaagc
     1561 acctgctttt aacccagcag agccgacagt cagccgatcg caaacaggct cagcattcga
     1621 cagcactgac tgccaacccg ccgcgcgagg tttccttgta cttgtcgtgc atatcggcgc
     1681 cggtatcgcg catggtgcgg atcacccggt ccagcgagat aaagtgctga ccatcgccac
     1741 gcaaagccat ctgcgcggca ttgatcgcct ttactgcggc aatcgcgttg cgctcgatgc
     1801 acggcacctg caccaggccg ccgaccgggt cgcaggttag gccaaggtta tgttccaggc
     1861 cgatttccgc agcgttttcc acctgttcgg gcgtcgcgcc gagaatctcc gccagcccgg
     1921 ccgccgccat ggcgcaggcc gaaccgacct caccctgaca gccgacttca gcgccggaga
     1981 tcgacgcgtt cttcttgcac agaatgccga tagccgccgc agccagcagg tagtccacca
     2041 cattggcttc gctgaccgcc gggctgaagc gcacaaagta atgcagcacc gctggaataa
     2101 tccccgccgc gccattggtc ggcgcggtga ccatgcgccc gccggcggcg ttttcctcgt
     2161 tcaccgccag ggcgaacagg ttgacccact ccataccgct gagggtggag ccgatcacat
     2221 tgggcttgct catttcctgc aggctgcggt gcagcttggc cgcgcggcgc ttcacattca
     2281 gcccaccggg cagaatgccc tcgtgctgca aaccctgctc gacgcactcg cgcatggcct
     2341 gccacaggcg catcaagccg ctgttgattt ccgcctcgct gcgccaggcc ttttcgttag
     2401 ccatcatcag ctcggagacg cgcaggccgt gttccttgca cagacgcaac agctcctcgg
     2461 cgctactgaa gtcgtagggc aatacggtgt gatcctgatc cagctggccg ctggcggcct
     2521 gctcggcgtc caccacaaaa ccgccgccga ccgagtaata ggtgttttcg tgcagcaggc
     2581 tgccatcgac ggcaaaggtg ctgagggtca tggcgttggg gtgatagggc aggttttcct
     2641 ccaacaacag catgtcgcac tgccagtcga aggcgatgcc gacgcgcccg ccaagctgca
     2701 actggccgct ggccaacagc tcggcgatgc gcggttcgat gctgctaggg tcgatctgat
     2761 ccggccactc acccatcagc cccatgatca ccgcacggtc gctgccgtgg ccaacaccgg
     2821 tggctgacag tgaaccgtac aggcgcacct gcaagcgtgc ggtgcgctcc agcaagcgtc
     2881 gctcggccaa tgcgccgcta aataacgccg ccgcgcgcat tggcccgacg gtatgcgaac
     2941 tggacgggcc aatgccgatc ttgaacaagt caaacacgct gatagccacg actctctcct
     3001 gcactgatgc tacgtgcagg ccatgatcag gatttcccca gcgcgcagcc gccccacacc
     3061 gactcgccca tgcctaaatc cgtcagctgg ccatccacac ggcggatcgc cggtgcggat
     3121 tttcgtaggg tgggttaggc cgcgcccgct cccggcgggc ctgcggcatt caccccatcc
     3181 atggggatcg cgcaaacaga ggccgggcaa tcaccaccgc cgccaacgcg ccgtaaccca
     3241 ccatggcggc ctaccgataa accgcaatgg tgggttacgc ggcccgaccc acactacaac
     3301 cgcagacggc aacacggttt tggcacagag cccatctccc cggatttcat ccgggctaca
     3361 ggttcacact ttcacgcgcc gcagcagcaa ttcacgacac cggcgttcta cttgcgacct
     3421 cacgcatagg cagcgcagga aatctgtcgg catgatcgcc gcgcccatgg atcggccagc
     3481 cgctgctcgc cctgcgcgcc ctgtcactaa aaaacctgga gatggaaagc gacctgatgg
     3541 gcgccactct caataaaaac cgccgaaaat cttgcctgtg gaaattaatt tgtcattttt
     3601 ttcgaacaac gcgcagcccc ctgctcatcg tgcctgtggc catttcaaaa gctgcaaacc
     3661 ccggccggcg tggcttgcag ctttggccaa gaggcgaaag agtctgttat ggttttttta
     3721 gtcaaagctc atgtcgccgc gcaacacgtc agcgtcgcaa accgacaatt gcgacaggcc
     3781 gaagactata tcgttgagtc agccgatttc gtcgctgtcg ccttagcagg gtcgacagac
     3841 cgggcgccca cagccccgga ccga
//
LOCUS       DASRUM010000199        22826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1104247, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000199 DASRUM010000000
VERSION     DASRUM010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 22826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22826
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104247"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..742
                     /locus_tag="VFF22_12990"
     CDS             35..742
                     /locus_tag="VFF22_12990"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZX17762.1"
                     /translation="MKQHRLAAAVALVGLVLAGCDSQTSEITLETPAQKASYGIGLNM
                     GKSLAQEGMDDLDSDAVAQGIEDAIGKKEQRLKDEELIEAFAFLQKRAEERMVTLNEE
                     SAKAGKKFLEDNAKREGVTTTASGLQYEVVKKADGAQPKATDVVTVHYEGKLTDGSVF
                     DSSVERGSPIDLPVNGVIPGWVEGLQLMHVGEKYKLYIPSELAYGEQSPTPAIPANSV
                     LVFDLELIAIKDAATQE"
     gene            1113..1568
                     /locus_tag="VFF22_12995"
     CDS             1113..1568
                     /locus_tag="VFF22_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003257348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HZX17763.1"
                     /translation="MKTPKNFARYQFLAKRFLKAGRLPALLLSVARKREKLGNGFAEL
                     AEQLKLLQALCLAWWRGEYRAIDSRALLAVVAALLYFVTPLDAIPDWLFGIGLVDDLA
                     VLAWVLRTWQEELAVFQQWRAAQAPEVLRVVERLPELDESLAPLHGRVE"
     gene            complement(1580..2302)
                     /locus_tag="VFF22_13000"
     CDS             complement(1580..2302)
                     /locus_tag="VFF22_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX17764.1"
                     /translation="MRTLITMLLIGCSGWACADELRWGFATADGMPYVDVHEQQLRGG
                     VIYKLGQLASQQLGYQAAFVETPNKRIDEFMQRGRIHVICNSNPQWIDNPERYHWSAP
                     LYREEDVLLLHRQHAPINSLHELYGKTIGTQLGYVYSSELMAAFASKQIIRQDLRDHS
                     AGLNLLRKQRLDAIIDMRRPLRFQLTKRADAPLQINPWVIERYDMHCTYSPQLPVSAE
                     LLDRTLQQLRDQGLIEQMLNDI"
     gene            2437..2757
                     /locus_tag="VFF22_13005"
     CDS             2437..2757
                     /locus_tag="VFF22_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZX17765.1"
                     /translation="MNVQVIERDGQPEYAVLPWAQYQALLQAAGRAPEVAAVAVDASK
                     PALSQLATLREAKGMSAEQLARNVGISPHYLSMIESGERQPDAAIQRALAWHLGVDGW
                     EAQP"
     gene            2754..3119
                     /locus_tag="VFF22_13010"
     CDS             2754..3119
                     /locus_tag="VFF22_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17766.1"
                     /translation="MSVSISRQHWQALLGELEGAQRQRHLLTYRALIERLALPSPAMQ
                     TLAAALEHLAALDARAERPLRSALVISQGASRLPRTGFFECVSRLGRFSGASDGIAAA
                     SWHAAEVARVFEFSYPEDA"
     gene            3119..3589
                     /locus_tag="VFF22_13015"
     CDS             3119..3589
                     /locus_tag="VFF22_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014852166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HZX17767.1"
                     /translation="MFGALRARIGYLLARRLFHWAWLVKQPRAWRWLEGQFARMANLG
                     DTQAQSFYGHILLFRGQGLGARNEGRRLLGLAAKAGDAKAAYQLGVLSLSGDTQHAAN
                     AQQAIDWWTLAAQAGHPAAAYRLAELYRSGAAGVAADAAQAEHFAALSNRLSGL"
     gene            complement(3612..5372)
                     /locus_tag="VFF22_13020"
     CDS             complement(3612..5372)
                     /locus_tag="VFF22_13020"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HZX17768.1"
                     /translation="MIVTEQFSALDSILANGDLHSLFQPIFSLSERRILGYEALSRGP
                     SNSALHSPINLFAVARSAGRLSELELLCRKNACQRFSQFKLEGKLFLNVSPDSLMEPK
                     HQPGRTLQLLQSLGIPPSQVVIELTEQSPTDDFALLDTALHHYRAMGFSIALDDLGAG
                     YSSLRLWSELRPDYVKIDRHFIDGIHQDAVKREFVGSILKMAKASRAQVIAEGIELPE
                     ELAVLAEMGVDLIQGYLLCRPQEQPPREANNLLPSTTSSSPLSEDVSDLRGLLNPQRA
                     VALSTPTSEVLEMFRAQANLNSLAVLNEAQQPAGIVHRHSLAEALLKPFANELFSRKP
                     ISRLMSDDYLAVESSQSLQQVSRLLTSRARQRIEEDFIIIEDGCYVGLGRVIDVLKLI
                     TELKIQQARYANPLTLLPGNVPIQQCLTRLLQQSREAVVCYVDIDNFKPFNDIYGYAK
                     GDEVLLSLAQCLNDRVDPTRDFVGHIGGDDFLLVLGSQDWRARLNRLLEDFQSQCRRF
                     YREEDLHAGCFIAHDRNGKRQEYALLSLSLGVVHVHPEQCAQLDASQLAALASEAKRH
                     AKAVPGYSLHIIDTKADTAA"
     gene            complement(5466..7547)
                     /locus_tag="VFF22_13025"
     CDS             complement(5466..7547)
                     /locus_tag="VFF22_13025"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HZX17769.1"
                     /translation="MKRSLLSVTLAFSFALSALPLAAKTTSDDSWEYLQPDREQVIAS
                     LNVVELLKRHHYNKPPLNDERSEKIYQGYLKMLDPSRSYFIASDIAEFDQWRTRFDDL
                     LKNGDLQPGFAIYTRHLERLQSRLQFALDMLENGVDTIDFDSDESLLIDRENAPWVKD
                     LAELDDLWRKRVKDEVLRLKIAGKEPKAIQELLTKRYNNQLARLKQTSGEDVFQAYIN
                     AFAMSYDPHTTYLSPDNAENFDINMSLSLEGIGAVLQSDNEHVKVVRLVPAGPAEKSK
                     QIAPADKIIGVAQGSKEMVDVIGWRLDEVVKLIRGPKGSQVRLEVIPASNAPNDQTSK
                     VVAITREAVKLEEQAAKKSILNLKHDNRDYKLGVIEIPAFYLDFKAFRAGDPNYKSTT
                     RDVKRLLTELEKEKVDGVVIDLRNNGGGSLQEATELTGLFIDQGPTVLVRNSDGRVDV
                     LADENTGIYYKGPLAVLVNRLSASASEIFAGAMQDYHRALILGGQTFGKGTVQTIQPL
                     NHGELKLTLAKFYRVSGQSTQHQGVIPDIRYPDIMDTKEIGESALPEAMPWDSIKPAI
                     KPELDPIKPFLVELQSRFEDRTAQNPDFTFARERLQLAQKLMEENTVSLNETKRRAQQ
                     TDIEGQQLAIENSRRKAKGEALLNKLKEEDEDALPVEDEKTKPEDDAYLAESGRILLD
                     YLGLNTRLAKQ"
     gene            7676..8641
                     /locus_tag="VFF22_13030"
     CDS             7676..8641
                     /locus_tag="VFF22_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HZX17770.1"
                     /translation="MKALQGVEGRVDWAEYPAPACDVGEIRIRVAAAGLNRADLLQRA
                     GLYPPPPGASEVLGLECAGVVSEVGAGCDWKIGDRVCALLSGGGMAEEVVVDARHALP
                     VPEGMTLVEAAALPEVYATAWLNLFQLAGLKPGQKVLLHAGASGVGSAAIQLCKAFGS
                     PCWVSVGSPERLAYCESLGAQGGVLRGEEQLQALRDFAPFDVILDPVGGSYAALNLDL
                     LAVDGRWVNIGLLGGRKAELDLAAVLGKRITLIGSTLRNRDEQFKADLLRDLQQFVWP
                     LFAEGRLKSQLQQAYALKDAEAAFAALASNQVNGKLVLVIDPTIV"
     gene            8757..9320
                     /gene="ahpC"
                     /locus_tag="VFF22_13035"
     CDS             8757..9320
                     /gene="ahpC"
                     /locus_tag="VFF22_13035"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HZX17771.1"
                     /translation="MNLINTQVQPFKANAFHNGKFIEVTEQSLKGQWSVLIFMPAAFT
                     FNCPTEIEDAANNYAAFSDAGTEVYIVTTDTHFSHKVWHETSPAVGKAQFPLVGDPTH
                     ALTRAFGVHIEEEGLALRGTFLINPEGVIKTVEIHSNEIARDVSETLRKLKAAQYTAA
                     HPGEVCPAKWKEGEKTLAPSLDLVGKI"
     gene            9473..11026
                     /gene="ahpF"
                     /locus_tag="VFF22_13040"
     CDS             9473..11026
                     /gene="ahpF"
                     /locus_tag="VFF22_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HZX17772.1"
                     /translation="MLDANLQTQLKAYLEKVTLPFEIVASLDDSAKSQELLELLQSIV
                     GLTDKITLKTDGSDARRPSFSLNRPGANIGLTFAGIPMGHEFTSLVLALLQVGGHPSK
                     LDAETIEQIKSIDGSFEFETYYSLSCQNCPDVVQALNLMAVLNPNIRNVSIDGALFQE
                     EVERRQVMAVPSIYLNGEVFASGRMDVKEILAKIDTGSANRDAEKMSAKQAFDVLVIG
                     GGPAGAAAAIYAARKGIRTAIAAERFGGQVLDTMAIENFISVKETEGPKLVRALEEHV
                     REYEVDIMNLQRASALVPASSEGGLHEVKFENGASLKAKTVILSTGARWREMGVPGEQ
                     EYKAKGVCFCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLLEFADTLRAD
                     AVLQKKLYSLPNVTVIKSAQTTEVKGDGQKVNALVYKDRTSEELHSVELEGIFVQIGL
                     LPNTDWLKGSVELSRFGEIIVDAKGATNIPGVFAAGDVTTVPYKQIVIAMGDGAKASL
                     SAFDHLIRS"
     gene            complement(11097..11675)
                     /locus_tag="VFF22_13045"
     CDS             complement(11097..11675)
                     /locus_tag="VFF22_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003112334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZX17773.1"
                     /translation="MRYSEDHKAQTHQRIIEEAARLFRRDGVGATGLQPLMKTLGLTH
                     GGFYAHFKSKDELVETALRHSAEKLTTATSEMANSDKPLTRLISGYLSSAHRANPGDG
                     CPLPTMSAELGQRGAPSPVTDELIRDRLQTIEAGLSVEHADEQSVLILSAMVGALLLS
                     RSVSDPELSDRLLKTTRRLLIEQNTNNVAQSA"
     gene            complement(11716..12297)
                     /locus_tag="VFF22_13050"
     CDS             complement(11716..12297)
                     /locus_tag="VFF22_13050"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003135314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZX17774.1"
                     /translation="MSIPSREEKLAEWLAAEQVMRASLGAPGSMSLAQVSELLPQEFF
                     DGIGSGELPSPPIGHLLDFVPLEWSSGRFVFQGTPDARHYNPLGSVHGGYAATLLDSC
                     MGCAIHTQLQRGQGYTTTDLRISYIRALNTKVGPIRAEGRIVHIGRTTALAEGRIYDV
                     DDRLYAVGSTTCLILSMPGAAPAPGTETPGRSG"
     gene            12418..13626
                     /locus_tag="VFF22_13055"
     CDS             12418..13626
                     /locus_tag="VFF22_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017139690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX17775.1"
                     /translation="MPPYPLPSRSLLLILVALTALGEISTQLIIPSLGVIELIMNARP
                     GSSLLALSAFVAAFGLGQLLLGPLSDRIGRRPVLVAGLLVYLLATSWMLMASNMEEFI
                     AARVLQGLGACTALVLARAIVRDVWQAQAGPALALTVIGMLCAIVLSPMLGGVLTLYG
                     GWRAPIVLALLIGVAIVCAVLGLYRESNRQLDPQAGQLSRLAGNYAELLRAASFRALA
                     LTLSCTYGAMFAVVAGSSAVYVGLLGLSPAQYGITFAAIVSALIAGALFTQRRILQLG
                     PEKIVGIGAALVASGALATLAIYLLFGLSLLGLSLPQVLVTLGGGMLLPASVAGAVIP
                     NAHRAGLAAGFMGFAQMAGATCSGVLLSLLADGSAWPMVLLNSLFAVAAFCAFHLLRP
                     RPAANAALVR"
     gene            13644..15044
                     /gene="lpdA"
                     /locus_tag="VFF22_13060"
     CDS             13644..15044
                     /gene="lpdA"
                     /locus_tag="VFF22_13060"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HZX17776.1"
                     /translation="MSNYDVLIIGGGPGGYNAAIRAGQLGLKVACIEGRETLGGTCLN
                     VGCMPSKALLHASELYEAATSGELNNLGIEVTPTLNLAQMMKQKDESVTALTKGIEFL
                     FRKNKVEWIKGWGRLNGPGKVTVTLADGSVRELQAKDIVIATGSEPTPLPGVTIDNQR
                     ILDSTGALALPKVPKHLVVIGAGVIGLELGSVWRRLGSEVTVVEYLDRICPGLDNETA
                     KTLQRTLSKQGINFKLATKVSAAKATAKGVTLDLQPAAGGAVEQLQADYVLVAIGRRP
                     YTAGLGLETVGLATDKRGMLENQQHRSSVSGIWIIGDVTSGPMLAHKAEDEAIACIER
                     IAGHAAEVNYEAIPGVIYTKPEVATVGKTEEQLKAEGRAYKVGKFPFTANSRAKINHE
                     AEGFVKVLADERTDQILGVHIIGPSAGELIGEYCVALEFSASAEDIALICHPHPTRSE
                     ALRQAAMNVHGWAMQA"
     gene            complement(15124..15963)
                     /locus_tag="VFF22_13065"
     CDS             complement(15124..15963)
                     /locus_tag="VFF22_13065"
                     /EC_number="3.5.3.26"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allantoicase/(S)-ureidoglycine
                     aminohydrolase"
                     /protein_id="HZX17777.1"
                     /translation="MSQTTYYAPHGGHPDQKQLLTDRAMFTEAYAVIPKGVMRDIVTS
                     HLPFWDNMRMWVIARPLTGFAETFSQYIVEVAPEGGSERPELDQNAEAVLFVVEGQID
                     ITLEGKHHTLVPGGYAFLAPGAEWSLRNNSKANVTFHWLRKHYQKVEGLPAPESFVTH
                     RDNATVIEMPGTEGAWKTTRFVDMADMRHDMHVNIVTFQPGGVIPFAETHVMEHGLYV
                     LEGKAVYRLNQDWVEVEAGDFMWLRAFCPQACYSGGPGPFSYLLYKDVNRHMPLPLTG
                     QPR"
     gene            16105..16968
                     /locus_tag="VFF22_13070"
     CDS             16105..16968
                     /locus_tag="VFF22_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZX17778.1"
                     /translation="MLDPLLLRAFLTVVQTGGFTRAAASLHLTQSTVSQQVRRLEEQV
                     GAQLLERGGRYVVTTAEGDRMLGYANRIVSLIDEAELAMGRNQQDGEVRLGVPDDFAA
                     DSLTSCLADFADAHPGIRLEIINGLSHDVWNAFTAGELDLALIKQRIGSVKGLASWPE
                     PLAWMDSRARPVLARNPAPLAVFPSNGLYRLEMTHALDAMGKPWRIAYVSSNLSGVSA
                     AAAGGLGLTLLPRRLLTAAHRELGAAEGFAPVADVEVALHARQQLPAHARELALALTG
                     ACERIMQSTEA"
     gene            complement(17048..18169)
                     /gene="zapE"
                     /locus_tag="VFF22_13075"
     CDS             complement(17048..18169)
                     /gene="zapE"
                     /locus_tag="VFF22_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017256431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HZX17779.1"
                     /translation="MDCSSPLTAYQQAIDRDGFKSDPAQWQAAQALHDCHQALHQTAP
                     AHPINGVYLWGPVGRGKTWLMDSFYKGLKVPARRQHFHHFMRWVHMRLFQLTGTPDPL
                     QALAVELSHEVRVLCFDELFVSDIGDAIILGRLFQVMFEQGVVLVATSNQPPELLYAE
                     GFNRERFLPAVAALQRHMQVVSINGEEDHRLHPGTAHTRYWVATPGLPSVLSRTFSQL
                     AAGRPSSDEPLSLGNRSVQTLRHCDGVLWCRFADLCEQPLAAPDFIALCDRFKAILVS
                     EVPNLSSPQRAAKIARGTEDGVERVQAGDRQLPALSVKDDSVRRFIALVDECYDRKVP
                     LYLEAQVPLTELYTEGYLSFAFRRTLSRLQEMQLQRFGS"
     gene            complement(18169..18603)
                     /gene="arsN2"
                     /locus_tag="VFF22_13080"
     CDS             complement(18169..18603)
                     /gene="arsN2"
                     /locus_tag="VFF22_13080"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HZX17780.1"
                     /translation="MIFYRHPPAEAAKALLATAGLPTADLAAEHFAHFIAAGPAHAPE
                     ALIGLQPYGDVALLRSLVVSPTTRGKGYGGALVAEIEAYAQQLGVRELYLLTNSTESF
                     FSRRGYSSVERASVPEVIRQTAEFSSLCPDSAVCMHKRIETR"
     gene            complement(18712..19365)
                     /locus_tag="VFF22_13085"
     CDS             complement(18712..19365)
                     /locus_tag="VFF22_13085"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZX17781.1"
                     /translation="MALAIFDLDETLIGGDCASLWSQEMVKLGWADGDSFIAREQELM
                     RQYSAGELAMEDYMDFTLSPLVGRTPEEVAHVVEPFVEDVIEPIFYSDATRWLANHRA
                     AGDRVLVISASAHFLVSAVAARLKIEEVLAINLEVQHGHYSGRTQGILTYREGKVSRL
                     QEWLQEQGETLDGAYFYSDSRNDLPLLQVVEKPYVVNPDPTLLQHAQQAGWPILSWR"
     gene            complement(19365..20354)
                     /locus_tag="VFF22_13090"
     CDS             complement(19365..20354)
                     /locus_tag="VFF22_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016983773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX17782.1"
                     /translation="MSYLSIQGLHKSYGDTAIFSDINCEIAKGEFITLLGPSGCGKST
                     LLRCIAGLTGVNGGQILLDGEDLVPVAPQKRGIGMVFQSYALFPNMTVQQNVAFGLRM
                     QKVKGSEAEQRVKEVLELVELNDFAGRYPQQLSGGQCQRVALARSLVTRPRLLLLDEP
                     LSALDARIRKHLREQIRSIQQELGLTTIFVTHDQEEALVMSDRIFLMNAGKIVQSGDA
                     ETLYTAPADAFAAGFIGNYNLLDADAASRLLLRPVNSRVAIRPEAIQIGAQGSIEGQI
                     RSHRLLGNVIRYRVEARGVELLVDVLNRSPADLHASGQRINLNIEDNAICEVA"
     gene            complement(20351..21145)
                     /locus_tag="VFF22_13095"
     CDS             complement(20351..21145)
                     /locus_tag="VFF22_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZX17783.1"
                     /translation="MSHAEKRSPLFHQLVVYSLLLILLLPLLGTLLYSFSTSWSASVL
                     PSGLTFKWYLALWSDARFLAAFGRSLLVCLAALALSLVLILPLLFVVSYHFPKLDAVM
                     NVLILLPFAIPPVVSSVGLMQLFAGGPLPILGTPWILIGCYFTIALPFMYRAISNNLQ
                     AINLRDLMDAAHLLGASTWRAAFMVVLPNLRKGLMVSVFLSFSFLFGEFVFANLLVGS
                     RYETLQVYLYNMRNDSGHFTSALVISYFMFVLLMTWAANRLNKDQS"
     gene            complement(21132..21971)
                     /locus_tag="VFF22_13100"
     CDS             complement(21132..21971)
                     /locus_tag="VFF22_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZX17784.1"
                     /translation="MSALRGKWLALLCLLPFALFFIAFQIAPLLWVASNSLHTTEGWG
                     LGNFQQAFASPFYRQAMRHSLEVAFWSSLIGISIAILGSYSLRQVDSKLRDFVMAFSN
                     MTSNFSGVPLAFAFIILLGFNGALTILLKQAGIIEDFNLYSKTGLIVLYTYFQIPLGV
                     LLLYPAFDALREDWRESAELLGASNWQFWRHIGLPVLTPALLGTFVILLANALGAYAT
                     VYALTTGNFNILPIRIAAMVSGDIFLDPNMASALAMILVGLMTLITIVHQWLLRRSYH
                     VPR"
     gene            complement(21971..22777)
                     /locus_tag="VFF22_13105"
     CDS             complement(21971..22777)
                     /locus_tag="VFF22_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZX17785.1"
                     /translation="MSHNVILVVLDGLSYQVAQHALGHLQAYCQAGRAALYKLDCELP
                     ALSRPLYEAILTGVAPIDSGIVNNDVTRLSTQRSMFHYARDAGLITAAAAYHWVSELY
                     NRSPFQAARDRHTSDANLPIQYGHFYYADHYPDSHLFADAESLRLRHAPNLLLVHPMN
                     IDDAGHKHGLDSAQYRNSARMADVLLAEYIQPWLDAGYQILVTADHGMNNDRSHNGLL
                     PEEREVPLLVLGSAFSLNPNAQPKQLELCGTVCELLGVAHDKPVCRELLA"
BASE COUNT         4295 a         6434 c         7369 g         4728 t
ORIGIN      
        1 attagcccta gggcgcaccg gacaaggaac aagcatgaaa cagcatcgtt tagcagcggc
       61 ggtcgccctg gtaggtttgg ttctcgcagg ttgcgattcg caaaccagcg aaatcaccct
      121 ggaaacccca gcacagaaag cctcctacgg tatcggcctg aacatgggca aaagcctggc
      181 tcaggaaggc atggatgacc tggattccga cgccgttgct caaggcatcg aagacgccat
      241 tggcaagaaa gaacagcgcc tgaaagacga agagctgatc gaagcctttg ccttcctgca
      301 aaaacgtgcc gaagagcgca tggttactct gaacgaagag tcggccaaag ccggcaagaa
      361 gttcctggaa gacaacgcca agcgtgaagg cgtgaccacc accgcttccg gcctgcagta
      421 cgaagttgtg aagaaggctg acggcgctca gccgaaagcc actgacgtgg tgactgttca
      481 ctacgaaggc aagctgaccg atggcagcgt gttcgacagc tcggttgagc gtggtagccc
      541 gattgatctg ccggttaacg gcgtgatccc aggttgggtt gaaggtctgc agctgatgca
      601 cgttggcgag aagtacaaac tgtacatccc aagcgagctg gcgtacggcg agcagagccc
      661 gactccggcg attccagcca attcggtgct ggtgtttgat ctggagctga ttgcgatcaa
      721 agacgcagcc actcaagagt aatttctagc gttttccgca gcgccccgtc tcgacggggc
      781 gttgtcgttt ctacgagtca caatcggcac tttccgcttg actggagagt cttaatgggt
      841 gtgtgtagtg gctgtggtgt tacggttatg cacatttcaa aaattggccg tgacgggtta
      901 gggtaactct ctttcagaaa ccctttgcca tttttaactt attgatttat atggattttt
      961 actgtgtggg caaaaaatgt tcgatctgtg gtgaggccgc atgggctggg cgtttgccag
     1021 ggttatcgag aaactgttca caaggttatc cacagcttta gtggataacc atagccagcc
     1081 cagcgggcac gcgggattgc caggagaggg cgatgaaaac accgaaaaat ttcgcgcgtt
     1141 accagttttt agccaagcgt ttcctcaagg ccgggcggtt acctgcattg ctgttatcgg
     1201 tcgctcgaaa gcgcgaaaaa ctcggtaatg gctttgccga attggccgag caactgaagt
     1261 tactgcaagc gctttgcttg gcgtggtggc gcggtgaata tcgagcaatc gattcccgag
     1321 cgctgctggc ggtggtggcg gcgttgctgt acttcgttac gccgctggac gccataccgg
     1381 actggttgtt tggcattggt ctggttgatg atctggctgt gctggcctgg gtgctgcgta
     1441 cctggcagga agagctggcg gtgtttcagc agtggcgcgc ggctcaggct ccggaggtgc
     1501 tgcgggtcgt tgagcggctg ccagagttgg atgaatcgct agcgccgttg cacgggcggg
     1561 tggagtagtg ggcgacgatt cagatgtcgt tgagcatctg ctcgattagg ccctggtcgc
     1621 gtaattgctg cagtgttctg tccaatagct cggcgctgac gggcaattgc gggctgtagg
     1681 tacagtgcat gtcgtagcgt tcgattaccc aggggtttat ctgcagtggt gcatctgcac
     1741 gtttggtcag ctggaagcgc agcggccggc gcatgtcgat gatggcgtcc aggcgttgtt
     1801 tgcgcagcaa gttaagcccg gcgctgtggt cgcgcagatc ctggcggatg atctgcttac
     1861 tggcgaatgc cgccatcaac tcactgctgt agacgtaacc cagctgagtg ccgatggttt
     1921 tgccatagag ttcgtgcagg ctgttgatcg gcgcatgctg gcggtgcaat agcagcacgt
     1981 cctcttctct gtagagcggg gcggaccagt gatagcgctc ggggttgtct atccattgtg
     2041 ggttgctgtt acagattaca tggatgcgac cgcgctgcat aaattcatcg atgcgcttgt
     2101 tcggggtttc gacaaacgca gcctgataac ccagttgctg gctggccagc tggcccagct
     2161 tgtagataac gccgccgcgc aattgctgct cgtgcacatc gacatagggc atgccgtcag
     2221 ccgtggcgaa gccccagcgc agctcatctg cgcaggccca gccgctgcag ccaatcaaca
     2281 gcattgttat cagtgtgcgc aaagcctgcc ctcaactgcc gcttgataac ctgtgggcag
     2341 tatagatcaa gctctttatc gtgccgggct gttaagatgg tcgcctgttc tggagttgct
     2401 gagagtgatg gaagtgctct gcatggagat ttacagatga atgtgcaagt gatcgagcgc
     2461 gatggtcagc cggagtatgc ggtgttgcct tgggcgcaat atcaggcatt gctgcaggcc
     2521 gccgggcgcg cgcctgaggt ggctgctgtg gcagtggatg ccagcaagcc ggccctgagc
     2581 cagctggcga ccctacgcga agccaagggt atgagtgccg aacagctggc gcgcaatgtc
     2641 ggtatcagcc cgcattacct gagcatgatc gagagcggcg aacgccagcc ggacgccgcc
     2701 atccagcgcg cgctggcctg gcaccttggg gtcgatgggt gggaggcgca gccttgagcg
     2761 taagcatcag tcggcaacat tggcaggcgt tgctcggtga gcttgaaggt gcccagcgtc
     2821 agcgtcacct actgacctat cgcgcgttga tcgagcgtct ggccttgcct agccctgcca
     2881 tgcaaacctt ggcagccgcg ctggagcatc tggcggcact ggatgcacgc gccgagcgcc
     2941 cgctacgcag tgcgctggtg atcagccagg gtgccagccg tttaccacgt acaggtttct
     3001 ttgagtgtgt tagccgattg gggcgcttta gcggtgcgtc cgatggtatc gccgctgcgt
     3061 cctggcatgc cgccgaagtg gcccgcgtat tcgaattcag ctatccggaa gacgcctgat
     3121 gtttggtgcc ctgcgcgcac gtatcggcta cctgctggcg cgtcgtctgt ttcactgggc
     3181 gtggctggtc aagcaaccac gtgcctggcg ctggctggaa gggcagttcg cccgcatggc
     3241 caatctgggt gatacccagg cgcaaagctt ctacggacat atcctgctgt ttcgggggca
     3301 gggactgggc gcacgcaacg aaggtcggcg tttgctagga ttggcggcca aggccggtga
     3361 tgccaaggct gcttaccagc tcggtgttct gagtctttcc ggcgataccc aacacgcggc
     3421 gaatgcgcag caggccatcg actggtggac cctggccgca caggccggcc acccggctgc
     3481 agcctatcgc ttggctgagt tgtaccgcag cggcgctgca ggtgttgctg ccgatgcagc
     3541 ccaggctgag cattttgccg cgctttccaa ccgcctcagc ggcctctgat tcagtcgctc
     3601 ggcctctgcc atcaggccgc cgtatcagcc ttggtgtcga tgatatgcag gctgtaacct
     3661 ggcactgcct tggcgtgacg cttggcctct gaggccaggg ctgccagttg gctggcatcc
     3721 agttgcgcgc actgctcggg gtgcacatgc accacgccca gtgaaagtga gagcagcgcg
     3781 tattcctggc gtttgccgtt gcggtcatgg gcgataaagc agcccgcgtg caggtcttct
     3841 tcacgataga aacgccgaca ctggctctgg aagtcttcca gcaggcgatt gagccgcgcc
     3901 cgccagtcct gcgagccgag caccagcaga aagtcatcgc caccgatatg gccgacaaaa
     3961 tcccgcgttg ggtcgacccg gtcgttcagg cactgcgcca ggctgagcag cacttcatcg
     4021 cctttggcgt agccgtagat gtcgttgaac ggcttaaagt tatcaatatc cacataacac
     4081 accacggctt cgcggctctg ctgcaacagg cgggtcaggc attgctggat cggcacattg
     4141 cccggcagca gggtcagcgg gttggcgtag cgggcctgct ggatcttcag ctcggtgatt
     4201 agcttgagca cgtcaatcac ccggccgagc ccgacataac agccgtcttc aatgatgatg
     4261 aaatcttctt caatacgctg gcgggcgcgg ctagtaagca gacggctgac ctgctgcagc
     4321 gactggctgg actccaccgc caggtagtca tcactcatca ggcggctgat gggtttgcgg
     4381 gagaacagtt cgttggcaaa cggcttgagc agggcttcgg ccagcgagtg gcgatggacg
     4441 atgccagcgg gttgctgcgc ctcgttgagt accgccagcg agttcaggtt ggcctgggca
     4501 cggaacatct ccagcacttc acttgttggg gtgctcaggg ccactgcacg ttgtgggttg
     4561 agcagaccgc gcaggtcgct gacgtcctcg ctcagcgggc tgctagaggt ggtgctgggc
     4621 agcagattgt tggcctcacg cggcggttgc tcttgcgggc ggcagagcag gtagccctgg
     4681 atcaggtcga cgcccatttc cgcgagtacg gcgagttctt caggcagttc gataccttcg
     4741 gcgattacct gggcgcggga ggctttggcc attttcagta tggagccgac gaactcgcgt
     4801 ttgaccgcgt cctgatgaat gccgtcgata aagtggcggt cgatcttcac ataatccgga
     4861 cgcagttctg accacaggcg caggctcgaa tagcccgcgc caaggtcatc cagtgcgatg
     4921 gaaaagccca tggcgcggta gtggtgcagg gcagtgtcga gcagagcaaa gtcatcggta
     4981 ggcgattgct cggtcagctc gatcaccacc tgactcggtg gaatgcccag gctttgcagt
     5041 agctgcaggg tgcgccccgg ttgatgcttg ggctccatca gcgagtccgg cgagacgttg
     5101 aggaacagct tgccttcaag cttgaattga ctgaaacgct ggcaggcatt cttgcggcac
     5161 agcagctcca gctcgcttag gcggcccgcg ctgcgcgcca cagcaaacag gttgatcggc
     5221 gagtgcaggg cgctgttgga tgggccacgg ctgagggctt cgtagccgag aatgcgccgc
     5281 tcggagagcg agaaaattgg ttggaacagg ctgtgcaagt cgccattggc gaggatgctg
     5341 tccaacgcgc tgaactgctc ggtgacgatc atggcggctc tctgtgcata aaaaacgggg
     5401 actggcattg ctaccagtcc ccgcatttca cgacagtttg atgacccttt gatgacgctc
     5461 gctgtttact gtttggccag gcgcgtgttc aagccgaggt agtcgagcag gatgcgcccg
     5521 ctttcggcga ggtaggcatc atcttccggt ttggtttttt catcttccac cggcagggcg
     5581 tcttcgtctt cttccttgag cttgttcagc agcgcttcac ctttggcttt gcggcggctg
     5641 ttctcgatgg ccaactgctg gccttcgata tccgtctgct gggcgcggcg cttggtttca
     5701 ttgaggctga cggtgttttc ttccatcagc ttctgcgcca gttgcaggcg ttcacgggcg
     5761 aaggtgaagt ccgggttttg cgcggtgcgg tcttcaaaac ggctctgcag ctcgacgagg
     5821 aacggcttga tagggtccag ctccggcttg atcgctggct tgatgctgtc ccacggcata
     5881 gcctcgggca gggcgctctc accgatttcc ttggtatcca tgatgtctgg gtagcggatg
     5941 tcgggaatca cgccctggtg ctgggtgctt tgcccagaga cgcggtagaa cttagccagg
     6001 gtcagcttca gctcgccatg gttgagcggc tggatggttt gcacggtgcc tttgccgaag
     6061 gtctggccgc cgagaatcag cgcgcggtga tagtcctgca tagcgccggc gaaaatttcc
     6121 gaagccgagg ccgacaggcg attgactagc acggccagtg gccctttgta gtagatgccg
     6181 gtgttttcat cggccagtac atcaacccgg ccgtcgctgt tacgcaccag tacggtcggg
     6241 ccctggtcga taaacaaacc ggtcagctcg gttgcttcct gcagcgagcc gccaccattg
     6301 ttgcgcaggt cgatgactac gccgtcgact ttttcctttt ccagctcggt aagcaggcgt
     6361 ttgacgtcac gggtggtgct tttgtagttc ggatcgccag cacggaaagc cttgaagtcc
     6421 aggtaaaagg ctggaatctc gatcacccca agcttgtagt cacggttatc gtgcttaagg
     6481 ttgaggatgg atttcttcgc ggcttgctct tccagcttga ccgcttcacg ggtgatggcg
     6541 accaccttgc tggtctgatc gttcggtgca ttgctcgctg gaatcacttc caggcgcact
     6601 tgcgagcctt tcggtccgcg aatcagcttg actacttcat ccaggcgcca gccgatcacg
     6661 tcgaccattt ctttgctgcc ttgtgccacg ccaatgattt tgtcggcagg ggcgatctgc
     6721 ttgctctttt ccgccggacc ggccggcacc agtcgcacga ccttgacgtg ttcgttgtcg
     6781 ctttgcagca cagcaccaat accttcgagt gacaaactca tattgatgtc gaagttttcc
     6841 gcgttgtccg gcgacaggta ggtggtgtgc gggtcgtagg acatggcaaa ggcgttgata
     6901 taggcctgga acacgtcttc gccgctggtc tgcttgagcc gtgcgagctg gttgttgtag
     6961 cgcttagtca gcaactcctg aatcgctttg ggttctttgc cggcgatttt caggcgcagc
     7021 acttcatctt tgacgcgttt gcgccagagg tcgtcgagtt cggcgagatc cttgacccag
     7081 ggcgcgttct cgcggtcgat cagcaggctt tcgtcgctgt cgaaatcaat tgtgtctacg
     7141 ccgttttcga gcatgtccag ggcgaactgc aggcggctct gcaagcgctc caggtggcgc
     7201 gtgtagatgg caaagccggg ttgcagatcg ccgtttttca gcagatcgtc aaagcgtgtg
     7261 cgccattgat cgaactcggc gatatcagag gcgataaagt agctgcgcga agggtcgagc
     7321 atcttcaggt agccctgata gatcttctcc gaacgctcgt cattcagcgg tggtttgttg
     7381 tagtgatggc gcttcaacaa ctccacaacg ttgaggctgg cgatgacctg ttcgcggtca
     7441 ggctgcaggt attcccagga gtcgtcactg gtggttttcg ccgcgagcgg taaggcgctc
     7501 agggcgaatg aaaaggcgag ggtgacgctg agtagggatc gcttcatgct aattcgacgt
     7561 gtgggcaatt gataacgcat attaggccgt ctttaagggc gccaggttcc atcggcgcaa
     7621 tgcaaaagcc cagcggtcag cgccgggctc ggttcaaatg cactatggag gcagcgtgaa
     7681 ggcattgcaa ggcgttgaag gtcgtgtgga ttgggcagaa tacccagcgc cggcctgtga
     7741 tgtaggggaa attcgcatcc gtgtagcggc cgctgggtta aatcgtgccg acttgttaca
     7801 gcgcgccggg ctttatccgc caccgcctgg cgccagtgag gtgcttgggc tggagtgcgc
     7861 cggtgtggtc agcgaggtag gcgccggttg tgactggaaa atcggtgacc gtgtttgcgc
     7921 gctattgagc ggtggcggca tggccgaaga ggtggtggtc gatgcgcgcc acgccctgcc
     7981 ggtcccggag ggtatgacgc tggttgaggc ggcggcactg cccgaggtgt acgccacggc
     8041 ctggctcaat ctgtttcagc tggcgggttt aaagcctggg cagaaggtcc tgttgcatgc
     8101 cggcgccagt ggtgttggtt cggcagctat tcaattgtgt aaagccttcg gcagtccgtg
     8161 ctgggtcagc gtcggttcgc cggagcgctt ggcctactgc gaaagcttgg gcgctcaggg
     8221 cggggtactg cgcggtgaag aacagctgca ggcgctgcgc gatttcgccc cgttcgatgt
     8281 gatcctcgat ccggtgggcg gcagctacgc cgcacttaat ctggatttgc tggctgtgga
     8341 tggccgttgg gtaaatatcg gtttgctcgg cggacgcaag gccgagctgg atctggcggc
     8401 agtgttgggc aagcgcatta cgttgatcgg ctcgaccctg cgcaatcgtg atgagcagtt
     8461 caaggccgat ttattgcgcg atttgcagca gtttgtctgg ccgcttttcg ccgagggccg
     8521 actcaaatcg caacttcagc aggcttatgc gctcaaagat gctgaggccg cctttgctgc
     8581 attggcgagt aatcaggtca acggcaagtt ggtgctggta atagacccga caatagtttg
     8641 aagccggtag tcgattggct taatcaatcg gaatcatggc ggcaatcaat aagctttttt
     8701 tgctcaaggc gttaagatgc gccctgtaaa tttctaaccc tcaaaaggaa ccagagatga
     8761 atctcatcaa cacccaagtt caaccgttta aggctaacgc tttccacaac ggcaaattca
     8821 tcgaagtgac cgaacagtcc ctgaaaggcc agtggtcggt actgatcttc atgcccgctg
     8881 ctttcacctt caactgcccg actgagattg aagacgctgc taacaactac gcagcgttta
     8941 gcgatgcagg caccgaagtt tacatcgtca ccaccgacac ccatttctcc cacaaagtct
     9001 ggcacgaaac ttcgccagct gtaggcaagg ctcagttccc gctggtgggt gatccgaccc
     9061 acgcactgac ccgcgctttc ggtgtgcaca tcgaagaaga aggtctggct ctgcgcggca
     9121 ccttcctgat caacccggaa ggcgtgatca agaccgttga aatccactcc aacgaaatcg
     9181 ctcgtgacgt atcggaaact ctgcgcaagc tgaaagctgc tcagtacacc gctgctcacc
     9241 caggcgaagt gtgcccagcc aagtggaaag aaggcgagaa gactctggct ccgtcgctgg
     9301 acctggttgg caagatctaa atctgccaac accttccctg gccctaggct cggggcaggg
     9361 agtacttagc gctgtcaggg tcgcaacccg accctagtgg tgcccgatgc ccgggcgcga
     9421 tccgctcggg cattttattg cccagaattt ttgtcattga ggaccatccg ccatgttgga
     9481 cgctaacctg caaacccagt tgaaggccta cctggaaaag gtcaccctgc cgttcgagat
     9541 cgtcgcgtcc ctcgatgaca gtgcgaaatc ccaggagctg cttgaactgc tccaaagcat
     9601 cgtcggccta accgacaaga tcaccctgaa gaccgatggc agcgacgccc gtcgtccgtc
     9661 gttctcgctc aaccgcccag gcgccaacat cggcctgacc ttcgccggca ttcccatggg
     9721 ccacgagttc acctcgctgg tactggcgtt gctgcaggtg ggcggccatc cgtccaagct
     9781 ggatgccgag accatcgagc agatcaagag catcgacggt tcgttcgagt tcgagaccta
     9841 ttactcgctg tcctgccaga actgcccgga cgtggtccag gcgctgaacc tgatggctgt
     9901 gctcaacccg aacattcgta acgtctccat cgacggcgcg ctgttccagg aggaagtcga
     9961 gcgccgtcag gtgatggccg tgccgagcat ctacttgaac ggtgaagtgt tcgcttccgg
    10021 ccgtatggac gtgaaggaaa tcctcgccaa gatcgacacc ggctccgcca accgcgatgc
    10081 cgagaagatg agtgccaagc aagccttcga cgtactggtt atcggtggcg gcccggccgg
    10141 tgctgcagct gctatttatg ccgcgcgtaa aggtattcgt accgcgattg ccgccgagcg
    10201 tttcggcggt caggtgctgg acaccatggc catcgagaac tttatctcgg tcaaggaaac
    10261 ggaagggccg aagttggtgc gtgcgctgga agaacacgtg cgtgagtacg aagtcgacat
    10321 catgaacctg cagcgtgcca gcgcactggt gccggcgagc agcgaaggcg gcttgcatga
    10381 ggtgaagttt gagaatggtg cgagcctcaa ggccaagacc gtaatcctct ctaccggtgc
    10441 gcgctggcgc gagatgggtg tgccgggcga gcaggaatac aaggccaagg gcgtgtgctt
    10501 ctgcccgcac tgcgacggcc cgctgttcaa gggtaagcgc gtggcggtga tcggcggcgg
    10561 taactccggc gtcgaggcgg cgattgacct cgccggtatc gttgcccacg taaccctgct
    10621 ggagtttgct gacaccctgc gtgccgatgc cgtgctgcag aagaagcttt acagcctgcc
    10681 gaacgtcacc gtgatcaaga gtgcgcagac caccgaggtt aaaggtgatg gtcagaaggt
    10741 caatgcgctg gtctacaagg accgcaccag cgaagagctg cacagcgtcg agctggaagg
    10801 catcttcgtg cagatcggcc tgctgcctaa caccgactgg ctgaagggta gcgtagagct
    10861 gtcgcgtttc ggggaaatca tcgtcgacgc caagggcgcc accaatatcc cgggtgtatt
    10921 tgccgccggt gacgtaacta ccgtgccgta caagcagatc gtcattgcca tgggcgatgg
    10981 tgccaaggcc tccttgagcg ccttcgatca tctgatccgc agctaacaag ctagaagctc
    11041 tacgtcgcac aaagcggaca cctttcaccg gttgtccgct tttttttgcg ctgcggtcag
    11101 gcgctttgcg ccacgttgtt ggtgttctgt tcgatcagca ggcggcgggt tgttttcagc
    11161 aagcggtcag acagttctgg gtcgctgacg ctgcgtgaca gcagcagggc accgaccatg
    11221 gcgctgagga tcagcacgct ttgctcatcg gcatgctcaa cgctaaggcc ggcttcgatg
    11281 gtttgcaggc ggtcgcggat cagttcatcg gtcaccgggc tgggcgcgcc gcgttggccc
    11341 agctcggcgg acatggtcgg cagcgggcag ccatcgcccg gattggcgcg gtgggcggac
    11401 gacaggtagc cgctgatcaa gcgggtcagc ggtttatcgc tgttggccat ctcgctggta
    11461 gcggtcgtca gcttttcagc actgtggcgc agggcggttt ccaccagctc atctttcgat
    11521 ttgaagtggg cgtagaagcc gccatgggtc agccccaggg ttttcatcag cggctgcagg
    11581 ccggtggcac cgacgccgtc gcggcgaaac aggcgcgcag cctcttcgat gatgcgttga
    11641 tgggtctggg ctttgtggtc ttcggaatag cgcatggagt acctcgccgc tggtttttag
    11701 atgttgcctg ccatcttacc cgcttcgccc cggtgtttca gttccgggtg ccggcgctgc
    11761 cccaggcatg ctgagaatca ggcaggtggt tgagccgacg gcgtacaggc ggtcgtctac
    11821 atcgtaaatg cgcccttcgg ccagcgcggt ggtgcggccg atatgcacga tgcggccttc
    11881 ggcgcggatc ggcccgactt tggtgttgag ggcgcgaatg tagctgatgc gcagatcggt
    11941 ggtggtgtaa ccctggccgc gctgcaattg ggtgtggatg gcgcagccca tgcaggaatc
    12001 gagcagggtg gccgcataac cgccgtgcac gctgcccagc gggttgtagt gacgcgcatc
    12061 aggcgtgccc tggaagacga agcgcccgga agaccactcc agcggaacaa aatccagcaa
    12121 gtggccgatg ggcggcgagg gcagttcacc gctgccgatg ccgtcaaaga actcctgcgg
    12181 caatagttcg ctgacctgcg ccaggctcat gctgccgggt gcaccaaggc tggcgcgcat
    12241 gacctgttcg gcggcgagcc attcggccag tttttcttcg cgtgatggga tgctcatgga
    12301 tcaggcctcg tcggttagtg gctttaaaat tacaagcgta atttaattat ttatattatg
    12361 gttgaaatat tagtgttcgg cgagccacgc cagcccgtga ttacaggacc cacgcccatg
    12421 cctccctatc ccttgccgtc gcgcagcctg ctactgattc tggtggccct gaccgccctt
    12481 ggagagattt ccacccagct gatcatcccc agcctgggcg ttatcgaact gatcatgaat
    12541 gcgcgccccg gcagcagcct attggcttta tcggcctttg ttgccgcttt tggcttgggc
    12601 cagttgttgc tcggcccgct gtcggatcgt atcggacggc gtccggtgct ggtcgccgga
    12661 ctgttggttt atctgctcgc cacctcatgg atgctgatgg ccagcaacat ggaagaattt
    12721 attgccgccc gagtactgca agggcttggt gcttgcactg cattggtatt ggcgcgggcc
    12781 atcgtccgcg atgtgtggca ggcgcaggct gggccggcat tggcgctgac ggtgatcggc
    12841 atgctttgcg ccattgtcct gtcgccgatg ctgggcggcg tgctgacgct gtatggcggt
    12901 tggcgcgcgc caattgtttt ggcactgctg atcggggttg ccatcgtttg cgcggtgttg
    12961 ggcttgtacc gcgaaagcaa tcggcaactc gatccccaag ccgggcagtt gagccgcttg
    13021 gccggtaact atgccgaact gttgcgcgcc gcctcgttcc gcgcgctggc cctgacgctg
    13081 tcttgcacct atggcgcgat gtttgccgta gttgccggct cttcggctgt gtatgtcggc
    13141 ctgctcgggc tgagcccggc gcaatacggc attaccttcg cggcgattgt ttcggcgcta
    13201 atcgccggcg cattgttcac ccagcgacgc attctgcagc ttggccccga gaagatagtc
    13261 ggcataggcg cggcactggt ggcgagcggt gccctggcca ccttggcgat ttatctgctg
    13321 tttggtttgt cattgctcgg cctgtcgtta ccccaggtgt tggtcaccct gggcggcggt
    13381 atgttgctgc cggcatcggt ggccggcgcg gtgatcccca atgcccaccg cgccggcctg
    13441 gccgcaggct ttatgggctt tgcgcaaatg gccggtgcca cctgcagcgg tgtcttgctt
    13501 agcctgctgg cggatggctc ggcttggcct atggtgttgc tgaacagttt attcgccgtc
    13561 gcggcgttct gcgccttcca cctgctgcgc ccacggccgg cagcgaacgc ggccctggtt
    13621 cgctaaaccc taggagcaat tccatgagca actatgacgt gctgatcatc ggtggcggcc
    13681 ccggcggcta caacgcggcc atccgtgccg ggcaactggg cctcaaggtc gcctgcatcg
    13741 agggtcgcga gaccttgggc ggcacatgcc tgaatgtcgg ctgcatgccg tcgaaggcgc
    13801 tgctgcatgc ctcggagctg tacgaagcgg cgaccagcgg cgagctgaat aacctcggta
    13861 tcgaggtcac gccgacgctc aatctggcgc agatgatgaa gcagaaggat gagagcgtaa
    13921 cggcgctgac caagggcatc gagtttctgt ttcgcaagaa caaggtcgaa tggatcaagg
    13981 gctggggccg cttgaacggg ccgggcaagg ttacggtgac cctcgccgac ggcagtgtgc
    14041 gcgagttgca ggccaaggac atcgtaattg ctaccggctc ggagccgacg ccgttgcctg
    14101 gggtgacgat tgataaccaa cgcatcctcg actccaccgg cgcgctggct ttgcccaagg
    14161 tgcccaagca cctggtggtg atcggtgccg gggtaatcgg tctggagctg ggttcggtgt
    14221 ggcggcgttt gggcagcgaa gtgacggtgg tggagtacct cgaccgcatt tgcccaggcc
    14281 tggataacga aaccgcgaaa accctgcagc gcaccctgag caagcagggc ataaatttca
    14341 aactggcaac caaggtcagc gccgccaagg ccacggcaaa aggtgtaacc cttgatctgc
    14401 agccagctgc tggcggtgct gtcgagcaac tacaggctga ttatgtgctg gtcgccattg
    14461 gtcgccgccc ttataccgcc ggcctggggc tggaaaccgt cggcctggcc accgacaagc
    14521 gcggcatgct ggaaaaccag cagcaccgca gcagcgtttc gggcatctgg atcatcggcg
    14581 atgtcacctc cgggccgatg ctcgcgcaca aggccgaaga tgaagccatt gcctgtatcg
    14641 aacggattgc cgggcatgct gccgaggtca attacgaggc gattcctggc gtcatctaca
    14701 ccaaaccgga agtcgccacc gtgggcaaga ccgaggagca gctgaaggcc gaagggcgcg
    14761 cctacaaggt cggcaagttt ccctttaccg ccaacagccg ggcgaagatc aatcacgagg
    14821 ccgaaggctt cgtcaaagtg ctggccgatg agcgcactga ccagattctc ggcgtgcata
    14881 tcatcggccc cagcgccggc gagttgatcg gtgagtactg cgtggccctg gagttcagcg
    14941 cctcggctga agatattgcg ctgatctgcc atccccatcc gacgcgctcg gaagcgttac
    15001 gtcaggcggc gatgaatgtg catggctggg ccatgcaggc ctgaaactag tcacaatctg
    15061 cggtgcgttg gcctgctgcg ccgcaagcag gctcgcgaag ctgcttgcgg ctgacgtgct
    15121 gctttagcgc ggctggccgg tcaggggcag cggcatatgg cggtttacgt ccttgtacag
    15181 caggtagctg aacgggcctg ggccgccgga gtagcaggct tgcgggcaga atgcgcgcag
    15241 ccacatgaag tcgccggctt caacctcgac ccagtcctgg ttcaggcggt acaccgcctt
    15301 gccttcgagc acgtacaggc cgtgttccat cacgtgggtc tcggcgaacg ggatcacgcc
    15361 gccgggctgg aaggtcacga tgttgacgtg catgtcatgg cgcatgtcgg ccatgtcgac
    15421 gaagcgggtg gtcttccaag cgccttcggt gcccggcatt tcgatgacgg tggcgttgtc
    15481 gcggtgggtg acgaacgact ccggcgccgg cagcccttcg actttctggt aatgcttgcg
    15541 cagccagtgg aaggtgacgt tggccttgct gttgttgcgt aggctccact cggcacctgg
    15601 cgcgaggaag gcgtagccgc ccggtaccag ggtgtggtgc ttgccttcga gggtgatgtc
    15661 gatttggcct tcgaccacga acaggaccgc ttcggcgttc tggtccagct cggggcgctc
    15721 gctaccgcct tcaggggcga cttcaacgat gtattgcgag aaggtttcag caaaccccgt
    15781 cagcgggcgg gcaataaccc acatgcgcat gttgtcccaa aacggtagat ggctggtgac
    15841 gatgtcacgc atcacgcctt tggggataac ggcataggct tcggtgaaca tggcgcgatc
    15901 agtcagaagc tgtttctgat caggatggcc gccatgcggg gcgtagtaag ttgtttggct
    15961 catcgagaag ggcctcagtg aaatgtcggc aatagatcga cgggaaagcg taatcccggc
    16021 gtggtttcac tataggaagt tgtgcttgga ttctgaaatt aaatagtaga atccgaggca
    16081 gtggctttgt gaataggttg cctcatgctt gatccgctgt tgttacgcgc ttttctaact
    16141 gtggtgcaga cgggcgggtt tacccgggca gccgcgagcc tgcacttgac ccagtcgacg
    16201 gtcagccagc aagtccgtcg actggaagag caggtaggcg cgcagttgct cgagcgcggt
    16261 ggccgctacg tagtgactac cgctgagggt gaccgcatgc tcggttatgc caaccgtatc
    16321 gtctcgttga tcgatgaggc ggagctggcc atggggcgca atcagcagga cggcgaggtg
    16381 cgcctaggcg tgccggacga cttcgccgcc gatagcctga cttcctgtct ggcagacttt
    16441 gctgatgccc atccgggtat tcgcctggaa atcatcaatg ggctcagcca tgacgtgtgg
    16501 aacgccttca ctgccggcga gctcgatctg gctttgatca agcagcgtat cggcagcgtc
    16561 aaggggctgg ccagttggcc ggagccactg gcctggatgg acagtcgcgc acggccagtg
    16621 ctggcgcgta atccggcgcc gctggcggtg tttccatcta acggcctgta ccgcctggaa
    16681 atgacccacg cgctggacgc catgggtaaa ccctggcgta tagcctatgt cagcagcaac
    16741 ctgtccggag tcagtgctgc tgctgcaggt gggctggggc tgacgttact gccacgccgg
    16801 ctgcttaccg cggcacaccg cgagttgggt gccgccgaag gctttgcgcc ggtggccgat
    16861 gtggaagtag cgctgcatgc ccggcagcag ctcccggccc atgcccgcga gcttgcgctg
    16921 gcgttaacgg gcgcttgcga gcgaatcatg cagtcaactg aggcctgagt tagcgccagt
    16981 tgaggattgg tcagccggcc tgctgcgcgt gctgccaccg tgggcaagtc cgaggagcgg
    17041 tacaggctta agaaccgaag cgctgcaact gcatctcttg caagcggctg agtgtgcggc
    17101 gaaaggcgaa gctcaggtag ccctcggtat agagttcggt cagcggcacc tgggcttcca
    17161 gatacagcgg caccttgcgg tcgtagcact catccaccag agcaataaag cgccgcacgc
    17221 tgtcatcctt gactgacagg gcgggcagct ggcgatcacc ggcctgcacc cgctcgacgc
    17281 catcttcggt gccgcgggcg attttcgccg cgcgttgcgg gctgctcagg ttgggcactt
    17341 cgctaaccag aatggccttg aagcggtcgc acagggcgat aaagtccggg gcggccaaag
    17401 gttgttcgca cagatcggca aaacggcacc agagcacgcc gtcgcagtgg cgcagggtct
    17461 gcacgctacg gttgccgagg ctaaggggct cgtcactgct gggccggcct gcggccaact
    17521 gactgaaggt ccggctgagc acgctgggca gccctggcgt ggcgacccag tagcgggtgt
    17581 gggcggtgcc tgggtgtagg cgatggtctt cctcaccgtt gatgctgacc acctgcatat
    17641 gccgctgcaa ggcggccacc gcaggcagaa agcgctcgcg gttaaagcct tcggcataca
    17701 gcagttcggg cggctggttg gaggtggcga ccagcaccac gccctgttcg aacatcacct
    17761 gaaacaggcg gccgagaatg atcgcatcgc cgatatcgct gacaaacagc tcatcgaagc
    17821 acagcacgcg cacctcatgg ctcagctcca cggccagcgc ctgcagcggg tccggcgtgc
    17881 cagtgagctg aaacaggcgc atatgcaccc agcgcataaa gtggtgaaag tgctgacgcc
    17941 gcgccgggac tttcaggcct ttgtagaaac tgtccatcag ccaggtcttg ccacggccca
    18001 ccggccccca cagatacacc ccattgatcg gatgtgcggg tgcggtctgg tgcaaggcct
    18061 gatgacaatc gtgtaacgcc tgggcggcct gccactgcgc cgggtcggat ttaaaaccgt
    18121 cgcggtcgat ggcttgctgg taggcggtga gcggggagct gcagtccatc agcgcgtctc
    18181 gatccgcttg tgcatacaca ccgcactgtc ggggcagagg ctggagaact cggctgtttg
    18241 gcgaatcact tcaggcacgc tggctcgttc gacgctgcta taaccacgtc ggctgaagaa
    18301 gctttcggtg ctgttggtca gcaggtacag ctcacgtacg ccgagttgct gcgcataggc
    18361 ttctatctcg gcgaccagcg cgccgccata acccttgccg cgcgtcgttg ggctgaccac
    18421 cagtgagcgc agcagcgcca cgtcgccata aggctgcaag ccgatcagcg cttcgggtgc
    18481 gtgcgctggg ccagcagcaa taaagtgcgc gaagtgttcg gcggcaaggt cggccgttgg
    18541 cagtccggca gtggccagca acgccttggc ggcctcagcc ggcgggtgac ggtaaaaaat
    18601 catggacgtc catctgggcg agataatcgg atcagcacag cgctggcact ttacccgagc
    18661 gcatagtaga agacccatag gtgtgggtct tttacattca accgagcgct atcagcgcca
    18721 gctcaggatc ggccagccgg cctgctgggc gtgctgcaac agggtcgggt cggggttgac
    18781 cacatagggt ttttcgacca cctgcagcag cggcaggtcg ttgcgcgaat cggagtagaa
    18841 gtaggcgccg tcgagggttt cgccttgttc ttgcagccat tcctgcagac ggctgacctt
    18901 gccttcgcgg taggtcaata tgccctgggt acggccgctg tagtggccgt gttggacttc
    18961 gagattgatg gccagtactt cttcgatctt caagcgcgcg gcgacggcgc tgaccaggaa
    19021 atgcgccgag gcggaaatca ccagcacccg gtcaccggcg gcgcggtggt tggccagcca
    19081 gcgggtggcg tcgctgtaga aaataggttc gatcacgtct tcgacaaagg gctcgaccac
    19141 gtgggcgact tcttccggtg tgcggcccac caggggcgac agggtgaagt ccatatagtc
    19201 ttccatggcc agctcgccag cgctgtattg gcgcatcagc tcctgctcgc gggcgataaa
    19261 gctgtcgcca tcggcccagc cgagtttgac catttcctgg ctccacaggc tggcgcagtc
    19321 gccgccgatc agggtttcgt ccaggtcgaa aattgccagg gccatcaggc cacctcacag
    19381 attgcgttgt cttcaatgtt caggttgatg cgctgcccgc tggcgtgcag gtctgccggg
    19441 gagcggttga gcacgtccac cagcagttcg acgccgcgcg cctcgacgcg gtaacggatc
    19501 acgttgccga gcagacgatg gctgcggatc tgcccttcga tactgccctg ggcgccaatc
    19561 tggatggcct ccgggcggat tgccacgcgg ctgttgaccg ggcgcagcag caggcggctg
    19621 gcggcatcgg catcgagcag gttgtagttg ccgataaagc cggcggcaaa ggcatcggcc
    19681 ggggcggtgt agagagtttc cgcgtcgccg ctctggacga tcttgccggc gttcatcagg
    19741 aagatacggt cgctcatgac cagcgcttct tcctgatcat gggtgacgaa tatggtggtc
    19801 aagcccagct cctgctggat gctgcggatc tgttcgcgca ggtgcttgcg aatccgcgca
    19861 tccagcgccg acaacggctc gtcgagcagc agcaggcgcg ggcgggtgac cagtgagcgg
    19921 gccagggcca cgcgctggca ctggccgccg gacagttgct gcgggtagcg gccagcgaag
    19981 tcattcagct caaccagctc cagcacttcc ttgacccgct gctcggcttc gctgcctttg
    20041 accttctgca tgcgcaagcc aaaggcgacg ttttgctgca cggtcatgtt ggggaacagc
    20101 gcgtagctct ggaacaccat gccgatgcca cgtttttgcg gagcgacggg taccagatcc
    20161 tcgccgtcga gcagaatctg cccgccattg actccagtca ggccagcgat gcaacgcagc
    20221 agggtggatt tgccgcagcc ggacgggccg agcagggtga tgaattcgcc cttggcgatt
    20281 tcgcagttga tatcgctgaa gatcgcggta tcaccgtagc tcttgtgcag gccttgaata
    20341 ctcagataac tcatgactgg tccttattca ggcggttggc cgcccaggtc atcagcagca
    20401 cgaacatgaa gtaggaaatc accagggcgc tggtgaagtg accgctgtcg ttgcgcatgt
    20461 tgtagaggta cacctgcagg gtttcgtagc ggctgcccac cagcaggttg gcgaagacga
    20521 actcgccgaa caggaagctg aaggagagga acaccgagac catcaggccc ttgcgcaggt
    20581 tgggcagcac caccataaag gccgcgcgcc aggtgctggc gccgagcagg tgggcagcgt
    20641 ccatcaggtc gcgcaggttt atcgcctgca ggttgttgct gattgcccgg tacataaacg
    20701 gcagggcgat ggtgaaatag cagccaatca gaatccacgg cgtaccgagg atcggcagcg
    20761 ggccgccggc gaacagctgc atcagaccga ccgaggacac caccggcggg atggcgaacg
    20821 gcagcaggat cagcacgttc atcaccgcat caagtttggg gaagtgataa ctgaccacaa
    20881 acagcagcgg cagaatcagc accagcgaca gcgcaagcgc cgccaggcag accagcagcg
    20941 agcggccaaa cgcggcgagg aagcgtgcat cgctccacag cgccaagtac cacttgaagg
    21001 tcagcccact gggcagcacg ctggccgacc agctggtgga aaacgagtag agcagggtgc
    21061 cgagcaacgg cagcaaaagg atcagcagca ggctgtagac caccagctgg tggaacagcg
    21121 gcgagcgttt ttcagcgtgg gacatggtag ctccggcgca gcagccattg gtggacgatg
    21181 gtgatcaggg tcatcaggcc aaccagaatc atcgccaggg cactggccat attcgggtcg
    21241 aggaagatgt cgccggagac catggcggca atccgaatcg gcaggatgtt gaagttacct
    21301 gtggtcagtg catacaccgt ggcgtaggcg ccgagggcgt ttgccagcag aatcacgaag
    21361 gtaccgagca gcgctggggt cagcacgggc aagccgatat gccgccagaa ctgccagttg
    21421 ctagcgccca ataattcagc ggattcgcgc cagtcttcac gcagggcgtc gaaggccggg
    21481 tagagcagca gcacaccgag tggaatctgg aagtaggtgt agagcacgat caggccggtt
    21541 ttcgagtaga ggttgaaatc ctcgatgatc cccgcctgct tgagcaggat ggtcagcgcg
    21601 ccgttgaacc ccagcaggat gataaaggcg aaggccagcg gcaccccgga gaagttgctg
    21661 gtcatattgg aaaacgccat aacgaagtcg cgcaatttgc tgtcgacttg ccgcagcgag
    21721 tagctgccga ggatggcgat gctgatgccg atcaggctcg accagaacgc cacctccagg
    21781 ctgtgacgca tggcctgacg gtagaacggc gaggcgaagg cctgctggaa attgcccagc
    21841 ccccagcctt cagtggtgtg caggctgtta ctggccaccc agagcagcgg ggcgatctga
    21901 aaggcgataa aaaacagggc aaaaggtagc aggcacaaca gcgccagcca tttgccgcgc
    21961 agggcagaca tcatgcgaga agctcccggc agacaggttt gtcgtgggcc acgccgagca
    22021 gttcgcagac cgtgccgcat aactccagct gtttcggctg ggcgttgggg ttgaggctga
    22081 aggcgctgcc gagtaccagc agcggcactt cgcgctcttc cggcagcagg ccgttgtggc
    22141 tgcggtcgtt gttcatgccg tggtcggcgg tcaccagaat ctggtagccg gcatcgagcc
    22201 agggctggat atattcggcc aatagcacat cggccatgcg cgcgctgttg cggtactgcg
    22261 cgctgtcgag gccgtgcttg tggccggcgt cgtcgatatt catcgggtgc accagcagca
    22321 ggttgggcgc atggcgcagg cgcaggcttt ccgcatcggc gaacaggtgc gaatccgggt
    22381 agtggtcggc gtagtagaag tggccgtact gaatcggcag gttggcgtct gacgtatgac
    22441 ggtcgcgcgc agcctggaat ggcgagcggt tatacaactc gctgacccag tgataggccg
    22501 ccgcagcggt aatcagcccg gcgtcgcggg cgtagtggaa catgctgcgc tgggtcgata
    22561 ggcgcgttac atcgttattg acgatgccgc tgtcgatggg cgcaacaccg gtcagaatcg
    22621 cttcatacag cgggcgtgac agggccggga gttcacagtc gagcttatac agcgcggcgc
    22681 gtcccgcctg gcagtaagcc tgcagatgcc ccagcgcgtg ctgagccacc tgatagctca
    22741 agccatccag aaccacgagg atgacgttgt gcgacatgat tgaaagcctt agcaaccgaa
    22801 tcggccccgg agtgtatccg gggcca
//
LOCUS       DASRUM010000200        11012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1772364, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000200 DASRUM010000000
VERSION     DASRUM010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 11012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11012
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1772364"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..1921
                     /gene="speA"
                     /locus_tag="VFF22_13110"
     CDS             8..1921
                     /gene="speA"
                     /locus_tag="VFF22_13110"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012270333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HZX17786.1"
                     /translation="MSARRTRKDDGSQWTVADSRSVYGIRHWGAGYFAINDKGRVEVR
                     PNGPQSTPIDLYEQINELRSSGLSLPLLVRFPDILQDRVRQLTGAFDANIARLEYQSP
                     YTALYPIKVNQQEAVVENIIATQNVSIGLEAGSKPELMAVLALAPKGGTIVCNGYKDR
                     EFIKLALMGQKLGHNVFIVIEKESEVQLVIDEAAELKVAPQIGLRVRLSSLASSKWAD
                     TGGEKSKFGLSAAQLLSVVERFRAAKLDQGIRLLHFHMGSQIANLSDYQHGFKEAIRY
                     YGELRGLGLPVDHIDVGGGLGVDYDGTHSRNASSINYDMDDYAGVVVGMLKEFCDAQE
                     LPHPHIFSESGRSLTAHHAMLVVQVTDVEKHNDEIPKIEDVTSLPETVRYLVELLGPT
                     DIEMVTETYWRATHYMSDVATQYADGKLSLTEKALAEQCYFAVCRRLHNSLKARQRSH
                     RQVLDELNDKLADKYICNFSVFQSLPDTWAIGQVLPILPLNRLDEEPLRRAVLQDLTC
                     DSDGKIRQYVDEQSIETSLPVHEVREGEDYLLGIFLVGAYQEILGDMHNLFGDTDSVN
                     IYQNADGSVYHAGIETHDTIEDMLRYVHLSPEELMNHYRDKVASAKISPRERTQYLDA
                     LRLGLTRSSYLSS"
     gene            complement(2056..3411)
                     /locus_tag="VFF22_13115"
     CDS             complement(2056..3411)
                     /locus_tag="VFF22_13115"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HZX17787.1"
                     /translation="MSDRYLAFANSTSGRRLVSALGLPAPLMLERWMSGRSRPVDGAL
                     LIGGEGSLASTVLPFANKLTDALFAPSEGQLELPRWTAEHGPKLKALVFDASGLTRFD
                     QLIELRDFFQPAFKGLDKCPRVVILGRAPESLKDPIAASVQRSLEGFTRSLGKEIRRG
                     GNVQLIYVGKGGEAQLEGALRFFLSPKSAYVSGQVLRLNACSEQVKDWTRPLAGKKAL
                     VTGASRGIGAAIAETLARDGAEVVLLDVPPAKDALDALAARLGGRGLALDICAADAAT
                     QLVEALPDGIDIVVHNAGITRDKTLAKMSDDFWNSVINVNLNAPQVLTQALLDAGKLH
                     DNGRVVLIASISGIAGNMGQTNYAVSKAGVIGLAQAWAPMLAKRGISINAVAPGFIET
                     QMTAAIPLTIREAGRRMNSMGQGGQPQDVAEAVAWFAQPGSGAATAQVMRVCGQSLLG
                     A"
     gene            3582..4859
                     /locus_tag="VFF22_13120"
     CDS             3582..4859
                     /locus_tag="VFF22_13120"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZX17788.1"
                     /translation="MTQPRRVAIVGGNRIPFARSNTVYATASNQEMLISALDGLVERF
                     NLHGERLGEVVAGAVLKHSRDFNLTRECVLGSRLAPETPAYDLQQACGTGLEAALLVA
                     NKIALGQIECGIAGGVDTTSDAPIGVNEGLRKILLQANRGKSTGEKIKSLLQIRPRHL
                     APAIPRNGEPRTGLSMGEHCELMAQTWAIPRDEQDQLAVASHQKLAASYAEGWHDDLL
                     TPFRGLARDQNLRADISLEKLATLKPCFERSPRGTMTAANSTPLTDGASLVLLASEDW
                     AKARGLPILAYWKDGEAAAVDFVGGKEGLLMAPAYAVPRMLARNKLSLQDFDYYEIHE
                     AFAAQVLSTLKAWEDADYCKTRLGLDAPLGSIDRSKMNVKGSSLAAGHPFAATGGRIV
                     ANLAKLLSVAQEGRGLISICAAGGQGVTAILEK"
     gene            complement(4962..6002)
                     /gene="ada"
                     /locus_tag="VFF22_13125"
     CDS             complement(4962..6002)
                     /gene="ada"
                     /locus_tag="VFF22_13125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HZX17789.1"
                     /translation="MLDPDSCWQAVCSRDSQCDGQFVFAVHSTGIYCRPSCPARRPLR
                     RNVSFQPTPAEAEQAGFRPCRRCSPQGLSPAEQLDQLVSVACRLLDGSDKPLTLAQLA
                     ARIGLSASHLARAFKTRTGLTPKAWSEAQRRARLEQQLPAADSVLEAALSAGYSGTRA
                     LYQQPATLTPAQRRKQGAGEQLRYCIAPCPLGHLLLASSAKGICALLFGDDPATLLTE
                     LQQRFAAAELHADDKGLGDSLRQVLAQLSEPQRAAQLPLDIRGTVFQQQVWRALQQIP
                     QGQTRNYAELAAQLGSHPRAIARACTSNPLGLLVPCHRVIASNGSLSGYRWGLARKAA
                     LLKQEAGPLLDQ"
     gene            complement(6091..6495)
                     /locus_tag="VFF22_13130"
     CDS             complement(6091..6495)
                     /locus_tag="VFF22_13130"
                     /inference="COORDINATES: protein motif:HMM:NF021496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2218 domain-containing protein"
                     /protein_id="HZX17790.1"
                     /translation="MRRAQSETSSMPQNSALYPRHALDSLAAQRHAHPERCNGINAPC
                     IASSYVATTTPARYISRLCTQFAHKLPVSFDEHYGRIEFAFGLSHLHADRTGLHITAL
                     SHNPQDLEKLKHLIAAHFERFAWQAELNLDWQ"
     gene            6845..7732
                     /locus_tag="VFF22_13135"
     CDS             6845..7732
                     /locus_tag="VFF22_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HZX17791.1"
                     /translation="MKTPKRIEPLVEAGLVDEVIRPLMSGKEAAVYVVRCGEELRVAK
                     VYKEANKRSFRQAAEYQEGRKVRNSRDARAMAKGSKYGRKGQEDAWQNAEVAALFRLA
                     SAGVRVPKPYDFLEGVLLMELVDDGEGDAAPRLNDVDLHPDDAREFHAFMIHEVVKML
                     CAGLVHGDLSEFNVLLDPGGPVIIDLPQAVDAAGNNHAFKMLERDVGNMSAYFGQFAP
                     ELKFTKYAKEMWALYEAGELTPESPLTGEFDDPEEEADIDAVMREIKAALADEARRQA
                     LLNAEDEPKGEEPPPPWAQ"
     gene            complement(7821..8690)
                     /locus_tag="VFF22_13140"
     CDS             complement(7821..8690)
                     /locus_tag="VFF22_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HZX17792.1"
                     /translation="MRWKRGRRSDNVVDARGSGSRMGGGKGLTLGGVAIVVIVGLLSG
                     QDPMQILGQLAGQAMQGGSVSAPQSSAPATNDEHSEFVRAVLGDTEDTWREIFQGANR
                     QYKDPTLVLFRGGVNSACGFANSAVGPFYCPGDQRVYIDLAFFRELEQRFGAAGDFAQ
                     AYVIAHEVGHHVQTLLGVSAKVNAARQRGERVEGATGLLVRQELQADCLAGVWAFHGQ
                     RRHDWLEPGDIEEALGAANAIGDDRLQKQARGQVVPDSFTHGTSEQRVRWFKTGFDGG
                     DVGRCDTFKATRL"
     gene            complement(8763..9203)
                     /locus_tag="VFF22_13145"
     CDS             complement(8763..9203)
                     /locus_tag="VFF22_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HZX17793.1"
                     /translation="MRQLLVLAGLFVSSLSFAAEPLTIDVHRDANCGCCKAWISHLQD
                     NGFNVVDHIETDMSEVKQRLGVPPRLSSCHTAVINGQFVEGHVPAADILKMRKQPDLL
                     GAAVPGMPAGSPGMEYGNVRDAYQVIGLDKQGAERVISEYPTIE"
     gene            9347..9733
                     /locus_tag="VFF22_13150"
     CDS             9347..9733
                     /locus_tag="VFF22_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 domain-containing protein"
                     /protein_id="HZX17794.1"
                     /translation="MLFARVVLVIQLLALAGLGLAYFVQPHEMTNLSGMLLMSPAAAT
                     DVRAFYGGLQLGLAAFLGLSLSRLDFTRAALTLLVLLYSALALARIGGLWLDGGAQQT
                     FNLYALLLEVVSVGLCFWALRGLQRV"
     gene            complement(9735..10226)
                     /locus_tag="VFF22_13155"
     CDS             complement(9735..10226)
                     /locus_tag="VFF22_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003152001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HZX17795.1"
                     /translation="MRRYLLALLPLLSACTSTPEPSIQVLQPTPGTSYQSFSVRAVRA
                     SGHRMDLEQRFNQAVQNALSAKGYRFTETQPDMQVIYALGLEQENGIELTPIAVGGTV
                     YTQTRATEDERARLALRILDSKTQAVLFQAQINRQLHNPDMSQENFDRGVAKILEGFP
                     PAR"
     gene            complement(10356..>11012)
                     /gene="trmA"
                     /locus_tag="VFF22_13160"
     CDS             complement(10356..>11012)
                     /gene="trmA"
                     /locus_tag="VFF22_13160"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HZX17796.1"
                     /translation="QVSIIGRSKGKRIVIGRDYVEEKLQVAGRTFSYRQPEGAFTQPN
                     GEVNQKMLSWAYDALGERSDDLLELYCGNGNFTLPLATRVRKVLATEISKSSVNAALA
                     NLADNGVDNVSLVRLSAEELTEALNEVRPFRRLAGIDLKSYDFGCVFVDPPRAGMDPD
                     TCELTRRFERILYISCNPETLAANIAQLSDTHRVTRCALFDQFPYTHHMESGVLLERC
                     "
BASE COUNT         2001 a         3314 c         3586 g         2111 t
ORIGIN      
        1 gacatcgatg tctgcacgac gcacacgtaa agatgacggc agccagtgga ccgtagcgga
       61 cagccgcagc gtctatggta ttcgccattg gggcgcgggt tacttcgcga tcaatgacaa
      121 gggccgtgtc gaggtgcgcc cgaatggtcc gcaaagcacg ccgatcgacc tctacgagca
      181 gattaatgag ctgcgctcca gcggcctgtc gctgccgttg ctggtgcgct ttccggacat
      241 tctgcaagac cgcgtgcgcc agctgaccgg cgccttcgac gcgaatatcg cgcggctgga
      301 ataccagagc ccgtacaccg cgctgtaccc gatcaaggtc aaccagcagg aagcggtggt
      361 ggaaaatatc atcgccacgc agaacgtttc catcggcctg gaagccggct ccaagccgga
      421 gctgatggcc gtgctggcgc tggcgccgaa gggcggcacc attgtctgca acggttacaa
      481 ggaccgcgaa tttatcaagc tggccctcat gggacagaag ctcggccaca acgtatttat
      541 cgtgatcgag aaagaatccg aagtgcagtt ggtgatcgac gaggcagccg aactcaaggt
      601 cgcgccgcag atcggtctgc gcgtacgcct gtcgtcgctg gcctcgagca agtgggctga
      661 cacaggcggc gagaagtcca agttcggtct gtcggcggcg cagctgctgt cggtggtcga
      721 gcgcttccgc gcggccaagc tggaccaggg catccgcctg ctgcacttcc atatgggctc
      781 gcagatcgcc aacctgtccg actatcagca cggcttcaag gaagccatcc gctattacgg
      841 tgagctgcgt ggcctgggcc tgccggttga ccatatcgac gtcggcggcg gcctgggagt
      901 ggattacgac ggcactcact cgcgcaacgc cagctcgatc aactacgaca tggacgacta
      961 cgccggtgtc gtggtcggca tgctcaaaga gttctgcgat gcccaggagc tgccacaccc
     1021 gcacatcttc tcggaaagcg gccgctcgct gactgcgcac cacgccatgc tggtggtgca
     1081 ggtcactgat gtggaaaaac acaacgacga gatccccaag atcgaagacg tcaccagcct
     1141 gccggaaacc gtgcgttacc tggttgagct gcttggcccg accgatatcg agatggtcac
     1201 cgagacttac tggcgcgcca ctcactatat gagcgacgtg gccactcagt acgccgacgg
     1261 caaactcagc ctgaccgaga aggccctggc cgagcagtgc tacttcgccg tgtgccgtcg
     1321 cctgcataac tcgctgaagg cccgtcaacg ctcgcaccgt caggtgctgg atgagctgaa
     1381 cgacaagctc gccgacaagt acatctgcaa cttctcggtg ttccagagcc tgccggacac
     1441 ctgggccatc ggccaggtac tgccgattct gccgctcaat cgtctcgacg aggagccgct
     1501 gcgccgcgcc gtgctgcaag acctgacctg cgactccgac ggcaagatcc gccagtacgt
     1561 cgacgagcag agcatcgaga ccagcctgcc ggtgcacgaa gtgcgcgagg gcgaggacta
     1621 cctgctgggg atcttcctgg tgggtgccta tcaggaaatt ctcggcgata tgcacaacct
     1681 gttcggcgac accgattcgg tgaacatcta ccagaacgcc gacggcagcg tgtaccacgc
     1741 cggtatcgag acccacgaca ccatcgagga catgctgcgc tacgtgcacc tgtcgccgga
     1801 ggagttgatg aaccactacc gcgacaaggt cgccagcgcc aagatcagcc cgcgtgagcg
     1861 tacccagtac ttggatgcct tgcgtttagg gctgacccgt tcgtcctatc tgtcgtcctg
     1921 atcgcgggca agcccgcgcc tacaaaagac aaggccccga attgcggggc cttttctttt
     1981 aaagcctggt attacgatct cgcgagccgg tttttaacgc agcagggccg acgcgcagca
     2041 gatcgtgcgc gtgcctcagg ccccgagaag gctctgcccg cagacacgca tcacctgcgc
     2101 ggtggccgcg ccggaacctg gctgggcgaa ccaggccacg gcttcggcaa cgtcctgggg
     2161 ttggccgccc tggcccatgg agttcatccg ccgaccggct tcgcggatgg tcagcgggat
     2221 ggcggcggtc atctgggttt cgataaaacc gggcgcaacg gcgttgatgc tgatgccgcg
     2281 cttggccagc attggtgccc aggcctgggc caggccgatc accccggcct tgctcactgc
     2341 gtagttggtc tggcccatat tgccggcgat gccgctgatc gaggcgatca gcaccacacg
     2401 gccgttgtcg tgcagcttgc cggcgtccag cagtgcttgg gtcagtacct gcggggcgtt
     2461 gaggttgacg ttgatcaccg agttccagaa gtcgtcgctc atcttcgcca gggtcttgtc
     2521 acgggtgatg ccggcgttgt gcacgacgat atcgataccg tccggcagcg cctcaacaag
     2581 ctgcgtggca gcatcggcag cgcagatatc cagggccagg ccgcgaccac ccaaacgtgc
     2641 ggccagggca tccaatgcat ccttggccgg cggcacgtcc agcagcacca cctcggcgcc
     2701 gtctcgggcc agggtttcgg cgatggccgc accgatgcca cgggaggcac cggtgaccag
     2761 ggccttcttg ccagccagcg ggcgggtcca gtccttgacc tgctcgctgc aggcattcag
     2821 gcgcagcacc tggccggata cataggcact cttcggcgag aggaagaagc gcagcgcgcc
     2881 ttccagctgc gcctcgccgc ccttgccgac atagatcagc tgcacgttgc cgccacggcg
     2941 gatttccttg cccagtgagc gggtgaaacc ttctagcgag cgctgcacgc tggcggcaat
     3001 cgggtctttc agcgactctg gagcgcggcc gaggatcacc acgcgcgggc atttatccag
     3061 gcctttgaag gccggctgga agaagtcgcg cagctcgatc aactgatcga aacgggtcag
     3121 cccgctggcg tcgaatacca gggccttgag tttggggccg tgttcggcgg tccagcgcgg
     3181 cagctcgagc tggccttcgc taggggcgaa cagggcgtcg gtgagcttgt tggcaaaggg
     3241 cagcacggtg ctggccaggc tgccttctcc gccaatcagc agggcaccgt ccaccggccg
     3301 gctgcgcccg gacatccagc gctccagcat tagcggcgcg ggcaggccca gcgcgcttac
     3361 cagacggcga cctgatgtgg agttggcaaa ggcgagataa cggtcggaca tggggcggct
     3421 cctgcgcatg gatcaaaagt gcgtccactc tacgcaggcc gcagaaatgc gcaattgacc
     3481 gcgccgcctg ctgtgccggc aaggcggcca atgccacact gtggcgagag gttagcctgt
     3541 gcgccattac tctcagaccc accccagatc aggagtcatc catgacccag ccgcgtcgcg
     3601 tcgccatcgt tggcggtaac cgcattccgt tcgcccgctc caataccgtc tacgccactg
     3661 cgagcaacca ggagatgctg atcagtgcgc tggatggcct ggtcgagcgc ttcaatctgc
     3721 atggtgagcg ccttggcgaa gtggtggccg gtgctgtgct caagcattca cgggatttca
     3781 acctgacccg tgagtgcgtg ctcggctcgc gcctggctcc ggaaacccca gcctatgacc
     3841 tgcagcaggc ctgcggcacg gggttggaag cagcactgct ggtggccaac aagatcgccc
     3901 tggggcagat cgaatgcggc atcgccggcg gcgtcgatac cacctcggac gcgccgattg
     3961 gggtgaacga agggctgcgc aagattctcc tgcaggccaa tcgcggcaag agcactggtg
     4021 agaagatcaa gagcctgctg cagattcgtc cgcgccatct ggctccggcg attccgcgta
     4081 atggcgagcc gcgcactggg ctgtcgatgg gcgagcattg cgaactgatg gcgcaaacct
     4141 gggcgatccc ccgtgacgag caggaccaac tggccgtagc cagccaccag aaacttgctg
     4201 cctcctatgc cgagggctgg catgacgatt tgcttacgcc gttccgtggc ctggcccgag
     4261 accagaacct gcgcgccgat atcagcctgg aaaaactcgc cactctcaaa ccctgcttcg
     4321 agcgcagccc gcgcggcact atgactgctg ccaactcgac gccactgacc gatggcgcat
     4381 ctctggtgct gctggccagc gaagactggg ccaaggcccg tggcctgccg atccttgcct
     4441 actggaagga cggcgaagcg gcggcggtgg atttcgttgg cggcaaggaa ggcctgctga
     4501 tggctccggc ctatgcggtg ccgcgcatgc tggcacgcaa caagctaagc ctgcaggatt
     4561 ttgactacta cgaaatacac gaagccttcg ccgcccaggt gctcagcacc ctcaaagcct
     4621 gggaagacgc cgactactgc aaaacccgcc tggggctgga tgcaccgctg ggttcaatcg
     4681 atcgcagcaa gatgaacgtc aagggcagct ccctggcggc cggccacccg tttgcagcca
     4741 ctggcgggcg catcgtcgcc aacctggcca agctgctctc ggtggcgcag gaaggccgtg
     4801 ggctgatctc gatttgtgcc gctggcggtc agggtgtgac tgcgatcctg gaaaagtaat
     4861 caaacagcgg tattcgcacg cggtgcgggt actgatgtat gcaactccgt gatttggagc
     4921 ggcccacgca ggtggtgccg ctcttttttt gttctgattt tttattgatc gagcagtggg
     4981 ccggcttcct gcttaagcag cgccgccttg cgtgccaggc cccagcggta gccgctgagg
     5041 ctgccattgc tggcgatcac ccggtggcac ggcactagca ggcccagcgg gttgctggtg
     5101 caggcgcggg ctatggcgcg tgggtggctg cccagttgcg cggccagttc tgcgtagttg
     5161 cgcgtctgcc cctgcggaat ctgctgcagg gcgcgccaca cctgctgctg aaacacggtg
     5221 ccacgaatat ccagcggcag ctgggcggcg cgctgcggtt cgctgagctg cgccagcacc
     5281 tgacgcaagc tgtcgcccag gcctttgtca tccgcgtgta gttcagcggc ggcaaagcgt
     5341 tgctgcagtt cagtcagtag cgttgccgga tcgtcgccaa acaacagtgc gcagatgccc
     5401 ttggcgctac tggccagcaa caagtggccg agggggcaag gcgcgatgca gtagcgcagt
     5461 tgttcgccgg cgccctgttt gcgccgttgt gccggggtca gggtcgctgg ctgctgatac
     5521 agcgcgcggg tgccggagta accggcgctt agcgcggcct ccagtacgct gtctgctgcg
     5581 ggcaactgtt gttccaggcg cgctcgacgt tgcgcctcgc tccaggcctt gggtgtcagt
     5641 ccggtgcggg tcttgaacgc gcgggccagg tgcgaggcgg acaggccgat acgggcggcc
     5701 agctgggcca gggtaagcgg cttgtcgctg ccgtcgagca ggcggcaggc gacgcttacc
     5761 agttggtcga gctgctcggc ggggctcagg ccctggggcg agcagcgccg gcaggggcga
     5821 aaaccggcct gttcggcctc ggccggggtg ggctggaaac tgacattgcg ccgtagcggc
     5881 cggcgcgccg ggcaactcgg ccggcagtag atgccggtgg agtgcacggc gaagacaaac
     5941 tggccgtcgc actggctatc gcggctgcag acggcttgcc agcagctatc ggggtcgagc
     6001 atcgcctggc tcccatgggg ctatgggccg attgtcgacc tttgccagcg ctacgccaat
     6061 ccgaatccga ctttcaaagg tcggcggccc tcattgccag tcgaggttta gctcggcctg
     6121 ccaggcaaag cgctcgaagt gcgcggcgat caggtgtttg agcttttcca ggtcttgcgg
     6181 gttatggctc agggcggtga tatgcaggcc ggttcggtcc gcatgcaggt gtgagagtcc
     6241 gaaggcgaac tcgatgcgcc cgtagtgttc atcaaaactg accggcagtt tgtgggcgaa
     6301 ctgcgtgcat agacggctga tatagcgcgc cggcgtggtg gtggcgacat agctggaagc
     6361 aatgcacggc gcgttgatgc cattacagcg ctcaggatgg gcatggcgct gggcggctag
     6421 gctgtccagt gcgtggcgtg ggtagagggc agagttctgg ggcatgctgc tggtctcaga
     6481 ttgggcacgt ctcatggtta tctcccccca atcactgcgg gcttaagcaa agtgcctgtg
     6541 ctgggtaaaa gtcaggtcca gtgcgttaaa gcatggagtg ctggccaaat atatagcttt
     6601 gggacagtgc ttaaagcctt ttggttgcat cgaacgcctg cttgtaacct ctaggttgct
     6661 tcgcctgggc gtttgaaacg tgccgctaac caccgtgttt gagcggtttt tgccaccttt
     6721 gcgaggcccg tgaaataccc cgcagtctgg gccgtgctga caggagggtg ggcgctgctt
     6781 aaagattgcc gtataacggg cggcagagtc gacttgggca acacaggacc ccagattaaa
     6841 gctgatgaag acgccaaaac gtattgagcc cctggtcgaa gccgggctgg tcgatgaagt
     6901 tatacgcccg ctgatgagcg gtaaagaagc cgcggtctat gtggtgcgct gtggcgaaga
     6961 actgcgagtc gccaaggtct ataaagaagc caacaaacgc agcttccgtc aggccgccga
     7021 gtaccaggaa ggccgtaagg tgcgtaacag ccgtgatgcc cgggccatgg ccaagggctc
     7081 gaagtatggt cgcaaaggtc aggaagacgc ctggcagaat gctgaagtgg cggcgctgtt
     7141 ccgcctggca agcgctgggg tgcgggtgcc caagccgtat gacttccttg aaggcgtatt
     7201 gctgatggaa ctggtcgatg atggcgaggg cgatgccgct ccacgcctca atgatgtcga
     7261 cttgcatccg gacgatgccc gcgaattcca cgcctttatg attcatgaag tggtgaaaat
     7321 gctttgcgcg ggtctggtgc atggcgacct gtcggaattc aacgtgctgc ttgatccagg
     7381 tggcccggtg atcatcgacc tgccacaggc ggtggacgct gccggcaaca accatgcgtt
     7441 caagatgctt gagcgtgatg tcggcaacat gtccgcctac tttggccagt tcgccccgga
     7501 attgaagttc accaagtacg ccaaggaaat gtgggcgctg tatgaggccg gtgaactgac
     7561 tccggaaagc ccgctgaccg gtgaattcga tgacccggaa gaagaggccg atatcgacgc
     7621 agtgatgcgc gagatcaagg ccgctctggc cgacgaagcg cgccgtcagg cgctgctgaa
     7681 tgccgaagac gaacccaagg gcgaagagcc gccaccgcct tgggcgcaat aggcacagag
     7741 tgctgctgat aaaaaatgcc gcttacctga tcaggtgagc ggcatttttt tgctctggat
     7801 atcttggatg tttgcgtcgc ttacagccgc gtggccttga aggtatcgca gcggccgaca
     7861 tcaccaccgt cgaaaccggt cttgaaccag cgcacgcgct gctccgaggt gccgtgggtg
     7921 aaagagtccg gtaccacctg gccgcgtgcc tgtttctgca agcggtcatc gccgatggcg
     7981 ttggcggcgc ccagtgcttc ctctatatcc cctggctcca gccaatcatg ccggcgttgg
     8041 ccgtggaagg cccacacgcc tgccaggcag tcggcctgca gttcctggcg caccagtagg
     8101 ccagtggcgc cttccacccg ctcgccgcgt tggcgggcag cgttgacctt ggctgagacg
     8161 ccgagcaggg tctgcacgtg gtggccgact tcatgggcaa ttacataggc ctgggcaaag
     8221 tcgccggcgg cgccaaagcg ctgttccaac tcgcggaaga acgccaggtc gatatacacc
     8281 cgctgatcgc ccgggcagta gaagggcccg accgccgaat tggcgaagcc acaggcggaa
     8341 ttgaccccac cgcgaaacag caccagggtc gggtccttgt actggcggtt ggcgccctgg
     8401 aagatttccc gccaggtgtc ctcggtatcg ccgagcaccg cgcggacgaa ttccgaatgc
     8461 tcgtcattgg tcgccggcgc agagctttgc ggcgcgctga ccgagccgcc ctgcatggct
     8521 tgcccggcca actggccgag gatctgcatc gggtcctggc cgctgagcag gccaacgatc
     8581 accacgatgg ccaccccacc gagggtcagc cccttgccac cgcccatgcg tgagccgctg
     8641 ccgcgtgcgt ccaccacgtt gtcactgcgt cttccgcgtt tccagcgcat tgctatcgtc
     8701 ctgtgtgtat gccgagcttg agtgtcgtcc gcaggcttga actacgccag tgcagtcgtc
     8761 acttattcaa tggtggggta ttcgctgatc acccgttctg cgccttgctt atccaggccg
     8821 atgacttgat aggcgtcgcg cacattgccg tattccatac ctggtgagcc tgcaggcatg
     8881 ccgggcacgg cagcgccgag caggtcgggc tgcttacgca ttttgaggat gtcggcggcg
     8941 gggacatggc cttctacaaa ctggccgttg atcaccgcgg tatggcagga cgacaggcgc
     9001 ggtggcacgc ccaggcgctg tttgacctca ctcatatcgg tttcgatatg atctaccacg
     9061 ttaaagccat tgtcctgcag gtggctgatc caggccttgc agcagccgca gttggcatcg
     9121 cggtgcacgt cgatggtcag gggttcggcg gcaaaactca ggctgctgac aaacagcccg
     9181 gcgagaacaa gcagctggcg catcgggtaa ctcctggcag gtgaaaggct gagcatagcg
     9241 ccgggcggcc gtggctaacc accaacgatt tccgaatcgg acgggcgcgg cttatgctgc
     9301 gtgctcgttt tatgggctct cgaattccaa gaaaaggatg ctgtccatgt tgtttgcacg
     9361 cgtagtactg gtgattcaac tgttggcatt ggcggggctg gggctggcct attttgttca
     9421 gcctcatgaa atgaccaatc tcagcggcat gctgctgatg tctccggccg ccgccactga
     9481 tgtgcgggcg ttctacggcg gcctgcaact gggcctggca gcgtttcttg gtttgtcgct
     9541 aagccggttg gacttcaccc gcgcagcgct gaccctgctg gtgctgctct acagcgcctt
     9601 ggccctggcg cgcatcggcg gcttgtggct ggatggcggc gcgcagcaga ccttcaatct
     9661 ctacgcgctg ctgctggaag tggtctcggt ggggctgtgc ttctgggcgc tgcgcggctt
     9721 gcagcgcgtc tgagtcagcg cgcgggtggg aagccttcaa ggatcttggc cacgccacgg
     9781 tcaaagtttt cctggctcat gtccgggttg tgcagctgcc ggttgatctg cgcctgaaac
     9841 agcacggcct gggtcttgct atcgagaata cgcagggcca ggcgtgcgcg ctcatcttcg
     9901 gtggcgcggg tctgggtgta gaccgtgccg cctacggcta tcggggtgag ctcaatgcca
     9961 ttttcctgtt ccaggcccag ggcataaatg acctgcatat caggctgcgt ttcggtgaag
    10021 cggtaaccct tagcgctgag tgcgttctgc accgcctgat taaagcgctg ctccagatcc
    10081 atacgatgac cactggctct gactgcacgc acgctgaagc tctgataaga ggtgccaggt
    10141 gtcggttgca gcacctgtat cgacggttcg ggcgtgctgg tgcaggcgct cagcaggggc
    10201 agcagggcta acagataacg acgcatcgac gactccttag cgggtggggc caggcaccct
    10261 ggtcagcgca gtttaggtct agagcgaaac cttgcgccaa tctgccgatg ggtctgctgt
    10321 ttgcgtcgcg tatcgctgtg ggtggcgcgg taacgttaac agcgttccag caatacaccc
    10381 gactccatat ggtgggtgta ggggaactgg tcgaacagcg cgcagcgcgt cactctgtgc
    10441 gtgtcgctga gctgggcgat gttggcggcc agggtttccg ggttgcagga gatatacaga
    10501 atgcgctcga agcgccgggt cagttcgcag gtgtctgggt ccatgccggc gcgcggcggg
    10561 tcgacgaata cgcagccgaa gtcgtagctt ttcaggtcga tgccggccag gcgacggaac
    10621 ggccgcacct cattcaacgc ttcggtcagt tcctcggcgg acaggcgcac caggctgaca
    10681 ttgtccacgc cattgtcggc caggttggcc agggcggcat tcaccgagga cttgctgatc
    10741 tcggtggcca gtaccttgcg cacgcgggtg gccagcggca gggtgaagtt gccgttgccg
    10801 cagtacagct ccagcagatc gtccgaacgt tcgccaagtg cgtcataggc ccagctgagc
    10861 atcttctggt tcacttcacc gttgggctgg gtgaacgcgc cctctggttg gcgatagctg
    10921 aaggtgcggc cagcgacctg cagcttttcc tcgacataat cccggccgat cacgatgcgc
    10981 ttgcccttgg agcggccaat gatgctgact tg
//
LOCUS       DASRUM010000201         2854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_102748, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000201 DASRUM010000000
VERSION     DASRUM010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102748"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFF22_13165"
     CDS             <1..193
                     /locus_tag="VFF22_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZX17797.1"
                     /translation="AIRAQLAAKSPELAGKVRILYGGSVKAASAAELFGMPDIDGGLV
                     GGASLNADEFGAICRAAGS"
     gene            198..581
                     /gene="secG"
                     /locus_tag="VFF22_13170"
     CDS             198..581
                     /gene="secG"
                     /locus_tag="VFF22_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HZX17798.1"
                     /translation="MLETVIVVLHLLGAIGVVALVLLQQGKGADAGASFGGGASSTVF
                     GSQGSTTFLSRVTAILATAFFMTSLGLAFFAKDKADGMAQVGLPDPAVLEVQQAAPAV
                     EDVPVLDAAKPAIEAADVPQAPEQQ"
     gene            614..699
                     /locus_tag="VFF22_13175"
     tRNA            614..699
                     /locus_tag="VFF22_13175"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:648..650,aa:Leu,seq:gag)
     gene            1001..1462
                     /gene="rimP"
                     /locus_tag="VFF22_13180"
     CDS             1001..1462
                     /gene="rimP"
                     /locus_tag="VFF22_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HZX17799.1"
                     /translation="MSSKLEQLQALVAPVVEALGYQCWGVEFLSQGRHSLLRIYIDKQ
                     PDGVLIEDCEIVSRQISGVLDVEDPISAEYTLEVSSPGMDRPLFTIEQFASHAGEQVK
                     IKLRSPFEGRRNFQGLLRGVEEQDVVVQVDDHEFLLPIDMIDKANIIPRFD"
     gene            1530..>2854
                     /gene="nusA"
                     /locus_tag="VFF22_13185"
     CDS             1530..>2854
                     /gene="nusA"
                     /locus_tag="VFF22_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HZX17800.1"
                     /translation="MSKEVLLVVESVSNEKGVPASVIFEALELALATATKKRFEDEVE
                     LRVAINRQTGNYETFRCWTVVEEADFDDPAHQVTTDMPRAVEANAKVGEVLEEKIDSI
                     EFGRIAAQTAKQVIVQKVREAERAQVVEAYRERLGEIISGTVKKVTRDSVIVDLGNNA
                     EAMLAREDIISRETFRVGVRLRALLKEIRTENRGPQLILSRTAPEMLIELFRIEVPEI
                     AEELIEVMGASRDPGSRAKLAVRSKDKRIDPQGACIGMRGSRVQAVSGELAGERVDIV
                     LWDDNPAQFVINAMSPAEVAAIIVDEDAHAMDIAVGADNLAQAIGRGGQNVRLASQLT
                     GWTLNVMTEADIQAKQQAETGDILQNFIDELEVDEELAQVLVEEGFTSLEEIAYVPME
                     EMLSIDGFDEDIVSELRARAKDRLLTKAIANEEKLADAHPAEDLLSLEG"
BASE COUNT          614 a          693 c          883 g          664 t
ORIGIN      
        1 ggcgattcgc gcgcagttgg cggcgaaaag tcctgagttg gctggcaagg taagaattct
       61 ttatggtggc agcgtcaagg ccgccagtgc tgccgagttg ttcggtatgc cggatatcga
      121 tggggggctt gtaggtgggg cctctctgaa tgcggatgag ttcggtgcga tctgtcgcgc
      181 cgcaggaagc taaagaaatg ctggaaacag ttattgttgt tcttcatctg ctgggcgcca
      241 tcggtgttgt tgcgctggtt ttgttgcagc agggtaaagg cgcagatgca ggtgcttcgt
      301 ttggtggtgg tgcttcttct accgtgttcg gtagccaagg aagtactacc tttctgagtc
      361 gagttactgc tatactcgcc accgcttttt tcatgactag cttgggttta gcgttctttg
      421 ctaaagacaa agctgatggt atggctcagg tcggtttgcc tgatccagca gtcttggaag
      481 tacaacaggc tgcaccagct gttgaagatg tacctgttct tgatgcagct aagccggcta
      541 tcgaagcggc ggacgtacct caggctcctg agcagcagta acacgcagta agttgtatgc
      601 aacaaatgat tttgccgagg tggtggaatt ggtagacacg ctaccttgag gtggtagtgg
      661 ccataggctg taggggttcg agtccccttc tcggtaccaa ttttcaagaa ggcccgctcg
      721 atgcgggcct ttttgttgct ggagcgtcga ttgaccctgg ttaaggttca gtcgtataat
      781 ccggccccag ctttgatgtg ggattgagca gtaaggtcgc tcctcggatg ttgaatccga
      841 agctggctgg gttaaaccag cccccgcatt gagttgcaga ggagcccctt ttaaggggct
      901 ttttgctagc tgggcgacag ctttgccggc ctttagaggg cggtttgatg atgggcgatt
      961 cgcccatttt ttatttttac agcatgcgcg aggggttcag gtgtcgagca agctagaaca
     1021 gttgcaggcc ttggtggccc cggtagtcga ggctcttggc taccaatgtt ggggtgtcga
     1081 gttcctgtca caagggcggc attctctgct gcgaatttat attgataaac agcctgacgg
     1141 cgtgttgatc gaggactgtg aaattgtcag tcggcagatc agcggtgttc tcgacgtcga
     1201 ggacccgatc agcgctgagt acacccttga agtgtcctcg ccgggtatgg atcggccgct
     1261 gttcaccatt gaacagttcg ccagccatgc cggtgagcaa gtaaagatca agctgcgttc
     1321 gccttttgaa ggtcgacgca acttccaggg ccttctgcgc ggtgtggaag agcaggacgt
     1381 ggtagtgcag gtggatgacc atgaattcct gttgccgatc gacatgatcg acaaggccaa
     1441 cattatcccc aggtttgatt gagacgcgga tcccccgcaa gtggcgcgga tagcgcggat
     1501 ccaatggatt gcgaaaggcg aggcgtacga tgagcaaaga agtactgctg gttgttgagt
     1561 cggtatccaa cgaaaagggc gtaccggcta gcgtaatttt tgaggcgctg gagctggctt
     1621 tggccacggc taccaagaag cgttttgaag atgaagtaga gttgcgtgtg gcgatcaatc
     1681 gtcagaccgg caactatgaa acattccgct gctggacagt ggttgaagaa gctgactttg
     1741 atgatcctgc acaccaggtc accactgaca tgccgcgtgc agtcgaggcc aacgccaaag
     1801 ttggtgaagt gctggaagag aagatcgatt ccatcgaatt cggtcgtatt gccgcgcaaa
     1861 ctgccaagca agtgattgtg cagaaagtcc gcgaagctga gcgtgctcag gtggtcgagg
     1921 cttaccgcga gcgtctgggt gaaatcatct ccggcaccgt gaagaaagtt acccgtgaca
     1981 gcgtgatcgt tgatctgggt aacaacgcgg aagccatgct ggctcgcgaa gacatcatct
     2041 cccgcgaaac tttccgtgtc ggtgtgcgcc tgcgtgccct gcttaaggaa attcgcaccg
     2101 agaaccgtgg cccacagctg atcctctcgc gcaccgcgcc ggaaatgctg atcgagctgt
     2161 tccgcatcga agtgccggaa atcgctgaag aactgatcga agtcatgggc gcgtcccgtg
     2221 atccaggatc gcgcgccaaa ctggccgtgc gttccaaaga caagcgcatc gacccgcaag
     2281 gtgcctgtat tggtatgcgc ggttcgcgcg tgcaggcggt ctccggtgag ttggcgggtg
     2341 agcgtgtgga tatcgtgctg tgggatgaca atcctgccca gttcgtaatc aacgccatgt
     2401 cgccagccga agtggctgcc attatcgttg acgaagatgc ccatgccatg gacatcgccg
     2461 ttggcgcaga caaccttgct caggccattg gccgcggcgg tcagaacgtg cgtttggcca
     2521 gtcagttgac tggatggacc ttgaacgtga tgaccgaagc ggacatccag gccaagcagc
     2581 aagcggaaac aggcgacatc ctgcagaact ttatcgacga gctggaagtc gacgaagaac
     2641 tggcccaggt gttggtcgaa gaaggcttca ccagccttga agaaattgcc tacgtaccga
     2701 tggaagaaat gctcagcatt gatggctttg acgaggatat cgtcagcgag ctccgcgccc
     2761 gtgccaaaga tcgcctgctg actaaagcca tcgccaatga ggaaaagctg gcagatgccc
     2821 acccagccga agacttgctc tcgcttgagg ggat
//
LOCUS       DASRUM010000202        63018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_621213, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000202 DASRUM010000000
VERSION     DASRUM010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 63018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63018
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621213"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VFF22_13190"
     CDS             <1..607
                     /locus_tag="VFF22_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042650166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HZX17801.1"
                     /translation="CGISKNTAFLWRHRFLACAAQHHAAHETGIVEVDETFFLESFKG
                     QRKLPRAPRKRGGVGKDQIPVMVVRDREGHTADFKLEKLDAEHVRAALLPLIDREAVL
                     CSDGAAVYANFARTQGITHHVVHSRPGERVRQGAFHIQNVNAYHSRLKSWMTRFHGVP
                     TRYLPNYLGWRRMLERYQQRIQQVHCIQEAIGRTMQHAIGT"
     gene            complement(662..1615)
                     /locus_tag="VFF22_13195"
     CDS             complement(662..1615)
                     /locus_tag="VFF22_13195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HZX17802.1"
                     /translation="MNELLNVVVHKRELQGDGVVVIDLLLKNGGNLPAFEAGAHVDVH
                     LPSGHTRQYSLCGDPANAAIYRLGVLKDPQSRGGSVGVHDALHEGVAVDISYPRNHFP
                     LDLTAHRSILIGGGIGVTPLLAMAYALKAQGKTFELHYCGRSRSRSAFLDELERSDFA
                     DVVFTHFDDEPESKLNLETVLGQAQEGTHLYTCGPSGFMDWVINTALTAGYPDTQIHR
                     EYFQADVDNSGASFEVVAARSGKTVTVEEGQSILQALAGVGIDIEISCEQGVCGTCLC
                     DVIEGEPDHRDVYFTEDEKAANDAILVCCSRSKSAKLVLDI"
     gene            complement(1760..3268)
                     /locus_tag="VFF22_13200"
     CDS             complement(1760..3268)
                     /locus_tag="VFF22_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZX17803.1"
                     /translation="MALANNYDNLSAGAQSAIGKRTQLFINGEFRNPLSGQYYPIVDP
                     SNGLELAEIAQADKHDVDFAVSSARKAFEDSAWSKMKPYQREALMLRLADLLTENAQE
                     LAELETLSSGRLIRNTRAFDVDFSVYTLRYMAGWSTKISGKTFDLSVPYLPDQQFSGF
                     TKRAPLGVVAAITPWNVPLCQAIWKLAPVLATGCTIVLKPAEQTPFTALRLAQLCNEA
                     GIPPGVVNVVTGLGHEAGAALVEHPGVNKISFTGSTETGKRIAAVAAPLLKKYTLELG
                     GKSPVVIAEDADLNIAIPGAAWAIFGNHGQNCCAGSRLFVHERHYAEVLAGVAEIARS
                     IKVGPGLDLSSQMGPLVHNAHRDKVLGMIKQGLESGAEVLCGGEAISGPGAYIQPTVL
                     TGAGHDDQIVQREIFGPVLTAFSYKDDLEVIQRANATSFGLGASIWTKDVDRINTFFS
                     RMKVGTVWINNHNVLDLSMPFGGVKDSGFGHELGEEGLLDHTILKAGVMRTF"
     gene            complement(3880..4647)
                     /locus_tag="VFF22_13205"
     CDS             complement(3880..4647)
                     /locus_tag="VFF22_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZX17804.1"
                     /translation="MTLKIAQRWFERKTFSDGITLLWEPHVHPMLRCNIWHVRGRTRD
                     LLIDTGMGVASLRDAACDLFEKPLAAIATHSHLDHMGGLHEFDCRLCHAAEAALLANP
                     SQSDRLNLQPDHWLALYMPDLDDSRLVIDALPYEGFDPLAWRLQPAPPTALLADGDML
                     DLGDRHFEILHLPGHSPGSIALFEKHTGILFSGDVIYDGDLLDEIPGGDRAHYIRSLQ
                     RLCELPVQAVHAGHEGSFSGERLRALASVNLELWQRG"
     gene            complement(4651..5733)
                     /locus_tag="VFF22_13210"
     CDS             complement(4651..5733)
                     /locus_tag="VFF22_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019579230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZX17805.1"
                     /translation="MTLALISGGAGAAETALQRSVNVYGWADYIAPQTLVRFEQQTGI
                     RIHYDTFDSADMLEAKLLTGRSGYDVVMPGVAMLDRLIRADALQPSQMQHYPQAAEYD
                     PQLLSQLAAVDPGNRYAVPYAWGTTGLAFIREAIEQRIPNPPLNSFDLLFKPEYASKL
                     KDCGIAIIDSPQEVLSIALHYLGKNPYGDSSAELAQAGQLLNELRPNLRYIAFGKQAS
                     DLANGNLCLALTYNGDAVTANAQAIEAGQAHTVEYRIPREGTLAWIDTLAIPADAPHP
                     QEAKALIEFLTSATAMAELTNAIFYANANSTSVAQITQTLRENTTLFPPSNVRQTLFG
                     EEPLSAKTLRQRTREWARFRSQVSQE"
     gene            5888..6778
                     /locus_tag="VFF22_13215"
     CDS             5888..6778
                     /locus_tag="VFF22_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007943017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZX17806.1"
                     /translation="MGLPPLNSLRVFETVARLGHLGAAAAELHVTPGAVSQQIRALQI
                     NLGVELFHKQGRHLVLTPSGLVLQRAVARGIEEISQGVRQVLTEVQVAAPDTVLTIST
                     EPVLGTSWLLPKLFAFRAQHPNIHLRVITASTASEVDWKKADIALLYASPPWEGLWWR
                     PLRRLHMFPVCCPQLLPQVRQPADLTNQRLLHEDDGALWRRWLVAARVPYPGDADVFI
                     QSFPMALQAARDGYGVALSDEFNSHRDLAEGRLVRPFTLQVPGGLDYYCLSLEERREL
                     PEVVQLIDWLIEEAGRDVCL"
     gene            7070..7891
                     /locus_tag="VFF22_13220"
     CDS             7070..7891
                     /locus_tag="VFF22_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003209869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis F family
                     protein"
                     /protein_id="HZX17807.1"
                     /translation="MSTKSDWITVGALADGFAPAAFILPNLADLAGKTFTLNFANGWQ
                     ITHAFEAERLSWVAADGHSQGSATYRATSVRPGIYLVDFLKNENGQDWSISLVLDIDS
                     ASFTAVIGRLPVEQTTHEGLYHRALAGKQLTGVEVEFFHGSLDKPWQEGACRHALTHE
                     LVGLRNLYRYSPTEVYEHIYLNENFYSWQCIKGVEAGLCDTDRCHYYKIADELYLFVW
                     REKIVPTLGLVMIDLANHRSDGKIFGYAGGGFDELSNFPVASYCSVLNRTEYPDE"
     gene            7884..8678
                     /locus_tag="VFF22_13225"
     CDS             7884..8678
                     /locus_tag="VFF22_13225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003182214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX17808.1"
                     /translation="MSEPRTIVITGAGTGIGEACARRLAGEGHNLVLIGRRRQPLERV
                     AALTGGLVLVGDAASSAIWSGFVDEIRARFGGIDALLACAGGHGLGSATETSDEVWQA
                     ALRSNLDSAFHSARACLPLLVERRGSIVLFGSIASLAAGPQVCGYTTAKHALLGLNRS
                     LARDYGPLGVRVNCVCPGWVRTPMADEEMQPLMAHFGEDLDAAYARVTADVPLRRPAS
                     AEEIASVCRFLISVEASIITGASIVADGGSSIVDVPTLVYDRLGGA"
     gene            8678..9448
                     /locus_tag="VFF22_13230"
     CDS             8678..9448
                     /locus_tag="VFF22_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011534266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX17809.1"
                     /translation="MGTQFDFTGQTVLVTGGAQGIGRGIVEGFAASGARVLIADLQIE
                     PAQALARELQGNGCEAQALGIDLADEVAIAALLGGLERLDILVHNAGYFPLTPFAEIT
                     PAILQRTLAVNLSALFWLTQGALPLFERQGRGCVLVTSSVTGPRVAYPGLSHYAASKA
                     GVNGFIRSAALELAKCKVRVNGVEPGMIRTPAMGNLGDGDHSADIARGVPLGRLGEPE
                     DIAGAMLFLASEQASYITGQTIIVDGGATLPETQAALD"
     gene            complement(9536..10495)
                     /gene="feaR"
                     /locus_tag="VFF22_13235"
     CDS             complement(9536..10495)
                     /gene="feaR"
                     /locus_tag="VFF22_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003173206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HZX17810.1"
                     /translation="MHAQHVTHSALENWQRSMQSVCGNYETRLAFNNALFIGDVCAKE
                     HAGLTLAHLKTNAGLISRNAKSDRDDDRYCFLVSQRSGCSRITQNGVTLELTPGDLVL
                     MDSVGSCEITPLGLIEHASLHLPREQVLKSFQCDRQLFGKASAHCSSGRMLRLLVDQL
                     YMNEEGTHTDDNETESMLSAFTSLLVPALVQRENQAEIADSIAGNSLRRQAQQLIEES
                     LTQPNLNPAALANRLHISVRQLYRLFEEQGDSVCRYIQRTRLQRSASDLSSPHLRRES
                     ITAIAYKWGFTDSAHFSRAFKKQFEQSPKDYRAGVFMELGSAH"
     gene            10941..12266
                     /locus_tag="VFF22_13240"
     CDS             10941..12266
                     /locus_tag="VFF22_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018927905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HZX17811.1"
                     /translation="MHQERRCLSISKAVCLAVAALAAGQSQAYEIYNQGETKLNADFL
                     AVYGWFNSRKSYDGSSGGASWREGFAKYGISAEQGLAGNGTAYGALNWVSSGTWGDGD
                     AAGLSDGSERTTKIEDAYLGWRSGNLLPALGENGLDISFGRQVVTIGDGFIINDDGLN
                     FGKGVADGELNRGGGYYLAARRAFDQTAVVRIGGEEGLHGDLMWLKSDNRAQAKTELA
                     ATTLAYTAEPGTLAMTYIHGIDVDDQYASESQQQRDGMDIYSLRGAGNAGVENANFAF
                     EYAWQDKDAGDEKAWYVEAGYTFADVAWSPNLTYRYSRYSEGWDSMFNGFNRGYGTWF
                     QGEVAANYAGPFNSNTRIHHVGFKVNPLETLTLGALYFDFDTLNTRDNPNMGGRELDL
                     YAEWVVTDNLIISPLIGFYQPDKSLENGGLQGGGNGTNVYSQLTVAVPF"
     gene            complement(12732..14914)
                     /locus_tag="VFF22_13245"
                     /pseudo
     CDS             complement(12732..14914)
                     /locus_tag="VFF22_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715977.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(15276..15791)
                     /locus_tag="VFF22_13250"
     CDS             complement(15276..15791)
                     /locus_tag="VFF22_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HZX17812.1"
                     /translation="MRKLIIGLVCAMVAPLAIARAIPNPDQRPAPGNEEPQKPIAQAH
                     YSTPVNYQLQCAGCHLDDGEGSKANDTPRMKGFVGNFLKVEGGREFLVRVPGMSQSAL
                     SNAQLADLLNWLLKTDGIAGGSTPEHYRPYTEEEVATTRKVSLLNLPDVRADLIKKMV
                     EQGIEISDGMD"
     gene            complement(15807..16328)
                     /locus_tag="VFF22_13255"
     CDS             complement(15807..16328)
                     /locus_tag="VFF22_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine dehydrogenase light chain"
                     /protein_id="HZX17813.1"
                     /translation="MKLLDRLFERSTRHVADTTSRRKFLGRLGSLMVAGAALPVLLPI
                     DRTSKALAAEAPKAGDPGDPNSCDYWRYCSIDGFLCSCCGGSVTSCPPGTEVSQVTWI
                     GTCRNPGDGKDYIIAYNDCCGKHSCAQCACTRNDSEEPLYRPFNNNDINWCLAAKSNI
                     YNCTVAIIRGVTV"
     gene            complement(16345..16956)
                     /gene="mauD"
                     /locus_tag="VFF22_13260"
     CDS             complement(16345..16956)
                     /gene="mauD"
                     /locus_tag="VFF22_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine dehydrogenase accessory protein
                     MauD"
                     /protein_id="HZX17814.1"
                     /translation="MDALIVSNILLWGLLIALAFAVMGLVRQIGVLHGRLAPAGALMV
                     DKGVAVNEPAPQVTAADRSGRPVNFGYAGEKAQLLFFLSPTCPICKSLLPAIKSIAKE
                     QAHRLDVIYVSDGDMDAQKALITEHKLDDATYVVGPEVGMTYQIGKLPYAALIDNAGV
                     LRAKGLVNSREHLDSLFETEHLGSATLQQYLHNSQHAHGAHNH"
     gene            complement(16960..17499)
                     /locus_tag="VFF22_13265"
     CDS             complement(16960..17499)
                     /locus_tag="VFF22_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HZX17815.1"
                     /translation="MQPDPIFVIAAALAVAVILASAATHKLRAPARFANQLEAYQLLP
                     QALVSPIARLLPLLEAGLAFALLVPAARHFAALGAAALLASYALAIGINLWRGRRDID
                     CGCAGPQQEQPIRPLLLARNTVLVSLALVASLAPLSRNLGLFDAFVVIAASAVALLIY
                     AAADGLMANSPRLLKLIGR"
     gene            complement(17512..18660)
                     /locus_tag="VFF22_13270"
     CDS             complement(17512..18660)
                     /locus_tag="VFF22_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - amine dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amine dehydrogenase large subunit"
                     /protein_id="HZX17816.1"
                     /translation="MQIPWNIRQTALALGLSLAGLSANAELPRDTIGQETLPFPPSPH
                     RAYIVDAEFENLVTGRITVVDPDSKRMLGMVSTGFAAPSTLSNDGKHLFTADLFYSRG
                     TRGTRTDVLTAWNTSTLSPDWEVVIPSTRAVMLPERHALGTSSDDRFVYIYNFTPSTS
                     VTVVDTQARKVISEISIPGCVLNYPVGKRSFASLCGDGNLQVVTLDDTGKEVARTHTA
                     FFDPNAEKLVERAYAVGDTYYFVSTTGTVRAVDFSGEQPKIIPAWELVSDAADKKAGW
                     APGGWQLIAVAPKLNRLYALMHDAHEPLKWEDPSTIIWVYDLKTRKRIGTLEAPAPIW
                     SLQATTDDKPRLLGANVVGGLEVFDLTSGKHTGTVERIAKTAIHILSH"
     gene            18982..20307
                     /locus_tag="VFF22_13275"
     CDS             18982..20307
                     /locus_tag="VFF22_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008033879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17817.1"
                     /translation="MSKLPSFHLTTLALLIGTALPAAAVELYADADTRLEGNLEAAFG
                     MFHSRENYAASGRLQEGSSSWREGYFKYGLSFDQGLGAVGSSYGAFSLLSSATWGDGD
                     AAGFSDGSERTTKIEDAYLGWRSGELFEALGENGVDLSFGRQNVMVGDGFLINGDALN
                     IGNGLADGEFNRGGAYYLAARKAFDQTAVLRLGGEQGWRGDLMWLKSDNRAQAKTELY
                     VGTLEHVADVGTVGLTYIRTRDVDEQYASPSQLQRKGMETYSLRAAGNAGVESLALSG
                     EYARQDRDQGSDENAWYLEAGWTSADVAWSPTLSYRYSRFSEGYDPLFYGFSRGFGTW
                     FQGEVAGNYAGPFNSNSAVHHVGLRASPLENLSVGVLWFGFDTLDRERGDFSGRELNL
                     YAELGLTENLVVMPVVGLYQPKKSAEEGGAQLGNNDRNLYSQLVFAFNF"
     gene            20436..20912
                     /locus_tag="VFF22_13280"
     CDS             20436..20912
                     /locus_tag="VFF22_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZX17818.1"
                     /translation="MKVPKKRRNRYNPISEEFHKEEFPFYWLVRVHARYTMSMERLLK
                     KIDLDIPRWRVLNILYEAQEASISDISEFAVAKLSTITKIVYRMKDDGLVDTRQSDQD
                     GRVTQVSITDKGREAYFAMHEATAQVFHDGFRGLTEAQIRKLNEILATMFDNLSDS"
     gene            complement(20924..21859)
                     /gene="feaR"
                     /locus_tag="VFF22_13285"
     CDS             complement(20924..21859)
                     /gene="feaR"
                     /locus_tag="VFF22_13285"
                     /inference="COORDINATES: protein motif:HMM:NF007243.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HZX17819.1"
                     /translation="MTVHSSHAFERWTHGVHSVCGRFETQQTWGADFIGEIQRIDLGG
                     LEIADISTNAQAINRQRGNTARADDRYYFLVMQREGVMAIDQGDREFVLYPGDMALLD
                     SAQAFEMKPQGLIRQLSVHLCRDMVDPLLPNSARRFGKLEQESPTGHLLQGILQQIAN
                     GELNSATQRPYGSALQNALVALLPPAFNDERLLDPGRPLRRLAEKIINESLPNAPTPA
                     ELAAKMNVSVRKLYRQFEIDGDSICRYIQRQRLERSARELADTSSHALPITTIAYKWG
                     FTDSAHFSRVFKQHYGISPRTYRADASTRQQVMLG"
     gene            complement(22093..23580)
                     /locus_tag="VFF22_13290"
     CDS             complement(22093..23580)
                     /locus_tag="VFF22_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010446606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZX17820.1"
                     /translation="MSEIQLLPQVREFLQRDHAHFIDGEYRAGTSGKRVPIVNPANGE
                     VIAQITCATSAEVEAAVAAARRTFEGEWAQTSPYQRGVILNRLADLMEANGEELAQLE
                     TLCSGKSIHLSRMFEVGQSAIFLRYYAGWTTKINGETMTPSFPSMAGEQYTAFTRREP
                     VGVVAGIVPWNFSVMIGIWKIAAALATGCTLVLKPSEFTPLTLLRLAELAIEAGVPAG
                     VFNVLNGTGEVGASLIEHPQVAKVSFTGSVPTGIKVGQSAMAANLTRVTLELGGKNAA
                     ALLPDVDIDSAVAGLIQTGYVHQGQVCAAPERIYVHRSRLDETLQKFSTALQGMNIGS
                     PLDESVNFGPLANKPQFDKVAQFLQLAHEESQVVCGGKPLARPGYFVEPTVVLAKGPR
                     DRLLHEETFGPILCVLPYDEVEELPGLINDSPFGLTASIWTNDLSRALRLIPQIEAGT
                     VYVNMHTFLDPSVPFGGVKASGTGREFGSAFIDDYTELKSVMIRY"
     gene            complement(23773..24834)
                     /locus_tag="VFF22_13295"
     CDS             complement(23773..24834)
                     /locus_tag="VFF22_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZX17821.1"
                     /translation="MKLLFALLLLFASTAHAHAQEVVRVYGWKDYIDPQVLEDFQAHS
                     GITVDYQSFTTTHELLEALAAGTDYDVVMPSHFMLDQLIAGGHLTPLDTRRLRHYTSL
                     DPWLLSTLAGIPAANRHAVPYLWGSIGLVVDPQQARAHYAGTLPNSWRLLFDPEQAAK
                     LSPCGLGMLDAAEETGSLLLNYRGRRLSASSNRQIARQLRGLETIAPLLRTLDNWNFV
                     DELIAGRLCLAMAWSGHAARAMEGNPQLRYQIPDEGAAMYIDTLAIPTHAAHPQLAYR
                     LIDFLIDPTVAIRNARATHFYPPLPSASPLMQAFASEHPVQVLTAEQRRRSYLLESLS
                     PQQKQVLDEAWARVRAARH"
     gene            complement(24972..26528)
                     /locus_tag="VFF22_13300"
     CDS             complement(24972..26528)
                     /locus_tag="VFF22_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX17822.1"
                     /translation="MQTANNTPPGFDEPPISRLDPQGVLLTCNAAYLHMSGFTEQELI
                     GKAHEQVNHPHMPASVIASMWRALRAGVPWTAPVMIRHKQGNRDWHNLYVVPLFDDGK
                     LVALGTVYQPIEPELARRAESLYARLAQGQAPWKPVQHCAALLAGNGLQLVLAVGLAL
                     VTLQGYLSAGVGVPLLLALGLAGLQPVLHQRRLSRTLLAQHAQVYSDPLLTPLYAEQP
                     GPAGLLAMALDSQRARMTTVMSRICINSVVLRQQAQTSADVVEHSVEQLQHQVRETEQ
                     IAGAINQMSATIQELSRNLQHTAQAAQNAEQLARDGERMSDDSQRSAQAMHNSVGEIG
                     QAVGSLAAAIESIGSVAEVIQSIAEQTNLLALNAAIEAARAGESGRGFAVVADEVRSL
                     ASRTRESTHEIQRSLESLRHDSGTALATAQRGEVAALRASTDVQQARQALTRICDEIT
                     QISGMNLQMAAAIEQQGQVAEQINQQITDIAGFTERTSQQAGRTNEISQALHQLAESQ
                     LGLARRFLNG"
     gene            complement(26597..27382)
                     /locus_tag="VFF22_13305"
     CDS             complement(26597..27382)
                     /locus_tag="VFF22_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX17823.1"
                     /translation="MNMPTQQHAQNQVANPVLEVSDLCVAYSKVEALSNASLRVGQGQ
                     IVTVIGPNGAGKTTLLSAIMGVLGSRGRVAFDGSLEAVPEVEVMVGRGLGLVPEKREL
                     FSSMTVADNLLLGAFQRHRSGQRDHGQTLKEVYELFPRLWERRGQLAATLSGGERQML
                     AVGRALMAKPKLLMLDEPSLGLAPLITREIFRIITALRQQGVSILLVEQNARAALRVA
                     DYAYVLETGQIAMQGPSAQLADDPRVIEAYLGLASKHQDMLAT"
     gene            complement(27382..29181)
                     /locus_tag="VFF22_13310"
     CDS             complement(27382..29181)
                     /locus_tag="VFF22_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HZX17824.1"
                     /translation="MKPRYLILALIAVLAVAPAVLPPYYLTLLNYIGLYTLVVLGLVL
                     LTGVGGMTSFGQAAFVGLGAYTSAYLTTVQDLPSWLAWTSASPWLSLLVGLTLTAVVA
                     LVLGALTLKLSGHYLPLGTIAWGLSLYYRFGTLESLGGHTGVSGLPSISLFGWVLDKG
                     EKIYYLIWAILLGAMLLTQNLLDSREGRAIRALKGGQLMAESMGVNTFRTKMLVFLIS
                     ALFAALSGWLYAHTQRFVNPTPFGLHMGIEYLFMALIGGVTSVWGALLGAGALTLLKQ
                     WLQDLLPQLLGNTGNYEVIVFGIAIVLLMQRAPGGLWPLFTRLLPGSWKARRAALPVD
                     DASALPQREAPVVGEVLLEARDVTRCFGGLVANNEMNLEVHAGEILALIGPNGAGKST
                     LFNQLSGVDTPTSGEVLFRGQRINGIPSRRIAHMSMSRTFQHVKLLPEMSVLENVALG
                     AHLRGSKGVLAAALRLDRAEEARLLAEARRQLERVGLGDCLYLEAGSLALGQQRLLEI
                     ARALCANPCLLLLDEPAAGLRHKEKQTLGQLLSRLRSEGLAILLVEHDMDFVMGLVDR
                     VVVMEFGQRIAFGLPADVQKDPAVLEAYLGGAE"
     gene            complement(29178..30215)
                     /locus_tag="VFF22_13315"
     CDS             complement(29178..30215)
                     /locus_tag="VFF22_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZX17825.1"
                     /translation="MNVQIALLLGQDGITNGAIYALLALSILLVFTVTRILLIPMGEF
                     VTYGALTMAALQAGQSTALVWLLLAMTLADCAMDLYDAARASRRFRLPRRIALKLAYA
                     LTMLGLVHNLPLAQLPMAVQALLTLALVVPLGPQLYRLVFQPLAAASALVLLIVSIAV
                     HVAMVGIALLLFGPEGARTEPFSEAGLTLGPVTFNSQTLWVIAVSLTLIISLFLFFER
                     SLYGKALRATAVNRMGARLMGISPNLAGKATFAMAAFIGALSGILIAPITTLYFDSGF
                     VISLKGFVGAIIGGLVSYPAAALGALAVGLIEAFSMFWASTYKEIIVFTLIIPFLLWR
                     SLTSRHVEDDE"
     gene            complement(30366..31517)
                     /locus_tag="VFF22_13320"
     CDS             complement(30366..31517)
                     /locus_tag="VFF22_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX17826.1"
                     /translation="MHFKRAVSSLLLSAVCASFVQAEVKVGVITSSTGPIALVGLPQK
                     NSIALLPDQAGDQQVKYIALDDGSDPTATVKALKKLISEENVDVIIGPSGSPNAMGVI
                     QFVAEAGVPLLAPVGTAAVVLPMTEQKKWVFKTTQNDDLIAKALVEDMNARGVKTLGF
                     IGTADPYGENWSKVMAALAAEHGIKMLASERFQRQDTSVTGQSLKILASRPDAVLVAA
                     PGSAAVLPQTTLFDQGYRGQMYQTHGAALPDFLRLGGKKVEGTILAASLMLVLEQVPD
                     SHPSKAIAAEYVAAYEKLNGSQPATFGANTYDAGLLLQQAIPAAAAKAEPGTPEFRSA
                     LRDALEATHEFAATQGVYNMSPEDHSGFDERGRELIQVKNGNWTLLGSH"
     gene            32220..33332
                     /locus_tag="VFF22_13325"
     CDS             32220..33332
                     /locus_tag="VFF22_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZX17827.1"
                     /translation="MAIVVEKLQLGGQGLRVMVKDTIDVAGHPTRASSRALQQAPRAE
                     RHADVVQALLDAGCQLLGKTSLHELAFGTTGLNAWTGTPENPRYPGRIPGGSSSGSAA
                     AVAGGLCDFSLGTDTGGSVRIPAACCGVFGLKPTFGRISRAGVMPAQTSLDCVGPFAA
                     DIETLIAAMSAIDPSFKTLPAVNGICIGVVPVEAHPEVQALLGAVLEASAFDLRSVPL
                     PGMQAAYDAGLAVINRETWNACGHLLETGLVGSDVAARLLAAGDTSEAALADAERCRA
                     AFTAEVDGALRQCPILALPTMPEYPALVAEAADTRAAIGMTAFVRPFNLTGHPALSIP
                     FVGASLLPVGLQLVAAKGADELLLAVARELLQRLEQ"
     gene            33356..34528
                     /locus_tag="VFF22_13330"
     CDS             33356..34528
                     /locus_tag="VFF22_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZX17828.1"
                     /translation="MSSLSQLDHQRLAPITANEAFQALLTDIRERARREEFDQQRFIS
                     HDVIERFKALGVYRALVPTRFGGEQRSPMEFCEMVEEVSAADGSAGWVASFGMNPVYL
                     AALPLATLDKVYANGPDVVFAAGIFPPQPAETIEGGFKIRGRWKFSSGCKGADLVGVG
                     ISPKVGETHSLPRVAVVPQAQVVIDETWDMVGLIGTGSHDLVIDGMEVSEEWTFVRGG
                     QANLDEPLFRYPSLSFATQVLSVVGLGVARAALDYLTDMASGRFSVTGAPVLADRPLA
                     QMQMAKAEAELRAARSWFYEAMENAWASVLAGDAVSTEQTNLLRLSSTHAVRVSAEVA
                     RSAQMLAGMTGVCRNSPLARFVNDAQVVTQHAFMGDMTYQNAGAIFFGGKPMPGYL"
     gene            34555..34917
                     /locus_tag="VFF22_13335"
     CDS             34555..34917
                     /locus_tag="VFF22_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IacB protein"
                     /protein_id="HZX17829.1"
                     /translation="MTEKKSLRVLFCMGINQNFFDAAREEQLEVWAAFSAMWNGIHDL
                     PGVTVYGNMDDDQSMVGPSTGYPWTTYLLADVPDIETVHAACNLFRTTAVGAGPYKLW
                     RYCKVEARTGRELIIQRA"
     gene            34921..35436
                     /locus_tag="VFF22_13340"
     CDS             34921..35436
                     /locus_tag="VFF22_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZX17830.1"
                     /translation="MSRTLEARLHQLESENAVRACMNRYMVLCDALDARTPLDELAGL
                     FSADAIWEGKGAKYAKSFGGYRGREAIRAMFAGYMSEPAHFALNVHFLCSELICVEGD
                     EASGSWVMLQTSTFATGASHLNAARLTVRFREEEGAWRMAHFQTENLFGRPVDAWNSD
                     AHLPVPGQRDE"
     gene            35475..36749
                     /locus_tag="VFF22_13345"
     CDS             35475..36749
                     /locus_tag="VFF22_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZX17831.1"
                     /translation="MTNLIETVNLASSPADLVLHDRVHTSLYTDARLFDEELQKVFYS
                     TWVWVAHASEIPDNGSYKSTYIGKQPVIVVRDRKKDVHVLLNRCRHRGATVCEHKKGK
                     TNSFVCPYHGWSYALDGSLRGVPHPESYDDCLDKGELPLVSLRVEQYNGMIFATFKDD
                     IEPLVDFLGPAKKWIDLFMKQGAGYGVKVSGEHRFRFPGNWKIQLENTTDAYHFPLVH
                     KSFLSSVDEQTLELFDFVKGPGYVEDLGNGHSVMVMIPDLVDLEADLDKPIPERFEAL
                     AQELRDEGIDEQQVRRIVRAVGGSGFNLNLFPNVACSMAFFRVLQPVSVDETEIHHAV
                     ITMDGGPAVANRYRLRLHEHFQGPMGFGTPDDSEAWERVQKGTQAGSDLWIMLNRGLP
                     GEKPSEDGLVSDVSAETGMRAAYQQWKKMMSA"
     gene            36829..37305
                     /locus_tag="VFF22_13350"
     CDS             36829..37305
                     /locus_tag="VFF22_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HZX17832.1"
                     /translation="MNLELLNHVSAFIWQEADMLDHGEFDAWLKLWADQGTYIIPIDP
                     NETDFENTLNYAYDDHHMRELRVQRLIGGESISTSPRARTVRSLSRFRVLGETDGVVT
                     VRCAQNLREFRKDVLKHYTADITFQLKRDGASFKIQRKLIQLINSTDTLTGIGYIL"
     gene            37316..38056
                     /locus_tag="VFF22_13355"
     CDS             37316..38056
                     /locus_tag="VFF22_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX17833.1"
                     /translation="MTQVALVTGAAQGLGLAIANRLFAAGYAVVITDLSLQRAQAAVA
                     QLDPSGQRSLALQLDVASKADFEAALAAVLARWGALHVAVNSAAMTRTTPVMQISPEE
                     FDQVMSLNTRSIFLGCQVLGGYMAGAGYGRIINMASLAGQNGGTATGAHYAASKGAIV
                     TLTKIFAKELAAAGVTVNAIAPGPIESPAVHAAVPPERMAGLLANIPVKRLGDADFLG
                     DLVVQLARPEAYFTTGATWDVNGGLFMR"
     gene            38177..39136
                     /locus_tag="VFF22_13360"
     CDS             38177..39136
                     /locus_tag="VFF22_13360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HZX17834.1"
                     /translation="MTEHVLNLVVRKRQEQGEGVVILDLADPMGSNLPAFEAGAHVDI
                     HLKPGLVRQYSLCGDPANAAVYRLGVLRDPASRGGSVAVHDLLTEGASVSVGTPRNLF
                     PLAAGASRSILIGGGIGITPMIAMAHQLTAQDSPFELHYCGRSRHRTAFLDELASADF
                     AACVHTHFDEEDAAQKLDLTAVLGQPDAGLHLYVCGPAGFMDWVIEGARKAGYAEEQI
                     HREYFQVEVDSAGESFEVVAQRSGKSVQVAEGQTIIDALAGIGITVEISCEQGVCGTC
                     LCDVLEGEPDHRDVYLTDDEKTANDQILVCCSRAKSKKLVLDI"
     gene            39154..39654
                     /locus_tag="VFF22_13365"
     CDS             39154..39654
                     /locus_tag="VFF22_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HZX17835.1"
                     /translation="MIDNTGFRNAMALLGGAVSIITTDGAAGRWGFTASAVCSVTDQP
                     PTLLVCMNRSSFSNIHFKQNGVLSVNVLSAELKDISGVFASRDLDSDQRFAQASWSTL
                     ESGAPLLDDALVNFDCRIAQAHEVGSHTIFYCEVLGIRNGKSQDGLVYFNRAYHRLGE
                     QSRSAC"
     gene            39981..41210
                     /locus_tag="VFF22_13370"
     CDS             39981..41210
                     /locus_tag="VFF22_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015802 - basic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family porin"
                     /protein_id="HZX17836.1"
                     /translation="MAVFASISAQADFINDRHVSLGLRNFYIDRDFKQHDAPKSRIGS
                     WTQGFDFRSISGYTEGTLQFGLDLSAQYAYRLDGGGGRGPDSIIPYDDRKGEQVRDYG
                     RAALTAKVRYSKTELKIGEHRPMLPVAFYDDSRQLITTYHGFMLESREVDRLTLTAGR
                     FTEISSRESSNREQMYLFNGPDIQRRSDGLNFAGATYAFTPALSASYFYGQLEDIYQQ
                     QYLGGTYNADLGNGFGLKTDLRYFDNREDGKALYGDIDNRSYGVMTTLRKGAHAIGFG
                     YQRMLGDSTFPTLNGYVPQPYLVNWSTVAFVKPNESSWQLRYDLDFASYGVPGLKLMT
                     RYLRGSGIDRGNNDLAQNMESERNIVLGYVVQDGPLKGVAFEWRNIDVKTRYGNGAAS
                     GADYQENRLITSYTFTF"
     gene            41271..41894
                     /locus_tag="VFF22_13375"
     CDS             41271..41894
                     /locus_tag="VFF22_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HZX17837.1"
                     /translation="MRLYELITFTLRVRTPAEALQRLRETLGEARDGCVLMGCWASEI
                     GPLNQIAVLRGFRDETARQHERERFLQAPDAFGIETFMLDMKVENYTLFPFLEPLAPG
                     KHGPFYELREYNLIPSGLAPTMAGWEKAVGPRTGEGYSAVYAAFFATDGRTPRYLHIW
                     PYATQEQRLDVRTRAVADGVWPPENSGPQLREMHSTLYLPAVFSPLQ"
     gene            complement(41964..42848)
                     /locus_tag="VFF22_13380"
     CDS             complement(41964..42848)
                     /locus_tag="VFF22_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX17838.1"
                     /translation="MELRHLRYFQALAETLNFTRAAERLHIAQPPLSRQIQQLEDELG
                     VSLIERGRPLRLTEAGRFFHEHSSTLLEQLGKVCESTRRIGTGEKAWLGIGFAPSTLY
                     GVLPELIRRLRSDVDLELGLSETTTVQQVEALKAGRIDIGFGRIHIDDAAIHQQVLTL
                     EPLVAALPAGHPLLPGPVSLTELAREPFVLYPGNPRPSYADHVMALFAAHGLQIRVAQ
                     WTNELQTAIGLVAAGIGVTLVPASVQVLHRADIDYAPLQEAQATSPIIISRRVGDASP
                     ALEHCLALVDELSRARQG"
     gene            42975..44096
                     /locus_tag="VFF22_13385"
     CDS             42975..44096
                     /locus_tag="VFF22_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muconate cycloisomerase family protein"
                     /protein_id="HZX17839.1"
                     /translation="MSHAAIESLEAIIVDLPTIRPHKLAMHTMQNQTLVILRLRCADG
                     IEGIGEATTIGGLSYGNESPDSIKVNLDRHFAPLLIGQDASNINACMQRIDRVVKGNT
                     FARSAVETALLDAQGKRLGLPVSEILGGRVRDSLEVAWTLASGDTRKDIAEAEQMLEA
                     RRHRIFKLKIGAGEVNQDLAHVIAIKQALGERASVRVDVNQAWDEAVALRACRILGDN
                     GIDLIEQPISRHNRGGQVRLNQSSPAPIMADESIECVEDAFNLARDGAAPVFALKIAK
                     NGGPRAVLRTASIAEAAGIGLYGGTMLEGSVGTLASAHAFITLSKLTWDTELFGPLLL
                     TEDIVTEPPLYRDFHLHVPRLPGIGLTLDEERLARFRRP"
     gene            44119..44409
                     /gene="catC"
                     /locus_tag="VFF22_13390"
     CDS             44119..44409
                     /gene="catC"
                     /locus_tag="VFF22_13390"
                     /EC_number="5.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016159 - muconolactone delta-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase"
                     /protein_id="HZX17840.1"
                     /translation="MLFHVKMTVKLPLDMDPAQAAKLKADEKALAQRLQDEGKWRHLW
                     RIAGHYANYSVFDLSSVEELHDTLMHLPLFPYMEIEVNGLCRHPSSIHQDDR"
     gene            44450..45379
                     /gene="catA"
                     /locus_tag="VFF22_13395"
     CDS             44450..45379
                     /gene="catA"
                     /locus_tag="VFF22_13395"
                     /EC_number="1.13.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019615 - catechol catabolic process,
                     ortho-cleavage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 1,2-dioxygenase"
                     /protein_id="HZX17841.1"
                     /translation="MSIKLSHTESVQSFFKEVSGLNNDQGSPRMKALVNRILMDSIRL
                     IEDMQVTTEEFWKAVDYLNRMGARSEAGLLVAGLGLEHYLDLLLDAQDEAAGLLGGTP
                     RTIEGPLYVAGAPLSEGEARMDDGTDPGTPMYLHGQVLDTDGKPVAGAVVDLWHANTK
                     GTYSYFDSSQSEFNLRRRIVTDAEGRYRARSIVPSGYGCPPDGTTQEVLNLLGRHGNR
                     PAHIHFFISAPGYRHLTTQINLAGDEYLWDDFAYATRDGLIGDVTFIEDAAAANGHGF
                     QGARFAEVNFDFQLQGAALSDAEQRNPRPRALQ"
     gene            45596..46162
                     /locus_tag="VFF22_13400"
     CDS             45596..46162
                     /locus_tag="VFF22_13400"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZX17842.1"
                     /translation="MLKIGLVYFSNTDVTGELMGAINDQLLGLGCSVIKHRISGSEII
                     NGRFERPALFQELHSCHAIIFGSPTYMGGVSAQFKTFADATSDFWESQTWAGKLAAGV
                     TSGTGLNGDQGHTLSYMQLLASQHGMLWVSLDAPFNDAEKGVNRLGCHMGVTAHSTTG
                     KVCELDIKTANYLANKIVGYAIRFSAET"
     gene            47048..47497
                     /locus_tag="VFF22_13405"
                     /pseudo
     CDS             47048..47497
                     /locus_tag="VFF22_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003192413.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            48214..49665
                     /locus_tag="VFF22_13410"
     CDS             48214..49665
                     /locus_tag="VFF22_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZX17843.1"
                     /translation="MSVPMNPAVSSNELRRGALSVGFIIFFVVSAASPLSVLAGGFPI
                     GIMLGNGAGTPVLLGLTLLVLLAFSVGYTTMARHVTNAGGFYAFSARGLGGLAGGAAG
                     VLAMFAYNILQVGLYGMFGGVAAGTMASVFAIDLPWWVYSLLAMASIAILGYRNIDLS
                     ARVLSFIVVAEYLAILTLDIAILKSGGDSGINLDSFKPEHVFSGNPSIGLLFCFAAFI
                     GFEATTIYGEEAKDPQRAIPFATYASVLLIGCFYGLSIWAMVVGVGSDKIVPLLQSLD
                     DPTTFIYGLSDHFVGPELTQLIRVLFLISIYAGLLAFHNSAARYFYATGRDGLLHGLL
                     GTTHRIHQSPHMGSALQTLIAAVVVLIFAAAGADPILQLFAWFSSLAALSLILLMALT
                     SIAVLAYFRKHPELGLSLTRRFILPAFSGIALICVLVLAVIHFEVLTGSSKLLSYGLC
                     ILIPISLFVGVMLAARLRRVSPERFQALGSHKL"
     gene            49747..51171
                     /locus_tag="VFF22_13415"
     CDS             49747..51171
                     /locus_tag="VFF22_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003200786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZX17844.1"
                     /translation="MNDYQMLIDGAQVAGEGEPFEVINPATGQAFALCPAGSVAQLEQ
                     AVEAAQRAFRSWGRTSCVERCERLTRIADDIEREAEVLARLIVMEQGKPLALAFSEVM
                     GGVAWTRYAASQELPVELIEETSTQRIELHRKPLGVVASITPWNWPFMIAIWHIMPAL
                     RAGNCVISKPSSLTPLSTIRLVDIIARHVPAGVINIVTGERGFGSAIASHPRIDKIVF
                     TGSTETGQRVMQAAAGNLKRLTLELGGNDAAIILPGTSVEAVAEDIFQAAFLNMGQTC
                     AALKRLYVHESQYQAFAEALAAIARRQVVGDGMEVGVTFGPVQNPEQLQLVAELVEDA
                     CAKGGQALCGGAPLPRAGFFYPPTIVTGVSDGHRLVDEEQFGPVLPLIAYRDVEDALR
                     RANASTMGLGGSVWGPDTAEAQAVAARLESGVAWVNCHAQIQPNTPFGGCKMSGFGVE
                     FGQEGLLEFTTQQLLFVRKPTAPQ"
     gene            51243..52589
                     /locus_tag="VFF22_13420"
     CDS             51243..52589
                     /locus_tag="VFF22_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014337967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZX17845.1"
                     /translation="MYAKYKTAEKKFWHPMSASAPANRANTLIIARGDGNYITDIDGN
                     RLLDGVGGLWNVNVGHNRSEVKAAIAAQLDELAYYQTFDGIAHPRVFDLAERLTSMFA
                     QEKMARVLFNSGGSDAVETALKMARQYWIASGQPGRTRFLSLRNGYHGVHIGGTSIGG
                     NGVYHYNHGQLLAGCHLLDTPWLYRNPWDCRDQEELVEHCIRQLEDQIAFLGAGTIAA
                     FIAEPVQGAGGVIVPPSAYWPRLREVCDRHDILLIADEVVTGFGRSGCMLGSRGWGVA
                     PDILCLAKGISAGYIPLGATVFNQRIVDAIENAPGFANVIMHGYTYSGHPTACAAALA
                     VLDIVEKEDLPGNAAKVGAHLIDGLTPLSERFDVVGEVRGKGLMIALDLVADKRTRQP
                     LDPASGQASRIAEAARAQGVLVRPVGNKIIISPPLTLTTGEADLIVDVLHNALTKH"
     gene            52676..54001
                     /locus_tag="VFF22_13425"
     CDS             52676..54001
                     /locus_tag="VFF22_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011534267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17846.1"
                     /translation="MKMNARHNSKIALALAVTAALSGQTQAYELYADEDTQLNADMLA
                     VFGWMNSRKNYDGTPGGSSWREGFIKYGLSGQQGLGEAGSLYGAFNLVSSGTWGDGDA
                     GGNSVGSERTTKIEDAYLGWRSADLFPVLGKDGLDVSFGRQVVQLGKGFLINDDGPNL
                     GKGPADGALNRGGAYYLAARHAFDRTAVVRIGGQDGLHSTLAWLKSDNRAQAETELTA
                     ATLDYRGEAGLLGLTWIHGLDVSDEWADDFLQQRDGMDIYSLRGESTAGIENASFAFE
                     YAWQNKDAGDEKAWYAEAGYTFADAPWSPSLTYRYTRYSESWDALFTGLNSPELGTWF
                     QGEVAANYAGLFARNNDVHHLAFKVRPLENLTLGALFFDFRTLHERDTVNRDARELDL
                     YAEWVVNDHLIITPLVGLFKPDRSDSNGGTQFGGNGTNVYSQLVVAVPF"
     gene            complement(54090..55154)
                     /locus_tag="VFF22_13430"
     CDS             complement(54090..55154)
                     /locus_tag="VFF22_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX17847.1"
                     /translation="MKPTPGLRRQIIHSLAVMALGIICLSVIGTYVFYAFAVTYLPGS
                     ISDTMVPSQLELVWIVCTIIAALSMAVFVAIRLSRRLITPLNSVAHSLREVAQGKLDA
                     RAPLDAQAMGETAQLVLDFNTMAERLQSMTREREFWNAAIAHELRTPVTILRGRLQGL
                     AEGVFSPSSELFEGLLRQVEGLNHLIEDLRVLSLNDSGHLQVQRSSVRLANELGTVLQ
                     AFATPLAEKGFHLHQELDAELLVECDAVRIRQALMALLENAQRHADPGNLRVCLEQVG
                     GDCQLSVADDGPGIDSALTADIFEAFRRGDPSRSRKSGGSGLGLAVVKAIAEAHGGQA
                     YCQPSANGGSRFVVSWPLQP"
     gene            complement(55151..55885)
                     /locus_tag="VFF22_13435"
     CDS             complement(55151..55885)
                     /locus_tag="VFF22_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX17848.1"
                     /translation="MSDSYARTSPASPATALVLIAEDEAEIADILIAYLQRSGLRTQH
                     ASDGQQALALHQTLKPDLLLLDVQMPVFDGWKVLSEIRSRGDTPVIMLTALDQDIDKL
                     MGLRLGADDYVVKPFNPAEVVARVQAVLRRTRGSAPAATQVLRVGAFQIDLDSHEASV
                     QRDGQQHTLELTLTEFKLLACLMRAPKRAFSRAELLENCLPEGDTQERTVDSHVSKLR
                     KKLEALDIQGVPTSVWGVGYRFGVES"
     gene            56047..57201
                     /locus_tag="VFF22_13440"
     CDS             56047..57201
                     /locus_tag="VFF22_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZX17849.1"
                     /translation="MGSKQRHLGIVLAALAVLGLAGCDAALEQAEATAPLQVSVLTLQ
                     AHELAVSEDLSARVVAVRSAEIRAQIGGIVQRRLFEQGAEIKAGAVLFQINPAPFKAE
                     VDSAAAALQRAEAVLARARVQVERLTSLVQAEAVSRQVYDDAVALREQAVADVAQAKA
                     TLARRKLDLQFASVEAPIAGRIDQALVSEGALVSPTDATPMARIQQIDQVYVDVRQSA
                     SILDTLRQQAGSATPELDVEILGSDGKPLDMRGRILFSGIEVDAGTGDVLLRMLVDNP
                     QRRLLPGLYVQARIPRAHYPAALSVPQQAVTRSAGQASVWVVDAQGQVQAVPIEVGEL
                     VERRYRVVSGLSAGQQVVVEGIERLSPDTQLATRPWQPAVTSEQLSAVAR"
     gene            57218..60355
                     /locus_tag="VFF22_13445"
     CDS             57218..60355
                     /locus_tag="VFF22_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HZX17850.1"
                     /translation="MPQFFINRPVFAWVIALFIVLVGVIAIPQLPIARYPSVAPPSVT
                     IYATYPGATPQTLNDSVVSLVERELSGVKNLLYFESSVDTSGTAQITATFKPGTDAEL
                     AQVDVQNRIKAVEPRLPQAVRQNGLQVESAASGFLMMVGMTSPNGQFNEVALNDYLAR
                     NIVQELRRIDGVGRVQLFGAEQAMRVWVDPNKLTAYGLTMNELAQAIEQQNQSIAPGR
                     IGDEPALPGQRLTVPLTVQGQLSTPEQFAAIVLRAAADGAKVVLGDVARVELGAQSYG
                     FSNRENGVAATSAAIQLSSGANAVRTASAVRERLAELAPSMPAGMAYSVPFDTAPFVK
                     VSIKKVIYTLFEAMVLVFLVMFLFLQNLRYTLIPAIVAPIALLGTFAVMLLAGFSINS
                     LTMFGMVLAIGIIVDDAIVVVENVERLMATQGLSPKEATKQAMREITGAVIGITLVLT
                     AVFIPMAFGSGSVGVIYQQFTLSMAVSILFSAFLALTLTPALCATLLRPVSAEHHDKR
                     GFFGAFNRGFERLTASYGARVARLVGRSGRMMAAFAVLCAVLAVAAAQLPSSFLPEED
                     QGYFMTSIQLPTGATSERTLDVVKAYEAHVATRPGLDANLVVLGFSFAGSGPNAAMAF
                     TMLKDWDQRNGATAAEEAALAQQAMADNVEGTVMSLMPPAIDELGTSSGFTLFLQDRA
                     NRGEAALLAAQAQLLGLAAQSEVVSDVYPDGLPPGESIRLEIDRQKAEAMGLSFAAVS
                     STLSAAMGSLYVNDFPNAGRMQQVILQADAPARMQLDDVLGLRVRNAGGGMVALREVV
                     TPVWSESPQQMMRFQGFPAVRLAGGAAPGVSSGAAMAEMERLVTQLPQGFAVAWTGQS
                     LQERQSAAQAPILMLLSALVVFLVLAALYESWSIPLSVMLVVPLGLLGAVAAVMLRGM
                     PNDVFFKVGMITIIGLSAKNAILIVEFARQLHAEGRTLVDAAVTAARLRLRPILMTSL
                     AFTLGVVPLMLASGASAETQHAIGTGVFGGMLSGTLLAVFFVPAFFVFVIGTQERFSA
                     WRAQRGATASPQENP"
     gene            60355..61791
                     /locus_tag="VFF22_13450"
     CDS             60355..61791
                     /locus_tag="VFF22_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZX17851.1"
                     /translation="MRLFLTLPLLACLSACSLTPTLERPAAPVPASFAMAATPPASTL
                     PASALGWRAMFADPRLQRLIDLALANNRDLRLATLNVEAAQAQANIQRAARLPAVGLE
                     GTRTRERALTTDANGQASREVSQQTGVNVALSAFELDLFGRVRALSDAAFARYLASEQ
                     GRDAAQIALVGAVAEAYFAQRLADEQLQLTERTLADWQQSLELARLLKQAEQSSSLDV
                     AQAEGQVAQAEADLEARRRALAQANNALQLLVGSELPGDLPVGLPLERQSLIAQLPAG
                     LPADLLLSRPDLRQAEQMLVAANADIGAARAAFFPQISLTASFGYASTSLAGLFDPAR
                     QIWHFAPQISQPLFQGGRLRAELRLAEVRKSEAVAQYERAIQTAFREVVDALAGTATF
                     DRQIKAQLRAVTATERRLQLSDLRYRAGFDGRLELLDAQRQLQAARQTLLELRRAEID
                     NRVALYKSLGGGLHARDIPEIAAVTAAQ"
     gene            61822..>63018
                     /locus_tag="VFF22_13455"
     CDS             61822..>63018
                     /locus_tag="VFF22_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX17852.1"
                     /translation="MCVVIPRRWLILLAVMLAFLPVVIDMTILHIAVPSLTLALGASA
                     TEVLWIIDIYPLLMAGLLVPMGTLADRIGNRRVLLIGLVIFGFASLLAAFAPTATLLI
                     GARVLLALGGSMIMPCVLGIIRRTFEDADERAMALGLWGTVGAAGAALGPLIGGALLE
                     HFWWGSAFLINVPIMLVVAPLVFLLLPRTEDTTPGTWALVQALLLIAGILAVVYGIKA
                     AVGTTQPLAVALSVAILGALLLTIFVRLQLRSATPMLDLSLFSRPAILAGIIMAAVAI
                     GALAGVELTLAQELQYVLGKTPLQAGIFMIPIMAAAAVGGPIAGYLSNQFGLRLVSSL
                     SLAVASAALAALAMTELHTPGWIVPTLLGFLGLTLSIGLTASSIAIMGSVDADKGGAA
                     GALEATA"
BASE COUNT        11576 a        18786 c        19981 g        12675 t
ORIGIN      
        1 gtgcggcatc agcaagaaca cagcttttct ctggcgccac cgcttcttgg cctgcgcggc
       61 ccagcaccat gcagcacatg aaacgggcat tgttgaggtc gatgagacgt tttttctgga
      121 gtccttcaag gggcagcgca agctgccaag agccccccgc aaaaggggcg gcgtgggcaa
      181 ggatcagatt cctgtcatgg tggtgcgtga tcgcgagggg cataccgcag acttcaagct
      241 ggaaaagctc gatgcagagc atgtgcgagc agccttactg ccgctgatcg accgggaagc
      301 cgtactgtgc agtgatggcg ctgcggtcta cgcaaacttt gccaggaccc agggcattac
      361 tcatcatgta gttcactcgc gtcctggcga gcgggtaagg cagggcgctt tccacattca
      421 gaacgttaat gcctaccaca gccgcctaaa gagttggatg actcgctttc atggtgtccc
      481 cacccgctac ctcccgaatt acctgggctg gcgccgcatg cttgagcgtt atcagcagcg
      541 tattcagcag gttcactgta tccaggaggc gattgggaga accatgcaac acgctattgg
      601 gacatagcca taaaaaagcc cgcacgggag gcgggcaagg gagcaaggcg gataatagcg
      661 tttagatgtc gagtaccagc ttggctgact tagagcgtga gcagcacacc aggattgcat
      721 cgttggcggc cttttcgtct tcagtgaagt aaacgtcgcg gtgatccggc tcgccttcta
      781 tgacgtcaca caggcatgtg ccacaaacac cctgttcaca ggatatctcg atgtcgatgc
      841 cgacccccgc aagggcctga aggatgctct ggccctcctc cacggtcact gtctttccgc
      901 ttcgagctgc caccacctcg aaactggcac cgctgttgtc aacgtcggct tgaaagtact
      961 cgcgatgtat ctgcgtgtct gggtagcccg cagtcaaggc cgtgttaatc acccaatcca
     1021 tgaaaccact tgggccacag gtatagaggt gtgtaccttc ctgtgcctgc cccagtactg
     1081 tctcgaggtt cagcttgctc tctggctcat cgtcgaagtg cgtaaaaaca acgtcagcga
     1141 agtcggaacg ttccagttca tctaggaagg cgctgcggct gcgagaacgc ccgcaatagt
     1201 gcagttcgaa tgtttttccc tgtgctttaa gtgcatacgc catggccagc aaaggggtaa
     1261 caccgatgcc tccgccaatc aggattgacc gatgggctgt taagtccaag gggaagtgat
     1321 tgcggggata gctaatgtcg actgccacgc cttcatgcaa ggcatcgtga acgccaaccg
     1381 aaccacctcg ggactgagga tctttcaaaa cccccagccg atagatggct gcgttggctg
     1441 ggtcgccaca cagcgagtac tgccgggtat gtcctgaggg caaatgtaca tctacgtgag
     1501 cacctgcctc gaacgcagga agatttcctc cgttcttgag caagaggtca atgacgacta
     1561 cgccatcgcc ttgcagctcg cgtttgtgca ctacaacatt aagaagctca ttcattggtg
     1621 cgtacctgta cacccccaag cagcataaaa aaatctactt gggggttgta atgaaaaaac
     1681 aagttacggc ttgcgatgat ggttagggtc tatgcccgtt tcggcgcggt cgcgaacgaa
     1741 gcgggaacag atcctagtct caaaaggtac gcatgactcc agctttcagg atggtgtgat
     1801 caagcaggcc ttcttcgccc agttcgtggc cgaaaccgga gtccttcaca ccaccgaatg
     1861 gcatgctgag atccaaaaca ttatggttat tgatccagac cgtacctact ttcatgcggc
     1921 tgaaaaatgt attgatccga tcaacatcct ttgtccagat actcgcgccc aaaccaaatg
     1981 aggtggcgtt ggcgcgctgg ataacttcaa gatcatcctt gtaagagaat gcggtcagca
     2041 ctgggccaaa aatttcccgt tgaacgatct ggtcgtcgtg gcccgccccc gtcagcacgg
     2101 taggttgaat ataggcccct gggccgctga tcgcttcgcc gccacagagc acttcggcac
     2161 cagactcaag tccctgcttg atcatcccca agaccttgtc gcgatgcgca ttgtgtacaa
     2221 gtgggcccat ctgcgaggac aagtccaggc ctggacccac tttgatggag cgcgcgatct
     2281 cggcgacacc cgccaggact tcggcatagt gacgctcgtg cacgaacagg cgagagccgg
     2341 cgcagcagtt ttgcccgtgg ttaccgaaaa tagcccacgc ggcgccggga atcgcgatgt
     2401 tcaagtcggc atcctcagcg atgactactg gagatttccc tcccagctcg agcgtgtatt
     2461 ttttcaacag cggtgcggct actgcagcga tacgcttgcc tgtttcggtc gagcctgtga
     2521 agctgatctt gttaacgcca gggtgctcga ccagcgcggc gccggcttca tggccaaggc
     2581 cggttacaac gttgacgacg cctggcggaa tgccggcctc attgcacagt tgcgccaggc
     2641 gcagtgcggt gaaaggtgtt tgctcagccg gtttcaggac aatcgtgcaa cccgttgcaa
     2701 ggaccggggc gagtttccaa atggcttggc aaagcggcac gttccacggc gtgattgccg
     2761 ctactacgcc taatggcgcg cgttttgtga aaccagaaaa ttgctggtct ggtaggtagg
     2821 gcaccgagag atcgaaggtt ttgcccgaaa tcttggtaga ccagcccgcc atgtagcgca
     2881 gtgtataaac tgaaaaatct acgtcgaatg cgcgggtatt gcggatcagg cggccgctgc
     2941 tcagcgtttc gagttcagcc agctcctgag cattttcagt tagcagatca gcgagccgga
     3001 gcatcagcgc ttcgcgctga taaggcttca ttttcgacca ggcactgtct tcgaatgctt
     3061 tacgtgctga agagacagcg aagtcgacat cgtgcttgtc cgcctgagca atctcggcaa
     3121 gctcaagccc gttactgggg tcaacgatag ggtaatactg ccctgaaagg ggattcctga
     3181 actcaccatt gatgaacagc tgagtacgct tgccgattgc ggattgagcg ccggcggaaa
     3241 ggttgtcgta gttgtttgcc aatgccatga gcgaatgcct attgttgagg ttcgggtgga
     3301 ccgtttcttg tatttagtcc ctactgggca cgtctaactg cgttgtcatc gctgcgttaa
     3361 aaacaggctc gggcgcgagt ccgatcaaaa tgctgattta cagctcgtaa acgccgcttt
     3421 tcgccttttt tgccttgcgc tgactgcccg cttacgtttt tagaggtgcc ctactgaggt
     3481 taatttctct cagctgtggg gtggccacaa tgacatcaat cgtggagttt gttgtcttta
     3541 ctgcaacaag ccgtttttgc ggtttcaaga ggtcgcattt ccagtccata aggcgctgtg
     3601 aaatcggcga tctgcaaggg cagttggcgc attttgggcc ttatgctggc gaccggcttc
     3661 tccgaccatg gcattggttc cggcccggcc gttggctgcc gatctgctct gcgctgtggc
     3721 gccgattatc gcgccttctc cctttcgatt cgttcggtac cgagggtgct acgtggtggc
     3781 ttgcgcaagg aaaaaacatc agcgctatgc gcacaggtca ggggcattcg caggcagtgg
     3841 ataggccttg gaaaggctct gtccctttgc gctctccttt tacccgcgct gccacaactc
     3901 cagattgact gacgcaagcg ctctcaatcg ctccccagaa aaactgcctt catgtcccgc
     3961 atgcacagcc tgcacgggaa gctcgcacag tcgctgcagc gagcgaatgt agtgggcgcg
     4021 gtctccgcca gggatttcat cgagcaagtc gccgtcgtag atcacatcgc cagaaaacag
     4081 aatgccggtg tgcttttcga acaacgcgat gctgccgggt gaatggccag gcaagtgcag
     4141 gatttcaaag tgccgatccc ccagatcgag catgtcgcca tccgcgagca aggcggtagg
     4201 cggcgccggt tgcaaacgcc aggcaagggg gtcaaaccct tcgtagggca gggcatcaat
     4261 caccaggcgt gagtcgtcca ggtcaggcat atacagcgcc agccaatggt cgggttgcag
     4321 gttcaggcga tcgctctggc tcgggttggc gaggagtgcg gcttccgctg catggcacaa
     4381 gcggcagtcg aattcgtgca ggccgcccat atggtcgagg tggctgtggg tggcgatcgc
     4441 ggccagcggt ttttcgaaca ggtcgcaagc agcgtcacgc aggctggcaa cgcccattcc
     4501 ggtgtcgatc agcaggtcgc gcgtgcgccc gcgaacatgc cagatattgc agcgcagcat
     4561 ggggtgcaca tggggttccc acagcagcgt gatgccatcg gaaaaggttt tgcgctcgaa
     4621 ccagcgctgc gcgattttca gcgtcatggc ctattcctgg ctcacttggc tacggaatct
     4681 ggcccactcc cgcgtgcgtt gacgcagggt tttagcgctg aggggttcct caccgaagag
     4741 ggtctggcgc acgttgctgg gggggaacag ggtggtgttc tcgcgcagcg tctgggttat
     4801 ttgcgccacc gatgtcgagt tggcattggc gtaaaaaatt gcattggtca attcggccat
     4861 ggcggtggcg ctggtgagga attcgatcag tgctttggcc tcctgcgggt ggggcgcgtc
     4921 cgccgggata gccagtgtgt cgatccaggc cagcgtgcct tcacgcggaa tacggtactc
     4981 gacggtgtgt gcctggcccg cttcgatagc ttgggcattg gcggtcacgg cgtcgccgtt
     5041 gtaggtcagg gccaggcaca ggttgccgtt ggccaggtcg ctggcctgtt tcccgaaggc
     5101 gatataacgc aggtttggcc gcagctcgtt caacagttgg ccggcttgcg ccagttctgc
     5161 gctgctgtcg ccgtaggggt tcttgcccag gtagtgcagg gcgatgctca gtacttcctg
     5221 aggcgagtcg ataatcgcta taccgcagtc tttcagtttg ctggcgtact cgggcttgaa
     5281 tagcagatcg aagctgttca gcgggggatt gggaatccgc tgttcaatgg cttcgcgaat
     5341 aaacgccagc ccggtcgtgc cccatgcgta gggcacagcg tagcggtttc ctgggtccac
     5401 cgcggcgagc tggctgagca attgcgggtc gtattcagcg gcctgcgggt agtgctgcat
     5461 ctgcgacggc tgcaaggcat cggcgcggat caggcgatcg agcatggcca caccgggcat
     5521 caccacgtca tagccgctac ggccggtcag cagtttggcc tcgagcatgt cggcgctgtc
     5581 gaaggtgtca tagtggatgc ggatgccagt ttgctgctcg aagcgcacaa gggtttgcgg
     5641 ggctatgtaa tcagcccagc cgtagacgtt gacgctgcgc tgaagcgcag tctcggcggc
     5701 gccagcccca cccgagatca gggccagggt catgaccatg agaagccgtt tggaaacagg
     5761 tgagccgcgc gagtgagcca tgggtaaatg acctgtaggg agacatgcgt tgattatttt
     5821 ccggatggca aaaaacagac aaactatata atttgtcatt ttctaattta gtagagctaa
     5881 acagttgatg ggccttcctc ctctcaatag cctgcgtgtg ttcgagaccg tggcgcgcct
     5941 tggtcatctg ggtgcggcgg cggcagagct gcatgtaacg cccggtgccg tcagtcagca
     6001 aatccgtgcg ctgcagatca acctgggtgt cgagttattc cacaaacaag ggcgtcactt
     6061 ggtgcttacg ccatcgggtc tggtgctaca gcgcgcagtt gcccgaggta ttgaggaaat
     6121 cagccagggt gtgcgccaag tgctcaccga ggtgcaggtg gcggcgcccg acacggtgct
     6181 gaccatatcc actgagccgg tgctgggtac gtcctggttg ctgcccaaac tgtttgcctt
     6241 ccgcgcgcaa catcccaata tccacctgcg agtgatcacc gccagcacgg cctcggaggt
     6301 cgactggaaa aaagccgaca tcgccctgtt gtacgccagc ccgccgtggg agggcctttg
     6361 gtggcggccg ctgcgccgcc tgcatatgtt tccggtttgc tgtccgcaac tgctgccaca
     6421 ggttcgccag ccggccgact tgactaacca acgcctgctg cacgaagacg atggtgcgct
     6481 ctggcgccgt tggttggtgg cggcgcgcgt gccatatccg ggtgatgccg acgtatttat
     6541 ccagagcttc ccgatggcgt tgcaggcggc acgcgatggc tacggagtgg cgctgagcga
     6601 cgaattcaac tcccaccgcg accttgccga aggccgcctg gtgcgcccgt ttacgctgca
     6661 agtgccaggc gggctggact actactgcct cagcctggaa gagcgacgtg agctgccgga
     6721 ggtggttcag ttgattgact ggctgattga agaggcgggg cgggatgttt gtctgtaaat
     6781 aaggggcgcg gtggatttag atgactttca atcttttctg ctggcccgta aataccgctt
     6841 catggaggac cgcattcggc ccaaaggcag accattgaga atgggccgct cggatgcagc
     6901 ggtgctcagc ccccatacaa tcggggcaag gcaatcatcc acccagatcc cggctggccc
     6961 gcccaggttt ttcaaccgcc agctaagtcc atatcaacca tctggtcgat aggcgcgcgg
     7021 tgccagggcg agcaccatcc aagcaccaca caccacccga gatatcccca tgagcacgaa
     7081 atccgattgg atcaccgtag gtgccctggc cgatggcttt gccccggctg cctttatcct
     7141 gccgaacctg gccgacctcg ccggcaaaac cttcaccctg aatttcgcca acggctggca
     7201 gatcacccat gcattcgagg ccgagcgcct gagctgggta gccgccgatg gccactcgca
     7261 aggcagtgcc acttatcgcg ctacttcggt acgccccggc atctacctgg tggacttcct
     7321 gaaaaacgaa aatggacagg actggtcgat cagcctggtg ctggatatcg acagcgcctc
     7381 cttcaccgcg gtgatcggcc gcctgcccgt cgagcagacc acccacgaag ggctttacca
     7441 ccgcgcactg gccggcaagc agctgaccgg tgtggaggta gagttcttcc acggcagcct
     7501 ggataagccg tggcaagagg gtgcctgccg ccatgcgctg acccacgaac tggtcggcct
     7561 gcgtaacctg tatcgctaca gcccgaccga ggtgtacgag cacatctacc tcaacgagaa
     7621 cttctatagc tggcagtgca tcaagggcgt ggaagccggt ctgtgcgaca ccgatcgctg
     7681 tcactattac aagatcgccg atgagctgta cctgttcgtc tggcgtgaga agatcgtacc
     7741 gaccctcggc ctggtgatga tcgacctggc taaccaccgc agcgatggca agatcttcgg
     7801 ttacgccggt ggcggtttcg acgagttgtc caacttcccg gtcgcctctt attgcagcgt
     7861 gttaaaccgc actgagtatc ccgatgagtg agcccaggac catcgtcatc accggggccg
     7921 gcacgggcat cggtgaagcc tgcgctcggc gcctggctgg cgaaggccac aacctggtgt
     7981 taatcggccg tcgtcgtcag ccgttggagc gggtcgctgc gctgaccggt ggcctggtat
     8041 tggtcggcga tgctgccagc agcgcgatct ggagcgggtt tgtcgacgag attcgtgccc
     8101 gcttcggcgg catcgatgca ctgctggcct gtgccggcgg ccatggtctg ggttcggcta
     8161 ccgagacctc cgacgaagtc tggcaggctg cgctgcgcag caatctggac agcgccttcc
     8221 acagtgctcg ggcctgccta ccgttgctgg tcgagcggcg cggcagcatc gtcttgttcg
     8281 gttccatcgc ctcgctggcc gccggcccac aggtgtgcgg ttataccact gccaagcacg
     8341 cgctgctcgg gctcaaccgc tccctggcgc gggactacgg cccactgggt gtgcgggtca
     8401 actgcgtctg cccgggctgg gtgcgcacac cgatggccga tgaggaaatg cagccgttga
     8461 tggcgcattt cggcgaagac ctcgacgccg cctacgcccg ggtgaccgcc gatgtaccgc
     8521 tgcgtcgccc ggccagcgcc gaggaaattg ccagtgtctg ccgtttcctg atcagcgtcg
     8581 aggcgtcgat catcaccggg gccagcatcg ttgccgatgg tggctccagc atcgtcgatg
     8641 taccgacatt ggtctacgac cgcctgggag gtgcctgatg ggcacacaat tcgatttcac
     8701 cggacaaacc gtgctggtca ctggcggtgc ccagggcatc ggccgaggta tcgtcgaggg
     8761 gttcgccgcc agtggtgcca gggtgttgat tgccgacctg cagatcgagc ccgcgcaagc
     8821 gctggcccgc gagctgcagg gcaacggctg cgaggcccag gcgctgggca tcgacctggc
     8881 cgatgaggta gccatcgccg ccttgctggg cgggctcgaa cgcctggaca ttctggtgca
     8941 caacgccggt tactttccac tgacaccctt cgccgagatc accccggcga ttctgcagcg
     9001 caccctggcg gtcaacctta gtgcgctttt ctggttgacc cagggtgcgc tgccgctgtt
     9061 cgagcgtcag ggtaggggct gcgtgctggt cacttcctcg gtcaccggac cgcgggtggc
     9121 ctacccgggg ctgagtcact atgcggcgtc caaggccggg gtcaatggct ttatccgtag
     9181 cgccgcgctg gagctggcca agtgcaaggt gcgggtcaac ggcgtcgagc cgggcatgat
     9241 ccgcacgccg gccatgggca atctgggcga tggtgaccac agcgccgaca tcgcccgcgg
     9301 tgtgcccctg ggccgtcttg gcgagcccga ggatatcgct ggtgccatgc tgtttctcgc
     9361 ttcggagcag gccagctata tcaccggcca gaccatcatc gtcgacggcg gtgccacgct
     9421 accggaaacc caggcggcgc tggactgacc tcgtagggtg cgccgtgcgc accagaaatt
     9481 tgcgctggtg ttggtgcgca cggcgcaccc tacaggcagt aagtacataa ccttttcaat
     9541 gcgccgaacc gagctccatg aagactcctg cacggtagtc cttgggcgac tgctcgaact
     9601 gcttcttgaa ggcacggctg aagtgcgccg aatcggtaaa gccccacttg taggcgatgg
     9661 cagtgatcga ttcgcgtcgc agatgtgggc tggacaggtc gctggcgctg cgctgcagac
     9721 gggtgcgctg gatgtaacgg cagacgctgt caccctgttc ttcgaatagc cggtagagct
     9781 ggcgcacgga aatgtgcagg cggttggcca gggccgcggg gttcaggttg ggctgggtca
     9841 acgattcttc gatcagctgt tgggcctggc ggcgcaggct gttgcctgcg atcgagtcgg
     9901 ctatttcggc ctggttttca cgctgcacca gcgccgggac gagcaggctg gtaaaggccg
     9961 acagcatgga ctcggtctcg ttgtcgtcgg tgtgtgtgcc ttcttcattc atatacagct
    10021 ggtcgaccag caggcgcagc atgcgaccgc tgctgcaatg ggcggaagcc ttgccgaata
    10081 gctggcgatc gcactggaag gacttcagca cctgctcgcg gggcagatgc agcgaggcat
    10141 gctcaatcag gccgaggggg gtgatctcgc atgagcccac agagtccatc aacaccaggt
    10201 cgccgggggt aagctccaga gtcacgccgt tctgggttat tcgcgaacag ccgctgcgct
    10261 ggctgaccag aaagcaatag cgatcgtcat cccgatctga ctttgcattc ctggagatca
    10321 ggcccgcgtt ggtcttcagg tgcgccaggg tcagaccggc gtgttcttta gcgcagacat
    10381 cgccgataaa cagcgcattg ttgaaggcca gccgggtttc atagttaccg caaaccgact
    10441 gcattgagcg ttgccagttc tcgagggcgc tgtgcgtgac gtgctgtgcg tgcatggcaa
    10501 tctccgaatt tcgttaacat gttacttagt gaagcaaaat agctttcgtt aatatgttaa
    10561 gcatgttccg tgccgctgcc tcgcaggttg gcccgtgaga acatttgcgg ctgtctggat
    10621 cgggctttca ggcagattta tcaggtctgg gaggacgtgc ctagcgcatt tattcggtgc
    10681 gcaatctgtt acccctgcat cgatttcacg cactgacatt tgctggcgcg ctggcgcagt
    10741 cgatcaaggg cgttggcaag tgcggacaag cattgcctgc ctcaggtcgt ccctaattag
    10801 ctcgtcatcg ttagccgtgg ccggagatcc cggggaggga gcaggagccg gtagcggtgg
    10861 cagcgtcccg tatggcgggg cggatacctc gatggccgtc ttgtcctatg gtctggtcag
    10921 aacaacaagg ggaacatccc atgcaccaag aacgtcgttg tctatccatt tccaaagccg
    10981 tgtgcctggc cgtcgcagcc cttgctgccg gtcagtcaca agcttacgag atctacaacc
    11041 agggcgagac gaagctgaac gcggacttcc tcgcggtata tggctggttc aacagccgca
    11101 agagttatga cggcagcagc ggcggtgcca gttggcgcga aggcttcgcc aagtacggga
    11161 tcagcgccga gcagggcctg gcgggtaatg gcacggccta cggcgcgctc aactgggtca
    11221 gctccggtac ctggggtgat ggcgacgctg ctggtctcag cgatggctcc gagcgcacca
    11281 ccaaaataga ggatgcctac ctgggctggc gttccggcaa tctgctgcct gcgttgggcg
    11341 agaatggtct ggatatctcc ttcggccgcc aggtagtgac cattggcgat ggctttatca
    11401 tcaacgacga cggcctgaat ttcggcaagg gtgtagcaga cggcgaattg aaccgcggtg
    11461 gtggctacta cctggcggcg cggcgcgctt tcgaccagac cgcggtggtg cgcatcggcg
    11521 gcgaggaggg gctgcatggc gacctgatgt ggctgaagtc agataaccgt gcccaggcca
    11581 aaaccgaact ggccgctacc acgctggcct acactgccga accgggcacc ctggccatga
    11641 cctatatcca tggcattgat gtggatgacc aatacgctag cgaatcccag cagcagcgcg
    11701 atggcatgga tatctacagc ctgcgcggag cgggtaacgc cggcgtcgag aatgccaact
    11761 tcgccttcga atacgcctgg caggacaagg acgcgggaga tgaaaaggcc tggtacgtcg
    11821 aggcggggta cacctttgcc gatgtggcct ggtcacccaa cctgacctac cgttacagcc
    11881 gatactcgga aggctgggat tcgatgttca acggcttcaa tcgcggctac ggcacctggt
    11941 tccagggtga ggtggcagcc aactatgcgg gcccgttcaa cagcaatacc cggatccatc
    12001 atgtcggctt caaggtcaac ccgctggaga ccctgaccct gggggcgctg tacttcgact
    12061 tcgatacgct caatacccgc gacaacccga acatgggcgg tcgcgagctg gatctgtatg
    12121 ccgagtgggt ggtcactgac aacctgatca tcagcccgct gatcggcttc tatcagcccg
    12181 acaagagcct ggaaaacggt ggcctgcagg gcggcggtaa cggcactaac gtctacagcc
    12241 aactgacggt ggccgtaccc ttctaaaacc ttcgccccgg cagccggtta aacacggctg
    12301 ccgggagcgc ggtgcttaca ctttggcgct gcactgctgc gttatttttg agtatgccga
    12361 gattttgctc agagggtgat aatccgacgt attgagtcgc ccagatacct taatcattgc
    12421 gacccttgat taggctgtgt caaaagactg catgggataa ccttgctcca catttcaact
    12481 gaagctgcta actaaatgat gggttttgaa aaactagctg agttacgcga ccgcctgcgg
    12541 gccgaaaaag caggaaaaac ctgagactac ttcagaccat ccctaaaggg ataactacgt
    12601 tcttcaagac actctttagg ctcagaaaat agcgcgtttc aaactctatc ggcgatagtt
    12661 tgtagagttt gagtggcgac atttaataag ccagataagc ctttaaggtt cggcgtgatc
    12721 taatttattt gtcaggtatg gaactgttgc attagctctt tctggtaatt ggcgagctca
    12781 tttagcttgc ggctaacttg agcagattgc tcagcctgac ccgataagga gtctgtcacg
    12841 ttccgtattg cctggatatt gcggttaatt tcttcagcga cacagctctg ttcttcagca
    12901 gcacttgcaa tttgtaggtt catctccgta attaggttta ctgctttgtc tatacgttga
    12961 agggcggcta cagcctgctg aacttggcta acattctgat gtgctgaatt atgactgact
    13021 tgtatggttt tgcttacttc taatgttcca ctctgtagat tttcgatcac aaggcggatt
    13081 ttctccactg actcttgtgt gtgtttggct aagttacgta cctcatcagc aaccactgca
    13141 aaaccgcgac ctgcatcacc tgctcgtgcg gcttcgattg ctgcgtttag ggccagcaga
    13201 ttggtctgtc ctgcaattga gagaatgacc tccaggactg caccaacctg ctcgctactc
    13261 tgagccagac cttgaagctg taccatggct tcattcaggt cccgtgcctg ggcatcaatt
    13321 gcttgggtcg tctgggtaat cacggttaaa ccttcacgac ttgctaagtc agcgtgacgg
    13381 gcggcttcgg cggcttgggc cgcgctctga gctgacgagt tagcggttgc agacatctcg
    13441 tgcaacgctg tggctacttg gtcaatttcg cgaaactggt gctgcattcc ttcgcaggtt
    13501 tggcgtgcga tcatggctga ctggtctgcg gtatccctgg cattaagcac cgaggcctgg
    13561 atctttgcga tgactggttg cagtttgtcc aggaagccgt tgaacgccaa agccaggcga
    13621 ccgagttcgt cgctacgagc atagtccagg cgacgtgtaa gatcgccttc gccgtctgcg
    13681 atgtcctcca gcatagccgc gaggtgaagc agcgggcgtg ttacacccag tgcggcaagc
    13741 catatcatta caataccgag cagggccgcg gccaggccga ggccgagctc taaggctgag
    13801 ctctgcaagc gctcttcatc cattcgtgcc gtcaccagct ccgcgggagc cagtagtacc
    13861 tgttcgggta cgctgagcag gattccccaa ggcgcggtgc cggctatagg cgttagcggt
    13921 tccagcatgc ttatttggcc gctatcagcg aaagcgtaat ttttttgctg agtgagtgcg
    13981 tccttgattt cggttgcatg aactgtcagt acctgagaca gcggcttacc cagttgcgcc
    14041 gcttccgggc tatgggcagc tatcaagcct gaaggactaa tgatgctgat tgagccgtta
    14101 ccctgataga gttcttggct tgctgacact gcatctttct gcaggctatc taggctaatg
    14161 tccaagccga tgacagctat tactttgccg ttttccaata gtggaaatgc gatgctggtg
    14221 acgagtctac gagttcctga ggtgtcgtca aagtaaggct caagcagaga aagcgccccg
    14281 ctatcacggg ggcaggtgaa aaaagaatta aagggggctc cactcggccc tacagaggtg
    14341 tcctctagta gtttttcatc tccatataca gcctgaagct ctccggggtt tgcctgaagc
    14401 caatagagcg cgaagcgtcc agcgtcattg ctgcccaagt cattacgccc actaaattct
    14461 gtgtcttgcc cgtcaagggc gttaggttgg aaaatcagaa aaaggccaag tagttctggg
    14521 cgctgctcta cggccttgcg tagctcactc gcaagggttt cgcgaagttg gtttgccgtg
    14581 atctcaccgg ttttggcacg ctcacgtaga tagagtaagt gtctagcgag ccccagccca
    14641 aattcatggt tcgggtgaat ccctgctgta tgcgcagtgc ttgagcctta ccctgggact
    14701 cgaggtttag gcgggctgca tccgcgagca tctttgcgct cgagtctttg actcgctcgg
    14761 tgctagcacg ggtttgataa agagatagcc cgactaacaa tccgacgatc aaaaccagac
    14821 taaagctcgc cagaagagtg atcttgcgtt ggatactaaa gttgagcacg ggcgcatact
    14881 ccgataatga gcgtttccgc cccaggccgt tcatgcttat gtagtgttgg ccggttagat
    14941 ttgggaggct gccgaatcgc tatgtcgtga aaattcgcac cctgacatca taatcacgtt
    15001 actgcttatt attcgttcgg cggatcaatc ttcgctaatc atttgggttg gcaataaagc
    15061 cctcatatgg cttaaaaaat aaatagttcc aatagatttt tcttgtaggg ctctgccagt
    15121 gttttgtata tgcctgccat gcttcggtaa cggtgtgtgt ttagaagtgc gcggaattta
    15181 ttttggggtt aagtaattgc gaatagggca aaaaaagccg gcctagaggc cggcgaatgc
    15241 acacggagca aattggattt tgtataccag agcggttagt ccatgccgtc gctgatttct
    15301 atgccttgtt caaccatctt cttgatcaaa tcggcgcgaa cgtcggggag attaagcaag
    15361 gacaccttcc gagtggttgc cacttcttct tcggtatagg gccggtaatg ttcaggcgtg
    15421 ctaccaccgg ctatgccgtc cgtcttaagt agccagttga gcaagtcagc aagctgcgcg
    15481 ttgctcagcg ctgactgaga catgcctggt acgcgcacga ggaattcacg gccaccctct
    15541 accttgagga agttgcccac aaaccccttc atccgagggg tgtcgttggc cttggagcct
    15601 tcaccgtcgt ccagatggca gcccgcacac tgcaactgat agttgactgg tgtgctgtag
    15661 tgggcctgtg cgatgggttt ctgtggctcc tcgttgcccg gggcaggccg ctgatcggga
    15721 ttgggaatgg cgcgtgcgat ggccagcggg gcgaccatgg cgcagaccag tccgatgatg
    15781 agcttacgca tgatcgtccc cttggttcag actgtgacgc cgcggatgat ggcaacggtg
    15841 cagttgtaga tgtttgattt agctgccagg caccagttga tgtcgttgtt gttaaacggc
    15901 cggtaaagcg gctcctcgct gtcgttgcgg gtacaggcgc actgtgcgca gctgtgctta
    15961 ccgcagcagt cgttgtaggc aatgatgtag tccttgccat cgcctgggtt acggcaggtg
    16021 ccgatccagg tgacttggga cacttcggtg cctggcgggc aggaggtgac agaaccgccg
    16081 cagcaactgc acaggaagcc gtcgatggag cagtagcgcc agtagtcgca gctattgggg
    16141 tcgcctgggt caccggcttt gggcgcttcg gcggcaagtg ctttgctggt acggtcgata
    16201 ggcagcagca cgggcagggc ggcgccggcg accatcagtg agccgaggcg gccgaggaat
    16261 ttgcggcgcg aggtggtgtc ggccacatgg cgggtggagc gttcaaacag gcggtctagc
    16321 aatttcatgt cagggctccc tggctcagtg gttgtgcgcg ccgtgggcgt gctggctgtt
    16381 gtgcaggtat tgctgcaggg tggcgctgcc caggtgctcg gtctcgaaca ggctgtcgag
    16441 gtgctcgcgg gaattgacca ggcccttggc gcgcagtacg ccggcgttgt cgattagggc
    16501 ggcatagggc agcttgccga tctggtaggt catgcccact tccgggccga ccacgtaggt
    16561 ggcgtcgtcc agcttgtgtt cggtgatcag ggctttttgt gcgtccatat cgccgtcgct
    16621 gacgtagatc acgtcgaggc gatgggcctg ttctttggcg atcgatttga tggccggcag
    16681 cagcgatttg cagattgggc aggtgggcga gaggaagaac agcagctgag ctttctcacc
    16741 ggcatagccg aagttcaccg ggcgaccgct acggtcggca gcagtcactt gcggcgcggg
    16801 ctcgttgacg gccacgccct tatcgaccat aagcgcgccg gctggcgcca gacggccatg
    16861 caaaacgccg atctggcgaa ccaggcccat gacggcgaag gccagggcga tcagcaggcc
    16921 ccacagcaga atgttagaaa cgatcagagc gtccatgatt caccttccaa tgagtttgag
    16981 cagacggggg ctgttggcca tcaggccatc agcggcggca tagatgagca gagctacagc
    17041 actggcggcg atgacgacga acgcatcgaa caggcccagg ttgcggctta acggggcgag
    17101 actggcgacc agtgccaggc tcaccagaac ggtgttgcgc gccagcagca gcgggcggat
    17161 cggctgttcc tgctgtggcc cagcacaacc gcaatcgata tcgcggcggc ctcgccacag
    17221 gttgatgccg atagccaggg cgtagctggc gagcaacgcg gctgcgccca gcgcagcaaa
    17281 gtggcgagct gcgggcacca acagcgcaaa cgccaggcca gcctcgagca atggcagtaa
    17341 gcgggcgatc ggacttacca gagcctgcgg caggagctga taggcctcta gctggtttgc
    17401 gaagcgggcc ggcgccctca gcttgtgagt cgcggcgctg gccaggatca ccgccaccgc
    17461 gagtgctgcg gcaatgacga atatcggatc gggttgcatg gcggcacctc gtcagtggct
    17521 gagtatgtga atcgcggtct tggcgatgcg ttccacggtt ccggtgtgct tgccgctggt
    17581 caggtcgaag acctcgagtc cgccgaccac attggcgcct agcagtcgtg gcttgtcatc
    17641 ggtggtcgct tgcaggctcc agatcggcgc cggggcttcc agcgtgccga tacgcttacg
    17701 cgtttttaga tcgtagaccc agatgatggt gctcgggtct tcccatttca gtggttcgtg
    17761 agcgtcatgc atcagtgcat aaaggcggtt gagcttcggt gctacggcaa tcagttgcca
    17821 gccgcctgga gcccagccgg ctttcttatc ggcggcgtca ctgaccaact cccaggccgg
    17881 gatgatcttc ggctgctcgc cggagaaatc cacggcgcgc acggtgcccg tggtgctgac
    17941 gaagtagtag gtatcgccca cggcgtaggc gcgttctaca agcttttcgg cgttggggtc
    18001 gaagaaagcg gtgtgggtgc gggcgacttc cttgccagta tcgtccagtg tcaccacctg
    18061 cagattgccg tcgccgcaca gcgaggcaaa gctgcgtttg ccgaccgggt agttaagtac
    18121 gcagccggga atcgagatct cgctgatcac cttgcgcgcc tgggtgtcga ccaccgtgac
    18181 cgaggtggac ggggtgaagt tgtagatata gacgaagcgg tcgtccgagc tcgttcccag
    18241 cgcgtgacgc tcgggcagca tcactgcacg agtgctcggt atcacgacct cccagtccgg
    18301 agacagcgtg ctggtgttcc acgcggttag cacatcggtg cgagtaccac gggtgccacg
    18361 cgaatagaac aggtctgcgg tgaacagatg cttgccgtcg ttactcagtg tggacggtgc
    18421 cgcaaagccg gtgctgacca tgccgagcat gcgcttactg tctggatcga ccacagtaat
    18481 gcggccagtt accagattct caaactccgc atcgacgatg taggcacggt gcggtgacgg
    18541 cgggaatggc agggtttcct ggccgatggt gtcacgcggt agctcagcgt tggcgctcag
    18601 cccggccagg gacaggccca gcgccagggc agtctgccgg atgttccagg ggatttgcat
    18661 ggagttctcc ttgttcttgg acatgcaagg cattgccctt gaaggctgcc gcggtcgggc
    18721 tgatcggatg cgcaacatgc ggactgctcc aagggataat gatgatgaat gggcatcaca
    18781 acgatcctaa aaacgctgcc tgatggcttg taagcgcctg caagaaactt gtatttgcct
    18841 gccagtctcc atgcctgcca agcgcgctgc gtgtgatctg gcaggcatcg gcaaatgaat
    18901 ggcaggcaga tgaaagtcct tacagggggc agggcatagc ttcgagactc gtcaatccca
    18961 ctcatgacac taggggctac tatgtccaaa ttgccatcat ttcatctaac tacccttgct
    19021 ttgctgatcg gtactgcctt gccagccgct gcggtcgaac tctatgccga tgccgacacg
    19081 cgcctggagg gcaatcttga agccgcgttc ggtatgtttc acagccgaga gaactatgcg
    19141 gccagtggcc gcttacagga gggtagttcg tcctggcgcg agggctattt caagtatggt
    19201 ctgagttttg accaggggct gggggctgtc ggtagcagct acggcgcctt ctcgctgctg
    19261 agctctgcaa cctggggcga tggcgatgcc gccggctttt ccgatggctc ggaacgcacc
    19321 accaagatcg aagatgccta cctcggttgg cgttcgggcg aactgttcga ggcgctcggc
    19381 gagaacggcg tggatctctc cttcggtcgg cagaacgtta tggtcggcga cggtttcctg
    19441 atcaacggcg atgcgctgaa catcggcaat ggcttggctg atggcgaatt caatcgtggc
    19501 ggcgcctact acctggccgc gcgcaaggcg ttcgaccaga ccgcagtgtt gcgtttgggc
    19561 ggtgagcagg gctggcgcgg cgaccttatg tggttgaaat ccgataatcg tgcccaggcc
    19621 aagaccgagt tgtatgtcgg cactctggag catgtggccg atgtcggcac ggtggggctc
    19681 acctacatcc gcacccgtga cgtcgacgag cagtacgcct cgccgtcgca gctccagcgc
    19741 aaaggcatgg agacctacag cttgcgcgcg gcgggcaatg ctggcgtgga gagcctggcg
    19801 ctgtctggcg agtatgcacg gcaggatcgt gatcaaggca gcgacgagaa cgcctggtat
    19861 ctggaagcag gctggacctc cgccgatgtc gcttggtcgc cgactctcag ctatcgctac
    19921 agccgtttct ccgagggcta cgacccgttg ttctacggct tcagccgtgg cttcggtacc
    19981 tggttccagg gcgaggtcgc gggcaactac gccggcccgt tcaacagcaa cagcgcggta
    20041 catcatgtcg gtttacgcgc cagcccgctg gagaacctca gcgtcggcgt gttgtggttc
    20101 ggcttcgata cgctcgatcg cgagcgtggc gacttctccg gtcgtgaact gaatctctac
    20161 gccgagttgg ggctgaccga aaacctggtg gtgatgccgg tcgtggggct gtaccaaccg
    20221 aagaaaagcg ccgaggaagg tggtgctcag ttgggcaaca acgaccgtaa tctgtacagc
    20281 cagttggtct tcgcgttcaa cttctgaggc gtccccgagg gcctgaaaat cctctcaacg
    20341 caggccgcgc cgctatccag cggcgcgatg ccgcggtgca ataattagtg gatttatcta
    20401 ttatctggcg tcctctccag cgaagcggtt cattcatgaa ggtgccaaaa aagcgtcgca
    20461 atcgttacaa cccgatcagc gaagaatttc acaaggaaga gttcccgttc tattggctgg
    20521 tacgtgttca tgcgcgctac accatgagca tggagaggct gctgaagaag atcgatctcg
    20581 atatcccgcg ctggcgcgtg ctgaacattc tctacgaggc ccaggaagcg agtatttccg
    20641 atatttccga gttcgccgtc gccaagctct ccaccatcac caagatcgtt taccggatga
    20701 aggacgatgg cctggtcgat acccggcaga gcgaccagga tggccgtgtg acccaggtgt
    20761 ccatcaccga caaggggcgc gaagcttact tcgccatgca cgaagctacc gcacaggtct
    20821 ttcacgatgg cttcaggggc ttgaccgaag cacagatccg caagctcaac gagatcctgg
    20881 cgacgatgtt cgacaacctc tccgatagct gagcggcgtc gacctaaccc agcatgacct
    20941 gttgccgggt cgaggcatcg gctcgatagg tgcgcggaga aataccgtag tgctgtttga
    21001 acacccgaga gaagtgcgcc gagtcggtga atccccactt gtaggcgatg gtggtgatcg
    21061 gcagcgcatg gctgctggtg tcggcgagct cccgcgcact gcgttccagg cgctggcgct
    21121 ggatgtagcg acagatgctg tcaccatcga tctcgaactg ccgatagagc ttgcgcaccg
    21181 agacgttcat ttttgctgcc agttcggccg gcgtcggtgc gttgggcagc gactcgttga
    21241 tgatcttctc cgccaggcgc cgtagcggtc ggcccggatc cagcaggcgc tcgtcgttga
    21301 aagccggtgg caacagggcg accagggcgt tctgcagggc gctgccatag ggacgctgag
    21361 tggccgaatt gagttcgcca ttagcgatct gttgcaggat gccctgcagc agatgcccgg
    21421 tgggactttc ctgttccagc ttgccgaagc ggcgtgccga attaggtagc agcggatcga
    21481 ccatgtcacg gcacaggtgc accgaaagct ggcgaatcag cccttgcggc ttcatctcga
    21541 aagcctgcgc cgaatcgagc agggccatgt caccggggta gaggacgaat tcgcgatcac
    21601 cctggtcgat ggccatcacg ccttcgcgct gcatgaccag aaagtagtaa cgatcatcgg
    21661 cgcgcgccgt gttaccgcgt tgacggttga tggcctgagc gttggtgctg atgtcggcga
    21721 tctccaggcc gcccaggtcg atgcgctgga tttcgccgat gaaatcggca ccccatgtct
    21781 gttgagtctc gaaacgtcca caaacgctat ggacaccatg ggtccagcgc tcgaaagcat
    21841 gggaggaatg cacggtcatg aggcggcctc gctttattct tggcgttttg ggtgcgcttt
    21901 tgtttcgttt ctgcacactc tttaaataac gtgaatattc aagcattttt tgcgccaaaa
    21961 acggcgctgc cgagaccgtt ctgatgtttc ttatcagaca gctgcgggag agaggggcag
    22021 gaaacgggcg ggcggggctg acgacaggga ggctatgatc cggcaggaaa gacctgccgg
    22081 atactgagtg ctttagtagc ggatcattac cgacttcagt tcggtgtaat cgtcaatgaa
    22141 ggcgctgcca aattcgcggc cggtgcctga ggccttgacg ccgccgaagg gcaccgacgg
    22201 gtcgaggaag gtgtgcatgt tgacgtatac cgtgccggcc tcaatctgtg ggatcaggcg
    22261 aagcgcgcgg gacagatcgt tggtccagat gctggcggtg aggccgaagg gcgagtcgtt
    22321 gatcaggccc ggcagttctt cgacctcgtc gtagggcagc acgcagagga tcgggccgaa
    22381 ggtctcctcg tgcagcaggc ggtcgcgcgg gcccttggca agcaccacgg tcggctcgac
    22441 gaagtaaccg gggcgggcga gcggcttgcc accgcagacc acttggcttt cctcgtgggc
    22501 cagctgcagg aactgcgcga ccttgtcgaa ttgtggcttg ttggccagag ggccgaagtt
    22561 gacgctttcg tccagcggtg agccgatgtt catgccctgc agggccgtgc tgaacttctg
    22621 cagtgtttcg tcgaggcgcg agcggtgcac gtagatgcgc tccggtgcgg cacagacctg
    22681 gccctgatgc acgtagcccg tctggatcag accagccacc gcgctgtcga tatcgacatc
    22741 gggcagcagc gctgcggcgt tcttgccgcc cagttccagg gtcacgcggg tgaggttggc
    22801 ggccatggcg ctctggccga ccttgatgcc ggtgggcacc gaaccggtga aggacacctt
    22861 ggccacctgc gggtgctcga tcaggctggc accgacctcg ccggtgccat tgagcacgtt
    22921 gaacacgccc gccggcacac cggcctcgat cgccagctcg gccaggcgca gcagggtcag
    22981 cggggtgaac tcgctgggct tgagcaccaa ggtgcagcca gtggccaggg ccgccgcgat
    23041 tttccagatg ccgatcatca ctgagaagtt ccacggcacg atgccggcca ctacgcccac
    23101 cggctcgcgg cgggtgaagg cggtgtactg ctcaccggcc atggacggga acgacggggt
    23161 catggtctcg ccattgatct tggtcgtcca gccggcgtag taacgcagga agatcgcgct
    23221 ctggccgact tcgaacatgc gcgagaggtg gatggacttg cccgagcaga gggtttccag
    23281 ctgcgccagt tcctcgccgt tggcttccat caggtcggcc aggcggttga gaatcacacc
    23341 gcgctggtag ggcgaggttt gcgcccattc gccttcgaag gtgcgccgtg cggcggcgac
    23401 agccgcctca acctcggcgc tggtggcaca ggtgatctgg gcaatcactt caccgttggc
    23461 cgggttgacg atcggcacgc gtttaccgga ggtgccggcg cggtattcac cgtcgatgaa
    23521 atgggcatgg tcgcgctgaa ggaactcgcg cacctggggc agtaactgga tttcactcat
    23581 gaaggactcc gggggtgggg cgggggcagc ccgaggttct gcgcctctac gggccggctg
    23641 gggtgtccgg gcgccgatgg gcccgggccg ggaatatatg ggcgctattc atagcacccg
    23701 gctggggtgg ataaattgat catggtggac aggttgttgt agatgcctgc caccttggca
    23761 gcgtcgcccg gctcagtggc gggcagctct aacccgcgcc caggcctcat ccagtacctg
    23821 cttttgctgg ggggatagac tttccagcag gtagctgcgg cgtcgctgct cggccgtcaa
    23881 cacttgcacc ggatgctcgc tggcgaaggc ctgcataagt ggcgatgcgc tgggcagcgg
    23941 cgggtaaaaa tgggtcgcac gggcgttacg aatggctacg gttgggtcga taagaaagtc
    24001 gatcaggcgg taggccaact gcggatgggc ggcatgggtc ggtattgcca gggtgtcgat
    24061 atacatcgcg gcgccttcgt ccgggatttg gtagcgcagc tgcggattgc cttccatcgc
    24121 gcgtgccgca tggccactcc aggccatggc cagacacagt cggccggcaa tcagttcgtc
    24181 gacgaaattc cagttgtcca gcgtgcgcaa cagcggtgcg atggtctcca gcccgcgtag
    24241 ctggcgggcg atctgtcgat tgctgctggc cgacagacgt cgtccgcggt agttgagtag
    24301 cagcgagccg gtttcttcgg cagcgtccag catgccgagg ccgcaggggc tgagcttggc
    24361 ggcctgctcc gggtcgaaca gcagacgcca gctgttgggc agcgtaccgg cgtaatgtgc
    24421 ccgggcctgt tgtgggtcga ccaccaggcc gatggagccc cacagatagg gcacggcatg
    24481 acggttggcc gccgggatgc cagccagtgt tgacagcagc cagggatcga ggctggtgta
    24541 atgtcgcagg cggcgcgtat ccagtggcgt caggtgaccg ccggcgatca gctggtcgag
    24601 catgaagtgg ctgggcatca ccacgtcgta atcggtgccg gcggccagcg cctcgagcag
    24661 ttcgtgcgtg gtggtgaaac tctggtaatc gacagtaata ccgctgtgcg cttggaagtc
    24721 ctccagcacc tgtgggtcga tgtagtcctt ccagccgtaa acgcgcacga cttcttgggc
    24781 gtgggcgtgg gcggtgctgg cgaacagcaa cagcagggcg aatagcagct tcatcggatg
    24841 gctccggtca ggtcggacga cgaaggtcgc cggcagtggt cggcagaccc gtgtagggcc
    24901 attcaatcgc cctctgccgc gcgtcgcttg ttgctggcag ccaagttctt gtacatggct
    24961 gccagatgat gtcagccgtt cagaaagcgg cgtgccaggc ccagttgcga ctcggccaac
    25021 tggtgcaggg cctggctgat ctcgttggta cggccagcct gctgactggt gcgttcagtg
    25081 aaaccggcga tatcggtgat ctgctggttg atctgttcgg cgacctgtcc ctgttgctcg
    25141 attgcggcag ccatttgcag gttcatgcca ctgatctggg taatctcgtc gcagatgcgc
    25201 gtgagcgcct ggcgtgcctg ctgcacgtcg gtgctggcgc gcagggcggc cacctcgccg
    25261 cgttgggcgg tggccagcgc cgtgccgctg tcgtgacgca ggctctcgag cgagcgctgg
    25321 atctcgtggg tggattcgcg ggtgcgcgag gcgaggctgc gcacttcgtc agcgaccacc
    25381 gcaaagccac gtccggattc gccagcacga gcggcctcga ttgccgcatt gagggcgagt
    25441 aggttggtct gttcggcgat gctctggatc acctcggcaa cgctgccgat tgattctatg
    25501 gcggcggcca ggctgccaac cgcctggcct atctcaccaa ccgagttgtg catggcctgt
    25561 gccgagcgct ggctgtcgtc ggacatgcgt tcgccgtcgc gggccagttg ttcggcgttc
    25621 tgcgcggcct gcgcggtgtg ctgtaagttg cgcgagagtt cctggatggt agcgctcatc
    25681 tgattgatcg cgccggcgat ctgctcggtc tcgcggacct ggtgttgcag ttgctcgacc
    25741 gagtgctcca ccacgtcggc cgaggtctgg gcctgctggc gtagtaccac gctgttgatg
    25801 cagatgcgcg acatcactgt ggtcatgcgt gcacgctggc tgtccagagc catggccagc
    25861 aagcccgccg ggccgggttg ttcggcgtag agcggggtga gcagcgggtc gctgtaaacc
    25921 tgggcatgct gggcgagaag ggtgcggctc aggcggcgct ggtgcagcac gggctgtagg
    25981 ccggcgagcc ccagggccag caacaatggc acgcccactc ctgcggacag gtatccctgc
    26041 agtgtgacca gtgccagacc caccgcgagc accagttgca ggccgttgcc cgccagcaac
    26101 gcagcgcaat gctggactgg cttccacggc gcctgtcctt gcgccaggcg cgcatagagg
    26161 ctctcggcac ggcgtgccag ttccggctcg atgggctggt acacggtgcc cagggctacc
    26221 agcttgccgt cgtcgaacag cggcaccacg tacaggttgt gccagtcccg attgccctgc
    26281 ttgtggcgaa tcatcactgg cgctgtccac ggcacgccgg cgcgtaacgc gcgccacatg
    26341 ctggcgatca ccgacgcagg catgtgcggg tggttgacct gttcgtgggc tttgccgatc
    26401 agctcctgtt cggtgaaacc gctcatgtgt aggtaggcgg cattgcaggt aagcagaacg
    26461 ccctgtggat cgagccggga aatcggcggc tcgtcgaaac ctggtggggt gttgttggca
    26521 gtttgcatga cacgactcct cagctgtgac ctgttgcggc ctgcttggca agccgtgcga
    26581 tgacgtccgg cagggcttag gtggcgagca tgtcctgatg tttgctggcc aggcccaggt
    26641 aggcttcgat cacccgcggg tcgtccgcca attgtgcgga cggaccctgc atggcgatct
    26701 gcccggtttc cagcacgtag gcgtagtccg ccacgcgcag ggcggcgcgg gcgttctgct
    26761 ccaccagcag gatcgacacg ccctgctggc gcagggcggt gatgatgcgg aagatctcgc
    26821 gggtgatcag cggtgctagg cccaggctcg gttcgtcgag catcagcagc ttgggcttgg
    26881 ccatcagggc gcggccgacg gcgagcatct gccgctcgcc accggagagg gtggcggcca
    26941 gttgcccacg gcgttcccac agccgcggga acaactcgta gacctccttg agtgtctggc
    27001 cgtgatcacg ctggccgctg cggtggcgct ggaaggcgcc gagcagcagg ttgtcggcca
    27061 ccgtcatgct gctgaacagc tcgcgctttt ccggcaccag gccgaggccg cggccgacca
    27121 tcacctcgac ttccggcacg gcttccaggc tgccatcgaa ggcgacccga ccacgggagc
    27181 cgagcacgcc catgatggcc gagagcaggg tggtcttacc ggcgccgttg gggccgatca
    27241 ccgtgacgat ctggccctgg ccgacgcgca ggctggcatt gctcagcgct tccaccttgc
    27301 tgtaggcgac gcacaggtcg ctgacctcca gcaccgggtt ggccacctgg ttctgcgcgt
    27361 gctgctgcgt gggcatgttc atcactctgc tcctccgaga taggcttcca gcaccgctgg
    27421 gtctttctgt acatcggccg gcaggccgaa ggcgatgcgc tggccgaact ccatcaccac
    27481 cacgcgatcg accaggccca tgacgaagtc catatcgtgc tcgaccagca ggatcgctag
    27541 gccttcgctg cgcagacggc tgagcaactg gccgagggtt tgcttctcct tgtggcgcag
    27601 gccggcggcc ggctcgtcga gcagcagcag gcaggggttg gcgcacagcg cgcgggcgat
    27661 ttcgaggagg cgttgctggc ccagggccag gctgccggct tccaggtaca ggcagtcgcc
    27721 caggccgacc cgttccagtt gacggcgcgc ttcggcgagc aggcgtgcct cctcggcgcg
    27781 atccagacgc agggcggcgg cgagcacgcc cttgctgccg cgcaggtggg cgccgagggc
    27841 gacgttctcc aacacgctca tctccggcag cagcttgacg tgctggaagg tgcggctcat
    27901 gctcatgtgc gcgatgcgcc gcgagggtat gccgttgatg cgctggccac ggaacagcac
    27961 ttcaccggag gtgggcgtgt cgacgcctga gagctggttg aacagggtgc tcttgccggc
    28021 gccgttgggg ccgatcaggg cgagaatttc cccggcgtgt acctccaggt tcatctcgtt
    28081 gttggctacc aggccgccga agcaacgggt cacgtcgcgg gcttccagca gcacctcgcc
    28141 gacgaccggt gcttcgcgct gcggcagtgc gctggcgtcg tccaccggaa gtgccgcgcg
    28201 gcgggctttc cagctacccg gcaacaggcg cgtaaacagc ggccagagac cgccaggcgc
    28261 gcgctgcatc agcaagacga tggcaatgcc gaagacgatc acctcgtagt tgccggtatt
    28321 gcccagcagt tgtggcagca gatcctgcag ccactgcttg agcagggtca acgcgccagc
    28381 gccgagcagg gcgccccaga cgctggtcac gccgccaatc agggccatga acaggtactc
    28441 gatacccatg tgcaggccaa acggcgtggg gttcacgaaa cgctgggtgt gggcgtagag
    28501 ccagccggag agcgcggcga acaaggccga gatcaggaat accagcattt ttgtgcggaa
    28561 ggtgttcacg cccatcgact cggccatcag ctggccgccc ttgagcgcgc ggatggcacg
    28621 gccttcgcgc gagtcgagga gattctgggt gaggagcatg gcgcccagca ggatggccca
    28681 gatcaggtaa tagatcttct cgcccttatc caacacccag ccgaacaggg aaatactcgg
    28741 caggccgctg accccggtgt gcccgccgag cgactccagg gtgccgaacc ggtagtacag
    28801 cgacaggccc caggcgatgg tacccagcgg caggtagtgg ccggacagct tcagcgtcag
    28861 cgcgccgagc accagggcca caacggcggt cagggtcagc ccgaccagca ggctcagcca
    28921 gggcgaggcg ctggtccagg ccagccagct tggcaggtcc tgcacggtgg tcaggtaggc
    28981 gctggtgtag gcgccgaggc cgacgaaggc agcctggcca aagctggtca tgccgccgac
    29041 gccagtgagc agcaccaggc cgagcaccac cagggtgtac aggccgatgt agttgagcag
    29101 ggtgaggtag tagggcggca gcaccgctgg tgccacggcc agtacggcaa tcagggcgag
    29161 gatcaggtag cgcggtttca ttcatcgtcc tccacatggc ggctggtgag ggagcgccag
    29221 agcaggaagg ggatgatcag ggtgaagacg atgatctcct tgtaggtgct ggcccagaac
    29281 atcgagaacg cctcgatcag acccacggca agggcaccga gggcggccgc cgggtagctg
    29341 accaggccgc cgatgatcgc gccgacgaaa cccttgaggc tgatgacgaa accggagtcg
    29401 aaatacaggg tggtaatggg cgcgataagg atgcccgaca gtgcgccgat aaaggcggcc
    29461 atggcgaacg tggccttacc cgccaggttc ggtgaaatgc ccatcaaccg tgcgcccatg
    29521 cggttcactg cagtggcgcg cagggccttg ccatacaggc tgcgttcgaa gaacaggaac
    29581 aggctgatga tcagcgtcag ggacacggcg atgacccaca gggtctggct gttgaatgtc
    29641 actgggccga gcgtcaggcc ggcttcggag aagggttcgg tgcgcgcacc ctcggggccg
    29701 aacagcagca gggcgatgcc gaccatggcc acatgcacgg cgatggagac gatcaacaga
    29761 accagggcgc tggctgcggc caggggctgg aacaccaggc ggtaaagctg cgggccgagc
    29821 ggcaccacca gcgccagggt aagcagggcc tgcacggcca tgggcagctg cgccagtggc
    29881 aggttgtgga ccaggccgag catggtcagg gcgtaggcga gcttgagcgc gatgcgtcgc
    29941 ggcaagcgga aacgccggct ggcgcgggcg gcgtcataca gatccatggc gcagtcggcc
    30001 agggtcatgg ccagcaacag ccagaccagc gcggtggact gaccggcctg cagggcggcc
    30061 atggtcaacg cgccgtaggt gacgaactca cccatgggga tcagcaggat gcgtgtcacg
    30121 gtgaagacca gcaggatcga caacgccagc agcgcgtaga tcgcaccgtt ggtgatgccg
    30181 tcctggccga gcagcaaggc aatctgcacg ttcatatcag ggactcttgt gcggtatggg
    30241 tatgcgggct ggtgcggcct ggacgtaggg tggatagcgc tttttcatcc acctgccggc
    30301 gccgcaccag gctccatccg gtggacagaa aagtgctgtc caccctacag ggccggtgtc
    30361 cgcgatcagt ggctgccgag cagcgtccag ttgccgttct tcacctggat cagctcgcga
    30421 ccgcgctcgt cgaatccgct gtgatcctca ggactcatgt tgtagacgcc ctgagtggcc
    30481 gcgaactcgt gggtcgcctc cagtgcgtcg cgcagggcac tgcgaaactc gggcgtgccg
    30541 ggctcggcct tggcggcggc ggccggaatc gcctgttgca gcaacagccc ggcgtcatag
    30601 gtattggcgc cgaaggtggc cggctggctg ccgttgagct tctcgtaggc ggccacgtac
    30661 tcagcggcga tggctttgga aggatggctg tcgggcacct gttcgagcac cagcatcagg
    30721 ctggcagcga gaatggtgcc ttctaccttc ttgccgccaa gcctgaggaa atccggcagg
    30781 gcggcgccgt gggtctggta catctgccca cggtagcctt gatcgaacag ggttgtttgc
    30841 ggcaggaccg ctgcgctgcc cggcgcggcc accagcacgg catcgggccg cgaggcgagg
    30901 atcttcaggc tctgcccggt gacagaggtg tcctggcgct ggaagcgttc gctggcaagc
    30961 atcttgatgc cgtgttcggc cgccagtgcg gccatcacct tcgaccagtt ctcgccgtag
    31021 gggtcggcgg tgccgataaa gccgagcgtc ttcacgccac gggcgttcat gtcctcgacc
    31081 aacgccttag cgatcaggtc gtcgttctgc gtggtcttga acacccactt cttctgctcg
    31141 gtcatcggca gcactacggc ggcggtgccg accggtgcca gcaacggcac gccggcctcg
    31201 gcgacgaact ggatcacgcc catggcattg ggcgagccgc tcgggccgat gatgacatcg
    31261 acgttttcct cgctgatcag cttcttcagc gccttcactg tggcggtggg gtcactgccg
    31321 tcatccaggg cgatgtattt cacctgctgg tcgccggcct ggtcgggcag cagcgctatg
    31381 gagttcttct gcggcagccc gaccagagcg atggggccgg ttgatgaggt gatgacaccg
    31441 acctttacct cggcctggac gaaggaggcg cagacggcgc tgagaagcag gctggacacg
    31501 gctcgtttga agtgcatgac gtactccgag gaggcaaggc tatttggaaa gacagagacc
    31561 taccggatgg gagggaatgt cgtcttgttg ttctggcgcc tttgggcgca gccggtgatt
    31621 tatccgttcc gacgcagcct gttttgcacc tcccgtgcca gaacgaggtg cgcctgaggg
    31681 cgcgcatcgc tgttcagcgt tgtagttgaa cgtcctgact ggcgccggca gggccgtctg
    31741 gaggcgccct ttcgccgaga gcggtgaggc gtcccaaggc cgatgtcatc aggcgtggag
    31801 gcgcattttt ataggctttt aaacgtcatt tttacaatgt tttatgttct aaaaatgccg
    31861 ttttagaagc taaaaatcgg ccttatagca aggtattttc tgtgcgatta tcggtcattc
    31921 accgctgtgc aataaatcgg caagatgacc tggagaaaat tgcatgatgc ctgaatttat
    31981 agataaatta ttgttctata agaataatta aggattatcc gtgctcgtga tgagtatttt
    32041 tggtgttacc aaattgcacg gcaatggtgc tcggcgtggc gaaagtgcac cgaatctaca
    32101 cactattttt agtggataat tcaattgaca attcacgtaa atattccgat tctttattta
    32161 cgcaaaccgc tccactgagg tggctcggtt ttcaacaaga acggcagtgg gtgaagcaga
    32221 tggcgatagt ggtcgagaag ttgcaactcg gtgggcaggg ccttcgcgta atggtgaagg
    32281 acaccattga tgtggccggt catcccaccc gtgcttccag tcgcgctctg caacaggcgc
    32341 cgcgtgctga gcgtcatgcc gatgtagtcc aggcgttgct cgacgccggc tgccaactgc
    32401 tgggcaagac cagcctgcac gagctggcct tcggtaccac cgggctcaat gcctggacgg
    32461 gtacgcctga gaacccacgc tatcccgggc gtattcctgg tgggtcctcc agcggttcgg
    32521 cggctgccgt cgctggcggt ctgtgtgatt tctccctggg caccgacacg ggcggttctg
    32581 tacgtatacc tgctgcctgc tgtggcgtgt tcggcctcaa gcccaccttt ggccgtatca
    32641 gccgcgctgg ggtgatgccg gcgcagactt cgctggactg tgtcggcccg ttcgccgccg
    32701 atatcgagac gctgatcgcg gcaatgagtg ccatcgatcc ttccttcaag accctgcctg
    32761 ctgtgaatgg catctgcatt ggtgtggtgc cggtcgaggc ccacccggaa gtgcaggccc
    32821 ttctgggcgc cgtgctcgaa gcctctgctt tcgatctgcg cagcgtgccg ttgcccggta
    32881 tgcaggccgc ctacgacgcc ggcctggcgg tgatcaatcg ggagacctgg aatgcctgtg
    32941 ggcacctgct ggaaaccggc ctggtcggca gcgatgttgc cgctcggctg ctggctgccg
    33001 gtgacaccag cgaagccgct ctggccgatg ccgaacgctg ccgtgcggcc ttcaccgccg
    33061 aggtggatgg cgcgctgagg cagtgcccga ttcttgccct gccgaccatg ccggagtacc
    33121 cggcacttgt cgcagaggct gccgataccc gtgcggccat cggtatgacc gctttcgtac
    33181 gccctttcaa cctgactggc cacccggcgc tgagcatccc tttcgtgggg gcctcgctgc
    33241 taccggtagg actacaactg gtcgccgcca aaggcgcgga cgaactgctt ctggcagtcg
    33301 cccgcgagtt gctgcagcgc ctcgaacaat aaccgtgccc accctggaga agcccatgtc
    33361 ttccctaagc cagctggatc accagcgcct tgccccgatc accgcgaatg aagcctttca
    33421 agcgctgctg accgatatcc gcgagcgcgc ccggcgtgaa gagtttgacc aacagcggtt
    33481 catctcccac gacgtgatcg agcgcttcaa agcgctcggc gtctaccgcg ctctggtgcc
    33541 cacccgcttc ggcggcgagc agcgcagccc catggaattc tgcgagatgg tggaggaggt
    33601 ctccgctgcc gacggttctg ccggttgggt cgccagcttt ggcatgaatc cggtgtacct
    33661 cgccgccctg ccgctggcga ccctggacaa ggtgtacgcc aacggccctg acgtggtgtt
    33721 cgctgccggc atcttcccgc ctcagcccgc cgaaaccatt gagggcggtt tcaagattcg
    33781 cggccgctgg aagttttcca gcggctgcaa gggcgctgac ctggttggtg tgggcattag
    33841 ccccaaggtg ggtgaaacgc attcgctgcc gcgggtggcg gtcgtgcccc aggctcaggt
    33901 ggttatcgat gaaacctggg acatggtggg tttgatcggt acggggagcc atgacctggt
    33961 gatcgatggc atggaggtgt ctgaggagtg gaccttcgtt cgcggtggcc aagccaatct
    34021 agacgagccc ttgttccgct acccgtcgct gtctttcgcg actcaagtgt tgtccgtggt
    34081 cggtttgggt gtggcccgtg ccgcactgga ttacctcacc gacatggcca gcggccgctt
    34141 ctcggtcact ggcgcccccg tgctggccga ccggccgttg gcgcaaatgc agatggccaa
    34201 ggctgaggca gagttacgcg ctgcccgctc ctggttctat gaagccatgg agaacgcctg
    34261 ggccagcgtg ctggcaggcg acgcggtgag caccgagcag accaatctgc tgcgtctgtc
    34321 ctcaacccat gcggtacggg tatcggcaga ggtcgcgcgc tctgcgcaga tgctcgcggg
    34381 catgaccggg gtttgccgta acagcccgct tgcgcgcttc gttaacgacg cccaggtggt
    34441 cacccagcac gcctttatgg gcgacatgac ctaccagaac gccggcgcga tcttcttcgg
    34501 cggcaagccg atgcccggct acctgtaatc gacaactaac catcggtgac tgtgatgact
    34561 gagaaaaaat ccctgcgcgt gctgttctgc atgggcatca accagaactt cttcgacgct
    34621 gcgcgtgaag agcagctgga agtctgggcg gccttcagcg ccatgtggaa cggtatccac
    34681 gacctgcccg gagtgacggt ctacggcaac atggatgacg accagagcat ggtcggtccc
    34741 agcacgggct acccctggac cacctacctg ctggccgacg tgccggacat cgaaaccgtc
    34801 cacgccgcct gcaacctgtt ccgcaccacc gcggtgggcg ctggccccta caagctgtgg
    34861 cgctactgca aagtcgaggc ccgtaccggc cgcgaactga ttatccagcg ggcctgagcc
    34921 atgagccgaa ccctcgaagc ccgcctgcac cagctggaaa gcgagaacgc cgtacgcgcc
    34981 tgcatgaacc gctacatggt gctgtgcgac gcacttgacg cgcgcactcc gctggacgag
    35041 ctggccgggc tgttcagcgc cgatgccatc tgggaaggca agggtgccaa gtacgccaag
    35101 agcttcggcg gctaccgggg gcgcgaggcg atccgcgcga tgtttgccgg ctacatgagt
    35161 gagcccgcgc acttcgccct caacgtgcat tttctctgct cggagcttat ctgcgtggag
    35221 ggtgacgagg ccagtggcag ttgggtgatg ttgcagacct ccaccttcgc cacgggtgcc
    35281 tcacacctca acgccgcacg cctgacggtg cgtttccgtg aggaggaggg cgcctggcgc
    35341 atggcccatt ttcagaccga gaacctgttc ggccgcccgg tcgatgcatg gaacagcgat
    35401 gcccacctac ccgtaccggg gcagcgtgac gaatgatttt ttgagtcggt gacctgcacc
    35461 gacgaggagt gaccgtgacg aacctgatcg aaaccgtgaa cctggccagc tcgccggccg
    35521 acctggtgct gcacgaccgc gtgcacacct ccctgtacac cgatgcgcgc ctcttcgacg
    35581 aagagttgca gaaggtgttc tacagcacct gggtgtgggt cgcgcatgcc agcgagattc
    35641 ccgacaacgg cagctacaag agcacctaca tcggcaagca gccggtgatt gtggtgcgcg
    35701 accgcaagaa ggacgtgcac gtactgctca accgctgccg ccaccgcggc gccaccgtct
    35761 gtgagcacaa aaagggcaag accaacagct ttgtctgccc ttatcacggt tggagctacg
    35821 ccctcgacgg cagcctgcgc ggcgtgccgc acccggagag ctacgacgac tgcctggaca
    35881 agggcgagct gccgctggtc agcctgcgcg tcgagcaata caacggcatg atcttcgcca
    35941 ccttcaagga tgacatcgaa ccgctggtgg acttcctcgg cccggcgaag aaatggatcg
    36001 acctgtttat gaagcagggc gccggttacg gcgtgaaggt ctccggtgag catcgcttcc
    36061 gcttccccgg caactggaag atccagctgg agaacaccac cgacgcctac cacttcccgc
    36121 tggtgcacaa gtcgttcctg tcctcggtgg acgagcagac cctggagctg ttcgatttcg
    36181 tcaaaggacc cggctacgtc gaggacctcg gcaacggcca cagcgtcatg gtgatgatcc
    36241 ccgacctggt tgacctggag gctgacctcg acaagccgat ccccgagcgc ttcgaggcgc
    36301 tggcgcagga gctgcgtgac gaaggcatcg atgagcaaca ggtgcggcgc atcgtccgcg
    36361 ccgtcggcgg ttcgggcttc aacctcaacc tgttccccaa cgtcgcctgc tccatggcct
    36421 tcttccgcgt gctgcaaccg gtctcggtgg acgagaccga gatccaccat gcggtgatca
    36481 ccatggacgg cggcccggcc gtggccaacc gctaccgcct gcgtctgcac gagcatttcc
    36541 agggcccgat gggctttggc accccggacg attccgaggc ctgggagcgc gtgcagaagg
    36601 gcacccaggc cggcagcgac ctgtggatca tgctcaaccg cggcctgccc ggcgagaagc
    36661 cgagtgagga tggcctggtc agcgacgtga gcgccgagac cggcatgcgc gcggcttacc
    36721 agcagtggaa aaagatgatg tcggcctgag ccatttcgcc cctctctgcg cgcgatgaga
    36781 ggggctagct gctcagatcc gtaacgcacc tatttgaaga gaacgaccat gaatcttgaa
    36841 ctgctcaacc acgtcagtgc ctttatctgg caggaagcgg acatgctcga ccacggtgaa
    36901 ttcgacgcct ggctgaagct gtgggccgac cagggcacct acatcatccc catcgaccct
    36961 aacgagaccg acttcgagaa caccctcaac tacgcctatg acgaccatca tatgcgcgag
    37021 ctgcgggtgc agcgtttgat cggtggcgag tccatttcca ccagcccgcg ggcccgcacc
    37081 gtgcgcagcc tgtcgcgctt tcgcgtactc ggcgagaccg acggcgtggt caccgtgcgc
    37141 tgcgcgcaga acctgcgcga gttccgcaag gacgtgctca agcactacac cgccgacatc
    37201 acgttccagc tcaagcgcga cggcgccagc ttcaagatcc agcgcaagct gatccagttg
    37261 atcaactcca ccgataccct cacgggcatc ggctacatcc tctgaggagc gcagcatgac
    37321 ccaggtagcc ctcgtcaccg gcgccgctca gggcctcggc ctggccatcg ccaaccgttt
    37381 gtttgcagcc ggttacgcgg tggtgatcac cgacctgtcg ctgcagcgcg cccaggccgc
    37441 cgtcgcccag ctcgacccca gtggccagcg cagcctggca ctgcaactgg acgtggccag
    37501 caaggctgac ttcgaggcgg cgctggccgc cgtgctggcc cgctggggcg cgctgcatgt
    37561 ggcggtcaat agcgccgcga tgacgcgcac cacaccggtc atgcagatca gcccggaaga
    37621 attcgatcag gtcatgagcc tcaacacccg cagcatcttc cttggttgtc aggtgctggg
    37681 tgggtacatg gcgggcgctg gctacgggcg gattatcaat atggcgtcgc tggccgggca
    37741 gaatggcggc actgctaccg gggcgcatta cgccgcctcc aagggcgcca ttgtcaccct
    37801 gaccaagata tttgccaaag agctggcggc tgcgggtgtg acggtcaatg ccatcgcccc
    37861 tggccccatc gagtcgcctg cggtgcatgc cgccgtaccg cctgagcgca tggccggact
    37921 actggccaac atcccggtga aacgccttgg cgatgccgat ttcctcggtg acctggtggt
    37981 gcagctggcg cgcccggaag cctacttcac caccggggcg acctgggacg tgaacggcgg
    38041 cctgtttatg cgttgaaccc cccgcccggc atggcctcgc ggccgagtcg ggcagaccaa
    38101 agaatgccgc gcgtgcgagc tgcggcctgt aacgaacgag aacaatcagc accgcgaaag
    38161 gtgcaggaag gtcggtatga ctgaacacgt gttgaacctg gtggtacgca agcggcagga
    38221 gcaaggcgag ggcgtggtaa tccttgacct cgccgatccg atgggcagca atctgccggc
    38281 cttcgaagcc ggtgcccatg tcgatatcca cctcaagccc ggcctggtgc gccagtattc
    38341 cttgtgcggt gatccggcca acgctgccgt ctaccgtctg ggtgtgctgc gcgacccagc
    38401 ctcgcgcggt ggctcggtgg cggttcatga cctgctcacc gagggcgcca gcgtcagcgt
    38461 cggtaccccg cgcaacctgt ttcccctggc cgcaggtgcc agccgctcga ttcttatcgg
    38521 cggtggtatc ggcatcaccc cgatgattgc catggcgcac cagctgaccg cccaggacag
    38581 cccgttcgag ctgcactatt gcggccgttc gcgtcaccgc acagcgtttc tcgatgaact
    38641 ggccagcgct gacttcgccg cctgcgtaca cacccacttc gatgaggaag atgctgcgca
    38701 gaagctcgac ctaaccgccg tactcggtca gccagatgct ggcctgcact tatatgtctg
    38761 tggtccggcc ggctttatgg actgggtcat cgagggtgca cgcaaggctg gctacgccga
    38821 agaacagatc caccgtgagt actttcaggt ggaggtggac agcgctggcg agagcttcga
    38881 agtggtggcg cagcgcagtg gcaagagcgt tcaggtggct gaaggtcaga ccatcatcga
    38941 tgccctggcg ggcattggca ttacggtgga gatctcctgc gagcagggcg tctgtggcac
    39001 ctgcctgtgc gatgtattgg aaggcgagcc ggatcaccgc gacgtctacc tcaccgacga
    39061 cgaaaagacc gccaatgacc agatattggt ctgctgttcg cgcgccaagt cgaaaaagct
    39121 ggtgctggat atctgaaaac tgagggcaag atcatgattg ataacacagg tttccgtaat
    39181 gccatggcgc tgctgggtgg cgccgtttcg attattacca ccgatggcgc tgctggacgc
    39241 tggggattta ccgcctcggc ggtatgcagc gtcaccgacc aacccccgac gctgctggta
    39301 tgcatgaacc gttcgtcctt ctcgaatatt catttcaaac aaaatggcgt gctcagtgtg
    39361 aacgtgttga gcgccgagct gaaagacatc tccggggtgt ttgccagccg tgatctggac
    39421 tccgatcagc gttttgccca ggcaagctgg agcaccctgg agagcggcgc gccactgctg
    39481 gatgacgccc tggtcaattt cgactgccgc atcgcccagg cgcacgaggt gggttcgcac
    39541 accatcttct attgcgaggt gctgggtatt cgtaacggca agagtcagga cgggctggtg
    39601 tatttcaacc gcgcctatca ccgcttgggc gagcagtcgc gctcggcctg ctgatacgcg
    39661 gcgggttatt gccccgctac tgtttgccag tgtcacaggt cgttccgagc ttatttcagc
    39721 atgacggatg aatcgataat tcatccccaa acatgcaatg cccgttaata agaaacgact
    39781 gcccaatcgt tatgggtagc gccttgccac gacccttttg cagtaagtgg ttataaaaat
    39841 aaaactgcgg attaactgac taagttgcct tttctgccaa ttaatctgct cccactcatt
    39901 gtgtcttgca cgcgtaataa aggatcaaaa caatgtctca gaacaactgg cggacgggca
    39961 gcattggcgc tgttggcctg atggctgtct tcgcgtcgat ttccgctcag gcggacttca
    40021 tcaatgatcg acacgtcagc cttggcttgc gcaactttta tatcgaccgc gacttcaagc
    40081 agcacgatgc gcccaagtcg cgtatcggca gctggaccca gggtttcgat tttcggtcca
    40141 tttccgggta taccgaaggc actctgcaat tcggtctcga cctctctgcg caatatgcct
    40201 atcgcctgga tggcgggggt ggtcgtggcc cggacagcat cattccctac gatgaccgca
    40261 agggtgagca ggtgcgtgac tatggccgcg ccgcactgac cgccaaggtg cgttactcca
    40321 agaccgagct gaagatcggt gagcatcggc cgatgctccc ggtagccttc tacgatgact
    40381 cgcggcaact gatcaccacc taccacggct ttatgctgga gtcgcgcgag gtggacaggc
    40441 tgaccctgac cgccggccgc ttcaccgaga tcagcagccg cgagtcatcc aaccgcgagc
    40501 agatgtacct gttcaatggg ccggatatcc agcgccgcag cgatggcctg aacttcgccg
    40561 gggccaccta cgcgttcacc ccggcactgt cggcgagcta cttctacggt cagctggaag
    40621 acatctatca acagcagtac ctcggcggca cctacaacgc tgacctgggc aacggcttcg
    40681 gtctgaagac cgacctgcgc tacttcgaca accgtgagga cggcaaggcg ctctacggtg
    40741 acatcgacaa tcgctcttac ggcgtgatga ccaccctgcg caagggcgcc cacgccatcg
    40801 gtttcggcta ccagcgcatg cttggcgaca gcaccttccc caccctcaat ggctacgtgc
    40861 cgcagccgta tctggtgaac tggtccaccg tcgccttcgt caagcccaac gaaagctcct
    40921 ggcaactgcg ctacgacctg gacttcgcca gctatggtgt gcccggtctg aagctgatga
    40981 cccgctacct gcgcggctcc ggcatcgatc ggggcaacaa cgacctggcg cagaacatgg
    41041 aaagcgagcg caacatcgtc ctcggctacg tggtgcagga cggcccgctc aagggcgtgg
    41101 ccttcgagtg gcgcaacatt gacgtgaaga cccgctacgg caatggcgct gcttccgggg
    41161 ccgactacca ggagaatcgt ctgataacct cctacacctt cacgttctga ccctcggcgg
    41221 ggccgggtgc agatccgacc ccgccagcat tcaatatctg cgagaacacg atgcgactct
    41281 acgaactgat taccttcacc ttgcgcgtgc gcaccccggc cgaagcgctg cagcgcctgc
    41341 gcgaaaccct cggcgaagca cgcgatggct gcgtgctgat gggctgctgg gcgtcggaaa
    41401 tcggcccgct caaccagatc gccgtgctgc gtggcttccg tgacgaaacg gcccggcagc
    41461 acgagcgcga gcgcttcctc caggcgccgg atgccttcgg catcgaaacc tttatgctcg
    41521 atatgaaagt ggagaactac acgctgtttc cctttctcga gcccctggcg cctggcaagc
    41581 acgggccgtt ctacgagttg cgcgagtaca acctgattcc atccggcctg gcccccacga
    41641 tggcaggctg ggaaaaggcc gtcggtccgc gcaccgggga gggctattca gctgtctatg
    41701 cagctttctt cgccaccgac gggcgcacgc cacgctacct gcatatctgg ccctacgcga
    41761 ctcaggagca acgcctggac gtgcgtaccc gggcggtggc ggacggcgtc tggccaccgg
    41821 agaactccgg gccgcaactg cgcgaaatgc attccaccct ttacctgccg gcggtgtttt
    41881 cgccgttgca gtagggacgg gtgacctttt tcatgcgtgt ctttagcccg tccctccccg
    41941 tgggcttttt tcttcctgtg ccgtcagccc tggcgtgcgc gtgacaactc gtccaccagc
    42001 gccaggcagt gctcgagcgc tggcgaggcg tcgccgacgc gccggctgat gatgatcggc
    42061 gaggtggcct gtgcttcctg cagcggcgcg tagtcgatat cggcgcggtg cagcacctgc
    42121 accgaggcgg gcaccagggt cacgccaata ccggcagcga ccagaccaat ggccgtttgc
    42181 aattcattgg tccattgcgc cacgcggatt tgcaggccgt gcgcagcgaa caatgccatg
    42241 acatggtcgg cgtagctcgg ccgtggattg cccgggtaca gcacaaatgg ctcgcgggcc
    42301 agttcggtca ggctcaccgg gccgggcagc aacggatggc ccgcgggcag agccgccacc
    42361 agcggttcga gggtcagcac ctgctggtgg atcgctgcat catcgatatg gatacggcca
    42421 aagccgatgt cgatgcgtcc ggccttgagc gcttccacct gttgcaccgt ggtcgtttcc
    42481 gaaaggccga gctccaggtc gacgtcactg cgcaggcggc ggatcagctc tggcagcacg
    42541 ccgtaaaggg tggagggcgc aaagccgatg ccgagccagg ctttctcgcc agtaccgata
    42601 cgccgggtgc tctcgcagac cttgcccagt tgttcgagca gagtgctgga gtgctcatgg
    42661 aagaagcgcc ccgcctcggt caggcgcagc ggccggccgc gttcgatcag agagacgccg
    42721 agttcgtcct cgagctgctg gatctgccgg ctcagagggg gctgggcgat gtgcagacgc
    42781 tcggcggcgc gggtgaaatt gagggtttcg gcgagtgcct ggaaatagcg cagatggcga
    42841 agttccacgg gtggttgtcc tatgcagttg ataataccta aagggtattg ttgcagacaa
    42901 aattaatatt ggaacctcta caagcgcagt atcaatatca gggtctggat aaaaacaacc
    42961 tgatgggtat tgaaatgagc cacgccgcga tcgagtcgct cgaagcgatc atcgtcgacc
    43021 tgccgaccat ccgcccgcac aagctggcga tgcacaccat gcagaaccag accctggtga
    43081 tcctgcgcct gcgctgcgcc gatggtatcg agggtatcgg tgaagccacc accattggtg
    43141 gcctgtccta tggcaatgag agcccggaca gcatcaaggt caacctggat cgtcacttcg
    43201 cgccgctgct gatcggccag gacgccagca acatcaacgc ctgcatgcag cgcatcgacc
    43261 gcgtggttaa gggcaatacg tttgcccgct cggcggtgga aactgcactg cttgatgccc
    43321 agggcaagcg tctcggcctg ccggtcagtg aaatcctcgg cggccgcgtg cgcgacagcc
    43381 tggaagtagc ctggaccctg gccagcggcg acacccgcaa ggacatcgcc gaggccgagc
    43441 agatgcttga ggcgcgtcgc caccgcatct tcaaactgaa aatcggcgcc ggtgaggtga
    43501 accaggatct ggcccacgtc atcgccatca agcaggccct gggtgagcgc gccagcgtgc
    43561 gtgtagacgt caaccaggcc tgggacgaag cggttgccct gcgcgcctgc cgcatccttg
    43621 gcgataacgg tatcgacctg atcgagcagc cgatctcgcg ccacaaccgt ggtggccagg
    43681 tgcgcctgaa tcagtccagc ccggcgccga tcatggctga cgaatccatc gagtgcgtgg
    43741 aagacgcctt taacctggcg cgcgacggcg cggccccggt gttcgccctg aaaatcgcca
    43801 agaacggcgg tccacgcgcc gttctgcgca ccgccagcat cgccgaggcg gccggcatcg
    43861 gcctgtatgg cggcaccatg ctcgagggca gcgtcggcac cctggcctcg gcccatgcgt
    43921 tcattaccct gagcaagctg acctgggaca ctgagctgtt cgggccgctg ctgctgaccg
    43981 aagacatcgt taccgagccg ccgctgtacc gtgatttcca cctgcatgtg ccgcgccttc
    44041 cgggcatcgg cctgaccctc gacgaggagc gcctggcgcg cttccgccgt ccctgaccct
    44101 gcccgaagga gagccatcat gctgttccac gtgaagatga ccgtgaagtt gccactcgac
    44161 atggatcccg cccaggcggc caaactcaag gccgacgaga aggcgctggc gcagcgcctg
    44221 caggacgagg gcaagtggcg tcacctgtgg cgcatcgccg gccattacgc caactacagc
    44281 gttttcgatc tgagcagcgt cgaggaactg catgacacgc tcatgcattt gccgctgttc
    44341 ccctacatgg agatcgaggt aaatggtctg tgccgccacc cgtcgtcgat ccatcaggat
    44401 gatcgctgat cagccatcca gccgataaaa acaagatgag gaaatagcca tgagtatcaa
    44461 actgtcgcat accgaaagcg tgcagagctt cttcaaggaa gtcagcggcc tcaacaacga
    44521 ccagggcagc ccgcgcatga aggcgctggt caaccgcatc ctgatggaca gcattcgcct
    44581 catcgaggat atgcaggtca ccaccgagga gttctggaag gcggtggact acctcaaccg
    44641 catgggcgcg cgcagcgaag ccggcctgct ggtcgccggt ctgggcctgg aacactatct
    44701 cgacctgctg ctcgatgccc aggacgaggc ggccggtctg ctcggcggca ccccgcgtac
    44761 catcgaaggc ccgctgtacg tggccggcgc accgctgtcc gaaggcgagg cgcggatgga
    44821 cgacggcacc gacccgggca cgccgatgta cctgcatggc caggtgctcg ataccgatgg
    44881 caagccggtc gctggtgccg tggtcgacct gtggcacgcc aacaccaaag gcacatactc
    44941 ctacttcgat tcgagccaga gcgagttcaa cctgcgccga cgtatcgtca ccgatgctga
    45001 aggccgctac cgcgcgcgca gcatcgtgcc gtccggctat ggctgcccgc ccgatggcac
    45061 cacccaggaa gtgctcaacc tgctcggccg ccacggcaac cgtccggcgc acatccactt
    45121 ctttatctcc gctcctggtt atcgtcacct gaccacgcag atcaacctgg ctggcgacga
    45181 atacctgtgg gacgacttcg cctatgccac ccgcgatggt ctgatcggcg acgtgacatt
    45241 catcgaggac gccgccgcgg cgaatggcca tggtttccaa ggcgcgcgtt tcgccgaggt
    45301 gaacttcgac tttcagttgc agggggctgc cttatcggac gccgaacagc gcaacccgcg
    45361 cccgcgtgcc ctgcaataaa tcgcgctcct gctctacccc tagccggccc attgaggccg
    45421 gtttttttta gggaaattca gaagaaggtg aacaattcac tttcgagggc cgccgacaga
    45481 gtcgttgtcg gttgctaacg agcggttcaa accgttcgct tcgctcactg ggatgggcta
    45541 aagcccgccc ttaaccaaac gttatgtttc tcacattctg taatgggcta ggtgtatgtt
    45601 aaaaatcgga cttgtttatt ttagtaatac cgatgtgacg ggtgagttga tgggcgctat
    45661 taatgaccag ttgttgggtc ttgggtgcag tgtcattaaa cacagaatta gcggtagtga
    45721 aatcatcaat ggacgttttg aacgccctgc gctttttcaa gaattacata gctgtcatgc
    45781 cataattttt ggctctccaa cctacatggg gggcgtttct gctcaattca aaacttttgc
    45841 agacgcaacg agtgattttt gggaaagcca aacatgggct ggcaagctag ccgctggtgt
    45901 cacgtcgggc accgggttaa atggcgatca aggtcatacg ctgagttata tgcagttgct
    45961 ggcaagtcag catgggatgt tgtgggtgag cttggatgct ccatttaacg atgccgaaaa
    46021 aggtgtcaat cgactgggtt gccatatggg cgtaaccgca cacagtacaa ccggtaaagt
    46081 gtgtgagtta gacataaaaa cagcaaatta tttggcaaat aaaatagtgg gctatgcaat
    46141 aaggttcagc gccgaaacat aacaagcacc taaactccgg caatgtaatt gttgtcatgg
    46201 ttttagcaag tatcgcaaac aagcaatacg tggtgcaccg ttttgagtcg agtccgaatg
    46261 ataatttcaa gagtgtgccg ggctgatgcg acgcgtcgtt cagtcgacgc atatccggac
    46321 tttcagtccg taagtcacta acccaccgac ttcagtcggt ggttgttgag tgggcagttc
    46381 tgatctggtc tttttacagc gatcgacagt cgtcgctggc gggcaccgta ggccgctgtt
    46441 gttggctttc tgaagcggca caagcgtgtc gtacgagtgt gtttagtagt ggcgcgataa
    46501 aagcgatcag tagcttccgt cacgttactt cctggccatc aaagtgtatg cggctgatcg
    46561 gttctagaag tactgcaaca gagcggagct gaatgcgcca ttacaaaaaa catgctcggt
    46621 tagatgccaa agagatacga cttcgaattc gtattacctg atttaggccg cttctttagt
    46681 ggtcgattat ggcccttaca ccattattca ttgctgcagc tcacgtctgt tgcggccagg
    46741 aaaacgctat gacagcccca atactaggca ttgatccgcg ggctttccgc tgcgcgctgg
    46801 gtaactttgc caccggtgtc accgtcatct cagaaggcgt cagtcagagg tgggtgagta
    46861 aagttcgttg accaggatat gggtgatttc agcctgggca cgggcatagt cttgttccat
    46921 agcgtattgc aatgtggttg cttgggtgcg ttcctgagcg atgacatcgt tccagaactg
    46981 agcgttcctc gcgggcggta cgacgtcttg cagttcagcg agatagtgat gcgtagcccg
    47041 cgaaagcctg gcagcgcggc atgcctttga ccagacctcg gtggttagta tcggcggcaa
    47101 agaaggcctg catagcgccc tgatgtggtt gaagtccgat aaccgcgctc aggcctcagg
    47161 ccagcaccga gctggctgcc gccaggctgg cctacgacag cgaataagtc ggcttggccc
    47221 tgagctacat tcgtggtctc gatgtgtgtg agcgttatgc cagcgcgttc cagcaacagc
    47281 gtgacggtat ggatatctat gggtaacgcg ggagtggaga acgccaactt cgcctttgaa
    47341 tacgcctggc aggataggtc ggcgggtgat gagagggtct ggtatgccgt tgctcggctt
    47401 ctatcagccg agcaagagtc tgggcaatgg tgggcttcag ggcggcggta atggtaccaa
    47461 cgtgtacagc caactgactg tcgccttacc gttgtaatcc gcagccccac cagcctgtta
    47521 atggtggttg tcgggggcgc gatattgacg ccgccatagc gaggtctgcg cgtcgtctgg
    47581 ctttggcgta ggcaagatca gctccgaagc aagcagacgc ttatagctcg cccctcacat
    47641 gaaggggctg ggcttgagtc ttcgacatac tcagtcctga ccctatagat caagcatcga
    47701 actcggcgta gtgatcagct tggtatcgaa tggggccttg atatggcgac agatcctaat
    47761 cacctagatc gctattgatc aaagcaagag cgccggtagt tagttcatgt ttgagggcag
    47821 cgtaattaat tacgccgttt tcgctcgcgg gggtgacgag aacaacgatg ctgccacgga
    47881 tcatagtttc aggcctcgga ttgttgttct tttatcgggg cgcaaaaggc gggtggtgct
    47941 ttcgccgcgc ggctgtgcag ccactataga aagcacgcaa gacagtgcct tagcccaaat
    48001 cggcagtgtc cttgcacacc ttgccgggag ggctgtgcct catgttcggg cacggctacc
    48061 gatgctgccg atttgggcta acgcctcgtt ggggatgggc ctaggatcga agtcgctagc
    48121 gtgactacaa caattaattc gctggttagc atgagtggca gttggctgct ctgcaaccga
    48181 tccacgactg cctcatacaa caaggtcaag aaaatgagcg tacccatgaa tccggccgtc
    48241 agctccaatg agctgcggcg tggcgccctc agcgtcggtt ttatcatctt ctttgtggtt
    48301 tctgcggcga gcccgttaag cgttttggcg ggcggttttc ccattggcat catgctcggc
    48361 aacggtgccg ggacacccgt attactcggt ttgacactgc tggtgctgct ggccttctcg
    48421 gtgggctaca ccaccatggc ccgccatgtc accaatgccg gcggcttcta tgcgttctcg
    48481 gcgcgcggtt tgggtggtct ggcaggtggc gcggccgggg tgctggcgat gtttgcctat
    48541 aacatcctgc aggtgggcct gtacggcatg ttcggtggcg tcgctgccgg caccatggcc
    48601 agcgtgttcg ccatcgacct gccgtggtgg gtctattcgc ttcttgcgat ggccagtatc
    48661 gccattctgg gttaccgcaa tatcgacctg tcggcccggg tgctctcgtt cattgtcgtc
    48721 gcggagtacc tggcgattct caccctggat atcgccatcc tgaaaagtgg cggtgacagc
    48781 ggcattaatc tcgattcctt caagcctgag catgtattca gcggtaatcc gtcgataggt
    48841 ctgctgttct gctttgccgc tttcatcggg ttcgaggcca cgaccattta tggcgaggaa
    48901 gcgaaggacc cgcagcgcgc gattcccttt gccacttacg cgtcggtgct gttgatcggt
    48961 tgtttctatg gcctgtcgat ctgggcgatg gtggtcggtg tcggttccga caagatcgtg
    49021 ccgctgctgc aatcgctgga cgatccgacc accttcatct atggcctgtc cgatcacttt
    49081 gtcggtcccg agctgaccca gttaatccgc gtgctgtttt tgatcagtat ctatgccggt
    49141 ctgctcgcgt tccacaactc ggccgcgcgc tatttctacg ccactggccg cgatggtctg
    49201 ttgcatggcc tgctgggtac aactcaccgg attcatcaga gcccgcacat gggctcagct
    49261 ctgcaaaccc ttattgcggc tgtggttgtg ttgatttttg ctgctgccgg tgccgacccg
    49321 atcctgcaac tgttcgcctg gttctccagc ctggctgcgc tctcgctgat cctgctgatg
    49381 gcgctgacat cgatcgcggt gctggcctac ttccgcaagc atcctgagct tggtctgagc
    49441 ctcacgcgtc gcttcattct gccggccttt tcgggcatcg cattgatctg tgtactggta
    49501 cttgcagtga tccatttcga ggtgctgacc ggctccagca agctgctctc ttacgggctt
    49561 tgcatcctga ttccgatttc acttttcgtc ggtgtgatgc tcgccgcgcg tctgcgccgt
    49621 gtgtcgcccg aacgcttcca ggccctgggc agccacaaac tctaagtggt tgccaggcat
    49681 tcaccacctc tacaccagct aaccgccgtg cacgttgtgt ggccggcggc caaggggatg
    49741 cttcatatga acgactatca gatgcttatc gacggtgccc aggttgcggg tgagggcgag
    49801 ccttttgagg tgatcaaccc cgccaccggg caggctttcg cgctttgtcc ggccggcagt
    49861 gtggcgcagc tggagcaggc tgtggaggcc gctcagcgtg ccttccgcag ttggggccgc
    49921 acctcgtgcg tagaacgttg cgagcgcctg acacgcattg ccgatgacat cgagcgagag
    49981 gccgaagtgc tggctcgact cattgtcatg gagcagggca agccgctggc gctggccttc
    50041 tccgaagtca tgggcggtgt tgcctggacg cgttatgccg cctctcaaga actgccggtg
    50101 gagctgatcg aggaaacctc cacccaacgc attgaactgc accgcaagcc attgggcgtg
    50161 gtggcctcga tcacgccgtg gaactggccg ttcatgatcg ccatctggca catcatgccg
    50221 gctctgcgag cgggtaactg cgtgatcagc aagccgtcca gcctgacccc gctgagcact
    50281 attcgcctgg tggatatcat cgcccgtcat gtaccggctg gtgtgatcaa catcgtcacg
    50341 ggcgagcgtg gctttggcag cgccatcgcc agccatccgc gcatcgacaa aatcgtcttc
    50401 accggctcca ctgaaaccgg tcagcgcgtt atgcaggcgg ctgccggcaa cctcaagcgc
    50461 ctgaccctgg agctcggcgg caacgatgcc gcgatcatcc tgcccggtac atcggtcgag
    50521 gccgtagccg aggacatctt ccaggccgcc tttctcaaca tgggtcagac ctgcgccgcg
    50581 ctcaagcgtc tgtatgtgca cgaatcgcaa taccaggcct tcgccgaagc gctggctgcc
    50641 atcgcccggc gccaggtggt cggtgacggt atggaagtgg gcgtcacctt cggcccggtg
    50701 caaaaccccg agcagctgca actggtggct gagctggtgg aggacgcctg cgccaaaggc
    50761 ggccaggcgc tctgtggtgg tgcgccgctg ccgcgtgctg ggttcttcta cccaccgacc
    50821 attgtcaccg gcgtcagcga cggccaccgc ctggtggacg aggagcaatt cggcccggta
    50881 ctgccgctga tcgcctaccg cgacgtggag gacgcattgc gccgcgccaa cgccagcacc
    50941 atgggcctcg gtggctcggt gtggggtcca gatacggccg aggcacaagc ggttgccgcg
    51001 cgcctggaaa gcggcgtggc ctgggtcaac tgccacgccc agatccaacc caataccccg
    51061 ttcggcggct gcaaaatgtc cggtttcggc gtcgagttcg gccaggaggg gctgttggaa
    51121 ttcaccaccc agcagctgtt gttcgtacgc aagccaacgg ctccgcagtg aggaccaaca
    51181 gcatgtcgac ccataccgtg ctcaagcaca ccgaaacccc agatgcagcg cagggccaag
    51241 ccatgtacgc aaaatacaaa acagcagaga agaaattttg gcacccgatg agcgcctcag
    51301 cgccggccaa ccgtgccaat accctgatca tcgcccgcgg tgatggcaac tatatcaccg
    51361 acattgacgg taaccgcctg ctcgatggcg tcggtggctt gtggaacgtc aacgtgggcc
    51421 acaatcgctc cgaagtgaag gccgccatcg ctgcgcagct cgacgagttg gcctactacc
    51481 aaaccttcga cggtattgcc cacccgcgtg tgttcgatct ggccgagcgc ctgacctcga
    51541 tgttcgccca ggagaaaatg gcccgcgtgc tgttcaactc cggcggctcc gatgctgtgg
    51601 aaactgcact gaagatggcg cgccagtatt ggatcgcctc tggccagcct ggccgtaccc
    51661 gtttcttgtc actgcgcaac ggttaccacg gcgtacatat cggcggcacc tcaattggtg
    51721 gcaacggcgt ttaccactac aaccacggtc aactgcttgc tggttgccac ctgctcgata
    51781 cgccttggct gtatcgcaat ccctgggatt gccgcgatca agaggaactc gtcgaacatt
    51841 gcatccgcca actggaagac cagatcgcct tcctcggcgc cggcaccatc gcagctttca
    51901 tcgccgagcc ggtgcagggt gcaggcggcg tgatcgtacc gccgtccgcc tactggccgc
    51961 gcctgcgcga agtctgtgac cgccacgata tcctgctgat cgctgacgaa gtggttaccg
    52021 gcttcggccg cagcggctgc atgctcggca gccgtggctg gggcgtggcg ccggacatcc
    52081 tgtgtctggc caagggtatc tccgccggct acatcccgct gggagccacg gtgttcaacc
    52141 agcgcatcgt cgatgccatc gaaaacgccc ccggcttcgc caacgtgatc atgcacggct
    52201 acacctacag cggccatccg accgcctgtg cagcagccct ggcagtgttg gacattgttg
    52261 agaaggaaga cctgccaggt aacgcggcga aggtcggtgc gcacctgatc gacggcttga
    52321 cgcccctgag cgaacgtttc gatgtggtgg gtgaagtacg tggcaagggc ctgatgatcg
    52381 ccctcgatct ggtcgccgac aaacgcaccc gccagccact cgacccggct tcggggcagg
    52441 cctcgcgcat cgctgaagcc gcgcgtgcac agggcgtgct ggttcgtccg gtgggcaaca
    52501 agatcatcat ctcgccgccc ctgactttga ccaccggcga agccgacctg atcgtcgatg
    52561 tgctgcacaa cgccctgacc aagcattgaa cctgccagcc cgcggcttgc gccgcgggca
    52621 tccataccct cccgccaaac caataagaac gactgcacaa gcaccgagga gctcaatgaa
    52681 gatgaacgca cgacacaaca gcaagatcgc cctggcattg gccgtcaccg cggccctgtc
    52741 tgggcagacc caggcgtacg agctgtacgc cgatgaagac acccaactga atgcggatat
    52801 gctcgcggta ttcggctgga tgaacagccg taagaactac gatggcacgc cgggtggttc
    52861 gagctggcgc gagggtttca tcaagtacgg gctcagcggc cagcaggggt taggcgaggc
    52921 cggcagtctt tatggcgcct tcaatctggt cagttccggt acctggggtg acggtgatgc
    52981 aggcggcaat agcgttggtt cagagcgcac caccaagatc gaagatgcct atctcggctg
    53041 gcgttctgca gacctgttcc ctgtgctggg caaggatggc cttgatgtct cctttggtcg
    53101 tcaggtcgtg caactgggca aggggtttct gatcaacgac gacggcccaa acctgggcaa
    53161 aggcccggcc gatggcgcgc tcaaccgcgg tggtgcctat tacctggcag cacgccatgc
    53221 cttcgaccgt accgccgtgg tgcgcatcgg tggccaggac ggcctgcaca gtactctggc
    53281 ctggctgaag tccgacaacc gggcacaggc ggaaactgaa ctgacggctg ccacgctgga
    53341 ctatcgcggc gaagcagggc tgctgggact gacctggatt cacggtcttg atgtcagcga
    53401 tgaatgggcc gacgatttcc tgcagcagcg tgacggcatg gacatctaca gcctgcgcgg
    53461 tgaaagcact gcgggcatcg agaacgccag cttcgccttt gaatatgcct ggcagaacaa
    53521 agacgccggc gatgagaaag cctggtacgc cgaagccggc tacacctttg ctgatgcgcc
    53581 ctggtcaccg agcctgacct atcgctatac ccgctactcc gaaagctggg acgcgctatt
    53641 caccggcctc aacagcccgg agctgggtac ctggtttcag ggtgaggtgg cggccaacta
    53701 tgcagggctt tttgcgcgca acaatgatgt gcaccacctg gctttcaaag ttcgcccgct
    53761 ggagaatctg accctcggtg cgctgttctt cgacttccgc accctgcacg agcgtgacac
    53821 ggtcaaccgt gatgcccgag agctggattt gtatgcggaa tgggtggtca atgaccactt
    53881 gatcatcacg ccgctggtgg gcctgttcaa accggaccgt agcgacagta atggcggtac
    53941 ccagttcggc ggtaatggca caaacgtcta cagccaactg gttgtcgccg tgccgttctg
    54001 atttcagcgt taccctcttg gcgtgaccag gctttgctcc taaccacgct tctgccgcgt
    54061 gtcacgcagc cgccttcggg cggctgtttt tatggctgca acggccaact cactacgaag
    54121 cggctgccgc cattggcaga cggctggcaa taagcctgcc cgccatgcgc ctcggcgatg
    54181 gccttgacca ccgccaggcc gaggccgctg ccgccgctct tgcgtgagcg cgaagggtcg
    54241 ccacggcgaa aagcctcgaa gatgtcggcg gtcagggcgc tgtcgatacc agggccgtcg
    54301 tctgccacgc tcagttggca gtcgccgccg acctgttcca ggcacacccg tagattgcct
    54361 gggtcggcat ggcgctgggc gttttccagc agcgccatca gcgcctggcg gatgcgcacg
    54421 gcatcgcact ccaccagcag ctcggcgtcc agttcctggt gtaggtgaaa acccttttcc
    54481 gccaggggcg tggcgaaagc ctgcagcacg gtgcccagtt cattggcgag gcgtacgcta
    54541 ctccgttgca cttgcagatg gccgctgtcg ttgaggctca gcacgcgcag gtcttcgatc
    54601 aggtggttga ggccctccac ctggcgtagc aggccttcga acagctcgct gctggggctg
    54661 aacacgcctt cagccaaccc ctgcaaacgg ccgcgcagaa tggtcaccgg ggtgcgcagt
    54721 tcatgggcga tggcagcgtt ccagaactcg cgctcgcggg tcatgctttg caggcgttcg
    54781 gccatggtgt tgaagtcgag taccagttgc gcggtttcac ccatggcctg tgcgtccagc
    54841 ggcgcacggg cgtcgagctt gccctgagca acttcgcgca ggctgtgcgc caccgagttc
    54901 agcggcgtga tcaggcgccg tgacaggcgt atggcgacga agacggccat gctcagcgcg
    54961 gcgatgatgg tgcagacgat ccagaccagc tccagctgtg agggcaccat ggtgtcggaa
    55021 atgctgcctg gcaggtaggt cacagcgaag gcgtagaaca cgtaggtgcc gatcaccgag
    55081 aggcagatga tgcccagcgc catgaccgcc agggaatgga tgatctgtcg gcgcaggcct
    55141 ggcgtcggtt tcatgactcg acgccgaagc gatagcccac gccccagacg ctggtcggca
    55201 cgccctggat atctagcgct tcgagtttct tgcgcagttt gctgacgtgg ctgtccacgg
    55261 tgcgttcttg ggtgtcgccc tcgggcaaac agttttccag cagttcggcg cggctgaacg
    55321 cccgttttgg cgcgcgcatc aggcaggcga gtagcttgaa ttcggtcagg gtcagttcca
    55381 gcgtgtgctg ctggccatca cgctgcacgc tggcctcgtg gctgtccagg tcgatctgga
    55441 atgcgccgac gcgcagtacc tgcgtagcgg ccggggcgct gccccgggta cgccgtagta
    55501 ccgcctgtac ccgcgccacc acctcggccg ggttgaaggg tttgaccacg tagtcgtcgg
    55561 cgcccaggcg cagccccatc agcttgtcga tgtcctgatc cagcgccgtg agcatgatca
    55621 ctggggtgtc gccacgggag cggatttcgc tgagcacctt ccagccatcg aacacgggca
    55681 tctgcacgtc gagcagcagc aaatccggtt tgagcgtctg gtgcagggcc agcgcctgct
    55741 ggccgtcgct ggcgtgctga gtgcgcaggc cgctgcgctg caggtaggca atgagaatgt
    55801 cggcgatctc ggcttcgtcc tcggcgatca gcaccagtgc cgtagcgggg gaggcggggc
    55861 tggtgcgcgc gtaggaatcg gacatgggca ggctcgcaga cggtggcgct gaatgttatc
    55921 gcgccctcca tgctttctcc acaaagcctc gaatcattcg caacagcgct attccaaact
    55981 gcagcctcga tcagcgccag gtcgccatca ggtcaccgga gcgcagtggg caacgaggag
    56041 tgggttatgg gcagcaaaca gcggcatctg ggcatcgtgc tcgcagcgct tgccgtgttg
    56101 ggattggcag ggtgcgatgc ggcgttagag caagccgaag cgactgcgcc gctgcaggtg
    56161 tcggtgctga cactgcaggc gcacgaactg gcggtcagcg aggacctgtc ggcgcgtgta
    56221 gttgccgtgc gtagcgccga gattcgtgcg cagatcggcg gcatcgtgca gcgccgtctg
    56281 ttcgagcagg gcgccgagat caaggccggt gccgtgctgt tccagatcaa tccggcgccg
    56341 ttcaaggccg aggtcgacag cgccgctgcc gccttgcagc gcgccgaagc ggtgctggcg
    56401 cgggcgcgtg tgcaggtcga gcgcctgacc tctctggtgc aggccgaggc cgtcagccgc
    56461 caggtgtatg acgatgccgt ggccctgcgc gaacaagccg tagccgacgt ggcccaggcc
    56521 aaggcgaccc tggcgcggcg caagctggac ttgcagttcg ccagcgtcga ggcgccgatt
    56581 gcgggtcgta tcgaccaggc gctggtcagc gaaggtgcgt tggtatcgcc gaccgatgct
    56641 acgccaatgg caaggattca gcagatcgac caggtgtacg tcgacgtgcg ccaatccgcc
    56701 tcgatactgg acacgctgcg tcagcaggcc ggctcggcga cgccggaact ggacgtggaa
    56761 atccttggca gcgacggcaa gccgctggac atgcgcgggc gcatcctgtt ctccgggatc
    56821 gaggtagatg ccggcaccgg cgatgtactg ctgcgcatgc tggtggacaa cccgcaacgc
    56881 cgcttgctgc ccggcctcta cgtgcaggca cggattcccc gcgcgcatta ccccgccgcc
    56941 ctcagcgtgc cgcagcaggc cgtgacccgc agcgcaggcc aggccagcgt atgggtggtc
    57001 gatgcccagg gccaggtgca ggcggtgccc attgaggtcg gtgagctggt cgagcgccgt
    57061 taccgcgtgg tgtccgggct cagtgccggg caacaggtgg tggtcgaggg catcgagcgg
    57121 ctgagccctg acacccagct cgccacgcgc ccttggcagc ccgctgtcac cagcgaacag
    57181 ctcagcgccg tcgcgcgctg agttcggaga aatcgtcatg ccgcagtttt ttatcaaccg
    57241 cccagtgttt gcctgggtga tcgctctgtt catcgtgctg gtcggcgtca tcgccattcc
    57301 acagttgccc attgcccgct atccctcagt agcaccgccg tcggtaacca tctacgccac
    57361 ctatccgggt gccactccgc agacgctcaa cgactcggtg gtaagcctgg tcgagcgcga
    57421 gctgtcgggg gtgaagaacc tgctgtactt cgagtcctcg gtggacacct cgggcaccgc
    57481 gcagatcacc gccaccttca agccgggcac cgatgcggag ctggcccagg tggacgtgca
    57541 gaaccgtatc aaggcggtgg agccgcgcct gccccaggcc gtgcggcaga acggcctgca
    57601 agtggagtcc gccgcctcgg gctttctgat gatggtcggc atgacctcgc ccaacggcca
    57661 gttcaatgaa gtcgcattaa acgactactt ggcgcgcaat atcgtccagg agctacggcg
    57721 catcgacggc gtggggcgcg tgcagctgtt cggtgccgag caggccatgc gtgtctgggt
    57781 cgacccgaac aagctcaccg cctatggcct gaccatgaat gaactggccc aggccatcga
    57841 acagcagaac cagtcgattg cccccgggcg gatcggcgac gagccagcgc tgccaggcca
    57901 gcgcctgacc gtgccgctga ccgtgcaggg gcaactgagc acgccagagc agttcgctgc
    57961 cattgtcctg cgtgccgctg ccgatggcgc caaggtggtg ctcggtgatg tggcgcgggt
    58021 cgagctgggc gcgcagtcct acggattctc caaccgtgaa aacggcgtgg cggcgaccag
    58081 tgccgccatc cagctgtcgt ccggcgccaa cgccgtgcgt accgcctcgg ccgtgcgcga
    58141 gcgcctggcc gagctggcac cgagcatgcc tgccggcatg gcctactcgg tgccgttcga
    58201 caccgcgccc ttcgtcaagg tgtccatcaa gaaggtgatc tacaccctgt tcgaggccat
    58261 ggtgctggtg tttctggtga tgtttctgtt cctgcagaac ctgcgctaca cactgatccc
    58321 ggccatcgtc gcgcccatcg cgctgctcgg caccttcgcg gtgatgctgc tggcgggttt
    58381 ctcgatcaac tcgctgacca tgttcggcat ggtgttggcc atcggcatca tcgtcgacga
    58441 cgccatcgtg gtggtggaga acgtcgagcg gctgatggcc acccaggggc tgtcgccgaa
    58501 agaggcgacc aagcaggcaa tgcgggaaat caccggcgcg gtgatcggca tcaccctggt
    58561 actgactgcc gtgtttatcc ccatggcctt cggcagcggc tcggtggggg tgatctacca
    58621 gcagttcacc ctgtcgatgg cggtgtcgat cctgttctcg gccttcctcg ccctgaccct
    58681 gacgccggcg ctgtgcgcca cgttgctgcg cccggtaagc gcggagcatc atgacaaacg
    58741 cggcttcttc ggtgccttca accgcggctt cgagcgcctc acggcgagct atggggcgcg
    58801 ggtggcgcgc ctggtcgggc gcagcgggcg gatgatggcg gcgttcgccg tgctctgcgc
    58861 ggtactggcc gtcgctgctg cgcaattgcc gtcgtccttc ttgcccgagg aggatcaggg
    58921 ctactttatg acctccatcc agttgcccac gggcgccacc agcgaacgca cgctggacgt
    58981 ggtcaaggcc tacgaggcgc acgtcgccac gcgtccgggg ttggacgcca acttggtggt
    59041 gctgggcttc agcttcgccg gctccggccc caacgcggcc atggccttca ccatgctcaa
    59101 ggactgggat cagcgcaatg gcgccaccgc cgccgaagaa gccgcgctgg cgcaacaggc
    59161 catggccgac aatgtcgaag gcacggtgat gagcctgatg ccgccggcca ttgacgaact
    59221 gggcacatcc tccggtttca ccctgttcct gcaggatcgc gccaaccggg gcgaggccgc
    59281 gctgctggcc gcgcaagcgc aactgctggg gctggcggcg cagagcgagg tggtcagcga
    59341 tgtctatccc gatggcctgc cgcccggcga gagcattcgc ctggagatcg accggcagaa
    59401 ggccgaggcc atgggcctgt ccttcgcggc ggtgagcagc acgctgtcgg cggccatggg
    59461 ctcgctgtac gtcaacgact tccccaatgc cgggcgcatg cagcaggtca tcctgcaggc
    59521 cgacgccccg gcgcgcatgc agctcgacga cgtgctcggc ctgcgcgtgc gcaatgctgg
    59581 tggtggcatg gtggcgttgc gcgaggtggt gacgccagtg tggagcgagt cgccgcagca
    59641 gatgatgcgc ttccagggct tccccgccgt gcgactcgcc ggcggcgccg cgccgggcgt
    59701 atccagcggt gcggcgatgg ccgagatgga gcgcctggtg acgcagttgc cgcaaggctt
    59761 cgctgtggcc tggacgggac aatcgttgca ggagcgccag tcggcagcac aggcgccgat
    59821 acttatgctg ctctcggcac tggtggtgtt tctggtgttg gcggcgctgt acgagagctg
    59881 gtcgatcccg ctgtcggtga tgctggtggt gcccctgggc ctgctcgggg cggtggccgc
    59941 ggtgatgctg cgcggtatgc ccaacgacgt gttctttaag gtggggatga tcaccatcat
    60001 cggcctgtcg gcgaagaacg ccatccttat cgtcgagttc gcccgtcagt tgcacgccga
    60061 gggccgcacg ctggtcgatg ctgcggtaac cgctgcgcgc ctgcgcctgc ggccgatcct
    60121 gatgacctcg ctggccttca ccctcggtgt ggtgccgctg atgctcgcct ccggtgccag
    60181 cgccgaaacc cagcacgcca ttggcaccgg ggtgttcggc ggcatgctca gcggcaccct
    60241 gctggcggtg ttcttcgtgc cggctttctt cgtcttcgtt atcggtacgc aggagcgttt
    60301 cagcgcctgg cgtgcgcagc gtggcgccac cgcgtcacct caggagaacc cgtgatgcgt
    60361 ctgttcctga ccctgccgct gcttgcctgc ctgagcgcct gctcgctgac tccgaccctg
    60421 gagcgcccag ccgcaccagt gccagcgagc tttgccatgg ccgccacccc acctgcttcg
    60481 acgttgccgg ccagcgcatt gggctggcgt gcgatgttcg ccgacccgcg cctgcaacgg
    60541 ctgatcgacc tggcactggc taataaccgc gacctgcgcc tggccaccct aaacgtcgag
    60601 gcggcgcagg cccaggcgaa catccagcgc gccgcacgtc taccggctgt ggggctggag
    60661 ggaacgcgca cgcgtgagcg cgccttgacg accgacgcga acggtcaggc cagccgcgag
    60721 gtgagccagc agaccggcgt gaacgtcgcc ctcagcgcct tcgagctgga cctgttcggc
    60781 cgcgtgcgcg ctctctccga tgccgcgttc gcccgctatc tggccagcga gcagggccgt
    60841 gatgcggcgc agatcgccct ggtcggtgcg gtggccgagg cctacttcgc ccagcgtctg
    60901 gccgatgagc aactgcagct caccgagcgc accctggccg actggcagca gtcgctggag
    60961 ctggcacgcc tgctcaagca ggccgagcag agcagcagcc tggatgtggc acaggccgaa
    61021 gggcaggtgg cccaggctga agctgacctg gaagcacgcc gccgcgccct ggcgcaggcg
    61081 aacaatgccc tgcagttgct ggtgggtagc gagctgccgg gggatctgcc tgtcggcttg
    61141 ccactggagc gtcagtcgct gatcgcgcaa ctgccggcgg gcttgccggc cgacctgctg
    61201 ctgagccggc cggacctgcg tcaggccgaa cagatgctgg tggccgccaa cgccgatatc
    61261 ggcgcggcac gggcggcgtt ctttccgcag atatccctga ctgcttcctt cggttatgcc
    61321 agcacctcgc tggctggcct gttcgacccg gcccggcaga tctggcactt cgccccgcag
    61381 atttcccagc cgctgttcca gggcgggcgc ctgcgcgccg agctacgcct agccgaggtg
    61441 cgcaaatccg aggcggtcgc ccagtacgag cgcgccatcc agacggcctt ccgcgaagtg
    61501 gtcgacgccc tcgcggggac ggccaccttc gaccgccaga tcaaggcaca actgcgcgcg
    61561 gtgacagcga ccgagcggcg tctgcaattg tctgacctgc gctaccgcgc tggcttcgac
    61621 ggccgcctgg agctgctcga cgcgcaacgc caactgcagg ctgcacggca aacgctgctg
    61681 gagttgcgcc gcgcagaaat cgacaaccga gtggcgctgt ataagtcgct gggtggtggt
    61741 ctacatgcgc gcgatattcc ggagatagcc gctgtcacgg cggctcagta gagtccatcc
    61801 atcttgttca tgagcgtttc catgtgtgtt gtcattcccc gtcgttggct gatcctgctg
    61861 gctgtgatgc tggccttcct gccggtggtc atcgacatga ccatcctgca catcgccgtg
    61921 ccctcgttga ccctggcact cggcgcttcg gccaccgagg tgctgtggat catcgatatc
    61981 tatccgctgc tgatggcggg cctgctggtg cccatgggca ccctggccga ccgcatcggt
    62041 aaccgccgcg tgttgcttat cggtttggtc atcttcggct tcgcctcttt gctggccgcc
    62101 tttgcgccga cagcgaccct gcttattggc gcacgggtgc tgctggccct cggtggttcg
    62161 atgatcatgc cctgcgtgct gggcatcatc cgccgtacct tcgaggacgc cgacgagcgc
    62221 gccatggccc tgggcctttg gggcacggtc ggtgcagccg gcgcggcact cgggccgctt
    62281 atcggcgggg cgctgctgga gcacttctgg tggggctcgg ccttcctgat caacgtgccg
    62341 atcatgctgg tggtcgcgcc gctggtgttc ctgctgttgc cgcgcacgga ggacaccacg
    62401 cccggtacct gggcgctggt ccaggcgctg ctgttgatcg ccggcatact ggcagtggtg
    62461 tacggcatca aggccgctgt cggtacaacg caaccgctgg ctgttgcgct atcggtggca
    62521 atactcgggg cgctgctgct gacgatattc gtgcgcctgc aactgcgctc ggcgacaccc
    62581 atgctcgacc tgtcattgtt ctcgcgtccg gcgatcctgg ccggaatcat catggcggcg
    62641 gtggccatcg gcgcgttggc cggagtcgag ctgacgctgg cgcaggaact gcagtacgtg
    62701 ctgggcaaga caccgttgca ggccgggatt ttcatgattc cgatcatggc tgccgccgct
    62761 gtgggtggcc cgatagccgg ctacttatcg aatcagtttg gcttgcgcct ggtttccagc
    62821 ctatcgctgg cagtcgcatc cgcagcactg gccgctttag cgatgactga actgcacacg
    62881 cctggctgga ttgtgccaac gctactggga ttcctggggc ttaccttgag catcgggttg
    62941 acggcttcat cgattgccat catgggctcg gtagacgctg acaaaggtgg cgctgcgggg
    63001 gcgctggagg cgacggca
//
LOCUS       DASRUM010000203         8464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1933016, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000203 DASRUM010000000
VERSION     DASRUM010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933016"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="VFF22_13460"
     CDS             complement(<1..1215)
                     /locus_tag="VFF22_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HZX17853.1"
                     /translation="MTIQATEVGKRRTFAIISHPDAGKTTITEKLLLMGKAIAVAGTV
                     KSRKSDRHATSDWMEMEKQRGISITTSVMQFPYREHMINLLDTPGHEDFSEDTYRTLT
                     AVDSALMVLDGGKGVEPRTIALMDVCRLRDTPIVSFINKLDRDIRDPIELLDEIEAVL
                     KIKAAPITWPIGCYRDFKGVYHLADDYIIVYTPGHGHERTETKIIQHLDSDEARAHLG
                     DEYERFVEQLELVQGACHEFDQQEFLDGQLTPVFFGTALGNFGVDHVLDAVVDWAPKP
                     LARIANERTVEPVEEKFSGFVFKIQANMDPKHRDRIAFMRICSGKYSQGMKMRHVRTG
                     KDVRIGDALTFFSSEREMLVEAYAGDIIGLHNHGTIQIGDTFTEGEALGFTGIPHFAP
                     ELFRRVRLKDPLK"
     gene            1692..2855
                     /locus_tag="VFF22_13465"
     CDS             1692..2855
                     /locus_tag="VFF22_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HZX17854.1"
                     /translation="MPIKVEDYYAPATFQRMKAFADTQETPFVVIDTAIISQAYDDLR
                     AGFEFAKVYYAVKANPAVEIIDLLKEKGSSFDIASIYELDKVMGRGVSPDRISYGNTI
                     KKSKDIRYFYEKGVRLFATDSEADLRNIAKAAPGAKVYVRILTEGSTTADWPLSRKFG
                     CQTDMAMDLLILARDLGLVPYGLSFHVGSQQRDISVWDAAIAKVKVIFERLKEEDGIE
                     LKLINMGGGFPANYITRTNSLETYAEEIIRFLKEDFGDDLPEIILEPGRSLIANAGIL
                     VSEVVLVARKSRTAVERWVYVDVGMFSGLIETMGEAIKFPLYTEKKGEMEEVVIAGPT
                     CDSADIMYENYKYGLPLNLAIGDRVYWLSTGAYTTSYSAVEFNGFPPLKSFYL"
     gene            3092..5365
                     /locus_tag="VFF22_13470"
     CDS             3092..5365
                     /locus_tag="VFF22_13470"
                     /inference="COORDINATES: protein motif:HMM:NF007349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HZX17855.1"
                     /translation="MSIRSRKHYKSSHLALLIAAAVPGMAFAAQNGAAQEELSLPATT
                     VTSKAEVPYKASKSANSKLTQPLLDTPKTVQIIKKEVLREQGANSLMEALRNTPGITM
                     QLGENGNTSAGDTFMMRGFSTQNSTFVDGVRDLGAVSRDVFNLEQVEVVKGAAGSDIG
                     RGASSGYINLISKLPTLTNEVFATVGYGTADKSNLTADINQQLSDNSAFRLNLMRRDG
                     DMDGRDYVDNSSYGVAPSIAFGLDTDTRVYVYSQHVRQDNTPDGGIPTIGMKGFYSAD
                     AALNSGRKVDTENYYGAKGDSERIDGDMFTVKVEHDFNDDLTLRNLSRWGHSNNDRTL
                     TGINALATNSSADPANWTVARSRQVVDQENEILANQTSLSAAFDTFGLRNELAAGLEL
                     MSEKQLNRGYATAAQTIDGVAYPAVPVPPANLYDPSAHDGLGDPYKTGAHTDGETKTA
                     ALYVFDTLHLNEQWALNGGVRFEHFRTETDSVAIASGARVPGSGYESGDLTSWNTGVV
                     FKPLENGSIYLSYANSLTPPGSGNFNLAASSATSSSNEQVGLDPQETEHLELGTKWDL
                     LDERLALTLAAYRTENENQVSYDSFTATYFQEGKTRVDGVELGVVGQLNNFWQVSAGI
                     AHMETKQIDQASRNNAGVVSESTGVRWSPDLTATLWTSYTLGDLTLGGGARYVSEQDR
                     VVTSGTNLATQNMPSIPSYWVADAMAAYRVNDNVNLRVNLYNLFDEEYIESLNNSGAR
                     ARLGAPRSAMFTTELSF"
     gene            5442..6119
                     /locus_tag="VFF22_13475"
     CDS             5442..6119
                     /locus_tag="VFF22_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004757131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HZX17856.1"
                     /translation="MLLHIPQVLSKQEVTALRAELAAHAWVDGVQTSGAQAASLKQNL
                     QFPADSPAFASLAQRVADALARHPLFVSAVLPRHMLPPMFNCYSGGGRYGNHVDNALQ
                     RDRFSNDQVRTDVSTTVFLSEPDEYDGGELIVEDTYGEHEIKLEAGDAIIYPATSLHR
                     VEPVTRGARIASFLWTQSRVRDAWQRKMLFDLDMTILKLRHDLGDSDEVVSLTGTYHN
                     LLRQWAD"
     gene            6121..7242
                     /locus_tag="VFF22_13480"
     CDS             6121..7242
                     /locus_tag="VFF22_13480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HZX17857.1"
                     /translation="MALSEPLSAIPADLVAACDYQRYALERLDANALAYLQGGAADEL
                     TCQANLDAWREWAIVPRMLRDLRGGHTRCQLLGAELQHPIVLAPVAYQRMFHAEGELA
                     TALGAGVMGGAAVLSSFASTPLEEVADVAPGPLWFQLYWQGDGPETLALAQRAEAAGY
                     RALVLTVDAPVSGLRNREQRAGFTLPDGIAAVNIGKPPVVPQVPAGGSAIFDAMLAIA
                     PTWDEVRWLCRQTHLPVLLKGILHPADARLAMDAGAAGVIVSNHGGRVLDSQWPALKA
                     LPGIRGELGAEALVLVDGGIRRGTDVFKALALGADAVLIGRPYIHALATAGALGVAHL
                     VRLLREELEMAMALSGCRTLSEINADLLCRSNDWPCPPK"
     gene            complement(7284..>8464)
                     /locus_tag="VFF22_13485"
     CDS             complement(7284..>8464)
                     /locus_tag="VFF22_13485"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HZX17858.1"
                     /translation="APDNAARFERALLSQPVLPQLHFAILALGDSSYPQFCAFGQRLQ
                     QRLQALKAQPLFDLLCVDNLDAGTLRHWQQQLGHLSGNSDFVDWQPAHYRPWQLAQRN
                     CLNPGSIGAPIYQLRLSSKEDVDWRAGDIVEVGPRHAPQRVIDCLQQLGLEPHTRLAN
                     DQLLSDALSTRHLPTTRPEKNAIEQWLAELTPLPHREYSIASSHGSGQLDLLVRLQYQ
                     ADGSTGLGSGWLCQHAEVGSQIDLRIRRNPGFHGPDAATPLILIGSGSGLAGLRAHLQ
                     ERAAVPHTRNWLVFGERNAAHDALLIDELRAWQHSGHLARLDLTYSRDGHPQRYVQDA
                     LRDAANTLHAWLADGAAIYLCGSLEGMGHDVDACLRQLVGDEQLEQLSQSGRYRRDLY
                     "
BASE COUNT         1635 a         2420 c         2650 g         1759 t
ORIGIN      
        1 tttcagcggg tccttcaggc gcacgcggcg gaacagttcc ggggcgaagt gcgggatacc
       61 ggtaaagccc agcgcttcgc cttcggtgaa ggtgtcgccg atctggatgg tgccgtggtt
      121 gtgcaggccg atgatgtcgc cagcataggc ctcgaccagc atctcgcgct cgctggagaa
      181 gaaggtcagg gcgtcaccga tgcgcacatc cttaccggta cgcacatggc gcatcttcat
      241 accctggctg tatttgcccg agcagatacg cataaaggcg atgcggtcgc ggtgcttggg
      301 gtccatgttc gcctggatct tgaacacgaa gccgctgaac ttttcttcca ccggctccac
      361 ggtgcgctcg ttggcgatgc gggccagcgg cttgggtgcc cagtcgacta ccgcgtcgag
      421 tacatggtcg acgccgaagt tgcccagtgc ggtgccaaag aacaccgggg tcagttggcc
      481 gtcgaggaac tcctgctggt cgaactcgtg gcaagcaccc tgcaccagct ccagctgctc
      541 gacaaaacgc tcgtactcgt cgcccaggtg ggcgcgggct tcatcggagt cgaggtgctg
      601 gatgattttg gtttcggtgc gctcgtggcc gtggcccggg gtgtagacaa tgatgtagtc
      661 gtcggccagg tggtacacac ccttgaagtc gcggtagcag ccaatcggcc aggtgatcgg
      721 cgcggccttg attttcagta ccgcttcgat ctcgtcgagc agttcaatcg ggtcgcggat
      781 atcgcggtcg agcttgttga taaagctgac gatgggcgtg tcgcgcaagc ggcagacatc
      841 catcaaggcg atggtgcgtg gctcaacgcc tttaccgccg tcgagcacca tcagtgcgct
      901 gtccacggcg gtcagggtgc ggtaggtgtc ttccgagaag tcttcgtggc cgggggtgtc
      961 gagcaggttg atcatgtgct cgcgatacgg gaactgcatc accgaggtgg tgatggagat
     1021 gccgcgctgc ttctccattt ccatccagtc ggaggtggca tggcggtcgg acttacgcga
     1081 cttaaccgta ccggccacgg cgattgcctt gcccatcagc agcagcttct cggtaatggt
     1141 ggttttaccg gcgtctgggt gggaaatgat ggcgaatgta cggcgtttgc cgacttcggt
     1201 ggcctggatg gtcattgtgc gggaacccgt cgactgaata gtcggtctgt caaaaaaggc
     1261 ggcgattata acggtaaacg tcggtcagcg cggcgacctg tagtcagtgc gcaaaacctg
     1321 ggttttttcg cgcgctgtgc agcatttcat ggcgttttgt tttgaatata aaacgttcaa
     1381 tcaataaggc gacaaagagg agcaaaagca cggcattttt gattgatctc agccccccat
     1441 ggccctaaag ttcgcgcccg aacgtccatg ctggcaacga tccatccggc tcaagtactg
     1501 acgacgagag gcccacaggg ttctcggcga catgccttgg gaagtaggcg aaccaaagtg
     1561 gggaaactga tcagacgttc tacctgtgcg ggctagccgc caggtctccc taaatggtgg
     1621 gcaacggact gcatacgcag cgtttgcctg actaaaactg tgttaacggg ttcagccaac
     1681 tggagttccg catgccgatc aaggtcgaag actattacgc acccgctacg tttcagcgca
     1741 tgaaggcgtt tgccgatact caggaaaccc cgttcgtggt catcgatacc gcgatcatca
     1801 gccaggctta cgatgacctg cgcgctggtt tcgaattcgc caaggtgtat tacgcagtca
     1861 aggccaaccc ggcggtcgag attatcgacc tgctcaagga aaagggctcc agcttcgata
     1921 tcgcctcgat ttatgagctg gacaaggtca tgggccgtgg cgtcagcccg gatcgcatca
     1981 gctacggcaa caccatcaag aaatccaagg acatccgcta cttctatgag aagggcgtgc
     2041 gcctgttcgc caccgactcg gaagccgacc tgcgcaatat cgccaaggct gcgccgggtg
     2101 cgaaagtcta tgtgcgcatc ctcaccgaag gctcgaccac ggccgattgg ccgctgtcgc
     2161 gcaagttcgg ctgccagact gacatggcca tggatctgct gatcctcgcc cgtgacctcg
     2221 gcctggtgcc ttacggcctg tctttccatg tcggctcgca gcagcgcgac atctcggtgt
     2281 gggacgcggc gattgctaag gtcaaggtga tcttcgagcg tcttaaagaa gaagacggca
     2341 ttgaactcaa gctgatcaac atgggcggcg gcttcccggc caactacatc acccgtacca
     2401 acagccttga aacctatgcc gaggaaatca tccgtttcct caaggaagac ttcggtgatg
     2461 acctgccgga aatcatcctt gagccgggcc gctcgctgat cgccaacgca ggcattctgg
     2521 tcagcgaggt ggtgctggtg gcgcgcaagt cgcgtaccgc ggtcgagcgc tgggtctatg
     2581 tcgatgtggg catgttcagt ggcctgatcg aaaccatggg cgaggcgatc aagttccccc
     2641 tctacaccga gaagaagggc gagatggaag aagtggtgat cgccgggccg acctgcgaca
     2701 gcgctgacat catgtacgag aactacaagt acggcctgcc gctcaacctg gccattggtg
     2761 accgcgtcta ctggctgtcc accggtgctt acaccaccag ttacagcgcc gtggagttca
     2821 acggcttccc gccgctgaag tcgttctacc tgtaacacct cctggttttc cggtcaaaac
     2881 cccgcctagg cggggttttt tatgcctgct ctttatctag aagcatcact ttggtgtggt
     2941 tgaaggttgg cgtgacagct gttaatgcaa atagttctta atatgcgttg ctgcaattga
     3001 tacaaataat tatcatccgc gcactttgag ttcctgcctc tcgccaacaa ccacatagag
     3061 agtcggggcg actttaaaac ggggagtaag catgtccatt cgtagccgca agcattacaa
     3121 gagcagtcat ctggcgctgt tgatcgccgc cgcagttccc ggtatggcat ttgctgccca
     3181 gaatggcgct gcgcaagaag agctgagcct gccggctacc actgtgacct ccaaagctga
     3241 ggtgccctac aaagccagca agagcgctaa cagcaagctg acccagccac tgttggatac
     3301 accgaaaacc gttcagatca tcaagaagga agtgctgcgt gagcagggcg ccaacagcct
     3361 gatggaagcg ctgcgcaata ctcccggcat caccatgcag ctgggtgaga acggtaatac
     3421 ctcggccggc gacaccttta tgatgcgcgg cttctccacg cagaactcga cctttgtcga
     3481 cggtgtgcgt gacctgggtg cggtcagtcg tgatgtgttc aaccttgagc aggttgaagt
     3541 ggtgaaaggc gctgctggtt cagatattgg tcgcggtgca tcgtccggct atatcaacct
     3601 gatttccaag ctgccgaccc tgactaacga ggtcttcgcg accgtcggtt atggcaccgc
     3661 cgacaagagt aacctcaccg ctgatatcaa tcagcagttg agcgacaact cggccttccg
     3721 tttgaacctg atgcgccgtg atggcgatat ggacggccgc gactatgtcg acaacagcag
     3781 ctatggcgtg gcgccttcca ttgcctttgg tctggatacg gacactcgcg tctatgtcta
     3841 ttcccagcat gtacgccagg acaacacccc tgatggcggt attcccacca ttggcatgaa
     3901 aggtttctat agcgccgatg ctgcgctgaa ttccggccgt aaggtcgata ccgaaaatta
     3961 ctacggcgcc aagggcgact ccgagcgcat cgacggcgat atgttcaccg tcaaggtcga
     4021 gcacgacttc aatgacgacc tgaccctgcg taacctgtcg cgctgggggc acagcaacaa
     4081 cgaccgtacc cttaccggca tcaatgcctt ggccaccaat agctcggctg accccgcaaa
     4141 ttggaccgtg gctcgttcgc gtcaggtggt cgatcaggaa aacgaaatcc tcgctaacca
     4201 gaccagcttg agtgctgcgt tcgacacctt tggcctgcgt aacgaactgg ctgctggtct
     4261 tgagttgatg agcgagaaac agctcaaccg tggctatgcc accgctgccc aaaccatcga
     4321 cggtgtggcc tacccggctg taccggttcc gccggccaat ctctacgacc cgagtgcgca
     4381 cgatggcctt ggtgatccat acaagactgg tgcccacacc gacggcgaaa ccaagacagc
     4441 agcgctctat gtgttcgata ccctgcacct gaacgagcag tgggcactta acggcggtgt
     4501 acgctttgag cacttccgta cggagactga cagcgttgcc attgcatcgg gtgctcgtgt
     4561 accgggcagt ggctatgagt cgggtgatct gaccagttgg aatacgggcg tggtgttcaa
     4621 accgctggag aacggcagca tttacctgtc ctatgccaac tcgctgactc cgccgggtag
     4681 cggcaacttc aacctggccg ccagctccgc cacctcctcg tccaacgagc aggtcggcct
     4741 ggatccgcag gaaaccgaac acctcgaact gggcaccaag tgggatctgc tggatgagcg
     4801 cctggccctg actctggcgg cctaccgcac cgagaacgaa aaccaggtca gctacgattc
     4861 atttaccgcc acctatttcc aagaaggcaa aacccgcgtc gacggcgtcg agctgggtgt
     4921 agtaggccag ttgaacaact tctggcaggt gtcggcgggt attgcccata tggaaaccaa
     4981 gcagatcgac caggccagcc gcaataatgc gggcgtagtc agcgagagca ctggcgtgcg
     5041 ctggtcgcct gacctgaccg cgaccctgtg gacctcctat accctgggcg atctgaccct
     5101 gggtggcggc gcacgctatg tgtctgagca ggaccgagtt gtcacctccg gcactaacct
     5161 ggcgacccaa aacatgccat ccataccgtc ctactgggtg gcggatgcca tggccgctta
     5221 ccgtgttaat gacaacgtca acctgcgggt gaacctgtac aacctgtttg acgaggagta
     5281 catcgagtcg ttgaacaaca gcggcgcccg tgcccgtctc ggggcaccgc gctcggcgat
     5341 gtttaccact gagctcagct tctgatagaa caaccagccg tatctgaagc ctgcgcgcaa
     5401 gcgcgggctt tttgcattct gtattgatgg agaggatgtt gatgctgctg catatccccc
     5461 aggtgttgag taaacaggaa gttacagcgt tacgtgccga actggcggcc catgcctggg
     5521 tcgatggtgt gcaaacgtcc ggtgcgcagg cggcaagcct aaagcagaat ctgcagttcc
     5581 cggcggattc gccggccttc gccagcctgg cgcagcgtgt agccgatgcg ctggcgcgcc
     5641 acccgctgtt cgtttctgcg gtattgccgc ggcatatgtt gccaccgatg ttcaactgtt
     5701 acagcggcgg tggtcgctac ggcaaccatg tggacaacgc cctgcagcgc gaccgtttca
     5761 gcaacgatca ggtgcgcacc gatgtttcca ccacggtatt tctcagtgag ccggacgaat
     5821 acgacggcgg cgagctgatc gtcgaggaca cctatggtga gcacgaaatc aagctggaag
     5881 caggcgatgc cattatctac cccgcgacca gtctgcaccg tgttgaaccg gtgacccgtg
     5941 gcgcgcgtat cgcctccttt ctctggacgc aaagccgggt gcgcgatgcc tggcagcgca
     6001 aaatgctctt cgatctggac atgaccatcc tcaagttgcg ccacgatctg ggcgacagcg
     6061 atgaggtggt gagcctgacc ggcacctatc acaacctgtt gcggcagtgg gcggactgaa
     6121 atggccttga gcgaacccct gagcgcgatt ccagccgatc tggttgccgc ttgtgactat
     6181 cagcgttatg ccctggagcg cctggatgcc aatgcattgg cctacctgca aggcggcgca
     6241 gcggatgagc tgacctgtca ggccaatctg gatgcctggc gcgaatgggc gatagtgccg
     6301 cgcatgctac gtgacctgcg cggcggtcat acccgttgcc agttgcttgg cgctgagttg
     6361 cagcatccca tcgtgctggc gccggtggcc tatcagcgta tgtttcatgc cgagggcgag
     6421 ttggccacgg ccctgggcgc cggagttatg ggtggtgctg cggtgctcag ctcgttcgct
     6481 tccacgccat tggaagaggt tgccgatgtt gcgcctggcc cgctgtggtt tcagctgtat
     6541 tggcagggcg acggcccaga gactctggcc ttggcacaac gtgccgaagc ggcgggctat
     6601 cgcgctctgg tgctgacggt ggatgcgccg gtgtcggggc tgcgcaatcg cgaacagcgt
     6661 gcaggtttta ctctgccgga cggtatagcc gcagtaaata tcggcaaacc gccagttgtg
     6721 cctcaggtgc cggccggagg cagtgcgata ttcgacgcca tgctggccat cgcgcctacc
     6781 tgggatgagg tgcgctggct atgccggcag acccatttgc cggtgctgct taagggtatt
     6841 ctgcatccgg cggatgcgcg tttggccatg gacgcggggg cggcaggcgt cattgtttcc
     6901 aaccacggtg gccgggtgct ggactctcag tggccggcgc tcaaggccct gcccggcatt
     6961 cgcggtgaat tgggtgcaga ggcgcttgtt ttggtggatg gcggtatccg gcgaggtacc
     7021 gatgtgttca aggccctggc gctgggagcc gacgccgtgc tgatcggtcg cccttatatt
     7081 cacgccttgg ccacggccgg ggctttaggt gtggcgcatc tagtcaggct gctgcgcgag
     7141 gaactggaga tggccatggc attgagtggt tgccgcacct tgagcgagat aaatgccgac
     7201 ttgctgtgtc gcagcaatga ctggccctgc ccacctaaat aagccacgtg atatccaggg
     7261 cggggtttga gacttcgcga tcctcagtac aaatcccgcc gatagcgacc actctggctc
     7321 agttgttcca gctgctcatc gcccaccagt tgacgcaggc aggcgtcgac gtcgtggccc
     7381 atgccttcga ggctgccgca taggtagatg gcggcgccat cggccagcca ggcgtgcagg
     7441 gtgttggcgg cgtcgcgcag ggcgtcctgc acatagcgct gcgggtggcc gtcgcgcgaa
     7501 taggtgaggt cgagccgtgc gaggtggccg ctgtgctgcc aggcgcgcag ctcgtcgatc
     7561 agcaaggcgt cgtgggcggc gttacgttcg ccaaacacta gccaattgcg tgtgtgtgga
     7621 acggccgcgc gttcctgcag gtgcgcgcgt aggccagcga ggccgctgcc gctgccgatc
     7681 aggataagtg gcgtggcagc gtccgggcca tggaagccgg ggttgcggcg gatacgtagg
     7741 tcgatctggc tgccaacttc ggcatgttgg cagagccagc ctgagcccag gccggtgctg
     7801 ccgtcggcct gatattgcag gcgtaccagc agatcaagct ggccgctgcc gtggctggag
     7861 gcaatcgaat attcgcggtg gggcaggggc gttagttcgg ctagccactg ttctatggcg
     7921 tttttctcag ggcgggttgt gggcagatgg cgtgtgctca gggcatcgct tagcaactgg
     7981 tcgttagcca agcgggtgtg cggttcaagg ccgagttgct gcaggcaatc gataacccgc
     8041 tgcggggcat gtcgtgggcc gacttcgaca atatcgccgg cccgccaatc gacgtcttcc
     8101 ttgctgctca ggcgcagttg gtagatcggt gcgccgatgc tgccggggtt gaggcagttg
     8161 cgttgcgcca gttgccaggg gcggtagtgt gcgggctgcc agtcgacaaa gtcgctgttg
     8221 ccgctgaggt ggccaagctg ctgttgccag tggcgcaggg tgccggcatc gaggttgtcg
     8281 acgcacagca ggtcgaacag cggctgcgcc ttgagcgctt gcagacgctg ttgcaggcgt
     8341 tggccgaagg cgcagaactg cgggtagctg ctgtcaccca gagccagaat ggcgaagtgc
     8401 agttgcggca ggactggttg gctaagcagc gcgcgttcga agcgcgcggc gttgtccggc
     8461 gctt
//
LOCUS       DASRUM010000204         7169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_623023, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000204 DASRUM010000000
VERSION     DASRUM010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623023"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VFF22_13490"
     CDS             <1..320
                     /locus_tag="VFF22_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494935.1"
                     /note="penicillin-insensitive
                     transglycosylase/transpeptidase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HZX17859.1"
                     /translation="RSARLPAVDPQCPPQHVPVAAPLSIVGVREGDRLRRPAGSTEPL
                     RLRLSALGGSGRRWWFVDGQPIAETSADALFNHTFERNGQYQLSVLDESGQTALLLFR
                     VLD"
     gene            534..1283
                     /locus_tag="VFF22_13495"
     CDS             534..1283
                     /locus_tag="VFF22_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX17860.1"
                     /translation="MVCWALRLLCLSLLGSPLAMAAELQLYTEENPPLNFSRDGRADG
                     LASAVVRELLRRTGDSASIRVVPWARGYRQVQGKANVGLFVAVRTPEREPLFHWVGPL
                     VSTTTSFYARSGSGLRIANLEDAKRAAGIAVPRDWYSHQFLQKEGFTNLILQPKPHEV
                     LRMTLYGRVPVMVYEDELLPSLVTELGASMDDLKRLYSFMQSSSYIVFSKRTDVQLVQ
                     RWQQALTQMADDGSLARIQQQWLPQPALASP"
     gene            complement(1372..3303)
                     /locus_tag="VFF22_13500"
     CDS             complement(1372..3303)
                     /locus_tag="VFF22_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX17861.1"
                     /translation="MSVRKKLLSGFGLVLAITLGIAWISYGTLESVLGRFDTLLKVNE
                     IDSKLSLARQEEKNFIIRGDNQYLEQAQSLVGEIETLASDSLQLLTRPETITLMEGIR
                     QDVADYRQQLQALSQASASNQSAQRAMEEAARQALQEFDELQARLGKAAERQIRASGD
                     EDSIRTLGYANQASTLAKELLNARRFEKNFVISSQTQDAQVLLQQFDSLNLAGQQLRN
                     EINDPTARADLDSALAQLAIYQRNFDSLRQAVASRTTSEVAMNERARQVASASAESLE
                     LQLKVLQAEALQAEAILVGAAVIAFILGLFCALLITQVIVTPLQRVVQLARKVAQGDL
                     SENIESKRQDELGQLMQAMQTMTLSLRELLTRLTSGIEQLATAAEEMSAVTEQTSAGV
                     TQQKMETEQVATAMHEMTATVQDVARNAESAAVSARNADEQAQQGNHTVQQAIERIEN
                     LARSIEHSSQSIERLKHDSTNIGAVLDVIKGIAEQTNLLALNAAIEAARAGEMGRGFA
                     VVADEVRALAKRTQESTREIEGLIANLQGGAQSAVEMMSKSCTDASTTVDAAKQAGTA
                     LKAINSAVSSIQEMNQQIATAAEQQSSVAEEISRSVSNIRDVAEQSAAATEQTSAAST
                     DLARLGGELQTLVNRFKLA"
     gene            3625..4653
                     /locus_tag="VFF22_13505"
     CDS             3625..4653
                     /locus_tag="VFF22_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZX17862.1"
                     /translation="MTRNFYVNFFCLSGLLFSALLIANEWAGPKQSPPARSGMQVSFI
                     ASDFRNGGVVGVYRSFERAAKLLGWRVHAVDGRGDPAEIKRLTAEVLERKPDGVVLGG
                     FEPDYLGELHEAFQARKIALVGWHAGDRPGPTDKLFSNITSDPLQVADLAAEYAMRDG
                     QIGVVLFTDSQFAIATAKTQRMVQRLEACPQCKVLSVEDLPIASAGKGMDERVRSLQR
                     RFGAEWSHTLAINDVYFDHINVPLNALGRRDIRNISAGDGSAKALGRIHSGLSQQVAT
                     VAEPLGMQGWQLVDELNRAFAGQPPSLFVTPPLLVTSQVLQESDDLSIELDFSHEDAY
                     LRLWRQVD"
     gene            4784..6715
                     /locus_tag="VFF22_13510"
     CDS             4784..6715
                     /locus_tag="VFF22_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX17863.1"
                     /translation="MGLWKRSIQWQLILSMGAALLASILIVVGVYSAVVNRLTEHFLV
                     EEALPARVLAIRNDLERVLTAPITANAGIASNTFIQDWLAAGEEDGQRDAVARYLEGV
                     RAQQNAMTTSIAVLDSGNYFTGEGLARVISRDQPENQWFYKLVDSDRDQVLEIDIDKG
                     TRLPTLFINQRIKQGGKTLGVSGLGFSLKAMSEMIRDFRFGERGQVFLIDAQGDIKVH
                     PQAELSGSGRLAELFGKAPADQLLSSGGKAVRFEREGETYLAVAQKLSSLDWLLVSEV
                     PEAEIYAEARQALLMTSAIGVGVALLFLGLVVLLARGLVRPIRQVTVALVEIGGGGGD
                     LTRRLDESRADELGDLARGFNRFIGGQRSLISDVLATSERLRASVSQVAQVVDNTAGR
                     SSQQQEMTDMVATAVHEMGLTVQEIARNASSAAQSSQSARDEAQGARNEVRQSIGHIE
                     SMSTDIGNAAAAVSELAEQVASIDQVLAVIRGISEQTNLLALNAAIEAARAGEMGRGF
                     AVVADEVRTLASRTQSSTDEIQQMIQRLKQGAETAVSSMHAGQRATGTGVEASQRTGQ
                     SLSAITEQIERISDMNTQVATATEEQSSVTEEINRNVQGIADLAHATAGEVQVCKEDC
                     QTLRGLADDLAKQMSSFRL"
     gene            complement(6749..>7169)
                     /locus_tag="VFF22_13515"
     CDS             complement(6749..>7169)
                     /locus_tag="VFF22_13515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZX17864.1"
                     /translation="AQRQSQPLSLVLLDIDFFKRINDEYGHACGDSCLSDFAERMRQN
                     FRRDSDALLRLGGEEFGILMPNTPLEQARLLAEQFRLELEREGFNVPGATLPLTASLG
                     VGCYDMLRDGSAEAFFKRVDDALYQAKAAGRNRLELA"
BASE COUNT         1270 a         2024 c         2348 g         1527 t
ORIGIN      
        1 gacgcagtgc acgcctgccg gcggttgatc cgcagtgccc gccgcagcac gtaccggtcg
       61 ccgcgccgct gtcgattgtc ggtgtgcgcg aaggcgaccg tttacgccgt ccggccggca
      121 gtaccgaacc actgcgcttg cgcctctctg cactgggcgg cagtggccga cgttggtggt
      181 ttgtcgatgg ccagccaatt gcagaaacca gcgccgatgc gttgttcaat cataccttcg
      241 agcgcaacgg gcaatatcag ctcagcgtgc tggacgagag cgggcagacc gcgctgctgc
      301 tgtttcgcgt gcttgattga gtgtactccc gcatagggca tacagttggc catgggcaat
      361 gccgttcggg cggatttgtt ggttcgacta cactggggcg gactgaagta tcctgtgtca
      421 gtggcgctac gggctttttc tatcctgtag gcactgcccc ggtttggcgg ctcaggtgtt
      481 gggggagcgc gcaaaaggtc ctacgcatag gacgcatagt aagtggggta attatggttt
      541 gctgggccct gcgcttgttg tgcctatcgc tgcttggtag ccccctggcc atggcggctg
      601 agttgcagct gtacaccgag gaaaatccac cgctcaactt tagccgcgac ggccgggccg
      661 atgggctcgc cagtgcggtg gtgcgcgagt tgttacggcg taccggcgac agcgcgagca
      721 ttcgcgtggt gccctgggcg cggggttacc ggcaggtaca gggtaaggcc aatgtcggcc
      781 tgtttgtggc tgtacgcacc cccgagcgcg aaccgttatt tcactgggta ggcccgctgg
      841 tctcgaccac cacaagtttc tatgcccgca gtggcagcgg cctgcgcatt gccaatctgg
      901 aggatgccaa acgggctgcg ggcatcgccg tgccgcggga ctggtattcg caccagtttc
      961 tgcagaagga ggggttcacc aacctgattc tgcaacccaa gcctcatgag gtgctgcgca
     1021 tgaccttgta tggtcgggtg cctgtgatgg tttatgaaga tgagctgctg ccgagcctgg
     1081 tgaccgagct gggggcttcc atggacgacc tcaaacgcct ctacagcttt atgcagagca
     1141 gcagctatat cgtcttttcc aagcgcactg acgtgcagct ggtacagcgc tggcagcagg
     1201 ccctgacgca gatggctgac gatggcagtc tggcccgcat tcagcagcag tggctgccgc
     1261 aaccggctct cgcctcgcca taaagacgcg tactggatga gggcggtcag accgcgcgtc
     1321 tgtcctttca tcttgcgcac tgcggcagtc gtgccgcagt gcgcaacgtg ctcaggccag
     1381 cttgaagcga ttcaccaggg tctgcagctc gccacccagt cgcgccaggt cggtgctggc
     1441 ggctgaggtt tgctcggtgg cggcggcgga ctgttcggct acatcgcgaa tattggaaac
     1501 gctacggctg atctcttcag ccaccgagct ttgctgctca gctgccgtgg caatctgctg
     1561 attcatctcc tgaatgctcg acaccgcgct gttaatagcc ttcaaagctg ttccagcctg
     1621 tttggctgcg tccactgtgg tgcttgcatc ggtgcagctt ttactcatca tttccactgc
     1681 gctctgggca ccaccctgca ggttggcgat caggccttcg atctctctgg tagattcctg
     1741 ggtgcgtttg gccagggcgc gtacctcgtc ggctaccacg gcgaagccgc ggcccatctc
     1801 accggcccgg gcggcttcga tagcggcatt gagggccagc aggttggttt gttcggcgat
     1861 gcctttgatc acgtccagca ccgcgccgat attggtgctg tcgtgtttga ggcgctcgat
     1921 ggactgggat gagtgctcga tggaacgggc caggttttcg atgcgctcaa tggcctgctg
     1981 aaccgtatgg ttgccctgct gcgcttgctc atctgcatta cgcgcggaca cggctgcgga
     2041 ttcggcattg cgtgccacat cctgcacggt ggcggtcatt tcgtgcatgg cggtggcgac
     2101 ctgctcagtt tccatcttct gttgggtcac cccggcactg gtttgctccg tgacggccga
     2161 catttcttcg gcggcggtgg ccagctgctc gatgccactg gtcaggcggg tcagcagttc
     2221 gcgcaggctc agggtcatgg tctgcatggc ttgcatcaac tggcccagtt catcctggcg
     2281 cttgctttca atgttttccg agaggtcgcc ctgcgccacc ttgcgtgcca gctgcaccac
     2341 ccgctgcagg ggggtgacga tcacttgggt gatcagcagg gcgcagaaca atccaaggat
     2401 aaaggcgata acggcagcac caaccagtat ggcttcagcc tggagcgctt cggcctgcag
     2461 caccttgagt tgcagctcaa gggactcggc ggacgcgctg gccacctgcc gcgcccgctc
     2521 gttcatggct acttcgctgg tcgtacggct agccacggcc tggcgcaggc tatcgaaatt
     2581 gcgctgataa atggccagtt gggccagggc ggagtcgagg tcggcgcggg cggtggggtc
     2641 gttgatttcg ttgcgcagtt gctggcccgc caggttgagg ctgtcgaact gctgcagcag
     2701 cacctgagca tcctgggtct ggctgctgat aacaaagttt ttctcaaagc gcctggcatt
     2761 gagcagttct ttggcaagtg tgctggcctg gttggcgtag ccaagggtgc gaatgctgtc
     2821 ttcatcaccg ctggcgcgga tctgccgctc ggcggctttc cccagcctgg cctgaagctc
     2881 gtcgaactcc tgcagcgctt gccgcgctgc ttcttccatg gcccgttgag cgctctggtt
     2941 gctggcgctg gcctggctaa gcgcctgcag ctgctggcgg taatcggcta catcctgacg
     3001 aataccttcc atcagggtga tggtttccgg gcgggtcaat aactgcaggc tgtcgctggc
     3061 cagggtttcg atctctccga ctaggctttg ggcctgctcg aggtactggt tgtcgccacg
     3121 gataataaag ttcttttcct cctggcgggc cagcgacagc ttgctgtcga tttcgttgac
     3181 cttgagcaag gtatcgaagc gccccaggac gctttccaga gtgccgtagc tgatccaggc
     3241 gatgcccagg gtgatggcca gcaccaggcc aaaaccgctg agcaacttct tccgtacgct
     3301 catatctagg atgaaattgc tgactgatcc ggccataaga gcttctctct tcggttattt
     3361 agaggcagaa aagaatctta gaggcttgat ggcatatggc tatgggggat tctttacatc
     3421 gcgtttcagg aagagcgggc cgtctgataa cgacgaccta tggcagctgg tcggcgcagc
     3481 cgtgaccaga tgctagaaat cgatatcgac aagggggtgt gtcggactag ggttacggcg
     3541 caatgctgtc atcgccatga gtgactacgc tattaggtga aggttggcac agtcgtctgc
     3601 cggccagtca cgcagggata ggcgatgacg agaaatttct acgtaaactt cttctgtctg
     3661 tccgggctgt tgttcagcgc gctgctcatt gccaatgagt gggccgggcc gaagcagtcg
     3721 ccgcccgcac gcagtggcat gcaggtgagt tttatcgcat cggattttcg caacggtggg
     3781 gtggtcgggg tgtaccgcag ctttgagcgt gcagccaagt tgctgggctg gcgggtgcat
     3841 gccgtggacg gtcgtggcga tccggcggag atcaaacgtt tgacggctga agtgcttgag
     3901 cgcaagccgg atggcgtggt gttgggcggc ttcgagccgg attacctggg cgaactgcac
     3961 gaggcttttc aagctcgcaa gattgccttg gtcggctggc acgccgggga ccggccggga
     4021 ccgacagaca agctgttcag taatatcacc agcgacccct tacaggtggc cgacttggct
     4081 gccgagtacg ccatgcgtga cgggcagatt ggcgtggtgc tgtttaccga cagccagttc
     4141 gctatcgcta ccgccaagac ccagcgcatg gtgcagcgtt tggaggcgtg cccgcagtgc
     4201 aaagtgctga gtgtcgaaga cctgccgatt gccagtgccg gcaagggtat ggatgagcgt
     4261 gtgcgttcgt tgcagcgccg ctttggcgcc gagtggagcc ataccctggc gatcaacgat
     4321 gtgtacttcg accatatcaa tgtgccgctc aatgctcttg gtcgtagaga cattcgcaat
     4381 atttctgctg gcgatggctc ggccaaggcg ctgggtcgca tccattcggg gttgtcccag
     4441 caggtcgcca cggtcgccga gccgctgggc atgcagggtt ggcagctggt cgatgagctg
     4501 aaccgagcct ttgccggcca gccgccaagt ctgtttgtaa ccccgccgct cttggtcact
     4561 tcgcaggtgc tgcaggagtc agatgatctc agcatcgagc tggacttcag ccatgaagat
     4621 gcctatctgc ggctgtggcg gcaggtggac tgaggcattg ccgatagctg gttaaagttg
     4681 gcagtcggca cagccgatag tgttatatgt cgctgaacaa ttgagtttgt atacaaaatt
     4741 gtattcaatt gttgtcttca aataataact agaaggtaac cccatgggct tgtggaaacg
     4801 cagcattcag tggcagctga ttctcagcat gggcgcggcc ctgcttgcca gtattctgat
     4861 cgtggtcggc gtttattcgg cggtggtcaa tcgcctgacc gagcactttc tggtcgaaga
     4921 ggccttgccc gcgcgggtgc tggcgattcg caatgatctg gagcgggtgc tgaccgcgcc
     4981 gatcaccgcc aacgcaggga ttgccagcaa tacctttatt caggactggt tggcggcggg
     5041 cgaggaggac ggtcagcgcg atgccgtggc gcgttatctg gagggcgttc gtgcgcagca
     5101 gaacgccatg accacctcga ttgccgtgct ggacagcggc aactacttca ccggtgaggg
     5161 cttggcccgg gtgatcagcc gtgatcagcc ggaaaaccag tggttctaca agctggtcga
     5221 cagcgaccgt gaccaggtgt tggaaatcga tatcgacaag ggcacgcgcc tgccgaccct
     5281 gtttatcaat cagcggatca agcagggcgg caaaaccctg ggcgtttccg gtctggggtt
     5341 cagcctgaaa gccatgtcgg aaatgattcg cgattttcgc tttggcgagc gcggtcaggt
     5401 gtttctgatc gatgcccagg gcgatatcaa ggtgcatccg caggccgagc tgagcggcag
     5461 tggccggctt gccgagctgt ttggtaaagc gccggccgat caactgctga gcagcggtgg
     5521 caaggcggta cgcttcgagc gtgaggggga aacctacctg gcggtggcgc agaagctctc
     5581 cagcctggac tggctgctgg tcagcgaagt gccggaagcg gaaatctatg ccgaagcccg
     5641 gcaggcgctg ctgatgacca gcgccatcgg ggttggcgtg gccttgctgt tcctcggtct
     5701 ggtggtgctg ctggcgcgtg gcctggttcg accaatccgt caggtaacgg tggcgctggt
     5761 ggaaatcggt ggcggcggtg gcgatctgac caggcggctg gatgagtccc gcgcggatga
     5821 gttgggcgat ctggcacgcg gctttaaccg ctttatcggc ggccagcgca gcctgatcag
     5881 cgatgtgttg gcgaccagtg agcgcttgcg cgcctcggtc agtcaggtgg cgcaggtggt
     5941 cgataacacc gctgggcgtt ccagccagca gcaggaaatg accgatatgg tcgccacggc
     6001 ggtgcatgag atgggcctga ccgtgcagga aatcgcgcgc aatgccagca gtgccgcaca
     6061 gtcatcgcaa agcgcacggg acgaggcgca aggcgcgcgc aatgaggtgc gccaatccat
     6121 cgggcatatc gagagcatgt ccactgatat tggcaatgcc gccgccgcag tcagtgagct
     6181 ggccgagcag gtggcatcga ttgaccaggt gctggcggtg attcgcggga tttccgagca
     6241 gaccaacctg ctcgcgctca acgccgccat cgaagcggcg cgggccgggg aaatgggccg
     6301 tggctttgcc gtggtggccg acgaggtacg cactctggcc agtcgcacgc aaagctctac
     6361 cgatgaaatc cagcagatga ttcagcgcct caaacagggc gcggaaacgg cggtaagttc
     6421 catgcatgcc ggccaacgtg ccacgggtac tggtgtcgag gccagtcagc gtacggggca
     6481 gtcgcttagc gcgattaccg agcagattga acgcatcagc gacatgaaca cccaggtggc
     6541 caccgccacc gaggagcagt cgtcggtgac cgaagagatc aaccgcaacg tgcagggcat
     6601 tgccgatctg gcgcatgcca ccgccggtga ggtgcaggtg tgtaaagagg actgtcagac
     6661 cctgcgcggc ctggccgatg atctggccaa gcagatgagc agtttccgtt tgtaagcgta
     6721 tgccggcggc acgcctgtgc cgccggcatc aggccagctc tagacgattg cgcccggcag
     6781 ctttggcttg atagagcgca tcgtcgacgc gcttgaagaa cgcttcggca ctgccgtcgc
     6841 gcagcatgtc ataacacccg acacccaggc tcgcggtcag cggcagagtc gctcccggta
     6901 cgttgaagcc ttcgcgctcc agctccagac gaaactgctc ggccagcagg cgtgcctgtt
     6961 ccagcggggt gttgggcatc aggatgccga actcttcacc gcccaggcgt agcagcgcgt
     7021 cgctgtcacg gcggaagttc tggcgcatgc gttcggcaaa gtcgctcagg cagctgtcac
     7081 cgcaggcatg cccgtactcg tcgttgatcc gcttgaagaa gtcgatatcc agcagtacca
     7141 gcgacaacgg ctggctctgc cgctgggcg
//
LOCUS       DASRUM010000205        13465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_103989, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000205 DASRUM010000000
VERSION     DASRUM010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 13465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13465
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103989"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="VFF22_13520"
     CDS             complement(<1..121)
                     /locus_tag="VFF22_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17865.1"
                     /translation="MTVIKQDDLIQSVADALQFISYYHPVDFIQAMHEAYLREE"
     gene            423..1550
                     /locus_tag="VFF22_13525"
     CDS             423..1550
                     /locus_tag="VFF22_13525"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZX17866.1"
                     /translation="MSLSSQRVTQRSLQSLLLKRFGMAVATYALTGLLCWIGVFAGLF
                     NASPLAALTITALAALSQLVFLGLFLSRINLRLADPSLTEPQVLVALAWLTVLLSLFS
                     EGRGSMLVFYLLILLFGVFQLPPRVFARCALFAFFGFAGLNLYEAYTLQLSEPRVALM
                     QACVLAVVLVWLCLFASYAQAMRQRMRQRRFALQAHQDTLRGMMRQLEDLAATDELTG
                     LCNRRHFLRLASRELEGLRNGRQHGLALLDLDHFKRINDIHGHAAGDRVLQTFAAVAR
                     ACLRDGDVVARYGGEEFVLLLPNTEADQFTACCERLREAFSRAEPLGVKVASLSVSIG
                     MTLLTVHDDLDEALQRADQALYQAKRDGRNRCAATWEDVDA"
     gene            1543..2484
                     /locus_tag="VFF22_13530"
     CDS             1543..2484
                     /locus_tag="VFF22_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur-binding ferredoxin reductase"
                     /protein_id="HZX17867.1"
                     /translation="MPELRAAGRCLEVAPASNLLDNLLAAGIAVPYSCRAGSCHACLV
                     RCVGGELLDGKPEALDAARREQGWRLACQCRVVEDVQIEVFDPLRDAAPAVIHSCDWL
                     SSDVLRLRLTPQRPLRYSAGQHLQLWTEGGIARPYSLASVPGDDPWLEFHIDCRQGGA
                     FATAARAFQPGGNLRLGELRGAALHYDPDWQTRPLWLMAAGTGLAPLYGVLREALRQE
                     HQGTIRVIHLAHDAAAHYLAEPLQALAASHPQLQVELLVAAELPAALAELRLVSRQTI
                     ALLCGHPDSVETFARRLYLAGLPRSQVFADVFLPHAR"
     gene            2531..3304
                     /locus_tag="VFF22_13535"
     CDS             2531..3304
                     /locus_tag="VFF22_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HZX17868.1"
                     /translation="MCEHLLVEREQGLLTLRMHRPDKKNALTRAMYSGMAEVLVQADQ
                     DKSVRAVLITGGESCFTSGNDVADFIKAPPTGLNSEVFQFMQALFEFSKPVVAAVNGP
                     AVGIGTTMLLHCDLVYVSRDATLKMPFVNLGLCPEYGSSLILPRLLGHARAAELLLLG
                     QSFTGEQAATWGIANQALDSGAATLAKAREMALRFQQLAPAAVADSKRLMLAPDREQL
                     RRVIEEEGVLFGQRLRSPEAIEALTAFMQRRTPDFSKFA"
     gene            complement(3365..4816)
                     /gene="pyk"
                     /locus_tag="VFF22_13540"
     CDS             complement(3365..4816)
                     /gene="pyk"
                     /locus_tag="VFF22_13540"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZX17869.1"
                     /translation="MTIRRTKIVATLGPSSNSPEVLEQLIIAGLDVARLNFSHGTPDE
                     HKARAKLVRELAAKHGRHVALLGDLQGPKIRIAKFADKRIELKLGDRFTFSTSHPLTA
                     GTQEIVGIDYPDLVKDCGVGDELLLDDGRVVMRVVSATSDALHCEVIIGGPLSDHKGI
                     NRRGGGLTAPALTEKDKADIKLAAEMELDYLAVSFPRDADDMHYARKLRDEAGGTAWL
                     VAKIERAEAVADDETLDGLIRASDAVMVARGDLGVEIGDAELCGIQKKIILHARRHNK
                     AVITATQMMESMIQNPMPTRAEVSDVANAVLDYTDAVMLSAESAAGAYPLEAVQAMAR
                     ICLGAEKHPTSKTSSHRMGTEFERCDESIALAAMYTANHFPGVKAIIALTESGYTPLI
                     MSRIRSSVPIFAFSPHRETQARAAMFRGVYTVPFDPAALQPAEVSQAAVDELLKRGVV
                     QAGDWVILTKGDSYHTIGGTNTMKLLHVGEALV"
     gene            complement(4897..5694)
                     /locus_tag="VFF22_13545"
     CDS             complement(4897..5694)
                     /locus_tag="VFF22_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZX17870.1"
                     /translation="MSSSSRLLVLLCTALLSACSPTTPLFIAQITTNEVVEYKTLKNN
                     RMLAAIEDEGDLLTYSAQAIRDAKSEQAEQLYLTGYRDEKLSDEVRAISLYQIGLLYM
                     NRFNDQRDDNKALNYFYQVLNQFPASRAAERAEARIVIIRKRADEPVQKTSRELLVHW
                     KPNQQLDLYKPSLDPDMTLLSRRAVLKNRVSEAEELYLLALSDPGIPSDIKEKALYQL
                     GLMYLAPDNQQASRDKAITYLRRLLVQYPSSELNNKAARHLDQALNR"
     gene            5844..6218
                     /locus_tag="VFF22_13550"
     CDS             5844..6218
                     /locus_tag="VFF22_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZX17871.1"
                     /translation="MRILVLVLLMLSVVGCSRHSLDRHLNNAYEAYDRGECEEAILSL
                     SRAERSSRSRNYIQPEISLLRGQCLERQALFVDAMQTYQFIVTRYPASEYAYRARARM
                     DTLRQLGHDGVDVAVKVNPVKP"
     gene            6275..6697
                     /locus_tag="VFF22_13555"
     CDS             6275..6697
                     /locus_tag="VFF22_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HZX17872.1"
                     /translation="MRVLLCLLLLPGLAAAEIYRWTDANGQVHFGQRPAAAGAERVEV
                     KPQVVERDQLTREREERTNRFYDARREEQVQASAVAAENQAKRAEECRELRNKLAAIP
                     EGRSYYRKEANGERSYYSDEQVDTARQQLQSRVAERCS"
     gene            6758..7120
                     /locus_tag="VFF22_13560"
     CDS             6758..7120
                     /locus_tag="VFF22_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZX17873.1"
                     /translation="MPKHIQRSIERHQLPYYLKVFNRITDKPMGYIGNVSLDGLMLIS
                     QLPLLVGARFDMRLKIPGHDAPHYIDFSATCQWSREDVTPGCYDSGFALVAPPGDYVE
                     MVDALRHYFSFRPMAASA"
     gene            complement(7185..8687)
                     /gene="mqo"
                     /locus_tag="VFF22_13565"
     CDS             complement(7185..8687)
                     /gene="mqo"
                     /locus_tag="VFF22_13565"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HZX17874.1"
                     /translation="MAQNDYETMDVVLVGAGIMSATLAVLLKELDPAIKLEVLEMMAS
                     GAIESSNPWNNAGTGHAGLCELNYTPEAADGSIDIKKSVTINTQFEESKQFWAYLVEK
                     GRFGSPKSFINPVPHLSFVRGQKGIDYLKKRFDALTGHHAFAEMEYTEDRATMAEWMP
                     LMLPGRDPNEPIAATRVMAGTDVNFGTLTTHLLDHLGQQADAKVTCSQKVVGLKRNAD
                     GWRVSVKDQQNGSSREIQAKFVFLGAGGAALPLLQMSGIPEGKGFGGFPVSGQWLRCD
                     NPDVVKQHQAKVYSQAAVGSPPMSVPHLDTRVVDGKTSLLFGPYAGFSTKFLRHGSFL
                     DLPLSVRPNNIGPMLAVARDNFDLTRYLVKEVLQSEEQRLETLRGFYPEAKAEDWRLE
                     MAGQRVQIIKKDAKNGGILQFGTELVAAEDGSIAALLGASPGASVTVSIMLDLIQRCF
                     GAQVKSKQWSSKLNEIFPAMGAVLASDAERYREVQSRSDALLQLEQTVSA"
     gene            complement(9146..9379)
                     /locus_tag="VFF22_13570"
     CDS             complement(9146..9379)
                     /locus_tag="VFF22_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17875.1"
                     /translation="MFSLRNLLAPRRPMRTFALVDAQGICRALRQSAQAPQGSGWIEV
                     QHSCMSWLNRPLPAGVRTVQVTPCLRPHQTLAA"
     gene            9552..10136
                     /locus_tag="VFF22_13575"
     CDS             9552..10136
                     /locus_tag="VFF22_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007973847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajG family lipoprotein"
                     /protein_id="HZX17876.1"
                     /translation="MLHRLLFGLVAVASLTLVGCAHSPQQLSPQPKLNSPLTAVGQGQ
                     PVVVRVADGRPSPVLGTRGGLYPETSAISVTGQDILPKLQAQAEAAVRLLGFTPSANA
                     YNAPQLTLTLAELKYQSPKEGMYVTEADMTATFRLDVQNSSRRYSGRYGASLNQRFGM
                     APNQATNTKLISDVLSDALTRAFKDPTISQMLAQ"
     gene            complement(10201..10407)
                     /locus_tag="VFF22_13580"
     CDS             complement(10201..10407)
                     /locus_tag="VFF22_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005891226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17877.1"
                     /translation="MLHAQPQDRDYLIDSLDDIDAVIDGLSFNVQDDHWLVYCAMGGH
                     EHYALPDIDTRTGMSLPELYSEAA"
     gene            10677..10964
                     /locus_tag="VFF22_13585"
     CDS             10677..10964
                     /locus_tag="VFF22_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003146038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17878.1"
                     /translation="MSLQALWLLIEGQSGQWLNGLALFFALAGAWLLLATRMREQRAR
                     ARLLAGNQLNELADEAYAFDEPTLRLNRFFYCFGHACLAAALLLSWLSTQL"
     gene            complement(11029..12195)
                     /locus_tag="VFF22_13590"
     CDS             complement(11029..12195)
                     /locus_tag="VFF22_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015636954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HZX17879.1"
                     /translation="MMGEFDAIRPYADAEVPAVLARLLADDAFLGILTQFRFPRLAAP
                     LGWLLKPLIAYRLRLEFRDVSSVAQLQTRIESYVDRTIEQATDGVSYSGIEHLQAGNA
                     YLFLANHRDIVMDPAFVNYAVYHAGLPTPRIAIGDNLLQKPFVSDLMRLNKSFIVHRS
                     ISGRREKLAAYQLLSAYINHSIRNDGESIWIAQAEGRAKDGDDRTDSAILKMFHMSRK
                     GEPFAEVINALRLTPVSISYEYDPCDQAKARELFIRASSGSYTKAAGEDDQSIALGIT
                     GYKGRVHIHFGAPVSQGLEDSKLLAAEMDRQILSGYRLFPVHYLAYAMWADRDPELAV
                     PSAAELFPAAELSAAKSEWQRRLANCPSEQQPYLILQYANPVRNQYRIKAGLPL"
     gene            12324..12506
                     /locus_tag="VFF22_13595"
     CDS             12324..12506
                     /locus_tag="VFF22_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008719780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HZX17880.1"
                     /translation="MLESQDYQCPYCGEQVEALLDLSAGDQQYIEDCPVCCRPIVFSL
                     QTDGEDWSLDVRGEND"
     gene            12506..12766
                     /locus_tag="VFF22_13600"
     CDS             12506..12766
                     /locus_tag="VFF22_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HZX17881.1"
                     /translation="MQRIYEPQDLMEGELLLAMLASEGIEAYLTGRHLAGAVGELPAC
                     GLLGVMVEDEQAHSAQQLIAAYNAALPLPGDEPDSFQGTLLC"
     gene            12832..13449
                     /locus_tag="VFF22_13605"
     CDS             12832..13449
                     /locus_tag="VFF22_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010565716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HZX17882.1"
                     /translation="MCGRYALFRWTPAFAAIPGFPADQLPHWSISPNSQVLLLRNVEG
                     ERQLTRARWGLTPPWLTDLTKTPAQARAETLAEQPMFREAFRLRRGLLPANGFYEWRG
                     TTRKRPYWLTGETTPLYFAALWEAYPVEGCVYLSAAVVTQAAASQRRPLILDEAGQAA
                     WLAADTPAADLQALLASPPPALRERVLANLINDPKLDAPECLTPA"
BASE COUNT         2385 a         3921 c         4322 g         2837 t
ORIGIN      
        1 actcttcgcg caggtaggcc tcatgcatgg cctggataaa atccacgggg tggtaatagg
       61 agatgaactg cagggcgtcg gcgacgctct ggatcaggtc gtcttgcttg atcacggtca
      121 tgcttggcgc tcctttttgt aaaggtagga gtagggcagg aacttcgaac agcgctgggt
      181 cacggcccgg cgcagtgcta tcaaggtcaa aaggcaaggt caaaggacaa ggccaaagca
      241 caagataaaa aggcgcggca gtataccgcg cacgacgtca ggcggacacc tgtacggccc
      301 cagaccatag tcgctgatcg gccatttgag ccgactaatg acgcggcagc ggcctaggca
      361 ttgtctgagc acaggggtac agtggcggct agatgccagc atgggacgga gcccataaac
      421 ccatgagcct aagtagccag cgggtaaccc agcgatctct gcaaagcctg ctgctgaagc
      481 gtttcggcat ggcagtggca acctatgcgc tgaccgggct gttgtgctgg attggcgtat
      541 ttgccgggct gtttaatgca tcgcccctag ccgctcttac tatcaccgcc ctggccgcac
      601 tgagccagct ggtgtttctc gggttgtttt tgtcccgcat caatctgcgt ttagcagacc
      661 ccagcctgac cgaaccccag gtgctggtgg cattggcctg gctgaccgtg ctgctgtcgc
      721 tgttcagcga agggcgcggc agcatgctgg tgttctacct gctgattctg ctattcggtg
      781 tgttccagct gccgccacgg gtgttcgctc gctgcgcgct gtttgccttt tttggctttg
      841 ccggcctgaa cctgtatgaa gcctataccc tgcagctgag cgaaccgcgt gtggcgctga
      901 tgcaggcgtg cgtgctggcg gtggtgctgg tctggctgtg cctgtttgcc agttacgccc
      961 aggccatgcg tcagcgtatg cgccaacggc gttttgccct gcaggcgcat caggacacgc
     1021 tgcgtggcat gatgcgtcag ctggaagacc tggctgccac cgatgagctg accggcctgt
     1081 gcaaccgtcg gcacttcctg cgcctggcca gccgtgagct ggaagggctg cgcaacgggc
     1141 gccagcatgg cctggcgttg ctcgatctgg atcatttcaa gcggatcaac gatatccatg
     1201 gccacgccgc cggggatcgg gttctgcaga cattcgccgc tgtcgcccgc gcctgcctgc
     1261 gcgacggcga cgtggtagcc cgctacggcg gtgaggaatt tgtgttgttg ttacccaata
     1321 ccgaagccga tcagttcacc gcctgctgcg agcgcctgcg tgaggccttt agccgcgccg
     1381 agccgctggg cgtgaaggtg gccagcctga gcgtgtccat cggcatgacg ctgctgaccg
     1441 tgcacgatga tctcgacgaa gccctgcaac gtgccgatca ggcgctgtac caggccaaac
     1501 gcgacggccg taaccgttgc gcggcgacct gggaggatgt ggatgcctga gttacgggcc
     1561 gctggtcgct gccttgaggt cgcccccgcc agtaacctgc tggacaacct gctggctgcc
     1621 ggcatcgccg tgccctacag ctgccgcgcc ggcagttgcc atgcctgcct ggtgcgctgc
     1681 gtgggtggcg aattgctcga tggcaaaccg gaggccctgg acgctgcacg gcgcgaacag
     1741 ggctggcgct tggcctgcca gtgccgggtg gtcgaggacg tgcagattga ggtgttcgac
     1801 ccgctgcgtg atgcggctcc agccgttatt cacagttgcg actggctcag cagcgatgtg
     1861 ctgcgtctgc gcctgacccc acagcggccg ctgcgttaca gcgccggcca gcacctgcaa
     1921 ctctggacag agggcggcat tgcccggcca tattccttgg ctagcgtgcc gggcgatgat
     1981 ccttggctgg agtttcatat cgattgccgc cagggcggtg cgtttgccac ggcggcgcgg
     2041 gcgtttcagc cgggcggtaa cctgcggttg ggcgaattgc gcggtgcggc gctgcactat
     2101 gacccggact ggcaaacccg cccgctgtgg ctgatggccg ccggcaccgg gctagcgccg
     2161 ctgtacgggg tgctgcgtga ggcactacgg caggagcatc aaggcacgat ccgggttatt
     2221 cacttggccc atgatgcggc cgcgcattac ctggccgagc ccttgcaagc gttggcggcc
     2281 agccatcctc agctgcaggt cgagctgctt gtcgcggccg agttgccggc tgctttggcc
     2341 gaactgcgcc ttgtttcacg gcaaaccatc gccttactct gcggccaccc cgacagcgtc
     2401 gaaacttttg cgcgccgctt gtatctggcg ggtttaccgc gcagccaggt tttcgctgac
     2461 gtctttttgc cccacgcgcg ctagccgttc aggttctcag ctcgcgggtg gcctgccgtg
     2521 aggattgatg atgtgcgagc acctgctggt tgagcgtgaa caagggctgt tgaccctgcg
     2581 catgcaccgc cctgacaaga agaatgccct gacccgcgcc atgtacagcg gcatggccga
     2641 agtgctggtg caggccgatc aggataaaag cgtgcgcgcc gtgctgatca ctggcggcga
     2701 gagctgcttt accagcggca acgatgtcgc cgactttatc aaggcgccgc ccactgggct
     2761 gaacagcgaa gtgttccagt ttatgcaggc actgttcgag ttcagtaaac cagtggtggc
     2821 cgcagtcaac ggcccggcgg tgggcatcgg tacgaccatg ctgctgcact gcgacctggt
     2881 gtatgtcagc cgcgatgcca cgctgaaaat gcctttcgtc aaccttggcc tgtgccccga
     2941 atacggttcc agcctgattc tgccgcgttt gctcggccac gcccgcgctg ccgaactatt
     3001 gctgcttggg cagagcttta ccggtgagca ggccgcaacc tggggcatcg ccaatcaggc
     3061 gctggatagc ggcgcggcaa ccctggccaa ggcccgcgaa atggccctgc gcttccagca
     3121 attggcaccg gctgcagtgg cggacagcaa acgcctgatg ctcgcaccgg atcgcgagca
     3181 actgcgccgg gttatcgagg aggagggcgt cttgttcggc caacgcctgc gctcgccgga
     3241 agccatcgag gcgctgactg cctttatgca gcggcgcacg ccggacttca gcaagttcgc
     3301 ctgatgccct gaggcaaaca aaaaggccgc tcgattgagc ggccttttgc attgcagcgc
     3361 ggtgttacac cagcgcttca ccgacatgca gcagcttcat ggtgttggtg ccgccgatag
     3421 tgtggtagct gtcgcccttg gtcaggatca cccaatcgcc ggcctgcacc acgccgcgct
     3481 tgagcagctc gtccaccgcg gcctggctga cttccgccgg ttgcaaggcg gctgggtcga
     3541 acggcacggt gtagacgccg cggaacatgg ccgcgcgggc ctgggtttcg cggtgcggag
     3601 agaaggcgaa gatcggcacc gaagaacgga tgcgcgacat gatcagcggc gtgtagccac
     3661 tttcggtgag ggcgatgatc gctttcacgc cggggaagtg gttggccgtg tacatggccg
     3721 ccagggcgat gctttcgtcg cagcgctcga actcggtgcc catacggtgg ctggaggtct
     3781 tgctggtcgg gtgcttttcc gcgcccaggc agatgcgcgc catagcctgc accgcttcca
     3841 gcgggtaggc gccggcggcg ctttccgccg agagcatcac ggcatcggtg tagtcgagca
     3901 cggcgttggc cacgtcggag acttccgcgc gggtcggcat cgggttctgg atcatcgact
     3961 ccatcatctg cgtcgccgtg atcaccgcct tgttgtggcg gcgggcgtgc agaatgattt
     4021 tcttctggat gccgcatagc tcggcgtcgc cgatttccac gcctagatca ccacgggcaa
     4081 ccatcaccgc atcactggcg cggatcaggc cgtcgagggt ttcgtcatcg gccacggctt
     4141 cggcgcgttc gatcttggcc accagccagg cggtgccgcc tgcttcatca cgcagcttgc
     4201 gcgcgtaatg catgtcgtcg gcatcgcgcg ggaaggacac ggccaggtaa tccagctcca
     4261 tctcggcagc cagtttgatg tcggctttgt ctttctcggt cagcgccggc gcagtcaggc
     4321 caccgccacg gcggttgatg cctttatggt cggacagcgg gccgccgatg atcacttcgc
     4381 agtgcagggc atcgctggtg gcactgacaa cccgcatcac cacgcggcca tcgtcgagca
     4441 acagctcgtc accgacgccg cagtccttga ccaggtccgg gtagtcgatg ccgacaatct
     4501 cttgcgtacc ggccgtcagc gggtggctgg tggagaaggt gaagcggtca ccgagcttga
     4561 gctcaatgcg cttgtcggcg aatttggcaa tacggatctt cgggccctgc aggtcgccga
     4621 gcagggcaac gtggcgacca tgcttggcgg ccagctcacg caccagcttg gcgcgggctt
     4681 tgtgctcatc gggcgtgccg tgggagaaat tcaggcgggc gacatccaag ccagcgatga
     4741 tcagctgttc aagcacttcg gggctgttgc tggatgggcc gagggtggcg acgattttgg
     4801 tgcggcgaat ggtcatgcac agactcctga gttgcatctg agcacgaggc tactatgccg
     4861 caacgctgta gtcattgttc ctctgcacta ccagacttat cggttgagtg cctggtccag
     4921 atggcgagcg gccttgttgt tcagctcgct gctggggtat tgcaccagca ggcggcgcag
     4981 gtaggtgatg gccttgtcgc ggctggcctg ttgattgtcc ggggccaggt acatcagacc
     5041 gagctggtag agggcctttt ccttgatgtc ggaggggatg ccggggtcgc tcagggccag
     5101 caggtacagc tcttcggcct cgctgacgcg gttcttcagt acggcgcggc gcgacagcag
     5161 ggtcatgtct gggtcgaggc tgggcttgta caggtcgagc tgttgattgg gtttccagtg
     5221 tacaagcagt tcgcgcgagg ttttttgcac gggctcatca gcacgtttac ggatgatcac
     5281 gatgcgtgct tctgcgcgtt cggcggcgcg gctagcgggg aactggttga gcacctgata
     5341 gaagtagttg agtgccttgt tatcgtcgcg ctggtcgttg aaacggttca tgtacagcag
     5401 gccgatctgg tacagcgaga tggcgcgcac ctcgtcgctg agcttctcgt cgcggtagcc
     5461 ggtgaggtac agctgctctg cctgttcgct cttggcgtcg cggatggctt gggcgctgta
     5521 ggtcagcaga tcgccttcat cctcgatggc ggcgagcatg cggttgttct tcagggtttt
     5581 gtattcgacc acttcgttgg tggttatctg tgcaataaac agcggtgtgg tgggtgagca
     5641 ggcgctcaac agagcggtac acagcaacac tagcaggcga gaggaagacg acatggcagc
     5701 tcctgggccc aatggccgtt gcgcgcaagt ctagtcaggc gggtggacac tggccaagtg
     5761 gctggccgca ttacccatca gaggatatgt tgctgcagaa ttttcctggg cagtcgatac
     5821 actgctcatt ggaggagagg cttatgcgaa ttcttgtgtt ggtactgctg atgctgagcg
     5881 tggtggggtg tagccgccat agtctggata ggcatttgaa caatgcctat gaggcttatg
     5941 atcgtggtga atgcgaggaa gcgatccttt ctctgtcaag ggcagagcgc agcagccgct
     6001 cgcgcaacta catccagccg gaaatttccc tgctgcgtgg ccagtgccta gagcgtcagg
     6061 cattgtttgt cgatgccatg cagacctatc agttcatcgt cactcgttac cctgccagtg
     6121 agtatgctta ccgcgcccgc gcgcgtatgg atactctgcg tcaactggga cacgacggcg
     6181 tcgatgtggc agtcaaggtc aatccggtca agccctgact gctggttgtt acggagcata
     6241 gaaggctaag ctgagtcttt acgggaggga atccatgcgc gttttgttgt gcctgctgtt
     6301 attgccgggg ttggcggctg cggaaatcta ccgctggacc gatgccaatg gccaagtgca
     6361 tttcggccag cgtccagcgg cagctggggc cgagagggtc gaggtcaagc cgcaggtggt
     6421 tgagcgtgac cagctgaccc gtgagcgcga agaacgcacc aatcgtttct atgacgcacg
     6481 ccgcgaggag caggtgcagg cttcggcggt ggctgcggaa aaccaggcta agcgtgccga
     6541 ggaatgccgc gaattgcgta acaaactggc cgcgattccc gaagggcgca gctattaccg
     6601 caaagaagcg aacggcgaac gcagttacta cagcgatgaa caagttgata ccgctcgtca
     6661 gcaactgcag agcagggtgg ctgaacgttg ttcttgaaac tggtttaagc gcagtagtgg
     6721 ggccatactc aatggccatc tatagcgatt tgccactatg cccaagcata tccagcgcag
     6781 catcgaacgc catcagttgc cctactacct gaaggtgttc aatcgcatca ccgacaagcc
     6841 catgggctat atcggtaacg tctccctgga cgggctgatg ctgatcagcc agctgcccct
     6901 gctggtgggc gcacgtttcg acatgcgcct gaaaatccct gggcacgacg cgccgcacta
     6961 catcgatttc tccgctacct gccaatggtc ccgcgaggat gtcaccccag gctgctatga
     7021 ctctggcttc gccctggtcg ccccgccggg cgactatgtc gagatggttg atgcgctgcg
     7081 tcattacttc agcttccgcc ctatggctgc gtcggcctga cttgaccgcg tttggccgga
     7141 cataaaaaac cgcccctaga ggcggttttt tattgcggcg cggtttaggc gctgacggtc
     7201 tgttccagct gcagcaacgc atcggagcgc gactgcactt cgcgatagcg ttcggcatcg
     7261 ctggcgagca ccgcccccat ggcggggaag atctcgttca gcttgctgct ccactgcttt
     7321 gatttgacct gggcgccgaa gcagcgctgg atcagatcaa gcatgatcga cacggtgacc
     7381 gatgcacctg gcgatgcacc gagcagggcg gcgatggagc cgtcttctgc cgccaccagt
     7441 tcggtaccga actgcaggat gccgccgttc ttcgcgtcct tcttgatgat ctgcacgcgt
     7501 tggccggcca tctccaggcg ccagtcttcg gctttggctt ccgggtagaa gccgcgcagg
     7561 gtttccaggc gctgctcttc ggactgcagc acttccttga ccaggtaacg cgtcaggtcg
     7621 aagttgtcgc gtgccactgc cagcatcggg ccgatgttgt tcgggcgtac cgacaacggc
     7681 agatcgagga aggaaccatg gcgcaggaac ttggtcgaga agccggcata aggaccgaac
     7741 agcagcgagg tcttgccatc taccacgcgg gtatccaggt gcggtaccga catcggcggc
     7801 gagccgaccg ctgcttggct gtagaccttg gcctggtgct gcttgaccac gtccgggttg
     7861 tcgcaacgca gccattggcc gctgaccggg aagccaccaa agcctttgcc ttccggaatg
     7921 ccggacatct gcagcagcgg cagcgccgcg ccacctgcgc cgaggaagac gaacttggcc
     7981 tgaatttcac ggctgctgcc gttctgctga tctttgacgc tgacgcgcca gccatcggca
     8041 ttgcgcttga ggccgacaac tttctggctg caggtgactt tggcgtctgc ctgttggccg
     8101 aggtggtcga gcaggtgcgt ggtcagagtg ccgaagttga cgtcggtacc ggccatgacg
     8161 cgggtggcgg caatcggctc gttcgggtca cgacccggca gcatcagtgg catccactcg
     8221 gccatggtgg cgcggtcttc ggtatattcc atctcggcga aagcgtggtg cccggtcagc
     8281 gcgtcaaagc gcttcttcag gtagtcgata cctttctgac cgcgaacgaa gctcaggtgc
     8341 ggaaccggat tgataaacga ctttggcgaa ccaaagcggc ctttctcaac caggtaggcc
     8401 cagaactgct tggattcttc gaactgggtg ttgatggtca ccgacttctt gatgtcgatc
     8461 gaaccgtccg ccgcttccgg cgtgtagttc agctcgcaca ggccagcgtg gccggtgccc
     8521 gcgttgttcc acggattgga gctttcaatc gctccggagg ccatcatctc gagcacttcc
     8581 agcttgatcg ccgggtcgag ctctttcagc agtacggcca gggtcgcact catgatgccg
     8641 gcaccaacca gcaccacatc cattgtttcg taatcgtttt gcgccattac cacttctccg
     8701 caaaccagca ctcaatagac gttaggcagc ttgtgtatgc acacagccaa tcagcagtca
     8761 gcgagacgct cagggttgaa ggaagcaggc ttctgggtgc agatgccaac cgcgagatcg
     8821 ttcattttcg ccacactctt gtgaagtttt ccaaaccgtt tttttcacgc tcttttgtag
     8881 tcgtgaacct caaaacacgt ctgcctgggc cgacttttcc acgacgtaaa gcgaccattg
     8941 cagcaactgc atggcgtttc acagtgggaa aaagcggcag ccggaaggca gcgcgaagtg
     9001 ggcgactctc tggggcgcgg ccgatgaaat ggtgcatcag cgtgacggca gggcccgaac
     9061 aggaggcgtc cttataatcg gggcgcgatt ataagggcaa agcaggaaaa ttgatcgcct
     9121 ggcgtgactt ttcttcttcc gccggtcagg ccgccagcgt ttggtgcggg cgcaggcatg
     9181 gcgtgacctg cacggttctg acgccggctg gcagcggtcg attgagccag ctcatgcagc
     9241 tgtgttgcac ctcaatccag ccggagcctt gcggcgcctg ggctgactgg cgcaaggcgc
     9301 ggcagatgcc ttgcgcatcg accagggcaa atgtgcgcat cgggcggcgt ggggcgagta
     9361 ggttgcgcag tgagaacatg gcgaatcact catgagcagg gaagactaaa gtcttgtgcc
     9421 tgacgtgcat gacaagggcg ttacgggaac cagttgtccc cttaaacgtc ctaacaacca
     9481 gcgctgtcag gcatttaccg cgctggttaa actgcgcgcc acttctaccc cttatctatg
     9541 gagagttgag catgctgcat cgtctgctgt ttggtctggt ggctgtcgcc agccttactc
     9601 tggtgggttg tgcccacagc ccgcaacaac tcagcccaca acccaaactc aatagcccgc
     9661 tgactgccgt tggccagggc cagccggtgg tggtgcgggt cgccgatggt cgtccttcgc
     9721 ccgtattggg cacccgtggt ggtctctatc cggaaaccag cgccatcagc gtgacgggtc
     9781 aggacatcct gcccaagctg caggcccagg ctgaagcggc cgtgcgtctg ctcggcttta
     9841 ccccaagcgc aaacgcctac aacgcgccac agctgaccct gaccctggcc gaactgaagt
     9901 atcagtcgcc taaagaaggg atgtacgtaa ccgaagctga catgaccgcg accttccgcc
     9961 ttgatgtgca aaacagtagc cgtcgttaca gcggccgtta cggcgcgtcg ctgaaccagc
    10021 gcttcggtat ggcgccgaat caggcgacca acaccaagct gatcagcgat gtgttgagcg
    10081 atgccctgac ccgcgccttc aaggatccga ccatcagcca gatgctcgcg cagtaagctt
    10141 tggcgctgta aaaaagcctg gattattcca ggctttttta cagctgcgtt ttgcagcctt
    10201 ttaggccgct tcgctgtaga gctcgggcag gctcatgccg gtgcgcgtgt ctatgtcggg
    10261 cagggcgtaa tgctcatggc cgcccatggc gcagtacacc agccagtggt cgtcctgcac
    10321 gttgaaagac agcccgtcga tgacggcgtc tatgtcgtcc agcgaatcaa tcaggtagtc
    10381 gcggtcttgc ggttgggcat gcagcatgcg gtgttcctcc atggatgttt aaggcctgtc
    10441 accatgctcg aagccttttg gccaaagcgt tgggacgagt gttagaccat agttcacgca
    10501 aatgacagtg tttagaccgc ttggtcatag tggctgcgat tcgtcggaat cgaaccgcga
    10561 gtgcggcaac cttcggtaga ctccgcggcc gtatttggac tgcccgagac tgcatcgggt
    10621 gtgctgataa tcaggcgcga cgttgatcgc atgccttata gaagtagagg ttgagcatgt
    10681 cgttgcaagc gctctggttg ctgatcgaag gtcagtctgg gcagtggctt aatgggctgg
    10741 cgctgttctt tgcactggcc ggcgcctggc tgttactggc cacccgtatg cgcgagcagc
    10801 gtgcccgggc gcggttgctg gccggcaatc agttgaatga actggcggat gaggcctatg
    10861 cctttgatga gccgacgctg cgcctgaacc gcttctttta ttgcttcggt catgcctgcc
    10921 tggcggcggc gttgctgctg tcctggctta gcacccagct gtaacccggt tttctcaggc
    10981 aataaaaacg gcagccaatg gctgccgttt tttctggctg cgctttgcct acagcggcag
    11041 gccggccttg atccggtact ggttacgcac cgggttggcg tattgcagga tcaggtaggg
    11101 ctgttgctcg ctcgggcagt tggccaggcg ccgctgccat tcgctcttgg cggcgctgag
    11161 ctctgcggcg gggaatagct ctgcagcgct gggcacggcc agctccgggt cgcggtcagc
    11221 ccacatggca taggccaggt aatgcacggg aaacagccga taaccgctga gaatctgccg
    11281 gtccatctcc gccgccagca gtttgctgtc ttctaggcct tggctgaccg gcgcgccgaa
    11341 atgtatatgc acgcggcctt tgtaaccggt gatgcccagg gcgatgctct ggtcatcctc
    11401 gcccgctgcc ttggtgtagc tgccactgct ggcgcggata aacagctcgc gcgccttggc
    11461 ctggtcgcag ggatcgtact cgtagctgat cgataccggc gtcaggcgca gggcgttgat
    11521 cacctcggcg aacggttcgc ccttgcggct catatggaac atcttgagga ttgccgaatc
    11581 ggtgcggtca tcaccatcct tggcgcggcc ttcggcctgg gcgatccaga tcgactcgcc
    11641 atcattgcgg atcgagtgat tgatataggc cgatagcagc tggtaagccg ccagcttctc
    11701 gcgccgcccg ctgatcgaac ggtgcacgat aaagctcttg ttcaggcgca tcaggtcgct
    11761 gacaaacggc ttctgcagca ggttgtcgcc aatcgcgatg cgcggcgtcg gcaggccagc
    11821 gtggtacacc gcgtagttga cgaacgccgg gtccatgacg atatcgcggt ggttggcgag
    11881 gaacagatag gcgttgccgg cctgcaggtg ttcgatgccg gaatagctca cgccatcggt
    11941 ggcctgctca atggtgcggt cgacatagct ctcgatgcgc gtctgcaatt gcgccaccga
    12001 actcacgtcg cgaaactcaa gacgcaggcg ataggctata agtggtttaa gcagccagcc
    12061 taaaggtgcc gccaggcgcg ggaagcggaa ctgagtgagg atgccgagaa acgcatcatc
    12121 ggccagcagg cgcgccaaca cggcagggac ttcggcgtcg gcgtaaggtc ggatggcatc
    12181 gaattcgccc atcatgctct cttgttgtgg ttggctgtgg agatgttcac tgcgctggtc
    12241 gttgcgcgtg acaatggata gtgcgcgact gcagggccag ctatagaccg gcgattatac
    12301 gcccaagtca caaggagaca gcgatgctgg aaagccagga ctaccaatgc ccttattgcg
    12361 gcgagcaggt tgaggcgctg ctcgaccttt ccgccggcga tcagcagtac atcgaagact
    12421 gtccggtgtg ttgccggccc atcgtgtttt cgctgcagac cgatggtgag gactggagcc
    12481 tggatgtgcg cggggagaat gactgatgca gcgaatctac gagccgcagg acctgatgga
    12541 aggcgagttg ctgctggcta tgctggccag cgagggcatc gaagcctacc tcactgggcg
    12601 gcatttggct ggtgcagtgg gtgagttacc tgcctgcggt ttgctcggag taatggttga
    12661 ggatgagcag gcgcacagcg cacaacagct gatcgccgcg tacaatgccg cgctgccgct
    12721 gcccggtgat gagccggata gcttccaagg tacgttgctc tgctaagagc ctgttacggt
    12781 ctgcggcgcg tcggccctgc tgcctttatt tcgagtgtta tcgagtttcc catgtgcgga
    12841 cgttatgccc tgttccgttg gacgcctgca tttgcggcga ttcccggttt tcccgccgat
    12901 cagttaccgc actggagcat ttcgcccaat agccaggtgc tgctgctgcg caacgtcgag
    12961 ggcgagcgtc agctgacgcg cgcgcgttgg gggctgacgc cgccctggtt gactgatctg
    13021 accaagaccc cggctcaagc ccgtgcggaa accttggctg agcagccgat gtttcgtgag
    13081 gcgtttcgcc tgcgtcgcgg tctgctgcca gccaacggct tttacgagtg gcggggcacg
    13141 acgcgcaaac gcccgtactg gctcaccggt gagaccacac cgctgtattt cgccgctttg
    13201 tgggaagcct atccggttga gggttgcgtc tacctgagtg ctgcggtggt gactcaggcg
    13261 gcggccagtc agcgtcggcc gctgatcctc gatgaagccg ggcaggccgc ctggttggct
    13321 gccgacaccc cggcggccga cttgcaggct ttattggcga gtccgccgcc ggcgctgcgc
    13381 gagcgggtgc tggcgaatct gatcaatgat cccaagctgg atgcgcctga gtgcctgacg
    13441 ccggcctagg ccagttaaag ggcgc
//
LOCUS       DASRUM010000206         8268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2272346, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000206 DASRUM010000000
VERSION     DASRUM010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8268
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2272346"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1348
                     /locus_tag="VFF22_13610"
     CDS             17..1348
                     /locus_tag="VFF22_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HZX17883.1"
                     /translation="MEAHLTEWLNLGIRWIHMITGIAWIGASFYFVWLENNLNRSNPR
                     EGLSGDLWAIHGGGIYHLEKYKLAPPKMPENLHWFKWEAYFTWLSGVALMLVVYYLNP
                     SLYLVKPGVDLDPALAIAIGFGSMVAGYVAYHFLCDSPLGKRPALLGAVLFVLIIAAA
                     YGFSQIFSGRAAYIHVGAIIGTIMVGNVFFTIMPAQRALVKAIEDNTTPDPTLPAKGL
                     LRSRHNNYFTLPVLFIMISNHFPSTYGSQYNWLILAGIAMLAVLVRHYFNTRHDSSKY
                     IWTLPAAALGMICLAFVTGPSMKAAAPQEPQAAVIKYAPVPATGISGHPEVVAKAADP
                     AAKPAETAAAPEASPPAEAAAGAGDFAQVNSVIQERCSVCHSATPSSPMFTAPPAGFI
                     LDTPEQIKAQAAKIHAQSVASQIMPLGNITQMTQEERELIGSWIDQGAQLN"
     gene            1691..3187
                     /locus_tag="VFF22_13615"
     CDS             1691..3187
                     /locus_tag="VFF22_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HZX17884.1"
                     /translation="MTTTPSTETTAAPVANNDLIYQLHDTPAFAPAIFAALQHVLASF
                     VGIITPTLIVGGVLGLGAYVPYLVSMALFVSGLGTFIQAKRIGPVGSGLLCLQGTSFG
                     FLSVILSAGFIVKGRGGSEEEILSTIFGVCFCAAFVEIAFSQFINKLRRVITPVVTGT
                     IICLMGLSLIKVAMTDIAGGYGAADLGALHHLGLGGLVLVTIVILNRFPSQVVRLSAV
                     IIGLVLGFSVAWFTGKVDFANMAQVPLISVPVPFKYGFNFDWVAFVPIAVIFLITPLE
                     TAGDLTANSIISKQPVKGPLYMRRIKSGILADGCNSAMAAMFNSLPMTTFSQNNGVIQ
                     LTGVASRHVAFYIAGILVLLGLFPAVGAVLQLMPKPVLGGATLIMFGTVAVAGIKILT
                     EAGLHRRNVLIVAISLGLGLGVAAVPEVLSQMPDALKNIFGSPITIGAFSAILLNIFL
                     PEEHLQLEENDYDPEAHLHTVLQNPQDDSAVDAPDSSRNELNVAPRTS"
     gene            3270..4142
                     /locus_tag="VFF22_13620"
     CDS             3270..4142
                     /locus_tag="VFF22_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HZX17885.1"
                     /translation="MKLKHLPHGIALSAGLFSGQQALASDAFLWQDTSLSYLYGSNFQ
                     RLNFNEGEQRSQTTFSLENASGWSWGDTFFFLDYIDADNEQARGSNFGNLDIDEKSNF
                     YYMEFSPRVSLSWLTGQDLSAGPLKDVYAAFTYEKGNGGPGTENYLYGIGTAWNAPGF
                     AYLNANLYAVKVNNHIFFDHDFKASEKGNGHTYQLTVNGAYPFAIGEQDFVIDGFIDW
                     RAPSKDANTQTSVGSSIQIKWDAGKALFGEGRKLYVGTELNMWHNKYGIKPIDGSTDG
                     FDQTAVQALVKYHF"
     gene            4573..6030
                     /locus_tag="VFF22_13625"
     CDS             4573..6030
                     /locus_tag="VFF22_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017246514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HZX17886.1"
                     /translation="MPTDDFRIKQIDTSLHNEDLAPLPPAQRKWGWFEIFNVWSNDIQ
                     SLFGYTLAATLFISYGLNGWAVLAGIVLAGFIVMGLVQLTGKPSVKYGIPFPVMARAS
                     MGVRGANFPAVVRGIVAIFWYGVQTYFASTAVALLLNSLFGSGSSATFLGMTAIGWLS
                     YVIVCVFQVALFIRGIDWITKFLNWAGPLVYLVMIALMLMIWYQAGPSLLSELGEIFR
                     GTGEYAAGPVAAFVAVVGTMVAYFAAVVINYGDFARFVKSEKQMTVGNFLGLPVSLAI
                     FSMIALVITAGTVVVFGETLTNPTDIVARVDNLTLTIIAALTFFAATVGINLVANFIP
                     PAYDIANLAPAHISARTGGFITAAIAFFIGALWVSFISNVGIAAFVDTLGAVLAPLYG
                     ILVADYYLVRRQQLNLQDLFSAEPGSTYYYNGGWNRNAMIAFGVASLFSVASVWTPGL
                     NELSGYSWLFGAMLGALFHYVLMRKHCAVKTPQPA"
     gene            complement(6074..6892)
                     /locus_tag="VFF22_13630"
     CDS             complement(6074..6892)
                     /locus_tag="VFF22_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017679205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX17887.1"
                     /translation="MKPSHVCSLFAVVCSSAVSAESYVIGVEKLAFAPHYSIDAQGHY
                     QGFARELFDLFAQTSGVELSYKVLPVEQLLPALLSGQVDFKYPDSETWAQEQKAGKTL
                     SYSQGVVEYVDGVLVAPQRHGQPIEQIKRLAMVNGWTPWGYQERIAAGQIELTYNDDL
                     RQMIRQALKKDTDGAYFNVVVATHYLDNIRARPGALIFDAQLPHNRGSFKLSTLKHAA
                     FIQRFDRFLLDEQVAIAELKSRYRVEANLDSAHIGVEQWKLDFLKRQQERAASQ"
     gene            complement(6965..7978)
                     /gene="moaA"
                     /locus_tag="VFF22_13635"
     CDS             complement(6965..7978)
                     /gene="moaA"
                     /locus_tag="VFF22_13635"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HZX17888.1"
                     /translation="MSSRRHVVSNELVDPFGRRITYLRLSVTDRCDFRCTYCMSEDMV
                     FAPREQILSLEELYAVADAFIGLGVKRIRITGGEPLVRKNLLSLLTRLGQRPELEDLA
                     ITTNGSQLADLAPQLRAAGVKRLNVSLDSLQRERFAAFTRRDKLDQVLEGIEVARAAG
                     FRKIKLNAVVQKGRNDDEVLGLVEFAMARGLDISFIEEMPLGSISSHQREQTFCSSDE
                     VRERIEQRYALVRSSKLTGGPSRYWQVVGSDTQVGFISPHSHNFCGDCNRVRVTAEGK
                     LVLCLGHENALDLKGLMRAHPGDSQRLSEALMQALQLKPERHHFETDAQVQVIRFMSM
                     TGG"
     gene            8174..>8268
                     /locus_tag="VFF22_13640"
     CDS             8174..>8268
                     /locus_tag="VFF22_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZX17889.1"
                     /translation="MTSIRERNRELILRAASEEFADKGFAASKTS"
BASE COUNT         1686 a         2590 c         2286 g         1706 t
ORIGIN      
        1 gaaagaggta ctgattgtgg aagcgcattt gactgagtgg ctgaacctgg gtatccgctg
       61 gatccatatg atcaccggca tcgcctggat cggcgcatcc ttctatttcg tctggctgga
      121 aaacaacctc aaccgcagca acccgcgtga agggttgtcc ggtgacctct gggcgattca
      181 cggtggcggt atctaccacc tggaaaagta caaactggct ccgccgaaaa tgccggagaa
      241 cctgcactgg ttcaaatggg aagcctattt cacctggctg tcgggcgtgg ccctgatgct
      301 ggtggtctac tacctcaacc cgagcctgta cctggttaaa cccggcgtcg acctggatcc
      361 ggcattggcc attgccatcg gtttcggttc gatggtggcg ggctatgtgg cgtatcactt
      421 cctctgcgac tcgccgctgg gcaagcgccc ggccttgctt ggcgccgtgc tgttcgtgct
      481 aatcatcgcg gccgcctatg gcttcagcca gattttcagc ggccgcgctg cctatattca
      541 tgtcggcgcc attatcggca ccatcatggt cggcaacgtg ttcttcacca tcatgccggc
      601 ccagcgcgcg ctggttaagg ccatcgaaga caacaccacg ccggacccga ccctgccagc
      661 caaaggcctg ctgcgttcgc gccacaacaa ctacttcacc ctgccggtgc tgttcatcat
      721 gatcagcaac cacttcccga gcacctacgg cagccaatac aactggctga tcctggcggg
      781 tatcgcgatg ctggcggtac tggtacgtca ttacttcaac acccgtcatg acagcagcaa
      841 gtacatctgg accctgcctg ccgctgcatt gggcatgatc tgcctggcat tcgtcaccgg
      901 cccgagcatg aaggccgctg caccgcagga gccgcaagct gcagtaatca agtacgcccc
      961 ggtaccggcc actggcatca gcggccatcc ggaagttgtt gccaaagctg ccgatccagc
     1021 cgccaagccg gcagaaaccg cagcggcccc tgaggccagt cctccagcag aagccgcagc
     1081 tggcgcgggc gactttgccc aggtcaacag cgtgattcag gaacgctgca gcgtgtgcca
     1141 ctcggccacg ccaagcagcc cgatgttcac cgcaccaccg gctgggttca ttctcgatac
     1201 gcctgagcag atcaaggcac aagccgcgaa gatccacgcg caaagcgttg cttcgcagat
     1261 catgccgctg ggcaacatca cccagatgac ccaggaagaa cgcgagctga tcggctcgtg
     1321 gatcgaccag ggcgcacagc tcaactgatc caattaaatg ccgcgcccat caggacgcgg
     1381 catttgttta tctgcctgcg aaactcgcta ctcgacccct cccccggttc aaaagcctta
     1441 acaggcttta acctagaagt ccccctcgaa acaaacccct cacatagcta agtgcgagct
     1501 gttcagctat ctgactgacg gttcattttt tgtgcacaat tttaaaataa attgttttta
     1561 tcgctgtgaa caagttgcta tagtcgatcc attctgacca ggcggacaac ttcttcccag
     1621 gtacagagca gaggcgccgt gaagcctcgc caagcccatg accgtataaa aacaactggc
     1681 aggctctgat atgaccacga ctcccagcac cgagaccaca gctgcgcctg tggcaaacaa
     1741 cgacctgatc taccaactgc acgacacccc agcctttgct ccggcgatct tcgccgcgct
     1801 gcagcacgta ctggccagct tcgtcggtat catcacccct accctgattg tcggtggcgt
     1861 cctcggcctc ggcgcctatg tgccctatct ggtcagcatg gcgctgttcg tatccggcct
     1921 cggcaccttt atccaggcca agcgcattgg cccggtcggt tccggcctcc tgtgcctgca
     1981 gggcaccagc ttcggttttc tcagcgtgat tctcagcgcc gggtttatcg tcaagggccg
     2041 tggcggcagc gaggaagaaa ttctctcgac gatctttggc gtgtgtttct gcgcggcctt
     2101 tgtcgagatt gccttcagcc agttcatcaa caagctgcgc cgcgtaatca ccccggtcgt
     2161 taccggcacc atcatctgcc tgatgggctt gtcgctgatc aaagtggcca tgaccgatat
     2221 cgccggcggc tacggcgcgg cggatctcgg cgccctgcac cacttgggcc tcggcggcct
     2281 ggtgctggtg accatcgtta tcctcaaccg cttcccgtcg caggtggtgc gcctgtcggc
     2341 cgtgatcatc ggcctggtac tgggctttag cgtggcctgg ttcaccggca aggtggattt
     2401 tgccaacatg gcccaggtgc cgctgatcag cgtgccggtg ccgttcaagt acggcttcaa
     2461 ttttgactgg gtggcgtttg tgccgattgc ggtgatcttc ctgatcaccc cgctggagac
     2521 cgcaggcgac ctgacggcca actcgatcat ctccaagcag ccggtcaaag ggccgctgta
     2581 tatgcgccgg atcaaatccg ggatcttggc cgatggttgc aactcagcca tggcggcgat
     2641 gttcaacagc ctgccgatga ccaccttcag ccagaacaac ggcgtgatcc agctgaccgg
     2701 tgtggccagc cgccatgtgg ccttctatat cgccggcatc ctggtgcttc tgggcctgtt
     2761 cccggcggtg ggtgcggtat tgcagctgat gcccaaaccg gtactcggtg gcgccacgct
     2821 gatcatgttc ggcaccgtgg ccgtggcggg tatcaagatt ctcaccgagg ccggcctgca
     2881 tcgccgcaac gtgctgatcg tggccatctc cctgggcctg ggcctcggtg tagcagccgt
     2941 accggaagtg ctctcgcaaa tgccggatgc gctgaagaac atcttcggtt cacccatcac
     3001 cattggtgcc ttcagcgcca ttctgctgaa tatcttcctg ccggaagaac atctgcagct
     3061 ggaagaaaac gactacgacc ccgaagcgca cctgcacacc gtgctgcaaa acccgcagga
     3121 cgacagcgcc gtagatgcgc ctgacagcag ccgcaacgaa ctcaacgtag ccccgcgcac
     3181 cagctgaacc accgcccctt tgcctgacag atcctgcggt tcgccgcagg ttcgagaaac
     3241 cttgtaagtc caataataag gaaaccatga tgaaactgaa gcacctaccc catggcatcg
     3301 ccctctcggc tggactgttt tccggtcagc aggccttggc cagcgacgcc tttctctggc
     3361 aagacaccag cctctcctac ctctacggca gcaacttcca gcgcctgaac ttcaacgaag
     3421 gtgagcagcg ctcgcaaacc accttcagcc tggaaaacgc cagcggctgg agctggggcg
     3481 acactttctt cttcctcgac tacatcgacg ccgacaacga gcaggcgcgc ggtagcaact
     3541 tcggcaacct ggatatcgac gagaagagca atttctacta catggagttc tctccacgcg
     3601 tcagcctcag ctggctaacc ggtcaggacc tttccgcagg cccgctgaag gatgtgtacg
     3661 cagcctttac ctatgaaaaa ggtaacggcg gccctggcac cgaaaactac ctgtacggca
     3721 tcggcaccgc ctggaacgct ccaggcttcg cctaccttaa cgccaacctg tatgcggtga
     3781 aggtcaacaa ccacatcttc ttcgatcatg acttcaaggc ttcggaaaaa ggcaacggcc
     3841 acacctacca actgactgtg aacggtgcct atccgtttgc catcggcgaa caagacttcg
     3901 tgatcgacgg ctttattgac tggcgcgcgc cgtccaagga cgccaacacc cagacctcgg
     3961 tcggctcttc gatccagatc aaatgggatg cgggcaaggc gttgttcggt gaaggccgca
     4021 agctgtatgt cggtaccgaa ctgaacatgt ggcacaacaa gtacggcatc aagccaatcg
     4081 acggttcaac cgatggcttt gatcagaccg cagtacaagc gctggtgaag taccacttct
     4141 aagcgaactc cagtgctgcc gggtaggcgg cagcgctaag gtaaaggggg gcacgaataa
     4201 cgtgccccct ttttttgccc cgaaataagg cgcaccgagc gctgctgctg gaactttgtg
     4261 ccaattgctt cgacaagccg ccccgggcga gaaaaaacca tggccgacaa ctggttaagt
     4321 tgtccaccca agcactaaaa ccgaccacct agtcaatatc tagagcacat caaaaggcta
     4381 atttgtacac aactttgtaa aaataagtgt taacaattaa attttatgtg tacaacaata
     4441 ttgcctacaa cctgctctat gcgattaacg gcccgaatca ccaaggccgg ccaaaagcgg
     4501 aacagatagt gcatacaatc ctgctcagac atgcgccgac agacgcacat aacaagactt
     4561 gaggagctgc tcatgcccac cgatgatttt cgcatcaagc aaatcgacac ctcgctgcac
     4621 aacgaggatc tggcgcctct gccgccggca caacgcaaat ggggctggtt cgagatcttc
     4681 aacgtctggt ccaacgacat ccagagcctg ttcggctata ccttggctgc caccctgttt
     4741 atctcctacg gcctgaacgg ctgggcggta ttggccggca tcgttctggc cggctttatc
     4801 gtcatgggtc tggtgcaact gaccggcaaa cccagtgtca agtacgggat cccctttccg
     4861 gtcatggccc gcgccagcat gggtgtgcgc ggggccaact tccccgcggt ggtgcgcggt
     4921 atcgtggcga tcttctggta tggcgtgcag acctatttcg cctccaccgc cgtggccctg
     4981 ctgctcaact cgttgttcgg cagtggttca agcgcgactt ttctgggcat gacagccatc
     5041 ggctggctct cctatgtgat cgtctgcgtg tttcaggttg ccctgtttat ccgcgggatc
     5101 gactggatca ccaagttcct caactgggcc ggcccgctgg tctacctagt gatgatcgcg
     5161 ctgatgctga tgatctggta ccaggccggc cccagcctgc tcagcgagct gggcgagatc
     5221 ttccgcggca ccggcgaata tgctgctggc ccggttgccg cctttgtcgc ggtggtcggc
     5281 accatggtcg cctacttcgc cgcggtggtg atcaactacg gcgacttcgc ccgcttcgtc
     5341 aaaagcgaaa agcagatgac cgtcggcaac tttctcggtc tgccggtgag cctggcgatc
     5401 ttctcaatga tcgccctggt gataaccgcc ggtaccgtag tggtgttcgg cgagaccctg
     5461 accaacccaa ccgatatcgt cgcccgggtg gataacctga ccctgaccat catcgccgca
     5521 ctgaccttct tcgctgccac ggtggggatc aatctggtgg ccaactttat cccgccggcc
     5581 tacgacatcg ctaacctggc cccggcccat atcagcgcgc gcaccggtgg ttttatcacc
     5641 gcggccatcg ccttctttat cggtgcgctg tgggtgtcct ttatcagcaa cgtcggcatt
     5701 gccgccttcg tcgacaccct gggcgcggtg ctcgcaccgc tgtacggcat tctcgtcgct
     5761 gactactacc tggtgcgcag gcagcagctc aacctgcagg acctgttcag cgccgaaccc
     5821 ggttcgacct actactacaa tggcggctgg aaccgtaacg ccatgatcgc cttcggcgtg
     5881 gcctcgctgt tctcggtcgc ctcggtatgg accccgggcc tcaatgagct gtccggctat
     5941 tcctggctgt tcggtgccat gcttggcgcg ctgttccact acgtgctgat gcgcaaacac
     6001 tgtgccgtga aaacccctca gcccgcgtag ctgagcccgc ccctggacgc cgggattgcc
     6061 ggcgtctagc ccatcactga ctcgctgccc gctcctgctg gcgcttgaga aagtccagct
     6121 tccattgctc cacgcctata tgcgccgagt cgaggttggc ctcgacccgg tagcggctct
     6181 tcagctcagc gatggcgacc tgctcatcca gcaaaaagcg gtcgaaacgc tgtatgaaag
     6241 ccgcgtgctt gagggtcgac agcttgaagc tgccacggtt atgcggcaac tgcgcatcga
     6301 agatcagcgc gcccggtcgt gcccggatgt tgtccagata atgagtcgcc accaccacgt
     6361 tgaagtaagc gccgtcggtg tccttcttca gtgcctgacg gatcatctgg cgcagatcat
     6421 cgttgtaggt cagctcgatc tgtccggcag cgatacgctc ctgatagccc cagggcgtcc
     6481 agccattgac catggccaga cgcttgatct gctcgatggg ctggccatga cgctgcggcg
     6541 cgaccagcac gccatcgacg tactcaacta caccctgact gtagctcagg gtcttgccgg
     6601 ctttctgctc ctgcgcccag gtttcgctgt cggggtattt gaaatccact tgaccgctga
     6661 gcagcgcagg caacagctgc tccacgggca gcaccttgta gctcagctcc actccgctgg
     6721 tctgcgcgaa caggtcgaac agctcacggg caaagccctg gtaatgacct tgcgcatcga
     6781 tgctgtagtg cggcgcaaag gccagcttct ctacaccaat cacatagctt tcagcgctga
     6841 ccgcgctgga acagacaacg gcgaacaaac tgcagacgtg cgagggcttc acagcaaacc
     6901 tcctgcttaa ttatgagagt ggaacgaatg gaaccgccca aggcggtgag gaagcggcta
     6961 ggggctagcc accggtcatg ctcataaagc gaataacctg cacctgagca tcggtctcga
     7021 agtggtgacg ttcgggtttc agttgcaagg cttgcatcaa ggcttcactc aagcgttggc
     7081 tgtcgccggg gtgggcacgc atcaggcctt tcaggtccag ggcgttttca tggcccagac
     7141 agagcaccag cttgccctcg gcggtgaccc gcacgcggtt gcagtcgccg cagaagttgt
     7201 ggctgtgggg cgagataaaa ccaacctggg tgtcgctgcc gaccacctgc cagtagcgcg
     7261 acggcccgcc ggtcaacttg ctgctgcgca ccagggcata acgctgttcg atgcgctcgc
     7321 gcacctcgtc actggagcag aaggtctgct cgcgctggtg gctggaaata ctgcccagcg
     7381 gcatttcttc gataaagctg atgtccaggc cccgcgccat ggcgaactcg accaggccga
     7441 gcacttcgtc atcgttgcgg cctttctgca ccacggcgtt gagtttgatc ttgcgaaaac
     7501 cggcagcgcg cgccacctcg atgccttcca gcacctgatc gagcttgtcg cgccgggtaa
     7561 aagcggcaaa acgttcacgc tgcagggaat ccaggctgac attcaggcgt ttgaccccgg
     7621 ccgcgcgcag ttgcggcgcc aggtcagcca gttgcgagcc attggtggta atggccaggt
     7681 cttccagctc tgggcgctgg cctaggcggg tcagcaggct gagcaggttc ttgcgtacca
     7741 gcggctcgcc accggtgatg cggatacgtt tgaccccaag gccgataaaa gcgtccgcca
     7801 ctgcatagag ctcttcaagg ctgaggatct gctcgcgcgg ggcgaacacc atgtcctcgc
     7861 tcatgcagta ggtgcagcgg aaatcacagc gatcggtaac cgataaccgt agataggtga
     7921 tgcgccgacc gaaggggtca accaattcat tgctcacgac gtgtctcctg gagctcaacg
     7981 agccgagact ttttgttatg aatgcatagg gcaagataaa aacagaaaaa ttaaattttg
     8041 tatacaacaa cctgacctgc aagtcagata gcccgccaga gcccaggcca caagccttga
     8101 gcgctgtgca aagccggtgc taccatcccc ggcgctgccc agcgacgggc gttcgacaga
     8161 agcaaagagg cttatgacca gtattcgcga gcgtaacaga gagctgattt tacgcgcagc
     8221 cagtgaggag ttcgccgaca aaggctttgc tgccagcaaa accagcga
//
LOCUS       DASRUM010000207         2780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2272959, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000207 DASRUM010000000
VERSION     DASRUM010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2272959"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1168
                     /gene="glnA"
                     /locus_tag="VFF22_13645"
                     /pseudo
     CDS             77..1168
                     /gene="glnA"
                     /locus_tag="VFF22_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
     gene            1343..1897
                     /locus_tag="VFF22_13650"
     CDS             1343..1897
                     /locus_tag="VFF22_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HZX17890.1"
                     /translation="MRPLLICLLLGLALPASAQIYKYTDANGNTVFTDQPPEGQATQN
                     VELPKTNSVSMPVPSLPTATTPEPADSAAPYSTLQLTGIPDDEAMRANNGTFSVGVDI
                     QPRLANNHRLRLLLDGKPYGQPSNVPRLQVSNADRGEHSLAVEVLSGEQSIQQSATAT
                     FTVQRVNTSSPALRPPPPKPKPAP"
     gene            1900..2517
                     /locus_tag="VFF22_13655"
     CDS             1900..2517
                     /locus_tag="VFF22_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HZX17891.1"
                     /translation="MRNLLLCGLMLLALPAVAQVYTYIDAEGNRVFTDKPRSGDAERV
                     ELAPSNSMPTIQTQTAPVTEAPPEPSQRYSLLRVLVPQPDATIRDSAGNLIVSVNSEP
                     KLFPQHSYRLLLDGVQVGEAGSSPVFPLENIDRGTHQLAVEIIDQQGRIIERTPTQPF
                     HMLRISLAQKRMVNPCKKADYGVRPECPLKDKPPEKKDIPLVPFI"
BASE COUNT          641 a          922 c          707 g          510 t
ORIGIN      
        1 caagtttctg agcgtgttct tgcagcaaag agtccatagg taatagtctg tgaataaagg
       61 tctgcaaagt cctctctcga tcgccaagcg cgctgaagag ttcctgaaaa ccactggtat
      121 cggtgacacc gtattcgtcg gtccagagcc tgagttcttc atcttcgacg aagtgaagtt
      181 caagtccgac atctccggct cgatgttcaa gatcttctcc gagcaggcgt cctggaacac
      241 cgactctgac atcgaaggcg gcaacaaggg ccaccgccca gccgtcaaag gtggctactt
      301 cccagttccg ccgtgcgacc acgaccacga aatccgtacc gccatgtgta acgccatgga
      361 agaaatgggc ctggtcatcg aagttcacca ccacgaagtg gcgactgccg gtcagaacga
      421 aatcggcgtg aagttcaaca ccctggttgc caaggctgac gaagttcaga ccctgaagta
      481 ctgcgtacac aacgtggccg atgcctacgg caaaaccgcg acctttatgc cgaagccgct
      541 gtacggcgac aacggttcgg gtatgcacgt acacatgtcc atctccaaag atggcaagaa
      601 caccttcgct ggcgaaggct atgccggcct gtccgacacc gctctgtatt tcatcggcgg
      661 catcatcaag cacggtaaag cactgaacgg cttcaccaac ccgtcgacca actcctacaa
      721 gcgtctggtc ccaggtttcg aagctccagt aatgctggcc tactcggccc gcaaccgttc
      781 ggcttcgatc cgcattcctt acgtgtcgag cccgaaagcc cgccgtatcg aagcgcgctt
      841 cccggacccg gctgccaacc catacctggc cttcgcagca ctgctgatgg ccggtatcga
      901 cggcatccag aacaagatcc accctggcga tgctgccgac aagaacctgt atgacctgcc
      961 accagaagaa ggcaagctga tcccacaagt gtgcggcagc ctgaaagaag ccctggaaga
     1021 gctggataaa ggccgtgcgt tcctgaccaa aggcggcgtg ttctccgacg acttcatcga
     1081 cgcttacctt gagctgaaga gcgaagaaga aatcaaagta cgcaccttcg tacacccgtt
     1141 ggaatacgac ctgtactaca gcgtctaatc ctgcacgcaa gacaagaggc caccttcggg
     1201 tggccttttt gcgtttggcg acctgcccgt aacacctttt aacctgatga ttgctgcgct
     1261 ggtacacgcc cagccatcaa cgcgcttgcc agcgcgcgct accagtgcaa ggcttagagc
     1321 acatcattag ggagagtcag gcatgcgccc tctacttatc tgcctgctac tgggcctggc
     1381 gttgccggcc agtgcgcaga tctacaaata caccgacgcc aatggcaaca cggtattcac
     1441 cgaccagcca ccggaaggcc aggcgactca gaatgtcgaa ctgccgaaaa ccaatagcgt
     1501 gtcgatgcca gtacccagcc tgcccacagc aacaactcca gaacccgccg atagcgccgc
     1561 gccttacagc acactgcagc tgaccggcat ccctgacgat gaagccatgc gcgccaacaa
     1621 cggcaccttc agcgttggcg tggatatcca gccgcgcctg gccaacaacc atcgcctgcg
     1681 cttgctgctc gacggcaagc catacggcca gccgagcaac gtgccgcgcc tgcaagtaag
     1741 caatgccgac cgtggcgagc acagcctggc cgtggaagtc ctcagcggcg agcaatcgat
     1801 ccagcagagc gccactgcca cctttaccgt gcagcgggtc aataccagca gcccagccct
     1861 gcgcccacct cctccaaaac ccaagccagc tccatgacca tgcgcaatct gctgttatgc
     1921 ggcctgatgc tgctcgctct acctgccgtt gcgcaggtct acacctatat agatgccgaa
     1981 ggtaatcgcg tcttcaccga caaaccgcgg tccggcgatg ccgagcgtgt cgaactggcg
     2041 cccagcaact cgatgccgac catccagacg caaaccgcgc ccgtgactga agcgccacca
     2101 gaacccagcc agcgctacag cctgctgcgc gttctggtgc cacagcccga tgcgaccatc
     2161 cgcgacagcg ccggcaacct gattgtcagc gtcaacagcg agccgaagct cttcccccag
     2221 cacagttatc gcctgctgtt ggacggcgta caggtcggcg aagcaggcag cagcccggta
     2281 ttcccgctgg agaatatcga ccgcggcacc caccaactgg cggtggaaat catcgaccag
     2341 cagggtcgta tcatcgagcg cacccccact cagccgttcc atatgctgcg catctccctg
     2401 gcgcagaaac gcatggtcaa tccctgcaaa aaggccgatt acggcgtgcg cccggagtgc
     2461 ccgctcaagg acaaaccgcc ggaaaagaaa gacattcccc tcgtcccgtt tatctaatct
     2521 cgcgacgcgc accaaatcag tgcgaatatt gcccagcgtt tctatactct ccctgttttg
     2581 ggtcgccacg cgcgacccag ctgcgctgaa aagcagctaa ttcccggcat cccacggcca
     2641 aacacgcttc ttttcggggc tttggtttgc ttcttgcatt ttccagctgc attgctggaa
     2701 cccaagggtc tgctgacatc ccatatgatc atcaacgacg cactgcaccg cctgctgctg
     2761 gacaacctga ccactgccac
//
LOCUS       DASRUM010000208        13458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_766813, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000208 DASRUM010000000
VERSION     DASRUM010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 13458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13458
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766813"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VFF22_13660"
     CDS             <1..991
                     /locus_tag="VFF22_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HZX17892.1"
                     /translation="AAVLGVSSFAQAGATLDAIQKKGFVQCGISDGLPGFSYADEKGN
                     YLGLDVDVCRAVAAAVFGDATKVKYSPLTAKERFTALQSGEVDILSRNTTWTSSRDSG
                     LGLNFAGVNYYDGQGFLVNKKLGVSSAKELDGATVCIQAGTTTELNLSDYFRANGLKY
                     TPITYDTSDESAKSVEAGRCDVLTSDQSQLYAQRIKLADPDSYVVLPEVISKEPLGPV
                     VRQGDEEWFDIVRWSLYAMVNAEELGVTSANVEEQAKSTKNPDIARLLGAEGEFGKDL
                     KLPKDWAVQIVKQVGNYGESFERNVGTGSELKIERGLNALWNKGGLQYAPPVR"
     gene            1093..2277
                     /locus_tag="VFF22_13665"
     CDS             1093..2277
                     /locus_tag="VFF22_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZX17893.1"
                     /translation="MQTTANVPRPGGSVWTDPKARAWLFQILAVIAVVALGWFLFDNT
                     QTNLEKRGITSGFSFLNNSAGFGIAQHLIDYDESNSYGRVFVVGLLNTLLVSVIGIVL
                     ATLLGFLLGVARLSPNWLISKLATVYIETFRNIPPLLQIFFWYFAVMLSLPGPRQSMG
                     VGEAFFLNSRGLYMPAPSPTDSFGLFAGAVIVAIIGVVLLARWSKARFEATGQLFPVS
                     LGVIPLLIGLPGLAILLGGNPLQWSVPELTGFNFRGGWVLIPELMALTLALTIYTAAF
                     IAENVRSGIMAVNHGQTEAARSLGLPAGKTLRLVIIPQALRVIIPPLTSQYLNLAKNS
                     SLAAGIGYPDMVSLFAGTVLNQTGQAIEVIAITMSVYLAISISISMLMNWYNKRIALI
                     ER"
     gene            2287..3384
                     /locus_tag="VFF22_13670"
     CDS             2287..3384
                     /locus_tag="VFF22_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZX17894.1"
                     /translation="MQTHTFKPDLPPPALSVGVVGWLRANLFSSWFNTLLTLFAAYLV
                     WLMLPPLIQWAFIDADWTGTTRADCTSGGACWVFVQQRFGQFMYGFYPSELRWRVDLT
                     LWLAVIGAAPLFVPQMPRKAMYGLAFLVVYPLLAFWLLHGGFFGLSTVSTSQWGGLML
                     TLVIAAVGIAGALPLGILLALGRRSDMPAIRVICVTFIEFWRGVPLITVLFMSSVMLP
                     LFLPEGLSFDKLMRALIGVILFQSAYIAEVVRGGLQAIPKGQYEAAAAMGLGYWRMMG
                     LVILPQALKLVIPGIVNTFIALFKDTSLVIIIGLFDLLNSIKQATTDPAWLGMATEGY
                     VFAALIFWIFCFGMSRYSQHLERKLDTGHKR"
     gene            3394..4155
                     /locus_tag="VFF22_13675"
     CDS             3394..4155
                     /locus_tag="VFF22_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017904195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZX17895.1"
                     /translation="MSEANKQPSAEPMILLQGVNKWYGQFHVLKDINLSVQPGERIVL
                     CGPSGSGKSTTIRCINRLEEHQQGRIVVDGTELTHDLKHIEAVRREVGMVFQHFNLFP
                     HLTVLQNCTLAPMWVRKMPKRQAEEIAMHFLERVRIPEQAHKYPGQLSGGQQQRVAIA
                     RALCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAEDGMTMLCVTHEMGFARTVANRV
                     IFMDKGEIVEQNEPNAFFTNPQNERTKLFLSQIIH"
     gene            complement(4223..4627)
                     /locus_tag="VFF22_13680"
     CDS             complement(4223..4627)
                     /locus_tag="VFF22_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HZX17896.1"
                     /translation="MKRVLTISLLATGLVGCATSAPQLETEHSYQVEWIGERPLIDRS
                     HLTITLGDDGRAYGNAGCNHWFAGYSLEGDKLSFSAAGSTRKMCAPALMEQEARFLDS
                     LSKVERWDFSATEQLQLWPAEGKPLRLWPENK"
     gene            complement(4624..5064)
                     /locus_tag="VFF22_13685"
     CDS             complement(4624..5064)
                     /locus_tag="VFF22_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019410818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17897.1"
                     /translation="MAEHDFRYNLLNPEHTLIECRALAPGRYQVTGNGGSIQSGDTLL
                     VTLKGSRELSMRLQADKVRHLINPRGQWVAVATGPAFKELAIFNWQANCDSCNAQLDF
                     EFAVDAALGKKAQAPAAEARIKELGWQSKNAKHLCPKCINKEAI"
     gene            complement(5092..6084)
                     /locus_tag="VFF22_13690"
     CDS             complement(5092..6084)
                     /locus_tag="VFF22_13690"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HZX17898.1"
                     /translation="MRIILFSSKPYDQQSFSAAGFPDDWQLNFQEAHLRQDSAGLAAG
                     YEVVCAFINDDLSAQVLQQLAAGGTRLIALRSAGYNHVDLHAAHALGLTVVRVPSYSP
                     HAVAEHALALIMTLNRRTHRAFNRTREGDFSLHGLIGFDLHGKQIGIIGAGQIGLTFA
                     RIMTGFGCQLLIHDPTPIAELPAGARQVTLDQLLASSNIISLHCPLNAATQHLINTTR
                     LQQMQRGAMLINTGRGALIDTPALIGALKSGQLGYLGLDVYEEEAELFFEDCSEQPLQ
                     DDVLARLLSFPNVLITAHQGFLTHEALAAIAGTTRDNIAAWLANASINQLLPAA"
     gene            complement(6120..8870)
                     /locus_tag="VFF22_13695"
     CDS             complement(6120..8870)
                     /locus_tag="VFF22_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX17899.1"
                     /translation="MLKDLGIKGRVLMLTLLPTSLLALVLGGYFTWVQLSGLQGQLLQ
                     RGQMIIEQLAPLAAPALHSGDSEVLQRIASQALDQPDVRAVSFLAAERNTLAHAGPSM
                     LNPSPTTGEPQPAQSSNQNATRFLLPVYQQHLSLTGKATEEPGTRLLGWVELELSHHS
                     TLLSGYRSLLASLLLIATGLMVTALLAVRMSRTINDPLRQIKQSVAQLKDGHLETRLP
                     PLGSHELDELASGINRMAESLQNAQEELQHSVDQATEDVRQNLETIEIQNIELDLARK
                     EALEASRIKSEFLANMSHEIRTPLNGILGFTNLLQKSELTPRQQDYLGTIEKSADSLL
                     AIINEILDFSKIEAGKLVLENIPFNLRDLLQDTLTILAPAAHAKQLELLSLVYRDTPL
                     SLIGDPLRLKQVLTNLVSNAIKFTRAGTIVIRAMLEDESADRAQLRISVQDTGIGLSD
                     EDLRELFQAFSQADNSLSRQAGGTGLGLVISKRLIEQMGGEIGVDSTPEEGSEFWITL
                     SLPKARDDAEDLPRPPLLGRRAAALEHHDLARQALQHQLEDCGLQVSGFDNLDSLLSA
                     VAEARHSAQPIELAVLGVSTQTLPPERLDQRIWELERLACKTLLLCPTTEQSLYHSLL
                     PDAYSQLQAKPACTRKLQRGLAELVSPRQNRAENKQSAPSRPPLILCVDDNPANLLLV
                     QTLLDDMGAQVSIANSGYEAVEKAAEQTFDLIFMDVQMPGMDGRQATEAIRQLENERN
                     QGPVPIVALTAHALANEKRALLQSGLDDYLTKPISERQLAQVVLKWTGLALRNQSDEH
                     TGISATHNNLSVLDADEGLRLAAGKADLAADMLSMLLAGLPGDRQAIRQARDSGERNS
                     LIERVHRLHGATRYCGVPQLRAACQRSETLLKQNDPAAQQALDELDAAIDRLAQHAQV
                     SA"
     gene            8988..9701
                     /locus_tag="VFF22_13700"
     CDS             8988..9701
                     /locus_tag="VFF22_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZX17900.1"
                     /translation="MLTCNLPPARILAVEDDPLLASHLHQYLQDSGYCVTLSGDGSEG
                     LHLAADGDFDLVLLDILLPGISGLQALQSLREQRGVPVILMSALGGEQDRIVGFTQGA
                     DDYLPKPFSMDELKVRIEAVLRRVAYERRDLAVLSAQRGGLYFDEQRMDISLAGRWAG
                     LTPAEYRVLELLSRNLDEVLSKPFLYQQALHRAYALHDRSLDMHVSHIRRKLQVLGDS
                     GLRLETVWGKGYLLTGQGL"
     gene            9703..11040
                     /locus_tag="VFF22_13705"
     CDS             9703..11040
                     /locus_tag="VFF22_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase sensor domain-containing
                     protein"
                     /protein_id="HZX17901.1"
                     /translation="MSIPGLHSLFWKLAALLVTFTLLLISLSWFWSQRVELHTSYLSQ
                     PARQQLQAYADAAEQAWLEGGDAGVDKFLQDLHEQEASDALLLDAELRPLGSQVLTPK
                     QRERLTFMRQLDWPMSRRSAELPYINIPFANGEGHLVIQLPERFRPWEYRQPLLWVTR
                     ALLPGMLALLFCIGLYRVLIAPLVRLREQANALHSDDLSARAGAVARRHDELGELGRA
                     FDHMAERLQGTVELQRQLLRDLSHELRTPLSRLRVAAEGGLQGQNLTQRVMHEVQLMQ
                     QLVGDTLELAWLDTERPRLTREQVDLAALWDMLRERACFESGWTPERLVYAVEPGCHL
                     SCNLNALARALENILRNAIRYSPEAGLIRLCGKAQAGEWHLWLEDQGPGVPEQQLAQI
                     FLPFTRLNGARPGDGGFGLGLSIASSSINGQGGRIYAENARPGLRIHLHLPSA"
     gene            11127..12038
                     /gene="cysM"
                     /locus_tag="VFF22_13710"
     CDS             11127..12038
                     /gene="cysM"
                     /locus_tag="VFF22_13710"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HZX17902.1"
                     /translation="MSPHYPTIADCIGNTPLVRLQRLPGNTSNTLLLKLEGNNPAGSV
                     KDRPALSMITRAELRGDIQPGDVLIEATSGNTGIALAMAAAIKGYRMILIMPDNSTAE
                     RKAAMTAYGAQLILVGGMEEARDLALQMQADGKGKVLDQFANGDNPIAHYTSTGPEIW
                     QQTGGQITHFISSMGTTGTIMGVSRFLKEQKPDVQIVGLQPMEGAAIPGIRRWPQEYL
                     PKIYQAERVDRIIDMSQQEAEEVMRRLAREEGIFCGVSSGGSVAAMLRLSQEVENAVM
                     VAIICDRGDRYLSSGVYDAPSTDGKSN"
     gene            12038..13390
                     /gene="rlmD"
                     /locus_tag="VFF22_13715"
     CDS             12038..13390
                     /gene="rlmD"
                     /locus_tag="VFF22_13715"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HZX17903.1"
                     /translation="MAKKDGGLRFQPSGGSRATQVPVGKKQKLTIERLANDGRGIAFS
                     EGRTWFVSGALASEQIEARVLGAHGKVVEARVERIITASEERRNEACAHAKVCGGCSL
                     QHMPHADQLALKQRMLAEQLSRLANLEPQEWAAPLVGPELGYRRRARIAVRWDNKAKR
                     LDVGFRAAASQDIIAISDCPVLVQPLQPILRTLPLVLRELDKPQAIGHVELFSGSSSA
                     VLVRHTAALSEADLARLQAFCTAQNAQLWLHGTAEPQPFEPAQQLSYRLDAFDLDLAY
                     RPGDFVQVNQPVNDAMVAQALEWLAPQADERVLDLFCGLGNFALPLAQRVSELVAVEG
                     VQTMVERAAENAASNGLSNAHFYQADLSNPLAEAGWAQGGFAAVLLDPPRDGALQAVK
                     QMTVLGVKRVLYVSCNPTTLARDSAELLAQGYRLERAGILDMFPQTAHVEAMALFVRQ
                     "
BASE COUNT         2336 a         3853 c         4379 g         2890 t
ORIGIN      
        1 cgccgctgtt ctcggtgtca gcagttttgc tcaagcaggc gctactctgg acgcgatcca
       61 gaagaaaggt ttcgtacagt gcggtatcag cgatggtctg cctggcttct cctacgccga
      121 tgagaagggt aactacctgg gtctcgacgt tgacgtatgc cgcgcagttg ctgccgcggt
      181 atttggtgac gctaccaagg tcaagtacag cccgctgacc gccaaagagc gcttcaccgc
      241 gctgcagtcc ggcgaagtcg acatcctctc gcgtaacacc acctggacca gctcgcgtga
      301 ttcgggcctg ggtctgaact ttgccggcgt taactactac gacggtcaag gcttcctggt
      361 taacaagaaa cttggcgttt ccagcgctaa agaactcgat ggcgcaactg tctgcatcca
      421 ggcaggtacc accaccgagc tgaacctctc cgactacttc cgtgccaatg gcctgaagta
      481 caccccgatc acttacgaca cctccgacga gagcgccaaa tcggttgaag ctggccgttg
      541 cgacgtgctg acctccgacc agtcgcagct gtacgcacag cgcatcaagc tggctgaccc
      601 ggattcctac gtggtactgc cggaagtgat ctcgaaagag ccactgggcc cggtcgtacg
      661 ccagggtgac gaagagtggt ttgatatcgt gcgctggtcg ctctacgcga tggttaacgc
      721 tgaagagctg ggtgtgactt cggccaacgt cgaagagcaa gccaaatcca ccaagaaccc
      781 cgacatagct cgtctgctcg gcgctgaagg tgagttcggt aaagatctga aactgccgaa
      841 agactgggct gtacagatcg tcaagcaagt gggcaactac ggcgaaagct tcgagcgcaa
      901 cgtcggtacc ggcagcgagc tgaagatcga acgtggcctc aacgccctgt ggaacaaagg
      961 tggtctgcag tacgcaccgc cggtgcgctg accgtccgct gccagtcact cgtcctgagt
     1021 ggctggcatt gttctgattg atgtgaacct catgcccgct aatgcgggca tgagggttcc
     1081 aagagggctc ctatgcaaac cactgcaaat gtcccgcgcc ccggtggatc ggtttggacc
     1141 gaccccaagg cgcgtgcttg gctatttcaa attcttgccg ttatcgccgt tgtggcgctc
     1201 ggctggtttc tgtttgacaa cacccagacc aacctggaaa aacgtgggat tacgtccggg
     1261 ttttcctttc tcaataacag cgccggtttc ggtattgccc agcacctgat cgactacgac
     1321 gaaagcaact cctatgggcg tgttttcgtg gtgggcctgc tcaacacctt gttggtgtcg
     1381 gtgattggta ttgttctggc gacgctgttg ggttttttgc tgggtgtggc acgtctgtcg
     1441 ccgaactggt tgatcagcaa gctggccacg gtatacatcg agacgttccg taatatcccg
     1501 ccgttgctgc aaatcttctt ctggtacttc gctgtcatgc tctccctgcc aggaccgcgg
     1561 cagagcatgg gcgttggtga agcgttcttc ctcaacagcc gcggcctgta tatgccagcc
     1621 ccaagcccga ccgacagctt tggcttgttt gccggtgcgg tgattgtggc gatcattggt
     1681 gtggtgctgc tggcgcgctg gtcgaaggcg cgctttgagg ccactggcca gctgtttccg
     1741 gtgtccctgg gggtaatacc gctgctgatc ggcctgccgg gtctggcgat tttgctgggc
     1801 ggcaacccgt tgcagtggag tgtgccggag ctgacgggct tcaacttccg tggcggctgg
     1861 gtgctgattc ctgagctgat ggcgttgacc ttggcgttga ccatctacac cgcagccttt
     1921 atcgccgaga acgtgcgttc cgggatcatg gcggtaaacc acgggcagac cgaagcggct
     1981 cgctctctgg gcctaccagc gggcaagacc ctgcgcttgg tgattattcc gcaggcgctg
     2041 cgggtgatca ttccgccgtt gaccagccaa tacctcaact tggcgaaaaa ctcctcactg
     2101 gcggccggta tcggttatcc ggacatggtc tcgctgttcg ccggcacggt actcaaccag
     2161 accgggcaag cgatcgaggt gatcgccatc accatgagtg tgtacctggc aatcagtatc
     2221 agcatttcca tgctgatgaa ctggtacaac aagcgcattg cgctgatcga gcggtgagga
     2281 gcagccatgc aaacgcatac gttcaaacct gatctaccgc cacccgcgct gagcgttggt
     2341 gtggtgggtt ggctgcgtgc caacctgttc tccagctggt tcaacaccct gctgacattg
     2401 tttgcagcct atttggtctg gctgatgctg ccaccgctga ttcagtgggc gtttattgat
     2461 gcggactgga ccggcaccac ccgtgccgac tgcaccagtg gcggcgcgtg ctgggtgttt
     2521 gtgcagcagc gtttcggcca gtttatgtac ggtttctacc cgagtgagct gcgctggcgc
     2581 gttgacctga ccctgtggtt agcggtcatc ggtgctgcac cgctgttcgt gccgcaaatg
     2641 ccgcgcaagg ccatgtatgg cctggctttc ctggtggttt atccgctgct ggccttctgg
     2701 ttgctgcacg gtggtttctt cggtctgtcg actgtgtcca ccagccagtg gggcggtctg
     2761 atgctcacgt tggtaattgc cgctgtgggt attgcaggcg ctttgccgct gggcattctg
     2821 ctggcgctgg gacggcgttc ggacatgccg gcgatccgcg tgatctgcgt gacctttatc
     2881 gagttctggc gcggtgtgcc gttgatcacc gtgctgttta tgtcctcggt catgttgccg
     2941 ctgttcctac ccgagggcct gagcttcgac aaactgatgc gtgcgttgat cggggtgatc
     3001 ctgttccagt cggcctatat cgccgaagtg gtgcgcggtg gtctgcaggc aattcccaaa
     3061 ggtcagtacg aggccgctgc ggccatgggc ctgggctatt ggcggatgat gggtctggtg
     3121 attctgccgc aagccctgaa gctggttatt cccggcatcg tcaacacctt tattgcgctg
     3181 ttcaaggaca ccagcctggt gatcatcatc ggcctgttcg acctgctcaa cagcatcaag
     3241 caagccacca ccgatccggc ttggctgggc atggccactg aaggctatgt gttcgccgcg
     3301 ctgattttct ggattttctg ttttggtatg tcccgctact cccagcatct ggagcgcaag
     3361 ctggacaccg gccacaagcg ttaggagtga gttatgagtg aagccaacaa gcaacccagc
     3421 gccgagccga tgatcctgct gcagggtgtg aacaagtggt acggccagtt ccatgtgctc
     3481 aaggacatca acctgagcgt gcagccgggc gagcgtatcg tgttgtgcgg gccttcgggc
     3541 tcgggcaaat cgaccaccat ccgctgcatc aatcggttgg aagaacacca gcaaggtcgc
     3601 atcgtggttg atggcaccga gctgacccat gacctcaagc acatcgaagc ggtgcgccgc
     3661 gaagtcggca tggtgttcca gcacttcaac ctgttcccgc acctgaccgt gctgcagaac
     3721 tgcaccttgg cgcctatgtg ggtgcgcaag atgcccaagc gtcaggcgga agagattgcc
     3781 atgcactttc ttgagcgcgt gcgcattccg gagcaggccc acaagtaccc agggcaactt
     3841 tctggtggcc agcaacagcg tgtggcgatt gcccgtgcgc tgtgcatgaa gccgaagatc
     3901 atgctgttcg acgagccgac ctcggccctc gatccggaaa tggtcaagga agttttggat
     3961 accatgatcg gcctggccga agacggtatg accatgctct gcgtaaccca cgaaatgggc
     4021 tttgcccgca ccgtggcgaa ccgggtgatc tttatggaca agggcgagat cgttgaacag
     4081 aacgaaccca atgccttctt caccaacccg cagaacgaac gcaccaagct gttcctcagc
     4141 cagatcattc actgagtttc agccgcaaca aaaaagggag ccttcgggct ccctttttta
     4201 tgcactcagc aacgctgtgt ggctatttgt tctcaggcca cagacgcaat ggcttacctt
     4261 cggcgggcca cagctgcagt tgctcggtgg cggagaagtc ccagcgttct accttgctca
     4321 ggctgtcgag aaagcgtgct tcctgctcca tcagcgccgg tgcgcacatc ttgcgggtgc
     4381 tgccggcggc gctgaagctg agcttgtcac cctccaggct gtaaccggca aaccagtggt
     4441 tgcagccggc gttgccatag gcgcggccgt cgtcaccgag ggtgatggtc aggtgactgc
     4501 ggtcgatcag tggacgttcg ccgatccatt ccacctggta gctgtgctcg gtttccagtt
     4561 gcggcgcgga tgtggcgcag cccacaaggc ccgtagccag caggctgata gtcagtacgc
     4621 gtttcatata gcctccttgt taatgcattt cgggcacagg tgcttggcgt ttttcgactg
     4681 ccagcccagc tccttgattc gtgcttcggc ggccggcgcc tgggcctttt tgccgagtgc
     4741 ggcgtcgacg gcgaactcga agtccagctg agcattgcag ctgtcgcagt tggcctgcca
     4801 attgaatatc gccagttcct tgaacgccgg accggtggcc acggcaaccc actggccgcg
     4861 cgggttgatt aggtggcgaa ccttgtcggc ctgcaggcgc atggacagct cacggctgcc
     4921 cttgagggtg accagtaggg tgtcgccgct ctggatcgag ccgccattgc cggtcacctg
     4981 gtaacggccc ggtgccaatg cgcggcattc gattagggtg tgctcggggt tgagcaggtt
     5041 gtagcgaaag tcgtgttcag ccatgaatcc tccaaatagg cgcaaatgct atcacgcggc
     5101 aggaagcagc tgattgatcg acgcatttgc cagccaggcg gcgatattgt cgcgggtggt
     5161 gccggcaatc gctgccagag cttcgtgggt gagaaagccc tggtgcgcgg taatcagcac
     5221 attggggaag ctcagcaggc gcgccagcac atcatcctgc agcggttgtt ctgagcaatc
     5281 ttcgaagaac agctcggctt cctcctcata aacatccagg cccagatagc ccagttgccc
     5341 gctttttagt gcgccgatca gcgccggggt atcgatcaag gcgccgcgtc cggtattgat
     5401 cagcattgcg ccgcgctgca tctgctgcaa acgcgtggtg ttgatcagat gctgggtggc
     5461 tgcgttgagc gggcaatgca ggctgatgat gttgctgctt gcgagcagtt gatccagcgt
     5521 cacctggcgc gcgccggcag gtagctcggc gatgggcgtt ggatcatgga tcagcagttg
     5581 gcagccgaaa ccggtcatga tccgcgcaaa ggtcaggccg atttgccctg caccgataat
     5641 gccgatctgt ttgccatgca ggtcgaaacc gatcagcccg tgcagggaga agtcgccctc
     5701 gcgggtgcgg ttgaacgcac ggtgagtgcg ccggttgagg gtcatgatca gcgccagggc
     5761 atgttcggcg acggcatgcg gcgagtagga gggcactcgt accactgtca ggccgagggc
     5821 gtgcgcggcg tgcaggtcga catggttgta gccggctgag cgcagggcta tcagccgtgt
     5881 accgccggcg gccagttgct gcagtacttg ggcgctgagg tcgtcattga taaaggcgca
     5941 aaccacttcg taaccggcgg cgaggccggc gctgtcctgg cgcaggtgtg cttcctgaaa
     6001 attcagttgc cagtcgtccg ggaaacccgc tgcactaaag ctctgctggt cgtaaggctt
     6061 gctgctgaac aggatgatgc gcatggggtt ccttggcgtg tggcttcgca gtgtctgaat
     6121 caggcgctga cctgggcgtg ctgggccagg cggtcgatgg cggcatccag ctcatccagg
     6181 gcttgttggg cggcggggtc gttctgtttc agcagagttt cgctgcgctg gcaggcggcg
     6241 cgcagctgcg gcacgccgca gtagcgggta gcgccgtgca ggcggtgtac gcgttcgatc
     6301 aggctgttgc gttcgccact gtcgcgcgcc tggcgaatgg cctgacggtc gccgggcagg
     6361 ccggccagca gcatgctcag catatccgcg gccagatccg ccttgccggc agccaggcgc
     6421 aggccttcgt cggcatccag tacgctgagg ttgttatgcg tggcgctgat gccggtgtgt
     6481 tcgtcgctct ggttgcgcag ggccaggccg gtccacttga gtaccacctg ggccagctga
     6541 cgctcgctga tcggcttggt caggtagtcg tccaagccgc tttgcagcag ggcgcgtttc
     6601 tcgttggcga gggcatgggc ggtcagcgcg acaatcggca ccgggccctg gttgcgctcg
     6661 ttttccagtt ggcgaattgc ctcggtggcc tggcggccgt ccatgccggg catctgtacg
     6721 tccataaaga tcaggtcgaa cgtttgctcg gcggctttct ccacggcctc atagccgctg
     6781 ttggcgatgc tcacctgcgc gcccatgtcg tccagcaggg tttgcaccag cagcaggttg
     6841 gccgggttgt cgtcgacgca gagaatcagc ggcgggcggc tcggggcgct ttgcttgttt
     6901 tctgcacggt tctgccgtgg gctgaccagc tcggccaggc cgcgctgtag cttgcgtgta
     6961 catgccggct tggcctgcag ttggctgtaa gcatccggca ataggctgtg gtatagcgat
     7021 tgttcggtgg tggggcacag cagcagggtc ttgcaggcca ggcgttcaag ctcccagatg
     7081 cgctgatcga ggcgctccgg cggcaaggtc tgggtgctga cgccgagcac cgccagctca
     7141 attggctggg cgctatgtcg cgcctcggcc acggcgctga gcaggctgtc gaggttgtcg
     7201 aagccgctga cctgcaggcc gcagtcttcc agttgatgct gcaaggcctg acgcgccagg
     7261 tcgtggtgtt caagggcggc cgcgcggcgg ccgagcagcg gtggccgcgg gaggtcttca
     7321 gcatcatcgc gggccttggg caggctcagg gtgatccaga attccgagcc ttcttcgggc
     7381 gtgctgtcga cgccgatttc gccgcccatc tgttcgatca gacgtttgga aatcaccagg
     7441 cccaggccgg taccgccggc ctggcggctg agcgaattgt cggcctggct aaaggcctgg
     7501 aacaactcgc gcaagtcttc atcagacaga ccgatgccgg tgtcctgcac gctgatgcgc
     7561 agctgcgcgc ggtctgcgct ttcgtcttcg agcatggcgc gaataacgat ggtgccggca
     7621 cgagtgaact tgatcgcgtt gcttaccagg ttggtcagca cctgcttgag ccgcagcggg
     7681 tcgccgatca gcgacagcgg cgtgtcgcgg tagaccaggc tgagcagctc cagctgtttg
     7741 gcgtgggcag cgggggcaag gatggtcagg gtgtcctgca gcaggtcgcg caggttgaac
     7801 gggatatttt ccagcaccag cttgccggct tcgatcttcg agaagtcgag aatctcgttg
     7861 atgatcgcca gcaggctgtc ggcggatttt tcgatggtgc ccaggtaatc ctgctggcgc
     7921 ggggtcagct cgcttttctg cagcaggttg gtgaagccga ggatgccgtt gagcggggtg
     7981 cggatttcgt ggctcatatt ggcgaggaat tcggacttga tccggctggc ctccagggcc
     8041 tctttgcgcg ccagatcgag ctcgatgttc tggatttcga tggtttccag gttctgccgc
     8101 acgtcttcgg tggcctggtc gacgctgtgt tgcagttctt cctgggcgtt ttgcagtgac
     8161 tcggccatgc gattgatgcc cgaggccaac tcgtccagct catggctgcc gagtggcggc
     8221 aggcgggttt ccaggtggcc gtccttgagc tgggcgacgc tctgtttgat ctgccgcagc
     8281 gggtcgttga tcgtgcggct catgcgcacg gcgagcaggg cggtgaccat cagtccggtg
     8341 gcgatcagca gcagactggc cagcagactg cggtagccgc tgagcagggt gctgtggtgc
     8401 gacagctcca gctcgaccca accgagcagg cgcgtgcctg gttcttcggt ggccttgcca
     8461 gtaaggctca ggtgctgttg atacaccggc aatagaaaac gcgtggcgtt ctggttgctg
     8521 ctctgcgcgg gttgtggctc acctgtcgtc ggcgaggggt tgagcatact tggccctgcg
     8581 tgggccaggg tgttgcgttc ggcggcgagg aagcttacgg cgcgcacatc cggctgatcg
     8641 agtgcctggc tggcaatgcg ctgtagcacc tcgctgtcac ccgaatgcaa cgccggcgcg
     8701 gccagtggcg cgagctgctc gatgatcatc tgcccacgct gcaacagttg cccctgcaaa
     8761 ccagacaact gcacccaggt gaagtagccg cccaacacca gcgccaacag gctggtgggc
     8821 aacagggtga gcatgagcac acggcctttg atgcctaaat cctttaacac gcgctgcctc
     8881 cttgggtgat gttttggcag tgtagcgatt cagccggcga gaatctgtag gtgagtgcgg
     8941 gagtgttagc aggtagaatt attcgcattt acctcggtgg ttctcccgtg ctcacttgta
     9001 accttccccc agcaaggatt cttgcggtcg aagatgaccc gctgctggcc agccacttgc
     9061 accagtacct gcaggacagc ggttactgcg tcaccctcag cggtgatggc agcgaagggc
     9121 tgcacctggc cgccgatggc gactttgatc tggtgctgct ggatatcctg ttgcctggca
     9181 tcagcggtct gcaggctctg cagagcctgc gtgagcagcg tggcgtgccg gtgattctga
     9241 tgtcggcctt gggtggcgag caggatcgaa ttgtcggttt cacccagggt gccgatgact
     9301 atctgcccaa gcccttcagc atggacgagc tgaaggtgcg tatcgaggcg gtgctgcgtc
     9361 gggtggctta tgagcggcgt gatctggctg tgttgtcggc gcagagaggc gggctgtact
     9421 ttgatgagca gcgtatggat atcagcctgg ctggccgctg ggccgggctg acgcctgcgg
     9481 aatatcgcgt attggagttg ctgtcgcgca acctggacga ggtgttgagc aagccgttcc
     9541 tctatcagca ggctttgcat cgtgcttatg cgctgcatga ccgcagcctg gatatgcacg
     9601 ttagtcatat ccgccgcaaa ttgcaggtgt taggtgacag cggcctgcgc ctggaaaccg
     9661 tgtggggcaa gggttacctg ctcactgggc aggggctcta agatgtcaat tcctggcctg
     9721 cattcattgt tctggaaact ggcggcattg ctggtcacct tcaccctgct gctgatttct
     9781 cttagctggt tctggagtca gcgggtcgag ttgcatacct cttacctgtc ccaacctgcg
     9841 aggcaacaac tgcaggcgta tgccgatgcc gccgagcagg cctggctgga gggtggagac
     9901 gccggtgttg ataagtttct gcaggatttg catgagcagg aagcgagtga tgcgctgctg
     9961 ctggacgctg agttgcgacc attgggtagc caggtgctga cgccgaagca gcgtgagcgc
    10021 ctgaccttta tgcgccagct ggactggccg atgagccgcc gctcggccga gttgccttat
    10081 ataaacatcc cgtttgccaa cggcgagggg catctggtta ttcaactgcc cgaacggttt
    10141 cgtccgtggg agtaccgcca accgctgtta tgggtgaccc gcgcattgct gccgggaatg
    10201 ctggcgttgc tgttctgtat tggcctttac cgggtcttga ttgctccctt ggtacgtttg
    10261 cgcgagcagg ccaacgcatt gcatagcgac gacttgtcgg cacgtgctgg agcagtggcg
    10321 cggcgccatg atgagttggg tgagctgggc cgcgccttcg accatatggc cgagcgcctg
    10381 cagggcacgg tggagttgca gcgccagttg ctgcgcgatc tgtctcacga gctgcgcaca
    10441 ccgctcagcc gtctgcgggt cgctgccgag ggcggcctgc aaggccaaaa cctgacgcag
    10501 cgcgtgatgc atgaagtgca gctgatgcag caactggtcg gcgatacgct ggagctggcc
    10561 tggctggata ccgagcgccc gcgactgacc cgtgagcagg tcgatctggc ggctctctgg
    10621 gacatgctgc gcgagcgcgc ctgttttgaa agtggctgga cccctgagcg tctggtgtat
    10681 gccgtcgagc cgggctgcca tctgtcctgc aacctcaatg ccttggcgcg ggcgctggaa
    10741 aatattctgc gcaacgccat ccgctattcg cccgaagccg ggctgattcg tctctgcggc
    10801 aaagcgcagg cgggtgaatg gcacttgtgg ctggaagatc aaggccctgg cgtgccagag
    10861 cagcagttag cgcagatatt tcttcccttc acccgcctca acggcgcccg gccaggtgac
    10921 ggcggctttg gcctggggct gagcatcgca agcagttcga ttaacgggca gggcgggcgt
    10981 atttatgctg aaaacgcccg tcccggttta cgcattcacc tacatctgcc ctcggcctga
    11041 ggctacgcgc gtgactgtcg ttatggctgt cgccggtttg ccgttatcat ggcggccttc
    11101 tttgcttctg gatcatttcg ttcgccatga gcccgcatta cccaaccatc gccgattgca
    11161 tcggcaacac cccgctggtg cgtttgcagc gcctgccggg taacaccagc aacaccttgc
    11221 tgttgaagct ggaaggcaac aacccggccg gttcggtaaa ggaccggccg gcgttgtcga
    11281 tgatcactcg cgctgagctg cgcggcgaca tccagcccgg tgatgtgttg atcgaggcca
    11341 ccagcggcaa caccggcatc gccctggcca tggcggcggc gatcaagggt taccggatga
    11401 tcctgatcat gccggacaac tccaccgctg agcgcaaggc ggcgatgacc gcctatggcg
    11461 cgcagctaat tctggtcggc ggcatggaag aggcgcgcga tctggccctg cagatgcagg
    11521 ccgacggcaa gggcaaggtg cttgatcagt tcgccaacgg cgacaatccg attgcccact
    11581 acaccagcac cggcccggaa atctggcagc agacgggcgg gcagatcacc catttcatca
    11641 gctcgatggg caccacgggg accatcatgg gcgtgtcgcg cttcctgaaa gagcagaagc
    11701 ccgacgtgca aatcgtcggt ctgcagccga tggaaggcgc ggcgattcct ggtattcgcc
    11761 gttggccaca ggagtacctg ccgaagattt atcaggccga acgggttgat cgcatcattg
    11821 atatgagcca gcaggaagcc gaagaggtga tgcgccgatt ggcgcgtgaa gaaggcatct
    11881 tctgcggagt gtcgtccggt ggttcggtgg cggcgatgct gcgcctgtct caggaagtcg
    11941 agaatgcggt gatggtggcg atcatctgcg accgtggcga ccgttacctg tccagcggcg
    12001 tttacgacgc tccgagcact gatggcaaaa gcaactgatg gccaagaaag acggcggtct
    12061 gcgctttcaa cccagcggtg gttcccgggc aacccaggtg ccggtgggca agaagcagaa
    12121 gctcaccatt gagcgcctgg ccaacgatgg ccgtggcatc gctttcagtg aagggcgtac
    12181 ctggtttgtc agcggcgcac tggccagcga gcagattgaa gcgcgagtgc tgggtgccca
    12241 cggcaaggtg gtcgaggcgc gggttgagcg gattatcact gctagtgagg aacggcgtaa
    12301 cgaggcctgc gcacatgcca aagtctgtgg cggttgcagc ctgcaacata tgccccatgc
    12361 cgatcagctt gccctgaaac agcgcatgct cgccgagcag ctcagccgtc tggccaacct
    12421 tgaaccgcag gagtgggccg caccgctggt cggcccggag ctgggctatc gtcgccgcgc
    12481 gcgtatcgcc gtgcgctggg acaacaaggc caagcgtctg gacgtcggtt ttcgcgcggc
    12541 ggcgagccag gacatcatcg ccatcagcga ttgcccggtg ctggtacaac ccttgcagcc
    12601 gatcctgcgc acgttaccgc tggtgttgcg cgagttggat aaacctcagg ccattggtca
    12661 tgtcgagttg ttcagcggca gcagcagtgc tgtgctggta aggcataccg ctgcactgag
    12721 cgaagcggac ctggctcgct tgcaggcgtt ctgcacggcg cagaatgcgc agctgtggtt
    12781 gcacggcacg gccgagccgc agccgtttga gccggcgcag cagctgagtt atcgcctgga
    12841 tgcgtttgat ctcgacctgg cttaccggcc tggcgatttt gtccaggtca accagccggt
    12901 gaatgacgcc atggttgccc aggccctgga atggctggcg ccgcaggccg atgagcgggt
    12961 gctggatctg ttctgcggtc tgggcaattt cgccttgccc ctggcccaac gggtgagtga
    13021 gctggtggcg gtggaaggcg tgcagaccat ggttgagcgc gccgctgaga atgccgccag
    13081 caatggccta agcaatgcgc acttttacca ggccgacctg tccaacccgc tggccgaggc
    13141 tggctgggcc caaggcggtt ttgccgccgt gctgctggat ccgccgcgcg atggcgcgct
    13201 gcaggcggtc aagcaaatga cagtgctggg cgtcaaacgg gtgctgtatg tgtcgtgcaa
    13261 cccgacgacc ctggctcgcg atagcgccga gttgctggcg caaggttatc ggctggaacg
    13321 cgccggtatc ctcgatatgt ttccgcaaac tgcgcacgtc gaagcgatgg cgctgtttgt
    13381 caggcagtag atagaagcgc cgtatcccgg cgctgcaggg gcgcgcaggg atcgcgcctt
    13441 aagtggttaa accgaccg
//
LOCUS       DASRUM010000209        50656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_941627, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000209 DASRUM010000000
VERSION     DASRUM010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 50656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50656
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941627"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VFF22_13720"
     CDS             <1..985
                     /locus_tag="VFF22_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZX17904.1"
                     /translation="ALATRVAQSDSTVLITGESGTGKEVLARFIHQQSPRGSKPFIAI
                     NCAAIPDNMLEATLFGHEKGAFTGAIASAPGKFELAEGGTILLDEISEMPLGLQAKLL
                     RVLQEREVERVGARKPIILDIRVLATSNRDLAAEVAAGRFREDLYYRLSVFPLAWRPL
                     RERPADIVPLAERLLAKYVKKMNHAPIRLSPQAQMSLMSHSWPGNVRELDNAIQRALI
                     LQQGGLIQPQDLCLLAPIGSAVTPPAQPLLAVVVNSAPAQQDVSPAEAGALGDDLRRH
                     EFQMIIDTLRSERGRRKEAAERLGISPRTLRYKLAQMRDAGMDVEGYLFAS"
     gene            complement(1171..3120)
                     /locus_tag="VFF22_13725"
     CDS             complement(1171..3120)
                     /locus_tag="VFF22_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003447941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZX17905.1"
                     /translation="MSLLKQLFLAICLFLVVAFTGSFIAGVESSREQLLSQLRSHAQD
                     AATALGLSMTPHVDDPAMIELMVSSIFDSGYFTSIRVIRIPNNEVIVERRSDDASAET
                     VPVWFSELVNLQPQGGDALIMRGWEQAARVEVLSHPQFALAKLWDSAIGSLIWLLLCG
                     LVSAVLGGWLLRTQLRPLDNMVQQAQAITRREFLTLPKVPRTPELKRVVTAMNQMVEK
                     LKTLFAEEAARSEKLREEAYQDSITGLANRRLFDIRLAHQLVVSEQNAEGYLLLLRVN
                     DLGGLNQRLGGQQTDALIAAIGELLKRLLNQTNRSDWLASRSRGGEFSLLAPGIGSEE
                     ADLLAGELHSGLESLRQTGASDCTPVAHMGIAAFRPGEEPSQVIGRADQALAQAQNDP
                     SRDWVRLDDYNAQPTQGLHEWRTWIDDALNKRKLQLYFQPVTQCADRNRLLHHKVLAR
                     LLDPKGEAIAAGRFLPWIERLGWTARFDLAMLEHSLAHLSQHAAPLALSLSAATLRDT
                     ETLKRMFEILRQHPQQASLLTLEVDERHLPPAAELEALSQGIRDTGFSLGLQHFGGRF
                     SLIGNLTHLGLAYLKLDGTYIRAIDQESDKRMFIEAVFRATNSIDLPLIAEMVETEGE
                     LAVLKELGLYGAMGRLLGAPMPWQD"
     gene            complement(3130..3822)
                     /locus_tag="VFF22_13730"
     CDS             complement(3130..3822)
                     /locus_tag="VFF22_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HZX17906.1"
                     /translation="MPPRQLTHGWFKRLNRALLGGCILLIGLASALANWDFGVILQNA
                     ESRYGNLGAAKQRIQAWEAQIKASDSSSERDQLTEVNRFFNRQIRFTDDIRLWKQNDY
                     WATPIEMLVKGEGDCEDYSIAKYFTLRRLGIPSDKLRITYVKALNYNQAHMVLTYYAS
                     PGAEPLVLDNLINEIKPASQRKDLLPVYAFNAEGLYLPGSTSRKSDSKKLSRWQDILK
                     KMRTEGFAIGEG"
     gene            complement(3915..4328)
                     /locus_tag="VFF22_13735"
     CDS             complement(3915..4328)
                     /locus_tag="VFF22_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HZX17907.1"
                     /translation="MAREIQVPPVQEQRNPIIRYVLLAIGWLSVALGVIGIFLPVLPT
                     TPFLLLAAACFMRSSKRFYLWLVNHRRLGPWIVDYLEGQGIPLKGKVYAIGVMWLSIG
                     LSCYLVPLFWARAFMLTSVVLVSLYILSQKTLIKP"
     gene            complement(4338..4925)
                     /locus_tag="VFF22_13740"
     CDS             complement(4338..4925)
                     /locus_tag="VFF22_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HZX17908.1"
                     /translation="MSFAEQLSRLQAFLDADELHDEALDYIAAHGYLTALSICPQPVP
                     EREWIDALFSEPPHYRSDAEREEIEATLIQLQSHIARQLASDDEPDVPCDLDLGEEPD
                     DSDLRGWCIGFMEGVFLRESVWFDDAEDEVSELLLPIMVGSGLFDEQPEFAEIARDRD
                     LVDSMVEQIPELLTALFLLCNAPEEKPALLKPRHH"
     gene            5088..7217
                     /gene="recQ"
                     /locus_tag="VFF22_13745"
     CDS             5088..7217
                     /gene="recQ"
                     /locus_tag="VFF22_13745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HZX17909.1"
                     /translation="MLEHAQRILKDVFGYDSFRGRQGAIIERVASGGDALVLMPTGGG
                     KSLCFQVPALLREGLAVVVSPLIALMDDQVATLDELGVAAAALNSTLNADEQREIAER
                     IKRGEIKMLYLAPERLVQPRMLSFLQNLDIALFAIDEAHCVSQWGHDFRPEYLQLGQL
                     AELFPNVPRIALTATADMRTREEIVQRLHLQNAERFLSSFDRPNIFYRIVPKEQPRKQ
                     LLNFLAARKGDAGIVYCLSRKKVEEVASFLSEQGFPALPYHAGLANELRAYHQKRFLN
                     EEGLIMVATIAFGMGIDKPNVRFVAHLDLPKSLEAYYQETGRAGRDGLPADAWMAYGL
                     QDVIFLKQMLNNSEGDERHKRVEQHKLDAMLALCEETRCRRQALLAYFDEVMPQPCGH
                     CDNCIDGVQTWDATEPARQALSAIYRTGQRYGVGHLVDVLLGRDNEKIRGVGHQHLAV
                     FGIGKALAEGEWRSLFRQLVARGLADVDLEGFGGLRLADSCRPLLKGEVTLQLRRDLK
                     PQQSAKASSSAASQLVRGEERGQWEALRALRRKLAEEHGVPPYVIFPDATLLEMLRSK
                     PSTLAEMARVSGVGARKLERYGEAFLAVLGADDEAPRAVVDLRHELVSLARAGMTPAQ
                     IASQLSCSEKNVYGLLAEAIGRQQLSLEQALDVPEALLGEIQDAFLDGEGELPPVAAI
                     SEQFVGRVELGILHCVRAALQAEFEAE"
     gene            7378..8466
                     /locus_tag="VFF22_13750"
     CDS             7378..8466
                     /locus_tag="VFF22_13750"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZX17910.1"
                     /translation="MLRFSRPLEQEYRDYVRDDTFDLKRIALGVGILVWLAFAAFDFV
                     LIKAPEVWWMFAIRLAVLLLLCLFGGLLIQRKHVHLLQPLSLVSLLALGAAASFVVGI
                     AHRVDPSYPYEGLLLVCMAAYFLAGLRLIEALCCSLLVLLVYVCVELLVAGLPLPRLL
                     NNTLFLLLGNLMGAVGCYLLEFKSREHFLISRLMRVLADHDSLTGLHNRRSFNRQFER
                     LWRQAQREHRSLALLLCDVDHFKAYNDHYGHQAGDNVLQRISSLLQDAARRPLDMAVR
                     LGGEEFAVLLYDIGEVEARRHAEALRETLQTLNIEHAGSATASMLTMSIGISCIQPAV
                     GGALAQLYEHADRALYEAKAFGRNQVVA"
     gene            8582..9598
                     /locus_tag="VFF22_13755"
     CDS             8582..9598
                     /locus_tag="VFF22_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HZX17911.1"
                     /translation="MDRLIQANGQPHYGIFPAAPGLINYRDFDFRSPMGRKLGALSKW
                     RRFHQFQYFGLISDELIGGCALANLSLLGVGFVYLFHPASGRMIERQFKLPLGFGSQF
                     SQTPDQGVCEVRSGGNLLRLENIAASKEKRLLVELDDGTRIDACFSEAEPAFQPMCIN
                     TPTAVNGWVYAQKVAGVRCTGQVRSALGEFDLAQIGAFAHHDWSAGYMRPETHWNWAC
                     LSGLAGEQRVGLNLSCGVNESSFTENCFWLDGELLKVDTVRFAYDRDHSLEPWLINSF
                     DGQVKLRFEGRGLHKERLNVGLLASNFKQIFGQFSGVLRPTGRPEVRIDNLWGFVEDQ
                     YVKW"
     gene            9681..10115
                     /locus_tag="VFF22_13760"
     CDS             9681..10115
                     /locus_tag="VFF22_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZX17912.1"
                     /translation="MSHTDQHRFAMQVAQLSRAWRAELDRRLVGLGLSQARWLVLLHL
                     GRFAELPTQRELAQSVGVEGPTLARLLDSLEAQGLVSRQAVPEDRRAKKIALSPQARP
                     LIEKIEAISAQLRQEVFAGIDEDELRRCQKVHAQVLSNLEKR"
     gene            10115..11518
                     /locus_tag="VFF22_13765"
     CDS             10115..11518
                     /locus_tag="VFF22_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZX17913.1"
                     /translation="MRDDLQSLQQRFGPRYPQWLLALLMVGSMAMVLASTSINVALPA
                     IMADFAIGRPLAQWLSTGFLAAMTAGLLLAAWAQARFGARRTAQFGLLLFVLSSLLAL
                     VAHAAWQLIALRIVQGLCAGIVQPLAMVLIFRVFADGGRGMALGIYGLGVMLAPSLGP
                     GIGGYLVDHFGWPSIFWMPLPMCLLAMLAGQWLLPSERERSTPRLDIQAFAWLCVAIF
                     AALGFLAEAQRFAWSAPRVWLPGCLALLAGAAFILRSRSAAQPLLPLALWRHAGFRSA
                     SWVALTLGLGLYGSTYLIPLYLQTVEGFSAGRSGLLLLPAGILMGMASFLGGWLSDRL
                     SAAVLLSAGLLIFALSAAGLAWVEQGASFIQLCFWACVGRIGLGILLPALSTGSLDIL
                     SPAELAQGAGAITFVRQLGGAFGVNLLTFFLEWRHSAEGGDHFAEMLAFKQSFWLVAG
                     VFAITVLAAWGVKSTRR"
     gene            11790..13844
                     /locus_tag="VFF22_13770"
     CDS             11790..13844
                     /locus_tag="VFF22_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HZX17914.1"
                     /translation="MAKVRQLLIGLASSSALYSGLVPALGLGEITLHSALSQPLDAEI
                     ELFDVGDLSDADMRVRLAPAEVFSRSGVDRLYFFNDLRFTPLLRGGKSVIRVVSTQPV
                     REPYLNFIVEVVRPNGQLYREYTVLLDPPSSAAYRSLAAPAASAAPAAVASAPRATTV
                     ERARSAVLPAAQQGNRYSVVSGDSLWKIATRLAVDAGQVAPQALMNDIHALNPQAFIN
                     GDINRLRAGAQLLLPDSVADSSPSVIEPAAPITTPAVAPPEIAPTVSAELPVDPPAEL
                     LIQEQARVEQELASQISETLQLQQSLAQLGLQVQQLQEQMGARDQQIAGLQAELAARR
                     ASAEAIAAPVSAPVAENPSATAESGNNWLAIIAGLLTLLLAGVAGLFWRGKKPVEPAP
                     VPAQPARAASMPKAQSMAPALPVAPAAVALNVRDGPVWPAAGEDALQSANVYIAYGRL
                     SEAASTLRKAWEAQPQRSDIGFRLLEVLALLGDAQGFVAHEVLVRATGFTPARIDELK
                     ARHAELFSAPPSSLLDDVVLELDEVEAQPLVDAANDGQLNLDDMSLDADWDLVSPFAP
                     TAPKKVANKAESNAATKRKTSDIFELSGSRDARSPYVESMLVEETSHDGWGGDVLDVA
                     LLDDLDELAGNRDNLSKLNMALAYIEQGSLDSACQILNEVINEGDEEQKQEARQLLAK
                     IA"
     gene            13960..14241
                     /locus_tag="VFF22_13775"
     CDS             13960..14241
                     /locus_tag="VFF22_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelT/SelW/SelH family protein"
                     /protein_id="HZX17915.1"
                     /translation="MSSPKAEVVITYCTQCQWLLRAAWLAQELLSSFTDDLSRVCLEP
                     GTGGVFRITCDGVQIWERKADGGFPEAKVLKQRLRDQIDPARDLGHNDR"
     gene            complement(14264..15115)
                     /locus_tag="VFF22_13780"
     CDS             complement(14264..15115)
                     /locus_tag="VFF22_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZX17916.1"
                     /translation="MNQRNALWAIHLGALLFGLSGIFGKLANTTPQMISAGRAVFAVA
                     ALLLFAKLISNARLVRLGLRQTSLLALGGLLLGGHWWSFFESVHISGVAIATLGFASF
                     PAFTVLLEGLLFRERTRPIEYAMVALVCLGLVLVTPDFDLSSQATSGLLWAVLSGLLF
                     SLLSLLNRASTRGIDPVQAALYQNLTVLLVFIPLAWPTLPSVQAQDWLWMAMLGIFCT
                     GLAHSLFVASLRVLKARTTAVIFALEPIYGIAFAWLLFSEVPTLRMLAGGLLIVSAIF
                     LSARMAK"
     gene            complement(15112..15447)
                     /locus_tag="VFF22_13785"
     CDS             complement(15112..15447)
                     /locus_tag="VFF22_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HZX17917.1"
                     /translation="MHNIEWQDFEKVELRVGTIRAARTNEKAVKPAYVLEVDLGELGT
                     KTSSAQLTAHYSCEQLLDRQVLCVCNFAAKRIAGVRSEVLVTGVYDADNKVVLAGFDK
                     PLPNGARLA"
     gene            complement(15556..16305)
                     /locus_tag="VFF22_13790"
     CDS             complement(15556..16305)
                     /locus_tag="VFF22_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZX17918.1"
                     /translation="MSSLLSLRHYSHELLSHSHDHAQLVFGLSGLLEFEIAGHGSQVK
                     RQTLAVVPSDAQHACGSKSGSHCLVLDVPEDSWLQQQLGRHLDATRRLLEKPAAVSLN
                     PAQSQLVSWLAASPINDPVIAQQGAALLLASLANGEMPAHAASRLPLAALDAHIDQHA
                     AHPLQVADLARLAGLSAARFHARFLDETGQTPMDYVRSRRLQLARQLLQGSNLAIGEI
                     AARVGYSSQSAFSAALSRHLGVTPRQLRRTE"
     gene            16431..17249
                     /locus_tag="VFF22_13795"
     CDS             16431..17249
                     /locus_tag="VFF22_13795"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018929924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HZX17919.1"
                     /translation="MTSAQLAKPSRKQRVRTLWISDVHLGTRDCQAEHLAAFLKRYQT
                     DKIYLVGDIIDGWKLRSGVYWPQSHSNVIRRLLTMSKRGTEVIYVTGNHDEFLRRYSS
                     LMLGNIQLVDEAEHITADGRRLLVIHGDQFDVITRYHRWLAFLGDSAYEFTLTLNRWL
                     NYWRSRYGYGYWSLSAYLKHKVKTAVNFISDFEEAIVHECAKRGFNGVVCGHIHHAEI
                     RPMGDVEYMNCGDWVESCTALIEHLDGSIELYRLADDQARQLQAAPVVAAEPAL"
     gene            17246..18277
                     /locus_tag="VFF22_13800"
     CDS             17246..18277
                     /locus_tag="VFF22_13800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZX17920.1"
                     /translation="MRILIVSDAWSPQVNGVVTSLQALISELRGLGHQVKLLSPADFR
                     ALPCPSYPEIPLVWSLWRVGPAIRAFQPDCVHLATEGPLGWAARRWLVKRDLAFSSAI
                     HTRFPEYVHTRWPWIPIRWGYAFLRAFHRPSQAVLVTTERMRDEFAGWGFKRLVLWRK
                     GVDTQLFRPSLALPNLDQPVFLYVGRVAPEKNIEAFLALDLPGQKQVVGDGPQREALQ
                     TAYPQVRFLGYQHGQALAEAFQNASVLVFPSRTDTYGLVMLEALACGTPVAAFPVAGP
                     LDVLQAGVSGVMHEDLRAACLEALSLDRGRCAELAARQSWRASALEFLARQPLLDGEL
                     CLQAQVGAT"
     gene            complement(18270..19658)
                     /locus_tag="VFF22_13805"
     CDS             complement(18270..19658)
                     /locus_tag="VFF22_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZX17921.1"
                     /translation="MNVKSAPRISALPAPPLREYYSRVLAYIAIAASIAAGTYTQHFG
                     YDILWMVPYALLYPHLAHHLSLRFKRDHPQRTTLSLLFIDALHAGGVLALLGFSVVPS
                     LMSLLILLFSALVIGGVRHLALASLIALSGAVFCAALFQVKLNINTPPLVALVSIFFT
                     TLYICITAYFVHQQGLRLAQVRSEITREQEKAARLARNLAKYLSPQVWESIFTGKKSV
                     RLETQRKKLTVFFSDIKGFTELSEELEAEALTDLLNTYINEMSKICLKYGGTIDKFIG
                     DCVMVFFGDPSSKGAKKDAVNAVSMAIAMRKHMKVLRQQWRAQGITKPLEIRMGLNTG
                     YCTVGNFGADTRMDYTIIGRDVNLASRLESAAESGEILISHETYSLVKDVIMCRDKGQ
                     INVKGFTRPVQIYQVVDFRRDLGATSSYVEHELPGFSMYLDTNGIQNFDKEKVIQALS
                     QAADKLRDKVIM"
     gene            complement(19820..20137)
                     /locus_tag="VFF22_13810"
     CDS             complement(19820..20137)
                     /locus_tag="VFF22_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HZX17922.1"
                     /translation="MSTETHERYNSFAEFYPYYLQEHSNATCRRLHYVGSLLVLCVLG
                     YALITQQWLWLLAIPLIGYGFAWVGHFVFEKNRPATFDYPLYSLMGDWVMLKDAFTGR
                     IKF"
     gene            20294..21295
                     /locus_tag="VFF22_13815"
     CDS             20294..21295
                     /locus_tag="VFF22_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015372822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZX17923.1"
                     /translation="MSERTTSSSWASGIVAALELGGVDCRSLFSQLGLDYAALTDADA
                     RFAQDAMTRLWHLAVEASGNPAIGLNMAKILRPSSFNVVGYAVMSSRNLQEGFARLVR
                     YQRIIGEGADLSFHPTPEGYEMLLAIHGDRLPSARQSAEASLAHSLAFCRWLTGKPLR
                     PRLVSLMGPAPADLAPYEEVFQCPLKFNAEHYALLFERADLDAPLPSANEALAQLHDR
                     FAGDYLARFSGTRVTHQVRQVLCRLLPQGEPKRESVAQALLLSERTLQRRLQDEGTSY
                     QHLLDDTRRELAGQYLGQANLTLLEISYLLGFSDPSNFFRAFRRWFDTTPGEYRARM"
     gene            complement(21304..22758)
                     /locus_tag="VFF22_13820"
     CDS             complement(21304..22758)
                     /locus_tag="VFF22_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019828762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HZX17924.1"
                     /translation="MAWPTLRIIAFINGIFLLTLAISMAVPMLTLVLFTRPDELNAFL
                     WSSLITFIAGIAMIAQGRPKDVQLRPRDMYLLTVSSWILVGLFAALPFMFSQHLDVTD
                     AVFESMSGITATGATVLTGLDSMSPGILIWRSLLHWLGGIGFIAMAVAILPMLRIGGM
                     RLFQTESSDRSEKIMPRSHMVAKYMVAAYVGISLLGCLALWTAGMGVFDAINHTMSAI
                     ATGGFSTSDQSVGKWTEPAVHWVTIALMILGSVPFVLFASMLRGNYKALIRDQQVRGF
                     LGILLSTWLLLGTWYYLSTELHWLEAIRHVAFNTTSIMTTTGFALGDYTLWGGFAGML
                     FFYLGFIGGCSGSTAGGLKIFRFQVAYVLLRANLMQLIHPRAVIKQQYNRHHLDEDIV
                     RSILTFSFFFTITIAVLALGLTLCGLDWITALSGAASTVSGVGPGMGPIIGPAGNFSS
                     LPDTAKWLLTFGMLLGRLEILTVLVLMLPAFWRH"
     gene            complement(22835..24289)
                     /locus_tag="VFF22_13825"
     CDS             complement(22835..24289)
                     /locus_tag="VFF22_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HZX17925.1"
                     /translation="MSLPTLRIIAFILGIFLITLAVSMLIPMLTLLLYSRTDDLNAFL
                     WSSLVTAAAGIAMVIPGRPKEAQLRPRDMYLLTTGSWLVVCIFAALPMVLIHHISYTD
                     AFFETMSGVTTTGSTVLAGLDHASPGLLIWRSMLQWLGGIGFIGMAVAILPLLRVGGM
                     RLFQTESSDWGEKVMPRSHMAAKYILFIYLSLTLTGFLGFWLAGMTPFDALNHAMTSI
                     STGGYSTSDSSLANWSQPAVHWVAVVLMIFGGLPFTLYVATLRGNRKALFKDHQVRGF
                     LGFLLVTWLVFGTWLWLHSDNSWLDAFRIVAVNVTSVVTTTGYALGDYTTWGSFAVLL
                     FFYLTFVGGCSGSTSGGLKIFRFQVAYVLLKANLQQLVHPRAVIKQQYNNHNLDEEIV
                     RSLITFSFFFTITIGVIALGLTLLGLDWVTALTGAATAVCNVGPGLGPIIGPAGNFSS
                     LPDAAKWLLTIGMLLGRLEILTVLVLVTRAFWRH"
     gene            24444..24815
                     /locus_tag="VFF22_13830"
     CDS             24444..24815
                     /locus_tag="VFF22_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZX17926.1"
                     /translation="MSDSNPCLSCGACCAHFRVSFFWGECQSAGGVVPDDRVVLITPH
                     RVAMRGTEHKPVRCTALLGEVGQGVRCTLYEQRSSTCREFEASWVNGEHNPHCDAARG
                     AHGLPPIMPPVLPSISPERVA"
     gene            24890..25447
                     /locus_tag="VFF22_13835"
     CDS             24890..25447
                     /locus_tag="VFF22_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZX17927.1"
                     /translation="MDALDALLNRVSAPRLIAPAPDAAQRELLFRAALRAPDHGQLRP
                     WRFLTVEGEALTQLGALFASALPADASPEAVTKARAMPLRAPLLVVVIARVQASLKVP
                     AQEQVLAAGCAAHGILLAAHAQGIGAVWRTGDMAYNPQVAAGLGLSADEQVIAYLYLG
                     TPERELRAVPQVQVGDFVKAWPAVE"
     gene            complement(25422..26756)
                     /locus_tag="VFF22_13840"
     CDS             complement(25422..26756)
                     /locus_tag="VFF22_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007948562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZX17928.1"
                     /translation="MRSLFWRIFASFWLAIALVAGLSMLLGRMLNQDAWLLSQHPGMD
                     DLAEKWTQRYEKKGPSSAQALLEQRKRKFRIDVQVLDDSGQAVVKGTFPPRAAAFEAR
                     HSNEKRLPWRRLSVDYTSPHSGETYLFIYRIPNPEVAAWQRESLLWPLSALAIAMLVL
                     TLFSLWLTLSITRPLNRLRGAVHDLGQTAYQQNSLAKLAERRDELGVLAADFNRMGER
                     VQGLISSQRQLLRDVSHELRSPLARLRIALALTERADAAEREKYWPRLSQECDRLEAL
                     ISEILALARLDAEPGAAQPINVDELLHRLQEDAHLDAPEQQIKIQLESDLQLNGWLDM
                     LQRAMDNLLRNALRFNPVGQAIELTASKNAEQLCLSVRDHGPGVAPELLTRLGEPFYR
                     APGQTSSGHGLGLAIARRAAERHGGQLELSNHAQGGFIATLRLPLTPPQARP"
     gene            complement(26847..27266)
                     /locus_tag="VFF22_13845"
     CDS             complement(26847..27266)
                     /locus_tag="VFF22_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HZX17929.1"
                     /translation="MRKTLTAVLLALTLPTLAMAMPDGGPRHGGGHHGEHGSRMFKEL
                     DLSKEQHREIRKLMGEQMQGRHEITKRYLDKLPAAEQKAMQDELEAAKDKQHSAIRAL
                     LKPEQQKAFDEHQKKMQERRAERQAFKAWQAEQAKKN"
     gene            complement(27397..28074)
                     /locus_tag="VFF22_13850"
     CDS             complement(27397..28074)
                     /locus_tag="VFF22_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004884298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZX17930.1"
                     /translation="MSDLLLIDDDVELCELLTSWLTQEGFQVSACHDGHSARNALAKH
                     SPDAVVLDVMLPDGSGLELLKQLRSDHPELPVLMLSARGEPLDRILGLELGADDYLAK
                     PCDPRELTARLRAVLRRSLPATPSSPLDLGDLHYSQARGTVTLGEQEVLLTLSESRIL
                     EALLQHPGEPVDKQELAQLALGRKLTLYDRSLDMHVSNLRKKLGPHADGRQRILALRS
                     RGYYYSV"
     gene            complement(28078..28479)
                     /locus_tag="VFF22_13855"
     CDS             complement(28078..28479)
                     /locus_tag="VFF22_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor 2"
                     /protein_id="HZX17931.1"
                     /translation="MRPGPLFLLLTLCLPAVTQAEESPPQPLAEATSVQTQIDELEQR
                     LALSEEQREALSTELQNSSNERDTVQLQRLRQENQRLKLQLKKAQASAPQPLISEQQM
                     WFATGAGAGLLGVIVGALLRRGRRSRSEWLN"
     gene            complement(28533..28832)
                     /locus_tag="VFF22_13860"
     CDS             complement(28533..28832)
                     /locus_tag="VFF22_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZX17932.1"
                     /translation="MLYAIIATDVENSLENRLSARPAHLARLEQLKTEGRLILAGPHP
                     AVDSNDPGPAGFSGSLIVAEFESLQAAQQWAEADPYRAVGVYASVLVKPFKLVLP"
     gene            28984..29847
                     /locus_tag="VFF22_13865"
     CDS             28984..29847
                     /locus_tag="VFF22_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZX17933.1"
                     /translation="MDVDLHCHSTASDGALAPAVVVARAFERGVRLLALTDHDTLDGL
                     DEAGAAAQALGMQLVNGIELSCTWGGATIHVLGYAFNRDAPALQQAIAELHEGRWLRA
                     AEISKRLEAKGMPGALEGARAMQQELGDSGNAPARPHFADFLVRSGYVKDRAEAFRKW
                     LGSGKLGDVKQHWPELAQAVDTLRRANAWVSLAHPWQYDFTRSKRRKLIADFVGAGGH
                     SLEVVNGMQPAEQVGSLAILAREFGMFVTAGSDFHAPGQWSELGVYRPVPEDLPPLWA
                     RFQHVQQPTAI"
     gene            29861..30490
                     /locus_tag="VFF22_13870"
     CDS             29861..30490
                     /locus_tag="VFF22_13870"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZX17934.1"
                     /translation="MSQFFQIHPENPQARLIKQAVEIIRNGGVVIYPTDSSYAVGCHI
                     GDKNAVERIRRLRQLDDKHNFTLVCRDLSQLSTFAKVDTAAFRLLKSHTPGPYTFILN
                     ATREVPRMLLHAKRRTIGLRVPNHPIAMALLAELDEPLMSVTLILPGETLPMSDPYEM
                     RQILEHQVDLIIDGGFGGLEASTVVSLLDDEPEVFRVGCGDPSPFTNDI"
     gene            30535..31749
                     /locus_tag="VFF22_13875"
     CDS             30535..31749
                     /locus_tag="VFF22_13875"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZX17935.1"
                     /translation="MSLVDAERRVLSGMRPSGLLHLGHYQGVLKNWVKLQHTYECFFC
                     IVDLHALTTDYDDVGPLSQRVMDMAVDWLAAGVSPSSATLFIQSQVPEHAELHLLLSM
                     ICPLSWLERVPSYKEQQEQLHGKDLSTFGFLGYPLLQAADILLYRAGVVPVGSDQLAH
                     IEFARDVARRFNHLYGREPGFEEKAEAAIIKLGKKTATLYNNLRKAYQQQGDAEALET
                     ARALLKDQQSITLGDKERLYGYLEGGGKILLIEPQAMLGESPKLLGLDGGKMSKSNNN
                     AIFLRDSGAAIEEKIRRMPTDPARVHRDDPGSPANCPVWPLHQLYSSNETCDWAQQGC
                     RSAGIGCLECKAPLVEALQQELQPLQARAADYQDNPDLVRQILADGADRARSEARETL
                     SEVRLALGLNYR"
     gene            31923..32621
                     /locus_tag="VFF22_13880"
     CDS             31923..32621
                     /locus_tag="VFF22_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003175762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HZX17936.1"
                     /translation="MEVFLEAFEGPLDLLLYLIRKQNIDVLDIPVAEITRQYMGYVEL
                     MQSVRLELAAEYLVMAAMLAEIKSRMLLPRSSEVEAEEDDPRAELIRRLQEYERYKAA
                     AEGIDGLSRVGRDVTVPRLDAPEARARKLLPDVSLEEVLLSMAEVLRRADMFESHQVT
                     REALSTRERMSEVLERLKGGAFVPFVSLFSAEEGKLGVVVTFMAVLELIKESLVELVQ
                     NEAFGPIHVRARAE"
     gene            32624..33340
                     /gene="scpB"
                     /locus_tag="VFF22_13885"
                     /pseudo
     CDS             32624..33340
                     /gene="scpB"
                     /locus_tag="VFF22_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003204867.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
     gene            33673..34872
                     /locus_tag="VFF22_13890"
     CDS             33673..34872
                     /locus_tag="VFF22_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004396557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZX17937.1"
                     /translation="MSEEIEEYSPAGEKLQKVMARMGLASRREIEAWITAGRVKVNGA
                     VASLGVRVDLHDAIAVDGKVIRREEAAESVRRVIIYNKPEGEICTRDDPEGRPTVFDR
                     LPRPKDGRWINIGRLDINTTGLLMFTTDGELANRLMHPSFQMDREYAVRVRGEVDEEM
                     IERLKAGVMLEDGPAKFTDIKVAPGGVGFNHWYHCVVMEGRNREVRRLWESQGLVVSR
                     LKRVRFGPVFITSDLTMGRWREMSQREVDILSEEVGLKPVALPDMKEKARDKIDRLQR
                     KSAKPVGRAARPERTLRPAHGGGPAAAGDQGRSRPARGEGAERGERPVRNPRTPRVDQ
                     SKGTPVAERPSDVNKKRPAKPGAQRSGVELSERPSRKPAGAPVKRRSPPSREGQRPGF
                     GRGPKPE"
     gene            complement(34932..35858)
                     /locus_tag="VFF22_13895"
     CDS             complement(34932..35858)
                     /locus_tag="VFF22_13895"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008048989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZX17938.1"
                     /translation="MAPHNQSNTIDFDAAKLQRLGYAGRLQKSLKPVSLAQLRQQLSL
                     RLQTSLEAERILELFFTEVQRLVPLSALSYHLASCDLRLDLGERGNHSAGYRLNHDGE
                     YLGELTFRRNQRFSEDELGQLESLLASLLFPLRNALLYRSAVQSALRDPLTDTGNRIA
                     MQQTLKREVDIARRTLQPLSVLMVDIDHFKRINDNHGHLIGDQALKAVATELKDSLRN
                     VDMVFRFGGEEFMVLLSNTNREAASMVGERLRMAVLGIQYLVESRAIELSVSLGCATL
                     LPGESVESLLRRADNALYVSKRDGRNRLSMAG"
     gene            complement(36076..36816)
                     /locus_tag="VFF22_13900"
     CDS             complement(36076..36816)
                     /locus_tag="VFF22_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HZX17939.1"
                     /translation="MHDYSARPDLLKGRVILVTGAGRGIGATAAKTFAAHGATVLLLG
                     KTEENLSRVYDAIEAAGHPQAAVIPFNLETALPHQYDELAAMIETEFGKLDGLLHNAS
                     IIGPRTPLEQLSGDNFMRVMQVNVNAMFMLTSTLLPLLKLSTDASVVFTSSSVGRKGR
                     AYWGAYAVSKFATEGLMQVLADEVDGIASVRANSVNPGATRTDMRAQAYPGENPQNNP
                     LPEAIMPVYLYLMGPDSTGVNGQAFDAQ"
     gene            complement(36876..37547)
                     /gene="mupP"
                     /locus_tag="VFF22_13905"
     CDS             complement(36876..37547)
                     /gene="mupP"
                     /locus_tag="VFF22_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017906402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate phosphatase
                     MupP"
                     /protein_id="HZX17940.1"
                     /translation="MRLRAVLFDMDGTLLDSAPDFVAISQVMRAERGLPPIADKLIRD
                     QVSGGARAMVAANFAMDPEAVGFEALRLEFLERYQSHCAVLTRPFDGMNELLDDIEQA
                     KLIWGVATNKPVRYAEPIMQQLGLAQRSAVLVCPDHVSRSKPDPEMILLACAKMGVEP
                     AATLFIGDDERDIEAGRAAGCKTAAVTYGYIHPQDNPRNWGADVVVDHPLELRTVLER
                     ALCSC"
     gene            complement(37553..38251)
                     /gene="ubiG"
                     /locus_tag="VFF22_13910"
     CDS             complement(37553..38251)
                     /gene="ubiG"
                     /locus_tag="VFF22_13910"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003172645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HZX17941.1"
                     /translation="MSNVDHAEIAKFEALAHRWWDRESEFKPLHDINPLRVNWIDERV
                     GLAGKKVLDVGCGGGILSESMAQRGASVTGIDMGEAPLAVAQLHQLESGVSVEYRQIT
                     AEALAEEMPEQFDVVTCLEMLEHVPDPASVIRACYRMVKPGGQVFFSTINRNPKAYLF
                     AIIGAEYLMRLLPRGTHDFKKFIRPSELGAWSREAGLEVKDIIGLTYNPLSKRYKLES
                     DVDVNYMIQTLREE"
     gene            complement(38615..40255)
                     /locus_tag="VFF22_13915"
     CDS             complement(38615..40255)
                     /locus_tag="VFF22_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017679062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HZX17942.1"
                     /translation="MSRLLCTLLLGLLALNVSAQSLTASVDRTRLDAGESVELTLESD
                     DATLFGKPDLQPLNELFEVLGTRQVNRLTSGNNGAQASTRWIVTLQPKHSGYVVIPPL
                     SLGQLQSEPITLHVQQVSSADDSRLAPVFIDASLDQESVYVQAQTVLTLRIYHSVSLY
                     DDSSLTPLDMPQARVEALGEPRTYEKDINGVRHGVIELRYAIFPQESGELLIPSQAFT
                     ATAVDRSASNAYNPFGPRPGKVVRVRSPEIPLTVKAKPASYPADAPWLPARALSLSEV
                     WSPQPETARVGDSLTRSLLLKVEGLASAQLPPLPATQVAGLRRYPDQPQLANQASDNG
                     LVASREEREALVANREGVIALPAIEVLWWNTTTDQLERSSLPARNLQVAANPNLETLP
                     VEATRPAVSEAANVQLWPWQLSSALLSLTTLLGFTLWWRARRQPPVLPSQQTGPSQRN
                     LLDDLKRSCLANDSQATRHALDAWARQQPETLADMAARFTPLSDALDGLNGALYSESG
                     QHWQGRNLWLAIRSLPAVEDLESTTNPDTSALPPLYPR"
     gene            complement(40252..42000)
                     /locus_tag="VFF22_13920"
     CDS             complement(40252..42000)
                     /locus_tag="VFF22_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZX17943.1"
                     /translation="MTSLWPHWQRPFWLLLIPLLGILLWQLWHREKRTGRWQALLPAA
                     FQAALLTATGGRNSRLPWLALGLAWLLALLALLGPSWQRIEQQNPKPADPLVVLLELT
                     PEMLASDASPNRLAQAKRKLLDLLEARQDAQTAIVVYAGSAHTLVPLSDDLATSRNLL
                     DVLSPEIMPEPGKRADLAVAKALQLLEQGAQGNGRLLLLTSSLDETQRQAITQALGRR
                     GERLRILGIGSTQGAPIIQEDGSFLKDAQGSILLPRLDSNGLKRFASELGGLYQGVTL
                     DERDLRNLGLLDAPQQLRRDGELTRLQAWADQSHWLLLPLLLLAACAARRGWLLCLPL
                     LLLSLPQNSYAFSFDDLWLRADQQGQRLLEAQQPAAAAQRFHDPRWQGQALYQAGDYA
                     AAAERFANGETASDHYNRGNALARSNELEAAVDAYTQALELQPDLAQAQKNKALVEEL
                     LRQNQQQQNQADNPQEPNEQQNQPSSQPQAGQSDPDAKQQAEQSPAKPSEPEDKGEKP
                     AQPGSDEDADTPPKQPEEDDSDSGEEQIASAEPALDGERRQALEQWLRQIPDDPGELL
                     RRKFRLEQQQRQEQLQ"
     gene            complement(42006..43037)
                     /locus_tag="VFF22_13925"
     CDS             complement(42006..43037)
                     /locus_tag="VFF22_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZX17944.1"
                     /translation="MFEFAWPWVFLIAPLPWLLRAWLPPADSGEAALKISFLAELEGL
                     SGRRARVRLPALRQQAPFALIWLLLLLASARPQWLGEPLPLPASGRDLLLAVDVSGSM
                     DYSDMLWEDQEVSRLTLVKHLLGDFIDGRQGDRVGLILFGSRAYLQSPLTFDRQTVHT
                     WLDEAQKNIAGPQTAIGDAIGLAVKRLRERPAQSRVLVLVTDGANNGGEIDPMTAARL
                     AADEGITIYPIGIGADPEQGGVLGMLGFNPGIDLDEPSLRAIAEATGGEYFRARDSEE
                     LQAIEATLDRLEPVAQKPTLARPTLPLYPWPLGLALLLSLLLVSQTLWPSLQQRWRNQ
                     LTLLRGKQS"
     gene            complement(43030..43524)
                     /locus_tag="VFF22_13930"
     CDS             complement(43030..43524)
                     /locus_tag="VFF22_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HZX17945.1"
                     /translation="MNPLDQLEPLIAPAPISWWPPAPGWWLLAVLLPLLLWAGFKLLK
                     RLPRKAVAQSAEAPLDPLRQAALNELERLSKPYDGVDAGPWLQQLNAILKRLCRERYP
                     QSHSHILSSRAWLAFLDSRCPAAGLTRWMILVEGAYRPHCNLDDKAIAGLNQAVSIWI
                     RKHV"
     gene            complement(43521..44462)
                     /locus_tag="VFF22_13935"
     CDS             complement(43521..44462)
                     /locus_tag="VFF22_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011334590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZX17946.1"
                     /translation="MHDQPTQPGIRVSLGELIEMRHRVREVQLFSTPARRSPLIGLHH
                     SKLRGRGVDFDQVRVYQPGDDVRTIDWRVTARTQEPHTKLFHEERERPIYIMVEQSKR
                     LFFGSGLMFKSVLAAQAASLIGWAALGHNDRIGGLVFGDLEHHEIKPRRSKQSLLQLL
                     SRLAKANQALSNDSQGGRDSFGLALRRAREVLRPGSLAVILCDERTLSDSSEQQLLLL
                     ARHTDLLLLPLSDPLDHALPNAGLLRFTEHGAQLELDTQDAELRQAYRSLADARQARW
                     QRLAQKLGVPLLALNTQSEMIEQLREHLNAQRPRKAL"
     gene            complement(44468..45427)
                     /locus_tag="VFF22_13940"
     CDS             complement(44468..45427)
                     /locus_tag="VFF22_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZX17947.1"
                     /translation="MEHREALVALRHFLSSQILGQEKLIERLLIALLADGHMLVEGAP
                     GLAKTKAIKELAEGIEAEFHRIQFTPDLLPADITGTEIYRPETGSFVFQQGPIFHNLV
                     LADEINRAPAKVQSALLEAMAERQVSVGRSTYHLSPLFLVMATQNPIEQEGTYPLPEA
                     QLDRFLMHVKIGFPDASVERKILAQARGEALNGETKPEQLVSQQAIFAARQEILGLYM
                     ADAVEEYLVQLVMASRTPAKFDPELAEWIAYGASPRGSIALDRCSRAHAWLAGRDFVS
                     PEDIQAVLFDVLRHRIILSFEAEAAGIDQDRVVQRILDVVAVA"
     gene            complement(45679..47559)
                     /locus_tag="VFF22_13945"
     CDS             complement(45679..47559)
                     /locus_tag="VFF22_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX17948.1"
                     /translation="MQFFLSWQQKFRLLIVITLLSLGLMTTASLWANKQLSESLQARE
                     DAKGYASATTSLVNDWLKLTALRQQLTPDTLAQYQQRLDALEEHAQALLTNAEQLLDP
                     AVSQSAATIVASLRDELSQQRAWLALNQQLGLSPFEGKRKTLAEAAKALEPITIGLIR
                     SFIASALSGQRDYLSTFDPVYAEQAKTAIDELQAKVVELEWQDNTIGQSVTRFSQAFT
                     EAGGLIQQIRGSNQRLKQLGQQIEQQIDSQGSMLEDGILARTDQRAQQARNSANWILG
                     LSFVVVALFLTLTLNQASRTLVTRLQSVTALMSQVASGNLTGSLNVGNNSKDEFNQLG
                     DASNRMITGIGHIVGQVVEANRELSQLHRYLSDSMRSLGDNSSQVEIQTEQAASASQQ
                     ISATINEMARRTSDVGNATHTAYDSARQGSIIIEASVESMRRLSQLIQATHAQVALLG
                     QSSNKVSGIIDVINSLADQTNLLALNAAIEAARAGDAGRGFSVVAEEVRSLAQKTVSA
                     TTNIAVIVSEFKEQTHNMHELMSSGISLAAESEQHAAQVAASISQITCSMEQLSGEMN
                     QVVVAIEEISSTTEDIAGKIEEINVHTGETKNLRMTLDQHTRGLSTQVETLSLSARQF
                     QI"
     gene            48073..>50656
                     /locus_tag="VFF22_13950"
     CDS             48073..>50656
                     /locus_tag="VFF22_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HZX17949.1"
                     /translation="MAFFTAASKADFQHHLQTALAQHVSEQALPQVALFAEQFFGIIA
                     LEELTQRRLSDLVGCTLSSWRLLERFEPAKPEVRVFNPDYEKHGWQSTHTAVEILHSD
                     IPFLVDSVRMELNRRGYSIHTLQNSVFSVRRNKSGELQEILPKGAQGKDVQQEALMFL
                     EIDRCANVGELKTLEKDLHEVFSDVRLSVADFQPMKAKAKELLAWLDKAKLKVEGAEL
                     EEIKVFMSWLLDDHFTFLGYEEFTVADSAGGGSMVYDEKSLLGMSKRLRSGLKADELH
                     IEPEAVAYLREPQLLSFAKAAVPSRVHRPAYPDLVSIRELDAKGKVIKECRFMGLYTS
                     AVYAESVWNIPFIRRKVAVIKQRSGFDSSAHLGKELAQVLEVLPRDDLFQTPVDELFN
                     TTMAIVQIQERNKIRVFLRRDPYGRFCYCLAYVPRDVYSTETRMKIQQVLMERLQATD
                     CEFWTFFSESVLARVQFILKVDPKNRVQIDPLRLEKEVIQACRSWKDDYTSLIVESLG
                     EAQGTEVLSEFPGGFPAGYRERFAPHSAVVDMQHLLSLNSERQLVMSFYQPLTQGGEQ
                     QLHCKLYHADTPLPLSDVLPILENLGLRVLGEFPYKLRRADGREFWIHDFAFTYAEGL
                     DVDIQQLNDTLQGAFVNIVGGAAENDGFNRLVLMAAMPWRDVALLRAYARYLKQIRLG
                     FDLSYIASTLVNHADIAKELVRLFKTRFYLARKLTADDLEDKQQKLEQAILAALDNVA
                     VLNEDRILRRYLDLIKATLRTNFYQTDAAGQNKSYFSFKLNPRAIPDIPRPTPKFEIF
                     VYSPRVEGVHLRFGDVARGGLRWSDREEDFRTEVLGLVKAQQVKNAVIVPMGAKGGFI
                     PRRMP"
BASE COUNT         9138 a        14663 c        16534 g        10321 t
ORIGIN      
        1 ggccctggcc acgcgggtgg cgcagagcga ttcgacggta ttgatcacgg gcgaatccgg
       61 cacaggtaaa gaggtgctgg cgcgctttat ccaccagcag tcgccacgcg gcagcaaacc
      121 ctttatcgcc attaactgcg cggcgattcc ggacaacatg ctcgaggcca cgctgttcgg
      181 ccatgaaaaa ggcgcgttca ccggtgcgat tgccagtgcg ccgggcaagt tcgaactggc
      241 cgagggcggc accattctgc ttgacgaaat ttccgagatg ccactgggtc tgcaggccaa
      301 gctgctgcgc gtgctgcagg agcgtgaggt ggagcgggtt ggcgcgcgta agccgatcat
      361 cctggatatc cgcgtgctag ccaccagtaa ccgcgatctg gctgcggagg tggcggcggg
      421 gcgcttccgt gaagacctct actatcgcct gtcagtgttt ccgctggcct ggcgaccgct
      481 gcgtgaacga ccggcggaca ttgtgccgtt ggcggagcgg ttgttggcca agtacgttaa
      541 gaaaatgaac cacgcgccta tccgactgtc ccctcaggcg cagatgagcc tgatgagcca
      601 tagctggccg ggcaatgtgc gtgagctgga taacgccatt cagcgcgcgt tgattttgca
      661 gcagggcggt ctgattcagc cgcaggatct ctgtctgttg gcacctattg gttcggccgt
      721 tacgccgccc gcgcaaccgc tgttggcggt ggtggtcaat agcgcaccag cgcagcagga
      781 cgtctcgccg gcagaggcgg gggccttggg cgatgacctg cgccggcatg agtttcagat
      841 gattatcgat accctgcgct ccgagcgcgg ccggcgcaag gaagccgccg aacgcctggg
      901 catcagcccg cgcaccttgc gctacaagtt ggcgcagatg cgtgatgccg gcatggatgt
      961 ggaaggctat ctatttgcca gctgagccga ttactctcgg tagttgtgct tgcaggttgt
     1021 aggggcggcg gggacgccta gcccatgccc gcgaagtcgc tatcgcgggc atggcccgct
     1081 cctacgccct ggtcgcgagt gctcgtgtag cccggatgca atccgtggtc ctcggcattt
     1141 ccccggatta catccgggct acgactctga ttaatcctgc cacggcatgg gcgcacccaa
     1201 cagacggccc atggcgccgt acagccccag ctccttgagc actgccagct cgccctctgt
     1261 ctccaccatc tcggcaatca atggcagatc gatgctgttg gtggcgcgga acactgcttc
     1321 gataaacatg cgtttgtcgc tttcctgatc gatggctcgg atataggtgc cgtcgagttt
     1381 caggtaagcc aggcccaggt gggtcaggtt gccgatcagg ctgaagcgcc cgccgaagtg
     1441 ttgcaggccc aggctgaagc cggtgtcgcg gatgccctgg ctaagcgctt ccagctcggc
     1501 cgcaggcggc agatggcgct cgtctacttc cagagtcagc aggctagcct gctgtgggtg
     1561 ctgcctgagg atttcgaaca tgcgcttaag ggtttctgta tcgcgcaggg tcgcggccga
     1621 taggctcagg gccagtggcg ccgcatgctg gctgaggtgc gccaggctgt gttcaagcat
     1681 ggctaagtca aaacgcgcgg tccagcccag acgttcgatc cagggcagga agcgcccagc
     1741 ggcaatcgct tcaccctttg gatcaagcag gcgtgccagt accttgtggt gcagtagtcg
     1801 gttgcgatcc gcgcattgcg tgaccggctg gaaataaagc tgtagcttgc gtttgttcaa
     1861 cgcgtcgtcg atccaggtgc gccattcgtg caggccctga gttggttggg cgttgtagtc
     1921 atccaggcgc acccagtcgc ggctcgggtc gttctgcgct tgcgcaaggg cctgatcggc
     1981 gcggccgatg acctggctgg gttcttcgcc aggacggaag gcggcaatgc ccatatgcgc
     2041 cacgggcgta cagtcactgg cgccggtctg gcgcaggctt tccaggccgc tgtgcagctc
     2101 accggcaagt aggtcggctt cttcactgcc aatgcctggg gccagcagac tgaactcgcc
     2161 gccacgactg cgtgaggcca gccagtccga gcggttggtc tggttaagca ggcgcttgag
     2221 cagctcgccg atggcggcaa tcagagcgtc ggtctgctgg ccgccgaggc gctggttgag
     2281 tccgccgagg tcgttgaccc gcagcagcag cagatagcct tcggcattct gctcactgac
     2341 caccaattgg tgggccaggc ggatatcgaa caaacggcgg ttggccagac cggtgatgct
     2401 gtcctggtag gcctcttcac gcaatttttc gctgcgtgcg gcttcttcgg cgaacagggt
     2461 tttcagtttt tccaccatct ggttcatggc ggtgactacg cgcttgagct cgggtgtgcg
     2521 tggcaccttg ggcagggtga ggaattcgcg gcgggtgatg gcttgggctt gttgcaccat
     2581 gttgtccagt gggcgcagtt gagtgcgcag caaccagccg ccgagtacgg cgctgaccag
     2641 gccgcagagc agcagccaga tcaggctgcc gatggcgctg tcccagagtt tggccagggc
     2701 gaattgcggg tggctgagta cctcgactcg tgcagcctgt tcccagccgc gcatgatcag
     2761 cgcatcgccg ccctggggct gcaggttgac cagctcgctg aaccagaccg ggacggtttc
     2821 ggcactggca tcgtcgctgc gtcgttcgac tatcacttcg ttattgggga tgcgtatcac
     2881 acggatgctg gtgaaataac cgctgtcgaa gatcgagctg accatcagtt cgatcattgc
     2941 cgggtcatcc acgtgtggcg tcatcgacag gcccaatgcg gttgcggcgt cctgggcgtg
     3001 ggagcgcagc tggctgagca gttgctcgcg ggagctttcc actccggcga taaaactccc
     3061 agtaaaggct acgaccagga acaggcagat ggcgagaaat agctgcttga gtagagacat
     3121 aggttcctcc tagccttcgc cgatggcgaa gccctccgtg cgcatttttt tcaagatgtc
     3181 ctgccagcga gagagcttct tggagtcgct ctttctgctg gtggaacccg ggagatagag
     3241 gccttcggcg ttgaatgcat agaccggcag caggtctttg cgctgcgagg caggttttat
     3301 ttcattgatc aggttgtcca gcaccagtgg ctcggcaccg gggctggcgt agtaggtcag
     3361 gaccatatgc gcctgattgt agttgagcgc cttgacgtag gtgatacgca gcttgtcact
     3421 ggggatgccg aggcggcgca gagtgaagta cttggcgatg gaatagtctt cgcagtcgcc
     3481 ttcgcccttg accagcatct cgatgggtgt ggcccagtaa tcgttctgtt tccacaggcg
     3541 gatatcgtcg gtaaagcgga tttgccgatt gaagaagcgg ttgacctcgg tgagctggtc
     3601 tctttcgctg ctgctgtcac tggccttgat ttgcgcttcc caggcctgga tgcgctgctt
     3661 ggctgcgccg aggttgccgt agcggctttc ggcattttgc aggatgacgc cgaagtccca
     3721 gttggccagg gcgctggcca gaccgatcag caggatgcag ccgcccagca acgctcggtt
     3781 gaggcgcttg aaccatccat gagtgagctg acggggtggc atgtaccgag cctctgccag
     3841 ggattggcaa agtctaggtg ttgctgctga tttttgccgc agcggactat agcgaatgtt
     3901 gacgggtgtg ttgcttaggg tttgatcagg gttttctgac tgagaatgta caggctaact
     3961 agcaccacgc tggtcagcat aaaggcccgg gcccagaaca atggcaccag gtagcacgac
     4021 aggccgatgc tcagccacat cacgccaatg gcgtagacct tacccttgag cggaataccc
     4081 tgaccttcga ggtaatcgac gatccacggg ccaagccggc ggtgattgac cagccacagg
     4141 taaaagcgct tcgagctgcg cataaaacac gcggccgcca gcagcagaaa cggtgtggtg
     4201 ggcagcaccg gaaggaatat gccgataaca cccagcgcca cgctaagcca gccgatggcc
     4261 agcagaacgt agcgaataat cgggttgcgc tgctcttgaa caggcggcac ctgaatttca
     4321 cgcgccatgg cgcgtgttta gtggtggcga ggtttcagga gggccggttt ttcttccggc
     4381 gcattgcaca gcaggaacag tgcggtcagc agctcgggga tctgctcgac catgctgtcg
     4441 accaggtcgc ggtcacgggc gatctcggcg aactcgggct gttcatcgaa caggccggaa
     4501 ccgaccatga tcggcagcag caactcgctg acttcatctt cggcgtcgtc gaaccacacc
     4561 gattcacgca ggaacacgcc ttccataaag ccgatgcacc agccgcgcag gtcggagtcg
     4621 tctggctctt cgcccagatc gaggtcgcac ggtacgtcgg gctcatcgtc gctggccagt
     4681 tggcgggcga tatggctttg cagctggatc agggtggctt caatctcttc gcgctcggca
     4741 tcactgcggt agtgcggcgg ctcggagaac agtgcgtcaa tccactcgcg ctctggcacc
     4801 ggctgtgggc agatcgacag ggcagtcagg tagccgtggg cggcgatgta gtccagtgcc
     4861 tcgtcatgca gctcatcggc atcgaggaag gcttgcaggc gggacaattg ttcggcgaaa
     4921 gacatcgggg gctaccttga agaagtaaac gaggctggat tctagtccag acccgttaca
     4981 gcggccatag ttctaggtaa atacgggcaa atgcgcagca cagtcagatc gggactcagg
     5041 cccattgcca ggtgcgtata atgcgcggct tgtttcggag acctttcatg ctcgagcacg
     5101 cacaacgcat cctcaaagac gtattcggtt acgacagctt tcgtggccgt cagggtgcga
     5161 ttatcgagcg cgtggccagt ggcggcgatg ccctggtgct gatgcccacc ggtggcggca
     5221 agtcgctgtg ctttcaggtg ccggccctgc tgcgcgaagg cctggcggtg gtggtctcac
     5281 cgctgatagc gctgatggac gatcaggttg cgacccttga cgagctgggt gtggcggccg
     5341 cagcgctgaa ctccaccctg aacgccgatg agcagcgtga gattgccgag cggatcaagc
     5401 gcggcgaaat caagatgctc tacctggccc ctgagcggct ggtgcagccg cgcatgctga
     5461 gctttttgca aaacctcgac attgccctgt tcgccatcga cgaggcgcat tgcgtgtcgc
     5521 agtggggaca tgatttccgg ccggaatacc tgcagctggg ccagctcgcc gagctgttcc
     5581 ccaatgtgcc acgcatcgcc ctgactgcca ccgcagatat gcgcacccgc gaggaaatcg
     5641 tccagcgtct gcatctgcag aatgccgagc gttttctgtc gagcttcgac cgaccgaata
     5701 tcttttaccg tatcgtgccc aaggagcagc cgcgcaagca gctgctgaac ttcctcgcgg
     5761 cgcgcaaggg cgatgcaggt atcgtctatt gcctatcgcg caagaaggtc gaggaggttg
     5821 cctcgttcct ctccgagcag ggtttcccgg cgttgccata ccacgccggc ctggccaacg
     5881 agttgcgcgc ctatcaccag aagcgctttc tcaatgagga agggctgatc atggtggcga
     5941 ctatcgcctt cggcatgggt atcgacaaac ccaacgtgcg ctttgtcgcc cacctcgatt
     6001 tgccgaaatc cctggaggcc tattaccagg aaaccggtcg cgccggccgt gacggcctgc
     6061 cggcggatgc ctggatggcc tacggtctgc aggatgtgat ctttctcaag cagatgctca
     6121 acaactccga gggtgacgag cgccacaaac gtgtcgagca gcacaagctc gatgccatgc
     6181 tggcgctgtg tgaggaaacc cgctgccggc gccaggcgct gctggcctat ttcgatgaag
     6241 ttatgccgca gccttgcggc cattgcgaca actgcattga tggtgtgcag acctgggatg
     6301 ccaccgagcc ggcgcgccag gcgctgtcgg cgatctatcg cactggccag cgctatggcg
     6361 ttgggcacct ggtcgatgtg ctactcggtc gcgataatga aaaaattcgc ggcgtgggcc
     6421 atcaacacct ggcggtgttc ggcatcggca aggcgctggc cgaaggtgaa tggcgttcgc
     6481 tgttccgcca actggtggcg cgcggcctgg ctgacgtcga cctcgaaggc tttggtggcc
     6541 tgcgactggc cgacagttgc cggcccttgc tcaagggtga ggtgacgctg cagttgcgcc
     6601 gtgatctgaa gccgcagcaa agcgccaagg cttccagcag cgccgccagc cagctggtgc
     6661 gcggtgaaga acgcggtcag tgggaggcct tgcgcgcctt gcggcgcaag ctggcggaag
     6721 agcatggcgt gccgccttat gtaattttcc ctgatgccac cttgctggaa atgctgcgca
     6781 gcaagcccag taccctggcc gaaatggcgc gggtcagtgg cgtcggcgcg cgcaagctgg
     6841 aacgttatgg tgaggccttc ctggccgtgc tcggtgcgga tgatgaagcg ccgcgtgcgg
     6901 tggtcgactt gcgtcatgaa ctggtcagcc tggcccgtgc cggcatgacc ccggcgcaga
     6961 tcgccagtca gctcagctgc agcgagaaga atgtctacgg cctgctggcc gaagccattg
     7021 gtcgtcagca gctgtccctg gaacaggcgc tggatgtgcc cgaggcatta ctcggcgaga
     7081 ttcaggacgc ttttctcgat ggcgaaggtg aattgccgcc ggtagccgcg atcagcgagc
     7141 agtttgtcgg acgtgttgag ttgggcatcc tgcactgcgt gcgtgctgcc ctgcaggcag
     7201 agttcgaagc cgagtgattg tggggtctgg cagccgggtg atcttgtggc cccgggcgcg
     7261 agcggctaag gtattttcag ccactccgct gtagaggttc cggtgccgct ttcccatgcc
     7321 tggctagacg atgttagccc cagcccctat gcagatcagc tctctttggg cttccgcatg
     7381 ttgcgtttct cccggccact ggagcaggag tatcgcgact atgtgcgtga cgacaccttc
     7441 gacctcaaac gcattgccct gggtgttggc attttggtgt ggctggcttt tgccgcgttt
     7501 gatttcgtcc tgatcaaagc gcccgaggta tggtggatgt tcgccatccg cctcgccgtt
     7561 ttactgttgc tgtgtctatt cggcgggctg ctgattcagc gcaagcatgt tcatctgttg
     7621 caaccgctga gcctggtcag cctgttagcg ttgggcgcgg cggcgtcctt tgtggttggt
     7681 attgctcatc gggttgatcc tagctacccc tatgagggac tgttgctggt ctgtatggcg
     7741 gcgtacttcc tggcggggtt acgcctgatt gaggctttgt gctgctcatt gctggtgctg
     7801 ctggtatatg tctgcgtcga gctgctggtg gccggcttgc cgctgccgcg tttgctcaat
     7861 aacactctct ttctgttgct cggcaacctg atgggcgcgg tgggttgtta tttgctggag
     7921 ttcaagtcgc gcgagcattt tctgatcagt cgattgatgc gcgtgttggc cgaccacgac
     7981 agcctgactg ggctgcacaa ccgccgtagc ttcaaccgtc agttcgagcg cttgtggcgt
     8041 caggctcagc gtgaacaccg tagcctggcg ttgctgcttt gcgatgttga tcacttcaag
     8101 gcctacaacg atcactacgg ccaccaggcc ggcgacaacg tgttgcagcg cattagcagc
     8161 ctgctgcagg acgccgcgcg gcgcccgctg gacatggccg tgcgcctggg cggtgaagag
     8221 tttgccgtgc tgctctatga catcggtgag gtcgaagcgc ggcgtcacgc cgaagcactg
     8281 cgtgagaccc tgcagaccct gaatatcgaa catgccggct ccgctacggc gagcatgctg
     8341 accatgtcga ttggcatttc ctgcattcag cctgccgtgg gtggtgcgtt ggcgcagctc
     8401 tacgagcacg cggatcgcgc tctgtacgag gccaaggcgt ttggccgcaa tcaggtcgtg
     8461 gcttgagcgt ctaggggtta ggtggccgat tttgccgctg cgcttggcgt ctgtggtgag
     8521 tgctcggggt gaagtcgcgg tgctagcatc gctgaggttt tttccttgag tgattgctcc
     8581 gatggatcga ctgattcagg ccaacggcca gccgcattac ggtatttttc ccgccgcgcc
     8641 agggctgatc aattaccgcg attttgattt tcgctcgccg atggggcgca agctgggcgc
     8701 cttgtccaag tggcgacgct tccaccaatt tcagtatttc ggcctgatca gtgatgagct
     8761 gattggcggt tgcgccctgg ccaacctcag cctgctgggc gtgggctttg tctacctgtt
     8821 tcatcctgcc agtgggcgga tgattgagcg ccagttcaag ctgccgctgg gctttggcag
     8881 ccagttctcg cagacaccgg atcagggcgt gtgcgaggtg cgcagcggcg gcaatctgct
     8941 gcgtctggag aacatcgccg catcgaagga aaagcgtctg ctggtcgagc tggatgatgg
     9001 cacccgcatc gatgcctgtt tttccgaggc agagcctgct tttcagccga tgtgcatcaa
     9061 cacgccgacg gcggtcaatg gctgggtcta tgcgcaaaag gtagcggggg tgcgctgcac
     9121 ggggcaggtg cgcagcgcgc tgggggaatt tgatctggcg cagatcggcg cattcgctca
     9181 tcacgactgg tcggccggtt atatgcggcc tgaaacccat tggaactggg cttgtctgtc
     9241 cggcttggcg ggcgagcagc gggtagggct gaacctgtcc tgcggggtca atgaaagcag
     9301 ttttaccgag aactgcttct ggcttgatgg cgagttgctt aaggtcgata ccgtgcgctt
     9361 tgcctatgat cgcgatcact cgctagagcc ctggttgatc aactcctttg acggccaggt
     9421 gaagctgcgt ttcgaggggc gtggcctgca taaggagcgg ctgaacgtgg ggctgctggc
     9481 gagcaacttc aagcagatct tcggccagtt cagcggcgta ctgcggccga ctggccggcc
     9541 cgaagtgcgc atcgacaacc tctggggctt tgtcgaagac cagtatgtaa agtggtgaaa
     9601 tcttttacag gcttgcctcg ttaaaagggt tatggttagc tggctaataa ttagtttttt
     9661 atagttaaga gccgttgtcc atgtcgcata ccgatcaaca ccgttttgcc atgcaagttg
     9721 cccaactctc ccgtgcctgg cgcgccgaac tggatcgccg tctagtcggt ctggggcttt
     9781 cccaagcccg ctggctggtg ctgctgcacc tggggcgctt tgccgaatta ccgacccagc
     9841 gcgagttggc gcagagcgtt ggcgtcgaag ggccgaccct ggcgcgcctg ctcgacagcc
     9901 tggaagctca aggtctggtc agtcgccagg cggtgcccga agaccgccgc gcgaagaaga
     9961 ttgcgctcag cccacaggct cgtccgctga ttgaaaagat cgaagcgatc tccgcgcagc
    10021 tgcgtcagga ggtctttgcg ggcattgatg aagacgaatt gcgtcgctgc cagaaggtcc
    10081 acgctcaggt gttgagtaac ctggaaaaac gctgatgcgt gacgaccttc agagcctgca
    10141 gcaacgcttt ggcccgcgtt acccgcagtg gctgctggcg ctgctgatgg tcggcagcat
    10201 ggccatggta ttggcctcca ccagtatcaa cgtcgccttg ccggcgatca tggcggattt
    10261 cgccatcggc cggccactgg cgcagtggct gtccaccggc tttctcgcgg ccatgaccgc
    10321 tggcctgctg ttggccgcct gggcccaggc gcgtttcggt gcgcggcgta cggcgcaatt
    10381 cggcctgctg ctgtttgtcc tcagttcact gttggcgctg gtggcgcacg ccgcctggca
    10441 gctgatcgcc ctgcgtatcg tgcaggggct gtgtgcggga attgtgcagc cgttggccat
    10501 ggtgctgatc tttcgtgtat tcgccgatgg tgggcgaggt atggcgctgg gcatatacgg
    10561 cctgggggtg atgctggcgc cgtcgctggg gccggggatt ggcggctacc tggtcgatca
    10621 ttttggctgg ccgtcgatct tctggatgcc gctgccaatg tgtctgctgg ccatgctcgc
    10681 cggacagtgg ttgttgccca gtgaacgtga gcgcagcaca ccgcgtctgg atatccaggc
    10741 cttcgcctgg ctgtgtgtgg cgatatttgc cgcgctgggt tttctggccg aggcgcagcg
    10801 ttttgcctgg tcggcgccgc gggtctggtt gccgggttgc ctggcactgc tggcgggcgc
    10861 ggcgtttatt ctgcgcagcc ggtctgccgc tcagccgctg ttgccgcttg cgctgtggcg
    10921 acatgccggg tttcgcagcg ccagttgggt cgccttgacc cttggccttg gcctgtatgg
    10981 ttcgacctac ctgatcccgc tgtatctgca gaccgttgaa ggcttcagcg ccgggcgctc
    11041 gggccttctg ctattgccgg ccgggatctt gatgggcatg gcgtcctttc tcggtggttg
    11101 gctcagtgac cggctgtctg cggccgtgct gctgagtgcc ggtctgctga tctttgcgct
    11161 gtcggcagcg gggctggcct gggtcgagca gggcgcctca tttatccagc tgtgtttctg
    11221 ggcctgtgtc gggcgcattg gcctgggcat attgctgcct gccttgagta ccggttcgct
    11281 ggatattctt agcccagcgg aattggcgca gggcgcaggg gccattacct tcgtgcgcca
    11341 gctgggcggc gcgttcgggg tcaatctact gaccttcttt ctggagtggc ggcacagcgc
    11401 tgaaggtggc gaccatttcg cggagatgct ggctttcaaa cagagtttct ggttggtggc
    11461 cggtgtattt gcgatcacgg ttttggcggc ctggggcgtc aaatcgacaa gacgctgaca
    11521 gctaaacggc aacagaccct tatgtgacgg agttgtcatt ttcttgactg caggcctatg
    11581 cttgcaggtg taatggcttt ttgccaaacg gcgggtggca ttttctgcat gcagtgcgcc
    11641 gtaattattg ccatgcacca gccgggttaa cacggaacgt agtcccaagg ccgcatttgc
    11701 cgtcgtctat actctcgcaa gcgctggaag cgcacgtcca ttacatgcag tggcgagagt
    11761 gcagggacgg tttcatcggg agcgttttta tggcaaaggt tcgtcaatta ctgataggcc
    11821 tcgcatccag ctcagccctt tattcggggc tggttccagc acttggcctg ggtgaaatca
    11881 ccctgcattc ggcgctcagt cagccgctgg atgccgaaat cgaattgttt gatgtgggcg
    11941 acctgagcga cgccgacatg cgcgtgcgcc tggcacctgc cgaagtgttc agccgttccg
    12001 gggttgatcg tctgtatttt ttcaatgatc tgcgcttcac cccgttgctg cggggcggca
    12061 aaagtgtgat tcgcgtggtg tcgacccagc cggtgcgcga gccttatctg aattttatcg
    12121 tcgaagtggt acggcccaat ggccagttgt accgcgagta caccgtgctg cttgatccac
    12181 ccagctcggc cgcctaccgc agcctggctg cgcctgcagc atctgccgcg ccagctgccg
    12241 tagcgagcgc gccgcgtgcc accactgtcg agcgtgcgcg ctcggcagta ttgcccgccg
    12301 cgcagcaggg caatcgctac agcgtggtca gcggtgacag cctgtggaag atcgccacgc
    12361 gcttggccgt cgatgccggg caggtggcgc ctcaggcgtt gatgaacgac atccatgcgc
    12421 tcaaccctca ggcctttatc aatggcgata tcaatcgcct gcgtgccggt gcgcaattgc
    12481 tattgcctga cagcgtcgcc gattcgtcgc cttcagtaat tgagccggct gcaccaatta
    12541 ctacgccggc cgttgcgccc ccagagattg cgccaacagt cagtgctgag ctgccggttg
    12601 atccgcctgc tgagctgttg atccaggagc aggcccgcgt cgagcaggag ttggctagcc
    12661 agattagcga gacactgcaa ttgcagcaga gcctggcgca gttgggcctg caggtgcagc
    12721 agctgcagga gcagatgggc gcgcgggatc agcagattgc cggcttgcag gccgagttgg
    12781 cggcccgtcg ggcgagtgcc gaagcaattg ccgcgcctgt gtctgcaccg gtcgcggaaa
    12841 acccgtcggc tacggccgag tcaggcaaca actggctggc gattatcgcc ggcctgctga
    12901 cgctgttact ggctggtgtg gccggcctgt tctggcgtgg caaaaaacct gtcgagcctg
    12961 cgcccgtgcc tgcgcagccg gcgcgagcag cgagcatgcc caaggcgcaa agtatggcgc
    13021 cggctttacc ggtcgcaccg gcagcagtgg cccttaacgt acgtgacggg ccggtatggc
    13081 cggcagcggg tgaagatgcg ctgcaaagtg ccaacgtgta tatcgcctat ggccgcctga
    13141 gtgaggccgc tagtaccttg cgcaaagcct gggaggcgca gccgcagcgc agtgatattg
    13201 gtttccgcct gctggaagtg ctggccttgc tgggtgatgc tcagggcttt gtcgcgcatg
    13261 aggtattggt acgtgcaacc ggctttaccc ccgcgcggat cgacgaatta aaagcgcggc
    13321 atgccgagct gttcagcgcg ccgccgagca gtctgctgga cgatgtggtg ctggaactgg
    13381 atgaagtcga ggcacagccg ttagtggatg ccgccaatga tggtcagctc aatctggacg
    13441 atatgtcgct ggatgccgac tgggacctgg tcagcccctt tgccccgact gcgccaaaaa
    13501 aagtggcgaa caaggccgag agcaatgctg cgaccaagcg caagacctcg gatatcttcg
    13561 agctgtccgg cagtcgtgat gcacgcagcc cgtacgtcga gtcgatgctg gtggaggaaa
    13621 cctcccacga cggttggggt ggtgacgtgc ttgatgtggc tctgctggat gaccttgatg
    13681 agctggccgg caaccgcgac aacctgtcca aactgaatat ggccctggcc tatatcgagc
    13741 agggcagtct ggacagcgcc tgccagatcc tcaatgaagt tatcaacgag ggcgatgaag
    13801 agcagaagca ggaagcacgt cagctactgg ccaaaatcgc ctgattgaaa gcgctgttgc
    13861 ttaacgcccg ctagcctgcg ctacgcgggc gtttttcttg tgctgaggta aggccactgc
    13921 gcgttatcat cggttttttc atccgccgcg agcctgccaa tgtccagccc gaaagccgaa
    13981 gtggtcatca cctattgcac gcaatgccag tggctgctgc gcgccgcctg gttggcgcag
    14041 gagctgctgt cgagttttac cgatgatcta tctagggttt gcctggagcc tggcacgggc
    14101 ggggtgtttc gcatcacctg cgatggcgtg cagatctggg aacgcaaggc cgatggcggc
    14161 tttcccgagg ccaaggtgct caagcagcgg ctgcgtgatc agatcgaccc agcgcgggat
    14221 ctgggccaca acgaccgctg agcgttgtta attagcgctt ggatcacttg gccatccgcg
    14281 ccgaaagaaa gatcgcgctg acaatcagca ggccaccggc gagcatgcgc agtgtcggca
    14341 cttcactgaa cagcagccag gcgaaggcga ttccgtaaat cggctccagg gcgaaaatca
    14401 ccgccgtggt gcgcgccttg agtacccgca ggctggcgac gaacaggctg tgcgccaggc
    14461 ccgtgcagaa gatcccgagc atcgccatcc atagccaatc ctgggcctgt acgctgggca
    14521 gcgtcggcca ggccagcgga ataaacacca gcagcacagt gaggttctgg tacagcgccg
    14581 cctgcaccgg atcaatgcca cgggtgctgg cgcggttgag cagcgacagc aaggagaaca
    14641 gcagaccgga cagcactgcc cagagcaggc cgctggtggc ctggctgctc aggtcgaaat
    14701 ccggggtgac cagcaccagg cccaggcaca ccagcgccac catggcgtat tcgatcggcc
    14761 gggtgcgttc acggaacagc aaaccttcga gcagcacggt aaaggccgga aaactggcaa
    14821 aaccgagggt ggcgatggcc acgccggaga tatgcacgga ctcgaaaaaa ctccaccaat
    14881 ggccaccaag cagcaggccg ccaagggcca gcaggctggt ttggcgcaag ccaaggcgga
    14941 ccaggcgcgc gttgctgatc agcttggcga acagcagcaa ggccgccacg gcgaaaacgg
    15001 cgcggccggc ggagatcatc tgcggggtgg tattggccag cttgccgaat atgcccgaca
    15061 gtccaaacag cagggcgccc agatggatgg cccacagcgc attacgttga ttcatgccag
    15121 gcgcgcaccg ttgggtagcg gtttgtcgaa tccggccagc actactttgt tatcggcgtc
    15181 atacacgccg gtcaccagca cctcggagcg cacgccggcg atgcgtttgg cggcgaaatt
    15241 gcacacgcac agcacctggc ggtcgagtag ctgctcgcaa ctgtagtggg cggtgagctg
    15301 ggcgctggag gtcttggtac ccagttcgcc aagatccact tccagcacgt aggcgggttt
    15361 tacggctttt tcattggtgc gggctgcgcg aatggtgccg acgcgcagct ccactttttc
    15421 gaaatcttgc cattcgatgt tgtgcatagg cgactcctcg attctgccgg gcagtctagg
    15481 tgcctatctg tgcccttgtc tgtctcggga gtcgcgggtt ttgtcgccag gctctcatcg
    15541 gtggacgcag gcgaattact cagtgcggcg cagttgccga ggtgtaacgc cgagatgccg
    15601 ggacagggcg gcgctaaagg cgctttgtga gctgtagccg actcgtgcgg cgatctcgcc
    15661 gatcgccagg ttgctgcctt gcagcagttg ccgcgcaagc tgcaggcggc ggctgcgtac
    15721 atagtccatc ggcgtttgcc cggtctcatc gagaaagcgt gcgtgaaaac gtgccgccga
    15781 caggccggcc aggcgcgcta gatcggccac ctgaagcggg tgcgcggcat gttggtcgat
    15841 atgcgcatct agcgccgcca gcggcagacg actggctgca tgggcgggca tctcgccatt
    15901 ggccagactg gccaagagca gggcggcgcc ttgctgggca atcaccgggt cattgatcgg
    15961 gctggcggcc agccagctga ccaactggct ctgtgcggga ttcaggctga cggcggcggg
    16021 tttttccaac aggcggcgcg tggcatccag gtgccgaccc aactgttgtt gcagccagct
    16081 gtcttcaggt acgtcgagca ccaggcagtg gctgccgctt ttgctaccac aggcgtgttg
    16141 cgcatcactg ggcaccacgg ccagggtctg gcgtttcacc tggctgccat gtccggctat
    16201 ttcgaactcc agcaggccgc tcaagccaaa caccagctgc gcgtggtcat ggctgtggct
    16261 gagcaattca tggctgtaat gacgcagcga gagcagcgag gacatggtgg gctccttgtt
    16321 cggccagcca gtttataccc tttagaggct gtcaccgaat tgccacaaca ctgtcatgca
    16381 cctttcacca cggctgcgca gactcgagca aacccagccg gaggtcgtcc atgaccagcg
    16441 cccagctcgc caagcccagc cgcaagcaac gtgtccgtac cctgtggatt tccgatgtgc
    16501 acttgggcac ccgggattgt caggccgaac acctggcggc gtttctcaaa cgctaccaaa
    16561 ccgacaagat ctacctggtc ggcgacatca tcgacggttg gaagctgcgt agcggggtgt
    16621 actggccgca gtcgcatagc aacgtgatcc gccgcctgtt gaccatgagt aaacgcggca
    16681 ccgaggtgat ctacgttacc ggtaaccatg acgaatttct gcggcgctac tccagcctga
    16741 tgctcggcaa tatccagctg gtcgacgagg ccgagcacat caccgccgat ggccgccgcc
    16801 tgctggtgat tcatggcgac cagtttgatg tgatcacccg ctatcaccgc tggctggcct
    16861 ttctcggcga ctcggcctac gagttcaccc tgaccctcaa tcgctggctg aattactggc
    16921 gtagccgtta tggctatggc tattggtcgc tgtcggccta cctcaagcac aaggtcaaga
    16981 cggcggtgaa ctttatcagc gattttgaag aagccatcgt ccacgagtgt gccaagcgcg
    17041 gcttcaacgg cgtggtctgc gggcatattc accatgcgga aatccgcccg atgggcgatg
    17101 tcgagtacat gaattgcggc gactgggtgg agtcctgcac ggcgctgatt gagcacctgg
    17161 atggcagcat cgagttgtat cgcctggccg atgatcaggc gcgtcagctg caagcagccc
    17221 cggttgtggc tgccgagccg gccctgtgag gattctgatc gtctccgacg cctggtcgcc
    17281 gcaggttaat ggtgtggtca ccagcctgca ggccttgatc agcgagctgc gtggcctagg
    17341 ccatcaggtc aagctgctgt cgccggcgga ttttcgcgcg ttgccatgcc ccagttaccc
    17401 ggaaattcca ttggtgtgga gcctctggcg ggttgggccg gcgattcgcg catttcagcc
    17461 ggactgcgtg cacctggcca ccgaaggccc gttgggctgg gcggcgcggc gctggttggt
    17521 caagcgcgat ctggcgtttt ccagcgcgat ccatacgcgt tttcccgaat atgtgcacac
    17581 gcgctggcct tggattccaa tacgttgggg ctatgccttt ctacgggcat ttcatcggcc
    17641 gagtcaggcg gtgctggtga ccaccgagcg tatgcgcgac gagtttgctg gctggggttt
    17701 taagcgcctg gtgctctggc gcaagggcgt cgatacacag ctatttcgcc catcactggc
    17761 tctgccgaac ctggatcaac cggtattcct gtatgtcggg agagttgctc cggagaagaa
    17821 tatcgaggcg tttctcgccc tcgatctgcc ggggcagaag caggtggtcg gcgatgggcc
    17881 acagcgcgag gcgttgcaga ctgcttatcc gcaggtgcgc tttctcggct atcagcatgg
    17941 ccaggcgttg gctgaggcgt ttcagaatgc cagcgtgctg gtgttcccct cgcgtaccga
    18001 tacctatggt ttggtgatgc tcgaggccct ggcctgcggt acgccagtgg cggcgtttcc
    18061 ggtagctggg ccgctggatg tgttgcaggc aggcgtcagc ggggtcatgc atgaggactt
    18121 gcgtgcggcc tgcctggagg ccttgagcct ggatcgcggg cgttgcgccg agttagccgc
    18181 gcggcaatcc tggcgagcgt cggcgctgga atttttggcg cggcagccgt tgcttgatgg
    18241 tgagctgtgc ttgcaggccc aggtgggcgc tacatgatca ccttgtcacg cagcttatcg
    18301 gctgcctgac tgagggcctg gataaccttt tccttgtcga aattctggat gccgttggtg
    18361 tccaggtaca tggaaaagcc aggtagctcg tgctcgacat agctggaggt ggcgcccagg
    18421 tcacggcgga agtccaccac ctggtagatc tgtacggggc gggtaaagcc cttgacgttg
    18481 atctggccct tgtcgcggca catgatcacg tccttgacca gcgaataggt ctcatgggag
    18541 atcaggatct caccggattc ggcggcgctt tccaggcggc tggcgaggtt cacgtcgcgg
    18601 ccgatgatgg tgtagtccat gcgcgtatca gcgccgaagt ttcccaccgt gcaataaccg
    18661 gtgttcagcc ccatgcgaat ttccagcggc ttggtgatgc cctgggcgcg ccactgctgg
    18721 cgtagcacct tcatatgctt gcgcatggcg atggccatgg acacggcatt caccgcatct
    18781 ttctttgcgc ctttgctgct ggggtcgcca aagaacacca tcacgcagtc gccgatgaat
    18841 ttgtcgatgg tgccgccgta tttcaggcag atcttcgaca tttcattgat gtaggtgttg
    18901 agcaggtcgg tcagggcttc ggcttccagt tcttcggaca gctcggtaaa gcccttgatg
    18961 tcggagaaaa acaccgtgag cttcttgcgc tgggtttcca ggcgcacgct cttcttgccg
    19021 gtgaagatcg attcccacac ctgtggtgac aggtacttgg ccagattgcg cgccaggcgt
    19081 gcggcctttt cctgttcacg ggtaatttcg ctgcgcacct gcgccaggcg caggccctgt
    19141 tgatgcacaa agtaggcggt gatgcagata tacagggtgg tgaaaaagat gctgaccagt
    19201 gccaccagcg gtggggtatt gatgtttagc ttgacctgga acagcgccgc acagaacacc
    19261 gcgccgctca gggcaatcag cgatgccagg gcaaggtgac gtacgccacc gatgaccagc
    19321 gcgctgaaca gcagaatcag cagcgacatc aggctgggaa ccaccgaaaa accaagcagg
    19381 gccagcacac cgccggcatg cagggcatca ataaacagca gactgagggt ggtgcgctgc
    19441 ggatggtcgc gtttgaaacg caggctcaga tggtgcgcca aatgcggata aagcagtgcg
    19501 tagggcacca tccagagaat gtcgtaaccg aaatgctggg tgtaagtgcc ggcggcaata
    19561 ctggcagcga tggcgatata ggccaagacg cgggagtagt actcacgcaa cggcggtgcc
    19621 ggcagtgcgg aaattcgggg ggcgcttttg acattcatcc ggtggaacaa tccctgctgc
    19681 tcgtctggca cctctgtcgg gtgcctggat agcgcagcgt ggcggctgcg caaaatagtc
    19741 agggatcata accaagccca ccgcaaccgc caagcgcggc agctgaacgg cagcgccgct
    19801 tgtgccagcg gtcattcgat tagaatttga tgcggccggt aaaggcatcc ttgagcatta
    19861 cccagtcgcc catcagcgag tagagcgggt aatcgaaggt ggcggggcgg ttcttttcaa
    19921 atacaaagtg gccgacccag gcaaagccat aaccaatcag cggtatggcc aacagccaca
    19981 accactgctg agtaattagg gcatagccca gtacacacag caccagcagg ctgccgacat
    20041 agtgcaggcg gcggcaggtg gcgttgctgt gctcctgcag gtaatagggg tagaactcgg
    20101 cgaagctgtt gtagcgttcg tgggtttcag tgctcatcgg gggcctcctc ttattctttt
    20161 gggcgccacc aggcttgcgg ctgtgacggc ttacttgcag tctaggccga ctgactcgct
    20221 gggccactga cattaggtgc cagtttagta tccttctgtc gccctttttg ctgactcaga
    20281 ataagaaaac gccatgagcg aacgtaccac ttcatccagc tgggcctcgg gcattgtcgc
    20341 ggccctggag cttggcgggg ttgattgccg cagcctgttt agtcagctgg ggctcgacta
    20401 tgcggctttg accgacgccg atgcgcgctt tgcccaggat gccatgaccc gcctctggca
    20461 tctggccgta gaggcctcgg gcaatccggc gattggcctg aatatggcga agatcctgcg
    20521 gccatcgtcc ttcaatgtgg ttggctatgc ggtgatgagc agtcgcaacc tgcaggaagg
    20581 ttttgcccgt ctggtgcgtt atcagcggat tattggcgag ggcgccgacc tcagttttca
    20641 tcccaccccg gagggctatg aaatgctcct ggcgatccat ggggaccggt tgccctcggc
    20701 ccggcagagc gccgaagcct cgttggcaca ttcattggca ttctgccgct ggctgaccgg
    20761 caagccgcta cgcccgcgtc tggtcagctt gatgggccca gcccctgctg atctggcgcc
    20821 gtatgaagaa gtgttccagt gcccgctgaa gttcaatgcc gagcactatg cgttgctgtt
    20881 cgaacgggcc gatctggatg caccgctgcc gtcggccaac gaggcgctgg cgcagctgca
    20941 tgaccgcttt gctggcgatt atctggcacg gttttccggt acgcgggtga cccatcaggt
    21001 gcgtcaggtg ttatgccgcc tgctgcccca gggcgaaccc aagcgtgaat ctgtggcgca
    21061 ggctctgctg ctatccgagc gcaccttgca gcggcgactg caggatgaag gcaccagcta
    21121 tcagcacctg ctcgacgaca cgcgccgcga gctggctggg cagtatctgg gccaggccaa
    21181 cctgaccctg ctggaaattt cctacctgtt gggcttctcc gacccgagca acttctttcg
    21241 tgccttccgc cgctggtttg acaccacgcc gggtgagtac cgtgcgcgta tgtaactgtg
    21301 cccttagtgt cgccagaatg ccggcagcat aagcaccagc acggtaagga tttccagacg
    21361 accgagcagc atgccgaagg tcagcagcca tttggcggtg tctggcaggc tggagaagtt
    21421 gcctgcgggg ccgatgatcg ggcccatgcc agggccgacg ccggagactg tgctggccgc
    21481 gccgctgagg gcggtgatcc agtccagccc acagagcgtc aggcccaggg ccagcacggc
    21541 gatggtgatg gtgaagaaga acgagaaggt cagaatcgag cggacgatgt cttcatccag
    21601 gtggtggcgg ttgtattgct gcttgatcac tgcacgcgga tggatcagct gcatcaggtt
    21661 ggccctgagc agcacgtagg ccacctggaa gcggaatatc ttcagtccgc ctgcggttga
    21721 gcccgagcag ccgccgataa accccagata gaaaaacagc atgccggcaa agccgcccca
    21781 cagcgtgtaa tcgccgaggg caaagccggt ggtggtcatg attgaggtgg tgttgaacgc
    21841 cacgtggcga atggcttcga gccagtgcag ctcggtgctc aggtaatacc aggtgccgag
    21901 cagcagccag gtgctgagca aaatgccgag aaagccgcgt acctgctggt ccctgatcaa
    21961 agccttgtag ttgccgcgca gcatcgaggc aaacagcaca aacggcacgc tgccgagaat
    22021 catcagcgcg atggtgaccc agtgcaccgc cggctcggtc catttgccaa ccgattggtc
    22081 tgaggtggaa aagccgccgg tggcgatggc cgacatggtg tggttgatcg cgtcgaacac
    22141 ccccatgcct gccgtccaca acgccaggca gccgagcagg ctgatgccca cgtaggccgc
    22201 gaccatatat ttggcgacca tatgcgagcg cggcatgatc ttttcggagc ggtccgatga
    22261 ttcggtttgg aacaggcgca taccaccgat gcgcagcatc ggcagaatcg ccacggccat
    22321 ggcgataaag ccgataccac caagccagtg cagcagcgaa cgccagatca gaatgcccgg
    22381 cgacatgctg tccagcccgg ttagcaccgt ggccccggtg gcagtgatgc ccgacatgct
    22441 ttcaaatacc gcgtcggtca cgtccagatg ctgggaaaac ataaacggca gggcagcgaa
    22501 aagccctacc agtatccagc tggacaccgt cagcaagtac atgtcgcgtg ggcgcagctg
    22561 cacatccttg ggccgacctt gggcaatcat cgcgatgccc gcgataaagg tgatcaggct
    22621 ggaccagaga aacgcgttga gttcatcagg ccgcgtaaac agtacgagtg tcagcatggg
    22681 cacggccatg ctgatcgcca gggtcagaag aaagatgcca ttgataaacg cgatgatccg
    22741 taaggtcggc caagccatct aagcgggttc cgttccagta atagcaagag tctaggttat
    22801 cgggcttgtt cgcttgtcgc acagccgatt tcactcaatg tcgccagaat gcccgagtga
    22861 ctagcaccag tacggtcaaa atttccaggc gaccgagcag catgccgatg gtgagcagcc
    22921 acttggcggc atccggcagg ctggagaagt tgcccgccgg gccgatgatc ggacccaagc
    22981 cggggccgac gttacacact gcggtagccg cgccggtcag cgcggttacc cagtccaggc
    23041 caagcagagt aaggcccagg gcaatcacac cgatggtgat ggtgaagaaa aacgaaaagg
    23101 tgatcagcga gcggacaatc tcttcatcga ggttgtggtt gttgtactgc tgcttgatta
    23161 ctgcgcgcgg gtgcaccagc tgctgcaggt tggccttgag cagcacgtaa gccacctgaa
    23221 agcgaaagat cttcagcccg ccggaagtgg agcccgagca gccgccgaca aaggtcaggt
    23281 agaagaacag cagcacggcg aaactgcccc aggtggtgta atcgcccagg gcatagccgg
    23341 tggtggtgac caccgaggtg acattcactg cgacgatgcg aaacgcatcc agccagctgt
    23401 tatccgaatg cagccacagc caggtgccga acaccagcca ggtgaccaac aaaaagccga
    23461 gaaagccgcg tacctgatgg tccttgaaca gcgccttgcg gttgccgcgc agggtggcga
    23521 catacagggt aaagggcagg ccgccgaaga tcatcaatac caccgcgacc cagtgcacgg
    23581 ccggctggct ccagttggct agggatgagt ccgaggtcga gtagccgccg gtggagattg
    23641 aggtcatcgc atgattgagc gcatcgaagg gcgtcatgcc cgccagccaa aagccgagaa
    23701 agcccgtcag agtcaggctc aggtagataa acaggatgta tttggccgcc atgtgcgagc
    23761 gcggcatgac tttctcgccc cagtcggagg actcggtctg gaacaggcgc atgccgccga
    23821 cccgcagcag cggcagaatc gccaccgcca tgccgataaa gccgataccg ccaagccatt
    23881 gcagcatcga gcgccagatc agcaggccag gggaggcgtg gtccagtcct gcaagcacgg
    23941 ttgaacccgt ggtggtgacg ccggacatgg tctcgaagaa ggcatcggtg tagctgatgt
    24001 ggtggatcag caccatcggc agggcggcga agatgcacac caccagccag ctgccggtgg
    24061 tcagcaggta catgtcgcgc gggcgcagct gcgcctcttt tggtcggcca gggatgacca
    24121 tggcgatgcc cgccgcggcg gttaccaggc tggaccagag aaaggcgttg aggtcgtctg
    24181 tgcgcgaata cagcagcagc gtcagcatgg ggatcaacat gctgacggcc agggtgatca
    24241 ggaagatgcc gagaataaaa gcaatgatgc gcagtgtcgg caatgacatg gattcggctc
    24301 aggctctgtg gggcaaaggg gcgttattct acgcgcgctg cagccgctgt acagccgcca
    24361 taatgcgcgc tttacaagcg cttggcgctg catagaatag ccgccaggtg ccggcccttg
    24421 ccggtttctg agcccgccaa atcatgtccg atagcaatcc ttgtcttagc tgtggcgcct
    24481 gctgcgcgca ttttcgtgtg tcatttttct ggggtgagtg ccaatcggcg ggtggtgtgg
    24541 tgcccgatga tcgggtggtg ctgatcaccc cgcatcgcgt cgccatgcgt ggcacggagc
    24601 acaagcccgt gcgctgcacg gccctgctcg gtgaagtcgg ccagggcgtg cgctgcacgc
    24661 tatatgaaca gcgctccagc acctgccgcg agttcgaggc ctcctgggtc aatggcgagc
    24721 acaacccgca ttgcgatgcg gcgcgcggcg cacacggttt gccgcccata atgccgccgg
    24781 tgttgcccag tatttcgcct gaacgggtgg cttgagaaac tctacgtatt gcctgtctgc
    24841 tgcacccagg cactagaatg gccacctgtt tcaattgccg aggtggctga tggacgctct
    24901 tgatgcgttg ctaaaccgtg tttccgcgcc gcgcctgatt gcgccggccc ctgatgctgc
    24961 gcagcgcgaa ctgttatttc gcgccgccct gcgtgcgcct gaccatggcc agctgcggcc
    25021 ctggcgtttc ctaaccgttg aaggcgaagc gctgacgcag ttgggcgcgc tgtttgccag
    25081 cgcgttgccc gccgatgcca gccccgaagc ggtgaccaag gcgcgcgcca tgccgctgcg
    25141 ggcgcccttg ctggtggtgg tgattgcccg cgtgcaggcc agcttgaaag tgccagcgca
    25201 ggagcaggtg ctggccgccg gctgcgccgc ccatggcatt cttctggctg cccatgcgca
    25261 aggtattggt gcggtctggc gtaccggtga tatggcttac aacccgcagg tcgctgccgg
    25321 tctggggctg agcgcagatg agcaggttat tgcctacctc tacctcggca ccccagagcg
    25381 cgaactgcgc gcggtgccgc aggtacaggt cggggatttt gttaaggcct ggcctgcggt
    25441 ggagtgagtg gcaggcgcag agtggcgata aagccgcctt gcgcatgatt gctcagttcc
    25501 aactggccgc catgccgctc ggcggcgcgg cgggcaatgg ccaagcccag accgtgaccg
    25561 ctgctggtct ggccaggggc gcggtagaag ggttcgccaa ggcgggtcag cagctcaggt
    25621 gcaacgccag ggccgtgatc acgcacgctc aggcatagct gctcagcgtt tttgctggcc
    25681 gtcagctcga tagcctggcc aaccgggttg aaacgcaggg cgttacgcag caggttatcc
    25741 atcgcccgtt gcagcatgtc cagccagccg ttgagctgca ggtcgctttc cagctgaatc
    25801 ttgatctgtt gctccggcgc gtccaggtgc gcgtcttcct gcaatctgtg cagcagctcg
    25861 tctacgttga ttggctgcgc cgcacccggt tcggcgtcga ggcgggcgag ggcgaggatt
    25921 tcactgatca gcgcttccag gcggtcgcac tcctgactca ggcgcggcca gtatttttcc
    25981 cgttcggcag catcggcgcg ctcggtcagc gccaaggcaa tacgcagacg cgccagtggt
    26041 gagcgcagct catgggacac gtcgcgcaac agctgacgtt gactgctgat caagccttgt
    26101 acgcgttcgc ccatgcggtt gaagtcggcc gccagcacgc cgagttcatc acgccgttcg
    26161 gccagtttgg ccaggctgtt ctgctggtag gcggtttgcc ccagatcatg cacggcgccg
    26221 cgcaggcgat tcagcgggcg ggtgatggac agcgtcagcc agaggctgaa cagagtcagc
    26281 accagcatgg cgatagccag tgcgctcagc ggccagagca ggctttcgcg ctgccacgcg
    26341 gcgacttcgg ggttcgggat gcggtagata aacaggtagg tttcgccgct atgcgggctg
    26401 gtgtagtcga cactcagacg gcgccagggc aggcgtttct cattgctgtg gcgggcttca
    26461 aaggcagccg cgcgaggcgg gaaggtgcct ttgaccaccg cttggccgct gtcgtcgagc
    26521 acctgtacgt cgatgcggaa cttgcgtttg cgttgttcga gtagcgcttg ggcggagctt
    26581 gggcctttct tctcgtagcg ctgggtccac ttttcggcga ggtcatccat gccggggtgc
    26641 tggctgagca accaggcgtc ctgattgagc atgcgcccaa gcagcataga cagcccagca
    26701 accagggcaa tagccagcca gaagctggca aagatgcgcc agaacagtga ccgcacggtg
    26761 gctccttagt agtaatggct gtggtggaaa tgcttacgcc catcaggctt ttcagcctga
    26821 tgggcgtaat ggcaacagtg cctgggttag ttctttttcg cctgctcggc ctgccaggct
    26881 ttgaatgcct ggcgctctgc gcggcgctct tgcatctttt tctggtgctc gtcgaaggct
    26941 ttctgctgct cgggcttgag cagggcgcgg atggcgctgt gctgtttgtc cttggcggcc
    27001 tccagttcgt cctgcatggc tttctgttca gcagctggca gcttgtccag gtagcgcttg
    27061 gtgatctcat ggcggccttg catctgctcg cccatcagct tgcggatttc gcggtgctgc
    27121 tctttgctca ggtcgagctc tttgaacatg cgggagccat gttcaccatg atggccgccg
    27181 ccgtgccgcg ggccgccgtc aggcatggcc atggccagag tcggcagggt cagggcgagc
    27241 agtactgcgg taagggtctt gcgcatggtg tgtctccttg tctgaaaccg gtaaacaccg
    27301 gatgagccca gtctagggcg gtcaaggtca agggccgtca gggcaaggta aagcttaggt
    27361 aaagagtgcg gtgtggctga tttacacagc gactgctcaa acgctgtagt agtagccgcg
    27421 gctacgcaga gcaagaatgc gctggcggcc atcggcatgc gggccgagct ttttgcgcag
    27481 gttgctgacg tgcatatcca ggctgcggtc atacagggtc agtttgcggc cgagggccag
    27541 ttgcgccagt tcctgtttat ccactggctc acccggatgt tgcagcaggg cttcgaggat
    27601 gcggctttcc gaaagtgtca acagcacttc ctgttcgccc agggtcacgg tgccgcgagc
    27661 ctggctgtaa tgcaggtcgc cgagatcgag cgggctgcta ggcgtggccg gcaggctacg
    27721 gcgcaagacg gcacgcaagc gcgcggttag ctcgcgaggg tcgcagggtt tggccaggta
    27781 gtcgtcggcg cccagttcca ggccgagaat ccggtccagc ggttcgccac gggctgaaag
    27841 catcagcacc ggcagttcgg ggtggtcgct gcgcaactgc ttgagcagct ccaggccgct
    27901 gccgtcgggc agcatcacgt ccagcaccac tgcatcaggg ctgtgtttgg caagggcgtt
    27961 gcgggcactg tggccgtcat ggcaggcgct aacctggaaa ccctcctggg tcagccagct
    28021 ggtcagcagt tcgcagagtt cgacgtcgtc gtcgattaac agcagatcac tcatggctta
    28081 gttgagccac tcgctgcgtg agcggcgacc acggcgcagc agggcaccga caatcacccc
    28141 gagcaaaccg gcgccggcgc ctgtggcaaa ccacatctgc tgttcgctga tcagcggttg
    28201 cggggcgctg gcctgggctt ttttcagctg cagcttgagg cgctggtttt cctgacgcag
    28261 gcgctgcagt tgcacggtgt cacgttcgtt gctgctgttc tgcagttcag tgctgagggc
    28321 ttcgcgctgt tcttcgctga gcgccaggcg ctgctccagt tcatcgatct gcgtctgcac
    28381 cgaagtggct tctgccaatg gctgcggcgg gctttcctcg gcctgggtga cggccggcaa
    28441 gcacaaggtc aagagcagga acagcgggcc tggacgcatc ggaactccta tctttgtaat
    28501 tgttgttatg ggttattcag cgagggattg gctcaaggca agaccagttt gaagggtttt
    28561 accagcaccg aggcatacac accgacggcg cggtaggggt cggcttcggc ccattgctgt
    28621 gcggcttgca gcgattcgaa ctcggcgacg atcaggctgc cggaaaaacc cgctggacct
    28681 ggatcattgc tgtcgacggc cgggtgtggg ccggcgagga tcaaacggcc ttcagttttc
    28741 agctgctcca ggcgcgcaag gtgcgccggg cgagcactca ggcggttttc cagggagttt
    28801 tccacatcgg tggcaatgat cgcgtagagc atctcaatcc ttaatacggt ggttagcgcg
    28861 ggccagcgcc aggggagaaa agcagcctga tcaggcaaag tcgaggaaat attggcgcaa
    28921 gccagcaaga atccacatct ggcggcataa taacaaacat aaatgactcg gaaagcgccc
    28981 gttatggatg ttgatttgca ttgccacagc accgcttcag atggcgccct tgcgccggct
    29041 gtggtggtgg cgcgggcgtt tgagcgcggt gtgcgtttgc tggcgttgac cgatcacgac
    29101 accctcgatg gcctcgacga agcgggcgct gcagcgcaag cgctgggcat gcaactggtc
    29161 aacggcattg agctgtcctg cacctggggc ggtgcgacta ttcatgtgtt gggctatgcc
    29221 ttcaaccgcg acgcgccggc tttgcagcag gcgattgccg aactgcatga agggcgctgg
    29281 ttgcgcgccg cagaaatcag caaacgtctg gaagccaaag gcatgccggg tgcgctggaa
    29341 ggcgcgcggg ccatgcagca ggagttgggc gacagcggca atgctccggc acggccgcac
    29401 tttgctgact ttctggtgcg cagtggttac gtcaaggatc gcgccgaagc gtttcgcaag
    29461 tggctgggct cgggcaagct gggggatgtc aaacagcact ggccggagct ggctcaagca
    29521 gtcgataccc tgcgccgcgc caatgcctgg gtcagtctgg cgcatccctg gcagtacgac
    29581 tttactcgga gtaaacgacg caagttgatc gccgactttg tcggcgccgg cggccattcg
    29641 ctggaagtgg tcaacgggat gcagccggcc gaacaggttg gcagtttggc gattttggcg
    29701 cgggagttcg gcatgtttgt caccgcaggc agtgatttcc atgcgcccgg tcagtggtct
    29761 gagctgggcg tctatcgccc ggtgcccgag gatctaccgc ccctgtgggc acggtttcaa
    29821 catgtccagc agcctactgc aatttgacca gggtccaagt gtgagccaat tcttccagat
    29881 tcatccggaa aacccgcaag cgcgcctgat caaacaggcg gtggaaatca tccgcaacgg
    29941 tggggtggtg atctatccga ccgattcctc ctacgccgtg ggttgtcata ttggtgacaa
    30001 gaacgcggta gagcgcatcc gccgcctgcg ccaactggat gacaagcaca acttcaccct
    30061 ggtatgccgc gatctgtcgc agctcagcac ctttgctaag gtcgataccg ccgccttccg
    30121 cctgctcaag agtcataccc cggggcctta cacctttatc ctcaacgcca cccgtgaagt
    30181 gccgcgcatg ctgctgcatg ccaagcgccg cactatcggc ctgcgcgtgc ccaaccatcc
    30241 gattgccatg gccttgctgg ccgagctgga tgagccgttg atgagcgtga ccctgattct
    30301 gccgggtgaa acgctgccga tgagcgaccc ttacgagatg cgccagatcc ttgagcatca
    30361 ggtcgatctg attatcgatg gcggtttcgg cgggctggaa gcctccaccg tggtcagtct
    30421 gcttgacgat gagcctgagg tgtttcgagt gggttgcggt gatccgtcgc cttttacgaa
    30481 tgacatctga tcctggctat aatcgaactc ctttgagctt tcaggtaatc cgttttgagt
    30541 cttgttgacg ctgaacgccg cgtgctgtct ggcatgcgcc cgagtggtct gctgcacctg
    30601 gggcattacc agggggtgct gaagaactgg gtgaagctgc aacacaccta tgaatgcttc
    30661 ttctgcatcg tcgatctaca cgcgctgacc actgattacg atgacgtcgg ccccttgtct
    30721 cagcgcgtca tggacatggc cgtggactgg ctcgcggccg gtgtcagccc cagctcggcg
    30781 acgctgttta tccagtcaca agtccctgaa cacgccgaac tgcacctgct gctgtcgatg
    30841 atctgcccgc tcagttggct ggagcgggtg ccgtcctata aggagcagca ggagcagtta
    30901 cacggcaagg acctgagcac cttcggcttt ctcggttacc ccttgctgca ggccgcggac
    30961 attctgctgt accgcgccgg cgtggtgccg gtaggcagtg accagctggc gcatatcgag
    31021 tttgcccgcg atgtggcgcg gcgcttcaac catttgtatg gccgcgagcc aggctttgag
    31081 gaaaaggccg aggcggcgat catcaagctg ggcaagaaaa ccgcaacgct ctacaacaat
    31141 ctgcgtaagg cctatcagca gcagggcgac gccgaggcgc tggaaaccgc gcgggcgctg
    31201 cttaaggatc aacagagcat caccctgggt gataaagagc gcctttatgg ttacctggag
    31261 ggcggcggca agattctgtt gatcgagccg caggccatgc ttggcgagtc gcccaagttg
    31321 cttgggctcg atggcggcaa gatgtccaaa tccaacaaca acgcgatttt cctgcgtgac
    31381 agcggtgcgg cgatcgagga gaagatccgc cgtatgccga ccgatccggc gcgggtgcat
    31441 cgggatgacc ccggtagccc ggcgaattgc ccggtgtggc cgctgcatca gctgtattcc
    31501 agcaacgaga cctgcgactg ggcgcagcaa ggctgtcgca gcgccggcat cggctgtctg
    31561 gagtgcaagg cgcctttggt cgaggcgctg cagcaggagt tgcaaccgtt gcaggcgcgc
    31621 gccgcggatt atcaggacaa ccctgatctg gtccggcaga ttcttgccga tggcgctgac
    31681 cgcgcgcgct ccgaggcgcg ggaaacccta agtgaagtgc gcctggccct tggcctgaat
    31741 tatcgctgaa cctaacgctg aatctaattt cgagaatgac catgtccagc gtgccagccc
    31801 cggaacatcc cgagagccat ccccaggacc aggccaatgc ccagcaagag ctgccatttg
    31861 ccctggtata tggccaggcg gtcaccgaaa tgccgctgga cctgtatatc ccgccggatg
    31921 ccctggaggt gtttcttgaa gccttcgaag gcccgctgga cctgctgctc tacctgattc
    31981 gcaaacagaa catcgatgtg ctggatatcc cggtggcgga aatcacccgc cagtacatgg
    32041 gttatgtcga gctgatgcag tcggtgcgcc tggagttggc ggccgagtat ctggtgatgg
    32101 cggccatgct cgccgagatc aagtcgcgca tgttgttgcc gcgctccagt gaggtcgaag
    32161 ccgaggaaga cgacccgcgc gccgagctga tccgccgcct gcaggaatat gagcgttaca
    32221 aggctgctgc agaaggtatc gacggcctta gccgggtcgg ccgtgacgtc accgtgccgc
    32281 gtctggacgc gcccgaagcg cgggcgcgca agctgctgcc ggatgtcagc ctagaagaag
    32341 tgctgctgtc gatggccgaa gtgctgcgcc gcgccgatat gtttgaaagc caccaggtca
    32401 cccgtgaagc gctgtcgacc cgcgagcgca tgagcgaagt gctcgaacgc ctcaagggcg
    32461 gcgcgtttgt gccctttgtc agcctgttca gcgccgaaga gggcaagctc ggtgtggtgg
    32521 tgacctttat ggcagtgctc gagttgatca aggaatccct ggttgaactg gtgcaaaatg
    32581 aggcttttgg gccaatccat gtgcgtgcgc gagctgaata agcatgaacc ttaacgaacc
    32641 tcgtgagctg gccagcctgc tcgaagcctt tctgctggca tccggcaaac cgcaatcgct
    32701 ggagcgtctg tacgaactct tcgaggaggc cgaacggccg gagccagcgg tcttcaagaa
    32761 ggccctgacg cacctgggca agtcctgcga agggcgcgcc ttcgaactta gcgaggtggc
    32821 gtccggttat cgcctgcaag tacgcgagaa gtttgcgcct tgggtgggtc gcctatggga
    32881 agagcgtccg cagcgttatt cgcgcgccat gctggaaacc cttgcgctga ttgcctaccg
    32941 ccagccgatt acccgtggtg agatcgagga tgtacgcggt gtggcggtca acagccatat
    33001 cgtcaaaacc ctgctggagc gcgagtggat tcgcgtggtc ggttaccgcg atgtgccggg
    33061 taaaccggcg atgtttgcca ccaccaaggc ttttctcgat cacttcaacc tgaaaagcct
    33121 cgacgaatta ccgcccctgg ccgccctgcg cgagctggaa ccggagccgg cgcttgcctt
    33181 cgaggatgat ctggaggcgc cgcaaagcct gcaggcgcgt gccgacctgg ccctggccga
    33241 tgagcctgcg gtggaagtgg gcgaggaaac cagcttcagc agcctgctgg ctgaactcga
    33301 ctcgatggaa cagggtctga agaccgactt cgacgatctg cccgaacagg acgatgagct
    33361 tgacgatgcc gaagagcctc acgaaaccct ggataaagag caactgcctg actgaggttc
    33421 gtaggatggg gggccaccga tcaacggtag cccggataag ccctggcgca atccgggatg
    33481 gcagctcccc ggattgcatc cgggctacgg tgatgggcta gtaagtaggc gccgattgtc
    33541 gcaaaggctg acgactgctc gtctgccagc tagcctcaag tcgtgctaat cggccggctt
    33601 tcgcgtatgc tccgcgcccc ttcgacgact cgagtcgtcc aatcactaaa gaaaacaccg
    33661 ggaggtgccc agatgagtga agaaatcgaa gaatacagcc ccgcaggcga aaaactgcag
    33721 aaagtcatgg cgcgtatggg cctggcttca cgccgtgaaa tcgaagcctg gattaccgct
    33781 ggccgcgtca aggtcaacgg cgcagtcgcc agcctcggcg tgcgcgtcga cctgcacgat
    33841 gcgattgccg tcgatggtaa ggtgattcgc cgcgaagaag ccgccgaaag cgtgcgccgc
    33901 gtaatcatct acaacaaacc cgaaggcgag atctgcaccc gtgacgaccc ggaaggccgt
    33961 ccgaccgtgt ttgaccgtct gccacggccg aaagatggcc gctggatcaa tatcggtcgc
    34021 ctcgacatca acaccaccgg tttgctgatg ttcaccactg acggtgagct ggccaaccgc
    34081 ctgatgcacc cgtccttcca gatggaccgc gagtacgccg tgcgtgtgcg cggcgaagtc
    34141 gacgaagaga tgatcgaacg cctgaaagct ggcgtgatgc tcgaagacgg cccggccaag
    34201 ttcaccgata tcaaggtcgc gccgggtggc gtgggcttca accactggta tcactgcgtg
    34261 gtgatggaag gccgtaaccg cgaagtgcgc cggttgtggg aatcccaggg cctggtggtc
    34321 agccgtctga agcgtgtgcg tttcggcccg gtgtttatta cctcggacct gaccatgggc
    34381 cgctggcgcg aaatgagcca gcgcgaagtg gatatcctca gcgaagaagt cggcctcaag
    34441 ccggtagcct tgccggatat gaaggaaaaa gcccgcgaca agatcgaccg tctgcagcgc
    34501 aaatcggcca agccggtggg tcgtgccgca cgtccagagc ggaccctgcg tccggcccat
    34561 ggcggcggcc cggcagcggc tggcgatcag ggtcgttcgc gtcctgcgcg tggtgaaggt
    34621 gctgagcgtg gcgagcgtcc ggtacgtaac ccacgtacgc cgcgtgttga tcagagcaag
    34681 ggcacgccgg ttgccgagcg cccgagtgat gtcaacaaga agcgtccggc taagccgggt
    34741 gcgcagcgct caggcgtcga gctgagcgag cgtccatcgc gcaagccagc cggtgcgccg
    34801 gtcaagcgcc gcagcccgcc gagccgtgaa ggtcagcgcc caggttttgg tcgcggtcct
    34861 aagccggagt aatccattac ggtggatatg aaaaaggcga ccctcgggtc gcctttttat
    34921 tgtcgggcgg tttacccggc catcgacaag cgattacggc catcgcgctt ggatacgtac
    34981 agcgcgttgt cggcgcggcg cagcaggctt tccaccgatt cacctggcag cagggtggcg
    35041 cagccgaggc tgaccgacag ttcgatggcg cggctctcga ccaagtactg gatgcccagg
    35101 accgccatgc gcagacgctc accaaccatc gaggcggctt cgcggttggt gttggagagc
    35161 agcaccataa attcttcacc gccgaagcgg aacaccatgt cgacgttacg caggctgtct
    35221 ttcagctcgg tggcgacggc tttcagtgcc tggtcaccga tcaggtggcc gtggttatcg
    35281 ttgatgcgct tgaagtggtc gatatccacc atcagcaccg acagcggttg cagcgtgcgc
    35341 cgggcaatgt ccacttcgcg cttgagcgtt tgctgcatgg caatgcggtt gccggtatcg
    35401 gtcagcgggt cgcgcagtgc gctctgcact gccgaacggt aaagcagggc gttgcgcagt
    35461 ggaaacagca ggctggcgag cagcgattcg agctggccca gctcatcctc gctgaagcgc
    35521 tggttgcggc gaaaggtcaa ctcaccgagg tattcgccat cgtggttcag gcggtagccg
    35581 gccgagtgat tgccgcgctc accgaggtcc aggcgcaggt cgcaggacgc caggtggtag
    35641 ctcagggcgc tgagtggtac caggcgctgc acttcagtga agaacagctc gagaatgcgt
    35701 tcggcttcca aggaggtctg caggcgcagg ctcaattgct ggcgcagctg cgccaggctg
    35761 accggcttca ggctcttctg caggcgaccg gcatagccga gacgttgcag ctttgcagcg
    35821 tcgaaatcga tggtgttgga ctgattgtgc ggggccataa agccttacct cttacgcgat
    35881 acgcgacgac tggaagaggt agagcgaatt tcatgccaag cgcggaagtg tgtgaaaaat
    35941 gcttttattt catgtgctta gatttaagtg gcaaaggctg gtggcaagtc tttgccggtt
    36001 aactggcggg gctgctggca atttgatgcc aggtgaatcg gctgccgccg gaaaaccggc
    36061 ggcagccgtt atggcttatt gcgcgtcaaa tgcctggccg ttgacacccg tgctatccgg
    36121 ccccatcagg tacagataga ccggcatgat cgcctcaggc aacgggttgt tctgcgggtt
    36181 ctcccctgga taggcctggg cgcgcatgtc ggtgcgggta gcgcctgggt tgacgctgtt
    36241 ggcgcgcacg ctggcgatgc cgtcgacttc gtcggcgagc acctgcatca ggccctcggt
    36301 ggcgaacttc gacactgcat aagcacccca gtaagcgcgg cccttgcggc cgacactgct
    36361 ggaggtaaac accaccgagg cgtccgtcga gagtttgagc agcggcagca gagtgctggt
    36421 gagcataaac atggcgttaa cgttgacctg cataacgcgc atgaagttgt cgccggacag
    36481 ctgctccagc ggtgtgcgtg ggccgatgat cgaggcgttg tgaagcaggc cgtcgagttt
    36541 gccgaactcg gtttcgatca tcgccgccag ttcgtcgtac tgatgcggca gggcggtttc
    36601 caggttgaac gggatgactg cggcctgcgg gtggccggcg gcttcgatgg cgtcatacac
    36661 gcggctgagg ttttcttcgg tcttgcccag cagcagtacg gttgcgccat gggctgcaaa
    36721 ggtcttggcg gcggttgcgc caatgccacg gccggccccg gtcaccagaa tcacgcggcc
    36781 cttgagcagg tcggggcggg cggaatagtc atgcatgggt aaagcctcat taattcaaaa
    36841 gcgggtggcg tcggtgcgcg cccggcaggc aattttcagc agctgcacag ggctcgttcg
    36901 agtacggtgc gcagttccag cggatggtcg accaccacat cggcgcccca gttgcgtggg
    36961 ttgtcctgcg gatggatata gccgtaggtg acggcggcgg tcttgcagcc ggcggcgcgg
    37021 ccggcttcga tatcgcgctc gtcatcgccg ataaacaggg tggcggcggg ctccacgccc
    37081 atcttcgcgc aggccagcag gatcatctcc ggatcgggct tgctgcggct gacgtgatct
    37141 gggcagacca acaccgccga gcgctgcgcc aggccgagtt gctgcatgat cggctcggca
    37201 tagcgcaccg gcttgttggt cgccacaccc cagatcagct tggcctgttc gatgtcatcg
    37261 agcagctcat tcatgccatc gaatggccgg gtcagcaccg cgcagtggct ctgatagcgt
    37321 tcgagaaact ccaggcgcaa ggcttcgaag cctacggcct cggggtccat ggcgaagttg
    37381 gcggcgacca tggcgcgcgc accgccggag acctgatcac gaatcagctt gtcggcaata
    37441 ggcggcaggc cgcgctcggc gcgcatgacc tggctgatgg cgacaaagtc cggcgcgctg
    37501 tcgagcaggg tgccgtccat atcgaataac actgcgcgta aacgcatctg ccttactcct
    37561 cgcgcagagt ctggatcata tagttgacgt cgacatcgga ttccagcttg tagcgtttgc
    37621 tcagcggatt gtaggtcaga ccgatgatgt ccttgacctc aaggccagct tcgcggctcc
    37681 aggcgccgag ctcggacgga cggataaatt tcttgaagtc atgggtgccg cgcggcagca
    37741 ggcgcatcag gtattccgca ccgatgatgg cgaacaggta cgccttgggg ttgcggttga
    37801 tggtggagaa gaacacctga ccgccaggct tgaccatgcg gtagcacgcg cggatcaccg
    37861 acgccgggtc gggcacgtgc tcaagcattt ccaggcaggt gaccacgtcg aactgttcag
    37921 gcatttcctc agccagggct tcggcggtga tttgccggta ctcgacgctt acgcccgact
    37981 ccagctggtg cagttgggcc acggccagcg gtgcttcgcc catgtcgata ccggtgacgc
    38041 tggcgccgcg ctgagccatg gattcgctga ggatgccgcc gccgcagcca acgtcgagca
    38101 cttttttgcc ggccagaccg acgcgctcgt cgatccagtt gacgcgcagt gggttgatgt
    38161 catgcagggg cttgaattcg ctttcgcggt cccaccagcg atgggcgagg gcttcgaatt
    38221 tggcgatttc ggcgtggtcg acgttgctca tataaatccc tgaatcaggc tgcggcaaga
    38281 atgggaacgc aaggtgctca tgttgccagt ttcgctgggt ggattggacg cctgatgtgc
    38341 gtccaatgta tgtcagtttg tcgcagtgct tgctgtcaat cggctttggg ggttttagcc
    38401 gcgacttgcc acataaggtg ttggttatcg cggctaaagc cccacgctct gtactcttgc
    38461 ctacatagcc ggcccgcaac ccgttagcga gatcaatctg atgaccgctg ctgggttagg
    38521 tgacgcacag attgcgcagt tgctcaaatc cggctttgcg ccacacaccc gccctgcaaa
    38581 agctgcgcgt gccggctctc tgattacatt gcagttagcg cggatatagc ggcggcaggg
    38641 cgctggtgtc cgggttggtg gtgctctcaa ggtcttctac ggcgggcagg ctgcgaatcg
    38701 ccagccacag attgcggccc tgccagtgct ggccgctctc gctgtacagc gcaccattca
    38761 ggccatcgag ggcgtcggac agtggggtaa agcgcgcggc catatcggca agggtttccg
    38821 gctgctggcg ggcccaggcg tccagcgcat ggcgagtggc ctgcgagtcg ttggctaggc
    38881 agctgcgctt gaggtcatcc agcagattgc gctggctggg gccagtctgt tggctgggca
    38941 atacgggcgg ctggcgacgc gcgcgccacc acaaggtaaa gccgagcagg gtggtcaggc
    39001 tcagcagtgc gctgctcagc tgccagggcc agagttgtac gttggcggcc tcgctgactg
    39061 cagggcgtgt tgcctcaacc ggcagggttt ccaggttcgg gttggcagct acctgcaggt
    39121 tacgcgccgg caagctgctg cgttcgagtt gatcggtcgt ggtgttccac cacagcactt
    39181 cgattgccgg caaggcaatc acgccctcac gattggctac cagcgcttcg cgctcttcac
    39241 ggctggccac taagccgttg tcgctggcct gattggccag ttgcggctga tctgggtaac
    39301 ggcgcaggcc ggctacctgg gtggctggca agggcggcag ttgtgcgctg gcaaggcctt
    39361 cgaccttgag cagcaggctg cgggtcagcg agtcgccgac ccgggccgtc tccggctgcg
    39421 ggctccagac ttcactcagg ctcagcgcgc gcgccggcag ccagggcgcg tcggctgggt
    39481 agctggcggg cttggctttt actgtcagtg ggatttccgg tgacctgacc cgtaccacct
    39541 tgcctgggcg cgggccgaac gggttgtagg cattgctggc ggagcgatca accgcagtgg
    39601 cggtaaaggc ctgggagggg attagcagct cgccactttc ctgggggaag atcgcgtaac
    39661 gtagctcgat cacgccatgg cgcacgccgt tgatgtcttt ctcgtaggtg cgcggctcgc
    39721 cgagcgcttc aacacgcgcc tggggcatgt ccagcggggt caggctgctg tcgtcataaa
    39781 gcgaaacgga gtgatagata cgcagcgtca gtacggtttg cgcctgcacg tagacgcttt
    39841 cctgatccag gctggcgtcg ataaataccg gggccaggcg actgtcgtcg gcgctggaga
    39901 cctgctgtac gtgcagggtg atgggttcgc tttgcagctg gccgaggctt agtggcggta
    39961 tcaccacata gccgctgtgc ttgggttgca gggtgacgat ccagcgggtg ctggcctggg
    40021 cgccattgtt gccgctggtc aggcggttga cctgacgggt gccgagcacc tcgaacagct
    40081 cattgagcgg ctgcaggtcg ggcttgccga acagcgtggc gtcatccgac tccagggtca
    40141 gctcaacgct ctcgccggca tccaggcggg tgcggtcgac gctggcggtc aggctttgcg
    40201 cactgacgtt cagggccagc aggccaagca gaagggtaca gagcagtcgg ctcattgcag
    40261 ttgttcctgg cgctgttgtt gttccagcct gaatttacgc cgtagcagct caccgggatc
    40321 gtcggggatc tgccgcagcc attgttccaa tgcctggcgc cgctcgccgt cgagggctgg
    40381 ctcggcgctg gcgatctgtt cttcgccgct atcgctgtcg tcttcttctg gttgtttcgg
    40441 cggtgtatcc gcgtcttcgt cggagccggg ttgtgcaggt ttttcgccct tatcctcagg
    40501 ctcggacggc ttggccggcg attgctcggc ttgctgcttt gcgtctggat cggactggcc
    40561 tgcctgtggc tgactgctgg gctgattctg ctgctcgttg ggttcttgcg ggttgtcggc
    40621 ttgattctgt tgctgctggt tctggcgcag cagctcttcg accagggctt tgtttttttg
    40681 cgcctgagcc aagtcgggtt gcagctccag cgcctgggtg taggcgtcca cggcggcttc
    40741 cagttcattg ctgcgcgcca gggcgttgcc acggttgtag tggtcgctgg cggtttcgcc
    40801 attggcgaag cgttcggcag cagcggcgta atcgccggcc tggtagagcg cctggccttg
    40861 ccaacgcgga tcgtggaaac gttgcgcggc tgccgctggt tgctgcgcct caagcaggcg
    40921 ctggccttgc tggtcggcgc gcagccagag gtcgtcgaag ctgaaagcgt agctgttctg
    40981 cggcaggctc agcagcaaca gcggcaaaca cagcagccag cctcggcgtg cggcgcaagc
    41041 ggcgagtaac agtaagggca gaagcagcca gtggctctgg tcagcccagg cttgcaggcg
    41101 ggtcagctcg ccatcgcggc gcagctgttg cggcgcatcg agcaggccga ggttgcgcag
    41161 gtcgcgttca tccagcgtca cgccctgata caggccgccc agttcactgg caaagcgctt
    41221 gaggccgttg ctgtccaggc gcggcagcag gatgctgccc tgggcatcct tgaggaacga
    41281 gccgtcctcc tgaatgatcg gcgcgccctg cgtgctgcca ataccgagaa tgcgcaggcg
    41341 ctcgccacgg cggccgaggg cctgggtgat cgcctggcgt tgggtttcat cgaggctgct
    41401 ggtcagcaac agcaggcggc cattgccctg ggcgccctgt tcgagcagtt gcagagcttt
    41461 ggccacagcc aggtcagcgc gcttgccagg ctccggcatg atttcggggc tcagcacatc
    41521 cagcagattg cggctggtcg ccaggtcgtc ggacagcggc accagggtat gtgcgctgcc
    41581 ggcatacacg acgatggcgg tttgcgcatc ctggcgcgct tccagcaggt cgagcagctt
    41641 gcgcttggcc tgggccaggc gattgggcga ggcgtcgctg gcgagcatct ctggggttag
    41701 ttcgagcaat accaccagcg ggtcggccgg cttggggttc tgctgctcga tgcgttgcca
    41761 gctcgggcca agcagcgcca acagtgccag cagccaggcc aggccgagtg ccagccaggg
    41821 cagacgactg ttgcgcccgc cggtggcagt cagtagcgcg gcctgaaagg cagctgggag
    41881 cagcgcttgc cagcggccgg tgcgtttttc ccggtgccag agttgccaga gcaggatgcc
    41941 aagtagcgga atcagcagca gccagaaggg ccgttgccag tgcggccaga ggctggtcag
    42001 ttcgctcatg actgtttccc ccgcagcagt gtcaactgat tgcgccagcg ttgttgcagg
    42061 ctcggccaga gtgtttggct gaccagcagc aggctgagca gcagggccag gcccaatggc
    42121 cagggataga gtggcaaggt tggccgcgcc agggtcggct tctgcgccac gggttccagg
    42181 cggtctaggg tcgcttcgat ggcttgcagc tcttcgctgt cgcgggcgcg gaagtattcg
    42241 ccgccggtgg cttcggcaat cgcacgtagg ctcggctcgt ccagatcgat gcccggatta
    42301 aagccgagca tgccgaggac gccgccttgt tccgggtcgg cgccgatgcc gatggggtaa
    42361 atggtgatgc cttcgtcggc ggccaggcgc gcggcggtca tggggtcaat ttccccgccg
    42421 ttgttggcgc cgtcagtcac cagcaccagt acgcgactct gcgccggacg ctcgcgcagg
    42481 cgtttgaccg ccaggccaat ggcgtcgcca atggcggtct gtggcccggc gatatttttc
    42541 tgcgcctcat ccagccaggt gtgcacggtc tgccggtcga aggtcagtgg cgactgcagg
    42601 taggcgcggc tgccgaacag gatcaggccg acccgatcac cctggcggcc gtcgataaag
    42661 tcgccgagca ggtgtttgac tagggtcagc cgactgactt cctggtcttc ccagagcatg
    42721 tcggagtaat ccatggagcc ggacacatcc accgccagca gcaggtcacg gccgctggcc
    42781 ggcaacggca gcggctcgcc cagccattgc ggacgcgcac tggcgagcag cagtaagagc
    42841 cagatcagcg caaacggtgc ctgttgccgc aaggctggca gtcgcacgcg ggcgcggcgt
    42901 ccgctcaagc cttccagctc cgccagaaag ctgattttca gcgccgcctc gccgctgtcc
    42961 gcaggcggta gccaggcgcg cagcagccag ggtagggggg cgatcaggaa gacccagggc
    43021 caggcaaact caaacatgtt tgcgaatcca gatcgacacc gcctggttga ggccggcgat
    43081 ggccttgtca tccaggttgc agtgcgggcg gtaggcgcct tccaccagaa tcatccagcg
    43141 agtcaggccg gcggcggggc aacggctgtc gagaaaagcc agccaggcgc ggctgctgag
    43201 aatatggctg tggctttgcg ggtaacgttc gcggcacagg cgtttgagga tggcattgag
    43261 ttgctgcagc caggggccgg catcgacgcc gtcgtagggt ttgctcaggc gttcgagttc
    43321 gttcaacgcg gcctggcgca acggatcaag cggcgcttcg gctgactgcg cgacagcctt
    43381 gcgtggcagg cgtttaagca gtttgaagcc agcccataac agcagtggca gaagtaccgc
    43441 gagtagccac cagccgggcg ccggcggcca ccagctgatt ggcgcagggg cgatcagcgg
    43501 ttcgagttga tccagcgggt tcacagggct ttcctcggcc gctgggcatt caggtgttcg
    43561 cgcagctgtt cgatcatctc gctctgggtg ttcagcgcca gcagcggcac gccgagtttc
    43621 tgcgccaggc gctgccagcg tgcctggcgg gcatcggcca ggctgcggta ggcctggcgc
    43681 agttcggcgt cctgggtatc cagctccagc tgcgcgccgt gttcggtaaa gcgcaacagg
    43741 ccggcattcg gcagggcatg gtcgagcggg tcggaaaggg gcagcaacaa caggtcggta
    43801 tgccgcgcca gcagcagcaa ctgctgctcg ctgctgtcgc tcaaggtgcg ctcatcacac
    43861 agaatcactg ccaggctgcc ggggcgcagc acctcgcggg cgcggcgtag ggccaggccg
    43921 aagctgtcgc ggccgccctg gctgtcattg ctcaaggcct gattggcctt ggccaggcgg
    43981 ctgagcagtt gcagcaggct ctgtttgctg cgccgtggtt tgatttcgtg gtgttccagg
    44041 tcgccaaata ccagaccgcc gatacggtcg ttgtggccca gagcggccca gccgatcagg
    44101 ctggcggcct gggcggcgag caccgatttg aacatcagcc cggagccgaa gaacagtcgc
    44161 ttgctctgct cgaccatgat gtagatcggc cgctcgcgct cttcatggaa cagcttggtg
    44221 tgcggctcct gggtgcgcgc agtaacgcgc cagtcgatgg tgcgcacgtc gtcgccgggc
    44281 tggtacacgc gcacctggtc gaagtccacg ccgcgcccgc gcagtttgga gtggtgcagg
    44341 ccaatcagcg ggctgcgccg cgctggggtg gaaaacagct gcacctcacg cacacggtgg
    44401 cgcatctcga tcagttcacc gaggctgacg cgaatgccgg gttgcgtggg ttggtcgtgc
    44461 atggtggtta ggcgaccgcc acgacgtcga gaatgcgctg caccacgcgg tcctggtcga
    44521 tgccggcggc ttccgcctcg aacgacagaa tgatgcggtg gcgcagcacg tcgaatagca
    44581 ccgcctggat atcttccggg ctgacgaaat cgcgcccggc caaccaggcg tgggcgcgcg
    44641 agcaacggtc cagggcaatc gagccgcgtg ggctggcacc gtaggcaatc cactcggcca
    44701 gttccgggtc gaatttggcc ggggtgcgtg aggccatcac cagttgcacc aaatactctt
    44761 ccaccgcatc ggccatatac aggccgagga tttcctggcg cgcggcaaag atcgcctgct
    44821 ggctgaccaa ctgctcgggc ttggtttcac cattgagcgc ttcgccacgg gcctgagcga
    44881 ggatcttgcg ctccacgctg gcgtcgggga agccgatctt cacgtgcatc aggaaacggt
    44941 cgagctgggc ttcgggcagc gggtaggtgc cttcctgctc gattgggttc tgcgtggcca
    45001 tcaccagaaa cagcggcgac aggtgatagg tactgcggcc gacgctgacc tggcgctcgg
    45061 ccatggcttc gagcagcgcc gactgcacct tggccggggc gcggttgatc tcatcggcca
    45121 gcaccaggtt gtggaagatc ggcccctgct ggaacacgaa gctgccggtt tccgggcgat
    45181 agatctcggt accggtgata tccgccggga gcaggtcagg ggtgaactga atgcggtgga
    45241 actcggcctc gataccttcg gccagctcct tgatcgcctt ggtcttggcc agaccgggag
    45301 cgccttcgac cagcatgtgg ccatcggcga gcaaggcaat cagcagcctt tcgatgagtt
    45361 tttcctggcc gaggatctga cttgaaagaa aatgtcgcag cgcgacaagc gcttcacggt
    45421 gttccatcgt ttggctcttt ctataggaag gtgcttgtcg tactcaacgc gacgacagtc
    45481 gagttctgcg aatagcgccc cgatgcgtga ggccgttaga tacagagccc gaactttaaa
    45541 gcatttgccg agttgcaggc tatgtgcgac tcgacatcta aggggggctg tggctgtttt
    45601 ggccgtgctg ggcgccgata tacgcgggcg cccgcaacgg gctgagggtc gtgcggggcg
    45661 ctgctgttca ctgcgcagtc agatctggaa ctgccgtgcg ctcaggctca atgtttcgac
    45721 ctgggttgag agccctcggg tgtgctggtc caaggtcatg cgcaggtttt tcgtttcgcc
    45781 ggtgtgcacg ttgatctcct cgattttgcc agcgatatcc tcagtggtgc tggagatctc
    45841 ttcgatagcg acgacgacct gattcatttc acccgagagt tgttccatcg agcaggtgat
    45901 ctgactgatt gaggccgcta cctgagcggc gtgttgctca ctctcggctg ccaaggatat
    45961 gccgctgctc atcagctcat gcatgttgtg cgtctgttct ttgaactcgc tgacaatcac
    46021 cgcgatgtta gtggttgccg acacggtttt ctgcgccagc gaacgtactt cctcagccac
    46081 gaccgagaag ccgcgcccgg cgtcaccggc gcgggcggcc tcgatggcgg cgtttagcgc
    46141 gagcaggttg gtttgatcgg ccaggctgtt gatcacatca ataatgccac tgaccttgtt
    46201 gctggactgg ccgagcaggg cgacctgggc atgggttgcc tggatcagct gcgataggcg
    46261 ccgcatgctt tcgacactgg cctcgatgat gatactgccc tggcgagcgg agtcgtaggc
    46321 tgtgtgggtg gcgttgccca catccgaggt gcgccgtgcc atctcgttga tggtggcgga
    46381 aatctgctgc gaggccgagg cggcttgttc ggtctgtatt tcaacctggc tgctgttatc
    46441 gccaaggctg cgcatggaat cgctaaggta gcggtgtagc tggctcagct cacggttggc
    46501 ttccacaacc tggccgacga tgtggccgat accagtgatc atgcggttgg aggcatcgcc
    46561 gagttggttg aattcatctt tgctgttgtt gccaacgttc agtgagccag tgaggtttcc
    46621 cgaggccacc tgcgacatga gtgcggtgac gctttgcagg cgggtgacga gcgtgcgcga
    46681 tgcctgattg agggtcaggg ttaggaacag ggcaaccaca acaaagctga gacccaatat
    46741 ccagttggcc gagttgcgtg cctgctgcgc tcgctggtcc gtgcgggcga gaatgccgtc
    46801 ttcgagcatg ctgccctggc tgtcgatctg ttgttcgatc tgctggccca attgtttaag
    46861 gcgctggttg ctgccgcgaa tctgttggat caggccgcct gcctcggtga atgcctggct
    46921 gaaacgggtg acggactgac cgatcgtgtt gtcttgccat tccagctcca cgaccttggc
    46981 ctgcagttcg tcgatagcgg ttttggcctg ttcggcgtac accggatcaa aggtcgatag
    47041 gtagtcgcgc tggccgctca gtgcgctggc aataaaggac cggatcaggc cgatggtgat
    47101 gggttccaag gccttcgccg cctcggccag cgttttccgt ttaccctcaa acgggcttag
    47161 gcccagttgc tggttgagtg ccagccaggc gcgctgttgg ctcagctcgt cgcgtaaaga
    47221 agcgacaatc gttgctgcgc tttggcttac ggcggggtcg agcaactgct ctgcgttggt
    47281 cagaagcgcc tgggcgtgct cttcgagtgc atccaggcgc tgctggtact gcgccagggt
    47341 gtcgggcgtg agttgttggc gcagggccgt aagcttcagc caatcattca ccagtgaggt
    47401 ggtggcactg gcgtaaccct tggcatcttc acgggcctgt agcgactcac tcaattgttt
    47461 attggcccac aacgaggcgg tggtcatcag gccaaggctg agcagggtga taacgatcag
    47521 cagacgaaac ttctgttgcc aggataggaa gaactgcacg gtaaagcctc atctcttttg
    47581 ttgttatggt acccggaatc ggcaggttgt cgtacaactt gaatattccg tttacttcac
    47641 aatcgcatgc agatgccggc aagtgcctgc tgctgccatt tatggaaaaa gcgaatgcgg
    47701 cagatgtctg acctctgcgg ccttgggctg gctctggccg tgctactgat caaggtgtta
    47761 actgcgaagc gacctcgacc agccggtacg tccggcagcg gagtaaccca tgcgcgctag
    47821 aggctgcggc ggcgtgcggg ggtgaaagaa aatgtcgcag cgccgtaagc gcctcagggt
    47881 gttcgatcgt taagctcaac cggtaaatgt gaccagcgag tcttgaacct cgccagagtt
    47941 aagtggcaga cgctttaatg caatcgctgc ctcctagact aacgttgaac aacaaggttg
    48001 cggcgaaggt cattactcgc gactgccttg atcgagtccc ctgtcgaata acaagccaaa
    48061 cggagcaaac ccatggcgtt ctttacggcg gccagcaaag ccgattttca gcatcacctg
    48121 caaacggcgc tggcgcagca cgtcagtgaa caggctctgc cacaagtagc cctgttcgcc
    48181 gagcaattct tcggcatcat cgccctcgag gagctgaccc agcgtcgcct ctctgatctg
    48241 gtcggctgca ccttgtcgtc ctggcggttg ctggagcgtt ttgaaccggc caagcccgaa
    48301 gtgcgggtat tcaaccccga ttatgaaaaa cacggctggc agtccaccca cactgccgta
    48361 gaaattctcc acagcgacat tccgttcctg gtcgactcgg tgcgcatgga gttgaaccgt
    48421 cgcggttaca gcatccacac cctgcagaac agcgtgttca gcgtgcgccg caacaagagc
    48481 ggcgagctgc aggaaatcct gcccaagggt gcccagggca aggatgtgca gcaagaagcg
    48541 ctgatgttcc tggagattga ccgctgcgcc aacgtcggcg agttgaagac tctggaaaaa
    48601 gacctgcacg aagtattcag cgatgtacgc ctgagcgtcg cggacttcca gccgatgaaa
    48661 gccaaggcca aggagctgct ggcctggctg gataaggcca agctgaaggt cgaaggcgcc
    48721 gagctggaag agatcaaggt attcatgagc tggttgctcg acgaccactt caccttcctc
    48781 ggttatgaag aattcaccgt cgccgacagc gccggcggcg gcagcatggt ctacgacgag
    48841 aagtccctgt tgggcatgtc caaacgcctg cgcagcggcc tcaaggctga tgaactgcat
    48901 atcgagcctg aagccgtggc ctacctgcgc gagccgcagc tgctgtcctt cgccaaggcc
    48961 gccgtaccga gccgcgtgca ccgtccggcg tacccggacc tggtatcgat ccgcgagctg
    49021 gacgccaagg gcaaggtgat caaggaatgc cgctttatgg gcctgtacac ctcggcggtg
    49081 tatgccgaga gcgtgtggaa cattccgttt atccgccgca aggtggcggt gatcaagcag
    49141 cgttccggtt tcgacagcag cgcgcaccta ggcaaagagc tggctcaggt attggaagtg
    49201 ctgccgcgtg acgacctgtt ccagacccct gtggatgagc tgttcaacac cactatggcg
    49261 atcgtgcaga ttcaggagcg caacaagatt cgcgtgttcc tgcgccgcga tccctatggc
    49321 cgtttctgct actgcctggc ttatgtgccg cgtgatgtgt actccaccga aacccgcatg
    49381 aagatccagc aggtgctgat ggagcgtctg caggctaccg attgcgagtt ctggaccttc
    49441 ttctctgagt cggtactggc gcgggtgcag ttcatcctca aggttgaccc gaaaaaccgc
    49501 gtgcagatcg acccgctgcg tctggaaaaa gaagttatcc aggcctgccg ttcgtggaag
    49561 gacgattaca ccagcctgat cgtcgaaagc ctcggcgaag cgcagggcac cgaagtgctc
    49621 agcgagttcc ccggcggctt cccggccggt taccgcgagc gtttcgcccc gcactcggcg
    49681 gtggtggata tgcagcacct gcttagcctg aacagcgagc gccaactggt catgagcttc
    49741 taccagccgc tgacccaggg cggtgagcag cagctgcact gcaagctgta ccacgccgat
    49801 acgccgttgc cgctgtcgga cgtgctgccg attctggaga accttggcct gcgcgtactt
    49861 ggcgagttcc cttacaaact gcgccgcgcc gatggccgcg agttctggat tcacgacttt
    49921 gccttcacct atgccgaagg cctggatgtc gacatccagc agctcaacga caccctgcag
    49981 ggcgctttcg tcaatatcgt tggcggtgct gcggagaacg acggcttcaa ccgtctggtg
    50041 ctgatggccg ccatgccgtg gcgtgatgtg gcgttgctgc gtgcctatgc gcgctacctc
    50101 aagcagattc gcctgggctt cgacctcagc tatatcgcca gcaccctggt taaccacgcc
    50161 gatatcgcca aggagctggt gcgcctgttc aagacgcgct tctacctggc gcgcaagctc
    50221 accgccgatg acctggaaga caagcagcag aagcttgaac aggcgattct cgccgcgctg
    50281 gataacgttg cggtgctcaa cgaagaccgc atcctgcgtc gctacctgga cctgatcaaa
    50341 gccaccctgc gtaccaactt ctatcagacc gatgcggccg ggcagaacaa gtcctacttc
    50401 agcttcaagc tcaacccgcg ggcaattcca gatattccgc ggccgacgcc gaagtttgaa
    50461 atcttcgtct actcgccacg tgtcgaaggc gtacacctgc gctttggcga cgtggcccgt
    50521 ggcggcctgc gctggtcgga ccgtgaggaa gacttccgca ccgaggtgct gggcctggtc
    50581 aaggcgcagc aggtgaagaa cgcggtcatc gtgccgatgg gcgccaaggg cggctttatt
    50641 ccacggcgta tgccgg
//
LOCUS       DASRUM010000210        42556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_624982, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000210 DASRUM010000000
VERSION     DASRUM010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 42556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42556
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624982"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..287)
                     /locus_tag="VFF22_13955"
     CDS             complement(51..287)
                     /locus_tag="VFF22_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17950.1"
                     /translation="MNTLTIIVLVAVVLAACALALWSWHNQRKLEKLDERINRQSELI
                     EQLDSVLDNPQLSEAEQLAKTEALLAKLQETRKP"
     gene            complement(284..778)
                     /locus_tag="VFF22_13960"
     CDS             complement(284..778)
                     /locus_tag="VFF22_13960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX17951.1"
                     /translation="MTTQPSAAFNLRAYQPHDLPAIVALFQASVSQLTTEHYDAAQRQ
                     AWSPEVADLPTWQTRLASLELLIAEDDQAIAGFIGFSLNGHIDLLFTAPGYARQGVAS
                     TLYAAAEQRLRATGVRELHTEASLVAQAFFTGQGFSVQQAQTVTRGAVILPRMLMRKT
                     LEPR"
     gene            complement(841..1731)
                     /gene="folE2"
                     /locus_tag="VFF22_13965"
     CDS             complement(841..1731)
                     /gene="folE2"
                     /locus_tag="VFF22_13965"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017903028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HZX17952.1"
                     /translation="MNALTLPDIAAQIPAHAQSLDWVGMQGIALPVNLAGQRINARAD
                     VGVSLDDGNARGIHMSRLYLALHALEQQELSPAALQQLLGEFLDSHQDLSQQASITLR
                     GEALLSRPALVSPLAGWKSYPFEVRARQDSWGFHVELQVEVAYSSTCPCSAALARQLI
                     QQRFVEDFADQPLDHAQLLAWLGSPQGIVATPHSQRSYATLDVRLQPQAAELPLLELI
                     DAAEQALGTAVQTAVKRADEQAFALANGQNLMFCEDAARRLHSALREQPAFSAFHLRV
                     VHAESLHAHDAVAASTWNWR"
     gene            1808..2236
                     /gene="dksA"
                     /locus_tag="VFF22_13970"
     CDS             1808..2236
                     /gene="dksA"
                     /locus_tag="VFF22_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HZX17953.1"
                     /translation="MTETPLTHVELLAQSADDYMNAAQQQFFRALLLSQRGELQTRIA
                     EEFEGLRERETSSDPADIGSAEEQRQWQLRLLEREKKLLDKIDEALDRLARGEYGWCR
                     ETGEPIGLKRLLLRPTASLCIEAKERQEQREKHLRHVDGD"
     gene            2241..3446
                     /gene="zigA"
                     /locus_tag="VFF22_13975"
     CDS             2241..3446
                     /gene="zigA"
                     /locus_tag="VFF22_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003231781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallochaperone GTPase ZigA"
                     /protein_id="HZX17954.1"
                     /translation="MTNRLPVTVLSGFLGAGKSTLLNAILKNRQGLKVAVIVNDMSEI
                     NIDGSEVQRDVSLNRAEEKLVEMSNGCICCTLREDLLEEVGRLARDGRFDYLLIESTG
                     ISEPLPVAETFTFRGEDGQSLADVARLDCMVTVVDGVNFLRDFHEAESLASRGETLGE
                     EDERSITDLLIEQVEFADVLLISKIDLISSHEREELMAILKGLNTHAEIVPMAMGAVP
                     LEKILNTGRFDFERAAQAPGWLKELRGEHVPETEEYGIASTAYRARKPLHPERFFNFI
                     NREWTNGRLLRSKGFFWLASKYQEAGSWSQAGGLMRHGFAGRWWRFVPKAQWPTDDEG
                     LQAIMKNWQTQTGDCRQELVFIGQNIDFTQLTTELDACLLTDAEMALGVDAWQALPDP
                     FGPWHEEAA"
     gene            3446..4090
                     /locus_tag="VFF22_13980"
     CDS             3446..4090
                     /locus_tag="VFF22_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1826 domain-containing protein"
                     /protein_id="HZX17955.1"
                     /translation="MLASVLRQPLQQVSSEQIDVLGEVLRDEVNLAVWQRQLPAQISD
                     FANALLAQGEPLGQSIVLELANPESTPNLSGLVDSYSDLPGQAAFLQDLSWLVGAFAC
                     LFDAKRIGLRLRILDKAMCPRFHVDHVPVRLITSYAGIGSEWLEEGVMARRRLGDPAA
                     EPSDAALIERAETGHVLLAKGEKWIGNEGGGLIHRSPQPPAGERRLLLTLDWLA"
     gene            complement(4226..4579)
                     /locus_tag="VFF22_13985"
     CDS             complement(4226..4579)
                     /locus_tag="VFF22_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HZX17956.1"
                     /translation="MRVFLLLSLFALSGLAWGEACVVHSQAERLDVKVCQQNRSIPPN
                     LFRTGFCQPQLKGQKVEVEFVEQCPMGAFGVCRNSTVGGTPYKQDVHYYGVASDANYL
                     KPFCEQQSKGVWMAR"
     gene            4711..5676
                     /locus_tag="VFF22_13990"
     CDS             4711..5676
                     /locus_tag="VFF22_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobW-like GTP-binding protein"
                     /protein_id="HZX17957.1"
                     /translation="MLKNIPTHVIAGPLGAGKTSLIRNLLAQHPADERWAVLINEFGL
                     IGLDAALLTTDDDGIALGEVAGGCLCCVNGVPFQVGLSRLLRKAKPHRLLIEPSGLGH
                     PLQLLEQLGEAPWAGVLAVQAPLLVLDAQALAAGVALPESQQATLGRAGLVLLNKAEG
                     LDDASKARIQAQLADRPIYWTRQGALPLAALPGFSAQADSDATPDNLPTAAAPLPQLW
                     RSPHEPICQIQQQAEGWSIGWRWHPSQQFELMRVQQWLSGLPWRRAKLVLQTNAGWLS
                     ANALNGQPLHWQNSEWRKDSRLELIFSEAQDVTHLQVGFAQCRLS"
     gene            5733..6611
                     /locus_tag="VFF22_13995"
     CDS             5733..6611
                     /locus_tag="VFF22_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX17958.1"
                     /translation="MQLLPWSHDCSAGFTLRGWHSPPSGKPVLHFLHGNGYCGRVYTP
                     MLQVLAEDFDLWLCDVQGHGESDHGGRFHGWNRSAELALEAFAAGRERFADVPVFALG
                     HSFGGVLTSLMLAQQPQLFSRAVLLDPVLFSPAMIGVMALSDVVGLSRRTTLASKALK
                     RRSNWPDRTAAYAALHGRGMFRGWTEEAFSAYIHHALKDSDKGVELKCRPSREADIFS
                     SMPKRLWPSLAKVTTPTQVLYGQRSYPFVAKSVARWCASNAQVSAHVVPGGHCFMQEQ
                     PADSAARVVHFLLQRD"
     gene            6617..6976
                     /locus_tag="VFF22_14000"
     CDS             6617..6976
                     /locus_tag="VFF22_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012020261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17959.1"
                     /translation="MSCREDVWLALSELWLDTELDQPALRYIAARLRASGLSLDELQH
                     IYGYEVAPQVWLNHWSVVGVWDGFEPQWLFAGCRRNQQGGHWHRLKCRLLRRPMTYGC
                     HAEWQQILAFLADPDFD"
     gene            complement(6983..7384)
                     /locus_tag="VFF22_14005"
     CDS             complement(6983..7384)
                     /locus_tag="VFF22_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HZX17960.1"
                     /translation="MIDLFDVFLLMLFATACAWLWHAHGLREHALGLVKQHCAKSDIE
                     LLDENVALRRLALLPDARGRKRLARVYGFEFTVTGEQRHIGSITLFGKQLGRIELAPH
                     PFREPPAPSAQVIEMQQWRSQRQDSDNNTQH"
     gene            complement(7478..7963)
                     /locus_tag="VFF22_14010"
     CDS             complement(7478..7963)
                     /locus_tag="VFF22_14010"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZX17961.1"
                     /translation="MEPGNAQLSMTVLMTPDMANFSGNVHGGTLLKYLDEVAYACASR
                     YAGCYVVTLSVDQVIFREPIHVGELVTFLASVNYSGRTSMEVGIKVITENIRERSVRH
                     TNSCFFTMVAVDERGKPAQIPALEPATADAKRRQRQALQRRQIRHELQQRYQALNEEK
                     N"
     gene            complement(8021..8779)
                     /locus_tag="VFF22_14015"
     CDS             complement(8021..8779)
                     /locus_tag="VFF22_14015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX17962.1"
                     /translation="MPLPVILISGAAGGLGRALAQRFHQGNWRVMVTDQNAAGLAQLC
                     HEHGLEGSITADLRTAEACRDVVSQVLARCGRLDALVNAAGIWREGPVEDFSEDDFDL
                     LMAVNLKAPFFLCAAAIPHLKDSRGAIVNISSDAGRQGNQNAAAYCASKGGLTLLGKT
                     LALDLAPYGVRCNTVSPGDIDTPMLKFQAERYGNGDPQGYYRSLLTKYPQGETARFVQ
                     PSEVAELVYFLCQPGAAAITGADMAIDMGLSAGI"
     gene            complement(8891..10258)
                     /locus_tag="VFF22_14020"
     CDS             complement(8891..10258)
                     /locus_tag="VFF22_14020"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HZX17963.1"
                     /translation="MQFANPQEARDFLAAHPEVRLIELMLIDANGIPRGKLLHRDELL
                     AIYENGRPLPSSILALTIQGEDVEESGLVWEVADADCWTYPLPSSLTLQPWRTTPTGQ
                     LQVSMHPTQGMPAAPADPRHVLVRAIDRLKADGYHPVMAVELEFYLLDKQRDANGRPQ
                     PALQMNGVRPQAPQVYGVYELEQLQPFLDDLYAACEVQGLPVRTAISEYAPGQVELTL
                     EHRFDALQAIDEGVRYKRLVKGVANKHGLQACFMAKPFSDQAGSGMHMHVSLADADGN
                     NLMASEDPHGTPLLKHAIGGMMATLNDVLAIFCPNANSYRRFQANSYAPLAKSWGVNN
                     RTVSFRVPGGPANSRHVEHRICGADANPYLAAAAILSGIHHGIQNQIDPGEEIIGNGY
                     EQARETLPTDWLTALRTLESSSWAKEALGEDFLKVFLAIKWAEFRQFMGEVGEQDWRW
                     YLTHA"
     gene            complement(10430..11821)
                     /locus_tag="VFF22_14025"
     CDS             complement(10430..11821)
                     /locus_tag="VFF22_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZX17964.1"
                     /translation="MTSSGISPSAVATFASRERERFIARNPKSVALAERARNSLYGGV
                     PMHWMADWSTPVPLFVERAKGARFYDVDGHEYIDFCLGDTGTMFGHSPDPIAKAIAEQ
                     GNNGLTTMLPGEDAVVCGELLAARFGLPFWQVTATATDSSRYVLRWARAITQRKTLLV
                     FDGCYHGTVDDVMVRCIDGETVPRSGLIGQAYDLTEYSRSIPFNDVAALEAALAQGDV
                     CALLCEPAMTNIGMVLPDPGFMQRCRELTRKYGALLIIDETHTISTDMGGCTKLWNLD
                     PDFFVVGKPIAGGVPCGIFGCSAAMAEAMTAARKRASEGSHGHGHSGMGTTLSANALA
                     MHCMRANLEQVMTPAAYAHMLPLAKRLADGFRSLINKHDLKWSVTELGARSEFQFCPI
                     SPKTGAEAEAAFHDELQMALHLYLINRGILITPFHNMTLCCPSTTAADVDKLIGMLDA
                     ALTELLAIPGARL"
     gene            11944..12714
                     /locus_tag="VFF22_14030"
     CDS             11944..12714
                     /locus_tag="VFF22_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZX17965.1"
                     /translation="MVFQQYVALISGAGSESGIGMAIARRLGRAGARVLLTASSARVE
                     QRVAELCAEGIQARGRAADLTDERQVGELLDWAQAQWGQIDILVNNAGMAMQGSPEPF
                     AELADMDLATWNLSLARNLGTAFLLTRAVLPGMRARGYGRIVNISSTTGTRGSNPGEA
                     AYSAAKAGMLGLSMGLALEVAKQGITVNSVAPGWIATGSSTEEERHAAEYTPMRRAGR
                     PEEVAAAVAFLASPEASYITGEVLVVDGGNCLIENKAP"
     gene            12835..13491
                     /locus_tag="VFF22_14035"
     CDS             12835..13491
                     /locus_tag="VFF22_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZX17966.1"
                     /translation="MADNLQDQLYQRIREGLLAGRFQPGERLKIRDLAAEWGTSPMPV
                     RAALQRLVAEGALEGEPQRSVRVPPMTRERYQNIFQVRLGLEGLAVELATPRLTPADL
                     AELRDCVARMDQAIEQRQVQAYLDANSQFHLHLYSACGNPVLLRSIESLWLQVGPFFN
                     QLFTGADLSLRLNDFHEDAFAAIEAGDAKAARQAMEQDLTYFARFLLNLLDLEAGPQR
                     "
     gene            complement(13534..13944)
                     /locus_tag="VFF22_14040"
     CDS             complement(13534..13944)
                     /locus_tag="VFF22_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HZX17967.1"
                     /translation="MEEVIEQLRELNEPVPVPLELPEEETLVEIQEQILIHLPFALRE
                     YLLQVSDVVYGRLEPVTASDPQSHTYLPEVAATAWSLGVPREFVPLCEDHGNYYVVEE
                     DGTVLLWEAETGEINEDETWESVWHWVRDVWLES"
     gene            14080..14673
                     /locus_tag="VFF22_14045"
     CDS             14080..14673
                     /locus_tag="VFF22_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17968.1"
                     /translation="MKSQTQEPEGGALSEEERLQLLTTAGKRNRLFILALAGVLGSLM
                     LVSVGLNIHSAFSAGDEPRIQVLEKQILRLEQQLAVQQTALSNQEELLASQQASQLTG
                     MFERVENPDSIAEVSKVLQAQERDYQQVMQGLKAGMRDLANMLPGSRSWLAHYEDAID
                     RAQLNSRQRSEDIQAWSDKAQQPAPAAKPPGTSVDPG"
     gene            complement(14677..15045)
                     /locus_tag="VFF22_14050"
     CDS             complement(14677..15045)
                     /locus_tag="VFF22_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZX17969.1"
                     /translation="MIGYTTVGTTDMEKAKSFYTPLLAEMGAKVVMDLGRIAFYGIAK
                     GQPMFAVCLPYDKQAPTPGNGQMIALPGGSREGVDTLYAKAIELGASCEGAPGERIPG
                     FYGAYFRDLDGNKLAFYQMG"
     gene            complement(15183..16034)
                     /locus_tag="VFF22_14055"
     CDS             complement(15183..16034)
                     /locus_tag="VFF22_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003253268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HZX17970.1"
                     /translation="MQRFLSIALAMFLSLGLTTMSFDAEAKRLGGGKSFGSAPSHQAR
                     QAPTQQTAAPATAGRQPAAASGASRWLGPLAGLAAGGLLASMFMGDGFEGLQIMDMLI
                     FGLIAFLLFRFLAARRRQAQPAAAGGVPYQREMPTQAAPSSIFGGSSAAAVIKPVINA
                     PAWFNEQSFINAGREHFLSLQQHWDANEMDKIGEFVTPQLLEFLSRERADLGDGFQST
                     YVDNLDVQLDGVDDLSDKTVATLTFTGVAKTSRFDQGEVFSESWRLERAVGDNQPWLV
                     AGIRQNG"
     gene            complement(16194..16772)
                     /locus_tag="VFF22_14060"
     CDS             complement(16194..16772)
                     /locus_tag="VFF22_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZX17971.1"
                     /translation="MKRATVSASQILDSALQLADVCGWERLHLFDVAADLGVGLDTIA
                     VHYREKDQLVEAWFDRADQALLARGTGADLLTLEPAKRLEELLIAWLDSLAAHRAVSR
                     QMLLYKLEPGHIHLQIGGLLRISRTVQWWREAARRETLHLCRIAEESLLTGVYLRCFI
                     HWLRHPEEDPADFRALLRRQLRCGPLLLLLRQ"
     gene            complement(16875..17981)
                     /locus_tag="VFF22_14065"
     CDS             complement(16875..17981)
                     /locus_tag="VFF22_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003297805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZX17972.1"
                     /translation="MIRRPLLLASALLAAIGLSGCKEEPAAPQATATPAAGTPAAESF
                     QWKMVTAWPKNYPGLGTAAERLAERVKTMSNGRLTIKVYAGGELVPPLEVFDAVSRGT
                     AELGHGAAYYWKGKVPAAQFFTAVPFGLSTSEMNAWLSKGGGQALWDEAYAPYGVKPM
                     VVGNTGMQMGGWYNKEINSLDDLKGLKIRMPGLGGEVLSKLGATTVNLPGGEVFTALQ
                     TNAIDATDWVSPYNDLAFGLHKAAKFYYYPGWQEPQAVLELLVNQKALDSLPADLQAI
                     LTEAARAAGQDMLDDYVYHNAMALDELKQQGTLLKRFPDEVLNAMQRESAVVLGELAA
                     QSELNGRIWASMQAFQAQVSQMHQLSEKELYNWR"
     gene            complement(18131..20320)
                     /gene="uvrD"
                     /locus_tag="VFF22_14070"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(18131..20320)
                     /gene="uvrD"
                     /locus_tag="VFF22_14070"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HZX17973.1"
                     /translation="MQNDLDQRLTSLNPAQHHAVTTPPGHQLVLAGAGSGKTRVLVHR
                     IAFLIQRLDVSPHSILSVTFTNKAAAEMRHRIEDVLGQSPAGMWVGTFHGLAHRLLRA
                     HWQEAGLAENFQILDSDDQQRIVKRVIRDLGLDEQRWPPKQAQWFINGQKDEGVRPAG
                     IQASGDLFLATMRGIYEAYEAACARAGVIDFSELLLRALDLWRDKPGLLAHYQRRFRH
                     ILVDEFQDTNAVQYAWLKHLAKGGESLMVVGDDDQSIYGWRGAKIENIQQFDSDFAGA
                     EIIRLEQNYRSTANILNAANALIANNQGRLGKELRTDVGDGEPINLYAAFNEHDEARY
                     VVETIEDALRKDGLKRSEIAILYRSNAQSRVLEEALLREKIPYRIYGGQRFFERAEIK
                     NAMAYLRLLDGRGNDAALERVINVPARGIGEKSVETIREYARAHDVSMWEAIRLMVAA
                     KTLPGRASSALAGFIELIENLAAKALSMSLHLMTQTVIEQSGLIAYHKAEKGEKAQAR
                     IENLEELVSAARTFENNEDDELSPLQAFLSHASLEAGDTQAAENEDSVQLMTLHSAKG
                     LEFPQVFLVGMEEGLFPHKMSLEEPGRLEEERRLAYVGITRAMNKLVITYAETRRLYG
                     SETYNKVSRFVREIPPQLIQEVRLSNSVSRPFGAVSRNMGGDNMFAGSAVPDTGFNLG
                     QRVQHSLFGEGTILNFEGSGAQARVQVNFESEGSKWLMLSYAKLEAL"
     gene            20543..23425
                     /locus_tag="VFF22_14075"
                     /pseudo
     CDS             20543..23425
                     /locus_tag="VFF22_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008078847.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            23500..24768
                     /locus_tag="VFF22_14080"
     CDS             23500..24768
                     /locus_tag="VFF22_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX17974.1"
                     /translation="MQPTSSAPAARPANATLILLASLYCAQGLPSGLLAHSLPVLLRQ
                     HGVDLALIGLLKLLALPWLLKVLWAPWVDRLASPRLGHHRGWILPLQLGVITVIASLA
                     LINPERLFDSHFLLLIGLLLLVNLAASTQDIATDGLTVRLLPERWRGLGNSLQVGGYK
                     VGMLVSGSGLLLAMDALGWHLSLGLVAVLLVLVTVPIWRFNERRELPFQPAQAEPAGP
                     GLLLRHYRGLLLQPGMLFWLAVLLSFKLGDALGSPMIKPMLVDQGWSNAELGQLTLIS
                     SLAGIAGAFLGGLLYARIGALRALLLFGLLQACGIAAMALLVNAGGSTGLVYLVALFE
                     QTADGMSTVALFAVMMRQCRPEHEGADFTLQASVQLLLAGLVGATSGWVAEQLGFASL
                     FISAGVLGLLALPIVLLYFWRGARLAPRGA"
     gene            24933..25607
                     /locus_tag="VFF22_14085"
     CDS             24933..25607
                     /locus_tag="VFF22_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17975.1"
                     /translation="MRKPASLSVGLRLLLASQAFAPLSVKLGLIQQLGAEKAALLAPH
                     MPPGQLRELIMAMPIEFAAAVATHLDPRLILDAYLSLPDSLHLEVARQLCADGAFATA
                     ALYAECLSAKQIKVLIYGINDVEHVLQIARYIVDMPLISESLRSFSTGYLCKLTEAAV
                     KDRNLPVAAQVLGGLSLVRQADVCAGLQPATLRQLLPMLLLISGDGLRKQLPEAVLQV
                     FEQQWV"
     gene            25630..25734
                     /locus_tag="VFF22_14090"
                     /pseudo
     CDS             25630..25734
                     /locus_tag="VFF22_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019727087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(25917..26963)
                     /locus_tag="VFF22_14095"
     CDS             complement(25917..26963)
                     /locus_tag="VFF22_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015275990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HZX17976.1"
                     /translation="MRSLTPEYLAALRFDGTQAATLRALGEYQGKQQLYVAQSPEALK
                     GLRQIAVVESTESSNRLEGVVVAPSRLKSLVIRNAAPKNRSEQEIAGYRDALALIHES
                     AAHMTFNEGVALQLHTMLYRYMPQAGGRWKATNNDIIERHPDGTSRLRFQPVAAHLTP
                     MAMADLTRYYSTALDQHLADPLVLVPLAMLDFLCIHPFPDGNGRMSRLLTLLLLYRFD
                     YAVGRYISLERIFEETKEGYYETLEASSQGWHQGQHNVKPWLDYFWGALLRAYREFEE
                     RVGTIERGRGSKGDRVRAEALGRTLPFSISEIEEACPGVSRDMVRLVLRAMKSEGLIE
                     STGKGRSAKWRRTT"
     gene            complement(27105..28592)
                     /gene="zwf"
                     /locus_tag="VFF22_14100"
     CDS             complement(27105..28592)
                     /gene="zwf"
                     /locus_tag="VFF22_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZX17977.1"
                     /translation="MAAAAALLNRVGVPTLSIPCDMLVFGGTGDLALHKLLPALYHLH
                     RAGRLHGDTRILALARSAHSRTAFQALAERHCRAQVARNDFDNDTWASFAARLDYFAM
                     DASQSADFGRLGKRLGEDHERVRIYYLATAPDLFEDIASHLKVASLAGPKARIVLEKP
                     IGHSLQSAQAINAAIGAVFDESRVFRIDHYLGKETVQNLMALRFANALFEPIWRAGHI
                     DHVQISVCETLGVENRGAYYEKAGAMRDMIQNHLLQLLCLVAMEAPVRFDAEAVRNEK
                     VKILEALKPITGLDVQDKTVRGQYTAGKIGGQEVPAYYFEKNVDSDSDTETFVAVQAE
                     IDNWRWAGVPFYLRTGKRLGHKTSEILIQFKPVPHQLFGGGEANRLLIRLQPEERISL
                     QLMGKSPGKGMHLQPVELDLNLADAFHKQRRWDAYERLLLDVIEGDSTLFMRRDEVEA
                     AWNWVDPIIKGWHQHYQSPRPYPAGSDGPEQASNLLEHHERHWME"
     gene            28704..29570
                     /gene="hexR"
                     /locus_tag="VFF22_14105"
     CDS             28704..29570
                     /gene="hexR"
                     /locus_tag="VFF22_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003213447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator HexR"
                     /protein_id="HZX17978.1"
                     /translation="MNLLQHIAQSRHLLRKSELKVADHVLLDPASVMHSSMAELAHSV
                     GISEPTIVRFCRAIGCTGFQDLKLKLAQSLAAGASFGQFAIHEDDSVADFSLKIFDTT
                     LHTLIEVREKLDTQALQQAIAAIANAQRVEFYGFGASGAVAADAQHKFFRLLLTAAAY
                     SDPHMQAMSAVTLKPTDVAICISQSGRSKDLLITANLVREAGATLITLCPSQTPLADL
                     ATVNLAIDVQEDTEIYTPLTSRIAHLVVIDVLAMGVAMARGPGLVNHLKSVKRSLRSL
                     RLSPKAHKSSDD"
     gene            29817..30041
                     /locus_tag="VFF22_14110"
     CDS             29817..30041
                     /locus_tag="VFF22_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008366994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZX17979.1"
                     /translation="MSAYLDKAQNHANSEARARRKVIDQRRMEYRRAIESYAEQRQLQ
                     QQLADYPELIAVNYLAGKQALARQNVQPGR"
     gene            complement(29983..30945)
                     /locus_tag="VFF22_14115"
     CDS             complement(29983..30945)
                     /locus_tag="VFF22_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014822038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX17980.1"
                     /translation="MRKSLLRMTFRQIQIFRSVCSHLSYSRAAEEMSLSQPAVSLQIR
                     QLEELIGQPLFDYVGKKLYLTEAAEALQRASADIFGRLESLDMQLSDLQGSLQGQLGL
                     AIESSAKYFVPHLFAAFRQQYPEVNLQLVVTNHSQALRRLNANRDDLLIMSRVPTDMN
                     LEFLPFLTNPIIAVAPPEHPLCSQPALKLQDLTAFPLLVREAGSGTRQACEEYCHQKR
                     AHFAQTLEVGSMEGQCEGVIAGLGLALLPRHAVRRELECGALRELPVSELPLLRSWCL
                     VHPRGKYLSPVAQAFFAFVRDQRSLINALAERFAAPTPACPPGS"
     gene            31134..32552
                     /locus_tag="VFF22_14120"
     CDS             31134..32552
                     /locus_tag="VFF22_14120"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HZX17981.1"
                     /translation="MIKKILIANRGEIAVRIVRACAEMGIRSVAVYSDADRHALHVKR
                     ADEAHSIGEEPLAGYLNPRKLVNLAVETGCDALHPGYGFLSENAELADICVERGIKFI
                     GPSAEVIRRMGDKTEARRSMIAAGVPCTPGTEGNVADIAEALREGDRIGYPVMLKATN
                     GGGGRGIRRCNSRDELEQAYPRVISEATKAFGRAEVFLEKCIVNPKHIEAQVLADSFG
                     NTVHLFERDCSIQRRNQKLIEIAPSPQLTPEQRAYIGDLSVRAAKAVGYENAGTVEFL
                     LAEDGELYFMEMNTRVQVEHTITEEITGIDIVREQIRIASGLELSVKQEDIIHRGFAL
                     QFRINAEDPKNNFLPSFGKITRYYAPGGPGVRTDTAIYTGYTIPPYYDSMCLKLVVWA
                     LTWEEAMDRGLRALDDMRVQGVKTTAAYYQEILRNPEFRSGQFNTSFVEAHPELTEYS
                     IKRNPSHLAIAIATAIAAHAGL"
     gene            32569..34383
                     /gene="oadA"
                     /locus_tag="VFF22_14125"
     CDS             32569..34383
                     /gene="oadA"
                     /locus_tag="VFF22_14125"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007971704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HZX17982.1"
                     /translation="MSKTKKITVTDTILRDAHQSIIATRMRLDDMLPICGKLDKVGYW
                     SLEVWGGATFDSCVRFLKEDPWERLRKLKAGLPNTRLQMLLRGQNLLGYRHYSDDVVR
                     AFVAKAAVNGIDVFRIFDAMNDVRNLRVSIEAVKAAGKHAQGTIAYTTSPVHTVEAFV
                     KQAKAMQAMGIDSIAIKDMAGLLTPYATADLVRALKAEVDLPVFIHSHDTAGMGSMCQ
                     LKAIEAGADHIDTAISSFAWGTSHPGTESMVAALKGSEYDTGLDLALIQEIGLYFHAV
                     RKKYHQFESEFTAVDTRVQVNQVPGGMMSNLANQLKEQGALNRINEVFAEIPRVREDL
                     GFPPLVTPTSQIVGTQAVFNVLAGERYKTITNEVKLYLQGRYGLAPGKINEQLRKQAI
                     GSEEVIEGRPADLLKPEMAKLREEIGALAKSEEDVLTYAMFPDIGRKFLEERAAGTLT
                     PEVLLPIPEAGGMAPAGGEGVPTEFVVDVHGESYRVDITGVGVKNDGKRHFYLSIDGM
                     PEEVVFEPLNDFVAGSSGKRKQASAPGDVSTSMPGNIVDVLVKVGDTVKAGQAVLISE
                     AMKMETEVQAPIAGTVKAVHVAKGDRVNPGDVLVEIEG"
     gene            34690..35631
                     /locus_tag="VFF22_14130"
     CDS             34690..35631
                     /locus_tag="VFF22_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HZX17983.1"
                     /translation="MSLDPVVLFFVFGLLAGLLKSELKLPPALYETLSIILLLAIGLH
                     GGVELAEQASWQLLGQAAWVLGLGVALPLLAFAVLRGLGFDRVNAAAVAAHYGSVSAG
                     TFAVVVAYMLAKGIEFESYMPLFVAILEIPAILVGILLAKGIARDTDWGELGREIFLG
                     KSIMLLLGGLIIGAIAGKEAIKPLEPFYTSMFKPVLALFLLEMGLIASGQLGSLRQFG
                     LRLLGFALLMPLLGALIGALLGRFMGLSLGGTAVLATLAASASYIAVPAALRLALPEA
                     NPSLSLTASLGITFPFNIIVGIPLYLMLAEQLLAWGL"
     gene            35633..35935
                     /locus_tag="VFF22_14135"
     CDS             35633..35935
                     /locus_tag="VFF22_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3240 family protein"
                     /protein_id="HZX17984.1"
                     /translation="MDAHIRTLLTVICEAALEKKLVADLEQLGAPGWTISDARGRGGR
                     GVRSAGWDTEGNIRVEIICTRDVAERIAAHLQARYYANFAMVCYLAQVEVLRAEKF"
     gene            36177..36701
                     /locus_tag="VFF22_14140"
     CDS             36177..36701
                     /locus_tag="VFF22_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZX17985.1"
                     /translation="MVNYRRARVAGGTYFFTLTLKNRRSDLLVRHIDLLRDALRQVKR
                     RRPFTLLAVVVMPDHVHLLLQLPDMDTNYSARLRDFKMLFVKALRAAGGPIRSDVWGE
                     AGIWQARFWEHTIRDERDLAAHIDYIHFNPVKHGHVSRAVDWPYSSFHRYVRQGLLSA
                     DWAGGAGLELAGDD"
     gene            36871..37779
                     /gene="glsB"
                     /locus_tag="VFF22_14145"
     CDS             36871..37779
                     /gene="glsB"
                     /locus_tag="VFF22_14145"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HZX17986.1"
                     /translation="MQALLNEILDQVRPLIGQGKVADYIPALADVPANQLGIAVYSND
                     GELYCAGDADTAFSVQSISKVFSLVQAIQHSGEDIWQRLGHEPSGQPFNSLVQLEFER
                     GRPRNPFINAGALVICDINQSRFAAPVLSMRDFVRRLAGNPQIVVDRRVADSEYQHRA
                     RNAAAAYLMQSFGNFHNDVEAVLRSYFSYCALRMSCADLARAFCFLANEGFCKHSGAQ
                     IISARQSKQVNAIMATSGLYDEAGNFAYRVGLPGKSGVGGGIIAVVPGQFSVCVWSPE
                     LNAAGNSLAGIKALELLSERIGWSVF"
     gene            complement(37991..39346)
                     /locus_tag="VFF22_14150"
     CDS             complement(37991..39346)
                     /locus_tag="VFF22_14150"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005770054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HZX17987.1"
                     /translation="MAATRIWIKNPLAIFTANNLDAAGGLVIENGVISELLKAGQQPS
                     QPCEQSFDAREHVVLPGLINTHHHFYQTLTRAWAPVVNQPLFPWLQTLYPVWARLTPE
                     KLALASKVALAELLLSGCSTAADHHYLFPGGLENAIDVQVEAVRELGMRAMLTRGSMS
                     LGQADGGLPPQQTVQQGEVILADSQRLIQDYHQRGDGAQIQIALAPCSPFSVTQEIMR
                     QSAELAEELDVRLHTHLAETLDEEDFCLQRFGLRTVDYLDSVGWLSSRTWLAHGIHFN
                     PDEIARLGQAGTGICHCPSSNMRLASGICPTLDLQAAGAPIGLGVDGSASNDASNMIL
                     EARQALYIQRLRYGAEKITPELVLGWASKGSAKLLGRSDIGELAVGKQADLAFFKLDE
                     LRFSGSHDPISALLLCGADKADRMMIGGQWRVIDGQIEGLDVAKLIADHRQAAKELIA
                     G"
     gene            complement(39516..39971)
                     /locus_tag="VFF22_14155"
     CDS             complement(39516..39971)
                     /locus_tag="VFF22_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX17988.1"
                     /translation="MPIRIPVEIRPISDADHAAWLPLWQGYQRFYMTEIAAATSDITW
                     QRFLDPAEPMHAALAWHEGQAIGLVHWIYHRSTWTPGDYCYLQDLFIAKDVRSSGAGR
                     KLIEHVYQEAQAQGCSRTYWLTHESNSRAMLLYERIAERSGFIQYRKVF"
     gene            complement(40029..41015)
                     /locus_tag="VFF22_14160"
     CDS             complement(40029..41015)
                     /locus_tag="VFF22_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HZX17989.1"
                     /translation="MGCKKLLVLYTGGTIGMQMSAEGLAPASGFEARLRAQQSREAQP
                     LPEWIFRELLPPIDSANMNQSNWLAMVAAIRAGIEQDGCDGVLLLHGTDTLAYSAAAL
                     SFLLLGLPVPVILSGSMLPAGAEGSDAWSNLFGAMRALQAGVTPGVHLYFNGELLHGA
                     RVSKLRSDAFDAFHVLPRQRQSPRAESIPASIDYRQPRQPVNLAVLPFYPGIQASHVR
                     ALLDSGVQGLLLECYGSGTGPADDAELLAVLKAAHERGVVLAAISQCPHGHVEFGVYA
                     AGSQLASAGLISAGGMTREAALGKLFALLGAGLSQAEVEHWYALDVCGERAD"
     gene            complement(41095..42537)
                     /locus_tag="VFF22_14165"
     CDS             complement(41095..42537)
                     /locus_tag="VFF22_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HZX17990.1"
                     /translation="MLDILNDLIWSKLLIVMLIGLGLYFTIASRFVQFRYFGSMFRIF
                     AEAFQRQPGQLSSFQALMLSVAGRVGAGNIAGVSVAIMLGGPGAIFWMWVVALVGMAT
                     SYFECSLAQLYKRREDDGTYRGGPAFYIQHGLGQRWLGIVVSILLLVTFGFGFNAVQS
                     FTVASSMHDTFGLPTYASGAILTVVMGLIIFGGIKRIAKFADVLVPVMAFSYIAMALF
                     VIGSNVDAIPATLALIFKSAFGLEPAFAGGIGAAILMGVKRGLFSNEAGLGSAPNVAA
                     VAEVKHPVAQGIVQSLSVFIDTIILCSCTALIILLSGVYQPGTEMAGVVLTQTAVASV
                     VGEWGRVFVTVALLLFVFTTLIYNYYLGENALGFFSKKRGPVVVYRVLVVALVLWGSV
                     QDLGTVFAFADVTMGLLAICNLIALVLLFKVGLRLMRDYDSQIKAGVESPVFDAKNFA
                     DLDLDPAAWPNAAHSAPATQAEIGNAVHQR"
BASE COUNT         7683 a        12662 c        13766 g         8445 t
ORIGIN      
        1 gcatccccgc ggttcctaca gggttgtgac agttgaaccc tgtcacaacc ctagggctta
       61 cgggtttcct gcagcttggc cagcaaggct tcggttttcg ccaactgctc ggcttcactg
      121 agctgcgggt tgtccagcac gctgtcgagc tgttcgatca gctcgctctg ccggttgatc
      181 cgttcatcca gcttctccag cttgcgctgg ttgtgccagc tccacagggc cagggcgcag
      241 gcggccagca caacggctac cagcacgata atcgtcaggg tgttcatcgc ggctccagag
      301 ttttgcgcat cagcatgcgc ggcaaaataa cagcgccgcg cgtcaccgtc tgtgcctgct
      361 gcacagaaaa tccctggccg gtaaaaaacg cctgagccac caggctggct tcggtatgca
      421 gctcgcgcac acccgtcgcc cgcagacgct gctctgcggc ggcatacaag gtgctggcga
      481 caccctggcg ggcatagccc ggtgcggtga acagcagatc aatatgccca ttcaggctaa
      541 agccgataaa accggcgata gcttgatcat cttcggcgat aagcaactca agtgacgcca
      601 aacgtgtttg ccaggtgggc aagtccgcaa cctcgggaga ccaggcctga cgctgcgctg
      661 cgtcgtagtg ctcggtggtc aactggctga ccgatgcctg aaacagcgcg acgatggcgg
      721 gcaggtcgtg aggttggtag gcccgcaggt tgaatgcggc ggacggctgg gttgtcatcg
      781 gagtggtcta caagggtttg ccctgcaggt tagcgccgcg tatgggccag gtaaatcgcc
      841 tcagcgccag ttccacgtgc tggcggcaac ggcgtcgtgg gcgtgcaggc tttccgcgtg
      901 caccacgcgc aggtgaaagg cgctgaaggc cggttgttcg cgcagggcac tgtgcaggcg
      961 gcgggcggcg tcttcgcaga acatcaggtt ttgcccgttg gccagggcga aggcctgttc
     1021 gtcggcacgc tttaccgcag tctgcacggc ggtgccgagg gcctgttcgg cggcatcgat
     1081 cagttccagc agcggtagct cggcggcttg tggttgcagg cgtacgtcca gggtggcgta
     1141 gctgcgctgg ctgtgcggcg tggcgacaat gccctggggc gagccgagcc aggccagcag
     1201 ttgcgcgtga tccagcggct ggtctgcgaa gtcctcgaca aagcgttgct ggatcagctg
     1261 gcgcgccagg gcggcggagc aggggcaggt cgaggaatag gccacttcca cctgcagttc
     1321 cacgtgaaac ccccatgaat cctggcgtgc acgcacttcg aagggatagg acttccaccc
     1381 ggcaaggggg ctgaccagcg ccggacggct gagcaaggcc tcgccacgca aggtgatgga
     1441 cgcctgctgc gacaggtcct ggtggctgtc gagaaactcg cccagcagct gctgcagcgc
     1501 cgccgggctt agctcttgtt gttcgagagc atgcagcgcc aggtacaggc gcgacatatg
     1561 aatgccgcgg gcattgccgt catccaggct gaccccgacg tcggcgcggg cattgatgcg
     1621 ctggccagcc aggttgactg gcagcgcgat gccttgcatg ccgacccagt ctagggactg
     1681 tgcatgggcg ggtatttgcg cggctatgtc cggcaaggtc agtgcgttca tcagaaatct
     1741 gccgctcggt tgtgggttta aatgttatgt tataacaagt aaattacaaa accagcgaat
     1801 cctgatgatg accgaaacgc cactcaccca cgtcgagctg ctcgctcagt cggcggatga
     1861 ctatatgaac gccgcacagc agcaattctt ccgcgctctg ctactgagcc agcgcggcga
     1921 attgcaaacg cgcattgccg aagagtttga aggcctgcgc gaacgcgaaa ccagcagcga
     1981 cccggccgat atcggcagcg ccgaagagca acgccagtgg cagctgcgcc tgttggagcg
     2041 ggaaaagaag ctgctcgaca agatcgacga agccctcgac cgcctggctc gcggcgaata
     2101 cggctggtgc cgggaaaccg gtgagccaat cggcctcaag cgtctgctgc tgcggcccac
     2161 cgcaagcctg tgcatcgaag ccaaagaacg tcaggaacaa cgcgaaaaac acctgcgcca
     2221 cgttgatgga gactgaaacc atgaccaatc gccttcccgt aaccgtgctg tccggctttc
     2281 tcggtgccgg taaaagcacc ctgctcaacg ccatcctgaa aaaccgccag ggcctgaaag
     2341 tggcggtaat cgttaacgat atgagcgaga tcaacatcga tggcagcgaa gttcagcgtg
     2401 atgtcagcct gaaccgcgcc gaagaaaagc tcgtggagat gagcaacggc tgcatctgct
     2461 gcaccttgcg cgaggatctg ctggaagaag ttggccgtct ggcccgcgat ggccgcttcg
     2521 actatctgct gatcgagtcc accggcattt ccgaaccgct gcctgtggct gagaccttca
     2581 ccttccgcgg cgaagacggc caaagcctgg ccgatgtggc gcgcctggac tgcatggtga
     2641 cggtggtcga cggggtgaat tttctgcgcg atttccacga ggccgagagt ctggccagcc
     2701 gtggtgaaac cctgggcgaa gaagatgagc gctcgattac tgacctgctg atcgagcagg
     2761 tggaattcgc cgatgtgctg ctgatcagca agatcgacct gatcagcagc cacgagcgcg
     2821 aagagttgat ggccattctc aagggcctca atacacacgc cgaaattgtg ccgatggcca
     2881 tgggcgcggt gccgctggaa aagatcctca ataccggccg cttcgacttt gaacgcgccg
     2941 cccaggcacc gggttggctc aaagagttgc gcggtgagca tgtgccggaa accgaggaat
     3001 acggcattgc ttccacggct taccgggcgc gcaagccgct gcaccccgag cgcttcttca
     3061 atttcatcaa tcgcgaatgg accaatggcc gtttgctgcg cagcaagggc ttcttctggc
     3121 tggccagcaa gtaccaggag gccggcagct ggtcccaggc gggcggtttg atgcgccatg
     3181 gctttgctgg gcgctggtgg cgctttgtgc ccaaggcgca atggccgacc gacgacgaag
     3241 ggctgcaggc gattatgaaa aactggcaga cgcagaccgg cgactgtcgc caggagctgg
     3301 tgtttatcgg ccagaacatc gacttcaccc agctgaccac agagctggat gcctgcctgc
     3361 tgaccgatgc ggaaatggcc ctcggtgtcg acgcctggca agcgctgccc gacccgttcg
     3421 gcccctggca tgaggaggct gcctgatgct tgcatctgtt ctgaggcagc cattgcagca
     3481 ggtcagcagc gagcagatcg acgtgcttgg cgaggtgctg cgtgatgagg tcaacctggc
     3541 tgtctggcag cgccagttgc cggcgcagat cagcgatttc gccaacgcct tgctggccca
     3601 gggcgagccg ctggggcaat ccatcgtgct cgagctggcc aacccggaga gcacgccgaa
     3661 cctcagtggc ctggtcgaca gttatagcga tctaccaggc caggcggcat ttttgcagga
     3721 cctgagttgg ctggtcggtg cctttgcctg cctgtttgac gccaaacgca tcggcttgcg
     3781 cctgcgcatt cttgacaagg ccatgtgccc gcgctttcac gtcgaccatg taccggtgcg
     3841 gctgatcacc agctatgcgg ggattggcag cgagtggctg gaagaaggtg tgatggcgcg
     3901 tcgtcgtctt ggtgatccgg ctgccgagcc cagcgatgct gcgctgatcg aacgtgccga
     3961 gaccggccat gtgctgctgg ccaagggcga gaagtggatc ggtaatgagg gcggtggctt
     4021 gatccaccgc tcgccgcaac cgccggccgg tgagcggcgc ttgttgctga cactggactg
     4081 gctggcctga atttcaggca aaaaaaatag gctgcggaga aagcagcctt aagcaccaga
     4141 aaagcacacc ggcgcgtgac ccggcatgca ggggtgaggt acccaaggcc actagaacat
     4201 aaacagggtt acgctttgat gacggctagc gcgccatcca cacgcccttg ctctgctgct
     4261 cgcagaaggg cttgaggtag ttggcgtcgc tggccacgcc gtaatagtgc acatcctgct
     4321 tatagggcgt gccaccgacc gtggagttgc gacatacgcc gaaagcgccc atcgggcact
     4381 gctcgacaaa ctccacttcg accttctgcc ccttcagctg cggttggcag aagccggtgc
     4441 ggaacaggtt gggcgggatg ctgcggttct gctggcagac cttcacatcc aggcgctcgg
     4501 cctggctatg caccacacag gcttcacccc aggccaggcc ggacagtgcg aacaggctca
     4561 gcagcagaaa aacgcgcatc aattgaccct ctgaaaatat gccggcgact ctagcatgct
     4621 gtttttaatc agtctgcgta gggccgcatc ccgccgttag aatgaccgca gtagaacctt
     4681 atttttgatg gacagtggca ggcgccgatc gtgctgaaga atatccccac ccatgtaatt
     4741 gccgggccgc ttggtgccgg caagaccagc ctgatccgta atctgctggc gcagcacccg
     4801 gctgatgaac gctgggcggt gttgatcaat gagttcggcc tgatcggcct ggacgctgcg
     4861 ctgctgacca ctgatgacga cggcattgcc ctcggcgaag tggccggcgg ttgcctgtgc
     4921 tgcgttaacg gcgtgccgtt ccaggttggc ctcagccgct tgttgcgcaa agccaaaccg
     4981 catcgcctgc tgatcgagcc atccggcctc ggccatcccc tgcaactgct ggaacagctc
     5041 ggcgaagcgc cttgggctgg cgtcctggcc gtgcaggccc cgttgctggt gctggacgct
     5101 caagccctgg ccgcaggtgt tgcgctgccg gagagccagc aggcgacgct tggccgcgcc
     5161 gggctggtgc tgttgaacaa ggccgaaggg ctggatgatg ccagcaaggc gcgcattcag
     5221 gcgcagctgg cggatcggcc gatttactgg acccgtcagg gtgcattgcc actggcagcg
     5281 ttgcctggct ttagcgccca ggccgatagc gacgctacgc cggataacct gcccacggcc
     5341 gctgcgccct tgccgcaact gtggcgcagc ccgcatgagc cgatctgcca gattcagcaa
     5401 caggctgaag gttggagcat cggctggcgc tggcatccca gccagcagtt cgagctgatg
     5461 cgcgtacagc agtggctgag cggcctgccc tggcgacggg ccaagctggt gttgcaaacc
     5521 aacgccggct ggctgtcagc caatgccctg aacggccagc ctttgcactg gcaaaacagc
     5581 gagtggcgca aggactcgcg cttggagctg atctttagcg aggcgcagga tgttacccac
     5641 ctgcaggttg gttttgcgca gtgccgtttg tcctgaagct cagggccttc tggtattgat
     5701 aaactgcggt cacttttttc tggatagcgc ccatgcaact gcttccctgg tcccacgact
     5761 gctcggccgg ttttacccta cgcggctggc attcgccgcc ttcgggtaaa cccgtgctgc
     5821 attttctgca tggcaacggt tactgcgggc gggtttacac gcccatgctg caagtcttgg
     5881 ccgaggattt cgatctgtgg ctgtgcgatg tgcagggcca tggcgagagt gatcatggcg
     5941 ggcgtttcca tggttggaat cgcagtgccg agctggcgct ggaggcgttt gctgctggcc
     6001 gtgagcgctt tgccgatgta ccggtgtttg ccttgggcca cagctttggc ggggtattga
     6061 ccagcctgat gttggctcag caaccgcagc tgtttagccg tgcggtattg ctcgacccgg
     6121 tgctgttcag cccggcgatg attggagtga tggcgctgtc cgatgtggtc ggtctgagtc
     6181 ggcgaaccac cctcgctagc aaggcgctca agcggcgcag caactggccg gatcgcaccg
     6241 ccgcgtatgc agccctgcat ggccgtggga tgtttcgcgg ctggaccgaa gaggcctttt
     6301 ccgcctatat ccatcacgcg ctgaaagaca gcgacaaggg tgtcgagctg aaatgccggc
     6361 caagccgtga ggcggatatt ttcagctcca tgcccaagcg cctgtggccg tcgctggcca
     6421 aggtaacgac gccgacccag gtgctttacg gccagcgcag ttaccccttt gtcgccaagt
     6481 cggtggcgcg ctggtgcgcc agcaatgccc aggtcagcgc tcatgtggtg ccgggcgggc
     6541 actgctttat gcaggagcaa ccggctgaca gcgccgcgcg cgtggtgcac tttctgttgc
     6601 agcgcgacta agccggatga gctgccgtga ggatgtctgg ctggcgctct ccgaactgtg
     6661 gctggacact gaattggacc agccggcgct ccggtatatc gccgcgcggc tgcgcgccag
     6721 tgggttgagt ctggatgagt tgcagcatat ctacggctat gaggtggcgc cgcaggtctg
     6781 gcttaaccac tggtcggttg tcggggtgtg ggatggtttc gagccgcaat ggctgtttgc
     6841 cggttgccgg cgcaatcagc agggcgggca ctggcaccgg ttgaaatgcc gtctgctgcg
     6901 ccggccgatg acctatggct gtcacgccga atggcagcag atcctggcgt ttctggctga
     6961 tccggacttc gactagtcgg ccttaatgct gcgtgttgtt atccgagtcc tgacgctgac
     7021 tgcgccactg ctgcatttca atcacctgag cgctgggcgc cggcggttca cggaaaggat
     7081 gcggggccag ctcgatgcgt cccagctgtt tgccaaacag ggtgatgctg ccgatatgcc
     7141 gttgctcgcc ggtgacggtg aactcgaagc catacacccg tgccagtcgc ttgcgcccgc
     7201 gcgcatcggg caacagggcg agccgacgca gggcgacatt ttcatcaagc aactcgatgt
     7261 cggatttggc gcagtgttgt ttcaccaaac ctagggcgtg ttcacgcaga ccatgggcat
     7321 gccatagcca ggcgcatgcg gtggcgaaca gcatcagcag aaatacatcg aagaggtcga
     7381 tcatggcggc tccggcggca gagtgcagag cttaacagac gccgcctctg cagcctgagc
     7441 cgggattaac cgtgcaaacg cagagggttt atttcgctca gttcttttct tcgttcaggg
     7501 cctgatagcg ctgttgcaat tcgtggcgga tttgccggcg ctgcagggcc tggcgttggc
     7561 ggcgtttggc gtctgcagtg gcaggctcaa gggcggggat ctgcgcgggt ttgccccgtt
     7621 catccaccgc gaccatggtg aagaagcagc tgttggtgtg gcgtaccgag cgttcgcgga
     7681 tattttccgt gatcactttg atgccgactt ccatcgaggt gcgcccggag tagttgaccg
     7741 aggcgagaaa ggtcaccagt tcgccaacgt ggatcggctc gcggaagatc acctggtcca
     7801 ctgacagagt caccacgtaa caaccggcat agcggctggc acaggcgtag gcgacttcat
     7861 caaggtattt gagcagggtg ccgccgtgca cattgccgga gaaattggcc atgtccgggg
     7921 tcatcagtac ggtcatcgac agctgggcgt ttccgggttc catgcaggct ccaggcttgg
     7981 ggggtaggca gtggacgttt gccacagcct accagacacc tcagatcccc gccgacaagc
     8041 ccatgtcgat ggccatgtcg gcaccggtga tggccgccgc acctggctgg cagaggaagt
     8101 acactagttc ggccacttcg ctgggttgga cgaagcgcgc cgtctcaccc tgtggatatt
     8161 tggtcagcag gctgcggtag tagccctgcg gatcgccgtt accgtagcgc tcggcctgga
     8221 atttcagcat cggcgtgtcg atatcgcccg gcgacacggt gttgcaacgc acgccgtagg
     8281 gtgccaggtc gagcgccaga gtcttgccca gcagggtcaa gccaccctta ctggcgcagt
     8341 aggccgcggc attctggttg ccctggcgtc cggcatcgct ggagatattg acgatggcgc
     8401 cgcggctgtc cttgaggtgg ggaatcgccg cggcgcacag gaagaatggt gccttgaggt
     8461 tgaccgccat aagcagatcg aagtcgtcct cgctgaagtc ctccaccggc ccttcacgcc
     8521 atataccggc ggcattcacc agggcatcca gacgcccgca acgggccagc acctggctga
     8581 cgacatcacg gcaggcctcg gccgtgcgca gatcggcggt gatcgaacct tcaagcccat
     8641 gctcatggca gagttgagcc aggccggcgg cgttctggtc ggtgaccatc acacgccagt
     8701 taccctggtg gaaacgctgg gccagggccc gaccgaggcc gccagcggca ccgctaatca
     8761 atatgacagg tagaggcatg atgggctcca taggttgagt tgagcgtaga taagctcagc
     8821 cggctggcgg gcttgtcgct tagccggcct ggctcgcatc ggactgcgcc gctgtgcggc
     8881 tcaacccgac ctacgcgtgg gtcaggtacc agcgccagtc ctgctcgccg acttcgccca
     8941 tgaattgacg gaactcggcc cacttgatcg cgaggaatac cttgaggaag tcctcgccca
     9001 gcgcttcctt ggcccagctg gagctttcca gggtacgcag ggcggtcagc cagtcggtgg
     9061 gcagggtttc acgggcctgt tcgtagccgt tgccgatgat ttcctcgccc ggatcgatct
     9121 ggttctggat gccgtgatgg atgccggaaa gaatcgccgc cgctgccaga taggggttgg
     9181 cgtcggcacc gcagatgcgg tgctcgacat gccggctatt ggccgggccg cctggtacgc
     9241 ggaacgacac agtgcggttg ttcacccccc agctcttggc cagcggcgcg tagctgttgg
     9301 cctggaagcg gcgataagag ttggcgttgg ggcagaaaat cgccaggaca tcgttgagcg
     9361 tggccatcat gccgccaatg gcgtgcttga gcagcggcgt gccatgtggg tcttcgctgg
     9421 ccatcaggtt gttgccgtcg gcgtccgcca aactcacatg catatgcatc ccgctgccgg
     9481 cctggtcgct aaacggcttg gccataaagc aggcctgtag tccgtgcttg ttggccacgc
     9541 ccttgaccag gcgcttgtag cgcacgcctt cgtcgatggc ctgcagcgcg tcgaagcggt
     9601 gctccagggt cagttcgacc tggccgggtg cgtactcgga gatcgccgtg cgcaccggca
     9661 ggccctgtac ttcgcaggcg gcgtagaggt catcgaggaa cggctgcagt tgttccagct
     9721 cgtacacgcc atacacctgt ggcgcctgcg ggcgcacgcc gttcatttgc agcgccggtt
     9781 gcgggcggcc attggcgtcg cgctgtttgt ccagcaggta gaactctagc tccaccgcca
     9841 ttaccgggtg gtagccgtcg gccttgaggc ggtcgatggc gcgcaccagc acatgccgtg
     9901 gatcagccgg ggcggccggc atgccttgcg tcgggtgcat gctcacctgc agctggccgg
     9961 tgggcgtggt gcgccagggc tgcagggtga ggctgctggg cagtgggtag gtccagcagt
    10021 cggcatcggc gacttcccag accaggccgc tttcttcgac gtcctcgcct tggatggtca
    10081 gcgccaggat tgagctgggc agcggccggc cgttctcgta gatggccagc agctcgtcgc
    10141 ggtgcagcag cttaccgcgc gggatgccgt tggcatcgat cagcatcagt tcgatcaggc
    10201 gcacttccgg gtgggcggcg aggaagtcac gggcttcttg gggattggca aattgcatgg
    10261 tgtgatctcg cgtagggtgt gccgtgcgca caaacggtgc acacggccca tgattgttct
    10321 gatggttcca acgctctgcg tgggaaccac tccggggacg ctctgcgtcc cttgcgacgc
    10381 agagcgtcgc tgcctgcgct cccacgccgg agcgtgggag cgatcttatt cagagccggg
    10441 cgccggggat ggccagcagc tcggtcaggg ccgcatcaag catgccgatc aacttatcca
    10501 catcagccgc tgtggtgctc gggcagcaca gggtcatatt gtggaacggg gtgatcagga
    10561 tgccgcggtt gatcaggtac aggtgcaggg ccatctgcag ctcgtcgtgg aacgccgctt
    10621 cggcctcggc gccggtcttc ggcgagatcg ggcagaactg gaattcgctg cgcgcgccca
    10681 gctcggtcac cgaccacttt aggtcgtgct tgttgatcag gctgcggaag ccgtcggcca
    10741 ggcgcttggc cagcggcaac atatgcgcgt aggcggccgg cgtcatcacc tgctccaggt
    10801 tggcgcgcat gcagtgcatg gccagggcgt tggctgacag ggtggtgccc atgccgctgt
    10861 ggccatgccc atggctacct tcgctggcgc gtttacgcgc tgcagtcatg gcctcggcca
    10921 tcgccgcgct gcaaccgaag atgccgcacg gcacgccgcc ggcaatcggc ttgccgacca
    10981 cgaagaagtc cggatcgagg ttccacaact tggtgcagcc acccatatcg gtggaaatgg
    11041 tgtgggtctc gtcgatgatc agcagcgcgc cgtatttacg cgtcagctcg cggcaccgct
    11101 gcataaatcc cggatcgggc aggaccatgc cgatattggt catcgccggc tcgcagagca
    11161 gggcgcagac gtcgccttgg gccagtgcag cttccagcgc ggcgacatcg ttgaacggga
    11221 tcgagcggct gtactcggtc aggtcatagg cctggccgat caggccggag cgcggcacgg
    11281 tctcgccatc gatacagcgc accatcacat catccacggt gccgtggtag cagccgtcga
    11341 acaccagcag ggtcttgcgc tgggtaatcg cccgagccca gcgcagcaca tagcggctcg
    11401 aatccgtggc agtggcggtg acctgccaga acggcaggcc gaagcgcgcg gccagcagct
    11461 caccgcagac caccgcgtcc tcgcccggca gcatggtggt caagccgttg ttgccttgct
    11521 cggcgatggc cttggcgatc gggtccggcg agtggccgaa catggtgccg gtgtcgccca
    11581 ggcagaaatc gatgtactca tgaccgtcga cgtcatagaa gcgcgcgccc ttggctcgtt
    11641 cgacgaacag cggcacaggg gtcgaccagt cggccatcca gtgcatcggt acgccgccgt
    11701 acagcgagtt gcgcgcgcgc tcggccagag ctacggattt cgggttgcgc gcaataaagc
    11761 gttcgcgctc gcggctggcg aaggttgcta cggcggatgg gctgatacca ctggaagtca
    11821 tgttgaacac ctcgttcgaa tttctgcaca tgctattttg tgatcacaga aaacacaagt
    11881 attggtttga tcaggcttta ttgttagatt acagccatat ttgtgatcac aggattttgc
    11941 gaaatggttt tccaacagta tgtagcgctg atcagcggcg caggcagtga atcgggtatc
    12001 ggcatggcca tcgcccgccg tctgggccgt gcaggcgcgc gggtgctgtt gaccgccagc
    12061 agtgcacggg tcgagcaacg ggtcgccgag ttgtgtgctg aaggtattca agcgcgtggc
    12121 cgcgccgctg acctgaccga tgaacggcag gtcggcgaat tactcgactg ggcgcaagcg
    12181 cagtgggggc agatcgatat tctggtaaac aacgccggca tggccatgca gggcagcccg
    12241 gagccgtttg ccgagttggc ggacatggac ctggcaacct ggaacctgtc ccttgcgcgc
    12301 aacttgggca cagccttttt actaacccgt gcggtgctgc cgggcatgcg tgcgcgcggc
    12361 tatgggcgca tcgtcaatat cagctcgacc accggcaccc gtggcagcaa ccccggcgag
    12421 gcggcctaca gtgcagccaa ggccggcatg ctgggcctgt ccatggggct ggcgctggaa
    12481 gtggccaagc agggtattac ggttaatagc gtggcgccgg gctggattgc tacgggttcg
    12541 agtaccgaag aagagcgcca tgcggcggag tacacgccga tgcgccgcgc cggacgacct
    12601 gaggaagtgg cggcagcggt ggcctttttg gcgtcgccgg aggccagcta tatcaccggc
    12661 gaagtgctgg tagtggatgg cggtaactgc ctgatcgaga acaaggctcc gtgagccatt
    12721 agaattcatt gcgccgctct agtgcggcct ttttcaatat aaggaatgtc catgcgcaac
    12781 tgctcgctga ccctcgccca gctccccgca ccaccgctac ggggctgaac gaccatggcc
    12841 gataacctgc aggaccagct ttaccaacgt attcgtgaag gtctgctggc tgggcgtttc
    12901 cagcctggcg agcgtttgaa gattcgtgac ctagccgccg agtggggcac cagcccgatg
    12961 ccggtacgcg ccgccttgca gcgcctggtc gccgaaggcg cgctggaagg cgagccgcag
    13021 cgttcggtgc gcgtaccgcc gatgactcgc gagcgttacc agaatatctt tcaggtgcgc
    13081 ttgggccttg aaggcctggc cgtggagttg gccacgccac gcttgacccc tgccgatttg
    13141 gccgagttac gcgactgtgt ggcgcgcatg gatcaggcca tcgagcagcg gcaggtgcag
    13201 gcatacctgg atgccaacag ccagttccat ctgcatctct acagcgcctg cggcaacccg
    13261 gtgctgctgc gctcaatcga atcgctgtgg ctgcaggtcg ggccgttttt caaccagctg
    13321 ttcacgggcg ccgacctgtc gctgcgcctt aacgacttcc atgaggatgc ttttgccgcc
    13381 atcgaagccg gtgacgccaa ggcggcgcgc caggccatgg aacaggacct gacttacttt
    13441 gcgcgcttcc tgctcaacct gctcgacctg gaggcgggcc cgcagcgtta aaaccgcgct
    13501 ggagagtcgt tacgctgacg cacagagcag gtctcagctt tccagccaga cgtcgcgcac
    13561 ccaatgccag accgattccc aggtttcgtc ttcgttgatt tcaccggttt cggcttccca
    13621 cagcagcacg gtgccgtctt cttccaccac gtagtagttg ccgtggtctt cacacagcgg
    13681 cacgaactcg cgcggcacgc cgagggacca ggcagtggcg gcgacttccg gcaggtaggt
    13741 gtgcgattga ggatcgctgg cggttaccgg ctccagacgg ccgtagacca cgtcgctgac
    13801 ctgcagcagg tactcacgca gggcgaaggg caggtggata agaatctgct cctgaatctc
    13861 caccagggtt tcttcttccg gcaactccag cgggacgggc accggctcgt tgagttcgcg
    13921 caactgttcg atgacttctt ccacggtgtc tgcctcttta attacgggta atgcgggagc
    13981 gtcttatacc ctagacgggt ctaagcaaaa agctggcaaa gtgctactgt ttagctcagt
    14041 ggagtgtcaa ttttttaacg tcataaaaat ggtcttgtta tgaaaagcca aacgcaagag
    14101 cccgaaggcg gtgcactcag cgaggaagag cggttacagc tgctgaccac ggctggcaag
    14161 cgcaaccgtc tatttatcct ggcgttggcc ggggtgctgg gtagcctgat gcttgtttcg
    14221 gtcgggttga atattcacag cgcgttcagc gccggtgatg aaccgcgtat ccaggtactg
    14281 gaaaagcaga tcctgcggct tgagcagcaa ctggcggtac agcagaccgc gttgagcaat
    14341 caggaagaac tgctggccag ccagcaggca tcgcagctga ccggaatgtt cgagcgcgtg
    14401 gaaaatccgg acagcatcgc cgaagtgagc aaggtgctgc aggcccagga gcgcgattat
    14461 cagcaggtga tgcagggcct gaaggccggt atgcgtgacc tggccaatat gctgccgggc
    14521 tcgcgcagct ggctggcgca ttacgaggac gcgattgacc gcgcccagct caacagccgg
    14581 cagcgcagcg aggatattca ggcatggagc gacaaggccc agcagcctgc cccggcggcc
    14641 aagccgccgg gtaccagcgt cgatccgggt tagttcctag cccatttggt agaaggccag
    14701 cttattccca tccagatcac ggaaataggc gccgtagaag cccggtatgc gctcgccggg
    14761 tgcgccttcg cagcttgccc ccagttcgat ggctttggcg tagagcgtgt ccaccccttc
    14821 gcgtgagccg ccgggcaggg cgatcatctg gccattacct ggggtaggcg cttgcttgtc
    14881 atagggcagg cagacggcaa acatcggctg acccttggcg atgccgtaga aggcgatgcg
    14941 acccaagtcc atcacgacct tggcgcccat ttcagccagc aagggggtgt agaacgactt
    15001 ggctttttcc atgtcagtgg tgccaaccgt ggtgtatccg atcattgctc agtcctcgtg
    15061 gtgggtaggt tcgtgcggtt cagattgcac cttgagtgga tcgcgcgcca gcctatgcat
    15121 ttgctgaatg atggacaaag aaaacccggg gcttgcccgg gttttctttg ttgcagcagg
    15181 tattaaccgt tctggcggat accggcaacc agccatggct ggttgtcacc gacggcacgc
    15241 tccaggcgcc agctttcgct gaacacttcg ccttggtcga agcgcgaggt cttggccacg
    15301 ccggtgaagg tcagggtggc gacggtcttg tcgctgaggt catcgacgcc atcgagctgc
    15361 acatcaaggt tgtcgacgta ggtggactgg aagccatcgc ccaggtcggc acgctcgcgg
    15421 ctgaggaatt ccagcagctg cggggtgacg aactcgccga tcttgtccat ctcgttggca
    15481 tcccagtgct gttgcagact gaggaagtgc tcacgaccgg cgttgataaa gctctgttcg
    15541 ttgaaccagg ccggggcgtt gatcaccggc ttgataacgg ctgcggcgct gctgccaccg
    15601 aaaatcgagc taggtgcggc ctgggtcggc atttcgcgct gatatggcac accaccggcg
    15661 gcagccggct gggcctggcg acgacgggca gcgaggaagc ggaacagcag gaaggcgatc
    15721 agaccgaaga tcagcatgtc catgatctgc aagccttcga agccgtcacc cataaacatc
    15781 gaggccagca gaccaccggc ggccagaccg gccaatgggc ccaaccagcg cgaggcgccg
    15841 ctagccgcag caggctgacg accggcggtc gccggtgcag cggtttgctg ggttggtgcc
    15901 tggcgagcct ggtggcttgg cgcggaaccg aaggatttgc cgccgcccag tcgtttggct
    15961 tcggcgtcga aactcatggt ggtcagtccc aggctgagga acatggccag ggcgatgcta
    16021 aggaaacgct gcatggttag ctgtctcttg gtgattggat ctacgcgcgg catcttgccc
    16081 ggcaagatcg ccgctggcta ggcctagaat gtttccggct tttgcccgag tcgctgcgta
    16141 gggcgtgccg cctggcgagt gtcttggtgc gcgcggcgca ccctacggga tcactattgg
    16201 cgcagtagca acagcagcgg cccgcagcgt aactgccggc gtagcagggc gcggaagtcg
    16261 gcggggtctt cctcggggtg gcgcagccag tggataaagc agcgcaggta gacgccggtc
    16321 agcaggcttt cttcagcgat acggcacagg tgcagggttt cccgccgcgc cgcttcacgc
    16381 caccactgta cggtgcgact gatgcgcagc aggccgccaa tctgcaggtg aatatgcccc
    16441 ggttccagtt tgtacagcag catctgcctg ctgactgcgc ggtgcgccgc caggctgtcg
    16501 agccaggcga tcagcaattc ttccaggcgt ttggccggct ccagcgttag taggtctgcg
    16561 ccagtgccgc gggccagcag ggcctggtcg gcgcggtcga accaggcttc caccagctga
    16621 tccttctcgc ggtaatgcac ggcgatggta tccagaccca cgcccaggtc ggcggccaca
    16681 tcgaacagat gcaggcgctc ccagccgcag acatcagcca gttgcagggc gctatcaagt
    16741 atttgtgatg cggagacagt tgcgcgtttc atcggctcac ctcgccaagc agtatgggcg
    16801 gcgagtgcag tcgggcctgc ctgaaacgac aaacggaacc cgaaggttcc gcttgtgctg
    16861 attgcagcga ggtgtcagcg ccagttgtac aactctttct ccgacaactg gtgcatctgg
    16921 ctgacctggg cctggaaggc ttgcatcgag gcccagatac ggccgttcag ttcgctctgc
    16981 gcggccagtt caccgaggac tacggcggac tcgcgctgca tggcattgag cacttcgtca
    17041 gggaagcgct tgagcaaggt gccttgctgc ttcagctcat ccagggccat ggcgttgtgg
    17101 tagacgtagt catccagcat gtcctggccg gcggcgcggg cggcttcggt gaggatcgcc
    17161 tgcagatcgg ctggcaggct gtcgagggcc ttctggttga ccagcagctc cagcaccgcc
    17221 tgcggttcct gccagcctgg gtagtagtag aacttggccg ctttgtgcag gccgaaggcc
    17281 aggtcgttgt aggggctgac ccagtcggtg gcgtcgatgg cgttggtctg cagggcggta
    17341 aacacttcgc cgccgggcag gttgacggtg gtggcgccga gcttgctcag cacttcgccg
    17401 cccagtcctg gcatgcggat cttcagaccc ttgagatcat ccagcgagtt gatttccttg
    17461 ttgtaccagc cgcccatctg catgccggta ttgccgacca ccatgggttt tacgccatag
    17521 ggggcatagg cttcgtccca caatgcctga ccgccgcctt tgcttagcca ggcgttcatc
    17581 tcgctggtgg acaggccgaa gggcacggcg gtgaagaatt gcgctgccgg caccttgccc
    17641 ttccagtaat aggccgcgcc atggcccagt tcggcggtgc cgcgggatac ggcgtcgaac
    17701 acttccagcg gcggcaccag ctcgccaccg gcatacacct tgatggtcag gcggccgttg
    17761 ctcatggtct tgacccgctc ggccaggcgt tcggcggcgg tgcccaggcc gggatagttc
    17821 tttggccagg cggtgaccat cttccactgg aagctttctg cggcaggcgt ccctgctgcg
    17881 ggcgtggcgg ttgcctgcgg cgcggcaggc tcttccttgc aaccgctcag gccgatggcg
    17941 gcgagcaggg cgctggcgag cagcaaaggg cggcggatca ttggggctcc ttattgtttg
    18001 aatggacgat caatcacgct gggcaccttg tagagcggcc catggccgcg acagcaggca
    18061 cccgaaaaag catcgcgggc atggcccgct cctacaggtt gtttgcagct cgtagcccgg
    18121 acagaaaacg ttaaagcgct tccagcttgg catagctgag catcagccac ttgctgcctt
    18181 cgctctcgaa attcacctgc acccgcgcct gggcgccgga gccttcgaag ttgaggatgg
    18241 tgccctcgcc gaatagcgaa tgctgcacgc gctggccgag gttgaagccg gtgtccggta
    18301 cggcgctgcc ggcgaacata ttatcgccac ccatattgcg gctgaccgcg ccgaacggcc
    18361 ggctgacgct attgctcagg cgcacttcct gaatcagctg cggcggaatc tcgcggacga
    18421 agcgcgacac cttgttgtag gtctcgctgc cgtacaggcg gcgcgtttcg gcgtaggtga
    18481 tcaccagctt gttcatggcg cgggtgatac cgacgtaggc cagacggcgc tcttcttcca
    18541 gacggccggg ctcttccagg ctcatcttgt gcgggaacag gccttcttcc atgcccacca
    18601 agaacacctg ggggaactcc aggcccttgg cgctgtgcag ggtcatcagt tgcacgctgt
    18661 cttcgttttc cgccgcctgg gtgtcgccgg cttccagcga ggcgtggctg aggaaggctt
    18721 gcagcgggct cagttcgtcg tcttcgttat tctcgaaggt gcgcgctgcg ctgaccagtt
    18781 cttccaagtt ttcgatgcgc gcctgggctt tttcgccttt ctcggccttg tgataggcga
    18841 tcagtccgct ttgctcgatg acggtctggg tcatcaggtg cagcgacatg ctcagcgcct
    18901 tggcggcgag gttttcgatc agttcgataa acccggccag cgcgctcgaa gcccggccag
    18961 gcagggtctt ggcggcgacc atcaggcgaa tcgcttccca catcgacaca tcatgggcac
    19021 gggcgtattc gcggatggtc tcgacgctct tctcgccgat gccgcgcgcc ggcacgttga
    19081 tcacccgctc cagcgcggcg tcgttgccgc ggccgtcgag caggcgcagg taggccatgg
    19141 cgttcttgat ttcggcgcgt tcgaagaagc gctggccgcc atagatgcga tagggaatct
    19201 tctcgcgcag cagggcttct tcaagcaccc gcgactgggc gttggagcgg tagaggatgg
    19261 cgatttcgct gcgcttgagg ccgtccttgc gcagggcgtc ctcgatcgtc tcgaccacgt
    19321 aacgcgcttc gtcgtgctcg ttgaacgcgg cgtacagatt gatcggctcg ccgtcgccga
    19381 catcggtgcg cagttcttta cccaggcggc cctggttatt ggcgattagc gcgttggcgg
    19441 cattgaggat gttcgccgtg gagcggtaat tctgctccag gcggatgatc tcggccccgg
    19501 cgaaatcgct gtcgaactgc tggatgttct cgatcttcgc cccgcgccag ccgtagatcg
    19561 actggtcgtc gtcgccgacc accatcaggc tttcgccgcc tttggccaga tgcttgagcc
    19621 aggcgtactg cacggcgttg gtgtcctgga actcgtcgac caggatatgc cggaagcgcc
    19681 gctggtaatg cgcaagcaac cccggtttgt cacgccacaa gtcgagggcg cgcagcagca
    19741 gctcggagaa gtcgatcacc ccggcacgcg cgcaggcggc ctcataggct tcgtagatgc
    19801 cacgcatggt cgccaggaac agatcaccgc tggcctggat gccggccggg cgcacgcctt
    19861 cgtctttctg cccgttgata aaccactgcg cctgcttagg cggccagcgt tgttcatcca
    19921 ggccgaggtc gcggatcacc cgtttgacga tgcgctgctg gtcgtcggaa tcgagaatct
    19981 ggaagttttc tgccaggccg gcttcctgcc aatgggcgcg cagcaggcga tgggccaggc
    20041 catggaaggt gccgacccac atgccggccg ggctctgccc gagcacgtct tcgatgcggt
    20101 ggcgcatctc ggcggcggcc ttgttggtaa aggtcaccga caggatgctg tgtggcgaga
    20161 cgtccagccg ctgaatcaga aaggcaatac ggtgcaccaa cacgcgggtc ttgccggagc
    20221 cggcaccggc cagcaccaac tgatggccag gcggcgtggt aaccgcatgg tgctgggctg
    20281 ggttgaggga ggtgagtcgc tgatcaaggt cattttgcat cgcggcattc tagggggctg
    20341 cgtcaggcag ggcaaaccag gctgttgtgc gggatttatt ggacgaccgg tgagtcagct
    20401 cggcaggcgc atacaggcgt ggccgtgctc atcgaaagtt aggtgtttgt agtgtgaatc
    20461 agcgcacatc tggcttgggc gaagcgtcta gctcgcgtat gctgcgccga cgtttaggca
    20521 accacatata agaaaatcgc ctatgactgt atcgactcca tacgctgacg cttctggctt
    20581 aaagccgact tccggacggg aagtggcgca gctatttgca accgaaatag ccgccgaacg
    20641 cacccgcatg ctctaccagg gctcgcaggt gccgaccctg tttatgctgc ttagcggtgt
    20701 ggtgtgtgcc ttcctgttat ggggtgcgct gccaccggtg ctgctggtca gttggtcggt
    20761 ctggctggta attctcgccg tgctgcgtct ggtgcaggtg cgcgccttca acaatgcctt
    20821 gcccagccgt caggctgaac gccattggca ccgcctgttc ctgttcggcg cgagcgtttc
    20881 cgggctgacc ctggcgtttg ccgctatcgc cctggtgccg gcggatgtgt tctacctgca
    20941 ggcagtggtt tatggcctga ttgctgcggc gattctctct gccagcgtgg cttatgccat
    21001 cagctttact gccttcctca gttttgcctt gccttgcctg ctgccttcgg tgggctatct
    21061 gctgctcagt gataacccgc tacagcaggg ttggggactg ctcggcagta ttctgatgtt
    21121 ggccttgctg gttgtggcct ggcaggttaa tcgcctggtg cagcgcagcc tgttgcagcg
    21181 ttttcacaac caggcgctga tcagcaacct ggagcacgcc aagttgcagg ccgaagggct
    21241 caacggcgag ttggcccatg aagtggaaca gcgccgccgc gccgagcgcg acttgcgtca
    21301 ggcccatggt gagctggaaa tgcgcgtggc tgagcgcact ctggaactgg atgaaaccac
    21361 ctatgccctg agcaagagcc gcgcgcgcct gaccctggcc atcgaagcca gcgagctggg
    21421 cttgtgggac tgggatctgc agagtgatga ggtgcaccac tcgcgcctca aggaaatctt
    21481 cggtctggag ccccatgaag tacaggtgat gctgcgcgac ctcaagccgc gcctgcatcc
    21541 ggaggatctg ccggtgctgc gtcgagcgct ggtcgagcat atgaaggggc gcagcgacgg
    21601 ctatcaggtc gaataccgta tcaagcatgc ccagggccac tgggtatgga tcgaggaccg
    21661 tggccgcgcg gttgagcgcg gtgccgatgg ccgcgtgcag cgcatgctcg gcacccgccg
    21721 tgacatcagt gcgcgcaagg tgcaggaaca gcagcaacag ttggccgcca cggtgttcga
    21781 ggccgccagc gaaggcatcg tgattctcga ccctgattac cgcctgctgt cggtcaacca
    21841 ggccttcagc gacgtcaccg ggtatcgcca ggacgaggtg gtcggcaaga gcgcgttcag
    21901 cctgatcagc agtggcgaca gccgccgaca gtaccagatg atccgccagg aattggagaa
    21961 cagcggcacc tggcgcggcg agctgatcga aacgcgcaag aacggcgagc tgtatccgca
    22021 gtggctgcag ctcaacgtgg tgcgtgatgc gcggggcagt gtcagtcata tcgtcggctt
    22081 cttcgccgac ctatcgtcgc ggcgcgaggc cgaagagcgc ctgcgctacc tgtcgcacta
    22141 cgacgagctg accggcctgg ccaaccgcag ccttttcaag gagcgtctac acgaggccag
    22201 ccagcgcgca cgtcagggcg gacgcagcat cgccctgctg catgtcgacc tggaccgctt
    22261 caagctgctc aacgacagcc tcggccatga agtggccgac cagctgctgc gccaggtcag
    22321 ccgccgtctg actcaggcag tgccggaagc cgacaccctg gcgcgcttgt ccggtgacga
    22381 attcgccatc ctgctcgact cctacggcag cctctccagc ctggcgcgcc tcgccagccg
    22441 cttgctgagc aaactgcgcg tgccgatgac cgtgggtggc catgaattag tggtcagcgc
    22501 ctccctgggc atcagcttgt tgccggataa cgcgcgggag atttccgccc tggtcagcca
    22561 ggccaatatg gccatgcaac atgccaagca cctgggcggc aataccttcc agttcttcac
    22621 cgacaacctg caggcctgca ccctgtagcg cctgcaactg gaaaaccagc tgcgcaaagg
    22681 catcaacgaa ggccagctgg aagtgttcta ccagcccaag ctgtgcctgg cctccgacag
    22741 cctgaatgcc gccgaggcgc tggtgcgctg gcgccacccg gaactgggcc tggtgccgcc
    22801 gagcgacttt atcggcctgg ccgaagaaac cggtcttatc ggtgccattg gcgaattcgt
    22861 cttgcgtcag acgtgcctgc aggcgcgggc gtggcaggct caagggctgg cgcagatccg
    22921 cgtgtcggtg aacctctcgg tgcatcagct gcgccagggc aacctgctca gcctggtgcg
    22981 ccaggtgctc gatgaaaccg gcctggaggc gcagtacctt gagctggagc tgaccgaaag
    23041 ccagctgctg gataatgtcg agagcgtaat cagcaccttc cggcaactgc gcgaactggg
    23101 cgtcaaactg gccatcgacg acttcggcac cggctattcc tcgctcagct acctcaagcg
    23161 cttcccggtg gactacgtga agatcgacca aacctttatc cgcgacctca cccccggcag
    23221 cgaagacgcc gccatcaccc gtgcgatcat cgccatggcc cacagtctgg aactcaaggt
    23281 ggtcgccgag ggtgtggaaa cccaggcgca aatggacttc ctcaagagcc agcgttgtga
    23341 cgagcttcag ggctacctga tcagcaaacc ggtgcccgcc gaagagttcg cccagttgct
    23401 gcgcgagcag gcggacaagt tctgattggc cggccggcgg cttgtctgcc gccggactgg
    23461 ccgctactgt cgcgctttcg ctgactcgca ggatgctcca tgcagcccac ttcttccgcc
    23521 cccgctgctc gcccggccaa tgccaccctg atcctgcttg cctcgctgta ttgcgcccag
    23581 ggcctacctt cgggtttgtt ggcgcattcg ttaccggtgt tgctgcgtca gcacggcgtt
    23641 gatctggcgc tgatcggcct gctcaagctg ctggcgttgc cctggttgct caaggtgctt
    23701 tgggcaccgt gggtggaccg gctagcgtcg ccgcggttgg gccatcaccg cggctggatt
    23761 ctgccgttgc agcttggcgt tattacggtg attgcgagcc tggcactgat caatcccgag
    23821 cggctgtttg acagccactt cctgttgctg attggtctgt tgctgctggt caatctggct
    23881 gcttcgactc aggacattgc cactgacggc ctgaccgtac ggctgctgcc ggaacgctgg
    23941 cgcgggctgg gtaacagttt gcaggtgggt ggttacaagg tcggcatgct ggtcagcggc
    24001 agtggtctgc tgctggcgat ggatgcgctg ggttggcacc tgagtctggg gctggtggcg
    24061 gtgctgctgg tgctggtgac agtgccgatc tggcgcttta acgaacggcg cgagctgcca
    24121 tttcagccgg cgcaggcgga gccggccggg ccgggattgc tgctgcggca ttaccgtggc
    24181 ctgctgctgc agcccggtat gttgttctgg ttggcagtgt tgctgagttt caaactgggt
    24241 gatgccctgg gctcgccgat gatcaaaccg atgctggtgg atcagggctg gagcaatgcc
    24301 gagctgggcc aactgaccct gatcagcagc ctggccggga tcgccggagc ctttctcggc
    24361 ggcctgctgt atgcgcgtat cggcgcgttg cgcgcgctac tgctgttcgg tctgttgcag
    24421 gcttgcggga ttgccgccat ggcgctgctg gtaaatgccg gaggcagcac cggcctggtc
    24481 tatctggtgg cgttgttcga gcagaccgcc gatggcatgt ccacggtggc gctgttcgcg
    24541 gtgatgatgc gccagtgccg gcccgagcat gagggggcgg actttaccct gcaggccagt
    24601 gtgcaactgc tactggctgg actggtaggt gccaccagcg gctgggtggc cgagcaactg
    24661 ggttttgcca gtctgtttat cagcgccggt gtgttggggt tgctggctct gcccatcgtg
    24721 ctgctttact tctggcgcgg cgcacggctg gccccccgcg gggcataacg cgccgggtta
    24781 tccgccatgc agtaaaaccc tggtgtttcc tctattccca tctgcgccgg gcgctgcttg
    24841 cccggcaggc gagcaactgg atcaaagatg gctgcctttg cgcctcgtgc gggcaatcct
    24901 gcgtgcctgt ctggattgaa taagaggtgc gtatgcgcaa acccgcgagc ctgagtgtcg
    24961 gcctgcgcct gttgttggcg tcccaggcct tcgccccgtt gagcgtcaag ctggggctga
    25021 ttcagcaact gggggcggag aaggccgcgc tgttggcgcc gcatatgcca cctgggcagt
    25081 tgcgtgagct gatcatggct atgccgattg aatttgctgc ggcggtggcc acccacctcg
    25141 acccacggtt gattctcgat gcctatctga gcctgccgga cagcctgcat ctggaggtag
    25201 cgcgccagct ctgcgccgat ggtgcatttg ccacggcggc actatatgcc gagtgcctgt
    25261 ctgccaaaca gatcaaggtg ctgatctatg gcatcaacga tgtcgagcat gtgctgcaga
    25321 tcgcccggta tatcgttgat atgccactga tcagcgagag cctgcgcagt ttttccaccg
    25381 gttacctgtg caaactcacc gaagcggcgg tgaaggatcg caatctgccg gtggcggcgc
    25441 aggtgctggg tggcttgtcg ttggtgcggc aggcggatgt ctgcgcaggg ttgcagccgg
    25501 caaccctgcg ccaactgctg ccaatgctgc tgttgatcag cggtgatggc ttgcgcaagc
    25561 agctaccgga ggcggtgttg caggtatttg agcagcagtg ggtctaggtg tgctagctgc
    25621 tgggagtcgg ggttattgat cccggcacac aaacgctata tccagcagcg gcacgtcgac
    25681 gatggtcaat gcaaggcaac gggccttagc catatgcatg ggctacgcca tcggctgtta
    25741 gcttttgcag atgagttgcg aaaaccttta acctactggt tgcacagcgt ttgcgagccg
    25801 tctttcctgc ccgaatagcg tggtatagaa acgctgctcg attccgtatc agatggagga
    25861 tgaccgggat tttgactcgg gtgtcttcat taaacttcat gctaagtccc ttgtatttac
    25921 gtcgtgcgac gccatttcgc gctacggcct ttgccggtcg attcgatcaa cccctctgat
    25981 ttcattgccc gcagtaccag ccgcaccatg tcccggctca cgcctgggca ggcttcctcg
    26041 atctccgaaa tcgaaaacgg cagggttcgc cccagagctt ccgcgcgcac ccggtcgcct
    26101 ttgctgcctc gaccgcgctc aatggtgccg acacgctctt caaactcgcg gtaggcccgc
    26161 agcagagctc cccagaagta atcgagccag ggtttgacgt tgtgttgccc ttgatgccag
    26221 ccctgtgagc tggcctccag cgtctcgtaa tagccttcct tggtttcctc gaagatgcgc
    26281 tccaggctga tgtagcggcc cacggcgtag tcgaagcggt agagcagcaa tagggtcagc
    26341 aagcgagaca tgcggccgtt gccgtccggg aaggggtgaa tacataggaa gtcgagcatc
    26401 gccaggggta ccagcaccag tgggtcggcc aggtgctgat ccagtgcggt ggagtagtac
    26461 cgagtcaggt cggccatggc catcggcgtc aaatgagcgg cgactggctg gaatcgcagc
    26521 cgtgacgtgc cgtccggatg gcgttcgatg atgtcgttgt tggtggcttt ccagcgtccg
    26581 ccggcctgcg gcatgtagcg atacagcatg gtgtgcagtt gcagcgccac gccttcgttg
    26641 aaggtcatgt gtgcggccga ttcgtggatc aacgccaggg catcgcggta gccggcgatc
    26701 tcttgctcgg agcgattctt cggtgctgca ttgcggatga ccagagactt cagtcgtgag
    26761 ggcgcgacga caacaccttc caatcggttg gatgactcgg tggattcgac cactgcgatc
    26821 tggcgcaggc ccttgagggc ttcgggagac tgcgcgacgt ataactgttg cttgccctga
    26881 tactcgccca gtgcgcgcag tgtggcggcc tgggtgccat cgaagcgaag cgcagcaagg
    26941 tactcggggg taagcgaacg catggaaatg taagcccagg ctgtttaaat ggggcaatca
    27001 taggctttaa atggggtatt gtctttcaaa atgcctcatt aaagccgttt ggttaccctg
    27061 ttccggcttg ctagctgtat agccccagtg ggttcttggt gcggctattc catccagtgc
    27121 cgttcgtggt gttccagcag gttgctggct tgttccgggc cgtcgctgcc agctgggtaa
    27181 ggacgcgggc tctgatagtg ctggtgccaa cccttgatta ttgggtcgac ccagttccac
    27241 gccgcttcca cttcatcgcg gcgcataaat agcgtcgagt cgccttcgat cacgtcgagc
    27301 agcaggcgct cgtaggcatc ccagcgccgc tgtttgtgga aggcatcggc gaggttgagg
    27361 tcgagttcca ccggttgcag gtgcatgccc ttgcctgggc ttttgcccat cagttgcagg
    27421 ctgatgcgtt cttccggctg caggcggatc agcaggcgat tggcttcgcc gccgccgaat
    27481 agctggtgcg gcaccggctt gaactgaatg aggatttccg aggttttgtg ccccaggcgt
    27541 ttgccggtgc gcaggtagaa cggcacgccg gcccagcgcc agttgtcgat ttcggcctgc
    27601 acggcgacaa aggtttcggt gtcgctgtcg ctgtcgacgt tcttctcgaa gtagtaagcc
    27661 ggcacttcct gaccgccgat cttgccggcg gtgtattggc cgcgaacggt cttgtcctgc
    27721 acgtcgagac cggtgatggg cttcagcgct tcgaggatct tcaccttctc gttgcgcacc
    27781 gcctcggcgt cgaaacgtac cggcgcttcc atggccacca ggcaaagcag ctgcagcagg
    27841 tggttctgaa tcatgtcgcg catggcgccg gctttttcgt agtaggcgcc acggttttcc
    27901 acgccaaggg tttcgcacac gctgatctgc acgtgatcga tatgcccggc gcgccagatt
    27961 ggctcgaaca gcgcgttagc aaagcgcagc gccatcaggt tctgtactgt ctccttgccc
    28021 aggtaatggt cgatgcggaa cacccgcgac tcatcgaaca ccgcgccgat ggcggcgttg
    28081 atcgcctgtg ccgactgcag cgaatggcca atgggttttt ccagcacgat gcgcgccttg
    28141 ggcccggcca ggctggcgac cttcaggtgc gaggcgatgt cttcgaacag gtccggcgcg
    28201 gtggccaggt agtaaatgcg cacccgttca tggtcttcgc ccaggcgctt gcccaggcgg
    28261 ccgaagtcgg cgctctggct ggcgtccatg gcgaagtagt cgaggcgcgc ggcgaagctg
    28321 gcccaggtgt cgttgtcgaa gtcgttgcgc gccacctgag cgcggcagtg tcgctcggcc
    28381 agggcctgaa aggctgtacg gctatgggcg ctgcgcgcca gggcaagaat gcgcgtgtcg
    28441 ccatgcaggc gaccggcgcg atgcaggtga tagagcgctg gcagcagttt atgcagggcc
    28501 aggtcgccgg tgccgccaaa caccagcatg tcgcagggaa tgctcaaggt gggaactccg
    28561 actcggttta gcagtgcggc ggcggcggcc atgtagtata actacaagac cactacaacc
    28621 tgtctttcgc cgatcataac cgagtcatga ctcctatgcg gcgtgctaga tggattcgtt
    28681 agccaaatgt cgagcctaaa cctgtgaacc tgttgcagca cattgcccag tcgcgtcatc
    28741 tacttcgcaa atcggaactc aaggttgccg accatgttct gctcgacccg gcgtcggtca
    28801 tgcacagctc catggccgag ctggcgcaca gcgtcggcat cagcgagccg accatcgtgc
    28861 gtttctgtcg ggcgattggc tgcactggct tccaggacct caagctgaaa ctggcacaga
    28921 gcctggccgc tggcgccagt ttcggccagt tcgcgattca tgaagacgat tccgtggctg
    28981 acttcagcct gaagatcttc gacaccaccc tgcacaccct gattgaggtg cgcgagaagc
    29041 tcgacaccca ggccctgcag caggccattg ccgccatcgc caacgcccag cgcgtggagt
    29101 tctacggttt tggcgcatcc ggcgcagtgg cggcggatgc tcagcacaag ttcttccgcc
    29161 tgctgctcac ggctgcggcc tattccgacc cgcatatgca ggcgatgagt gcggtgaccc
    29221 tcaagcccac cgatgtggcg atctgcatct cgcagtccgg gcgctccaag gatctgctga
    29281 tcaccgccaa cctggtgcgt gaagccggcg cgacgctgat caccctgtgc ccgagccaga
    29341 cgccgctggc cgacctggcc accgtcaacc tggccatcga cgtgcaggaa gacaccgaga
    29401 tctatacccc gctgacctcg cgtatcgccc acttggtggt gatcgacgtg ctcgccatgg
    29461 gcgtagccat ggctcgcggc ccaggcctgg ttaatcacct caagagcgtc aagcgcagtc
    29521 tgcgcagcct gcggctgtcg cctaaggcgc ataagagtag cgacgactga acaccttcct
    29581 ctggtgagcg gtggatgcac aagacgtcat ccactcaccc gactcttgta gcccgaataa
    29641 gcgcagcgca atccgggaag actggccccg gattgcgcca tggcttatcc aggctacgcg
    29701 tacaggctta tggcaccgtt cgtcgggatc accacacttt catcgtcttg cccgcaaagc
    29761 gtcatatccc tggtcgatgc tgtgactccc gttattcagc ttgggagttg caccctatgt
    29821 ctgcttactt ggataaagcc cagaatcacg ccaacagtga ggcccgtgcc cggcgtaaag
    29881 tgattgacca gcgccgcatg gaatatcgtc gtgcgattga aagctatgcc gagcagcgtc
    29941 agctgcagca gcaactggcc gattaccctg aactgattgc ggttaactac ctggcgggca
    30001 agcaggcgtt ggcgcggcaa aacgttcagc cagggcgttg ataaggctgc gttgatcgcg
    30061 cacaaaggcg aaaaacgcct gggccaccgg tgacaggtat ttgccgcgcg ggtgcaccag
    30121 gcaccagctg cgcagcagcg gcagctcact aaccggcagt tcgcgcagcg cgccgcactc
    30181 cagttcgcgg cgcaccgcgt ggcggggcag gagggccagg ccgaggccgg cgatcacgcc
    30241 ttcgcactgc ccttccatcg agccgacttc cagggtctgg gcgaagtgcg cacgcttctg
    30301 gtggcaatac tcctcgcaag cctggcgcgt gccggagccg gcttcgcgca ccagcagcgg
    30361 aaatgccgtg aggtcctgca gtttcaacgc tggctggctg cacagcggat gctcaggcgg
    30421 ggccacggcg atgatcgggt tcgtcagaaa gggcagaaac tccaggttca tgtccgtcgg
    30481 cacccgcgac atgatcagca ggtcatcgcg gttggcgttc aggcgcctga gcgcctggct
    30541 gtgattggtg accaccagct gtaagttgac ttccgggtat tgctggcgaa aagcggcgaa
    30601 caggtgcgga acgaagtact tggcgctcga ttctatcgca aggcccagtt gcccttgcag
    30661 cgagccctgc aggtcggaga gttgcatatc gaggctttcc aggcggccga aaatatccgc
    30721 actggcacgt tgcagggctt cggcggcttc agtcaggtag agcttcttgc cgacgtaatc
    30781 gaacagtggc tggccgatca gttcttccag ctggcgaatc tgcaggctga cggcgggctg
    30841 gctgagtgac atttcctccg ccgcgcggct gtaggagagg tggctgcaca ccgagcggaa
    30901 gatctggatc tgcctgaatg tcatccgtag cagcgatttg cgcatggtcc ctccctgtct
    30961 agcgtggtct gagcgcacga ctataagtct ttacttatat gtaacccaat aaatattgat
    31021 tttggttaat caccaagccg gcgtagctta aatgcctgac cgctacatga cgtgcggtca
    31081 cacgtcgacc ggtattgccg ttcgcctgct cacgtgatcg aggacgctgg ctcgtgatca
    31141 agaaaatcct gatagccaac cgcggtgaga ttgccgtacg catcgtgcgg gcctgtgccg
    31201 aaatgggcat tcgctcggtg gcggtgtact ccgatgccga ccgccacgct ttgcacgtca
    31261 agcgtgctga cgaggcgcat agcatcggtg aggagccact ggccggctat ttgaacccgc
    31321 gcaagctggt caacctggcg gtggaaaccg gttgtgatgc gctgcatccc ggttatggct
    31381 tcctctccga gaacgccgag ctggcggata tctgtgtcga acgtgggatc aagtttatcg
    31441 gcccgtcggc ggaagtgatt cgccgcatgg gcgacaagac cgaagcgcgg cgcagcatga
    31501 tcgccgctgg cgtaccctgc acccccggca ccgaaggcaa cgtggcggat atcgccgaag
    31561 cactgcgcga aggcgaccgc atcggctatc cggtgatgct caaggccacc aacggcggcg
    31621 gtgggcgcgg tatccgccgc tgcaacagcc gcgacgagct tgagcaggcc tatccgcggg
    31681 tgatctccga ggcgaccaag gcctttggcc gcgccgaggt gtttctggaa aaatgcatcg
    31741 tcaatcccaa gcacatcgag gcgcaggtcc ttgctgacag cttcggcaac accgtgcatc
    31801 tgttcgagcg cgattgctcg atccagcgcc gcaatcagaa gctcatcgag attgccccca
    31861 gcccgcagct gacccctgag cagcgcgcct atatcggtga cctgtcggtg cgcgcggcca
    31921 aggccgtcgg ttacgagaac gccggcaccg tggagtttct gctggccgag gatggcgagc
    31981 tgtactttat ggagatgaat acccgggtgc aggtggaaca caccatcacc gaggaaatca
    32041 ccggtatcga catcgtccgt gagcagatcc gcatcgcctc cggcctggag ctgtcggtca
    32101 agcaggaaga catcatccac cgtggcttcg ccctgcagtt tcgcatcaac gctgaagacc
    32161 cgaagaacaa cttcctgccc tcgttcggca agatcacccg ctactacgcc cccggcggcc
    32221 ccggcgtgcg caccgacacg gcgatctaca ccggctacac cattccgccg tactacgact
    32281 ccatgtgcct gaagttggtg gtttgggcac tgacctggga agaggcgatg gatcgcggcc
    32341 tgcgtgccct ggatgacatg cgcgtgcagg gggtgaaaac caccgcggcc tattaccagg
    32401 aaattctgcg taacccggaa tttcgcagcg gccagttcaa taccagtttt gtcgaggcgc
    32461 acccggaatt gaccgagtac tcgatcaaac gcaacccgtc gcacctggcc atcgccatcg
    32521 ccaccgccat tgctgcccac gccggcctgt aggggaagga ttcagatcat gagtaagacc
    32581 aagaaaatca ctgtcaccga taccatcctg cgcgatgccc accagtcgat cattgccacg
    32641 cgcatgcgcc tggacgatat gctgccgatc tgcggcaagc tcgataaagt cggctactgg
    32701 tcgctggaag tctggggcgg cgcgaccttc gattcctgcg tgcgcttctt gaaggaagac
    32761 ccctgggaac gcctgcgcaa actcaaggcc ggcctgccca acacccgtct gcagatgctg
    32821 ttgcgcgggc agaatctgct cggttatcgc cactacagcg atgacgtggt gcgtgccttt
    32881 gtggccaagg cggcggtcaa cggtatcgac gtgttccgca tcttcgatgc gatgaacgat
    32941 gtgcgtaacc tgcgtgtgtc catcgaagcg gtgaaggctg ccggcaaaca tgcccagggc
    33001 accatcgcct acaccaccag cccggtgcac accgtcgagg cattcgtgaa gcaggccaag
    33061 gccatgcaag cgatgggcat cgactccatc gcgatcaagg acatggctgg cctgctgacg
    33121 ccgtacgcca ccgctgatct ggtgcgtgca ctgaaggctg aggtcgactt gccggtattc
    33181 atccatagcc acgacaccgc cggcatgggc tcaatgtgtc agctcaaggc gattgaagcc
    33241 ggcgccgacc acatcgacac cgccatctcc agcttcgcct ggggcaccag ccatccgggc
    33301 accgagtcga tggtcgccgc gctcaagggc agcgaatacg acaccggcct ggacctggcg
    33361 ctgatccagg agatcggcct gtacttccac gccgtgcgca agaagtacca ccagttcgaa
    33421 agcgagttca ccgccgtgga cacccgcgtg caggtcaacc aggtgccggg cgggatgatg
    33481 tccaacctgg ccaaccagct gaaagagcag ggcgcgctga accgcatcaa cgaagtattc
    33541 gcggaaatcc cgcgggtgcg cgaagacctt ggcttcccgc cgctggtcac gccgacttcg
    33601 cagattgtcg gcacccaggc agtgttcaac gtgctcgccg gtgagcgtta caaaaccatc
    33661 accaacgaag tgaaacttta cctgcaaggc cgctacggcc tggcgccggg caagatcaac
    33721 gaacaactgc gcaagcaggc gattggcagc gaagaggtga ttgaagggcg cccagctgat
    33781 ctgctcaagc cggaaatggc caagttgcgc gaggagatcg gtgcgctggc caagtccgaa
    33841 gaagacgtgc tgacctacgc catgttcccc gatatcgggc gtaagttcct cgaagaacgc
    33901 gctgccggca ccttgactcc cgaagtgttg ctgccaattc ccgaagccgg tggcatggcc
    33961 ccggcaggtg gcgaaggtgt gcccactgag tttgtagtgg acgtgcacgg cgaaagctac
    34021 cgcgtggata tcaccggcgt tggcgtcaag aatgatggta agcgccactt ctacctctcc
    34081 atcgacggta tgccagaaga ggtggtgttt gaaccgctga atgatttcgt cgcgggtagc
    34141 tcaggcaagc gcaagcaggc cagcgcgccg ggcgatgtca gcaccagcat gccgggcaat
    34201 atcgttgatg tgttggtcaa ggtcggcgac acggtcaagg ccggccaggc cgtgctgatc
    34261 agcgaagcga tgaagatgga aactgaagtg caggcgccga ttgccggcac cgtgaaagcg
    34321 gtgcatgtgg ccaagggcga ccgggtcaat ccgggtgacg tactggtgga aatcgaaggt
    34381 taacctcgga agccccggct gtttgaacag gcatttgaat ggtttttttg gggcttcttt
    34441 ttacatcgcc ggcgcggctt tttagccgcg ctagcatggg tttttaggcc aagtgcctga
    34501 gtcccctagg agccgtacgg ctcctttttt tcggttatgc cccgctgtgc agggttctgc
    34561 tgttctcagc aggagactgg gcaaaactgc gggtatcagc acaacgcgtg gtgggggcat
    34621 gaccttttat ttgagcaggt gtgacgcggc aggcatctca atagcagttc aatacaggag
    34681 caatgcgcgg tgagtttgga tccggtggtg ctgttctttg tcttcggtct gttggcgggg
    34741 ttgctgaaaa gcgagctgaa actgccgcct gcgctgtatg aaaccctatc catcattctg
    34801 ctgctggcca ttggtctgca cggcggcgtg gaactggccg agcaggccag ctggcagttg
    34861 ctcggtcagg cagcctgggt gctgggcctg ggtgtggcgt tgccgctact ggcttttgcc
    34921 gtgctgcgcg gcctgggctt cgaccgggtc aacgccgcgg cagtggctgc gcactatggc
    34981 tcggtcagcg ccggtacctt cgccgtggtg gtggcttaca tgctggccaa gggcatcgag
    35041 tttgaaagct atatgccgct gtttgtcgcc attctggaaa tcccggcgat cctggttggc
    35101 attctgttgg ccaagggtat tgctcgcgat accgattggg gcgagctggg ccgggagatt
    35161 ttcctcggca agagcatcat gctactgctc ggtggcctga tcatcggtgc gattgccggc
    35221 aaggaggcga tcaagccgct tgagccgttc tacaccagca tgttcaaacc ggtgctggcg
    35281 ctgttcctgt tggagatggg cctgatcgcc tccggccagc tcggctcgct caggcagttt
    35341 ggcctgcgcc tgcttggctt tgccctgctg atgccgctgt tgggcgcgct gatcggcgcg
    35401 ttgctgggcc ggtttatggg gctgagcttg ggcggcacgg cggtgctggc cacgctagcg
    35461 gcgtcggctt cttatatcgc cgtgcctgcg gccttgcgtc tggcattgcc cgaggccaac
    35521 ccgtcgctgt cgctgacggc atcgctgggc atcacttttc ccttcaatat catcgtcggc
    35581 atcccgttgt acctgatgct ggccgaacag ctgctggcct gggggctctg aaatggacgc
    35641 gcatatccgc accctgctca ccgtgatctg cgaagctgca ctggaaaaga aactggtggc
    35701 tgacctggag cagctcggcg cgccgggctg gaccatctcc gacgcccgtg gccgtggtgg
    35761 acgcggcgtg cgcagcgccg gctgggacac cgaaggcaat atccgcgtgg agatcatctg
    35821 cacccgcgat gtcgccgaac gcatcgccgc ccatctgcag gcgcgctact acgccaactt
    35881 cgcgatggtc tgctacctgg ctcaggtgga agtgttgcgc gcggaaaaat tctagccggc
    35941 aaccgaacag ccccggatta cgccaaggct tatccgggct acggtgatga gtagcccgaa
    36001 caagcgctag cgcagtccgg gattgtggtt gggcgacggg tgatgccagc gcgcactcat
    36061 ccggctggtt cagtgtggcg cttcccggat tgcgccaagg cttatctggg ctacggcgag
    36121 gagtagcccg aacaagcgtt agcgcagtcc ggggcattac caacaaggag gttggcatgg
    36181 tcaattaccg gcgcgcgcgc gtcgcgggtg gcacctattt cttcacgttg accttaaaga
    36241 atcggcgtag tgatctgctg gtgcggcata ttgatttgtt acgtgacgct ttgcgccaag
    36301 tgaagcggcg gcggccattt acgttattgg ccgtggtggt tatgcccgac cacgtgcacc
    36361 tgttgttgca attgcccgat atggatacca actactcggc tcgtttgcgt gatttcaaaa
    36421 tgctgttcgt caaggcacta cgcgccgctg gcgggcccat tcggtcggat gtgtggggtg
    36481 aggcgggtat ctggcaagcg cgtttctggg agcatacgat ccgtgatgag cgcgatcttg
    36541 cggcccatat cgattacatc cacttcaatc cggtcaagca tggtcatgtg tcgcgggcag
    36601 tggactggcc ctattccagc ttccatcgtt atgtgcgcca aggtctattg tccgccgatt
    36661 gggctggcgg tgcagggctt gagttggccg gtgacgattg aatcgcttcc cggattgcgc
    36721 caaggcttat ccgggctacg gcgtgggata gcccggataa gcgtcagcgc agtccgggat
    36781 tgattggtat tgggtgtgat cagcgccgtc aggcaagcgt ctacctgcca tcgtccgagg
    36841 tgcgctcggg ccttggtggc gagcctaagc atgcaagcgc tgttgaatga aattctggat
    36901 caagtccgcc cgctgatcgg gcagggcaag gtggccgatt acatcccggc gctggccgat
    36961 gtgccggcca accagctggg tattgcggtg tacagcaacg acggcgagct gtactgcgcc
    37021 ggtgatgcgg acacggcgtt ttccgtgcag agcatttcca aggtgttcag cctggtgcag
    37081 gcgattcagc attccggtga ggatatctgg cagcgcctgg gccatgagcc gtctggtcag
    37141 ccgttcaatt cgctggtgca gctggaattc gagcgtggcc gcccgcgcaa tccgtttatc
    37201 aacgccgggg ccctggtgat ctgcgatatc aaccaatcgc gctttgccgc gccggtgttg
    37261 tcgatgcgcg attttgtccg gcgcctggcc ggaaacccgc agatcgtggt ggatcggcgc
    37321 gtggcggatt cggaatatca gcaccgcgcg cgtaatgcgg cggcggccta tctaatgcag
    37381 tcgttcggta atttccacaa cgatgtggaa gcagtgctgc gtagctactt cagctactgc
    37441 gcgctgcgca tgagctgcgc cgacctggcg cgggcgttct gctttctggc caacgaaggc
    37501 ttttgcaaac acagcggtgc gcagattatc agcgcgcggc agagcaagca ggtcaacgcg
    37561 atcatggcta ctagcggcct gtatgacgag gccggcaatt tcgcttaccg cgtcggtttg
    37621 cccggtaaaa gtggcgtcgg cggcgggatt attgccgtgg tgccggggca gtttagcgtg
    37681 tgcgtctggt cgccggagct gaacgccgcc ggtaactcct tggccgggat caaggccctg
    37741 gaactgttaa gcgagcggat cggctggtcg gtgttctgat tcgtgccgtg ggaacggcgg
    37801 gaacaagtgc catgcccgcg aaacgcggtc atgggcatgg gttaaatacc tccggcgctc
    37861 gtataaaaaa taacccattg ttttatatag aaattaaatc gaaagaaaaa gtatgtagga
    37921 gccagcttgc tggcgatctt tgtgggtcaa aagcatcgcc agcaagctgg ctcctacaag
    37981 gtgaaatgca tcacccggca atcagctcct tggccgcctg gcggtgatcg gcgattagct
    38041 tggccacatc cagaccttcg atctgcccgt cgatgactcg ccactggccg ccaatcatca
    38101 tccggtcggc cttgtcggca ccgcacagca gcagcgcgga aatcgggtcg tggctgccgg
    38161 agaagcgcag ctcgtcgagc ttgaagaagg ccaggtcggc ctgcttgccc actgccagtt
    38221 cgccgatatc gctgcgcccc agcagtttag ccgaaccctt gctggcccag cccaacacca
    38281 attccggggt gatcttctcg gcgccgtagc gcaggcgctg gatatacagg gcctggcgcg
    38341 cttcgaggat catgttcgag gcatcgttgg aggccgagcc atccacgccg aggccgatcg
    38401 gtgcgccggc ggcttgcagg tcgagggtcg ggcagatgcc cgaggccagg cgcatattcg
    38461 agcttgggca gtggcagata ccggtgccgg cctggcccag gcgggcgatt tcatccgggt
    38521 tgaagtgaat gccgtgggcc agccaggtgc gcgagctgag ccagccgacg ctgtccagat
    38581 agtccacggt gcgcaggccg aagcgctgca ggcagaagtc ttcttcgtcc agggtctcgg
    38641 ccaggtgggt gtgcaggcgc acgtccagtt cttcggccag ttctgcgctc tggcgcatga
    38701 tttcctgggt cactgaaaac ggcgagcagg gcgccagggc gatctggatc tgcgcgccgt
    38761 cgccgcgctg gtggtagtcc tggatcagcc gttggctgtc agccaggatc acctcgccct
    38821 gctgcaccgt ctgctgcggc ggcaggccgc catcggcctg gcccaggctc atcgagccgc
    38881 gggtgagcat ggcgcgcatg cccagctcgc gcaccgcctc gacctgcacg tcgatggcgt
    38941 tttccaggcc gccggggaac aggtagtgat gatccgccgc ggtgctgcag ccggacagca
    39001 gcagttcggc cagggccacc ttgctggcca gcgcgagctt ctcgggggtc aggcgtgccc
    39061 acaccgggta gagggtctgc agccagggaa acagcggctg attgaccacc ggagcccagg
    39121 cgcgggtgag ggtctgatag aagtggtggt gggtgttgat cagccccggc agcaccacat
    39181 gctcgcgggc atcgaagctc tgctcgcagg gctgggacgg ttgttgaccg gccttgagca
    39241 gttcgctgat cacgccgttt tcaatcacca ggccgccggc ggcgtcgagg ttgttggcag
    39301 tgaagatggc tagggggttt ttgatccaga tacgtgtagc agccattgag ttggctcctc
    39361 tgaagttggg ttcaggttag ccagctcagt gtttaccctg tctgctgatc caggtgcgcg
    39421 gccggcgcag gtggccgggc gtggcgcaaa agataacgcc gtaatctgcc tgcggcaaga
    39481 gcgcatggca gaatttctga ccacgaagtc agtgcttaga acactttgcg gtattggatg
    39541 aaaccagagc gttcagcgat gcgttcatac agcagcatgg cgcggctgtt gctctcgtgg
    39601 gtcagccagt aggtgcgtga gcagccctgg gcctgggctt cttggtaaac gtgctcaatc
    39661 agcttgcgcc ctgcgccgct actgcgcaca tccttggcga taaacagatc ctgcaggtag
    39721 cagtagtcgc caggggtcca ggtcgagcgg tggtagatcc agtgcaccag gccaatcgcc
    39781 tggccttcat gccaggccag cgccgcgtgc atcggctcgg cagggtcgag gaagcgctgc
    39841 caggtaatgt cgctggtcgc ggcggcaatc tcggtcatat agaaacgctg atagccttgc
    39901 cacagcggca gccaggcagc gtgatcagcg tcgctgatcg ggcggatttc gacgggaatg
    39961 cgaatcggca tggcattggt tcctcgatgg ggtaaagccc ttacagccta gccgtaaggg
    40021 cgcaggcttc aatctgcgcg ttcaccacag acatccaggg cgtaccagtg ttccacctcg
    40081 gcctggctca ggccggcgcc gagcagggcg aacagcttgc ccagagccgc ttcacgggtc
    40141 atgccgccgg cggaaatcag cccggcactg gccagctgac tgccggcggc atacacgccg
    40201 aattcgacat ggccgtgcgg gcactggctg atcgcggcca gcaccacacc acgttcatgg
    40261 gcggccttga gtacggcgag cagttcggcg tcatccgcag gcccggtgcc gctgccgtag
    40321 cattccagca gcagaccttg tacgccactg tccaacaggg cgcgcacgtg gcttgcctgg
    40381 atgcccgggt aaaacggcag cacggccaga ttgaccggct ggcgtggctg gcgatagtcg
    40441 atgctcgccg gaatgctctc ggcacgtggg ctctggcgct gacgagggag cacgtggaag
    40501 gcgtcgaagg catcactgcg cagcttgctg acccgcgcgc catgcagcag ctcaccgttg
    40561 aaatacaggt gcacgcccgg cgtgacgccg gcttgcagtg cgcgcatggc accaaacagg
    40621 ttactccagg catcgctgcc ctcggcaccg gccggcagca tcgagccgct gaggattacc
    40681 gggactggca aacccagcag cagaaagctc agggcggcgg cgctgtacgc cagggtgtcg
    40741 gtgccgtgca gcagcagtac accgtcgcag ccgtcctgct caataccggc gcgaatcgct
    40801 gcgaccatcg ccagccagtt gctctggttc atattggcgc tgtcgatggg cggcagcagc
    40861 tcgcggaaga tccactcagg cagtggctgg gcttcgcggc tctgctgagc gcgcaggcgc
    40921 gcttcgaaac cggacgccgg agccaggccc tcggcgctca tctgcatgcc gatggtgccg
    40981 ccggtataaa gcaccaggag ttttttacaa cccatagggg ttctccgttt tttcattgtt
    41041 cttataagca aacagggggc taagccccct gtttatcaca accctttcag catcttagcg
    41101 ctggtgtacc gcgttaccga tttccgcctg ggttgccggg gcgctgtgcg ccgcgtttgg
    41161 ccaggccgct gggtcgagat ccaggtcagc gaagttcttc gcatcgaaca ccggcgattc
    41221 cacaccggcc ttgatctggc tgtcgtagtc gcgcatcaga cgcaggccga ccttgaacag
    41281 cagtaccagg gcgatcaggt tgcagatggc cagcagaccc atggtcacgt cggcaaaggc
    41341 gaacaccgta ccgagatcct gtaccgagcc ccagaggacc agcgccacta ccagcacgcg
    41401 gtaaaccacc accgggccgc gtttcttgct gaagaagccc agggcgtttt cgccgaggta
    41461 gtagttgtag atcagggtgg tgaacacaaa cagcagcagc gccacggtaa cgaacacccg
    41521 gccccactca ccgaccacgc tggcgacagc ggtctgagtc agcactacgc cggccatctc
    41581 ggtgcctggc tgatacacgc ccgacagcaa gatgatcagc gcggtgcagc tgcacaggat
    41641 aatggtgtcg ataaatacgc tgagcgactg cacgatgccc tgagcgactg gatgcttgac
    41701 ctcggcaacg gcggcgacgt tcggcgcact gcccagacct gcttcgttgg agaacaggcc
    41761 gcgttttacg cccatcagga ttgccgcacc gataccgccg gcaaatgctg gctcgagacc
    41821 gaaagcgctc ttgaagatca gcgccagggt ggctgggatc gcatcgacgt tgctgccgat
    41881 cacgaacagc gccatggcga tgtaggagaa ggccatcact ggcaccagca cgtcagcaaa
    41941 cttggcgatg cgcttgatgc caccgaagat gatcaggccc attaccacgg tcagaatcgc
    42001 gccgctggca taggtcggca gaccgaaggt gtcatgcatc gagctggcca cggtgaacga
    42061 ctgcacggcg ttgaagccga agccgaacgt caccagcagc agaatcgaca ccacaatgcc
    42121 caaccagcgc tggcccaggc cgtgctgaat gtagaaggcc gggccaccgc ggtaggtgcc
    42181 gtcgtcttca cggcgcttgt acagctgcgc cagggaacat tcgaagtagc tggtggccat
    42241 gccgaccaac gctacgaccc acatccagaa gatcgcgccc ggaccaccga gcatgatcgc
    42301 cacagagaca ccggcgatgt tgccggcacc gacgcggccg gctaccgaga gcatcagcgc
    42361 ctggaacgag ctgagctggc cgggctggcg ctggaaggct tcggcgaaga tacggaacat
    42421 gctgccgaag taacggaact ggacgaaacg cgaggcgatg gtgaagtaca gaccaaggcc
    42481 gatcagcatc acgatcagga gcttgctcca gatcaggtcg ttgagaatat cgagcatggc
    42541 agtgaggtct ctcttg
//
LOCUS       DASRUM010000211         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1292660, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000211 DASRUM010000000
VERSION     DASRUM010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292660"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="VFF22_14170"
     CDS             complement(<1..178)
                     /locus_tag="VFF22_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006414895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6499 domain-containing protein"
                     /protein_id="HZX17991.1"
                     /translation="MAELSSDHWHPSAAYLYVLHLDGPALAWEYLRRDPDYQHDWLRR
                     QRYPEVAQRWGLRLL"
     gene            complement(540..890)
                     /locus_tag="VFF22_14175"
     CDS             complement(540..890)
                     /locus_tag="VFF22_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2958 domain-containing protein"
                     /protein_id="HZX17992.1"
                     /translation="MTQPLITDEQRAQLLANGRASAEGLDIDPSPVVKLFTPDAHATW
                     LLTELDPEDGDTAFGLCDVGIGMPELGNVRISELASIVGPLKLPIERDLYFVAKRTLS
                     DYARLARINGSIID"
     gene            1333..1623
                     /locus_tag="VFF22_14180"
     CDS             1333..1623
                     /locus_tag="VFF22_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006452450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZX17993.1"
                     /translation="MQKRSIQRGRPVGATTFEAEPAQAFGAAVRALRTEQGVAQETLA
                     HLAGIERSHMGKIERGEHMPTLAIILKVARALGCSAADLMTATESRLAAAKS"
     gene            complement(1678..1986)
                     /locus_tag="VFF22_14185"
     CDS             complement(1678..1986)
                     /locus_tag="VFF22_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 domain-containing protein"
                     /protein_id="HZX17994.1"
                     /translation="MANIGTFTADKDGFTGTLRTLTLNVKVKFIQNDKTSENAPDYRI
                     QAVNGLEVGAAWKKISQAERPYMSCSLDDPSFPATIYARLIEGEDGTHNLIWSRSKGD
                     "
BASE COUNT          514 a          713 c          848 g          454 t
ORIGIN      
        1 ccagcaggcg taggccccag cgttgcgcca cctcggggta acgctgacgg cgtagccagt
       61 cgtgctggta atccgggtcg cgccgcaggt attcccaagc caaggctggg ccgtccaggt
      121 gcaggacgta gagatacgcg gcgctcggat gccagtgatc ggaactcagc tctgccatgg
      181 cgcaacctcc tgtcggatag caggaacagt cgccacgggc atcggtgcta cgaagctatg
      241 gagtcatcgt caaaacgtca tggggcattt aataaactgg cagtgtcaag cccaatggac
      301 tccgaagcgt tgcagggggt gtccgaatgc gacggctacg caaccggaaa tggtgcgcag
      361 tatccgacca cggtgccgca aggcccgcca ggcatcgtga ctgtttgggt cgcgtttgca
      421 ccgctgcggt gcatagtctg gattgatgcg atcaaagtcc gaactgagac cgaaatagac
      481 acagtacgcc ggaggcggca gcgcctggat ggcccgccgc agcgggccat gcggttcgat
      541 cagtcgatga tcgaaccgtt gatgcgggcc agccgcgcat agtctgacaa ggtgcgcttc
      601 gcgacgaaat atagatcccg ctcgatgggc agcttcagcg gcccgacgat ggatgccagc
      661 tcggatatcc gcacgttgcc cagctcgggc atgccgatgc cgacatcaca caggccaaag
      721 gccgtatcgc catcctcggg atcgagttcg gtgagcagcc aggtcgcgtg cgcgtccggg
      781 gtgaacagct tgacgacggg cgacgggtca atgtccaggc cttcggcact ggctcggcca
      841 ttggcgagca gttgggcgcg ttgttcgtcg gtgatgagag gttgggtcat ggtcgcttcc
      901 tttgaagggt tcactccacg gcggagcgag gcgactgggg cgcacgcgcc aagcgcagcc
      961 gtcatacccc aacacggtcc ggtgcgggcc ggggttgacg gcaagcggcg tgcgccagac
     1021 tcgcgtactt gtccgctgtg gagtgaaccc gagagcggac aggatgccca caaagccgac
     1081 gacgcacgaa gccgtattgc tggatgcacg gaaagcctca gatgtgccat taaggattta
     1141 cggaagatcg taaaagcgta cagatggaag tcaacgaatc cacggaagca cagaaacacg
     1201 aatccggctt tacgcagatg cgtaaaagga cgatgacgag cgagataccg ttacccaata
     1261 tggtaggccg aaatagccaa tgagttaaag ataacgtggt tttaattgtg ctgttaatcg
     1321 acgcttctgt gcatgcagaa gcgatcaatc cagcgtggcc gcccggtcgg cgccaccact
     1381 ttcgaggccg aaccggcaca agccttcggc gcggcagtgc gcgcgctgag aacggagcaa
     1441 ggggtcgccc aggagacgct ggcccatctg gcgggcatcg aacgctcgca catgggcaag
     1501 attgagcgcg gcgaacacat gcccacgctg gcgatcattc tcaaggtcgc aagggcgctg
     1561 ggttgcagtg cagccgactt gatgacggcg acggaaagcc ggcttgcggc tgccaagtcg
     1621 tagcactggc cgtcagataa caccccgcca cggtgggcgg ggcatcgagg gcggcgatca
     1681 gtcgcccttg ctacgcgacc agatcaggtt gtgcgtgccg tcctcgcctt cgatcaggcg
     1741 agcgtagatc gtggccggga acgaagggtc gtccaagctg catgacatgt agggccgctc
     1801 ggcctggctg attttcttcc acgccgcgcc gacttccagc ccgttgactg cctggatgcg
     1861 gtagtcgggg gcgttctcgc tggtcttgtc gttctgaatg aacttgacct tgacgttaag
     1921 cgtcagggtg cgaagcgtgc cggtgaagcc gtctttgtct gcggtgaagg tgccgatgtt
     1981 agccatgatg aagtttcctt tcggggttgc aagatcgcgc ccatcgcgtc cttgtcggtg
     2041 atccaagggt gagggtcggg cgggctgcac cgcgcagcgg ccgcaacagc gtggaggacc
     2101 gggaggccca aagaatttgt tgcgcgagga aggcgcacag cgccagggaa attgtttggg
     2161 ccagacggtt gcagccatga agcccgaggc gtaagccgtg cccttgccag gattcacgac
     2221 aagccaagga cgcaggggtc gaactggtcc gaatggagcg atggctaact cggcattccc
     2281 gcaggaaggc agcgccggca tgcgccttga tgtcggtaag gtctggctgg gaatgactgg
     2341 cgggaacgct cccagcaccg gacggcaacg gattgaggcg tggcgtggaa tcttcatggc
     2401 aaggctgtgc ggtgcgtcgt tgaagcgtct ttcgtgacgt gtaacggaat tccctccagc
     2461 gtgctggatg gcacgcattc gcacaacctt gcgcagcaag gtgcagcgtg ccccatcgcc
     2521 ccgcccacc
//
LOCUS       DASRUM010000212        19871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2274405, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000212 DASRUM010000000
VERSION     DASRUM010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 19871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19871
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2274405"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="VFF22_14190"
     CDS             <1..1486
                     /locus_tag="VFF22_14190"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020305015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HZX17995.1"
                     /translation="EQPAESTKGFTLAQKMVGRACGVTGVRPGTYCEPKMTTVGSQDT
                     TGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPIDVTTHHTLPDFIRTRGGVSLRP
                     GDGIIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESV
                     LVRFKGKLQPGITLRDLVHAIPYVAIQKGLLTVEKKGKKNIFSGRILEIEGLDELTVE
                     QAFELSDASAERSAAGCTIKLPEKAIAEYLRSNITLLRWMISEGYGDARTMERRAQAM
                     EAWLADPKLMEADKDAEYAEIIEIDLADINEPVLCAPNDPDDARLLSSVQGEKIDEVF
                     IGSCMTNIGHFRAAGKLLEKVEGTLPTRLWLSPPTKMDQHQLTEEGYYGIYGKAGARM
                     EMPGCSLCMGNQARVEANSTVVSTSTRNFPNRLGDGANVYLASAELAAVAAITGKLPT
                     VEEYMVYAKKIDTMAADVYRYLSFDQIAEFREAAANAQIPAVQV"
     gene            1702..2898
                     /locus_tag="VFF22_14195"
     CDS             1702..2898
                     /locus_tag="VFF22_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003183596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HZX17996.1"
                     /translation="MNEHDISRSDTPEKPVLNIGFMALTDAASVIVAATQGFAEKHGL
                     TLNLQRQASWATLRDKLLSGELDAAHSLYGLIYGVQLGLGGAPATDMAVLMGLNQNGQ
                     SINLSPALQAAGVSTGSALAQRAHQSGAKLTFAQTFPTGTHAMWLNYWLASQGIHPLS
                     DVDSVVVPPPQMLAHLKARRIDGFCVCEPWSAQAVDEQMGCTLTTSQAIWPDHPEKVL
                     GCTREFAEQHPNSARALIMAVLEASRFIDESEENQRSTAQLLSGSEYVDAPLSAIQPR
                     FLGQYQDGLGHAWLDAHPLRFHADGAVNQPYLSDGMWFMTQFRRWGLLREDPDYLAIA
                     RQVQQLDLYRQAADALGIAVPSEMRSSQLLDGQVWDGSDPAGYARSFALHALADASIG
                     AQQESR"
     gene            2900..3478
                     /locus_tag="VFF22_14200"
     CDS             2900..3478
                     /locus_tag="VFF22_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="HZX17997.1"
                     /translation="MLRILLINDTAKKVGRLKTALTEAGFEVIDESGLTIDLPERVEA
                     VRPDVILIDTESPSRDVMEQVVLVSRDQPRPIVMFTDEHDPGVMRQAIQSGVSAYIVE
                     GIHAQRLQPILDVAMARFESDQALRAQLQARDAQLAERKRIELAKGLLMKMKSCDEEQ
                     AYTLMRRQAMSRQQKLAQVAEQIIAMHDMLGG"
     gene            3661..4887
                     /gene="alaC"
                     /locus_tag="VFF22_14205"
     CDS             3661..4887
                     /gene="alaC"
                     /locus_tag="VFF22_14205"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HZX17998.1"
                     /translation="MADQASRRFARIDRLPPYVFNITAELKMAARRRGEDIIDFSMGN
                     PDGPTPPHIVEKLVQVAQREDTHGYSTSRGIPRLRRAISRWYKDRYEVEIDPESEAIV
                     TIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLIPGVDFFAEL
                     ERAIRESIPKPKMMILGFPSNPTAQCVELDFFERVVALAKQYDVLVIHDLAYADIVYD
                     GWKAPSIMQVPGAKDIAVEFFTLSKSYNMAGWRIGFMVCNKELVSALARIKSYHDYGT
                     FTPLQVAAIAALEGDQQCVRDIAEQYRQRRNLLVKGLHELGWMVENPKASMYVWAKIP
                     EQYAALGSLEFAKKLLLEAKVCVSPGIGFGDYGDDHVRFALIENQDRIRQAIRGIKTM
                     FRADGLLPGKKAKADE"
     gene            complement(4923..5315)
                     /locus_tag="VFF22_14210"
     CDS             complement(4923..5315)
                     /locus_tag="VFF22_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX17999.1"
                     /translation="MQQMLMTIVLLGLLAGCAQPQLDQPRANGTYLVIENGQAWAVLV
                     SDGKRVEEQGTVVDVIRLPSENSAVAASYVIETANCGRVQWLSERSDAADGMTNLMSL
                     HSNNQLGQSGCVITSGLSKVWTVLDYSG"
     gene            5528..5845
                     /locus_tag="VFF22_14215"
     CDS             5528..5845
                     /locus_tag="VFF22_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZX18000.1"
                     /translation="MLDVLQLKHSVNRMPLEKVRATVNELQLEGIVTEGKTPFSRVHF
                     NTCFAEIEALLQRAGYHRQLDVVGYQGLAYAMFDPSRWEAVEVLRWLKEFVEEAQGKT
                     LSR"
     gene            6289..7677
                     /locus_tag="VFF22_14220"
     CDS             6289..7677
                     /locus_tag="VFF22_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HZX18001.1"
                     /translation="MRNSTARRSLLKKTLAGLGCAAVMLLSPLSVQITLAAEAEKDEL
                     KFGFIKLTDMAPLAIAYEKGFFEDEGLYVTLEAQANWKVLLDRVIDGELDGAHMLSGQ
                     PIGATIGFGTKADVVTAFTMDLNGNAITVSNAVWEEMKKHVPMEGGKPVHPIKADALK
                     PVIDKFNAGGKSFNLGMVFPVSTHNYELRYWLAAGGIHPGYYAPHKGDTSGQIDAKAL
                     LSVTPPPQMPATMEAGTIHGYSVGEPWNQAAVFKGIGVPVVADYAIRKNMSEKVFGVS
                     KSWAEANPETHKRVVKALIRAGQWLDADNNANRQEAVEILSRPEYVGADAKVIANSMT
                     GTFEFEKGDKREVPDFNVFFRYHATYPYYSDAVWYLTQMRRWGQIAETKPDSWYLETA
                     KKVYLPAIYREAAAELIAEGKFAASDFPPADDDGFRAPLTDTIDAVPYDGRKPNAYLE
                     QFEIGLKGDTQL"
     gene            7744..8736
                     /locus_tag="VFF22_14225"
     CDS             7744..8736
                     /locus_tag="VFF22_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX18002.1"
                     /translation="MSNPSVATPITATLKSARSSALLKRWAPALILPMLGIIGFLLCW
                     QLLANNIQTSLGQFPGPVQVSEQFSGLVSAHFKEREKARVFYERQDTRNAALLAKNPD
                     AEIKVRPYTGKPTFIQQIFTSLYTVMAGFVIASLIAIPLGIVCGLSKSIYTAINPLIQ
                     VFKPVSPLAWLPLVTMLVSALYVTDDPLFAKSFLTSAITVALCCLWPTVINTTVGVAS
                     IEQDLNNVSKVLSLSPLAHVRRIVLPAAVPMIFTGLRMSLGTGWMVLIAAEMLAQNPG
                     LGKFIWDEFQNGSSNSLGRIMVAVIVIGLIGFALDRLMLLLQRQFSWDKNAVLR"
     gene            8751..9545
                     /locus_tag="VFF22_14230"
     CDS             8751..9545
                     /locus_tag="VFF22_14230"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX18003.1"
                     /translation="MSHSHLELTGVGIDFPTDKGLYRALQNVNLRIDKGEFVSLIGHS
                     GCGKSTVLNIVAGLYKATTGGVILDGREVDEPGPERAVVFQNHSLLPWLSCYQNVELA
                     VRQVFQGKKSRSEMRDWIEHNLQLVHMMHAKDKRPSEISGGMKQRIGIARALAMQPKV
                     LLMDEPFGALDALTRAHLQDSLMEIHADLGNTVIMITHDVDEAVLLSDRIVMMSNGPA
                     ASVGEILQVQLQRPRDRIALAHDARYHEYRAAVLEFLYQKQQRPAA"
     gene            9682..10371
                     /locus_tag="VFF22_14235"
     CDS             9682..10371
                     /locus_tag="VFF22_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase family 7 protein"
                     /protein_id="HZX18004.1"
                     /translation="MLDLTTWNLSVPSASAPSTISTARLNNGYSSQYFRRNADDSVTF
                     WVPVNGSHTADARYPRSELRETQADGSLSNWYHASADSYLNAVLTVNQVPSKNKIVIA
                     QVHSKDEPGSNNDPLLKLQYHYLRGVGRLELLLRKRPGDSGVQNILLAENIQLNQRFS
                     YELRITPSGTLGIKVTSSHGDNGSFYQQLSGYWSTQLLYFKAGAYVQDNYGPSTEGAR
                     VTFYWLNSLHR"
     gene            10442..12286
                     /locus_tag="VFF22_14240"
     CDS             10442..12286
                     /locus_tag="VFF22_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZX18005.1"
                     /translation="MPPFAPARRVDVLYWLSTWLAVAIHLGQLFEPVESLGMGVYQGL
                     LISSYALLFISPFWLLCRLCGRWLGAARIVLLIALASALQLLIYADMLLWKLYGFHLN
                     GFVWNILTTPGGIAALGSSESSERGFALIALATVAGQSMLYLLAAALVRKVRGVPVPR
                     LLWVLPLFILLAVGERTSYALSHFYGYSPLLETAQRMPFYQPLTMRGFLEKRLGLERP
                     QRMEVENVALKGQLAYPQAPLRIEPPAKPLNVVWLVAESWRADSLNPRVMPQTNAFAE
                     RAQRFDNHFSGGNGTRIGMFSQFYGLPANLWFPVLDARVGSPVIDVLQQQQYQIKLFT
                     SAKFSYPEFDKTLFVKVPAEQMQAFDQGHSWQRDRNNVDDLLQFIDQRAPTKPFMTFM
                     FFESPHANYEFPPESIIEPDYLPDFSYASMDLERDIQGIYKRYLNSVHHLDSQIARVT
                     EHLQAKGLLDSTLIVITGDHGEEFMEKGRWGHNSTFVDEQIKVPLVLWVPGQAAHREV
                     LPTSHVDLLPTLLPLLGVNNPPRDYSIGQSLFKPSAKRMLIAGDWDRLAFMDGQHKVV
                     LPFSSGSFTAMQTSHADDRPIVDASRLLPLAMPVIRSELQGIRRFLAH"
     gene            12368..12814
                     /locus_tag="VFF22_14245"
     CDS             12368..12814
                     /locus_tag="VFF22_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZX18006.1"
                     /translation="MARLKLEFPEDQFCYATHLTVRVTDINAANHLGNDSMISMISEA
                     RARFLFEFGIEETQEDGTGIIVTDLATTYRAEAHARDQLLFEVGVMDFNTYGGDITFR
                     ITRPADGALVAMAKSGFVFFNYKLGKVVQMPATFSGKFSKVNWLGK"
     gene            13613..14824
                     /locus_tag="VFF22_14250"
     CDS             13613..14824
                     /locus_tag="VFF22_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HZX18007.1"
                     /translation="MNTSFWKSGHTPTLFAAFLYFDLSFMVWYVLGPLAVQIATDLDL
                     TAQQRGLMVATPILAGAVLRLLMGFLADRLSPKTAGLVGQVIVIVALFCAWQIGISSY
                     EQALLLGLFLGFAGAAFAVALPLASQWYPPQHQGKAMGIAGAGNSGTVLAALFAPGLA
                     ALFGWQNVFGWALIPLLATLLIFALVAKNSPERPKPKALADYLKALGDRDSWWFMFFY
                     SVTFGGFIGLASALPGYFNDQYGLNPVTAGYYTAACVCAGSLMRPLGGALADRIGGIR
                     SLLMMYTLASLSIAAVGFNLESSAAALTLFVTAMLGLGAGNGAVFQLVPQRFRKEIGV
                     MTGLIGMAGGIGGFLLAAGLGTIKQQTGDYQLGLWLFASLGVLAWFGLYGVKMRWRTT
                     WGSAAVTAARV"
     gene            15063..16730
                     /locus_tag="VFF22_14255"
     CDS             15063..16730
                     /locus_tag="VFF22_14255"
                     /EC_number="2.7.11.-"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HZX18008.1"
                     /translation="MALQLTFGEASATGPRSENQDAIRVVTPVPALAASKGYLFALAD
                     GVSQCADGGLAARATLQALALDYYSTPETWAVTQSLDRVLVAHNRWLQANGGGQPLLT
                     TLSALILRGRRFTLAHVGDCRAYRWLDGTLERLSEDHVWEQPGMQHVLKRAMGLDQHL
                     VMDYLDGELREGECFVLVSDGIWATLGDAGIQRILHDEQDLPAASSALVNAAHLAGSQ
                     DNASALLLRVEALPQSDLADTLAQLQHWPLPVKLREGQLFEGWEVERLLAESRQSLVY
                     RVRDAQARRWLLKTLPPGRTDEPHAGPALLLEEWFLRRVAGRFFAEVHPLPQRQHLYY
                     VQREYAGQTLAEHLRLSGPLGLPEWLDIAPRLIKALGMLHRRNILHRDIKPENLLWGD
                     DGELRVLDFGLAYCPGLTRDEACELPGTPSYIAPEAFTGVSPSPQQDLYSAGVTLYFL
                     LTGHYPYGEIEAFQHPRFGQPTPASRYRPDLPSWVDDSLNRALSAEPKQRYETAEEWL
                     LNLEQAERKSLSSRPRPLLEREPLLVWRGVALFSLLLNLLLLIGLLR"
     gene            17028..17765
                     /gene="cobA"
                     /locus_tag="VFF22_14260"
     CDS             17028..17765
                     /gene="cobA"
                     /locus_tag="VFF22_14260"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HZX18009.1"
                     /translation="MSAKVWLVGAGPGDPELLTLKAVKALNQAEIVMIDDLVNPAVLE
                     HCRNARIMPVGKRGGCRSTPQAFIHRLMLRYARQGKCVVRLKGGDPCIFGRGSEEADW
                     LNEHGIEVEMVNGITAGLAGATNCGIPLTLRGVARGVTLVTAHTQDDSSLNWSALAQS
                     GTTLVVYMGVAKLPEIRESLLAGGMRADMPVAMIENASLPHQRECRSSLAAMQQAAHD
                     FQLKSPAILVIGEVAAQPNMQLAAISA"
     gene            complement(17980..18945)
                     /locus_tag="VFF22_14265"
     CDS             complement(17980..18945)
                     /locus_tag="VFF22_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020290163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZX18010.1"
                     /translation="MKLKNTLGVVIGSLLATSSLSVLAQGQGAVEVEGFAKKEMFDSA
                     RDFKNNGNLFGGSIGYYLTDDVELRLAYDEVHNARGEDGRNIKGSNTALDALYHFNNA
                     GDALRPYVSAGFSDQSIGQSGRGGRNGSTFANIGGGAKYYFTDNFYARAGVEAQYNID
                     QGDTEWAPSVGVGMNFGGGSKPAPAPVAAVEPTPEPVAVEEPMELVRVELDVKFDFDK
                     ASVKEESYGDIKNLADFMKQYPQTTTVVEGHTDSVGSDAYNQNLSERRASAVRDVLVN
                     QYGVDAARVNAAGYGEARPVADNATDAGRAVNRRVEAEVEAQTQQ"
     gene            complement(19045..19644)
                     /gene="sigX"
                     /locus_tag="VFF22_14270"
     CDS             complement(19045..19644)
                     /gene="sigX"
                     /locus_tag="VFF22_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019689476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigX"
                     /protein_id="HZX18011.1"
                     /translation="MNKAHSASLRYDPRELSDEELVARAHDELFHITRAYEELMRRYQ
                     RTLFNVCARYLGNDRDADDVCQEVMLKVLYGLKNFEGKSKFKTWLYSITYNECITQYR
                     KERRKRRLIDALSLDPLEEASEEKAPKPEDKAGLDRWLVHVNPIDREILVLRFVAELE
                     FQEIADIMHMGLSATKMRYKRALDRLREKFADSTETYLE"
     gene            complement(19733..>19871)
                     /locus_tag="VFF22_14275"
     CDS             complement(19733..>19871)
                     /locus_tag="VFF22_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HZX18012.1"
                     /translation="QQVQVGAVEGQIEEIGTVKTTLLTEAGELVSVANRTLLEQQVSS
                     R"
BASE COUNT         4074 a         6319 c         5608 g         3870 t
ORIGIN      
        1 tgagcaaccg gccgaaagca ccaagggttt caccctggcg cagaaaatgg ttggtcgcgc
       61 gtgcggtgta actggcgtac gtccaggcac ctactgcgag ccgaagatga ccaccgtcgg
      121 ttcccaggac accactggcc cgatgacccg cgacgagctg aaagacctgg cttgcctggg
      181 cttctccgct gacctggtaa tgcagtcgtt ctgccacact gcggcttatc cgaagccaat
      241 cgacgtcacc acccaccaca ccctgccgga tttcatccgc acccgtggcg gcgtgtccct
      301 gcgtcctggc gacggcatca tccacagctg gctgaaccgc atgctgctgc cagataccgt
      361 cggcaccggt ggcgactcgc acacccgctt cccaatcggc atctccttcc cggccggctc
      421 tggcctggtt gccttcgccg ctgccactgg cgtaatgccg ctggacatgc cggagtcggt
      481 actggtgcgc ttcaagggca aactgcaacc gggcatcacc ctgcgtgacc tggtgcatgc
      541 catcccttac gtggccatcc agaaaggcct gctgaccgtc gagaagaaag gcaagaagaa
      601 catcttctcc ggccgcatcc tcgagatcga aggtctggac gagctgaccg ttgaacaggc
      661 tttcgagctg tccgacgcct ccgccgagcg ttccgctgca ggttgcacca tcaagctgcc
      721 ggaaaaggcc atcgccgagt acctgcgttc gaacatcacc ctgctgcgct ggatgatcag
      781 cgaaggctac ggcgatgccc gcaccatgga gcgtcgcgcc caagcgatgg aagcctggct
      841 ggccgatccg aagctgatgg aagccgacaa ggacgccgag tacgccgaaa tcatcgaaat
      901 cgacctggcc gacatcaacg agccggttct ctgcgcaccg aacgacccgg acgacgcgcg
      961 tctgctgtcc tccgttcagg gcgagaagat cgacgaagtg tttatcggtt cgtgcatgac
     1021 caacatcggt cacttccgcg ctgccggtaa gttgctggaa aaagtcgaag gcaccctgcc
     1081 gactcgcctg tggctgtcgc cgccgaccaa gatggaccag catcagctca ccgaagaagg
     1141 ctactacggc atctacggca aagccggtgc gcgcatggaa atgccgggct gctcgctgtg
     1201 catgggtaac caggcacgcg ttgaggcgaa ctccaccgtg gtgtcgacct ccacccgtaa
     1261 cttccccaac cgtctgggcg atggtgccaa cgtctacctg gcctcggccg agttggctgc
     1321 tgttgccgca atcaccggca agctgccgac tgttgaggaa tacatggtct acgcgaagaa
     1381 aatcgacaca atggcagcgg acgtttaccg ctacctgagt ttcgatcaga tcgccgagtt
     1441 ccgtgaagct gctgcaaacg cccagatccc tgctgttcag gtctaagcgt tacccgcaac
     1501 aagaaagccc cgcctagtgc ggggcttttt cttttccgtc gtactagctc gcagacagat
     1561 ggcatatacc ttgctctggc ttaagccatc aggcttttcc aacggcggaa atggctgcaa
     1621 tagacaatgg tgtcgttctg atcacagact gaagtcggtg atcggcacgg cacctttttg
     1681 tgtgggcaca ggaaacgcac catgaatgag cacgacatca gccgcagcga tactccggaa
     1741 aaaccggtac tgaatatcgg ctttatggcc ctcacagacg ccgcatcggt catcgtggca
     1801 gccacccagg gcttcgccga gaagcatggg ctgaccctca acctgcagcg ccaggcctcc
     1861 tgggccactc tgcgcgacaa actactaagc ggcgagctgg acgccgccca tagcctctac
     1921 ggcttgattt atggcgtgca gctgggcctg ggcggtgcgc cagccaccga tatggcggtg
     1981 ctgatgggct tgaaccagaa cggtcagagc atcaacctgt cgcccgcctt gcaggccgct
     2041 ggcgtgagca ctggcagcgc attggcgcaa cgcgcgcacc agtctggcgc aaaactgact
     2101 ttcgcccaga ccttccccac cggcacccac gccatgtggc tgaattactg gctggccagc
     2161 cagggcattc acccactcag cgatgtcgac agcgttgtcg tgccgccacc gcagatgctc
     2221 gcccacctca aggcccggcg tatcgacggc ttctgcgtct gtgaaccctg gagcgcccag
     2281 gcagtggatg aacaaatggg ctgcaccctg accaccagcc aggcgatctg gcccgatcac
     2341 ccggagaaag ttctgggttg cacacgagag tttgccgaac agcaccccaa cagcgctcgc
     2401 gccctgatca tggcggtact ggaagccagc cggtttatcg atgaaagcga agaaaaccag
     2461 cgcagcacgg cgcaactgct cagcggcagc gagtatgtcg acgcgccgct gtcggcgatc
     2521 cagccgcgct ttctcggcca gtaccaggac ggcctcggcc atgcatggct ggacgcccac
     2581 ccgctgcgtt ttcacgcaga cggcgcggtc aaccaaccgt acctgtccga cggcatgtgg
     2641 tttatgaccc agttccgccg ctggggcctg ctgcgcgaag atccggacta cctggctatc
     2701 gcccgccagg tgcagcaact tgacctctac cgtcaggctg ccgatgcact tggcatagcc
     2761 gttccaagcg agatgcgcag cagccaactg ctcgacggcc aagtctggga cggcagtgat
     2821 ccggccggct acgcgcgcag ctttgccctg cacgcactgg ccgatgcatc catcggcgcc
     2881 caacaggaga gccgctaaca tgctgcgcat cctgctgatc aacgacaccg ccaagaaggt
     2941 cggccgcctg aaaaccgccc taaccgaggc cggtttcgag gtcatcgacg aatccgggtt
     3001 gaccatcgac ctaccggaac gggtcgaggc ggttcgccct gacgtgattc tgatcgacac
     3061 cgaatcgccg agccgcgacg tgatggaaca ggtggtgctg gtcagccgcg atcagccgcg
     3121 gccgattgtc atgtttaccg acgagcacga ccccggcgtg atgcgccagg cgattcagag
     3181 cggcgtcagc gcctatatcg tcgagggcat ccatgctcag cgcctgcagc caattctcga
     3241 tgtggccatg gcccgtttcg aaagtgacca ggctctgcgc gcacagctgc aagcgcgcga
     3301 tgcgcagttg gccgaacgca aacgcatcga actggccaaa ggcttactga tgaagatgaa
     3361 aagctgcgat gaagagcagg cctacaccct gatgcgccgt caggccatga gccgtcagca
     3421 gaagctggcg caagtagccg aacagatcat cgccatgcac gatatgctcg gcggctaacc
     3481 accgctcctg tagcccggat gcaatccggg ctacgaccta ccagcctaca gctgtactgc
     3541 cgagtttcac gacaactgct ctagacagta ccactgcgcc ccgcttatct tcgccatctg
     3601 gccagcacac tggccaacgt cgctcggacg gttccgggcg cttacgtatc cgaggaaagc
     3661 atggctgacc aagcttcgcg ccgctttgcg cgcatcgatc gactcccccc ctacgttttc
     3721 aatatcactg ccgaactgaa gatggccgcg cgtcgccgtg gcgaagacat catcgatttc
     3781 agtatgggca acccggatgg ccccacgccg ccgcatatcg tcgagaagct ggtgcaggtt
     3841 gcccagcgtg aggacaccca cggctactcc acctcgcgcg gtattccgcg cctgcgccgc
     3901 gccatctcgc gctggtacaa ggaccgctac gaggtcgaga tcgatccgga aagcgaagcc
     3961 attgtcacca tcggctccaa ggaaggcctg gcgcacctga tgctggccac cctggaccat
     4021 ggcgacacgg tacttgtacc gaaccccagc taccccatcc atatctatgg cgcggtaatc
     4081 gccggggccc aggtacgctc ggtaccactg attccaggcg tagacttctt cgcagagctg
     4141 gagcgggcga ttcgcgagtc tatccccaag ccgaagatga tgatcctcgg cttcccctcc
     4201 aaccccaccg cacaatgcgt ggagctggac ttcttcgaac gcgtggtggc cctggccaag
     4261 cagtacgacg tgctggtgat ccacgacctg gcctacgccg atatcgtcta cgacggctgg
     4321 aaagccccgt cgatcatgca ggtgccgggc gccaaggaca ttgctgtgga gttcttcacc
     4381 ctgtcgaaga gctacaacat ggccggctgg cgcatcggct ttatggtctg taacaaagag
     4441 ctggtcagcg ccctggcgcg gatcaagagc taccacgact acggcacctt caccccgctg
     4501 caggtggcgg cgattgccgc gctggaaggt gatcagcaat gcgtgcgcga tatcgccgag
     4561 caataccgcc agcgccgcaa cctgctggtc aaaggcctgc acgagttggg ctggatggtc
     4621 gaaaacccga aagcctcgat gtatgtctgg gcgaaaattc ctgagcagta cgccgcgctc
     4681 ggttcgctgg agttcgccaa gaaactgctg ctggaggcta aagtctgcgt ctcgccaggc
     4741 attggctttg gcgattatgg cgatgaccat gtgcgctttg ccctgattga aaaccaggac
     4801 cgcatccgtc aggcgattcg cgggatcaag acgatgttcc gcgccgatgg cctgttaccc
     4861 ggcaagaagg ccaaggccga cgagtaaaca acagagggca ccttagggtg ccctctttcg
     4921 tttcagccgg agtaatccag caccgtccaa accttactta agccactggt aattacgcag
     4981 ccgctctgcc cgagttgatt gttgctatgc agcgacatca ggttggtcat gccatccgcc
     5041 gcatccgagc gctcgctcag ccactgcacg cggccgcagt tggccgtttc gatcacataa
     5101 ctggcagcca cggcagagtt ctcgctcggc agcctgatca catccaccac cgtaccctgc
     5161 tcttctacgc gcttgccgtc ggataccagc accgcccagg cctggccatt ctcgatcact
     5221 aggtaggtgc cattggccct cggttgatca agctgcggtt gtgcacaacc ggccaaaaga
     5281 cccaacagca caattgtcat cagcatctgt tgcattgtta gtgactcctc tgcagtgcct
     5341 gtggccattg gctttgcggg cagatggcgc gagtgatcga gtaggtgccg gttaggacct
     5401 tgggcttact attatcgaat actgtttatt tgtacagtat ttttcgatta cccttggtca
     5461 tgccatccag ccaaccatgc cgcccgtcag atcgggccca tacaaggcgt taacaggagt
     5521 accccagatg ctcgacgtac tgcagctgaa acacagcgta aaccgcatgc ccctggaaaa
     5581 agtccgggcc accgtcaatg aattgcagct tgaaggcatc gtcaccgagg gcaaaacgcc
     5641 gttttcccgg gtgcacttca acacctgctt tgccgagatc gaagccttgc tgcagcgcgc
     5701 cggctatcac cgtcaactgg atgtggtcgg ctaccagggg ctggcctatg cgatgttcga
     5761 cccctcgcgc tgggaggcgg tagaggtgct gcgctggctc aaggagtttg tcgaggaagc
     5821 acagggcaaa acgctgtcgc gctaaggcgg ctagcgctta acctctgggg tgacctagca
     5881 ccaaggcgaa gcaaacgcgc tcggcagcgg cttggctacg cacagcattg gctcattgaa
     5941 tctgataaat acccttatcg gccagtgcct ggacaacctg ccaatcaggc caaaaaatct
     6001 attatcgaca ttaaaataaa aagtaaatct ttgatttata aagctatata agccgatcct
     6061 cagactatat cgagctgccc tcagtccatg tctaaacgcc gttacggcaa tacctggaga
     6121 aagctggcaa ggcctttgct tattcaccct caaggcaaca acacacgtct tcagatcgcc
     6181 aacggcggtg atcaggacga attgacaaag acgtcaacga cactcggcca actactgggc
     6241 gagctgtccg cgacgtcttt tttcgtttct gggacttgag gaaccactat gcgtaactcc
     6301 accgcacgcc gttcgctact gaagaaaacc ctggccggac tcggctgcgc cgccgtcatg
     6361 ctgctgtcgc cactgagcgt gcagatcacc ctcgccgccg aggcggaaaa ggacgaactg
     6421 aagttcggct ttatcaaact gaccgatatg gccccgctgg ccatcgctta tgagaaaggc
     6481 ttcttcgagg atgaagggct gtacgtcacc ctggaagccc aggccaactg gaaggtgctg
     6541 ctggatcggg tgatcgacgg cgaactggac ggcgcgcaca tgctctccgg acaaccaatc
     6601 ggcgccacca tcggtttcgg caccaaggcc gatgtggtta ccgctttcac catggacctc
     6661 aacggcaacg ccatcaccgt ctccaatgca gtctgggagg agatgaagaa acatgtgccc
     6721 atggaaggcg gtaagccggt acacccgatc aaggccgacg ccctgaagcc ggtgatcgac
     6781 aagttcaacg ccggcggcaa gtcgttcaac ctgggcatgg tgttcccggt gtccacgcat
     6841 aactatgagc tgcgctactg gctcgctgcc ggcggcatcc acccgggtta ttacgccccg
     6901 cacaagggcg acaccagcgg ccagattgac gccaaggccc tgctctcggt caccccgcca
     6961 ccgcagatgc ccgccaccat ggaagccggc accatccacg gttacagcgt cggcgagccg
     7021 tggaaccagg cggcggtgtt caagggcatc ggcgtaccgg tagtggccga ctacgcgatc
     7081 cgcaagaaca tgtcggagaa ggtcttcggc gtatccaaga gctgggccga agccaacccg
     7141 gaaacccaca agcgcgtagt caaggcgctg attcgcgccg gccagtggct ggacgcggac
     7201 aacaacgcca accgtcagga agcggtggag atcctttccc gccccgaata tgtgggggcc
     7261 gatgccaagg tgatcgccaa ctcaatgacc ggcaccttcg agttcgagaa aggtgacaag
     7321 cgcgaagtac cggacttcaa cgtgttcttc cgctaccacg ccacctaccc ctactactcc
     7381 gatgccgtgt ggtacctgac gcagatgcgc cgctggggcc agattgccga aaccaaaccg
     7441 gacagctggt acctggagac ggccaagaag gtctacctgc cggctatcta ccgcgaagcg
     7501 gccgccgaat tgatcgcaga aggcaagttc gcagccagcg atttcccgcc agccgatgac
     7561 gacggtttcc gcgcgccatt gaccgacacc atcgacgctg tgccttacga cggtcgcaag
     7621 cccaatgcct acctcgagca gttcgagatc ggtttgaagg gcgacacgca gctctaacag
     7681 actgctgcat aggcactccc ggagccggta cgccggctcc cttctaacaa agaggacagg
     7741 acgatgagca acccgagcgt cgccaccccg ataaccgcaa cacttaagtc ggcgcgcagc
     7801 agtgcgctcc tcaagcgctg ggcccccgcg ctgatcctgc ccatgctggg catcatcggt
     7861 tttctgctgt gctggcagct gctggccaac aatatccaga ccagcctcgg ccagttcccc
     7921 ggcccggtgc aggtctccga acagttttcc ggcctggtaa gcgcccattt caaggagcgt
     7981 gaaaaagccc gggtcttcta tgagcgccag gacacccgca acgccgcctt gctggcgaaa
     8041 aaccccgacg ccgagatcaa ggtgcgccct tataccggca agccgacctt tattcagcag
     8101 atattcacca gcctctacac cgtcatggcc ggctttgtca tcgcttcgct gatcgccatt
     8161 ccgctgggta tcgtctgcgg cctgagcaag agtatctaca ccgccatcaa ccccctgatc
     8221 caggtgttca agccggtctc cccgctggcc tggttgccgc tggtgaccat gctggtcagc
     8281 gctctgtatg tcactgacga tccgctgttc gccaagtcct tcctcacctc ggccattacc
     8341 gtggcgctgt gctgcctgtg gccgacggtg atcaacacca cggtgggcgt ggccagcatc
     8401 gagcaggacc tgaacaacgt cagcaaggtg ctcagtctgt cgccgctggc gcatgtgcgg
     8461 cgcatcgtcc tgcccgccgc cgtgccgatg attttcaccg gcctgcgtat gtcgctcggc
     8521 accggctgga tggtgctgat cgccgcagag atgctggcgc agaacccagg cctgggcaaa
     8581 ttcatctggg acgagtttca gaacggcagc tccaactccc tcgggcggat catggtggcg
     8641 gtgattgtca tcggcctgat cggcttcgcc ctcgaccgcc ttatgctcct gctgcaacgt
     8701 cagttcagct gggacaagaa tgccgtactg cgctaatcag gagacttccc atgagccatt
     8761 cacacctcga actgaccggc gtcggcatcg acttccccac cgacaagggc ctgtaccgtg
     8821 ccctgcaaaa cgtcaacctg cgcatagaca agggcgaatt cgtctccctg atcggccact
     8881 ccggctgcgg taaatccacc gtactgaaca tagtcgccgg cctgtataag gccaccaccg
     8941 gcggggtcat cctcgacggc cgcgaggtcg acgagcccgg ccccgagcgc gcggtggtat
     9001 tccagaacca cagtctgctg ccttggctaa gctgctacca gaacgtcgag ctggcggtac
     9061 gtcaggtgtt ccaaggcaag aaatcgcgca gcgaaatgcg cgactggatc gaacacaatc
     9121 tgcagctggt gcacatgatg catgccaagg acaagcgccc cagcgagatc tccggcggca
     9181 tgaaacagcg catcggcatt gcccgtgccc tggccatgca gcccaaggtg ttgctgatgg
     9241 acgaaccctt cggcgcactg gatgccctga cccgcgcgca tctacaggac tcgctgatgg
     9301 agatccatgc agacctcggc aataccgtga tcatgatcac ccacgatgtc gacgaggccg
     9361 tgctgttgtc cgaccgcatc gtgatgatga gcaacggccc ggcagccagt gtcggcgaga
     9421 ttcttcaggt gcaactgcag cgcccacgtg atcgcattgc cttggcccat gacgcgcgct
     9481 accacgaata ccgagccgcc gtgttggagt tcctctatca gaaacagcaa cgtccggctg
     9541 cgtaagacac ggatttgccc gtcgcgctca ccctggctgg gcgcgacggg caaagatgcg
     9601 cagccgccga tcaggccaat tgaacttcat ccgccgatcc tcaacgaaca ggagcccttc
     9661 ttcttccgct aaggatctcc tgtgctcgac ctcaccacct ggaatctctc cgtgcccagc
     9721 gcgtccgcgc cgagcaccat cagcaccgcc cgcctgaaca acggctacag cagccagtat
     9781 ttccgccgca acgccgacga cagtgtgacc ttctgggtac cggtcaacgg ctcacacacc
     9841 gccgacgccc gctacccacg cagcgagctg cgcgagaccc aggccgatgg cagcctaagc
     9901 aactggtacc acgccagcgc cgacagctac ctaaacgcgg tgttgacggt caatcaggtg
     9961 ccgagcaaga acaagatcgt gatcgcccag gtacacagca aggacgagcc cggcagcaac
    10021 aacgaccccc tgctgaaact gcagtatcac tacctgcgtg gcgtcggccg cctcgagctg
    10081 ttgctgcgca agcgcccggg cgacagcggc gtacagaaca ttctgctggc ggagaatatc
    10141 cagctgaacc agcgtttcag ctacgagctg cgcatcactc caagtggcac gctcggcatc
    10201 aaagtcacca gcagccatgg tgataatggc tcgttctacc agcaactgag cggatactgg
    10261 agcacgcaac tgctctactt caaagccggc gcctacgttc aggataacta cggccccagc
    10321 actgaaggtg ctcgggtgac cttctattgg cttaacagcc tgcaccgcta gttaagccct
    10381 gcttaagcaa gcagccttat gctgcaaagc ttcatgccac acctctaata aggaactgtc
    10441 tatgcccccc tttgcgcccg cgcgccgcgt tgatgtgctg tattggctgt ctacctggct
    10501 tgccgtagcc attcacctcg gccaactgtt cgaaccggtc gaatcactgg gcatgggggt
    10561 gtatcagggg ctgttgatca gcagctatgc cttgctgttt atcagcccgt tctggctgct
    10621 gtgccggctg tgcggacgct ggctgggagc agcgcgaatt gtcctgctga ttgccttggc
    10681 cagtgcgctg caactgctga tctacgcaga catgctgctg tggaagctct atggctttca
    10741 cctcaacggt tttgtctgga acattctgac tactcccggc ggtattgccg cactgggctc
    10801 atcggaatcc agcgagcgcg gttttgcctt gattgccctc gccacagtgg ccggccaaag
    10861 catgctctat ctgctggctg cagccctggt gcgcaaggtt cgcggcgtgc cagtaccgcg
    10921 cctgctgtgg gtgctgccgc tgtttatcct gctggccgtg ggtgagcgaa ccagctatgc
    10981 gctgagccat ttctatggct acagcccact gctggaaacc gctcagcgca tgccgtttta
    11041 tcaaccactg accatgcgcg gcttcctcga gaaacgcctg ggcctggagc gcccgcagcg
    11101 tatggaagtg gaaaatgtcg ccctgaaagg ccagctggcc tacccacaag cccccttgcg
    11161 tattgaaccg ccggccaagc cattaaacgt ggtctggctg gtggccgagt cctggcgtgc
    11221 ggacagcctc aacccgcggg taatgccgca gaccaatgct ttcgccgagc gcgcgcagcg
    11281 tttcgacaat cacttttccg gcggtaacgg cacacgcatc ggcatgttca gccagttcta
    11341 cgggctgccg gccaacctct ggttcccggt gctggatgcg cgggtgggca gcccggtgat
    11401 cgatgtgctg caacagcagc agtaccagat aaagctgttt accagcgcca aattcagcta
    11461 cccggagttc gacaaaaccc tgttcgtcaa agtccccgca gagcagatgc aggcctttga
    11521 ccaaggccac agctggcagc gtgaccgcaa taatgtcgat gacctgctgc agttcattga
    11581 ccagcgcgcc cccaccaagc cctttatgac ctttatgttt ttcgagtcgc cccacgccaa
    11641 ctacgaattc ccacccgagt cgatcatcga accggattac ctgccggact tcagctacgc
    11701 cagcatggat ctggagcgcg acatccaagg catctacaag cgctacctca actcggtgca
    11761 ccacctcgat agccagatag cgcgcgtcac tgagcatctg caagccaagg gactgctcga
    11821 cagcacgctg atcgtgatca ccggagacca cggcgaagaa tttatggaaa aaggccgctg
    11881 ggggcacaac tcgacctttg tcgacgagca gatcaaggtt ccgctggtgc tttgggtacc
    11941 tggccaggcg gcacatcgcg aagtgctgcc gaccagccat gtcgacctgc tgccaaccct
    12001 gctgccgctg cttggggtga ataacccacc gcgcgactac agcatcggtc aaagcctgtt
    12061 caaacccagc gccaaacgca tgctgattgc cggcgactgg gaccgcctgg cctttatgga
    12121 cggccagcac aaggtggtac tgccgttttc cagcggcagt ttcaccgcca tgcaaaccag
    12181 ccacgccgac gaccgcccga ttgtcgatgc ctcgcggcta ttgccactgg ctatgccggt
    12241 gatacgcagc gaactgcagg gcattcgccg ctttctcgca cattaaatag atcgggcgta
    12301 tccagtcatg gatggcgcca gccgctagtg ggtaaactgg cggtctttct cgactgcgat
    12361 cttgaccatg gcccgcctga aacttgagtt ccctgaagac cagttctgct acgccaccca
    12421 cctgaccgtg cgagtaaccg atatcaatgc cgccaaccac ctgggcaatg attcgatgat
    12481 ctcgatgatc tccgaggcgc gggcgcgttt tctgtttgag ttcggcattg aggaaaccca
    12541 ggaagacggc accggcatta tcgtcacgga tctagccacc acctaccgcg ccgaggccca
    12601 cgcccgcgat cagttgctgt tcgaggttgg tgtgatggac ttcaacacct atggcggcga
    12661 catcaccttc cgcattaccc gccccgccga tggcgcactg gtggccatgg ccaagtccgg
    12721 ttttgtgttc ttcaactaca aactgggcaa ggtggtgcag atgccggcga ccttcagcgg
    12781 caaattcagc aaggtcaact ggctgggcaa gtaatctggg cttagcggca taaagcgcgc
    12841 gattgccggc cggatcacgc ttgatcgcgg caatgcgacc caattgcacc taaattgcgc
    12901 aaccaccgtg acagctggca catcctctgc tacgccttag gcataacaac agcaggggaa
    12961 ttgctatgcc gaccacgcgc agccatgtcc gccaagccgg gctcttgagc aacctactcg
    13021 ctctggccct cctcaccctt cccttaccga cccaggctgc aagcacagcc gccgacgcca
    13081 catccgatga atcacgctgc agcgcgctaa cgcgtatcag ctgaaacact cgccccaagg
    13141 ccaatcgtgg cctgtcctct accgcctggt ttgtcagaac ctggaacagc ttctgcgccc
    13201 cattcaaggg caagccattt gataacgcct ccttttgcag cacgatcatg acgatccaac
    13261 agctgaaagc ccgtgagtcc acgggcttag cccggttggc gcagctcttg ctatagccct
    13321 gttacaggta gccaatggcg gctgctcaac aaacgacaaa gacgtcgcat aaaccacgcc
    13381 ccacggcgtt cggcttatgc ggcgttttgc gttttacgcc ccagcgtttg gggctggcac
    13441 ctgccctgcg atccaggact cgcgctagta actctctcaa cccagctgtg gcttcggcct
    13501 cagggtggcg taccacaagt acaccacctc cccgatgggt tgcggccgcc gtgtccacga
    13561 ccatggcgta ccgcacccca ccgggaccct tttttgctga atgaggtttt cgatgaatac
    13621 aagtttctgg aaatccggcc acaccccaac cttgttcgcc gcgtttctgt atttcgacct
    13681 gagctttatg gtctggtacg tgctcggccc actggctgta cagatcgcca ccgatctcga
    13741 cctgaccgcc caacagcgcg gcctgatggt cgccacgccg attctcgcgg gcgccgtgct
    13801 gcgcctgctg atgggtttcc tggccgatcg cctatcaccc aaaaccgccg gcctagttgg
    13861 tcaggtgatc gtcatcgtgg cgttgttctg cgcctggcag atcggcatca gcagctatga
    13921 acaggcctta ctgctcggtt tgttcctcgg ttttgccggt gccgcgttcg ccgtggccct
    13981 gcccctggcc tcgcaatggt atccgccgca gcaccagggc aaggccatgg gtattgccgg
    14041 tgcgggcaac tctggcactg tgctggctgc actgtttgcc ccgggtttgg cggcgctttt
    14101 cggttggcag aacgtattcg gctgggcact gatcccgcta ctggcaaccc tgctgatctt
    14161 cgccctagtg gccaagaact ccccggagcg gcccaagcca aaagccctgg ccgattacct
    14221 gaaagccctg ggtgatcggg acagctggtg gtttatgttt ttctacagcg tgaccttcgg
    14281 cggctttatc ggcctggcca gcgccctgcc cggttatttc aacgaccagt acggcctgaa
    14341 tccggtgacc gccggctact acaccgccgc ctgcgtctgc gccggtagcc tgatgcgacc
    14401 attgggcggc gcactggccg accgtatcgg tggcattcgc tcgctgctga tgatgtacac
    14461 cctggcttcg ctgagcattg cagcggtggg cttcaacttg gaaagctcgg ccgccgcact
    14521 gaccctattc gtcaccgcca tgctcggcct tggtgccggt aacggcgcgg tcttccagct
    14581 ggtgccacag cgctttcgca aggaaatcgg agtaatgacc gggctgatcg gtatggccgg
    14641 cggcatcggt ggcttcctcc tggcagcagg cctgggcacc atcaagcagc agaccggtga
    14701 ttaccaactc ggcttgtggc tgttcgccag cctcggtgta ctggcctggt ttggtctgta
    14761 tggggtcaag atgcgctggc gcaccacctg gggcagcgcc gcagtcaccg ccgctcgggt
    14821 gtaatgacgc aaacctaggg tggatgacgc ttttcatcca ccctggagca acggtggatc
    14881 gctaaagcgc gatccaccca cgccaaacca ttggagcatt gaggcaaagg cgggactgac
    14941 tggcagcgct tattgcactg gtcaggaatg gcgggagaag tcggcagcgg ctctcctttg
    15001 ctgcctcacc agcctcgcct ttgcctcagc gcttcttcag caacacaacc aggaacttca
    15061 gcatggcctt gcaactgacc ttcggcgaag ccagtgctac cggcccgcgt agcgagaatc
    15121 aggacgccat tcgcgtggtc accccggtgc cagcgctggc ggccagcaag ggttatctgt
    15181 tcgccctcgc cgatggtgtc agccaatgcg ccgacggcgg cctggcggcg cgcgccaccc
    15241 tgcaggccct cgccctggat tactactcca ccccggaaac ctgggcggtc acccaatcgc
    15301 tggaccgcgt actggtcgcc cacaaccgct ggctgcaagc caatggcggc ggccaaccgc
    15361 tgctcactac cttgagcgca ctgatcctac gcggtcggcg ttttaccctg gcccatgtcg
    15421 gtgattgccg agcctaccgc tggttggacg gcacgctcga gcgtctcagc gaagaccatg
    15481 tgtgggaaca accaggcatg cagcatgtgc tcaaacgcgc catgggcctg gatcagcacc
    15541 tggtgatgga ttacctggat ggcgagctga gagaaggcga atgttttgta ctggtcagcg
    15601 acggtatctg ggccaccctg ggcgacgcgg gcattcagcg catcctccac gatgaacagg
    15661 atctgccagc agccagcagc gcactggtca atgccgcgca cttggccggc agccaggaca
    15721 acgccagcgc cctgctgctg cgcgtcgagg cattgccgca aagcgatttg gccgacaccc
    15781 tggcgcaact gcagcactgg ccgctgccgg tcaaactgcg tgaaggtcag ctcttcgaag
    15841 gctgggaggt cgaacgacta ctggccgaat cgcgccaatc gttggtctac cgagtgcgcg
    15901 atgcccaggc gcggcgctgg ctgctaaaaa ccctgccgcc tgggcgtacc gatgagccgc
    15961 atgccgggcc tgcgctgttg ctcgaagaat ggtttctacg tcgagtggcc gggcgcttct
    16021 tcgccgaagt gcatccgctg ccgcaacgtc agcacctgta ttacgtacaa cgcgaatacg
    16081 ccgggcaaac cctggccgaa cacctgcgcc ttagcggccc gctgggcctg ccggagtggc
    16141 tggacatcgc tccacggctg atcaaggccc tcggtatgct gcaccggcgc aacatcctgc
    16201 accgtgatat caagccggaa aacctgttat ggggtgatga cggcgaactg cgcgtgctgg
    16261 atttcggcct ggcctactgc cccggcctga cccgtgatga agcctgcgag ctgcccggca
    16321 cccccagcta tatcgccccg gaagccttta ccggcgtatc ccccagcccg cagcaggatc
    16381 tctacagcgc aggcgttacc ttgtatttcc tgctcaccgg gcactacccc tatggcgaaa
    16441 ttgaagcttt tcagcatccg cgctttggcc aaccgacccc agccagccgc tatcgtcccg
    16501 acttacccag ctgggtcgac gacagtctca accgcgccct cagtgccgag ccaaaacagc
    16561 gctatgaaac tgccgaagaa tggctgctca acctggagca agcggaacgt aaatccctta
    16621 gcagccgacc acgccccctg ctggaacgtg aaccgctgct ggtctggcgc ggcgtggcat
    16681 tgttttccct gctgctgaac ctgctcctgc tgatcgggct gctgcgctaa aggctacagc
    16741 taacgcacca atactgcgca agtcgccttt aaacagtgca gaaaggctac gcggatcggc
    16801 tgtaacatgc tgataaccct tgagaatcgc cactgcgcaa gttggcacag gctctgcatt
    16861 ggtgactaca caatttaata aagcgtggcc tcaacgaaga aggctgcact tcccgataga
    16921 acgggacttg gacaaaggcg tcctcgctag gtgactagcg ggacgccttt tttgttggca
    16981 gccatttatg gccgctaccc ggatacccaa ggcttgagga gatcaacatg agcgcaaaag
    17041 tctggctggt aggcgcaggc cccggtgatc cggagttgct gaccctcaag gcggtcaaag
    17101 ctctgaacca ggccgagatc gtaatgatcg atgatctggt caaccccgcc gtactggaac
    17161 actgccgcaa cgcgcggatc atgccggtag gcaagcgcgg cggctgtcgc tccaccccgc
    17221 aggcgtttat tcatcgtctg atgctgcgct atgcgcgcca gggcaaatgc gtggtgcgtc
    17281 tgaagggcgg cgacccgtgc atcttcggcc gtggcagcga agaagctgac tggctgaacg
    17341 agcacggtat cgaggtggaa atggtcaacg gcatcaccgc cggcctggcc ggggcgacca
    17401 attgcggcat ccccctgacc ttgcgcggcg tagcccgtgg ggtcaccctg gttaccgcgc
    17461 atacccagga cgacagcagc ctgaattggt cagccctggc tcaaagcggc accactttgg
    17521 tggtgtatat gggcgtggcc aagctgccgg aaattcgcga aagcctgctg gcaggcggca
    17581 tgcgcgccga catgccggtg gccatgatcg aaaacgcctc actgccacac cagcgcgagt
    17641 gccgcagcag cctcgcagcc atgcaacaag ccgcccacga cttccaattg aaaagcccgg
    17701 ccattctggt gattggcgag gtcgccgctc aacccaacat gcagctcgct gctatcagcg
    17761 cctaaccgct tcgcgctccg cgaagttccc cttgcccgcc accagcgggc tttttttgcg
    17821 cgggtttttc tttatggcca tcaggagtgg cgcgccataa gaatgaccgg gagtcattct
    17881 taacgttgca caacaaaagc ccgaagagtg gccggcctgg atggcaggcc ataaagaaaa
    17941 acccggccga agccgggttt tctatgcagc cagatccgat tactgctggg tctgagcttc
    18001 tacttcagct tctacgcggc ggttaacagc gcggccagcg tcggtagcgt tgtcagcaac
    18061 cggacgagct tcaccgtagc cagcagcgtt aacacgagca gcgtctacgc cgtactggtt
    18121 gaccagcacg tcacgaacag cgcttgcacg acgctcggag aggttctggt tgtaggcatc
    18181 gctgccgacg gagtcagtat gaccttcaac cacggtggtg gtctgcgggt actgcttcat
    18241 gaagtcagcc aggttcttga tgtcgccgta gctttcttct ttaacgctgg ccttgtcgaa
    18301 gtcgaactta acgtccagct caacgcgaac cagttccatc ggctcttcaa cagccactgg
    18361 ctctggagtc ggctcaactg cagcaaccgg agccggagca ggcttgctac cgccaccgaa
    18421 gttcatgccg acaccgacgc ttggagccca ctcggtgtcg ccttggtcga tgttgtactg
    18481 agcttcaacg cctgcacggg cgtagaagtt gtcagtgaag tagtacttgg caccgccgcc
    18541 aatgttggcg aaggtagagc cgttacgacc accgcgaccg gattgaccga tgctctgatc
    18601 cgagaaaccg gcagaaacgt aaggacgcag cgcgtcgcca gcgttgttga agtggtacag
    18661 agcatccaga gcggtgttag agcccttgat gttgcggcca tcttcgccac gagcgttgtg
    18721 cacttcgtcg taggccagac gcagttcaac atcatcagtc aggtagtagc cgatgctgcc
    18781 gccgaacagg ttgccgttgt tcttgaagtc acgagcgctg tcgaacattt ccttcttggc
    18841 aaagccctcc acttcgacag cgccttgacc ctgagccaga acgctgaggg aggatgtggc
    18901 cagcagcgag ccaatgacaa cgcctaaagt gtttttcagt ttcattacta atgttcccta
    18961 tcttggtggt cagtaagttg tctgacagac acaagacaac tcagccggct attctaacag
    19021 aattaaccgt aagctatgac ggctttattc aagataagtt tcagtgctat cagcaaattt
    19081 ctcacgcaac cgatcaagtg cgcgcttgta gcgcattttc gtggcactca ggcccatatg
    19141 cataatgtcg gcaatctcct gaaactccag ctctgcgaca aaacgtagca ccagaatttc
    19201 ccgatctatc ggattgacgt gcaccagcca acgatcaagg ccggctttat cttcaggctt
    19261 tggcgccttt tcctcggacg cctcttcgag cgggtccaag ctcaaagcat caataaggcg
    19321 acgtttacgc cgctctttgc ggtattgggt aatacattcg ttataggtaa tgctgtacag
    19381 ccaagttttg aactttgatt tgccctcaaa gttcttcagg ccatataaga ccttgagcat
    19441 gacctcctga cagacatcgt cagcatcacg gtcgttgcct aaataacgcg cgcacacatt
    19501 gaacaaagtg cgctggtaac gacgcatcag ctcttcatag gcacgggtaa tgtggaataa
    19561 ctcgtcatgg gcacgcgcca ccaactcttc gtccgagagt tcgcgggggt cgtaacgcag
    19621 agacgcagag tgggctttat tcaaaacgag tcgggccgac agtcaggaca taagccagcg
    19681 cagcctgcaa atcaggctgc ggattggccg catacaataa cagggattgg ccttagcggc
    19741 tgcttacctg ctgctcgagc aaggtacggt tggccactga aaccagctcg ccggcttccg
    19801 tcagcaacgt ggttttgacc gtgccaattt cctcgatctg cccttcgacc gcaccgacct
    19861 gcacttgttg c
//
LOCUS       DASRUM010000213         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1294141, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000213 DASRUM010000000
VERSION     DASRUM010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294141"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..844
                     /gene="suhB"
                     /locus_tag="VFF22_14280"
     CDS             29..844
                     /gene="suhB"
                     /locus_tag="VFF22_14280"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-phosphate phosphatase"
                     /protein_id="HZX18013.1"
                     /translation="MQPMLNIALRAARSAGEMIFRSTERLDVISVDEKEAKDYVTEID
                     RSAELLIIQALRKAFPNHGILGEESGLSEGSGDGADYLWIIDPLDGTTNFIRGIPHYA
                     VSIACKYRGRLEHAVIIDPVRQEEFTASRGRGAALNGRRLRVGNRKSLEGALLGTGFP
                     FRDSQMENLENYIGMFRSLVGQTAGIRRAGAASLDLAYVAAGRFDAFWEFGLSEWDMA
                     AGALLIQEAGGLVSDFSGSHEFLEKGQIVAGNTKCFKAVLTAIQPHLSASLKR"
     gene            complement(899..1438)
                     /locus_tag="VFF22_14285"
     CDS             complement(899..1438)
                     /locus_tag="VFF22_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HZX18014.1"
                     /translation="MNKSMLVGAVLGAVGVTAGGAVATYNIVSAPEYAEVLAVKPINE
                     TIKTPREVCKDVAVTRQAPVKDQHQIAGTAIGAVVGGLLGNQVGGGNGKKLATVAGAV
                     GGGYAGNKAQEHLQNRDTYTTTETRCSTVTDTSEKLVGYDVKYELDGKVGQVRMASDP
                     GRQIPVKDGQLVLADESAN"
     gene            complement(1564..2469)
                     /gene="secF"
                     /locus_tag="VFF22_14290"
     CDS             complement(1564..2469)
                     /gene="secF"
                     /locus_tag="VFF22_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019823695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HZX18015.1"
                     /translation="MNRTINFMGVRNIAFAFTLLLTLLALVSLFTKGLNLGLDFTGGT
                     QIELSYEQPADLGNIREQLAGAGYADAVVQSFGATTDVVVRMQGNDPELGNKVAAALR
                     QAGEQLEVKKVEFVGPQVGEELRDQGGLGLLLALGGVMLYLAFRFQWKFGVGAIVSLI
                     HDVIVTLGILSFFQITFDLTVMAALLAIIGYSLNDTIVVFDRVRENFRVLRKATLIDN
                     INISTTQTLLRTMATSISTLLAIAALLLFAGDNLFAFSVALFVGVSAGTYSSIYIANV
                     VLVWLNLTVDDLIPPVVEEEVDERP"
BASE COUNT          555 a          828 c          647 g          481 t
ORIGIN      
        1 ctttctttaa cgacccaggt gaacatccat gcagcccatg ctgaatatcg cgctgcgcgc
       61 agcccgtagc gccggcgaaa tgatcttccg ctcgaccgaa cgcttggacg tcatctcggt
      121 cgacgagaaa gaagccaagg attatgtgac tgaaattgat cgctctgccg agctgctgat
      181 cattcaggcc ctgcgcaaag ctttcccgaa ccacggcatc ctcggcgagg aaagcggcct
      241 cagcgaaggc agtggcgacg gcgccgatta cctgtggatc atcgacccgc tggatggcac
      301 caccaacttt atccgcggca tcccgcatta cgccgtcagc atcgcctgca aataccgtgg
      361 ccgccttgag cacgccgtga tcatcgaccc ggttcgccag gaagaattca ccgccagccg
      421 cggccgtggc gctgccctca atggtcgccg cctgcgcgtt ggtaatcgca agagcctgga
      481 aggcgccctg ctcggcaccg gcttcccgtt ccgcgacagc cagatggaaa acctggaaaa
      541 ctacatcggc atgttccgca gcctggtcgg ccagaccgcc ggcatccgcc gcgccggcgc
      601 agcaagcctt gacctggcct acgtggccgc cggtcgcttc gatgccttct gggaattcgg
      661 cctgtccgag tgggacatgg cagccggcgc actgctgatt caggaagcgg gcggcctggt
      721 cagcgacttc agcggcagcc atgaattcct tgaaaaagga cagatcgtgg ccggcaacac
      781 caagtgcttc aaagctgtgc tgacggctat tcagccgcac ctgtcggcct cgctgaaacg
      841 ctaatcgcga gccacaaaaa aagcgccccg aggggcgctt ttttattaca tcgcagtttt
      901 agttagccga ctcgtccgcc aacaccaact gcccatcctt gaccggaatc tgccgacccg
      961 gatcactggc catacgcacc tggcccacct tgccatccag ctcgtatttg acgtcataac
     1021 ccaccagctt ctcactggtg tcagtcactg tgctgcaacg ggtttcggtg gtggtgtagg
     1081 tatcacggtt ctgcagatgc tcttgcgcct tgttgccggc ataaccgccg ccaaccgcac
     1141 cggcgactgt cgccagcttc ttgccgttac cgccaccgac ctgattaccc agcaaaccgc
     1201 caaccaccgc cccaatcgcc gtaccggcta tctgatgctg atccttgacc ggcgcctgac
     1261 gggttaccgc cacatccttg cagacttcac gcggggtctt gatggtttca ttgatcggtt
     1321 tcacagcaag tacttcggca tactccggcg cactgacgat gttgtaagta gccaccgccc
     1381 cgcccgcagt aacacctact gcacccaata ccgcgcctac caacatcgac ttattcatat
     1441 gtgcctcctc aaaccactgc ttagcgctca acgcaccgta ctccctgcct tggagtacaa
     1501 aaaagccggg caagttcaat acctgcccgg ctcactcaat catttgcgat aaagcgcaca
     1561 gaattaaggg cgttcatcga cctcttcttc gaccaccggc gggataaggt catcgaccgt
     1621 caggttgagc cacaccagca ccacgttggc gatatagatc gacgaataag tacccgcgct
     1681 cacaccgaca aacagtgcca ccgagaaggc gaacagattg tcgccggcaa acagcagcag
     1741 tgcagcaatt gccagcaagg tggagatcga tgtcgccata gtgcgcagca gggtctgcgt
     1801 ggtggagatg ttgatgttgt cgatcaacgt cgccttgcgc agcacgcgga agttctcacg
     1861 cacccggtcg aataccacaa tggtgtcgtt caacgagtag ccgatgatcg ccagcaaagc
     1921 cgccatcaca gtcagatcga aggtgatctg gaagaacgac aggatgccca gcgtgacaat
     1981 cacgtcgtgg atcagcgaga caattgcccc tacgccgaat ttccactgga agcggaaggc
     2041 caagtagagc atcaccccgc ccagcgccag cagcagacca agcccaccct gatcgcgcag
     2101 ctcttcaccc acctgcgggc cgacgaactc gacctttttc acctcaagct gctcgcctgc
     2161 ctgacgcagc gcagcagcca ctttattacc caactcggga tcattgccct gcatgcgcac
     2221 caccacatcg gtggtggcgc caaagctctg caccactgca tcggcatagc cggcaccggc
     2281 cagttgctca cggatgttac cgaggtctgc cggctgctcg tagctcagct cgatttgcgt
     2341 accaccggta aaatccaggc ccaggttcaa ccctttggta aacaggctaa ccagtgccag
     2401 caacgtcagc agcagggtga aggcgaacgc aatattgcgt acccccatga agttgatcgt
     2461 acgattcatc acagcccctt aaatccacaa cttcttgaag tcacgcccgc c
//
LOCUS       DASRUM010000214        31896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_627352, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000214 DASRUM010000000
VERSION     DASRUM010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 31896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31896
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627352"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1204
                     /locus_tag="VFF22_14295"
     CDS             167..1204
                     /locus_tag="VFF22_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HZX18016.1"
                     /translation="MDYRQLGRTDIKVSSLCLGTMTWGEQNSSSEGFAQIERAKAYGV
                     NFIDTAEMYPVPPRAETYSKTEQIIGDYFKQRGDRADWVLASKIAGPGNGIGYIRDGQ
                     LKHNRAHITAALDASLKRLQTDWIDLYQLHWPERPTNYFGQLGYRHQDSDFTPLEETL
                     EALDEQVKAGKIRHIGLSNETPWGTMKFLQLAEQRGWPRSVSVQNPYNLLNRSFEVGL
                     AEIALREQCGLLAYSPLAFGMLSGKYENGARPAGARISLFSRFARYTNPESESACSRY
                     VALAREHGLDPAQMALAFVTAQPFVTSNIIGATSLEQLNSNLASSEITLSAEVKAGIE
                     AIHQAQPNPAP"
     gene            complement(1201..1914)
                     /gene="nfi"
                     /locus_tag="VFF22_14300"
     CDS             complement(1201..1914)
                     /gene="nfi"
                     /locus_tag="VFF22_14300"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242942.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HZX18017.1"
                     /translation="MASLSELVAAPFSGWDGSAAAAREMQKHLATQVRLEDDYPPLRV
                     IAGVDVGFEEGGTITRAAAVLLDADSLEPLAESVARIPTSMPYVPGLLSFRELPALLS
                     ALAGLPREPDLIFVDGHGIAHPRGLGIAAHLGVVSGLPTIGVAKKILTGSHAELPQQR
                     GARAPLLDRRGSAIGWVLRSKDAVRPLIVSPGNRVSLDSAVALTMACVRRHRLPEPTR
                     LADRLASRRDARRDQASLF"
     gene            complement(2012..3148)
                     /locus_tag="VFF22_14305"
     CDS             complement(2012..3148)
                     /locus_tag="VFF22_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZX18018.1"
                     /translation="MIPRTIFSSEHEQFRDSVRKFLEQEAVPFHQQWEKDGHIDRALW
                     NKAGEAGMLCSHIPEEYGGMDADFLYSTVVIEEVGRLGLTGIGFSLHSDIVAPYILHY
                     GSEAQKQHYLPKLVSGEMVTAIAMTEPGAGSDLQGVKTTAVLDGDEYVINGSKTFITN
                     GFLADLVIVVAKTDPKAGAKGTSLFLVEANTPGFSKGKRLEKVGMKAQDTSELFFQDV
                     RVPKENLLGQAGMGFAYLMQELPQERLTVGVGALSSAEAALQWTLEYTRERKAFGKSI
                     GDFQNTRFKLAEMATEIQIGRVFVDRCLELHLQGKLDVPTAAMLKYWGTDLQCKVLDE
                     CVQLHGGYGYMWEYPVARAWADARVQRIYAGTNEIMKEIIARSL"
     gene            3389..4294
                     /locus_tag="VFF22_14310"
     CDS             3389..4294
                     /locus_tag="VFF22_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HZX18019.1"
                     /translation="MASLRYGKQQGLGLTPTFEIHLVSPDGQPVRSFSDVLIPVDGAL
                     EDADVIILPAFWDDFDALCQRHPQVLSWLKQRHAAGSAICGEATGVFWMAQAGLLDGK
                     EATTYWRFFNEFAERFPRVLLNQEKHLSDADNLYCAGGVTSACDLYIYLIERFCGASV
                     AQGVARDILYEVQRNYAPGRIGFGGQKLHHDMTILQIQQWLEDHFADKFRFEDVAREH
                     GMSIRNFMRRFQTATGDKPLHYLQRLRIETAKGLLSATRKSIKTISYEVGYDDASFFA
                     RLFRQHTELSPNQYRRQFQHKDGHA"
     gene            4405..5370
                     /locus_tag="VFF22_14315"
     CDS             4405..5370
                     /locus_tag="VFF22_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18020.1"
                     /translation="MRRTLIALLAACSFSAAAADNWKPFPYDQSAYDYSGDKLRQAWP
                     QLTRGFGSDYPFPDADWVVTMATRHPQALEKTVTANTGFTGKPEEAEVYAAKLQDVWR
                     LVFRGDFAQAKQQGLALGVGGQVPAMFAQVIYAMFLVPEQEEKHRLLEEVITYTDQAG
                     ELVQADVAAQFGRVYAKARLGEELSVPVVLKRGYTSQIPNELKALLAKQPQLPFALAL
                     YGGYEAGVIRKVGKMVGRMTYGVSTDNMEKYFSRSFQAQDDLPIGHYEYANALTYVYG
                     DDEQAKALQHLKLAVAIKPINAMEALEVAHAQKMLAQYKQKLAQN"
     gene            complement(5433..5609)
                     /locus_tag="VFF22_14320"
     CDS             complement(5433..5609)
                     /locus_tag="VFF22_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6435 family protein"
                     /protein_id="HZX18021.1"
                     /translation="MFGLFKSDPAKKLRKQYSAKLEEAMQAQRNGDIRSYSMLTEEAQ
                     ALWAQLEPLERAKQ"
     gene            complement(5716..6342)
                     /locus_tag="VFF22_14325"
     CDS             complement(5716..6342)
                     /locus_tag="VFF22_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007946628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZX18022.1"
                     /translation="MLADSIAVLDFETTGMSPAQQARATEIGVVIVEGGQIVARYQSL
                     MNSGAWVPPFIEQLTGISNAMLRTAPPAAQVMQEVAEFVGDRPLLAHNASFDQKFWDA
                     ELALIRRRRVQPFACSLLLSRRLLPMAPSHKLGNLNQWVGLPDTGKAHRALADAEMAA
                     NLTCFMAALLRERHGIADISHELLCNLQRVPAAKMSLALQKIRDQAFG"
     gene            complement(6346..6825)
                     /locus_tag="VFF22_14330"
     CDS             complement(6346..6825)
                     /locus_tag="VFF22_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HZX18023.1"
                     /translation="MKKIAVFADVQNLYYTVRQAYGCHFNYAALWADISQHGQIVEAY
                     AYAIDRGDQKQQQFQQILRNLGFTVKLKPFIQRSDGSAKGDWDVGITIDIMDAAPRVD
                     EVVLASGDGDFDLLLDKIRGSYGVEAVAYGVPGLTAQSLVRAASRYVPIDGALLLRN"
     gene            6916..7509
                     /locus_tag="VFF22_14335"
     CDS             6916..7509
                     /locus_tag="VFF22_14335"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HZX18024.1"
                     /translation="MHPRPANTPETPALLIIDMQQGIQRVSAPRNNPGAEACIAQLLE
                     YWRSADLPLVHIRHISRQADSVFAPGQSGALFQPALAPRATEQVFEKNVTDAFTHSGL
                     ERWLHARAIRRLVIVGVASENSVEATARSASNLGFITQVVADACYTFDKTDYYGQLRS
                     ADEVHAMAMANLRDEYAEVLTSADLLAALLGATSRIS"
     gene            7544..8206
                     /locus_tag="VFF22_14340"
     CDS             7544..8206
                     /locus_tag="VFF22_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HZX18025.1"
                     /translation="MNMLSALRDSWYFFSHNLAAIARLCLPLIVLEGLLLQQVTSLVA
                     AESATLWRLLAGLLFYPLYSAALILFLDARSQGLQPRARDLLAASLRLWPSFALLAGL
                     NTLAILLGASLFVLPGIWLMVKLAFAEYLLVLRGLSPMKALHESFQLSSGYFWPMLGC
                     ILIVMLPLWLLDGWSHSSETFADNALASLLLDCASRFLQLFSSVVLFRLFMLRSAQPE
                     VE"
     gene            8238..8672
                     /locus_tag="VFF22_14345"
     CDS             8238..8672
                     /locus_tag="VFF22_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZX18026.1"
                     /translation="MADANPSILSHISIGTNQFDKATAFYDQVLATLGCKRVLAHPGA
                     VAYGREYPEFWVQTPINGKPASIGNGSHFGFVAPDKAAVHDFFHAALEAGASDEGAPG
                     PRAEYGEPYYGCFVRDLDGHKIEAAFWDLEMVYEVYIEPHEH"
     gene            complement(8786..9631)
                     /locus_tag="VFF22_14350"
     CDS             complement(8786..9631)
                     /locus_tag="VFF22_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX18027.1"
                     /translation="MYRKVFANKVFDRKVVVITGGCAGIGRALAMRLAQAGARLVIFD
                     LQQQALDSLVQHLADHHNAEALGVLCDVADAAAVERAMALIVERFGGIDVLVNNAGIT
                     HRSSFADTELAVFQRIMAVNYFGALHCTKAALPSLIARSGQIIVLSSLSGFAPLLYRS
                     AYNSSKHALHGLFETLRYELKGSGVNVMLVCPGFTATDLRKNALVGDGSVAAQPPLAM
                     GKVASPQDVAEAIYHGALKRRRLLVLSNVDWRARVLARFFPRLFERVLLPRLSGLKPQ
                     RAGRS"
     gene            complement(9668..9907)
                     /locus_tag="VFF22_14355"
     CDS             complement(9668..9907)
                     /locus_tag="VFF22_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HZX18028.1"
                     /translation="MNSPTIAQRSPYAVAVIAGKDYFWCRCGRSAAQPFCDGQHKSSG
                     FTPLKYHADKDATLYFCGCKHTQTPPCCDGSHNRL"
     gene            10214..10957
                     /locus_tag="VFF22_14360"
     CDS             10214..10957
                     /locus_tag="VFF22_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX18029.1"
                     /translation="MPRFLCLVLAAALCCSTSFATPANDPRPLVQVGYYEFPPYSYTD
                     SQGRSKGAILQLSKRLLEHAGYSAELRSYPSARLYNGLQDGSVHVWPGAPGKVELRQH
                     TLETHTQLSEIVLNLYFRRDTLMPRLPEDLKGRGVIMISGYTYWQPVNEMLNDPQLAV
                     EQHRTGTHTAALEMLQRRRGDFLLDYQTPVEQARRRLGMSELPFVQLQRIPLKLIVSS
                     HAPGAEALRDALDRAYAELTAAGEDLRLP"
     gene            complement(10961..13495)
                     /gene="hrpB"
                     /locus_tag="VFF22_14365"
     CDS             complement(10961..13495)
                     /gene="hrpB"
                     /locus_tag="VFF22_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HZX18030.1"
                     /translation="MNSLPIDVLLPELCQALALRDEAVLEAPPGAGKTTRVPLALLTQ
                     PWLAGQTIIMLEPRRLAARAAAERLASELGEAVGETVGYRIRLDSKVGLRTRIEVVTE
                     GILARRLQDDPALEGVGLVIFDEFHERSLDADLALALCLNGRAMFRGADSGELPLKVL
                     LMSATLEGERLAGLLDDAPVLRSEGRMFPVDIRWGAPWQTGEWLEPKVLQCVLQALAD
                     ERGSLLVFLPGQAEIRRVNEQLAEALAGRRDILLCPLHGELDLSAQREAIEAAPPGLR
                     KVVLATNIAETSLTIDGVRVVIDAGLARVPRFDPNSGMTRLDTQRISRASATQRSGRA
                     GRLQPGVCYRLWSQAQHEQLAAYGTAEMLQADLAGLALQLVRWGVQPAELVWLDPPPA
                     AAYAQALELLGRLGAVDAQGVLTSHGQAMAELPTHPRIAHLLLRGQELGLGPLACDLA
                     ALLGERDILRGAGADLHSRLALLAGESRAARGAQGGVQRARQLARQFQGYLKRRPSPQ
                     AVSDPEHPRWLGALLAFAYPDRIAQQRRAGGAEYRLANGRAALFAEVDGLMKHAWLVV
                     ADLGSRQGQREERIYLAAELEPQLFEGVLAEQVSNLDVLGWDEREGALRAERQRKVGE
                     LVLSREALTGLDAQQRSRAMLGLVRRKGLALLPWTPELRQWQARVALLRALDLASKDA
                     SEWPDVSDAALLATLETWLQPYLDNVTRLSHFANLDLPSLLLNLLPWPLPQRLDELAP
                     RSVQVPSGSRISLDYSEQPPVLAVRLQELFGLADTPRIAGGRQVVKLHLLSPARRPVQ
                     VTQDLANFWRSTYAEVKKDLKGRYPKHYWPDDPLIAEPTARAKPRK"
     gene            13663..14082
                     /locus_tag="VFF22_14370"
     CDS             13663..14082
                     /locus_tag="VFF22_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZX18031.1"
                     /translation="MSTQTRVIGGLIVAALLTQLTACGTLFFPDRRGQIEGRIDPMVA
                     GLNAIGILFYVIPGLIAFGIDFATGAIYLPGGMTSQVDPQDLQNVVDADGKVDPIKLK
                     ALIEVQTGHSLPLDDPRLIQHSGSAEQLAAYGLRPAA"
     gene            14210..15085
                     /locus_tag="VFF22_14375"
     CDS             14210..15085
                     /locus_tag="VFF22_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZX18032.1"
                     /translation="MRLATFAALAAALTLALAKAIAWWLSGSVSLLAGLTDSLLDGAA
                     SLLNLIAVHYALRPADEDHRYGHGKAEALAGLGQALFIGASALLVGSHAIDRLLNPEP
                     LGAPALGIAVMLLSLAVTIALLLFQGHVVKLTGSTAIRADSLHYRSDLLLNSSILLAL
                     LLASYGWPQLDAIFGIGIALYIFWSAISIVREAATVLMDQELDPQISAQMHSLACAVP
                     GVLGVHDLRTRLSGTHWFVQLHVELPGELSLQHAHGLCDQVEAAIRSEFPRAEVLVHA
                     DPSSVVKHAPAPADH"
     gene            15483..15962
                     /locus_tag="VFF22_14380"
     CDS             15483..15962
                     /locus_tag="VFF22_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZX18033.1"
                     /translation="MNRLDRYDLNILAELQRDATLSNQELAERIGLSPSPCSRRVKQL
                     EDDGYILGQVALLDRKQLGLTLTAYVLIGMDKHTPERFEHFQEEIRKCPQVLECCLVT
                     GMDADYQLKVVVPDMDHYQKLLLGTLTRIEGVSSVRSSFVLQQILSSTQLPLQHLRG"
     gene            16102..16320
                     /locus_tag="VFF22_14385"
     CDS             16102..16320
                     /locus_tag="VFF22_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HZX18034.1"
                     /translation="METEQFESLMMYALVGGLMVFMFFIIWDLAKTSKAGRLGTAILF
                     LGLGLCLFAFLAKPIITYIIEASRGIHG"
     gene            16406..16798
                     /locus_tag="VFF22_14390"
     CDS             16406..16798
                     /locus_tag="VFF22_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HZX18035.1"
                     /translation="MSPTDRVMQSYGRCCASPDFFDSFYQHFLASSPEVREKFANTEM
                     NGQKLLLRQGILNLVMHARGMPDTKLRALGCSHSRAAMDIRPELYVLWQQALLQTIGE
                     HDQQYCNETRSAWNEVLDKGIAVIKAGY"
     gene            complement(16799..17368)
                     /locus_tag="VFF22_14395"
     CDS             complement(16799..17368)
                     /locus_tag="VFF22_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZX18036.1"
                     /translation="MAKAPPAESRLLVLNKPFDVLTQFNDEQGRATLKDFVDVPGVYP
                     AGRLDRDSEGLLLLTNDGGLQARIADPKHKLSKTYWVQVEGEPSEAQVQQLRDGVQLN
                     DGPTLPAEARLLDTPELWERNPPVRFRKSIPTAWLELVIREGRNRQVRRMTAAVGLPT
                     LRLVRVRIGPFSLDGLQPGQWREVPARLD"
     gene            17573..18607
                     /locus_tag="VFF22_14400"
     CDS             17573..18607
                     /locus_tag="VFF22_14400"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HZX18037.1"
                     /translation="MSSSESIVETFTGWAAKAPGAPLEPHSYDPGPLGAEEVEVAVEY
                     CGICHSDQSMIDNEWGNARYPFIPGHEVVGTVVRLGPQARGLKLGQRVGIGWYKGSCM
                     HCSSCMEGSHQLCGTVKPTIVGSNGGFADRLRSHWAWAVPLPDGLDPALVGPLFCAGS
                     TVFSPLLDFDVKPTDKVGVVGIGGLGHLALGFLNAWGCEVTAFTSSLSKQEEAKRLGA
                     HKVVASTDSAALKAIAGSLDFLLVTASADLDWNAMIGTLRGKGRLHFVGIVPSAIPVH
                     VFNLIPKQKSLSGSPVGSPSNMATMLEFCARHQILPQVEHFPMSKVNEAIDHLRAGKA
                     RYRVVLDASK"
     gene            complement(18668..20140)
                     /gene="amn"
                     /locus_tag="VFF22_14405"
     CDS             complement(18668..20140)
                     /gene="amn"
                     /locus_tag="VFF22_14405"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0046033 - AMP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HZX18038.1"
                     /translation="MNPCSDDFIVANTAEEAVDRLAALHLQATTGLSQALKRYLKERI
                     KPDAEQQSRFRYPELRLIYLCQGEVPTTVRAYAKVQVPGTYSVTVTHPAAFRKYLLEQ
                     LRPLMSDFTVRVEVGISQQNIPYPYVVEQGDELAGSGVTAAELARVFPSTDLSAATDG
                     TADGLYDWENTDPLPLALFDAARVDFSLRRLVHYTGSDWRHVQPWILLTNYHRYVDQF
                     IRHGLDMLVGESRFNRMVLPGNVVIERGMAEGEMQAIIENVIWHRYQMPAYHLQAEDG
                     HGITLVNIGVGPSNAKNITDHLAVLRPHCWLMIGHCGGLRQSQTIGDYVLAHAYMRRD
                     GILDRVLPPHIPLPALAEVQQALQEAAKLVTGEQGDELKKRLRTGTVLTYDDRNWELR
                     WAQERPLINLSRAVAVDMESGTIAAQGYRLRVPYGTLLCVSDKPLHSEIKLPGAAGAF
                     YERAVTQHLHIGITALDLLRNQLNSLHSRKLRSFDEPPFR"
     gene            complement(20224..20550)
                     /locus_tag="VFF22_14410"
     CDS             complement(20224..20550)
                     /locus_tag="VFF22_14410"
                     /inference="COORDINATES: protein motif:HMM:NF023903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3703 domain-containing protein"
                     /protein_id="HZX18039.1"
                     /translation="MTREQAFWQEYLLARAQRGIDPAAELRHLERAHILGQRYFSEHL
                     RSHLWMLAWAWRQRDWAEGLAQLLRIPGSLGSLLGLFPLGNPGTRRVGPLTPQALPED
                     LQALLK"
     gene            20659..21402
                     /locus_tag="VFF22_14415"
     CDS             20659..21402
                     /locus_tag="VFF22_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18040.1"
                     /translation="MPRALLTLVLCLLPSFLLADTLDGHYLTTLDGQPAEMHLRSQGQ
                     QVTGEYVEGATLRLQISGNFDGQLLSAQISEPKSGQVIANMNATYGNGVLNTHIAARN
                     PTTGATLERKALFHRQNLSAAVAAPSSNPSSNATRDPALIGTWVHEKIVNSGGANFAS
                     MTTQMILQLQGDGSVSQWTRSVAGGADWNYDSPGELQYSGRWQSNNGLLEVQMQGRGD
                     YQPAAYYRFSDQYLVTESNTGKLIWQRRQ"
     gene            complement(21407..22087)
                     /locus_tag="VFF22_14420"
     CDS             complement(21407..22087)
                     /locus_tag="VFF22_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HZX18041.1"
                     /translation="MHIEPEVVTGAKLFLSYATAAGAFGLTAKLALDSVRSNGGAAAL
                     ALRSLLTTALVFCFFEVFPHHAVGVSEVHLILGSTLLLLFGAGATAVGLALGLLLQGL
                     LFAQFDLPQYGMNVTTLLVPLWAISLLAKRIIAPGTAYVDLSYKQALALSTAYQGGIV
                     AWVGFWAFYGNGFSGENLAAVGSFGLAYMSVILIEPLVDLGVLAAAKTLSAYSRGPLF
                     NSRLHQPA"
     regulatory      complement(22142..22350)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(22440..22871)
                     /locus_tag="VFF22_14425"
     CDS             complement(22440..22871)
                     /locus_tag="VFF22_14425"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZX18042.1"
                     /translation="MQVSLFEQLQAWLAGDIGADYGISQLQAAAGLSGFSSQPPAVHF
                     NPLGGVHGGYTATLFDAALGLAVYSQACAEEVYVTASLQVNYLRPIRLAALPLRTEAR
                     VLQRDGRQVAAQATLHDAQGQLCANASAVFTCLRSAASGAT"
     gene            complement(22882..24534)
                     /locus_tag="VFF22_14430"
     CDS             complement(22882..24534)
                     /locus_tag="VFF22_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZX18043.1"
                     /translation="MNASQHAETHEVFNQVPPLDGANLYRVDLPLQEWTKRFGGGWAE
                     QRLDVYGALAGGELMVAGFLANENKPVFKSHDRYGHRADLVEFHPAYHQLMSAAIAHG
                     LPSMPWTDPRAGAQVARAAMSYLHSQAEAGSGCPLTMTFASVPALKLQADVAEKWLPK
                     ILSTQYDPRNLPIEQKTGATIGMAMTEKQGGTDVRANTTRAYPVGLGGPGQAYELVGH
                     KWFCSAPMCDAFLTLAYTTKGLTCFLLPRHRPDGTRNEFYIQRLKNKLGNWSNASSEV
                     EFRGALAWMVGEEGRGVPTIIEMVALTRFDCMIGSSALMRQALTQAAHHCAHRQVGGR
                     VLSEQPLMQNVLADLALESEAALALTLRMGKALDNPHDEQEDKFARLVTAVGKYWICK
                     RAPAMINEAAECMGGAGYVEDTILPRLYREAPVNSTWEGSGNVQCLDVLRALSKEPGV
                     LDALFVELGDGHGDARLKAHIAKLQADFRDTADIQYRARQLTEDVALALQAKLLLEAG
                     NASVSDAFIASRLGGQGGRVYGTLPRGLDVEALLARSTPHLI"
     gene            complement(24531..25199)
                     /locus_tag="VFF22_14435"
     CDS             complement(24531..25199)
                     /locus_tag="VFF22_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZX18044.1"
                     /translation="MAYRTTALRIDRDQALRERIISAALARVAEGGFAALTMQALAED
                     VGIATGSLYRHFSSKGSLAAEVFAVASQREVDALATTVRGPCSPVQRLAAGLERFAAR
                     AWESRRLAFALIAEPVDPEVDEQRLLYREAYAELFVELLQEGAAQGVFRVQQINLVAA
                     CLVGAIAEALVGPLSPPARAAREAGYPAPSLAEVSQALVTFCLRAVGAPLLAPLPKKE
                     EPQP"
     gene            complement(25418..27799)
                     /locus_tag="VFF22_14440"
     CDS             complement(25418..27799)
                     /locus_tag="VFF22_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HZX18045.1"
                     /translation="MHYILLCFFMLVPGLAGALVFDENTRSLPLGKAMSVFEDVRGDA
                     SIEDITSPALQASFRQHDKGVLNAGYSRSVFWLRLDLEYRPQQATGSQPWLLELAYPP
                     LDHVQLYLPDEQGRYQLRQHTGDALPFSSREIKQHNYVFELQLPPAQRQSVYLRLESQ
                     GSIQAPLTLWAPNAYLEEQPARIYVLGIIYGVLLVMLIYNLFIFLSVRDTSYLYYILY
                     IASFGFYQLSVNGAAIEYFWPNSPWWANAATPFLIGAAALFGCQFARSFLHTAEHSPW
                     VDRALLLMMASGVLVMTLALTASYALSLRLATYLALGFTVVIFSAGILAWMRGMRVAR
                     YFIIAWTAFLVGGMINTLMVLGYLPNMFLTMYASQIGSALEVGLLSLALADRINAMKE
                     ERTRILQEAGLKLEAFNQELANSNRLKDEFLATVTHELRTPMNGVIGSLELMQMLEID
                     GELVHYHRTAASSARDMMRMVNDILALTELQAGRLYPRREPFSLRGLFDGLRAQYAPR
                     AEEKGLRFVLELDDSLPDTLEGDASKLVQSLGCLLDNAIKFTLHGEVRVRVTRGGTAI
                     DILPLRIEVIDTGVGFTVPADGKLYQRFQQLDGSMTREYGGLGIGLAICRQLVDLLGG
                     DLSHESQPGQGSCFRLELPLALPVQMTTPAAVVLRATGPALRMPEQCTVLIVEDNAIN
                     QLVTRGMLLKLGYQVRTADNGAEALEVLRNETIDAVLLDCQMPVMDGFATCRALRNLP
                     GCQLLPVLAITAHSHSGDRERCLAAGMSDYLAKPVKFEQLRSLLHDWVLCRAS"
     gene            28111..28905
                     /locus_tag="VFF22_14445"
     CDS             28111..28905
                     /locus_tag="VFF22_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HZX18046.1"
                     /translation="MKTPTSRPVVLCLSGHDPSGGAGLQADIEALLAQGCHAAPTVTA
                     LTVQDTVNVSDFRVLDREWVLAQANAVINDLPVEAVKLGMLGSVEMVETVLEIMQRLP
                     GIPLVCDPVLRAGGGGALGKDEVGYAMRERLLPVATIATPNLPEARILAELPDGSADE
                     CAEKLLPYIQHLLITGGHGDEHEVHNRLYSRDGSRHTFTCQRLPGSYHGSGCTLASTL
                     AGRLALGQELVSAVQFALDYTWRTLRDAEQPGHGQFIPRRLPLDAY"
     gene            28933..29559
                     /gene="thiE"
                     /locus_tag="VFF22_14450"
     CDS             28933..29559
                     /gene="thiE"
                     /locus_tag="VFF22_14450"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HZX18047.1"
                     /translation="MKLRGLYAITDSELLKGKLLPYVEAALKGGATLLQYRDKSSDDA
                     RRLREAEALRELCNRYGATLIINDDAELAARLGVGLHLGQGDGSLSVARALLWRQAII
                     GGTCHSQLELAEAAAKEGASYVAFGRFFNSNTKPGAPAADVALLAAAKAKVNLPIVAI
                     GGVTLDNAPELIAHGASMVAVVHGLFGADSAADVERRARAFGALFTQI"
     gene            29689..30978
                     /gene="hemL"
                     /locus_tag="VFF22_14455"
     CDS             29689..30978
                     /gene="hemL"
                     /locus_tag="VFF22_14455"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HZX18048.1"
                     /translation="MSRSETLFANAQKHIPGGVNSPVRAFKSVGGTPLFFKHAEGAYV
                     TDEDDKRYVDYVGSWGPMILGHSHPEVLDAVRNQLQHGLSYGAPTAMETEMADLVCSL
                     VPSMEMVRMVSSGTEATMSAIRLARGYTGRDNIIKFEGCYHGHSDSLLVKAGSGLLTQ
                     GVPSSAGVPADFAKHTLTLPFNDLAAVEQMLSEVGSSVACIIVEPVAGNMNCVPPAPG
                     YLQGLREQCDKHGVVLIFDEVMTGFRVALGGAQAYYGVTPDLTTFGKIIGGGMPVGCF
                     GGKREIMHCIAPLGPVYQAGTLSGNPLAMAAGLTTLRLIKRPGFHAELTDYTSRMLQG
                     LQDRADAAGIPFVTTQAGGMFGLYFSGADDIVTFDDVMASDVERFKRFFHLMLDGGVY
                     LAPSAFEAGFTSIAHGDAELKITLDAAERAFAEMKKA"
     gene            30978..31643
                     /locus_tag="VFF22_14460"
     CDS             30978..31643
                     /locus_tag="VFF22_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18049.1"
                     /translation="MQYSTAFSDAVLALACLACLACAIAIGKARKHYGEANQPALFCA
                     LLGFLLPAAAAAVGVIRFGIDPSAQAAHLWLSQASSFLGLPLLGAAALALGRGWQWSR
                     ANWGRILLGLCAFFELFRQMNQLEDYRLLLNLATLLLIIYAGAVQWPKRAPLAAAIAV
                     VGLFLLAGLAVGTEGFIGSLRRVDLFHALLTPAYPLLAWLLLSLPGSAKHGSTEENPV
                     KTL"
     gene            31736..>31896
                     /locus_tag="VFF22_14465"
     CDS             31736..>31896
                     /locus_tag="VFF22_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010427222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HZX18050.1"
                     /translation="MKRTGRTLSLGCLLLLLPLLASASGNSLLIPALGSCSLNTAPEE
                     LPAALEACE"
BASE COUNT         6267 a        10425 c         9478 g         5726 t
ORIGIN      
        1 ccgaaaacag gcgctatcgg gggctcgggt cagcgcgtcc gtaatacggc aagattcttc
       61 ggcaggcggc gcatcacttc cactgcagaa agaggtgcgc aattatgcag attgcgcgga
      121 gaatttcaac acgattatgc caacccgctt tttcaaggag ctagcaatgg attaccgtca
      181 actcggccgc accgacatca aggtcagcag tctgtgcctg ggcaccatga cctggggcga
      241 acagaacagc tctagcgagg gcttcgccca gatcgaacgc gccaaggcct acggggtaaa
      301 tttcatcgat accgcagaga tgtacccggt gccgccgcgc gccgagactt acagcaagac
      361 cgaacagatc attggtgact acttcaagca gcgcggtgat cgtgccgatt gggtattggc
      421 cagcaagatt gccggcccag gcaacggtat cggctatatc cgcgacggcc agctcaaaca
      481 caatcgcgcg catatcactg cagcactgga tgccagcctc aaacgcctgc aaaccgattg
      541 gatcgacctg tatcagctgc actggcctga gcgcccgacc aactatttcg gtcagcttgg
      601 ctatcgccac caggacagcg acttcacgcc gctggaggaa accctcgaag cactggatga
      661 gcaggtcaaa gccggcaaga tccgccatat aggcctgtcc aacgaaacgc cctggggcac
      721 catgaaattc ctgcaactag ccgaacaacg cggctggccg cgcagtgtgt cagtccagaa
      781 cccctacaac ctgctcaatc gcagctttga agtgggtctg gcggaaatcg ccctgcgcga
      841 acaatgcggc ctgctcgcct attcgcccct ggccttcggc atgctcagcg gtaaatacga
      901 gaatggtgcg cgccctgccg gggcccgtat cagcctgttc agccgcttcg cgcgttacac
      961 caacccggag tcggagtcgg cctgctcgcg ctacgtcgcc ctggcccgcg agcacggcct
     1021 cgacccagcg cagatggccc tagcctttgt caccgcacaa ccatttgtca ccagcaatat
     1081 catcggtgcg accagcctgg aacagctcaa cagcaatctg gccagcagcg aaataacgct
     1141 cagcgccgaa gtaaaggctg gcattgaggc gattcaccaa gcccagccca atcccgcacc
     1201 ttaaaaaagc gacgcttgat cgcgtctggc gtcgcgtcgc gatgccagac gatcagccaa
     1261 gcgcgtgggc tccggcagac gatgacgcct tacacaggcc atcgtcagcg ccacggcact
     1321 atcaagactg actcgattac cgggcgagac tatcagcgga cgcactgcat ccttgctgcg
     1381 cagcacccag ccaattgcag aaccgcgccg atccagcaac ggcgcgcgcg cgccccgctg
     1441 ctgaggcaat tcagcatgac ttccggtaag aatctttttc gccacaccga tggtgggcag
     1501 gccactgact acaccgaggt gggcagcgat acccaaacct cgcggatggg caataccatg
     1561 cccatcgacg aagatcagat caggctcccg aggcaacccg gccaacgcgc taagcaatgc
     1621 gggcaactcg cgaaacgaca ataaaccggg cacgtagggc atgctcgtgg ggatacgcgc
     1681 gaccgactcg gccagcggct cgagactgtc ggcatccagc agaaccgccg ccgcacgcgt
     1741 gattgtgccg ccctcttcga agccgacatc cacacccgcg attacgcgca acggcgggta
     1801 atcgtcctcc agcctaacct gcgtcgccag atgcttttgc atctcgcggg ccgcagcagc
     1861 actgccatcc caacctgaaa agggcgcagc aacgagctca gaaagcgatg ccataacggt
     1921 ctccagacgt tttcgtcttg gagcgctggc accgcgccca agttcgccca atctttgtcg
     1981 catgctcgtt agcggcgaaa agacaacgct cctagagcga gcgggcgatg atctctttca
     2041 tgatctcgtt ggtgccggcg tagatgcgct gcacccgcgc atcggcccag gcgcgtgcca
     2101 ctgggtattc ccacatatag ccgtagccgc cgtgcagctg tacgcactca tcgagcacct
     2161 tgcactgcag gtcggtgccc cagtacttga gcatcgctgc agtcggtaca tccagcttgc
     2221 cctgcaggtg cagctccagg cagcggtcga caaacacccg accgatctga atttccgtgg
     2281 ccatttccgc cagcttgaag cgggtgttct ggaagtcacc aatcgacttg ccgaatgcct
     2341 tgcgctcgcg ggtgtactcc agcgtccact gcagcgccgc ttcggccgag gacagcgcac
     2401 cgacaccaac ggtcagacgc tcctgcggca gctcctgcat cagataggcg aagcccatgc
     2461 cggcctgccc cagcaggttt tccttgggca cacgtacatc ctgaaagaac agctcggagg
     2521 tgtcctgggc cttcatgccg accttttcca ggcgcttgcc cttggagaaa cctggcgtat
     2581 tggcttccac cagaaacagg ctggtgccct tggcgccggc tttcggatcg gtcttggcca
     2641 ccacgatcac taggtcagcg agaaagccgt tggtgataaa ggtcttcgag ccgttgatca
     2701 catactcatc gccatccagc acggcagtgg ttttcacccc ctgcagatcg gaaccggcgc
     2761 ctggctcggt catggcaatg gcggtgacca tctcaccgga caccagcttg ggcaggtaat
     2821 gctgtttctg cgcttcgctg ccgtaatgca ggatgtaagg cgcaacgatg tccgaatgca
     2881 gggagaaacc gatgccggtc aggcccaaac gaccgacttc ctcaatcacc acggtgctgt
     2941 aaaggaagtc cgcgtccatg ccgccgtatt cttccgggat atgcgagcag agcatccccg
     3001 cttcccccgc cttgttccac agcgcgcggt cgatatgacc gtccttttcc cactgctgat
     3061 ggaaaggcac ggcttcctgc tcaaggaact tgcgcacgct gtcgcgaaac tgttcgtgtt
     3121 cggagctgaa aatcgttctg gggatcatgg caacacctgc ttgatagagc ggttttaaag
     3181 tggcctcgac tttagccaag caaacgcttg gtcacactgg acacaagcgc caaaaaataa
     3241 gacgatccag ccaactcatg accacttagc cttaataaga acaatatcta tgcctattca
     3301 gccaccagcg ccgctgcacc gcgtcagcat tcttgccacc gaaggtgtgt ttgcctcgac
     3361 tttgatgcag gccaaggact tcttccacat ggccagcctg cgctacggca aacaacaggg
     3421 cctgggcctg acgccgacct tcgaaatcca cctggtcagc ccggacggcc agccggtgcg
     3481 cagtttcagt gacgtgctga ttccggtcga tggcgccctg gaagatgccg acgtgataat
     3541 cctgccggct ttctgggacg acttcgacgc gctgtgccag cgccacccac aagtgctcag
     3601 ttggctgaaa cagcgccacg ccgctggcag cgcgatttgc ggcgaagcca ccggggtgtt
     3661 ctggatggcc caggccggcc tgctcgacgg caaggaagcg accacctatt ggcgcttctt
     3721 caacgagttt gccgagcgct tccccagggt gctgctcaat caggaaaagc acctgtcgga
     3781 tgccgacaac ctgtattgcg caggtggcgt gacctcagcc tgcgaccttt acatttacct
     3841 gatcgaacgc ttctgcgggg ccagcgtagc ccagggcgtg gcccgcgaca ttctttatga
     3901 agtgcagcgc aactacgccc ccgggcgcat cggctttggc gggcagaagt tgcaccacga
     3961 catgaccatc ctgcagatcc agcaatggct ggaagatcac ttcgccgaca aattccgctt
     4021 cgaagatgtc gcccgcgagc acggtatgag catccgcaac tttatgcgtc gcttccagac
     4081 cgccaccggc gacaaaccgc tgcattacct gcagcgcctg cgcatcgaaa ccgccaaggg
     4141 cctgctcagc gctacccgca agagcatcaa aaccatcagc tatgaagtgg gttacgacga
     4201 cgccagtttc ttcgcccgat tgtttcgtca gcacacggaa ctgtcaccca accagtaccg
     4261 ccgccagttc cagcacaagg acggccatgc ctgagagcag gcactgttac cgccgctggt
     4321 tctgcactga tccttggatg ggtaggtggg cacagagcgc caccctatga tcggcacact
     4381 gataacaaca acaggttgac cgccatgcgc cgtactctta ttgccctact cgccgcctgc
     4441 agcttcagcg ctgcagccgc ggataactgg aaacccttcc cttacgacca gagcgcctac
     4501 gactactcgg gcgacaaact gcgccaggcc tggccgcagc tgacccgagg tttcggcagc
     4561 gactacccct tcccagacgc cgactgggtg gtgaccatgg ccacccgcca tccgcaggcg
     4621 ctggagaaaa ccgtgacggc caacaccggc tttaccggca aaccggaaga agccgaggtc
     4681 tacgcagcaa aactgcagga cgtctggcgg ctggtatttc gcggcgactt cgcccaggcc
     4741 aagcagcaag gtctggccct gggggtcggc ggacaggtcc cggcgatgtt cgctcaggtg
     4801 atctacgcca tgtttctcgt gcccgagcag gaagaaaagc accgcctgct ggaagaagta
     4861 atcacctaca ccgaccaggc cggcgaattg gtccaagccg acgtagccgc acagtttggc
     4921 cgcgtctatg ccaaagcgcg cttaggcgaa gaactgtcgg tgccggtggt actcaagcgc
     4981 ggctacacct cgcagatccc caacgaactc aaagccctgc tggccaaaca gccacagctt
     5041 cccttcgccc ttgccctata tggtggctat gaagctgggg tgatccgcaa ggtcggcaaa
     5101 atggtcggcc ggatgaccta cggggtcagc accgacaata tggagaagta tttcagccgc
     5161 agtttccagg cccaggacga cctgcccatc ggccactacg agtacgccaa cgccttgacc
     5221 tacgtttacg gcgacgacga acaggccaag gccctgcagc acctcaagct cgccgtagcg
     5281 atcaagccga tcaacgccat ggaagccctg gaagtggccc acgcacaaaa aatgctcgcc
     5341 cagtacaagc agaaactggc gcaaaactaa gccccgttga aacgcaaaaa ggctgcctga
     5401 tggcagtctt tattcattct tgcatcggca atttattgct tggcccgctc caggggctcc
     5461 aactgtgccc acaacgcctg cgcttcttcc gtcagcatcg aatagctacg gatatcaccg
     5521 ttgcgctgcg cctgcatcgc ctcttccaac ttggcactgt attgcttgcg tagtttcttg
     5581 gcgggatcgc ttttaaacaa accgaacatg ctgtgtgcct ctgtcgacaa agagccccgg
     5641 ataggtgcct gcccagctta attcagccac gcgccatggt tgaggcgcag atgtaacaag
     5701 tcgctaaaag cgccttcagc caaaggcctg atcgcgaatt ttctgcaatg ccaacgacat
     5761 cttcgccgcc ggtacccgct gcaggttgca cagcagttca tgagatatat cggcaatgcc
     5821 atgccgttca cgcagcaacg ccgccataaa gcaggtcaag ttggccgcca tttccgcatc
     5881 agccagtgcg cggtgcgcct tgccggtatc gggcaggccg acccactggt tgagattgcc
     5941 cagtttgtgg ctgggtgcca tgggcaacag gcggcgtgac aacagcagtg aacaggcaaa
     6001 tggctgcacc cgccgacggc ggatcagcgc cagctcggcg tcccagaact tctggtcgaa
     6061 actggcgtta tgcgccagca acgggcgatc accgacaaac tccgccactt cctgcatcac
     6121 ctgcgcggcg ggcggcgcag tacgcaacat ggcgttgctg atgccggtga gttgctcgat
     6181 aaacggcggc acccaggcac cgctgttcat caggctctga tagcgggcga cgatctgccc
     6241 gccctcgaca atcaccacgc caatctcagt cgcgcgggct tgctgggccg gcgacatacc
     6301 ggtggtttcg aagtcgagta cggcgatgga atcggcaagc atcgatcagt ttctcaacag
     6361 cagtgcgccg tcgatcggca catagcgact ggcagcccgc accagtgatt gcgcggtcag
     6421 ccccggcacg ccataggcca ctgcctcgac gccataactg ccacgaattt tgtccagcaa
     6481 gagatcaaag tcgccatcac cggaggccaa caccacttcg tcgacccgcg gcgcggcgtc
     6541 catgatgtcg atggtgatgc cgacatccca atcgcccttg gccgagccat cgctgcgctg
     6601 gataaacggc ttgagcttca cggtaaagcc gaggttgcgc aggatctgct gaaactgctg
     6661 ctgcttctga tcaccgcgat caatggcgta ggcgtaggct tcgacaatct gcccatgctg
     6721 gctgatatcg gcccacagcg cggcgtagtt gaaatggcaa ccataggcct ggcgcacggt
     6781 gtaatagagg ttctgtacat cggcgaatac ggcgatcttc ttcaccggta atcctcatca
     6841 tgcggctgac gggcagaaag ccctcaggtt aaagtcagcg cgcagtatgc cagccaacag
     6901 ccatggacgg tcgaaatgca tccacgcccc gccaatacgc cagaaacccc agcactgctg
     6961 attatcgata tgcagcaagg catccagcgc gtcagcgcac cgcgtaacaa cccgggcgcc
     7021 gaagcctgca tcgctcagct gctggaatac tggcgcagtg cggacctgcc gctggtgcat
     7081 atccgccata tttcccgtca agccgactcg gtgtttgccc cgggacaaag tggcgcactg
     7141 tttcaaccgg cattggcgcc acgggctacc gagcaggtgt tcgaaaagaa cgtcaccgat
     7201 gcttttaccc atagcggcct cgaacgttgg ctacatgccc gcgctatccg ccgtctggtg
     7261 atagtcgggg tggccagtga gaactcggtc gaagccaccg ctcgcagcgc cagcaatctg
     7321 ggctttatca cccaagtggt ggcggatgcc tgctacacct tcgacaagac tgactactac
     7381 gggcagctgc gcagcgccga tgaggtgcac gccatggcca tggcgaactt gcgcgatgaa
     7441 tatgccgaag tgcttaccag cgccgatctg cttgccgcgt tgctgggtgc gacaagcagg
     7501 atcagctaga gtgcgagcct gaattcgagt acgacttgcc cccatgaata tgctctccgc
     7561 cctgcgcgac tcctggtatt tcttcagcca caacctggcg gcgattgccc gcctgtgcct
     7621 gccgctgatc gtgctcgaag gcttgttgct gcagcaggtc accagcctgg tggccgccga
     7681 atccgccacc ctctggcgcc tgctcgccgg cctgctgttc tacccgttgt atagcgccgc
     7741 gctgatcctg tttctcgacg cacgcagcca gggcctgcag ccgcgcgccc gcgatctgct
     7801 ggccgccagc ctacggctgt ggccaagttt cgccctgttg gcggggctca atacgttggc
     7861 gattctgctc ggcgcgtcgc tgttcgtgct gccgggaatc tggctgatgg tcaaactggc
     7921 atttgccgag tatttgctgg tgttgcgcgg cctgagcccg atgaaggcgc tgcacgagag
     7981 cttccaactg agcagcggtt acttctggcc catgctcggt tgcatcctga tagtcatgct
     8041 gccgctgtgg ctgctcgacg gctggagcca ctccagcgaa accttcgccg acaacgccct
     8101 ggccagcctg ctgctggact gcgcaagtcg ctttttacaa ctattctcaa gcgtagtgct
     8161 gtttcgtcta tttatgctgc gcagcgccca gcccgaagtc gagtgaacgc cagcgacaac
     8221 ccaaggagga ccgtcacatg gctgacgcca accccagcat cctttcgcat atctccatcg
     8281 gcaccaatca gttcgacaag gccacggcct tctacgatca ggtcctcgcc accctcggct
     8341 gcaagcgcgt actcgcccac cctggcgcag tcgcctacgg ccgcgaatac ccggaatttt
     8401 gggtacagac gccgatcaac ggcaaaccgg caagcatcgg caatggcagc cacttcggtt
     8461 tcgtcgcacc ggacaaagca gccgtacatg actttttcca tgcagccctg gaagccggcg
     8521 ccagtgacga aggcgcaccc ggcccacgcg cagaatatgg cgagccctac tacggctgct
     8581 ttgtccgcga tttagatggg cacaaaatcg aggcggcgtt ctgggacctg gaaatggtct
     8641 acgaggtgta tatcgagccg cacgagcact gattgaagct ctgggcgtgg accgggggtt
     8701 ttcgtcagac agatcatcaa gtcggccttg ccgttctgca gcggcgcccc aaaccacagc
     8761 agcagtctga gccaggcgcg gtgcatcagg aacgccctgc acgctgcggt ttcaagccgg
     8821 ataggcgcgg cagcagcacc cgctcgaaca gccgcgggaa gaaccgcgcc agcactcgcg
     8881 cgcgccagtc gacgttggac agcaccagca gtcgacggcg tttcaacgcg ccgtggtaaa
     8941 tcgcctcggc aacatcctgc ggcgaggcca ccttgcccat cgccaacggc ggctgcgcgg
     9001 ccaccgagcc gtcgccaacc agggcgttct tgcgcaaatc ggtggcggta aagccggggc
     9061 acaccagcat cacattgacc ccggagcctt tcagctcata acgcagggtt tcaaacagcc
     9121 catgcagggc atgcttgctg gagttgtagg cgctgcgata aagcaacggg gcgaagcctg
     9181 acagcgagct gagcacgatg atctgcccac tgcgggcgat caggctgggc agcgcggcct
     9241 tggtgcaatg cagcgcgcca aaatagttga ccgccatgat ccgctgaaac accgccagct
     9301 cggtgtcggc aaaactgctg cggtgggtaa ttccggcgtt attgaccagc acatcgatgc
     9361 cgccgaaacg ctcgacaatc agcgccatgg cgcgctccac cgccgcagca tcggccacat
     9421 cgcagagcac accgagggcc tcggcgttgt ggtgatcggc caggtgctgc accagactgt
     9481 cgagcgcctg ctgctgcaga tcgaagatca ccaggcgcgc gcccgcctgt gccaaacgca
     9541 tggccagcgc ccggccgatg ccggcacagc cacccgtaat caccacaacc ttgcggtcga
     9601 ataccttgtt ggcgaacacc ttgcggtaca taacctgccc ccgacccagt tactgcaaaa
     9661 gccgttacta caacctgtta tggctgccat cgcaacaggg cggcgtctgg gtgtgcttgc
     9721 agccacagaa gtacagggta gcgtccttat ctgcgtggta tttcaacggg gtaaaaccgc
     9781 tgcttttatg ctgcccatcg cagaagggct gtgcggcgct gcgtccgcag cggcaccaga
     9841 agtaatcctt gccggctatg accgcaaccg cataggggct gcgctgggca atcgttgggc
     9901 tattcatgcg acctccagaa gcatccgagc cagacgcggc cacatcggca tcgccagcaa
     9961 tctccggtat gctccctcag catagttcac ccgctggcga gcctgttgat agccctggcc
    10021 cggaccctcg acctgttcgc agcctaggga ttttccgacc atctgccggt gctcgcccgc
    10081 ctgttgctgc cgacgttgga ctaagctttt tcagcaagcc actggacaaa gctgctggca
    10141 gttagccatg ctcgccaaat gtgacacttt cataacgaaa agacggcaga tagccgcagt
    10201 acgtacccat ttgatgccac gatttctttg cctagtgctg gctgctgccc tctgctgcag
    10261 caccagcttc gccactcccg ccaatgatcc acgaccattg gtgcaggtcg gttattacga
    10321 atttccaccc tattcctaca ctgacagcca aggccgttcc aagggcgcca tcctgcagct
    10381 gagcaagcgc ctgctggagc acgccggcta cagtgccgag ctgcgctcct accccagcgc
    10441 acggctgtac aacggcctgc aggatggcag cgtgcacgtc tggccggggg cgccgggcaa
    10501 agtcgaactg cgccagcaca ccctggaaac ccacacccag ctcagcgaaa tcgtgctcaa
    10561 cctgtatttc cgccgcgata ccctgatgcc gcgcctaccc gaggacctca agggccgcgg
    10621 ggtgatcatg atcagcggct acacctactg gcagccggtc aacgaaatgc tcaacgaccc
    10681 gcagctggcc gtggagcaac accgcaccgg cacccacacc gctgccctgg aaatgctcca
    10741 gcgtcgccgc ggcgattttc tcctcgacta ccagaccccg gtggaacaag cgcgcagacg
    10801 cctgggcatg tccgagctgc cgtttgttca gctgcaacgt attccgctga agctgatcgt
    10861 ctccagccat gcgccaggcg ccgaagcact gcgcgatgcg ctggatcgag cctatgcgga
    10921 actcacggcc gccggagagg atctgcgcct gccctaaagc ctacttgcgc ggtttggccc
    10981 gcgccgtcgg ttcggcgatc agcggatcat ccggccaata atgtttcgga tacctgccct
    11041 taaggtcttt ctttacctcg gcgtaggtgc tgcgccagaa gttggccaga tcctgggtaa
    11101 cctgcaccgg acggcgcgcg ggcgacagca ggtgcagctt gaccacctgg cggccgccgg
    11161 caatgcgcgg cgtatcagcc aggccgaaca gctcctgcag gcgcactgcc aacaccggcg
    11221 gctgctcgct gtaatccaga ctgatgcgcg aaccagacgg cacctgcacg ctgcgtggcg
    11281 ccaactcatc caagcgctgc ggcagcggcc agggcagcag gttcagcaac aggctgggca
    11341 aatccaggtt ggcgaaatgg ctgagccggg tgacgttatc cagatagggc tgcagccagg
    11401 tctccagcgt cgccagcaag gcagcgtcac tgacatccgg ccattcgctg gcgtccttgc
    11461 tggccagatc cagcgcgcgc aacaacgcca cccgcgcctg ccactggcgt aactccggcg
    11521 tccagggcaa cagcgccagg cccttgcgcc gaaccaggcc gagcatggcg cggctgcgtt
    11581 gctgtgcatc gagcccagtc aacgcctcgc gtgacagcac cagctcacca accttgcgct
    11641 ggcgttcggc gcgcagggcg ccttcgcgtt catcccaacc cagcacgtcc aggttgctga
    11701 cctgctcggc cagcacgcct tcgaacaact gcggctccag ctcggcggcc agatagatgc
    11761 gttcttcacg ctggccctgg cgactgccca ggtcagccac caccagccag gcgtgtttca
    11821 tcaggccatc gacctcggca aacagcgccg cccgtccgtt ggccaggcga tattccgcac
    11881 caccggcacg gcgctgctga gcgatgcgat ccgggtaggc gaaggccagc aacgcgccca
    11941 accaacgcgg atgctcagga tcactgactg cctgcgggct gggcctgcgc ttgaggtacc
    12001 cctgaaactg cctggccagt tgccgggccc gctgcacgcc accctgggcg ccacgggcgg
    12061 cccgcgactc acccgccagc aaagccaggc ggctgtgcag atcggcgccc gcaccacgca
    12121 gaatgtcgcg ctcgccaagc aaggccgcca gatcgcaggc caatggcccc aggcccagtt
    12181 cctgacctcg cagcagcaga tgggcgatac gtggatgagt gggcagctcg gccatggcct
    12241 ggccgtgact ggtgagcacg ccctgggcat ccaccgcgcc caaacgcccc aacagctcca
    12301 aagcctgggc ataggcggct gcaggtggcg gatcaagcca caccagctcg gccggctgca
    12361 cgccccagcg caccagttgc agggccaggc cggcgaggtc ggcctggagc atttccgccg
    12421 tgccgtaggc tgccaattgc tcatgctgcg cctgcgacca caggcgataa caaacgcccg
    12481 gctgcaagcg cccggcccga ccactgcgct gggtggcgga agcacgggaa atgcgctggg
    12541 tgtccaggcg ggtcatgcca ctgttcgggt caaaccgcgg cacccgtgcc agaccggcat
    12601 caatcaccac ccgcacgcca tcgatggtca ggctggtttc ggcgatattg gtcgccagca
    12661 ccaccttgcg caggcccggc ggagcggctt cgatggcctc gcgctgggcg ctgaggtcca
    12721 gctcgccatg cagcggacag agcagaatgt cgcgacgccc ggccagggcc tcggccagct
    12781 gttcattgac ccggcgaatc tccgcctgcc ccggcagaaa caccagcagg ctgccccgtt
    12841 catcggccag tgcctgcaat acgcactgca gcactttcgg ctcaagccac tcgcccgtct
    12901 gccagggcgc gccccagcgg atatccacgg ggaacatccg tccttcgctg cgcagcaccg
    12961 gcgcatcatc gagcaaaccc gccagccgct cgccttccag ggtcgccgac atcagcagca
    13021 ccttcagcgg cagctcgcca gagtccgcac cacgaaacat cgcccggcca ttcaggcaca
    13081 gcgccagcgc caggtcggca tccagactgc gctcgtgaaa ttcgtcaaag atgactaggc
    13141 cgacgccctc cagcgccggg tcatcctgca gccgccgcgc caggatgcct tcagtgacta
    13201 cctcgatacg ggtacgcagg ccaactttgc tgtccagacg aatgcgataa ccgacggttt
    13261 cccccaccgc ctcacccagc tcgctggcca ggcgttcggc cgccgcccgc gccgccagcc
    13321 gccgcggctc gagcatgatg atggtctgcc cggccagcca aggctgggtc agcagcgcca
    13381 gcggcacacg ggtggtcttg ccggcaccgg gcggcgcttc cagcaccgct tcatcacgca
    13441 gggcaagggc ctggcacaac tcaggcagca gcacatcgat tggcagggaa ttcatgcggg
    13501 ctccattcaa ggcgcaggat tataccggcg aacctgcgcg gcattggcgc tgtctcaata
    13561 gctggcattg cctcgcatga aatgcgccgg caacgcctga tgcagcggca ttgcaataca
    13621 ggcattggta tagttgcgcc acttttccca tggagatgtt caatgagcac ccaaacccgc
    13681 gttatcggtg gccttatcgt cgccgccctg ctcactcaac tgaccgcctg cggcaccctg
    13741 ttcttcccgg atcgtcgcgg ccagatcgaa ggccgcatcg accctatggt ggctgggctc
    13801 aacgccattg gtattctctt ctatgtaatt cccgggctga tcgcctttgg tatcgacttt
    13861 gccaccggcg cgatctacct gcccggcggc atgacctcac aggttgaccc gcaagacctg
    13921 cagaatgtgg tggatgccga cggcaaggtc gatccgatca agctcaaggc actgatcgaa
    13981 gtgcaaacgg gccacagcct gccactggat gacccacgcc tgatccagca cagcggcagc
    14041 gccgagcagc tggcggccta tggtctgcgc ccagcagcct aagcccggca ccagcaagcc
    14101 cgaactgaaa gcccgtttta cgacgggctt ttgttattta gcgctagcct tggcagtact
    14161 cgcccattct ggatagcccg tgaacataca gccgcaacac gcccgcctga tgcgcctggc
    14221 gaccttcgcc gcactggcgg cggcacttac gctggcactg gccaaagcca ttgcctggtg
    14281 gctcagcggc tcggtcagcc tgctcgcagg gctgacggac tcgctgcttg atggcgccgc
    14341 ctcactgctc aatctgatcg ccgtgcacta cgcgctgcgc ccggccgatg aggatcaccg
    14401 ctacggccat ggcaaggccg aagccctggc cggtttgggt caggcgttgt ttatcggcgc
    14461 cagcgcgttg ctggtgggca gtcacgccat cgaccgtctg ctcaatcctg aaccgcttgg
    14521 cgcaccggca ctgggcatcg cggtgatgct gctgtcgctg gcggtgacca tcgccctgct
    14581 gctgttccaa ggccatgtgg tcaagctgac cggctccacc gcaattcgcg ccgactcgct
    14641 gcactaccgc tcggacctgc tgcttaacag cagcatcctc ctggcattgc tgctggccag
    14701 ttacggctgg ccgcaactgg acgccatctt cggtatcggc attgcgctgt atatcttctg
    14761 gagcgccatc agcatcgtcc gcgaagcggc caccgtgctg atggatcagg aactcgaccc
    14821 gcagattagc gcgcagatgc atagcctagc ctgcgcggtg cccggcgtgc tgggcgtgca
    14881 tgacctgcgc acgcgcctct ccggcaccca ctggttcgtc cagctgcatg tggagctgcc
    14941 aggcgagttg agcctgcagc atgcccacgg cctgtgcgat caggttgaag cggcgatacg
    15001 cagcgaattc ccccgcgcag aggtactggt gcatgccgac cccagcagcg tggtaaagca
    15061 cgccccagcc cctgcagacc actagcgcgc aacaaaaaat tgcctggagc actttttgac
    15121 gccgcgcagc gacggcccgc agggtgtccg ccaaagatgg cagacacaaa aaaggggaag
    15181 gtctgcaggc cttccccttt tttgcgtgtc cgtcattgtg gcgatggtaa cgccttttct
    15241 tcgcctgcct ggttgggcag tgcggacccg ggtgccgtga cgatccggtg ggatcggcgg
    15301 cgaccgctca cctgattggg cgaggcggct ggacgtgtcg tccgggcctt gaagttgagg
    15361 taaagcttac gcccgccggc gcagtgaaag attgcgaaca ttgctgaaat acccaccact
    15421 tgcgcaatta atcaccacaa gcgcatcatc aagcacaacc aaataatgaa agcatggcaa
    15481 agatgaacag acttgatcgt tatgacctga atatcctcgc cgaattgcag cgcgacgcca
    15541 ccctgtccaa ccaagagctg gccgaacgta tcggcctgtc gccctcgccc tgttcgcggc
    15601 gagtcaaaca gcttgaagac gacggctata tcctcggcca ggtcgccctg ctcgaccgca
    15661 aacagctcgg cttgacgctg accgcttacg tgctgatcgg catggacaag cacacccctg
    15721 agcgtttcga gcattttcag gaagaaatcc gcaaatgccc gcaagtgctc gaatgctgcc
    15781 tggtcaccgg aatggacgcc gactatcagc tcaaggtggt ggtgccggac atggatcact
    15841 accagaaact gctgctgggc accctgaccc gtatcgaggg agtttccagc gtgcgttcca
    15901 gctttgtcct gcagcagatc ctctccagca cccagctgcc gctgcagcac ctgcgcggct
    15961 gatcgccgca ccctagccat gcgcggctgg cagggccgcc cccggcgcgt ataatcgcgc
    16021 ccctctggcg agcgtcaccg cgcccgccta cgcatcgatt gtcggcaccc agccgagcac
    16081 ctgcaggttc acgaggcccg aatggaaacc gaacagttcg aatccctgat gatgtacgcc
    16141 ctagtcggcg ggttgatggt gtttatgttc ttcatcatct gggacctggc gaaaacctcc
    16201 aaagccggcc gcctgggtac ggcgatcctg tttctcggcc tgggcctgtg cctgttcgcc
    16261 ttcctagcca agccgatcat cacctacatc atcgaagcaa gtcgcggcat ccacggctag
    16321 aaagctgccc cgcgccgact actgcacgtc cagccgcatc taaccgcgtt agaatgcggc
    16381 ttttcgccgt ttcctaggag tattcatgtc ccccaccgac cgggtgatgc agagctatgg
    16441 ccgctgctgc gccagccctg atttcttcga cagtttttac cagcacttcc tcgccagctc
    16501 accggaggtg cgcgagaagt tcgccaatac cgagatgaac gggcaaaagc tactgctgcg
    16561 tcagggcatt ctcaacctgg tgatgcatgc gcgcggtatg cctgacacca agctgcgcgc
    16621 cctgggctgc agccactccc gcgccgccat ggacattcgc ccagagctgt atgtgctgtg
    16681 gcagcaagca ctgctgcaga ccatcggcga gcatgatcag cagtactgca acgaaacccg
    16741 cagcgcctgg aatgaggtgc tggacaaagg catcgccgtt atcaaagccg gctactgatc
    16801 aatccagccg ggccggcact tcgcgccact gccccggctg caagccgtcc aggctgaacg
    16861 ggccgatacg cacccgcacc aggcgcagcg tcggtaggcc cacggccgcc gtcatacgcc
    16921 gcacctgacg gttgcgcccc tcgcgaatca ccagctccag ccaggccgtg gggatgcttt
    16981 tgcgaaagcg taccggcggg ttgcgctccc acaattcggg cgtgtccagt aaccgtgcct
    17041 cggccggcaa ggttggcccg tcattcaatt gcacaccatc acgcagctgc tgcacctgcg
    17101 cctcacttgg ttcaccctcg acctgcaccc aataggtttt agacagcttg tgcttggggt
    17161 cggcgatgcg cgcctgcaga ccgccgtcgt tggtcaacag caacaaacct tcgctatcac
    17221 ggtccagccg gcctgccgga tagacgccag gcacatcgac aaaatccttg agggtggcgc
    17281 ggccctgttc gtcgttgaac tgggtcagca catcgaacgg cttattcagc accagcaaac
    17341 gcgactcggc gggcggtgcc ttggccacac ggcgcacggc aggtttgccg ctgagcggac
    17401 gaggggacgc agggcgaggt gggcgcgaca taggtaatcc agcagcaggt tgcaaggcgg
    17461 ccagtgtaac cgaggccaag gttggactat caggctgagt gatccgcact gccttccccg
    17521 gcgtagcggg ctatgctggc taagttgtgt ttcccgtccc gaggccattg ttatgagttc
    17581 gtccgaatcc atcgtcgaaa cctttaccgg ctgggccgcc aaagcccccg gcgcgcccct
    17641 cgaaccgcac agttatgacc caggcccgct cggtgccgaa gaggtcgaag tggccgtgga
    17701 atactgcggc atctgccact ccgatcagtc gatgatcgac aatgaatggg gcaacgcgcg
    17761 ctaccccttt atccccggcc atgaagtggt cggcaccgtg gtacgcctcg gcccgcaggc
    17821 ccgcggcctc aagctcggcc agcgcgtggg catcggctgg tacaagggca gctgcatgca
    17881 ctgcagttcc tgcatggaag gctcgcacca gctctgcggc acagtaaaac cgaccattgt
    17941 cggcagcaac ggcggtttcg ccgaccgttt gcgcagccac tgggcctggg ccgtaccact
    18001 gccagacggc cttgatccgg cgctggtcgg cccgctgttc tgcgcaggct ccacagtgtt
    18061 tagcccgctg ctggatttcg atgtgaagcc caccgacaag gtcggcgtgg tcggcatcgg
    18121 cggcctcggt cacttggcgc tgggctttct caatgcatgg ggctgtgagg tcaccgcctt
    18181 cacttcatcg ctaagcaaac aggaagaagc caaacgcctg ggtgcgcaca aggtggtggc
    18241 ctccaccgac agcgcggcat tgaaagcgat tgccggcagc ctggatttcc tcctggttac
    18301 cgccagtgcc gacctggact ggaacgcgat gatcggcacc ctgcgcggca agggccggct
    18361 gcactttgtc ggcatcgtgc ccagcgcgat cccggtgcat gtattcaacc tgatcccgaa
    18421 gcagaagagc ctgtccggct cgcccgtcgg ttcgcctagc aatatggcga ccatgctgga
    18481 attctgcgca cgccatcaga tcctgccgca ggtcgagcac ttccccatga gcaaggtcaa
    18541 cgaggccatc gaccacctgc gcgccggtaa agcgcgctac cgcgtggtac tggatgccag
    18601 caaataagct gagctgaaat gaaaagcccg aggcgcaaac ctcgggcttt ttggtttcac
    18661 tggaagctta acggaacggc ggctcatcaa agctgcgcag tttccgcgag tgcagcgaat
    18721 tgagctggtt gcgcagcaga tccagcgcag tgatgcctat atgcagatgc tgggtcaccg
    18781 cacgctcata gaacgcaccg gccgcgcccg gcagtttgat ctcgctgtgc agcggcttgt
    18841 ccgagacgca cagcagcgtg ccgtagggca cccgcaggcg atagccttgc gcggcgatgg
    18901 tgccactttc catgtccacc gccacggcgc gggacaggtt gatcagcggc cgctcctggg
    18961 cccagcgcag ttcccagttg cggtcgtcgt aggtcagcac ggtgccggta cgcagacgct
    19021 ttttcagctc gtcaccctgc tcaccagtga ccagcttggc cgcttcctgc agagcctgct
    19081 gcacttcggc cagggccggc agtggaatat gcggcggcag cacgcggtca agaatgccgt
    19141 cgcggcgcat ataggcgtga gccagcacgt agtcgccgat ggtttgcgac tgacgcaggc
    19201 cgccgcagtg gccgatcatc agccagcaat gcgggcgcag cacagccagg tggtcggtga
    19261 tgttcttggc gttggacggg ccgacgccga tattgaccag ggtaatgccg tggccatcct
    19321 cggcttgcag gtggtaggcg ggcatctggt aacggtgcca gatgacgttc tcgataatcg
    19381 cctgcatctc gccttcggcc atgccacgct cgatcaccac gttgcccggc agcaccatac
    19441 ggttgaagcg cgactcaccg accagcatgt ccaggccatg gcggataaat tggtcgacgt
    19501 agcggtggta gttggtcagc agaatccatg gctgcacatg ccgccagtcg ctgccggtgt
    19561 aatgcaccag gcggcgcagg gagaaatcca cccgcgcggc atcgaacagc gccagcggca
    19621 gcgggtcggt gttttcccag tcgtacaggc catcggctgt accgtcggtg gcggcggaca
    19681 ggtcggtgct ggggaacacc cgcgccaatt cggcggcagt caccccggag ccggccagct
    19741 catcgccctg ttcgaccacg tacggataag gaatgttctg ctggctgatg cccacttcca
    19801 cacgcacggt gaagtcgctc atcagcgggc gcagctgctc cagcaggtat ttgcgaaacg
    19861 cagccgggtg cgtgacggtg acgctgtaag tgccgggcac ctgcaccttg gcataagcgc
    19921 gcacggtggt cggtacttcg ccctgacaca ggtagatcag gcgcagctcg gggtagcgaa
    19981 aacgcgactg ctgctcggca tccggcttga tacgctcctt gagatagcgt ttgagcgcct
    20041 ggctcaagcc cgtagtcgcc tgcaggtgca aggcggccag acgatccacc gcttcttcag
    20101 cggtgttggc gacaataaag tcatcggaac aagggttcac aaggcacctt cctggctgat
    20161 ctcaatggct ctatcttgcc ctgatccgcc gcgcttgcga tagcgcgacg ttgccggcgc
    20221 aggctacttg agcagcgctt gcagatcctc gggcagcgcc tgcggggtca gcgggccgac
    20281 gcgccgcgtg cctggattcc ctaagggaaa cagccccaga agcgaaccca ggctaccggg
    20341 gatgcgcagc agttgtgcca ggccctcggc ccagtcacgc tgtcgccaag cccaggcgag
    20401 catccacagg tggctgcgca ggtgctcgct gaaataacgc tgaccgagaa tatgcgcgcg
    20461 ctccagatgg cgcagctcgg cagcgggatc gatgccgcgc tgggcgcggg ccagtaggta
    20521 ttcctgccag aaggcttgtt cacgggtcat ggcaacactc ctctattaag ctgcgttgcc
    20581 agtctgcaag tacggggcgg cccccaggtc aagcatccgt tgctaatctt ctgtcagctc
    20641 atcttctaag gaaaactcat gccgcgcgcc ctgctcacac ttgtgctctg tctgttaccc
    20701 agttttctgc tggccgacac cctggacggc cactacctga ccacgctgga cggccagcca
    20761 gccgaaatgc acctgcgcag ccagggccag caagtcacgg gggaatatgt ggagggcgcg
    20821 acgctgcgcc tgcagatcag cggcaacttt gacggccaac tgcttagtgc gcagatcagc
    20881 gaaccgaagt ccgggcaagt gattgccaat atgaatgcca cctacggcaa tggcgtgctc
    20941 aacacccata tcgccgcacg caatccgact actggggcaa ccctggaacg caaggcgctg
    21001 ttccaccggc agaacctcag tgctgcagtc gccgcgccaa gtagcaaccc gagcagcaac
    21061 gcaacacgcg acccagcgct gatcggcacc tgggtacacg agaagatagt taacagcggc
    21121 ggcgccaatt tcgcctccat gaccacccag atgatcctgc aactgcaggg cgacggcagc
    21181 gtttcacaat ggacacgcag cgtcgcaggc ggtgcggact ggaactacga cagccctggc
    21241 gagctgcagt acagcggtcg ctggcagagt aacaatggct tgctggaagt acagatgcag
    21301 ggccgcggtg actatcagcc tgccgcctac tatcgcttca gtgaccagta tctggtcact
    21361 gagagcaata ccggcaagct gatctggcag cgtcgccagt gatcaatcag gccggttggt
    21421 gcagacgcga gttgaacaac ggcccacggc tataagccga cagcgtcttg gcggcggcca
    21481 gcacacccag atcgaccaat ggctcgatca ggatcacgct catataggcc agaccgaagc
    21541 taccgacggc cgccaggttc tcgccggaga agccgttgcc gtagaacgcc cagaagccga
    21601 cccaggcaac gatgccgccc tgataggcag tcgacagcgc cagcgcctgc ttgtaggaga
    21661 gatcaacata ggcagtgccc ggcgcaatga tgcgcttggc cagcaggctg atagcccaca
    21721 gcggcaccag cagcgtggtg acgttcatgc cgtactgcgg caagtcgaac tgagcaaaca
    21781 gcagcccctg cagtagcaaa ccgagcgcca ggccaaccgc cgtggcaccc gcgccgaaca
    21841 gcagcaacag ggtcgagccg aggatcaggt gtacttcgga aaccccgacc gcatgatgcg
    21901 gaaagacctc gaagaagcag aacaccaggg cagtggtcag caggctacgc aaagccaggg
    21961 cggcggcgcc accgttgctg cgtacgctgt cgagggcaag cttggccgtg aggccaaagg
    22021 cacctgcggc ggttgcataa ctgaggaaga gtttggcgcc agtgacgact tctggttcga
    22081 tgtgcataga gattcctgta ccgcgcccac cgcgcgatga tctggattga gggattgggc
    22141 ataagcgccc ggcttcgatg gcaggtctcc tggctcacgg tttgacactt cgcccgcctt
    22201 cccgaccttg tggccagtgg catgtgggcg tcattcaccg tttacagttg cgggggcagc
    22261 catggcttcg gcaccggttg tggtgctttc catgttccct tttcatccag tcgcgtagca
    22321 cgagctggaa ccatcggcgg ccaccttacc cggcggcaga gagaaatgac agagcccggc
    22381 cacgtgatga gtacacaccg gctgagcacg gcgcttcact ccaccggcat gcccaatagc
    22441 taggtggcgc ctgaggccgc actgcgcagg caggtgaata cggcgctggc gttggcgcac
    22501 agctgcccct gagcatcatg cagagtggct tgcgcagcta cctgtcgccc atcgcgctgc
    22561 agcactcgcg cttcggtgcg tagcggcaag gccgctaggc ggatcggacg caggtaattc
    22621 acctgcaggc tggcggtcac gtaaacctcc tcggcgcagg cctgcgagta caccgccaac
    22681 cccagcgctg catcgaacag cgtggcggtg tagccgccat gcacaccacc gagtggattg
    22741 aagtgcacgg ccggcggctg actgctgaac cccgacaaac ctgcggcagc ctgcagctga
    22801 gagataccgt agtccgcacc gatatcaccg gccagccacg cctgtaactg ctcaaagaga
    22861 ctgacttgca tactcggcgc ctcagatcag atgcggcgta ctgcgtgcca acagcgcttc
    22921 gacgtccagc ccgcgcggca acgtgccgta aacccgacca ccctgcccgc ccagacggct
    22981 ggcgataaag gcatcggaca ccgatgcatt gccggcttcc agcagcagct tggcttgcag
    23041 cgccagggcc acgtcttcgg tgagctgacg ggcgcggtac tggatatcgg cggtgtcgcg
    23101 gaagtccgcc tgcagcttgg cgatatgcgc tttcaggcgc gcatcaccgt gaccgtcacc
    23161 cagctcgaca aacagcgcat cgagcacacc gggctctttg gacagggcac gcagcacgtc
    23221 caaacattgc acgttgcccg agccttccca tgtggagttg accggtgctt cgcggtacag
    23281 ccgcggcagg atggtgtcct caacataccc ggcaccgccc atgcattcgg cggcttcgtt
    23341 gatcattgct ggcgcgcgtt tgcagatcca atatttgccc acggcggtaa ccaggcgggc
    23401 gaacttgtct tcctgttcgt catgcggatt gtccagcgcc ttacccatgc gcagggtcag
    23461 cgccagggcc gcctcgcttt ccagggccag gtcggccagt acgttctgca tcagcggctg
    23521 ctcgctcagt acgcggccgc caacctggcg atgggcgcaa tggtgtgcgg cctgggtcag
    23581 ggcctgacgc atcagcgcgc tggaaccgat catgcagtcg aagcgggtca gcgcgaccat
    23641 ctcgataatg gtcggcacgc cacggccctc ttcgccgacc atccaggcca gggcgccgcg
    23701 aaactccact tcgctggagg cgttcgacca gttgcccagc ttgtttttca ggcgctggat
    23761 atagaactcg ttgcgtgtgc catccgggcg gtgccgcggc agcagaaagc aggtcaggcc
    23821 cttggtggtg tacgccaggg tcaggaaggc atcgcacatc ggcgccgaac agaaccactt
    23881 gtggccgacc agctcgtagg cctggcccgg gccaccgagg cccaccgggt aggcacgtgt
    23941 ggtgttggca cgcacatcgg tgccgccctg tttctcggtc atggccatgc cgatggtcgc
    24001 accggttttc tgctcgatcg gcaggttgcg cggatcgtac tgggtcgaga ggattttcgg
    24061 cagccacttc tcggccacat cggcctgcag cttgagcgca ggcacgctgg cgaaagtcat
    24121 ggtcagcggg caaccgctgc cggcttcggc ctggctgtgc aggtaactca tggcggcgcg
    24181 cgccacctgg gcgccggcac gcgggtcggt ccagggcatg gacggcaaac catgcgcgat
    24241 ggctgcgctc atcagctggt gataggccgg gtgaaattcc accagatcgg cgcgatgacc
    24301 atagcggtca tggcttttga acaccggctt gttttcattg gcgagaaagc ccgcgaccat
    24361 cagctcacca ccggccagcg cgccatacac atccaggcgc tgctcggccc aaccgccgcc
    24421 gaagcgtttg gtccactcct gcagcggcag gtcaacgcgg tacaggttgg cgccgtccaa
    24481 cggcggcacc tggttgaaca cttcatgggt ttcagcgtgc tggctggcat tcatggctga
    24541 ggctcctctt ttttgggcaa tggggcgagc aatggggcgc ccacggcgcg tagacaaaag
    24601 gtaaccaggg cctggctgac ttcagccagg cttggggcgg gataaccggc ttcgcgggcc
    24661 gcacgcgctg gcggcgacaa ggggccgacc agggcctcgg cgattgcgcc gaccaggcag
    24721 gcagcgacca gattgatctg ctgcacgcgg aatacgcctt gagcggcgcc ttcctgcagc
    24781 aattcgacga acagctcggc ataggcttcg cggtagagca ggcgctgctc gtcgacttcc
    24841 ggatcaaccg gctcagcaat cagggcgaag gccaagcggc ggctttccca agcccgcgcg
    24901 gcaaagcgtt caagccctgc agccagacgc tgcaccgggc tgcaggggcc gcgcaccgtg
    24961 gtcgccaggg catcgacctc acgctggctg gccacggcaa acacctcggc cgccaggctg
    25021 cctttgctgc tgaagtggcg atacaggctg ccggtggcaa tgccgacatc ctcggccagc
    25081 gcctgcatgg tcaacgcggc aaagccgccc tcggccaccc gcgccagagc ggcgctgatg
    25141 atgcgctcac gcagcgcctg gtcgcggtcg atacgcaatg cagtggtgcg ataggccatg
    25201 gtctgaatcc tgattcacta attgaaatca gtgaatcagg attcagagtt tgcgcacaga
    25261 ggcatttcgg ccaagagagg gacgtttgtg cagcaggcat ccatagcccg gataaacctc
    25321 tgcatatgct tctcacagga gccagccccg gattgcgctg gcgcttattc gggctacgag
    25381 ggcggaggct aaggccaatt gtggctttac gctcgactca gctggcgcgg cagagcaccc
    25441 agtcgtgcaa cagactgcgc agctgctcga atttcaccgg cttggccagg tagtcgctca
    25501 taccggcagc caggcagcgc tcgcggtcgc cactgtggct gtgagcggtg atcgccagca
    25561 ccggcagcag ctggcaaccg ggcagattgc gaagcgcgcg gcaggtggca aaaccgtcca
    25621 tcaccggcat ctggcaatcc agcagcaccg cgtcgatggt ctcgttacgc agcacctcaa
    25681 gggcttcggc accattgtcg gcggtgcgca cctgataacc cagtttgagc agcatgccgc
    25741 gggtcaccag ttggttgatc gcgttgtctt cgacgatcaa taccgtgcac tgctcaggca
    25801 tgcgcagcgc cgggcctgta gcgcgtagca cgactgcggc tggggtagtc atttgcaccg
    25861 gcagggccag cggcagctcc agacggaaac agctgccctg gcctggctgg gattcatggc
    25921 tgagatcacc gccgagcagg tcgaccagtt gccggcagat cgccagaccg atacccaggc
    25981 caccgtactc acgggtcatg gagccatcca gctgctgaaa gcgctgatac agcttgccgt
    26041 cagctggcac ggtgaagccc acaccggtgt cgatcacctc gatgcgcagc ggcagaatat
    26101 cgatcgccgt gccgccgcga gtaacccgca cgcgcacttc gccatgcagg gtgaacttga
    26161 tggcgttatc cagcaaacag ccaaggcttt gcaccagctt gctggcgtcg ccctcaaggg
    26221 tatccggcag gctgtcgtcc aattccagaa caaaacgcag acccttttct tcggcgcgcg
    26281 gcgcgtactg cgcacgcaag ccgtcgaata ggccgcgcag actgaacggt tcacgccgag
    26341 gatataggcg cccagcctgc agctcggtga gggcaaggat gtcattgacc atgcgcatca
    26401 tgtcgcgggc cgagctggcg gcagtgcgat ggtagtgcac cagctcgccg tcgatctcga
    26461 gcatctgcat cagctccagc gaaccaatca ccccgttcat cggcgtgcgc agctcatggg
    26521 tgacggtggc gagaaattca tccttgaggc ggttgctgtt ggccagctcc tggttgaagg
    26581 cttcgagctt gagcccggcc tcctggagga tgcgcgtgcg ctcttccttc atcgcattga
    26641 tgcggtcggc cagtgctagc gaaagcaggc ccacttccag cgccgagccg atctggctgg
    26701 cgtacatggt gagaaacata ttcggcaggt agcccagcac catcagggtg ttgatcattc
    26761 cgccgacgag aaaggctgtc caggcgatga tgaaataacg cgccacacgc atgccgcgca
    26821 tccaggcgag gatgcccgcg ctgaagatca ccacggtaaa gcccagcgcc aggtaggtgg
    26881 ccagacgcag cgacagcgcg tagctggccg tcagcgccag agtcatcacc agcacgccgg
    26941 aggccatcat cagcaacagg gcgcgatcga cccaggggct gtgctcggcg gtatgcagaa
    27001 agctgcgggc gaactgacaa ccaaacaacg ccgccgcgcc aatcaggaaa ggcgtcgcag
    27061 cgttcgccca ccaggggctg ttcggccaga aatattcgat ggccgcaccg ttgaccgaca
    27121 gctgataaaa gccgaacgag gcgatataga ggatgtaata caggtagctg gtatcgcgca
    27181 cgctgaggaa gataaacagg ttgtagatca gcatcaccag cagcacgccg tagatgatgc
    27241 ccagcacata gatgcgcgcc ggctgctctt ccaggtaggc attcggcgcc cagagggtca
    27301 gcggcgcctg gatcgaaccc tgactttcca gacgcaggta aacgctttgc cgctgcgctg
    27361 gcggcagttg cagctcaaat acatagttgt gctgtttgat ctcgcgactg gagaacggca
    27421 aggcatcgcc agtgtgctgg cgcaactggt aacgcccctg ctcatcaggc aggtacagct
    27481 gcacatgatc aagcggcgga taggccagct ccagcaacca gggctgcgag cctgtcgctt
    27541 gctgcgggcg gtactccaga tccagacgta accagaacac cgagcgcgaa tagccggcgt
    27601 tcagtacgcc cttgtcgtgc tgacgaaaac tggcctgcag ggccggcgag gtgatgtcct
    27661 cgatgctggc atcgccgcgt acatcctcaa atactgacat ggccttgccg agcggcaggc
    27721 tgcgggtgtt ttcgtcaaaa accaatgccc cggccaaccc tggcaccagc atgaagaaac
    27781 agagaaggat gtagtgcatg caactcagcc atagtaagcc agcagcgcct taggtagcgc
    27841 cgctggaagc ggtgtaattg gatgctggca ctctagcata ggcccttgcg ccagagcccc
    27901 aggaaaaagc tgggcgcgca taatgcccct cacttaaacc acgttcacgg catttacgtg
    27961 tagcccgaat gtaatccgga aaccttctgg attccggaca accgtcccgg attatatccg
    28021 ggctacggca tgcgacagat cgtccgcccg actggctttc ctggcgtttc gaggtctgca
    28081 agccgtggtt tggtggtaag ctcgcgcacc atgaaaacgc ctacttcccg ccccgtagtg
    28141 ctgtgcctgt ccggtcatga ccccagtggt ggtgccggcc tgcaagccga tatcgaagcc
    28201 ctgctcgccc aaggctgtca cgccgctcca accgtgaccg cgctgaccgt gcaggacacc
    28261 gtcaatgtgt ccgacttccg cgtgctcgac cgcgagtggg tattggccca ggccaatgca
    28321 gtgatcaacg acctgccggt cgaagcggtc aagctcggca tgctcggctc ggtcgagatg
    28381 gtcgaaaccg tgctggagat catgcagcgc ctgccgggta tcccgctggt ctgcgacccg
    28441 gtactgcgtg ccggcggtgg cggcgcactg ggcaaggacg aagtcggcta cgccatgcgc
    28501 gagcgcctgc tgcccgtcgc caccatcgcc acgccaaacc tgccggaagc acgcatcctc
    28561 gctgagctgc cggacggcag cgccgatgaa tgcgccgaga aactgctgcc ctatatccag
    28621 cacctgctga tcaccggcgg ccatggcgat gagcacgaag ttcacaaccg cctgtacagc
    28681 cgcgacggca gccgccacac ctttacctgc cagcgcctgc ccggcagcta ccatggttcc
    28741 ggctgcaccc tggccagcac cctggccggt cgcctggcat tgggccagga attggtcagc
    28801 gccgtgcaat ttgccctcga ctacacctgg cgcaccctgc gcgatgccga acagcctggc
    28861 cacggccagt ttattccgcg ccgcctgccg ctggatgctt attaatagaa agccactgat
    28921 ggagcgtttg cgatgaaact gcgtggcctg tatgccatca ccgacagcga actgctcaag
    28981 ggcaagttat tgccctacgt tgaggccgca ctcaagggcg gtgcgacgct gctgcagtac
    29041 cgcgataaat ccagcgacga cgcccgccgc ctgcgcgaag ccgaagcgct gcgcgagctg
    29101 tgcaaccgct atggcgccac gctgatcatc aatgacgacg ccgaattggc cgcccgtttg
    29161 ggtgtaggcc tgcatctggg gcaaggcgat ggatcgctgt ccgtcgcccg cgccctgctc
    29221 tggcgtcagg cgattatcgg cggcacctgc cattcccaac tggaattggc cgaggccgca
    29281 gccaaggaag gcgccagcta tgtcgctttc ggccgcttct tcaattccaa caccaagccc
    29341 ggcgcaccgg ctgccgacgt cgcgttgctg gcggcggcga aagccaaggt caacctgccg
    29401 attgtcgcca taggcggcgt gaccctggac aacgcaccgg agctgattgc ccacggcgcc
    29461 agcatggtcg cagtggtgca cggcctgttc ggcgccgact cggccgctga cgtcgagcgc
    29521 cgcgcccgcg cctttggcgc cctgtttacc cagatataac ggcctggagc cattattaac
    29581 gtcgcacagc gatgacccga agggtggctg ccagggatgg caggccataa ttaattcttg
    29641 tcaggggcag cccagctcgc ccccactccc tgcttgagag gccctgccat gtcccgttcc
    29701 gaaaccctct tcgccaacgc gcaaaagcac atcccgggtg gcgtcaactc gccggtgcgc
    29761 gccttcaaaa gcgtcggcgg caccccgctg ttcttcaaac acgccgaagg cgcctacgtc
    29821 accgatgaag acgacaagcg ctatgtcgat tacgtcggct cctggggccc gatgatcctc
    29881 ggccacagcc acccggaagt tctcgacgca gtgcgcaacc agctgcagca cggtctgtcc
    29941 tacggcgcac cgaccgccat ggaaaccgag atggccgacc tggtatgcag cctggtgccg
    30001 tccatggaaa tggtgcgcat ggtcagctcc ggcaccgaag cgaccatgag cgccattcgc
    30061 ctggcccgtg gctacaccgg tcgcgacaac atcatcaagt tcgaaggctg ctaccacggc
    30121 cattccgaca gtttgttagt taaagccggc tccggcctgc tgacccaggg tgtaccgagc
    30181 tccgccggcg taccggctga tttcgccaaa cacacactga ccttgccgtt caacgacctg
    30241 gcagcggttg agcagatgct cagcgaagtc ggttcgagcg tcgcctgcat catcgtcgaa
    30301 ccggtggccg gcaatatgaa ctgcgtgccg ccagctccgg gctacctgca gggcctgcgc
    30361 gagcagtgcg ataaacacgg cgtggtgctg atcttcgacg aagtgatgac cggtttccgc
    30421 gtcgccctcg gcggcgctca ggcgtattac ggcgtcaccc cggacctgac caccttcggc
    30481 aagatcatcg gtggtggcat gccggtcggc tgtttcggcg gcaagcgcga aatcatgcat
    30541 tgcatcgccc cgctcggccc ggtgtaccag gccggcaccc tgtcgggtaa cccgctggcc
    30601 atggccgccg gcctgactac tttgcgcctg atcaaacgcc cgggcttcca cgccgagctg
    30661 accgactaca ccagccgcat gctgcaaggc ctgcaggacc gcgccgatgc ggccggcatc
    30721 ccgtttgtca ccacccaggc tggcggcatg ttcggcctgt atttcagcgg cgccgacgac
    30781 atcgtcacct ttgacgatgt gatggccagc gatgtcgagc gcttcaagcg cttcttccac
    30841 ctgatgctgg acggcggcgt gtacctggca ccaagcgcct tcgaggccgg tttcacctcc
    30901 atcgcccatg gcgatgccga gttgaaaatc accctggacg ccgccgagcg cgcctttgcc
    30961 gagatgaaga aagcctgatg cagtacagca ctgcctttag cgacgctgtt ctggcactgg
    31021 cctgtctggc ctgtctggcc tgcgcaatcg ccatcggcaa agcccgcaag cactatggcg
    31081 aggccaatca gccagcattg ttctgcgcct tgctgggatt tctcctgcca gctgcagcgg
    31141 cagccgtcgg ggtgattcgc tttggcatcg accccagcgc gcaggccgcg cacctgtggc
    31201 taagccaagc cagcagcttt ctcggcctgc cactgctcgg cgccgccgcg ctggctttgg
    31261 ggcggggctg gcagtggagc cgtgccaact ggggccgtat cctgctgggg ctgtgcgcct
    31321 tcttcgagtt gttccggcag atgaaccagc tggaggatta tcgcctgctg ctcaacctgg
    31381 cgaccctgct gctgattatc tatgcaggcg ccgtgcagtg gcccaaacgc gcgcccctgg
    31441 ctgcggcgat tgcggtggtc gggctgtttc tgctggccgg tttggcggtt ggcaccgagg
    31501 gctttatcgg ttctttacgc cgagtcgacc tgttccacgc cctgctgaca cccgcctacc
    31561 cactgctggc ttggctgctg ctaagcctgc ccggcagcgc aaaacatggc agcacggaag
    31621 aaaacccggt aaagactttg taagaacgcc gctgcttatt tcataatgtg atggctgccg
    31681 cgttccggcg gccaggcaga tcgcctgttt tgcatcccga ggtaaacgga ttttcatgaa
    31741 acgcaccggc cgcaccctgt ccctgggctg cctgttgctc ctgctgccac tgctcgcctc
    31801 ggcgagcggc aactccttgc tgatccccgc gcttggcagc tgctcactca acaccgcccc
    31861 cgaagaactg cccgccgccc ttgaggcctg cgagca
//
LOCUS       DASRUM010000215         5665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_446800, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000215 DASRUM010000000
VERSION     DASRUM010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446800"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1527)
                     /gene="rseP"
                     /locus_tag="VFF22_14470"
     CDS             complement(175..1527)
                     /gene="rseP"
                     /locus_tag="VFF22_14470"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HZX18051.1"
                     /translation="MSALYMLVGTLVALGVLVTIHEYGHFWVARRCGVKVLRFSVGFG
                     SPLLRWHDRQGTEFVLAAIPLGGYVKMLDEREGDVPPELLDQSFNRKSVRQRIAIVAA
                     GPVANFLLALLFFWVVAMLGSQQVRPVIGAVEAGSLADVAGLQAGQEIVAIDGQSTTG
                     WAAVNLQLVSRLGESGRLDLAVREPGSSVDSPRQIVLDNWLRGADEPDPIASLGIRPW
                     RPALQPVLAQLDSEGPAFAAGLQVGDRLLALDGQSLADWQQLVDQVRGLPGKKISLRV
                     ERQGQQLDVPLTLAARGEGEALSGYLGAGVAGGEWPPEMLREVSYGPLEAIAEGAKNT
                     WTMSVLTLNSLKKMLFGELSVKNLSGPITIAKVAGASAESGLSDFLKFLAYLSISLGV
                     LNLLPIPVLDGGHLLFYLVEWVRGRPLSERIQGWGMQIGISLVIGVMLLALVNDLGRL
                     "
     gene            complement(1549..2733)
                     /gene="ispC"
                     /locus_tag="VFF22_14475"
     CDS             complement(1549..2733)
                     /gene="ispC"
                     /locus_tag="VFF22_14475"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZX18052.1"
                     /translation="MSHLQQITVLGATGSIGLSTLDVIARHPERYQVFALSGFSRLTE
                     LEALCMIYRPRFAVVPQALQARHLQEGLRAAGLATQVLHGPEGLCEVAAHPEVDAVMA
                     AIVGAAGLPPTLAAVEAGKKVLLANKEALVMSGALFMQAVRKSGAVLLPIDSEHNAIF
                     QCLPGDYARGLPQVGVRRILLTASGGPFREMPLEQLHTVTPEQACAHPNWSMGRKISV
                     DSASMMNKGLELIEACWLFDATPAQIEVVVHPQSVIHSLVDYVDGSVLAQLGNPDMRT
                     PISHALAWPERIDSGVAPLDLFAIARLDFQAPDEQRFPCLRLAREAAQAGGTAPAMLN
                     AANEVAVAAFLERRIRFPEIASMIEDVLHTEPTLAVQNLDTVLAADGRARHLAEQWLS
                     RR"
     gene            complement(2730..3545)
                     /locus_tag="VFF22_14480"
     CDS             complement(2730..3545)
                     /locus_tag="VFF22_14480"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019472221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZX18053.1"
                     /translation="MLKQRIITALVLLPFALGGFFLLDGALFALFIGAVVSLGAWEWA
                     RLAGFTAQLQRVAYAALVAALLFGLYLLPALAPWVLLLGVVWWAVATFLVLGYPASSR
                     YWTQAPVRLLIGLLILLPAWQGLVLFKQWPEANWLILAVMVLVWGADIGAYFSGRRFG
                     KRKLAPQVSPGKSWEGVFGGLLATLLICLGVAIYRDWSFASLLLGLLGTAVVVLISVV
                     GDLTESMFKRQSGIKDSSNLLPGHGGVLDRIDSLTAAIPVFAALLWLAGWGAL"
     gene            complement(3545..4300)
                     /gene="uppS"
                     /locus_tag="VFF22_14485"
     CDS             complement(3545..4300)
                     /gene="uppS"
                     /locus_tag="VFF22_14485"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016392308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HZX18054.1"
                     /translation="MEKTKDGSPFAVPRHVAIIMDGNNRWAKKRLLPGVAGHKAGVDA
                     VRAVIEVCAEAGVEVLTLFAFSSENWQRPAEEVSALMELFLSALRREAKKLNANDISL
                     RIIGDRSRFHPELQAAMREAEAQTAGDKRFVLQVAANYGGQWDIAQAAQRLAREVQGG
                     HLRAEDITPELLQSCLVTGDLPLPDLCIRTGGEHRISNFLLWQLAYTELYFSDLFWPD
                     FKHDAMRKALADFASRQRRFGKTSDQVAAEARS"
     gene            complement(4317..4874)
                     /gene="frr"
                     /locus_tag="VFF22_14490"
     CDS             complement(4317..4874)
                     /gene="frr"
                     /locus_tag="VFF22_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZX18055.1"
                     /translation="MINEIKKDAQVRMQKSLESLLHAFSRIRTGQAHPSILGGVMVPY
                     YGSDTPLNQVANVTVKDSRTLQVVAFERNMLAAVDKAIQSSGLGFNPTNLGELLLISM
                     PALTEETRKGFTKQARDAAEDGRVAVRNIRRDALSQLKDLVKEKEISEDEERRAADEI
                     QKLTDKFVAEIEVAVKQKEADLMAV"
     gene            complement(4864..5613)
                     /gene="pyrH"
                     /locus_tag="VFF22_14495"
     CDS             complement(4864..5613)
                     /gene="pyrH"
                     /locus_tag="VFF22_14495"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZX18056.1"
                     /translation="MAQQMSGRQPRYKRILLKLSGEALMGSEDFGIDPKVLDRMALEV
                     GQLVGIGVQVGLVIGGGNLFRGAALSAAGMDRVTGDHMGMLATVMNALAMRDALERSN
                     IPAIVMSAISMVGVTDHYDRRKALRHLKTGEVVIFAAGTGNPFFTTDSAACLRAIEID
                     ADVVLKATKVDGVYTADPFKDPHAEKFERLTYDEVLDRKLGVMDLTAICLCRDHNMPL
                     RVFNMNKPGALLNVVVGGAEGTLIEEDKHDQ"
BASE COUNT         1282 a         1872 c         1517 g          994 t
ORIGIN      
        1 ggatatcgga gatggtgaag gactcggcgt gaacttcggt gatcatcagt gcggcaagca
       61 ccgcaggtag cagcagacgt ttcatgaagt cctttcttat tccaactgac aataaaaaat
      121 ctgccgctta gtgcgacacg tcttgtacgt accgagagcg taattcagca gcggctacag
      181 gcgacccagg tcgtttacca aggcaagcaa catcaccccg atgaccaaac tgatgccgat
      241 ctgcatcccc caaccctgta tccgttccga cagcgggcga ccacgcaccc actcgaccag
      301 ataaaaaagc agatgccccc catccaacac ggggataggc aacaaattaa gaacccccag
      361 actaatgctc aagtaggcga gaaatttaag aaaatcactc aaacctgact cagcagaagc
      421 gcccgccact ttagcaatgg ttatcggccc gctcaagttt tttaccgaga gctcgccaaa
      481 cagcattttc ttcagggaat taagggtcag aacgctcatt gtccaggtgt ttttggcacc
      541 ttcagcgatg gcttctaacg ggccatagct tacttcacgc aacatctcag gcggccattc
      601 gccacctgcg acaccagcac ccaggtaacc actgagcgcc tcaccttcac cgcgagcagc
      661 caaagtcagc ggcacatcca actgctgacc ttgacgctcc acccgcaagc taatcttttt
      721 acccggcaag ccacgcacct gatccaccag ctgctgccaa tcagccagag actggccatc
      781 gagcgcaagc aggcgatcgc caacctgcaa gcccgcagca aatgccggac cttcagaatc
      841 aagctgcgcc agcaccggct gcagcgccgg acgccaaggg cgaatcccca aagaagcgat
      901 agggtccggc tcatcggcgc cacgcaacca gttgtccagt acgatctgcc gggggctgtc
      961 gaccgaggag cccggttcgc gcacagccag atccagacga ccgctctcac ccaagcgact
     1021 gaccagttgc agatttactg cagcccagcc ggtggtggac tggccatcaa ttgcgacgat
     1081 ctcctgccct gcctgaaggc cggcaacatc ggccagactg cccgcctcga ccgcaccgat
     1141 aaccgggcgc acctgctggc tacccagcat cgccacaacc cagaagaaca gaagcgcaag
     1201 caggaaattc gccaccggcc cagccgccac gatagcaatg cgctggcgaa ccgacttgcg
     1261 attgaacgat tggtcaagca actccggcgg cacatcgcct tcgcgttcat cgagcatctt
     1321 cacgtagccg cccagcggga tggcggccaa aacaaactca gtcccctggc ggtcatgcca
     1381 acgcagcagc gggctgccaa agccgacaga aaaccgcagc accttgacgc cgcaacgacg
     1441 ggcgacccag aagtgtccgt attcgtgaat ggttaccagc accccaagtg caaccagggt
     1501 accgaccagc atataaagcg cactcatcaa acttctccgc gtcagcgatc agcgccgact
     1561 cagccattgt tcggccagat ggcgggcgcg gccatcagcg gccagcacgg tatccagatt
     1621 ctgaaccgcc agggtgggct cggtgtgcag aacgtcttcg atcatactcg cgatctcggg
     1681 aaagcggatg cgtcgctcaa gaaacgccgc aaccgccact tcattggccg cattgagcat
     1741 ggccggcgca gtaccgccag cctgcgccgc ctcacgcgcc aggcgcaggc aaggaaagcg
     1801 ctgttcgtcc ggcgcttgga agtccaaccg agcgatggca aacagatcca gcggcgcaac
     1861 acccgagtcg attcgctccg gccaggccag ggcgtgactg atcggcgtac gcatatccgg
     1921 gttacccagc tgcgccagca ccgaaccgtc gacataatcg accagcgaat ggatcacgct
     1981 ctgcggatgc accaccactt caatctgcgc tggcgtggcg tcaaacaacc agcaggcctc
     2041 gatcagctcc aggcctttgt tcatcatgct cgccgagtcg accgagatct tgcgccccat
     2101 cgaccagttg ggatgcgcgc acgcctgctc gggagtgacc gtgtgcaact gctccagcgg
     2161 catttcgcga aacggcccac ccgaggcggt cagtagaata cgtcgtacac cgacttgcgg
     2221 caggccgcgg gcgtagtcac ccggcaggca ctggaaaatc gcattgtgct cactatcgat
     2281 cggcagcaat acagcgccac ttttacgcac agcctgcata aatagcgcgc ccgacatcac
     2341 caaggcttct ttgttggcca gcagcacctt cttgccggcc tcgaccgccg ccagagtcgg
     2401 tggcaagcct gctgcgccga cgatggcagc catcaccgca tccacttccg ggtgtgccgc
     2461 cacttcacac agcccctcgg ggccatgcag cacctgagtg gctagaccgg cagcgcgcaa
     2521 accttcctgc agatggcgag cctgcagagc ctgcggcacc accgcaaaac gcggacggta
     2581 gatcatgcac aacgcctcta gctcagtcag acgactgaaa ccggacaacg cgaatacttg
     2641 atagcgctca ggatgacgcg cgattacatc gagggtgctc agaccaatcg aaccggtcgc
     2701 ccccagcaca gtaatctgct gcaggtgact cacagcgcgc cccagcccgc cagccacaac
     2761 agcgcggcga acaccgggat cgccgccgtc aggctatcga tacgatccag cacaccgcca
     2821 tggcccggca gcagattgct gctgtcctta atcccggatt ggcgcttgaa catgctttcg
     2881 gtcagatcgc cgaccaccga gatcaacacc actacggcgg tgccgagcaa gcccagcagc
     2941 aggctggcaa acgaccaatc gcggtaaatc gccaccccca ggcagatcag cagcgtcgcc
     3001 agcaggccgc cgaacacccc ttcccagctt ttaccgggac tgacctgcgg cgccagcttg
     3061 cgcttgccga agcgccggcc ggagaaatag gcgccgatat ccgcacccca caccaacacc
     3121 atcaccgcca gaatcagcca gtttgcttca ggccactgct tgaacagcac caagccctgc
     3181 cacgctggca gcaaaataag caaaccgatc agtaatctga ctggagcctg cgtccagtaa
     3241 cggctgctgg cgggataacc caacaccaga aaggtcgcca ccgcccacca gacaaccccg
     3301 agcaacaaga cccaaggcgc caaggcgggc agcaaataca ggccgaacaa caacgcagcc
     3361 accagcgctg cgtaggccac gcgttgcaac tgcgcagtaa aacctgccag gcgcgcccat
     3421 tcccaggcac ccagactgac cactgcgccg ataaacagcg caaacagcgc gccatcgagc
     3481 agaaagaagc cacccaaagc gaatggcaac agcaccagcg ccgtgataat tcgttgtttg
     3541 agcattagct acgagcctca gcagccacct gatcgctggt tttcccaaag cgacgctggc
     3601 ggctagcaaa atcggccaag gctttgcgca tggcgtcgtg tttgaaatcc ggccaaaaca
     3661 ggtcggagaa atacagctcg gtgtaggcca gctgccagag caggaaatta ctgatgcggt
     3721 gttcgccacc ggtgcggatg cacagatccg gcagaggcaa gtcgccggtc accaggcagc
     3781 tctgcagcaa ttcaggagtg atgtcctcgg cgcgtaaatg accgccttga acttcacggg
     3841 ccaaacgctg cgccgcctga gcgatatccc actggccgcc atagttggcc gccacctgca
     3901 agacaaagcg tttgtcgcca gcagtctgcg cttcagcctc acgcatcgcg gcctgcagtt
     3961 cagggtgaaa gcgtgagcga tcaccaatga tgcgcaggct gatgtcattg gcattgagct
     4021 tcttcgcctc gcggcgcagc gcactgagga acagttccat cagcgcactg acctcctcgg
     4081 ccgggcgctg ccagttttca ctggagaagg cgaacagcgt cagcacctca acgccggcct
     4141 cggcgcacac ttcgatcacc gcacgcaccg catcgacacc ggctttatgg ccagcaacgc
     4201 caggcagcag acgcttcttc gcccagcgat tgttaccgtc catgatgatc gccacatgcc
     4261 gtggcacggc gaacggacta ccgtctttgg tcttttccat gacgatatcc tggccgttaa
     4321 acggccatca gatccgcttc tttctgcttc acggccactt cgatttccgc gacgaacttg
     4381 tcggtcagct tctggatttc gtcggcggca cggcgctctt cgtcttcgct gatttccttc
     4441 tccttgacca aatctttcag ctggctcaag gcatcacgac ggatattgcg cacggctacg
     4501 cgaccatctt cagccgcatc acgggcctgc ttggtgaagc ccttgcgggt ttcttcggtc
     4561 agcgctggca tggaaatcag cagcagctca ccgaggttgg tcggattgaa gcccagaccg
     4621 gagctctgga tcgccttgtc gacagccgcc agcatattgc gctcgaacgc tacaacctgc
     4681 agggtgcgcg agtccttcac agtgacgtta gcgacctggt tcagcggggt gtccgatccg
     4741 tagtaaggca ccatcacacc gccgaggatg ctcgggtgcg cctgaccggt acggattcgg
     4801 ctgaaggcat ggagcagcga ttccaggctc ttctgcatgc gtacctgagc gtctttcttg
     4861 atctcattga tcatgtttat cttcctcaat cagagtgcct tcagcgccgc cgactacaac
     4921 gttcagcagg gcaccgggct tgttcatgtt gaaaacgcgc agcggcatat tatggtcgcg
     4981 gcacagacag atggccgtca gatccattac gcccagcttg cggtcgagca cctcatcgta
     5041 ggtcaggcgc tcgaacttct cggcatgcgg gtctttgaat ggatcggcag tgtacacacc
     5101 atccaccttg gtcgctttga gcaccacgtc agcatcgatt tcgatggcgc gcaggcaagc
     5161 ggccgagtcg gtagtaaaga acggattacc agtgccagcg gcgaaaatca ccacctcacc
     5221 ggttttcaga tgacgcagtg ccttgcgccg gtcgtagtga tcggtcaccc caaccatgga
     5281 gatggccgac ataacgattg ccgggatatt cgagcgctcc agcgcgtcgc gcatggccaa
     5341 ggcgttcatc acggtagcca gcatgcccat atggtcgccg gttacccgat ccatgcctgc
     5401 cgcgctcagc gccgcgccac ggaacaggtt gccaccgccg atcaccagac cgacctgcac
     5461 gccgataccg accagttggc cgacttccag cgccatgcgg tcaagcacct tgggatcgat
     5521 gccgaaatct tccgacccca tcagggcctc gccgctaagt ttgagcagaa tgcgcttgta
     5581 gcgcggttga cgaccactca tctgctgagc cattgcgagt ctctcctgcg gcgtttgaat
     5641 tctgtgcaaa ccttcggttc gcttt
//
LOCUS       DASRUM010000216         5928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_946057, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000216 DASRUM010000000
VERSION     DASRUM010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946057"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..1318
                     /gene="tig"
                     /locus_tag="VFF22_14500"
     CDS             8..1318
                     /gene="tig"
                     /locus_tag="VFF22_14500"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HZX18057.1"
                     /translation="MQVSVESTSALERRMTVGVSVERIETEVNKRLQQTARRAKVPGF
                     RPGKVPMSVIRQRYEDAARQEALGDLIQSTFYEAVVEQKLNPAGAPAVEPKSFEKGKD
                     LEYVATFEVFPEFTVAGFESIAIERLQADVADADVDNMLEILRKQNTRFEAAERAAEN
                     GDQLNIDFVGKIDGEAFAGGSAKGTQLVLGSGRMIPGFEDALVGAKAGEERVINPTFP
                     ADYQNLDLAGKTAEFTVTVNSIAAPQLPELNDDFFALFGVKEGGLEGFRAEVRKNMER
                     ELRQAIKSKVKNQVMDGLLAANPIEVPKALISNEVNRLRVQAVQQFGGNLKPEQLPAE
                     LFEEQAKRRVVLGLVVAEVVKQCELKPDDARVREMIQEMASAYQEPEQVVAWYYKNDQ
                     QMNEVRSVVLEEQVVDTVLQKANVTDKAVSYEEAVKPVEAPQAA"
     gene            1409..2044
                     /gene="clpP"
                     /locus_tag="VFF22_14505"
     CDS             1409..2044
                     /gene="clpP"
                     /locus_tag="VFF22_14505"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HZX18058.1"
                     /translation="MSRNLFMQQMPDIQAAGGLVPMVVEQTARGERAYDIYSRLLKER
                     VIFLVGPVEDYMANLICAQLLFLEAENPDKDIHLYINSPGGSVTAGMSIYDTMQFIKP
                     NVSTTCIGQACSMGAFLLTAGAEGKRYCLPNSRVMIHQPLGGFQGQASDIEIHAKEIL
                     FIRERLNMLMAKHSGRTLEEIERDTNRDNFMSAEAAREYGLIDAVIEKRPA"
     gene            2156..3436
                     /gene="clpX"
                     /locus_tag="VFF22_14510"
     CDS             2156..3436
                     /gene="clpX"
                     /locus_tag="VFF22_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003239947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HZX18059.1"
                     /translation="MTDTRNGEDNGKLLYCSFCGKSQHEVRKLIAGPSVFICDECVDL
                     CNDIIREEVQEAQAESSAHKLPAPKEISTILDQYVIGQERAKKVLAVAVYNHYKRLNQ
                     RDKKDDVELGKSNILLIGPTGSGKTLLAETLARLLNVPFTIADATTLTEAGYVGEDVE
                     NIIQKLLQKCDYDVEKAQMGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEG
                     TVASVPPQGGRKHPQQEFLQVDTRNILFICGGAFSGLEKVIQGRSTKGGIGFNAEVRS
                     KEEGKKIGESLRAVEPDDLVKFGLIPEFVGRLPVIATLDELDEAALVQILTEPKNALT
                     KQYAKLFEMEGVELEFRPDALKSVAHKALERKTGARGLRSILEGVLLDTMYEIPSQSD
                     VSKVVIDESVIDGTSKPLLIYENNELPAKAAPDA"
     gene            3586..>5928
                     /gene="lon"
                     /locus_tag="VFF22_14515"
     CDS             3586..>5928
                     /gene="lon"
                     /locus_tag="VFF22_14515"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZX18060.1"
                     /translation="MKTTIELPLLPLRDVVVYPHMVIPLFVGREKSIEALEAAMAGEK
                     QILLLAQRNPADDDPDEAGLYRVGTVATVLQLLKLPDGTVKVLVEGEQRGEIERFIEA
                     DGHCRAEVQLIDEIDAAERESEVFTRSLLSQFEQYVQLGKKVPAEVLASLNGIDEPSR
                     LVDTMAAHMVLKIEQKQEILEITDLAARVEHVLAQLDAEIDLLQVEKRIRGRVKKQME
                     RSQREYYLNEQMKAIQKELGDSEEGHNELDELKKRIENAGLTAEAHTKANAELNKLKQ
                     MSPMSAEATVVRSYIDWLVNVPWKAQSKVRLDLARAEAILDADHYGLDEVKERILEYL
                     AVQKRVKKIKGPVLCLVGPPGVGKTSLAESIASATNRKFVRMALGGVRDEAEIRGHRR
                     TYIGSMPGRLIQKMTKVGVRNPLFLLDEIDKMGQDMRGDPASALLEVLDPEQNHNFND
                     HYLEVDYDLSDVMFLCTANSMNIPGPLLDRMEVIRLPGYTEAEKINIAIKYLAPKQVQ
                     ANGLKKAELVFEDAAIRDIIRYYTREAGVRGLERQIAKVCRKVVKEHSAEKRFQVSVT
                     ADSLEHFLGVRKHRYGLAEQQDQIGQVTGLAWTQVGGELLTIEAVVVPGKGQLIKTGS
                     LGDVMLESITAAQTVVRSRAKSLGVPVDFYEKQDIHIHMPEGATPKDGPSAGVGMCTA
                     LVSALTQIPVRADVAMTGEITLRGQVLAIGGLKEKLLAAHRGGIKTVIIPEENVRDLK
                     EIPENIKQDLQIKPVKWIDEVLQIALQYAPEPLPDAAPMVA"
BASE COUNT         1339 a         1571 c         1761 g         1257 t
ORIGIN      
        1 attatccatg caagtttctg ttgaaagtac ctctgctctc gagcgccgta tgaccgttgg
       61 cgtctctgtt gagcgcatcg agactgaagt caacaagcgt ctgcagcaga ctgcccgtcg
      121 tgccaaggtc cctggtttcc gtcctggcaa agtgccaatg agcgtgatcc gtcagcgtta
      181 tgaagatgct gcgcgtcagg aagctctggg cgatctgatc caatccactt tctacgaagc
      241 cgttgttgag cagaagctca atccagctgg cgctcctgcc gttgagccga agtcgttcga
      301 gaaaggcaag gatctggaat acgttgccac cttcgaagtg ttccctgaat tcaccgttgc
      361 tggcttcgaa tccatcgcta tcgaacgcct gcaggctgac gtagctgacg ctgacgtcga
      421 caacatgctg gaaatcctgc gcaagcagaa cacccgtttc gaagccgccg agcgcgctgc
      481 cgagaatggc gaccagctga acatcgactt cgttggcaag atcgacggcg aagccttcgc
      541 tggcggttcg gccaagggta ctcagctggt tctcggctcc ggccgcatga ttcctggctt
      601 tgaagatgca ctggtgggcg ccaaggctgg cgaagagcgc gtaatcaacc caactttccc
      661 ggctgattat cagaacctcg atctggcggg taaaactgcc gagttcaccg tcaccgtcaa
      721 cagcatcgcg gctcctcagc tgcctgagct gaatgatgac ttcttcgccc tgttcggcgt
      781 gaaagaaggc ggtctggaag gcttccgcgc tgaagtgcgc aagaacatgg agcgcgagct
      841 gcgccaggcg atcaagtcca aggttaaaaa ccaagtgatg gacggcctgc tggctgccaa
      901 cccgatcgaa gtgccgaaag cactgatcag caacgaagtg aaccgtctgc gcgtgcaggc
      961 cgttcagcag ttcggtggca acctcaagcc tgagcagctg ccagctgagc tgtttgaaga
     1021 gcaagccaag cgtcgcgttg tgctgggtct ggtcgtagct gaagtggtca agcagtgtga
     1081 gctgaagccg gacgacgccc gcgttcgcga aatgattcag gaaatggctt cggcttacca
     1141 agagcctgag caggttgtgg cctggtacta caaaaacgac cagcaaatga acgaagtacg
     1201 ttcggttgta ctggaagagc aagttgtaga tactgttttg cagaaggcca acgtgaccga
     1261 taaagcggtc tcgtacgaag aagctgtcaa gccggtagaa gcaccacaag ccgcctgatt
     1321 tctttacctc gttcgaacac gccataagcc agcctccgcg ctggcttatg cgtatttgtg
     1381 acatgactat ttagggagcg agtgccgcat gtcccgcaat ctttttatgc agcaaatgcc
     1441 tgatatccaa gccgctggcg gcctggtacc gatggtcgtc gagcagacgg ctcgcggtga
     1501 gcgcgcttac gatatctatt cgcgcctgct caaggagcgt gtgatctttc ttgtcggtcc
     1561 tgtagaggac tacatggcca acctgatctg tgcgcaactg ttgttccttg aagcggaaaa
     1621 tccggataag gacattcacc tttacatcaa ctctccaggt ggttcggtga cggcgggtat
     1681 gtcgatctac gacaccatgc agttcatcaa gcctaacgtg tccaccacct gtattggtca
     1741 ggcgtgcagc atgggtgcat tcctgctgac cgctggcgct gagggcaagc gttactgtct
     1801 gccgaactcg cgcgtgatga ttcaccagcc gctgggcggt ttccaaggcc aggcgtcgga
     1861 cattgaaatt cacgccaagg aaatcctctt tatccgtgag cgtctcaaca tgctgatggc
     1921 caagcacagc gggcgcaccc tggaagaaat cgagcgcgat accaatcgtg ataacttcat
     1981 gagcgccgaa gccgctcgtg aatacgggtt gatcgacgca gtgattgaga aacgccccgc
     2041 ttaatataag cagctcaaaa tagggcaggt cggcgtgtcc ggccgccggc gggcttgaaa
     2101 atgcccgcat ttgccctcat cttgtgtttc aagcctaccg gattggattg aacgaatgac
     2161 tgacacccgc aacggcgagg acaacggcaa gctgctttat tgctccttct gcggcaaaag
     2221 ccagcatgaa gtgcgcaaat tgattgccgg cccctcggtc tttatctgcg acgagtgcgt
     2281 cgacctgtgc aacgacatca tccgcgagga ggtgcaggaa gcccaggccg agagcagcgc
     2341 gcataaattg cctgcaccca aggaaatcag cactatcctc gatcagtatg tgatcggtca
     2401 ggagcgtgcg aagaaggttc tggcggtagc ggtatacaac cactacaagc gcctcaacca
     2461 gcgcgacaag aaagatgatg tcgagctggg caaaagcaat atcctgctga ttggtccgac
     2521 tggctcgggt aaaaccctgt tggctgaaac cctggcacgc ttgcttaacg taccgttcac
     2581 catcgccgac gccaccaccc tgaccgaagc cggttatgtc ggtgaggatg tcgagaacat
     2641 cattcagaag ctgctgcaga agtgcgatta cgatgtggaa aaagcccaga tgggcatcgt
     2701 ctacatcgac gaaatcgaca agatttcgcg caagtcggat aacccgtcga ttacccgtga
     2761 tgtctcgggc gagggcgtgc agcaggcatt gctcaagctg atcgagggta cggttgcttc
     2821 ggtgccgccg cagggtggtc gcaagcatcc gcagcaggag ttcctgcagg ttgatacgcg
     2881 caatatcttg tttatctgcg gtggtgcgtt ctctggtctg gaaaaagtca tccagggccg
     2941 ttcgaccaag ggcggaatcg gtttcaatgc cgaggttcgt agcaaggaag agggcaaaaa
     3001 aatcggcgag tcactgcgcg ccgttgagcc tgatgacttg gtcaagtttg gtctgattcc
     3061 tgagttcgtg ggccgcttgc cggttatcgc cacgctggat gaattggacg aagcggctct
     3121 ggtacagatt ctgaccgaac cgaagaacgc gttgaccaag caatacgcca agctgttcga
     3181 gatggaaggg gttgagctgg aattccgtcc ggatgcgctg aagtcggttg cgcacaaggc
     3241 acttgaacgt aagaccggtg cccgcggcct gcgttcgatt ctcgaaggtg tcctgctcga
     3301 taccatgtac gagatcccgt cgcagtccga tgtcagcaag gtggttatcg atgaaagcgt
     3361 tatcgatggc acctccaagc cgttgctcat ttatgagaac aacgagctgc cagccaaggc
     3421 tgcgcctgac gcgtaacagc gttgtaacat gttgaaagca aggggcctgc gggccccttt
     3481 gcttttgctg tgtcgacgct tgttttttgt tggggctgcc cccatcttag ggttaagggt
     3541 tatcccatcc gttcacggcc atatggccgc cgtagagccg aaatcatgaa gacaaccatc
     3601 gaattgcctc tcctgccgtt gcgcgatgtt gtggtctatc cgcacatggt cattccgtta
     3661 ttcgtcgggc gtgagaaatc cattgaagcc cttgaggcag cgatggcggg cgagaagcag
     3721 atactgctcc ttgcccaaag gaatcctgcc gacgacgatc cggatgaagc cggcctttac
     3781 cgtgtaggta ccgtcgccac tgtgttgcag ctgctgaaat tgccagacgg caccgtcaag
     3841 gtgttggtcg agggcgagca gcgcggcgag attgagcgct ttatcgaggc cgacggtcat
     3901 tgccgggcag aagtgcaact gatcgacgag attgatgctg ccgagcgtga gtccgaagtc
     3961 ttcacccgca gcttgcttag ccagttcgag cagtacgtgc agcttggtaa gaaggtccct
     4021 gccgaagtgc tggcatcgct caatggtatc gatgagccga gccggctggt cgacaccatg
     4081 gctgcgcata tggtgctgaa gattgagcag aagcaggaaa ttctggaaat caccgatctg
     4141 gctgcgcggg ttgagcatgt cctggcgcag ctcgatgcgg aaatcgacct actgcaggtc
     4201 gagaagcgca tccgtggccg ggtcaagaag cagatggaac gcagccagcg cgagtactac
     4261 ctgaatgagc agatgaaggc cattcagaag gagctcggtg acagcgaaga aggtcataac
     4321 gaactcgatg agctgaaaaa gcgcatcgag aacgccggcc tgaccgccga ggcgcatacc
     4381 aaggccaatg ccgagctgaa caagctcaag caaatgtcgc cgatgtcggc tgaagccacc
     4441 gtggtgcgtt cttacatcga ttggctggtc aatgtcccat ggaaagcgca gagcaaggtg
     4501 cgcctggatc tggcgcgtgc cgaggcgatc cttgatgccg atcactacgg tctggatgag
     4561 gtcaaggaac gcatccttga atacctcgct gtgcaaaagc gtgtgaagaa aatcaaaggc
     4621 ccggtgctct gtctggtcgg tccgcctggc gtaggtaaga cttcgctggc cgaatcgatt
     4681 gccagtgcca ccaaccgcaa gtttgtgcgg atggcgctcg gcggcgtgcg tgatgaggcc
     4741 gagattcgtg gtcatcgccg cacctatatc ggctctatgc cggggcgtct gattcagaag
     4801 atgaccaagg ttggagtgcg taacccgcta ttcctgctcg atgaaatcga caagatgggc
     4861 caggacatgc gtggcgatcc tgcctcggca ttgcttgagg tactggaccc agagcagaac
     4921 cacaatttca acgaccacta tctggaggtc gactacgacc tgtccgatgt gatgttcctt
     4981 tgcacggcca actcgatgaa cattccgggt ccgttgctgg accgcatgga agttattcgt
     5041 ctgccgggtt acaccgaggc cgagaagatc aatatcgcga tcaaatacct ggcgcccaag
     5101 caggttcaag ccaatggttt gaagaaggct gagctggtat ttgaagacgc ggctatccgc
     5161 gacatcattc gctactacac ccgcgaggct ggtgtgcgtg gtctggagcg gcagattgcc
     5221 aaggtgtgcc gcaaggtggt taaggaacac tccgcagaga agcgcttcca ggtctccgtt
     5281 acggctgatt cgctcgagca tttcctcggt gtgcgcaagc atcgctacgg tttggccgag
     5341 cagcaggatc aaattggcca ggtcacaggc cttgcctgga cccaggtcgg cggcgagttg
     5401 ctcaccatcg aagcggtcgt ggtgcctggc aaaggccaac tgatcaaaac cggctcgctc
     5461 ggtgatgtga tgctggaatc catcaccgct gcacagactg ttgtgcgtag tcgggccaag
     5521 agcctgggcg tgcctgtgga cttctatgag aagcaggaca tccatatcca tatgccggaa
     5581 ggggcgacgc ctaaagacgg ccccagcgca ggcgttggca tgtgtacggc gctggtttca
     5641 gccctgacgc agatccctgt gcgtgcggac gtggcaatga ccggtgaaat caccctgcgt
     5701 ggtcaggtat tggccattgg tggtttgaaa gaaaaactgc tggccgctca ccgtggtgga
     5761 atcaaaaccg tgatcattcc tgaagaaaat gtgcgcgatt tgaaagagat tccggagaat
     5821 attaagcaag acctgcagat taaaccggtt aaatggattg acgaggtcct gcaaattgcg
     5881 ctgcaatacg ccccggagcc cttgcctgat gcggctccca tggttgca
//
LOCUS       DASRUM010000217        46652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2436591, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000217 DASRUM010000000
VERSION     DASRUM010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 46652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46652
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2436591"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFF22_14520"
     CDS             <1..330
                     /locus_tag="VFF22_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016487172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18061.1"
                     /translation="PPVDEVPQEPREPLDDLGLTGAATQTDSAHEDNVTLDDLSPDTL
                     FDESGARSPIEPGGDGPADEELRTVDESEIGGGIGLDEAELGHSAPLDGKPWTDEVSP
                     TDKERKR"
     gene            complement(374..748)
                     /locus_tag="VFF22_14525"
     CDS             complement(374..748)
                     /locus_tag="VFF22_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="termination factor Rho"
                     /protein_id="HZX18062.1"
                     /translation="MPRGSKDSYSEKQKRKAEHIEDSYKAKGVPEEEAEARAWATVNK
                     QSGGGEKSGSGRTTSATAKQSARKSSARRAVATRKGTPRPGEKLEAMNKTELLDLARK
                     RNLAGRSSMDKSELLEALRKNS"
     gene            922..1416
                     /locus_tag="VFF22_14530"
     CDS             922..1416
                     /locus_tag="VFF22_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HZX18063.1"
                     /translation="MDVIERILATLVLEFSDLQQVEHLTRTTLRLFLATLLGGLLGYE
                     REVMGKAAGIRTHMLVCLGAAIFVMPIEQAGADHDAMSRVIQGVAAGIGFLCAGTILK
                     GQNISDVKGLTTAAGLWTSAAIGVAVGLGREATAVLSTVLVLMVLHIMPLIVDPNPNG
                     KNKE"
     gene            1557..3542
                     /locus_tag="VFF22_14535"
     CDS             1557..3542
                     /locus_tag="VFF22_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HZX18064.1"
                     /translation="MPSPASANEPIFRALQQPRLAIENIQPALEAGRFAVKCLCGRPV
                     QVSAIIFADGHDQLAADVLWRANDQAGWQRIAMQALGNDRWSASLLIETIGAAEFVIE
                     AWHDVYASFRHELGKKFAAGAPVSLELQEGAELVRRICAGAPASLKDQLTELVNRMEA
                     AQSIEERVAILLDPAISELMRLAEYRPHLLRSAVYPLDVERRLAEFASWYELFPRSES
                     AEPGQHGTFADVHRRIPDIARMGFDVLYFPPIHPIGSTHRKGPNNSLKAGPDDPGSPY
                     AIGSQDGGHEAVHPQLGSLEDFRELVRVAHQHGLEVALDFAVQCSPDHPWLREHPGWF
                     SWRADGSIRHAENPPKKYEDIVNVEFYAPEAIPSLWLALRDVVMGWVELGVTLFRVDN
                     PHTKPMPFWEWLIADVRGRNPQVIFLAEAFTRPAMMARLGKLGFSQSYTYFTWRNSKT
                     ELQDYFTELNQPPWRDCYRPNFFVNTPDINPYFLQRSGRPGFLIRAALATMGSGLWGM
                     YAGFELCEAAPVPGKEEYLDSEKYQLRSRDYQAPGNISAQIARLNQIRRDNPALQTHL
                     GFQAYTVWNEQILYFGKRTADRSNFILVAISLDPVNTQEANFELPLWELGLADDADLE
                     GEDLMNGHRWTWHGKVQWMRIDPWHLPFGIWRIHSAR"
     gene            3562..6867
                     /gene="treS"
                     /locus_tag="VFF22_14540"
     CDS             3562..6867
                     /gene="treS"
                     /locus_tag="VFF22_14540"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009876377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HZX18065.1"
                     /translation="MAKRSDANAFLNDPQWYKDAVIYQVHVKSFFDANNDGIGDFQGL
                     IEKLDYIAELGVNTLWLLPFYPSPRRDDGYDIAKYRGVHPDYGNLADARRFIAEAHRR
                     GLRVITELVINHTSDQHPWFQRARHARKGSKARNYYVWSDSDEKYDGTRIIFIDSEKS
                     NWTWDAVAGQYFWHRFYSHQPDLNFDNPVVLNAVLGVMRFWLDMGVDGLRLDAIPYLI
                     ERDGTNNENLPETHAVLKRIRAELDSRYPDRLLLAEANQWPEDTRPYFGDDDECHMAF
                     HFPLMPRMYMAIAQEDRFPITDILRQTPEIPPNCQWAIFLRNHDELTLEMVTDDERDY
                     LWNYYAADSRARINLGIRRRLAPLVSKDRRRIELLNSLLLSMPGTPTLYYGDEIGMGD
                     NIYLGDRDGVRTPMQWSVDRNGGFSRADPASLVLPPIMDSLYGYQTINVEAQARDQHS
                     LLNWMRRMLNIRNQYKAFGRGALKMLAPSNRRILSYLRTYTNDEGHEENILCVANLSS
                     AAQAVELELADYRDRVPVEMLGGAPFPPIGQLTYLLTLPPYGFYWFYLADASQMPSWH
                     AKPVERMPELQTLVLSRSLSEVMNDRPRRTLEQESLPLYLPKRRWFAGGQRPGGEVRL
                     LYVIPLAEGSNAPLLAEVAVSGEQGVEHYRLPLAAVAERAIGNDLPQQLALARLRRGR
                     HVELLTDAFSLPAFSLQVLRLLRSNTVLRSDAGEIQFRAEPALLEGAQVDEAQVTLLS
                     TEQSNSSALIADQLMLKLLRRGFPGIHPEVEMGRFLTHQGYANVAPFLGEVVRADEDG
                     VPHTLMVAQEYLNNQGDAWIWTLNTLDRAIRDELAGGTRDPEIDALRELEIFSAVLGK
                     RVGEMHAVLARPVENPDFGYQLSDEQDSAAWATAIGEQLREALDALQQNREHVPAALV
                     EQLDWLLAQKNQLLHALDALATQTVGGLRIRVHGDLHLGQVLVVQGDAYIIDFEGEPN
                     RPLAERRRRHSPTKDVAGMLRSFDYAAAVAQRNAGGVESTAQSQALVHEISARYRNAS
                     RTAFMDAYRLAAGGLPHEWHARDGEGAAVDLFCLEKAAYEVVYESGHRPDWIDVPLQG
                     LLDLAHQLTGAHR"
     gene            6864..9023
                     /gene="glgB"
                     /locus_tag="VFF22_14545"
     CDS             6864..9023
                     /gene="glgB"
                     /locus_tag="VFF22_14545"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HZX18066.1"
                     /translation="MNQDRVERLLRAEDGDPFSFLGPHPQEGGTLVRVWMPNAEGVEL
                     LTRDGQSLGAMQRTDAQGLFELSISQAQSYCLRIQWPDGQQETEDPYAFGPLLGELDL
                     YLFAEGNHRELWRCLGAQVETRDGISGVRFAVWAPNAQRVSVVGNFNSWDGRRHPMRL
                     RYPAGVWELFIPRLQPGECYKYEILSAGRVLPLKADPLAQATEVPPATGSRVPQPAAF
                     AWQDQQWMEHRQQAQGRHTALSIYELHATSWQWHDERPPSWDELADRLIPYVQDLGFS
                     HIELMPIMEHPFGGSWGYQPLSMFAPTSRLGDAQGFAAFVDRCHRAGIGVILDWVPGH
                     FPNDAHGLAEFDGTALYEYAHPFEGFHPDWNTCIYNLGRTEVHGFMLASALYWLREYH
                     IDGLRVDAVASMLYRDYSREEGEWIPNRHGGRENLEAIEFLQHLNEVISSEVPGALVI
                     AEESTAWPGVSRPVQEGGLGFSHKWNMGWMNDSLSYVQKDPVYRMHHHHQITFGLHYA
                     FSERYILPISHDEVVHGKRSLLGRMPGDRWQQFANLRLYFAFMWTHPGKKLLFMGCEF
                     GQWREWNHDQQLDWYLLQYPEHAALQSLLRELNHLYRHEPALHGQDECEAGFQWLIGD
                     DARNSVFAWVRHAAGHPPLLVVHNFTPQVHAAYRVGVSRAGAWEVLLNTDELRWSGSG
                     AGSKGELESEAVTAHGERDSLQLELPPLGTLILRPRQ"
     gene            complement(9078..9491)
                     /locus_tag="VFF22_14550"
     CDS             complement(9078..9491)
                     /locus_tag="VFF22_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZX18067.1"
                     /translation="MKISEIMTRNVQTTSPERSLRDVAALMADIDSGAVLVNEGDRLV
                     GMVTDRDIAIRGVAAGLTSDTPVRKVMSGEVRYCFDDEEVEHVAKNMADIQMRRLPVL
                     NREKRLVGVVSLGNIASCRNERASATVLQGVAQAH"
     gene            complement(9592..10362)
                     /locus_tag="VFF22_14555"
     CDS             complement(9592..10362)
                     /locus_tag="VFF22_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014591709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZX18068.1"
                     /translation="MTAQSEAIEIIVENESIAGTVLSPETKVPGVLFVHGWGGSQQRD
                     LARAKGIAGLGCVCLTFDLRGHERTIAAKECVSREHNLTDIVAAYDRLANHPAVDPCC
                     IAVIGSSYGAYLSTHLSARRAVRWLALRVPALYWDEDWHMPKRQLDVAKLAQYRRHLW
                     KAQDNLALAACANFSGDVLLVESEHDDFVPHTTLMSYRAAFEKAHSLTHRIIDGADHA
                     LSKDSDQQAYTRILTNWISEMVVGARVGDYPYHSVRYS"
     gene            complement(10371..10586)
                     /locus_tag="VFF22_14560"
     CDS             complement(10371..10586)
                     /locus_tag="VFF22_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18069.1"
                     /translation="MSKPTHLISYLLHDHPHTVEIAAESAELTLEQARLRLNSIHTFE
                     WPNEFSDVQVTPIAQPGGTTSSPRAQG"
     gene            complement(10688..11668)
                     /locus_tag="VFF22_14565"
     CDS             complement(10688..11668)
                     /locus_tag="VFF22_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010954144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HZX18070.1"
                     /translation="MSSHQQSAWVVWGKRMLSVFFLLLIPTLLYLLAKNLDWDEVWAA
                     LSDYSAQTLLMGILLVFCSYLVFSLYDLLGRYYTRHKLPARQVIPVAMVCYAFNLNFT
                     TWVGGVALRYRLYTRLGLKASTVTRIISLGLLTNWTGYMALAGVIFSLRLIQLPEHWE
                     LGTAGLQLIGFLLLGLVAIYIAFCAFAKQRVWVWREHSITLPSVRLAVLQLCLGASNW
                     ALMASLIYLLLPANAFYPSVLGVLLISCVAGAITHIPAGLGVLEAVFLALLQHQFSQG
                     SLVAALLGYRALYFLLPLLIASVAYLILEKRAKHLRQQHESSNRPASCKP"
     gene            complement(11665..12891)
                     /gene="clsB"
                     /locus_tag="VFF22_14570"
     CDS             complement(11665..12891)
                     /gene="clsB"
                     /locus_tag="VFF22_14570"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HZX18071.1"
                     /translation="MSGVWRDGNQIQLLINGEEFYPRVFERIRAAREEVLLETFIIME
                     DKVGLALQQVLIETARRGVRVVLAVDGYGTADLGSEYVAAMAREGIQVHVFDPSPRRL
                     GMRTNLFRRLHRKIVVIDGERAFVGGINYSADHLGDFGEMAKQDYAVEVSGPIVRDLH
                     SSTMRLLRSALIAPSPTQPLAKPVGDVRIMLLERDNERHTTDIEEQYLSAFRAAKERL
                     IVANAYFFPSYKVLRELRNAARRGVKVTLILQGQPDMAWAQGFSRLLYNYLLRDGVDI
                     YEYCQRPLHGKVALVDHEWSTVGSSNLDPLSLALNLEANLFISDRAFNQQLYDHLQQL
                     SAEHCTRIRLERILRGYWWRAPLVFLCFHFIRLFPAIPGLLPAHRKNLKPLTPTPEVQ
                     TQTQDKPCYCKEEKTG"
     gene            complement(12888..13676)
                     /locus_tag="VFF22_14575"
     CDS             complement(12888..13676)
                     /locus_tag="VFF22_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012052310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZX18072.1"
                     /translation="MSTTIPQPEMVIDRITTVQRLQVMTINTHKGFTALNRRFILPEL
                     RSAVQATGADLVFLQEVLGSHALHAKRFRDWPDIPQYEFLADSMWPQFAYGRNAVYPN
                     GHHGNALLSKFSIVEHNNLDVTVKGNEQRGLLHCQLDVPGHAEVHAICVHLGLRDSHR
                     RKQISLLLDLLASLPATAPVIVAGDFNDWSLRADALLAEQSLVEAFGATYGAPAKSFP
                     ARFPLLCLDRIYVRNATAHTPVVLSTRPWSHLSDHAPLTAEIHL"
     gene            complement(13763..15040)
                     /locus_tag="VFF22_14580"
     CDS             complement(13763..15040)
                     /locus_tag="VFF22_14580"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HZX18073.1"
                     /translation="MKAVVFHDIGDIRLDDVPEPTIQAPTDAIIRLTASAICGTDLHF
                     VRGTVSGMVKGTILGHEGVGVVEALGSDVRNLSIGDRVVVPSTIACGNCTYCRAGYYA
                     QCDEANPNGKEAGTAFYGGPAATGAFHGLQAELARIPYANVGLVKLPSEISDDQAILL
                     SDIFPTGYFGAELAEIGSGDTVAVFGCGPVGQFAIASAKLLGASRVFAIDRLEDRLHM
                     ARQQGAEVINFDQEDPVQTLRRLTNGIGVDRAIDAVGVDAECPAHCHHDSEQAQTQSS
                     NAWQPPGNAPSQALQWAVEGLAKAGTLGIIGVYPQQAEHFPIGMAMNKNLTINMGNCN
                     HRRYIPRLIEMIQAGRIDPSKILTQVKPMNDAIEAFKAFDRRESGWIKVELRPSKRTS
                     SGSEEQVRDAALDRVIAEASPYHESRSEKPGQL"
     gene            complement(15197..17326)
                     /gene="glgX"
                     /locus_tag="VFF22_14585"
     CDS             complement(15197..17326)
                     /gene="glgX"
                     /locus_tag="VFF22_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017131219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HZX18074.1"
                     /translation="MSTASNRQHSRVTEGNPFPLGATWDGLGVNFALFSAHATRVELC
                     LFDDQGNEEVERIELPEYTDEIWHGYLPDARPGQIYGYRVYGPYEPEAGHRFNHHKLL
                     LDPYAKQLVGQLQWSEALFGYTIGSPDGDLSFDERDSAAFVPKCKVIDPAFTWGEQAP
                     IRVPWDNMLIYEAHLRGISMRHPAVPEAHRGTCAALTNPELLRHIRALGVSSLELLPV
                     HAFVNDQHLLEKGMNNYWGYNSIGFFAPHPAYLASGKVSEFKEMVAHLHAAGLELILD
                     VVYNHTAEGNERGPTLCMRGIDNSSYYRLLPEDRRYYINDSGTGNTLDLSHPCVLQMV
                     TDSLRYWASEMRVDGFRFDLATILGRHAHGFDERHGFLVACRQDPVLSKVKLIAEPWD
                     CGPGGYQVGGFPPGWAEWNDKFRDNVRAFWKGDEGELAELASRLTASGDHYNQRGRRP
                     FASVNFITAHDGFTLRDVVSYDHKHNEANDENNQDGTDHNRSWNHGCEGATDDPAIRQ
                     LRLQQMRNLLATLFFAQGTPMLLAGDEFGRTQHGNNNAYCQDNELSWVDWNIDDEGEA
                     LIAFCQRLIALRRSHPILRRRRFLVGHYNEELGVKDVTWLAPDGEEMTEAHWHDNEAR
                     CMGMLMDGRAQPTGVKRSGADATLLLLLNAYHDSRSFVLPEVAGGKRWSCLINTHQPV
                     IEGAPQPLQYATLKLAGRSLQLLELQR"
     gene            complement(17332..17604)
                     /locus_tag="VFF22_14590"
     CDS             complement(17332..17604)
                     /locus_tag="VFF22_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HZX18075.1"
                     /translation="MSIDEQRIREFAFQIWESEGKPIGQEERHWQMARKLAEAENTAE
                     VLAPAPAKPRRISKPKTLPLSEPEQPALLKKPRAPRSSAPKTPKPE"
     gene            complement(17594..20395)
                     /locus_tag="VFF22_14595"
     CDS             complement(17594..20395)
                     /locus_tag="VFF22_14595"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003289709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HZX18076.1"
                     /translation="MTELLSTLRLQFHHGFTLYDALPLIPYFERLGVSHLYASPLLTA
                     RPGSMHGYDVIDPSKINPELGGEAALVRLADALHARGMGLILDIVSNHMAVGGDANTW
                     WLDVLEWGRHSPYATFFDIQWNSHDPLLEGQLLLPFLRSDYGEALREATLTLRFDEQH
                     GRFYVEHFEHRLPITPPSYNDILGATEEPALAQLGQRFAALGQGAGTRELAQALCAEL
                     AEWAQNTAQSTHLEALLERYRRVDKPSQQRLHRLLERQHYRVASWRTAADDINWRRFF
                     DINELAGLRVERPPVFEKTHAKIFELIERGLIDGLRIDHIDGLANPRTYCKRLRRRID
                     SLRTAQAPRPLPIYVEKILGHGENLPQDWAVDGTTGYEFMNQVSLLQHDPAGEQPLRE
                     LWQTLSGRPALFQEEVLEARRLVLTGSLAGDVEEVAQRLLQVARNDIDTRDLTLGSIR
                     RALLELIVHFPVYRTYAQACGRSEQDRKFFRQALDGAYSTLAEADWPLLEYLDRWLGG
                     EPLRELPPGRPRTLRAQVLTRFQQLTSPIAAKAVEDTAFYRSAVLLSRNDVGFDAEHF
                     SRPAAHFHQCCLERAKHYPRNLLSTATHDHKRGEDSRTRLAVLSERAPWFVERVRQWS
                     EHAAALRSAATPNAPSRGEELMLYQALLASWPLDLQADDQPGLQAYLQRMTAWQEKAL
                     REAKLNSAWSAPNSEHEAACADFLTRLLTAPEGRELLNALADTVNRIAPAAALNSLVQ
                     CLLRLTTPGVPDLYQGCEYWDFSLVDPDNRRPVNFAARERSLEALQNPATLLAEWRDG
                     RIKQWLIQRVLHLRQQYPALFSRGDYRAVTVAGTHAEHVLAFTRSHGQEHLLVVVPRL
                     TAALLGNDQVPHIPAKHWGDTRLMLPAEFSGLTLLGSLSPCQTVCAQVLPVAQVLTDF
                     SVNVLCNIPAATGAPHHEH"
     gene            complement(20388..22448)
                     /gene="malQ"
                     /locus_tag="VFF22_14600"
     CDS             complement(20388..22448)
                     /gene="malQ"
                     /locus_tag="VFF22_14600"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HZX18077.1"
                     /translation="MSDTQLAELARAANLCIDWTDAYGRPHSVSPQVQRSLLEALGYP
                     AQSPEQIRASLSALAQQRDEHAADALLILNQGQPFSLNNYPAHTPYQLTDECGHVRSG
                     RLDHEAALPAIDRPGYFQLDIADNHYRLAVTPAACLSAEQLCGKQAVWGLTAQLYGLR
                     RPGDGGLGDTQAVAELARHAANQGADALALSPVHALFSADYEHYSPYSPSSRLFFNAL
                     HATPANILGRERVQRAVLACGVQAELTHLEGLQLIDWPMVAEVRQRLLRQLYKDFKQT
                     PGELLSSFQAFCAAGGSALQQHCRFEAIYEHRKQNGESTDWRTWPAGLRDAQQAEVSR
                     FAAEYGECVEYHAFAQWLIAHGLEGAQASALGAGMKIGLIADLAVGADCAGSQAWSRQ
                     GEFLPQVTVGAPPDILNRNGQNWGVSAFSPAGLRQHGFRAYLEMLQANLAHAGGIRID
                     HVMGLQRLWVIPQGADPQDGAYLQYPLNDLLRLLALESHRHRALVIGEDLGTVPHGLR
                     ETLAARNILGMRVLLFEQHEGRFIAPEKWPRNALATTSTHDLPSIKGWFAGHDIDWRV
                     RAGHSQAEQRSSDYSERERERSALVDALAESEQLQGDRDDVEACLQASIGYIGSTPAV
                     LTLLPLEDACGAEQQPNLPGPGSEHPNWRRRYALPVREMLEQPLARQRLERLAAARRE
                     IADD"
     gene            complement(22445..24199)
                     /gene="treZ"
                     /locus_tag="VFF22_14605"
     CDS             complement(22445..24199)
                     /gene="treZ"
                     /locus_tag="VFF22_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HZX18078.1"
                     /translation="MYSHGAQRLDDAQTRFALWAPSCNRVAVELQGQLPQPMHAEADG
                     WFRLDLVCQPDASYRFVINEHLRVPDPASRQQAGDVHGFSRLVDHSAYPWRHDDWNGR
                     TWVETVIYELHVGLFGGFQQVEEHLAHLAELGVTAVQIMPLGAFPGQRNWGYDGVLPF
                     APASCYGTPVQLKHLIDRAHGLGLMVFIDVVYNHFGPEGNYLGLYANSFFRTDRMTPW
                     GAAIDFRRPQVRDFFCENALMWLLDYRCDGLRLDAVHAIDDDEFLLELASRVRSAVPQ
                     PHHVHLVLENENNSASLLGNGFNAQWNDDGHNTLHVLLTGEQESYYADYAASPTHNLA
                     TFLEQGFVYQGQMNRHALPRGEPSGHLPPHAFVLFLQNHDQVGNRAFGERLINISDSD
                     ALKAATVLLLLCPMVPLLFMGEEWGSRRPFLFFSDYRDDLAQAVRDGRRNEFAEFSQF
                     ANPHTRQQIPDPNAPSTFVRSQPDFALAQRPRQQEWLAFYKDLLHVRRTRILPGLEQA
                     SSLGTQVIGDGAVLASWRIGGEKILRIAVNLGNQAVRLASAPPLAERLYAHRANDDEY
                     RQGLLPTSSALVTLELLT"
     gene            complement(24199..25731)
                     /gene="glgA"
                     /locus_tag="VFF22_14610"
     CDS             complement(24199..25731)
                     /gene="glgA"
                     /locus_tag="VFF22_14610"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HZX18079.1"
                     /translation="MGNVAAIAPPRSHSIFPASVPPSLHLESLQPQRKRILFVTSEFA
                     DLIKVGGLGDVSAALPRALSAQHDIRVLIPGYRQVLESGRALRIVGQVEGLAALPACK
                     IGRLDLADGLIVYVLLHPALFDRAGSPYGDQNGRDWPDNHIRFASLGLAAAEIARGDA
                     GIHWRPELVHANDWPAALAPAYMTWQQVKTPTLLTIHNLAYQGLCDMQCSAELAIPAH
                     ACGIEGMEFHGQLSFLKAGIFYASHVTTVSRTYAQEITTSGFGCGLDGMLRAKVKAGR
                     LSGIINGIDESWEPANDPHLVQGFSADQWQGKQANTAYVEQMFGLQPGGPLFAVVSRL
                     VQQKGIDLTLRIADQIVNNGGRLAVIGRGEAELETAMEELAKRYPGRVGVHIGFNETD
                     ARRIFAGSDFLLMPSRFEPCGLSQMYAQRFASLPIARRTGGLADTIEDGVTGFLFNEA
                     SVESYQAAVQRAFNIYQRPELLNAMRCRAMNAPLFWQQSVTPYDRLYQHLLSEAHAAV
                     SL"
     gene            complement(26053..26577)
                     /locus_tag="VFF22_14615"
     CDS             complement(26053..26577)
                     /locus_tag="VFF22_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HZX18080.1"
                     /translation="MQPFKYLSLLAIAAFAFCLANAAHAADQVDPISAEDFVEEASAK
                     GIAEIETARLALEKASSQAIKDFAQTMIDDHSKANLTLNQLASQKELNVSDDAELINQ
                     AKALILKLRDEESFDKAYMNNQVMAHKQTIELFRRGSESDDKEIAAFAQETLPKLQKH
                     LQMAEEINAQTPGD"
     gene            26789..28930
                     /gene="katE"
                     /locus_tag="VFF22_14620"
     CDS             26789..28930
                     /gene="katE"
                     /locus_tag="VFF22_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019407340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HZX18081.1"
                     /translation="MKDKSAQDPSTSQLAGTDTLDRGNRNAKLDQLDAFREDATGQAL
                     TTNTGTRIADNQNSLRAGERGPSLLEDFLLREKITHFDHERIPERVVHARGAAAHGYF
                     EVTDPVEDLTCAGFLREAGKRTPVFVRFSTVQGPRGSADTVRDVRGFAVKFYTEEGNF
                     DLVGNNMPVFFIQDAIKFPDFVHAVKPEPHNEIPTGASAHDTFWDFVSLTPESAHMVL
                     WTMSDRAIPIAYRAMQGFGVHSFRLVNAKGESHLVKFHWRPKAGVCSLVWDEAQKLAG
                     KDPDFHRRGLWEDIENGNYPEWELGLQVMDEADQQRFGFDLLDPTKLVPEELVPVRIV
                     GRMVLNRNPDNFFAETEQAAFHVGNIVPGIDFSNDPLMQGRLFSYTDTQLLRLGGPNF
                     NELPINRPLCPFHNNQRDAPHRQQINRGRASYEPNSIDGNWPRETPPAAQQGGFSSYP
                     EPVQGNKVRVRAASFADHFSQASLFWRSMSPPEQEHIIAAYSFELSKVERPQIRERQV
                     KDILQNIDPILASRVAENLGLALLSTPASASAATQPISEALSQTTLLPGDIKGRRVAI
                     LLSAGCNADEVAHVLKGLKGSGAMAKLLGPSLAPVKLHDGASAQPDGTFDTVPSITVD
                     AVFVPRSTDAVQNLQHSGVASHYLMEAYKHLKPIALSGEAAGLAKALGLEEDAGLIVG
                     DNIASLFSRFETALLQHRIWEREMRAQQIPA"
     gene            complement(29179..30201)
                     /locus_tag="VFF22_14625"
     CDS             complement(29179..30201)
                     /locus_tag="VFF22_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HZX18082.1"
                     /translation="MSRIVRFHEFGEADVLKIEERTAPAPAAGEVLIDVEAVGVSWYD
                     VLWRQNLANTPTQLPAGLGHELAGVVLAVGDGVTDLAVGDRVASFPGHSANRYPSYAE
                     HVVLPRSSLARYPANLTAQQAAVHYLPSMVGWFGFNELARLQPGETVLVTAASRCWGP
                     YIVQMGKALGATVIAATAFAEDSDFLKQLGADHIILTEEQDLVSRVSKLTDGRGVNVV
                     MDALGGPQMCLLGDAIAPRGRLILFDLQGGNETPFPACAAFQKNIQFFVHCIGNFTGK
                     EELNIPQDQAAVAKAVQGINQLTADGLLKPLIDKVFSFDDVIEAHRYMETCPSRGRVL
                     LDLGAR"
     gene            30464..31378
                     /locus_tag="VFF22_14630"
     CDS             30464..31378
                     /locus_tag="VFF22_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008053644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX18083.1"
                     /translation="MNRNDLRRVDLNLLIVFETLMHERSVTRAAEKLFLGQPAISAAL
                     ARLRSLFDDPLFVRTGRSMEPTARAMEIAALLSPALDSISTAVSRAADFHPATSNAVF
                     RIGLSDDVEFGLLPPLIKRLRAEAPGVVLVIRRTNYLLMPSLLASGEISVGVAYTDEL
                     PANAKRKVLRRSKPKLLRADAAPGPLSLDDFCARPHALVSFAGDLGGFIDEELEKIGR
                     KRRVVLALPQFNGLGTLLSGTDIIATVPDYTAAALTSAGGVRSEELPLPAQTFELHMA
                     WRGAQDNDPAERWLRSRIQMFCGDPDSL"
     gene            31545..32786
                     /locus_tag="VFF22_14635"
     CDS             31545..32786
                     /locus_tag="VFF22_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZX18084.1"
                     /translation="MEQSNKNLWHFPLALAAVLVLSACGKAPESAQQMPAAQVSVAEV
                     IEQPINEWDEFTGRLEAPESVEVRPRVSGFIDQVAFDEGSLVKKGALLFQIDPRPFQA
                     EVKRLEAQLQQARASQARTQSEANRGERLRQSNAISAELADARASAAAEAKAAVAGIQ
                     AELDNARLNLSFTRVTAPIDGRVSRAEITEGNLVNAGQSLLTTLVSTDKVYAYFDADE
                     RVFLKYVELARQAGGQTRDASPVYLGLSSEDGHPHLGELDFLDNQVNPQTGTIRGRAV
                     FDNRDGRFTPGLYARLKLVGSSQYAAALIKDEAVGTDLGKKFVLVLDKDNAVQYRAVE
                     LGPKLEGLRIVRSGLAKGEKIVVNGLQRAFPGSTVDPQSVPMADEKTQALLTEQRQAI
                     ERQNSRRVAAENSPASTAPRG"
     gene            32800..35979
                     /locus_tag="VFF22_14640"
     CDS             32800..35979
                     /locus_tag="VFF22_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020307456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZX18085.1"
                     /translation="MNFSQFFIQRPIFAAVLSLLILISGAISLFQLPISEYPEVVPPT
                     VVVRATFPGANPKVIGETVASPLEQAITGVEGMLYMSSQGTADGKMTLTLTFALGTDL
                     DKAQVQVQNRVTRTMPTLPTEVQRLGVTVDKASPDLTMVVHLTSPDDRYDMLYLSNYA
                     ALNIKDELARLDGIGDVQLFGLGNYSLRVWLDPNKVASRNLTAADVVNAIREQNRQVA
                     AGSLGAPPSDAGNSFQLSINTQGRLVTEEEFENIIIRAGENGEITRLKDIARIELGSS
                     QYALRSLLNNKPAVAIPVFQRPGSNAIEISDAVRERMAELKQSFPQGVDYEIVYDPTI
                     FVRGSIEAVVHTLLEAIILVVLVVILFLQTWRASIIPLAAVPVSLIGTFAVMHLFGFS
                     LNALSLFGLVLAIGIVVDDAIVVVENVERNIGLGKTPLEATKQAMKEVTGPIVATALV
                     LCAVFIPTAFISGLSGQFYQQFALTIAISTVISAINSLTLSPALAAVLLKSHDAPKDR
                     FSKLLDKLFAGWLFKPFNRAFDRASHGYVGTVKRVLRGSGIALFVYAGLMGLTYLGFA
                     TTPSGFVPAQDKQYLVAFAQLPDAATLDRTEDVMKQMSEIAGKHPGVANTIAFPGLSI
                     NGFTNSPNSGIVFVALKDFDQRKDESMSAGAIAAELNGQFAGIQEAYLAIFPPPPVQG
                     LGTIGGFRLQVQDRGNLGYDELYVQTQNVIAKARQLPELDPMSVFTSYQVNVPQIDAA
                     IDREKAKTHGVAISDIFDTMQIYLGSLYANDFNRFGRTYQVNVQAEQQFRLEPEQIGQ
                     LKVRNNRGEMVPLSAFLKVSASSGPDRVMHYNGFLTAEINGAAAPGYSSGQAEAAMAQ
                     LLKEELPNGMSFEWTDLTYQQILAGNSAIFIFPLCVLLAFLVLAAQYESWSLPLAVIL
                     IVPMTLLSAITGVILAGGDNNIFTQIGLIVLVGLACKNAILIVEFAKEKQEEGMDRVS
                     AVLEACRLRLRPILMTSFAFIMGVVPLVLSSGAGAEMRHAMGVAVFSGMLGVTFFGLL
                     LTPLFYVLIRAFVEKREAQKVARLQEVQA"
     gene            36048..37436
                     /locus_tag="VFF22_14645"
     CDS             36048..37436
                     /locus_tag="VFF22_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZX18086.1"
                     /translation="MKPLGISLLTLALTACAVGPDYQAPQTEAGTLSSAQSSSYDRSR
                     FETSWWQQFDDPVLNQLVQQSLQENRQLRVAFARLLAAQAIRDDLGNDQLPTITSRAS
                     GEFGKAQQPGVTEQRVRGERYDLGLDMLWELDLFGRIQRQLEASDAELDVAEADYQQL
                     QVSLIAELVDAYGSLRGAQLRERIARANLSNQQESRAITEQLREAGVGSQLDVLRSDA
                     RLAGTEASLPQLQAEQQRAKHRIATLLGQRPDQLSVDLSPQELPAIAKALPVGDPGEL
                     LRRRPDIRAAERQLAAATANIGVATADLFPRVSMSGFLGFTAGRGSQLGSSAAQAWGL
                     APTISWAAFDLGSVRARLRGAEAEADGALANYEQQVLLALEESANAFSDYGKRQQRLL
                     ALLRQSDASRAAAEQARVRYREGEVDFLVLLDAERERLSAEDAQAQAEVELYRGIVAI
                     YKALGGGWQPNA"
     gene            complement(37702..38142)
                     /locus_tag="VFF22_14650"
     CDS             complement(37702..38142)
                     /locus_tag="VFF22_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18087.1"
                     /translation="MNRIQFFCWRLLVAATAGVMLFGLVLVAMPGFTQQGFSLLVYAS
                     AAHLNTFGPEALAYISLVHAVLGSVMFGWGAVLMFLALGPVRRGSKDAWWMFVVSLLA
                     WFIPDTAYSLLSGFWQNAVLNAGFALLFAVALGGLRSFIAQTRI"
     gene            complement(38204..38422)
                     /locus_tag="VFF22_14655"
     CDS             complement(38204..38422)
                     /locus_tag="VFF22_14655"
                     /inference="COORDINATES: protein motif:HMM:NF039799.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6500 family protein"
                     /protein_id="HZX18088.1"
                     /translation="MRQDLRDKIISVCTKKILAKDETVGVSFYAFFANKNDDPELLME
                     AARWWIETHKLDHFEKAAKIKSMVESGL"
     gene            complement(38452..38727)
                     /locus_tag="VFF22_14660"
     CDS             complement(38452..38727)
                     /locus_tag="VFF22_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003279732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18089.1"
                     /translation="MKIKVERSKLLRAFNSICAALLMASAIYIIVMGFNSVAMATIAI
                     SVAGAAAPLVQSDEGILELVIGTFEAMIDGVVAVFEGIASAIAGLFS"
     gene            complement(38744..39157)
                     /locus_tag="VFF22_14665"
     CDS             complement(38744..39157)
                     /locus_tag="VFF22_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZX18090.1"
                     /translation="MYTGNCLCGGIKFRIESELEPIQICHCSQCRKAQGTPFATNTPV
                     SLAAFQLISGAELLTIFESSPGKQRVFCSKCGSPVYSKKDSLPGVLRIRAGLLNEELS
                     VKPAAHFYTGSKANWWPISDELPQFEAAYVSSKKA"
     gene            39580..40104
                     /locus_tag="VFF22_14670"
     CDS             39580..40104
                     /locus_tag="VFF22_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter suffix domain-containing protein"
                     /protein_id="HZX18091.1"
                     /translation="MSTEQNTTQPKSAHWRFKLGIAIICLIPVLWLLVPLAAAADLPG
                     SKVAALSGALFILNKILLLMVIAIMGKAGFQELKQHIYGYMSSMVPSTDDEISPLRHR
                     IGLVMFCLPLISSFLEPYIDAVAPGLRPNSWALQMLGDALLIGSFFVLGGNFWEKLRA
                     LFIRTARVTSTDPV"
     gene            40198..41340
                     /locus_tag="VFF22_14675"
     CDS             40198..41340
                     /locus_tag="VFF22_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014338918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HZX18092.1"
                     /translation="MSEALEQPDSTPPPPADTQAAAPAAHPAPPANPLRRIALIVVLV
                     ATALFILSILMERSTPSTSQAVVQGYVVQMASEVGGRVVEIAVLDNARVEAGQLLFRI
                     DPQPYKIAIAEAEARLEQTGQALGANTASVDAAQAQAVSARAQRDNVREQTRRVMQLV
                     ERGVYARARLDEATASLNSAEAAVAKAQADLQRARQELGPTGTDNPQFKQALAQLEKA
                     RLDLVRTEITAPADGVITNLQLAVGQVVSGGQPALTFIDTGTVWISAAFKENSLEHVQ
                     ADDTAELVFDVLPGRVFNARVESVGWGVATEGASSSALPSVRNQSGWVRDPQRFPVRL
                     VVMEPYPKGLRYGSQANVVIYTGDKPLANFFGAITVRLVALLTYVN"
     gene            41369..42370
                     /locus_tag="VFF22_14680"
     CDS             41369..42370
                     /locus_tag="VFF22_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014338917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18093.1"
                     /translation="MARAKRQGLRVALAVTVGFSLGLFNGSVLPFLAPLFATQFLLAS
                     YSPPGLRQGLGTVLLIFIVGALLIFLTGLLGDRPLVLLPLLWLFYFSCFFAQGRQMGG
                     AAPALMLVIAIVVPLLDILQRDLGESIVAILAKAVSVGLLLTWGAHAVFPDTSTADAN
                     AAPQQSPEPGSVTRQAMVSGAILLGMVALCLIDPRLATAMVIPITVASLLSQFELTMS
                     TRAAVGLIALNLLGGVAASLAFTVAEIKPSLWMVTLTVLLVSLLFAGKAVTGTTTGKV
                     FGGGLTTFLILFGLGISPLPGGTPELFSTRIAYVLFAILYALCMASLFWPKPRNASH"
     gene            42403..42747
                     /locus_tag="VFF22_14685"
     CDS             42403..42747
                     /locus_tag="VFF22_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009042086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18094.1"
                     /translation="MRNLWSKYLALLDADLSAKIFDNIKNLLVCALLFAAGTDALRGD
                     HQLFMGLWGSTLAGWGLIIVSAILLLLNISDGLNRLAKMRHHVVLQIILFLLYLILAV
                     RVVEIVWSFRSV"
     gene            complement(42761..43312)
                     /locus_tag="VFF22_14690"
     CDS             complement(42761..43312)
                     /locus_tag="VFF22_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003294468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease"
                     /protein_id="HZX18095.1"
                     /translation="MSSIAFVPLKQVPATFALCAALFTTGAMANQPQIYGLHEHVSLH
                     ELALELPAKLDTGAETASLSAKNIEMFKRDGGEWVRFQLGMKNAKQAQIIEKPLTRMS
                     YIKRRADDRQPGDKQLYTERPVIEMDICVGDELKNIEVNLTDRSAFEFPLLIGSTALK
                     EFNAAVSPGVRYSAGQPSCTQTS"
     gene            43671..43862
                     /locus_tag="VFF22_14695"
     CDS             43671..43862
                     /locus_tag="VFF22_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18096.1"
                     /translation="MEETRQTPVASDAEVLAYMQRQLRSGRVKPSVLVSLTEKEYADV
                     SHERIVQCFNQLDGVLLKR"
     gene            44181..44681
                     /locus_tag="VFF22_14700"
     CDS             44181..44681
                     /locus_tag="VFF22_14700"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HZX18097.1"
                     /translation="MSRPTSLKPLLVLITLACFAMLGGALLLQLLVGWEPCTLCIQIR
                     LWLLCGGVVSLLILAAEALGLRWLTLPLWPLLLAAAGMAVYDNTHLVLIETGVLESSS
                     CSPFPFYAFYLPLHDWLPSVFMSAGVCGQNDYSILGLSFTHWTLLSVALLLALCLFTA
                     WRAARR"
     gene            44998..46005
                     /locus_tag="VFF22_14705"
     CDS             44998..46005
                     /locus_tag="VFF22_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZX18098.1"
                     /translation="MFKSICAIAAGSLLLASTTLHAATDKGPIVIKFAHVVADNTPKG
                     QGALLFKKLAEERLGDKVKVEVYPNSTLYGDANELEALAKNEVQLLAPSLAKFEKYTK
                     QLQVFDLPFLFDDLDAVNRFQKRAKGRQLLRSMEDHNIVGLAYWHNGMKQLSATKALL
                     VPSDAAGLKFRIQPSSVLEAQFNQLGATPLVLPFAEVFDALKSGKVQGAENPWSNIYS
                     KQLHSVQPFITETNHGVLDYMLVSNPRFWYAIPHQIRVQLEGIIDEVTYEVNRQAEAA
                     NQADRDRIAAATGTQVIALSDEQRDAWRQAMQPVWKHFEGVIGADIMKAAQTVNRNKR
                     D"
     gene            complement(46049..>46652)
                     /locus_tag="VFF22_14710"
     CDS             complement(46049..>46652)
                     /locus_tag="VFF22_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZX18099.1"
                     /translation="LERRPDVAAAERRVIAANAEIGVAEAAWYPDLTISASGGYSGSS
                     FADWIDLPNRVWSLGPQLALTLFDGGARSAELERSEAAYDQTVAQYRQSVLDSFREVE
                     DYLVQLRVLQQEAVIQQEALDAARESLRLIENQYRAGTVDFNSVVNVQATALNNERTN
                     LSLLGSRLTASVQLIAALGGGWQIAQLDQQPAEPEQLPAD"
BASE COUNT         9143 a        14129 c        14077 g         9303 t
ORIGIN      
        1 ccgcctgtcg atgaagtccc ccaagagccg cgtgaaccgc tggatgacct cggcctgacc
       61 ggcgcagcca cacagaccga cagcgcgcac gaagacaacg tgaccctcga cgacctcagc
      121 cctgacaccc tattcgacga aagcggcgca cgttcgccga tcgaaccagg cggtgacggg
      181 cctgcggatg aagaactgcg caccgtcgac gagtcggaaa tcggtggcgg catcggtctg
      241 gacgaagcgg agctgggcca ttcagcgccc ttggacggca agccatggac cgatgaggta
      301 tcgcctacag acaaggagcg caagcgctga gtagcgcagt cttcagcgcc actgcctgag
      361 gtgccaggcg cactcagctg tttttgcgca gggcctccag cagctcgctt ttgtccatgc
      421 tggagcggcc tgccaggttg cgtttgcgcg ccaggtccag cagttcggtc ttgttcatgg
      481 cttccagctt ctcacccggc cggggcgtgc ctttacgggt cgctaccgcc cggcgcgccg
      541 acgatttacg cgccgattgc ttggccgtgg cgctggtggt acgcccggag ccgcttttct
      601 cgccgccgcc ggactgttta ttcaccgtgg cccaggcacg agcttcggct tcctcttcgg
      661 gtacgccttt ggccttgtaa ctgtcttcga tgtgctcagc cttgcgtttc tgcttctcgc
      721 tgtaactgtc tttgcttcca cgtggcatga cgcctctcct ggtggtttgc cgcagggccg
      781 gattgccgcg cgacgcttac cgagaagaag cctggaaatg tgtgttggtt ccccgtgtcg
      841 gataagtggc gccgtttcga ttaagcgaac ctctgggcag tttttcacct cagaagaaaa
      901 agcccctctg cggagactcc gatggacgta atcgagcgca tcctggcaac cctggttctg
      961 gagttctctg atttacagca ggtggagcat ctgacacgca ccacgctgcg cttgtttcta
     1021 gccaccttat tgggcgggct gctcggttat gagcgcgaag tcatgggcaa ggcggcgggt
     1081 attcgcacgc acatgctggt gtgtttgggt gcggccatct ttgtcatgcc gattgagcag
     1141 gctggcgccg accatgacgc catgagccgg gtgatccagg gcgtcgccgc cggtatcggt
     1201 tttctctgcg cgggtaccat cctgaaaggc cagaacatct cggacgtcaa ggggctgacc
     1261 acggcagccg gcttgtggac gtctgcagcc ataggcgtcg cggttggcct ggggcgtgaa
     1321 gcgacagccg tattgtcgac cgtgctggtg ctgatggtgc tgcacatcat gccgctgatc
     1381 gttgacccca acccgaacgg taagaacaag gagtaatccg cgccgcaaaa aaccatgaac
     1441 ttttctcgcc gttaccgcct cgcaatacaa caggctgacg ctggttggcc cggcgatcag
     1501 cctgatcgcc gacacccgag aaaaacgtct gcaaggcgtg acaggagcta ggaacaatgc
     1561 cgtcccccgc atccgccaac gaacccattt ttcgtgccct tcagcagccc cgtctggcca
     1621 tcgagaatat tcagccagcc ttggaggcgg ggcgcttcgc ggtgaaatgc ctgtgcggcc
     1681 gcccggtgca agtcagcgcg atcatttttg ccgatgggca tgatcaattg gccgccgatg
     1741 tgctgtggcg cgccaatgac caggcaggct ggcagcgcat tgcgatgcag gcgctgggta
     1801 atgatcgctg gagcgccagc ctgctgatcg agaccatcgg cgcggctgaa tttgtgattg
     1861 aggcctggca cgatgtctac gccagctttc gccatgagct ggggaaaaag tttgcggcag
     1921 gcgcgccggt aagcctggag ttacaagagg gggctgagct ggtgcggcgc atctgtgcag
     1981 gcgcaccagc ctcactgaaa gatcagctga cggagctggt gaaccgcatg gaagccgctc
     2041 agtcgattga agaacgggtc gcgattctgc tcgacccggc gatcagcgag ctgatgcgcc
     2101 ttgccgagta ccggcctcat ctgctgcgca gcgccgttta cccactggat gtcgaacgcc
     2161 gtctggccga atttgccagc tggtatgagt tgtttccgcg ctccgagagc gctgagcccg
     2221 gccaacatgg caccttcgcc gacgtgcacc ggcgtatccc ggacatcgcc cgaatgggct
     2281 tcgatgtgct gtatttcccg ccgattcatc ccatcggcag cacgcaccgt aaggggccga
     2341 acaacagcct gaaggccggg ccggacgatc cgggcagccc ctatgccatc ggcagtcagg
     2401 acggtggcca tgaggccgtg caccctcagc tgggcagcct tgaggatttt cgtgaactgg
     2461 tccgcgtggc tcaccagcac ggcctggagg tggcgctgga cttcgcggtg caatgctccc
     2521 cggatcaccc ctggctgcgc gagcatcccg gctggttcag ttggcgtgcc gacggcagca
     2581 ttcgccatgc ggaaaatccg ccgaaaaaat acgaagacat cgtcaacgtc gagttctacg
     2641 cccccgaggc cattcccagc ctgtggctgg cgctacgaga cgtggtcatg ggctgggtcg
     2701 aactcggtgt gacactgttt cgtgtggaca acccgcacac caagccgatg ccattctggg
     2761 agtggctgat cgcggatgtg cgcggccgta atcctcaggt gatcttcctc gccgaggcct
     2821 ttacccggcc ggcgatgatg gcgcgtttgg gcaaactcgg ttttagccag agctacacct
     2881 atttcacctg gcgcaacagc aaaaccgagt tgcaggacta cttcaccgag ctcaaccagc
     2941 caccctggcg ggactgctac cggccgaatt tcttcgtcaa tacgccggac atcaacccgt
     3001 atttcctgca acgctccggg cgacctgggt ttctcattcg cgccgcgctg gcgaccatgg
     3061 gctctggtct gtggggcatg tacgcaggct tcgagctgtg tgaggcggcg ccggtgcccg
     3121 gcaaagagga gtacctggac tcggagaagt accagttacg ctctcgcgac tatcaggcgc
     3181 ccggcaacat cagcgcgcag atcgcccgct tgaaccagat ccgccgcgat aacccggcat
     3241 tgcaaaccca cctcggcttt caggcctaca ccgtctggaa cgagcagatt ctgtatttcg
     3301 gcaagcgcac ggcggaccgc tccaacttca ttctggtcgc catcagcctc gacccggtaa
     3361 atacccaaga ggcgaatttc gagctgccgc tttgggagct ggggcttgcc gatgacgccg
     3421 acctggaagg cgaagacctg atgaacggcc atcgctggac ctggcacggc aaagtgcagt
     3481 ggatgcgcat tgatccctgg cacctaccgt tcggtatctg gcggatccac tccgctcgct
     3541 gaaaacagga gcacgcagac aatggccaag cgcagtgacg ccaacgcttt tctcaacgat
     3601 ccgcagtggt acaaggacgc agtgatctac caggtccatg tgaagtcatt tttcgatgcc
     3661 aataatgacg gtatcggtga ctttcagggc ctgatcgaga agctcgacta cattgccgag
     3721 ctgggggtca acaccctgtg gttgttgccg ttctacccct cgccgcgccg tgatgacggc
     3781 tatgacatcg ccaagtaccg cggggtacac cccgactacg gcaacctcgc cgatgcccgg
     3841 cgttttatcg ctgaagccca tcgtcgtggc ttgcgcgtaa tcaccgaact ggtgatcaac
     3901 cacacctcgg accagcaccc ctggtttcag cgcgcacgcc acgcgagaaa gggctcaaaa
     3961 gcgcgcaatt actacgtctg gtccgacagc gatgagaagt acgacggcac ccgcatcatc
     4021 tttatcgaca gcgagaaatc caactggacc tgggacgcag tggcgggtca gtacttctgg
     4081 caccgcttct attcgcacca gccggacctc aacttcgata accccgtagt gcttaatgcg
     4141 gtgctggggg tgatgcgttt ctggctggat atgggcgtcg acggtctgcg cctggatgcc
     4201 attccttacc tgatcgaacg cgacggcacc aataacgaaa acctgcccga gacccatgcg
     4261 gtgctcaagc gtatccgcgc cgaactcgac agccgctacc ccgaccggct attgctggcc
     4321 gaggccaacc aatggccgga agacacgcgg ccgtactttg gcgacgacga cgaatgccac
     4381 atggcttttc acttcccgct gatgccgcgg atgtacatgg ccatcgccca ggaagaccgt
     4441 tttcccatca ccgacatcct gcgtcagacg ccggaaattc cgcccaactg ccaatgggcg
     4501 atcttcctgc gcaaccacga tgagctgacc ctggaaatgg tcaccgatga tgagcgcgat
     4561 tacctgtgga actactacgc cgccgatagc cgcgcgcgga tcaacctggg catccgccgc
     4621 cggctggcgc ccctggtctc caaggaccga cggcgcatcg agctgctcaa cagtctgctg
     4681 ctgtccatgc ccggcacgcc gacgctgtat tacggtgacg aaatcggcat gggcgacaac
     4741 atctacctag gtgaccgcga cggggtgcgt acgcccatgc aatggtcggt ggatcgcaac
     4801 ggtgggtttt cccgggccga ccccgccagc ctggttctgc cgcccatcat ggattcgctg
     4861 tatggctatc agaccatcaa tgtcgaggcc caggcgcgcg atcagcattc attgctcaac
     4921 tggatgcggc gcatgctcaa tatccgtaat cagtacaagg ctttcgggcg cggcgccctg
     4981 aaaatgctgg caccgagcaa ccgtcggatt ctgtcctacc tacggactta caccaacgac
     5041 gaagggcatg aagagaacat tctctgcgtg gccaacttgt ccagtgcagc ccaggccgta
     5101 gagctggagc ttgccgatta ccgcgacagg gtgccggtgg aaatgctcgg gggcgcgccc
     5161 tttccgccga tagggcaact gacctatctg ctgacgctgc cgccatacgg gttttactgg
     5221 ttctatctgg cggacgcctc gcagatgccc agctggcatg ccaaacccgt agaacgcatg
     5281 cctgagctgc agaccctggt gctcagccga agcttgagcg aggtgatgaa cgacaggccg
     5341 cggcgcaccc tggagcagga gtcgttaccg ctgtacctgc ccaagcgccg ctggtttgcg
     5401 ggagggcaac ggcctggcgg tgaggtgcgc ctgctgtatg tcatcccgct ggcggagggg
     5461 tcgaatgcgc ccttgctggc cgaggtggcg gtgtcgggtg agcagggcgt cgagcattat
     5521 cggctaccgc ttgcggcagt cgccgagcgc gccatcggca acgatctccc gcaacagctg
     5581 gcattggcac gactacgccg tggccgtcac gtcgagctgc tgaccgatgc cttcagcctg
     5641 ccagccttca gcctgcaggt gttgcgcctg ctgcgcagca atacggtcct gcgcagcgac
     5701 gcgggcgaga tccagtttcg cgccgagccg gcattgttgg agggtgcgca ggtcgatgag
     5761 gcgcaggtca ccttgctatc gaccgagcag tccaacagct cagcgcttat cgctgatcag
     5821 ctgatgctga agctgctacg tcgcggcttc cctggcattc accccgaagt cgagatgggg
     5881 cgtttcctga ctcatcaagg ctacgcaaac gtggcgccat ttctgggtga agtggtgcgg
     5941 gccgacgagg acggtgtacc ccacactctg atggtggcgc aggagtacct gaacaaccag
     6001 ggcgatgcct ggatctggac cctgaacacc ctcgaccgcg ccatacgtga tgagttggcg
     6061 ggcggaacac gcgatcccga gatcgatgcc ctgcgtgagc tcgagatttt ttccgccgtg
     6121 ctcggcaagc gcgtggggga aatgcacgcg gttctcgcgc gcccggtgga gaacccggat
     6181 ttcggttacc aactgagcga cgagcaggac agcgcagcct gggctactgc cattggcgag
     6241 cagctacgcg aggccttgga tgccctgcag caaaaccgcg agcacgtgcc tgcggcgctg
     6301 gtagagcagc tggactggct actggcgcaa aagaaccagc tgctgcatgc gctggacgca
     6361 ctggcgacgc agaccgtcgg cggcctgcgg attcgcgtgc atggcgacct gcacctgggg
     6421 caggtgctgg tggttcaggg ggatgcctac atcattgatt tcgagggtga gcctaatcgc
     6481 cccttggcag agcgccggcg ccggcatagc ccgaccaagg atgtggccgg catgctgcgt
     6541 tccttcgatt acgcggctgc cgtggcgcag cgcaatgcgg gtggggtgga gagcacggcg
     6601 caatcgcagg ccttggtcca tgaaatcagc gcgcgctacc gcaatgcctc acgtacggcc
     6661 tttatggatg cttaccgtct ggccgccggc ggactgcccc atgaatggca cgccagggac
     6721 ggcgagggcg ccgcagtgga tttgttctgc ctggaaaagg cggcatatga agtggtttat
     6781 gaaagcggac atcgccccga ctggatcgat gtgccgctgc agggcctgct ggatctggct
     6841 catcaactca cgggagcgca taggtgaatc aagacagagt agagcgcctg ttacgggccg
     6901 aagacggtga tccgttcagt ttcctcggcc cgcatccgca ggagggcggc acgctggttc
     6961 gggtatggat gcctaacgcc gagggcgtgg agctgctgac ccgcgatgga cagtcgctgg
     7021 gcgccatgca gcgcactgat gcgcaggggc tgttcgagtt gtccataagt caggcgcaga
     7081 gttactgcct gcgtatccag tggccagatg gccaacagga aaccgaagac ccctatgcgt
     7141 tcggcccgtt gctgggggag ctggacctct atctgttcgc cgagggcaat caccgcgaac
     7201 tatggcgctg cctgggcgcg caggttgaga ctcgcgacgg catcagcggg gtacgctttg
     7261 cggtgtgggc cccgaatgcg caacgggttt cggtggtggg caacttcaac agctgggatg
     7321 gtcgccgtca tcccatgcgg ttgcgttatc cggcgggggt ctgggagttg tttatcccgc
     7381 gtctgcaacc gggtgagtgc tacaaatacg agatattgag cgctggtcgc gttctaccac
     7441 tgaaggccga ccccctggcc caggcgactg aagtgccgcc agcaacggga tcacgggtgc
     7501 cgcagcctgc ggctttcgcc tggcaggatc agcaatggat ggaacaccgg cagcaggcgc
     7561 aaggacggca caccgcatta tcgatttacg aactgcacgc gacgtcctgg cagtggcacg
     7621 atgagcgccc gccatcctgg gacgaactgg ctgatcgttt gatcccctat gttcaggacc
     7681 tcggctttag ccatatcgaa ttgatgccca tcatggagca tcccttcggc gggtcgtggg
     7741 gttaccagcc gctgtcgatg tttgcgccca cgtcgcgcct gggcgatgcg caaggtttcg
     7801 ccgcgtttgt tgaccgctgc catcgtgctg ggatcggggt catcctcgac tgggtgccgg
     7861 ggcattttcc caatgatgcg cacggcctgg ccgagtttga cggcaccgcg ctctatgagt
     7921 acgcccatcc gttcgaaggc tttcatccgg actggaatac ctgcatctac aacctggggc
     7981 gtaccgaggt acacggcttt atgctggcca gcgcgctgta ctggttgcgc gaataccaca
     8041 tcgacggtct gcgcgtggat gcggtggcgt cgatgctcta ccgggactat tcccgcgagg
     8101 agggcgagtg gatccccaac cgccatggtg ggcgggaaaa tcttgaggcc atcgaatttc
     8161 tccaacacct caatgaggtg atcagcagtg aagtgccggg ggcgctggtg atcgctgaag
     8221 agtccaccgc ctggcctggc gtcagccgtc cggtgcagga gggcggtctg ggcttcagcc
     8281 acaaatggaa tatgggctgg atgaacgaca gcctgagtta cgtgcagaaa gacccggtct
     8341 atcggatgca ccatcaccac cagatcacct tcggcttgca ttacgccttt tccgaacgct
     8401 acatcctgcc gatttctcat gacgaggtgg tgcacggtaa acgctcgttg ctggggcgta
     8461 tgccagggga tcgctggcaa cagttcgcca acctgcgcct atatttcgcc tttatgtgga
     8521 ctcaccctgg caagaagctg ttgtttatgg gctgtgagtt cggccagtgg cgtgaatgga
     8581 accacgacca gcaactcgac tggtacctgc tgcaataccc cgagcatgct gcgctgcagt
     8641 cgctactgcg cgagctgaat cacctgtacc ggcatgagcc tgctctgcat gggcaagatg
     8701 aatgtgaagc aggctttcag tggctgatcg gtgacgacgc gcgcaatagc gtgtttgcct
     8761 gggtgcgcca tgccgccggg cacccgccgc tgctggtggt gcataacttc acgccccagg
     8821 tgcatgcagc ctaccgggtc ggcgtgtctc gtgcgggggc ttgggaggtg ttgctcaata
     8881 ccgacgagct ccgttggagc ggctccgggg cgggcagtaa aggcgagctg gaaagtgaag
     8941 cggtcaccgc gcatggggaa cgcgattcat tgcagcttga gctaccgccc ctgggcacct
     9001 tgattctgcg tccgcgccag tgagccttca gcctgaagga gccgcagctc cttccaggtt
     9061 ttgctgtgct gaaacactta atgcgcctga gccacgccct gcaacacggt ggcgctggcg
     9121 cgctcattgc gacaggacgc aatgttgccc agtgaaacca cacccacgag gcgtttttct
     9181 cgattgagca ccggcaagcg gcgcatctgg atgtccgcca tgttcttggc cacgtgctcg
     9241 acttcctcat catcgaagca gtaacgcact tcgccactca tcaccttgcg caccggtgtg
     9301 tcgcttgtaa gccctgcagc aacgccgcgg atggcaatat cccggtcggt gaccatgccg
     9361 accaagcgat caccctcatt gaccagcacc gcaccgctat caatgtcagc catcagcgcg
     9421 gcaacgtcac gcaggctgcg ctcgggactc gtggtttgca cgttgcgggt cataatctcg
     9481 ctaattttca tcatttatct cctgtcgaac gtgaaagcct ccggtcgaga ccctctgtac
     9541 attccgaccg gcctcacctg gctgtgttca ggttaattgg cccgccgatg cctaggagta
     9601 gcgcaccgag tggtaggggt aatcacccac gcgcgcgccg acgaccattt cgctgatcca
     9661 attggtgaga atccgcgtat aggcttgctg gtcgctgtcc ttactcaatg catgatcggc
     9721 gccatcgatg atgcgatggg tgagtgagtg ggccttctcg aaagccgcgc ggtagctcat
     9781 cagcgtggtg tgcggaacaa aatcgtcgtg ttccgattcg accagtaata catccccgct
     9841 gaaattcgcg caggccgcga gcgccaggtt gtcttgggct ttccacaggt gccgccggta
     9901 ctgcgccagc ttcgcgacat ccagctgacg tttgggcatg tgccagtctt catcccaata
     9961 cagcgccggc acgcgcaggg ccaaccatct caccgcccgc cgcgcactca agtgggtgga
    10021 cagataggcg ccatagctgc tgccaatcac ggcaatgcag catggatcga cggcggggtg
    10081 gttggccagg cggtcatagg cggcgacgat gtcggtgagg ttgtgctcgc gcgagacgca
    10141 ttccttggcg gcgatggttc gctcatggcc gcgcaggtcg aaggtcaaac acacgcagcc
    10201 taaccctgcg atgcctttgg ctcgcgcgag gtcgcgttgc tgactaccgc cccagccatg
    10261 cacgaacaac acacctggca ccttggtttc cggcgagaga acggtgccag ctatggattc
    10321 gttttccaca atgatctcaa tcgcttcact ttgtgcagtc atgtcttgtt tcagccctgg
    10381 gcgcgagggc tagaggtcgt gccgcctggc tgggcgatag gcgttacctg cacatcggaa
    10441 aactcgttcg gccactcgaa ggtgtggatc gagttgaggc gtaagcgcgc ctgttcaagg
    10501 gtcagctctg cgctttcggc ggcaatctcc acggtatgcg gatgatcgtg cagaaggtaa
    10561 ctgatcagat gcgtaggttt ggacataaca ggcctccagg ttgtatggcg tcaaagttga
    10621 tagacccctg caactgctgc cgcagttcca cctttccgct caacggcaac gcgcgctggg
    10681 aaatcgctca gggcttgcat gaagcagggc gattgctgct ctcgtgctgc tggcgtagat
    10741 gcttggcacg cttttccaga atcaggtagg caacgctcgc gatcagcagc ggtaacagga
    10801 agtacagggc tcgatagccg agcagggcgg cgaccaggct gccctgactg aactgatgct
    10861 gcagcagcgc cagaaataca gcctcaagca cgcccaggcc cgccgggata tgggtaatgg
    10921 cgcctgccac gcagctgatc agcagcacac cgaggaccga cgggtagaac gcgttggcag
    10981 gcagcagcag gtaaatcaat gaagccatca acgcccagtt gcttgcgccc aggcagagtt
    11041 gcaacacggc taggcgaaca gacggcaggg tgatgctgtg ctcgcgccag acccacacgc
    11101 gttgcttggc gaaggcgcaa aaagcgatgt agatggccac gagccccagc agcaaaaagc
    11161 cgatcagctg cagaccggcg gtgcccagct cccagtgctc cggtagctgt atcaggcgca
    11221 gggagaagat cacgccggcc agggccatgt aaccggtcca gttggtcagc agccccaggc
    11281 tgatgattcg cgtcacggtg gaggccttca ggcccaagcg ggtgtatagg cgatagcgca
    11341 acgccacccc gccaacccag gtggtgaaat tcaggttgaa ggcgtaacac accattgcca
    11401 cgggaatcac ctggcgagcg ggcagcttgt ggcgggtgta atagcggccc agcaggtcgt
    11461 acaggctgaa gaccagatag ctgcagaaaa ccagcaatat ccccatcagc agcgtctgcg
    11521 cgctgtagtc gctgagtgcc gcccatacct cgtcccagtc gaggtttttc gccagcaggt
    11581 acagcagcgt cgggatcagc agcaggaaga acacgctcag catgcgcttg ccccagacga
    11641 cccacgcgct ttgctggtgg ctgctcatcc ggtcttttcc tctttgcagt aacagggttt
    11701 gtcttgcgtc tgcgtctgca cctctggcgt cggggtcagc ggcttgaggt tcttgcggtg
    11761 ggccggcagc aagccgggaa tggccgggaa caggcggata aagtgaaagc acagaaaaac
    11821 cagcggggcg cgccaccaat agccgcgcaa gatgcgctcc aggcgaatgc gcgtgcagtg
    11881 ttctgcggac aattgctgca gatggtcata cagctgctga ttgaaggcgc ggtcgctgat
    11941 aaacagattg gcctcaagat tcagcgccag gctcaaggga tcaaggttgc tcgagcccac
    12001 ggtggaccat tcatggtcca ccagggcgac cttgccgtgc agcgggcgct gacagtattc
    12061 atagatgtcc acgccgtcac gcagcaggta gttgtaaagc agccgggaga agccctgcgc
    12121 ccacgccata tccggttgcc cctggagaat cagcgtcacc ttgacgccac gccgcgccgc
    12181 attgcgcagt tcgcgcaata ccttgtagct ggggaagaag taggcattgg ctacgatcag
    12241 ccgttccttg gccgcgcgaa acgcgctcag gtactgctct tcgatatccg tggtgtggcg
    12301 ttcgttgtca cgctccagca gcatgatccg cacatccccg accggcttgg ccaagggctg
    12361 ggttgggctg ggtgcgatga gtgccgagcg cagcaaacgc atggtagaac tgtgcaaatc
    12421 acgcacgatg gggccgctga cttcgacggc gtagtcctgc ttggccatct caccaaaatc
    12481 acccaggtgg tcagccgaat aattgatgcc cccgacgaat gcacgttcac catcgatgac
    12541 cacgatcttg cggtgcaaac ggcggaataa attggtgcgc atacccaggc gcctggggct
    12601 ggggtcgaac acgtgcacct gaataccttc gcgagccatc gccgcgacgt attcgctgcc
    12661 caggtcagcc gtgccatagc catcaaccgc gagcaccaca cgtacgccgc ggcgggccgt
    12721 ctcgatcagc acctgctgca gcgccaggcc aaccttgtcc tccatgatga taaaggtctc
    12781 cagcagcacc tcttcacgag ctgcgcggat acgctcaaac acacgtgggt agaactcctc
    12841 accgttgatc agtaactgga tctggttacc gtcgcgccat acgccgctca tagatggatc
    12901 tccgccgtca ggggggcatg atccgagagg tgcgaccagg gcctggtaga gagcacgacg
    12961 ggggtatggg cggtggcgtt acgcacgtat atgcgatcca ggcagagcag cggaaagcgc
    13021 gcggggaagc ttttcgccgg tgccccatag gttgcgccaa acgcctcgac cagtgattgc
    13081 tcggccagta gcgcatcggc gcgcaggctc cagtcattga agtcgccagc gactatcacc
    13141 ggcgccgtgg cgggcaggga ggcgagcaga tcgaggagca ggctgatctg tttgcgccgg
    13201 tggctatcgc gcaggcccag atgtacgcaa atcgcatgga cttcggcgtg gccgggcacg
    13261 tccagctggc aatgcagcag gccacgttgc tcgtttccct tgaccgtcac atcgaggttg
    13321 ttgtgctcaa cgatggagaa tttcgacagc agcgcattgc catggtggcc gttgggatag
    13381 accgcattgc gcccataggc gaactgcggc cacatgctgt cggcgagaaa ttcatactgg
    13441 ggaatatctg gccaatcgcg aaagcgcttg gcatgcaacg catggctgcc cagcacttcc
    13501 tggaggaaca ccaggtcggc accggttgcc tgcaccgcgc tgcgcaactc cggcaggatg
    13561 aaacgccggt tgagggccgt gaagcctttg tgggtgttga tcgtcatgac ctgcagccgc
    13621 tgcacggtgg taattcggtc gatgaccatc tcaggttggg gaatagtggt actcacgcct
    13681 tcttgctcct tgccgacttc tggccgctcg gggcttgtca ggccaaaccg gcagagcggg
    13741 atggcctggt gggtcagggc gctcaaagct gccccggttt ctccgaccgt gattcgtggt
    13801 agggcgaggc ctcggcgatc acccggtcca gcgcggcgtc gcggacctgc tcttcactgc
    13861 cgctgctggt gcgcttggag ggacgcagtt cgaccttgat ccagccactc tcgcggcggt
    13921 cgaaggcctt gaaggcttcg atggcgtcgt tcatcggttt gacctgtgtg agaatcttcg
    13981 acggatcgat gcgcccggcc tggatcattt cgatcagacg cgggatatag cggcggtggt
    14041 tgcagttgcc catattgatc gtcaggttct tgttcatcgc catgccgatc gggaagtgct
    14101 ccgcctgctg cggatagacg ccgatgatgc ccagtgtgcc ggccttggcc agcccctcga
    14161 ccgcccattg caacgcctgg ctgggtgcat tgccaggtgg ctgccatgcg ttgctgctct
    14221 gggtctgagc ctgctcgctg tcgtggtgac aatgtgcggg gcactcagca tccacgccca
    14281 cggcatcgat ggcgcggtct acgccgatgc cattggtcag gcgacgcagg gtttgcacag
    14341 ggtcttcctg gtcgaagttg atcacttccg cgccttgctg acgggccata tgcaggcggt
    14401 cttcgagccg gtcgatggca aatacccgtg aggcgccgag cagtttggca ctggcaatgg
    14461 cgaactgacc taccgggccg cagccgaaca ccgccaccgt gtcgccgcta ccgatttcag
    14521 ccaactcggc gccgaagtag cccgtgggga agatgtccga aagcaggatc gcctggtcgt
    14581 cactgatctc gctgggcagc ttgaccagcc cgacgttggc ataggggatg cgcgccagtt
    14641 ctgcctgcag cccgtgaaac gcaccggtgg ccgccgggcc gccatagaac gcagtacctg
    14701 cctccttgcc gttggggttg gcctcatcgc attgggcgta gtaacccgcg cggcagtagg
    14761 tgcagttgcc gcaggcaatg gtggagggga cgaccacgcg atcaccgatg cttaaattac
    14821 gcacatcact gcccaaggct tcaacaacgc ccacaccctc gtggccgagg atggtgccct
    14881 tgaccatgcc gctgaccgta cctcggacaa agtgcaggtc agtgccgcag atggccgagg
    14941 cggtcaagcg gatgatggcg tcggtcggtg cctgaatggt gggttcgggt acatcgtcaa
    15001 gacgaatatc gccaatgtcg tgaaaaacca ctgctttcat gtttgcctcc caattccggc
    15061 tggccggtgg cgcaaatgaa tgttgccgtc gagttcggcc catgaatttt cgagtggctc
    15121 aaccagcagg ggttcaggtt atttgtgggc agaaccatga cccatacgag cggcaggcgg
    15181 ccatgcgctt gtcgcgtcag cgttgtagct cgaggagctg caaagagcgg ccggctaatt
    15241 tcagggtggc gtactgcagg ggctgaggtg ccccctctat cacgggttgg tgggtgttga
    15301 tcaggcaact ccagcgcttg cccccagcga cttcgggcag cacaaagctg cgactgtcgt
    15361 ggtaggcgtt gagcaagagc aaaagcgtcg catcagcgcc gctgcgtttc accccggtgg
    15421 gttgggcacg cccatccatc agcatgccca tgcagcgcgc ttcgttatcg tgccagtgcg
    15481 cttcggtcat ttcttcgccg tcaggggcta accaggtgac atccttgacc cccagttcct
    15541 cgttgtaatg ccccaccaga aagcgccggc ggcgcagaat cgggtggctg cggcgcaggg
    15601 caatcaggcg ttggcagaag gcaatcagcg cctcaccttc gtcgtcgata ttccagtcga
    15661 cccagctcag ttcgttgtcc tggcagtagg cattgttgtt gccgtgctga gtgcggccga
    15721 actcgtcgcc ggccagcagc atgggcgtgc cttgggcgaa aaacagggtg gccagcagat
    15781 tgcgcatctg ctgcaggcgc agctggcgaa tcgctgggtc gtccgttgca ccttcacagc
    15841 cgtgattcca cgaacggttg tgatcggtgc cgtcctgatt gttttcgtcg ttggcctcgt
    15901 tgtgtttgtg gtcgtacgaa accacatcgc gcagggtgaa accgtcatgc gcagtgatga
    15961 aatttaccga ggcaaacggg cggcgacccc gctggttata gtgatcgcct gaagcggtaa
    16021 gacggctggc cagttcagca agctcgcctt cgtcgccttt ccagaaggcc cggacgttat
    16081 cgcggaactt gtcgttccat tcggcccagc ccggaggaaa gccgccgacc tgatagccgc
    16141 cggggccgca gtcccagggt tcggcaatca acttgacctt gctcagcacg gggtcctggc
    16201 ggcaggccac aagaaaacca tggcgctcgt cgaagccgtg ggcgtggcgc ccgagaatgg
    16261 tcgccaagtc gaagcgaaag ccatccacgc gcatttcact ggcccagtaa cgcagcgagt
    16321 cggtaaccat ctgcaatacg caggggtggc tgagatccag ggtattgccg gtgccggaat
    16381 cgttgatgta gtagcgccga tcttccggca gcaggcggta atagctggaa ttgtcgatgc
    16441 cgcgcatgca cagggtcggg ccgcgctcgt tgccctcggc ggtgtggttg tagaccacat
    16501 cgagaatcag ctccaaaccg gcggcatgca ggtgcgcaac catttccttg aactcactga
    16561 ccttgccgct ggcgagataa gcagggtggg gcgcaaaaaa gccgatgctg ttgtagcccc
    16621 aatagttgtt catgcctttt tccagcaggt gctggtcgtt gacaaaggca tgcaccggca
    16681 gcaattccag gctcgacaca cccagcgcgc gaatgtgccg caacagctct ggattggtca
    16741 gggcggcaca ggtcccgcga tgagcctcgg gcacggcagg gtggcgcatg ctgatgccgc
    16801 gcaggtgcgc ttcgtagatc agcatgttgt cccacggcac gcgaatgggc gcctgttcgc
    16861 cccaggtgaa ggccgggtcg atcaccttgc atttgggcac gaacgcggcg ctgtcacgct
    16921 catcgaaact caggtcaccg tccggcgagc cgatggtgta accgaacaac gcctcggacc
    16981 attgcaactg ccccaccagt tgcttggcat aggggtcaag cagcagtttg tggtgattga
    17041 agcgatgccc ggcttcgggc tcatacgggc cgtacacgcg atagccgtag atctgcccag
    17101 ggcgggcatc cggtagataa ccatgccaga tctcgtcggt gtattcgggc aactcgatcc
    17161 gctcgacttc ctcgttgccc tggtcatcga aaaggcacag ctccacacgc gtggcatgcg
    17221 ccgagaacag cgcgaaattg acgccgaggc cgtcccaggt ggcgcccagc gggaagggat
    17281 taccttcggt cacgcgcgag tgttgacgat tgcttgcagt gctcatgcgc attactcagg
    17341 cttgggcgtt ttcggggcgc tgctgcgcgg cgcgcgcggc ttcttcagca atgcgggttg
    17401 ctcaggctcg ctgagcggca gtgttttggg tttgctgata cgccgcggtt ttgccggtgc
    17461 tggcgctaac acctcggccg tgttctcggc ctcggccagc ttgcgcgcca tctgccagtg
    17521 acgttcctcc tggccaatgg gtttgccttc cgattcccag atctgaaagg cgaattcacg
    17581 aatgcgttgt tcatcaatgc tcatggtgtg gcgctcctgt agcggcaggg atattgcaaa
    17641 gaacgttgac ggaaaaatcc gtgagcactt gcgcaacggg cagcacctgc gcgcagacgg
    17701 tctggcaggg tgagaggctg cccagcagcg tgagccccga gaactccgcc ggcagcatga
    17761 ggcgggtgtc gccccagtgt ttggccggta tatgggggac ctggtcgtta cccagcagag
    17821 ctgcagtcag gcgcggcacc accaccagca ggtgctcctg gccatggctg cgggtaaacg
    17881 ccaacacatg ctcggcgtgt gtgcccgcga cagtcacggc gcggtagtcg ccgcggctga
    17941 acagcgcggg atactgctgt cgcaggtgca gcacgcgttg tatcaaccac tgcttgatgc
    18001 gcccatcgcg ccactctgcc agcagcgttg cgggattttg taacgcctcc agcgagcgct
    18061 cgcgggcggc aaaattcacc ggcctgcgat tgtcggggtc gaccaggctg aagtcccagt
    18121 actcgcagcc ctgatacagg tccggcacgc caggagtggt caggcgcagc aggcattgca
    18181 cgaggctgtt cagcgctgcc gctggcgcga tgcggttaac cgtgtcggcc agcgcgttga
    18241 gcagctcgcg cccttcgggg gcggtcaaca ggcgcgtgag aaaatccgca caggccgcct
    18301 cgtgctcgct gttgggggca ctccaggcgc tgttgagctt ggcctcgcgc agggcttttt
    18361 cctgccatgc agtcatgcgc tgcaggtagg cctgcagtcc cggctgatca tcggcctgca
    18421 ggtccaaagg ccagctcgcc agcagcgcct ggtagagcat cagctcttcc cctcggctcg
    18481 gggcattcgg ggtggccgcg ctgcgcagcg ctgcagcgtg ttcgctccat tggcgtaccc
    18541 gctcgacgaa ccagggcgcc cgctcactga gcaccgccag ccgggtgcgg ctgtcttcac
    18601 cgcgtttgtg gtcgtgagtg gcggtgctga gcaggttgcg cgggtaatgc ttggcgcgct
    18661 cgaggcagca ctgatgaaag tgcgcggcgg ggcggctgaa gtgctcggcg tcgaaaccga
    18721 cgtcattgcg cgacagcagc acggcgctgc ggtagaacgc cgtgtcttct accgctttgg
    18781 ccgcgattgg ggaggtcagc tgctggaaac gcgtgagcac ctgtgcgcgc agggtgcgcg
    18841 ggcgtcccgg cggcagctcg cgcaatggct caccgccaag ccagcggtcg aggtattcaa
    18901 gcaatggcca gtctgcttcg gccaatgtgc tgtacgcccc gtccagcgcc tgacggaaga
    18961 atttccgatc ctgctccgag cgcccacagg cctgggcgta ggtgcggtat accgggaagt
    19021 gcacgatcag ttcgagcagc gcccggcgaa tcgagcccag ggtcaggtca cgggtgtcga
    19081 tgtcattgcg ggcgacctgc aataggcgct gcgccacttc ttccacatcg ccggccagcg
    19141 agccggtcag caccaggcgc cgggcttcca gcacttcctc ctggaacagc gcgggacggc
    19201 cgctgagggt ctgccacagc tcacgcagcg gctgttcgcc agcagggtcg tgctgcaaca
    19261 acgacacctg gttcataaac tcatagccgg tggtgccgtc cacggcccag tcctgcggca
    19321 ggttttcacc atggccgagg attttttcga catagatcgg gagcgggcgc ggggcttggg
    19381 ctgtgcgcag ggagtcgatg cgccggcgca gacgcttgca ataggtgcgt gggttggcca
    19441 ggccgtctat atggtcgata cgcaagccat cgatcaggcc acgctcgatc agttcgaaaa
    19501 tcttcgcgtg ggttttctcg aatactggtg gacgctctac acgcagcccg gccagttcat
    19561 tgatgtcgaa gaagcgccgc caattgatgt catcggcagc ggtgcgccag ctggcgacgc
    19621 ggtaatgctg gcgctccagc agacggtgca aacgctgctg gctcggcttg tcgacgcgcc
    19681 tgtagcgttc cagcagggcc tccagatgcg tgctctgagc tgtgttttgc gcccattcgg
    19741 ccagctcggc gcacagcgcc tgggccaact cgcgggtgcc ggcgccctga ccgagcgccg
    19801 caaagcgctg ccccagttgt gcgagagcag gctcctcagt tgcgcccaga atgtcgttat
    19861 agctgggcgg cgtaatgggc agacgatgct cgaaatgttc gacgtagaac ctgccgtgct
    19921 gctcgtcgaa gcgcagcgtc agggtggctt cgcgcaaggc ctcgccataa tcactgcgca
    19981 ggaaaggcag cagtaactgg ccttcgagca gggggtcatg ggagttccac tggatatcga
    20041 agaaggtggc gtacgggctg tgtcggcccc attccagaac atccagccac caggtgttgg
    20101 catcgccgcc tacagccata tggttggaga cgatatccag aataaggccc ataccacgcg
    20161 catgcagagc gtctgccagt cgcaccaggg ccgcttcacc acccagttcg gggttgatct
    20221 tgctcgggtc gatcacatca tagccatgca tcgaaccggg gcgggcggtg agcaacggcg
    20281 aggcatacag gtggctgacg cccaggcgct cgaagtaggg gatcagcggc agggcgtcat
    20341 acagggtgaa cccgtggtga aattgcaggc gcaacgtact caacagctca gtcatcggct
    20401 atctcccgcc gcgctgccgc gagacgctcc aagcgctggc gggccagtgg ctgttccagc
    20461 atttcgcgca cgggcagcgc atagcgccgg cgccaattgg ggtgctcgct gccgggccct
    20521 ggcaggttgg gttgctgctc cgccccgcag gcatcttcca gcggcagcaa tgtaagtacg
    20581 gcaggggtgc tgccgatgta accgatgctg gcttgcagac aggcttcgac atcatcgcga
    20641 tcaccctgca actgctcgct ctcggccagg gcatcgacta atgccgagcg ctcacgctcg
    20701 cgctcggaat aatcgctgct gcgctgttcg gcttggctat ggccggcacg cacgcgccag
    20761 tcgatgtcat gcccggcaaa ccagcctttg atgctcggca agtcgtgggt ggaggtggtc
    20821 gccagtgcat tgcgcggcca cttttccggc gcgataaaac gcccttcatg ctgttcaaac
    20881 agcagcaccc gcatgccgag gatgttgcgt gcggccaggg tttcacgcag cccatggggg
    20941 acggtgccga ggtcctcgcc aatcaccagc gcacgatgac ggtgcgattc cagcgccaac
    21001 agacgcagca gatcattcaa cggatattgc agataagccc catcctgcgg atcggcgcct
    21061 tgggggatga cccagagccg ctgtaagccc atgacatgat cgatgcgaat tccaccggca
    21121 tgggccaggt tggcctggag catttcgaga taggcgcgaa aaccatgctg gcgtaatccg
    21181 gctggagaaa aagcgctaac gccccagttc tgcccgttgc gattgaggat gtcggggggc
    21241 gcaccgacgg taacctgggg caagaattca ccctggcgcg accaggcctg gctgcctgcg
    21301 cagtcagcgc ctacggccag atccgctatc agcccgattt tcatgcctgc gccgagagca
    21361 cttgcctggg caccttccag gccatgagcg atcaaccact gggcaaaggc atggtactca
    21421 acacactcgc catactcagc ggcaaaacgg cttacctctg cctgctgcgc atcacgcaaa
    21481 cccgcaggcc aggtgcgcca gtccgtgctc tcaccgttct gctttctgtg ctcataaatg
    21541 gcctcgaagc ggcaatgttg ctgcagcgca ctgccgcctg ccgcacagaa cgcctgaaag
    21601 ctgctgagca gctcgcccgg ggtttgcttg aagtccttgt acagctgacg cagtagccgc
    21661 tgtcgaacct cggcgaccat gggccagtcg ataagctgca gaccttccag gtgcgtcagc
    21721 tcggcctgga caccgcaggc cagtacggcg cgctgtacgc gctcgcgccc cagtatgttg
    21781 gcaggggttg catgcagggc attgaagaac agccgactcg acggcgagta ggggctgtag
    21841 tgctcatagt ccgcgctaaa cagcgcatgt accgggctca gcgccaaggc atcagccccc
    21901 tggttggccg catgccgcgc caattcagcc actgcctggg tatcgcccag gccaccatcg
    21961 cccgggcgac gtagcccata gagctgagcg gtgagccccc ataccgcttg cttgccgcat
    22021 agctgctcgg ccgagaggca ggcggcaggg gtaactgcga gccgatagtg attatcagca
    22081 atgtccaact gaaagtagcc cggtcgatca atggcgggca gagccgcctc gtgatcaagc
    22141 cgccctgagc gcacgtgtcc gcattcatcg gtaagttgat agggcgtatg ggccggataa
    22201 ttgttcagcg aaaacggctg gccctgattc aggatcaaca gggcatcagc ggcgtgctca
    22261 tcgcgttgct gggccagcgc ggacaagctg gcgcggattt gctccgggct ttgcgccgga
    22321 tatcccaaag cttccagcag gctgcgctgc acctgcgggc tgaccgagtg agggcggcca
    22381 taggcatctg tccagtcgat gcagaggtta gccgcgcgcg ccaactcggc aagctgtgta
    22441 tcgctcatgt cagaagctcc aacgtgacca gggcactgga ggtcggcaac aagccctggc
    22501 ggtactcgtc gtcgttggcc ctgtgtgcgt agagtcgttc ggcgaggggt ggcgcggacg
    22561 ccaggcgtac tgcctgattg ccgaggttca ccgcaattcg cagtattttc tccccaccaa
    22621 tccgccaact ggcaagcacc gcaccatcgc cgatcacctg cgtgcctaaa ctcgaggcct
    22681 gttccaggcc cggcagaatg cgggtgcggc gcacgtggag caggtcttta tagaacgcca
    22741 gccactcttg ctggcgcggc cgctgcgcca gggcgaaatc gggctgggat ctgacaaagg
    22801 ttgagggcgc gttgggatcc ggaatttgtt ggcgtgtatg cggattggca aactgagaaa
    22861 actcggcaaa ttcgttgcgc cggccatcac gcacagcctg agccagatcg tcacgataat
    22921 cgctgaagaa caggaagggg cgtctcgagc cccattcctc gcccataaac agcagcggca
    22981 ccatggggca gagcagcagc agtacggtgg ctgccttcag ggcgtccgaa tcagaaatat
    23041 tgatcaggcg ttcaccgaac gcacggttgc ccacttggtc gtggttctgc aggaagagta
    23101 cgaaggcgtg cggcggcagg tgtccgctgg gctcgccgcg gggcagggca tgcctgttca
    23161 tctgtccctg gtagacaaag ccttgttcga gaaaagtcgc caggttgtga gtaggcgatg
    23221 ccgcgtagtc ggcgtagtag ctttcctgtt cgcctgtgag cagaacatgc aaggtgttgt
    23281 gaccatcgtc gttccattgc gcattgaagc cgttgcccag caggcttgcg ctgttgtttt
    23341 cgttttccag caccaggtgt acgtggtgcg gctgcggcac ggcgctacgg acgcgggagg
    23401 ctaactcgag cagaaattcg tcatcgtcta tggcatgcac ggcatccaga cgcaggccat
    23461 cgcagcggta gtccagcagc cacatcaagg cgttttcaca gaagaaatcg cgcacctgcg
    23521 gccgacgaaa atcgatggcg gcgccccatg gcgtcatgcg gtctgtgcgg aaaaagctgt
    23581 tggcatacaa gccgagataa ttgccctctg ggccgaagtg gttgtagacc acatcgataa
    23641 acaccatcag ccccaaccca tgcgctcgat cgataaggtg cttgagctgt accggggtgc
    23701 cgtagcagga ggctggggca aacggcagaa cgccgtcgta cccccagtta cgctgaccag
    23761 ggaaggcgcc cagcggcatt atctgtaccg cagttacacc gagttcggcc aaatgcgcca
    23821 ggtgttcttc gacctgctga aagccaccga aaagaccgac atgcagctcg tagatcaccg
    23881 tttcgaccca ggtccgaccg ttccagtcgt catggcgcca ggggtatgcc gagtggtcca
    23941 ccagccggct gaaaccatgc acatcacccg cctgctggcg tgaggccgga tccggtacac
    24001 gcagatgctc attgatcaca aagcggtagc tagcatcggg ctggcaaacc aggtcgagac
    24061 ggaaccagcc atcggcttct gcgtgcatcg gttgcggcaa ttggccctgc aactccaccg
    24121 cgacgcggtt gcaggagggg gcccataagg caaagcgcgt ttgcgcatca tccagccgct
    24181 gggcaccatg gctgtacatt agagcgacac cgccgcgtgg gcctcgctca gcaggtgctg
    24241 atagaggcgg tcgtaggggg ttacggattg ctgccagaac agcggcgcgt tcatcgcccg
    24301 gcagcgcatt gcattaagca gttcggggcg ctgatagatg ttgaaggcac gctgtaccgc
    24361 ggcttgataa ctctccacgc tggcctcgtt gaagagaaag cccgtcacgc catcctcgat
    24421 ggtatcggcc aggccaccgg tacgcctggc aatggggagc gaagcaaagc gttgcgcata
    24481 catctggctc aggccacaag gttcgaagcg cgacggcatg aggagaaagt cgctgccggc
    24541 gaatatccgc cgtgcatccg tttcgttgaa gccgatgtgc acgccgacgc ggcctgggta
    24601 acgtttggcc agttcttcca tggccgtttc cagctccgct tcaccgcggc cgattaccgc
    24661 caggcgaccg ccgttattga cgatctgatc ggcgatgcgc agtgtcagat cgataccttt
    24721 ctgctggacc agacgcgaaa ccaccgcgaa cagcggaccg cctggttgca ggccgaacat
    24781 ctgctcgaca taggcggtat tggcctgctt gccctgccat tgatcggcac tgaaaccctg
    24841 aaccagatga gggtcgttgg ccggctccca gctttcgtcg atgccgttaa tgatgccgct
    24901 gaggcggccg gccttaacct tggcccgcag catgccgtcc agcccgcagc cgaagcctga
    24961 tgtggtgatt tcctgcgcat aggtacggct gacagtcgtg acatggctgg cgtagaaaat
    25021 accggccttg agaaacgaca gctggccatg aaactccatg ccttcgatgc cacaggcgtg
    25081 tgcggggata gctaattctg cgctgcactg catgtcacaa agaccctgat aggccaggtt
    25141 gtgaattgtc agcagggtgg gggttttgac ctgctgccaa gtcatgtagg cgggagcgag
    25201 ggccgccggc cagtcgttgg catgcaccag ctccgggcgc caatggatgc cggcgtcgcc
    25261 gcgggctatt tcagcggcag ccagccccag gcttgcgaag cggatatggt tatccggcca
    25321 gtcacggccg ttttggtcgc catagggtga gccggcgcgg tcgaagagcg ccgggtgcag
    25381 aagcacataa acaatcaaac catcagccag gtccaaacgg ccgatcttac aggcgggcag
    25441 ggcagccagg ccctcgacct ggccgacaat tcgcagcgcg cgaccgcttt ccaggacctg
    25501 ccggtaacct ggaatcagca cgcggatatc atgctgcgcc gacagcgcgc gcggtaaggc
    25561 ggccgaaaca tcccccaagc cgcccacctt gatgaggtca gcgaactccg aggtgacgaa
    25621 aagaatccgt ttcctttgtg gctgcaaact ctctaaatgc aaggaaggcg gaaccgaagc
    25681 ggggaatatg ctgtgtgaac gagggggggc gattgctgca acatttccca tgtcggtctc
    25741 catataagtc aggtgcatct aacgttggtc tgccgttgat cggccctgcc gagtgcgggc
    25801 aatcgccaca ccgctatgct ctagcgcaat cacgccgtca gcggttcgtc gcccacctta
    25861 ttgatgacgg gaggcggggc agaaaagttc gacttatttg cagttgcgct cgagcagttt
    25921 tcacgcacgt gtctgattga aggaaagtca gttcagcagc ctgccgctga ccagcgcggc
    25981 aaggccttta tgtaacgggc catgggcttg tggtcagggc aggcaatagc catgtggcag
    26041 tgcgcgaggg ggctagtcac cgggagtctg cgcgttgatc tcctcggcca tttgcagatg
    26101 cttttgcagc ttgggcaggg tttcctgggc aaaagccgct atctctttat cgtcggattc
    26161 gctgccacgg cggaacagtt cgatggtttg tttatgggcc atcacctgat tgttcatgta
    26221 ggccttatcg aacgattctt catcgcgcag cttgagtatc aatgccttgg cctggtttat
    26281 cagttcggca tcatccgaga cgttgagctc cttttgcgat gctagctggt taagggtcaa
    26341 gttggccttg ctgtggtcat cgatcatggt ttgcgcgaag tcttttatcg cttgggatga
    26401 ggctttctca agcgccaacc gggcggtttc gatttccgca atgcccttgg cagacgcctc
    26461 ctcgacaaaa tcttcagcgc tgatcgggtc gacttgatca gccgcatgag cggcgttggc
    26521 caggcagaac gcgaaagccg caatggccag caatgatagg tatttgaacg gctgcatggt
    26581 gaatcctcct gtttgtgggg tgttcactgc ggtagaggcg cgagctgaaa acgaagttca
    26641 gagaaaaatt tactcgccga gccgccgaca aaccgcccat caggttgcag gggctgatgg
    26701 ggaaattctg ttcgaacttc gtgcggacct gagatctcca aattttctgt gggcttgaag
    26761 cacccctaaa aattcaagga gttgcctgat gaaagacaaa tctgcacaag acccttcaac
    26821 ctctcagctg gcgggaaccg atacccttga tcgcggcaat cgcaacgcca aactcgatca
    26881 actggacgcc tttcgcgaag acgcgaccgg ccaggccctg acgaccaata ccggtacccg
    26941 catcgccgac aatcagaaca gcctgcgcgc gggggaacgt ggcccctcgt tgctggaaga
    27001 tttcctcctg cgggagaaaa tcacccattt cgatcatgag cgcatccctg agcgggtcgt
    27061 gcatgctcgc ggcgcggcgg cccatggcta cttcgaagta accgacccgg tagaagacct
    27121 tacctgcgcg ggtttcctgc gcgaggcagg caaacgcacc ccggtattcg tacgcttttc
    27181 caccgtgcag gggcccagag gctccgccga taccgtgcgt gacgtccgtg gcttcgcagt
    27241 caagttctat accgaggaag ggaatttcga tttggtcggc aacaacatgc cggtgttctt
    27301 tattcaggat gccatcaagt ttcccgactt tgtccatgcg gttaagcccg agccgcacaa
    27361 cgagatacct acgggcgcct cggcccacga caccttctgg gactttgtct ccctgacccc
    27421 agaatcggcg catatggtgc tctggaccat gtccgaccgc gcgattccca ttgcctatcg
    27481 cgccatgcag gggttcggtg tgcatagctt tcgcctagtc aacgccaagg gcgagagtca
    27541 tctggtgaag tttcactggc ggcccaaggc cggcgtctgt tccctggtgt gggacgaagc
    27601 gcagaaactg gcgggcaagg accctgactt ccaccgacgc ggcctctggg aagacatcga
    27661 aaacggtaat taccccgagt gggaactggg cctgcaggtg atggatgaag cagaccagca
    27721 acgtttcggt ttcgacctgc ttgacccgac aaaactggtg ccggaagagt tggtgccggt
    27781 gcgcatcgtg gggcgcatgg tacttaaccg taacccggac aatttctttg ccgagaccga
    27841 gcaggcagca ttccacgtgg gcaacatcgt gccgggtatc gacttcagca atgatccatt
    27901 aatgcaggga cgcttatttt cttacaccga tacgcagctg ttgcgacttg gcgggccgaa
    27961 tttcaatgag ttgccgatca accggccact ttgccccttt cacaacaatc aacgtgatgc
    28021 accgcaccgc cagcaaatca accggggcag ggcgtcctac gagccaaact cgatcgacgg
    28081 taattggccg cgcgaaacac cgcctgcggc gcagcaaggc ggattcagtt cgtaccccga
    28141 gccagtgcaa ggcaacaagg tgcgcgtacg cgctgccagt tttgccgatc atttttccca
    28201 ggccagtttg ttctggcgca gcatgagccc gccggaacaa gaacacatca tcgccgccta
    28261 tagcttcgag ctgtcgaaag tggagcgtcc gcagattcgt gaacgtcagg tcaaggacat
    28321 cctgcagaac attgatccga tcctggccag ccgggttgcg gaaaaccttg ggctagcgtt
    28381 actcagtaca cccgcaagcg catccgccgc aacccagcct atttcggagg cactgagcca
    28441 gaccacgctt ttaccaggcg atatcaaggg ccgtcgtgtc gccatcctgc ttagcgcagg
    28501 atgcaatgcc gacgaagtcg cgcatgtgct caaaggactg aaaggctcag gcgcgatggc
    28561 caaactgcta ggcccgtcac tggccccggt caaactgcac gacggagcca gtgcgcaacc
    28621 tgacggcaca ttcgataccg tgccgtcgat taccgtggat gccgtttttg ttccacgcag
    28681 caccgacgct gtgcaaaacc tgcagcacag tggcgtcgcc agccactatc tgatggaggc
    28741 ctacaaacac ttgaagccca tcgcattaag cggggaggct gccggcctgg ccaaggcctt
    28801 ggggctcgag gaggatgcgg ggctaatcgt aggcgacaat attgcttcgt tgttcagccg
    28861 tttcgaaacg gcactgctgc aacatcgcat ctgggagcgg gagatgcgcg cgcagcagat
    28921 tccagcctga tcacgattca ccgcgccacg gcctgctggc caacgcgcgg tgacgcgcat
    28981 atggacgaga cagggagcgc gcagcatgaa catcgaaaag cagcattcac aagcgggacg
    29041 cttatgaaca tttaagacat gacctgtcgt ttaacataat atacattata cgaaccactg
    29101 catgccgagc tgagaaccca gacctacagg attcaccgct ctcgccctaa tccgggcttc
    29161 gtacaatgtg agttatgttt aacgcgcgcc aagatccaac aacactcgcc cacggcttgg
    29221 acaggtttcc atatagcgat gcgcctcgat tacatcgtcg aagctgaaca ccttgtcgat
    29281 cagcggcttg agcaaaccat ctgccgtcag ctgattgatg ccttgcacgg ccttggccac
    29341 tgcagcctgg tcttgtggaa tgttcagctc ttccttgccg gtgaagttgc cgatgcaatg
    29401 cacgaagaac tggatgttct tctggaacgc cgcacaggct gggaatggcg tttcattgcc
    29461 gccttgcaga tcaaacagaa tcagccgacc gcgcggcgcg atggcgtcac ctagcaggca
    29521 catctgcggg ccgccgagtg cgtccatcac cacattgacg ccacggccat cggtgagttt
    29581 gctgacgcgg ctaaccaggt cctgctcctc ggtgaggatg atgtgatcgg cgcccagctg
    29641 cttgagaaag tcgctgtctt cggcaaaggc cgtagcggca attaccgtcg cgcccagggc
    29701 tttgcccatt tgcacgatat acgggcccca gcaacggctt gcggcggtta ccagtacggt
    29761 ttcacccggc tgcaaacgcg ccagctcgtt gaagccgaac cagccaacca tcgacggcag
    29821 ataatgcacg gcggcttgtt gcgcggtcag gttggctgga taacgcgcca gagagctgcg
    29881 cggcaatacc acgtgttctg catagctggg ataacggttg gcgctgtgcc ctgggaagct
    29941 ggccactcgg tcacccacgg ccagatcggt aacgccatcg ccgactgcca gtaccacacc
    30001 ggccaattca tgacccaggc ctgctggcaa ttgcgtcggt gtattggcca ggttctgccg
    30061 ccagagcacg tcgtaccaac taacgcccac ggcctcaaca tcgatcagca cttcgcctgc
    30121 ggccggcgca ggagctgtac gctcttcgat cttgaggaca tcagcctcac caaactcatg
    30181 gaaacgaact atgcgggaca tcgcaaacct cttcggttga cccattcagg tcgcggcagc
    30241 tgtctggcat ttctgcgcta tacaggtgcg gcgacccctt atggccacgg actttatccg
    30301 ggctttgcca gcaattccac cgctcggggc tgatattcac catgcttgtg gatgatgatg
    30361 agccacaaac acatcattta cccgtgccat attgcctggc gacagcattc ggcattagcc
    30421 tgagtcgcgc gcaaaaccgc agccactcca gaatggacac cggatgaacc gtaacgatct
    30481 tcgccgcgtc gatctcaacc tgctgatcgt cttcgaaacg ctgatgcatg aacgcagcgt
    30541 gacccgtgcg gcggaaaaac tgtttctcgg tcagccggcc atcagtgctg ccctcgcccg
    30601 tttgcgcagc ctgttcgatg acccgctgtt cgtgcgcacc ggccgcagca tggagcctac
    30661 tgcgcgggcc atggagatcg ccgccctgct ctcgccggcg ttggattcga tctccaccgc
    30721 cgtcagccgc gccgctgatt ttcatccagc caccagcaat gcagtgttcc gcatcggctt
    30781 gtcggatgat gttgaattcg gcctgttgcc gccgctgatc aagcgcctgc gcgctgaagc
    30841 gcccggtgtg gtgctggtaa tacgccgcac aaactattta ttgatgccgt cgctgctggc
    30901 ctctggcgag atctcggtgg gcgtggccta caccgatgaa ctgccggcca atgccaaacg
    30961 caaggtgctg cgccgtagca aaccgaaact gctgcgcgcc gatgccgcgc ctgggccact
    31021 gagcctggat gatttctgcg cacggcccca cgcgctggtg tcctttgccg gcgatcttgg
    31081 tggctttatc gatgaagaac tggagaaaat cggccgcaag cggcgggtgg tactcgccct
    31141 gccccaattc aacggccttg gcaccctgct ctccggcacc gatattatcg ccaccgtgcc
    31201 ggattacacc gcagccgccc tgacttccgc aggcggtgta cgctccgagg agctgccgct
    31261 tccggcacaa accttcgagt tgcacatggc ctggcgcggc gcccaggata acgacccggc
    31321 ggaacgttgg ctgcgctcac gcatccagat gttctgtggt gatccagaca gcctgtagtc
    31381 ctataagcgc cctccccagg cgctttgctc acatagcgcg ttatcgatac aaccttactt
    31441 cccatcattc tgagtgatac acatcttcga gtcgttcgat tcgtcgcctt agccccgccg
    31501 cagcagactc cgcctcatca caacaccctc tggcggaagc acacatggaa cagtccaata
    31561 aaaacctctg gcattttccc ctggcccttg ccgcagtgct ggtactgagc gcctgcggta
    31621 aagcgccgga aagcgcacaa cagatgcccg ctgcgcaagt cagcgtggcc gaagttatcg
    31681 aacaaccaat caacgaatgg gatgagttta ctggccgcct ggaagcgcca gagtcagtgg
    31741 aagttcgccc gagggtgtcc ggctttattg accaggtggc cttcgatgaa ggcagcctgg
    31801 tgaagaaagg cgccctgctg ttccagatcg acccgcgccc cttccaggcc gaggtcaaac
    31861 gccttgaagc ccagctgcaa caggcccgcg ccagccaggc acgcacccag agtgaagcca
    31921 atcgcggcga gcgtctgcgt cagagcaacg ccatctctgc cgaactggct gacgctcgcg
    31981 ccagcgccgc agcagaagcc aaggccgcag ttgccggcat ccaggcggaa ctggataacg
    32041 ctcgtctcaa cctcagcttt acccgggtta ccgcgccgat cgatggccgc gtcagccgcg
    32101 cggaaatcac cgaaggcaac ctggtcaatg ccgggcaaag cctgctgacc actctggtca
    32161 gcactgacaa ggtttacgcc tatttcgatg ccgatgagcg cgtcttcctt aaatatgtcg
    32221 aactggcgcg ccaggccggc ggccagaccc gcgatgccag cccggtgtac ctgggcctgt
    32281 ccagcgaaga cggccacccg cacctaggcg aactggactt tctcgacaac caggtcaacc
    32341 cgcagaccgg caccatccgt ggccgcgccg tgttcgacaa ccgtgacgga cgcttcaccc
    32401 ctggcctcta cgcccgcctc aagctagtcg gcagcagtca gtacgccgct gcgctgatca
    32461 aggatgaggc ggtcggcacg gacctgggca agaagttcgt attggtgctc gataaggaca
    32521 acgccgtgca gtaccgcgcc gtcgagctgg ggccaaagct cgaaggcctg cgcatcgtgc
    32581 gtagcggttt ggccaagggc gagaagatcg tggttaacgg tctgcagcgc gcgttccccg
    32641 gctccacggt tgatccgcaa agcgtgccca tggccgatga aaagacccag gcgctgctga
    32701 ctgaacaacg ccaggccatc gaacgccaga acagccgccg ggtcgctgca gaaaacagcc
    32761 ccgccagcac tgcgccacgt ggctaatagg taaaagacga tgaatttctc gcaattcttt
    32821 atccagcggc cgatcttcgc cgccgtgctg tcactgttga ttctgatcag tggggccata
    32881 tcgctgttcc agttgccgat cagcgaatac ccggaagtgg taccgcccac cgtggtggtg
    32941 cgcgccacct tccccggtgc caaccccaag gtgatcggcg aaactgtcgc ctcgccgctg
    33001 gagcaggcga ttaccggtgt ggaaggcatg ctctatatgt cgtcccaggg cactgccgac
    33061 ggcaagatga ccctgaccct gaccttcgcc ctcggcaccg atctggacaa agcccaggtg
    33121 caggtgcaga accgcgtaac ccgcaccatg ccgaccctgc ccactgaagt gcagcgtctc
    33181 ggcgtgaccg tcgacaaggc ctcacccgac ctgaccatgg tcgtgcacct gacctcgccc
    33241 gacgatcgtt acgacatgct ctatctgtcc aactacgccg cgctcaacat caaggatgag
    33301 ctggcgcgcc tggatggcat cggtgatgtg caactgttcg gcctcggcaa ctactcactg
    33361 cgcgtgtggc tcgatccaaa caaggtggct tcgcgcaacc tgaccgcggc cgatgtggtc
    33421 aacgccatcc gcgagcagaa ccgtcaggtc gccgccggct ccctcggtgc accgccgtcg
    33481 gatgcaggta acagctttca gttgtcgatc aacacccagg gccgcttggt tactgaggaa
    33541 gagttcgaaa acatcatcat ccgcgccggc gaaaacggcg aaattactcg cctaaaagac
    33601 atcgcgcgca tcgagctggg ctccagccag tacgccctgc gttccttgct gaacaacaag
    33661 cccgccgtcg ccatcccggt attccagcgc cccggctcca acgccatcga gatttccgat
    33721 gcggtccgtg agcgtatggc cgagctgaag cagagcttcc cccagggcgt ggactacgaa
    33781 atcgtctatg acccaaccat cttcgtgcgt ggctccatcg aggcggtggt ccacaccctg
    33841 ctcgaagcca tcatcctggt ggtgctagtg gtgattctgt tcctgcagac ctggcgcgct
    33901 tcgatcattc cgttggccgc tgtgccggtg tcgttgatcg gcaccttcgc cgtgatgcat
    33961 ttgttcggct tctcgctcaa cgcactgtcg ctgttcggcc tggtgctggc catcggcatc
    34021 gtggtggacg acgccatcgt cgtggtggaa aacgtcgaac ggaatatcgg tctgggtaaa
    34081 accccgctgg aagccaccaa gcaggcgatg aaggaagtca ccgggcccat cgtcgccacg
    34141 gccctggtgc tatgcgcggt gtttatcccc accgccttta tctccgggct gagcggacag
    34201 ttctatcaac agttcgccct cacgattgcc atttccacgg tgatctcggc aatcaactcg
    34261 ctgaccctgt cccctgccct ggccgccgta ttgctgaaaa gccacgacgc gccgaaggat
    34321 cgcttctcca agctactcga caagctgttt gctggctggt tgttcaagcc gttcaaccgc
    34381 gccttcgacc gcgccagcca tggctatgtc ggcaccgtga agcgcgtgct gcgcggcagc
    34441 ggtatcgccc tgttcgtcta tgccgggctg atgggcctga cctacctggg ttttgccact
    34501 acgccgagcg gtttcgtccc ggcgcaggac aagcagtacc tggtggcctt cgcccaattg
    34561 ccggacgccg cgacgctgga ccgcaccgaa gatgtgatga agcagatgtc ggaaatcgcc
    34621 ggcaagcacc caggtgtggc caacaccatt gccttcccgg gcctgtcgat caacggcttc
    34681 accaatagcc cgaacagcgg catcgtcttc gttgccttga aagacttcga tcagcgcaaa
    34741 gacgaaagca tgtccgcagg ggcaatcgct gctgagctta acggccagtt cgcgggtatt
    34801 caggaagcct atctggcgat cttcccgcca ccaccggtac aaggcctggg caccatcggc
    34861 ggcttccgct tgcaggtgca ggaccgtggc aacctcggtt acgacgagct gtacgtacaa
    34921 acccagaacg ttatcgccaa ggcgcgtcag ttgccggagc tggacccgat gtcggtgttc
    34981 accagctatc aggtgaatgt gccgcagatt gatgctgcca tcgaccgcga aaaggccaag
    35041 acccacggcg tggcgatcag cgacatcttc gacaccatgc agatctacct gggctcgctg
    35101 tacgccaacg acttcaaccg ctttggccgt acctatcagg tcaacgtgca ggccgagcag
    35161 cagttccgcc tggagccgga gcagatcggc cagctcaagg tgcgtaacaa tcgtggcgaa
    35221 atggtgccgt tgtcggcctt cctcaaagtc agcgccagct caggtccgga tcgggtgatg
    35281 cactacaacg gcttccttac cgccgaaatc aacggtgcgg cagcccccgg ctacagctcc
    35341 ggccaggccg aggcggcgat ggcccagttg ctcaaggaag aactacctaa cggtatgagc
    35401 ttcgagtgga ccgacctgac ctaccagcag attctcgccg gtaacagcgc gatctttatc
    35461 ttcccgctct gcgtgttgct ggccttcctg gtactggctg cccagtacga aagctggagc
    35521 ctgccgctgg cggtgatcct gatcgtgccg atgaccctgc tgtcggccat cactggggta
    35581 attctggcgg gaggcgacaa caacatcttt actcagattg gattgatcgt actggtggga
    35641 ttggcctgta aaaacgccat cctcatcgtc gagttcgcca aggagaaaca ggaagaaggc
    35701 atggaccgcg tcagtgcggt gctagaagcc tgccgcctgc gtctgcggcc gattctgatg
    35761 accagcttcg ccttcattat gggtgtggtg ccgctggtat tgtcctccgg tgccggcgcg
    35821 gaaatgcgcc atgccatggg cgttgcggtg ttcagcggca tgctcggcgt gaccttcttc
    35881 ggcctgctgc tgaccccgct gttctacgtg ctgatccgcg cctttgtgga gaagcgcgaa
    35941 gcgcagaaag tggcgcgtct gcaggaggtg caagcatgat cgccctttac agatcgttcc
    36001 cacgctccag cgtgggaacg ccgcccagga cgctccgcgt ccgcagtctg aagcccctgg
    36061 gtatcagcct gctgaccctg gcactgaccg cttgcgccgt cggcccggac taccaagccc
    36121 cgcagaccga ggccggcaca ctgagcagcg cgcaaagcag cagctatgac cgttcgcgtt
    36181 tcgaaaccag ctggtggcag cagtttgacg acccggttct caaccaactg gtgcaacagt
    36241 ccctgcaaga gaaccgccaa ctgcgcgtgg cctttgcccg cctgctggcg gcccaggcga
    36301 tccgagatga cctcggcaac gatcagttgc cgaccattac cagccgcgcc agtggtgaat
    36361 tcggcaaggc ccagcaaccg ggcgtaaccg agcagcgcgt gcgcggcgaa cgttacgacc
    36421 ttggcctgga catgctttgg gagctggatc tgttcggccg tatccagcgc caactggaag
    36481 ccagcgatgc cgagctggac gtggccgaag cggactacca gcagctgcag gtcagcctga
    36541 tcgctgaact ggtggatgcc tacggcagcc tgcgcggcgc gcaactgcgt gaacgtatcg
    36601 cccgcgccaa cctgagcaac cagcaggaat cgcgggccat taccgagcaa ttgcgtgaag
    36661 ccggcgtcgg tagccagttg gatgtactgc gcagcgatgc acgcctggct ggcaccgaag
    36721 ccagcctgcc gcaactgcag gccgaacagc agcgcgccaa gcatcgcatc gccaccctgc
    36781 taggccagcg tccggatcag ttgagcgtcg atctatcgcc tcaggagctg cctgccatcg
    36841 ccaaagcctt accggttggt gaccccggcg agttgctgcg ccgccggccg gatatccgcg
    36901 ccgccgaacg gcaactggcc gccgccaccg ccaatatcgg cgtggccacg gcggacctgt
    36961 ttccacgggt gagcatgtcc ggttttctcg gctttaccgc cgggcgtggc tcgcaactgg
    37021 gctcgtccgc ggcgcaggcc tggggcctgg ccccgaccat cagctgggcc gcgtttgacc
    37081 tgggcagcgt gcgtgcacgc ctgcgcgggg ctgaagccga agccgatggc gcactggcca
    37141 actatgagca acaagtgctg ctggccctgg aggaatcggc caacgccttt agcgactacg
    37201 gcaaacgcca acagcgcctg ctcgccctgc tgcgccaatc cgacgccagc cgcgccgctg
    37261 ccgaacaagc ccgcgtgcgc taccgcgaag gtgaagtgga ctttctcgtg ctgctcgacg
    37321 ccgaacgcga acggctaagc gccgaagacg cccaggccca ggcggaagtc gagctgtatc
    37381 gcggcatcgt cgccatctac aaagcccttg gcggcgggtg gcagcctaac gcctgacttg
    37441 cttaccgact ctccccccgg ttggcttgac cacccccttc ccgccccacg gcatccgctc
    37501 cttgccgtgg ggctttttta tggcgaagta gcgattgcat ggtttgagtc gataagtcgt
    37561 aggttggcgt tgagcgcagc gaagcccaac aggtagcggc gtacctcgtt gggcttcgtc
    37621 gcgttgctcc tcaacccaac ctactcggac atgcgtatgg gcctcgacgc tcatacaagg
    37681 caaagcacac tcagcgcagg gttatatgcg cgtttgcgca atgaatgagc gtaatccgcc
    37741 aagcgcaact gcaaacagca gcgcaaaccc tgcattcaga accgcgtttt gccagaagcc
    37801 agacagcagc gaataggctg tgtcggggat aaaccaggcg agcagtgaaa ccacaaacat
    37861 ccaccatgcg tctttgctgc ctcggcgcac ggggccaagc gcgagaaaca tcagcacagc
    37921 gccccagccg aacatcaccg agcccagcac ggcatgaacc aggctgatat acgccaacgc
    37981 ctcagggccg aatgtattga ggtgtgcagc ggatgcgtaa accagcaggc tgaacccttg
    38041 ctgggtaaag ccaggcatag ccacaagcac cagcccgaac aacataaccc ctgcggtggc
    38101 tgcaacgagt aaacgccaac aaaaaaactg aatacgattc aaggcttctc catgcggaaa
    38161 attctgcgat gaaaattggg gagctggttg ccacttgagc taatcagagt cctgactcaa
    38221 ccattgactt tatttttgcg gccttctcga aatgatcaag cttgtgggtt tctatccacc
    38281 atcttgcagc ttccataagc agctctgggt catcattttt atttgcgaag aacgcgtaaa
    38341 aagaaacgcc aacagtctcg tccttggcaa gtatcttctt tgtacaaacg ctgatgattt
    38401 tatcccttag gtcttgccgc attaacttga gctacctttt gcttaacgtt tctaactaaa
    38461 caggccagca attgcacttg ctatgccttc gaaaacggct acaactccgt caatcatggc
    38521 ttcgaaggtt ccgattacta gctccagaat cccttcgtcg ctttggacaa gaggcgcagc
    38581 tgctccggca acggagatag ctatagtcgc catagcaaca gagttgaagc ccataacgat
    38641 aatgtaaatt gcagacgcca taagcaaggc cgcacaaatt gaattaaagg ccctcagcaa
    38701 ttttgacctt tctactttaa ttttcatttg cggcaccatt ttattatgct tttttcgatg
    38761 atacataagc cgcctcaaac tgtggtaatt cgtcacttat gggccaccaa ttagcctttg
    38821 atcctgtgta gaaatgtgcc gccggtttga ctgaaagctc ttcattaagt aggcctgccc
    38881 tgatgcgtaa tacgccaggt aaactgtcct ttttgctgta aacaggtgag ccgcacttgc
    38941 tgcaaaatac tcgttgcttg ccaggagatg actcaaaaat cgtaagtaac tctgcgccac
    39001 tgatcagctg aaaagcagct aaggaaaccg gcgtgtttgt ggcgaatggc gtcccttgag
    39061 ctttgcggca ctgtgaacaa tgacatattt gaattggttc aagctcggat tcaatacgga
    39121 atttaattcc gccacataaa caatttccgg tatacaaagg agcctcctgc tctaagagcc
    39181 taactagacg aaattcccgc cgcataacac cacataggat gtcgtataaa gacctggcct
    39241 agcaccatcc ctttgggcaa tccaaaagtt tgccatttgc caaaccgccc cacacaacac
    39301 cagccaaaac ctacgtcttt gccgcaacac tgtctagcag ccttctaaca atccccgcag
    39361 cttcagccca aaccgtgacc atgccaaggc tcggtctagg cacgctaaaa caaatgccct
    39421 cccctaaaaa acgctcagca aaccggcagc gcacagacgt tgagcaaaca ccgcgcaata
    39481 aagctgcgcc acgctggttt tgtgctagat atttagctga tggttttgcc gtttttttaa
    39541 aaccccaaaa acaataatcg caagggacgg tacgagcaca tgagtacaga gcagaacaca
    39601 acccagccca agtccgccca ttggcgcttt aagttgggca tcgcgattat ctgtttgatt
    39661 ccggttttgt ggcttctggt gccgctggcg gcggcagcgg atttgcctgg atccaaggtg
    39721 gcggcgctct cgggtgcgct gtttattctc aataaaattc tcttgctgat ggtcatcgcc
    39781 atcatgggca aggccggctt ccaggaattg aagcagcata tctatggcta tatgtcgtcg
    39841 atggtgccga gcaccgacga tgagattagc ccgctgcgcc atcgcattgg cttggtgatg
    39901 ttttgcctgc cgctgatttc ctcctttctt gaaccctata tcgatgctgt cgcgcctggg
    39961 ctacggccca atagttgggc gctgcagatg ctcggcgatg ccttgctgat cggtagcttc
    40021 tttgtgttgg ggggcaattt ctgggagaag ttgcgcgcgc tgtttattcg taccgcgcgg
    40081 gttaccagca ccgaccccgt ttgagcctga acgacccgac ggcccgcgtc tggcgcgtgg
    40141 gcctcacact gccgagccaa cctttgcgct tgttatatct gccttacagg aatctatatg
    40201 tctgaagcat tagagcagcc tgacagcact cccccgccgc cagccgatac acaggccgcc
    40261 gcccccgctg cccaccccgc tccgccggct aatccgctgc gacgtattgc gttgatcgtg
    40321 gtgctggtgg ccaccgcgct gtttattctt tccattctga tggaacgcag caccccttct
    40381 acctcgcagg cggtggtgca gggttatgta gtgcagatgg cctccgaggt gggtggtcgc
    40441 gtggtggaaa tcgcagtgct cgacaatgcc cgggtggagg ctgggcagct gctgtttcgc
    40501 attgatccgc agccctacaa gattgccatt gccgaagccg aggcccgtct cgaacagacc
    40561 ggccaggcgc tgggggccaa caccgcatcg gtggatgccg cccaggctca agcggtttct
    40621 gcccgcgctc agcgtgacaa cgtgcgtgag caaacacgcc gggtgatgca gttggttgaa
    40681 cgcggcgtct atgcgcgggc gcggttggat gaagccaccg catcgctgaa ttccgccgag
    40741 gccgccgtgg ccaaggcgca ggccgatttg cagcgtgctc ggcaggagct ggggccaacg
    40801 ggtaccgata acccgcagtt caaacaggca ctcgcacaac tggagaaggc ccggcttgat
    40861 ctggtgcgta cggaaattac cgcgccggcc gacggggtga tcaccaatct acagctcgcc
    40921 gttggccagg tggtcagcgg tggccagccc gcgctcacct ttatcgacac cggcaccgtg
    40981 tggatttccg cagcctttaa agaaaacagc cttgagcatg tgcaggccga tgatacggcc
    41041 gagctggtgt tcgacgtgtt gccggggcga gttttcaatg cgcgggtcga gagcgtcggc
    41101 tggggcgttg caacagaagg ggctagctct tctgccctgc cctcggtgcg caaccagagc
    41161 ggctgggtgc gtgacccgca acgctttccc gtgcgcctgg tggtgatgga gccttacccg
    41221 aaaggcctgc gctacggctc acaggccaat gtggtgattt acaccggcga taagccactg
    41281 gcgaatttct tcggcgccat caccgtgcgg cttgtggccc tgctgaccta tgtcaactga
    41341 ggccattacg gcgccctccc caatcgatat ggcgcgtgcc aagcgccagg gcctgcgtgt
    41401 ggccctggcg gtaacggtgg gtttcagcct gggcttgttc aacggcagtg tgctgccctt
    41461 tctcgcaccg ttgttcgcca cccagtttct gctggccagc tacagcccac cgggtttgcg
    41521 ccaggggctg ggcaccgtgt tgctgatttt tattgtgggc gcgctgctga tcttcctcac
    41581 gggcctgctc ggtgatcggc cgttggtgct gctgccgctg ctgtggctgt tttacttcag
    41641 ctgctttttc gctcaggggc gtcagatggg cggcgcggcg ccggcgttga tgctggtgat
    41701 cgccatcgtg gtgccgctgc tcgacattct gcagcgggac ctcggtgagt cgattgtggc
    41761 gattctggcc aaggcggtca gcgtaggcct gttgctgacc tggggcgctc acgcggtatt
    41821 cccggacacc agcacggcag atgcaaacgc agccccacag cagagcccgg agccaggcag
    41881 cgtgacgcgc caggcaatgg tcagcggcgc aattttgctg ggtatggttg cgctgtgttt
    41941 gatcgatccg cgcctggcca ccgcaatggt tatcccgatc accgtcgcct cgttgcttag
    42001 ccagtttgaa ctgacgatga gcactcgagc cgctgtcggg ctgatcgccc tcaacctgct
    42061 gggcggggtt gcggcatccc tggcatttac cgtagccgag ataaagccca gcctctggat
    42121 ggttaccctc accgtgctgc tggtaagcct gttgtttgcc ggcaaggcag taacaggcac
    42181 gacaacgggc aaggtatttg gcggcgggct tacgaccttt ctgatccttt ttggccttgg
    42241 catttcacca ctgccaggcg gcacgcccga gctgttttcc acgcgaattg cctatgtgct
    42301 gttcgcaatt ctgtatgcct tgtgcatggc cagcctgttc tggcccaagc cgcgcaacgc
    42361 cagccactaa ccgctgtact gctgctaatt caggtgccgt ttatgcgcaa tctctggagc
    42421 aagtacctgg cgctgttgga tgcagactta agcgccaaga tcttcgacaa catcaagaat
    42481 ctgttggtct gtgcgctgtt gtttgccgcc ggcaccgatg cactgcgagg cgaccatcag
    42541 ctctttatgg ggctttgggg ctctacactc gccggctggg gactgatcat cgtgtcggcg
    42601 attctgctgt tgctgaatat cagcgatggg ttgaaccggc tggccaagat gcgtcatcac
    42661 gtggtgctgc agatcatctt gtttctgctt tatctgatcc tggccgtgcg cgtggtggaa
    42721 atagtctgga gtttcagaag cgtgtagccc cccgcgccac tcagctggtt tgcgtgcaac
    42781 ttggctgccc agccgagtaa cgcacgccag ggctgactgc ggcgttgaac tctttcagtg
    42841 ctgttgagcc gatcagcagc gggaattcaa aggcgctgcg gtcggtcagg ttgacctcga
    42901 tattcttcag ctcatcaccc acgcagatgt ccatctcaat taccggacgc tcggtataca
    42961 gctgcttgtc gccgggctgg cgatcatcgg cgcggcgttt gatatagctc atgcgcgtta
    43021 gcggcttttc aatgatctgc gcctgcttgg cgttcttcat acccaactga aagcgcaccc
    43081 actcgccgcc atcgcgcttg aacatttcaa tattcttggc gctcagagag gcggtttcgg
    43141 cgccggtatc gagcttggcg ggcagctcta gggccagttc atgcaaagag acatgctcat
    43201 gcaggccata gatttgcggc tggttggcca tcgcgccggt ggtgaacagt gcggcgcaga
    43261 gtgcaaaggt ggccggaacc tgtttaagcg ggacgaaggc gatgctggac ataaaaaact
    43321 ccacagacgt ttaaaaagat gggacaaggt taacggcgct ggctgaattc aaacttaatc
    43381 aagcacttat aaaaactacg ccccgccatg aatgaccatg gcgggggcga agtggtttca
    43441 tctcaggctg gttaagcctc tatgcggatc acttgagatc aaagcgatcc aggttcatcg
    43501 ccctggtcca ggcggcgacc atgtcgagca ggttggcgaa taattcattg ctcagggttt
    43561 ccgggcgttt gctgccatcg ccacaaagca gctgataacg tggcgactat cggcgccctg
    43621 tttgcgccgc aatacacagc ttttgttcac ccaaaatgag cgcttgatcc atggaagaga
    43681 cccgccaaac accagtagcc agcgatgccg aagtgctggc ctatatgcag cgtcaattgc
    43741 gctccggccg agtaaaaccg agcgtgctag tgagcttaac ggaaaaggaa tatgccgatg
    43801 tcagccatga gcgcattgtg cagtgcttca atcagttgga cggtgtattg ctcaaacgct
    43861 gagcaatacg gcttgctata aaccgttcgc cagccggtgc aggttgcttg catccatttg
    43921 gtaagcaacc catttctcaa cggctttgcc gccatggtgc tgatagaact gctcagcgcg
    43981 gttattaccg gcggttcggg aaaacgatcc taggcatcaa tctgggcttg tttgcctatt
    44041 cgctggcgat catccttgcg ctgctggccc tgtatttgta tcgctcaaac aggcctgcaa
    44101 cttaactctg caccgcacgt tttagatgcc tgcacagtat ctgccttggc caatgcgccc
    44161 ggtccctttt ggagaatcag atgtcccgcc ccacttcgct caagcctctg ctcgtcctga
    44221 ttaccctcgc ctgctttgcc atgctcggcg gtgccttgct gctgcagctc ctggtaggtt
    44281 gggagccttg caccctgtgc attcagattc gcctgtggct gctgtgtggt ggcgttgtaa
    44341 gcctgctgat tctggcggcg gaagccctcg gcctgcgttg gctgacctta ccgctctggc
    44401 cgttattgct ggccgcggca ggtatggcgg tgtatgacaa cacgcacctg gtgctgatcg
    44461 aaaccggcgt gttggaaagc tccagttgct cgcccttccc cttctatgcc ttttacctgc
    44521 cgctgcatga ttggctgccc agcgtattta tgagcgccgg tgtgtgcggg cagaacgact
    44581 acagcattct cggcctgtcg tttacccact ggaccctgct cagcgtcgcc ctgctcttag
    44641 ccctgtgcct gtttacggcc tggcgcgccg cacggcgctg atccccagta aaaatccccc
    44701 tgcaggggtg gcggtaaaac gccacccctg ccctcggtaa gcggctttat gccgatttct
    44761 ccttgagaat ggcgattctc cgccagtcac tgtgcgcttg atcacgctcg tttcgtggcc
    44821 gttcaagccc tgcagccgtg ctgctactct ggcataagca ttgcgataaa tgcggtgcac
    44881 tgcgcacggc aacttatcgt gctgcgcgcc aacctgacag cctgcacccg ccaacccgct
    44941 gaaactgatt ctggcacgcc gtcaacacaa caacataaga acaacaggaa caacgctatg
    45001 tttaagtcga tctgcgctat cgccgctggc agcctgctgc tggcgtctac cacgcttcac
    45061 gcggcaaccg acaaggggcc gatcgtgatc aagtttgccc acgtggttgc agacaacacc
    45121 cccaaaggcc aaggcgcatt gctgttcaag aagctggccg aggaacgcct gggcgacaag
    45181 gtaaaggtcg aggtgtaccc caactccacc ctgtacggcg atgccaatga actcgaggca
    45241 ttggctaaaa acgaggtgca actgctagca ccgtcgctgg ccaaattcga gaaatacacc
    45301 aagcagttgc aggtattcga cctgcccttc ctgttcgatg acctggacgc cgtcaaccgc
    45361 tttcagaaac gcgccaaagg ccggcaactt ctgcgctcca tggaagatca caacattgtc
    45421 ggcctggcct attggcacaa cggcatgaag cagctatcgg caaccaaggc gctgctggta
    45481 ccgagcgacg cagcgggttt gaagttccgt attcagccat ccagcgtact cgaagcgcaa
    45541 tttaaccagc ttggcgccac accactggtg ctgccgtttg ccgaagtctt cgacgcgcta
    45601 aaaagcggca aagtacaggg cgcagaaaac ccctggtcga acatctacag caagcagctg
    45661 catagcgtgc agccgtttat caccgagacc aaccacggcg tgctcgacta catgttggtg
    45721 agcaacccac gcttctggta cgccataccg caccagatcc gtgtgcagct ggaaggcatc
    45781 attgatgaag tgacctacga ggtgaatcgc caggccgaag ccgctaacca ggccgaccgc
    45841 gaccgcatcg cagcagccac cggcactcaa gttatcgccc ttagcgatga gcaacgcgac
    45901 gcctggcgcc aagccatgca gccagtgtgg aaacatttcg aaggcgtgat cggcgccgac
    45961 ataatgaagg ccgcgcaaac ggttaaccgc aataagcgcg attaaccgcc ctggcgttca
    46021 gcatccgtga acgccatgca atacaggctt aatcagcagg cagttgctct ggctcggcag
    46081 gttgttgatc caattgcgcg atctgccaac caccacccag cgcagcgatc agctgcacgc
    46141 tggcggtcaa acggctgccc agcaggctga ggttagtgcg ctcgttgttc agcgccgtgg
    46201 cctgcacatt gaccacgctg ttgaagtcca cggtaccggc gcggtactgg ttttcgatca
    46261 ggcgcaggga ctcgcgggcg gcgtccagcg cctcctgctg gatcacagct tcctgctgta
    46321 gcacgcgcag ttgcaccagg taatcctcca cctcacggaa actgtcgagc accgattggc
    46381 ggtattgcgc cacggtctgg tcgtaggccg cttcactgcg ttccagctcg gcgctgcgcg
    46441 caccgccatc gaacagggtc agcgccagct gcggaccgag ggaccagacg cggttgggca
    46501 gatcgatcca gtcggcaaag ctgctgccac tgtaaccgcc actggcggag atagtcaggt
    46561 ccggatacca ggcggcttct gctacaccga tctcggcgtt ggcggctatt acccggcgtt
    46621 cggcggcggc tacatccggg cggcgctcca gc
//
LOCUS       DASRUM010000218         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_947318, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000218 DASRUM010000000
VERSION     DASRUM010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947318"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..78)
                     /locus_tag="VFF22_14715"
     CDS             complement(<1..78)
                     /locus_tag="VFF22_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18100.1"
                     /translation="MDFAYSPKVQQLRERVTAFMETHVYP"
     gene            513..1553
                     /locus_tag="VFF22_14720"
     CDS             513..1553
                     /locus_tag="VFF22_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZX18101.1"
                     /translation="MDNPVPVSSSVSVAYLQGLLDYLARRGVDSAELLERVQLSPQIL
                     AQRDQRIAASTYLELLGHGVRLTGDEQLGLHLGEAVRPGYYGVLGYLIMSCATLADAL
                     HRQSRYAALVGNLGQVDLADEPPRAGLEPQVAHSWQPLLPQQKRQLSEETLAGWVTFG
                     HWISGLDIPPTEVRFQHSAPADSSEYQRIFRCPVLFDQVDNALVFPKRLLATPLGQAD
                     AQVRLMLDAYADRLLDEIQQGHSVLDRARLELSRQLPDVGADLQQIAARLALSPRTLQ
                     RRLREAGLSFNQLVDETRQQLVLHYLRDPELELAEIAFLVGFSEPGSLARAFRRWTGQ
                     SPGEYRRSLKDH"
     gene            1606..1938
                     /gene="cyaY"
                     /locus_tag="VFF22_14725"
     CDS             1606..1938
                     /gene="cyaY"
                     /locus_tag="VFF22_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003208803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HZX18102.1"
                     /translation="MSLTEARFHDLVDATQQAVEDIFDDSDLDLDLENSAGVLTVRFE
                     NGTQLIFSRQEPLRQLWLAARSGGFHFDYDQASERWICDSSDELLGEMLARITLEQSG
                     ADLEFEEL"
     gene            1919..2125
                     /locus_tag="VFF22_14730"
     CDS             1919..2125
                     /locus_tag="VFF22_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HZX18103.1"
                     /translation="MSSKSSDGAGQEVASPCRRQCCLDDQDICLGCGRTLQEILDWGK
                     ADMVGKRSICAAAEARLQQRNAVP"
BASE COUNT          435 a          689 c          700 g          490 t
ORIGIN      
        1 tggatagaca tgggtctcca taaacgcggt gacgcgttca cgcagttgct gaaccttggg
       61 ggagtaggcg aaatccatgg gcggctacct tctgacgctg agtgtgaatg actgattcgg
      121 tgaggtggaa atgatgctag atcagggact aggatttatc taacctattc tcaccgtgta
      181 taaacattca tcaccgatat atgatccatg cgggtctgtt accgtttcgt tcgcgagccg
      241 cgttgctgcg aaaaatcgcg ccaggctagg cggaggacgc aggcaatggt gttcccttgc
      301 caagtcctcc aacaccgcat ggcgagattt ttcgcgcaat ccgaagggcc gggctagttt
      361 tagcgcgatg ctgcgtttct cgacactcat ttggaacaac caaacttcgt gtctcatgcc
      421 ttgcctcgcg ctaaaactag ctccggcgcg gccgcgaacg aaacgggaac agaccctcgc
      481 tttgcaccca tcgccagcga tcaggagact ggatggacaa ccccgtaccc gtgagcagct
      541 cggtttccgt tgcctacctg caaggtttgc tcgattacct ggcgcgtcgg ggtgtcgaca
      601 gcgctgagct gctggaacga gtgcagctca gcccgcagat cctcgctcag cgagatcagc
      661 gcatcgccgc cagtacctat ctggagctgc tcggccatgg cgtaaggctg accggcgatg
      721 agcagttggg gctgcacctg ggcgaggcgg tgcgacccgg ttattacggc gtgttgggtt
      781 atctgatcat gagctgcgca accctcgccg acgcgctaca tcgtcagtcg cgctacgccg
      841 cgctggtggg taatctgggt caggttgacc tggccgatga gccgccgcgc gccgggctgg
      901 aaccgcaggt ggcgcacagc tggcagccgc tgttgccaca gcagaaacgt cagctcagcg
      961 aggaaaccct ggccggctgg gtaacctttg gccactggat cagcggcctg gatattccgc
     1021 cgaccgaggt gcgcttccag cattcggccc cggcggatag ctctgagtac cagcggattt
     1081 tccgctgccc ggtgctgttc gatcaggtcg ataacgccct ggtattcccc aagcgcctgc
     1141 tcgccacgcc gcttggccaa gccgatgccc aagtgcgcct gatgctcgat gcctatgccg
     1201 atcgcctgct tgacgagatt cagcagggcc atagcgtgct cgaccgcgcg cgcctggaat
     1261 tgtcccgcca gctgccggac gtgggcgccg acctgcagca aatcgccgcg cgtttggcgc
     1321 tcagtccgcg taccctgcag cggcgtttgc gcgaagccgg gttatccttc aaccagttgg
     1381 tggatgaaac ccgccagcag ttggtgttgc attacctgcg cgacccggag ctggaattgg
     1441 cggaaatcgc cttcctggta ggcttcagtg aacccggttc attggcccgc gcgtttcgtc
     1501 gctggaccgg gcagagccca ggcgaatatc gccgtagctt gaaagaccat taatcaccgc
     1561 agcccaagac ttttgcagtc gctgaatttt ttgaggtaag tcgtaatgag tttgaccgaa
     1621 gcccgctttc acgatctggt cgatgccacc cagcaggctg tggaagatat tttcgatgac
     1681 agcgacctgg acctcgacct ggaaaactcc gctggggtgc tgaccgtgcg ttttgaaaac
     1741 ggcactcagc tgatcttcag tcgtcaggaa ccgctgcgtc agctgtggct ggcggcgcgt
     1801 tccggcggct tccacttcga ttacgaccag gccagcgagc gttggatctg cgacagcagc
     1861 gacgagctgt tgggcgaaat gctggcgcgt atcaccctgg aacaatccgg tgccgacctt
     1921 gagttcgaag agctctgacg gcgcgggcca ggaggtcgcc tcgccctgcc gccgccagtg
     1981 ttgtctggat gatcaggata tctgcctggg ctgtggccgc acgctgcagg aaattctcga
     2041 ctggggcaaa gccgatatgg tgggcaagcg cagcatctgc gcggctgccg aagcacgctt
     2101 gcaacagcgt aacgccgtac cttgaccggg cggtcttgtt tatttgacta gctgtggtac
     2161 ggtcagccca agccttgcaa aaccccgtct tagtgatggg gttttgcttt tttcacgatc
     2221 cgtaaagggg acatacccgc accatgacca gcgcaccttc gctcaccatc acccgtcttg
     2281 acctgcagcg cctggagcat ctgctcgaca gcct
//
LOCUS       DASRUM010000219        28124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_449063, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000219 DASRUM010000000
VERSION     DASRUM010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 28124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28124
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449063"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..89)
                     /locus_tag="VFF22_14735"
     CDS             complement(<1..89)
                     /locus_tag="VFF22_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18104.1"
                     /translation="MSGNTYGKLFTVTTAGESHGPALVAIVDG"
     gene            252..1247
                     /locus_tag="VFF22_14740"
     CDS             252..1247
                     /locus_tag="VFF22_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019648221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZX18105.1"
                     /translation="MTRVLLLPLLLCSALLHAAPDVAQSSTAHSSVQQPMRLQTDQGT
                     LHGTLLKPSGTTPVAVALLIAGSGPTDRDGNNPEGGQSDSLKRLAQSLAKQGIASLRY
                     DKRGVAASRAATPDERNLSVERYVSDAVGWAQLLKANPAFDRLILIGHSEGALIASLA
                     AEQAQADALVAIAGSSRPIDQLLREQLAARLPPHLLVQSNQILDRLLAGQFSPKVPKQ
                     LQVLFRPSVQPYLISLLRQNPGEAFARLQIPALIVQGSHDIQVSVADAQALQAAKPDA
                     ELLIIPGMNHMLRISALDLPAQLASYDNPRLPLAQGLGEQIAQFIQRSGKKPASS"
     gene            1312..2103
                     /locus_tag="VFF22_14745"
     CDS             1312..2103
                     /locus_tag="VFF22_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18106.1"
                     /translation="MTDSPEPQTDTDAAPQEVVEPAHPWAELAPEHFSLLRLAALPTD
                     RHTGARPLRFIQLGRVERLGKQHSLLRLTLQLPGQRVRKEQNILEVWADHEQRTVRFG
                     PDSGLQVQPENRGLGRFMLAQGISWARQHFAHYQVANTTLPSKDAFSDEARARRDHCL
                     QAQGFVVEYLDPLKIKAQCGAPRVSSLHGDWHTEKVQIVELLDAASMLQQADQSLREL
                     EVKVRKLEARIDVFKREDGGLRFTITCLVAFCVFQAGLLIWIATR"
     gene            complement(2326..3159)
                     /locus_tag="VFF22_14750"
     CDS             complement(2326..3159)
                     /locus_tag="VFF22_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZX18107.1"
                     /translation="MAQVGTEQTRFWQSRELDGVELLHAHYIEQRFSPHVHEGFVFTV
                     IEEGAQRFRHRGSEHLAPAGSMVLINPDELHTGSKAHDQGWHYRGFYPDNAQVCGVLA
                     ELELCQQGMPSFALSVLHDPQLHRQFVTLHQLLDSQASALQLQTAWREAILLLFQRHA
                     RLPEGLVPGDEPHAVSRAKELLSSQLAAPPSLEQLAQAVSLSPFHFARVFRRATGLPP
                     HSWLMQRRLEQARALLRNGCAPLSVAMQLGFADQSHLSRQFKQAYGVAPGQYRQARAI
                     S"
     gene            complement(3163..3732)
                     /locus_tag="VFF22_14755"
     CDS             complement(3163..3732)
                     /locus_tag="VFF22_14755"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003115565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZX18108.1"
                     /translation="MFKPQLITLQRGGLRIEPMVDADIPALVSLAEANRAELVHMNGT
                     QRLDWYREGLLEQREGRALPFSIRLGEQLVGTTRFADFVSSLPAAEIGWTWLDRSQHG
                     TGLNSTIKYLMLKHAFENWRMVRVQLKTAASNLRSQRAIEKLGAQREGVLRNHRRLAD
                     GRLDDTVLYSITDHDWPTIKQQLEARFSG"
     gene            3960..7436
                     /locus_tag="VFF22_14760"
     CDS             3960..7436
                     /locus_tag="VFF22_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NahK/ErcS family hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HZX18109.1"
                     /translation="MTLSSGLIAAVALIYMAILFAIAFYGDRRAAPMPPKIRAWVYSL
                     SLAVYCTSWTFFGAVGQAAEQLWSFLPIYLGPIIILVFAPWVLQKMVMISKQENITSI
                     ADFIAARYGKSQTLAVVVALVCLVGVLPYIALQLKGIVLGVNLLSGAGAGANGTGAQD
                     TALIVSLVLALFTILFGTRNLDVTEHHRGMVLAIAFESLIKLLAFLAVGAFVTYGLYN
                     GFGDLFSQAKASPELENFWAETVNWPAMMTQTGLAMIAIICLPRQFHVTVVENIEPKD
                     LRLARWVFPAYLLLAALFVVPIALAGQMLLPAGITPDSFVISLPLAEAHPALAMLAFI
                     GGASAATGMVIVASVALSTMISNDMLLPWLLRRQNTERPFEAFRHWMLTARRVSIVVI
                     LLLAYVCYRLLGSNASLATIGQVSFAAIGQLAPAMFGALVWKQANRQGVFAGLFFGAL
                     IWFYTLVMPLVARGLGWPADALPGVQTLLYGPIGFQVDPLTRGVVLSLAGNFLLFAWV
                     SYFSRTRVAEHWQAGRFIGHDLGNKPSNRSMLAVQVGDLLLLANRFVGEDRARQSFMR
                     FAFRQGKAFDPNQTANSDWIAHTERLLAGVLGASSTRAVVKAAIEGREMQVEDVVRIV
                     DEASEVLQFNRALLQGAIENITQGISVVDQSLRLVAWNHRYIELFNYPDGQVYVGRPV
                     AELIRFNAERGLLGSGNIEEHVSKRLYWMRQGTAHTSERLFPNGRVIELIGNPMPGGG
                     FVMSFTDITEFREAERALKEANEGLEQRVAARTFELSQLNQALTEAKGVAEAANQSKT
                     RFLAAVSHDLMQPLNAARLFSAALSHQDDALPADARELVRHLDSSLRSAEDLISDLLD
                     ISRLENGRITPDRHAFVLNNLFDTLGAEFKVLATEHGMDFRLRGSRLRVDSDIKLLRR
                     ILQNFLTNAFRYAKGRVLLGVRRQGQQLRLEVWDRGPGIPEDKRKVIFEEFKRLDSHQ
                     TRAEKGLGLGLAIADGLCRVLGHQLEVRSWPGKGSVFSVTIPLALHAEPPVLLNQPEH
                     NGEALAGIQVLCIDNEDSILTGMHSLLSRWGCQVWTARNRLECEHLLSENVRPQLALV
                     DYHLDDGETGTELMAWLRTRLGEPLPGVVISADGRPELIAQVHAAGLDFLPKPVKPAA
                     LRALLSRHLKLR"
     gene            complement(7437..8369)
                     /gene="prmB"
                     /locus_tag="VFF22_14765"
     CDS             complement(7437..8369)
                     /gene="prmB"
                     /locus_tag="VFF22_14765"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZX18110.1"
                     /translation="MTDATHTYPTRLRSVRDYIRWAVSRFHGEELFFGHGTDNAWDEA
                     RQLVLGALHLPYEIADSYLDCRLEDDEHAHLHALLARRIDERVPTAYLLGEAWFCGMP
                     FIVDERVLIPRSPIAELIEQHFEPWLAMEPARILDLCTGSGCIGIACAYEFPEAEVVL
                     GDLSYEALEVANQNIERHGLDERVYTVQGDGFAGLPGQRFDLIVSNPPYVDAEDFADM
                     PAEYQHEPELGLACGEDGLDLVRRMLAEAADHLTEKGVLIIEVGNSQVHVEALYPEVD
                     FTWLEFERGGHGVFLLAAAQCREHQALFRQRLNG"
     gene            8578..9867
                     /gene="nhaD"
                     /locus_tag="VFF22_14770"
     CDS             8578..9867
                     /gene="nhaD"
                     /locus_tag="VFF22_14770"
                     /inference="COORDINATES: protein motif:HMM:NF038006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HZX18111.1"
                     /translation="MVIDPFSITGLLVLAIFLIGMTLIVFEAQLEMDKFKPAMFMMSG
                     LIVIGAHYAFYDPNGFQFFLHAQSEIKEELFGLIAFMAFMWMVVELLNERNVFTALNG
                     YLMRKGLGARGMFWATGALSALLSPFLNNITTAMIFGKTVKNISTNQRYTHISLCNIV
                     VASNSGVWFLGTSTSLMVVLAGKISIAGLLLLIPSALIGWLLFAATLHFFYLRKLDGQ
                     SLIQVADQAESALKPGGAGLAIVGFFAVFGAVLCNIVLKVSIEFAIGIGLGIVALYAW
                     LLMRRGIELPWQDQLQKVEWNALLFFIGIITSVACLNHVGWLSYISRLFEVMHPTAVN
                     VVLGVASGVMDNVPVEAAALMSNPQLGLDQWALNALMVGIGGSLTVVGSAAGVMAMSL
                     DKTYSFAVHLKFLPAILVNFFGSLGIWYLQFELFGLY"
     gene            10192..10788
                     /locus_tag="VFF22_14775"
     CDS             10192..10788
                     /locus_tag="VFF22_14775"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HZX18112.1"
                     /translation="MSAPKTMFALTGRSHSPASLNNATLLVIDAQEEYRSGVLQLPGL
                     DAALEEIAKLLSAARAQGSAIVHVKHLGIPDGLLDPRGPRGLHLPEAAPLPGEIIVEK
                     RMPNAFNGTDLHERLQALGHLDLIICGFMTHSSISTTVRATKDYGYRCTVVDAACATR
                     DLPTLDGQIISAAEMHRAEMIALADNFAAVVPRASALI"
     gene            10912..11232
                     /locus_tag="VFF22_14780"
     CDS             10912..11232
                     /locus_tag="VFF22_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017339763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18113.1"
                     /translation="MKHSDGFDARRLRPRNASNWRLRLGAGLAALFATFGVLLSMAGT
                     AALLGRPAALGPLNESPAAASIVVISGLLLLSFGVMLWRRCRRRLRRPSDLSMAPHLM
                     KKRD"
     gene            11293..11850
                     /locus_tag="VFF22_14785"
     CDS             11293..11850
                     /locus_tag="VFF22_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003192796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HZX18114.1"
                     /translation="MQDDDFSLFKAELRGVKPIKHDRADTGKPKPNRAQLATLRQAAT
                     ARVESVKVDGLSDQFVIDVGAEDPLYWAANGVQEGQMRKLKLGQISFEGSLDLHGMTL
                     EKARDTLWEFIAEANKLEVRCVRITHGKAVRMDGRKPMIKSHVNTWLRQHPQVLGFTS
                     CLAKHGGTGAVYVMLKRTMMDGREE"
     gene            11937..12485
                     /gene="folE"
                     /locus_tag="VFF22_14790"
     CDS             11937..12485
                     /gene="folE"
                     /locus_tag="VFF22_14790"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HZX18115.1"
                     /translation="MSLEQNYTAILGQLGEDVSREGLLDTPKRAAKAMQYLCKGYQQT
                     LEEVTNGALFSSDASEMVLVKNIELYSLCEHHLLPFIGKAHVAYIPNGKVLGLSKVAR
                     IVDMYARRLQIQENLSRQIAEAIQQVTGALGVAVVIEAQHMCMMMRGVEKQNSSMVTS
                     VMLGEFRENAATRSEFLSLVNN"
     gene            12880..14475
                     /locus_tag="VFF22_14795"
     CDS             12880..14475
                     /locus_tag="VFF22_14795"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HZX18116.1"
                     /translation="MKKLLLTLLCLSVIGCAKNPEPEKSVDVLLIGGGIMSASLGTYL
                     NELEPGWSIDIYERLDRVADESSNAWNNAGTGHSAFCELNYTPELADGSIDISKAVAI
                     NESFEISKQFWAYQVERKVLNNPPSFINNVPHMSFVWGDDNVDFLKKRHAALQASSLF
                     RGMEYSEDPEQIKQWVPLVMQGREPGQKLAATRVSIGTDVNFGEITRQLFDSLSQQDN
                     VSLNLQHEVRDLERNADNSWNVLVADLANGGAEKTINAKFVFIGAGGAALKLLQKSGI
                     SEGEGYAGFPVGGSFLATSNPEIVAQHQAKVYGMASVGSPPMSVPHLDTRVIEGKKVL
                     LFGPFATFSTKFLKNGSLLDTFSALTTDNILPMTHAGIDNFSLSTYLMSQLMLSQEDR
                     MDSLREYFPEAQSEDWELIQAGQRVQIIKKDAEHGGILQFGTEVVSAADGSLAALLGA
                     SPGASTAAPIMLSVLEKTFKDKLLSPEWQTKLKEIIPSYGQKLNDDLALTNQTRAWSS
                     ERLQLEFVEVPPLALTPEVSQAQ"
     gene            complement(14606..14842)
                     /locus_tag="VFF22_14800"
     CDS             complement(14606..14842)
                     /locus_tag="VFF22_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HZX18117.1"
                     /translation="MKPEDLLLLVTREMPFGKHKGRLLADLPGNYLNWFAREGFPQGE
                     IGRLLALMQEIDHNGLSGLLDPLRTKPRVPFKGQ"
     gene            14966..15337
                     /locus_tag="VFF22_14805"
     CDS             14966..15337
                     /locus_tag="VFF22_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004577078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HZX18118.1"
                     /translation="MIIKALRIGLGQLIIFLDWISRPAKLKRTPQAQAAVEQSASNLA
                     LYQFHACPFCVKTRRTLHKLNVPVALRDAKNDAQARSELEQQGGKIKVPCLRIEENGQ
                     STWLYESKAIIAYLDERFAAA"
     gene            complement(15373..15696)
                     /locus_tag="VFF22_14810"
     CDS             complement(15373..15696)
                     /locus_tag="VFF22_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HZX18119.1"
                     /translation="MKNLLSVIALAYVLASPVFASQHSAAPATNSAAATSQGEVRKID
                     AANQKITLRHGPLTNIGMPPMTMVFQVQDVAQLEGLKVGDKVSFVAQQQGSQFVASEL
                     QAVQP"
     gene            complement(15724..16224)
                     /locus_tag="VFF22_14815"
     CDS             complement(15724..16224)
                     /locus_tag="VFF22_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family protein"
                     /protein_id="HZX18120.1"
                     /translation="MKQTAYLQLAFALAGLLLSGSALGHSEQHAAQHSAAVVKEQKPW
                     GIAGDLEQVSRTIEIGMSDQMRFSPDQLEVKQGETIRFVHRNQGKLLHEFVLGTQADL
                     DEHAAMMVKFPGMQHDEPYMAHVGPGQSGEIIWTFNRAGDFDFACLIAGHYQAGMVGR
                     IRVLAE"
     gene            complement(16237..17649)
                     /locus_tag="VFF22_14820"
     CDS             complement(16237..17649)
                     /locus_tag="VFF22_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HZX18121.1"
                     /translation="MVSRRDFFLGASAITAAVATSAVSRVSLAGIPEAVSQGSADTQP
                     PLQPQNGRPYNPVVTLNGWTLPWRMNNGVKEFHLVAEPVVRELAPGYKAHLWGYNGQS
                     PGPTIEVVEGDRVRIFVTNKLPEHTSIHWHGQRLPNGMDGVSGLTQPAIPVGKTFVYE
                     FVARRPGTFMYHPHADEMTQMAMGMMGFWVTHPKEHHPLISEVDRDFCFLLNAYDIEP
                     GAATPKIMTMLDFNLWTFNSRIFPGIDPLVVRHNDKVRLRVGNLTMTNHPIHLHGHEF
                     EITGSDGGPRPLAARWPEVTTDIAVGQMRQLEFLADEEGDWALHCHKSHHTMNAMGHD
                     VPTLIGVDHREMTRKVAKLIPDYMVMGERGMADMAEMQMSLPDNTAPMMTGDGPFGSV
                     EMGGMFTVLKVRKDQAPGDYRDPGWFKHPAGTVAYEWKGELAKPARSSEAGGQSMPLK
                     QPSGEPVEVQVRKPSGHAGH"
     gene            complement(17666..19027)
                     /locus_tag="VFF22_14825"
     CDS             complement(17666..19027)
                     /locus_tag="VFF22_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZX18122.1"
                     /translation="MVVLLSGCASFSQDGGFTQLEKAGQQLDKQVVWVKTPEQRSQVA
                     ARIAELLAEPLTLDAAVQAALLNNRDLQASFDQLGISEAERVQAGRIPNPGFSFGRME
                     KGSEVEYERGLHINLARLIAMPLTSGMEAQRFEQVQRQTSLALFELASQTRKAWYRAV
                     AAQESLVYMQQVMQAAEASAELARRLAAVGNYSTLQRAQEQTFYADAGLNLIRAEQAR
                     LQSREQLTRLLGLWGEQIDFRLPERLPELPAKPEQLPDVERLAMAQRLDIHAVRLDAE
                     RLASNLGLSKTTRFINVLELGVVNNRSNEEPTQRGYEISVELPLFDWSGAKVAKAEAQ
                     YRQMLNRAAATAINARSQVREAYLGYQASHDIARHYRDEVLPLRARIAEENVLQYNGM
                     FISTFELLADARKQILAVDGYLQAQRDFWIAKADLDMALLGAPSLSSSTPAMAATDEP
                     AGH"
     gene            complement(19065..19397)
                     /locus_tag="VFF22_14830"
     CDS             complement(19065..19397)
                     /locus_tag="VFF22_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18123.1"
                     /translation="MKHFPFAHRQAFICLAWLLPAGSWAESPDPLDAQATTPAVHYQS
                     PLKAYQGFSEQPLHDWREANDLVGRIGGWRTYAQEPYAQESDTQEAPVQQPAEPPTKP
                     MEAHHGHH"
     gene            complement(19520..19936)
                     /locus_tag="VFF22_14835"
     CDS             complement(19520..19936)
                     /locus_tag="VFF22_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18124.1"
                     /translation="MRKTLRTALIWMLMLALPAQSMAAISMQLCAVTQQSGAMAQVDT
                     QMAAHHPGAVVMAQAAEASVHGEHHASDNTSKSMPAADNSLCSLCAFCVGAVALSTAI
                     ASNSPLQAVEPSLARLDQFFGFIAETPERPPRLFLA"
     gene            20049..20450
                     /locus_tag="VFF22_14840"
     CDS             20049..20450
                     /locus_tag="VFF22_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18125.1"
                     /translation="MTSTPPLRQPCPPGACDCGREQLLQQANSDVRILLLTRSEEKRL
                     LERLENLESLADLEKLQRRIYEQLGVRVAVAPGFNEVRSMRGIVIQIDELPGLCRKTR
                     QAIPTAIRRGLEKRPQIAYDLLNAHDLFRDI"
     gene            complement(20576..21454)
                     /locus_tag="VFF22_14845"
     CDS             complement(20576..21454)
                     /locus_tag="VFF22_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZX18126.1"
                     /translation="MRFFPDLQGIKFGTVIKRTVADFIKDELPTYTAALAFQLFFSLF
                     PFLLFLVALISVLNLQPFFDWLRQQATLVMPQTAMELIDGVIEQLQTQKIGLFSLGIL
                     LALWTASAAVRSVMDATNKAYGVKEGRPAWKRLPLSLIYTVVIAATLLVAAGFMVLGP
                     QVLSWLAHYFGIEQLVIEIWTWLRWPLVVGMLMLVVAVVYYVAPDVQQEFRFITPGSV
                     LAVVVWIGASLGFGYYAQNFANYDATYGSIGAIIIFLLYLYISSAVLLFGAEMNAVIE
                     HLSREGKDAGEKSISS"
     gene            complement(21544..23478)
                     /locus_tag="VFF22_14850"
     CDS             complement(21544..23478)
                     /locus_tag="VFF22_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017940077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZX18127.1"
                     /translation="MPSAVQRYKRLLSAALARYFPHTTAYLRAEREAAAPLKKPAPRA
                     KKAAAKAKLDSAKPQSKPRRAASKPRIQGIYGPAMLAVDATQSQAMREQVARAVTAGL
                     LSAPSDEQWAMILINHPLTRIFAGAGSGKSTTLVLRVAFMFCHLGIKPEQLTVISFTN
                     ASCAELREQLLKVLGFFNYPFDATQARQCVRTFHSAMGTVAKSVLDNPRWFEQLDDPQ
                     AASRELDNPLSAGRLRPAQQRLLKQAYQQCYAEQPEFRQRVHSLLQLPMPPAESKRLP
                     KAPLDAYKLPGEFSAASLYEAFYAQAGFAESIGITVAALQPQALTGPAAVREFSEALL
                     LFWPYFERSLQAQGLMTFNGAFAQLTARLNAGGQGVSAQLLAPFGHLLIDEFQDISPQ
                     IVQWLQAVHRTLAGQGGSVSLMAIGDDWQSIYGWRGSSPELFIDFDRHFPGKGKAKRS
                     AVLTLANNYRSVEPIIRDAEALLGGVQHKQAKASQALKATQSGDHGVRLIQRFDSKAR
                     LPELLKQISEQCAYAAGRNSRERNAVLLLSRRNEPLQAITAQLDKKLPVKAYTIHRAK
                     GLQAEVAIILDDCQPPEPHPLRNALYAYSGFFSNSYDQAMQDESLRLAYVAITRGVSR
                     VFWYCQKTQGATRILAGRGR"
     gene            complement(23594..24196)
                     /locus_tag="VFF22_14855"
     CDS             complement(23594..24196)
                     /locus_tag="VFF22_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HZX18128.1"
                     /translation="MYKIYGDYRSGNCYKVKLMLHLLDQPYEWIPIDILKGETQSEAF
                     LAKNPNGKIPVLELEDGTCLWESNAILNFLADGSAFLPTEPRLRTQVLQWQFFEQYSH
                     EPYVAVARFIQLYQGLPEARRTEYEECHVRGHKALKVMEKQLQRTPYLVGENFSIADI
                     ALYAYTHVADEGGFDLTSYSAIRTWLARVASHPRYIGMLD"
     gene            complement(24299..25471)
                     /locus_tag="VFF22_14860"
     CDS             complement(24299..25471)
                     /locus_tag="VFF22_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZX18129.1"
                     /translation="MAFSERVARLKSSLIREILAAAQRPEVMSFAGGLPAEPMLPKVE
                     WADMPTSMGQYGTSEGEPELREAIAEEARALGVPCDASQVLIVSGSQQTLDLASKLFI
                     DPGTEVLLEAPTYLAALQAFQLFGADCIAVPQEADGPQLDALRQRLENHKPAFAYLIP
                     TFQNPSAVRYSEAKRDAVAALLDEFGVTLIEDEPYRELVFDEGSATPIVSRLQKASWI
                     YTGTVSKTLLPGLRVGYLIATPDLYPYLLRLKQSADLHTNRIGQWQALQWLGTRHYRE
                     HLAELRDFYRVRRDAMQDVLAEHFSDLADWQVPQGGLFFWLTLKQPLDTRTLLAPALA
                     QNVAFMPGEPFFIDPDANPGHLRLNFSHVAPERLNEGLKRLAEVIRQAQADAASVV"
     gene            25582..26025
                     /locus_tag="VFF22_14865"
     CDS             25582..26025
                     /locus_tag="VFF22_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZX18130.1"
                     /translation="MLDFKSTATQQTAMEAFFFGYQAFTAKADEMLARRGLSRVHHRI
                     LFFIAKYPGLSMKELLGYLGVSKQALNTPLRQLLEMNLVQSLAAEDDKRKRLLGFTAE
                     GAKLEQALRREQAKLLQRAFSEAGETAVSGWLQVNQTLHNSANEG"
     gene            26175..26786
                     /locus_tag="VFF22_14870"
     CDS             26175..26786
                     /locus_tag="VFF22_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HZX18131.1"
                     /translation="MTTELLLAFIAFAFVTSVTPGPNNMMLLASGVNFGLRRSLPHMF
                     GISLGFMLLVASVGLGLGQLFEQVPLLYSVLRYLGAAYLLYLAWKIANSGAPDSQSNA
                     AGKPFSFLQAAAFQWVNPKAWIMAIGAITTYTPQENFVVNVLLIAALFALVNCPSVGL
                     WTVAGSLLRNWLNNARALRVFNIGMALLLVASLYPIFADTGLL"
     gene            26786..27982
                     /locus_tag="VFF22_14875"
     CDS             26786..27982
                     /locus_tag="VFF22_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016780270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HZX18132.1"
                     /translation="MHDLPQARLRPLADTSTSAVVAGFIAMLTGYTSSLVLMFQAGQA
                     AGLTNGQISSWIWALSIGMAICCIGLSLRYRAPIMIAWSTPGAALLITSLPGVPYSEA
                     IGAYIFASALIVLIGLTGTFDRLMRRIPASIAAALLAGVLFKIGLEICVAAEQQPVLV
                     VAMLLAYLFGKRLLPRYAVLAALIVGTVLAAIFGLLNFEHFELQMAVPEWTTPSFSLA
                     AVISIGIPLFIVAMASQNLPGMAVLRANGYDVPASPLLTTTGLTSMLLAPFGSHGIHM
                     AAISAAICAGPEAHEDPKKRYTAAIWCGVFYGIAGIFGATLAALFAALPKALILSIAA
                     LALFASIIGGLTQAMSEPKEREAALITFLVTASGMTLFSVGSAFWGIVAGLLTLAILN
                     WGKQDA"
BASE COUNT         5480 a         8957 c         8293 g         5394 t
ORIGIN      
        1 cagccatcga caatggcgac caacgccggg ccatggcttt cgccagcggt ggtgacagtg
       61 aacagcttgc cgtaggtatt gccggacatg tgcaaaacgc tccgcgaaaa tcagctccga
      121 ggctacgcca gccggcgcaa aaccccactt gagtaaaggc ggcgagtata cccgtggcgt
      181 ccgtacagat catcctcgaa ccttcagggc ggataaacgt ccaattggct cacccctggc
      241 agtccctatc catgacccga gttcttctgt tgccccttct tctctgcagc gccctgctgc
      301 acgccgcccc cgacgtagca cagagcagca ccgcccacag cagcgtgcag caacccatgc
      361 gcctgcagac agaccaaggc acgctgcacg gcacactgct caagcccagc ggcaccacac
      421 cggtagccgt tgccctgttg attgccggct caggccccac cgaccgtgac ggcaacaacc
      481 ccgaaggcgg ccagagcgac agcctcaagc gcctagccca gagcctggcc aaacagggca
      541 tcgccagcct gcgttacgac aagcgcggtg tggccgccag ccgcgccgcc acaccggatg
      601 agcgcaacct cagtgtcgag cgttatgtca gcgatgcggt gggctgggca cagctgctca
      661 aggccaaccc ggcgtttgat cggctgatac tgatcggcca cagcgaaggc gcgctgattg
      721 ccagcctggc tgccgagcag gcccaggccg atgccctggt ggcaattgcc ggcagttcac
      781 ggccgattga ccagttgctg cgcgagcaac tggccgcgcg cctgccgcca catcttttgg
      841 tgcagagcaa tcaaatactc gaccgcctgc tggccgggca attcagcccc aaggtgccca
      901 agcagttgca ggtgttgttt cgccccagcg tgcagcctta tttgatctcg ctgctgcggc
      961 agaacccggg tgaggccttt gcccgtttgc agatccccgc actgattgtg caaggcagcc
     1021 acgatattca ggtcagcgtc gccgatgctc aggctctaca ggcggccaag ccggatgccg
     1081 agctgctaat catccccggg atgaaccata tgctgcgcat cagcgcgctg gatttaccgg
     1141 cgcaactggc cagctatgac aatcctcggc taccgttggc acaagggctg ggcgagcaga
     1201 tcgcgcagtt catccagcgc agcggcaaaa agccggcctc cagttagccg agcggcggcc
     1261 gataagcgca tcaggcgaca cctgaagatg tcgcgcacag gattgccgtg tatgaccgac
     1321 agcccagaac cacaaaccga taccgacgcc gccccgcagg aagtcgtcga gccagcccat
     1381 ccctgggccg agcttgcccc cgagcacttc agcctgctac gcctggccgc cctgcccacc
     1441 gaccgtcaca ccggtgcgcg gccgctgcgg tttatccagc tgggccgcgt cgagcgtttg
     1501 ggcaaacagc acagcctgct gcgcctaacc ttgcagctgc ccggccaacg cgtgcgcaag
     1561 gagcagaaca tcctcgaagt ctgggccgat cacgagcagc gtaccgtgcg cttcggcccc
     1621 gacagcggcc tacaggtgca accggaaaac cgtggcctgg ggcgttttat gctggcccag
     1681 ggaattagct gggcacgcca gcacttcgcc cactatcaag tggcaaacac caccctgccg
     1741 agcaaagacg ccttcagcga tgaagcgcgc gcccgacgcg accactgcct gcaagcgcag
     1801 ggctttgtgg tcgaatacct cgacccgctg aagatcaagg cgcagtgcgg cgcgccacgg
     1861 gtcagctcgc tgcatggcga ctggcatacg gaaaaggtgc agatcgtcga actgctggac
     1921 gccgccagca tgctgcagca ggccgatcag agcctgcgtg agctggaagt aaaggtacgc
     1981 aagctggaag cgcgtatcga tgtattcaaa cgcgaagatg gcggcttgcg cttcaccatc
     2041 acctgcctgg tggccttttg cgtgtttcag gcgggattgc tgatctggat tgccacgcgc
     2101 tagcagctcc gttgaaaact acctgcgttg ccgatacggc gttaaaagca ggctcagcta
     2161 gccgcttgcg gctaacgcgc ttcagcgcga cccgcagggc gagtgaaacg agtaatgctc
     2221 atttacagct cgtaaactcc gcttcttcgc cgctttgacc agccagaggc tgttactaac
     2281 gtagcgcctt gtctcggctg cctcgccgac gttttcagcg ggctgctaag aaatggcgcg
     2341 ggcctggcga tactgccctg gcgcaacgcc gtaggcctgt ttgaactgcc gactgagatg
     2401 gctctggtcg gcaaagccca gttgcatcgc cacactcagc ggcgcacagc cattccttaa
     2461 taacgcccga gcctgttcca ggcggcgctg catcagccag ctatgcggcg gcaggcccgt
     2521 ggcgcgacga aacactcgag cgaaatgaaa cggcgacaag ctcaccgcct gcgccagttg
     2581 ttccagtgac ggtggcgccg ccagctggct gctgagcaat tccttggcgc ggctgacggc
     2641 atgcggctca tcacccggca ccaaaccctc aggtaaacgc gcatggcgct gaaacagcag
     2701 cagaatcgcc tcgcgccagg cggtctgcag ctgcagcgcc gacgcctggc tgtccaacaa
     2761 ctgatgcaac gtgacgaact ggcgatgcag ctgcggatca tgcagcacgc tcagggcgaa
     2821 tgacggcatg ccctgctgac acagctccag ctcagccaac acgccgcaca cctgcgcgtt
     2881 gtccggatag aagccgcggt agtgccagcc ctggtcatgg gccttggagc cggtgtgcag
     2941 ctcatccgga ttgatcagca ccatgctgcc ggccggtgcc agatgctcgc tgccacggtg
     3001 gcgaaagcgc tgggcaccct cttcgatcac agtgaaaaca aagccttcgt gcacatgggg
     3061 cgaaaagcgc tgctcgatat agtgcgcatg cagcaactcc acgccgtcca actcgcgtga
     3121 ttgccagaag cgtgtttgct cggtacccac ctgcgccatg cctcaaccgc tgaaacgcgc
     3181 ctccagctgc tgcttgatgg tcggccagtc atggtcggtg atgctgtaca gcacggtgtc
     3241 gtccaggcgc ccgtcagcca ggcgacgatg gttgcgcagc acgccttcac gctgcgcgcc
     3301 gagcttttcg atggcgcgtt gcgaacgcag gttgctggcc gcagttttca actgcacacg
     3361 gaccatgcgc cagttctcga aggcatgctt gagcatcagg tacttgatgg tgctgttaag
     3421 gcccgtgccg tgctgactgc ggtccagcca ggtccagccg atttcagcgg caggcaggct
     3481 gctgacaaag tcggcaaagc gcgtggtacc caccaattgc tcacccaggc ggatggaaaa
     3541 aggcaaggcg cgcccctcgc gctgttcgag caggccttcg cggtaccagt ccagacgctg
     3601 ggtgccattc atatgcacca actccgcgcg attggcttcg gccaggctca ctaacgccgg
     3661 gatatcggca tcgaccatgg gctcgatgcg caatccacca cgttgcaggg taatcagttg
     3721 cggcttgaac atagggtccc tcctcggaca gagtcgcagg cgcgacacgc taacagggcc
     3781 ggcaagtgtg cacaatccac aacagcaaca cattctgcgc cagtgcgacc ttggtcgtgc
     3841 ctgacgcacc gtcactttgc tgcgagactg gggcaaatca tatgcagtag gctctgggca
     3901 gccgctgcgc tgccagatat gaccccggcc gccccctata gtttgttttc ggagtttgca
     3961 tgaccctgtc tagcgggctg atcgccgcgg ttgcgctgat ttacatggcc attctgttcg
     4021 ccatcgcctt ctacggcgac cgccgcgccg cgcccatgcc gccgaagatt cgcgcctggg
     4081 tctacagcct gtcactggcg gtgtactgca ccagctggac gtttttcggc gcagtgggcc
     4141 aggccgccga gcagctctgg tcgtttctgc cgatttacct ggggccgatc atcattctgg
     4201 tcttcgcacc ctgggtgctg cagaagatgg tgatgatcag caagcaggaa aacatcacct
     4261 cgattgccga ttttatcgcc gcccgctacg gcaaatccca aaccctggcg gtggtggtgg
     4321 cactggtctg cctggtgggc gtattgccct atatcgccct gcagctcaaa ggcatcgtgc
     4381 tcggggtcaa cctgctcagc ggggctgggg cgggcgccaa tggcaccggc gcccaggaca
     4441 ccgcgctgat cgtgtcgctg gtgctggcgc tgttcacaat attgttcggc acgcgcaact
     4501 tggatgtcac cgagcaccac cgcggcatgg tcctggcgat tgccttcgag tcgctgatca
     4561 agctgctggc ctttctcgcc gtcggcgcat ttgtcaccta cggcttgtac aacggttttg
     4621 gcgacctatt cagccaggcc aaggccagcc ccgaactgga gaatttctgg gcggaaaccg
     4681 tcaactggcc ggcgatgatg acccagaccg gcctggcgat gatcgccatc atctgcctgc
     4741 cgcgacagtt tcatgtgaca gtggtggaga atatcgagcc caaggaccta cgcctggcgc
     4801 gctgggtgtt ccccgcctac ctgctactcg ccgcgctgtt tgtagtgccc attgccctgg
     4861 ccgggcagat gttgctgccc gccggtatca ccccggattc tttcgtgatc agcctgccgc
     4921 tggccgaagc ccatcccgcg ctggccatgc tggcctttat cggcggcgca tcggcggcta
     4981 ccggcatggt gattgtcgcc tcggtagcgc tctccaccat gatttccaac gacatgctgc
     5041 tgccctggct gctgcgccgg cagaatactg agcggccttt cgaggccttc cgccactgga
     5101 tgctcaccgc gcgccgcgtc agcatcgtgg tgatcctgct gctggcctat gtgtgctatc
     5161 gcctgctcgg ctccaacgcc agcctggcga ccatcggcca ggtgtctttc gccgccatcg
     5221 gccaattggc accggcaatg ttcggcgcgc tggtgtggaa gcaggccaac cgccagggcg
     5281 tgtttgccgg gctgttcttt ggcgcgctga tctggttcta caccctggtc atgcccctgg
     5341 tggcgcgtgg cctgggctgg ccagcggatg ccttgccggg tgtgcaaacc ttgctgtacg
     5401 ggccgattgg ctttcaggtc gacccgctga cccgtggtgt ggtgctgtcg ctggccggca
     5461 acttcctgct gtttgcctgg gtctcctact tctcgcgtac ccgggtggcc gagcactggc
     5521 aggccgggcg ctttatcggc catgacctgg gcaacaaacc gagcaaccgc agcatgcttg
     5581 cggtgcaggt cggcgatctg ttgctgctgg ccaaccgctt tgtcggtgaa gaccgtgcgc
     5641 ggcagagctt tatgcgtttc gccttccgcc agggcaaggc cttcgacccc aaccagaccg
     5701 ccaacagcga ctggatagcc cacaccgaac gcctgctggc cggtgtactc ggcgcctcat
     5761 cgacccgcgc ggtggtcaag gcggccatcg aaggccgcga gatgcaggtc gaggatgtgg
     5821 tgcgcatcgt cgacgaagcc agtgaggtgc tgcagttcaa ccgcgcgttg ctgcaaggcg
     5881 ccatcgaaaa catcacccag ggcatcagcg tggtcgacca gtcgctgcgc ctggtggcct
     5941 ggaaccaccg ttatatcgaa ctgttcaact accccgacgg ccaggtgtat gtcggccgac
     6001 cagtggccga actgatccgt ttcaatgccg aacgcggcct gctcggcagc ggcaatatcg
     6061 aggaacacgt cagcaagcgc ctgtactgga tgcgccaggg cactgcgcac acctccgagc
     6121 ggttgttccc caacggccgg gtgatcgagt tgatcggcaa ccccatgccc ggcggcggtt
     6181 ttgttatgag cttcaccgac atcaccgaat tccgcgaagc cgagcgcgcg ctcaaggaag
     6241 ctaacgaagg cctggaacag cgagtagccg ctcgtacttt tgagctgtca caactcaatc
     6301 aggccctgac tgaagccaag ggcgtggcgg aagccgccaa ccagtcgaaa acccgctttc
     6361 ttgccgccgt cagtcatgac ctgatgcagc cgttgaatgc cgcgcggctg ttctccgccg
     6421 ccctgtccca tcaggacgac gccctgcccg ccgatgcccg tgagttggtg cgccatctgg
     6481 acagctcgct gcgctcggcc gaagacctga tcagcgacct actggatatt tcccgcctgg
     6541 aaaacggtcg tatcaccccg gatcgtcacg cctttgtgct gaacaatttg ttcgacaccc
     6601 tcggcgcgga attcaaggtg ctggccaccg aacacggcat ggacttccgc ctgcgcggca
     6661 gccgcctgcg ggtcgacagc gacatcaaat tgctgcggcg gattctgcag aacttcctga
     6721 ccaatgcctt ccgctatgcc aaaggtcgcg tgctgcttgg cgtgcgccgt cagggccagc
     6781 agctgcgact ggaagtctgg gaccgcgggc ctgggattcc cgaggacaag cgcaaagtaa
     6841 ttttcgaaga gttcaagcgc ctggacagcc accaaacccg cgccgaaaag ggcttgggct
     6901 tgggcctggc gattgccgat ggtctgtgcc gtgtgctcgg ccatcagctg gaagtacgct
     6961 cctggccggg caagggcagc gtattcagcg tcaccattcc cctggccttg cacgccgaac
     7021 cacccgtgct gctcaaccag cccgagcaca acggcgaggc gctggccggc atccaggtgc
     7081 tgtgcatcga taacgaggac agcatcctca ccggcatgca tagccttttg tcacgctggg
     7141 gatgccaagt atggacggcg cgcaatcgcc tggagtgcga acacctgctg agtgaaaacg
     7201 tgcggccgca gctggcactg gtggactacc acctggatga cggcgaaacc ggcaccgagc
     7261 tgatggcctg gctacgcacg cgcctgggcg agccgctacc gggtgtagtc atcagcgccg
     7321 acggccgccc cgagctgatc gctcaggtgc atgccgccgg cctcgacttc ctgcccaagc
     7381 cggtcaagcc agctgccttg cgggcgctgc taagccgtca tttaaagtta cgttgatcaa
     7441 ccattcaggc gctgacggaa cagcgcctga tgctcgcgac attgcgcggc cgcgagcagg
     7501 aatacgccgt gaccgccgcg ctcgaattcc agccaggtga agtcgacttc cgggtacagc
     7561 gcctcgacgt gcacctggct attgccaacc tcgataatca gcacgccttt ctcggtcaga
     7621 tgatccgccg cctcggcgag catacgccgc accaggtcca ggccatcttc gccgcaggcc
     7681 aggcctagct caggctcgtg ctggtattcg gccggcatat cggcgaaatc ctcggcatcc
     7741 acgtaaggcg ggttggaaac gatcaggtca aagcgctgcc caggcagccc ggcgaaacca
     7801 tcaccctgca cggtgtagac gcgctcatcc aggccatggc gctcgatatt ctggttggcc
     7861 acttccagcg cttcatagga cagatcaccg agcaccactt cggcctcggg gaactcatag
     7921 gcgcaggcga taccaatgca gcccgagccg gtgcacagat cgagaattcg cgccggttcc
     7981 atggccagcc aaggctcgaa atgctgctcg atcagctcgg caatcggtga gcgcggaatc
     8041 agcacgcgtt catcgacaat aaacggcatg ccgcagaacc aggcctcgcc cagcaggtag
     8101 gcggtcggta cgcgctcgtc gatacgccgt gcgagcaagg catgcaggtg cgcatgctca
     8161 tcgtcttcca ggcggcaatc gaggtagctg tcggcaattt cgtaaggtag atgcagggcg
     8221 cctagcacca gctggcgggc ttcatcccag gcgttgtcgg tgccatggcc gaagaacagt
     8281 tcctcgccat gaaaccgact gaccgcccag cgaatgtagt cgcgcacgct gcgcaggcgg
     8341 gttgggtagg tatgtgtagc gtcggtcacg gcagctctcc gcagaaaaag tgcgccattc
     8401 taacagcctg gcgcaccagc tcgcgccggg cacagcgctt gattagccgc gcgccacatc
     8461 gctgactata aaggccgcca cagtcacaaa gtaagcctgc ccaagcatca tgctcatcag
     8521 tataattttg ttaccaattt ctaacccttc cctccctttc tcaagacgtc cctaaaaatg
     8581 gtcatagatc ccttctcgat cacgggcttg ctcgtgctgg cgatattcct gattggcatg
     8641 acgctgatcg tttttgaagc acaactggag atggataagt tcaagccggc gatgttcatg
     8701 atgtccggtc tgatcgttat cggtgctcac tacgcgtttt acgaccccaa tggctttcag
     8761 ttcttcctgc atgcgcagag cgagatcaag gaagaactgt tcggcctgat cgcctttatg
     8821 gcgtttatgt ggatggtggt cgagctgctc aacgagcgga acgtcttcac cgcactcaat
     8881 ggctacctga tgcgcaaggg gctcggtgca cgcggcatgt tctgggccac tggcgcgctg
     8941 tcggcgctgc tctcgccgtt tctcaacaac atcaccacgg cgatgatctt cggcaagacg
     9001 gtgaagaaca tctcgaccaa tcagcgctat acgcatatct cgctgtgcaa catcgtggtc
     9061 gcctccaact ccggggtgtg gtttctcggc acctcgacca gcctgatggt ggtgctggcc
     9121 ggcaagatca gcatcgccgg cctgctgttg ctgatcccct cggcgttgat cggctggctg
     9181 ttgttcgccg ccacgctgca cttcttctat ctgcgcaaac tcgatggcca gagcctgatc
     9241 caggtggctg accaggccga gtccgcgctc aaacccggcg gcgctggcct ggcgatagtc
     9301 ggtttctttg ccgtatttgg cgcggtgttg tgcaatatcg tgctgaaagt cagcatcgag
     9361 ttcgccatcg gcattggcct gggcatcgtc gctctgtatg cctggctgct gatgcgccgt
     9421 ggcatcgagc tgccctggca ggatcaactg caaaaggtcg agtggaacgc cctgctgttc
     9481 tttatcggca tcatcacttc ggtggcctgc ctcaaccacg tcggctggct gtcgtatatc
     9541 tcacgactgt ttgaagtgat gcaccccacc gccgttaacg tggtgctcgg ggtggcgtcc
     9601 ggggtgatgg acaacgtgcc cgtggaagcg gcggcgctaa tgtcaaaccc gcagctgggg
     9661 ctcgatcagt gggcactgaa tgcactgatg gtggggattg gcggctcgct gacagtggtc
     9721 ggctctgccg ccggggtgat ggcgatgtcg ctggacaaga cttacagctt tgctgtgcac
     9781 ctgaagtttc tgccggccat tctggtcaac ttcttcggct cgctgggtat ctggtacctg
     9841 cagttcgaac tgtttggcct gtactgaaac gaaaacgcca gggcatgccc tggcgttttt
     9901 tgctgaagtt gcggctcggt taacgcgggc agccactaat cccgttcaag gcgcagctcg
     9961 cgcagcacag cgatagaaat cgccacggcg aacagcaacc aaaagtgctc tgaaagcagt
    10021 gcaaacagac tcatattgca gctcctttgc ttgaaatcgc cattcctcgg cagcacctcg
    10081 attctgctcg ccgcataggc agcatgcaag aacatcgacc acaaaggttc acagcagcgc
    10141 atcacaggcg gacaataggt ggactgtcca tgccagcctg gagttcctgc catgtccgcc
    10201 cctaaaacga tgttcgccct cactggccgc agccattcgc ccgccagcct gaacaatgcg
    10261 accttgctgg tcattgatgc gcaagaggaa taccgcagcg gcgtgttgca actaccaggc
    10321 ctggatgccg cgctggaaga aatcgccaaa ctgctctcag cagcccgcgc ccagggcagc
    10381 gccatcgtcc acgtcaaaca cctgggcatc cccgatggtc tgctcgaccc ccgcggcccg
    10441 cgcggcttac acctgccgga agccgcaccg ctgccggggg aaatcatcgt cgaaaaacgc
    10501 atgcccaacg ccttcaacgg caccgatctg catgagcgct tgcaggccct gggccatctg
    10561 gacctgatca tctgcggctt tatgacccat tcgagcatca gcaccacagt gcgcgccacc
    10621 aaggactatg gttaccgctg caccgtagtc gatgccgcct gcgccacacg cgacctgccg
    10681 acgctagacg gccaaataat cagcgctgcc gagatgcacc gcgccgaaat gatcgccctg
    10741 gccgataact ttgccgccgt ggtgccgcgc gccagcgcgc tgatctgaat aaccgccaac
    10801 gcgcagtagt tcgcaacgtg cacaagggct gcactgcgca gttgaaccac accagtccac
    10861 accggtcata ccgccgatag ccaccgagga aattggcatg aggaaatcgg aatgaaacac
    10921 tccgatggtt ttgatgcacg ccgcctacgc ccgcgcaatg ccagcaactg gcgcctgcgt
    10981 ctgggcgctg gcctggcagc gttgttcgcc acatttggcg tgctgctgag tatggcaggc
    11041 accgccgccc tgctcggccg gccggctgca cttggcccgc tgaatgagtc accggctgct
    11101 gccagcattg tggttatcag cggtctgctg ttgctgagtt tcggcgtgat gctctggcgt
    11161 cgctgccgac gtcgcctacg ccgccccagc gacctgagca tggctccgca cttgatgaaa
    11221 aagcgcgact agatccaggc aagcacgaaa gccggcagct tgggtacact agcggccttc
    11281 gcggaggcct aaatgcaaga cgacgatttt tccctattca aagccgagtt gcgcggcgtc
    11341 aaaccgatca agcatgaccg cgccgacacc ggtaaaccga aacccaaccg tgcccaactg
    11401 gccaccctgc gccaggcggc cacggcgcgc gtggagtcag tcaaggtcga cggtctgtcc
    11461 gatcagttcg tgattgatgt cggcgccgaa gacccactct actgggccgc caacggcgtg
    11521 caggaaggcc agatgcgcaa gctcaagctg ggtcagatca gctttgaagg cagcctcgac
    11581 ctgcacggca tgaccttgga aaaagcccgc gacacactgt gggaatttat cgccgaggcc
    11641 aacaagctgg aggtgcgctg cgtgcgcatc acccacggca aagcggtgcg catggatggc
    11701 cgcaagccga tgataaaaag ccacgtcaac acctggctac gccagcatcc acaagtactc
    11761 ggctttacct cctgcctggc caaacacggt ggcaccggtg cggtgtacgt gatgctcaag
    11821 cgcaccatga tggatggccg cgaggaataa gctcctgtag ctggcggcct acgcacttgc
    11881 cagcccgacc ctcgcggcct aacctgcgcc ctttagaaag acctacgaga ttgtccatgt
    11941 ccctggaaca gaactacacc gcgatccttg gccagcttgg cgaggacgtg tcccgcgagg
    12001 gcctgctcga cacgcccaag cgcgccgcca aagccatgca gtacctgtgc aagggctacc
    12061 agcaaaccct cgaagaagtc accaatggcg cgctgttcag ctccgatgcc agcgagatgg
    12121 tgctggtgaa gaacatcgag ctgtattcgc tgtgtgagca ccacctgctg ccatttatcg
    12181 gcaaggctca tgtggcctat atccccaacg gcaaagtgct cggcctgtcg aaggtcgcgc
    12241 gtatcgtcga tatgtatgcg cgccgcttgc agatccagga aaacctcagc cgacagatcg
    12301 ccgaagccat ccagcaagtc accggtgccc ttggtgttgc ggtggtaatc gaagcgcagc
    12361 acatgtgcat gatgatgcgt ggcgtggaga agcagaactc ctccatggtc acctcggtga
    12421 tgctcggcga gttccgcgaa aacgctgcca cacgtagcga gttcctaagc ctggtcaata
    12481 actgatcaag cttctgctac agagaaaccg gccctgtgcc ggttttttct gcccccacac
    12541 ctcaagcagc ctggcgagat tccgccaaaa atcatgtaat tccgccaaaa catggcgaaa
    12601 tcacgccagc caaaaccgcc gttctattct gtgaactgcg ccagagcacc gcagtccaac
    12661 gctttacggt ggcgggcatg cttcttgcca cttgccatta gtcagcccca ggcaacgcca
    12721 gtcgccgcgc cttgggccgc tggcgttatg catacgacgc cctgcacacc ctacaacgcg
    12781 cctgtcgctc gccgacgggc tcgtttgcta ttgccgcacg cctgatcccg tgtcggcaca
    12841 ggtcgtcatt ttgacttcac cgataagatg gttggtttaa tgaaaaagct cctgttgacc
    12901 ctgctgtgcc tgagtgtgat tggctgtgcc aagaaccctg agccggaaaa atccgtggat
    12961 gttctgctga ttggcggcgg catcatgagt gccagcctgg gcacctacct caacgagctg
    13021 gagcccggct ggagcatcga catctacgag cgcctcgacc gtgtcgccga tgaaagctcc
    13081 aacgcctgga acaacgccgg caccggccac tcggccttct gcgagctgaa ctacacccca
    13141 gagctggccg atggcagcat cgatatcagc aaggcggtgg cgatcaacga gtccttcgag
    13201 atttccaaac agttctgggc ctatcaggtt gagcgcaagg tgctgaacaa cccgccttcg
    13261 tttatcaaca acgtgccgca catgagcttt gtctggggcg atgacaacgt cgacttcctc
    13321 aagaagcgcc acgccgccct gcaggccagt tcgctgttcc gcggcatgga atattccgaa
    13381 gacccggagc agatcaagca gtgggtaccg ttggtgatgc aaggccgcga gcctgggcag
    13441 aaactcgccg cgacccgcgt atcgattggc accgacgtca atttcggcga aatcacccgc
    13501 cagctgttcg attcgctaag ccagcaggac aacgtcagcc tcaacctgca gcatgaggtg
    13561 cgcgaccttg agcgcaatgc cgacaactcc tggaacgtac tggtggctga cctggccaac
    13621 ggcggcgcag aaaaaaccat caacgccaag ttcgtgttta tcggtgccgg tggcgccgcg
    13681 ctgaaactgc tgcagaagtc gggcatcagt gaaggcgaag gctacgccgg cttcccggtc
    13741 ggcggctcgt tcctggccac cagcaacccg gaaatcgtcg cccagcatca ggccaaggtg
    13801 tatggcatgg catcggttgg ctcgccgcca atgtccgtgc cgcacttgga tacccgcgtg
    13861 atcgagggca agaaggtgct gctgttcggg ccgttcgcca ccttctccac caaattcctg
    13921 aaaaatggct cgctgctcga caccttcagc gccctgacta ccgacaatat cctgccaatg
    13981 actcatgccg gcatcgataa cttctcgctc agcacctacc tgatgagcca gctgatgctc
    14041 agccaggaag accgcatgga ttcgctgcgc gagtacttcc cggaagcaca gtcggaggat
    14101 tgggagctga tccaggccgg ccagcgcgtg cagatcatca agaaagacgc cgaacacggc
    14161 ggcattctgc agttcggaac cgaagtggtc agcgccgccg atggcagcct cgccgccctg
    14221 ctcggcgcat cgccaggtgc ctcgacggcc gcgccaatca tgctctcggt actggaaaaa
    14281 accttcaagg acaagctgct gagcccggag tggcaaacca agctgaaaga aatcatcccg
    14341 tcctacggcc aaaagctcaa cgacgacctg gcgctgacca accagacccg cgcctggagt
    14401 agcgaacgcc tgcaactgga gttcgttgaa gtgccaccgc tggccctcac cccagaggtc
    14461 agccaggcgc agtaagcgcc tgccggcagt gcaaattgcc aggagcacct tttaccgtcg
    14521 ctttacggcg acataccctg cggccatgga tggcacgcaa taaaaaaccg gccataagcc
    14581 ggttttttat tgcgccgtga aaagcctatt gccccttgaa cggcacccgc ggcttggttc
    14641 gtagcggatc gagcaggccc gacaggccgt tatggtcgat ctcctgcatc agcgccaaca
    14701 gccggccgat ttccccctga gggaagcctt cgcgggcaaa ccagttgagg taattacccg
    14761 gcagatcagc gagcaagcga cctttgtgtt tgccaaaagg catctcgcgg gttaccagca
    14821 acagcaggtc ttcaggtttc atcgcaggct cggcagggtt cggcaatcga gcgtggatta
    14881 tgccgttcag cccagggcca ggccaaggcg gaaaaactcg ggtaagctgc gcgcccctgt
    14941 ttatcgaccc aaggtttgcc tcagcatgat tatcaaggca ctgcgcatcg gcctcggcca
    15001 gttgatcatc ttcctcgact ggatcagccg tccggccaag ctcaaacgta ccccgcaagc
    15061 tcaggctgcg gtcgagcaaa gcgccagcaa cctcgcgctg taccagttcc acgcctgccc
    15121 attttgcgta aaaacccgcc gcaccctgca caagctgaat gtgccggtag ccctgcgtga
    15181 cgcgaagaac gacgcccagg cgcgcagcga gctggagcag caaggcggca agatcaaggt
    15241 gccgtgcctg cgcatcgaag aaaacggcca gagcacctgg ctgtatgagt ccaaggcgat
    15301 catcgcttat ctcgacgagc gcttcgctgc tgcctgatca ggcaatcggg gccgcctagc
    15361 ggccccaaca cctcaaggct gcacagcctg caactcgctc gcgacaaact gactgccctg
    15421 ttgctgcgca acaaaactca ccttgtcgcc caccttgagc ccttcgagct gggcgacgtc
    15481 ctgcacctgg aacaccatgg tcatcggcgg catgccgata ttggtcaacg gcccgtgacg
    15541 cagggtgatt ttctggttgg ctgcatcgat cttgcgtacc tcgccctgac ttgtggccgc
    15601 ggcgctgttg gtggccggcg cggcgctgtg ttgactggca aatacgggac tggccagtac
    15661 gtaggccaag gcgataacac tcagtagatt tttcatggtc aatcctcttg cttggataag
    15721 gggttattcg gccagcacgc gaatacggcc aaccatgccg gcctggtagt ggccggcgat
    15781 caggcaggcg aaatcgaagt cgccggcacg gttaaaggtc cagatgatct cgccactctg
    15841 cccaggcccg acatgggcca tatacggctc gtcatgctgc ataccgggga acttgaccat
    15901 catcgccgcg tgctcatcca gatccgcctg ggtacccagg acgaactcat gcagcaactt
    15961 gccctggttg cggtgcacaa agcggatggt ctcgccctgc ttgacctcaa gttggtcggg
    16021 gctaaagcgc atctggtcgc tcatgcctat ttcgatggtg cggctgacct gctcaaggtc
    16081 accggcaata ccccagggtt tctgctcttt gactacggca gcgctgtgct gcgcagcgtg
    16141 ttgttcgcta tgacccagcg ctgaaccgct aagcagcaag cccgccaggg cgaatgccaa
    16201 ctgcaaataa gctgtctgtt tcatgggtct acctcctcaa tggccagcat ggccgctagg
    16261 tttacgcact tgcacttcga ccggctcgcc agacggctgc ttgagcggca tcgactgccc
    16321 gccggcctcg cttgaacgcg ccggtttggc cagctcccct ttccattcat aggccacagt
    16381 gccggccggg tgtttgaacc agcctgggtc gcggtaatcg ccgggcgctt gatccttacg
    16441 caccttgagc acggtaaaca tgccgcccat ttccaccgag ccgaaggggc cgtcgccggt
    16501 catcatcggt gcggtgttgt ctggcagcga catctgcatt tctgccatat cggccatgcc
    16561 gcgctcgccc atcaccatat agtcagggat cagcttggcg accttgcggg tcatctcgcg
    16621 gtgatcgaca ccgatcagcg tcggtacgtc atggcccatg gcgttcatgg tgtggtggct
    16681 tttgtggcaa tgcagggccc agtcgccttc ctcgtcggcg agaaactcca gctggcgcat
    16741 ctgccccacc gcgatatccg tagtcacctc cggccagcgc gccgccaacg gccgtggccc
    16801 gccatcactg ccggtaattt cgaattcgtg gccgtgaagg tggatcgggt ggttggtcat
    16861 ggtcaggttg ccgacacgca gacgcacctt gtcgttatgt cgcaccacca acgggtcgat
    16921 gccggggaaa atgcggctgt tgaaggtcca caggttgaag tcgagcatgg tcatgatctt
    16981 cggcgtggcc gcccccggct cgatgtcata ggcgttaagc agaaagcaga aatcccgatc
    17041 aacttcgctg atcagcgggt ggtgctcctt ggggtgggtg acccagaaac ccatcatgcc
    17101 catggccatc tgcgtcatct cgtcggcgtg cgggtggtac ataaaggtgc ccgggcggcg
    17161 cgcgacaaat tcgtagacaa aggttttgcc caccggaatc gccggctggg tcagcccgga
    17221 gacgccgtcc atgccattgg gcaggcgctg cccgtgccag tggatgctgg tgtgttcggg
    17281 cagcttgttg gtaacgaaga tgcgcacccg gtcgccctcc accacctcaa tggtcggccc
    17341 cggcgattgg ccgttatagc cccacagatg ggccttgtag cctggcgcca gctcgcgcac
    17401 caccggctct gcgaccaggt ggaactcctt gacgccgttg ttcatccgcc acggcagggt
    17461 ccagccgtta agcgtcacca ccgggttata aggccggccg ttttgcggtt gcagcggcgg
    17521 ctgggtgtcg gcgctgccct gggacaccgc ctccggtatg ccggccaggg atactcggct
    17581 gaccgccgag gtggcgacag ctgcagtaat cgcgctggca ccgagaaaga aatcacgtcg
    17641 tgaaaccatg ggaaatagtc cttgatcagt gcccggcagg ctcgtcggtg gcggccatag
    17701 ccggtgtgct tgatgacaga ctgggcgcgc ccaacagggc catatccaga tcggccttag
    17761 cgatccagaa atcgcgctgc gcctgcagat agccatcgac cgcgaggatt tgtttacgcg
    17821 catcggccag cagctcgaag gtgctgataa acatgccgtt gtactgcagt acattttcct
    17881 cggcgatacg cgcacgcagc ggcagcacct cgtcacggta atgccgggca atgtcatggc
    17941 tggcctggta acccagatag gcctcgcgca cctgcgagcg cgcgttgatt gccgtggcag
    18001 cggcgcgatt gagcatctgc cggtactgcg cctcggcctt ggccaccttg gcgccgctcc
    18061 agtcgaacag cggcaactcg acgctgatct cgtagccgcg ctgcgtcggc tcttcgttgg
    18121 agcggttatt caccacgccc agctccagca cgttgataaa ccgtgtggtc ttgctcagcc
    18181 ccaggttgct tgccagtcgc tcggcgtcca ggcgcacggc atggatatcc agacgctgag
    18241 ccatggccag gcgctcgaca tccggcaact gctcaggctt agccggcaac tcgggcaggc
    18301 gctcgggcaa gcgaaagtcg atctgctcgc cccacagccc gagcaaacgg gtcagctgct
    18361 cgcggctctg cagccgggcc tgttcggcgc ggatcaggtt aagcccggca tcggcataaa
    18421 aggtctgctc ctgggcgcgc tgcaaggtgc tgtagttgcc caccgccgcc agccgccggg
    18481 ccagctcagc cgatgcttca gccgcctgca tcacctgctg catataaacc aggctttcct
    18541 gcgcggccac cgcgcggtac caagccttgc gcgtctggct ggccagttcg aacagcgcca
    18601 ggctggtttg ccgctgcacc tgctcgaagc gctgggcctc catgcccgag gtcagcggca
    18661 tggcgatcag ccgcgccagg ttgatatgca ggccgcgctc gtattccacc tcgctgccct
    18721 tctccatacg accgaaggaa aagcccggat ttgggatgcg cccggcctgc acgcgctcgg
    18781 cttcgctgat gcccaactga tcgaaacttg cctgcaagtc gcggttattc agcaaggcag
    18841 cctgcaccgc ggcgtccagc gtcagcggct cggccagcaa ctcggcgatc cgcgcggcca
    18901 cctggctgcg ctgctccggg gttttcaccc acaccacctg cttgtccagt tgttgcccag
    18961 ccttctctag ctgggtgaaa cctccatcct gactaaagct ggcgcagccg ctcagtagaa
    19021 caaccattgc gcaaatcacg acgcggctcg tcatgctact cgcgttagtg atggccatgg
    19081 tgcgcctcca ttggcttggt cggcggctcg gcgggctgct gcaccggagc ttcctgggta
    19141 tcggactcct gggcatacgg ctcttgggcg taagtgcgcc agccgccgat ccggccgacc
    19201 agatcattgg cctcgcgcca atcgtgcaac ggttgttcac taaagccctg ataggcctta
    19261 agtggcgatt gatagtgcac agccggcgta gtggcctgag cgtccagcgg gtcgggcgat
    19321 tcggcccagc tgccagcagg cagcagccat gccaggcaga taaacgcctg ccggtgggca
    19381 aagggaaaat gcttcatcga atagtctcgc gtgcagcggc gctgcctgca tttcgcaggc
    19441 aaacaccgac cgatagctaa atacgcgtta cgcgggcctg ggcagcaact gcgccaggtg
    19501 cgtcaacgca cggtttggct taggcgagaa acaggcgtgg gggtctttcg ggggtttcag
    19561 cgataaagcc gaaaaactga tccagtcgcg ccaaggacgg ctcgaccgct tgcagcggag
    19621 aattgctggc aatcgcagtg ctcagggcca ccgcaccgac acaaaaagcg cagaggctgc
    19681 acaggctgtt atcggcggca ggcatggact tgctggtgtt gtcactggcg tgatgttcgc
    19741 catgaacaga ggcctcagca gcctgggcca tgaccaccgc gccgggatga tgcgccgcca
    19801 tttgcgtatc aacctgggcc atcgccccgc tctgctgggt taccgcgcat aactgcatac
    19861 tgatggccgc catgctctgc gccgggaggg cgagcattag catccagatc agagcggtac
    19921 gcaaggtttt acgcatggtg cgagtttgtc ttcagcacgg ttgatgggtc ggattatcca
    19981 cccaataggc catttgtcca cacccgagca tgggcaaaag cccctgtagc tggctatgat
    20041 cggagcccat gaccagcacc ccacccttgc gccaaccctg cccacccggc gcctgcgatt
    20101 gcgggcgcga gcagctgttg cagcaagcca acagcgatgt gcgcatcctg ttgctcaccc
    20161 gcagcgaaga aaaacgcctg ctagagcgcc tggaaaacct ggaaagcctg gcggacctgg
    20221 agaagctgca gcgacgtatt tatgagcagc tcggcgtgcg cgtcgccgtc gcccccggct
    20281 ttaacgaagt gcgcagcatg cgcggcatcg taattcagat cgatgaactg ccggggctat
    20341 gtcgcaaaac ccgtcaggcc atccccaccg ccatccgccg cggtttggaa aaacgtccgc
    20401 agatcgccta cgacctgctt aacgcccatg acttgtttcg ggatatttga aattgtggtt
    20461 gggtagcgga tgcgtgtaac cacatgcatc cctctctgcg cgcgccgatt aatgggtcag
    20521 tgagaaccaa caagcggcta tctgccattt cttctcgatg ctcatcacta agcagtcacg
    20581 acgaaatact cttctcgccc gcatccttgc cctcgcggga aagatgctcg atcaccgcat
    20641 tcatctctgc gccgaacagc agcaccgccg aggagatata caggtacagc aggaaaataa
    20701 tgatggcgcc gatgctgccg taagtggcgt cgtagttggc gaagttctgc gcgtagtagc
    20761 caaagcccag agacgcgcct atccatacca ctaccgcgag caccgagccg ggggtgataa
    20821 acctgaactc ctgctgcaca tccggtgcaa cgtagtacac caccgccacc accagcatca
    20881 gcatccccac caccagcggc cagcgcagcc acgtccaaat ctcgataacc agctgttcga
    20941 taccgaaata atgcgccaac caactcagca cctgcggccc caacaccata aagcccgccg
    21001 ccaccagcag ggttgcggca atcaccaccg tgtagatcag cgacaacggc aagcgcttcc
    21061 aggccggccg cccctccttt acgccatagg ccttgttggt tgcatccatc accgaacgca
    21121 ccgccgcaga tgccgtccat agcgccagca ggatacccag agaaaacagc cctatctttt
    21181 gcgtctgcag ttgttcgatg actccatcaa tcagctccat cgccgtctgc ggcatcacca
    21241 atgtggcttg ctgacgcagc cagtcgaaga agggttgcag attcagcacg ctgatcagcg
    21301 cgaccagaaa cagcaggaag ggaaacagcg aaaagaacag ttgaaaggcc aaggccgcgg
    21361 tataggtcgg cagctcgtcc ttgatgaagt cggccacagt gcgcttgatc accgtgccga
    21421 atttgatgcc ctgtaaatct ggaaaaaatc gcataccgcc tccccaaaac cgcctgatcg
    21481 cgcgcggctc aataggattt ggaacgggcc tgacgcccct gagttcagag catatctatt
    21541 cgcctaccgc cctcgccccg ccaggatgcg tgtcgcgccc tgggttttct ggcagtacca
    21601 gaacactcgg ctgaccccac gggtgatcgc cacataggcc agacgcagac tttcatcctg
    21661 catagcctgg tcgtagctgt tgctgaagaa gcccgagtag gcgtacagcg cattgcgcag
    21721 cggatgcggt tcgggcggct gacagtcgtc gaggatgatc gccacttcgg cctgcaggcc
    21781 cttggcgcgg tggatggtgt aggccttgac cggcagtttc ttgtccagct gcgcggtgat
    21841 ggcctgcagc ggttcgttgc gccgactgag cagcaatacc gcgttgcgct cgcggctgtt
    21901 gcgcccggcg gcataggcgc actgctcgct gatctgcttg agcagttccg gcaggcgtgc
    21961 cttgctgtcg aaacgctgga tcaggcgcac gccatgatcg ccagactggg tggccttgag
    22021 cgcctggctg gctttcgcct gtttgtgctg cacgccaccc agcagcgctt cggcgtcacg
    22081 gataatcggt tcgaccgagc ggtagttatt ggccagggtc agcactgcgc tgcgcttggc
    22141 tttgccttta ccgggaaagt gtcggtcgaa gtcgataaac agctccggcg agctgccgcg
    22201 ccagccgtag attgactgcc agtcgtcccc gatggccatc aggctgacgc tgccgccctg
    22261 cccggccagc gtgcgatgca cggcctgcag ccactggacg atttgtggcg agatgtcttg
    22321 aaattcatcg atcagcaaat gaccaaaagg cgccagcagc tgcgccgaaa cgccctgccc
    22381 gccggcgttc agccgcgctg taagctgggc aaaggcgcca ttaaaggtca tcagcccctg
    22441 cgcctgcaga gaacgctcga agtacggcca gaacagcagc agagcttcgc tgaactcacg
    22501 tacagctgcc gggcccgtca gcgcctgcgg ctgcagggcc gcgacagtga tgccgatgct
    22561 ttcggcaaag ccggcctggg cgtaaaacgc ctcatacagc gacgcagcac tgaattcgcc
    22621 tggcagcttg taagcatcca acggcgcctt cggcagacgc ttgctctcag ccggtggcat
    22681 gggcaattgc agcaggctat gcacccgctg gcgaaattcc ggctgctcgg catagcactg
    22741 ctgataggcc tgtttgagca gacgctgctg ggcaggtcgc aggcggccgg cacttagcgg
    22801 gttatctaac tcgcggctcg ccgcctgtgg gtcatccagc tgttcgaacc agcgcgggtt
    22861 atccagcacg ctcttggcca cagtgcccat ggccgagtgg aaggtgcgca cgcattggcg
    22921 cgcctgagtg gcatcgaagg gatagttaaa gaagcccagc accttaagca gctgttcgcg
    22981 cagctcggcg caggaagcat tggtaaagga aatcaccgtc agctgttcgg gcttgatacc
    23041 caggtgacag aacataaatg ccacccgcag caccagcgtg gtggacttgc ccgacccagc
    23101 cccggcgaag atgcgcgtca gcgggtgatt gatgagaatc atcgcccact gctcatccga
    23161 tggcgcgcta agcaagccgg cagtcaccgc ccgtgccacc tgctcgcgca tggcctgact
    23221 ctgcgtagca tcgaccgcca gcatggctgg tccgtaaatg ccttggatac gcggtttact
    23281 cgcagcgcgg cgcggtttgc tctgcggttt cgcactatcc agtttggcct tggccgccgc
    23341 ttttttggcc cgtggtgcag gcttttttag cggtgcagcc gcttcacgct cggcgcgcag
    23401 ataggcagtg gtatggggga agtaacgcgc cagagcagcg gacaggagcc gcttgtagcg
    23461 ctgaacagca gatggcatcc gcgacccagt caaatagata gatcgttgaa tgataagtgc
    23521 tttttacggc gagctggcga ctgagcggtg agaaagcgca gcgcctctca ccgctcagtt
    23581 cacccggcgg ctgtcagtcg agcatgccga tatagcgcgg gtggctggca acccgcgcca
    23641 gccaggtgcg gatggccgaa tacgaggtca ggtcaaagcc tccttcgtcc gccacatggg
    23701 tgtaggcgta cagcgcgata tcggcaatcg agaagttctc cccaaccaga tacggcgtgc
    23761 gctgcagctg cttctccatc accttgagcg ctttgtgccc gcgtacatgg cactcctcgt
    23821 attctgtgcg gcgcgcttcg ggcaggccct gatagagctg aataaaacgc gccaccgcaa
    23881 catagggctc atggctgtat tgctcgaaaa actgccactg cagcacctga gtgcgcagac
    23941 gcggctcggt tggcagaaac gcgctaccat cggcgaggaa gttgaggatg gcattggact
    24001 cccacagaca ggtgccgtct tccagctcca gcaccgggat cttgccgtta ggattcttgg
    24061 ccagaaaggc ttcgctctgg gtctcgccct tgaggatatc gatcggtatc cactcatagg
    24121 gctgatccag caggtgcagc atcagtttga ccttgtagca gttgcccgag cggtaatcgc
    24181 cataaatctt gtacatgctg cctcctatat cgcctgctct acacgctgca tattgctttt
    24241 gtaggagcta gcttgctggc gatcagcggt tagcaaagca tcgccagcaa gctggctcct
    24301 acacaaccga cgctgcgtcg gcttgtgcct gccgaatcac ttcggccaga cgtttgaggc
    24361 cttcgttcag gcgctccggc gcaacgtggc tgaagttaag ccgcaaatgg ccagggttgg
    24421 cgtccggatc gataaagaac ggctcgccgg gcataaacgc cacgttctgc gccagcgccg
    24481 gcgccagcag cgtgcgcgta tccagcggct gcttgagggt cagccagaag aacaaaccgc
    24541 cttgcggcac ctgccagtca gccaggtcgc taaagtgctc ggccagcacg tcctgcatgg
    24601 catcgcggcg cacccggtag aaatcgcgca gctcggccag gtgttcgcgg taatgccgcg
    24661 tgcccagcca ttgcagcgcc tgccactggc cgatgcggtt ggtgtgcagg tcggcagact
    24721 gtttgaggcg gagcaagtag gggtacaaat ccggagtggc gatcaggtag ccgacgcgca
    24781 gacccggcag cagggttttc gacacagtgc cggtgtagat ccagctggcc ttctgcagac
    24841 ggctgacaat cggcgtggca ctgccctcat cgaacaccag ctcgcgatag ggctcgtcct
    24901 cgatcagggt cacgccaaac tcgtccagca gcgctgctac cgcatcgcgc ttggcttcgc
    24961 tgtaacgcac cgccgagggg ttctggaagg tcgggatcag gtaggcgaag gccggtttgt
    25021 ggttttccag gcgctggcgc agggcgtcga gctgcgggcc atcagcctcc tgcggtacgg
    25081 cgatgcaatc ggcaccaaac agctgaaacg cctgcagcgc cgccagatag gtcggcgctt
    25141 caagcagcac ttcggtaccc ggatcgataa acagcttgct ggccaaatcc agcgtctgct
    25201 gcgaaccgct gacgatcagc acctggctgg cgtcgcaagg tacgcccagg gcacgcgcct
    25261 cttcggcgat ggcttcacgc aactccggct cgccttcgct ggtgccgtac tgacccatgc
    25321 tggtcggcat gtcggcccac tcgaccttcg gcaacatcgg ctcggcaggc agaccgccgg
    25381 cgaaggacat cacttccgga cgctgcgcgg cagccaggat ttcacggatc agggagcttt
    25441 tcaggcgggc aacacgttcg gagaaggcca tggatacacc ggtagcaaag gcaaataaaa
    25501 ataggtcaaa ctgtttgacc gaaactctgg ttgacagaaa ctacgacgac caccgcagat
    25561 acgtcaatag gcttgacctt aatgcttgat ttcaagagca ccgccacgca gcaaaccgcc
    25621 atggaagcct tcttcttcgg ctaccaggcg tttaccgcca aagccgatga aatgctcgcg
    25681 cgccgtggcc tgtcacgcgt gcatcaccgc atcctgttct ttatcgccaa gtaccccggc
    25741 ttgagcatga aagagctgct cggctacctg ggcgtaagca aacaggcgtt gaataccccc
    25801 ctgcgccagc tgctggaaat gaacctggtg caaagccttg ccgccgaaga cgacaagcgc
    25861 aaacgcctgc tcggctttac cgccgaaggg gccaagctgg aacaggcgct gcgccgcgaa
    25921 caggccaagc tgttgcaacg agcgttcagt gaagccgggg aaacggccgt cagcggctgg
    25981 ttgcaagtca accaaaccct gcacaacagc gcaaacgaag gctgacagag cgcggctggc
    26041 aaactgtacc gaccacgcca gaccactgtt gaactgtacc gctcattgcc tgcctcgctg
    26101 taccgcgacg ccctgcccgt ctgcccctag gctgatcagc actgctcccc caatgagtgc
    26161 tatgaggctg cgccatgacc accgaactgc tgctcgcctt tatcgccttt gcctttgtca
    26221 cctcggtgac gccggggccg aacaatatga tgctgctggc cagcggagtg aacttcggcc
    26281 tgcgccgcag cctgccgcat atgttcggca tcagcctggg ctttatgctg ctggtagctt
    26341 cggtcggcct cggtttgggt caactgttcg aacaggtacc gctgctctat agcgtgttgc
    26401 gctacctcgg tgccgcctac ctgctgtacc tggcctggaa aatcgccaac tctggcgcac
    26461 cggacagcca gagcaacgcc gccggcaagc cgttcagttt tctgcaggct gcggccttcc
    26521 aatgggtcaa ccccaaagcc tggatcatgg ccatcggtgc catcaccacc tacacgccgc
    26581 aggaaaactt cgtggtcaat gtgctgctga tcgccgcgct gttcgccctg gtcaactgcc
    26641 ctagcgtcgg cctgtggacg gtggctggca gcctgctgcg caactggctg aacaatgccc
    26701 gcgcactgcg cgtgttcaat atcggtatgg ccctgctgct ggtagcctcg ctgtacccca
    26761 tcttcgccga cactggattg ctctgatgca cgacctgccg caagcccgcc tacggccgct
    26821 ggccgacacc tcaacctcgg cagtggtcgc cgggtttatc gccatgctta ccggctacac
    26881 cagctcgctg gtgctgatgt ttcaggccgg ccaggctgca ggtctaacta acgggcagat
    26941 ttcctcgtgg atctgggcgc tgtcgattgg catggcgatc tgctgcatcg gcctgtcact
    27001 gcgctaccgc gcaccgatca tgatcgcctg gtccaccccc ggcgcagccc tgctgatcac
    27061 cagcttgccc ggcgtgccct acagcgaggc gattggcgcc tatatcttcg cctcggcgct
    27121 gattgtactg attggcttga ccgggacctt cgaccgcctg atgcgccgca tccccgcctc
    27181 catcgctgcc gcgctgctgg ctggcgtgct gttcaagatt ggcctggaaa tctgcgtggc
    27241 tgccgagcag cagccggtgc tggtggtggc gatgctgctc gcttacttgt ttggtaaacg
    27301 actgctgcca cgctatgcgg tgctggcggc actgattgtc ggcactgtgc tggccgcgat
    27361 cttcggcctg ctcaacttcg agcacttcga gctgcaaatg gcggtgccgg aatggaccac
    27421 gccaagcttc tccctggcag cggtcatcag catcggcatt ccactattta tcgttgccat
    27481 ggcctcgcag aacttgccgg gcatggctgt gctgcgcgcc aatggttatg acgtaccggc
    27541 cagcccgctg ctcaccacca ccgggctgac ctccatgctg ctggcgccct ttggcagcca
    27601 tggcatccat atggccgcca tcagcgcagc gatctgcgcc ggcccggaag cccacgaaga
    27661 cccgaaaaaa cgctacaccg ccgccatctg gtgcggggtg ttctacggca ttgccggtat
    27721 cttcggcgca acccttgccg cgctgtttgc cgctctacca aaagccctga tcctgtccat
    27781 cgctgccctt gcgctgttcg cctcgatcat cggcggcctg acccaagcca tgagcgagcc
    27841 gaaagagcgc gaagccgcgc taatcacctt tctggtaacc gcctcaggca tgaccctgtt
    27901 ctcggtgggc tcggcatttt gggggattgt cgcgggtttg ctgaccttgg cgattcttaa
    27961 ctggggcaag caggacgctt gagtgatggg tattgctgaa ctcgaagctc cgtgggaggg
    28021 gctttagccg cgacaaccgc tataaaagct cgccgctaaa gcgcctccta catgtcatat
    28081 atcacccaca ataaaaaact ggcttatgca tacgcataag cccg
//
LOCUS       DASRUM010000220        42362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1630309, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000220 DASRUM010000000
VERSION     DASRUM010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 42362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42362
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630309"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFF22_14880"
     CDS             <1..257
                     /locus_tag="VFF22_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZX18133.1"
                     /translation="VLLAMGLDPVWLGVMIALNLQTSFLTPPFGFALFYLRGVTPASV
                     PTSTIYKGVVPFILIQILLLVIAYQFPGLITWLPEQVYGK"
     gene            363..1268
                     /locus_tag="VFF22_14885"
     CDS             363..1268
                     /locus_tag="VFF22_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HZX18134.1"
                     /translation="MARSHVERLELDELTCWRARVGTRELLISQQGAQILSYQEDDQP
                     PLIWLSEQAAYKRGQGVRGGVPVCWPWFGGLQRNPQAVQAMHLQPAAAPAHGLVRNLD
                     WQLQGIDSRDDGVQLEFVYNSATQPLTDWPHAAELRLSIRLDERLHIQLSSRNLGDTP
                     LAISQALHSYFAVSDIRNVAVEGLHGCRYIETLEDWQQRQQDGLLQFSGETDRIYQGT
                     PAQLSILDRAWQRRIQLRSSGSSSAVLWNPWIDKAQRLSQFADDAWTGMLCIEHANVL
                     DDTRVLAAGEQHQLDVSLWSEPCSA"
     gene            complement(1738..2172)
                     /locus_tag="VFF22_14890"
     CDS             complement(1738..2172)
                     /locus_tag="VFF22_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008073506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZX18135.1"
                     /translation="MRDLEVEQPDLAEKIINIIESGNVMADEGAHEKALADYNQAWDL
                     LPNPKIEWTMISSWLVGCFYESFFQLEDFKSALHWASIELDIRESEIDVGPWMNLGKV
                     YYELNDHDAAFNYFKKAFDFGKARVFKEEPKKYYEFFKSKNI"
     gene            complement(2182..6630)
                     /locus_tag="VFF22_14895"
     CDS             complement(2182..6630)
                     /locus_tag="VFF22_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017135815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HZX18136.1"
                     /translation="MSDALWAARLGDDLLHTTLLADIVGGVLEVAAYAAITAVACAAV
                     VAATGLTVLTGGLGGFALAAVVGIAVGVGMNKTGWDKGLTTLCEGLGNAISPPNVQAK
                     IVTGANKTKTNGLPAARAAGKLGSAAAPSGTELEQAPPAEEPEPSFLDMAKSFFSEMW
                     QPTVAQPAPGVIPCPLDAIECKKHPPMPVQLLAEGSIKVSIEGQPAVRSGDRSTCEAT
                     VVESGNISPNVRIGGDAVVVQPIRSGKTPGIGLALAVLMALRGNPRKLCSKLPCLAAG
                     FAGNMAMGGATAYATNSIVNALGSASSGAPNPVHASTGAKVLGEDDDLDFVLPGLMPI
                     EWQRFYSSCDERRDGLLGAGWSLPFEIAVQVRAHPEGGEALTYIDEQGRPIEIGSLPP
                     GDACYSPGEGLNFRRDESGALLVESVDGLYRLFEPLPESPERLRLSKLGDRNDNRLLL
                     EYDATGRLRAVRDGHNQLCIELIADAQHPQRLGRVERLFADERREVLVAYAYDTQGDL
                     SEVRDDKGQLLRRFVYDGGHRLIEHQRPTGLRCFYQWSEFQGADGREWRVTRHWTDDG
                     DEYHLHYDLDAGVTRVTDGLGRISERHWNNQYQITRYRDALGQLWLFEWNDERQLLAA
                     VAPDGARWQFAYDESGNLCETLDPLGRRDFTQWLGHWSLPEAETDAAGNAWQYRYDKR
                     GNCIAEIDPLGHVTRYRYDTSGLPVEIIDASGKRKTLRWNALGQLTEQVDCSGYPTRF
                     AYDEHGNLVQITDALGEITRYHYDSQGRLLQTQLADGRSEEFQRDAIGQLSAYLDPAG
                     KPVRYRYDRRGQVRQRIDALGRRVGFEYDAYGRLLALSNENDERYRFTWDVLDRLSAQ
                     HDLDDSARHYRYDPQGNLIGLAYTPAPHGTGLGSVPEAPPVPIIHRFERDAVGRMIAK
                     HTDDGRTEYAYDPLDRLLSIDLTAVDGQVQSLAFAYDALGQLLSEQSAAGALQHAYDE
                     LGNLLETQLPDQRRIRRLYYGSGHLQLLSLDDQLVSRFERDRLHREVLRTQGQLVTRS
                     QYDRSGRLTARLRRRLEQPVQLPAEAERRYAYDPSDNLIGRLDRDPASQREQKQQLHY
                     DASGRIIASQDLIRGNLETYSYDAAANLLQPVGGDAASSGWVRHNKLLTYQDKRYRYD
                     AFGRLVEKRSGRHQLQRLSYDAEHRVREVTNNDGSRVVMHYDPLGRRIGKSHYDAHGV
                     LLGQTDFTWDGLRLLSETRNGRHSLYLYDEGSYDPLARVDGQGEHARLRYYHTDPNGL
                     PQQLTEDDGRCIWQARYQVWGNTLAEHQESFFVEEQNLRFQGQYLDRETGLHYNTFRF
                     YDPDIGRFISPDPIGLAGGINLFQYAPEPYGWVDPLGWSCRLSYVGRTPSKNSKTGKA
                     VIARMRTEGRIRGTGANMKFKSSDGKWYNVKHADMAHKTDAVSYWNKKGGFHGAKSKE
                     VRAWMRDPDNYELEYFSHNRSKGASLGETYKDPGGFIGPAEKSTYF"
     gene            complement(6637..7065)
                     /locus_tag="VFF22_14900"
     CDS             complement(6637..7065)
                     /locus_tag="VFF22_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HZX18137.1"
                     /translation="MSYTTQELSLQLPGKDLQDSTINILRLNDLGTSLVITRGALGDG
                     ETLRSHFEAQVAKLAKQVKDLRCSALQDVQVGPAQAIVGVELRSQFSKGPEQVYQFQI
                     GVLLADGRRLLALSYVKPTPLDDADAAHWAQIKHSLSLNG"
     gene            complement(7065..9005)
                     /locus_tag="VFF22_14905"
     CDS             complement(7065..9005)
                     /locus_tag="VFF22_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HZX18138.1"
                     /translation="MLFAQSSRFARITSPLGPDVLLLRSMHGSDELGRLFEYELQLVS
                     HDANLDLNQLLGKPLTLSVQQHGGGERYFHGIVAACGQTVDRGQFAAYRVTLRPWLWL
                     LSRTSDCRIFQHMSVPQIIKQVFRDLGFSDFEDALSRPYREWEYCVQYRETSFDFVSR
                     LMEKEGIYYYFRHEADRHVLVLADAYGAHVPAPGYASVPYYPPDGQHRERDHINDWQL
                     AQHVQPGSMELNDYDFQRPSARIDVRSVMPRPHSAGDYPLYDYPGTYVQSSDGEQYAR
                     NRIEALQSLHERVEVGGSVRGIASGNLFSLTGFGRADQNREYLVVCARYHVAQESLES
                     GSGGGGQFECQLTCTDAQQTFRPLCGTPKPMVQGPQTAVVVGPAGEEIWTDQYGRVKV
                     HFHWDRHDQSNENSSCWIRVSQSWAGKNWGHIQIPRIGQEVIVSFLEGDPDQPIITGR
                     VYNAEQTVPYDLPANATQSGIKSRSSKGGTPANFNEIRMEDKKGAEQLYIHAERNQDI
                     VVEVDETHSVGNNRVKSIGMNETVTIGNNRLRAVKLDDVLLVGSTKTDSISQSYLIEV
                     GQNLRLVCGKSILELNASGQINLSGVAINFHASGDAEFNTGGVLNLNISGGPGATPDG
                     QGDQGSIDAAVKSLFGQEKGNS"
     gene            complement(9064..11736)
                     /gene="tssH"
                     /locus_tag="VFF22_14910"
     CDS             complement(9064..11736)
                     /gene="tssH"
                     /locus_tag="VFF22_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003177222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HZX18139.1"
                     /translation="MGEISRTALFGKLNSVGYKAIEAATVFCKLRGNPYVELAHWFHQ
                     LLQLQDSDLRRIIRQFSLEPARLARDLTDALDRLPRGSTSITDLSSHVEEAVERGWVY
                     GSLMFGEHQVRTGYLIIGILKTPSLRQVLLGLSGEFAKIKVETLSERFDEYVGDSPEN
                     NLAASDGFAAAAPGEASGAMAPAAMGKQEALKRFTLDLTEQARKGELDPIVGRDEEIR
                     QLVDILMRRRQNNPILTGEAGVGKTAVVEGFALRIAAGDVPPALKDVELRSLDVGLLQ
                     AGASMKGEFEQRLRQVIEDVQASPKPIILFIDEAHTLVGAGGAAGTGDAANLLKPALA
                     RGSLRTVAATTWAEYKKHIEKDPALTRRFQVVQVPEPSEDKALLMMRGVASTMEKHHQ
                     VQILDEALEAAVKLSHRYIPARQLPDKSVSLLDTACARVAISLHAVPAEVDDSRRRIE
                     ALETELQIIAREHAIGIDVSLRQANSESLLAAERERLAELESRWAEEKQLVDELLATR
                     ARLRESVGMVDQDSDSDHQGLRAQLVELQQRLSQLQGETPLILPTVDYQAVASVVADW
                     TGIPVGRMARNEVETVLNLDSLLAKRIIGQDHALKMIAKRIQTSRAGLDNPSKPIGVF
                     MLAGTSGVGKTETALALAEALYGGEQNLITINMSEFQEAHTVSTLKGAPPGYVGYGEG
                     GVLTEAVRRRPYSVVLLDEVEKAHPDVHEIFFQVFDKGVMEDGEGRLIDFKNTLILLT
                     TNAGTEQVARLCQDPDNLPDPEGIARDLRQPLLEIFPPALLGRLVTIPYYPLSDTMLK
                     AITRLQLGRIKKRIESTHKVPFDYDEAVVDLVVSRCTESESGGRQIDAILTNGMLPDM
                     SREFLTRMMEGKPLAGVRISVADNDLNYSFADNA"
     gene            complement(11729..12781)
                     /gene="tssG"
                     /locus_tag="VFF22_14915"
     CDS             complement(11729..12781)
                     /gene="tssG"
                     /locus_tag="VFF22_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HZX18140.1"
                     /translation="MESPARPSTEPLDTLVQMQAQPWEYDFFQALRRIENEHPHLPRL
                     GRSRRLADDPLRLGQRQEGTFAPATLAGVTPASGDRPARLEQYFFGLGGPNGPLPLHL
                     TEYVRERQRNHADSTSKHFLDLFHHRLLTLFYRAWAEARPTVSHDRPDDDYWSARLAA
                     FSGRGMPSLRQRGLLDDAAKLHFSGHLAAQTRYPDGLKAILADYFGVPVQIEEYAGSW
                     LRLPERTRLGVESSQLGVDFCLGSQVWDRQHNFRICFGPLTLEQYNAMLPDGESFAAL
                     VAWVAEYQGDELDWQLNLVLQREEVPALCLNGQARLGYTTWLGQPQHDARDLVLARHH
                     AAATPPADQSRRAPHG"
     gene            complement(12745..14604)
                     /gene="tssF"
                     /locus_tag="VFF22_14920"
     CDS             complement(12745..14604)
                     /gene="tssF"
                     /locus_tag="VFF22_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HZX18141.1"
                     /translation="MNPRLLELYNQELLHIRESAQEFAREYPKIAGRLALSGMECADP
                     YVERLLEGFAYLTARVQLKLDAEYPTFTHNLLEVAYPHYLAPTPSMTVVQLHTDANDG
                     ALNGSFNLPRDSVLRATLGKDSQTPCEYRTAHPVTLWPLQVADAEYISNPAAALGRLA
                     ASEPRAKAALRITLRSGAEIPFSSLDLDNLPLYLSGLDELPFRLYEQLLGNRIAVFAR
                     QPGRDWVERLPADALRPCGFDDAEAALPVVPQAFQGYRLLQEYFALPQRFLFVDFCGL
                     RRAVRQCSGEELELIVLFDRLDSHLQNQVGASQFLPFCTPAINLFPRRLDRIHLSDKV
                     HEHHAIADRTRPMDFEIHSLRGVTGHGSGPEQPFLPFYAVRDPARYGRDQAYYSVRRE
                     PRVLSAEQRRSGTRSSYVGSETFISLVDSHQAPYRHDLRQLGLSALCTNRDLPLFMTV
                     GIGKSDFTLADSAPVQSVRCVAGPSRPRASPAHDNQAWRLISQLSLNYLSLAEQGQGA
                     AALREMLRLYGDMQNPALQLQIEGLREVSAKPCTRRLPMPGPIVFGRGLEITLEFDEN
                     AFRGTGVFLLGAVFERFLARYVSLNSFTETVLRSTERGEIMRWKARPGRRPNL"
     gene            complement(14601..15116)
                     /gene="tssE"
                     /locus_tag="VFF22_14925"
     CDS             complement(14601..15116)
                     /gene="tssE"
                     /locus_tag="VFF22_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008007710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HZX18142.1"
                     /translation="MVTSMTPRERLQPSLLDRLTDDDPGNPQESADRRVLSMNQLKAS
                     VLRDLTWLFNTIALLNADTTRHTPAGTSVINYGLPPLAGSSVSGIDIAALENLIQRAI
                     ITFEPRILAHSVRVRAHVGHEQMNHNALSFEIQGDLWAQPVALPLLLRTELDLESGHV
                     QVLPAEQRRRS"
     gene            complement(15110..15547)
                     /locus_tag="VFF22_14930"
     CDS             complement(15110..15547)
                     /locus_tag="VFF22_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007252915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18143.1"
                     /translation="MRILPLNALLATLLVSLSACAEHGASPSVGITQAEFAGQSVRLS
                     DAQGRCALALADAAPLTLDMQWPCRFSETPEKQLRVEYFRQTPIIMVERSEPQPAPSR
                     SCLTDLQALRLHQGQLEAAPVSRIAACGPSQWDQKAFVGMFDW"
     gene            complement(15547..16002)
                     /locus_tag="VFF22_14935"
     CDS             complement(15547..16002)
                     /locus_tag="VFF22_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007894486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system amidase effector
                     protein Tae4"
                     /protein_id="HZX18144.1"
                     /translation="MSKPSFINLWKAYEDLLVEYPDRKPCDGNWANQCAIRLSLALNN
                     ERTLQINSSTYSEPRCAHGHARGAESLANWLWKRPQLGAPRIYSNSAADRTSLLGKTG
                     IIFYKDFYYQPTDPEGRPSGDHIDLWNRGLTLTGDYFHRAKAVWFWELS"
     gene            complement(16108..16596)
                     /locus_tag="VFF22_14940"
     CDS             complement(16108..16596)
                     /locus_tag="VFF22_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018927416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HZX18145.1"
                     /translation="MAVDIFIKIGDIKGESQDKTHKDEIEVLNWSWGMSQSGNMHTGT
                     GGGAGKVSIQDMSLTKFVDKATPNLMMHCSSGKHVPKVTLTVRKAGGDSQVEYLIINL
                     EEVLISSLSTGGSGNDDRLIENITLNFAKVTVDYQPQKADGTKEGGPIKYGWNIRSNV
                     KI"
     gene            complement(16720..18213)
                     /gene="tssC"
                     /locus_tag="VFF22_14945"
     CDS             complement(16720..18213)
                     /gene="tssC"
                     /locus_tag="VFF22_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007894498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HZX18146.1"
                     /translation="MSEQLQQDQAALASTEQTSEFASLLMQEFKPKTERAKEAVETAV
                     RTLAEQALAQTDLISNDAIKSIESIIAAIDAKLTAQVNQIIHHSDFQQVESAWRGLHY
                     LVNNTESDEQLKIRVLNIAKPELHKTLKKFKGTAWDQSPIFKKMYEEEYGQFGGEPYG
                     CLVGDYYFDQSPPDVELLGELSKVCAAMHAPFIAAASPTVMGMGSWQELSNPRDLTKI
                     FTTPEYAGWRSLRESEDSRYIGLTMPRFLARLPYGAKTDPVEAFAFEENTDGADSSKY
                     TWANAAYAMAVNINRSFKHYGWCSRIRGVESGGEVPNLPAHTFPTDDGGVDMKCPTEI
                     AISDRREAELAKNGFMPLLHKKNTDFAAFIGAQSLQKPAEYDDPDATANANLAARLPY
                     LFATCRFAHYLKCIVRDKIGSFKERDEMQLWLQDWILKYVDGDPAHSTETTKAQHPLA
                     AAEVVVEEVEGNPGYYNSKFYLRPHYQLEGLTVSLRLVSKLPSAKGA"
     gene            complement(18228..18743)
                     /gene="tssB"
                     /locus_tag="VFF22_14950"
     CDS             complement(18228..18743)
                     /gene="tssB"
                     /locus_tag="VFF22_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007894500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HZX18147.1"
                     /translation="MANSSSQKFIARNRAPRVQIEYDVELYGAEKKVQLPFVMGVMSD
                     LTGKPAEPLAPVAERKFLEVDVDNFDSRLKAMKPRVAFNVPNELTGEGNLSIDMTFQS
                     MDDFSPAAVARQVDALKQLLEARTQLANLLTYMDGKTGAEDIIMKAIKDPALLQALAS
                     APKPQDNEPQA"
     gene            complement(18829..19854)
                     /gene="tssA"
                     /locus_tag="VFF22_14955"
     CDS             complement(18829..19854)
                     /gene="tssA"
                     /locus_tag="VFF22_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016975952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HZX18148.1"
                     /translation="METSLLLTAISDASPCGEDLEYDSDFLQLERDALGRPERSMGDA
                     IQAAEPPDWRQLEQACHLLLLRSKDLRITRYLLQSRLALRGVEGLADSLELILELLSR
                     YWPEIHPQLDADDDNDPTVRINTLSALTCETNLGLLRSAVLVRSRVFGNITLRAVLNA
                     AGLHLASSESLSIEELDGALQDADSEALQLTHEALKRALHSLNAIEQLVSEQVGSAQG
                     VDFSALQQPLKLALQVLGDSPAGASSEDYPAGGEAAQEQLAQHASGSGVARVSGEISN
                     RDDVLRSLERILTYYARHEPSSPLPVLLNRARSLVNADFATIVRNLIPDGMSQFENLR
                     GPDADQA"
     gene            20247..21656
                     /gene="tagH"
                     /locus_tag="VFF22_14960"
     CDS             20247..21656
                     /gene="tagH"
                     /locus_tag="VFF22_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HZX18149.1"
                     /translation="MALRLTITSYHKITPGQCPEKTLEHGVMAIGRSPDNDWVLPDPA
                     RLVSSKHCLVQNKDGRYYITDSSTNGVELVKAGVRMQRGNSELLNHGEVIRIGKYEIQ
                     VALESSIAEGSAAFSAAPSGSSFEALMAGHQDAPAVPFSPPANPFDSSASAHLQSVSP
                     HETLPDLFDFLDPSTSAPKPIADHVPAQQHDFRPPEVSPPPVEQKPSDEPGLIPDDWD
                     LLDDITPAATTPAASLPVDLSLPPEPTEPAPLPPLPAVPDPTPPPATSQPQLAQDDDV
                     LLQAFLRGAGLERLRLDTQRAAAQMESIGRSYRLMVDGLIDVLRARSSLKGEFRLQQT
                     MVQPVENNPLKFAPNAEEALLLLLRGGNSAFMSADLAVQDSFDDLRAHQLAVMAGVQA
                     AIRHLLARFEPQALEARMGKGGGLASLLPNARQAQCWQQFVELYATLSREAEDDFQDL
                     FGREFGRAYEQHIARQRRP"
     gene            21680..22195
                     /gene="tssJ"
                     /locus_tag="VFF22_14965"
     CDS             21680..22195
                     /gene="tssJ"
                     /locus_tag="VFF22_14965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HZX18150.1"
                     /translation="MRKRTLRPVLLGLLLAALNSGCSKDAMDNADTAQGPTTVVLQLQ
                     AGDELNPTTDGHSAPVRVRLLELRSAAAFSRTDYFSLTERAAASLGNELVAEDEWLVH
                     PGQARELTRVLNPATRHLGLLVGYREIDRAQWRLVLEPKQGMLSHYRIDLGINAASAA
                     QLAAPSRLDSR"
     gene            22208..23542
                     /gene="tssK"
                     /locus_tag="VFF22_14970"
     CDS             22208..23542
                     /gene="tssK"
                     /locus_tag="VFF22_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HZX18151.1"
                     /translation="MSWNNRVIWSEGMFIGTQHFQQHDRYLEALIDARSRPLSAAGWG
                     FADLQLDMGLLAQGKLAIVSARGLLPDGTPFNIPQDDVAPEPLEIPEDLRDGVIYLAL
                     PLRRAGVRDTVEAGEALSGARYRGQVREVRDDNAAFESRVPVMVGERALRLLSAADGL
                     QEYAALGVARVREKRADRALVLDEQYIAPLLDVAASAPLAAFRGELLGLLHQRGEALA
                     GRVVASSAGGASEIADFMLLQLVNRAQPLFAHLAQLQPLHPERLYSELLALAGEFATF
                     SSSGRRPSEFPTYLHDDLQTSFTPVMQALREALSMLIDSKAVPIPIVEKAYGVHVAML
                     ADRSLLDNASFVLVVRADMPAETLRGRFGQQSKIGSVEHIRNLVNLQLPGINLLPLPV
                     APRQIPFHAGSTYYELDRGSEHWKQLAHSGGFAFHVAGEYPGLNLAFWAIRG"
     gene            23546..24877
                     /locus_tag="VFF22_14975"
     CDS             23546..24877
                     /locus_tag="VFF22_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type VI secretion system protein"
                     /protein_id="HZX18152.1"
                     /translation="MTDQDDWLNKPPAAQPQNNDRTQFMPRPGGRGPQPPVEAPAAPL
                     QMPAAPLPAGQARGMNPLEQAAGPLLSMLTRLRSTLAHPAPASLRAQLLGYLRQFEQR
                     AEAAGVPRNDVLLARYALCTALDEAVLSTPWGSTSEWGKQSLLITVHNEAWGGEKVFQ
                     LLEHCLQSPRERLDLLELLYLCTSLGFEGRYRVMHDGRSQLEALRERTAATLHSVRGE
                     REHELSPHWRGVSVAPDRLSRLLPPWVGLAVALALLLVLLLVLRMKLAADAEPVFRQI
                     HALGEVPVQSIEPAVVKPRVIERPRLATFLADDIRANRVAVEDGVDRSVVTIRGDELF
                     SSGSASIRDDFQPLLLRIAEAVAKVKGQVRITGHSDNQPIATLRFPSNWALSRTRAEE
                     VLQILAARTGPAERFSAEGLSDTEPLASNATAEGRAKNRRVEITVLAEGVE"
     gene            24874..28374
                     /gene="tssM"
                     /locus_tag="VFF22_14980"
     CDS             24874..28374
                     /gene="tssM"
                     /locus_tag="VFF22_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003176940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HZX18153.1"
                     /translation="MKALFGFITRWVIPVLGLIALSLIIWFIGPLLNVLQPAWTRWTL
                     IIVLFALWLGWRLFCLLRERQQERKVLDSLAAQTPPDAASVATAEELATLRQRMDEAL
                     VLLKKARLGGDERRNLYQLPWYVIIGPPGSGKTTALVNSGLNFPLAAQLGAGAIRGVG
                     GTRNCDWWFTDEAVLLDTAGRYTTQDSHAQVDKAAWLGFLELLKNQRARRPIDGAFIA
                     ISLSDLLQSSDSERAAHVAAIRSRVQELYSQLGVRFPVYVMLTKCDLVPGFMEFFDDL
                     SREERAQVWGFTFALDNGKSIGGPLESFDSEFVALEQRLNQRLIERLQRERDPARRDL
                     IYGFPQQFAAIKSLLGEFLNGVFKPNAYEERPLLRGLYFTSGTQEGSPIDRLIGAMAQ
                     SMNLDRQHLARQSGSGRSYFLQHLFSRVAFAERGLVGVDQKVKRRRRWLAIGSLAASV
                     ALVLVVGTLWLLSFNANREHIAQVGARTAPLEQQVRSLSPAQRDVLAVLPLLSAVRNL
                     AGSPPDWAKGLGLYQGDMLEEQAASVYRRLLVATFAPRLVSRIEEQLRTGGGSDYLYE
                     GLKAYLMFGGGEQYDAEFITAWIALDWDQSLPRDLSGEQRQALDEHLQALFERRPPDA
                     RLDQRLIDELRMQLQRLPLAQRVYDRVKRQKLPAGVSDFRLSEAAGRDAALVFTRKSG
                     KALSDPLSGFYTRRGYREAFLRASVGQASVVAEEQWVLGSQLDSTQDAVNLAADVRQL
                     YLRDYVQQWDALLADLDFVPITSVAQAADVLRILSGPSSPLKQLLIAVAQETDLQQAP
                     ESEGVAKATDEGVEQLKQKLGSLLGQTQAGNTVAAVTQIDPVSAHFAELNSLVSQDPS
                     APMAIDGLLADLNELYVQLSSMVGASGEALLGEAKNQASAAAARVNLTAARQPKLVQG
                     LVGSVVAASTNSMMGGVRNQLNAAWQSDVVNVWRQSLSGRYPMVAGSQRDATLDDFGQ
                     FFGVGGVMDSYFRKYLQPYVDTSARNWRWQPGAAQKLGISAGVLQTFQRAALIRDEFF
                     RSGGLQPSVRFDLKPVSMDATISQSQLDLDGQLIGFDHGPSRPVAVQWPNPGSVGVVR
                     LTVSPPVEGGRSALTLEGPWAWFRLLDQSELIGGASPDRFNLRVRLDRSSVAYELRAS
                     SAFNPFKSRAVAGFTLPERL"
     gene            28371..29024
                     /gene="tagF"
                     /locus_tag="VFF22_14985"
     CDS             28371..29024
                     /gene="tagF"
                     /locus_tag="VFF22_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HZX18154.1"
                     /translation="MTMMGFYGKVASRGDFIERNLPKDFQRGWDAWLSAGLQTSQGQL
                     GESWLEAYLTSPLWRFALASGVCGSEAMAGVLMPSIDRVGRYFPLTVACRIPGGQPLQ
                     ALQASEAWFERIEELLLSTLEEGARFEDFETTVLSLEPPPATTLPAYDQAHGLQHWPV
                     VDAPGRLAALAAQACNGASLWWGRGSQRIAAGLRRCQGLPPTESFAAYLLGPEVVAQ"
     gene            29021..29758
                     /locus_tag="VFF22_14990"
     CDS             29021..29758
                     /locus_tag="VFF22_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HZX18155.1"
                     /translation="MTALHYQSAAYSHVGLVRQINEDAYLERPEAGLWVVADGMGGHA
                     AGDYVSSLIVDSLRNIKASDTLSDYEHVLRLTLSQVNTAVREQTHLRGVTMMGSTVVL
                     MVTRGDQGLCLWAGDSRLYRLRAGELQAVSRDHSYVQDLQDSGLLSEAEARLHPRANI
                     VTRAVGVQDQLELACRVFDIQPGDTWLLCSDGLTRTAEDHEIRDVLTHSDPYQVVRSL
                     VHLGLTRGAPDNITAIVIKASEPVSWT"
     gene            29749..32757
                     /locus_tag="VFF22_14995"
     CDS             29749..32757
                     /locus_tag="VFF22_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019818124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZX18156.1"
                     /translation="MDLNIPGFDIDRMIGEGAMATVYLAQQRSLERKVALKIMAPALA
                     ADASFCERFLREGKTLARLAHPHIVTVHDIGNAGQHYYMAMEYLPNGTLSERIAAGLS
                     PEQGLQYIRQIASALGYAHSQGLIHRDVKPANILFRADGTAVLSDFGIAKSLDDSTQF
                     TQVGFAVGTPNYMSPEQARGLDIDGRADLYALGVVLYEILVGELPYMGTDALSTALAH
                     LTQPLPELPLQHSRYQTLLQRLLAKNPDERFNDAGELLAALNAQPAPDSDATVIRPAF
                     TQQTALGAPTTSELGELQPVSIEIPRNAPAPTVAPAPTAPASSAAATPSTGSKGPRMA
                     LLAVAVAAALGLGGGAYWWLSQPSTVVAADSSPHSDGQGAAVAPLAADTGEAGEHPLL
                     MPGKTTIYQRILAKPGAQLLSAPGAAAGEALPAFSVLYVYARRSQDGQSWLQVGAASD
                     GRVQGWVAAEQAADWKQSLVLKFTERSGRAPVLFMQQASDVERLIDRTSLAREQLLQA
                     QNDPEQVPQVVALEPTSSAVPQDQFYLMPIFDFREAFDAEGQPVQLLNIASIDPGSAA
                     QSPADQPAAATDSGFRTGIVLVVDTSVSMQPYIDRVRQVVSELQSQLQARGELDNVSF
                     GLVGYRNSTARTPGLQYLSKTLVSLQEGSDPQRFLRAAEQVQATTVSSHSFNEDAFAG
                     VMQAVEGMDWSSYGGRLILLVSDAGALRKNDPFSSTLMNEAEVRQAALSKQIKIFALH
                     LRTPAGKANHASAEQQYRVLTADSNPQIGDLYVSVPGGDVQAFGERVREIGSTFAELA
                     QQVRAQQAPVPPQLSGGTNLATKSQAIGYAMHMDFLGRRSASQAPQLVSAWTADRDLS
                     NPSLPTLQVCVMLTKLQLNDLQQSLKLIVDAARKTKTSPADFFSEIASASAYMSRDPA
                     ALRQGANLAQSGVLGEYLDGLPYRSKALSMSQDLWLSLSVAEQEDFIDELESKIRLYE
                     TFHNDMANWVRFGNAEPGDALYRVPLSTLP"
     gene            32757..33473
                     /locus_tag="VFF22_15000"
     CDS             32757..33473
                     /locus_tag="VFF22_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010447871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX18157.1"
                     /translation="MIRLQQVLKQRGQGAQRYSLAIEQLQLEAGAQVALVGPSGCGKS
                     TLLDLLALVLAPDAAETFDLELTGTVSDIAALWQQGRQNQLAQLRSQYLGYVLQAGGL
                     LGFLDVRGNIALPRQLLGMADDGQVLRLAQALDVQEQLDKYPAQLSIGQRQRVSCARA
                     LAHGPQLLLADEPTAALDPLNAERVMQLLLAQAEATGATCVVATHDEALAQRAGLTIL
                     RMSCRRDADGGVSAHLEQAA"
     gene            33473..34672
                     /locus_tag="VFF22_15005"
     CDS             33473..34672
                     /locus_tag="VFF22_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZX18158.1"
                     /translation="MRPLLIAGLAWQDYRREARLSACSVLALVAVIAPLLVLFGLKYG
                     LVGSLTERLQRDPAVREIIPLGGTRLQADFIEALGQREDVAFAIPRTRQIAATSELQV
                     TPGEARLNVEMLPTAVGDPLLQGLPMPQTLQQVVLSHTAAEKLGITPGSPLQARFGRQ
                     HGSVLQWQQTTLQVLAVLPLAAFERDALFAQAALLEAVEDYRDGLAVAALDWPGATAT
                     QAPRIYPGFRLYARALEDVEPLRQYLAAQQVQVVTQAAQIAQVQALSRNLDLLFWLIT
                     TLALGGAVAATSAGALAAVERKQRELAVLRLLGVGSRALLLFLIIQALYSGLFASVLS
                     ALLYRLAEGGINHLFVQAPGDYASRLLPEHYLVALAAVLLASALAAALGGRRVARIDA
                     SQGIRDV"
     gene            34665..36467
                     /locus_tag="VFF22_15010"
     CDS             34665..36467
                     /locus_tag="VFF22_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HZX18159.1"
                     /translation="MSDSRFAPSALILATLALAACSPADEPPAASTPATPAQSEAEAP
                     RELDAEQLARLDNPKPLPGDVSLPLPCGGEMLFRHVYVLTEGSLDDREVSLGYPFSEG
                     EAGYKQSFISGYRRDFINGQFSLQDLAPQWQKQIAERLPKIDTGSPLKPMMYFIGKYE
                     VTARQYAQVMTQAQALASGETAPDCAPVTSAAGRLPQVKLSRFEAERFAAVYSAWLLA
                     NNRDLLPVSGRGSKAEEGGAAFVRLPTEVEWEFAARGGSQVSRQELEARLFPRPIEGS
                     DDDGPLANWAVFNQVAGGTGQAPRLMPIGTKQANPLGLFDVIGNAAEMVQESFQLVSA
                     GRRQGTYGGFVVKGGNYLEGEGTLFTGMRREYPLFGADGSEQRNETTGFRVAIGALSA
                     PRSRYDELFAQWQKQGRLAGLTDDIDAADDPTQRLDSIIASAIDPRLQAELGVVNEEL
                     KRNVALIARQREEAAGNLIQSAALVAETINNYNIRLTNLRQSQRQAQQNNDAASAQIF
                     AGAIANGTKALNGAVAIYIDNMASGTRYTNAVIQAQFQRIKEELARKPVIGNSLVSRA
                     TLFVNHVALYRTQQRADPAEILKQLLETQAAAQP"
     gene            36523..37422
                     /gene="tagQ"
                     /locus_tag="VFF22_15015"
     CDS             36523..37422
                     /gene="tagQ"
                     /locus_tag="VFF22_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated lipoprotein
                     TagQ"
                     /protein_id="HZX18160.1"
                     /translation="MRPSKTNLTSQPRSRVLTLAAIGFTSMTVLISGCSTSAVNRVGK
                     STEVAYYPSCYQPVQHLRDTDSNMTRSVATGAVLGAVGGALTGALTADKEDRKRNAAI
                     GAAGGALVGGAAAYYTSKQQQISDDRQRIGSYSTDINASANTFDRSIAYAEASQSCYQ
                     KEFTSLLSQRKSGAINDAEGRKRLAEIIAGLNESNNLITAVNGKAGENLSNYTQAYEA
                     DLKNVGIERAEVTTVAKAPANKPVKQTKVPTEAVSTERVLQQAEAKQTKSQQVAVAGS
                     NQVNSMCSNPDMGDWAPIPCPNV"
     gene            37515..38420
                     /locus_tag="VFF22_15020"
     CDS             37515..38420
                     /locus_tag="VFF22_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019361739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD synthetase"
                     /protein_id="HZX18161.1"
                     /translation="MSDYLIGLRNTPSQIMARQRIEAMNDLRKLYTAIDADPAISGAG
                     VVYIDANFNVVTLREFTPICSIRPKRIIIREAQRNVSPQQFVQQVQSNPRESRLVAES
                     VNTSLACAGAVLGWIAIIGGTAIVPFTGGASLVITTLGVAAASASSVQCVAGAARVYT
                     EVNNPEALDRLDNQQWYQDVSTALDIISLGGVAGAGATILRVMNMRRAMTGRSWYQSL
                     NGLSRQERRRLTNEILENRNPALTPALRKALQRGGELPKRFSTSEISSTLAINIRDAL
                     GAGIGLLGSSMVQTYAIGLYEELEE"
     gene            38417..38863
                     /locus_tag="VFF22_15025"
     CDS             38417..38863
                     /locus_tag="VFF22_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18162.1"
                     /translation="MNQGLDFLSILRRHFPIFISAFFLALFSLAVSQILLLEVYPPSL
                     EDYGIYMMMGSTGISLYLCAANLLVIRGRPWAVWLIVAAMAACILVVIGHWGRRAPDL
                     LYFIGLLLPLSALLTLNSKRYRTMLTAMVEVRGQRTAWRKRNRKRR"
     gene            complement(39036..39569)
                     /locus_tag="VFF22_15030"
     CDS             complement(39036..39569)
                     /locus_tag="VFF22_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HZX18163.1"
                     /translation="MRYLLLPLLFIGTLACAELPETDWLELMPPEDRQALEDMPEISH
                     QGLEQDSGFYNPGGLKQQDQNLPAVMYSSKTVAAFDGKPIRLGGYPVPLETDAAGRST
                     LFFLVPYPGACIHVPPPPPNQLVLVRYPKGIELEDIYAPLWVSGTLKIEQVNNELADA
                     AYALEAGVVRVVEESDL"
     gene            complement(39604..39849)
                     /locus_tag="VFF22_15035"
     CDS             complement(39604..39849)
                     /locus_tag="VFF22_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HZX18164.1"
                     /translation="MSIIGIIFIGLIVGLIARFIKPGNDRMGWIMTILLGVGGSVAAT
                     YGGQALGIYKVGESAGFIGAVIGAVVLLVIYAAVRKD"
     gene            complement(39918..40676)
                     /locus_tag="VFF22_15040"
     CDS             complement(39918..40676)
                     /locus_tag="VFF22_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX18165.1"
                     /translation="MKYLLLLSLLYLPLCHADQVLRADYRERPPEMQALDSLPVGPLI
                     EVLDSAAQRVGARIEWRYAPFVRSLEDLKSGRIDLVPRVLFTAERKDYVHFLPSIGTQ
                     RKSIRFIVKTGQQASLRNYADLQGKVIGVKRGTAYFDQFDGDSALTKSLATDDYLLAG
                     MFRAGRLDVMVVLDAEAIEAQFHKIGFSDYSYAEYQYEQSVGNYYGASMRHYRGEKKA
                     LYEQLGQALRGMRESGEIARIYHRYGVAPPETEE"
     gene            complement(40728..41813)
                     /locus_tag="VFF22_15045"
     CDS             complement(40728..41813)
                     /locus_tag="VFF22_15045"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HZX18166.1"
                     /translation="MKIGVIGGGQLGRMLALAGTPLGMNFAFLDPAPDACAQALGEHI
                     RADYGDQDHLRQLADEVDLVTFEFESVPAETVAFLSQFVPVYPSAEALRIARDRWFEK
                     SMFKDLGIPTPDFADIQSQADLDAAVASIGLPAVMKTRTLGYDGKGQKVLRKPEDVVG
                     AFAELGSVPCILEGFVPFTGEVSLVAVRARDGETRFYPLVHNRHDSGVLALSIASTDH
                     PLQALAEDYVSRVLTKLDYVGVLAFEFFEVDGGLKANEIAPRVHNSGHWTIEGAECSQ
                     FENHLRAVAGLPLGSTAKVGESAMLNFIGVVPPVEQVIAVADCRLHHYGKAFKVGRKV
                     GHATLRCADRVTLNKQIAVVEALIAKA"
     gene            complement(41813..42304)
                     /gene="purE"
                     /locus_tag="VFF22_15050"
     CDS             complement(41813..42304)
                     /gene="purE"
                     /locus_tag="VFF22_15050"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HZX18167.1"
                     /translation="MSALVGVIMGSKSDWSTLSHTAEMLDKLGIPYEVKVVSAHRTPD
                     LLFQYAEQAEERGIQVIIAGAGGAAHLPGMCAAKTHLPVLGVPVQSAVLSGVDSLLSI
                     VQMPAGIPVATLAIGKAGAINAALLAASILGHQHPQFHERLKTFRTEQTESVLENPDP
                     RDA"
BASE COUNT         7419 a        13499 c        13713 g         7731 t
ORIGIN      
        1 gggtattgct ggccatgggc ttggacccag tgtggctagg tgtgatgatc gcgctcaacc
       61 tgcagacgtc ctttctcacc ccgccgttcg gcttcgcgct gttctacctg cgcggtgtca
      121 ccccggcatc ggtgcccacc agcaccatct ataagggtgt ggtgccgttt atcctgatcc
      181 agatcctgct gctggtgatt gcctaccagt tcccggggct gatcacctgg ctgccggaac
      241 aggtctacgg caagtaagcc tgactccagc cacagaacgc ccgtcactga cgggcgtttt
      301 gctttctgcg cgccagctgg gctccaatag ccttctttat tcctacccct gcgagtacgc
      361 caatggccag atcccatgtt gaacgccttg agctggatga gctgacctgc tggcgcgcac
      421 gcgttggtac ccgcgagctg ctgatcagcc agcaaggcgc gcagatactc agctaccagg
      481 aagatgatca accgccgctg atctggctca gcgagcaagc ggcgtacaag cgcggtcagg
      541 gtgtgcgcgg cggcgtgccg gtgtgctggc cctggttcgg cggtctgcaa cgcaacccgc
      601 aggcggtgca ggccatgcat ctgcagcctg ccgctgcgcc cgcccacggc ctagtacgca
      661 acctcgactg gcagctgcaa ggcatcgaca gccgtgatga cggcgtccag ctggagtttg
      721 tctacaacag cgccacccag cccctgaccg actggcccca cgccgccgaa ctgcgcttat
      781 ccattcgcct ggatgagcgt ctgcatatcc agctgagcag ccgcaacctg ggcgacacgc
      841 cactggccat cagccaggcg ctgcacagct attttgccgt cagcgatatc cgcaacgtcg
      901 cagtcgaagg cctgcacggc tgccgctata tcgaaaccct ggaagactgg cagcaacgcc
      961 agcaggacgg cctgctgcag ttcagcggcg agaccgaccg catctaccag ggcaccccag
     1021 cgcagctgag catcctcgac cgcgcctggc agcggcgcat ccagctgcgc agcagcggct
     1081 caagctcggc cgtgctgtgg aacccctgga tcgacaaggc ccagcgcctc tcgcaattcg
     1141 ccgacgatgc ctggaccggc atgctgtgca tcgagcacgc caatgtgctg gatgacaccc
     1201 gcgtgctggc ggcaggtgag cagcatcagc ttgatgtgag cctctggagc gagccctgca
     1261 gcgcttaacc gacctacggt ttaccccact ctcttcaccg agcccgtccg ggctcggcca
     1321 ccgcgcgcag gtcacgtgcg tagttagcaa aagttgcact gcgttacagt taagcgcagt
     1381 acatgcccga cctccgcgac agccactaat tcaccagtaa taaaccactt aaatatagag
     1441 cggtatatcc ctacataaac ttcatgatta atttacttgc gcattaggtc tcacactttt
     1501 caggaactat cgttccagct attagccgcc gaacatcgct cattgttaca tatcccacac
     1561 ttcctacagt tgtccgatat tctttgcaaa aaggggtaga ttttttattt attgaaccac
     1621 aacactagtg actgaaaata aatctgtccc atttttctcc actcaccttg aagaaaaatt
     1681 tacctgaatc gtggcctgcc cctaactccg cagagcccct tttccgcata cggcttacta
     1741 aatatttttt gatttgaaaa actcgtaata tttctttggc tcttccttaa aaaccctagc
     1801 cttgccaaaa tcaaaagcct tcttgaagta gttgaaggct gcatcatgat cattgagctc
     1861 gtaataaact ttccctaggt tcatccaggg gcccacatca atctctgact ccctgatatc
     1921 taactcaata gacgcccaat gcagagcact cttaaagtct tctagttgaa aaaatgactc
     1981 ataaaaacag ccgaccaacc agctgctaat catggtccat tctattttag ggttaggcag
     2041 taggtcccaa gcttgattat aatcggccag tgctttctca tgtgcacctt catctgccat
     2101 cacattgcct gactcaataa tattaattat tttctctgcc aagtctggct gttctacttc
     2161 tagatctctc attttctcac cttagaaata ggttgatttt tcagcaggcc caataaagcc
     2221 tccaggatct ttgtaagttt ccccgagact tgcaccttta gaacgattat gtgaaaagta
     2281 ttctagctca tagttgtctg ggtcacgcat ccatgcccgg acctctttcg acttggcacc
     2341 gtggaatcca cctttcttgt tccaataact gaccgcatct gttttatgtg ccatatcagc
     2401 atgtttgacg ttataccact tcccgtcaga actcttgaat ttcatattgg cgcctgtacc
     2461 tcgaatgcgc ccctcagtcc gcattcgtgc gataacagcc tttccggttt tactattttt
     2521 actcggtgtt ctaccaacgt aactcaagcg acacgaccac cctaacggat ccacccaccc
     2581 atacggctcc ggcgcgtact ggaacaggtt tatgccaccc gccaacccaa tcggatccgg
     2641 gctaataaac ctaccgatat ccggatcgta gaacctgaag gtgttgtagt gcagcccggt
     2701 ttcgcggtcc aggtactggc cctggaagcg caggttctgc tcttcgacaa agaagctttc
     2761 ctgatgttcg gcgagggtgt tgccccagac ctggtagcgg gcctgccaga tgcagcggcc
     2821 gtcgtcttcg gtgagctgct gcggtaaccc attggggtcg gtgtggtaat agcgcaggcg
     2881 cgcgtgttcg ccctggccat cgacacgcgc cagcgggtcg tagctgcctt cgtcgtagag
     2941 gtagaggcta tgccggccgt tgcgggtttc gctgagcagg cgcaggccat cccaggtgaa
     3001 gtcggtctgg ccgagcagta cgccgtgggc atcgtagtgg cttttgccga tacgccggcc
     3061 gagcgggtcg taatgcatga ccacgcggct gccgtcgttg ttggtgactt cccgcacgcg
     3121 gtgttcggcg tcgtagctca ggcgctgcag ctggtgacgg ccactgcgtt tttccaccag
     3181 acggccgaag gcatcgtagc ggtagcgctt gtcctggtag gtgagcagtt tgttgtggcg
     3241 cacccagccg ctgctggcgg catcgccgcc gaccggctgc agcaggttgg cggccgcgtc
     3301 gtagctgtag gtttccagat tgccgcgaat caggtcctgg ctggcgatga tgcggccgct
     3361 ggcatcgtag tgcagctgct gcttctgttc gcgctggctg gccggatcgc ggtcgaggcg
     3421 gccgattagg ttgtcgctgg ggtcgtaggc gtagcgccgc tcggcctcgg ccggcagttg
     3481 cacgggttgc tccaggcgcc ggcgcaggcg cgcggtgagg cggccgctgc ggtcgtactg
     3541 actgcgggtg acgagttgcc cctgggtgcg tagcacctcg cggtgcaggc ggtcgcgctc
     3601 gaagcgactg accagttgat catccaggct gagcagttgc aggtggccgc tgccgtagta
     3661 caggcggcgg atacggcgct ggtcaggcaa ttgcgtttcc agcaggttgc cgagctcgtc
     3721 gtaggcatgc tgcagggcgc cagcagcgct ctgctcgctg agcagttggc cgagggcatc
     3781 gtaggcaaag gccagcgact gcacctggcc gtcgacggcg gtgaggtcga tgctgagcag
     3841 gcggtcgagt gggtcgtagg cgtattcggt gcgaccgtca tcggtgtgct tggcgatcat
     3901 ccggccaacg gcatcgcgct cgaagcggtg gatgatcggc accggcggcg cctcggggac
     3961 gctacccagg ccagtgccat ggggcgccgg ggtataggcc aggccgatga gattgccctg
     4021 cgggtcatag cggtaatggc gggcactgtc gtcgaggtcg tgctgggcgg agaggcggtc
     4081 gagcacgtcc caggtgaagc ggtagcgctc gtcgttctcg ttgctcaggg cgagcaggcg
     4141 gccataggcg tcgtattcga aaccgactct gcggcccagg gcatcgatgc gctgacgcac
     4201 ctggccacgg cggtcatagc gatagcgcac gggcttgccg gcaggatcga ggtaggcgct
     4261 gagctggcca atggcgtcgc gttgaaactc ctcgctgcgg ccatcggcca gctgcgtctg
     4321 cagcaggcgt ccctggctgt cgtagtggta gcgggtgatc tcgccgaggg catcggtgat
     4381 ctgcaccagg ttgccgtgct cgtcgtaggc gaagcgcgtg gggtagccgg agcagtcgac
     4441 ctgttcggtg agctggccga gggcattcca gcgcagggtc ttgcgcttgc cgctggcgtc
     4501 gatgatctcc accggcaggc cacttgtgtc atagcggtag cgggtgacat ggcccagcgg
     4561 gtcgatctct gcgatgcagt tgccgcgctt gtcgtagcgg tactgccagg cattgccggc
     4621 ggcgtccgtc tcggcttcgg gcagcgacca gtggccgagc cattgggtga agtcgcgacg
     4681 ccccagcggg tcgagggttt cgcagaggtt gccggactcg tcataggcaa attgccaacg
     4741 cgcgccgtcc ggtgcgacgg cggccagcag ctggcgctcg tcgttccatt cgaacagcca
     4801 gagctggccg agggcgtcgc gatagcgggt gatctggtac tggttgttcc agtggcgttc
     4861 gctgatacgg ccaaggccgt cggtgacccg ggtgaccccc gcatccagat cgtaatgcag
     4921 gtggtactcg tcgccgtcgt cggtccagtg ccgtgtgacc cgccattcgc ggccatcagc
     4981 accctgaaac tcgctccatt ggtagaagca acgcaggccg gtaggacgct ggtgttcgat
     5041 cagacggtgg ccgccgtcat agacgaagcg gcgcagcagc tggcccttgt cgtcacggac
     5101 ttcgctgaga tcgccctgag tgtcataggc gtaggcgacc agcacctcgc gccgctcatc
     5161 ggcaaacagg cgctcgaccc ggcccaggcg ctgcgggtgc tgggcgtcgg caatcagttc
     5221 gatgcacagc tggttgtggc catcgcgcac ggcacgcagc cggccagtag cgtcgtattc
     5281 gagcagcagg cggttgtcgt tgcgatcgcc cagcttgctc aggcgcagac gttcaggtga
     5341 ctcgggcaac ggctcgaaca ggcggtagag gccatcgacg ctttccacca gcagcgcacc
     5401 gctttcatcg cgacggaagt tcaggccttc acccgggctg tagcaggcat cgcccggcgg
     5461 cagactaccg atctcgatgg ggcgaccctg ctcgtcgata taggtcagcg cttcgccacc
     5521 ctccgggtgg gcgcgtacct gcacggcgat ctcgaacggc aggctccagc cggcacccaa
     5581 caggccgtca cggcgctcgt cgcagctgct gtagaagcgc tgccactcga tgggcatcag
     5641 ccccggcagg acgaagtcga gatcgtcgtc ttcgccgagc accttggccc cggtgctggc
     5701 gtgcaccggg ttgggtgcac cgctgctggc cgaaccaagg gcgttgacga tggaattggt
     5761 ggcataggcg gtcgcgccac ccatggccat gttgccggcg aaaccggcgg ccaggcaggg
     5821 cagcttgctg cacagcttgc gcgggttgcc gcgcagtgcc atgagcacgg cgagggccag
     5881 gccgataccg ggggtcttgc cactgcggat cggctgcacc accacggcgt caccaccgat
     5941 gcgcacgttc ggcgagatgt tgccgctttc caccaccgtg gcctcgcagg tgctgcgatc
     6001 accgctgcgc actgccggct ggccttcgat gctgaccttg atcgagcctt cggcgagcaa
     6061 ttgcacgggc atcggcgggt gcttcttgca ctcgatggca tccagcgggc aggggatgac
     6121 gccaggtgcc ggctgggcaa ccgtcggctg ccacatctcg ctgaagaagc tcttggccat
     6181 gtcgaggaag ctcggctcag gctcctcggc gggcggtgcc tgctccagct cggtgccgga
     6241 cggcgcagcg gcgctgccca acttgccagc ggcgcgcgcg gcaggcaggc cgttggtttt
     6301 ggtcttgttg gcgccggtga cgatcttggc ctggacattg ggcggggaga tggcgttgcc
     6361 caggccttcg cagagggtcg tgagaccctt gtcccagccg gttttgttca tgccgacccc
     6421 cacggcgata cccactactg cggcgagggc aaatccaccg agaccgccgg tgaggacggt
     6481 caggccggtg gcggcgacca ccgcagcgca ggccacggcg gtgatggcgg cataggcggc
     6541 cacttccagc acgccgccga cgatgtcggc aagcagtgtg gtgtgcagca gatcgtcgcc
     6601 gagccgggcg gcccagagcg catcagacat cgcaggtcat ccgttgaggc tgaggctgtg
     6661 cttgatctgc gcccagtgcg cggcgtcggc atcatcgagc ggtgtgggtt tgacgtagct
     6721 gagggcgagc aggcggcgac catcggccag cagcacaccg atctggaact ggtagacctg
     6781 ctccggcccc ttgctgaact ggctgcgcag ctcgacgccg acgatggcct gggccgggcc
     6841 gacctggaca tcctgcaggg cgctgcagcg cagatccttg acctgtttcg ccaacttggc
     6901 gacctgggcc tcgaagtggc tgcgcagggt ttcgccgtcg cccaatgcac cacgggtgat
     6961 taccagcgag gtgccgagat cattgaggcg caggatgttg atggtgctgt cctgcaggtc
     7021 cttgccgggc agttgcaggc tcagctcttg ggtggtgtag ctcatcagga gtttcctttc
     7081 tcttgcccga acagggattt gacggctgca tcgatgctgc cctggtcacc ctgaccatcc
     7141 ggggtggcac cagggccgcc gctgatgttc agattgagca cgccgcctgt gttgaattcg
     7201 gcgtcgccac tggcgtggaa gttgatcgcc acgccgctga ggttgatctg gccgctggcg
     7261 ttgagttcga gaatgctctt gccgcagacc aggcgcaggt tctggccgac ctcgatgagg
     7321 tagctctggc tgatgctgtc ggtcttggtc gaacccacca gcagcacatc gtcgagcttc
     7381 accgcgcgca ggcggttgtt gccgatggtc acggtctcgt tcatgccgat gctcttgacc
     7441 cggttgttgc ccaccgagtg ggtttcatcc acctcgacga cgatgtcctg gttgcgctcg
     7501 gcgtggatgt acagctgctc ggcgcccttc ttgtcctcca tgcggatttc gttgaagttg
     7561 gccggcgtgc cgcccttgct ggagcggctc ttgatgccgc tctgggtagc gttggcgggc
     7621 aggtcgtagg gcacggtctg ctcggcgttg tagacgcgcc cggtgatgat cggctggtcg
     7681 gggtcacctt cgaggaagct gacgatgact tcctggccga tgcgcggaat ctggatatgg
     7741 ccccagttct taccggccca ggactgcgaa acgcgaatcc agcaggagct gttttcgttg
     7801 gactggtcgt ggcgatccca gtggaagtgc accttgaccc ggccgtactg gtcggtccag
     7861 atctcctcgc cggcggggcc gaccaccacg gcggtctgcg gcccctgcac catgggcttg
     7921 ggcgtgccgc acagcgggcg gaaggtctgc tgggcgtcgg tgcaggtgag ctggcactcg
     7981 aactggccac cgccgccact gccgctctcc agggattcct gcgcaacgtg atagcgcgcg
     8041 cacaccacca ggtactcacg gttctggtcg gcgcggccga agccggtgag gctgaacagg
     8101 ttgcccgagg cgatgccgcg cacgctgccg ccgacctcga cgcgctcgtg caggctctgc
     8161 agcgcctcga tacgattgcg cgcgtactgc tcaccatcgc tgctctgcac gtaggtaccg
     8221 ggatagtcgt acagcggata gtcgccggcg ctgtgcggac ggggcatgac cgaacgcacg
     8281 tcgatgcgcg cgctgggccg ctggaagtcg tagtcgttga gctccatcga gcccggctgc
     8341 acgtgctggg ccagctgcca gtcgttgatg tggtcgcgct cgcggtgctg cccatcaggc
     8401 gggtagtaag gcacgctggc gtaaccgggg gccggcacat gggcgccgta ggcgtcggcg
     8461 agcaccagca cgtggcgatc ggcctcgtgg cggaagtagt agtagatgcc ctccttttcc
     8521 atcaaccgac tgacgaagtc gaagctggtt tcgcggtact gcacgcagta ctcccactcg
     8581 cgataggggc gactgagtgc gtcttcgaaa tcggagaaac ccaggtcgcg gaacacctgc
     8641 ttgatgatct gcggcacgct catgtgctgg aagatgcggc agtcggaggt acggctgagc
     8701 aaccacagcc aaggtcgcag ggtgacccga taggcagcga actggccacg gtcgacggtc
     8761 tgcccgcagg cggcgacgat gccatggaaa tagcgttcgc caccgccatg ctgctgcacc
     8821 gagagcgtca gcggcttgcc gagcaactgg ttgagatcga ggttggcgtc gtgggacacc
     8881 agctgcagct cgtattcgaa caggcggccc agctcgtcgc tgccgtgcat cgagcgcagc
     8941 agcagaacgt ccggcccgag cgggctggtg atgcgggcaa agcgggaaga ttgggcgaat
     9001 agcataggtc ggggttccag cctgccatgg cgcgggccgg caaaagccga cccgcgctcg
     9061 ggctcaggcg ttgtcggcga agctgtagtt caggtcgttg tcggccacgc tgatgcgcac
     9121 gccggccagc ggtttgcctt ccatcatgcg ggtgaggaac tcgcggctca tgtccggcag
     9181 catgccgttg gtaagaatgg cgtcgatctg ccggccgccg ctctcgctct cggtgcagcg
     9241 cgagaccacc aggtcgacca ccgcctcgtc gtagtcgaac ggcaccttgt gggtgctctc
     9301 gatgcgcttc ttgatgcggc ccagttgcag gcgggtgatg gccttgagca tggtgtcgct
     9361 gagcgggtag taggggatgg tgaccagacg gccgagcagg gcgggcggga agatctccag
     9421 caacggctga cgcaggtcgc gggcgatgcc ctccgggtcg ggcaggttgt ccgggtcctg
     9481 gcacaggcgc gccacctgct cggtgccggc gttggtggtg agcaggatca gggtgttctt
     9541 gaagtcgatc aggcgccctt cgccgtcttc catcacgccc ttgtcgaaca cctggaagaa
     9601 gatctcgtgc acgtccgggt gggccttctc cacctcgtca agcagcacca cgctgtacgg
     9661 ccggcggcgc acggcctcgg tcagcacgcc gccttcgcca tagccgacat agccgggcgg
     9721 cgcgcccttg agggtggaaa cggtgtgcgc ctcctggaat tcgctcatgt tgatggtgat
     9781 caggttctgc tcaccgccat acagcgcctc ggccagcgcc agtgcggttt cggtcttgcc
     9841 gacgccggag gtgccggcga gcatgaacac gccaatcggc ttgctggggt tgtcgagacc
     9901 ggcgcgggag gtctggatgc gcttggcgat catcttcagc gcatggtcct gaccgatgat
     9961 gcgcttggcc agcaggctgt cgaggttgag cacggtctcg acctcgttgc gcgccatgcg
    10021 cccgaccgga ataccggtcc agtcggccac caccgaggcc acggcctggt agtcgacagt
    10081 cggcagaatc agcggagttt caccctgtag ctggctcagg cgctgctgca gctcgaccag
    10141 ttgggcgcgc aggccctggt ggtcgctgtc actgtcctga tcgaccatgc cgacgctttc
    10201 gcgcaaacgg gcgcgggtgg ccagcagttc atcgaccagt tgcttctctt cggcccagcg
    10261 gctttccagc tcggccagac gctcgcgctc ggccgccagc aggctctcgc tgttggcctg
    10321 acgcaggctg acgtcgatgc cgatggcatg ctcgcgggcg atgatctgca gttcggtttc
    10381 cagcgcctcg atgcgccggc ggctgtcgtc gacctcggcc ggcacggcgt gcaggctgat
    10441 ggctacgcgg gcgcaggcgg tatcgagcag gctgaccgac ttgtccggta gctgacgtgc
    10501 cgggatgtag cggtgcgaca gcttgaccgc ggcctccagg gcttcgtcga gaatctgcac
    10561 ctggtgatgc ttctccatgg tcgaggccac gccgcgcatc atcagcagcg ccttgtcctc
    10621 ggagggctca ggcacctgca ccacctggaa gcgtcgggtc agcgccgggt ccttctcgat
    10681 gtgcttcttg tactcggccc aggtggtggc cgctactgtg cgcaggctgc cgcgcgccag
    10741 cgctggcttg agcaggttgg cagcatcacc ggtaccggcc gcgccaccgg cgccgaccag
    10801 ggtgtgggct tcgtcgatga acaggatgat cggcttgggc gaggcctgca cgtcttcgat
    10861 gacctggcgc agacgctgct cgaactcgcc tttcatgctg gcgccggcct gcagcaggcc
    10921 gacgtccagg ctgcgcagct cgacgtcctt gagtgccggc ggcacgtcac cggcggcgat
    10981 gcgcagggcg aaaccctcga ccacggcggt cttgcccacg ccggcttcac cggtgaggat
    11041 cgggttgttc tgccgacgac gcatgaggat atcgaccagt tggcggattt cctcgtcgcg
    11101 gccgacgatg gggtcgagct cgcccttgcg cgcctgctcg gtgaggtcga gggtgaaacg
    11161 cttgagcgcc tcctgcttgc ccatcgccgc cggcgccatg gcgccactgg cctcgcccgg
    11221 tgcggcagcg gcaaaaccgt cgctggcggc caggttgttc tccggcgaat cgccgacgta
    11281 ttcgtcgaag cgctcgctca gggtctccac cttgatcttg gcgaactcgc ccgacaggcc
    11341 cagcagcacc tgacgcaggc tcggcgtctt gaggatgccg atgatcaggt agccggtgcg
    11401 cacctgatgc tcgccgaaca tcaggctgcc gtagacccag ccacgctcga ccgcttcctc
    11461 gacgtgcgag gacaggtcgg tgatcgaggt tgagccgcgc ggcaggcggt cgagtgcgtc
    11521 ggtcaggtca cgcgccaggc gtgccggctc caggctgaac tggcggatga tgcggcgtag
    11581 gtcggagtcc tgcagctgca ggagctgatg gaaccagtgc gccagttcga cataggggtt
    11641 gccgcgcagc ttgcagaaca cggtggcggc ctcgatggcc ttgtaaccga cgctgttgag
    11701 tttgccgaac agcgcggtac gactgatttc acccatgtgg agctctcctt gattgatccg
    11761 ccggcggggt cgctgcggcg tgatggcgtg ccagcaccag atccctggcg tcgtgctgcg
    11821 gctggccaag ccaggtggtg tagcccaggc gagcctggcc gttgaggcat agcgcgggga
    11881 cttcctcgcg ctgcaatacg agattcagtt gccagtcgag ttcgtcgccc tgatattcgg
    11941 caacccaggc caccagcgcg gcgaaggact caccatcggg cagcatggcg ttgtactgct
    12001 caagcgtcag tggaccgaag cagatgcgga agttgtgctg gcggtcccag acctggctgc
    12061 ccaggcagaa gtccacgccc agctggctgg actccacgcc caggcgcgtc cgctcgggca
    12121 ggcgtagcca gctgccggcg tactcttcga tctgcaccgg cacgccgaag tagtcggcga
    12181 ggatcgcctt gaggccatcg gggtagcgcg tctgcgcggc caggtgaccg ctgaagtgca
    12241 gcttcgccgc atcgtccagc aagcctcgct ggcgcaagct gggcatacca cgaccgctga
    12301 aggcggccag gcgcgccgac caatagtcgt cgtcggggcg gtcgtggctg acagtcggtc
    12361 gggcttcggc ccaggcgcga tagaacaggg tcagcaggcg atgatggaac agatcgagga
    12421 agtgcttgct ggtgctgtcg gcgtggttgc gctggcgctc gcgcacgtac tcggtcaggt
    12481 gcagcggcag cggcccgttg ggcccgccga ggccgaagaa atactgttcc agacgcgccg
    12541 ggcggtcgcc gctggcgggg gtcacgccgg ccagggtggc gggggcgaac gtgccttcct
    12601 ggcgctgccc gaggcgcagt ggatcgtcag ccaggcgccg ggaacgaccc aggcgcggca
    12661 gatgcgggtg ctcgttctcg atgcgccgca gggcctggaa gaagtcgtac tcccagggct
    12721 gcgcctgcat ctgcacaagc gtgtctagag gttcggtcga cggccgggcc gggctttcca
    12781 tcgcatgatc tcgccgcgct cggtgctgcg tagcaccgtc tcggtaaagc tgttgaggga
    12841 tacgtagcgc gccaggaagc gctcgaacac cgcaccgagg agaaacaccc cggtaccgcg
    12901 aaaagcgttc tcgtcgaact ccagcgtgat ttccaggccc cgaccgaaga ctatcggccc
    12961 gggcatcggc aggcgccgcg tacaaggctt ggcactgact tcgcgcaggc cttcgatctg
    13021 cagttgcagc gccggattct gcatgtcgcc atacaggcgc agcatttcgc gcagggcagc
    13081 ggcgccctgg ccctgctcgg ccagtgacag gtagttcagt gacaactggc tgatcaaccg
    13141 ccaggcctgg ttgtcgtggg ccgggctggc acgcggccgg ctcggcccgg ccacgcagcg
    13201 cacgctctgc accggtgcgc tatcggccag ggtgaagtcg ctcttaccga tgccgacggt
    13261 catgaacaac ggcaggtcgc ggttggtgca cagggcactg agccccagct ggcgcaggtc
    13321 atggcgatac ggcgcctggt ggctgtcgac caggctgatg aaggtttcgc tgccgacata
    13381 gctggagcgg gtgccgctgc ggcgctgctc ggccgacagc acacggggtt cgcggcgcac
    13441 gctgtagtag gcctggtcac ggccatagcg agcggggtcg cgcacggcat agaacggcag
    13501 gaagggctgc tccgggccac tgccatggcc ggtgacgcca cgcagcgagt ggatctcgaa
    13561 atccatcggc cgcgtacggt cggcgatggc gtggtgctcg tgcaccttgt cgctcaggtg
    13621 gatgcgatcc aggcgccgcg ggaacaggtt gatcgccggg gtacagaacg gcaggaactg
    13681 actggcgccg acctgattct gcaagtggct gtccaggcgg tcgaacagca cgatcaactc
    13741 cagctcctca ccgctgcact ggcgtacggc acggcgcaga ccacagaagt cgacgaacag
    13801 aaaacgctgc ggcagggcga agtattcctg cagcaggcga taaccctgga aggcctgtgg
    13861 caccactggc agggccgcct cagcatcatc gaagccgcag gggcgcaggg cgtcggccgg
    13921 caggcgctcg acccagtccc gccctggctg gcgggcgaat accgcgatgc ggttgcccag
    13981 cagttgttcg tagagacgaa acggcagctc gtcgaggccg ctgaggtaca gcggcaggtt
    14041 gtccagatcc aggctggaga agggtatttc agcgccactg cgcagggtga tgcgtagcgc
    14101 agccttggcc cgcggttcgc tggcggcaag gcgacccagc gcggcggctg gattgctgat
    14161 gtactcggca tccgccactt gcagcggcca cagggtcacc gggtgagccg tgcggtattc
    14221 gcagggggtc tggctgtcct ttccgagggt ggcacgcaac acgctgtcgc gcggcaggtt
    14281 gaagctgccg ttgagcgcgc catcgttggc atcggtgtgc agctgtacca cggtcatcga
    14341 cggcgtcggc gccaggtaat gcgggtaggc gacttccagc aggttatggg tgaaggtcgg
    14401 gtactcggca tcgagcttca gttgcacgcg ggcggtcagg taggcgaagc cttcgagcag
    14461 gcgctcgaca taggggtcgg cgcactccat tcccgacagc gccagacgcc cggcgatctt
    14521 cgggtattcg cgggcaaact cctgggcgct ttcgcggatg tgcagcagtt cctggttata
    14581 cagctccagc agacgggggt tcatgaacgt ctccgctgct cggcgggcag cacctggaca
    14641 tggccggact ccaggtccag ctcggtgcgc agcagcaagg gtagggccac cggctgtgcc
    14701 cacaggtcac cctgaatctc gaaactcagg gcgttatggt tcatctgttc gtgaccgaca
    14761 tgcgcccgta cccgcacgct gtgggcgagg atgcgcggct cgaaggtgat gatggcgcgc
    14821 tggatcaggt tctccagggc ggcgatatcg atcccggaaa cactgctgcc ggccaacggc
    14881 ggcaggccat agttgatcac cgaggtgcct gccggcgtgt gccgcgtggt gtcggcattg
    14941 agcagggcga tggtattgaa cagccaggtc aggtcgcgca gcaccgaagc cttgagctgg
    15001 ttcatcgaca gcacgcggcg atccgcgctt tcctgcggat tgccagggtc atcgtcggtc
    15061 aggcggtcga gcagcgatgg ctgcaggcgc tcgcgcgggg tcatggaggt taccaatcga
    15121 acatcccgac gaaggccttc tggtcccact gactcggacc gcaggcggcg atgcggctga
    15181 ccggcgcggc ctccagttgc ccctgatgca gccgcagcgc ctgcaggtcg gtcaagcaac
    15241 tcctgcttgg cgctggttgc ggctcgctgc gctcgaccat gatgatcggc gtctggcgga
    15301 agtactcgac ccgcagttgc ttttcggggg tttcactgaa acggcagggc cactgcatgt
    15361 ccagggtcag cggcgcagcg tccgccaggg ccagagcgca gcggccctgg gcatcgctga
    15421 gccgcacgga ctggcccgcg aattcggcct gggtgattcc aaccgagggg ctggcgccgt
    15481 gctcggcaca ggcgctgaga ctgaccagca gcgttgccag caaagcattg agcggcagga
    15541 tacgcatcag ctcagctccc agaaccagac ggccttggcc cggtggaagt aatcaccggt
    15601 caatgtcagc ccacggttcc acaggtcgat atggtcccca ctgggacggc cctccggatc
    15661 cgtgggctga taatagaaat ccttgtagaa aatgatcccg gtcttgccga gcaggctggt
    15721 tctgtccgcc gcgctgttgc tgtagatcct gggagcgccc agctgcggtc gtttccacag
    15781 ccagttggcc agggattcgg caccacgagc atgaccgtga gcacagcgcg gctcgctgta
    15841 ggtcgaggag ttgatctgca acgtgcgctc gttgttcagc gcgagactca gacgaatggc
    15901 gcactggttg gcccagttgc cgtcgcaagg tttgcggtcc ggatactcga ccagcaagtc
    15961 ctcgtaggct ttccatagat tgatgaacga aggcttggac atgacgaggt ctctttggcg
    16021 taacggtctc agcttggtca gcccggcaac cgccagcggc tcgctgaagg catccacctc
    16081 caggccaggg cctggaggtg ggaggcatca gatcttgacg ttggaacgga tattccagcc
    16141 gtacttgatc gggccgcctt ccttggtgcc atcggctttt tgcggctgat agtcgacggt
    16201 gaccttggcg aagttcaggg tgatgttctc gatcagacgg tcatcgttgc ccgagccgcc
    16261 agtgctcagc gaggagatca gcacttcttc gaggttgatg atcaggtact cgacctggga
    16321 gtcaccgcct gccttgcgta cggtcagggt gaccttgggc acgtgcttgc cactggagca
    16381 atgcatcatc aggttggggg tggccttgtc gacaaatttg gtcagcgaca tatcctggat
    16441 gctgaccttg cccgcgccac cgccagtacc ggtgtgcatg ttgccggact gggacatgcc
    16501 ccagctccag ttgagtactt cgatttcgtc cttgtgggtc ttgtcctgag actcgccctt
    16561 gatgtcaccg atcttgatga agatatcgac agccatgttt tctcctgggt gtggtcattg
    16621 ccactagggt cagagattgc gaaaagcccg gggctttcca tgtatcaggc agaggcctca
    16681 cccggcggtg ccgcgtgaga ccggcttgcc cgccggggct caggcgccct tggccgacgg
    16741 cagcttggaa accagccgca gcgacaccgt cagcccttcg agctggtagt gcgggcgcag
    16801 gtagaacttc gagttgtagt aaccggggtt gccctcgacc tcctccacca ccacttcggc
    16861 ggcggccagc ggatgctgcg ccttggtggt ctcggtggag tgagccgggt caccgtcgac
    16921 gtacttgagg atccagtcct gcagccagag ctgcatttcg tcccgctcct tgaacgagcc
    16981 gatcttgtcg cgaacgatgc acttgaggta atgcgcgaaa cggcaggtgg cgaacaggta
    17041 cggcagacgc gcagccaggt tggcgttggc ggtggcgtcc ggatcgtcgt actcggccgg
    17101 tttctgcagc gactgcgcac cgatgaaggc ggcgaagtcg gtgttcttct tgtgcagcag
    17161 cggcatgaag ccgttcttcg ccagttcggc ctcgcggcgg tcggagatgg cgatctcggt
    17221 cgggcacttc atgtccacgc caccgtcgtc ggtggggaag gtgtgcgctg gcaggttcgg
    17281 cacttcgccg ccggactcca cgccacggat gcgcgaacac cagccgtagt gcttgaacga
    17341 gcggttgatg ttcaccgcca tggcgtaggc ggcattggcc caggtgtact tgctgctgtc
    17401 ggcgccgtcg gtgttttcct cgaaggcgaa ggcttccacc ggatcggtct tggcaccata
    17461 cggcaggcgt gccaggaagc gcggcatggt caggccgatg tagcgcgaat cctcggactc
    17521 gcgcagcgag cgccagccgg cgtattccgg ggtggtgaag attttggtca ggtcgcgcgg
    17581 gttggacagc tcctgccagg agcccatgcc catcacggtc ggcgaggcgg cggcgatgaa
    17641 cggggcgtgc atggccgcgc agactttcga cagttcgccc agcagctcga catcgggtgg
    17701 cgactggtcg aagtagtagt cgcccaccag gcagccgtag ggttcgccgc cgaactggcc
    17761 gtattcttct tcgtacatct tcttgaagat cgggctctgg tcccacgccg tgcccttgaa
    17821 tttcttcagg gtcttgtgca gctccggctt ggcgatgttg agcacgcgaa tcttcagctg
    17881 ctcatcgctc tcggtgttgt tgaccaggta gtgcaggcca cgccaggcac tttccacctg
    17941 ctggaaatcg ctgtggtgga tgatctggtt gacctgtgcg gtgagcttgg catcgatggc
    18001 ggcgatgatc gactcgatcg acttgatcgc atcgttggag atcagatcag tctgcgccag
    18061 ggcctgctcg gccagggtac gcacggcggt ttccaccgct tccttggcgc gttcggtctt
    18121 gggtttgaat tcctgcatca gcagcgaagc gaattcgctg gtctgctcgg tgctggccag
    18181 tgcggcctgg tcttgttgca actgttcgct catggttgtc ttccgaatca ggcctggggc
    18241 tcgttgtcct ggggtttcgg cgcgctagcc agggcctgca gcagtgccgg gtccttgatc
    18301 gccttcatga tgatgtcttc ggcgccggtc ttgccatcca tgtaggtcag caagttggcc
    18361 agctgggtac gggcttccag cagctgcttg agggcatcga cctggcgtgc cacggcggcc
    18421 gggctgaagt cgtccatgct ctggaaggtc atgtcgatgc tcaggttgcc ttcgccggtc
    18481 agctcgttag gcacgttgaa cgccacacgc ggcttcatcg ccttgaggcg cgagtcgaag
    18541 ttgtcgacgt caacctccag gaacttgcgc tcggctactg gcgccaaggg ctcggccggc
    18601 ttaccggtga ggtcagacat gacgcccatg acgaacggca gctgcacctt cttctcggcg
    18661 ccgtacagct cgacgtcgta ctcaatctgc acccgtggcg cgcggttgcg cgcgatgaat
    18721 ttctgagaac tactgttcgc catggttttc tcctggtcgc ctgacgcgac gcggcgtgtc
    18781 ggccttcggc catctacggt gaagcgtgat cccgcatgcc aggccctatc aggcctggtc
    18841 tgcgtcgggg ccgcgcaggt tttcaaactg gctcatgcca tcgggaatca ggttgcgcac
    18901 gatggtcgcg aagtcggcat tgaccaggct gcgagcccgg tttagcagta ccggcaacgg
    18961 gctggatggc tcgtggcggg cgtagtaggt gaggatccgc tccaggcttc tgagcacatc
    19021 atcgcgattg ctgatttcgc cgctaacgcg cgctacccca gaccccgacg cgtgctgcgc
    19081 cagctgctct tgcgcagcct cgccgcctgc cgggtaatcc tctgaacttg cccctgccgg
    19141 gctgtcgccg agcacctgca gggcaagctt gagcggctgc tgcagcgcag agaaatccac
    19201 gccctgagcg gagccgacct gttcgctgac caattgctcg atggcattca agctgtgcag
    19261 cgcacgcttc agggcttcgt gagtgagctg cagggcttcg ctgtcggcgt cctgcagggc
    19321 gccgtcgagt tcttcgatgc tgaggctttc gctgctggcc aggtgcaggc cggcggcatt
    19381 cagcaccgcg cgcagggtga tgttgccgaa cacccgtgaa cggaccagca cagcactgcg
    19441 cagcaggccg agattggtct cgcaggtcag cgcggagagg gtgttgatgc gtacggtggg
    19501 gtcgttgtcg tcatcggcat cgagctgcgg gtgaatctcg ggccagtagc gcgagagcag
    19561 ttcgaggatc aattcgaggc tgtcggcaag gccttcgaca ccgcgcaaag ccaggcgact
    19621 ttgcagcaga tagcgagtaa tacgcagatc cttgctgcgc agcagcagga ggtgacaggc
    19681 ctgttccagc tggcgccaat cgggcggctc agcagcctgg atggcatcac ccatgctgcg
    19741 ttcggggcgc cccagagcat cacgctcaag ctggagaaaa tcgctgtcgt attccaaatc
    19801 ttcgccgcat ggcgaagcat ctgaaatagc ggtgagcaac aacgaagtct ccacgttttc
    19861 gatctcctta tcgattgagc tgcgtcacag aaggtgccgc aacttcatcc taacttcatc
    19921 ctagttggaa ctttgccaat taatgcctaa tgctaattgg ccaccacaga gtcccggtgg
    19981 tgccgcgcat tttttaactt aaaaatatat aaagtcaaga tacaatggcg cccatcacga
    20041 acctttgtga actttggcat agtttcgcta agttgtgggc ttgagcacat acttgaacca
    20101 cgacacaata ggtaagatcg cctgctgcag cgtaacaaag cccgtttaat ggacgttcca
    20161 caagggctaa aagagacctg aaagccttta aatcaaaggc tctcatggtt tttagggtgt
    20221 cacaattagc cgggcaagga ggccacatgg cgctgcgttt gaccatcacc agctatcaca
    20281 agatcacccc cggtcaatgt cccgagaaaa ccttggaaca tggcgtgatg gcaattggcc
    20341 gcagcccgga taatgattgg gtattaccag acccggcgcg cctagtttcg tcgaaacatt
    20401 gccttgtgca aaataaggat ggccgctact acatcaccga cagcagcacc aatggcgtgg
    20461 agctggtcaa ggctggtgtg cgcatgcagc gcggtaacag tgaacttctg aatcacggcg
    20521 aagttattcg catcggtaaa tacgaaattc aggtagcgct ggaaagttcc attgcagaag
    20581 gctccgcagc gttttccgcc gccccttccg ggagtagttt cgaagccctg atggcgggcc
    20641 atcaggacgc tccggccgta cctttcagcc ctccggcaaa ccccttcgat tcctcggcca
    20701 gcgcccacct gcagagcgtc tccccgcacg aaacactgcc ggatctgttc gattttctcg
    20761 acccgagcac atcggcgccg aagcccattg ccgaccacgt cccggcgcag cagcatgact
    20821 tccgtccgcc ggaggtcagc ccccctccag tcgagcagaa gccaagtgac gaacctggcc
    20881 taatccccga cgactgggac ctgctcgacg acatcacgcc ggctgcgaca accccggcag
    20941 catcgctgcc cgtggacctc tccctgccgc cagagccgac cgagccggca cctttgccgc
    21001 ctctgcctgc agtacctgac cccactcctc cccctgccac cagccagcct cagcttgctc
    21061 aggacgacga tgtgctgctg caggcctttc tccgtggcgc cgggcttgag cgcctgcgcc
    21121 tggataccca gcgtgcagcc gcgcagatgg aaagcatcgg acgcagttac cgcttgatgg
    21181 tcgatggttt gatcgacgtg ctgcgtgcgc gcagcagcct caagggcgag ttccgcctac
    21241 agcaaaccat ggtgcagccg gtggagaaca acccgttgaa gttcgccccc aacgccgaag
    21301 aagccctgct cctgctgctg cgcggcggca actcggcctt catgtcggct gatctagcgg
    21361 tgcaggacag cttcgatgat ctgcgcgccc atcaattggc ggtcatggcg ggcgtacagg
    21421 cggccattcg tcacctgctg gcacgcttcg agccgcaggc actggaagcg cgcatgggca
    21481 aggggggggg cttggccagc ctgctgccga acgctcgcca agcgcagtgc tggcagcagt
    21541 tcgtggagct ctacgcgacc ctgtcgcgtg aggccgaaga cgattttcag gacctgttcg
    21601 gccgcgagtt cggccgggcc tatgagcaac atatcgcccg tcaacgtcgc ccttgagggc
    21661 gtaggcaagg atgagcaacg tgcgtaaacg aacactccgg cccgttctgt tgggccttct
    21721 gctggcagcg ctgaacagtg gctgcagcaa ggacgcgatg gataacgccg acactgcgca
    21781 ggggccgacc accgtcgtgc tgcagctgca ggctggcgat gaactcaacc ccaccaccga
    21841 cggccacagc gcgcccgtgc gcgtgcgcct gctagagctg cgcagcgccg cagcgttcag
    21901 tcggacggac tacttcagcc tgaccgaacg tgcggcggcg agcctcggca acgaattggt
    21961 ggcagaggat gaatggctgg tgcatcccgg ccaagcccgt gagctgaccc gtgtgctgaa
    22021 tccggcaacc cgccatctcg gcctgctggt cggctaccgc gaaatcgacc gggcgcagtg
    22081 gcgcctggtg ctggagccga agcagggcat gctcagccat taccgtattg acctcgggat
    22141 caacgccgcc agcgctgctc agctcgccgc gccgtctcgc cttgattctc gctaaccgga
    22201 gccccttatg tcctggaaca accgtgtgat ctggtccgaa ggcatgttca tcggcaccca
    22261 gcatttccag cagcacgacc gctatctgga ggcgctgatc gatgcgcgta gccgccccct
    22321 gtcagcggct ggctggggct tcgccgatct gcagctcgat atggggctac tggcacaagg
    22381 caagctggcc atcgtcagcg cccgtggcct gttacccgac ggcacgccgt tcaatatccc
    22441 gcaggacgac gtggcgccag agccgctgga aatccccgag gacctgcgcg acggcgtgat
    22501 ctacctggcc ctgccgctgc gccgggcagg tgtgcgtgac accgtcgaag cgggtgaagc
    22561 gctgagtggc gcgcgctacc gcggccaggt gcgtgaagta cgtgacgaca acgccgcgtt
    22621 cgaaagccgt gtgccggtga tggtcggcga acgcgctcta cgcctgctga gcgccgccga
    22681 tggcttgcag gaatatgccg cactgggcgt ggcccgagtc cgcgaaaaac gcgccgaccg
    22741 ggccctggtg ctggacgaac agtacattgc gccactgctc gacgtggcag caagtgcgcc
    22801 gctggccgcc tttcgtggcg agctgctggg gctgctgcat cagcgcggcg aagccctcgc
    22861 cgggcgcgtg gtggcctcca gcgccggcgg cgcctcggag attgccgact tcatgctgct
    22921 gcagctggtc aaccgcgccc agccgctgtt cgcccacctg gcccagctgc aaccgttgca
    22981 cccggagcgt ctgtacagcg agctactggc cctggccggt gagttcgcca ccttctctag
    23041 ctccggccgg cgcccgtcgg agttcccgac ctacctgcac gacgacttgc agaccagctt
    23101 cactccggtg atgcaggcgc tgcgcgaagc actgtcgatg ctgatcgaca gcaaggccgt
    23161 gcccattccc atcgtcgaga aggcctatgg cgtgcacgtg gccatgctgg ccgaccgcag
    23221 cctactggac aacgccagtt tcgtcctggt ggtgcgcgcc gacatgcccg ccgaaaccct
    23281 gcgcggacgc ttcggtcagc agagcaagat cggctcggtc gaacacattc gcaatctggt
    23341 caacctgcag ttgcctggga tcaacctgct gccgctgccg gtggcgccgc ggcagattcc
    23401 gttccacgcc ggctcgacct attacgagct cgaccgcggc agcgagcact ggaagcagtt
    23461 ggcacattcc ggcggcttcg cgttccacgt ggcgggtgaa taccccggcc tgaacctggc
    23521 cttctgggcg atacggggtt aatccatgac ggatcaagac gactggctga acaagccgcc
    23581 agcagcacaa ccgcagaaca acgatcgcac ccagttcatg ccgcgcccgg gtggtcgtgg
    23641 cccacagccg cccgtcgagg caccggctgc cccactgcag atgccggccg ccccgctacc
    23701 ggcgggccag gcccgtggta tgaacccact ggagcaggcc gccggcccgc tgctgagcat
    23761 gctcacccgc ctgcgcagca ccctcgccca tcccgcaccg gccagcctgc gtgcgcagtt
    23821 gctgggctac ctgcgccagt tcgaacaacg cgccgaagcc gccggtgtgc cgcgcaacga
    23881 tgtgctgctg gcgcgctacg cgctgtgcac tgccctcgac gaggcggtgc tgagcacgcc
    23941 ctggggcagc accagcgaat ggggcaagca gagcctgctg atcaccgtgc acaacgaggc
    24001 ctggggcggc gagaaggtgt tccagctgct cgaacactgc ctgcaaagcc cgcgcgaacg
    24061 ccttgacctg ctggagctgc tgtatctgtg caccagcctc ggcttcgagg gccgctaccg
    24121 ggtcatgcac gatggccgca gccagctgga ggcgctgcgc gagcgcaccg ccgcgaccct
    24181 gcacagcgtg cgtggcgagc gcgaacacga gctgtcgccg cactggcgcg gagtcagcgt
    24241 ggcgccggat cgcttgtcgc gcctgctgcc gccctgggtc ggtctggccg tggcgctggc
    24301 gttgctgctg gtactgctgc tggtactgcg gatgaaactg gcggccgatg cggagccggt
    24361 gttccgccag atccacgccc tgggcgaagt gccggtgcag agcatcgagc cggcagtggt
    24421 caagccgcgc gtgatcgagc ggccgcgcct ggccaccttc ctcgccgacg acatccgcgc
    24481 caaccgcgtg gcggtcgagg atggcgtcga ccgttcggtg gtgaccatcc gtggcgacga
    24541 gctgttcagc tccggtagcg ccagcatccg tgacgacttc cagccattgc tgctgcgcat
    24601 cgccgaggcg gtcgccaagg tcaagggcca ggtgcgcatc accgggcaca gcgacaacca
    24661 gccgatcgcc acgttgcgct tcccctccaa ctgggcgctg tcgcgcaccc gcgccgagga
    24721 agtgctgcag attctcgcgg cgcgtactgg cccggccgag cgcttcagcg ccgagggctt
    24781 gagcgacacc gaaccgctgg catccaatgc cacggccgaa ggacgggcga aaaatcgccg
    24841 ggtagagata accgtgctgg cggagggtgt ggagtgaagg cgctgttcgg attcatcacc
    24901 cgctgggtca tccctgtgct cgggctgatt gccctgagcc tgatcatctg gttcatcggc
    24961 ccgctgctca acgtgctgca gccggcctgg acgcgctgga cgttgatcat cgtactgttc
    25021 gccctgtggc tcggctggcg cctgttctgt ctgctgcgcg aacgccagca ggagcgcaag
    25081 gtgctcgaca gcctggccgc gcaaacgccg ccggacgccg ccagcgtggc gaccgccgag
    25141 gagctggcga ccctgcgcca gcgcatggac gaagccctgg tgctgctgaa aaaggcgcgt
    25201 ctgggcggcg atgagcgacg caacctgtat cagctgccgt ggtacgtgat catcggcccg
    25261 ccgggctccg gcaagaccac cgcgctggtc aactcggggc tgaatttccc cctcgcggcc
    25321 caactgggcg ccggcgccat tcgtggtgtg ggcggcacac gcaattgcga ctggtggttc
    25381 accgacgaag ccgtgctgct cgacaccgcc gggcgctata ccacccagga cagccatgcc
    25441 caggtagaca aggccgcctg gctcggcttt ctcgagctgc tcaagaacca gcgcgcacgc
    25501 cgcccgatcg atggtgcctt catcgccatc agcctgtccg acctgctgca aagcagcgac
    25561 agcgagcgcg ccgcgcacgt ggcggcgatc cgctcgcggg tgcaggaact gtacagccag
    25621 ctcggcgtgc gcttcccggt ctacgtcatg ctcaccaagt gcgacctggt gccgggcttc
    25681 atggaattct tcgatgacct cagccgtgag gagcgtgccc aggtctgggg ctttaccttc
    25741 gccctggaca acggcaagag catcggcggc ccgctggaaa gtttcgacag cgagttcgtc
    25801 gccctcgagc aacgcctgaa tcagcgtctg atcgagcgtc tgcagcgcga acgcgacccg
    25861 gcccgacgcg acctaatcta cggtttcccc cagcagttcg ccgcgatcaa gagcctgctt
    25921 ggcgaatttc tcaatggcgt gttcaaaccc aatgcctatg aagaacgccc tctgctgcgc
    25981 ggcctgtact tcaccagtgg cacccaggaa ggcagcccga tcgaccgtct gatcggcgcc
    26041 atggcgcaga gcatgaacct cgaccgtcag cacctggccc ggcaaagtgg cagtggtcgc
    26101 agctacttcc tccagcacct gttcagccgc gtggcattcg ccgaacgcgg cctggtcggc
    26161 gtcgaccaga aggtcaaacg gcgccgtagg tggctggcca tcggctcact ggcagccagt
    26221 gtggcgctgg tactggtggt gggcacacta tggctgctca gcttcaacgc caaccgtgag
    26281 catatcgccc aggttggcgc ccgcaccgcc ccactggagc agcaggtgcg cagcctcagc
    26341 ccggcccagc gcgatgtgct ggccgtgctg ccgttgctca gcgccgtacg caacctggcc
    26401 ggcagcccgc cggactgggc caagggcctg ggtctgtatc agggcgacat gctcgaagag
    26461 caggcagcca gcgtctatcg ccgtctgctg gtggccacct tcgccccacg cctggttagc
    26521 cgaatcgagg aacagctgcg cacgggcggc ggctccgact acctctacga agggctcaag
    26581 gcctacctga tgttcggtgg cggcgagcag tacgacgccg agttcatcac ggcctggatc
    26641 gccctggact gggatcagag cctgccgcgc gatctatcgg gcgagcaacg ccaggccctc
    26701 gatgagcacc tgcaggcgct gttcgagcgg cgcccgccag atgcgcgcct ggatcagcgc
    26761 ttgatcgacg agctgcgcat gcagctgcag cgcctgcccc tggcgcagcg tgtgtatgac
    26821 cgggtcaagc ggcagaagct gccggccggg gtcagcgact tccgcctcag cgaggccgcc
    26881 gggcgtgacg ccgcactggt cttcacgcgc aagagcggca aggcgctgag cgacccgctg
    26941 agcggtttct acacccgccg tggctaccgc gaagccttcc tccgtgccag cgtcggccag
    27001 gccagcgtgg tggccgagga acaatgggtg ctgggcagtc agctcgacag cacacaggat
    27061 gccgtgaacc tggccgccga tgtgcgccag ctgtacctgc gcgattacgt gcaacagtgg
    27121 gatgcgctgc tggccgatct cgacttcgtg ccgatcacca gcgtggcgca ggccgccgac
    27181 gtgctgcgga tcctctcggg accttcctcg ccgctcaagc agctgttgat cgcggtggca
    27241 caggagaccg acctgcagca ggcacccgag tccgaaggcg tggccaaggc aaccgacgag
    27301 ggtgtcgagc aactcaagca gaaactcggt tcgctgctcg gccagaccca ggccggtaac
    27361 accgtggctg cggtcacgca gatcgatccg gtcagcgcgc acttcgccga actcaacagc
    27421 ctggtcagcc aggacccatc ggcgccgatg gccatcgatg gtctgctggc ggacctcaac
    27481 gagctctacg tgcaactcag cagcatggtc ggtgccagcg gcgaggcgct gctcggcgag
    27541 gcgaagaatc aggccagtgc agccgccgcg cgggtcaatc tgaccgccgc gcgccagccg
    27601 aagctggtcc aggggctggt gggttcggtc gtcgccgcca gcaccaacag catgatgggc
    27661 ggcgtgcgca accagctcaa cgctgcctgg caaagcgatg tggtcaacgt ctggcggcag
    27721 tcgctgagcg gccgttaccc gatggtggcg ggcagccagc gcgacgccac gctggatgat
    27781 ttcggccagt tcttcggggt cggcggggtc atggacagct actttcgcaa gtacctgcag
    27841 ccctacgtcg acacctcggc acgcaactgg cgctggcagc cgggcgccgc gcagaagctg
    27901 ggcatctccg ccggcgtgct gcagaccttc cagcgtgcgg cgctgatccg cgacgagttc
    27961 ttccgcagcg gcggcctgca gcccagcgtg cgcttcgacc tcaagccagt gagcatggat
    28021 gccaccatca gccagtcgca gctcgatctc gacggccagc tgatcggctt cgaccacggc
    28081 ccgagccggc cggtcgcggt gcaatggccg aaccccggca gcgtcggcgt ggttcgcctc
    28141 acggtgtcgc caccggtcga aggcggacgc tcggcgttga ccctggaagg cccatgggcc
    28201 tggttccgcc tgctcgatca gtccgagctg attggcggcg cctcaccgga tcgcttcaac
    28261 ctgcgcgtgc gcctggatcg ctccagcgtt gcctacgaac tgcgtgccag cagtgcgttc
    28321 aatcctttca agagtcgcgc agtggccggc ttcaccctgc cggagcggct atgacgatga
    28381 tgggtttcta cggcaaggtc gccagccgcg gcgacttcat cgaacgcaac ctgcccaagg
    28441 acttccagcg cggctgggac gcctggctgt cggctggcct gcagaccagc cagggccaac
    28501 tcggcgaaag ctggctggaa gcctatctga ccagcccgct gtggcgcttc gctctggcat
    28561 ctggagtgtg cggtagcgag gccatggccg gggtgctgat gccaagcatc gaccgcgtcg
    28621 gccgttactt cccgctgacg gtcgcctgcc gtattcccgg cgggcagcca ctacaggcct
    28681 tgcaggcgag cgaagcatgg ttcgagcgga tcgaggagct gctgctgagc actctggagg
    28741 aaggcgcccg cttcgaagac ttcgagacga cggtgctgag cctggaaccc ccgcccgcta
    28801 cgacactgcc cgcatacgac caggcgcatg gtctgcagca ttggccggtg gtcgatgccc
    28861 cggggcgtct ggcggcactc gccgcccagg cctgcaacgg tgccagtctg tggtgggggc
    28921 gtggctcgca acgcatcgca gccggattgc gacgctgcca ggggctgccg ccgaccgagt
    28981 ccttcgcggc ttatctgctc gggccggagg tcgtcgcgca atgactgcac tgcattacca
    29041 gtcggcggcc tacagtcatg tcggcctggt tcgccagatc aacgaagacg cctacctgga
    29101 gcgtcccgaa gcgggccttt gggtcgtagc cgatggcatg ggcgggcacg cggccggcga
    29161 ttacgtcagc agtttgatcg tcgacagcct gcgcaacatc aaggcaagcg acaccctgag
    29221 tgactacgag catgttctgc ggctcaccct gagccaggtc aacaccgccg tgcgcgagca
    29281 aacccacctg cgcggcgtga ccatgatggg cagcaccgtg gtgctgatgg tgacccgtgg
    29341 cgaccagggt ttgtgccttt gggccggtga cagccgcctg taccgattga gggcgggtga
    29401 gctgcaggcg gtttcccgtg accacagcta tgtgcaggac ctgcaggaca gcggcctgct
    29461 cagcgaggcc gaggcgcgtc tgcacccacg cgccaacatc gtcacccgcg cggtgggcgt
    29521 gcaggaccaa ctggagctcg cctgccgcgt cttcgacatc cagcccggcg acacctggct
    29581 gctgtgcagc gacggcctta cccgcacggc cgaagaccac gaaatccgcg atgtgctgac
    29641 gcacagcgac ccctatcagg tagtgcgcag cctggtccac ctgggcctga cacgcggcgc
    29701 acccgacaac atcaccgcca tcgtcatcaa ggcgagcgag cccgtctcat ggacctgaat
    29761 attcccggtt tcgacatcga caggatgatt ggcgaaggcg ccatggccac cgtctacctg
    29821 gcgcagcaac gttccctcga gcgcaaggtg gcattgaaga taatggcccc ggcgctggcg
    29881 gccgacgcct cgttctgcga gcgcttcctg cgtgagggca agaccctggc gcgtctggcg
    29941 catccgcata tcgtcaccgt gcacgacatc ggcaatgccg gccagcacta ctacatggcc
    30001 atggagtacc tgcccaacgg taccctgagt gagcgtatcg ccgccggcct gagccccgag
    30061 cagggcttgc agtacatccg ccagatcgcc tcggcgctgg gttatgccca cagccagggg
    30121 ctgatccacc gcgacgtgaa gccagccaac atcctctttc gcgccgacgg cacggcggtg
    30181 ctgtcggact tcggcatcgc caagtcactc gatgacagta cccagttcac ccaggtcggc
    30241 tttgccgttg gcacgcccaa ctacatgagc cccgagcagg cgcgcggtct ggatatcgac
    30301 ggccgcgccg acctttatgc gctgggcgtg gtgctctacg aaatactggt cggcgagctg
    30361 ccgtacatgg gcaccgacgc gctgtccacg gcactggcgc acctgactca gccgttgcca
    30421 gagctgccgc tgcagcacag ccgttatcag accctgctgc aacgcctact ggcgaagaac
    30481 ccggatgagc gtttcaacga tgccggggaa ctgctggccg cgctcaatgc ccagccggca
    30541 ccggactcgg atgcgaccgt gatccgtccg gcgttcaccc agcagaccgc gctgggcgcg
    30601 ccaacgacta gcgagctggg ggagctgcaa ccggtatcca tcgagattcc acgcaatgcg
    30661 ccggcgccga ccgtggcacc cgcaccgact gcccctgcca gcagcgcagc ggcaacgccg
    30721 tccactggca gcaagggccc acgcatggcg ctgctggcgg ttgccgtggc cgccgcgctg
    30781 ggcctgggtg gcggcgccta ctggtggctc agccaaccat ccacagtggt ggctgcggat
    30841 agctcgccgc acagcgacgg gcaaggcgca gctgtcgcgc cgctggctgc cgacaccggc
    30901 gaggccggcg agcatccgct gctgatgccg ggcaagacaa ccatctatca gcgcatcctg
    30961 gccaagcctg gggcgcagct gttgagcgct ccgggggctg ccgctggcga agcattgccg
    31021 gccttctcgg tactctacgt gtatgcccgg cgcagccagg acggccaatc ctggttgcag
    31081 gtcggtgcag ccagcgatgg cagggtgcaa ggctgggtcg ccgccgagca ggccgccgac
    31141 tggaaacaga gcctggtact caagttcacc gagcgctccg ggcgggcacc ggtgctgttc
    31201 atgcagcagg cctcggacgt ggagcgcctg atcgaccgca ctagcctggc tcgcgagcaa
    31261 ctgctgcagg cgcagaacga ccccgagcag gtgccgcagg tggtggcact ggagccgacg
    31321 agcagtgcgg ttccgcagga ccagttctac ctgatgccga tcttcgactt ccgcgaagcc
    31381 ttcgatgccg aaggccagcc ggtgcagctg ctcaacatcg cctcgatcga ccccggcagc
    31441 gccgcccaga gccctgccga ccaaccggct gccgccaccg actcgggttt ccgtaccggc
    31501 atcgtcctgg tggtcgatac ctcggtctcg atgcagccct atatcgaccg cgtgcgccag
    31561 gtggtcagcg agctgcagag ccagctgcag gcgcgcggtg agctggataa cgtcagcttc
    31621 ggcctggtcg gctatcgcaa cagcacggcg cgcacaccgg gcctgcagta cctgagcaag
    31681 accctggtca gcctgcagga aggcagcgat ccacagcgct ttctgcgcgc cgccgagcag
    31741 gtgcaggcaa ccacggtatc cagccattcc ttcaatgagg atgccttcgc cggtgtcatg
    31801 caggccgtcg agggcatgga ctggtcgagc tatggcggcc gcctgatcct gctggtcagc
    31861 gatgccggcg cactgcgcaa gaacgacccc ttcagcagca ccttgatgaa cgaggccgag
    31921 gtgcgtcagg cggcactgag caagcagatc aagatcttcg ccctgcacct gcgcacccct
    31981 gccggcaagg ccaaccacgc ctcggcggag cagcagtatc gggtgctgac ggccgacagc
    32041 aacccgcaga tcggcgacct ctacgtctcg gtgccgggcg gcgatgtcca ggccttcggc
    32101 gagcgcgtgc gcgagatcgg ctcgaccttc gccgaactgg cgcagcaggt tcgcgcgcag
    32161 caggcgccag tacccccaca gctgtccggt ggcaccaacc tggcgaccaa gtcgcaggcc
    32221 atcggctatg ccatgcacat ggatttcctc ggccgccgca gcgccagtca ggcgccgcag
    32281 ctggtcagcg cctggaccgc cgaccgcgac ctgagcaacc cgagcctgcc gacgctgcag
    32341 gtctgcgtga tgctgaccaa gctgcagctc aacgacctgc agcagtcgct caagctgatc
    32401 gtcgacgccg cgcgcaagac caagacctca cccgccgact tcttcagcga gatcgccagc
    32461 gccagtgcct acatgagtcg tgacccagcg gcgctgcgcc agggcgccaa cctggcgcaa
    32521 agcggtgtgc tcggcgagta cctcgacggc ctgccgtatc gcagtaaggc actgagcatg
    32581 agccaggacc tctggctgtc gctgtcggtg gccgagcagg aggacttcat cgacgaactg
    32641 gagtcgaaga ttcgcctcta cgaaaccttc cacaacgaca tggccaactg ggtgcgcttc
    32701 ggcaatgccg agccgggcga cgccctgtac cgtgtaccgc tgtcgacgct gccatgatga
    32761 ttcgcctgca gcaggtcctc aagcaacgtg gccagggcgc gcagcgctac agcctggcca
    32821 tcgagcagtt gcagctggag gccggcgcgc aggtggcact ggtgggcccc agcggctgcg
    32881 gcaagagcac cttgctcgat ctgttggccc tggtgctggc accggacgcg gccgagacct
    32941 tcgacttgga gctgaccggt actgtcagcg atatcgccgc attgtggcag cagggccggc
    33001 agaaccagct ggcccagctg cgcagccaat atctgggtta cgtgctgcag gcgggcgggc
    33061 tgctcggctt tctcgacgtg cgcggcaata tcgcgctgcc ccggcaattg ctcgggatgg
    33121 cagacgacgg ccaggtactg cgtctggccc aggcgctgga tgtgcaggag cagctggata
    33181 aataccccgc gcagttgtcg atcggccaac gccaacgggt cagctgtgct cgcgccctgg
    33241 ctcacgggcc gcagctgctg ctggccgacg aacccactgc ggcgctggac ccgctcaatg
    33301 ccgagcgggt catgcagctg ctgctcgctc aggccgaggc gaccggtgcg acctgcgtgg
    33361 tggcgactca cgacgaggcg ctggcacaac gcgccggcct gaccatcctg cgcatgagct
    33421 gccggcgtga cgccgacggc ggtgtcagcg ctcatctgga gcaggccgcc tgatgcgccc
    33481 gctgctgatc gccggcttgg cctggcagga ctatcgccgc gaggcgcgtc tgtcggcgtg
    33541 cagcgtgctg gcactggtgg cggtgatcgc accattgctg gtgctgtttg gcctgaagta
    33601 cggcctggtc ggcagcctta ccgaacgcct gcaacgtgat cctgcggtgc gcgagatcat
    33661 cccgctgggc ggcacacgcc tgcaggcaga cttcatcgaa gctcttggcc aacgcgaaga
    33721 tgtcgccttc gccatccccc gtacccgaca gatcgccgct accagcgagt tgcaggtgac
    33781 cccaggcgag gcccggttga atgttgagat gctgccgacg gcggtcggcg atcccttgtt
    33841 gcagggcctg ccgatgccgc agacgctgca gcaggtggtg ctcagccata ccgctgccga
    33901 gaagctggga atcaccccag gtagcccgtt gcaggcacgc ttcggccgcc agcacggcag
    33961 tgtgctgcag tggcagcaga ccaccctgca ggtgctggcg gtgctgccgc tggcggcctt
    34021 cgagcgcgac gcgctgttcg cgcaggcggc gctgctggag gccgtcgagg attaccgcga
    34081 cggcctggcg gtggctgcac tggactggcc gggcgcgacg gcaacacaag cgccacgcat
    34141 ctatcccggt tttcgtctgt acgcgcgcgc gctggaggac gtcgagccgt tgcgccagta
    34201 cctggccgcg cagcaggtgc aggtcgtcac tcaagcagcg cagatcgccc aggtgcaggc
    34261 actgagccgc aatctagacc tgctgttctg gctgatcacc accctggctc tggggggcgc
    34321 ggtggcggcc accagtgccg gcgccctggc cgccgtcgag cgcaagcaac gtgagctggc
    34381 ggtgctgcgc ctgctgggcg ttggcagccg cgctttgctg ctgtttctga tcatccaggc
    34441 gctgtacagc ggcctgttcg ccagcgtcct gtcagccctg ctgtaccgac tggcagaggg
    34501 cgggatcaac caccttttcg tgcaggcgcc gggcgattac gccagtcgtc tgctgcccga
    34561 gcactacctg gtggcccttg ctgccgttct gctggccagc gcccttgccg ctgcgctggg
    34621 cggccggcgc gtggcccgga tcgacgcctc tcaaggaatt cgtgatgtct gacagtcgtt
    34681 tcgctccctc tgccctgata ctcgcgacgc tcgcgctggc ggcctgctcg ccagccgatg
    34741 agccaccagc ggccagcact cccgccacac ctgcgcagtc cgaggccgag gcgccacgcg
    34801 aactcgatgc agagcaattg gccagactgg acaaccccaa acccttgccg ggcgacgtca
    34861 gcctgccgct gccctgtggc ggcgaaatgc tcttccgcca tgtctatgtc ctcaccgagg
    34921 gcagcctgga tgaccgcgaa gtcagcctgg gctatccgtt cagcgaaggt gaggccggct
    34981 acaagcaatc cttcatctcc ggctatcgcc gcgacttcat caacggccag ttcagcctgc
    35041 aggacctggc gccgcagtgg cagaagcaga tcgccgaacg cctgcccaag atcgataccg
    35101 gcagcccgct gaaaccgatg atgtatttca tcggcaagta cgaggtcact gcccgtcagt
    35161 acgctcaggt catgacccag gctcaggcac tggccagcgg tgaaacggcg ccggactgtg
    35221 cgccggtcac cagcgcggca gggcggctgc cgcaggtcaa actgtcgcgc ttcgaggctg
    35281 agcgttttgc cgcggtctac agcgcctggc tgctggccaa taaccgtgat ctgctccccg
    35341 tgagcggcag aggcagcaag gcagaggaag gcggcgcggc gttcgtccgt ctgcccaccg
    35401 aggtggaatg ggagttcgcc gcacggggcg gctcgcaggt cagtcgtcag gagctggaag
    35461 cgcgactgtt cccgcgcccg atcgagggca gtgacgatga cggccccctg gctaactggg
    35521 ccgtgttcaa ccaggtcgca ggtggcaccg gtcaggcgcc tcgcctgatg cccatcggca
    35581 ccaagcaggc caacccgctg gggctgttcg acgtgatcgg caacgctgcc gagatggtgc
    35641 aggagtcctt ccagttggtc agtgccgggc gccgccaggg cacctatggc ggcttcgtgg
    35701 tcaagggcgg caactatctg gagggcgagg gcacgctgtt taccggcatg cgccgcgagt
    35761 acccattgtt cggtgcggat ggcagcgagc agcgtaatga gaccaccggc tttcgcgtgg
    35821 cgatcggcgc cttgtccgcc ccgcgttcac gctacgacga gctgttcgcc caatggcaga
    35881 aacagggacg cctggctggc ctgaccgatg acatcgacgc tgccgatgac ccgacccaac
    35941 gcctggacag catcatcgcc agcgccatcg acccgcgcct gcaggctgag ctcggagtgg
    36001 tcaatgaaga gctcaagcgc aacgtcgcgc tgatcgcccg ccagcgcgag gaggccgcgg
    36061 gcaacctgat ccagtctgcc gccctggtgg cggaaaccat caacaactac aacatccgcc
    36121 tgaccaacct gcgccaaagc caacgccagg cgcagcagaa caatgatgct gcctcggcgc
    36181 agatattcgc cggcgccatc gcgaacggca ccaaggcgct caacggcgct gtggccatct
    36241 acatcgacaa catggccagc ggtacccgtt acaccaacgc ggtgattcag gcgcagttcc
    36301 agcgcatcaa ggaagagctc gcgcgcaagc ccgtgatcgg taacagcctg gtcagccgcg
    36361 ccaccttgtt cgtcaatcac gtcgcgctgt accgcactca acaacgcgcc gatccggcgg
    36421 aaatcctcaa gcagttgctg gaaacgcaag cggcggcgca gccgtgagcg tgatcagccg
    36481 ttccacaaac atcaatccac caccagcaaa gagagcctag ctatgcgtcc atccaaaacg
    36541 aacctcacct cgcaacctcg tagccgcgtt ctgacactcg ccgctatcgg ttttacctcg
    36601 atgaccgtac tgatcagcgg ctgctccacc tccgccgtca atcgtgtcgg taaatccacc
    36661 gaggtcgcct attacccaag ttgctaccaa cccgttcagc acctgcgtga taccgactcc
    36721 aacatgactc gctcggtggc taccggcgcc gtgctcggtg cggtcggcgg cgccctcacc
    36781 ggcgcgttga ccgccgacaa ggaagatcgc aagcgcaacg ctgccatcgg cgccgccggt
    36841 ggtgccctgg tcggcggtgc agcggcctac tacacgtcca agcagcagca gatcagcgac
    36901 gaccgtcagc gcatcggctc ctactccacc gacatcaatg ccagtgccaa caccttcgac
    36961 cgcagcatcg cctacgccga agcctcgcag agctgctacc agaaggagtt caccagcctg
    37021 ctgtcgcagc gtaaatccgg tgcgatcaac gatgcagaag ggcgcaagcg tctggccgaa
    37081 atcattgccg gcctgaacga gtccaacaac ctgatcaccg ccgtcaacgg caaggccggt
    37141 gagaacctga gcaactacac ccaggcttac gaggctgacc tgaagaacgt cggcatcgaa
    37201 cgcgccgaag tgaccaccgt cgccaaggcc cctgccaaca agccggtcaa gcagaccaag
    37261 gtgcccaccg aagccgtcag taccgaacgc gttctgcaac aggccgaagc caagcagacc
    37321 aagagccagc aggtagctgt agcaggtagc aaccaggtca acagcatgtg cagcaacccg
    37381 gatatgggcg actgggcacc gattccctgc ccgaacgtct gagttcttgc ggatgattga
    37441 ctgaccgcac gctccgctag tccgcccttt acgggcggac tagcggttat tggagtttct
    37501 accaaggagg gcaaatgtcc gactacctga tcgggttgag aaacacccct tcacagatca
    37561 tggcgcggca gcgcatcgaa gccatgaacg acctgcgcaa actctacaca gccatcgatg
    37621 ccgaccccgc tatctccggc gcaggggtgg tgtacatcga tgccaatttc aacgtggtga
    37681 ccctgcgcga gttcaccccg atctgcagta tcaggcccaa gcgcatcatc attcgtgaag
    37741 cccagcgcaa tgtctcgccg cagcagttcg tccaacaggt gcagtccaac ccgcgcgaat
    37801 cgcggctggt ggccgaaagc gtcaacacca gcctggcctg tgccggcgcg gtactgggct
    37861 ggatcgcgat catcggcggc acggccatcg tgccgttcac cggtggggcg agtctggtga
    37921 tcacgacact gggcgtagca gccgcatcgg cgagctccgt gcaatgtgtg gccggtgctg
    37981 cgcgggtcta taccgaagtg aacaacccgg aagcactcga taggctggac aaccagcagt
    38041 ggtatcagga cgtatccaca gcactggata ttatttctct gggcggcgta gccggggccg
    38101 gagcaacaat tttgcgcgtg atgaacatgc gcagggccat gacagggcgc agctggtatc
    38161 aaagcctgaa tggcctgagc cgtcaggaac gacgtcgttt gaccaacgag atcctggaga
    38221 accgcaaccc ggctctcaca ccggcgcttc gcaaagccct gcagcgtggc ggcgagcttc
    38281 caaaacgctt cagcaccagt gaaatctcgt caacattggc gatcaacatc agagatgcgc
    38341 tgggtgccgg gatcggcttg ctcggtagca gcatggtgca gacttacgcc attggccttt
    38401 acgaggagct agaagagtga accagggact cgatttcctc tcgatattgc gtcgacattt
    38461 cccgatattc atcagtgctt tcttcctggc actgttttct ctggcagtaa gccaaatcct
    38521 gctgctcgag gtctatccgc ccagcctgga agactatggc atctacatga tgatgggcag
    38581 cacaggaata tcgctctacc tgtgcgcagc caaccttttg gtgattcgcg gccgcccctg
    38641 ggcggtatgg ctgatcgtcg cagccatggc ggcctgcata ctggtggtca tcggccactg
    38701 gggcagacgc gcacctgatc tgctgtattt catcggtctg ttgctgccac taagcgccct
    38761 gctgaccctc aacagcaagc gctaccgcac catgctgacg gccatggtgg aagtacgcgg
    38821 tcagcgcacg gcgtggcgca agcgcaacag aaaacggagg tagttgggat actcgaaaaa
    38881 gaccaataac gctaagtcgg gtgccctggt ggctgatggc gctgtttacc ctggtgtgcc
    38941 ttggtggtga tatatggcgg tttgactgaa cagctagcaa agagcggacg ctaaccgaga
    39001 gaagttacag ggatttagac cgcctcaaga ccgagttaaa gatcgctttc ctccaccacc
    39061 ctaaccaccc ccgcttccaa cgcataagcc gcatccgcca actcgttatt gacctgttcg
    39121 atctttagcg tgccgctgac ccagagtggt gcgtagatgt cttccagctc aatgcctttg
    39181 gggtagcgca ccagcaccag ttgattcggc ggtggtgggg ggacgtggat gcaggcgccg
    39241 gggtagggaa ccaggaagaa caacgtgctg cggccggcgg cgtcggtttc cagtggcacc
    39301 gggtagccgc cgagacggat gggtttcccg tcgaaagcgg cgacggtttt gcttgagtac
    39361 atcaccgctg gcaggttctg atcctgctgc ttgaggccgc cggggttgta gaagccgctg
    39421 tcctgttcga ggccctgatg gctgatctcg ggcatgtctt ccagggcctg gcggtcttcc
    39481 ggcggcatca gctcaagcca gtcggtttct ggtagttcgg cgcaggccag ggtgccgata
    39541 aacaacaggg gtaacagcag ataacgcatc ggtaggctca ccaagcagcg gagtgaaagg
    39601 acatcagtcc tttctgacag cggcgtagat taccagcagc accacggcgc cgatcactgc
    39661 gccaataaag ccggcggact cgcccacttt gtagatgccc agtgcctggc cgccataggt
    39721 agcggcaact gagccgccga cgcccagcag gatggtcatg atccagccca tcctgtcatt
    39781 gccgggtttg ataaagcgcg cgatcagacc gacgatcagg ccgataaaaa tgatgccaat
    39841 aatgctcatg cgagcctcct gagtggacgg tgggatttgc gaccgttgat ctgaccacgg
    39901 gaaaagctgg cagttcgcta ctcttcggtc tctggcggcg cgacgccata gcggtggtag
    39961 atgcgggcga tctcgccgct ctcgcgcatg cctcgcagcg cctggcctag ctgctcgtag
    40021 agggcctttt tctcgccccg atagtggcgc atcgacgcac cgtaataatt accgacggat
    40081 tgctcgtact ggtattcggc atagctgtag tcgctaaagc cgatcttgtg gaactgcgcc
    40141 tcgatggctt cggcatccag caccaccatc acatccaggc gcccggcgcg aaacatcccg
    40201 gccagcaagt agtcgtcagt cgccaggctt tttgtcaggg cactgtcacc gtcgaactga
    40261 tcgaaatagg cggtgccgcg tttgaccccg atcaccttgc cctgcagatc ggcatagttg
    40321 cgcaggctgg cctgctgacc ggtcttgacg ataaagcgga tgcttttgcg ctgggtgccg
    40381 atcgacggca gaaagtgcac gtagtccttg cgctcggcgg tgaacagcac gcgcggcacc
    40441 aagtcgatac gtccggactt gaggtcttcc aggctgcgca caaagggcgc gtaacgccat
    40501 tcgatgcgcg cgccgacgcg ttgcgcggcg ctatcaagca cctcgatcag cgggccgacg
    40561 ggaagtgagt cgagggcctg catctctggc gggcgctcgc ggtaatcggc gcgcaacacc
    40621 tggtcagcat ggcaaagcgg caggtacagc aaagacagca gcaacagata cttcatgacg
    40681 aggctcttgt gtatggccta cgcaagagct tagtcacaaa gccggcttca ggccttggcg
    40741 atcagcgcct cgaccacggc gatctgctta ttcagggtga cgcgatccgc acagcgcagg
    40801 gtggcgtgac caaccttgcg gccgaccttg aaggccttgc cgtagtgatg caggcggcag
    40861 tcggcgacgg cgataacctg ctccaccggc ggaaccacac cgataaagtt aagcatcgcg
    40921 ctctcgccga ccttggcagt cgaacccagc ggcaggccgg caacggcgcg caggtggttc
    40981 tcgaactggc tgcactcggc accttcgatg gtccagtgcc cggagttatg cacacgcggg
    41041 gcgatttcgt tggctttcag gccgccatcg acttcgaaga actcgaaggc cagcacgccg
    41101 acgtagtcga gcttggtcag cacgcggctg acgtaatctt ctgccagcgc ctgcagcggg
    41161 tgatcggtgc tggcgatgga cagcgccagc acaccgctgt cgtggcggtt gtgcaccagc
    41221 gggtagaagc gcgtttcgcc atcacgagca cgcaccgcca ccagcgacac ttcgccagtg
    41281 aagggcacga agccctcaag gatgcacggc acgctgccca gctcggcaaa ggcgccgacg
    41341 acatcttctg gcttgcgcag gaccttctgg cccttgccgt cgtagcccag ggtgcgggtc
    41401 ttcatcacgg ccggcaggcc aatgctggcc acagcggcgt cgaggtcagc ctgcgactgg
    41461 atatcggcga aatccggggt gggaatgccg aggtccttga acatggattt ttcgaaccag
    41521 cgatcacgag cgatgcgcag ggcttcggcg ctcgggtaca ccgggacgaa ctgcgagagg
    41581 aaagccacgg tctcggccgg cacactttcg aactcgaagg tcaccagatc gacttcatcg
    41641 gccagctgac gcaggtgatc ctgatcgccg taatcggcgc ggatatgttc ccccagcgct
    41701 tgcgcgcagg catccggcgc ggggtcgagg aaggcgaagt tcatccccag cggcgtgcct
    41761 gccagggcca acatgcggcc cagctggccg ccaccgatta cgccgatctt cattacgcgt
    41821 ccctcgggtc cgggttttcc agtacagatt cagtctgctc agtccggaac gtcttcaggc
    41881 gctcgtggaa ctgcggatgc tggtgaccga gaatgctcgc ggcgagcagt gcggcattga
    41941 ttgcgccagc cttgccgatg gccagggtgg cgaccggaat accggccggc atctgcacga
    42001 tcgacagcag cgagtcgacg cccgaaagca ccgccgattg cacgggtacg ccgagtaccg
    42061 gcaggtgggt cttggccgca cacatgcccg gcaggtgcgc cgcgccgccg gcaccggcga
    42121 tgatcacctg aatgccacgt tcttcagcct gttcggcgta ctggaacagc aggtccgggg
    42181 tgcggtgggc ggaaaccacc ttcacttcgt acggaatgcc tagcttgtcc agcatctcgg
    42241 cggtgtggct aagggtggac caatcggact tggagcccat gatcacgcca accagtgcgc
    42301 tcatcgtcgt gcctcttctt catcaagtgc ccgtaggcgc tgcagaaact aacaagccac
    42361 gc
//
LOCUS       DASRUM010000221         3748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2130295, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000221 DASRUM010000000
VERSION     DASRUM010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2130295"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..180)
                     /locus_tag="VFF22_15055"
                     /pseudo
     CDS             complement(76..180)
                     /locus_tag="VFF22_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            398..1825
                     /gene="leuC"
                     /locus_tag="VFF22_15060"
     CDS             398..1825
                     /gene="leuC"
                     /locus_tag="VFF22_15060"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014852276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HZX18168.1"
                     /translation="MAGKTLYDKLWEMHEVKRRDDGSSLIYIDRHILHEVTSPQAFEG
                     LRLASRKPWRIDANIATPDHNVPTTKAERQGGLEAIADEVSRIQVQTLDENCDDFGIL
                     EFKMNDIRQGIVHVVGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLA
                     TQCLVAKKMKNMKVAVEGQLPFGVTAKDIVLAVIGKIGTAGGNGHALEFTGSAIRDLS
                     LEGRMTICNMSIEAGARVGLVAVDEKTIAYVEGRPFAPKGSDWAAAVAQWQDLVSDAD
                     AHFDTVVELRAEDIKPQVSWGTSPEMVLAVDQNVPDPAAENDPVKKDSITRALKYMGL
                     QANQAITDIQLDRVFIGSCTNSRIEDLRAAAEVAKGRKVASTIKQAMVVPGSGLVKAQ
                     AEKEGLHHIFIEAGFEWREPGCSMCLAMNPDKLGSGEHCASTSNRNFEGRQGAGGRTH
                     LVSPAMAAAAAVTGRFIDVRELIQA"
     gene            1836..2483
                     /gene="leuD"
                     /locus_tag="VFF22_15065"
     CDS             1836..2483
                     /gene="leuD"
                     /locus_tag="VFF22_15065"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HZX18169.1"
                     /translation="MKAFTQHTGLVAPLDRANVDTDQIIPKQFLKSIKRSGFGPNLFD
                     EWRYHDVGQPNQDNSKRPVNQEFVLNFARYQGASVLLARENFGCGSSREHAPWALEEY
                     GFRTIIAPSFADIFFNNSFKNGLLPIILKEDEVDALFQQAEATVGYQLTVDLAAQTVT
                     RPDGVQYSFEVDAFRKHCLLNGLDDIGLTLQDSDAIKTFESTYQQRSPWLFGAIK"
     gene            2564..3646
                     /gene="leuB"
                     /locus_tag="VFF22_15070"
     CDS             2564..3646
                     /gene="leuB"
                     /locus_tag="VFF22_15070"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HZX18170.1"
                     /translation="MSKQILILPGDGIGPEIMAEAVKVLNLANDKYNLGFELSFDDLG
                     GAAIDRYGVPLADETLARAKVADAILLGAVGGPKWDAIDPAIRPERGLLKIRSQLGLF
                     GNLRPAILYPQLADASSLKREIVAGLDILIVRELTGGIYFGQPRESKVLENGERMAYD
                     TLPYSESEIRRIARVGFDMARVRGKKLCSVDKANVLASSQLWRAVVIEVAKDYPDVEL
                     SHMYVDNAAMQLVRAPKQFDVMVTDNMFGDILSDEASMLTGSIGMLPSASLDANNKGM
                     YEPCHGSAPDIAGQGIANPLATILSVSMMLRYSFSQTAAADAIELAVSKVLDQGLRTG
                     DIYSAGTTKVGTAAMGDAVVEALRSL"
BASE COUNT          768 a         1108 c         1149 g          723 t
ORIGIN      
        1 gagcagggcg ccaccttgcc gggcatgacc gttgtctgcg gcgactcgca cacttcgacc
       61 cacggcgcat tcggcgcgct tgctcaccgc tggctgggtc agatgcaggc gctcggccgc
      121 ctccgaaaag ctgcccagct cggcgatggc gataaaggcg ttgagggtgg cgaggtccat
      181 aacgcactag ctccaagtga taagccatag gcagcaagag caaggctgtg gctccttgct
      241 tgcaccttga ggcttacaga ctatcgctat tcccgcaagg aatcctttga ataaaaaata
      301 tgaatttgag taattcgatg gcagcccata ggattgcccc ataagccata gggccattcg
      361 ccccaggcat agaaagatgc tgatgaggaa aacgctgatg gccggcaaaa cgctctacga
      421 caagctctgg gaaatgcatg aggtcaagcg ccgcgacgat ggttcgtcgc tgatctacat
      481 cgaccggcat atcctccacg aagtgacctc gccccaggcc tttgagggct tgcgtctggc
      541 tagccgcaag ccgtggcgca tcgacgccaa catcgccacc ccggaccaca acgtgccgac
      601 caccaaggct gagcgccagg gcggcctgga agctatcgct gatgaagtgt cgcggattca
      661 ggtgcaaacc ctggatgaga actgcgatga cttcggcatc ctcgaattca aaatgaacga
      721 catccgccag ggcatcgtcc acgtggtcgg cccggagcag ggcgccaccc tgccaggcat
      781 gaccgtggtc tgcggcgact cgcacacctc gacccacggc gcctttggcg cgctggccca
      841 cggcatcggc acctcggaag tcgaacatgt actggcgacc cagtgcctgg tggccaagaa
      901 gatgaagaac atgaaggtcg cagtcgaggg ccagctgccg ttcggtgtga ccgccaagga
      961 catcgtcctc gccgtgatcg gcaaaattgg aaccgctggc ggcaatggcc atgccctgga
     1021 gttcaccggc agcgcgatcc gcgacctgtc gctggaaggg cgcatgacca tctgcaacat
     1081 gtccatcgaa gccggcgctc gcgtcggctt ggtggcggtg gatgagaaaa ccatcgccta
     1141 cgtcgaaggc cgtccgttcg cacccaaagg ctctgattgg gccgccgccg tggcgcagtg
     1201 gcaggacctg gtgtccgacg ccgatgctca tttcgacacc gtggttgagc tgcgcgccga
     1261 agacatcaag ccgcaggtca gctggggcac ttcgccggaa atggtgctgg cggtggatca
     1321 gaacgtgccg gacccggccg ccgaaaacga cccagttaag aaggattcga ttacccgcgc
     1381 cctgaaatac atgggattgc aggctaacca ggcgatcacc gatatccagc tggatcgcgt
     1441 gtttatcggc tcctgcacca actcgcggat tgaagacctg cgcgccgccg ctgaagtggc
     1501 caagggccgc aaggtcgcca gtacgatcaa gcaggcgatg gtggtgccgg gttctgggct
     1561 ggtcaaagcc caggcggaaa aagaaggttt gcaccacatc tttatcgaag ccggttttga
     1621 atggcgcgag ccgggttgtt ccatgtgcct ggcgatgaat ccggacaaac tcggcagtgg
     1681 cgagcattgc gcatctacct ccaaccgcaa ctttgaaggc cgtcagggtg ctggtgggcg
     1741 tactcacctg gtcagcccgg caatggctgc tgctgctgcg gtgaccggcc gctttatcga
     1801 tgtgcgcgaa ttgatccagg cctaaggagc tgacgatgaa agcctttact caacacaccg
     1861 gcctggtcgc tccactggat cgcgccaacg tcgacaccga ccagatcatc cccaagcagt
     1921 tcttgaagtc gatcaagcgc agcggtttcg gccccaacct gttcgatgag tggcgttacc
     1981 acgatgttgg tcagcccaat caggacaact ccaagcgccc ggtgaatcag gagttcgtgc
     2041 tgaacttcgc gcgttaccag ggtgctagcg tgctgctggc gcgggagaac tttggttgcg
     2101 gctcatcccg tgagcatgcg ccttgggcgc ttgaggagta cggtttccgc acgatcatcg
     2161 cgccgagctt tgccgatatc ttcttcaaca acagcttcaa gaacggcctt ttgccgatca
     2221 tcctcaagga agacgaggtc gatgcgctgt tccaacaggc ggaagccacg gttggctacc
     2281 agctgaccgt cgacctggcc gcgcaaaccg tgacccgtcc ggatggcgtg cagtacagct
     2341 tcgaggttga tgccttccgc aagcattgcc tgctcaacgg tttggacgat atcggcctga
     2401 cccttcagga cagtgacgcg atcaagacct ttgaaagtac gtatcagcag cgcagtccct
     2461 ggctgtttgg tgcaatcaag taggctgtgc ggctgcacca tggaacaacg acttttgagc
     2521 attatggtgc gcatggcgca ccctacggcg tattgaggat gcaatgagca aacagattct
     2581 gattctcccc ggcgacggta tcggcccgga aatcatggcc gaagcggtca aggtgctgaa
     2641 cctggccaac gacaagtaca acctgggttt cgaactgagc ttcgatgacc tcggtggtgc
     2701 ggccatcgac cgttacggcg tgccgctggc cgatgaaacc ctggcccgcg ccaaggttgc
     2761 cgatgccatt ctgctcggcg ccgtaggcgg cccgaagtgg gacgccattg accctgcgat
     2821 ccgtccagag cgcggcctgt tgaagattcg ctcgcagctg ggcctgttcg gcaacctgcg
     2881 tccggcgatt ctctatccgc aactggccga tgcttccagc ctcaagcgtg aaatcgtcgc
     2941 tggcctggat atcctcatcg tgcgcgagct gaccggcggc atctatttcg gtcagccgcg
     3001 cgaaagcaaa gtgctggaaa acggcgagcg catggcctac gacaccctgc cgtacagcga
     3061 gagtgaaatc cgccgtatcg cccgcgtcgg cttcgacatg gcccgtgtgc gtggcaagaa
     3121 gctctgctcg gtggacaagg ccaacgtgct ggcttccagc caactctggc gcgccgtggt
     3181 tattgaagtg gccaaggatt atccggacgt cgaactcagc cacatgtacg tcgacaacgc
     3241 cgccatgcag ctggtgcgtg cgccgaagca gttcgacgtg atggttaccg acaacatgtt
     3301 cggcgacatc ctttcggatg aagcctcgat gctcactggt tccatcggca tgctgccgtc
     3361 ggcgtctctg gacgccaaca acaagggcat gtacgaaccg tgccacggca gcgcgccgga
     3421 tatcgctggg cagggcattg ccaacccgtt ggcgaccatt ctgtcggtgt caatgatgct
     3481 gcgttacagc ttcagtcaga cggcagcagc cgatgccatt gaactggccg tgagcaaggt
     3541 gctggatcaa ggcctgcgta cgggcgacat ctacagcgcg ggtacgacca aggtcggcac
     3601 cgcggcaatg ggtgatgcgg tagtcgaagc gctgcgtagt ctgtaatctt cctggcccgc
     3661 cggaggtctc cggtggtcag cttatatagg tgtagtggtt atgaaacgtg taggtctgat
     3721 cggttggcgc ggcatggtcg gttccgtg
//
LOCUS       DASRUM010000222        13581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_111521, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000222 DASRUM010000000
VERSION     DASRUM010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 13581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13581
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111521"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VFF22_15075"
     CDS             <1..523
                     /locus_tag="VFF22_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008375223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZX18171.1"
                     /translation="DPNSSKPEDYEKASALMLKIRPHVAYFHSAKYMTDIANGDICVA
                     IGYSGSFYQFANRAKEAGNGVVVDWRLPKEGAPLWYDSFAIPKSAQNVSEAHEFLNNL
                     LDPKVVAPISDFLGYPNPNKDAMPLVGAEIRDNPGLTPTAEAQKTLYVLQPLPQKIER
                     VRTRVWNTIKSGT"
     gene            complement(579..1484)
                     /locus_tag="VFF22_15080"
     CDS             complement(579..1484)
                     /locus_tag="VFF22_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZX18172.1"
                     /translation="MNLESKWLEDFVTLAATRSFSQAAQKRFVTQPAFSRRIRSLENM
                     LGLTLVNRACTPIELTESGQLFLVTARSMVEQLGEVVRHLHNLEGQQGEVMQIAAAHS
                     LTLGFFPEWIARLRREGLPLTTRLVATNVGEAVHALREGACDLILAYYDPDAALQMDP
                     EIFPSLHLGRTEMLPVCAVGEDGAPLFDLNSGQSVPLLAYSAGAFLGRSVNLLLRQRA
                     LRSTTVYETAMADSLKSMALQGMGVAWVPRLSVTAELARGELVVCGDEQWQAPLEIRL
                     YRCALVRKASVRLLWRKLESGEVGR"
     gene            1654..3081
                     /locus_tag="VFF22_15085"
     CDS             1654..3081
                     /locus_tag="VFF22_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HZX18173.1"
                     /translation="MSAAASTRLEKDLLGTLDVPADAYYGIQTLRAVQNFRLSGVTLS
                     HYPKLVIALAMVKQAAADANRELGHLSAAKHTAISTACARIIQGEFHDQFVVDMIQGG
                     AGTSTNMNANEVIANIALEAMGFEKGDYKHLHPNNDVNMAQSTNDAYPTAIRLGLLLG
                     HESLLTALDKLIQSLSKKHLEFGHVLKMGRTQLQDAVPMTLGQEFRAFATTLGEDLQH
                     LKLLAPTLLTEVNLGGTAIGTGINADPQYQLMAVKRLAIISGQPLTPAADLIEATSDM
                     GAFVLFSGMLKRTAVKLSKMCNDLRLLSSGPRTGINEINLPPRQPGSSIMPGKVNPVI
                     PEAVNQVAFEVIGNDLALTMAAEGGQLQLNVMEPLIAYKIFDSIRLLTRAMDMLRELC
                     IDGITANEARCRELMENSIGLITALNPYIGYENATRIAKVALESGRGVLELVREEKLL
                     GDAELADILRPENMIAPRLVPLLPG"
     gene            3240..4493
                     /locus_tag="VFF22_15090"
     CDS             3240..4493
                     /locus_tag="VFF22_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003297763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HZX18174.1"
                     /translation="MSQSSTSSLGFLRRMPLWQQILIGLGLGVLTGVLFKADALVLAP
                     IGTLFLNAIKMLIVPLVFVSLVAGITAMSDSAKLGRISVKTIAIYLITTAFAVSIGLL
                     FGALFTPGEGMNMVASGVQETKQAPSLVQILVGLVPTNPVAAFAEGNILQIIVFAIAL
                     GVSMNLIGEKAAPAVKLFDSLAEVFYKLTDLVMRCAPIGVFALIAGVVASHGIEVLLP
                     LASVIGVIYLASITHMLLVYGGLVSVLARLSPLRFFKGVAPALAVAFSTSSSAGTLPV
                     SIECARKNLGVSQGVAGFVLPVGATINMDGTAIYQGVLALFIAQAFGIDLNAGQYLMI
                     ILTATLASIGTAGVPGAGLIMLGLVLTSVGLPLEGVALIAGIDRILDMARTTVNVAGD
                     LMTTTLVGSSEKELDRDIYNSDNAQ"
     gene            4944..6368
                     /locus_tag="VFF22_15095"
     CDS             4944..6368
                     /locus_tag="VFF22_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HZX18175.1"
                     /translation="MLDILNDLIWSKLLIVMLIGLGLYFTIASRFVQFRYFGSMFRIF
                     AEAFQRQPGQLSSFQALMLSVAGRVGAGNIAGVSVAIMLGGPGAIFWMWVVALVGMAT
                     SYFECSLAQLYKRREDDGTYRGGPAFYIQHGLGQRWLGIVVSILLLVTFGFGFNAVQS
                     FTVASSMHDTFGLPTYASGAILTVVMGLIIFGGIKRIAKFADVLVPVMAFSYIGMALF
                     VIGSNVDAIPATLALIFKSAFGLEPAFAGGIGAAILMGVKRGLFSNEAGLGSAPNVAA
                     VAEVKHPVAQGIVQSLSVFIDTIILCSCTALIILLSGVYQPGTEMAGVVLTQTAVASV
                     VGEWGRVFVTVALLLFVFTTLIYNYYLGENALGFFSKKRGPVVVYRVLVVALVLWGSV
                     QDLGTVFAFADVTMGLLAICNLIALVLLFKVGLRLMRDYDSQIKAGVESPVFDAKNFA
                     DLDLDPAVWQHTPNQPPINAQQQR"
     gene            6475..7230
                     /locus_tag="VFF22_15100"
     CDS             6475..7230
                     /locus_tag="VFF22_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX18176.1"
                     /translation="MRHILCTALLSCLLFSPLSQAQLPPDYKVVLLTENFPPFNMADH
                     GKNFARDANITGISADIVREMFKRAAIDYSLTLRFPWDRIYQQTMTTANHGLFSTSMS
                     EARKPLFKWVGPIAQYQSVLVGLKGSSPQLDNLEQAKAYRVGAYQSAAVSQHLERLGF
                     IPQNALRDQENIRKLQRGQIDLWATSDPVWRFYAKEQGADDLQTLLSFETSQLYLALN
                     KDTPDEVIERLQSALEQMRSEGYGGCAQNPELC"
     gene            complement(7374..8849)
                     /locus_tag="VFF22_15105"
     CDS             complement(7374..8849)
                     /locus_tag="VFF22_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZX18177.1"
                     /translation="MPSVPPLPVDLSGIRLDPQRGLARQLYQALRERILDGRLQRRTR
                     LPASRDLASLLGISRNTVTRAFDQLYAEGYIEGRIGAGTYVAELSGAKRPTPVPPTQP
                     PVSPQSAALQLLQTHHLSPPLSGAPRAFRVGVPAFDLFPFETWARLSARFWRKPSPAR
                     LGYGDPAGDLQLRELIAAYLRNSRGLHCDPAQIVVTNGAQQAISLCAQLLVTPGARVA
                     VENPGYRAAGHALAIAGAQLCGVAVDDQGLDTAALEQLEDCRLVYVTPSHQYPTGVTL
                     SLARRLQLLEWAERNNGLIIEDDYDGEYRYSGTPLAPLAALDRQGRVLYVGTFCKIAF
                     PALRLGYLVLPPALAEAFAQRRALDMRHSEIGTQAVMAEFIAAGHFQRHIRRMRSAAR
                     SRRDALLAGWPDSVPGCAPLPVVEAGLHLCVRVNSLARERELISAAERIGVEISALSS
                     YWLPDSSTPEDQRAGLVLGFAAVPEAQIAEALQALRKAWGI"
     gene            9063..9443
                     /locus_tag="VFF22_15110"
     CDS             9063..9443
                     /locus_tag="VFF22_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZX18178.1"
                     /translation="MYIGKAADLTGCTPKAIRLYESMGLLPAPSRQGRYRLYNAHHVA
                     IIHIIRVAQAAGFKLIEMVPLLKEKERRQVFPLELANQEIAAKRQQVQADIAALTALD
                     ERLVELQAEVNVLFATPVSQACSR"
     gene            complement(9440..10423)
                     /locus_tag="VFF22_15115"
     CDS             complement(9440..10423)
                     /locus_tag="VFF22_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017738934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZX18179.1"
                     /translation="MSKLCLVTGANGHLGNTLVRALLARGDKVRAGVRNLDNRTPFEG
                     LACELVYAELQDKTAMRKALEGVDVLYQVAAVFRHWARDPYEEIILPNINGTRLLLEA
                     AAEAGVRRIVYVSSVAAVGHNGQLLDEERWNDEVGNPYYLSKILSERMAWEVAQKHGL
                     WMVSVLPSAMIGPNADRLTDTMSFLESVQGRRVPLDPNFHFNFVDVRDVAEGMIQAAE
                     RGRSGQRYILANRHSSSLAVLIDAANDIEPGYRLPPLAPRWLLLGLAWLQERLARVTG
                     KPAELLVSQVRLFYGVKQEYSIAKAQAELGYCPRPPEQALKAAFGYLRDRG"
     gene            10732..11382
                     /locus_tag="VFF22_15120"
     CDS             10732..11382
                     /locus_tag="VFF22_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HZX18180.1"
                     /translation="MYTPAAFRQDDLATLHQQIRATRLPTLVSHGTQGLLASHLPLLL
                     EADEGPYGKLYGHFARANPQWRDLAEGSPALVIFQGAEAYISPSWYAAKAEHGKVVPT
                     WNYISVQAHGRAEVFDDAERLLQLVSRLSALHEAERPQPWAVSDAPRDYIDSMLRAIV
                     GFALPIERLEGKWKLGQNRNQADQTGVREGLAASLDPRDRELAAKINNQHLAKEAP"
     gene            11379..11822
                     /locus_tag="VFF22_15125"
     CDS             11379..11822
                     /locus_tag="VFF22_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX18181.1"
                     /translation="MSIEIRPVSTADHAAWLPLWQGYLHFYQSELPAEVSAMTWQRFL
                     DPSEPTHAALAWRDGQAVGLVQWIFHRTNWSVEHSCYLQDLFVGADQRGGGIGRRLIE
                     HVYAQAQAAGCSRVHWLTQETNYPGRQLYDRIAERSGFIQYRKQF"
     gene            11837..12514
                     /locus_tag="VFF22_15130"
     CDS             11837..12514
                     /locus_tag="VFF22_15130"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZX18182.1"
                     /translation="MPQQPLLDWQPVPTPVHQPLDGQYVRLEPLDPAHHGDDLWQALQ
                     GPHSDPALWDYLPYGPFSERAAFDTWLSGQAASRDPLFFSVIDLRQQRAVGLLSFLRI
                     TPKDGCIEIGHIAFGRAMQRSPASTEAVYLLAKLALGELGYRRLEWKCNAANARSMRA
                     AERLGFRYEGLFRQHMVVKGENRDTTWFAILDSEWPACNAAFARWLAADNFDDEGRQQ
                     QRLQDLR"
     gene            complement(12527..13345)
                     /locus_tag="VFF22_15135"
     CDS             complement(12527..13345)
                     /locus_tag="VFF22_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX18183.1"
                     /translation="MSDHLGLNLKLLCSHYRSIAEVCRKLAINRAQFNKYLSGQSRPT
                     AYNLKRICDFFGVEAYELSLPAEQFADLIGARTPGQDAGARTDALMELLKPLRAHAGN
                     LSRYCGYYFEYSNCMSVPGTVLLSLVQLWEEDGDFLFERQERQERSASTDVQGEDWVR
                     CRYLGAAFQLQDRLFLMDYESLTLNEMSQTILIPSYKSRITRLNGLKTGVSSGDRRSP
                     ACTRVVWEYLGTEINRISAYRQVRLYRPDDPRIDDDVRQRLAVAPLKNGLFEIE"
BASE COUNT         2669 a         4486 c         4011 g         2415 t
ORIGIN      
        1 cgaccccaac agcagcaagc ctgaggacta cgaaaaggcc agcgcgctga tgctgaagat
       61 tcgccctcac gtcgcctact tccactccgc caagtacatg accgatatcg ccaacggcga
      121 catctgcgtg gccatcggct actcgggcag cttctaccag ttcgccaacc gcgccaagga
      181 agccggcaac ggcgtggtgg tcgactggcg cctgccgaag gaaggcgcgc cgctgtggta
      241 cgactccttc gccatcccga aaagcgcgca gaacgtcagt gaggcccatg agttcctcaa
      301 caacctgctc gaccccaaag tagttgcgcc gatcagcgac tttctcggtt accccaaccc
      361 gaacaaggac gccatgccac tggtgggcgc agaaatccgc gacaacccgg gcctgacccc
      421 aaccgccgaa gcgcagaaaa ccctctacgt gctgcaaccg ctgccgcaga agattgaacg
      481 cgtacgcacc cgagtgtgga acaccatcaa gtcgggtacc tgatggcagc acggcgcagc
      541 gggcaatccg ctgcgccgac ttattcagcg aaaaggcttt agcggcccac ttcgccgctt
      601 tccagcttgc gccagagcag gcgtaccgag gccttgcgca ccagggcgca gcggtacagg
      661 cggatttcca gaggcgcttg ccactgctca tcgccgcaca ccaccaactc gccacgggcc
      721 agttcggcgg tcacgctcag ccgtggcacc caggccacgc ccatgccctg caaggccatg
      781 cttttcaggc tgtcggccat cgccgtttca tacaccgtgg tcgagcgcag cgcgcgttgg
      841 cgcagcagca gattgaccga gcgaccgaga aaagccccgg cgctgtaggc cagtagcggc
      901 acactctggc cactgttgag gtcgaacagc ggcgcgccgt cttcgcccac cgcacacacc
      961 gggagcattt ccgtacggcc caggtgcagc gaggggaaga tttccgggtc catctgcagc
     1021 gcagcgtccg ggtcgtagta agcgaggatc aggtcgcagg cgccttcacg cagcgcatgc
     1081 accgcttcgc cgacgttggt cgccaccaag cgggtggtca acggcagacc ttcacggcgc
     1141 aggcgggcga tccactcggg gaaaaaaccc agggtgagcg agtgcgcggc agcaatctgc
     1201 atcacctcac cctgctggcc ttcgaggtta tgcaggtgac gcaccacctc gccgagctgc
     1261 tcgaccatgc tccgtgccgt gaccagaaac aactggccgg attcggtcag ctcgatgggc
     1321 gtacaggcgc ggttgaccag cgtcaggccg agcatgttct ccaggctgcg gatgcgccga
     1381 ctaaacgccg gctgggtaac aaagcgcttt tgcgccgcct gggagaagct gcgggtggcg
     1441 gccagggtga caaagtcttc cagccatttg ctttcgaggt tcatggatca gcccttgtgc
     1501 atgcgaacac gtcggtcgac gttgagcggt cacgccaacg ttatgccgat tatgcatgac
     1561 ccagcgctta acagcattgg ccagtatcaa gctgcagccc ctaacctacg ggccatcgcg
     1621 gcatatgccg tatttcctga gatgaccttg atcatgtccg ctgctgcatc cactcgcctc
     1681 gaaaaagacc tgctcggcac cctcgacgtt cctgctgatg cctattacgg catccagacc
     1741 ctgcgcgccg tgcagaactt ccgcctgtcc ggcgtgacgc tgtcgcacta cccgaaactg
     1801 gtgatcgcgc tggccatggt caagcaggcc gctgccgacg ccaaccgcga gctgggccat
     1861 ctgtccgccg ccaagcacac ggcgatcagc accgcgtgcg cacgcatcat tcagggcgag
     1921 ttccacgacc agttcgtggt cgacatgatt cagggcgggg ccggcacctc gaccaatatg
     1981 aatgccaacg aagtcatcgc caatattgcc ctcgaagcca tgggtttcga gaagggtgac
     2041 tacaagcacc tgcacccgaa caacgacgtg aacatggcgc agtcgaccaa cgacgcctac
     2101 cccaccgcca tccgcctggg cctgctgctc ggccacgaga gcctgctgac agcgctggac
     2161 aagctgattc agtcgctgtc gaaaaaacac ctggagttcg gccacgtgct gaagatgggc
     2221 cgcacccagc tgcaggacgc cgtaccgatg accctgggtc aagaattccg cgccttcgcc
     2281 accacccttg gcgaagacct gcagcacctc aagctgctcg ccccgaccct gctcaccgaa
     2341 gtgaacctgg gcggcaccgc gattggcacc gggatcaacg ccgacccgca gtaccagctg
     2401 atggcggtca agcgcctggc gattatcagc ggccagccgc tgaccccggc cgctgacctg
     2461 atcgaagcca cgtcggatat gggcgccttt gtactgttct ccggcatgct caagcgcacc
     2521 gcggtgaagc tgtcgaagat gtgcaacgac ctgcgcctgc tgtccagcgg cccacgcacc
     2581 gggatcaacg agatcaacct gccgccgcgc cagcccggca gctcgatcat gcccggcaag
     2641 gtcaacccgg taatcccgga agcggtcaat caggtggcct tcgaagtgat cggcaacgat
     2701 ctggcgctga ccatggcagc cgaaggcggc cagctgcagt tgaacgtgat ggagccgctg
     2761 atcgcctaca agatcttcga ctcgatccgc ctgctgacgc gcgccatgga tatgctgcgc
     2821 gagctgtgca tcgacggcat caccgccaac gaagcgcgct gccgtgaact gatggaaaac
     2881 tccatcggcc tgatcaccgc actcaacccc tatatcggct acgaaaacgc cacgcgcatc
     2941 gccaaggtgg cgctggaaag tggtcgcggc gtactggagc tggtgcgcga ggagaagctg
     3001 ctgggcgacg ccgagctggc tgacatcctg cgccccgaga acatgatcgc cccgcgcctg
     3061 gtaccgctgc tgccgggctg atcccgaaaa aaccctacgc gccgtctccg caaggaacgg
     3121 cgcgtttttt tcaccagcac ccagaccatg ggtagctggt tttccgccct cgcctgccgg
     3181 gcggcaccgc tcaaggccgc acagacggcc ccacaaaaac gataattcgg gtgataacca
     3241 tgagtcagag cagtacttct tccctaggct tcctgcggcg catgccgctg tggcagcaaa
     3301 tcctcatcgg cctcggcctc ggcgtgctca ccggcgtgct gttcaaggcc gacgccctgg
     3361 tgctggcacc catcggcacg ctgttcctca acgcgatcaa gatgctgatc gtgccgctgg
     3421 tgttcgtctc cctggtggcc ggcatcaccg ccatgagcga cagcgccaag ctcggccgca
     3481 tcagcgtcaa gactattgcc atctacctga tcaccaccgc ctttgcggtg agcatcggcc
     3541 tgctgttcgg cgccctgttc accccgggtg aaggcatgaa catggtggcc agcggcgtac
     3601 aggaaaccaa gcaggcaccg tcgctggtgc agattctggt cggcctggta ccgaccaatc
     3661 cggtggccgc ttttgccgaa ggcaatatcc tgcagatcat cgtgttcgcc atcgccctgg
     3721 gcgtgagcat gaacctgatc ggcgaaaagg ccgcaccggc agtcaagctg ttcgacagcc
     3781 tggccgaggt gttctacaag ctcaccgacc tggtgatgcg ctgcgcgccg atcggcgtat
     3841 ttgcgctgat cgccggcgtc gtggcctccc acggcatcga agtgctgctg ccgttggcga
     3901 gcgtaatcgg ggtgatctac ctggccagca tcacccatat gctgctggtg tatggcggcc
     3961 tggtcagcgt gctggcgcgg ctcagtccgc tgcgtttctt caagggtgtg gcgccggcat
     4021 tggccgtggc cttcagcacc tcaagcagcg ccggcaccct gccggtgtcg attgagtgcg
     4081 cgcgcaaaaa cctcggcgtg tcccagggcg tggccggctt cgtgctgccg gtgggcgcca
     4141 ccatcaatat ggacggcacc gccatctacc agggcgtact ggcgctgttt atcgcccagg
     4201 ccttcggtat tgatctgaat gccggccagt acctgatgat catcctcacc gccaccctgg
     4261 cctcgatcgg caccgctggc gtgcccggcg ccgggctgat catgctcggc ctggtgctga
     4321 cctcggtcgg cctgccgctg gaaggcgtgg cgctgatcgc cggtatcgac cgcatcctcg
     4381 acatggcgcg caccacggtg aacgtcgccg gcgacctgat gaccaccacc ctggtgggca
     4441 gcagtgagaa ggaactggat cgcgacatct acaacagcga caacgcgcaa taacgcggcg
     4501 ctgctcacgg gcaacacccg cacagggacg tgcggggctt ttgcgccaac ccggcgcagc
     4561 ggcattgccg gcaggacccg gcagccaacg agatgcctat cggcatccag ggcataccaa
     4621 taaaaacaac cacacagggt gacctcatga acgcattcga ccccggtcgt ctctctccgc
     4681 caggcagcag gccacccccg ctgccctagt gcctgaatag cccgttcagt cggctcggcg
     4741 gcgaatcctc ctgcggcggt ttatggcatg ccatcagcca tcgcagggtt tctgtgtcgg
     4801 cgcacaaccc tcgtcaccga ccgactgcac aggctcccag cacatggaac acccgtgccg
     4861 tccgccagcg tcgccctacc cgggcggcgc tgcggcgtca tcatcattaa tcccccgata
     4921 aatacaaaaa agggagagac cccatgctcg atattctcaa tgacctgatc tggagcaagc
     4981 tgctgatcgt gatgctgatc ggccttggtc tgtacttcac catcgcctcg cgtttcgttc
     5041 agttccgtta cttcggcagc atgttccgca tcttcgccga agccttccag cgtcagccgg
     5101 gtcagctcag ctcgttccag gcgctgatgc tctccgtcgc cgggcgcgtt ggtgccggca
     5161 acatcgccgg tgtctcggtg gcgatcatgc tcggcggccc gggcgcgatc ttctggatgt
     5221 gggtcgtggc gctggtcggc atggccacca gctacttcga atgttccctg gcgcagctat
     5281 acaagcgccg tgaagacgac ggcacctacc gcggtggccc ggccttctac atccagcacg
     5341 gcctcggcca gcgctggttg ggcatcgtcg tatcgatcct gctactggtg accttcggct
     5401 tcggcttcaa cgccgtgcag tcgttcaccg tggccagctc gatgcatgac accttcggtc
     5461 tgccgaccta tgccagcggc gcgattctga ccgtggtaat ggggctgatt atcttcggtg
     5521 gcatcaagcg tatcgccaag tttgctgacg tgctggtgcc agtgatggcc ttctcctaca
     5581 tcggcatggc gctgttcgtg atcggcagca acgttgacgc tatcccggcc accctggcgc
     5641 tgatcttcaa gagcgcgttc ggtctggaac ctgcctttgc cggcggtatc ggcgcggcca
     5701 tcctgatggg cgtgaagcgc ggcctgttct ccaacgaagc gggcttgggc agcgcgccga
     5761 acgtcgctgc agtggccgag gtcaagcacc cggtcgccca gggcatcgtg caatcgctca
     5821 gcgtatttat cgacaccatc atcctgtgca gctgcaccgc actgatcatc ctgctgtccg
     5881 gcgtgtatca gccaggtacg gaaatggccg gcgtggtgct gacccagacc gctgtcgcca
     5941 gcgtggtcgg tgagtggggt cgggtgttcg ttaccgtggc gctgctgctg tttgtgttca
     6001 ccaccctgat ctacaactac tacctcggcg aaaacgccct gggcttcttc agcaagaagc
     6061 gcggcccggt ggtggtctac cgggtgctgg tcgtggcgct ggtcctctgg ggttcggtgc
     6121 aggatctggg tacggtgttc gccttcgccg acgtgaccat gggtctgttg gccatctgca
     6181 acctgatcgc tctggtgctg ctgttcaagg tcggcctgcg tctgatgcgc gactacgaca
     6241 gccagatcaa ggcgggcgta gaatcgcctg tattcgacgc gaagaacttc gccgatctgg
     6301 atctcgaccc tgctgtctgg cagcacacgc caaaccagcc cccgatcaac gcgcaacaac
     6361 agcgttgatc tgaatacgcc caggcgcttg gcctgggcgt tgttgtttat gtcataggcc
     6421 ccgcgcggcc agttggcagg cgcgggactc tccgtacaag tggagtgcca gaccatgcgt
     6481 catattcttt gcaccgcgct gttgtcctgc ctgctgttca gccccctgag ccaggcgcag
     6541 ttgccacctg actacaaggt ggtgctgctg acggaaaact tcccgccctt caacatggcc
     6601 gaccacggca agaacttcgc gcgcgacgcc aatatcaccg gcatcagcgc cgacatcgtg
     6661 cgcgagatgt tcaagcgcgc cgccatcgac tacagcctga ccctgcgctt cccctgggat
     6721 cgcatctacc aacagaccat gaccacggcc aaccacggcc tgttttccac ctccatgagc
     6781 gaggcacgca agccgctgtt caagtgggtg gggcccatcg cccagtacca aagcgtgctg
     6841 gtcggcctca agggcagcag ccctcaactg gacaacctgg aacaggccaa ggcctatcgc
     6901 gtcggcgcct accagagcgc ggcagtcagc caacacttgg agcgcctggg ctttattccg
     6961 cagaacgccc tgcgcgacca ggaaaatatc cgcaaactgc aacgcgggca gatcgacctg
     7021 tgggccacct ccgacccagt ctggcgcttc tatgccaaag aacagggcgc cgatgatctg
     7081 caaaccctgc tcagcttcga aacctcgcag ctatacctgg cgctaaacaa ggacaccccg
     7141 gacgaggtaa tcgaacggct gcaaagcgcc ctggagcaga tgcgcagtga gggctatggc
     7201 ggctgcgcgc aaaaccccga gctgtgctga atcgggccac ttgcgtgctg ccgcgcagtt
     7261 gctcaagccg ctgcgactga cctatgtagg gtgcgccatg cgcaccactg acaaccgggt
     7321 atctggtgcg cacggcgcac cctacagacc ggcgttatcg aaacaatatt ctgctagata
     7381 ccccaggcct tgcgtagcgc ctgcaaggcc tcggcgatct gcgcttcggg caccgcagca
     7441 aaacccagca ccagcccggc gcgctgatct tccggcgtgc tgctgtcggg tagccagtaa
     7501 ctgctgagtg cacttatctc cacgccaatc cgttcagcag cgctgatcag ctcgcgctcg
     7561 cgggccaggc tgttcacccg cacgcataga tgcagcccgg cctccactac gggcagaggc
     7621 gcacaacccg gcaccgagtc aggccaacca gccaatagtg catcgcgacg actgcgcgcc
     7681 gcgctgcgca tacggcggat atgccgctga aaatgcccgg cggcgataaa ttcggccatc
     7741 accgcctgcg tgcctatttc cgaatggcgc atatccagag cgcgacgctg ggcaaaggct
     7801 tcggccagcg ctggcggcaa taccagataa cccagacgca acgccggaaa ggcgatcttg
     7861 caaaaggtgc cgacatacag cacccgtccc tggcggtcga gggcggccag gggtgccagg
     7921 ggcgtaccgc tgtagcggta ctcgccgtcg tagtcgtcct cgataatcag gccattattg
     7981 cgctcggccc attccaataa ctgcagacgc cgcgccagcg acagggtgac gccggtcggg
     8041 tactggtgcg acggcgtcac atagaccaac cgacaatctt caagctgctc cagcgcggcg
     8101 gtgtccagac cttggtcatc caccgccacc ccgcacagct gcgctccggc tatcgccagg
     8161 gcatggccgg cggcgcggta gccggggttc tccacggcca cccgcgcgcc gggcgtgacc
     8221 agcaactgcg cgcacaggct gatggcctgc tgcgcgccat tggtaacgac gatctgcgcc
     8281 ggatcgcagt gcaggccacg gctgttgcgc aagtaagcgg cgatcagctc gcgcaattgc
     8341 aagtcgccgg ccgggtcgcc atagcccagg cgtgccggcg acggcttgcg ccaaaagcgc
     8401 gcagacaacc gcgcccaggt ctcgaaggga aacagatcaa aggccggtac accgacgcga
     8461 aacgcccggg gggcgccgct taatggtggg ctcagatgat gcgtctgcaa cagctgcagg
     8521 gcggcacttt gcgggctgac gggcggctgc gtcgggggca ccggagttgg ccgcttcgcc
     8581 ccgctcaact cagccacata agtgccggcc ccgatgcgcc cctcgatata gccctcggcg
     8641 tacagctgat cgaaggcgcg ggtcacagtg ttgcgcgaga tgcccagcag gctggccaga
     8701 tcgcgactgg ccggcaaacg cgtgcggcgt tgcaggcgac catcgaggat gcgctcacgc
     8761 agcgcctgat acagctggcg cgccagaccg cgttgcggat caaggcgaat gccggacaaa
     8821 tcgaccggga gcggcggaac agacggaatg gccatgcaaa ttggctccac taaagctaca
     8881 gaagtcgatc ttacaaaaga ccaatacctt ttctagcatg actccaaacc caggaggtgg
     8941 actaggcaca cgccagccgc ttactgacgc tgatcagccc ggctaccagc ccgcgctatc
     9001 atccaatatg ggcacccact agaatccgct gccctggtct taacaaggaa cggaactcca
     9061 ctatgtacat tggaaaagct gctgatctga cgggctgtac gccgaaagcc atacgtcttt
     9121 acgagagcat gggcttactg ccggcaccca gcaggcaggg gcgctatcgc ctctataacg
     9181 cgcaccatgt agccataatc cacattatcc gagtcgccca agcggcaggt ttcaagctca
     9241 tcgagatggt gccattgctc aaggagaagg aacgacggca ggttttcccc cttgagctgg
     9301 ccaaccagga aattgccgcc aagcgccagc aggtgcaggc agatatcgcg gcgcttacag
     9361 cgctggacga gcgcctagtg gagctccagg cggaggttaa cgtattgttt gccacgcctg
     9421 tttcccaggc ctgcagtcgc tagcctcgat cacgtagata gccaaaggca gccttcaacg
     9481 cctgctccgg cggtctcggg caataaccca gttcggcttg cgccttggca atgctgtact
     9541 cctgcttgac gccatagaac agccgcacct ggctaaccag cagctctgcg ggtttaccag
     9601 tcacccgcgc cagtcgttcc tgcaaccagg ccaaacccag cagcagccag cggggagcta
     9661 acggaggtag gcggtagcct ggttcgatgt cattggcggc gtcgattaat actgccagcg
     9721 aggacgaatg tcgatttgcg aggatgtagc gttgaccgga gcgccccctc tctgccgcct
     9781 ggatcatgcc ctcagcaaca tcgcgtacat ccacaaagtt gaagtggaag ttcgggtcga
     9841 gcggcactct gcgaccctga accgactcta gaaagctcat cgtatcggta aggcgatccg
     9901 cgttggggcc gatcatggcc gagggtaaaa ccgaaaccat ccacagacca tgcttctgcg
     9961 ctacctccca ggccatacgc tctgacagga tcttggacag gtagtaaggg ttgcccacct
    10021 catcattcca gcgctcttca tccagcaact ggccgttatg cccaacggcg gcaaccgaac
    10081 tgacgtaaac gatgcgcctg acaccggcct cggctgcggc ctccagcaac aagcgggtgc
    10141 cgttgatatt gggcagaata atttcctcgt acggatctct ggcccaatgc ctgaatacgg
    10201 ccgcgacctg atagagcaca tccaccccct ccaacgcctt gcgcatggcc gtcttgtcct
    10261 gcagttccgc atagaccagc tcgcaagcca ggccctcgaa aggcgtgcgg ttatcgaggt
    10321 tgcgcacgcc agctcgcacc ttgtcaccgc gcgccagcag cgccctgacc aacgtgtttc
    10381 cgagatgccc gttggctcct gtcaccagac ataatttgct catcgtggct cgttaccctt
    10441 gcagactgac tatggataga tgcgctcagg gtaaagtctg tccctcggga cagagtcaag
    10501 cctcgatgaa ggtgaattag gtagctggcg tccacgacag gcaaccaaga ggggtggcgg
    10561 acaacacttg gctcggcgac gtatgcgcca tcacccatac acccgacata aaccgagcga
    10621 agacgcgggt ggagtaggca tggaaattgg ctctataagg ttcgcagaaa ttggatctta
    10681 caacagacca atactctgcc tagcatggtg catcagctca ggagcctacc catgtataca
    10741 cccgccgcct tccgccagga cgacctcgcc accctgcacc agcagatacg cgcaacacgc
    10801 ctgccgacgc tggtcagcca cggcactcag ggcctgctcg ccagccatct gccgctgctg
    10861 ctggaggccg acgaagggcc ttacggcaag ctgtacggcc acttcgccag ggccaacccg
    10921 cagtggcgtg acctcgccga aggcagcccg gcgctagtga ttttccaagg agcggaggcc
    10981 tacatcagcc cgtcctggta cgccgccaag gccgagcacg gcaaggtggt gccaacctgg
    11041 aattacatca gcgtgcaggc ccatggccgc gccgaagtgt tcgatgacgc cgagcgcctg
    11101 ctgcagctgg tcagccgcct cagcgcgctg cacgaagccg agcgcccgca accctgggcg
    11161 gtcagcgatg caccgcgcga ctacatcgac agcatgctgc gcgcgattgt cggcttcgcc
    11221 ctgcccatcg aacgcctgga gggcaaatgg aagctcgggc agaaccgcaa ccaggccgac
    11281 caaaccggcg tgcgcgaagg tctggctgcc agcctagacc cacgcgaccg cgagctggcc
    11341 gcgaagatca ataaccaaca cttagccaag gaagcgccat gagcattgaa attcgccccg
    11401 tcagcactgc cgatcacgcc gcctggttgc cgctatggca gggctatctg cacttttacc
    11461 agagcgagtt gcccgccgag gtcagcgcca tgacctggca gcgctttctc gacccaagcg
    11521 agcccaccca tgccgccctc gcctggcgcg acggtcaggc cgtggggttg gtgcagtgga
    11581 tcttccaccg cactaattgg tcggtggaac acagctgcta cctgcaggat ctgtttgtcg
    11641 gcgccgatca gcgaggcggc ggcatcggtc ggcggctgat tgagcacgtc tatgcccaag
    11701 cccaggcggc tggctgcagc cgcgtgcatt ggctgaccca ggaaaccaac tacccaggcc
    11761 gccagctcta cgaccgcatc gccgagcgct cgggctttat ccaataccgc aaacagttct
    11821 gaacaggagc cgcagcatgc cccagcaacc actactcgac tggcagcccg tgcctacacc
    11881 tgtccatcaa cccttggacg gccagtatgt gcgcctcgaa ccgctcgacc cggcgcacca
    11941 tggcgacgac ctgtggcagg cgttgcaagg cccgcacagc gacccggcac tttgggatta
    12001 cctgccttac ggcccattca gtgagcgcgc agcgtttgat acctggctaa gcggccaggc
    12061 cgccagccgc gacccgctgt ttttcagcgt gatcgaccta cgccagcagc gcgccgtcgg
    12121 cctgctgagc tttctgcgca tcacgcccaa ggatggctgc atcgaaatcg gtcatatcgc
    12181 cttcggccgc gccatgcagc gcagcccggc gtcgaccgaa gcggtgtatc tgctggccaa
    12241 gctggccttg ggcgagcttg gttaccgccg cctggaatgg aaatgcaacg ccgccaatgc
    12301 ccgttccatg cgcgcggctg agcgtttggg cttccgctac gaagggctgt ttcgccagca
    12361 catggtggtc aagggagaga accgcgacac cacctggttt gccatcctcg acagcgaatg
    12421 gccggcctgc aacgcggcct tcgcgcgctg gctggcggcg gacaacttcg atgatgaggg
    12481 acggcagcag cagcgtctgc aagacctgcg gtaatagcgc agcggtttat tcgatctcga
    12541 acagcccgtt cttcagcggc gccacagcca ggcgctgacg cacatcatcg tcgatgcgcg
    12601 gatcatccgg gcggtacagg cgcacctggc ggtaggcgct gatacggttg atctcggtgc
    12661 ccaggtattc ccacaccacg cgggtacaag ccgggctgcg tcgatcaccg ctggagacgc
    12721 cggttttcaa gccgttaagg cgagtgatgc ggctcttgta gctgggaatc aggatggtct
    12781 gactcatctc gttgagggtc agcgactcgt agtccatcag aaacaaccgg tcctgcagct
    12841 ggaacgcagc gcccaggtag cggcagcgca cccaatcctc gccttgcaca tcggtactcg
    12901 cggaacgctc ttggcgctcc tgacgctcga acaggaagtc accgtcttcc tcccacagct
    12961 gcaccagcga caacagcaca gtgcccggca ccgacatgca gttggagtac tcgaagtagt
    13021 agccgcaata gcgcgacagg ttgccagcat gcgcgcgcag cggcttgagc aactccatca
    13081 gcgcgtcggt gcgcgcgccg gcatcctggc ccggcgtgcg cgcaccaatc aggtcggcaa
    13141 actgctcggc cggcaggctt aattcatagg cctcgacgcc gaaaaaatcg cagatgcgct
    13201 tgagattgta ggccgtcggt cggctctgtc cgctcaggta tttgttgaac tgggcgcgat
    13261 tgatcgcgag cttgcggcaa acctcggcga tcgaccggta atggctgcac aacagcttga
    13321 gattgagccc tagatggtcg gacataactg atttcctggt gatgcgagcg acgcgattct
    13381 agcatcaact cgcatcaggt cgccgcgacc tgcgaaattg cacgacgaac gcgtctgctc
    13441 aaccattcgg acccgcccat attcccttca caaaacaaca agagagacct cactgccatg
    13501 ctcgatattc tcaacgacct gatctggagc aagctcctga tcgtgatgct gatcggcctt
    13561 ggtctgtact tcaccatcgc c
//
LOCUS       DASRUM010000223         2980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1777968, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000223 DASRUM010000000
VERSION     DASRUM010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777968"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VFF22_15140"
     CDS             <1..963
                     /locus_tag="VFF22_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZX18184.1"
                     /translation="DSLGADILRLWVSSTDYSGEMAVSKVILQRSADSYRRIRNTARF
                     LLSNLSGFDPAQHLLPAEDMLALDRWAVDRALLLQREIEEAYDSYRFWNVYQKVHNFC
                     VQELGGFYLDIIKDRQYTTAADSVARRSCQSALFHIAEALVRWIAPVLSFTADELWEY
                     LPGARNESVMLNGWYQGLSELPQDFELSREYWERVMAVKVAVNKEMEGLRAAKAIGGN
                     LQAEVTLYAEDSLIADLNKLSNELRFVLITSTAALAPLADAPADAVVTEVAGLKLKIV
                     KSAHPKCGRCWHHREDVGVNPAHPEICGRCVDNIEGAGEVRHYA"
     gene            1048..1551
                     /gene="lspA"
                     /locus_tag="VFF22_15145"
     CDS             1048..1551
                     /gene="lspA"
                     /locus_tag="VFF22_15145"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZX18185.1"
                     /translation="MSARFGKLPWLWLSLLVFVVDQASKFYFDNSLSMYQQIVIIPDY
                     FSWTLAYNTGAAFSFLADAAGWQRWFFAAIAVAVSAVLVVWLKRLKPGETWLAVALAL
                     VLGGALGNLVDRVVFGHVVDFILLHWQHRWYFPAFNVADMAITGGAVLLALDMFKSEK
                     SGEKAHD"
     gene            1544..1981
                     /locus_tag="VFF22_15150"
     CDS             1544..1981
                     /locus_tag="VFF22_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZX18186.1"
                     /translation="MTDVRIGPDKEVTLHFAIKLENGDVVDSTFDKKPATFKVGDGNL
                     LPGFEQAIYGLKAGDKRSLPIGPEQGFGQGNPQNIQIMPRSQFQDMELSDGLLVIFND
                     AANAELPGVVKAFDDSQVTIDFNHPLAGKTLSFDVEIIEVKAL"
     gene            2110..>2980
                     /gene="ispH"
                     /locus_tag="VFF22_15155"
                     /gene_synonym="lytB"
     CDS             2110..>2980
                     /gene="ispH"
                     /locus_tag="VFF22_15155"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010451823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HZX18187.1"
                     /translation="MHIKLANPRGFCAGVDRAIEIVNRALEVFGPPIYVRHEVVHNKF
                     VVEDLRSRGAIFVEELDQIPDDVIVIFSAHGVSQAVRNEAERRGLKIFDATCPLVTKV
                     HMEVVRYSREGRECILIGHAGHPEVEGTMGQYDASNGGAIYLVEDEADVARLQVRNPE
                     ALAFVTQTTLSMDDTSKVIDALRGKFPSIGGPRKDDICYATQNRQDAVKQLAAECDVL
                     LVVGSHNSSNSNRLRELAERMGTPAYLIDGAEDLKREWFDGVTGIGITAGASAPEVLV
                     RGVVSQLQAWGATG"
BASE COUNT          569 a          909 c          919 g          583 t
ORIGIN      
        1 gacagcctgg gcgccgatat cctgcgcctg tgggtgtcgt ccaccgacta ttctggcgag
       61 atggcggtgt ctaaggtcat cctgcagcgc agcgccgatt cctaccggcg tatccgcaac
      121 accgcacgct tcctgctctc caacctcagc ggcttcgacc cggcgcagca cctactgccg
      181 gctgaagaca tgctggcgct ggatcgttgg gccgtagatc gcgccctgtt gctgcagcgc
      241 gaaatcgaag aagcctacga cagctaccgc ttctggaacg tgtaccagaa ggtgcacaac
      301 ttctgcgtgc aggagctggg tggcttctac ctcgacatca tcaaggaccg tcagtacacc
      361 accgccgctg atagcgtggc gcgccggtcc tgtcagtccg cactgttcca tatcgccgaa
      421 gccctggtgc gctggatcgc accggtgctt tcgtttaccg ctgatgagct gtgggaatac
      481 ctgccgggcg cgcgcaacga gtcggtgatg ctcaatggct ggtatcaggg cctgagcgag
      541 ctgccgcagg actttgagct gagccgcgaa tactgggagc gggtcatggc ggtcaaggtt
      601 gcggtgaaca aagagatgga aggcctgcgc gcggccaagg ccatcggcgg caacctgcag
      661 gcggaagtca ccctgtatgc cgaagacagt ctgattgccg acctgaacaa gctcagtaat
      721 gaattgcgtt ttgtgctgat cacctccacc gctgcgctgg cacctttggc cgatgcgccg
      781 gccgatgctg tggtcaccga ggtggcgggt ctgaagctga aaatcgtcaa gtctgctcac
      841 cccaagtgcg gccgctgctg gcaccaccgc gaagacgttg gggttaaccc ggcgcatccg
      901 gaaatctgcg ggcgctgcgt ggacaatatc gaaggcgcgg gcgaggttcg tcactatgca
      961 tgatgcgctt ttgctcccct cgtcctccgg gagaggggcc gggcgtgagg gtctctggct
     1021 cgcatccgaa cctaaggatg tctgcttatg agcgcccgtt tcggcaagct gccctggctc
     1081 tggctgagtc tgctggtgtt cgtggtcgat caggcgagca agttctattt tgacaacagc
     1141 ctgagcatgt accagcagat cgtgatcatt cccgattact tcagctggac cctggcctac
     1201 aacaccggcg cagccttcag ctttctcgcc gatgcggccg gctggcagcg ctggttcttc
     1261 gcggccattg ccgtagcggt cagcgcggtg ttggtggtct ggctcaagcg cctcaagcca
     1321 ggcgagacct ggttggcagt ggctttggcc ttggtgctgg gcggcgcgct gggtaacttg
     1381 gtcgatcgcg tggtgtttgg ccatgtggtc gactttattc tgctgcactg gcagcaccgc
     1441 tggtatttcc ctgcgttcaa tgtcgccgat atggcgatta ccggcggcgc ggttttgctg
     1501 gcgctggata tgttcaaaag cgagaagtcc ggagaaaaag cccatgactg acgtacgtat
     1561 cggcccggac aaggaagtca ccctgcactt cgccatcaag ctggaaaatg gcgacgtggt
     1621 cgacagcacc ttcgataaaa agcccgccac cttcaaagtg ggcgatggca acctgctgcc
     1681 gggtttcgag caggccatct atggtctgaa agccggcgac aagcgcagcc tgccgattgg
     1741 ccccgaacag ggcttcggtc aaggcaatcc gcagaatatt cagatcatgc cgcgcagcca
     1801 gtttcaggac atggaactgt ctgacggctt gctggtgatc ttcaatgatg ccgccaatgc
     1861 cgagctgccc ggcgtagtaa aagccttcga cgacagccaa gtaaccatcg acttcaacca
     1921 cccgctggcc ggcaagacgc tgagtttcga tgtggaaatt attgaggtta aagcgctctg
     1981 atctgtagcc cggataaaat ccgggaaacg cgaatgcccc tcccggattc cgtccgggct
     2041 acgcaaagcg ggagcgctgt ctaatcccgc gttaaacttg cccctgaacg accgcgtgtc
     2101 gaggctctca tgcatatcaa actcgccaat ccccgcggct tctgcgccgg cgtcgaccgc
     2161 gccattgaga tcgtcaaccg tgcgctggaa gtcttcggcc ctccgatcta tgtgcgccat
     2221 gaagtggtgc ataacaagtt tgtggtcgag gatctgcgtt cgcgtggcgc gattttcgtc
     2281 gaagaactag atcaaattcc tgacgacgtt atcgttatct tcagcgccca cggcgtctcc
     2341 caggctgtgc gcaacgaagc cgaacgccgt ggcctaaaga tcttcgacgc cacctgcccg
     2401 ctggtgacca aggtgcacat ggaagtggtg cgctacagcc gcgaagggcg cgaatgcatc
     2461 ctcatcggcc atgccggcca cccggaagtc gagggcacca tgggccagta cgacgccagc
     2521 aacggcggcg ccatctacct ggtggaagac gaggccgatg tcgcgcgcct gcaggtgcgc
     2581 aaccccgagg ccctggcctt cgtcacccag accaccctgt cgatggacga caccagcaag
     2641 gtcatcgacg ccctgcgcgg caaattcccc agcatcggcg ggccgcgcaa ggacgacatc
     2701 tgctacgcca cccagaaccg ccaggacgcg gtcaagcaac tggctgccga gtgcgacgtg
     2761 ttgctggtgg tcggcagtca caacagctcc aactccaacc gtttgcgcga gctggccgag
     2821 cgcatgggca ctccggccta cctgatcgac ggcgccgaag acctcaagcg cgagtggttc
     2881 gacggcgtta ccggcatcgg catcaccgcc ggcgcctcgg cgccggaggt gctggtgcgt
     2941 ggcgtagtca gccaattgca agcctggggc gcgacaggcg
//
LOCUS       DASRUM010000224         2559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1299928, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000224 DASRUM010000000
VERSION     DASRUM010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299928"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFF22_15160"
     CDS             <1..517
                     /locus_tag="VFF22_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZX18188.1"
                     /translation="RIGTRIGIMEGGKLVQVGTPQELIDNPANDYVRNFFNTVDTNRY
                     LTAGQLMADSVPIYVHNGKAPDAQKVCQELQALDKHYAFIVDEQHTFQGSISLERIAL
                     LVEGGTTCGLDDQVLKSIKPIPEDLPLDQVIERLVDNEGPIPVVDSNGRYSGAISKGR
                     LLTRLQGEFHE"
     gene            510..1391
                     /locus_tag="VFF22_15165"
     CDS             510..1391
                     /locus_tag="VFF22_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     permease"
                     /protein_id="HZX18189.1"
                     /translation="MSEKQLDLGSWVNDVVQHLLDNYSEGFDSIGAVVNGFSEGIEWL
                     LMLPPAWLLIAIFVGLGLWRISYKFAIFTAVAFVLIVLTGFWEQTVVTLGLTFSATLI
                     SLLLGIPLGIWAARSERVATIIRPILDFMQTMPAFVYLIPAAMLFGLGRVPGIIATVI
                     FAMPPAVRLTNLGIRQVNKEIIEAGQSFGCTPRQLLFKVQLPNAMPSIMAGVNQTIMM
                     ALSMVIIASMVGAGGLGNDVLASIQRLDIGLGFESGMAVVLLAIILDRITESFGTPQT
                     AKRSSLFGWFSGKLQRQ"
     gene            1424..2368
                     /locus_tag="VFF22_15170"
     CDS             1424..2368
                     /locus_tag="VFF22_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline ABC transporter substrate-binding
                     protein"
                     /protein_id="HZX18190.1"
                     /translation="MKTIKQLAGVGLLSVAMLQSAWAQEPESCKMVRFAEIGWADIAA
                     TTGVAMTLSEGLGYQTRKIMASVPIAFTGVQKGQIDVFLGYWAPSMDAVIEPFTKDGG
                     VKVLPKANLEGAKYTLAVPTYAAEAGLKSFADIAKFKDQLGGKIFGIEPGNDGNLLIE
                     GMIKSNQFDLGDFKMIESSEAGMLVQLQRAVKKKEPAVFLGWAPHPMNTQFDITYLEG
                     GDDVFGPDFGAAKVYTVVPPDYEARCANVGKLLNNLQFTVEIESQLMEKVLDKQNPTT
                     VAKEWIKANPAILDQWLAGVTTYDGQDGVAAVKKHLGM"
BASE COUNT          553 a          834 c          729 g          443 t
ORIGIN      
        1 gcgcatcggc acccgcatcg gcatcatgga aggcggcaaa ctggttcagg tcggcacccc
       61 gcaggagctg atcgacaacc ccgccaacga ttacgtgcgc aacttcttca acaccgtcga
      121 caccaaccgc tacctcaccg caggccagct gatggccgac agcgtgccga tctacgtgca
      181 caacggcaaa gccccggatg cgcagaaggt ctgccaggag ttgcaggcgc tggacaagca
      241 ctacgccttt atcgtcgacg agcagcacac cttccagggt tccatcagcc tggagcgcat
      301 cgctctgctg gtcgagggcg gcaccacctg cggcctcgac gaccaggtgc tcaagtcaat
      361 caaaccgatc cccgaagacc tgccgctgga ccaggtgatt gaacgcctgg tggacaacga
      421 agggccgatc ccggtggtcg acagcaatgg ccgctacagc ggcgccatca gcaaaggtcg
      481 cctgctgacc cgcctgcagg gagaattcca tgagtgagaa acagctcgac ctgggcagct
      541 gggtcaacga cgtggtgcag cacctgctgg acaactacag cgaaggcttt gacagcatcg
      601 gcgcggtggt caacggcttc tccgaaggca tcgaatggct gctgatgctg ccaccggcct
      661 ggctgctgat cgccatcttc gtcggcctgg gcctatggcg catcagctac aagttcgcca
      721 tcttcaccgc cgtggccttt gtgctgatcg tgctcaccgg cttctgggaa cagaccgtgg
      781 tgacgctcgg cctgaccttc tcagccacgc tgatcagttt gctactcggc ataccccttg
      841 gcatctgggc ggcgcgcagc gagcgagtgg cgaccatcat ccggccaatt ctcgacttta
      901 tgcagaccat gccggcgttc gtttacctga ttcccgccgc catgctcttc ggcctcggcc
      961 gtgtgccggg gatcatcgcc acggtgatct tcgccatgcc accggcggtg cgcctgacca
     1021 acctcggcat ccgccaggtc aacaaggaaa tcatcgaagc tggccagtcc ttcggctgta
     1081 ccccgcgtca gctactgttc aaggtccagc tgcccaacgc catgccgtcg atcatggccg
     1141 gggttaacca gaccatcatg atggccctgt cgatggtgat catcgcctcg atggtcggcg
     1201 caggcggcct gggcaacgac gtactggcga gcatccagcg cctggacatt ggcctcggtt
     1261 tcgaaagcgg tatggccgtg gtgctgctgg cgatcattct tgaccgcatc acggaaagtt
     1321 tcggtacgcc gcaaaccgcc aaacgcagca gcctgttcgg ctggttcagc ggcaaacttc
     1381 aacgccaata agccttagct ttcgccatca cagggagtcg cagatgaaaa cgattaaaca
     1441 gctcgcaggt gtcggcctgc tctccgtggc catgctgcaa agcgcctggg cgcaggagcc
     1501 ggagagttgc aagatggtgc gcttcgccga aatcggctgg gccgacatcg ccgccaccac
     1561 tggcgtggcc atgaccctca gcgaaggcct gggctaccag acccgcaaaa tcatggcctc
     1621 cgtgcctatc gcctttaccg gcgtacagaa aggccagatc gacgtcttcc tcggttactg
     1681 ggcgccctcg atggacgctg tgatcgaacc cttcactaag gatggcggcg tcaaagtact
     1741 gcccaaagcc aacctcgaag gcgccaaata caccctcgcc gtcccgacct acgccgctga
     1801 agccgggctg aaaagctttg ccgacatcgc caagttcaag gaccagctgg gcggcaagat
     1861 cttcggcatc gagccgggca acgacggtaa cctgctgatt gagggcatga tcaagagcaa
     1921 ccagttcgac ctcggcgact tcaagatgat cgagtccagc gaagccggca tgctggtgca
     1981 gctgcaacgc gcggtgaaga agaaagaacc tgccgtcttc ctcggctggg ccccgcaccc
     2041 gatgaacacc cagttcgaca tcacctacct ggaaggcggc gatgacgtct tcggcccgga
     2101 cttcggcgcc gccaaggtct acaccgtagt accgccagac tacgaagcac gctgcgccaa
     2161 cgtcggaaaa ctgctgaaca acctgcagtt caccgtggaa atcgaaagcc agctgatgga
     2221 aaaggttctg gataaacaga accccaccac cgtcgccaaa gagtggatca aagccaaccc
     2281 ggcaatcctc gaccagtggc tggccggcgt aaccacctat gacggccagg acggcgttgc
     2341 agcggtgaag aaacacctcg gcatgtaagc cacaccgcgc cgccccagtt atctgccggc
     2401 ggcgcggcta gttaaagcac cttgatcagc acccgacccg cgaggccaca cggccatcgc
     2461 ctggtttatt gcagcccgga tttaacacgc agcacctgag caacaccgat taacccctca
     2521 ccccactggg gaaacgactg catagatgca gaaagtagg
//
LOCUS       DASRUM010000225         6847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_950744, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000225 DASRUM010000000
VERSION     DASRUM010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6847
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950744"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2741
                     /locus_tag="VFF22_15175"
     CDS             <1..2741
                     /locus_tag="VFF22_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HZX18191.1"
                     /translation="LAADLKRFEASGSRMALFAALRRHLNVKPLNPAGYAWLEVVANT
                     AAETKHAVADIINVMLEELVHHRYELPAFSTLDRMAFRAREKSNNRYFSAITSQLTAH
                     TKALIDGLLKTATGESVSPWQMLKREPKRPTNKETRTYIQHIRRLQYLVELLPKPDVP
                     VPKLKQFRHLARALDAAEMAELKPQKRYALAVIFIRAQHAQSLDDAADLFIRLMQNLE
                     NNARQKLLSFQQERVQKTDMLVGQLKDILGAYQLEGTDTQRVEAISTTLVAEVDELLN
                     ECEQHLAYAGRNHLPFLLQPYKMVRAQLLNCIGIASPKASSEDQVVERLIEALYKLRD
                     NRTDIVLLDMLGLSEDKDLRWMSAQWKKLVLVRPSGKGRAEAVHRRYFELAVMHAVKD
                     DLKSGDLFIKYGERYDDYREQLVDDETFEKEVGGYGQVTGIETDPGTFVTMLKSAMAQ
                     RARDIDATFPENAHAEIVDGRLILRKPPRSEIVEAAARIDALITERMQAASIVDVMID
                     TERWLDLHKLFRPLAGTDSRLADLRMRVITTLFCYGCNLGPVQTAKSIKGLSRRQISW
                     LNLKYVSEDLLDKAIVKVINAYNKFELPGYWGTGKHASADGTKWNLYEQNLLSEHHIR
                     YGGYGGIGYYHVSDKFIALFSHFISCGTYEGIHILDGLMTNESDIRPDTIHGDTQAQS
                     YPVFALAHLLGIQLMPRIRGIQDLKFHRPEPGNAYRNINALFSDVIDWRLIELHLPAM
                     LRVAVSIKTGKITPSAILRRLGTYSRKNKLYFAFVELGKVIRTMFLLSYIGDVGLRKV
                     IHAETNKSEQFNVFAGWSFFGGEGIIAENIRHEQRKVIKYNHLVANMIILHNVVGMTR
                     VLQELRDEGTEITLEILGGLAPFRTAHINRFGDYTLDFKRKIGPLNFDATIIPMES"
     gene            2819..5788
                     /locus_tag="VFF22_15180"
     CDS             2819..5788
                     /locus_tag="VFF22_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021138877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HZX18192.1"
                     /translation="MPRRSILSATERESLLALPDAKDDLIRHYTFNDTDLSVIRQRRG
                     PANRLGFAIQLCYLRFPGIFLGADEPPFPPLLRMVAAQLKVPVESWNEYGQREQTRRE
                     HLVELQTVFGFKPFTMSHYRQAVHTLTELALQTDKGIVLASTLVENLRRQSIILPAMN
                     AIERASSEAITRANRRIYAALTDSLLSPHRQRLDELLKRKEGSKMTWLAWLRQSPVKP
                     NSRHMLEHIERLKAWQALDLPAGIERQVHQNRLLKIAREGGQMTPADLTKFEVQRRYA
                     TLVALAIEGMATVTDEIIDLHDRIIGKLFNAAKNKHQQQFQASGKAINDKVRMYGRIG
                     QALLEAKQSGGDPFAAIETVMPWERFAASVTEAQKLAQPADFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKGVLDAIDVLRGMNNDNARKVPADAPTAFIKPRWAKLVLTDD
                     GIDRRYYELCTLSELKNALRSGDVWVQGSRQFKDFDEYLVPVEKFVTLKLASELPLAV
                     ATDCDQYLKDRLTLLEAQLATVNRMAVANDLPDAIITESGLKITPLDAAVPETAQALI
                     DQSAMLLPHVKITELLMEVDEWTGFTRHFTHLKSGDTAKDKTLLLTTILADGINLGLT
                     KMAESCPGTTYAKLSWLQAWHIRDETYSTALAELVNAQFRQPFAANWGDGTTSSSDGQ
                     NFRTGSKAQSTGHINPKYGSSPGRTFYTHISDQYAPFSAKVVNVGVRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFGLMHLLGFRFAPRIRDLGDTKLFIPKGDTAYDALKP
                     MISSDRLNIKQIRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGR
                     IERTLFILDWLQSVELRRRVNAGLNKGEARNALARAVFFYRLGEIRDRSFEQQRYRAS
                     GLNLVTAAIVLWNTVYLERATSALRSHSQTVDDTLLQYLSPLGWEHINLTGDYLWRSS
                     AKVGAGKFRPLRPLPPA"
     gene            complement(5795..5950)
                     /locus_tag="VFF22_15185"
     CDS             complement(5795..5950)
                     /locus_tag="VFF22_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18193.1"
                     /translation="MDMKAHRHPAVHSPDHRTDPAALKDPVCGMAVTEQSLHQAEHEG
                     VVSENGK"
     gene            complement(6013..>6847)
                     /locus_tag="VFF22_15190"
     CDS             complement(6013..>6847)
                     /locus_tag="VFF22_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016779387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HZX18194.1"
                     /translation="LAWVTNNGTPKSAAAGMAHAGMSTAEHGALSQAGEVWSIDTETD
                     EVVARVVVGKHPAHVVVSADGRWAYVTNGGDNTLSVVDTKSLKVVDTIPTGEFPHGIR
                     LSPDGKQAYVANLKGGTVSVIDTESRKEISQIPVGKGPAQVGFTSDSRLAFVSLSQEA
                     SVAVIDPVSRKVIRKVRVGNVPIQLYATPDNRFLFVANQGSRKSPGNTVSVIDLSTFS
                     TQRIQTGIGAHGVVVGNDGRHAYVTNLYANTVSVIDVRSRKSVATVAVGVGPNGISVS
                     P"
BASE COUNT         1396 a         2121 c         2040 g         1290 t
ORIGIN      
        1 ccctggcggc ggacctcaag cgcttcgagg cctcgggctc gcgcatggcg cttttcgcgg
       61 ctttgcgccg gcacttgaat gtcaaaccct tgaacccggc gggctacgcc tggctggagg
      121 tcgtggccaa cacggcggcc gagaccaaac acgcggtggc cgacatcatc aacgtgatgc
      181 tggaggaact ggtccaccac cgctacgaat tgccggcctt ctcgacgctg gaccggatgg
      241 cgtttcgtgc ccgggaaaag agcaacaatc ggtatttctc cgcgatcacc agtcagttga
      301 cggctcatac caaggccctg atcgacggct tgcttaaaac cgccacgggt gagtcggttt
      361 ctccctggca aatgctcaag cgcgaaccca agcggccgac caacaaggaa acgcgcacct
      421 acattcagca catccggcgc ctgcaatacc tggtggagct gcttcccaag cccgacgtgc
      481 cggtgcccaa gctcaagcag ttccggcacc tggcgcgcgc cctcgatgcg gccgagatgg
      541 ccgaactcaa gccgcaaaag cgctacgccc tggcggtgat cttcatccgg gcgcagcatg
      601 cccagtctct ggacgatgct gccgatctgt ttattcgcct gatgcagaac ctggaaaaca
      661 atgccaggca gaaattgctc tccttccagc aggagcgggt ccagaaaacc gacatgctgg
      721 tcggccagct caaagacatc ctgggcgcct accaattgga ggggacggac acccagcgcg
      781 ttgaagccat cagcaccacc ctggtggccg aggtggacga gctgctgaac gaatgcgagc
      841 agcacctggc ctacgccggc cgcaaccatc tgcctttcct gctgcagccc tacaagatgg
      901 ttcgggccca gttgctcaac tgcattggca tcgcctcgcc caaagccagc agcgaggacc
      961 aggtggtgga gcggctgatc gaggcgcttt acaagctgcg cgacaaccgc acggacatcg
     1021 tcctgctgga catgctgggc ctgagtgaag acaaggacct ccgctggatg tccgcccagt
     1081 ggaagaagtt ggtgctggtc cggccatcag gcaagggccg ggcagaggcc gtccaccgcc
     1141 ggtattttga actggcagtc atgcatgccg tcaaggacga cctgaagtcg ggcgatctct
     1201 tcatcaagta cggcgagcgc tatgacgact accgcgagca gctggtcgat gacgaaacct
     1261 ttgaaaagga ggtgggcggc tatggccagg tcaccggcat cgagaccgat cccggcacct
     1321 ttgtgacgat gctcaaatcc gccatggctc agcgggctcg ggacatcgac gccactttcc
     1381 cggaaaacgc ccatgctgag atcgtcgatg gccgcctgat tctgcgcaaa ccgccgcgct
     1441 ccgaaatcgt cgaggccgcc gcgcggatcg acgccctgat caccgagcgg atgcaagcgg
     1501 ccagcatcgt ggatgtcatg atcgacaccg aacgctggct cgacctgcac aagctgttcc
     1561 ggccattggc cgggacggac agccgccttg cggacctgcg catgcgcgtc atcacgacgc
     1621 tgttttgcta cggctgcaac ctggggccgg ttcagaccgc caaatccatc aaaggactga
     1681 gccgccgcca gatttcctgg ctgaacttga aatacgtcag cgaggatctt ctggacaagg
     1741 ccattgtcaa ggtgatcaat gcgtacaaca agtttgaact gccaggctac tggggcaccg
     1801 gcaagcacgc gtcggccgac ggcacgaagt ggaacctcta cgagcagaac ttgctgtccg
     1861 agcaccatat ccgctatggc gggtacggcg gcatcggcta ctaccatgtg tccgacaagt
     1921 tcatcgcgct gttcagccat ttcatttcct gcggcaccta tgagggcatc cacatcctgg
     1981 atgggctgat gaccaacgaa tcggacatcc ggccggacac gattcacggg gacacgcagg
     2041 cccagagtta cccggtcttc gcgctggccc acttgctggg catccagctc atgccgcgca
     2101 tccggggtat tcaggacctc aaattccatc gccccgagcc gggcaatgct taccgtaaca
     2161 tcaatgcgct gttcagcgat gtgatcgact ggcggctgat tgaattgcat ttgccggcga
     2221 tgctccgggt ggcggtttcc atcaagaccg gcaagatcac gccgtcggcc atcctgcgcc
     2281 ggctcggcac ctacagccgc aagaacaaac tgtatttcgc gtttgtggaa ctcggcaagg
     2341 tcatccggac catgttcctg ctgagctaca tcggcgatgt cgggctgcgc aaggtgatcc
     2401 atgccgaaac caacaagagc gagcaattca atgtctttgc cggctggtcc ttcttcgggg
     2461 gagaggggat cattgcggag aacatccggc acgagcaacg caaggtgatc aagtacaacc
     2521 acctggtggc caacatgatc attttgcaca acgtggtggg catgacgcgg gttctgcagg
     2581 agcttcgaga tgagggcacc gagatcacgc tggagatcct ggggggcctg gcgccgtttc
     2641 gcacggctca catcaacagg tttggggact acacgctgga cttcaaaagg aagatcggtc
     2701 ctttgaattt cgatgccacc ataattccaa tggaatcata ggcttaaggt cgtttctgcg
     2761 gttttttaca tgaatccctg ccgaccctct tgtaccagta cctgcgggaa gactgacaat
     2821 gccgcgccgc tcaatcctgt ccgccaccga gcgcgaaagc ctgctggcac tgcctgatgc
     2881 caaagacgat ctgatccggc actacacgtt caacgacacc gacctgtcgg tcatccgtca
     2941 gcgtcgtggc cccgcaaatc ggttgggctt cgccatccag ctttgctatt tgcgattccc
     3001 tggcattttt ttgggtgccg atgaacctcc gttcccgccc ttgttgcgca tggtggctgc
     3061 gcaactcaag gtacctgtgg aaagctggaa cgagtacggc cagcgcgagc agacacggcg
     3121 ggagcacctg gtcgagctgc aaacggtgtt tgggttcaag cctttcacca tgagccacta
     3181 ccgacaagcg gtgcatacgc taacggagct ggccttgcag accgacaagg gcattgtgct
     3241 ggccagcacc cttgtcgaaa acctgcgacg gcagagcatc atcctgcccg ccatgaatgc
     3301 catcgaacgc gcgagctccg aggccatcac ccgtgccaac cggcgtattt acgcggcgtt
     3361 gaccgattct ttgttatcac cccaccgtca gcgcctggac gaacttctca agcgcaagga
     3421 aggcagcaaa atgacgtggc tggcatggct gcgccagtcg cccgtcaaac cgaactcacg
     3481 gcacatgctc gaacacatcg agcgcctcaa ggcgtggcag gcgcttgacc tgcccgcagg
     3541 catcgagcga caggttcacc agaaccgact gctcaagatc gcccgtgagg gtgggcagat
     3601 gacgcccgcc gacctaacca agttcgaagt gcaacgacgc tatgccactc tggtagcgct
     3661 ggccattgaa ggcatggcca ccgtcaccga tgaaatcatc gaccttcacg accgcatcat
     3721 cggcaagctg ttcaacgccg ccaaaaacaa gcatcagcag cagtttcagg cttccggcaa
     3781 ggcgatcaat gacaaggtgc ggatgtacgg gcgcatcggt caagccctgc tggaagcaaa
     3841 acagagcggc ggcgatccgt tcgccgccat cgaaaccgtc atgccgtggg aaaggtttgc
     3901 agccagcgtc accgaggcgc aaaagcttgc gcaacctgct gatttcgatt ttttgcaccg
     3961 catcggcgaa agctatgcca cgttgcgccg ttacgcacca gagtttctgg ccgtgctcaa
     4021 gctgcgggcg gcaccggccg ccaagggcgt gctcgatgcc atcgacgtgc tgcgcggaat
     4081 gaacaacgac aacgcacgca aggtgcccgc cgacgcgccg accgcattca tcaagccacg
     4141 ctgggccaaa ctggtgctca cagacgatgg catcgaccgt cgctactacg agctgtgcac
     4201 cctgtcggag ttgaagaacg cgctgcgctc gggtgatgtc tgggtgcagg ggtcacgcca
     4261 gttcaaggac ttcgacgaat acctggtacc ggtcgagaag ttcgtcactc tgaagctggc
     4321 cagcgagttg ccgctcgccg tggccaccga ctgcgaccaa tacctgaaag accggctgac
     4381 gctgctggaa gcgcagcttg ccaccgtcaa ccgcatggcg gtggccaacg acctgccgga
     4441 tgccatcatt accgagtccg gcctgaagat cacgccgctg gacgcggcgg tgccggagac
     4501 cgcgcaagcc ctgatcgacc agtcggcgat gttgctgccg cacgtcaaga taaccgaact
     4561 gctgatggaa gttgatgagt ggacaggctt cacccggcac ttcacgcatt tgaagagcgg
     4621 cgacacggcc aaggataaga ccttgctgct gacgacgatc ctggctgatg gcatcaatct
     4681 tggactcacc aagatggccg agtcctgccc tggcactacc tacgccaagc tgtcgtggct
     4741 gcaagcctgg cacatccggg atgaaacgta ttcgacggcg ctggccgagc tggtcaacgc
     4801 gcagttccgg caacctttcg ccgcgaactg gggtgatggc accacttcgt cgtcggacgg
     4861 ccagaacttc agaactggca gcaaagcaca aagcactggt catatcaacc cgaagtatgg
     4921 aagcagtccc ggacggactt tctacaccca tatctccgac cagtacgcgc ccttcagcgc
     4981 caaggtggtc aacgtaggcg ttcgtgattc aacttacgtg cttgatggcc tgctgtacca
     5041 cgagtcggac ttgcgcattg aggagcacta caccgacacg gcaggcttca ccgatcatgt
     5101 gtttggcttg atgcacctgc tgggcttccg cttcgcgccg cgcatccgtg acctgggcga
     5161 caccaagctg ttcatcccca aaggcgatac ggcctatgac gcgctcaagc cgatgattag
     5221 cagtgaccgg ctgaacatca agcagatacg cgctcattgg gatgaaattt tgcggctggc
     5281 cacctcaatc aagcagggca cggtgacggc ctcgctgatg ttgcgcaagc tcggcagcta
     5341 tccacgccag aacggtctgg ctgtcgcctt gcgcgagttg gggcgcatcg agcgcacgct
     5401 gttcattctg gattggctgc aaagcgtgga actgcgccgg cgcgttaatg ccgggctaaa
     5461 caagggcgag gcgcgcaacg cgctggcccg cgccgtgttc ttctaccgat tgggtgaaat
     5521 tcgcgaccgc agcttcgagc aacaacgcta tcgggccagc ggcctcaacc tagtgacggc
     5581 ggccatcgtg ttgtggaaca cggtctatct ggagcgtgcc accagtgctt tgcgtagcca
     5641 cagccagacc gtcgacgaca cgctgttgca atacctgtca ccgctggggt gggagcacat
     5701 caatctgacc ggcgattacc tctggcgcag cagcgccaag gtcggcgcgg gcaagttcag
     5761 gccgttgcga ccgctgccac cggcttagcg tgctttattt tccgttttct gagacgaccc
     5821 cttcgtgctc cgcctggtga agcgattgtt cggtgacggc catgccgcac acggggtcct
     5881 tgagcgccgc cggatcggtc cggtggtcag gggaatgcac ggccgggtgc cggtgcgctt
     5941 tcatatccat ggcgttctcc ttccaggccg ctgcacacca gggcgacacg atggcatgac
     6001 cccgtttgtt tttcaaggac tcacgctgat cccgttgggt ccgacgccca cggcgacggt
     6061 ggcaacggac ttgcgcgacc ggacatcaat gacagacacc gtgttcgcat agagattggt
     6121 cacgtaggca tggcgcccat cgtttcccac caccacgccg tgggcgccga tgcccgtttg
     6181 gatgcgctgc gtgctgaagg tggagagatc gattacgctc acagtattgc cggggctctt
     6241 gcggctgccc tgattggcca caaagaggaa gcggttgtcg ggtgtcgcat agagctggat
     6301 cggcacattg ccgacccgca ctttgcggat caccttgcgg ctgaccgggt cgatgacggc
     6361 gacgctggcc tcttgcgaca gggacacgaa ggccagccga ctgtcggacg tgaagcccac
     6421 ctgggcgggg cccttgccca cggggatctg gctgatttcc ttgcggcttt ccgtgtcgat
     6481 gaccgacacc gttccgccct tcaggttcgc gacatacgcc tgcttgccat ccgggctcag
     6541 ccggatgccg tgaggaaatt caccggtcgg aatggtgtcg acgaccttga gcgatttggt
     6601 atccaccacg ctgagggtgt tgtcgccacc gttggtgacg taagcccaac ggccgtcggc
     6661 agacaccacc acatgggccg ggtgtttgcc caccaccacg cgggcgacca cctcgtcggt
     6721 ttccgtgtct atggaccaga cttcgccagc ttgggacagg gcgccgtgtt cggcggtcga
     6781 catgcccgca tgggccatgc cggcagccgc ggatttaggg gtgccgttgt tggtgaccca
     6841 ggccagc
//
LOCUS       DASRUM010000226         3845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1118620, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000226 DASRUM010000000
VERSION     DASRUM010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118620"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="VFF22_15195"
     CDS             complement(<1..1206)
                     /locus_tag="VFF22_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZX18195.1"
                     /translation="MAELMAILLFISICAALMAGYPVAFTLGGVSLLFAGIGILTGTF
                     DGGYLSALPNRLFGIMNNQTMLAVPLFVFMGVMLEKSRVAEDLLESMSRLFGTLRGGL
                     AISVCVVGALLAASTGIVGATVVTMGLLALPTMLRRGYDPAIATGTLAATGTLGQIIP
                     PSIVLVLLGDVMSSAYQQAQLKMGIFSPKTVSVGDLFVGALIPGLLLVGMYILYIIGV
                     AIFQPKKLPALPQEELGPIEWGKLINALVPPLLLIGAVLGSILAGYATPTEAAALGAL
                     GAMLLAFYKGKLNFGQLRQVAYGTTEISAMVFLILIGASLFSLVFRGFGGEAMIEDIF
                     AQLPGGVLGAFFLVMVVIFLLGFILDFIEITFVVVPIVGPVLLAMGLDPVWLGVMIAL
                     NLQTSFLTPP"
     gene            complement(1199..1744)
                     /locus_tag="VFF22_15200"
     CDS             complement(1199..1744)
                     /locus_tag="VFF22_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HZX18196.1"
                     /translation="MSDKPPLLLGLAHLIDSFNARFGKACAWLTLFLVLGTAIVVVLR
                     YGFGIGATALQEAVLYGHALVFMGAAAWVLQRNGHVRVDIFYQTFSPKRKALVDSLGT
                     LLFLLPVCLFLGWSSWDYVSNSWSTLEGSSESGGLKFVYLQKSIILVLVISLVLQGIS
                     DLIKFANILTGRMPAAEVNHG"
     gene            1941..3041
                     /locus_tag="VFF22_15205"
     CDS             1941..3041
                     /locus_tag="VFF22_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZX18197.1"
                     /translation="MKRRQILAAAGVGLAATALAGCKQEAETAGKPQEGASSQTFNWK
                     MVTSWPKNFPGVGVGAERFAKLVDEMSNGRLKVKVYAAGELVPALEVFDAVSRGTAEM
                     GHGAPYYWKGKVPAAQFFCALPFGPNAQEMNAWLHKGGGMQLWEETYKPFGVLPMACG
                     ATGVQTAGWFNKEINGVADFQGLKMRTPGLGGEVLTKMGGTVVNMPAGEIFTALQTGA
                     IDATEWIGPYNDQALGLHKAAKYYYTPGWQEPNVTFELDINIKAWETLPADLQAIVRA
                     AARDVNGDMLDDYNAKNMEALEQLKGEGVEVRRLPDEVLAKLKEVAAEVVDASAAADP
                     VASKVWAQQKAYLKRLYEYAELNEKDIYNIRG"
     gene            complement(3104..3505)
                     /locus_tag="VFF22_15210"
     CDS             complement(3104..3505)
                     /locus_tag="VFF22_15210"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020291610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZX18198.1"
                     /translation="MHDFEQEDPIPQGDLALQITALPRETNGFGDIYGGWLVSQMDLA
                     GTAMDSKVAGGRVATVAIDRMAFLVPVAVGAQLSFYTQTVEVGRSSIQMLVEVWSDDP
                     LSSEWRKVTEAVFVFVAIDGSGRTRPVPARR"
BASE COUNT          849 a         1126 c         1207 g          663 t
ORIGIN      
        1 cggcggggtg agaaaggacg tctgcaggtt gagcgcgatc atcacaccta gccacactgg
       61 gtccaagccc atggccagca ataccgggcc gacaatcggc accaccacga aggtgatctc
      121 gataaagtcg aggataaagc cgagcaggaa gatcaccacc atcaccagga agaacgcgcc
      181 aagcacgccg ccgggcaact gggcaaagat gtcctcgatc atcgcttcgc cgccgaagcc
      241 gcggaacacc agggagaaca acgatgcgcc gatcaggatg aggaacacca tggcgctgat
      301 ttcggtggtg ccgtaggcca cttggcgcag ctggccgaag ttcagcttgc ccttgtagaa
      361 ggccagcagc atggcgccaa gtgcgccgag tgcagcggct tcggtcgggg tggcgtagcc
      421 ggcgaggatc gagccgagta ccgcgccgat caacagcagc ggcggcacca gggcgttgat
      481 cagcttgccc cactcgatcg gacccagctc ttcttgcggc agggccggca gcttcttcgg
      541 ctggaagatc gccacgccga tgatgtagag gatgtacatg cccaccagca gcaaaccggg
      601 gatcagcgca ccgacgaaca ggtcaccgac cgagacggtc ttcggcgaga agatgcccat
      661 cttcagctgt gcctgttggt aggcgctgga catcacgtcg cccagcagca ccagcacgat
      721 ggacggtgga atgatttgcc ccagggtgcc ggtggcggcc agagtgccgg tggcaatcgc
      781 cgggtcataa ccgcggcgca gcatggttgg cagggccagc aggcccatgg tcaccacggt
      841 ggcgccgacg ataccggtgg atgcggcgag cagcgcgccg accacgcaca cggaaatcgc
      901 caggccgccg cgcagggtgc cgaacagccg cgacatggac tccagcaagt cttcggcgac
      961 cctggatttc tccagcatca ctcccataaa cacgaacagc ggcaccgcca gcatggtctg
     1021 gttgttcatg atgccgaaca ggcggttggg cagggcgctc aggtagccac catcgaaggt
     1081 gccggtgagg atgccgatac cggcaaacag cagcgataca ccgcctagag tgaaggccac
     1141 cgggtagccg gccatcaggg ccgcgcagat gctgataaac agcagaatcg ccatcaactc
     1201 agccatggtt cacctccgcg gctggcatgc ggccggtgag gatattggcg aatttgatca
     1261 agtcggagat tccttgtagg accaggctga tcaccagcac caggatgatg ctcttctgca
     1321 ggtagacgaa tttcaggccg cccgattcgc tggagccttc cagggtcgac caggaattgc
     1381 tgacgtaatc ccagctggac cagccgagga acaggcacac cggcagcaga aacagcagag
     1441 tgccgaggct gtcgaccagg gctttacgtt tggggctgaa cgtctgatag aagatgtcga
     1501 cgcgcacgtg gccgttgcgt tgcaacaccc aggcggcggc acccatgaac actaatgcgt
     1561 ggccgtacag cacggcttcc tgcagggcgg tggcgccgat gccgaaaccg taacgcagga
     1621 ccaccacgat ggcggtgcca agcaccagaa acagggtcag ccaggcgcag gctttgccga
     1681 aacgggcgtt gaatgaatcg atcagatgag caaggccgag cagcagagga ggtttatcgg
     1741 acattatgag tccttatggt tatgggcttt gtggacaggc aggccggcga ttctaggcag
     1801 gcctgtacgc atgcggcaat ggcactacaa ctttagtcgt atccgctagg cttgagagtc
     1861 acttacccct atgttactca ggcgcggtgg gtccgcagtg atcctgtggt cagaccacta
     1921 caacaataaa ggagctttgc atgaaacgtc gtcaaattct cgcagcagca ggcgtaggcc
     1981 ttgcagccac ggcactggcc ggttgcaaac aagaagctga aaccgctggt aagccccaag
     2041 aaggcgcgag cagccaaacc ttcaactgga aaatggtcac ttcctggccg aagaacttcc
     2101 ccggcgtagg cgtcggtgcc gagcgcttcg ccaagctggt tgatgaaatg agcaatggcc
     2161 gcttgaaggt caaagtctat gccgctggcg agctggtgcc ggcgctggaa gtattcgatg
     2221 cagtaagccg cggcactgct gagatgggcc acggcgcgcc ttactactgg aagggcaaag
     2281 tgccggccgc gcagttcttc tgcgccctgc cgtttggccc caacgcccag gaaatgaatg
     2341 cctggctgca caaaggcggc ggcatgcaac tgtgggaaga aacctacaag ccgttcggcg
     2401 tactgccaat ggcctgtggc gcgactggcg tgcagaccgc aggttggttc aacaaggaaa
     2461 tcaacggcgt agccgatttc cagggcctga agatgcgtac cccaggcctc ggcggcgaag
     2521 tgctgaccaa gatgggtggc accgtggtca atatgccggc cggtgagatc ttcactgcgt
     2581 tgcagaccgg cgccatcgac gccaccgagt ggatcggccc atacaacgac caggctctgg
     2641 gcctgcacaa agccgccaag tactactaca ccccgggctg gcaagagccg aacgtaacct
     2701 tcgaactgga tatcaacatc aaggcttggg aaaccctgcc ggccgaccta caggccatcg
     2761 ttcgcgccgc cgctcgtgat gtgaacggcg acatgctcga cgattacaac gcgaagaaca
     2821 tggaggccct ggagcagctc aaaggcgaag gcgtggaagt gcgccgtttg ccggacgaag
     2881 tgctggccaa actcaaggaa gtggccgccg aagtggtcga tgccagcgcc gccgccgatc
     2941 cggtagcaag caaggtatgg gctcagcaga aggcttatct caagcgtctg tacgagtacg
     3001 ccgagctgaa cgagaaggat atctacaaca tccgcggctg atttctagcg cacagcaaaa
     3061 cgccggcttt tgagccggcg ttttcgtttc tgcagttttg ccgttaacgg cgcgccggta
     3121 ccggacgtgt gcggccgctg ccgtcgatgg cgacgaagac gaataccgcc tcggtgacct
     3181 tgcgccattc gctggacagc ggatcgtcgc tccacacttc aaccagcatc tggatcgaac
     3241 tgcggcccac ttccacggtt tgtgtataga aggacagttg cgcgccaacc gctaccggca
     3301 ccagaaaggc catgcgatcg atagccacag tggctacacg gccgccggct accttgctgt
     3361 ccatggccgt gccggccagg tccatctgcg ataccagcca gccgccgtag atatcgccaa
     3421 aaccgtttgt ttcacgcggc agtgcagtga tctgcaaagc caggtcgcct tgtgggatgg
     3481 ggtcttcctg ttcgaaatcg tgcatcggat aggctcgcgg cgcggttgtt tttattggcg
     3541 cggtgcgggg cttggcggtg gcggcgagtc aggctctgcg gctatcgcgg gtcggcccag
     3601 caggcccgcg ggagtgcggg cgcagagagt atagcgactc ggcagtcgca gggcgaccat
     3661 ggcacttggc cgcctgggag ttgcacctca gttcatcgac tacggcaaaa cattggccgc
     3721 gttcatctaa ctgtcacatt gcttacatac agtcgtcatg cggcctacag atacttggcc
     3781 ccgttcaatc caacactagt ctgctaggag taaggcatga aacttaagcg tttgatggcg
     3841 gccat
//
LOCUS       DASRUM010000227         8078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1632633, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000227 DASRUM010000000
VERSION     DASRUM010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8078
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632633"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFF22_15215"
     CDS             <1..460
                     /locus_tag="VFF22_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009046784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HZX18199.1"
                     /translation="PAAPRDYGRYRTWSWRQLPAGSAWASSEQVQEALSNALDQRGLR
                     PHRAGATSDLKVSAELRLEQRIRQVTERYGSHYGHSRYGDDYGMWGSAPLVRSYTEEV
                     MVVRIELFDGQDGQPIWRASAETLSSGSQSERAAALRNAVQQALAAYPPN"
     gene            485..1048
                     /locus_tag="VFF22_15220"
     CDS             485..1048
                     /locus_tag="VFF22_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003157753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HZX18200.1"
                     /translation="MRASLLLLPLLLILSACQTPQLERDFDPNRDFAAYRSWSWQEPA
                     VQYKPDDPRINSDLTGQRIRSAVSEQLDQRGLRPAAANTPGDLKVQVWMIVDNRQQQV
                     STQVGGGWGDPWHGGFWGGPSYVETRTLDYQVGTLQIDLLDGKDGKLVWRGSAEQVLR
                     SRQPSPTEREASIRSTVAEVLSQYPPR"
     gene            1080..1568
                     /locus_tag="VFF22_15225"
     CDS             1080..1568
                     /locus_tag="VFF22_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX18201.1"
                     /translation="MTHTAHLSHRPAAAADLAEIVGFPQNADELFFCYPKADWPLSIG
                     QLAAAMAERRDSTVVLLDGRVAGFANFYQWHYDDHCALGNLMVAPWARGHGVAHYLVA
                     VMEQLAREHYHAKRMQVSCFNANSAGLLLYPKLGYALSDIVERRDPAGQRVALIQFSK
                     TL"
     gene            complement(1577..2476)
                     /locus_tag="VFF22_15230"
     CDS             complement(1577..2476)
                     /locus_tag="VFF22_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX18202.1"
                     /translation="MDRLTATRVFVEVVERGSQTAAAEALEISRAMVSRYLGELEAWV
                     GARLLHRTTRKLSLTGAGEQLLGQCREMLAMADAMQSVSRTDEAAPRGTLRIACSQSL
                     AQAWLVHALDEFIRLYPQVSVDLLVGSQAVNLVEARIDLALRITNQLDPNLIARQLAV
                     CRSVVCATPAYLAKYGTPQRPEELAQHNCLSYAYFGRSIWEFTRAGEPHAVAVSGNLS
                     ANESMVLLEAVLADIGISLQPRYSVSGHLRSGALVQLLPDYQPQELGIHALYGTRRQM
                     PPALRVLLDFLIERLADAPDWDM"
     gene            2576..3448
                     /locus_tag="VFF22_15235"
     CDS             2576..3448
                     /locus_tag="VFF22_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019097256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZX18203.1"
                     /translation="MSILNHLRGLLASASLLALATTAAAQPLTLDVYNPGDKAIFPVT
                     STLISGERDAILVDAQFSTREAQELVERIQASGKNLTTIFISHGDPDFYFGLDTLTRA
                     FPKAKVLATPQTIAYIEKSREPKLAYWGPILKDSAPARTLVPQALQGDHLTLEGQTLQ
                     LIGHDPKHTSLWIPSIKAVVGGVLTYANIHPWIADAQTPQARQTWLKSLYELEALEPK
                     TLVPGHFMGQPKLNLDDLRFTRNYLNDLEAALPKAKNSQALIEAMKAKYPDLLDASSL
                     ELSAKVLKGEMQWP"
     gene            3605..4054
                     /locus_tag="VFF22_15240"
     CDS             3605..4054
                     /locus_tag="VFF22_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016703794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZX18204.1"
                     /translation="MPAPIWWLMALPFVAGACLPLQAGINGQLAKQVSSVMAAALVSF
                     AVGTLALLILVLAQREFPSLGALKGLTWWHWSGGLLGVMFIATAAFAGPKVGAVLFMA
                     LVIAGQLSMALTLDHFGWAGFREAPITAGKVGGLLLIVAGIWLIRRG"
     gene            complement(4051..5004)
                     /locus_tag="VFF22_15245"
     CDS             complement(4051..5004)
                     /locus_tag="VFF22_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HZX18205.1"
                     /translation="MMSAAKGVLGRLPDISPWKWALLLLALTLVYQIGVQHLDNRLYF
                     WIKTSWHETEWENRSLWLPGYRVQIDALPVAGVSDNLSGLTYDEGRDQLWAVVNNPEE
                     LLALSKDGELLARYPLSGFSDVEGITYLGDNLLLLAEERDQALVVVELPEKPGALFRE
                     DYRALTVGIDLGGNQGFEGVGYDRARDRLYVVKEHSPRKLYEIRGLKSSVQGDFNLQV
                     IDRQAWIEHKVFATDLSSVHYDEQTGHLALLSDESKVLIELDAEDGKLISFRSLLSGF
                     AGLKDSVPQGEGVTLDDQGNLYLVSEPNLFYRFERESRPGA"
     gene            5443..6930
                     /locus_tag="VFF22_15250"
     CDS             5443..6930
                     /locus_tag="VFF22_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HZX18206.1"
                     /translation="MLQSLKNTWFFNIRGDVLAGLVVALALIPEAIAFSIIAGVDPKV
                     GLYASFCIAVVIAFVGGRPGMISAATGAMALVMVTLVKNHGLEYLLAATLLTGVLQIG
                     AGYLKLGSLMRFVSRSVVTGFVNALAILIFMAQLPELTNVTWHVYAMCAAGLGIIYLF
                     PYVPKLGQVIPSPLVCILSMTAVAMYFGLDIRTVGDMGELPDTLPIFLWPDVPLTLET
                     LAIIFPYAAALAVVGLLESMMTATIIDDLTDTNSDKNRECKGQGVANIASGLLGGMAG
                     CAMIGQSVINVKSGGRGRLSCLVAGVVLLLMVVFLSDWVSQIPMAALVAVMIMVSIGT
                     FSWDSLRNLKKFPLSTNIVMLATVAVTVYTHNLAYGVFVGVLLAAMFFANKIGHFLYI
                     NSEADNEGNQRTYTVIGQVFFSSADKFVAAFDFKEAVSKVTIDLSRAHFWDITSVAAL
                     DKVVIKLRREGTEVEVLGLNEASATIVDRFGVHDKENATDLLSGH"
     gene            6943..7794
                     /locus_tag="VFF22_15255"
     CDS             6943..7794
                     /locus_tag="VFF22_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZX18207.1"
                     /translation="MTQVMACIDGSQSTRAVCDYAAWASQRLSAPLTLLHVLDDVQYP
                     ISPDLSGTIGLGSREHLLEELANLDQQRHKLAMEQGRLMLEAARERVQADGVEPAQLR
                     QRHGDLVDCLHELEADIRLLVIGRQGKTSDSLSQLVGSHLENVIRTLHRPILVSCGEF
                     KAPQSYMLAYDGSATARKSLDMIAASPLLKGLPCHLVMIGADTSDAWEQLKAAERVVQ
                     GSASEVHLAIRAGEVEPTLHAYQLEQGIDLLAMGAYGHSRIRQFLVGSTTSTLLRTSN
                     TALLILR"
     gene            7805..8062
                     /locus_tag="VFF22_15260"
     CDS             7805..8062
                     /locus_tag="VFF22_15260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZX18208.1"
                     /translation="MDGEEFGILMPNTPLEQARLLAEQFRLELEREGFNVPGALLPLT
                     ASLGVGCYDARRDSSAEGFFKRVDDALYQAKAAGCNRLELA"
BASE COUNT         1561 a         2671 c         2395 g         1451 t
ORIGIN      
        1 cccggccgcg ccccgcgact atggccgcta ccgcacctgg agctggcggc agttgccggc
       61 gggcagcgcc tgggccagct ccgagcaggt gcaggaagcc ctaagcaatg cccttgatca
      121 acgcggcctg cgcccgcatc gcgccggcgc aaccagcgat ctgaaggtca gcgccgagct
      181 gcgcctggaa caacgtatcc gccaggtcac cgaacgctac ggtagccatt acggccatag
      241 tcgctatggc gatgattacg gcatgtgggg tagcgcgcca ctggtgcgca gctataccga
      301 agaagtcatg gtggtgcgca tcgaactgtt cgacggccag gatggccagc cgatctggcg
      361 cgccagcgcc gaaacgctta gcagcggcag ccagagtgaa cgcgccgccg cgctgcgcaa
      421 tgcggtacaa caggccctgg cggcgtaccc gccaaactga ttgctgaacc cgaggagagc
      481 acccatgcgt gcatcactgc tgttattgcc cctgctgctg atcctcagcg cctgccagac
      541 gccgcagctg gaacgcgact tcgaccccaa tcgcgatttt gccgcctacc gcagctggag
      601 ctggcaggaa cccgccgtgc agtacaaacc cgatgacccg cgcatcaaca gcgacctcac
      661 cggccagcgc atccgcagcg ccgtcagcga gcagctcgac cagcgtggct tgcgcccggc
      721 agcggcgaac acgccaggcg atctcaaggt gcaggtctgg atgatcgtcg acaaccgcca
      781 gcaacaggtc agcacccagg ttggcggagg ctggggcgac ccatggcacg gcggcttctg
      841 gggtggccca agttatgtgg aaacccgcac cctggactat caggtcggca ccctgcagat
      901 tgacctgctc gacggcaaag acggcaagct ggtctggcgc ggcagtgccg aacaagtgct
      961 gcgcagccgc caacccagcc ccactgagcg cgaagcctcg attcgcagta ctgtcgccga
     1021 agtgctaagc caatacccac cgcgctgagc gcgcacgcct cactcaacaa ggatgttgca
     1081 tgacacacac tgcacacctc agccatcgcc ccgcagcggc ggctgacctg gccgaaatcg
     1141 tcggttttcc gcagaacgcc gacgagctgt tcttctgcta ccccaaggcc gactggccgc
     1201 taagtatcgg ccagttggcc gccgccatgg ccgaacgccg cgacagcacc gtagtgctgc
     1261 ttgatggccg ggtggccggc tttgccaact tctaccaatg gcactacgac gaccactgcg
     1321 cccttggcaa cctgatggtc gccccctggg cccgtggtca cggcgtggcg cattatctgg
     1381 tggcggtgat ggagcagctg gcgcgcgagc attaccacgc caaacgcatg caggtgtcgt
     1441 gctttaacgc caacagcgct ggcctgctgc tctacccgaa actcggctac gcgctaagcg
     1501 atatagtcga acgccgtgat ccagccgggc aacgagtggc gttgattcag ttcagcaaaa
     1561 cgctctgaac ccccagctac atatcccaat ccggcgcatc cgccagccgc tcgatcagaa
     1621 aatccagcaa cacccgcaac gccggtggca tctgccggcg cgtgccgtag agggcgtgga
     1681 tgcccagctc ctgcggctga tagtcgggca gcaactgcac cagcgccccg gagcgcaagt
     1741 gcccgctgac cgagtaacgc ggctgcaggc tgatgccaat atcggccagc acggcctcca
     1801 gcagcaccat ggattcattg gcgctgaggt tgccgctgac cgccacggca tgcggctcac
     1861 cggcgcgggt gaactcccag atgctgcggc caaaataggc gtaactcagg caattgtgct
     1921 gcgccagctc ttctggccgc tgcggcgtgc cgtacttggc cagatacgcc ggagtagcgc
     1981 acaccaccga gcggcacacc gccagctggc gagcgatcag gttgggatca agctgattgg
     2041 tgatgcgcag ggccaggtcg atacgcgcct ccaccagatt gaccgcctgg ctgcccacca
     2101 gcagatcgac gctgacctgc ggatacaggc gaataaactc atccagcgca tgcaccagcc
     2161 aggcctgggc cagcgactgg ctgcaggcga tgcgcaaggt gccgcgcggc gcagcctcat
     2221 cggtgcggct gacgctctgc atggcgtcgg ccatggccag catctcgcgg cactggccga
     2281 gcaactgctc gcccgcaccg gtcaggctca acttgcgcgt ggtgcggtgc agcaggcgcg
     2341 cgccgaccca ggcctccagc tcgccgagat agcgcgaaac catcgcccgc gaaatctcca
     2401 gcgcctcggc ggcagcggtc tggctgccgc gctccaccac ttcgacaaat acccgggttg
     2461 ctgttagccg gtccatgatt agcccattta acgcaataaa tatgcgcaga ttaaggggct
     2521 ttttgatcga tttaaagcaa ctaagatcgc ctcacttact caacaggaga tcagcatgtc
     2581 gatcctcaac cacttgcgcg gcctgctcgc cagcgccagc ctgcttgcct tggcgaccac
     2641 cgccgccgcc caaccgctga ccctggacgt ctacaaccca ggcgacaagg cgattttccc
     2701 ggtgacctcg acgctgatca gcggcgagcg cgatgccatt ctggtcgatg cgcagttctc
     2761 cacccgcgaa gcccaggagc tggtcgagcg tattcaagcc agcggcaaaa acctcacaac
     2821 catctttatc agccacggcg acccagactt ctacttcggc ctggacaccc tgacccgtgc
     2881 cttccccaag gccaaggtgc tggccacgcc gcaaaccatc gcctatatcg agaaaagccg
     2941 tgaaccgaag ctggcctact ggggcccgat cctcaaggac agcgcgccag cccgcaccct
     3001 ggtgccgcaa gccctgcaag gcgaccacct gacgctggaa ggccagaccc tgcaactgat
     3061 cggccatgat cccaagcaca ccagcctgtg gattccgagc atcaaggcag tggtcggcgg
     3121 cgtgctgacc tacgccaata ttcatccctg gattgccgac gcacaaacgc cgcaagcgcg
     3181 gcagacctgg ctgaagtctc tgtatgagct ggaagcgctg gagccgaaaa ccctggtacc
     3241 gggccacttt atgggccagc ccaagctgaa cctggatgac ctgcgcttta cccgcaacta
     3301 cctcaatgat ctggaagctg cactgcccaa agccaagaac agccaggcgc tgatcgaagc
     3361 gatgaaggcc aagtacccgg acctgctcga tgccagcagc ctggaactga gcgccaaggt
     3421 tctcaagggt gaaatgcagt ggccgtaagc acagccgctg actagtaaca cctcacacgc
     3481 aaaaggcctg ggaaaaaccc aggccttttt gcatgcgcta agcggcaatc tgacctggtt
     3541 gaggctgacg aggcggacag cttttaggca tactcatccg cccttgctgc cctggagatg
     3601 ccccatgccc gccccgatct ggtggttgat ggccctgcca ttcgttgccg gcgcctgcct
     3661 accgctgcaa gcaggaatca acggccaact ggccaaacag gtgtccagcg tgatggccgc
     3721 agcgctggtg tccttcgctg ttggcaccct ggcgctgctg attctggtgc tcgcccagcg
     3781 cgagttcccc agccttggcg ccttgaaagg cctgacctgg tggcactgga gcggcggtct
     3841 actgggcgtg atgtttatcg ccaccgctgc gtttgccggg cccaaggtcg gcgcagtgct
     3901 gtttatggca ctggtgatcg ccgggcaact gagcatggcc ctgaccctgg accacttcgg
     3961 ctgggccggc tttcgtgaag cgccgattac tgcgggaaaa gtcggcggcc tgctgctgat
     4021 cgtcgccggc atctggctaa tccggcgcgg ttaagcaccc ggccggcttt cgcgttcgaa
     4081 gcggtagaac aggttgggct cgctgaccag atagagattg ccctgatcat ccagggtcac
     4141 gccctcgccc tgcggcacgc tgtctttcaa cccagcgaaa ccgctgagca gcgaacggaa
     4201 gctgatcagc ttgccatctt cggcgtccag ttcgatcagc accttggact catcactgag
     4261 cagggccaga tgcccggtct gctcgtcgta gtgcaccgat gacaggtcgg tggcaaacac
     4321 cttgtgctcg atccacgcct ggcggtcgat cacctgcagg ttgaagtcgc cctggacgct
     4381 ggatttcagg ccgcgtatct catacagctt gcgcggtgaa tgctccttga ccacatacag
     4441 gcggtcccgc gcacggtcgt agcccacgcc ttcaaagcct tggttaccgc caagatcgat
     4501 acccacagtc agcgcccggt aatcctcacg aaacaaggcg ccgggttttt ccggcagttc
     4561 gaccaccacc agcgcctgat cgcgttcttc agccagtagc agtaggttat cgcccaggta
     4621 ggtgatgcct tcgacatcgc tgaagccgct gagcggataa cgcgccagca gctcgccatc
     4681 cttgctcagg gccagcagct cctcggggtt attgaccacc gcccagagct ggtcgcggcc
     4741 ttcgtcgtag gtcagcccgg acaggttgtc gctcaccccg gcgaccggca gcgcatcgat
     4801 ctgcacccgg tacccgggca accataggct gcggttttcc cactcggtct catgccagga
     4861 ggtcttgatc cagaaataca ggcggttatc cagatgctgc acgcctatct ggtaaaccag
     4921 ggtcagtgcc agcagtaaca gtgcccattt ccatgggctg atatcaggta gccgtcctag
     4981 cacccctttc gcagcggaca tcataaatag ccatactcgc gtgcttacgg gaaacagcag
     5041 tgtgctgcgt gaacatgaca gccctgcgtg cgaacaccga ttgatcggcc caagacaggg
     5101 ctgcggtttt aggagtgcac accaccgaag gagttcctcg cgccagccgc aaaccagccc
     5161 agtggctgcg gcggttggga ggcttatgtt aaggtgagcg cccttttcgc ccgaatggat
     5221 agagacaggt cgatatgaca ctggcccaac catcccgctg gctgcctacg ccgccatagc
     5281 gtttcgcccc ctccctgcat agcacggccg gtaccaccgt tgccgcagca ttgcccctgc
     5341 gcgccgcccg aaggctgcgt tgctatcgtt gtacccagtt tttattagcc actccgccct
     5401 gcccttgcgt gcatctgcgg aacccagcct ttggaattga ccatgctgca aagcctgaaa
     5461 aacacctggt tttttaatat ccgcggcgat gtgctcgccg gtctggtggt ggcgctggcg
     5521 ctgatcccgg aagccatcgc cttctcgatc atcgccggcg tcgaccccaa ggtcggcctg
     5581 tatgcctcct tctgtatcgc cgtggtgatc gcctttgtcg gtggccgccc aggcatgatc
     5641 agcgccgcca ccggggccat ggctctggtc atggtcaccc tggtgaaaaa ccacggcctg
     5701 gaatacctgc tggcagcgac cctgctgact ggcgtgctgc agatcggagc cggttaccta
     5761 aaactgggct cgctgatgcg ctttgtttcg cgctcggtgg tcactggttt cgtcaacgct
     5821 ctggcgattc tgatctttat ggcccaactg ccggagctga ccaacgtcac ctggcacgtc
     5881 tacgccatgt gcgctgcggg cctgggcatc atctacctgt tcccctacgt gccgaagctg
     5941 ggccaggtga tcccctcacc gctggtgtgc atcctgtcga tgaccgcagt ggccatgtat
     6001 ttcggcctgg atatccgcac cgtcggcgat atgggcgaac tgccggacac cctgccgatc
     6061 ttcctctggc cggatgtacc gctgaccctg gaaaccctgg ccatcatctt cccctacgct
     6121 gccgcactgg ccgtagttgg cctgctggaa tcgatgatga ccgccaccat catcgatgac
     6181 ctgaccgaca ccaacagcga caagaaccgc gaatgcaaag gccagggcgt ggccaatatc
     6241 gcctccggcc tgctgggcgg catggccggt tgcgcgatga tcggtcagtc ggtgatcaac
     6301 gtgaagtccg gcggccgtgg ccgtttgtca tgcctggtgg ccggcgtggt gctgttgctg
     6361 atggtggtgt ttctcagcga ctgggtcagc cagatcccca tggccgcgct ggtggcggtg
     6421 atgatcatgg tgtcgatcgg caccttcagc tgggactcgc tgcgtaacct gaagaaattc
     6481 ccgctctcga ccaatatcgt catgctcgcc accgtggcgg tcaccgtcta tacccacaac
     6541 ctggcgtacg gcgtgtttgt cggcgtgcta ctggcggcga tgttctttgc caacaagatc
     6601 ggccacttcc tctatatcaa ctcggaagcc gacaacgagg gcaaccagcg cacctacacg
     6661 gtaatcggcc aggtgttctt cagctccgcc gacaagttcg ttgccgcctt tgatttcaag
     6721 gaagccgtaa gcaaggtcac catcgacctg tcccgcgcgc acttctggga cataacctcc
     6781 gtcgccgccc tggataaagt cgtgatcaaa ctgcgccgcg aaggcaccga agtcgaagtg
     6841 ctgggcctga acgaggccag cgccaccatc gtcgaccgct tcggggtgca tgacaaagag
     6901 aacgccaccg acctgctgtc gggccactaa aaggaaatgc ccatgaccca agtaatggca
     6961 tgtatcgacg gctcacaatc cacccgcgcg gtctgtgatt acgccgcctg ggccagccag
     7021 cgcctgagcg cgccgctgac cctgctgcac gtgctggatg acgtgcagta cccaatcagc
     7081 cccgacctgt ccggcactat cggcctgggc agccgcgagc atctgctcga agagctggcc
     7141 aacctcgatc agcagcgcca caaactggct atggaacaag gccggctgat gctcgaagcc
     7201 gcccgcgagc gggtgcaggc cgatggcgtg gagccggcgc agctgcgcca gcgccatggc
     7261 gatctggtcg actgcctgca cgagctggaa gccgatattc gcctgctggt gatcggccgt
     7321 cagggcaaga ccagcgacag ccttagccag ctggtcggca gccatctaga aaacgttatc
     7381 cgcaccctgc accggccgat tctggtcagc tgcggcgagt tcaaggcgcc gcaaagctac
     7441 atgctcgcct atgacggcag cgccacggcg cgcaagagcc tggacatgat cgccgccagc
     7501 ccgctgctca aaggcctgcc ctgtcacctg gtgatgatcg gcgcggacac cagcgatgcc
     7561 tgggagcagc tcaaggccgc ggaacgggtg gtgcaaggtt cggccagcga agtgcacctg
     7621 gcgattcgcg ccggtgaagt ggaacccacg ctgcacgcct accagctcga acaaggcatt
     7681 gatctattgg ccatgggcgc ctacggccac tcgcggattc gccagttcct ggtgggcagc
     7741 accaccagca ctctgctgcg caccagcaac acggcgttgc tgattctgcg ctaggggtta
     7801 tgcaatggac ggcgaagaat tcggcatcct gatgcccaat accccgctgg agcaggcgcg
     7861 cctgttggcc gagcagtttc gtctggagct ggagcgcgaa ggcttcaacg tgccaggagc
     7921 gctcctgccg ctgaccgcga gcctcggcgt gggctgttac gacgcacgtc gcgacagcag
     7981 cgctgaaggg ttcttcaaac gcgtcgacga tgcgctgtat caggccaagg cagccgggtg
     8041 caatcgcctg gaactggcct gaaggcggcg gcacgaac
//
LOCUS       DASRUM010000228        15959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2284067, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000228 DASRUM010000000
VERSION     DASRUM010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 15959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15959
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2284067"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VFF22_15265"
     CDS             <1..1049
                     /locus_tag="VFF22_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019486194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZX18209.1"
                     /translation="PMLHPYDQDPPDGWLGTANQRTVPRGYGMQLSNSWFYPERAERI
                     AALAATGRHDTQSMIAMQYDQTSPFVAKLQNMFSAPGMAEGLKAAIDALPAAERSKAR
                     EAHSRLMKFDGKMAATSADAALYSAFLYQSARETFLDELGPDSSPAWKALVETANSSY
                     SAQADHLLGREDSPFWNDIRTPQREDKPAILARSLAGAVTLLESKLGSERSTWQWGKL
                     HTYSWTTETSQLAQYMSSSQRTSINAIKGYLDRGPYPAGGDHSTLNVAAYNWGENFDT
                     WLIPAMRIVVDFGREEPLIGLNSSGQSGNPASKHYADGIDAWLKGSYMSFPFQSQNLD
                     KVYGSKRLLLMPAN"
     gene            1358..2185
                     /gene="nadE"
                     /locus_tag="VFF22_15270"
     CDS             1358..2185
                     /gene="nadE"
                     /locus_tag="VFF22_15270"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HZX18210.1"
                     /translation="MSNRQAEIAAALDVVPPFADDAALVAQINQRKAFIKNCLRNSGL
                     KVLVLGISGGVDSLTAGRLAQLSVEELRSETGDNAYRFIAVRLPHGTQHDEQDAQDSL
                     KFIRADEEDTVNIADSVSGLGEQVAHLQKLSDARRDFVTGNIKARIRMVAQFAIANAN
                     NGLVIGTDHAAEAVMGFFTKFGDGACDLAPLSGLVKNQVRAIAKHLGAPDNLAFKVPT
                     ADLEELHPGKPDEEAHGVSYAEIDAFLHGQPVREEAYAIIVRTYDNTRHKRELPLAP"
     gene            complement(2527..3093)
                     /gene="dcd"
                     /locus_tag="VFF22_15275"
     CDS             complement(2527..3093)
                     /gene="dcd"
                     /locus_tag="VFF22_15275"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HZX18211.1"
                     /translation="MSIKSDKWIRRMAQEHGMIEPFVERQVRAEGAQPLISYGVSSYG
                     YDVRCADEFKVFTNINSATVDPKNFDEKSFVDVKSDVCIIPPNSFALARTVEFFRIPR
                     DVLTICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTTLPAKIYANEGVAQMLFL
                     QSDEACEVSYKDRGGKYQGQLGVTLPRA"
     gene            3418..3627
                     /locus_tag="VFF22_15280"
     CDS             3418..3627
                     /locus_tag="VFF22_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZX18212.1"
                     /translation="MSNRQNGTVKWFNDEKGYGFITPQSGDDLFVHFKAIQSDGFKSL
                     KEGQAVTFVATRGQKGMQAEEVQVV"
     gene            complement(3736..4827)
                     /gene="apbC"
                     /locus_tag="VFF22_15285"
     CDS             complement(3736..4827)
                     /gene="apbC"
                     /locus_tag="VFF22_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HZX18213.1"
                     /translation="MSVVTRAAVEAALSQYTDPHLNQDPVSAGCLREVDIQGGQVKVR
                     LQLGYAAGLFKSGWAQMLQMALENLDGVERAQVQVDCVIEAHKAQDQVPALANVKNVI
                     AVASGKGGVGKSTTAANLALALAREGAKVGILDADIYGPSQGIMFGIPEGTRPQVKDQ
                     KWFVPLKAHGVEVMSMAFLTDENTPVVWRGPMVSGALLQLITQTAWDDLDYLVVDMPP
                     GTGDIQLTLAQKVPVAGAVIVTTPQDLALLDAKKGVEMFRKVNIPVLGVVENMAVHIC
                     SNCGHAEHLFGEGGGEKLAAQFGVDLLASLPLSMAIRMQSDGGKPTTIADPESQIAMI
                     YQETARNVGARISQGGAQQAMPTIVISED"
     gene            4983..7025
                     /gene="metG"
                     /locus_tag="VFF22_15290"
     CDS             4983..7025
                     /gene="metG"
                     /locus_tag="VFF22_15290"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HZX18214.1"
                     /translation="MSQARKILVTSALPYANGSIHLGHMLEYIQTDMWVRFQKLRGNQ
                     AIYVCADDAHGSAIMLRAEKEGITPEQLIANVQAEHTADFADFGVNFDNYHSTHSDEN
                     RELSAAIYLKLRDAGHIATRSVTQYFDPEKGMFLADRFIKGTCPKCAAEDQYGDNCEK
                     CGATYEPTELKNPRSAISGAVPELRDSKHFFFKLPDFEAMLKSWTRGGALQDAVANKI
                     AEWLDSGLQEWDISRDAPYFGFEIPDEPGKYFYVWLDAPIGYMASFKNLCAKRPELDF
                     DAFWNKDSTAELYHFIGKDIVNFHALFWPAMLEGAGLRKPTAVAVHGYLTVNGQKMSK
                     SRGTFIKARTYLDHLNPEYLRYYYAAKLGRGVDDLDLNLEDFVQKVNSDLVGKVVNIA
                     SRCAGFIHKGNNGLLVAGNAAPELTEAFQAAAPSIAEAYEARDFARAMREIMALADRA
                     NAWIADKAPWSLAKQDGKQNEVQAICALGVNLFRQLVIFLKPVLPQLASDAEAFLNVA
                     PLTWDDHQTLLADHQLNPFSALLTRIEPAKIDAMIEASKEDLAASEAAPVAQGNGELT
                     KDPLAAEIAFDAFAAVDLRIALIEKCEFVEGADKLLRLTLDIGDAKRNVFSGIKSAYP
                     DPSKLEGRLTLYVANLAARKMKFGISEGMVLAAGPGGEEIYLLSPDSGAKPGQRVK"
     gene            7181..7765
                     /gene="rsxA"
                     /locus_tag="VFF22_15295"
     CDS             7181..7765
                     /gene="rsxA"
                     /locus_tag="VFF22_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HZX18215.1"
                     /translation="MTELALIMVSAILVNNFVLVQFLGLCPFMGVSKKIETAIGLSLA
                     TTFVLTLAAMCSYLVQQYVLKPLDIEYLRTISFILVIAVVVQFTEMVVNKTSPLLYRV
                     LGIFLPLITTNCIVLGVALLNANKAEFTFITATANGFAAGLGFSLVLVLFAAMRERIA
                     IADVPKSFQGAAIGMITAGLMSLAFMGFTGLIKL"
     gene            7762..8337
                     /gene="rsxB"
                     /locus_tag="VFF22_15300"
     CDS             7762..8337
                     /gene="rsxB"
                     /locus_tag="VFF22_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HZX18216.1"
                     /translation="MSLVLIAVLALLALCLVAGAILGFAAVRFKVEGNPIAEQINALL
                     PQTQCGQCGYPGCKPYAEAIAGGDKINKCPPGGEATIQALADLLDVEAEPLDAVEGEK
                     PQMVAYIREAECIGCTKCIQACPVDAIVGAAKQMHTVIVSECTGCDLCVEPCPVDCID
                     MLEVGSTVQSWKWEKPLLPGQLIASDREQAA"
     gene            8334..10835
                     /gene="rsxC"
                     /locus_tag="VFF22_15305"
     CDS             8334..10835
                     /gene="rsxC"
                     /locus_tag="VFF22_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014819506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HZX18217.1"
                     /translation="MSALHKAEALIWDIPGGIHPAERKELSNRTPIQPAPLPKRLTLP
                     LNQHIGAPAEPVVAVGERVLKGQLIAAATGFVSVPVHAPTSGTVSLIGPHPYPHVSGM
                     LAPAIVIDSDGLDEWIELSPQTDYRHLENSALVELIRQAGISGLGGAGFPTAVKLNAR
                     PTQKIHTLIINGTECEPYISADDLLMRERANELISGIDILVQLIQPDQVLIGIEDNKP
                     EAIASVRNALAERSYQLKVFPTKYPSGGEKQLIQILTGVEVPSGGLPADIGMLCQNVG
                     TCVAIHNAVIHGKPLISRITTLTGEALARPMNVEVLLGTPAGELLEFAGLNRSKLNRL
                     IMGGPMMGFTLPDFAVPVIKTTNCLLASTSAELPPPPPAMPCIRCGECAEACPASLLP
                     QQLHFFAIGQEHEQLKAHNLFDCIECGACAYVCPSSIPLVQYYRAAKGEIRDLEQKQL
                     KAEQSKQRFELRQERLRRAEEQKEAERQARAAKAAQAKAAQAQAAPAAASSDTAAAKP
                     AGLSDEQKKLKIEASMAQVALKKAEKQLATHASPELQAQVDELRSAAAIAQKALDSAM
                     AANPPAPAAAPVADDGALKKAKIDAAMLRAQVRKLEKLEAPSDEQQSELQQLRSQLEA
                     AEKLLSSLEAQAPAPAAKAAADDGALKKAKIDTAMLRAQVRKLEKLEVPSEEQQSELQ
                     QLRSQLEAAEKLLVSLEVQAPAVPVKAPDAEAPDPLKKAKIELAMKRAELKKAEKAGS
                     DEAALSSLREALAAAEQALHQAEDASGKPAPELVRTDKKPIDETTRALKTEVAFARAD
                     LRKLERDTDSPADALAAARQRLADAERQLQEHAGS"
     gene            10879..11910
                     /locus_tag="VFF22_15310"
     CDS             10879..11910
                     /locus_tag="VFF22_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HZX18218.1"
                     /translation="MALPRITSPHAKGSNRTQQVMLQVLLATVPGILALTWLFGAGTL
                     INLLWASVCALGVEAAILGLRKRPVLFFLKDYSVLVTAVLLALALPPYSPWWLTLVAT
                     GFAVLFGKQLYGGLGQNPFNPAMLGYVVVLISFPVEMTSWPAPHTVGLLEGLQQILGI
                     ANLPDGWAQATALDALKVNKSLTIDELWAQNPAFGYLGGAATEAVNLAFLAGGLYLLH
                     KRLFTWYAPVGMLAALAVMSLLFWNGSGSDSHGSPLFHLLTGATMLGAFFIVTDPVSG
                     ATSNLGRLIFGIGVGVLVYVIRAWGGYPDGVAFAVLLMNLAAPTIDYYTRPRTYGHRK
                     PTRGFKLGE"
     gene            11940..12554
                     /gene="rsxG"
                     /locus_tag="VFF22_15315"
     CDS             11940..12554
                     /gene="rsxG"
                     /locus_tag="VFF22_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HZX18219.1"
                     /translation="MLKNALVLGLFAIATVGLVAVLQQATAGRIANAEREAQVRALSE
                     ILPQDSYDNHLLDNSIQLHDPLLGSKSPQTAYIALKDGKPSAVILRATAPDGYSGAIH
                     LLIGIHADGRLAGVRVLGHRETPGLGDKIELAKSAWIRSFDGKSLNNPSEDGWAVKKD
                     RGEFDQFAGATITPRAVVKAVHKALQYFDANQEQLFAAQVKSNG"
     gene            12547..13260
                     /locus_tag="VFF22_15320"
     CDS             12547..13260
                     /locus_tag="VFF22_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HZX18220.1"
                     /translation="MASPSYREITLNGLWKNNPALVQLLGLCPLLGVSNSAVNALGLA
                     LASAVVLVCSNTAVSLVRGVVNTAVRLPAFVMIIAALTTCIELLMQAFTYELYQILGI
                     FIPLITTNCVILGRADGFAAKNDPARAAYDGLMMGLGFGVVLVMIGALRELFGTGAVF
                     ANMHLLFGPIAANWQLTLIPEYKGFLLAILPPGAFIVLGLLIACKNRVDEVLAERAKA
                     QQVDTPVQSRRVRVTGVIE"
     gene            13257..13895
                     /gene="nth"
                     /locus_tag="VFF22_15325"
     CDS             13257..13895
                     /gene="nth"
                     /locus_tag="VFF22_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HZX18221.1"
                     /translation="MNAAKRYEIFRRLHEDNPEPKTELAYSTPFELLVAVTLSAQATD
                     VSVNKATAKLFPVANTPEAIYALGVEGLSGYIKTIGLYNSKAKNVIEACRLLIEKHNS
                     QVPDNREDLEALPGVGRKTANVVLNTAFRQLAMAVDTHIFRVSNRTGIAPGKNVVEVE
                     KKLMKFVPKEFLLDSHHWLILHGRYVCQARKPRCGSCRIEDLCEYKQKTSDD"
     gene            14003..14182
                     /locus_tag="VFF22_15330"
     CDS             14003..14182
                     /locus_tag="VFF22_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019693351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18222.1"
                     /translation="MSTDKEELELDEDFVAEEADDAEAPVEVAKTNLTKRRIIDNFLE
                     ERRLHKQLAEYDFDL"
     gene            complement(14269..14661)
                     /gene="gloA"
                     /locus_tag="VFF22_15335"
     CDS             complement(14269..14661)
                     /gene="gloA"
                     /locus_tag="VFF22_15335"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HZX18223.1"
                     /translation="MRLLHTMLRVGDMDKSIAFYTEVLGMTLLRRKDYPEGQFTLAFV
                     GYGDEAHNSVIELTHNWGVESYELGTGYGHIALEVADVYKACEDIRTRGGKITREPGP
                     MKHGTSILAFVEDPDGYKIELLSPSRAD"
     gene            complement(14756..>15959)
                     /locus_tag="VFF22_15340"
     CDS             complement(14756..>15959)
                     /locus_tag="VFF22_15340"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HZX18224.1"
                     /translation="KVVLAYSGGLDTSVILKWLQDTYNCEVVTFTADLGQGEEVEPAR
                     AKAKAMGVKEIYIDDLREEFVRDFVYPMFRANTVYEGEYLLGTSIARPLIAKRLIEIA
                     NETGADAISHGATGKGNDQVRFELGAYALKPGVKVIAPWREWDLLSREKLMDYAEKHA
                     IPIERHGKKKSPYSMDANLLHISYEGGVLEDTWTEHEEDMWKWTVSPEAAPDAPTYIE
                     LTYRKGDIVAIDGKEMSPATVLAELNRIGGENGIGRLDIVENRFVGMKSRGCYETPGG
                     TIMLKGHRAIESITLDREVAHLKDELMPKYASLIYNGFWWSPERLMLQQMIDASQANV
                     NGVVRMKLYKGNVIITGRKSDDSLFDANIATFEEDGGAYNQADAGGFIKLNALRMRIA
                     AGKGRKML"
BASE COUNT         3343 a         5154 c         4555 g         2907 t
ORIGIN      
        1 acccgatgct gcatccctac gaccaggacc cgcccgatgg ctggctcggc actgctaacc
       61 agcgcaccgt gccgcgcggc tatggcatgc agctgtccaa ttcttggttc taccccgagc
      121 gcgccgaacg catcgccgcg ctggccgcaa cgggcaggca cgacacccag agcatgattg
      181 ccatgcagta cgaccaaacc tcgccctttg tcgccaagct gcagaacatg ttcagtgcac
      241 caggcatggc tgaaggcctg aaagcggcga ttgacgccct accggcagca gaacgcagca
      301 aagcccgcga agcgcacagc cggctgatga agttcgacgg caaaatggcc gccacttcgg
      361 cggacgctgc gctgtacagc gccttcctct accagagcgc gcgcgagacc ttccttgacg
      421 aactcggccc ggacagcagc ccggcctgga aagccctggt ggaaaccgcc aacagctctt
      481 actccgccca ggccgaccac ttgctgggcc gcgaagacag cccgttctgg aacgatatcc
      541 gcaccccgca acgcgaagac aaacccgcca tcctcgcgcg cagcctggcc ggagcagtca
      601 ccctgctgga aagcaaattg ggtagcgagc gcagtacctg gcagtggggc aagctgcaca
      661 cctacagctg gaccaccgaa accagccaac tggcgcagta catgagttcc agccaacgca
      721 ccagcatcaa cgccatcaag ggctacctgg accgcggccc ctacccggcg ggcggcgatc
      781 acagcacgct gaacgtggcg gcttacaact ggggcgaaaa cttcgatacc tggctgatac
      841 cggccatgcg catcgtcgtc gacttcggcc gcgaagagcc gctgatcggc ctcaacagct
      901 ctgggcaatc cggcaacccc gccagcaaac actacgccga cggcatcgac gcctggctca
      961 agggcagcta tatgagcttc ccgttccagt cgcagaatct cgacaaggtg tatggcagca
     1021 agcgcctgct gctgatgcca gcgaactagg tgtagccagg gcttgtcgac cgttcgtcgg
     1081 ccaccgctga accaatccaa cggcggccgc tctgagaaag tggacttact ccatagatca
     1141 tcatcgtttt agggcgcttc ttagcgccct ttcttttgtc tgcaattcag gcagccacgc
     1201 tgtagaccga cacctagcca agacatgcag ctctagcgtg cactttgcag ccagttcaac
     1261 gtaagctttg cgctttgata acgctgatgc cctagcgccg ggaattgctg ccgcgcaacc
     1321 gcataagcca tgtgttctgc tcctagagga ctttgaaatg agcaaccgcc aagctgaaat
     1381 tgccgccgcc ctcgacgtag taccaccctt cgccgacgac gctgcgctgg ttgcgcagat
     1441 caaccagcgc aaagccttta tcaagaactg cctgaggaac tccggtttga aagttctggt
     1501 gctgggtatc agcggcggtg tcgactctct cactgcaggg cgtctggccc aactgtcagt
     1561 ggaagaattg cgcagcgaga ccggcgacaa cgcctatcgc tttatcgccg tgcgcctacc
     1621 ccatggcacc cagcacgacg agcaggacgc tcaggattcg ctgaaattta ttcgcgccga
     1681 tgaagaagac acggtgaata tcgcggacag cgtgtcgggt cttggcgaac aggtcgccca
     1741 cttgcagaag ctcagcgatg cccgccgcga cttcgtcacc ggcaatatca aggcgcgcat
     1801 tcgcatggtc gcccagttcg ccatcgccaa cgccaacaat ggcttggtga tcggcaccga
     1861 ccacgccgcc gaagcggtga tgggtttttt caccaagttc ggtgacggcg cctgcgatct
     1921 ggcgccgctc tctggcctgg tgaaaaatca ggtacgggcc atcgccaagc acctcggcgc
     1981 tccagacaac ctggcgttca aggtgcctac ggctgatctg gaagagctgc accccggcaa
     2041 accagacgaa gaagcgcacg gcgtcagcta tgcggaaatc gacgcgtttc tacacggcca
     2101 gccagtgcgc gaagaagcct atgccatcat cgtgcgcact tacgacaaca cccggcacaa
     2161 gcgcgagctg ccattagccc cttaaagaag ttgccatggg tacaggcggc gcttgatctg
     2221 gtaataacgg ctcgcccggt tcagtaggct gatccggcag tactggctca tccggggtat
     2281 ctggctcagg ctgccccggg atgccgccag caaatctgac ggccccaagg ggcatggata
     2341 gggcgctgga taattgagac gcatgcatgt cgtttacctc cttcgatcag tagtgacact
     2401 gagcgttaga gcactgcgcc cgaacaagaa ttccaggaat atttaaccta ggcagcagcc
     2461 cgtctcggct aagcagttta ccgagcacac aacgggcaca tgaatctacg gatcaactga
     2521 actaagtcag gcccttggca gagtaacgcc cagctgaccc tgatacttac caccgcgatc
     2581 cttgtaagac acttcgcacg cctcatcgga ctgcaggaac agcatctgcg ccacaccttc
     2641 gttggcatag atcttcgccg gcaaggtggt ggtgttggag aattccaggg tcacgtggcc
     2701 ttcccactcc ggttccaacg gggtgacgtt aacgataatg ccgcagcgcg cgtaagtgct
     2761 cttgcccagg cagatggtca gcacgtcgcg cggaatgcgg aagaactcca cggtgcgcgc
     2821 cagggcgaag gagttcggcg gaatgatgca cacatcgctt ttcacatcga caaagctctt
     2881 ctcgtcgaag ttcttcgggt cgacggtcgc cgagttgata ttggtgaata ccttgaactc
     2941 atcggcgcag cgcacatcgt agccgtagct ggaaacgccg taagagatca gcggctgtgc
     3001 gccctcggca cgtacctggc gctcaacgaa aggctcgatc atgccgtgct cttgagccat
     3061 gcggcgaatc cacttatccg atttgatgct catggcggct gtcctgctat agcgaagggt
     3121 gaaaagtgca cgcatcttac cggggccagc gactgggttc aaaggctctt gagccagcgc
     3181 ggtgcgcaaa acggcaaaca acctgtctgt cgccaaataa cagaaagatg cacagagcat
     3241 tcgcgcttag cgttaaaagc gagtatggtt gccccactgt gctgcatgtg tcaccgcgaa
     3301 tcgctacttg atgcctagat ttcgatccaa acatcgtccg actcctagta actcgttgca
     3361 ctcagtcccg gcctgggctt ttccagggct gttaataaaa ttagtaggag acataacatg
     3421 tcgaatcgtc agaatggcac cgtcaagtgg tttaacgatg aaaaaggcta cggcttcatc
     3481 accccacagt ccggtgacga cctcttcgta cacttcaaag ctatccagag cgatggcttc
     3541 aagagcctga aagaaggcca ggcagtgact ttcgtcgcta cccgcggtca gaaaggcatg
     3601 caagctgagg aagtacaggt tgtataacct gcactaactg gcttgataaa aagccccgcc
     3661 tagtgcgggg cttttttatg ggcgcttata tatctgtacg tacgatgtgg cgcacttgca
     3721 tgcgccacta ccgaattagt cttcactaat cacgatggtt ggcattgcct gctgcgcacc
     3781 accctgggaa atccgcgcac cgacgttgcg tgcggtttcc tggtagatca tcgcgatctg
     3841 actttccgga tcggcgatgg tagttggctt accgccgtcg gactgcatgc gaatggccat
     3901 cgacagtggc aacgacgcca gcagatcgac gccaaactgc gccgccagct tctcgccgcc
     3961 gccttcaccg aacagatgct cggcatgccc gcagtttgag cagatatgca cggccatgtt
     4021 ttccaccacg ccgagcaccg ggatattcac cttgcggaac atctccacgc ctttcttggc
     4081 atccagcaga gccaggtcct gcggtgtagt gacgatcact gcgccggcca ccggcacttt
     4141 ctgcgccagg gtcagctgga tgtcgccggt gcctggcggc atatcgacca ccagataatc
     4201 caggtcatcc caggcggtct gggtgatcag ctgcagcaac gcgccagaca ccatcggccc
     4261 gcgccacacc accggggtgt tttcatcggt gagaaaggcc atggacatca cttccacacc
     4321 atgggccttg agcggcacaa accacttctg gtctttcacc tgcggccgcg tgccttccgg
     4381 aataccgaac atgatgccct ggctcggccc atagatatcc gcatcgagga tgccgacctt
     4441 cgcgccctcc cgcgccaacg ccagggccag gttggccgcc gtggtggact tacccacccc
     4501 gcccttaccg gaggccacag caatcacatt cttcacgttg gccagtgccg gcacctgatc
     4561 ctgcgccttg tgcgcttcaa tcacgcagtc aacctgcacc tgcgcacgct cgacgccgtc
     4621 gaggttttcc agagccatct gcagcatctg cgcccagccg cttttgaaca gcccagccgc
     4681 gtagcccaac tgcagacgca ccttgacctg cccaccctga atatccactt cacgcaggca
     4741 accggcgcta accgggtcct gattcaagtg cggatcggtg tattgggaca gggcggcttc
     4801 gaccgctgcg cgggtaacga cactcatgga aggctccgaa aggctgagta aagcaggcgg
     4861 acatattacc ccaggctacc ccataggcgc gcaccggcag gcattacccg cgcaaggatg
     4921 aaaaatatcc gccgccccta tatagttgcc ggcttccctg agttaccagt gcagccgatc
     4981 ccatgagcca agcccgcaag attctcgtta ccagcgccct gccctacgcc aacggttcga
     5041 tccaccttgg ccatatgctc gagtacatcc agaccgacat gtgggtgcgc ttccagaaac
     5101 tgcgcggcaa ccaggccatc tacgtctgtg cagacgatgc ccatggctcg gcgatcatgc
     5161 tgcgcgccga gaaggaaggc atcactccgg aacaactgat cgccaatgtg caggccgaac
     5221 acaccgccga cttcgccgac ttcggggtga acttcgacaa ctaccattcg acccactcgg
     5281 atgaaaaccg cgagctgtct gcggccatct acctaaagct gcgcgacgcc gggcatatcg
     5341 ccacccgctc ggtgacccag tacttcgacc ccgaaaaggg catgttcctc gccgaccgct
     5401 ttatcaaggg cacctgtccg aagtgcgcgg ccgaagatca gtacggtgac aactgcgaaa
     5461 aatgcggcgc cacctacgag ccgaccgaac tgaagaaccc gcgctcggcc atctccggcg
     5521 ccgtacccga gctgcgcgac tccaagcact tcttcttcaa gctgccagac ttcgaggcca
     5581 tgctgaagag ctggacccgt ggcggcgcgc tgcaggacgc tgtagccaac aagatcgccg
     5641 aatggctgga ttccggcctg caggaatggg acatcagccg cgatgcgccc tacttcggtt
     5701 ttgagatccc cgacgagccg ggcaaatact tctacgtctg gctggacgcg ccgatcggct
     5761 atatggccag tttcaagaac ctttgtgcca agcgcccgga gctggacttc gacgcgtttt
     5821 ggaacaagga ctcgaccgcc gagctatacc actttatcgg caaggacatc gtcaacttcc
     5881 acgccctgtt ctggccagcc atgctcgaag gcgcaggcct gcgcaagccg actgcagtgg
     5941 ccgtgcacgg ctacctgacc gtcaacgggc agaagatgtc caaatcccgt ggcaccttta
     6001 tcaaggcgcg cacctatctg gatcatctga accccgagta cctgcgctat tactacgcag
     6061 ccaagctcgg tcgtggcgtg gatgacctcg acctgaacct ggaagacttc gtacagaagg
     6121 tcaactcgga cctggtcggc aaggtggtca acatcgccag ccgctgcgcc ggctttatcc
     6181 acaaaggcaa caacggtctg ctggttgctg gcaatgccgc acccgagctg accgaggcct
     6241 tccaggctgc cgcaccgagc atcgccgaag cctacgaggc ccgcgatttc gcccgcgcca
     6301 tgcgcgagat catggccctg gccgaccgcg ccaacgcctg gatcgccgac aaagcaccct
     6361 ggtccctggc caaacaagac ggcaagcaga acgaagtgca ggccatctgt gccctgggcg
     6421 taaacctgtt ccgccagctg gtgatcttcc tcaagccggt actgccgcaa ctggccagcg
     6481 acgccgaggc cttcctcaat gtcgcaccgc tgacctggga cgatcaccag acgctgctgg
     6541 ccgaccatca actgaaccca ttcagcgccc tgctgacccg tatcgagcct gcgaaaatcg
     6601 acgccatgat cgaagcctcc aaagaagacc tggccgccag cgaagctgct ccggtcgccc
     6661 aaggcaatgg cgagctgacg aaagacccgc tagctgccga gattgccttc gacgcctttg
     6721 ccgcggtcga cctgcgtatc gccctgatcg agaagtgcga gttcgttgaa ggtgcagaca
     6781 agctgctgcg cctgaccctg gatatcggcg acgccaaacg caacgtgttc agcggcatca
     6841 agagcgccta cccggacccg agcaagctgg aaggccgcct gaccctgtac gtggccaacc
     6901 tggcggcgcg caagatgaag ttcggcatca gcgaaggcat ggttttggca gccggccctg
     6961 gcggcgagga aatctacctg ctcagcccgg acagcggtgc caagccgggc cagcgggtca
     7021 aataaacctg cgacccgata gcgcaggcga taaccaccga ctgcgctagc cttatggcag
     7081 caccatagga aggctgatcc ggcttgtatg gcaggatcgg cttaccgata atagccaccc
     7141 ctttcctgcg gcctttgccc gtccatcaat ggaacccgca atgaccgaac tcgccttgat
     7201 catggtcagc gccatcctgg tcaacaactt cgtgttggtc cagttccttg gcctgtgtcc
     7261 ctttatgggc gtctcgaaga agatcgagac ggctatcggc ctgtcgttgg ccaccacctt
     7321 cgtgctgacc ctggccgcca tgtgcagcta cctggtgcag cagtacgtgc ttaaaccgct
     7381 ggatatcgaa tacctgcgca ccatcagctt tattctggtg attgccgtag tggtgcagtt
     7441 caccgaaatg gtggtgaaca agactagtcc gctgctgtac cgcgtactgg ggatctttct
     7501 gccgctgatc accaccaact gcattgttct gggcgtggcc ctgctcaacg ccaacaaggc
     7561 cgagttcacc tttatcaccg ccaccgccaa cggctttgcc gccggcctcg gcttctccct
     7621 ggtgctggtg ctgttcgccg ccatgcgtga acgcatcgcg attgccgacg tgccgaaatc
     7681 cttccagggc gcggccatcg gcatgatcac cgccgggttg atgtccctgg cctttatggg
     7741 ctttaccggg ctgatcaagc tatgagcctg gtgctgatcg ccgttctcgc cctgctcgcc
     7801 ctgtgcctgg tggccggcgc catcctcggt ttcgcggctg tgcgtttcaa ggttgaaggc
     7861 aatccgattg ccgaacagat caatgccctg ttgccgcaaa cccagtgcgg ccagtgcggt
     7921 tacccgggct gcaagcccta cgccgaggcg atcgccggcg gcgacaagat caacaaatgc
     7981 ccgcccggcg gcgaagcgac catccaggcc ctggccgacc tgctcgacgt tgaagccgag
     8041 ccgctcgacg ccgtggaagg cgaaaaacca cagatggtcg cttacatccg cgaagccgaa
     8101 tgcatcggct gcaccaaatg catccaggcc tgcccggtgg atgccatcgt cggcgcggcc
     8161 aaacagatgc acacggtgat cgtcagcgaa tgcaccggtt gcgacttgtg cgtcgagccc
     8221 tgcccggtgg actgcatcga catgctggaa gtcggcagca cggtgcagag ctggaaatgg
     8281 gaaaaacccc tgctgcccgg ccaattgatt gccagtgacc gggagcaggc agcatgagcg
     8341 cattgcacaa agctgaagca ctgatctggg atatccccgg cggcattcac ccggccgagc
     8401 gcaaggagct gtccaaccgc acgccgatcc agccggcgcc gctgcccaag cgcctgacgc
     8461 tgccgcttaa ccagcatatc ggcgcgcccg cagaacctgt ggtcgccgtg ggtgagcgcg
     8521 tgctgaaagg ccagctgatc gccgctgcca cgggatttgt cagcgtaccc gtgcacgcac
     8581 cgacctccgg cacagtgagc ttaatcggcc cacaccccta cccgcatgta tccggcatgc
     8641 tcgccccggc catcgtgatc gacagtgatg ggctggacga atggatcgag ctgagtccac
     8701 aaactgacta tcgtcatctg gaaaacagcg ctctggttga gctgattcgc caggcgggca
     8761 tcagcggcct gggcggtgca ggctttccca cggcggtgaa actcaacgcg cggccgacgc
     8821 aaaaaatcca cacgctgatc atcaacggca ccgagtgcga gccctatatc agcgctgacg
     8881 acctgctgat gcgcgagcgt gccaacgagc tgatcagcgg catcgacatt ctggtgcagc
     8941 tgattcagcc agatcaggtg ctgatcggca tcgaggacaa caagcccgaa gccatcgcct
     9001 ccgttcgcaa tgcgctggcc gagcgcagct atcagctcaa ggtattcccc accaagtacc
     9061 cgtccggtgg cgaaaagcag ctgatccaga ttcttaccgg cgtggaagtc ccgtccggcg
     9121 gcctgccagc agatatcggc atgctgtgcc agaacgtcgg cacctgcgtg gccatccata
     9181 acgcagtgat ccacggcaaa ccgctgatct cacgcatcac caccctgacc ggtgaggccc
     9241 tggcccgacc gatgaacgtc gaggtactgc ttggcacgcc ggcgggtgaa ttgctggagt
     9301 ttgctggcct gaaccgcagc aagttgaacc gcctgatcat gggcggcccg atgatgggct
     9361 ttaccctgcc cgacttcgcc gtacccgtga tcaagaccac caactgccta ctggcctcga
     9421 ccagcgccga gctgccacca ccgccgccgg ctatgccctg catccgctgc ggcgaatgcg
     9481 ccgaagcctg cccggccagc ctgctgccgc agcaactgca cttcttcgcc atcggccagg
     9541 agcacgagca gctcaaggcg cataacctat tcgactgcat cgaatgcggc gcctgcgcct
     9601 atgtctgccc gtcgagcatc cctctggtgc agtactaccg cgccgccaag ggcgagattc
     9661 gcgacctgga acagaaacag ctcaaggccg agcagtcgaa acagcgcttc gaactgcgtc
     9721 aggaacgcct gcgccgcgct gaagagcaga aggaagccga acgccaggcc cgcgctgcca
     9781 aagcagccca agccaaggcc gcgcaagccc aagccgcacc agcggcggcc agcagtgata
     9841 cagcagcggc caaaccggcc ggcctgagcg atgagcagaa gaaactcaag atcgaagcca
     9901 gcatggccca agtcgccctg aaaaaggccg aaaagcagct ggcaacccac gccagccccg
     9961 agctacaggc ccaggtcgac gaactgcgca gcgcagcagc catcgcccag aaagcgctcg
    10021 acagcgccat ggccgccaac ccgccggccc cggctgctgc cccagttgcc gacgatggcg
    10081 cattgaaaaa ggccaaaatc gacgccgcca tgctccgcgc tcaggtgcgc aaactggaaa
    10141 aactcgaagc acccagcgac gaacagcaaa gcgaattgca acaactgcgc agccagctgg
    10201 aagccgcaga aaagttgctg tccagcctgg aagcccaggc accggcaccg gccgccaaag
    10261 cggctgccga cgatggcgca ttgaaaaaag ccaagatcga cacggccatg ctccgcgccc
    10321 aggtgcgcaa gctggaaaaa cttgaagtgc caagcgagga gcaacaaagc gaactgcagc
    10381 aactgcgcag ccagttggaa gctgcagaga agcttctagt cagcctggaa gtccaagcac
    10441 cggccgtccc ggtaaaagcg cctgatgcag aagcccccga tccgctgaaa aaagccaaga
    10501 tcgaactggc gatgaaacgc gccgaactga aaaaggccga gaaagccggc agcgatgaag
    10561 ccgcgcttag cagcctacgc gaggcattgg ccgccgccga gcaggcgctg caccaggccg
    10621 aggatgcatc tggcaagccc gcgccagagc tggtacgcac cgacaagaag ccaatcgacg
    10681 aaaccactcg cgccttgaaa accgaagtgg cctttgcccg cgcggatctg cgcaaactcg
    10741 aacgcgacac cgacagccca gccgacgccc tggcagccgc acgccagcgc ctggcggatg
    10801 ctgaacgcca attacaggaa cacgcaggca gctgataaca gccctgtgta gccaacccaa
    10861 gcaacaaccg gagcgccaat ggccctgccc cgtatcacat cgccccacgc caagggcagc
    10921 aatcgcaccc agcaagtgat gctgcaggtg ctgctggcta cggtgccggg cattctcgcc
    10981 ctgacctggc tgttcggcgc cggcaccctg atcaacctgc tgtgggccag cgtctgcgcc
    11041 ctgggtgttg aagcagccat cctggggctg cgcaaacgcc cggtgctgtt cttcctcaag
    11101 gactacagcg tgctggtcac cgccgtgctg ctggccctgg cgctgccgcc ctactcgccc
    11161 tggtggctga ccctggtggc caccggcttt gccgtgctgt tcggcaagca gctgtatggc
    11221 ggcctcgggc aaaacccctt caacccggcc atgctcggct atgtggtggt gctgatttcc
    11281 ttcccggtag agatgaccag ctggccagcc ccacatacgg tcggcctgct tgaagggctg
    11341 caacagattc tcggcatcgc caacctgccg gacggctggg cccaggccac tgcgctggat
    11401 gcgctgaagg tcaacaagag cctgaccatc gacgagctgt gggcacagaa cccggcattc
    11461 ggctacctcg gcggcgctgc cactgaggcg gtcaatctgg ccttcctcgc cggcggccta
    11521 tacctgctgc ataagcgcct gttcacctgg tatgcaccgg tgggaatgct cgccgccctg
    11581 gccgtgatga gcctgctgtt ctggaacggc tcgggctcgg actcccacgg ctcaccgctg
    11641 tttcacctgc tgactggcgc gaccatgctc ggcgccttct ttatcgtcac cgacccggtc
    11701 agcggcgcca ccagcaacct cggccggctg attttcggca tcggcgttgg cgtgctggtg
    11761 tatgtgattc gcgcctgggg cggctacccg gatggcgtgg ccttcgccgt gctgctgatg
    11821 aacctggcag caccgaccat cgactactac acgcggccgc gcacctacgg ccaccgcaag
    11881 ccgactcgcg gcttcaagtt gggagagtaa ccgtggccct gcctgaaatt agccgctcga
    11941 tgctaaagaa cgccctggtg ctcgggctgt ttgccatcgc caccgtgggc ctggtggccg
    12001 tgctgcaaca agccactgcc gggcgcatcg ccaacgccga gcgcgaagct caggtgcgcg
    12061 ccttgagcga aatcctgccc caggacagct atgacaacca cttgctggac aacagcatcc
    12121 agctgcacga cccgctgctc ggcagtaagt cgccgcagac cgcctatatc gccctgaaag
    12181 acggcaaacc cagcgccgtg atcctgcgcg ccaccgcgcc ggacggttac agcggtgcga
    12241 tccacttgct gatcggcatc cacgcagatg gccgcctggc cggcgtgcgc gtgcttggcc
    12301 accgggaaac ccccggtctg ggcgacaaga tcgagctggc caagagcgcc tggattcgca
    12361 gcttcgacgg caagtcactg aacaacccca gcgaagatgg ctgggcagtg aagaaagatc
    12421 gcggtgaatt cgaccagttt gccggcgcca ccatcacccc gcgtgcagtg gtcaaggcgg
    12481 tacacaaggc cctgcagtat ttcgatgcca atcaagagca gctgttcgca gcccaggtga
    12541 agtccaatgg ctagtcccag ctaccgcgag atcacgctta acggcctgtg gaagaacaac
    12601 cccgcgctgg tgcaattgct cggcctctgc ccgctgctcg gggtgagcaa ctcggcggtc
    12661 aacgccctcg gcctggcgtt ggctagcgcc gtggtgctgg tgtgctccaa caccgcggtg
    12721 tcgctggtgc gcggcgtggt caacacggcg gtgcgcctac cggcctttgt gatgatcatc
    12781 gccgccctga ccacctgcat cgaactgctg atgcaggcct tcacctacga gctgtatcag
    12841 attctcggca tctttatccc gctgatcacc accaactgcg tgatcctcgg ccgcgccgat
    12901 ggtttcgccg cgaagaacga cccggcccgc gccgcctatg acggcctgat gatgggcctg
    12961 ggctttggcg tggtgctggt gatgatcggc gccctgcgcg agctgttcgg caccggcgcg
    13021 gtgttcgcca atatgcacct gctgttcggc cccatcgccg ccaactggca gctgacgctg
    13081 atccccgagt acaagggctt cctgctggcc atcctgccgc caggcgcctt tatcgtgctg
    13141 ggcctgctga tcgcctgcaa aaaccgtgtg gacgaagtgc tggccgagcg agccaaggcg
    13201 cagcaggtcg atacaccagt gcaaagccgc cgcgtgcggg tgaccggagt gatcgagtga
    13261 acgccgccaa gcgctacgag atcttccgcc gcctgcatga agacaacccc gagccgaaga
    13321 ccgaactggc ctacagcacg ccgtttgagc ttttggtagc ggtcaccctg tccgcccagg
    13381 ccaccgacgt cagcgtcaac aaggccaccg ccaagctatt cccggtcgcc aataccccgg
    13441 aggcgatcta tgccctgggc gtcgaggggc tgagcggata tatcaagacc atcggcctgt
    13501 acaacagcaa ggcaaagaac gtcatcgaag cctgccgcct gctgatcgaa aagcacaaca
    13561 gccaagtgcc ggacaaccgc gaagacctcg aagccctgcc cggggtcggg cgcaaaaccg
    13621 ccaacgtggt gctcaacacc gcgttccgcc agctggccat ggcggtggac acgcatattt
    13681 tccgcgtcag caaccgcacc ggcatcgccc ccggcaagaa tgtcgtggaa gtggaaaaga
    13741 agctgatgaa gtttgtgccc aaggaatttc ttctcgattc acaccactgg ctgatcctgc
    13801 acggccgcta tgtgtgccag gcgcgcaagc cgcgctgcgg cagctgccgc atcgaagatc
    13861 tatgcgaata caaacagaaa acctccgacg attgagccgc tattcagccg atcaataagc
    13921 cgattgaaaa aatctttttt acccgcccag gttttgccgc tataaggtgc gcaaatggcc
    13981 ctagtagcct ggagcggatt tgatgagcac tgataaagaa gaacttgagc tcgacgaaga
    14041 ttttgttgcc gaagaagcgg atgacgccga agcaccggta gaggttgcca aaaccaacct
    14101 gaccaagcgc cgcatcatcg acaacttcct cgaagagcgc cgcctgcaca agcaattggc
    14161 cgaatacgac ttcgacctct aacgccgcca gcaacaaaaa gccccgttac actgcggggc
    14221 ttttttgttg ctcaacagca atgacgaata tcctgcagtg cactggcttc agtcggcacg
    14281 cgatggcgac aacagctcaa tcttgtagcc gtccggatct tcgacaaacg ccagaatact
    14341 ggtgccgtgc ttcatcggcc cgggctcgcg agtgatcttg ccaccgcggg tgcgaatgtc
    14401 ttcgcaggct ttgtacacat cagccacttc caaagcgata tggccgtaac cggtgcccag
    14461 ctcgtaactc tccacgcccc agttatgggt cagctcgatc acgctgttgt gcgcctcatc
    14521 gccgtaaccg acgaatgcca gggtgaactg cccttctgga taatccttgc ggcgtagcag
    14581 ggtcatgcct aggacttcgg tgtagaacgc gatggattta tccatatcgc cgacgcgcag
    14641 catggtatgc agcagtctca tcaggtatct cctcatcgtt gcccatcttg cgggcttata
    14701 aatgcaaacc ccggcttatg gccggggttt ggtgtggctc aggtgctcca gtctatcaga
    14761 gcatcttgcg ccctttgccc gccgcgatgc gcatgcgcag ggcgttgagc ttgataaagc
    14821 cgccggcatc cgcctggttg taagcgccgc catcttcttc gaaggtggcg atattggcgt
    14881 cgaacagcga gtcatcggac ttgcgcccgg tgatgatcac gttgcccttg tacagtttca
    14941 tgcgtaccac gccgttcacg ttggcctggg aggcgtcgat catctgttgc agcatcagac
    15001 gctcagggct ccaccagaaa ccgttataga tcaggctggc gtatttcggc atcagctcat
    15061 ctttcaggtg agcgacttcg cggtccaggg tgatcgattc aatggcgcgg tggcctttga
    15121 gcatgatggt gccaccggga gtctcgtagc agccacggga cttcatgccg acaaagcggt
    15181 tttcgacgat atccagacga ccgataccgt tctcaccgcc gatgcggttg agttcagcca
    15241 gcacggtggc cgggctcatt tccttgccgt cgatggcaac gatgtcgccc ttgcggtagg
    15301 tcagctcgat ataggtcggt gcatctggcg cagcctcggg agagacagtc catttccaca
    15361 tgtcttcttc gtgctcggtc caggtgtctt ccagcacgcc gccttcatag gaaatgtgca
    15421 gcaggttggc atccatggag tacggcgact tcttcttgcc gtgacgttcg atcggaatgg
    15481 cgtgcttctc ggcgtagtcc atcagcttct cacgcgacag caaatcccac tcgcgccatg
    15541 gggcaatcac ttttacgcct ggcttcagtg cgtaggcgcc cagttcgaag cgcacctggt
    15601 cgttaccttt gccggtggcg ccgtgggaaa tggcgtcggc gccggtttcg ttggcgattt
    15661 caatcaggcg cttggcgatc agcgggcggg caatcgaggt gcccagcagg tattcgcctt
    15721 cgtagacggt gttggcgcgg aacatcggat agacgaaatc acgcacgaat tcttcgcgca
    15781 ggtcgtcgat gtagatttcc ttgacgccca tggccttggc cttggcgcgt gccggctcga
    15841 cttcctcacc ctggccgagg tcagcggtga aggtcaccac ttcgcagtta taggtatctt
    15901 gcagccactt gaggatcacg gaagtgtcca ggccaccgga atatgccagg actaccttg
//
LOCUS       DASRUM010000229        23312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_953933, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000229 DASRUM010000000
VERSION     DASRUM010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 23312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23312
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953933"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VFF22_15345"
     CDS             <1..371
                     /locus_tag="VFF22_15345"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HZX18225.1"
                     /translation="PGNLGVQDGHLTDCPPSPNCVSSQARNSEHRIAPLPLQGDPANT
                     RSRLLSLLAQTPRVAVVTQDAHYLRAEFTSQVMRFVDDVEFLIGPEAVEVRSASRLGH
                     SDLGVNRERIEGLRQQLSQP"
     gene            complement(402..1340)
                     /locus_tag="VFF22_15350"
     CDS             complement(402..1340)
                     /locus_tag="VFF22_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheV"
                     /protein_id="HZX18226.1"
                     /translation="MAGILDSVNQRTQLVGENRLEILMFRLAGRQLFAINVFKVQEVL
                     QMPKLTLMPQRHRFVCGVVNLRGQTLPVIDLSQAIGMRPIVPDERSTIIVTEYNRSVQ
                     AFLVGGVERILNLNWESILPPPGGAGRQHYLTAITKVEDQIVEVIDVEKVLAEITPMS
                     TKVSPEKLADPLLEFAKGREVLLVDDSNVAMSQLKGTMAQLDIRCHSASDGLRALNLL
                     KSWADEGIDVHEKLLMVITDAEMPEMDGYRLTTEIRNDSRLKDLYVVLHTSLSGSFNE
                     AMVKKVGCDNFLSKFQPDKLVDMVRQRLLLDEPQES"
     gene            complement(1494..2390)
                     /gene="yegS"
                     /locus_tag="VFF22_15355"
     CDS             complement(1494..2390)
                     /gene="yegS"
                     /locus_tag="VFF22_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HZX18227.1"
                     /translation="MQERKALLILHGKQALNAEVRAAVQERRDEGWQLDVRVTWEAGD
                     AQRLVNEALQAGYPTLIAGGGDGTLRDVAEAMALAKTTASLALLPLGTANDFAHAAGI
                     ALDPQIALALLDTPAQAIDLGEADGQVFLNMATGGFGSNVTANTSEDLKRVLGVAAYF
                     LTGLTRFAEVQSSFGRFTGPDFQWEGEFLALGIGNGRQAGGGHVLCPQARVNDGLLDI
                     CIVPAAVDVVGTLGTLLSGGINGLQSVALSARLPWLEVEAPEGLDLNLDGEPMESRKL
                     RFAAMPAALRLHLPEGSPLLLE"
     gene            2598..3296
                     /locus_tag="VFF22_15360"
     CDS             2598..3296
                     /locus_tag="VFF22_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020301483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HZX18228.1"
                     /translation="MIEVRPFAELGGAHHGWLNARHHFSFAEYYDPERMSWGPLRVWN
                     DDEIAPHTGFPTHPHRDMEIITYVRQGAITHKDNLGNQGRTEAGDVQVMSAGTGIAHS
                     EYNLEDSTTKIFQIWIQPNQSGLPPSWGAKPFPKGERAGAFVTLASGFAEDQDALSIR
                     SNARMAAATLQTGQSTEYPIGTARRAYLVAASGSIEVNGVSAQARDGVAVSDVEVIRV
                     TAIEDSEIILVDVA"
     gene            complement(3471..5030)
                     /locus_tag="VFF22_15365"
     CDS             complement(3471..5030)
                     /locus_tag="VFF22_15365"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HZX18229.1"
                     /translation="MSEKNYLLAIDNGTQSIRALLFDLQGNLLAKGKVELEAYYSKQP
                     GWAEQDPEYYWASLGQACAQLWQQVDIDRSHIRGVSLTTQRGTLINVDQQGQPLRPAM
                     LWLDQRQAKVEGRITGPWGWLFKLAGLQATVDYFRAQAEVNWIAQNQPDIWANTHKVL
                     LLSGFLTHRLCGKFVDSLTSCVAYLPFDYKRLQWAKPGDWKWQAMPVRREQLPELCKP
                     GELLGHITATASRHTGIPEGLPLIAAGADKACEVLGAGGVEASTACLSYGTTATINTT
                     RSKYLETIPLIPPYPSAIPDHFNTEVMIYRGFWMVSWFKQEFGLREMQRAKELGVEPE
                     ALFDELVNSVPAGSMGLMLQPYWSPGVREPGLEAKGSIIGFGDVHTRAHIYRAILEGL
                     AYALRQGKEKIEKRSGTRITRLRVSGGGSQSDAAMQLTADIFGLPAERPHLYETSGLG
                     AAINCAVGLGLYPDYPTAIAAMTRVGQVFEPNPEAQSTYQQLYTQVYQRMYKQLKPLY
                     QTIRKITGYPA"
     gene            complement(5051..6634)
                     /locus_tag="VFF22_15370"
     CDS             complement(5051..6634)
                     /locus_tag="VFF22_15370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HZX18230.1"
                     /translation="MNNWNAAWREQALPELAARDWDLIVVGGGISGAGILREAARRGW
                     KCLLLEQRDFAWGTSSRSSKMVHGGLRYIAKGQFALTRDSVRERQRLLQEAPGLVDPL
                     SFIMAHYQGGFPGPKVFGSLLAVYDALAGKRNHLYYPLQQLRYLAPGLKEDGLLGGTR
                     FFDAVTDDARLVQRVLGEARAEGGEALNGMRVVELLRENGRVTGLLAEDSETGLRYGF
                     NTRAVVQATGAWADQLRRKTGLEHIRPLRGSHLLLPAWRLPVAHAFSFMHAADKRPVF
                     VFPWEGATVIGTTDLDHSVPLAQEARISREEVDYLLAACNQQFPSAGIRAADALSSWA
                     GVRPVVSDGVAGRKPSDEKREHALWVEPGCVTLAGGKLTTFRLLALEVLQACATMVGR
                     TVDDQGAATFAAVPSQPMPALSPAQQKRLIGRYGRALPQLLALVDELGAEQVAGTDTL
                     WVELAWAAERELVLHLDDLLLRRTRLGLLLARGGAAELPRVRALCQTRLGWSDARWQQ
                     EEQAYLQLWQRCYSLPAED"
     gene            complement(6637..8232)
                     /locus_tag="VFF22_15375"
     CDS             complement(6637..8232)
                     /locus_tag="VFF22_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZX18231.1"
                     /translation="MRRWNGWGDEATVVELPAHGEAFLAELVGPGQVLADASLEQVLA
                     QVPASRLQAHPLICLNAEDRVRHARGQSLPDWLAMRSGDFSVFPDGVAYPESAAQIRE
                     LLAWASQQDLIVIPYGGGTSVAGHINPQAGSKPVLTLSLERMGKLIEIDHDSLIATFG
                     PGATGPQVESQLHAQGYTLGHFPQSWELSTLGGWVASRSSGQQSLRYGRIEQLFAGGK
                     VETFAGTLEIPTFPASAAGPDLREILMGSEGRFGIISEVKVRITRLADQENFYAVFLP
                     NWQQALNAIRSLAQARVPLSMLRLSNAIETKTQLALAGHPQQIAWLEKYLALRGARDG
                     KCMLTFGVTGSRTQNAASLKQAKKLLKSFDGVFTGTLLGKKWAENRFRFPYLRHGLWA
                     AGYAVDTLETATDWSNVDSLLNKVETSLRNGLSEEGEQVHVFTHLSHVYNEGSSIYTT
                     YVFRPGANYAEAMARWQKLKHAASLTIAENRGTISHQHGVGRDHAPYLPVEKGELGMA
                     TLKALAGHFDPEQRLAPGVLIQD"
     gene            8353..9378
                     /locus_tag="VFF22_15380"
     CDS             8353..9378
                     /locus_tag="VFF22_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZX18232.1"
                     /translation="MTDNLGYTSVPSLLKYLRHAEQLGLDIDQALAAAGIKAEDLADN
                     GKRIPSETHERLLAHLLRVSGDPLFGLHCARFVQPGSWSVLGYITMNCATLGEAMSRI
                     VPYEKLVGDMGVSRVETGADHVKMIWTCRHQVEPIRRHMVENVLASWLLYARWIADMD
                     RSPNEVWFEHAQPEGAVLADYQTIFGCPIRFEQSCNALIVPLEYLGVPLRQADANLLR
                     TLEEHALTLMAGLDDNEPLPQRVKNALRLLLKDGLPRKERVAEKFNMTVRTLQRHLQL
                     AGTSYQQILDELRQELAEHYLLRSDLAIQDIASYLGFTESRSFHRSFKSWTGQTPGEF
                     RESQRNS"
     gene            complement(9453..10667)
                     /gene="glp"
                     /locus_tag="VFF22_15385"
     CDS             complement(9453..10667)
                     /gene="glp"
                     /locus_tag="VFF22_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HZX18233.1"
                     /translation="MSGCDKPGLMPVEAALARLLAQAEVSAIVECEWVKLSDASGRVL
                     AEPLIATLDLPPWDNSAMDGYALRLADWNGEPLVVSQRIQAGSAPAPLQPGTCARIFT
                     GAPLPAGADSVEMQENAVLGEDGRAAFSEPLKLGQNVRAQGQETRIGDSVLPAGTRLG
                     PIELGLAASLGCAELNVRRRPRVAVLSTGDELVEPGQPLGPGQIYNSNRRLLIAWLQR
                     LGCEVVDGGILLDDLELTRAALAALSDVDLILSTGGVSVGEADFLGMALREEGELALW
                     KLAIKPGKPLTCGQFRGVPVIGLPGNPASTLVTFGLLARPYLLRRLGVQRVEPLGFPL
                     PAGFTWSKPGNRREYLRARLENGRVVPYPNQSSGVLRSAAWAEGFAEVLEGTTLVEGD
                     SLRFIPLSEILG"
     gene            complement(10664..11203)
                     /gene="moaB"
                     /locus_tag="VFF22_15390"
     CDS             complement(10664..11203)
                     /gene="moaB"
                     /locus_tag="VFF22_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HZX18234.1"
                     /translation="MNHKAEAHFVPLNIAVLTVSDTRSLDTDTSGQLFVDRLQAAGHQ
                     LAARVLLKDDLYKIRAQVATWIAEDQVQVVLITGGTGFTGRDSTPEAVSCLLDKQVDG
                     FGELFRQISVPDIGTSTIQSRALAGLANGTLVCCLPGSTNACRTAWDGILGEQLDNRH
                     RPCNFVPHLKPVAACESRG"
     gene            11271..11852
                     /gene="mobA"
                     /locus_tag="VFF22_15395"
     CDS             11271..11852
                     /gene="mobA"
                     /locus_tag="VFF22_15395"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HZX18235.1"
                     /translation="MPAPATLPRCSVLLLAGGRGQRMGGADKGLLDWQGRPLIAWLHE
                     LVRPLTDDLLISCNRNAERYAPFADRLVSDDEADFQGPLAGIRAGLRVARQANVLILP
                     CDAPQLDRPLLEALLSLAGESAVVVRQGEFLEPLFSVIPRALHADLEQAWQAGERSPQ
                     RWLRSLNPLTVECPLGDPRLANFNTPELLTTRG"
     gene            11930..12151
                     /locus_tag="VFF22_15400"
     CDS             11930..12151
                     /locus_tag="VFF22_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HZX18236.1"
                     /translation="MSQRIIPAVLLTLGLVALTGCASPTVITLNDGREIQTIDEPNFD
                     EDSGFYEFEQLDGKRAKVNKDQVRTVKEL"
     gene            12293..12369
                     /locus_tag="VFF22_15405"
     tRNA            12293..12369
                     /locus_tag="VFF22_15405"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12327..12329,aa:Pro,seq:cgg)
     gene            complement(12424..13308)
                     /locus_tag="VFF22_15410"
     CDS             complement(12424..13308)
                     /locus_tag="VFF22_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HZX18237.1"
                     /translation="MSMVLGDALSLLLFRLHSGRLLGINLLKVQEIIPCPTLTKLPSR
                     HPHVRGVATLRGSALTVIDLGKAIGEQGVPGNNDGCLIVTELSRSRQGLHVQSVERIV
                     QCYTRDVRPPPAGSGSKAFITGVTQIDGKIVQILDIEKVLHELAPMVIEELDEQLLSE
                     RERALLKGRRVLVVDDSHVALHQTMITLRKLELDCQMVRSAADALELLQRQFDAGEPI
                     EVLVSDIEMPEMDGYDLVRTLRKKPDIGQIYVLLHTSLDSTMNTEKAKAVGANDVLTK
                     FSTVDLSKALVHAFESLQ"
     gene            complement(13355..13597)
                     /locus_tag="VFF22_15415"
     CDS             complement(13355..13597)
                     /locus_tag="VFF22_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HZX18238.1"
                     /translation="MLPECQLFGTLGCHLCEVAEALLMPFVEHGLLVELVDIAEHEHW
                     VEDYALRIPVLRRSDTGAELDWPFEAEQIVEFLRSA"
     gene            complement(13648..14802)
                     /locus_tag="VFF22_15420"
     CDS             complement(13648..14802)
                     /locus_tag="VFF22_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5610 domain-containing protein"
                     /protein_id="HZX18239.1"
                     /translation="MNPLAANLSSASRPTSVTAQPAVRNAADAQVTLANRLAERLGLE
                     PGALAGKANDYTPEKVAGRILGFIEQRLQSEQAAGADPSKLKGLLEQARSGVEKGFAE
                     ARKILDGMGVLQGKVASDIDDTYQKLQSGFSDLDKRFNPDAALVEGSTNIAAYSERFA
                     AQAETFEMQVTTRDGDRLRISIAQASANWSQSGVVASNNDNGSSVVASSQSGSLQIGA
                     WQVSVEGELDDEERAALEKLFGQVQDLSDKFYAGDLSGAFDRAMALEMDGEQLASMSL
                     RLTQTTVRQATDAYSAVAQDGGQAASAVNSSLIDYAQGLLEALRSANQVAEEGTAKTS
                     LLDLLKGGFSLDERFDSGRLDKAEQLNSRLLDGLQNLLNPALDSSKSTDA"
     gene            15062..16318
                     /locus_tag="VFF22_15425"
     CDS             15062..16318
                     /locus_tag="VFF22_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015802 - basic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family porin"
                     /protein_id="HZX18240.1"
                     /translation="MTQSPLARAVALATLGTCLTLPSLAQAEFIKDSKASLELRNFYM
                     NRDFRDPGAAQSKREEWAQGFLFKAESGFTEGTVGFGLDAYAGLGLKLSSDDDLAGTN
                     LLPNRPGDEGPNNYSEAAVAVKAKLSNTVLKAGGLMPKLPIVSSGDSRLLPQVFEGAM
                     ISSQEIAGLSLNAGQLRQVNERDSTDRRDIRASNYTSAGNPRGSDRYNFGGGTYSFNA
                     GNTTAGLWYGELQDVYDQKMYNLIHTQPIGDWKLGANLAYFDSSDNGRKLAGQIDNDL
                     TSLMLSAGIGAHTFRLGYQDSSGDNAFPFIAETDPAIANYVQILDFTRADEQSWQARY
                     DVNFATYGVPGLTAFVRYINGDGFDLADGSNGKEWERDLDITYTIQEGTFKNLAFRWR
                     NAMVRSDVLGDLDENRLIVSYTLPLF"
     gene            complement(16392..17600)
                     /locus_tag="VFF22_15430"
     CDS             complement(16392..17600)
                     /locus_tag="VFF22_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009050098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZX18241.1"
                     /translation="MENINSAVETLIHGSNTLFILLGAVMVLAMHAGFAFLEVGTVRQ
                     KNQVNALSKIISDFAVSALAYFFVGYWIAYGVTFLEPASVLTEGNGYALVKFFFLLTF
                     AAAIPAIISGGIAERAKFGPQLCATVLIVAFIYPFFEGVIWNGNYGVQVWLEAQFGAT
                     FHDFAGSVVVHAMGGWLAFGAVILLGRRDGRYRDGRLVAFAPSNIPFLALGSWILIIG
                     WFGFNVMSAQTLGSVSGLVAVNTLMAMVGGTVAALVVGRNDPGFLHNGPLAGLVAVCA
                     GSDLMHPVGALVTGAIAGALFVWAFTATQVKWKVDDVLGVWPLHGLCGVWGGIACGIF
                     GQEALGGLGGVSLASQFLGTALGVVVALIGGVLVYGVLKATVGIRLSQEEEYYGADLS
                     VHKIGAVSQD"
     gene            17978..18295
                     /locus_tag="VFF22_15435"
     CDS             17978..18295
                     /locus_tag="VFF22_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HZX18242.1"
                     /translation="MARKTPVSSSSDQLLDEFQALVRDTESLLQHSASLAGEQAEELR
                     EQIRSSLGRARSTLHNAEDALHARGKAALEATEGYVQTHPWQTLGIAAAIGLLLGMLI
                     SRR"
     gene            18308..18691
                     /locus_tag="VFF22_15440"
     CDS             18308..18691
                     /locus_tag="VFF22_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZX18243.1"
                     /translation="MDATPPTPPSRSSSPRRFASALLGLLQGHIALFGEELKEQQSHT
                     LNLLVLTSLSLLFGLLLIIGLSAALLIAFWETHRLLVISALCAFYALCMLGALITLLQ
                     RVRNAPAPFSASLEELARDREQLLP"
     gene            18688..19041
                     /locus_tag="VFF22_15445"
     CDS             18688..19041
                     /locus_tag="VFF22_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18244.1"
                     /translation="MTMPELPAKASRRELRKTLLRMRLEMHRQELRHETLVMLQPLRQ
                     AQHWRSHWREELGNSNAPLWVAGGALLLATIGIRRGQWRRWLRIALVAFPLLRRNPAR
                     PDTPTGTTKDTQAGD"
     gene            19116..20279
                     /locus_tag="VFF22_15450"
     CDS             19116..20279
                     /locus_tag="VFF22_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017679318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZX18245.1"
                     /translation="MFNGQPLAVFQPFIDTATGRIAGVEALARLRSADGRLHSAGPLF
                     SDPKTPPTALRRLDREVREQALTRFHEAPQDWFLSLNISPRWISRLRPEQALPSLKQL
                     QRSGIDPARIVFEITELGGASTRLPGVVERYRQAGARIAIDDFGAGYSQLDRVLALQP
                     DILKLDMRLFQQAARGGPSSEVVKALAQMAEKTGCWIIAEGVETEAELDFALECGARY
                     VQGYLFAKPEEAFFSSQAFVKRFAQLRDHYVQQKLAERARLMTLRQQLGELMNSFKAW
                     SEGGAQPDQLPQPHAYPWLLRIFQCDRYGTQLTANLEWREHSWRSDTRYVGHNWSWRP
                     YFYQLLAEGWEERRLILSSTYRDATTNQYCMTAGQFIDNGKRLLLIDIDAAGF"
     gene            20355..21686
                     /locus_tag="VFF22_15455"
     CDS             20355..21686
                     /locus_tag="VFF22_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020290147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HZX18246.1"
                     /translation="MDWQTLLNRERLGKPTHSVAELGRTPFHKDHDRIIFSGAFRRLG
                     RKTQVHPVSSNDHIHTRLTHSLEVSCVGRSLGMRVGEMIRDALPDWCEPSDLGMVVQS
                     ACLAHDIGNPPFGHSGEDAIRHWFQQAAGRGWLDSMSAAERGDFLSFEGNAQGFRVLT
                     QLEYHQFDGGTRLTYATLGTYLKYPWTARHAEALGYKKHKFGCYQSELPLLEQIASKL
                     GLPQLEEQRWARHPLVYLMEAADDICYGLIDLEDGLEMELLDYSEVEALLLDLVGDDL
                     PETYRQLGPDDSKRRKLAILRGKAIEHLTNAAAEAFVEQQDALLAGTLQGDLVEHMHG
                     TAKRCVQSAKDMARQKIFQDKRKTLHEIGAYTTLEILLNAFCGAALEQHGGRTPSFKN
                     RRILDLLGNNAPDPNWPLYRSFLRMIDFIAGMTDSYATEMAREMTGRSSPV"
     gene            21690..22760
                     /locus_tag="VFF22_15460"
     CDS             21690..22760
                     /locus_tag="VFF22_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HZX18247.1"
                     /translation="MNSFLRQTFSWHAGPPAWGPAMVAGFGCALPLLLGLFSAHPGFL
                     WASAGAFQAAQANPLHRLGMLRMLMLCGLGACSAGLGFWSAEHAYASLLIFAAFGFLL
                     AWLQRFGSEAGKLGIGLAICLCLGQGQQGLGSLNNPYAIAMLFILGGLWVMLLAFGLR
                     GMHGLRMWPYMPRFISIFKVLKRHAKRLPQQKWRLHALGCTLAFALSGLIVNLAELHR
                     GYWLTLTVVTTLQLNFQGSLVRAVQSILASLGAAALLILLGHSMQDPALMVATLLPLI
                     ALSRALQANHYGLFVLQTTVCFVLLAESLAHDWQLPQVRLFNSLIGVALALLVALLVY
                     GLERWLTHRHNLHKAKHATDPI"
     gene            22930..>23312
                     /locus_tag="VFF22_15465"
     CDS             22930..>23312
                     /locus_tag="VFF22_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18248.1"
                     /translation="MLFTLLLLGSGVVLGYFNYQQTSNIILSSSDQLFSQMRKEVQAD
                     LRNTYQPIRYMINLLARNEQINGRDRHERMIMLPIFSQALRDNPKLASIYVGYGDGSF
                     FMVRPLRSEKLRQIFFAPDNAEYQV"
BASE COUNT         4840 a         7527 c         6662 g         4283 t
ORIGIN      
        1 cacctggcaa cctcggcgta caggatggcc atctgactga ctgcccgcca agccccaact
       61 gcgtcagcag ccaggcccgc aacagcgagc accggattgc cccgctaccc ctgcagggcg
      121 acccggccaa cacccgcagt cgcctgctca gcctgctggc gcagaccccg cgcgttgccg
      181 tggttaccca ggacgcgcat tacctacggg ccgagttcac cagccaggtc atgcgtttcg
      241 tcgatgatgt ggagtttctg atcggcccgg aggccgtgga agtgcgctcg gcgtcacgcc
      301 tcggtcactc ggacctgggc gtcaaccgcg agcgtattga aggtttgcgc cagcagctca
      361 gccagccctg agcgcttgcc agtgcggcga gttaagcaga ctcaggactc ttgcggctcg
      421 tccagcagca ggcgctggcg caccatatcg accaatttgt cgggctggaa cttggatagg
      481 aagttgtcgc aaccgacctt cttcaccatg gcttcgttga agctgccgga cagcgaggtg
      541 tgcagcacca catacaagtc cttgaggcgt gagtcattgc ggatttcggt ggtcaggcgg
      601 taaccgtcca tctccggcat ttccgcatcg gtaatcacca tcagcagctt ctcgtgcaca
      661 tcgatccctt cgtcggccca gctcttcagc aggttcagcg cgcgcaggcc gtcactggcg
      721 gagtggcaac gaatatccag ctgcgccatg gtgcccttga gctgactcat ggccacgttg
      781 gaatcgtcca ccagcaacac ttcacggccc ttggcaaact ccagcagcgg gtcggcgagt
      841 ttttccgggg aaaccttggt gctcattgga gtgatttccg ccagcacctt ttccacgtca
      901 atcacttcga cgatctggtc ttcaaccttg gtgatggcgg tcaggtaatg ctgacgcccg
      961 gcgccaccgg gtggcggcag gatggattcc cagtttaggt tgagaatacg ctcgacccca
     1021 ccgaccagga aagcctgcac cgaacggttg tattcggtca cgatgatggt gctgcgctca
     1081 tccggcacga tgggacgcat gccgatggcc tgggacagat caatcaccgg cagggtttgc
     1141 ccgcgcaggt tgaccacgcc gcaaacaaac cggtgccgct gcggcatcag ggtcagcttg
     1201 ggcatctgca ggacttcctg caccttgaac acgttgatcg caaacagctg ccgacccgcc
     1261 aggcggaaca tgagaatttc caggcggttc tcacccacca gctgggtgcg ttggttcact
     1321 gagtcgagaa tgccggccat cttgcgctcc tgttcactta tcgatctagc ctagctcaaa
     1381 tagccatgcc ctggtgtatc ggccatctgc cgcgcatctg aaggcagatg gcgcggcgcc
     1441 atcatgcgac aagcgcagca ccgaggccag ccagacgaca gtaagcgctt gatttactcc
     1501 agcagcaacg gcgagccttc cggtaggtgc agccgcaggg cagccggcat tgcggcaaag
     1561 cgcagcttgc gactctccat cggctcgcca tccagattga ggtccagccc ttctggcgcc
     1621 tcaacttcca gccagggcag ccgcgcgctt agggccacgc tctgcaaacc attaataccg
     1681 ccggacaaca atgtgcctaa ggtgccgacc acatcgaccg ccgccggaac aatacagata
     1741 tccagcagac catcattgac tcgcgcctgg gggcacagca cgtgtccacc gcccgcctgg
     1801 cgaccattac caatgcctag agcgagaaac tcgccttccc actgaaaatc cggaccggta
     1861 aagcggccga acgaagactg cacctcagca aaacgagtca gcccagtcag aaagtaagcc
     1921 gcgacgccca atacacgctt gaggtcttcc gaagtattgg cggtgacatt ggagccaaaa
     1981 ccgccagtgg ccatattgag aaacacctgc ccgtcggctt cacccagatc aattgcctgc
     2041 gctggcgtat ccagcagcgc caaagcaatc tgcggatcaa gggcgattcc cgccgcgtgg
     2101 gcaaagtcat tggccgtacc cagcggcaac agcgccaaac tggcagtggt cttcgccagg
     2161 gccatggctt cggccacatc acgcaaggtg ccatcgccac caccggcgat cagggtcggg
     2221 tacccggcct gcagcgcctc attgaccaga cgctgcgcat cgcccgcttc ccaagtcacc
     2281 cgcacatcca gttgccagcc ctcgtcacga cgttcctgca ccgcagcacg cacctcagca
     2341 ttgagggctt gcttgccatg cagaatcagc agcgctttac gttcttgcat tcccggcatc
     2401 cttttcatgc tttagatcag actgagcaac atggcttgag attgcattag cttgcaacct
     2461 gccggctaaa acatcaataa aacagataat taagctcaat aattacgcat ttttaatcgt
     2521 attgatttat tggatcatct gtccatcgcc caccaacact gcagccaaaa gccggtgttc
     2581 ttaatgagga tacgcagatg atcgaagtac gccccttcgc cgaactcggc ggtgcccatc
     2641 atggctggct gaatgcccgt caccactttt cctttgccga atactacgac ccggagcgca
     2701 tgagctgggg tccgctgcgg gtctggaacg atgatgagat cgccccacat acgggctttc
     2761 ctacccaccc acaccgcgac atggaaatca tcacctatgt acgccaaggg gccatcaccc
     2821 acaaggacaa cctgggtaac cagggacgca ccgaagcggg tgatgttcag gtgatgagcg
     2881 ccggcaccgg gattgcccac agcgaataca acctggaaga cagcaccacc aagatctttc
     2941 aaatctggat tcagccaaac cagtcaggtc tgccgccatc ctggggcgcc aagccgttcc
     3001 ctaaaggtga gcgcgccggt gcctttgtca ccctggccag tggcttcgcc gaagaccagg
     3061 acgcgctgtc gatccgcagc aatgcgcgga tggccgccgc caccctgcaa actgggcaaa
     3121 gcaccgaata cccaattggc acggcgcgca gggcttatct agtggccgcc agcggcagca
     3181 ttgaggtcaa cggcgtcagc gctcaggccc gcgatggtgt ggctgttagc gatgtcgaag
     3241 tgattcgggt gacggctatc gaagatagcg aaatcattct ggtggatgtg gcctgacctg
     3301 cagccatggc atagcgacgc aacaggacct atgcgccgct atgcctcttt aggctagata
     3361 cccggtgatc ttacgaatac agcccgtagg aagggttagc cgaaggcgat acccatcaat
     3421 gtgccgatgg gtatcgctac gctcaaccca tcctacaaaa ctcgggcctt ttaagccgga
     3481 taaccggtga ttttgcggat ggtctggtac agcggcttaa gctgcttgta catgcgctga
     3541 taaacctggg tgtagagctg ctgataggtg ctctgcgcct caggattagg ttcaaacacc
     3601 tggcctaccc gcgtcatggc ggcgatcgcc gtggggtaat ccgggtatag gcccagccct
     3661 accgcgcagt tgattgccgc acccaggccg ctggtctcat agaggtgcgg gcgctcggca
     3721 ggcaggccga aaatatcagc ggtcagctgc atcgccgcat cgctttgcga gccgccaccg
     3781 gaaacacgca ggcgggtgat acgcgtgccc gaacgcttct cgatcttctc cttgccctgg
     3841 cgcagggcat aagccaagcc ttcaagaatc gcccggtaga tatgcgcgcg ggtgtgcacg
     3901 tcgccaaaac cgatgattga gcccttggcc tccaaccccg gttcgcggac gcccggcgac
     3961 cagtagggtt gcagcatcag gcccatggag ccagccggca cgctgttgac cagctcatcg
     4021 aacaacgcct caggctcaac gcccagctcc ttggcccgct gcatttcacg cagaccaaac
     4081 tcctgcttaa accagctgac catccagaag ccgcgataaa tcatcacctc agtattgaag
     4141 tgatcgggga tcgccgaggg gtatggcgga atcagcggga tggtttccag gtacttgctg
     4201 cgggtggtgt tgatggtcgc cgtagtgcca taggacagac aggccgtgct cgcctcgacc
     4261 ccgccagcac cgagcacctc acaggccttg tcggcgccgg cggcaatcaa cggcaggcct
     4321 tcgggaatcc cggtatggcg gctggccgtc gccgtgatat ggccgagtag ctcaccgggc
     4381 ttgcacagct ccggcaactg ctcgcggcgc accggcatgg cctgccattt ccagtccccg
     4441 ggcttggccc actgcaaacg cttgtagtca aacggcaggt aggccacaca gctggtcagc
     4501 gaatcgacaa acttgccgca cagccggtga gtaagaaagc cggaaagcag tagcaccttg
     4561 tgggtattgg cccaaatgtc cggctggttc tgcgcgatcc agttgacctc ggcctgggcg
     4621 cggaagtaat ccaccgtggc ctgcaaaccc gcgagtttga acagccagcc ccaaggcccg
     4681 gtaatccgcc cttcgacctt ggcttggcgc tgatcgagcc agagcatggc cgggcgcagc
     4741 ggctgacctt gctgatcgac attgatcagg gtgccgcgct gggtagtcag cgacacgccg
     4801 cggatgtggc tgcggtcgat atccacctgc tgccacaact gcgcacaggc ctgacccaga
     4861 ctggcccagt aatactcggg gtcctgctcg gcccaaccgg gctgcttgga gtaataggcc
     4921 tcaagctcca ccttgccctt ggcgagcaga ttgccctgaa gatcgaacag cagcgcccgg
     4981 atgctctggg tgccgttgtc gatggccagc aggtaatttt tctcgctcaa gacgccgtcc
     5041 ttcctattga tcaatcttct gcaggcaggc tgtaacagcg ctgccagagt tgcagatacg
     5101 cctgctcttc ctgctgccag cgcgcatcac tccagcccag gcgggtctga cacagcgcac
     5161 gaactctcgg cagctctgca gcccctccgc gtgccagcag caaacctaag cgagtacgac
     5221 gcagcagcaa gtcatccaga tgcagcacca gctcccgctc ggcggcccag gccagctcga
     5281 cccagagcgt atccgtacct gccacctgct cggcgcctag ctcatcaacc aacgccagca
     5341 attgcggcag cgctcggcca taacggccga tcaggcgctt ctgctgggcc gggcttagcg
     5401 cgggcatcgg ctggctgggc accgcggcaa aggtcgccgc cccctgatca tccaccgtac
     5461 gcccgaccat ggttgcgcag gcctgcagca cttccagcgc cagcaggcga aaagtggtca
     5521 gcttgccgcc ggccagggtg acgcagccgg gctcgaccca cagcgcatgc tcacgcttct
     5581 catccgaagg cttgcgcccc gcaacgccgt cgctgaccac cggacgcacg ccggcccaac
     5641 tcgacagcgc atccgcagcg cgaatcccgg cggaaggaaa ttgctgattg caggcagcca
     5701 gcaggtaatc cacctcttca cgactgattc gggcttcctg cgccaagggc accgagtgat
     5761 ccagatcggt ggtgccgatt accgtcgcac cttcccaggg gaagacaaac accgggcgtt
     5821 tgtcagccgc gtgcataaag ctgaaggcat gggccaccgg caagcgccag gcgggcagca
     5881 acagatggct accacgcagc gggcggatat gctccagacc agttttccgg cgtaactgat
     5941 cagcccaggc accagtggcc tgaaccacgg cgcgggtatt aaagccgtag cgcaggccgg
     6001 tttcactatc ctcggcgagc aggccggtca cccgcccgtt ctcgcgcagc agctcgacca
     6061 cgcgcatgcc gtttagcgct tcgccgccct cggcacgggc ctcacccagc acccgctgca
     6121 ccagccgcgc atcatcggtc accgcatcga aaaaccgcgt gccacccagc agtccgtctt
     6181 ccttcagacc cggagccaga tagcgcagtt gctgcaacgg gtagtacaga tggttgcgct
     6241 tgccagccag ggcgtcgtac accgccagca agctgccaaa taccttgggg ccaggaaagc
     6301 cgccctggta atgggccatg ataaaactta aggggtcgac cagccccggc gcttcctgca
     6361 gcaggcgctg gcgctcgcgt accgaatccc gggtcagggc gaattggccc ttggcgatat
     6421 aacgcagccc gccatgcacc atcttcgagg agcgactgga ggtgccccag gcgaagtcgc
     6481 gctgttccag caacaggcat ttccagccgc gccgcgctgc ttctcgcaga attccggcac
     6541 cgctgatacc gccgcccacg actatcaaat cccagtcgcg tgcggccagc tcgggcaagg
     6601 cctgttcgcg ccaggcggca ttccagttgt tcatcatcaa tcctgaatca gcacgccagg
     6661 tgccaggcgc tgctcaggat cgaaatgccc ggccaacgcc ttgagcgtgg ccatgcccag
     6721 ttcgcccttt tccaccggca gataaggcgc gtgatcgcgg ccgacaccgt gctgatggct
     6781 gatggtgccg cggttctcgg cgatggtcag gctggcggcg tgcttgagct tctgccagcg
     6841 cgccatggcc tcggcgtagt tagcaccagg gcggaacaca taggtggtgt agatgctcga
     6901 accttcgttg tagacgtggg acaggtgagt aaacacatgc acctgctcgc cctcctcact
     6961 caggccattg cgcaggctgg tctcgacctt gttcagcaga ctgtcgacgt tgctccagtc
     7021 ggtggcggtt tccagggtgt ccaccgcata gccagcggcc cacaggccgt ggcgcaggta
     7081 gggaaaacgg aagcggtttt ctgcccactt cttgcccagc agagtgccgg taaatacgcc
     7141 gtcaaagctc ttcagcagct tcttcgcctg ctttagcgaa gcggcgttct gtgtgcggct
     7201 gcccgtgacg ccaaaggtca gcatgcattt gccgtcgcgg gcgccgcgca gggccaggta
     7261 tttctccagc caagcgatct gctgcggatg gccggccagg gccagctggg ttttggtttc
     7321 aatggcgttg gacaggcgca gcatcgacag cggtactcgc gcctgggcca ggctgcgaat
     7381 ggcgttcagc gcctgctgcc aattgggcag gaacaccgcg tagaaattct cctgatcggc
     7441 caggcgggtg atgcgcacct tgacctcgga aatgatgccg aagcgccctt ccgaacccat
     7501 caggatttcc cgcagatcgg ggcccgcggc agaagccggg aaagtcggaa tctccagggt
     7561 gccggcgaag gtctcgacct tgccgccggc gaacaactgc tcgatgcgcc cgtagcgcag
     7621 cgactgctgg ccgctggagc ggctggcgac ccagccaccg agggtcgaca gctcccagga
     7681 ctgcgggaaa tgaccgaggg tatagccctg ggcgtgcaac tggctttcca cctgcgggcc
     7741 agtggcgccg gggccgaagg tggcgatcag gctgtcgtgg tcgatctcga tcagcttgcc
     7801 catgcgctca agcgacaggg tcagcaccgg tttgctaccg gcctgcgggt tgatatgccc
     7861 ggccaccgag gtgccgccgc cgtaggggat gacaatcagg tcctgctggc tggcccaggc
     7921 cagcagttcg cggatctgcg cggcgctctc cgggtaggcc acaccatcgg ggaacacact
     7981 gaaatcgccg gaacgcatcg ccagccaatc cggcagactc tggccacggg catggcgcac
     8041 acggtcctcg gcgttcaggc agatcagcgg atgagcctgc agacgcgagg ccggaacctg
     8101 ggccagcacc tgctccaggc tggcatcggc cagcacctgg ccagggccga ccagttcggc
     8161 cagaaaagcc tcgccatggg ccggcagttc caccactgtt gcttcatcac cccagccgtt
     8221 ccaacgtcgc atctgtgtct cccggatatt tcagtcaccc tcgatggttg cctatactag
     8281 ctggccgccc aggagcgtca ctgtcgaatc gggccaggcg ctatcgccaa tcaagacatt
     8341 cagaataaga agatgaccga caacctcggc tacacctcag tcccctccct gctcaaatac
     8401 ctgcgccatg ccgagcagct ggggctggat atcgatcagg cattggcggc ggccggcatc
     8461 aaggccgagg acctggctga caacggcaag cgcatcccca gcgaaaccca tgaacgcctg
     8521 cttgcgcatt tgctgcgcgt ctccggtgat ccgctgttcg gcctgcactg cgcgcgtttt
     8581 gtgcagccag gctcctggag tgtgctgggc tatatcacca tgaactgcgc gaccctcggc
     8641 gaagccatga gccgcatcgt gccttatgaa aaactggtcg gcgatatggg cgtcagccgg
     8701 gtcgagaccg gtgcagacca cgtcaagatg atctggacct gccgccatca ggtcgaaccg
     8761 atccgccggc atatggtgga gaacgtgctg gcctcctggc tgctgtatgc acgctggatc
     8821 gccgacatgg accgctcgcc caatgaagtc tggtttgaac atgcgcagcc tgaaggcgcc
     8881 gtgctggccg attatcagac gattttcggc tgcccgattc gcttcgagca aagctgcaat
     8941 gcgctgatcg tgccgctgga atacctcggc gtgccgttgc gccaggccga cgccaacctg
     9001 ttgcgcaccc tggaagaaca cgcgctgacc ttgatggccg ggctggacga caacgagccg
     9061 ctgccgcaac gggtaaagaa cgccctgcgt ctgctgctca aggatggttt gccgcgcaag
     9121 gagcgggtgg cggagaaatt caatatgacg gtgcgcaccc tgcagcgtca tctgcagctg
     9181 gccggcacca gctaccagca gattctcgat gagctgcgcc aggagctagc cgagcattac
     9241 ctgctgcgca gcgacctggc gattcaggat atcgccagct acctgggctt taccgagtcg
     9301 cgctcgttcc accgcagctt caagagctgg accgggcaga ccccgggcga attccgcgaa
     9361 agccaacgta attcgtagga tgggttagcc gcaggcgtaa cccatcatca gacagcatgg
     9421 gtatcgctgc gctcaaccca tcctacatgg ccctagccga ggatttcgct tagcgggata
     9481 aacctcaggc tatcaccctc gaccagggtc gtaccctcca gcacctcggc aaaaccctca
     9541 gcccaggccg cgctgcgcaa caccccggag ctctgatttg ggtaaggcac cacgcggcca
     9601 ttttccaggc gcgcgcgcag gtattcgcgg cgattacccg gcttgctcca ggtaaaaccg
     9661 gccggcagcg gaaagcccaa aggctcgacc cgctgcacgc ccaaccggcg cagcagataa
     9721 ggccgcgcaa gcaggccaaa ggtcaccaaa gtcgaggccg gattgcccgg caggccgatc
     9781 accggcacgc cgcgaaactg cccgcaggtc agcggttttc ccggtttgat cgccagtttc
     9841 cacagcgcca gttcgccctc ttcacgcagg gccatgccga ggaaatccgc ctcgcccacc
     9901 gacacgccgc cggtggagag aatcaggtcg acatcgctca gggcagccag cgccgcgcgg
     9961 gtcagctcca ggtcatcgag cagaatgcct ccatccacaa cctcgcaacc cagacgctgc
    10021 agccaggcaa tcagcagacg acgattactg ttgtagatct gccctgggcc gagcggctgg
    10081 cccggctcga ccagctcatc accggtggac aacaccgcca cgcgcggccg tcggcgcacg
    10141 ttcaattcgg cgcagcccag ggaagcggcc agacccaatt caatcggacc taaacgagtg
    10201 ccagccggca gcacggaatc gccgatacgg gtttcctgcc cctgggcacg cacgttctgt
    10261 ccgagcttta gcggctcgct aaaggcagca cggccatcct cgccgagcac ggcgttttct
    10321 tgcatttcca cgctatcggc accggccggc aatggtgcgc cggtaaagat tcgtgcacag
    10381 gtgccgggct gcaaaggcgc aggcgcgcta ccggcctgga tacgctgact gacgaccagc
    10441 ggctcgccgt tccaatcggc caggcgcaag gcgtagccgt ccatggcgct gttgtcccaa
    10501 ggcggcagat ccagggtggc gatcagtggt tcggccagca cacggccaga agcgtcgctc
    10561 agcttgaccc actcgcattc aacaatcgcg ctgacctcgg cctgcgccag caagcgcgcc
    10621 agcgcggcct caacgggcat cagcccaggc ttgtcacagc cgctcatcca cggctctcgc
    10681 aggcggccac cggcttcaaa tgcggaacga aattgcacgg ccgatgacgg ttatccagct
    10741 gctcaccgag gatgccatcc caggcggtgc ggcaggcgtt ggtcgagccc ggcaggcagc
    10801 agaccagggt accgttggcg agacctgcca gagcgcggga ttgaatggtc gaggtgccga
    10861 tatcaggcac ggaaatctgc cggaacagct caccaaaacc atcgacctgt ttgtccagca
    10921 gacagctcac cgcttccggg gtgctgtcgc ggccagtgaa gccggtgccc ccggtaatca
    10981 gcaccacctg cacctgatcc tcggcaatcc aggtggcaac ctgggcgcga atcttgtaca
    11041 gatcgtcctt caacaaaacc cgtgcggcga gttggtggcc ggcagcctgc aggcggtcga
    11101 caaacagctg gccggaggta tcggtgtcca ggctgcgggt atcactgacg gtcaacaccg
    11161 cgatattcaa gggcacgaaa tgtgcctcgg ccttgtggtt catggcaggc ttccagttat
    11221 gggcgataat gcccgccgtt atatcacagg cccgctttcg gagactgcgc atgcccgccc
    11281 ccgccaccct gccacgctgt tctgttttgc tgcttgctgg cggccgcggg caacgcatgg
    11341 gcggtgctga caagggcttg ctcgactggc aaggccgccc gctgattgcc tggctgcacg
    11401 agctggtgcg accgctaacc gacgacctgc tcatctcctg caaccgcaac gccgagcgct
    11461 atgcgccctt cgcagatcgc ttggtcagtg atgacgaggc ggacttccaa ggcccgctgg
    11521 caggcattcg cgccggcctg cgcgtcgcac gccaggcgaa tgtattgatc ctgccgtgcg
    11581 acgccccaca actcgacaga ccgctgctgg aggcactact gagtctggcc ggtgagagcg
    11641 cagtcgtggt gcgtcagggc gagtttctcg aaccgctgtt cagcgtcatc ccccgtgcac
    11701 tgcatgctga cctggagcag gcctggcaag ctggcgaacg cagcccgcaa cgctggttgc
    11761 gcagccttaa tccattgaca gtggagtgcc cactcggtga ccctcgcctg gccaacttca
    11821 acacgcccga attgctgacg acacgcggat aaacaccggc aagggaactt gaccggcaaa
    11881 aattcgtcac aagcagggta aaaacctcta tccaatcaag gagaaaccca tgtctcagcg
    11941 gattattcca gcagtgctgc ttaccttggg cctggtcgcg ctgactggtt gcgcgtcgcc
    12001 aactgtaatt accctcaatg atggccgcga aattcaaacc atcgacgagc cgaattttga
    12061 cgaggattcg ggcttctacg agttcgaaca actcgatggc aaacgcgcca aggtcaacaa
    12121 ggatcaagtg cgtaccgtca aagagctgta agccgataac ccaggcgttg ccacgtacta
    12181 cttgcccagc gcctgggcgc tgatcgatat attcaaagtg cttggccaat agacaaaatt
    12241 aaaacctaac cccttgtgcc gcaaaacttt ctcagtaaaa ttttgaccca ttcggagtgt
    12301 agcgcagctt ggtagcgcgt ctcgttcggg acgagaaggt cgcaggttcg aatcctgtct
    12361 ctccgaccag atcacacaaa aaagcccgct taatcgcggg cttttttgtg tctgtgagaa
    12421 aggctattgc aagctttcga acgcatgcac cagggccttg ctcaggtcca cggtggagaa
    12481 cttggtcagc acatcattgg ccccgaccgc cttggctttt tcggtgttca tggtgctgtc
    12541 gagcgaggtg tgcagcagca catagatctg gccgatatct ggtttcttgc gcagcgtgcg
    12601 cactaggtca tagccgtcca tttctggcat ttcgatatcc gataccagca cctcgatggg
    12661 ctccccagca tcgaattgcc gctgcagcaa ctcaagggcg tcagcagcgc tgcgcaccat
    12721 ctggcaatcc agctccagct tgcgcagggt aatcatggtc tggtgcagcg cgacgtggga
    12781 gtcatccacc accagcacgc gacgcccctt gagcagcgca cgttcgcgct cggacaacag
    12841 ctgctcgtcc agctcctcga taaccatcgg cgcgagttcg tgcagcactt tttcgatatc
    12901 gagaatctgc acaatcttgc cgtcgatctg cgtcacccca gtgataaagg ctttgctgcc
    12961 ggaaccagca ggcggcgggc ggacatcgcg ggtgtaacac tgcacgatgc gctccacact
    13021 ttgtacgtgc aagccctgac gcgaacggct gagttcggta acgatcaggc aaccgtcgtt
    13081 gttacccggc acgccctgct cgccgatagc cttgcccaga tcgatcaccg tcagtgccga
    13141 accgcgcaga gtcgccacgc cacgcacatg aggatggcgg ctgggcagtt tggtcaacgt
    13201 cgggcaggga ataatttcct gaaccttgag cagattgatc cccagcaagc ggccgctgtg
    13261 caaacggaac agcagcagcg acaaggcgtc tccaagcacc atgctcattg atcttcttcc
    13321 ttcaataaat cattgaccgc gcgcaccttg agcatcaggc gctacgcagg aattcgacaa
    13381 tctgttcggc ttcgaatggc cagtccaact cggccccggt atcgctgcga cgcagcaccg
    13441 gaatgcgcag agcatagtcc tcgacccagt gttcatgctc agcaatatcg accagctcga
    13501 ccagcaggcc gtgctcgaca aaaggcatca gcaacgcttc ggccacctcg cacaggtggc
    13561 agccaagcgt accgaataat tggcattccg ggagcatctg cgcacctcca ctcactactt
    13621 gaggtgcagt ttagcactgc cccaggctca ggcgtccgtg gacttgctgc tgtctagggc
    13681 gggattgagc aggttctgca ggccatcgag caagcggctg ttgagctgct cggccttgtc
    13741 caggcgacca ctgtcaaaac gctcatccag cgagaagccg cctttgagca gatccagcag
    13801 gctggtttta gctgtgcctt cttcagccac ctgattggcg ctgcgcagtg cttcgagcaa
    13861 gccctgggcg tagtcgatca acgagctgtt gactgcgctg gcagcctggc cgccgtcttg
    13921 cgccacagca ctgtaggcat cggtagcctg gcgcaccgtg gtctgggtca aacgcaggga
    13981 catcgacgcc aactgctcgc catccatctc caacgccatg gctcggtcga aggcgccgga
    14041 tagatcgccg gcatagaact tgtctgacag gtcctgcacc tggccgaaga gtttttccag
    14101 cgcagcgcgc tcttcgtcat ccagctcgcc ttcaacgctg acctgccagg caccgatctg
    14161 caggctaccg gattggctac tggcaactac tgagctgccg ttgtcattgt tgctggcaac
    14221 aacaccgctc tgcgaccagt tggccgaagc ctgggcgatg gatatacgca ggcgatcacc
    14281 atcacgggtg gtcacttgca tctcgaaggt ttccgcttgg gcggcaaacc gctcgctgta
    14341 ggcggcgata ttggttgagc cctcaaccag cgccgcatcc ggattaaagc gtttgtccag
    14401 gtcgctgaaa ccgctctgga gtttctggta ggtgtcgtcg atatcactcg ccaccttgcc
    14461 ttgcagcacg cccatgccgt cgagaatctt acgcgcttcg gcaaaaccct tttccacgcc
    14521 gctgcgggcc tgttcaagca agcccttgag tttgcttggg tcggcaccag ctgcttgctc
    14581 gctttgcagg cgctgctcga taaaaccgag aatacgcccg gcgactttct ccggggtgta
    14641 atcattagcc ttaccggcca gtgcacccgg ttccagcccg aggcgttcag ccagacggtt
    14701 ggccagtgtc acctgcgcat cagcagcatt acgcacagcg ggctgagcgg taacggaagt
    14761 gggacgtgaa gcggaggata aatttgcggc cagagggttc atggcaaagt tcctaggcgg
    14821 tctatctgac tggttggcgg ccgtgagcag tgaaacttta gcctaacggc gaacaacccg
    14881 atcaacggca cgctacccag actagagcga ttgcaccggc atcctgcgcc caccaggcct
    14941 tagcggactg cgcacgagca gaccaaagtc taaaagccaa gcataagcct ccatgcttta
    15001 ttgcgcctcg ctgtaataga tcggccacaa gccgacccgc acacacgagg agctaacaat
    15061 aatgactcaa tctcccctcg cccgcgctgt ggcactcgcc acactgggca cctgcctcac
    15121 cctgccatcg ctggcacagg cagagtttat taaggacagc aaagccagcc tcgagctgcg
    15181 caatttctat atgaaccgcg acttccgcga tcctggtgca gcccaatcca aacgtgaaga
    15241 atgggctcaa ggctttcttt tcaaagctga gtcaggtttc acagaaggca cagtaggctt
    15301 tggtttggat gcctacgcgg ggctaggttt gaaacttagc tcagatgacg accttgcagg
    15361 gaccaaccta ttacccaatc gcccgggaga tgaaggcccc aataattaca gcgaagccgc
    15421 tgttgcagtt aaggcgaaac tatcaaacac cgtgctcaaa gctggtggtt tgatgcccaa
    15481 gttaccgatc gtatcctccg gtgactcacg cctgctgccg caagtttttg aaggtgcaat
    15541 gatcagctct caagaaatcg ctggtttatc ccttaatgcg ggccagttac gccaagtcaa
    15601 cgagcgtgac tcaacagata ggcgcgatat cagggcttca aattacacat ctgctggaaa
    15661 ccctcgcggc agcgaccgct acaacttcgg cggcggcacc tattcgttca atgcaggcaa
    15721 cacgactgca ggcctttggt acggtgagtt gcaggatgtg tacgaccaaa agatgtacaa
    15781 cctgattcac acgcagccca tcggagattg gaagctgggc gccaacctgg cttacttcga
    15841 ctcatccgat aatggccgca agctggcagg ccaaatcgat aacgacctta cctcgctgat
    15901 gctttcagcg ggcatcggtg ctcatacctt ccgcctggga tatcaagaca gtagtggtga
    15961 taacgcattt ccgttcatcg ccgaaactga tccggcgatt gccaactatg tgcaaatcct
    16021 tgatttcacc cgcgctgacg agcagtcctg gcaagcacgt tatgacgtca atttcgccac
    16081 ctacggcgta cccggcctaa ctgcatttgt acgctacatc aatggtgatg gatttgatct
    16141 tgctgatggc agcaatggga aagagtggga acgcgaccta gacatcactt acaccatcca
    16201 ggaaggcaca ttcaaaaacc tagctttccg ctggcgcaat gcgatggtgc gttctgatgt
    16261 cctgggcgac ctcgacgaaa accgcctgat cgtcagctac accctgccgc tgttctaagc
    16321 ctcaacactt aagcggtcgc aataaaaaag gcccggacac tgtccgggcc ttttgcatta
    16381 gcacgtagcc atcaatcctg gctgacggcg ccaatcttgt gcaccgagag gtcggcaccg
    16441 tagtactcct cctcctggct caggcgaatg cccacggtag ctttcagcac accgtacacc
    16501 agcacgccac caatcaaggc caccaccacg cccagtgcgg tgccgagaaa ctggctggcc
    16561 aggcttacac cgcccagacc gcccagcgcc tcttggccga agatgccgca ggcaataccg
    16621 ccccacaccc cgcacagacc atgcaacggc catacgccga gcacatcgtc gaccttccac
    16681 ttcacctggg tcgcagtaaa cgcccagacg aacaacgcac cggcaatggc acccgtgacc
    16741 agcgcaccga ccggatgcat caggtcagaa ccggcgcaca ccgccaccag accggccagc
    16801 gggccgttgt gcaggaagcc agggtcatta cgcccaacaa ccaaggccgc caccgtaccg
    16861 ccgaccatcg ccatcagggt gttgaccgcc accaggccgc tgacgctgcc cagggtctgt
    16921 gcgctcatca cgttgaagcc gaaccagccg atgatcagaa tccacgagcc cagcgccagg
    16981 aacggaatat tcgacggagc aaaggccacc agacgaccat cgcggtaacg cccatcacga
    17041 cgccccagca gaatcaccgc gccaaaggcc agccagccgc ccatcgcatg caccaccacc
    17101 gaaccggcaa agtcatggaa ggttgcgccg aactgcgcct ccagccagac ctgcacgccg
    17161 tagttgccgt tccagatcac cccctcgaag aaagggtaga taaaggccac gatcagcacc
    17221 gtggcacaca gctgcgggcc gaacttggcg cgctcggcga tgccaccgga aatgatcgcc
    17281 ggaatggccg cggcaaaggt cagcagaaag aagaacttca ccagcgcata gccattgcct
    17341 tcagtcagca cactggccgg ctcaaggaag gtcacgccat aggcgatcca gtagccgaca
    17401 aagaaataag ccagggcaga cacggcaaag tcggagatga tcttcgataa ggcgttgacc
    17461 tggttcttct gcctgaccgt acccacttca agaaaggcaa agccggcgtg catggccagc
    17521 accatcacgg caccgagcag gataaacagg gtgtttgaac cgtgaatcag ggtctccacg
    17581 gcactgttga tgttttccat cggcaaagaa aactccgtcg cggggaaaaa gcaccaagtc
    17641 ggatcacaag ccaataaatg cgcaccatat ggcagcactc atcatgatcc agtttgccga
    17701 gatcaaggtt gtacagcact aaaccggtgc tgattgagcc ttgcctcgaa gtacttattc
    17761 catagttatt aatgagttat tgatgcacca cggcgtcctg tccagaaccc ttgcagcccc
    17821 ataaatgagc acatccgcaa aggcgcgcac cagcgtaatg caagcactgt accagccgat
    17881 gagtcagcgg catccgcccc gcagcgaaga aaaaaggaac caccgccccg cagaggactc
    17941 gaacccccta cactgccccg tcacaaggag agacaccatg gctcgtaaga cccccgtatc
    18001 atccagctca gatcagctac tcgacgaatt ccaggcgctg gtgcgcgaca ccgaaagcct
    18061 gctgcagcac tccgccagcc tcgcaggcga acaagctgaa gagctgcgcg aacagatccg
    18121 cagcagtctg ggccgcgcac gcagcaccct gcacaacgcc gaagacgccc tgcacgcacg
    18181 cggcaaagcg gctcttgaag ccaccgaagg ctatgtgcag actcacccgt ggcaaaccct
    18241 cggcattgct gccgctatcg gtctgctgtt gggcatgctg atttcccgcc gttaaagaga
    18301 acatttaatg gatgcaacac cgcccacccc gccaagtcgc agcagctcgc cacggcgatt
    18361 tgccagcgcc ttactggggt tattacaggg ccatatcgcg ctatttggcg aagagctcaa
    18421 agaacagcaa agccatactc tcaacctact ggtactcacc agcctgagcc tgctgttcgg
    18481 cctgctgctg atcatcggct tgtccgccgc gctgctgatt gccttctggg agacacaccg
    18541 cctgctggtt atcagcgccc tgtgcgcgtt ctatgcgctg tgcatgctcg gtgccctgat
    18601 cacgctgctg caacgtgtgc gcaatgcgcc tgcgcctttc agcgccagcc ttgaagaact
    18661 ggcccgcgac cgtgagcaac tgctgccatg actatgcccg agctacccgc caaggcctcg
    18721 cgccgcgagc tacgcaaaac cctactgcgc atgcgtctgg aaatgcatcg ccaggaactg
    18781 cgccacgaaa ccctggtgat gctgcaaccg ctgcggcaag cgcaacactg gcgcagtcac
    18841 tggcgtgaag agctgggcaa tagcaacgca ccactctggg tcgctggtgg tgcactacta
    18901 ctggccacca ttggcatccg ccgtggccaa tggcgacgct ggctgcgtat cgcgctggtc
    18961 gccttcccgc tgctacggcg caacccagcg cgtccggaca cgccgacggg cacgactaaa
    19021 gatacccaag caggggatta aactggccac gatcttgctt agatactcag ctaacgccgc
    19081 ccacagggcg tcttgcctgg ctaaggaact gcgcagtgtt caacggtcaa ccgcttgctg
    19141 ttttccaacc cttcattgat accgccaccg gacgtattgc cggtgtcgag gcattggcgc
    19201 gcctgcgcag cgctgatggc cggttgcact cggcaggccc gttattcagt gacccgaaaa
    19261 ccccgccaac ggctctgcgc cgcctcgacc gtgaggtgcg cgagcaagcc ctgacgcgct
    19321 ttcatgaagc cccgcaggac tggtttctca gcctgaacat ctcgccacgc tggatcagcc
    19381 ggctgcgccc cgaacaagcc ctgcccagcc tcaagcagct gcaacgcagc ggcatcgacc
    19441 cggcacgtat tgtttttgag atcaccgaac tgggcggcgc cagcacgcgc ctgcccggcg
    19501 tggtggaacg ctatcggcaa gccggcgccc gcattgccat cgacgacttc ggcgccggct
    19561 actcacaact cgaccgcgtg ctggcattgc aaccagacat cctcaagctg gacatgcgcc
    19621 tgtttcagca agccgctcgt ggtggaccaa gcagtgaagt ggtcaaggca ctggcacaga
    19681 tggcggaaaa gaccggctgc tggatcatcg ccgaaggcgt ggaaaccgaa gccgagctgg
    19741 actttgccct ggagtgcggt gcgcgctatg tgcagggcta cctgtttgca aaacccgaag
    19801 aagcgttctt cagcagccag gccttcgtca aacgctttgc ccagttacgc gaccactacg
    19861 tgcagcaaaa actcgccgaa cgtgcacgcc tgatgaccct gcgccagcaa ctcggcgagc
    19921 tgatgaacag cttcaaggcc tggagcgaag gcggcgcaca gcccgatcag ctgccacaac
    19981 cgcatgccta cccctggctg ctacgcatct tccaatgcga ccgctacggc acccagctga
    20041 ccgccaacct ggagtggcgc gaacacagct ggcgcagcga tacgcgctat gtcgggcata
    20101 actggtcgtg gcggccgtac ttctaccaac tgctggccga aggctgggaa gaacgccggc
    20161 tgattctctc cagcacgtac cgcgacgcca ctaccaacca gtactgcatg accgccgggc
    20221 aatttatcga caacggcaaa cggctgctgc tgatcgatat cgacgctgcc gggttttaac
    20281 aacacctcgc cttgcatcag cggcatagaa ccccgaagct agctgccaca gccaggtaac
    20341 ggcgaggaca acccttggat tggcaaaccc tgctcaaccg tgaacgactc ggcaagccga
    20401 cgcacagcgt cgccgaactg ggccgcaccc cgtttcacaa agaccacgac cgcatcatct
    20461 tctctggcgc gttccgccgc ctggggcgca aaacccaggt gcacccggta tcaagcaacg
    20521 accatatcca cacccgcctg acccactcgc tggaagtcag ctgcgtgggt cgctccttgg
    20581 gtatgcgcgt tggcgagatg atccgcgatg ccctgccgga ctggtgcgag ccaagcgacc
    20641 tgggcatggt cgtgcagtcc gcctgcttgg cccacgacat tggtaatccg ccctttggcc
    20701 actctggcga agacgccatc cgccactggt tccagcaggc ggccggacgc ggctggctgg
    20761 attcgatgag cgcggccgag cgcggcgact ttctcagttt cgaaggcaat gctcagggct
    20821 ttcgcgtact gacccagctg gaataccacc agtttgatgg cggcacgcgg ctgacctacg
    20881 cgaccctggg cacctacctg aaatacccct ggaccgcgcg ccacgccgag gcgctgggct
    20941 acaagaaaca caagttcggt tgctaccaga gtgagttgcc gctgcttgag caaatcgcca
    21001 gcaagctcgg cctgccgcag ctggaagaac aacgctgggc gcgtcacccg ctggtttacc
    21061 tgatggaggc ggcggacgac atctgctacg gcctgatcga cctggaagac ggtctggaaa
    21121 tggagctgct cgattacagc gaggttgaag cgctgctgct cgacctggtc ggcgatgacc
    21181 tgccggaaac ttaccgccaa ctcggcccgg acgactccaa gcggcgcaag ctggccatcc
    21241 tgcgcggcaa agccatcgaa cacctgacca atgccgccgc cgaagccttt gtcgagcagc
    21301 aggatgcctt gctcgccggc accttgcagg gcgatctggt cgagcatatg cacggcaccg
    21361 ccaagcgctg cgtacagagt gccaaggaca tggcccggca gaagatcttc caggacaaac
    21421 gcaaaaccct gcatgagatc ggcgcctaca ccaccctgga aattctactg aatgccttct
    21481 gtggcgcggc actggagcag catggagggc gcacgccctc cttcaagaac cggcgcattc
    21541 tcgacctgct cggcaataac gcacccgatc ccaactggcc gctgtaccgc tcattcctgc
    21601 gcatgatcga ttttatcgcc ggcatgacgg acagctacgc tactgaaatg gcccgcgaga
    21661 tgacgggccg ttcaagcccg gtataagcca tgaatagctt cctgcgacaa accttcagct
    21721 ggcatgccgg cccgcctgcc tggggcccgg cgatggtcgc cggcttcggc tgtgcgctgc
    21781 cattgctgct tgggctgttc agcgctcatc caggttttct ctgggcctcg gcgggcgcct
    21841 ttcaggcggc ccaggccaac ccgctgcatc gcctgggcat gctgcgcatg ttgatgctct
    21901 gcggactggg cgcctgcagc gccgggttgg gcttctggtc cgccgagcat gcctacgcaa
    21961 gtttgctgat cttcgcagcg ttcggctttc tgctcgcctg gctgcagcgc tttggcagcg
    22021 aagccggcaa gctgggcatc ggcctggcca tctgcctgtg cctgggccaa ggccagcagg
    22081 gcctgggctc gctgaacaac ccctatgcca tcgccatgct gtttatcctc ggtgggctgt
    22141 gggtgatgct gctggccttc ggtctgcgtg gcatgcatgg cctgcgcatg tggccgtaca
    22201 tgccgcgctt tatcagcatc ttcaaggtgc tcaaacgcca cgccaaacgc ctgccgcaac
    22261 agaagtggcg cctgcatgcg ctgggctgca ccctcgcgtt cgccctctcc gggctgatcg
    22321 tcaacctggc cgaactgcac cgcggctact ggctgacgct gaccgtggtt accaccctgc
    22381 agctgaattt ccagggcagc ctggtgcgcg ccgtgcaatc gattcttgcc agcctaggcg
    22441 cagccgcgct gctgattctg ctcggccata gcatgcagga tccggcgctg atggtcgcca
    22501 cattgctacc gctgatcgcc cttagccgcg ccctgcaggc caaccattac ggcctgtttg
    22561 tgctgcagac gacggtatgc ttcgtattgc tggcagaaag cttggcgcat gactggcagc
    22621 tgccccaggt gcgcctgttc aacagcctga ttggcgtggc ccttgccctg ctggtagcac
    22681 tgctggtgta tggcctggag cgctggttga cgcatcggca caatttgcac aaagccaagc
    22741 acgccacaga cccaatttag agctgccgca gccgcgcaaa agctgcaccc gcgcaacggc
    22801 aaagcgcgta aggctccact atgctttgcg cttagtcagg gcatacaagg gcatgcgcgg
    22861 atgcagcaga cagcaaggtc tcaggcaaag tccaaggaac gacgtttccc gcttcatatc
    22921 catatcagcg tgctcttcac cctgctgctg ctgggctcgg gcgtggtgct gggatacttc
    22981 aactatcagc agaccagcaa catcattctc agcagcagcg atcagctgtt cagccagatg
    23041 cgcaaggaag tccaggcgga tctgcgcaat acctaccaac cgattcgcta catgatcaac
    23101 ctgctggcgc gtaacgagca gatcaacggc cgtgaccgtc atgagcggat gatcatgctg
    23161 ccgattttca gccaggcgct gcgcgacaac cccaagctgg cgtcgatcta tgtgggctat
    23221 ggcgatggca gtttctttat ggtgcggccg ctgcgcagcg aaaaactgcg acagattttc
    23281 ttcgcccccg ataatgccga gtatcaggtg tg
//
LOCUS       DASRUM010000230         3679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2443632, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000230 DASRUM010000000
VERSION     DASRUM010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2443632"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VFF22_15470"
     CDS             <1..459
                     /locus_tag="VFF22_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HZX18249.1"
                     /translation="RGMIRQHQFDKVEMVQIVEPSKSFEALESLTANAERVLQLLELP
                     YRKLALCTGDMGFGAVKTYDLEVWVPSQDKYREISSCSNCGDFQARRMQARYRNPETG
                     KPELVHTLNGSGLAVGRTLVAVLENYQQADGSIRVPDVLKPYMGGIEVIG"
     gene            676..2070
                     /gene="cysG"
                     /locus_tag="VFF22_15475"
     CDS             676..2070
                     /gene="cysG"
                     /locus_tag="VFF22_15475"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011062163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HZX18250.1"
                     /translation="MDFLPLFHKLQDRLVLVVGGGEVAVRKARLLSDAGATLRVVAPE
                     IRDELREMASARNGEVLLRDYQSADLHGVALIIAATDDEPLNAQISAEAQALGIPVNV
                     VDAPKLCSVIFPAIVDRSPLIVAITSGGDAPVLARLIRAKIETWIPATYGQLAGLAKR
                     FRAQVKGLFPDVQQRRVFWEDVFQGQVAESVFAGKLQEGQRLLEEKIAGIAPRTLGEV
                     YLVGAGPGDPDLLTFRALRLMQQADVVLYDRLVAPAIIELCSRDAERIYVGKQRAEHA
                     VPQEQINQRLVSLAKEGKRVLRLKGGDPFIFGRGGEEIEELAAHGIPFQVVPGITAAS
                     GCAAYAGIPLTHRDHAQSVRFVTGHLKDGSSNLPWNELTASSQTLVFYMGLVGLPIIC
                     QQLIAHGRAATTPAALIQQGTTQNQRVFTGTLANLAELVAEHEVHAPTLVIVGEVVQL
                     REKLAWFEGAQASV"
     gene            complement(2055..3041)
                     /locus_tag="VFF22_15480"
     CDS             complement(2055..3041)
                     /locus_tag="VFF22_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015372401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HZX18251.1"
                     /translation="MNLVTPAEHPFAQFVRALGKGKRGARNLTREEAREAMGMLLDGK
                     VEDTQLGAFLMLLRHKEESAEEMAGFAEAVRARLQAPAIKVDLDWPSYAGKKRHLPWF
                     LLAAKALAGSGIRILLHGGGPHTAGRMYSEQLLDTLHIPLCRNWGSVEQALAQQQLAF
                     IPLGDWMPQLQRMIDLRNILGLRSPIHSLARILNPLGARCGLQSIFHPGYQAVHREAS
                     QLLGDSALVIKGEGGEIEINPDATSHLYGTANGENWDEEWPALSALRHVKPESLEPAH
                     LAAFWRGEVADDYGKLAVISTMALALRALGEPREQALDRAAQLWAARNQTLA"
     gene            complement(3038..3373)
                     /locus_tag="VFF22_15485"
     CDS             complement(3038..3373)
                     /locus_tag="VFF22_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HZX18252.1"
                     /translation="MTTLNVEGRSLALDKDGYLLDLADWSEATAHALAQREGINLSAE
                     HWEILWLVRSFYDEFQLSPANRPLIKFIAVKIGPEKGNSLNLNRLFNGAPAKLAAKLA
                     GLPKPTNCL"
     gene            complement(3370..3639)
                     /gene="tusB"
                     /locus_tag="VFF22_15490"
     CDS             complement(3370..3639)
                     /gene="tusB"
                     /locus_tag="VFF22_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HZX18253.1"
                     /translation="MATLHVLSHSPFTDSRLDALLLCGDAVYALQAGSAQRQALELMP
                     ESIALYALDEDVQARGLMFPSRLTLVDYSAFVALSCQYDKVNTWL"
BASE COUNT          716 a         1074 c         1139 g          750 t
ORIGIN      
        1 cgcgggatga ttcgtcagca ccagttcgac aaggttgaga tggtgcagat cgtcgagccg
       61 agcaaatctt tcgaagcgct ggaaagtctg accgccaacg ctgagcgcgt gctgcagctg
      121 cttgagctgc cataccgcaa actggcgctg tgtaccggtg acatgggctt cggcgcggtg
      181 aaaacctatg acctggaagt ctgggtgccg agccaggata agtaccgcga gatttcctcg
      241 tgctctaact gcggcgactt ccaggcgcgt cgcatgcaag cacgctaccg caacccagaa
      301 accggaaaac ccgagctggt gcacaccctc aacggctcag gcctggcggt aggacgcact
      361 ttggttgccg tgctggagaa ctaccagcag gccgatggca gcatccgcgt gccggacgtg
      421 ctgaagccct atatgggcgg tatcgaagtc atcggctaac aggccgttga aaaactacct
      481 gcgttggcaa tactgcgtta aaaacaggct cggaatgctc atttacaacc cgtaaactcc
      541 gcttcctcgc ctgtttttgc cttgccttgc ctgcctctgc tacgtttttc aacggcctga
      601 taagccggct tcagttacct gcgacggggc ggccagtgct gccccgtttt gtttacccga
      661 ttgagagcat gcgtcatgga ttttctgccc ctgttccaca aactgcagga tcgtctggta
      721 ttggtggtcg gtggcggcga agttgccgtg cgcaaggcgc gtttgctcag tgatgccggc
      781 gcgacgttgc gggtggtggc gccggagatt cgcgatgaac tgcgtgagat ggccagcgcc
      841 cgtaacggcg aggtgctgct gcgtgattat caatcggctg atctgcacgg cgtggcgctg
      901 attattgccg ccacggatga cgaaccgctg aatgcgcaga tatccgccga ggctcaggct
      961 ttgggtattc cggtcaatgt ggtggacgcg ccgaagctgt gcagcgtgat tttcccagct
     1021 atcgtcgacc gctcgccgct aatcgtggcg attaccagcg gtggcgatgc accggtgctt
     1081 gcacggctga ttcgcgccaa gatcgaaacc tggattcccg ccacctatgg ccaactggcc
     1141 ggcctggcca agcgctttcg cgcccaggtc aaaggactgt tccccgatgt gcagcagcgc
     1201 cgggtgttct gggaggatgt gttccagggc caggtagccg aaagcgtctt cgccggcaaa
     1261 ctgcaggaag gtcagcgcct gctggaagag aaaattgccg gcattgcccc gcgcaccttg
     1321 ggtgaggtgt atctggtcgg cgctgggccg ggtgatcctg atctgttgac cttccgtgcg
     1381 ctgcgcctga tgcagcaggc cgatgttgtg ctgtatgacc gcctggtggc cccagccatc
     1441 atcgaactgt gcagccgtga tgccgagcgc atttatgttg gcaaacagcg cgccgagcat
     1501 gccgtaccgc aggagcagat caatcagcgc ctggtcagct tggctaaaga aggcaagcgg
     1561 gtattgcgtc tgaaaggcgg cgacccgttt attttcggcc gtggcggcga ggaaatcgaa
     1621 gaactggccg cccacggcat tccctttcag gtggtaccgg gcatcaccgc agcctcaggc
     1681 tgcgcggctt atgccggcat tccgctaacc caccgcgatc atgcgcagtc ggtgcgcttt
     1741 gtcacaggtc acctgaaaga tggcagcagc aatctgcctt ggaatgagct cacggccagt
     1801 agtcagaccc tggtgttcta catgggtctg gtgggcttgc cgatcatctg tcagcaactg
     1861 attgcccatg gtcgcgccgc aacaacccca gcggcattga tccagcaggg caccacgcag
     1921 aaccagcggg tgtttaccgg caccctggcc aatctggccg agctggtggc ggagcatgag
     1981 gtgcatgcgc cgaccctggt aatcgtcggc gaggtggtgc agctgcggga gaaactggcc
     2041 tggttcgaag gggctcaggc cagcgtctga ttacgcgcgg cccacagctg tgcggcgcga
     2101 tccaaggctt gttcgcgcgg ctcacccagg gcacgcaagg ccaaagccat ggtgctaatt
     2161 acggcaagct tgccgtaatc atccgccact tcaccgcgcc agaaggctgc cagatgtgcc
     2221 ggttccaatg attcgggttt gacgtgacgc agcgccgaca acgccggcca ctcttcatcc
     2281 cagttttcgc cattggccgt gccatacagg tggctggtgg cgtccgggtt gatctcgatt
     2341 tcaccgcctt cacccttgat caccaaagcg ctatcgccga gcaactggct tgcttcacgg
     2401 tgcaccgcct gataacccgg atgaaaaatg ctctgcaggc cgcaacgcgc gcccagcggg
     2461 ttgaggatgc gcgccaggga atgaatcggt gagcgcagac cgaggatatt gcgcaggtcg
     2521 atcatccgct gcagttgcgg catccagtcg cccagtggga taaacgccag ctgttgttgc
     2581 gccagtgcct gctcaacgct gccccagttg cggcacagtg gaatgtgcag ggtatcgagt
     2641 agctgttcgc tgtacatgcg cccggctgtg tgcgggccgc cgccgtgcag caggatgcga
     2701 ataccgctac ccgccagggc tttggcggcc agcaggaacc agggcaggtg acgcttcttc
     2761 ccggcataac tgggccaatc cagatccacc ttaattgccg gcgcttgcag gcgcgcgcgt
     2821 acggcttcgg caaagccggc catttcctcg gcgctttctt ccttgtgccg caacagcatc
     2881 aaaaaggcac ccaactgggt gtcttcgacc ttcccatcaa gcagcatgcc catggcttcg
     2941 cgggcttcct cgcgagtgag gttgcgtgcg ccgcgcttgc ccttacccag agcgcggacg
     3001 aactgggcaa aagggtgttc ggctggtgtt acaaggttca taggcaattg gtcggcttgg
     3061 gcaggcccgc cagcttggcg gcgagtttgg cgggagcgcc attgaacagg cgattgaggt
     3121 tcaggctatt gcctttctct gggccgattt tcacggcaat aaatttgatc agcgggcggt
     3181 tggccggtga caactgaaac tcgtcgtaaa agctgcgcac cagccagaga atttcccaat
     3241 gctcggcgct caggttgatg ccttcacgtt gtgccagggc atgcgcggta gcttcggacc
     3301 aatcggccag atcaagcaga tagccgtcct tatccagcgc caggctgcga ccttcaacat
     3361 tcagcgtggt catagccagg tgtttacctt gtcgtattgg cagctcagtg caacgaaggc
     3421 ggagtaatca accagtgtga ggcgggaagg aaacataagg ccgcgagcct gcacgtcttc
     3481 atccagggca tacagcgcaa tgctttctgg catcagttcc agcgcctgac gctgcgcgct
     3541 gccggcctgt aaggcatata cggcatcacc acaaagcagc agagcatcca ggcggctatc
     3601 ggtaaagggc gaatgcgaaa gtacgtgcaa ggtagccatc agagcgtaat cacctggtcg
     3661 taacggtcaa tcaaggcgc
//
LOCUS       DASRUM010000231        10759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1460106, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000231 DASRUM010000000
VERSION     DASRUM010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10759
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460106"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VFF22_15495"
     CDS             <1..446
                     /locus_tag="VFF22_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HZX18254.1"
                     /translation="FAPAGNFVLRESAKPLVLITAGVGITPALSMLEAARDTGRDIHF
                     IHCARHAGVHAFRDWVEAQGDKRPQLRHYVCYSEPREGDAGDAEGFLSLEQLAEWLPE
                     QRDLDAYFLGPKPFMAQVKRHLHSLGVPTAQSHYEFFGPASALDS"
     gene            469..723
                     /locus_tag="VFF22_15500"
     CDS             469..723
                     /locus_tag="VFF22_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane sensor/regulator PpyR"
                     /protein_id="HZX18255.1"
                     /translation="MLAYLSCPSRVLQLANRMLLAGVLLLLSGLLGAYGLEAQLSMGS
                     LVTAHSLTIIGPGLLKLGYVLRLAAQQHLRKQQEPCCAVA"
     gene            710..1900
                     /locus_tag="VFF22_15505"
     CDS             710..1900
                     /locus_tag="VFF22_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018927794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HZX18256.1"
                     /translation="MQLRDRQQELAIAPLWRLGFRPFFLGGALFAVLAIGLWVAVLLG
                     YLPGWQPVGGSLAWHRHELPFGFAVAIIAGFLLTAVQNWTGRPGLSGWPLAALFGLWL
                     LARLAWLLGAPLPLLLALQLPFLPLLALLLGRQLWQVRQWRNYPLVVVVLLMAGCEWL
                     LLYGLALGDDALQRRGALAGLWLIAALVCIIGGRVIPFFTQRGLNRAEPAAPRPRLDL
                     LVLVLSLSLALLFASGLALQPSVWMLPLLLVLVMLHGLRLQHWYDGGIWRVPLLWSLH
                     LAYAWLPLALLALVAWHAGWLSNASLAYHALALGTVGGAILAMLARVSLGHTGRPLQP
                     PALMGWAFASLQLAVVARVLLVPFAPGTGLGLSSLLWIVAFALFLRHYASMFWQARVD
                     GQPG"
     gene            complement(2000..2896)
                     /locus_tag="VFF22_15510"
     CDS             complement(2000..2896)
                     /locus_tag="VFF22_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZX18257.1"
                     /translation="MFTRTDLAFTSHGITCKGYLYMPADAIGPVPCVIMGHGFAATRE
                     CGIAPFAEAFANAGYAVFMFDYRHFGASDGQPRQLLVPSREVEDWLCAIAFVRKLNGI
                     KPDAIALWGTSFGGGLVTVAAARDGNVQAIIAQCPMMDGLASVLAVTRYAGVAHVLKL
                     SAHGALDITRALLGMSPHYIASAGRPGAVAAMTAADCYDGYLALIPEGVPNTVAARVS
                     SSLLAFRPVTEAHKVTCPALIQICEKDTVAPVAAAEKAAKKMKHAEVRRYDMGHFDIY
                     QGENREKSLADQLAFLLKTFSR"
     gene            complement(3271..4494)
                     /locus_tag="VFF22_15515"
     CDS             complement(3271..4494)
                     /locus_tag="VFF22_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZX18258.1"
                     /translation="MPGALSHIRVLDLSRVLAGPWAGQILGDLGAEVIKVERPGSGDD
                     TRHWGPPYVKDAEGNDSREAAYFQSANRNKQSVTLDFTQPEGQRLVRELVQNCDVLLE
                     NFKVGGLATYGLDYASLKAINPRLIYCSITGFGQNGPYSKRAGYDFMIQGLGGLMSLT
                     GKPDGEEGAGPIKVGVALTDILTGLYATVGVLAALNQREQSGLGQHIDVALLDVQVAC
                     LANQAMNYLNTGVAPKRLGNAHPNIVPYQDFPSADGNFIIAVGNDGQFRKLCEVAGCA
                     ALADDPRFASNKARVTHRAELIPLLRQATVFKTTAEWVTLLEAAGVPCGPINDLAQVF
                     ADPQVQARGLRLDLPNSLGSTTPQVASPLRLSETPVEYRNAPPLLGEHTEQVLTRHLG
                     LSAEQINSLREAGVL"
     gene            complement(4584..5765)
                     /locus_tag="VFF22_15520"
     CDS             complement(4584..5765)
                     /locus_tag="VFF22_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZX18259.1"
                     /translation="MAGKASFNWIDPLLLDQQLTEEERMVRDSAQQFAADKLAPRVLE
                     AFRHEQTDPKIFREMGDTGLLGATIPAEYGGSGLNYVCYGLIAREVERIDSGYRSMMS
                     VQSSLVMVPIFEFGNEATKQKYLPKLASGEYIGCFGLTEPDHGSDPGSMITRAKKVDG
                     GYRLTGAKMWITNSPIADVFVVWAKDDAGEIRGFVLEKGWQGLSTPAIHGKVGLRASI
                     TGEIVMDNVFCPEENAFPDVRGLKGPFTCLNSARYGISWGALGAAEFCWHTARQYTLD
                     RKQFGRPLAQTQLIQKKLADMQTEITMALQGCLRLGRMKDEGTAAVEITSMMKRNSCG
                     KSLDIARMARDMLGGNGISDEYGVARHLVNLEVVNTYEGTHDVHALILGRAQTGLQAF
                     F"
     gene            6009..6917
                     /locus_tag="VFF22_15525"
     CDS             6009..6917
                     /locus_tag="VFF22_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX18260.1"
                     /translation="MRRKIPSTAALIAFESAARHQSFTKAAQELSLTQGAVCRQIAAL
                     EAFLNIELFRRSRRGVVLTEAGQSYARRVAGQLDAVERDTLAVMGQQGAMSIELAVVP
                     TFGTQWLLPRLKDFQRLHPEITVNLTNRTRPFLFADTEFDAAVYFGDGDWSGTQAHLL
                     MGENPMPVCSPALLAEQTQLSAEQIANLPLLQQTTRPYAWRQWFSAQGLNTSRDMAGP
                     RYELFSMLAQAAMHEMGVALIPPFLIRRELDEGRLVIAAPLSLPTDKAYYLMIPERKL
                     ESAALLLLRDWLVREAHVYALAHGLK"
     gene            7472..8593
                     /locus_tag="VFF22_15530"
     CDS             7472..8593
                     /locus_tag="VFF22_15530"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HZX18261.1"
                     /translation="MHIGVPLETHAGETRVAATPETIKKLIGQGHQVTVQTGAGVSAS
                     IPDSAYEAVGAAIGNDAAAFGADLVLKVVAPTDAELAHMKAGAVLVGMLNPFSNETIA
                     RINARGVTAFALEAAPRTSRAQSLDVLSSQANIAGYKAVLLAAHHYPRFMPMLMTAAG
                     TVKAARILILGAGVAGLQAIATAKRLGAVIEASDVRPAVKEQIESLGAKFVDVPFETD
                     EERECAQGVGGYARPMPASWMERQAKAVHERAKQADIVITTALIPGRKAPTLLHAATV
                     AEMKPGSVIIDLAAAQGGNCPLTVAEQVVVQHGVTIVGHSNLAALVPADASALYARNL
                     LDFLKLVIDGEGKFHLNLEDDIVAACLMCRDGNVVRTNG"
     gene            8608..8940
                     /locus_tag="VFF22_15535"
     CDS             8608..8940
                     /locus_tag="VFF22_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HZX18262.1"
                     /translation="MDMISDGIYNLIIFVLAIYVGYHVVWNVTPALHTPLMAVTNAIS
                     AIVIVGAMLAAALTVTPLGKTMGTLAVALAAVNVFGGFLVTRRMLEMFKKKAPKAAAG
                     AATKGENH"
     gene            8940..10376
                     /locus_tag="VFF22_15540"
     CDS             8940..10376
                     /locus_tag="VFF22_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HZX18263.1"
                     /translation="MSMNLITVLYLVASICFIQALKGLSHPTTSRRGNLFGMIGMGLA
                     VLTTVGLIYKLGAEIATQGIGYVIVGLLVGGTAGSIMAKRVEMTKMPELVAFMHSMIG
                     LAAVFIAIAAVVEPQSLGIVAALGDAIPAGNRLELFLGAAIGAITFSGSVIAFGKLSG
                     KYKFRLFQGAPVQFKGQHWVNLAIGLAILGLGLYFTFTGNLTAFAILLALAFVIGVLI
                     IIPIGGADMPVVVSMLNSYSGWAAAGIGFSLNNSMLIIAGSLVGSSGAILSYIMCKAM
                     NRSFFNVILGGFGGAVEAAGPAGAKEARPVKSGSSDDAAFLLTNADTVIIVPGYGLAV
                     ARAQHALMELAEKLTHRGVTVKYAIHPVAGRMPGHMNVLLAEAEVPYEQVFEMDDINS
                     EFGQADVVLVLGANDVVNPAAKNDPKSPIAGMPILEAYKAKTVIVNKRSMASGYAGLD
                     NELFYLDKTMMVFGDAKKVIEDMVKAVD"
BASE COUNT         1858 a         3139 c         3475 g         2287 t
ORIGIN      
        1 tgttcgcccc agcgggaaat ttcgtcctgc gcgagtctgc caagccactg gtgctgatca
       61 ctgcgggtgt cggcatcacc cctgcgctga gcatgttgga agcggcgcgc gacacgggcc
      121 gcgatatcca ctttatccac tgcgcccgcc acgccggcgt acacgccttc cgtgactggg
      181 tggaggcgca gggcgataag cgtccgcagc tgcgccatta cgtgtgttac agcgaaccgc
      241 gtgaaggcga tgcgggcgat gctgaggggt ttctcagcct ggaacagttg gctgagtggc
      301 tgcccgagca gcgcgatctg gatgcctact tcctcggccc caagccgttt atggcgcagg
      361 tcaaacggca tctgcacagc ctcggcgtac cgacggcgca aagccattac gagttctttg
      421 gtccggcaag cgcgctggac agttaataac ccggtctcgg aggtgattat gttggcgtat
      481 ctatcctgcc catcgcgggt actgcaactg gcgaaccgca tgctgctggc cggtgtgctg
      541 ctgttgctca gcggcttgct gggcgcctat ggcctggagg cgcagctgag catgggcagc
      601 ctggtcacgg cgcactcgct gacgatcatc ggcccaggcc tgctcaagct cggttatgta
      661 ctgcgcctgg ctgcgcagca gcatctgcgc aagcagcagg agccttgctg tgcagttgcg
      721 tgatcgccag caggagctgg ccattgcgcc attgtggcgg ctgggctttc gcccgttctt
      781 tctcggtggc gcgctgtttg ccgtgttggc gattggtctg tgggtggcag tgctactggg
      841 gtatctgccg ggttggcagc cggtgggcgg cagcctggcc tggcaccggc atgagctgcc
      901 gttcggcttt gccgtggcga ttattgccgg ctttctgctg accgccgtgc agaactggac
      961 gggacggccg ggcctgagcg gttggccgct ggccgcgctg ttcggtctct ggctattggc
     1021 gcgcctggcc tggctgctcg gcgcgccgtt gccactcttg ctggcgctgc aactgccgtt
     1081 tctaccgctg ctggcgctgt tgcttgggcg gcagctgtgg caggtacgcc agtggcgcaa
     1141 ctacccgctg gtggttgtgg tgttgctgat ggcgggttgc gagtggctgc tgctgtatgg
     1201 cctggcgctg ggtgatgacg ccctgcagcg gcgcggtgcc ttggccggtt tatggctgat
     1261 tgctgcgctg gtgtgcatta tcggcgggcg ggtgattccg ttctttaccc aacgcggcct
     1321 caatcgagcg gagcctgctg caccgcggcc gcgcctggat ctgctggtgc tggtgttgtc
     1381 gctcagtctg gcgctgttgt ttgccagtgg cctggctttg cagccgtctg tctggatgct
     1441 gccgctgttg ctggttttgg tgatgctgca tggcctgcgc ttgcagcact ggtatgacgg
     1501 cgggatctgg cgggtgccac tgctgtggtc gctgcacctg gcctacgctt ggttgccgct
     1561 ggcgttactg gccctggtgg cctggcatgc cggttggctg agcaacgcca gcttggccta
     1621 tcacgccctg gcattgggca cggtgggcgg tgcgattttg gcgatgctgg cgcgggtcag
     1681 cctcggccat accggtcggc cgttgcagcc gccggcgctg atgggctggg cgtttgccag
     1741 tctgcagctg gcagttgtgg cgcgggtgct gctggtgccg tttgcgcccg gcacagggct
     1801 ggggctatcc agtctgctgt ggatcgtggc ctttgccctg tttttacggc attacgcgtc
     1861 gatgttctgg caggcgcggg tggatggtca gccggggtag gtggtgcatc aagctgcgcg
     1921 ctcggagttg gtagaggtag ccagggccgg ctgtgaggtc gtggtgcgcg ctgcgcaccc
     1981 tacgggatcg gctagtgagc tatcgggaga aggtcttcag tagaaacgct agctgatccg
     2041 ctaggctctt ttctcgattc tccccctgat aaatgtcgaa atgccccatg tcgtaacggc
     2101 gcacttctgc gtgcttcatt ttcttcgccg ctttctctgc tgctgccact ggagcaacgg
     2161 tgtccttttc gcaaatctgg atcagcgccg ggcaagtgac cttgtgcgct tcagttaccg
     2221 ggcggaaggc cagcaggctg ctggagacgc gcgccgccac cgtgttaggc acaccttcgg
     2281 gtatcagggc gagatagccg tcatagcaat ctgctgcagt cattgctgcc actgcgccgg
     2341 gccggccggc ggaagcaatg tagtgcggtg acatgccaag cagggcgcgg gttatatcca
     2401 gcgcgccgtg ggcgctgagt ttcagtacat gggctacgcc ggcatagcgg gtcactgcga
     2461 gtaccgaagc caggccatcc atcatggggc attgagcaat gatggcctgc acattgccgt
     2521 cacgcgcagc ggcgactgtg acgaggccgc caccgaacga ggtgccccac agggcgattg
     2581 cgtcgggctt gatgccatta agcttgcgca cgaaggcgat agcgcagagc cagtcttcca
     2641 cttcccgcga cggcaccagc agttgacgcg gttggccgtc gctggcaccg aagtggcggt
     2701 agtcgaacat aaagacggca taaccggcgt tggcgaaggc ttcggcaaag ggcgcgatgc
     2761 cacattcgcg ggtggcggca aagccgtgac ccatgatcac gcagggcacc ggacctatgg
     2821 catccgccgg catatacagg tagcccttgc aggtaatgcc gtggctggtg aaggcaagat
     2881 cagtgcgcgt gaacatgctg tgttccttgc gggtgtagct gttgcgcggt cggtcgaccg
     2941 tttccaggag tttccaggta tcgacggctg cgccgagcac caggaaatac gggttgagaa
     3001 tggcctgctt gttttactgc agcagcccga agggcgtatt ggccacggtg ccagcggcaa
     3061 tgcgcgctac ggcatcttct gcatcggctc gagggtggca ggatcagtgg ctgtgggcgg
     3121 gggcgtcatc gctttcttta cccggcgggc gcgggcttaa tcggctcacg cctgttttgc
     3181 cccgcctcac ctggattgtt caacggcgta tcaggcggcg gatatagctg cagatcggca
     3241 ccctgggtgg tcgattaaaa aacgaggggc tcagagcacc ccagcctctc gcagcgagtt
     3301 gatctgttcc gcgctcaggc ccaggtgccg ggtcagtacc tgttcggtgt gttcgcccag
     3361 cagcggtggc gcgttgcggt attccaccgg ggtttccgat aggcgcagtg ggctggcgac
     3421 ttgtggcgta gtgctgccca ggctgttggg cagatcgagg cgcaggccgc gcgcttgtac
     3481 ctgggggtcg gcaaatacct gggccaggtc gttgatcggc ccgcagggca cgccggcggc
     3541 ttccagcagc gttacccatt cggcggtggt tttgaatacc gtggcctggc gcagcagtgg
     3601 gatcaactcg gcgcggtggg tgacgcgggc cttgttgctg gcaaaacgcg ggtcatcggc
     3661 caaagccgca cagccagcaa cctcgcaaag tttgcggaac tgcccgtcgt tgcccacggc
     3721 gataatgaag ttgccgtcgg ccgaggggaa atcctggtag ggcacgatat tcgggtgagc
     3781 gttacccaag cgtttgggcg cgaccccggt attcaggtag ttcatcgcct gattggccag
     3841 gcaggccacc tgcacgtcga gcagcgccac gtcgatatgt tggccgaggc cggattgctc
     3901 acgctgattg agtgccgcca gcacgccgac cgtggcatac aggccggtaa ggatatcggt
     3961 gagggcgacg ccgaccttga tggggccggc gccctcctca ccgtcgggtt tgccggtcag
     4021 gctcatcaac ccgccgaggc cctggatcat aaagtcgtag cccgcgcgct tggagtaagg
     4081 cccgttttgc ccgaagccgg tgatggagca gtagatcagc cgtgggttga tggctttaag
     4141 cgacgcgtag tcaaggccat aagtcgccag accgccgacc ttgaagttct ccagcagtac
     4201 gtcgcagttc tgcaccagct cgcgcaccag gcgctggcct tccggctggg tgaaatccag
     4261 ggtcaccgac tgtttgttgc ggttggcgct ctggaagtag gccgcctccc gtgagtcatt
     4321 gccctcggcg tctttcacat agggcgggcc ccagtggcgg gtgtcgtcgc ctgagccggg
     4381 gcgttcgacc ttgattacct cggcacccag atcgccgaga atctgcccgg cccaaggccc
     4441 tgcgagtacg cgggacagat cgagaacgcg gatatgcgac agtgcgccgg gcatggtgag
     4501 cctcgtaaaa aatgaagggg gtgaatcggt ttaagatcgt cgcgaacgca gcattaccaa
     4561 gcgcagcaga tcttcaaccg attctagaag aacgcctgca ggccggtctg agctctgccg
     4621 aggatcagcg cgtggacgtc atgggtgcct tcataggtgt tcaccacttc caggttgacc
     4681 aggtggcggg ccacgccgta ctcgtcactg atgccgttgc cgccgagcat gtcgcgggcc
     4741 atacgggcga tatccaggga cttgccgcag ctgttgcgtt tcatcatcga ggtgatttcc
     4801 actgcagcgg tgccttcgtc cttcatccgg ccgaggcgca ggcagccttg cagggccatg
     4861 gtgatttcgg tctgcatatc ggccagcttt ttctggatca gctgggtctg cgccagcggg
     4921 cggccgaact gtttgcggtc gagggtgtac tggcgcgcgg tgtgccagca gaactcggct
     4981 gcacccagcg cgccccagct gatgccataa cgggcggagt tgaggcaggt gaacgggcct
     5041 ttcaggccgc gcacgtcggg gaaggcgttt tcttccgggc agaacacgtt gtccatgacg
     5101 atttcaccgg tgatcgacgc acgcaggccg accttgccgt gaatcgccgg ggtgctcagg
     5161 ccctgccagc ctttttccag cacaaagccg cgaatctcgc cggcatcgtc cttggcccag
     5221 accacaaata catcggcgat cgggctgttg gtgatccaca tcttcgcgcc ggtcaggcgg
     5281 tagccgccgt cgaccttctt cgcgcgggta atcattgagc ctgggtcgga gccgtgatcc
     5341 ggctcggtga ggccgaagca gccgatatat tcgccgctgg ccagtttggg caggtacttc
     5401 tgcttggtcg cttcgttgcc gaactcgaag atcggcacca tcaccagtga cgactgcacg
     5461 ctcatcatcg agcggtagcc ggagtcgatg cgctcgactt cgcgggcaat taggccgtag
     5521 cacacgtagt tcaggccgct gccgccgtac tcggcgggaa tggtggcgcc gagcaggccg
     5581 gtgtcgccca tctcgcggaa gattttcggg tcggtttgct cgtggcggaa ggcttcaagt
     5641 acgcgcgggg ccagcttgtc ggcggcgaac tgctgggcgc tgtcacgcac catgcgttct
     5701 tcttcggtca gttgctggtc gagcagcagc gggtcgatcc agttgaagct tgccttgcca
     5761 gccataagcg aaatcctcgg tcgaaatgcg tagagagtct tggtaggagc cagcttgctg
     5821 gcgatgcctt ttttcgccag caagaactgg gcgtccccct cgctcctaca gacgaaacag
     5881 tgggattgat cctaggcggc ttgtggcagg caggcaaacg agtattgcgc acgcagttgt
     5941 gcgaattact cacttcgaga tagctttagt cagcatattt aatagctcta agtgagggtg
     6001 acgcacagat gcgccgcaag atccccagca ccgccgccct gatcgctttc gagtccgctg
     6061 cgcggcacca gagttttacc aaggcggcgc aggaactgag cctgacccag ggcgccgtgt
     6121 gtcggcagat tgccgcgctg gaggccttcc ttaatatcga gctgttccgc cgttcgcgcc
     6181 gtggcgtggt gctcaccgag gccgggcagt cctacgcgcg gcgagtggcc ggtcagcttg
     6241 atgcggtgga gcgcgatacc ctggcggtga tgggccagca gggcgcgatg agtatcgaac
     6301 tggcggtggt gccgaccttc ggtacgcaat ggctgctgcc gcgcttgaag gactttcagc
     6361 ggttgcaccc ggagatcacg gtcaacctga ccaaccgcac gcgacccttc ctgtttgccg
     6421 ataccgagtt tgacgcggcg gtgtatttcg gcgatggcga ctggtctggt actcaggccc
     6481 acttgctgat gggcgaaaac cctatgccgg tatgcagccc ggcgttgctg gctgagcaga
     6541 cgcagctaag cgccgagcag attgccaacc tgccgctgtt gcagcagacc acgcggccct
     6601 atgcctggcg ccagtggttc agtgcccagg ggctgaacac ctcgcgggat atggccgggc
     6661 cgcgttatga gttgttctcc atgctggccc aggcggccat gcacgaaatg ggggtggcgc
     6721 tgattccgcc gtttctgatc cgccgcgagc tggatgaagg acgcttggtg attgctgcac
     6781 cgctgagcct gccgaccgac aaggcgtatt acctgatgat ccccgagcgc aagctggagt
     6841 cggcggcgct gttgctgctg cgcgactggc tggtgcgcga ggcccatgtc tatgccttgg
     6901 cccatggctt gaaatagaca acagcttgtc aggatgttgg cggaaaaagc cgtcaaacct
     6961 tcggcggtaa gttattggaa ataacgctac ctcgcgagac aggccggcta gagtgcgcgc
     7021 cggcgttata ttgaccccgt agtcagttat ctgaatggct acactttctt tgcgcatggc
     7081 tgacatcggg tgttgcattt tttgcacaca aacgctgcta cagcccaatg aaacagcgtt
     7141 ttatccggta aatgctggcg gcgccgttga tctgatggcg ctgtatttat ctcgggaatg
     7201 gataagcgct gttttacccc gctgaatgcg ctgcaggcct taccccaaaa gggctgccat
     7261 gaattagctg cctgtagggt catccagtga cttgtagtta tcacaatgtt ttacttcata
     7321 acgccttgaa ggctatggcc ttcacctgca aaatgccgcg ccccgccatt gtgcaactgc
     7381 agcctgagcg ggtttgtgct gatcctccct aatgccagcg tgccatccgc agtgcgccgg
     7441 ttcacaaaaa agatcacgca ggagaattga agtgcacatc ggtgttcctc tcgaaaccca
     7501 tgctggtgag acgcgcgttg ccgcaactcc cgaaaccatc aagaagctga tcggccaagg
     7561 ccatcaggtg actgtacaga ccggtgctgg cgttagcgca agcattccgg acagcgccta
     7621 tgaagctgtt ggcgcggcta tcggtaacga cgctgctgcc tttggtgccg atctggtgct
     7681 gaaagtggtc gccccgaccg acgctgaact ggcgcacatg aaagccggtg ccgtattggt
     7741 gggcatgctc aacccgttca gcaacgagac catcgcgcgc atcaatgctc gcggcgtgac
     7801 cgccttcgcc ctggaagctg ctccgcgcac ttcccgcgcg cagagcctgg acgtgctcag
     7861 ctcgcaagcc aacatcgccg gctacaaggc cgtgctgctg gctgcgcacc actacccgcg
     7921 ctttatgccg atgttgatga ctgctgccgg taccgttaag gccgcgcgca ttctgattct
     7981 cggcgccggt gttgctggtc tgcaggctat cgccacggcc aagcgcctgg gtgcggtgat
     8041 cgaggcctcg gacgtgcgtc cggcggtgaa ggagcagatc gaatcgctcg gcgccaagtt
     8101 cgtcgacgta ccgttcgaga ccgatgaaga acgcgaatgc gcccagggcg ttggcggcta
     8161 cgcgcggccg atgccggctt cgtggatgga gcgtcaggcc aaggcggtgc acgaacgcgc
     8221 caagcaggcc gatatcgtta tcaccaccgc gctgatccca ggccgcaagg cgccgactct
     8281 gctgcacgcc gccaccgtgg cggaaatgaa gcctggttcg gtgatcatcg acctggctgc
     8341 agcacaaggc ggcaactgcc cgctgaccgt cgctgagcag gtggtggtgc agcatggcgt
     8401 gaccatcgtc ggccacagca acctggcggc cctggtgcca gccgatgctt cggcgctgta
     8461 tgcacgcaat ctgctggact tcctcaagct ggttatcgac ggcgaaggca agttccacct
     8521 caaccttgaa gacgatatcg tcgccgcgtg cctgatgtgc cgtgacggca acgtcgtgcg
     8581 cactaacggc taaggggagc accgctcatg gacatgattt ctgacggcat ctacaacctg
     8641 atcatcttcg tgctggccat ttacgtgggc taccacgtgg tgtggaacgt gactccggcc
     8701 ctgcacaccc cgctgatggc ggtgaccaac gcaatttccg cgatcgttat cgtcggcgcc
     8761 atgttggctg ccgctctgac cgtgaccccg ctgggcaaga ccatgggcac cctggccgtg
     8821 gccctggctg cggttaacgt gttcggtggc ttcctggtaa cccggcgcat gctggaaatg
     8881 ttcaagaaaa aggcacctaa agctgctgct ggggctgcca ccaagggaga gaaccattga
     8941 tgagcatgaa tctgatcact gttctctacc tcgtcgcctc gatctgcttt atccaggcgc
     9001 tcaaaggcct gtcgcacccg actacttcgc gtcgcggcaa cctgttcggc atgatcggca
     9061 tgggactggc ggtgctgact accgttggcc tgatctacaa gctcggtgct gaaattgcca
     9121 cccagggcat tggctacgtg attgtcggcc tgctggtcgg cggtactgct ggctcgatca
     9181 tggccaagcg cgtggaaatg accaagatgc cagaactggt cgctttcatg cacagcatga
     9241 tcggcctggc tgcggtgttt atcgccattg ctgccgtggt tgagccgcag tcgctgggta
     9301 tcgttgccgc gttgggtgat gcgattccgg ccggtaaccg tctggagctg ttcctcggtg
     9361 cggccatcgg tgccatcacc ttctccggtt cggtgatcgc cttcggcaag ctctcgggca
     9421 agtacaagtt ccgcctgttc cagggcgcac cggtacagtt caagggccaa cactgggtca
     9481 acttggccat cggcctggcg attctcggtt tgggtctgta cttcaccttc accggtaacc
     9541 tgacggcgtt cgccatcctg ctggccctgg ccttcgtgat cggcgtgctg atcatcatcc
     9601 cgattggcgg cgcggacatg ccggtagtcg tgtcgatgct caacagctac tcgggctggg
     9661 cggcggccgg tatcggcttc tcgctgaaca actcgatgct gatcatcgcc ggttcgctgg
     9721 tgggttcgag cggtgcgatc ctctcgtaca tcatgtgcaa ggcaatgaac cgctcgttct
     9781 tcaacgtgat cctcggtggt ttcggcggtg cggttgaagc agccggcccg gctggcgcca
     9841 aggaagctcg tccggttaag tccggttcaa gcgacgacgc ggccttcctg ctgaccaacg
     9901 ccgacaccgt gatcatcgtt ccgggctacg gcctggcggt agcccgtgct cagcacgcgc
     9961 tgatggagct ggcggaaaag ctgacccacc gtggcgtgac cgtgaagtac gcgatccacc
    10021 cggttgccgg tcgtatgcct ggccatatga acgtgctgct ggccgaggcc gaagtgcctt
    10081 acgagcaagt gttcgagatg gacgacatca actctgagtt cggccaggcc gacgtggtgc
    10141 tggtgctcgg cgctaacgac gtggtcaacc cggcggcgaa gaacgatccg aagtcgccga
    10201 tcgccggcat gccgatcctc gaggcttata aagccaagac cgtgatcgtc aacaagcgct
    10261 cgatggccag cggctacgcc ggcctggaca acgaactgtt ctacctggac aagaccatga
    10321 tggttttcgg cgacgccaag aaagtcatcg aagacatggt taaagcggtc gactaatcct
    10381 cgctgcgacg ctgtagaaaa agcccggctc aatagccggg cttttttctg tccactcgat
    10441 cagaaaagtg tttttctcaa ggcaaaatct gaaaaacagg tcagctgtca cataagtgca
    10501 atttgaaact gtacctatat ggtttcactg aaatttagca ttaaccgcag gtctttcatc
    10561 tgctttagtg ccatttttag gctaaagcac ttatggccag tacagttgag tgcaaaacag
    10621 gtcaaacagt tacaaagcaa actatctatt tgtgctgcaa tttcattcag ctgtggctac
    10681 agcgaagtgg gggcaacggc aataattctc ccatcgcaac acaccactga cccgccacaa
    10741 gcggaaggat gaccaccat
//
LOCUS       DASRUM010000232         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_288518, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000232 DASRUM010000000
VERSION     DASRUM010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288518"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..135
                     /locus_tag="VFF22_15545"
     CDS             <1..135
                     /locus_tag="VFF22_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HZX18264.1"
                     /translation="ALPDTRIDGLSGSLSLNPAQRIERQLPWAEFRGGRVEHLPDSLN
                     "
     gene            135..506
                     /locus_tag="VFF22_15550"
     CDS             135..506
                     /locus_tag="VFF22_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016779518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HZX18265.1"
                     /translation="MDHGSTQSSGKAAEELARQHLLQHGLKLLAQNWSCRSGELDLVM
                     LDADTVVFVEVRYRRHSAWGGAVESVDARKREKLTKAALHFLQRESRWAKHPCRFDVI
                     AINAEGNSTPRLNWIQNAFDT"
     gene            537..1130
                     /locus_tag="VFF22_15555"
     CDS             537..1130
                     /locus_tag="VFF22_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HZX18266.1"
                     /translation="MEMQDRIRQLFLASIETKQQALEVLVPFIEQGSLAMVQALLSEG
                     KILTCGNGGSAGDAQHFSSELLNRFERERPSLPAIALTTDSSTITSIANDYSYNEIFS
                     KQIRALGQPGDVLLAISTSGNSANVIQAIQAAHDREMTVVALTGRDGGGMASLLLPED
                     VEIRVPSKITARIQEVHLLAIHCLCDLIDSQLFGSEE"
     gene            1127..1660
                     /locus_tag="VFF22_15560"
     CDS             1127..1660
                     /locus_tag="VFF22_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZX18267.1"
                     /translation="MTRSPLILAALALTLALAGCGNRSVGNKIDDQFIPSEVSSSVAR
                     AHIDLTSPTSRIVVTSYNGVVLLAGQTPRSDLKAMAEQAARKVNNVKKVHNELQVLKP
                     TSLLVRSNDSLLTSRIKTQMLADSSVPGTRIKVITENGIVYMLGVVSQAEATHATNLV
                     QSVSGVQKVVKLFEYTN"
     gene            complement(1730..2143)
                     /locus_tag="VFF22_15565"
     CDS             complement(1730..2143)
                     /locus_tag="VFF22_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HZX18268.1"
                     /translation="MKSSRPYLVRALYEWIVDNDCTPHLLVNAEFAGVQVPSGFASDG
                     QIVLNVSPTAVRHLHMDNEAVSFEGRFAGVPQSLYIPAAAVLAIYARENGQGMVFDAE
                     SPMLDGDEIEDPDEQSPPDDEPPRPSGRPSLKVVK"
BASE COUNT          489 a          744 c          599 g          380 t
ORIGIN      
        1 gcccttccgg atacccgcat cgatggcctg tccggcagcc tcagcctcaa cccggcacag
       61 cgcatcgagc gccagctgcc ttgggccgag ttccgcggcg gtcgcgtcga acacctgcca
      121 gacagcctca attgattgac cacggcagca cgcagagcag cggcaaggca gccgaagagc
      181 tggcccgtca gcatctgctg cagcacggcc tgaagctgct ggcgcagaac tggagctgcc
      241 gcagcggcga gcttgatctg gtcatgctcg acgccgatac agtagtattc gtcgaagtcc
      301 gctaccgccg acactccgct tggggcggcg ccgtggagag cgtcgacgcg cgcaagcgcg
      361 agaagctgac gaaagccgcc ctacactttt tgcagcggga atcgcgctgg gccaaacacc
      421 cctgccggtt cgacgtgatt gccataaacg ccgaaggcaa ctcgactccc cggctgaact
      481 ggattcaaaa cgcctttgac acctgaaccg gcgccacact gaaggttaaa tctccgatgg
      541 aaatgcaaga ccgtatccgc caactgttcc tggccagcat tgaaaccaag cagcaggctc
      601 tggaagtact ggttccgttc atcgagcaag gcagcctggc catggttcaa gccctgctca
      661 gcgagggcaa gatccttacc tgcggcaatg gcggctctgc tggcgacgcc cagcacttct
      721 cctccgagct gctcaaccgc ttcgagcgcg agcgcccgag cctgccggcc atcgccctga
      781 ctaccgacag ctcgaccatc acctcgattg ccaatgacta cagctacaac gagatcttct
      841 ccaagcagat ccgcgccctc ggccaacccg gcgatgtgct gctggcgatt tccaccagcg
      901 gtaactcggc caacgtgatc caggccatcc aggccgccca tgaccgcgaa atgaccgtgg
      961 ttgccctcac cggccgcgat ggcggcggca tggcctcact gctgctgccg gaagacgtgg
     1021 aaatccgcgt cccctcaaaa atcaccgcac gcattcagga agtccacctg ctggccatcc
     1081 actgcctgtg cgacctgatc gacagccaac tgtttgggag tgaagaatga cccgttcacc
     1141 actgatcctc gccgccctag cactgactct cgccttggcc ggctgcggta accgcagcgt
     1201 cggcaacaag atcgacgacc agttcatccc gtccgaggtc agctccagcg tggctcgcgc
     1261 gcatatcgac ctgaccagcc caacctcgcg aatcgtggtt accagctaca acggcgtagt
     1321 gctactggca ggccagacgc cacgcagcga cctcaaggcc atggccgagc aggccgcgcg
     1381 caaggtcaac aacgtgaaga aggtgcacaa cgaactgcaa gtgctgaagc ccacgtcgct
     1441 gctggtgcgc agcaacgact cgctactaac cagccgcatc aagacccaga tgctcgctga
     1501 cagcagcgtg cccggcactc gcatcaaggt gatcaccgaa aacggtatcg tctatatgct
     1561 cggtgtggtc tcgcaggccg aagccacgca cgccaccaac ctggtgcaaa gcgtctccgg
     1621 cgtgcagaag gtggtgaagc tgttcgagta caccaactga tagcaccatc cagacaccgc
     1681 ctgcgtggcg gcaataaaaa aggcgatctt cggatcgcct tttttatgtt tacttgacca
     1741 ctttcagact gggcctgcca ctgggacgcg gcggctcatc atccggtggg ctttgctcat
     1801 ccggatcttc aatctcatca ccatcaagca tcggcgattc agcatcaaac accatgccct
     1861 ggccgttctc ccgcgcataa atcgccagca cggcagctgc ggggatgtac aggctttgcg
     1921 gcaccccagc aaaacgccct tcaaagctca ccgcctcgtt atccatatgc agatgacgca
     1981 cggcagtagg cgagacattc agcacgatct gcccatcact ggcaaaaccg gaaggcacct
     2041 gcacaccggc aaactcggca ttgaccagca gatgcggggt gcagtcgttg tccacgatcc
     2101 actcatagag ggcacgaacc agataaggac gactcgactt catcactacc tcctcgggtt
     2161 aacgcatatc acgttcgaca ggcgacagac tggcctggaa cgcttcacga gc
//
LOCUS       DASRUM010000233        12877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1950361, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000233 DASRUM010000000
VERSION     DASRUM010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 12877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12877
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950361"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFF22_15570"
     CDS             complement(<1..594)
                     /locus_tag="VFF22_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003112756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX18269.1"
                     /translation="MPRKSWRDAIAAYSSPSTLVLLLLGFAAGLPYMLVFSTLSVWLR
                     EAGVARDTIGFASLIGLAYAFKWVWSPLLDQWRLPLLGKLGRRRSWLVLSQILIAIGL
                     AGMALCDLQTHLTWLISLAVLVAFASATQDIAVDAYRLEIVDDTRQAALAASYMAGYR
                     VAALLATAGALYFAEGFGSTALLYQHGAWAGTYLMFAL"
     gene            complement(638..1027)
                     /locus_tag="VFF22_15575"
     CDS             complement(638..1027)
                     /locus_tag="VFF22_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HZX18270.1"
                     /translation="MGKPDSEPKQGSKHAAPGQPTASQVERRTALYAVLAQIPAGCVV
                     SYGQLAAYAGLGRAARWVGRTLSQLPDGSTLPWHRVIAASGRLSLPAGSLSGAEQRSR
                     LREEGVQIHNDRVDMPRYGWRPMEHSG"
     gene            1029..2027
                     /locus_tag="VFF22_15580"
     CDS             1029..2027
                     /locus_tag="VFF22_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HZX18271.1"
                     /translation="MLLRTLLCSLVFALAAPAWADTVWLNNGDRLSGEIVLLDGGKLA
                     LKTKYAGQVLIAWKDIDTLRSEKPLLMRRQGLDSEHSNQLEAAGKGMVRVVSGNTQTV
                     PLSSIKRLIPPRPVLQDRFWEGNLDAKLDMKRDDNDTDEWKFKGNTRMEHGRWRHVLA
                     GELERKVKNEAKTADNWQLEYDLDRFLTDHWFWRVGAEQDEDEFAFFTRQRSLGTGPG
                     YRFWDNELGRLDVIGQFNRLQLESTDGELALNTYSLSWDYKRLLWGTRLEFYSTAELQ
                     LPEIDEIDYVLDSEMGLRYRLTDWARLSLLYELDQARGLGQTSSERRYLLGVGVGW"
     gene            2137..3141
                     /locus_tag="VFF22_15585"
     CDS             2137..3141
                     /locus_tag="VFF22_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HZX18272.1"
                     /translation="MFSRTLLCLALSVAATSALADTVWLKNGDRLTGKIKFYDGGKLL
                     LETDYGGSIALDWAKIATLESDQELLIKENAIVGERAKSLQPAEPGKVILANGDAPKT
                     IELASIKQILKPKPLVEDLVWKGNIDAALDYKRAERDTDDYNVNLKTQARHGLWRHHG
                     EAEYHREFQDDVVTTDNWSTEYALDRFLTEKWFWQGRFEYKRDMVEELERQRTVGTGP
                     GYQFWDNELGAFSVTSLLNRTDYEYSNGESDNFYAASLKWDYNRYLIGKSVELFSDGE
                     VGKPLNNVADYTLDAAVGLRYKLTDWASLNMKAEKDLVSGSEGELDETRYSLGFGVGW
                     "
     gene            complement(3331..4089)
                     /locus_tag="VFF22_15590"
     CDS             complement(3331..4089)
                     /locus_tag="VFF22_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX18273.1"
                     /translation="MQLKDKVIIITGGCQGLGRAMAEYLADKGVKLALVDLNQEKLDE
                     AVAACKAQGVEARAYLCNVANEEQVTQTVAQIAEDFGAINGLVNNAGILRDGLTIKVK
                     DGEITKLSLAQWQSVIDVNLTGVFLCTREVAAKMIELKNEGAIINISSISRAGNIGQA
                     NYSAAKAGVAADTVVWAKELARYGIRVAGVAPGFIETEMVASMKPEALERMTSVIPLR
                     RMGKPAEIAHSVAYILENDYYTGRILELDGGLRL"
     gene            4183..4914
                     /locus_tag="VFF22_15595"
     CDS             4183..4914
                     /locus_tag="VFF22_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HugZ family protein"
                     /protein_id="HZX18274.1"
                     /translation="MSVKAGKHARELLLKEYRGVLSTHSKAMPGFPFGSVVPYCLDAD
                     GYPLILISRIAQHTHNLKQDGKCSLLVGERGAEDVQAVGRLTLLAQARQLDDEAQIAA
                     AALRYYRYFPDSRGYHQAHDFDFWRLEPVRWRYIGGFGAIHWLDQVTLANPFAGDSEI
                     SMIEHMNSDHASAIAHYVELAGLPSHAPAELVGIDSEGFHLRIGQGLYWLAFPTSCNS
                     PLEVRQALVALARAEVWPTGGQASA"
     gene            4978..5427
                     /locus_tag="VFF22_15600"
     CDS             4978..5427
                     /locus_tag="VFF22_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HZX18275.1"
                     /translation="MRAFLFLFLLFPIIELALLIQVGSAIGVLPTLLLVIGTAILGSV
                     LLRVAGVATAWRAREKLARGELPEQEMLEGLLIAVGGGLLLLPGFISDIFGVLCLIPF
                     TRRMLVNKVRLRAAEQAMRQRAFFDDQAARSGQARPNVLEGEYERRD"
     gene            5665..5958
                     /locus_tag="VFF22_15605"
     CDS             5665..5958
                     /locus_tag="VFF22_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020799908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZX18276.1"
                     /translation="MKLRPLHDRVVIRRSEEEKKTAGGIVLPGSAAEKANSGEILAVG
                     TGRVLDNGEVRALAVKVGDKVVFGPYSGSNTVKVDGEDLLVMSESEILAVVEG"
     gene            6018..7661
                     /gene="groL"
                     /locus_tag="VFF22_15610"
     CDS             6018..7661
                     /gene="groL"
                     /locus_tag="VFF22_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253075.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZX18277.1"
                     /translation="MAAKEVKFGDSARKKMLTGVNVLADAVKATLGPKGRNVIIEKSY
                     GAPTITKDGVSVAKEIELKDRFENMGAQLVKDVASRANDDAGDGTTTATVLAQAIVNE
                     GLKAVAAGMNPMDLKRGIDKATIAIVAELKKLSKPCADTKAIAQVGSISANSDTSIGE
                     IIAEAMEKVGKEGVITVEEGSGLENELSVVEGMQFDRGYLSPYFINKPDTMVAELDGP
                     LILLVDKKISNIRELLPVLEAVAKSGRPLLIVAEDVEGEALATLVVNNMRGIVKVAAV
                     KAPGFGDRRKAMLQDIAVLTGGTVISEEIGLSLESATLEHLGNAKRVILSKENTTIID
                     GAGQQTDIESRVAQIRKQVEDTSSDYDKEKLQERLAKLAGGVAVIKVGAGTEVEMKEK
                     KARVDDALHATRAAVEEGVVPGGGVALVRALQAISELKGDNADQDVGIALLRRAVEAP
                     LRQIVSNAGGEPSVVVDKVKQGSGNYGFNAATDTYGDMIEMGILDPAKVTRSALQAAA
                     SIGSLMITTEAMIAEVVEDKPAGGGMPDMGGMGGMGGMM"
     gene            8255..8479
                     /locus_tag="VFF22_15615"
     CDS             8255..8479
                     /locus_tag="VFF22_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016986677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18278.1"
                     /translation="MKLEIARGLFLVTALSVASLAAAAWQEASPQVITLNERGYCPLP
                     PQARTAEAIKPDQDLLLFMFSLSQGARSQG"
     gene            complement(8539..11586)
                     /locus_tag="VFF22_15620"
     CDS             complement(8539..11586)
                     /locus_tag="VFF22_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HZX18279.1"
                     /translation="MAFTDPFIRRPVLATVVSLLIILLGFQAFSKLTIRQYPQMENAL
                     ITVTTAYPGANAETIQGYITQPLQQSLASADGIDYMTSVSRQNVSIISIYARIGANSD
                     RLFTELLAKANEVKNQLPQDAEDPVLSKEAADSTALMYISFFSDELSNPQITDYLSRV
                     IQPKLATLPGMAEAEILGNQVFAMRLWLDPVKMAAYGVTASDVNDAVRKYNFLAAAGE
                     VKGEYVVTSINAETDLKSAEAFAAIPLKTVGDSRVLIRDVARVEMGAESYDSISSFDG
                     TPSVYIAIKGTPGANPLDVIKEVRKVLPDLEAQLPPNLKVSIAYDATLFIQASIDEVV
                     KTLFEAVLIVIVVVFLFLGAFRSVLIPVITIPLSMIGVMFFMQLMGYSINLLTLLAMV
                     LAIGLVVDDAIVVVENIHRHIEEGKTPFDAAIEGAREIAVPVVSMTITLAAVYAPIGF
                     LEGLTGALFKEFALTLAGAVIISGIVALTLSPMMCAKLLRHEENPSGLAHKLDQIFDS
                     LKQRYQRALHGTLDTRPVVLVFAVIVMCLIPVLLKFTKSELAPEEDQGIIFMMASAPQ
                     PTNLEYLNKYTDEFVTIFKEFPEYYSSFQINGFNGVQSGIGGFLMTPWNERERTQMEI
                     LPEVQARLSQIAGLQIFGFNLPSLPGTGEGLPFQFVINTPNDYQSLLQVTERVKQRAV
                     ESGKFAFLDVDLAFDKPEVVVEIDREKAAQMGVSMEDLGLTLASLLGEGEINRFTIDG
                     RSYKVIAQVERAYRDNPGWLSSYYVKSESGAMLALSTLVKVSDRARPTQLNQFQQLNS
                     AIIQGVPIVSMGEAIETVTQIMQEEAPAGYAYDFAGASRQLVQEGSALYVTFGLALAL
                     IFLVLAAQFESFRDPLVIMVTVPLSICGALIPLFLGLSSMNIYTQVGLVTLIGLITKH
                     GILIVEFANQLRREKGLSRREAVEEAAAIRLRPVLMTTAAMVFGMVPLILATGAGAVS
                     RFDIGLVIATGMSIGTLFTLFVLPCVYSLLAQPDAPPTTAKAH"
     gene            complement(11614..12750)
                     /locus_tag="VFF22_15625"
     CDS             complement(11614..12750)
                     /locus_tag="VFF22_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZX18280.1"
                     /translation="MLLRRMLIMLGAVLVVVLALAAYKGFSIYQQVQMFSAPQPAISV
                     DAATAEEHPWQGRLPAIGTLKAFQGVDLTVEVGGTVQQVLFRSGEQVTLQQPLIQMDS
                     DVEQASLGTAQAELGLARVEYERGRSLVNRQSISKSEFDRLSASLQKANASVAQLQAQ
                     LAKKRILAPFAGTIGIRQIDVGDYLSSGTTIATLQDLSKLYVDFFLPEQAVPKLAVGE
                     RVRFSVAAYPNEVFEGEIAAINPKVEDATRNVQVRAMLVNPDNKLLPGMFANLEVLLP
                     GEQTLIVVPETAITYTLYGNSVYVIGEKKAEDGQVVKDDKDQAELVVERRFVETGERR
                     EGQVVILKGLQAGEQVVSAGQLKLDNGAHVSIANANVEAKPNAE"
BASE COUNT         2576 a         3713 c         3951 g         2637 t
ORIGIN      
        1 cagcgcaaac atcaggtagg tgccggccca ggcgccgtgc tgatagagca gggccgtaga
       61 gccaaagcct tcggcgaaat acagcgcacc ggcggtcgcc agcagcgcgg cgacgcggta
      121 gccggccata tagctggcgg ccagcgctgc ctggcgggtg tcgtcgacga tttccaggcg
      181 gtaggcatcg acggcgatgt cctgggtggc cgaagcgaag gccaccagca cggccaggga
      241 aatcagccag gtcagatggg tttgcaggtc acacagggcc atgccggcca ggccgatggc
      301 gatcaggatc tgcgacagca ccagccagga acgtcggcgg cccagcttgc caagcagtgg
      361 caggcgccat tggtcgagca gtggcgacca gacccatttg aaggcgtatg cgaggccgat
      421 caggctggca aaaccaatgg tgtcgcgggc cacgccggct tcgcgcaacc agacagaaag
      481 ggtggagaac accaacatat aaggcaggcc agcggcgaac cctagaagga gcagcaccaa
      541 tgtcgagggg ctggagtagg cagcaatggc atcacgccag cttttacggg gcattgaagg
      601 ggtatcagcc tgagtttgac ctaataaagg gcgcactcta accgctgtgc tccattgggc
      661 gccagccata gcgcggcata tccacccgat cgttatggat ttgcacgcct tcctcgcgta
      721 gacggctgcg ttgctccgcg ccagagaggc tgccggcagg caaactgagg cgtccgctgg
      781 cggcaattac ccggtgccag ggcagtgtgc tgccgtctgg cagttggctt agggtgcgcc
      841 cgacccagcg tgcggcgcgg cctaaacctg cgtatgcggc caattgcccg taactcacca
      901 cacagccggc aggtatctgc gctagcaccg cataaagtgc cgtgcggcgt tcgacctggc
      961 ttgctgtcgg ctggcctggc gcggcatgct tcgatccctg ttttggctcg ctatccggct
     1021 ttcccatcat gctgctacgt actctgttgt gttctctggt ttttgctctg gccgcgcctg
     1081 cgtgggcgga cacggtgtgg ttgaacaatg gcgaccgttt gagcggcgag atagttctgc
     1141 tcgatggcgg caaactggcc ttgaaaacca agtacgccgg ccaggtgctg attgcctgga
     1201 aggatatcga caccctgcgc tcggagaaac ccttgctgat gcgccggcaa ggcctcgaca
     1261 gcgagcacag caaccaactg gaagctgctg gcaagggcat ggtacgggtg gtcagtggca
     1321 acacgcagac cgtgccgcta tccagcatca agcgcctgat cccgccgcgc ccggtgctgc
     1381 aagaccgctt ctgggaaggc aacctagacg ccaagctgga tatgaagcgc gatgacaacg
     1441 ataccgatga gtggaagttc aaaggtaata cgcgcatgga gcacgggcgc tggcgtcacg
     1501 tgttggccgg cgagctggag cgcaaggtca agaatgaggc gaagaccgcc gacaactggc
     1561 agctggaata tgacctcgac cgctttctca cggaccactg gttctggcgc gttggtgccg
     1621 agcaggatga ggatgagttc gcctttttta ctcgccaacg ctcgctgggg accgggccgg
     1681 gctatcgctt ctgggataac gaactgggcc ggctggatgt gatcggccag ttcaatcgtc
     1741 tgcaactgga atccactgat ggagaactgg cgctcaatac ctactcgctg tcctgggatt
     1801 acaagcgcct gctctggggc acccgcctgg agttttacag cacagccgaa ctgcagctgc
     1861 ctgagataga tgaaattgac tacgtgctcg acagcgaaat gggcctgcgt tatcgcctga
     1921 cggattgggc gcggttgtcg ctgttgtatg agctggacca ggcgcgtggg ctcgggcaaa
     1981 ccagcagtga acggcgctat ttgttgggtg ttggcgtcgg ttggtaggtc aaataggctg
     2041 aactcgtggt gattctgtcg gtcagtgctt gctctggcgt cgtgcttacg gataatgcgc
     2101 gaccttccgc gaactcgaag tagacaaacc attcctatgt tttccagaac cctgctgtgc
     2161 ctggcccttt cagtggctgc cacctccgcg ctggcggata ccgtctggct gaagaacggt
     2221 gaccgcctga ccggcaagat caagttctac gatggcggca agctgctgct ggaaaccgat
     2281 tacggcggct caatcgccct ggactgggca aaaatcgcca ccctggaaag tgatcaggag
     2341 ctgctgatca aggaaaatgc catcgtcggt gagcgcgcca agtccctgca gccggctgag
     2401 ccgggcaagg tgattctggc caacggcgat gcccccaaga ccatcgaact ggccagtatc
     2461 aagcagatcc tcaaacccaa gcccttggtt gaggatttgg tctggaaggg caatatcgat
     2521 gcggcattgg actacaagcg tgccgagcgt gacaccgatg attacaacgt caacctcaag
     2581 acccaggccc gccacggcct ttggcgacat catggcgaag ccgagtacca ccgcgagttc
     2641 caggacgacg tggtgaccac cgacaactgg agcaccgaat acgccctcga ccgcttcctg
     2701 accgagaagt ggttctggca ggggcgtttc gagtacaaac gcgacatggt cgaagagctt
     2761 gagcgccagc gcaccgtggg taccggccct ggttaccagt tctgggacaa cgagctgggt
     2821 gctttctctg tgaccagcct gcttaaccgc actgattacg aatacagcaa tggtgagagc
     2881 gacaacttct acgccgcgag cctgaagtgg gattacaacc gctacctgat cggcaagagt
     2941 gtcgagctgt tcagtgatgg cgaagtgggt aagccattga acaacgttgc ggattacacc
     3001 ctggatgccg ctgtgggcct gcgttacaag ctgaccgact gggcctcgct gaatatgaag
     3061 gccgagaagg acctggtcag cggctctgaa ggcgagttgg atgaaacccg ttacagcctg
     3121 ggttttggcg tcggttggta acgcttaacc gcggttcaaa tgaaaaagcc ccgcatttgc
     3181 ggggcttttt gttttcagca ttctagatct agcgagctag agtcagccta ggcaaaagca
     3241 ctcgcgggcg cggagtttac gagctgtaaa tgagcaagcc cgcgagtgct tttaacgacg
     3301 gatggccgac gcgcagcaga tcgatgcgaa ttacaaccgc agaccgccat ccagctccag
     3361 gatgcgaccg gtgtagtagt cgttttccag gatataggcc actgagtgag cgatctcggc
     3421 tggtttgccc atgcggcgca gtgggatgac cgaggtcatg cgctccagcg cttccggttt
     3481 catgctggca accatttcgg tttcgataaa gcccggtgct acgcctgcga cacggatgcc
     3541 gtaacgagcc agttccttgg cccagaccac ggtgtcggcg gctacgccgg ccttggcggc
     3601 ggagtagttg gcctggccga tattgccggc gcgggagatc gaagagatgt tgatgatcgc
     3661 gccttcgttc ttcagctcga tcatcttcgc cgccacttcg cgggtgcaga ggaatacgcc
     3721 ggtgaggttg acgtcgatta ccgactgcca ctgggccagg ctcagcttgg tgatttcgcc
     3781 gtccttgact ttgatggtca ggccgtcacg caggatgccg gcgttgttga ccaggccgtt
     3841 gatcgcgccg aagtcttcgg caatctgcgc cacggtctgg gtcacttgct cttcattggc
     3901 gacgttgcac aggtaagcgc gcgcttccac gccctgggcc ttgcaagcgg ccacggcttc
     3961 gtcgagtttt tcctggttca ggtcgaccag cgccagtttg acgcctttgt ctgccaggta
     4021 ttcagccatg gcgcggccca ggccctgaca gccgccggta atgatgatga ccttgtcttt
     4081 aagttgcatc gcgagtcccc tcaaatggtg tcgtgtggcc ttgccggcgg ttaatctagg
     4141 cgcctcccgt tctgtgacgg attttatgca aggagtcata aggtgagcgt gaaagccggc
     4201 aagcatgccc gagaattgct gctcaaggaa taccgtggcg tgctcagcac ccattccaaa
     4261 gccatgccgg gtttcccttt cggatcagtg gtgccctatt gcttggatgc tgacggctat
     4321 ccgctgatcc tgatcagccg catcgcccag cacacccaca acctcaagca ggacggcaaa
     4381 tgctcgctgc tggtgggcga gcgcggggcg gaggatgtgc aggcggtcgg ccgtcttact
     4441 ctgctcgcgc aagcacgaca attggatgac gaggcgcaga ttgctgccgc ggcgctgcgt
     4501 tattaccgct atttccctga ctcgcgcggt tatcaccagg cccatgactt cgacttctgg
     4561 cgcctggaac cggtgcgctg gcgctatatc ggtgggtttg gtgcgattca ttggctggat
     4621 caggtaaccc tggccaaccc ctttgcgggc gacagtgaaa tcagcatgat cgagcatatg
     4681 aacagcgacc atgccagcgc catcgcccat tatgtcgaac tggcaggtct gcccagccat
     4741 gcgccggcgg aactggttgg tatcgacagc gaaggtttcc atctacgcat cggtcagggc
     4801 ctatattggc tggcgtttcc aacatcctgt aacagtccgt tggaggtgcg ccaagccttg
     4861 gtggcactgg cccgcgccga ggtctggccg accggtggtc aggcttcagc ttgaattcag
     4921 ctcgacaccc catacttcac acttactgga agctgatctt ccgttaagga atctttgatg
     4981 cgtgcttttc tgtttctgtt tttgctgttt ccgatcatcg agctggccct gctgattcag
     5041 gtgggcagtg ccatcggtgt gttgccgacc ctcttactgg tcatcggcac cgccatcctt
     5101 ggcagtgtgc tgctgcgtgt cgcaggtgtt gccaccgcct ggcgtgcgcg ggaaaagctc
     5161 gcccgaggtg agttgcccga gcaggaaatg ctcgaaggtc tgctgatcgc cgtgggtggt
     5221 ggtctgctgt tgctgcctgg ctttatcagc gacatcttcg gtgtgctctg cctgattccc
     5281 ttcacccgca ggatgctggt caacaaggtg cgcctgcgtg ccgccgaaca ggccatgcgt
     5341 caacgcgcct ttttcgacga ccaggccgcg cgctccgggc aagcccgccc taatgtgctg
     5401 gaaggcgaat atgaacgccg cgattgagcc tgagtgcaac ctgcaggcct cgttttggca
     5461 gcgttgatcg caaaaataat catccgctgc ccttgaaatc cccttgtacg cccttatgta
     5521 gcggtcaccg caaggttttc tgttgttaga cagaatcaga cttcagcgct gcgcctggcg
     5581 tagcgctacc ggcctcgccg gactttgcta acccgctggt gaatacacca gccgctgtaa
     5641 accacctatt gggagagatc gacaatgaag cttcgtcctc tgcatgaccg cgtcgtaatc
     5701 cgtcgcagcg aagaagaaaa gaaaaccgcc ggtggcatcg tgctgccggg ctccgctgcc
     5761 gagaaagcca acagcggtga aatcctcgct gtcggtaccg gccgcgtgct ggacaacggt
     5821 gaagtgcgtg ccctggccgt taaagtcggc gacaaagtgg tgtttggccc ttactccggc
     5881 agcaacactg tcaaagttga cggcgaagac ctgctggtaa tgagcgagag cgaaattctc
     5941 gctgtagttg aaggttgatt tcagcgcccc gtcgctacga attccgctct atttgaacga
     6001 atcaaggaat aaccatcatg gctgctaaag aagttaaatt cggcgattcc gcccgcaaga
     6061 aaatgctcac tggtgtcaac gttctggctg acgcagttaa agcgaccctc ggcccgaaag
     6121 gccgtaacgt gatcatcgag aagagctacg gcgctccgac catcaccaag gacggcgttt
     6181 ccgttgccaa agaaatcgag ctgaaagatc gcttcgagaa catgggcgcg cagcttgtta
     6241 aagacgttgc ttcccgtgcc aacgatgacg ctggcgacgg caccaccacc gccaccgttc
     6301 tggctcaagc catcgtcaac gaaggcctga aagccgtcgc tgcaggcatg aacccgatgg
     6361 atctcaagcg cggcatcgac aaagcgacca tcgccatcgt tgctgagctg aagaagctgt
     6421 ccaagccttg cgctgacacc aaggccatcg ctcaggtagg ttcgatctcc gccaactccg
     6481 acacctccat cggcgaaatc attgccgaag ccatggagaa agtcggtaaa gaaggcgtga
     6541 tcaccgttga agaaggctcg ggcctggaaa acgaactgtc ggttgttgaa ggcatgcagt
     6601 tcgaccgtgg ctacctgtcc ccgtacttca tcaacaagcc ggacaccatg gttgccgagc
     6661 tcgacggccc gctgatcctg ctggtcgaca agaagatctc caacatccgc gaactgctgc
     6721 cagttctcga agccgtggcc aaatccggcc gtccgctgct gatcgtggcc gaagacgttg
     6781 aaggcgaagc cctggcgact ctggttgtga acaacatgcg tggcatcgtc aaagtcgctg
     6841 ccgttaaggc acctggcttc ggcgaccgtc gcaaggccat gctgcaggac atcgccgttc
     6901 tgactggcgg taccgttatc tccgaagaga tcggtctgag cctggaaagt gccaccctgg
     6961 agcacctggg taacgccaag cgcgtgatcc tgagcaagga aaacaccacc atcatcgacg
     7021 gtgctggtca gcagactgat atcgaatcgc gcgttgcgca gatccgtaag caggttgaag
     7081 acacctcgtc cgactacgac aaagagaagc tgcaagagcg tctggccaaa ctggctggcg
     7141 gtgttgcggt gatcaaagtc ggtgccggca ccgaagttga aatgaaagag aagaaagccc
     7201 gcgttgacga cgccctgcac gctacccgcg cagccgttga agaaggcgtg gtacctggcg
     7261 gtggcgtggc tctggttcgc gctctgcagg ccatcagcga gctgaaaggc gacaacgccg
     7321 atcaggacgt gggtatcgct ctgctgcgcc gtgctgttga agcaccgctg cgtcagatcg
     7381 tttccaacgc tggcggcgag ccaagcgttg tggtcgacaa ggtcaagcag ggttcgggca
     7441 actacggctt caacgccgct accgacacct acggcgacat gatcgagatg ggtattctcg
     7501 atccggccaa ggtcacccgc tcggcgctgc aagctgcagc ttcgatcggc agcctaatga
     7561 tcaccaccga agcgatgatc gccgaagtgg ttgaagacaa gccggccggt ggcggtatgc
     7621 cggacatggg cggcatgggt ggcatgggcg gcatgatgta agccagcccg gctcccgcag
     7681 gccatcgcca gatggcctgc acgttgtaaa gaaaaccccg tgcctgtcac ggggttttca
     7741 tttttctcgg cgataaacag tcctcgcggc aaagcggtac gccaattgct ttgtgtaatg
     7801 cgcgctcccg gactggagcc cgtcaatctg cgctgtgctg ccggtcagca tcgccgcttc
     7861 ttgttttgac ctcacctgga tgtgctctac cacgccgctt ttgacgtgtg gctggagatt
     7921 gtgccgttta cggttttgcc tgcggttgtt ttaaagccgc tggttctatg acgcaccacg
     7981 ctgatgcaat gggcaattaa gtgactttgg gttgttaccg ccaagtgcag tgataggccc
     8041 ccagtccttg gtttaccgaa gtgttgaacc attggcgcgc atgtggctcg aaggatggcc
     8101 tgctaaacgg ccagctgtgg atcaggatga tctgaatgaa gttgccggca gagtccggca
     8161 gtaacgaccg ctggtcgcaa aacgcgacat tgcggttatc agtgatctag ggtctacaaa
     8221 cagcgtttgg cagcgctatc aagaggttgc agtgatgaag ctagaaatcg cacgaggttt
     8281 gttcctggtg accgcgctca gcgtggcctc gctcgcggcg gcggcctggc aagaagccag
     8341 cccccaggtc atcaccctta acgagcgtgg ctactgtcca ctgcccccgc aggcgcgaac
     8401 ggccgaggcg atcaagcctg atcaggacct gctgctgttt atgttcagcc tgtcccaagg
     8461 tgctcgatcg caaggttgag cctgctgacc tagcaattcg atagacagaa aaaagccctg
     8521 cattgcaggg ctttttggtt agtgggcttt ggccgttgtc ggtggtgcat cgggttgcgc
     8581 cagcaggctg tagacgcacg gcaggacgaa cagagtaaac agcgtaccga tcgacatacc
     8641 ggtggcgatc accaggccga tgtcgaagcg gctgaccgca ccggcgccgg ttgccagaat
     8701 caacggcacc atgccgaaga ccatcgcagc ggtggtcatc agcaccggac gcaggcgaat
     8761 cgctgccgct tcttcgacgg cctcacgacg cgacaggcct ttctcgcggc gcagctggtt
     8821 ggcgaactcg acgatcagga tgccgtgctt ggtgatcagg ccgatcagcg ttaccaagcc
     8881 cacctgggtg tagatgttca tgctcgacag gccgaggaac agcggaatca gcgcgccgca
     8941 gatcgacagc ggcaccgtga ccatgatcac cagcgggtcg cggaagcttt cgaactgtgc
     9001 cgccagtacc aggaagatca gcgccagcgc caggccgaag gtcacgtaca gcgcgctgcc
     9061 ttcctgtacc aactggcgtg atgcgccggc gaagtcgtag gcgtaaccgg ccggggcttc
     9121 ttcctgcatg atctgcgtca ccgtctcgat ggcctcaccc atgctgacga tcggtacgcc
     9181 ttggataatc gccgagttga gctgctggaa ctggttgagc tgggtcggcc gtgcgcggtc
     9241 gctgaccttg accagcgtcg acagggccag catcgcgccg ctttcgctct tcacgtaata
     9301 gctactcaac cagcccgggt tatcgcggta ggcgcgctcg acctgagcaa tcaccttata
     9361 gctgcgtccg tcgatggtga aacggttgat ctcaccctcg ccaagcaggc tggccagggt
     9421 cagaccgagg tcttccatcg acacgcccat ctgtgcggct ttttcgcggt cgatttccac
     9481 caccacttcc ggcttgtcga aggccaggtc gacgtcgagg aaagcgaact ttccggactc
     9541 cactgcgcgt tgtttgaccc gttcggtcac ctgcagcagc gactggtaat cgttgggtgt
     9601 gttgatcacg aactggaacg gcagaccctc gcctgtgccc ggcaaagacg gtaggttgaa
     9661 gccgaagatc tgcaggccgg cgatttgcga caggcgtgct tgtacctccg gcagaatctc
     9721 catctgggtg cgttcgcgct cgttccacgg cgtcatcaga aagccgccga tacccgattg
     9781 cacaccgtta aagccgttga tctggaacga ggagtagtac tcagggaact ccttgaagat
     9841 ggtcacgaac tcgtcggtgt acttgttcag gtactccagg ttggtcggtt gcggcgcgct
     9901 ggccatcata aagatgatgc cctgatcttc ttccggggcc agttcgcttt tggtgaattt
     9961 gagcagcacc gggatcaggc acataacgat caccgcaaac accagcacca ccgggcgggt
    10021 atccagggtg ccgtgcaggg cgcgctggta gcgctgcttg aggctgtcga agatctgatc
    10081 gagcttgtgc gccaggcccg atgggttctc ttcatgacgc agcagcttgg cgcacatcat
    10141 cggcgacaga gtcagggcga cgatgccaga gatgatcacc gcgccggcca gggtaagggc
    10201 gaattccttg aacagcgcgc ccgtaaggcc ttcgaggaag ccgataggcg cgtacaccgc
    10261 cgccagggtg atggtcatcg acaccaccgg tacggcaatc tcccgcgcgc cttcgatggc
    10321 cgcatcgaac ggcgttttgc cttcctcgat atgccggtgg atgttctcca ccaccacgat
    10381 ggcgtcgtcc accaccaggc cgatagccag gaccatggcc agcagggtca gcaggttgat
    10441 cgagtagccc atcagctgca taaagaacat cacgccgatc atcgacaacg gaatggtgat
    10501 caccgggatc agcaccgagc ggaacgcacc gaggaacagg aacaccacca cgatgacgat
    10561 caatacggct tcgaacaggg tcttcaccac ctcatcaatc gaggcctgga taaacagcgt
    10621 ggcgtcatag gcgatggaga ctttcaggtt gggtggcagc tgcgcttcca ggtccggcaa
    10681 gaccttgcgc acttccttga tcacgtccag cgggttggcc ccaggtgtgc ccttgatggc
    10741 gatgtacacc gagggggtgc catcgaagga gctgattgag tcatagcttt ccgcgcccat
    10801 ttccacccgc gccacatcac ggatcagcac gcggctgtcg cccacggtct tcaacgggat
    10861 ggcggcaaag gcctcggccg atttcaggtc ggtttcggcg ttgatgctgg tgaccacgta
    10921 ctcgcctttc acttcgccgg ctgcagcgag gaagttgtac ttgcgcacgg cgtcattcac
    10981 atcgctggcg gtcacgccgt aggcggccat cttcaccggg tcgagccata ggcgcatggc
    11041 aaacacctga ttgccgagaa tctcggcctc ggccatgccg ggcagggtgg ccagcttggg
    11101 ctggatgacc cgcgacaggt agtcggtgat ctgcggattg ctcagctcgt cgctgaagaa
    11161 actgatgtac atcagcgctg tggagtcggc ggcttccttg ctcagtaccg ggtcttcggc
    11221 gtcctgcggc agctggttct tcacctcgtt ggccttggcc agcagttcgg tgaacaagcg
    11281 gtcgctattg gcaccgatac gcgcataaat cgagatgatc gacacgttct gccggctcac
    11341 cgaggtcatg tagtcaatac cgtcggcgct ggccaagctc tgctgcagcg gctgggtgat
    11401 atagccctga atggtctcgg cattggcccc cgggtaagcc gtggtcaccg tgatcagggc
    11461 gttttccatc tgcgggtact ggcggatggt cagcttgctg aatgcctgga agccaagcag
    11521 gattatcagc aggctgacca cggtcgctaa taccgggcga cgtataaagg gatcagtaaa
    11581 agccataaat aagttccttt gagccgtgct cgattactcg gcgttcggct tggcctcgac
    11641 attggcattg gcgatgctga cgtgggcgcc gttatccagc ttcagctggc cggcggacac
    11701 cacttgctca ccggcctgca ggcctttgag aatcaccacc tggccttcgc ggcgctcgcc
    11761 ggtttcgaca aagcggcgct cgaccaccag ctcagcctgg tctttgtcgt ctttgaccac
    11821 ctggccgtct tcagcttttt tctcgccgat cacgtacacc gagttgccat aaagggtgta
    11881 ggtgatggcg gtttccggga cgacaatcag ggtctgttcg ccgggcaaca gcacttccag
    11941 gttggcgaac atcccgggca gcaatttgtt gtccgggttg accagcatgg cgcgcacctg
    12001 cacgttgcgg gtggcgtctt cgaccttggg gttgattgcg gcgatttcgc cctcgaacac
    12061 ctcgttcggg tacgccgcca cactgaagcg cacgcgctcg ccgacagcca gtttgggcac
    12121 tgcctgttct ggcaggaaga aatcgacgta gagcttgctc aggtcctgca gggtggcgat
    12181 ggtggtgcct gaggacaggt agtcaccgac atcgatctgg cggatgccga tggtgccggc
    12241 aaacggcgca aggatgcgct tcttcgccag ttgcgcttgc aactgggcaa cgctggcgtt
    12301 ggctttttgc aggctggcgg agaggcggtc gaactcgctt ttcgagatgc tctggcgatt
    12361 gaccaggcta cgaccgcgct cgtactccac acgggccagg cccagttcgg cctgcgcagt
    12421 gcccaggctg gcctgttcga catcgctatc catctggatc agcggttgtt gcagggtgac
    12481 ctgctcgccg gagcggaaca gcacctgttg cacggtgccg ccgacctcca cggtcaggtc
    12541 aacgccctga aaggccttga gcgtaccgat ggctggcagg cgaccctgcc acgggtgttc
    12601 ttctgctgtt gcggcatcaa cgctgatagc cggctgcggc gccgagaaca tctgcacttg
    12661 ttggtagatg gagaagcctt tgtaggcggc gagggcgagt accacgacga gtactgcgcc
    12721 gagcatgatc agcatacggc ggagcaacat attccggttc cttggcaagg cggttggata
    12781 gcctgggaat aggcaagagg ggcacattag acctcgcgca caggcctgtc aaagattcgc
    12841 atttgcaaaa tattgttgtg caaaagttgc ctgattg
//
LOCUS       DASRUM010000234        24751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2137562, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000234 DASRUM010000000
VERSION     DASRUM010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 24751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24751
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2137562"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFF22_15630"
     CDS             complement(<1..215)
                     /locus_tag="VFF22_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZX18281.1"
                     /translation="MKRYEKFADQIAELIRTGMLAPGEKVPSVRHASRTYGVSPSTVF
                     QAYYLLEDRGLIQARARSGYFVRELAR"
     gene            complement(280..1695)
                     /gene="ccoG"
                     /locus_tag="VFF22_15635"
     CDS             complement(280..1695)
                     /gene="ccoG"
                     /locus_tag="VFF22_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010678608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HZX18282.1"
                     /translation="MSERIPAQIIETIDPRQPIRLTPSQAGGPIHTRSFSGRFRNLRL
                     YGAGLLFLIFFGTAWLDWDGRQAVLWNLAEHRYYIFGATFWPQDFTLLSALLIIAAFG
                     LFTITVVAGRVWCGYTCPQSVWTWVFMWAEKITEGERNQRIKLDAAPWSLNKLLRRSA
                     KHGIWLAVSLLTALTFIGYFTPIRTLSSDLLRLQLDGGTLVWVLFFMAATYLNAGWLR
                     EKVCVHMCPYSRFQSAMFDDDTLLVAYDAKRGEGRGPRKKDSNHKADGLGDCIDCYMC
                     VQVCPTGIDIRDGLQIDCISCGACIDACDSVMDKMGYARGLVGYHSERELQGGKTHWL
                     RPRLIGYGIALVLMLAAFAWALSARSMLSIDSAKDRSMFRENQLGQIENTYLLKVINK
                     TQQPQRYGLALADSPGLRLDAPQQLQLNPGEILDVPVTVVLESEQAEASALPVRFAIH
                     NLDNASEQAQTQSTFLSPRGR"
     gene            2079..3248
                     /locus_tag="VFF22_15640"
     CDS             2079..3248
                     /locus_tag="VFF22_15640"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZX18283.1"
                     /translation="MTTIVILGMLCVHLLCFCVMFLLISTRLNGKKMGMEVLALGNLL
                     LGLAYILQLLSGPSSWGFMSVVNHTLTLSAPVAYVLGAMRFFDRTTPVLRPLLVLALI
                     YSVAQVLVQLLLGDAARHALLAALCALLFLGMTAAVLYGRRSFARDLNVEMIVFAVLI
                     GGICILNAAKFVLIIDGGMAALDMSSSFQKVFYIYMSFLATVLPPCAIWLVLRRLTDE
                     LRTLAAHDPLTRLLNRRGLMDAMEAHFRSRTAGPAYLLIVDIDHFKRINDTHGHNVGD
                     LVLCHVAEVLKATARQGDLVCRLGGEEFVIIALDTDSVGAVQLAERTRTAIEYSEVPG
                     TSNSQLIRCTATIGVSDQLTSAQAFDEFLQQADAALYRGKMAGRNRVELGYAQES"
     gene            complement(3415..4119)
                     /locus_tag="VFF22_15645"
     CDS             complement(3415..4119)
                     /locus_tag="VFF22_15645"
                     /inference="COORDINATES: protein motif:HMM:NF037679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZX18284.1"
                     /translation="MSAQQTRRSASRSDSWFIYVIICRALSPALSKRKSNDSLKYDRI
                     LLQANTMSKRPVIDRDQVLNAAESIVMTRGVAALTIGEVAKAAAITKGGVQSCFGTKD
                     GLIEAMVTRWKNQYEMLVRSNLGADTSALGQLATQVKLISVPNPELSKRAAAIMAAMF
                     SQESLRKESNDWYRSQMLGQDFHSESGRSARLAFLAATGAFFLRSFDIMEISESEWED
                     LFRDIHGLLPDDSKDQ"
     gene            4186..5322
                     /locus_tag="VFF22_15650"
     CDS             4186..5322
                     /locus_tag="VFF22_15650"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE2 domain-containing protein"
                     /protein_id="HZX18285.1"
                     /translation="MGRSAESVAEPDPLCTTPFPSSHSDFIGSCFVVVVDGSMTIDRT
                     PAAGIAFAKRVRQPRILGCGLSFLFVVTANYPGTWALWGLMLVNGFIWPLIAYQLAIR
                     AASVYRAELRNLHLDCVFAGFWVVVMQFSVLPSLLLIAMAAMNCVVAKGNLFMLQGLL
                     ANALGILAGGVVFGFSITTETPSCVVWACMPMLVVYPIVVGSASYRLAQQLFSQQKAL
                     TIVSSIDEGALMSHSDWLYELAKAFHRCRCGSVQATLACIRIENFHSFHAQYGELVMN
                     ALSIRFGRLIQDGVRSSDFVSRKRPEEFWVLFLHTRRLTAQDLTKRIEERFDNFCAGS
                     EGVHGPRALVAVVEFSHRLNGEGDWMLTAEERLLGNHKSSTPTV"
     gene            5663..6412
                     /locus_tag="VFF22_15655"
     CDS             5663..6412
                     /locus_tag="VFF22_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX18286.1"
                     /translation="MKLSIRTVVLFCLLIGTFARAEPYQVVTEEWAPYNYQENNQLTG
                     LATEVVRAIMALTGDDFEMVLLPSMRATHVLQSRPKTIMYSIFRTAEREPLYKWVGPI
                     VEESIYPYQLANAPQPVTSLEQLLHARKITTRHAGLVPKMLQSLGFKNLDRSANASQQ
                     LYLMLLAGRTDIIIGDTGAGVAHYSRQLNIAPGTLRQVPIEIYRASLYIAFSLDSDDE
                     VIAAWSSALEQLRQSGELERIRRRYEQASGP"
     gene            6557..8479
                     /locus_tag="VFF22_15660"
     CDS             6557..8479
                     /locus_tag="VFF22_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003176056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX18287.1"
                     /translation="MNTWFANLSVTRKLALGFGLVLALTLALAWVAWNGLGSVIQRST
                     WMSDITQLNTTLTNLRIARLQFMLAKGDAASSDRVLSNLDIYLQQQKKLLGSFTNPVN
                     VKLLQEQDRYNQDYQRSLSGMRAAYTAADAARGKVAGETQQLGELLGAMERSVELLPE
                     YDGGRFAQLQALTRTQSELLRLRYLLERYDSAPDAQTEQALVEHLAAARASLGPLEQA
                     FGAAQQDAVRRIATALGQYQETLQAFKLATGDIARTRQEMTAQQGEIVRISEALYQFQ
                     MDRLAIESDQARSLQIAATLLAMLFGVLAAWLITRQITRPLQDTLSAVQRIADGDLTA
                     RVRVDRRDEMGQLQQGIQHMATTLRELIGGIRDGVAQIASAAEELSAVTEQTSAGANS
                     QKSETDQVATAMHEMSATVQEVARNAEQASQAANEADGQARLGDQVVGEVIAQIERLA
                     AEVSRSSEAMNGLQQESNKIGSVMDVIKAVAEQTNLLALNAAIEAARAGEAGRGFAVV
                     ADEVRGLAQRTQKSTEEIEALIAGLQNGTKQVAAAMRNSCSLTDSSVELTRKAGDSLG
                     NITHAVSNIQSMNQQIAAAAEEQSAVAEEISRSILSVRDISEQTATASDETAASSAEL
                     ARLGNQLQTLVSRFRL"
     gene            complement(8553..9077)
                     /locus_tag="VFF22_15665"
     CDS             complement(8553..9077)
                     /locus_tag="VFF22_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014339817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZX18288.1"
                     /translation="MKTMSGELRKQLIDEIIQLHTEGELTLGMAIRRLRLEVTGFDQE
                     TFAHMCGMSTRALYQLETDKGNPTLSTVDSILRKFGLRMGLMTAIAPSKRPSLVAALP
                     SNRGRVIAVKRNGVPEVVKHGDGNLKAAHPISRGAKPKGAAGNRPAVGKSHQVAKEKI
                     PEDEGSSSQGNFSQ"
     gene            complement(9074..10390)
                     /locus_tag="VFF22_15670"
     CDS             complement(9074..10390)
                     /locus_tag="VFF22_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014859433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HZX18289.1"
                     /translation="MYSALTIQTYVNGQWHDALDLSFADAQQVAGTRCTTSYCMEYLH
                     AFYDRTENIFEYAVSVNLPVNWSSDDSKGFPAFVYDIIPAGHARKSLLKRFNDEKPEG
                     MDTDLFLLARCTPSPIGNLRVKESVEALEDSGAEAFARQEVVARTNDFLEYAYEAGAA
                     LGGATGAQGEAPKLLLAEGRDGQFYADAMLNDSLVQSHWLVKFARNKVTENDKNILRA
                     EYQYYKAVSGLGLNTVATNGLSLEEAEKPSLWMPRFDRSVDGEHVERIALESVYSLLG
                     NTEPGSKLRHEAVLAKLIELWCTSGQADEVPELIFEYIRRDLLNRILGNSDNHGRNTS
                     IFRYREKFDLAPIYDLAPMVLDAETVTRATKWQAEKKGSPKWRDICACFPQWYAPDDL
                     YERIRDAAKEFRALPDLLVDMPLDIRQSNALPVNDLDRHLGYWELQ"
     gene            complement(10818..11780)
                     /locus_tag="VFF22_15675"
     CDS             complement(10818..11780)
                     /locus_tag="VFF22_15675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX18290.1"
                     /translation="MAVAMKGFHSLQTSVVKMNKPFISLCPEITRAHALKLMDWLEDE
                     RVTCYLSDSRHTSRFIEQAIDRTQLPILTHLFNRGGRFFMAYDRHDAPVGFVRLIKTC
                     ANCEIVLVIGDSDKWGRNLGASTLREGMKLAFLDMRAEKLIAKIHPDNVRSLKAFLRS
                     GFLLESESPAFKSFSMTAGRYLQLLREGAVGDSGRIYITEIDKNRLESLIALEQGPAI
                     VELEHELERAIVVKPQQVACNVVTMNSRALLQLDEEEIEVVLVYPEDADTNAGKHSVC
                     SDIGAAILGYQEGDTIDWRISDRTRRIAIRKVLYQPEAAGHFQL"
     gene            complement(12050..12601)
                     /locus_tag="VFF22_15680"
     CDS             complement(12050..12601)
                     /locus_tag="VFF22_15680"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZX18291.1"
                     /translation="MNLWFRLIWMLLHRPWRRPVPGLATTVIKLRVWPLDLDFNRHVT
                     NGRYFTLADVGRMDYVLRSGAFRVALRHRALPIVGDVWGKFRRELRLFEAFEIHTRML
                     GWDNKWSFVEHRFVKDGRVLGVVIMRGLFRGPKGNVSPAEFASELGLAEQSPQLPAWL
                     TDWSASCDQLSAQLRGEELQGAG"
     gene            12706..13677
                     /locus_tag="VFF22_15685"
     CDS             12706..13677
                     /locus_tag="VFF22_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019582014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX18292.1"
                     /translation="MHVTLLLADKCSLASASLVLELLDAANRFAEGGEQPFQVVVASI
                     DGQPVASQVGQLLQVHRSLAEITQTDLVVIPGFLFSLREVLPALAQYAPWLRQQHAQG
                     AFIAAMCTASFMLAEAGLLNGVQATTHWAFADFFRRRYPQVLLDERRILCESERLISG
                     GGASAAIDLLLHLIRRFASLELAQHCSRYLLIDNVRTEQSVYVLWSMPKQHGDEAILR
                     VQNWMEDRFDQPIQIAELAALFGFGERNFKRRFRAATGYTPLAYLQALRLERAKHLLE
                     TTQLSLESITARVGYEDSNSFRRLFQQRVGSSPTAFRKMFAQQLSGS"
     gene            13817..14230
                     /gene="arfB"
                     /locus_tag="VFF22_15690"
     CDS             13817..14230
                     /gene="arfB"
                     /locus_tag="VFF22_15690"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HZX18293.1"
                     /translation="MLIISNSVHLPDAEIELTAIRAQGAGGQNVNKVSSAIHLRFDIQ
                     ASSLSPFYKERLLALRDSRITAEGVVVIKAQQYRTQEQNRADALERLAELIRSAAKTE
                     KARRPTKPTLGSKKRRLEGKSIRGTIKAGRGKVDF"
     gene            complement(14244..15452)
                     /locus_tag="VFF22_15695"
     CDS             complement(14244..15452)
                     /locus_tag="VFF22_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016702654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX18294.1"
                     /translation="MPIALLALTLSAFAIGTTEFVIVGLLPTIATDLGVSLPSAGLLV
                     SLYALGVAIGAPLLTALTGKVPRKALLLGLMVLFTAGNLLAWQAPGYESLVIARIITG
                     LAHGVFFSIGSTIATSLVSKEKAASAIAIMFTGLTVALVTGVPLGTFIGQHFGWRETF
                     LAVSALGVIAFIGSLLYVPKNIQHNKPASVLQQLQVLKQPRLLLVYAMTAIGYGGTFI
                     AFTFLAPILQDLAGFSAGAVSLVLLVYGVSVAFGNIWGGKLADKRGPISALKIIFLLL
                     AAVLFMLTFTAHNPWLALLTVLLWGAVAFGNVPGLQVYVVRQAEHHTPHAVDVASGLN
                     IAAFNLGIAGGAWGGGLIVAHLGLIHTAWIGGLVVLVAYALTVWSGHLDRKSPLPEPS
                     SDSAAFVAGH"
     gene            15554..16456
                     /locus_tag="VFF22_15700"
     CDS             15554..16456
                     /locus_tag="VFF22_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015275069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZX18295.1"
                     /translation="MKTTLDELQAFVRVVDSGSISAAAEQLTQTASGVSRALSRLEDK
                     LDVTLLRRTTRRLELTEEGQAFLAQARKILDAVEDAEEQMKLRRQKPAGRLRVNAAAP
                     FMLHAVVPLIAGFRAQYPDIQLELHSSDQIIDLLEQRTDIAIRIGPLRDSTLHARPLG
                     SNRLRILASPTYLKAQGTPKSVEELSGHSLLGFTQPESLNQWPLRHPLGDSLSISPSL
                     SASSGETLRQLALAGAGIVCMADFMTNADRANGALVQLLLRDTVEVRQPIHAVYYRNT
                     ALASRITCFLDYLSSQLVGPGQAG"
     gene            complement(16457..17359)
                     /locus_tag="VFF22_15705"
     CDS             complement(16457..17359)
                     /locus_tag="VFF22_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003447685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX18296.1"
                     /translation="MVRRNLNDLLSFVTVAREGSFTRAAAVLGVSQSALSQAISGLEK
                     RLQIRLLTRTTRSVSPTAAGEQLLQAIGHRFDEIEEELDVLTELRDKPAGTVRITCGD
                     HVLRTTLLPRVAPLLREYPDIKVEFDVSYGLRDIVADRFDAGVRLGDTIDKDMIAVPI
                     GPELRMAAVAAPAYFAANPPPKTPADLTAHRCINMRFPTHGGLYVWEFERRGRQLNVR
                     VDGQVTLNSTPHIVVAALEGLGIAFLPEEEFAPHIEEGRLVRVLEDWCAPFAGYYLYY
                     PSRRQPSPAFALVLEALRETGVKR"
     gene            17456..17926
                     /locus_tag="VFF22_15710"
     CDS             17456..17926
                     /locus_tag="VFF22_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HZX18297.1"
                     /translation="MKKPIYLSTRWTWRQALLGSLLSLSLCGAGSALAAEELPMKIHL
                     DVDGQSVTATLDDSAAARDFAALLPLSLTLEDYAVIERIAYLPRKLVTTGAPAGVKPD
                     AGDINYYAPWGNLAIFVGGKSYDRGLVRLGRVDTGLEALHRPGPLKVRIERITD"
     gene            18063..19094
                     /locus_tag="VFF22_15715"
     CDS             18063..19094
                     /locus_tag="VFF22_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013692831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX18298.1"
                     /translation="MLAATGSSAFAAQTAAVPDMSNGADNFYTSAQVTVEKVRFKNQY
                     QMNVAGNLYMPKNFSRASAHPAIIVGHPMGAVKEQSANLYATKMAEQGFVTLSIDLPF
                     WGESEGEPRNLVAPDIYAEAFSAAVDFLGTQAFVDRARIGVIGICGSGSFVISAAKLD
                     PRMKAIATVSMYDMGAANRHALQRSLTLEQRKAIIAEAAEQRYVEFTGGETQYTSGTV
                     HQLDENTHPIQREFYDFYRTPRGQFTPKGQSPLLTTHPTLTSNVKFMNFYPFNDIETI
                     SPRPMLFIAGENAHSREFSEDAYRLAGEPKALYIVPGAGHVDLYDRVKLIPWERLTEF
                     FKQNLAAKQ"
     gene            19160..20296
                     /locus_tag="VFF22_15720"
     CDS             19160..20296
                     /locus_tag="VFF22_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX18299.1"
                     /translation="MAMCVGLLIAAEFMPVSLLTPIAADLHASNGMVGQAISVSGLFA
                     VISSLLIAGAVARWDRRHVLLGLTLIMLVSLLLIAAAPNFPVLMTARALLGVTIGGFW
                     ALATATIMRLVPAAALPKALGVLYTGNAVAVAFAAPIGSYLGGLIGWRGVFWALVPLV
                     IVNLLWQWHSLPSMPAQARSTARRVNVFGLLRRRNVAFAMLGVMLSFAGTFSTFTYLR
                     PFLEIRTGVGLSQLSFLLLGLGLAGFVGTYGASALLGRHLYRLLALLPLALAATTLAF
                     LLLGHLLWPAALLLIVWGALNAAIPVAWSTWLAQGIADEPDAGGGLLVAAIQLAIMLG
                     AGLGGVLLDHFSLSATWLGGAVMLLLGALVVGSGRRLRPGFATA"
     gene            20477..20896
                     /locus_tag="VFF22_15725"
     CDS             20477..20896
                     /locus_tag="VFF22_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZX18300.1"
                     /translation="MRFMVIVKATADSEAGFMPSEELLTAMGRYNEELVAAGVMQAGE
                     GLQPSSKGARVKFSGSQRSVTDGPFIETKELIAGFWIFQVASLAECIEWVKRCPNPMP
                     GDSEIEIRQIFEAEDFGDEFTPELREQEERLRARLES"
     gene            20936..21361
                     /locus_tag="VFF22_15730"
     CDS             20936..21361
                     /locus_tag="VFF22_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZX18301.1"
                     /translation="MKIHTYLTFDGQCEAAFTFYADCLGGELELMRMGDSPEAGNLPE
                     EYHNRVMHVCLSVGDQLLMASDSCPGMGLPYEGIKGCSVSLQVENVPEAERLYKALSA
                     GGSVQMELQATFWATRFAMFTDRFGVGWMINCDIDAQMG"
     gene            complement(21414..23681)
                     /gene="ggpS"
                     /locus_tag="VFF22_15735"
     CDS             complement(21414..23681)
                     /gene="ggpS"
                     /locus_tag="VFF22_15735"
                     /EC_number="2.4.1.213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033828 - glucosylglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerol-phosphate synthase"
                     /protein_id="HZX18302.1"
                     /translation="MLLATDLDGTFLGGDPKDRLSLYQTIAAHPEIRLAYVTGRSLES
                     VLPLLADPTLPQPDFIISDVGASLVHGDSLQPIQPLQSAVDALWPGDSQVASAIEHFN
                     LERQDVPQVRRCSYFCSPEQAANPELQEVAESLGCDLLYSAERYLDFLPKGVNKGSSL
                     QALIEWLELDNDQVLTAGDTLNDLSMLSGQFKGVCVGASEDGLLQATRQYSRVFHAER
                     AGCGGILEAFVHFGFLGKQGIAAEGKQVADPGKADLVMVYHRLPYEEFRGADGKLQRR
                     RPTSPNGIIPTLLSFFGDGRAGSWVAWAEHDENNGETFDSHTTVDAERYPRLTAARVA
                     LSKEEVDIFYKRFSKEAFWPTLHTFWERAQFREDDWQVFLKVNRAFAERTALEAAEGA
                     TVWLHDYNLWMVPGYLRELRPDLRIAFFHHTYFPSADVFNVLPWRRQIIGSLLQCDYI
                     GFHIPRQVENFVDAARGVMPLQTVSRQNCAPRFITYGCAVGLERMTTAVDTGNRVVKL
                     GAHPVGLDIERVRNALFAPKIREMMARLRTELSGVKLILSVERLDYTKGILEKLNAYE
                     RLLEENTELLGKITLVTICVPAAKEMTVYDELQGQIEQAVGRINGRFARIGWTPLQFF
                     FRSLPFEEVSAWYAMADVMWITPLRDGLNLVAKEFVAVQGLLDGRGVLVLSEFAGAAA
                     ELKGALLTNPHDPVDMTQTCYVALNMPKAEAQARLRELFDIVNYNDIRRWGDEFLAAV
                     SDPQDQLDRALGLVG"
     gene            complement(23778..>24751)
                     /locus_tag="VFF22_15740"
     CDS             complement(23778..>24751)
                     /locus_tag="VFF22_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX18303.1"
                     /translation="LYGLALVSLYMVIESWLNAQVPNDKRGQVFAVYMAVNLGALAAA
                     QQLLNLASPSDFVLFVLAAMLISAALMPITLTRQIQPSVPDTLHTNLRQIAGIAPLAL
                     AAAGLSGLALGAFWGMAPVYAALNGFDATGVGLLMSSTILGGALLQWPIGIYSDKHDR
                     RLVLFWVVALAVVVAVLMSLLPAGRLLLGLMFIWGGLAFAIYPIAVAQLIDQLHSDEI
                     LSGSSSLLMVNGIGSVCGPLLAGLLMQHLGAQALPLYFAATLGLLAAYTLYRLRHVSD
                     LVSEPAGHFMPMLRTSHTVLELMPDAPPTDSPDSTEDPLNPAPQTPG"
BASE COUNT         4945 a         7110 c         7509 g         5187 t
ORIGIN      
        1 tgccgcgcca gctcgcggac gaagtagccg gagcgggcgc gggcctggat caggccacgg
       61 tcttccagca ggtaataggc ctggaacacc gtggacgggc tgaccccata ggtgcggctg
      121 gcgtggcgca ccgagggcac cttttcgcct ggggcgagca tgcccgtgcg gatcagctcg
      181 gcaatctggt cggcgaattt ctcgtaacgt ttcatcgtgt ccgtaggggc ggccagtaga
      241 tgaccgcact ctacggggag ttgctttgcc atgcgactgt cagcgcccgc gcggtgagag
      301 aaaggtgctc tgtgtctgcg cctgctcgct ggcattgtcc aggttatgga tcgcgaagcg
      361 cacgggcagg gcgctggcct cggcctgttc gctttccagc accacagtca ccggtacatc
      421 gagaatttcc ccaggattca gctgcaactg ctgcggtgcg tccaggcgca ggccggggct
      481 gtcggccagc gccaggccat agcgctgcgg ctgctgggtc ttattgatca ccttgagcag
      541 gtaggtgttc tcgatctgcc ccagctggtt ctcgcggaac atgctgcggt ccttggctga
      601 gtcgatggac agcatcgagc gcgcgctcaa ggcccatgca aaggcggcga gcatcaacac
      661 cagggcgatg ccatagccga tcagccgtgg ccgcagccag tgggttttgc caccttgtag
      721 ttcacgctcg gagtgatagc cgaccaggcc tcgggcatag cccatcttgt ccatcaccga
      781 gtcgcaggca tcgatgcagg cgccacagct gatgcagtcg atctgcaaac catcgcgaat
      841 atcgataccg gtggggcata cctgcacgca catataacag tcgatgcaat cgcccaggcc
      901 atcggccttg tggttgctgt cttttttgcg cgggccacgg ccttcgccac gcttggcatc
      961 gtaggccacc agcagggtgt catcgtcgaa catcgcactc tgaaagcgcg agtaggggca
     1021 catatgcacg cagaccttct cgcgcagcca gccggcgttg aggtaggtgg cggccataaa
     1081 gaacagcacc cacaccagcg tgccgccgtc cagttgcagg cgcagcaggt cgctggaaag
     1141 tgtgcggatc ggcgtgaagt acccaataaa ggtcagcgcc gtaagcaggc tgactgcgag
     1201 ccagatccca tgcttggcac tgcgccgcag cagtttgttt agcgaccagg gcgctgcatc
     1261 tagcttgatt cgctggttgc gctcgccctc ggtgattttt tcggcccaca taaacaccca
     1321 ggtccacacg ctttgcgggc aggtgtagcc acaccacacc cgcccagcca ccacggtgat
     1381 ggtaaacagg ccaaaggccg caatgatcag tagtgccgag agcagggtga aatcctgcgg
     1441 ccagaaggtc gcgccgaaga tgtaatagcg atgctcggcg aggttccaca gcaccgcctg
     1501 gcgaccgtcc cagtccagcc aggcggtgcc gaagaagatc agaaacagca agccggcacc
     1561 gtacaggcgc agattgcgaa aacgcccgct aaaactgcgg gtatggatcg gtccgccggc
     1621 ctgggaaggc gtcaggcgga taggctggcg cgggtcgatg gtctcgatga tctgcgcggg
     1681 aatacgttcg ctcattacgg gcctcgtcgg gctttatcag gcgaggcgaa tgatgcgagc
     1741 gcagctgttt ggataacaga ctcaggtttt caaataaaaa gcggatcaga tagtcagggc
     1801 agtggtaatg gtggcgagtc attatcagag ctttgggggc agcgcggata ctggctatga
     1861 gcctgtgctg cctcagtcgt tacgctactg acacaagcct gtcgctttga aacatcaggc
     1921 tgaggaccaa aactactgtg gctatggcaa gcaccagcac tagtgttaaa tagccatacc
     1981 aaacatctag ggagtactga gggatcccag caagcgtgcg cttttgctac ccctttgtgt
     2041 ttctccgtta ttgcgatttt tctatatacg cgacgcctat gaccaccatc gttatccttg
     2101 gcatgctctg cgtgcactta ctctgctttt gcgtgatgtt cctgctgatc agcacccggc
     2161 tgaatggcaa gaagatgggc atggaggttt tggcactcgg caatcttctg cttggacttg
     2221 catatattct tcaattgctc agcggcccct cgagttgggg gttcatgagt gtcgtcaacc
     2281 atacgctgac cctcagcgct cctgttgcgt atgtcttggg cgccatgcgc ttttttgatc
     2341 ggacaacccc tgttttgcgc ccactgctcg tattggctct tatctactcg gtggcgcagg
     2401 tactggtgca attgttgctc ggggacgcgg cccgtcatgc attattggcg gccttgtgcg
     2461 ctttgctctt tctgggcatg acagcggcag tgctctatgg caggcgaagt tttgccaggg
     2521 acctgaacgt tgaaatgatc gtgtttgccg tgctgatcgg cggcatctgc atcctcaatg
     2581 ccgcgaagtt tgtgttgatt atcgatggcg gaatggcggc gctcgatatg agcagcagct
     2641 tccagaaggt cttttatatc tacatgtcgt tcctggcgac cgtactacca ccttgcgcga
     2701 tttggctggt gctgcgccgc cttacggatg aactgcgtac cttggccgca catgatccgc
     2761 tcaccaggct gttgaaccgg cgtgggctga tggatgcaat ggaagcgcac tttcgctcac
     2821 gcactgccgg gcccgcgtac ctgctgatcg tcgacatcga ccacttcaaa cgtattaatg
     2881 acacccatgg ccacaacgtc ggcgatcttg tcctttgcca cgttgccgaa gtcctcaagg
     2941 ccaccgcccg gcagggcgat ctggtctgca ggctgggcgg cgaggagttc gtgattatcg
     3001 ccctggatac cgacagcgtt ggtgccgtgc aactggcaga gcgcacacgg acggcaattg
     3061 aatacagcga agtacctggc accagcaaca gccagttaat ccgctgcacc gcaaccattg
     3121 gagtctcgga tcagctcacc agcgcgcagg cgttcgacga gtttttgcag caggcggatg
     3181 ccgcgctata ccgaggcaag atggcagggc gcaaccgggt tgagttggga tatgcgcagg
     3241 agagttagaa ggccatgcac gaccttcaga ttggtttgaa tggaaatcca tccactgaaa
     3301 actggatagg tttaatttag atggaagggg ttggaggggc ggagtgttgt atcgagtggt
     3361 tatcggccta aagcagatta tttgccaaat aactaaatct atcccccttt ccgcttattg
     3421 gtcctttgaa tcatccggta gcaggccatg aatatctctg aacaagtcct cccactcgct
     3481 ctcagaaatt tccatgatgt caaacgagcg caagaaaaac gcacctgtag cggctaggaa
     3541 tgctaagcgt gcgctcctcc ctgactcgga atgaaagtcc tgccccagca tttgcgatct
     3601 gtaccaatca ttcgactcct ttcggaggct ctcttggcta aacatggcgg ccatgatggc
     3661 ggcagcacgt ttgctgagtt cgggattcgg tactgagatg agtttgacct gcgtagccag
     3721 ttgtccgagc gcgctggtgt cggcaccgag gtttgaccgc acgagcattt cgtactggtt
     3781 tttccagcgg gtgaccatcg cttctatcag tccatccttg gtgccaaaac aagactgtac
     3841 accgcccttg gtgatggctg ctgccttggc aacttcgcca atagtcaggg ccgcgacacc
     3901 tcgcgtcatg acaatagatt ctgctgcgtt cagaacctga tctctgtcta taactgggcg
     3961 tttactcatt gtgttggcct gcaatagaat acgatcgtat tttaatgaat cgtttgattt
     4021 ccgctttgaa agcgccgggg ataacgctcg gcaaattatc acgtatatga accaggaatc
     4081 ggaacgtgag gcggagcgtc gtgtttgttg cgcgctcatt cagctgcgtg ctcaacctgc
     4141 agactttccg cccctccagc atctgacagc ctgccggtat atgccatggg ccgcagtgca
     4201 gaatctgtag ccgaacctga tccactttgt acaacgccat ttccctcttc ccattcggat
     4261 ttcatcggca gctgttttgt tgtcgttgta gacggttcta tgactattga tcgaacgcca
     4321 gcagccggca ttgcctttgc taaacgtgtc cgccaacccc gcatcttggg ctgcgggttg
     4381 agcttccttt ttgtggtaac agctaattac ccaggtacct gggcgctttg ggggttgatg
     4441 ctggttaacg gcttcatttg gccgcttatc gcatatcagt tagccattcg cgcagcgtcg
     4501 gtttatcgag cggaactcag gaatcttcac cttgattgtg tatttgccgg gttttgggtg
     4561 gttgtcatgc agttcagcgt actcccctcg ctactgctta tagcgatggc tgccatgaac
     4621 tgcgttgttg ctaaaggcaa cctcttcatg ctgcaaggac tcttagcgaa tgcactgggt
     4681 atattggcag gaggtgttgt attcggtttt tccatcacca cggaaacacc gagctgtgtg
     4741 gtatgggcgt gcatgccaat gctcgtggtc tacccaatag tcgtcggctc ggcaagctat
     4801 cgcttggctc aacagctttt ctcacaacag aaagccctca ctattgtgtc cagcatcgat
     4861 gagggggcgc tgatgtcaca tagcgactgg ctctatgaac tggccaaggc gttccatcgc
     4921 tgtcgatgtg gctcggttca agccactttg gcgtgcatcc gcattgaaaa tttccactcg
     4981 ttccacgcgc agtatggtga gttagtcatg aatgcactga gcattcggtt tggacggctg
     5041 attcaggacg gtgtgcgcag ctcagatttt gttagccgca agcgtcccga agagttctgg
     5101 gtactgttcc tccacactcg tagattaact gcgcaggatt taactaagcg aattgaagag
     5161 cgtttcgaca atttctgtgc tggctccgaa ggggttcatg gtccacgagc tttggttgcg
     5221 gtagtagagt tctcacatcg tttgaatggg gagggggatt ggatgctgac agccgaagag
     5281 cgcctgttag gcaaccataa gtccagcact cccaccgttt agtaaccgac agtccagatt
     5341 attgattgat cgttttttgt cagcactggg gccgcaagtt acggtgagat gaatcggcct
     5401 gccggcctcg ccgggcttta tcaggcgagg cgaatgatgc gagcgcagct gtttggataa
     5461 cagagtcagg ttttcaaata aaaaacggat cagatgaagg tggctgagat ggggcatatt
     5521 caacatgtaa ccctgtgctg cagtatcggt cccacgctca gtaggacagc ttcaggtgtt
     5581 agacgcgtgt cagtatctgt gcttttcttg ctcctcaatc agacttctcc ttgggtacag
     5641 cgcgacccat caggcagagc atgtgaaact ttcgatacgc actgttgttc tgttctgtct
     5701 cctgatcggc acctttgccc gtgccgagcc gtatcaggta gtgactgaag agtgggcgcc
     5761 atacaactac caggagaaca atcagctcac cggccttgcc actgaagtcg ttcgggccat
     5821 catggcgttg accggggatg actttgagat ggtgctgttg cccagtatgc gagccaccca
     5881 cgtattgcag agccggccaa agaccatcat gtattccatc ttccgcacgg cggagcgcga
     5941 gccgttgtac aagtgggtcg ggccgatcgt agaggagtcc atttacccct atcagctggc
     6001 taatgcgccg cagccggtga cctctctgga acaactgctg cacgccagaa aaataactac
     6061 gcgacatgcc ggcctggtgc ccaaaatgct gcagtcgcta ggcttcaaaa atctcgacag
     6121 aagtgcgaac gcgagccagc agctctacct catgctgctg gctgggcgca ccgacatcat
     6181 catcggcgac accggtgcgg gggtggctca ctacagccga cagttaaaca tcgctcccgg
     6241 cacgctgcgg caggttccaa tcgagatcta tcgtgcatcg ctgtatatcg ctttcagcct
     6301 cgacagtgac gacgaagtaa ttgccgcctg gagcagcgct ctggagcagc tgcgccagtc
     6361 gggcgagctg gagcgcatcc ggcgtcggta tgagcaagcc agcgggccgt aaacgtgagc
     6421 caaccgcagt aaacaccgta aaagtgtgcc gaaatcccgt gttagtgcgt cagcacaggc
     6481 cttgtgctgg cttcaacaaa aacaattctc cagcgaaacc tgaactgcct gtcttaacga
     6541 cagttggaga atttttatga acacctggtt tgccaacctt agcgttaccc gcaagctggc
     6601 cctcggcttc ggtctggtgc tggcgctgac tcttgccctg gcctgggtcg cctggaacgg
     6661 cctgggcagc gttatccagc gcagcacctg gatgagcgat atcacccagc tcaacaccac
     6721 cctgaccaat ctgcgtattg cccgcctgca gttcatgctg gccaagggtg atgccgcaag
     6781 cagtgatcgc gtgctgagca acctcgatat ctatttgcaa cagcagaaga agctgctcgg
     6841 cagctttacc aatccggtca acgtcaagct gttgcaagag caggaccgtt acaaccagga
     6901 ttaccagcgc tcgctgagcg gcatgcgcgc cgcctacacg gcggctgatg ctgcgcgggg
     6961 caaagtggcc ggcgagacgc aacagctcgg cgagctgctg ggcgcgatgg agcgcagcgt
     7021 ggagttgctg ccggaatacg acggcggtcg ttttgcccag ttgcaggcgc tgacccgtac
     7081 ccaaagcgag ctgctgcgcc tgcgctacct gcttgagcgt tacgacagcg cccctgatgc
     7141 gcaaaccgag caggcgctgg tcgagcatct tgccgccgcc cgtgccagcc tagggccgtt
     7201 ggagcaggcc ttcggcgctg cccagcagga tgcagtacgc cgcatcgcca cagccttggg
     7261 tcagtatcag gagacgctgc aagcgttcaa gctggccacc ggcgatattg cccgcacccg
     7321 tcaggaaatg accgcccagc agggcgagat cgtgcgcatc agcgaggcgc tgtaccagtt
     7381 ccaaatggac cgcttggcca tcgagagcga ccaggcgcgc agcctgcaga tcgccgccac
     7441 gctgctggcg atgctcttcg gcgtgttggc cgcttggctt atcacccggc agatcacccg
     7501 tccgttgcag gacaccctga gcgcggtgca gcgcatcgcc gatggtgacc tgaccgccag
     7561 agtgcgggtc gaccgtcgcg atgagatggg ccagctgcag caaggtatcc agcatatggc
     7621 cacgaccctg cgcgagttga ttggtggcat ccgtgatggc gtggcgcaga tcgccagcgc
     7681 tgccgaggaa ctgtccgcag tgaccgaaca gaccagcgcc ggtgctaata gccagaaaag
     7741 cgagaccgac caagtggcca ccgccatgca tgagatgtcg gccaccgttc aggaggtggc
     7801 gcgcaacgcc gaacaggctt cccaggccgc caacgaagcg gacggtcagg cgcgcctggg
     7861 cgaccaggtg gtgggcgagg tgatcgccca gatcgaacgc ctggccgccg aggtcagccg
     7921 ctccagtgag gcaatgaatg gcctacagca ggagagcaac aagattggca gcgtgatgga
     7981 cgtgatcaag gcggtggccg agcagaccaa tctgctggcg ctcaatgcgg ctattgaggc
     8041 tgcgcgcgct ggcgaggccg ggcgcggttt tgccgtggtc gccgatgagg tgcgcggcct
     8101 ggcccagcgc acgcagaaat ctaccgaaga gatcgaggcg ctgatcgccg gcctgcagaa
     8161 cggtaccaag caggtggccg cggccatgcg caacagctgt agccttaccg acagtagcgt
     8221 cgaactgacg cgcaaggccg gcgattcact gggcaacatt acccacgcgg tgtccaatat
     8281 tcagtcgatg aaccagcaga tcgccgcagc cgccgaggag cagagtgcgg tggccgagga
     8341 aatcagccgc agcatcctca gcgtgcgcga tatctctgag cagactgcca ccgccagcga
     8401 cgaaaccgcc gcctccagcg ccgagctggc gcgcctgggc aatcagctgc aaaccctggt
     8461 tagccgcttc cgcctgtagt gcaggcagct tgaggccgtg gctgttagcc acggccttcg
     8521 gcatgtgcgg cctctcgatg gctctactcg cactactgac taaaattgcc ctggctgctt
     8581 gaaccctcgt cctctgggat cttctctttg gcaacttggt gcgacttgcc aactgcaggt
     8641 ctgttcccag cggccccttt aggctttgct cccctcgaga tggggtgggc ggccttaagg
     8701 ttgccgtccc catgcttaac aacttcgggt acgccattgc gtttaaccgc aataacgcgc
     8761 cctctgtttg agggcagcgc agccactagc gatggtcttt tgctgggtgc aatggccgtc
     8821 ataaggccca tccgcaggcc aaatttgcgc aatatcgaat cgacggtgct caaggtcgga
     8881 ttacctttgt ccgtttccag ctgatacagc gctcgagtgg acatgccgca catgtgggca
     8941 aaggtttcct gatcaaaacc tgtgacctcc aagcgcagcc tacggatagc catgccgagg
     9001 gttaattcac cctcagtgtg tagttgaatg atctcgtcga tcagttgctt gcgcagttct
     9061 ccgctcatgg ttttcattgc agctcccaat acccaaggtg cctgtccaga tcgttcacgg
     9121 gcagcgcatt tgattggcga atatccaacg gcatatcgac gagtaaatcc ggcagcgccc
     9181 tgaattcttt ggcggcatca cggatacgct cgtacagatc atccggggca taccactgcg
     9241 ggaaacaggc gcagatatca cgccacttcg ggctgccttt cttttcggct tgccatttcg
     9301 tcgccctggt taccgtctcg gcatccagaa ccatcggggc caggtcatag atcggggcca
     9361 gatcaaactt ttcccggtag cggaaaatgg atgtgttgcg accgtgattg tcactgttgc
     9421 caaggattcg attgagcagg tcgcgacgga tgtactcaaa gatcagctcc ggaacctcat
     9481 cggcttgtcc gcttgtgcac cacagctcaa tcagcttcgc cagcacagct tcatgcctga
     9541 gcttcgaccc gggctcggtg ttccccagta aggaatagac cgactcaagc gcgatgcgct
     9601 cgacgtgctc gccgtccacg ctgcggtcaa aacgtggcat ccaaaggctg ggtttttcag
     9661 cttcttccag gctcaaacca ttggtggcaa cggtgttcaa acctaggccc gataccgctt
     9721 tgtagtactg atattcggcg cgcagaatgt tcttgtcgtt ctcagtcacc ttgttccggg
     9781 cgaacttgac caaccagtgc gattgcacga gcgaatcatt gagcatggcg tccgcataaa
     9841 actgtccgtc tcggccctct gccagtaata gcttgggggc ttcaccctgt gcgcctgtcg
     9901 caccgcctag ggctgcgcca gcctcgtaag cgtactcaag aaaatcattg gtgcgcgcaa
     9961 caacttcctg gcgagcaaag gcctcggctc ccgagtcctc aagtgcttca acagactctt
    10021 tcacacgcag attgccgata ggcgagggcg tgcatctagc caacaagaac agatcagtat
    10081 ccatcccctc aggcttctca tcattgaaac gcttgagcag cgacttcctt gcatgcccag
    10141 ccggaatgat gtcgtaaacg aaggctggaa agcctttgct atcgtcagag ctccagttga
    10201 ctggcaagtt gacgctgacg gcatattcga agatgttttc cgtccggtca taaaaggcat
    10261 gcaggtattc catgcagtac gaagtcgtac agcgcgtacc cgcaacctgt tgcgcatccg
    10321 caaagctcaa atcaagggca tcgtgccatt gcccgtttac gtaggtttgt atggtcagcg
    10381 cgctgtacat ggcgcaacct caatcggcat taagatgctg ataacgctag atcatggtgg
    10441 cttaatcggc aatataaatc ttgaagtagg gtctttcttg ttttagttcg gcactttaat
    10501 gctggttgtg atttgtctga tgtggctgta gcgatggctt attagcaggg gttttagtct
    10561 ccgcgaggga gtggaagcag ccattgctga atgagtgctt ctggttgatg gtaggcagtc
    10621 atgcaggact gcttttggtc aatgaagcca ctcagacgtg tttaggtagg ctggggcgat
    10681 tcaagtggcc tcctgatgct aaagcttgcg tcttagtttc tagccaagtc agcagtccgc
    10741 atcagacacg gaaagcgagg ttacgccctt tggagaatcc tcgcgtctaa tggacgaaca
    10801 ctaaactaaa gacacaatca tagttggaaa tggcccgctg cctcaggctg gtaaagcact
    10861 ttcctgatcg caatcctgcg ggtccgatca gaaattcgcc aatcgattgt gtccccttcc
    10921 tgatagccca ggatggcagc tccgatgtcg gaacaaacgg aatgtttccc tgcattggtg
    10981 tctgcgtctt ccgggtagac caaaaccact tcgatctctt cttcgtccag ctgcagcaag
    11041 gccctagagt tcatcgtgac gacattacac gcgacctgct gtggcttgac gacaatggct
    11101 cgttcaagct catgttcgag ttcaacaatg gccgggcctt gctcgagcgc gatcagactt
    11161 tcgagcctgt ttttgtcgat ttcagtgatg tagatcctgc cggagtcgcc aacggcacct
    11221 tcgcgcaaga gctggagata gcgccctgcc gtcatggaaa atgacttaaa tgcaggcgat
    11281 tcgctctcaa gcaggaagcc gctgcgcaga aaggctttta gcgagcgcac gttatccggg
    11341 tggatcttgg cgatgagctt ttcggcccgc atgtcgagaa acgccagttt catgccttcg
    11401 cggagggtgc tggcgccaag gttgcggccc catttgtcgc tgtctccgat gaccaggact
    11461 atttcgcaat ttgcgcaggt cttgataagg cggacaaagc ccaccggagc gtcatgtcgg
    11521 tcataggcca tgaagaatcg gccaccccgg ttgaatagat gggtcaggat cggtaactga
    11581 gtacgatcga tggcttgctc gatgaagcgg gaggtatgac gcgaatcgct caggtagcag
    11641 gtgacgcgct catcctccaa ccagtccatc agcttcagcg cgtgtgcccg agtaatttca
    11701 gggcacagcg aaatgaaagg cttgttcatc ttcaccacac ttgtctgtaa agaatgaaag
    11761 cccttcatgg ctacggccat gacggttctt tcggcagccg gccatcttaa gacataacgc
    11821 gggtaatgcc gaattgccta ggcagcctct tcagaacagc agcgagtgca cttgtgcacg
    11881 agtcttgatt tcaagctctt cgatgtacag gcgcagcaga gctgtgaatc gcctcgggtt
    11941 tcgtaggctc atccgaaggt ccgctcctgg ccgacacctg ccaaacacca ccgccagctc
    12001 tggctaagcc ccctccaaat gaccgccatc cgcccccagt ggaggctgat catccagccc
    12061 cttgcagttc ttccccgcgc agttgagcgc ttagttggtc gcagcttgcc gaccagtcgg
    12121 tcagccaggc tggcagttgc ggggattgct ctgccaggcc gagctcactg gcgaattcgg
    12181 cgggggagac gttgcccttt gggccacgga acaggccgcg catgatgacc acgccgagca
    12241 cgcgaccatc cttgacgaag cggtgttcga caaaactcca cttgttatcc caacccagca
    12301 tgcgggtgtg gatttcgaag gcttcaaaca gcctcagttc acggcggaac ttgccccaaa
    12361 cgtcgccaac gataggcagc gcccggtggc gcagggcaac ccggaaggcc ccgctgcgca
    12421 gcacataatc catgcgcccg acatctgcca gggtgaaata acggccgttg gtgacatgcc
    12481 ggttgaagtc gaggtccagt ggccataccc gtaacttgat aaccgtggta gccaggcctg
    12541 gcaccgggcg tcgccagggg cggtgcagta gcatccatat aagacgaaac cagagattca
    12601 taaacaggca cctgcgagag agagggcgca ctctagtgag caactttgcg ctaaggtaga
    12661 tgcagaaatg acttatttca tgcgaaaagt gcaagggagc cctgtatgca tgtaacgctt
    12721 ctgcttgccg ataagtgctc gcttgctagc gccagtctgg tgctggagtt gctggacgct
    12781 gccaatcgct ttgccgaagg cggcgagcaa ccttttcaag tggttgtcgc ctctattgat
    12841 ggtcagccag ttgcaagcca ggttgggcag ttgctgcagg tgcacaggtc gctggccgag
    12901 atcacacaga ccgatctggt ggttatccca ggctttcttt tcagcctgcg ggaggtcttg
    12961 ccggcattgg cgcagtacgc cccttggttg cgccagcagc atgcgcaggg cgcttttatc
    13021 gctgcgatgt gtacggccag ttttatgctt gccgaagcag gcttgctcaa tggcgtgcag
    13081 gccaccactc actgggcttt tgccgatttc ttccgccgcc gctatccgca ggtgttgctt
    13141 gatgagcggc gtatcctgtg cgagagcgag cgcctgatca gcggcggtgg agccagtgcg
    13201 gcgatagacc tgctgttaca cctgatccgt cgtttcgcct cccttgagct agctcagcac
    13261 tgcagtcgct atctgcttat cgacaatgtg cgcactgagc agtcggtcta tgtgctctgg
    13321 tcgatgccca agcagcacgg cgacgaggca atcctgcggg tgcaaaactg gatggaggat
    13381 cgtttcgatc agcccatcca gattgccgag ctggcggcgc tttttggttt tggtgagcgc
    13441 aacttcaagc ggcgtttccg tgcggcaacg ggctacaccc cgttggctta tctgcaggca
    13501 ttacgcctgg agagggccaa gcatctgctg gaaacgaccc agctcagcct ggaaagcatc
    13561 accgccaggg ttggttatga ggacagcaac tcgtttcgcc gcctgttcca gcagcgcgtt
    13621 ggctcctcgc cgacggcgtt ccgcaagatg ttcgcgcagc agctgagcgg ttcttgaatc
    13681 caggaaaacg ccaggcaatg gcttgattgc gattcgggtt acagggctgc ctctttccat
    13741 atgccaggca aatccgctat ttgataagac gggcgagtta tcctgtgccc catcgttact
    13801 cttggttcct catagcatgc tgatcatttc caacagcgtt catctgcctg acgctgaaat
    13861 cgagctgacc gccatccgcg cccagggtgc gggtgggcag aacgtcaata aggtgtccag
    13921 cgcgatacat ctgcgcttcg acatccaggc ttccagcttg tcgccgttct acaaggagcg
    13981 gctgctggca ttgcgcgaca gccgcatcac cgccgaaggt gtggtggtta tcaaggccca
    14041 gcagtaccgc acccaggagc agaaccgtgc cgacgcgctg gagcgtctgg ccgagctgat
    14101 tcgcagcgcc gctaaaaccg aaaaagcccg ccgcccgacc aagccgaccc tgggttcgaa
    14161 gaagcgccgc ctggaaggca agagcatccg tggcacgatt aaggcagggc gcggcaaggt
    14221 cgacttctag actccgagtg ggatcagtgc ccagcgacga atgcggcgct gtcgctgctc
    14281 ggttctggca gtgggctctt gcggtccaga tggccgctcc agacggtcag ggcgtaggcc
    14341 accagcacca ccaggccgcc gatccaagcg gtgtggatca gccccagatg cgcgacgatc
    14401 aggccgccac cccaggcacc gccggcgatg ccgaggttga aggcggcgat attcagaccc
    14461 gaggcaacat cgactgcgtg cggcgtgtga tgttcggcct ggcgcaccac atacacctgc
    14521 aggcccggca cgttgccgaa ggccaccgcg ccccacagca gcacggtcag cagcgccagc
    14581 cacgggttgt gggcagtaaa ggtgagcata aacagcacgg cggcgagcag cagaaagatg
    14641 attttcagtg cgctgatcgg cccgcgttta tctgccagtt tgccgcccca gatattgccg
    14701 aaagccacgg acacgccata caccagcaac accaggctga ctgcgcccgc gctgaagccg
    14761 gcgaggtcct gcaggatcgg cgctagaaag gtgaaggcga taaaggtgcc gccgtagccg
    14821 atggcggtca tcgcatacac caacagcagg cgcggctgct tgagcacctg cagctgctgc
    14881 agcaccgaag ccggtttgtt gtgctgaatg ttcttcggca catacagcag gctgccgata
    14941 aaggcgatca cccccagggc ggatacggcg aggaaggttt cgcgccagcc gaagtgctgg
    15001 ccgataaagg tgcctagcgg cacgccggtg accagcgcca cggtcaggcc ggtgaacatg
    15061 atggcaatcg cgctggcggc tttttccttg gacaccaggc tggtagcgat ggttgagccg
    15121 atggagaaga acaccccgtg ggccaggccg gtgatgatcc gtgcgatcac cagtgattca
    15181 taaccaggtg cttgccaggc cagcaggttg ccggcggtaa acagcaccat caaaccgagc
    15241 agcagggcct tgcgcggcac cttgccggtg agtgcggtaa gcagcggcgc gccgatggcc
    15301 acgccgaggg cgtagaggct gaccagcagg ccggcggagg gcaggctgac gccaaggtcg
    15361 gtggcgatgg tgggtagcag accaacgata acgaactcgg tggtgccaat ggcgaaggcg
    15421 ctgagtgtaa gcgccaatag agcaatgggc atggcaggga ctccaaggga aactgtgaga
    15481 tgtgcagtct ccccggatca ctcgtgcgta aaaatagggt tttcagcaca agatatttga
    15541 ctaatatgca cgaatgaaaa cgaccctgga tgaactacag gcctttgttc gcgtggtgga
    15601 cagcggttcg atcagtgccg ccgccgagca gctgacgcag accgcctctg gcgttagccg
    15661 cgccctcagc cggctggaag acaagctcga tgtcaccctg ctgcgccgca ccacgcgccg
    15721 cctggaactg accgaggagg gtcaggcctt tctggctcag gcgcgcaaga ttctcgatgc
    15781 ggtggaggat gccgaggagc agatgaagct gcggcggcag aagcccgccg gacgcttgcg
    15841 ggtgaatgcc gctgcgcctt ttatgctgca tgcggtggtg ccattgatcg cggggtttcg
    15901 tgcgcagtac ccggatattc agctggaact gcacagcagc gaccagatca tcgacctgct
    15961 ggaacagcgc accgatatcg ccatccgcat cggcccgctg cgcgattcga ccctgcatgc
    16021 gcggccgctg ggcagtaatc gcctgcgcat tctcgccagc ccgacctatc tcaaggccca
    16081 gggcacgccg aaaagtgtcg aggaactgag cgggcacagc ctgctcggtt ttacccaacc
    16141 ggagagcctt aatcagtggc cgctgcgtca tcctttgggt gattcgttga gcatcagtcc
    16201 aagcctaagc gcttccagtg gcgaaacttt gcgccaactg gcgttggccg gagcagggat
    16261 tgtctgcatg gcggacttta tgaccaatgc cgaccgcgcc aacggcgcgc tggtgcaact
    16321 gctgctgcgc gataccgtcg aggtgcgtca gccgattcac gcggtgtact accgcaacac
    16381 cgcactggca tcgcgtatca cctgttttct cgattacctg agcagtcagc tggtcgggcc
    16441 tgggcaggcg ggttgatcag cgcttaacgc cggtttcgcg cagcgcctca agcaccaggg
    16501 caaacgccgg ggagggctgt cggcggctgg ggtaatacag gtaatagccg gcgaagggcg
    16561 cgcaccagtc ctccagcacg cgcaccagac ggccttcctc gatatgtgga gcgaactctt
    16621 cctccggcag gaaggcaata cccagcccct ccagcgccgc gacgactata tgcggtgtgc
    16681 tgttgagcgt gacctgacca tcgacacgca cattcaactg ccggccccga cgctcgaact
    16741 cccagacata cagcccgcca tgggtcggaa agcgcatgtt gatgcaacgg tgtgcggtca
    16801 gatcagcagg ggtttttggg ggcgggttcg ctgcgaagta ggcgggcgcg gccacggcag
    16861 ccatgcgcag ctcggggcct atgggcacgg cgatcatgtc cttgtcgatg gtatcgccca
    16921 ggcgcacgcc agcatcgaag cggtccgcaa cgatgtcgcg caacccgtaa ctgacatcga
    16981 actccacctt gatatcggga tactcgcgca gcagcggcgc gactctcggc agcagcgtgg
    17041 tgcgcagcac gtggtctcca caggtgatgc gcacggtgcc ggccggcttg tcgcgcagtt
    17101 cggtgagtac gtccagctcc tcttcgatct cgtcgaaacg atgaccgata gcctgcagca
    17161 gttgctcgcc agccgccgtg ggcgaaacgc tgcgggtggt gcgggtgagc aggcgaatct
    17221 gcaggcgttt ctccaggccg ctaatggcct ggctcagcgc cgactggctg acgcccagca
    17281 cggcggcggc gcgggtaaaa ctgccttccc gcgcgacggt cacgaaggac aacagatcgt
    17341 tgaggttacg tctgaccatg gtggattctt tcggcgttat tgattagtcc tgcttataac
    17401 ctctttaagg attcattagc tagttgcgcc tgtcgctatt cgtaagaatg ccgttatgaa
    17461 aaagcccatc tatctctcca ctcgctggac ctggcgtcag gcactgcttg gcagcctgct
    17521 gagtctgtcg ctatgtggcg ctggcagtgc tctggctgcc gaggagttgc ctatgaaaat
    17581 tcatctggat gtcgatggcc aaagcgttac cgcgacgctg gacgacagcg ccgctgcccg
    17641 tgatttcgcc gcactgttgc cgctctctct gacgcttgag gactacgccg taatcgagcg
    17701 tatcgcctac ctgccgcgca agctggtcac cactggcgca ccggccgggg tgaagcctga
    17761 cgcgggcgac atcaactact acgcaccctg gggcaatctg gcgatttttg tcggcggcaa
    17821 gtcctatgac cgcggcctgg tgcgcctggg cagagtggat acgggccttg aggcgttgca
    17881 ccggccgggg ccgctaaagg taaggatcga gcgcattacg gactgacgtc ccgcaactta
    17941 aatcgcgggc ggtttgccgt ctgcctggaa cacccaatcc tggagatttc accatgagca
    18001 aacaaaaaac cgagcggcgc gactttctca aagcggtggg caccggcgta ttcgcgctga
    18061 gcatgttggc ggccacgggc agttcggcat ttgctgcgca aacggcggca gtgccggata
    18121 tgtccaacgg tgcagacaac ttctacacca gcgcgcaggt gacggtggag aaggtccggt
    18181 tcaaaaacca gtaccagatg aacgtcgccg gcaatctcta tatgccgaag aacttcagcc
    18241 gcgctagcgc ccatccggcg attatcgtcg gacacccaat gggtgcagtg aaggagcaga
    18301 gcgctaatct ttacgccacc aagatggcgg agcagggctt tgtgaccctg tccatcgacc
    18361 tgccgttctg gggcgagagc gaaggtgagc cgcgcaacct ggtggcaccg gatatctacg
    18421 cggaagcatt cagcgcagcg gtggattttc tcggcacgca ggcctttgtc gaccgggcgc
    18481 gtatcggggt gattggtatc tgcggtagcg gcagctttgt gatcagcgcg gccaagctcg
    18541 acccgcgtat gaaggccatc gccacggtca gcatgtacga catgggcgcg gccaaccgcc
    18601 atgcgctgca acgctcactg accctggaac agcgcaaggc gatcattgcc gaggcggctg
    18661 agcagcgtta tgtggagttc accggcggcg agacgcagta caccagcggc accgtgcacc
    18721 aactggatga aaatactcac cccattcagc gtgagttcta tgacttctac cgcacgccac
    18781 gcggccagtt cacgcccaaa ggccagtcgc cgctgctgac cacgcaccca acgctgacca
    18841 gtaacgtcaa attcatgaac ttctatccgt ttaatgacat cgagacgatt tcaccgcggc
    18901 cgatgctatt tatcgcgggt gagaatgcgc attcacggga gttcagcgaa gacgcttacc
    18961 gcctggctgg tgagccgaaa gccttgtata tcgttccggg tgccggccac gttgatctgt
    19021 acgatcgggt aaagctgatc ccttgggagc gtctcaccga gttcttcaag caaaatctgg
    19081 ctgctaagca gtgactgcag ttgttacgac actatccgca gcggctgcca cacgacacat
    19141 gtggagtgcg gtcggctcga tggccatgtg tgtaggcttg ctgatcgccg cggagtttat
    19201 gccggtcagc ctgctgacac ctattgccgc cgacctgcat gccagcaatg gtatggtcgg
    19261 gcaggcgatt tctgtgtcgg ggttgtttgc tgttatcagc agtctgctga ttgccggtgc
    19321 ggtggcccgc tgggatcgcc gtcatgtgtt gctggggttg acgctgatca tgttggtctc
    19381 gctgttgctg attgcggcag cgcccaactt cccagtactg atgactgctc gggcgttgct
    19441 gggcgtgacc atcggcggct tctgggcgct ggcgacggca accatcatgc gcctggtgcc
    19501 ggcggcagcg ctgcccaagg cgctcggggt gctctatacg ggcaacgccg tggcagtggc
    19561 gtttgccgcg ccgattggca gttacctggg cgggcttatc ggctggcgtg gggtgttctg
    19621 ggcactggtg cctttggtta tcgtcaatct gctgtggcag tggcacagcc tgccatcgat
    19681 gcctgcgcag gcgcgctcaa cggcgcgtcg cgtcaatgtg tttgggctgc tgcggcgacg
    19741 caatgtcgct tttgccatgc ttggcgtgat gctctcgttc gcggggacgt tttccacctt
    19801 cacttacctg cggccctttc tcgaaatacg cacgggtgtg gggctcagtc agctgtcttt
    19861 cttgctgctt ggtctgggcc tggccggatt cgtcggtacc tacggggcca gcgcactgct
    19921 ggggcgacat ttgtatcgac tgctggcctt gctgccactg gcactggcgg ccacgaccct
    19981 ggcgtttctg ctgttgggtc atctgctctg gccggctgcg ttgttgttga ttgtctgggg
    20041 cgcgctcaat gcagcaatcc ccgtggcgtg gtccacctgg ctggcgcaag gcatcgccga
    20101 tgagcctgac gccggcggtg gtttactggt ggcggcaatc caactcgcga tcatgctggg
    20161 tgctgggttg ggtggggtgc ttctcgatca tttttcgctc agcgcaacct ggttgggtgg
    20221 ggctgtaatg ttgctgcttg gcgcactggt tgtgggcagc ggtcgccgct tacggcctgg
    20281 gtttgccacg gcttgaaagg ccacgcctgc cggcgctttg gccgcgccat taaatatttt
    20341 tccccgcacc tgtcgattca caattctgcc gttcgactaa cgtacagaac ccagccttgg
    20401 gtttgcggtc tgccattaca tacccagata aacccgtggc ggcaggctga tcgatcaact
    20461 acgcgaggac agcggcatgc gttttatggt gatagtcaag gcaactgcag attccgaagc
    20521 cggctttatg cccagcgagg agctgctgac ggcgatgggg cgttataacg aagagctggt
    20581 cgccgccggc gtgatgcagg caggtgaggg cctgcagcct agtagcaagg gcgcacgggt
    20641 gaagttctcc ggcagccagc gcagcgttac cgatgggccg tttatcgaga ccaaagagct
    20701 gatcgccggc ttctggattt tccaggtggc gtctctggcg gagtgcatcg agtgggtcaa
    20761 gcgttgcccc aacccgatgc ccggcgatag cgaaatcgag atccgacaaa ttttcgaggc
    20821 cgaggatttc ggcgacgaat tcacccccga gttgcgtgag caggaagaac gcctgcgcgc
    20881 gcggcttgaa tcgtaaaacc cccgcacgaa caaacgagta ctaaggagag tgcagatgaa
    20941 gatccacacc tatttaacct ttgacggcca atgtgaggct gctttcacgt tctacgccga
    21001 ctgcctgggc ggcgagttgg agttaatgcg catgggtgat agcccggaag cgggcaattt
    21061 gccggaggag tatcacaacc gtgtgatgca cgtttgcctg agcgtgggtg atcaactgct
    21121 gatggcgtcc gacagctgtc ctggcatggg gctaccgtac gagggcatca aaggctgttc
    21181 ggtttctctg caggtggaga acgtaccgga ggccgagcgt ctgtataaag cgctgtcggc
    21241 gggcggttcg gtgcagatgg agttgcaggc gaccttctgg gcgacccgct ttgccatgtt
    21301 caccgaccgc tttggcgtcg gctggatgat caattgcgat attgatgcgc agatgggtta
    21361 ggtatgcagg ctcggcatgg cgcagctgat ctatgcagct gcgccgttgc cgcctagcca
    21421 accagcccga gggcacggtc cagttgatcc tgcgggtcgc tgacggccgc gaggaattca
    21481 tcgccccagc ggcggatatc gttgtagtta acgatatcga acagctcacg caaccgcgcc
    21541 tgggcctcgg ccttgggcat gttcagcgcc acataacagg tttgcgtcat atccaccggg
    21601 tcatgcgggt tggttagcag cgcgcccttg agttcggcgg cggcgccggc aaactcggac
    21661 aacaccagca caccacgccc gtccagcaag ccttgcacgg cgacaaattc cttggccacc
    21721 aggttgaggc cgtcgcgcag tggggtaatc cacatcacgt cggccatggc ataccaggcg
    21781 ctgacttcct cgaatggcag gctgcggaag aagaactgca gcggcgtcca gccgatgcgg
    21841 gcgaagcggc cattgatgcg gcctacggcc tgctcgatct gcccttgcag ctcgtcatag
    21901 acggtcattt ccttggccgc tggcacgcag atggtcacca gggtgatctt gcccaacagc
    21961 tcggtgtttt cctccagcag gcgctcgtag gcattgagtt tttccagaat gcctttggtg
    22021 tagtcgaggc gttccaccga gaggatcagt ttgactccgg aaagctcggt gcgcagacgc
    22081 gccatcatct ccctgatttt tggcgcgaac agggcgttgc gcacccgttc gatatccaga
    22141 cccaccgggt gggcaccgag cttgactacg cggttgccgg tgtccactgc agtggtcatg
    22201 cgctccaggc caacggcgca gccataggtg ataaagcgtg gtgcgcagtt ctgtcggctg
    22261 acggtctgca gtggcatcac gccgcgtgcg gcatcgacga agttttccac ctggcgcggg
    22321 atatggaagc caatgtagtc gcactgcagc aggctgccga tgatctgccg gcgccagggc
    22381 agtacgttga acacatccgc cgaagggaag taggtgtggt ggaagaaggc gatgcgcagg
    22441 tccgggcgca gctcgcgcag gtagccgggg accatccaca ggttgtaatc gtgcagccag
    22501 actgtggcgc cttcagcggc ttccagagcg gtgcgttcgg caaaggcgcg gttgaccttg
    22561 aggaacacct gccagtcgtc ctcacggaac tgcgcacgct cccagaaggt gtgcagggtc
    22621 ggccagaagg cttccttgga gaagcgcttg tagaagatgt cgacctcttc cttgctcagc
    22681 gccacccgtg cggcggtcag cctcgggtag cgttcggcgt ccaccgtggt gtggctgtcg
    22741 aaggtttcgc cgttgttttc gtcatgctcg gcccaggcca cccaggagcc ggcgcgacca
    22801 tcgccgaaga agctcagcag ggtcgggata atgccgttgg gtgaagtcgg gcggcggcgt
    22861 tgcagcttgc catcggcgcc ccggaattct tcatagggca ggcggtgata caccattacc
    22921 agatcggcct taccggggtc ggcaacttgc ttgccttcgg cagcgatgcc ctgcttgccg
    22981 agaaagccaa agtgcacaaa ggcctcaaga atgccgccgc aaccggcgcg ctcggcgtgg
    23041 aagacccgcg aatactgccg ggtggcctgc agcaggccat cttcggacgc gcctacacac
    23101 acgcccttga actggccgct aagcatgctc agatcattca gggtgtcgcc ggcagtcagc
    23161 acctggtcgt tgtccagttc caaccattcg atcagcgcct gcaggctgct gcccttgtta
    23221 acgcccttgg gtagaaagtc caggtagcgc tcggccgaat aaagcagatc gcagcccaaa
    23281 ctctctgcga cctcctgcag ttccggatta gctgcctgct cggggctgca gaagtaggag
    23341 cagcggcgca cctgcggtac gtcctggcgc tccaagttaa agtgctcaat ggcgctggca
    23401 acctggctgt caccgggcca gagggcgtct actgcgcttt gcagcggctg gatcggctgc
    23461 aggctgtcgc catgcaccag gctagcgccg acgtctgaga tgatgaaatc cggttgcggc
    23521 agggtcgggt ctgccagcag gggcaaaact gactccaggc tgcggcccgt gacataggcc
    23581 aggcgtattt ccgggtgggc ggcgatggtt tgatagaggc ttagacgatc cttgggatcg
    23641 ccgccgagaa aggttccatc gagatcggtg gcaagtaaca tgcgctgtct tcctgtccag
    23701 ttaagtgggt gcagtgacgc gccgacaaaa gccgacgagc tatggacgat tgcgccagaa
    23761 ggcgttcgtc tcttggttta cccaggcgtc tgcggcgccg ggttaagcgg gtcctcagtt
    23821 gagtctgggg aatctgttgg gggggcatcg ggcatcagct cgagcaccgt gtggctggta
    23881 cgcagcatgg gcataaagtg cccggcgggt tcgctgacca ggtcgctgac atggcgcaga
    23941 cggtacagcg tgtacgccgc cagcaaaccg agcgtggcgg cgaaatacag cggcagcgcc
    24001 tgggcgccga ggtgctgcat cagcaggcca gcgagcagtg ggccgcacac cgagccgatg
    24061 ccgttgacca tcaacaggct gctggagcca gagagaattt catcgctgtg cagttggtca
    24121 atcaactgcg ctacggcgat ggggtagatg gcaaaggcca agccgcccca gataaacatc
    24181 agcccgagca gcaaacgacc ggccggcagc aggctcatta gcacggcaac cactacggcc
    24241 agcgccacta cccagaacag caccaggcga cgatcgtgtt tatccgaata gatgccaatc
    24301 ggccattgca gcaaggcgcc gccgagaatc gtgctgctca tcaacaaacc tacgccagtg
    24361 gcgtcgaaac cgttcagggc cgcgtacacc ggggccatac cccagaacgc gccgagggcc
    24421 aggcccgata aaccagcggc ggccaacgcc agtggggcga taccggcgat ctgccgcaga
    24481 ttggtgtgca gggtatccgg cacgctgggt tggatctgcc gagtcagggt aatgggcatc
    24541 aaggccgcgc tgatcaacat tgcggccagc acgaacaata cgaagtcact tggcgacgcc
    24601 agattgagca gctgctgcgc ggcggccagc gcgccgaggt tgactgccat atacacggca
    24661 aacacctggc cgcgtttgtc gttgggcacc tgcgcgttaa gccagctctc gatcaccata
    24721 tacaggctga ccagcgccag gccgtaaagc a
//
LOCUS       DASRUM010000235         6057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2137896, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000235 DASRUM010000000
VERSION     DASRUM010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2137896"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VFF22_15745"
     CDS             complement(<1..1043)
                     /locus_tag="VFF22_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011103546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZX18304.1"
                     /translation="MALLQIAEPGQSPQPHQRRLAVGIDLGTTNSLVAAVRSGLSEPL
                     ADAAGQVILPSAVRYHADRVEVGEVAKAAASADPLNTVLSVKRLMGRGLADVKQLGEQ
                     LPYRFVGGESHMPFIETVQGPKSPVEVSADILKVLRQRAEEALGGELVGAVITVPAYF
                     DDSQRQATKDAARLAGLSVLRLLNEPTAAAVAYGLDQHAEGVVAIYDLGGGTFDISVL
                     RLTGGVFEVLATGGDSALGGDDFDHAIAGWIVEQAGISSDLDPAQQRLLLEAACAAKE
                     ALTDSATASLSYAGWVGQLSREQFDALIEPMLARSLKACRRAVRDAGIELDEVEAVVM
                     VGGSTRVPRVRQAV"
     gene            complement(1092..1613)
                     /gene="hscB"
                     /locus_tag="VFF22_15750"
     CDS             complement(1092..1613)
                     /gene="hscB"
                     /locus_tag="VFF22_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HZX18305.1"
                     /translation="MGSPCHFALFDLQPSFRLDLDQLAARYRELARNVHPDRFADASE
                     RDQRLALEQSASLNEAYSTLKSAPQRARYLLALRGPELPLEVTVQDPDFLLQQMQLRE
                     ELEDLHDSADLAGVAQFKRRLKVAQDELNESFAVCWDDASRREEAERLMRRMQFLDKL
                     SHEVRQLEERLDD"
     gene            complement(1621..1944)
                     /gene="iscA"
                     /locus_tag="VFF22_15755"
     CDS             complement(1621..1944)
                     /gene="iscA"
                     /locus_tag="VFF22_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HZX18306.1"
                     /translation="MAISMTEAAAQHVRRSLEGRGKGEGIRLGVRTTGCSGLAYVLEF
                     VDEAASEDQVFENHGVKVIIDPKSLVYLDGTELDFVKEGLNEGFKFNNPNVRGECGCG
                     ESFNI"
     gene            complement(1957..2343)
                     /gene="iscU"
                     /locus_tag="VFF22_15760"
     CDS             complement(1957..2343)
                     /gene="iscU"
                     /locus_tag="VFF22_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HZX18307.1"
                     /translation="MAYSEKVIDHYENPRNVGKMDAEDPDVGTGMVGAPACGDVMRLQ
                     IKVNEQGVIEDAKFKTYGCGSAIASSSLATEWMKGKTLDEAETIKNTQLAEELALPPV
                     KIHCSVLAEDAIKAAVRDYKQKKGLL"
     gene            complement(2391..3605)
                     /locus_tag="VFF22_15765"
     CDS             complement(2391..3605)
                     /locus_tag="VFF22_15765"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HZX18308.1"
                     /translation="MKLPIYLDYSATTPVDPRVAQKMSECLLVDGNFGNPASRSHVFG
                     WKAEEAVENARRQVAELVNADPREIVWTSGATESNNLAIKGIADFYKTKGKHLITSKI
                     EHKAVLDTMRQLEREGFEVTYIEPGEDGLITPAMIEAVLREDTILVSIMHVNNEIGTI
                     NDIAAIGELTRSRGVFFHVDAAQSTGKVAIDLDAFKVDLMSFSAHKTYGPKGVGALYV
                     RRKPRVRLEAQTHGGGHERGMRSGTLATHQCVGMGEAFRIAKEEMTQENQRILALRER
                     FFKQVDGLEELYINGSMSARVPHNLNLSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSIRFTFGRFTSDEEVDYAAQKVCEAVTKLRELSPLWDMYKD
                     GVDISKIEWAAH"
     gene            complement(3654..4145)
                     /gene="iscR"
                     /locus_tag="VFF22_15770"
     CDS             complement(3654..4145)
                     /gene="iscR"
                     /locus_tag="VFF22_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HZX18309.1"
                     /translation="MRLTTKGRYAVTAMLDLALHAQHGPVSLADISERQGISLSYLEQ
                     LFAKLRRGNLVSSVRGPGGGYQLSRDMQGIQVAEVIDAVNESVDATRCQGQGDCHAGD
                     TCLTHHLWCDLSQQIHEFLSGISLADLVTRREVQEVAQRQDQRRCNGRTPYLDKIETS
                     AVD"
     gene            complement(4285..5061)
                     /gene="cysE"
                     /locus_tag="VFF22_15775"
     CDS             complement(4285..5061)
                     /gene="cysE"
                     /locus_tag="VFF22_15775"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HZX18310.1"
                     /translation="MFERMREDIQSVFHRDPAARNAFEVVTCYPGLHAVWLHRLAHAL
                     WGSGWKWLARVVSNFGRWTTGIEIHPGAKIGRRFFIDHGMGIVIGETAEIGNDVTLYQ
                     GVTLGGTSWNKGKRHPTLEDGVVVGAGAKVLGPFTVGAGAKIGSNAVVTKEVPAGATA
                     VGIPGRIIVKTDDEQEAKRQAIAEKLGFDAYGVSQDMPDPVARAIGQLLDHLHAVDGR
                     LEGMCGALKSLGSDYCAKDLPQLRDEDFAGVVKDADKPAA"
     gene            complement(5054..5824)
                     /gene="trmJ"
                     /locus_tag="VFF22_15780"
     CDS             complement(5054..5824)
                     /gene="trmJ"
                     /locus_tag="VFF22_15780"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HZX18311.1"
                     /translation="MLENIRVVLVGTSHPGNIGGAARAMKNMGLSQLVLVDPRDFPSE
                     DAVARASGATDILDAAQVVGTLEEALVGCRLVLGTSARDRHIPWPMLEPRECGVAACE
                     QAQQGAQVALVFGREYAGLTNEELQRCHFHVHIPSDPAFSSLNLAAAVQVLAYEVRMA
                     WLATEGQPLKQVAVVDPDEEPVAADELESFYRHLEQALVEIRFLDPAKPRHLMSRLRR
                     LYGRSAVSRLEMNILRGILTETIKAARGEHPRQEKADV"
     gene            5969..>6057
                     /locus_tag="VFF22_15785"
     CDS             5969..>6057
                     /locus_tag="VFF22_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HZX18312.1"
                     /translation="MQPMLNIALRAARSAGEMIFRSTERLDVIS"
BASE COUNT         1275 a         1973 c         1633 g         1176 t
ORIGIN      
        1 acggcctgac gcacgcgcgg cacgcgggtg gagccgccaa ccataaccac ggcttcaacc
       61 tcatccaact caatgcctgc atcacgcacc gcgcggcggc aggccttgag gctgcgcgcc
      121 agcatcggct cgatcagcgc atcgaactgc tcacgactca attgccctac ccagccggca
      181 tagctgaggc ttgccgtagc gctgtcggtc agcgcttctt tggcggcgca ggcggcctcc
      241 agcaatagac gctgctgtgc tggatcgaga tcagaggaaa tccctgcctg ctcgactatc
      301 caaccagcaa tcgcgtgatc gaagtcatca ccgcccagcg cgctgtcacc accggtagcc
      361 agcacttcaa acacgccgcc agtcaggcgc agaacggaaa tatcgaaggt accgccgccc
      421 aggtcataaa tggcgaccac gccctcggcg tgctgatcca gaccataagc cacggcagca
      481 gcagtcggct cattgagcag acgcaacaca ctcaagccgg ctaggcgtgc agcatctttg
      541 gtggcctgac gctgcgaatc gtcgaaatag gccggcacgg tgattaccgc accgaccagc
      601 tcaccaccga gggcctcctc ggcgcgctga cgcagcacct tgagaatatc cgccgagacc
      661 tcgaccgggc ttttcggccc ctgcacggtt tcgataaacg gcatatgcga ttcgccgcca
      721 acaaagcggt acggcagctg ctcgccgagc tgcttgacgt ccgccagacc acggcccatt
      781 agacgcttga ccgacagcac ggtattcagc ggatcagccg atgcggccgc cttggcgact
      841 tcgcccacct caacacggtc ggcgtgatag cgcaccgcgg acggcagaat aacctgccca
      901 gcggcatcgg ccagcggctc ggaaaggccg ctacgcacgg cggcgaccag cgaattggtg
      961 gtacccagat caatccccac cgccagtcga cgctggtgtg gctgggggct ttgtccgggc
     1021 tcagcgatct gcagtaaggc catgattaat cagtgcttag atatcaggcg cgccgcatag
     1081 gcacgccagc gttaatcgtc gaggcgctct tccagctggc ggacctcgtg agagagcttg
     1141 tcgagaaact gcatgcgacg catcaggcgc tcggcctctt cgcggcgcga agcatcatcc
     1201 caacaaacgg caaaactttc gttgagctca tcctgagcca ctttcagacg ccgcttgaac
     1261 tgcgcaacgc ccgccaggtc tgcgctgtcg tgcaggtctt ccagctcttc gcgcaattgc
     1321 atctgctgca gcaggaaatc cggatcctgc accgtcactt ccagcggcag ctcagggccg
     1381 cgcaatgcca gcaaatagcg ggcacgctgc ggcgcactct taagcgtact gtaagcctcg
     1441 ttgagactgg cggactgctc gagggccagg cgctgatcac gctctgacgc atcggcgaag
     1501 cggtcaggat ggacattgcg cgccagctcg cgataacgcg cggctagctg gtcaagatcc
     1561 aggcgaaagc tcggctgcag gtcaaataaa gcaaagtgac aaggactgcc cacacgcgcc
     1621 tcagatattg aagctttcgc cgcagccaca ttcgccgcgt acgttcgggt tgttgaactt
     1681 gaagccttcg ttcaaccctt ctttgacgaa gtcgagctcg gtgccatcga ggtagaccag
     1741 gcttttggga tcaatgatga ccttgacgcc atggttctcg aacacctgat cttcgctggc
     1801 ggcctcatcg acaaactcca gcacataggc caagccggaa cagcccgtgg tgcgcacacc
     1861 caggcgaata ccttcgccct tgccacgccc ttcaagcgag cgacgtacgt gctgagcggc
     1921 agcttcggtc atgctgatag ccatcgacaa acctccttag agcaagcctt tcttctgctt
     1981 gtaatcgcgt acagcggcct tgatggcatc ttcggcgagt accgagcagt gaattttcac
     2041 cggtggcaga gccaattcct cggccagctg ggtgttcttg atggtttctg cttcatccag
     2101 ggtcttgccc ttcatccact cggtagcgag ggagctggag gcaatcgccg aaccgcaacc
     2161 gtaggtcttg aacttggcgt cttcaatcac gccctgctcg ttgaccttga tctgcagacg
     2221 catcacgtca ccgcaagccg gcgcgccgac catgccggtg ccgacatctg ggtcttccgc
     2281 gtccatcttg ccgacgttgc gcgggttttc gtaatggtca atgacctttt cactgtaagc
     2341 catgttggta ttccttcctc atcagagagc cgctcttgct tggcgactac ttagtgcgcc
     2401 gcccactcaa tcttggagat gtcgacaccg tctttgtaca tatcccacag cggcgacaat
     2461 tcgcgcagct tggtgaccgc ctcgcacacc ttctgcgcgg cgtaatcgac ttcctcatca
     2521 gaggtgaagc ggccgaaggt aaagcgaatg gagctgtgtg ccagttcgtc attgcggccc
     2581 agggcgcgca atacgtagga cggctccagg gaagcagagg tacaggccga gccggacgaa
     2641 accgccaggt ccttgagcgc cataatcagc gactcaccct caacgtagtt aaaactgagg
     2701 ttgaggttgt gcggtacgcg ggcgctcatg ctgccattga tgtacagctc ttccaggcca
     2761 tcgacctgct tgaagaagcg ctcacgcagg gccaggatgc gctggttttc ctgggtcatt
     2821 tcttccttgg caatgcggaa cgcttcgccc atgccgacgc actggtgggt cgccagggtg
     2881 ccggaacgca tgccgcgctc atggccgcca ccatgggtct gggcttccag acgcacacgc
     2941 ggcttacgcc gtacgtacaa cgcaccaacg ccttttggac catatgtttt gtgcgcggag
     3001 aaggacatca gatcgacctt gaacgcgtcg agatcaattg ccaccttgcc ggtggactgc
     3061 gccgcatcaa catggaagaa cacgccgcgc gagcgggtca gctcaccgat tgcagcgatg
     3121 tcgttgatgg tgccgatttc attattcacg tgcatgatcg acaccagaat agtgtcctca
     3181 cgcagcactg cctcgatcat ggccggggta atcaggccat cttcgccagg ctcgatgtag
     3241 gtcacttcga aaccttcgcg ctcaagctga cgcatggtat ccagcaccgc tttgtgctcg
     3301 atcttcgaag tgatcaggtg cttgccttta gtcttgtaaa agtcggcaat gcccttgatc
     3361 gccaggttgt tcgactcggt cgcaccggaa gtccagacaa tctcacgcgg gtcggcatta
     3421 accagctcag ccacctggcg gcgagcattt tctacagctt cttcagcctt ccagccgaac
     3481 acgtgggagc gcgacgccgg attgccgaag ttgccgtcga ccagcaggca ttcgctcatt
     3541 ttttgcgcaa cacgcggatc aaccggcgtg gtggcggaat aatcgaggta aatcggcaac
     3601 ttcatcaatt ctctcctaat caggtatcag gccagctagc cagcgctttg cgctcaatcg
     3661 acggcagacg tttcgatctt atccaggtac ggcgtcctgc cattgcagcg gcgctgatcc
     3721 tggcgctggg caacttcctg cacttcacgg cgggtcacca agtcagcgag actgatgcca
     3781 cttagaaatt cgtgtatctg ctggctgagg tcacaccaca aatgatgggt cagacaggta
     3841 tcccccgcgt ggcaatcgcc ctgcccctgg cagcgcgtgg catcgaccga ttcgttgacc
     3901 gcatcgatca cctcagccac ctggataccc tgcatgtcgc gcgacagctg ataaccaccg
     3961 cccggcccac gcacactgga aaccagatta ccgcgacgca acttggcaaa gagctgttcc
     4021 agataggaaa gggaaatacc ctgccgctcg gaaatatcag ccagagacac cggcccatgt
     4081 tgcgcgtgca acgccagatc aagcatggcg gtaacggcgt aacggccttt ggtggttagt
     4141 cgcataaggg attaccgcgt ggtcgcaata tgccgcgagt atgcaattcc cgagcattta
     4201 agtcaactat aagacctact gctttagtcg ggattagcca aaatggaggg cgcgcagcat
     4261 agcaaaatgc gcgcgccagc accatcaagc cgcaggcttg tctgcgtctt tgaccacgcc
     4321 cgcgaagtcc tcgtcacgca gttgcggcaa atccttggcg cagtagtcac tgcccaggga
     4381 cttgagcgcg ccgcacatac cttccagccg cccatccacc gcatgcaggt gatcgagcag
     4441 ctgaccaatg gcgcgcgcta ccgggtccgg catgtcctga ctcacgccat aggcatcgaa
     4501 accgagcttc tcggcaatag cctggcgctt ggcttcctgc tcatcgtcag ttttgacgat
     4561 gatccgcccc ggtatgccaa ccgccgtagc gcccgccggc acctccttgg tcaccacggc
     4621 attagagccg atcttggcgc ccgcgccgac cgtgaaaggc cccagcacct tggcaccagc
     4681 tccgaccacc acaccatctt ccagcgtcgg atggcgctta cccttgttcc aactggtgcc
     4741 gccaagggtt acaccctggt aaagagtgac gtcattgccg atctcggcgg tttcaccgat
     4801 gacgatgccc ataccgtggt cgataaagaa acgccggcca atcttcgcac ccgggtgaat
     4861 ttcgataccc gtcgtccagc gcccgaagtt cgacaccact cgcgccagcc acttccagcc
     4921 actgccccac agcgcatgcg ccaagcgatg cagccacacc gcatgcaacc ccggatagca
     4981 agtgaccacc tcaaaggcat tacgcgccgc cgggtcgcga tgaaatacgc tttggatatc
     5041 ctctcgcatg cgctcaaaca tctgccttct cctgcctagg gtgctcgcca cgcgccgcct
     5101 tgatggtttc cgtcaagata ccgcgcaaga tattcatttc cagcctgctc accgcactgc
     5161 gcccatacaa gcggcgcaag cgactcatca aatgccgcgg cttggccgga tcaagaaacc
     5221 tgatctcaac cagcgcctgc tccagatgac ggtaaaacga ttccaactca tccgccgcaa
     5281 ccggctcttc atccggatcc actactgcaa cctgcttgag cggttgcccc tccgtcgcca
     5341 gccaggccat acgcacttcg taagccagta cctgcaccgc cgccgccaga ttcaacgagc
     5401 tgaacgctgg atcagaggga atatgtacat gaaagtgaca gcgctgcagc tcctcattgg
     5461 tcagaccagc atattcacga ccgaacacca aggccacctg cgcgccttgc tgcgcttgtt
     5521 cacacgccgc tacgccacac tcgcgcggct ccagcatcgg ccagggaatg tggcgatccc
     5581 gcgcactggt gcccagcacc aggcgacaac cgaccagcgc ctcttcaagc gtgccgacca
     5641 cctgcgccgc atcgagaata tcagtagccc ccgaggctct ggccaccgcg tcttcacttg
     5701 ggaaatcccg cggatcaacc agcaccagct gcgacaaacc catgtttttc atggcccgcg
     5761 ccgcgccacc gatattgcct ggatgactgg tgccgaccaa cacaacgcga atattttcca
     5821 gcaacgcaaa cgctcacaga taaggcttag aggggcaaat cttacagaag ccacccgcct
     5881 tgcgccacgc aaggcgcagg ttgcacttca acctctgggc ttttctgcta gaatggccgg
     5941 ctttctttaa cgacccaggt gaacatccat gcagcccatg ctgaatatcg cgctgcgcgc
     6001 agcccgtagc gccggcgaaa tgatcttccg ctcgaccgaa cgcttggacg tcatctc
//
LOCUS       DASRUM010000236         2913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2137956, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000236 DASRUM010000000
VERSION     DASRUM010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2137956"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="VFF22_15790"
     CDS             complement(<1..1020)
                     /locus_tag="VFF22_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZX18313.1"
                     /translation="MIENLRNIAIIAHVDHGKTTLVDKLLRQSGTLERNELNDERVMD
                     SNDQEKERGITILAKNTAINWNGYHINIVDTPGHADFGGEVERVMSMVDSVLLLVDAQ
                     DGPMPQTRFVTKKAFEAGLRPIVVINKVDRPGARPDWVLDQIFDLFDNLGATEEQLDF
                     KVVYASALNGIAGLDHTDMAEDMTPLYQSIVDNVPSPQVDIDGPFQMQISALDYNSFL
                     GIIGVGRIARGRVKPNTPVIAIDADGKKRNGRILKLMGHHGLHRIDVEEATAGDIVCV
                     SGMDSLFISDTLCDINHVEAMTPLTVDEPTVSMTFQVNDSPFCGKEGKFVTSRNIKER
                     LDKELL"
     gene            complement(1182..2636)
                     /gene="thiI"
                     /locus_tag="VFF22_15795"
     CDS             complement(1182..2636)
                     /gene="thiI"
                     /locus_tag="VFF22_15795"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HZX18314.1"
                     /translation="MKLIVKVFPEITIKSRPVRKHFIRQLAKNIRSVLREFDPQLQVS
                     GVWDNLEVETQISEPKTLQAMIERLSCTPGITHFLQVDEYPLGDFDDVLEKCKLHFAD
                     QLPGKIFAVRCKRAGKHTFSSMDVERYVGSQLRQQCGAAGISLKDPDVVVRMEIRDQR
                     LFVIHHQHEGLGGYPLGSLEQTLVLMSGGFDSTVAAYQMMRRGLVTHFCFFNLGGRAH
                     ELGVMEVAHYLWQKFGSSQRVLFISVPFEEVLGEILGKVDNSQMGVILKRMMLRASTR
                     MAERLQIDALVTGEAISQVSSQTLPNLAVIDSATDMLVLRPLLASHKQDIIDTATRIG
                     TAEFAKHMPEYCGVISVNPTTKAKRPRIEYEESQFDMAILERALERATLLPIDKVIDE
                     LGKDVQVEEVSEALAGHVIIDIRHPDSAEDQPLELPGIEVQALPFYALNSKFKELDEA
                     RQYLLYCDKGVMSRLHAHHLLSEGHANVRVYRPA"
BASE COUNT          577 a          835 c          874 g          627 t
ORIGIN      
        1 cagcagctct ttgtccagac gctctttgat gttgcggctg gtaacgaact tgccttcttt
       61 accgcagaac ggcgaatcgt tgacctggaa ggtcatggat acggtcggct cgtcgacggt
      121 cagcggggtc atcgcttcaa cgtggttgat gtcgcacagg gtgtcggaga tgaacagcga
      181 gtccatgccg ctgacgcaga cgatgtcacc cgcggtggct tcttcaacgt cgatgcggtg
      241 caggccgtgg tgacccatca gcttgaggat acgaccgtta cgcttcttgc cgtcggcgtc
      301 gatggcgatc accggggtgt tcggcttgac gcgaccacga gcgatacggc caacgccgat
      361 aatgccgagg aagctgttgt agtccagtgc ggagatctgc atctggaacg gaccgtcgat
      421 atcaacctgc ggggacggca cgttgtcgac gatggactgg tacagcgggg tcatgtcttc
      481 agccatgtcg gtgtgatcca gaccggcaat gccgttcagg gccgaggcgt agacgacttt
      541 gaagtccagc tgctcttcgg tagcaccgag gttgtcgaac aggtcgaata tctggtccag
      601 aacccaatcc ggacgcgcgc ctggacggtc aaccttgttg attaccacga ttggacgcag
      661 gccggcttcg aaggccttct tggtcacgaa acgggtttgc ggcatcgggc cgtcttgggc
      721 gtcgaccagc agcagtacgg agtcaaccat cgacatcacg cgctcaactt caccgccgaa
      781 gtcggcgtgg ccgggggtgt cgacgatgtt gatgtggtag ccgttccagt tgatcgcggt
      841 gttcttcgcg agaatggtaa tgccgcgctc tttttcctgg tcgttgctgt ccatcacgcg
      901 ctcgtcgttg agctcgttgc gctcaagggt gccggactga cgcaggagtt tgtctaccag
      961 ggtggtctta ccatggtcaa cgtgggcaat gatggcgatg ttgcgtagat tttcgatcac
     1021 gtgtgtatct cgatcagagg attcggtgtg ccgcttattc taggcggcga gtattaacta
     1081 attgcgattt ctgccaggcg gcgtacgagc gctgtggctg ttacgccgcc tgtgggtcgg
     1141 gagggcgatg gcggatactg ccgccattca acccgggcgt tttatgccgg acgataaacg
     1201 cgcacattgg catgcccctc actcagcaag tggtgggcgt gcaaacgact catcacacct
     1261 ttatcgcaat acaacaggta ctggcgcgcc tcatccagct ccttgaactt gctgttcaag
     1321 gcatagaacg gcagcgcttg cacttcgatg ccgggcagct caaggggctg atcttcggcg
     1381 ctatcggggt ggcggatgtc gatgatgaca tgaccggcca gggcttcgct gacttcctcg
     1441 acctgcacgt ccttgcccag ctcgtcgatc actttgtcga tcggcagcaa ggtcgcgcgt
     1501 tccagcgcgc gctcgaggat ggccatgtcg aactggcttt cctcgtattc gatacgcggc
     1561 ctcttcgcct tggtggtcgg gttgaccgaa atcaccccgc aatattccgg catatgtttg
     1621 gcgaactcgg cagtgccgat gcgcgtggcg gtatcgatga tgtcctgctt atgactggca
     1681 agcagcggac gcagcaccag catgtcggtt gcggaatcga tcaccgcgag gttcggcagg
     1741 gtctggctcg atacctggga aatcgcctcg ccagtgacca gcgcatcaat ctgcagacgc
     1801 tcggccatgc gcgtggaggc gcgcagcatc atgcgcttga ggatcacacc catctggctg
     1861 ttgtcgacct tgccgagaat ctcgcccagc acttcctcaa acggcacgct gataaacaac
     1921 acgcgctgcg agctgccgaa cttctgccag aggtaatgcg ccacttccat cacgcccagc
     1981 tcgtgggcac ggccgccgag attaaagaag cagaagtggg tgaccaggcc gcgacgcatc
     2041 atctggtaag cggcgacggt ggaatcgaaa ccgccgctca tcagcaccag ggtctgctcc
     2101 agcgagccga gcggataacc gcccagtcct tcgtgctggt ggtgaatcac gaacaggcgc
     2161 tggtcacgga tctccatgcg caccacgacg tcgggatctt tcagcgagat gccggccgcg
     2221 ccgcactgct ggcgcaactg gctgccaaca tagcgctcga catccatgga gctgaatgtg
     2281 tgcttgccgg cgcgcttgca gcgcacggcg aagatcttgc ccggcagctg atcggcgaag
     2341 tgcagcttgc acttttccag cacgtcgtcg aaatccccca gcgggtattc atcaacttgc
     2401 aggaaatggg tgatgccagg cgtgcagctc aagcgctcga tcatcgcctg cagggttttc
     2461 ggctcactga tctgggtttc gacttccagg ttgtcccaca caccgctgac ctgcagctgt
     2521 ggatcgaact cacgcaaaac cgaacgaatg ttcttcgcca gctgccggat aaagtgctta
     2581 cgcaccggcc gacttttaat ggtgatttct gggaagactt taacgatcag tttcatgaaa
     2641 acagtgagtg ccgaggccgc gaaaaaacag gggcgcggat tatagcggaa attgctcacg
     2701 ctttgatcaa aaatcctcac acgctcaaaa cacgcaccat ttaagggcgg acaacaaata
     2761 tggcgaccca taacggtgca aatattttcc aggcagcgcc gcgcagcccc cgccagccct
     2821 gagtttcagg gcttacaccc attcctgtgc actggcatgc aatttgctcc cttgtgaggc
     2881 aggatgccct ggcggagtat gccgcggccg tac
//
LOCUS       DASRUM010000237        14863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_956302, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000237 DASRUM010000000
VERSION     DASRUM010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 14863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14863
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956302"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFF22_15800"
     CDS             <1..225
                     /locus_tag="VFF22_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18315.1"
                     /translation="RLGHDTLSGTLCYMFAIGFKHSYYPHQWSQRVVRDAIQKTTNQA
                     LRGFMGRSAEVLLLSCRSCFQSFTPAGRAG"
     gene            469..945
                     /locus_tag="VFF22_15805"
     CDS             469..945
                     /locus_tag="VFF22_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HZX18316.1"
                     /translation="MSNQRIKTPCVGLCSTVYGDLVCRGCKRFHHEVINWNGYNDDEK
                     RAVWLRLEVLLVQVMAAKLEIFDADRLRMQLEQRKIRFVPQQSPYCWAYQLIARGARV
                     ISQLNAYGMVLLPEFREWNLPELRDAIDREFFLLSEAHYERYIAPRFLLEGLEIRV"
     gene            complement(1007..1870)
                     /locus_tag="VFF22_15810"
     CDS             complement(1007..1870)
                     /locus_tag="VFF22_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZX18317.1"
                     /translation="MQAIRSILVVMEPNHTEDLALKRAKLIAGVTQSHLHLLVCDKKS
                     DHTGYLRDLTGLLNSEGFSTSSQQAWDENQHQTIINVQQAEGCGLVIKQHFPDNPLKK
                     ALLTPDDWKLLRYCPAPVLMVKTEKSWAGTAILAAVDVGNADSEHRTLHTSIVSHGFD
                     IAALAKAELHVISAHPAPMLSAADPTFQLKETIEARYREQCKAFQAEYEISNERLHIA
                     EGPADVLIPQTAHRLNAGVTVIGTVARTGISGALIGNTAEVILDSLESDILVLKPDDI
                     IAHLEELVAQR"
     gene            2120..2737
                     /gene="miaE"
                     /locus_tag="VFF22_15815"
     CDS             2120..2737
                     /gene="miaE"
                     /locus_tag="VFF22_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HZX18318.1"
                     /translation="MNLPEIHEFLGCRTPDAWITAALADQQTLLIDHKNCEFKAASTA
                     LSLMAKYSTYVDLLNAMSRLAREELVHHEQVLRIMKRRKIGLRPVSAARYASGLRKVV
                     RTHEPHKLVDTLVVGAFIEARSCERFEALVPHLDEELGKFYFGLLKSEARHFQNYLKL
                     AYQYGDERDVNQAIERVRVAEQELIESPDTEMRFHSGVPPLPLVI"
     gene            complement(2734..3453)
                     /gene="lpxH"
                     /locus_tag="VFF22_15820"
     CDS             complement(2734..3453)
                     /gene="lpxH"
                     /locus_tag="VFF22_15820"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003289615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HZX18319.1"
                     /translation="MILLISDLHLEQERPDISRAFLHFLETRAVQAQALYILGDFFQV
                     WIGDDAITPFQRSIAQALRQLSERGTRIYLMHGNRDFLLGKAFCREAGCTLLPDPCVI
                     ELNGERVLLMHGDSLCTQDIGYMKLRRWLRNPLSLFILRNLPLSTRQKLARKLRNESR
                     TQTRMKASEIVDVTPDEVPRIMAEHGVRTLIHGHTHRPATHQLQVNGQPAQRIVLGDW
                     DKQGWALQVDKQSFNQAPFTL"
     gene            complement(3450..3944)
                     /locus_tag="VFF22_15825"
     CDS             complement(3450..3944)
                     /locus_tag="VFF22_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZX18320.1"
                     /translation="MIKLHTNHGVITLNLFEDKAPETVANFKQYVQEGHYDNTVFHRV
                     ISNFMIQGGGFEPGMKQKTTRAPIKNEANNGVANKIGTVAMARTMEPHSASAQFFINV
                     GDNSFLNHSAPTVQGWGYAVFGEVAEGMDVVNAIKAVATTSKSGHQDVPVDDVIIERA
                     EIVE"
     gene            4108..5772
                     /locus_tag="VFF22_15830"
     CDS             4108..5772
                     /locus_tag="VFF22_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HZX18321.1"
                     /translation="MSKPETVAAANFLRPIVQADLDAGKHAKIITRFPPEPNGYLHIG
                     HAKSICLNFGLAKEFGGECNLRFDDTNPAKEDQEYIDAIKSDVQWLGFQWAGEERYAS
                     NYFDQLHAWAIELIKAGKAFVCDLNAEEMRAYRGNLTEPGKNSPFRERSVEENLDLFA
                     RMKAGEFADGARSLRAKIDMTSPNINLRDPILYRIRHAHHHQTGDKWCIYPSYDFTHG
                     QSDAIEGITHSICTLEFEDHRPLYEWFLAQLPVPAQPRQYEFARLNLNYTITSKRKLK
                     QLVDEKHVNGWDDPRMSTLSGFRRRGYTPASIRTFCDMIGVNRAGGVVDVGMLEFAIR
                     DDLDANAARAMCVLKPLKVVITNYPEGKVENLELARHPKQDMGVRVLPFSREIYIDAS
                     DFEEVPPAGFKRLIPGGEARLRGSYVIRADEAIKDAEGNIVELRCSYDENTLGKNPEG
                     RKVKGVIHWVPAAESVECEVRLYDRLFRSANPEKDEEGGSFLDNINPDSLVVLKGCRA
                     EPSLAEALPEERFQFEREGYFCADIKDSRPGAPVFNRTVTLRDSWA"
     gene            5835..7217
                     /gene="cysS"
                     /locus_tag="VFF22_15835"
     CDS             5835..7217
                     /gene="cysS"
                     /locus_tag="VFF22_15835"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HZX18322.1"
                     /translation="MLSIYNTLTKSKDVFKPLVGNQVRMYVCGMTVYDFCHIGHARVM
                     VAFDVVTRWLRHRGYEVTYVRNITDIDDKIIKRAQENGEPFEVLVERMIAAMHEDEAR
                     LNVLRPDQEPRATGHIAGMHEMIQTLIDKGFAYAADNGDVYYRVGKFVGYGKLSRRKI
                     EDLKIGARIEVDEAKQDPLDFVLWKAAKPGEPSWESPWGAGRPGWHIECSVMSTCCLG
                     ETFDIHGGGPDLVFPHHENEIAQSEAATGKLYANAWMHAGAVRVDGEKMSKSLGNFFT
                     IREVLEKYHPEVVRYLLVSSHYRSPINYSEDSLREAKGALERFYNALKGLPVAVPAGG
                     EAFVERFAAAMDDDFNSPEACAVLFELAREINRLRDSDLQAAAGLAARLKELAAVLGV
                     LQLEPEAFLQAGAAGKVDAAEVEALIAARLQARAEKNWGESDRIRDQLTAMGVVLEDS
                     KGATTWRLAD"
     gene            complement(7277..8107)
                     /locus_tag="VFF22_15840"
     CDS             complement(7277..8107)
                     /locus_tag="VFF22_15840"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017131014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZX18323.1"
                     /translation="MALLSACSSLAPPHPNPEVEPIRFLLSFDDGPSASTRDNPTEQI
                     LDTLADNPIQPGIKALFFVQTRAAGAGGSVQGQALLRRQQREGHLLGFHTATSGHSNH
                     RFLSPAALEQSLKDGVADLTAISGAAPRLVRPPFWNYDERTFAAYQKHGLRLLLTDLS
                     ANDGKTWGINGSLRRRSNLRDQLARAAQQISVGALPAVDGVVPIVVTFHDLNPYTARH
                     MQEYLQILLDVAEDVGLATDKQPFYGQRDALQRAALRRALGDPLQYARLPGLWNWLWN
                     "
     gene            complement(8379..10250)
                     /locus_tag="VFF22_15845"
     CDS             complement(8379..10250)
                     /locus_tag="VFF22_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HZX18324.1"
                     /translation="MADAVTALPHETIIQDSWTRCRDYGLTHQSTPIFSQPERGEVSA
                     LLESQHALVHTTHKEVLPYYGTILANSNCLIMLADHQGQVLQSWGDQRFIEPNRAAGF
                     VAGATWLERYTGTNAIGTALSCGQAVHIQHDEHFLKANRFMTGSASPIFDEQRQMIAV
                     LDVSSDSYLPPSHTLGMVKMMSQSVENRLILNLFQQRYFQLSFNTSLDNLSSPWAGLL
                     IFDEQGQVVSANRRADSLLGVGLAQVNIESLFDVPLQQLLNQPEALPFNLRASGRFRF
                     HAQLKRPQLPSPIQARDFRQAQQPPASAKHNPFTLASLNLGDPRVDKAVRQAERLLEK
                     NIPILVHGETGVGKEVFVKALHHASSRASQAFIAVNCAAIPAELVESELFGYEKGAFT
                     GANQKGSIGLIRKADKGTLFLDEVGDMPLRVQARLLRVLQERCVQPLGSSELYPVDIR
                     LISATNRPLRQDVESGLFRQDLYYRISGLNLELPPLRERTDKAAMVQRIWDYHREPQQ
                     WAGLSHEVLSLFERHPWPGNLRQLSSVLQIALAMADDQQIRPEHLPDDFFADLQPAAT
                     QAAATQQPPQPLASTSADDLASQYQACGGNISHLARSLGVSRNTLYKRLRETGLTVR"
     gene            10511..11605
                     /locus_tag="VFF22_15850"
     CDS             10511..11605
                     /locus_tag="VFF22_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX18325.1"
                     /translation="MSLTLEHVTRIVDHQLYIDDACLSFEPGSFNVLLGRTLSGKTSL
                     MRLMAGLDKPTSGRILMNGADMTNVSVRQRNVSMVYQQFINYPTLTVFENIASPLRQA
                     GVSKAEILEKVHATAKMLRIEALLQRHPLELSGGQQQRTAMARALVKDASLILFDEPL
                     VNLDYKLREELRQEMRELFEARHTIAIYATTEPNEALALGGTTTILHEGRVVQSGKTA
                     EVYHRPQQVLAAELFSEPAINLMPGRISGTEVSFADTVHFPLNPDLQDIAEGEYRFGV
                     RPSHIGLVPSNDDDLELAVTVELAEISGSETFLHVRNEQFVLVLHLPGVHEYDVDTPI
                     LIYIPTHKLFVFAADGQLIQAPSRRLGRTA"
     gene            11605..12717
                     /locus_tag="VFF22_15855"
     CDS             11605..12717
                     /locus_tag="VFF22_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007970463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX18326.1"
                     /translation="MAEIRLHNLAHSYSRDPKDPADYAIREMNHVWQQGGAYALLGPS
                     GCGKSTLLNIISGLLSPSHGEVQFDGKVVNQLHPEQRNIAQVFQFPVVYDTMTVFDNL
                     AFPLRNQGMNEAKVLAKVNEIAEVLELHPLLHKRARNLTADEKQKVSMGRGLVRDDVS
                     AILFDEPLTVIDPHLKWKLRRKLKQIHEQFNITMVYVTHDQLEASTFADKIAVMYGGQ
                     IVQFGTPRELFERPSHTFVGYFIGSPGMNLIDVQPCADGVRFNNTVLPLSPALNRRIA
                     ELGGQSLKIGIRPEFVHIWDGPNDDALCGEVVHLEDLGTYKILSLKLDGQLLKVRLQE
                     DQPVPQQRVYLSFPAQWLMLYADDFLVETQALEVQP"
     gene            12714..13577
                     /locus_tag="VFF22_15860"
     CDS             12714..13577
                     /locus_tag="VFF22_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZX18327.1"
                     /translation="MKVQNNKAWWLVLPVFLLVAFSAIVPMMTVVNYSVQDIFDHSTR
                     YFVGVDWYKQVLQDTRLHDSLLRQFIFSGCVLLIEIPLGIAIALCMPTKGRMASLVLI
                     VLAIPLLIPWNVVGTIWQIFGRADIGLLGWSLNSLGISYNYASNTSDAWVTVLIMDVW
                     HWTSLVALLCYSGLRAIPDVYYQAARIDRASSWAVFRHIQLPKLKSVLLIAVMLRFMD
                     SFMIYTEPFVLTGGGPGNATTFLSQTLTQMAIGQFDLGPAAAFSLVYFLIILLVSWLF
                     YTAMTHNDKNR"
     gene            13586..14386
                     /locus_tag="VFF22_15865"
     CDS             13586..14386
                     /locus_tag="VFF22_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018925489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZX18328.1"
                     /translation="MNLRKQVVLGLYILFLLVPIYWLLNMSFKSNSEILGGLSLWPHN
                     FTLANYKVIFTDPSWYEGYLNSLYYVSLNTVISLSVALPAAYAFSRYRFLGDKHLFFW
                     LLTNRMAPPAVFLLPFFQLYSSIGLFDTHIAVALAHCLFNVPLAVWILEGFMSGVPKE
                     IDETAYIDGYSFPKFFVKIFIPLIGSGIGVTAFFCFMFSWVELLLARTLTSVDAKPIA
                     AVMTRTVSASGIDWGVLAAAGVLTILPGMLVIWFVRNHVAKGFDLGRV"
     gene            14398..14670
                     /locus_tag="VFF22_15870"
     CDS             14398..14670
                     /locus_tag="VFF22_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011267211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2160 domain-containing protein"
                     /protein_id="HZX18329.1"
                     /translation="MDWMAWTLPTALFFGFIGMLLVGMTIWELRSPCIERKGLLPIAT
                     TRGDRLFIGLLGSAYLHLLVVGATDWSVWIASVLSLFWLFAVMRWG"
BASE COUNT         2783 a         4248 c         4665 g         3167 t
ORIGIN      
        1 cggctggggc acgataccct gagcggcacg ctctgctaca tgtttgcgat aggcttcaag
       61 cacagttatt accctcatca gtggtcccaa agggttgtcc gggacgcgat ccagaaaaca
      121 acaaaccagg cgcttcgtgg ctttatgggc cgctcagccg aggttttgct gctttcttgc
      181 agaagctgtt ttcaaagttt tacgccggca ggacgggctg gatgagggct ggtgccgaca
      241 cttgaagggg gctcaagggt cagcaccaac atcgaacctg caggattgac tgtgctcgtg
      301 acgctttgaa aacagcttcc aacggacgtt gctgcctcta aaaggccggc cgattctact
      361 ggaaagcttt ggataagtta agccaaaggc cccgtttatc tgggggtgat ctgccttagg
      421 caaagggcta agatgccagc ctgtccgcca gtacccatgc ctttcgttat gtccaaccaa
      481 cgtatcaaaa ccccttgtgt cggcctgtgc tccactgttt acggcgattt ggtctgccgt
      541 ggctgcaagc gcttccacca tgaggtgatc aactggaacg gctataacga cgatgaaaaa
      601 cgcgccgtgt ggctgcgtct ggaggtgctg ttggtgcagg tgatggcagc caaactggaa
      661 attttcgatg ccgatcgcct gcgcatgcag ctggaacagc gcaagatccg ctttgtgccg
      721 cagcagtcgc cgtactgctg ggcctatcaa ctgattgccc gtggcgcgcg ggtgatcagc
      781 cagttgaatg cctatggcat ggtgctgctg ccggagtttc gcgagtggaa cttgccggaa
      841 ttgcgtgatg cgattgatcg tgagttcttt ctgctgtccg aagcgcacta cgagcgctat
      901 atcgcgccgc gctttctgct cgaaggcctt gagataagag tctgaagcca tctttcaaat
      961 agaaaagccg cctgtttagg cggctttttc gttgcgcggc aggcggttag cgctgggcga
     1021 ccagttcttc caggtgggcg atgatgtcgt ccggcttgag caccaggatg tcgctttcca
     1081 gggaatcgag aatcacctcg gcggtattgc cgatcagcgc gccggaaata ccggtgcgcg
     1141 ccacggtgcc gatcaccgtc acgccagcgt tgagtcgatg ggctgtctgc gggatcaaca
     1201 catccgctgg gccttcggcg atatgcaggc gctcattgct gatctcgtac tcggcctgga
     1261 acgccttgca ttgctcgcgg tagcgcgcct cgatggtttc cttgagctgg aacgtcgggt
     1321 cggctgccga gagcatcggt gccgggtgag cgctgatcac gtgcagctcg gccttggcca
     1381 gggcggcaat atcaaagcca tggctgacga tgctggtgtg cagggtgcgg tgctcgctgt
     1441 cggcgttacc cacatcgacg gccgccagaa tcgcggtgcc cgcccaggat ttttcggttt
     1501 tcaccatcag cactggcgct gggcagtaac gcagcagctt ccagtcatcg ggggtgagca
     1561 gggccttttt cagcgggttg tcgggaaagt gctgcttgat caccaggcca cagccttcgg
     1621 cctgctgcac gttgatgatg gtctggtgct ggttttcatc ccaggcttgt tggctggagg
     1681 tgctgaaacc ttcgctgttg agcaagccag taaggtcgcg caggtagccg gtgtgatcac
     1741 ttttcttgtc gcacaccaga aggtgcaggt gcgattgagt gacgccggca atcagcttgg
     1801 cgcgtttcag ggccaggtct tccgtgtgat tgggctccat caccaccaga atgctgcgaa
     1861 tagcttgcat gatcatctcc atgtgcagtc agttgtactg tttatcacta tagatcgctg
     1921 ccactggctt gctgcttgat cggcgtcaat cgagtggctg ttcgcgcgct cattcgtata
     1981 atggccgcgc tctagcctta tcactcccgc ctgcctggtt ttgtgaattt atgtatctgt
     2041 tagccgcaac cgatgatctg ctgcgttgtt ttgccccttg tctgttggca tatcgccgtc
     2101 tggttacgga gacgcgccga tgaacctgcc ggaaattcac gagtttctcg gctgccgcac
     2161 gccggatgcc tggatcactg ccgctctggc tgaccagcag acactgctga ttgatcataa
     2221 gaactgcgaa ttcaaggccg ccagcacggc cttgagcctg atggccaagt acagcaccta
     2281 cgtcgacctg ctcaacgcca tgtcgcgtct ggcccgtgag gagctggtgc accatgagca
     2341 ggtactgcgc atcatgaaac gccgcaagat cggcctgcgc ccggtatcgg cggcgcgcta
     2401 cgcctcaggc ttgcgtaagg tggtgcgtac ccatgagccg cacaaactgg tggataccct
     2461 ggtggtcggc gcctttatcg aagcgcgcag ctgcgagcgc ttcgaggcct tggtgccgca
     2521 tctggatgaa gaactgggca agttctactt cggcttgctg aaaagcgagg cgcggcattt
     2581 tcagaactac ctgaagcttg cctatcagta cggcgatgaa cgcgatgtaa accaggccat
     2641 cgagcgggtt agggtggcgg agcaggaact gattgagtcg ccagataccg agatgcgctt
     2701 tcatagcggt gtgccaccgc ttcctctggt gatctaaagg gtaaagggcg cctggttaaa
     2761 actctgtttg tcgacctgca gtgcccaacc ctgtttgtcc cagtcgccca gcacgatgcg
     2821 ttgcgcaggt tggccgttga cctgcagctg atgggtggcg gggcgatggg tgtggccgtg
     2881 gatcagcgtg cgtacaccgt gctcggccat gatgcgcggc acttcgtccg gggtgacgtc
     2941 gacaatctcg ctggctttca tccgcgtctg tgtgcggctt tcattacgca gcttgcgcgc
     3001 cagtttttgt cgggtgctca gcggcaggtt gcgcaggata aacagcgaaa gcgggttgcg
     3061 cagccagcgg cgcagcttca tatagccgat gtcctgggtg cacaggctgt cgccgtgcat
     3121 cagcagcacg cgctcgccat tcagctcgat cacgcaggga tcgggcagca aggtgcagcc
     3181 ggcttcacgg cagaaggcct tgccgaggag aaagtcgcga ttgccatgca tcaggtagat
     3241 acgtgtgccg cgctcactga gttggcgcag agcttgggca atcgaacgtt gaaagggggt
     3301 gatggcatcg tcgccgatcc acacttggaa gaagtctccc aggatgtaca gcgcctgggc
     3361 ctgaaccgcg cgggtttcga gaaaatgcag aaacgcccgg ctgatgtccg ggcgctcctg
     3421 ttcaagatgc agatcggaga tcagtagaat cactcaacga tctcggcacg ctcgatgatc
     3481 acgtcatcaa ccggtacatc ttggtggccg gatttgctgg tggtggcaac ggccttgatg
     3541 gcgttgacca cgtccatgcc ttcagccact tcaccaaaca ccgcatagcc ccagccttgt
     3601 acggtcggcg cgctgtggtt gaggaagctg ttgtcgccaa cgttgataaa gaactgcgcg
     3661 gaggccgaat gtggctccat ggtacgggcc atggccacgg tgccgatctt gttggcgacg
     3721 ccgttgttgg cttcgttctt gatcggggcg cgggtggttt tctgcttcat gcccggctcg
     3781 aaaccgccgc cctggatcat gaagttgctg atcacgcggt gaaaaacggt gttgtcgtaa
     3841 tgaccttctt gtacgtactg cttgaagttg gccacggttt ccggggcttt gtcttcgaac
     3901 aggttgaggg tgataacgcc gtggttggtg tgcagcttga tcatgtaggt aatcgctctg
     3961 tcgataaatc cgtgagccgc tgaatttcca gcaaagggca ttgcagcggt tcttgggcct
     4021 gtcagggggt tgacagcttc ggctatgata agcgctttgt tttagtcggc ctaccctacg
     4081 ccgcgcacct gcatgatcaa ggaccccatg agcaagccag aaactgtcgc cgccgctaac
     4141 tttcttcgtc ccatcgtcca ggccgatctg gatgccggca agcacgcgaa aatcatcacc
     4201 cgttttccgc cggagcccaa tggctacctg catattggcc acgccaagtc gatctgcttg
     4261 aacttcggcc tggccaagga gtttggtggc gagtgcaacc tgcgctttga cgacaccaac
     4321 ccggccaagg aagaccagga atacatcgac gcgatcaaga gcgacgtgca gtggctgggc
     4381 tttcagtggg cgggcgaaga gcgctacgcc tccaactatt ttgaccagct gcatgcctgg
     4441 gctattgagc tgatcaaggc gggcaaggcc tttgtctgcg acctgaacgc cgaagaaatg
     4501 cgcgcctacc gcggcaacct caccgaaccg ggcaagaaca gcccgttccg tgagcgcagc
     4561 gtcgaggaaa accttgatct gttcgcccgc atgaaagccg gcgaattcgc cgatggcgct
     4621 cgttcgctgc gcgcgaagat cgacatgacc tcgccgaaca tcaacctacg cgacccgatt
     4681 ctgtaccgta ttcgccacgc tcatcaccac cagacgggcg acaagtggtg catttacccg
     4741 agctatgact ttacccacgg tcagtcggac gccatcgaag gcatcaccca ctcgatctgc
     4801 accctggagt ttgaagatca ccgcccgctg tacgagtggt tcctcgcgca actgccagtg
     4861 ccggcgcagc cgcgtcagta cgaattcgct cgccttaacc tgaactacac catcaccagc
     4921 aagcgcaagc tcaagcagtt ggttgatgag aagcacgtca atggctggga cgatccacgc
     4981 atgtcgaccc tctcgggttt ccgccgccgt ggctacaccc ctgcatcgat ccgtaccttc
     5041 tgcgacatga ttggcgtcaa ccgcgcaggc ggtgtagtgg atgttggcat gctcgaattc
     5101 gccattcgtg atgatctgga tgccaatgcc gcacgtgcca tgtgcgtgct caagccgctg
     5161 aaagtggtga tcaccaatta tccggaaggc aaggtcgaga acctggaact tgcgcgtcat
     5221 cccaagcagg acatgggcgt gcgtgtattg ccgttcagcc gtgagatcta tatcgacgcc
     5281 agcgacttcg aagaagtccc gccggccggc ttcaagcgct tgatccccgg tggcgaagcg
     5341 cgcctgcgcg gtagctacgt gatccgcgcg gatgaggcga tcaaggatgc cgagggcaat
     5401 atcgtcgaac tgcgctgcag ctacgacgaa aacaccctgg gcaagaaccc cgaaggccgc
     5461 aaggtcaagg gcgtgatcca ctgggtgccg gcggctgaaa gcgtcgagtg cgaagtgcgc
     5521 ctgtacgacc gcctgttccg ctccgccaac ccggagaagg acgaagaggg cggcagcttc
     5581 ctcgacaata tcaacccgga ttcgttggtg gtgctcaagg gctgtcgcgc cgagccatcg
     5641 ctggctgaag cgctgcctga agaacgcttc cagtttgagc gcgaaggtta cttctgcgcc
     5701 gatatcaagg acagcaggcc gggtgcgccg gtcttcaacc ggactgtcac cctgcgtgac
     5761 tcctgggcct aaggcccggt cgctctgatc aaaaccgggc cggtacgctg gcccgtggtt
     5821 tccaaggaac tgcaatgctc tcgatctaca acacgctgac caaaagcaaa gacgtcttca
     5881 aaccgctggt gggtaaccag gtgcgcatgt atgtgtgcgg catgacggtt tacgacttct
     5941 gtcatatcgg tcatgcgcgg gtgatggtgg cgttcgatgt ggtgactcgc tggctgcgcc
     6001 atcgcggcta tgaggtgacc tacgtacgca atatcaccga catcgacgac aagatcatca
     6061 agcgcgccca ggagaatggc gagccgttcg aggtgttggt cgagcgcatg attgcggcca
     6121 tgcacgaaga cgaggcgcgt ctgaatgtgc tgcgtccgga tcaagagcca cgtgccaccg
     6181 ggcatatcgc cggcatgcac gagatgatcc agaccctgat cgacaaaggc tttgcctatg
     6241 ccgcggacaa tggcgacgtg tactaccgcg tgggcaagtt tgttggctac ggcaagctgt
     6301 cgcgcaggaa gatcgaagac ctgaaaatcg gtgcgcgcat cgaggtcgat gaagccaagc
     6361 aggacccgct ggatttcgtt ttgtggaagg ctgccaagcc gggcgagccg agctgggagt
     6421 cgccgtgggg cgcggggcgt ccgggctggc atatcgaatg ctcggtgatg tccacctgct
     6481 gcctgggcga gaccttcgat attcacggcg gtggcccgga cttggtgttc ccgcaccacg
     6541 agaacgaaat cgcccaaagt gaagccgcca ctggcaagct ctacgccaat gcctggatgc
     6601 acgccggcgc ggtgcgcgtg gacggcgaga agatgtccaa gagcctgggc aacttcttca
     6661 ccattcgcga agtgctggag aaatatcacc cggaagtggt gcgctacctg ctggtgtcca
     6721 gccactaccg cagcccaatc aactattccg aagacagcct gcgcgaagcc aaaggcgcgc
     6781 ttgagcgttt ctacaatgcg ctcaagggcc tgcccgttgc tgtgcctgct ggcggtgaag
     6841 cctttgtcga gcgctttgct gcggcaatgg atgatgactt caactcgccg gaagcctgtg
     6901 ctgtgttgtt cgaactggcc cgcgagatca accgcctgcg cgacagtgat ctgcaggccg
     6961 ctgccggttt ggcagcgcgc ttgaaggaac tggctgcggt gctcggtgtg ctgcagcttg
     7021 aacctgaagc cttcctgcag gccggtgcgg ccggcaaggt cgatgctgcc gaggtggagg
     7081 cattgatcgc tgcgcgtctg caagcccgcg ccgagaagaa ctggggcgag tccgaccgta
     7141 ttcgcgatca gctcaccgcc atgggcgtgg tgctggaaga cagtaagggt gctaccacct
     7201 ggcgtctggc tgactagctc agctgctgga ttgcaaaggc cgctttcgag cggccttttt
     7261 tattgcgtgt ggtctgctag ttccacaacc agttccacaa ccccggcagg cgggcgtact
     7321 gcagtgggtc gccgagggcg cgtcttaagg cagcacgttg cagggcgtcg cgttggccat
     7381 agaacggctg cttgtctgtt gccaggccaa cgtcttccgc cacatccaga aggatctgca
     7441 ggtattcctg catatgccgc gcggtgtagg ggttgaggtc gtggaaggtc accactattg
     7501 ggacaacccc atcgacagcc ggtaaggcgc cgacgctgat ctgctgcgca gcgcgggcga
     7561 gctgatcacg cagattacta cgccgacgca ggctgccatt gatgccccag gtcttgccat
     7621 cgttggcgct caggtcggtc agcagcaaac gtaggccatg tttctggtag gcggcaaacg
     7681 tgcgctcgtc gtagttccag aagggtggcc gtaccaggcg cggtgccgcg ccgctgatgg
     7741 cggtcaggtc ggccacgccg tctttcaatg attgttccaa tgccgcaggg ctaaggaatc
     7801 ggtggttgga gtgccctgaa gtagcagtgt ggaaacctag caggtggcct tcacgttgtt
     7861 ggcggcgcag cagcgcttgc ccttgcacgc tgccaccggc cccggcggcg cgggtctgga
     7921 caaagaacag tgccttgatc ccaggctgga tcgggttgtc tgccagggtg tcgaggatct
     7981 gctcggttgg attgtcgcga gtgctggcgc tggggccgtc gtcgaaactc aggagaaagc
     8041 gtatcggctc aacctcgggg tttgggtgtg gcggtgcgag gctgctgcaa gcgcttaaca
     8101 gcgcgatggc cagcacggcg cagctccatc gcagtggctg cgagatttgc gtaaaaacgg
     8161 ttgttggagt tgcggggtaa cgcattaggc tttggctcgc gaagggacaa ggggttgcag
     8221 ggtggaacgc gcaggaggta gagggcgggg cgcggagtct atgcggctat ggccgcagtg
     8281 ggaaccgatg attagctatt aagggtttcg accctattgg ggccgtatag gtccatcgct
     8341 agtgaaaacg ctaacgatca caggcgctgc ggcgtgtttt agcgcacggt caggcccgtt
     8401 tcccggagcc gcttgtacag cgtgttgcgg ctaaccccga gactgcgcgc caggtgcgag
     8461 atattgccgc cgcaggcctg gtactggctg gccagatcat ccgcgctggt gctggccaaa
     8521 ggttgcggtg gctgctgggt ggcggctgct tgcgtagcag cgggctgcaa gtcggcaaag
     8581 aagtcatccg gcaggtgttc cggacggatc tgctggtcat cggccatggc cagggcaatc
     8641 tgcaggacgc tgctcagttg gcgcaggttg ccgggccacg ggtgacgctc gaacaggctc
     8701 agcacctcat ggcttagccc ggcccattgt tgcggttcgc ggtggtaatc ccagatgcgc
     8761 tggaccatgg cggccttgtc ggtgcgttcg cgcagcggcg gcagctccag attgaggccg
     8821 ctgatgcggt agtagaggtc ctggcgaaac agcccgcttt ccacatcctg gcgcagtggg
     8881 cggttggtgg cggaaatcag acggatatcc accgggtaca gctcactgct acccagcggt
     8941 tgtacgcagc gctcctgcag cactcgcagt agccgggcct gcacgcgtag tggcatatcg
     9001 ccgacttcgt cgagaaacag cgtgcctttg tcggccttgc ggatcaggcc gatgctgcct
     9061 ttctgattgg cgccggtaaa cgcgcctttt tcatagccga acagctcgga ctccaccagt
     9121 tcggcaggaa tggccgcgca gttgacggcg ataaacgcct ggctggcgcg tgagctggcg
     9181 tgatgcaggg ctttgacgaa gacttccttg ccgaccccgg tttcgccatg caccaaaatc
     9241 ggaatgttct tttccagaag gcgttcggcc tgacgcacgg ctttgtcgac gcgtgggtcg
     9301 ccgaggttga gcgaggccag ggtaaacgga ttgtgtttag cgctggcagg cggctgctgg
     9361 gcttggcgaa agtcgcgggc ctggatcggc gaaggtagct gcggacgttt gagctgcgcg
     9421 tgaaagcgaa agcgcccgct tgcgcgcagg ttgaacggca gtgcttcagg ctggttaagc
     9481 agttgctgca gcggcacgtc gaacaggctt tcgatattca cctgggccag gcccacaccc
     9541 agcaagctat cggcccggcg attggcggaa accacctggc cttgctcatc gaaaatcagc
     9601 agcccagccc aggggctgct gaggttatcc aggctggtgt tgaagctcag ctggaagtaa
     9661 cgctgctgga acaggttgag gatcagacgg ttttccaccg actggctcat catcttgacc
     9721 atgcccaggg tgtgcgaggg cggcagatag ctgtcactgg acacatccag caccgcaatc
     9781 atctgccgct gctcgtcaaa aatcggcgag gccgagccgg tcataaagcg gttggccttg
     9841 aggaaatgct catcgtgctg aatatgtacc gcttggccgc agcttagagc ggtgccgatg
     9901 gcattggtgc cggtgtagcg ctccagccag gtggctccgg cgacaaaacc agcggcgcgg
     9961 ttgggttcga taaagcgttg gtcgccccag gactgcagca cctggccctg atggtcggcc
    10021 agcatgatca ggcaattgga gttggccagg atggtgccgt agtagggcag cacttccttg
    10081 tgggtggtgt gcaccaaggc gtgctggctt tccagcagag cgctgacttc gccgcgctcg
    10141 ggctggctaa agatcggggt gctctggtgg gtcaggccgt agtcacggca gcgtgtccag
    10201 gagtcctgga tgatggtctc gtggggcagg gcagtgacag cgtcagccat gacagggatc
    10261 tcgcaagggg tcttgttgtt atgggccacg gtctgcgccg ggtccatgca gtgtcgccga
    10321 aggctgttca atgtcaacgc tgtgactgtt cagttgttca ctgtgtactg ttcaaagttg
    10381 ttcagtggtg ttttgcagag ttgtatgaag ggatatgtaa ttgttttaaa tcaatggctt
    10441 gcagattact caaagggttg gcacggaact cgccttgtag ctgggcagat aaaaagaaaa
    10501 aggacggccc atgtcactta ccctggagca tgtcacgcgt atcgtcgatc accagctgta
    10561 tatcgacgat gcctgcctga gcttcgaacc cggctccttc aatgtgctgc tggggcgcac
    10621 gctgtcgggc aagaccagcc tgatgcgcct gatggcgggt ctggataagc ccaccagcgg
    10681 acgcatcttg atgaatggcg cggatatgac caatgtctcg gtgcgccagc gcaacgtttc
    10741 catggtttat cagcagttca tcaattaccc gaccctgacg gtgttcgaga acattgcctc
    10801 gccgctgcgt caggccgggg tgagcaaggc cgagattctg gaaaaggtcc acgccacggc
    10861 gaagatgctg cgtatcgagg ccttgctcca gcgtcatccg ctggaactgt ccggcggcca
    10921 gcagcagcgc acggccatgg cccgggccct ggtcaaggat gcctcgctga tcctgttcga
    10981 cgagcccctg gtcaacctcg actacaagct gcgcgaggaa ttgcgccagg agatgcgcga
    11041 gctgttcgag gcccgtcaca ccatcgccat ctacgccacc accgaaccca acgaggccct
    11101 ggccctgggc ggcaccacca ccatcctgca cgaggggcgg gtggtgcaga gcggcaagac
    11161 cgccgaggtc tatcaccgcc cgcagcaggt actggccgcc gagctgttct cggagccggc
    11221 gatcaacctg atgccggggc gcatcagcgg caccgaagtg agttttgccg ataccgtgca
    11281 tttccccctc aacccggatc tgcaagacat cgccgagggc gaataccgct tcggcgtgcg
    11341 ccccagtcat atcggcctgg tgccctccaa cgacgatgat ctggaactgg cggtgaccgt
    11401 cgagctggcc gaaatcagcg ggtcggaaac ctttctgcat gtgcgcaacg agcagttcgt
    11461 gctggtgctg catctgcccg gggtgcatga gtacgacgtg gatacgccga tcctgatcta
    11521 tatccccacc cacaagctgt ttgtcttcgc tgccgacggg cagctgattc aggcccccag
    11581 ccgccgttta gggaggaccg cctgatggcc gagatccgtc tgcacaacct ggctcacagc
    11641 tacagccgcg accccaagga cccggccgat tacgccatcc gcgagatgaa ccacgtctgg
    11701 cagcagggcg gtgcctatgc gctgctgggg ccgtccggct gcggcaagtc gaccctgctg
    11761 aatatcattt ccgggctgct tagcccctcc catggcgagg tgcagttcga cggcaaggtg
    11821 gttaaccagc tgcacccgga acagcgcaat atcgctcagg ttttccagtt tccggtggtc
    11881 tacgacacca tgacggtgtt cgacaacctg gcctttccgt tgcgcaacca aggcatgaac
    11941 gaagcgaagg ttctggccaa ggtcaacgaa atcgccgagg tgctggaact gcacccgctg
    12001 ctgcacaagc gggcgcgcaa cctgaccgcc gacgagaagc agaaggtttc catgggtcgc
    12061 ggcctggtgc gtgatgatgt atcggcgatc ctcttcgatg agccgctgac ggtgatcgac
    12121 ccgcacctca aatggaagct gcggcgcaag ctcaagcaga tccacgagca gttcaatatc
    12181 accatggtct acgtcaccca cgaccagctg gaggcctcga ccttcgccga caagattgcg
    12241 gtgatgtacg gcgggcagat cgtccagttc ggcaccccgc gcgagctgtt cgagcggccc
    12301 agccacacct tcgtcggcta ctttattggc agcccgggga tgaacctgat cgacgtgcag
    12361 ccctgcgcgg atggggtgcg cttcaataac accgtgttgc cactgtcgcc ggcgcttaat
    12421 cggcgaattg ccgaactggg cgggcaaagc ctgaaaattg gtatccggcc ggagttcgtg
    12481 catatctggg acggccccaa cgacgatgcc ctgtgcggcg aggtggtgca cctggaagac
    12541 ctgggtacct acaagattct cagcctgaaa ctcgatggcc agctgctcaa ggtgcgcctg
    12601 caggaggacc agccggtgcc gcagcagcgc gtctatctga gctttccggc gcagtggctg
    12661 atgctctacg ccgacgactt tctggttgag acccaagcgc tggaggtgca gccatgaagg
    12721 tgcagaacaa caaagcctgg tggctggtgc tgccggtgtt tctgctggtg gcgttcagcg
    12781 ccatcgtgcc gatgatgacg gtggtcaact actcggtgca ggacattttc gaccattcca
    12841 cgcgctattt cgtcggggtc gactggtaca agcaggtgct gcaggacacc cgtctgcacg
    12901 actcgctgct gcgccagttc atcttttccg gttgcgtgct gctgatcgag attcccttgg
    12961 gcatcgccat cgccctgtgc atgccgacca aggggcgtat ggcctcgctg gtgctgatcg
    13021 tgctggcgat tccgctactg attccgtgga acgtggtggg caccatctgg cagatcttcg
    13081 gccgagccga catcggtttg ctcggctgga gcctgaacag cctgggtatc agctacaact
    13141 acgcctccaa taccagcgac gcctgggtca cggtgctgat catggacgtc tggcactgga
    13201 cctcgctggt ggcgctgctc tgttactccg gactgcgggc gattcccgat gtgtattacc
    13261 aggcagcgcg tatcgaccgt gcgtcaagct gggcggtgtt ccgccatatt cagctgccca
    13321 agctgaaaag cgtgctgctg atcgcggtaa tgctgcgctt tatggacagc ttcatgatct
    13381 acaccgaacc ctttgtgctg accggcggcg ggccgggtaa cgccaccacc ttcctcagtc
    13441 agaccctgac ccagatggcc atcggccagt tcgacctagg cccggcggcg gcgttctccc
    13501 tggtgtattt cctgatcatt ctgttggtgt cctggctgtt ctacaccgcc atgacccaca
    13561 acgataagaa ccgctgaggc cgaccatgaa cctacgcaaa caagtggtgc tggggctcta
    13621 tattctgttc ctgctggtgc cgatctactg gctgctgaac atgtcgttca agagcaacag
    13681 cgaaatcctc ggtggcctga gtctctggcc ccataacttc accctggcca actacaaggt
    13741 gatcttcacc gaccccagct ggtatgaggg ctacctcaac tcgctgtact acgtcagcct
    13801 caatacggtg atctccctga gcgtggcgct gccggcggct tacgccttct cgcgctaccg
    13861 ctttctcggc gacaagcact tgttcttctg gttgctcacc aaccgcatgg ctccgccggc
    13921 ggtgtttctg ctgccgttct tccagctgta ttcgtctatc ggcctgttcg atacgcatat
    13981 tgccgtggcg ctggcgcact gcctgttcaa cgtgccgctg gcggtgtgga ttctcgaagg
    14041 ctttatgtcc ggggtgccga aggaaatcga cgaaacggcc tatatcgacg gctacagctt
    14101 cccgaaattc ttcgtgaaga tctttatccc gctgattggc tcgggtatcg gcgtcacggc
    14161 gttcttctgc tttatgtttt cctgggtcga gctgctcttg gcgcgcaccc tgacctcggt
    14221 ggacgccaag cccatcgccg cggtgatgac gcgcaccgtc tcggcctcgg gtatcgactg
    14281 gggtgtgctg gccgcggcgg gggtgttgac catcctgccg ggcatgctgg tgatctggtt
    14341 tgtccgcaac cacgtggcca agggctttga cctgggccgg gtataaggag aaaaacgatg
    14401 gattggatgg cctggactct gccgacggcg ttgtttttcg gctttatcgg catgctgctg
    14461 gtcggcatga cgatctggga gctgcgttcg ccctgcattg agcgcaaagg cttgttgccg
    14521 attgctacca cccgtggtga tcggctgttt atcggtctgc ttggaagtgc ctacctgcac
    14581 ctgctggtgg tgggcgcgac cgattggagt gtctggatag cctcagtgct gtccctgttc
    14641 tggttgtttg cagtgatgcg ttggggctga accgggctgc cctgggcgcg cctgctggtt
    14701 gggcgcggcc atgacccgct tgcctcattc caaaccctga gatggaggtc tctatgttcg
    14761 acaataacaa caaaactcga catagcatgg cgctggctgc cctgctgacg ttgtccggct
    14821 tgagcggcgc agcctgggct gatgcctacg aagaagcggc aaa
//
LOCUS       DASRUM010000238         4422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2289724, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000238 DASRUM010000000
VERSION     DASRUM010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2289724"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1564)
                     /locus_tag="VFF22_15875"
     CDS             complement(<1..1564)
                     /locus_tag="VFF22_15875"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZX18330.1"
                     /translation="MIEQEDPSRDRLKQHFAQRVINQARQVLEVWQRLQRSEWSEPGM
                     RELSEATQLLQRYAERFEQTEHSQLAQEIDNCLQLVANNRGRLNSELISQLNELLQRL
                     SRTGLRHGDRFEQTVLPPLRKPVYLALQHLERAEQLVQRLEFFGMNAIALDSANAFRN
                     AMRERHPAAILMEVDFSGPGCGLLLAKSVQEGLEHPIPLLFFSPEDTDTMTRLSAVRA
                     GGQEFFTGSLDASNLFERIEVLTHVSQYEPYKVLIIDDSRAQATHTERVLNSASIVTR
                     TLIEPIQAMSHLADFQPDLIILDMYMPECNGPELAQVIRHNDRYVSVPIIYLSAEDDL
                     DKQLDAMSEGGDDFLTKPIKPRHLIATVRNRAARARSLKARMVRDSLTGLYNHTHTLQ
                     LLEDARFRAERDGQPLSFAMIDIDFFKKVNDTYGHPMGDRVIKSLALFLKQRLRKSDH
                     IGRYGGEEFAVVMPDTDAESARRVLDDIRQRFAEIQYSAQPHDLSCTFSCGIAQLAPQ
                     LDGKLLSQQADLA"
     gene            1874..2947
                     /locus_tag="VFF22_15880"
     CDS             1874..2947
                     /locus_tag="VFF22_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015275648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2333 family protein"
                     /protein_id="HZX18331.1"
                     /translation="MLDWKNRTAGASDSAAGAANTSTSKSTGSLAARTVGGLLGLYLI
                     VALVVGWYWSQEPAQFSVQQRAEAASQARGHKLVNGYTTAETLKQVANTLLDKPGGYL
                     SNDLAPPGLWLDNMPSWEFGVLVQVRDMSRALRKDFTRSQSQSTEDPDLAKAEPRFHF
                     DNKSWALPASEAEYAEGIKSLDRYLARLADPSQSNAQFYTRADNLNNWLGDAATRLGS
                     LSQRLSASVGQVRLNTAVVDPALAENVAKADFIEGVNYETPWLQIDNVFYEARGQAWA
                     LAHLLRAIEVDFADVLAKKNATVSVRQIIRELEAAQEPLWSPMILNGSGYGLLANHSL
                     VMANYISRANAGLIDLRQLLSQG"
     gene            2951..3487
                     /locus_tag="VFF22_15885"
     CDS             2951..3487
                     /locus_tag="VFF22_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZX18332.1"
                     /translation="MAWSEAEVAHRAASDAERIAWVDEDDRPLGGVLRSELRARHLLG
                     RGTYILLFNSAGLLCVHRRTLSKALYPGYWDVAAGGMVLEGESYAESAARELAEELGI
                     EDAALVEHAHFLYDAPESRLWCMAYSAVSDAPLRLQPEEVLEARFISIDEALAETQHL
                     PYCPDSLAALQRYLAAIA"
     gene            3819..4190
                     /locus_tag="VFF22_15890"
     CDS             3819..4190
                     /locus_tag="VFF22_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor Sui1"
                     /protein_id="HZX18333.1"
                     /translation="MVKKASSFAALSGLVYSTDSGRHCPDCSQPVDACICKQTLIPEG
                     DGIARVRRETKGRGGKTVTTISGVPLAEEPLKDLVSALKKRCGCGGSLKDGVIEIQGD
                     HVELLMAELIKHGFKAKKSGG"
     gene            4315..>4422
                     /locus_tag="VFF22_15895"
     CDS             4315..>4422
                     /locus_tag="VFF22_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18334.1"
                     /translation="MSARRTRKDDGSQWTVADSRSVYGIRHWGAGYFAIN"
BASE COUNT          788 a         1290 c         1424 g          920 t
ORIGIN      
        1 gcgccaggtc ggcctgctgt gagagcagtt tgccatccag ttgcggcgcc agttgggcga
       61 tgccgcagct gaaggtacag gacaggtcat ggggctgcgc tgaatactgg atttccgcga
      121 agcgctggcg gatgtcgtcg agtactcggc gggccgattc ggcgtcggta tcgggcatca
      181 ccacggcgaa ctcttcgccg ccgtaacggc cgatatggtc gctcttgcgc aggcgctgct
      241 tgagaaacag cgccaggctc ttgatcaccc ggtcgcccat ggggtggccg taggtgtcgt
      301 tgaccttctt gaagaagtcg atatcgatca tcgcaaagct cagcggctgg ccgtcgcgtt
      361 cggcgcgaaa gcgtgcatct tcgagcaatt gcagggtgtg ggtgtggttg tacaggccag
      421 tcaggctgtc acgtaccatg cgcgccttga ggctgcgcgc acgggcagcg cggttgcgta
      481 cggtggcgat cagatggcgt ggcttgatcg gcttggtgag aaagtcgtcg ccgccttcgc
      541 tcatggcgtc gagttgcttg tccaggtcgt cttcggcgga caggtagatg atcggcacgc
      601 tgacataacg gtcgttgtgg cggatcacct gggccagctc cgggccgttg cactcgggca
      661 tgtacatgtc gaggatgatc aggtccggct gaaaatccgc cagatggctc atggcctgga
      721 tcggctcaat cagggtgcgg gtgacgatgc tggcgctgtt gagcacgcgc tctgtatgcg
      781 tagcctgggc ccgcgagtcg tcaatgatca gcaccttata gggctcgtac tgcgagacgt
      841 gggtcagcac ctcgatgcgc tcgaacaggt tcgaggcgtc taggctgcca gtgaagaact
      901 cctggccgcc ggcacgcacg gcagacaggc gtgtcatggt gtcggtgtct tccgggctga
      961 agaacagcag cggaattggg tgctctaaac cttcctgcac gcttttggcc agcaacagac
     1021 cgcaacccgg gccagagaag tcgacttcca tcagaatcgc cgccgggtgc cgttcgcgca
     1081 tggcattacg gaaggcgttg gcgctgtcca gggctatggc gttcatgccg aagaattcca
     1141 ggcgctggac cagctgttcc gcgcgctcta gatgctgcag agccaggtaa accggtttgc
     1201 gcagcggcgg cagcacggtt tgttcgaagc gatcgccgtg gcgcaggcca gtgcgtgaca
     1261 ggcgctgtaa caattcgttg agttggctga tcagttcgct attgagacga ccacggttgt
     1321 tggccaccag ttgcaggcag ttatctattt cctgggccaa ctggctgtgt tcggtctgct
     1381 cgaaacgttc ggcgtagcgc tgcaacagct gcgtcgcttc gctcagctcg cgcatccccg
     1441 gctcgctcca ctcgctgcgc tgcaggcgct gccagacttc cagcacctgg cgtgcctgat
     1501 tgatcacgcg ctgcgcgaag tgctgcttga ggcggtcgcg gctggggtct tcttgctcga
     1561 tcatggcccg gtttcggttg gcgtagcgcg tggctacagg gtgatggctt agtgctagca
     1621 atgctaacac cggcagattt ttttgcgagc gccgtcgatc tattggcgtc ggccttctgt
     1681 ctatgacatc acagatacgt aatcggccta accggcgcta agtttgccgt tcgccgctgc
     1741 gcagcgggcc acgcttccct tatagtgcaa ggcagttgcg caaccctgct tgagggttgt
     1801 ggtcgaacca acagccaccg ggctgtgcag tccggtcgcg agcttgctac ttgggtaaca
     1861 aaaggacatt gccatgctgg attggaagaa ccgaactgcc ggagcgagtg atagcgcggc
     1921 aggtgccgcc aacacctcaa cgagcaaatc cactggaagc ctggccgcac gtacggtcgg
     1981 cggcctgttg ggactctatc tgattgtcgc cctggtggtg ggctggtact ggagtcagga
     2041 gccggcgcaa ttctccgtgc agcagcgcgc cgaggcggcg tcccaggccc gcgggcacaa
     2101 gctggtgaat ggttacacca ccgctgaaac cctcaagcag gtggccaata ccttgctcga
     2161 caagccgggc ggctacctgt ccaacgacct cgcaccgccg ggcctgtggc tcgacaacat
     2221 gccgagttgg gagtttggcg tgctggtgca ggtgcgtgat atgtcccgag cgctgcgtaa
     2281 ggacttcacc cgttcacagt cgcaatccac cgaagacccg gatctggcca aggccgagcc
     2341 gcgctttcac ttcgacaaca agagctgggc gctgccggcc tccgaggctg aatacgccga
     2401 aggcatcaag tcgctggacc gctacctggc ccgtcttgcc gatccgagtc agtccaatgc
     2461 acagttctat acccgcgccg acaacctgaa caactggctg ggcgatgccg ccacgcgtct
     2521 gggttcgctg tcgcagcgcc tgtcggcgag tgtcggtcag gtgcgtctga atactgcggt
     2581 ggttgatccg gcactggccg aaaacgttgc aaaggctgac tttatcgagg gcgtgaacta
     2641 cgaaacgccg tggttgcaga tcgacaatgt gttctacgaa gcccgtggtc aggcctgggc
     2701 tctggcccat ttgctgcgtg ccatcgaggt ggattttgcc gatgtgctgg cgaagaagaa
     2761 cgccactgtc agcgtacggc agatcattcg tgagctggag gccgcgcagg aacccctgtg
     2821 gagcccgatg atcctcaacg gaagcggtta tggcctgctg gccaaccact cgctggtgat
     2881 ggccaactac atctcgcgcg ccaatgccgg gctgatcgac ctgcgccaac tgctgtcgca
     2941 gggctaagcg gtggcctggt ccgaagctga ggtggcgcac cgcgctgcct ccgatgccga
     3001 acgcatcgcc tgggttgacg aagatgatcg accgcttggc ggtgtgttgc gcagtgaatt
     3061 gcgcgcgcgg catctgcttg ggcgcggcac ctatatcctg ttgttcaaca gcgccggcct
     3121 gctctgcgtg catcggcgca ccttgagcaa ggcgctgtat ccgggttatt gggatgtggc
     3181 ggctggcggc atggtgctcg aaggcgagag ctacgccgaa tccgccgcgc gcgagctggc
     3241 tgaagagctg ggtatcgagg atgcggcgct ggtcgagcat gcacattttc tctacgacgc
     3301 cccggaaagc cgtctgtggt gcatggccta ttcggcggtg tctgatgcgc cgctgcgctt
     3361 gcagccggag gaagtgctgg aagcgcgctt tatctccatc gacgaggcat tggccgagac
     3421 gcagcacctg ccttattgcc ccgactcgtt ggccgccttg cagcgctatc tggccgccat
     3481 tgcgtaggat gggttgagcg tagtgatacc catgcgattt tggcctctcc ctcatcctta
     3541 aattgatggg ttacgcctgc ggctaaccca tcctacgctg agcgttgtcc gagattttga
     3601 gcgctgctgt aaggcatcct gaacaacgtc gccaatccgc cgattaatgg cgcattttta
     3661 cacttagcag ctgcggtttt tgccgttaca ctgcgccgcc ttcaagccca ggccgtgtat
     3721 cgccagggtg cgctgcccct gccagagtgg ggcttcgcgg tcgatacgcc cagcacgtgc
     3781 cgaccagtcg ctatcctgac cccttcgagg actaaccggt ggtcaagaaa gcttcctcat
     3841 ttgccgccct cagcggcttg gtgtattcca ccgacagcgg ccggcattgc ccggactgca
     3901 gccagccggt ggacgcctgc atttgcaaac agaccctgat ccccgaaggt gacggcatcg
     3961 cccgcgtgcg ccgcgagacc aaaggtcgcg gcggcaagac ggtgaccacc attagtggcg
     4021 tgccgttggc cgaagagccg ctgaaggacc tcgtcagcgc cttgaagaaa cgctgcgggt
     4081 gcggcggttc gctcaaggat ggggtgatcg agattcaggg tgaccacgtc gagctgctga
     4141 tggccgagct gatcaagcac ggttttaaag ccaagaagtc cggcggctga ccccgtcttt
     4201 tctaaacttc ctcggcggac cttgggtcac agctgagtaa tcgtcatttc cagactttaa
     4261 tctgtacgcg tcttggctgt gccaaggcca ctatcgccag caagggagac atcgatgtct
     4321 gcacgacgca cacgtaaaga tgacggcagc cagtggaccg tagcggacag ccgcagcgtc
     4381 tatggtattc gccattgggg cgcgggttac ttcgcgatca at
//
LOCUS       DASRUM010000239        18554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1953583, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000239 DASRUM010000000
VERSION     DASRUM010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 18554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18554
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953583"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1121)
                     /locus_tag="VFF22_15900"
     CDS             complement(81..1121)
                     /locus_tag="VFF22_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HZX18335.1"
                     /translation="MSKPGYRFALFATALAVVVVLLGAYTRLTHAGLGCPDWPGCYGF
                     IGVPMSEHKQTLAEARFPEAPVEVAKGWYEMIHRYFAGALGLVILGLAVQALRRRGEV
                     GQPLKLPLILLGLVTLQAAFGMWTVTLKLWPQVVTAHLLGGFATLSLLFLLTLRLSGK
                     STALVNLPARLRSLAALGLLLVVGQIALGGWVSSNYAAVACVDLPTCHGEWWPAMDFA
                     NGFHLTQHIGPNYLGGQLDSDARTAIHMTHRIGALLVTLVLLLLAWQLKRYGLARLAG
                     LLVLALTVQISLGISNVVFHLPLLVAVAHNLGGAALLLTLVLINYRLRSPARLASPAT
                     DTPVGFTALPGK"
     gene            complement(1147..1731)
                     /locus_tag="VFF22_15905"
     CDS             complement(1147..1731)
                     /locus_tag="VFF22_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18336.1"
                     /translation="MNPAIALPEVSPSQRRRGRLQLILILAVVIGPMILASAMYQWRF
                     WVPETRSYHGELIGTGQTRADLGVVGADEQRWQLLVTVPDVCDAACKKLVFLARQVHI
                     GLNRDVARAAHGLATAQPIAGDYDAQLRREYPQLTRYQLQLDAYGKTAGKAEGAQLWI
                     VDPRGNLVLRYDATSKGKAILNDLRHLLKISQIG"
     gene            complement(1706..2437)
                     /locus_tag="VFF22_15910"
     CDS             complement(1706..2437)
                     /locus_tag="VFF22_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HZX18337.1"
                     /translation="MSAFRPGWLPSVLVLLLFPCLIALGFWQLARAEEKRELLAAHQA
                     QQQSAPISIDELEQHPDPAYKRVQLQGFFDARHTLLLDNRTRNGKVGVEVLQPFYDQT
                     SGLWLLLNRGWLPWPDRRISPTFATPDTPLRLQATVYVPLGEAFQLQGAQPANGWPRL
                     VNEVKPDALWQQLGRGGLSYEVRLDPGPASFQGDWPVIAMSPDKHLGYAVQWFALAAA
                     LLCLFIYLGLSNARETRHEPSHRPA"
     gene            2528..2731
                     /locus_tag="VFF22_15915"
     CDS             2528..2731
                     /locus_tag="VFF22_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HZX18338.1"
                     /translation="MLKAAIILLLLATLVSLFSGLFFLVKDESQTSRVVNSLTVRVIL
                     TALTVALIAWGFYSGQLVSQVTW"
     gene            complement(2818..3714)
                     /locus_tag="VFF22_15920"
     CDS             complement(2818..3714)
                     /locus_tag="VFF22_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003303656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HZX18339.1"
                     /translation="MSSHESHESYYVPAQSKWPIIATLGMLISVYGVGTWFNDIKADR
                     ADSNGPLIFFVGALFLVYMFFGWFGNVIKESHAGLYSPQMDRSFRWGMSWFIFSEVMF
                     FAAFFGVLFYVRYWAGPWLGGEGDKGIANMLWPGFEYTWPMLNTPDPKLYPAPSGTIS
                     PWGLPLLNTILLVSSSFTLTFAHHALRKNHRKPLKIWLALTVLLGLAFLVFQVEEYVH
                     AYTELGLTLGSGIYGATFFMLTGFHGAHVTFGAIMLTVMLVRVMRGHFTADQHFGFEA
                     AAWYWHFVDVVWIGLFVFVYVL"
     gene            complement(3727..4278)
                     /locus_tag="VFF22_15925"
     CDS             complement(3727..4278)
                     /locus_tag="VFF22_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HZX18340.1"
                     /translation="MSEALSTRRLVIRLSLVVVGMFGFGFALVPIYDVMCQAFGINGK
                     TAGAYEAGLQNTDESRQVRVQFLATNAAEMVWEFGPEADEVLVHPGDSTQMLFVAYNP
                     TDKPMTAQAIPSVAPSKAAAYFHKTECFCFTQQVLQPGERIEMPVRFIVDRDLPADVR
                     HLTLAYTLFDITERKPPVAQATP"
     gene            complement(4290..5894)
                     /gene="ctaD"
                     /locus_tag="VFF22_15930"
     CDS             complement(4290..5894)
                     /gene="ctaD"
                     /locus_tag="VFF22_15930"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HZX18341.1"
                     /translation="MSAVIDPGHAGHGHAGHDHHHGPAKGLMRWVLTTNHKDIGTMYL
                     WFSFAMFLLGGSMAMVIRAELFQPGLQIVQPEFFNQMTTMHGLIMVFGAVMPAFVGLA
                     NWMIPLMIGAPDMALPRMNNFSFWLLPAAFGMLISTLFMEGGGPNFGWTFYAPLSTTY
                     APESVTFFIFAVHLMGISSIMGAINVVATILNLRAPGMTLMKMPLFVWTWLITAFLLI
                     AVMPVLAGCVTMMLMDIHFGTSFFSAAGGGDPVLFQHVFWFFGHPEVYIMILPAFGAV
                     SAIIPAFARKPLFGYTSMVYATASIAFLSFIVWAHHMFTVGIPLTGELFFMYATMLIA
                     VPTGVKVFNWASTMWRGSMTFETPMLFSVAFVILFTIGGFSGLMLAIAPADFQYHDTY
                     FVVAHFHYVLVPGAIFGIFASAYYWLPKWTGHMYDETLGKLHFWMSFIGMNMAFFPMH
                     FVGLAGMPRRIPDYNMMFANFNMVSSIGAFMFGATQLLFLFIVIKCIRGGKPAAAKPW
                     DGAEGLEWTVPSPAPYHTFSTPPEVK"
     gene            complement(5899..7023)
                     /gene="coxB"
                     /locus_tag="VFF22_15935"
     CDS             complement(5899..7023)
                     /gene="coxB"
                     /locus_tag="VFF22_15935"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HZX18342.1"
                     /translation="MMRHPRVWMGLLLWLVFSSAHASWGVNMAPGATEVSRSVFDLHM
                     TIFWICVVIGVIVFGAMFWSMLVHRRSTGQQPAHFHESTTVEILWTVVPLVILVLMAI
                     PATKTLIEIYDSSESELDIQVTGYQWKWHYKYLGEDVEFFSNLTTPQDQINNKATKGE
                     HYLLEVDEPLVVPVGTKVRFLITAADVIHSWWVPALAVKKDAIPGFINESWTRIDEPG
                     IYRGQCTELCGKDHGFMPVVVEAKSKEDYAAWLAERKVAAAAERELTTKEWTMDELMA
                     RGEKSYQTNCASCHQPTGEGLPPMFPALKGSAIAKGEAKGHINIVVNGKPGTSMAAFG
                     KQLSEVDIAAIITYERNAWGNAVGDMVTPKDILAFKQAQE"
     gene            7385..8041
                     /locus_tag="VFF22_15940"
     CDS             7385..8041
                     /locus_tag="VFF22_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18343.1"
                     /translation="MNIAALHALIQRAHQHEASTGHLASQMQAHLGQLHPSIRLPNED
                     TQGVLQRFVSAYIEQVPELLQAAHQVSQEAGIDAQIKPVLKVAEQFFLQPPALMAGHE
                     GLEGLLDEAYLAHRLVEEVNDRYIAHLGAPLIPLDTTVANLIAHQLIGEAFANQLDEA
                     VHHAVDGMLNDASFAQDSVQAYRERLHNPQTDAAWQRWPCLSRQLGVELQLDETRAAG
                     "
     gene            complement(8071..8799)
                     /locus_tag="VFF22_15945"
     CDS             complement(8071..8799)
                     /locus_tag="VFF22_15945"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HZX18344.1"
                     /translation="MPYKHQLIPLQGRAGTESAEEALQSIVGGFKRFRSEIFPQQEEL
                     FKKLATAQNPRAMFITCADSRVVPELITQSSPGDLFVNRNVGNVVPAYGQMMGGVSTA
                     IEYAVLALGVQHIIVCGHSDCGAMKAVLAPETLDSMPTVKAWLRHSEVALKVVEANCG
                     CEGHDRLGILTEENVVAQLNHLCTHPSVAARVACGQLFIHGWVYDIETSDIKAYDAEK
                     GEFRQIGDGPLPMATPRARYLPSS"
     gene            complement(8902..9960)
                     /locus_tag="VFF22_15950"
     CDS             complement(8902..9960)
                     /locus_tag="VFF22_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HZX18345.1"
                     /translation="MSVSLPPRGRGTASNPHNRFAPQRIIASDDGWFQEVPPSRATEV
                     RCETAKSIITRNQSPDLPFDRSLNPYRGCEHGCIYCYARPSHAYWDLSPGLDFETKLI
                     AKTNAVALLEQQLSKPGYVCAPINLGSNTDPYQPIERQHLLTRNSLKVLLDYRHPVTI
                     VTKGSLILRDLDLLTELAQQRLVAVMISLTTLDDELKRIMEPRAAAPAARLRAIRVLR
                     DNRIPVGVLCSPMIPMINDMELEHLLAAAKEAGAQSANYMLLRLPREVAPLFEEWLQA
                     HYPQRAEHVLSLIRQSRGGALYDSRFGQRFRGEGAFAELLAQRFALASKRLGLNRRDG
                     FNLDCTRFCPPGGQLALL"
     gene            complement(10055..10963)
                     /locus_tag="VFF22_15955"
     CDS             complement(10055..10963)
                     /locus_tag="VFF22_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HZX18346.1"
                     /translation="MLELLLALWPLFALIVAGYYLRRWAFPNEAFWPGAERLNYFILF
                     PALLFSSLATAPLDNPALPRLALAVLLALGIAWLALLLVRRVRGWPAGRFGAFSQGIL
                     RFNTYLGLAAVGSLFGQEGLTLAAIMLALMVPTVNVLSVWSLTAERGVSARSLLLPIL
                     KNPLILACLGGTLVNLSGLGLPGGSDRLLSLLAAASLPLGLLCVGAALKPEQLGGEVP
                     ALLSNSLLRLLAMPVLAWVVAYGLQLPAMESTVLVLFFALPTAPTAYVLTRQLGGDSQ
                     LMAGIITLQTLLATASLPLLLLLVAG"
     gene            11075..11479
                     /locus_tag="VFF22_15960"
     CDS             11075..11479
                     /locus_tag="VFF22_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZX18347.1"
                     /translation="MSDIHQGGCQCGDLRYEFAAPLQDIAHCHCSICRRSTGGIVTTW
                     ITVPLASFRWLAGTPHEYASSASCTRYFCGQCGAHLALFTQLSPETLDVTVATLDEPE
                     QAPADRHIWVGSRLPWLQLDEQLPREDEENIP"
     gene            11587..12939
                     /locus_tag="VFF22_15965"
     CDS             11587..12939
                     /locus_tag="VFF22_15965"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HZX18348.1"
                     /translation="MRSGMRFRQILLAPLLSKPGAVLGLLLMLFVAFFNPFGLRDASD
                     RHSETWLLRMLAPFYSESGQEEVVVVLIDDQALMSMGASWPLRYGEQARLLRLLLSYQ
                     PKSLFVDLLYTQRRYDGGPTDSLQRALGSFREQNVPLILADYRDAQGRSLVLPEFAAM
                     ADTAPSNWSGYGERYPLLLPSPLGTLRTPALQLYQAACQAQDCSPEGFEAPLLVRWGY
                     WSDAGMRGFVDLAGCDVRGQRENWGQTFNMLVADAFRSLRSAGEVERPQPCPYTRTLY
                     ANQLRDPSVAEVLRGKHVLLGAHIRGIPDLVSSPVQGLLPGVYVHAMALDNLLTQGRS
                     YWRAAPDGWLGISLSDWLELALLLLAGLVALWIPAHPHTKGRFLCRCVSHYLFWFVLL
                     SSLVLLISVYLASCLHIAPFDWLGLLLMIGLFYAYLGEARVAEWWHQRTRRTSTEKES
                     "
     gene            12942..13307
                     /locus_tag="VFF22_15970"
     CDS             12942..13307
                     /locus_tag="VFF22_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18349.1"
                     /translation="MFRATVFGLLLCSVSAWAQEPLKIIGYPLEKVALYDEQGEFLGD
                     INRDQLPVPDVQVISFSETKNLLLIAVGERQVWLDPLDVKLNHGKTVAFDCRKVAETS
                     LLADSKTNAVMGYSKACGK"
     gene            13322..14983
                     /locus_tag="VFF22_15975"
     CDS             13322..14983
                     /locus_tag="VFF22_15975"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZX18350.1"
                     /translation="MRKEMTLAIACLLLFGCQQMPSTSKAPLQGKFIDSPRARPLALQ
                     PVTSNPWLNNALVDRLAGAADPDEVRGFGKVDLTEQMAQVESFQVPELQTYCDQVLSR
                     LLTHWPHAKPDIKVRFSASESFGARATADNVIYLNLGTLQAIESEDELAAMLGHEAAH
                     LLLGHLDREHFFNQQKKLLDASVTAVNLGVTLAGLESQKSGTSRKLVVGDPDAVGKTI
                     VQSSLSGVLLKAASDNFWNSSWTRAQEDEADLLSVDLLSRAGYSPRGAVHGIQRLQSF
                     EGSNVSQLQRYQAMQKQLFSQAVQKKGVGGLLEQGIQSGVTAAQLAAVDLWKDFGKTH
                     QLPAQREEALARYIASEYRSERRRSPQLASYQRQLLGGQSGKILAGQVLINRATKAID
                     KGDLNEARKLAKDVARGPAARSMRAQTLQFVLAVQQRRYKEALANLEQVPDSEWQYAS
                     LSSYDQIITLSLSLERQAQAERYLQRGQAHWGNQLMTPLEISVHLQGERVAQAQQAYQ
                     SCQSSTSKGLLAQCESAIGRFKPQQAPADPLQQLGLPSMGKLLGS"
     gene            complement(14985..16937)
                     /locus_tag="VFF22_15980"
     CDS             complement(14985..16937)
                     /locus_tag="VFF22_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HZX18351.1"
                     /translation="MRWPGAWLLGLALLTSTCSQAELRLQLAGAELSPAEHQASQQLL
                     QDALAALPPQLKQQLDRSVSVRWRSLPAQVYGRAGRFSGIELNAALLPGLSDGSSATT
                     LTSRPHGTVRRELLATVLHEITHLYDRAQLWPPVGRQLQRQCRQREQSLGAVGLPDHC
                     RGQTTRRFTLSDDPLLLDLAGWPQQVGRRGAREQDNHQVARSPDSYELSNSREFLAVN
                     MEYFLLDPSYACRRPSLSRYLGAHFGWVPANQQPCAEGLAYLNAGRDFARQPLGSIDP
                     QRVYQVDYLLAEANDALASRWGHSMLRLVICAPGRPRGPDCRLDLQEHLVLSYRAFVG
                     DVQLSSWDGLTGVYPSRLFVLPLEQVIEEYTKVELRSLASVPLKLDRTQLEQLVTRAA
                     EQHWSYDGDYWFISNNCAVETLKLLRSGSQHPRLHALDSIMPNGLLDTLVARDLADRS
                     VLNDPAEALRLGYRFDSFRDRYQAMFSVLQTQLPIEQTQVEDWLIQTADKRQPWFARA
                     DLRTSAALLLLEQAALRRQLLLAQNELKRRYLSGRDGVDPTLNKAGGALQQILANSGF
                     LSRPAELLEGGYGLPQRAEWQRLETESQARQQQLRQLSDSLDQEVRTLLEPARLAELQ
                     ATEANLAQLGTHLRTLHKAGGGLQLP"
     gene            complement(16934..17254)
                     /locus_tag="VFF22_15985"
     CDS             complement(16934..17254)
                     /locus_tag="VFF22_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020192357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2388 domain-containing protein"
                     /protein_id="HZX18352.1"
                     /translation="MRPILLSAVVAVVLFSAATQAQTLVATSNIIVRALDRTFDFTSD
                     TTTSVRDSKVVLAARDDAASFVASQGQIRGAQLEAAIGTIRSQVPEAQGASDLVLAEA
                     ILAL"
     gene            complement(17361..17678)
                     /locus_tag="VFF22_15990"
     CDS             complement(17361..17678)
                     /locus_tag="VFF22_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2388 domain-containing protein"
                     /protein_id="HZX18353.1"
                     /translation="MNAIKTLSLAALLALAGTASATSFVVTTDAVVGAVAATAEASSD
                     VSSSFTDDKVVLAARDDAAAFVASQGDIRGVRLEAALQHIRQQVPTLQGDDMQLATAI
                     LSL"
     gene            complement(17758..17964)
                     /locus_tag="VFF22_15995"
     CDS             complement(17758..17964)
                     /locus_tag="VFF22_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019826986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HZX18354.1"
                     /translation="MDRTLKHQPPVNTQVNSPLWLRVYAAFCGWQRNLRTRHQLARLD
                     SRQLADAGITEAQRQAELAKPFWR"
     gene            complement(18230..18445)
                     /locus_tag="VFF22_16000"
     CDS             complement(18230..18445)
                     /locus_tag="VFF22_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019826986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HZX18355.1"
                     /translation="MERTLSSDLFFDHSQSTGSSALPLRMLASVLQWQRRVVSRRQLA
                     RLDSRLLADAGISEAQRHAELSKPFWR"
BASE COUNT         3897 a         5550 c         5683 g         3424 t
ORIGIN      
        1 tccagataat cgcgccacgt ggcgtgggct tgctgttcgc gcactagagt agccataagg
       61 tctctcctcg attgttctgg ttatttgccg ggcagcgcag tgaagccgac gggcgtatct
      121 gtcgcggggg atgccagccg tgcagggctg cgcaaccggt aattgatcag taccagggtc
      181 agcagcagcg cagcgccgcc gaggttgtgc gccaccgcca ccagcagcgg cagatgaaac
      241 accacgttgc tgatgcccag gctgatctgc acagtcagcg ccagcaccag cagcccggcc
      301 aaccgcgcca ggccatagcg cttgagttgc caggccaaca gcagcagcac cagggtcacc
      361 agcagcgcgc cgatgcggtg ggtcatatga atggcggtgc gcgcgtcgct gtcgagttgg
      421 ccaccgagat agttggggcc gatatgttgg gtcaggtgaa agccgttggc gaaatccatc
      481 gccggccacc attcgccatg acaggtcggc aaatcaacgc aagcgaccgc cgcgtaattg
      541 ctgctgaccc agccaccgag ggcgatctgc ccaacaacca ataacagccc gagcgccgcc
      601 aggctgcgca gacgtgcagg tagattgacc aaggcggtcg atttgccgga tagacgcagc
      661 gttaaaagaa acagcaggct aagggtggca aagccgccaa gcagatgcgc cgtaaccact
      721 tgcggccaaa gtttcagggt caccgtccac atgccaaagg ccgcctgcag cgtcaccagc
      781 cccagcaaaa tcaacggcag tttgagtggc tgaccaacct cgccacggcg gcgcagcgcc
      841 tgtaccgcca ggccgagaat caccaaaccc agggcgccgg caaaatagcg gtggatcatc
      901 tcgtaccatc ccttggcgac ttcgaccggg gcttccggaa aacgcgcttc ggccagagtc
      961 tgtttatgct cgctcatcgg cacaccgata aagccgtagc agccaggcca gtccgggcaa
     1021 cccaggccgg cgtgggtcaa gcgagtatag gcaccgagca gcaccaccac gaccgccagc
     1081 gcagtggcga acagggcaaa acggtaaccg ggtttgctca tcatcagtcc ttagccgtgg
     1141 gcagcattaa ccaatttgcg agattttcag cagatgccgc aggtcgttga gaatcgcctt
     1201 gcccttgctg gtggcgtcat aacgcagcac cagattgccg cgcggatcga cgatccacag
     1261 ctgcgcgcct tcggccttgc cggcggtctt accgtaggca tccagctgca gctgatagcg
     1321 cgtcagctgc ggatattcgc gtcgcagctg ggcgtcatag tcgccagcga tgggttgggc
     1381 agtggccagg ccatgggctg cgcgggctac atcacggttt aggccgatat gtacttggcg
     1441 agcgagaaat accagcttct tacaggcggc atcgcagaca tccggcacgg tcaccagcag
     1501 ctgccagcgc tgctcatcgg cgccgaccac ccccaggtcg gcgcgggttt ggccggtgcc
     1561 aatcagttcg ccgtggtagc tgcgggtttc cggcacccag aagcgccact gatacatggc
     1621 gctggcgagg atcatcgggc caataacaac ggccagaatc aggatcagtt gcagccgccc
     1681 acggcggcgc tgactgggcg acacttcagg cagggcgatg gctgggttca tggcgagtct
     1741 cccgcgcgtt agatagacct aaatagataa acaggcagag caaggccgcc gccagggcga
     1801 accattgcac ggcataaccc aggtgtttgt cggggctcat ggcgataacc ggccagtcgc
     1861 cctgaaatga cgccggaccc ggatcgaggc gcacctcata gctcagacca ccacggccca
     1921 attgctgcca cagggcatcc ggtttgactt cgttgaccag ccgcggccag ccgttagccg
     1981 gttgcgcacc ttgcagctga aaggcttcac ctagcggcac atacaccgtg gcctgcaggc
     2041 gcagcggggt atccggggtg gcaaaggttg ggctgatgcg acggtccggc caaggcagcc
     2101 agccgcgatt gagcaacagc caaaggccgc tggtctggtc ataaaaaggt tgcagcacct
     2161 cgacgccgac cttgccgtta cgggtgcgat tgtccagcag cagggtgtgg cgcgcgtcga
     2221 agaaaccctg caactgcacg cgtttgtacg ctgggtcagg gtgttgctcc agctcatcta
     2281 tgctgattgg cgcggattgc tgctgggcct gatgtgcggc caacagttca cgcttttctt
     2341 cggctcgcgc cagctgccaa aagccgaggg cgatcaggca gggaaacaac agcagcacca
     2401 gtacgctggg cagccagcca gggcgaaaag cgctcataac gaccgcccta aagccctgca
     2461 caagctggct atactgcacc tgaacaacat ttcccttccc cccggaagtt acgcatggga
     2521 gatacgcatg ctcaaggccg cgatcattct gctactgctg gcgactctgg tgagcctatt
     2581 cagcggcttg ttctttctgg tcaaggatga gagccagacc tctcgggtgg tcaactccct
     2641 gaccgtccgg gtaatactca cagccctgac ggttgccctg attgcctggg gcttttacag
     2701 cggtcaattg gtctcgcagg ttacttggta ggttgatcgc cgaaccgcac cggcggttca
     2761 acaattcgcc ttggcgcccg ctgaggttcg ccgcgagcgg cgaaacctcg ctggtcgcta
     2821 gagcacataa acgaagacga acaacccgat ccacaccacg tcaacgaaat gccaatacca
     2881 ggcggccgct tcgaagccga agtgctgatc ggcggtaaag tgcccgcgca tcacccggac
     2941 caacatcacc gtcagcatga tcgcgccgaa ggtcacgtgg gcgccgtgaa agccggtgag
     3001 cataaagaaa gtcgcgccgt agataccgga gccaagcgtc aggcccagtt cggtgtaggc
     3061 atgcacatat tcctcgacct ggaacaccag aaacgccagc cccagcagca ctgtcaatgc
     3121 cagccagatt ttcagcggct tgcgatggtt cttacgcagc gcatggtgag caaaggtcag
     3181 ggtgaagctg gaggacacca gcaaaatggt attgagcagt ggcagccccc aagggctgat
     3241 ggtaccgctc ggtgccgggt agagcttcgg gtcaggggta ttgagcatcg gccatgtgta
     3301 ctcaaaaccg ggccagagca tgttggcgat gcctttgtcg ccctcgccgc ccaaccaggg
     3361 gccggcccaa tagcgcacgt aaaacagcac gccgaagaag gcggcgaaaa acatcacctc
     3421 ggagaagatg aaccagctca tgccccagcg gaacgaccgg tccatctgcg ggctatagag
     3481 gcccgcgtgg ctttccttga tcacattgcc gaaccagcca aaaaacatgt agaccaggaa
     3541 cagggcgcca acgaagaaga tcagcgggcc gttggagtcg gcccgatcgg ccttgatatc
     3601 gttgaaccag gtacctacgc catacaccga tatcagcatg ccgagggtgg cgatgatcgg
     3661 ccacttgctc tgggccggga cgtaatagct ttcgtgactt tcgtgactcg acatttattg
     3721 ttctccttat ggagtcgctt gtgccaccgg cggtttgcgc tcggtgatat caaacaaggt
     3781 gtaggccagg gtcaggtggc gaacatctgc cggtaagtct ctatcgacga taaagcgcac
     3841 cggcatttcg atgcgttcgc cgggctgcag tacctgttgg gtaaagcaaa agcattcagt
     3901 cttgtggaaa taagcagcgg ctttggacgg tgccacgctg ggaatcgcct gggcggtcat
     3961 cggcttgtcg gtggggttgt aggcaacaaa cagcatctgc gtgctgtcac ccggatgcac
     4021 caacacttca tcggcctcgg ggccgaactc ccagaccatc tcggcggcgt tggtggcgag
     4081 aaactgcacc cgtacctggc gcgattcatc agtgttttgc agtccagcct cataggcacc
     4141 ggcggttttg ccgttgatgc caaaggcctg gcacatcacg tcatagatcg gcaccagggc
     4201 gaagccgaag ccgaacatgc cgactaccac caatgacagg cggataacca ggcggcgggt
     4261 cgacagggcc tcgctcatcg gtcggctccc tatttcactt caggtggcgt gctgaaggtg
     4321 tggtaaggcg ctggcgacgg cactgtccac tccaaccctt cagcaccgtc ccaaggctta
     4381 gccgctgcgg gtttgccgcc acggatgcac ttgatgacga taaacaggaa cagcaactga
     4441 gtggcgccga acataaaggc gccaatgctc gacaccatgt tgaagttggc gaacatcatg
     4501 ttgtagtccg gaatccgccg cggcatgccg gccaggccaa caaagtgcat cgggaagaac
     4561 gccatgttca taccgataaa gctcatccag aaatgcagct tgccgagggt ttcgtcatac
     4621 atatgcccgg tccacttcgg cagccagtag taggccgagg cgaagatgcc gaagatcgcc
     4681 ccaggcacca gcacgtagtg gaagtgcgcc accacgaagt aggtgtcgtg gtactggaag
     4741 tccgccggag cgatggccag catcaggccg gagaaaccgc cgatggtgaa caggatgaca
     4801 aaggccacgg agaacagcat cggcgtttcg aaggtcatcg agccgcgcca catggtgctg
     4861 gcccagttga acaccttcac cccggtcggc acggcgatca gcatggtcgc gtacataaag
     4921 aacagctcac cggtcagcgg gatgccgacg gtgaacatat ggtgcgccca gacgataaac
     4981 gacaggaagg caatggacgc agtggcgtac accatcgagg tgtaaccgaa caatggctta
     5041 cgggcgaagg ccgggatgat cgcactgacc gcaccgaacg ccggcaggat catgatgtac
     5101 acctcggggt gaccgaagaa ccagaacacg tgttgaaaca gcactgggtc accgccaccg
     5161 gcggcactga agaaactggt gccgaagtgg atgtccatca gcatcatggt cacgcaaccg
     5221 gccagtaccg gcattaccgc gatcagcagg aaggcagtga tcagccaggt ccagacgaat
     5281 agcggcatct tcatcagggt catgccgggg gcgcgcaggt tgaggatggt ggcgaccacg
     5341 ttgatcgccc ccatgatcga actgatgccc atcaggtgca cggcgaagat aaagaaggtc
     5401 accgattccg gcgcgtaggt ggtcgacagc ggggcataga aagtccagcc gaagttcggt
     5461 ccgccgccct ccataaacaa tgtgctgatt agcatgccga acgccgctgg cagcagccag
     5521 aaactaaagt tgttcatccg cggcagggcc atatccggcg cgccgatcat cagcggaatc
     5581 atccagttgg ccaggccgac gaaagccggc atcaccgcac caaacaccat gatcaggccg
     5641 tgcatagtgg tcatctggtt gaagaactcc ggttgcacga tctgcaggcc cggttggaac
     5701 agttcagcac gaatcaccat ggccatgctg ccgcccagca ggaacatggc gaagctgaac
     5761 cacaggtaca tggtgccgat gtctttatgg ttggtggtca gcacccagcg catcaggccc
     5821 ttggccgggc cgtggtggtg gtcatgtccg gcatgaccgt ggccagcgtg gcccggatcg
     5881 atcactgcac tcatgtcctt actcctgagc ctgtttgaac gcgaggatgt ctttcggcgt
     5941 gaccatgtcg cctaccgcat tgccccaggc gttgcgttcg taggtgatga tcgcggcgat
     6001 atcgacttcc gagagttgct tgccaaaggc ggccatcgaa gtgcccggct tgccgttgac
     6061 cacgatgttg atatgccctt tggcctcacc cttggcgatg gccgagcctt tcaacgccgg
     6121 gaacatcggc ggcagccctt caccggttgg ctggtgacag gatgcgcagt tggtctggta
     6181 ggacttctcg ccacgcgcca tcagctcgtc catggtccac tctttagtgg tcagctcgcg
     6241 ttcggcggcc gctgccacct tgcgctcggc gagccaggcg gcgtagtcct ctttcgactt
     6301 ggcttcaacc accacgggca taaagccgtg atccttgccg cacagctcgg tgcactggcc
     6361 acggtagatg ccgggctcgt cgatgcgcgt ccaggattcg ttgataaagc cggggatggc
     6421 gtctttcttc accgccaggg ccggcaccca ccaggagtgg atcacatcgg cggcggtgat
     6481 cagaaaacgc accttggtgc cgactggcac caccagcggc tcatcgacct ccagcaggta
     6541 atgctcacct ttggtggcct tgttattgat ctggtcttgg ggggtggtca ggttgctgaa
     6601 gaactcgacg tcttcaccca ggtatttgta gtgccatttc cattgatagc ccgtgacctg
     6661 gatatccagc tccgactcgc tggagtcgta tatctcgatc agggtcttgg tcgccggtat
     6721 cgccatcagc accagaatca ccaacggcac gaccgtccag aggatctcga ccgtggtgct
     6781 ttcatggaag tgcgcgggct gctggccggt ggagcggcgg tggacgagca tcgaccagaa
     6841 catcgcgccg aacacgatca cgccgatcac gacgcagatc cagaagatgg tcatgtgcaa
     6901 atcaaatact gagcggctga cctcggtggc acccggcgcc atattgacgc cccagctggc
     6961 gtgcgccgag ctgaatacca accacaacag gaggcccatc cagactcgtg gatgtcgcat
     7021 cattgcgggt tccccttaat ttttcttgtt atcccgccga accgttcagt ggctaagcct
     7081 tcgcacattt cacggtgaaa atgcctgcga agactgtcca tgccgcagtc gattcggagt
     7141 tatcaggtac tgcctttcga attcgagtat agacacggac ttgcacctgg caatgcgaaa
     7201 gccgaaatga acaacagacg gcaaggcacg cgctagagac cgcggctggc gcgggatagg
     7261 ccaaggtgaa cgcggcggca tatagccgac tcgcataact gtttaataat tatgacaatc
     7321 gggtcttagc cgaggcgctc agccagctaa ggttctcgcc catgtcctca tttcaggagc
     7381 agtcatgaac atcgccgctt tgcacgcact gatccagcgt gcgcaccaac acgaagccag
     7441 tacgggtcac ctggccagtc agatgcaggc ccacttgggg caactgcatc cgtccatccg
     7501 cctccccaac gaagacactc aaggcgtgct gcagcgtttt gtcagcgcct atatcgagca
     7561 ggtaccagag ctgttgcaag ccgcccatca ggtgtcgcaa gaagcgggga tcgatgcgca
     7621 gatcaagccg gtgctcaagg ttgccgagca gttttttctg cagccgcctg cgctgatggc
     7681 cggtcatgaa gggctggagg gattactcga cgaggcttat ctggcccatc gcctggtcga
     7741 agaagtgaat gatcgctaca tcgcccatct gggcgcgccg ctgatcccgc tggataccac
     7801 ggtggccaat ctgatcgccc accaactgat cggcgaagcc tttgccaacc agttggatga
     7861 ggccgtacac catgcagtcg acggcatgct caacgatgcc agctttgctc aggattcggt
     7921 acaagcctat cgcgagcgcc tgcacaaccc acagaccgac gcggcctggc agcgctggcc
     7981 gtgcctatcg cggcagctcg gggttgagct gcaactggat gaaacccgcg cggcgggtta
     8041 gcaaaccaaa cccagcgcgc gtttgctgaa ttagctgctc ggcagataac gcgcgcgcgg
     8101 ggtagccatc ggcagcgggc catcaccgat ctgacggaat tcgcccttct ccgcgtcgta
     8161 ggccttgata tcgctggttt cgatgtcata cacccagccg tggataaaca gctggccgca
     8221 ggccacccgc gccgccaccg acgggtgggt acacaggtga ttgagctggg ccaccacgtt
     8281 ctcctcggtg agaataccca gcctgtcatg gccttcacag ccgcagttcg cctcgaccac
     8341 cttgagcgcc acttcgctgt ggcgcagcca ggccttcaca gtcggcatgc tgtccagagt
     8401 ttcgggggcc agcactgcct tcatcgcacc gcagtcggag tggccgcaga cgatgatgtg
     8461 ctgcacgccc aacgccagca ccgcgtactc gatagcggtg gacaccccac ccatcatctg
     8521 accgtaggcc ggcaccacgt tgccaacgtt gcgattgacg aacaggtcgc ccggcgagct
     8581 ttgggtgatc agctcaggca ccacccgcga gtcggcgcag gtgataaaca tcgcgcgggg
     8641 attctgcgcg gtggcaagtt tcttgaatag ctcttcctgc tgcggaaaaa tctcgctgcg
     8701 aaagcgtttg aaaccaccaa cgatgctctg cagggcttcc tcggcactct cagtacctgc
     8761 gcgcccctgc agcgggatca actgatgctt gtatggcatg tttgcggcct ctatgaacct
     8821 gaataaacgc tgctaaaacg acagccaagg ccgctagcga gtcacatgcc aagtgttacc
     8881 gtccatggcc aaaacgacaa cctaaagcaa tgccagctgg ccacccggtg gacagaagcg
     8941 cgtgcaatca aggttgaagc catcacgtcg attcaagccc aagcgtttgc tggccagggc
     9001 aaagcgttga gcgagcaatt cggcaaacgc cccttcaccg cgaaaacgct gaccgaagcg
     9061 gctgtcatac agcgcgccgc cgcggctctg ccggatcaag ctgagcacat gttcggcgcg
     9121 ctgcgggtaa tgcgcttgca accattcctc gaacagcggc gccacttcac gcggcaggcg
     9181 cagcagcatg tagttggcgc tctgcgcgcc ggcctccttg gccgccgcca acaagtgctc
     9241 cagctccatg tcgttgatca tcggaatcat cggcgagcac agcacaccca ccggaatgcg
     9301 gttgtcgcgc agtacgcgaa tcgcccgcag acgcgccgcc ggtgccgctg cacgcggttc
     9361 catgatgcgt ttcagctcat catccagggt ggtcaggctg atcatcaccg ccaccaggcg
     9421 ttgctgtgcc agctcagtga gcagatcgag gtcgcgcaga atcagcgaac ccttggtaac
     9481 gatggtcact ggatgacgat agtcgagcaa caccttgagg ctgttgcgcg tcagcaagtg
     9541 ctggcgctcg atcggctggt agggatcggt gttggaaccc agattgatcg gtgcgcagac
     9601 atagccgggc ttgctcagct gctgttcgag cagggccacg gcattggtct tggcgatcag
     9661 cttggtttca aaatccagcc cgggcgacag atcccaatag gcgtggctgg gcctggcata
     9721 gcagtagatg cagccatgtt cacagcctcg atagggattg agcgagcgat caaacggcaa
     9781 gtccggcgac tggttgcggg tgatgatgct tttcgcggtt tcgcagcgca cctcggtcgc
     9841 ccggctgggc ggcacctcct gaaaccagcc gtcatcgctg gcgatgatgc gctgcggggc
     9901 aaagcggtta tgcgggttac tggcggtacc gcgaccgcgc ggcggcagag aaacggacat
     9961 gggctggctc cataaattac tgtatataaa tacagtaatt tatgtgcatc accaaccccc
    10021 agtcgtcagc cgaccacctc acgcagcgcc ttaatcagcc cgccaccagc agcaataaca
    10081 gcggcaaact ggccgtagcc aacagggttt gcagggtaat gatgccggcc atcaactggc
    10141 tgtcgccgcc cagctgacgg gtcagcacgt aagccgtcgg cgcggttggc agggcaaaga
    10201 acagcaccag caccgtgctt tccatcgctg gcaactgcag accataggcc accacccaag
    10261 ccaacactgg catggccaac aggcgcagta ggctattgct aagcagcgcc ggcacttcac
    10321 cgcccagctg ctcgggtttc agtgctgcgc cgacgcagag caggccaagc ggcaggctcg
    10381 cagcggccaa caggctaagc agacgatcac tgccgcccgg caggccaagg ccgctgaggt
    10441 tgaccagcgt gccgccgagg caggcaagaa tcagcgggtt cttcagaatc ggcagcaaca
    10501 ggctgcgcgc actgacgcca cgctctgcgg tcagcgacca gaccgacagc acatttaccg
    10561 tcgggaccat cagggcgagc atgatggccg ccagggtcag gccttcctgg ccgaacaggc
    10621 tgcccaccgc cgccagcccg aggtaggtgt tgaagcgcag aatgccttgg ctgaatgcgc
    10681 caaaacgccc cgccggccaa ccccgcacgc gccgcaccag caacagcgcc aaccaggcga
    10741 tacccagcgc cagcaaaacg gctaacgcca ggcgcggcaa tgccggatta tccaacggcg
    10801 ccgtggccag gctgctgaac agcagcgcgg ggaacagaat gaagtagttc agccgctcgg
    10861 cacccggcca gaacgcctca ttggggaacg cccagcggcg caggtaatac ccggcgacga
    10921 tcaaggcaaa cagcggccac aaggccaaca acaactccag cacggcaacc tccagtcaca
    10981 ggcaagccgc catcttgagc agccgcatgg gggtgacgca aggtcgatgt cggtattagg
    11041 ctgctttacc tgacgatcac gacggggatg cccaatgtcc gatattcatc aaggtggttg
    11101 ccaatgcgga gacctgcgct atgagttcgc ggcgccactg caggacatcg cccactgtca
    11161 ttgctcgatc tgccgacgca gcacaggtgg catcgtcacc acctggatca ccgtgccgct
    11221 ggcgagcttt cgctggctgg ccggcacacc gcatgagtac gcctcgtcag ccagttgtac
    11281 gcgttatttc tgtggccagt gcggcgctca tctggcgttg ttcacccagc tcagcccgga
    11341 gaccctggat gtaaccgtcg ctacgctgga cgaacctgaa caggcgccag cggaccggca
    11401 tatctgggta ggtagccggc tgccctggct gcagctggat gagcaattgc cgcgagagga
    11461 cgaggagaac ataccttgaa ccgctagctg ccgggctagg tgccactatt ttgatgtcaa
    11521 aaatttgttt gctttgcctg tgagctatgt cacatttctg cgcctagcta tatcagggat
    11581 gttcttgtgc gttcagggat gcgcttcagg caaattcttc ttgctccatt gctcagcaaa
    11641 cccggtgctg tcctgggttt gctgctgatg ctattcgtgg cgttcttcaa cccgttcggt
    11701 ctgcgggacg cctccgaccg tcatagcgaa acctggctgt tgcgcatgct ggcgcccttc
    11761 tactccgagt ctggacagga agaggtggtg gtggtgctga ttgatgatca agcgctgatg
    11821 agcatggggg ctagctggcc gttgcgttat ggcgagcagg cgcgcctgtt gcgtctactg
    11881 ctcagctatc aaccaaaatc tctctttgtc gacctcctct acacccagag acgctacgac
    11941 ggtggcccta ccgatagcct gcagcgggca ctgggcagct tccgtgagca gaacgtgccg
    12001 ctgattcttg ccgattaccg tgatgcacaa gggcgctctc tggtattgcc cgaattcgcc
    12061 gctatggcgg acaccgcgcc aagcaactgg agcggctacg gtgagcgcta tccattgctg
    12121 ttgccaagcc cgctggggac gctgcgcaca ccggcgttgc agttatacca agcggcgtgc
    12181 caggcgcagg actgctcgcc tgaaggcttc gaggcgccgc tgctggtgcg ttggggttac
    12241 tggagcgacg caggtatgcg gggcttcgtc gacctggcag gttgtgatgt gcgtgggcag
    12301 cgggagaatt ggggacaaac attcaacatg ctggtcgccg acgctttccg ttcgctgcgc
    12361 tccgctggcg aggtcgagcg accacagccc tgtccctata cccgcaccct atatgccaac
    12421 caactgcgtg acccgagtgt cgcagaagtc ttgcgcggca agcatgtgtt gttgggtgcg
    12481 catattcgcg gcatcccgga tcttgtcagc agtccggtgc aggggctgtt gcccggcgtt
    12541 tacgtacatg caatggctct ggacaacctg ctgacgcaag ggcgcagcta ctggcgagcg
    12601 gcaccggatg gttggctggg tatcagcttg agcgactggc tagagcttgc cttgctgctg
    12661 ctggccggcc tggtcgcgct atggatcccg gcacacccgc ataccaaagg ccggttcctc
    12721 tgtcgctgtg tgagccatta cctgttctgg ttcgttctac tcagcagcct tgtattgctg
    12781 atcagtgtct atctcgcgag ctgtctgcat atcgcgccat tcgactggct gggactgctg
    12841 ctgatgatcg gcctgttcta tgcctacctt ggggaggcca gggttgccga atggtggcac
    12901 cagcgtacgc gtcgcacatc aactgaaaag gagagctagc aatgtttaga gccacagtat
    12961 tcgggttgtt gctgtgcagt gtgtcggcat gggcgcagga gccgctgaaa attatcggtt
    13021 acccgctgga aaaggtcgcg ctgtatgacg aacagggcga gttcctcggc gacataaacc
    13081 gcgaccaact gcctgttccg gatgtgcagg tgatcagttt cagcgaaacc aagaacctgt
    13141 tgctgatcgc cgttggcgag cgtcaggtct ggctcgatcc gctggatgtg aagctcaatc
    13201 acggaaagac agttgccttc gactgccgca aggtcgccga gaccagcctt ctggcagaca
    13261 gcaaaaccaa tgcggtaatg ggctattcga aagcctgcgg caagtgaatc agggaggact
    13321 catgcgtaag gaaatgacac tggcaatcgc ctgtctgctg ttgttcggtt gccagcagat
    13381 gccgtcgacc agcaaggcgc cactgcaagg caagttcatc gacagtccgc gcgcgcgccc
    13441 gctggcgctc cagccagtga ccagcaatcc ctggttgaac aacgccctgg tcgatcgcct
    13501 ggcgggcgcc gctgacccgg atgaggtgcg cgggttcggt aaggtggatc tgaccgagca
    13561 aatggcgcag gtggaaagct tccaggtgcc ggaactgcag acctattgcg atcaggtgtt
    13621 gagccgtttg ctaacacatt ggccgcatgc caaaccggac atcaaggtgc gctttagcgc
    13681 ttccgaaagc ttcggcgcgc gcgctaccgc agataatgtc atctacctca acctgggcac
    13741 cttgcaggcc atcgagagcg aggacgaact ggccgcgatg cttggacacg aagcagctca
    13801 cttgctgctg gggcacctcg atcgcgagca cttcttcaat cagcagaaga agctgctgga
    13861 tgcatcggtg acggcggtca atctgggcgt gaccctggct ggcctggaaa gccagaagag
    13921 cggcacatca cgcaaactgg tggtgggtga tcccgacgca gtgggcaaga caatcgtgca
    13981 aagcagcctc tcaggcgtcc tgctcaaggc ggcatccgat aacttctgga acagcagctg
    14041 gacgcgtgct caggaagatg aagccgatct gttgtccgtc gatctgctca gtcgtgcggg
    14101 ctactccccg cggggtgcgg tacatggcat ccagcgcctg cagagtttcg aaggcagcaa
    14161 tgtcagccag ctgcaacgct atcaggcaat gcagaaacag ctattcagcc aggcagtgca
    14221 gaaaaagggc gtcggtggct tgctcgaaca gggtatccag agcggggtga ccgctgcgca
    14281 actggcagcg gtggatctgt ggaaggattt tggcaagacc caccagttac cagctcagcg
    14341 cgaagaggcc ttagcgcgtt acatcgccag cgagtatcgc agcgagcgac gccgaagccc
    14401 tcaacttgcc agctaccagc ggcagcttct tggagggcag agcggcaaga ttctcgccgg
    14461 gcaggtgcta atcaatcgtg cgaccaaagc catcgataag ggagacctaa acgaggcacg
    14521 caagttggcc aaagacgtgg cgagagggcc ggcagccagg agcatgcgcg cacaaacctt
    14581 gcagttcgtc ctggctgttc agcaaaggcg gtacaaggaa gctctggcca atctggaaca
    14641 ggttccggac agtgagtggc agtacgcgtc tctctccagt tacgaccaga tcatcacgtt
    14701 gtcacttagc ctagaacgac aggctcaggc cgagcgctat ctgcaacgtg gccaggcgca
    14761 ttggggcaat caactgatga cgccactgga gatttccgtg cacctgcaag gcgagcgggt
    14821 cgcacaggca cagcaggcct atcagtcatg ccagagcagc acgagcaagg gcttgctggc
    14881 tcagtgcgaa tcggcgattg gtcgtttcaa accacaacag gcgcctgctg atccgctgca
    14941 gcagcttggc ctgccttcaa tgggcaagct gctcggcagc tgattcaggg cagctgcaga
    15001 ccaccgccag ccttgtgcag cgtgcgcaga tgcgtaccca gttgcgccaa gttggcttca
    15061 gtggcttgca gctcggccaa acgagccggc tccagcaggg tgcgcacttc ctggtcgagg
    15121 ctgtcgctga gctggcgcag ttgctgctgc cgcgcctggc tttcggtttc caggcgctgc
    15181 cattcggcgc gttgtggcag gccgtagcca ccttcgagca actcggccgg gcgactaagg
    15241 aaaccgctgt tggcgaggat ctgctgcagc gcgccgccgg ccttgttcaa tgtggggtca
    15301 acgccatcac ggccgctcag ataacggcgc ttgagttcat tctgagccaa gagcaattgc
    15361 cggcgcagtg ccgcctgttc gagcagtagc agcgccgcgc tggtgcgcag atcggcgcgg
    15421 gcgaaccagg gctggcgctt gtcggccgtc tgaatcagcc agtcctcaac ctgtgtctgc
    15481 tcaatcggca gctgcgtctg caacacactg aacatggcct ggtagcgatc acgaaacgag
    15541 tcgaagcgat agcccaggcg aagggcttcg gcggggtcgt tcagtacgct gcggtcagcc
    15601 agatcgcgcg ccaccagggt gtcgagcagg ccattgggca tgatgctgtc gagcgcatgc
    15661 aggcgcgggt gctggctgcc gctgcgcagc agcttgaggg tttccacggc gcagttgttg
    15721 gagataaacc agtagtcgcc gtcgtagctc cagtgctgct cggcagcgcg ggtgaccagt
    15781 tgttccagct gcgtgcggtc cagcttgagc ggcaccgagg ccaggctgcg cagttcaacc
    15841 ttggtgtatt cctcaatcac ctgctccagc ggcaaaacga acagcctcga ggggtaaacg
    15901 ccggtcaggc catcccagct ggacagctgc acatcgccaa caaaggcacg gtaggacagc
    15961 accagatgct cctgcagatc cagccggcaa tccggaccac gtggtcggcc aggcgcgcag
    16021 atgaccagac gcagcatgct gtgaccccag cggctggcca gggcatcatt ggcttcggcc
    16081 aacaggtaat ccacctgata gacccgctgc gggtcaatgc tgccgagcgg ctggcgggca
    16141 aagtcgcggc cggcatttag gtaggccaaa ccttcggcgc aaggctgctg attcgccggc
    16201 acccagccga aatgcgcccc caggtaacgc gacaaagaag gccgacgaca ggcatagctg
    16261 gggtcgagaa ggaagtactc catgttgacg gcgagaaact cgcgcgagtt gctcagctca
    16321 taactgtccg ggctacgcgc gacctgatga ttgtcctgct cgcgcgcacc acgccgcccg
    16381 acttgctgcg gccagccggc caagtcgagc agcagcggat catcactcag ggtaaaacgc
    16441 cgcgtcgtct gcccgcggca gtgatccggc aggccgacgg ctcccaggct ctgctcacgc
    16501 tggcggcact gccgttgcaa ctgccggccc accggcggcc agagctgggc acggtcatac
    16561 agatgggtaa tttcatgcag cacggtcgcc agcaattcgc ggcgcacagt gccgtggggg
    16621 cgtgaggtca gggtggtggc actgctgccg tcgcttaacc ctggcagtaa cgcggcattc
    16681 agttcgatac cgctgaaacg tccggcgcgg ccatacacct gcgcaggcag gctgcgccag
    16741 cgcacgctga cgctgcggtc gagttgttgt ttgagctgtg gcggcaaggc ggccagcgcg
    16801 tcttgcaaca gttgctggct ggcctggtgc tcagcagggc tgagttcggc gcctgcgagc
    16861 tgcaggcgca actcggcctg gctgcaggtg ctggttagta acgccagccc cagcagccag
    16921 gcacccggcc acctcacagt gccaggatgg cttcggccaa taccaggtcg gaggcgcctt
    16981 gcgcctcagg cacttggctg cgaatggtgc cgatggcggc ttccagctgc gcaccacgga
    17041 tctgcccttg gctggcgacg aaactggccg catcgtcacg cgccgcgagc acgactttcg
    17101 agtcgcgcac cgaggtggtg gtatccgagg tgaagtcgaa ggtgcgatcc agcgcacgca
    17161 ctatgatatt gctggtcgcc accagggttt gcgcctgtgt agcggcactg aacagaacaa
    17221 ctgcaactac ggcactgagc aagatagggc gcatatgcat ctccaataat cagccatcaa
    17281 gtcgtttgga ctggcttaga cgggcagtgt gccacacagt tccgccagcg cttctggcta
    17341 agcgctggca gcacacatat tcagaggcta aggatggccg ttgccagctg catatcgtcg
    17401 ccttgcaacg tcggtacctg ctgacggata tgctgtagag cggcctccag gcgtacccca
    17461 cggatgtcgc cctggctggc aacaaacgcg gcagcgtcat cgcgcgcggc cagcacgacc
    17521 ttgtcatcgg taaaggatga ggacacatct gagctcgctt cagcggttgc cgcgaccgcc
    17581 cctaccaccg catcagtggt gaccacaaaa ctggtggcgc ttgccgtacc ggccagcgcc
    17641 agtaatgccg caaggctcaa agtctttatc gcattcatat ccacactcct aaaaaggccc
    17701 aacaggcagg ctaggttcag tgtagacccc ggctgtatgg gttggttgcc cggctgctta
    17761 acgccagaac ggctttgcca actcggcctg acgctgcgcc tcggtgatgc ctgcgtccgc
    17821 cagctggcgg ctatcgaggc gcgccagttg gtggcgggta cgcaggttgc gctgccagcc
    17881 acagaacgcg gcataaacac gtaaccagag cggggaattt acttgagtat ttaccggggg
    17941 ctggtgcttg agagtgcgat ccatggcgca attccttgtc ggagacggaa gaaggcaacc
    18001 tatggtgcgc gggagggagc ggtaggtgca gagacagcac agggaaattg ttatggcgca
    18061 gttagctttt tacaacaact gtaccgctta acagcgcagc agactgttgc agccaagcaa
    18121 ttgcctgagt acttttcccg gcacataaaa agaaagcccc aacacgcagg gcgctggagc
    18181 tttcaaaaat cccataaagg gaggagcaga aatcggtgtc tggcagcagt taacgccaga
    18241 aaggcttgct cagttcagcg tgacgctgtg cttcgctgat accggcgtct gcgagcagac
    18301 gggagtccag acgagcaagc tggcggcggc taacaacgcg gcgctgccat tgcagaacag
    18361 aggcgagcat acgcagcggc agagcgctgg agccggtgga ttggctgtga tcgaaaaaca
    18421 ggtcggaact gagggtacgt tccatggtgg tcatccttcc gcttgtggcg ggtcagtggt
    18481 gtgttgcgat gggagaatta ttgccgttgc ccccacttcg ctgtagccac agctgaatga
    18541 aattgcagca caaa
//
LOCUS       DASRUM010000240         3748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_461225, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000240 DASRUM010000000
VERSION     DASRUM010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461225"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1615
                     /gene="gshA"
                     /locus_tag="VFF22_16005"
     CDS             35..1615
                     /gene="gshA"
                     /locus_tag="VFF22_16005"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HZX18356.1"
                     /translation="MSDLLPRRLAQLGERANLSLLSQCLHGIERECLRVDGDGQLALT
                     AHPAALGSALTHTQITTDYSESLLEFITPAEPDPAATLADLEQIHRFAYSKLDGEYLW
                     SPSMPCALPDEETIPIARYGNSHIGELKYVYRKGLALRYGKTMQCIAGIHYNFSLPDA
                     LWQLQQQAEGDTRSAQDYQSARYIALIRNFRRYSWLLMYLFGASPALHKGFMRGRPHQ
                     LQELDGETLYLPYATSLRMSDLGYQSTAQSGLTPCYNDLTSYTDSLRQAVGTPYPPYV
                     EIGAKKDGQWLQLNTNILQIENEYYSNIRPKRVTYSGERPIQALMARGVQYVEVRCLD
                     INPFLPLGIDLTESRFLDAFLLFCALADSPLLESGECHACTDNFLAVVKEGRRPGLQL
                     QRNGQPVVLQAWADELLEQIRPLAELLDQNHDSNAHRLALQAQQAKVADASLTPSAQV
                     LAQLQQGLSFTEFALRQSKLHAEYFRSQPLSAEQQAAFEASAAQSLQDQAELEAQEEG
                     DFDSFAAAYQASILALAV"
     gene            1765..1974
                     /locus_tag="VFF22_16010"
     CDS             1765..1974
                     /locus_tag="VFF22_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HZX18357.1"
                     /translation="MACADLNTEDDQHWSLESLNKAYQQGYMAGLTGQANTPRGLAAD
                     VLIAAWEAGWDDGHEQFELHKRYSA"
     gene            2276..3157
                     /gene="aguB"
                     /locus_tag="VFF22_16015"
     CDS             2276..3157
                     /gene="aguB"
                     /locus_tag="VFF22_16015"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HZX18358.1"
                     /translation="MPRIVTVAATQMACSWDTAANLANAERLVRQAAGKGAQIILLQE
                     LFEAPYFCQKPNPDYLQLATPAESNPAILHFQKIAKELQVVLPISFYELAGRARFNSI
                     AIIDADGSNLGIYRKSHIPDGPGYHEKYYFNPGDTGFKVWNTRYAKIGVGICWDQWFP
                     ECARSMALLGAEILFYPTAIGSEPHDPSISSRDHWQRVQQGHAGANLMPLIASNRIGR
                     EDQGDYHINFYGSSFIADQFGAKVQEANETDEAVLMHSFDLDQLEHIRSAWGSFRDRR
                     PNLYGTLKTLDGHLTSE"
     gene            3233..>3748
                     /locus_tag="VFF22_16020"
     CDS             3233..>3748
                     /locus_tag="VFF22_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HZX18359.1"
                     /translation="MTTLTTTPHADGFRMPAEWESHSQTWMVWPERPDNWRLGGKPAQ
                     AAFTAVARAIAEFEPVTVCVSAGQYENACERLAHANIRVVEITTDDAWVRDTGPTFVT
                     NNSGEVRGVDWAFNAWGGLNGGLYANWVRDDQVARKILQIEGCARYRTDELILEGGSI
                     HVDGEGTLITTE"
BASE COUNT          769 a         1280 c         1061 g          638 t
ORIGIN      
        1 tattgaggcg accgatgcgt gaaggaatgc aatcttgagc gacctgttac cccgccgcct
       61 ggcccagctt ggcgagcgcg ccaacctctc cctgctttcc caatgcctgc acggtatcga
      121 gcgcgaatgc ctgcgcgtcg atggcgacgg ccagctggcg ctgactgcgc acccggccgc
      181 cctcggctcg gcgctgaccc acacgcagat caccaccgac tactcagaat cgctgctgga
      241 atttatcacc ccagccgagc ctgacccggc agcgaccctg gccgatctgg agcagatcca
      301 ccgtttcgcc tacagcaagc tggatggcga gtacctgtgg agcccgtcga tgccctgcgc
      361 cctgccggac gaagagacaa tcccgatcgc ccgttacggc aactcgcata tcggcgaact
      421 caagtacgtg taccgcaagg gtctggccct gcgttacggc aagaccatgc aatgcatcgc
      481 cggcatccac tacaacttct ccctgcccga cgcgctctgg cagctgcagc agcaggccga
      541 aggcgatacg cgtagtgcgc aggattacca gtcggcgcgc tatatcgcgc tgatccgcaa
      601 tttccgccgt tacagctggc tgctgatgta cctgttcggt gcctcgccgg cgctgcacaa
      661 gggctttatg cgcggccgcc cgcaccagct gcaggagctg gatggcgaaa ccctttacct
      721 gccttatgcc accagcctgc gcatgagcga cctgggctac cagagcaccg cccaatccgg
      781 cctgacgccc tgctacaacg acctgaccag ctacaccgac agcctgcgcc aggctgtcgg
      841 cacgccctac ccgccctatg tggaaattgg cgcgaagaag gatggtcagt ggctacagtt
      901 gaacaccaac atcctgcaga tcgagaacga gtactactcc aacatccgcc ccaagcgcgt
      961 gacctatagc ggtgagcggc cgatccaggc gctgatggcc cgcggcgtgc agtacgtcga
     1021 agtacgctgc ctggacatca acccgttcct gccgctgggc atcgacctaa ccgaatcgcg
     1081 cttcctcgac gccttcctgc tgttctgcgc gctggccgac agtccgctgc tggaaagcgg
     1141 cgaatgccat gcctgcaccg acaacttcct ggccgtggtc aaggaaggtc gtcgtccggg
     1201 cctacagctg caacgcaatg gccaaccggt ggtgctgcag gcctgggccg atgagttgct
     1261 cgaacagatc cgtccgctgg ccgagctgct cgatcagaac cacgacagca acgcccaccg
     1321 cctggccttg caggcgcaac aggccaaggt cgccgatgcc agcctgacgc cctccgccca
     1381 ggtgctggcg caactgcaac agggcctgag ctttaccgag ttcgccctgc gccagagcaa
     1441 gctgcacgcc gagtacttcc gcagccagcc gctaagcgcc gaacagcaag cagccttcga
     1501 ggccagcgcc gcccagtcgc tgcaggacca agccgaactt gaagcccagg aagaaggcga
     1561 cttcgacagc tttgccgccg cctatcaggc cagcattctg gcgttagctg tttaactccg
     1621 aagaatgccg gctcgcgcgc agccggcatt tcgccaactg catcacataa accgccagcg
     1681 gtggcaaaag cgggatcagc ggcgcagccc tgcccaggcg atgcggctag cattgcatgg
     1741 gtaaatccac tgcgagctgc cgccatggcc tgcgccgacc tcaacacgga agatgaccag
     1801 cactggagcc tggaaagcct caacaaggct taccaacaag gctatatggc agggctgacc
     1861 ggacaagcca acaccccacg cgggctcgcc gccgatgtgc tgatcgccgc ctgggaagcc
     1921 ggctgggacg acggccacga gcaattcgag ctgcacaaac gctacagcgc ctgatccctc
     1981 gccttaccca accctcacct gcccacaccg ttaacacccg cagcactcag cctgcgtggc
     2041 ggcgtatcgc cttgcctttg atcgaccttc attttgccgg cgcaaactaa ttcgattttt
     2101 attcgatata aatcggtaaa aattcgttta gcgccgtatg ttttgctttt aaagccgatt
     2161 tttatcggat ataaataggc catccactgc catgactcac ccgcgaggca tggcacagtc
     2221 gccggcctct gctagtgtca gatgaccgcc ccaactgctc ttgaggtgac aaaccatgcc
     2281 ccgtatcgtt accgtcgccg ccacccagat ggcctgctcc tgggataccg ctgcgaatct
     2341 cgccaacgcc gagcgcttgg tgcgtcaggc cgccggcaag ggcgcgcaga tcatcctgct
     2401 gcaagagctg ttcgaggccc cgtacttctg ccagaagccc aacccggact atctgcagct
     2461 ggccacccca gccgagagta acccggcgat tctgcacttc cagaaaatcg ccaaagagct
     2521 gcaagtggta ctgcccatca gcttctatga actggccggc cgcgcgcgct tcaacagcat
     2581 cgcgatcatc gacgccgacg gcagcaacct cgggatttat cggaaaagcc atatcccgga
     2641 cggcccgggc taccacgaga agtactactt caaccccggc gacaccggct tcaaagtgtg
     2701 gaacacccgc tacgccaaga tcggcgtggg catctgctgg gaccagtggt tccccgagtg
     2761 cgcgcgcagc atggcgctgc tcggtgccga gattctgttc tacccgaccg ccatcggcag
     2821 cgagccgcat gacccgagca tcagctcgcg cgaccattgg cagcgcgtac agcagggcca
     2881 cgccggcgcc aacctgatgc cgctgatcgc cagcaaccgc atcggtcgtg aagaccaggg
     2941 cgattatcac atcaacttct atggctcctc gtttattgcc gaccagttcg gcgccaaggt
     3001 gcaggaagcc aacgaaaccg acgaagcggt gctgatgcac agcttcgacc tggaccagct
     3061 agagcatatc cgcagcgcct ggggcagctt ccgtgaccgc cgcccgaacc tctacggcac
     3121 gctaaaaacc ctcgacggcc atctgacctc cgagtaaaaa cgcccctgtt aagatgggtt
     3181 gtgacgcgca gcgttaatgc ccatccgccc cgctcaatga tttggtgaat ttatgacaac
     3241 cctgacgact accccgcacg ccgacggctt ccgcatgccc gccgagtggg aaagccacag
     3301 ccaaacctgg atggtctggc ccgagcgccc ggacaattgg cgcctgggcg gcaagcccgc
     3361 gcaagccgcg tttaccgccg tggccagggc gattgccgaa ttcgaaccgg tgactgtgtg
     3421 cgtgtccgcc ggccagtatg agaacgcctg cgagcgcctg gcccacgcca atatccgcgt
     3481 ggtggaaatc accaccgacg acgcctgggt gcgcgacacc gggccgacct tcgtcaccaa
     3541 caacagtggc gaagtgcgcg gtgtggactg ggcgttcaac gcctggggcg gcctgaatgg
     3601 cggcctgtac gccaactggg tgcgtgacga tcaggtggcg cgcaagattc tgcagatcga
     3661 aggctgcgcg cgttatcgca ccgatgagct gatcctcgaa ggcggctcga tccatgtcga
     3721 cggcgaaggc accctgatca ccaccgag
//
LOCUS       DASRUM010000241         6309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_462800, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000241 DASRUM010000000
VERSION     DASRUM010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462800"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1387
                     /gene="pepP"
                     /locus_tag="VFF22_16025"
     CDS             53..1387
                     /gene="pepP"
                     /locus_tag="VFF22_16025"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HZX18360.1"
                     /translation="MISIPKSEYARRRKALMAQMEPNSIAILPAAPVYIRNRDVEHIY
                     RQDSDFQYLSGFPEPEAVIALIPGREHGEYVLFCRERDPLREMWDGLRAGQDGAISQY
                     GADDAFPIGDIDDILPGLIEGRDRVYSSMGTNHEFDRQLMEWINVIRSKARQGASPPN
                     EFITLEHLLHDLRLYKSAAEVKVMKEAADISCRAHVRAMQTSRAGLFEYHLEAELDYE
                     FRKGGAKMPAYGSIVAAGKNACILHYRENDAPLKDGDLVLIDAGCEIDCYASDITRTF
                     PVSGKFSPEQKAIYELVLASQEAAFKEIGPGKHWNQAHEATVQVITAGLVELGLLQGD
                     VAELIATEAYKPFYMHRAGHWLGMDVHDVGEYKVGGEWRELEVGMAMTVEPGIYIAVD
                     NQDVAKKWRGIGVRIEDDVVVTKTGCEILTSGVPKTVAEIEALMAAARSVAA"
     gene            1391..2578
                     /gene="ubiH"
                     /locus_tag="VFF22_16030"
                     /gene_synonym="visB"
     CDS             1391..2578
                     /gene="ubiH"
                     /locus_tag="VFF22_16030"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019817789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HZX18361.1"
                     /translation="MTRVDIAIIGGGLVGASLAVALQAEAKLRGWSICLIEPFTPGNG
                     YQPSYDARSTALSFGSQQIYQRLGLWQSISQRAEPIKQIHVSDRGRFAAARLSAEEEG
                     VPALGYVVENAWLGECLWQALDQQVVSWRSPAQVTRMQALADGYRLTLDDGNELECNL
                     AILAEGGRSGLREQLGIAVSTTPYGQSALIANVSPQEAHNGLAFERFTEEGPMALLPL
                     ADNRCALVWTRATLEAERLSALDDAAFLGELQQAFGYRLGSLRQVGARHLYPLALTAA
                     KEQVRQHLVVLGNAAHSLHPIAGQGYNLSLRDTLVLAETLLVSSAPLGDLATLQRYLS
                     SQQMDQQLTVGFSDQVTRLFSTGQPLLAAGRNLGLLGLDLLPPAKRWFARQAMGLGTR
                     SEH"
     gene            2582..3064
                     /locus_tag="VFF22_16035"
     CDS             2582..3064
                     /locus_tag="VFF22_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HZX18362.1"
                     /translation="MLANKLARKARLLRWAMNLYPPYLGAGVRVRQISADFRQVQVKM
                     GLGWYNRNYVGTQFGGSLYSMTDPFFMLMIMENLGRDFIVWDKAARIDFIAPGTGPVY
                     ADFSIDQGLLDDIRQHTADGDKYHPELHVEVRDGSGTLVARVHKTLYVRLKPRARQAA
                     "
     gene            3079..4296
                     /locus_tag="VFF22_16040"
     CDS             3079..4296
                     /locus_tag="VFF22_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HZX18363.1"
                     /translation="MQADLIIVGAGMVGSALALALQDQGLEILLVDGGPLSVKPFDPQ
                     AAFEPRVSALSVASQRILDRLGVWDGIAARRACPYGEMQVWDGSGTGQIHFSAASVHA
                     DSLGHIVENRVVQDALLERLHDSPIGLLPGARLEQLRRSGDGWLLSLSDGRELRAPLL
                     IAADGANSAVRRLAGCATREWDYLHHAIVTSVRCAKPHQATAWQRFTDDGPLAFLPLA
                     GPEGEHWCSIVWSVTPTEAERLMALDDAGFRRALGFAFEHRLGDILQADPRLCIPLRQ
                     RHAKRYVEDGLALIGDAAHSIHPLAGQGVNLGFLDVAVLAEVLLHAAQRGERLGDVKV
                     LSRYERRRMPHNLAMMAAMEGFERLFQADPLPVRWLRNSGLNWVDDLPEAKALFVRQA
                     LGLSGDLPELARV"
     gene            complement(4487..5770)
                     /gene="dctM"
                     /locus_tag="VFF22_16045"
     CDS             complement(4487..5770)
                     /gene="dctM"
                     /locus_tag="VFF22_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter large permease
                     protein DctM"
                     /protein_id="HZX18364.1"
                     /translation="MTILFLFVLLFLLMFIGVPIAASLGLAGSVTIMLFSPDSVRSLA
                     IKLFETSEHYTLLAIPFFLLSGAFMTTGGVAKRLIDFANACVGHIRGGLAISAVLACM
                     LFAALSGSSPATVAAVGSIAIAGMVRSGYPQAFGAGIVCNAGTLGILIPPSIVMVVYA
                     AATEQSVGKLFMAGVVPGLLLGVALMVAIYIVARKMNLPAMPRASFREFITAARKAVW
                     GLLLMVIILGGIYTGMFTPTEAAAVAAVYAGFVALFVYKDMTIRECPKVLLESGKLTI
                     MLMFIIANAMLFAHVLTTEQIPQTITAWVVDMGLQPWQFLLVVNIVLLIAGAFMEPSA
                     IILILAPILFPIAVQLGIDPIHLGIIMVVNMEIGLITPPVGLNLFVTSAVTGMPLTAV
                     VKAAWPWLMLLLGFLMVITYIPAVSMALPNWLGMS"
     gene            complement(5767..>6309)
                     /locus_tag="VFF22_16050"
     CDS             complement(5767..>6309)
                     /locus_tag="VFF22_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003197903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HZX18365.1"
                     /translation="VILNNLYTLFYNLADRWEGSSEFFFAIGDSILGMAQSMTWSIAL
                     TKALFAWLIFFGLAYGVRTAGHIGVDALVKLASKPVQRAIGLIACLACLGYAGLITVA
                     SFDWVKTLFTAGIGAEDLGHYGIMQWHITLIVPFGYAMVFIRFFEIFVRILRNQQTGL
                     GLADEAAEAMKLTEHEEHKP"
BASE COUNT         1219 a         1892 c         2036 g         1162 t
ORIGIN      
        1 gaagccgtcg ctgcattgat tttcatgcaa tagaagtgga ctcaatctgc ccatgatcag
       61 catccccaag tcggaatacg cccgtcggcg caaggcgctg atggcgcaga tggaacccaa
      121 cagcatcgcg attctgccgg ctgcgccggt gtatatccgc aaccgcgatg ttgagcatat
      181 ctaccgtcag gacagcgact ttcagtacct ctccggcttt cccgagccgg aggcggtaat
      241 tgcgctgatt cccgggcgcg agcatggcga gtacgtattg ttctgccgtg agcgcgaccc
      301 gttgcgggaa atgtgggacg gcttgcgcgc aggccaggat ggtgcgatca gccagtacgg
      361 cgcggatgat gcgttcccga ttggtgatat cgacgacatc ctgccggggc tgatcgaagg
      421 gcgtgatcgg gtctattcct cgatgggcac taaccatgag ttcgaccgcc agctgatgga
      481 gtggatcaac gtgatccgct ccaaggcgcg ccagggtgcc tcgccgccta acgaattcat
      541 caccctcgaa cacttgctgc atgacctgcg gctgtacaag tcggcggccg aggtcaaagt
      601 catgaaggaa gcggcggaca tctcctgccg cgcccatgtg cgcgccatgc agaccagtcg
      661 tgctgggttg ttcgagtacc acctggaagc cgagctggat tacgagttcc gcaagggcgg
      721 ggcgaagatg ccggcctatg gttccatcgt cgctgccggc aagaacgcct gcatcctgca
      781 ttaccgcgag aacgacgcgc cgctgaaaga cggcgatctg gtgctgatcg acgccggttg
      841 cgaaatcgac tgctacgcca gcgacatcac ccgcaccttc ccggtcagcg gcaagttttc
      901 gccagaacag aaagccatct acgagctggt gctggcttcg caggaagccg cgttcaagga
      961 aatcggcccc ggcaagcact ggaatcaggc ccacgaagcc acggtgcagg taattaccgc
     1021 cgggctggtg gagctcggcc tgctgcaggg cgatgtggcc gagctgatcg ccactgaggc
     1081 ctacaaaccc ttctatatgc accgcgccgg ccactggctg ggcatggatg tgcacgatgt
     1141 cggcgagtac aaggtcggcg gcgaatggcg cgagcttgaa gtcggcatgg ccatgacggt
     1201 tgagccgggc atctatatcg ccgtggacaa tcaggatgtg gcgaagaagt ggcgcggcat
     1261 cggtgtgcgc atcgaggacg acgtggtggt gaccaagact ggctgcgaga ttctcaccag
     1321 cggcgtgccg aaaaccgtcg ccgagatcga agcactgatg gccgccgcac gcagcgtggc
     1381 tgcctagagc atgacacgcg tcgacattgc gattatcggc ggcggcttgg taggcgctag
     1441 ccttgcggtg gctttgcagg ccgaggccaa gctgcgcggc tggagcattt gcctgattga
     1501 gccgttcacc ccaggcaatg gttatcagcc cagctacgac gcgcgctcga ctgcattgtc
     1561 gtttggctcg cagcagatct atcaacgcct cggtctatgg cagtcgatca gccagcgtgc
     1621 cgagccgatc aagcagattc atgtctccga tcgtggccgt ttcgccgcag cgcgcctgag
     1681 cgccgaggaa gagggcgtgc cggcactcgg ttatgtggtg gaaaacgcct ggctcggcga
     1741 atgcctgtgg caggcgctgg atcagcaggt ggtgagctgg cgcagcccgg cgcaggtcac
     1801 gcggatgcag gccttggccg atggctatcg cctgaccctg gacgacggca acgagctgga
     1861 atgcaacctg gcgattctcg ccgaaggcgg ccgctccggt ttgcgcgagc aactgggtat
     1921 cgccgtcagc actacgccgt atgggcagag cgcacttatc gccaatgtca gcccgcagga
     1981 agcccataac ggcctggcct ttgagcgctt taccgaagaa ggaccgatgg ccctgctgcc
     2041 gctggcggat aaccgctgcg ccctggtctg gacccgtgcc accctggaag ccgagcggct
     2101 gtcggcgctg gacgatgcgg cgtttctcgg cgagctgcag caggccttcg gttatcgcct
     2161 gggcagcctg cgtcaggtcg gcgcgcgcca tctctatccg ctggcactga ctgcagccaa
     2221 ggagcaggtg cgtcagcatc tggtggtgct cggtaatgcg gcgcacagct tgcatccgat
     2281 tgccgggcag ggctacaacc tgtcgctgcg cgataccctg gtgctggccg aaaccctgct
     2341 ggtcagcagc gcgccgttgg gcgatctggc caccctgcag cgttatctga gcagccagca
     2401 gatggatcag cagttgaccg tcggcttctc cgatcaggtc acccggttgt ttagcaccgg
     2461 ccagccgctg ttggctgctg ggcgcaacct tggcctgctc ggcctggatc tattacctcc
     2521 ggcgaaacgc tggttcgccc gtcaggccat gggccttggc actcgctcgg agcattgatt
     2581 catgttggca aacaaattgg cgcgcaaggc gcggctgctg cgctgggcga tgaatctgta
     2641 tccgccgtat ctgggcgccg gcgtgcgggt gcggcagatc agcgcggact tccgtcaggt
     2701 gcaggtgaag atgggcctgg gctggtataa ccgcaactat gtcggcaccc agttcggcgg
     2761 cagcctgtat tcgatgaccg acccattttt catgctgatg atcatggaaa acctggggcg
     2821 tgattttatc gtctgggaca aggccgcgcg catcgacttt attgcccccg gcaccggccc
     2881 ggtgtacgcc gatttcagca tcgaccaagg gctgcttgac gacattcgtc agcacacggc
     2941 agatggcgat aagtaccacc ccgagctgca tgtggaggtg cgcgatggta gcggcaccct
     3001 ggtggcgcgg gtgcacaaga ccctttatgt gcggttgaaa ccgcgcgctc ggcaggcagc
     3061 ctgaacaagg aatatgtgat gcaagcggat ctgatcatcg taggcgcagg catggtaggc
     3121 agcgccctgg cgctggccct gcaggatcag gggctggaga ttctgctggt cgatggcggc
     3181 ccgctgagcg tcaagccttt tgatccgcag gctgccttcg aaccgcgtgt cagcgcactg
     3241 tcagttgcca gtcagcgcat tctcgatcgc ctgggtgtgt gggacggcat cgccgcgcgg
     3301 cgcgcctgcc cctatggcga gatgcaggtg tgggacggct ctggcaccgg gcagattcat
     3361 ttcagcgcgg ccagcgtgca tgccgactcc ctcggccata tcgtcgagaa ccgcgtggtg
     3421 caggatgccc tgcttgaacg tctgcacgac agtccgattg gtctgttgcc gggcgctcgc
     3481 cttgagcagc tgcgccgctc cggcgatggc tggctgctca gtttgagtga tggccgcgaa
     3541 ctgcgtgcgc cgctgctgat tgccgccgac ggcgccaact cggcggtgcg ccgcttggcc
     3601 ggttgcgcca cccgcgagtg ggattacctg caccatgcca ttgtcaccag tgtgcgctgc
     3661 gccaagccgc accaggccac cgcctggcag cgctttaccg atgacggtcc gctggccttt
     3721 ctgccgctgg caggtcccga aggtgagcat tggtgctcga ttgtctggtc tgttacccca
     3781 actgaagccg aacgactgat ggcgctggat gatgccggtt tccgtcgtgc gctgggcttt
     3841 gccttcgagc atcgtctggg cgatatcctc caggccgatc cgcgcctgtg tattccgctg
     3901 cgtcagcgcc acgccaagcg ctatgttgaa gatggtctgg cgctgatcgg cgatgccgcg
     3961 cacagcatcc acccgctggc cggccagggc gtcaacttgg gctttctcga tgtcgcagtg
     4021 ctggccgagg tgctgctgca tgccgcgcaa cgtggcgaac gtttgggtga tgtgaaggtg
     4081 ctcagccgtt acgagcgccg gcgcatgccg cacaatctgg cgatgatggc ggcgatggaa
     4141 ggcttcgagc ggctgttcca ggccgacccg ctgcccgtac gctggctgcg caatagcggc
     4201 ctcaactggg tggatgatct gccggaagcc aaggcgctgt ttgtgcgtca ggcgttgggc
     4261 ttgtccggcg atctgccgga gttggcgcgg gtttaactat ctagcccagt gccgtgcgtt
     4321 acgccgatga tgggttacgc cgcgtttcgc gcggcgtgat acctgccttt gcggcatgcg
     4381 gctaacctgc gcggcccgac ccaccctaca aaaactacag gcaaaaaaag cccggcacta
     4441 aggccgggct gaaaaagtgt gtagggaagt acacacggag ttgctgtcag ctcatgccca
     4501 gccagttcgg caaggccatg gatacggccg ggatgtaggt gatcaccatc aggaagccga
     4561 gaagcagcat cagccacggc caggcggcct tgaccacggc ggtgagcggc atgccggtca
     4621 ccgctgaggt gacgaacagg ttcagcccca ccggtggtgt gatcaatccg atttccatgt
     4681 tcaccaccat gatgatgccc aggtggatcg ggtcgatgcc cagttgcacg gcaatcggga
     4741 agaggatagg cgcgaggatc aggatgatcg ccgatggctc cataaaggca ccggcgatca
     4801 gcagcacgat gttcaccacc agcaggaact gccaaggctg cagacccata tccactaccc
     4861 aggcggtgat ggtctgcggg atctgttcgg tggtcagcac gtgggcgaag agcatggcgt
     4921 tggcgatgat gaacatcaac atgatggtca gcttgccgga ctccagcagc accttggggc
     4981 actcgcggat ggtcatgtcc ttgtacacga acagcgccac gaagccggcg tagaccgccg
     5041 ccaccgctgc tgcttcggtc ggggtgaaca tgccggtgta gataccgccg aggatgatca
     5101 ccatcagcag caggccccat acggctttgc gtgcagcggt gatgaactca cggaagctgg
     5161 cgcgcggcat ggccggcaga ttcatcttcc gcgcgacgat atagatcgcc accatcagtg
     5221 ccacgcccag cagcaggccc ggtacgacac cagccataaa cagcttgccc accgactgct
     5281 cggtggcagc ggcgtacacc accataacga ttgacggcgg aatcaggatg cccagggtac
     5341 cggcgttaca cacgataccg gcgccaaacg cctgcggata acccgagcgc accatgccgg
     5401 cgatggcaat cgaacccacc gcagccacgg tggctggtga ggaaccggac agcgcggcga
     5461 acagcatgca cgccaatacc gccgagattg ccaggccgcc acggatatgg ccgacgcagg
     5521 cgttggcaaa gtcgatcagg cgcttggcca cgccgccagt ggtcataaat gcaccggaca
     5581 gcaggaagaa cggaatcgcc aacagggtgt agtgctcgct ggtctcaaac agcttgatcg
     5641 ccagcgaacg caccgagtcc gggctgaaca gcatgatcgt caccgagccg gccaggccca
     5701 gcgaggcggc aatcggcacg ccgataaaca tcagcagaaa cagcaggaca aagaggaaca
     5761 ggatggtcat ggtttgtgct cctcatgctc ggtcaacttc atcgcttcgg cagcctcatc
     5821 ggccaggccg agaccggtct gctggttacg cagaatgcgc acgaaaattt caaagaagcg
     5881 gataaacacc atggcgtagc cgaatggcac gatcagggtg atatgccatt gcatgatgcc
     5941 gtagtgaccg aggtcttcag caccgatgcc ggcggtgaac agggttttca cccagtcgaa
     6001 gctggccacg gtgatcagtc cggcatagcc caggcaggcc aggcaagcga tcaggccgat
     6061 agcccgttgc accggtttgc tcgccagttt caccagcgca tcgacgccga tatgccccgc
     6121 ggtacgcaca ccgtaggcca ggccgaagaa aatcaaccag gcgaataacg ccttggtcag
     6181 ggcaatgctc caggtcatcg actgggccat gccgagaatg ctgtcgccaa tggcgaagaa
     6241 aaactcactg ctgccttccc agcgatccgc caggttgtag aacagtgtgt aaaggttatt
     6301 gaggatcac
//
LOCUS       DASRUM010000242        13286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2142865, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000242 DASRUM010000000
VERSION     DASRUM010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 13286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13286
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2142865"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..1528
                     /gene="purF"
                     /locus_tag="VFF22_16055"
     CDS             20..1528
                     /gene="purF"
                     /locus_tag="VFF22_16055"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZX18366.1"
                     /translation="MCGIVGIVGKSNVNQALYDGLTVLQHRGQDAAGIVTSHDGRLFL
                     RKDNGLVRDVFQQRHMQRLVGHMGIGHVRYPTAGSSSSAEAQPFYVNSPYGITLAHNG
                     NLTNVEQLAKEIYESDLRHVNTNSDSEVLLNVFAHELAQRGKLQPTEEDVFAAVTDVH
                     ERCLGGYAVVAMITGYGIVGFRDPNGIRPIVFGQRHTDEGVEYMIASESVSLDVLGFT
                     LIRDLAPGEAVYITEEGKLFTRQCASNPKYAPCIFEHVYLARPDSIMDGISVYKARLR
                     MGEKLADKILRERPQHDIDVVIPIPDTSRTAALELANHLGVKFREGFVKNRYIGRTFI
                     MPGQAARKKSVRQKLNAIELEFRGKNVMLVDDSIVRGTTCKQIIQMAREAGAKNVYFC
                     SAAPAVRYPNVYGIDMPSAHELIAHGRTTEEVCELIGADWLIYQDLPDLIEAVSGSKK
                     VKIDNFDCAVFDGKYVTGDIDEAYLDKIEQARNDASKVKSQAVSAIIDLYNN"
     gene            1574..2785
                     /locus_tag="VFF22_16060"
     CDS             1574..2785
                     /locus_tag="VFF22_16060"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HZX18367.1"
                     /translation="MTIEWQAGRLDSDLDGVGFDTLAVRAGQHRSPEGEHSEAIYPTS
                     SYVFRTAADAAARFAGEVPGNVYSRYTNPTVRAFEERIAALEGAEQAVATSSGMSAIL
                     AIVMSLCSAGDHVLVSRSVFGSTISLFEKYLKRFGVQVDYVPLADLDGWAAAFKPNTK
                     LLFVESPSNPLAELVDIAALAEIAHSKGALLAVDNCFCTPALQQPLKLGADVVMHSAT
                     KYIDGQGRSMGGVVAGSAKLMTEVVGFLRTAGPTLSPFNAWIFLKGLETLRIRMQAHC
                     ASALQLAQWLEQQPGIEHVYYAGLPSHPQHELAKRQQSAFGAVVSFEIKGGKEAAWRF
                     IDATRVISITTNLGDTKTTIAHPATTSHGRLSPAERANAGIRDNLIRVAVGLEDIADL
                     QADLARGLAAL"
     gene            3015..5654
                     /locus_tag="VFF22_16065"
     CDS             3015..5654
                     /locus_tag="VFF22_16065"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZX18368.1"
                     /translation="MDRLAPCVLPANGVLRQSLLELLARSEDFALTLLLAPAGSGKST
                     LLQHWRNRCEAALVHYPLQARDNDPLCFFRRLAESIRAQVGDFDTSWFNPLALTSSLS
                     PQLLGDLLGDALARVEGPLFIVLDDFQWIDSCSILEVMAALMERVPAHVHLIIASRNH
                     PGFALSQLKLENRLLCIDQHDLRLSAEQVQQLNSCLGGQALSQAYVDSLLGMTEGWVA
                     GVKIALLAYARFGTQALQRFNGSQPEIVDYFGHVVLRQLPPNFREFLLCSGLFERFDG
                     SLCDHVLQCTGSALLLEALAERQLFLLPVENQPGWFRFHALLQDFLCRRLQVEEYARL
                     DQLHSRAADYYLSLGEHEQALQHAQRCKDQALFLDMLESSCASWVRSGQFGDILRWLE
                     PLSEALLLAQPKLLGWLIAALSLSRRFHQAHYHLELLDSLERSAPGANLEPATRQFLA
                     LLLGVFEQDKDFMPPSAWRDLLGTVQNLEVRACTLAVIAYQHLLAARLQDAIRFASES
                     KQLLAQCGYSFLESYTDLIIALCHRNAGRATHARKQVCLDYQATDPASPAWVNRATAM
                     VVVLYEQNQLVEAQQLCDELMAMVNSSSATEAIATVYITLSRLQHRQQQTARASRLLD
                     QLWCILQLGNYARFVSQVAQEGMRQAYLSGKHAAMDAVAQRFDLPARLQAGEWESVRT
                     YDESWERLGLACVYWLQARGGQAQAERILKVLAASLRQSEMRARLLIVEANRLVLAAS
                     SQSKSQRLAALGGLVETYGIVNINRSVFDEAPGFGAGVLSLAQAELLNVPEKYREHYG
                     EFLQAPQLLPQTSALSTSLLTGREVEVFECLLSGLSNTQISDKTGIALSTTKWHLKNI
                     YSKLNVSSRTEAILAVRPRVSLN"
     gene            5793..8537
                     /locus_tag="VFF22_16070"
     CDS             5793..8537
                     /locus_tag="VFF22_16070"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HZX18369.1"
                     /translation="MSVWVTWPALTKLGTLGVVSGLLVLAVERQALFENNLFDVENYA
                     GYNADITCDARSLAARTEDGTCNILSNPAEGSVYRRFGRNVNPAVTQGETTTLLSPNP
                     REVSNSLMAREEFKPAPSLNFIAAAWIQFMVHDWFDHGPNADADPIQFPLPPGDVLGS
                     GSMSVKRTQPDPTRSSADAGKPQTYRNHNTHWWDGSQLYGSSKETNDKVRSFVDGKLK
                     VNANGTLPTELVSGKPVTGFSENWWLGLSMLHQLFTLEHNAIASRLKQKYPAQSDQWL
                     YDRARLINSALMAKIHTVEWTPAVIANPITERAMYANWWGLLGQGGPRDDYQAEVRML
                     QEDLAKSDSFVKRILGFDPNVAPGVGNSAFDHAFSGIVGSTAPNNYGVPFTLTEEFVA
                     VYRMHPLMRDNVQVYDIGSNQVSRTIALQNTRDRDAENLLSDERPERLWYSFGITNPG
                     SLTLNNYPNFLRNLSMPLVGDIDLAAIDVLRDRERGVPRYNEFRRQIGLNPITKFEDL
                     TKDAATLAELKRLYENDIEKIDTLVGQLAETVRPEGFAFGETAFQIFIMNASRRLMAD
                     RFYTKDYRPEVYTQEGIDWVEHTTMVDVLRRHNPQLQASLVGVENAFKPWGLNIPEDY
                     ESWPGANKQENLWVNGALRTQYAADQLPALQPVNVGGLIGSILWNKVKTRSDVAPPGY
                     EKPIHPHGVMAKVKFVAVPNNPYSGLFQGSDSGLLRLSVTGDPADRGFAPGLAWKAFV
                     DGKPSENVSALYTLSGQGANHNFFANELSQYVLPEANDTLGSTILFSAVSLKPTLLLL
                     NDMAEVTQAGAAVAKPKAPTQIYFVPRADLRNRFASTAHDFRTDLLTLNAGTKVYDVY
                     ATSMEIKSSIIPSISRSYAAQRRNSALKIGEMELTSAFIASAFGDNGVFFKHQRNEDK
                     "
     gene            complement(8620..10569)
                     /gene="gspD"
                     /locus_tag="VFF22_16075"
     CDS             complement(8620..10569)
                     /gene="gspD"
                     /locus_tag="VFF22_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HZX18370.1"
                     /translation="MTPTLSRLTFALLAAGLACSPLPLSAAVTAVAPSASQQEESWTI
                     NLKGADIREFIGQVAQISGQTFVVDPRVKGQVSVVSNSPLTLTEVYQLFLSVMATHGF
                     SVITQGDQARIVPNAEAKADADSGRNAPDRLETRLIQVQHGSATELIPLIRPLVPQYG
                     HLAAISSANAIIISDRTANIERIEDLLRQLDQKGQNDYSVLNLQHAWVMDTAEVLRSA
                     VDRGQAKGASGTQVIADGRTNRLILLGPPAARAKLVALAQSLDTPTSRSANTRVIRLR
                     HGDAKSLAETLGEISEKLKPEAGGETTGKPQTILIRADESLNALVLLAEPDVVATLED
                     IVRQLDVPRAQVMVEAAIVEVSGDITDALGVQWAVDARGSTGGLGGTNFSGTGLSIGR
                     VLQAIQDSGSDADNNTLGNLPDGAIIGLGTDSFGVLITALSANSKSNLLSTPSLLTLD
                     HQKAEILVGQNVPFQTGSFTTSGSGADNPFTTIERQDIGVTLKVTPHINDGASLRLEI
                     EQEISSIAPTSQGVNAVDLITNKRSIKSTILAENGQVIVLGGLIQDDVTQADSKVPLL
                     GDIPLLGRLFRSTKETRIKRNLMVFLRPTVIRDAAGMAALSGKKYSDIRVLGDIRENY
                     RPGILPRNPVQLFDQGEPEAIDLRE"
     gene            complement(10574..11218)
                     /locus_tag="VFF22_16080"
     CDS             complement(10574..11218)
                     /locus_tag="VFF22_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HZX18371.1"
                     /translation="MQLTATKRWLQRQATSLAGIVLILAMSISLAWQSADWLRLLRTP
                     VAASYSSTNEAPVIASNPEIGQLFGTSSAATDGPAPSTNLRLTLLGSFVHADPQRSTA
                     IILSEGSTAQRYSVNSEISSGVRLHSVAADRVELLRNGRRESLSFPLNSSTAGSLQNN
                     TQDAMADPLEQLNEMESDDLSQLRERMDALRQQMQDSGSLPETAEPTEQPTEDN"
     gene            11468..12955
                     /gene="gspE"
                     /locus_tag="VFF22_16085"
     CDS             11468..12955
                     /gene="gspE"
                     /locus_tag="VFF22_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HZX18372.1"
                     /translation="MNALLTEVPSRRLPFSFARRHGVVLVVADGLPCLAYRTGAELVA
                     LAEAQRFAGAQLPLHALSAEAFEQALAKAYQHDSASMQLAEDIGGSLDLAALAEQVPE
                     TEDLLEQEDDAPIIRLINAILGEAIKENASDIHLETFEKRLVVRFRVDGILREVLEPK
                     RELAALLVSRIKVMARLDIAEKRIPQDGRISLKVGGREVDVRVSTLPSANGERVVLRL
                     LDKQAGRLNLQHLGMSARDRDLMEATVRKPHGILLVTGPTGSGKTTTLYASLVSLNDR
                     TRNILTVEDPIEYHLEGIGQTQVNSKVDMTFARGLRAILRQDPDVVMVGEIRDKETAE
                     IAVQASLTGHLVLSTLHTNSAIGAITRLVDMGIEPFLLSSSLLGVLAQRLVRVLCPHC
                     KEAYQADEAECKLLGVPFAQPPTLYHARGCAECHQQGYRGRTGIYELVVFDDHLRTLI
                     HNAASEQDMTRHARTLGPSIREDGRRKVLEGATTVEEVLRVTREE"
     gene            12969..>13286
                     /locus_tag="VFF22_16090"
     CDS             12969..>13286
                     /locus_tag="VFF22_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspF"
                     /protein_id="HZX18373.1"
                     /translation="MAAFEYLALDGKGRQQKGVLEADSARQVRQILRERQLAPLDVKA
                     TRTRENAGAGSHFSFNRGISARDLALITRQLATLVQAALPIEESLRAAAAQASSPRIQ
                     SMLL"
BASE COUNT         2483 a         3908 c         4142 g         2753 t
ORIGIN      
        1 tgagtagggg ttgcgtcaca tgtgcggcat tgtcggtatc gtcggtaaat cgaacgtcaa
       61 tcaggcgctg tatgacgggc tcaccgtcct ccagcaccgc ggccaggatg ctgccggtat
      121 tgtcaccagc catgatggcc gcctgttcct gcgcaaggac aacggcctgg tgcgtgacgt
      181 gttccaacag cgccacatgc agcgcctggt tggccacatg ggcatcggcc acgtgcgcta
      241 cccgacggcg ggcagctcca gctcggccga agcgcagccg ttctacgtca actcgcccta
      301 cggcatcacc ctggcgcaca acggcaactt gaccaacgtc gagcagctgg ccaaggagat
      361 ctacgagtct gacctgcgcc acgtcaacac caattccgat tcggaagtgc tgctcaacgt
      421 gttcgctcac gagctggccc agcgcggcaa attgcagcct accgaagaag atgtgttcgc
      481 cgccgtcacc gacgtgcacg agcgctgcct cggtggctac gcggtagtcg cgatgatcac
      541 tggttacggc atcgtcggtt tccgcgaccc caacggcatc cgcccgatcg tcttcggtca
      601 gcgccatacc gatgaaggtg tggagtacat gatcgcctcg gaaagcgtct cgctggacgt
      661 gcttggcttc accctgattc gcgatctcgc gccgggcgaa gcggtgtaca tcactgagga
      721 aggcaagctg tttacccgtc agtgcgcgtc gaatccgaaa tacgccccgt gcatcttcga
      781 acatgtttat ctggcacgcc cggattcgat catggatggc atctcggtgt acaaggcgcg
      841 cctgcgcatg ggcgagaagc tggccgacaa gattctgcgt gagcgtccgc agcacgatat
      901 cgacgtggtt atcccgattc cagataccag ccgtaccgca gcactggaac tggccaacca
      961 tctgggcgtg aagttccgcg aaggcttcgt caagaaccgt tatatcggcc gcacctttat
     1021 catgccgggc caggcagcac gcaagaaatc cgtacggcag aagctcaatg ccatcgaact
     1081 ggaatttcgc ggcaagaacg tgatgctggt ggacgactcc atcgtgcgtg gcaccacctg
     1141 caagcagatc atccagatgg cccgcgaagc cggggcgaag aacgtgtact tctgctcggc
     1201 cgcgccggca gtgcgttacc cgaacgtgta tggcatcgac atgcccagcg cccatgagct
     1261 gatcgcgcat ggccgcacca ccgaggaagt ctgtgagctg atcggcgccg attggctgat
     1321 ctaccaggat ctgccggatc tgatcgaagc ggtcagtggt agcaagaagg tcaagatcga
     1381 caatttcgac tgtgcggtat tcgacggtaa atacgtgacc ggcgatatcg atgaggctta
     1441 cctggataag atcgagcagg cgcgcaacga tgcctcgaag gtcaagtccc aggccgtcag
     1501 cgcgattatc gacctgtaca acaactaggt tgcagccctg cccattacgg tgggccggtt
     1561 gaggggagtg gcaatgacga ttgaatggca agccgggcgt ctggacagcg atctcgatgg
     1621 cgtcggcttc gataccctgg cggtgcgcgc cggtcagcat cgctcgccag aaggtgagca
     1681 cagcgaggcg atctacccaa cctccagcta cgtattccgc accgcggccg atgctgccgc
     1741 gcgctttgcc ggcgaagtgc cgggcaatgt ctattcgcgc tacaccaacc cgacggtgcg
     1801 cgccttcgag gagcgcatcg ccgccctgga aggtgccgag caggcggtgg cgacctcgtc
     1861 cggtatgtcg gcgattctgg ccatcgtcat gagtctgtgc agcgccggtg accatgtgct
     1921 ggtgtcgcgc agcgtattcg gttcgaccat cagcttgttc gagaagtacc tcaagcgctt
     1981 tggcgtgcag gtcgactacg tgccgctggc tgatcttgac ggctgggctg ctgctttcaa
     2041 gcccaacacc aagctgctgt ttgtcgagtc gccgtccaac ccgttggccg aactggtgga
     2101 tattgctgcc ttggccgaga ttgctcatag caagggcgcg ttgctggcgg tggacaactg
     2161 cttctgcaca ccggcgttgc agcagccgct gaaactcggc gcagatgtgg tgatgcattc
     2221 ggcaaccaaa tacatcgacg gccagggtcg cagcatgggc ggcgtggtcg caggctcggc
     2281 caagctgatg accgaagttg tgggctttct gcgtaccgca gggccgacac ttagcccgtt
     2341 caacgcctgg attttcctca aaggcctgga aaccctgcgc attcgtatgc aggcgcattg
     2401 cgccagcgcg ctgcaattgg cgcagtggct ggagcagcag cccggcattg agcatgtgta
     2461 ctacgccggc ttacccagcc acccgcagca tgagttggcc aagcgtcagc agagtgcctt
     2521 cggtgcggtg gtcagctttg agatcaaggg cggcaaagag gcggcgtggc gttttatcga
     2581 tgccacgcgg gtgatctcga ttaccaccaa cctcggtgat accaagacca ccatcgccca
     2641 cccagcgacc acttcccatg gtcgtttgag cccggccgag cgtgctaacg ccggtattcg
     2701 cgacaacctg atccgcgtcg cggtgggcct ggaagatatt gctgatctgc aagccgattt
     2761 ggctcgcggc ctggcggcgc tctaagtcgt tctcctcacc tggcgggcct tcccggcccg
     2821 ctaggctgct tcatttctcc ccaaatgcat tcccttgcct cgccaaattc tcgggtgcgc
     2881 atggctgttg gcaaaccttt ggctaggcgc gggtaaaaca tgatgcgctt caaagtctga
     2941 aggtgcgcag gcgctagcat ccaccgccaa gcgcggcgtt tgaccgtaca aataataaga
     3001 acagggtctg cctgatggac cgacttgcgc cttgtgtatt acctgccaac ggtgtgctcc
     3061 gccagagtct gctggagctg ctggcgcgca gtgaagattt tgccctgacg ctgctgctgg
     3121 ccccggccgg ctccggcaag tccactttgc tgcaacactg gcgcaaccgc tgtgaggctg
     3181 cgctggtgca ttacccgctg caagcgcggg ataacgaccc gctgtgtttt tttcgccgcc
     3241 tggccgagag cattcgcgcc caggtgggcg atttcgacac gtcctggttc aaccctctgg
     3301 cactgacttc cagcctttca ccgcagctat tgggcgatct gctcggtgat gcgctagcgc
     3361 gggtcgaagg cccgctgttt atcgtgctcg atgattttca gtggatcgac tcatgcagca
     3421 ttcttgaggt gatggcggcg ctgatggaaa gggtgccagc ccatgttcat ctgatcatcg
     3481 ccagccgtaa tcaccctggt tttgccctga gtcagctgaa actggaaaac cgcctgctgt
     3541 gcatcgatca gcacgacctg cgtctgtcgg ccgaacaggt gcaacagctc aatagctgcc
     3601 tgggcggcca agcgctgagc caggcgtatg tcgacagcct gctgggtatg actgaaggtt
     3661 gggtggcggg ggtgaagatt gcgctgctgg cctatgcgcg ttttggcact caggcgctgc
     3721 agcgttttaa cggcagccag ccggaaatcg tcgattattt cggccatgtg gtgctgcgtc
     3781 agttgccgcc gaattttcgc gaatttctgc tatgtagcgg tctattcgag cgtttcgatg
     3841 gttcgctttg cgatcatgta ctgcagtgca cgggctcggc gttgttgttg gaggcgttgg
     3901 ccgagcgcca gctgtttttg ttgccggtgg agaaccagcc gggctggttc cgttttcatg
     3961 ccttgctaca ggactttctc tgccgccgcc tgcaagtcga ggagtatgcg cgcctggatc
     4021 agctgcacag ccgcgcagcc gactattacc tgagcttggg tgagcatgaa caggcgttac
     4081 agcatgcgca gcgttgtaag gatcaggcgc tgtttctcga catgctggag agtagttgcg
     4141 ccagctgggt tcgcagcggg cagttcggcg acatcctgcg ttggttggag cctttgtcag
     4201 aggcgttgct gctggcgcaa ccgaaactgc tcggctggtt gattgccgcg ctgtcgctgt
     4261 cgcggcgttt tcatcaggcg cattaccacc tggagttgct cgacagtttg gagcggagtg
     4321 cgcctggcgc caatctggaa ccggcgacgc ggcagtttct cgcgctattg ctgggcgtgt
     4381 tcgagcagga caaggacttt atgccgccat ccgcctggcg tgacttgttg ggaacggtgc
     4441 agaacctgga agtccgcgcg tgcaccctgg cggtgattgc ctatcagcat ctgttggccg
     4501 cgcgcctgca ggatgctatc cgcttcgcca gcgagtccaa gcaattgctc gcacagtgtg
     4561 ggtacagctt tctggaaagt tacaccgatc tgatcatcgc gctttgtcac cgcaatgccg
     4621 ggcgcgccac tcacgcgcgc aagcaggtgt gcctggatta tcaggccact gatccagcct
     4681 ctccggcctg ggtaaaccgc gccacggcca tggtggtggt gctgtatgag caaaatcaac
     4741 tggtcgaggc gcagcagcta tgtgacgagc tgatggcgat ggtcaattcg tcctcggcca
     4801 ccgaggcgat tgccacggtc tacatcactt tgtcgcggct gcagcatcgc cagcagcaga
     4861 cggcgcgcgc cagtcgtttg ctcgaccagt tgtggtgcat tctgcaactg ggtaactacg
     4921 cacgttttgt cagccaggtt gcccaggaag gcatgcgcca ggcatacctg agcggtaaac
     4981 atgctgccat ggacgccgtg gctcagcgtt ttgacttgcc ggcgcggttg caggccggtg
     5041 agtgggagag cgtgcgcacc tatgatgaaa gctgggagcg cttgggcctg gcctgtgtgt
     5101 actggctgca ggcacgtggc gggcaggctc aggccgagcg gattctcaag gtgctggcag
     5161 cttcattgcg gcaaagcgag atgcgcgcgc gactgttgat cgtcgaagcc aaccgtctgg
     5221 tactggcggc gtccagccag agcaagagcc agcgactggc ggcgctgggc ggcttggtcg
     5281 agacctatgg catcgtcaat atcaaccgct cggtatttga tgaagcccca ggtttcggcg
     5341 cgggcgtgct gagcctggcg caggcagaat tgctcaacgt tccggagaag taccgtgaac
     5401 actacggtga gtttctccag gcgccgcagc tgctgccaca aaccagtgcg ctgtcgacca
     5461 gcctgttgac cgggcgtgag gtggaggtgt tcgagtgcct gctcagcggc ctgtcgaaca
     5521 cccagatcag cgacaagacc ggtattgcct tgtccaccac caagtggcac ctgaaaaaca
     5581 tctattccaa gctcaatgtc tccagccgca ccgaggcgat tctcgccgta cggccgcgcg
     5641 tctcgctcaa ctagaccgcc ctgcataact gattggccat agcccctccg ctggaagggg
     5701 gcgttgcgcc atacccgtcg ataggcttga tcctgcgctg ctatgtcagt agccgccttg
     5761 tccgtacaac aataaaaacg ggagcaatag ccatgtcggt atgggtcact tggccagctt
     5821 tgaccaagct gggaacgttg ggcgtagtct ccggactact ggtgttggcc gtcgagcgcc
     5881 aggcgttgtt cgagaacaac ctctttgatg ttgaaaacta cgcgggctac aacgccgata
     5941 tcacctgcga tgcgcgcagt ttggcggccc gtaccgagga cggcacctgc aacattctca
     6001 gcaacccggc cgagggctcg gtgtaccggc gcttcggccg taacgtcaat ccggcggtga
     6061 ctcaaggtga aaccaccacc ttgctcagcc ccaacccacg tgaggtgagt aacagcctga
     6121 tggcgcgcga ggagttcaaa ccggcaccca gcctgaactt tatcgccgcc gcctggattc
     6181 aatttatggt gcacgactgg tttgatcacg gtccgaatgc cgacgctgac ccgatccagt
     6241 tcccgctacc acccggtgat gtgctgggta gcggcagtat gtcggttaag cgcactcagc
     6301 cggacccgac gcgcagcagc gccgacgccg gcaagccgca aacctaccgc aaccacaaca
     6361 cccattggtg ggacggctcg cagctgtatg gcagcagcaa ggaaaccaac gataaggtgc
     6421 gttcctttgt tgacggcaag cttaaggtca acgccaatgg caccttgcct accgagctgg
     6481 tcagcggcaa accagtcacc gggttcagcg agaactggtg gcttggcctg agcatgctgc
     6541 atcagctgtt taccctcgaa cacaacgcta tcgccagcag gctcaaacag aagtatccgg
     6601 cgcagagcga tcagtggctg tacgaccgcg cgcggctgat caactcggca ctgatggcca
     6661 agatccacac cgtggaatgg actccggcgg tgatcgccaa cccgatcacc gaacgggcca
     6721 tgtacgccaa ctggtggggg ctactgggtc agggcgggcc gcgtgacgat taccaggcgg
     6781 aagtgcgcat gttgcaggag gatctggcca agtccgattc tttcgtcaag cgtatcctcg
     6841 gttttgatcc caatgtcgct ccaggggttg gcaacagtgc cttcgaccac gccttcagcg
     6901 gcatcgtcgg ctcgactgcg ccgaacaact acggcgtgcc gttcaccctc actgaagagt
     6961 ttgtcgcggt gtaccgtatg cacccgctga tgcgcgacaa cgtgcaggtg tatgacatcg
     7021 gctccaacca ggtttcacgc accatcgcgc tgcagaacac ccgtgaccgc gacgccgaaa
     7081 acctgctgag cgacgagcgc ccggagcgtc tgtggtactc cttcggcatc accaacccgg
     7141 gttcgctgac gctgaacaac tacccgaatt ttctgcgtaa cctgtcgatg ccattggtgg
     7201 gcgatatcga cctggcggcc atcgacgtgc tgcgtgaccg tgagcgtggc gtgccgcgtt
     7261 acaacgagtt ccgccggcag atcggtctca acccgatcac caagttcgag gacctgacca
     7321 aggacgccgc aaccctggcc gaactcaagc gcctgtatga aaacgacatc gagaagatcg
     7381 acacactggt aggccaattg gcggaaactg tccgcccaga aggctttgcc ttcggtgaaa
     7441 cggcgttcca gatttttatc atgaacgcct cgcgccgcct gatggccgac cgcttctaca
     7501 ccaaagacta ccgccctgag gtgtacaccc aggaaggtat cgactgggtt gagcacacca
     7561 ccatggtcga tgtgctgcgt cggcataacc cacagctgca agccagcctg gtcggtgtgg
     7621 aaaacgcctt caaaccctgg gggctgaata ttccggagga ttacgaaagc tggccagggg
     7681 ccaacaagca ggagaatctc tgggtcaacg gcgcgctacg cacccagtac gcggcagacc
     7741 agctgccggc gttgcagccg gtgaatgtcg gtgggctgat cggctcgata ctgtggaaca
     7801 aggtgaaaac ccgcagtgac gtggcaccgc ctggttacga gaagccgatc catccccatg
     7861 gcgtgatggc caaagtcaag tttgttgcgg tgccgaacaa cccctatagc ggcctgttcc
     7921 agggttccga tagcggcctg ctgcgtttgt cggtgacggg tgacccggct gaccgtggct
     7981 ttgcaccggg tctggcgtgg aaggcctttg tcgacggcaa accgtcggag aacgtttcgg
     8041 cgctgtacac cctgagcggg cagggggcca accataactt cttcgctaac gagctgtcgc
     8101 agtatgtgct gccggaagcc aacgacaccc tgggttctac cattctattc tctgcggtca
     8161 gcctgaaacc gacgttgctg ctgctcaatg atatggcgga agtcacccag gcaggcgctg
     8221 ccgtggccaa acccaaggcc ccgacgcaga tctactttgt gccgcgcgct gatctgcgca
     8281 accgcttcgc tagcacggcc catgattttc gcacggatct gctgaccctg aatgccggca
     8341 ccaaggtcta tgatgtttac gctacgtcga tggagatcaa gagctcgatc attccgtcca
     8401 tcagccgcag ctacgccgct cagcggcgca acagtgcgtt gaagattggc gagatggaat
     8461 tgacctcggc ctttatcgcc tcggctttcg gtgacaacgg ggtgttcttc aagcatcagc
     8521 gtaacgaaga caaataagcg ttagtcacgc taaaccaaaa ggcctcggta gataccgagg
     8581 cctttttatt tctatgccgt tctatacgtg gcgcggtgat tactcgcgca gatcgatagc
     8641 ctcaggttcg ccctggtcga acagctgcac cgggttgcgt ggcaggatgc cggggcggta
     8701 gttctcgcgg atatcgccaa gcacgcggat gtcgctgtat ttcttcccgg ataacgccgc
     8761 catgccggcc gcatcgcgga ttacggttgg gcgcaggaac accatcaggt tgcgcttgat
     8821 gcgggtttcc ttggtcgagc ggaacagtcg gccgagcagc gggatatcac cgagcaacgg
     8881 taccttggag tctgcctggg tgacgtcatc ctgaatcagc ccgccgagca cgatgacctg
     8941 gccgttttcg gcgaggatgg tgctcttgat tgagcgcttg ttggtgatca ggtcgacggc
     9001 attcacgccc tggctggtgg gggcgatgga ggagatttcc tgctcgattt ccaggcgcag
     9061 gctggcacca tcgttgatat gcggagtgac tttcagggtc acgccgatgt cctggcgctc
     9121 aatggtggta aaggggttgt cggcgcccga accgctggtg gtaaaggagc cggtttggaa
     9181 gggtacgttc tgcccgacca ggatttccgc cttctggtgg tcaagggtca gtaggctcgg
     9241 tgtcgacagc agattgctct tactgttggc cgatagtgcg gtaatcagca caccaaaact
     9301 gtcagtgccc aggccgataa tcgcgccgtc cggcaggttg cccagggtat tgttatcggc
     9361 gtcactgccg ctgtcctgaa tggcttgcaa tacccggccg attgacagcc cggtgccgct
     9421 gaagttggta ccgcccaggc cgccagtgct gccgcgggcg tccacggccc attgcacgcc
     9481 cagagcgtcg gtgatatcgc cggacacttc aacaatcgcc gcctcgacca tgacctgggc
     9541 gcgcggtaca tccaactggc gcacgatatc ttccagggtt gctaccacat cgggctcggc
     9601 cagcagcacc agggcgttga ggctttcgtc ggcgcggatc aggatggtct gcggtttgcc
     9661 ggtggtttcg ccgccggctt ctggtttgag tttttcggaa atttcgccga gggtttccgc
     9721 caggctcttg gcatcgccat ggcgcaggcg gatgacccgg gtattggctg agcggctggt
     9781 gggggtatcc agcgattgcg ccagggccac cagtttggcc cgtgctgccg gtgggccgag
     9841 caggatcagg cggttggtgc ggccgtcggc aatcacctgg gtgccggagg cgcccttggc
     9901 ctggccgcga tctacggcgc tgcgcagcac ttcggcagtg tccatgaccc aggcatgctg
     9961 caggttgagc acgctgtagt cgttctggcc tttctggtcg agctggcgca gcaggtcttc
    10021 gatgcgttcg atattcgccg tgcggtcgct gatgatgatc gcattggcgc tgctgatcgc
    10081 cgccaggtgg ccgtattgcg gcaccagtgg gcggatcagc ggaatcagct cggtggctga
    10141 gccgtgttgt acctggatca gccgggtttc caggcggtcc ggtgcgttgc gtccgctatc
    10201 ggcatcagct ttggcctcgg cgttaggcac gatgcgcgcc tgatcgccct gggtaatcac
    10261 gctaaagccg tgggtggcca tcaccgagag aaacagctga taaacctcgg tcagcgtcag
    10321 cgggctgttg gacaccacgc tgacctggcc cttgacccga ggatcgacaa caaaggtctg
    10381 cccactgatc tgcgcgacct ggccgataaa ctcacggata tcggcgccct tgaggttgat
    10441 ggtccagctt tcttcctgct ggctggcact cggcgctacg gccgtaacgg cagcactcag
    10501 tggcagcggg ctacaggcca ggccagcggc cagcagggcg aaggtaaggc gcgagagagt
    10561 cggagtcatc gtgtcagttg tcttccgtgg gctgttcggt gggttctgca gtttcaggca
    10621 ggctgccgga gtcctgcatc tgctggcgca gggcgtccat ccgctcgcgt agttggctca
    10681 gatcatcgga ttccatctca ttaagctgct caagcggatc ggccatggcg tcctgggtgt
    10741 tgttttgcag gctgccagcg gtcgagctgt tgagcgggaa actgaggctt tcacggcggc
    10801 cattgcgcag cagttcgacg cggtcggctg ccactgaatg caggcgcacg ccgctgctga
    10861 tttcgctgtt gacgctgtag cgctgggccg tgctgccttc gctgaggatg attgcagtgg
    10921 agcgttgtgg atcggcatgg acaaagctgc cgagcaaggt caggcgcagg ttggtgctgg
    10981 gggcggggcc atcagtggcg gcgctgctgg tgccgaacag ttggccgatt tcagggttac
    11041 ttgcaatcac cggcgcttcg tttgtgctgc tatagcttgc agctactggc gtgcgcagca
    11101 aacgcagcca atcggcgctc tgccaggcca ggctgatgct catggcaaga atcagcacga
    11161 tgccagcaag ggaggtggcc tggcgctgta gccagcgttt ggtagccgta agctgcaagg
    11221 ggggcgtcac tccgtaactt attattatgt ggtgtggttc gcgcgctgat catagacatt
    11281 tgccaagcat ttggcagccc ctggttgggg tagtgtgtag accagttcaa tgtgctaaag
    11341 atagcgcgct gtttttcgct ctattgcagc taacttacaa caactatgtt gcaaaacccc
    11401 agtttttgag ccttcgcttg atgtcctggg gccagtatca acttcctgtg cttaaggcat
    11461 cttacggatg aatgcgttgc ttacagaagt gccatcacgg cggttaccct tcagctttgc
    11521 caggcgtcac ggtgtggttc tcgtcgttgc ggacggccta ccttgcctgg cttatcgcac
    11581 gggtgccgag ctggtggcgc tggccgaggc gcagcgcttt gctggtgcgc agttaccgct
    11641 gcatgcgctg tcggcagagg ccttcgagca ggccctggcc aaggcctatc agcatgattc
    11701 ggcgtctatg cagttggccg aggatatcgg cggtagcctg gatctagcag cgctggccga
    11761 gcaggtgccg gaaaccgagg acctgctgga gcaggaagac gacgcaccga tcattcgcct
    11821 gatcaacgct atcctcggcg aagcgatcaa ggaaaacgcc tcggatattc acctggagac
    11881 cttcgagaaa cgccttgtcg tacgcttccg cgtggacggc attctgcgtg aggtgctgga
    11941 gcccaagcgc gagctggcgg ccttgctggt gtcgcggatc aaggtgatgg cgcggctgga
    12001 tatcgccgag aagcgcattc cccaggatgg tcggatttcc ctgaaagttg gtggccgtga
    12061 agtggatgtg cgggtctcga cccttccatc ggccaacggc gagcgggtgg tgctgcgttt
    12121 gctcgataag caggccggtc gtctgaattt gcagcacctg ggcatgagcg cgcgcgaccg
    12181 tgaccttatg gaagccaccg tgcgcaaacc ccacggcatt ctgctggtca ccggccccac
    12241 cggctcaggt aagaccacca ccctttatgc cagcctggtc agcctcaatg accgcacgcg
    12301 caatatcctt accgtcgaag acccaatcga atatcacctc gaaggcatcg gccagaccca
    12361 ggtcaattcc aaggttgaca tgacctttgc ccgtggcctg cgcgccatcc tgcgccagga
    12421 ccctgacgtt gtgatggtcg gtgagattcg cgacaaggaa accgccgaaa tcgccgtgca
    12481 ggcctcgttg accggtcacc tggtgctctc gaccctgcac accaacagtg ccatcggcgc
    12541 gattacccgt ctggtggaca tgggtattga gccgttcctt ctgtcgtcat ccttgcttgg
    12601 cgtgctggct cagcgcctgg ttcgggtgct ctgtccgcat tgcaaagagg cctaccaggc
    12661 cgacgaagcc gagtgcaaat tgctcggcgt gccgttcgca cagccaccga ccctgtacca
    12721 cgcccgcggc tgcgccgagt gccatcagca gggctaccgc ggccgtaccg gtatctatga
    12781 gctggtggtg tttgatgacc acctgcgcac cctgattcac aacgccgcct ccgaacagga
    12841 catgacccgc cacgcccgca ccctcggccc gagcatccgt gaggatggcc ggcgcaaggt
    12901 gctggaaggg gcgaccacgg ttgaggaagt gctgcgcgtg actcgcgaag aatgagcggg
    12961 gaagaataat ggccgcgttc gaatacctcg ccctcgatgg caaaggccgc cagcagaaag
    13021 gcgtgctgga agctgacagt gcgcgccagg tgcggcagat tctgcgtgag cggcaactgg
    13081 cgccgctgga tgtcaaagcc actcgcaccc gtgaaaatgc tggcgcaggc tcacatttca
    13141 gttttaaccg aggcatctcg gcgcgggatc tggcgttaat cacccggcag ttagcgactt
    13201 tggtgcaggc tgccttgcct attgaggagt ctttgcgcgc tgcggccgcc caggccagtt
    13261 cgccacgtat ccaaagcatg ctgctg
//
LOCUS       DASRUM010000243         8666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_463058, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000243 DASRUM010000000
VERSION     DASRUM010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8666
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463058"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VFF22_16095"
     CDS             <1..973
                     /locus_tag="VFF22_16095"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HZX18374.1"
                     /translation="GSALQRPSLDVVDAAQSYLLGIAQAGERLVAVGERGIVVLSDDS
                     GATWRQVRTPVSVTLTMVRFVDAQHGYAVGHGGSVLVSRDGGESWSLSLDGHRAAQIL
                     LAATQSRGNAREIAEAKRLIDDGPDKPFLDVLVRDPLNAFVVGAYGLALSTADGGKSW
                     QPWLPRSVNPGGMHLNAIRGLGERLVIAGEQGLVLLSDDGGASFRSLETPYSGSFFTV
                     ELPSEHAIVVAGLRGNILRSENDGDSWAPLISPMEASITASAIRPNGALVFVNQAGML
                     MHERAGHLLPINRHPLPPLNDVLPTSQAGTVLVSGQGIRVLNAGDLK"
     gene            970..3411
                     /locus_tag="VFF22_16100"
     CDS             970..3411
                     /locus_tag="VFF22_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014338059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZX18375.1"
                     /translation="MKSSQTAPVGNTADFDPRSGSLLERILFNNRLLVILSCAIVTLL
                     LAWQTTKLELNASFEKTIPSQHPYIQVFHQYQSQLTGLGNSVRIAVQSPSGDIYDPAY
                     LDILRRLSDEVFLLPGVDRVNMKSLWTPSTRWTGVTEDGLDGGSVIPNGYDGSPASLE
                     QLRANVARSGEIGQLVAANGRSTVIYVPLLNKDAQGQSLDYATFANHLEELRQKYEAE
                     GLTVNVTGFAKIIGDLIDGVRAVLVFFAIAVLIATITVFWYTRCARSTLLVVTASLVA
                     VVWQLGLLPTLGYSLDPYSILVPFLVFAIGMSHGAQKMNGIMQDIGRGIPKLLAARFT
                     FRRLFLAGMTALLADAVGFAVLLVIDIQVIRELAIAASIGVAVLIFTNLILLPVLLSY
                     TGVSASAAKRSLRAEQADASGQDKHALWSFLDRFTRRPMAGIAVVGALVIGVGGFIVS
                     TQLKIGDLDPGAPELRADSRYNRDVAFMNANYGASSDVLAVMVTSPDGQCAQYDTINK
                     LDALEWQLRQLPGVESTNSLALLNRRMLVGLSEANPKWYEFMPNQGMLNTVAASAPRG
                     LYNDGCSLLTLHVYLRDHKADTLTGVVNHVEAFARANDTEGVKFLLAAGNAGIEAATN
                     IVVKEAWWQMLFLVYAAVALLSLITFRSWRAVLVAILPLMLTSILAEALMVVLGMGVK
                     VATLPVIALGVGIGIDYALYILSVTLAQLREGKSLSEAYYRALLFTGKVVMLTGVTLA
                     IGVITWVLSPIKFQADMGLLLAFMFLWNMLGALILLPALAHFLLKPAQLAVKAHSTRT
                     QPAPDKSPRTHVTPA"
     gene            3505..5163
                     /locus_tag="VFF22_16105"
     CDS             3505..5163
                     /locus_tag="VFF22_16105"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014755991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZX18376.1"
                     /translation="MTETGQAVVLRNGAIYTLNVQQPWAEALAIHDGRIVAVGSEQQV
                     LAAVSADATIIDLQDKMLMPGMADIHNHFLIAGRAELYEVNFLPNTPLDVIIEKVRER
                     AKEAPLDRWITGGIWGSNMLSELTYEARLRLDEAAGGRPVMLNDDSHHNRWLNAAGLK
                     LCGIDNDTPNPPNGTIVRRPETGEATGLLLEAASFLTEGPIGAALTAEPELNVAAGVH
                     ALKRLNACGITAVQEPLTGRFIMDALKTMAERNLLTTWVVGSMPVLQGPLASSEWGHD
                     LFALRDAYRSQYFRPEFGKLFMDGVPITYTSAMLEPYAPNEAHGCCFRGSNFMTVPQL
                     ARVIADCEKADILLKIHCTGDGSVRAALDAFEVVRSFNGGSRRHQIAHAGYVHPDDVP
                     RFVELGIVADLSPMLWFPGIIVEQLKLVLSEERVNNSHRHVDMDKAGVLMAAGSDWPV
                     LPDPNPFIGLQGIITRRDPTGHFPGVMGAEQGLDLATALRIYTLDPVRAMGLDAETGS
                     LEVGKSADFIVLDRNLFQTPVDQLVETRVLKTYFAGKLVYEVDA"
     gene            5160..6221
                     /locus_tag="VFF22_16110"
     CDS             5160..6221
                     /locus_tag="VFF22_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014755990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZX18377.1"
                     /translation="MSALEEARTPVILLTGFLGSGKTTVLNQLLQLPEMADTAVIINE
                     FGEVGLDHLLVEQVSENLRLLQSGCLCCTVRGDLVDTLIDLYERRQHHTFPAFSRVII
                     ETTGLADPTPVLQTFMIDPGVTSHYRLAQVVTLVDAVNGMRTLDMHTEALKQVTLADR
                     LLLSKTDLTDPVTITKLEQRLADLNPGAEVRAILHGQVSEQDILSVVERQPIDLDAYL
                     AQTENGYIQSSHRDGAVQSHCLVLDQPVEEARLAHWLELMATLRGENLLRIKGLVKVV
                     EHPDQPTVVHGVQQIVHPLQQLPAWPSNDTRSRIVLIVMDIDYAEISRTFERFVGVRL
                     PAFRPLNSLKQISPLQAPI"
     gene            6467..7708
                     /gene="tyrS"
                     /locus_tag="VFF22_16115"
     CDS             6467..7708
                     /gene="tyrS"
                     /locus_tag="VFF22_16115"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZX18378.1"
                     /translation="MNTPQDPIQFLQERGFIHQSTDLEGLREHLQQGCATIYLGFDAT
                     ADSLHVGHLQGLMVMRWQQRFGHRPILLIGGATTRIGDPSFRDESRPMLSEAQIQHNI
                     VGICETFARFVNLGPSGNDGLVVDNADWLDGMGYLQFLNQVGRHFSINRLLTFDAVRQ
                     RLDREHSLSFQEFGYTLLQAHDFTELAKRHGCSVQLGGADQWANIINGVELARRQDGR
                     QLFGLTMPLLTTSDGRKMGKSAQGAIWLNPQRLSPFDFWQFWRNCDDRDVCRFLALFT
                     ELPLAEIANLAQAEGAQLNAAKVRLASAVTALVHGEEAAQLAEATAQGLFAQGGELQG
                     LTTLSVPRSQLAAGYSLIELLEQHELQPSRGATRRLVAGGGLRLDGETVVDADLALPS
                     DGDGWLLSLGKKHHYRIQLEG"
     gene            7852..>8666
                     /locus_tag="VFF22_16120"
     CDS             7852..>8666
                     /locus_tag="VFF22_16120"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HZX18379.1"
                     /translation="MQNAYWAAMPIWVVAQPSRGLLLERALYRLLGTLLGALAGFVVL
                     TQASSLLQLMLLAAVIALCAGMTHLLRGTSAYAWLMAGMTAAVVVIPSALSPQNSWTL
                     ALARVECTLIGVLAVTLVLWLFTPKTRRADFYHRARALAADAVDFAAQSLSGGTDPAT
                     ELRILKALSDIDGAARPISAGSLRGYRHLRHLDQLLLASLNIMAAGQAMGCHLRPEAQ
                     AAAALASRLQQVAERLRSEEPADSILSAPPANLPPRRLARLEQALEQLCQAAA"
BASE COUNT         1621 a         2792 c         2584 g         1669 t
ORIGIN      
        1 cggttcggcc ctgcagcggc cctcgttgga cgtggtcgat gctgcgcaga gctatctgct
       61 gggcatcgcc caggccggtg agcgcctggt ggctgtgggt gagcgcggca tcgtggtgct
      121 ctccgacgat tccggcgcca cctggcgcca ggtgcgcacg ccggtcagcg tcaccctgac
      181 tatggtgcgt ttcgtcgatg cgcagcatgg ttatgccgtc ggtcatggcg gcagcgtgct
      241 ggtcagtcgt gatggcggcg agagctggtc cttgagtctc gacggacacc gcgccgcaca
      301 gatcctgctc gcggcgacgc agagccgagg caatgctcgg gagatcgctg aggccaagcg
      361 cctgattgac gacggaccgg acaaaccctt ccttgatgta ctggtgcgcg acccgctcaa
      421 tgccttcgtg gtgggtgcat acgggctggc cctgagcacc gcagacggcg gcaagagttg
      481 gcaaccctgg ctgccgcgca gcgttaaccc gggcggcatg cacctcaatg ccattcgcgg
      541 tctcggcgag cgtctggtca tcgcagggga acagggtctg gtgctgctct ccgatgacgg
      601 tggggccagc ttccgcagcc tcgaaacacc ctactccggg agtttcttca ccgtcgagtt
      661 gccttccgag cacgcgatcg tggtcgccgg tttgcgcggc aacattctgc gcagcgaaaa
      721 cgacggcgat agctgggcac ctttgatctc gccgatggag gcgtccatca ctgcctcggc
      781 aatccggccc aacggcgcgc tggttttcgt caaccaggcc ggcatgctga tgcatgaacg
      841 ggccgggcat ctgctgccga tcaaccgcca ccccttgccg ccgctgaacg atgtgctacc
      901 cacctcacag gcgggcaccg tgctggtgag tggccaggga atccgtgtgc tgaatgctgg
      961 agatctgaag tgaagagttc gcagacagcc cccgtgggaa acactgccga tttcgatccg
     1021 cgttccggtt cgctgctgga acgcatactg ttcaacaacc gcctgctggt gatcctgtcg
     1081 tgcgccattg tcactctgct tctggcgtgg caaacgacca agctcgagct gaacgccagc
     1141 tttgagaaaa ccattcccag ccagcatccc tacatccagg tattccacca ataccagagt
     1201 caattgaccg gtctgggcaa ctcggtacgg atcgccgtac agagcccgtc gggcgatatc
     1261 tacgacccgg cctacctcga catcctgcgc cgcctctccg atgaggtgtt tctgctgcca
     1321 ggggttgatc gggtcaatat gaaatcgctg tggacgcctt cgacacgctg gaccggggtg
     1381 accgaagacg gcctggacgg tggctcggtg atccccaacg gttatgacgg cagcccagct
     1441 agcctcgagc agttgcgcgc caacgtcgcc cgctccgggg agatcggcca actggttgca
     1501 gccaatggcc ggtccacggt gatctatgtg ccgctgctga acaaggacgc ccaaggccag
     1561 tcgctggact atgcgacctt cgccaaccac ctggaagagc tacggcaaaa gtatgaggcc
     1621 gagggtttga ccgtgaacgt caccggcttc gccaagataa tcggtgacct gatcgatggc
     1681 gtgcgtgccg tgctggtgtt cttcgccatc gcggtgctga tcgccacgat tacggtgttc
     1741 tggtacaccc gctgcgcacg ctccaccctg ctggtggtga ctgcgtcgct ggtagcggtg
     1801 gtctggcagc tcgggctgct gccaaccctg ggctattcgc ttgatcctta ttcgatcctg
     1861 gtgccctttc tggtcttcgc cattggcatg agccacggcg cacagaagat gaacggcatc
     1921 atgcaggaca tcggtcgtgg cataccgaaa ctgctggcgg cgcgctttac cttccgccgt
     1981 ctgttcctgg ccggcatgac cgccctgctg gctgatgcgg tcggcttcgc ggtgctgctg
     2041 gtgattgata ttcaggtcat ccgcgagctg gcgatcgcgg cgtctatcgg cgtcgcggtg
     2101 ctgattttca ccaacctgat cctgttgccg gtgctgctct cctacacggg ggtcagcgcc
     2161 agtgctgcca aacgcagcct gcgggccgag caagccgacg ccagcggtca ggacaaacat
     2221 gcactgtggt cgttcctcga ccgctttacc cgtcggccga tggccggtat cgcggtggta
     2281 ggcgcgctgg tgataggcgt cggcgggttt atcgtcagca cgcaactgaa gatcggcgac
     2341 ctcgaccccg gtgcgccgga gctgcgcgcc gacagtcgct acaaccgcga cgtagccttt
     2401 atgaacgcca actatggcgc ctccagcgac gtactcgcgg tgatggtgac cagtccagat
     2461 ggccagtgcg cgcagtacga caccatcaac aaactcgatg cgctggaatg gcagctgcgc
     2521 cagttgcccg gggtcgagtc gaccaactcg ctggcactgc tcaaccgccg catgctggtc
     2581 ggtttgagcg aggcgaatcc gaaatggtac gagttcatgc ccaaccaggg catgctcaac
     2641 acggtcgccg ccagcgctcc acgtggcctg tacaacgatg gctgtagcct gctcacgctg
     2701 cacgtctacc tgcgcgacca caaggccgac accttgaccg gcgtggtcaa ccatgtcgag
     2761 gcatttgccc gcgccaacga cacagagggc gtcaagttcc tgctggcagc cggcaatgcc
     2821 gggatcgaag cggcaaccaa catcgtggtc aaggaggcct ggtggcagat gctattcctg
     2881 gtctatgccg cagtggcgct gctgagcctg attaccttcc gctcctggcg cgcggtgcta
     2941 gtggcaatcc tgccgctgat gctgacctcg atactggccg aagcgctgat ggtggtactt
     3001 ggtatgggcg tcaaggtagc gactttaccg gtgatcgccc tgggggttgg aatcggtatc
     3061 gactacgcgc tgtacatcct ctcggtaacc ctggcccagt tacgcgaagg caagagtctg
     3121 tctgaggctt attaccgtgc cctactgttt accggcaagg tcgtgatgct gaccggcgtg
     3181 accctggcca tcggcgtcat cacctgggtg ctcagcccga tcaaattcca ggccgacatg
     3241 ggtctgctgc tggccttcat gttcctgtgg aacatgctcg gcgcgctgat cctgcttccg
     3301 gcgcttgcgc actttctgct caagcctgcg cagctggcgg ttaaggctca ttccacgcgc
     3361 acgcagcctg ccccagataa atcaccgcgt actcatgtaa cgccggcttg accactactc
     3421 accactgcca ctgatctagc gtcgatgtgc ctgagcatca ggcccatcgg cacttaccga
     3481 aacagctcga aaggacttac ccacatgacc gagacaggac aggctgtcgt tctgcggaac
     3541 ggcgcgatct acacactcaa tgttcagcaa ccatgggccg aggcattggc cattcacgat
     3601 ggccgcatcg tggccgtcgg cagcgaacag caggtgctcg cagccgtaag cgcagatgcc
     3661 acgatcattg acctgcaaga caagatgctg atgccgggga tggcggatat tcacaaccat
     3721 ttcctgattg ctgggcgcgc agaactctac gaggtcaact tcctgcctaa caccccactc
     3781 gatgtgatca tcgaaaaagt gcgcgaaaga gcgaaagagg cgccgctcga tcgctggatc
     3841 accggcggta tctggggcag taacatgctc agtgaactca cctatgaagc gcgcctgcgc
     3901 ctggatgagg cggccggtgg aaggccggtg atgctcaacg acgactcaca ccacaatcgc
     3961 tggctgaacg cagctgggct caagctgtgc ggcatcgata acgacacacc gaatccaccc
     4021 aacggcacta tcgtgcgccg ccctgaaaca ggtgaggcga cgggtctttt gctcgaagcg
     4081 gcgagctttc ttaccgaggg gccaatcggc gctgccttga ctgccgaacc agaactcaat
     4141 gtcgcggcgg gcgtgcatgc gctcaaacgc cttaatgcct gcggcatcac cgccgtgcaa
     4201 gagccgctga ccgggcgttt tatcatggat gcactcaaaa ccatggcaga gcgtaatctg
     4261 ctgaccacct gggtagtcgg ctcaatgccg gtactacaag gaccactcgc cagcagtgaa
     4321 tgggggcacg acctattcgc cctgcgcgac gcctaccgca gccagtactt ccgtcctgaa
     4381 ttcggcaagc tgttcatgga tggcgtacct attacctaca catcggcgat gctggagcct
     4441 tacgcaccga acgaagccca tggatgctgc ttccgtggtt ccaacttcat gaccgtgcct
     4501 cagcttgcca gggtcatcgc cgactgtgaa aaagccgaca tccttttaaa gattcactgc
     4561 acaggcgacg gctcggttcg cgcggccctc gacgccttcg aggtggtgcg cagcttcaac
     4621 ggtggcagcc gtcgtcatca gattgcccac gcaggctacg tgcatccgga tgacgtgccg
     4681 cgctttgtcg agctgggcat cgttgccgat ctatcgccga tgctttggtt tcccggcatt
     4741 atcgttgagc aactcaagtt agtgctgtcc gaagagcggg ttaacaattc acatcgccac
     4801 gtcgacatgg acaaagcggg tgtgctgatg gctgcgggct cagactggcc ggtactgcct
     4861 gaccctaatc cgtttattgg actgcagggc attattactc gccgcgaccc tactgggcat
     4921 ttcccaggcg tgatgggagc cgaacagggt ctggacctgg ctaccgcact gcgtatttac
     4981 acgttggacc ctgtcagagc catgggcctg gatgcggaga ccggctccct agaggtcggt
     5041 aaatccgctg atttcatcgt gcttgatcgc aacctctttc aaaccccggt cgaccagttg
     5101 gtcgaaacac gcgtgctcaa aacctacttt gccggcaagc tggtgtacga ggttgacgca
     5161 tgagtgcgct agaggaggcc cgcacgccag tgatattact cactgggttc ctgggcagcg
     5221 gcaagacaac ggtactgaac caactgctcc aactgccaga gatggctgac acagcggtaa
     5281 ttatcaacga gttcggtgag gtcgggctcg atcatctgtt ggtcgagcag gtcagcgaaa
     5341 acctacgact gctacaaagc ggatgcctgt gctgcacagt caggggcgac cttgtcgata
     5401 ccttgataga cctgtatgag cgtcgccagc accacacatt tccggccttc tcccgggtga
     5461 ttatcgagac caccgggcta gccgacccaa cgcctgtgct gcaaacgttc atgatcgacc
     5521 ccggggtcac ctctcactac cggctcgcgc aagtggtcac cttggtggat gcagtcaacg
     5581 gcatgcgcac gctggatatg cataccgagg cactcaaaca ggtgacgctt gccgatcggt
     5641 tgctgctgag caaaaccgac ctcactgacc ctgtcacgat caccaaactt gagcagcgcc
     5701 tggcagacct caaccccggc gccgaggtgc gcgccatact tcatggccaa gtatcggaac
     5761 aggacatact cagcgttgta gagcgccagc ctatagactt agacgcttac ctggcccaga
     5821 cggagaatgg gtacatccag agcagccacc gcgacggcgc tgtgcaatcc cactgcctgg
     5881 tactggatca gccagtcgaa gaggcgcgtc tagcacattg gctggaactg atggcaaccc
     5941 tgcgcgggga aaacctgctg cgtatcaagg gcctcgtcaa ggtcgtcgaa caccccgatc
     6001 aacccaccgt ggtgcatggc gtacagcaaa tagtccaccc cttgcagcaa ctgccggcct
     6061 ggccctcaaa cgatacacgc tcaagaatcg ttctgattgt gatggacatc gactatgccg
     6121 aaatctcccg gaccttcgag cgttttgtcg gtgtacgcct acccgcattc aggcccctga
     6181 actcactcaa acaaatcagc ccgctgcagg ccccgatctg atgggcatgc acataccctt
     6241 aggtccttac gggcagggtt agctgaacgc cccgacatac cactgcttga cgttctaggg
     6301 atggtagata gactgctaat catgaacagg ttaacgaaaa tcctcccgta ttacagctac
     6361 cggctctcac ccagcgggcg gtagccgtgt gcataccgat ccgcctgagg cggatcggat
     6421 ccatgggatt tctgtctcct cgggcaccac caaccaggag acctccatga acaccccaca
     6481 agaccccatc cagttcctcc aggaacgtgg ctttatccac cagagcaccg atctcgaggg
     6541 cttgcgcgag cacctgcagc aaggctgcgc aaccatttac ctgggtttcg atgccaccgc
     6601 cgacagcctg catgtcggcc atttgcaggg cctgatggtg atgcgctggc agcaacgctt
     6661 tggccatcgc ccgatcctgc tgatcggcgg cgcgaccacg cgcatcggcg acccgagctt
     6721 tcgcgacgag agccggccga tgctcagcga ggcgcagatc cagcataata tcgtcggcat
     6781 ctgcgagacc ttcgcccgct tcgtgaatct gggcccatcc ggcaatgacg gcctggtggt
     6841 ggacaacgcc gactggctcg acggcatggg ttacctgcag tttctcaatc aggtcggccg
     6901 gcacttttcc atcaaccgcc tgctgacctt cgacgccgtg cgccagcgcc tggatcgcga
     6961 gcactcactg tcgttccagg agttcggcta caccctgctg caggcccatg acttcaccga
     7021 actggccaaa cgtcatggct gcagcgtgca gctcggtggc gcagaccagt gggccaatat
     7081 catcaacggc gtcgaactgg ccaggcgtca ggatggccgg caactgttcg gcctgaccat
     7141 gccgctactg accaccagcg acgggcgcaa gatgggcaag tccgcccagg gcgcgatctg
     7201 gctcaacccg cagcgcctct caccgttcga tttctggcag ttctggcgca actgcgacga
     7261 ccgtgatgtc tgccgctttc tggcgctgtt caccgagttg ccgctggctg aaattgccaa
     7321 cctggcccag gccgagggcg cgcaactgaa cgccgccaag gtacgcctgg cctccgccgt
     7381 gacggccctg gtgcatggcg aagaagccgc gcaactggcc gaagccaccg cccaggggct
     7441 gttcgcccag ggcggcgagc tgcaagggtt gaccaccctc agcgtgccca gatcccagct
     7501 cgccgcaggc tactccctga tcgagttgct ggagcagcat gagctgcaac cctcccgcgg
     7561 tgccactcgg cgtttggtcg ctggcggtgg cctgcgccta gatggcgaga cggtcgtcga
     7621 tgccgacctg gcgttgccct ccgacggtga tggctggctg ctcagcctgg gcaagaagca
     7681 ccactaccgc atacagctgg agggctgatc atcgagcatc cagagcagac ccagggcagc
     7741 ccactgaccg cctttgcccg ctggctcggt ctcgatgagg ccggcgcagc ccaggctgcc
     7801 cgtctggcgc tggcggcctg gctggcattc agcatcgccg ccctgctcca cgtgcagaat
     7861 gcctattggg ccgccatgcc catctgggtg gtggcacaac cctcccgcgg cctgctgctg
     7921 gaacgcgcgc tctatcgcct gctcggcacc ctgctcggcg cactggccgg cttcgtcgta
     7981 ctcacgcagg cctcatccct gctgcagctg atgctgctcg ccgcagtgat cgccctgtgc
     8041 gcgggcatga cccacctgct gcgcggcacc tcggcctatg cctggctgat ggccgggatg
     8101 accgctgcgg tggtggtcat cccctcggcc ctgagcccac agaacagttg gacgctggcc
     8161 ttggccaggg tcgagtgcac gctgatcggg gtactggcgg taactctggt gctgtggctg
     8221 ttcaccccca agacccgccg cgcagacttc tatcaccggg cccgagcgct ggcggcggat
     8281 gccgtcgact tcgcggccca aagcctgagc ggtggcaccg acccggccac cgaactgcgt
     8341 atcctcaagg cgcttagcga tatagacggc gcagcgcggc ccatctccgc cggttcgctg
     8401 cgcggttacc gccaccttcg ccatctcgac caactgctgc tggcctcgct caatatcatg
     8461 gccgccggcc aggccatggg ctgccacctg cgccccgaag cccaggccgc ggcagcgctg
     8521 gccagccgcc tgcaacaggt tgccgaacgg ctgcgcagcg aggagccggc cgacagtatc
     8581 ctgtccgcgc cgcctgccaa tctgccgccc agacgcctgg cccgcctcga gcaggccctg
     8641 gaacaactgt gccaggccgc agccca
//
LOCUS       DASRUM010000244         7698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1638244, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000244 DASRUM010000000
VERSION     DASRUM010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7698
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638244"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1069
                     /gene="rluD"
                     /locus_tag="VFF22_16125"
     CDS             95..1069
                     /gene="rluD"
                     /locus_tag="VFF22_16125"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HZX18380.1"
                     /translation="MSSINKQIIQLNAEVPYEMGGQRLDQVAALLFAEHSRSRLSAWI
                     KEGLLTVDGEVLRPRDIVHGGALLALNAEQEAQGEWIAQDIALNIVYEDEQILVIDKP
                     AGLVVHPAAGHADGTLLNALLHHVPDIINVPRAGIVHRLDKDTTGLMVVAKTIEAQTR
                     LVDQLQKRSVSRIYECIVIGVITSGGKINAPIGRSSSQRQRMAVSDGGKPAVSHYRVL
                     ERYRSHTHARVKLETGRTHQIRVHISHIGYPLVGDPVYGGRFRIPPAANPSMVQTLKE
                     FPRQALHARFLELDHPITGERMKWQSPLPDDFVWLLTLLRQDREAFIG"
     gene            1115..1840
                     /gene="pgeF"
                     /locus_tag="VFF22_16130"
     CDS             1115..1840
                     /gene="pgeF"
                     /locus_tag="VFF22_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HZX18381.1"
                     /translation="MHDWLTPQWPAPARVKTCVTTRSGGVSLAPYDSCNLGEHVGDDP
                     KAVLKNRQRLVSLLGCKPAWLQQVHGVAVVEANPALVLGADASWTGKPGIACTVMTAD
                     CLPALFCDRAGTRVAAAHAGWRGLAAGVLEATLDQLAVPADEVLVWLGPAIGPQAFEV
                     GAEVREAFIAQHVQAASAFQPSVNPGKFIADIYQLARIRLAAYGVEAVYGGDFCTFND
                     PRFFSYRRSAQTGRFASLIWLGN"
     gene            2006..4570
                     /gene="clpB"
                     /locus_tag="VFF22_16135"
     CDS             2006..4570
                     /gene="clpB"
                     /locus_tag="VFF22_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HZX18382.1"
                     /translation="MRIDRLTSKLQLALSDAQSLAVGLDHAAIEPLHLMQALLEQQGG
                     SIKPLLMQVGFDVNSLRLALGKELDQLGKIQNPSGDVNLSQDLARLLNQADRLSQQKG
                     DQFISSELVLLAAMDESTKLGKLLLGQGVSRKALENAVTNLRGGDAVNDPNVEENRQA
                     LDKYTVDLTKRAEEGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIAEG
                     LAQRIINGEVPDGLKDKRVLSLDMGALIAGAKFRGEFEERLKAVLNELSKQEGRIILF
                     IDELHTMVGAGKGEGSMDAGNMLKPALARGELHCVGATTLNEYRQYIEKDAALERRFQ
                     KVLVDEPSEEDTIAILRGLKERYEVHHKVAITDGAIIAAAKLSHRYITDRQLPDKAID
                     LIDEAASRIRMEIDSKPEVLDRLERRLIQLKVEAQALKKEDDEAAIKRLAKLNEDIAR
                     LEREYADLEEIWKSEKAEVQGSAQIQQKIEQSRQELEAARRKGDLNRMAELQYGIIPD
                     LERSLQMVDQHGKSENQLLRSKVTEEEIAEVVSKWTGIPVSKMLEGERDKLLKMEGLL
                     HQRVIGQNEAVVAVANAVRRSRAGLSDPNRPSGSFLFLGPTGVGKTELCKALAEFLFD
                     TEEALVRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVEKA
                     HPDVFNVLLQVLEDGRLTDSHGRTVDFKNTVIVMTSNLGSTQIQELVGDREAQRAAVM
                     DAVGTHFRPEFINRIDEVVIFEPLARDQIAGIAQIQLGRLRQRLAERELSLELSDEAL
                     DKLIAVGYDPVYGARPLKRAVQRWIENPLAQLILAGKFAPGTTVTGRVEGDEIVFG"
     gene            complement(4655..4990)
                     /locus_tag="VFF22_16140"
     CDS             complement(4655..4990)
                     /locus_tag="VFF22_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZX18383.1"
                     /translation="MDDERRLHSRHNADVQLEVFDLHTGQRLGRVVDLSADGFMLFSD
                     TPLTADELVECRLVSEQEVEGVCEITLGADCLWSRPGADGQHCWAGFHIIDLAEDQAA
                     ALEILLKHL"
     gene            complement(5000..7357)
                     /locus_tag="VFF22_16145"
     CDS             complement(5000..7357)
                     /locus_tag="VFF22_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 domain-containing protein"
                     /protein_id="HZX18384.1"
                     /translation="MSNQGIPPNPPGSTQTLASRGIQPRFGDLVQSCRKLVMNRLAEH
                     LTGVFAQVDDTLFECAEKAENNQVQTLFFDNMREIRRQRPQIERSYHQQIAQNFSDFL
                     EGKLKPPPSASELDAEHMALVQNEDYEESLQVTNMVSRVKARCAQPLFALEQRLALLN
                     NGQKLGEDINPFGPQMIAQALRDALTPCPFPLRIKVILYTLFDTHVMQSLDSIYAALN
                     QRLIDAGVLPNLKYTAQRNNPPPTRPKNTPTTPTEHAPESSASSKSQGTAAAMQTPGQ
                     TYNAGSTDLNGPPPSDPQELLGSLATLLGEHRQRDIDAPLLGGTRSIASFTPREATST
                     YSASALLDALNRMQQQSANDLAQRLSRPQQVEGLKADLQQQLEAHSELPGQQKVSDQE
                     ADVIDLVGMLFDFILDDDNLPDSCKTTLSHLHTPYLKVALQDKALFTQHHHPARRLLN
                     AMAQAGVLYGGEGDERGLLAKMQWVVERVIHDFFGDLQLFDGLLEEFNEFVATLRHKV
                     ELRERRAVEAAKGRDKLLGARQHAVEVIANSLHNRQPPTIIRNFLELTWADVLVFVLL
                     RNGDQSPEWQRACDAAEQLAWSGTLLDEAGRERLQKLRVPLLEDLRKGLELLGGYHED
                     GIRRLLQDLVACQHAVQAKQPQVAAQLKPTLPESPLGAMLGEDAALATLAPRESTLSP
                     RAQSLAKELGHIEFGTWFEFANGEQVRTLKLSWFSPTTRNYMFVDQSGQRVAIKPLTQ
                     LATEMEQGLARIVTPERGAPLVDRALTAIYRVLQRFTGRTAEPQE"
     gene            7571..7646
                     /locus_tag="VFF22_16150"
     tRNA            7571..7646
                     /locus_tag="VFF22_16150"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7604..7606,aa:Asn,seq:gtt)
BASE COUNT         1446 a         2181 c         2486 g         1585 t
ORIGIN      
        1 gatgagcgcc cccggtaccc ctggggcaaa gacgccgtat ttaaccacaa gcgtgtagcc
       61 gaaaccaaaa gctatgcccc ctctgttgcc gagcatgtcc tctattaata agcagattat
      121 tcaactgaac gccgaggtgc cctatgaaat gggcggtcaa cgcctcgatc aagtcgccgc
      181 actcctgttc gccgagcact cgcgttcgcg tctgtctgcc tggatcaaag agggtttgct
      241 gaccgtcgat ggtgaggtgt tgcgcccgcg cgacatcgtc cacggcggtg cactgctggc
      301 gttgaacgcc gagcaggagg cccaaggcga atggattgcc caggacatcg cgctaaacat
      361 cgtttatgaa gacgagcaga ttctggtcat cgacaagcct gctggcctgg ttgtgcatcc
      421 cgctgcaggg catgccgatg gcaccctgct caacgctttg ctgcaccacg tgccggacat
      481 tatcaatgtg ccccgcgctg gcattgttca tcgcctggac aaggacacca ccggcctgat
      541 ggtggtggcc aagaccatcg aagcgcagac ccgcctagtc gaccagctgc agaagcgctc
      601 ggtcagccgt atctacgagt gcatcgtgat cggcgtgatc acctccggcg gcaagatcaa
      661 tgcccccatc ggccgtagct cctcgcagcg ccagcgtatg gcggtcagcg atggcggcaa
      721 gcctgcggtc agtcattacc gggtgctgga gcgctatcgc tcgcataccc atgcgcgggt
      781 caagctggaa accggccgca cccaccagat ccgcgtgcac attagccata tcggctaccc
      841 gctggtaggc gacccggtct acggcgggcg tttccgcatt ccgccggccg ccaacccgag
      901 catggtgcag accctcaagg agttcccgcg acaggctttg catgcgcgct tccttgagct
      961 ggaccatccg attaccggtg agcggatgaa atggcagtcg ccattaccgg atgactttgt
     1021 ctggttgttg acgctgctgc gccaggaccg tgaagccttt atcggctgac aaatatccgg
     1081 aacacgagtg ggtaaccggg aattgggtga caggatgcac gactggctaa cgccgcaatg
     1141 gcccgcaccg gcaagagtaa aaacctgcgt tactactcgt tcaggcggcg tgagccttgc
     1201 gccttacgac agttgcaacc tgggcgagca tgtcggcgac gacccaaagg ccgtgctgaa
     1261 aaaccgtcaa cgcttggtca gcctgctcgg ttgcaagccg gcttggttgc agcaggtgca
     1321 tggtgtggcg gttgttgagg ccaatcctgc tttggtgctt ggagccgacg ccagctggac
     1381 cggcaaaccg ggcatcgctt gcacggtaat gactgccgac tgtctccccg cgctgttctg
     1441 tgaccgtgcc ggcactcgtg ttgcggcggc ccatgctggt tggcgtggcc tggctgccgg
     1501 agtgctggaa gccactcttg atcagttagc ggtgccggcg gacgaggtgc tggtctggtt
     1561 agggccggcg attggtccgc aggccttcga agtgggtgct gaggtgcgtg aggcctttat
     1621 cgcccagcat gtgcaagccg cgtcagcatt ccagcccagc gtcaatccgg gcaaatttat
     1681 cgccgatatc taccaactgg cgcgcattcg cctggccgcc tacggcgttg aagcggtgta
     1741 cggcggcgat ttttgcacct tcaacgaccc gcgtttcttc tcctaccgcc gttcggcgca
     1801 aaccgggcgc tttgccagcc tgatctggct gggtaactga tcctcacgtg ccgcgtctgc
     1861 ggcactgttg cggcaaatga tccaggtcaa cgccgcccgg cttgaaaccc gcacaatcgt
     1921 cctcatctac tcttcatctc gcaggctttc tgtttgggtg cgattcagtg cgccggcctg
     1981 ccaccaccac gagggttgat tgtttatgcg tattgatcgt ttaaccagca agttgcaact
     2041 cgcgctatcc gatgcgcagt ccctggccgt tggtctggac catgcggcca ttgagccgct
     2101 gcacctgatg caggcactgc ttgagcagca gggcggctcg atcaagccgc tgctgatgca
     2161 ggtcggtttt gatgtcaaca gcctgcgtct ggctttgggc aaggagctgg atcagctggg
     2221 caaaatccag aatccgtccg gcgacgtcaa tctatcgcag gatctggccc gcctgctcaa
     2281 tcaggccgat cgcctgtcgc agcagaaggg cgaccaattt atctccagcg agctggtgtt
     2341 gctcgccgcg atggacgaga gcaccaagct gggcaagttg ctgctggggc agggcgtcag
     2401 ccgcaaggcg ctggaaaacg ccgtgaccaa cctacgtggc ggcgatgcgg taaatgatcc
     2461 gaacgtcgag gaaaaccgcc aggccctgga taagtacacc gttgatctga ccaagcgtgc
     2521 cgaagaaggc aagctcgatc cggtgatcgg ccgtgacgat gagatccgcc gcaccatcca
     2581 ggtattgcag cgccgcacca agaacaaccc ggtgctgatc ggtgagccgg gtgtgggcaa
     2641 gactgcgatt gccgaaggct tggcgcagcg catcatcaac ggcgaagtgc cggatggcct
     2701 caaagataag cgtgtgctgt cgctggacat gggcgcgctg attgccgggg ccaagttccg
     2761 tggtgagttc gaggagcgcc tgaaagccgt gctcaatgag ctgagcaagc aggaagggcg
     2821 gatcatcctg tttatcgatg agctgcacac catggtcggc gccggtaagg gcgaaggttc
     2881 gatggacgcc ggcaatatgc tcaagccggc cctggcgcgc ggtgaactgc attgcgttgg
     2941 tgcgaccacg ctcaatgaat accgtcagta catcgaaaag gatgcggcgc tggagcggcg
     3001 cttccagaag gtgctggttg atgagccgag cgaagaagac accatcgcca tcctccgcgg
     3061 cctgaaagaa cgctacgagg tgcatcacaa ggtggctatt accgacggcg cgatcatcgc
     3121 cgcagccaag ctgtcgcacc gctatatcac tgaccgccaa ttgccggaca aggccatcga
     3181 cctgattgac gaggcggcta gtcgcatccg tatggagatc gactccaagc ctgaggtgct
     3241 agaccgcctg gagcggcggc tgattcagct taaagttgaa gcccaggcgc tgaagaagga
     3301 agacgacgag gcggcgatca agcgcctggc caaactcaac gaagacatcg ctcgcctgga
     3361 gcgtgagtac gctgacctgg aggaaatctg gaagtcggag aaggccgagg tgcagggttc
     3421 cgcgcagatt cagcagaaaa tcgagcagtc ccgccaggag ctggaagcgg cgcggcgcaa
     3481 gggtgatctc aatcgcatgg cggagctgca gtacgggatt attccggacc tggagcgcag
     3541 cctgcagatg gtcgatcagc acggcaagag cgaaaaccag ctgctgcgca gcaaggtcac
     3601 cgaggaagaa atcgccgaag tggtgtcgaa gtggaccggt attccggtga gcaaaatgct
     3661 cgaaggcgag cgcgacaagt tgctgaagat ggaaggtctg ctgcatcagc gggtcatcgg
     3721 tcagaacgaa gccgtggtcg cggtggccaa cgcagtgcgg cgctctcgtg ccgggctgtc
     3781 cgacccgaat cggccgagcg gttcgttcct ctttcttggt cctaccgggg tcggtaagac
     3841 cgaactgtgc aaggcgctgg ccgaatttct cttcgatacc gaggaggcgc tggtgcgtat
     3901 cgatatgtcc gagtttatgg agaagcattc cgtggcgcgg ctgatcggag cgccgccggg
     3961 ctatgtcggt tacgaggagg gtggctatct gaccgaggcc gtacgccgta aaccctattc
     4021 ggtgatcctg ctggatgagg tggagaaggc ccacccggat gtgttcaacg tgctgttgca
     4081 ggtgctggaa gatggccgcc tgaccgacag ccacggccgt accgtagact tcaagaacac
     4141 cgtgatcgtg atgacctcca acctcggctc cacgcagatt caggagctgg tcggtgatcg
     4201 cgaggcgcag cgtgcggcgg tgatggatgc ggtgggcacg cacttccgcc cggaatttat
     4261 caaccggatc gacgaggtgg tgatcttcga accgctggct cgcgatcaaa ttgccggtat
     4321 cgcacaaatc cagctgggcc gtttgcgtca gcgtctggct gaacgcgagc tgagcctgga
     4381 gttgtccgat gaggccttgg ataaactgat cgccgtcggc tatgacccgg tctacggcgc
     4441 gcgccctctc aagcgtgccg tgcagcgctg gatcgagaac cctctggcgc agctgattct
     4501 ggctggaaaa ttcgcccctg gcactactgt gacgggcagg gttgagggtg atgagatcgt
     4561 cttcggctaa gcgctgccgg tggcattaag gctgccgaat aaaaaacacc ccggttcagt
     4621 ttgcgctgac cggggtgttt tttatggagc tggattacag gtgcttgagt aggatttcca
     4681 gcgcggccgc ctgatcttcg gccaggtcaa tgatgtgaaa gcctgcccag caatgctggc
     4741 cgtcggcgcc agggcggctc cataggcagt cggcccccag ggtgatttcg caaacgccct
     4801 caacctcctg ctcactcacc aggcggcact ccaccaactc gtcggcggtc agcggggtgt
     4861 cgctgaacag cataaaacca tcggcggaca ggtcgaccac gcgacccagg cgctgcccgg
     4921 tgtgcaagtc gaagacttcc agctgcacgt cggcgttatg ccggctgtgc aggcgacgtt
     4981 cgtcatccat aggagttcct tattcttggg gctcagccgt gcgcccggta aagcgctgaa
     5041 gcacgcggta aatggcggta agggcgcggt ccaccagcgg tgcacctcgt tcaggggtga
     5101 cgatgcgcgc cagcccttgt tccatttctg tggccagctg ggtgagtggc ttgattgcca
     5161 cacgctggcc gctctggtcg acaaacatgt agttgcgggt tgttgggctg aaccaggaca
     5221 gcttgagtgt gcgcacctgc tcgccattgg cgaactcgaa ccaggtgccg aactcgatat
     5281 gccccagttc cttggccagc gactgggcgc ggggcgaaag tgtcgattcg cgcggcgcca
     5341 gggtggccag tgcggcgtct tcgccaagca tggcgcccaa tgggctctct ggcagcgttg
     5401 gtttgagttg ggctgccacc tgcggctgtt tagcctgtac ggcgtgctgg caggccacca
     5461 ggtcctgcag caggcggcgg atgccgtctt cgtggtagcc gccgagcagt tccaggcctt
     5521 tgcgcaggtc ttcgagcagc ggcacgcgca atttctgcag gcgttcacgg cctgcttcgt
     5581 ctagcagtgt gccgctccag gccagttgct cggccgcgtc acaggcgcgt tgccattctg
     5641 ggctctgatc gccattgcgc agcagcacaa ataccagcac gtcggcccag gtcagttcga
     5701 ggaagttgcg gatgatggtc ggtggctgac gattgtgcag gctattggcg atcacctcga
     5761 ccgcatgctg gcgcgcgccg agcagtttgt cgcgaccttt ggcagcttcc accgcgcgac
     5821 gttcgcgcag ctcgacttta tgcctgaggg tggccacgaa ttcgttgaac tcttccagca
     5881 ggccgtcaaa cagttgtagg tcaccgaaga agtcatggat aacccgctcg accacccact
     5941 gcatcttggc cagcaggccg cgttcatcgc cttcgccgcc atacagcacg ccggcctggg
     6001 ccatggcatt gagcaggcgg cgagcagggt gatggtgctg ggtaaacagc gctttgtcct
     6061 gcagcgccac tttaagatag ggcgtgtgca ggtgggatag ggtggttttg cagctgtcgg
     6121 gcaggttgtc gtcgtcgagg ataaagtcga acagcatgcc gaccaggtcg attacgtcag
     6181 cttcctggtc ggaaactttt tgctgccctg gcagctcgct gtgcgcttcc agctgttgtt
     6241 gcagatccgc cttgagacct tctacttgct gtgggcgact caggcgctgc gccaggtcgt
     6301 tggccgattg ctgctgcatg cggttgagtg catccagcag ggccgaggcg ctgtaggtgc
     6361 tggtcgcttc gcgcggggtg aagctggcaa tgctgcgggt gccgccaagc agcggcgcgt
     6421 caatgtcacg ttggcggtgt tcgcccagca aggtcgccag gctaccgagt aactcctgcg
     6481 ggtcactcgg tggtgggccg ttcaagtctg tgctgccagc gttgtaggtt tgcccaggtg
     6541 tttgcatggc agctgcagtg ccctggcttt tgctgctggc gctgctttct ggcgcatgtt
     6601 cggtcggtgt ggtgggcgtg ttcttggggc gtgtcggtgg cgggttgttg cgctgcgcgg
     6661 tgtacttcag attaggcagt acgccggcat caatcaggcg ctgattcagc gcggcataga
     6721 tgctgtccag gctctgcatg acgtgcgtgt caaacaacgt ataaaggatg acttttatac
     6781 gtagcggaaa cgggcagggc gtcagggcat cgcgaagggc ctgggcaatc atctgcgggc
     6841 cgaaggggtt gatgtcctcg ccgagcttct gaccgttatt cagcagcgcc aggcgctgtt
     6901 ccagggcaaa cagcggttgc gcgcagcgtg ctttgacccg gctgaccata ttggtgactt
     6961 gcaggctttc ttcataatcc tcgttctgca ccagagccat gtgctcggcg tccagttcgc
     7021 tggcgctggg cggcggcttg agtttgcctt cgaggaagtc ggagaagttc tgcgcgatct
     7081 gctggtggta gctgcgttct atctgagggc gctgcctgcg gatctcacgc atgttgtcga
     7141 agaacagggt ttgcacctga ttgttctcgg ctttttcggc gcactcaaag agtgtgtcgt
     7201 cgacttgggc gaagaccccg gtgaggtgtt cggccaggcg gttcatcacc agttttcgac
     7261 agctttgcac caggtcgccg aagcgcggct ggatgccgcg gctggcaagc gtctgcgttg
     7321 agccgggtgg gtttggcggt atgccctggt tgctcatgga ctttcctggc tggagcctta
     7381 cggctggtgc gtcctgcaat gaatacagac atagggaaga tatagcagcc gctcacgcag
     7441 ctgcaaatca ttgtactaaa agcaatttaa ggggttgaca ggcgcgcgcg gaaacgtaaa
     7501 atggcgcgcc tcagacacgg gaatcgattt aacaaccggc tcctgtttat gagaaaagga
     7561 agcaagtagt tccgcgatag ctcagtcggt agagcaaatg actgttaatc attgggtccc
     7621 aggttcgagt cctggtcgcg gagccaatct ttcatcgggg tatagcgcag tccggtagcg
     7681 cgcctgcttt gggagcag
//
LOCUS       DASRUM010000245         4974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1956905, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000245 DASRUM010000000
VERSION     DASRUM010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1956905"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1589
                     /locus_tag="VFF22_16155"
     CDS             <1..1589
                     /locus_tag="VFF22_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18385.1"
                     /translation="GNAPILGQYLAKIILNKDTQRFTNEDWKFIHSYLIDSCGIKANQ
                     SRLYFYIFSAGGGTGSGMASEFGLAQQFAYMSKTFDTKNFDESPENRDHSFVFEPIFT
                     SGICILPNISDQRSEMSEALHINAGRLLCKYLAEEWDFSYNFDNEENSEESVMRRIRP
                     WNAMMLISNDIMRYAEESDDGNIQNIDVNAMERHANQYISQQIFNILTAQAVTTDYDQ
                     NYFRRAGIDIGETIRLDANDLFMSLAGPVAVAYAESVVPEQSAAALDKFRVLDKEPSR
                     LNIDDLFFRSIDLPHFNKVTQAIEGISLLPIESKRYREALEEYKKSNYNAAKLSDLHF
                     FKNCSSVVSIVSLPKDYKLSYMDLNRLKTHLNSLFPNTTLKRYALVIGASANLSLTTL
                     IAKSPCLSDDFLTLIVAFIKRCFARDPHRFDESLDNAILDFIMSDKFDEARVDAMLNE
                     YENPAKILDTNWYAIKPMYEKKYRELIHDQDKFVSINDIRLSRDSVKKAIKYLREIYR
                     HRIGKTRVISLNKYTEDKPE"
     gene            complement(1638..2420)
                     /locus_tag="VFF22_16160"
     CDS             complement(1638..2420)
                     /locus_tag="VFF22_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZX18386.1"
                     /translation="MKIAANLSLLFTELPLIDRIVAAAAAGFDGVEIQFPYELPAVRL
                     KEALQAADMPLRLINLPAGDLMSGGPGLAAVPARQGEFDEALQQALTYAAMVRPALVN
                     VLPGCLADGVEPEQALACLIDNLRKTAEAFQLLGIGVVVEAINPLDMPGFLINTPAQL
                     DALLKALAHPNLAAQYDLYHMARQGLDVQAGIALLAGRIGHVQFADYPGRGAPGTGEL
                     QFAPLLTALRDSGYQGWLAAEYRPGEAGTQASLGWLEEWRAT"
     gene            complement(2417..3322)
                     /locus_tag="VFF22_16165"
     CDS             complement(2417..3322)
                     /locus_tag="VFF22_16165"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZX18387.1"
                     /translation="MSTSLPALAFAGIGLMGLPMTQRLLAAGYPLSVWNRSPDKCAPL
                     QGLGAQTVDSPAQLCARADIVMLCLANTEVVREVVYGPGGIVETAKPGQLLVDFSSLE
                     PAATREMAAELHARCGMHWVDAPVSGGTPGAANGTLAIMAGGRVEDVERVRPILAHLG
                     QRLTRMGDVGAGQVTKVCNQMIVACNALVIAEVVALAEQAGVDARLIAPALAGGFADS
                     KPLQILAPQMADSQFEPVKWHVRTLLKDLDTAVKLSQQVGSATPLSGLAAQLMRQHGS
                     QGNLQRDPATLVQLYLKPQHQESQP"
     gene            complement(3407..3775)
                     /locus_tag="VFF22_16170"
     CDS             complement(3407..3775)
                     /locus_tag="VFF22_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5064 family protein"
                     /protein_id="HZX18388.1"
                     /translation="MFEPGHLHRANTLATAETPLFSVDLHYEVRQDPTEGPMLHMRMQ
                     GQVDGKAFEEIFELHRDTAYNFASVATRLAHKHGLPTNSSLIMRSHAEYDLMFEDIRA
                     KLGAHSGEPVNLDHLEKDGL"
     gene            complement(3851..4168)
                     /locus_tag="VFF22_16175"
     CDS             complement(3851..4168)
                     /locus_tag="VFF22_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2288 domain-containing protein"
                     /protein_id="HZX18389.1"
                     /translation="MTEQPSTLYAKLLGETAAITWQELQPFFARGALLLVDGAQDLIE
                     VAQAVAQNDEGKVAAWLAAGTLSKADASRAEDLLNRDPELWAVVVAPWVLAQERIERA
                     PLH"
     gene            4462..>4974
                     /locus_tag="VFF22_16180"
     CDS             4462..>4974
                     /locus_tag="VFF22_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX18390.1"
                     /translation="MNKATKQISKLFAAMAMAGVASYSVAADTIKIGLAGPVTGAVAQ
                     YGEMQFIGAKMAIEQINKAGGVNGAMLEGVVYDDACDPKQAVAVANKIVNDEVKFVVG
                     HLCSSSTQPASDIYEDEGILMVTAASTSPDITARGYELVFRTIGLDSLQGPTAGNFIA
                     DHVKPKAVAVI"
BASE COUNT         1081 a         1630 c         1375 g          888 t
ORIGIN      
        1 ccggtaacgc gccgatcctc ggccagtacc tggccaagat aatcctcaac aaggacaccc
       61 agcgcttcac caacgaagac tggaagttta ttcacagcta cctgatcgac tcctgcggca
      121 tcaaggccaa ccagtcgcgc ctgtacttct acattttcag cgccggcggc ggcaccggct
      181 cgggcatggc ttcggagttc ggcctggcgc agcagttcgc ttatatgagc aagaccttcg
      241 acaccaagaa cttcgacgaa agcccggaga accgcgacca cagcttcgtc ttcgagccaa
      301 tcttcaccag cggcatctgc atcctgccga acatctccga ccagcgcagc gagatgtccg
      361 aggccctgca catcaacgcc ggccgcctgc tgtgcaagta cctcgccgaa gagtgggact
      421 tctcctacaa cttcgacaat gaagagaaca gcgaagaaag cgtgatgcgc cgcatccgcc
      481 catggaacgc catgatgctg atctcgaacg acatcatgcg ttacgccgaa gaaagcgatg
      541 acggcaatat ccagaacatc gatgtcaacg ccatggagcg tcacgccaac cagtacatct
      601 cgcagcagat cttcaacatc ctcaccgccc aggcggtgac taccgactac gaccagaact
      661 acttccgccg cgccggcatc gacatcggcg aaaccattcg cctggacgcc aacgacctgt
      721 ttatgagcct ggccggcccg gtggccgtgg cctatgccga atcagtggtg ccggagcaaa
      781 gcgccgccgc gctggacaag ttccgcgtgc tggacaagga gccgtcgcga ctgaatatcg
      841 acgacctgtt cttccgctcc atcgacctgc cgcacttcaa caaagtgacc caggctatcg
      901 agggcatcag cctgctgccg atcgagtcca agcgttaccg cgaagcgctg gaggaataca
      961 aaaaatccaa ttacaacgcc gcgaaactca gcgacctgca cttcttcaag aactgctcct
     1021 cggtggtgtc gatcgtctcg ctgccgaaag actacaagct gtcctatatg gacctgaacc
     1081 ggctcaagac ccacctcaac agcctgttcc ccaacaccac gctgaagcgc tatgcgctgg
     1141 tgatcggtgc ctcggccaac ctctcgctga ccaccctgat cgccaagagc ccgtgcctgt
     1201 cggacgattt cctgaccctg atcgtggcct ttatcaagcg ctgcttcgcc cgcgacccgc
     1261 accgcttcga tgagagcctg gacaacgcca ttctcgattt catcatgagc gataagtttg
     1321 acgaagcgcg ggtcgacgcc atgctcaacg aatacgaaaa cccagcgaag atcctcgaca
     1381 ccaactggta cgcgatcaag ccgatgtacg agaagaagta ccgcgagctg atccacgatc
     1441 aggacaagtt cgtctcgatc aacgacatcc gcctgtcacg cgacagcgtg aaaaaggcca
     1501 tcaagtacct gcgcgagatc taccgccacc gtatcggcaa aacccgggtt atctcgctta
     1561 ataagtacac cgaagacaag cccgagtaag ccacagcaac ctgcaacccg aggtcaaccg
     1621 ggttgcaggt taacgcctca agtcgctcgc cattcctcaa gccagcccag gctggcctgg
     1681 gtgccagcct ctcccggtcg atactcggcc gccagccagc cctgataacc actgtcacgc
     1741 aaagccgtca gcagcggcgc aaactgcagc tcgccagtgc ctggcgcacc gcgcccaggg
     1801 taatcggcaa actgcacatg gccgatacgc ccggccagca acgcgatacc cgcctgcaca
     1861 tccagcccct ggcgcgccat atggtagagg tcgtactgcg cagccagatt gggatgggcc
     1921 aaagccttga gcagggcatc cagctgcgcc ggggtgttga tcagaaagcc cggcatatcc
     1981 agcggattga tcgcctccac caccacacca atgcccaata gctgaaaggc ctcggcggtc
     2041 ttgcgtaggt tgtcgatcaa acaggccagc gcctgctcag gctcaacgcc gtcagccaga
     2101 catccgggca gcacattgac tagcgccggt cgcaccatgg cggcgtaagt cagcgcctgc
     2161 tgcagcgcct catcaaactc gccctgtcgc gcaggcacgg cagccagtcc gggtccgccg
     2221 ctcatcaggt caccggccgg caggttgatc aggcgcagcg gcatgtcggc agcctgtaac
     2281 gcctctttta ggcgaacggc cggcaactcg tagggaaact ggatttccac gccatcaaag
     2341 cccgcagcgg cggcggccac tattcggtcg atcagcggta actcggtaaa cagcagcgac
     2401 aggttggccg cgatcttcat ggctgtgact cttgatgctg tggcttcaga tacagctgca
     2461 ccagggtcgc cgggtcacgc tgcagattgc cctggctgcc gtgctgacgc atcagttgcg
     2521 cggccagacc gctgagcggc gtggccgagc cgacttgctg cgacagtttg accgccgtat
     2581 cgaggtcctt aagcagagtg cgcacatgcc atttgaccgg ttcaaactgg ctatcggcca
     2641 tctgcggcgc aagaatctgc agcggcttgg agtcggcaaa accgccggcc agcgctggcg
     2701 cgatcaggcg ggcatcgacc ccagcctgct cagccagcgc caccacttcg gcgattacca
     2761 gcgcattaca agcaacgatc atttggttgc acaccttggt cacctggccc gcgccaacat
     2821 cgcccatgcg cgtcagacgt tgccccagat gcgcgagaat cggccgcacc cgctcgacat
     2881 cctcaacccg gccaccggcc atgatcgcca gcgtgccatt ggcagcgccc ggtgtgccgc
     2941 cagagacagg cgcatcgacc caatgcatgc cgcaacgagc atgcagttca gcggccattt
     3001 cgcgagtggc cgccggctcc aggctggaga agtccaccag cagctggcca ggtttggccg
     3061 tttccacaat gccgcctggg ccatacacca cctcgcgcac cacctcggtg ttggccaggc
     3121 aaagcatcac gatatctgcc cgagcacaga gctgcgccgg gctgtccacc gtttgcgcgc
     3181 ccaacccctg cagcggtgcg cacttatcag gggaacggtt ccacacactc agcggataac
     3241 cggcagccag caggcgctgg gtcatgggca ggcccatcag gccaatacca gcaaatgcca
     3301 aagcaggaag ggaggtggac ataggcagac tccgactcat tgatggccgc catcttaacc
     3361 tagcggcgcc ggaacctgtc ccgccagtca acgcccgagc aacgacctac aggccgtctt
     3421 tttccagatg atcgagattg accggctcgc cgctgtgcgc accgagcttg gcgcgaatgt
     3481 cttcgaacat caggtcgtac tcggcatggc tgcgcatgat caggctgctg ttggtcggca
     3541 ggccatgctt gtgtgccaag cgcgtggcca cgctggcgaa gttgtaggca gtatcacggt
     3601 gcagctcgaa tatctcttca aacgccttgc cgtccacctg cccctgcatg cgcatatgca
     3661 acatcgggcc ttcggtcggg tcctggcgta cttcataatg aaggtcgacg ctgaacagcg
     3721 gcgtctcggc cgtggccaag gtattggcgc gatgcaaatg tccaggttca aacatgttgg
     3781 cagctcccgc aatcagttgg tcgtgctgcc agcatagcta cccaggatga tgcgtggtgc
     3841 atgcgccaga tcagtgcaac ggcgcgcgtt cgatgcgttc ctgcgccagc acccagggcg
     3901 ccacgaccac cgcccacagc tctggatcgc ggtttagcag gtcttcggcg cggctggcgt
     3961 cagccttgct caaggtacct gcggccagcc aggcggcgac tttgccctca tcgttctgcg
     4021 ccacggcctg cgccacttcg atcagatcct gggcgccgtc gaccagcagc aaggcgccac
     4081 gggcgaagaa aggttgcagc tcctgccagg taatagctgc agtttcacca agcagcttgg
     4141 catagagggt gctaggttgt tctgtcatgg atggccgctt ttttaaccgg ccgccatgat
     4201 agcgccgacc agcccgctgg caaattcgca acgctgttcg cgacgttgcc aacggcgatc
     4261 aagacacgcc gtttttctat acgtttattg cgttaaagcg acaccccagg gttttgcccc
     4321 cagcaagccg cttttcaagc cgcaaagctg cgctctacac tgcaccggta cagttgccgg
     4381 gtggatgacc ggggaataac ccggttctgc agctttggcc gccagaacta caacaatcaa
     4441 aaccaataag agtggagcac tatgaacaag gctactaagc agatttccaa actgtttgcc
     4501 gccatggcca tggctggcgt cgccagctac tcggtagcgg ctgacaccat caaaattggc
     4561 ctggcaggcc cagtcaccgg cgccgtcgcg caatacggcg aaatgcagtt catcggtgcc
     4621 aaaatggcta tcgagcagat caacaaggcc ggcggggtaa acggtgcaat gctcgagggc
     4681 gtggtgtacg acgacgcctg cgatccgaag caagcggttg ccgttgctaa caagatcgtc
     4741 aacgacgaag ttaaattcgt agttggccac ctctgctcca gctccactca gccagcgtct
     4801 gacatctacg aagacgaagg catcctgatg gttaccgcgg cttccaccag cccggacatc
     4861 accgctcgcg gttacgagct ggtgttccgc accatcggtc tggacagcct gcaaggccca
     4921 accgctggca acttcatcgc tgaccacgtc aagccgaaag cggttgccgt gatc
//
LOCUS       DASRUM010000246        40695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_781245, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000246 DASRUM010000000
VERSION     DASRUM010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 40695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40695
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781245"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..319
                     /locus_tag="VFF22_16185"
                     /pseudo
     CDS             1..319
                     /locus_tag="VFF22_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029308938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1275 family protein"
     gene            complement(368..1123)
                     /gene="cobM"
                     /locus_tag="VFF22_16190"
     CDS             complement(368..1123)
                     /gene="cobM"
                     /locus_tag="VFF22_16190"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012020314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HZX18391.1"
                     /translation="MTVYFIGAGPGDPELITLKGQRLLCSCPVILYAGSLVPAAVLEG
                     HSAEQVINTAELHLDEIIELIRQAHDKGQDVARVHSGDPSLYGAIGEQIRHLRRLGIA
                     FEIIPGVTATAACAALLESELTLPEVSQTLILTRYASKSNMPAGEALGDLARHGATMA
                     IHLGVVHLSKIVTELLPHYGADCPIAVVHRASWPDQDWVSGTLATIEEQVRAKGFRRT
                     ALILVGRVLHAEQFADSALYNAEHRHLFRSAED"
     gene            complement(1120..1563)
                     /locus_tag="VFF22_16195"
     CDS             complement(1120..1563)
                     /locus_tag="VFF22_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HZX18392.1"
                     /translation="MIPSNASRRPAAAIQVVAGLGCRSGCNVEDLFSLLLNSLQALDL
                     AVDNLAGLASIAHKRDEPGLLALAERLGLQLSCFSAAELQPYQHDIQGSPLTLAATGS
                     PAVAEPCALALAERLSGNAARLLGEKTRNASATCALASIAPKDVS"
     gene            1783..2301
                     /locus_tag="VFF22_16200"
     CDS             1783..2301
                     /locus_tag="VFF22_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020293976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZX18393.1"
                     /translation="MSLPETDSIISLDVLYGTHRGWLHGWLRRSVGCSQQAADLVQDT
                     FVRLLVRGQPINNRAPRALLTRIARALVIDHWRRDALERAYLEALAQVPESSYPSPEV
                     RHEALQSLERIAQMLDGLKPAVRQAFLLYQLGEMSHAQVAQQLGVSSRTVERHVASAL
                     LHCYRLCFEVEQ"
     gene            2298..3224
                     /locus_tag="VFF22_16205"
     CDS             2298..3224
                     /locus_tag="VFF22_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020293977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4880 domain-containing protein"
                     /protein_id="HZX18394.1"
                     /translation="MSAAERLPEPIVCTAIEWQMRLRAHADSAELRRQLQDWLNQDEL
                     HQQAWQRLQQMAGLFQVSHLPAARETIPLLQRAEADLSRRRMLKLLGFGLTAGSATLL
                     ATSTPPAWRADFATATGESRRVRLGDGTQVQLNTGSALDVQGQQLLLRAGEVLVEGAQ
                     WQLQCRFAECRGRQARVLVRERDGYSEVRVERGEVLVATEAGQRWLQAGEGLGVSATT
                     MSELGKGPLDPFAWARGLLLVNDIRLADFLAEAGRYRQGWLGYDPAVADLRLSGVFRL
                     DQPELMLRNITHLLPVAIVERTRWWVRIVPVA"
     gene            3290..5665
                     /locus_tag="VFF22_16210"
     CDS             3290..5665
                     /locus_tag="VFF22_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009407331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HZX18395.1"
                     /translation="MRSARPLPFQLQPRILSAAVCLGLALFAGTASLSTHAAEPAASV
                     AAPNVPAGPLDQTLSLFAEHAGIILSYSPDMVRGLSSRGVAGGGSLKEGLAQLLGGTG
                     LVAEKTASGYVIRLIKADDSYQLEPMTINATATQSAFAPVPGYFASNASSATKSDKPI
                     LETAQSISVVTAEQMADRKVNRVEDAVAYTAGVRVGGSGLDPRFDTINVRGFETTQSA
                     DFLDGLRQAGSGWLALPSIEAYSLERIEVLKGPASVLYGQISPGGMVNRVSKRPSLLA
                     KNQVEVQAGNYNHRQGQFDIGGKLDEAGDVLVRTVGIYRDAEYSIEQMDNNTRLLAPS
                     LSWQIDPDTSLTLLAQYQERQTAASPMLYRDGDHLTNTWQGDEYFDKLEQRKWTVGYE
                     FEHAFNETFSLQQNLRYGQLDTTNQYLDVGVASVDKILPRSTAGSYEDMSTLSTDTRL
                     ISRFATGNLQHTLVSGVDYAWLDTELVYASGTAPSLDLNAPDYHQPISDPDNVLVDRE
                     GLEHRAGMYLQDQIEIDRWRLSAGLRRDWVHARTNGYVWGEDVNTKISNSATTGSVGA
                     LYLFDNGLAPYASYATSFLPQSGAEFGGKLFEPSEGEQYEVGLKYQPLGSSTMLTASL
                     YHLAQSNVSTRDPNNASFQVQTGEQVSRGLELEAVSDLTDSLRMSASYSFNDAEVTKD
                     SDFKGKAPKNTPRHLTSLWLNYSLPFGLGVSAGARYTGSTYGNGENTAKNEDYTLFDA
                     GVHYDFGSSLDGVRLALNARNLTDKRYINCQDGYCYRGEARSVVTSLSYTW"
     gene            5662..6510
                     /locus_tag="VFF22_16215"
     CDS             5662..6510
                     /locus_tag="VFF22_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009407330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZX18396.1"
                     /translation="MSAVNWLRSAATALVLLGGVNVAQAEPVALDGTEQWAMQSAEGR
                     NYRIMISLPEGDVPYTGGYPVIYMVDGNALFPAFHAAKRAQERWKGSILVAIGYPSDT
                     PYDFNRRAFDLSPPQLPERNKPPQGGQDLFLDFIEQRLMPKVNARFKVDQDQRSLVGW
                     SFGGMFGIYTLFTRPALFQHVVAISPSLWWRDRYLLAHERAFTEQAHAGKLDLTHSSL
                     TMLTGDREMPQEIQDVRSLQLRMENLSQYGLRSDFQIEAGEDHMSIPFRATTRVLEEL
                     LTTRRY"
     regulatory      6573..6768
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6868..7923
                     /gene="cobW"
                     /locus_tag="VFF22_16220"
     CDS             6868..7923
                     /gene="cobW"
                     /locus_tag="VFF22_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005737828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobW"
                     /protein_id="HZX18397.1"
                     /translation="MQTLAKLPVTIVTGFLGAGKTTLLRHMLGHADGRRIAVIVNEFG
                     ELGIDGEILKQCSIGCSEEEANGRIFELANGCLCCTVQEEFFPVMRELVARRGELDHI
                     LIETSGLALPKPLVQAFNWPEIRNACTVDAVITVVDSPAVAAGTFAAFPDQVDAQRKL
                     DPNLDHESPLHELFADQLASADLVILNKADLLDAAALAAVRAEVAEELPPAVKIIEAH
                     GGELPLDVLLGLNCETELHIEGRKTHHDLEGHEDHDHDEFASFHVELPEAEEARLLQA
                     LKEAVSKHGILRIKGFAAIPGKPMRLLLQGVGQRFDKHFDRAWQADEARITRLVVIGQ
                     TLDQAAIEAELNAALAG"
     gene            7930..11664
                     /gene="cobN"
                     /locus_tag="VFF22_16225"
     CDS             7930..11664
                     /gene="cobN"
                     /locus_tag="VFF22_16225"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HZX18398.1"
                     /translation="MHLLRTQPGSQLPVDSIADLGQTPAELVILCTGDSHLSLLAEVA
                     RQLPADYPSLRLASPAQLSNHASVDLYVEQVLQHAKVILISVHGGVSYWRYGIERLVE
                     LAERGARVIMVPGCDNPDPELMALSNVPVAEAERLWQFLRQGGAGNALELFNCIASQW
                     LQREYSWVEPQPLPRVGLYHPHLANPSLADWQANWQVDAPVAALLFYRTQVQAANTGF
                     IDVFCQRLQAQGLNPLPIAVASLKEAACLDQVEDWLEQTEAALIINTTAFALSNPEAP
                     SARPFRRDIPVLQAICALDNHEQWQANAQGLGSRDLAMHIVLPELDGRLITQPISFKG
                     LAWRSERSQSDVVCYQPHLPGMDFVAELARNWIALAHKSNADKRIALILANYPTRDGR
                     IGNGVGLDTPAAALNILKALQQQGYPVEELPDSGTALIHQLLGGVTNDLDMLDARPCA
                     QSLALDEYLAFFHSLPAANQQAVRERWGEPQQDSMFRSGRLMIAGLRFGLTFVGIQPA
                     RGYQLDAAAVYHDPDLVPPHGYLAFYCWLRKAFACDAVIHVGKHGNLEWLPGKSVGLS
                     EQCWPSAILGPLPNIYPFIVNDPGEGAQAKRRTQAVIIDHLMPPLTRAESYGPLRDLE
                     RLADEYYEASQLDLRRAAELRGEILLKVREASLDRELGLQLNADPNSWLPQLDAYLCD
                     LKESQIRDGLHVFGESPAGTLRRDTLLALLRIPRGDGQGGNASLLRALARDLELDFDP
                     LDCDMAAPWLGLRPVVLQSLSDDSWRSAGDTRERLELLALQLIGATDFESVGIESTQV
                     LQRLREQIAPLLDACGPAEMHGLLAALSGRFVPAGPSGAPSRGRLDVLPTGRNFYSVD
                     VRNLPTPTAWRIGVQAADRLLERHQQDHGDHLRQLGLSMWGTATMRTGGDDMAQALAL
                     MGVRPVWQAGSQRVERFEVLPLAQLGRPRVDVTLRVSGFFRDAFSNLIRLFDDAVQAV
                     VDLDEPEEMNPLSARVWRESLTLQDSGLDEQEARKQAGWRVFGSKPGAYGAGVQNAIE
                     ERLWQTREDLAEVYLNWGGYAYGTHSEGTPARAQFAERLEQMQAVLHNQDNREHDILD
                     SNDYYQFQGGMLAAVETLRGAKVASYHGDNSQPDTPRIRTLKEELNRVVRARAANPKW
                     IDGMKRHGYKGAFELAATIDYLFAFDATSELVDDHQYALLTDAYLLDKSTRDFIQQHN
                     PGALQDILERLLEAQQRGLWQHPGEYREALENLLIDSEEA"
     gene            11900..12913
                     /locus_tag="VFF22_16230"
     CDS             11900..12913
                     /locus_tag="VFF22_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX18399.1"
                     /translation="MTEHHFPLAAVVAADDLKLALCLAAIDPAIGGVLIEGPRGMAKS
                     TLARGLADLLTSGTFVTLPLGASEERIVGTLDLDTALGEGRTQFSPGLLAKANGGVLY
                     VDEVNLLPDHLVDLLLDAAASGVNHIERDGISHRHAARFVLIGTMNGEEGELRPQLLD
                     RFGLNLALNAQPQPAQRAEIVRRRLAFDADPQAFLQRWQAQQDALRERCQNARARLAA
                     IPLDDAALEQISQRCFAAAVDGLRADLVWLRAARAHAAWRGAEHIESVDIDAVADFVL
                     RHRRRHNSPPAAQPPQPQSQSQPSNDKPSEQPQGEGQWGELPAQAQSLGVRREPPRWA
                     KKP"
     gene            12916..13602
                     /locus_tag="VFF22_16235"
     CDS             12916..13602
                     /locus_tag="VFF22_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZX18400.1"
                     /translation="MRPRTAPGADARAAPGTLGGGRNGASRSGAAGRIDWPASLLKGR
                     PRCRQDLVLRPRSAKPTELWLVIVDASASTRRHGALSKAKGLLSEVFEQARRQRARLA
                     LLHATGRQAQWLWQGQKASHALQQWLAELGAGGGTPLLAAVQQAADWQARRQRLHPGE
                     HQRLLIITDGRLRDWPTLTPSVCPALLVDIESAPIRLGRGRQLAAELGADYRHIDSLP
                     LLAGNLETAL"
     gene            13599..14366
                     /gene="cobF"
                     /locus_tag="VFF22_16240"
     CDS             13599..14366
                     /gene="cobF"
                     /locus_tag="VFF22_16240"
                     /EC_number="2.1.1.152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043819 - precorrin-6A synthase
                     (deacetylating) activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A synthase (deacetylating)"
                     /protein_id="HZX18401.1"
                     /translation="MKNLLIIGIGAGDPDYLTVQAINALNRTDVFFLMDKGSTKDSLI
                     GLRKTICERFIQGRDYRFASADCPERVRDVPDYRGSVVDLNQDKQQVFEGMIREQMAD
                     GETGAFLVWGDPALYDSTLRIVEQIVANSSEQIAYEVIPGITSLQALAAKHKVCFNSI
                     GRAFQITPARRLAEAGFPEGLDSVLVMLDAQDTYQRFVDQPMQIYWGAYIGTPDEVLI
                     AGPLSEVAETIRTRRAELREQHGWIMDSYLLRRSGTG"
     gene            14571..14963
                     /locus_tag="VFF22_16245"
     CDS             14571..14963
                     /locus_tag="VFF22_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZX18402.1"
                     /translation="MFSHICIGVSSFPRALAFYQPIMALLGIELRFLDEHKPWAAWQS
                     KPEPRPLLLIGKPHDGAPHHPGNGQMVALLAQNRAQVEQAHRLALAHGGHCEGPPGLR
                     AEYHPDYYGAYFRDPDGNKLCVVCHQPE"
     gene            15060..15236
                     /locus_tag="VFF22_16250"
                     /pseudo
     CDS             15060..15236
                     /locus_tag="VFF22_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012020308.1"
                     /note="catalyzes the formation of precorrin 6x from
                     precorrin 5; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A synthase (deacetylating)"
     gene            15371..16615
                     /locus_tag="VFF22_16255"
     CDS             15371..16615
                     /locus_tag="VFF22_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18403.1"
                     /translation="MAAKWKKKSKPELVIDRIKKHASINDKGILSFAGFEFFQYEAAL
                     HAIIDFGENISFKTKKELRDKSLRACLRNGSLTAKDFINTINKEISIYKQKQDSVFVL
                     TTSVSLSGGLPISKIVLPDAVVQSYSKGLPKKYKNRSAHDNKWLELFSDSPLPPLYSH
                     VAVRVNAKRPSDAFDIAVNSLDFIRGVVGLNVNSSYQISLGTGLSKPLNKVMLGGMHC
                     LHFENGELVFPDFFWYERHYKERSVTDINERRKYIAKGCRFLLGSLSKYPKEDFDLLR
                     NAIARYARAFDEYDRDVLIQKMWAALESIVASGENNADVIVRRCSYLYSDREYASQVL
                     EYIRDYRNQNVHRGHSEEGLDHYCFELQKFFRELVIFYARFSRLFTSLSEANRFLDCS
                     DNERKLEREIEILTRAKKFRFG"
     gene            complement(16709..18370)
                     /gene="cobJ"
                     /locus_tag="VFF22_16260"
     CDS             complement(16709..18370)
                     /gene="cobJ"
                     /locus_tag="VFF22_16260"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HZX18404.1"
                     /translation="MTSAPAIIILGPSALPCARRIQALYPQAEIHGLRGRVADVERIY
                     EDFGDTLRTLYRAGTPLIVLCAAGIVIRSLAAVLGEKDSEPPVLAVAEDGSAVVPLLG
                     GLGGVNRMAREIAAHLQVSAAITTSGELRFGTCLLEPPAGYVLADLEQGKGFVSDLLG
                     GQAVRIEGDAPWLAQAKLPVDNQANRVIHISPHNRAANADELLIHPQQVAVWVERADA
                     ELLSELQQALHSSELAPQSLACLLAAPELMANTELHHTAAQLKLPLRFIDDASQLPPL
                     HSQHANLRLLLAAAPIDATQLGRPRGRLTVIGLGPGAAEFMVPAARQALDEAQDLLGY
                     ETYINMAGPLRPEQVRHCTDNREEMQRARHAFELAASGRRVVVVSSGDPGVFAMAAAV
                     LEALHESADAEWQRVDLQVFPGVSAALATAAKAGAPLGHDFCLISLSDNLKPWTIIEK
                     RLAHAAAADLVMAFYNPISKARPWQLGSALEIIRQQRTPETLVVLGRDIGRPGETLRT
                     LTLGELTPEMVDMRTLVIIGSSQTCRFPRAEGGEWVYTPRSYPQM"
     gene            complement(18600..19352)
                     /locus_tag="VFF22_16265"
     CDS             complement(18600..19352)
                     /locus_tag="VFF22_16265"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HZX18405.1"
                     /translation="MMSVQKGRLLGIGVGPGDPELITLKALRLLQSAAVVGYFVAKAK
                     ANKGQGGNAFGIIEQHLTDTQQRLPLVYPVTTEKLAAPLSYEDVISDFYDTCAVQIAA
                     RLDAGHDVAVICEGDPFFYGSYMYLHDRLADRYDVEVVPGVCSMLGCASVLGTPLVYR
                     NQSLSVLSGVLPEDELKQRLHSAEAAVVMKLGRNFEKVRRVLQELGIDQRAHYVERAT
                     MGNQRIVPLDEVEPMSSPYFSMIVIPGEKWRG"
     gene            complement(19349..19975)
                     /locus_tag="VFF22_16270"
     CDS             complement(19349..19975)
                     /locus_tag="VFF22_16270"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HZX18406.1"
                     /translation="MLEYIRDGQEIYRQSFATIRAEADLSAIPADLEKLAVRVIHACG
                     MVDVVQDLRFSPGAGAAGRAALAKGAAILCDARMVAEGITRPRLAANNPVICTLHNDG
                     VIEQARALGNTRSAVALEHWREHLEGSVVVIGNAPTALFYLLEMLDAGAPKPALIIGM
                     PVGFIGAAESKDALAADSRGVPYVIVRGRRGGSAMAVAAVNALATEVE"
     gene            complement(19968..21311)
                     /gene="cobG"
                     /locus_tag="VFF22_16275"
     CDS             complement(19968..21311)
                     /gene="cobG"
                     /locus_tag="VFF22_16275"
                     /EC_number="1.14.13.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0019250 - aerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B synthase"
                     /protein_id="HZX18407.1"
                     /translation="MRDRPTSADLPDPRPSACPGLLRIVPALDGGICRVKLAGGVLSS
                     QQARAIAEAAERCASGVLELTNRSNLQIRGVLPVQQAELIERLLAAGLGPSNPAADDV
                     RNLLLSPAAGLDPQALLDTRPLAAALLDLLQTTPALHGLSAKFALQLDGGEALAMLEH
                     PHDIWLSALPGSPARLTFGLAGCPTDTPLGVVDVEQAVPLVEQLLRLFLDLAGSEHSR
                     MRQLLSVIPAHQLLQQLQTRLPFTVQAPPTDWQHKPVSPRAPMGIYPQQQNALCIVAA
                     ASRLGRIDAVQLNALAGLAEHYGDASLRLTPWQGLLLPNIPEHSADNLLHALGELGLL
                     IDAQEPLSNLIACTGSAACGKGLADSKADAMRLAERLRASTARPQVHLSACPRSCAAA
                     HTAPFTLLASSAGHYQLYQRTPEAAGFGQLQASAMTIDEAGDWFAAHCATGNSDA"
     regulatory      complement(21407..21608)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            21667..22869
                     /gene="cbiE"
                     /locus_tag="VFF22_16280"
     CDS             21667..22869
                     /gene="cbiE"
                     /locus_tag="VFF22_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012020295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HZX18408.1"
                     /translation="MTAWLTLVGIGEDGYAGLGEAARQALGEAQWIVGSPRQLALLPS
                     DLPGQHERWPSPFSLEPVMARRGMPVCVLASGDPLFYGVGASLARQLPAEELRVFSGP
                     SSVSLAAARLGWPLQEVTVLSLVARPLAALQAQIFPGARLLLLSNDGDSPAAVAELLR
                     ARGFGPSRLTVLEHLGGDQERRIDGLASDWAVPHVADLNLLAIECVAAADARLLPLTP
                     GLPDDAYQHDGQLTKRDVRAITLARLAPQPGELLWDVGAGCGSIGIEWMRTHPSCRAL
                     AIETDAGRQAHIQHNRDALGVPGLQLVAGRAPEALAGLAAPDAIFIGGGVTVLGVLEQ
                     CWASLKPGGRLVANAVTLQSEAALVAWREQVGGELTRISVAQAQPLGGFDTWRSALPI
                     TLLEVRKP"
     gene            22821..23585
                     /locus_tag="VFF22_16285"
     CDS             22821..23585
                     /locus_tag="VFF22_16285"
                     /EC_number="1.3.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-6A reductase"
                     /protein_id="HZX18409.1"
                     /translation="MAQRLADHPAGGAQAVSPRILLLGGTTEALRLARRLSTEAIYSL
                     AGLGRVPDDLPCQVRVGGFGGAEGLATFIREQGVELLLDLTHPYAAQISANAARACEL
                     SGIACWALRRPGWQAGAGDDWREVDNWTGLVAALNDFQRPLFTLGREPLEHLHEIPLH
                     QHWTVRCLTAQQGVPRAEIIGARGPFALDEERALFARLGTDVLISKNSGSQSTEPKLQ
                     VARELGLPVLLLRRPLLPSVDREFVSLEALTEALQL"
     gene            23663..24379
                     /locus_tag="VFF22_16290"
     CDS             23663..24379
                     /locus_tag="VFF22_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HZX18410.1"
                     /translation="MTTPVSPYARILFVGPYLTEGSFAELFRRRLTELRGAAALADIV
                     DTSEGYAPLWQRVAEGERPLLVIDLEPQSSSPHLDWLRSELCQQANPEQLFVASAIGQ
                     AEGLPVEAACALIERPELHLPCVGVAAVPGHHAWSQIPPHAQRLLLCNGPRCTRRGAL
                     DLWKTLRQRLKAAGKLECEGGVHITRTQCQFPCDLGPTASLYPAGEWFRVRDEAELIR
                     LVDERLVAGRAVPELRIDQS"
     regulatory      24525..24730
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            24885..26996
                     /locus_tag="VFF22_16295"
     CDS             24885..26996
                     /locus_tag="VFF22_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012271712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZX18411.1"
                     /translation="MARYPLSQLSVSLALCLVGTAVAGESPVNLPATTVQAKAAAEGE
                     SDLHMPSSSGSRLGLTALQTPASTSSLSGAQVRGRNNLTVQDAVTRTPGISSIGSPGN
                     GGTALSARGFTGHSATMQLYDGTRQYVGAGTVTFPVDTWSVERVDVLRGPASVLYGEG
                     ATGAVINVVPKKPFSGAISNQLRLGYGSDDRRQAALDSGGSLSDALSYRLNLNQQASN
                     GWVDGGESQSQALSAALRWDAGDALSFTLSHDQGDQDPQRYLGTPLVAGQYRESLRER
                     NYNVDNAEIRYNDQITRLVSDWRINDALTASNQLYYIKTQRYWRNAESYRWQPGDQVK
                     RSDFYEIKHTQEQVGDRQTFTLEHALFGLDSRTVMGVDYNRIHFARQHDFSSSFTDSV
                     PLAGSGGGQYQSTDPLRYGPRERNLARQFSLFAENRTQLTERLSLVSGVRRDQLHIQR
                     DNLLTDSSADRSLAGDNWRAGLVFEVTPELSLYGQYATSTEGVSNLLTLNPSQQQFDL
                     SEAQQTEIGLKQAFWDGQGEWTLAAYHIVKEKLLSRATPTAPTEQIGQQSSDGLEATL
                     ELALGQGWQVSANAALVRAEYDEFREAGGDRSGNRPTNVPRRTANLWLSKALSEQVQA
                     GIGARYVDSRYADTANSVALPSYTVVDANIGWQVQPAVRLGLELNNLFDRQYAITGSS
                     DGQQWYLGAPRSVFVTADYRF"
     gene            26996..27766
                     /locus_tag="VFF22_16300"
     CDS             26996..27766
                     /locus_tag="VFF22_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX18412.1"
                     /translation="MARLQISDLAWSPNGDQALLDGIELVVGDGQLVGLLGPNGSGKT
                     SLLRCAYRFQRPDQGSVELDGEALWSRSPRWCAQRVAVVLQEFPQDFGLTVAEVAAMG
                     RTPHKGLFDGDDQADVALVEQALARVGLTEHKRQAFAHLSGGEKQRVLLARALVQQPT
                     LLILDEPTNHLDPRYQLELLRLIKTLGLATLASFHDLNLAAAFCDRLYVLDHGRVVAS
                     GTPTEVLTEALLAEVYGVQALVDRHPLAGHPRITWINP"
     gene            27763..28716
                     /locus_tag="VFF22_16305"
     CDS             27763..28716
                     /locus_tag="VFF22_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011534224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX18413.1"
                     /translation="MKRFLRYVLCLPLLGVALSAQAVTVTSCDRQLTIEQPPQRAVSQ
                     DINLTGMLLALGLKSRMVGYSGISAWKTPNPELMAQLADLPELAARHPSLENLLNAEA
                     DFFFAGWNYGMRVGGEVTPASLARFGIPVYELSESCAHVMPRRVASLEDVYNDLHNLG
                     RIFAVQPRAEALVQGMQQRVAAVRARLANSGPAPRVFLYDSGEESPFTAGRLAMPQAL
                     IEAAGGRNVMDELAASWVRVGWEAVISSDPEVILIVDYGERSAAQKRDFLLQHPALQG
                     LTAIREQRFVVLPYLAVTPSLENAAAIETLAAALHPQAFGQ"
     gene            28713..29732
                     /locus_tag="VFF22_16310"
     CDS             28713..29732
                     /locus_tag="VFF22_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZX18414.1"
                     /translation="MIRSPRAYRLLLLVLALALALSCAASLGFGAAPVPLPRVLEILG
                     QRLLDLQPAQAVSSAQDSIVWLIRAPRVVLGALVGAGLALVGTALQAVTRNPLADPHL
                     LGVSSGAALGAVIVVLYLGEFIGLLSLPLAAFVGALVSMLLVLAIASRGGRLESDRLL
                     LAGVAVSFVMMAASNLLLFLGDHHAASSVIFWMLGGLGLARWELLWLPALCLVLALLL
                     LLGLARALNALMAGEHSAVSLGLEPRRVRLLVFVCASLLTGVLVSLSGAIGFVGLILP
                     HVARFLVGAEHRRLLPVSALLGALFLVWVDVAARTLLAPQDLPIGIATAAIGGVFFVL
                     LLRRR"
     gene            29850..30107
                     /locus_tag="VFF22_16315"
     CDS             29850..30107
                     /locus_tag="VFF22_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18415.1"
                     /translation="MTMKSTAATGAAIAFAAASLFAGLSSTVAVAEEAKVHCYGVTSC
                     KGQNDCKTAENSCKGQGACKGHGFKSMTLAECNAAKGTVGE"
     gene            30164..31009
                     /locus_tag="VFF22_16320"
     CDS             30164..31009
                     /locus_tag="VFF22_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HZX18416.1"
                     /translation="MNAQRGNLGFGLGLRSTYYQAILEQQPAIDWFEIVSENYLVAGG
                     KALYFLDAIGEQYPLVMHGVSLSIGGPHELDRDYLRQLKHLAARVQPAWISDHLCWSR
                     GNAHQLHDLLPLPYTEESLQHVAERVRQVQDVIERPLVLENVSSYLRCADDQFSEWQF
                     LAALSELSGCELLLDVNNVYVSARNHGFDAWTFISSLPKQRIRQLHLAGHSDYGRYLI
                     DTHDQPVSDPVWQLYQRTLQHLGPVSTLLERDDHFPDLGVLLDELNTARRFAAQALQG
                     TAQCA"
     gene            31000..31794
                     /locus_tag="VFF22_16325"
     CDS             31000..31794
                     /locus_tag="VFF22_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016984033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HZX18417.1"
                     /translation="MRLSDWQAQVQTYLQGPDAQPNPALQASLLGSAALSAEQGLAIY
                     HNAYRARLLEALRGDYPAVHGWLGDEEFDALALAYLDAHPSQHFSLRWLGAQLADFID
                     GYLIPAQAAPLSELARLEWAFTLAFDAPEGQPLSLTQMAELPAEDWPTLQVRLLPSVQ
                     WLACRHNSLAIWRASKEQGDFPGSQPLVATETCVVWRDQLITRYRSLAADEAAALQGM
                     TVQGWTFAELCGELAHLGEQAPAQAVSWLRQWLADGLLQRADQAQP"
     gene            31902..32489
                     /locus_tag="VFF22_16330"
     CDS             31902..32489
                     /locus_tag="VFF22_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HZX18418.1"
                     /translation="MNTLFYSMAGFALAASISPGPVNLLALSAGATHGLRRSMRHVTG
                     ATFGFTALLLAMGLGLQQLLQQWPALTHGVAWFGVAFLLYMAYGLARSDGQLSGNAAQ
                     QRPSLFKGALMQWLNPKAWLASMAGMGAYAADGDLWRVVQFAALYFVICYLSISCWAL
                     AGVYLRQYLENPLYLRRFNRGLALLLVLSAVYLLV"
     gene            complement(32490..33317)
                     /locus_tag="VFF22_16335"
     CDS             complement(32490..33317)
                     /locus_tag="VFF22_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZX18419.1"
                     /translation="MSTEQQATTPRFWRDPALPFVETREALDGRLLCYAKHSHECFSI
                     GTITQGRSTYLNEKAREQVAAGTLVLMNPGDVHACNPVDGQPWSYRMFYIDSTWLGDL
                     QHQLGFSHNPHLQPFSAILSRDPELFNGLNQLYDLLNAASVDQLRKECALIDYFSTLQ
                     QRLAPAPAPIREDNPRIRLAAEFIRAHCTQTLKLEDICSAAELSPSYLIRSFRQHYGM
                     TPHAYLLNCRVQVAQAGLKRGESIAEVALDAGFADQAHLQRTFKQLLAATPGQYRRA"
     gene            33460..35214
                     /locus_tag="VFF22_16340"
     CDS             33460..35214
                     /locus_tag="VFF22_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX18420.1"
                     /translation="MSAFLLRYRVVLIVLLILLGLLASIWLAGRYAEQRAWQERSLEA
                     RGQLQLYAQSIHTLVERFRSVPEVLALDSDIKSLLRAPDDSLLRSTLNERLEQLNTAA
                     GSNVLYLLNAKGETLVASNWRDWSSFVGNNYAFRPYFQDAVRQSSGRYFAVGVTTGIP
                     GYFLSHAVRDDNGVVLGVLVVKLELEELQREWASQPGVLLVADSYSVVILSNRPAWRF
                     LALDSLSEQARAELVEVRKYAEQALQPLASTLRRQIDSDSQWRRVEGPDGPRDYLWQR
                     QALGDEGWTLHLLHEPKTLVDSVRSYRLAAAGVWMTLAFLLLYLAQRRKTQRLQAGIR
                     ERLEREVALRTAELREAQEGLVHAAKMAALGQMSAAMAHEINQPLTAMQMQLGSLRLL
                     LDSGRQDDVREGLQRIDGLLQRIAGLTGHLKTFARKSPAGLSERLRLSDVLEQALQLL
                     APRLRSEQVQLHSEIDTEARVLGDAIRLEQVLLNLLNNALDAMADSTVLQLQICIERQ
                     AEQCLLSIADSGGGIAAEALDHVFEPFFTTKPVGAGLGLGLAVSYGIVRELGGSLEAA
                     NGEQGAVFTLRLPAAPNA"
     gene            35275..36627
                     /locus_tag="VFF22_16345"
     CDS             35275..36627
                     /locus_tag="VFF22_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZX18421.1"
                     /translation="MTGHVIVVDDEAAIREAVQQWLELSGFRVHSCATAQAALALVDR
                     DYPGIIISDVRMPGTDGMQLLDSVLERDRDLPVILITGHGDVPMAVQALRQGAYDFIE
                     KPFTPERLLDSVRRALDKRRLVCENRQLRQQFALKDHIEAQLLGVSRPMETLRRQILE
                     LAGTSVNILLRGDTGSGKERVARCLHDFSNRAGKPFVALNCAAIPEHLFESELFGHES
                     GAFTGAQGKRIGRIEHADGGTLFLDEVESLPLAQQVKLLRVLQEKTLERLGSNRSIQV
                     DLRVISAVKPDLLDEVKAGRFREDLYYRLNVATLRIPPLRERREDIPLLFEHFANQAA
                     QRHGRETPSLPPSELTRLLGHDWPGNVRELINAAERHALGLSGEVERGEGLPVQSLAE
                     QMEAFEAQCLHRALQHCQGNIAEVIALLQLPRRTLNEKMQRHGLQRSSYRPAGSSDED
                     "
     gene            37106..38059
                     /locus_tag="VFF22_16350"
     CDS             37106..38059
                     /locus_tag="VFF22_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZX18422.1"
                     /translation="MRMTKRFSLFAAAAAFTASTAVLAAPTFINILTGGTSGVYYPIG
                     VGLSQLYSNGIEGSKTSVQATKASVENLNLLQAGRGELALALGDSVADAWNGVEDAGF
                     KAPLKKLRAIAGTYPNYIQIVASKESGITTLADLKGKRISVGAPKSGTELNARAIFKA
                     AGLSYEDMGKVEFLPYAESVELIKNRQLDATLQSSGLGMAAIRDLAATLPITFVAIPA
                     EITAKIDNAAYQAAAIPAGTYDGQDSDVPTVAIQNILVSHDGVSEEVAYQMTKLMFDN
                     LERLGNAHSAAKDIKLEGAAKGLPIPLHPGAERFYKEAGAL"
     gene            38294..40321
                     /locus_tag="VFF22_16355"
     CDS             38294..40321
                     /locus_tag="VFF22_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HZX18423.1"
                     /translation="MSEQNQGLAANPSDWPKALFYVALLFSIFQIITAAFHPVSSQIL
                     RAVHVGFLLLVVFLCFPARGKEQPWQPMAWLLGLAGMATAFYQWYFEADLIQRSGDLT
                     QSDMVVGLVLIVLVFEAARLVMGIALPFICALFLAYGLFGQYLPGDLMHRGYAIDQIV
                     NQLAFGTEGLYGTPTYVSATYIFLFILFGAFLEQAGMIKLFTDFAMGLFGHKLGGPAK
                     VSVVSSALMGTITGSGVANVVTTGQFTIPLMKRFGYKPAFAGGVEATSSMGSQIMPPI
                     MGAVAFIMAETINVPFIEVAKAALIPALLYFGSVFWMVHLEAKRANLQGLPKDQCPNP
                     WAAIKERWYLLIPLFILIYLLFSGRTPLFSGMVGLALTAIVILGSAIILRVSSKVMRF
                     AFWIALGVLCAGFFQLGIGVVFGVVALLVAICWFIKGGRDTLVICLHALVEGARHAVP
                     VGIACALVGVIIGVVSLTGVASTFAGYILAIGQDNLFLSLILTMLTCLVLGMGIPTIP
                     NYIITSSIAAPALLELGVPLIVSHMFVFYFGIMADLTPPVALACFAAAPIAKESGLKI
                     SLWAIRIAVAGFVVPFMAVYEPALMLQGDSLLATLYVVLKALLAIGIWGAVFTGYLQA
                     KLSWWERALGFAAGASLILATPMSDEIGFALAALFIAQHVWRAHRAQVAAA"
     gene            40318..>40695
                     /locus_tag="VFF22_16360"
     CDS             40318..>40695
                     /locus_tag="VFF22_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HZX18424.1"
                     /translation="MIGLCLGLAGAVWAEVPTPSFTLAWTHTIEKIRWEEDYRVTPEG
                     LLLGEARVKGSGAGMEIPDGAELREGSWHYQRQLSPLQPLRLGRTPEAGDYQLCFNQR
                     CHSMSEWLGPPQATQPALELWSCE"
BASE COUNT         7040 a        12557 c        13296 g         7802 t
ORIGIN      
        1 catgggcctg cagaacgcgc tgattaccaa actctccagg gcggaaatcc gcaccaccca
       61 tatcaccgga atcatcaccg acatcggtat cgagctaggc aagctgttct attggaaccg
      121 cacgcgcggc cctgagatcg ctcgggtgat ggccgaccgc caacgcctac tggtgctcag
      181 cctgttggcg ctgtacttct tccttggtgg ggtgatcggc gcactgggtt tcaaccacct
      241 gggttatatc gccaccgtgc cgctggcttt gctgctggtg ttattggcca gtgtgccggc
      301 gctggatgat ctgttgctgc tcacgcggcg cgtattgcgc cgataagctt ggccgactgc
      361 ctgcggctca gtcctctgcc gacctgaaca gatgccgatg ctcggcgttg tacagcgccg
      421 agtcggcgaa ctgttcggcg tgcagcacgc ggccgacgag gatcagcgcg gtgcggcgaa
      481 agcccttggc gcgcacctgt tcctcgatgg tggccagggt gccgctgacc caatcctgat
      541 ccggccagct ggcgcgatgg accacggcaa tcgggcaatc cgcgccgtaa tgcggcagca
      601 gctcagtgac tatcttgctc agatgcacca cgcccaggtg gatggccatg gtcgcgccat
      661 ggcgcgccag atcgcccagt gcctcgcccg ctggcatgtt cgacttgctc gcgtagcgcg
      721 tgagaatcag cgtctgcgac acctcgggca gggtcagttc gctttccagc agggcagcgc
      781 acgccgcagt ggcagtcacc ccagggatga tctcgaaggc aatgcccagc cggcgcaggt
      841 gacggatctg ctcgccaatc gcgccataca gcgacgggtc tccggagtgc acgcgtgcca
      901 catcctggcc tttgtcgtgg gcctggcgaa tcagctcgat gatttcatcc aggtgcagct
      961 cggcggtgtt gatcacctgc tcggcgctgt ggccttcgag caccgctgcc ggtaccagcg
     1021 agccggcata gagaatcacc gggcagctgc acagcaggcg ctggcctttg agggtgatca
     1081 gctcggggtc gccggggccg gcgccgataa agtaaacggt catgacacat ccttgggtgc
     1141 aatcgacgcc agggcgcagg tggcgctggc gttacgggtt ttctcgccga gcaggcgggc
     1201 agcattgccg ctcaggcgtt cagccagggc cagggcgcag ggttcggcga cggccgggct
     1261 gccagtggcg gccagggtga gcggcgagcc ttgaatatcg tgctgataag gctgcagttc
     1321 ggctgcgcta aagcagctca gctgtagacc aaggcgttcg gccaatgcca gcaggcccgg
     1381 ctcgtcgcgc ttgtgcgcga tgctggccaa tcccgccagg ttatccacag ccagatccag
     1441 cgcctgcagg ctgttcagca gcaggctgaa taaatcctcc acgttgcagc cactgcggca
     1501 gcccaacccg gcgactacct gaatggcggc agcggggcgg cgagaggcgt tgctggggat
     1561 catctgcggc gctcggtgtg tgcggcggcg tagctcaaca tggcgaccgg cagcggtcaa
     1621 ccgccatgcg ctgtacctgg agagccgctt gtcttaggcc gctgagttta aggcgcttgc
     1681 agcttgttag cagttgaaat gggaacgatt cgcgattaat gtgcgcgcgg ttgccatgct
     1741 tatctgtggc gtagttcacc cctggttggc ttgggccgcg cgatgtcgct acctgaaacg
     1801 gattcgatca tttctctcga cgtgctctac ggcacgcatc gcggctggct gcatggctgg
     1861 ctgcggcgtt cggttggttg ttcgcagcag gcggcggatc tggttcagga cacctttgtg
     1921 cgtttgctgg tgcgtggcca gccgatcaac aatcgcgcgc cgcgcgcctt gctgacgcgt
     1981 attgctcgcg cgctggtgat cgatcactgg cgccgtgatg cgctggagcg cgcctatctc
     2041 gaagcgctgg cgcaggtgcc cgagtcgagt tatccctcgc cggaagttcg ccatgaggcg
     2101 ctgcagtctc tagagcgcat cgcgcaaatg ctcgatggtc ttaaaccggc ggtacgccag
     2161 gcctttctgc tgtatcagct cggcgagatg agccatgcgc aagtggcgca gcagttgggg
     2221 gtttccagcc gcaccgttga gcgccatgtg gccagtgcct tgctgcactg ctatcgcctg
     2281 tgtttcgagg tcgagcagtg agcgcggccg agcgtttgcc tgagcccatc gtctgcacgg
     2341 ctatcgagtg gcagatgcgt ttgcgcgctc atgccgacag tgccgagctg cgccggcagt
     2401 tgcaggactg gctaaatcaa gacgagcttc atcaacaggc ctggcaacgt ttgcagcaga
     2461 tggccgggct gtttcaggtc agccatctgc cggctgccag agaaaccatc cctctgctgc
     2521 aacgcgctga agccgatctg agtcggcggc gcatgctcaa gctgctcggc ttcggtctga
     2581 cagcgggcag cgcgacgctc ctggccacca gcacgccgcc ggcctggcgt gccgactttg
     2641 ccacggccac cggcgagagc cgtcgcgtca ggctgggtga tggcactcag gtgcagctca
     2701 atacgggcag tgctctggat gtgcaagggc agcagctgct gctacgtgcg ggtgaagtgc
     2761 tggtggaggg cgcgcaatgg cagctgcagt gccgtttcgc cgaatgccgg gggcgccagg
     2821 cgcgggtgct ggtgcgcgaa cgcgacggct acagcgaggt tcgcgtcgag cgcggcgaag
     2881 tgctggttgc caccgaggcg ggacagcgct ggctgcaggc cggcgaaggg ctgggggttt
     2941 cggcgacgac tatgagcgag ctcggtaagg gcccgctcga tccttttgcc tgggcgcgtg
     3001 gcctgttgct ggtcaacgat atccgcctgg ccgactttct cgccgaggcc gggcgctacc
     3061 gccagggctg gctgggctat gacccggcgg tggccgatct gcggctttcc ggggtgtttc
     3121 gcctcgatca accggagctg atgctgcgca acattaccca tctgctgcca gtggcaatag
     3181 tcgagcgcac gcgttggtgg gtccgcatcg tgccggtcgc ataaataaac tgtcggactt
     3241 ctgcgactca tccggttaag ggagatcatt ccctcaatgg atacccttca tgcgttctgc
     3301 cagaccactg ccatttcaac ttcagccgcg tattttgtct gctgccgtct gcctgggcct
     3361 ggcgctattc gcagggacgg cgagcctgtc gacccacgct gctgagccgg ctgccagcgt
     3421 ggctgcgccg aatgttccgg ccgggccgct ggatcaaaca ctcagcctct tcgccgagca
     3481 tgcgggaatc atcctgtcgt actccccgga tatggtccgc ggcttgagca gccggggcgt
     3541 ggcagggggc ggctcactga aagaagggct ggcgcagctg ttaggcggca ccggcctggt
     3601 cgccgaaaag accgcatccg gttacgtgat ccgcctgatc aaggccgacg acagctatca
     3661 actggaaccc atgaccatca acgcgacggc gacgcagagt gccttcgccc cggtgccggg
     3721 ttatttcgcc agtaacgcca gcagtgcgac gaaaagcgac aagccgattc tggagactgc
     3781 ccagagcatc agcgtggtga ctgccgagca gatggctgac cgcaaggtaa atcgggtcga
     3841 agatgcggtc gcctatacgg cgggcgtgcg agtcgggggc tcgggcctgg atccgcgttt
     3901 cgacaccatc aacgttcgtg gtttcgagac aacgcagtcc gccgactttc tcgatggtct
     3961 gcgccaggct ggcagtggct ggctagcctt gccttccatc gaggcctaca gcctggagcg
     4021 tatcgaagta ctcaaaggcc cggcttccgt gctctacggg caaattagcc ccggtggtat
     4081 ggtcaatcgg gtgagcaagc ggccaagcct gctggcgaaa aaccaggtcg aggtgcaggc
     4141 cggcaactac aaccaccgcc agggccagtt cgacatcggc ggcaagctgg atgaggcggg
     4201 tgatgtactg gtccgcaccg tgggcatcta tcgcgatgcc gagtactcca ttgagcagat
     4261 ggacaacaac acgcggctgc tggcgccttc gttgagctgg cagatcgacc cggataccag
     4321 cctgacgctg ctggcccagt atcaggaacg gcagacggct gcgtcgccga tgctctatcg
     4381 ggatggtgat cacctcacca acacctggca gggcgacgag tacttcgaca agctcgagca
     4441 gcgcaagtgg acggtaggct acgagttcga gcacgccttc aacgaaacct tcagcctgca
     4501 gcagaacctg cgttacggtc agttggacac caccaatcaa tacctggatg ttggggtggc
     4561 gagcgtcgac aaaattcttc ctcggagcac cgcaggtagc tatgaggata tgtccacgct
     4621 gtccaccgat acccgtctga tcagtcgctt cgccactggc aatctgcagc acacgctggt
     4681 cagcggcgtg gactatgcct ggctcgatac tgaactggtg tatgcctctg gtactgcacc
     4741 gtcactcgat ctcaatgcac cggattacca tcagccgatc tccgatcccg acaacgttct
     4801 ggtcgaccgc gaaggcctgg agcaccgcgc cggcatgtac ctgcaagatc aaatcgaaat
     4861 cgaccgttgg cgcctgtccg ccggcctgcg tcgcgactgg gtgcatgcgc gtaccaatgg
     4921 ctatgtgtgg ggcgaggacg tcaacaccaa gatcagcaac tccgccacca ctggctcggt
     4981 cggcgcgctc tatctgtttg acaatggtct ggcaccctac gccagctatg cgacctcgtt
     5041 cctgccgcag agcggcgctg agtttggtgg caagctgttt gaaccgagcg aaggcgagca
     5101 atacgaagtc ggcctcaagt atcagccgct gggcagtagc acgatgctca cggcttcgct
     5161 ctaccatctg gcccagagca atgtgtcgac ccgtgatccg aacaatgcca gcttccaggt
     5221 gcagacgggt gagcaggttt cccgcggcct ggagctggaa gctgtgtccg acctgaccga
     5281 cagtttgcgc atgagcgcca gctacagctt caacgacgcc gaagtgacca aggacagcga
     5341 cttcaaaggc aaggcaccga agaacacgcc acgtcacctc acttcgctct ggctgaacta
     5401 cagcttgccg ttcggcctcg gtgtatccgc tggtgcgcgc tacaccggca gcacctatgg
     5461 caacggcgag aacaccgcga agaacgagga ctacaccctg ttcgatgccg gcgtgcacta
     5521 cgacttcggc tccagtctgg acggcgtgcg ccttgccctg aatgcccgca acctgactga
     5581 caagcgctac atcaactgtc aggacggcta ctgctatcgc ggcgaggcgc gcagtgtggt
     5641 caccagcctg agttacacct ggtgagcgcc gtgaactggc tgcgcagcgc ggcgaccgca
     5701 cttgtgctgc tgggtggcgt gaatgtcgcc caggccgaac cggtggccct cgatggcacc
     5761 gagcaatggg cgatgcagag tgccgagggc cgcaattacc gcatcatgat cagcctgccg
     5821 gagggcgatg tgccctatac cggcggctat ccggtgatct atatggtgga tggcaatgcg
     5881 ctcttcccgg ccttccacgc tgccaagcgc gcgcaggagc gctggaaggg ctcgatcctg
     5941 gtggccatcg gctaccccag cgatacgccc tatgacttca accgccgcgc ctttgacctc
     6001 tcgccgccgc agttgcccga gcgcaacaaa ccgccgcagg gtggccagga cctgtttctc
     6061 gactttatcg agcaacgcct gatgcccaag gttaacgcgc gcttcaaggt cgatcaggac
     6121 cagcgcagcc tggtcggctg gtccttcggc ggtatgttcg gcatctacac gctgttcacc
     6181 cgcccggcgc tgtttcagca tgtggtcgcc atcagcccga gcctgtggtg gcgcgaccgc
     6241 tacctgctgg cacacgagcg cgcgttcacc gagcaggcgc atgccggcaa gctcgatctg
     6301 acgcatagca gcctgaccat gttgaccggt gaccgcgaaa tgccccagga aattcaggat
     6361 gtgcgctccc tgcaactgcg tatggaaaac ctctcgcagt acggcttgcg cagcgacttt
     6421 cagatcgaag cgggcgagga ccatatgtcg atccccttcc gcgccaccac gcgggtactg
     6481 gaggagttgc tcactaccag gcgttactga gcaacaagcg ctgatatcaa tggcggcggt
     6541 caaccgcgat tgaccggccg tcatggcctg cgtagccttg tcgcgttcga ggttcttcgt
     6601 gggcatgtcc atgaagctaa gagggaacaa ggtcgatacc ttggctgccc ccgcaactgt
     6661 gaacggtcta aagcccttcg atacaccact gcctcaggcg ggaaggtgaa gggtcgcgca
     6721 acgcgcagac cgtgagccag gagacctgcc tcgtcacgct ttcgacaacc gggcggggtg
     6781 atccggtggt gttggctgac gccccgtggc tgtcatgtct gtgccgcctc ctgctcgtct
     6841 ttctatcgac gtcatgcagg actcgccatg caaaccctcg ccaaactccc cgtcaccatc
     6901 gttaccggct ttctcggcgc cggcaaaacc accctgctcc gccatatgct cggccacgcc
     6961 gacgggcgcc gcattgcggt gatcgtcaac gaattcggcg agctgggcat cgacggcgaa
     7021 atcctcaagc agtgctccat cggttgcagc gaagaagaag ccaacggccg catcttcgaa
     7081 ctggccaacg gctgcctgtg ctgcaccgtg caggaagaat tctttccggt gatgcgcgag
     7141 ctggtggcgc gccgtggcga gctcgaccac atcctgattg aaacctccgg ccttgccttg
     7201 cccaagccgc tggtgcaggc ctttaactgg ccggaaatcc gcaacgcctg caccgtcgat
     7261 gcggtaatca ccgtggtcga cagcccggcc gtggccgccg gcaccttcgc cgccttcccc
     7321 gatcaggtcg atgcgcagcg caagctcgac cccaatctcg accacgagtc gccgctgcac
     7381 gagctgttcg cagaccagct ggccagcgcc gacctggtga tcctcaacaa ggccgacctg
     7441 ctcgacgccg ccgctctggc tgcggtgcgt gccgaagtgg ccgaagagct gccgccggcg
     7501 gtgaaaatca tcgaagccca tggcggcgag ctgccgctgg acgtgctgct gggcctgaac
     7561 tgcgagaccg agctgcatat cgagggccgc aagacccacc atgatctgga aggccatgaa
     7621 gaccacgatc acgacgaatt cgcctcgttc cacgtggaac tgcctgaagc cgaggaggcc
     7681 cgtttgctgc aagcgctgaa ggaggcggtc agcaaacacg gcatcctgcg catcaagggt
     7741 tttgcggcga ttcccggcaa gccgatgcgc ctgttgctgc aaggcgttgg tcagcgtttc
     7801 gataaacact tcgaccgcgc ctggcaggcc gatgaggcgc gtatcactcg cctggtggtg
     7861 attggccaga ccctggatca ggccgctatc gaagccgagc taaacgccgc gctggcaggc
     7921 taagcccgca tgcacctgct gcgcacccag cccggcagcc agttgccggt cgacagcatt
     7981 gccgacctcg gccaaacccc ggccgagctg gtgattctct gtaccggcga ttcgcacctg
     8041 tcgttgctgg ccgaggtggc gcggcagttg ccggcggatt accccagcct gcgcctggcc
     8101 agcccggcgc agctgagtaa tcacgcctcg gtggatctgt acgtcgagca ggtgttgcag
     8161 cacgccaagg tcatcctgat ttccgtgcac ggcggcgtca gctattggcg ttacggcatt
     8221 gagcgtctgg tcgagctggc cgagcgcggc gcgcgggtga ttatggtgcc gggttgcgat
     8281 aaccccgatc cggagctgat ggccctgagc aacgtgcctg tggccgaggc cgagcggctc
     8341 tggcagttcc tgcgacaggg cggcgcgggc aatgcgttgg agctattcaa ctgcatcgcc
     8401 agccagtggc tacagcgcga gtattcttgg gtagaaccac agccgctgcc acgcgtcggc
     8461 ctgtatcacc cgcacttggc caacccgagc ctggccgatt ggcaagccaa ctggcaggtt
     8521 gatgcgccgg tggcggcgct gttgttctac cgcacccagg tgcaggcggc gaataccggg
     8581 tttattgatg tgttctgcca gcgcttgcag gcccagggcc ttaatccgct gccgattgcc
     8641 gtggccagcc ttaaggaagc cgcctgcctg gatcaggtcg aggactggct ggagcagacc
     8701 gaggcggcgc tgattatcaa cacaacggcc tttgccttgt ctaacccgga agcgccgagc
     8761 gcacgaccct ttcgtcgtga tattccggtg ctgcaagcca tttgcgcgct ggataaccat
     8821 gagcagtggc aagccaatgc ccaggggctg ggttcgcgcg acctggccat gcatatcgtg
     8881 ctgcctgagc tggatggccg gcttatcacc cagccgatca gtttcaaggg cctggcctgg
     8941 cgcagcgagc gcagccagag cgatgtggtc tgttaccagc cgcatctgcc ggggatggat
     9001 ttcgtcgccg agctggcgcg caactggatc gccctggcgc acaaaagcaa cgccgacaag
     9061 cgcatcgccc tgatcctcgc caactacccg acccgcgacg ggcgcatcgg caatggcgtc
     9121 ggtctggata ccccggcggc ggcgctaaat atcctcaagg cgctgcagca gcagggctat
     9181 ccggtggaag agttgcccga cagcggcacc gcgttgatcc accagctgct tggcggcgtg
     9241 accaatgatc tggacatgct cgacgcgcgg ccctgcgcgc agagcctggc gctggatgag
     9301 tacctggcgt tcttccacag cctgcccgca gctaaccagc aggcggtgcg cgagcgttgg
     9361 ggcgagccgc agcaggactc tatgttccgc agcggtcggc tgatgatcgc cggcctgcgt
     9421 tttggtttga cctttgtcgg catccagccg gcgcgcggtt atcagctgga cgcggcagcg
     9481 gtgtatcacg accccgatct ggtgccgccg cacggctacc tggcgttcta ctgctggctg
     9541 cgcaaggcct ttgcttgtga cgcggtgatc catgtcggca agcacggcaa tctggaatgg
     9601 ctgccgggca agagcgtcgg cctgtccgaa caatgctggc cgagcgcgat tctcggcccg
     9661 ctgccgaata tctacccctt tatcgtcaac gatccgggcg agggcgcgca ggccaaacgg
     9721 cgtacccagg cggtgattat cgaccacctg atgccgccac tgacccgcgc cgaaagctac
     9781 ggcccgctgc gcgatctgga gcgcttggct gacgaatatt acgaagccag ccagctcgat
     9841 ctgcgccgcg ccgccgagtt gcgtggcgag attttgctca aggtgcgcga ggccagcctc
     9901 gaccgcgagc tgggcctgca actgaatgca gaccccaata gctggctgcc gcagctggat
     9961 gcttacctgt gcgacctcaa ggaatcgcag atccgcgatg gtttgcatgt attcggtgaa
    10021 tcgcccgccg gcaccctgcg ccgcgacacc ttgctggcgc tgctgcgcat tccccggggc
    10081 gacggccagg gcggtaatgc cagcctgctg cgcgccttgg cccgcgacct tgagctggac
    10141 ttcgatccgc tcgactgcga tatggccgcg ccctggttag gcctgcgccc ggtcgtgttg
    10201 cagagcctga gcgatgacag ctggcgcagc gctggcgata cccgcgagcg tctggaatta
    10261 ctggccttgc agctgatcgg cgcgacggat tttgaatccg ttggcattga aagcacccag
    10321 gtgctgcaac gcctgcgcga gcagatcgcg ccgttgctgg atgcctgcgg cccggccgag
    10381 atgcacggat tgctggcagc gctaagcgga cgcttcgtgc ctgccgggcc gagtggcgca
    10441 ccgagtcgcg ggcggctgga tgtactgccc accgggcgca atttctacag cgtcgatgtg
    10501 cgcaatctgc cgaccccgac cgcctggcgt atcggtgtgc aggcggcgga tcggctgctg
    10561 gagagacatc agcaggacca cggtgatcac ctgcgtcagc ttggtctgtc gatgtggggc
    10621 accgcgacca tgcgcactgg cggcgacgat atggcccagg ccctcgcgct gatgggcgtg
    10681 cgcccggtgt ggcaggccgg tagccagcgc gtcgaacgct tcgaggtgct gccgctggcg
    10741 cagctcggcc gcccgcgggt ggacgtgacc ctgcgcgtgt ccggcttttt ccgcgatgcc
    10801 tttagcaacc tgatccgcct gttcgatgac gcggtacagg cggtggtcga tctcgatgag
    10861 ccggaagaga tgaacccgct gtcggcgcgg gtctggcgcg aatccctgac cttgcaggac
    10921 agcgggctgg atgaacaaga ggcacgcaaa caggccggct ggcgggtgtt cggctccaag
    10981 ccgggggcct atggcgcggg cgtgcagaac gccatcgaag aacgtctgtg gcaaacccgt
    11041 gaggatctgg ccgaggtcta cctgaactgg ggcggctacg cctacggcac ccatagcgaa
    11101 ggcaccccgg cgcgcgcgca gttcgccgag cgcctggagc agatgcaggc ggtgctgcac
    11161 aaccaggaca accgcgagca cgacatcctc gactccaacg actactacca gttccagggc
    11221 ggcatgctcg cggcggtgga aaccctgcgc ggcgccaagg tggccagcta ccacggcgac
    11281 aacagccagc ccgacacgcc gcgtatccgc accctgaagg aagaactgaa tcgcgtggtg
    11341 cgcgcccgcg cggccaatcc caagtggatc gacggcatga agcgccacgg ctacaagggc
    11401 gcgttcgagc tggcggcgac catcgactac ctgttcgcct tcgacgccac cagcgaactg
    11461 gtcgacgacc accagtacgc gctgcttacc gatgcctatt tgctcgacaa aagcacccgt
    11521 gactttatcc agcagcacaa ccccggtgcc ctgcaggata tcctcgaacg cctgctcgaa
    11581 gcccagcaac gcggcctctg gcagcacccc ggcgagtacc gcgaggcgtt ggaaaatctg
    11641 ctgattgata gcgaggaagc atgaacgtgc cggtgcgatc atcgaccacc tacgctgctg
    11701 cgtctgacac tccggttgtg ggaggcgctt tagcggcgaa aagctcgcgg ctaaagcccc
    11761 tcctacagat gatgagcgct tctgtagggt gggttagccg catgtcgcgg ttgttgaaca
    11821 acacaccgaa attgatgcgg cgtaacccac catgggcgca acgcatacca ccctacgttt
    11881 gctcgaacgg attacgccca tgaccgaaca ccacttcccc ctcgccgccg tggtcgccgc
    11941 cgatgacctg aaactggcgc tgtgcctggc ggccatcgac ccggccatcg gcggcgtgct
    12001 gatcgaaggg ccgcgcggta tggccaaatc gactctggcc cgcggtctgg ctgatctgct
    12061 gaccagcggc acctttgtca ccctgccgct gggcgcgagc gaggagcgca tcgttggcac
    12121 cctcgatctg gacaccgccc tgggtgaggg ccgcacgcag ttcagccccg gtctgctggc
    12181 caaggccaat ggcggcgtgc tgtatgtcga tgaggtcaat ctgctgcccg atcatctggt
    12241 cgacctgctg ctggatgccg ccgcgagcgg ggtcaaccat atcgagcgcg acggcatctc
    12301 ccatcggcat gccgcgcgtt ttgtgctgat cggcaccatg aacggcgagg agggcgagct
    12361 acggccgcaa ctgctggatc gcttcggcct taacctggcc ctgaacgccc agccgcaacc
    12421 ggcccagcgc gccgagatcg ttcgccgccg cctggccttc gatgccgatc cgcaggcctt
    12481 tctgcagcgc tggcaagcgc agcaggacgc gctgcgcgaa cgttgccaga acgcccgtgc
    12541 gcgactggcg gctattccgc tggatgacgc cgccctggag cagatcagcc agcgttgttt
    12601 cgccgccgcc gtcgatggtc tgcgtgccga tctggtctgg ctgcgcgccg ctcgcgcgca
    12661 tgcagcctgg cggggtgccg aacacattga atctgtggat atcgatgcgg tggcagactt
    12721 cgtcctgcgt caccgtcgcc gacataactc gccgcctgcc gcacaaccgc cacaacccca
    12781 gtcgcaatct cagcccagca acgataagcc cagcgagcag ccgcaaggcg aaggccagtg
    12841 gggcgaatta cccgcccagg cgcagagcct cggcgttcgg cgtgaaccgc cgcgctgggc
    12901 aaaaaagccc taggcatccg cccgcgcaca gccccaggcg cggatgcccg ggccgctccc
    12961 ggcacactgg gcggtggtcg taatggcgcc agtcgcagtg gcgcggcggg ccgtatcgac
    13021 tggccagcga gcctgcttaa aggccgcccg cgttgccgtc aggatcttgt tttgcgtcca
    13081 cgcagcgcca agcccactga actctggctg gtgatcgtcg acgcctccgc ctcgactcgt
    13141 cgccatggcg cgctaagcaa ggccaaaggc ctgctcagtg aggtgttcga gcaggcgcgc
    13201 cggcaacgcg cgcgcctggc tttattgcac gccaccggcc gccaggcgca atggttgtgg
    13261 caggggcaaa aagcctccca tgccttgcag cagtggttgg ccgaattggg cgcaggcggc
    13321 ggcacgccgt tgctggcggc cgtgcagcag gccgccgact ggcaggcgcg gcgtcagcgc
    13381 ctgcacccgg gcgagcatca gcgcttgttg atcattaccg atggccgcct gcgcgattgg
    13441 ccgacgttaa ccccgagcgt ctgcccggcg ctgctggtgg atatcgaaag cgcaccgatc
    13501 cgcctgggcc gtgggcgtca actggctgcc gagttgggcg cggactaccg acatattgat
    13561 tcgctgccgc tgttagccgg caacctggag accgccttat gaaaaacctg ttgattatcg
    13621 gcatcggtgc cggcgacccg gactacctga ccgtgcaggc gatcaacgcg ctgaaccgca
    13681 cggatgtgtt ctttctgatg gacaagggca gcaccaagga cagcctgatc ggcctgcgca
    13741 agaccatctg cgagcgcttt atccagggcc gcgactaccg ctttgccagc gccgactgcc
    13801 cggagcgggt acgcgatgtg ccggactatc gcggcagcgt ggtcgacctg aatcaggaca
    13861 agcagcaggt gttcgagggc atgatccgcg agcagatggc cgacggcgaa accggcgctt
    13921 ttctggtctg gggcgacccg gcgctgtacg acagcaccct gcgcatcgtc gagcaaatcg
    13981 tggccaacag cagcgaacag attgcgtacg aagtgatccc cggcatcacc agcctgcaag
    14041 ccctggcggc caagcataag gtctgcttta acagcatcgg ccgggccttc cagatcaccc
    14101 cggcgcgccg cctggccgaa gccggctttc ccgagggctt ggacagcgtg ctggtgatgc
    14161 tcgacgccca agacacctac cagcgctttg tcgaccagcc catgcagatc tactggggcg
    14221 cctatattgg caccccggat gaagtgctga tcgctgggcc gctaagcgag gtcgccgaga
    14281 ccattcgcac ccgccgcgcc gaactgcgcg agcagcatgg ctggattatg gatagctatc
    14341 tgctgcgtag aagcggcacg ggctgaggcc gttacgaaaa acgcgacact ggaagccact
    14401 caacggaaag tgatggaaag cgtcgctttc tgtttagtca gcgctttgga aatggagata
    14461 acgaaggaca gaccctgagg caagaacagg gcagcgctgg tattgccgca tgcgacgccg
    14521 atggcggagt attcttggca gggcctcatt tatctaccgg gagcaacccc atgttttccc
    14581 atatctgcat cggtgtgagt agcttcccgc gagcgctggc cttctatcag ccgatcatgg
    14641 cgctgctggg tatcgagctg cgttttcttg atgagcacaa accctgggcg gcctggcaat
    14701 cgaagccgga accccggccg ctgctgctga tcggcaaacc ccatgatggt gccccgcatc
    14761 acccaggcaa cgggcagatg gtggcactgt tggcgcagaa ccgcgcccag gtcgagcagg
    14821 cccatcgact ggcgcttgcc cacggtgggc actgtgaagg gccaccgggg ctacgcgccg
    14881 agtatcaccc ggactactac ggtgcctact ttcgcgaccc cgacggcaac aagctctgtg
    14941 tggtctgcca ccagcccgag tgagtcgccc ctagcggggt caggccaagc agcctggagg
    15001 atgggttgag cgtagagata cccatgctga aatcaatgac actgacccca ttgatacgcc
    15061 agcgctttgt cgaccaggcc atgcatatct actggggtgc ctatatcggc accccagacg
    15121 aagtactgat cgctgggccg ctagcggaag tcgcagagac catccgcacc tgccgctttg
    15181 agctgcgtga gcagcatggg tggattatgg atagctattt gctgaggaaa gactgacagt
    15241 tctcacgctc tagcgcgggc aatgatcaat tgaggtgctg ggcaggcaag cgaaatattg
    15301 tccgtagtgg attatccagg ttacttgttc taataatctg tgaataaata ttgagcattt
    15361 tggggggcgc atggccgcaa agtggaaaaa gaaatctaaa cccgagttag tgattgatcg
    15421 aataaaaaag catgcatcaa ttaacgataa agggattttg tcatttgcgg ggtttgagtt
    15481 ttttcaatat gaagctgcgc tgcatgcaat aattgatttt ggagaaaata taagctttaa
    15541 aactaagaaa gagcttcgag ataaatcttt gagagcatgc ttgcgaaatg gatctttaac
    15601 cgctaaagac tttataaata caattaacaa agagatttct atatacaagc agaaacaaga
    15661 ttctgttttc gtgctaacca catctgtatc cctttcaggt gggctgccaa tttcaaagat
    15721 agttcttcca gacgctgttg ttcaaagcta ctccaaagga cttccaaaga agtacaaaaa
    15781 tagaagtgct catgataata aatggttgga gctattttct gactcgccat tgcccccttt
    15841 atatagtcat gtggctgtaa gagttaatgc gaaaaggcca tctgatgcat tcgatatagc
    15901 tgtcaatagc cttgatttta taagaggcgt tgtaggatta aatgtaaatt cgtcatatca
    15961 aatttcttta ggcactggtt tatcaaaacc tttaaataaa gtaatgcttg gtggaatgca
    16021 ttgcttgcat tttgaaaatg gggaactggt atttcctgat tttttttggt acgagcgaca
    16081 ttataaagaa cgtagtgtta cagatataaa cgaacggcga aaatatatag ccaaaggatg
    16141 caggttttta cttggtagtc tatcaaaata tccaaaggaa gattttgatt tgttgagaaa
    16201 tgctatcgca agatatgcaa gagcttttga tgaatacgat cgtgatgtgt tgattcaaaa
    16261 aatgtgggct gcacttgagt ctattgttgc atctggagaa aataatgcag atgtgattgt
    16321 gcgtcgctgc tcctatttgt actcggatag ggagtatgca tcccaagtct tagaatatat
    16381 tagagattat aggaaccaaa atgtacatcg cgggcattca gaagaaggtc ttgatcacta
    16441 ttgtttcgaa ttacagaaat ttttccgtga actagtgata ttttatgcgc gcttttccag
    16501 gttgtttacg tctttaagtg aggcaaaccg gtttttggat tgttcagata atgagagaaa
    16561 gttggaaaga gaaatcgaga ttttaactag agcaaaaaaa ttcagatttg ggtagttcgt
    16621 aggatgggtt gagcgcagcg atacccatgc tgctgcgaac ccttgatacc cccgatgggt
    16681 atcgcaggct caacccatcc tacgtccgtt acatctgtgg ataactccgc ggcgtataca
    16741 cccactcgcc accctcggca cgcggaaagc ggcaggtttg gctagagccg ataatcacca
    16801 gcgtgcgcat atcgaccatc tctggcgtca gttcaccgag ggtgagggta cgcagggttt
    16861 cgccggggcg gccgatatcg cggcctagta ccaccagggt ttccggggtg cgttgctggc
    16921 ggatgatttc cagggcagag ccgagctgcc aggggcgggc tttggagatg gggttgtaga
    16981 aggccatcac caggtcggcg gcggcggcgt gggccaggcg cttttcgata atcgtccagg
    17041 gcttgaggtt gtccgacagg gaaatcaggc agaagtcgtg gcccagcggc gcaccggcct
    17101 tggctgcggt ggccagcgca gcggaaacgc cggggaatac ctgtagatcg acccgctgcc
    17161 attctgcgtc ggccgattca tgcagggcct ccagcacggc ggcggccatg gcgaagacgc
    17221 cggggtcgcc ggacgacacc accaccacgc gccgaccgct ggcggccaac tcgaaggcat
    17281 ggcgggctcg ctgcatttcc tcgcggttat cggtgcaatg gcgcacctgc tctgggcgca
    17341 gtgggccggc catattgata taggtttcgt agccgagcag gtcctgcgct tcatccagcg
    17401 cttggcgcgc ggcgggcacc ataaattcgg cggcacctgg gccgaggccg atcaccgtca
    17461 ggcggccacg ggggcggccg agttgcgtgg cgtcgatagg cgcggcggcc agcagcagac
    17521 gcagattggc gtgctggcta tgcagcggcg gcagttggct ggcgtcgtcg ataaagcgca
    17581 gcggcaattt cagttgcgcg gcggtgtggt gcagttcggt gttggccatc agttccggcg
    17641 cggccagcag gcaggccagg ctctgcgggg ccagttcgct gctgtgtagc gcctgctgca
    17701 gttcgctaag taattccgca tcggcgcgtt cgacccatac ggccacctgt tgcggatgga
    17761 ttagcagctc atcggcattc gccgcgcggt tgtgcgggct gatatggatc acccggttag
    17821 cctggttatc caccggcagt ttggcctggg ccagccaggg cgcgtcgcct tcaatacgca
    17881 cggcttggcc accgagcaag tcgctgacaa aacctttgcc ctgttccaga tcggccagca
    17941 catagcctgc tggcggctcc agcaggcagg tgccgaaacg cagttcgccg ctggtggtga
    18001 tcgccgcgct gacctgcaga tgcgcagcaa tctcccgcgc catgcggttg accccgccga
    18061 ggccgccaag cagcggcacc acggcgctgc cgtcttcggc cacggccagc acgggcggct
    18121 cgctgtcttt ctcgccgagc acggcggcca ggctgcgaat cacaatgcca gcggcgcaca
    18181 gtacgatcag cggcgtaccg gcgcggtaca gcgtgcgcag ggtgtcgccg aagtcttcat
    18241 agatccgctc aacgtccgcc actcggccgc gcaggccgtg gatctcagcc tgtggataaa
    18301 gtgcctggat gcgccgcgcg caaggcaggg cggaggggcc gaggatgatg atggcggggg
    18361 cgctggtcat aaggtgttcc gtggttggcg taggagcggc cttggccgcg atcaatcgtt
    18421 taggcgtggc gattagcgct aaggctactt gcgtatcgcg ggcaagcccg ctcctacagt
    18481 gggtagggtg gatgacgatt ttctcatcca ccatcgcggt tggctgatgg gtgaagcgtc
    18541 atccgcctta cgggttcgtc gcggctaaag cggatcgccg cccggcccct cccacagtgt
    18601 taaccgcgcc atttctcgcc gggaattacg atcatcgaga agtacggcga agacatcggc
    18661 tccacctcat ccagcggcac gatgcgctgg ttacccatgg tcgcgcgttc gacgtaatgg
    18721 gcgcgctggt cgatgcccag ctcctgcagc acgcggcgga ctttctcgaa gttgcgcccg
    18781 agcttcatca ccacggcggc ttcggcgctg tgcaggcgtt gcttgagttc atcttcgggc
    18841 agcacgccgg agagcacgct caagctctgg ttgcgataca ccagcggggt gccgagcacc
    18901 gaggcgcagc cgagcatgga gcacacgccg ggcaccactt ccacgtcgta acggtcggcc
    18961 aggcggtcgt gcaggtacat ataggagccg tagaaaaacg ggtcgccttc gcagatcacc
    19021 gccacgtcgt ggccggcgtc gagccgggca gcgatctgca ccgcgcaggt gtcgtagaaa
    19081 tcgctgatca catcttcata gctcagcggc gcggcgagct tttccgtggt caccgggtac
    19141 accagcggca ggcgctgctg cgtgtccgtc aggtgctgtt cgataatgcc gaaggcgttg
    19201 ccgccctggc ctttgttggc tttggccttg gccacaaagt agccaaccac ggcggccgat
    19261 tgcagcaggc gcagggcctt gagggtgatc agctccgggt caccggggcc gacgccgatg
    19321 ccgagcaggc ggcctttttg cacactcatc actccacctc cgtggccaaa gcgttgaccg
    19381 ccgccaccgc catggcgctg ccgccacgac ggccgcgcac gatcacatag ggcacgccac
    19441 ggctgtcggc ggcgagggcg tccttggatt ccgccgcgcc gataaagccc accggcatgc
    19501 cgataatcag cgccggtttt ggcgcgccgg cgtcgagcat ttccagcagg tagaacagcg
    19561 cggtgggggc gttgccgatc accaccacgc tgccttccag gtgctcgcgc cagtgttcca
    19621 atgcgactgc cgaacgggta ttgcccagtg cgcgggcctg ctcaatcacg ccgtcgttat
    19681 gcagggtgca gatcaccggg ttattggcgg ccaggcgcgg gcgggtgatg ccttcggcga
    19741 ccatccgcgc atcgcagaga atcgccgcgc ccttggccaa cgcagcgcgg ccagcagcgc
    19801 cggcaccggg ggagaagcgc aggtcctgca ccacatcgac catgccgcag gcgtgaatga
    19861 cgcgcacggc gagcttttcc aggtctgccg ggatcgccga caggtcggcc tcggcgcgga
    19921 tggtggcgaa ggactgacgg tagatttcct ggccgtcgcg gatgtactca agcatcgctg
    19981 tttcctgttg cgcagtgggc ggcgaaccag tcgccggcct cgtcgatagt catggcgctg
    20041 gcctgcagct gaccgaaacc ggctgcctcg ggcgtgcgtt gataaagctg gtaatgcccg
    20101 gcactgctgg ccagcaaggt aaagggcgcg gtatgcgcgg cggcgcagga gcgcgggcag
    20161 gcgctgagat gcacctgcgg gcgcgccgtg ctggcgcgca ggcgttcggc caggcgcata
    20221 gcgtcagcct tgctatctgc caggcctttg ccgcaggcgg ctgagccggt gcaggcgatc
    20281 agattgctca agggttcctg tgcatcgatc agcaggccga gttcgcccag cgcgtgcagc
    20341 aggttgtctg ccgaatgctc ggggatattc ggcagcagca gaccctgcca gggcgtcagg
    20401 cgcaggctgg catcgccgta gtgctcggcc agaccggcca gggcgttgag ttgtacggcg
    20461 tcgatccggc ccagacgtga agcggcggca acgatgcaaa gggcgttttg ctgctgtgga
    20521 taaatgccca tcggcgcgcg cgggctgact ggtttgtgtt gccaatcggt cggtggcgcc
    20581 tgcacggtaa agggcaggcg cgtttgcagc tgttgcagca gttggtgggc gggaatcacg
    20641 ctcagcagct ggcgcatccg gctatgttcg ctgccagcca gatcgaggaa cagcctgagt
    20701 agctgttcca ccagcggcac ggcttgctca acgtcgacca cgcccagcgg cgtgtcagtc
    20761 gggcagccag ccaggccaaa cgtcagtctg gctggcgagc cgggcagggc gctgagccag
    20821 atatcgtggg ggtgttcgag catcgccagg gcttcgccgc cgtccagctg caaggcgaac
    20881 ttggccgaca gcccatgcag cgccggggtg gtttgcagca ggtcgagcag cgcggcggcc
    20941 agtgggcggg tgtcgagcaa ggcctgcggg tcgaggccgg cggcggggct gagcagcagg
    21001 ttgcgcacat cgtcggcggc cgggttgctc gggcccaggc cggcagccag caggcgctcg
    21061 atcagctcag cctgttggac gggcaatacg ccacgaatct gcagattgct gcggttggtc
    21121 agctccagca caccgctggc gcagcgctcg gccgcctcgg cgatggcgcg tgcctgttgg
    21181 ctgctgagta cgccgccggc cagcttgacc cggcagatgc cgccgtccag cgccggaacg
    21241 atacgcagca ggccggggca cgcgctaggg cgtgggtcgg gcaggtcggc ggaggttgga
    21301 cggtcgcgca aaaagggtca ccaacaacaa agacgcggcg accgaaccag cgaaacccgc
    21361 ccacagggga tttcatggca tcggtacacc ccgcccgatg ttggcactcg cgactagcgt
    21421 cgtcggcagg tctcctggct gacaggtcat catctggcgc ggccttccca gtttcccagt
    21481 ggcgcagtgg cggcagactc gctgtttaca gttgcggggg cagctcggtc acgatcagca
    21541 gatcgctccg gattccctct taggcccctc gcaccatggc ggtgggcacc gacgaaggct
    21601 gtattatgcc cgctttgttt gatgcgctga caccctagct tgtcgggcgg gggcatagga
    21661 gtgggtatga cagcctggtt aaccctggtg ggcattggtg aagacggtta cgccgggctg
    21721 ggtgaggcag cgcgccaggc gctgggcgag gcgcagtgga ttgtcggcag cccacgccag
    21781 ctggcgctgt tgcctagcga tctacccggg cagcacgaac gctggcccag cccctttagc
    21841 ctggagccgg taatggcgcg gcgcgggatg ccggtgtgcg tgctggccag cggcgacccg
    21901 ctgttctatg gggttggcgc gagcctggcg cgtcagctgc cggctgagga attgcgggtg
    21961 ttctctgggc cctcatccgt ctcactggct gctgctcgcc tgggttggcc actgcaggag
    22021 gtgacggtgc tgtcgcttgt ggcgcggccg ttggcggcgc ttcaggcgca gattttcccc
    22081 ggcgcgcggc tgctgctgtt gagtaatgac ggcgacagtc cggcggcggt ggccgagctg
    22141 ctgcgtgcgc ggggttttgg ccccagccgg ctgaccgtgc tggagcacct gggcggcgat
    22201 caggaacggc gcatcgatgg tctggccagc gactgggccg tgccgcacgt tgccgatctt
    22261 aatctgctgg caattgagtg cgtggccgct gccgatgcgc gtctgttgcc gctaaccccg
    22321 ggcttgccgg atgacgccta tcagcacgac ggccagctga ccaagcgcga tgtgcgcgct
    22381 atcaccctgg cgcgtctggc gccgcagccc ggtgagctgc tctgggatgt tggtgccggc
    22441 tgcggctcca tcggcatcga gtggatgcgc acgcacccca gctgccgggc gctggccatt
    22501 gagaccgatg ccggtcgtca ggcgcatatc cagcacaacc gcgatgccct tggcgtgccc
    22561 ggcctgcaac tggtcgccgg ccgcgcgccc gaggcactgg ccggattggc ggcgccggat
    22621 gcgattttta tcggcggtgg ggtgactgtt cttggtgtgc tggaacagtg ctgggccagc
    22681 ctgaaaccgg gcgggcgtct ggtggccaat gccgtgaccc tgcaaagcga ggcggcgctg
    22741 gtggcctggc gcgagcaggt gggtggcgag ctgacccgta tcagcgtggc ccaggcgcag
    22801 ccgctgggcg gtttcgacac atggcgcagc gccttgccga tcaccctgct ggaggtgcgc
    22861 aagccgtgag cccgcgcatt ctgctgttgg gcggcaccac cgaggcgctg cgtctggcgc
    22921 gtcgcctgag tactgaagcg atctacagcc tggccgggct gggccgggtg ccggatgact
    22981 tgccctgtca ggtgcgcgtc ggtggctttg gcggggccga aggtttggcg acgtttatcc
    23041 gcgaacaggg cgtcgaactg ctgctggatc tgacccaccc ctatgccgcg cagatcagcg
    23101 ccaacgcggc gcgcgcttgc gagttgagcg ggattgcctg ctgggcgctg cgccgccctg
    23161 gttggcaggc cggcgccggc gatgactggc gcgaagtgga caactggacc ggtctggttg
    23221 ccgcgctaaa cgacttccag cgtccgctgt ttaccctggg ccgcgagccg ctggaacacc
    23281 tgcacgagat acccctgcat cagcactgga ccgtccgttg tctgacggcc cagcaaggcg
    23341 tgccgcgtgc ggagattatc ggggcgcgcg ggccgttcgc gctggacgag gaacgcgcgt
    23401 tattcgctcg cctgggcacc gatgtgctga tcagcaagaa cagtggcagc cagtccaccg
    23461 agccgaaatt gcaggtggcg cgcgagttgg gtttgccggt attgctgctg cgccggccgc
    23521 tcttgccgag tgtggaccgc gagtttgtta gcctcgaagc gctgaccgag gcgctgcagt
    23581 tatagggcaa gttggccgcc gagcggttgc ccccaaggca aaaaatcccc tgttagactg
    23641 ccgccccgct ttggaggccg ccatgaccac gcccgtttcc ccttacgccc gcattctgtt
    23701 tgtcggcccc tacctgaccg agggctcgtt tgccgagcta tttcgtcgcc gtttgactga
    23761 gctgcgcggg gcagcggcgc tggccgatat cgtcgatacc agtgagggtt atgcgccgct
    23821 ctggcagcgc gtggccgaag gtgagcggcc gctgctggtg atcgacctcg agccgcagag
    23881 cagcagcccg cacctcgatt ggctgcgcag cgaactttgc cagcaggcca accctgagca
    23941 gctatttgtc gccagtgcga ttggccaggc cgagggcttg ccggtggagg ccgcctgcgc
    24001 gctgattgag cgtcccgagc tgcacctgcc ttgtgtcggt gtggctgcgg tgccggggca
    24061 tcacgcctgg tcgcagattc cgccgcatgc gcagcgcctg ctgttgtgca acggcccgcg
    24121 ctgcacccgg cgtggcgcgc tggacctgtg gaaaaccctg cgtcagcgcc tcaaggccgc
    24181 tggcaaactg gagtgtgaag gcggggtgca tatcacccgt acgcagtgcc agttcccctg
    24241 tgatctgggc ccgacggcca gcctttaccc ggccggtgag tggttccgcg tgcgcgatga
    24301 agccgagctg atccgcctgg tcgatgagcg cctggtggcc ggccgcgcag tgcccgagct
    24361 gcgcattgat cagagctgag tgtgggaggg gctttagccg cgactacagc cgctctatca
    24421 cttatcagaa gtcgccgcta aagcgcctcc cacagatgta accctcgtcc ttgagagggc
    24481 ggccttgtcg agttcacgcc ttgacttgtg ctggcgcgga tggtcatatc cgcgccgttt
    24541 caggtgtctc acgccgccgt gcgtgaggtt aaacgggaag tcggtgcgcc ctttgggcta
    24601 gtccgacgct gcccccgcaa cggtaagcga gcgaagtcat cacgatgcca ctgtgcagtt
    24661 tctgcatggg aaggcgatgg cttcatgacc ctcgcgagcc cggagaccgg cctgattctt
    24721 agggatgttg cgcgctgcca gtgatgcagc ggcaactccc aacagtttgg caacccgcgg
    24781 tgggcgggcg ttgacgccga tcgtgggtgc ctgcgcgccc gtgttttgct gttgcgcgtt
    24841 tccatcggga ttgccgtgcc tatccctttt gcgatggaag catcatggcg cgctatcccc
    24901 tgtctcaact ctctgtttcc cttgccttgt gcctggtcgg tacggctgtg gcgggcgagt
    24961 ccccggtcaa tttgccggcc acgaccgtgc aggccaaagc ggctgcggag ggcgaaagcg
    25021 atctgcatat gcccagcagc agcggttcgc gcctgggcct gacggccctg caaaccccgg
    25081 ccagcaccag cagcctgagc ggcgcacagg tgcgcgggcg caacaacctc accgtgcagg
    25141 acgcggtcac ccgcaccccc ggcatcagca gcatcggcag ccccggcaat ggcggcaccg
    25201 cactgtcggc gcgtggcttt accgggcaca gcgcgaccat gcagctgtat gacggcaccc
    25261 gccagtatgt cggtgccggc accgtgacct tcccggtcga cacctggtcg gtggagcgcg
    25321 tcgacgtgct gcgcggccct gcctcggtgc tgtacggcga aggcgcgacc ggcgcggtga
    25381 tcaacgtggt gccgaaaaag ccgtttagcg gcgccatcag caaccagctg cgcctcggct
    25441 acggcagcga tgaccgccgc caggccgcgc tggacagcgg cggctcgctg agcgatgcac
    25501 tgagctaccg cctcaacctc aatcagcagg ccagcaatgg ctgggtcgat ggtggcgagt
    25561 cgcagagcca ggcgctgagc gcagccctgc gctgggatgc cggcgatgcg ctgagtttca
    25621 ccctgtccca tgatcagggc gatcaggacc cgcagcgcta cctgggcaca ccgctggtcg
    25681 ccggccagta ccgcgagagc ctgcgcgagc gcaattacaa cgtcgataac gccgagattc
    25741 gctacaacga tcagatcacc cgactggtca gcgactggcg catcaacgac gccctgaccg
    25801 ccagtaacca gctctactac atcaagaccc agcgctactg gcgcaatgcc gagtcctacc
    25861 gctggcagcc gggcgatcag gtcaagcgca gtgacttcta cgagatcaag cacacccagg
    25921 agcaggtcgg cgaccgccag accttcaccc tggagcacgc cctgtttggc ctggatagcc
    25981 gcaccgtgat gggcgtggac tacaaccgca ttcacttcgc ccgccagcac gatttttcca
    26041 gcagcttcac tgacagcgtg ccgctcgccg gcagcggtgg cggtcagtat cagagcaccg
    26101 atccactgag gtacggcccg cgcgaacgca acctggcgcg gcagttctcg ctgttcgccg
    26161 agaaccgcac ccagctgact gagcgcctgt cactggtcag cggcgtgcgc cgcgatcagc
    26221 tgcatatcca gcgcgacaac ctgctgaccg acagcagtgc cgaccgcagc ctggccggcg
    26281 ataactggcg cgccgggttg gtcttcgagg ttacgcccga gctgtcgctg tacggccagt
    26341 acgccaccag caccgagggg gtgagcaacc tgctgacgct caatccgagc cagcagcagt
    26401 tcgacctgag cgaggcccag caaaccgaga tcggcctcaa gcaggcgttc tgggatggcc
    26461 agggcgaatg gacgctggcc gcttaccaca tcgtcaagga gaagctgctc agccgcgcca
    26521 ccccgaccgc gccgaccgag cagatcggcc agcagtcttc cgatggtctg gaagccaccc
    26581 tggagctggc gctggggcag ggttggcagg tgtcggccaa cgccgcattg gtgcgtgccg
    26641 agtacgacga gttccgcgag gccggtggtg accgcagcgg caatcggccg accaacgtgc
    26701 cacggcgcac cgccaacctg tggttgagca aggcgctgag cgagcaggtg caggcaggca
    26761 tcggcgcgcg ttatgtcgat tcgcgctacg ccgatacggc gaacagcgtg gcgctgccga
    26821 gctataccgt ggtcgatgcc aacattggct ggcaggtgca gccggctgtg cgcctgggcc
    26881 tggagctgaa caacctgttc gaccgccagt acgccatcac cggcagcagc gatggccagc
    26941 agtggtacct gggtgcgccg cgttcggtgt tcgtcactgc cgattaccgt ttctgatggc
    27001 gcgcttgcag atcagcgact tggcctggtc gcccaatggc gatcaggcct tgctcgacgg
    27061 cattgaactg gtggtgggcg acggtcagct ggtcggcttg ctcggcccca atggcagcgg
    27121 caagaccagt ctgctgcgct gcgcctaccg ttttcagcgg cccgatcagg gctctgtcga
    27181 gctggacggc gaagcgctgt ggagccgctc gccgcgctgg tgcgcgcagc gggtggcggt
    27241 ggtgttgcag gagtttcccc aggacttcgg cctcaccgtg gccgaagtcg ccgccatggg
    27301 ccgcacgccg cacaagggcc tgttcgatgg cgatgatcag gctgatgttg ccctggtcga
    27361 gcaggcattg gcgcgggtgg gtttgactga gcacaagcgc caggctttcg cccacctttc
    27421 cggcggcgag aagcaacgtg tgctgctggc ccgcgccctg gtgcagcagc cgacgttgct
    27481 gatcctcgac gagccgacca accacctcga cccgcgctat cagctggagc tgctgaggct
    27541 gatcaaaacc ctcggcctgg cgaccctggc cagctttcac gatctcaatt tggcggcggc
    27601 cttctgcgac cgcctgtatg tgctcgacca cggccgcgtg gtggccagcg gcaccccaac
    27661 cgaagtgctg acagaagcac tgttggccga ggtgtatggc gtgcaggcgc tggtcgaccg
    27721 tcatcccctc gcggggcatc cgcgtattac ctggatcaat ccatgaaacg ttttctgcgt
    27781 tacgtcctgt gtctgccgct gctgggcgtg gccctgtccg cccaggcggt taccgtcacc
    27841 agctgcgacc gtcaactgac catcgagcaa ccgccgcagc gcgcagtcag tcaggacatc
    27901 aacctgactg gcatgctcct ggccctcggg ctgaagtcgc ggatggtcgg ttacagcggc
    27961 atcagcgcct ggaaaacccc caaccccgag ctgatggcgc agctggccga tttgcccgag
    28021 ctggcggcgc ggcacccgtc actggaaaac ctgctgaacg ccgaggcgga tttcttcttt
    28081 gccggctgga actacggcat gcgcgtcggt ggtgaagtca ccccggccag cctggcgcgt
    28141 ttcggcatcc cggtgtacga gctgagcgag tcctgcgccc atgtcatgcc acgccgggtg
    28201 gccagcctgg aggatgtcta caacgacctg cataaccttg gccgcatctt cgccgtgcag
    28261 ccgcgcgccg aagcgctggt gcagggcatg cagcaacggg tggcggcggt gcgtgcacgc
    28321 ctggcgaaca gcggcccggc accacgggtg tttctctacg acagcggcga ggaaagcccg
    28381 tttaccgccg gccgcctggc catgccgcag gcgctgattg aggcagcggg tgggcgcaat
    28441 gtgatggatg agctggccgc gagctgggtg cgcgttggtt gggaagcggt gatcagcagc
    28501 gaccccgagg tgattctgat cgtcgattac ggcgagcgca gcgcggcgca gaagcgtgat
    28561 ttccttctac agcatcctgc cttgcagggg ctgacggcga tccgtgaaca gcgctttgtg
    28621 gtgctgccgt acctggcagt gacgccgagc ctggaaaacg ccgccgccat cgaaaccctg
    28681 gctgcggcgc tgcacccgca ggcgttcggc caatgattcg cagcccgcgc gcctaccgct
    28741 tgttgctgct ggtgctggcc ttggcgctgg cgctgtcctg tgccgcgtca ttgggctttg
    28801 gcgccgcgcc agtgccactg ccgcgcgtgc tggaaatcct cggccagcgc ctgcttgact
    28861 tgcagccggc gcaggcggtg agcagcgccc aggacagcat cgtctggctg atccgtgcgc
    28921 cacgggtggt gctcggtgca ctggtcggtg ccgggctggc gctggtcggc acggcgctgc
    28981 aggcggtcac gcgcaacccg ctggccgacc cgcatctgct cggggtcagt tccggtgcgg
    29041 cgctcggcgc ggtgattgtg gtgctgtatc tgggggaatt tatcggcctg ctcagcctgc
    29101 cgctggcggc ctttgtaggt gcgctggtga gcatgctgct ggtgctggcg attgccagtc
    29161 gcggcggccg gcttgagagc gaccgcctgc tgctggccgg ggtggcggtg tcgtttgtga
    29221 tgatggcggc gagcaacttg ctgctgtttc tcggcgatca ccatgccgcc agttcggtga
    29281 tcttctggat gctcggcggc ctcggtctgg cgcgctggga actgctctgg ctgccggcgc
    29341 tgtgcctggt gttggccttg ttgctgttgc tcggtctggc gcgggcgttg aatgcgctga
    29401 tggctggcga acacagcgcg gtcagcctcg gtcttgagcc gcgacgggtg cgcctgctgg
    29461 tgtttgtttg tgcctcgctg ctgaccgggg tgctggtgtc gctcagtggt gccatcgggt
    29521 ttgtcgggct gatcctgccg catgtggcgc gctttctggt gggcgccgag catcgtcgcc
    29581 tgctgccggt cagtgccttg cttggcgcgc tgtttctggt ctgggtggat gtcgccgcgc
    29641 gcaccctgct ggcgccgcaa gacctgccca tcggcatcgc tacggcggcg attggcgggg
    29701 tgtttttcgt gcttttactg cgtcggcgct gaatcgctaa tgtttttttc agcgctgctg
    29761 taacgcctgt tcgtctcgtc gctctagttg aatgccggca cacaaaaccc ttttcgtccg
    29821 gccaacccag atcacccgag gatacgcaca tgaccatgaa atccaccgct gctactggtg
    29881 ctgccattgc cttcgctgct gccagcctgt tcgctggtct ctcaagcacc gtagcagtgg
    29941 ctgaagaagc caaagtacat tgttacggcg tcacgtcctg caaaggccag aatgactgca
    30001 agaccgccga aaacagctgc aaaggtcagg gtgcctgcaa aggtcacggc ttcaagtcga
    30061 tgaccctcgc tgagtgcaat gccgccaaag gtactgtcgg cgagtaagtg agccggggcg
    30121 gccgccgcgc ggttgccccg catcaccctg caaggagcca gcaatgaatg ctcaacgcgg
    30181 caatctgggt tttggcctgg gcctgcgcag tacctattac caagccattc tcgagcagca
    30241 gccggccatc gactggttcg agatcgtctc ggaaaattac ctggtagcgg gcggcaaggc
    30301 cctctacttt ctcgatgcca tcggcgagca gtacccgctg gtgatgcatg gtgtgtcgct
    30361 gtcgattggc ggcccgcatg agctggaccg tgactacctg cgccagctca aacacctcgc
    30421 tgcgcgcgtg cagccggcgt ggatctccga tcacctgtgc tggagccggg gcaatgccca
    30481 ccagctgcac gacctgctgc cgctgcctta taccgaggag agcctgcagc atgtggccga
    30541 gcgtgtgcgc caggtgcagg acgtgatcga gcggccgctg gtgctggaaa acgtctccag
    30601 ctacctgcgc tgtgccgatg atcagttcag cgaatggcag ttccttgctg cactcagcga
    30661 gttgagcggc tgcgagctgt tgctggacgt caacaatgtc tacgtcagcg cgcgcaacca
    30721 tggctttgat gcctggacct ttatcagtag cctgcccaag cagcgtatcc gtcagctgca
    30781 cctggccggc cacagcgatt acggccgcta tctgatcgac acccatgatc agccggtcag
    30841 cgatccggtc tggcagcttt atcaacgcac cctgcagcac ctcggcccgg tgtccacgct
    30901 actggagcgc gacgatcatt tccccgatct gggtgtgctg ctcgacgagc tgaataccgc
    30961 gcgccggttt gccgcccaag cactgcaagg cacagcgcaa tgcgcctgag tgactggcag
    31021 gcgcaggtgc agacctacct gcagggcccc gatgcccagc ccaatccggc gctgcaggcc
    31081 agtctgctgg gcagtgcggc gctgagtgcc gagcagggcc tggcgattta ccacaacgct
    31141 taccgcgcgc gcctgctgga ggccttgcgt ggtgactatc cggcggtgca tggctggctc
    31201 ggtgatgagg aattcgatgc cctggccctg gcctatctcg acgcgcaccc ctcgcagcat
    31261 ttcagcctgc gctggttagg cgcacagctg gcggacttta tcgacggcta cctgattccc
    31321 gcacaggccg cgccgctcag cgaactggcg cggctggaat gggcctttac cctggccttc
    31381 gatgcgccag aaggccagcc cctgagcctg acgcagatgg ccgaactgcc ggctgaagac
    31441 tggccgaccc tgcaggtgcg tttgctaccg agcgtgcagt ggctggcgtg ccgacataac
    31501 agcctggcga tctggcgtgc gagcaaggag cagggtgact tccctggcag ccagccactg
    31561 gttgcgacag agacctgtgt ggtctggcgt gatcagctga ttacccgcta ccgcagcctg
    31621 gcggccgatg aagcggcggc attgcagggc atgactgtgc agggttggac cttcgctgag
    31681 ctgtgcggcg agctggcaca cctgggtgaa caggcgccgg cgcaagcggt gagctggttg
    31741 cgtcagtggc tggcggatgg gctgttgcag cgggcggatc aagcccagcc gtgatgatct
    31801 ctgctctgtc ggcacatacc ctgacagggc attttccttc aataccctgc gcggtgggcc
    31861 tgtcagcctg gcacctcctt tgtggtgctg acaggtgaat gatgaatacg ctgttttatt
    31921 ccatggcggg ctttgccctc gctgcctcga tttctcccgg cccggtcaac ctgctggcgc
    31981 ttagcgccgg cgcgacccat ggcctgcgcc gcagcatgcg tcacgtcacg ggtgcgacgt
    32041 tcggctttac cgcgctgttg ctggccatgg gcctgggctt gcagcagctg ttgcagcagt
    32101 ggccggcgct aacgcacgga gtggcctggt ttggcgtcgc ctttctgctg tacatggcct
    32161 acggcctggc ccggtctgac gggcagctga gtggcaacgc cgcgcagcag cgcccgtcgc
    32221 tgttcaaggg cgcgctgatg cagtggctca accccaaggc ctggctggcc tcgatggcag
    32281 gcatgggcgc ctatgcggcg gatggtgatc tgtggcgggt ggtgcagttc gcggcgctgt
    32341 atttcgtgat ttgttatctg tcgatcagtt gctgggcgtt ggccggggtt tacctgcgcc
    32401 agtacctgga gaatcccctg tacctgcgcc ggttcaatcg cggcctggcc ttgctgctgg
    32461 tgctctcggc ggtttatctg ctggtctgat caggcgcggc ggtattgccc gggggtggcg
    32521 gcgagcagct gtttgaaggt gcgctgcaag tgcgcctggt cggcaaagcc ggcgtccagc
    32581 gccacctcgg caatcgactc gccgcgtttc agcccggcct gggccacttg cacgcggcag
    32641 ttgagcaggt aggcatgtgg ggtcatgccg tagtgctggc ggaaactgcg gatcaggtag
    32701 gagggcgata gctcggcggc gctgcagatg tcttccagct tgagcgtctg ggtgcagtgc
    32761 gcgcggataa attccgcggc cagacggatg cgcgggttgt cctcgcggat gggtgccggc
    32821 gcgggtgcca ggcgctgttg cagggtgctg aaatagtcga tcagggcgca ctctttgcgc
    32881 agctgatcga ctgatgccgc gttgagtagg tcgtacagct ggttgaggcc gttgaacagc
    32941 tcagggtcgc ggctgaggat ggcggagaag ggctgcaggt gcgggttgtg gctgaagccc
    33001 agctggtgtt gcaggtcgcc cagccaggtg ctgtcgatgt agaacatgcg atacgaccag
    33061 ggctgcccat caacggggtt gcaggcatgc acgtcacccg ggttcatcag caccagcgtg
    33121 ccggccgcga cctgctcgcg cgccttttca ttgaggtagg tgctgcggcc ttgggtgatg
    33181 gtgccgatcg aaaaacactc atgggaatgc ttggcgtagc acaacagacg gccatcaagg
    33241 gcttcgcgcg tttcgacgaa gggcagcgct gggtcgcgcc agaagcgtgg ggtggtggcc
    33301 tgttgctcag tgctcatcat gcgtccctgg cgtaatcgcc gtatagccag cgctgactat
    33361 agcggccgct ggcgcttgtt caaacagtcg agccgctgcg ctctgcacgg cgcggtgctt
    33421 tgtcttacca tgccggctcc gccttgttgc gagccgcgca tgtctgcctt tctgctgcgt
    33481 taccgcgttg tcctgatcgt cctgctgatt ttgctcggcc tgctggcgag catctggctg
    33541 gccgggcgtt atgccgagca gcgcgcctgg caggagcgca gtctggaggc gcgcgggcag
    33601 ttgcagctgt atgcgcagtc gatccacacc ctggtcgaac gcttccgctc ggtgcccgaa
    33661 gtgctggcgc tggacagcga tatcaagtcg ctgctgcgtg cgccggatga cagcttgctg
    33721 cgcagcacac tcaacgagcg gttggagcag ctcaatacgg ccgccggctc caatgtgctg
    33781 tatctgctca acgcgaaagg cgaaaccctg gtggccagca actggcgcga ctggagcagt
    33841 tttgttggca acaactacgc ctttcgcccg tattttcagg atgccgtgcg ccagtcctcc
    33901 gggcgctatt ttgcggtggg cgtgaccact ggcatccccg gctattttct ctcccatgcg
    33961 gtgcgcgatg acaacggcgt ggtgctcggc gtgctcgtgg tcaagctgga gctggaagag
    34021 ctgcagcgcg aatgggccag ccagcctggc gtgctgctgg tggccgatag ctactcggtg
    34081 gtgatcctca gcaaccgtcc ggcctggcgt tttctcgccc tcgacagttt gagcgagcag
    34141 gcccgcgccg agctggtgga ggtgcgcaaa tacgccgaac aggcattgca gccgctggcc
    34201 agcacgctgc gccggcagat cgacagcgac agtcagtggc gccgggtcga gggtccggac
    34261 ggcccgcgtg actacctctg gcagcgtcag gcattgggtg atgaaggctg gaccctgcac
    34321 ctgctgcacg agccaaaaac tctggtcgac agcgtgcgca gctatcgcct ggccgccgcc
    34381 ggggtgtgga tgaccctggc ctttctgctg ctctacctgg cccagcggcg taagactcag
    34441 cggctgcagg ccggtattcg cgagcgcctg gagcgtgaag tggcgctgcg caccgccgag
    34501 ttgcgcgaag cccaggaagg cctggtgcac gcggcgaaga tggcggcgct ggggcagatg
    34561 tccgcggcca tggcccatga aatcaatcag ccgctgactg ccatgcagat gcagctgggc
    34621 agcttgcgcc tgttgctgga tagcggtcgc caggatgacg tgcgcgaggg gctgcaacgc
    34681 atcgatggcc tgctgcaacg tatcgccggg ctcactggtc atctgaaaac ctttgcccgc
    34741 aaaagcccgg cggggctgag cgagcgtctg cggttgagcg atgtgctgga gcaagccttg
    34801 cagctgttgg cgccgcgcct gcgcagcgaa caggtgcagc tgcacagcga gatcgacacc
    34861 gaggcgcggg tgctgggtga cgcgattcgc ctggagcagg tgctgctcaa tctgctgaat
    34921 aacgccctgg atgccatggc ggacagcacc gtgctgcaac tgcagatctg tatcgagcgt
    34981 caggccgagc agtgtctgct gagtatcgcc gacagtggcg gcggaatcgc tgccgaagcg
    35041 ctggatcatg tgttcgagcc gttctttacc actaaaccgg tgggcgccgg gctgggtctc
    35101 ggtttggcgg tgtcctacgg catcgtccgt gagctgggcg gtagcctgga ggcggccaat
    35161 ggcgagcagg gtgcggtgtt taccctgcgt ctgccggcag cgccgaatgc gtagtgcggg
    35221 ttatttcccg gattttatcc gggctacact gccgccgatt gatcaggagg tgatatgacc
    35281 gggcacgtaa tagtcgtcga cgatgaagcg gcaatccgcg aggcggtgca gcagtggctg
    35341 gagctgtctg gctttcgcgt gcacagctgc gccacggcgc aggccgcgct ggcgctggtc
    35401 gaccgtgact accccggcat catcatcagc gatgtgcgca tgcccggcac cgatggcatg
    35461 cagctgctcg acagcgtgct ggagcgcgac cgcgacttgc cggtgattct gatcaccggg
    35521 cacggcgacg tgccgatggc ggtgcaggcg ctgcgccaag gcgcgtatga ctttatcgaa
    35581 aagcccttta cccccgaacg cctgctcgac agcgtgcgcc gcgcgttgga caagcgccgc
    35641 ctagtttgtg agaaccgcca gctgcgtcag cagttcgccc tcaaggacca tatcgaggcg
    35701 cagcttttgg gcgtctcgcg gccgatggaa accctgcgtc ggcagatcct cgaactggcc
    35761 ggcacctcgg tgaatatcct cctgcgcggc gacaccggca gcggcaagga gcgggttgcc
    35821 cgttgcctgc atgatttcag caaccgcgcc ggcaagccgt ttgtggcgct gaactgcgcg
    35881 gcgattcccg agcacctgtt tgagagcgag ctgttcggcc atgaaagcgg cgcctttacc
    35941 ggcgcccagg gcaagcgcat cggccgcatc gaacatgccg acggcggcac gctgtttctc
    36001 gatgaggtgg aaagcctgcc gctggcccag caggtcaaac tgctgcgtgt gctgcaggag
    36061 aaaaccctcg agcgcctggg ttccaatcgc agcatccagg tcgatctgcg ggtgatcagc
    36121 gcggtcaagc cggacctgct cgatgaggtc aaggctgggc gtttccgtga agacctctac
    36181 taccggctga atgtcgccac cctgcggatt ccgccgctgc gtgaacgccg tgaagacatt
    36241 ccgctgctgt tcgaacactt cgccaaccag gctgcgcaac gccatggccg tgagacaccg
    36301 agcctgccgc ccagcgagct gacccggctg ctcgggcacg actggccggg caacgtgcgc
    36361 gagctgatca acgccgccga gcgccatgcc ctgggcttga gtggcgaagt ggagcgtggc
    36421 gagggtctgc cggtgcaatc gctggccgag cagatggaag ccttcgaggc gcaatgcctg
    36481 caccgcgccc tgcagcactg ccagggcaat atcgccgagg tgattgcgct gctgcagctg
    36541 ccgcgccgca ccctcaacga gaaaatgcag cgccatggtc tgcagcgcag cagctatcgg
    36601 cccgctggta gcagcgacga agattgaaag cacgcgcaca gctcgtagcg aataagcctt
    36661 ggcgcaatcc gggatcgccc tgcgcttatc cgggctacat gtgacgtagt cggcggaatt
    36721 tcgcttatct gagttttttc actggcggat ttccgcttac tacagccgca aggctggcag
    36781 tgctgattgg agcaacggct ctagtacggg cttttctctc actggcacgg catctgctat
    36841 agctcttgtc agccgggcac tcgattgcct cggcctctat aagaaagaca agaggtgaat
    36901 cccatgcact gcccagcatc atctgcgcgc ttgtcgcgtt cggcatcaac cgcccatctc
    36961 ttgtgcgctc ggctcaggcc gcgcgtgagc ctctgctcct tttcgccatt gcgttagtcc
    37021 cggcgctgca ccgccgcctg ggtggcgcgc agcgtctaca gtgaaaacct ctgcataaag
    37081 ccataacaac gacggagatt cttccatgcg aatgaccaaa cgtttcagcc tgtttgctgc
    37141 tgccgcagcc tttaccgcga gcaccgcggt actcgccgcc ccgaccttta tcaatatcct
    37201 taccggcggc accagcggtg tgtattaccc gattggtgtg ggcctgtcgc agctgtatag
    37261 caacggcatc gaaggctcga agacctcggt gcaggccacc aaagcttcgg tggaaaacct
    37321 caacctgctg caagccggtc gtggtgagct ggccctggct ctgggtgact ccgtggccga
    37381 tgcctggaat ggcgtggaag acgccggctt caaggcaccg ctgaaaaagc tgcgggcgat
    37441 tgcgggcacc tacccgaact acatccagat cgtcgccagc aaggaatccg gcatcaccac
    37501 cctggctgac ctgaagggca agcgcatctc cgtcggcgcg ccgaaatccg gcaccgaact
    37561 gaacgctcgc gccatcttca aggccgctgg actgagctat gaagacatgg gcaaggtcga
    37621 gtttctgccg tacgccgagt cggtcgagct gatcaagaac cgtcagctgg atgccaccct
    37681 gcagtcctcg ggcctgggca tggcggcgat tcgtgacctg gccgcgaccc tgccgatcac
    37741 cttcgttgcc atcccggcgg agatcaccgc gaagatcgac aacgccgcct accaggctgc
    37801 cgcgatccca gccggcacct acgacggtca ggacagcgat gtaccgaccg tggccatcca
    37861 gaacatcctg gtcagccatg acggtgtgtc cgaggaagtg gcctaccaga tgaccaaact
    37921 gatgttcgac aacctcgagc gcctgggcaa cgcccactcg gcggccaagg atatcaagct
    37981 ggaaggtgcc gccaagggcc tgccgattcc gctgcatcca ggtgccgagc gcttctacaa
    38041 agaagccggc gcactgtaag gctgctgaag ggttggctgc gtaggatggg ttgagcatcg
    38101 cgatacccat gctgcacggt tggagcctgg tgaaagattt cactattgct cgatgggtta
    38161 cgccgctgcg cggctaaccc atcctaccca ggcatcactg tcctagagct gcgaataaaa
    38221 ccggcgtgca gggcacggca ggttttgttg gcgactcttg tgctatcgac gttgtaacga
    38281 gggtgtgacc ttcatgagtg agcaaaacca aggcctcgcc gccaacccca gcgattggcc
    38341 gaaagcgctg ttttatgtcg cgctgctgtt ttccatcttc cagatcatca ccgcggcctt
    38401 ccacccggta tccagccaga ttctgcgtgc cgtgcatgtg ggctttctgc tgctggtggt
    38461 gtttctctgc tttcccgccc gtggcaagga gcagccctgg cagccgatgg cctggctact
    38521 cggcctggcc ggtatggcca cggcgttcta ccagtggtat ttcgaagctg atctgattca
    38581 acgctccggt gatctgaccc agagcgacat ggtcgtcggc ctggtgctga tcgtgctggt
    38641 gtttgaagcc gcccgcctgg tgatgggcat cgccttgcca tttatctgcg cgctgtttct
    38701 cgcctacggc ttgttcggcc aatacctgcc gggcgacctg atgcaccgtg gctacgccat
    38761 cgaccaaatc gtcaatcagc tggccttcgg caccgaaggg ctgtacggca cgccgaccta
    38821 cgtatcggcc acctatatct tcctgtttat cctgtttggc gcgttcctcg aacaggccgg
    38881 gatgatcaag ctgttcaccg acttcgccat gggcctgttc ggccacaagc ttggcggccc
    38941 ggccaaggtg tccgtggtgt cgtcggcgct gatgggtacc atcaccggtt cgggcgtggc
    39001 caacgtggtc accaccggcc agttcaccat tccgctgatg aagcgcttcg gctacaagcc
    39061 ggcgttcgcc ggcggcgtgg aagcgacctc gtcgatgggt agccagatca tgccgccgat
    39121 catgggcgcg gtggccttta tcatggccga gacgatcaat gtgccgttca tcgaggtggc
    39181 caaggccgcg ctgatcccgg cgctgctgta cttcggttcg gtgttctgga tggtccatct
    39241 ggaagccaag cgcgccaacc tgcagggcct gccgaaagac cagtgcccga atccctgggc
    39301 ggcgatcaaa gagcgctggt acctgctgat tccgctattc atcctgatct acctgctgtt
    39361 ctccggacgc acgccgctgt tctccggcat ggttggtctg gccctgaccg ccatcgtgat
    39421 tctcggctcg gcgatcatcc tgcgggtgtc gtccaaggtc atgcgcttcg ccttctggat
    39481 tgctctgggc gtgctctgcg ccggcttctt ccagctcggc atcggcgtgg tgttcggcgt
    39541 cgtcgcgttg ctggtggcaa tctgctggtt tatcaaaggc ggccgcgaca ccctggtgat
    39601 ctgtctgcat gcgctggtgg aaggtgcacg ccatgcggtg ccggtgggta tcgcctgtgc
    39661 cttggtgggg gtgattatcg gtgtggtctc gctgaccggg gtggcttcca ccttcgccgg
    39721 ctatatcctt gccatcggcc aggacaacct gttcctctcg ctgatcctca ccatgctcac
    39781 ctgcctggtg ctgggcatgg gcatcccgac cattcccaac tacatcatca ccagctcgat
    39841 tgccgcgccg gcgctgctgg agctgggcgt gccgctgatc gtctcgcata tgttcgtgtt
    39901 ctatttcggc atcatggccg acctcacccc accggtggcg ctggcctgtt tcgcagccgc
    39961 accgattgcc aaggagagcg gcctgaagat cagcctgtgg gcgattcgca tcgccgtagc
    40021 cggtttcgtg gtgccgttta tggcggtgta cgagccagcg ttgatgctgc agggcgatag
    40081 cctgctggcc acgctctatg tggtgctcaa ggcactgctg gccatcggca tctggggcgc
    40141 ggtgtttacc ggttacctgc aggccaaact gagctggtgg gaacgcgcgc tgggctttgc
    40201 cgccggtgcc agcctgattc tggccacgcc catgagtgat gaaattggct tcgccctggc
    40261 ggcgctgttt atcgcccagc acgtctggcg ggcccatcgc gcgcaggtgg ctgctgcgtg
    40321 attggcctgt gcctgggttt ggctggcgcg gtatgggcag aagtgcccac gcccagcttt
    40381 accctggcct ggacgcacac gattgagaag attcgttggg aagaggatta ccgcgtgacg
    40441 ccagagggat tgctcctcgg cgaagcgcgg gtcaagggtt ccggggccgg tatggagatt
    40501 ccagacggtg ccgaactacg cgagggcagc tggcattacc agcgtcagct atcacccttg
    40561 caaccactgc gtttggggcg aacccccgag gcaggggatt atcaactgtg tttcaaccag
    40621 cgttgccact cgatgagtga atggctcggc ccgccacaag cgacccagcc ggcgttggaa
    40681 ctctggagct gtgag
//
LOCUS       DASRUM010000247         5670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_464679, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000247 DASRUM010000000
VERSION     DASRUM010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464679"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="VFF22_16365"
     CDS             <1..129
                     /locus_tag="VFF22_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HZX18425.1"
                     /translation="RAAENALNEKGAEFDYGSAAAHLAEVAAQLRTVQQLRKKFGG"
     gene            260..1621
                     /gene="glmU"
                     /locus_tag="VFF22_16370"
     CDS             260..1621
                     /gene="glmU"
                     /locus_tag="VFF22_16370"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HZX18426.1"
                     /translation="MSLDIVILAAGQGTRMRSALPKVLHPVAGKSMLGHVIDTARLLK
                     PQGIHVVIGHGAELVRERLAADDLNFVLQSEQLGTGHAVAQALPALSAERVLILYGDV
                     PLIEVETLQRLLQQVSEQQLGLLTVNLNDPTGYGRIVRDDNGVVKAIVEHKDASAEQR
                     LISEGNTGILAVPGKKLGDWLGRLSNSNAQGEYYLTDVIAMAVADGLVVATEQAADEM
                     EVLGANDRIQLSQLERHYQYRAARRLMVQGVTLIDPARFDLRGEVTVGRDVSIDINVI
                     LEGQVVIEDGVQIGPNCVIKNSILRKGAIVKANSHLEGAEMGEDADCGPFARLRPGSV
                     LGAKAHVGNFVELKNAKLGEGAKAGHLSYLGDAEIGARTNIGAGTITCNYDGANKFKT
                     VLGEDVFIGSNSALVAPVTLGDRATTGAGSVITSDVPDNTLAVGRAKQRNIEGWKRPT
                     KKT"
     gene            1636..2145
                     /locus_tag="VFF22_16375"
     CDS             1636..2145
                     /locus_tag="VFF22_16375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZX18427.1"
                     /translation="MPLRKRWLIPALFLICALLPEWPQIPVQGASARDWNPRTFWFEP
                     WGRSGVHKGIDIFAKLGTPVRSSSYGVVLFRGEIAQGGKVVVALGPKWRIHYYAHLQT
                     IDAYPGQPLLAGSLLGTVGDTGNAQGKPAHLHYSIVSLLPLPWRADESTQGWKKMFYL
                     DPNQLLQAR"
     gene            2244..3011
                     /locus_tag="VFF22_16380"
     CDS             2244..3011
                     /locus_tag="VFF22_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HZX18428.1"
                     /translation="MSKRNTPQRRHTILALLAEQGEVSVDELSKAFSTSEVTIRKDLA
                     ALEKSGLLLRRYGGAVPMPQELISDIQPISQYKQAIARAGVARIREHARIIIDSGTTT
                     AAMIPQLGHKPGLVVMTNSLNVARAISELEHEPVLLMTGGTWDPHSESFQGQVAEQVL
                     RSYDFDQLFIGADGIDLNRGTTTFNELLGLSRVMADVAREVVVMVESDKIGRKIPNLE
                     LPWSGIHTLITDERLSPEAREQLTERGIKLICAAVEA"
     gene            3022..4872
                     /gene="glmS"
                     /locus_tag="VFF22_16385"
     CDS             3022..4872
                     /gene="glmS"
                     /locus_tag="VFF22_16385"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZX18429.1"
                     /translation="MCGIVGAVAERNITAVLVEGLKRLEYRGYDSAGVALLTDHNGLD
                     RRRRVGKVSELESALSEEPLAGRLGIAHTRWATHGAPSERNAHPHFSSHELAVVHNGI
                     IENHEELRAQLKGLGYVFSSDTDTEVIVHLLDHKLKTQPDLTAALKEAVKELHGAYGL
                     AVISAKQPDRLLAARSGSPLVIGLGLGENFLASDQLALRQVTDRFMYLEEGDIAEIKR
                     DSVQIWSVDGQPVTRESVQYHEGAEAADKGEYRHFMLKEIFEQPKVVQRTLEGRLGSD
                     HVLVQAFGPQAAELFAKVRNVQIVACGTSYHAGMVARYWLEGLAGIPCQIEVASEFRY
                     RKVVVQPDTLFVSISQSGETADTLAALRNAKEHAPGQGGYLASLAICNVGISSLVRES
                     DLTLLTQAGPEIGVASTKAFTTQLVALMLLTLSLGQVRGTLDKGLEAELVDELRRLPT
                     RLGEALAMDSVVEKVSELFAEKHHSLFLGRGAQYPVAMEGALKLKEISYIHAEAYPAG
                     ELKHGPLALVDSDMPVVTVAPNNELLEKLKSNLQEVRARGGELVVFADEQAGMSNGEG
                     THVVNMPHIHDALAPILYTIPLQLLSYYVAVLKGTDVDQPRNLAKSVTVE"
     gene            4887..5117
                     /locus_tag="VFF22_16390"
     CDS             4887..5117
                     /locus_tag="VFF22_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017734218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18430.1"
                     /translation="MKREDIKSKQAQGVLFDTHIIANPANTREWIVLLKKGAGTSFFL
                     VDGQEQVESFADLNGLIEELRLLGIKGAEVHL"
     gene            5143..>5670
                     /locus_tag="VFF22_16395"
     CDS             5143..>5670
                     /locus_tag="VFF22_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HZX18431.1"
                     /translation="MPIRYARSLTGRTRSVAANRHLGFALAFVAGAINAGGFLAVQQY
                     TSHMTGIVSAMADNIALGAYGLVWSGLGGVLSFLLGAACSTVMINYSRRRQLHSEYAL
                     PLLLEAALLLCFGFLGASLAQITGLFVPLTVMLLCFIMGLQNALITKLSRAEIRTTHI
                     TGIITDIGIELGKLFY"
BASE COUNT         1112 a         1708 c         1749 g         1101 t
ORIGIN      
        1 cgggctgccg agaatgcctt gaatgagaag ggcgcggagt tcgattacgg ttccgcagcc
       61 gctcacctgg ccgaggtcgc ggctcagctg cgtaccgttc agcagttgcg caagaagttc
      121 ggcggttaat tccgacgctt catcgcaaac gagtaaaagg gtagccttgg ctaccctttt
      181 tctttgcctg ctgtttttac cttttcagtt ggccgggcta gggatttagc ccgccttcat
      241 ctgcctggaa tccgctctta tgtcgctcga tatcgttatc ctcgccgccg gccaaggcac
      301 ccgcatgcgt tccgccttac cgaaagtcct gcacccggtg gccggcaaat ccatgcttgg
      361 ccatgtgatc gacaccgccc gtctgcttaa accgcaaggc attcatgtgg tgatcggcca
      421 tggcgcggaa ctggtgcgcg agcgactagc ggcggatgac ctgaatttcg tgctgcagag
      481 cgagcagctc ggcaccggcc acgcggtggc tcaagcgcta ccggcgttga gcgctgagcg
      541 tgtgctgatt ctctacggcg atgtgccgct gatcgaagtt gaaaccctgc agcgtctgct
      601 gcaacaggtc agcgagcaac aactaggctt gctcacggtc aacctgaatg acccaaccgg
      661 ctacggccgc atcgttcgtg atgacaacgg cgtggtgaaa gccatcgtcg agcacaagga
      721 cgccagcgcc gaacagcgcc tgatcagcga aggcaacacc ggcatcctcg ccgtaccggg
      781 gaaaaaactg ggcgactggc tcggtcgctt gtccaacagc aatgcccagg gtgagtacta
      841 cctgaccgat gtaatcgcca tggccgtggc cgatggcctg gtggtggcca ccgaacaggc
      901 ggctgacgag atggaagtgc tgggggccaa cgaccgcatc cagctttcgc agctggagcg
      961 tcattaccag taccgcgccg cgcgccggtt gatggtgcag ggcgtgaccc taattgatcc
     1021 ggcgcgtttc gacctccgcg gcgaagtcac agtaggtcgt gatgtgtcga tcgatatcaa
     1081 cgtgatcctc gaaggccagg tggtgatcga ggatggcgtg cagatcggcc ccaactgcgt
     1141 gatcaagaac tcgatcctgc gcaaaggcgc catcgtcaaa gccaacagcc atctggaagg
     1201 tgccgaaatg ggcgaagatg ccgattgcgg cccgtttgct cgtctgcgtc ccggttcggt
     1261 gctgggcgcc aaggcccatg tgggtaactt cgtcgaactg aagaacgcca agctgggcga
     1321 gggcgccaag gccggtcacc tgtcttacct gggcgatgcc gaaatcggcg cgcggaccaa
     1381 tatcggcgcc gggaccatca cctgcaatta cgacggcgcc aacaagttca agaccgtact
     1441 gggtgaggac gtgtttatcg gctccaacag cgccctggtc gccccggtga cgctgggcga
     1501 ccgcgcgacc actggcgccg gctcggtgat caccagcgat gtgccggata acaccctggc
     1561 cgtcggccgc gccaagcagc gcaatatcga aggctggaag cggcctacca agaagacctg
     1621 atggagttgg caaggatgcc tttacgcaaa cgttggttaa taccggcgct gtttcttata
     1681 tgtgccttgc tgcctgagtg gccgcagatt ccggtgcagg gcgccagtgc ccgggactgg
     1741 aatccgcgca ccttctggtt tgaaccctgg ggccgctcgg gcgtgcacaa gggcatcgac
     1801 atcttcgcca aactcggcac gccggtgcgc tccagtagct atggagtggt gctgtttcgc
     1861 ggtgagatcg cgcagggcgg caaggtggtg gtcgccctgg gaccgaaatg gcgcattcac
     1921 tattacgccc atctgcaaac cattgatgcc tacccaggcc aaccgctgct ggctggcagt
     1981 ctgctgggca cggttggcga tacgggtaac gctcaaggca aaccggcgca cttgcattac
     2041 tcgattgtca gcctgctgcc gttgccttgg cgggcggacg agtcgaccca aggctggaag
     2101 aagatgtttt atttggaccc caaccagctt ctgcaagcgc gctgaattgg cctggaccta
     2161 gctgccctac gtcgattgct gcttgacgaa aaagcttcat taggttttga ttcgcgccat
     2221 tatctttcga atcgaaactt agcatgtcga aacgcaacac tccgcagcgt cgccacacca
     2281 ttcttgccct gctcgccgag cagggcgagg tgagtgtgga tgagttgtcg aaggcgtttt
     2341 ccacctccga agtgaccatt cgcaaagacc tcgccgcctt ggaaaaaagc ggcttgctgc
     2401 tgcgtcgtta cggcggtgcg gtgcccatgc cgcaggagct gatcagtgat atccagccaa
     2461 tctcgcagta caagcaggcc atcgctcgcg ccggtgtggc gcgcatccgc gaacatgcgc
     2521 ggatcattat cgatagcggc accaccacgg cggcgatgat tccgcagctc ggccacaagc
     2581 ccggcctggt ggtgatgacc aactcgctca acgtcgcccg tgctattagc gagctggagc
     2641 atgagccggt gctgcttatg accggcggca cctgggaccc gcattccgag tcattccagg
     2701 gccaggtcgc cgagcaggtg ctgcgctctt acgacttcga ccaactgttt attggcgccg
     2761 atggcatcga cctgaatcgt ggcaccacca ccttcaatga attgctcggg cttagccggg
     2821 tgatggcgga tgtggcccgc gaagtggtgg tgatggtcga gagcgacaag atcggccgca
     2881 agattcccaa cctggagctg ccatggagcg gcatccatac cctgataacc gacgaacgcc
     2941 tcagccctga ggcccgtgaa caattgaccg agcgcggcat caagctgatc tgcgcggctg
     3001 ttgaagctta aggagaagat tatgtgtggc atcgtaggcg cagtcgccga acgcaatatc
     3061 accgccgtgc tggttgaagg cctcaagcgc ctggaatacc gcggttacga cagcgccggc
     3121 gtggcgttgc tgactgatca taacggtctc gaccgtcgcc gccgtgtcgg caaggttagc
     3181 gagctggaaa gcgccttgag cgaggagccg ttggccggcc gtctaggcat tgcccacacc
     3241 cgctgggcca cccacggtgc accgagcgag cgtaacgccc acccgcattt ttccagccat
     3301 gagctggcgg tggtgcacaa cggcattatc gagaaccacg aagaactgcg agcccagctc
     3361 aagggtctgg gttacgtgtt cagctccgat accgataccg aagtgattgt gcacctgctc
     3421 gaccacaagc tgaaaactca gccggacctg accgcagcac tgaaagaggc ggtcaaggaa
     3481 ctgcacggtg cctacggcct ggccgtgatc agcgccaagc agccggatcg cctgctcgcc
     3541 gcccgcagcg gtagcccgtt ggtgatcggt ctgggcctgg gtgagaactt cctcgcctcc
     3601 gaccaactgg ccctgcgtca ggtcactgac cgctttatgt acctggaaga aggcgatatc
     3661 gccgagatca agcgcgacag cgtgcagatc tggagcgtcg acggccagcc ggtgacgcgc
     3721 gagagcgtgc aatatcacga aggtgcggaa gccgccgaca agggcgaata ccgccacttt
     3781 atgctcaagg aaatcttcga gcagcccaag gtcgtgcagc gcaccctgga aggccgtctg
     3841 ggttctgacc acgtgctggt gcaggccttc ggcccgcagg ctgccgagct gttcgccaag
     3901 gtgcgcaacg tgcagatcgt cgcctgcggc accagttacc acgccggtat ggtcgcccgt
     3961 tattggctgg aaggcctggc cgggattccc tgccagatcg aagtagccag tgagttccgc
     4021 taccgcaagg tggtggtgca gccggatacc ctgtttgtca gcatctccca gtccggtgaa
     4081 accgctgaca ccctggctgc cctgcgtaat gccaaggagc atgcgcctgg ccagggcggt
     4141 tacctggcca gcctggccat ctgcaacgtc ggcatcagct ccctggtgcg cgaatccgac
     4201 ctgaccctgc tgacccaagc cggcccggaa atcggtgtgg cttcgaccaa ggccttcacc
     4261 acccagctgg tcgcgctgat gctgctgacc ctgtccctcg gtcaggtgcg tggcactctg
     4321 gataaaggcc tggaagccga gctggtcgac gaactgcgtc gtctgccaac ccgtctgggc
     4381 gaagccctgg ccatggacag cgtggtggag aaggtctccg agctgttcgc cgagaagcac
     4441 cacagcctgt tcctcggccg tggcgcgcag tacccggtgg cgatggaagg tgcgctcaag
     4501 ctcaaggaaa tctcctatat ccacgccgaa gcctacccgg ccggcgagct caagcacggc
     4561 ccgttggcct tggtggacag cgatatgccg gtggtcaccg tggcgccgaa caacgagctg
     4621 ctggaaaagc tcaaatccaa cctgcaggaa gtgcgcgccc gtggcggcga gctggtggtg
     4681 tttgccgacg agcaggccgg catgagcaat ggcgagggca cccacgtggt gaacatgccg
     4741 catatccacg atgccctggc accgatcctc tacaccattc cgctgcagct gctgtcctac
     4801 tacgtcgctg tactcaaggg caccgacgtc gatcaaccgc gcaacctggc caagagcgtc
     4861 acggttgagt aacggagggc aagcaggtga agcgtgaaga cataaagagc aagcaggcgc
     4921 agggcgtgct ctttgatacc catatcatcg ccaacccggc caatacccgg gaatggatag
     4981 tgctgttgaa gaaaggcgcg ggcaccagct tctttctggt cgatggccag gagcaggtgg
     5041 agtccttcgc cgatctcaat gggctgatcg aagagctgcg cctacttggc atcaagggcg
     5101 cggaggtaca tctctaagcc ctgccgttca cccgaggtac atatgcctat tcgctatgcg
     5161 agaagcctga ccggacggac gcgcagtgtt gcagccaatc ggcatctggg ttttgccctg
     5221 gctttcgttg caggagcgat caatgccggc ggctttcttg ccgtgcagca atacacttcg
     5281 catatgaccg gcatcgtttc ggccatggcg gacaatattg ctctgggtgc ctatggccta
     5341 gtgtggagtg gtctcggcgg tgtgctgtca tttctgctgg gcgcagcctg ctcgacggtg
     5401 atgatcaatt actcgcggcg ccgccagctg cacagcgaat acgccttgcc gttgctactc
     5461 gaagctgcgc tgttgctgtg ttttggcttt cttggcgcgt cgctggcgca gattaccggc
     5521 ctgttcgtgc cgctgacggt gatgctgctg tgcttcatca tgggcctgca gaacgcgctg
     5581 attaccaaac tctccagggc ggaaatccgc accacccata tcaccggaat catcaccgac
     5641 atcggtatcg agctaggcaa gctgttctat
//
LOCUS       DASRUM010000248         3912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1957961, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000248 DASRUM010000000
VERSION     DASRUM010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1957961"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..476
                     /locus_tag="VFF22_16400"
     CDS             135..476
                     /locus_tag="VFF22_16400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZX18432.1"
                     /translation="MSNELVIEDIQLGDGKEVVRGALITTQYHGFLEDGSKFDSSYDR
                     GKAFQCVIGTGRVIKGWDQGLMGMKVGGKRKLFVPSHLGYGERQFGAHIKPHSNLIFE
                     IELLEVLTRDD"
     gene            complement(496..1101)
                     /locus_tag="VFF22_16405"
     CDS             complement(496..1101)
                     /locus_tag="VFF22_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX18433.1"
                     /translation="MSNTNFSASIRAPQYSHLHAAGHLKKHEFGLTRVLAHWRDEQQT
                     RRALKIAGNPRSVLDLPCGTGRFWPLLAEQPNRLIFAADDSTESLEDAEAGQSLEVLK
                     QVESFQSSAYSIDMEDNFVDSILCMDLLHQIADPAERLLILREFYRVTRDTLIVSLKL
                     DTYHLIEVQFAQAGFDILDHSDFLPGYALWRLYVLRKRTPA"
     gene            complement(1394..2047)
                     /locus_tag="VFF22_16410"
     CDS             complement(1394..2047)
                     /locus_tag="VFF22_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HZX18434.1"
                     /translation="MSLSRHLPSSFSPLHSLAFATCLLAGVSAQAEETASSNRWVSDS
                     LNTYVRSGPTDGYRIVGTLVSGQKVTLLQTSGDYSQVRGDNGSAVWIANRDLQDVPGQ
                     AERLPQLEQKVAELSAELEGINDTWKTRVQGMQETLDSRKTLIDELQTARSALDSELS
                     QANSELRALQAQLGEENQQVLMRYMAYGGGIAGGGLLAGLILPTMLRVRRKRNDQWV"
     gene            complement(2394..3911)
                     /locus_tag="VFF22_16415"
     CDS             complement(2394..3911)
                     /locus_tag="VFF22_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HZX18435.1"
                     /translation="MIKQDDLIQSVADALQFISYYHPVDFIQAMHEAYLREESPAARD
                     SMAQILINSRMCATGHRPICQDTGIVTVFIRVGMDVRWTGATMSVDDMINEGVRRAYN
                     LPENVLRASILADPAGARKNTKDNTPAVIHYSIVPGDKVEVDVAAKGGGSENKSKMAM
                     LNPSDSIVDWVLKTVPEMGAGWCPPGMLGIGIGGTAEKAAVMAKEVLMEHIDIHELKA
                     RGPQNKIEEMRLELFEKVNQLGIGAQGLGGLTTVLDVKIMDYPTHAASLPVCMIPNCA
                     DTRHAHFVLDGTGPAELEAPDLSAYPEIVWEAGPSARRVDLDTLTPEDVQSCKPGETV
                     LLNGKMLTGRDAAHKRMVDMLNKGEALPVDLKGRFIYYVGPVDPVREEVVGPAGPTTA
                     TRMDKFTRQILESTGLLGMIGKSERGPIAIEAIKDNKAVYLMAVGGAAYLVAQAIKKS
                     RVVAFAELGMEAIYEFDVKDMPVTVAVDTKGESVHITGPAIWQKKIHDSLAVEIK"
BASE COUNT          686 a         1079 c         1282 g          865 t
ORIGIN      
        1 ctggcgccga ttgccgccac cgaactggcg caactggaag cgcggctatc cgaactgctg
       61 gcggtgctgg agccggctgg cgagtgattt tctgcaaacc cggccgtgag caggcctgtt
      121 agacgaggtg agtgatgagc aacgaattgg tgatcgaaga tattcaactg ggtgacggca
      181 aggaagtggt cagaggtgcg ctgatcacca cccagtacca cggatttctc gaagatggca
      241 gcaagttcga ttcctcctat gatcgcggca aagccttcca gtgcgtgatc ggcaccggcc
      301 gggtgatcaa gggctgggac cagggcctga tgggcatgaa agtgggcggc aagcgcaaac
      361 tctttgtacc gtcgcacctg ggctatggtg aacgccagtt cggcgcccat atcaaaccgc
      421 attccaacct gatctttgag attgagctgc tggaagtgct gacgcgggat gattgacgag
      481 tgctgattgc gcggcttatg ccggagtacg tttgcgcaat acatagaggc gccagagggc
      541 gtagccgggc agaaagtcgc tgtggtcgag gatatcgaaa cccgcttgag cgaattgcac
      601 ctcgatgagg tggtaggtgt cgagcttgag cgagactatc agcgtgtcgc gggtaacacg
      661 atagaactcg cgcaggatca gtaggcgctc agccgggtca gcaatctggt gcagaaggtc
      721 catgcacagg atgctgtcga caaagttgtc ttccatgtcg attgaatagg ctgagctttg
      781 aaagctttca acctgtttga gcacttcaag ggactgcccg gcttcggcat cttccagtga
      841 ctcggttgag tcgtcagcgg caaagatcag tcgattgggt tgctcggcca gcagtggcca
      901 gaaacgtcct gtgccgcagg gtaggtctaa caccgagcgt ggattgcctg cgattttcag
      961 tgcgcggcgt gtttgttgct catcgcgcca gtgggcaagc acacgcgtta gtccgaactc
     1021 atgttttttc aagtggccgg ccgcatgcag gtgactgtat tggggggcgc gtattgaggc
     1081 gctgaagttt gtgttgctca tgtgccctgt ccttcttctg ctgtttcagc gcgccgtgtg
     1141 tctcacggct cgatcaatgc tgctgtcggc agtatgtcca gtgaagctta aggaaagatt
     1201 aattcaggca gatgaaggag ctttgcagtg gcgctgatac aggctgtggt tgattggctc
     1261 ttgatcctta ttaaattgcg acgacccacg gtcgcaggca tgtcgtttac ttcgaacttg
     1321 taggggcttc catgcccggt ttggttaagg tcagcgcgcg tttgctagtc cgggtggacc
     1381 tgtatcctat ttttcaaacc cactgatcat ttcgcttgcg ccgcacccgc agcatggtcg
     1441 gcaggatcag cccagcgagc agcccgccac cggcgatgcc gccgccgtag gccatatagc
     1501 gcatcaacac ttgctggttt tcctcgccca gttgcgcttg cagtgcgcgc agttcggagt
     1561 tggcctggct tagctcactg tccagggctg agcgcgcggt ttgcagttcg tcgatcaggg
     1621 ttttgcgcga gtcaagggtt tcctgcatgc cttgcacgcg ggttttccag gtgtcattga
     1681 tgccttccag ttcggcgctc agttcggcca ctttctgttc cagctgcggc aggcgctcgg
     1741 cctggccggg tacgtcctgc aggtcgcggt tggcaatcca taccgcgctg ccgttatcac
     1801 cacgcacctg gctgtaatcg ccggaggttt gcagcagggt gaccttttgc ccggagacca
     1861 gggtgccgac gatgcggtag ccgtcggttg ggccgctgcg tacataggta ttcaggctgt
     1921 cgctgaccca gcggttgctg ctggcagttt cttcggcttg ggcgctcaca cccgccagca
     1981 gacaggtagc gaaggcgagg ctgtgcaaag ggctaaaact ggatgggaga tggcgagata
     2041 gggacatggg gcaatcttca catggcgatt gaacgaaacc ctgtaggcgg gtgcgataaa
     2101 tgccggccag gcgcgttgcc ttgagcagtt aggcgctcgg gatggagtgg cgcttgcagc
     2161 tgctcgctgg gcaaatggcc atgcaatcca cccggcgggc cgacagcaga cagacacttg
     2221 gcgagcgcgg ggagttcact cagcggcggg ttgcgggatg gatggttggt atttatgcgc
     2281 agcttgccgg gtggttcaca gcccggctgg ccgctcaacc tgacgctgcg ttcgccagcg
     2341 ctaaaaacaa caaacccggc acgtggccgg gtttgttggg gttgcagcag ggcttacttg
     2401 atctcgaccg ccaggctgtc gtggatcttc ttctgccaga tcgcagggcc ggtgatatgc
     2461 accgactcgc ccttggtgtc gaccgccacg gtaaccggca tgtctttcac gtcgaactcg
     2521 tagatggctt ccatgccaag ttcggcgaag gccaccacgc gcgacttctt gatagcctgg
     2581 gcgaccaggt aggcggcacc gccgacggcc atcaggtaca cggccttgtt gtccttgatc
     2641 gcctcgatgg caatcgggcc gcgctcggac ttgccgatca tgcccagaag gccagtgctt
     2701 tcgaggatct gccgggtgaa cttgtccatc cgcgtggcgg tagtcgggcc ggctgggccg
     2761 accacttctt cgcgtaccgg atcgaccggg ccgacgtaat agataaagcg gcccttgagg
     2821 tccaccggca gcgcttcacc cttgttcagc atatcgacca tgcgcttgtg cgcggcatcg
     2881 cggccggtga gcatcttgcc gttgagcagc acggtctcgc ccggcttaca gctctgcacg
     2941 tcttccgggg tcagggtatc gagatcgacg cggcgcgcgc tcgggccggc ttcccagacg
     3001 atttccgggt aggcggacag gtccggcgct tccagttcgg ccgggccagt gccgtcgagc
     3061 acgaagtggg cgtgacgggt gtcggcgcag ttggggatca tgcacaccgg cagactggcg
     3121 gcgtgggtcg ggtaatccat gatcttcacg tcgagcacgg tggtcaggcc gccgaggccc
     3181 tgagcgccga tgcccagctg gttaactttt tcgaacagtt cgaggcgcat ttcctcgatc
     3241 ttgttctgtg ggccgcgggc tttcagctcg tggatgtcaa tgtgctccat cagcacttcc
     3301 ttggccatta ccgcagcctt ctcggcggtg ccgccgatgc cgatgccgag catgcccggc
     3361 gggcaccagc cagcgcccat ttccggcacg gtcttgagta cccagtcgac gatcgagtcg
     3421 gacgggttga gcatggccat cttcgatttg ttctcggagc cgccgccttt ggctgcgacg
     3481 tccacttcca ctttgtcgcc agggacgatg gagtagtgga ttacggctgg ggtgttgtct
     3541 ttggtgttct tacgcgcgcc ggctgggtcg gcgaggatcg aggcacgcag gacgttttcc
     3601 ggcaggttgt aagcgcggcg tacgccttcg ttgatcatgt cgtcgacgct catggtggcg
     3661 ccggtccagc gcacgtccat gccgacgcgg ataaatacgg tgacgatgcc ggtgtcctgg
     3721 cagatcggcc ggtgaccggt ggcgcacatg cgcgagttga tcagaatctg tgccatggag
     3781 tcgcgcgcgg cgggggactc ttcgcgcagg taggcctcat gcatggcctg gataaaatcc
     3841 acggggtggt aataggagat gaactgcagg gcgtcggcga cgctctggat caggtcgtct
     3901 tgcttgatca cg
//
LOCUS       DASRUM010000249         8396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1128164, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000249 DASRUM010000000
VERSION     DASRUM010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128164"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VFF22_16420"
     CDS             complement(<1..369)
                     /locus_tag="VFF22_16420"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HZX18436.1"
                     /translation="MITAPRLHALHHWPLPTCLLLAGLLLWLQPARELSAALISATYL
                     ALCLHYCWPQRRMAQTGELLVAYASQGGKAQRLAERSCAQLLASGMPARCIALNQLSR
                     SHLATLDRLLLVVSTYGDGEA"
     gene            complement(579..1442)
                     /locus_tag="VFF22_16425"
     CDS             complement(579..1442)
                     /locus_tag="VFF22_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HZX18437.1"
                     /translation="MHPLVKWTALTLAICLPLSAQAHRAWMLPSATVLSGEDPWVTVD
                     AAVSNDLFYFEHFSMRIEGIGDISTQPAGQRARPAPKLHVFAPDGSDVKPQNGAIGRY
                     RSTFDLHLTQKGTYKLAIANSGLFASWKEGQENKRWMGKAEDFAKQVPANAAELKVAQ
                     NSSRMEVFVTSGNPTTAVLKPTGVGLELAPITHPNDLFAGEAAEFSLLLDGKPAAGVE
                     VSVIPGGNRYRDELGEINTTSDANGKISITWPNAGMYWLEAKLSSKEGVSAPATERRA
                     SYSATLEVLAP"
     gene            complement(1474..1986)
                     /locus_tag="VFF22_16430"
     CDS             complement(1474..1986)
                     /locus_tag="VFF22_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HZX18438.1"
                     /translation="MPKRLLLPLCALLSAPALAAELQLKVEIPRLQVAEYHRPYVAIW
                     LEQPDQKHVANLAVWYDQKLKDKEGEKWLKDLRQWWRRSGRSLEMPVDGVSGATRAVG
                     KHSLSFNSADTPLKQLAPGEYRLVVEAAREVGGRELLRLPFTWPPQQAASHSAQGSSE
                     LGQINLQLKP"
     gene            complement(1998..2597)
                     /locus_tag="VFF22_16435"
     CDS             complement(1998..2597)
                     /locus_tag="VFF22_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017246392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HZX18439.1"
                     /translation="MNALPWLGTLRQWHWISSALCLAGMLLFAVTGITLNHAAQIEAK
                     PQVHNHQAQLPETLQAALQDKQPSQGLSLDLRQWLEAELAIRLDGRDAEWSDGELYIG
                     LPRPGGDAWLSLDIESGALEFESTDRGWIAYLNDLHKGRNTGTAWSWFIDAFAVLSVV
                     FCLSGLLLLQRYAGNRPSTWPMLGLGLVLPVLLALLFIH"
     gene            complement(2665..4899)
                     /locus_tag="VFF22_16440"
     CDS             complement(2665..4899)
                     /locus_tag="VFF22_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZX18440.1"
                     /translation="MSFACKRTVLASAITLASAVMASAHANEPIALQDVVVSASGFEQ
                     KITDAPASISVLSQEDLQQKRYSNLAQALEDVEGIDVRQGTGKTGGLNISIRGLPSEY
                     TLILIDGRRQNTAGNVTPNGFNETATSFMPPMSAIERIEVIRGPMSTLYGSDAMGGVV
                     NIITKKVAKAWGAAISLDHTFQENRDYGETSSTSLYASGPVIDNLLGLAVRGSLFDRG
                     ASDLTFDNDSTVSKRGASPVEGLNYTAGARLTLTPHANHDFSLDVEQGRQVYNNDDCQ
                     LGTLDGKSGGNAINGCTVDAPNTISGYDDELVFERDQLALSHTARLGFGTLDSSLTHN
                     TTETIGRTIPGTRGVAYTGYPDIVAGDDRELKSTDLIFDSKLVSPLGDSHIATIGGQY
                     WDAEVSDGIASEDFQQKSWALFVEDEWRLRDDLALTLGARYEDHEAFGGHVSPRAYLV
                     WNTTDNWTLKGGVSRGYKTPTLNQLHDGISGVTGQGRTITIGSPDLEPEITTNTEFGV
                     YFDNLAGFDANATLFHNKFKDKIDDGTPLANCNFTANPNQPGCVSLGSGFTQESFSQS
                     VNIGEAETQGLELAGGWQFAPAWKLAMNYTYTDSEQKSGINEGAPLTNTPEHMAHARL
                     SWTTTDRLTLWFKGEYRGERARFTDKYENLTAANQALQDQVGDLKAYEVFHLGGSYRA
                     SENLTLNATLYNAFDKDFTKGKTYTTNTGTTGWASSYIQATQSTTGTLEEGRRLWLSA
                     NLTF"
     gene            5301..7823
                     /locus_tag="VFF22_16445"
     CDS             5301..7823
                     /locus_tag="VFF22_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016713192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase flavoprotein subunit alpha"
                     /protein_id="HZX18441.1"
                     /translation="MLKKILFQLHWFFGITAGLVLALIGVTGAAYSFQDELLRAFNPE
                     VLQVEVRAGGVLSPDVLVPHIEAASQQRVVRLNVEADVERAGRVGFAPPAGERRGEVR
                     YFDPYTGELLGEPSGQGFFDLMVDLHRFLAMGEYGKQITAASTLALIFFCLSGLYLRW
                     PRQALNWRVWLSLDWAKRGRAFNWDLHAVAGTWCLAFYLCAALTGLYWSYDWYREGLT
                     ALLTSEPAGEQRNRGGRPAAGTVPEVNYPALWAGLQQAAGPQLSGYSLRLPAAAKQPV
                     TVFYMREDAPHERAFNSLSLDAQSGAVKMHKRYYQQAFGDQLLTSMYALHVGSYFGLP
                     GRILMFLASLSMPLFAITGWLLYLDRRRKKRAMLQARGALQPSAGQGEGWLIGFASQS
                     GFAEQLAWQTAGQLQAAGLAVSVQPLAKLNEQMLRQAHNALFVLSTFGDGEAPDSARG
                     FERKLLGQALDLPNLRYAMLALGDRQYQHFCGFALRVQDWLARQGARSLFDAVQVDAA
                     DTTALASWQRQLGELTGAQPLHQGETLYGLWTLTRRELLNPGSQGAPTYLLALRAEQP
                     TNWSAGDILEVPPRHSAATVQSWLQSHAVDGKQTVSLDGLTQSLQQALRGKQLPEQFT
                     HLVGLHAQALLDALVPLAAREYSIASLPSDGELELIVRQERHADGSLGLGSGWLTEGA
                     TLHGQLLARVRRNSAFHAPDDARPLILIGNGTGLAGLRSLLKARIVAGHTANWLLFGE
                     RNAAHDFYCQAQLQGWLASGDLAWLDLAFSRDQAAKVYVQDRLRESAEQLREWLAEGA
                     AIYVCGSLEGMAAGVDAVLHELLGEAAVSTLREEGRYRRDVY"
     gene            7872..>8396
                     /locus_tag="VFF22_16450"
     CDS             7872..>8396
                     /locus_tag="VFF22_16450"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HZX18442.1"
                     /translation="MKRILLNCDMGESFGVWSMGDDVHAMPLVDQANLACGFHASDPM
                     TMQRTVALAVQHGVSIGAHPAYPDLLGFGRRHIACSPEEVTALVLYQLGALDAFCRAA
                     GTQLAYVKPHGALYNDLVRDDALFNAVLEACASYRKGLPLMVLALADNSRELRLADAA
                     DVPLMFEAFADRAYL"
BASE COUNT         1364 a         2457 c         2868 g         1707 t
ORIGIN      
        1 cgcttcgcca tcgccatagg tgctgaccac cagtagcaga cggtcgaggg ttgccagatg
       61 ggagcggctc aactgattca gggcgatgca gcgcgccggc atgccgctgg cgagcaattg
      121 cgcgcagctt cgctcggcca aacgctgggc tttgccgcct tggctggcgt aggcgaccag
      181 cagttcgccg gtctgcgcca tgcggcgctg cggccagcaa tagtgcaggc acaacgcgag
      241 ataggtagcg ctgatcagcg cggcgctgag ttcgcgcgcc ggttgcagcc atagcagcag
      301 gccggccagt agtaggcagg tcggcagtgg ccagtggtgc agggcgtgaa gtcgcggggc
      361 ggtgatcaaa atggattggc cagaataggg gctgcaggga caaggctatg ccccagtacg
      421 gtattgcgtt tattggtagg gtgcgctgtg cgcatcagcg tcagctgccg attcttggtg
      481 cgcacggcgc accctacgcc gggctgctgt gcagcacatt tgctctgtac aaacacgcca
      541 tcagcgctgg ggtaatgacc agcgctgacg gcttggcatt aaggcgccag cacttccagg
      601 gtggcgctgt aggaggcgcg gcgctcggtg gctggtgcgc tgacgccttc cttgctgctc
      661 agcttggctt ccagccagta catgccggcg ttcggccagg tgatgctgat cttgccgttg
      721 gcatcgctgg tggtgttgat ctcgcccagc tcgtcgcggt agcggttgcc gccggggatc
      781 acgctgactt ccacgccggc ggccggcttg ccatcgagca gcaggctgaa ctcggcggct
      841 tcaccggcga acaggtcgtt ggggtgggtg atcggtgcca gctccaggcc gacgccagtg
      901 ggtttcagca cagcagtcgt cgggttgccg gaggtgacga agacttccat gcggctgctg
      961 ttctgcgcca ctttcagctc ggcggcgttg gccggcacct gcttggcgaa atcttcggct
     1021 ttgcccatcc agcgcttgtt ttcctggcct tctttccagc tggcgaacag gccgctgttg
     1081 gcgatggcca gtttgtaggt gcctttctgg gtcaggtgca aatcgaaggt actgcggtag
     1141 cggccgatgg caccgttctg cggcttcacg tcgctgccgt ccggggcgaa cacgtgcagc
     1201 ttcggggctg gacgggcgcg ctggccggcg ggctgcgtgc ttatgtcacc gatgccctcg
     1261 atacgcatgg agaaatgctc gaagtagaac aggtcattgg acacggccgc atccacggtc
     1321 acccatgggt cttcaccaga gagtacggtg gccgacggca gcatccaggc gcggtgggcc
     1381 tgggcggaaa gcggcaggca gatggccagg gtcagggcgg tccattttac gagtgggtgc
     1441 atggcagtct tccttaataa gtgggggctg aattcaaggc ttgagttgca ggttgatctg
     1501 acccagctcg ctgctgccct gggcgctgtg actggcggct tgctgtggtg gccaggtgaa
     1561 gggcaggcgc agcagttcgc ggccgccgac ttcgcgggcg gcttccacca ccaggcggta
     1621 ttcgccaggt gccagctgct tgagcggtgt atcggcactg ttgaagctca ggctgtgctt
     1681 gcccacggcg cgggttgcac cgctgacgcc atcgaccggc atctccaggc tacggccgct
     1741 gcgccgccac cattggcgca ggtctttgag ccatttctcg ccttccttgt ccttgagctt
     1801 ctggtcgtac cacacggcca aattggcgac gtgtttctgg tccggttgct ccagccagat
     1861 cgccacgtag gggcggtgat actcggccac ctgtaggcgc ggaatttcca ccttaagctg
     1921 caattcggcc gcgagagcgg gcgcactcaa cagggcgcac aggggcaaca gcaagcgttt
     1981 cggcataagg gatgtcctta gtgaataaac agcagagcca gcaaaacggg aagcaccagg
     2041 cccaggccga gcatcggcca ggtgctgggg cggttaccgg cgtagcgttg cagcaacagc
     2101 aagccgctga ggcagaacac cacgctcagt acggcgaagg catcgataaa ccaggaccag
     2161 gccgtaccgg tattacggcc cttgtgcagg tcattcaggt aggcgatcca gccgcggtcg
     2221 gtggactcga actccagcgc gccggactcg atatccaggc ttagccaggc atcgccgccg
     2281 gggcgcggca ggccgatata caactcgcca tccgaccatt cggcatcgcg accatccagg
     2341 cgaatcgcca gctcggcttc cagccactga cgcagatcca gcgacagccc ctggcttggc
     2401 tgtttgtctt gcagcgcggc ctgcagcgtt tcgggcagct gcgcctggtg gttatgcacc
     2461 tgcggtttgg cctcgatctg cgcggcatgg ttgagggtga tgccggtgac ggcaaacagc
     2521 agcatgccgg ccaggcacag cgccgagctg atccagtgcc attggcgcaa agtgccgagc
     2581 caggggagag cgttcatgga gttcgcgagg tcagggagga gggggagacg gtaaggcccg
     2641 ccatttggcg ggccctatgc cgcgttagaa cgtcaggttg gctgacagcc agaggcggcg
     2701 gccttcttcc agggtgccgg tggtggactg tgtggcctgg atatagctgg aagcccagcc
     2761 tgtggtgccg gtattggtgg tgtaggtctt gcctttggtg aagtccttgt cgaaggcgtt
     2821 gtacagggtg gcgttcagcg tcaggttctc agaggctctg tacgagccgc cgagatggaa
     2881 cacctcgtag gccttcaggt cgccgacctg gtcctgtagc gcctggttgg ccgcggtgag
     2941 gttttcgtac ttgtcggtga agcgcgcccg ctcgccgcga tactcgccct tgaaccacag
     3001 ggtcaggcgg tcggtggtgg tccagctcag gcgggcgtgg gccatatgct ctggggtgtt
     3061 ggtcagcggc gcgccttcat tgatgccgct cttctgctcg ctatcggtgt aggtgtaatt
     3121 catcgccagc ttccaggccg gggcgaactg ccagccgccc gccagttcca gaccctgggt
     3181 ttcggcttcg ccaatattga cgctctggga gaagctctcc tgggtgaagc cgctacccag
     3241 gctcacgcaa cctggctggt ttggattggc ggtgaagttg caattggcca acggcgtgcc
     3301 gtcgtcaatc ttgtctttga acttgttgtg gaacagcgtg gcgttggcat cgaagccggc
     3361 caggttgtcg aagtacacgc cgaattcggt gttggtggtg atttccggtt ccaggtctgg
     3421 gctgccgatg gtgatggtgc ggccctggcc ggtcacgccg ctgatgccgt catgcagctg
     3481 gttcagcgtt ggggtcttgt agccgcggct gacgccgcct ttcagggtcc agttgtcagt
     3541 ggtgttccac accagataag cgcgcgggct gacgtggccg ccaaaggctt cgtggtcttc
     3601 atagcgcgcg cccagggtca gggccaggtc gtcgcgcagg cgccattcgt cttcgacaaa
     3661 cagggcccag gatttctgct ggaaatcttc cgaagcgatg ccatcgctga cttcggcatc
     3721 ccagtactga ccgccgatgg tggcgatatg cgagtcgccc aaagggctga ccaatttgct
     3781 gtcgaagatc aggtcggtgg atttcagctc acggtcatca ccggcaacga tgtccgggta
     3841 gccggtgtag gccacgccac gggtgccggg aatggtgcgg ccgatggttt cggtggtgtt
     3901 gtgggtcagg ctgctgtcca gggtgccgaa gccgaggcgc gcggtgtggc tgagggcgag
     3961 ctgatcacgc tcaaacacca gctcgtcgtc atagccgctg atggtgttgg gtgcgtcgac
     4021 ggtgcagcca ttgatcgcgt tgccgccgct tttgccatcc agcgtgccga gctgacagtc
     4081 gtcgttgttg tagacctggc ggccttgctc gacgtccagg gagaaatcgt gatttgcatg
     4141 cggagtcagg gtcagtcgag ccccggcggt gtagttgagg ccctccaccg gggaggcgcc
     4201 gcgcttgctg acggtcgagt cgttgtcgaa ggtcaggtcg gaagcaccgc ggtcaaacag
     4261 gctgccgcgc acggccaggc cgagcaggtt gtcgatcacc gggccgctgg cgtacaggct
     4321 ggtgctgctg gtttcgccgt agtcgcggtt ttcctggaag gtgtgatcca ggctgatcgc
     4381 cgcgccccac gccttggcga ctttcttggt gatgatattg accacgccgc ccatggcatc
     4441 ggagccataa agggtcgaca tcggcccacg gatcacttcg atgcgctcaa tggccgacat
     4501 cggcggcata aagctggtgg cggtttcgtt gaagccgtta ggggtgacat tgcccgcggt
     4561 gttctgccgg cggccatcaa tcaggatcag ggtgtactcg ctgggcaggc cgcgaatact
     4621 gatattcagg ccgccggtct tgccggtgcc ctgacgcaca tcgatgcctt ccacatcttc
     4681 cagcgcctgg gccaggttgc tgtagcgctt ttgttgcaga tcttcctggc tcaaaacgct
     4741 gatgctggcc ggcgcgtcgg tgattttctg ctcaaaacca gaggcgctga ccaccacatc
     4801 ttgcagggca atgggctcat tggcgtgtgc gctggccata acggcgctgg cgagggtaat
     4861 ggcgctggcc agaacagtgc gtttgcaggc gaaagacatc gatgactcca gtggatgcta
     4921 tggagaagag aggtgcggat gataatgaat tgcaaatacg gttcaatcgt aagcttcccg
     4981 tagcattttc cagggacgac cagatgcact gaaagccagc gctggtgagg cctgtggcga
     5041 tatgtaaatt atgtaaggac gggcttaggg gcgcgcgtta gcgctgtgtt ttgaagggaa
     5101 gccagatggt tttgcgctgc cagcacttga gcgccgctgg tcgatttttg ctgctttgcg
     5161 ggcgcgcgct ggatgctaat gcaagtgatt cgtaattgtt gtattgtgcg cgcccctgcg
     5221 cggttactgc gcaattcctt atctaaacat ccgtttctag acaaccgcta gccctggccg
     5281 gcggttccag ggctggaaaa atgctgaaga aaattctctt ccaactgcac tggtttttcg
     5341 gcatcacggc cgggttggtg ctggcgctga taggcgtgac cggcgcggcg tattcgtttc
     5401 aggacgaatt gctgcgcgcg tttaaccccg aggtgttgca agttgaagtg cgcgccggcg
     5461 gcgtgctgtc gcctgacgtg ctggtgccgc atatcgaggc ggccagccag cagcgcgtgg
     5521 tgcgcctgaa tgtggaagcg gatgtcgagc gcgccggtcg ggtaggtttc gcaccgcctg
     5581 ccggcgagcg ccggggcgag gtgcgctact tcgatcccta cacgggcgag ctgttgggcg
     5641 agccgagtgg ccagggcttt ttcgacctga tggtggatct gcaccgcttt cttgccatgg
     5701 gcgagtatgg caagcagatc accgccgcca gcaccctggc gctgatcttt ttctgcctct
     5761 ctgggttata cctgcgctgg ccgcgtcagg ccttgaactg gcgcgtctgg ctaagcctgg
     5821 actgggccaa gcgcggccgc gcgttcaatt gggatctgca tgcagtggcc ggcacctggt
     5881 gcctggcgtt ctatctgtgc gcggcgctga ccgggctgta ttggtcctac gactggtacc
     5941 gcgaagggct gaccgcgctg ctcaccagcg agcctgctgg cgagcagcgc aatcgcggcg
     6001 gccggccagc tgccggaaca gtgccagagg tgaattaccc ggcgctctgg gctggcctgc
     6061 agcaagccgc cgggccgcag ctgagcggct acagcctgcg cttgccggcc gctgccaagc
     6121 agccggtgac ggtgttctat atgcgtgagg atgcgcccca tgagcgcgcc tttaactcgc
     6181 tcagcctgga tgcgcaaagt ggcgcggtga agatgcacaa gcgctattac cagcaggcgt
     6241 ttggtgatca gctgctgacc agcatgtatg ccctgcacgt gggcagttac ttcggcctgc
     6301 ccggacgcat cctgatgttt ctcgccagcc tgagcatgcc gctgttcgcc atcaccggct
     6361 ggttgctgta cctcgatcgg cgacgcaaga agcgcgcaat gctgcaggcg cgcggcgcgc
     6421 tgcagccgag cgcagggcaa ggcgagggct ggttgattgg ttttgccagc cagagcggct
     6481 ttgccgaaca gctggcctgg caaaccgctg gccagttgca ggccgcaggc ctggccgtca
     6541 gcgtgcagcc cctggccaaa ctcaatgaac agatgctgcg ccaggcgcac aacgcgctgt
     6601 tcgtgctcag cacctttggc gacggcgagg cgccggacag cgcgcgcggc tttgagcgca
     6661 agttgctcgg tcaggctctg gatctaccga atctgcgcta cgccatgctc gccctcggtg
     6721 accgccagta tcagcacttc tgcggttttg ccctgcgggt gcaggactgg ttggcgcggc
     6781 agggtgcgcg cagcctgttc gatgcagtac aggtagacgc cgccgatacc acggcgctgg
     6841 ccagctggca gcgtcagctc ggcgaattga ccggcgctca gccgctgcat cagggcgaaa
     6901 cgctttacgg tctgtggacc ctgacgcgcc gcgaactgct taatcccggc agccagggtg
     6961 cgccgaccta tctgctggca ttgcgtgccg aacagccgac taactggagt gccggcgata
     7021 ttctcgaagt gccgccgcgt cacagcgccg ccacggtgca gtcctggttg cagtcccatg
     7081 cagtggacgg caagcaaacg gttagcctcg atggcctgac gcagtcgctg cagcaggcgc
     7141 tgcgcggcaa acagctgccc gagcagttca cccatctggt cggcctgcat gcacaggctt
     7201 tgctggatgc gctggtgccg ttggctgcgc gcgaatattc catcgcttcc ttgcccagcg
     7261 acggcgaact ggaactgatc gtgcgtcagg agcggcatgc cgacggctcg ttggggctcg
     7321 gctccggctg gctgaccgaa ggcgcgacgc tgcatggcca gttgctggcg cgggtgcggc
     7381 gtaacagcgc cttccatgcg ccagacgatg cgcggccgct gatcctgatc ggcaatggca
     7441 ccggtctggc cgggctgcgt agcctgctca aggcgcggat cgtcgccggg cacaccgcta
     7501 actggctgct gttcggcgag cgcaatgctg cgcatgactt ctattgccag gcgcagctgc
     7561 agggctggct ggccagcggc gacctggcgt ggctggacct ggcgttctcc cgcgatcagg
     7621 ccgccaaggt ctatgtgcag gatcgcctgc gtgagtccgc cgagcagctg cgcgaatggt
     7681 tggccgaggg cgccgcaatc tacgtctgcg gcagcctcga gggtatggcc gccggcgttg
     7741 acgccgtgct gcacgaattg ctgggtgagg cggcggtcag cacgctgcgc gaagaagggc
     7801 gttaccgtcg cgacgtgtat tgagccggcc caggcttccc gccacggcaa tgcgccacta
     7861 agctgatggg catgaaaaga atcctgctga actgcgacat gggtgaaagc ttcggcgtgt
     7921 ggagcatggg cgatgatgtg catgccatgc cgctggttga tcaggccaat ctggcttgcg
     7981 gctttcatgc ttccgacccg atgaccatgc aacgcaccgt cgccctggcg gtgcagcatg
     8041 gggtgagcat tggtgcgcac ccggcctatc cggatttgct cggttttggc cggcggcata
     8101 tcgcctgctc gccggaggag gtcaccgcgc tggtgctgta tcagctcggc gcgctggatg
     8161 ccttctgccg cgcggccggc actcagttgg cctacgtcaa accgcatggc gcgctgtaca
     8221 acgacctggt gcgtgacgat gcgctgttca acgcagtgct tgaggcctgc gccagttacc
     8281 gcaagggctt gccgctgatg gtgctggcgc tggccgacaa cagccgtgag ctgcgcctgg
     8341 ccgatgccgc cgatgtgccg ttgatgttcg aggcctttgc cgaccgtgct tacctg
//
LOCUS       DASRUM010000250         5893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1313548, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000250 DASRUM010000000
VERSION     DASRUM010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313548"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..2194
                     /gene="spoT"
                     /locus_tag="VFF22_16455"
     CDS             86..2194
                     /gene="spoT"
                     /locus_tag="VFF22_16455"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HZX18443.1"
                     /translation="MPSIDALADRLSTYLDGDQVNLVRRAYFYAEQAHDGQRRRSGEA
                     YVTHPLAVASILADMHMDHQSLMAAMLHDVIEDTGIAKEALDAQFGESVAELVDGVSK
                     LTQMNFETKAEAQAENFQKMAMAMARDIRVILVKLADRLHNMRTLEVLSGEKRRRIAK
                     ETLEIYAPIANRLGMHSMRIEFEDLGFKAMHPMRSERIRTAVKRARGNRKEIVNKIEE
                     SITHCLAREGMEGEVMGREKHLYGIYQKMRGKRRAFNEIMDVYAFRIVVDKVDTCYRV
                     LGAVHNLYKPLPGRFKDYIAIPKANGYQSLHTTLFGMHGVPIEIQIRTREMEELANNG
                     IAAHWLYKSNEEDQPKGSHARARQWVKGVLEMQQRAGNSLEFIESVKIDLFPDEVYVF
                     TPKGRIMELPKGSTAVDFAYAVHTDVGNTCIACRINRRLAPLSQALESGSTVEIVTAP
                     GARPNPAWLSFVVTGKARTHIRHALKLQRRSESISLGERLLNKVLAGFDSRVDKLSPE
                     RVQAVLAEYHQDVIEDLLEDIGLGNRMAYVVARRLLSDSNEALPSSEGPLAIRGTEGL
                     VLSYAKCCTPIPGDPIVGHLSAGKGMVVHLDSCKNIGEIRHNPEKCIQLSWAKDVVGE
                     FNVELRIELEHQRGLIALLAGSVNAADGNIEKISMDERDGRISVVQLVVSVHDRVHLA
                     RVIKKLRALTGVIRITRARA"
     gene            2255..2635
                     /locus_tag="VFF22_16460"
     CDS             2255..2635
                     /locus_tag="VFF22_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZX18444.1"
                     /translation="MSKSVITSDKAPAAIGTYSQAIKAGNTVYMSGQIPLDPKTMELV
                     EGFEAQTVQVFENLKAVAEAAGGSFKDIVKLNIFLTDLSHFAKVNEVMGRYFEQPYPA
                     RAAIGVAALPRGSQVEMDAILVLE"
     gene            2684..3418
                     /locus_tag="VFF22_16465"
     CDS             2684..3418
                     /locus_tag="VFF22_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18445.1"
                     /translation="MRCLFVALLTGVLLSGCASTPQDPSGTWINQAAIEAAVESGNLR
                     EALLAYGPNLEWQLDNQRQLASFSNGFERGEGSFKRQTDGQLRVQFYGDFQETLSLNG
                     DELIQAQSDTWPEQRFVRAPAAPGPLAPAGSSFEQALYSAYLGGTWLIEEGLGEGGLV
                     LFQADGSLQGLPGAERYALCLAGDCAAMSGEFDNLWLQLGDQGQSWLFEREGEQLRVF
                     EALNRSQIDEMPSYYKGQQRWLLVKR"
     gene            complement(3433..4284)
                     /locus_tag="VFF22_16470"
     CDS             complement(3433..4284)
                     /locus_tag="VFF22_16470"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX18446.1"
                     /translation="MSKTLMIAGCGDVGSRLGQQLSAAGWTVYGLRRSVALLPQGIRP
                     VAGDLHADACPAAWPSEALDYLVYCAAATEHDEAGYRAAYVDGLRRVLDWLAQQGQRP
                     KRLLFVSSSGVYGQQLGEWVDESSPAEAASYSGRIMREAEQLALHSGLPASLVRLTGI
                     YGPGREWLLKQVRQGYRVVSEPPLYGNRIHVDDAAGLLAYLLQGDARGVVLDDCYIGV
                     DDQPAPLHEVVAWLREQLDVSHWSEQSTVRRSGSKRCSNARARALGWTPQYPSYREGY
                     QVILAQL"
     gene            complement(4315..5790)
                     /gene="nuoN"
                     /locus_tag="VFF22_16475"
     CDS             complement(4315..5790)
                     /gene="nuoN"
                     /locus_tag="VFF22_16475"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HZX18447.1"
                     /translation="MEFTTQHFIALLPLLITSATVVLVMLAIAWKRNHTLTFGLSVLG
                     LNLALLSLLPALGVTPIEVTPLLLVDKFACYYMALVLAATLACVTLTHAYLGGESGKG
                     YPGNREELYLLMLLSAAGGLVLVSAQHLAGLFIGLELLSVPTYGMIAYAFFNKRSLEA
                     GIKYMVLSAAGSAFLLFGMALLYAESGNLTFAGIGAKLAADGLPSMLAQLGVGMMLIG
                     LSFKLSLVPFHLWTPDVYEGAPAPVAAFLATASKVAVFAVLLRLYQMSPATAGGWLND
                     LLTLIAIASIIFGNLLALLQNNLKRLLGYSSIAHFGYLLVALIASKGLAVEAVGVYLA
                     TYVLTSLGAFGVITLMSTPYNGRDADALYEYRGLFWRRPYLTAVLTVMMLSLAGIPLT
                     AGFIGKFYVVAAGVQSQQWWLLGALVIGSAIGVFYYLRVMVTLFMVEPNLRRHDAPFN
                     WAQRAGGMMLLFVAVLAFFLGVYPQPLLELVQQASLVALLP"
BASE COUNT         1272 a         1915 c         1779 g          927 t
ORIGIN      
        1 ggaagaggct aacgagcctc tgtaagccta tgccgggtcg acgtcgtcag gcgcagggtc
       61 atctgaatcg gcaaggggaa cgcccatgcc gagcatagac gccctcgccg acagactttc
      121 gacctacctc gacggcgacc aggtcaatct ggttcgccgc gcttatttct acgccgaaca
      181 agcccacgac ggccaacgcc gccgcagcgg cgaagcttac gtcacccatc ccctagcggt
      241 ggcgagcatc ctggctgata tgcacatgga ccatcagagc ctgatggccg cgatgctgca
      301 tgacgtgatc gaagacaccg gtattgccaa ggaagctctg gacgcccagt ttggcgaatc
      361 cgtggcggaa ctggtcgacg gggtcagcaa actgacccag atgaacttcg aaaccaaggc
      421 tgaggcacag gccgaaaact tccagaaaat ggccatggcc atggcccgcg acatccgggt
      481 gattctggtc aagctggccg accgtctgca caacatgcgc accctggaag tgctgtccgg
      541 cgaaaagcgc cgacgcattg ccaaggaaac cctggaaatc tacgcaccga ttgccaaccg
      601 tctgggcatg cacagcatgc gcatcgagtt cgaagacctg ggcttcaagg ccatgcaccc
      661 gatgcgctcc gagcgcatcc gcacagcggt caagcgtgct cggggcaacc gcaaggaaat
      721 cgtcaacaag atcgaagagt cgatcaccca ctgcctggcc cgcgaaggca tggaaggcga
      781 ggtgatgggc cgcgagaagc atctttacgg catctaccag aagatgcgtg gcaagcgccg
      841 ggcgttcaac gaaatcatgg acgtctacgc cttccgcatc gtcgtcgaca aggtcgatac
      901 ctgctaccgc gtgctcggtg ccgtgcataa cctgtacaag cccctgcccg gccgcttcaa
      961 ggattacatc gcaatcccca aagcaaacgg ctaccagtcg ctgcacacca cgctgttcgg
     1021 catgcacggc gtaccgatcg agattcagat ccgcacccgt gagatggaag agctggccaa
     1081 caacggaatt gccgcgcact ggctgtacaa atccaacgag gaagaccagc ccaagggcag
     1141 ccacgcccgc gcgcggcagt gggtcaaggg cgtgctggag atgcagcaac gcgccggcaa
     1201 ctcgctggaa tttatcgaga gcgtgaagat cgacctgttc cccgacgagg tctacgtgtt
     1261 cacgcccaag ggccgcatca tggagctgcc gaaaggctcc acggcggttg acttcgctta
     1321 cgccgtgcac accgatgtcg gcaacacctg catcgcctgc cgcataaacc gccgcctggc
     1381 gccactgtcg caagcactgg aaagcggctc gacagtggaa atcgtcaccg cacccggcgc
     1441 ccggccaaac ccggcctggc tgagcttcgt ggtcaccggc aaggcccgca cacatattcg
     1501 tcacgccctg aaactgcagc gccgctcgga gtccatcagc cttggcgaac gcctgctgaa
     1561 caaggtgctg gccggtttcg acagccgcgt cgacaagctc tcgcccgagc gggtgcaggc
     1621 ggtactcgcc gagtaccatc aggacgtcat cgaagacctg ctggaagaca tcggcctggg
     1681 caaccgcatg gcctatgtgg tggcgcgtcg cctgctcagc gacagcaacg aagccctgcc
     1741 gagcagcgaa gggccgctgg cgattcgcgg gaccgagggc ctggtgctga gctacgccaa
     1801 atgctgcacg ccgattccag gcgatccgat tgtcggtcac ctgtccgccg gcaaaggcat
     1861 ggtggtgcac ctggacagct gcaagaacat cggcgagatc cgtcacaacc cggaaaaatg
     1921 catccagctg tcctgggcca aggacgtggt cggcgagttc aatgtcgaac tgcgcatcga
     1981 gctggaacac cagcgcggcc tgatcgccct gctggccggc agcgtcaacg ccgccgacgg
     2041 caatatcgag aaaatcagca tggacgagcg cgacggccgc atcagcgtcg tgcaactggt
     2101 ggtcagcgtg catgaccgcg tgcacctggc ccgagtgatc aaaaagctgc gcgccctgac
     2161 cggggtaatc cgcatcaccc gtgcgcgcgc ctgaatcctt tcccgatgag cgtgcctgcc
     2221 atgctcgtca ttccctgacc ttattggagt tcccatgagc aagagcgtta tcaccagcga
     2281 caaggcccca gccgcaatcg gcacctactc ccaggcgatc aaggcgggca acaccgtgta
     2341 catgtccggg caaatcccgc tggacccgaa aaccatggag ctggttgaag gcttcgaagc
     2401 tcagaccgtt caggtattcg aaaacctcaa agcagtggcc gaagccgctg gcggttcgtt
     2461 caaggacatc gtcaagctga acatcttcct caccgacctg tcgcatttcg ccaaggtcaa
     2521 tgaagtcatg ggccgttact tcgagcagcc ctacccggcc cgcgccgcca tcggcgtagc
     2581 cgccctgcca cgcggctcgc aggtggaaat ggacgctatt ctggttctcg aataagcccc
     2641 tcggcggggc cgctgccccg cccttccctc ttgaggacgc cgcatgcgtt gcctgtttgt
     2701 tgccctgctc accggcgtcc tgctcagcgg ttgcgccagc acaccgcaag acccaagcgg
     2761 cacctggatc aaccaggcag ccattgaagc tgccgtcgaa agcggcaacc tgcgcgaagc
     2821 cctgctggcc tacggcccca acctggaatg gcaactcgac aaccaacgtc agctggccag
     2881 cttcagcaat ggtttcgagc gcggcgaagg cagcttcaaa cgccagaccg atggccagct
     2941 gcgcgtgcag ttctacggtg actttcagga aaccctgagc ctcaacggcg atgagctgat
     3001 ccaggcgcag agcgatacct ggccggagca gcgctttgtc cgcgcacctg ccgcgccggg
     3061 cccgcttgcg ccagcgggaa gcagcttcga gcaggcgtta tacagcgcct acctgggcgg
     3121 cacctggttg attgaggaag gtctgggcga aggcggcctg gtgctgttcc aggccgatgg
     3181 cagcctgcaa ggcctgccag gcgccgagcg ttacgccctg tgtctggcag gtgactgcgc
     3241 ggccatgagc ggtgagttcg acaacctctg gctgcaactg ggcgaccagg ggcaaagctg
     3301 gctgttcgag cgtgaaggcg agcagttgcg cgtcttcgaa gcgctcaacc gctcacagat
     3361 cgacgaaatg cccagctact acaaaggcca gcagcgctgg ctgctggtca aacgctaggg
     3421 ccgaaccgaa ccctacaact gcgccagaat cacctggtaa ccctcgcggt agctcgggta
     3481 ctgcggcgtc caacccaggg cgcgggcgcg ggcgttgctg caacgcttgc tacctgagcg
     3541 gcgcaccgtc gattgctcgg accaatgact gacatccagc tgctcgcgca accaggccac
     3601 cacttcatgc agcggcgcag gctgatcatc gacgccgata tagcagtcat ccagcaccac
     3661 cccacgggca tcgccctgca gcagataggc cagtagccca gccgcatcat cgacatggat
     3721 acggttgccg tacaacggcg gctcgctgac tacccgataa ccctggcgca cctgcttgag
     3781 caaccactcg cggcctggac cgtagatacc ggtaaggcgt accaggctgg cgggcagccc
     3841 gctatgcaaa gccagttgct cggcctcccg catgatccgc ccagagtagc tagcggcttc
     3901 tgcgggagaa ctttcatcga cccattcgcc gagctgttgg ccatacacgc cgctgctgga
     3961 gacaaacagc aggcgcttag gccgttggcc ctgttgcgcc agccagtcga ggacacggcg
     4021 caggccgtcg acataggcgg cgcggtagcc ggcctcgtcg tgctcggtgg cggccgcgca
     4081 atacaccagg taatccagcg cctcgctcgg ccaggcggcc gggcaggcgt cggcatgcag
     4141 atcaccggca accgggcgaa taccttgcgg caacagcgcc acgctgcggc gcaagccgta
     4201 caccgtccag cccgcagcgc tcagctgctg acccagacgg ctgccgacat caccacagcc
     4261 ggcaatcatc agtgtcttac tcatcgttgc gtattcctta gggtctgttg cttatcaggg
     4321 caacagtgcc accaagcttg cctgttgcac cagctccagc aacggctgcg gatacacacc
     4381 gaggaaaaac gccagcacgg cgacaaacag cagcatcatg ccgccagcgc gctgcgccca
     4441 gttgaaaggc gcatcgtgac gacgcaggtt cggctcgacc ataaacaggg tgaccatcac
     4501 ccgcaggtaa tagaacacgc cgatggcgct gccgattacc agggcgccga gcagccacca
     4561 ctgctgcgat tgcacaccgg cggcgaccac gtagaacttg ccgataaacc cagccgtcag
     4621 cggaatgccg gccagtgaca gcatcatcac ggtgagcaca gcggtcagat acggacggcg
     4681 ccagaacagc ccgcggtatt cgtagagggc atcggcatca cggccgttgt aaggcgtcga
     4741 catcagagtg atcacaccga acgcacccaa gctggtcagc acataggtgg ccaggtacac
     4801 cccaaccgcc tccactgcta agcccttgct ggcgatcagc gccaccagca ggtagccgaa
     4861 gtgggcgatg gaggaataac cgagcagacg cttgaggttg ttctgcagca gcgccagcag
     4921 gttgccgaag atgattgagg cgatggcgat cagcgtcagc agatcattta gccagccgcc
     4981 cgctgtcgct ggcgacatct gatacagacg cagcagcacg gcaaataccg cgaccttgct
     5041 ggccgtggcg aggaaggctg ccaccggagc cggggcgcct tcgtaaacgt ccggggtcca
     5101 caagtgaaac ggcaccagcg acagtttgaa ggacaggcca atcagcatca tgcccacgcc
     5161 caactgcgcc agcatgctcg gcaggccatc ggcggccaat ttggcgccga taccggcgaa
     5221 ggtcaggttg cccgactcgg catacagcag cgccatgccg aacagcagaa acgccgaacc
     5281 ggcggcggac agcaccatgt acttgatgcc ggcttccagc gaacgcttgt tgaagaaggc
     5341 gtaggcgatc atgccgtagg tcggcaccga gagcagttcc agaccaataa acaggcccgc
     5401 cagatgctgc gcgctgacca acaccagacc gccagcggca gacaacagca tcagcaggta
     5461 cagctcttcg cggttgcccg ggtaaccctt gccggactcg ccacccagat aggcgtgagt
     5521 caacgtcaca caggccaggg tcgcggccag caccagggcc atgtagtagc aggcgaattt
     5581 atccaccagc agcagtgggg tgacctcgat gggcgttacg cccagggccg gcagcagcga
     5641 cagcagtgcc aggttgaggc ccagcaccga gaggccgaag gtcagcgtgt ggttgcgctt
     5701 ccaggcgatg gccagcatca ccagcaccac cgtggcactg gtgatcagca gcggcagcag
     5761 cgcgataaag tgttgagtcg tgaattccat acgctactta ccgggccgga acaggttgat
     5821 tgagggcact acccagccac tgctgcacgc cttgcatgct ggcggcggag gtgtcgagca
     5881 ccggctgcgg gta
//
LOCUS       DASRUM010000251       119850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_297880, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000251 DASRUM010000000
VERSION     DASRUM010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 119850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 119850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..119850
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297880"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..1948
                     /locus_tag="VFF22_16480"
     CDS             251..1948
                     /locus_tag="VFF22_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HZX18448.1"
                     /translation="MQTLRDFWLLARPFWSSTERRPAFLLLFGTVLMNLCLVGVNILY
                     NIWNLHFYNALQALDYDAFLIGGGHFILLQLGLATFTVAAFHFQQKLTVRWRRWSTEN
                     TLQLWLQRHRYLKLQLTSPETDNPDQRIADDINLFISMSLKLTLGLMTALVSLFSFLH
                     ILWQASSLVKLPIAGQEVIIPGLLVWAALLYSIIGTGGAFWLGRTLPRLNFIQQRREA
                     DFRFALMRLRENAEAVAQYRGENEEHARFSRRLGAALENFWALVKQQKVVLGYSTFYM
                     RSAMIIPMFIMAPQFFAGTFPLGRLTQLSAAFGEVHEAMAYLVRVFPELAEWKSVIDR
                     LTGFQKRLGSVTSEPGVQLEHRPGSFAVQDLSLWLPEGKSLLGGLNLSLQPGESLLIQ
                     APSGYGKSTLIRALTGIWSHASGKALYDLGSVLTLAQKPYLPLGNLRDMLWYPASPHP
                     EDDLALHRVMQQCNLGHLVEHLDKELDWSQTLSLGEQQRCAFVRVLLIQPNVLFLDES
                     SSALDDATEAHCYGLLKQELPRTIIISVGHRASLQRFHTRTLDLEEYKTLEQQEVYS"
     gene            complement(2061..2456)
                     /gene="pcaC"
                     /locus_tag="VFF22_16485"
     CDS             complement(2061..2456)
                     /gene="pcaC"
                     /locus_tag="VFF22_16485"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HZX18449.1"
                     /translation="MDEKQRYDAGMQVRRAVLGDAHVDRSLQHLTPFNEEFQEMITRH
                     AWGDIWTRPGLPLHTRSLITIAMLIGMNREGELRLHLKAAKNNGVTRDEIKEVLMQSA
                     IYCGIPAANATFHLAEEVWDEMGVESLQG"
     gene            complement(2502..3290)
                     /gene="pcaD"
                     /locus_tag="VFF22_16490"
     CDS             complement(2502..3290)
                     /gene="pcaD"
                     /locus_tag="VFF22_16490"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HZX18450.1"
                     /translation="MSVVQLADGELYYTVDGPQDAPVLVLSNSLGTDLHMWDAQMPAF
                     TEHFRVLRYDTRGHGRSLVTEGFYSIEQNGRDVLALLDVLDIESAAFCGLSMGGLIGQ
                     WLAINAPARIERLVLCNTAAKIGSPEVWNPRIETVLRDGQAAMLALRDASIARWFTAQ
                     FAQAQPAKVEPVVGMLAATSPQGYAANCAAVRDADFREQVAGIVAPTLIVCGTADGVT
                     TPEHGRFMQERIHAAELVELHAAHLSNVEASELFTRRVLDFLTA"
     gene            complement(3302..4663)
                     /locus_tag="VFF22_16495"
     CDS             complement(3302..4663)
                     /locus_tag="VFF22_16495"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HZX18451.1"
                     /translation="MHLLSNQLFDAYFSQAAMRAVFSDHGRVQGMLDFEAALARAQAS
                     CGLIPAEVVGPIATACQAERYDFAALAQAIVTAGNSAIPLVKALGKQVAAQSTNAERY
                     VHLGATSQDAMDSGLILQLRAAIALLEQDLAQLAAALAVQAERYADTPLAGRTWLQHA
                     TPVTLGMKLAGVLGAITRHRQRLAEIKPRLLVLQFGGASGSLAALGEQAWPVSEALAR
                     ELDLELPEQPWHTQRDRLVEFASLLGLIAGSLGKFGRDLSLLMQTEAGEVFEPAAPGK
                     GGSSTMPHKRNPVSAAVLISAATRAPGLVATMFSAMPQEHERSLGLWHAEWETLPELC
                     CLVAGALQQALLLVPGLEVDAARMRSNLDLTQGLVLAEAVSIALAQRIGRDAAHHLLE
                     QCCQQAVREGVHLRQVLGANAEVSAQLSGAELDRLLDPALYLGQARRWVERAVAEHQN
                     FSY"
     gene            complement(4840..6045)
                     /gene="pcaF"
                     /locus_tag="VFF22_16500"
     CDS             complement(4840..6045)
                     /gene="pcaF"
                     /locus_tag="VFF22_16500"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HZX18452.1"
                     /translation="MSREVFICDAIRTPIGRFGGGLAAVRADDLAAIPLKALLERNPQ
                     LDPAAIDEVFMGCANQAGEDNRNVARMALLLAGLPDSVPGVTLNRLCASGMDAVGSAF
                     RAIASGEMELALAGGVESMSRAPYVMGKADGAWSRNQKIEDTTIGWRFINPLMKAQYG
                     VDAMPQTADNVADEYQVSRVDQDAFALRSQQRAGRAQAEGFFAEEIVPVVIKGKKGDS
                     VIEADEHLRPDTTAEALAKLKPVNGEGKTVTAGNASGVNDGAVALILASREAVQKYGL
                     TARAKVLGMASAGVAPRVMGIGPVPAVRKLLGRLKMSIHDFDVIELNEAFAAQGLAVT
                     RELGLADDSTKVNPNGGAIALGHPLGASGARLVQTALHQLEKSGGRLGLATMCVGVGQ
                     GLALAIERV"
     gene            complement(6042..6824)
                     /locus_tag="VFF22_16505"
     CDS             complement(6042..6824)
                     /locus_tag="VFF22_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HZX18453.1"
                     /translation="MSAYSTNEMMTVAASRRLSNTDVTFVGIGLPSKAANLARLTHAP
                     DVTLIYESGPIGAKPTVLPLSIGDGELAETADTVVPTGEIFRYWLQGGRIDVGFLGAA
                     QVDKFGNINTTVIGDYHNPKVRLPGAGGAPEIAGSAKKVFIILKQGHRTFVDKLAFIT
                     SVGHGEGGEHRQQLGLPGAGPVAIITDLCIMEPEAGSKEFVVTSIHPGVSREQIVAAT
                     GWQIRFAEQVTETEAPQAFELEALRALEARTAAAHGQAGGEE"
     gene            complement(6824..7672)
                     /locus_tag="VFF22_16510"
     CDS             complement(6824..7672)
                     /locus_tag="VFF22_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HZX18454.1"
                     /translation="MAEILTLREAIQRFVHDGDSIALEGFTHLIPTAASHEIIRQGRK
                     DLTLIRMTPDLVYDLLIGAGCAKKLIFSWGGNPGVGSLHRLRDAVEKGWPQPLQISEH
                     SHAAMANAYVAGASGLPFAVLRGYQGSDLLKVNPDIKFIQCPFTGEQLAAVPSVRPDV
                     TVIHAQKADRKGNVLIQGILGVQKEAALAAKRCIVTVEEIVDELNAPMNACVLPGWAL
                     TAVCLVPGGAHPSYAHGYSERDNRFYQAWDPIARDRETFTAWIDTYIRGTADFAAYQA
                     KLQEAK"
     gene            complement(7831..9117)
                     /locus_tag="VFF22_16515"
     CDS             complement(7831..9117)
                     /locus_tag="VFF22_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015802 - basic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family porin"
                     /protein_id="HZX18455.1"
                     /translation="MNDNSITSLRLLPLAIAAALPSLSQAGEGGFIEDTSATLQARNY
                     YFSRDFSDIVGPSQQSRAEEWAQGFILNIKSGYTDGPVGFGVDAIGLLGIKLDSGRGR
                     VNTGLLPLQEDGEAADDYSRLGLTGKMRISRTELKVGELQPNLPVLAFSDIRLLPPSY
                     QGLNVTSSEIAGLTLQAGHLNSTSLRNEAGDDKMLAMLGHLPQRGAVSDAFNYAGGDY
                     TFNATRSTASAWYGQLEDIYNQRFLGLKHSQPLGTWTLGANLGYYDAQEDGSRLLGDI
                     DNQALFALLSAKHGGHTFYLGYQAMYGDSAFPRVFANITPLGNEVPTYEFASADERSW
                     QVRYDYDFSAIGVPGLVAGVRYIRGDNVDAQATNRGGPRYEGKDFERDLDIGYVVQSG
                     TLKGLGVRVRNVTARSNYRSDIDENRLIFNYTWNLL"
     gene            complement(9247..10464)
                     /locus_tag="VFF22_16520"
     CDS             complement(9247..10464)
                     /locus_tag="VFF22_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008037876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HZX18456.1"
                     /translation="MKALINDFSLSAVVAGFIATVISYAGPLVIIFQAAESAGMSHAV
                     LSSWVWAISIGSAVLGIVLSLRYRVPIIIAWSAPGSALLVSLLPQISLNEAVGAYMVA
                     SLIIFLVGISGAFDRIINRLPAAIAAGMLAGILFSFGTGLFVSLQTKPWLVLAMFASY
                     LLVKRVLPRYAVLAVLLVGCSIAFVTGDLRQEALVIGLATPVWISPEFSLSATLNIAL
                     PLVMVALTGQFVPGMAVLRNAGYPTPASPIIASSALGSLLLAPFACHGLNLAAITAAI
                     CTGREAHEDPARRYVAGVVGGLVYLLLGLFGATLVSLFSAFPKELVAALAGLALFGVI
                     AGSLASAMAQPDDREAALITFLVTASGMSFLGLSAAFWGLMFGIAAHLLLTRRATASA
                     AVPTAGSVGDPAP"
     gene            complement(10594..11535)
                     /gene="catA"
                     /locus_tag="VFF22_16525"
     CDS             complement(10594..11535)
                     /gene="catA"
                     /locus_tag="VFF22_16525"
                     /EC_number="1.13.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019615 - catechol catabolic process,
                     ortho-cleavage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 1,2-dioxygenase"
                     /protein_id="HZX18457.1"
                     /translation="MTVNVSHTAEVQKLFNEAAGLNNDQGSPRVKQIMLRLINDAAKL
                     IEDLEISDDEFWHAVDYLNRLGGRNEAGLLVPGLGLEHFLDLLQDAKDAQAGLTGGTP
                     RTIEGPLYVAGAPLAEGEVRMDDGSELDSATVMFLEGVVRDTQGTPVAGAVVDLWHAN
                     TQGNYSFFDKSQSEYNLRRRIVTDAEGRYRARSIVPSGYGCSPDGPTQECLDLLGRHG
                     QRPAHIHFFISAPGHRHLTTQINLAGEQYLWDDFAYATREGLVGDIKFVEDAAAARDC
                     GVQGSRFAELQFDFQLQKAPAAQAEQRSKRPRALQGA"
     gene            complement(11566..11856)
                     /gene="catC"
                     /locus_tag="VFF22_16530"
     CDS             complement(11566..11856)
                     /gene="catC"
                     /locus_tag="VFF22_16530"
                     /EC_number="5.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016159 - muconolactone delta-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase"
                     /protein_id="HZX18458.1"
                     /translation="MLFHVKMTVKLPADMQPAQAAKLKADEKELAQGLMREGKWRHLW
                     RIAGHYANYSVFDVASVEALHDTLLQLPLFPYMDIEINGVCRHPSSIHSDDR"
     gene            complement(11873..12994)
                     /locus_tag="VFF22_16535"
     CDS             complement(11873..12994)
                     /locus_tag="VFF22_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muconate cycloisomerase family protein"
                     /protein_id="HZX18459.1"
                     /translation="MSQVLIESVAATIVDLPTIRPHKLAMHTMQNQTLVLIRVRCSDA
                     IEGIGESTTIGGLAYGNESPESIKQNIDSHLAPLLIGQDAANINACMLRLDKAAKGNT
                     FAKSGLESALLDAQGKRLGLPVSELLGGRVRDSLEVAWTLASGDTARDIAEAEQMLGL
                     RRHRIFKLKIGANPVEQDLKHVIAIKRALGDSASVRVDVNQGWDESQAIRACQVLGDN
                     GIDLIEQPIARINRAGQMRLNQRSPAPIMADESIESVEDAFSLAADGAASIFALKIAK
                     NGGPRAVLRTAAIAEAAGIALYGGTMLEGAIGTLASAHAFVTLRQLTWGTELFGPLLL
                     TEDIVSEPPLYRDFALQVPRTPGLGLTLDEERLAFFRRP"
     gene            complement(13025..14365)
                     /locus_tag="VFF22_16540"
     CDS             complement(13025..14365)
                     /locus_tag="VFF22_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003301844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX18460.1"
                     /translation="MRQIDVHQVIDNARFSSFHWMVMALCALLLIFDGYDLFIYGVVL
                     PSIMQEWGLTPLQAGALGSYALFGMMFGALAFGTLADKIGRKKGIAICFALFSSATII
                     NGFASNPTEFGICRFLAGLGCGGLMPNAVALMNEYAPKRLRSTLVAVMFSGYSVGGML
                     AAGVGIYMLPRFGWESMFFAAAIPLLLLPLILWKLPESVGFLVRQGRHEQARAVLQKI
                     QPGLQIDAADQLQMSDTKGQGVAVFELFRDGRALRTLCLWLAFFCCLLMVYALSSWLP
                     KLMANAGYSLGSSLSFLLALNFGGMAGAILGGWLGDRFNLSKVMVAFFVAAAVSISLL
                     GFKSPTPLLYGLIFIAGATTIGTQILLYAGAAQFYGLSIRSTGLGWASGIGRNGAIVG
                     PLLGGALMGINLPLQLNFLAFAVPGAIAAIAMTVFAISAKRRQSASMALNAAKA"
     gene            complement(14511..15275)
                     /locus_tag="VFF22_16545"
     CDS             complement(14511..15275)
                     /locus_tag="VFF22_16545"
                     /EC_number="1.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate
                     dehydrogenase"
                     /protein_id="HZX18461.1"
                     /translation="MKMRFQEKVAVVTGAAQGIGRRVAERLLEEGALVLAVDRSELVF
                     ELAEHKAVLCLTADLERADDCQRVMQAAVERFGRLDILINNVGGTIWAKPFEHYVTEQ
                     IEAEVRRSLFPTLWCCHAALPQMLAQGSGAIVNVSSIATRGVNRVPYGAAKGGVNALT
                     ACLAFETAERGIRVNATAPGGTEAPPRRIPRNSAEPSADEQRWYQQIVDQTLASSLMK
                     RYGTIDEQVGAILFLASDEASYITGVTLPVGGGDLG"
     gene            complement(15387..16397)
                     /gene="benC"
                     /locus_tag="VFF22_16550"
     CDS             complement(15387..16397)
                     /gene="benC"
                     /locus_tag="VFF22_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020191856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate 1,2-dioxygenase electron transfer
                     component BenC"
                     /protein_id="HZX18462.1"
                     /translation="MSHKIALNFEDGVTRFIDAKLGETVADAAYRQGINIPLDCRDGA
                     CGACKCFAEAGQYDLGEEYIEDALSEAEAEQGYVLTCQMRAQSDCVVRVPASSEVCKT
                     QQASFEAAISAVRQLSDSTIALSIKGEALNQLAFLPGQYVNLKVPGSEQTRAYSFSSL
                     PKDGEVSFLIRNVPGGLMSSFLTGLAKAGDSMTLAGPLGSFYLREIKRPLLLLAGGTG
                     LAPFTAMLEKIAAEGSQHPLHLIYGVTNDFDLVEMDRLEALAASIPNFTYSACVANPD
                     SSYPHKGYVTQHIEPRHLNDGEVDIYLCGPPPMVEAVSGYIREQGISPANFYYEKFAA
                     SA"
     gene            complement(16490..16978)
                     /gene="benB"
                     /locus_tag="VFF22_16555"
     CDS             complement(16490..16978)
                     /gene="benB"
                     /locus_tag="VFF22_16555"
                     /EC_number="1.14.12.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate 1,2-dioxygenase small subunit"
                     /protein_id="HZX18463.1"
                     /translation="MSLSYDAVRDFLYREARYLDDKQWDEWLELYARDATFWMPAWDD
                     RDELVENPQTEISLIWYGNRSGLEDRVFRIRTERSSATIPDTRTSHNINNIELVEQVD
                     GLCQVRFNWHTLSFRYKTVDHFYGTSFYTLDCNGPNPLIKAKKVVLKNDYVRQIIDVY
                     HI"
     gene            complement(16979..18340)
                     /gene="benA"
                     /locus_tag="VFF22_16560"
     CDS             complement(16979..18340)
                     /gene="benA"
                     /locus_tag="VFF22_16560"
                     /EC_number="1.14.12.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate 1,2-dioxygenase large subunit"
                     /protein_id="HZX18464.1"
                     /translation="MNLGLDYLNGLLQEDKDQGIFRCKREMFTDPRLFDLEMQHIFEG
                     NWLYLAHDSQIPEKNDYYTTHMGRQPVFIARNKDGELNAFINACSHRGAMLCRFKSGN
                     KATYTCPFHGWTFNNSGKLLKVKDPKEAGYPDSFDCDGSHDLKKIARFESYRGFLFGS
                     LSDDVAPLEEFLGESRKIIDMICDQSAEGLEVLRGSSTYVYEGNWKLQAENGADGYHV
                     TAVHWNYAATQQQRKLKEAGDDIRAMSAGGWGKKGGGFYSFENGHLLLWTRWDNPEDR
                     PLFAERERLAGEFGEARADWMIGNSRNLCLYPNLYLMDQFGSQLRIARPLSVDKTEVT
                     IYCIAPKGECAEARARRIRQYEDFFNVSGMATPDDLEEFRACQQGFAGRAMEWNDMSR
                     GAKHWVDGADESAQEIDLHPLMSGVRTEDEGLFVLQHHYWQQQMIKALHKQQDQLIHV
                     EGA"
     gene            complement(18624..19580)
                     /locus_tag="VFF22_16565"
     CDS             complement(18624..19580)
                     /locus_tag="VFF22_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZX18465.1"
                     /translation="MDSCLLSAASCVFDRADPYAVSSYVNEHVGCHDIRLPTIGHPEA
                     SLNHRKFASLDLCRISYGGSVRVTSPALETIYHLQVLLRGHCLWRGHGEEHYFSPGEL
                     LLINPDDPVDLTYSDDCEKFIVKLPSTLLDKTCRDSQWQRPAEGIRFNSRHSLQQLEG
                     FTGLLQLVCSEAESSQTVQQVQEQYTRIIATKLLTLLDNNVSRMVPEQGAASFERILQ
                     FIEDNLKQDISVEQLAQLAHSSPRSLYALFERHTGTTPKSFIRQKKLEQIHCSLSDPA
                     SRIRNITEVALDYGFLHLGRFSESYKSAFGELPSETLRRRET"
     gene            complement(19823..20665)
                     /locus_tag="VFF22_16570"
     CDS             complement(19823..20665)
                     /locus_tag="VFF22_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZX18466.1"
                     /translation="MSEESRLPLSTLAPPIIASPAKRIEALTGDPNFMTSLARGLAVI
                     HAFQERKRHLTIAQISHRTEIPRAAVRRCLHTLIKLGYATTDGRTYSLLPKVLTLGHA
                     YLSSTPLAVTAQPILDRISEQLHEACSMATLEGDEILYIARSATPQRLISVDLSVGSR
                     LPAYCTSMGRILLAGLDDLALDDYLEHADLQVKTSRTIHTAEGLRANIAEIRQQGWVI
                     IDQELEIGLRSVAVPLKDSAGQVLAALNVGTHVSRVSRQELESRYLPVLLEASHELST
                     RLFH"
     gene            complement(20791..21732)
                     /locus_tag="VFF22_16575"
     CDS             complement(20791..21732)
                     /locus_tag="VFF22_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HZX18467.1"
                     /translation="MLQILGITAPIFILIGIGYLAARNGLVSPEQVTGMGRFVVTLAL
                     PALVIKALLERPLHEVFDRNYLLAYGLGSLAVFVLGFLFARLLRRDSTSGSAITALGM
                     SMSNSGFIGYPIVVLVVGEKAAVGLALGMLVENLLMLPLGLMLAEAGAQRGVGLKALA
                     LGTFKRLASNPIIIAISFGLLLALLDVRLPAVPLRVVEMLAQASAPVALFVIGASLVG
                     LKAGGLLGDVLQVSVGKLILHPLAVLLMFTWFPVQDPVLRMAGVLFACSPMMSIYPII
                     GQRFGLAGRCAAALLGATVLSFLTVSAFIGVLGGPLG"
     gene            complement(21743..22810)
                     /gene="zapE"
                     /locus_tag="VFF22_16580"
     CDS             complement(21743..22810)
                     /gene="zapE"
                     /locus_tag="VFF22_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HZX18468.1"
                     /translation="MSSRLTRADVRVRQHFEAVLQAHGYRADVAQQAAIDHLGQWLSA
                     FLQARPSWLRRRAAGVYLHGGVGRGKSFVLDACFAAAPLAQKRRVHFHVFLQELQQRL
                     GRYAGQADPLAKIAAEIAGQTRLLCFDEFHVHDIGDAVLLGRLLTLLVGQGVGLICTS
                     NYPPGGLCPNPLYRARFQPTIALLEQRFDVLQLDGGYDYRQGSTRQWGRLCWPLQEQD
                     DWLGAQLGLSIAATRDANLLVNQHPLRLRGHEGARAWLDFTELFRRPRSSADYLWLCA
                     EFRELAIGGVPRLAGEGVDVQQRFLNFIDIAYDRGIELLLGCAASLDELCPRGIQSDL
                     SRTHSRLQQLQALPLSAVSMN"
     gene            complement(22812..23420)
                     /gene="pcaG"
                     /locus_tag="VFF22_16585"
     CDS             complement(22812..23420)
                     /gene="pcaG"
                     /locus_tag="VFF22_16585"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HZX18469.1"
                     /translation="MPIQLLPETPSQTAGPYVHIGLALEAAGNPSRPQEIWSQMAKPD
                     APGEHITLIGNVYDGNGHLVRDSFLEFWQADHEGRYDSAFDLEKPFNGFGRTATTFDA
                     GSEWSVQTIKPGVVNNAAGVPMAPHINLSLFARGINIHLQTRLYFADEAEANAKDPVL
                     NLIEQPQRRETLLAYPCEVDGKPAYRFDIRIQGDGETVFFDF"
     gene            complement(23434..24153)
                     /gene="pcaH"
                     /locus_tag="VFF22_16590"
     CDS             complement(23434..24153)
                     /gene="pcaH"
                     /locus_tag="VFF22_16590"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010565183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HZX18470.1"
                     /translation="MSDAHNSRFVIRDRNWHPKAFTPDYKTSIARSPRQALVSIPQSI
                     SETSGPDFSHLKLGQHDNDLLLNFNNGGLPVGERIIVAGRVMDQYGKPIPHTLVEMWQ
                     ANAGGRYRHKNDRYLAPLDPNFGGVGRALTDSQGNYSFRTVKPGPYPWRNGPNDWRPA
                     HIHVSISGPSISTRLITQLYFEGDPLIPMCPIVKSIANPDAVQTLIARLDMANANPMD
                     CLAYRFDIVLRGQRQTHFENR"
     gene            complement(24257..25189)
                     /gene="pcaQ"
                     /locus_tag="VFF22_16595"
     CDS             complement(24257..25189)
                     /gene="pcaQ"
                     /locus_tag="VFF22_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pca operon transcription factor PcaQ"
                     /protein_id="HZX18471.1"
                     /translation="MNIDNRIKFRHLLCFLEVARQGSLARAAEVLAISQPAVSKTLKE
                     LEEVLAASLFDRSKQGVVPTAAGLAFLRYAGPSVQALREGVRSLRSGEHEAGVVRLGV
                     LSTVESSLLPEVVRRLHEQHAALVVSVITGPSAHLLDQLRVGALDLVVGRMTDSPQIE
                     GLTFEHLYSESMILVVRPAHPLLAADLRDRSQLDYYPVVLPLAGTTIRRYADSLYVQC
                     GIVMPQQRLETLSVALSRRYVQSSDAVWIAPQDAVRQDLARGELLELDIGLREPGGSV
                     GICSNPTAALSLAAQWCCEALREAAADYRESFNS"
     gene            25594..26034
                     /gene="aroQ"
                     /locus_tag="VFF22_16600"
     CDS             25594..26034
                     /gene="aroQ"
                     /locus_tag="VFF22_16600"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HZX18472.1"
                     /translation="MNHSLLVLNGPNLNMLGTREPATYGHETLADIGQLCQRAASQHD
                     LQLEFRQTNHEGELIDWIHQARGRFAGIVINPGAWTHTSVAIRDALVASELPVIEVHL
                     SNVHKREAFRHHSFVSGIAVGVLCGLGSNGYRLAIDHFAHLLKA"
     gene            26039..26899
                     /locus_tag="VFF22_16605"
     CDS             26039..26899
                     /locus_tag="VFF22_16605"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZX18473.1"
                     /translation="MTQTTQTSILAGLIGAGIQASRTPALHEHEGDAQGIRYLYRLID
                     LDALKLDISALPQLLKGAEQMNFTGLNITFPCKQAIIPLLDELSDEARGIGAVNTVVL
                     KDGKRIGHNTDCLGFAEGLRRGLGNVAKRRVVQMGAGGAGAAVAHALLSEGIEQLTIF
                     EVDPSRSQSLVDSLNAHFGNGRAVVGSDLAGAVAAADGLVNTTPVGMAKLPGIPLPAE
                     YLHAGLWVAEIIYFPLETELLKQARALGCRTLDGGTMAVFQAVKAFELFSGVAADAER
                     MQAHFHSMNG"
     gene            complement(26972..27643)
                     /locus_tag="VFF22_16610"
     CDS             complement(26972..27643)
                     /locus_tag="VFF22_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZX18474.1"
                     /translation="MTMTTELLEPTLETPRKSRKNNPEKTREDILRAAISEFVSHGLS
                     GARVDAIAERTKTSKRMIYYYFGSKEQLYVEVLEKLYGDIRRTEGLLQLAELEPREAL
                     QRLVEFTFDHHDRNVDFVRIVCIENIHNGENVKQSSAIRSMSHNVLQLLDETLRRGEA
                     SGAFRQGLQALDVHLLISSFCFYRVSNRHTFSHIFQIDLGDDAALKARHKQVICESVL
                     RYLQA"
     gene            complement(27758..29668)
                     /gene="quiC"
                     /locus_tag="VFF22_16615"
     CDS             complement(27758..29668)
                     /gene="quiC"
                     /locus_tag="VFF22_16615"
                     /EC_number="4.2.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046565 - 3-dehydroshikimate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019631 - quinate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019633 - shikimate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroshikimate dehydratase QuiC"
                     /protein_id="HZX18475.1"
                     /translation="MQRSIATVSLSGTLPEKLEAIAAAGFDGVEIFENDLLYYAGSPR
                     DVRKMCADLGLAISLFQPFRDFEGCRRDRLEKNLDRAERKFDLMQELGTDLVLVCSNV
                     AADSLGDEQILIDDLRLLGERAGARNLRIGYEALAWGRHVNTWQQVWNIVRQADHPAL
                     GVLLDSFHTLSLKGDPQGIAEIPGDKIFFVQMADAPILAMDVLEWSRHFRCFPGQGEF
                     DLAGFLAPILKSGYSGPLSLEIFNDGFRAAPPRANAADGLRSLLYLEEKTRQRLEKEA
                     ALPADPQLLFAPPPAGRYDGVEFLEFAVDEAQGAKLAGWLERLGFARSGTHRSKAVSL
                     LSQGDIKIVLNAEPYSFAHSFFEAHGPSLCATALRVKDSSLALERARAFKGQPYRGLV
                     GPNEREIPAVRAPDGSLIYLVESAEPGSTIYDSDFQLDSQAGAGGGLQRIDHMAMALP
                     ADSLDSWVLFYKSLLDFEADDEVVLPDPYGLVKSRAIRSRCSSVRLPLNVSENRNTAI
                     SHALSSYRGSGVHHIAFSCADIFAEVSRAKDAGVPLLDIPLNYYDDLAARFDFDDEFL
                     SELAYYNVLYDRDAQGGELFHVYTEAFDERFFFEIIQRKNGYIGYGAANVAVRLAAMA
                     KSRSGGAQQMRS"
     gene            complement(29944..31134)
                     /locus_tag="VFF22_16620"
     CDS             complement(29944..31134)
                     /locus_tag="VFF22_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009401179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015802 - basic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family porin"
                     /protein_id="HZX18476.1"
                     /translation="MALPSGAMAAGFTDDAKVTLGLRNFYINRNFVDPANSQSKAEEW
                     TQSMILDARSGFTQGTVGFGLDALAMGAIKLDGGRGTRNTGLLPVHDDGRPADNYGRL
                     ALAAKAKLSKTELKVGEWMAVLPILRSDDGRSLPQTFEGAQITSNEIAGLTLYGGQFR
                     QNSERNNASMEDMSYGGGLSDRFNFAGGEYKFNQDRTLVGLWNAELKDVYNQQYLQLL
                     HSQPVGDWTFGANLGYFQGSEDGSERAGELDNKTYSGLFSAKIGSNTFYVGLQKVSGD
                     TGWMRVNGTSGGTLANDSFNSSYDNAKERSWQVRHDYNFTAVGIPGLTLMNRYVSGDN
                     VHTANTNDGKEWGRESELAYTVQSGTFKNLNVKWRNASIRRDYSSTEFDENRLIFNYP
                     LSLL"
     gene            complement(31384..32403)
                     /locus_tag="VFF22_16625"
     CDS             complement(31384..32403)
                     /locus_tag="VFF22_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013693305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZX18477.1"
                     /translation="MQQLMKTLLAGACATGLLLSSAVSHSEEIRERTLRFAFQNVKEH
                     PQGQGAQKFADLLSEKSGGKLKVRLFPGGTLGGDVQTVSALQGGTLDITVLNSGILAA
                     QAADYAMLDFPFLFNSVEEAHAVIDGPVGEKLSAQLDSKGLVGLGYWDLGFRNLSNSK
                     HAVTKLEDMQGLKVRVIQSPIYLETFSALGANPVPMAFPEVYTALEQKTIDGQENPFT
                     VIEGNKFFEVQKYLSVTGHIFNPQSLIISQKTWSRLNDDEKGLIREAAAEAQKFQREV
                     TAASMDKSRAALEAEMAVNDISVEEKDRFRERVQPVIEKFSKDLDAGLVKMMYDEIAK
                     VRAAQ"
     gene            complement(32458..33738)
                     /locus_tag="VFF22_16630"
     CDS             complement(32458..33738)
                     /locus_tag="VFF22_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007944696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZX18478.1"
                     /translation="MTLVIFLGSLLGSMALGIPIAFALLLVSVALMLHLDLFDAQIVA
                     QNLLNGADSFPLMAVPFFMLAGEVMNAGGLSKRIVNIAMALVGHKRGGLGYVAIIASC
                     LLASLSGSAVADAAALAALLVPMMVLAGHNRARSAGLIAAGGIIAPIIPPSIGFIVFG
                     VASGVSISKLFLAGIVPGLMLGVALAIAWWFISRSENVETPPKRSRAEVLRALIEGSW
                     AMGLPLIIIFGLKFGIFTPTEAAVVAAVYSLFVSLVIYRELKVSQLYEVILSSAKTTS
                     VVMLLVAAAMVSSWLVTIADLPGQLAAILEPFMDNPTLLLVVIMLLIILVGTVMDMTP
                     TILILTPVLMPAITQAGIDPVYFGVLFLINTAIGLITPPVGTVLNVVSGVSKLKIEEI
                     VGGVWPFLLAQLTVLFLLVLFPQLVLGPLKFFTG"
     gene            complement(33735..34298)
                     /locus_tag="VFF22_16635"
     CDS             complement(33735..34298)
                     /locus_tag="VFF22_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020291306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HZX18479.1"
                     /translation="MTRIVDLYFKLLKLLIVLCMVAMIVLVFGNVVLRYAFNSGISVS
                     EELSRWFFVWMIFLGALVALKDRAHLGLDSLVKRLPPLGKRICLVIGHVLMLFICWLI
                     FKGSWQQAVINLNVVAPASGLSMALFYAAGLVFGASAAAIILYELYLALFGKLGDDEL
                     VMVKDNDAAEDIQPIQQEQNNSGSTRP"
     gene            complement(34865..35734)
                     /locus_tag="VFF22_16640"
     CDS             complement(34865..35734)
                     /locus_tag="VFF22_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005731535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX18480.1"
                     /translation="MHKASAAAVPVFKLYGETAAWPTPDLIHCESIPERSRLHDWEIK
                     PHRHGDLVQLLYVQGGSAELEVEGVVNRVQQPALQVVPALSVHGFRFSQDIQGHVLSL
                     ALPLVEQLGTVLDSPPLARAACYPAGEQRRYLDALFDSIAEEYNTQQPGRELMLQSLI
                     SALLVWVGRRSQALAYAETQVQDRGREHLQRFTRLLESHFREHRPIEQYASELGISAA
                     HLNALCRRLAGQSALQLINQRLLLEAKRCLVYTAMTINQVSDSLGFSEPAYFSRFFKR
                     GSGLSPKAFRLQR"
     gene            36031..37215
                     /gene="pobA"
                     /locus_tag="VFF22_16645"
     CDS             36031..37215
                     /gene="pobA"
                     /locus_tag="VFF22_16645"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HZX18481.1"
                     /translation="MKTQVAIIGAGPSGLLLGQLLLKAGIDNIIIERQSPDYVLGRIR
                     AGVLEQGMVDLLREAGVSERMDQEGLVHDGFELAFDGRRERIDLHALTGGKTVMIYGQ
                     TEVTRDLMAARQASGGITLYNVTDAQLHELKGEKPHVTFTHNNETVRLECEHIAGCDG
                     FHGVSRQSIPAGVLKEFERVYPFGWLGVLADTPPVNEELIYANHERGFALCSMRSPTR
                     TRYYVQVGSEEKVEDWSDKRFWDELKSRLPEETAARLITGPSIEKSIAPLRSYVVEPM
                     QYGHLFLVGDAAHIVPPTGAKGLNLAASDVSTLYRILLKVYREGRHDLLENYSTVCLK
                     RIWKAERFSWWMTSMLHKFPGTDAFSQRMQQTELDYFVGSEAGRKTIAENYVGLPYEA
                     IE"
     gene            38514..39233
                     /locus_tag="VFF22_16650"
     CDS             38514..39233
                     /locus_tag="VFF22_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18482.1"
                     /translation="MPVRLLFVLLLLPHLAFASPNAERLQAIRSQAFSTCSSLLVHYN
                     PNQNDSDPRHAERYRQTLQQLQRLVTLEKDPLLIQYANDMREHIEKLEHQSANTPELY
                     PTWINPLLEAQSRLDQQASVRYASATPIEPRRKTLHQLALDIERLQLLYQTRTFGSLA
                     VYVMPMDDTTFAQLDQQILQNFSALKQDWPEHAAELNKLLRKYDFIRPRLLKHELVWV
                     PGSAAYYLGQVTDGLARINAE"
     gene            39245..40705
                     /locus_tag="VFF22_16655"
     CDS             39245..40705
                     /locus_tag="VFF22_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015474992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HZX18483.1"
                     /translation="MIRTAASVPLTPTQLRRVLAGLMLGLFLAALDQTVVAVALADIA
                     SDLPGSQLLAWVVSAYLLAMTVSTPIFGKLGDLFGRRRLLAIAIGVFVLASLFCGLAQ
                     SMPQLVAARALQGIGAGGLLAIIQALIGDLIAPAERGRYQAWFSGMFALASLIGPTLG
                     GLLSSQWSWRWVFWINLPLGALAYILSQRRLAGLQENRRAARIDYLGSVLLIIGLCSL
                     LWLINRLGQQALWLNVQNGLLLLLAVVALNLFIIQQRRSLDPLMPLALLRIDAVRSGW
                     LLLFFASFQAVGLAVLIPLQAYNSGGTGVSASQLMALALGTPLGAFLGGHLSARLGRY
                     KPMIVAGAVLLPMALLVLSMLPSPHGPATLIVLLLCGAAIGLQFPTSLVAVQSAAPHH
                     HLGVVTGVCGLFRGLGGMLGVSLLTSLLWQLLPGISADSMAETGMNLSAVDSHLLREA
                     FATLLQIDAAITLLPLLIALRLKDRSLSRDLTAAPE"
     gene            complement(40710..40997)
                     /locus_tag="VFF22_16660"
     CDS             complement(40710..40997)
                     /locus_tag="VFF22_16660"
                     /inference="COORDINATES: protein motif:HMM:NF021496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2218 domain-containing protein"
                     /protein_id="HZX18484.1"
                     /translation="MTISEAYVTCVNPARLMTRLCKHWSHKFNVELGEEKGSVAFPNG
                     TCEFAVEPGVLQVNLHMPEENQVRMQQVVAEHLQRMAGTEALTVEWRARLE"
     gene            complement(41008..41844)
                     /locus_tag="VFF22_16665"
     CDS             complement(41008..41844)
                     /locus_tag="VFF22_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003184519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HZX18485.1"
                     /translation="MKRFYLGVTLLLALVGVSQAQDKSEGEQAKRLLQSAVEYYRLQG
                     DAALAAFSRQGEFIDGQHYVFVVDTQGTMLASGGSSAVLIGRDVSAVLEPELRTAFAQ
                     ALASAEDRVESAEYRWMNVRDGRVERKQVYYQRVGERIIAVGHYLPRATPQQAQSLLD
                     RAVSALEHDLQGTLGSINNLSADFREDDLYVFVVDLDNRRYVAHGYNLRLLGVDFASI
                     NDPQGKPVGAPILALMNKSYSAGYDYRWKNPVTGKVEDKYALLRKVGHYLVAVGYYRE
                     PQ"
     gene            complement(41889..42887)
                     /locus_tag="VFF22_16670"
     CDS             complement(41889..42887)
                     /locus_tag="VFF22_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HZX18486.1"
                     /translation="MVWRGGLLVGASLLLLGFAALGLRFYQQAQVALGSVQGCSVYRG
                     LPPGWPGDPLAGMQQIKGGEFTPGSYHGYADERPLGAVSVGDFWIDRTEVTQAQFAAF
                     VAATGYVTEAERDGGGALFATPSAEQLNATPLSWWRYVPGASWRQPDGPGDADATPGN
                     LPVALVTLADARAYAQWLGNQLPSEAEWEYAARGGVTGEPLNDTPLDDQGRPTANYWQ
                     GAFPLINTAEDGFAGRAPVGCFAANAYGLYDMIGNLWEWTADRYSGALQGHANGDPSA
                     LRLLPVAAEQHQVIKGGSFLCAANYCMRYRVAARERQEGDLATAHVGFRTIRRLGP"
     gene            complement(42887..44545)
                     /locus_tag="VFF22_16675"
     CDS             complement(42887..44545)
                     /locus_tag="VFF22_16675"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003288424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZX18487.1"
                     /translation="MHLNNVLPVVLLGLLAASNAVAADARPNILLIVADDLGYSDLGA
                     FGGEINTPNLDQLAAGGLQLTSMYAAPTCSPTRAMLMSGTDNHLVGLGTMGEALQPFQ
                     RGKPGYEGYLNQQAHSIAQLLKDGGYSTSMVGKWHLGLEPEQGPDRRGFQRSFSMLEG
                     GASHFKPATLNPTKVEQVHYRENGQAVELPEDFYSTDFYTDKLIAYLLDSQQADQPFF
                     AYAAYTSPHWPLHAPDAYLDKYRGRYDQGYEAVRQARIERMKKLGLITADFQSATPLP
                     ATPGLPTWEQLSPEQRKVEARKMEIYAAMVDNLDHNVGRLLDHLRQSGQLDNTLILFM
                     SDNGAAGEAHTQYYPAGPNTDNSYENLGRRGSQIDYGVRWAEVSAAPFRLFKGTTAEG
                     GISVPAIVQLPKSMQRQGLETGIARVDDLAPTFLALAAIADPGDSYAGQTKHPITGKS
                     MLPMLSGQASAHGQQGVLAGELFGNLYYREGNLKLLAMRPQGGFVDAPQPLRWQLFDL
                     ATDRGERNDLAASQADTLARLKQAWQGYARQVGVVVPPIPAQAQ"
     gene            complement(44564..45193)
                     /locus_tag="VFF22_16680"
     CDS             complement(44564..45193)
                     /locus_tag="VFF22_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HZX18488.1"
                     /translation="MAWTREQMAQRAAQELQDGFYVNLGIGLPTLVANYIPEGMDVWL
                     QSENGLLGIGPFPRTEDVDADLINAGKQTITTLPGSSFFNSADSFAMIRGGHINLSIL
                     GAMQVSAEGDLANWMIPGKMVKGMGGAMDLVAGVKRVVVLMEHCAKGGAAKILERCDL
                     PLTGVGVVDRIITDLAVLDVTEQGLQLVELAEGVSLSELRDATGCAVVA"
     gene            complement(45196..45891)
                     /locus_tag="VFF22_16685"
     CDS             complement(45196..45891)
                     /locus_tag="VFF22_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HZX18489.1"
                     /translation="MDKVFPSAAAALAGQVADGMTLAVGGFGLCGIPEALIAALRDSG
                     ARNLTAISNNAGVDGFGLGQLLETRQIRKMISSYVGENKEFERQYLAGELELEFTPQG
                     TLAEKLRAGGAGIPAFFTKTGYGTLVAEGKETRQFDGQWYVMEKSLTADVALVKAWKA
                     DKAGNLLFNKTARNFNPLAAMAGRVCIAEVEEIVEIGELDADQIHLPGIYVQRLVLNT
                     SPEKRIEQRTVRI"
     gene            complement(45895..46680)
                     /locus_tag="VFF22_16690"
     CDS             complement(45895..46680)
                     /locus_tag="VFF22_16690"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZX18490.1"
                     /translation="MQPSKRRLVDLSVTLDNNPYTDPPPLLPKIDYMDHQQGWPEMAA
                     MFPGLSKDQLPGDESWAAERLNITTHSGTHMDAPWHYASTTDGGKPAFGIDELPLEWC
                     LQPGVKLDFRHMPDGHVVTAAEVEAELARIGHALQPLDIVLVNTRAGALFGQPGYLEA
                     GIGMGREATLYLLERGVRVVGTDAWSWDAPFKYTRERFAASGDASIIWEGHKAGRDIG
                     YGQMEKLANLESLPANGFQVSCFPYKIKHASAGFVRAVAIFEE"
     gene            complement(46667..47665)
                     /locus_tag="VFF22_16695"
     CDS             complement(46667..47665)
                     /locus_tag="VFF22_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013692915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZX18491.1"
                     /translation="MRVMVTGANGFVGRALVQRLLDAGELRGQPISALMLLDKDLVGF
                     PEDNRLRRHHGSVTDAALLRRVLADGIDVVFHLVSIPGGAAEEQYALGYQVNLLASLE
                     LLDQLRSQPGAPVLVYASSVAVYGGALPARMDENAALRPELSYGAHKKMVETALNDLA
                     RRGEVDGRALRLPGIVARPREPNGLLSAFMSDLLQAFAAGEPYRCPVSAEASCWWMSL
                     RCCVDNLIHAAELDGSSLGPARVWQLPLLQLSIAQVLDALANAYGQERRALISFAPDA
                     GLQALFGSAPAMKTPQARALGFRHDGSAAALVRNSLNPLSAARRSRSAGVTAHATV"
     gene            complement(47676..48659)
                     /locus_tag="VFF22_16700"
     CDS             complement(47676..48659)
                     /locus_tag="VFF22_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZX18492.1"
                     /translation="MKLATLKDGSRDGRLLLVSRDLSLALDAGHIAATLQLAIENWWS
                     VEPELQALYRELNEGRAEGTFAFDPAEVMAPLPRAYQWLDGSAFLNHGELMQKAFHLD
                     PIDGAETTPLMYQGAGDDFLGGRDDIRLPSESQGIDFEGEFVVLVDEVPLGCPAEQAG
                     GHIKLILQINDVSLRALAPREMKTGFGFLQAKPSSSFAAVAVTPDELGDAWRDGRVHL
                     PLQVEWNGKWFGNPNGGAMNFSFPQLIAHAALTRRLGAGTLIGSGTVSNAERSVGSAC
                     IAERRAIEMIEHGAPQTGFMRFGDRVRMEVLDADGQSLFGGIDQRVVAAVG"
     gene            complement(48656..49237)
                     /locus_tag="VFF22_16705"
     CDS             complement(48656..49237)
                     /locus_tag="VFF22_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZX18493.1"
                     /translation="MQQLPGFKRVVTGHDAHGLAVVATSGATPNNFPLKAVPGTVFYE
                     VWNSKASPAVLDNGDDPTNQPLQLSPMPLGSVIRVVDIPPDSVQNQVSAADAVAAFAE
                     IGQAHAGTGQADSKHKLMHRTETLDYGVVTEGEVWLVLDSEDVHLKRGDIVVQRGTNH
                     AWSNRTEQMARMVFILLDGQYAADLAALHGVRS"
     gene            complement(49293..49883)
                     /locus_tag="VFF22_16710"
     CDS             complement(49293..49883)
                     /locus_tag="VFF22_16710"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003191526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HZX18494.1"
                     /translation="MNQSLPAAAIAQLFLEARSHNAWLEQSVPDELLKQLYDTLKQGP
                     TSNNCCPARFVFVRSAEAKQQLLPALKGHNGEKMLQAPVTAIIAFDSQFFEYLPELFP
                     IYDAGAPYRDNPQAAHTAALRNGSLQCAYLMLAARALGLDCGPMSGFDTALVDATFFA
                     DGRWKSNFLCSLGYGDVSKLRPSGPRLSFEQACLLV"
     gene            complement(49974..51182)
                     /locus_tag="VFF22_16715"
     CDS             complement(49974..51182)
                     /locus_tag="VFF22_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX18495.1"
                     /translation="MGAERQAGLPQSLLLLLGSCLPVLGAVLLAPVLPRMQEHFIDTP
                     GVAVLVPIALTLPALMIALLAPLAGVIADRLGRKPLLLGAMLLYAICGVLPLWLESLE
                     AIVISRAGIGLAEAAIMTCCTTLMGDYYNGARRERLFALQMVATSLSAAIFIAVGGML
                     GEHGWRTPFALYGIGLLFLPLMAWFLWEPRGQAAAASEVATCAFPWRALTPLYALSFL
                     AGLSLFVVPVQAGFLLNLLHVDAPQQIGMTMGASQLGVLAGALSFRLLSAMPGHRQMF
                     LAFALAGVGGALMAVADSHALVVVAVLINGLGIGLMMPTLITWIMSLVGFQQRGRAAG
                     FFTAAFFAGEFVSPLVVLAITGGVNSDLPTALGIIAALQLLVAVACLALPKRADQAPV
                     APATELPAQG"
     gene            complement(51353..53848)
                     /locus_tag="VFF22_16720"
     CDS             complement(51353..53848)
                     /locus_tag="VFF22_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZX18496.1"
                     /translation="MTSSTGSISSPADSLDNFDPRSGSVVERALFNHRLWVLLLCLAA
                     TLFLGFQSTRIELNASFEKMIPTNHPYIANYLDNQKELSGLGNALRIAVVNKQGDIYD
                     ATYLKTLQELSDKIYLLPGVDRAYMKSLWTPATRWVAVTEEGLDGGPVIPDDYDASPA
                     SIGALKRNVQQSNEIGQLVAFDQTSSIIYAPLLQKTPDGQALNYSTLSQQLESLRSQY
                     QSDSIEIRITGFAKKVGDLIEGLKQILLFFAVAILITTAVLFWYTRCVRSTMLVVFCS
                     LVAVVWQLGLLPLLGYELDPYSVLVPFLVFAIGMSHGAQKMNGIMQDIGRGMHRIVAA
                     RNTFRRLFIAGLTALLSDAVGFAVLMFIDIPVIQDLAVAASIGVAVLIITNLILLPIL
                     LSYLGVSPAAARRSLRDEQEEGGRMERHPLWRTLDLFTQKRGATICVLVAAGLAIGGY
                     LVAQNLKIGDLDAGAPELRADSRYNLDDAFMNRAYGASSDLFAIMVRTPSGVCASYDV
                     LRKVDALDWQLRTLPGVDSTNSLALLNRRMLVGLSEGNPKWYELQHNQAMLNMITASA
                     PRGLYNESCSLLTLYVFLTDHKAETLTGLVDHVAQFAAANNTDEVQFMLAAGNAGIEA
                     ATNIVVKQANREMLFWVYGAVILLCLITFRSWRATLCAIVPLILTSILCEALMVWLGI
                     GVKVATLPVIALGVGIGIDYALYVMSMLLVYQRQGASLSEAYYRALVSTGKVVILTGV
                     TLAIGVITWLFSPIKFQADMGVLLAFMFVWNMVGALLLLPALAYFLFPVRPKSASDEL
                     KDNAPRAAKEGAVTHLMLPIEAAGPQIDGTYGR"
     gene            complement(53853..54947)
                     /locus_tag="VFF22_16725"
     CDS             complement(53853..54947)
                     /locus_tag="VFF22_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003180914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HZX18497.1"
                     /translation="MNRISQAGYWFLALALPFSTAWCAADTGPVGLPLDTPAMQVAPT
                     QKTVLIDLARAGERLVAVGERGLVLLSDDNGQTWRQAVVPVSVSLSAVQFVDARNGWA
                     IGHAGVVLATRDGGESWVVQLDGLRAAQIELDAAKAEQVTALDQDSAEVRVQSAERLV
                     ADGADKPFLALEFVDAQQGLIVGAYGLALQTRDGGANWHSVVGHIANPMGMHLYAISR
                     QGSRWFLAGEQGYLARSDDDGASFAQLESPYEGSFFTLQSRADGALLVGGLKGHAFIT
                     QDAGASFARAPVPMPVSFSDATRLSTGQVLLANQAGMLFLSKGEALQPFSKPLGVPVS
                     SLVQAADGSLIVAGFTGLLRVPQPATVASE"
     gene            complement(55018..56382)
                     /locus_tag="VFF22_16730"
     CDS             complement(55018..56382)
                     /locus_tag="VFF22_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003200366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HZX18498.1"
                     /translation="MTTQFTLKALCLAVLAVVAPITLAASAEQASALGKTLTPFGAEK
                     AGNADGTIPAWDGGYTTVDPAYKPGGKRSDPFAADKPLFSITAQNMAQYADKLSDGTK
                     EMFKRYPDTYRLDIYQTRRTAAAPQWVYDNTLKNATRASLVDSSAGPVPQGAYGGIPF
                     PIPQNGAEAMWNHALNWRGTSLSMNFRHYLMTADGSQVMSTDGQAIQEMPYYYEDGTP
                     ETFEGDYWLFRLLNVGPPIRAGEQIMGRTNVNGDKSQAHVYLSGQRRVRKLPNACCDT
                     PTPATAGVMSFDELSVFQGRMDRFNWKLVGKKEVYIPYNTNKVQAAAKPEDLFGKHHL
                     NPDYVRWELHRVWVVEADLVPGKRHQLPKGRYYLDEDTWQAALGDRWDGNGQLAKTLW
                     SLSAVLPDLPAHASLSAGFYDLTSGAWFIQNLYTGKDSQYGAVDRYKASEFSPAAMAG
                     RGVR"
     gene            complement(56395..58074)
                     /locus_tag="VFF22_16735"
     CDS             complement(56395..58074)
                     /locus_tag="VFF22_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HZX18499.1"
                     /translation="MATRKIAASRRAPFTLTLAASLVLGANMAQAFQIDTGNPDLSLR
                     WDNTLKYSAAWRLQDPSDKLSESAVARNQDDGDRNFDKGLISNRLDILSELDVGYRDF
                     GARVSAAGWYDTVYQDDNANDNPARSNQRSVAFDQFTDDTRQLHGGDVELLDAFVYWN
                     GELADRALSVRAGRHGLIWGESLFFGANGIAGGMAPVDVVKAQSVPNTQFKEITRPVS
                     QLSTTYQLTDDLSLGAFYQFEWEETRLPGVGSYFSGSDTIGEGNERLVVGAPFPAFLG
                     GNPNSPAAFFHGKDKKAKSSGQGGLQLRYAAETAEYGLYAIQYHDKSPKLYLKPGSGA
                     PNFSTGQIGEFYWVYPEEIRALGASFATTIDEYSFAGEASMRWNMPLVSNGQTVLPGV
                     DADNDENPLYAVGRTAHVNLNVLASLGPSFISNEASLVGEIAWNRVLNVTKNRNALDP
                     NATDDGLGIKLVYSPTYRQLFPGVDVSVPVGVSYFPLGKSSAIGAFGPDNGGDMNIGV
                     TATYLDRVTLGLTYTHFYGAEDTTLNSANQFNFKQSLKDRDYLAFSIKTTF"
     gene            complement(58232..59356)
                     /locus_tag="VFF22_16740"
     CDS             complement(58232..59356)
                     /locus_tag="VFF22_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019751621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZX18500.1"
                     /translation="MNAVNKVLVVGGGIGGLCSAIALRRKGIEVDLVELKAEWTVYGV
                     GIIQQSNVVREMAKLGVLDGYLDAAFAFEDVSINTTDGQPLVRIPGQRLAGPEYPANV
                     GISRLALHDVLSETTVGLGASVRLGLSVETLSEDAEGVDVLFTDGSRDRYDLVVGADG
                     LFSKVRGLLFGDKYQPRFTGQSVWRYNFPRPAAIDHLANFQGPAGNAGLVPLADNLMY
                     MFVTSHEPGNPRMDSANLAAEMRKRLSGFGGLIGELREQITDSAQVVYKPLEVVFVSE
                     PWYRGRVLLIGDAAHAATPHLGQGAGMAIEDAVVLGEELTAGGSVTQQLERFMARRYE
                     RCKYIAESSILAGDKEIAHDHSFDRIGLVKQMLKLTAEPI"
     gene            complement(59500..59862)
                     /locus_tag="VFF22_16745"
     CDS             complement(59500..59862)
                     /locus_tag="VFF22_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZX18501.1"
                     /translation="MSHAAIALCRLDELVEGQARGFDPLHAGKDSLFALRHGGEVRVY
                     RNSCPHLDVRLEYRKDRFLSADGSRIVCYAHGAQFLPESGLCVYGPCLGEHLSALRWH
                     LDAGWLVLEAGQLALCAG"
     gene            complement(59877..60815)
                     /locus_tag="VFF22_16750"
     CDS             complement(59877..60815)
                     /locus_tag="VFF22_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZX18502.1"
                     /translation="MNIIGLDALIFGVDDIQACSDFLRDYGLQPVDVGSRGGRFEALD
                     GTAVIIRSADDASLPAAIGPAPSIRETVYGVACAADLDAIADELGRDRPVTRDANGVL
                     HSVDDMGFAIAFQVSVRRAFSAPADLTNAPGHAPQRPLNQPGITPDMSALPRTLSHVV
                     YFVPDAAKAEAFYQRLGFITTDTFIGAGPFMRPAGSDDHHCLFMIQTPPHMKGCEHFT
                     FHMGSGTEVLLAGRRFEEKGWTSFWGPGRHLFGSNWFWYFNSPLGCHVEYDADMDKHD
                     DEWEARVAPLNADNSQLFLFTSKAKWAPGGPPPKQA"
     gene            61125..62039
                     /locus_tag="VFF22_16755"
     CDS             61125..62039
                     /locus_tag="VFF22_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003173128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX18503.1"
                     /translation="MRFNHLDLNLLVALDVLLEEQNITRAAERLHMTQSATSGVLGRL
                     RSYFEDDLLVQVGRKMQPTPYALELVTPVREVLLTIQSSITAKPVFDPTSSKRHFRMV
                     TSDYLITVLLARVIQRIHQEAPHITFEMLAPSDNSGELLIRGEVDLMIVPERYILEGH
                     PSQLLFEEEHVCVVWDANPLVGDHLTLEQYMDMGHVSVGFGRTRHMSIEDWFMNQYGF
                     NRRIEVITNDFNTLPQLVVGTQRIATMHQRLARLYAAHLPLRILQPPVKIPVMREFML
                     WHRTMDRDPMHSWLRERISEFINDLEQH"
     gene            62230..63387
                     /locus_tag="VFF22_16760"
     CDS             62230..63387
                     /locus_tag="VFF22_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003180897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZX18504.1"
                     /translation="MFRYFPTNYVWDLSVNLAIEMGARIGEIEEMCKPLVEAAKQPDA
                     AGTAAFRETWAKMADKLCGLAEEDEAAGRLFSAGEKYSRASTYYLTCERLQAHGAPGR
                     EALYQRVLQTFARGIELSKENCERVEIPYEGAHISGLLVRAEGVTGPAPILLQVNGLD
                     STKEMKYRVGLPAWLAKRGVSSLIIDQPGTGEALRLHNLTARYDSEHWASRVVDWLET
                     RSDVDAKRIGMEGVSLGGYYCPRAVAFEPRFACGVVWGANHDWRDVQKRRLEKEGELP
                     VPHYWTHVRWVWGAKDMDEFMTIAENVHLDGVLDRIKVPFLVTHGSKDSQIPLKWAER
                     TYEQLVNSPKRELKIFTEREGGVQHSSFDNSINAGQYIADWVAENLGGRTA"
     gene            complement(63809..65737)
                     /locus_tag="VFF22_16765"
     CDS             complement(63809..65737)
                     /locus_tag="VFF22_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003200354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX18505.1"
                     /translation="MSALSRVLGNLSVGAKLSLGFGLVLLFTLGVALTAFHSLGVLQA
                     RGEQSRDEAATQVLILQARIAEKEFALNLAPSALIQVREVVEQLIRLLDASSSSAPHV
                     AMGDAASAYLQQFLGYADSLRQAREARLRMQTLAQTAGDSFTVLFLDQLDALNAQLEQ
                     DISPSSEQMVLLEQTAALRDKLAKLRDSELYYSLDGEERYRSDWEMSMSDLLSAMQAL
                     DLGDQDQTSLQGARDALGNYRKAFEQFVASRGQSARSNAAMNMQTERVSELLAEADQQ
                     QTQAIAQDNQDAQRQLGLITLSALALGIGASLLIRQLILQPLRQAVQLAQRVAAGDLS
                     QAQEGDTRRDELGQLLDTVARMLGSLRGLVGRIGGGVGQLNGTAASLVEMIERNSRGV
                     EQQRQETDMAATAMQQMSATAQDVARNAGEASAAVAQADDQAREGDELVRQAGGKIDR
                     LAVEMTGCAEAMQSLLSESAAIGGVLDVIKAVAEQTNLLALNAAIEAARAGEHGRGFA
                     VVADEVRGLARRTQSSTKEIEGLIGRLRGVAQQAAERLQGSHALTDETVILAAQASQA
                     LARITHAVSSIEQMNQQIAAAAEQQSVVAEQVSQSMTRVREVAEDSARESQQLQSSTA
                     ELQRVGGELNDAVGHFRT"
     gene            66249..67205
                     /locus_tag="VFF22_16770"
     CDS             66249..67205
                     /locus_tag="VFF22_16770"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX18506.1"
                     /translation="MLQLFTDLKLRWRVATTSEERSPWAAARPTPSDKPAISRIALSF
                     IDKALRAKTRAFAGFFKRLAALLMVIASCTHAAESPTALRFATVDNFPPFSYMENGNL
                     TGIDIDLVYEMARRINISVQIQTYPWARVMGSVKSGEFDGAFAAFETAERQAFCLYVG
                     VLHVEEFYLFVRKDNAFPYTQIPDLYGKRVGIDRGVYVGEAFERAVNDGRIALEEVND
                     MGMINIRKLNAGRIDAVIGDLGVMNYHLKLLGLEQRIVALQPIREPTPAYLVLSKASQ
                     LKNKVELQDRMRRALHDMWNDGTYHLIYNRYVYKVGGDPLAR"
     gene            complement(67409..68509)
                     /locus_tag="VFF22_16775"
     CDS             complement(67409..68509)
                     /locus_tag="VFF22_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008073724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HZX18507.1"
                     /translation="MNSVMRSMAAAIAVTLMSGSVFAEQRTVRIYNWLEYLPGEILKD
                     FEAETGIRPIYDVFDSIEMLESKLLTGNSGYDVVFPSTANLGKFIQAGTFEPLDRSKL
                     KNWSHLDEPFMADLASSGDPGNQYAIPYMWGTTLIGYNVDMVRKALGPDAKLDSWDLI
                     FKEDNIAKLSKCGVGFIDAANEVLPIALHYLGLPSHSTEASDYRQAQELMASIRPHIN
                     YFNSSRYGMDLANGDICVGIGWSGGVALATRLADAAGKGVKVDMILPKEGAPVWSDVM
                     AIAKGAVNRDEAHEFLNYMMRPDVIAKASNAIGYPNANKDATELVDAHIRDNPNMYVP
                     ADVMPSLFVLKALPQNVERMRMRTWNAIRTGM"
     gene            complement(68551..69876)
                     /locus_tag="VFF22_16780"
     CDS             complement(68551..69876)
                     /locus_tag="VFF22_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019408240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HZX18508.1"
                     /translation="MTRVPLLPSLLATAVLASQSAVAGYTFQEGDLSGEVNLTAGGAA
                     IFTRNVNFGAGRVDTRNGEATGNNADWQEVYVKPAVSFSYALNPDFDLLAKGSIVAAS
                     TFGDGDAGGFTRGTDNRVSAEEAYVGFRAGEWRFTAGRQDYMIGSGFIVMDGNLDFHD
                     DAAFWLGPRTAFRDSAVLGWSRDALSVQAFSLRTDDHLGDYRMNGVNLDYAVEGVATF
                     GAMAMKLKAEDRDSNIATPRDGMEVYNVRALNAKLPGVEDLTLNAEYAVQGGRDGGIE
                     YDAKAWYAGAEYAFSDLPLQPRLGYRYAYFSGDEDLADNKRESWDALSKGFVDWGTWL
                     VGDVVGNYLLNNSNQKVHTWSLKTQVQPTLTVGAMHHQFSLDEKNLFGAPVSDRRFAD
                     ESVIFVDWVATPQLFTSLSYNWVQPKAAAKQLLGDKTFSAVEMYFTYRY"
     gene            complement(69993..71168)
                     /locus_tag="VFF22_16785"
     CDS             complement(69993..71168)
                     /locus_tag="VFF22_16785"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007941359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZX18509.1"
                     /translation="MTGFPPAEAQRVTWHNWMSPPFNKWGFRHLARLRPSISVAAGNG
                     AASDLPEAAEDLDGFSFESVCGLTVRLDEHLVASHCDAFIVLHRGKVKYERYFNGQTA
                     NDRHTMFSVTKSLIGVLAEQLICDGVLNPELRADHYVPEFAGSAFADATVRQLLDMAV
                     GIAYTERYDDPASESSQFGYACGFQPAPAAYAQFASLYQYLPSLRKSGEHGGFFHYVT
                     ATTEALAWVMERAGGIACPELLARVWGQIGCQRDGYFMADPWGRGVAGAGFNATARDI
                     ARFGLLLANRGVHEGRAVLDPRAIDAIAQGSDPAIYAGNEEFSAWTPGASYRSQWYVF
                     NDHSPALMACGINGQYLFVDREAELVIVKQSSLPEAVSLFDPDNVRALRALAHHFRG"
     gene            71417..72037
                     /locus_tag="VFF22_16790"
     CDS             71417..72037
                     /locus_tag="VFF22_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019751932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZX18510.1"
                     /translation="MSGLRERQKVMRRQAISRAGVKLFERQGFQSTTIEQIAQEAGVS
                     APTVFKYFGSKHEILLEMLQAGDERAISDARAHIAEMDDPLDALCYLEKVLVDQALAM
                     MPPPIWRELLLHVLGGGQSGLPDSYKRMNDILRSEIAALLRDLQSAGKLRADLDVDLA
                     AFLLNDYSHLQLLRLTSNEPLDLETHRQQVRQTSGLILYGMAAAKP"
     gene            complement(72084..74084)
                     /locus_tag="VFF22_16795"
     CDS             complement(72084..74084)
                     /locus_tag="VFF22_16795"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX18511.1"
                     /translation="MRTLSRQAEQQASISLGASIDARVGESVRSQAASVQAFFADTLR
                     AGEQAATEVERVVLLVRGLPHEHVPMREHLTAQIRSLLDDNPGLLSSYLVFLPGALDG
                     RDAEFVDLTAKGSNERGRFAPVWFRRGPQTLEQVAAAEKTLADETPMSDGRPARTWFD
                     CPLQSAAPCLLSPYLDTASGQPVLMTSLTLPVRIDGVVVAVLGVDIQLDQLQVLAQRA
                     QQALPGQGGLVWLLSDAGLVAATTADTGLLGQPWQAPAKAVRADGLQDIHAEQSFQPI
                     PSAQPWHVRVQVPAEQAFEPVISLQQTLSEQLQRSIRFELILGVAVSLLGLALIAFAS
                     RAIVNPLQGMAAVLDELADGEGDLSRRINYPREDELGALGCGMNRFLEVLQPTIASIK
                     TSMQALSAAAVHSVAGGKANLAATERQRSDIDHVATAMQQMRLSAHEVARNTASAAAA
                     TDGILRTIEDGTDLIDRTTAIVARQGVDLLASNDQVKALSLKSERISVVLSLIRGIAE
                     QTNLLALNAAIEAARAGDQGRGFAVVADEVRGLAQRTQDSIQEIHGIIEALQGDMQSV
                     VRSMASNQQLVSEADQLFTELVESLYGVGSAVMEIAQMNGQIASAAEEQSAVAEEIST
                     SVDRIRAVSDELTRSAGVSAQQAQAIDEQAAAQVRLLSRFRT"
     gene            complement(74296..74796)
                     /locus_tag="VFF22_16800"
     CDS             complement(74296..74796)
                     /locus_tag="VFF22_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007158814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HZX18512.1"
                     /translation="MSDIAIPLLHPVMSITALIAEPEVLGRCVDGYRINYPIIGGHFS
                     GPHLRGEVLPGGADCFLLRQDGVGDLDARYQLRTQDGVLINIHNIGQLTMTERGRELD
                     AQGIWPTPASEYNCTCTPRFQVAEGPLGWLTQHAFIGLVHYPSASEVLIQCYSLGPIA
                     LRTEAV"
     gene            complement(74729..75934)
                     /locus_tag="VFF22_16805"
     CDS             complement(74729..75934)
                     /locus_tag="VFF22_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008073724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZX18513.1"
                     /translation="MLLAAVLLLTSLASTGAAGQPRTVRIYNWIEYLPQEVLDSFERE
                     TGIHPVYTQFDSVEAMEAKLLTGNSGYDVVFPGTYSLDKLVVAGALEPLKREWLANWR
                     HLDEPFMSHLEVAGDPGNRYAVPYLWGTTLIGYNVDKLRQALGPDADVASWSLIFDAQ
                     RLAKLADCGVAMLDAPSEVLPILLQHLGLDPNSRQRQDYKQAQQALARIRPSVRYFDS
                     TRYGHDLASGEICLALGWSGGFVLAQQLADAAGQGVRIEMVLPREGAPIWSDVMVVAR
                     KAPNREEAHAFINYILRPDVIARISNSIGYPNPNKDATELLKTAPRPNPVMFMPPAVR
                     QTLFTLEPVGAPIERVRARIWRETTKPPLSEAATSACTAGELECHRERYCDSFAASGD
                     EYHCVDRRA"
     gene            complement(76179..78137)
                     /locus_tag="VFF22_16810"
     CDS             complement(76179..78137)
                     /locus_tag="VFF22_16810"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX18514.1"
                     /translation="MLKPIARLLANCSVGQKLLLGFGLVSVLAVAAIAQGLYATASLL
                     EHGRQVSAMAAVNRLTLQIRNAEKRYALEPTVTAMQEVEALVHTLDSQLRELQAQATA
                     DALPVLVAMQQAIVGYQQLFEQFVGHQRLATEALQHMQEQAEQARLQFEMVELDMYDQ
                     VRGVLNSGQALEGDPLSLAEQASALQRKLLVTRNSEFLYVQTGQPAALERWRESTEEM
                     SNAMELLQGRVDENSEESLAAALAALVEYRQAFQRYRDSLTLSQQTALQMDAQAQSVL
                     QQVEQAAAERQVQMDERGRSVMLLQAVGALVIVTLALLASLTIRNLIVAPLRETLQVA
                     RRIAAGDLSLDMQSGVVRRDELGQLMAAMAEMTANLRRLVSRIGESVTGLGQSTATLG
                     GLSEQSNNGAQRQRVETEQVATAMQQMAATVQQVAQNTEQASQSALEADRQARNSDQI
                     VQQTREHIYRLAEDMQLSEQAVQSLQQQVLRIGTVVDVIKAVAEQTNLLALNAAIEAA
                     RAGEQGRGFAVVADEVRSLAKRTQVSTGEIETLIAGLQNLSLHSVAQIQACARSMQST
                     VGLSDQVGSALQGITGAVSVIEQMNQQIAASAEQQSIVAEEVSRNVMHLRDEAERFAD
                     ANQQMVVASDELAGLERELLAAVAQFRA"
     gene            complement(78229..78942)
                     /locus_tag="VFF22_16815"
     CDS             complement(78229..78942)
                     /locus_tag="VFF22_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18515.1"
                     /translation="MSLWLLASSTLWAAAPAQQYQQLRSQATLLAVNALLYFDADPRA
                     QPDARHLASVREAQQRLDALALQLNLPADMGDSLASLNAWLDDLQAVRRDEAPRYPQL
                     LIGLLDSRAQLDRQSDAAYQQVQADLQPVVRLLNRQSLDIASLLLHAQARSARVLGDH
                     SLAYSEVEFTARDQAIEEGFVQLIALLPEQADTLHKQRLAYRFVRQRLLDRDPGRVTG
                     SLERYLAGIVVALDKLAAR"
     gene            complement(78967..81375)
                     /locus_tag="VFF22_16820"
     CDS             complement(78967..81375)
                     /locus_tag="VFF22_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019408250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZX18516.1"
                     /translation="MHSLSGSLQDFDRASGNRVERLIFNHRPWVILLCLLATLVLGWQ
                     AASIGINASYEKTIPTKHPFVVNFLAERSELSGLGNALRIAVTAEQGDIFSAEYLDTL
                     AKLNDEIYLLPGVDKPYMKSLWTPTTRWTAVTEDGLDGGTVIPGSYDGTPESLDEVRT
                     NVARSGEIGQLVAANFKSSVIYVPLLEINPETGKTLDAGEFSRRLEALRDKYQNEQVR
                     IHITGFTKLIGDLIEGLLQVALFFVAAVAITVAVLFWYTRCVRSTFVVVLCSLVGVLW
                     QVGLLAAMGYELDPYSILVPFLVFAIGMSHGSQKMNGIMQDIGRGMLPWIAARLTFRR
                     LFLAGLTALISDAVGFAVLMVIEIQVIQDLAITASIGVAVLILTNLVLLPVLLSYTGV
                     SPKAAQRSLHAEQDDRAGVARHPLWRLLDRFTSRSWAIAAVLVGLLLAVFGNQVSSQL
                     QIGDLDPGAPELRADSRYNRDVQFMNDNYAASSDIFVVMIRTPEDQCTRYSTLSAVDQ
                     LAWRLEQLPGVESTNSMAALSKIATAGYNEGNFKWYELTPNDGALGAVQTRAPRELFN
                     QGCSLLSLYVYLADHKAETLSRVVATSEAFIAEQQLPEVKFMLAAGSAGIEAATNIVV
                     EQAMQEMLYWVYGVVILLCWVTFRSWRAVLVAILPLMLTSILAEALMVGLGMGVKVAT
                     LPVIALGVGIGIDYALYVLSVILARMRAGDSLSEAYYRALLFTGKVVVLTGITLAIAV
                     ATWVFSPIKFQADMGILLAFMFLMNMLGALILLPALAYFLMPQKLFVAKSGDVPAVQP
                     DGVS"
     gene            complement(81419..82432)
                     /locus_tag="VFF22_16825"
     CDS             complement(81419..82432)
                     /locus_tag="VFF22_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008012968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HZX18517.1"
                     /translation="MAQSAGVAKIPLLDQPALQSAKAPRSVLLALTRAGERLVAVGER
                     GIILLSDDSGANWRQAQVPASVSLTSVQFVDAEQGWAVGHLGVVLHSADGGETWNKQL
                     DGIQAARLALASAQQGDDARLLKAAEWLVADGPDKPFLDLYFSDRQHGYIVGAYGLIL
                     RTTDGGASWQAWMGQVENPEGLNLYGIRAQGGELFIAGERGLLLRSSDAGQSFQKLES
                     PYEGSFFGLLASRSGGLLAYGLRGNAWWSSDRGDSWQQLDTGVESALAAGLELSDGRL
                     LLASQSGELLLSGDGGLHFEHRRTHQGATVAAVAQAADGSLTSVGLGGLSARQEIQAS
                     ARP"
     gene            complement(82580..83941)
                     /locus_tag="VFF22_16830"
     CDS             complement(82580..83941)
                     /locus_tag="VFF22_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HZX18518.1"
                     /translation="MQSQLSLRLSVLTIALMGSCFAHAAVSPDQAARLNADLTPMGAE
                     RAANADGSIPEWTGGYTQVPAGYKSGDKRADPFAADKPLFSVSASNMAEYAEQLAEGT
                     QALLKKYPDYRLDVYPTHRSAAAPQWVYDNTLQNATRATLEEATEKVSGAYGGIPFPI
                     PQNGAEVLWNYRLSWQGADTFYVPFDTWLMTAGGKKLQATRARFWYQHPYYFKEGSLE
                     TYGGKHLIGKLLTEQPSSKAGEGLMTHWDMDPSARAAWQYLVGQRRVRRAPNVAYDTP
                     DFVTSGVGFFDEAFMIFGPTDRHDLKLVGKKELLVPYNNNRAAAASSDELVKPGFLNP
                     DLVRWEKHRVWVVEATLKAGKRHVVPKRTYYVDEDSWQVLLIDGYDAQGKLGRHNYSL
                     TLLAPEMPVLTANVMWGSYNLETGAYFLNASANDLKVQYQDYPRPSASFFTPDAMASD
                     SMR"
     gene            complement(83960..85606)
                     /locus_tag="VFF22_16835"
     CDS             complement(83960..85606)
                     /locus_tag="VFF22_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007941369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HZX18519.1"
                     /translation="MHNSRCYPGFKRSALALSVCLAGWASQTQAMQFDLGDSEWRLRW
                     DNTIKYSQAWRLQGADDQITNSPTAGGLYPNIQSQGDNNFSSGLVSNRLDLFSEMDLS
                     RENYGLRLSAAAWYDDIYNQNTDSSLQRDFLNDTRDLHGRDAEILDAFVFVRGNIGES
                     SQGTLRLGRHSLIYGESLFYGGNGIANAQGPNDIVKLLSVPGTQFKEILRPVNQVSGQ
                     LQINPQLSVGAYYQFEWERSALPGAGSYLSDNDGIGEGSGSWTQVFGNETVHASDIEA
                     KNSGQGGMQLRYKPEGTELELGLYAAKYHDKAPSGLYAYLDGAAFAATGLPILGSYRQ
                     VFAEDIKTLGVSFSTASGPFNFAGEASLRWDAPLVSNLQVVLPGVAADNSDDALYAKG
                     KTAHVNLSTIYLLSPNALWDGGSVLAELAWNRTLSVTDNRAALDSNTTRDATALRILA
                     EPAYFQVVDGLDLSVPVGLGVVLDGRSSAVSKSGFGNSHAGDWSVGLKATYLQRWDFG
                     VNYVNFFGKKGAGLREDGNFSYAQSLADRDFVSVYVKTTF"
     gene            complement(85713..86480)
                     /locus_tag="VFF22_16840"
     CDS             complement(85713..86480)
                     /locus_tag="VFF22_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017699819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl-alcohol dehydrogenase"
                     /protein_id="HZX18520.1"
                     /translation="MKLTNKTIVVTGVSSGIGAETARTLRAHGATVIGVDRNEPSLTL
                     DGFVQADLSHPDAIEAAVQQLPAQVDGLCNIAGVPGTADPQLVAQVNYLGLRHLCGAV
                     LPRIRAGGAIVNVASILGAEWPLRLDLHKALASTESFAHGQAWLAENPVPQASCYQYF
                     KEALIVWNYLQAQPWFMQTSVRMNCVAPGPVFTPILGDFVSMLGKERIDADAHRMKRP
                     AYADEVAAAIAFLCADESRWINGVNIPVDGGLASTYI"
     gene            86759..88597
                     /locus_tag="VFF22_16845"
     CDS             86759..88597
                     /locus_tag="VFF22_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020795186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZX18521.1"
                     /translation="MADKLISLAELSRAAHKPSARGASEQLPPGAAPTLQDLAESLLF
                     SPSDGRIWLNGARMLLMHSSGIGALRRELIESMGLARARGIMLRTGYHCGARDAALIR
                     ERFPEADILAVFAAGPIIHGLEGAVKVEPVHFEFDMTLGTYYGEFLWHHSSEDDEHIA
                     QYGIGTEPACWMQTGYATGYTSAMVGRLILYREVECRSTGANTCRLIGKPVEEWDDAE
                     EELADLNAEPFVGSSASAAAVKPSAKNADKGALPSNLQAEPTAAQDSEMVGISSAFNA
                     ACHMLRRVAPTQATVLFTGESGVGKEMFARMLHRISPRHDAPFVAINCAAIPENLMES
                     ELFGVERGAFTGATQSRAGRFERADGGTLFLDEIATLSLVAQGKLLRALQEGEVERVG
                     GSRTLKVDVRVVAATNVDLRAAAQRGEFREDLFFRLNVFPIHLPPLRERKEDIPLLMT
                     HFLHRFTQRHGRQLSGFTPRTADTLLAYDFPGNIRELQNLVERGVISAPDDGAIDLAH
                     LFTSGERLAHPMFSIGVRGQLAAAPHESPPTQQTSSTPPAQQPETLHALLGDKNLSQL
                     SLQEVEDAMIDHCMVEAKGNVSEAARRLGLTRAQLSYRLSRRPAGE"
     gene            88947..90029
                     /locus_tag="VFF22_16850"
     CDS             88947..90029
                     /locus_tag="VFF22_16850"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012271820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZX18522.1"
                     /translation="MFPKNTWYVACTPDEIAEKPLGRQICGERIAFYRGPQGKVAAVE
                     DFCPHRGAPLSLGFVEDGQLICGYHGLAMGANGKTAAMPGQRVRGFPCIRSYPVEERY
                     GFIWVWPGDAEQADPALIHHLEWADSPDWAYGGGLFHINCDYRLMIDNLMDLTHETYV
                     HASSIGQKEIDEVAPVTTVEGDNVVTSRHMQNIMAPPFWRMALRGNGLADDVPVDRWQ
                     ICRFTPPSHVLIEVGVAHAGKGGYDAAPADKAASIVVDFITPETETSIWYFWGMARSF
                     KPEDQELTDSIREGQGKIFSEDLQMLEQQQANLLAHPERQLLKLNIDAGGVQSRRILE
                     HLIAQERAPAATPELIVSTAGARSAP"
     gene            90026..90979
                     /locus_tag="VFF22_16855"
     CDS             90026..90979
                     /locus_tag="VFF22_16855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003256146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HZX18523.1"
                     /translation="MIEVSVVARNNQAQDICSFELARLDGAPLPAFTAGAHIDVHLPG
                     GLVRQYSLCNPPWENQSYLIGVLKDPASRGGSQAMHEQMQVGQTLLISEPRNLFPLSE
                     NAQRSLLFAGGIGITPILCMAERLYQLGAEFELHYCARSIERAAFVERLQTAPFAERV
                     HLHFDDGDAEQRLDAANLLANPNPGTHLYVCGPGGFMEHVLSTAKAQGWPDERAHREY
                     FAAAPIDHANDGSFAVQLNSSGQIIQIPADQSVAEALEASGILIPLSCEQGICGTCLT
                     RVLAGEPEHRDLFLTEAEQAQNDQFTPCCSRAKSPLLVLDL"
     gene            complement(91213..91944)
                     /locus_tag="VFF22_16860"
     CDS             complement(91213..91944)
                     /locus_tag="VFF22_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZX18524.1"
                     /translation="MSKPGQRVLIALRKMIASGELAAGERIAEIPTAELLEVSRTPVR
                     IAFRTLEQEGLLVKCAGRGYTVRAVSPADIAGAVEVRGVLEGLAARQAAERGINAEVR
                     EALQQCLEQGDRLFQKGHVLEEDLESYHDLNMRFHQLIIAASGNPAIAEALARNDHLP
                     FASVSSLAVDLHDMAREYRRFNFAHMQHHAVFDALVNGQGSRAEAIMREHANATLRYA
                     EIFGPTQGASEGVKVIQKGAAEERS"
     gene            complement(91999..92658)
                     /locus_tag="VFF22_16865"
     CDS             complement(91999..92658)
                     /locus_tag="VFF22_16865"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZX18525.1"
                     /translation="MSETTPQLPRRGRPPLRSRDEQMSLLLDAAATEIGSGNFANVTM
                     DAIARSAGISKKTLYQLVASKEELMSQLVARDLSTLELLLESGIDCAEGLLSELRMYL
                     YLWARLTLSPLALGIYLMAAQGRENAPGIARIWYQEGAERCLNLLRGWLGKAAGKGLL
                     AREDVEPAVELIDALLISQPLKLFTLGVQQSWSDEQIGERVEVALGMFSRCFMARGAL
                     G"
     gene            complement(92830..93765)
                     /locus_tag="VFF22_16870"
     CDS             complement(92830..93765)
                     /locus_tag="VFF22_16870"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX18526.1"
                     /translation="MPLLKNLKAKLLRAFAKRMKGKFVYDAAQYPLRKVTEEYVPTAW
                     GPARALFYWSNTESAQPLPVYLNLHGGGFVAGVPEHDDSYCRRLAHNLGCLVVNVDYV
                     LAPEHPFPAGLRQSFAVLEWLAEQAATLGIDPQRIAVGGHSAGGNLATGVANLARGHQ
                     RLRVVHQLIDYAALDLSQAPELKTSTLDKPLLGVGLMRFFNSCYLSDPAQARDPLVSP
                     LLLAVDELRGMPPATLITAEYDILRLEGEAYAEKLRQAGVTVTERMFAGCDHMFTHLG
                     PDDSAEQAWQLMEDGLREAFQLEAVAEVRGAGALA"
     gene            complement(93789..95231)
                     /locus_tag="VFF22_16875"
     CDS             complement(93789..95231)
                     /locus_tag="VFF22_16875"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020795190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HZX18527.1"
                     /translation="MSILGHQTAAVSAEQLAAALAAMKQAHLAEGPASLELRRDRLDR
                     AIALLLDNREEIVAAVSADFGNRSREQTLLSDIAGSVASLKHCREHLAEWMQSDAHQA
                     PFPGCEARVDYQPLGVVGVISPWNFPIVLAFGPLASIFAAGNRAMLKPSELTPCTSAL
                     LAELIARYFDANELSTVLGSAEVGALFSAQPFDHLIFTGGTAVGRLIMRAAADNLVPV
                     TLELGGKSPVLVSRSADLATVVQRVMTVKTFNAGQICLAPDYLLLPEELLGDFVAEAV
                     RFVSEMYPTLRSNPDYTSIINPRHFDRLRSYLADAHAKGAYLVEINPAQEDLSDRETR
                     KIAPTLILDTNEQMQVLREEIFGPLLPIKTYRDFSAAIDHVNQQPRPLAAYYFGEDAG
                     ERQQMLERTTSGAVVINDCMSHVLFEELPFGGVGHSGMGAYHGVYGFRTFSHAKAVVV
                     QSPIGESNLAMRAPYGEMIHGLLDQLLTAN"
     gene            95568..96059
                     /locus_tag="VFF22_16880"
     CDS             95568..96059
                     /locus_tag="VFF22_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019473961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZX18528.1"
                     /translation="MAKKSPPTPEVNSPATEPSDTLLSQLIGYALRRAQLKVAQNLVE
                     QLNPFDLRPAQFSALLIIEASPGLMQTDLASILAIEPPQVVTLLNKLEKLGLAVRVRC
                     KPDKRSYGIFLSKAGETLLKQLKEIAATSDREATAALDEGERQQLLQLLHKVYRQDGN
                     GTD"
     gene            complement(96252..97499)
                     /locus_tag="VFF22_16885"
     CDS             complement(96252..97499)
                     /locus_tag="VFF22_16885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015635945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZX18529.1"
                     /translation="MAGGLYSAFDDVLMLEAVRTPWCDYGGALGLVSPTDLGIKVGRE
                     VLRRAEIDPAAVDSVLAGCVAQASFDAYMLPRHIGLYSGVPQTVPALGVQRICGTGFE
                     LLRQAGEQIAAGRAELALCVAAESMSRNPIAAYTHRGGFRLGAGVEFKDFLWEALFDP
                     AADIDMIGTAENLARRYGVVREEADAYALRSFALALAAQNEGLFADEIVSVHSESFAL
                     DGYQPRGVVLPRGVESLTQDSHPRPTSLAALSRLRAIHAGGVQTAGNSCAVVDGAAAA
                     LVGSASAAAGRRPLARLLATAVVGVAPELMGIGPAPAIRLLLERSGLRLDDIARFEIN
                     EAQAAQVLAVQRELQLDPQRLNPQGGSIALGHPLAATGLRLTLSLARQLRAANQRYGI
                     AAACIGGGQGMAVLLENPAFERG"
     gene            complement(97502..99364)
                     /locus_tag="VFF22_16890"
     CDS             complement(97502..99364)
                     /locus_tag="VFF22_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HZX18530.1"
                     /translation="MNSQSSGRVEPVYRPVSIGKSAVQLREVGQALYMEALEPLQAYP
                     QRLLERLLRWAEERPEQTFIARRGADGQWQRISYAEMLGRVRSIAQGLLGFDLSVERP
                     LLILSGNDLEHLQVACAALYLGVPYCPVSPAYSLVAQDYTKLQHICMLLQPGLVFAAD
                     GQAFARAIEAVVLADTPLLLTAGEVAGRECHSFASLLGNEACDKADAAFAATGPDSIA
                     KFLFTSGSTKLPKAVITTQRMLCANQQMLLQTFPVFGEAPPVLVDWLPWNHTFGGSHN
                     VGIVLYNGGTLYIDDGKPTPQHFAETLGNLREISPTAYLTVPKGWEELVAALEVDAEL
                     RETFFAQIKLFFFAGAGLSQAVWDRLDAVAEAHCGERIRMMAGIGMTETSPSCTFTTG
                     PLSMAGYVGLPAPGCEVKLVPVDGKLEGRFRGPHVMPGYWRSPEQSAAAFDEEGFYCS
                     GDALKLADPEHPEWGLMFDGRIAEDFKLNTGVFVSVGPLRTRVIMAGAPYIQDVVVTA
                     PNRECIGLLVFPQLAACRELAGLSPKATAEQVLAAPQVRAWLSELLEQLNQQATGSAS
                     RLAWACLMSEPPSLDKGEVTDKGSLNQRVILSCRAERIDALYQGADPQRVTAER"
     gene            complement(99501..100949)
                     /locus_tag="VFF22_16895"
     CDS             complement(99501..100949)
                     /locus_tag="VFF22_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010432695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZX18531.1"
                     /translation="MFEVPLLIGGQSRAAADGRVFERCNPVTGEVVSQVAAATLEDAD
                     AAVAAAQAAFPAWAALAPNERRARLLKAAEMLEDRAPEFLAVAGETGAMANWYGFNVH
                     LAASMLREAAAMTTQIYGEVIPSDVPGSFAMALRQPCGVVLGIAPWNAPVILGTRALA
                     MPLACGNTLVLKASELSPAVHRLIGQVLQDAGLGDGVVNVITNAPEDAPAIVARLIAN
                     PAVRRVNFTGSTHVGRIIGELAARHLKPALLELGGKAPLLVLDDADLDTAVEAAAFGA
                     YFNQGQICMSTERLVVDAKVADAFVDKLASKAASLHAGDPSDSLSVLGSLIDVSAGQR
                     IKALVDDALAKGARLVTGGQLDGSIMQATLLDGVTRDMRLYHEESFGPVAVVLRGEGD
                     EELLRLANDSEFGLSAAIFSRDVSRALLLAQRVESGICHINGPTVHDEAQMPFGGVKA
                     SGYGSFGSKASIDFFTQLRWVTIQNGPRHYPI"
     gene            complement(101022..101852)
                     /locus_tag="VFF22_16900"
     CDS             complement(101022..101852)
                     /locus_tag="VFF22_16900"
                     /EC_number="4.1.2.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005756117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxycinnamoyl CoA hydratase/lyase"
                     /protein_id="HZX18532.1"
                     /translation="MSSYENRWQTVKVEVEEGIGWVILNRPEKRNAMSPTLNREMVEV
                     LETLEQDPDVGVLVLTGAGEAWTAGMDLKEYFREIDAGPEILQEKIRREASQWQWKLL
                     RMYTKPTIAMVNGWCFGGGFSPLVACDLAICADEATFGLSEINWGIPPGNLVSKAMAD
                     TVGHRQSLYYIMTGKTFDGQKAAQMGLVNESVPLAQLRETTIELARNLLEKNPVVMRA
                     AKIGFKRCRELTWEQNEDYLYAKLDQSRLLDTEGGREQGMKQFLDEKSIKPGLQAYKR
                     "
     gene            102746..102967
                     /locus_tag="VFF22_16905"
     CDS             102746..102967
                     /locus_tag="VFF22_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18533.1"
                     /translation="MLNTLIAKLTTSLLRPKFNPGYQTRVYPRLGEMVIVHDSLDFGP
                     IATADELKPIDDFWLNDEMPITGLLTHRR"
     gene            103245..104129
                     /gene="dapA"
                     /locus_tag="VFF22_16910"
     CDS             103245..104129
                     /gene="dapA"
                     /locus_tag="VFF22_16910"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZX18534.1"
                     /translation="MIRGSIVALVTPMSENGAIDYSALNRLVDFHLREGSHGLVAVGT
                     TGECSALDFKEHLNVIRHIVERVGGRLPVIAGTGSNSTREAIALTAAAADAGADACLL
                     VTPYYVRPTQEGLYQHFAAIAKAVPIPQILYNVPARTACDLLPNTVSQLAELDTIVGI
                     KEATGDLVRARALLPLAADGFALYSGDDASAAEFMLLGGQGNISVTANVAPRAMAKLC
                     EAALAGDAAQARALQRYLQPLHAALGVETNPIAVKWALQRMKLIGAGIRAPLTWLGHA
                     HEALLCQAMDDCLIPSQP"
     gene            104163..106247
                     /locus_tag="VFF22_16915"
     CDS             104163..106247
                     /locus_tag="VFF22_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014337964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX18535.1"
                     /translation="MSFERISIQWRISLLSGCSLLAVVAVVLGITLYQAHRNAELFKR
                     ESTRILDESAQARLEASAVAQKLRIEHFFRSMELYGDGIAGQVLQLRTQHAGGAGSAD
                     TLRRQLVALIHDALLASPKTLGIYLVMQPDALDGQDSDFIAQEPMSSNEKGRFALYWS
                     QSEPGKLVQATLTEENINANPTPAGAEPQNAWYVCPVQSGKPCVVEPYAVEVEGRSTL
                     MSSLSIPLIDNGRVIGVLGFDISLATLQRLTETLSAELYQGQNAVYILSATHLLAGHS
                     AQPDKLGNPFASAPTPDSVQLSQVLQPLADSRPWHLHIDMPNAQLHAPAQRLQTQMDN
                     GQQDALWQSLGLGLGVSLLGVLLIGLSARSISRPLQRVAEMLDELVEGDGDLTRRLPR
                     QRSDELGRLVTGFNRFLDHLQPMVGEIQRAALGTRETADRAAGVAEDINAGMHRQHQQ
                     VEQAAVALQQINASAQEVAGSSALAADAARAADQAGQQGVLSFAKTTETITQLDQRLE
                     DAMQDVQALAGSSEEIGQVLDVICTIAEQTSLLALNAAIEAARAGDQGRGFAVVADEV
                     RHLADNTQNSVGKIRLVVENLQQLSRDVVSGMHRSREQARNSVGLLDDTHTALHTIGE
                     AISVIDRMNRQIAEASQAQSAGLEDVAQRVDGIRSISATLTTQIEASSHIGRSLHGMS
                     IQQQRLVEHFRT"
     gene            106273..107349
                     /locus_tag="VFF22_16920"
     CDS             106273..107349
                     /locus_tag="VFF22_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZX18536.1"
                     /translation="MRRLLYPLLLVAGLQAQAQDVIRFYSWKDYFDPAVLKDFEAQTG
                     IRVDYQAYTTVQELNQALRDGEAYDLIVPSHFMLKQLIAEQRLTRLDVRRLPSYSQLD
                     PWLLSMLAGIPAANQYSVPYLWGSIGLAINTQTAQAIYADALPNSWALLFDEAHSAQL
                     SACGIGLPDAPEEVTSVLLNYQGRRLSASGNRQIARSVQTLTPLAPLLRALDNWEHID
                     ALAAGRLCLAMTWSGHALKAMHDNPRLTYRIPQEGSAIYIDTLAIPSNASQPELAYRL
                     IDFLIAPDNIVRNALATKFYAPLPSDATALQTLIEENPLQVLSPAQRRRSYLLESLLP
                     AQKQTLEQSWQQFKSAASKVQPTP"
     gene            complement(107365..108309)
                     /locus_tag="VFF22_16925"
     CDS             complement(107365..108309)
                     /locus_tag="VFF22_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003200808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX18537.1"
                     /translation="MFSEIRSFSDPQQHASSILGWQQVYDQQSCGSFSSELRQVVADH
                     FQIFQESLGKRTVQHGRSPKGRLCIALLMSTDRSPLVLGQKVGAHNVMVLRDGEEFLL
                     HSPEGLCFLGANVDLVRFAKLAALELSSNKIELLKSMPQLSLSEPVQQRVRQRIYPVF
                     ESLLQRTEPVNAAVEKMLEDTLLDVFLDVFSNASDEGRDRRGSSAVCAYLVKRSQELA
                     MEAGVDSPISIIDLCEQLRVSRRTLQNSFQVITGTRPVEYLRNLRLNAVRRRLISTPA
                     SQFKVGEVAAEMGFYHLSHFATHYRALFGEYPSETRRA"
     gene            complement(108383..109123)
                     /locus_tag="VFF22_16930"
     CDS             complement(108383..109123)
                     /locus_tag="VFF22_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HZX18538.1"
                     /translation="MSAPSDLPAALRQAQRDAQPLVKLEQALSLEQGYDLQRQALMES
                     GQVLGGWKVALSGAPAQARLGLREPVYGWLSVDMARSPGSVLPLAQLIAPKLEVEVAF
                     ELGHDIAPGLHEDEALLAAVTRMAPAFEIADCRWQGWRFDVGAFLADDAAAGLYCLGP
                     WQAFDAQACRQIGCRLLHEDAVLGSTEAGEAPEQNLLWLLRRLLADDQPLRAGQVILS
                     GALLAPVDIQQGEYRLLMMGVELVVRFE"
     gene            complement(109120..110724)
                     /locus_tag="VFF22_16935"
     CDS             complement(109120..110724)
                     /locus_tag="VFF22_16935"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018927860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HZX18539.1"
                     /translation="MKRETTQVVIVGGGPNGITAAHYMGLYGIDCVVLELAQGILPYP
                     RAVGMDDEALRVLQTVGVAERAHRDMIANVPLRYYNARGVCFAEVKPSVATYGWPMRN
                     IFMQQLLEGTLREELVRHEGVDLREGHEMLSLEQDDDGVTLQVRDADGEAYELRARYV
                     IGADGGRSCVRKQLGISLIGLTHPRKWVVVDATNDTLDAPYTALHADPQRPFVCIYLP
                     YSQRRWEFMLLEGESEEAMCEEGNIRRMIRGHIGEAVDQLEIVRIRAYTHNSRVAERF
                     VSGRVALIGDAAHISPPWAGQGLNSGLRDVANLCWKLAAVLKGRANPAIIQTYDQERR
                     GHATDLIALADNMGAMLGMTNPLVAGVRDWLFQAVNTVDNLRSHLSEFKFKPRATLAK
                     GLVHHERQDVREDDPVGQLFIQPDVEDASGERIRLDQVLGPWYAVLAYRCNPLEQLSE
                     ETQAFWAGLETRFIQVNRSRSGSRNQPLSTAGVISVEDVDNCLAEWFSKARDNIVVVR
                     PDRFVAALTTPERLNDVLGKLARQLS"
     gene            complement(110743..111591)
                     /locus_tag="VFF22_16940"
     CDS             complement(110743..111591)
                     /locus_tag="VFF22_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019649019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX18540.1"
                     /translation="MTVHSQFFNNRSGLRLHVLCWGNPQGTPLILLHGLRAYAQTWES
                     LAQALGDDFCCYALDQRGRGLSDWAESSSYHTQSYVHDLEDLVAHLELQHFALVGHSL
                     GGANALEYARQNPGRLLALVVEDIGPGSSSVGDGAERIRREMRQTPLLFADWEAARAF
                     WVKSRPGLSEQGLRLRLAHSMRETAQGIVWRHDQLGIADARLSIEPVDLWPAVRALDC
                     PTLFVRGGNSDFLPPATLAAMQEQNSNIRTVEIAGASHYVHDDQGEAFNRTVAGYLRD
                     MTKPSE"
     gene            complement(111606..112208)
                     /locus_tag="VFF22_16945"
     CDS             complement(111606..112208)
                     /locus_tag="VFF22_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003172904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HZX18541.1"
                     /translation="MNPSNFANYHIRKWYSFTEETLANESGQLADGEPLFKYVIAAAV
                     HNPYAGRFSQDLSALIDDSPALGEEFGRRIRELAGERKILSYGKACLVGANGEYEHGN
                     AFLTNPAADPIRVALGGGKSWVPSTGKRGAPGCTIDVPLAHKDALYVRAQYDTVSVTF
                     GDGPAPDEVIIIWAFATRGRLHARLGGLRAEDIKGDNGLT"
     gene            complement(112219..112668)
                     /locus_tag="VFF22_16950"
     CDS             complement(112219..112668)
                     /locus_tag="VFF22_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003200799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZX18542.1"
                     /translation="MKVEIVRTHLSLFVTDPNASALWYADVLGMHESARGEAWVMMAF
                     GSKHHDIALIKAAPGAHQGGLGLQHYGLEIAGDMTTLRQLYGMLLSKGVEVVKITDHE
                     IGNGLYFNDPDENRLEFFLETDHDDDRAKARFKAACAPSRNITLDPL"
     gene            complement(112679..113719)
                     /locus_tag="VFF22_16955"
     CDS             complement(112679..113719)
                     /locus_tag="VFF22_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003172902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HZX18543.1"
                     /translation="MSNGTFLVATVGQAIIRSADNGTTWHRQGLGQPLEFDAITRSLS
                     LDPQQPEVIYAGADIGLIVSRDVGSHWELVDAPFNGQTVWKVAVDPQDSQRIFVGTGA
                     PSRAVLWRTLDGGQSWYRVPVEIPEFCEGVSKPRLLAFAYDPTDRNQVWFGLEEGGLF
                     HSRDGGDTFTRVDERLLWDYNSDIHNILVMPNHGKKIIVVVCVNAIYRSEDEGLSWSG
                     QLAKDDFGLYYVRAMNAPLGSEDTVYLSISDGTPGTTSKILVSRDAAETWEVLPLPQQ
                     PNSCVWVIGINPNNPKQIIAGTKYGHLFTSANGGDGWLKQWRDFSEIADVLWTPAVAQ
                     IKPAHTSVASKK"
     gene            complement(113733..114527)
                     /locus_tag="VFF22_16960"
     CDS             complement(113733..114527)
                     /locus_tag="VFF22_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007933800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX18544.1"
                     /translation="MDLQLKDRVAIVTGGGMGIGKEVARFLSQEGCKVVICARRMEYL
                     KQTAEEISAETGNEVLPLFCDTNEMSAVAEMVAETKRHFGRIDILVNGAAAPSGVVRN
                     DIEHAGDDELLSDLNTKVIGYFRCSKAVTPHMKELGFGRIINIGGLTGRGSKVLSGMR
                     NLAIAHMTKTLSDQLGPAGITVNLIHPGVVDTPHIQELYEREGVKQGKTPQQVEQGYI
                     DATPIRRTLQPIEMGWLIGFLASPKAGSITGESIGIDGGLTRGIFI"
     gene            complement(114553..116043)
                     /locus_tag="VFF22_16965"
     CDS             complement(114553..116043)
                     /locus_tag="VFF22_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019362891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZX18545.1"
                     /translation="MSDIELLPAVREFLSTPRRLFIGGSWQDAALGQAFAVENPATEQ
                     VLCEVAEASETDVDAAVAAARAAFEGPWRRETPARRGLLMMRLADLLEAYAEELAQLI
                     TLENGKPIANARGEAASAANIIRYFAGWPTKIEGSTVPVSPASGAPMLNYTLREPVGV
                     CALIVPWNFPLTMCVWKLGPVLATGCTAVLKPAEQTPLVAVRLVQLIEQAGIPAGVVN
                     LLTGLGVRTGAPLAQHADVDKIAFTGSTQVGRLIAQAATSNMKKVSLELGGKSPNIIL
                     PDADIARAAKGAADGIFYNQGQVCTAASRLYVHESVLDQVLAELRRHATAYVLGSGLN
                     PANSMGPLVSGRQLNSVSGYLQRGQAEGAELICGGKRPEHLDKGHFIEPTVFLDRAEQ
                     ACVAREEIFGPVVTVMSWSDTDELVSRANDSPFGLAAGLWTRDLRAAHRLAAELKAGS
                     VWINCWNVVDPASPFGGYKQSGWGREMSKHVIDAYTETKSVFVDLA"
     gene            complement(116048..117592)
                     /locus_tag="VFF22_16970"
     CDS             complement(116048..117592)
                     /locus_tag="VFF22_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003200794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HZX18546.1"
                     /translation="MNGAELLIKAAVASGVEYCFANPGTTEIPLVAAMGTEPALKPVL
                     SMFEGVCTGAADGYGRIAGKPAMTLTHLGPGFANGIANLHNARRANTPIVNVIGDHAS
                     WHGNYDPPLASDIEALAGTVSGWVRTSRHAQRIGEDLQCAVQAAWQPRGQIASLILPM
                     DLQASAVTAEALRFAPMQPPIRRFAGDKVERVAAELKAGKRLVFIVGDNGLTAAGLEA
                     AGRLAQLSGVRLFAETFPRVSHRGGGLPDLDRLPYFPEVAIEILDQFDLVVCAGVPEP
                     ISYFGYEGIASRLAERDRLLSLAEVGDDVVGALRALADALGAPAHVPSVQGIDLPPAD
                     EALTPQSVGRVLAAALPHECIVSIEGGTCGYPFFTASAGAARHRVLTNTGGAIGQGIP
                     AGFGAALAERGNTVFCLQSDGSAQYTIQTLWSIAREQLPVVILIAANHRYAILQNELR
                     RFGVTEFGPQALALTELDRPRVDWKALAKGYGVAASSVRSNAELQQALARAIASAAPC
                     LIEMEL"
     gene            117805..118575
                     /locus_tag="VFF22_16975"
     CDS             117805..118575
                     /locus_tag="VFF22_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018927853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZX18547.1"
                     /translation="MSSLSKTLGVLDLFGPQQLQLDPETIAERMGLSRATVYRYVKDL
                     CEAGLLSRVGAGNYALGPRIIELDWMMRQYDPILIAGREPMQRLSEETGLAVFASVFY
                     DGHIINTYITDPNEQHHFAFGRGHPLPPFRGAQSKVLVAHQKGRRLLKLFEEQISKDP
                     NNPYSTRSWSEFARAAKKIRKDGYCITHDELNLGLTGIAAPIIVMDGKEVVGSVSAVG
                     TTDTFKLLRQERVIDLVIQTAKLIATNMQKPQSTSGPS"
     gene            118763..>119850
                     /locus_tag="VFF22_16980"
     CDS             118763..>119850
                     /locus_tag="VFF22_16980"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZX18548.1"
                     /translation="MKLNHTNLLREQAYIDGQWVDADNQALFSVTNPANGELIAMVAS
                     VGQAETARAIAAAKAALPAWREKTAKERSAILRSWYNLIMENQEDLARLLSWEQGKPL
                     AESRGEIAYGASFIEWFAEEAKRIYGDVIPHDKQGRRLVVIKQAIGVVAAITPWNFPN
                     AMITRKVGPALAAGCTVVVKPASETPLSALALAVLGERAGIPAGVLNIVPGTDSRAIG
                     GELTGNPDVQKLSFTGSTGIGKLLMAQCAATIKKVSLELGGNAPFIVFDDADLDAAVQ
                     GALGSKFRNSGQTCVCTNRFLVQNGVYEEFSQRLVAAVNALKVAPADEDGAQQGPLIN
                     ANAVAKVEEHIADAVSKGAKVLAGGQPHA"
BASE COUNT        24032 a        37969 c        35881 g        21968 t
ORIGIN      
        1 gcgaagcgct cgagcagcgg gaacagcacg cttgcgtcgg cgaggcgagg caacggcaca
       61 gactgccggc cattgcactc aacctggatc acccgctgga tgggtggcca cgcctggcac
      121 agccagtcgg gtagttcgat agccggcctc cgcaggtcag gacaagaaaa caatgagaac
      181 gattagcgtt gatggtatcc ccgcctctct ataaactcaa cgcgtttcac cctgggcaac
      241 ttgaggccac atgcagacat tacgtgattt ctggttgctt gcccgaccct tctggtcttc
      301 taccgaaagg cgtcccgcct tcctgctgct gttcggcact gttctgatga acctgtgtct
      361 ggtgggggta aacatcctct acaacatctg gaacctgcac ttctacaacg ccctacaggc
      421 cctcgactac gacgcattcc tgatcggtgg tgggcatttc atcctgctgc agctgggcct
      481 ggccaccttc accgtggccg cctttcactt ccaacagaag ctcaccgtgc gctggcgccg
      541 ctggtccacc gagaacacct tgcagctatg gctgcagcgc catcgctatc tcaaactgca
      601 gctcacgtcc ccggaaaccg acaaccccga ccagcgcatc gctgacgaca tcaacctgtt
      661 catcagcatg tcgttgaagc tcaccttggg gctgatgact gcgttggtgt cgttattctc
      721 ctttctgcac atcctctggc aggcttccag tctggtcaaa ctacccatcg ctgggcagga
      781 agtgatcatc cctggtctgc tggtgtgggc ggccctgctg tattccatta tcggcacggg
      841 cggcgccttc tggctggggc gtaccctgcc ccggctgaac tttattcagc aacggcgcga
      901 agcggatttc cgcttcgccc tgatgcgcct gcgcgagaac gccgaagccg tagcccaata
      961 ccgcggcgaa aacgaggaac acgcacgctt cagtcgacgt ctgggcgctg cgctggagaa
     1021 cttctgggcg ctggtgaaac aacaaaaagt cgtgcttggt tattccacct tctatatgcg
     1081 cagcgccatg atcattccca tgttcatcat ggcgccgcaa tttttcgcgg ggactttccc
     1141 gctgggccgg ctcacgcagc tcagcgccgc cttcggcgag gtccacgagg ccatggccta
     1201 tctggtgcga gtgtttccgg agctcgccga atggaaatcc gtgattgacc gcctgaccgg
     1261 tttccagaaa cgcctgggca gcgtgacaag cgagccaggc gtgcagctgg aacatcgacc
     1321 tggcagcttc gccgtacagg atctgtcact ctggctacca gagggtaaat ctctgctcgg
     1381 cggcctgaac ctgagcctgc aaccgggtga aagcctgctg attcaggccc cttcaggcta
     1441 cggcaaatcg acgttgatcc gcgcacttac cggcatctgg agccacgctt caggtaaggc
     1501 gctatatgac ttggggagcg ttctgaccct agcgcagaaa ccttacctgc cgctcggcaa
     1561 tctgcgcgat atgctctggt accccgcctc gccccatcca gaggacgacc tagcgctgca
     1621 ccgcgtcatg cagcagtgca accttggcca ccttgtggag cacttggata aagagctcga
     1681 ctggtcacaa acattgagtc tcggagaaca gcaacgctgt gcgtttgtac gagttttatt
     1741 aatacaaccg aatgttctgt ttctggatga aagtagctcg gcgctagatg atgcaacaga
     1801 agctcactgc tatgggttgc tcaaacagga actacctcgc acgatcatta tcagcgtcgg
     1861 gcatcgtgct tctttgcagc gtttccatac ccgaactctg gaccttgagg aatacaaaac
     1921 ccttgagcaa caagaggtgt attcgtgatg ggctagggta atggacgtgc agttttagcc
     1981 aaccacagac caaaccacga agccgccaga tgccgccaac ggattgaggc acctaagcgg
     2041 atcagctacg actagcccga ctagccctgt agcgactcca cacccatctc gtcccacact
     2101 tcctcggcca ggtggaaggt ggcatttgca gccggaatgc cgcagtagat cgcgctttgc
     2161 atcaaaacct ccttgatctc gtcgcgggtc acgccattat tctttgccgc cttgaggtgt
     2221 aggcgcagtt cgccttcacg gttcatgccg atcagcatgg cgatggtaat caggctgcgg
     2281 gtgtgcagcg gcaggccggg acgagtccag atatcacccc aggcatgccg ggtgatcatt
     2341 tcctggaact cttcgttgaa tggcgtcagg tgctgcaggc tgcggtcgac atgcgcgtcg
     2401 ccgagcactg cgcggcgcac ttgcatgcca gcgtcgtaac gttgtttttc gtccattggg
     2461 tgaactccga aggtagcggc gcgcagggcg ccacagaatg ctcaagcagt aaggaaatcc
     2521 agcacgcggc gagtgaaaag ctcactcgct tcaacgttgg acaggtgcgc ggcatgcagc
     2581 tcgaccagtt cggcagcgtg aatgcgttcc tgcataaagc ggccatgctc gggcgtagtc
     2641 accccatcgg cagtgccgca aacgatcagg gtcggcgcaa caataccggc aacctgctcg
     2701 cggaaatcgg catcgcgcac cgccgcgcag ttggcggcgt agccctgcgg tgaggtggcg
     2761 gccagcatgc caaccacggg ctcgacctta gctggctgag cttgagcaaa ttgcgccgtg
     2821 aaccagcgcg caatcgaggc gtcgcgcagc gccaacatgg cggcctggcc gtcgcgcagt
     2881 acggtttcga tacgtggatt ccatacctcg gggctgccga tcttcgctgc ggtgttacac
     2941 agcaccaagc gctcaatacg cgccggggcg ttgatcgcta gccactggcc gatcaacccg
     3001 cccatggaca gtccacagaa ggccgcactc tcgatatcca gcacatccag caaggccagc
     3061 acgtcgcggc cgttctgctc gatgctgtaa aagccttcgg tgaccagcga gcggccatgg
     3121 ccacgggtgt cgtagcgcaa cacgcgaaaa tgttcggtga aggctggcat ctgcgcgtcc
     3181 cacatatgca ggtcggtgcc caacgagttg gacagcacca atactggcgc atcttgcgga
     3241 ccgtcaacgg tgtaataaag ttcgccatca gcgagttgta caacagacat gcaaagcact
     3301 cctaatacga gaaattctga tgttcggcca ctgccctctc gacccagcga cgggcttgcc
     3361 ccaggtacaa ggccggatca agtagacgat ccagctcggc gccagacagc tgcgcgctga
     3421 cttcggcatt agcgccgagc acctggcgca ggtgtacgcc ttcgcgcaca gcctgctggc
     3481 agcactgttc gagcaaatga tgggcagcgt cgcggccgat gcgttgggcc agggcgatgc
     3541 tgactgcttc ggccagcacc aggccctgag tcaggtcgag gttactgcgc atgcgtgcgg
     3601 catccacctc cagccccggc accagcagca gtgcctgctg caacgccccg gcaaccaggc
     3661 agcacagctc cggcagggtt tcccattcgg catgccacag ccccagactg cgttcgtgtt
     3721 cctgcggcat ggcgctaaac atcgtggcca ccaggcctgg cgcacgcgtg gctgcgctga
     3781 tcagcactgc ggcactgacc ggattacgtt tatgcggcat ggttgaagag ccgcctttac
     3841 ccggtgctgc cggctcaaag acttcgccag cttcggtctg catcaacagg ctaaggtcgc
     3901 gaccgaattt acccaggctg ccggcgatca gaccgagcag actggcgaat tcaaccaaac
     3961 gatcacgttg ggtatgccag ggctgctccg gcagttctaa atcgagttcg cgggccaagg
     4021 cttcgctcac cggccaagcc tgctcgccca atgccgccag gctgccggag gcaccaccga
     4081 actgcagcac cagcaggcgc ggtttaatct cggccaggcg ctgacgatgg cgggtaatcg
     4141 cgccgagtac gccggccaac ttcatgccca acgtcaccgg tgtggcgtgt tgcagccagg
     4201 tgcgtccggc cagcggcgta tcggcatagc gctcggcctg cacagccaaa gccgctgcca
     4261 gttgcgccag atcctgctcc agcagcgcga tagctgcccg cagttgcagg atcaaaccgc
     4321 tgtccatggc gtcttggctg gtagcaccga gatgcacgta acgctcggcg tttgtgcttt
     4381 gcgcagcgac ctgcttgccc agtgctttga ccaacggtat ggccgagtta cccgccgtga
     4441 cgatggcctg agccagcgca gcaaagtcat aacgctcggc ttggcaagcc gtggcaatcg
     4501 gccccacaac ctcggcaggg attagcccgc agctcgcttg cgcgcgcgcc aaagcggcct
     4561 cgaagtcgag catgccttgc acccggccgt gatcagaaaa aactgcgcgc atagccgctt
     4621 ggctgaagta ggcgtcgaag agttggttac tcaagagatg tattcccaaa ggcgcttgat
     4681 tacagcctag gttcgcagca ctggcgtgac gaatccagcg cttgtaacgg cggcatgggt
     4741 atcgctgcgc tcaacctacg cggcccgacc catcctacgg aataacatcc cgtaggatgg
     4801 gttagcccca ggcgtaaccc atcgcgatgt cagttaggct caaacgcgtt ctattgccag
     4861 cgccaaaccc tggccaacac cgacgcacat ggtcgccagc cccaggcggc cgccggattt
     4921 ttccaactga tgcaacgcgg tctgcaccag gcgtgcgcca ctggcaccca gcgggtggcc
     4981 gagggcgatg gcaccaccat tcgggttgac cttagtgctg tcgtcagcca atcccagctc
     5041 acgggtcaca gccaagcctt gcgccgcgaa agcttcgtta agctcaataa cgtcaaagtc
     5101 atggatgctc attttcaggc gcccgagcag cttgcgcacc gccggcaccg ggccgatgcc
     5161 catcacccgt ggtgccaccc cggcactggc catacccaac accttggcac gggcagtgag
     5221 gccgtatttc tgcacagctt cgcgcgaggc cagaatcagc gccaccgcac catcattaac
     5281 cccggacgca ttgccggcgg tgactgtttt gccctcgcca tttaccggtt tgagtttggc
     5341 cagcgcttcg gccgtagtgt ccgggcgcag atgctcgtcc gcctcgatca cgctgtcgcc
     5401 cttcttgcct ttgatcacta ccgggacgat ttcttcggcg aagaagcctt ctgcctgcgc
     5461 gcgaccggca cgctgctggc tgcgcaaggc aaaggcatcc tggtcgacgc ggctgacctg
     5521 atactcgtcg gcgacgttgt cggcagtctg cggcatggcg tccacgccgt actgcgcttt
     5581 catcaacggg ttgataaagc gccagccgat ggtggtgtcc tcaattttct ggttgcggct
     5641 ccaggcaccg tcggccttgc ccatcacata aggcgcgcgc gacatcgact cgacgccgcc
     5701 ggccaacgcc agctccatct cgccactggc aatggcgcga aaggcgctgc ccaccgcatc
     5761 catgcccgag gcgcagaggc gattgagggt cacgccggga acgctgtccg gcagaccagc
     5821 cagcagcagg gccatgcgcg cgacgttgcg gttatcttcg ccggcttggt tggcacaacc
     5881 cataaacact tcgtcaatgg ccgccggatc aagctgcggg ttgcgttcga gcaaggcttt
     5941 cagcgggatg gccgccaggt cgtcggcgcg caccgctgcc agaccgccgc cgaaacggcc
     6001 gatgggcgtg cgaatggcgt cgcagataaa tacctcgcgg ctcattcctc acctccggct
     6061 tggccgtggg cagcagcggt acgcgcttcc agggcacgca acgcttccag ctcaaacgcc
     6121 tgaggcgctt cagtctcagt tacctgctcg gcaaaacgaa tctgccagcc agtcgcggca
     6181 acgatctgct cgcggcttac gcccggatgg atcgaggtga ccacgaactc cttgctgccg
     6241 gcttccggct ccatgatgca caggtcggta atgatcgcca ccggcccagc gccgggtagg
     6301 cccagctgct ggcgatgctc gccgccttcg ccatggccga ccgaggtgat aaaggccagc
     6361 ttgtcgacaa aggtgcggtg cccctgtttg aggatgataa acaccttctt cgctgagcct
     6421 gcgatctccg gcgcgccgcc ggcacccggc agacgcactt tcgggttgtg gtaatcgcca
     6481 atcacggtgg tgttgatatt accgaacttg tcgacctgag ccgcgccgag aaagcccacg
     6541 tcgatgcgcc cgccctgcag ccagtagcgg aagatctcgc cggtcggcac cacggtgtcg
     6601 gcggtctcgg ccagctcgcc gtcaccgatg gacagcggca gcacggtcgg cttggcgccg
     6661 atggggccgg attcgtagat cagcgtcaca tccggggcat gggtcaggcg cgccaggttg
     6721 gcggccttgg acggcagacc gatgccgacg aaggtcacat ccgtattact gaggcgacgc
     6781 gaggcggcca cggtcatcat ttcattggtg ctgtaggcgc tcattacttg gcctcctgaa
     6841 gcttggcctg ataggcggcg aaatcggcag tgccacggat ataggtgtcg atccaggcag
     6901 tgaaggtttc gcggtcgcgg gcgatcgggt cccaggcctg gtagaagcgg ttgtcgcgct
     6961 cggagtaacc gtgggcgtag gacggatgcg caccgcccgg caccaggcac acggcagtca
     7021 aggcccagcc cggcagcacg caggcgttca ttggcgcgtt cagctcgtca acgatttctt
     7081 ccacggtgac gatgcaacgc ttggcggcca atgccgcttc cttctgcacg ccgaggatgc
     7141 cctgaatcag cacgttgccc ttgcggtcag ccttctgcgc gtggatcacg gtgacgtccg
     7201 ggcgcacgct gggcacggcg gccagttgct caccggtgaa ggggcactgg atgaatttga
     7261 tgtccgggtt gaccttgagc agatccgagc cctgatagcc gcgcagcacc gcgaacggca
     7321 gacccgaggc accggccaca taggcgttgg ccatggcggc gtggctgtgc tcgctgatct
     7381 gcagcggctg cggccagccc ttctccaccg cgtcacgcag gcgatgcagc gaacccacac
     7441 cggggttgcc gccccatgaa aagatcagct tcttggcgca gccggcaccg atcaacaggt
     7501 cgtaaaccag atccggggtc atgcggatca gggtcaggtc cttacgcccc tgacggataa
     7561 tctcgtggga cgccgcagtg gggatcaagt gggtgaagcc ttcgagggcg atgctgtcgc
     7621 cgtcgtgaac gaagcgctgg atggcttcgc gcagagtgag gatttcagcc atggggattc
     7681 tcgtatggtg tcgtggcgtt gctacgccgg tgaacagacg ttaacgccgg gtctgcacgc
     7741 aaacaatccg ataatcgact attggttcgt ttatcgaaca catctgtcac gttcagctaa
     7801 ggccgggcgc agaggcccgg cccgtcgggt ttaaagcagg ttccaggtgt agttgaagat
     7861 cagccggttc tcgtcgatat cgctgcggta gttggagcgc gcggtaacgt tgcgcacgcg
     7921 cacgccgagg cccttgagcg ttccgctctg caccacatag ccaatatcca ggtcgcgctc
     7981 aaagtccttg ccctcatagc gtgggccgcc acggttggtg gcctgggcgt cgacgttatc
     8041 accacggata tagcgcaccc cggcaaccag gccgggcacg ccgatggcgc tgaagtcgta
     8101 gtcataacgc acctgccagg agcgctcgtc ggccgaggcg aactcgtagg tcggcacctc
     8161 gttgcccagc ggcgtgatgt tggcaaacac ccgtgggaag gcgctgtcgc cgtacatggc
     8221 ctggtagccc aggtaaaagg tgtgaccgcc atgcttggcc gataacaggg caaacagcgc
     8281 ctggttgtcg atgtcgccga gcaggcggct gccatcttcc tgggcatcgt agtagccaag
     8341 gttggcaccc agggtccagg tacccagcgg ctggctgtgc ttgagaccaa ggaagcgctg
     8401 gttatagata tcttccagct ggccgtacca ggcactggcg gtgctgcgcg tggcattgaa
     8461 ggtgtagtcg ccaccggcat agttaaaggc gtcgctgacc gcaccacgct gcggtaggtg
     8521 gccaagcatg gccagcattt tgtcgtcgcc ggcctcgttg cgcaggctgg tggagttgag
     8581 gtggccggcc tgcagggtta gcccggcaat ctcgctggag gtaacgttga gcccctgata
     8641 gctcggcggt agcaggcgga tatcggaaaa cgccaacacc ggcagattcg gctgtagctc
     8701 acccaccttc agctcggtcc tggagatacg catcttgccg gtcaggccca ggcggctgta
     8761 gtcatcggcc gcttcgccat cttcctgtag cggcagcaag cctgtgttga ctcggccgcg
     8821 gccgctgtcg agcttgatgc cgagcaggcc gatggcgtcc acgccaaagc ccaccgggcc
     8881 gtcggtgtag ccagatttga tgttgaggat aaagccctgg gcccactcct ccgccctgga
     8941 ctgctgactc ggcccgacga tgtcggaaaa atcacggctg aagtagtaat tgcgcgcttg
     9001 caaagtcgcg ctggtgtctt cgataaaacc accttcgcca gcctgactca ggcttggcag
     9061 tgcggccgca atggccaggg gcagcagacg aagggaggta atggagttgt cgttcatggt
     9121 tggctcttgt tgttatttca ggttgcagaa acccgccccg gcgcgctgga cgagccggtg
     9181 atgcaagaca tggcgggagt tatatgagcg gccgccaggg ccactcacaa aggggaatcg
     9241 gattgatcag ggtgcagggt cacccacaga gcccgcagtc ggcacagcgg cgctcgccgt
     9301 agcgcgtcga gtcagcagca ggtgtgcggc gatgccgaac atcaatcccc agaaggccgc
     9361 cgacaggccg agaaaggaca tgcccgaagc cgtcactaga aaggtgatca gcgccgcttc
     9421 gcggtcatcc ggctgggcca tggcactggc cagcgaaccg gcaatgacgc cgaacagcgc
     9481 caacccggcc agggcggcga ccaactcttt ggggaaggca ctgaacagcg acaccagggt
     9541 tgcgccgaac aggcccagta gcagatagac caggccgcca accaccccgg cgacatagcg
     9601 ccgggccggg tcttcgtggg cttcgcggcc ggtgcaaatg gccgcggtaa tagcagccag
     9661 gttgaggcca tggcaggcga agggggccag caacagcgaa cccagggcgc tgctggcgat
     9721 gatcgggctg gccggggtcg ggtagccggc attgcgcagc accgccatgc ccggaacgaa
     9781 ctgcccggtc agggccacca tcaccagcgg caaggcgata ttcagggtcg ccgacaggct
     9841 gaactcgggg ctgatccata ccggtgtggc cagaccgatc accaatgcct cctggcgcag
     9901 gtcgccggtg acaaaggcga tgctgcaacc gaccagcagc accgccagta ccgcatagcg
     9961 cggcagcaca cgcttgacca gcaggtagct ggcgaacatc gccagcacca gccacggctt
    10021 ggtctgcagc gaaacgaata ggccggtgcc gaaactgaac aggatgccgg ccagcatgcc
    10081 ggcggcaatc gctgcgggca gcctgttgat gatgcggtca aaggcaccgc tgatgccgac
    10141 cagaaagatg atcaggctgg cgaccatata ggcaccgacc gcctcattca ggctgatctg
    10201 cggcagtagc gacaccagca acgccgagcc gggtgccgac caggcgatga tgattggcac
    10261 ccggtagcgc aggctgagga cgatacccag caccgcgcta ccgatggaaa tcgcccagac
    10321 ccaggacgac agcacggcat gcgacatgcc cgcgctctcg gccgcctgga agatgatcac
    10381 caggggcccg gcataggaaa tcaccgtggc gataaaaccg gccaccacgg cggacaggga
    10441 gaaatcgttg atcaaggctt tcatatggcc tcgcaaggta tcaatggcgc gcctcgggga
    10501 gcgctctcgt tgtatgtggg ccccgtgacc gcggcttgaa ccggctcaca cgaggccaac
    10561 ggcgcgcggc atcaatggtg cgtgcggcgc accctacgcg ccctgcaacg cacgcggacg
    10621 cttgctgcgc tgttcggcct gggcggccgg ggctttctgc agctggaagt cgaactgcag
    10681 ttcggcgaag cggctgcctt gcacgccaca gtcacgggcg gctgcggcgt cctcgacaaa
    10741 tttgatatcg ccaaccaggc cctcacgggt ggcgtaggca aagtcgtccc acaggtactg
    10801 ttcaccggcc aggttgatct gggtggtcag gtggcgatgg cccggcgcgg agataaagaa
    10861 gtggatatgc gccggacgct gaccgtggcg gccgagcagg tccaggcact cctgggtcgg
    10921 gccatctggc gagcagccat aaccactcgg cacaatgctg cgtgcgcggt agcgaccctc
    10981 ggcatcggtg acgatacggc ggcgcaggtt gtactcgctc tggctcttgt cgaagaagga
    11041 gtagttgccc tgggtattgg catgccacag gtctaccacc gcaccggcca caggcgtgcc
    11101 ctgggtgtca cgtaccacgc cttcgaggaa catcaccgtg gcggagtcca gctcgctgcc
    11161 gtcgtccatg cgtacttcac cttcggccag cggcgcaccg gcgacataca gcgggccttc
    11221 gatggtacgc ggggttccgc cggtgaggcc ggcctgggca tccttggcat cctgcagcag
    11281 gtcgaggaag tgctccaggc ccaggcccgg caccagcagg ccggcctcat tgcgcccgcc
    11341 caggcggttg aggtagtcca ccgcgtgcca gaactcgtcg tcactgattt ccaggtcttc
    11401 gatgagcttg gccgcgtcat tgatcagacg cagcatgatc tgcttgaccc gcgggctgcc
    11461 ctggtcgttg ttaaggccgg cggcctcgtt gaacagcttc tggacttcgg cagtgtggga
    11521 gacgttcacg gtcatgatga tttcctcgga atattgttat cgggcttagc ggtcgtcgct
    11581 atgaatcgat gagggatggc ggcagacgcc attgatttcg atatccatat agggaaacag
    11641 cggcagctgc agcagggtgt cgtgcagggc ctcgacgctg gccacatcga atacgctgta
    11701 gttggcgtag tgcccggcga tgcgccacag gtggcgccac ttgccttcgc gcatcaggcc
    11761 ctgggccagt tccttctcgt cagccttgag cttggcggct tgcgctggct gcatgtcggc
    11821 cggcagtttt acggtcattt ttacgtggaa cagcatgggg atttcctccg gttcaggggc
    11881 ggcggaagaa cgccaggcgc tcttcatcca gggtcaggcc gaggccgggg gtgcgtggca
    11941 cctgcagggc aaagtcgcgg tacaacggcg gttcgctgac gatgtcctcg gtcaacagca
    12001 gcggaccgaa cagctcggtg ccccaagtca gctggcgcag ggtgacgaag gcatgggccg
    12061 aagccagggt gcctatcgcg ccttcgagca tggtgccgcc gtacagagcg atacccgccg
    12121 cttcggcaat tgccgcagta cgcagcacgg ctcgcgggcc accgttcttg gcaattttca
    12181 gggcgaagat gctcgcggca ccgtcggcgg ccaggctgaa agcgtcttcc acgctttcaa
    12241 tcgattcgtc ggccatgatc ggtgccgggc tgcgctgatt gaggcgcatc tgccctgccc
    12301 ggttgatccg cgcaatcggc tgctcaatca ggtcgatacc gttgtcgccc agcacctggc
    12361 aagcgcggat ggcctgggac tcatcccagc cctggttgac gtcgacgcgc acgctggcgc
    12421 tatcacccaa tgcccgcttg atcgcaatta catgcttgag atcctgctcg accgggttgg
    12481 caccgatctt cagcttgaag atgcgatggc ggcgcaggcc gagcatctgt tcggcctcgg
    12541 cgatgtcgcg ggcggtatcg ccgctggcca gggtccaggc cacttccagg ctgtcgcgca
    12601 cgcgaccgcc aagcagctcg ctgactggta ggccgaggcg tttgccctgg gcgtcgagca
    12661 gcgcgctttc caggccggac ttggcgaagg tgttgccctt ggcggccttg tccaggcgca
    12721 acatgcaggc gttgatattg gccgcgtcct gaccgatcag cagcggcgcg agatggctgt
    12781 cgatgttctg tttgatgctc tccgggctct cgttgccata ggccaggcca ccgatggtgg
    12841 tcgactcgcc gatgccttcg atagcgtcgc tgcaacgcac gcgaatcagg accagggtct
    12901 ggttctgcat ggtgtgcatc gccagcttgt gcggacggat ggtcggcagg tcgacgatgg
    12961 tcgccgccac actttcaatc aggacttggc tcattgcagg ggttccctgt tgcgataaaa
    13021 gacgtcaggc cttggccgcg ttcagcgcca tagatgcgct ctgccggcgc ttggcgctga
    13081 tggcgaacac ggtcatggcg atggcagcga tggcgccggg tacggcgaag gcgagaaaat
    13141 tcagttgcag cggcaggttg atgcccatca gtgcgccgcc cagcagcggg ccaacgattg
    13201 caccgttgcg gccaatgcca gaggcccagc caaggccagt ggagcggatc gacaggccgt
    13261 agaactgcgc ggcaccggcg tacagcagaa tctgcgtacc gatggtggtg gcaccggcga
    13321 taaagatcag gccatacagc agcggcgtcg ggctcttgaa accgagcaag ctgatcgaca
    13381 cggcagcggc aacgaagaag gccaccatca ccttgctcag gttgaaacgg tcacccagcc
    13441 agccgccgag aatggcaccg gccatgccgc cgaagttcag cgccaggagg aacgacaggc
    13501 tcgaccccag gctgtagccg gcgttggcca tcagcttggg cagccaggaa ctcagtgcgt
    13561 agaccatcaa caggcagcag aagaacgcca gccacaggca cagggtgcgc agtgcgcggc
    13621 cgtcacggaa caactcgaat accgcaacgc cctggccctt ggtgtcactc atctgcagtt
    13681 ggtcggccgc gtcaatctgc aggccaggct ggatcttctg caacaccgca cgagcctgtt
    13741 catgacgccc ctggcgcacc agaaagccca ccgactctgg caatttccac agaatcagcg
    13801 gcaacagcag cagcggaatg gcggcggcga agaacatcga ctcccagcca aagcgcggca
    13861 acatatagat gccgacaccg gcagcgagca tgccgcccac cgagtagccg ctgaacatga
    13921 cggccaccag ggtgctgcgc aggcgcttgg gcgcgtattc attcatcagg gctacggcat
    13981 tgggcatcag accgccacaa ccaagccctg cgagaaaacg gcagatgcca aactcggtcg
    14041 gattgctggc gaaaccgttg atgatggtgg cgctggaaaa cagcgcaaag cagatggcga
    14101 tgcccttctt acggccgatc ttgtcggcca gggtgccgaa agccagggca ccgaacatca
    14161 tgccgaacag cgcgtagctg cccagcgcgc cggcctgcag cggggtaagg ccccactcct
    14221 gcatgatcga cggcagtacc acgccgtaga taaacaggtc gtagccgtca aagatcaaca
    14281 gcagcgcgca gagcgccata accatccagt ggaagctgct aaagcgcgcg ttgtcgatga
    14341 cttggtgaac gtctatctgt cgcatggcat gggttctctc tgttgttttt gttgtgaaaa
    14401 gctcgttgaa gcaaatagcg gtatttctgt aggagcgagc ttgctggcga tgctcttaat
    14461 ggatcgcgag caaggactag gcgtccccct ggctcctaca caatcaggta ctaacccaga
    14521 tcccctccac ccaccggcag agtcacgccg gtgatgtagg aggcctcgtc ggaggcaaga
    14581 aaaaggatgg cgccgacctg ttcgtcgatg gtgccgtagc gcttcatcag gctgctggcc
    14641 agggtctgat caacgatctg ttgataccaa cgctgctcgt ccgcgctcgg ttcagcgctg
    14701 ttgcgcggga tgcgccgtgg tggtgcctca gttccgcctg gggcagtggc attgacccgg
    14761 ataccgcgct cggcagtttc aaaggccagg caggcggtca gtgcattgac cccgcccttg
    14821 gccgcgccat agggcacacg gttgaccccg cgggtggcga tggacgaaac gttgacgatg
    14881 gcaccactgc cctgcgccag catctgcggc agcgcggcgt ggcagcacca cagggtgggg
    14941 aacagcgagc gacggacttc cgcctcaatt tgctcggtga cgtagtgctc aaacggcttg
    15001 gcccagatgg tgccgccgac gttgttaatc aggatgtcga ggcgaccgaa acgctcgacc
    15061 gctgcctgca tcacccgctg gcagtcatcg gcccgctcca ggtcagcggt caggcacagc
    15121 acagctttat gttcggccag ttcgaacacc agctcggagc ggtccaccgc cagcaccagt
    15181 gcgccctctt ccagcagccg ctcggcgacg cggcggccaa tgccttgagc agcaccggta
    15241 accacggcaa ctttctcttg aaaacgcatc ttcatagttc cgacctcaat caatacttgc
    15301 ggcgcgcctg caacctgtag gagcgggcta gcccgcgatg cttccaacgc cttcgcggac
    15361 aagtccgcgc ctacaagagc gaggcgtcat gcggacgcgg caaacttctc gtagtagaaa
    15421 ttggccgggc tgatgccctg ctcgcggata tagccgctga ccgcttcgac catcggcggc
    15481 gggccgcaca ggtaaatgtc tacctcgccg tcattcaggt gccggggttc gatgtgctgg
    15541 gtgacgtagc ccttgtgcgg atagctgcta tccggattgg ccacacaggc gctgtaggta
    15601 aagttgggga tgctggcggc cagtgcttcg agccggtcca tctcgaccag gtcgaagtcg
    15661 ttggtcaccc cgtagatcag gtgcagcgga tgctggctgc cctcggcggc aatcttctcc
    15721 agcatcgcgg taaagggcgc gaggcccgtg ccgccggcca gcagcagcaa tggccgcttg
    15781 atctcacgca ggtaaaagct acccaagggg ccggccaggg tcatgctgtc gccagccttg
    15841 gccaaaccgg tgaggaaact gctcatcaac ccgcccggca cgttgcgaat caggaagctg
    15901 acttcgccat ccttgggtaa tgagctgaag gagtaggcgc gggtttgctc gctgcccggc
    15961 accttgaggt tgacgtactg acccggcaag aacgccagct ggttgagtgc ctcgcccttg
    16021 atcgacaggg cgatggtgct atcggatagc tggcgcaccg cgctgatcgc cgcctcgaag
    16081 ctggcctgct gggtcttgca cacctcgctg gacgccggca cgcggaccac gcaatcgctc
    16141 tgtgcacgca tctggcaggt caaaacgtag ccctgctcgg cttcggcctc gctcagggcg
    16201 tcttcaatgt attcctcacc gaggtcgtac tggccggctt cggcgaaaca tttgcaggcc
    16261 ccgcaggcgc cgtcgcgaca atcaagcgga atgttgatgc cctggcgata ggccgcgtcg
    16321 gccacagttt cgccgagctt ggcgtcgata aaacgggtaa ccccgtcttc gaaattaagg
    16381 gcgatcttgt ggctcatggc acacctcgcg aaagtagttc tggccgatag cggcgtgcag
    16441 aggccctagg cgggaagcca tgcctcccgc cggctgctct gcaacggatt cagatgtggt
    16501 aaacgtcgat gatctgccgt acgtagtcgt tcttcagcac caccttcttg gccttgatca
    16561 acggattggg cccgttgcag tcgagggtgt agaagctggt gccgtagaag tgatcgacgg
    16621 tcttgtagcg aaagctcagg gtgtgccagt tgaagcgcac ctggcacaga ccatcgacct
    16681 gctcgacgag ctcgatgttg ttgatgttgt gcgaggtgcg ggtgtccgga atggtcgcac
    16741 tggagcgctc ggtgcggatg cggaacaccc ggtcttccag gccactgcgg ttgccgtacc
    16801 agatcagcga gatttccgtc tgtgggttct ccaccagctc gtcgcggtcg tcccaggccg
    16861 gcatccagaa ggtcgcgtcg cgggcataca gttccagcca ctcgtcccac tgtttgtcgt
    16921 cgaggtagcg cgcctcgcgg tagagaaaat cgcgcacagc atcataggaa aggctcattt
    16981 acgcgccctc cacgtggatc agctggtcct gctgcttgtg cagcgccttg atcatctgct
    17041 gctgccagta gtggtgctgc aatacgaaca gaccttcgtc ctcggtgcgc acaccgctca
    17101 tcagcggatg cagatcgatt tcctgggctg actcgtcggc accgtcgacc cagtgcttgg
    17161 cccctcgcga catatcgttc cactccatgg cgcggccggc aaagccctgc tggcaggcgc
    17221 ggaactcttc caggtcatcg ggagtggcca tgccggaaac attgaagaag tcttcgtact
    17281 ggcggatacg ccgggcacgc gcctcggcgc actcgccttt cggcgcgatg cagtagatgg
    17341 tcacctcggt cttgtccacc gacagcggcc gggcgatgcg cagctgcgag ccgaactggt
    17401 ccatcaggta caggttgggg tacaggcaca ggttgcgtga attgccaatc atccagtcgg
    17461 cgcgtgcttc accgaactcg ccagccaagc gctcgcgctc ggcgaacagc gggcggtcct
    17521 ctgggttatc ccagcgggtc cagagcagca ggtggccgtt ctcgaaggag taaaaaccac
    17581 cgcccttctt gccccagccg cctgcgctca tggcgcgaat gtcgtcgccg gcttctttga
    17641 gtttgcgctg ctgctgggtg gcggcgtagt tccagtgcac cgcggtgacg tgatagccgt
    17701 cggcaccgtt ctcggcctgc agcttccagt tgccttcgta gacgtaggta ctggaaccgc
    17761 gcagcacttc cagaccctcg gcggactgat cacagatcat gtcgatgatc ttgcgcgact
    17821 cgccgagaaa ctcttccagt ggcgcaacat cgtcgctcag gctgccgaac aggaagccac
    17881 gatacgactc gaaacgcgcg atcttcttca gatcatggga gccgtcgcag tcgaagctgt
    17941 ccgggtagcc agcttccttg ggatctttga ccttgagcag cttgccgctg ttgttgaagg
    18001 tccaaccatg gaacgggcac gtgtaggtgg ccttgttgcc actcttgaag cggcagagca
    18061 tggcgccgcg gtgactgcag gcgttgataa aggcattcaa ctcaccgtcc ttgttgcgcg
    18121 cgataaagac cggctgacgc cccatgtggg tggtgtagta gtcgtttttc tcgggaatct
    18181 ggctgtcgtg agccaggtac agccagttgc cttcgaagat gtgctgcatc tccaggtcga
    18241 acaggcgcgg gtcggtgaac atctcccgct tgcagcggaa aatgccctga tctttgtctt
    18301 cttgcagcag gccgttcagg tagtcgagtc ccaggttcat cgccatggct ccgttgttat
    18361 tgtttcaacg gtatgaaggc tatgccgtag gactgtccga caatatccag ttagtgcagg
    18421 acctttatcc gttttctgca aaagccggaa tgccgtagaa gctagtaaat acgcggtcgc
    18481 agacgttcaa gctgagtaag cccgacccaa gatgggcgga ctagagaggg gaatgcagga
    18541 ttggatggtg tgcccgagcg tcgcgttcga ttgcggtgga caagcttcgc gttgtccacc
    18601 ctacctcggc agagggtgcc taatcaggtc tcgcggcgac gcaaggtttc ggagggtaat
    18661 tcgccaaagg cgcttttgta actttcggag aagcgtccga ggtgaagaaa gccgtaatcc
    18721 agcgccacct cggtgatatt gcgtatccgc gaggccgggt cactcaagct gcaatgaatt
    18781 tgctccagct tcttctggcg gataaagctc tttggcgtgg tgccggtatg ccgctcgaac
    18841 agcgcataga gtgagcgcgg gctgctgtgg gccagctggg ccagttgttc gacgctgatg
    18901 tcttgtttga ggttgtcctc gatgaactgc aagatccgct cgaaactggc agcgccctgc
    18961 tccggcacca tccgactgac attgttatcc agcagtgtca gcaacttggt ggcgatgatc
    19021 cgggtgtact gctcctgcac ctgttgcacc gtctgcgaac tctcggcctc gctgcacacc
    19081 agctgcagca agccggtaaa cccctccagc tgctgcaggc tatgccgcga attgaaacgg
    19141 ataccttcgg ccggacgctg ccactggctg tcgcggcagg ttttgtcgag tagggtcgac
    19201 ggcagcttaa cgataaattt ctcgcagtcg tcggaatagg tcaggtcgac cgggtcatcc
    19261 gggttgatca acagcaattc acccggactg aaatagtgtt cctcgccatg cccgcgccac
    19321 agacaatgac cgcgcagcag cacctgcaag tggtagatgg tttccagcgc tggcgaggta
    19381 accctcacgc taccgccata gctgatacgg cacaggtcca gactggcgaa cttgcggtga
    19441 ttgagcgagg cctcaggatg gccgatggtc ggcagacgga tatcgtggca gccgacgtgt
    19501 tcattgacat agctggatac cgcgtagggg tcggcacggt caaacacgca actcgcagcg
    19561 ctgagcaggc aactgtccat caactaggca ctcaatttgt tgttatgtca ctgcgcacgg
    19621 tttcggcgca gttctttatc aggctatggc tgattctatg cgagccagag cggtcgcccg
    19681 cagcggctgg ataaacggtc gcggcgaggc gtcaggcaac acctgcggca aacggcctga
    19741 cagtacgcgg accaggacat gcgactcaat tcaaccttca gccaagtgtg cgattaacgc
    19801 acactgcttg cggctaaacc tctcagtgaa acagtcgcgt gctcagctcg tggctagcct
    19861 ccagcagcac cggcaggtag cgcgactcca gttcctgacg cgatacgcgg ctgacatggg
    19921 tgccgacatt cagcgccgcc agcacctggc cggcggagtc cttcagcggc acggccaccg
    19981 agcgcaggcc gatctccagt tcctggtcga tgatcaccca gccctgctga cggatctcgg
    20041 cgatattcgc gcgcaggcct tcggccgtat ggatagtgcg gctggttttt acctgcaggt
    20101 cagcatgttc caggtaatcg tccagagcca ggtcatctag accggccagc agaatgcggc
    20161 ccatggaggt gcaataggcc ggcagccgac tgccgacgct gagatcgacc gagatcagcc
    20221 gctgcggagt ggcggaacgg gcgatataga gaatctcgtc accctccagg gtggccatgg
    20281 agcaggcctc gtgcagctgc tcgctgatgc ggtcaagaat tggctgagcg gtaactgcca
    20341 gcggcgtcga cgacaggtag gcgtggccga gggtcagcac cttgggcaac aacgaataag
    20401 tgcgcccgtc ggtggtggcg taacccagct tgatcagcgt gtgcagacaa cgtcgcacgg
    20461 cggcccgagg tatctcggtg cggtggctga tctgggcgat ggtcaggtgg cgcttgcgct
    20521 cctgaaaggc atggatcacc gccaagccac gggccaggga agtcataaag ttcgggtcgc
    20581 cggtcagcgc ctcgatacgc ttggccggcg aagcgatgat aggcggtgcc agggtagaga
    20641 gtggcaggcg ggattcttcg ctcattgtta ttgtcctgtg cgtagctgcg agagggctgg
    20701 aagtgcagag ccttgcgcga ttatcgaacc atagtccgat aatcgcaatt tctctctata
    20761 ggcctcagac aaagatagcg ccgcacgcca tcagcccaat ggaccaccca gcacaccgat
    20821 aaaagcgctg acggtcaaaa atgacagcac cgtcgcgccg agcaaggccg ccgcacagcg
    20881 ccccgccagg ccaaaacgct gaccgatgat ggggtagatg ctcatcatcg gcgaacaggc
    20941 gaacagcacc ccggccatgc gcaataccgg gtcctgcaca gggaaccagg tgaacatcag
    21001 cagcaccgcc agcggatgca ggatcagctt gccgacactc acctgcagca catcgccgag
    21061 cagcccgccg gccttgagtc ccaccaggct ggccccgatc acaaacagcg cgaccggagc
    21121 agacgcctgc gccagcatct ccaccacgcg caagggaacc gccggcaggc gcacatccaa
    21181 caacgccagc agcaggccga agctgatggc gataatgatc gggttgcttg ccaggcgctt
    21241 gaaggtgccg agcgctaacg ccttcagccc taccccacgt tgcgcaccgg cctcggccag
    21301 catcagcccc agaggaagca tcagcaggtt ttccaccagc atgcccagcg ccaggcccac
    21361 ggcagctttc tcaccgacga ctaggacgac gatcgggtag ccaataaagc cactgttaga
    21421 catcgacatg cccagggcgg taatggcgct gccactggtg ctgtcgcggc gcaacaagcg
    21481 cgcaaagaga aagccgagca caaacaccgc cagcgagccc aggccatagg ccagcaggta
    21541 gttacggtca aacacctcgt gcagcgggcg ctcaagcagc gccttgatca ccagggccgg
    21601 caaggccagg gtgacgacaa agcggcccat gccggtaacc tgctcagggc tgaccaggcc
    21661 attgcgcgcg gccaggtaac cgatgccgat gaggataaag atcggtgcgg tgataccgag
    21721 aatctgcaac atctaacgcc cctcaattca tgctgacggc gctcagcggc agtgcctgca
    21781 actgctgcag gcggctatgg gtgcgggaca aatcactctg tatgccgcgt gggcacagct
    21841 cgtcaaggct tgcggcgcaa ccgagtaaca gttcgatacc gcggtcgtag gcgatgtcga
    21901 tgaagttcaa aaagcgctgc tgcacatcga cgccctcacc cgccaggcgc ggtacgccgc
    21961 cgatggccag ttcgcgaaac tccgcgcaca accacaggta atcagcactg gagcgcgggc
    22021 gacgaaacag ctcggtaaaa tccaaccagg cccgcgcacc ctcatgacca cgcaggcgca
    22081 acggatgctg gttgaccaac aggttggcgt cacgcgtggc cgcgatgctc aagcccaact
    22141 gagcgcccag ccaatcgtcc tgctcctgca gcggccagca caggcggccc cactgccgag
    22201 ttgagccttg ccggtagtcg tagccgccgt cgagctgcag cacatcaaag cgctgctcca
    22261 acaaggcaat ggtcggctga aaacgcgcgc ggtacagcgg gttggggcac aggcctcccg
    22321 gcgggtaatt ggaggtacaa atcaggccca caccctgccc caccaatagc gtgagcaggc
    22381 gaccgaggag cacggcatcg ccgatatcat gcacatgaaa ctcatcgaaa cacagcaggc
    22441 gcgtctgtcc ggctatctcg gcggcaatct tagccagcgg atcagcctgg ccggcgtaac
    22501 gacccaaacg ctgctgcaac tcctgcagga agacatggaa atgcacccga cgcttttgcg
    22561 ccaatggcgc ggccgcgaag caggcatcca gcacaaagct tttaccgcgc cccacgccac
    22621 catgcaggta gaccccggca gcacggcggc gcaaccagct cgggcgggcc tgcaaaaatg
    22681 cagaaagcca ctggccgaga tggtcaatgg ctgcctgttg cgcaacatcg gcccgatagc
    22741 catgggcctg cagcactgcc tcgaaatgct ggcggacgcg aacgtccgcc cgggtcaagc
    22801 gagaagacac gctagaaatc aaagaacacc gtctcgccat caccctgaat gcgtatgtcg
    22861 aagcgatacg ccggcttgcc atccacttca cagggatagg ccagcaacgt ttcgcggcgt
    22921 tgcggctgct caatcaggtt aagcaccggg tccttcgcat tggcctcggc ctcgtcggcg
    22981 aagtacaggc gggtctgcag gtgaatattg atgccacgag caaacagcga gaggttgata
    23041 tgcggcgcca tcggcacacc ggcggcgttg ttgaccacgc ccggtttgat tgtctgcacg
    23101 ctccactcgc tacccgcatc gaaggtggtg gcggtacggc cgaacccgtt gaacggtttt
    23161 tccaggtcga aggcgctgtc gtaacggcct tcatggtcgg cctgccagaa ctccagaaag
    23221 gagtcgcgca ccaggtggcc attaccgtcg tagacattgc cgatcagggt gatgtgctcg
    23281 ccgggcgcat ccggcttggc catctggctc cagatctcct gagggcggct cgggttaccg
    23341 gcggcctcca gggccaggcc gatatgcaca taggggccgg cggtttgcga aggggtttcc
    23401 ggcagcaatt gaataggcat attcggtgcc tccttagcga ttttcgaaat gagtctgacg
    23461 ctgcccgcgc agcacgatgt cgaagcgata ggccaggcaa tccattggat tggcattggc
    23521 catgtcgagc cgggcgatca gcgtctgcac cgcatccggg ttggcaatcg acttgacgat
    23581 cgggcacatg ggaatcagcg gatccccctc gaaatacaac tgggtgatca gccgggtaga
    23641 aatcgacggg ccactgatgg acacgtggat atgcgccgga cgccagtcgt tcgggccgtt
    23701 gcgccagggg tacgggcccg gcttgacggt gcggaagctg tagttgccct ggctgtcggt
    23761 caacgcgcgg ccgacgccac cgaaattggg atcgagcggg gccaggtagc ggtcattctt
    23821 gtgccggtag cggccgccag cgttggcctg ccacatctcc accaaggtat gcgggatcgg
    23881 cttgccgtac tggtccataa cccgtccggc aacgataatg cgctcgccca ccggcaggcc
    23941 gccgttattg aagttgagca gcaagtcgtt gtcgtgctgg cccagtttca ggtgggagaa
    24001 atccgggccg ctggtttccg agatcgactg cgggatgctc accagcgcct ggcgcggcga
    24061 acgggcaatg gaggtcttgt aatcgggggt gaaggccttg ggatgccagt tgcggtcgcg
    24121 aatgacgaag cggctgttat gcgcatcgga catgtcgatc tcctcttatt ggaatgggta
    24181 caggcttggt acccagtctc aagcagagtc gcacggctga aaattgaaaa gatcgccctc
    24241 atccataacc aaatggttat gaattaaaac tctcgcggta gtccgccgcc gcctcacgca
    24301 aagcctcgca gcaccactgc gcggccagcg acagtgctgc cgtggggttg ctgcagatgc
    24361 ccaccgagcc accgggttcg cgcaggccga tatccagttc cagcagttca ccgcgggcta
    24421 ggtcctggcg cacggcatcc tgcggagcga tccacaccgc atcgctgctc tgcacgtaac
    24481 gccggctaag cgccaccgag agggtttcca ggcgctgctg cggcatcacg atgccgcact
    24541 gcacatagag gctgtcggca tagcggcgaa tcgtggtgcc ggccagcggc aacaccaccg
    24601 ggtagtagtc cagctggcta cggtcacgca gatcggccgc aagcaagggg tgagccggac
    24661 gcacaaccag aatcatcgat tcgctgtaca ggtgctcgaa ggtcaatccc tcgatctgcg
    24721 ggctgtcagt catgcggccg accaccagat ccagcgcgcc gacccgcagc tgatcaagca
    24781 ggtgggcgct gggcccagtg atcacactga ccaccagggc cgcatgctgc tcatgtaagc
    24841 gccgcaccac ttcaggcaac agagagcttt ccaccgtcga cagcacgccg aggcgcacca
    24901 ccccggcctc atgttcacca ctgcgcaggc tgcgcacgcc ctcgcgcagg gcctgcacgc
    24961 tgggcccggc gtagcgcaga aaggccagcc cggccgcggt cggcacgacg ccctgcttac
    25021 tacggtcgaa caggctggcg gcgagaacct cctccagttc cttgagggtc ttcgacacag
    25081 ccggctggct gatcgccaat acctcggcgg cgcgcgccag gctgccctgg cgtgccactt
    25141 caagaaagca cagcagatga cgaaacttga tgcggttgtc gatattcacg gcgagctcag
    25201 cgcggggaaa gaccccaagc ctaacgccaa gtagcccgcc aagacaaaga agcggttatg
    25261 gatagtggcc aactcaaccg gatgcaaatg agcgctctgg cgccacaaca ggctgacgta
    25321 actctcgccg aagtcatgaa gccacaaacc gggcgtttat cgaacaacct ttaaacgcgc
    25381 tgcagcacct catcgacggc tctccaaggc aggcactgca aggcctgaaa taacaatcac
    25441 tgcgcgccgc cctcttccca gaccttcggt tgcttgtacc aagccgggat tgcacagcaa
    25501 gggcaattga ccaaactaac tgtttagtac attttgatct gtaagacgcc atctgcctcg
    25561 gcgaacgcac aataacaagc acaggtcatt gcaatgaatc acagccttct ggtgctcaac
    25621 ggccctaacc tgaacatgct cggcacgcgt gagccagcga catacggcca cgaaaccctc
    25681 gccgacatcg gccaactgtg ccagcgcgca gccagccagc acgacctaca actggagttt
    25741 cgccagacca accacgaagg cgaattaatt gactggattc accaggcgcg cggccgtttt
    25801 gcaggcatcg tgatcaatcc cggggcctgg acccacacct cggtggcgat ccgcgatgcc
    25861 ctggtggcca gtgaattgcc agttatcgag gtgcacctgt ccaacgtgca caagcgcgaa
    25921 gcgttccgtc atcactcctt tgtttccggc attgcggtcg gcgtgttgtg cggtttgggc
    25981 agcaacggtt atcgcctggc catcgaccac ttcgcccact tgctgaaagc ctgacgccat
    26041 gacccagacc acccagacat ccatccttgc cggcctgatc ggtgccggca tccaggcatc
    26101 gcgcacccct gccctgcatg agcatgaagg cgatgcccag ggcatccgct acctgtatcg
    26161 cctgatcgac ctggacgctc tcaagctgga tatcagcgcg ttaccacagt tgctcaaggg
    26221 cgccgagcag atgaacttca ccggcctcaa tatcaccttc ccctgcaagc aggcgatcat
    26281 cccgctgctt gatgagctgt cggacgaagc ccgcggcatt ggcgcggtca acaccgtggt
    26341 gctcaaggat ggcaagcgca ttggccacaa cactgactgc ctgggctttg ccgagggcct
    26401 gcgtcgcggt ttgggcaatg tagccaagcg tcgtgtggtg cagatgggcg ctggcggtgc
    26461 cggcgctgcg gtggcgcatg ccttgctcag cgaggggatc gagcagctga ctatttttga
    26521 agttgacccc agccgctcgc agagtctggt ggacagcctc aatgcgcatt tcggcaacgg
    26581 ccgtgccgtg gttggcagtg acctggccgg cgcagtggcc gccgccgacg gcctggtcaa
    26641 caccaccccg gtgggcatgg ccaagctccc tggcataccg ctccccgccg agtatctgca
    26701 cgctggcctg tgggtcgctg aaatcatcta cttcccactg gaaaccgagc tgctcaagca
    26761 agcacgcgcc ctcggctgcc gtaccctgga tggcggcacc atggcggtgt tccaggcggt
    26821 caaggcgttc gagttattca gcggcgttgc cgccgatgcc gaacgtatgc aggcgcactt
    26881 tcacagcatg aatggctgag ccgtgcggcc atggcgtcct ccgccatggc tattaacctc
    26941 actcaaaaat ccacgcggct aaaggtagag atcaggcctg caggtagcgc agcaccgact
    27001 cacagatcac ctgcttgtgc cgcgccttga gcgcagcgtc atcgcccagg tcgatctgga
    27061 agatatggct gaaggtatgc cggttggata cgcggtagaa acagaacgag ctgatcaaca
    27121 gatgcacatc cagcgcctgc aatccctggc gaaaggcacc actggcctcg ccacggcgca
    27181 aggtctcgtc gagcagctgc aacacgttat ggctcattga acggatcgcg ctggattgct
    27241 taacgttctc gccattgtgg atgttttcga tgcagacgat gcgcacgaaa tccacattgc
    27301 ggtcatggtg gtcgaaggtg aattcgacca gccgctgcag cgcctcgcgc ggctccagct
    27361 cagccaattg cagcagccct tcggtgcggc ggatatcgcc gtaaagcttc tccagcacct
    27421 cgacatacaa ctgctccttg ctgccgaagt agtaatagat catgcgtttg gaggttttcg
    27481 tgcgctcggc gatggcatcc acacgcgcac cggacaggcc gtggctgacg aactcggaga
    27541 tcgctgcacg gaggatgtcc tctcgggttt tttcagggtt gttcttgcga ctcttgcgcg
    27601 gggtctcgag tgttggctcg agcaattcag tggtcattgt tatgggctca cggccaagta
    27661 ctgatggtgc cgattatggg cgcgacgcca agccgaagga agacacccgc gcgcgccgga
    27721 cagttgctcg cgcaacaatc cggcattcat ccaggcatca gctacgcatt tgctgcgccc
    27781 caccactgcg gctcttggcc atggcggcca ggcgtaccgc gacgttagcg gcgccgtagc
    27841 caatatagcc attcttgcgc tggatgatct cgaagaagaa gcgctcatca aacgcctcgg
    27901 tgtacacatg gaacaactcg ccgccctggg catcgcggtc gtacagcacg ttgtaatagg
    27961 ccagctcgct gaggaactca tcgtcgaagt cgaaacgcgc ggcaaggtca tcgtagtagt
    28021 tcagcgggat atccagcagc ggcacgccgg catccttggc ccggctgacc tcggcaaaga
    28081 tgtccgcaca ggagaaggcg atgtgatgca cacccgaacc gcgataactg gatagcgcat
    28141 gggaaatagc ggtgttgcgg ttctccgaca cattcagcgg cagccttacg ctgctgcagc
    28201 ggctgcggat ggcgcggctc ttcaccagcc cataagggtc cggcagcacc acttcgtcat
    28261 ccgcctcgaa gtccagcagg ctcttgtaga acaacaccca gctatccagg ctatccgccg
    28321 gcaacgccat ggccatatgg tcgatgcgct gcaaaccgcc gcccgccccc gcctgactgt
    28381 cgagctgaaa atcactgtca tagatggtgc tgccaggctc ggcggactcc actagataga
    28441 tcaggctacc gtccggtgca cgcacggcgg gaatttcacg ttcattgggg ccgaccaagc
    28501 cacggtatgg ctggcccttg aaggcacgcg cacgctccaa agccaaactg ctgtccttaa
    28561 cccgtagggc ggtggcacag agtgacgggc cgtgcgcctc gaagaagctg tgggcaaaag
    28621 aatacggctc ggcattcagc acgatcttga tatcgccctg gctaagcagg ctgacggcct
    28681 tggagcggtg cgtgccgctg cgggcaaagc ccaggcgttc cagccagccc gccaacttgg
    28741 caccctgcgc ttcgtccacg gcgaactcga ggaactcgac accatcgtaa cgacccgccg
    28801 gcggtggcgc gaacagcagc tgtggatcgg ctggcagagc tgcctccttc tccaaacgct
    28861 ggcgggtctt ctcttccaga tacagcaagg agcgcaggcc atcggccgca ttagcccgtg
    28921 gcggcgcagc gcggaaaccg tcgttgaaga tttccaatga cagcgggccg ctgtagccgc
    28981 tcttcaggat aggcgcgaga aaacccgcca agtcgaactc gccctgccct gggaagcagc
    29041 ggaaatgccg actccactcc agcacgtcca tggccaggat cggcgcatcg gccatctgca
    29101 caaagaagat cttgtcgccg gggatctcgg caatcccctg agggtcacct ttgagcgaca
    29161 aggtatggaa gctgtccagc agcacgccga gggctgggtg atcggcctgg cgcacgatat
    29221 tccacacctg ttgccaggtg ttcacatgac gcccccaagc caaggcctcg tagccgatgc
    29281 gcaggttacg cgcaccggca cgctcaccga gcaggcgcag gtcatcgatc agaatttgtt
    29341 catcgccaag ggagtcggcc gccacgttgc tgcacaccag caccaggtcg gtgcccagtt
    29401 cctgcatcag gtcgaacttg cgttcggcgc ggtctaggtt cttctccagg cggtcacggc
    29461 gacagccttc gaaatcgcga aatggctgaa acaggctgat ggctagcccc aagtcggcgc
    29521 acatcttgcg cacatcgcgg gggctgcctg cgtagtagag caagtcgttt tcaaaaattt
    29581 ccacaccgtc gaacccggcg gcggcaatgg cttcgagttt ttccggcagg gtaccgctta
    29641 acgatacggt ggcaattgaa cgctgcatgc tgcggctcca gtgggtgaat cggcaccgtg
    29701 gcggcgcttt tagggattcg actgagggta atttgcagta ttggcggggc gctttaggtg
    29761 gggcaagaaa aatgttctaa ctagttagtt ttgtgttcga ttatcgcgca ccactctctt
    29821 ccacttcgac aaatctgcgc cgcaacgaag cgcggttgta gtacaggaat aaaaaagccc
    29881 gtcacaagga cgggctgaag gtttcacaca cgaggagtaa tcgatgccga tggagcggtg
    29941 gatttacaga agggacagcg ggtagttgaa gatcaggcgg ttttcgtcga actcggtgct
    30001 gctgtagtcg cgacggatgc ttgcgttacg ccatttcacg ttgaggtttt tgaacgtgcc
    30061 gctctgcacg gtgtaggcca gctcggactc gcggccccat tccttgccgt cgttggtgtt
    30121 ggccgtatgc acgttgtcgc cgctgacata acggttcatc agggtcaggc cagggatgcc
    30181 gactgcagtg aagttgtagt cgtgacgcac ctgccaggag cgctccttgg cgttgtcgta
    30241 gctggagttg aagctgtcgt tggccagggt gccgccgctg gtgccgttga cgcgcatcca
    30301 gccggtgtcg ccgctgactt tttgcaggcc gacgtagaag gtgttgctac cgatcttggc
    30361 cgagaacagc ccggagtagg tcttgttgtc cagctcgcca gcgcgctcgc taccgtcttc
    30421 gctgccctgg aagtagccga ggttggcgcc gaaggtccag tcgccgaccg gctggctgtg
    30481 cagcagctgc aggtactgct ggttgtagac gtctttcagc tcggcattcc acagaccgac
    30541 cagggtgcgg tcctggttga acttgtactc gccgccggcg aagttgaagc gatccgacaa
    30601 cccgccgccg tagctcatgt cttccatgct ggcgttgttg cgttcgctgt tctggcggaa
    30661 ctggccgccg tagagggtca ggccggcgat ttcattggag gtgatctgcg caccttcaaa
    30721 ggtttgcggc agcgagcgac cgtcgtctga acggaggatc ggcagcaccg ccatccactc
    30781 accgactttc agttcggtct tcgacagctt ggccttagcg gccagggcca ggcggccgta
    30841 gttgtctgcc gggcgaccgt cgtcatgcac cggcagcagg ccggtgttgc gggtgccgcg
    30901 gccgccatcg agtttgatcg cgcccatggc cagtgcatcg aggccgaaac ccacggtgcc
    30961 ctgggtaaag ccggagcgcg cgtcgaggat catgctctgc gtccactctt cggctttgct
    31021 ctggctgttg gccgggtcga cgaagttgcg gttgatataa aagttgcgca ggccgagggt
    31081 gactttggcg tcgtcggtga agccggcggc catggcgccg gatggcagag ccattagcag
    31141 gccgagcgaa gcagtaccaa gcgcgccgaa aaccaggttg ttgtgctttg tcattgttgt
    31201 atacccgttt aatttggacg agcgatgccc tacaaatcct caaaggactc gcatagacaa
    31261 tacgcaagag ttgtacggtt cctgactcaa tgaaccgctc tagataccga gcgatgctta
    31321 atcgcagcgt gaacccggca gtgcacaggt gctggccgtg cccaaaggca cagccggatc
    31381 agctcactga gccgcgcgca ccttggcaat ttcgtcgtac atcatcttca ccagaccggc
    31441 atccaggtct ttggagaatt tctcgatcac cggctgcacg cgctcacgga aacggtcttt
    31501 ctcctcgacg gagatatcgt tgaccgccat ctcggcttcg agtgcggcgc gtgacttatc
    31561 catgctggca gccgtgactt cgcgctgaaa cttctgcgct tcggcggcgg cttcacggat
    31621 cagccccttc tcgtcgtcat tcaggcgact ccaggttttc tggctgatga tcagcgattg
    31681 cgggttgaag atatgcccgg tcaccgacag gtacttctgc acttcgaaga acttgttgcc
    31741 ttcgatcacg gtgaaggggt tttcctggcc gtcgatggtc ttctgctcaa gggcggtata
    31801 cacctcgggg aatgccatcg gcaccgggtt ggcacccagc gcgctgaagg tttccaggta
    31861 aatcggcgac tgaataacgc gaaccttcag gccttgcatg tcttccagct tggtgactgc
    31921 atgtttgctg ttgctcaggt tgcgaaagcc caggtcccag taacccaacc ccaccaggcc
    31981 tttactgtct aactgggcag agagtttttc accgactggt ccgtcgatca ccgcatgcgc
    32041 ttcctcaaca ctgttgaaca ggaaggggaa gtcgagcatg gcgtaatcag cggcttgagc
    32101 agcaagaatg ccggagttga gcacagtgat atccagcgtg ccgccctgca gggccgacac
    32161 cgtctgcaca tcgccgccca atgtgccgcc tgggaacaga cgcaccttga gcttcccgcc
    32221 gctcttctca ctcagcaggt cggcgaactt ctgcgcgccc tggccttgcg ggtgttcttt
    32281 aacgttctgg aaggcgaaac gcagggtgcg ctcgcgaatt tcttccgagt ggctgactgc
    32341 gctgctcagc agcagcccgg tggcgcacgc gccggcaagc aaggttttca tcaattgttg
    32401 catgtcactc tccgatcatt gttgttagag gtcgagttac tacagcgtcc accggtatta
    32461 gccggtgaag aacttcagcg gtccgaggac caattgcggg aacagcacca agagaaacag
    32521 caccgtcagt tgcgcgagta ggaacggcca tacgccgccg acgatttcct cgattttcaa
    32581 ttttgagacg ccactgacta cgttgagcac cgtacccact ggcggagtga tcaagccgat
    32641 tgcggtgttg atcaagaaca gcacgccgaa atacaccgga tcgatacccg cctgggtaat
    32701 tgccggcatc agcacaggcg tcaggatcag gatggtgggc gtcatgtcca tcaccgtacc
    32761 gaccaggatg atcagcagca taatcaccac cagcagcaag gtcggattgt ccataaaggg
    32821 ttcaagaatc gccgccaact ggcctggcag atcggcaatg gtcaccagcc aggacgagac
    32881 catggcggcg gcaaccagta gcatcactac cgaggtggtt ttggccgaag acagaatcac
    32941 ttcgtacagc tggctgactt tcagctcgcg gtagatcacc agcgacacga acagcgaata
    33001 gactgccgcc actaccgccg cttccgtggg ggtaaagatg ccgaatttca ggccaaagat
    33061 gatgatcagc ggcaagccca ttgcccagct gccctcaatc agggcacgca gcacttcggc
    33121 acgcgaacgc ttgggcggcg tttcgacgtt ctcgctgcgc gagataaacc accaggcgat
    33181 ggccagcgcg acaccgagca tcaagcccgg cacgataccg gcaagaaaca gcttggaaat
    33241 cgacaccccg gacgccacgc caaagacgat aaaaccgatg ctcggcggaa tgatcggcgc
    33301 gatgatcccg ccggcagcga tcaggcctgc cgaacgcgcg cggttatgcc cggccaatac
    33361 catcatcggc accagcaagg ccgccaatgc cgccgcatcg gctaccgccg agccagagag
    33421 cgaagccagc agacaggagg cgatgatcgc cacatagcca aggccgccac gcttgtgccc
    33481 aaccaacgcc atggcgatat tgacgatgcg tttggacagg ccgccggcat tcatcacctc
    33541 gccggccagc ataaaaaacg gcacggccat gagcgggaaa ctgtcagctc cgttgagcag
    33601 gttctgcgca acgatctgtg catcgaacag gtccaggtgg agcatcagcg ccacgctgac
    33661 cagcagcaag gcaaacgcaa tcggaatacc cagcgccatg ctgccgagca gcgaaccgag
    33721 gaagataacc agggtcatgg acgggtgctc cctgagttgt tctgctcttg ctggataggc
    33781 tgaatgtctt cagcggcatc gttatccttg accatcacca actcgtcatc accaagcttg
    33841 ccgaacagcg ccaggtagag ttcgtaaaga atgattgcgg cggcgctggc accgaacacc
    33901 aggcccgcgg catagaacag cgccatcgac agaccggaag ccggagccac gacattgagg
    33961 ttgatcactg cctgttgcca gctgccttta aagatcaacc aacagataaa cagcatcagc
    34021 acatgcccga tcaccagaca gatgcgcttg cccaggggcg gcaaacgttt gaccaggcta
    34081 tccaggccca ggtgggcacg gtccttgagg gcaaccaggg caccaagaaa gatcatccag
    34141 acgaagaacc agcgcgacag ctcctcggat acgctgatgc ctgagttgaa ggcataacgc
    34201 agcaccacgt taccgaacac cagcacgatc atcgcgacca tacacagcac aatcagcagc
    34261 tttagcagtt tgaaatacag atcgacgatt cttgtcatta tgggaacctc acaggcatgt
    34321 tgagtgcagc attccacagg gtgcggtaga accagcattg atgctggccg ctatcggctc
    34381 gcaggagtgc cgctagcgct ctaagcctga agcagtgggg aaaaagcgat ctgcggcgac
    34441 ttgaagccat ggctggccac atgacgggtg cagagcagaa tgagggaggc ggcattgaac
    34501 gcctgcatgc tgtacggcat agggtgttcc tctattttta ttgtggccat tggcaatggt
    34561 agccaaggca agtgctgact aaaatgtact aactggttaa tacggtcaat aacccgcacg
    34621 tcactaattc ggcaatatcg agccctggac cagtctccaa aagcgctctg gtaaaccgat
    34681 taagccgttg taaataaaag aaaaatttaa aatatccgac acaacaagag actgtgcgcg
    34741 cagacctagg cgcccattta aagcgtgcga ttatcgatta tttactgcgc atcggcttac
    34801 acaagttcgt cgcgtcctgc gcgcctgatt ccgttacccg caccgaccag acgcggcatc
    34861 gaactcaacg ttgcaaacgg aaggccttgg gcgacagccc gctgccgcgc ttgaagaaac
    34921 gggaaaaata cgccggttcg gaaaagccca ggctgtccga cacctgattg atggtcatgg
    34981 cggtgtacac caggcagcgc ttggcttcca gcagcaggcg ctggttgatc agttgcaggg
    35041 ccgactggcc ggccagacgt cggcacaggg cgttgaggtg cgcggcgctg atgcccagct
    35101 cgctggcgta ctgttcgatc ggccggtgct cgcgaaagtg gctttccagt agccgggtaa
    35161 agcgctgcag atgctcgcgg ccacggtcct gcacctgggt ctcggcatac gccagggcct
    35221 gggaacggcg gccgacccac accagcaagg cgctgatcag cgactgcaac atcagttcgc
    35281 gccccggctg ctgggtgttg tattcctcgg cgatggagtc gaacagggca tccagatagc
    35341 gccgctgctc acccgccggg tagcaggcgg cgcgcgccag cggcgggctg tcgagcaccg
    35401 tgccgagctg ttccaccagt ggcaaggcca ggctcagcac atggccctgg atgtcctggg
    35461 aaaaacgaaa gccatgcacg ctgagcgccg gcaccacctg caatgcgggc tgttgcacgc
    35521 ggttgaccac gccctccact tccagctcgg cgctgccgcc ctgcacatac agcagctgca
    35581 ccagatcgcc atgccgatgg ggtttgatct cccagtcgtg caggcggctg cgctcgggaa
    35641 tcgactcgca gtggatcaga tccggcgtcg gccaggccgc cgtttcaccg tacagcttga
    35701 acaccggcac cgccgcggca gaggctttat gcatggctat gtctgagttg gctgttgtcg
    35761 acatgctcag ccccctgtag gagcgggctt gcccgcgaat cgcggcgcaa tagaaaaaca
    35821 tcgcggccaa ggccgctcct acgggtttcg cagcacccgc cagcacggct ggcaacactt
    35881 aatgggtaga tagccttatg catcgccagt accctcgaaa agtccagaaa gataatcgga
    35941 tagtgccttc aaaggcgcat ccaatccacc aaaaatgcag aggaagcaac caagccccat
    36001 caacaccctg cgccaccaag gacaataaca atgaagaccc aagtcgccat tatcggtgcc
    36061 ggcccctcgg gcctgctgct cggccagctg ctgctcaagg ccggtatcga caacatcatc
    36121 atcgagcgcc agagccccga ctatgtgctc ggccgcatcc gcgccggcgt gctggaacag
    36181 ggcatggtcg acctgttgcg cgaggccggc gtaagcgagc gcatggatca ggaaggcctg
    36241 gtgcatgacg gcttcgaact ggccttcgat ggccgccgcg aacgcatcga cctgcacgcc
    36301 ctcactggcg gcaagacggt gatgatctac ggccagaccg aagtcacccg cgacctgatg
    36361 gccgcgcgcc aggccagcgg cggcatcacc ctgtacaacg tcaccgacgc ccagctgcac
    36421 gagctcaagg gcgaaaaacc ccacgtcacc tttacccata acaacgaaac cgtacgcctg
    36481 gagtgcgagc atatcgccgg ctgcgatggc ttccacggcg tctcgcgcca gtcgatccca
    36541 gctggcgtgc tcaaggaatt cgagcgggtc tacccattcg gctggctcgg cgtgctggcc
    36601 gataccccgc cggtcaatga ggaactgatc tacgccaacc acgaacgcgg cttcgccctg
    36661 tgcagcatgc gctcgccgac acgcacccgt tattacgtgc aggtcggcag cgaggagaag
    36721 gtcgaggact ggtccgataa gcgcttctgg gatgaactga aaagccgcct gccggaagag
    36781 accgccgcac gcctgatcac cggcccgtcg attgagaaaa gtatcgcgcc gctgcgtagc
    36841 tatgtggtcg aaccgatgca gtacggccac ctgttcctgg tcggcgacgc cgcacacatc
    36901 gttccgccca ccggggccaa gggcctcaac ctggccgcca gcgatgtcag caccctgtat
    36961 cgcatactgc tcaaggtcta ccgcgaaggc cgccatgacc tgctggaaaa ctattcgacg
    37021 gtgtgtctca agcgtatctg gaaggccgag cgcttctcct ggtggatgac ctcgatgctg
    37081 cacaaattcc ccggcaccga cgccttcagc cagcgcatgc aacagaccga actggactat
    37141 ttcgtcggct ccgaggccgg gcgcaagacc attgccgaga actacgtcgg cctgccttac
    37201 gaggccatcg agtagctgta gcaaggttgc caaacaaaaa agcccgaacc gttaccggtt
    37261 cgggctttgc ttattcaaaa cgcgaaaagt cagtcctggg cgacaggaaa cgccgtcaac
    37321 ctgaccgccg aggtcatgca ataaatttca gagttgggtt cagctgccaa ggcggcaggg
    37381 ccaacgccat tgaccatatg acgaatccca ccctcaccga aaatcacccc actggtcata
    37441 gacttgaggc gtacgccagg tgtttcaggc gcttcgatgg cactggaggc atgcacaaac
    37501 aaacccagcg cattaacatc attgccattg accactagac cgtgatcggc atgactctga
    37561 ccccaggcag tgcacgtccc atggtcgcct cgtcacaacc aactgtgatc caaaatcaca
    37621 ttattactat taacttcaac gctcgttgta gcttgaccca aacgcggccc gtacaaaaat
    37681 atcgctgagt gtagtttggt catatggcgc cccgacataa ctgttctttt tgcctacttt
    37741 cagcagtaca ttcgaacgta cgggacccgc tatgacgctg ggaccaaagt caggtgtttc
    37801 attcttggcc ttcatcggcg tggcagcaac cggaaagcct atcaacagtg cactcgccat
    37861 acccgcggca gcgaatgcat gtacaagcaa gttagcgttc actttcacct ttctatctat
    37921 atatttattt ttgtagatca agcacacaac aacggcactt cacaagggcg ccaatcaaac
    37981 tacagggtgc ttggcacaca ctcagacaag cgcgaaacca tcaactcttg ccataagata
    38041 aatatcaata attgcaccca aaaaaactca caccactcgt ttactcactg aatttcactt
    38101 gataaaaatg cagattaaat tcggcgcagc aaagttagcg gcgggtgctc agcattacca
    38161 attgtttttc cagctagaag gtatcgccgc acccgatagg ttaatttccc taagtacatc
    38221 aaatgaccca tgcatcgttc tattttatgg atttaaaaat aaataaagtt aagtgggcgc
    38281 taatcgaact ttgtttactc taagcaattg cagaacgcct attactttcc ctaaagtgcg
    38341 gcccgcagcc ttggctgccg acgtgttctt cataagagag cacggcgctt tttctctgcg
    38401 cccaccgctc cgacgtcgct acccgcctcg tctcgcacgg taccgggccc gcgttgcgcg
    38461 ggttcggcgc ggcgctactg catgagccat aacaacaaga aaggaacact gccatgcccg
    38521 tccgcctgct tttcgtcttg ctgttgctac cccacttggc tttcgccagc cctaacgccg
    38581 aacgcttgca agccatccgc agtcaagcgt tcagcacctg cagcagcctg ttagtgcact
    38641 acaaccccaa tcaaaatgac agcgacccgc gccatgccga gcgctatcga caaaccttgc
    38701 agcagttaca gcgattggtg acgctggaaa aagacccgct actgatccag tacgccaacg
    38761 acatgcgtga acatattgaa aaattggagc atcagtcagc caacactcct gagctttacc
    38821 caacctggat aaacccgttg ctggaggccc aatctcgcct cgaccagcag gccagcgtgc
    38881 gctatgccag cgccacccct atcgagccac gccgaaaaac actgcatcag ctggctctgg
    38941 acattgagcg cctgcagttg ctgtaccaaa cgcgcacgtt tggttccctg gccgtatacg
    39001 tgatgcccat ggacgacact acatttgccc aattggacca gcagattctg cagaacttct
    39061 cggcactcaa gcaggactgg ccagagcatg ctgccgagct gaacaaactt ctgcgtaagt
    39121 acgatttcat ccgcccccgc ctgctcaaac atgaacttgt ctgggtacct ggcagcgccg
    39181 cctactacct gggccaggtc acagatggcc tggcgcgcat caacgcagag taaggaaaca
    39241 acgcatgatc cgcaccgccg cctcagtgcc actcacgccc actcagttgc gcagggtgct
    39301 ggccggtctg atgctcggcc tgttcctcgc ggccctcgac caaaccgtgg tagccgtggc
    39361 cctcgcggat atcgccagtg acctgcccgg cagccagctg ttggcctggg tggtatccgc
    39421 ctacctgcta gccatgaccg tcagcacgcc gatctttggc aagcttggcg acctgtttgg
    39481 ccgcaggcgt ctgctggcaa tcgccatcgg ggttttcgta ctggcttcgt tgttctgcgg
    39541 gctggcgcaa tccatgccac aactggtagc tgcgcgagcc ttgcagggga tcggtgcagg
    39601 cggtctgctg gcgatcattc aggccttgat tggcgacctg atcgctccgg cagagcgagg
    39661 tcgttaccag gcctggttca gcggcatgtt tgccctggcc agccttatcg gcccaacctt
    39721 gggtggtctg ctgagcagtc aatggtcatg gcgctgggtg ttctggatca acctaccgct
    39781 cggtgctctg gcctatatcc tcagccaacg ccgcctcgcc ggtttgcagg agaaccgccg
    39841 cgctgcgcgc atcgactact tgggcagtgt tctactgatc atcggcttat gcagcctgct
    39901 gtggctgatc aaccgtctcg gccagcaagc actgtggctg aacgtgcaga atggtttgct
    39961 gctattgctc gcggtagttg ctctgaatct attcatcatc cagcaacggc gcagtctcga
    40021 tccattgatg ccattggctc tacttcgcat cgacgcggtg cgttcaggct ggctattgct
    40081 gttctttgcc agctttcagg ctgtagggct ggcggtactt atccctctgc aggcctacaa
    40141 ctcgggtggg accggagtca gtgcgagcca gttgatggct ctggccctcg gcacaccgct
    40201 gggggctttt ctcggcggcc atctcagtgc ccgcctgggc cgctacaagc caatgatcgt
    40261 tgccggagcc gtactcctgc ctatggcctt gctggttctg agcatgctgc ccagtcccca
    40321 tggcccagca actctgatcg tcttgctgct ctgcggcgca gccatcggcc tgcagttccc
    40381 aacctctctg gtcgccgtgc aaagtgcggc gccccaccat cacctgggtg tggtgacggg
    40441 agtctgtgga ttatttcgag ggctgggagg catgctgggt gtatcactat taaccagttt
    40501 gctgtggcaa ttgctgccgg ggatcagcgc cgactcaatg gcagagacag gcatgaatct
    40561 gtcagcagtg gacagccatc tgttgcgcga ggccttcgcc acgttgctgc agatcgatgc
    40621 ggcaatcacc ctgctgccgt tgttgattgc cctgcgcttg aaagatcgct cactgagccg
    40681 cgaccttact gccgcacctg agtaaatgcc tactccaagc gcgcccgcca ctctaccgtc
    40741 agtgcttcag tacccgccat acgttgcaga tgctcggcaa ccacttgctg catgcgcacc
    40801 tgattctcct ccggcatatg caggttgacc tgcagtacgc ccggctcgac ggcaaactcg
    40861 cacgtgccat tgggaaatgc cacagagccc ttctcctcgc ccaactcgac attgaactta
    40921 tggctccagt gcttgcaaag ccgggtcatc agtcgagctg ggttaacgca ggttacgtag
    40981 gcttcactga ttgtcatacg atgttcctca ttgtggttcg cggtagtaac ccaccgccac
    41041 taggtaatgt ccgactttac gcaacaacgc gtacttgtcc tcgaccttgc cggttaccgg
    41101 atttttccag cggtagtcat agccggcact gtagcttttg ttcatcaggg ccagaatcgg
    41161 tgccccgact ggcttgcctt gcgggtcatt gatggatgcg aaatccacgc cgagaagccg
    41221 caggttgtag ccgtgggcca cgtagcggcg gttatccaga tcgacaacga agacatacag
    41281 atcatcctca cgaaaatccg ctgacagatt gttgattgag ccaagcgtgc cttgcaggtc
    41341 atgctccagc gccgatactg ctcggtccag caggctctgc gcctgttgcg gggtggcgcg
    41401 cggcaggtag tggccaactg cgatgatgcg ttcacccacc cgctggtaat acacctgctt
    41461 gcgctcgacc cggccgtcgc gaacattcat ccatcggtat tcggcgcttt cgacacggtc
    41521 ctcggcgctc gccagggcct gggcaaaagc cgtacgcaac tccggctcca gtaccgcaga
    41581 cacatcgcgc ccgatcagca ccgccgagct gccgccactg gcaagcatgg tgccctgggt
    41641 gtcgacgaca aacacatagt gctggccatc gataaactca ccctgacgac tgaacgcggc
    41701 cagagcggca tcgccctgca gacggtaata ctccaccgca ctttgcagca gacgcttggc
    41761 ctgctcgccc tcggacttat cctgcgcctg gctgactccc accagcgcca acagcagcgt
    41821 tacccctaaa tagaatcgct tcatcacatt caccttgttc actgcgtaag tgagcgttgt
    41881 gccgccggct aagggccgag acgacgaatc gtacgaaagc ccacatgggc ggtagccaga
    41941 tcgccttcct gccgttcccg ggcggcaacc cggtagcgca tgcagtaatt ggcggcgcag
    42001 aggaacgagc cccccttgat cacctgatgc tgctcggctg cgacaggcag caagcgcaac
    42061 gcgctcgggt cgccattggc gtgtccttgc agcgcgccgg aatagcgatc agcggtccat
    42121 tcccagagat tgccgatcat gtcgtagagc ccgtaggcat tggccgcgaa gcagccgacc
    42181 ggggcgcgtc cggcaaagcc gtcctcggcg gtattgatca gtggaaaggc accctgccag
    42241 taatttgccg tgggccggcc ctgatcatcc agcggggtgt cattgagcgg ctcacccgtt
    42301 accccgccac gcgcggcata ttcccactcg gcctcgctcg gtagttggtt gcccaaccac
    42361 tgcgcatagg cccgggcatc ggccaacgtg accaatgcca ctggcagatt gccgggcgtt
    42421 gcatccgcat cacccgggcc gtcaggttga cgccagctgg cacccggtac atagcgccac
    42481 caggaaagcg gggtcgcatt tagctgttcc gcgcttggcg tagcgaaaag cgccccgcca
    42541 ccatcgcgtt cggcttccgt tacataaccc gtggccgcga cgaatgcggc gaattgcgcc
    42601 tgggtcacct cggtgcggtc gatccagaaa tcaccaaccg acactgcgcc cagagggcgc
    42661 tcatcggcat agccgtgata actgccaggg gtaaactcgc cacccttgat ctgctgcata
    42721 ccagccagcg gatcgcctgg ccagcccggc ggtaagcccc tgtaaacgct acaaccctgc
    42781 acactgccca gcgccacctg agcctgctga tagaaccgca agcccagcgc cgcgaaacca
    42841 agcagcaaca ggcttgcacc caccagtagc ccaccacgcc aaaccatcac tgcgcctggg
    42901 caggtatggg cggtacaacc acgcccacct gccgggcata gccttgccag gcctgtttca
    42961 ggcgtgccag ggtatccgct tggctggcgg ccagatcatt gcgctcgccc cgatcagtgg
    43021 ccagatcaaa cagctgccag cgcagcggct gcggcgcatc gacaaaaccg ccctgcgggc
    43081 gcatggccag cagtttcagg ttgccctcgc ggtaatacag gttgccgaac aactcgcccg
    43141 ccagcacgcc ttgttgcccg tgtgcggaag cctggccgga cagcatgggc aacatcgatt
    43201 tgccggttat cgggtgcttg gtctgcccgg catagctgtc gcccgggtcg gcgatagcgg
    43261 ccaaggccag gaaggtcgga gccagatcgt cgacccgcgc gataccagtt tccagtccct
    43321 gacgctgcat gctcttaggc aactgcacga tggccggcac actgatgcca ccttccgccg
    43381 tggtgccctt gaacagccgg aacggtgcgg cactgacctc ggcccagcgc acgccgtaat
    43441 caatctgcga accgcgtcga ccgaggtttt catagctatt gtcagtgttc ggcccggctg
    43501 ggtagtactg tgtatgagcc tcgcctgccg caccgttatc ggacataaac aggatcaggg
    43561 tattgtccag ctgaccactc tgacgcagat ggtcgagcag tcggccgacg ttatggtcga
    43621 ggttgtcgac catcgccgca tagatctcca tcttgcgcgc ttcgaccttg cgctgctcgg
    43681 ggctcagttg ctcccaggtc ggcaacccag gcgtggctgg caacggcgtt gccgactgga
    43741 agtcggctgt gatcaggccg agttttttca tccgctcgat ccgtgcctgg cgcaccgcct
    43801 cgtagccctg gtcatagcgg ccacggtact tgtccaggta ggcatcaggc gcatgcagcg
    43861 gccagtgcgg cgaggtatag gcggcgtagg cgaagaacgg ctgatccgcc tgctggctat
    43921 caagcaggta ggcaatcagc ttgtcggtgt agaaatcggt ggaatagaaa tcctctggca
    43981 actcgaccgc ctggccattc tcccggtagt gcacctgttc aaccttggtg gggttgagcg
    44041 tggccggttt gaagtgcgac gcaccgcctt cgagcatgct gaacgagcgc tgaaaaccgc
    44101 gccgatctgg cccctgctcg ggctccaggc caaggtgcca tttgccaacc atgctggtgc
    44161 tgtaaccacc atccttgagc aattgcgcaa tagaatgcgc ctgctggttg agatagccct
    44221 cataccccgg cttgccacgc tgaaatggct gcaaagcctc ccccatcgtg ccaagcccga
    44281 ccaggtggtt gtcggtacct gacatcagca tcgcacgggt cggcgagcaa gtcggcgctg
    44341 cgtacatgct tgttagctgc aggccgccgg ccgccagttg atccagattc ggtgtgttga
    44401 tttcgccgcc gaaggccccc aggtccgagt agcccaggtc atcagcgacg atcagcagga
    44461 tattcggcct ggcatcagcc gcaacggcat tgctagccgc cagcaacccg agcagcacga
    44521 caggcagcac gttattcagg tgcattggaa cctctctgta gacttaggcg actaccgcgc
    44581 agccggtggc atcacgtagc tcggacaggg aaacgccctc ggccaactcg accagttgca
    44641 ggccctgctc cgttacatcg agcacagcca ggtcggtgat gatccggtcg accaccccaa
    44701 caccggtcag cggcaagtcg cagcgttcta gaattttcgc cgcgccgccc ttggcgcagt
    44761 gctccatcag caccaccacg cgcttgactc cagcgaccag atccatcgcc ccgcccatgc
    44821 ccttgaccat cttgccgggg atcatccagt tggccagatc gccttcggca gacacctgca
    44881 tcgccccgag aatcgacaag ttgatatgac cgccgcgaat catcgcgaag gagtcggcgc
    44941 tgttgaaaaa gctgctgccg gggagcgtgg tgatggtctg tttaccggca ttgatcaaat
    45001 cggcatcgac gtcctctgtt cgtggaaagg ggccgatgcc gagcaggcca ttctcgcttt
    45061 gcagccagac gtccatgccc tcggggatgt agttggccac cagggtcggc aggccgatcc
    45121 ctaagttgac gtagaaacca tcctgcaatt cttgggcggc gcgttgcgcc atctgttcgc
    45181 gggtccaggc catgctcaaa tcctcaccgt gcgttgttcg atgcgttttt ccgggctggt
    45241 gttgagtacc aggcgctgca catagatgcc tggcaagtgg atctggtcgg catccagttc
    45301 gccgatctcg acgatctcct cgacctcggc aatgcacacc cgccccgcca tcgctgccag
    45361 cgggttgaaa ttacgcgcgg tcttgttgaa cagcagattg ccggccttgt cggccttcca
    45421 cgctttgacc agagccacat cggcggtcag cgacttttcc atcacgtacc actggccatc
    45481 gaactggcgg gtttccttgc cttcggccac cagggtgccg tagccggtct tggtgaaaaa
    45541 ggctggaatg cccgcaccgc cagcgcgcag cttctcggcc agggtgccct gcggggtgaa
    45601 ctccagctcc agttcgccgg ccaggtactg gcgctcgaac tccttgttct caccgacgta
    45661 ggaagaaatc atcttgcgga tctgccgggt ttccagtagt tggccaaggc caaagccatc
    45721 gaccccggcg ttgttgctga tcgccgtcag gttgcgcgcg ccgctgtcac gcaaagccgc
    45781 gatcaatgcc tcgggaatcc cgcataggcc aaaaccgccg accgccaaag tcatgccatc
    45841 agcaacctgc ccagccaggg ccgctgcagc gctgggaaat actttatcca cgtcttattc
    45901 ctcaaaaatc gccacggcgc ggacgaaacc ggccgaggcg tgtttgatct tgtaggggaa
    45961 gcacgacacc tggaagccgt tggccggcag gctttccaag ttggccagtt tctccatctg
    46021 gccgtagccg atatcgcgcc cggccttgtg cccttcccag atgatcgagg cgtcgccact
    46081 ggcggcgaag cgttcgcggg tgtacttgaa cggcgcgtcc cagctccagg catcggtacc
    46141 gaccacccgc acaccgcgct ccagcagata aagcgtggcc tcgcggccca tgccgatgcc
    46201 ggcttcgaga taacccggct ggccgaacag tgcgccggca cgggtgttga ccagcacgat
    46261 atccagcggc tgcagcgcat ggccgatgcg cgccagctct gcctctacct cagcggcagt
    46321 gactacatgg ccgtccggca tgtgacggaa atccagcttc acgccgggtt gcaggcacca
    46381 ctccagcggc agttcgtcaa tgccaaaggc cggcttgccg ccatcggtgg tcgatgcgta
    46441 atgccagggt gcatccatat gcgtgccgct atgggtggtg atgttcaggc gctcggcggc
    46501 ccaggactcg tcgcccggta gctggtcctt gctcaggcca gggaacatcg ccgccatctc
    46561 cggccagccc tgctggtggt ccatgtagtc gatcttcggc aacaagggcg gcggatcggt
    46621 gtaggggttg ttgtcgaggg tgacggaaag gtccaccagg cggcgtttag acggttgcat
    46681 gggcagtcac tcctgcgctg cgcgaacgtc gcgccgcaga caaagggtta agggaattgc
    46741 gcaccagggc cgcggccgaa ccgtcgtggc gaaagccaag ggcgcgggcc tgcggggttt
    46801 tcattgccgg agcgctgccg aacagagcct gcaggccggc atccggggcg aagctgatca
    46861 gcgcccgacg ctcctggccg taggcgttgg ccagagcatc gagtacctgg gcgatggaca
    46921 gttgcagcag cggcaattgc cacacgcgcg ctgggccgag gctgctgccg tccagctcgg
    46981 cggcatgaat caggttgtcc acgcagcaac gtagcgacat ccaccagcag cttgcctctg
    47041 ccgataccgg gcagcgatag ggctcgccag cggcaaaggc ttgcagcagg tcgctcataa
    47101 aggccgagag cagaccattg ggttcacgcg gacgggcgac gatgcccggc agacgcagcg
    47161 cgcggccgtc cacctcgcca cgccgggcca ggtcgttgag cgcggtttcc accatcttct
    47221 tgtgcgcgcc gtagctcagc tcggggcgca gcgcggcatt ttcatccatt cgcgccggca
    47281 gcgcgccgcc atacaccgcc acgctgctgg catacaccag caccggcgcg ccgggctgac
    47341 tgcgcagttg gtcaagcagc tccaggctgg ccagcaggtt gacctggtag ccgagggcgt
    47401 actgctcctc ggcggcgcca ccgggaatgc tcaccagatg gaacaccacg tcgatgccat
    47461 cggccagcac acggcgcagc aaggcggcgt cggtgacgct gccatggtgg cgacgcaggc
    47521 gattgtcctc gggaaaaccc accaggtcct tgtccagcag catcagcgca gagatcggct
    47581 gaccgcgcag ctcacccgcg tcgagcaaac gctgcaccag cgcccgcccg acgaaaccat
    47641 tggcccctgt gaccatcacg cgcatggcgt tctcctcagc cgacggccgc tacgacacgt
    47701 tgatcgatgc cgccgaacag cgactgcccg tccgcatcca gcacttccat gcgcactcgg
    47761 tcgccgaagc gcataaagcc ggtctgcggc gcgccgtgct cgatcatctc gatggcgcgg
    47821 cgctcggcga tgcaggccga gccgacactg cgctcggcat tcgacacagt gccggaaccg
    47881 atcagcgtgc cagcgcctag gcggcgggtc agcgcggcat gggcaatcag ttgcgggaag
    47941 ctgaagttca tcgccccgcc attggggttg ccgaaccact taccattcca ttccacctgt
    48001 agcggtaggt gcacgcggcc atcgcgccag gcatcaccca gctcgtccgg ggtcaccgcc
    48061 acagcagcga agctggacga cggcttggcc tgcagaaagc cgaaaccggt cttcatctcg
    48121 cgcggcgcca gggcgcgcag gctgacgtca ttgatctgca ggatcagctt gatatgccca
    48181 cctgcctgtt cggccgggca gcccagcggc acctcatcga ccagcactac gaactcgcct
    48241 tcgaaatcga tgccctggct ctcgctgggt agccgaatgt cgtcgcggcc accgagaaaa
    48301 tcgtcgccag cgccctggta catcagtggc gtggtctcag ctccatcgat tggatcgaga
    48361 tgaaaggcct tctgcatcag ctcgccatgg ttgagaaagg ccgagccatc cagccactgg
    48421 taagcgcgcg gcagcggtgc catgacctca gccgggtcaa aggcaaaggt tccctcggcg
    48481 cgaccttcat taagctcgcg gtacagcgcc tgcaattcag gctcgacgct ccaccagttt
    48541 tcgatggcca gttgcagggt ggcggcaata tgcccagcat ctagcgccag gctgaggtca
    48601 cgcgagacca gtagtaggcg gccgtcgcgg ctgccgtctt tcagggtggc caacttcatg
    48661 aacgtactcc gtgcaaggct gcaaggtcgg cggcgtactg gccgtcgaga agaatgaaga
    48721 ccatccgcgc catctgttcg gtgcggttgc tccaggcatg attggtgccg cgttgcacga
    48781 cgatatcgcc gcgcttgaga tgcacgtctt cgctgtccag caccagccac acctcgccct
    48841 cggtgaccac gccgtagtcg agggtttcgg tgcggtgcat cagcttgtgc ttggaatcgg
    48901 cctggccggt gccagcgtgg gcctggccga tttcggcgaa ggcggcaacc gcgtcggccg
    48961 cgctgacctg gttctgcacg ctgtccggtg ggatatccac cacgcggatc acgctgccca
    49021 gcggcatcgg gctcagttgc aggggctggt tggtagggtc atcgccgtta tccagcacgg
    49081 ccgggctggc tttgctgttc cacacctcgt agaacaccgt gccgggcacg gccttaaggg
    49141 ggaagttgtt tggcgttgct ccgctcgtag cgaccacggc caggccatga gcatcgtggc
    49201 cggtgaccac gcgtttgaag ccgggaagtt gttgcatgga tggctcctca aagaattatc
    49261 gtcagctttc ggtcagagag aactcgcgtt actcacacca gcaaacaggc ttgctcaaac
    49321 gacaggcgcg gcccggatgg acgcaacttg ctcacgtcgc catagccgag gctgcagaga
    49381 aaattgcttt tccagcgacc gtcagcgaag aacgtggcat ccaccagggc ggtgtcgaaa
    49441 ccggacatcg gcccgcaatc cagccccagc gcacgagcgg cgagcatcag ataggcacat
    49501 tgtaggctgc cgttacgcag ggccgcagtg tgcgcggcct gcggattgtc gcggtacggc
    49561 gcgccggcgt cgtagatcgg gaacagttcg gggaggtact cgaagaactg gctgtcgaag
    49621 gcgatgatgg cggtcaccgg ggcctgcagc atcttctcgc cgttatggcc cttgagcgcc
    49681 ggcagcaact gctgcttggc ctcagcgctg cgcacgaaga cgaagcgcgc cgggcagcaa
    49741 ttgttgctgg tcggaccctg tttgagggtg tcgtagagct gcttgagcag ctcatccggc
    49801 accgactgtt cgagccaggc attgtgcgag cgggcctcca ggaacagctg agcaatggcg
    49861 gcagcgggaa gcgactgatt cataaaaaag cctcagagat cgagaatccg ccgcttaccc
    49921 atggatggcg agcggctggc aaatgcaaag gaaataagcc gtcggtcatc gtcctagccc
    49981 tgcgccggca gttcagtggc aggcgcaaca ggtgcctgat cagcccgttt gggcagtgcc
    50041 aggcaggcca cggcaaccag cagttgcagg gccgcgataa tccccagcgc ggttggcaag
    50101 tcgctgttca ccccaccggt gatggccagc accaccagcg ggctgacgaa ctcaccggca
    50161 aagaacgccg ccgtaaagaa acccgcagca cgcccacgct gttgaaagcc caccagggac
    50221 atgatccagg tgatcagggt cggcatcatc agaccaatgc ccaggccgtt gatcagtacc
    50281 gcgaccacca ccagggcatg gctgtcggcc acagccatca gcgcaccgcc cacaccggcc
    50341 agcgcaaagg ccagaaacat ctgtcggtgc cccggcatag cgctgagcag gcggaaactc
    50401 agggcaccgg caagcacgcc gagttgactg gcacccatgg tcatgccgat ctgttgcggc
    50461 gcatcgacgt gcagcaggtt gagcaggaaa ccagcttgta ccggcaccac gaacaggctc
    50521 agacccgcca ggaatgacaa ggcatataaa ggtgtcaggg cacgccaggg gaatgcacag
    50581 gtggccacct cagaggcagc ggcagcttgg ccgcgcggct cccagagaaa ccaggccatc
    50641 agcggcagga acaacaagcc gatgccatac agggcaaagg gcgtacgcca gccgtgctca
    50701 ccgagcatgc cgccgactgc gataaagatc gctgccgaca gcgaggtggc aaccatctgc
    50761 agagcgaaca gccgttcacg tcgcgcgccg ttgtagtaat cgcccatcag cgtagtgcag
    50821 caggtcatga tcgccgcctc ggccaggccg ataccggcgc ggctgatcac gatggcctcc
    50881 agcgactcca gccacagcgg caacacgcca cagatggcat agagcaacat ggccccgagc
    50941 agcaacggct tgcgcccgag gcgatcggcg ataaccccgg ccaagggtgc cagcagggca
    51001 atcatcaggg ccggcagggt cagggcgata ggcaccagca cggccacacc cggcgtatcg
    51061 ataaagtgtt cctgcatacg cggcaacacc ggtgccagta gcaccgcccc aagcacgggc
    51121 aggcagctgc cgagcaacag cagcagcgat tgcggcaagc ccgcttggcg ttcagcgccc
    51181 atcacacacc tccagcaacg gaacgcccga aaacgctaca gcgtaggcag tcagcccagt
    51241 cgaaagacca agcaacaggc agtgaaaaca ggtgtgcaaa cggggcatgg caaagtcctc
    51301 tcttgttgtt agggtgcggc cggccaggtg tggccggcta ggtgcggccg agttaacggc
    51361 cgtacgtacc gtcgatctgt ggaccagccg cttctatggg cagcatcagg tgcgtaacag
    51421 caccctcctt ggctgctcgc ggagcgttgt ctttgagctc gtcagaggcg ctcttgggac
    51481 gaaccggaaa caggaagtaa gcgagtgctg gcagcaacaa cagcgcaccg accatgttcc
    51541 aaacgaacat aaaagccagc agcacgccca tgtcggcctg gaacttgatg ggtgaaaaca
    51601 gccaggtgat gacaccaata gccagagtga cgccagtgag aatcaccacc ttgccagtgg
    51661 acaccagagc ccggtaatac gcctcggaca aactggcacc ctggcgctga taaaccagca
    51721 gcatgctcat cacatacagc gcgtagtcga taccgatgcc cacaccgagg gcgatcaccg
    51781 gcaaggtggc aaccttgacg ccgatcccca accagaccat cagcgcctca cagaggatcg
    51841 aggtgagtat cagcggcaca atggcgcaga gggttgcgcg ccaggagcgg aaggtgatca
    51901 ggcacagcag gatgaccgca ccgtagaccc agaacagcat ttcgcggttg gcctgcttga
    51961 ccacgatatt ggtcgcggcc tcgatgccag cattaccggc tgcgagcatg aactgcacct
    52021 cgtcggtgtt attcgctgcg gcgaactgcg cgacgtgatc aaccagccct gtcagggttt
    52081 cagccttgtg gtctgtcagg aagacataga gcgtcagcag gctgcacgac tcgttataca
    52141 acccacgcgg agcgctggcg gtgatcatat tgagcatcgc ctggttgtgc tgcagctcgt
    52201 accacttggg gttaccttcg ctcaagccca ccaacatgcg gcgattgagc aatgccaggg
    52261 agttggtcga atccacacct ggcagggtgc gcagttgcca atccagcgca tcgaccttgc
    52321 gcagcacgtc ataactggca catacacccg acggcgtacg caccatgatg gcgaacagat
    52381 cgctactggc accgtaggcg cggttcataa aggcatcgtc gaggttgtaa cgtgagtctg
    52441 cgcgcagctc gggagcaccg gcatccaagt cgccgatctt caggttttgc gccaccagat
    52501 aaccgccaat ggccaacccc gcggccacaa gtacgcaaat tgtggcgccg cgcttttggg
    52561 tgaacagatc aagcgttcgc cacagcggat ggcgctccat gcgtccaccc tcttcctgct
    52621 cgtcacgcaa gctgcgccgg gctgctgcag ggctgacacc aaggtaggaa agtagaatcg
    52681 gcaacagaat cagattggta ataatcagca cggctacgcc aatgctggca gccacagcca
    52741 agtcttgaat taccggaata tcgataaaca tcagcacagc aaaaccgact gcgtcgctga
    52801 gcaatgccgt caaacccgcg ataaatagac ggcggaaggt attacgggcc gcaacgatac
    52861 ggtgcatacc gcgaccgatg tcctgcatga tgccgttcat cttctgtgcg ccatgactca
    52921 tgccaatggc aaacaccaaa aacggtacca gtaccgagta cggatccaat tcatagccga
    52981 gcagtggcag caggcccaac tgccaaacca ccgcaaccag cgagcagaac accaccagca
    53041 tggtgctgcg cacgcagcgg gtgtaccaga agagcaccgc ggtggtgatc agaatggcca
    53101 cggcaaagaa cagcagaatc tgtttcaagc cttcgatcag atcgccgact tttttagcga
    53161 aaccggtgat gcgaatctcg atgctgtcgc tctggtactg gctgcgcagc gactccagct
    53221 gctgcgacag agtcgagtag ttgagcgcct gaccatcagg ggttttctgt agcagtggcg
    53281 cataaataat gctagacgtc tggtcaaagg ccaccagttg gccaatctcg ttggactgct
    53341 gcacattgcg cttcaacgca ccaatgctcg ctggactggc gtcgtagtca tcgggaatca
    53401 ccgggccacc atccaggccc tcctcggtca ccgccaccca acgggtagct ggggtccaca
    53461 gcgacttcat ataggcgcgg tcgacaccag gaagcagata aatcttgtcg ctcaactcct
    53521 gcagcgtttt caagtatgtc gcgtcgtaga tatcgccctg cttgttgacc accgcgatac
    53581 gcagcgcatt acccagcccg ctcagctcct tctggttatc caggtagttg gcgatatacg
    53641 ggtggttggt cgggatcatc ttctcgaagc tggcattcaa ctcgatacga gtggactgga
    53701 aaccgagaaa cagcgtcgcc gccaggcaca gcagcagcac ccacagacga tgattaaaca
    53761 gcgcacgctc gactaccgag ccggaacgcg gatcaaagtt gtcgaggctg tccgctgggg
    53821 acgagatgga accagtagag gacgtcataa cctcactccg aagcgacggt ggctggctgc
    53881 ggcacgcgca gcaggccggt gaaaccggcg acgatcaaac tgccatcagc ggcctgaacc
    53941 aggctggaaa cgggcacgcc caatggtttg ctaaagggct gcaacgcttc acccttgctg
    54001 agaaagagca tgccggcctg gttggccagc aatacctgac ccgtactcag gcgagtggcg
    54061 tcgctgaagg acaccggcat aggcaccggc gcacgcgcaa agctggcccc ggcatcctga
    54121 gtaataaagg cgtggccttt cagcccgccg accagcagag cgccatcggc gcggctctgc
    54181 agagtgaaga aactgccctc gtagggactt tccagttgcg caaacgaagc accgtcgtca
    54241 tcggagcggg ccagatagcc ctgctccccg gcgagaaacc agcgtgaacc ctgacgactg
    54301 atggcataca ggtgcatgcc catcgggttg gcgatatgcc ccaccacgga atgccagttg
    54361 gcaccgccat cgcgagtttg cagtgccaga ccataggcac cgacgatcaa accctgttgt
    54421 gcatcgacaa actcaagggc cagaaacggc ttatcggcac catctgccac caatcgttca
    54481 gcactttgta cacggacttc ggcactgtcc tgatcgagtg cggtgacctg ttcggccttg
    54541 gctgcatcca gctcgatctg cgcggcgcgc aggccgtcca gctgaacaac ccagctttca
    54601 ccgccatcac gggtggccaa caccacaccc gcgtgaccaa ttgcccagcc attgcgcgca
    54661 tcgacaaact gcacagcact caggctgacc gacaccggga ccacggcttg ccgccaggtt
    54721 tgtccgttgt catcggaaag cagcaccagg ccgcgctcgc ccaccgcgac cagacgctca
    54781 ccggcacgcg ccagatcgat cagtacagtc ttctgagtcg gtgcaacctg cattgccggg
    54841 gtatccagcg gcaaacccac ggggcccgta tccgctgcgc accaggctgt gctgaagggc
    54901 aaggccaaag ctaaaaacca atagcccgcc tgactgattc tgttcattac ccacctcgga
    54961 aaaccaccgc aatagaaggg gcgacatggg cgtcgaccct ccacttcgcc ttgcttctta
    55021 gcgcacgcca cgtcctgcca tggccgccgg ggaaaactcc gaggccttgt agcgatcaac
    55081 cgcgccgtac tggctgtcct taccggtgta gaggttctgg ataaaccagg caccggaagt
    55141 caggtcatag aaacccgctg acaggctggc gtgagctggc aggtcaggca gtactgcaga
    55201 taacgaccac agggttttgg ccagctggcc attgccatcc cagcggtcgc cgagagcggc
    55261 ttgccaggtg tcttcgtcga ggtagtaacg gcccttaggt agttgatgac gcttgcccgg
    55321 caccaggtca gcttccacca cccagacgcg gtgcagttcc cagcgcacgt aatccggatt
    55381 caggtgatgc ttaccgaaca gatcttccgg cttggcggca gcctgtacct tgttggtgtt
    55441 gtaggggatg tagacttcct tcttacccac caacttccag ttgaagcggt ccatacgccc
    55501 ctggaacacg ctgagttcgt cgaacgacat cacaccggcg gtggccggag tcggcgtatc
    55561 gcagcaggcg ttaggcagct tgcgcacacg gcgctggccg ctcagataaa cgtgcgcctg
    55621 agacttgtcg ccattgacgt tggtgcggcc catgatctgc tcgccggcac ggatcggtgg
    55681 gccgacgtta agcaggcgga acagccagta atcaccctcg aaggtttccg gcgtgccgtc
    55741 ttcgtagtag tacggcattt cctgaatggc ctggccgtcg gtactcatca cctggctgcc
    55801 atcagcggtc atcaggtagt ggcggaagtt catcgacagc gaggtaccac gccagttgag
    55861 cgcgtggttc cacatggctt cagcgccgtt ctgcggaatc gggaacggaa taccgccata
    55921 ggcaccctgt ggcaccggcc cggcgctgct atccaccagt gaagcgcggg tggcattttt
    55981 cagggtgttg tcatacaccc actgcggcgc agcggcggta cggcgggtct ggtaaatgtc
    56041 taggcgatag gtgtccgggt agcgcttgaa catctccttg gtgccatcgc tgagtttgtc
    56101 ggcgtactgc gccatgttct gcgcggtgat gctaaacagt ggcttgtcgg cagcgaacgg
    56161 atcgctgcgc ttgccgcccg gcttgtaggc cggatcgacg gtggtgtagc cgccgtccca
    56221 ggccgggatg gtgccgtcgg cgttaccggc tttttcggca ccgaaaggcg tcagagtttt
    56281 acccaacgct gaagcttgtt cggcgctggc ggccaaggtg attggcgcaa caaccgccaa
    56341 aacggccaag cacaaggcct tgagagtgaa ctgagtggtc atgtgcaagc cctcttaaaa
    56401 cgtggtcttg atcgagaaag ccaggtagtc ccggtctttc aacgactgct tgaagttgaa
    56461 ttggttagcc gaattgagcg tggtgtcctc ggcgccatag aaatgcgtgt aggtcagccc
    56521 cagggtcacg cggtccaggt aggtggcggt cacgccaatg ttcatgtcgc cgccgttatc
    56581 cggcccgaac gcgccaatcg cagacgactt gcccaggggg aaatagctga cacccaccgg
    56641 cacgctgaca tccacgccgg ggaacagctg gcggtaggtt ggcgagtaca ccaacttaat
    56701 acccaggccg tcgtcggtgg cattagggtc gagggcgttg cgattcttcg tgacgttgag
    56761 tacacggttc caagcaatct cgcctaccag gctggcttcg ttggaaataa atgacgggcc
    56821 gagagaagcc agcacgttga ggttgacgtg agccgtgcgg ccgacggcgt acagcggatt
    56881 ctcgtcgtta tccgcgtcca cacctggcaa cacggtctgg ccgttggaga ccagcggcat
    56941 gttccagcgc atcgacgctt cacccgcgaa actatattcg tcgatggtgg tggcgaagct
    57001 ggcacccagc gcgcgaatct cctctgggta aacccaatag aactcaccaa tttggccggt
    57061 gctgaaatta ggtgcacccg aacctggctt cagatacagc ttggggctct tgtcgtggta
    57121 ttgaatggcg tacaggccgt actccgctgt ctccgccgcg tagcgcagtt gcagaccgcc
    57181 ctgaccggag ctcttggctt ttttgtcttt gccgtggaag aacgcggcgg ggctattcgg
    57241 gttaccgccg aggaaggctg ggaatggtgc cccaacgacc agccgctcat taccctcgcc
    57301 aatggtgtcg cttccggaga agtaactgcc aacacccggc aggcgggttt cctcccattc
    57361 aaactgatag aacgcaccca gcgacaggtc atcggtcagc tggtaggtgg tcgacagctg
    57421 gctgaccgga cgggtgattt ccttgaactg ggtgttgggt accgattgcg ccttgaccac
    57481 atccaccggt gccatgccac cagcgatacc gttggcaccg aagaacaagc tctcgcccca
    57541 gatcaggcca tgccggccag ctcgtacgga tagggcgcga tcagccaact caccgttcca
    57601 ataaacgaac gcatcgagca gttcaacatc gccaccgtgc agctgacggg tgtcgtcggt
    57661 gaactgatca aaagccacgg agcgctggtt ggagcgcgcc gggttatcgt tggcgttgtc
    57721 atcctggtac accgtgtcgt accagccggc agcactgacg cgcgcgccga agtcgcggta
    57781 gccgacatcc agctcggaaa ggatatccag acgattggag atcaggccct tgtcgaagtt
    57841 gcgatcacca tcatcctggt ttctcgccac agcactttcg ctgagcttgt cgctcgggtc
    57901 ctgcaggcgc caggccgcgc tgtacttcag ggtattgtcc cagcgcaggc tgaggtctgg
    57961 gttgcccgta tcaatctgaa acgcctgggc catattggct ccaagcacca ggctggccgc
    58021 cagggtcaga gtgaagggtg cacgccgcga cgcggcgatc ttgcgagtag ccatcgaagt
    58081 aaccctcttt attgttcttg tgtgtcagtt cttcttgcgt tttcttgcgt aaccactccc
    58141 ctaccggcca aggcaagcca gtacaggagc aggtattccg gttatccagg ggtgcgcgga
    58201 accagcagca cgcagggtgc cgcgcacgaa ctcagatggg ttcggccgtg agcttgagca
    58261 tctgtttgac caagccgatg cggtcgaagc tgtggtcgtg ggcgatttcc ttgtcacccg
    58321 ccagaatcga actctcggcg atgtacttgc agcgctcgta acggcgcgcc ataaagcgtt
    58381 cgagttgctg ggtgacgctg ccgccggcgg tcaattcttc gccgagcacc acggcatctt
    58441 cgatggccat accggccccc tgccccaggt gcggggtggc ggcatgggcc gcgtcgccaa
    58501 tcagcagcac acggccgcgg taccagggct cgctgacgaa caccacttcc agcggtttgt
    58561 agaccacctg ggcgctgtca gtgatctgct cgcgcagctc gccgatcaga ccgccgaaac
    58621 cactcaggcg cttgcgcatc tcggcggcca ggttggcgct gtccatgcgc ggattgcccg
    58681 gctcgtggga ggtgacgaac atgtacataa ggttgtcggc caacggcacc agaccagcgt
    58741 tgccggcagg gccctggaag ttggccagat gatcgatcgc cgccgggcgc gggaagttgt
    58801 agcgccacac cgactggccg gtgaaacgcg gctggtactt gtcgccgaac agcaggccac
    58861 gcaccttgga gaacaggcca tcggcaccga ccaccaggtc atagcggtcg cggctgccat
    58921 cagtgaacag cacatcaacg ccctcagcat cctcgctcag agtttccacg ctgaggccca
    58981 ggcgtaccga agcaccgagg ccaacagtag tttcgctgag gacgtcatgc agggccagac
    59041 gcgagatacc cacgttggcc gggtactcgg gaccggccag gcgctgacca ggaatacgca
    59101 ccagcggctg gccgtcggtg gtgttgatgc tcacatcttc gaaggcgaag gcggcgtcca
    59161 gatagccatc gagcacgccc agcttggcca tctcacggac cacgttgctc tgctggatga
    59221 tgccgacgcc gtacaccgtc cactcggctt tgagctcgac cagatcgacc tcgataccct
    59281 tgcgacgcag ggcgatggcg ctgcacaggc caccgatacc accacctacg acaagaacct
    59341 tgttcactgc attcatggct gtctcactct tattcttgtg attcgggcta gcgttccgct
    59401 agtccaactg ctgccgcgtg atgttcactt tcccggcaaa acaggggttt gactaatcgc
    59461 ttgtagggat gcgatgtatc gccgttcgcg atacctcacc taccctgcac acagcgccaa
    59521 ctgaccggcc tccagcacca gccagccagc gtccagatgc cagcgcagtg cgctcaggtg
    59581 ctcgcccagg caaggcccgt aaacgcacag accgctttcc ggcaagaact gcgcgccatg
    59641 ggcgtagcag acgatgcgac tgccatcggc cgagagaaaa cggtccttgc ggtactccag
    59701 acgcacatcc agatgtgggc agctattgcg gtaaacccgc acttcgccgc cgtggcgcag
    59761 ggcaaacagg ctgtctttcc ccgcgtgtaa tggatcaaag ccacgggcct gcccctcgac
    59821 cagttcatcc agccggcaca acgcgatagc ggcatgactc acgaggcacc ccgtgctcag
    59881 gcctgcttcg gcggcggacc gcccggagcc cacttcgcct tggaggtgaa caggaacagt
    59941 tgcgagttat ccgcattgag cggggcgaca cgcgcctccc actcatcgtc atgcttgtcc
    60001 atgtcggcgt cgtactcgac gtggcaaccc agcgggctgt tgaagtacca gaaccagttg
    60061 gaaccaaaga gatgacggcc cgggccccag aagctggtcc agcccttctc ctcgaagcgc
    60121 cgcccggcca gtagcacttc ggtgccgcta cccatatgga aggtgaagtg ctcgcagccc
    60181 ttcatatgcg gcggggtctg gatcataaac aggcagtggt ggtcgtcgga cccagccggg
    60241 cgcataaacg gcccggcacc gataaaggtg tcggtagtga tgaagcccag acgctggtaa
    60301 aaggcctcgg ccttggccgc atccgggacg aaatacacca catgcgacag ggtgcgcggc
    60361 agcgccgaca tatccggggt gatgcccggt tgattgagcg ggcgctgcgg cgcgtggccc
    60421 ggcgcgttgg tcagatcggc cggggcgctg aacgcacgtc gcacgctgac ctggaaggcg
    60481 atggcaaagc ccatgtcgtc gacgctatgc aacacgccat tggcgtcacg ggtgaccggg
    60541 cgatcacgcc ccagctcgtc ggcaatggca tccaggtcag cggcgcaggc cacgccatag
    60601 acggtttcgc gaatcgacgg tgccgggccg atggcggccg gcaggctggc atcgtcggcg
    60661 ctgcggataa ttaccgcagt gccgtcgagg gcctcgaagc gcccgccccg gctgcccaca
    60721 tcgaccggct gcaggccata gtcacggagg aaatcgctac aggcttgaat gtcatcgacg
    60781 ccaaagatca gggcgtcaag gccaatgatg ttcatgctta ccactccatt gaaattattc
    60841 tgcggacacc ctactagcgc gacgacaagc gcctggcgga gcaacacggg tatccggtgg
    60901 tggagtcggt gcaagcgtca cggctaaccg actggcggcc agattgccgc gttggtaagt
    60961 cgctgactaa tcgcttgttg ggatgcgacc tatcggcaaa cgcgatacct gaagaggaca
    61021 agcgcaaggg cgagaagtgg gcgattaatg ctcagtccgg ggttgacggc gtaatggatc
    61081 ggcaggaagg tatctcgaaa ataaatacaa gatgcgagac tgccatgcgc ttcaaccacc
    61141 tggatctcaa cctcctggtg gcgctcgatg tgctgcttga agagcagaac atcacccgcg
    61201 ccgccgaacg cctgcacatg actcaatccg ccaccagtgg cgtactcggc cgcctgcgta
    61261 gctacttcga agacgacctg ctggttcagg tcgggcgcaa gatgcagccc accccttatg
    61321 ccctggaact ggtcaccccg gtacgcgaag tgctgctgac catccagtcg tcgatcaccg
    61381 ccaagccggt gttcgacccg accagcagca agcgccattt ccgcatggtg acctcggact
    61441 atctgatcac cgtgctgctg gcccgagtca tccagcggat ccaccaggaa gccccgcaca
    61501 tcaccttcga gatgctcgcc cccagcgaca actccggcga gctgctgata cgcggcgagg
    61561 tcgatctgat gatcgtgccc gagcgctaca tcctcgaagg ccatccttct cagctgctgt
    61621 tcgaggagga acacgtctgt gtggtgtggg atgccaaccc gctggtcggc gaccacctga
    61681 ccctggagca gtacatggac atgggccatg tctcggtagg tttcggccgc acccggcata
    61741 tgagcatcga ggactggttt atgaaccagt acggcttcaa ccgccgcatt gaggtgatca
    61801 ccaacgactt caacaccctg ccccagctgg tcgtcggcac ccagcgcatc gccaccatgc
    61861 accagcgcct ggcccgtctg tacgctgcgc acctgccgct gcgtatcctc cagccgccgg
    61921 tgaagatccc ggtgatgcgc gaattcatgc tctggcaccg caccatggat cgcgacccga
    61981 tgcacagctg gctgcgcgaa cgaatcagtg aatttatcaa cgacctggag cagcactgaa
    62041 caaactggcg cggcacctcc cgacaacagc gctgcgccgg tttaccatct cgtttcgcga
    62101 taccttgcat cccgactcac gattggccaa atagctggcc cctccttaac gtgtccggca
    62161 aacggcgctc gtgggctttg cctgcccgca tcacgacgcc tacccgccac cacgaaaagg
    62221 acaataagaa tgttccgtta ctttcccacc aattacgtct gggacctttc cgtcaatctg
    62281 gccatcgaga tgggtgcccg catcggcgaa atcgaagaga tgtgcaagcc actggtcgaa
    62341 gccgccaagc agcccgatgc cgccggcacc gccgccttcc gcgaaacctg ggcgaagatg
    62401 gccgacaagc tgtgcggcct ggccgaagaa gacgaagccg ccggacgcct gttttccgct
    62461 ggcgagaaat acagccgcgc ctccacctat tacctcacct gcgagcgctt gcaggcccac
    62521 ggcgcgccag gtcgcgaggc cctgtatcag cgcgtactgc agaccttcgc ccgcgggatt
    62581 gagctgtcca aagaaaactg cgaacgcgtg gaaatcccct acgagggcgc gcatatttcc
    62641 ggtctgctgg tacgtgccga aggcgttaca ggtccagcgc cgatcctgct gcaggtcaac
    62701 ggcctcgatt cgactaagga aatgaaatac cgcgtcggcc tacctgcctg gctggccaag
    62761 cgcggtgtgt cctcgctaat catcgatcag cctggcactg gcgaagccct gcgtctgcac
    62821 aacctgactg cccgctacga tagcgagcac tgggccagcc gcgtagtcga ctggctggaa
    62881 acccgtagcg atgtcgatgc caaacgcatc ggcatggagg gcgtgtccct gggcggctac
    62941 tactgcccgc gcgccgttgc cttcgagccg cgttttgcct gtggtgtggt ttggggtgcc
    63001 aaccacgact ggcgcgacgt gcagaagcgc cgcctggaaa aagaaggtga gctgccagta
    63061 ccgcattact ggactcatgt gcgctgggtc tggggtgcca aggatatgga tgagttcatg
    63121 accatcgccg aaaacgtgca cctggacggc gtgctggacc gcatcaaggt gccattccta
    63181 gtcacccacg gctcgaagga ctcgcagatc ccgttaaagt gggccgaacg cacctacgaa
    63241 caactggtca acagccccaa gcgcgaactg aagatattca ccgagcgtga aggcggcgtg
    63301 cagcactcca gcttcgacaa cagcatcaac gccggccagt acattgccga ctgggtagcc
    63361 gaaaatctgg gtggccgtac tgcctgatta accgctgggt caaagaaaaa acgccgaacc
    63421 tgttcggcgt tttttgcatt caggagcatg gttttcatgc ctggcgatgt accagcactg
    63481 tgttgcatca attttcggca gttaagtgag accttgtagg gtggataacg ctttgcttat
    63541 ccaccattag ccctctcgag cctacaccag agcagaggta cgccgtgggc aacaagagtc
    63601 tgcagccgcc gcggtgcgca cggcgcaccc tacgaagtct gcgcggcctc cgctctggtg
    63661 tagggtggaa aactgcgcag catttcccac cgctgggctg gaggtgagat ctccagccac
    63721 ccaggtctgg ccgatcagct cgtaatgcat tccccaccaa caggccgctg aaagactacc
    63781 tatgtttctc aacagtcggc taaccacttc aggtgcgaaa atgcccaact gcatcattca
    63841 actcaccgcc aacccgttgc aactcggccg tcgagctttg caattgctgg ctttcccgcg
    63901 cgctgtcctc agccacttcg cgcacccgcg tcatgctctg actgacctgc tcagctacca
    63961 cgctctgttg ctcggcggcc gcagcaatct gttggttcat ctgttcgatg ctggataccg
    64021 cgtgggtgat ccgcgccagc gcctgggatg cctgagcggc caggataacg gtttcatcgg
    64081 tcagcgcatg gctgccctgc agacgttcgg cggcttgctg ggcgacgccg cgcagacggc
    64141 caatcaaccc ctcgatttct ttggtggaac tctgcgtgcg ccgcgccaag ccgcgcactt
    64201 catcggcgac cacggcaaaa ccacggccat gctcgccagc gcgcgcagcc tcaatagcgg
    64261 cattcagtgc cagcaggttg gtctgttcgg ccaccgcctt gatcacatca agcacgccac
    64321 caatcgcggc gctttcgctc agcagcgatt gcatagcttc ggcgcaaccg gtcatttcca
    64381 cggccaagcg gtcgatcttg ccgccggcct gacgcaccag ctcatcgccc tcgcgcgcct
    64441 ggtcatcggc ctgagccact gcggcactgg cctcgccggc gttgcgcgcg acgtcctgag
    64501 cagtggcgct catctgctgc atggcagtgg cggccatgtc ggtctcctga cgctgctgct
    64561 cgacaccacg gctgttgcgc tcaatcattt caaccaggct ggcggccgtg ccattgagct
    64621 ggccgacgcc accgccaatg cgcccaacca gcccgcgcaa actgccgagc atgcgggcca
    64681 cggtgtcgag caactggccg agctcatcac gtcgcgtatc cccttcctgc gcctggctca
    64741 gatcccccgc cgccacccgc tgggccaatt gcacggcctg gcgcagcggc tgcaggatca
    64801 gttgacggat cagcaggctg gcaccgatgc ccagggccag cgccgacaat gtgatcaacc
    64861 cgagctgacg ctgggcatcc tgattgtctt gggcaatcgc ctgtgtttgc tgctggtcgg
    64921 cctcggccaa cagttcgctg acccgctcgg tttgcatgtt catcgccgcg ttgctgcggg
    64981 cgctctgccc acggctggcg acaaattgtt cgaaagcctt acggtaattg cccaaagcat
    65041 cgcgcgcgcc ctgcagagac gtctgatcct gatcgccgag atccagcgcc tgcatggccg
    65101 acagcaggtc gctcatgctc atttcccagt cgctgcggta gcgctcctcg ccgtccagtg
    65161 agtagtacaa ctcgctgtcg cgcagcttgg ccagcttgtc gcgcagagca gcggtctgct
    65221 ccagcagcac catctgctcg ctgctcggtg atatgtcctg ctccagttgc gcattgagcg
    65281 catccagctg atcgagaaac agcacggtaa aactgtcgcc cgccgtctgc gccaatgtct
    65341 gcatgcgcag gcgcgcctca cgagcctggc gtagcgagtc ggcataaccg agaaactgct
    65401 gcaggtaggc gctggcggca tcgcccatgg ccacgtgggg agcgctgctt gaagaagcgt
    65461 cgagtagccg gatcagttgt tcgaccacct cgcgcacctg gatcaatgcg ctcggtgcca
    65521 ggttcagggc gaactccttc tcggcgatcc ttgcctgcag aatcaacacc tgggtcgctg
    65581 cctcgtccct tgactgctcg ccccgcgcct gcagcacccc gagagaatga aatgcggtaa
    65641 gcgccacacc cagggtgaac agcaacacca ggccaaagcc cagcgacaac ttggcaccca
    65701 cggacagatt accgagcacg cgactcagtg cagacattcc aacctcctgg ctcgatacat
    65761 agcgaacacc gcctctcgac agtgacaaca tcgttcaatc tcagcccggt tcagttgccg
    65821 tgggcagggg ctgcctggga aaaccccgtt atcgggccgc caaccctagc ggcccctgtg
    65881 ccgcccgtac caatcgcatt gctcattacg aggtatcgcc cggcgcgatg ccaaccagcg
    65941 accacgttcc tgacattcga agcgaagccg gtactggcta tacctgtcat ataacgtgca
    66001 gaacttgagg gttgtgaagt gagctggcaa atggcaccac ggcgtacgct gcgcagcgga
    66061 acaaagcctc agcgcaagat agagtcgata ccaatgagcg cggaagatga cgccgtgggt
    66121 aaacaacaaa ccaatgccgc atagctgaac cgtgcctaga ctaaataacg gaacccaagg
    66181 tgatgccgtc tctagcgaca agccggtaac tcaacttgag caagccatca ccacagggag
    66241 tgcctttcat gctgcagcta ttcactgacc tcaagttacg ctggagagtc gccacaacca
    66301 gtgaagagcg cagcccttgg gccgctgcga ggccaacacc aagtgataaa ccagccatat
    66361 cgcgcatcgc gctctcattc atagacaagg ccttgcgcgc gaaaacacgc gccttcgcgg
    66421 ggttcttcaa gcggttagcc gccctattga tggtcatcgc ctcttgcact catgcggccg
    66481 aatcgccgac agccctgagg tttgcgaccg tcgataactt cccacccttt tcctacatgg
    66541 agaacggcaa cctgaccggt atcgatatcg atctggtgta tgagatggcc agacgcatca
    66601 acatctcagt gcagatacag acgtacccct gggcgagagt catgggctcc gtcaaaagcg
    66661 gagagttcga cggggccttc gctgccttcg agacggcaga gcgacaagcg ttctgcctgt
    66721 atgtgggggt actgcatgtc gaggagttct acctcttcgt ccgtaaagac aatgcatttc
    66781 cctatacgca gattcccgac ctgtatggca agcgggttgg gatagaccgc ggggtctatg
    66841 tcggtgaggc cttcgaacgg gcggttaacg acgggaggat cgcactggag gaagtcaatg
    66901 acatgggcat gatcaatatc cgcaagctca acgccgggcg tatagatgcg gttatcggcg
    66961 accttggggt gatgaactac catctgaagc tgttgggcct ggagcaacgt atcgttgcgc
    67021 tccagccgat acgggaaccc actccagctt atctggtact gtccaaggct tcgcagctga
    67081 aaaacaaggt cgagttgcaa gaccgcatgc gcagagctct acatgacatg tggaacgacg
    67141 gcacctacca cctcatctat aaccggtatg tctacaaagt cggtggcgac ccgcttgccc
    67201 gataattccc tacgaagtgg cgcgggtgaa agaaccccat tgcatgcccg ctcgatccac
    67261 gttggatatt gagaaggctg atatttagca actcgcatca caagcacctc ctgccgcccc
    67321 gctacatatg acctgccagg ttatccaacg acatccattg ccaggccgta gtgaaacggc
    67381 ccggcataaa agccgggcct gagatcaatc acataccggt gcggatggca ttccaggtgc
    67441 gcatgcgcat gcgttcgaca ttttgcggca gcgccttgag cacaaacagc gaaggcatga
    67501 cgtctgccgg cacatacata ttcggattgt cgcgaatgtg ggcatcgacc agttctgtag
    67561 cgtccttatt ggcattggga taaccaatgg cgttactggc cttggcgatc acatccggcc
    67621 gcatcatgta gttcagaaac tcatgggcct cgtcacgatt gaccgcgccc ttggcaatcg
    67681 ccatcacgtc cgaccaaaca ggtgcgcctt ccttcggcag aatcatgtca accttgacgc
    67741 ctttacccgc cgcatccgcc aagcgtgttg ccagggcaac accgcctgac cagccaatac
    67801 ctacgcagat atcaccattg gccaggtcca tgccatagcg cgatgagttg aagtaattga
    67861 tgtgcggacg aatcgaggcc atcaattcct gagcctggcg ataatcgctt gcctcagtgg
    67921 agtgcgacgg taagcccaga taatgcaggg ctattggcag tacctcgttg gccgcatcga
    67981 taaagccaac accgcacttg gacagcttgg cgatattatc ttccttgaag atcagatccc
    68041 aactatccag cttggcatcc gggccaagcg ccttacgcac catatcgacg ttatagccaa
    68101 tcagcgtggt cccccacata tacggaatgg cgtactgatt acccgggtcg ccactgctgg
    68161 ccaagtcagc cataaacggc tcatccaggt gagaccagtt tttcaacttg ctgcggtcga
    68221 gcggctcgaa tgtgccggcc tggataaatt tgcccaggtt ggcggtactg ggaaacacca
    68281 cgtcatagcc cgaattacct gtcagtaact tggactcaag catttcgata ctgtcaaaca
    68341 catcataaat ggggcgaatg ccggtctccg cttcgaagtc cttgaggatc tctccgggta
    68401 ggtactccaa ccagttgtag attcgaacgg ttcgttgctc ggcaaacacc gagccactca
    68461 tcagggtgac ggcaatcgcg gccgccatgc tgcgcattac agaattcata tcagactccc
    68521 ggggcaccca ccacagcggg tacgcactta tcaatagcgg taggtgaagt acatttcaac
    68581 ggcggagaat gtcttgtcgc ccaacagttg cttggcggct gctttcggct gcacccagtt
    68641 gtaggacagc gaggtgaaca gctgaggggt tgccacccag tcgacaaaga tcacgctttc
    68701 gtcggcgaaa cgccggtcac tgacaggagc accgaagaga ttcttttcat cgagagagaa
    68761 ctggtgatgc atcgccccga cggtgagcgt tggttgtacc tgagttttca acgaccaggt
    68821 atgcaccttc tggttactgt tgttaagcag gtagttgccc accacatcac cgaccagcca
    68881 ggtgccccag tcgacaaagc ctttgctcag agcgtcccag gattcacgct tgttgtcggc
    68941 caggtcttcg tcgccggaga aataggcgta gcgatagccc agtctcggct gtaggggcag
    69001 atcactgaaa gcgtactcag cccctgcata ccaggctttg gcgtcgtact cgataccgcc
    69061 gtccctgccc ccttgcacgg catactcggc gttaagggtc aggtcctcaa ccccgggcag
    69121 cttggcattc agtgctcgaa cgttgtagac ctccatgcca tcgcgtggtg ttgcgatatt
    69181 ggagtcacgg tcttccgctt tcagcttcat ggccatcgcg ccgaaggtgg caacaccttc
    69241 caccgcgtaa tcgaggttca cgccattcat gcggtagtcg ccaagatggt catcggtgcg
    69301 caggctgaag gcctgcaccg agagcgcatc acgcgaccag cccagcacag cggagtcacg
    69361 gaaggccgtg cgcggtccca accagaaggc cgcgtcatcg tgaaagtcga gattgccatc
    69421 catgacaata aagcccgaac cgatcatgta gtcctggcgg cctgcggtaa aacgccattc
    69481 cccggcgcga aagcccacgt aggcttcctc tgccgagaca cgattgtcgg taccgcgggt
    69541 gaaaccgcct gcgtcgccat cgccgaaggt cgatgcagcg acgatcgagc ctttggccaa
    69601 cagatcgaag tccgggttca gtgcatagct gaaactgacc gccggcttga catagacctc
    69661 ctgccagtcg gcattgtttc cggtggcctc accgttacgg gtatcgacac gccccgcacc
    69721 gaagttgacg ttacgggtga aaattgcagc accgcccgcg gtcaggttga cctcaccaga
    69781 aagatcgccc tcctggaagg tatagccagc cacagcagac tgactagcca agaccgctgt
    69841 cgcaagaagt gaaggtagca agggtactcg tgtcatgggt tgctcctggt tgattgtttt
    69901 tgtgtcacag ggcagtttca aagcgacaac cgtcgccatc gcgaatgctg gcgggccaag
    69961 ggtccgccag cagatcttct tgtagtgtcg aatcagcccc ggaagtggtg ggctagtgca
    70021 cgcagtgcgc gaacgttgtc ggggtcgaag aggctgacgg cctccggcag cgaagactgc
    70081 ttgacgatca ccagctcggc ctcgcgatcg acgaacaggt actgaccgtt gatgccgcat
    70141 gccatcaggg ccgggctgtg atcgttgaac acgtaccact ggctgcggta cgaagctccc
    70201 ggcgtccagg cgctgaactc ctcgttgccg gcatagatcg ccggatcgct gccctgggcg
    70261 atggcatcga tcgcacgtgg atcgagcact gcgcgtccct catgcacacc gcggttggcc
    70321 agtagcaggc caaagcgcgc gatatcccgt gcggtagcgt tgaagcctgc accggcaaca
    70381 ccgcgccccc aggggtcggc cataaagtaa ccgtcacgct gacagccgat ttgcccccag
    70441 acgcgcgcca gcaactcggg acaggcaatg cccccagcac gctccatcac ccaggccaga
    70501 gcttcggtag ttgcagtcac atagtggaaa aagccgccgt gctcgccact cttacgcagc
    70561 gacggcaagt actgatagag cgaagcgaac tgggcgtagg ccgcaggcgc cggctggaaa
    70621 ccacaggcat agccgaattg cgagctttcc gaagccggat cgtcgtaacg ctcggtgtag
    70681 gcgatcccca ccgccatatc caggagctgg cggacggtcg catcggcgaa agcgctgccg
    70741 gcgaattcgg gcacatagtg gtcggccctc agctccggat tcaacacgcc gtcgcagatc
    70801 agttgttcgg ctagcacacc gatcagcgac ttggtcaccg agaacatggt gtggcggtca
    70861 ttcgcagtct gcccgttgaa gtaacgctcg tatttcacct tgccgcgatg cagcacgata
    70921 aaggcatcgc agtggctggc cacaaggtgt tcatcgaggc gaacggttag cccacatacg
    70981 ctctcgaagc tgaaaccatc gaggtcttcg gcggcctcag gtaagtcgct cgctgcgccg
    71041 ttaccggcgg cgacagagat gcttgggcgc agccgtgcaa gatggcggaa gccccatttg
    71101 ttgaacggtg gactcatcca gttgtgccag gtaacgcgct gcgcctctgc aggcgggaag
    71161 cccgtcataa aggcatcggg ctgggccccg gcgtgcagcg cctcatgggc atcagcataa
    71221 tggcgagcca gttggcacgc ggtgggggaa tcggtgcgaa gtgtcataaa tcagctgtcc
    71281 tgacagatgc tctgcacgcg gcagatgtga cagaaaaatt aatccacata atttttttag
    71341 tcaataaaat atttttgaag aaaagagctt tccgctatgc tcccgctgcc aaggcaggag
    71401 aacccagggg gaataattga gcggcttgcg agaacgacag aaagtgatgc gccgacaagc
    71461 catcagccga gcaggggtaa aactgttcga gcggcagggt ttccagagca ccaccatcga
    71521 gcagattgcc caggaggccg gcgtttcggc accgacggtg ttcaagtact tcggctccaa
    71581 gcacgagatc ctgctcgaga tgctgcaggc cggcgacgag cgcgccatca gcgatgcacg
    71641 tgcacatatt gccgagatgg atgacccgct ggatgccttg tgctatctgg aaaaggtgct
    71701 ggtggatcag gccctggcca tgatgccgcc gcctatctgg cgtgagctgc tgctgcatgt
    71761 gctgggcggc ggacaaagtg gcctgccgga cagctacaaa cgcatgaatg acatcctgcg
    71821 cagtgaaatt gccgcactcc tgcgcgacct gcagagcgca ggcaagctgc gtgctgatct
    71881 agatgtggat ctggccgcct ttctgctcaa cgactactca cacctgcagc tactgcgcct
    71941 gaccagcaac gagccgctcg accttgaaac gcatcgtcag caggtacgcc agacgtccgg
    72001 cctgattctc tatggcatgg ctgcagccaa gccctgacaa atcgccagcc tcacaatcac
    72061 gggctcggac tcgacatcca gggctatgtg cgaaagcgcg acaacagccg cacctgagcc
    72121 gcagcctgct cgtcgatggc ttgtgcctgc tgggctgaaa caccggctga gcgggtaagc
    72181 tcatcgctca ccgcacgtat gcgatcgact gaggtactta tttcctcggc caccgcgctt
    72241 tgctcctccg ccgcactggc gatctggccg ttcatctggg caatctccat caccgcgcta
    72301 ccgacgccgt acaacgactc caccagttcg gtaaatagtt ggtcagcctc actgaccagt
    72361 tgctgattac ttgccatcga acgaaccact gactgcatgt cgccctgcag cgcctcgatg
    72421 atgccgtgga tctcctgaat cgagtcctgg gtacgctgcg ccaggccacg cacttcatcg
    72481 gcgacaaccg caaaaccgcg cccttgatcc ccggcacggg ccgcttcgat ggcggcattc
    72541 aaggccagca aattggtctg ctcggcaatt ccacgtatca ggctgagcac gacgctgatc
    72601 cgctcgctct tgagcgagag cgcttttacc tgatcattgc tggccagcaa gtcaacgcct
    72661 tgccgggcaa caatcgccgt cgttcgatca atcaggtccg tgccatcttc aatggtgcgc
    72721 aaaatgccat cggtggctgc agcagcagag gccgtgttac gcgccacctc atgggcagat
    72781 aggcgcatct gctgcatcgc cgtggcgacg tgatctatat cgctgcgctg ccgctctgtt
    72841 gccgccaggt tggctttccc tcccgctacg gagtgaacag ctgcggcact gagcgcttgc
    72901 atggaggttt tgatgctggc aatagtcggc tgcaagacct caagaaaccg attcatcccg
    72961 cagcccaagg cacccaactc atcctctcgg ggatagttga tacgccgcga cagatcacct
    73021 tcaccatccg ccaactcgtc gagcacggcg gccataccct gcaatgggtt gacgattgcc
    73081 cgcgacgcaa aagcaattag ggcaaggcca agcagactca ccgccacgcc caggatcaac
    73141 tcgaagcgaa ttgaccgttg cagttgctca ctgagggttt gctgcaggct gatcactggt
    73201 tcgaatgcct gctccgccgg cacctgtacc cgcacgtgcc aaggctgcgc agaaggaatc
    73261 ggctgaaacg actgctcggc atggatgtcc tgcaggccat ctgctcgaac ggccttggct
    73321 ggtgcttgcc acggctgccc gagaaggcct gtatcagcgg tggtggccgc aaccaaacca
    73381 gcatcactga gcagccagac caagccaccc tgcccgggca atgcttgctg cgcacgctgg
    73441 gccagaacct gcagctgatc gagctggata tcgacgccca acaccgcgac caccacaccg
    73501 tcgatgcgca ccggcagcgt caggctggtc atcagcacag gctgacctga agccgtatcc
    73561 agataagggc tgagcaaaca cggggctgcg ctctgcagag ggcaatcgaa ccaggtgcgt
    73621 gctggccgcc catcgctcat gggcgtctca tcggccagcg tcttctccgc cgccgcgacc
    73681 tgttccagcg tctgtggccc acgacgaaac cacaccgggg caaagcgccc gcgctcatta
    73741 ctacccttag cggtcagatc gacaaactcg gcatcgcgac catccagcgc gccgggcaga
    73801 aacacaagat agctgctgag caggcctggg ttgtcatcta gaagcgagcg gatctgagcg
    73861 gtcagatgct cgcgcatggg tacatgctca tggggcagcc cacgcaccag taacacaacc
    73921 cgctcgacct ccgtggcggc ctgctcacct gcgcgcagcg tatcggcaaa aaatgcctgt
    73981 acgctggcgg cctgggaccg cacagactcg cccacacggg cgtctatcga cgcgccaaga
    74041 ctgatcgaag cctgctgctc cgcctggcga ctcagtgttc gcatttcgta aacggaggcc
    74101 gcgatgaccg tcaacgccag ccccgccaga catgacccaa ccagaagaat gactttcgcc
    74161 cgcagtgtca tcggcaatgc ctccgctcat aaataaaaaa ggcaacgtcc ctgttaccaa
    74221 cccaaaggat ctggcttgta atggtctcga atacggatta cctggctgga acccggacaa
    74281 cccgcgacag ggcactcaga ccgcttctgt ccgcagtgca ataggcccca ggctgtaaca
    74341 ctgaatcagt acttcactcg cgctggggta gtgcaccaac ccgataaagg catgctgtgt
    74401 cagccagcca agtggccctt ctgccacttg aaaacgggga gtacaggtgc agttgtactc
    74461 ggacgcgggg gtgggccaaa tgccctgagc gtccagctca cgccctcgct cagtcatcgt
    74521 cagctggcca atattgtgga tgttgatcaa caccccatcc tgagtgcgca gctggtagcg
    74581 agcatccagg tcacctacac cgtcctggcg cagcaaaaag cagtctgcgc cgccgggcag
    74641 aacctcccca cgcaagtgag gaccgctgaa gtgcccccct atgatcggat aattgatccg
    74701 gtatccatcc acgcaccggc ccaggacttc aggctctgcg atcaacgcag tgatactcat
    74761 caccggatgc agcaaaggaa tcgcaatatc gctcacggtg acactccaac tcgccagcgg
    74821 tgcatgcgga ggtcgctgcc tcgctcaagg gtggtttcgt ggtctcgcgc cagatgcgtg
    74881 cacgcacacg ctctatcggc gcgccgaccg gctccagggt aaatagcgtc tggcgcacgg
    74941 ccggtggcat aaacatcacg gggttagggc gaggcgcagt cttaagtaac tcggtcgcat
    75001 ccttgttggg gttgggatag ccgatgctat tgctgatgcg tgcaatcaca tctggacgca
    75061 ggatgtaatt gataaaggca tgcgcttcct cccgattggg tgccttgcgg gccacgacca
    75121 tgacgtccga ccatatcggt gccccttcac gtggcagcac catctcgata cgcacgccct
    75181 gcccggccgc atcggctaac tgctgagcga ggacaaagcc gcccgaccag ccaagcgcga
    75241 gacagatctc gccactggcc agatcatggc catagcgtgt ggagtcgaaa tagcgaaccg
    75301 agggacgaat gcgggccagt gcctgctgcg cctgtttgta gtcctggcgc tggcgactat
    75361 tgggatcgag gcccaggtgc tgcaacagga tcggcagaac ctcgctgggc gcatcgagca
    75421 tggccacgcc gcaatccgcc agtttggcga ggcgctgcgc atcgaagatc aacgaccagc
    75481 tagccacatc cgcatccggt ccgagggcct ggcgaagctt gtcgacgttg taaccaatca
    75541 acgtggtgcc ccacagataa ggaacggcgt agcggttacc cggatcaccc gccacctcca
    75601 ggtggctcat aaagggctca tccaggtgac gccaattggc tagccactca cgtttgagcg
    75661 gctccaacgc ccccgctaca accaacttat cgaggctgta ggtaccggga aaaacaacgt
    75721 catagcccga gttaccggtt agcagcttgg cttccatcgc ctcgacgctg tcgaactgcg
    75781 tgtaaaccgg atggataccc gtctcgcgct caaaactgtc gagtacctcc tgcggcaggt
    75841 actcgatcca gttgtagata cgcaccgtac ggggttgccc agcggcaccc gtggaggcaa
    75901 gcgatgtgag cagtagcacg gcggccaaca gcatgctcca ggcggggcga tatgccacgg
    75961 agataggggg cgaaacccac ccggcgttca acctcatcac ggttctccag cagcttattg
    76021 ttatgtgctt gcctgcgtgc ccttggaata ctgcgcgaaa cattcattgt ccgcgcagca
    76081 ctatttaatc gattaaaata ttttattcaa taaaatgttt tagccaaatg cttggcatct
    76141 tgacgcccgg gagttgacca acacttctag tggctcattc aggcccgaaa ctgtgcaaca
    76201 gctgccaaca gttcccgctc cagccccgcc agttcgtcac tggccacgac catttgttgg
    76261 ttagcatcag caaagcgttc cgcttcatcg cgcaggtgca tgacgttgcg gctgacctct
    76321 tcggcgacga tgctctgctg ctcggcgctc gccgcgatct gctggttcat ctgctcgatc
    76381 accgacaccg ccccggtaat gccctgcagg gccgaaccca cctgatcgga caggccgacg
    76441 gtggactgca tgctgcgcgc acacgcctgg atctgcgcaa cgctgtgcag cgaaaggttc
    76501 tgcaggcccg ctatcagggt ttcgatctca cctgtcgata cctgggtgcg cttggccagg
    76561 gagcgtacct cgtcggcgac gacggcaaaa ccgcgaccct gctcgcccgc tctggcggct
    76621 tcaatggcgg cattgagcgc cagtagattg gtctgctcgg ccaccgcttt gatcacgtcg
    76681 accaccgtac cgatgcgcag cacctgctgc tgcagagact gcaccgcctg ctcggaaagc
    76741 tgcatatcct cggccaggcg ataaatatgc tcgcgggtct gctgcacgat ctggtcgctg
    76801 ttgcgcgctt gcctatcagc ctccagcgca gactgcgaag cctgctcggt attttgcgcc
    76861 acctgctgca ccgttgcggc catctgctgc atggccgtgg ccacctgctc ggtctcgacc
    76921 cgctggcgct gcgcgccatt attgctttgc tcgctaagcc cgcccagggt cgcggtcgac
    76981 tgcccaaggc cggtgacgct ttcacctata cggctgacca accgccgcag gttggcagtc
    77041 atctcggcca tcgccgccat cagttgcccc aactcatcgc ggcgcactac cccactctgc
    77101 atatccaggc tcaggtcgcc ggcggcaata cgccgagcga cctgcagggt ttcccgcagc
    77161 ggggccacga tcagattgcg gatggtcaat gaggccagca gcgccagggt gacgatcacc
    77221 agcgccccca ccgcctgcag cagcatcact gagcggccgc gctcgtccat ctgcacctgg
    77281 cgttcggcag cggcctgttc gacctgctgc aatacgcttt gcgcctgagc atccatctgc
    77341 agcgccgtct gctggctcaa tgtcaggcta tcgcgatagc gctgaaacgc ctggcgatat
    77401 tccaccagcg ccgccagggc agcggccagg gattcttcgg agttttcgtc gacccggccc
    77461 tggagcagct ccatggcatt gctcatttcc tcggttgact cgcgccaacg ttcgagggcg
    77521 gctggctggc ccgtctgcac atagaggaac tcgctattgc gggtaaccag caacttgcgc
    77581 tgcagtgccg aggcctgttc ggccaggctc aacggatccc cctccagcgc ctggccactg
    77641 ttgaggacgc cacgcacctg gtcgtacata tccagctcga ccatctcgaa ctgcaggcgc
    77701 gcctgctcgg cctgctcttg catatgttgc aatgcctcgg tcgccaggcg ctgatggccg
    77761 acaaactgct cgaacaactg ctggtaaccg acgatggctt gctgcatggc caccagcacc
    77821 ggcaaagcat cggccgtagc ttgcgcttgt agttcgcgca actggctgtc gagcgtatgc
    77881 accagcgcct cgacctcctg catggccgtt acagtgggtt caagggcata gcgcttctcg
    77941 gcattgcgaa tctgcaaggt caggcgattg accgccgcca tggcgctgac ctgccggccg
    78001 tgttcgagca ggctggccgt ggcatacaag ccctgggcaa tggcagcgac cgcgagtacg
    78061 gacaccaaac caaaaccgag caacaacttc tgccccaccg aacagttggc gagcagcctg
    78121 gcgattggct tcagcattgt ttttatcctt atgtctgaag tgttgcgttc tgcggcgcta
    78181 ctggactcct ggtgcgcacg gcgcacccta ccccaaggtc gaaggtcgct aacgcgccgc
    78241 cagcttatcg agcgccacga ctatgccggc caggtaacgc tccaggctgc cggtcaccct
    78301 gccaggatcg cgatcaagca aacgctggcg cacgaagcgg taggccaagc gctgtttgtg
    78361 cagcgtatcg gcctgctccg gcagcagtgc tatcagctgc acaaacccct cctcaatggc
    78421 ttgatcccgc gcggtgaact cgacctcgga gtaggccaac gagtgatcgc cgagtacccg
    78481 cgcactgcgc gcctgggcat gcagcaacag gctggcgata tccaggctct gccgattgag
    78541 cagacgcacc acgggctgca gatcggcctg cacttgctga taggccgcgt cgctctgccg
    78601 gtccagctgc gctcggctat cgagcaaacc gatcaacagc tgcgggtaac gcggcgcctc
    78661 gtcacggcgt acggcctgca aatcgtccag ccaggcattc aggctggcca gcgagtcgcc
    78721 catgtccgcc ggtaagttga gttgcagcgc cagtgcatcc aggcgctgct gcgcttcgcg
    78781 cacgctggcc agatggcgtg catcgggctg cgcgcgcggg tcggcatcga agtagagcaa
    78841 cgcattcacc gccagcaggg tcgcctggct gcgcagctgc tggtactgct gcgcaggcgc
    78901 tgcggcccag agcgtcgaag acgccaacaa ccaaaggctc atacaccctc cctgcagggc
    78961 gcgcattcag ctcaccccgt caggctgcac ggcaggcacg tcgcccgact tggcaacgaa
    79021 gagtttctgc ggcatcagaa aataggccaa agccggcagc aggatcagcg cgccgagcat
    79081 gttcatcagg aacataaagg ccagcaggat gcccatatcg gcctggaact tgatcggcga
    79141 gaacacccag gtggccaccg caatcgccag ggtgatgccg gtcagtacca ccaccttgcc
    79201 ggtgaacagc agcgcccggt agtaagcctc ggacagcgag tcgccggcac gcatgcgcgc
    79261 caggatcacg ctcagcacat agagcgcgta atcgatgccg atgcccacac cgagggcgat
    79321 taccggcagg gtggcgacct tgacgcccat gcccaggccg accatcagcg cctcggcgag
    79381 gatcgaggtg agcatcagtg gcaggatcgc caccagcacc gcgcgccagc tgcggaaggt
    79441 cacccaacac agcaggatca ccacgccgta gacccaatag agcatttcct gcatggcctg
    79501 ctcgaccacg atattggtcg ccgcctcgat cccggcgctg cctgcggcga gcataaactt
    79561 cacctccggc agctgctgct cggcgataaa ggcttcgctg gtggccacta cccggctcag
    79621 ggtttcggcc ttgtggtcgg caaggtacac atacagcgac agcagcgagc agccctggtt
    79681 gaacagctcg cgcggcgcgc gggtctgtac cgcgccgaga gcaccgtcgt tgggggtcag
    79741 ctcgtaccac ttgaagttgc cttcgttgta gccggcggtg gcgatcttgc tcagcgccgc
    79801 catggagttg gtagactcca cgccgggcag ctgctccagc cgccaggcca gttggtctac
    79861 cgccgacagg gtgctgtagc gcgtgcattg atcctcgggg gtgcggatca tcaccacaaa
    79921 gatgtcggag ctggcggcgt agttgtcgtt cataaactgc acgtcgcggt tgtaacgcga
    79981 gtccgcgcgc agctccggtg cgccgggatc gagatcgccg atctgcagct ggctggatac
    80041 ctggttacca aacaccgcca acagcaggcc caccagcacg gcagcgatgg cccagctgcg
    80101 gctggtaaag cggtcgagca ggcgccatag cggatggcgg gccacgccgg cgcggtcgtc
    80161 ttgctcggca tgtaggctgc gctgcgcagc cttggggctg acgccggtgt aactcaagag
    80221 caccggcagc agtaccaggt tggtcaggat caatacggcc acgccgatac tggcggtgat
    80281 cgccaggtcc tggatcacct ggatctcgat caccatcagc acggcgaaac ccaccgcatc
    80341 gctgatcagc gcggtgaggc cggcgagaaa caggcggcga aaagtcagcc gggcggcgat
    80401 ccaaggcagc atgccgcggc cgatgtcctg catgatgccg ttcatcttct gcgagccgtg
    80461 gctcatgccg atggcgaaca ccagaaacgg caccagaatc gagtacggat ccagctcgta
    80521 gcccatggcc gcgagcagcc cgacctgcca gagcaccccg accagcgaac agagcaccac
    80581 gacgaaggtg ctgcgcacgc agcgggtgta ccagaacagc acggcgacgg ttatcgccac
    80641 cgcggcaacg aagaacaacg ccacctgcag cagcccttcg atcaagtcac cgatcagctt
    80701 ggtaaagccg gtgatatgga tgcgcacctg ctcgttctgg tacttgtcgc gcaaggcctc
    80761 cagacgccgc gagaattcgc cggcatccag ggttttgccg gtttccgggt tgatctccag
    80821 cagcggcacg tagatcacgc tggacttgaa gttggcggct accagttggc caatctcgcc
    80881 ggagcgggcg acgttggtgc gcacctcgtc caggctttcg ggcgtgccgt cgtagctgcc
    80941 gggaatcacc gtgccgccgt ccagaccgtc ctcggtcacc gcggtccagc gggtggtcgg
    81001 cgtccacaac gacttcatat aaggcttgtc gacgcccggc agcaggtaga tctcatcgtt
    81061 gagtttggcc agggtgtcga gatattcggc gctgaagata tcgccctgct ccgccgtcac
    81121 ggcgatgcgc aaggcattgc ccaggccgct cagctcgctg cgctcggcga ggaaattgac
    81181 cacgaaggga tgcttggtgg ggatggtctt ttcgtagcta gcgttgatgc cgatgctcgc
    81241 ggcttgccag ccgagcacca gggtggccaa cagacatagc aggatcaccc agggccggtg
    81301 gttgaagatc agccgctcta cccggttgcc cgaggcgcgg tcgaagtctt gcaggctgcc
    81361 gctcaggctg tgcatggcgc tcgacggttt ttcgtatttc acatctgact cctaacgctc
    81421 aagggcgggc cgaagcctgg atttcctggc gggcggacaa gccgcccaga ccgacgctgg
    81481 tcaggctgcc atcggccgcc tgtgcaacgg cggcaaccgt agcgccctgg tgggtgcggc
    81541 ggtgctcgaa gtgcaggccg ccatcgccgc tgagcagcaa ttcgccgctc tggctggcca
    81601 gcagcaagcg accgtcgctt agctcaaggc ctgcggccag ggccgactcc accccggtgt
    81661 ccaactgctg ccagctgtcg ccgcggtcac tcgaccacca ggcgttgccg cgcaggccat
    81721 aggccagcag gccgccgctg cggctggcca gcaaaccgaa aaagctgccc tcatagggcg
    81781 attccagctt ctgaaacgac tgcccagcat cgctggaacg cagcagcaaa ccgcgctcgc
    81841 cggcgataaa cagctcgcca ccctgggcgc gaatgccgta caggttgagg ccttcggggt
    81901 tttccacctg gcccatccag gcttgccaac tggcgccgcc atccgtggtg cgcaggatca
    81961 ggccgtaggc accgacgatg tagccatgct gacggtcgct gaaatacagg tcgagaaagg
    82021 gtttgtccgg gccatcggcg accagccatt cggcggcctt gagcagcctg gcgtcgtcgc
    82081 cctgctgggc cgaggccagt gccaaccgcg cggcctgaat gccgtccagt tgtttgttcc
    82141 aggtctcgcc gccgtccgcg ctgtgcagca ccacgccgag gtggcccacc gcccagccct
    82201 gttcggcatc gacaaattgc accgaggtca ggctgacgct ggccggtacc tgggcttggc
    82261 gccaattagc cccggaatcg tccgagagca gaatgatgcc gcgctcgcca accgccacca
    82321 gacgttcgcc ggcgcgagtc aaggccagca gtaccgagcg cggggccttg gcactctgta
    82381 gcgccggctg gtcgagtaaa ggaatcttcg cgacgcccgc cgactgcgcc atcgcccaac
    82441 cgggcagcag cagcgcgctc aatagcaggg gcaaagcccg tgtaaaggaa aaagtaccga
    82501 tcatatgcgc tccaaccact gcagaagaat tagccaaaca acacgccaga gccttggccc
    82561 cggcgtgcct gtgagtggct tagcgcatgc tgtcgctggc catggcatca ggggtgaaga
    82621 acgacgccga cggacgcggg tagtcctggt actgcacctt gaggtcgttg gccgaggcat
    82681 tgaggaagta ggcaccggtc tccaggttgt agctgcccca catgacgttg gcggtcagca
    82741 ccggcatttc cggcgcgagc agggtcagcg agtagttatg ccggcccaac ttgccctgcg
    82801 cgtcataacc atcgatcagc agtacctgcc aggagtcctc gtccacgtag taggtgcgct
    82861 tgggcaccac atggcgcttg ccggccttaa gggtggcctc gactacccac acgcggtgct
    82921 tctcccagcg caccagatcg gggttgagaa agcccggctt gaccagctcg tcgctgctcg
    82981 ccgccgcggc acggttgttg ttgtagggca ccagcagttc cttcttgccc accagcttga
    83041 ggtcgtggcg gtcggtcggg ccgaagatca taaaggcctc gtcgaagaag ccgacgccgg
    83101 aggtgacgaa gtccggggtg tcgtaggcaa cgttcggcgc gcggcgcacc cggcgttggc
    83161 cgaccagata ttgccaggcg gcgcgtgcac tcgggtccat gtcccagtgg gtcatcaggc
    83221 cttcaccggc cttggacgat ggttgctcgg tgagcagctt gccgatcagg tgcttgccgc
    83281 cataggtctc cagactgcct tccttgaagt agtaagggtg ctggtaccag aagcgcgcgc
    83341 gggtggcttg cagcttcttg ccaccggcgg tcatcagcca ggtgtcgaag ggcacgtaga
    83401 aagtatcggc accctgccag gacaggcggt agttccacag cacctcggca ccgttctgcg
    83461 gtatcggaaa gggaatgccg ccgtaagcgc cgctaacttt ttcggtggcc tcttcgaggg
    83521 tggcgcgggt ggcgttctgc agggtgttgt cgtacaccca ctgcggcgcg gcggcgctgc
    83581 gatgggtcgg gtagacgtcc aggcggtagt ccgggtactt cttcaacagc gcctgagtgc
    83641 cttcagccaa ctgctcggcg tactcggcca tattgctcgc gctgaccgag aacaaaggct
    83701 tgtcagcggc aaaggggtcg gcgcgtttgt cgccgctctt gtagccggcc ggcacctggg
    83761 tgtagccacc ggtccattca gggatgctgc cgtcggcatt ggcggcgcgt tcagccccca
    83821 taggtgtgag gtcggcattg aggcgcgcgg cctgatccgg ggagaccgcc gcatgggcaa
    83881 aacagctgcc catcagggct atcgtcagga cagagagacg caaggaaagt tggctttgca
    83941 tggcgaataa aacctcttat tagaatgtgg ttttcacgta gacggagacg aaatcgcggt
    84001 cggccagcga ctgcgcatag ctgaaattgc cgtcctcgcg cagcccagcg cccttcttgc
    84061 cgaagaagtt cacgtagttg acgccgaagt cccagcgctg caggtaggtg gccttgagcc
    84121 cgacgctcca gtcgccggcg tgactgttgc caaaaccgga cttgcttacc gccgaggagc
    84181 gcccatcgag caccacaccc aggcccaccg gcacgctcag gtcgaggccg tcgaccacct
    84241 gaaagtacgc cggctcggcc aaaatgcgca gcgccgtggc gtcgcgggtg gtgttggaat
    84301 ccagcgccgc acggttgtcg gttacgctca aggtgcggtt ccaggccaac tcggcgagta
    84361 ccgaaccgcc atcccagagt gcattgggcg acagcaggta gatggtcgag aggttgacgt
    84421 gggccgtctt gcccttggcg tagagcgcgt cgtcgctgtt gtcagccgcc acgccgggca
    84481 ataccacctg caggttgctg accagcggcg catcccagcg gagcgaggcc tcaccggcga
    84541 agttgaacgg cccggaagcg gtggagaagc tgacaccgag ggttttgata tcttcggcaa
    84601 acacctgacg gtaggagccg agaatcggca agccggtggc cgcgaaggcg gcaccgtcca
    84661 gataggcata gaggccgctg ggcgccttgt catgatactt ggcggcatac aggccgagtt
    84721 ccaactcggt gccctccggc ttgtagcgca actgcatgcc gccctgcccg gagttcttgg
    84781 cttctatatc gctggcatgc accgtctcgt tgccgaacac ttgggtccag gaaccggaac
    84841 cttcgccaat gccatcgtta tcgctcaggt agctacccgc acccggcaac gcggagcgct
    84901 cccactcgaa ctggtagtag gcgccgaccg acagttgcgg gttgatctgc aattgcccgg
    84961 acacttgatt gaccggccgc aggatttcct tgaactgggt acccggcacg ctgagtagct
    85021 tgacgatatc gttcgggccc tgggcattgg cgatgccgtt accaccgtag aacaggctct
    85081 cgccgtaaat caggctgtgc cgaccgaggc gcagagtacc ctggctcgac tcgcctatat
    85141 tgccacgcac gaatacgaag gcatcgagaa tctcggcgtc gcggccgtgc agatcgcggg
    85201 tatcgttgag gaagtcgcgc tgcaggctgc tgtcggtgtt ctggttgtag atatcgtcgt
    85261 accaggccgc cgcactgagg cgcagaccat aattttcccg actcagatcc atttcactga
    85321 acagatccag gcgattggag accaggccgc tactgaagtt gttatcgccc tgactctgga
    85381 tattcgggta gagcccgccg gcagttggcg agttggtaat ctggtcgtcg gcaccctgca
    85441 ggcgccaggc ctggctgtac ttgatggtgt tatcccagcg cagccgccac tcactatcgc
    85501 ccaagtcaaa ttgcatggcc tgagtctggg aagcccagcc ggccagacaa accgataacg
    85561 ccagggcgga acgcttgaag cctgggtagc atcgagagtt gtgcatgttg tccctcattg
    85621 tttttgttgt tggaagcatg ttttggggta tcaccgttcg cgctagcgag cggcagtgtc
    85681 ttgcacgcaa tgcgttgtct gcggctcctc gttcagatgt aggtagacgc caggccgcca
    85741 tccacaggga tgttcacgcc gttaatccag cgcgactcat cggcacacag gaaggcaatt
    85801 gcggccgcca cctcgtcggc gtaggcaggc cgtttcatac ggtgagcgtc ggcatcaatg
    85861 cgctctttac cgagcatgct gacgaagtca ccgagaatgg gggtgaacac cgggcccggt
    85921 gccacgcagt tcatccgcac cgaggtctgc ataaaccagg gctgagcctg caggtagttc
    85981 cagacgatca aggcctcttt gaaatactgg tagcagctcg cctggggcac cgggttctca
    86041 gccagccagg cctggccatg ggcaaaactc tcggtgctgg ccagggcctt gtgcaggtcc
    86101 aagcgcagtg gccattccgc gcccaggatc gaagccacat tgacgatggc gccgccagca
    86161 cggatgcgcg gcagcacagc cccacagaga tggcgcaggc ccaggtaatt gacctgagca
    86221 accagctgcg gatcggcggt gccggggacc ccggcgatat tgcacagacc atcgacctgc
    86281 gccggcagct gctgcacggc ggcctcgatg gcatcgggat gactcaagtc ggcctggacg
    86341 aagccatcca gggtcaggct gggttcgttg cggtccacgc ctataacggt ggcaccatgg
    86401 gcacgcaggg ttcgggcagt ttcggcgccg atgcccgagg agaccccggt cacgactatc
    86461 gtcttattgg tcagcttcat acgggtttcc cgattcgtgc ttgtgctgct ggcgcagggg
    86521 cggcggcagc caaggcatat tgttattgcc gaaatagtta acgtcggtaa ctagttctag
    86581 caagcgatat gccagctcgg cgcaataatc aaacaacgcc tctagctcgc atgtttacgc
    86641 cgaatatcag gaaaatagcc tgctacaacc aggcgaacta cgctttcaaa aagtgatgat
    86701 ttcagcaaaa tcatcctgca ctatgcggaa tatgattaat ccgattaagg gttttcccat
    86761 ggcagataag ttgatttccc tggccgaact ttcccgcgcc gcgcacaagc cctccgcccg
    86821 cggtgccagc gaacagttgc ctcccggcgc cgcaccgacc ctgcaggatc ttgctgaatc
    86881 cctactgttt agccccagtg acggacgcat ctggctcaat ggcgcgcgca tgctgctgat
    86941 gcacagcagt ggcatcggcg cgctgcgccg cgagctgatc gagagcatgg gcctggcccg
    87001 cgcccgcggc atcatgctgc gcaccggcta tcactgtggc gcgcgggatg cggcactgat
    87061 cagggaacgc ttccccgagg ccgacatcct ggccgtgttc gccgctgggc cgatcatcca
    87121 tggcctcgaa ggtgcggtga aagtcgagcc ggtgcacttc gagttcgaca tgaccctggg
    87181 cacctattac ggcgaattcc tctggcatca ctccagcgag gacgacgaac atatcgccca
    87241 gtacggcatc ggcaccgagc cggcctgctg gatgcagacc ggctacgcca ccggttacac
    87301 ctcggcaatg gtcggccgcc tgatcctcta ccgcgaggtc gagtgccgct ccaccggcgc
    87361 caatacctgc cgcctgatcg gcaaacccgt ggaggaatgg gacgacgccg aggaggaact
    87421 ggccgacctt aacgccgaac ccttcgtcgg cagcagcgcc agcgcggccg cagtcaaacc
    87481 cagcgcaaaa aacgccgaca agggcgcctt gccgagcaat ctgcaagccg agccgacagc
    87541 ggcgcaggac agcgagatgg tcggcatctc ctcggccttc aatgccgcct gccatatgtt
    87601 gcggcgagtc gcgccgactc aggcaaccgt gctgtttacc ggtgaatccg gggtcggcaa
    87661 ggaaatgttc gcgcgcatgc tgcaccgcat cagcccgcgc cacgatgcgc ccttcgtcgc
    87721 catcaactgc gccgccatcc cggaaaacct gatggagtcg gaactgttcg gtgtcgagcg
    87781 cggggccttt accggcgcca cccagtcgcg cgccggacgc ttcgaacgcg ccgacggcgg
    87841 caccctgttc ctcgacgaga tagccaccct cagcctggtg gcccagggca agctgctgcg
    87901 cgccctgcag gaaggcgagg tcgagcgggt tggcggcagc cgcacgctca aagtagacgt
    87961 acgggtggtc gccgcgacca acgtcgacct gcgcgccgcc gcccagcgtg gcgagttccg
    88021 cgaggatctg ttcttccgcc tcaacgtatt cccgatccac ctgccgccgc tgcgcgaacg
    88081 caaggaggat atcccgctgc tgatgaccca cttcctccat cgctttaccc agcgccacgg
    88141 ccgccagctc agcggcttta ccccgcgcac cgccgacacc ctgctggcct acgacttccc
    88201 cggcaatatc cgcgaactgc agaacctggt ggagcgcggc gtaatcagcg ccccggacga
    88261 tggggccatc gacctggcgc acctgtttac cagcggcgaa cgcctggccc atccgatgtt
    88321 ctccatcggc gtgcgcggcc agttggccgc ggccccccac gagtcgcctc ccacccaaca
    88381 gaccagcagc acgcccccag cccagcaacc cgaaaccctg cacgccctgc tcggcgacaa
    88441 gaacctcagc cagctgtcat tgcaggaggt cgaagacgcg atgatcgacc actgcatggt
    88501 cgaggccaaa ggcaacgttt ccgaagccgc ccgccgcctg ggcctgaccc gcgcacaact
    88561 gtcctatcgc ctgtctcgcc ggccggcggg cgagtagcga atcgaccggt gcgcgcggcg
    88621 caccctacgg gaggctgtgc agccgtactg aaacccacca cgccacgtag ggtgcgccat
    88681 gcgcaccagt ctttctcgcg taaatcgcgc caggggaatt gctcgtccta tcgccatgaa
    88741 attggatcca tataggacaa gccgcccatc tcaaacatcc gccaggcaca ccacgcaacg
    88801 cttaaaaact aaatagaacc gcttaaatcg gccgataatc gaccacaaaa acacctgaac
    88861 gcagactttt gctttgacac ctaggcacac caacgagaat cattggatcc aataaaaaca
    88921 accaaggccc actcaggaga gcctccatgt tcccgaaaaa cacctggtat gtagcctgca
    88981 ccccggacga gatcgccgag aaaccccttg gccggcagat ctgcggggag agaattgcct
    89041 tctatcgtgg gccgcaaggc aaggtcgcgg cggtcgagga tttctgcccg caccgtggcg
    89101 cgccgctgtc cctgggtttt gtcgaggacg gccagctgat ttgcggctac cacggcctcg
    89161 ccatgggtgc caacggcaag accgcggcca tgccaggcca gcgcgtgcgc ggcttcccct
    89221 gcatccgcag ctacccagtg gaagaacgct acggctttat ctgggtctgg ccgggcgatg
    89281 ccgagcaggc cgacccggcg ctgatccatc atctggaatg ggccgacagc ccggactggg
    89341 cctatggcgg cgggctgttc catatcaact gcgactaccg cctgatgatc gacaacctga
    89401 tggacctgac ccacgaaacc tatgtgcacg cctccagcat cggtcagaag gagatcgacg
    89461 aggtcgcccc ggtgaccacg gtcgagggcg acaacgtggt caccagccgg catatgcaga
    89521 acattatggc cccgccgttc tggcgcatgg ccctgcgcgg taacggcctg gcagacgatg
    89581 tgccggtgga ccgctggcag atctgccgct ttaccccgcc cagccatgtg ctgatcgaag
    89641 tgggcgtggc ccatgccggc aagggcggtt atgacgcagc cccagccgac aaggcggcga
    89701 gcatcgtggt cgatttcatc accccggaaa ccgagacctc gatctggtat ttctggggca
    89761 tggcgcgcag cttcaagccc gaagaccagg aactcaccga cagcatccgc gaaggccagg
    89821 gcaagatttt ttccgaggac ctgcagatgc tcgagcagca acaggccaac ctgctggccc
    89881 acccagagcg ccagctgctc aagctgaata tcgatgccgg cggcgtgcag tcgcgacgca
    89941 tcctcgaaca cctgatcgcc caggaacgcg cgccagccgc gacgcccgag ctgatcgtca
    90001 gcaccgccgg tgcaaggagc gccccgtgat cgaagtatca gtagtcgccc gtaacaacca
    90061 ggcccaggat atctgcagtt tcgaactggc ccgcctcgac ggcgcgcctc tgccggcatt
    90121 caccgctggc gcccatatcg acgtgcacct gcctggcggc ctggtccgcc aatactcgct
    90181 gtgcaaccca ccctgggaaa accagagtta cctgatcggc gtgctcaagg accccgcctc
    90241 gcgcggcggt tcccaggcca tgcacgaaca gatgcaggtg gggcagacgc tcttgatcag
    90301 cgagccgcgc aacctcttcc cgctgagcga gaatgcccag cgcagcctgc tgttcgccgg
    90361 tggtatcggc atcaccccga tcctgtgcat ggccgagcgt ttgtaccagc tcggtgccga
    90421 gttcgagttg cactactgcg cccgctccat tgaacgcgca gcctttgtcg agcgcctgca
    90481 gaccgcgccc ttcgccgaac gcgtgcacct gcatttcgac gacggcgatg ccgagcagcg
    90541 cctggatgcc gccaacctgc tggccaatcc caatcccggc acgcacctgt atgtctgcgg
    90601 cccgggcggt tttatggagc atgtgctgag cacggccaag gcccagggtt ggccagacga
    90661 acgcgcacac cgcgagtatt tcgccgccgc acccatcgat cacgccaacg acggcagctt
    90721 cgccgtgcag ctcaacagca gcggccagat catccagatt cccgccgacc agagcgtcgc
    90781 cgaagcgctg gaagcgtccg gcattctgat cccactgtcc tgcgagcaag gcatctgcgg
    90841 cacctgcctg acccgtgtac tggccggcga accggaacac cgcgacctgt tcctcaccga
    90901 agccgaacag gcacagaacg accaattcac cccctgctgc tcgcgggcga agagtccgtt
    90961 gctggtgttg gatttgtagg gcggacatgc cgaaggcttg tccaccatcc gggccaccta
    91021 cctcgctctg gtagcccgga tgcaatccgg gaaaatctcc gccgcggcaa caaatttccc
    91081 ggattgcgct ggcgcttatt cgggctacga aacggtagca gccagcctgc atcgacaccc
    91141 gtaggagcgg gccatgcccg cgatgctctt gtgcccgtat cgagggcatg gagctaggca
    91201 taatcccaac gcttacgacc gctcttctgc cgcgcccttc tgaatcacct tcaccccttc
    91261 actggccccc tgggtcgggc cgaagatttc cgcgtagcgc agggtcgcgt tggcgtgctc
    91321 gcgcataatt gcctcggccc gcgagccctg accgttgact agggcgtcga acaccgcatg
    91381 gtgctgcata tgggcgaaat tgaagcggcg gtattcgcgg gccatatcgt gcaggtcgac
    91441 cgccagcgag ctgaccgagg caaacggcag gtggtcgttg cgcgccaggg cctcggcgat
    91501 ggccggattg ccgctggcgg cgatgatcag ctgatgaaag cgcatattga ggtcgtggta
    91561 gctctccaga tcctcttcca gcacatggcc cttctgaaac agtcggtcac cctgctccag
    91621 gcattgctgc aaagcctcgc gtacctcggc gttgatgccg cgttccgccg cctggcgcgc
    91681 ggccagaccc tcgagcaccc cgcgcacctc caccgcaccg gcgatatcgg ccgggctgac
    91741 cgcacgcacg gtgtagccgc gccctgcgca tttgaccagc agcccctcct gctccagggt
    91801 acgaaaggcg atacgcaccg gcgtgcgcga cacctcgagc aattcggcgg tgggaatctc
    91861 ggcaatgcgc tcgcccgcgg ccagctcccc ggaggcgatc atcttgcgca gcgcgatcag
    91921 aacgcgctga cccggcttac tcataactgt ccccagccag ttcccaatgc gcgccatcat
    91981 agcgcagcca gggcgcgatc aacccagcgc cccgcgggcc ataaaacagc ggctgaacat
    92041 gcccagggcc acctcgaccc gttcgccgat ctgctcgtcg gaccagctct gctgcacccc
    92101 cagggtgaac agtttgagcg gctgcgagat cagcagcgcg tcaatcagct cgaccgctgg
    92161 ttctacatcc tcgcgcgcca gcaggccctt gcccgcggcc ttgcccagcc agccacgcag
    92221 caggttaagg caacgctcgg caccctcctg ataccagatc cgcgcgatac ccggcgcatt
    92281 ctcgcggccc tgcgcagcca tcaggtagat gcccagggcc aacggcgaca gggtcaggcg
    92341 cgcccacagg tacaggtaca tacgcagctc gctaagcagg ccctcggcac agtcgatgcc
    92401 gctttccagc agcaactcga gggtcgacag gtcgcgggcc accagctgcg acatcagctc
    92461 ttccttgctg gctaccagtt gatagagggt tttcttcgaa atgcccgccg aacgcgcgat
    92521 ggcgtccatg gtgacattgg caaaattgcc gctgccgatc tcggtcgccg cggcgtccag
    92581 cagcaggctc atctgctcat cgcgactgcg cagcggcggc cggccgcgtc gggggagctg
    92641 aggggtggtt tcagacataa gaaattctgc tgatcggcat gacttgggat cctggcggcc
    92701 agcgccatgg agtgccgcgt agcttaccgc ggcataccgg taaacgccgc gtcgatcgaa
    92761 ccgcccggta tgcggcctga atgccaatat cctgaatgcc gaaatgtagg agccagggtg
    92821 tccccggatc taggccaacg cacccgcacc tctcacctcg gccactgcct caagctgaaa
    92881 cgcctcacgc aacccatcct ccatcaactg ccaagcctgc tccgccgagt cgtccggccc
    92941 caggtgggta aacatatggt cgcagccggc gaacatccgc tcggtcaccg tcaccccggc
    93001 ctggcgcagc ttctcggcgt aggcttcacc ttcaagccgc aggatgtcgt actcggcggt
    93061 aatcagggtg gctggcggca tgccacgcag ctcgtcaacc gccagcagca acggcgaaac
    93121 cagcggatcg cgggcctggg ccggatcgct caggtagcag ctattgaaga agcgcatcag
    93181 gccgacgccg agcaagggct tgtcgagagt cgaggtcttc aactctggtg cctgactcag
    93241 atccagcgcg gcatagtcga tcagctgatg caccacccgt agccgttgat gcccacgcgc
    93301 caggttggcc acgccggtgg ccaaattacc gccggcgcta tgcccgccaa cggcgatacg
    93361 ctgcggatcg atgcccagcg tggcggcctg ttcggccagc cattcgagca ccgcaaagct
    93421 ctggcgcaag ccggcgggaa agggatgctc aggggccagc acataatcga cattgaccac
    93481 caggcagccg aggttatgcg ccaggcggcg acaatagctg tcgtcgtgct cgggcacccc
    93541 ggcgacgaag ccgccgccat gcaggttcag gtacaccggc aagggctgcg ccgactctgt
    93601 gttggaccag tagaacagcg cccgcgccgg cccccaggca gtcgggacat attcctcggt
    93661 caccttacgc aacggatact gcgccgcgtc gtagacgaat ttacctttca tgcgtttggc
    93721 aaaggcgcgc agcagcttgg ccttaaggtt ttttagcaac ggcataatcg gactccttaa
    93781 cggctcaatc aattagcggt cagcagctgg tccagcagcc catgaatcat ctcgccataa
    93841 ggcgcgcgca tcgccaggtt ggactcgcca atcgggctct gcaccaccac cgccttggcg
    93901 tggctaaagg tgcggaagcc atagacgcca tggtaggccc ccatgcccga gtggccgacg
    93961 ccgccgaagg gcagttcttc gaacagcaca tggctcatgc agtcattgat caccactgcg
    94021 cccgaggtgg tgcgttcgag catctgctgg cgctcgccgg catcctcgcc gaagtagtag
    94081 gccgccaggg gccgtggctg ctggttgaca tggtcgatgg ccgcgctgaa atcgcggtag
    94141 gtcttgatcg gcaacaaagg accaaagatt tcctcgcgca gcacctgcat ctgctcgttg
    94201 gtatcgagta tcagggtcgg ggcgatcttg cgagtctcgc ggtcgctcag gtcttcctgc
    94261 gccgggttga tctcgaccaa gtacgcgccc ttggcgtggg catcggcgag gtagctgcgc
    94321 aggcgatcga agtggcgcgg gttgatgatc gaggtgtagt cggggttgct gcgcagggtt
    94381 ggatacatct cgctgacaaa acgcacggcc tcggcgacga agtcacccag caactcctcc
    94441 ggcagcagca gatagtccgg ggccaggcag atctgcccgg cgttgaaggt cttcaccgtc
    94501 atcacccgct gcaccacggt ggccaggtcg gcgctgcgtg agaccagcac cggcgacttg
    94561 ccgcccagct ccaaggttac cggcaccagg ttgtccgcgg cggcacgcat gataagccga
    94621 cctaccgccg tgccaccggt aaagatcagg tgatcgaagg gctgtgcact gaacagcgca
    94681 ccaacctcgg cgctgcccag tacggtgctc agttcgttag catcgaagta acgggcgatc
    94741 agttcagcga gcagcgccga ggtgcacggg gtcagttcgg agggtttgag catggcgcgg
    94801 ttgccggctg cgaagatgct cgccagcggg ccgaaggcca ggacgatggg gaagttccac
    94861 gggctgatca ccccgaccac gcccaacggc tggtagtcga cccgcgcttc gcagccgggg
    94921 aacggtgcct ggtgggcatc ggactgcatc cactcagcca agtgctcgcg gcagtgcttg
    94981 aggctggcca ccgagccggc gatatcggac agcagggtct gctcacggct gcggttgccg
    95041 aagtccgcgg ataccgcggc gactatctct tcacggttat ccagcaacag ggcgatggcc
    95101 cggtcaaggc ggtcgcggcg cagctccagg ctggccgggc cctcggccag atgggcctgt
    95161 ttcatggcgg cgagggccgc agcgagttgt tcggcgctga ccgccgcagt ctggtgaccg
    95221 agaatgctca tggggtaatt ttcctgctgg ttgaattgtt attagccagc actaagtgct
    95281 ggaccagccg gcacctctgc cgactcgatg gctggagtat ggcaaaaaac acaacaaagg
    95341 aaactaccag gtttccttat ttttaaattt ctgactaaag gctctacccc ttcaaacaag
    95401 gggccattga atagtcctgg gcaataccac gcccgcccat ggatttcccc gaaacgccgc
    95461 gggcggtttt agcaactggc caaaccctgg ctattggtgc gaaactgatt aggcttatta
    95521 accagtatcc atttcggcaa cccctagcga gcacccggtt ccagccaatg gccaagaaaa
    95581 gcccccccac cccagaagtg aacagcccag ccaccgagcc gagcgacacc ctgctcagcc
    95641 agctgatcgg ttacgccctg cgccgcgcgc agctaaaggt tgcgcagaat ctggtcgagc
    95701 aactcaaccc cttcgacctg cgccccgccc agttctccgc cctgctgatt atcgaggcca
    95761 gccccggcct gatgcagacc gacctggcga gcatcctcgc catcgagcca ccgcaggtag
    95821 tgacgctgct aaacaagctg gaaaaactcg gcctcgccgt gcgcgtgcgc tgcaagccgg
    95881 acaaacgctc ctacggcatc ttcctcagca aggccgggga aaccctgctc aagcaactca
    95941 aggaaatcgc cgccaccagc gaccgcgaag ccaccgctgc gctcgatgag ggcgaacgcc
    96001 agcagctgct gcagttgctg cacaaggtct atcggcagga cggcaatggc acggattgag
    96061 cgccgcccct cggtgcgcac ggcgcaccct acaaaactga tcgtcgagcc tcgctgatat
    96121 gcgcccaacc cttgggagag actgggcggc accccgcttt agccgcgata acaacgattg
    96181 aaagctcgcc gctaaagcgc ctcccacgcc gttgcgccgt ggcgaagctc gcggccaaaa
    96241 cccacgctct gttaaccccg ctcgaacgcc ggattctcga gcagcaccgc cataccctga
    96301 ccaccaccga tacaggcggc ggcgatgccg tagcgctggt tggctgcgcg caactgtcgg
    96361 gccaggctca gggtcaggcg caagccggtg gcggccagcg gatggccgag ggcgatggag
    96421 ccgccttgcg ggttgaggcg ctgcgggtcg agctgcaatt cacgctgcac cgccagtacc
    96481 tgggcggcct gggcttcgtt gatttcaaag cgggcgatat cgtccagacg caagccgctg
    96541 cgttccagca ataggcggat cgccggggcc gggccgatgc ccattaattc cggtgccacg
    96601 cccaccaccg cagtcgccag caagcgcgcc aatggcctgc gccccgctgc cgcgctggcg
    96661 gagccgacca gggccgccgc cgcgccgtcg accaccgcgc aactgttgcc ggcggtctgc
    96721 acgccgccgg catgaatcgc gcgcaggcgt gagagcgccg ccagactggt cggccgcgga
    96781 tggctgtctt gggtgagcga ttcgacccca cgcggcaaga cgactccacg gggttgatag
    96841 ccgtccagcg cgaaggattc gctgtgcaca ctgacaatct cgtcggcgaa cagaccctcg
    96901 ttctgcgccg ccagggccaa cgcgaaactg cgcaaggcgt aggcatcggc ctcctcgcga
    96961 acaaccccat aacgccgcgc caggttttcc gcggtgccga tcatgtcgat atccgccgcc
    97021 gggtcgaaca gcgcctccca gaggaaatcc ttgaactcca ccccagcacc gaggcggaaa
    97081 ccgccgcggt gggtataggc ggcgataggg ttgcgtgaca tcgactcggc cgcgacgcac
    97141 agggccaact cggctctccc ggcggcaatc tgctcgccgg cctggcgcag cagctcgaaa
    97201 cctgtaccgc agatgcgctg cacgcccaag gccggcaccg tctgcggcac gccgctgtac
    97261 aggccgatat ggcgcggcag catataggca tcgaaactcg cctgggccac gcaaccggcc
    97321 aataccgagt ccaccgcagc tggatcgatc tcggcgcggc gcagcacttc gcggccgacc
    97381 ttgatcccca ggtcggtcgg agagaccaaa cccagggcac cgccgtaatc gcaccagggc
    97441 gtgcgcacgg cctcgagcat cagcacatcg tcgaacgccg aatagagacc gccggccatg
    97501 cttagcgctc cgccgttaca cgttgcggat ctgcgccctg gtagagggcg tcgatgcgct
    97561 cggcgcggca actgaggatc acccgctggt tgagcgagcc cttgtcggtg acctcgccct
    97621 tgtccagcga cggcggttcg ctcatcaaac aggcccaggc caggcgcgag gcgctgccgg
    97681 tggcctgttg gttgagctgt tcgagcagct cactcagcca ggcccgcacc tgcggcgcgg
    97741 ccaacacctg ctcggccgtg gccttgggcg acaaaccggc cagctcgcgg caggccgcca
    97801 gctgcgggaa caccagcagg ccgatacatt cgcggttcgg cgcggtcacc accacatcct
    97861 ggatataggg cgcgccggcc atgatcaccc gcgtgcgcaa cggaccgacg ctgacgaaca
    97921 ccccggtgtt gagcttgaag tcctcggcga tgcggccgtc gaacatcagg ccccattccg
    97981 ggtgctcggg gtcagccagc ttcagcgcat cgccggagca gtagaagccc tcctcgtcga
    98041 aggccgcggc gctctgctcg ggcgagcgcc agtagccggg catcacatgc gggccacgga
    98101 aacgcccttc gagcttgccg tccaccggca ccagcttgac ctcgcagccc ggtgccggca
    98161 ggccgacata accggccatc gacagcggcc cggtggtaaa ggtgcaggac ggcgaggtct
    98221 cggtcatgcc gatgcccgcc atcatgcgga tgcgctcgcc acagtgcgcc tcggccaccg
    98281 catcgaggcg atcccagacc gcctgcgaca gcccggcacc ggcgaagaaa aacagcttga
    98341 tttgagcgaa gaaggtttcg cgcagctcgg catccacctc cagggcggcg accagctctt
    98401 cccagccctt gggcacggtg aggtaggcgg tcggcgaaat ttcgcgcagg ttgcccaggg
    98461 tctcggcgaa atgctgcggc gtcggcttgc cgtcgtcgat atacagggtg ccgccgttgt
    98521 acaggacgat gccgacgtta tggctgccgc cgaaggtgtg gttccacggc agccagtcga
    98581 ccagcaccgg cggcgcctcg ccgaacaccg ggaaggtctg caggagcatc tgctggttgg
    98641 cgcagagcat gcgctgggtg gtgatcaccg ccttgggcag cttggtcgaa ccgctggtaa
    98701 agaggaactt ggcgatgctg tcggggccgg tcgcggcaaa ggccgcatcc gccttatcgc
    98761 aggcctcatt gccaagcagg ctggcgaacg agtgacactc gcgccccgcc acctcgcccg
    98821 cggtcagcag taaaggcgtg tcagccagca ccaccgcctc gatagcgcgg gcgaaggcct
    98881 ggccatcggc ggcgaacacc agccccggct gcaacagcat gcaaatatgc tgcagcttgg
    98941 tgtagtcctg ggccaccagc gaataggccg gcgacaccgg gcaatagggc acgccgagat
    99001 acagggcagc gcaagccacc tgcagatgct ccagatcgtt gccggagaga atcagcagcg
    99061 gccgctcgac cgacagatcg aagccgagca ggccctgggc gatactgcgt actcgcccga
    99121 gcatctcggc atagctgatg cgctgccact ggccatccgc accgcgccgc gcaataaagg
    99181 tctgttcggg acgctcctcg gcccagcgca gcaggcgctc gagcaggcgc tgcggatacg
    99241 cctgcagcgg ctccaacgct tccatataca gcgcctgccc gacctcacgc agttgcaccg
    99301 cagacttgcc gatcgataca ggtcggtaga cgggttcgac gcgaccgcta gattggctat
    99361 tcacctgtga aactcctcag caaagcattc ccggactgcc ggccgcccca cttcatggag
    99421 gcgcgaccgg gcccgagctg attgttcttg tcgtgctgct tgcctgttaa ggatctgcgc
    99481 agcagatcct gtgcaggttt tcagatcggg taatgacgcg gcccgttctg aatagtcacc
    99541 caacgcaact gggtgaagaa atcgatcgac gccttgctgc caaagctgcc gtaaccgctg
    99601 gccttgactc cgccgaaggg catctgcgcc tcatcgtgca cggtcggacc gttgatatgg
    99661 cagatgcccg attcgacccg ttgcgccaac aacaaggcgc gcgacacatc gcgactgaag
    99721 atcgccgccg acaggccgaa ctcggaatcg ttggccaggc gcagcagctc ctcgtcgccc
    99781 tcgccgcgca gcaccaccgc caccggacca aaagactcct cgtggtacag gcgcatatcc
    99841 cgggtgacac catcgagcaa agtcgcctgc atgatgctgc cgtccagctg gccgccagtg
    99901 accagacgcg cgcctttggc cagggcatcg tcgaccagcg ccttgatccg ctgcccggcc
    99961 gacacatcga tcagcgagcc gagcaccgac aggctgtcac tcggatcgcc ggcatgcagg
   100021 cttgcggcct tgctcgccag cttatcgaca aaggcatcgg ccaccttggc atcgaccacc
   100081 agccgttcgg tggacatgca gatctgcccc tggttgaagt aggcaccgaa ggctgccgcc
   100141 tccactgccg tatccaggtc cgcgtcgtcc agcaccagca agggtgcctt gccgcccagt
   100201 tccagcagtg ccggcttcag gtgccgcgcg gccagctcgc cgataatccg cccgacatgg
   100261 gtggaaccgg tgaagttgac acgccgcacc gccgggttgg cgatcagccg ggcgacgatg
   100321 gccggcgcgt cttctggcgc gttagtgatc acgttgacca cgccatcacc gaggccagcg
   100381 tcctgcagca cctggccgat caagcgatgc accgctggac tcagttcgga tgccttgagc
   100441 accagggtat tgccgcaagc cagcggcatg gccagcgccc gagtgccaag gatcaccggc
   100501 gcattccagg gcgcgatgcc gagcaccacg ccgcagggct gacgcagggc catggcgaaa
   100561 ctgccgggca cgtcggaggg gatcacctcg ccatatatct gcgtggtcat cgccgccgcc
   100621 tcgcgcagca tgctagcggc caggtgcacg ttaaagccgt accagttggc catcgccccg
   100681 gtctcgccgg ccaccgcgag gaattccggc gcacgatctt cgagcatctc ggcggccttg
   100741 agcaggcgcg cgcgccgctc gttgggtgcc aacgcggccc aggcgggaaa tgctgcctgc
   100801 gcagcagcca cggcggcatc ggcgtcttcc agggtggcgg cagcgacttg cgacaccact
   100861 tcgccggtca ccggattgca gcgctcgaat acgcgaccat cggcagccgc gcgcgactga
   100921 ccgccaatca gcaaaggcac ctcgaacatg gggaattcct cttattgtgc ttattctgga
   100981 agcgcgccaa gcctgctcag gcctggcgcc aggttagcgt tttagcgctt gtaggcctgc
   101041 aagccgggct tgatgctctt ctcgtcgagg aactgcttca tgccctgttc gcggccgcct
   101101 tcggtgtcga gcagacgcga ctggtcgagc ttggcgtaca ggtaatcctc gttctgctcc
   101161 caggtcagtt cgcggcagcg cttgaagccg atcttcgccg cacgcatgac caccgggttt
   101221 ttctccagca gattgcgcgc cagctcgatg gtggtttcgc gcagctgcgc cagcggcacg
   101281 ctctcgttga ccagacccat ctgcgcggct ttctgcccgt caaaggtctt gccggtcatg
   101341 atgtagtaca gcgactggcg gtgaccgacg gtatcggcca tggccttgct caccaggttg
   101401 cccggtggga tgccccagtt gatttccgac aggccaaagg tcgcttcgtc ggcgcagatc
   101461 gccagatcgc aagccaccag cgggctgaag ccaccaccga agcaccagcc gttgaccatg
   101521 gcgatggttg gcttggtgta catacgcagc agcttccact gccattgcga ggcctcgcgg
   101581 cggatcttct cctggaggat ttccgggccg gcgtcgattt cgcggaagta ttccttcagg
   101641 tccatgcccg cagtccaggc ttcgccggcg ccggtcagta ccagcacgcc aacgtcagga
   101701 tcctgctcca gagtctccag cacctcgacc atttcgcggt tcagggtcgg gctcatggcg
   101761 ttgcgttttt ctgggcgatt gagaatgacc caaccaatgc cctcttcaac ctcaaccttc
   101821 accgtctgcc agcgattctc gtagctactc atctcatacc ccttcttatg gttgtcagca
   101881 cctggagaca aaattatctc gcaaatatag ttatgtcaat taactatatt tcataaaaat
   101941 aagtattttg atcacgcaag gcgccatgag cgcacgtaca gagtgaggaa tagcgagtaa
   102001 aaaagacaat tcagcataaa tcgcaaaaaa ttacgttaac gacaataatc gttatgttga
   102061 gcagaaattg atcagcccac cctgatgtgc tgcctagaca ggcgcaaaga aatacctgcc
   102121 tgcacccgac cattgctgag gtgaattggg cctccctcta cgtgacacag aaacctaatc
   102181 gccacgctag cgacgttcct caccgacgcc actgaggcgc ctccaataac tctcggtaag
   102241 caaacagtcc tgcggctaga gcaaagcctg tagctctagc tggcatgcag cgctgcagac
   102301 aggcaaacgc atcgaataaa actccgcctg tcccatctgc tgccttttgt ggccgtaaac
   102361 cgctacaatc gcgccccttt cgcgcccgtg cgcccgttta tcctggacca agattcgtga
   102421 tctccaccgc caacatcacc atgcgagaaa ttgtgtgttt gtgtgggtac ctgtgcgtag
   102481 gaaacatgcg gttttcagcc acttgagtac ccagtaaaac tcagtgattg gtatcaaaat
   102541 cggtatcgga tttggttact tcctacccat ttcgattatc tcgactgatc acccttgccc
   102601 agctcacgcg ggcagcaaag ctgtatttgg aaccctcagc caacctctcg acctcgactt
   102661 gcagttttca aaatgaatag acactggaac tgccagacca atctggctac ccacgcaagt
   102721 gagccttcca gtcgaggggc aagtcatgct gaatactctg atcgccaaac tcaccacatc
   102781 cctgctccgc ccgaaattca atcctggcta ccagaccagg gtttatccgc gattgggtga
   102841 gatggtcatc gtccatgact cgctggactt tggccccatc gcgacagcag acgagctaaa
   102901 gccgatcgac gatttctggc taaacgatga aatgcctatc accggcctgc tcacccatag
   102961 gcgctgaccg ccctttcgtt tactacttct tggtccatcc cgcccgcctt taagtccatg
   103021 agtcagaggc aagatgctac tcgcaagacg agcatcaata ttgccgtctc cgctctgtta
   103081 gctcatgtgc aggtatcgtt ccagacaagg cgtgcaagga cactgccgat ttgggctaac
   103141 gcaccgtctt gcgtgctttc tatagtggct gcacagccgc gcggcgaaag caccacccgc
   103201 cttttgcgcc ccgataaaag aacaacaatc cgaggcctga aaccatgatc cgtggcagca
   103261 tcgttgcact cgtcaccccc atgagcgaaa acggcgcaat tgactactcc gccctgaacc
   103321 gtctggttga ctttcacttg cgcgaaggca gccacggcct cgtcgcagtc ggcacaaccg
   103381 gtgaatgctc ggcccttgat ttcaaggagc acctcaacgt catacggcac atcgtcgagc
   103441 gcgttggcgg acgcttgccg gtaattgcgg gtaccggcag caacagcacc cgagaagcga
   103501 ttgccctgac agccgcagct gccgatgctg gcgcagatgc ctgcctgctg gtcacgccct
   103561 actacgtgcg cccgactcag gaagggctgt atcagcactt cgcagctatc gccaaggccg
   103621 taccgatccc gcagattctc tacaacgtgc cggcacgtac tgcctgcgac ctgttaccca
   103681 acacggtgtc ccagctggcc gaactcgaca ccatcgtcgg catcaaggaa gccacggggg
   103741 atctggtccg cgcccgggcg ctgctgccac tggctgcaga cggctttgcc ctgtactcgg
   103801 gcgacgacgc cagcgctgcg gaattcatgc tgctgggtgg ccagggcaac atttcggtca
   103861 cggccaacgt cgccccgcgt gccatggcca agttgtgtga ggccgcattg gccggggatg
   103921 cggcgcaagc ccgcgcccta caacgttact tacaaccctt gcatgccgcg ctgggtgtgg
   103981 aaaccaaccc tatcgccgtg aagtgggcgt tacagcggat gaagctgatt ggtgcaggca
   104041 ttcgtgcacc actgacctgg cttggccacg ctcacgaagc cctcctctgc caagccatgg
   104101 acgactgcct catcccctct cagccctgac atccaacatc aagcatcact cgaggttcga
   104161 ccatgtcttt tgaacgcata agcattcagt ggcgcatcag cctgcttagc ggttgcagcc
   104221 tgctcgccgt cgtcgccgtg gtactcggca tcaccctgta tcaagcgcac cgtaacgccg
   104281 agctgttcaa gcgcgagagc acgcgcatcc ttgatgaatc ggcgcaggca cgacttgaag
   104341 ccagcgcagt ggcgcagaaa ctgcgtatcg agcacttctt tcgcagcatg gagttgtacg
   104401 gcgacggcat cgccgggcag gtgctgcaat tgcgcacgca gcacgcgggc ggtgccggtt
   104461 cggccgatac gctgcgccgg caactggtcg cactgatcca tgacgccctg cttgccagcc
   104521 caaagacgct gggtatctat ctggtgatgc agcccgacgc cctcgatggt caggacagcg
   104581 acttcatcgc ccaggagccg atgtcgagca atgagaaagg ccgcttcgcc ctgtactggt
   104641 cgcaaagcga acccggcaag ctggtgcagg caacactgac cgaggaaaac atcaacgcca
   104701 acccgacgcc cgccggcgca gagccgcaga acgcctggta cgtctgcccg gtacaaagcg
   104761 gaaaaccctg cgtggtcgag ccctatgcgg tagaggtgga aggccgcagt acgttgatga
   104821 gcagcctgtc cattcccctg attgataacg gccgggtgat cggcgtgctg ggtttcgaca
   104881 tcagcctggc caccctgcaa cgcctgaccg agacgctcag cgccgagctg tatcagggtc
   104941 agaacgccgt ctacattctc tccgccactc acctgctggc cgggcacagc gcccagcccg
   105001 acaagctggg taacccgttc gccagcgcgc ccacccccga cagcgtgcag ctgagccagg
   105061 tcttgcagcc cctggccgat tcgcggccct ggcacctgca tatcgatatg cccaacgcgc
   105121 agctgcacgc ccccgcgcaa cgcctgcaga cgcagatgga caacggccag caggatgcgc
   105181 tgtggcaaag cctgggcctg gggctcggcg tcagcctgct tggcgtgctg ctgatcggcc
   105241 tgagcgcacg cagcatcagc cgcccactgc aacgcgtggc cgagatgctc gacgaactgg
   105301 tggagggcga cggcgatctg acccgacgcc tgcccaggca gcgcagcgat gagctgggcc
   105361 ggctggtcac cggtttcaat cgctttctcg accacctaca acccatggtc ggcgagatcc
   105421 agagggccgc cctcggaacc cgcgaaacag ccgaccgagc ggccggcgtc gccgaggaca
   105481 tcaacgccgg catgcaccgc cagcaccagc aggtggagca agccgccgtg gccctgcagc
   105541 agatcaacgc cagtgcccag gaagtcgccg gcagctcggc cctggccgcc gacgcggcgc
   105601 gggccgccga ccaggccggc cagcaaggag tgctgtcatt cgccaagacc acagaaacca
   105661 tcacccagct ggatcagcgt ctcgaagacg ccatgcaaga cgtgcaagcg ctggccggca
   105721 gcagcgagga aatcggccaa gtactcgatg tgatctgcac catcgccgag cagaccagcc
   105781 tgctcgcgct caacgctgcc atcgaggccg cacgggcagg agaccagggc cgaggcttcg
   105841 ctgtagtggc cgatgaagta cgccacctgg cggacaacac gcagaactcg gtgggcaaga
   105901 tccgtctggt ggtggaaaac ctgcagcagc tcagccgtga cgtggtcagc ggcatgcacc
   105961 gcagccgtga gcaggcgcgc aacagcgtcg gcctgctcga cgacacccat accgcgctgc
   106021 acaccatcgg cgaagccatc agcgtgatcg accgcatgaa ccggcaaatc gccgaagcct
   106081 cccaggcgca aagcgctggg ttggaggacg tggcccagcg tgtcgatggc atccgcagca
   106141 tcagcgcgac actcacgacg cagattgagg cgtcatcgca tatcggccga tccctgcacg
   106201 gtatgtctat ccaacaacag cgtctggtgg agcacttccg cacctgactc gcccacccaa
   106261 aggagccacc ctatgagacg tctgctctat ccgctgctgc tggtcgccgg cctgcaggca
   106321 caggcccagg acgtgattcg cttctacagc tggaaagatt atttcgaccc cgcggtgctc
   106381 aaggacttcg aagcgcagac cggtatccgc gtcgactacc aggcctacac caccgtacaa
   106441 gagctcaatc aggcgctgcg cgatggcgaa gcctatgacc tcatcgtgcc gagccacttc
   106501 atgctcaaac agttgatcgc cgagcagcgc ctgacccgcc ttgatgtccg acgcctaccc
   106561 agttactcgc agctggatcc ctggttgctc tcaatgctcg ccggtatacc agcagccaac
   106621 cagtacagcg tcccctacct gtggggctca attggcctgg cgataaatac ccaaacagct
   106681 caggccatct atgccgacgc cttgcccaac agttgggcac tgctgttcga tgaggcacat
   106741 tcagcccagc tcagcgcttg tggtatcggc ctgccggacg cccctgaaga ggtcacatcc
   106801 gtgctgctca actaccaagg gcgtcgcctg tccgctagcg gaaacaggca gatcgcccgt
   106861 agcgtgcaaa cactcacccc gcttgcacct cttctacgag ctctggacaa ctgggagcat
   106921 atcgacgcgc tggcggcagg ccgcctttgc cttgccatga cctggagcgg tcacgccctc
   106981 aaagccatgc acgacaatcc gcgattgacc tatcgcatcc cgcaggaagg cagtgcgatc
   107041 tacatcgata ccctggccat tcccagcaat gccagccaac ccgagctagc ctaccggctg
   107101 atcgactttc tgatcgcacc ggacaacatc gtccgcaatg ccctcgccac caagttctat
   107161 gccccgctgc ccagtgacgc cactgcgctg cagacgctga tcgaggaaaa cccgctgcag
   107221 gtgctgagcc cggcgcagcg gcgccggagc tatctgctgg aaagcctgct gccagctcag
   107281 aagcagacgc tggaacagag ctggcagcag ttcaagagtg ccgccagcaa ggtgcagccg
   107341 acaccctgag ggcgagaagc agcgctaggc gcgccgcgtt tcggaggggt attcgccgaa
   107401 cagggcgcgg tagtgagtag cgaagtgact gaggtggtag aagcccatct ccgccgcgac
   107461 ttcgccgacc ttgaactggc tggccggcgt actgatcaag cgcctgcgca ccgcgttcag
   107521 gcgcaggttg cgcaggtatt ccaccggccg cgtgccggta atcacctgga agctgttctg
   107581 caaggtgcga cggctgacgc gcagctgctc gcagaggtca atgatgctga tcggtgaatc
   107641 aacgccagct tccatcgcca gttcctggct acgcttgacc agataggcgc agactgccga
   107701 gctaccacgg cgatcacggc cttcatctga ggcattactg aatacgtcga gaaagacatc
   107761 aagcagagtg tcttcgagca ttttctcgac tgccgcgttg accggttcgg tgcgctgcag
   107821 cagtgactcg aagaccggat agatacgctg acgcacacgc tgctgcaccg gctcgctgag
   107881 cgacagctgg ggcatcgact tgagcagttc gatcttgttt gaagaaagct ccagcgcggc
   107941 cagtttggcg aagcgcacca gatcgacatt ggcaccgaga aagcacaggc cttccggcga
   108001 gtgcaggaga aactcttcac cgtcgcgcaa caccatcacg ttatgcgcac cgaccttctg
   108061 cccaagcacc agcggtgagc gatcggtact catcagcaag gcaatgcaca gccgcccctt
   108121 gggcgaacgg ccatgctgca cggtgcgctt gcccaggctc tcctggaata tctggaaatg
   108181 gtcggccacc acctgccgca gctcactgga aaagcttccg caactctgct gatcgtagac
   108241 ctgctgccag cccaggatag aactggcatg ttgctgcgga tcggaaaagg aacgaatttc
   108301 gctgaacatg actatcgcct ctctgcgcta ttcaccgcca ggaagagtca tccctgaacc
   108361 gctacgcgct ttcttaactt atttattcga agcgtactac caactctact cccatcatca
   108421 gcaggcggta ttccccctgc tgaatatcca ctggcgctaa cagcgcacca gagagaatca
   108481 cctgcccagc gcgcagcggc tggtcgtcgg ccaacagtcg gcgcagcagc catagcaggt
   108541 tctgctcagg ggcctcgccg gcctcggtgc taccaagcac agcgtcttca tgcagcagac
   108601 ggcagccgat ctgccggcaa gcctgtgcgt cgaatgcctg ccagggcccc aggcaataca
   108661 atcctgctgc cgcatcgtca gcgagaaagg cgccaacgtc gaaacgccag ccctgccagc
   108721 ggcagtcggc gatctcgaag gccggcgcca tacgggtcac ggcggccagc agcgcctcat
   108781 cctcatgtaa gccgggtgca atatcgtggc caagctcaaa ggccacttcg acctccagct
   108841 tgggagcgat tagctgcgcc agcggtaata cgctaccggg gctacgcgcc atatcgacac
   108901 tcagccaacc gtagacaggc tcacgcaggc ccaggcgcgc ctgggcgggt gcgccgctga
   108961 gcgcgacctt ccaaccacca agcacctgcc cactttccat gagggcctga cgctgcaggt
   109021 catagccctg ctccaatgac agtgcctgtt ccagctttac cagcggctga gcgtcccgct
   109081 gcgcctggcg cagcgctgca ggcagatcgc ttggcgcgct catgacagct gcctggccag
   109141 tttgccgagt acgtcattca ggcgctcggg cgtcgtgagg gcggccacga agcgatccgg
   109201 gcggaccacg acaatgttgt cgcgcgcctt ggaaaaccat tcggccagac agttatccac
   109261 atcctccacg ctgatcaccc ctgcggtgct tagcggctgg ttgcgtgaac cgctgcgcga
   109321 gcggttgacc tgaataaagc gggtctccag gcccgcccag aatgcctggg tctcctcact
   109381 cagttgttcg agcgggttgc agcggtaggc cagcaccgca taccaggggc ccagcacctg
   109441 atccaggcga atgcgctcgc cgcttgcatc ctccacgtct ggctgaatga atagctgacc
   109501 cactggatcg tcctcacgca catcttggcg ctcgtgatgt accaagcctt tggcaagagt
   109561 agccctaggt ttgaacttga attcagagag gtgcgaacgc aggttatcca ccgtgttcac
   109621 cgcctggaac agccaatccc gcacgccggc gaccagggga ttggtcatcc cgagcatggc
   109681 gcccatgttg tcggccaggg caatcaggtc ggtggcatgg ccacgacgct cctgatcgta
   109741 tgtctggatg attgcgggat tggcccgccc tttgagtacc gctgccaact tccagcacag
   109801 gttggcaacg tcgcgcagcc cggagttgag gccttggccg gcccaaggtg gggagatgtg
   109861 tgcggcatcg ccaatcagcg cgacacggcc agagacgaag cgctcagcca cccgcgagtt
   109921 atgggtgtaa gcgcggatac gcacgatctc gagctgatcg actgcctcgc cgatatgccc
   109981 acggatcatg cggcggatgt tgccctcctc gcacatggcc tcctcgctct cgccttcgag
   110041 caacataaac tcccaacggc gttggctgta cggcaagtaa atgcacacaa agggtcgctg
   110101 cgggtcggcg tgcagcgcgg tgtaaggcgc atccagggtg tcgttggtgg catcaaccac
   110161 cacccatttg cgcgggtggg tcaggccgat cagggaaata cccagttgct tgcgcacgca
   110221 agagcggcca ccgtcggcgc cgatcacata gcgcgcacgc agctcgtacg cctcgccatc
   110281 agcatctcgc acctgcaggg tgacgccgtc gtcgtcctgc tccagcgaaa gcatctcgtg
   110341 gccttcgcgc aggtcaacgc cctcatggcg caccagctcc tcgcgcaagg tgccttcgag
   110401 caactgctgc ataaagatat tgcgcattgg ccagccatag gtggcaacgc ttggcttgac
   110461 ctcggcgaag cacacaccac gcgcgttgta gtaacgcagc ggcacgttgg cgatcatgtc
   110521 gcggtgcgcc cgctcggcca cgccgacggt ctgcagcacg cgtagggcct catcgtccat
   110581 gcccaccgca cgcgggtacg gcaggatgcc ctgagcaagt tccagcacca cgcaatcgat
   110641 gccgtacagg cccatgtaat gagcggcggt gatgccattg gggccgccac caacgattac
   110701 gacctgggta gtttcccgtt tcatcttttg ctcacccgtt tcttattctg atggcttggt
   110761 catgtcccgg aggtatccgg cgacagtacg gttgaacgct tcgccctggt catcgtgtac
   110821 gtaatggctc gcgccagcga tttccacggt acggatgttg ctgttttgtt cctgcatggc
   110881 ggccaacgtc gcaggcggta agaagtccga attgccaccg cgcacgaaca gcgtcggaca
   110941 atccagtgcc cgtacggccg gccaaagatc gaccggttcg atgctcaaac gcgcatcggc
   111001 aatgcccagc tggtcatgcc gccaaacgat accctgcgct gtctcacgca tggagtgggc
   111061 cagacgcaaa cgcaggccct gctccgacaa cccaggacgc gacttaaccc agaatgctcg
   111121 cgccgcttcc cagtcggcaa ataacaacgg cgtttggcgc atctcgcggc ggatgcgctc
   111181 ggcaccatcg ccaacgctcg acgaaccagg accgatgtcc tcgaccacca gcgccagcag
   111241 gcgccccgga ttttgccgtg cgtattccag cgcattggcc ccgccaaggg aatggccgac
   111301 cagggcgaaa tgctgcagct ccaggtgcgc gaccaggtct tccagatcgt gcacgtagga
   111361 ttgggtgtgg tagctgctgc tctcggccca gtcgctcagg ccacgcccgc gctgatccaa
   111421 tgcgtagcag cagaagtcat cgcccagcgc ctgcgccaag gattcccagg tctgcgcata
   111481 ggcacgcaga ccgtgcagca gaatcagcgg cgtgccctgc gggttgcccc agcacaggac
   111541 atgcaggcgc aacccgctgc ggttgttgaa gaactggctg tgcacggtca tcgcaatgct
   111601 cccgatcagg tcaggccgtt atcgcccttg atgtcttctg cgcgcaggcc gcccaagcgt
   111661 gcatgcaaac gcccccgggt ggcgaaggcc cagatgatga tcacttcgtc cggcgccggg
   111721 ccgtcaccga aagtcaccga cacggtgtcg tactgggcgc gcacatagag cgcatccttg
   111781 tgcgccagcg gcacatcgat ggtgcaaccc ggcgcgccac gtttgccggt ggacggcacc
   111841 caggatttgc caccgcccag tgccacgcgg atcgggtccg ctgccgggtt ggtgagaaag
   111901 gcgttaccgt gctcgtactc gccattggcg ccgaccagac aggccttgcc gtagctgaga
   111961 attttccgct cgccggccag ctcacggatg cgccggccga actcctcacc cagtgctggc
   112021 gagtcgtcga tcagtgcgct caggtcctgg ctgaaacgcc cggcataggg attgtgcacg
   112081 gcagcggcaa tcacgtactt gaatagcggc tcgccatcgg ccagttgccc ggactcattg
   112141 gccagggttt cctcggtgaa gctgtaccac ttacggatgt gataattggc gaagttgctc
   112201 gggttcatag cattgtcctc agagggggtc gagagtgatg ttgcgactgg gcgcgcaagc
   112261 ggccttgaag cgggccttag cccggtcgtc gtcgtgatcg gtctcaagga agaattccag
   112321 acggttctcg tccgggtcgt tgaagtacag gccgttgccg atttcgtggt cggtgatctt
   112381 caccacctcc acgcccttgc tcagcaacat gccgtaaagc tgacgcaggg tggtcatgtc
   112441 acccgcgatc tccagcccat agtgctgcag acccaagccg ccctggtgag caccgggcgc
   112501 ggctttgatc agggcgatat cgtggtgctt gctgccgaag gccatcatca cccaggcctc
   112561 accacgggca ctctcgtgca tgccgagtac atcggcatac caaagtgcag aggcattcgg
   112621 gtcggtaacg aacagggaca ggtgggtacg cacgatttcg actttcatgc agagctcctc
   112681 acttcttgct ggcgacagag gtatgggccg gcttgatctg cgcgacggcc ggcgtccaca
   112741 gcacatcggc aatttcactg aaatcgcgcc actgcttgag ccagccgtca ccgccgttcg
   112801 ctgaggtgaa caggtggccg tacttggtac cggcgatgat ctgcttcggg ttgttcgggt
   112861 tgatgccaat gacccagacg caagagttgg gctgttgcgg cagcggcagc acttcccagg
   112921 tctcggccgc gtcgcgcgaa acgaggatct tgctggtggt gccgggcgta ccatcggaga
   112981 tgctcaggta gacggtgtct tcactgccca gcggcgcgtt catggcgcgc acgtagtaca
   113041 ggccaaagtc atccttggcc agttgcccgc tccagctcag gccttcatct tcactgcggt
   113101 aaatggcgtt gacgcacacc accacgatga tcttcttgcc gtgattgggc atgaccagga
   113161 tgttgtggat gtccgagttg tagtcccaca acaggcgttc atcgacacgg gtgaaggtgt
   113221 cgccgccgtc gcggctgtgg aacaggccgc cctcctccag gccgaaccag acctgattgc
   113281 ggtcggtcgg gtcataggcg aaggccagca gccgcggttt gctcacacct tcgcagaatt
   113341 cggggatctc caccggcacg cggtaccagc tctgaccacc atcaagtgtg cgccacagca
   113401 ccgcgcgtga gggtgcgcca gtaccgacga agatgcgctg cgaatcctgc ggatcgacag
   113461 ctactttcca cacggtctgc ccgttgaacg gcgcgtccac cagctcccag tgactgccga
   113521 cgtcgcggct gacaatcaag ccaatatcgg cgccagcgta gatcacttcc ggctgctgcg
   113581 ggtcgaggct cagcgaacgg gtgatggcgt cgaactccag cggctggcca aggccctgac
   113641 ggtgccaggt ggtgccgttg tcagcgctgc ggataatggc ctggccaacc gtcgcgacca
   113701 gaaaggttcc gttgctcata gttgcttgct cctcagatga agatgccgcg agtcaggccg
   113761 ccatcgatgc cgattgattc accggtgatg gagcccgcct tgggcgaggc gagaaaaccg
   113821 atcagccagc ccatttcgat cggctgcaag gtgcggcgga ttggcgtggc gtcgatataa
   113881 ccctgctcaa cctgctgcgg ggtcttgccc tgcttgacgc cctcacgctc gtacagctcc
   113941 tggatgtgcg gggtgtcgac cacgcccggg tggatcagat tgacggtgat acctgccggg
   114001 ccgagctggt cggacagagt cttggtcata tgggcgatgg ccaggttgcg catgccggag
   114061 agcaccttgc tgccacggcc ggtcaggcca ccgatgttga tgatgcgacc gaagcccaac
   114121 tccttcatgt gcggggttac cgccttggaa cagcggaagt agccgatcac cttggtgttc
   114181 aggtccgaca gcagctcgtc gtcaccggca tgttcgatgt cgttgcgcac cacgccagat
   114241 ggagcggcag cgccgttgac cagaatgtcg atgcggccga agtgacgctt ggtctcggcg
   114301 accatctcgg ccaccgcact catctcgttg gtgtcgcaga acagcggcag cacttcgttg
   114361 ccggtctcgg cgctgatctc ttcggcagtc tgcttgaggt attccatgcg ccgggcacag
   114421 atcaccactt tgcagccttc ctgggagaga aaacgcgcca cttccttacc gatgcccatg
   114481 ccgccgccgg tgacgatggc cacgcggtct ttgagttgca gatccatgat caatcctctt
   114541 gttttcgttg ggtcaggcga gatcgacgaa gacgctcttg gtctcggtgt aggcgtcgat
   114601 cacgtgcttg ctcatttccc ggccccagcc ggattgtttg taaccaccga atggcgaggc
   114661 cgggtcgacc acgttccagc agttgatcca caccgaaccg gccttgagct cggcggccag
   114721 gcggtgggcg gcgcgcaggt cacgggtcca caggccagcg gccagcccga agggtgaatc
   114781 gttggcacga ctgaccagct cgtcggtgtc ggaccagctc atcaccgtca ccaccgggcc
   114841 gaagatttcc tcgcgggcca cgcaggcttg ttcggcgcga tcgaggaata cggtcggctc
   114901 aataaagtgg cccttgtcca gatgctcggg acgtttgccg ccacatatca gctcggcacc
   114961 ctctgcctgg ccacgctgca agtagccgct gacgctgttc agttgccgac cggaaaccag
   115021 cggccccatg ctgtttgccg ggttcaggcc ggagccgagc acgtaggccg tggcatgtcg
   115081 gcgcagttcg gcgagcacct gatcaagcac gctctcatgt acgtacaggc gcgaggcggc
   115141 ggtgcacacc tggccttggt tatagaaaat gccgtcggcg gcgcccttgg cggcgcgagc
   115201 aatatccgcg tccgggagga tgatgtttgg cgacttgccg cccaattcca gcgagacctt
   115261 tttcatgttg cttgtggcgg cctgggcgat cagccggccg acctgggtcg agccggtgaa
   115321 ggcaatcttg tccacgtcgg catgctgtgc cagcggcgcg ccggtgcgca cgccaaggcc
   115381 ggtgagcagg ttgaccacgc cagcgggaat gcccgcctgc tcgatcagct gcaccaggcg
   115441 cacagctacc agcggagtct gctcggcggg cttgagcacg gcggtgcagc cggtggccag
   115501 cactgggccg agcttccaca cgcacatggt tagagggaag ttccacggca cgatcagcgc
   115561 acacacaccc acgggctcgc gcagggtgta gttgagcatc ggtgcgccgc tggccggcga
   115621 caccggcaca gtgctgcctt cgatcttggt cggccaaccg gcgaagtaac ggatgatgtt
   115681 ggccgcgctt gccgcctcgc cacgggcgtt ggcgatgggc ttgccgttct ccagggtgat
   115741 cagttgcgcc agctcttcgg cgtacgcctc cagcaggtca gccaggcgca tcatcaacag
   115801 gccacgtcgg gccggcgtct cacgccgcca agggccctcg aaggccgcgc gggccgcggc
   115861 cacagcagcg tcgacgtcgg tttccgaggc ctcggcgact tcgcaaagca cctgctcggt
   115921 ggcagggttc tccacggcaa aggcctgccc gagggcggcg tcctgccagg agccgccgat
   115981 gaacaggcgg cgcggcgtgc tgagaaattc acgtaccgcg ggcaacagct cgatatcgct
   116041 catggtctta cagctccatt tcgatcaggc agggggcagc cgaggcaatg gcacgcgcca
   116101 gggcctgctg cagttcggca ttgctgcgca cgctgctggc cgcgacgccg tagcctttgg
   116161 ccagggcttt ccagtccacg cgtgggcgat ccagctcagt cagggccagg gcctgcgggc
   116221 cgaactcggt gacgccgaag cggcgtagct cgttttgtag gatcgcgtag cggtggttgg
   116281 cagcgatcag gatgactact ggcaattgct cgcgggcgat gctccacaag gtctggatgg
   116341 tgtactgcgc gctgccgtct gattgcaggc agaacacggt attgccccgc tcggccaggg
   116401 ccgcgccaaa gccggccggg atgccctggc cgattgcgcc gccggtgttg gtcagcacgc
   116461 gatggcgcgc cgcccccgcc gaagcggtga agaagggata accacaggtg ccaccttcga
   116521 tagacacgat gcactcatgc ggcagcgctg cggccagcac gcgaccgact gattgcggcg
   116581 tcagcgcctc gtcggccggt ggcaggtcga tgccctgcac actcggcaca tgagccggcg
   116641 cgccgagggc atcggccagg gcgcggagtg caccgaccac gtcgtcaccg acctcggcca
   116701 gcgacagcag gcgatcacgc tcagccaggc gcgaggcgat gccttcgtag ccgaaatagc
   116761 tgatcggctc cggcaccccg gcacacacca ccaggtcgaa ctgatcaagg atttcgatgg
   116821 ccacttccgg gaagtacggc aggcgatcca gatccggcag gccaccacct cgatggctga
   116881 cccgcgggaa ggtctcggcg aacaggcgca cgccgctgag ctgcgccagg cgaccggccg
   116941 cttccaggcc agcagccgtc aggccgttat caccgacaat aaacaccagg cgcttaccgg
   117001 ccttgagttc agccgccaca cgctcgacct tatcgccagc aaagcggcgg atcggcggtt
   117061 gcatcggggc gaagcgcagc gcctcggccg tgaccgcgct ggcttgtagg tccattggca
   117121 ggatcaggct ggcgatctgc ccgcgcggct gccaggcggc ctgcacggcg cactgcaggt
   117181 cctcaccgat gcgctgggca tggcgcgaag tgcgcaccca accggacaca gtgccggcca
   117241 gggcttcgat atcgctggcc aggggcggat cgtagttgcc gtgccaggag gcgtgatcac
   117301 cgatcacgtt gacgatcggc gtattggcac ggcgggcgtt gtgcaggttg gcgatgccat
   117361 tggcgaagcc tgggccaagg tgggtcaggg tcatggcagg ctttccggcg atgcgcccat
   117421 aaccatcggc ggcgcccgta cagacgccct cgaacatcga cagcaccggc ttcagcgcag
   117481 gctcagtgcc cattgcggca accagcggta tctcagtggt tccggggttg gcgaagcagt
   117541 actcgacacc gctggcaaca gcggccttga taagcaattc ggcaccattc atttggcaat
   117601 cccatggcat gcgttgacga ttgcgagatt tattgcatcg cactggaatc acaacaagta
   117661 ttttttaaca aaaggctcaa aatacgagat aaatgagatt tttaaaccta aaaacatctc
   117721 gaaaacagcg ctcaatccca gctatgcttt ctcgcagtcg tccaccggcg ccttctacgc
   117781 agggcgtaat ctgaaaggaa taaaatgagc agcttgagca agaccctggg ggtgctggac
   117841 ctgttcggcc cgcagcaact acaactggac ccggaaacca tcgccgaacg catgggactg
   117901 tcgcgcgcca cggtctaccg ctacgtcaaa gacctgtgtg aggccggact actgagccgc
   117961 gtcggtgccg gcaactacgc cctcggccca cgcatcatcg agctggactg gatgatgcgg
   118021 cagtacgacc cgatcctcat tgcgggtcgc gagccgatgc agcgcttgtc tgaggagacc
   118081 ggcctggcgg tgttcgccag cgttttctat gacggccaca tcatcaacac ctacatcacc
   118141 gaccccaacg agcagcacca tttcgcgttc ggccgtggtc atccgctgcc accgtttcgc
   118201 ggtgcacagt cgaaggtgct ggtggcccac cagaagggtc ggcgcctgct gaaactgttc
   118261 gaggaacaga tttccaagga ccccaataat ccctacagca cgcgcagctg gtcggagttc
   118321 gctcgggcgg cgaagaagat ccgcaaggac ggctactgca tcacccatga cgaactgaac
   118381 ctgggactca ccggtattgc cgcgccgatc atcgtcatgg acggcaagga ggtggtcggc
   118441 agcgtctcgg cggtcggcac caccgatacc ttcaagctgc tgcgccagga gcgcgttatt
   118501 gacctggtga tccagaccgc caagctcatc gcgaccaaca tgcaaaagcc gcagagcaca
   118561 tcaggccctt cgtaaaccaa ccaatttacc gatataaaat ctcataaatc tcttatattg
   118621 agcatttttc taaaaatgat ttgataataa tcacgaaaat ataaaaaaat ctccgaacaa
   118681 atcgccaccg gcaccttgga ggcgcacacc atgtccgccc tttccatgct accggcggtc
   118741 atgtttcttt cggagccatg cgatgaaact caaccacacc aacttgctac gcgaacaggc
   118801 ctatatcgac ggccagtggg tcgacgccga caaccaagcg ctcttttccg taaccaaccc
   118861 ggccaacggc gaattgatcg ccatggtcgc ctcggtcgga caggccgaaa ccgcccgcgc
   118921 catagccgcc gccaaggccg ccctgcccgc ctggcgcgag aagaccgcca aagagcgcag
   118981 cgccatcctg cgcagctggt acaacctgat catggaaaat caggaagacc tggcccgcct
   119041 gctcagttgg gaacagggca aacccctggc cgagagccgc ggcgaaatcg cctacggtgc
   119101 cagctttatc gaatggttcg ccgaggaagc caaacgcatc tacggcgatg tgatccccca
   119161 cgacaagcaa ggccgtcgcc tggtggtgat caagcaggcc atcggcgtgg tcgcggcgat
   119221 caccccgtgg aactttccca acgccatgat cacccgcaag gtcggcccgg ccctggccgc
   119281 cggctgcacc gtggtggtca aaccggcctc ggaaacccca ttgtcggccc tggccctggc
   119341 ggtgctgggc gagcgcgccg ggattcccgc tggcgtgctg aatatcgtgc ccggcaccga
   119401 ctcgcgcgcc atcggcggcg agctgaccgg caacccggac gtgcagaagc tctcgttcac
   119461 cggctccacc ggtattggca aactgctgat ggcgcagtgc gcggcgacca tcaagaaggt
   119521 ctcgctggag ctgggcggca acgcgccctt tatcgtcttc gacgacgccg acctcgacgc
   119581 cgccgtgcaa ggcgcgctgg gctcgaagtt ccgcaacagc gggcagacct gtgtgtgcac
   119641 caaccgcttt ctggtgcaga acggcgtgta cgaggaattc agccagcgcc tggtcgccgc
   119701 ggtaaacgcc ctcaaggtcg ccccggccga tgaagacggt gctcaacagg gcccgttgat
   119761 caacgctaat gcagtggcca aagttgagga gcatatcgcc gatgcggtca gcaaaggtgc
   119821 caaggtactc gccggcggcc aaccgcatgc
//
LOCUS       DASRUM010000252        20938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_465596, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000252 DASRUM010000000
VERSION     DASRUM010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 20938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20938
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465596"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1381
                     /locus_tag="VFF22_16985"
     CDS             50..1381
                     /locus_tag="VFF22_16985"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZX18549.1"
                     /translation="MIRKHSFRGVMAGFATSALALAVSAQAFAGTVSTDGADLVIKTK
                     GGLEVATTDKEFSFKLGGKLQWDATNFDGLQAADQNNPFDDTFNTFIRRGEIGLEGVA
                     YKDWGWGVRLSYDGLAEGTDVDRAYITYTGLDLATFTVGRFGVDYGLEGTTSSSWITA
                     IERPFMYDFLHGDEDAQFGVNVKHSGDNYGLMAQVATYDESDNANNYESDDNDELFGY
                     TLRAHWAPYLNGTDVVHLAANYHDSSSDDDRARARTRMGIRSDNDFRLTFGDVRVADQ
                     DSEWLLESGVQFGGLRGQAEYFQRSITGENLVGADSDVDMSGYTAQVSYMFGAARGYK
                     GSDGKWDKPTDMKGTWEVFARYESTEIDSDATAIPGNLGLAGVVAADAGDEFEASAVV
                     VGVNYFATPAVRMSLNYVDYQVDNIDTNSQVGGENVQDDGKAIIGRLQYVF"
     gene            1703..2827
                     /locus_tag="VFF22_16990"
     CDS             1703..2827
                     /locus_tag="VFF22_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX18550.1"
                     /translation="MLLSQLNSINEIAASQWDALLTDAQPFLRHAFLSALEDSGSVGG
                     RSGWQPAHAVLKGEQGQLLAAMPSYVKSHSYGEYVFDHGWADACHRAGIEYYPKLLGA
                     IPFSPVTGQRLLGAPQAAGQLLDGLSAGLEQQGLSSLHINFTTEAENALLQGRDGWLQ
                     RLGCQFHWRNRGYRDFQDFLDALSSRKRKQMRKEREQVAGQGIEFDWREGHQLSELEW
                     DFVYLCYANTYRVRGQSPYLTRAFFSLLAERMPEMIRVVLAHQAGRPVAMAFSLVGGD
                     SLYGRYWGCLAEFDRLHFETCFYQGMDHAIAAGLQRFDAGAQGEHKLIRGFEPVITHS
                     WHYLCHPGLRNAVANFLVEEQHGVRGYAEDARGLLPYRQD"
     gene            2989..3468
                     /locus_tag="VFF22_16995"
     CDS             2989..3468
                     /locus_tag="VFF22_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZX18551.1"
                     /translation="MDERYSYKKAPGHAALRRGRVSLSNTVYLITATSENREPLFAHF
                     PTACAAARCFEDPALLRSSRMLAWVLMPDHAHWLLQLGDADRLGDLVGRLKSASSRHA
                     NQSLNRTGSLWSKAFHDHVLRNEQDLQAVARYIVANPLRAGLVKRLGDYPFWNAIWF"
     gene            complement(3556..5388)
                     /locus_tag="VFF22_17000"
     CDS             complement(3556..5388)
                     /locus_tag="VFF22_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010793237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX18552.1"
                     /translation="MLFRRFEHLIDVFKASPDVEPPAGMLAFYAYYLRQVWPLMIAVL
                     VVGFVAALIEVALFSFLGQLIDMTQNTPSGEFFEQHQGTLLWMLLVALVIRPLVFGLH
                     NLLTHQTINPGLTNLIRWQNHRYVLKQSLSFFQNDFAGRIAQRIMQTGPSLRDSAMQV
                     VDALWHVLVYAVSALYLFAAADLRLITPLLLWIVGYCAALWYFVPRIKERSAADSAAR
                     SKVMGRVVDGYSNVTTLKLFAHTQVEEHYAREAMQELLGKVRLQSRIITTLDFLITCL
                     NGMLIVGTGALALWLWDQQLISTGAIALALGLVIRINGMAEWIMWVVNGIFENVGTVQ
                     DGMKTIVQPRQVLDQAQAKPLQVSQGAVQFDNIHFHYGKRNGVIAGLSLTVAPGEKIG
                     LIGPSGAGKSTLANLLLRLYDLESGRILIDGQNIAEVAQESLRAQIGMVTQDTSLLHR
                     SIRDNLLYGKPEASEAQLLDAVRKARADSFIPLLDDGMGGHGFDAQIGERGVKLSGGQ
                     RQRIAIARVLLKDAPILVLDEATSALDSEIEAAIQESLDTLMQGKTVIAIAHRLSTIA
                     RMDRLVVIDQGRIIETGSHSELIAAGGLYARLWQHQTGGFVGVE"
     gene            complement(5514..6068)
                     /locus_tag="VFF22_17005"
     CDS             complement(5514..6068)
                     /locus_tag="VFF22_17005"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZX18553.1"
                     /translation="MFKTLTLAACSILFAGSLLAAENPKVLLTTSLGDIEIELDAEKA
                     PISTANFLAYVDSGYYAGTQFHRVIPGFMVQGGGFDADMQQKDTQAPIKNEADNGLHN
                     VRGTLAMARTQVRDSATSQFFINHKDNAFLDHGSRDFGYAVFGKVVKGMDVVDKIAQV
                     PTANRGGHQNVPREPVLITAAKRL"
     gene            complement(6081..6893)
                     /locus_tag="VFF22_17010"
     CDS             complement(6081..6893)
                     /locus_tag="VFF22_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX18554.1"
                     /translation="MSFFDNDDCQLHYEEYGHGAPVLLVHGLGSSTRDWEYQIPELAR
                     HYQVIALDVRGHGRSDKPRERYSIQGFAEDVAALIEHLQLPQVHLVGISMGGMIGFQL
                     GVDRPELLKSLCIVNSGPEVKAKSPRDYIEIAKRWTLSRLLSLDTIAKALGRLLFPKP
                     EQEELRQKILARWPQNDKRAYLASLDAIIGWGVRERLARISCPTLVITADRDYTPVAQ
                     KQAYVDEMANARLLVIDDSRHATPLDQPEQFNSSLLAFLAEVEQAAPTAKPQ"
     gene            complement(6907..7833)
                     /locus_tag="VFF22_17015"
     CDS             complement(6907..7833)
                     /locus_tag="VFF22_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX18555.1"
                     /translation="MKAPRVTLDQWRTLQAVVDHGGFAQAAEVLHRSQSSVSYTVARM
                     QEQLGVPLLRIDGRKAVLTEAGDVLLRRSRQLVKQASQLEDLAHHMEQGWEAEVRLVV
                     DAAYPNARLVRALTAFMPQSRGCRVRLREEVLSGVEEVLKDGMADLAISALNIPGFLG
                     AEMSTVEFVAVAHPDHPLHRLQRVVTFQDLETQMQVVIRDSGRQQPRDVGWLGAEQRW
                     TVGSLATAATFVSNGLGFAWLPRHLIERELKDGQLKPLPLDQGGSRNPLFFLYSNKDK
                     PLGPATQILVELIQRFDAAPLNAAFAAPLPSA"
     gene            7921..8235
                     /locus_tag="VFF22_17020"
     CDS             7921..8235
                     /locus_tag="VFF22_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate kinase"
                     /protein_id="HZX18556.1"
                     /translation="MKKLCCALLACLPLTVMAYPIEVEKQLNGAEVSATTQEIDHNMG
                     GLQLYNYGRSDAKCAAVFRNGPEAPRTRKVSLAAGESSNLAVKFSRSIIKLRVKLTCE
                     LS"
     gene            8393..8992
                     /locus_tag="VFF22_17025"
     CDS             8393..8992
                     /locus_tag="VFF22_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008018225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HZX18557.1"
                     /translation="MANVLVIESSARQQGSISRQLTAQFIANWSAANPADQIKVRDLA
                     VDQVPHLDANLLGGWMTPAGQQSEAEQAALALSNLLTDELLAADVLVLAAPMYNFAIP
                     STLKAWLDHVLRAGVTFKYSETGPQGLLSGKRAFVLTARGGIYAGGTQDHQEPYLRQA
                     LAFVGIHDVSFIHAEGLNLGGDFMEKGLNQAKAQLAQAI"
     gene            9191..10087
                     /gene="rarD"
                     /locus_tag="VFF22_17030"
     CDS             9191..10087
                     /gene="rarD"
                     /locus_tag="VFF22_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HZX18558.1"
                     /translation="MQLSGRGVALSIIASVLFAVVPGYVRLLAPLDGLQVFAQRVLWS
                     MPAVLLLITLSRQWPVLLAACGRVRREPLLLASLPLAALLMGIQWALFVWAPLAGRML
                     EVSLGYFLLPLAMVLAGRVFYGERLRPLQRLAVFCALLGVAHELWLTQAFSWVVMVTV
                     LGYPPYFMLRRWMRLDALSGFAFEMLFMAPVAIWLVLQWSPPQLFSQAPQLWWLLPGL
                     AILSSLAFAAMMAASRLLPMGLFGILSYVEPVLLFLVALLFLGEQFNSAQWLTYLPIW
                     LAVLLVGWDSARLLLKQARQGL"
     gene            complement(10082..10636)
                     /locus_tag="VFF22_17035"
     CDS             complement(10082..10636)
                     /locus_tag="VFF22_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZX18559.1"
                     /translation="MKPTLIYYVAASLDGYIARPDGRVDWLEAIQASGDDHGYHAFYD
                     GIDALLMGRMTFNTLMASEGSWPYPGKPCRVLTRYPHEQLPEGVSMHHCTPIEALTEL
                     AEQGHQRVWLVGGGSLAGNCFAAGLLDELVVSLVPYLLGAGIPMFSSGLERSLRLHEQ
                     RSFSSGVVQLHYQVLPENPAGIDL"
     gene            complement(10733..11386)
                     /locus_tag="VFF22_17040"
     CDS             complement(10733..11386)
                     /locus_tag="VFF22_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HZX18560.1"
                     /translation="MLITAPQRLRNALMALVLFHILIIIASNYLVQLPITLFGWHTTW
                     GAFSFPFIFLATDLTVRLLGKRTARLVIAQVMLPALLISYIVSVLFQEASFRGFGALL
                     EFNEFVLRISIASFLAYVLGQILDIQVFDRLRQLSLWWVAPTVSTIFGNLLDTYAFFS
                     VAFWNSSNPFMAEHWVEIATVDYGVKLAVSLLLFVPLYGMLLNAILRILAGRPQTTS"
     gene            11640..12989
                     /locus_tag="VFF22_17045"
     CDS             11640..12989
                     /locus_tag="VFF22_17045"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZX18561.1"
                     /translation="MFSQFALHERLLKAVAELKFVEPTPVQLAAIPLALQGKDLRVTA
                     QTGSGKTAAFVLPMLNRLLGDGASQPRMSVRAMILLPTRELAQQTLKEVERFAQFTFL
                     KGGLITGGEDFKVQAAMLRKVDILIGTPGRLIEHANAGNLLLDEVEVLVLDEADRMLD
                     MGFAEDVQRLSELCNADRQTLLFSATSGGAVLREVVGKVRKEPEHLQLNRVSQLNEGT
                     RQQIITADHNHHKERLVEWLLKNETYDKAIIFTNTRVQADRLYGKLVAHDFKTFVLHG
                     EKDQKDRKLAIDRLKQGAVKILVATDVAARGLDVDGLDLVINFDMPRSGDEYVHRIGR
                     TGRAGAEGLAISLICHNDWALMSSIERYLKQRFERRNIKDLKGIYQGPKNLKASGKAV
                     GPKKKKIDPKTGKKLATKKPAAKAPKRNTINRPKPDAPVLVSQDGLAPLKRRAPAAE"
     gene            12994..13515
                     /locus_tag="VFF22_17050"
     CDS             12994..13515
                     /locus_tag="VFF22_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003158871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HZX18562.1"
                     /translation="MVWRLGLFAALAVAAQAQGAVHKCVQPDGVVLYTDQPCSIAHSN
                     LALSVSDYASYPPLLQGVAQLWHERVQPLLVGVDVLALLALVYGLMSLVCYIAYYRDK
                     QFAIKGQQRTPEARLHLYELLGGWPGGLLAQRLIRHKNRKLSYQLLFWLIVLLHLSLA
                     GLVLWLINTPAGG"
     gene            complement(13551..14531)
                     /locus_tag="VFF22_17055"
     CDS             complement(13551..14531)
                     /locus_tag="VFF22_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HZX18563.1"
                     /translation="MLKAEYQTRGPVPQEAIAAVALQLAEPGAGQVRIKVLAAPINPS
                     DVLTLTGEYGMLPPLPAIGGNEGVGRVEALGAEVSNLKVGQMVLLPVGCGTWVSHLNA
                     PANKLIPLPEADPQQLAMLTVNPPTASLMLSEFVDLKPGDWVIQNAANSGVGSYLIQL
                     AKLRGFKTINVVRRESAVAGVEAEGGDVVLVDGPDLAKRVRAATGGADVRLGIDAVGG
                     ESTDHLAASLCDGGVLVNYGMMSRQPCQVSPASFVFRDVSLRGFWLAKWFQNASQAEQ
                     MKVFGELVQLIASGKLHTRVAATYDVSEIKQAVAAAASGERDGKILIVPK"
     gene            complement(14653..14958)
                     /locus_tag="VFF22_17060"
     CDS             complement(14653..14958)
                     /locus_tag="VFF22_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18564.1"
                     /translation="MNTNLPSKTAIGLCAVFALFAQSPTYASCTTEEATAKAGELAAK
                     VAEITKKDPARAAQLREELKETSPETSADELENACEGYDQRLLELEEAGEEVEDSQN"
     gene            complement(15051..16817)
                     /locus_tag="VFF22_17065"
     CDS             complement(15051..16817)
                     /locus_tag="VFF22_17065"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012076628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HZX18565.1"
                     /translation="MRLSQPLLAITLLIITAAVQAQAPLRLVHLQRCGDLFDKHQQQF
                     CLESQGLQRGAFKLMFDGAPLPSAAVERSGKKIQVTIAQGRQQSGPIWLEQQGQASNP
                     VWLSLKGSHVVAANPDEVAKNMDGLTTYVDLISLIIEEDADGLAVSRQLAQKYGATVV
                     GTIAPLNTYQLRLPVDNLIERDAMVLRLGSEVSVDAVVIEESGAEEGDAVTQDNPAAN
                     TPHQNEWAANRFLDAVNYYQRRVPAKNSPIPTTPVRIGLIERDVDFDAADFNDYLGAA
                     ERADGKPHTRLYARDAARPDGHGSTVAGILAARWDKGGNTGFLRGLDGAGPGFEVIVE
                     RNSDAGITANIAASVNLVEDGVRVLNWSWGLHRIGAKNIRGDEVDSLVRSGLAMSGYE
                     ELLEEFFLWLRSEHPEVVVVNSAGNGASFSGADEYRLPSSFITDQLLVVGGHQRSDRD
                     VPVTDADYAIKRSSSNSDTRVDISAAACVQASTIGANEQGDIHCGTSYATPLVAGLVA
                     GLLSINPDLHPAQLRMLLRRSAMTLGEEFDFEPTDAEDLTAPILPSERNYELDNPDIG
                     RSARLDMQKALDLAVKSRDRVR"
     gene            17020..17412
                     /locus_tag="VFF22_17070"
     CDS             17020..17412
                     /locus_tag="VFF22_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZX18566.1"
                     /translation="MSYNWDLIERLLHEVQNSAGKNFAPRAYAEELAQELERAGEHTS
                     NLDALKAEATEYESQLLSAGFIEPRPEEEGGNGENFVLTPRGLQLLSMIDSSIPGGEH
                     SRELLDDQGTAALVPEVFDETAGKANLV"
     gene            17592..18314
                     /locus_tag="VFF22_17075"
     CDS             17592..18314
                     /locus_tag="VFF22_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX18567.1"
                     /translation="MKWLWVCCLLSCSLTVSAQGLRVGFGTHKPPYIFENEARGLEHD
                     IVMAAAQRGGLEPVAYYAPMERLNLMLSKGQIDVIATTNERSGGSIFYSDAYIRYQNV
                     AVALRSRNLDIQRISDLVGYSVNAFQRARFLLGSEYQAMAENNPRYREEAFQIARNRM
                     LYSGRVDVVVTDMRILRYFNREVYTQVDVTQPLALYPIFAATDYKLGCRQQAHCERFN
                     QGLAEIRESGEYRLIERRYAMH"
     gene            complement(18333..20558)
                     /locus_tag="VFF22_17080"
     CDS             complement(18333..20558)
                     /locus_tag="VFF22_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HZX18568.1"
                     /translation="MRVTCNWLRHPSSTLLTLGLLLLALPLLSRYTLGWSHAYGYLSD
                     LAIGSLLLVVLHQRSWLLSIPLLLAWCIFTLSTAELVNAVGRMPEPADLQYLGDAQFV
                     SHSTQGSGLSQPLLALAMAVLAALYLGLRSVQTPRSAMPLARTWLLLPLALFAVHSVG
                     QQFKPSEADQWLQFNLPHKLLAESSNSAQQHLSDWLAGDEPSSPPDISGLNQLDLSGT
                     PLLKQAGSARNVLIITLEGVTGAYLQASRQAIGSSYTQDPMPRLSQWAERGMLTTDYV
                     LHGHQTIRGLYAMLCGDYNKLDSGTPKGVELLNNPARSEQCLPAQLRERGLSTHFLQG
                     AGLRFMAKDQIMPQMGFDKTLGRDWFKNKPYLEFPWGMDDKAYFEGALGYVKQLRKKR
                     QPWMLTLLTVGTHQPYSAPQDYLDRHPNAKLAAIAYLDDAVADFLNALEKQGVMKDTL
                     VIVTSDESHGLENVRLASTWGFNLLLAPEQAQLPALKQGVYGHVDLTASVLDYFAFPV
                     PANIAGRSLLRDYATGREIISYTNGLLRRHDGQGTLTECDFQNVCRRYASPGFIADSA
                     RYLGRFSGKPARLISQQAALLDQSLSNGGQAEQRYQFAHQQPIKLKPSVSDDWADNLV
                     GAQYLTLGAATQTTVTLKIRALRMDKHGATLQLKTKEYDRNVDLAIPELPLLTRNKPV
                     EVSFTFDNPQARKAFSFHLLGQGQGSIEIIDFSVSSTPLQTEEELLAAQEGLDEVAPT
                     P"
     gene            complement(20788..>20938)
                     /locus_tag="VFF22_17085"
     CDS             complement(20788..>20938)
                     /locus_tag="VFF22_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008719741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18569.1"
                     /translation="KPAPAVETSASIDAQIEAFLKSGGEIQQIAKGVSGQVYGTTKQI
                     TIGKK"
BASE COUNT         3872 a         6087 c         6661 g         4318 t
ORIGIN      
        1 agcgctcggg tgttttcaac acttaaaagt ccatttagag gaatcttcga tgatccgtaa
       61 gcacagcttt cgtggagtaa tggccggctt cgcaaccagc gctctggccc tggccgtttc
      121 cgcccaggct ttcgccggta ctgtcagcac tgacggcgcc gatctcgtga tcaagaccaa
      181 gggtggcctg gaagtcgcca ctaccgacaa agaattcagc ttcaaactgg gcggtaagct
      241 gcagtgggat gccaccaact ttgatggcct gcaggcagcc gatcagaaca atccgttcga
      301 tgacaccttc aataccttta tccgccgcgg tgaaatcggc ctggaaggtg tggcctacaa
      361 ggattggggc tggggcgtgc gtctgtccta tgacggcctt gcagagggca ccgatgtcga
      421 tcgtgcctat atcacttaca ccggtctgga tctggctacc ttcactgtcg gccgcttcgg
      481 tgttgactac ggtttggaag gcaccaccag ctcgtcgtgg atcaccgcca tcgaacgtcc
      541 atttatgtac gacttcctcc acggtgatga agacgctcag tttggcgtaa acgttaagca
      601 ctctggcgac aactacggcc tcatggctca ggttgccacc tacgatgaaa gtgacaacgc
      661 caacaactac gagtctgatg acaacgacga actgttcggt tacaccctgc gtgctcactg
      721 ggctccgtac ctgaatggca ctgatgttgt tcacctggct gccaactacc acgacagcag
      781 ctcggacgat gaccgtgctc gtgcccgtac ccgtatgggt atccgcagcg acaacgattt
      841 ccgcctgacc ttcggtgatg tgcgcgtggc tgaccaagac agcgaatggc tgttggagtc
      901 gggtgttcag ttcggtggcc tgcgtggcca ggctgaatac ttccagcgca gcattaccgg
      961 tgagaacctt gttggtgctg attccgatgt ggatatgagc ggttacaccg cgcaggtttc
     1021 ctacatgttc ggcgcggctc gtggttacaa aggcagcgac ggcaagtggg acaagccgac
     1081 cgatatgaaa ggcacctggg aagtcttcgc tcgctacgaa agcaccgaga ttgactctga
     1141 tgcgaccgcc attccaggca acctgggcct ggccggtgtt gtggctgcgg atgccggtga
     1201 cgagttcgaa gccagcgccg tggtggttgg tgtgaactac ttcgccactc ccgctgtacg
     1261 catgagcctg aactacgttg actaccaagt agataacatc gacaccaact cgcaagtagg
     1321 tggtgaaaac gtgcaggacg acggcaaagc catcatcggc cgtctgcagt acgtgttcta
     1381 agtaataccg ctcaactgct gctcctttct ttgaaccccg cctagtgcgg ggtttttttt
     1441 cgagttttta tgggcagaag gactgatgcg cgtctgtgct ctgctgttac tggtaatggg
     1501 gttgagcgct gtgaccgtag ctcgccaaac cgggtgattg gttgtcggtg cgctttgagc
     1561 cgttctactc aatgcaggcg gtggtggctt gcttgcttgc cttgccttgc ctggattttc
     1621 aatgatctgc cagtaaagcc atcgcagcgc ccaggcatgc ggcaatatat agtgcagtct
     1681 tgcctgactc gccgagccgt cgatgttgct gagccaactg aattccatca atgaaattgc
     1741 cgccagtcag tgggatgctc tgctgactga tgcgcagcct ttcttgcgcc acgcctttct
     1801 ttctgcactg gaagacagcg gcagcgttgg tgggcgcagt ggttggcaac ctgcccatgc
     1861 ggttttaaag ggcgagcagg gccaactgct ggccgcgatg ccgagctatg tgaaaagtca
     1921 ctcctatggc gaatacgtgt tcgaccatgg ctgggcggat gcctgccacc gggccggtat
     1981 cgagtattac cccaagctgc tcggcgcgat tcccttctcc ccggttaccg gtcagcgctt
     2041 gctgggcgca ccgcaggcgg ccgggcaact tctcgatggc ttgagcgccg gcttggagca
     2101 acagggcctg tcgagcctgc atatcaactt caccactgag gctgagaatg ccctgctgca
     2161 gggccgcgac ggctggttgc agcgtctggg gtgtcagttt cattggcgca accgcggtta
     2221 cagggacttt caggattttc tcgatgcgct cagctcgcgc aagcgcaaac agatgcgcaa
     2281 ggagcgcgag caagtggcgg ggcagggcat cgaatttgac tggcgtgagg gccatcagct
     2341 cagtgagctg gaatgggatt ttgtctacct gtgttacgcc aacacctacc gggtgcgtgg
     2401 ccagtcaccc tacctgacgc gcgccttctt cagcctgttg gccgagcgta tgcccgagat
     2461 gatccgcgtg gtgctcgctc atcaggccgg tagaccagtg gctatggcct ttagcctggt
     2521 cggcggcgac agcctgtacg gacgctactg gggttgcctg gccgagttcg accggctgca
     2581 tttcgagacc tgtttctacc agggtatgga ccatgccatc gctgctggcc tgcaacgttt
     2641 cgacgccggt gctcaaggcg agcacaagct gatccgcggc tttgagccgg tgatcaccca
     2701 ctcctggcac tacctctgcc accctggcct gcgcaatgcc gtggcgaact tcctcgtaga
     2761 ggaacagcac ggcgtgcgcg gttatgctga ggatgcacgc ggtttgttgc cgtatcggca
     2821 ggactgagag ttttcgcggg catggagcta agcgtcccct tgcccttcga tcagggctca
     2881 cacgcacgta tctgaaaccg cttcgcgggc atggcccgct cctacaggat gaccaccgca
     2941 ctactagtag gagcggccat gcccgcgaat ttctcaagga gaacgcttat ggatgagcgt
     3001 tacagctaca aaaaagcgcc aggacacgcg gccttgcgta gaggtcgtgt ttccctgagc
     3061 aatacggtct atttgataac tgcaactagc gaaaaccgcg agccgctgtt cgcccatttc
     3121 cctacggcct gcgcagccgc acgctgtttt gaagaccccg ccttactcag aagtagccgc
     3181 atgctggcct gggtgttgat gccggatcat gcgcactggt tactgcagct tggcgatgcc
     3241 gaccgcctcg gcgatctggt gggtcggctt aaatccgcca gttcccgcca tgctaatcag
     3301 agtctgaaca ggaccggttc gctgtggtcg aaagcctttc atgatcatgt cctgcgtaat
     3361 gagcaagacc tgcaagcagt cgcccgttat atcgtggcca atccgttacg agcggggttg
     3421 gttaagcggc tcggggatta tccgttctgg aatgccatct ggttttgata cggccccgca
     3481 ttggtggtgt atctacatct tttcgcggcc atgggccgct cctacggggg gagtggtgta
     3541 tccgcgttct gcgttttact ccactccgac aaatccgccg gtctggtgct gccaaagccg
     3601 cgcgtagagg ccgcctgcgg caatcagctc actgtggctg ccggtttcga taatgcgccc
     3661 ctgatcgatc accaccaagc ggtccatgcg cgcgatggtc gacaggcggt gggcgatggc
     3721 gatcaccgtt ttgccctgca tcagggtatc caggctttcc tggatcgccg cttcgatttc
     3781 cgagtccaac gccgaggtgg cttcgtccag caccaggatc ggtgcgtcct tgagcagcac
     3841 gcgggcgatg gcgatgcgtt ggcgctggcc accggaaagt tttaccccgc gctcgccgat
     3901 ctgcgcgtcg aacccatggc cgcccatgcc gtcgtccagt agcgggataa agctgtcggc
     3961 gcgggccttg cgtacggcgt cgagcagctg ggcttcgctg gcttcgggtt tgccgtagag
     4021 caaattgtcg cggatcgagc ggtgcagcag cgaggtgtcc tgggtgacca tgccgatctg
     4081 cgcgcgcaga ctttcctggg ctacctcagc gatgttctgg ccgtcgatca ggatgcgccc
     4141 gctctccagg tcgtagaggc gcagcagcag gttggccagg gttgatttgc cggcgccaga
     4201 agggccgatc aggccgatct tctcgccggg cgccactgtc aggctcaggc cggcgatcac
     4261 gccgttgcgt ttgccgtagt gaaagtgaat gttgtcgaac tgcacggcgc cctggctgac
     4321 ttgcagcggt ttggcctgcg cctggtcgag cacctggcgc ggctggacga tggttttcat
     4381 gccgtcctgc acggtgccga cgttctcgaa aatgccgttg accacccaca tgatccattc
     4441 ggccatgccg ttgatgcgaa tcaccaggcc cagcgccagg gcgatagcac cggtgctgat
     4501 cagttgctgg tcccacagcc acagggccaa cgcgccggtg ccgacgatca gcatgccgtt
     4561 aaggcaggtg atcaggaaat ccagggtggt gatgatgcgc gactgcaaac gcaccttgcc
     4621 gagcaattcc tgcatggctt cgcgggcgta gtgttcctcg acctgggtgt gggcgaacag
     4681 cttgagggtg gtgacgttgc tgtagccgtc gaccacccgg cccatgactt tcgagcgcgc
     4741 cgccgagtcc gccgcagagc gttccttgat gcgcggcacg aaataccaga gcgccgcgca
     4801 gtaaccgact atccacagca gcagcggtgt gatcaggcgc aggtcggcgg cggcaaacag
     4861 gtacagcgcg ctgacggcat acaccagtac atgccagagc gcgtcgacca cctgcatcgc
     4921 tgagtcgcgc agcgaggggc cggtttgcat gatgcgttgg gcgatgcgcc cggcgaagtc
     4981 gttctggaaa aagcttaggc tctgcttgag cacataacgg tggttctgcc agcggatcag
     5041 gttggtcagg ccagggttga tggtctgatg cgtcagcaga ttgtgcaggc cgaacaccag
     5101 cgggcggatg accagcgcca ccagcagcat ccacagcagg gtgccttgat gttgctcgaa
     5161 gaactcgcca cttggggtgt tctgcgtcat gtcgatcagc tgaccgagga agctgaacag
     5221 cgccacttcg atcagggcgg cgacaaagcc gaccaccagc acggcgatca tcagcggcca
     5281 gacctggcgc aggtagtagg cataaaacgc cagcatgccg gccggcggct cgacatccgg
     5341 gctggctttg aagacgtcaa taaggtgttc gaagcggcga aacaacatgt caggcatcct
     5401 gcctagatca gggaatagcg cccgattcta acgagtttgc cggggagggt gttgcgcaga
     5461 aaaaaggagg gttaaagcgc acggggtgag caggcccacc ccgcgtggag ttatcagaga
     5521 cgcttggcgg cggtgatcag caccggctca cgcggcacat tctgatggcc gccacggttg
     5581 gcagtcggca cctgggcgat cttgtcgacc acgtccatgc ccttcaccac cttgccgaac
     5641 accgcgtaac cgaagtcgcg cgagccgtgg tcgaggaagg cgttgtcctt gtggttgata
     5701 aagaactggc tggtggccga atcgcgtacc tgggtacgcg ccatggccag ggtgccgcgc
     5761 acgttgtgca ggccgttgtc ggcttcgttc ttgatcggtg cctgggtgtc tttttgctgc
     5821 atgtcggcat cgaagccgcc gccttgcacc ataaagcctg gaattacccg gtggaactgg
     5881 gtgccggcgt agtagccgct gtcgacgtag gcgaggaagt tggcggtgct gatcggtgcc
     5941 ttctcggcat ccagttcgat ctcgatgtcc ccgaggctgg tggtcagcag cactttcggg
     6001 ttctcggcgg ccagcaggct gccggcaaac aggatggagc aggcggcgag ggtgagggtc
     6061 ttgaacatgc gtggtagtcc ttattgtggc ttggcagtgg gagcggcctg ttcgacttcg
     6121 gcaaggaagg ccagcaggct gctgttgaat tgttcgggtt ggtccagcgg tgtggcgtgg
     6181 cgcgaatcgt cgatcaccag caggcgcgca ttggccattt catcaacgta ggcctgcttc
     6241 tgcgccaccg gggtgtaatc gcggtcggcc gtgatcacca gggtcggaca gctgatccgc
     6301 gccaggcgtt cccgcacgcc ccaaccgata atcgcatcca ggctggcaag gtaagcacgc
     6361 ttgtcgttct gcggccagcg cgcgaggatt ttctgccgca gctcttcctg ctccggcttg
     6421 gggaacagca gtctgcccag ggctttggca atggtgtcga ggctgagcag gcgggacagc
     6481 gtccagcgtt tggcgatctc gatatagtcg cgcgggcttt tcgccttcac ttccgggccg
     6541 ctgttgacga tgcacaggct cttcagcagt tcggggcgat cgacgcccag ctgaaagcca
     6601 atcatgccgc ccatggagat gcccaccaga tgcacctgtg gtaattgcag gtgctcaatc
     6661 agcgctgcca catcctcggc aaaaccttga atgctgtagc gctcgcgggg tttgtccgag
     6721 cggccgtggc cgcgcacatc gagggcgata acctggtagt gacgagccag ctcggggatc
     6781 tgatattccc agtcgcgggt actcgaaccg aggccgtgga ccagcagcac gggcgcgccg
     6841 tggccgtatt cctcgtagtg cagctggcag tcgtcgttat caaaaaatga catctacagg
     6901 ttcctctcag gcggagggca ggggcgcagc aaaggctgca ttcaaagggg cggcatcgaa
     6961 gcgctggatc agctcgacga gaatctgcgt ggccgggccc aagggtttgt ctttgttcga
     7021 gtacaggaag aacagcggat tacggctgcc gccctgatcc agcggtaggg gcttgagctg
     7081 gccatccttg agttctcgct cgatcaggtg acgcggcaac caggcaaagc ccaggccatt
     7141 gctgacaaag gtggcggcgg tggccaggct gccgaccgtc cagcgttgtt cggcgccgag
     7201 ccagccgaca tcgcgcggct gctggcgccc ggaatcgcgg attactacct gcatctgggt
     7261 ttccagatcc tgaaaggtga cgacgcgctg cagacggtgc agcgggtgat cggggtgggc
     7321 gacggcgaca aactccacgg tgctcatttc cgcgccaaga aaaccgggaa tgttcagcgc
     7381 gctgatggcc aggtcagcca tgccgtcctt gagcacttct tccacgccgg acagcacctc
     7441 ctcgcgcagg cgcacccggc aaccacggct ctgcggcata aaggcggtca gtgcgcgcac
     7501 caggcgggcg ttggggtagg cagcatcgac caccaggcgc acttcggcct cccagccctg
     7561 ctccatatgg tgggcgaggt cttccagttg gctggcctgt ttgaccagtt gccgcgagcg
     7621 gcgcagcagt acgtcaccag cttcggtcaa caccgccttg cgcccgtcga tacgtagcag
     7681 cggcacgccc aactgctcct gcatgcgcgc cacggtgtag ctgaccgagg attgcgagcg
     7741 gtgcagcact tcggccgcct gagcaaaacc gccgtgatcg accaccgcct gcagggtgcg
     7801 ccattggtcg agggttacgc gaggggcttt catattcagt tcctgtgctc actcaagttc
     7861 ctgggtgtaa atagcgactc aatcaaccta aactggccgt cttttgccgg agactgcctg
     7921 atgaaaaagc tctgttgcgc actgctggcc tgcctgccgc tgactgtaat ggcctacccg
     7981 atcgaggtgg agaaacagct caatggcgct gaagtttcgg ccaccaccca ggaaatcgac
     8041 cacaacatgg gcggtttgca gctatacaac tatggccgca gcgatgccaa gtgtgcggcg
     8101 gtattccgca acggcccgga agcgccacgc acccgcaagg tcagcctggc tgccggtgaa
     8161 agcagcaacc tagcggtgaa gttcagccgc agcattatca aattacgggt caagctgacc
     8221 tgcgagctca gctagacagt ccttggccgc aacagcccag agactaagcc tgggctggct
     8281 ataaatcaaa ttattagatt gtttggcgca gatatttgcg ctttttaatc ggattgtgcg
     8341 ctcctattct ttgcccatcg acgttcaaca acccttcgat ggagcaaaca acatggcaaa
     8401 cgtcctggtt attgaaagca gcgcccgtca gcaaggttcg atttcgcgtc agctgactgc
     8461 gcaatttatc gccaactggt cggctgccaa cccagctgac cagatcaagg tgcgtgacct
     8521 tgctgttgat caggtgccgc acctggacgc caacttgctg ggcggctgga tgaccccggc
     8581 cgggcagcag agtgaggccg agcaggccgc gctggcgctg tccaacctgc tgaccgacga
     8641 attgttggcc gccgatgtgc tggtgctggc tgcgccgatg tacaacttcg ccatccccag
     8701 caccctcaag gcctggctcg accacgtgct gcgcgccggt gtcaccttca aatacagcga
     8761 aaccggcccg caaggcctgc tcagcggcaa gcgtgccttt gtcctgaccg ctcggggcgg
     8821 catctacgcc ggtggcactc aggatcatca ggaaccctac ctgcgtcagg cgctggcctt
     8881 tgtcggtatc cacgatgtca gctttatcca cgccgaaggt ctcaacctgg gcggcgactt
     8941 tatggaaaag ggcctgaacc aggccaaggc gcaactggca caggccatct gattttccgc
     9001 tgcatcctgc tctcctggcg tccggtgctc ctggacgtct ctgcccgatc tgctgatgca
     9061 gtgcgggctt ttttaggtct gcctatttgc gctgtacggc tgtttcaatg catagctgtg
     9121 tcgctttgtg gctgtggtct taactacaag ccaggcaaga tgccggggtc tgattgagca
     9181 gggtggagcg atgcagctgt ctgggcgcgg tgtggcgctg tcgattattg cctcggtgtt
     9241 gtttgccgtg gtgcccggct atgtgcggct gttggcaccg ctggatggct tgcaggtgtt
     9301 tgcccagcga gtgctctggt cgatgccggc agtgttgctg ctgattaccc tgtcgcggca
     9361 atggccggtg ctgctcgccg cctgtggtcg agtgcggcgc gagccgttgc tgctggccag
     9421 cctgccgttg gctgcgctgc tgatgggtat tcagtgggcg ctgtttgtct gggcgccgct
     9481 ggctgggcgc atgctggaag tgtcgctggg ctattttctt ctgccgctgg ccatggtgct
     9541 ggccgggcgg gtgttttatg gcgagcggct gcggccgttg cagcgtttgg cggtgttctg
     9601 cgccttgctc ggcgtggctc atgaactgtg gctgacccag gcgttttcct gggtggtgat
     9661 ggtcacggtg ctgggctatc cgccttattt tatgctgcgc cgctggatgc gcctggacgc
     9721 cttgtccggc tttgccttcg agatgctgtt tatggccccg gtagcgatct ggctggtgct
     9781 gcaatggagc ccgccgcagc tgttcagcca ggcgccgcag ctgtggtggc tgctaccggg
     9841 gctggcgatc ctcagctccc tggcgtttgc cgcgatgatg gctgccagtc gcctgctgcc
     9901 gatgggcctg ttcggcattc tcagttatgt cgagccggtg ctgctgtttc tggtggcgct
     9961 gctgtttctc ggcgagcagt tcaatagcgc gcaatggctg acctatctgc caatctggct
    10021 ggcggtgctg ctggtcggct gggacagtgc gcggttgttg ctcaagcagg cgcggcaagg
    10081 cttataggtc aatgcccgca gggttttccg gtagcacctg ataatgcagc tgcaccacgc
    10141 cgctgctgaa gctgcgttgt tcgtgcagcc tcaggctgcg ctccaggccg ctggagaaca
    10201 tcggaatacc ggcgcccagc aaataaggca ctaagctcac caccagctcg tcgagcaggc
    10261 cagcggcaaa gcagttgccg gccagcgagc cgccgccgac cagccagacg cgctgatggc
    10321 cttgctcggc cagctccgtg agcgcttcga tgggcgtgca gtggtgcatg ctcacgccct
    10381 caggcaattg ctcgtgcggg tagcgggtca gtacccggca gggtttgccg gggtagggcc
    10441 aggaaccctc ggacgccatc agcgtattaa aggtcatgcg gcccatcagc agggcatcaa
    10501 tgccgtcata gaaggcgtgg tagccgtggt cgtcgccaga ggcctggatg gcttccagcc
    10561 aatcgactct gccatccggg cgagcgatat agccgtccag gctggcggcc acgtagtaga
    10621 tcagggtggg tttcacgcgg gctcctccaa acgaccgaac gagcccaggg caagggctgc
    10681 tgaacggttg agtcggattg cccggcaaag tttcctgcta tttggcgcac gcttagctgg
    10741 tggtctgagg gcgtccggcc agtatgcgca ggatggcatt gagcaacatg ccgtagagcg
    10801 gcacgaacag cagcaggctg accgccagtt tgaccccgta atccacggtg gcgatttcca
    10861 cccagtgttc ggccataaac gggttgctgc tgttccagaa cgctacggag aagaacgcgt
    10921 aggtgtccag caggttgccg aaaatggtcg agacggtcgg cgccacccac cacagcgaca
    10981 gctggcgcag gcggtcaaat acctggatat ctaggatctg gcccagcaca taggcgagga
    11041 agctggctat ggagatacgc agaacgaact cgttgaactc cagcagtgcg ccgaagccgc
    11101 ggaagctggc ttcctggaac aataccgaga cgatgtagga aattagcagc gccggcagca
    11161 ttacttgggc gatcaccagg cgcgccgtgc gtttgccgag cagacgcacg gtcaggtcgg
    11221 tggccaggaa aataaacggg aagctgaatg cgccccaggt ggtgtgccag ccgaacaggg
    11281 tgatcggcag ctgcaccagg tagttactgg cgatgatgat caggatatga aacagcacca
    11341 gggccataag cgcattgcgc aggcgctgag gagcggtaat aagcatgaca gaaccttttt
    11401 gctgaatggg gttagggaac ccatgccgtc cggaattgaa cggcggcgcg cattctaccg
    11461 gcgcgacgcg gcgcttgcgt agtggcctga cgggaggcct caatctgcgc cgttttagcg
    11521 tcctgctgca gggcagccaa ctaggctaca gacgccgttg gccaggccgt tcgggctttt
    11581 agccggcaga accggtaatc tatgccgctg tattgctgat tttttgtcga ggtatacccg
    11641 tgttttccca attcgccctg catgaacgcc tgctcaaagc cgtggccgag ctgaaatttg
    11701 tcgagcccac cccggtgcag ttggcggcca ttccgctggc gctacaaggg aaagacttgc
    11761 gggttaccgc gcagaccggc agtggcaaga ccgcggcctt tgtgctgcca atgctcaatc
    11821 gcctgctcgg cgatggtgct tcccagccac gcatgagcgt gcgcgcgatg atcctgctgc
    11881 cgacccgcga gctggcgcag cagaccctca aggaagtcga gcgctttgct cagttcacct
    11941 tcctcaaggg cgggctgatc accggtggtg aagacttcaa ggtgcaggcc gccatgctgc
    12001 gcaaggtcga catcctgatc ggtacgcctg gccgtctgat cgaacacgcc aacgccggca
    12061 acctgctgct cgatgaagtc gaagtgctgg tgctcgatga agccgaccgc atgctcgaca
    12121 tgggctttgc cgaggacgtg cagcgtttgt ccgagctgtg caacgccgac cgtcagaccc
    12181 tgctgttctc cgccaccagt ggcggcgccg tgctgcgcga agtggtcggc aaggtgcgga
    12241 aagagcctga gcacctgcag ctcaaccgcg ttagccagct caacgaaggc acccgtcagc
    12301 agatcatcac cgctgaccac aaccaccaca aagagcgctt ggtcgagtgg ttgctgaaga
    12361 acgagaccta cgacaaggca attatcttca ccaatacccg cgtccaggct gaccgcctgt
    12421 acggcaagtt ggtcgcccat gacttcaaaa cctttgtgtt gcacggcgag aaggaccaga
    12481 aggatcgcaa gctggccatc gaccgcctca agcaaggtgc ggtgaagatt ctggtggcca
    12541 ccgatgtggc ggcccgcggc ctggatgttg acggcctgga tctggtgatc aacttcgata
    12601 tgccacgctc cggcgacgaa tacgtgcacc gcatcggtcg caccggccgt gctggcgctg
    12661 aaggtctggc gatctcgctg atctgccata acgactgggc gctgatgtcg agtatcgagc
    12721 gctacctcaa gcagcgtttc gaacgccgca atatcaagga cctcaagggc atctaccagg
    12781 gcccgaaaaa cctcaaggcg tccggtaaag cagtcggccc gaagaagaaa aagatcgacc
    12841 cgaaaaccgg caagaagctc gccacgaaaa agccggccgc caaggcgccg aagcgcaaca
    12901 ccatcaaccg gccgaaaccc gatgcgccgg tgctggtcag ccaggacggc ctggccccgc
    12961 tgaagcgccg cgctccagcc gccgagtaag ccaatggttt ggcgtctggg tctgttcgcc
    13021 gcgctggctg ttgccgcgca ggcgcagggc gcggtgcaca aatgcgtgca gcctgacggt
    13081 gtggtgctct acaccgatca gccctgcagc attgcccaca gcaacctcgc cttgagtgtc
    13141 agcgactacg ccagctaccc gccgttactg cagggtgttg cgcagctctg gcacgaacgg
    13201 gttcagccgc tgctggtcgg ggtcgatgtt ctggccctgc tggcgctggt ttacgggctg
    13261 atgagcctgg tctgctatat cgcctactac cgcgacaagc agttcgccat caagggccag
    13321 cagcgcacgc ccgaggcgcg cctgcatctg tatgagttgc tgggtggctg gccaggcggc
    13381 ttgctggcgc agcggctgat tcggcacaag aaccgcaagc tgagttatca gctgctgttc
    13441 tggttgatcg tcctgctgca cctgtccctg gcggggctgg tgctgtggtt gatcaatacg
    13501 cctgcaggcg gctgaagacg gcgggctttc cgccgtctgc cgcttgatcc ttacttcggt
    13561 acgatcagga tcttgccatc gcgttcgcca ctggcggcgg ccgctacggc ctgtttgatc
    13621 tcgctgacgt cataggtggc agcaacccgg gtgtgcagct tgccgctggc gatcagctgt
    13681 accaactcac caaatacctt catctgctcg gcctggctgg cgttctggaa ccacttggcc
    13741 aaccagaagc cgcgcaggct gacgtcgcga aacacgaagg acgctggcga tacctggcac
    13801 ggctggcggc tcatcatgcc gtagttaacc agcacgccgc cgtcgcagag gctggcggcc
    13861 aggtgatcgg tagactcgcc gcccacggca tcgataccca agcgtacatc tgcgccgccc
    13921 gtggctgcgc gcacgcgctt ggccaggtcc gggccatcga ccagcaccac gtcaccacct
    13981 tctgcctcaa cgccggccac tgccgactca cgacgtacca cgttgatggt tttgaagccg
    14041 cgcagcttgg ccagttggat caggtagctg ccgacgccag agttggcggc gttctggatc
    14101 acccaatcgc cgggcttgag gtcgacgaac tcactgagca tcagggatgc ggtcggtgga
    14161 ttgaccgtaa gcatcgccag ttgctgcgga tcagcttccg gcaacgggat caatttgttc
    14221 gcaggcgcat tcaggtggct gacccaggtg ccgcaaccca ccggcagcaa caccatctgg
    14281 ccgaccttca ggttgctgac ctcggcaccg agcgcttcta cccggccaac tccttcgttg
    14341 ccaccaatcg ctggcagcgg cggcagcatg ccgtattcgc cggtcagggt cagcacatcc
    14401 gacgggttga tcggcgcagc cagcaccttg atgcgcactt ggcctgcgcc aggctccgct
    14461 agctgcagcg caacggcggc gattgcttcc tgaggaacgg gcccgcgggt ttggtactcg
    14521 gctttaagca tgggaattct ccggtagggg ctgaacaaag tggctgaagt ctagaccgga
    14581 gtggccaagg tgcgacgaat cttccgttat cgatccgggt gattggcagc ggctaagcaa
    14641 accgaatgac ggctagtttt ggctgtcttc aacctcttcg ccggcctctt ccaattccaa
    14701 gaggcgctgg tcgtagcctt cgcaagcgtt ttccagctcg tcagcagacg tctcgggcga
    14761 ggtttcctta agttcttcgc gcagctgcgc tgcgcgggcc ggatcttttt tggtgatctc
    14821 tgcgactttc gccgcgagtt ctccggcttt ggcggtcgcc tcttcggtgg tgcaggaggc
    14881 gtaggtcggg ctctgggcaa ataaagcgaa gacggcgcaa aggccgatgg cggttttgct
    14941 gggcaggttg gtgttcatgg caatgtcctc gcgggcggaa cagctggctt atgcggttga
    15001 gtccgcttga ccgaggaagt tcaggcgtta tggggccgct acaggtggtc ctaacgcacg
    15061 cggtcgcggc ttttcaccgc aagatcgagg gccttctgca tatcgagtcg cgcggagcgg
    15121 ccgatgtccg ggttatccag ctcgtagttg cgctcggagg gcagaatcgg cgcggtcagg
    15181 tcttcggcat cggtcggttc gaagtcgaac tcttccccca gggtcatggc gcttcggcgc
    15241 aacagcatgc gcagctgggc ggggtgcagg tcggggttga tcgacagcaa tccggcgacc
    15301 aggccggcga ccagtggagt ggcgtaagag gttccacaat ggatatcgcc ttgctcgttt
    15361 gcgcctatgg tcgaggcctg cacgcaggca gcggcgctga tgtcgacgcg ggtgtcgcta
    15421 ttcgatgagc tgcgtttgat cgcgtaatcc gcatccgtca ccggcacatc gcggtcgcta
    15481 cgctgatgcc cgccaaccac cagcagctga tcggtgataa acgaggatgg cagtcggtat
    15541 tcgtcagcgc cggagaacga ggcaccgttg ccagccgagt tgaccacaac cacctcggga
    15601 tgctcgctgc gtagccataa gaaaaactct tcgagcagtt cctcatagcc gctcatggcc
    15661 agccctgagc gaaccagcga atcgacttca tcaccacgaa tatttttcgc cccaatgcga
    15721 tgaaggcccc agctccagtt gagcacccgt acaccgtctt ccaccagatt gaccgacgcc
    15781 gcgatgttgg cagtgatgcc cgcatccgaa ttgcgctcga cgatcacctc gaaaccgggc
    15841 ccggcaccat ctaagccccg caggaagccg gtgttgccgc ccttatccca gcgcgcggcg
    15901 agaatgcccg cgacggtcga gccatgtcca tctggccgcg cggcatcgcg ggcgtacagc
    15961 cgggtatgcg gtttgccatc ggcgcgctca gcggcgccaa gatagtcatt gaagtcggcc
    16021 gcatcaaagt ccacatcacg ctcgatcagg ccgatgcgca ccggtgtggt cggtatgggc
    16081 gagttcttgg cgggaacgcg tcgttggtaa taattgaccg cgtcgagaaa acgattggcg
    16141 gcccactcgt tctggtgcgg tgtgttggcc gcaggattgt cctgtgtcac tgcgtcgcct
    16201 tcttccgcgc cggactcctc gatcaccacg gcgtccacgc tgacttcact gcccaggcgc
    16261 agcaccatgg cgtcgcgctc gatgagattg tccacgggta agcgcaactg ataggtgttg
    16321 agcggggcaa tggtacccac tacggtggcg ccgtatttct gtgcgagctg acgcgacact
    16381 gccaggccgt cggcgtcttc ctcgataatc aggctgatca gatcgacata ggtggtcagc
    16441 ccatccatgt tcttcgccac ttcgtcgggg ttggcggcca ccacgtggct gcccttgagc
    16501 gacagccaca ccggattgct ggcctggccc tgctgctcca gccagattgg tccgctttgt
    16561 tggcggcctt gagcaatagt cacctgtatt ttcttgccgc tgcgttctac tgccgcgctt
    16621 ggcagcggcg cgccatcgaa catcagtttg aacgcgccgc gctgcaaacc ctggctctca
    16681 aggcagaact gctgctggtg cttgtcgaac agatcgccgc agcgctgcag gtgcaccaga
    16741 cgaagtggcg cttgcgcctg aacggcggca gtgatgatca gcaatgtaat ggccagtaac
    16801 ggctgtgaca ggcgcatggg tacacaaacc tcgacacgtg acaggtgccg aatgtctgac
    16861 tgctgctgca agggcatggt tcagggtgtg ctgtggtgtg gttctgcttc aggtcgaacc
    16921 taagcgggta tgggtgggtc cgaaaccaag agggcgacgt gcaacgagcc cctcgcgctt
    16981 gccaacgggt cgagcgcaac gcggatggga gaaggtgata tgtcttataa ctgggatctt
    17041 atcgagcgct tgctgcatga ggtgcaaaac tctgccggta aaaatttcgc gcctcgggct
    17101 tatgccgagg aactcgcgca ggagctggag cgcgccggtg agcacaccag caatctggat
    17161 gccttgaaag ccgaggcgac agaatatgag tcgcagctgc tcagtgcggg gtttatcgaa
    17221 ccacggccgg aagaagaggg cggcaacggc gagaatttcg tgctgacgcc acgaggtttg
    17281 cagctgctca gcatgatcga tagcagcatc cctggggggg aacactcccg tgagctgctc
    17341 gacgaccaag gcacagcagc tctggtgccg gaggtttttg acgagacggc gggcaaagcc
    17401 aatctcgttt gatggccctt ggctatttac cggccagcgc gtcagggttc tgattttgtc
    17461 tggagtacct ggcgtatgcc taccgtgcag accggcccct gcacgactgc ttaatcgacc
    17521 gcagtgatag agcaatgcag agtcacgcca tagccggcta tgctggtcac tggtttaact
    17581 cgagtattgc catgaagtgg ttgtgggtct gttgtttgtt gtcatgcagc ctgacggtgt
    17641 cggcgcaagg cctgcgcgtc ggctttggca cgcacaaacc gccgtatatc ttcgagaatg
    17701 aagcccgcgg cctggagcac gatattgtta tggccgccgc gcaacgcggc gggttggaac
    17761 cggtggcgta ctacgcaccg atggaacgtc tgaacctgat gctgagcaaa ggtcagattg
    17821 acgttattgc caccaccaat gagcgcagcg gcggttcaat tttttattcg gatgcctata
    17881 ttcgctacca gaatgtcgcg gtcgccttgc gctcgcgaaa cctggatatc cagcgcatca
    17941 gcgacctggt gggctattcg gtcaatgcct ttcagcgcgc gcgctttctg ctcggtagtg
    18001 agtaccaggc catggccgaa aacaacccac gttaccgcga ggaagcgttt cagattgccc
    18061 gcaaccgcat gctctacagc ggccgagtgg atgtagtggt gacggacatg cgcatcctgc
    18121 gttacttcaa ccgtgaggtg tacacccagg ttgatgtgac ccagccgttg gccctgtatc
    18181 cgatttttgc ggccaccgac tataagctag gttgccgcca gcaggcccat tgcgagcgct
    18241 tcaatcaagg cttggcagaa attcgggaga gtggcgagta caggcttatc gagcgccgct
    18301 acgcgatgca ctgatacacg tgcatcagcg ggttatgggg tgggcgcgac ctcgtcgagg
    18361 ccttcttgcg ccgccaacag ttcttcctcg gtctgcagcg gcgtgctgct gacgctgaaa
    18421 tcgataatct cgatggaacc ctggccctga cccagcaggt ggaaggagaa cgccttacgc
    18481 gcttgcgggt tgtcgaaggt aaagctgacc tcgacgggct tgttacgggt tagtagcggc
    18541 agctcgggga tcgccaagtc gacgttgcgg tcgtattcct tggttttcaa ttgcagcgtg
    18601 gcgccgtgtt tatccatgcg cagggcgcgg attttcaggg tcacggtggt ctgggtagct
    18661 gcacccaggg tcaggtactg cgcacccact aggttgtctg cccagtcatc actgacactg
    18721 ggtttgagct tgattggctg ctggtgggcg aactggtagc gctgctcggc ctggccaccg
    18781 ttgctcaggg attggtcgag cagggccgct tgctggctga tcaggcgtgc cggtttgccg
    18841 ctgaaacggc ccaggtagcg cgcgctgtcg gcaataaagc cagggctggc atagcgccgg
    18901 cagacattct ggaaatcgca ttcggtcagc gtgccctggc cgtcatgccg gcgtagcagg
    18961 ccattggtgt aggagatgat ctcgcggccc gtggcgtaat cgcgcagcaa tgagcgcccg
    19021 gcaatattgg ccggtaccgg gaaggcgaag tagtcgagca cagaggcggt caggtcaaca
    19081 tggccataca cgccctgctt gagtgccggc agttgcgcct gttcgggagc tagcagcagg
    19141 ttgaagcccc aggtcgaggc cagacgtacg ttttccaggc cgtgggattc atccgaggtg
    19201 acgatcacca gggtgtcttt catcaccccc tgtttttcca aggcgttgag gaagtcggcg
    19261 acggcgtcgt ccaggtaggc aatcgccgcc agtttggcgt tggggtgacg gtccaggtag
    19321 tcctgcgggg cggagtaggg ctggtgagtg cccacggtca gcaaggtcag catccaaggc
    19381 tggcgcttct tgcgcagctg tttcacgtaa cccagcgcgc cttcgaagta ggccttgtcg
    19441 tccatgcccc aagggaactc gagatagggc ttgttcttga accagtcgcg gccgagggtt
    19501 ttgtcgaagc ccatctgcgg catgatctgg tctttggcca taaagcgcag cccggcacct
    19561 tgcaggaagt gtgtgctaag gccgcgttcg cgcagttgcg cgggcaggca ttgctcgctg
    19621 cgcgccgggt tgttgagcag ctcaacgcct ttcggcgtgc cggagtcgag tttgttgtaa
    19681 tcgccgcaga gcatggcgta gaggccgcgg atggtctgat ggccgtgcag cacataatcg
    19741 gtggtgagca tgccgcgctc ggcccattgg ctcaggcgcg gcattggatc ttgcgtgtag
    19801 ctgctgccga tagcctggcg actggcttgc aggtaggcgc cggtcacgcc ttccagggta
    19861 atgatcagca cgttgcgagc gctgccggcc tgcttgagca gcggcgtgcc gctcagatcg
    19921 agttggttca gaccgctgat gtcgggtggg ctgctgggct catcccccgc cagccagtcg
    19981 ctcaggtgct gttgagcgct attgctactt tccgccagca gcttgtgcgg caggttgaac
    20041 tgcagccatt ggtccgcttc gctgggtttg aattgttggc cgacgctgtg cacggcaaac
    20101 agcgccagcg gcagcaacag ccaggtgcgt gcaagcggca ttgcactgcg cggcgtttgt
    20161 acgctgcgca ggcctaggta aagcgctgcc agcacggcca tggccaaagc cagtaacggc
    20221 tggctcaaac cgctgccctg ggttgaatgg ctgacgaact gcgcatcgcc caggtattgc
    20281 aggtcggccg gctccggcat gcgccccacg gcattgacca gctcggcggt gctcagggta
    20341 aagatgcacc aggccagcag cagaggaatg ctcagcagcc aggagcgttg gtgcagaaca
    20401 accagcagca ggctgccgat ggccaggtca gacagatagc cataggcgtg cgaccagccc
    20461 agcgtgtagc ggctgagcaa cggcagggcg agtaataaca aaccgagggt aagcagggtg
    20521 ctggaagggt gtcgcagcca attgcaggta acgcgcacgg gtactcctgg ggcgattgcc
    20581 atcgttcagc ggcggcaatg ccgaaatcat ccgcaagatg cggactgaca tgattctgaa
    20641 acatggttgt gctaagcgcc agtaggcggg tcgcaaaagg gcaaaacaac ttatgtcgcg
    20701 aggaggaggg aaacggggga ttgggagggg ttatgcactg gccagcgcct tgtcattgcg
    20761 agcaaagcgc tgcatggcca gagagcgtta ctttttgccg atggtgatct gcttggtggt
    20821 gccgtacacc tggccgctga cgcctttggc aatttgctga atttccccgc cggatttcag
    20881 gaaggcttcg atctgtgcgt cgatcgaggc gctggtttcc acggctggag ccggcttg
//
LOCUS       DASRUM010000253         4122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1128889, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000253 DASRUM010000000
VERSION     DASRUM010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128889"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4071)
                     /locus_tag="VFF22_17090"
     CDS             complement(<1..4071)
                     /locus_tag="VFF22_17090"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZX18570.1"
                     /translation="MKIPFTLGLRGRLILLLLAAFAALSGQIVWYSIENRDERLSDAS
                     EHLLRNTQLIAARQQRITARADAVLTGLILRPDLRPDVSTETCSRTLAAILQQEPTFV
                     QVGMTRHDGVVACAAVPPKGQVSFADRNWFQSALQSHDMVIGDVVTGRIVGKPIITFA
                     KAQRDEAGRVAAVFYLSLNLEWLRDELATARLPEGSRLVVVDAKGTVAVRHPDPEGWV
                     GKSAENLPLWRRIQAAGGEGVAEDSGLDGVHRVFGFTPLLDTVSGRMTLWLALPKAVV
                     EAPVQRQLWLSLAFALAVLVATLGLAILGGNRLLLRPLLALARAAERHSAGDLGARSG
                     LPHTADEIGWLARTLDESAATVEDREHRLAYANHALRVLSAGNRTLLRATSEQELLEE
                     MCRAIIEAGGYRLAWVGFAEHDKRVKLVASWGVEADFLASLNLTWDETAAGQGPTGTA
                     IRRGIPIACSNVQTDPGYAPWREQAQRYGYASSLALPLRLDGAVIGALCICSAEADAF
                     DAEVVELLSEAANDLAYGIATQRAKAEHKRTQTELQRLERQNTLILDAAGEGIFGLDM
                     EGRATFINPAGAAMLQWTAEEIAAQTMHALHHHTRADGTPYPREACPIHAAYRDGAVH
                     RVTDEVFWRKDGTSFPVEYVSTPLRDERGELAGAVVSFSDVSKRKQAEEKLRETRDYL
                     ENLFNHANAPIIVWDPSYQITMFNHAFEHLTGRDANEVIGKQIDTLFPADRKDEALSH
                     IYQTRTGERWETVEIPILHSNGEVRTALWNSATLYSADGKSVVATMAQGQDITERKAA
                     EEQIRKLSLAVEQSPESIVITNLDAEIEYVNEAFVRATGYSREEAIGRNPRILQSGKT
                     PRATYDALWQALTHGEPWRGEFINRRKDGSEYIEFAIITPIRQPDGRITHYVAVKEDI
                     TEKKRLADELDQHRQHLEELVETRTHELAQATIAAEAANQAKSAFLASMSHEIRTPMN
                     GVIGMIDVLQQSSLNSSQMESANIIHDSAYSLLAIINDILDFSKIEAGKLQIDSLPMS
                     VAEVVEGACKTIDHMALKKNVELTLFTDPAIPAAVMGDAGRLRQILINLANNAIKFSS
                     RQTRQGKVSVRAVLVESGPDGQSRFPLPNPLEIPSPQSSEGSTSHSTKHDEAVQVAGY
                     PASGRGGEREEQLSIPESLRDVEQILLEFRVTDNGIGMDEQTQARLFTAFTQADSSTT
                     RTYGGTGLGLAISGQLANIMGGKIAVQSEPGKGSMFSVRLPFKLAPDVGRMSVALSAE
                     GDCRKALCFSDLQNLPCLVVGGAESLADDLAAYLEYDGALVERVEDLAAANKWIAGRT
                     PGLCVVVIDTAGSPPLATPLLDGLRVAARAHPEQQTR"
BASE COUNT          676 a         1309 c         1320 g          817 t
ORIGIN      
        1 gcgggtttgc tgttccgggt gggcgcgggc cgcaacgcgc aggccatcca gtaacggcgt
       61 ggccagcggc gggctgcctg cggtgtcaat gaccacgacg cacaggccgg gtgtgcgacc
      121 ggctatccat ttgttggcag ccgccaagtc ctccacccgc tcaaccagcg caccgtcgta
      181 ctcaagatag gcggcgagat catcggccag actctccgcg ccgcccacta ccagacaggg
      241 caggttttgt aggtcggaaa agcatagcgc cttccgacaa tccccttcgg cggataacgc
      301 tacgctcatc cgccctacgt ccggtgccaa tttgaacggc agccgcacac tgaacatcga
      361 gcccttgcca ggttcgctct gcaccgcgat tttgccgccc atgatattgg ccaactggcc
      421 agagatggcc agccccaacc cggtgccgcc gtaagtccgg gtagtggatg agtcggcctg
      481 ggtaaaggcg gtaaacaacc gcgcctgagt ttgttcatcc atgccgatgc cgttgtcggt
      541 cacgcgaaac tccagcagga tttgctcaac atcgcgaagc gattcaggta ttgataattg
      601 ctcttctcgt tcgcctcctc tcccgcttgc gggataacca gcgacttgca cggcttcatc
      661 gtgcttggtc gaatggctag tagatccttc agaggattga ggagagggta tctccagagg
      721 gttggggagg gggaaacgac tctgcccgtc cggcccactt tcgaccagca cggcgcgcac
      781 cgacaccttg ccctgcctcg tctgcctgct ggaaaacttg atggcgttgt tcgccaggtt
      841 gatcagtatc tggcgcaacc gccccgcatc gcccatgact gctgcgggaa tggcagggtc
      901 ggtgaacagc gtcagctcca cgtttttctt caaggccatg tggtctatgg ttttacatgc
      961 cccttccacc acctcggcga cagacatcgg caggctgtca atctggagct tgccggcctc
     1021 gattttggag aagtcgagaa tatcgttgat gatggccagc agggaatacg ccgagtcgtg
     1081 aatgatattc gccgactcca tctgcgagct attcaggctg ctctgctgca acacgtcgat
     1141 catgccgatc acgccgttca tcggggtgcg aatctcgtgg ctcatggagg cgaggaaggc
     1201 cgacttggct tggttcgccg cttcggccgc aatcgtggcc tgggccaact catgcgtccg
     1261 ggtttcgacc agttcttcca ggtgttggcg gtgctgatcg agttcgtcag cgaggcgctt
     1321 cttctcggtg atatcttcct tcaccgcgac atagtgggtg atgcgcccgt cgggctggcg
     1381 gataggcgtg atgatggcga actcgatgta ttcgctgccg tccttgcggc ggttgatgaa
     1441 ttcgcctctc cacggctcgc cgtgggtcag tgcttgccac agggcatcat aggttgcgcg
     1501 cggcgtcttg ccggattgca gaatgcgcgg gttccggcca atcgcttcct cgcggctgta
     1561 gccggtggcc cggacgaaag cttcgtttac gtactcgatt tcagcgtcga ggttggtgat
     1621 aacgatgctc tccgggctct gctcgacggc aagggaaagc ttgcgaatct gttcttccgc
     1681 cgccttccgc tcggtgatgt cctgcccctg cgccatggtg gccactacgc tctttccgtc
     1741 agccgaataa agcgtcgcgg aattccacaa agctgttctg acctctccgt tcgaatgcag
     1801 tatcggtatc tcaaccgttt cccagcgttc gcctgtcctg gtctgatata tatgagacag
     1861 tgcttcatcc ttgcggtcgg caggaaacag ggtgtcgatc tgtttgccga tgacctcgtt
     1921 ggcatcccga ccggtcaggt gctcgaaagc atggttgaac atggtgatct ggtatgacgg
     1981 atcccacaca atgatcgggg cgttcgcatg gttgaacaga ttttccagat agtcacgggt
     2041 ctcccgcagc ttctcttccg cctgcttgcg cttgctgaca tcactgaagc tgaccaccgc
     2101 gcccgccagt tcgccgcgtt cgtcgcgcag gggtgtgctg acatattcga cggggaagct
     2161 cgtaccgtcc ttgcgccaga acacctcgtc ggtcacgcga tggacggcgc catcgcggta
     2221 ggcggcgtgg atggggcagg cctcgcgcgg gtagggcgtg ccgtcggcgc gggtgtgatg
     2281 atgcagggca tgcatggtct gggcggcaat ctcttccgcc gtccattgca acatcgccgc
     2341 gccggccggg ttgatgaagg tggcgcgccc ttccatatct aggccgaaga tgccttcgcc
     2401 ggccgcgtcg aggatcagcg tgttttgtct ttccagccgt tgcagttcgg tttgggtgcg
     2461 cttgtgctcg gccttggcgc gctgcgtggc gatgccgtag gccagatcgt tcgccgcctc
     2521 gctgagcagt tcgaccacct ccgcgtcgaa ggcatccgct tccgcggagc agatgcagag
     2581 cgcgccgatg acggcgccgt ccagccgcag cggcagggcc agcgacgagg catagccata
     2641 gcgttgcgcc tgttcccgcc agggcgcata gcccggatcg gtctggacat tgctgcaggc
     2701 gatgggaatg ccccggcgga tcgccgtgcc ggtcggcccc tggcccgctg cggtttcgtc
     2761 ccaggtgagg ttcaggctgg cgagaaagtc cgcctcgacg ccccaggagg ccaccagctt
     2821 cacacgcttg tcgtgttcgg caaaccccac ccaggccagg cgatagccgc cagcctcgat
     2881 gatggcgcgg cacatttcct caaggagttc ctgctcgctc gtggcacgca acagggtccg
     2941 gttgccggcc gacagcacgc gcagggcgtg gttggcgtag gcgagccggt gctcccggtc
     3001 ctcgacagtg gcggcggatt catccagcgt ccgtgccagc cacccgatct cgtcggcggt
     3061 gtgcggcagc ccgctgcggg cgccgaggtc gccggcgctg tggcgctccg ccgcccgcgc
     3121 cagcgccagc agggggcgca gcagcagccg gttgccaccc aggattgcca gccccagcgt
     3181 ggcgacgagc acggccagcg cgaaggcgag gctgagccac aattggcgtt gcacgggggc
     3241 ttccaccact gccttcggca gggccagcca cagggtcatg cggccggaaa ccgtgtccag
     3301 cagcggcgtg aagccgaaaa ccctgtgcac tccatccaga ccgctgtcct cggccacgcc
     3361 ttcaccgccc gctgcctgaa tgcgccgcca tagcggcaga ttttcggcac tcttgccgac
     3421 ccagccctcg ggatcgggat ggcgcacggc cacggtgccc ttggcgtcca ccaccaccag
     3481 ccgggagcct tccggcaggc gggcggtcgc cagttcgtca cgcagccatt ccaggttcag
     3541 cgacaggtag aagacggccg caacgcgccc ggcctcgtcg cgctgcgcct tggcgaaggt
     3601 gatgatcggc ttgccgacga tgcggccggt caccacgtcg ccgatgacca tgtcgtgcga
     3661 ctgtagggcc gactggaacc agttgcggtc ggcaaagctg acctggcctt tggggggtac
     3721 cgcggcacag gccaccacgc cgtcgtgccg cgtcatcccc acctgaacga acgtcggttc
     3781 ctgctgcagg atggccgcga gtgtccgcga gcaggtctcg gtggagacgt ccgggcgcaa
     3841 atccggacgc agtatcagcc cggtcaaaac tgcatcggcc ctcgcggtaa tgcgctgctg
     3901 ccgggcggca atcagttgcg tgttacgcag gaggtgttcg gatgcgtcgc ttagccgctc
     3961 gtcgcggttc tcgatcgaat accagacgat ctggccgctc agcgcggcga acgccgccag
     4021 cagcaacagg atcaggcggc cgcgcaggcc gagggtgaaa gggattttca tgtcgctgcg
     4081 gcctgccatg gcgggctagc ggcgaccgtt cgcgctatgg cg
//
LOCUS       DASRUM010000254        18362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1642293, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000254 DASRUM010000000
VERSION     DASRUM010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 18362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18362
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642293"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..67)
                     /locus_tag="VFF22_17095"
     CDS             complement(<1..67)
                     /locus_tag="VFF22_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18571.1"
                     /translation="MYKLEVQDLHKRYGSHEVLKGV"
     gene            complement(86..1198)
                     /locus_tag="VFF22_17100"
     CDS             complement(86..1198)
                     /locus_tag="VFF22_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019581926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HZX18572.1"
                     /translation="MQRIDHPLPWGCPGTERRLSVFRFGQGPCKAYIQASLHADELPG
                     MRVAVELKRRLRQLEAQGRLTGVIELVPVANPIGLGQMFQATHQGRFEMGSGKNFNRD
                     FFDLAAAIAPALEGCLGSDGPSNVRLIRAAMQAALEALPPAGSELQGMQRLLLKHACD
                     ADVVLDLHCDFDAAVHLYLLPQQWPQLRSLAARLQAGAALTVEDSGGSSFDEACALPW
                     VHLARHFPQAAVPLACLAVTVELGGMADTGKPEAEASAESILAFLAEQGLIAGDWPPA
                     PAESCDATPFNGAQYAYAPHAGVVSFLQPVGARVKAGDPLFEVINPLDDRHTTVCAVT
                     DGVLYARERLRFAQPGLWLAKVAGRTPIRQGRLLSD"
     gene            complement(1209..1907)
                     /locus_tag="VFF22_17105"
     CDS             complement(1209..1907)
                     /locus_tag="VFF22_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX18573.1"
                     /translation="MIFDYNVILENLPLYFGGVLVTLKILLISLACGLALAIPLGLMR
                     VSKSPLINFPAWLYTYVIRGTPMLVQLFLIYYGLAQFEAVRESVLWPYFSSATFCACL
                     AFAINTSAYSAEILAGSLKATPHGEIEAAKAMGMSRFKLYRRILLPSALRRALPQYSN
                     EVIMMLHTTSLASIVTLIDITGAARTVSSQYYMPFEAFITAGLFYLCLTFILVRLFKL
                     AERRWLAYLAPRKA"
     gene            complement(1923..2612)
                     /locus_tag="VFF22_17110"
     CDS             complement(1923..2612)
                     /locus_tag="VFF22_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX18574.1"
                     /translation="MLNGYGSTILEGAWLTLILALTSMAVAIVLGLLGAAFRLSPIKW
                     LALLGETYATVIRGIPDLVLILLIFYGGQDMVNRLAPMLGYDDYIDINPFIAGVFTMG
                     FIFGAYLSETFRGAFMAIPKGQAEAGAAYGMNGMQVFFRILVPQMIRFAIPGFTNNWL
                     VLTKATALISVVGLQDMMFKAKSAADATREPFTFYLAVAALYLVLTSVSLLALRYLEK
                     RYSVGIKAADL"
     gene            complement(2732..3511)
                     /locus_tag="VFF22_17115"
     CDS             complement(2732..3511)
                     /locus_tag="VFF22_17115"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX18575.1"
                     /translation="MKKIALLGALALSMLSPLAMAEEAKPLRIGIEAAYPPFAFKTPE
                     GAISGFDYDIGVALCEEMKVECKWIEQEFDGLIPALKVRKFDAVLSSMSITEDRLKSV
                     DFSGKYYQTPGKLAMKAGTVLNDPLVDLKGKKVGVQRASTYDRYATDIFAPAGIEIVR
                     YSSQNEAFLDLTSGRLDATFADVVNIDDGFIKTDAGKGFALVGPDYTDEKYIGMGAGI
                     AVRKGDKALADKISAAILAIRANGKYKEVQDKYFDFNVYGD"
     gene            complement(4032..5987)
                     /gene="acs"
                     /locus_tag="VFF22_17120"
     CDS             complement(4032..5987)
                     /gene="acs"
                     /locus_tag="VFF22_17120"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HZX18576.1"
                     /translation="MSAASLYPVRPEVAAQSLTDEATYKAMYQQSVINPDGFWREQAQ
                     RLDWIKPFTKVKQTSFDDHHVDIKWFADGTLNVSYNCLDRHLEERGDQVAIIWEGDDP
                     SEHEEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPQAVVAMLACTRIGAIHS
                     VVFGGFSPEALAGRIIDCQSKVVITADEGLRGGKKTPLKANVDDALTNPETASIQKVI
                     VCKRTGSPIKWNQHRDIWYEDLMAVASSHCAPKEMGAEEALFILYTSGSTGKPKGVQH
                     TTGGYLVYAALTHERVFDYKPGDIYWCTADVGWVTGHSYIVYGPLANGATTLLFEGIP
                     NYPDITRVSKIIDKHKVNILYTAPTAIRAMMAHGDAAVAGADGSSLRLLGSVGEPINP
                     EAWNWYYKAVGKERCPIVDTWWQTETGGILISPLPGATALKPGSATRPFFGVQPALVD
                     NLGNLVEGAAEGNLVIIDSWPGQARTLYGDHDRFVDTYFKTFKGMYFTGDGARRDEDG
                     YYWITGRVDDVLNVSGHRMGTAEIESAMVAHPKVAEAAVVGVPHDIKGQGIYVYVTLN
                     GGEQPSEQLRQELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRKIATAEYD
                     ALGDISTLADPGVVQHLIDTHREMQVA"
     gene            complement(6130..7128)
                     /locus_tag="VFF22_17125"
     CDS             complement(6130..7128)
                     /locus_tag="VFF22_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZX18577.1"
                     /translation="MTSSFVRRGLVGIVAGCALIGALSAQAAESQPTAPILIKFSHIT
                     ADTTPKGQGALLFKKLVEERLAGKVKVDVYANSSLYGDGKEMEALLLNEVQMLAPAPS
                     KLEQYTKQLQLFDDVAAAQRFQASDKGKALLKSMESKGITGLAYWLNGMRQLTANKPL
                     REPADARGQKFRVQPSDLQAAQYSALRAVPRKMSFAEIYQGLQTGVVNAQDNPWSNIY
                     SQKYFEVQKYMTESNHGIGNYLLITNTEFWNGLPADIRTQLEAIVDEVTVEVNQRAEA
                     LNAEARQAIVDSGKSEIIALTPEQRASWRDAVSPAWKAFEADIGADVIEAAQAANQ"
     gene            complement(7248..8168)
                     /locus_tag="VFF22_17130"
     CDS             complement(7248..8168)
                     /locus_tag="VFF22_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX18578.1"
                     /translation="MRRIDFITLKLFVAIADERSLTRAAEREHLALAAVSKRISDLES
                     QLGIALLYRQPKGVELTPAGHALLHHARNMMDNLEQLNADLSEFSEGVKGHVRIHANT
                     SAVIEFLPEDLASFSRLHPQVKIDLEERVSSETIRAVREGLTDIGIFAGHVPADDLQV
                     FTYRHDQLVLVTPRDHPLAGREGIHLREAVGYDFIGLQQDASLHSLLQHSAHSQGVAL
                     RLRIQVRSFEAICRMIHTGMGVGVLPDLAVRTYLPALDVRVIPLHDPWARRELKVAVR
                     NLDNLSLTAKQLLEHLCNSASADKGFARPS"
     gene            8348..9550
                     /locus_tag="VFF22_17135"
     CDS             8348..9550
                     /locus_tag="VFF22_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZX18579.1"
                     /translation="MTQTSTGNMALQGLKVIEMGQLIAGPFASKLLGEFGADVIKIEP
                     PGVGDPLRKWRKIKDGTSLWWHVQSRNKRSLTLNLKEPEAQDIVRQLVAEADVLVENF
                     RPGTLEGWGLGYEALKAINPRLIMLRISGYGQTGPYRDLPGFGVIGEAMGGLRHLSGY
                     PGQAPVRVGISIGDSLSSLYGVIGVLLALQERARSGEGQEIDVALYESVFAMMESLVP
                     EYDAFGYIREPAGSALPGITPSNSYPCKDGSYVLIAGNGDSIYKRLMSLIGRDDLGND
                     PRLAQNDGRSEHAELIDSAIAEWTAQRGRDDVIEALKGARVPAGYPYTAADIVQDPHY
                     LARQMIEQVQTSAGPLKVPGVLPKLSRTPGRIGTGGPQLGEHNTDILAGLGLSEEQVA
                     GLRERGVI"
     gene            9722..10645
                     /locus_tag="VFF22_17140"
     CDS             9722..10645
                     /locus_tag="VFF22_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003289409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HZX18580.1"
                     /translation="MSKRLYIQDVATRDGFQIEASFVPTEDKIVLIDRLSHTGLAKIE
                     VTSFTSPKAIPNLRDAEEVMRDIQRVPGVEYTVLVPNVKGCERALSCEVDEINLVMSA
                     SDTHGLANLRMTPEQSLLQFREIIEVSRGSGVFINASLSTTFGCPFKGEVADSRVYEL
                     IQRLLDIGVQGVTLCDTTGMADPAQVERICREALHRWPEAVFTAHFHNTRGMGLANAL
                     AALNAGIERFDASLGGLGGCPYAPGASGNICTEDLVHMFQRMGLDTGVDLDALLDAAA
                     TLPGLIGHDVPGAILKAGKSDRRYPKPKWMV"
     gene            complement(10837..11736)
                     /locus_tag="VFF22_17145"
     CDS             complement(10837..11736)
                     /locus_tag="VFF22_17145"
                     /inference="COORDINATES: protein motif:HMM:NF027432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4824 family protein"
                     /protein_id="HZX18581.1"
                     /translation="MNGVRAHYLIAGLALIGLVNAIVLAGVAWNRQEPVESRLQLSER
                     ELAATYAYWRKDNSSLALRLDYRWPSRADDDNHYLSISAEKMAELGFQVPTELTEQTV
                     RRYRRQLDRDALLVLELDGPAYQREVALLVAAHAEALRLQKSVPDSKELREAAQHAAN
                     ALNYEQRRASRLLVVDVGLDQQALRARYPDRARYAIVRSIIEVQASSVATDWDGEGDD
                     PRPDNQRWTWQLGGSADTPGVQSLNLPQRWHATFDSLPQQNEQAGVDYSSYQKLFSAE
                     VAFGRRLEPWFVEFTGPVTTKVQ"
     gene            complement(11733..12806)
                     /locus_tag="VFF22_17150"
     CDS             complement(11733..12806)
                     /locus_tag="VFF22_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017519442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HZX18582.1"
                     /translation="MPNINRSDAQQRADDIGVFNRELARLEAAQVLNLSDSQRANLHG
                     YQQQLLNSYLAAFDIDHDIQARQLSLGMRIASFLGALALAASVFFLFYQFWGLFGTQS
                     QVAILLTASIASFGLTLWVQRLDSSGYFSKLAALVAFTCFVLNIVMLGQIFNITPSNN
                     ALLPWALYALLLAYACGARLLLAAGLSCLLAFVAARIGSWDGIYWLGFAQRPENFILP
                     ALLIFAVPLLIDQRRFDGFAVIYRVFGLLGVFLPVLVLSHWGAGSYLDYSDEYIENAY
                     QIGGFILAALVIWLGVRREWADVVNTGLSFFVIFLYTKLFDWWWESMPKYLFFLVLGL
                     SAVLILLVLRRLRSRHGLLGGAQ"
     gene            complement(12932..14485)
                     /locus_tag="VFF22_17155"
     CDS             complement(12932..14485)
                     /locus_tag="VFF22_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HZX18583.1"
                     /translation="MRIKVHCQNRIGILRDILNLLVEYGINVARGEVGGEQGNAIYLH
                     CPNLINLQFQSLRPKFEAISGVFGVKRVGLMPSERRHLELNALLGALDFPVLSIDMGG
                     SIVAANRSAAQLLGVRVDEVPGIPLSRYAEDFDLPELVRANKSRINGLRVKVKGDVFL
                     ADIAPLQTEHEESDAMAGAVLTLHRADRVGERIYNVRKQELRGFDSIFQSSKVMAAVV
                     REARRMAPLDAPLLIEGETGTGKELLARACHLASPRGQSPFMALNCAGLPESMAETEL
                     FGYGPGAFEGARPEGKLGLLELTAGGTLFLDGVGEMSPRLQAKLLRFLQDGCFRRVGS
                     DEEVYLDVRVICATQVDLSELCAKGEFRQDLYHRLNVLSLHIPPLRECLDGLPPLVEH
                     FLDQASRQIGCPLPKLAPQVLDKLGHYHWPGNVRQLENVLFQAVSLCDGGVVKGEHIR
                     LPDYGAPQPLGEFSVEGGLDEILGRFEKAVLERLFHDHPSSRQLGKRLGVSHTTIANK
                     LRQHGLGKE"
     gene            14745..15530
                     /gene="phhA"
                     /locus_tag="VFF22_17160"
     CDS             14745..15530
                     /gene="phhA"
                     /locus_tag="VFF22_17160"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HZX18584.1"
                     /translation="MKSTQYLAREPDDSGFIDYPEAEHQVWNTLITRQLKVIEGRACQ
                     EYLDGIEQLGLPHERIPQLGEINQVLQATTGWRVARVPALIPFQTFFELLASKQFPVA
                     TFIRTPEELDYLQEPDIFHEIFGHCPLLTNPWFAEFTHTYGKLGLAASKEERVYLARL
                     YWLTIEFGLVDTSQGQRIYGGGILSSPKETLYCLSDAPQHLAFDPIEAMRTPYRIDIL
                     QPLYFVLPELKRLFELAHEDIMAMVQQAMRLGLHAPKFPPKAA"
     gene            15591..15944
                     /locus_tag="VFF22_17165"
     CDS             15591..15944
                     /locus_tag="VFF22_17165"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HZX18585.1"
                     /translation="MSLAQAQCEACRADAPKVSDEELAELIREIPDWNIEVRGDHMEL
                     ERVYLFKNFRHALAFTNAVGAIAEEVGHHPALLTEWGKVTVTWWSHEMRGLHRNDFIM
                     AARTDVLAASAEGRK"
     gene            15941..17137
                     /locus_tag="VFF22_17170"
     CDS             15941..17137
                     /locus_tag="VFF22_17170"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HZX18586.1"
                     /translation="MSHFHNIVRVPGDPILGLMDAYRADRNPAKLDLGVGVYKDAQGL
                     TPIPRAVKLAEQRLVESETTKSYIGGHGDPLFGNLLSQLVLGADSPLLAERAGTTQTP
                     GGTGALRLCADFIAHCLPGRGIWLSDPTWPIHESIFAAAGLRVGHYPYVGADNQLNVE
                     AMIAALAHVPKGDVVLLHACCHNPTGFDLTPRDWKRVLEVVKARELLPLIDFAYQGFG
                     DGLEQDAFAVRLFAAELPELLITSSCSKNFGLYRERTGALIVCAETATKLQDVRSQLA
                     FIARNLWSTPPAHGAAVVASILADSELHNLWVSELDAMRQRVASLRAGLVEALQPYGL
                     SERFAHIALQRGMFSYTGLSPTQVQRLRDEFSVYMVGSGRANMAGLDAERLDQLASAI
                     SRVCCD"
     gene            17238..>18362
                     /locus_tag="VFF22_17175"
     CDS             17238..>18362
                     /locus_tag="VFF22_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HZX18587.1"
                     /translation="MPRLLNFAFRLYQRILHSLALYPTLIALGFFVLCLLSMSIEYQP
                     WMMSLKGEFDVGLVRNADNARLILGTLVGGILSLMVFSFSMFMVVLNNAAAALSPRVV
                     PGLISDKSHQKTLGFYLGTILFSLLLITTIELDNSTQVPSLGVLMALVMGIACLGLFV
                     HFIHSISRSIQVEYILNNLYHTTLAKLAAREAKMAAIDNLPSWPEEQNWQEVCAQRSG
                     YFKELNVNTINTILCEHDLRMTVMIHRGFFVMSGRPLFKLDREVDSAITDALLDCFDF
                     FLEEYAHTHYLFGCTQMSEIAVKALSPGINDPGTAITAIDLLSVLFSKRLALPDLDVT
                     LADDGPPRLLHYELSLDELLQRVFGPIRHYGCADVSVLIGL"
BASE COUNT         3758 a         5777 c         5425 g         3402 t
ORIGIN      
        1 acacgccctt caatacttcg tggctgccgt agcgcttgtg cagatcctga acttcaagtt
       61 tgtacatggt ttccactctc ttgaatcagt cgctgagcaa gcgaccctgg cgaatagggg
      121 tacgaccggc cactttagcc aaccacaaac cgggctgagc aaaacgcaac cgctcgcgcg
      181 catagagcac accatctgta acggcgcaga cagttgtgtg gcgatcatcc agaggattga
      241 tcacttcaaa caatggatcg ccggccttca cccgcgcgcc caccggctgc aaaaaactta
      301 ccaccccggc atggggggca taggcgtatt gcgcgccgtt aaacggcgtg gcatcgcagc
      361 tttcagcggg cgctggcggc cagtcaccgg caataaggcc ctgttcggca aggaacgcca
      421 gaatgctttc ggcactggct tcggcctcgg gtttgccggt atcggccatg ccgcccagtt
      481 cgactgttac tgccaggcag gccaacggca cagcagcctg ggggaaatgc cgggccagat
      541 gcacccaggg caaggcacag gcttcatcga aagaactgcc gccggaatcc tcgacagtga
      601 gcgccgcacc ggcctgcagg cgtgcagcaa gggagcgcaa ttgcggccac tgctgcggca
      661 gcaggtacag atgcactgca gcatcgaaat cacaatgcag gtcgagcacc acatcggcat
      721 cgcaggcatg tttgagcagc aggcgttgca tgccttgcag ctccgagccg gctggcggca
      781 aggcttccag cgcggcctgc atggcagcgc gaatcagccg cacattgctc gggccgtcac
      841 tgcccagaca gccttcaagc gcaggcgcga tggcggcggc cagatcaaag aaatcacggt
      901 tgaagttctt cccgctgccc atctcaaaac ggccctgatg ggtcgcctgg aacatctgcc
      961 ccaggccaat cgggttggct accggcacca gctcgatcac cccagtcagg cgcccctggg
     1021 cttccagctg acgcagacgg cgcttgagtt ccaccgccac gcgcatccca ggcagctcat
     1081 cggcatgcag cgaggcctgg atatacgcct tgcacggacc ctggccgaaa cggaacacgc
     1141 tcaagcggcg ttcggttcct gggcagcccc agggcagtgg atgatcgata cgctgcatgc
     1201 tctattcctc aggccttgcg cggggccaaa taggccagcc aacggcgttc ggccagcttg
     1261 aacaggcgta cgaggataaa ggtcaggcac aggtagaaca gcccggccgt gataaacgct
     1321 tcgaacggca tgtagtactg cgagctgacg gtacgcgccg cgccggtgat atcgatcagg
     1381 gtgactatcg aggccagcga ggtggtgtgc agcatcatga tcacttcgtt gctgtactgc
     1441 ggcagggcgc ggcgcagcgc cgacggcagc aggatgcggc ggtacagttt gaagcgcgac
     1501 atgcccatgg ccttggctgc ctcgatctcg ccatggggcg tggccttgag gctgccggcg
     1561 agaatctccg cgctgtaggc gctggtattg atggcaaacg caaggcaggc gcagaaggtg
     1621 gcgctggaga aatacggcca caacacgctc tcacgcaccg cctcgaactg cgccaggccg
     1681 tagtagatca gaaacagctg caccagcatc ggtgtgccgc gaattacgta ggtgtagagc
     1741 caggccggaa agttgatcag aggcgatttg gagacgcgca tcaaccccag gggaatggcc
     1801 agagccaggc cgcacgccag ggaaatcagc agaattttca gggtgaccag tacaccacca
     1861 aagtacagcg gcaggttttc caggattacg ttgtagtcaa aaatcatcgc tgcgctcccc
     1921 gcttacagat cagcggcttt gatgccgacc gagtaacgtt tttccaggta acgcaaagcc
     1981 agcagcgaca cgctggtcag caccagatac agggccgcaa cggctaggta gaaggtgaat
     2041 ggctcacggg tggcatcggc ggcacttttc gccttgaaca tcatgtcctg cagaccaacc
     2101 acagaaatca gcgcggtagc cttggtcaac accaaccagt tgttggtgaa cccaggaatg
     2161 gcgaagcgaa tcatctgcgg caccaggatg cgaaagaaca cctgcatgcc attcatgcca
     2221 taagccgcac cggcttcagc ctgacctttg gggatggcca taaaggcccc acggaaggtc
     2281 tccgacaggt aagcaccaaa gataaagccc atggtgaaca cgccggcaat aaaggggttg
     2341 atatcgatgt agtcgtcata gccgagcatc ggcgccagac ggttcaccat gtcctgaccg
     2401 ccgtagaaaa tcagcaggat cagcaccagg tcgggaatcc cgcggattac cgtggcgtag
     2461 gtttcaccca gcagcgccag ccacttgatt ggtgacaggc gaaacgccgc acccagcaaa
     2521 cccaacacga tggccactgc catcgacgtc agcgccagaa tcagggtgag ccaggcaccc
     2581 tctagaatgg tcgagccgta gccgttgagc atgctctgct tacctcatgc aaacgccggg
     2641 cggcgcctgg gagtacaacg ggaaagtggc gcaagcttca ggttatagcc agcttgcgcc
     2701 actttcagtg aaagcgactg agcgaactca atcagtcgcc gtagacgttg aagtcgaagt
     2761 acttgtcctg cacttccttg tacttaccgt tggcgcggat ggccaggatg gcagcgctga
     2821 tcttgtcagc cagggcctta tcgcccttgc gcacggcaat accggcgccc atgccgatgt
     2881 atttctcatc ggtgtagtca gggcctacca gggcgaagcc tttaccggcg tcggtcttga
     2941 taaagccatc gtcgatattg acgacgtcag caaaggtggc gtccagacga ccggaagtca
     3001 gatcgagaaa ggcttcgttc tgcgagctgt aacgcacgat ctcgataccg gcaggggcaa
     3061 agatatcggt ggcgtaacgg tcgtaggtgg atgcgcgctg tacgccgact ttcttgcctt
     3121 tcagatcaac cagcgggtcg ttgagtacag tacccgcttt catcgccagc ttgcccgggg
     3181 tctggtagta cttgccgctg aaatcaaccg atttcaagcg atcttcggtg atgctcatcg
     3241 aagacagtac ggcgtcgaac ttgcgcactt tcagggccgg gatcaggccg tcgaattcct
     3301 gctcgatcca tttgcattcc actttcatct cttcgcatag ggcgacgccg atgtcgtagt
     3361 cgaaaccgct gatggcacct tcaggggttt tgaaggcgaa cggcgggtaa gccgcttcga
     3421 taccgatacg cagaggcttg gcctcttcag ccatggccag cggcgacagc atggacagcg
     3481 ccagtgcgcc gagaagtgca atcttcttca ttttgtgact ccttcgaatg ggaatggcat
     3541 cggttggcag agattgttcg ggtatgcatc cagctttgca tgaattgtag tgagtcaccg
     3601 tcaagctgcg cagaagccag acggccccag ccgggtgagc ggcattctag cgacagctcg
     3661 aaagtcgata tttcttcaat gcgacaagta attacaaaag gcccggacgg gcatagcgag
     3721 gatattgaca gcccctggga tttatgcaga gacaaaaaag acagttaacc atttattaaa
     3781 gcaattaacg cgcctacttt taatcaggcc tttatattcg cggcctggtg gcatttttta
     3841 ttttcacagc cgcccagaac aatgcgccat cctggcgcaa aacgttcccg cacaccccat
     3901 aaaagtgcgc gttgttacgc ccaagtgaca agcaaatacc cagatgaaaa tcgataacgc
     3961 tacacaaagc acaacgccct gcccggcttg tggccgaaac agggcgttgc ttacaggcgg
     4021 tttaaccgaa gtcaggcaac ctgcatctcg cggtgggtgt cgataaggtg ctgcaccacg
     4081 ccaggatcgg ccaacgtgga gatgtcgccg agtgcatcgt attcggcggt agcgatcttg
     4141 cgcaggatac ggcgcatgat tttgcccgag cgggttttcg gcagaccagg ggcccactga
     4201 atcacgtccg gtgaggcaat cggaccgatt tccttgcgca cccagttctt caactcctgg
     4261 cgcaattgct cggaaggctg ctcgccacca ttcaaggtga cgtagacata gatgccctgc
     4321 cctttgatgt cgtgcggcac gccaaccacg gcggcctcgg cgactttcgg gtgagcaacc
     4381 atggcgcttt cgatctcggc tgtgcccata cggtggcccg acacgttcaa cacgtcgtca
     4441 acgcggccgg taatccagta gtaaccgtcc tcatcacggc gcgcgccgtc accggtgaag
     4501 tacatgccct tgaaggtctt gaaataggta tcgacgaaac ggtcatggtc gccgtaaaga
     4561 gtccgcgcct gacccggcca ggaatcgatg atcaccaggt tgccttcggc agcgccctcg
     4621 accaggttgc caaggttatc caccagagca ggctgcacgc cgaagaacgg acgagtcgcc
     4681 gagcctggct tgagcgcggt agcgcccggc agcgggctga tcaggatgcc gccagtttcg
     4741 gtctgccacc aggtatcgac aatcgggcaa cgctctttgc cgacggcctt gtagtaccag
     4801 ttccaggctt ccgggttgat cggctcacca accgagccga gcagacgcag gctggagcca
     4861 tcggcaccag ctaccgccgc atcgccatga gccatcatgg cgcggatagc agtcggtgcg
     4921 gtgtagagga tattgacctt gtgtttgtcg atgatcttcg acacccgggt gatatccggg
     4981 tagttcggga tgccttcgaa cagcagcgta gtcgcaccat tggccagcgg gccgtagacg
     5041 atatagctgt ggccggtaac ccagccgacg tcggcggtgc accagtagat atcgcccggc
     5101 ttgtagtcga acacccgctc atgggtcagc gccgcataca ccagataacc gccggtggtg
     5161 tgctgcacgc ctttcggctt accggtggag cccgaggtat agaggataaa cagcgcttcc
     5221 tcggcgccca tttccttcgg cgcgcaatgg ctggaagcca cggccatcag gtcttcgtac
     5281 cagatgtcgc gatgctggtt ccacttgatc gggctaccgg tgcgcttgca cacgataacc
     5341 ttctggatgc tcgcagtttc cgggttggtc agcgcgtcgt cgacgttggc tttcagcggg
     5401 gttttcttgc cgccacgcag gccttcatcg gcggtgatca ccaccttgga ctggcaatca
     5461 ataatgcgcc cggccagcgc ttccggggag aagccgccaa acaccaccga gtggatcgcg
     5521 ccgatacggg tacaggccag catcgctacc accgcctgcg gaatcatcgg catataaatg
     5581 gtcacgacat cgccgcggtg cacgtcctgg ccacgcaggg cgttagcgaa cttgcagact
     5641 tgttcgtgca gctggcggta ggtgatttcc tcgtgctcgg acgggtcatc gccttcccag
     5701 atgatcgcca cctgatcgcc acgctcttcc aggtgacggt ccaggcagtt gtaggaaacg
     5761 ttgagggtgc catcggcaaa ccatttgata tcgacgtggt gatcgtcgaa agaggtctgc
     5821 ttgaccttgg tgaacggctt gatccagtcc agccgctggg cctgttcgcg ccagaaacca
     5881 tcggggttaa tcaccgactg ctggtacatg gctttatagg tggcctcatc agtcagcgac
     5941 tgggcggcca cctcagggcg cacgggatac aaggacgcag cactcatcag aaacacctcg
     6001 gtgggtagtt gttgttctat gggccatttt gtagccagga ccccaccttt gagccattcg
     6061 accatggtct taccgctcta cgaccatggt cttggagggc tctatttgcc tgcaaacacc
     6121 caggcacttt tattgattgg ctgcctgagc cgcttcaatc acatcagcgc cgatatcggc
     6181 ctcgaaggcc ttccaggctg ggctcacagc atcacgccag gaggcacgct gctcaggcgt
     6241 cagggcgatg atctcgctct tgccggagtc gacgatggcc tggcgagctt cggcgttgag
     6301 cgcttcggcg cgctggttca cctcaacggt gacctcatcg acgatggctt ccaactgcgt
     6361 gcgaatatcc gcaggcaaac cgttccagaa ttcggtgttg gtgatcagca ggtaattgcc
     6421 gatgccatgg ttggactcgg tcatgtactt ctgcacctcg aagtacttct gcgagtagat
     6481 gttcgaccaa gggttatcct gggcgttgac tacacccgtc tgcaggccct ggtagatctc
     6541 ggcgaagctc atcttgcgcg gcaccgcgcg cagggcgctg tactgcgcgg cttgcaggtc
     6601 ggacggctgc acgcggaact tctgcccgcg ggcatcagcc ggctcgcgca ggggcttgtt
     6661 ggcggtgagc tgacgcatgc cgttgagcca ataggccagg ccggtgatgc ccttgctctc
     6721 catcgatttg agcagcgcct tgcctttatc cgatgcctgg aagcgctggg cagcagccac
     6781 gtcgtcgaac agttgcagct gcttggtgta ctgctccagt ttggagggtg ccggtgccag
     6841 catctgcacc tcattgagca gcagggcttc catctccttg ccgtcgccat acagcgagga
     6901 gttggcgtac acatcgacct tgaccttgcc ggccaggcgc tcttccacca gctttttgaa
     6961 cagcagggcg ccctggccct tgggcgtggt gtcggcggtg atatgggaga atttaatcag
     7021 aataggggcg gtcggctgcg actcggcggc ctgggccgat aacgcaccga tcaaggcaca
     7081 accggcaacg atgccgacaa gaccgcggcg cacaaatgaa gacgtcatgg aggagctcct
     7141 gttgtttttg tctgagcctc gggtactgga tagccccgat tggccggact ctactccgcc
     7201 ctccccgctg cctgacattc atatttggac aagcacgcct tcggctttca cgaaggccgc
     7261 gcgaaaccct tatctgccga cgcgctattg cacaggtgct cgagcaactg cttcgccgtc
     7321 agcgaaaggt tgtccaggtt gcgcaccgcc actttcagct cgcgacgcgc ccagggatcg
     7381 tgcagtggga tcacccgtac atccagggct ggcagatagg tgcgcaccgc caggtccggc
     7441 aacacgccga cgcccatgcc ggtatggatc atccggcaga tggcctcgaa gctgcgcacc
     7501 tggatacgca ggcgcaaggc cacaccctgg ctgtgcgccg aatgctgcag caggctgtgc
     7561 agcgaggcgt cctgctgcag gccgataaaa tcgtagccca ccgcttcacg cagatggatg
     7621 ccctcgcgcc cagccagcgg gtggtcgcgc ggcgtcacca gcaccaactg gtcgtgccga
     7681 taagtgaata cctgcagatc atcggccggt acatgaccgg cgaaaatacc aatatcggtc
     7741 agtccttcgc ggacggcacg gatggtctcg ctgctcaccc gctcctccag gtcgatcttc
     7801 acctgcgggt gcaggcggct gaagctggcc agatcttccg gcagaaactc gatcaccgcc
     7861 gaggtgttgg cgtgaatgcg cacatggccc ttgacccctt cgctgaactc gctgagatcg
     7921 gcgttgagct gctcaaggtt gtccatcatg ttgcgcgcat ggtgcagcag cgcgtggccg
     7981 gccggggtca gctcgacgcc tttgggctga cgatagagca aggcgatgcc cagctggctc
     8041 tccaggtcgc tgatacgttt gctcaccgcg gccagggcca ggtgctcgcg ctcggcggcg
     8101 cgggtcaggc tgcgctcgtc ggcgatggcg acgaagagtt tgagggtgat gaagtcgata
     8161 cgacgcatgg gggctctccg gttcgtgcct gacgaagcag tgacaggatc tcactgcggc
     8221 tacggcgtcc taaagcatga cacttcgcca tacgcgaagc ctagcttggc gattgatcaa
     8281 ttgcccccac cccagcgccg ggccatcctg cccccacaaa aaacgcacag tcatatcggg
     8341 tgcagccatg actcaaacca gtacaggcaa tatggccctg caggggctca aggtgatcga
     8401 aatgggccag ctgatagccg gccccttcgc cagcaagctg ctcggcgaat tcggcgctga
     8461 cgtgatcaag atcgagccgc cgggcgtcgg cgatccgctg cgcaaatggc gcaagatcaa
     8521 ggacggcacc tcgctctggt ggcacgtgca atcgcgcaac aagcgctcgc tgaccctaaa
     8581 cctgaaagag cctgaagccc aggacatcgt ccgccaactt gtcgcagaag ccgacgtgct
     8641 ggtggagaac ttccgccccg gtacgctgga aggctggggc ctgggctatg aagcgctgaa
     8701 ggcgatcaat ccgcggctga tcatgctgcg catctccggc tacgggcaga ccggccccta
     8761 ccgcgacctg ccgggcttcg gcgtgattgg cgaggccatg ggcggcctgc gccacctctc
     8821 cggctacccc ggacaggcgc cggtgcgcgt cggtatcagc atcggcgatt cgctgtcgtc
     8881 gctgtacggc gtgatcggcg tgctgctagc cttgcaggaa cgggcgcgca gcggcgaagg
     8941 ccaggagatc gacgtggcgc tgtacgagtc ggtgttcgcc atgatggaaa gcctggtgcc
     9001 ggagtacgac gccttcggct atatccgcga gccggccggc agcgccttgc ccggcatcac
     9061 gccgtccaat tcctacccct gcaaggatgg cagctatgtg ctgatcgccg gcaatggcga
     9121 cagcatctac aaacgcctga tgagcctgat cggccgcgac gacctgggca atgatccgcg
     9181 cctggcgcag aacgacgggc gcagtgagca cgccgagctg atcgacagcg ccattgccga
     9241 atggaccgcc cagcgcggcc gcgatgatgt gatcgaagcg ctcaagggcg cccgcgtacc
     9301 ggctggctac ccctacacag ccgccgatat cgtgcaggac ccgcactacc tggcgcggca
     9361 gatgatcgag caggtgcaga ccagcgccgg cccgctcaag gtgccgggcg tactgcctaa
     9421 gctcagccgc acgccagggc gcatcggcac cggcggcccg caactgggcg aacacaacac
     9481 cgacatcctc gccggcctcg gcctgagcga agagcaagtc gctgggctgc gcgaacgtgg
     9541 cgtcatctag ccagatggct cccacgctcc gcgtgggagc aacgcccagg acgctctgtg
     9601 tccgcgccta acagcagcga cgcagagcgt cacccgatgc attcccacgc agagcgtggg
     9661 aacgatcaat caagccggtg cgcacagcgc accctacgcc gcgaaccacc aggatcacgc
     9721 catgagcaaa cgcctctaca tccaggacgt cgccacccgt gacggcttcc agatcgaagc
     9781 ttctttcgta cccaccgaag acaagatcgt cctgatcgac cgcctgtccc acaccggcct
     9841 ggccaagatc gaggtgacct ccttcacctc gcccaaggcc atccccaacc tgcgcgatgc
     9901 cgaagaggtg atgcgcgaca tccagcgcgt accaggcgtc gagtacacgg tgctggtgcc
     9961 caatgtgaag ggttgcgagc gcgcgctgtc gtgcgaggtg gacgagatca acctggtgat
    10021 gtccgccagc gacacccatg gcctggcaaa cctgcgtatg acgccggaac agtcgctgct
    10081 gcagttccgc gagattatcg aggtcagccg gggcagcggc gtgtttatca acgcctcgct
    10141 gtcgaccacc ttcggttgcc cgttcaaggg cgaggttgcg gacagccgcg tctacgagct
    10201 gatccagcgc ctgctggaca tcggcgtgca gggtgtaacc ctatgcgaca ccaccggcat
    10261 ggccgacccg gcccaggtcg aacgcatctg ccgcgaggcg ctgcatcgct ggcccgaggc
    10321 ggtgttcacc gcacatttcc acaacacccg cggcatgggc ctggccaacg ctttggcggc
    10381 gttgaatgcc ggcatcgagc gcttcgacgc ctccctcggc ggccttggtg gctgtcctta
    10441 cgcaccgggt gccagcggca atatctgcac cgaggacctg gtgcacatgt tccagcgcat
    10501 gggcctggac acaggcgtcg acctggatgc tctgctcgac gccgccgcca ccttgcccgg
    10561 cctgatcggc catgatgtgc cgggggccat cctcaaggca ggcaagtcgg atcgacgtta
    10621 tcccaagccg aagtggatgg tatagggagc gaatttattg gcgaaattag cggcaccatc
    10681 gctagtgcat cccgcggcac caaccggcag acgaaaaaaa gcgacccact gaggggtcgc
    10741 ttgctcgaac actcgtataa ccgagtagag aaacgcttgc atccaatcca cgcagggttg
    10801 aataacccca ctgtgcatcg ctgctaatgc gacgggctat tgcaccttgg tcgtaacagg
    10861 cccggtgaac tcgacaaacc agggctccag ccgccggccg aaggcgactt cggcgctaaa
    10921 cagcttctga taactcgagt aatcaacgcc tgcctgctcg ttctgctgcg gcaggctgtc
    10981 gaacgtcgca tgccagcgct gtggcagatt gaggctctgc accccagggg tatcagccga
    11041 gccgccaagc tgccaggtcc agcgctggtt gtccgggcgc gggtcgtcgc cctcgccgtc
    11101 ccaatcggtc gccacactgc tggcctgcac ctcgataatg ctgcgcacaa tggcgtagcg
    11161 cgcacggtcc gggtagcgcg cccgtaatgc ctgctgatcc aggccaacat ccaccaccag
    11221 caaacggctg gcacgccgtt gctcgtaatt cagtgcattc gcggcatgct gagccgcctc
    11281 gcgcagctct ttgctatccg gcaccgattt ctgcaaacgc agggcctcgg catgggcggc
    11341 aaccagcagc gcgacttcac gctgataagc cggaccatcg agttccaaca ccagcagtgc
    11401 atcacggtcc agctgacggc ggtaacgccg taccgtttgc tcggtcaact cagtgggcac
    11461 ctggaagccc agctcggcca tcttctcggc actgatcgac aggtagtgat tgtcatcatc
    11521 cgcgcggctc ggccagcggt aatccaggcg cagcgccagg ctgctgttgt ccttgcgcca
    11581 ataagcgtag gtcgcggcca gctcgcgctc ggacagttgc agacggcttt ccaccggctc
    11641 ctgacgattc caggccacac cggccaggac aatggcattg accaggccaa tcaacgccag
    11701 gccggcaatc agataatgcg cacgcacgcc gttcattgcg cacctcccag caagccgtgg
    11761 cggctacgca ggcgccgcaa taccagcaga atcagcaccg cactcaaacc cagcaccagg
    11821 aagaacaggt acttgggcat gctctcccac caccaatcga agagcttggt gtagaggaag
    11881 atcacaaaga agctcagccc ggtgttcacc acatccgccc actcgcggcg cacacccagc
    11941 cagatcacca gggctgcgag tataaagccg ccaatctggt aggcgttctc gatgtactcg
    12001 tccgagtaat ccaaatagct acccgcgccc caatgcgaca acaccagtac cggcagaaac
    12061 acgccgagca ggccaaatac ccgatagatc accgcaaagc cgtcaaaacg gcgctgatca
    12121 atcagcagcg gcaccgcgaa gatcagcaac gccggcagga tgaaattctc cggccgctgg
    12181 gcaaaaccca gccagtagat gccatcccag ctgccaatcc gcgccgcaac aaaggccagc
    12241 aaacaactca acccggccgc cagcagcaga cgcgccccgc aggcataagc cagcaacagc
    12301 gcatagagcg cccagggcag caaggcgttg ttcgacgggg tgatattgaa gatctgcccg
    12361 agcatgacga tgttcaggac aaagcaggta aaggccacca gcgccgccag cttgctgaag
    12421 tagcccgagc tatcgaggcg ttgcacccac agcgtcaaac cgaacgaggc gatgctcgcc
    12481 gttagcagaa tcgccacttg gctctgggtg ccgaacaaac cccagaactg gtagaacaga
    12541 aaaaacacac tggccgccaa cgccagcgcg ccgaggaacg aggcgatgcg catgcccagc
    12601 gacagctggc gggcctggat gtcgtgatcg atatcgaaag ccgccaaata gctgttgagt
    12661 aattgctgct ggtagccgtg caaattggcg cgctggctat cactcaggtt caacacctga
    12721 gccgcctcaa ggcgcgccag ttcgcggttg aaaacgccga tatcatcggc acgttgctgg
    12781 gcatcactgc ggtttatgtt gggcatggca atccttgctg cgggtccatc aggtcatagg
    12841 cggatcagca cactctagcg ctaaattttc ggcgataggc tgacccagta gagggttcgt
    12901 aactgcgaac aactcttgcg attaaagccc cctactcctt cccaaggcca tgctggcgca
    12961 gcttgttggc gatggtggtg tgcgacacac cgagacgctt gcccagctga cggctgctcg
    13021 ggtgatcgtg gaacaggcgc tccagcacgg ctttctcgaa gcgcccgaga atttcatcca
    13081 gcccgccttc cactgaaaat tcgcccagcg gctgcggcgc accgtagtcc ggtaggcgga
    13141 tatgttcgcc cttgaccacc ccgccgtcac acagtgaaac ggcctggaac agcacgttct
    13201 ccagttgacg cacgttgcct ggccagtggt agtggcccag tttatccagc acctgcggcg
    13261 ccagcttggg cagtgggcag ccgatctgtc ggctggcctg atcgagaaag tgctccacca
    13321 gcggcggcag gccatccagg cattcgcgca gcggcgggat atgcaggctg agaacgttga
    13381 gacggtgata aaggtcctgg cggaattcgc ccttggcgca cagctcagac aaatcgacct
    13441 gggtggcgca gatcacccgc acatccagat aaacctcctc gtcactgcca acgcggcgga
    13501 agcagccgtc ctgcagaaaa cgcagcagct tggcctgcag acgcgggctc atttcgccca
    13561 caccatcgag aaacaacgtg ccaccggcag tcagctccaa caggccgagc ttgccttccg
    13621 gccgcgcgcc ttcgaaagca ccggggccgt agccgaacag ctcagtttcg gccatggatt
    13681 ccggcaagcc ggcgcagttc agcgccataa acggcgactg cccacgcggg ctggccaagt
    13741 ggcaggcgcg ggccagcagc tccttgccgg tgccggtttc gccctcgatc agcagtggcg
    13801 catccagcgg cgccatacgc cgtgcttcgc gcaccacggc ggccatcacc ttggagctct
    13861 ggaagattga atcgaagccg cgcagctcct gcttgcgcac gttgtagatg cgctcaccga
    13921 cgcgatccgc gcggtgcaag gtcaacaccg cgccggccat ggcatcgctc tcttcatgct
    13981 cggtttgcag cggcgcgata tcggcgagaa atacatcgcc tttcactttc acccgcaggc
    14041 cgtttatccg cgacttgttg gcgcgcacca gttccggcaa gtcgaagtcc tcggcgtagc
    14101 gcgacagcgg gatacccggc acctcatcca cccgcacgcc caacagctgc gcagcgctgc
    14161 ggttggccgc gacgatggag ccgcccatat cgatcgacaa caccgggaaa tccagcgcac
    14221 cgagcaaggc attgagctcc agatggcggc gttcactggg catcagaccg acgcgcttaa
    14281 ccccgaacac cccggaaatc gcctcgaact tgggccgtaa cgactggaac tgcaggttga
    14341 tcaggttcgg gcagtgcagg tagatggcat tgccctgctc gccgccgact tcgccacggg
    14401 cgacgttgat gccgtattcg accagcaggt tgaggatgtc gcgcaggatg ccaattcggt
    14461 tctggcagtg gactttgata cgcatggcag ggctctgatc tggcttctta ttagggccgt
    14521 agcgcgaaag aatcggcgct gaaggcggat tattcgtcaa gaatatgtga cagattagcg
    14581 cagcaacaag acgatagttc gccataaagc cgcacacgta accatttctt tacgaaataa
    14641 cgcacctttc ctcccgtatc agaccccggc ctgggctgca taaccgcgcg ggctgacgta
    14701 ttcctgagtc aaataaaaca acaaagcccc agcaggaggc agcgatgaag agcacgcaat
    14761 acctggcgcg cgaaccggat gacagtggct ttatcgatta cccggaggct gagcatcagg
    14821 tgtggaatac cctgatcacc cgccagctca aggtgatcga agggcgcgcc tgccaggaat
    14881 acctggacgg tatcgaacaa ctcggcctgc cccacgagcg tattccgcag ctcggcgaga
    14941 tcaatcaggt gttgcaagcc actaccggct ggcgcgtggc gcgggtgccg gcgctgattc
    15001 cgtttcagac cttcttcgag ctgctagcca gcaagcaatt cccggtggcg acctttattc
    15061 gcacgcctga ggagctggac tacctgcagg agccggacat tttccacgag atctttggcc
    15121 actgcccgct gctgaccaac ccctggttcg ccgaattcac ccatacctac ggcaagcttg
    15181 gcctggccgc cagcaaggaa gagcgcgtat acctggcgcg gctgtactgg ctgaccatcg
    15241 agttcggcct ggtcgacacc agccagggcc agcgcatcta cggcggcggc atcctgtcct
    15301 cgccgaaaga aaccctctac tgcctgtccg acgcgccgca gcacctggcc ttcgacccga
    15361 ttgaagcgat gcgtacgccg tatcgcatcg acatcctgca gccgctgtat tttgttctac
    15421 cggagcttaa gcgcctgttc gagctggctc atgaagacat catggccatg gttcagcaag
    15481 ccatgcgtct aggcctgcat gcaccgaagt ttccgccgaa agcggcataa cctttgtagg
    15541 atggcccgac acccatccta cggctcactt ttgacccttg gagaatcccc atgtcccttg
    15601 cccaagccca atgcgaagcc tgtcgcgccg atgcgccaaa agtcagtgat gaagagttgg
    15661 ccgaactgat ccgcgagatc ccggactgga atatcgaagt acgcggcgat catatggagc
    15721 tggaacgggt ttacctgttc aagaacttcc gccatgccct ggcttttacc aacgcggtcg
    15781 gcgccatcgc cgaggaagtc ggccaccacc cggcactgct caccgaatgg ggcaaagtca
    15841 ccgtgacctg gtggagccat gaaatgcgcg gcctgcaccg caacgacttc atcatggccg
    15901 cacgcaccga tgtactcgcc gccagcgccg agggccgcaa gtgagccatt tccacaacat
    15961 tgtgcgagtg ccgggcgacc cgattctcgg cctgatggac gcctaccgcg ctgaccgcaa
    16021 cccggccaag ctggatctgg gcgtgggcgt gtacaaggac gctcagggcc tgacgccaat
    16081 tccacgcgcg gtaaagctcg ccgaacagcg gctggtggaa agcgaaacaa ctaagagcta
    16141 tatcggcggc cacggcgacc cactgtttgg caacctgctt agccagctgg tactgggcgc
    16201 cgacagcccg ctgctggccg aacgcgctgg caccacccag acgcctggcg gcaccggtgc
    16261 gctgcgcctg tgtgcggact ttatcgccca ctgcctgccg ggccgtggca tctggctgag
    16321 cgatccgacc tggccgatcc acgagagcat ctttgctgcc gccggcctgc gcgtcggcca
    16381 ttacccctat gtcggcgcgg acaaccagct gaatgtcgag gcgatgatcg ccgccctcgc
    16441 ccacgtgccc aaaggcgatg tggtgctgtt gcacgcctgc tgccacaacc ccaccggctt
    16501 cgacctcacc ccgcgtgact ggaagcgcgt gctggaggtg gtcaaagccc gcgagctgct
    16561 gccgctaatc gacttcgcct accaaggttt tggtgatgga ctggagcagg acgcctttgc
    16621 cgtgcgcctg tttgccgccg agttgcccga actactgatc accagctcct gctcgaagaa
    16681 tttcggtctg taccgcgaac gcaccggcgc gctgatcgtc tgcgccgaaa ctgcgacaaa
    16741 gctgcaggac gtgcgcagcc agctggcgtt tatcgcccgc aatctgtggt ccactccgcc
    16801 ggcccacggc gcggcagtgg tcgccagcat tctggctgac agtgaactgc acaacctctg
    16861 ggtcagcgaa ctggacgcca tgcgccagcg cgtcgccagc ctacgcgccg gcctggttga
    16921 agccctgcaa ccgtatggtc tgagtgagcg ctttgcgcat atcgcgctgc agcgcgggat
    16981 gttctcctac accggcctga gcccaaccca ggtgcaacgc ctgcgcgatg agttcagcgt
    17041 ctatatggtc ggttcaggcc gggcgaatat ggccgggctc gacgccgagc gcctggacca
    17101 gctggccagc gccatttcgc gggtctgctg cgactgaaac gcacctgagc cccgcagcgg
    17161 ctacgccggg gctcaggtag cagcatgtgc cgacaagctt gctatagtcg ccgcacgccc
    17221 atcgcaccga gacgcccgtg ccccgcttgt tgaatttcgc cttccgcctg taccagcgca
    17281 tcttgcacag cctggccctc tatccaacgc tgatcgcgtt gggcttcttc gttttgtgcc
    17341 tgctgagcat gagcatcgaa taccagccct ggatgatgtc gctcaaaggc gagtttgatg
    17401 tcggcctggt gcgcaatgcc gacaatgccc gcctgattct cggcacgctg gtcggcggca
    17461 tcctgtcgct gatggtgttc agcttctcca tgttcatggt ggtgctcaac aatgccgccg
    17521 ccgcgctgtc accgcgcgta gtgcctggcc tgatcagcga taaaagccac cagaagacct
    17581 tgggtttcta cctcggcacc atcctctttt cgctgctgct gatcaccacg attgaactgg
    17641 acaactccac ccaggtgccc agcctcggcg tattgatggc gttggtgatg ggtatcgcct
    17701 gcctcggcct gtttgtgcac tttatccatt cgatttcccg ctcgattcag gtggagtaca
    17761 tcctcaacaa tctgtaccac accaccctgg cgaaactcgc cgcgcgcgaa gccaaaatgg
    17821 ccgctatcga caacctgccc agttggccag aggagcaaaa ctggcaggag gtctgcgcgc
    17881 aacgcagcgg ttatttcaaa gagctcaacg tcaacacgat caacaccatc ttgtgtgagc
    17941 acgacctgcg catgaccgtg atgatccacc gcggcttctt cgtgatgagt ggccggccgc
    18001 tgttcaagct tgaccgtgag gtcgattcgg caatcaccga tgcgctgctc gactgcttcg
    18061 atttttttct cgaggaatac gcccacaccc actacctgtt tggctgcacg cagatgtcgg
    18121 aaatcgccgt caaggcgctc agccccggca tcaacgaccc cggcacggcg attaccgcca
    18181 tcgacctgct cagcgtgttg ttcagcaagc gcctggcgtt gccggacctg gatgtcaccc
    18241 tggcggacga tggcccgcca cgcctgctgc attacgaact ctcgctggat gagttactgc
    18301 aacgggtatt cggccccatt cgccactacg gctgcgccga tgtgagtgtg ctgatcggtt
    18361 tg
//
LOCUS       DASRUM010000255        43020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_301494, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000255 DASRUM010000000
VERSION     DASRUM010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 43020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43020
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301494"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1033
                     /locus_tag="VFF22_17180"
     CDS             62..1033
                     /locus_tag="VFF22_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016985762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HZX18588.1"
                     /translation="MKLKRLMAAMTFVAAGVATATAVAAVDPALPSYTKTSGVSGNLS
                     SVGSDSLANLMTLWAEEFKKEYPNVNIQIQAAGSSTAPPALTEGTASMGPMSRPMKDA
                     EIQAFEEKYGYKPTAVPVAIDALAVFVHKDNPIKSLDMAQVDAIFSSTRLCGGATDIK
                     TWGDLGMTGEWAAKPIQLFGRNSVSGTYGYFKEEALCKGDYKANVNEQPGSASVVQSI
                     SSTINAIGYSGIGYKTSSVKTVPLSKKGGEAFDANEENALAGKFPLARFFYVYVNKAP
                     NKPLSPLDAEFVKLVLSKQGQEVVVKDGYIPLPSKVAAKTLKELGLE"
     gene            1214..3499
                     /locus_tag="VFF22_17185"
     CDS             1214..3499
                     /locus_tag="VFF22_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease"
                     /protein_id="HZX18589.1"
                     /translation="MNDLASETMTATSKSLGLDFNTPALLRKRRIRALKDRFARWAVS
                     IGGMAVLGAITLIFFYLAYVVLPMFQGASMDSRKPVQVAWLNEAAAPLLLTLEEQNQM
                     GMRLDQNGQVQFFDAKKMQPLSSVQLPIPADVRIASVGQDQPGSNRVALGLSNGQVLV
                     FEHVYKLSYPNDVQTITPSIEYPFGEAPIELDPQGRELDHMGVSLNSGTLMLAGSTGT
                     ELHVLSLGREENMMTGEVTLSEERVALPQIAEPIKALIIDPRHLWLYVVNGRATADVF
                     DMRSNSLNGRYKLLEDGKLEVSNATSLLGGISLMIGDTSGGIAQWFMVREADGKAVLK
                     SIRSFQLADSPIIQILPEERRKGFFALDAKGNLGIFHSTAHRTLLVEPVADGQALATL
                     SPRANRVLVESDGQLQRLIIDNPHPEISWSSLWGKVWYENYDAPGYVWQSTSASTDNE
                     PKLSLAPLAFGTLKAAFYAMLLAAPLAIAAALYTAYFMAPALRRKIKPVIELMEALPT
                     VILGFFAGLFLAPYVEGHLPGIFSLLIFTPIGILLAGYLWSRLPESIRLSVPDGWESA
                     MLIPVILLVGYVSLAMSGHVENWMFDGNMRMWLSNDLGIPFDQRNALVVGLAMGFAVI
                     PTIYSIAEDAVFSVPKSLTFGSLALGATPWQTLTRVVILTASPGIFSAVMIGMGRAVG
                     ETMIVLMATGNTAIMDMNIFEGLRTLAANVAVEMPESEVGGTHYRVLFLSALVLLGFT
                     FVMNTLAELVRQRLRVKYASL"
     gene            3515..5191
                     /gene="pstA"
                     /locus_tag="VFF22_17190"
     CDS             3515..5191
                     /gene="pstA"
                     /locus_tag="VFF22_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZX18590.1"
                     /translation="MSVKQNNLKTWFKSGTPWIWMNAGAVSIAVIMTLGLMMVIAVRG
                     LGHFWPADIVEADYQIPGQQAKVMAGEVTQIEEVPRARLAASGLPVEAEGGEFMTREL
                     FKVGNRDVYGADFTWVVGEWLSNLRTPENLTALERREWGNFYGYLVNVKENGELVAEG
                     DASWPALQERLERVEQLHKNLVRLEKKDIGRINAGLERIRLQTRKLELQGKLDDVAQA
                     ELDAERAQWDAEYKVLETELIALNQAFNRDSVTLKTADGREVTISLGKVVRAFQPNAM
                     GMGDKLGFYFAKLWEFVSDDPREANTEGGIFPAIFGTVMMTLIMAVIVTPFGVVAAIY
                     LREYAKQGPLTRVIRIAVNNLAGVPAIVYGVFGLGFFVYVLGGSLDRMFFPEAAPAPT
                     FGTPGLLWASLTLAILTLPVVIVATEEGLARIPRAVREGSLALGATKVETLWRVVLPM
                     ASPAMMTGLILAVARAAGEVAPLMLVGVVKLAPSLPLDGNYPYLHLDQKIMHLGFHIY
                     DVGFQSPNVEAARPLVYATALLLVLVIVVLNMAAVYIRNHLREKYKALDH"
     gene            5255..6088
                     /gene="pstB"
                     /locus_tag="VFF22_17195"
     CDS             5255..6088
                     /gene="pstB"
                     /locus_tag="VFF22_17195"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZX18591.1"
                     /translation="MQHETHTHGIDMAALGRDRQGLNMANERVAIDVPGLNLYYGEKQ
                     ALFDVKMTIPNQRVTAFIGPSGCGKSTLLRTFNRMNDLVDGCRVEGEINLDGKNIYRK
                     GEDVAELRRRVGMVFQKPNPFPKSIYENVVYGLRIQGINQKRVLDEAVEWALKSAALW
                     DEVKDRLHESALGLSGGQQQRLVIARTVAVQPEVLLLDEPCSALDPISTLKVEELIYE
                     LKSKYTIVIVTHNMQQAARVSDYTAFMYMGKLIEFGDTDTLFTNPAKKQTEDYITGRY
                     G"
     gene            6142..6873
                     /gene="phoU"
                     /locus_tag="VFF22_17200"
     CDS             6142..6873
                     /gene="phoU"
                     /locus_tag="VFF22_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZX18592.1"
                     /translation="MINKENLTQHISQQFNVELEEVRSHLLAMGGLVEKQVNDAVTAL
                     IDADSGLAQQVREIDDQINQMERNIDEECVRILARRQPAASDLRLIISISKSVIDLER
                     IGDEATKIAKRAILLCEEGEAPRGYVEVRHIGDQVRKMVQEALDAFARFDADLALSVA
                     QYDKTVDREYKTALRELVTFMMEDPRSISRVLNIIWALRSLERIGDHARNIAELVIYL
                     VRGTDVKHIGLTRMQEEVQGTKKGN"
     gene            7019..7933
                     /locus_tag="VFF22_17205"
     CDS             7019..7933
                     /locus_tag="VFF22_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019407560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX18593.1"
                     /translation="MSKVSVLVVDDATFIRDLVKKGLRDHFPGIQIEEAVNGRKALQM
                     LTRQPVDLILCDWEMPEMSGLELLTWCREQEALKIVPFIMVTSRGDKENVVQAIQAGV
                     SDFIGKPFSNEQLITKVKKALSRAGKLQALMSSAPPKMLSSGSFANDSLSALTGGKAD
                     VVKPTAGPAVQAAAAFAGKPAAAKVPAASATSGRGQGQLRLPGGATMSCVIKALSLKE
                     ALLVVKRTEVLPQVLESAVLDLEQGEAAETARLNGYLHAVAAFEPKPDSEWLQLTFRF
                     VDRDPQKLDYISRLIARGTAQKHFSPGA"
     gene            8059..8955
                     /locus_tag="VFF22_17210"
     CDS             8059..8955
                     /locus_tag="VFF22_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003297917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZX18594.1"
                     /translation="MLGRLILFCSLLTLAGQAAALTIYKYTDANGVVTYTDQAAPGAQ
                     VFVFRDRMVERLDNQVKLETKKHEAGETLLVRNDLHAPVQIELRLEATDNVTGVPDKP
                     ISWVLPPRSKIRLATLAPRDASKPMRYTPKLRYAMGDPRLLPTQKSYPLPWRGGPFRL
                     TQGANGKYSHFTPKGRYAMDIAMPEGTPIVAARGGIVVKTENQQSGRGNNPSGNYVRI
                     LHDDGTMGVYLHLMKGSVAVSEGARINAGSMLARSGNTGNSTGPHLHFVVQRNVGLAL
                     ESIPFNFAQPVNSLPNFAVGGE"
     gene            9044..9450
                     /locus_tag="VFF22_17215"
                     /pseudo
     CDS             9044..9450
                     /locus_tag="VFF22_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
     gene            complement(9588..10331)
                     /locus_tag="VFF22_17220"
     CDS             complement(9588..10331)
                     /locus_tag="VFF22_17220"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZX18595.1"
                     /translation="MPLNIASINIERSKHLPRVEAFVKRERPDLLCLQELCERDIGFF
                     ETLMGGRLHFAPMMLHPEEGPADVVGVGMVARADALTDVAADYYSGSAQGIREITYFS
                     TQGQRTADPLSIAEVMLSATIQGLRIAVTHLNVTPLGTSTAYQRESAGKLIRLAQAQA
                     QQAGDLLLVGDFNAPRGRATFDLIAAHFIDGIPAHYTSSIDGSLHRAGDIPFMVDGLF
                     HTPGYRLENVHLSTGVSDHCALSCRLNKA"
     gene            complement(10632..12758)
                     /locus_tag="VFF22_17225"
     CDS             complement(10632..12758)
                     /locus_tag="VFF22_17225"
                     /inference="COORDINATES: protein motif:HMM:NF024642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZX18596.1"
                     /translation="MTPNERAELEIQKSIESYIDDFKSFRFNAGAGAGKTYALIETLK
                     YVTINKIAATKSSQKVACITYTNVAVNEINTRLGNSETAHVSTIHERLWEIIKIAQPQ
                     LLICHKEKIVAVIKQINQDLSLSEKAKFFTGLDKVQQQEFIEFAKQTKELFYHSKKLN
                     SEFFRAAYKNTGINQPAFLDTCLKNLEKFKFLVSLLYKKQRLEECLERIDAGEENRVS
                     YDSKVNLDRLHYMKFSHDTLLEYGLKLVENYPTLCRIIIDSYPYFFIDEYQDTHCNVV
                     KFLNILHDYAIKNNRNWMVGYFGDTAQSIYDDGVGRNIEVLHAGLKNIDKIFNRRSHQ
                     QIINTANNIRADNIVQVPIFKERNTGSVLFSHNNSEDKLATARSFLAEYRNDLIKENG
                     TQEDLADDENKIHCLVLTNKLMASFNGFGDVYDVYQKSSIFHDNLNTQVLSQQLEKLH
                     PTILTIYRLINFYQDIQQGEISYHDVFGSSGKNMAFSKACLVIGELKNEKVLLLKDWT
                     NLIIERLENSHVKKELAKALINRINYEQDKLSSGDVFRETLSDSINTLMNEDSEDEDK
                     ANEKIDSILSLPIASLTNWVNFIKGIEADEISYHTYHGTKGEEYKNVAIILEHSFGSK
                     NRDKFKNYFKVVQQEVKGIELLFANPEQEDKHINTKNLLYVACSRAIKNLRVLYLDDI
                     SEIKYGIEAIFGKCKPWPEDRTHSPT"
     gene            complement(12814..15072)
                     /locus_tag="VFF22_17230"
     CDS             complement(12814..15072)
                     /locus_tag="VFF22_17230"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZX18597.1"
                     /translation="MYLESLTIKNYRKFRSTDNTVCFVKPKIINMSTEGNQPESVIAP
                     STTLIIGKNNSGKTTVVNALKMLCQNEQPSASDFNLSYLNELFNQYKQDFEENQKTKF
                     ENLETPNLEFTLKIKVDFNDEDLITHLAPFMSISEKSNGEPVSVCIKVKVVLTEESAF
                     KAKIKEILETIDSENNKLDGTQQFSKFYKLLSKENNPYQTAGNIYQFKFIDLIGDATE
                     KFSLKELIHIKEIKANRHLKDGVLSNVFNKIVYFQFTNDVKSRSKLEASIESINRLIS
                     DDVGSKSGDISKVLGEVESSNKVEMSLSGNVTYDAIMKGLIKYNFMDGNDYIPEDQFG
                     LGYINLLNIIGEIIHFVDSYEDKSHFSRINLLFIEEPEAFMHPQMQEFFIKRIDNAVI
                     KALEIANASSAHAKTLQCQIAITTHSSHIVNSKIHSSNTFNNLNYLTSVNKCTKAINL
                     TDDAVVSGDDIDANKKLNFIKKHIKYKVSELFFSDAIIFVEGITEETLLSYYLERHHT
                     LSKHYISVFNINGAHGKLYLPLAKKLSIPSLIVTDLDIKREKCEKNEKHKDNESCGVC
                     SHKAKTDKTPCVPGNAPEYNQITSLNGRKTTNATLKAFNQQIGNKLESEADVIEDIPF
                     FEDDNLYVVFQKYPIEKQYATSLEEALILTNYQNDIINNVIADCKPEIYDEIINTGGK
                     EARTNLIHNSYKLQKKLSDSKSDFSNKLLFSFLSSVEGEDLPKLPNYLESGLDWLVTK
                     LQTNDALSSIKT"
     gene            complement(15281..16387)
                     /locus_tag="VFF22_17235"
     CDS             complement(15281..16387)
                     /locus_tag="VFF22_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18598.1"
                     /translation="MQYKYHNSTILKRKNISDEIIERIINNSLSIDIAMAINFTDFHP
                     GEEFCDDCDACFDALHNTQLIVNFITGKIDRQMVAIQKQQWNYSFLTDKAVHGLGDMS
                     QLSINREAIVLTGTTCYIDQNILTQAVNKLEFFELLTKARIKHDLIIIGSVSHFEEIF
                     KITNVERRDKFVEVLMSLSDGVNLQPCNIDDRIKPFFESAPLILSRIEMTAASSEAVE
                     MLKNLKDEDRRLYFEKYNDLEYRKRIGSSADIFNELTESEFSELVCMSSPPGHLKSDF
                     KNLVHHEEIRDAIYSLHNTMDLMSYKQDKSSRTQRSSAHDIEHLIYGSQCDYFVTNDS
                     NLRQRATEIYRFLEFPVRVLSLSEISSLLDSEWA"
     gene            complement(16641..16940)
                     /locus_tag="VFF22_17240"
     CDS             complement(16641..16940)
                     /locus_tag="VFF22_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18599.1"
                     /translation="MTLENKKYTSAISIVTAIGAFSVAGISIYSGYDLFRLGVETKVA
                     ISASSTSESIELYAITPGIAFLFFGAYISRKALNTLIGSKGVINHFFEWLNKSKA"
     gene            complement(17184..17522)
                     /locus_tag="VFF22_17245"
     CDS             complement(17184..17522)
                     /locus_tag="VFF22_17245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18600.1"
                     /translation="MPWTIENALNDDAIERTEETPTGYSFWLKGIPTEIRVVLAVNPT
                     NCGFNFYLSHYIHTPTQAGKYCPSRPWGDYEAYALHLAVTAITQHYKEAITAGHKPNE
                     NWLIANQNSI"
     gene            complement(17945..18864)
                     /locus_tag="VFF22_17250"
                     /pseudo
     CDS             complement(17945..18864)
                     /locus_tag="VFF22_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485565.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            19036..19758
                     /locus_tag="VFF22_17255"
     CDS             19036..19758
                     /locus_tag="VFF22_17255"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZX18601.1"
                     /translation="MRLALWQTQGFPADVDANLKALEPQMQAAAAAGAQVLLCPELWL
                     GGYQVPEQMAALAEAADGPAAQRLADLCREHGLALCYGYAERHPAGGKPYNSAQLIDA
                     QGQRLANYRKTHLFGAMERELFTPGDGFEVPVLLNGWRVALLICFDVEFPEAVRSHAL
                     VGAELVLIPTALTPEYRVVPELIVPIRAVEHQLFVAYCNHSGVENGLAFLGGSCVAAP
                     DGERFGALAGGEGVLVVDLDRS"
     gene            complement(19890..20657)
                     /locus_tag="VFF22_17260"
     CDS             complement(19890..20657)
                     /locus_tag="VFF22_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003174732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18602.1"
                     /translation="MPRTYLPSRLLIGLSLSGAAVTAAHAESITDPISTLGVIGSHKQ
                     YKLTVDDDSDKERLNLGGLFYNFGNKMTGQEGPIYQIGIEGQYREKDDTEYKTARADL
                     DLGLRAALSTNNYIDVLVGGGYDWGRTEQDDVQVGLFEEDVKLTTRSPFAKAGLGYNY
                     LTPDYTVRLELGARYSINAESKLKIGDESDTVDLKDKVNPYGELSVLWNKGINNLPVS
                     TSLYYSQTRYELDSSSAVAERTKLKQEEVGLKIGLAF"
     gene            complement(20840..22180)
                     /locus_tag="VFF22_17265"
     CDS             complement(20840..22180)
                     /locus_tag="VFF22_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZX18603.1"
                     /translation="MDPSTSYVASNYFADFGLVLFALFLVLLNGFFVAAEFAIVKLRA
                     TKVDALAAKNGWRGHILRTVHNQLDAYLSACQLGITLASLGLGWVGEPAFAHLLEPLL
                     AGIGIESPKLIHGIAFFTAFFIISYLHIVVGELAPKSWAIRKPELLSLWTAAPLYLFY
                     WAMYPAIFLLNASANAILRVAGQGEPGPHHEHHYSRDELKLILHSSRASDPSDQDMRV
                     LASAVEMGELEVVDWANSREDLVSLPLTAALDEVFSVFRRHKYSRYPIYDDTSGEYVG
                     VLHIKDLLLHLSLLEMLPSALKLAELMHPVERVGRHMPLSTLLEQFRTGGSHFALVEE
                     ADGKVIGYLTMEDVLEALVGDIQDEHRKAERGILAYQPGKLLVRGDTPLAKVERLLSV
                     DLDHVEAETLAGLIYDTLKRMPEEEEVLEVDGLRIIIKKMKGPKIVLAKVLKLD"
     gene            complement(22250..23557)
                     /gene="phoR"
                     /locus_tag="VFF22_17270"
     CDS             complement(22250..23557)
                     /gene="phoR"
                     /locus_tag="VFF22_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HZX18604.1"
                     /translation="MNQDWRGPLIRRLLLLVGVCLLLGLITGEYAWTLLLGLAGYLGW
                     HLQQLLRLHKWLRTRQGDEAPPDGYGLWGEVFDSIYHLQRRDQKVRGRLQAVIDRVQE
                     STAALKDAVIMLDRDGNLEWWNIAAEKLLGLKTPQDSGQQITNLVRDPRFIEYFENHN
                     YNEPLELPSPVSDRLRLQFHITQYGNREHLMLVRDITRLYQLEQMRKDFVANVSHELR
                     TPLTVIAGYLETLLDNVENVNPRWLRALQQMQQQGGRMQNLLNDLLLLAKLEATDYPS
                     DNQPVAVDLLLLSIKNDAQALSSERHHRISLEADPHLKLKGSETELRSAFSNLVFNAV
                     KYTPDEGEIRIRWWSDEQGAHLSVQDSGLGIEAKHLPRLTERFYRVDSSRASNTGGTG
                     LGLAIVKHVLLRHRARLDIVSKLGKGSTFTCHFPTLQVVRRGQ"
     gene            complement(23626..24315)
                     /gene="phoB"
                     /locus_tag="VFF22_17275"
     CDS             complement(23626..24315)
                     /gene="phoB"
                     /locus_tag="VFF22_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HZX18605.1"
                     /translation="MVGKNILIVDDEAPIREMIAVALEMAGYECLEAENTQQAHAIIV
                     DRKPDLILLDWMLPGTSGIELARRLKRDELTGDIPIIMLTAKGEEDNKIQGLEVGADD
                     YITKPFSPRELVARLKAVLRRAGPTDSEGPIEVGGLLLDPVSHRVTIDGKPAEMGPTE
                     YRLLQFFMTHQERAYTRGQLLDQVWGGNVYVEERTVDVHIRRLRKALGEVYENLVQTV
                     RGTGYRFSTKS"
     gene            complement(24407..25300)
                     /gene="ubiA"
                     /locus_tag="VFF22_17280"
     CDS             complement(24407..25300)
                     /gene="ubiA"
                     /locus_tag="VFF22_17280"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003367996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HZX18606.1"
                     /translation="MYTRLLHSLNRLHPRTWDFIQLMRLDKPIGIYLLLWPTLWALWV
                     AAEGVPSAKNLFIFIVGVFLMRAAGCVINDYADRNFDGHVSRTKARPLASGKIQPREA
                     LILFTVLVAASFVLVLFTNATTIWLSFGGLALAACYPFMKRYTFYPQVVLGAAFSWGM
                     PMAFTAETGTLPPEAWLLYIANLLWTVAYDTYYAMADREDDLKIGVRSTAILFGDADR
                     LIIVILQGLALLCLLLAAARFELGLNFHLGLLAAAGCFAWEFHKTRNRKPMACFNAFL
                     HNHWAGLAIFIGLVLDYALRG"
     gene            complement(25406..25957)
                     /locus_tag="VFF22_17285"
     CDS             complement(25406..25957)
                     /locus_tag="VFF22_17285"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012020163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HZX18607.1"
                     /translation="MPHPTLAYPPRWLTASQLHPAPTAAVRDWLFNEDSLTRRLTALS
                     ADGFSVTPLQEGWQRLRNDECSALGVVNGSQGWVREVYLRGHGRPWVFARSVAARSVL
                     EGSSLTLDQLGSRSLGELLFSDRAFDRGELQVCRYPSQWLPAEACSERLWARRSCFSR
                     GALGVLVAEVFLPEFWATAAIEP"
     gene            26173..26340
                     /locus_tag="VFF22_17290"
     CDS             26173..26340
                     /locus_tag="VFF22_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HZX18608.1"
                     /translation="MKKWQCVVCGLIYDESQGWPDEGIAAGTLWQDVPEDWLCPDCGV
                     GKLDFEMIEIG"
     gene            26440..26607
                     /locus_tag="VFF22_17295"
     CDS             26440..26607
                     /locus_tag="VFF22_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HZX18609.1"
                     /translation="MRKWQCVVCGFIYDEALGLPDEGIAPGTAWEDIPADWLCPDCGV
                     GKLDFEMIEIA"
     gene            26664..27818
                     /locus_tag="VFF22_17300"
     CDS             26664..27818
                     /locus_tag="VFF22_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZX18610.1"
                     /translation="MSMSAPVVIVGTGLAGYNLAREFRKLDSETPLLLITADDGRSYS
                     KPMLSTGFAKNKDADGLSMAEPGAMADQLKAEVRTHTRISGIDPGHKQLWIGEEAVPY
                     RDLVLAWGAEVIRVPVQGDADDAIFSINDLEDYARFRTAAAGKRRVLLLGAGLIGCEF
                     ANDLSLGGYQVELVAPCEQVMPGLLHPAAAAAVQAGLEGLGARFHLGPVLASLNHQGD
                     ALQAHLSDGTLIECDLVLSAVGLRPRIDLAAAAGLAVNRGVVVDRQLRTSHANIYALG
                     DCAEVDGINLLYVMPLMTSARALAQTLAGIPTAVSYGPMPVTVKTPVCPLVVSPPPRG
                     LQGEWSVEGSGADIKALCHDSSGALLGYALTGTAVQEKLALNKLLPALLA"
     gene            28005..28277
                     /locus_tag="VFF22_17305"
     CDS             28005..28277
                     /locus_tag="VFF22_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZX18611.1"
                     /translation="MRKPELAAAIADKADLTKDQANRVLNAVLEEITNALNRKDSVTL
                     VGFGTFVQRHRGARTGKNPQTGEPVKIKASNTVAFKPGKSLKDAVN"
     gene            28418..28810
                     /locus_tag="VFF22_17310"
     CDS             28418..28810
                     /locus_tag="VFF22_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HZX18612.1"
                     /translation="MKFRFLLWMLGRLMAKASRENPAFQQQLLDRDMVFQLHTLDGKV
                     ARHFIVKDQRITSKRGPATEPAFALGFKDAAYGFATMSAKNKQLAFMQGIQNKDIQIQ
                     GNPALVIWFQGLTKYLMPKKKDAAKKAA"
     gene            28890..29627
                     /locus_tag="VFF22_17315"
     CDS             28890..29627
                     /locus_tag="VFF22_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18613.1"
                     /translation="MRLLLSALLFSTSLAHAAPPKAEAFAPLFELAGIRLLCEQSAPL
                     VQRGLTDKQQAQLAKVFAADGLCLDLAKRLTSKLDQAQLQHAQSLLESPLAQGFTAAE
                     RSVGEGGAEGLAAYRKQLEERPPREERLALVRRLDAAAHTTTLATLLRYEVGKTQALL
                     ALQSRGESIDEKAMSSQTKAQEDALRASSSEAVESFMLYAYRQIPSEKLAEYAALYEQ
                     PAVAQLLGSSVQVLPELFAARRAQLKK"
     gene            complement(29683..31089)
                     /locus_tag="VFF22_17320"
     CDS             complement(29683..31089)
                     /locus_tag="VFF22_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HZX18614.1"
                     /translation="MTELALATDTAPRPPAVILQLLEKLGLPYQICPDQPGLPAAQRL
                     QAVLLDDAVGALLVLYPQSQLLDLNRLTELTGRQLTAVKPERLERMLGKHSLKVLPGL
                     PPLTSSPCLYDERLLQVPSLYIDSGQRGVLLELSTEAFKTLLSKASAARFGEPLSNIR
                     LNLDRPDDDRAEITLAVQAFTARRIQQRLEETIEIPPLPETAQKIIKLRVDPDATVDD
                     ITGVVETDPALAAQVVSWAASPFYAAPGKIRSVEDAIVRVLGFDLVINLALGLALGKT
                     LSLPKDHPQQATPYWQQAIYSAAVIEGLTRAIPRAQRPEAGLTYLAGLLHNFGYLVLA
                     HVFPPHFSLICRHLEVNPHLSHSYIEQHLLGISREQIGAWLMRYWDMPEELASALRFQ
                     HDPEYTGPHATYPNLVCLSVSLLRNRGIGAGQQSEIPQSLFDNLGISREKAEEAVSKV
                     LAAEVALRELAAQFHPAH"
     gene            complement(31180..33255)
                     /gene="recG"
                     /locus_tag="VFF22_17325"
     CDS             complement(31180..33255)
                     /gene="recG"
                     /locus_tag="VFF22_17325"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HZX18615.1"
                     /translation="MTELAAVSVTALKGVGAALAEKLAKVGLETLQDVLFHLPLRYQD
                     RTRIVPIGALRPGQDAVVEGIVAGADVVMGRRRSLLVRLQDGSGTLSLRFYHFSQAQK
                     EGLKRGTLVRCYGEVRPGSSGLEIYHPEYRALSGSEPIAVEQTLTPIYPTTEGLTQQR
                     LRQLSEQALARLGPHSLPDWLPRELADDYQLSPLDQAIRYLHRPPPDADLEELAEGRH
                     WAQHRLAFEELLTHQLSLQRLRESVRAQLAPALPLAKSLPQQYLANLGFTPTGAQQRV
                     GAEIAYDLSQSEPMLRLVQGDVGAGKTVVAAFAALQALEAGYQVALMAPTEILAEQHF
                     INFSKWLAPLGLEVAWLAGKLKGKARASALEQIAAGAPMVVGTHALFQDEVQFQKLAL
                     VIIDEQHRFGVQQRLALRQKGIGGRMCPHQLIMTATPIPRTLAMSAYADLDTSILDEL
                     PPGRTPVNTLVIADSRRLEVVERVRLACNEGRQAYWVCTLIEESEELTCQAAETTFED
                     LSAALGGLNVGLIHGRMKPAEKAAVMDQFKQGQLQLLVATTVIEVGVDVPNASLMIIE
                     NPERLGLAQLHQLRGRVGRGSAASHCLLLYHPPLSQLGRERLAIMRETCDGFVIAEKD
                     LELRGPGEMLGTRQTGLLQFKVADLMRDADLLPAVRDAAQALLEHWPQHVSPLLERWL
                     RHGQQYGQV"
     gene            complement(33264..34190)
                     /locus_tag="VFF22_17330"
     CDS             complement(33264..34190)
                     /locus_tag="VFF22_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HZX18616.1"
                     /translation="MTLTELRYIVTLAQEQHFGRAAERCHVSQPTLSVGVKKLEDELG
                     VLIFERSKSAVRLTPVGEGIVTQAQKVLEQAQGIRELAQAGKNQMAAPLKIGAIYTVG
                     PYLFPHLIPQLHRVAPQMPLYIEENFTHVLRDKLRNGELDAIIIALPFHEADVLTKPL
                     YDEPFYALLPAGHPWAAMKTIDSKLLNDKSLLLLGEGHCFRDQVLEACPTLRKGGEDS
                     AKHTTVESSSLETIRHMVASGLGVSILPFSAVDSHHYAPGVIEVRPLSAPAPFRTVAI
                     AWRASFPRPNAIEVLTDSIRLCSVAKPLPKSS"
     gene            34451..34864
                     /locus_tag="VFF22_17335"
     CDS             34451..34864
                     /locus_tag="VFF22_17335"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZX18617.1"
                     /translation="MPDAVTTISHNWAFAVFLLGVCGLIAFMLGLSSLLGSKAWGRSK
                     NEPFESGMLPVGSARLRLSAKFYLVAMLFVIFDVEALFLFAWAVSVRESGWAGLIEAT
                     VFIAILLAGLVYLWRIGALDWAPQGRRERQAKLKQ"
     gene            34875..35552
                     /locus_tag="VFF22_17340"
     CDS             34875..35552
                     /locus_tag="VFF22_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HZX18618.1"
                     /translation="MHYKLTRIDPDAPNERYPVGERETVSDPLLDDQVHKNIYMGKLA
                     DVLNGAVNWGRKNSLWPYNFGLSCCYVEMTTAFTAPHDVARFGAEVIRASPRQADFMV
                     IAGTCFIKMAPVIQRLYEQMLEPKWVISMGSCANSGGMYDIYSVVQGVDKFLPVDVYI
                     PGCPPRPEAFLQGLMLLQESIGQERRPLSWVVGDQGVYRADMPSQKEQRREQRIAVTN
                     LRSPDEV"
     gene            35609..37390
                     /gene="nuoC"
                     /locus_tag="VFF22_17345"
     CDS             35609..37390
                     /gene="nuoC"
                     /locus_tag="VFF22_17345"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HZX18619.1"
                     /translation="MTADSILSIPPYKADDQDVVVELNSRFGADAFTAQSTRTGMPVL
                     WVARERLVEILTFLRNLPRPYVMLYDLHGVDERLRTQRRGLPGADFTVFYHLMSLERN
                     SDIMLKVALSERDLNVPSVTSIWPNANWYEREVWDLYGIHFSGHPHLTRIMMPPTWEG
                     HPLRKDYPARATEFDPFSLTLAKQQLEEEAARFKPEDWGMKRGSEHEDYMFLNLGPNH
                     PSAHGAFRIILQLDGEEIVDCVPEVGYHHRGAEKMAERQSWHSFIPYTDRIDYLGGVM
                     NNLPYVLAVEKLAGITVPQRVDVIRIMMAEFFRITSHLLFLGTYIQDVGAMTPVFFTF
                     TDRQRAYKVIEAITGFRLHTAWYRIGGVAHDLPRGWDRLVKEFVDWLPKRLDEYEKAA
                     LQNSILKARTIGVAQYNTKEALEWGTTGAGLRATGCDFDLRKARPYSGYENFEFEVPL
                     AANGDAYDRCMVRVEEMRQSIKIIDQCLRNMPEGPYKADHPLTTPPPKERTLQHIETL
                     ITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSFPHLQQIP
                     SVIRGSMVADLIAYLGSIDFVMADVDR"
     gene            37387..37887
                     /gene="nuoE"
                     /locus_tag="VFF22_17350"
     CDS             37387..37887
                     /gene="nuoE"
                     /locus_tag="VFF22_17350"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HZX18620.1"
                     /translation="MIMSTLIQTDRFILSEAEHAAIEHEMNHYEDPRAASIEALKIVQ
                     KARGWVPDGAADAIGEILGIPASDVEGVATFYSQIFRQPVGRHIIRVCDSMTCYVGGH
                     ESVVAEMQKQLGIGLGETTNDSRFTLLPVCCLGNCDKAPALMIDDDTFGDVQPDGVAK
                     LLEAYV"
     gene            37884..39239
                     /gene="nuoF"
                     /locus_tag="VFF22_17355"
     CDS             37884..39239
                     /gene="nuoF"
                     /locus_tag="VFF22_17355"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HZX18621.1"
                     /translation="MSIKTLTSIGPANKIARSEESHPLTWRLRDDAQPVWLEEYQQKN
                     GYAAARKALSEMAQADIVQTVKDSGLKGRGGAGFPTGVKWGLMPADESMNIRYLLCNA
                     DEMEPNTWKDRMLMEQLPHLLVEGMLISARALKSYRGYIFLRGEYVDAAANLNRAIEE
                     AKAAGLLGKNILGSGFDFELFVHTGAGRYICGEETALINSLEGRRANPRAKPPFPAAV
                     GVWGKPTCVNNVETLCNVPAIVGNGVDWYKSLARPESEDHGTKLMGFSGKVKNPGLWE
                     LPFGVSARELFEDYAGGMRDGYTLKCWQPGGAGTGFLLPEHLDALMYAGGIGKVGTRM
                     GTGLALAVDDSISMVSLLRNMEEFFARESCGWCTPCRDGLPWSVKILRALERKQGTQQ
                     DIDTLLGLVNFLGPGKTFCAHAPGAVEPLGSAIKYFRSEFEAGVASSAAPATTGQFVP
                     A"
     gene            39335..42055
                     /gene="nuoG"
                     /locus_tag="VFF22_17360"
     CDS             39335..42055
                     /gene="nuoG"
                     /locus_tag="VFF22_17360"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HZX18622.1"
                     /translation="MATIHVDGKDFEVDGADNLLQACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYTDENDTRGRIVMSCMTPATDNTWISIDDEESKAFRASVVEWLMTNHPHD
                     CPVCEEGGHCHLQDMTVMTGHNQRRYRFTKRTHQNQQLGPFISHEMNRCIACYRCVRY
                     YKDYAGGTDLGVYGAHDNVYFGRVEDGVLESEFSGNLVEVCPTGVFTDKTHSERYNRK
                     WDMQFAPSICHGCASGCNTSPGERYGELRRIENRFNGDVNQYFLCDRGRFGYGYVNRS
                     DRPRQPLLVNKDGGAKLGIDAALDKAAELLKGKRIIGIGSPRASLESNFALRELVGEA
                     NYYPGIAAGELDNIRLIRDILQNGPLPVPTLREVELHDAVFILGEDLTQTAARLALAL
                     RQTVKGKATEIATSMKIQDWHMAAVQNVAQDALHPLFIASVTATRLDDIAEACVHAAP
                     ADLARIGFAVAHAIDPSAPAVNGLDADVGELVELIAEALLEAKRPLIISGASLGEKSL
                     IEAAANIASALKNREKNASISLVVPEANSMGLALLGGESVDAALQALTSGQADAVIVL
                     ENDLYRRADAAQVDAALAAAKLVIVADHQHTATSEKAHLLLPVASFAEGDGTLVSFEG
                     RAQRFFQVFEPSYYDANILIREGWRWLHALHSTLQGKPLDWTQLDAVTAAVAASNSLL
                     ARIGEAAPNATFRIKGLKLAREPHRYSGRTAMRANISVHEPRQPQDQDSAFSFSMEGY
                     SGSKEDRQQIPFAWSPGWNSPQAWNKFQDEVGGHLRAGDPGVRLLEAKGVVLPWFSVP
                     AAFNPAPGTLQAVALHHLFGSEETSAGAAPLQERIPAPYALLAKAEADRLGVNDGALL
                     SLTVAGQTLRLPLQITEELAIGVVGLPVGLVGMPALIAGSVVTRIEEVAQ"
     gene            42052..>43020
                     /gene="nuoH"
                     /locus_tag="VFF22_17365"
     CDS             42052..>43020
                     /gene="nuoH"
                     /locus_tag="VFF22_17365"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HZX18623.1"
                     /translation="MSWLTPEVLDVLIAVLKAIVILLVVVICGALLSFVERRLLGWWQ
                     DRYGPNRVGPFGMFQIAADMIKMFFKEDWTPPFADKFIFILAPMIAFAAMLMAFAIIP
                     ITPNWGVADLNIGILFFFAMAGLSVYAVLFAGWASNNKFALLGALRASAQTVSYEVFL
                     ALALMGIVAQVGSFNMRDIVDYQAENLWFIIPQFFGFCTFFIAGVAVTHRHPFDQPEA
                     EQELADGYHIEYAGMKWGMFFVGEYIGIITISALLVTLFFGGWHGPFGILPQIPFFWF
                     ALKTCFFIMIFILLRASIPRPRYDQVMAFSWKFCLPLTLINLLVTGA"
BASE COUNT         8696 a        12148 c        12749 g         9427 t
ORIGIN      
        1 tcatgcggcc tacagatact tggccccgtt caatccaaca ctagtctgct aggagtaagg
       61 catgaaactt aagcgtttga tggcggccat gacttttgtc gccgctggcg tggctaccgc
      121 cactgcggtt gctgctgtcg acccggctct gccgagctac accaagacct ccggcgtatc
      181 gggcaacctg tccagcgttg gttccgactc cctggccaac ctgatgactc tgtgggctga
      241 agagttcaaa aaggaatacc cgaacgtcaa catccagatc caggccgccg gctcgagcac
      301 cgcgccgccc gctctgactg aaggcaccgc cagcatgggc ccgatgagcc gcccaatgaa
      361 ggatgccgag atccaagcgt tcgaagagaa atacggctac aagccgactg ccgtaccggt
      421 ggccatcgac gccctggcgg tattcgtgca caaagacaac ccgatcaaga gcctggacat
      481 ggctcaggtt gacgcaatct tctccagcac ccgcctgtgc ggtggcgcta ccgatatcaa
      541 aacctggggc gacctgggca tgactggcga gtgggcggcc aagccgatcc agctgttcgg
      601 tcgtaactcg gtatccggca cttatggcta cttcaaggaa gaagccctgt gcaaaggcga
      661 ctacaaggct aacgtcaacg agcagccagg ttcggcttcc gtggtgcagt cgatctccag
      721 caccatcaac gccatcggtt actcgggtat cggctacaag acttccagcg tgaagactgt
      781 gccgctgtcg aagaagggcg gcgaagcctt tgacgccaac gaagaaaacg ctctggctgg
      841 caagttcccg ctggctcgct tcttctacgt ttacgtcaac aaggcgccga acaagccgct
      901 gagcccgctg gacgccgagt tcgtcaagct ggttctgtcc aagcaaggcc aggaagttgt
      961 cgtgaaagac ggctacatcc cgctgccgag caaagtggct gcgaaaaccc tgaaggaatt
     1021 gggcctggag taatcccggt acaggcaagg acgcctaagt aagtcgcacg cccacctccg
     1081 tttacgacag gtgggcgttg ctttgtcaaa gcgctgtaac ttttctgtca catagggcgg
     1141 ctagagttag cgcctcgtat agcgacagaa atcactatgg cgcgctcatg gctgcgcaga
     1201 gacgctctta cctatgaatg acttggctag tgaaaccatg accgccacat ccaaatctct
     1261 cgggctggac ttcaataccc cagccctgct gcgcaaacgg cgtatccgtg cgctgaaaga
     1321 ccgcttcgcc cgctgggcgg tgtcaatcgg tggtatggcc gtgctcggcg ccatcaccct
     1381 gatcttcttc tacctggcct atgtggtgct gccgatgttc cagggcgcga gcatggacag
     1441 ccgcaagcca gtgcaggttg cctggctgaa tgaagccgcc gcgccgctac tgttgacctt
     1501 ggaagaacag aaccagatgg gcatgcgcct cgaccagaac ggtcaggtgc agttcttcga
     1561 tgcgaagaag atgcagccgc taagcagcgt gcagctgccc atcccggctg atgtgcggat
     1621 tgcctcggtc ggccaggatc agccgggcag caaccgcgtg gccctgggcc tatccaacgg
     1681 tcaggtgctg gtgttcgagc atgtctacaa gctcagctac ccgaatgatg tgcaaaccat
     1741 cactccaagc atcgagtacc cgttcggcga agcgccgatc gagctcgacc ctcagggccg
     1801 cgagctggat cacatgggcg tcagcctgaa cagcggcacg ctgatgctgg cgggctccac
     1861 cggcaccgag ctgcacgtgc tgagcctggg ccgtgaagaa aacatgatga ccggcgaggt
     1921 aaccctcagc gaagagcgtg ttgctctgcc gcagatcgcc gagccgatca aggcattgat
     1981 catcgaccca cgccacctgt ggctgtatgt ggtcaacggc cgcgccactg ccgatgtctt
     2041 cgacatgcgc agcaacagcc tgaatggtcg ctacaagctt ctggaagacg gcaagctgga
     2101 agtcagtaac gccacctcgc tgctcggcgg catctcgctg atgatcggcg ataccagcgg
     2161 cggcatcgcc cagtggttta tggtgcgcga agcagacggc aaggccgtgc tgaaatcgat
     2221 ccgcagcttc cagctggccg atagtccgat catccagatt ctcccggaag agcgccgcaa
     2281 gggcttcttc gccctggacg ccaagggcaa cctgggtatc ttccacagca ccgcgcaccg
     2341 cacgttgctg gtcgagcctg ttgctgacgg ccaggccttg gccaccctgt cgccgcgcgc
     2401 caaccgcgtg ctggtggaga gcgatggcca gttgcagcga ttgatcatcg acaacccaca
     2461 cccggaaatt tcctggagct cgttgtgggg caaggtttgg tacgagaact atgacgcgcc
     2521 gggctacgtt tggcagtcga cgtccgccag caccgacaac gaacccaagc taagcctcgc
     2581 gccgctggcc ttcggtacgc tgaaagccgc gttctacgcg atgctcctgg ctgctccgct
     2641 ggccattgcc gccgcactgt acaccgccta cttcatggct ccggcgctgc gtcgcaagat
     2701 caagccggtg atcgagctga tggaagcgct gccgacggtg atcctcggtt tcttcgccgg
     2761 cctgttcctc gcgccttatg tggaaggcca tctgccgggg attttcagtc tgctgatctt
     2821 taccccgata ggcattctgc tggccggtta cctgtggagc cgtctgccgg agtcgatccg
     2881 cctgagcgtg ccggatggct gggagtcggc gatgctgatc ccggtgatcc tgctggtcgg
     2941 ctatgtctcg ctggccatga gcggccatgt ggagaactgg atgttcgacg gcaatatgcg
     3001 catgtggctg agcaatgatt tgggcattcc gttcgaccag cgcaacgctc tagtagttgg
     3061 tctggccatg ggctttgcgg tgatcccgac catctactcg attgccgaag acgccgtatt
     3121 cagcgtgccg aagagcctga cattcggctc tctggccctc ggtgctacac cgtggcagac
     3181 cctgacccgc gtggtgattc tcaccgccag cccaggcatc ttctcggcgg tgatgatcgg
     3241 catggggcgc gccgttggcg aaaccatgat cgtgctgatg gccaccggca acaccgcgat
     3301 catggacatg aacatctttg aaggtctgcg caccctggct gccaacgtgg cggtggaaat
     3361 gcccgaatcg gaagtcggtg gtactcacta ccgcgtgctg ttcctctcgg cgctggtgct
     3421 gctcggcttc acctttgtca tgaacaccct ggcagagctg gtgcgccagc gtctgcgcgt
     3481 caagtacgcc tcgctctaag gcttgggaag gtatatgtcc gtgaaacaga acaacctgaa
     3541 aacctggttc aagagcggta cgccgtggat ctggatgaat gccggcgcgg tatcgatcgc
     3601 cgtgatcatg accctgggtc tgatgatggt gatcgccgtg cgcggcctcg gccacttctg
     3661 gccggcggat atcgttgagg ccgactacca gatccctggt cagcaagcca aggtgatggc
     3721 cggcgaggtc acccagatcg aagaagtgcc gcgtgcccgt ctggcagctt ccggcttgcc
     3781 ggtggaggct gaaggcggcg agtttatgac ccgcgagcta ttcaaagtgg gtaaccgcga
     3841 cgtgtatggc gcggacttca cctgggtggt cggcgaatgg ctgagcaacc tgcgtactcc
     3901 ggaaaacctc acggcgctgg agcgtcgcga gtggggcaac ttctacggct acctggtcaa
     3961 tgttaaggaa aacggcgaac tggttgctga aggcgacgcc agctggcctg cactgcaaga
     4021 gcgcctcgag cgcgtcgagc agctgcacaa gaacctggtg cgcctggaga agaaagacat
     4081 cgggcggatc aacgccggtc tggaacgcat ccgtctgcag actcgcaagc tggagctgca
     4141 gggcaaactg gacgacgtcg cccaggctga gctggacgcc gagcgtgcgc agtgggatgc
     4201 cgagtacaag gtgctggaaa ccgagctgat cgcccttaat caggcgttca accgcgacag
     4261 cgtcacgctg aaaaccgccg atggccgtga agtgaccatc agcctgggca aggtggtacg
     4321 ggccttccag ccgaatgcca tgggcatggg cgacaagctc ggtttctact tcgccaagct
     4381 gtgggaattc gtcagcgacg acccgcgtga agccaacacc gagggcggca tcttcccggc
     4441 gatcttcggc accgtgatga tgaccctgat catggccgtg atcgtcaccc cgtttggcgt
     4501 ggtagcggcc atctacctgc gtgaatacgc caagcagggc ccgctgaccc gggtgatccg
     4561 catcgcggta aacaacctgg ccggcgttcc tgccatcgtt tacggcgtgt tcggcctggg
     4621 tttcttcgtc tatgtactgg gtggctctct ggaccgtatg ttcttcccgg aagccgcacc
     4681 ggcgccgacc ttcggtacgc cgggcctgct gtgggcctcg ttgaccctgg cgatcctcac
     4741 cctgccggtg gtgatcgtgg ccaccgaaga aggtttggcg cggattcctc gcgcagtgcg
     4801 tgaaggctct ctggccctcg gcgcgaccaa ggtggaaacc ctctggcgcg tggtgctgcc
     4861 aatggccagc ccggcgatga tgaccggcct gattctcgcc gtggcccgcg ccgctggcga
     4921 agtggcgccg ctgatgctgg tgggcgtggt caagctggct ccgagcctgc cgctggatgg
     4981 caactacccg tatctgcacc tcgatcagaa gatcatgcac ctgggcttcc acatctacga
     5041 cgtcggcttc cagagcccca acgtcgaggc cgcgcggccg ctggtgtacg ccaccgcgtt
     5101 gctgctggtg ctggtcatcg tggtgctgaa catggcggcg gtctatatcc gcaaccacct
     5161 gcgcgagaag tacaaggcgc tggatcacta atcattgcgc cgcggcctgc tggccgcggc
     5221 ggcttaacgc atttgttagc gtagggagca tcccatgcag cacgaaacac atacacacgg
     5281 tatcgacatg gcggccctcg gccgcgaccg ccagggcctg aacatggcca acgagcgcgt
     5341 ggccatcgac gtacccggtc tgaacctcta ttacggcgag aagcaggcgc tgttcgacgt
     5401 caagatgacc atccccaacc agcgcgtgac ggcctttatc ggcccgtctg gttgcggcaa
     5461 gtcgaccctg ctgcgcacct tcaaccggat gaatgatctg gtcgacggtt gccgcgtgga
     5521 aggcgaaatc aacctcgacg gcaagaacat ctaccgcaag ggcgaagacg ttgccgagct
     5581 gcgtcgtcgc gtcggcatgg tgttccagaa gcccaacccg ttccccaaga gcatctacga
     5641 gaacgtggtc tacggcctgc gcatccaggg catcaaccag aagcgcgtgc tcgatgaagc
     5701 cgtggaatgg gcactgaaaa gcgcggcact gtgggatgag gtcaaggacc gtctgcacga
     5761 gtcggcactc ggcctctccg gtggtcagca acagcgcctg gttatcgccc gtaccgtggc
     5821 ggtgcagccg gaagtactgc tgctcgacga accttgctcg gccctcgacc cgatctccac
     5881 gctgaaagtc gaagagctga tctacgaact gaaatccaag tacaccatcg ttatcgttac
     5941 ccacaacatg cagcaggcgg cgcgggtttc ggattacacg gcgtttatgt atatgggcaa
     6001 actgatcgag ttcggtgata ccgatacgct gttcaccaac ccggcgaaga aacaaaccga
     6061 agattacatc accggtcgtt acggttgatg gcaagcccat cctgagcaga gacgttattc
     6121 ggttaattca agggttagca catgatcaac aaagaaaacc ttacccagca tatctcccag
     6181 cagttcaacg tcgagctgga ggaggtgcgt agccacctgc tggccatggg cggcctggtc
     6241 gagaagcagg tcaacgacgc ggttaccgcg ctgatcgacg ccgactccgg tctggctcag
     6301 caagtgcgcg agatcgatga ccagatcaac cagatggaac gcaatatcga cgaagagtgc
     6361 gtgcgtattc tcgcccgtcg tcagccggca gcgtccgacc tgcgtctgat catcagcatc
     6421 tccaaatcgg tcatcgatct ggaacgcatc ggtgacgaag cgaccaagat cgccaagcgc
     6481 gcgatcctgc tgtgcgaaga aggcgaagcg ccgcgcggtt atgtggaagt gcgacatatc
     6541 ggcgatcagg tgcgcaagat ggtgcaagag gcgctggacg ccttcgcccg tttcgacgcc
     6601 gacctggcgc tgtcggtggc gcagtacgac aagaccgtcg accgtgaata caagacagcg
     6661 ctgcgtgaac tggtgacttt tatgatggaa gacccgcgct caatctcgcg cgtgctcaac
     6721 atcatctggg ccctgcgttc gctggagcgc atcggcgacc acgcgcgcaa tatcgccgag
     6781 ctggtgatct acctggtacg cggcaccgac gtgaaacaca tcggcctgac ccgcatgcag
     6841 gaagaagtgc agggcaccaa aaaaggcaac tgagtcggct gttgtgggcg gcgttagcgc
     6901 gctgcctgta ctgccctcca tggccaagat gcacctgatc gcgacatagg gtgcatttcg
     6961 gttggccttt ggccagtagc cgtgactatg cttaaggcca tcagaacagg agtggccgat
     7021 gagcaaagtc agcgtgctgg tagtggacga cgcaaccttt atccgcgatt tggtgaaaaa
     7081 ggggttgcgc gaccactttc caggcatcca gatcgaggaa gccgtcaacg ggcgcaaagc
     7141 cctgcagatg ctgactcgcc agccggttga cctgattctc tgtgactggg aaatgccgga
     7201 aatgtcgggt ctggaactgc tcacctggtg ccgcgaacaa gaagccctga agatcgtccc
     7261 gtttatcatg gtcaccagcc gcggtgataa agagaacgtg gtgcaggcga ttcaggccgg
     7321 tgtttccgac tttatcggca aacccttttc caacgaacaa ttgatcacca aggtgaaaaa
     7381 ggccctcagc cgcgccggca aattgcaggc gctgatgtcc agcgcgccgc cgaaaatgct
     7441 cagcagcggc tcttttgcca acgactcgct gtctgccctg accggtggca aggccgatgt
     7501 agttaagccg acagctgggc cggcagtgca agccgccgct gcctttgccg gtaaacccgc
     7561 ggcggccaaa gtacccgctg ccagcgctac cagcggtcgg ggtcagggcc agttgcgctt
     7621 gcccggtggc gccacaatgt cctgcgtgat caaggcactg agcctcaagg aagcactgct
     7681 ggtggtcaaa cgcactgagg tgctgccgca ggtgctggaa agcgcggtgc tcgatctgga
     7741 gcagggcgaa gcggcggaaa ccgcacggct caatggctac ctgcatgccg tcgcggcctt
     7801 cgagccaaaa cctgacagcg agtggctgca actgaccttc agattcgtcg accgcgaccc
     7861 gcagaagctc gactacatct cccggctaat cgcccggggt actgcgcaga agcacttcag
     7921 ccccggcgcg taagcaaacc cagtcctacc ggtgcatgcg agatggctca caaccctggc
     7981 gcgccggttc gcttagcgcg ggatcggctg ctaatctcaa cggtccggac acagacaaat
     8041 cataagatcc gagttgttat gctagggcgt ctgatacttt tctgcagttt gttgaccctt
     8101 gccggccagg cagcggcgtt gactatttac aagtacaccg atgctaacgg cgtggtcacc
     8161 tataccgacc aggccgcgcc cggtgcgcag gtgttcgtgt ttcgcgaccg catggttgag
     8221 cgcctggata accaggtaaa gctggaaacc aagaagcacg aagccggtga aaccctgttg
     8281 gtgcgcaacg atctgcacgc gccggtgcaa atcgagctgc gcttggaagc cactgacaac
     8341 gtcacgggcg tgccggacaa gccgatcagc tgggtgctgc cgccgcgcag caagatccgc
     8401 ctggctaccc tggccccgcg tgatgccagc aaacccatgc gctacacccc caagttgcgc
     8461 tatgccatgg gcgatccgcg cctgctgccg acgcagaaga gttacccgct gccatggcgc
     8521 ggtggcccgt tccgcctgac ccagggcgcc aacggcaaat acagccactt cactcccaaa
     8581 ggccgttacg ccatggatat cgccatgccc gaaggcacgc cgattgttgc ggcgcgcggc
     8641 ggcatcgtgg tgaaaaccga gaatcagcag agcggtcgcg gcaacaatcc gtccggcaac
     8701 tatgtgcgca tcctgcatga cgacggcacc atgggcgtct acttgcacct gatgaagggc
     8761 tcggtggcgg tcagcgaagg cgctcgcatc aacgctggca gcatgctcgc gcgctccggc
     8821 aataccggca acagcaccgg cccgcacctg catttcgtgg tgcagcgcaa tgtcggcctg
     8881 gcgctggagt cgatcccctt caacttcgcc cagccggtca acagcctgcc gaacttcgcc
     8941 gtgggcggcg agtaagcctt gatgcacaac ggcgatgtag ggtgcgccgt gcgcaccgat
     9001 gacctggccc gcctcggcct cggcgtgcgc agcttggtcg aacgttaaca tcgtccccgg
     9061 cgaatgccgc ttcgacttcg agatgcgcgc cctgccagcc ggcgacccca ggcaggttgc
     9121 cgctgagctg caggcctacg ccgagcacga actgctgccg cgcatgcgtg ccgtcagtgc
     9181 cgccagcgac attcgtttca gcgagctatc cagctacccc ggcctgctca ccgacccaag
     9241 tagcgacgct gcgcgcgcga agacttcagc accgtggcct tcggcaccga aggcggcctg
     9301 ttcgacgccg ccggcatcgc caccgtcatc tgcggccccg gcagcatgga ccaaggccac
     9361 aaaccagacg aatacgtcag cctcgcccag ctcaatgcct gcgatgcatt gctgggtaag
     9421 ttggtgaagt ggatgcaggc agagggctga agcagacctt gagggcggga atagaattaa
     9481 aggcgactga gtgtgtaatc agggaaaggg ggggcggatt tatttttgcg tcagctgtat
     9541 tagatcaatc aataaatatg ctctctgttt gttcttttgt tgccggttta ggctttgttc
     9601 aagcggcaac tgagcgcgca atggtcggat actcctgtac tcaggtggac gttttccagc
     9661 cggtagcccg gcgtgtggaa aagcccgtcg accataaacg gaatgtctcc ggcgcggtgc
     9721 agcgagccgt caatgctgct ggtgtagtgc gccggtatgc catcgatgaa gtgcgcggcg
     9781 atcaggtcga aggtggcgcg gccgcgcggg gcgttgaagt cgcctaccag cagcaggtcg
     9841 ccagcctgct gggcttgcgc ctgagccagg cgaatcagct tgccggcact ttcccgctgg
     9901 tatgccgtgg acgtgccaag tggggttacg ttgaggtggg tgacggcgat gcgtaagccc
     9961 tggatggtgg cgctcagcat gacttcggcg atgctcaatg gatcggctgt gcgctggccc
    10021 tgcgtgctga aataggtgat ttcgcgaatg ccctgcgcgc tgcctgagta gtagtcggcg
    10081 gccacgtccg tcaatgcgtc tgcgcgggcg accataccga cgccgaccac atccgcaggc
    10141 ccttcctccg gatggagcat cattggtgcg aagtgcaagc gaccgcccat cagcgtttcg
    10201 aagaagccaa tgtcgcgctc gcagagctct tgcaggcaaa gtaagtccgg gcgttcacgc
    10261 ttgacgaagg cctcgacgcg cggcaggtgt ttggagcgtt cgatgttgat ggacgcgata
    10321 ttgagcggca tgagatcagt aggcgattac ggtatggagg gcagcgtgaa gagcgcaggg
    10381 agtgcttcgc gaacgatgaa aaaccgctgc gctgggttgc cgaagtgtac cagacgcccg
    10441 ccatagaaga ccggcaccct cattgttaca gggctacttc agagcatcct taggcctacc
    10501 attttttaaa tggaatggcg gcaatcaatg gggatcagag gaacatggac gggtttattt
    10561 tcaggtcagt cggattaggc caataaataa agctgtccac ttttgcgatc attggtgatt
    10621 aaaaataaaa ctcatgttgg gctatgagtt ctatcttctg gccaaggttt acactttcca
    10681 aaaatagcct ctattccata tttgatttcc gatatgtcat ccagataaag taccctgaga
    10741 ttttttatcg ctcttgagca ggccacatag agtaagtttt tggtattgat atgtttgtct
    10801 tcttgctctg gattagcaaa tagaagctct attcccttca cctcttgttg tacaaccttg
    10861 aagtagtttt taaatttatc tctgttctta ctgccaaagc tatgctcaag gatgatagct
    10921 acgttcttat actcttcacc cttagttcca tgatatgtat ggtaactaat ctcatcagct
    10981 tcgatacctt taataaaatt gacccagttt gttaaggatg cgattggtaa tgatagaatg
    11041 ctgtctatct tttcattggc tttatcttca tcttccgaat cttcattcat tagtgtattg
    11101 atgctgtctg atagtgtttc tcggaataca tctccagaag acaatttgtc ttgctcgtag
    11161 ttgatgcggt taattagcgc tttagctaat tcttttttta catggctatt ttcaagccgc
    11221 tcaatgatta aattcgtcca gtcttttaat aataagactt tttcattctt taattcgccg
    11281 ataaccaagc aggctttgga aaaagccatg tttttacctg atgatccaaa aacatcatgg
    11341 tacgaaattt ccccttgctg tatgtcttga taaaaattaa taaggcggta gatggttaaa
    11401 atagttgggt gtagcttttc taactgttga gatagaacct gggtgtttag gttgtcatga
    11461 aagattgatg acttttgata aacatcataa acatcgccaa acccattaaa acttgccatg
    11521 agtttatttg ttaataccag acagtggatt ttattctcgt catcagccaa gtcttcttgc
    11581 gttccattct ctttgataag atcatttcta tactcggcta gaaaacttcg agctgtagct
    11641 aatttgtctt ctgagttgtt atgtgaaaaa agcactgatc cagtatttcg ttccttaaag
    11701 attggtactt gaactatgtt gtcggcgcga atattgttgg cagtattgat aatttgctga
    11761 tgcgagcggc gattgaatat cttgtcgata ttttttagac ctgcatgaag cacttcgata
    11821 tttcggccaa cgccatcatc ataaatgctc tgtgcagtat caccaaagta accaaccatc
    11881 caatttctat tatttttaat cgcgtagtca tgaagaatat ttaaaaattt caccacgtta
    11941 cagtgtgtgt cctgatattc atcgataaag aagtaggggt agctgtcaat aattatacga
    12001 caaagagtcg gatagttttc gacgagtttc aaaccatact caagcagagt gtcatggcta
    12061 aatttcatgt agtgaagtct gtctagattt accttactgt cataactcac acggttttct
    12121 tctccagcat caattctctc aaggcactct tcaagtcttt gttttttata tagcaagctg
    12181 acaaggaatt taaatttctc taaatttttc aggcaggtat ctagaaatgc gggctgatta
    12241 attcctgtgt ttttataggc ggctctaaaa aattcagagt ttagtttttt agagtgatag
    12301 aaaagctctt ttgtttgctt ggcaaactca ataaattctt gttgctgtac cttgtcaagg
    12361 ccggtaaaga atttagcttt ctcggagagt gataaatctt ggttgatttg ctttataact
    12421 gccactattt tttctttgtg acatattaaa agctgtggct gggctatttt tattatttcc
    12481 catagtcttt catgaatagt tgaaacgtgt gctgtttcag agttgcctag ccgagtattt
    12541 atttcattca cagcaacgtt ggtataggtg atacatgcta ctttttgtga gcttttggtg
    12601 gctgcaattt tattgatggt gacatatttt agagtttcta tcagtgcgta ggtttttccc
    12661 gcgccggccc ctgcattaaa acgaaaactt ttaaaatcat cgatataact ctcaatgctt
    12721 ttctgaatct ccagctccgc tctctcattt ggcgtcatca gatataatcc ccgtgctcta
    12781 agttttctat tcctagtctt ctacaatctc tacctaggtt ttaattgatg agagcgcgtc
    12841 attggtttgc agtttagtaa ctaaccaatc taaaccactt tctaggtagt ttggtaattt
    12901 cggtaagtct tcaccttcga ctgaagaaag aaaactaaaa agcaatttgt tggagaaatc
    12961 acttttactg tctgatagtt ttttctggag tttatacgag ttgtgaatca ggttagttcg
    13021 tgcttctttt ccacctgtat ttataatttc gtcatatatc tcaggcttac agtctgcaat
    13081 tacattattt ataatgtcgt tttggtaatt ggttaagatc aacgcctctt ctaagctagt
    13141 cgcgtattgc ttttcaattg gatatttttg gaatactaca taaaggttgt cgtcttcaaa
    13201 gaacgggatg tcttcaatta catccgcttc actttcgagt ttattaccta tctgctgatt
    13261 aaacgcttta agtgttgcat tggttgtttt gcgtccatta aggctagtga tttgattata
    13321 ttctggagca tttcctggaa cgcaaggtgt cttatccgtt ttagccttat ggctacacac
    13381 accacaagat tcattatctt tgtgcttttc gttcttctcg catttctcac gcttgatatc
    13441 taagtcggtc acaatcaaac ttggaatgct aagttttttc gccagtggaa gatacaattt
    13501 gccgtgcgcg ccattgatat taaatactga aatatagtgt ttgctaagag tatggtgtct
    13561 ttctagataa taacttaaca gtgtttcttc tgtgatgccc tcaacgaaaa taatcgcgtc
    13621 agaaaagaat agctcagaga ctttgtactt tatatgtttc ttgatgaagt taagcttttt
    13681 gttcgcgtca atgtcatcgc cagaaacaac agcatcatcg gtcaagttta tggctttagt
    13741 gcatttattg actgaggtta aatagtttag attgttgaag gtgttgctgc tgtgaatttt
    13801 actgttgacg atgtgagagg aatgagtggt aatcgcaatc tgacactgta gtgtttttgc
    13861 atgtgctgaa gacgcatttg caatttccag agcctttatt actgcattat caatacgctt
    13921 aatgaaaaat tcctgcatct gagggtgcat gaacgcttct ggctcttcaa taaagagcag
    13981 atttatgcga ctaaaatggc ttttgtcttc atagctatca acaaaatgga tgatctcacc
    14041 aataatattt agtagattga tatagcctaa gccaaattga tcctcaggaa tataatcatt
    14101 gccatccata aaattatatt ttatcagtcc tttcattatc gcatcgtaag taacgttgcc
    14161 cgaaagactc atctcaacct tgttgctgct ttccacctct cctaagactt tagaaatatc
    14221 accgctctta gaacctacat cgtcagatat gagtctgttt atcgactcta tgcttgcttc
    14281 cagttttgaa cgagacttaa cgtcattggt gaactgaaag taaactatct tgttaaaaac
    14341 attgctcagc acgccatctt taaggtggcg attggctttg atttctttaa tgtgtattag
    14401 ctctttaaga gaaaactttt ctgttgcatc tcctatgagg tcaataaatt taaattgata
    14461 aatattaccc gctgtttggt aagggttatt ttcttttgaa agcaatttgt aaaatttcga
    14521 aaactgctgt gttccgtcta gtttgttatt ctccgagtca atggtttcaa gaatttcttt
    14581 gatttttgct ttgaaagcag actcctctgt aagtaccact ttaaccttaa tgcatacact
    14641 aactggttcc ccattgctct tttcactaat tgacataaaa ggagcgaggt gagtaattaa
    14701 gtcttcatcg ttaaagtcca ctttaatctt taaagtaaac tctaagttcg gtgtttctaa
    14761 attttcgaat tttgtctttt gattttcttc aaaatcttgc ttgtattgat tgaataactc
    14821 atttaaatag gaaagattga aatctgatgc cgatggctgt tcattttggc atagcatttt
    14881 taatgcattg acgacagtag ttttgccgga gttattcttg ccgatgatta aagttgttga
    14941 aggtgcaatc actgactctg gttgatttcc ttcagtgctc atattaataa ttttaggttt
    15001 aacgaaacat accgtattgt ctgtagatcg gaacttgcgg tagttcttta tggttagact
    15061 ttctaaatac acgggctttc ttcctataga tagatggtca acgcttcctg aaatctgacg
    15121 cttagttatg ctgttggaat aatgataatt aaaggatggt gatagtctag cggcttgatc
    15181 actcctagtt gatcaaaaat aaatcactcc gtactattag ggtgtatcag attttttaaa
    15241 aaacagtgcc tttaattgga tcaacaagcc tcctgcctag ctaagcccac tccgagtcta
    15301 gcagggaact aatttcagat aacgataata ctcgtacggg aaattctaag aaacgatata
    15361 tctcagttgc cctttgccga agattggaat cattggtaac gaagtaatca cactgtgagc
    15421 cataaattaa atgctcaata tcgtgggcgc tgctacgttg tgttctagag gatttatcct
    15481 gtttgtagga catcaaatcc atcgtattat gcaaggaata gatagcatcc cttatttctt
    15541 catgatgaac taaattcttg aagtcagact ttagatggcc tggcggggaa ctcatgcaaa
    15601 ccaattcaga gaactcactt tccgttaatt cattaaatat atcagcagag cttccaattc
    15661 ttttcctata ttctaagtcg ttgtattttt caaaatacaa tcgcctatct tcgtctttaa
    15721 ggtttttaag catttcaact gcttcgctag acgccgcagt catctcaata cgagagagaa
    15781 tcaatggtgc gctttcaaaa aaaggtttta ttctgtcatc aatattgcac ggctggaggt
    15841 tgacgccatc actaagcgac ataagaacct caacaaattt atctctgcgt tcaacgtttg
    15901 taattttaaa aatttcttcg aaatgcgaga cacttccaat gattattagg tcgtgtttta
    15961 ttcttgcttt cgtcaataat tcgaaaaatt ctaatttatt aaccgcctgc gtcaaaatat
    16021 tttgatctat atagcaggtt gtgccagtga gaacgatagc ctctctgttg atactcagct
    16081 gagacatgtc gcctaagcca tgcactgctt tgtctgttaa aaaagaataa ttccattgtt
    16141 gcttttgtat cgcaaccatt tggcgatcta tttttccagt aataaaatta actatcaatt
    16201 gcgtgttatg aagtgcatca aagcaagcgt cacaatcatc gcaaaactcc tctccggggt
    16261 gaaaatctgt aaaattaata gccattgcaa tgtctatgct tagcgagtta ttaattattc
    16321 tctctataat ttcatctgag atattctttc ttttcaatat agtgctgtta tgatatttgt
    16381 attgcatctt gcttcgctcc agtatttgtc ccggtacacg caacagtgtt ctagatttta
    16441 tttagcgctt tcggccataa aggcggatat aactcatatg caatcatgtt ctatgtagta
    16501 aaaatgggat taagtttcca gaaggttgta tttcaataaa gtcaatagct agttttcatt
    16561 ggcttcaaat ttagcttaca gatgcatcgc agatcgtcat tttcccttgt ggctgcatgc
    16621 cttcctaatg acgcggcttg ttatgctttt gacttattca gccattcgaa aaaatgattg
    16681 atcactcctt tgctaccaat taacgtattt agcgcttttc ttgatatgta agcgccgaag
    16741 aataagaagg caatgcctgg tgtaatagca tatagctcaa tactttcaga agttgagctg
    16801 gcagatattg ctactttagt ttcaacacct agtcgaaata aatcataacc gctatatatc
    16861 gagatccccg caactgaaaa agcgcctata gctgtaacaa tgcttattgc tgatgtgtat
    16921 tttttattct ctaatgtcac gcgatatcct ttaagatgta aagaagtgca acggaaaagg
    16981 ggtgggcttt ttaagattat tcggattaat cgagtgagta tatttgccca atgtcccaat
    17041 gagcgaagcg aggggcggag tgaatgttct agcgatatgg ccacttctgg ttgatcaaaa
    17101 ttaaattaaa ccggcctctt tagctaggtc atgaatattt ctctaactac tctacttgtg
    17161 ggggcagccc atggccgcat attttatatg ctattttgat ttgctatcag ccaattttca
    17221 tttggtttat ggcccgctgt aatggcttct ttgtaatgct gagttattgc tgtaactgcc
    17281 aaatgaaggg cataggcttc gtaatcaccc caaggtctgc tcggacagta ttttccagct
    17341 tgagttgggg tgtgaatgta gtggcttaaa tagaaattaa acccacagtt agtcgggttt
    17401 actgcaagta cgacacgaat ttccgtggga atgcctttga gccagaatga gtaacctgta
    17461 ggtgtttctt cggtgcgttc aatcgcatcg tcgttgagtg cattttcaat tgtccacggc
    17521 atgacgatac ctcctaggta atggtatcta atgtgcagtt gcggctacgc tcgaaaacga
    17581 gccgccaagc agtgttgtgc gagtgcagaa aaggggagag gtgttcgaga gggggatgtt
    17641 gcgcattcca aactcccttt ggttgcggcg ggtggtgtgc tggcaattgg cctgcctgtt
    17701 cgctccgaca ttaagcattt gccgtgcaaa cggcaagcag cgcatgcggg cgctgcttta
    17761 gcgcctgata ggtttctggt ggttgggtgg gttgcggtgg tgcgcagcca acgcgttggc
    17821 cggcgtcagt ctaatttgtc agtccatcac ggcccaggcc tgagcaggtt tgcccggggc
    17881 ggggtatcgc tgcgctcggc tatgtggcga aggtcgccac gcgcagcaga tcatgaacac
    17941 gttcctagcc ctcgctcggc gccgccggcg ctaactgctc cttgcagtaa tccacaaaca
    18001 actgcgccgg cttggtcagt tgtgcgcgct tgagccaggc ggcgaccagg ccggagccgg
    18061 tgacgtcttc gctgatattg acgcagacca cgcgtttacc gtcgtaggtg atatcgctgt
    18121 gcgggcgggt gaccaggacg gagaagccga agccttggcc gaccatgccg cggaccattt
    18181 cgatagaggg tgagctgaag acgatatttg gactcaggcc cagctcctcg aagatgctga
    18241 cgaagtaggt gcggctcggc agcacgtcga ggaggatcat cggttccagc gccaggtcgc
    18301 gcagcgagac ctgggtttgt ccggcaaagc gatggccttc aggtagcagg gcgtaggggc
    18361 gctgcggcgg catcagcggt tcggtttcga tggtgctgtc gaggtcgtgt tcgtagaaga
    18421 tcgtcaggtc gaagcgcccg ccggtcaggc cctgtaccag ctcctgttgt tcgccatcgt
    18481 gcaggcggat ttccacgccg aggtagcgtt ggccgatgcc ggcgatcagg cgcgggagga
    18541 tcagcggggc gacggtctcg aagcagccga tatcgatttg cccggccacc acgtcgttgt
    18601 cggccagggc gttctgctca acctcgtggg ccatgcgcag cagcttttgc gccttgctgt
    18661 agaagcgcgc gccgctgggt gtcagcgaaa caccctgggc gtggtgcgaa taaacagttg
    18721 cacaccgaag ctttcttcca ggcctttgat cgccgtggag atggacggct gggcgatata
    18781 cagcttgcgc gacgcttcgg cgacgctgcc gcactcgacg gtggtgacga agtacttgag
    18841 ttgacgcagg gtataggcag ccacacggaa cctcggtaga acgcttattt atgcctcacc
    18901 ttactccgct cggccaggta accgggctgc ggcggggtga gggttttgct gggcagcggc
    18961 gataaatttc ctggccgctg cgggcatggc ttctgcttgc aaggcccata cacgcttatt
    19021 caaggagacc gcagcatgcg cctggctctc tggcaaaccc agggttttcc cgccgatgtc
    19081 gacgccaacc tcaaggccct ggaaccgcag atgcaggccg ctgccgccgc aggtgcgcag
    19141 gtgttgctgt gcccggagtt gtggttgggc ggctaccagg tgcccgagca gatggctgcg
    19201 ctggccgagg ccgctgatgg cccggcggcg caacgcctcg cggacctgtg tcgcgaacac
    19261 gggctggcgc tgtgttacgg ctatgccgag cgtcacccgg ctggcggcaa accgtacaac
    19321 tcggcgcagt tgatcgacgc ccaggggcag cgcctggcca actaccgcaa gacgcatttg
    19381 ttcggggcga tggagcgcga gctgtttacc ccgggcgatg ggttcgaggt gccggtgctg
    19441 ctgaatggtt ggcgcgtggc gttgctgatc tgcttcgacg tggagttccc cgaggcggtg
    19501 cgcagccacg ccctggttgg tgccgagctg gtactgattc ccaccgcgct gacgcccgaa
    19561 taccgcgttg tgccggaact gattgtgccg atccgggcgg tggaacatca gctgttcgtc
    19621 gcctactgca accacagcgg ggtggagaac ggcctggcct ttctcggtgg ctcttgcgtg
    19681 gccgcgccgg atggcgagcg cttcggtgcc ttggccggtg gtgaaggtgt gctggtggtc
    19741 gacctggacc gcagctagcg tgacagctgg cgcgagcgtt ttccgtacct cgaagggcgg
    19801 tgcccggcac tctacggtga gctgctcaag ggcgaaaggt tgcgcccgga cacctcattg
    19861 ggacgagatg ccgggcgctc acacgctgtt tagaacgcca agccgatctt caatcccact
    19921 tcttcctgct tgagcttggt gcgctcggcc accgcgctgc tgctgtcgag ctcgtagcgg
    19981 gtctggctgt agtacaggct ggtgctgaca ggcaggttgt tgatgccctt gttccatagc
    20041 accgacaatt cgccgtaggg gttgaccttg tccttcaggt cgacggtgtc actctcgtca
    20101 ccgatcttca gtttgctttc ggcgttgatc gagtagcgcg cgcccaactc caggcgaact
    20161 gtgtagtcgg gtgtcaggta gttgtagccc agacctgcct tggcgaacgg cgagcgggtg
    20221 gtcagcttga cgtcttcttc gaacagaccg acctgcacgt catcctgttc ggtacgtccc
    20281 cagtcgtaac cacccccgac cagtacgtcg atgtagttgt tggtgctcaa ggcggcacgc
    20341 aggccgaggt cgaggtcggc acgggcggtc ttgtattctg tatcgtcttt ttcgcggtac
    20401 tggccttcga taccgatctg gtagatcggg ccttcctggc cggtcatctt gttgccgaag
    20461 ttatagaaca gaccgcccag gttgaggcgt tccttatcgc tgtcgtcgtc gacagtgagt
    20521 ttgtattgtt tgtgcgagcc aatcacgcca agcgtggaga tcggatcggt gatgctctcg
    20581 gcatgagctg ccgtgacagc tgcgccgctc aggctgaggc cgatgaggag acgggagggt
    20641 aggtaagtgc gtggcatgag tgggtccttg ctacaagatc aaaaagacat gacgcaccgc
    20701 aatggtgcgg cgcgtcagcg ttctcttgta tggactggtg gtgttgcggc gctattcgac
    20761 taaaccgacg aacggaagga accctttttc ctgcagcctt gaggcccatt ttagaggcct
    20821 tagcacccga tgaatggcct cagtcgagtt tcagcacctt ggccagcacg atcttcgggc
    20881 ctttcatctt cttgatgatg atgcgcaggc catctacttc aagcacttcc tcttcttcgg
    20941 gcatgcgctt gagcgtgtcg taaatcagtc cggcaagggt ttccgcctct acgtgatcga
    21001 ggtcgacact gagcaggcgt tcgaccttgg ccagtggcgt atcgccgcgt accagcagct
    21061 tgcctggctg ataggcaaga atgccgcgct cggccttgcg atgttcgtcc tgaatatcgc
    21121 caaccagggc ttccagtaca tcctccatgg tcaggtagcc gatgaccttg ccgtcggctt
    21181 cctcgaccag ggcgaaatgt gagccgccgg tgcggaactg ctccagcaag gtggacagcg
    21241 gcatatgccg gcctacccgt tcgaccgggt gcatcagctc ggcgagtttt agcgccgagg
    21301 gcagcatttc cagcagcgat aggtgcagta gcaagtcctt gatatgcagc acgccgacat
    21361 actcgccgct ggtgtcgtcg tagatcgggt agcggctgta cttgtgccgg cggaacacgc
    21421 tgaacacttc atccagggcg gccgtcagtg gcagggacac caggtcctcg cgggagttgg
    21481 cccagtccac cacttccagc tcgcccatct ccactgccga ggccagcaca cgcatatcct
    21541 ggtcgctggg gtcgctagcg cggctggagt ggaggatcag tttgagttcg tcgcggctgt
    21601 agtggtgctc atggtgcggc cccggctcgc cctggccggc cacgcgcaga atggcgttgg
    21661 cgctggcgtt gagcaggaag atcgccgggt acatggccca gtagaacagg tacagcggcg
    21721 cggccgtcca caacgacagc agctcgggtt tacggatggc ccaggacttg ggggccaact
    21781 cgccgaccac gatatgcagg taggaaatga taaagaacgc ggtaaagaag gcgatgccat
    21841 gtatcagctt gggcgattcg ataccgatgc ccgccagcag cggttcgagc aggtgagcaa
    21901 aggccggttc gccgacccag ccaaggccga gggaggctag ggtgataccc agctggcagg
    21961 ccgagaggta agcgtcgagc tggttgtgta cggtgcgcag gatatgcccg cgccagccat
    22021 tcttggcggc cagggcatcg accttggtgg cgcgtagctt gacgatggcg aactcggcgg
    22081 caacgaagaa gccgttgagc agcaccagaa acagagcgaa gaggacgagg ccgaaatcgg
    22141 cgaaataatt gctggcgacg taactcgtgg aggggtccat agagggttcg ggtagtgaat
    22201 gaccgctaaa gggtgggggc tgacagccgg ctttgcaagt ggggcgcggt tactggccgc
    22261 gccgtactac ctgcagggtg gggaagtggc aggtgaaggt gctgcccttg cccagcttgc
    22321 tgacgatatc cagccgcgcg cggtggcgca gcagcacatg cttgacgatg gccaggccca
    22381 agccagtacc gccggtattg ctggcgcggc tggaatcgac ccggtagaag cgctcggtca
    22441 ggcgcggcaa atgcttggcc tcgatgccca ggccgctgtc ctgcacgcta aggtgcgcgc
    22501 cctgttcatc gctccaccag cgaatgcgaa tctcgccttc atccggggtg tatttcacgg
    22561 cgttgaacac caggttggaa aaggcgctgc gcagctctgt ctcgctgccc ttgagcttga
    22621 ggtgcgggtc cgcctccagg ctgatacggt gatgacgctc gctggacagt gcctgggcat
    22681 cgttcttgat gctcagcagc agcaggtcga cggctaccgg ctggttgtcc gaggggtagt
    22741 cggtggcttc cagcttggcc agcaagagca gatcgttgag caggttctgc atgcgcccgc
    22801 cttgctgctg catctgctgc agggcacgca accagcgcgg attaacgttc tcgacgttgt
    22861 ccagcaaggt ttccaggtag ccggcgatca cggtcagcgg ggtgcgcaac tcgtgggaga
    22921 cattggcaac gaagtcctta cgcatctgct ccagctgata caggcgggtg atgtcgcgca
    22981 ctagcatcag gtgttcacga ttgccgtact gggtgatgtg gaattgcagg cgcaggcggt
    23041 cgctgaccgg tgacggcagc tccagcggct cgttgtaatt gtggttctcg aaatattcga
    23101 taaagcgcgg gtcacgcacc aggttggtga tctgctggcc gctgtcctgc ggggttttca
    23161 gacccaggag tttctcggcg gcgatgttcc accactccag gttgccgtcg cggtcgagca
    23221 tgatcaccgc atctttcagc gcggcggtgg actcctgcac gcggtcaatt accgcctgca
    23281 ggcgcccgcg gactttctgg tcgcggcgct gcaggtggta gatgctgtcg aatacttcgc
    23341 cccacaggcc gtagccatcc ggtggcgctt cgtcaccctg gcgggtgcgc agccatttgt
    23401 gcaggcgcag cagttgctgc aggtgccagc cgaggtagcc ggccaggccg agcagcaggg
    23461 tccaggcgta ttcgccggta atcagcccga gcagcaggca gacaccgacc agtagcaaca
    23521 agcgacgtat cagggggccg cgccagtctt gattcaattc gagctcatcc gccaatcaat
    23581 cctttatgca aacccatggt gggcctgcag gccatcctaa ccgtcttagc tcttggtgga
    23641 aaaacgatag ccggtaccac gcacggtttg caccaggttc tcgtagacct cgccgagtgc
    23701 tttacgcagg cggcggatat gcacgtcgac ggtgcgctcc tcgacataca cgttgccgcc
    23761 ccagacctga tcaagcagct ggccacgggt gtaggcgcgc tcctggtggg tcataaagaa
    23821 ttgcagcagg cggtattcgg tggggcccat ctcggccggt ttgccgtcga tggtgacgcg
    23881 gtggctgact gggtcgagca gcaggccgcc gacttcgatc ggcccttcgc tgtcggtcgg
    23941 gccagcgcgg cgcagcacgg ccttgaggcg ggcgaccagt tcgcgcgggg agaacggctt
    24001 ggtgatgtag tcgtcggcac cgacttccag gccctggatc ttgttgtcct cttcgccctt
    24061 ggcggtgagc atgatgatcg ggatgtcgcc ggtcagctca tcgcgcttga ggcgtcgcgc
    24121 cagttcgata ccgctggtgc cggggagcat ccagtcgagc aggatcagat cgggtttgcg
    24181 gtcgacgatg atggcgtgcg cctgctgggt attctccgct tccaggcact cgtagccggc
    24241 catttccagg gccacggcga tcatttcacg gatcggtgct tcgtcatcga cgatcaggat
    24301 gttcttgcca accatgggtc aagcctcggg tcatctcgct gtaatgtgct gcattagata
    24361 acggaattat tgcagcgata tgacaggcgc gcggcaaagc ttctttctag ccgcgcaagg
    24421 cgtagtccag taccaagccg ataaatatcg ccaaacccgc ccaatggttg tgcaggaagg
    24481 cattgaaaca ggccatcggc ttgcggttgc gggttttgtg aaactcccag gcaaagcatc
    24541 cggccgccgc cagcaggccg aggtggaagt tcaggcccag ctcgaagcgc gccgccgcca
    24601 gcagcagaca gagcagcgcc agaccctgga gaatgacaat gatcaggcgg tcggcatcgc
    24661 caaacagaat ggcggttgag cgcacgccga tcttcaggtc atcctcgcgg tcggccatgg
    24721 cgtagtaggt gtcgtaggcc accgtccaca gcaggttggc gatatacagc agccaggctt
    24781 ccggcggcag agtgccggtc tcggcggtaa acgccattgg catgccccag gaaaacgccg
    24841 cgccgagcac cacttgcggg tagaaggtgt agcgcttcat aaaggggtag caggcggcca
    24901 gagccaggcc gccaaacgac agccagatgg tcgtggcgtt ggtaaacagc accagcacga
    24961 agctggcggc taccagtacg gtgaacagaa tcagcgcttc acgcggctgg atcttgccac
    25021 tggccagcgg gcgggccttg gtgcggctga cgtggccgtc gaagttgcgg tcggcgtaat
    25081 cgttgatcac gcagccggcg gcgcgcatca aaaatacgcc gacgatgaag ataaacaggt
    25141 ttttcgcgct cggtacgccc tcggccgcca cccacaaggc ccacagcgtt ggccacagca
    25201 gcaggtaaat gccgatgggt ttgtccaggc gcatcagctg gataaagtcc caggtgcgcg
    25261 ggtgcaggcg gttgagtgaa tgcagcaggc gggtatacat cgagcgcgtc tccatgcgag
    25321 gtgcggctga ttatacggag cgcggcgcgc gatggggcgt aggtgatggg tatcgctaag
    25381 ctcaacctac gtggcccgcc ccatcctacg gctcgatggc ggccgtggcc cagaattcgg
    25441 gcagaaatac ctctgcgacc aaaaccccca gtgcaccacg gctaaagcac gagcgacgtg
    25501 cccacaggcg ctcgctgcag gcctctgctg gcagccactg cgacgggtag cgacacacct
    25561 gcaattcacc acgatcgaag gcgcggtcgc tgaacagcaa ttcacccagc gagcggctac
    25621 cgagttgatc cagcgtcaag ctggagcctt ccagtacgct gcgcgccgcc acgctgcggg
    25681 cgaataccca gggccggccg tgaccgcgta ggtacacctc gcgcacccag ccctggctgc
    25741 cgttgaccac gcccagcgcg ctgcattcat cgttgcgcag gcgctgccaa ccttcctgca
    25801 gcggagtgac gctgaagcca tcggccgaca gggcggtcag ccgccgcgtc agcgaatcct
    25861 cattgaacag ccagtcacgc accgcggcag tgggcgcagg gtgcagttgg ctggcagtca
    25921 gccagcgcgg cggataggcg agggtggggt ggggcacgag caggtttcac cgtaagcaga
    25981 ggcgcgcgag tctaacatgg cccttgcgcc cggacaggtg gtgccgggcg cgccgcttgg
    26041 ccgctacgcc gagcagaacc cgactttggt tggtctcgat gacaggctat tcatcgccct
    26101 gcggcttggc gcacctgcac atatacggta gtttccccgg cgcaatagcc gtgtgatccg
    26161 cctgaggtag ttatgaagaa gtggcaatgt gtggtctgtg gactgattta cgacgagagc
    26221 caaggctggc ctgatgaagg catcgccgcc ggcacgctct ggcaggatgt cccggaagac
    26281 tggctgtgcc ctgactgtgg cgtgggtaag cttgatttcg aaatgatcga aatcggttga
    26341 gtccaggctg tgacaagcgg cgacccaagg gtcgccgttt tgtttagcga gtgcgcatac
    26401 ttaacacccc gaacaagctg gataagcggc gaggtggtta tgcgcaagtg gcagtgtgtg
    26461 gtgtgcggtt ttatctatga cgaagccctc ggcctgcccg atgaaggcat cgcccctggc
    26521 acggcctggg aagatattcc cgccgattgg ctgtgccccg attgcggcgt cggcaagctg
    26581 gatttcgaga tgatcgaaat cgcctgagcc gcgtcagccc tgaaatctat tacggccgcc
    26641 gcagtgcgcg gcctgtgtga ggaatgagca tgagtgcacc tgtcgtcatc gttggcaccg
    26701 gcctggccgg ctacaaccta gcccgtgagt tccgcaagct ggacagcgaa acaccgctgc
    26761 tgctgatcac tgccgacgac ggtcgctcct attccaaacc gatgctgtcc accggttttg
    26821 ccaagaacaa ggatgccgat ggcctgagca tggctgagcc gggtgccatg gccgaccagc
    26881 tcaaagcgga agtgcgcacc catacccgca tcagtggcat cgatcccggt cataagcagc
    26941 tgtggatcgg cgaagaggct gtgccgtacc gcgatctggt gctggcctgg ggcgccgagg
    27001 tgattcgtgt gccggtgcaa ggtgatgccg atgatgcaat cttctcgatc aatgatctgg
    27061 aagattacgc gcgctttcgc actgcagccg ccggcaagcg gcgcgtcctg ctgctgggcg
    27121 ccgggctgat cggttgtgag tttgccaatg acctgagcct gggcggctat caggtcgagc
    27181 tggtggcgcc ctgcgagcag gtaatgccgg gcttgttgca tccggctgcc gccgcggcgg
    27241 tgcaggctgg cctggagggg ctgggcgcgc gtttccacct cggtccggtg ctggccagcc
    27301 tgaaccacca gggcgatgcc ctgcaggcgc acttgtctga tggcacgctg attgagtgcg
    27361 atctggtgct ctcagctgtg ggtctgcgtc cgcgtatcga cctggcggct gctgccgggc
    27421 tggcggtcaa tcgtggggtg gtggtcgacc gccagctgcg cacctctcac gccaatatct
    27481 acgccctggg cgactgcgcc gaggtcgatg ggatcaacct gctctacgtc atgccgctga
    27541 tgaccagcgc ccgtgcactg gcgcaaaccc tggccggcat cccaacggcg gtgagttacg
    27601 ggccgatgcc tgtcacggta aaaaccccgg tctgcccgct ggtggtatcg ccgccaccgc
    27661 gaggcctgca aggcgagtgg agcgtggagg gtagcggcgc cgatatcaag gcgctgtgcc
    27721 atgacagcag cggcgcgctg ctcggctatg ccctcaccgg tacggccgtg caggaaaaat
    27781 tggctctgaa taagctgctg cctgcgcttt tggcctgaat ggcggccgtt ctgtcggata
    27841 tgccacaaaa ttgcctggtt taactgtcgg acaagactgg cgcagggccc aatggcatgc
    27901 cattctccct cagtctgccg cagcctagag ctgctgcggc gccttggtat ctgctcgcgc
    27961 agagcagccc ggacacaaca acaaaaaacc gtaaagaggc atttatgcgt aaaccggaac
    28021 tcgccgccgc tatcgccgat aaggcggatc tgactaaaga tcaggcaaac cgtgtactta
    28081 acgcggtact cgaagaaatc accaacgcac tgaaccgcaa ggacagcgtc actttggtgg
    28141 gcttcggtac tttcgtgcaa cgccaccgcg gtgctcgcac cggtaaaaac ccgcaaaccg
    28201 gcgagccggt aaagatcaag gccagcaaca ccgtcgcctt caaaccgggc aagtccctga
    28261 aagacgcggt caactgagtc ctcaggcccg gggcctttgg ctccgggctg aaccaccccg
    28321 gctagcgtgt tcacgccctt ctttcagccg gatgtagtcc ccgccgcaaa ccgctacaat
    28381 gcgccgcctt ttccctaccc tttgcgaggc ctcgcgcatg aaattccgtt ttcttctgtg
    28441 gatgctgggc cgtctgatgg ccaaggccag ccgcgaaaac ccggcattcc aacagcaact
    28501 gctggaccgc gatatggtct tccagctgca caccctggat ggcaaagtcg cccgtcactt
    28561 catcgtcaaa gaccagcgca tcaccagcaa gcgcggcccg gctactgagc cagccttcgc
    28621 cctcggcttc aaggacgccg cctatggttt cgccaccatg agcgcgaaaa acaagcagct
    28681 ggcgtttatg cagggcattc agaacaagga catccagatc cagggcaacc cggcgctggt
    28741 gatctggttc cagggcctga ccaaatacct gatgccgaag aagaaagacg ccgccaaaaa
    28801 agctgcctga tccgcagctg tgcgcgccgg ggctggcctg cgcgcaccgc attcgctagg
    28861 ctgggtatag ccctagtgag agtttgccca tgcgtttgct gctgagtgcc ctgctatttt
    28921 ccacctccct tgctcatgcc gcgcccccca aagccgaagc ctttgcgccg ttattcgagc
    28981 tggcgggtat ccgcttgctg tgcgaacaga gcgcgccgct ggtgcagcgc ggcctgactg
    29041 ataaacagca ggcgcagttg gccaaggtgt ttgccgccga tggcctgtgc ctggacttgg
    29101 ccaagcggct gaccagcaag ctcgatcaag cccagctgca gcacgcccag agcttgctgg
    29161 aaagcccgct ggcgcagggc tttaccgccg cagaacggtc ggttggtgaa ggcggcgccg
    29221 aaggcctggc ggcctatcgc aaacaactgg aagagcgtcc gccgcgtgag gagcggctgg
    29281 ctctggtacg gcgtctggat gccgccgcgc acaccaccac cctggccacc ctgttgcgct
    29341 atgaagtcgg caagactcag gcgttgctgg cgctgcagag ccgtggcgaa agcatcgacg
    29401 agaaagccat gagcagccaa accaaggctc aggaggacgc tttgcgcgcc tccagctctg
    29461 aggcggtgga gtcttttatg ctttatgcct accggcagat acccagcgaa aaactggccg
    29521 aatatgccgc cctgtatgag cagccagccg tagctcagtt gcttggtagt agcgtgcagg
    29581 tgctgccgga gctgtttgcc gcacggcgtg cgcagctgaa gaagtagtta gtcgactgaa
    29641 acgacaaagg ggcccgtagg cccctttgtg ctttctgcgc ttttaatgtg cgggatggaa
    29701 ttgcgccgcc agttctcgca gtgcgacttc agcggccagc accttgctca ccgcctcttc
    29761 ggctttctcc cgactaatgc cgaggttgtc gaacaggctt tgcggaatct cactctgctg
    29821 gccggcgccg atgccgcgat tgcgcagcag gctgaccgac aggcacacca gattcgggta
    29881 ggtggcgtgg gggccggtgt actcaggatc gtgctggaag cgcagggcgc tggccagttc
    29941 ttccggcata tcccagtaac gcatcagcca ggcgccgatc tgttcgcggc tgataccgag
    30001 cagatgttgc tcgatatagc tgtgcgacag gtgcgggttg acctccagat gccggcagat
    30061 cagcgagaag tgcggcggaa acacatgcgc cagcaccaga taaccgaagt tatgcagcag
    30121 gccggcgagg taggtcaggc cggcttccgg gcgctgtgcg cgtgggatgg cgcgggtcag
    30181 accctcaatc accgcagcgc tgtagatcgc ctgttgccag tacggcgtgg cctgttgcgg
    30241 gtggtccttg ggcaggctca gggtcttgcc tagggccagg ccgagggcca ggttgatcac
    30301 cagatcaaag cccagcacac ggacgatggc gtcttccacc gagcggatct tgccgggcgc
    30361 ggcatagaag ggcgaggcgg cccagctgac cacctgagcg gccagcgccg ggtcggtttc
    30421 gaccacgccg gtgatgtcat cgacggtggc atccgggtcg acgcgcagct tgatgatctt
    30481 ctgcgcggtt tccggcagtg gaggaatctc gatggtttct tccaggcgtt gctggatgcg
    30541 ccgtgcggta aaggcctgca ccgccagggt gatctcggcg cggtcgtcgt ccgggcgatc
    30601 gaggttcagg cggatattgc tcagcggctc gccgaagcgt gcggcgctgg ccttgctcaa
    30661 caaggtcttg aaggcttcgg tgctcagctc cagcaatacg ccgcgctggc cggaatcgat
    30721 atacaggctg ggcacctgca gcaggcgctc gtcatatagg cagggcgagc tggtcagcgg
    30781 cggcaggccg ggcagcactt tcaggctgtg cttgccgagc atgcgttcca ggcgctctgg
    30841 cttgacggcg gtcagctggc ggccggtcag ttcggtcagg cgattgaggt cgagcagctg
    30901 gctctgtggg tagagcacca gtagcgcgcc gaccgcgtca tcgagcaata ccgcttgcag
    30961 gcgctgagcg gccggcagac cgggctggtc ggggcagatt tggtagggca ggccgagctt
    31021 ttccagcaat tgcaaaatca ccgctggcgg gcgcggagcg gtgtcggtag caagggcaag
    31081 ttcagtcatg gcggtatccg gctgaggcag caatttcacg agtataaaca gcgggctggt
    31141 gaatcggcgg cgcaggcgac gggtgcactg tgcgctggtt cacacttgcc cgtattgttg
    31201 gccatgccgc aaccagcgtt caagtagcgg gctgacatgc tgtggccagt gttccagcag
    31261 cgcctgcgcg gcgtcgcgta cggctggtag caggtcggca tcgcgcatca ggtcggcgac
    31321 cttgaactgc agcaaaccgg tctggcgggt gccgagcatc tcgccggggc cacgcagttc
    31381 caggtccttt tcggcgataa cgaaaccatc gcaggtctcg cgcatgattg ccaggcgctc
    31441 gcggcccaac tgcgacaacg gcggatgata gagcagcagg caatgactgg ccgcgctgcc
    31501 acgaccgacc cggccgcgca gctggtgcag ctgggccagg cccaggcgtt ccgggttttc
    31561 gatgatcatc aggctggcat tgggcacatc gacgccgact tcgatcaccg tggtggccac
    31621 cagcagttgc agctggccct gtttgaactg gtccattacc gcggcttttt ccgccggttt
    31681 catgcgcccg tgaatcaagc cgacattcag cccgccgagc gctgccgata aatcctcgaa
    31741 ggtggtttcc gcggcctggc aggtcagctc ttcggattcc tcgatcaggg tgcacaccca
    31801 ataagcctga cgcccttcat tgcaggccag gcgaacgcgc tcgaccactt ccaggcggcg
    31861 gctgtcggcg attaccaggg tgttcactgg ggtacggccg ggcggcagct cgtcgaggat
    31921 ggaggtgtcg aggtcggcgt aggcgctcat cgccagggtt cgtgggatcg gcgtcgcagt
    31981 catgatcaat tggtgcggac acatgcgccc gccgatgcct ttctgccgca gggccaggcg
    32041 ctgttgcacg ccgaagcggt gctgctcatc gataatcacc agcgcgagtt tttggaactg
    32101 cacttcatcc tgaaacagcg cgtgggtgcc aaccaccatg ggcgcgcccg cagcgatctg
    32161 ctccagggcg ctggcgcggg ccttgccctt gagcttgcct gccagccagg cgacttcaag
    32221 acccagcggc gcgagccatt tgctgaagtt gataaagtgc tgttcagcga gaatctcggt
    32281 aggggccatc agcgccacct ggtaaccggc ttccagtgct tgcagtgcgg caaaggcggc
    32341 gaccacggtc ttgccggcgc cgacatcgcc ctgcaccagg cgcaacatcg gctcgctctg
    32401 gctgaggtcg taggcgattt ccgcgccgac ccgctgttgc gcgccggtgg gggtaaagcc
    32461 caggttggcc aggtattgct gcggcaggct tttcgccagg ggcagcgctg gagctagctg
    32521 cgcgcggacg ctctcgcgca agcgttgcag cgacagttga tgggtcagta attcttcgaa
    32581 ggccaggcgg tgctgggccc agtggcggcc ttcggcgagt tcttccaggt cggcatccgg
    32641 cggcggtcga tgcagatagc ggatcgcctg atcgagcggg ctgagctggt aatcgtcagc
    32701 caactcgcgc ggcagccaat ccggcaggct gtgcgggccc aagcgcgcca gggcctgctc
    32761 actaagctga cgcaggcgtt gctgggtcag gccttcggtg gtcgggtaga tcggcgtcag
    32821 ggtctgttcc acggcaatcg gttcgctgcc ggacagcgcg cggtattccg ggtggtagat
    32881 ctccaggccc gacgagccag ggcgcacttc gccgtagcaa cgcaccaggg tgccgcgctt
    32941 gaggccttct ttttgcgcct ggctgaagtg gtagaagcgc aaggagaggg tgccgctgcc
    33001 gtcctgcagg cgtaccagca ggctacggcg tcgacccatg accacatcgg cgccggcgac
    33061 tatgccttcc accacggcgt cctgaccggg gcgcagcgcg ccaatcggca cgatgcgggt
    33121 gcggtcctga tagcgcagcg gcaggtggaa cagcacatcc tgcagggttt ccaggccaac
    33181 cttggcgagc ttttcggcca gggctgcacc cacgcctttc agcgcggtga cagaaaccgc
    33241 agccagctcg gtcatggcct gtcttagctg ctcttgggca gtggtttggc caccgagcac
    33301 agacggatcg agtcggtaag gacttcgatg gcattcggcc gcgggaagct ggcgcgccag
    33361 gcgatggcca cggtgcggaa cggcgcgggc gcgctaagtg ggcgcacttc gatcacgccg
    33421 ggggcatagt ggtggctgtc gaccgcagag aacggcagga tcgacacgcc taggcccgag
    33481 gcgaccatat ggcgaatggt ttccagcgag ctggattcca ccgtggtgtg cttggcgctg
    33541 tcttcgccgc ccttgcgcag ggtcgggcag gcttccagca cctgatcacg gaagcagtga
    33601 ccttcaccga gcagcagcag gctcttgtca ttcagcagct tgctgtcgat ggtcttcatc
    33661 gcagcccaag ggtgaccggc tggcagcagg gcgtagaacg gctcgtcgta cagcggcttg
    33721 gtcagcacat cagcttcatg gaatggcagg gcgatgatga tcgcgtccag ctcgccgttg
    33781 cgcagcttgt cgcgcagcac gtgggtgaag ttttcttcga tatacagcgg catctgcggg
    33841 gcgacgcggt gcagctgtgg aatcagatgg gggaacagat aagggccgac ggtatagatc
    33901 gcgccgattt tcagtggcgc ggccatctga ttcttgccgg cttgggccag ctcgcggatg
    33961 ccttgggcct gctccagcac cttctgcgcc tgggtaacga tgccttcacc gaccggagtc
    34021 aggcgcactg cgctcttgct gcgttcgaag atcagcacac ccagctcatc ttcgagtttc
    34081 ttcaccccca ccgacagcgt cggctggctg acatgacaac gctcggcagc gcggccgaag
    34141 tgctgttcct gagcgagggt gactatgtag cgcagttcgg tgagggtcat agtctttatc
    34201 cattgagatg cagccaagca tagcggctgt attcaatgga accaactgtt gataccgctc
    34261 tgcagcgctg gctcaagccg caaaacggtt ttggcgattg cttacgcgtt tatgtcgctc
    34321 tgtgaacata aaataattgg ttactaattc gtagcacgca tagaattgcg gccatacttg
    34381 gcgggcagct ctgtttgggc tgcgcgcaat aaataattat agaagccgtt ccttatcaag
    34441 gagtaccggc atgcctgatg cggtaacgac catatcccat aactgggctt ttgctgtttt
    34501 tctgcttggg gtctgcggcc tcattgcatt catgcttggt ctgtccagcc tgctgggtag
    34561 caaagcctgg ggtcgcagca agaatgagcc ttttgagtcc ggcatgttgc ccgtcggcag
    34621 cgcgcgtctg cgcctgtcag ccaaattcta tctggtcgcg atgctcttcg tgatcttcga
    34681 cgttgaagcc ctctttctct tcgcttgggc agtttcggtg cgcgaaagcg gctgggctgg
    34741 gctgatcgaa gctacagtct ttatagcaat tctgttggca ggtcttgtct acctttggcg
    34801 gatcggggcg ctcgattggg cacctcaggg ccgtcgcgag cggcaggcga agctaaaaca
    34861 atgaggcttt ggcgatgcac tacaaactta ctcggatcga tccggatgcg ccgaatgagc
    34921 gctatccggt cggcgagcgg gagactgttt ccgacccgct gctagatgat caagtccaca
    34981 agaacatcta catgggcaaa cttgccgatg ttctgaatgg cgcagtgaac tgggggcgca
    35041 agaactccct atggccgtat aacttcggct tgtcctgctg ctatgtggaa atgaccaccg
    35101 cctttaccgc gccgcacgac gtggcccggt ttggtgcgga agtgattcga gcctccccac
    35161 gccaggcgga cttcatggtc atcgccggca cctgcttcat caagatggct ccggtcatcc
    35221 agcgcctgta cgagcagatg ctggaaccca agtgggtgat ctcaatgggc tcctgcgcca
    35281 attccggcgg catgtacgac atctactccg tggtccaggg agtcgacaag ttcctgccgg
    35341 tcgacgtcta tattcccggt tgcccgcccc gtcctgaggc atttctgcag ggcttgatgc
    35401 tgctgcagga atccatcggt caggagcgcc ggccgttgtc ctgggtagtc ggtgatcagg
    35461 gtgtttaccg tgccgacatg ccttcgcaga aagaacagcg tcgcgagcag cgaatcgccg
    35521 tgaccaatct gcgtagcccc gacgaagtgt gagcctgggc tcgattgagc ccgcctactg
    35581 acgccgttga cctatagcga ccgagaccat gactgcagac agcattctgt ccataccgcc
    35641 gtacaaggct gacgaccaag atgtggtcgt cgaactgaat tcccgttttg gcgctgacgc
    35701 ctttaccgca cagagcaccc gtaccggcat gccggtgctg tgggtcgctc gcgagcggct
    35761 ggtcgaaatc ctgaccttcc tgcgcaacct gcctcgcccc tacgtcatgc tgtatgacct
    35821 gcatggcgtc gacgaacgcc tgcgcaccca gcgtcgtggc ctgccgggtg cggatttcac
    35881 cgtgttctat cacctgatgt cgctggagcg gaacagcgac atcatgctca aagttgcctt
    35941 gagcgagcgc gacctcaatg tgcccagcgt caccagcatc tggcccaatg ccaactggta
    36001 tgagcgcgaa gtctgggacc tctacggcat ccatttcagc ggccacccgc acctaacgcg
    36061 gatcatgatg ccgccgacct gggaaggcca cccgctgcgc aaggattacc cggcgcgcgc
    36121 caccgagttc gatccgttca gcctgaccct ggccaaacag cagctggaag aagaagctgc
    36181 gcgcttcaaa ccggaagact ggggcatgaa gcgtggcagt gagcacgagg actatatgtt
    36241 cctcaacctc ggccccaatc acccgtcggc ccacggtgct ttccgcatca tcctgcagct
    36301 cgacggtgaa gagatcgtcg actgcgtgcc ggaagtcggt taccaccacc gcggcgccga
    36361 gaagatggcc gaacggcaga gctggcacag ctttattccc tacaccgacc gcatcgatta
    36421 cctcggcggg gtgatgaaca acctgccgta cgtgctggcc gtggaaaaac tcgcgggcat
    36481 caccgtgcca cagcgtgtcg acgtgattcg catcatgatg gctgagttct tccgcatcac
    36541 cagtcacctg ctattcctcg gcacctatat ccaggacgtc ggcgcgatga ccccggtgtt
    36601 ctttaccttt accgaccgcc agcgcgccta caaggtgatc gaggccatca ccggcttccg
    36661 cctgcacacg gcctggtacc gcattggtgg cgtcgctcac gacctgccgc gtggctggga
    36721 tcgtctggtc aaggagttcg ttgactggct gcccaagcgc ctcgacgagt acgagaaagc
    36781 cgcgctgcag aacagcatcc tcaaggcgcg gaccatcggt gtggcgcagt acaacaccaa
    36841 ggaagcgctg gagtggggca ccacaggtgc tggcttgcgc gcgactggct gcgacttcga
    36901 cctgcgcaag gcgcggccgt attccggtta cgagaacttc gagttcgaag tgccgctggc
    36961 cgccaatggc gatgcctacg accgctgcat ggtgcgtgtg gaagagatgc gccagagcat
    37021 caagatcatc gaccagtgcc tgcgcaatat gccggaaggt ccgtacaagg ccgatcaccc
    37081 gctgaccacg ccgccgccga aagagcgcac gctgcagcac atcgaaaccc tgatcaccca
    37141 cttcctgcag gtttcctggg ggccggtgat gccggccaac gagagcttcc agatgatcga
    37201 ggcgaccaag ggcatcaaca gctactacct gaccagcgac ggcagcacca tgagctaccg
    37261 cacgcgcatt cgtacgccaa gcttcccgca cctgcagcag atcccttcgg tgattcgcgg
    37321 cagtatggtc gcggacctga tcgcgtacct gggcagtatc gacttcgtta tggctgacgt
    37381 ggaccgctga ttatgagcac gctaatccag actgatcgtt tcatcctcag cgaagccgaa
    37441 catgcggcca tcgagcatga gatgaaccac tacgaagacc cgcgcgcggc gtcgatcgaa
    37501 gcgctgaaaa tcgtgcagaa agcccgtggc tgggtgccgg atggcgccgc cgatgcgatt
    37561 ggcgaaattc tcggcattcc cgccagcgac gtcgaaggtg tggccacctt ctatagtcag
    37621 attttccgtc agccggtggg ccgccatatc atccgcgtat gcgacagcat gacttgctat
    37681 gtcggtggcc atgagtcggt cgtcgctgaa atgcagaaac agctcggtat cggcctcggc
    37741 gagaccacca acgacagccg ctttaccctg ctgccggtgt gctgcctggg taactgcgac
    37801 aaggccccgg cgctgatgat cgacgacgat acctttggcg acgtgcaacc tgatggcgtc
    37861 gccaaactgc tggaggccta cgtatgagca tcaagacgct gacctccatc ggcccggcca
    37921 acaagatcgc ccgcagcgag gaaagccatc cgctgacctg gcgcctgcgt gacgacgccc
    37981 agccggtgtg gctggaggag taccagcaga agaacggcta cgccgctgcg cgcaaagcgc
    38041 tgagtgagat ggctcaggcc gatatcgtgc agacggtgaa ggactccggc cttaaaggtc
    38101 gtggcggtgc tggcttcccc accggggtga agtggggcct gatgcctgcc gacgaatcca
    38161 tgaacatccg ctacctgctg tgcaacgcgg atgagatgga accgaatacc tggaaggacc
    38221 gcatgctgat ggagcagctg ccgcacctgc tggtggaagg catgctgatt tccgcgcgtg
    38281 cgctcaagtc ttatcgcggc tacatcttcc tgcgcggcga gtacgtcgat gcggcggcga
    38341 atctgaatcg cgccattgag gaagccaagg ccgccggcct gctcggcaag aacattctcg
    38401 gcagcggttt cgatttcgaa ctgttcgttc acaccggtgc cggccgttat atctgcggtg
    38461 aagaaaccgc actgatcaac tcccttgaag gccgccgcgc caacccgcgc gccaagccgc
    38521 cgttccctgc cgccgtcggc gtgtggggca agccgacctg tgtaaacaac gtcgagactc
    38581 tgtgcaacgt gccggccatc gttggtaacg gtgtggattg gtacaagagc ctggcccgcc
    38641 ccgagagtga agaccacggc accaagctga tgggcttctc cggcaaggtt aagaaccccg
    38701 gcctttggga gctgcccttc ggcgtcagcg cccgcgagct tttcgaggat tacgccggtg
    38761 gcatgcgcga tggctatacc ctcaagtgct ggcaacccgg cggtgccggc accggcttcc
    38821 tgctccctga gcacctggat gcactgatgt atgccggcgg tatcggcaag gtcggcacgc
    38881 gcatgggcac cggcctggca ctggcggtgg acgacagcat cagcatggtt tcgctgttgc
    38941 gcaatatgga agagttcttc gcccgtgagt cctgcggctg gtgtacgccg tgccgcgatg
    39001 gcctgccgtg gagcgtgaag atcctccgcg ccctcgagcg caagcaaggc actcagcagg
    39061 acatcgacac gctgctcggc ctggtcaatt tccttggccc aggcaaaacc ttctgcgccc
    39121 atgcaccggg tgcggttgag ccgttgggca gtgcgatcaa gtatttccgt tcggagttcg
    39181 aggctggcgt ggccagctcg gcggctccgg cgacgacggg gcaattcgtc cccgcgtaaa
    39241 aaatggcgct ctggcggtca ctgacctggc cccgcagcac aacgtgattc cattagccaa
    39301 gcccgcttcg gtgggcaaag aagaaacctg aaccatggcc actatccacg tagacggcaa
    39361 agatttcgaa gtcgatgggg cggacaacct gctgcaggcc tgcctgtccc tcggtctcga
    39421 tatcccttac ttctgctggc acccggcgct cggtagcgtc ggcgcctgcc gccagtgcgc
    39481 ggtcaagcag tacaccgatg aaaacgatac gcgcgggcgc atcgtcatgt cgtgcatgac
    39541 gccggccacc gacaacacct ggatcagcat cgacgatgaa gaatccaagg cgttccgcgc
    39601 cagcgtcgtc gagtggctga tgaccaacca cccgcacgac tgcccggtgt gtgaggaggg
    39661 cggccattgc cacctgcagg acatgacggt gatgactggc cacaaccagc gccgttatcg
    39721 cttcaccaag cgtacccacc agaaccagca gctcggcccg ttcatttccc acgagatgaa
    39781 ccgctgcatc gcctgctacc gctgcgtgcg ttactacaag gattacgccg gcggcaccga
    39841 tctgggcgtg tatggcgcgc acgacaatgt gtacttcggt cgggtcgagg acggcgtgct
    39901 ggaaagcgag ttctccggca acctggtcga ggtctgcccg accggtgtgt tcaccgacaa
    39961 gacccactcc gagcgctaca accgcaagtg ggacatgcag tttgccccga gcatctgcca
    40021 tggctgcgcc agcggctgca acaccagccc cggcgagcgc tacggcgagc tgcgacgcat
    40081 cgaaaaccgt ttcaacggcg atgtgaacca gtacttcctc tgcgaccgtg gccgtttcgg
    40141 ttacggctac gtcaaccgca gcgatcgtcc gcgtcagccg ctgctggtca ataaagacgg
    40201 tggcgcaaag ctaggcatcg acgcggcgct ggacaaggcc gccgagctgc tcaagggcaa
    40261 acgcataatt ggcatcggct cgccgcgcgc cagcctggaa agcaacttcg ccctgcgcga
    40321 gctggtcggc gaggctaact actaccccgg tatcgctgcg ggcgagttgg acaatatccg
    40381 cctgatccgc gacatcctgc agaacggccc gctgccggtg ccgaccctgc gtgaagtcga
    40441 actgcacgac gcggtgttta tcctcggcga agacctcacc cagaccgccg cacgcctggc
    40501 cctggccctg cgtcagacgg tcaagggcaa ggccactgaa atcgccacct cgatgaaaat
    40561 tcaggactgg cacatggctg ccgtgcagaa cgtcgctcag gacgcgctgc atccgctgtt
    40621 tatcgccagc gtcaccgcta cacgcctgga tgatattgcc gaagcctgcg tgcatgctgc
    40681 accggctgac ttggcgcgta tcggtttcgc cgtggctcac gccatcgacc cgagcgcacc
    40741 ggcggttaac ggcctggatg cggatgtcgg tgagctggtg gagttgattg ccgaagcgct
    40801 gctagaagcc aagcgcccgc tgatcatctc tggcgcctct ctcggtgaga agagcctgat
    40861 cgaggccgcg gcgaatatcg ccagcgcgct gaagaatcgc gagaaaaacg cctccatcag
    40921 cctggtggta ccagaagcca acagcatggg cctggccctg ctgggtggcg agtccgttga
    40981 tgcggcgctg caggcgctga cgtccggcca ggccgatgcc gtgatcgtgt tggaaaacga
    41041 cctgtaccgc cgcgccgatg ctgcccaggt tgatgccgca ttggcggccg ccaagctggt
    41101 gattgtcgct gaccatcagc acaccgccac cagcgaaaaa gcccatctgc tgctgccggt
    41161 ggccagcttc gccgaaggcg atggcaccct ggtaagcttt gaaggccgcg cccagcgctt
    41221 cttccaggtg ttcgagccga gctactacga cgccaatatc ctgattcgcg aaggctggcg
    41281 ctggctgcat gcgctgcaca gcaccctgca gggcaagccg ctcgactgga ctcagctgga
    41341 tgcggtgacc gctgccgttg ctgctagcaa cagcttgctg gcacgcatcg gcgaagccgc
    41401 gcccaacgcc accttccgca tcaagggcct caagctggcc cgcgagccgc accgttacag
    41461 cggtcgcact gccatgcgcg ccaatatcag tgtgcatgag ccgcgccaac cgcaggacca
    41521 ggactcggcc ttctccttct ccatggaagg ctattccggc agcaaggaag accgtcagca
    41581 gattccattc gcctggtcgc cgggctggaa ctcgccgcaa gcctggaaca agttccagga
    41641 cgaagtcggc ggccatctgc gtgctggtga ccccggtgtg cgtctgctgg aagccaaagg
    41701 cgtggtattg ccctggttca gcgtaccggc cgcctttaac ccggctccgg gtaccttgca
    41761 ggccgtggcg ctgcatcacc tgttcggcag tgaggagacc tcggccggcg ccgcgccgct
    41821 gcaagagcga attcccgcac cttacgcgct gctggccaag gccgaagcgg atcgtctggg
    41881 ggtgaatgat ggcgcgctgc tgagcctgac agtcgctggg cagaccctgc gcctgccgct
    41941 gcagatcacc gaagagctgg ccatcggtgt ggtcggcttg ccggtgggct tggtgggcat
    42001 gccggcgctg atcgcaggta gcgtggtcac ccgcattgag gaggtcgcgc aatgagttgg
    42061 ctgacccccg aagtactgga cgtgctgatc gccgtactca aggccatcgt gattctgctg
    42121 gtggtggtga tctgcggcgc gctgctgagt tttgtcgagc gccgtctgct gggctggtgg
    42181 caggaccgtt acggccccaa ccgggtcggg ccgttcggca tgtttcagat cgccgccgac
    42241 atgatcaaga tgttcttcaa ggaggactgg acgccgccgt tcgccgacaa gttcatcttt
    42301 atcctcgcgc cgatgatcgc ctttgccgcc atgttgatgg cctttgcgat catcccgatc
    42361 accccgaact ggggcgtggc ggacctgaat atcggcatcc tgttcttctt cgccatggct
    42421 gggctgtcgg tgtatgccgt gctgttcgcc ggctgggcga gtaacaacaa gtttgccctg
    42481 ctcggcgcgc tgcgcgcctc ggcgcagacg gtgtcctacg aggtgtttct ggcgctggcg
    42541 ctgatgggca tcgtcgccca ggttggctcg ttcaacatgc gcgacatcgt cgattaccag
    42601 gcggaaaacc tctggttcat cattccgcag ttcttcggct tctgtacctt ctttatcgca
    42661 ggcgtggcgg tgactcaccg tcaccccttc gaccagccgg aagccgagca ggagctggcc
    42721 gacggttacc acatcgaata tgccgggatg aaatggggca tgttcttcgt cggtgaatac
    42781 atcggcatca tcaccatctc ggcgctgctg gtaaccctgt tcttcggcgg ttggcacggg
    42841 ccatttggca tcctgccgca gattccgttt ttctggtttg ccctgaaaac ctgcttcttc
    42901 atcatgatct tcatcctgct gcgcgcctcg attccgcggc cacggtatga ccaggtcatg
    42961 gccttcagct ggaagttctg cctgccgctg accctgatca acctgctggt gaccggcgca
//
LOCUS       DASRUM010000256        13738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1132365, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000256 DASRUM010000000
VERSION     DASRUM010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 13738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13738
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132365"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..2013)
                     /gene="dnaK"
                     /locus_tag="VFF22_17370"
     CDS             complement(97..2013)
                     /gene="dnaK"
                     /locus_tag="VFF22_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZX18624.1"
                     /translation="MGKIIGIDLGTTNSCVSVLENGVAKVIENAEGARTTPSIVAYAN
                     DGEILVGQSAKRQAVTNPHNTLYAVKRLIGRRFDEDVVQKDIQMVPYKIVKADNNDAW
                     VEVNGQKMAPPQISAEILKKMKKTAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAYGMDKAKGDHTVIVYDLGGGTFDVSVIEIAEVDGEHQFEV
                     LATNGDTFLGGEDFDIRLIDYLVDEFKKESGMNLKGDPLAMQRLKEAAEKAKIELSSS
                     QSTDVNLPYITADATGPKHLNVKISRAKLESLVEDLVQRTIEPCRIALKDAGIDIGAI
                     NDVILVGGQTRMPLVQKLVTDFFGKEARKDVNPDEAVAMGAAIQGAVLSGDVKDVLLL
                     DVSPLTLGIETMGGVMTALIEKNTTIPTKKSQVFSTADDNQGAVTIHVLQGERKQASG
                     NKSLGKFDLAEIPPAPRGVPQIEVTFDIDANGILHVGAKDKATGKTQSIVIKANSGLS
                     EEEIQQMVRDAEVNAEEDRKFEELATARNQGDQLVHATRKMITEAGDKATADEKTAIE
                     TALSALEVAIKGDDKAEIDAKMTALSEVTAPLAQKMYAEQPQPGAQAPGAEQAKDAGD
                     DVVDAEFEEVKDNK"
     gene            complement(2113..2682)
                     /gene="grpE"
                     /locus_tag="VFF22_17375"
     CDS             complement(2113..2682)
                     /gene="grpE"
                     /locus_tag="VFF22_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZX18625.1"
                     /translation="MADEQTLDTQNPDVQTAAEAAAGDDLVARVQALEEQLAAAQDQS
                     LRMAADLQNVRRRAEQDVEKAHKFALEKFANDLLPVVDSLERGLELSNPDDEAIKAVR
                     EGMELTLKLLGDTLKRHQLEAIDPHGAPFNAEHHQAMAMQESTSVEPNSVLKVFQKGY
                     MLNGRLLRPAMVVVSKAPVEAPPSIDEQA"
     gene            2839..4512
                     /gene="recN"
                     /locus_tag="VFF22_17380"
     CDS             2839..4512
                     /gene="recN"
                     /locus_tag="VFF22_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003177836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZX18626.1"
                     /translation="MLVHLSVHNYAIVEHLDLELDAGMSVISGETGAGKSIMLDALGL
                     TLGDRADSGVVRPGADKADILASFDLHDIPEARAWLAERDLDNDGPCILRRVITAEGR
                     SRGYINGSPCPQGDLKALGELLIDIHSQHEHQSLLKPDTHRRLLDEYAGSQELARQVQ
                     LAAQRWKQTKNELERVSSIGDEQRARHQLLSYQLEELDNLALGEDELDNLEQEHKNLT
                     NAESLLSACRLVIEQCSENDAGNVLSALTSSLNRLSAFQGQPGALSEATNLLASAQIQ
                     VEEAVGELNRFLDHFDADPERLRQMEERLDAIYTLARKHRIQPTELGAMQQQLFEELE
                     SLNADDQAAERLSDELAAFERHYQEKAAELSTLRNNAASQLASAVEQEMQTLGMPGGR
                     FNIKLSANSNSEPHPNGLEQLEFLVSANPGQPLKALAKVASGGELSRISLAIQVITAQ
                     TSRVPTLVFDEVDVGIGGPTAEVVGQLLRRLGERGQVLTVTHLPQVAAQGHQHLFVHK
                     VRESDATRTAVSKLSPAQRVEEIARMLGGVDLTEESLAHARQMVGSAQA"
     gene            complement(4578..4982)
                     /gene="fur"
                     /locus_tag="VFF22_17385"
     CDS             complement(4578..4982)
                     /gene="fur"
                     /locus_tag="VFF22_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HZX18627.1"
                     /translation="MVENNELRKAGLKVTLPRVKILQMLDHAGQRHMSAEDVYKALME
                     AGDDVGLATVYRVLTQFEAAGLVVRHNFDGGHAVFELADSGHHDHMVCVDSGDVIEFF
                     DAEIEKRQKEIVKEHGFELVDHNLVLYVRKKS"
     gene            5080..5598
                     /gene="bamE"
                     /locus_tag="VFF22_17390"
     CDS             5080..5598
                     /gene="bamE"
                     /locus_tag="VFF22_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017735535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HZX18628.1"
                     /translation="MQKTKILLTSLTLTGLLALAGCSFPGVYKIDIQQGNVVTQDMID
                     QLRPGMTRKQVRFIMGNPLLTDTFHANRWDYLYSIQPGGGKRLQERVSVNFNAEDQLV
                     GLSGDFMPGVSRDQAILGEDGGVTTPQTTQQPQVEEKPAPGSLLEQIQREVDDVKVVP
                     VPTPEPLESTPQ"
     gene            complement(5664..5984)
                     /locus_tag="VFF22_17395"
     CDS             complement(5664..5984)
                     /locus_tag="VFF22_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HZX18629.1"
                     /translation="MDKPNIVVEVVYALADKQKLLRLSLPYGTTVRQAAVQSGMQAHF
                     PDLDLHNSPLGIFGKAVSKPDERVLEEGERVEIYRPLIADPKEVRKQRAAKAAQAKAE
                     DGGN"
     gene            complement(5977..6411)
                     /locus_tag="VFF22_17400"
     CDS             complement(5977..6411)
                     /locus_tag="VFF22_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HZX18630.1"
                     /translation="MSTHIQRSALLPYPARALFDLVNDVASYPQFLPWCSASEVLESS
                     DTHMRASLAVAKGGLSQRFVTANTLVPGELIKLTLVEGPFTQLYGHWEFKALGDKACK
                     ISLDLTFDYAGPLVRATLGPLFNQATNTLVDAFCQRAKQLYG"
     gene            complement(6446..7849)
                     /locus_tag="VFF22_17405"
     CDS             complement(6446..7849)
                     /locus_tag="VFF22_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HZX18631.1"
                     /translation="MANDKVSIHGAWASRWVFILAATGSAVGLGNIWKFPYMTGMYGG
                     GAFVMMYLVCIFMVGLPIMLAETLIGRRGRQSPVNTLKTLAIESGASPKWSWAALVGM
                     VAALLILSFYSVVAGWSLDYILSMGGGQFAGVSAEGAGAAFGALTADPWRLTLWHTLF
                     MLLTGFVIARGVVAGLERSLRIMMPLLFVLLLVLLGYSMTTGHFMQGFHFLFDFQPDR
                     VLDGVLAAMGHAFFTLSVGVGAIMVYGSYMPKNASIGGTILTVGILDTLVALTAGMAL
                     FPIVFAGGLEPGAGPGLMFVTLPVAFGNIAFGQVMGTLFFILVLIAAWTSSISMLEPA
                     VAYLVERTKRSRVLVTSVLAFLCWVVGMGTVLSFNLGAEAKFFVFAEDGFHLFQWGAE
                     GGRNFFDTIDYLTSRILLPFGGLAFAIFAGWVLKREAVREELAMKSPLLFNVVYWLIR
                     VVAPIGVLIVFVAELSK"
     gene            8015..8497
                     /gene="smpB"
                     /locus_tag="VFF22_17410"
     CDS             8015..8497
                     /gene="smpB"
                     /locus_tag="VFF22_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HZX18632.1"
                     /translation="MAKQKKHPQGTIAQNKKAMHDYFVESRFEAGLVLAGWEVKSLRA
                     GKAQLVDSYVLLKNDEAWLMGCHITPLTAASTHVIADPTRTRKLLLNKRELDKLFGAV
                     QQKGYACVAMSIYWKAHLIKCEIALAKGKKDFDKRHTEKERDADREVQRAMRSKGKDE
                     "
     gene            complement(8561..9190)
                     /locus_tag="VFF22_17415"
     CDS             complement(8561..9190)
                     /locus_tag="VFF22_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017526162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HZX18633.1"
                     /translation="MYWTEFLAVALIHLLAVASPGPDFAIVVRESVAHGRRAGIFSAI
                     GVGCGIFIHVAYSLLGIGLIVSQSIVLFNALKWLAAAYLLYIGIRALRAKPASPSDAE
                     LSLREGERSPRAAFVAGFVTNGLNPKATLFFLSLFTVVINPHTPLPVQAGYGVYLAVA
                     TALWFCMVAQLFSHRRVRAGFARMGHWFDRLMGAVLVGLGVKLAFSELR"
     gene            9273..10238
                     /locus_tag="VFF22_17420"
     CDS             9273..10238
                     /locus_tag="VFF22_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017845949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HZX18634.1"
                     /translation="MSNSHRAVFLDHSSLDLGDLNLSSLSACFADLQLYPHSTQAQTI
                     ERLQGAQVAISNKVPLNAEVFAACPELKLVLVAATGTNPIDLAAARVHGVSVSNCQGY
                     GTPSVAQHTLMLLLALATRLPDYQQDVRAGRWQQAQQFCLLDYPIVELHGKTLGLLGH
                     GELGGAVAKLAEAFGMRVLLGQLPGRPARADRLPLDELLPQVDALTLHCPLNSDTLNM
                     IDARELSLMKPTAFLINTARGGLINEQALADALRAGHLGGAATDVLTQEPPKDGNPLL
                     AADIPRLIITPHSAWGSREARQRIVGQLSENALAFFNGAAIRVVS"
     gene            10267..11265
                     /locus_tag="VFF22_17425"
     CDS             10267..11265
                     /locus_tag="VFF22_17425"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX18635.1"
                     /translation="MDPRSEVLLRQADLFNGPLLLAGLPADDLLGHLHEAHGWSWHAG
                     EHALLDARFSNRCHFGTQPGARDFQAAVLFLPKSRELTEYLLNALAARLGGRELFLVG
                     EKRAGIERAAKQLASFGQARKLDSARHCQLWQVRVEHAPATPDLHSLAQRYEQALADG
                     PLQVLSLPGVFSHGRLDVGSALLLEHLDGLPSGHILDFGCGAGVLGAALKRRYPDSQI
                     ILLDVDAFAIESSRLTLAANGLQADVINGDGIDAAPTGLSAILSNPPFHQGVHTHYQA
                     TEHLLRQASRHLASKGELRLVANSFLKYPPLIEQHLGPCQTLADAKGFRIYRASKS"
     regulatory      11299..11501
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            11510..12094
                     /locus_tag="VFF22_17430"
     CDS             11510..12094
                     /locus_tag="VFF22_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HZX18636.1"
                     /translation="MLESLFVPTFIVALAEIGDKTQLLALLLAARFRKPWPIIWGIVV
                     ATLANHFAAGAIGNWVASFFSPATLSWILAASFVAVALWTLIPDKLDDDESSKLKRYG
                     PFLTTLVAFFLAEMGDKTQVATVMLAAQYPHFILVVIGTTLGMLLANVPVVLAGNFAA
                     DRLPLTLIRRLAACAFAALAVYAGYQALKFSGLI"
     gene            12205..12648
                     /locus_tag="VFF22_17435"
     CDS             12205..12648
                     /locus_tag="VFF22_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketosteroid isomerase-related protein"
                     /protein_id="HZX18637.1"
                     /translation="MSLDDRKKLVRHHIDLSWNKGRTALAEQLHSKDFLYKSSFIGRP
                     LTSSAFAQMVQDIRHAMPDLQVVIEECIAEDNKVVTWSTLIGTIQKPALGYPPSDKVL
                     SISAMAFWTLTPSNEIQEICTMFDMESFRSQLGLATQPFAAKALP"
     gene            complement(12706..13518)
                     /locus_tag="VFF22_17440"
     CDS             complement(12706..13518)
                     /locus_tag="VFF22_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZX18638.1"
                     /translation="MNTPFRLAGLALCSLLTACQAVNTTSGGAVGVERKQYMFSMLST
                     QEVNQMYAQSYQQTLGEASSKGVLDKTSNNAKRLRTIADRLIKHAPLFRPDAAQWQWE
                     VNLIKSPELNANCGPGGKIIFYSGIIEKLKLTDDEIAAIMGHEMAHALREHSREAMSK
                     AYGVEMAKQGAGALLGLGSDSLALADAAVQYGMMLPNSRGNENEADLIGLELAARGGY
                     NPNAAVSLWQKMAAASEGAPPEFMSTHPASSSRIASLQAAIPKVMPLYEQAR"
BASE COUNT         2896 a         4429 c         3809 g         2604 t
ORIGIN      
        1 cagacacgcc aacgcgggag caagctcccg cgcggcggat catgccgaac gcgactcgcg
       61 tcacgcccgg ccagagcggc ataagcttgc gcctatttat ttgttgtctt tgacctcttc
      121 gaactcagcg tcgaccacgt cgtcgcctgc gtccttggct tgctcagcac ccggggcttg
      181 cgcgcctggc tgcggctgct cggcgtacat cttctgcgca agcggtgcgg tcacttcgga
      241 cagcgcggtc atcttggcgt cgatttcagc tttgtcgtcg cccttgatcg ctacttccag
      301 cgcgctcaat gcggtttcga tagcagtctt ctcgtcagca gtggccttgt cgccggcctc
      361 ggtgatcatc ttgcgagtgg cgtgcaccag ctgatcgcct tggttacgcg cggtcgccag
      421 ctcttcgaac ttgcgatctt cctcggcgtt gacttcggca tcacgcacca tctgctgaat
      481 ttcttcttca gacagaccgg agttggcttt gatcacgatc gactgagtct tgccggtcgc
      541 cttgtctttc gcgccgacgt gcaggatgcc gttggcatcg atatcgaagg tgacttcgat
      601 ctgcggtacg ccacgcggag ccggcggaat ctcggccagg tcgaatttgc ccagggactt
      661 gttaccgctg gcttgcttgc gctcgccctg cagtacgtga atggtcacgg cgccctggtt
      721 gtcgtcggct gtcgagaaca cttgcgattt cttggtcgga atcgtggtgt ttttctcgat
      781 cagcgcagtc atcacgccgc ccatggtttc gatacccagg gtcagcgggc taacgtcgag
      841 cagcagcacg tctttcacat cgcccgacag taccgcgccc tggatagcag cacccatggc
      901 cacagcttcg tccgggttga cgtctttacg cgcttcttta ccgaagaaat cagtaaccag
      961 cttctgcacc agcggcatac gggtctgacc gccaaccagg atcacgtcgt tgatcgcacc
     1021 gatgtcgata ccggcatctt tcagcgcgat acggcaaggc tcgatggtgc gctgcaccag
     1081 gtcttcaacc agcgattcca gcttggcgcg ggagatcttc acgttcaggt gcttaggacc
     1141 ggtggcgtct gcagtgatgt acggcaggtt cacatcggtc gactggctcg aggacagctc
     1201 gatcttggct ttttcagcgg cttctttcag gcgctgcatg gccagcgggt cacccttgag
     1261 gttcatgccg ctttctttct tgaattcgtc aacgaggtaa tcgatcagac gaatgtcgaa
     1321 atcttcacca ccgaggaagg tgtcaccgtt ggtggccaac acttcgaact ggtgctcacc
     1381 gtcgacttca gcgatttcga ttacagaaac gtcgaaggta ccaccaccca ggtcataaac
     1441 gatcacggtg tgatcgccct tggccttgtc cataccataa gccagagcgg ctgcggtcgg
     1501 ctcgttgatg atgcgcttga cgtccagacc agcaatacga ccggcatctt tggtggcctg
     1561 acgctggctg tcgttgaagt aggccggcac ggtgataacc gcttcggtca ctggctcgcc
     1621 gaggtagtct tcggcggtct tcttcatctt cttcagaatt tcagccgaga tttgtggcgg
     1681 cgccattttc tggccgttca cttcaaccca ggcgtcgttg ttgtcagctt tgacgatctt
     1741 gtacgggacc atctggatgt ctttctgcac gacatcttcg tcaaaacgac gaccgatcag
     1801 acgctttacc gcgtacaggg tgttgtgcgg gttggtcact gcctggcgtt tggccgactg
     1861 acccaccaga atttcgccat cgttggcata agccacgatc gacggcgtgg tgcgcgcgcc
     1921 ttcggcgttt tcgataactt tagcgacacc gttttccaga accgagacgc acgagttggt
     1981 ggtccccagg tcgataccaa taattttgcc catcttcact ctcccgaaac tttgataatc
     2041 ccgcagctga tcgttgtcag cggcggtagc actaaaacgc ttgactgttt aatgggggca
     2101 gctcggcgaa tttcaagcct gctcatcgat cgatggcggc gcttccaccg gtgccttgct
     2161 gaccaccacc atggcggggc gcagcagacg accattgagc atgtagccct tctggaacac
     2221 cttgagcacg ctgttaggct cgacgctggt gctttcctgc atcgccatcg cctgatggtg
     2281 ctcggcgttg aacggcgcgc catgcgggtc aatcgcttcc aactggtgac gcttgagggt
     2341 gtcgcctaac agcttgagtg tcagctccat accctcacgt actgccttga tcgcctcatc
     2401 atccggattc gacaactcaa gaccgcgctc caggctatcc accaccggca acaggtcgtt
     2461 ggcaaatttc tccagagcga atttgtgcgc cttttctaca tcctgctcgg cgcggcggcg
     2521 cacgttctgc agatcggcag ccatgcgcag cgactgatcc tgcgcagcag ccagttgctc
     2581 ttccagcgct tgcacgcgag caaccaggtc atcgcccgca gccgcttcgg cagctgtttg
     2641 tacatcgggg ttctgcgtat ccagggtctg ttcgtcagcc atgcgtctct cctcttataa
     2701 atccgacgcg agcctcaact cacgtacctg accgctatat ggggtcaaaa aatgtggttt
     2761 caaggacaac agacggattg tcagtccaca aaaaaacact gtataaataa ccagacatga
     2821 acttgggagg ccctcgccat gctggtgcac ctgtccgtac acaactacgc catcgttgaa
     2881 caccttgatc tagagctgga cgccggcatg agcgtaatca gcggggaaac cggtgccggc
     2941 aagtcgatca tgctcgatgc cctcggcctg accctgggtg atcgagccga cagcggcgtg
     3001 gtgcgtcccg gcgcagacaa ggcggacatc ctcgccagtt ttgacctgca cgacatcccc
     3061 gaagcccgcg cctggctcgc cgagcgtgat ctggacaatg acggcccgtg catcctgcgc
     3121 cgagtgatca ccgccgaagg ccgctcgcgt ggctatatca atggctcgcc ttgccctcaa
     3181 ggcgatctca aagccctcgg cgagctgttg atcgacatcc acagccagca tgagcaccaa
     3241 tctctgctca agcccgatac ccaccgccgc ctgctcgacg aatacgccgg cagccaggaa
     3301 ctggcgcgcc aggtgcagct cgccgcgcag cgctggaaac agaccaagaa tgagctggag
     3361 cgagtttcca gcatcggcga cgaacaacgc gcgcgtcatc aattgctcag ctatcaactt
     3421 gaagagctgg acaacctggc cttgggcgaa gacgagctgg acaacctgga acaggaacac
     3481 aaaaacctga ccaacgccga aagcctgctc agcgcctgcc gcctggtgat cgagcagtgc
     3541 agcgagaatg acgctggcaa cgtgctgtcc gccctgacca gcagccttaa tcgcctcagt
     3601 gccttccagg gccagcccgg cgcactgagc gaagccacca acctgctggc cagcgcgcaa
     3661 atccaggtag aagaagccgt cggcgagctg aaccgctttc tcgaccactt cgacgccgac
     3721 cccgaacgcc tgcgccagat ggaagagcgc ctggatgcca tctacaccct ggcgcgcaag
     3781 caccgcatcc agccaacgga gctgggcgcc atgcagcaac agctgttcga agagctggaa
     3841 agcctcaatg ccgacgacca ggccgccgaa cgcctgagcg acgaactggc cgccttcgag
     3901 cggcattacc aggaaaaagc ggcggaactc agcaccctgc gcaacaacgc cgccagtcag
     3961 ctggccagcg cggtcgagca ggaaatgcag accctcggca tgcccggcgg gcgtttcaac
     4021 atcaagctga gcgccaacag caacagcgag ccacacccca acggcttgga gcagctggag
     4081 tttctggtca gcgccaaccc tggccagccg ttgaaggctc tggcaaaggt cgcctcgggc
     4141 ggcgagctgt cgcgcatcag cctggcgatt caggtgatca ccgcacagac ctcgcgggtg
     4201 cccaccctgg tattcgatga ggtcgacgtc ggcatcggag gccccaccgc cgaagtggtc
     4261 ggccagctac tgcgccgcct cggtgaacgc ggccaggtac tgaccgtcac ccacctgccg
     4321 caagttgccg cgcaaggtca tcaacacctg ttcgtgcaca aagtgcgtga aagcgatgcc
     4381 acccgcaccg cagtgagcaa actcagccca gcgcagcggg tcgaggaaat cgcgcgtatg
     4441 ctcggcggcg ttgatctcac cgaggaatcc ctggcccatg cccggcagat ggtcggcagc
     4501 gcccaggcct gattgcacag cccccccaga aagcacaaag gcgaccctag ggtcgccttt
     4561 ttcatgcagc agccaaatca gcttttcttg cgcacataca gcacaagatt gtgatcaacc
     4621 agctcgaaac cgtgctcttt gacgatttcc ttctgacgct tttcgatctc ggcgtcgaaa
     4681 aactcgatca cgtcaccaga gtcgacacag accatatggt cgtgatggcc actgtccgcc
     4741 aattcgaaga ctgcatgacc gccatcgaag ttatgacgaa ccaccagacc tgctgcttca
     4801 aactgggtca gtacgcggta cacagtcgcc aaacctacgt cgtcacccgc ctccatcagc
     4861 gccttgtaga catcttcagc actcatgtga cgctgaccgg cgtggtcaag catttgcagt
     4921 atcttgaccc gtggcagggt cactttcagg ccagccttgc gtagttcgtt attttcaacc
     4981 atggtctgct ttctcacgga agcggcttcg cagcttcctt tattgcgggt atgatccggg
     5041 gttatattgc ccagccaaga tagtggaagt cgcccaccga tgcaaaaaac caaaatcctg
     5101 ctgaccagcc tcacccttac ggggctactc gcactcgccg gttgttcatt ccccggggtt
     5161 tataaaatcg acatccaaca gggcaatgtc gtcacgcagg acatgataga ccagttgcgc
     5221 ccgggaatga cccgcaagca agtgcggttt attatgggca accccctgct cactgacacc
     5281 ttccacgcca atcgttggga ctacctgtac agcatccagc cgggcggcgg taagcgcctg
     5341 caggagcgcg tcagcgtgaa tttcaacgcc gaagaccaac tggttggtct gtctggcgac
     5401 tttatgccag gcgtcagccg tgaccaggcc atcctgggtg aggacggcgg cgttaccact
     5461 ccgcaaacca ctcagcagcc gcaggttgaa gagaaacccg ctccaggttc gctgcttgaa
     5521 cagattcagc gcgaagtgga cgacgtgaaa gtcgtaccag tgccgactcc agagcctctg
     5581 gaaagcaccc cgcagtaaca tttgctgata aaaaagcccg gactcgtccg ggctttttcg
     5641 tttttggctg acctgctcag tacttaattc ccgccatcct cggccttggc ttgcgccgcc
     5701 ttggcagcac gctgcttgcg cacctctttc gggtcggcaa tcagcggccg gtagatctcc
     5761 acccgctccc cctcttccag cacgcgctca tcgggtttgc tcaccgcctt gccgaagatg
     5821 cccagcgggc tgttgtgcag atccagatcg gggaaatgcg cctgcatgcc ggactgcacc
     5881 gccgcctgac gcaccgtggt gccatagggc aggctcaggc gcagcagctt ctgtttgtcg
     5941 gccagggcgt agaccacttc gaccacgatg tttggcttat ccatagagtt gcttggccct
     6001 ttggcagaat gcatcgacca gggtattggt ggcctgattg aacagcggcc cgagggtcgc
     6061 ccgcaccaat ggcccggcgt aatcgaaggt cagatcaaga ctgatcttgc aggccttgtc
     6121 gcccaatgcc ttgaactccc aatgcccata cagctgggtg aacggcccct cgaccagagt
     6181 cagcttgatc agctctcccg gcaccagggt gttggccgtg acaaagcgct ggctcaaacc
     6241 acccttggcc accgccaggc tggcacgcat atgcgtgtcg cttgactcaa gcacttcgct
     6301 ggccgagcac cagggcagaa actgcgggta actggccaca tcattgacca gatcaaacag
     6361 cgcccgcgcg ggataaggca acagcgccga acgctgaata tgagtactca taacccaacc
     6421 acttggctaa ataggatgca ggcgatcatt tgctcagctc tgcgacaaac acgatcagca
     6481 cgccaattgg cgcaaccaca cgaatcaacc aatacaccac gttgaacagc agcgggcttt
     6541 tcatcgccag ctcttcgcgc accgcttcac gcttaagcac ccagccggca aatatggcga
     6601 acgccaggcc gccgaacggc aacagaatac ggctagtcag gtaatcgatg gtgtcgaaga
     6661 aattccgccc accttctgcg ccccactgga acaggtggaa accatcctcg gcgaagacaa
     6721 agaacttcgc ttcagccccc aagttaaagg acaacaccgt gcccatgcca accacccaac
     6781 agaggaacgc caacacgctg gtcaccagca ctcggctgcg cttggtacgc tcgaccaaat
     6841 aggccaccgc aggctccagc atggagatcg acgaggtcca ggcagcgatc agcaccagga
     6901 taaagaacag cgtgcccatc acctggccaa aggcgatatt gccgaacgcc accggcaggg
     6961 tgacaaacat cagccccggc cctgcgccag gctccagacc accggcgaag acgatgggaa
     7021 acagcgccat cccggcggtg agggccacca gagtatcgag gatgccgacg gtgagaatgg
     7081 tgccgccaat cgaggcgttc ttcggcatgt acgagccgta gaccatgatc gcgcccacgc
     7141 cgacgctcag ggtaaagaag gcatgcccca tggcggccag cacgccatca agcacgcggt
     7201 ccggctggaa atcgaataga aagtggaagc cctgcataaa gtgcccggta gtcatgctgt
     7261 aacccagcag caccagcagc aaaacgaaca acagcggcat catgattcgc aggctgcgct
     7321 caagcccggc caccacgcca cgggcgataa caaagccggt cagcagcata aacagcgtat
     7381 gccacagggt cagccgccat ggatccgccg tcagcgcacc aaacgccgcg ccagcgccct
     7441 cggcactcac gccggcaaat tgcccgccgc ccatgctgag gatgtaatcc agcgaccagc
     7501 ctgccaccac gctgtaaaac gacaaaatca gcagagcagc aaccatcccc accagcgccg
     7561 cccaggacca tttcggagaa gcgcccgact cgatagccag ggtcttgagg gtattcaccg
     7621 ggctttgccg gccgcgccga ccgatcaggg tttcggcgag catgatcggc aagccgacca
     7681 taaagatgca caccaggtac atcatcacga aagccccgcc gccgtacatg ccggtcatat
     7741 aggggaactt ccagatattg cccaagccaa ctgccgaacc ggtcgctgcc agaataaaca
     7801 cccaacggct ggcccaggca ccgtgtatcg aaaccttatc gttcgccatg ctcggctagc
     7861 ctgctctact gaaaaaagat cgcgcattgt cggggattcg ccagggcggc tcaagcgcag
     7921 ccgacgccac gcagcaccag gcgtctggca aagctgattt tgccctactg cacacatagc
     7981 gcccgcgcca gtgactccct ataatgcgcg ccctatggct aaacaaaaga aacatccgca
     8041 aggcaccatc gcgcaaaaca aaaaggcgat gcacgactac ttcgtcgaat ccaggttcga
     8101 ggcaggcttg gttctggccg gctgggaagt gaaaagtctg cgcgccggca aggctcagtt
     8161 ggtcgacagc tacgtgctgc tgaagaacga cgaagcttgg ctgatgggct gccacatcac
     8221 cccactgacg gcggccagca cccatgtgat cgctgacccg acgcgcaccc gcaagctgct
     8281 gctgaataaa cgcgagctgg acaagctgtt cggcgccgta cagcagaaag gctatgcctg
     8341 cgtggccatg tcgatttact ggaaggcgca tctgatcaag tgcgagatcg ccctggccaa
     8401 gggcaagaag gacttcgaca agcgccacac agagaaagaa cgcgacgccg accgcgaagt
     8461 gcagcgcgct atgcgcagca aaggcaaaga cgagtaaatc gactctgcac taatgaaaac
     8521 gccccgcatt gcggggcgtt tttttgttac tgcgttcgac tcagcgcaac tcgctaaacg
     8581 ccagcttgac gcccaaaccc accagcaccg cccccatcag ccgatcaaac cagtgcccca
     8641 tccgggcaaa accggcacgc acccgccgat ggctgaacag ctgcgcgacc atgcagaacc
     8701 acagcgccgt ggccaccgcc agatatacgc catagcccgc ctgcaccggc aatggcgtat
     8761 gcgggttgat caccacggta aacagcgaca ggaagaacag cgtggccttg gggttcaggc
     8821 cattggtcac aaagccggcg acaaaggccg cacgcggcga acgctcgccc tcacgcaggc
     8881 tcaactcggc gtcactcggg cttgccggtt tggcccgcaa tgcccgaatg ccgatataca
     8941 gcaggtaagc cgcggccagc cacttgaggg cgttgaacag cacaatcgac tgcgacacga
     9001 tcaaaccgat acccagcagc gaataggcca catggataaa aatcccgcag cccacgccaa
     9061 tcgcactgaa gatgccggca cgccgcccgt gcgccacact ctcacgcacc acaatcgcga
     9121 aatccgggcc agggctggcc acagccagca agtgaatcag ggccacagct aaaaattcgg
     9181 tccagtacat gaggcggctc cgcgacgtcg aaatgattct gttaggctcg ccaccttact
     9241 ccccgcccaa acccgcagga tagatacagc tgatgagtaa cagtcaccgc gccgtctttc
     9301 tcgaccacag ctcactcgac ctgggcgacc tcaacctgtc cagcctgagc gcctgctttg
     9361 ccgatctgca gctgtatcca cacagcaccc aggcgcaaac catcgaacgc ctgcagggcg
     9421 cacaggtagc gatcagcaac aaggtgccgc tgaatgccga ggtgttcgcc gcctgcccgg
     9481 agctgaagct ggtgctggtg gccgccaccg gcaccaaccc gattgacctg gctgccgccc
     9541 gcgtccatgg cgtcagcgtg agcaactgcc agggctacgg cacgccatcg gtggcgcaac
     9601 acacgctgat gctgctattg gccctggcca cgcgcctgcc cgattaccag caggacgttc
     9661 gcgccggccg ctggcaacag gcacagcagt tctgcctgct cgattacccg atcgtcgaac
     9721 tgcacggcaa gaccctcggc ctgctcggcc atggcgaact gggcggcgcg gtggccaaac
     9781 tggccgaagc ttttggtatg cgcgtgctgc tcggccagct gccaggccgt cctgcgcgtg
     9841 ccgaccgcct gccactggat gagctgctac cccaggtcga tgcacttacc ctgcattgcc
     9901 cgctgaacag cgacacccta aacatgattg atgcccgcga actcagcctg atgaagccta
     9961 cagcctttct gatcaatact gcccgtggcg gcctgatcaa tgagcaggct ctggctgacg
    10021 cactgcgcgc cggccacctg ggcggcgcgg caaccgacgt gctgactcag gaaccaccga
    10081 aagacggcaa ccccttgctc gctgccgata ttccacgact gatcatcact ccgcatagcg
    10141 cctggggcag tcgcgaagcg cggcaacgca tcgtcggcca gctcagtgaa aacgcgctgg
    10201 ccttcttcaa cggcgcagcc atccgcgtag tcagctaagc tacgcgactt ttttcgagga
    10261 gcgcttatgg acccgcgaag cgaagtactg ctgcgccagg ccgatctatt caacggcccg
    10321 ctgctgcttg ccggcttgcc ggcggatgac ctgcttggcc acctccatga agcccacggc
    10381 tggagctggc atgccggcga gcacgcgctg ctcgatgccc gcttcagtaa tcgctgccac
    10441 tttggtacgc aacctggcgc acgcgacttc caggcagccg tgctgtttct gccgaaatcc
    10501 cgagagctga ccgaatacct gctcaatgcc ctggcggcgc gcctcggcgg gcgcgagctg
    10561 tttctggtcg gagagaaacg cgccggcatt gaacgcgccg ccaagcagct ggcgagcttt
    10621 ggtcaggcac gcaagctcga cagcgcgcgg cactgccagc tctggcaggt acgggttgag
    10681 cacgcacccg caacgccgga tctgcacagc ctggcgcaac gctacgaaca ggccctggcc
    10741 gacggcccac tgcaggtact cagcctaccc ggcgtattca gccatggacg cctggacgtt
    10801 ggcagcgcgc tgttacttga gcaccttgat ggcttgccca gcgggcatat tctggatttc
    10861 ggctgcggag ccggcgtact cggcgcggcg ctgaaacgcc gctacccgga cagccagatc
    10921 atcctgctgg atgtcgacgc ttttgccatc gaaagtagcc gcctgacatt ggccgccaac
    10981 ggcctacagg ctgacgtaat caatggtgac ggcattgatg ccgcgcccac gggcctgagt
    11041 gcgatcctta gcaacccgcc ctttcaccag ggcgtgcaca cgcactacca ggccacagaa
    11101 cacctgctgc gtcaggccag tcgacacctg gccagcaaag gtgaattgcg cctggtcgcc
    11161 aacagctttc tcaaataccc gccgctgatc gaacaacacc tcggcccctg ccagaccctg
    11221 gccgacgcca agggttttcg catctaccga gccagcaaaa gctaaacgag gcttgcccta
    11281 tcagggatgg gcgggcagaa tgcgcgcgtc ctaggggagt agtctcccgc gagcgccagc
    11341 tcgcccggca cgcgtcaaca tacttgctca gcagagcatg gcgcgtgcga cccaaggccc
    11401 actcagaggg gcctgcggtt tgacaagacc tatgacacgc acaccttacc cggggcggga
    11461 aggctgtacg tgtcatagcc gtgtcgaccc gcccctttag gaaagcctga tgctcgaatc
    11521 cctgtttgtt ccaaccttta tcgttgcctt agccgagatc ggcgacaaga cgcagctgct
    11581 cgctctgctg ttggccgcac gctttcgcaa accctggccg atcatctggg gcattgtggt
    11641 cgccactctg gccaaccatt tcgccgcagg cgctatcggc aactgggttg ccagcttctt
    11701 ctctcccgcc accttgagct ggattcttgc cgccagcttt gtagccgtag cgctgtggac
    11761 gctgatcccc gataagctgg atgacgacga aagctccaag ctcaagcgct acggtccgtt
    11821 cctgaccacc ctggtagcct tcttccttgc ggaaatgggc gacaagaccc aggtcgccac
    11881 ggtgatgctg gccgcgcaat acccgcactt tattctggtg gtgatcggca ccaccctggg
    11941 catgctgctg gccaacgtgc cggtggtact ggccggtaac tttgctgccg accgcctacc
    12001 gctgacactg attcgccgcc tggccgcctg cgcctttgct gcactggcgg tgtatgcggg
    12061 gtatcaggca ctgaaattca gcgggctgat ttgacatcaa tttggcgcca gagtgctggc
    12121 gtttcggcca atacttcggc ttcctgacga ctgctacagt gcgcaacatt ctgcccacct
    12181 cgctcagcag tcagggagac aagcatgtcg ctggatgatc ggaaaaagct cgtacgtcac
    12241 catatcgacc tgtcgtggaa caaaggccgc accgcgctgg cagaacaact gcacagcaaa
    12301 gactttcttt acaaaagctc gtttatcggc cggccactga ccagctcggc ctttgcccag
    12361 atggtgcagg acatccgcca tgccatgcct gacctgcagg tggtgattga ggaatgcatt
    12421 gccgaagaca acaaagtggt cacctggagc accctgatcg gcaccatcca gaaacccgcg
    12481 ctcggctacc cacctagtga caaggtgctg agcatctcgg ccatggcctt ctggaccctt
    12541 accccaagca atgagattca ggaaatctgc accatgttcg acatggaaag cttccgctcg
    12601 caactgggcc tggccaccca gccctttgct gccaaggcac tgccctgaat atataacgtc
    12661 caacaaaaag ccctcacctg agggcttttt gttgtttaaa gcgctttagc gcgcctgctc
    12721 atacaatggc atcaccttgg gaatcgccgc ctgcagagaa gcgattcggc tgctcgatgc
    12781 cgggtgcgtg ctcataaatt caggcggcgc gccttcgctg gctgcggcca ttttctgcca
    12841 caagctgacc gcggcattgg ggttgtagcc accgcgggct gccagttcca ggccgatcaa
    12901 atcggcctcg ttttcattgc cacggctgtt cggcagcatc atgccgtact gcaccgcagc
    12961 atcggccagc gccaggctat cggagcccag gccgagcaag gcacccgcgc cctgtttggc
    13021 catctcgaca ccataggcct tggacatcgc ctcacggctg tgctcgcgca gggcgtgagc
    13081 catttcgtgg cccatgatcg cggcgatctc atcgtcggtg agcttgagct tctcgatgat
    13141 cccgctatag aaaatgatct tgccacctgg gccgcagttt gcgttcagct cagggctctt
    13201 gatcagattg acctcccact gccactgcgc cgcatcagga cggaacagcg gggcatgctt
    13261 gatcaggcga tcggcaatgg tgcgcaaacg cttggcgttg ttgctggttt tatccagcac
    13321 acccttgctg gaggcctcgc ccagagtctg ctggtaggac tgcgcataca tctgattgac
    13381 ttcttgggtg gagagcatgc tgaacatgta ttgcttacgc tcaacaccta cagcgccgcc
    13441 actggtggtg ttcacggcct ggcaggctgt cagcagactg cagagcgcca gaccggcaag
    13501 acgaaatggg gtattcatgt cagttctcct tcacgacagg gaccgtatgc taggccgtgc
    13561 accaaggcaa aacaacccgc acgacttgca ctatggccct caaggctctt tgctagatac
    13621 agcggcgcgc taatgctttg aagctacgcg gccgataagc cagggacagg cgtaacccac
    13681 acgctgagga gtccctatgc gattcaagtc gatccagtcc tccgttgcac ttatggcg
//
LOCUS       DASRUM010000257         6951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1791276, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000257 DASRUM010000000
VERSION     DASRUM010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791276"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..636)
                     /locus_tag="VFF22_17445"
     CDS             complement(16..636)
                     /locus_tag="VFF22_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZX18639.1"
                     /translation="MGNHKIEIRRRNVEKILLAAEKVFAEKGHGGTAMGDIAEQAELP
                     RSNLHYYFSTKDELYRAVLLDLLEVWKQDALCFEMYDDPRVVLSTYIRAKMNHSRSRP
                     HGSKVWANEIIHGAPMLGDALDDSLYSWAKMKEAKIRQWVEDKRILAVEPSALLYMIW
                     ASTQHYADFSHQVAILNDHQALSELQFERAVQTVTSVILRGIGLEP"
     regulatory      778..871
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            894..2288
                     /gene="ahcY"
                     /locus_tag="VFF22_17450"
     CDS             894..2288
                     /gene="ahcY"
                     /locus_tag="VFF22_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HZX18640.1"
                     /translation="MSAAFNDFKVADISLAAWGRRETIIAESEMPALMGLRRKYAGEQ
                     PLKGAKIIGCIHMTIQTAVLIETLTALGAEVRWSSCNIFSTQDQAAAAIAAAGIPVFA
                     WKGETEEEYEWCIEQTILKDGQPWDANMILDDGGDLTEILHKKYPQMLERIHGVTEET
                     TTGVHRLLDMLAKGELKIPAINVNDSVTKSKNDNKYGCRHSLSDAIKRGTDHLLSGKQ
                     ALVIGYGDVGKGSAQSLRQEGMIVKVTEVDPICAMQACMDGYEVVSPFIDGINDGTAA
                     SIDAQLLGKIDLIVTTTGNVNVCDANMLKALKKRAVVCNIGHFDNEIDTAFMRKNWAW
                     EEVKPQVHKIHRTGAGSFDPHNDDYLILLAEGRLVNLGNATGHPSRIMDGSFANQVLA
                     QIFLFGQKYADLSPAQKAERLTVEVLPKKLDEEVALEMVKGFGGVVTQLTKTQADYIG
                     VTVEGPFKPHAYRY"
     gene            2375..3220
                     /gene="metF"
                     /locus_tag="VFF22_17455"
     CDS             2375..3220
                     /gene="metF"
                     /locus_tag="VFF22_17455"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HZX18641.1"
                     /translation="MSQERRYSFEFFPTKTDAGHEKLMNVARQLAGYNPDFFSCTYGA
                     GGSTRDRTLNTVLQLDGEIKVPAAPHLSCVGDSKAELRELLTLYKNAGIKRIVALRGD
                     LPSGMGMASGELRYANELVSFIREETGDHFHIEVAAYPEMHPQARNFEDDIANFVRKA
                     KAGADSAITQYFFNADCYFYFVERVRKLGVDIPVVPGIMPITNYSKLARFSDACGAEI
                     PRWVRKQLEAYGDDTDSIQAYGEQVITEMCERLLQGGAPGLHFYSMNQAEPSLAIWNN
                     LKLPR"
     gene            3464..4837
                     /gene="ppnN"
                     /locus_tag="VFF22_17460"
     CDS             3464..4837
                     /gene="ppnN"
                     /locus_tag="VFF22_17460"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HZX18642.1"
                     /translation="MAARKKTNASVTPKGSLETLSQREVQQLRETGSGSVYALFRQCA
                     LAILNTGSHSDNAKTILEAYPDFEIRILQQDRGIRLELFNAPADAFVDGEMIASTREM
                     LFSALRDIVYTQSELENKRVDFGSSQGITDYVFHLLRNAHTLRAGVEPKIVVCWGGHS
                     ISTEEYKYTKKVGHELGLRGLDICTGCGPGVMKGPMKGATISHAKQRNVHGRYLGLTE
                     PGIIAAEAPNPIVNELVILPDIEKRLEAFVRVGHGIIIFPGGAGTAEELLYFLGILMH
                     PDNQNIPFPLIMTGPRSAAAYLEQLHEFIGATLGEAAQQRYSIIINDPEQVAREMAEG
                     IKAVKQFRREHNDAFHFNWLLKIDESFQHPFEPTHANMASLKLTRQQPVHELAAHLRR
                     AFSGIVSGNVKDKGIRLIEQYGPYEITGDRDIMQQLDQLLQAFVDQHRMKLPGNSAYV
                     PCYKLIS"
     gene            5067..6941
                     /locus_tag="VFF22_17465"
     CDS             5067..6941
                     /locus_tag="VFF22_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZX18643.1"
                     /translation="MSFASLGLSEALVRAVESAGYTQPTPVQQRAIPAALQGRDLMVA
                     AQTGTGKTGGFALPILERLFPNGHPDREQRHGPKQPRVLVLTPTRELAAQVHDSFKLY
                     ARDLKFVSACIFGGVGMNPQVQALAKGVDVLVACPGRLLDLANQRAIDLSHVEILVLD
                     EADRMLDMGFIHDVKKVLAKLPAKRQNLLFSATFSKDITDLAGKLLHNPERIEVTPPN
                     TTVERIEQRVFRVQASHKRALLAHLITAGAWEQVLVFTRTKHGANRLAEYLDKHNLPA
                     VAIHGNKSQNARTKALADFKAGNVRILVATDIAARGLDIDQLPHVVNFELPNIEEDYV
                     HRIGRTGRAGRSGEAISLVAPDEEKLLKGIERMTKQKIGDGDLLGFDASTVEAERPEV
                     REPQKPRQPRGAKPEGERHGGGRKDKGKDSGKEHSRAAKPQGGRSQQPRNSSSGRAAA
                     PAVPALPPDRAPDEFRDDEIDNFGNRADYVSPNQNKQQNRGRRPGTPAAPSGPSASNG
                     VGLGARPAKPPRSNGGGANGAQGRRQGAGSGGQGGQGRNGSSRARPPRDTRTTSLNRD
                     ELPRREGAAKAPSDKQPVIVHKGSRLDRFPTPEQLDELASARPRGEKPALLTRNREES
                     "
BASE COUNT         1482 a         2308 c         2008 g         1153 t
ORIGIN      
        1 cccgcgatca ggctttcagg gctccagacc gatcccacgc aatatcacac tggtcacggt
       61 ctgcaccgcc cgctcgaact gcagttcgga cagcgcctgg tgatcgttga gaatcgccac
      121 ctgatggctg aagtcggcgt agtgctgagt cgaggcccag atcatataga gcaaggcgga
      181 cggttcgacg gcgaggatgc gtttgtcctc aacccactgg cggatcttcg cttccttcat
      241 cttggcccag ctgtagaggc tgtcgtcgag ggcgtcaccg agcattggcg caccatggat
      301 gatctcgttg gcccagactt tggagccgtg cgggcggctg cgcgagtggt tcatcttggc
      361 gcggatatag gtactcagta ccacgcgcgg atcgtcatac atctcgaagc acagcgcgtc
      421 ctgcttccac acctccagca ggtccagcag caccgcgcgg tacagctcgt ccttggtgct
      481 gaagtaatag tgcaggttgg aacgcggcag ctcggcctgt tcggcgatat cgcccatggc
      541 ggtgccgcca tggcctttct cggcgaacac cttttccgcg gccaggagaa ttttctcgac
      601 gttgcggcgg cgaatctcga ttttgtgatt gcccatgaca ggtccatgac tacgcaatgc
      661 agcaaggcta ccatccgcgc cgaggcgggg cgacggaaag cctttggcta aatccccagc
      721 atggctataa tcacccccgc ttcacgcaac cggcacagct ccggttgctc ccgccacccg
      781 tccgaggggc gctgcagcaa actctggcta ccggagtttt gtcaggctcg gatggggcgt
      841 taaccatacg catcaacggc gcccattcgc agacaacgaa tggagagctt tttatgagcg
      901 ccgcttttaa cgatttcaaa gtcgccgaca tttccctggc tgcctggggc cgtcgtgaaa
      961 ccatcatcgc cgaatccgag atgccagccc tgatgggcct gcgccgcaaa tacgccggcg
     1021 agcagccgct caagggcgcg aagatcatcg gctgcatcca catgaccatc cagaccgccg
     1081 tactgatcga aaccctgact gcattgggtg ccgaagtacg ttggtcgtcg tgcaacatct
     1141 tctcgactca ggaccaggcc gctgccgcta tcgctgctgc cggcattcca gtattcgcct
     1201 ggaagggcga gaccgaagaa gagtacgagt ggtgcatcga gcagaccatc ctcaaggatg
     1261 gccagccgtg ggatgccaac atgatcctcg acgacggcgg cgacctgacc gagatcctgc
     1321 acaagaagta cccgcagatg ctcgagcgca tccacggcgt caccgaagaa accacaaccg
     1381 gcgtacaccg cctgctggac atgctggcca agggcgagct gaaaatcccg gcgatcaacg
     1441 tcaacgactc ggtgaccaag agcaagaacg acaacaaata cggctgccgt cacagcctga
     1501 gcgacgcgat caagcgcggt accgaccacc tgctgtccgg caagcaagcg ctggttatcg
     1561 gctacggcga cgtgggcaag ggctcggcac aatcgctgcg tcaggaaggc atgatcgtca
     1621 aggtcaccga agttgacccg atctgcgcca tgcaggcctg catggacggc tacgaagtgg
     1681 tttcgccgtt tatcgacggc atcaacgacg gcaccgccgc cagcatcgac gcacagctgc
     1741 tgggcaaaat cgacctgatc gtcaccacca ccggtaacgt caatgtctgc gacgccaaca
     1801 tgctcaaggc cctgaaaaag cgcgcagtag tgtgcaacat cggtcacttc gacaacgaaa
     1861 tcgacaccgc tttcatgcgc aagaactggg cgtgggaaga agtgaagcca caggtgcaca
     1921 agatccaccg caccggcgct ggcagcttcg atccgcacaa cgatgactac ctgatcctgc
     1981 tggccgaagg ccgtctggtt aacctgggca acgccactgg ccacccaagc cggatcatgg
     2041 acggctcgtt cgccaaccag gtgctggcgc agatcttcct gttcggccag aaatacgccg
     2101 acctgtcgcc agcccagaaa gccgagcgcc tgaccgtgga agtgctgccg aagaagctcg
     2161 acgaagaagt ggccctggaa atggtcaaag gcttcggcgg cgtcgtcact cagctgacca
     2221 agacccaggc cgactacatc ggcgtgaccg tcgaaggtcc gttcaagccg cacgcttacc
     2281 gctattgatg cgtagggtgg gttggccgca tggccgaccc atcgagaacc ctggtgggtt
     2341 ggcctgcggc tgacccaccc tacggaatgt ccttatgtct caagaacgcc gttacagctt
     2401 cgagttcttc cccaccaaga ccgacgccgg gcacgaaaaa ctgatgaatg tggcgcgcca
     2461 actggcgggc tataacccgg atttcttctc ctgcacctac ggtgccggtg gctccacccg
     2521 cgaccgtacg cttaataccg tgctgcaact cgatggcgag atcaaggtcc cggctgcgcc
     2581 gcacctgtcc tgcgtcggcg acagcaaggc cgagctgcgc gaactgttga ccctgtacaa
     2641 gaatgccggg atcaagcgca tcgtcgctct gcgcggcgat ctgccgtccg gtatgggcat
     2701 ggccagcggc gaactgcgct acgccaacga gctggtcagc tttatccgcg aagaaacggg
     2761 cgaccatttc catatcgaag tcgctgccta cccggaaatg cacccgcagg cacgcaactt
     2821 cgaagacgac atcgccaact tcgtgcgcaa ggccaaggcc ggtgccgaca gtgcgatcac
     2881 tcagtacttc ttcaacgccg actgctactt ctacttcgtc gagcgcgtgc gcaagctggg
     2941 tgtggatatt ccggtggtgc cgggcatcat gccgatcacc aactacagca agctggcgcg
     3001 cttctccgac gcctgcggtg cggaaatccc gcgctgggtg cgcaagcaac tggaagctta
     3061 cggtgacgac accgacagca tccaggcgta tggcgagcag gtcatcactg aaatgtgcga
     3121 gcgcctgctg caaggcggcg ctccgggttt gcacttctac agcatgaacc aggccgaacc
     3181 gagcctggcg atctggaaca acctcaagct gccacgctaa cgcttcacgg cagccacctc
     3241 ggcacccgcg tcctgcgggt gccgatggtt ttcccggcca tgcgctatgg cccacctgac
     3301 cagacactgc agccttttca ccactcgccc ggcaaaccac ggtagccttt tgcaacgcac
     3361 taccgggtca gcccatagtc caaagtactc agtcacgacc atactttcag caagcgtcta
     3421 gccatgtagg gcttacgcgg tatcacaacc atggagcgcc gcaatggccg cacgaaaaaa
     3481 gaccaacgcc tcagtaaccc ccaaaggcag cctggaaacc ctctcgcaac gtgaagtcca
     3541 gcagctgcgc gaaaccggtt ccggcagcgt ctatgcgctg ttccgccagt gcgccctggc
     3601 gatcctcaac accggctcgc acagcgacaa cgccaagacc attctggaag cctacccaga
     3661 cttcgaaatc cgcatcctgc agcaggatcg cggcatccgt ctggagctgt tcaacgcccc
     3721 ggccgacgcc ttcgtcgatg gtgaaatgat cgccagcacc cgcgagatgc tgttcagcgc
     3781 cctgcgcgac atcgtctaca cgcagagcga gctggagaac aagcgggtcg acttcggcag
     3841 ctcccaaggc atcaccgatt acgttttcca cctgctacgc aacgcccaca ccctgcgcgc
     3901 cggcgtcgag ccgaagatcg tggtgtgctg gggcggccac tcgatcagca ccgaagaata
     3961 caaatacacc aagaaagtcg gccacgaact gggcctgcgc ggcctggata tctgcaccgg
     4021 ctgcggccct ggcgtgatga agggcccgat gaaaggcgcg accatttccc acgccaagca
     4081 gcgcaacgtg cacggccgct acctgggcct gaccgagccg gggatcatcg ccgccgaagc
     4141 gcccaacccg atcgtcaatg agctggttat cctgccggat atcgagaagc gcctggaggc
     4201 cttcgtccgt gtcggccacg gcatcatcat cttccccggc ggcgccggca cggccgaaga
     4261 gttgctgtat ttcctcggca tactgatgca cccggacaac cagaacatcc ccttcccgct
     4321 gatcatgacc ggaccacgca gcgccgctgc ctaccttgag cagctgcatg agtttatcgg
     4381 tgcgaccctg ggtgaagcag cgcaacagcg ctacagcatc atcatcaacg accccgagca
     4441 ggtcgcccgg gaaatggccg aagggatcaa ggcggtcaaa cagttccgcc gtgaacataa
     4501 cgacgccttc cacttcaact ggctgctgaa gatcgacgaa agcttccagc acccgttcga
     4561 gccgacccac gccaacatgg ccagcctcaa gctgacgcgc cagcaaccgg tacatgaact
     4621 ggcggctcac ctgcgccgag cgttttccgg gattgtctcg ggcaacgtca aggacaaggg
     4681 catccgcctg atcgagcaat acggccctta cgagatcacc ggcgaccgcg acatcatgca
     4741 gcagctcgat caattgctgc aggccttcgt cgaccagcac cggatgaaac tgcccggcaa
     4801 cagcgcctat gtgccctgct acaaactgat cagctgagcc ggtggaaccc gcgtcgttgc
     4861 tggcatggca acggcgcgca accaccgagc aggcagacgc tcgcacagac cggccgctct
     4921 gttatactcg ggccttcccg ccaggcttgc gcccggatgc tagcccccag aggtcagcag
     4981 taccggacgg gattgcgccc cccaagcgcg attcaagcca ggcaatacac cccatagggc
     5041 gacgccctca ctagacagga ttacacatgt cctttgcttc cctcggtctc tccgaggcat
     5101 tagtccgtgc ggtcgaatcc gccggctaca cccaacccac tccggtgcaa cagcgggcca
     5161 tccccgccgc gttgcaaggt cgcgacctga tggttgcagc ccagacgggt acaggtaaga
     5221 cgggcggttt tgccctgccg atcctcgagc gactgttccc caacggtcac ccggatcgcg
     5281 aacagcgtca cggcccgaaa caaccgcgcg tcctggtatt gaccccgacc cgcgaactgg
     5341 ccgcccaggt acatgacagc ttcaagctgt atgcccgcga tttgaaattc gtcagcgcct
     5401 gcatctttgg cggcgttggc atgaacccgc aggtccaggc cctggccaaa ggcgtcgacg
     5461 tactggtggc ctgccccggt cgcctgctcg acctggccaa ccagcgcgcc attgacctgt
     5521 cgcacgtgga aatcctcgtc ctcgacgaag ccgaccgcat gctcgacatg ggctttattc
     5581 atgacgtgaa gaaggtcctc gccaagcttc cggccaaacg gcagaacctg ctgttctcgg
     5641 cgaccttctc gaaagacatc actgatctgg ccggcaagct gctgcacaac ccggaacgca
     5701 tcgaagtcac gccgccgaac accacggtcg agcgcatcga acagcgtgta ttccgcgttc
     5761 aggccagcca caagcgcgcc ctgctcgctc acctgatcac cgccggcgcc tgggaacagg
     5821 tactggtgtt cacccgtacc aagcacggcg ccaaccgcct ggccgaatac ctcgacaagc
     5881 acaacctgcc ggctgtagcg atccacggta acaagagcca gaacgcccgc accaaagccc
     5941 tggctgactt caaggccggc aatgtgcgca tcctggtggc caccgatatc gccgcccgcg
     6001 gtctggatat cgaccagcta ccgcacgtgg tcaacttcga actgccgaat atcgaagaag
     6061 attacgtgca ccgcatcggc cgtactggcc gtgctggtcg tagcggtgaa gcaatttccc
     6121 tggttgctcc ggatgaagag aagctgctca agggcatcga gcgcatgacc aagcagaaaa
     6181 tcggcgacgg cgatctgctc ggttttgatg ccagcaccgt cgaggccgag cgcccggaag
     6241 tgcgtgagcc gcaaaagcca cgtcaaccgc gcggcgccaa gccggaagga gaacgccatg
     6301 gcggcgggcg caaggacaaa ggcaaggaca gcggcaaaga acacagccgc gcagccaagc
     6361 cgcagggcgg ccgcagccaa cagccgcgca actcgtcctc ggggcgcgct gccgcgccgg
     6421 ccgtgccggc actccctccg gaccgcgcgc cggatgagtt ccgcgacgat gaaatcgata
     6481 atttcggcaa ccgtgccgac tatgtaagcc cgaaccagaa caagcagcag aaccgtggcc
     6541 gtcggcccgg tacgcctgca gcgccaagcg gcccgagcgc cagcaatggc gtcggtctgg
     6601 gtgctcgtcc agccaagccg cctcgcagta atggtggcgg tgccaatgga gcacagggtc
     6661 gtcgccaagg cgcaggttct ggcggccagg gcggtcaggg ccggaatggc tccagccgtg
     6721 cacgtccgcc gcgcgacacc cgcaccacct cgctgaaccg tgacgagttg ccgcgccgtg
     6781 aaggtgccgc gaaggcaccc agcgacaagc agccggtgat cgtgcacaag ggctcgcgcc
     6841 tggaccgctt cccgacgccg gagcagctcg acgaattggc gagtgcacgt ccgcgcggcg
     6901 aaaagccggc cctactgacg cgcaaccgcg aagagtcata gtcagctagc c
//
LOCUS       DASRUM010000258        11511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1318500, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000258 DASRUM010000000
VERSION     DASRUM010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 11511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11511
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318500"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFF22_17470"
     CDS             <1..264
                     /locus_tag="VFF22_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HZX18644.1"
                     /translation="GPYLILPLLGPSNLRDTGGLVADFAAKSQINWLNVAEASTSHPE
                     ITLLEVVDQRSTVNFRYGQLNSPFEYEKIRFVYHEARKLQIAE"
     gene            339..782
                     /locus_tag="VFF22_17475"
     CDS             339..782
                     /locus_tag="VFF22_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding protein"
                     /protein_id="HZX18645.1"
                     /translation="MRVAQLAKAAAVSSETVRHYTDLGLLRPTRNPDNGYQEYSSADL
                     QRLRFIARARRLGFSLKDSQQILASADNGHAPCAEVRDLLSERLAALQAHIEECQRLA
                     LVMQNAFDDWADKGECAPDGQAICRLIEDFEPDIERRPATPCPAH"
     gene            complement(790..1116)
                     /locus_tag="VFF22_17480"
     CDS             complement(790..1116)
                     /locus_tag="VFF22_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010565019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18646.1"
                     /translation="MRSLLRGAVLISLLGLGACVQSPFATAPVTTPSGSSNHPRFAPP
                     PGVDSQWDAGLGVYVVGGASHLYYRERTYYRWSSGWSWASNPQGPWLSTDSSGVPPGL
                     YRRYAQ"
     gene            complement(1113..2315)
                     /gene="ccmI"
                     /locus_tag="VFF22_17485"
     CDS             complement(1113..2315)
                     /gene="ccmI"
                     /locus_tag="VFF22_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HZX18647.1"
                     /translation="MIEFWLCAGLLLLTALAFLLIPVLRARKVQAEEDRTALNVTLYQ
                     ERLLDLQGQHDAGALNAEQLANGRDEAARELLADTEGAQAQPTQRALGGKIPLIAALL
                     MPLLGYGLYQHWGAIDDVERAQTFAAQPKTIEEMTARLEAAVQNDPKSAESWYFLGRT
                     YMAQERAADAAVAFERAVGVAGREPELLGQWAQALYFSGNKQWTEQLQALTDEALKAD
                     PAEVTSLGLLGIAAYEAGQFQQAIERWQQLVAVLPAEDPSRMAIQGGIDRAREQLGAP
                     AADAAPAASQGLTVRVALAAELQAKVQPGDAVFIFARAVSGPPMPLAVKRLSVADLPA
                     EVSLSDSDAMMPQLKISGFEQVQLVARISRSGNATAGEWIGSSQPLASKTTELQQLVI
                     DSADSKAP"
     gene            complement(2315..2797)
                     /locus_tag="VFF22_17490"
     CDS             complement(2315..2797)
                     /locus_tag="VFF22_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HZX18648.1"
                     /translation="MKRLLSAAVLGLALLGAAQAAIDTYEFATEAERERYRNLVEELR
                     CPKCQNQNIADSDAPIAMDLRAQIFRMLEEGQSNEQIIDFLVSRYGDFVLYKPPVTAR
                     TLLLWYGPAGLLAGGFVLLGVILVRRRSKPGAAASGLSADEQQRLAALLNQTPVDKKD
                     "
     gene            complement(2794..3330)
                     /locus_tag="VFF22_17495"
     CDS             complement(2794..3330)
                     /locus_tag="VFF22_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017679188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HZX18649.1"
                     /translation="MKRLLLLLPLVLFLGVAVFLYRGLFLDPTELPSALIGKPFPAFS
                     LPAVQDGKTLSDADLKGKPALVNVWATWCVACRVEHPVLNKLAKLGVTIHGVNYKDDN
                     AAAQKWLVEFHDPYQLNINDARGTLGLDLGVYGAPETFLIDKDGIIRHKFVGVIDERV
                     WREQLAPLYQELVDEVQP"
     gene            complement(3327..5300)
                     /locus_tag="VFF22_17500"
     CDS             complement(3327..5300)
                     /locus_tag="VFF22_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HZX18650.1"
                     /translation="MIPELGHLAMILALCMALVQATLPLIGAWRGDRQWMSLAQPAAW
                     GQFSFMLFAFICLTYSFMVDDFTVAYVANNSNSALPWYYKFSAVWGAHEGSLLLWGLI
                     LAGWTFAVSIFSRQLPEEMLARVLAVMGIISIGFLLFLIITSNPFERLLFDTPANGRD
                     LNPLLQDFGLIIHPPMLYMGYVGFSVAFAFAIAALLGGKLDAAWARWSRPWTIVAWAF
                     LGIGIALGSWWAYYELGWGGWWFWDPVENASFMPWLVGTALIHSLAVTEKRGVFKSWT
                     VLLAIAAFSLSLLGTFLVRSGVLTSVHAFASDPERGVFILAFLLLVVGSSLALFAVRA
                     PVVKSQVGFALWSRETLLLVNNLILVVAAAMILLGTLYPLVLDAMTGAKLSVGPPYFN
                     ALFVPLMGLLMAMIAVGVLVRWKDTPLQWLLSMLAPVLIGSVVLAVIATFLVGDFNWA
                     VLGVCLLAAWVVLAGLRDIVEKTRHKGFFRGLPSLTRSYWGMQTAHLGMAVCALGVVL
                     TSMGSFERDMRMAPGESVEIGGYQFIFEGAEHFEGPNFTSDKGTVRILEDGKQIAVLH
                     PEKRLYTVQQSVMTEAGIDAGITRDLFVALGEPLENGAWAVRVHIKPFVRWIWFGALL
                     MGFGGFLAAADKRYRMKVRTRVRDALGMAGAQA"
     gene            complement(5297..5785)
                     /gene="ccmE"
                     /locus_tag="VFF22_17505"
     CDS             complement(5297..5785)
                     /gene="ccmE"
                     /locus_tag="VFF22_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003117796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HZX18651.1"
                     /translation="MNPVRKKRLYIVLAIIAGVGIAVALALSALQQNINLFYTPTQIA
                     NGEAPQDTRIRAGGLVEEGSVKRSSDSLDTDFVVTDGAKSVTIRFSGILPDLFREGQG
                     IVAMGKLDANGVLIADEVLAKHDENYMPPEVMQALEQSGMQQKHDAAKAAKQASPSEY
                     AQ"
     gene            complement(5782..5958)
                     /gene="ccmD"
                     /locus_tag="VFF22_17510"
     CDS             complement(5782..5958)
                     /gene="ccmD"
                     /locus_tag="VFF22_17510"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003296567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HZX18652.1"
                     /translation="MSFASFSDFIAMGTHGPYVWSCYAFSLAVLGLNVALPILARRRY
                     LQDEARRLRREESK"
     gene            complement(5955..6713)
                     /locus_tag="VFF22_17515"
     CDS             complement(5955..6713)
                     /locus_tag="VFF22_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HZX18653.1"
                     /translation="MMNWTWFHKLGSPKWFYEISSRWLPWLALAAAVLLVVGLVWGLA
                     FAPPDYQQGNSFRIIYIHVPAAFLAQSIYVMLAVAGIVGLVWKMKLADVALQQAAPIG
                     AWMTVIALVTGAIWGKPTWGTYWVWDARLTSMLILLFLYFGVIALGNAITNRDSAAKA
                     CAVLAIVGVVNIPIIKYSVEWWNTLHQPATFSVVSKPAMPFEMWMPLLIMVLGFYCFF
                     GAVLLMRMRLEVLTREARSSWAKAEVKALVEKGQ"
     gene            complement(6787..7458)
                     /gene="ccmB"
                     /locus_tag="VFF22_17520"
     CDS             complement(6787..7458)
                     /gene="ccmB"
                     /locus_tag="VFF22_17520"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HZX18654.1"
                     /translation="MSNVFTLLLAREARLLFRRPAELANPLVFFAIVIALFPLAVGPQ
                     TQLLQSLSPGLVWVAALLAVLLSLDGLFRSDFEDGSLEQWVLSPHPLALLVLAKVLAH
                     WLFSGLALVLLAPLLALMLGLPGRCLPVLLISLLLGTPVLSLLGAVGAALTVGLKRGG
                     LLLALLILPLYIPVLILGSGALQASLQGLPAVGHLLWLASLTALAVTLTPFAIAAGLT
                     ISVAE"
     gene            complement(7455..8090)
                     /gene="ccmA"
                     /locus_tag="VFF22_17525"
     CDS             complement(7455..8090)
                     /gene="ccmA"
                     /locus_tag="VFF22_17525"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HZX18655.1"
                     /translation="MTSPLLEAVALACERDWRMLFERLELRLVPGEMLQVAGPNGSGK
                     TSLLRLLAGLMQPTAGEIRLNGRPLAAQRAELARNLLWIGHAAGIKGLLTAEENLTWL
                     CALHQPAEREAIWQALAAVGLRGFEDVPCHTLSAGQQRRVALARLYLPGPPLWVLDEP
                     FTALDKAAVAQLESHLAAHCQQGGLVVLTTHHELTVKPAGYRELDLGQRTP"
     gene            8281..9798
                     /locus_tag="VFF22_17530"
     CDS             8281..9798
                     /locus_tag="VFF22_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HZX18656.1"
                     /translation="MAPTPPSTRPNAIAADLAVKLLQPMQGLLASGETAKAEVIAMKE
                     VAQSFQMLLKLTLNNGSQTTLEASSPRPLAQGTSLAVTALSETRLALSVLMGGDKSLT
                     SLDLDQLPVGTLLQGKVVAREQLLQGRSQQVIYKVLVSLLNTPLAGSKLSLETPLALP
                     LGSLLSAQVQGSQALNFLPLSGRLDQLALSQQLGNQSNRQGSLEGLFSALQGLRGNAE
                     MGESLRTSIDKLFGAMPDAAQLSSPKGLAMALENSGILLESKLLGGQGQALPTDLKAN
                     LLRLITQLLPNLSAGTPLAAANAGSALAQAMPALARDLLGSLGKATSRQQALNFPLPS
                     RLLQAMEGEADLETLLKLAAAAISRLQTHQLSSLAQSQVGPDGNLLTTWQLELPMRNQ
                     QELVPLQVKIQRDESNPQDKPEKKEVLWKVELAFDLDPLGPLQVQAQLAHGRFSSQLW
                     AERSSTVSLIDAELDNLRQRLNAAGVQVGELACSQGTPPRGPRTTLEQRWVDETA"
     gene            9795..10121
                     /locus_tag="VFF22_17535"
     CDS             9795..10121
                     /locus_tag="VFF22_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HZX18657.1"
                     /translation="MKKTAPRQAIALTYDGQNAPVLSAKGDDELAEAILAIAREYEVP
                     IYENAELVRLLARLELGDAIPEQLYRCIAEIIAFAWYLKGKAPAGSERDSDITPPLRL
                     LEGPAH"
     gene            complement(10145..10549)
                     /locus_tag="VFF22_17540"
     CDS             complement(10145..10549)
                     /locus_tag="VFF22_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HZX18658.1"
                     /translation="MLDALLIALVLVCLGLIGACVWLTRQLRDSIKLQGERDAVRDRR
                     LKELSRRLDAYLTGSVQMGEELHELRRVVAPLPDKLNQIEQRDPSSLSFTQAARLAGM
                     GASVDDLTQSCGLSKAEAELVSKMQQAKLQRS"
     gene            complement(10553..11032)
                     /locus_tag="VFF22_17545"
     CDS             complement(10553..11032)
                     /locus_tag="VFF22_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZX18659.1"
                     /translation="MKKSSAQGSDDPILQWVTFRLDNETYGINVMQVQEVLRYTEIAP
                     VPGAPSYVLGIINLRGNVVTVIDTRQRFGLDSTEVTDNTRIVIIEADKQVVGILVDSV
                     AEVVYLRQSEVETAPNVGNDESAKFIQGVCNKNGELLILVELDKMMSEEEWSDLESI"
     gene            complement(11138..>11511)
                     /locus_tag="VFF22_17550"
     CDS             complement(11138..>11511)
                     /locus_tag="VFF22_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZX18660.1"
                     /translation="GQELTPLFGQPDWFLGILPCQAGNLKVLDTARWVMPDRYRDDFR
                     EGLQYVISVEGYEWGLAVHQVSRSIRLDPSEVKWRSQRTQRPWLAGTVIEHMCALLDV
                     ASLAELIASGAAKRMAASQIH"
BASE COUNT         2490 a         3803 c         3333 g         1885 t
ORIGIN      
        1 gggccttacc tgattctgcc gctactgggg ccatcgaacc tgcgtgatac cggcggcctg
       61 gtggcggatt ttgcggcaaa aagccagatc aactggctca acgtcgccga agccagcacc
      121 agccacccgg aaatcacctt gctggaagta gtcgaccagc gctcaacggt gaacttccgc
      181 tacggccagc tcaactcgcc gttcgagtac gagaaaattc gcttcgtcta ccacgaggcg
      241 cgcaagctgc aaatcgccga ataaacctgc gagcttgacc caagggccgc ccccaggttt
      301 taacctggtg cggcctttgt cgattctgga gattgattat gcgcgtagcc caattggcca
      361 aagccgccgc agtcagcagt gagaccgtgc gtcactacac cgatctaggc ctgctcaggc
      421 ccacgcgcaa cccggataac ggctaccagg aatactccag cgcagacctg caacgcctgc
      481 gctttatcgc ccgcgcccgc cgtttgggct ttagtctgaa agacagccag cagatactgg
      541 ccagcgccga taacggccat gcgccctgcg cagaggtacg cgacctgctt agcgaacgtt
      601 tggcggcctt acaggcgcat atcgaggagt gtcagcgcct ggcgctggtg atgcagaacg
      661 ccttcgacga ctgggccgac aagggcgaat gcgcccccga tggtcaggcc atctgccgtt
      721 tgatcgaaga tttcgagccg gatatcgagc gccgcccagc gacgccctgc cccgcgcact
      781 aacccctagt tactgggcgt agcggcggta caagcccggc ggcacaccgc tgctgtcagt
      841 tgacaaccag ggaccctgcg ggttactggc ccagctccaa ccggagctcc agcggtaata
      901 ggtgcgctca cggtagtaca gatgagaggc gccacccacc acatacaccc ccaggcccgc
      961 atcccactgg ctgtccactc ccggtggcgg agcaaagcgt gggtggttgc tgctgccact
     1021 cggggttgtt accggcgccg tggcaaacgg gctctgcacg caagcaccga gtcccagcag
     1081 gctaatcagc acggcgccac gcagtaacga cctcatggtg ctttgctgtc agcgctgtcg
     1141 atcaccagct gctgcagctc ggtggtcttg ctggccagcg gctggctgct gccgatccac
     1201 tcaccggccg tggcgttgcc gctccgggag atacgcgcga ccagctgcac ctgctcgaag
     1261 ccggagattt tcagctgcgg catcatcgca tcgctgtccg acaggctgac ttcagccggc
     1321 aggtccgcca cgctcagacg tttgaccgcc agcggcatcg gcggcccaga gacggcgcgc
     1381 gcgaagataa acaccgcatc gcccggttgc accttggcct gcagttcggc agccagggca
     1441 acgcgcacag tcagaccttg gctagccgcc ggtgcagcat cggccgccgg cgcgccaagt
     1501 tgctcacggg cgcggtcaat cccgccctga atcgccatgc gcgaagggtc ttcggccggc
     1561 agaaccgcca ccagctgctg ccagcgctca atcgcctgct ggaactgccc ggcttcatag
     1621 gcggcaatac ccagtagacc gaggctggtg acttccgccg gatcggcttt cagcgcctca
     1681 tcggtcagcg cctgcagttg ttcggtccat tgcttgttgc cggaaaaata cagcgcctgc
     1741 gcccactgcc cgagcagctc cggctcacgc ccggctacac cgacagcgcg ctcgaaggcc
     1801 accgcagcgt cagcggcacg ctcctgagcc atataggtac ggccgaggaa gtaccagctt
     1861 tcggcggact tgggatcgtt ctgcaccgca gcttccaggc gtgcggtcat ttcctcgatg
     1921 gtctttggct gcgcggcaaa ggtttgcgca cgctcgacat catcgatggc gccccagtgc
     1981 tggtacaggc cataccccaa cagaggcatc agcagcgcgg caatcagcgg aatcttgccg
     2041 cccagcgcgc gctgagtcgg ctgcgcttgc gcaccttcgg tatcggcgag caactcgcgg
     2101 gctgcctcat cacggccatt ggccagttgc tcggcattca gcgcacccgc gtcgtgctgg
     2161 ccttgtaggt ccagcaaacg ctcctgatac agggtcacgt taagggcagt acggtcttct
     2221 tcggcctgca ccttgcgcgc acgcagcacc gggatcagca gaaacgccag ggccgtcagc
     2281 aacagcaggc cagcgcataa ccagaattca atcatcagtc tttcttatcc acaggcgttt
     2341 gattaagcag agcagccaga cgctgctgtt catcggcaga caggccactg gcggcggcgc
     2401 ccggcttgct gcgacgacgc accaggatga ccccgagcag gacgaaacca ccggccagca
     2461 aaccagccgg gccataccac agcaacaacg tgcgcgcggt taccggcggt ttgtagagga
     2521 cgaaatcgcc atagcggctg accagaaaat caatgatctg ctcattgctc tggccttcct
     2581 ccagcatgcg aaaaatctgc gcacgcaggt ccatggctat cggcgcatcg gagtcggcaa
     2641 tgttctggtt ctggcactta gggcagcgca gctcttccac caaattgcgg taacgctcgc
     2701 gctcggcctc agtggcaaac tcgtaggtgt cgatagcggc ctgggcggcg cctaacaagg
     2761 ccaaccccag taccgcagcg cttagcagac gcttcatggt tgcacctcat cgaccagctc
     2821 ttggtacagc ggcgccagct gttcacgcca gacgcgctca tcgatcactc cgacaaactt
     2881 gtggcggatt attccgtcct tgtcgatcag gaaggtctca ggcgcaccgt acacgcccag
     2941 gtccaggccc agggtgccgc gcgcgtcgtt gatgttcagc tgataggggt catggaactc
     3001 caccagccac ttctgcgcgg cggcgttgtc atccttgtag ttgaccccgt gaatggtcac
     3061 gccgagctta gccagcttgt tcagcaccgg atgctcaacg cggcaggcca cacaccaggt
     3121 tgcccagaca ttgaccagcg ccggcttgcc cttgaggtcg gcgtcgctca gggtcttgcc
     3181 atcttgcacc gcaggcaggg agaacgccgg gaacggcttg ccgatcagcg ccgaaggtaa
     3241 ctcggtggga tcaaggaaca gcccgcggta gagaaacacc gccacgccga gaaacagcac
     3301 cagcggtaac agcagcagta aacgcttcat gcctgcgccc ccgccatgcc cagtgcatca
     3361 cgcacgcggg tgcgcacctt catgcggtag cgcttgtcgg cggcagcgag gaagccgcca
     3421 aagcccatca ataacgcgcc gaaccagatc cagcgaacga acggtttgat atgcacgcgt
     3481 actgcccagg cgccgttttc cagcggctca cccagagcga cgaacagatc gcgagtgatc
     3541 ccggcatcga taccggcttc ggtcatcacc gactgctgca cggtgtacag gcgcttctcg
     3601 ggatgcagca cggcaatctg cttgccgtct tcgaggatgc gtaccgtgcc tttgtcagag
     3661 gtaaagttcg gtccttcaaa atgctcggca ccttcgaaaa taaactggta gccaccaatt
     3721 tccaccgact cacccggcgc catacgcata tcgcgctcga agctgcccat gctggtcagc
     3781 accacgccca gggcgcacac agccatgccc aggtgtgcgg tctgcatgcc ccagtaactg
     3841 cgggtcaagc ttggcagccc gcggaaaaag cccttgtggc gagtcttctc gacgatgtcg
     3901 cgcagcccgg ccagcaccac ccaggcggcc agcaggcaga caccgagcac cgcccagttg
     3961 aagtcgccga ccaggaaggt ggcgatcacc gccagcacca cgctgccaat cagcaccggc
     4021 gcgagcatgc tcagcagcca ttgcagcggt gtgtccttcc agcgcaccag cacgccgacc
     4081 gcaatcatcg ccatcagcag gcccatcagc ggcacgaaca gcgcgttgaa gtacggcggg
     4141 ccgaccgaca gcttggcccc ggtcatggca tccagcacca gcgggtagag ggtgccgagc
     4201 agaatcatcg ccgcggccac caccagaatc aggttattca ccagcagcag ggtttcccgc
     4261 gaccacaggg caaagccaac ttggcttttc accaccggcg cacgcacggc gaacagcgcc
     4321 aaggagctac ccaccaccaa cagcaagaag gccaggataa acacgccgcg ctcaggatcg
     4381 ctggcgaagg catgcaccga ggtcagcacg ccggagcgca ccaggaaggt ccccagcagg
     4441 ctgagggaga aagcggcaat cgccaacagc acggtccagc tcttgaacac gccacgcttt
     4501 tccgtcacgg ccagggagtg gatcagcgcg gtgccgacca gccagggcat aaaggaagcg
     4561 ttttctaccg ggtcccagaa ccaccagccg ccccagccaa gttcgtagta ggcccaccag
     4621 gagcccaggg cgatcccgat accaaggaag gcccaggcga caatcgtcca cggccgcgac
     4681 cagcgcgccc aggcagcatc aagcttgccg ccgagcagcg cggcgatggc aaaagcgaag
     4741 gccacggaga agccgacata gcccatatag agcatcggcg gatgaatgat caggccgaag
     4801 tcctgcaaca gcgggttgag gtcacggccg tttgccgggg tatcaaacag caggcgctcg
     4861 aacgggttgg aggtgatgat cagaaacagc agaaaaccga tgctgataat gcccatcacc
     4921 gccagcacgc gggcgagcat ctcttccggc aactggcgcg agaagatcga cacggcaaaa
     4981 gtccagccgg cgaggatcag tccccacagc agcagcgaac cttcgtgggc gccccacacg
     5041 gcgctgaact tgtaatacca gggcagcgcg ctgttggaat tgttggccac gtaggcgacg
     5101 gtgaaatcgt cgaccataaa ggagtaggtc aggcagataa aggcgaacag cataaagctg
     5161 aactgccccc aggcggccgg ctgcgccagg ctcatccact gccgatcacc gcgccaggca
     5221 ccaatcaacg gcagggtcgc ctgcaccagc gccatgcaca gcgcgagaat catcgccaga
     5281 tggccgagtt cggggatcat tgcgcgtact ccgatgggga cgcctgcttg gccgctttgg
     5341 ccgcgtcatg tttctgctgc ataccgctct gttccagggc ctgcatcact tccggcggca
     5401 tgtagttttc atcgtgtttg gccagcactt cgtcggcaat caacacgcca ttggcgtcga
     5461 gcttgcccat ggcgacgatg ccctgccctt cgcggaacag gtccggcaag atgccgctaa
     5521 agcgaatggt cacgctcttg gcgccgtcgg tgacgacaaa gtcggtgtcc agcgaatcgc
     5581 tggagcgctt caccgagccc tcttcgacca ggccgccggc gcggatacgg gtgtcctgcg
     5641 gcgcttcgcc attggcgatc tgcgtggggg tgtagaacag gttgatgttc tgctgcaacg
     5701 cactgagggc cagggccacg gcgatgccga caccagcgat gatcgccagg acgatataca
     5761 ggcgcttttt acgcacgggg ttcacttcga ctcctcccgg cgcaaacgac gcgcctcgtc
     5821 ttgcaggtaa cggcgacgcg ccaggattgg cagcgccaca ttcagcccca gcacagccag
     5881 gctgaaggca taacaggacc agacatacgg cccgtgggtg cccatggcga taaagtcgct
     5941 aaagctggca aaactcattg gcctttctcc accaacgcct tgacctcggc tttggcccaa
     6001 ctgctacgcg cctcacgcgt gagcacttcc agacgcatac gcatcagcag caccgcgcca
     6061 aaaaagcagt agaaacccag caccatgatc agcagcggca tccacatctc gaacggcatg
     6121 gcaggcttgg aaaccacgct gaaggtggcg ggctgatgca gggtgttcca ccactccacc
     6181 gagtatttga tgatcgggat attcaccaca ccgacaatcg ccagcaccgc gcaggccttg
     6241 gccgcactgt cgcggttggt gatggcattg cccagggcga tcacgccgaa gtacagaaac
     6301 agcagaatca gcatcgaggt caggcgcgcg tcccacaccc agtaagtgcc ccaggtcggt
     6361 ttgccccaga tagcgccggt caccagcgca atcacggtca tccaggctcc aatgggcgca
     6421 gcctgctgca gggcgacatc ggccagcttc atcttccaca ccaggccgac aatgccggcc
     6481 accgccagca tcacgtagat cgactgagcg agaaacgccg caggtacatg gatatagatg
     6541 atgcgaaagc tgttgccctg ctggtaatcc ggcggcgcaa aggccaggcc ccagaccaga
     6601 ccaaccacca gcagcacggc agcggccagg gccaaccagg ggagccagcg gctgctgatt
     6661 tcataaaacc acttgggcga gccgagcttg tgaaaccacg tccagttcat caggttactc
     6721 atcagtgcta gggttgatca gggcgcaggc aggcacccgg ataaaccggc cattcaggct
     6781 tattgtctac tcagcgacac tgatcgtcag accagcggcg atggcaaaag gtgtcaaggt
     6841 caccgccagt gcggtgaggc tggccaacca caacaggtgg ccgaccgccg gcaatccttg
     6901 caggctggct tgtaacgcac cgctgccgag aatcagcacc gggatgtaca acggcaaaat
     6961 cagtagcgcg agcagcagac cgccgcgctt gaggcccacg gtcagcgccg cgccgaccgc
     7021 gcccagcaag ctcagcaccg gggtaccgag caacagggag atcagcaata ccggcaggca
     7081 acgccccggt aaaccgagca tcagggctaa caatggcgcc agcagcacca gcgccaggcc
     7141 ggagaacagc cagtgtgcca gcaccttggc cagaaccaga agagccaggg ggtgcggcga
     7201 aaggacccac tgctccagcg aaccgtcctc gaaatcactg cgaaacagcc cgtccagcga
     7261 gagcagcacg gccaatagcg ccgctaccca cactagccca ggcgaaaggc tttgtaacaa
     7321 ctgcgtctgc ggccccaccg ccaacgggaa caacgcaatc acaatggcga aaaacaccag
     7381 cgggttggcc agctcggccg gacgacgaaa taacaggcgc gcttcacgag ccagcagcag
     7441 ggtaaaaaca ttgctcatgg cgtgcgttgc cccagatcaa gctcgcggta accggccggc
     7501 ttgaccgtca actcatggtg cgtggtcagc acgaccaggc cgccctgctg acaatgcgcg
     7561 gccagatgac tctccagctg cgccaccgct gccttgtcca acgcggtaaa cggctcatcc
     7621 agcacccaga gtggtggccc aggcagatac agacgggcca acgccacacg gcgctgctgc
     7681 ccggcggaca gcgtgtgaca gggcacatcc tcaaaaccac gcagccccac cgccgccagc
     7741 gcctgccaga tggcctcacg ctcggccggc tgatgcaggg cgcagagcca ggtgaggttc
     7801 tcctcggcag tgagcaagcc cttgataccg gcggcatggc cgatccacaa caggttgcgc
     7861 gccagctcgg cgcgctgcgc ggccagcggg cggccattga ggcgaatctc accagcggta
     7921 ggctgcatca atccggccag caaacgcagc aggctggtct tgccgctgcc gttggggcca
     7981 gccacctgca acatctcacc gggaaccaga cgcagctcaa gacgctcgaa aagcatgcgc
     8041 cagtctcgct cacaggcgag cgcaacggct tcgagaagag gactggtcaa ggcatcggta
     8101 cctaagatgg atggcaaaag ctgttcaagt cggcagagtc ttggccgcta tactgacgca
     8161 agtggaatgc gcagccgaac cagacggggc gcgattatac atgcccaacc tcgcctcccg
     8221 cagtgggcat tttcgacagg gtgtaacccg ccaatgagct aaatcagcag cccacggcct
     8281 attgcgccaa cgccccccag cactcgcccc aacgccatag cggctgacct ggcagtgaaa
     8341 ctgttgcagc ccatgcaagg cctgctggcc agcggtgaaa ccgccaaggc cgaagtcatt
     8401 gccatgaagg aagtggcaca aagctttcag atgctgctca aactgacgtt gaacaacggc
     8461 agccagacca ctctagaagc cagtagcccg cgccccctgg cccaaggcac ctccctggcc
     8521 gtaaccgccc tgtcggaaac ccgcctggcc ctgtccgtac tgatgggtgg tgacaagtcc
     8581 ctgaccagcc tggacctcga ccagctgccg gttggcaccc tactacaggg caaggtggtg
     8641 gcgcgcgagc aactgctgca gggccgctcg cagcaggtga tctacaaggt gctggtcagc
     8701 ctgctcaaca cgccgctggc tggcagcaag ctgtctctgg aaactccgct ggcgctgccc
     8761 ttgggcagcc tgctcagcgc ccaggtacag ggcagccagg cgctaaactt cctgccgctg
     8821 agcggccgtc tcgatcaact cgcgctgagc cagcagctgg gcaaccaaag caaccgccaa
     8881 ggctccctgg aaggtctgtt cagcgccctg caagggctgc gcggcaatgc cgaaatgggt
     8941 gaaagcctgc gcaccagcat cgacaaactc ttcggcgcca tgcccgacgc cgctcaactg
     9001 agcagcccta aaggcctggc catggccctg gaaaacagcg gcatcctgct ggaaagcaaa
     9061 ttgctcggtg gtcagggcca ggcactgccg accgatctga aagccaacct gctgcgcctg
     9121 atcacccaat tgctgcctaa cctgtcagcc ggcacaccac tggccgcggc caatgcaggt
     9181 tcagccctgg cccaggcgat gccggccctg gcccgcgacc tgctcggcag cctgggcaaa
     9241 gccaccagcc gccagcaagc gctgaatttc cctctaccct cgcgcctgct acaggcgatg
     9301 gaaggtgagg ccgatctgga aaccttgctg aagctcgccg ctgcagccat ctcacgccta
     9361 cagacccacc agctctccag cctggcgcag agccaggtcg gcccggacgg caacctgctc
     9421 accacctggc aattggaact gcccatgcgt aaccagcagg aactggtgcc gttgcaggta
     9481 aaaatccaac gcgatgaaag caacccgcaa gacaaacccg agaaaaagga agtcctgtgg
     9541 aaggtcgagc tggccttcga cctcgatccg ctggggcctc tgcaagtgca ggcgcaactg
     9601 gcccacggcc gcttttccag ccagctatgg gccgaacgca gcagcactgt cagcctgatc
     9661 gatgcggagc tggacaacct gcgccagcgc ctgaacgccg ccggcgtaca ggttggcgag
     9721 ctggcctgta gccaaggcac accgccgcgc gggccgcgca cgactctaga acaacgctgg
     9781 gtggatgaaa ccgcatgaag aagaccgccc cccgccaagc catcgccctc acctacgacg
     9841 gccagaatgc gcccgtgctc agcgccaaag gtgatgacga gctggccgaa gccattctcg
     9901 ccatcgcccg tgaatacgaa gtgccgatct acgaaaatgc cgagctggtg cgcctgttgg
     9961 cgcgactgga actgggcgat gcgattcccg agcagctgta tcgctgtatc gcggaaatca
    10021 tcgcctttgc ctggtacctg aagggtaaag cgccggccgg cagtgagcgt gatagcgata
    10081 tcacaccgcc gctgcgccta ttggaagggc cggcacactg aatattgttt gaggatttga
    10141 aaaatcagga acgctgcagt ttggcctgct gcatcttgct caccaactca gcttcagcct
    10201 tgctcaggcc gcaggactgg gtcaggtcat ccacactggc gcccatgcca gccaggcgcg
    10261 cagcctgggt aaacgacaga ctgctggggt cacgctgctc gatctgattg agcttgtccg
    10321 gtagcggcgc gacaacccgt cgcaactcat gcaactcctc gcccatctgc acgctaccag
    10381 tcaggtaggc atccagccgc cggctaagct ccttgagccg ccggtcacgc accgcatcac
    10441 gctcgccttg cagttttatg ctgtcgcgca gctgccgggt cagccagacg catgcgccta
    10501 tcagccccag acagaccaaa accagcgcga tcagcagcgc atcgagcatg gcttagatgc
    10561 tctccagatc ggaccactct tcttcgctca tcatcttgtc cagttcgacc agaatcagca
    10621 gctcgccgtt cttgttgcat acgccctgaa taaacttggc agactcgtcg ttgccaacgt
    10681 tcggcgcggt ctctacttcc gattggcgta gataaaccac ttcggcaacg ctatcgacca
    10741 aaatgccaac tacttgtttg tctgcctcga taatcacgat acgggtgtta tcggtcacct
    10801 cagtggagtc caggccaaaa cgctgacgcg tgtcgatcac cgtcaccaca ttaccgcgca
    10861 ggttgatgat acccagcaca tagctcggag cacccggtac aggggcaatt tcggtatagc
    10921 gcaagacttc ctgaacctgc atcacattga tgccataggt ttcgttgtcc aggcggaacg
    10981 ttacccactg caggatcgga tcatcagaac cttgtgcaga acttttcttc atgtccctag
    11041 cctcgtcatg aaccgctcgc ggcggcaatc gcgggtgtat accccgctgt gattactata
    11101 gatgcaaagc tgcgcacatc gcacagctcg gcgttattca atgtatctgg ctggcggcca
    11161 tgcgcttagc cgcaccactg gcaatcaact ccgccaggga tgcgacatct agcaaggcgc
    11221 acatatgttc gataaccgta cccgccagcc acggacgctg cgtgcgctga ctgcgccatt
    11281 tgacctcgct ggggtcgaga cgaatcgagc gactgacctg atgcaccgcc agcccccatt
    11341 catagccttc aacggaaatc acatactgca aaccttcgcg aaaatcatcg cggtaacgat
    11401 ccggcatcac ccagcgcgcg gtatccagca ctttcaggtt accggcctgg cacggcagta
    11461 tgccgagaaa ccagtctggc tggccaaata atggtgtgag ctcctggcct t
//
LOCUS       DASRUM010000259        21348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1964489, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000259 DASRUM010000000
VERSION     DASRUM010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 21348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21348
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1964489"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1642
                     /locus_tag="VFF22_17555"
     CDS             128..1642
                     /locus_tag="VFF22_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HZX18661.1"
                     /translation="MPKPAPYPRTLTDWLKQLDSQLLPASIDSQQKLRRALADSNRSM
                     RELADLMQGCPALALSVLREANRKSSGLSEQTESLEAAISRLGIKRTEDLLNALPALP
                     EQDLPKALRQIQLISQHASHQANGLFAGRLARLWQEVHWGSLLFLAPIWTLLAAHPEL
                     FEVWEQRVLVKGEAASKVEQELLGVPLLKLCLALSEQWNLPEWVTQGYRLLVSDRRLL
                     VKALHIARDNEHPLHQQQVLDADSNLRRWLTQPANSILLANGLALSAHYAWNSPHSLR
                     WQRLTGLFLQLPLDNVQQLLHQNAVSSARQMPGTDLWHPAEALLWPWQARHLRVTVEQ
                     PKAAVVSEWRQQCAQLLAQPSAFTNVLQLTACANQAIQACGMHRVLILLADRNHTRLI
                     AQQQSGLDKAAASLSLDPQQSQVLRRLLSAPAQLKLSPANIAQFSAMLPGSLKSLFPS
                     EHLLIRSIASNNRVVMLILADQGGQALSDASVQGFGKTVQCIERALTSFANRGR"
     gene            1687..2502
                     /locus_tag="VFF22_17560"
     CDS             1687..2502
                     /locus_tag="VFF22_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020293641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZX18662.1"
                     /translation="MTAFAELSLLIEPAQLASRLDAPELILVDLTSSARYAAGHIPGA
                     HFVDPKRTQLGLPPAPGLLPEQAQLEQLFGELGHNPDAVYVVYDDEGGGWAGRFIWLL
                     DVIGHQRYHYLDGGLQAWLAEERELSTVVPNTGAQAVALQLHAAPIATREYLQSRLGA
                     ADLAIWDARAPSEYSGEKALAQKAGHIPGAINFEWTAGMDPARALRIRQDIQAQLNSL
                     GITADKEIITHCQTHHRSGFTYLVAKLLGYPRVKAYAGSWGEWGNHPDTPVER"
     gene            2537..3397
                     /gene="asd"
                     /locus_tag="VFF22_17565"
     CDS             2537..3397
                     /gene="asd"
                     /locus_tag="VFF22_17565"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003186449.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HZX18663.1"
                     /translation="MKDRLFILSQYLLPHHLLSRLIGCAAECRAGWFKNRLIGWFAKQ
                     YQVNMSEAQVEDLTAFAHFNDFFTRALKDGARPLDSTPGAVLSPADGAVSQLGKIEQG
                     RVFQAKGHSFSVIELLGGDTERAAAFMGGDFATIYLSPKDYHRVHMPLAGTLKEMVYV
                     PGRLFSVNQTTAENVPELFARNERVVCLFDTERGPMAVVLVGAMIVASIETVWAGLIT
                     PPKRALKTFSYDEAARAPIVLEKGAELGRFKLGSTAIVLFGPEQVQWAEELTANSPVQ
                     MGQRLAEPRG"
     gene            3630..5435
                     /locus_tag="VFF22_17570"
     CDS             3630..5435
                     /locus_tag="VFF22_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HZX18664.1"
                     /translation="MDKQNSHLLLRVTTPDKQSLSFCEASPRDLKKWVNGLPKANIGE
                     TARQLYQALVELNQLLTPSENRLQLLEILRPEIYFVCKQLEKHFLNQAIVLDERPRKV
                     ANLCQALQNHLAIGYKLIVARLAAQPGRERHQLLAVALQRATHSLCGPLIRASQLYCP
                     VPEGLWLELHQLYQIALARGLQKTMVRDSQALRTQGLSTEQSYLVALLLGCARCNQMR
                     QSAIARLAEVLEPWSALATLQAGDAPSSLFAIAPQLDGPPRYISLFQQSELHKAVGID
                     PSQLVDAIKEHLLLPAENLAQSRLAVPEGFSHDMLQHLAAAWGDIAERTFQRSQGQGA
                     LTLCIGMSALHFYLAGKRPFNEVLQQPVTTNAAVFKPDTGAPDVWSNAFDAQKSNQWE
                     SGLPFEEIQYNKPHQPNAPAEPSGQESYPTYEVTIVNISPGGYCLSWPKDVPSQLQAG
                     ELLGVQGLPQQGWSIAVVRWIRQVRGGGTQMGIELIAPHAQACGLQLVRKAEQNSQYL
                     RALLLPEISAISRPASLITARLPFQEGNKVSININGNEHRAVLSRRQTSTGSFNQFEY
                     RGVEQISSEHGKPVTAPKSRTPGGEEDFDSLWKSL"
     gene            5500..7569
                     /locus_tag="VFF22_17575"
     CDS             5500..7569
                     /locus_tag="VFF22_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZX18665.1"
                     /translation="MAIEKKTIRLLILEDSQNEAERLVSLFRNAGRATRVHRLSSSDD
                     LNEALQQSWDLLICAPSSEQLAPSEAIGAIRRQAKDIPVIQLLADNDSDSVTEALMLG
                     AQDALPQGEDERLVLVAKRELINLEERRARRASEVALREAEKRCQLLLDSSVDAITYV
                     HDGMHIYANRAYLELFAYEDADELEGMPMIDLIAGCDQVNFKDFLKNYQSAEGSAELS
                     CTGIKANGQSFPAQMSFSPATYDGEPCIQVVIRAETGNAELEEKLREISSQDLVTGLF
                     NRTHFLELMDSAAERAVNAGQPSSLAYIRVDRYASLLADIGLAGIDILLTDLANLLRA
                     HFSADAQLARFGDDVFAVLQPGVNPESTREQLNSLLKKVESHLFDVSGRTVQSTLSIG
                     VAGLNEKTAKAQEVVDRAHRCADELSDGNAIKVFNPADELAAAASRGNIVAMIQQALE
                     KNSFRLLFQPIISLRGDSFEHYEALLRLISPQGEEIPPAELFSAAKDAGLAEKIDRWV
                     ILNSIKLLADHRSKGHNTRLFVHLSSASLQDPTLLPWLSVALKAARLPSDSLVFQFSE
                     PDAIAYLKQAKAITQGLNELHCKTALTQFGCSLNPFNTLKHLHIDFVKVDSSFSQDLS
                     KAENQEALKTLLASLHSQAKLTIVPFVESASVLATLWQAGVNYIQGHYLQGPSQSMDY
                     DFSAGDE"
     gene            complement(7622..8836)
                     /gene="serB"
                     /locus_tag="VFF22_17580"
     CDS             complement(7622..8836)
                     /gene="serB"
                     /locus_tag="VFF22_17580"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HZX18666.1"
                     /translation="MREIVLINITGEDRPGLTAAITGVLAQGGVNILDIGQAVIHDTL
                     SFGILVEIPDTERASSVLKDILFTAYKLDQQVRFTPVSEADYQQWVSGQGKARHIVTL
                     LTRKVTAEQLQRVSAITARYDLNIDHIDRLSGRMPLDMPAEQGKGCIEFSVRGEPADP
                     AALRAEFLSVAQELNVDIAFQRDSLFRRNRRLAVFDMDSTLIEAEVIDELAKAAGVGE
                     QVSEITERAMRGELDFAASFRERLALLKGLPEGVLADIGASLRLTEGAETLFSELRRL
                     GYKTAILSGGFTYFAKQLQAKLGIDYVFANELQIVDGLVTGVAVEPIVDAQRKADLLR
                     ELAAKEGLRLEQTIAVGDGANDLPMLAIAGLGVAFRAKPLVKQSAKQAISTLGLDGIL
                     YLLGFRDRDGLE"
     gene            9011..10552
                     /locus_tag="VFF22_17585"
     CDS             9011..10552
                     /locus_tag="VFF22_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AhpA/YtjB family protein"
                     /protein_id="HZX18667.1"
                     /translation="MNRPTPVKPDNFFLLLFQALRQRRIPLALRIVSHSLLLVALALV
                     IYGWVIGMQFKHAMQQQAEALGTSLITQTAASATELLVSNDILSLNVLLNNLVKNPLV
                     AHAAIYSVDNRILAEAGSRPSQSMLGETEGLYSTPITFQEVIAGQLRISLDMQQFQQP
                     MTISLQSMGILSLILLALTLSLSMRLGRHISTPLLQLRVWLRDPDDPAPGAGRQDEIG
                     DLARQLLARLVPEKPALPEEDEEPFYDDEPVAQDDDDYLPESFDDEPDFEVRDLRDER
                     FDKQNEPVESLDDGAEPYADSTVDDPFSELRDDDEHVPVAAAVPAPQQPQPSAVLAIQ
                     LGAQEQLRRLPRSRLMELLQRYRDCLDRAASLYQGELHTLSDGSSLMLFHQLEIGEDY
                     LTHAICCGELMRALGHALQIEVADSGITLQLQLGLTLGENLSDLSQGDLLLSETAQNA
                     LALSQHSRNLLLVERSIADDALVSQRARIRAIASPQGACCVERLLEPYPSLLERQLAR
                     MHESN"
     gene            complement(10601..11302)
                     /locus_tag="VFF22_17590"
     CDS             complement(10601..11302)
                     /locus_tag="VFF22_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003221076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HZX18668.1"
                     /translation="MTALRFPGVLLLAGVLGLAGCTRYQQAPLYQLDSGEIRMPAAEQ
                     GIAVLLGPVVIADYLQREALLQRQTDGSLTAAEDARWAGSLAADIDQQLLRQLASRLD
                     SQRLALAPAAAGFVPQVRLGLSITRLDSGPQRPAVLEAQWRLTGTDGKLLDGRLVRLE
                     EAHSGTIVDQVRAQSRLVQQLVEQLAVAVETHGKPAAAVPAPPRKSAPVKPAPSTPRI
                     PVFEPMRDEGEVFRF"
     gene            complement(11456..13714)
                     /gene="parC"
                     /locus_tag="VFF22_17595"
     CDS             complement(11456..13714)
                     /gene="parC"
                     /locus_tag="VFF22_17595"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HZX18669.1"
                     /translation="MSESLDLSLDGVERRSLADFTEQAYLNYSMYVIMDRALPHIGDG
                     LKPVQRRIVYAMSELGLDADSKHKKSARTVGDVLGKFHPHGDSACYEAMVLMAQPFSY
                     RYTLVDGQGNWGAPDDPKSFAAMRYTEARLSRYSEVLLTELGQGTVDWVPNFDGTMNE
                     PATLPARLPNILLNGTTGIAVGMATDVPPHNLREVATACIRLLDEPNATVEQLCEHIL
                     GPDYPTEAEIITPRSDLLKIYETGKGSVRMRAVYRVEDGDVVVTSLPHQVSGAKVLEQ
                     IAAQMQAKKLPMVADLRDESDHEHPCRIVIIPRSNRVDADELMQHLFATTELESSYRV
                     NTNVIGLDGKPSVKDLRTLLTEWLQYRVGTVRRRLQFRLDKVEHRLHLLEGRLIAFLN
                     LDEVIRIIRTEDSPKPVLMERFGLTEIQAEYILETRLRQLARLEEMQIRGEQEELAKE
                     RDKLLALLGSETKLKKLVRQELLADAEKYGDDRRSPIVARAEAKALSETELMPTEPVT
                     VVISEKGWVRCAKGHDIDATGLSYKAGDGFKTAAPGRSNQYAVFIDSTGRSYSLAAHS
                     LPSARGQGEPLTGRLTPPPGATFECVLLPDDSALYVIASDAGYGFVVKGEDLQAKNKA
                     GKALLSLPTGALVVPPKPLANREEDWLAAVTTEGRLLLFPVRDLPQLGKGKGNKIIGI
                     PGERVASREEYLTDLAVLPVGATLVLQAGKRTLSLKADDLEHYKGERGRRGNKLPRGF
                     QRVDSLLVEFQG"
     gene            complement(13722..14261)
                     /locus_tag="VFF22_17600"
     CDS             complement(13722..14261)
                     /locus_tag="VFF22_17600"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HZX18670.1"
                     /translation="MSEQPAGKRAGRVMLVLAWGAALLLATKFFGDWEDAQRNPNQMP
                     ESLHGSGYVEVHLASSRQGHYMAGGKINGEEVTFLLDTGATQVAVPIEVAQRLGLQAG
                     AAITINTANGRATAHRTRLERLQLGDIVLTDVEALIAPGMGGDDVLLGMSALKQLEFT
                     QRGGTLMLRQTTSQQPTLQ"
     gene            complement(14258..15241)
                     /locus_tag="VFF22_17605"
     CDS             complement(14258..15241)
                     /locus_tag="VFF22_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HZX18671.1"
                     /translation="MQRLLAVCLLLCSPLLASAAVLEELKLLNEQAVSGVSGGNLSGL
                     AWCDNALWAVSDRDDQQLYRLESDESQWQATAETFVAPPAPDARLPWGLRMRSWAASL
                     VRGGELDFEGISCDAAGNRFLVSEAKAAVLQLPVIGEPNWLKLPQGLLRQARASGMLL
                     HYNSLFEGIAVDPAGERLYLAAERMRRGLLVAHKQQAIWRCTGGCVLFSEAGTEPAPE
                     QLGAKEQPRDFSGLAFHNEKLFTLERQAHRICRRSLSNGQVEKCWSFAAEALTPERLY
                     PPSYGMAEALWVDQDGAWVGVDNGRFSRADGESRPIVWRFAGPKGGWGSKP"
     gene            complement(15241..17106)
                     /gene="parE"
                     /locus_tag="VFF22_17610"
     CDS             complement(15241..17106)
                     /gene="parE"
                     /locus_tag="VFF22_17610"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016395266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HZX18672.1"
                     /translation="MEVLSGLDPVRKRPGMYTDTSRPNHLAQEVIDNSVDEALAGHAK
                     SIQVILHEDNSLEVLDDGRGMPVDIHPEEGVPGVELILTKLHAGGKFSNKNYQFSGGL
                     HGVGISVVNALSTSVIVTVKRDGNEYQMSFADGYKASDLAVVGTVGKRNTGTSVHFWP
                     DAKYFDSFKFSVSRLKHVLKAKAVLCPGLAVSFEDKASGEKVEWMYEDGLRSYLVDAV
                     SEFERLPNEPFCGSLAGNKEAVDWALLWLPEGGDAVQESYVNLIPTAQGGTHVNGLRQ
                     GLLDAMREFCEFRSLLPRGVKLAPEDIWERIAFVLSMKMQDAQFSGQTKERLSSREAA
                     AFVSGVVKDAFSLWLNGHPELGQQLAELAISNANRRLKAGKKVERKRITQGPALPGKL
                     ADCAGQDPMRSELFLVEGDSAGGSAKQARDKEFQAILPLRGKILNTWEVDGSEVLASQ
                     EVHNIAVAVGLDPGSSDLSQLRYGKICILADADSDGLHIATLLCALFVQHFRPLVDAG
                     HVYVAMPPLYRIDLGKEIFYALDEAERDGILDRLVAEKRRGKPQVTRFKGLGEMNPPQ
                     LRETTMDPNTRRLVQLTLDDYPGTQEMMDMLLAKKRAGDRKSWLESKGNLAEVLL"
     gene            complement(17161..17772)
                     /locus_tag="VFF22_17615"
     CDS             complement(17161..17772)
                     /locus_tag="VFF22_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HZX18673.1"
                     /translation="MTTSILYIHGLNSSPASLKASQLQRAMAQLGLDAQLRVPGLHHH
                     PRQAIAQLEALIAELGRPVLVGSSLGGYYATHLAERHGLSALLINPAVRPHLRFDGYL
                     GPQKNYYSDETWELTADHVTALAELDVLALQDPARYQVWLQTADETLDYRDAQRYYRA
                     CALRIQAGGDHGFQGFAERLPMLFAFAGIPRHLWQDIDFSALN"
     gene            complement(17828..18649)
                     /gene="cpdA"
                     /locus_tag="VFF22_17620"
     CDS             complement(17828..18649)
                     /gene="cpdA"
                     /locus_tag="VFF22_17620"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HZX18674.1"
                     /translation="MPGAPTKTTAVDHSVLLVQLSDSHLFADEAGKLLGMDTQDSLQR
                     VIERVLQEQPQIDLILASGDLSQDGSAESYQRFREMTAAIPAPARWFPGNHDEVPAMQ
                     AACVGSDLLEPVIDLGNWRVTLLDSSIPGAVPGYLSEQQLTLLERALSEVPDRHHLIC
                     LHHHPVSIGCKWMEPIGLRNPEALFAVLERFSQVRAVLWGHIHQELDQQRGPIRLLAS
                     PSTCVQFAPGSEEFQVDKTAPGYRWLRLFDDGRLETGVSRVTGIKFEIDYTIKGY"
     gene            complement(18823..19281)
                     /locus_tag="VFF22_17625"
     CDS             complement(18823..19281)
                     /locus_tag="VFF22_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003377111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HZX18675.1"
                     /translation="MVMNLLRERYRVDLVELQTACELNYVRLLQLLPDMRGEGLQQVR
                     RVALSQGEHLLGVLALEVLETCPYTSTIEVRQEHSLSWLPVPRLEVRVYHDARMAEVV
                     GTQSARRFHGIYPYPNAAMHQPDEKTQLNLFLGEWLSHCLACGHELAPVR"
     gene            complement(19272..19871)
                     /locus_tag="VFF22_17630"
     CDS             complement(19272..19871)
                     /locus_tag="VFF22_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZX18676.1"
                     /translation="MQRDDVEIIERESCFSGFYKLDRLRLRHRQFAGGMGPELSRELF
                     VRHDAVCVLPYDPQRDCVVLIEQFRVGAMEKSANPWLMELVAGLIDKDEQPEEVACRE
                     AMEEANLPLTSLWPITQYYPSPGGSDERVHLFVGRCDSEGAGGVHGLAEEGEDIRVHV
                     WPLEDALDAVKDGRIDNAASIIALQWLALNRAEVRGLWS"
     gene            complement(19961..>21348)
                     /gene="thiC"
                     /locus_tag="VFF22_17635"
     CDS             complement(19961..>21348)
                     /gene="thiC"
                     /locus_tag="VFF22_17635"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HZX18677.1"
                     /translation="HRAAGLLDQQHPGHSFGASIPKEITAEFVREEIARGRAIIPANI
                     NHVELEPMIIGRNFLVKINGNIGNSALGSSIEEEVAKLTWGIRWGSDTVMDLSTGKHI
                     HETREWIIRNSPVPIGTVPIYQALEKVGGAAEELTWELFRDTLIEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEDICEIMKAYDVSFSL
                     GDGLRPGSIADANDEAQFGELETLGELTKIAWKHDVQTMIEGPGHVPMQLIKENMDKQ
                     LECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKD
                     DVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLGLDPDTARSYH
                     DETLPKDSAKVAHFCSMCGPKFCSMKITQEVRVYAEEQRIAALDLDAEAGMQAKAAEF
                     KAQGAQLYHKV"
BASE COUNT         4323 a         6886 c         6257 g         3882 t
ORIGIN      
        1 tttgccatag aaagagactt gccagagagg gtagaaaggg gctttgcaaa acaacccttc
       61 tatttatcgg aagttttgcg cgagactata gccagttaag gtcaaaagcc agcttgtctc
      121 aacccgcatg ccaaagcccg caccctatcc gcgcaccctg acagactggc tcaagcagct
      181 ggacagccag ctattgcctg cttccatcga cagtcaacaa aaactgcggc gtgccctggc
      241 ggacagtaat cgctcgatgc gtgagctggc agacctgatg cagggctgcc cagcgctggc
      301 gctcagcgtc ttgcgtgaag ccaaccgcaa gagcagcggc ctgagcgagc aaaccgaaag
      361 cctcgaagct gccatcagcc gcctcggcat caaacgcacc gaagacctgc ttaatgccct
      421 gcccgccctg cccgagcagg atttacctaa agccctgcgg caaattcagc tgatcagtca
      481 gcatgccagc caccaggcca acggcctgtt tgccggccgc ctggcgcggc tgtggcagga
      541 agtgcactgg ggcagcctgc tgtttctcgc accgatctgg accttgctcg ccgcccaccc
      601 tgagctgttc gaggtctggg agcagcgtgt actggtcaag ggcgaagccg cgagcaaggt
      661 agagcaggag ttgctcggcg taccgctgct gaaactctgc ttggccctga gcgagcaatg
      721 gaatttgccg gagtgggtta ctcagggcta tcggctgctg gtcagcgacc gacgcctgct
      781 ggtcaaagcg ctgcatatcg cccgcgacaa tgaacatccc ctgcatcagc aacaagtcct
      841 cgacgccgac agcaacctac ggcgctggct gactcaaccg gccaacagca ttctgctggc
      901 caacggcctg gcgctgtccg cgcattatgc gtggaacagc ccgcacagcc tgcgttggca
      961 gcgcctgacc ggtttattcc tgcagctgcc gctcgataac gtgcagcagc tgctgcacca
     1021 gaacgccgtt agcagcgccc ggcaaatgcc cggcaccgac ctttggcacc ccgccgaagc
     1081 gctgctctgg ccgtggcagg cgcggcacct gcgagtcaca gtcgaacaac ccaaggcggc
     1141 ggtcgtcagc gaatggcgcc agcaatgcgc gcaactgctc gcacaaccca gtgcgtttac
     1201 caacgtgctg cagctcaccg cctgcgccaa tcaggccatc caggcctgcg gcatgcaccg
     1261 ggtactgatc ctgctcgctg atcgcaacca cacccgcctg atcgcccagc agcagagcgg
     1321 cctggacaaa gccgccgcca gcctcagcct tgacccgcag caaagccagg tgctgcgccg
     1381 cctgctcagt gccccggcac aactcaaact gagccctgcc aacatcgcgc agttttccgc
     1441 gatgctacct ggctcactga aaagcctgtt ccccagcgaa cacctgctga ttcgctcgat
     1501 cgccagcaac aaccgcgtgg tcatgctgat cctcgccgat cagggcggcc aggcactgag
     1561 tgatgccagc gtgcagggct tcggcaaaac cgtgcaatgc attgagcgcg ccctgaccag
     1621 ctttgccaac cgcggtcgct aggctttccg ctacaatccg tcccttcccg ccaaaagagg
     1681 cccggcatga ccgccttcgc tgagttgtcc ttgctgatcg aacccgcgca actggccagc
     1741 cgcctggatg cgcccgagct gatcctggtt gacctgacca gcagcgcccg ctacgccgcc
     1801 ggccacatcc ctggcgccca ttttgtcgac cctaaacgca cccagctggg cctgccgcca
     1861 gcacctggtt tgctgccgga acaggcacaa cttgagcaat tgttcggcga gctggggcat
     1921 aaccccgatg ccgtgtatgt ggtgtatgac gatgaaggcg gcggctgggc cggacgcttt
     1981 atctggctgc tggatgtgat cggccatcag cgttaccact atttggacgg cggcctacag
     2041 gcctggctgg cagaggagcg cgagctcagc acagttgtgc cgaataccgg cgcacaggct
     2101 gttgccctgc agctgcatgc agcaccgatc gccacccgtg aatacctgca aagccgcctt
     2161 ggcgccgccg acctggccat ctgggatgcc cgcgccccca gtgaatacag cggtgagaaa
     2221 gccctggcgc aaaaagccgg gcatatcccc ggagccatca atttcgaatg gactgcgggc
     2281 atggatccgg cgcgggcgct gcgcattcgc caggacatcc aggcgcagct caatagcctc
     2341 ggcatcacgg ccgacaaaga aatcatcacc cactgccaga cccaccaccg ttccggcttt
     2401 acctacctgg tcgccaagct tctcggctat ccacgggtaa aagcctacgc cggctcctgg
     2461 ggcgagtggg gcaaccatcc ggataccccg gtcgagcgtt aagccgcaga ccgccagcca
     2521 cttttaagga acgccaatga aagatcgcct gtttatcctc agccagtacc tgctgccgca
     2581 tcacctgctt tcgcgcctga tcggctgcgc cgcggaatgc cgcgccggtt ggttcaagaa
     2641 ccgcttgatc ggctggttcg ccaagcagta ccaggtcaac atgagcgaag cccaggtcga
     2701 agacctgacc gctttcgccc acttcaatga cttctttacg cgcgccctga aagacggcgc
     2761 ccgcccgctc gacagcacac ctggcgcggt gctcagcccc gccgatggcg cagtcagcca
     2821 actcggcaaa atcgaacaag gtcgagtatt tcaggccaag ggccacagct tcagcgtgat
     2881 tgagctgctg ggcggcgaca cggagcgtgc cgcggccttt atgggcggcg atttcgccac
     2941 catctacctt tcacccaagg actaccaccg cgtgcacatg ccgctggctg gcaccctgaa
     3001 agaaatggtc tacgtgccag gccggctgtt ctcggtcaat cagaccacag cggaaaatgt
     3061 acccgagctg ttcgcgcgca acgagcgcgt ggtctgcctg ttcgacacag aacgcggccc
     3121 gatggccgtg gtactggtcg gcgcgatgat cgtggcctcg attgaaaccg tctgggcagg
     3181 cctgatcacc ccaccgaaac gcgcgttgaa aacattcagt tacgacgaag cagcacgtgc
     3241 accaatcgtg ctggaaaaag gtgcagagct cgggcgcttc aaactgggct ccaccgccat
     3301 cgtgttgttt ggcccggaac aggtgcagtg ggccgaagaa ttgaccgcta acagcccggt
     3361 acagatgggc cagcgcctcg ccgagccacg cggctaacac gccaacccgg cactgtcagc
     3421 gtacacaagc agtgccggag cgacggccta gagtgaaccg ctgagcagaa aaccagtcac
     3481 aacaatgact tccagatggt ttgtatgccg tgccagcttg gcaacccagc caaggacatg
     3541 gctgcggggg gcgcgagatg ctgctagagt cgaaacataa ttcctagact cacctgcctt
     3601 gcccgtgcgc tgtggagtat ccgacttaga tggataaaca gaattctcac ttgctcctgc
     3661 gtgtcaccac gccggataag caaagcctgt cattctgcga agccagtcct cgtgatctga
     3721 aaaagtgggt caatggtctg cccaaagcca atatcggtga aaccgcacgc cagctttacc
     3781 aggcattggt tgaacttaac caactgctga ccccctcaga aaatcgcctg caactgctgg
     3841 agatcctccg cccggagatc tatttcgtct gcaagcaact ggaaaaacat ttccttaacc
     3901 aggccatcgt ccttgatgaa cggccgcgta aggttgccaa cctctgccag gccctgcaaa
     3961 accacctggc catcggctac aaactgatcg tcgcccgcct ggccgcacaa cccggtcgtg
     4021 agcgccacca gctgctagcc gtcgccctgc agcgcgccac tcacagccta tgtggcccgc
     4081 taattcgcgc cagccaactg tactgccctg ttccagaagg tctctggctg gagttgcacc
     4141 agctgtacca gatagccctg gcccgcggcc tgcaaaagac catggttcgt gacagtcaag
     4201 ccctgcgcac ccaaggttta agcaccgagc aaagctatct ggtcgctctg ctactcggtt
     4261 gcgcgcgctg caaccagatg cgccaaagcg ctattgcgcg cctggccgaa gtgctcgaac
     4321 cctggagcgc gctagcgacc ctgcaagccg gagatgcgcc ctccagcctg ttcgccattg
     4381 cgccgcaact ggatggcccg ccccgttata tctcgctatt ccagcagagc gagctgcata
     4441 aagcggtggg catcgatccc agccagttgg tagacgccat caaagagcac ctgctgctgc
     4501 cggccgaaaa cctcgcgcaa tctcgcctgg ccgtcccgga gggcttcagc cacgacatgc
     4561 tgcagcacct ggccgccgcc tggggcgata ttgccgaacg caccttccaa cgcagccagg
     4621 gccagggcgc cctaaccctg tgcatcggca tgagcgcgct gcatttctat ctggccggca
     4681 aacgtccgtt taacgaagtg ctgcagcagc cggtgacgac caatgctgcg gtattcaagc
     4741 ctgacaccgg cgcaccggat gtctggtcga acgcctttga tgcgcaaaag agcaaccagt
     4801 gggaaagcgg tctgcccttc gaggaaatcc aatacaacaa gccgcatcag cccaatgcgc
     4861 ctgccgagcc cagtggccaa gaaagctacc cgacctatga agtgaccatc gtcaatatca
     4921 gcccaggcgg ctactgcttg tcatggccga aagatgtgcc cagccaattg caggccggcg
     4981 aactactggg cgtacaaggc ctgccgcagc aaggctggag cattgccgta gtgcgctgga
     5041 ttcgccaggt acgtggcggc ggcacccaga tgggcattga gttgattgcc ccgcatgcgc
     5101 aagcctgcgg cctgcagctg gtgcgcaagg ctgagcaaaa cagccagtat ctacgggccc
     5161 tgctgttgcc ggaaatcagc gccatttcgc gcccagccag cctgatcacc gcgcgcctgc
     5221 cgttccagga aggcaacaag gtcagcatca atatcaatgg caacgaacac cgtgcggtgc
     5281 tcagccgcag acaaaccagc accggtagct tcaaccagtt tgaataccgg ggcgtcgagc
     5341 agatcagcag tgagcacggg aaacctgtca cagcgccgaa aagccgcacg ccaggcgggg
     5401 aggaagactt tgactcactt tggaagtcgc tgtagattgc cgacgcactc ccttttgtcg
     5461 ccttttcgcg cccgtttggc gcagatttga taccagacca tggccattga aaaaaaaacc
     5521 attcggctac tgatactcga agactcccag aatgaggccg agcgcctggt cagcctgttc
     5581 cgtaatgccg gacgggccac ccgcgtgcac cgccttagct ccagcgacga cctcaacgaa
     5641 gccctgcagc aaagctggga cctgctgatt tgcgcaccga gcagcgaaca actggcgcca
     5701 agtgaagcga ttggcgcgat ccgccgccag gccaaggata ttccagtcat ccagctgctt
     5761 gcagacaacg actccgacag cgtcaccgaa gcgctgatgc tcggcgccca ggacgcgttg
     5821 ccacaaggtg aagacgagcg cctggttctg gtcgccaagc gcgaactgat caacctagaa
     5881 gaacgccgtg cccgccgcgc ctccgaagtg gccctgcgcg aagcggaaaa acgctgccaa
     5941 ctgctgctcg acagctccgt cgatgccatc acctacgtgc acgacggcat gcacatctac
     6001 gccaatcgcg cctatctgga gctgttcgcc tacgaagatg ccgacgagct ggaaggcatg
     6061 ccgatgattg acctgatcgc tggctgtgat caggtcaact ttaaagattt tctgaaaaac
     6121 taccaaagcg ccgaaggcag cgccgagctc agctgtaccg gaatcaaagc caacggccag
     6181 agcttcccgg cgcagatgag cttctccccc gccacctacg atggcgaacc gtgcatccag
     6241 gtggtgattc gcgccgaaac cggcaacgcc gaactggaag aaaaactgcg cgagatcagc
     6301 agccaggatc tggtcacagg cctgttcaat cgcacccact tcctcgaact gatggacagt
     6361 gccgccgagc gcgcggtaaa tgccggacaa ccgtccagcc tcgcttacat ccgcgttgac
     6421 cgctacgcca gcctgcttgc cgacataggt ttggcgggca tagatatttt gctgaccgac
     6481 ctggccaact tgctccgcgc gcacttcagc gccgacgcgc aactggcgcg ctttggtgat
     6541 gacgtatttg ccgtgctgca acccggcgtc aacccggaaa gtactcgcga gcagctgaac
     6601 agcctgctga aaaaagtcga aagtcacctg ttcgacgtca gcggccgcac cgtgcaaagt
     6661 acgctgtcga ttggcgtagc cggacttaac gagaaaaccg ccaaggccca agaagtagtc
     6721 gaccgcgccc atcgctgcgc cgacgagctg agcgatggca acgcaatcaa ggtgttcaac
     6781 ccagccgatg agctggccgc agcagcaagc cgcggcaata ttgtcgcgat gattcagcag
     6841 gcgctggaga agaatagctt ccgcctgctg ttccagccga tcatcagcct gcgcggcgac
     6901 agctttgagc actatgaagc gctgctgcgc ctgatcagcc cgcagggcga agaaattccg
     6961 ccggctgagc tgttcagtgc agccaaagat gccgggcttg ccgaaaaaat cgaccgttgg
     7021 gtgatcctca actcgatcaa actgctcgcc gaccaccgca gcaaaggcca caacactcgc
     7081 ctgttcgtgc acctttcaag cgccagtttg caagatccaa ccctgctgcc atggctcagc
     7141 gtggccctga aagccgcccg cctcccgtcg gactcactgg tgttccagtt cagcgagccg
     7201 gatgcgattg cctacctgaa acaggccaag gccatcaccc agggcctcaa cgagctgcac
     7261 tgcaaaaccg cactgaccca gttcggctgc tcactgaatc cgttcaacac gctcaagcac
     7321 ctgcatatcg acttcgtcaa agtcgacagc tcgttctccc aggacctgag caaggcggaa
     7381 aaccaggaag cgttgaaaac cctgctggcc agcctgcact cccaagccaa gctgaccatc
     7441 gtgcccttcg tcgagagcgc cagcgtactg gccactctct ggcaggctgg ggtcaactat
     7501 atccaggggc attacctgca gggcccgagc cagtcgatgg attacgattt ctcggccggt
     7561 gacgagtaac aaccggcgaa ataaaaaagc cgcccgaggg cggcttttgt ttacctgtcg
     7621 tttactccag gccgtcgcga tcgcgaaagc ccaacagata gagaatgccg tccaggccca
     7681 gggtcgagat cgcctgtttg gccgattgtt tgaccagcgg tttggcgcga aacgccacac
     7741 ccaggccggc aatcgccagc atcggcaaat cattggcgcc gtcgcccacc gcgatggtct
     7801 gctccagacg caagccttcc ttggccgcca gctcgcgcag caagtcagct ttgcgctgag
     7861 cgtcaacaat cggctcgacc gccacaccag tgaccaggcc atcgacaatc tgcagctcgt
     7921 tggcgaacac atagtcgata cccagcttgg cctgcagctg cttggcaaag taggtaaagc
     7981 cgccggagag gatcgcggtc ttgtaaccca agcggcgtag ctcgctgaac agggtttccg
     8041 cgccttcagt caggcgcaga gacgcgccga tatcggccag aacaccttcc ggcaggccct
     8101 tgagcagcgc caggcgctcg cggaagctgg cggcaaaatc cagctcacca cgcatggcgc
     8161 gctcggtaat ttctgaaacc tgctcaccca cgcctgcggc cttggccagc tcatcgatga
     8221 cttcggcctc gatcagcgtc gaatccatgt cgaacaccgc caagcggcga ttgcggcgga
     8281 acagcgaatc gcgctggaag gcgatatcga cattcaattc ctgcgccacg ctaaggaact
     8341 cggcgcgcag agctgccgga tcagccggct cgccgcgtac ggaaaactcg atgcagccct
     8401 tgccctgctc ggccggcata tccagcggca tgcgcccgga aaggcggtcg atatggtcga
     8461 tattcaggtc ataacgcgcg gtaattgcac tgacgcgctg cagctgctcg gcggtaacct
     8521 tgcgcgttag cagggtgacg atatgccgag ccttgccctg gccactcacc cattgctggt
     8581 agtccgcttc ggacaccgga gtgaaacgca cctgttgatc gagcttgtag gcggtaaaca
     8641 gaatgtcctt gagcaccgac gaggcgcgct cggtatccgg gatctcgacc aggatgccga
     8701 acgacagggt gtcatggatc accgcctggc caatatcgag gatattcacc ccgccctggg
     8761 ccagcacgcc agtgatggca gcagtcaaac cggggcgatc ttcgccggtg atattgatca
     8821 ggacgatttc gcgcaaggct cggtctccag tacagcgtca ggggcagcgg cgtgctgaac
     8881 gggtcacaag ccgcggaaaa ggcgcacatt ctacccattt tcacgggtaa acagccacgc
     8941 acggcgcttt gcccactgcc ggggcgccgc tatactgcgc gccaactaca gtcgacatga
     9001 gccgagctct gtgaaccggc ccacccccgt aaaacccgat aacttcttcc tgctgctgtt
     9061 ccaggccctg cgccaacggc gcatccctct ggcattgcgc attgtcagcc atagcctgct
     9121 gctggtggcc ctggccctgg tgatctatgg ctgggtgatt ggcatgcagt ttaagcatgc
     9181 catgcagcag caggccgaag ccctgggtac cagcctgatt acccaaacgg ccgcctcggc
     9241 gaccgaactg ctggtgtcca atgacatcct cagcctcaac gtgttgctca ataacctggt
     9301 gaaaaacccg ttggtggccc acgccgcaat ctacagcgtg gacaaccgca tcctcgccga
     9361 agccggctcg cgccccagcc agagcatgct cggtgaaacc gaagggctgt attcgacgcc
     9421 gattaccttc caggaggtga tcgccgggca gctgcgcatc agcctggata tgcagcaatt
     9481 ccagcaaccg atgaccatca gcctgcagag catgggcatt ctcagcctga ttctgctggc
     9541 cctgacccta tccctgagca tgcgcctggg gcggcatatc tccacgccac tgctgcagct
     9601 gcgagtctgg ctgcgtgacc cggatgatcc ggcgcccggc gccggccgcc aggatgaaat
     9661 cggtgacctg gcgcgccagt tgctggcacg cctggtaccg gagaaacctg cactgccaga
     9721 agaagacgaa gaaccgttct acgacgacga gccagtcgcc caggacgatg acgactacct
     9781 gcctgaatcg ttcgatgatg agccggactt cgaagtgcgc gacctgcgcg atgagcgctt
     9841 cgataagcag aacgagccgg ttgaatccct ggacgatggc gccgagccct acgccgacag
     9901 cacagtggac gatccattct ccgagctgcg cgatgatgac gaacatgtgc ctgtagctgc
     9961 agctgtaccc gcaccacagc agccgcagcc cagcgccgtg ctggcaatcc agctgggtgc
    10021 ccaggaacaa ctacggcgcc tgccgcgcag tcgcctgatg gagttgctgc aacgctaccg
    10081 cgactgcctg gaccgggctg caagcctgta tcagggtgag ctgcacaccc tcagcgatgg
    10141 cagcagcctg atgctgtttc accagttgga aattggtgag gactacctga cccacgccat
    10201 ctgctgcggc gaattgatgc gtgcgctcgg tcatgccctg cagatcgaag tggcggacag
    10261 cggcatcacc ctgcaactgc agcttggcct gaccctgggt gaaaacctca gcgacctcag
    10321 ccagggcgat ctgctgctca gcgaaacggc gcagaacgcc cttgcgctgt cacaacacag
    10381 ccgcaacctg ctgctggtcg aacgcagcat tgccgacgat gcattggtca gccagcgcgc
    10441 gcgcattcgc gccatcgcca gtccgcaagg cgcctgctgt gtggagcggt tgctggagcc
    10501 ctacccgtcg ctgctggagc gccagctggc gcgtatgcac gaaagcaact aagcgcacgc
    10561 acctgactga accgctcaga caggtgctac ggcttatcgt ttagaaacgg aagacttcgc
    10621 cctcatcacg catcggttca aacaccggga tacgcggcgt ggatggcgcc ggttttaccg
    10681 gcgctgattt acgtggcggt gcgggtactg ctgccgcagg tttaccgtga gtttccacgg
    10741 ctacagccaa ttgctcgacc aactgttgta ccagccgact ctgcgcccgc acctggtcga
    10801 caatagtacc gctgtgggct tcttccagac gcaccagacg cccgtcgagc aacttgccgt
    10861 cagtaccggt taaacgccac tgcgcttcca gcacggccgg ccgctgcgga ccagagtcca
    10921 ggcgggtaat cgacagcccc aaacgcacct gcggcacaaa accggcagca gccggcgcca
    10981 gcgccagacg ctggctatcc agacgcgagg ccaactggcg caacagctgc tgatcgatgt
    11041 cggcggccag gctgcccgcc cagcgcgcgt cctcggcggc ggttaggcta ccgtcggtct
    11101 ggcgctgcaa cagcgcctcg cgttgcaagt aatcggcaat caccaccggc ccaagcagca
    11161 cggcaatgcc ttgctcggct gctggcatgc gaatctcgcc gctatcgagc tggtacagcg
    11221 gcgcttgctg ataacgcgta caacccgcca ggccaagcac cccggccagc agcaaaactc
    11281 caggaaaacg cagtgcagtc atcttactca tccagtcggt aggtctaccg gcgcgcatag
    11341 caaattaggt gcaagtggat catgtcaatc gtccacatta cggccgcata tcatccttga
    11401 aagccgcccg cgactccagc gcggcactgt gccggcatgc gtcaaggcct agcgcttagc
    11461 cctggaactc gaccaacagg ctgtcgaccc gctggaagcc gcgcggcaac ttgttgccac
    11521 ggcgtccgcg ctcacccttg tagtgttcca ggtcatcggc cttgagcgac agggtgcgct
    11581 taccggcctg caacaccagg gtcgccccga ccggcagcac ggccagatcg gtgaggtatt
    11641 cctcgcgcga agcaacccgc tcaccgggga taccaatgat cttgttgccc ttgcctttgc
    11701 ccagttgcgg cagatcgcgc accgggaaca gcagcagacg gccttcggtg gttaccgcgg
    11761 ccagccaatc ttcttcgcgg ttggccaacg gcttcggcgg caccaccagc gcgccagttg
    11821 gcaggctgag caacgcctta ccggccttgt tcttggcttg caggtcttca cccttgacca
    11881 caaagccgta gccagcatcg gaggcgatca catacagcgc actgtcatcc ggtagcagca
    11941 cacattcgaa ggtcgccccc ggcggcggcg tcaggcggcc ggtcaaaggc tcgccctggc
    12001 cacgggcgga gggcagcgag tgcgcggcca gcgagtaact gcgcccggtg gagtcgataa
    12061 acaccgcgta ctggttggag cgacctggcg cggcggtctt gaagccatcg ccagctttgt
    12121 acgagagacc agtggcgtcg atatcgtggc ccttggcgca gcgcacccag cctttttccg
    12181 agatcaccac ggtgactggc tcggtcggca tcaactcggt ttcgctgagc gccttggcct
    12241 cggcgcgggc aacgatcggc gagcgacggt cgtcgccgta tttctcggcg tcggccagaa
    12301 gttcctggcg caccagcttc ttcaacttgg tttcactgcc cagcagggcc agcagcttgt
    12361 cgcgttcctt ggccagctct tcctgctcgc cacggatctg catttcttca agccgtgcga
    12421 gctgacgcag gcgggtctca aggatgtact cggcctggat ctcggtaaga ccgaagcgct
    12481 ccatcagcac cggcttgggc gaatcctcgg tgcggatgat gcgaatgact tcgtcgaggt
    12541 tgaggaaggc aatcaagcgg ccttccaaca ggtgcaggcg atgctcgacc ttgtccagac
    12601 ggaactgcag gcgacggcgc acggtgccga cgcggtactg caaccactcg gtgagcaggg
    12661 tgcgcaggtc ttttaccgac ggcttgccgt ctaggccaat cacgttggtg ttgacccggt
    12721 agctggactc cagctcggtg gtggcgaaca ggtgctgcat cagctcgtcg gcatccaccc
    12781 ggttggagcg cgggatgatc acgatgcggc aggggtgctc gtggtccgac tcatcgcgca
    12841 ggtcggcgac catcggcagc ttcttcgcct gcatctgcgc ggcaatctgc tctagcacct
    12901 tggccccgga cacctgatgc ggcagcgacg tgacaaccac gtcgccgtcc tcgacgcggt
    12961 acacggctcg catgcgcacc gagcccttgc cggtttcgta gattttcagc aggtcgctgc
    13021 gcggggtgat gatttccgct tcggtcgggt agtccgggcc gagaatatgc tcacagagct
    13081 gctcgaccgt ggcgttcggc tcgtccaaca ggcgaataca ggccgtcgca acctcacgca
    13141 ggttatgcgg cggcacgtcg gtggccatac ccacggcgat gccggtggtg ccgttaagta
    13201 gaatattcgg caggcgcgcc ggcagggtcg ccggctcatt catggtgccg tcgaagttcg
    13261 gcacccagtc cacggtaccc tggcccagtt cggtcagcag cacttccgag taacgcgaca
    13321 ggcgcgcctc ggtgtaacgc atggcggcga aggacttggg atcatccggc gcaccccagt
    13381 tgccctggcc gtcgaccaag gtgtagcgat agctgaaggg ctgggccatc agcaccatgg
    13441 cttcgtagca ggcgctgtcg ccgtgcgggt ggaacttacc gagcacgtcg ccgacggtac
    13501 gcgccgactt cttgtgcttg gagtcggcgt ccaggccaag ttcgctcatg gcgtagacga
    13561 tgcgccgctg cacgggcttg aggccgtcgc cgatatgcgg caacgcgcgg tccatgatca
    13621 cgtacatgga gtaattgagg taagcctgct cggtaaaatc ggcaagggaa cggcgttcaa
    13681 caccgtccag gctcaaatcg agggattcgc tcatgcgtgc ctcattgcaa agtgggttgt
    13741 tgcgaagttg tctggcgcag catcaaagtg ccgccgcgct gagtaaattc gagttgtttc
    13801 agggcactca tgcccagtaa tacgtcgtcg ccgcccatgc cgggtgcgat caaagcctct
    13861 acgtcggtca gcacgatatc gcccaactgc aggcgctcaa gccgagtgcg atgagccgtt
    13921 gcacggccat tggcggtgtt gatggtgatt gccgcgccgg cttgcaaacc caggcgctgc
    13981 gccacctcga tcggcaccgc gacctgcgtt gcgccggtat cgagaagaaa cgtcacctcc
    14041 tcgccattga tcttgccgcc ggccatatag tggccctggc gactgctggc caagtgcacc
    14101 tcaacatagc cgctgccatg cagcgactca ggcatctgat tggggttgcg ctgggcgtct
    14161 tcccagtcgc cgaagaactt ggtcgccagc aacagcgccg cgccccaggc cagcaccagc
    14221 atcacgcgcc cggcacgttt acccgcaggc tgctcgctca cggcttgctg ccccagccgc
    14281 ctttaggtcc ggcaaaacgc cagacaatcg gccgcgactc accgtcggct cgactgaaac
    14341 ggccgttatc gacgccgacc caggcaccat cctgatccac ccacaacgct tccgccatgc
    14401 cataggacgg cggatagagg cgctccggcg tcagcgcttc ggcagcaaac gaccagcact
    14461 tctcgacctg accattgctc aggctgcgcc gacagatgcg gtgcgcctga cgttcaaggg
    14521 taaagagctt ttcgttgtga aaggccaggc cggagaaatc ccgtggctgc tctttagcgc
    14581 ccagctgctc gggcgcaggc tccgtgcccg cttcgctgaa cagcacacag ccaccagtac
    14641 aacgccagat ggcttgttgc ttgtgcgcca ccagcagacc gcgacgcatg cgctcagcag
    14701 ccaggtacaa gcgctcgcca gccggatcga cagcaatacc ttcaaacagc gagttgtagt
    14761 gcaacaacat gccactggcg cgcgcctggc gtagcaggcc ctgaggcagt ttcagccaat
    14821 tgggctcacc tataaccggt aactgcagca cggccgcctt ggcctcgctg accaggaaac
    14881 ggttaccggc ggcgtcacaa ctgatgccct cgaaatccag ctcaccaccg cgcaccaggc
    14941 tggcagccca gctgcgcatg cgcagccccc agggcaagcg cgcatccggc gcaggcggcg
    15001 cgacaaaggt ctcggccgtg gcctgccact gcgactcatc ggattccagg cgatacagct
    15061 gctgatcatc gcgatcggaa accgcccaca gcgcgttatc gcaccaggct aggccagaca
    15121 ggttgccgcc actgacgccg ctaaccgctt gctcattgag cagcttcagc tcttccaata
    15181 ccgccgcact tgccagcaag ggtgagcaga gtagcaggca gacagccaac agccgctgca
    15241 tcagagcagc acctcggcca ggttgccctt ggactccagc caggatttac ggtctccggc
    15301 gcgtttcttg gccagcagca tgtccatcat ttcctgggtg ccggggtagt cgtccagggt
    15361 cagttgcacc agccgccggg tgttggggtc catggtggtt tcccgtagct gtggcgggtt
    15421 catctcaccc aggcccttaa agcgggtgac ttggggtttg ccacggcgct tctcggccac
    15481 cagacggtcg agaataccgt cgcgttctgc ctcatccagg gcgtagaaaa tctccttgcc
    15541 caggtcgatg cggtacagcg gcggcatggc gacataaacg tgacccgcat ccaccagcgg
    15601 acggaaatgc tgaacgaata gcgcgcaaag caaggtggcg atatgcaggc cgtcggagtc
    15661 ggcgtcggcg aggatgcaga tcttgccgta gcgcagctgc gagaggtcgc tggagcccgg
    15721 atcgagacca accgccaccg cgatgttgtg cacttcctgg ctggccagca cttcgctgcc
    15781 atcgacttcc caggtattca gaatcttccc gcgcagcggc aggatcgcct gaaattcttt
    15841 atcacgcgcc tgcttagccg aaccaccagc agaatcacct tccaccagaa acagttcaga
    15901 gcgcatcggg tcctgcccgg cgcagtcagc cagcttgccc ggcagtgccg ggccctgggt
    15961 gatgcgcttg cgttcaacct tcttgccggc cttaaggcgg cggttggcgt tgctgatcgc
    16021 cagttcggca agctgctgac ccagctccgg gtggccgtta agccacaggc taaaggcgtc
    16081 tttcactaca ccggaaacaa aggcagccgc ctcacgggac gacaggcgct ccttggtctg
    16141 cccggagaac tgcgcgtcct gcatcttcat cgagaggacg aaggcgatgc gctcccagat
    16201 atcttccggc gccagcttga caccacgcgg cagcaggctg cggaactcgc agaactcgcg
    16261 catggcatcc agcaggccct ggcgcaggcc gttgacgtgt gtgccgccct gagcagtagg
    16321 gatcaggttg acatagcttt cctgcaccgc gtcgccgcct tccggcagcc acaacagcgc
    16381 ccaatctact gcttctttat tacccgccag gctgccgcaa aatggctcat tgggtagccg
    16441 ttcaaattcg ctgaccgcat ctaccaggta ggagcgcagg ccgtcttcgt acatccactc
    16501 gactttctcg ccgctggctt tgtcctcgaa gctgaccgcg agccccgggc acagcacagc
    16561 cttggctttc agtacgtgct tgaggcggct aaccgagaat ttgaaggagt cgaaatactt
    16621 ggcgtccggc cagaagtgca cgctggtgcc ggtgttgcgc ttgcccacgg tgccgaccac
    16681 ggccaggtcg ctggccttgt agccgtcggc aaaactcatc tggtattcgt tgccgtcgcg
    16741 cttgaccgta acgatcacgc tggtcgacag cgcgttgacc accgagatac ccacgccgtg
    16801 caagccgccg gagaactggt agttcttgtt gctgaacttg ccgccggcgt gcagcttggt
    16861 gaggatcagc tcgacccctg gcacgccctc ttccgggtga atgtccactg gcatgccgcg
    16921 accgtcgtcg agcacttcca gggagttgtc ctcgtgcagg atcacctgaa tcgatttggc
    16981 atgcccggcc agtgcttcgt cgacgctgtt gtcgatgact tcctgggcca agtggttggg
    17041 ccggctggtg tcggtgtaca tgcccgggcg tttgcgcacc gggtcgaggc cggaaaggac
    17101 ttcgatggca tcggcgttgt aagagctaga accttactgg gccatggggt ctcttgttcg
    17161 tcaattgaga gcggaaaagt cgatatcctg ccacagatgg cgaggaatac cggcgaaagc
    17221 gaaaagcatc ggcaggcgtt cggcaaagcc ctgaaagcca tgatcgccac cggcctggat
    17281 acgcagcgcg caggcccggt aatagcgctg ggcatcacgg taatcgaggg tttcgtcggc
    17341 ggtctgcaac cacacctgat aacgcgccgg gtcttgcagc gccagcacat ccagttcggc
    17401 cagcgcagtt acatggtcgg ccgtcagctc ccaggtctca tcgctgtaat agttcttctg
    17461 cgggcccagg tagccgtcga aacgcagatg cgggcgcacg gctgggttga tcagcagcgc
    17521 cgacaaccca tggcgttcgg ccaagtgggt cgcatagtag ccgccaagcg agctgccaac
    17581 cagcacgggc cgaccaagct cggctatcag cgcttcgagc tgagcaatcg cctgccgtgg
    17641 atggtgatgc aggcccggca cccgtagctg tgcgtccaag cccagctgcg ccatggcgcg
    17701 ctgaagctgg ctggctttga gcgatgccgg cgagctgtta agcccgtgaa tatagagaat
    17761 ggatgtggtc atggggcggg aggctaaccg gttgcggcga ccggctgcaa ccggttagcc
    17821 gagcggctca atagcccttg atggtgtaat cgatttcgaa cttgataccg gtcacccgcg
    17881 acacgccggt ttccaggcga ccgtcgtcaa acagacgtag ccagcgatag cccggcgcgg
    17941 tcttatctac ctgaaattcc tcactgcccg gcgcgaactg cacgcaggtc gagggcgatg
    18001 ccagcaggcg gatcgggccg cgctgctgat cgagttcctg atggatatgc ccccagagca
    18061 ctgcacgcac ctggctgaaa cgctccagaa cggcaaacaa agcctccgga ttacgcagcc
    18121 cgatgggctc catccatttg caaccgatgg acaccgggtg gtgatgcagg caaatcaggt
    18181 gatgacgatc cggcacttcg ctgagtgcgc gctccagcaa ggtaagctgc tgctcactga
    18241 ggtaaccggg cacggcaccg gggatcgagg agtcaagcag ggttacgcgc cagttgccca
    18301 gatcgatcac tggctccaga agatcactgc caacgcaggc tgcctgcatg gctggcactt
    18361 catcatggtt accggggaac cagcgtgctg gcgcggggat ggcggcggtc atctcgcgga
    18421 aacgctggta ggactcagcg ctgccgtcct gcgagaggtc gccactggct aggatcaggt
    18481 caatctgcgg ctgctcctgc agcacacgct caatcacccg ctgcaagctg tcctgggtat
    18541 ccatgcccaa tagcttgcct gcctcgtcgg caaacaggtg actgtcggat aactgcacca
    18601 gcagtaccga gtgatcaaca gcagtggttt tagtcggcgc gcccggcaag accgtctcct
    18661 tgagcggaat gcatgaatta tgctggctag ccctgcgggc ggtaaacccg cgaaagggga
    18721 atagatcaca gaacgtccgc tgtaaatcca acgcacaccc taaggctagc gcgtattgga
    18781 ataatgcgcc tgcgactgat tatcgacaga acgtggaaaa acctaacgca cgggagccag
    18841 ttcgtggccg caggccaggc agtggctcag ccactcgccg aggaacaggt tgagttgggt
    18901 cttttcatcc ggctggtgca tcgcggcgtt ggggtaggga taaatgccgt gaaagcgccg
    18961 cgcactctgc gtgccgacca cttcggccat gcgcgcatca tgatagaccc gcacctccag
    19021 acgcggcacc ggcaaccagg acaggctgtg ctcctgacgc acctcgatgg tcgaggtata
    19081 ggggcaggtt tccagcacct ccagagccag cacaccgagt aggtgctcgc cctgactgag
    19141 ggccacacga cgaacctgct gcagcccctc cccgcgcatg tccggcagca gctgcagcag
    19201 gcgcacatag ttgagttcgc aagcggtctg cagctcgact aggtcgaccc gataacgctc
    19261 gcgcagcagg ttcatgacca cagcccccgg acttcggcac ggttcagcgc cagccactgc
    19321 aaggcgatga tgctggcggc attgtcgatc cgcccatctt taaccgcatc cagggcatct
    19381 tccagcggcc agacatgcac gcgaatgtcc tcgccctctt ccgccagacc atgcacaccg
    19441 ccggcacctt cgctgtcgca acggccgaca aacaaatgca cgcgctcatc cgagccgccg
    19501 ggtgatgggt agtactgggt aatcggccac agcgaggtca gcggcagatt ggcttcctcc
    19561 atcgcctcac gacaggcgac ttcctccggc tgctcatcct tgtcgatcaa tccggcgacc
    19621 agctccatca gccagggatt ggcgcttttc tccatcgcgc cgacacggaa ctgctcgatc
    19681 aacaccacgc aatcgcgttg cgggtcatag ggcagcacgc acaccgcatc gtgacgcacg
    19741 aacagttcgc gactcagctc cgggcccatg ccaccggcaa actggcgatg gcgcaaacgc
    19801 agccgatcga gcttgtagaa gccgctaaag cagctctcgc gctcaattat ctcaacatca
    19861 tctcgctgca tatccatccc ctattcaaca ggaacgggcc cgaaggcccg tcctggttac
    19921 tcccatactg ctacgcaaaa atcgcctagc agaagctcca tcacaccttg tggtagagct
    19981 gcgcaccctg cgccttaaac tccgctgcct tggcttgcat gcccgcctcg gcatccaggt
    20041 cgagtgcggc gatgcgttgc tcttcagcat agacgcgaac ttcctgggtg atcttcattg
    20101 agcagaactt cggcccgcac atggagcaga agtgtgccac cttcgccgag tccttgggca
    20161 gcgtttcgtc gtgataactg cgcgcggtgt cggggtcgag accgaggttg aactgatctt
    20221 cccaacggaa ctcgaagcgc gccttggaca atgcgttatc acgaatctgc gcgcccggat
    20281 ggcctttggc aaggtcggcg gcatgcgcgg cgatcttgta ggtgatgatg ccggtcttga
    20341 cgtcgtcctt gttcggcagg cccaggtgtt ccttgggcgt gacatagcag agcatggcgc
    20401 aaccgaacca gccgatcatc gccgcaccga tgccgctggt gatgtggtcg taacccggcg
    20461 cgatgtcagt ggtcagcggg ccgagggtgt agaacggcgc ctcgtcacag cattcgagct
    20521 gcttgtccat gttctccttg atcagctgca tcggcacgtg accggggcct tcgatcatgg
    20581 tttgcacgtc gtgcttccag gcgatcttgg tcagctcgcc gagggtttcc agctcgccga
    20641 actgcgcttc gtcgttggcg tcggcaatcg aaccaggacg caggccatcg cccagcgaga
    20701 agctgacgtc gtaggccttc atgatttcgc agatgtcttc gaagtgggtg tagaggaagt
    20761 tctctttatg gtgcgccagg caccacttgg ccataatcga accaccgcgc gaaacgatac
    20821 cggtgacgcg cttggcagtc atcggcacat aacgcagcaa tacgccggcg tggatggtga
    20881 aatagtccac gccctgctcg gcctgctcga tcagggtgtc gcggaacagt tcccaggtca
    20941 gttcctcggc tgcgccgcca actttttcca gggcctggta gatcggcacg gtgccgattg
    21001 gcactggcga gttgcggatg atccactcgc gggtttcgtg aatgtgcttg ccggtggaca
    21061 ggtccatcac cgtgtccgaa ccccagcgaa taccccaggt cagcttggcc acttcttctt
    21121 caatggacga acccagcgcg ctgttaccga tattgccgtt gatcttcacc aggaagttgc
    21181 ggccgatgat catcggctcc agctccacgt ggttgatgtt ggccggaata atcgcgcggc
    21241 cgcgggcaat ttcctcacgg acaaattcag cggtgatttc tttcggaatg ctggcgccga
    21301 agctatggcc tgggtgttgc tggtcgagca ggccggcggc gcggtgtt
//
LOCUS       DASRUM010000260        18963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_970159, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000260 DASRUM010000000
VERSION     DASRUM010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 18963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18963
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970159"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1640
                     /locus_tag="VFF22_17640"
     CDS             87..1640
                     /locus_tag="VFF22_17640"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZX18678.1"
                     /translation="MNAPVSPPSATRHCKVAIIGTGFSGLGMAIRLKQAGEHDFLLFE
                     KEAGVGGTWRVNNYPGCGCDVQSHLYSFSFEQNPDWTRMFAKQPEIQGYLEGCWSKYR
                     LQNHTQLNTEITRMAWDEQQELWQISDSTGNHYTAQFVVSGMGALSTPSIPALKGLGD
                     FTGKVFHSQQWDHSYDLTGKRIAVIGTGASSIQFVPQIQKQASQLDLYQRTAPWIMPK
                     PDRAISERERNRFKRFPLLQKLWRGAIYAMLESRVIGFAMLPKVMTLAQKVSKWHINK
                     QIKDPVLRAKVTPDYLMGCKRVLISNDYFPALTQPNVDLITDGIEEIRSGSIRTVDGK
                     EREIDAIIFGTGFTPSDPLPRGTVFGRGGVDLLDTWPEGPQAYKGTLTAGFPNLFFLM
                     GPNTGLGHNSMVYMIESQIHYVLGALKLLGEQELQSLEVKQAVQDKFNGKLQGSLGNT
                     VWNAGGCNSWYLHPVSGRNCTVWPGFTWRFRLLTRNFDPAAYHFSRKHAAHPAQSSAI
                     QLTAQEASA"
     gene            1637..2521
                     /locus_tag="VFF22_17645"
     CDS             1637..2521
                     /locus_tag="VFF22_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZX18679.1"
                     /translation="MKSFENKVAAITGAGSGIGRALAFGLARQGCQLALSDVNAEGLA
                     ETVAQARKLGVKVSETLVNVADREAVHAWADQVMADFGQINAIFNNAGVAQGGTVEGN
                     DYADYEWIMNINFWGVVHGTKAFLPHIKASGNGHIVNVSSVFGLFSQPGMSAYNATKF
                     AVRGFTESLRQELDMANCGVSASCVHPGGIKTNIAITARMNDSLSKVTGQDAEQARTQ
                     FNDQLLRTTPEKAAQVIINGVLGNKRRILIGPDAYALDGMQRLWPAFYQRLVTASMRL
                     AARFAPKGSSKKAQAAVE"
     gene            2677..3126
                     /locus_tag="VFF22_17650"
     CDS             2677..3126
                     /locus_tag="VFF22_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZX18680.1"
                     /translation="MTTQDPCAELLLDNQLCFALYSTSLLLTKVYKPLLQELGLTYPQ
                     YLAMMVLWEGDGITVGEISSRLLTDPGSVTPLLKRLEVEGLLKRTRSSVDERVVELFL
                     TEKGRSLREQAKRIPGCILTATQQSAAELGALKAELVTLRSSLQQAV"
     gene            3642..4070
                     /locus_tag="VFF22_17655"
     CDS             3642..4070
                     /locus_tag="VFF22_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HZX18681.1"
                     /translation="MSIQTIAYRAYEEATGGRDGRAISSDGILDVALTTPKELGGAGG
                     AGTNPEQLFAAGYSACFIGAMKFVAGRDKLAMPADASIEGVVGIGAIPNGFGIEVELR
                     ISLPGMDREQAQTLIERAHIVCPYSNATRGNIDVTLTLLD"
     gene            4124..5140
                     /locus_tag="VFF22_17660"
     CDS             4124..5140
                     /locus_tag="VFF22_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX18682.1"
                     /translation="MNIKQGLSIAVLALAVNSAFAAGSPGVERTTQGFLNALAAGGGK
                     PLEQLSPKEARAVLVGAQAGVKVDVSGIKVVDKTIEVDGQSIQLKVVRPSDAKGTLPV
                     FMFFHGGGWVLGDYPTHERLIHDLVVGSGSAAVYVDYTPSPEAKYPTAINQAYAATQW
                     VAQNGAQIGVDGKRLAVAGNSVGGNMAAVVSLMAKDKGTPAIKFQLLMWPVTDANFNN
                     ASYNQFADGHFLSKSLMQWFWDNYTTDPKQRAEIYASPLQASVEQLKDLPPALVQIAE
                     FDVLRDEGEAYARKLDAAGVNVTSVRYNGMIHDFGLLNVISQIPGTQSAMQQAAAALK
                     ANLQ"
     gene            complement(5526..5936)
                     /locus_tag="VFF22_17665"
                     /pseudo
     CDS             complement(5526..5936)
                     /locus_tag="VFF22_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914005.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            complement(5980..6717)
                     /locus_tag="VFF22_17670"
     CDS             complement(5980..6717)
                     /locus_tag="VFF22_17670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX18683.1"
                     /translation="MLNIQDKVILITGASSGIGEACARLLAEKGARLLLGARRTERLE
                     QLVGEIREAGGNADYRRLDVTSQADTQAFIDAALAQYGRVDVLINNAGVMPLSPLEAL
                     KVDEWNRMIDVNIRGVLHGIAAALPIMQRQRSGQFVNIASIGAYSVVPTGAVYCATKY
                     AVRAISEGLRQEVGGDIRVTLVSPGVVESELAESISDASSREAMKAFRKVAITPDAIA
                     RAIAYAVEQPADIDVSELIVRPTASPY"
     gene            7018..7944
                     /locus_tag="VFF22_17675"
     CDS             7018..7944
                     /locus_tag="VFF22_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZX18684.1"
                     /translation="MTSSISPSALDDNLAARQAELAALIQRQCPGDGLHSSAIAGLDL
                     ACATQPSLPVPTLYRPCLCIIAQGRKNVRLGDEQYTYDPLNYLVASVTLPVTGQVIGA
                     TPEQPYLSLRLEIDPALITSLIADIGPIGVPNPGPRRALYLDRLDTQLLDAVLRLLRL
                     QETPRDIAMLAPLALREIFYRLLHSPQGHRLHEIAIADSQGQRINRAIEWLNLHFDQP
                     LRIEDLAREVNLSTSTLHHRFKAVTALSPLQYQKQLRLQEARRLMLCEGLEAASASYR
                     VGYESPSQFSREYSRLFGAPPLRDLARLRSSA"
     gene            8293..8505
                     /locus_tag="VFF22_17680"
     CDS             8293..8505
                     /locus_tag="VFF22_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016488765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZX18685.1"
                     /translation="MANRETGTVKWFNDEKGFGFITPTNGGDDLFVHFKAIQSDGFKS
                     LKEGQTVTYVAARGQKGMQAEEVQVA"
     gene            complement(8581..9729)
                     /locus_tag="VFF22_17685"
     CDS             complement(8581..9729)
                     /locus_tag="VFF22_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZX18686.1"
                     /translation="MSVFQEYFDESHQLVRDSVRRFVEREILPHINDWEEAEEFPREL
                     YLKAGAAGILGIGYPEAFGGSHEGDVFAKVAASEELMRSGSGGLVAGLGSLDIGLPPV
                     VKWAKPELRERIVPQVLAGEKIMALAVTEPSGGSDVANLKTRAVRDGDFYRVSGSKTF
                     ITSGVRADYYTVAVRTGGDGFGGVSLLLVEKGTPGFTVGRKLKKMGWWASDRAELFFD
                     DCRVPVGNLIGAENMGFACIMANFQSERLSLAIMANMTAQLALEEALKWAKEREAFGK
                     PIGKFQVLKHRLAEMATQLEVSRDFTYRQAAKMAAGKSVIKEISMAKNFATDIADRLT
                     YDAVQIMGGMGYMRESLVERLYRDNRILSIGGGSREIMNEIISKQMGL"
     gene            complement(9838..10437)
                     /gene="recR"
                     /locus_tag="VFF22_17690"
     CDS             complement(9838..10437)
                     /gene="recR"
                     /locus_tag="VFF22_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003254304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HZX18687.1"
                     /translation="MSFSPLIRHLIDSLRILPGVGQKTAQRMALQVLERDRSGALRLA
                     QALTQAMEGVGHCKQCRSLSEDEVCQLCLDPRRDDSLLCVVEGPMDVYAVEHTGFRGR
                     YFVLKGHLSPLDGLGPEAIGIPELLARIAVGSFTEVILATNPTVEGEATAHYIAQLLA
                     GKGLIASRIAHGMPLGGELELVDGGTLAHSIAGRRPIQL"
     gene            complement(10581..10907)
                     /locus_tag="VFF22_17695"
     CDS             complement(10581..10907)
                     /locus_tag="VFF22_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HZX18688.1"
                     /translation="MMKGGMAGLMKQAQQMQEKMQKMQEELANAEVTGQSGAGLVSVV
                     MTGRHDVKRVSLDDSLMQEDKEILEDLIAAAVNDAVRKVEQNSQDKMSGMTAGMQLPP
                     GFKMPF"
     gene            complement(10926..13019)
                     /gene="dnaX"
                     /locus_tag="VFF22_17700"
     CDS             complement(10926..13019)
                     /gene="dnaX"
                     /locus_tag="VFF22_17700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HZX18689.1"
                     /translation="MSYQVLARKWRPRSFNEMVGQTHVLKALINALDSQRLHHAYLFT
                     GTRGVGKTTIARIIAKCLNCETGISSTPCGICSICKEIDEGRFIDLIEVDAASRTKVE
                     DTRELLDNVQYSPSRGRFKVYLIDEVHMLSTSSFNALLKTLEEPPPHVKFLLATTDPQ
                     KLPVTVLSRCLQFSLKNMPPERVVDHLTHVLAAENIPFENDALWLLGRAADGSMRDAM
                     SLTDQAIAFGEGKVLAADVRAMLGTLDHGQVYGVLHALLEGDARGLIEAVRHLAEQGP
                     DWNGVLSEMLNVLHRVAIAQALPEAVDNGQGDRERVLELARVLPSEDVQFYYQMGLIG
                     RRDLPLSPDPRSGFEMVLLRMLAFRPADTHDAPRVALKPLGISQATADPATNPVAGAA
                     IVAPVSAPVATSPAPQPAPVIEEVPVAPVAVPEIPTAVMPQMQADPEFVEVVAVQPSP
                     VEIAPAPAPAPAPAPVVAAVVDLPWEEPVRAPLAEVSEPVVAIAPEVITEAAPPAVLE
                     SQPAEPVPLVDADDDEPPLGDYDYVEMDAESFDYDFDAVERAASSEPEVLPAAQPATG
                     LAADWLELFPKLGLSGMTGSIGANCTLISVEGDNWLLHLDPAHSALFNPTQQRRLNDA
                     LNQFHGRELKVLIELCKPEQETPAQAAASKRANRQREAEASIHQDPVIQQMIQQFAAV
                     IRADSIEPLDTPVNP"
     gene            13482..13703
                     /locus_tag="VFF22_17705"
     CDS             13482..13703
                     /locus_tag="VFF22_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008051046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18690.1"
                     /translation="MNTQHQIQDAIRTLSSAFAPFDCRILAARKGSFSFTIVNDAGIA
                     KHSQRLYPGQYSGAHLEQVIERARQSLTA"
     gene            13774..14061
                     /locus_tag="VFF22_17710"
     CDS             13774..14061
                     /locus_tag="VFF22_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3509 domain-containing protein"
                     /protein_id="HZX18691.1"
                     /translation="MDNINRELVEQLFAPLRATFSPPRPDGGIVLSLLDASDATAYSR
                     VLSAAQRTDSQAFAQSLEDIRLELAMRSGSIPADMRKALKEQDSVLSYHTS"
     gene            complement(14196..15140)
                     /locus_tag="VFF22_17715"
     CDS             complement(14196..15140)
                     /locus_tag="VFF22_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003196594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HZX18692.1"
                     /translation="MESLNLDGWLPIRVWQEAGRWQVDWCWFGDMRLSKPFFGDAVEE
                     ALRLPFNQAFRRQTPLSALGQWQQQRPGLAPAVFIFHASRCGSTLISQMLAQLDEHIV
                     ISEPPPLDTLLRSDMASAERCMALKGLMSAYGQRRRGLEQRLVIKLDAWNIGELPLLL
                     TCFPTTPWLFVYRDPLEIAVSHLRRPGMHMVPGMIGASVLDDAIPFSSSEDFISRRLG
                     KLLQKGLERCQEFSGVVVNYNELPDAMAGRLATFFGLNVDQCKLVFAAVGQHSKQPVQ
                     AFSCDSENKRQEASQLLKDHVMQTAFGAYAALESLRGG"
     gene            complement(15128..15913)
                     /locus_tag="VFF22_17720"
     CDS             complement(15128..15913)
                     /locus_tag="VFF22_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011331878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HZX18693.1"
                     /translation="MTRPAFSRLPLPVDLPLLLQALAAIEHDRWQGHFNSAYYSGDWS
                     GVALITPADALTELSPGRGQPMHRAPWSADARWCQALCDWPLHIVSARLLRLGPGGRI
                     HEHRDYDLGGPDADLRLHIPLLSPPGVDFWLDEQRIPMTAGECWFLDLSRPHRVDNRG
                     TQVRIHLVLDCRQEPWLLQQITDGLACTPAARHAQSDFVRFQRCVETSPQLTQTLQGL
                     QDVEAFIECALRLAAEQGLFLTRQALQAAMRNGRRQWSEQWKV"
     gene            complement(15926..17437)
                     /locus_tag="VFF22_17725"
     CDS             complement(15926..17437)
                     /locus_tag="VFF22_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003196589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZX18694.1"
                     /translation="MNKSQKLFGMSLLALVVSGCAVTSEPIERSVSEERARTDLQNMY
                     KDQEPLSGPLTLHQAMARAVKYNLEGRLKIMEEALAKRQLDLASFDMLPRMALDAGYV
                     GRNNVSASSSESVETGTQSLEPSTSQDRDREVADLTMVWNVLDFGVSYISAKQAGDQR
                     LIVQERRRKVINTIVQDVRSAYWRAMAAERLLKQIDSLMARVETARDNSQSMSEQRIG
                     DPVQALGYQRSLIQATRQLEEQRRALSLAKTELATLVNLPLGTQLTLATPDEYVIPEL
                     KVDLASLEQEALTSRPELREQDYQTRITAAETRKAMLRLLPGLEFSAGGHYDSNSFLV
                     EQGWIDYGVKVTWNLFNVISAPAAIDVAKAGEEVAAARRQAMSIAVLAQLYVANANYR
                     EALRQFKTNQQLSDIDGQIVGQLRNRYQAAGLGELELIQGELNTLQADLRRDLSYADL
                     RNAYGQIFASAGLDPLPDEVQSTEVQSIATALANREAAWAEGDIGVPMAALPQ"
     gene            complement(17478..>18963)
                     /locus_tag="VFF22_17730"
     CDS             complement(17478..>18963)
                     /locus_tag="VFF22_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003186897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZX18695.1"
                     /translation="AIDTQVPTATIVVADTSLIIGETSLVTITFSEAVSGFDNSDLTV
                     SNGTLSSMSSTDGGVTWTATFSPSSNISDTSNMITLDNTGLINIAGNAGIGNTDSNNY
                     VVDTIRPGASITLADTRLGIGQTTTVTIGFTEAVTGFDLSDLSVANGVLSNLTSSDGG
                     LTWTATLTPSAGVNDTTNLILLDSSNVQDIAGNAGAGIAISANYALDATRPTATIVVA
                     DPNLSVGETTQVTITFGEAVSDFDLSDLSVTNGELSNLSSSDGGLTWTATFTPTADLT
                     DPSNFIALDTSNVSDLASNAGASVAVSNNYVIDTVAPNEVKPPPEFLTSDPVTVVPPA
                     EVPLQPIVFTPPTGDLGSPLGFPPLFEQRDVGGGIRPIGDIFINRGALAPSFIAQAFS
                     SDAAGNGAAQGFLGFGGGDGGVFGGSTISGLFSQDFASESDSMDVFGNQSMQGGDGAQ
                     GVPGLLFGAPTLGQQLQHLKDTEQRQVNNLAAALQQVGISQVQA"
BASE COUNT         4065 a         5998 c         5230 g         3670 t
ORIGIN      
        1 agaatggcac ttgctggcga gtatacattt gtctaccttg gtatacagct gtatacgcca
       61 gcaataaaca taacaggagc ttggccatga atgcacccgt ctcaccccct tctgccaccc
      121 gccactgcaa ggtcgccatt atcggtacgg gcttctccgg cctgggcatg gccatccgcc
      181 tgaagcaggc cggcgaacat gacttcctgc tgttcgaaaa agaagccggc gttggcggca
      241 cctggcgggt caacaactac ccgggttgcg gctgtgacgt gcaatcgcac ctctactcct
      301 tctccttcga gcagaacccc gactggacgc gcatgttcgc caaacagccg gagatccagg
      361 gctacctgga aggttgctgg agcaagtacc gtctgcagaa ccatacccag ctgaacaccg
      421 agatcacccg catggcctgg gatgaacagc aggagctgtg gcagatcagt gacagtaccg
      481 gcaatcatta cactgcgcag ttcgtggtgt ccggcatggg tgcgctgtcc accccatcga
      541 ttccggcgct caaggggctg ggcgatttca ccggcaaggt cttccactca cagcagtggg
      601 atcacagcta tgacctcacc ggcaaacgca tcgcggtaat cggcaccggc gcctcctcaa
      661 tccagttcgt gccgcagatc cagaagcagg ccagccaact cgacctgtac cagcgcactg
      721 cgccgtggat catgcccaag cctgaccgtg ccatcagcga acgcgaacgc aaccgcttca
      781 agcgcttccc actgctgcaa aaactctggc gtggcgccat ctacgccatg cttgaaagcc
      841 gggtaatcgg ctttgccatg ctgcccaagg tgatgaccct cgcccagaaa gtcagcaagt
      901 ggcacatcaa caagcagatc aaggacccgg tcctgcgcgc caaggtcacc ccggattacc
      961 tgatgggctg taaacgcgta ctgatctcca acgattattt cccggcgttg acccagccca
     1021 acgtcgacct gatcactgac ggtatcgagg aaatccgcag cggcagcatc cgcaccgtgg
     1081 acggtaaaga gcgcgagatc gacgcgatca tcttcggtac aggtttcacc cccagtgacc
     1141 ctctcccgcg cggcacagtg tttggccgtg gcggcgtaga cctactcgac acctggccgg
     1201 aaggcccgca ggcctacaaa ggcaccctca ctgccggctt ccccaacctg ttcttcctga
     1261 tggggccgaa caccggcctg ggccacaact cgatggtcta catgatcgag tcgcagatcc
     1321 attacgtgct cggcgcgctc aaactgcttg gtgagcaaga attgcagagc ctagaggtca
     1381 agcaggcggt gcaggacaag ttcaacggca agctgcaagg cagcctgggc aacaccgtat
     1441 ggaatgcagg cggctgcaat agctggtacc tgcacccagt cagcggccgc aactgcaccg
     1501 tgtggcccgg ttttacctgg cgcttccgtt tgctgacgcg caacttcgac ccggctgcct
     1561 atcatttcag ccgcaagcac gcggcacacc cggcgcagag cagcgctatc cagttgactg
     1621 cccaggaggc cagcgcatga agtcgtttga aaacaaggta gccgccatca ccggcgccgg
     1681 ttccggcatc ggtcgcgccc tggccttcgg tctggcccgc cagggttgtc agttggcgct
     1741 ctcggatgtg aatgccgaag ggctggccga gacggtcgct caggcgcgta aactcggagt
     1801 gaaggtcagc gaaaccctgg ttaacgtcgc cgaccgcgag gctgtgcatg cctgggcgga
     1861 tcaggtgatg gccgacttcg gccagatcaa cgcgatcttc aacaacgccg gcgtggccca
     1921 gggcggcacc gtcgaaggca atgactacgc cgactacgag tggatcatga acatcaattt
     1981 ctggggcgta gtccacggta ccaaggcttt cttgccgcat atcaaggcgt cgggtaatgg
     2041 ccatatcgtc aacgtatcca gcgtcttcgg cctgttctcc cagccaggta tgagcgccta
     2101 taacgccacc aagttcgccg tacgcggctt taccgagtcg ctgcgtcagg agctggacat
     2161 ggccaattgc ggcgtgtcgg ccagctgcgt gcacccaggc gggattaaga ccaatatcgc
     2221 cataaccgct cgaatgaatg acagcctgag caaggtcacc ggccaggatg ccgagcaggc
     2281 ccgtacgcag ttcaacgacc agttgctgcg caccaccccg gagaaagccg cccaagtgat
     2341 catcaacggt gtgctgggca acaagcggcg cattctgatc ggcccggacg cctatgcctt
     2401 ggatggcatg cagcgcttgt ggccggcgtt ctatcagcgc ctggtaaccg cctccatgcg
     2461 cctggctgcg cgtttcgcgc cgaaaggctc aagcaagaaa gcccaggctg ccgtggagta
     2521 atcggcaacc gtccaagccc cagcgccgca ggtgtctggg gctttttcat gtttggtgtt
     2581 tgcctgcgag gaaggattac cgcgtgcatt caattggcat aacagtagtt agcacgataa
     2641 attgtcggtc actgtcctgt gccgctggag aacgccatga ccacgcaaga cccttgcgcc
     2701 gaactgctgc tcgataacca gctgtgcttt gccctgtatt cgacttcgct gctgttgacc
     2761 aaggtctaca agccgctgct gcaggagctg ggcctgacct acccgcaata cctggcgatg
     2821 atggtgctct gggaaggcga tggcatcacc gtgggcgaga tcagcagccg cctgctcacc
     2881 gacccaggtt ccgtcacccc gctgctcaaa cgcctggaag tcgaaggtct gctcaagcgc
     2941 acccgcagca gtgttgacga gcgtgtggtc gagctgttcc tcaccgagaa aggccgcagc
     3001 ctgcgcgaac aagccaagcg catccccggt tgcatcctca ccgccactca gcaatccgct
     3061 gcagaactcg gcgccctcaa agctgaactg gtcaccctgc gcagcagcct gcagcaagcc
     3121 gtataagtca cctgggcgac agatatgtcg cccgccccaa cggtctggtt aaacagatcg
     3181 cgtcacgtat accctgtcat ttccccgctc cgtacaataa tttcgccctg ccgccacgct
     3241 caaaaaatcg cgcaatcgcc cgccaatcct cgcttatgcc cgaaccaagc agcacttatc
     3301 gctaaatgat caaaaaaata tcttgcgcgc taaattaatg cgctctagag ttcaccccat
     3361 ggaactgatt agcgcacaaa acattagcgc gaaaagacca cgaaaacctg cagttgtctt
     3421 cacctaaccc aacaacctga ctgtcgtgga gaaccgttat gaaaaccctg atcgctttca
     3481 ccgccctgct cgccctttcc gccccagttc tggctgaaga cagccagaca gacggcaaag
     3541 gcctttaccc gaactggctg atcacccacg aaggttgatc accgcctgac accgcaacgc
     3601 cttaaacaaa ccaacccaaa cagttccaga ggattaccgc catgtcgatt caaaccattg
     3661 cttaccgtgc ctatgaagaa gccaccggcg gccgtgacgg ccgtgccatc tcctccgacg
     3721 gcatactcga cgtcgccctg accaccccga aagagctcgg cggtgcgggc ggtgcgggca
     3781 ccaaccctga gcagctgttc gctgcgggct actcagcctg ctttatcggc gcgatgaagt
     3841 tcgtcgcggg gcgcgacaag ctggcgatgc ctgcggacgc ttcgatcgaa ggtgtggtgg
     3901 gtatcggcgc aatccccaac ggtttcggca tcgaagtcga attgcgtatc agcctgccag
     3961 gtatggaccg tgagcaagcg cagaccctga ttgagcgtgc gcacatcgtc tgcccttact
     4021 ccaatgccac ccgcggcaat atcgacgtca ccctgacgct gctcgactaa ctcacgactc
     4081 atccggccgc tgccagtcag cggcccgccc caggaggctg cacatgaaca tcaaacaagg
     4141 tttgtcgatc gccgtactcg cccttgccgt caacagcgcg tttgccgctg gtagccccgg
     4201 ggtggagcgt actactcaag gtttcctcaa tgccctcgcc gctggcggtg gcaagcccct
     4261 cgaacagctg agccctaaag aggcgcgcgc tgtattggtt ggcgcacagg ctggggtcaa
     4321 ggtcgatgta tcgggcatca aggtggtcga caagaccatc gaggtcgatg gtcagtcgat
     4381 ccagctcaaa gtcgtgcgcc catccgatgc caaaggcacg ctgccggtct ttatgttctt
     4441 ccacggtggc ggctgggtgc tgggcgacta cccgacccac gaacgcctga tccatgacct
     4501 ggtggtgggc tccggctcgg cagcggtgta tgtcgattac accccgtcac ccgaagccaa
     4561 gtacccgact gcaatcaacc aggcctatgc cgcaacccaa tgggtggcgc agaacggcgc
     4621 gcagattggt gtcgacggca agcgcctggc ggtagctggt aacagcgtgg gcggcaatat
     4681 ggccgcggtg gtcagcctga tggccaagga caaaggcacc ccggcgatca agttccagct
     4741 gctgatgtgg ccggtcaccg acgccaattt caacaacgcc tcctataatc agtttgccga
     4801 tggccacttc ctcagcaaga gcctgatgca gtggttctgg gataactaca ccaccgatcc
     4861 aaaacagcgc gccgaaatct acgcctcgcc gctgcaggca tcggttgagc aactaaaaga
     4921 cttgccgccg gccctggtgc aaatcgccga gttcgacgtg ctgcgcgacg aaggcgaagc
     4981 ctatgcacgc aagctggacg cggctggggt caacgtcacc tcagtgcgct acaacggcat
     5041 gatccatgac ttcggtttgc ttaacgtgat cagtcagatc cctggtacgc aaagcgccat
     5101 gcagcaagcg gccgctgcgc tgaaagccaa cctccagtag tccaacccga aaaacgcctt
     5161 gtcgccagtg caccaaggcg tttttgtgcc cggaatcaag cgaagcgaca cccaagaacg
     5221 ggcgctagca ctccacgctg gcgagtagac agtggcggat ggcctgatcg aatacctgtt
     5281 gcaaccgggg cgccacaaaa aatccatgca gggctgcatc cgactgccag cagccctgca
     5341 attgccacga cgcaggatct cccggcacac gcgttaccgc aaagcccaga cacccgacca
     5401 tctgctgcat gggctcctgc aatgtatgca ggcactgacc caattgttca gaacgcccac
     5461 gaacggcgcg caccactgtc gtgtgggtaa ccatcttcgt tactccctct atcagtgaaa
     5521 gtacgttacg gctgcgcagc caatacctcg cgcagcgcct gagccgcatc cagagcacgg
     5581 gggaaacctg cagtgaccgt tgtcaggtaa atcatttact tgagttcggc tggcaacacc
     5641 ccggcttgca atgcgtaacc ggcatggatt ttcatctggg gtaaattgcc ctgagcggca
     5701 aatacggcca ccgtagccag ttgccgcgta cgcgcatcca gctcactgcg cccccagaca
     5761 tcccccaacg catagtcggt agtggcgctg gctagaaaag gaaagtcctt atgcagggcc
     5821 tgcaacaccg gctgccctgc tccgccactc agctcggcta ttactgcctc acctttactt
     5881 ctgcggcggt cctcggttgc aagggcgtca agacaagcta tcgcaagaaa caccatcact
     5941 aatggcgtca gtaaccattt actgttcatg cgcacgtcct cagtaagggc tggcagtcgg
     6001 ccgcacgatc aactcgctga catcgatatc cgccggctgc tcgaccgcat aggcgatggc
     6061 acgggcaatc gcatccggag taatcgcgac cttgcggaac gccttcatcg cctctcggct
     6121 cgacgcatcg gaaatactct cggccagctc cgactcgacc acgcccggcg ataccaaggt
     6181 cacgcgaata tcgccaccca cttcctgacg caggccttcg gagatggccc gcaccgcgta
     6241 cttggtcgcg caatacaccg cccccgtcgg caccacgctg tacgccccga tggaggcgat
     6301 attgacgaac tgaccgctgc gctgacgctg catgatcggc agcgcggcgg cgatgccatg
     6361 cagcacgcca cggatattca cgtcgatcat gcggttccac tcatcgacct tgagcgcctc
     6421 gagtggcgaa agcggcatca ccccggcgtt attgatcagc acatcaacgc ggccgtactg
     6481 cgccagggcg gcatcgataa atgcctgagt gtcggcctgg ctggtgacat ccaggcggcg
     6541 gtaatccgcg ttgccgcccg cctcacggat ctcgccgacc aactgttcta ggcgctcggt
     6601 acgtcgcgcg ccgagcaaca gccgcgcgcc cttctcggcc agcaaacgcg cacaggcctc
     6661 gccgataccg ctgctggctc cggtgatcag aatgactttg tcttggatat tcaacatggc
     6721 cgaatcctct gctggtaagc ggcaaggatt gccgcgacag caacagattc cgtcagatgg
     6781 cgtgctcagg gttagacgat ttctggcgaa ttattgccta aaaatgcagc gctcaaaatt
     6841 cagcaacact actctgcgaa acctacgctc aattcagact tgtgctgatc gcctgtagaa
     6901 cctgggttat tgcctattgc tggtgaatta ttgcctaatc ctgcaacggc ttatgtacac
     6961 cgctgacttg cctgaaaaac ctggctagtc tgcaagccag caacaggagc cacgcccatg
     7021 acatcttcta tcagcccctc agccctcgac gacaacctgg cagcgcgcca ggccgaactg
     7081 gccgcactga tccagcgcca atgtccaggg gatggtctgc atagcagcgc cattgccggc
     7141 ctcgacctgg cctgcgcgac ccagcccagc ctgcccgtac cgacgctgta ccggccatgc
     7201 ctgtgcatca ttgcccaggg ccgcaagaat gtgcggctcg gcgatgagca atacacctac
     7261 gatccgctga actatctggt ggcctccgtc accctgccgg tgaccggcca ggtgatcggc
     7321 gcaacccccg agcagcctta tctaagcctg cgcctggaga ttgatccggc gctgatcact
     7381 agcctgatcg ccgacatcgg ccccattggc gtgcctaatc ctgggccacg tcgggcgctg
     7441 tatctggatc gcttggatac tcaattgctc gacgcggtat tgcgtctact acggctgcag
     7501 gaaaccccgc gcgatatcgc catgcttgcg ccgctggccc tgcgcgagat tttctaccga
     7561 ctgctgcaca gcccgcaggg ccatcgcctg cacgaaatcg ccatcgccga cagccagggc
     7621 cagcgcatca accgcgcaat cgaatggctg aacctgcatt tcgaccaacc cctgcgcatc
     7681 gaggacctgg cccgcgaggt caatctcagc acctcgaccc tgcaccaccg cttcaaggcc
     7741 gtcaccgccc tcagccccct gcagtaccaa aagcagttac gcctgcagga agcgcggcgc
     7801 ctgatgctct gcgaagggct ggaagcggct tcggccagtt accgcgtcgg ctatgaaagc
     7861 ccctcgcagt tcagccgcga atacagccgc ctgttcggcg cgccaccgct acgcgatctg
     7921 gcccgcttac gcagctcggc ctagcacagc tagaaatcga ccgctgacaa cagactggaa
     7981 aacaaacgcc ccggcgcttt tcaagagcat cggggcgttt tatttgggtc aaattaccgt
     8041 tacagcaggc aaatctggcc agaaaccatc accggcaaca gcatctttca gatgtttaac
     8101 cgccctgaaa cccaggcaaa tagggctctt gcgggttttc aacagagaaa aatatgaata
     8161 attaacgtta aacccaacac ttcccgtaaa aacggcctat tatggctgag ctgacttgta
     8221 gttatcagca aaaccgcata ggtgctatga gcactctggc accgcacttc gtatcgattt
     8281 aagagacaca ccatggcaaa tcgcgaaact ggcaccgtca aatggttcaa cgatgagaaa
     8341 ggcttcggct tcattacccc gactaacggc ggcgatgatc tcttcgttca cttcaaagct
     8401 atccaaagcg acggtttcaa aagcctgaaa gaaggccaga ctgttaccta cgtggccgct
     8461 cgcggtcaga aaggtatgca agctgaagag gtacaggtgg cctaagcccc tgtcctgctt
     8521 taaaacaaac cccgcggccg cggggtttgt tttatctgcc gtttgtaaaa ccttgccgct
     8581 ctataaaccc atctgcttgc tgatgatctc attcatgatt tcacgcgagc caccgccaat
     8641 cgacagaatg cggttatcgc ggtatagccg ctccactagg ctttcgcgca tataacccat
     8701 gccacccatg atctgcaccg catcgtaggt cagtcgatca gcaatatcgg tggcgaagtt
     8761 cttggccatg gatatttcct tgatcacgct tttacccgcc gccatcttcg ccgcttgccg
     8821 ataggtaaaa tcgcgcgaca cctcaagctg cgtagccatc tccgccaaac gatgcttgag
     8881 cacctggaac ttgccgatag gcttgccgaa cgcttcgcgc tccttggccc atttcagtgc
     8941 ctcctctagc gccagctgcg ccgtcatatt ggccatgatc gccagcgata agcgctcgct
     9001 ctggaagttc gccatgatgc aggcaaagcc catgttctcg gcaccgatca ggttgcctac
     9061 cggcaccctg caatcatcga agaacagctc cgccctgtcc gacgcccacc agcccatctt
     9121 cttcagtttg cgtcccacgg tgaagcctgg cgtgcccttc tccaccagca gcaggctgac
     9181 gccgccaaaa ccatcgccgc cagtacgcac ggcaacggtg taataatccg cgcgcacacc
     9241 actggtaata aaggttttgc taccgctgac gcgatagaaa tcaccatctc gcaccgcgcg
     9301 ggttttcagg ttggccacat ccgaaccacc ggacggctcg gtcaccgcca acgccatgat
     9361 tttttcaccg gccagcactt gcggcacgat gcgctcgcgc agctccggtt tggcccactt
     9421 caccaccggc ggcaaaccga tatccagcga accgaggcct gctaccagac caccagagcc
     9481 tgagcgcatc aactcctcgc tggccgcgac cttggcaaat acatcaccct catggctgcc
     9541 gccaaaagct tccgggtaac caataccgag aatccccgcc gccccggcct tgaggtagag
     9601 ctcgcgagga aactcctcgg cttcttccca atcattgatg tgcggcagaa tttcgcgctc
     9661 gacaaagcgc ctgactgaat cacgcaccag ctggtgggat tcatcgaaat attcctggaa
     9721 gacagacatg aggtattccc actgaggttt cagggaaatt accaagcgct tgcttggttt
     9781 acaagaagag gattacgcca tgcacaaggg caatcaagcc gcctgccagc caggaaccta
     9841 cagctggata ggccggcgcc cggcaatcga atgcgccagg gtgcccccgt caaccaactc
     9901 cagctcgccg cctagcggca tgccgtgggc aatgcgtgag gcaatcaggc ctttgcccgc
     9961 cagcaactga gcgatgtaat gcgcggtggc ctcgccctct acagtcggat tggtggccag
    10021 aatcacctcg gtgaaactgc cgacagcaat ccgcgccagt agctcgggaa taccaatcgc
    10081 ctctgggccg agaccgtcga gcggcgacaa atgccccttg agcacaaagt agcgcccgcg
    10141 aaacccggtg tgctcgaccg catacacatc catcggcccc tcaaccacgc acagcaagct
    10201 gtcatcgcgc cgcggatcca gacacagctg acacacctcg tcctcgctca ggctgcggca
    10261 ctgcttgcag tggcccaccc cttccatcgc ctgcgtcagc gcttgtgcca gacgcaaggc
    10321 gccggagcga tcacgctcaa gcacctgcaa agccatacgc tgggcagtct tctgcccaac
    10381 gccgggaaga atgcgcagtg aatcgatcag gtggcggatc agcggactga agctcatggg
    10441 aaatactcga tgactactaa cgcttaccag caaaacccta cagggctatt gagccatgac
    10501 agatcaaggc tcaggcaggt gtgggctgag gatacccaca cctgtttgca gcgcagccat
    10561 gtgcgctgac ccgatgcttt ttagaacggc attttgaagc caggcggcaa ctgcatgccg
    10621 gcagtcatac cggacatctt gtcctggctg ttctgctcaa ccttgcgcac ggcatcgttt
    10681 accgcagcgg cgatcaggtc ttcgaggatt tccttgtctt cctgcatcag gctgtcatcc
    10741 aggctgacac gcttgacgtc gtgacgccca gtcatgacca cgctgaccag cccagcaccg
    10801 gattgaccgg ttacctcggc gttcgccagc tcttcctgca tcttctgcat tttttcctgc
    10861 atctgctggg cctgcttcat caggccggcc atgccacctt tcatcatggt gtaagtcctc
    10921 ggtaattagg ggttaacggg agtgtccagg ggttcaatgc tgtccgcgcg gataaccgcg
    10981 gcgaactgct gaatcatttg ttgaatgacc gggtcctgat ggatcgaggc ttcggcctca
    11041 cgctggcgat tggctcgctt actcgccgcc gcctgcgccg gcgtctcctg ctcaggcttg
    11101 cacaactcga tcaatacctt gagctcgcgc ccgtgaaact gattgagcgc atcattcagg
    11161 cgccgctgct gagttgggtt gaacagcgcc gaatgagcag gatccaggtg cagtagccag
    11221 ttatcgccct caacactgat cagggtgcag ttggcgccga tactgccggt catcccggac
    11281 aagccgagct tcgggaacaa ctccagccag tcagccgcca gcccggtcgc cggctgcgcc
    11341 gccggcaaga cttcaggctc gctggatgcc gcacgctcaa ctgcatcaaa atcataatca
    11401 aacgattcgg cgtccatttc gacataatcg taatcaccca gcggcggctc atcgtcatcc
    11461 gcatcaacca gcggcacagg ctcggccggt tgcgattcaa gcactgcggg cggcgccgcc
    11521 tcagtaatca cctcgggagc aatggccacg acaggctccg aaacttcagc aagcggcgct
    11581 cgaaccggct cttcccacgg cagatcaaca acagcagcga caacaggtgc aggtgcaggt
    11641 gcaggtgcag gtgcaggtgc aatttcgacc ggcgaaggct gcaccgcaac cacttcgaca
    11701 aactcaggat ctgcctgcat ctgcggcata acggcagtag gtatctcagg caccgccaca
    11761 ggcgcgaccg ggacctcctc tataacaggg gccggctgcg gcgcaggaga agtcgcaaca
    11821 ggagcactaa ccggtgccac aatagccgca ccagccactg ggttggtcgc gggatcagca
    11881 gtggcctggc taatccccag cggctttagt gccaccctag gtgcatcgtg ggtatccgcc
    11941 gggcggaagg ccaacatacg caacagcacc atctcaaagc cgctacgtgg gtcgggcgac
    12001 aacggcaaat cgcgtcgccc aatcaacccc atctgataat aaaactgcac gtcttcgcta
    12061 ggcagaaccc tggccagctc aagcactcgc tcacgatccc cctggccgtt atccaccgcc
    12121 tcgggcagcg cctgggcaat ggctactcgg tgcaacacat taagcatctc ggacagtacg
    12181 ccattccagt caggcccctg ctcagccaga tgacgcaccg cttcgatcaa accgcgcgca
    12241 tcaccctcca acagggcgtg cagcacaccg tagacctggc catggtcgag cgtgccaagc
    12301 atggcacgca catcggcggc cagcacctta ccctcaccaa aggcaatcgc ctgatcggtc
    12361 aaactcatgg catcgcgcat ggagccgtcg gccgcgcgac cgagtaacca gagcgcgtca
    12421 ttctcgaacg ggatattttc cgcagccaga acatgggtga ggtgatcaac caccctctcc
    12481 ggcggcatat tcttcagcga gaactgcagg cagcgtgaca acacggtgac cggcagcttc
    12541 tgcggatccg tggtcgccag gaggaatttg acgtggggcg gtggctcttc cagggtcttc
    12601 aacagcgcgt tgaaggagct ggtggagagc atgtgcactt cgtcgatcag gtagaccttg
    12661 aagcgcccac ggctcggtga gtactgcacg ttgtcgagca gctcgcgggt atcttcgacc
    12721 ttagttcggc tggcggcatc gacttcgatc aggtcgataa aacggccttc atcaatctct
    12781 ttgcaaatcg aacagatacc gcaaggcgtc gagctgatgc cagtctcgca attcaggcac
    12841 ttggcaataa tccgcgcaat cgtggtttta cccacaccac gggtgcccgt gaacaaatag
    12901 gcatggtgca gacgctggct atcgagcgca ttgatcagcg ccttgagcac atgcgtctgg
    12961 ccaaccattt cgttgaacga gcgcggacgc cacttgcgtg caagaacctg ataactcatc
    13021 gaaacccgtc acgagaggaa agcaaaagtg gggctaatcc taacggagca tggggcaaat
    13081 tgcatccgat gcgcagccta cgaatggcca gcctgcagca ttgtttacaa ataaactggc
    13141 gaaaaattaa ccagacgggt atcatccgcc ccgttcgtac cacgctgtaa gtcaatcagg
    13201 ccttttggcc ccctcgatgg tttccaggta agagcgcatc acccacgggt aatgccgcac
    13261 agctctaacc catatcccaa cctgctgact gatctggccg tcccgcgcaa gcgctgactc
    13321 aagcccgcct ccgacgtacc gacccgcact ccgtaaccat tatgggaact tgtgcgcgca
    13381 gtccagctcc tatctgcaca tgcaatcgac acattaagtc gacagcctgg agctttattt
    13441 agcgcaatga cgcgcccgct gttttaggaa acacccgaaa aatgaacact caacaccaaa
    13501 ttcaggatgc cattcgcacc ctctccagtg catttgcacc gtttgattgc cgcatcctgg
    13561 ctgctcgcaa aggcagcttc agcttcacta tcgtcaacga tgccggcatc gccaagcaca
    13621 gtcagcgcct gtacccaggc cagtacagcg gcgcacacct tgagcaagtt atcgagcgcg
    13681 cccggcagtc actgactgcc tgattaacga agtccgcgaa ccatcgggca tcttcgatgt
    13741 cagaagctat caccctcagg atggagtacg gtcatggaca acattaatcg cgaactcgtt
    13801 gaacagctgt ttgccccgtt gcgcgctaca ttcagcccgc cccgacccga cggtgggatt
    13861 gttctatccc tgctggatgc cagcgacgcc accgcctaca gccgcgtact cagcgcagct
    13921 caacgcacag actcgcaagc cttcgcccaa agcctggaag acatccgcct ggaacttgcc
    13981 atgcgctccg gcagcatccc agcggatatg cgtaaagcat tgaaagaaca agacagcgtg
    14041 cttagctacc acacaagcta agcaccttag ccgccctgcc gggcagcaga gcggccgtct
    14101 tctacattcc cactcaagta cctcgctgcg tgaaagccat tgcgccgctt tcaaaatcgg
    14161 ctggggctga actcagccag aaatacagga ctacttcagc cccccctaag cgactccagt
    14221 gccgcatagg ccccaaacgc cgtttgcatc acgtgatcct taagcagttg cgacgcctcc
    14281 tgacgcttgt tctcgctatc gcagctaaat gcctgaaccg gctgcttgga atgctgcccg
    14341 acggccgcaa agactagctt gcactgatca acattaagtc cgaagaacgt cgccagtctg
    14401 ccagccatgg cgtcgggcaa ctcattgtag ttaaccacca ccccggaaaa ttcctggcag
    14461 cgctccagcc ccttctgtaa cagtttcccc aggcgtcgag aaataaagtc ttccgagcta
    14521 ctgaacggta ttgcgtcgtc cagtacgctc gcgccgatca tccccggcac catgtgcatg
    14581 ccggggcggc gcaggtggga cacggcaatt tccaatggat cgcggtacac gaataaccag
    14641 ggtgtagtag gaaagcaggt gagcagcagc ggcaactccc caatattcca ggcatcgagt
    14701 ttaatcacca ggcgctgttc caggcctcgg cggcgctgcc catatgccga catgagcccc
    14761 ttgagcgcca tgcaccgttc ggcgctcgcc atgtcgctgc gcagcagggt atccagaggc
    14821 ggcggttcgg aaatgacgat gtgctcgtcc agttgcgcaa gcatctggct gattaaggtt
    14881 gagccgcaac gagaggcatg gaagataaag actgcgggcg ccaacccagg gcgttgttgt
    14941 tgccactgac caagcgcgct caagggcgtc tggcgacgga acgcctggtt gaagggcagg
    15001 cgcagagcct cttccacagc atcaccgaag aagggcttgg ataatcgcat atcgccgaac
    15061 cagcaccagt caacctgcca ccggcctgcc tcctgccaca cgcggatcgg cagccagccg
    15121 tccaggttca gactttccac tgctcgctcc actgccggcg accattgcgc atggctgcct
    15181 gtaatgcctg gcgcgtcaag aacagccctt gctcagcggc caacctgagg gcgcactcaa
    15241 taaacgcctc tacatcctgc aagccttgca aggtctgagt cagttggggg ctagtttcta
    15301 cacagcgctg aaagcgcacg aagtcgcttt gtgcgtgacg agctgcgggt gtgcaggcca
    15361 gcccgtcggt gatctgctgc agcaaccatg gctcttgacg gcaatcgagc accaggtgaa
    15421 tgcgcacttg agtacctcga ttatcgacgc gatgagggcg cgacaagtcg agaaaccaac
    15481 actcacctgc ggtcatcggg atgcgttgct catccagcca gaaatccacg cctggcgggc
    15541 tgagcagcgg aatgtgcagg cgtagatcgg cgtccggccc gcccaggtcg tagtcacggt
    15601 gctcgtggat gcgcccacca ggcccaaggc gtagcagacg ggccgagaca atatgcaaag
    15661 gccagtcgca tagcgcctgg caccagcgag catctgctga ccaaggtgcc cggtgcatgg
    15721 gctggccgcg cccaggagac agctcggtta aggcatcagc cggcgtaatc aacgccacac
    15781 cgctccagtc gccgctgtaa taggcgctat tgaagtgccc ttgccaacga tcgtgctcaa
    15841 tggctgccag tgcctgcaac agcaaaggca ggtccactgg cagcggcaga cgggagaacg
    15901 ccgggcgtgt catgggtgca ggctgttact gaggcaacgc tgccatgggt acgccgatat
    15961 cgccctcagc ccaggccgct tcgcggttgg ccaatgccgt ggcaatcgat tgcacctcgg
    16021 tcgattgcac ctcatcgggc agcgggtcaa gaccggcgct ggcgaagatc tggccatagg
    16081 cgttgcgcag atcggcatag gacaggtccc tgcgcaagtc agcctgcaag gtattcagtt
    16141 caccttggat cagctccagc tcgcccagac ctgcggcctg ataacggttg cgcaactggc
    16201 caacgatttg cccatcgata tccgacagct gttggttggt cttaaattga cgcaacgctt
    16261 cgcggtagtt ggcattggcc acgtagagct gcgcgagcac ggcaatcgac atggcctgtc
    16321 gccgagccgc agcgacttct tcacctgcct tggccacatc aattgcggca ggcgcagaga
    16381 tcacgttgaa cagattccag gtgactttca cgccgtagtc gatccagcct tgctccacca
    16441 ggaaggagtt gctgtcgtaa tgccccccgg cggagaactc cagccctggc aacaggcgca
    16501 acatagcttt acgggtttcg gcagcagtaa tacgtgtctg ataatcctgt tcgcgcagtt
    16561 ccgggcggct agtcagggct tcctgctcaa ggctcgccaa gtctaccttg agctccggga
    16621 tgacgtattc atccggggta gccagggtca actgcgtgcc caggggtagg ttgaccagag
    16681 ttgccagttc agttttggcg agggatagcg cccgccgttg ctcctcaagc tgacgggttg
    16741 cctggatcag cgagcgttga tagcccaggg cctgaaccgg atcaccaatg cgttgttcgc
    16801 tcatactctg gctgttgtcg cgggccgtct cgacccgggc catcagactg tcgatctgct
    16861 tgagcaggcg ttcggcagcc atggcgcgcc agtaggcgga gcgcacatcc tggacgatgg
    16921 tgttgatcac tttgcgtcgg cgttcctgca caatcagacg ctggtcgccc gcctgtttgg
    16981 cgctgatata gctcacgcca aagtcgagca cgttccagac catggtcaga tccgccactt
    17041 cacggtcacg gtcctgtgag gtcgagggtt ccaacgactg ggtaccggtt tccacgcttt
    17101 cactgctcga tgcactgacg ttattgcgac ctacataacc tgcgtccaac gccatgcgcg
    17161 gcagcatgtc gaaactggcg aggtcgagct gccgtttggc caacgcctct tccatgattt
    17221 tcaggcgacc ctcgaggttg tacttcaccg cccgggccat ggcctggtgc agggtcagcg
    17281 gcccactgag gggctcctgg tctttgtaca tattctgcag atcagtccgg gcacgctctt
    17341 cactgacgct gcgttcgatg ggctcactgg tgaccgcgca accgctgaca accaacgcca
    17401 gcagactcat gccgaataac ttctgacttt tgttcattcc ctggattacc cccgaatgcc
    17461 ccagctttta aaaattatca ggcctgtact tggctgatgc cgacctgttg taacgcagct
    17521 gccaggttgt tgacctgacg ttgttccgtg tctttaaggt gttgcagttg ctggcccagg
    17581 gtgggtgcgc cgaataacaa cccgggcacg ccttgggccc catcaccgcc ctgcatcgat
    17641 tggttgccga agacgtccat tgagtcgctc tcggacgcga agtcctggct gaaaagaccc
    17701 gaaatcgtac tgccgccaaa cactccgccg tcgcctccac cgaagccgag gaaaccctgg
    17761 gcggcaccgt tacctgccgc gtcgctgctg aaggcctggg caataaagct cggtgccagt
    17821 gcgccgcggt tgataaagat gtcgccaatc ggccgaatgc ctccgcccac gtcgcgctgt
    17881 tcaaacagcg gcgggaagcc cagcggcgag ccaagatcac cggttggcgg ggtaaagacg
    17941 ataggttgca acggcacctc agccggaggg acaaccgtga cagggtctga ggtcagaaac
    18001 tccggtggag gcttcacctc gttaggcgcc actgtatcga ttacatagtt gttggatacc
    18061 gccacgctcg ctccggcatt actcgccaga tcgctgacat tactggtatc cagagcaatg
    18121 aaattgctcg gatcggtaag gtctgcggtg ggggtgaagg ttgctgtcca ggtcaggccg
    18181 ccgtcactgc tgctcagatt gctcagttca ccgttggtaa cgctgagatc cgacaagtcg
    18241 aagtcactta ccgcttcgcc gaaggtgatg gttacctggg ttgtctcgcc cacactcaag
    18301 ttcggatcgg ccaccacaat agttgcggtt ggccgggtgg cgtcgagcgc gtagttggcg
    18361 gagatcgcga tgcctgcgcc tgcgttgccg gcgatatcct gcacattgct gctgtcgagc
    18421 agaattaggt tagtggtgtc gttgactccc gcagagggtg tcaaggtcgc cgtccaagtc
    18481 aggccgccgt cactgctggt gaggttggac agtacgccat tggcaacgct gaggtccgac
    18541 agatcgaaac cggtcactgc ctcagtgaag ccgatggtta cggtggtggt ctggccgatg
    18601 cctaaccggg tgtcagccaa ggtgatgctg gcacctgggc gaatggtgtc gaccacataa
    18661 ttgttggaat cggtgttgcc gattccagcg ttgcccgcga tgttgattaa gccggtattg
    18721 tccagcgtga tcatgttgct ggtatcggag atgttgctgc tcgggctgaa ggtcgctgtc
    18781 caggtgacgc caccgtcggt gctgctcatg gagctgaggg tgccgttgct gacggtcagg
    18841 tctgagttgt cgaaaccgct gaccgcctcg gagaaggtga tggtcaccaa tgaggtttct
    18901 ccgatgatca gtgacgtatc ggcgaccacg atggtggcgg tcggcacctg ggtatcgatg
    18961 gcg
//
LOCUS       DASRUM010000261        11717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1966493, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000261 DASRUM010000000
VERSION     DASRUM010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 11717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11717
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1966493"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..814
                     /locus_tag="VFF22_17735"
     CDS             41..814
                     /locus_tag="VFF22_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HZX18696.1"
                     /translation="MTELNIVALVSVGAHPTSGRPRRAEQDARAVELGLRLAGERLQV
                     LHAGDPQQEALRAYLGMGLDQIKILEQPAHSDALPVLAEYLADSSAQLVLTGSQAETG
                     EGSGMLPYLLAEQLGWPLVVGLAAVEQVSNGVAQVLQALPRGQRRRLQVRLPFVASVD
                     NAAPTARQSAFGPARRGLLTAEEVELVADPLFASAQLQPAKPRPKRLKVIKAKTGAER
                     MKAATAKASGGGGQVLKDVSPEAGAEAIFKLLLEEGVLR"
     gene            1100..1351
                     /locus_tag="VFF22_17740"
     CDS             1100..1351
                     /locus_tag="VFF22_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18697.1"
                     /translation="MMHADLIDQEDFRERLVALGLSIPSGVTAEQACEHAVSGLSPER
                     AVALRRLVEELLGGSAMLLPNVREAISRTLLPALVPRPS"
     gene            complement(1472..2515)
                     /locus_tag="VFF22_17745"
     CDS             complement(1472..2515)
                     /locus_tag="VFF22_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HZX18698.1"
                     /translation="MPDLSQQFASDNYSGICPEAWAAMAEANQGHDRAYGDDQWTARA
                     ADHFRQLFETDCEVFFAFNGTAANSLALAALCQSYHSVICSETAHVETDECGAPEFFS
                     NGSKLLLAKTENGKLTPEAIREIALKRQDIHYPKPRVVTLTQATEVGTVYSPDEVRAI
                     SAVCRELGLHLHMDGARFSNACAFLGCSPADLTWKAGVDVLCFGGTKNGMAVGEAILF
                     FNKDLAEDFDYRCKQAGQLASKMRYLSAPWVGILQDGAWLRYAQHANRCARLLAELVS
                     DIPDVSLMFPVQANGVFLQMSEPALEILRGQGWRFYTFIGAGGARFMCSWDTDEARVR
                     QLAADIRAAMAAS"
     gene            2788..3081
                     /locus_tag="VFF22_17750"
     CDS             2788..3081
                     /locus_tag="VFF22_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18699.1"
                     /translation="MNNRHLIAFFAPLAFALPAAAEWSTDATAEFTRECVASAPSDHS
                     EAQLKAYCECAADKISSEFTEDELQAMGRQSPPDPELQRRLLNASSSCDSKLQ"
     gene            3147..3911
                     /locus_tag="VFF22_17755"
     CDS             3147..3911
                     /locus_tag="VFF22_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HZX18700.1"
                     /translation="MPTPSPTAPGATRDAALDLIKWLALASMLIDHLRHVWPQLYAVY
                     IPGRLAFPLFCLAIAANLVRPALARPEGGGRAIPLRYLGLLLGFALISEWPYRLLVQS
                     PQSLNVMPTLALGLLIASGMQYPHGQRRWLAIGALLLAVVGHPWLMFGLAGALLPAAF
                     VYALRRPNPGWIWPVLLCLIANYWPPFYVDAMRGDPFAWTVILSCAGAPLFGLWLLRQ
                     PLTLPVPPVRRWAYLFYPGHFLVLVAMREWLVMGAS"
     gene            4128..5381
                     /gene="glyA"
                     /locus_tag="VFF22_17760"
     CDS             4128..5381
                     /gene="glyA"
                     /locus_tag="VFF22_17760"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZX18701.1"
                     /translation="MFSKHDQIQGYDDELLSAINAEESRQEHHIELIASKNYTSQRVM
                     EAQGSGLTNKYAEGYPGKRYYGGCEHVDVVEQLAIDRAKALFGADYANVQPHSGSSAN
                     SAVYLALINAGDTILGMSLAHGGHLTHGAKVSSSGKLYNAVQYGIDTTTGLIDYDEVE
                     RLAVECQPKMIVAGFSAYSKTLDFPRFRAIADKVGALLFVDMAHVAGLVAAGLYPNPI
                     SFADVVTTTTHKTLRGPRGGLILARANAEIEKKLNSAVFPGAQGGPLMHVIAAKAVCF
                     KEAMEPGFKTYQQQVIKNAQAMAKVFIERGYDVVSGGTDNHLFLVSLIRQGLTGKDAD
                     AALGRAGITVNKNAVPNDPQSPFVTSGLRIGTPAITTRGLKEAQSIELAGWICNILDH
                     LGDADVEAQVATLAAGLCADYPVYR"
     gene            5499..6749
                     /locus_tag="VFF22_17765"
     CDS             5499..6749
                     /locus_tag="VFF22_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HZX18702.1"
                     /translation="MQRYSGFGLFKHAFSHHENWQRMWRNPTPKPVYDVIIVGGGGHG
                     LATAYYLAKQFGVKNVAVIEKGWLGGGNTARNTTIVRSNYLWDESAHLYEHAMKLWEG
                     LSQDLNYNVMFSQRGVYNLCHTLQDMRDSERRVSANRLNGVDGELLDAKQVAEEIPYL
                     DCSKNTRYPIMGSTVQRRGGVARHDAVAWGYARAADALGVDLLQQTEVIGFRKENGVC
                     IGVETNKGFIGGKRVGVVTAGNSGHMAGLAGFRLPLESHPLQALVSEPIKPIIDSVIM
                     SNAVHGYISQSDKGDLVIGAGIDGYNGYGQRGSYGTIEHTLQAIVEMFPVLSRVRMNR
                     QWGGIVDTSPDACPIISKTPVPNLFFNCGWGTGGFKATPGSGHVFAASLAKGEMHPLA
                     KPFSMDRFHTGALIDEHGAAAVAH"
     gene            6762..7088
                     /locus_tag="VFF22_17770"
     CDS             6762..7088
                     /locus_tag="VFF22_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003161125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HZX18703.1"
                     /translation="MLHIFCPHCGELRSEEEFHAKGQAHIPRPLDPAACTDAEWGEYL
                     FFRDNPRGIHHELWVHSAGCRKYFNATRHTVTYEILETYKIGEKPSVTAAGTAEKKAI
                     DQGVTA"
     gene            7085..10105
                     /locus_tag="VFF22_17775"
     CDS             7085..10105
                     /locus_tag="VFF22_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit alpha"
                     /protein_id="HZX18704.1"
                     /translation="MSQVNRLATGGRVDRSQPLTFSFNVQSYECFAGDTLAAALLANG
                     VDIVGRSFKYSRPRGIVAAGAEEPNAVLQIGSTEAAQIPNVRATQQALYAGLVASSTN
                     GWPSVNTDLMGILGKVGGKMMPPGFYYKTFMYPQNLWLTYEKYIRKAAGLGRAPTAVD
                     PDSYDYMNQHCDVLVVGAGPAGLAAALAAARSGARVILADEQEEFGGSLLSTRETLDG
                     KPAAEWAAQVIAELSGMPEVTLLPRSTVNGYHDHNFLTIHQRLTDHLGEIAPLGMVRQ
                     RMHRVRAKRVVLATGAHERPLVYANNDVPGNMLADAVSTYVRRYGVAPGKQLVLSTNN
                     DYAYRVVLDWLDAGLQVVAVADARSNPRGSWVEEARKRGVRILTGSAVVEAAGSKRVT
                     AARICAIDVQSHKVSSPGEVLDCDLIVSSGGYSPVVHLASHLGGRPTWREDILAFVPG
                     EGGFQKRLCAGAVNGVFSLGDALADGFEAGVSAAAEAGFKAVSASLPQVAVRMEEPSL
                     ALFQVPHDKPTARAPKQFVDLQNDVTAAGIELATREGFESVEHVKRYTALGFGTDQGK
                     LGNINGLAIAARSLGISIPQMGTTMFRPNYTPVTFGAVAGRHCGALFEPKRYTAMHSW
                     HLQNGAEFEDVGQWKRPWYFPKAGEDLHAAVARECLAVRNAVGILDASTLGKIDIQGP
                     DAREFLNRVYTNAWTKLDVGKARYGLMCKEDGMVFDDGVTACLADNHFLMTTTTGGAA
                     RVMEWLEIYHQTEWPELKVYFTSVTDHWATMTLSGPNSRKLLSEVTDIDLDKEGFPFM
                     TWKEGQVGGVPARVFRISFTGELSYEVNVQADYALSVWEKIIEAGKKHGLTPYGTETM
                     HVLRAEKGFIIVGQDTDGSVTPDDLNMGWCVGRTKPFSWIGWRGMNREDGLREDRKQL
                     VGLKPVDSNKVLPEGAQLVFDPKQSIPMSMVGHVTSSYMSAALGYSFAMGVVKGGLKR
                     IGERVFAPLADGSLIEAEIVSSVFYDPKGDRQNV"
     gene            10098..10730
                     /gene="soxG"
                     /locus_tag="VFF22_17780"
     CDS             10098..10730
                     /gene="soxG"
                     /locus_tag="VFF22_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:1901053 - sarcosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HZX18705.1"
                     /translation="MSNLINVYQQRPAAAHAESPLFHAGLEQLAGKGAASAGVTLREK
                     KLLGHLTLRGDGKDPAFAGGVHKALGLELPVALTLVAAGDTSLQWLAPDEWLLIVPSG
                     SEFSVEQRLREALDGQHISIVNVSGGQTLLELSGPKVREVLMKSTSYDVHPSNFPVGK
                     AVGTHFAKSQLVIRHTAEDTWELLIRRSFSDYFWLWLQDASAEFGLAIKA"
     gene            10818..11681
                     /gene="purU"
                     /locus_tag="VFF22_17785"
     CDS             10818..11681
                     /gene="purU"
                     /locus_tag="VFF22_17785"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HZX18706.1"
                     /translation="MSRTPDTWILTAHCPSVLGTVDAVTRFLFEQRCYVTEHHSFDDR
                     LSARFFIRVEFRQPDYFDEQAFRAGLDQRLTAFGMQVELTPPGYRAKVVLMVSKADHC
                     LNDLLYRQRIGHLAMDVVAVVSNHPDLEPLARWHDIPYHHFPLDPNDKPAQERKVLQV
                     IEDSGAELVVLARYMQVLSPELCRKLDGWAINIHHSLLPGFKGAKPYHQAYEKGVKLV
                     GATAHYINNDLDEGPIIAQGVESVDHAHYPEDLIAKGRDIECQTLAKAIGYHLERRVF
                     LNASRTVVLHA"
BASE COUNT         2120 a         3646 c         3749 g         2202 t
ORIGIN      
        1 aacaccgact tccggattgc atccgggcta cgggggatgc atgactgagt tgaatattgt
       61 tgcgctggtt tcggtcggcg cgcatcccac ctccgggcgc ccgcgccgcg ccgagcagga
      121 cgcccgcgca gtcgagctgg gtttgcgtct ggccggtgag cgtttgcagg tgctgcatgc
      181 cggcgatccg cagcaggagg cgttgcgcgc ttacctgggc atgggccttg atcagatcaa
      241 gattcttgag caaccggcgc acagcgatgc gctgccggtg ctggccgagt acctggccga
      301 ctccagcgca cagctggtgt tgaccggcag ccaggcggaa accggtgaag gctcgggcat
      361 gctgccttat ctgttagcgg aacagctcgg ttggccactg gtagtggggc tggcggcggt
      421 ggagcaggtc agcaatggcg tggctcaggt gctgcaagcc ctgccgcgcg gtcagcgccg
      481 gcgcttgcag gtgcgtttgc cgtttgttgc cagtgtcgat aacgcggcac caactgcccg
      541 gcagagtgca ttcggcccgg cccggcgtgg cctgttgacg gccgaggagg tggaactggt
      601 cgccgacccg ctgtttgcca gcgcgcaact gcagccggcc aaaccccgac ccaaacgcct
      661 taaggtgatc aaggccaaga ccggcgccga acggatgaag gccgccacgg ccaaggcgtc
      721 cggcggagga gggcaagtgc ttaaggatgt atcgccagag gccggggccg aggctatctt
      781 caagctgctg ttggaagagg gcgtgctgcg ttaatggcgt tgctatgaat gtgggaggag
      841 gctttcggct ctggcatcct gctgcgccct acctcctgca tccatgcagt cgtagccgcg
      901 acagccatgg ttaaaagctc gccgctaaag cgcctcccac gctcttattc cctgcgtcgg
      961 cggagttagc gtccgctagc taagggcatc acaggtcgta gcccggatga aatccgggag
     1021 gccgcgattc ccggattgca tccgggctac ggttgattgc tgagacattt ttattgccgc
     1081 accactggag ggtttgacca tgatgcatgc cgacctgatc gatcaggagg atttccgcga
     1141 acgcctggtg gcgctggggc tgtcgattcc gtcaggggta acggcggaac aggcctgtga
     1201 gcatgccgtc agtggtctaa gccctgagcg cgcggtagcg ctgcggcggt tggtggagga
     1261 attgctcggt ggcagcgcga tgctgttgcc gaatgtgcgc gaggcgattt cgcgtacgtt
     1321 gttgccggca cttgtaccgc gcccttcgtg aatatttcgg tcatgtagga gtcagcttgt
     1381 tgcggtccgg cgtcccggcg atccaggcca acagcatcgc cagtaagctg gctcctacat
     1441 ggaaaaacgg tttacctgca ccggtattgc cttagctcgc cgccatcgcc gcgcgaatat
     1501 ccgctgccag ctggcgtacc cgcgcttcgt cggtgtccca agagcacata aagcgtgcgc
     1561 cgccggctcc gataaaggtg tagaagcgcc agccctggcc gcgcaggatt tccagcgccg
     1621 gttccgacat ctgcaaaaac accccgttgg cctgcactgg gaacatcaaa ctgacatctg
     1681 gaatatcgct gaccagctcg gccagcaggc gcgcgcagcg gttggcgtgc tgggcgtaac
     1741 gcagccaggc gccgtcctgc agaatgccga cccagggcgc ggacaggtag cgcatcttcg
     1801 aggccagctg accggcctgc ttgcagcggt agtcgaagtc ttccgccaga tccttgttga
     1861 agaacaggat cgcctcaccc acggccatgc cgttcttggt gccgccaaaa cacagcacat
     1921 cgacgccggc cttccaggtc aggtcggccg ggctgcagcc gagaaaggcg caggcgttgg
     1981 agaagcgcgc gccgtccata tgcagatgca ggcccagttc gcggcatacc gcactgattg
     2041 cccgcacttc atcggggctg tagacggtgc cgacttccgt ggcctgggtc agggtgacta
     2101 cccgtggctt ggggtagtga atgtcctggc gcttgagggc gatttcacgg attgcttcgg
     2161 gggtcagctt gccgttctcg gtcttggcca gtagcagttt ggagccgttg gagaaaaact
     2221 ccggcgcgcc acattcgtcg gtttcgacgt gggcggtctc cgagcagatc acgctgtgat
     2281 agctctggca cagcgctgcc agggccaggg agttggcggc ggtgccgttg aaggcgaaga
     2341 acacttcgca gtcggtttca aacagctggc ggaagtggtc ggcggcgcgg gcggtccact
     2401 gatcatcgcc gtaggcgcgg tcgtggccct ggttggcttc ggccatggct gcccatgcct
     2461 ccggacagat accggagtag ttatcgctgg cgaactgttg cgaaagatcg ggcattgcta
     2521 gttccttcta tcggttgccg agcgggccgg cgaatgggtt tactttacct gcgcatgcgc
     2581 ggcccggcgg acgttgtggc gacatcttgt ttattccagg cgctattggg cttggtaagc
     2641 agtcttgacg gcgcgacggc ttgagacggc acagcactaa gccgcggcgc tggcagcgga
     2701 ataaatgaaa ggaactcacg gcgccgggcg gcctcagaaa tagggactcg gcaggcctgc
     2761 cgtgtcacgc ctgaatagga agctcgtatg aataaccgac acctgatcgc ttttttcgct
     2821 ccactggcct ttgccttgcc ggcagctgcc gaatggtcga ctgacgcgac tgccgagttc
     2881 acccgcgagt gcgtcgccag cgccccgtcg gaccacagcg aagcgcagtt gaaggcttac
     2941 tgcgaatgcg ccgctgacaa gatcagcagc gagttcactg aagacgaatt gcaggcgatg
     3001 gggcgtcaat caccgcctga tcccgagctg cagcgtcgtc tgcttaacgc atcgagcagt
     3061 tgcgacagta aattgcagta atgggggcgg catggggagc ggcagtgggg taaggtcgaa
     3121 cacgcccttt gcaggctgag gccagcatgc cgactccttc ccccactgcg ccaggcgcga
     3181 ctcgtgatgc cgcgctggac ctgatcaagt ggctggcgtt ggccagcatg ctgatcgatc
     3241 atctgcggca tgtctggccg cagctctacg cggtttacat tccaggccgt ctggccttcc
     3301 cgctgttctg tctggcgatt gctgcgaacc tggtacggcc tgccctggcg cggccagagg
     3361 gtggcgggcg tgccatcccg ttgcgctacc tgggattgct gctgggcttc gcgctgatct
     3421 ccgaatggcc ttaccgcctg ctggtgcaaa gccctcagtc gcttaacgtt atgcctaccc
     3481 tggcgcttgg tttgctgatt gccagcggta tgcaatatcc gcatgggcag cggcgctggc
     3541 tggcgattgg cgcactgctg ctggcggtgg tcggccatcc atggctgatg ttcggtcttg
     3601 cgggtgcgtt gctgccggcc gcgtttgtgt atgcgctaag gcgacccaat cccggctgga
     3661 tctggcccgt gctgctgtgc ctgatcgcca attactggcc gccgttctat gtcgacgcga
     3721 tgcgcggcga tcctttcgcc tggacggtca ttctgagttg cgcgggcgcg ccgctgttcg
     3781 gtctctggtt gttgcgtcag ccgctgacgt tgcccgtacc gccggtacgg cgctgggcct
     3841 atctgttcta tccggggcac tttctggtgc tggtggccat gcgcgagtgg ctagtgatgg
     3901 gcgcctcgta acccggcggt aatccgcgcg cgattggccg gcgcaagatt tcctcgtatt
     3961 gcctcctgct atgagctgcg taattgcgac ctgtgcatgt cgcaaacgca atctcccgtg
     4021 gcgtgcctag gcatctgacc gatcagggcc agtcatacca tcggctcaca agggcccagc
     4081 ccgctatcga cacagatgcg ctgcccaggc gccgcaggag atccgcgatg ttcagcaagc
     4141 acgaccagat ccagggttac gacgatgaat tgctcagtgc gatcaacgcc gaggaaagcc
     4201 gtcaggagca ccatatcgag ctgatcgcct cgaaaaacta caccagccag cgagtcatgg
     4261 aagcccaggg cagcggcctg accaacaagt acgccgaagg ctatccgggc aagcgttatt
     4321 acggcggctg cgagcatgtc gacgtggttg agcaactggc gattgatcgc gccaaggcgc
     4381 tgttcggcgc cgattacgcc aacgtgcagc cgcattctgg ctcgtcggcc aacagtgcgg
     4441 tgtacctggc gctgatcaat gccggcgaca ccatcctggg catgagcctg gcccacggcg
     4501 gccacctgac ccacggcgcc aaggtgtcgt cctcgggcaa gctgtacaac gccgtgcagt
     4561 acggcatcga caccaccact gggctgatcg attacgacga agtcgagcgt ctggccgtgg
     4621 agtgccagcc gaagatgatc gtcgccggtt tctccgccta ctcgaaaacc ctggatttcc
     4681 cgcgttttcg cgccatcgct gacaaggtcg gggcgctgct gtttgtcgac atggcccacg
     4741 tcgccggcct ggtcgctgcg ggcctgtacc ccaacccgat ttcctttgcc gacgtggtca
     4801 ccaccaccac ccacaagacc ctgcgcggtc cgcgtggcgg gctgatcctg gctcgcgcca
     4861 atgccgagat cgagaagaaa cttaactcgg cggtattccc cggcgcccag ggtggcccgt
     4921 tgatgcatgt tatcgcggcc aaggcggtgt gcttcaagga agccatggaa ccaggcttca
     4981 agacctacca gcagcaggtg atcaaaaacg cccaggccat ggccaaggtg tttatcgagc
     5041 gcggctacga cgtggtctcc ggtggcaccg acaaccacct gtttctggtc agcctgatcc
     5101 gtcagggcct tactggcaaa gacgccgatg cggccctggg gcgtgccggc atcacggtca
     5161 acaagaacgc cgtgccgaat gacccgcagt cgccgttcgt gacttcgggc ctgcgtatcg
     5221 gcactccggc catcaccacg cgcggcctca aggaagcgca gagcatcgag ctggccggtt
     5281 ggatctgcaa catcctcgac cacctgggcg atgccgatgt ggaagcgcag gtggcgactc
     5341 tggctgctgg cttgtgtgct gactatcccg tttatcgctg agcgcaatga tcctgtagga
     5401 gcggggggga cgccgagtcc catgcccgcg attccacggg gttcgcgggc atggcccgct
     5461 cctacggtta ggctttttcg tatcaggagt acccactgat gcaacgttat tccggcttcg
     5521 gcctgttcaa gcacgctttc agccaccacg aaaactggca gcgcatgtgg cgcaacccga
     5581 cgcccaagcc ggtgtatgac gtgatcatcg tcggcggtgg cggtcacggt ttggcgaccg
     5641 cctattacct ggcgaaacag ttcggcgtga agaacgtcgc ggtgatcgag aagggctggc
     5701 tgggcggcgg caacaccgcg cgcaacacca ctatcgtgcg ttccaactac ctatgggatg
     5761 agtcggcgca cctgtatgag catgcgatga aactgtggga aggcctgtcc caggacctca
     5821 actacaacgt catgttcagc caacgcggcg tctacaacct ctgccacacc ctgcaggaca
     5881 tgcgcgattc cgagcgccgc gtcagcgcca accgcctcaa cggtgtggat ggcgaactgc
     5941 tggatgccaa gcaggtggcc gaagagattc cctatctgga ttgcagcaaa aacacccgct
     6001 acccaatcat gggttccacc gtgcagcgcc gtggcggcgt tgctcgtcac gatgccgtgg
     6061 cctggggcta cgcccgcgcc gccgatgccc tgggtgtcga cctgctgcag cagaccgaag
     6121 tgatcggttt tcgcaaggaa aatggcgtgt gcatcggcgt ggaaaccaac aagggcttta
     6181 tcggcggcaa gcgcgtcggc gtggtcacgg ccggtaactc cgggcatatg gccgggctgg
     6241 ccggcttccg tctgccgctg gaatcccatc cgctgcaggc gctggtgtcc gagccgatca
     6301 agccgattat cgacagcgtg atcatgtcca acgccgtgca cggctatatc agccagtcgg
     6361 acaagggcga cctggtcatc ggtgccggta tcgacggtta caacggctac ggccagcgcg
     6421 gctcctacgg caccatcgaa cacaccctgc aggccatcgt cgagatgttc ccggtgcttt
     6481 cgcgggtacg catgaaccgt cagtggggcg gcatcgtcga cacctcgccg gacgcctgcc
     6541 cgatcatttc caagaccccg gtacccaacc tgttcttcaa ctgcggttgg ggcaccggcg
     6601 gtttcaaggc cacgccgggc tcgggccacg tgttcgccgc cagcctggcc aagggtgaaa
     6661 tgcacccgct ggccaagccg ttctccatgg accgtttcca caccggcgca ctgatcgacg
     6721 agcacggcgc tgctgccgtg gctcactaag agggcttacc catgttgcat atcttctgcc
     6781 ctcactgcgg cgaactgcgc tccgaagagg aattccacgc caagggccag gcgcatattc
     6841 cgcgcccgct ggacccggcc gcctgcactg atgccgagtg gggcgagtac ctgttcttcc
     6901 gcgataaccc gcgcggcatt caccacgagc tgtgggtgca ttcggccggt tgccgcaagt
     6961 acttcaacgc cacccgtcac acggtgacct acgaaattct cgaaacctac aagatcggcg
     7021 agaagcccag cgtcaccgct gccggcactg ccgagaaaaa ggccatcgac caaggagtaa
     7081 cggcatgagc caggtcaatc gtcttgctac aggcggccgc gttgatcgca gccagcccct
     7141 gacattcagc ttcaacgtgc agagctatga atgctttgcc ggtgacaccc tggcggccgc
     7201 cttgctggcc aatggggtgg atatcgttgg gcgcagcttt aagtattcgc gccctcgcgg
     7261 catcgtcgcc gctggtgccg aagagccgaa cgccgtgctg cagatcggca gcaccgaagc
     7321 ggcgcagatc cccaacgtgc gtgccaccca gcaggcgctg tatgccggcc tggtcgccag
     7381 cagcaccaac ggctggccga gcgtaaacac tgacctgatg ggcattctcg gcaaggtcgg
     7441 cggcaagatg atgccgccgg ggttctacta caagaccttt atgtacccgc agaacctctg
     7501 gctgacctac gagaaataca tccgtaaggc cgccggtctt ggtcgtgcgc cgaccgccgt
     7561 ggacccggac agctacgact atatgaacca gcactgtgac gtgctggtgg tcggtgccgg
     7621 cccggcaggc ttggcggcgg cgttggctgc tgcacgcagc ggcgcccgag tgattctggc
     7681 ggacgagcag gaagagttcg gcggcagcct gctcagcact cgcgaaaccc tcgacggcaa
     7741 accggccgcc gagtgggccg cgcaggtgat tgccgagctg tctggcatgc ctgaagtcac
     7801 cctgttgccg cgttccacgg tcaacggtta ccacgaccac aacttcctca ccattcatca
     7861 gcgcctgacc gatcacctcg gtgaaatcgc gccacttggt atggtgcgtc agcgtatgca
     7921 ccgcgtacgc gccaagcgcg tggtgctggc caccggtgcc cacgagcggc cgctggtgta
     7981 cgccaacaac gacgtgccgg gcaatatgct cgccgatgcg gtttccacct acgtgcgccg
     8041 ttatggcgtg gcaccgggca agcagctggt gctgtcgacc aacaatgatt acgcctaccg
     8101 cgtggtgctc gactggctcg atgccggctt gcaggtggtg gcggtggccg atgcccgcag
     8161 caacccacgc ggcagctggg tggaagaagc gcgcaagcgc ggcgtgcgca tccttaccgg
     8221 cagcgccgtg gtcgaagccg cgggcagcaa gcgggtcacc gctgcgcgca tctgcgccat
     8281 cgatgtgcag agccacaagg tcagcagccc cggcgaagtg ctcgattgcg atttgattgt
     8341 cagctccggc ggttacagcc cggtggtgca cctggcgtcg cacctgggcg gccggccgac
     8401 ctggcgtgaa gatattctgg ccttcgtgcc aggcgagggc ggcttccaga agcgcctgtg
     8461 cgctggtgcg gtgaatggcg tattcagtct cggtgatgcc ctggctgatg gtttcgaggc
     8521 cggtgtttcg gcggctgccg aagccggctt caaggcggtg tccgccagcc tgccgcaggt
     8581 ggcagtgcgc atggaagagc cttccctggc gctgttccag gtgccgcacg acaagccgac
     8641 ggcgcgtgcg cccaagcagt ttgtcgacct gcagaacgac gtcaccgctg ccggtatcga
     8701 gctggcgacg cgtgaaggct ttgagtcggt cgagcacgtc aaacgctaca ccgcgctggg
     8761 cttcggcacc gaccagggca agctgggcaa tatcaacggt ttggcgattg ccgcccgctc
     8821 gctgggcatc agcatcccgc aaatgggcac caccatgttc cgcccgaact acaccccggt
     8881 tactttcggc gcggtcgccg gccgtcactg tggcgcgctg ttcgagccca agcgctacac
     8941 cgcgatgcac agctggcacc tgcaaaatgg tgccgagttc gaggatgtcg gccagtggaa
     9001 gcgcccctgg tacttcccca aggccggtga agacctgcac gccgccgtgg cccgcgagtg
     9061 cctggccgta cgtaacgcag tcggcattct cgatgcctcg acgctgggca agatcgatat
     9121 tcaaggcccg gatgcccgcg agtttctcaa ccgcgtctac accaacgcct ggaccaagct
     9181 ggatgtgggc aaggcgcgct acggcctgat gtgcaaggaa gacggcatgg tcttcgacga
     9241 cggcgtaacc gcctgcctgg ccgacaacca tttcctgatg accaccacca ccggcggcgc
     9301 tgcgcgggtg atggaatggc tggagatcta tcaccagacc gagtggccag agctgaaggt
     9361 gtacttcacc tcggtcaccg accactgggc gaccatgaca ttgtccggcc ccaacagccg
     9421 caagctgctg agtgaggtca ccgacatcga tctggataag gaaggcttcc cgtttatgac
     9481 ctggaaggaa ggccaggttg gcggcgtacc ggcgcgggtg ttccgcatct cctttaccgg
     9541 cgagctgagt tacgaagtca acgtgcaggc cgactacgcc ctgagcgtgt gggaaaaaat
     9601 catcgaggcc ggcaagaagc atggcctgac gccttatggc accgaaacca tgcacgtact
     9661 gcgcgccgag aagggtttca tcatcgttgg tcaggacacc gacggctcgg tgaccccgga
     9721 cgacctcaac atgggctggt gtgttggtcg gaccaagccg ttctcctgga tcggctggcg
     9781 cggcatgaac cgcgaggacg gcctgcgcga ggaccgcaag cagctggtcg ggctcaaacc
     9841 cgtcgactcg aacaaggtgt tgccggaagg cgcgcaactg gtgttcgacc cgaaacagtc
     9901 cattccgatg agcatggtcg gccacgtcac ctccagctat atgagcgcgg cgctgggtta
     9961 ttccttcgcc atgggtgtgg tcaagggcgg cctcaaacgc atcggcgagc gagtgttcgc
    10021 gccactggcc gatggcagcc tgatcgaagc cgaaatcgtc agctccgtgt tctatgaccc
    10081 gaaaggggat cgccagaatg tctaacttga tcaatgtcta tcaacaacgc ccggccgccg
    10141 cgcatgccga gtcgccactg ttccatgccg gccttgagca actggcgggc aaaggtgccg
    10201 ccagcgctgg ggtgaccctg cgcgagaaga aactgctcgg tcacctgacc ctgcgcggcg
    10261 atggcaaaga cccggcgttt gccggcggcg tgcacaaagc tctgggcctg gaactgccgg
    10321 tggcgctgac cctggtggcc gctggcgaca cctcgctgca atggctggcg ccggatgagt
    10381 ggctgctgat tgtgccgagc ggcagcgagt tctctgtcga gcagaggctg cgcgaagcgc
    10441 tggatggcca gcacatctcc atcgtcaacg tcagcggcgg gcagaccctg ctggaactga
    10501 gcgggccgaa ggtgcgcgaa gtgctgatga aatccaccag ctacgacgta cacccgagca
    10561 acttcccggt gggtaaggcg gtgggtacgc acttcgccaa gtcgcaactg gtgattcgtc
    10621 acaccgccga agacacctgg gaactgctga tccgccgcag cttctccgat tacttctggc
    10681 tgtggctgca ggatgctagc gccgagtttg gactggcgat caaggcctga cggcaataaa
    10741 cattctgtgg gaggggcttt agccgcgact gcttttcgcg gctaaagccc cccacgagtt
    10801 ccaccgaggg cccgatcatg agccgcaccc ccgatacctg gattctcacc gcccactgcc
    10861 cgagcgtgct cggcaccgtg gatgcggtga cgcgctttct gttcgagcag cgctgctatg
    10921 tcaccgaaca ccattccttc gatgatcgcc tgtcggcgcg gttctttatt cgcgtggaat
    10981 tccgtcagcc agattatttc gatgagcagg cctttcgcgc cggcctcgat cagcgtctga
    11041 ctgctttcgg catgcaggtc gagctgactc cgccgggtta ccgcgccaag gtggtgctga
    11101 tggtcagcaa ggccgaccac tgcctgaacg acctgctcta tcgccagcgt attggccacc
    11161 tggcgatgga cgtggtggcg gtagtgtcca accacccgga tctggagccg ctggcgcgct
    11221 ggcacgacat tccctaccac cactttcccc tcgacccgaa tgacaagcca gcacaggaac
    11281 gcaaggtgct gcaggtgatc gaggacagcg gcgccgagct ggtggtgctt gcccgctaca
    11341 tgcaggtgct gtcgcccgag ctgtgccgca agctggatgg ctgggcgatc aatattcacc
    11401 actcgctgct gcccggtttc aagggcgcca agccctatca ccaggcttac gagaagggcg
    11461 tcaagctggt cggcgccacc gcccactaca tcaacaacga cctcgacgag gggccgatca
    11521 tcgcccaggg cgtggagtcg gtggatcacg cccactaccc cgaagacctg atcgccaagg
    11581 gccgcgatat cgaatgtcag accctggcca aggccatcgg ctatcacctc gaacgacggg
    11641 tgtttctcaa cgccagccgc acggtggtgc tgcacgcata gagcctgaat tcaaggcgta
    11701 ggagcgggcc atgcccg
//
LOCUS       DASRUM010000262         5976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1136992, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000262 DASRUM010000000
VERSION     DASRUM010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136992"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="VFF22_17790"
     CDS             <1..1285
                     /locus_tag="VFF22_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZX18707.1"
                     /translation="RHQKVVEEAPCPVMTPELRRAMGEAAVKAAASVNYVGAGTVEFL
                     LDQSGEFFFLEMNTRLQVEHPVTELITGQDLVAWQIRTASGQPLPLKQDEIRLTGHAM
                     EVRLYAENATQNFLPQTGTALRWEPALRDGIRIDHGLVEGQAITPFYDPMLAKVIAYG
                     ATREEARRKLVRAVEDCVLLGVNGNQRFLANLLSHPEFAAGNATTAFIAEHFASDPSL
                     SPLPPKARELAAAAALLFQQSANARAHQPSLSGWRNAGSAPWRFLLKQGEQPFAVELE
                     VLGDGPQPSLLATIGEQQVSLKLLASDGRWATLELDGIRQRLAYHLAGDNLWLYGHNG
                     NLQLTDITHLPVSSAAGAGSGSVKAPMDGAIVEVLVEEGASVSKGQLLVVLEAMKMEH
                     PLKASIDGVVRRIGVSRGDQVKNRQLLVEIEASEI"
     gene            1399..2223
                     /locus_tag="VFF22_17795"
     CDS             1399..2223
                     /locus_tag="VFF22_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZX18708.1"
                     /translation="MSLQGKTLFITGASRGIGREIALRAAREGANIVIAAKSAEPHTK
                     LPGTIHSVAAEVEEVGGKALALQVDVRDENAVKAAMAQAAEHFGGIDALINNAGAIKL
                     VGVETLEPKRFDLMFQINTRAVMVCSQAALPFLKQSSNGHILNLSPPLNMDSKWFAQH
                     GPYTVTKYGMSMLTMGMSEEFKKYGISVNSLWPKTMIATAAIEFELGSRDAFKRARIP
                     AIMADAAHAILSSQGRSITGRLLIDEEILREQGVSEFEHYRFDPQGGALIADLFVD"
     gene            2447..4273
                     /locus_tag="VFF22_17800"
     CDS             2447..4273
                     /locus_tag="VFF22_17800"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HZX18709.1"
                     /translation="MSQADLISPLRFLARLPQHLPRVPRMLRGLYYAGIRNREKNLSL
                     AWALERTAQQHPDRPAVLDESRSLSYAQFNALANRLAWAFKAEGVRHGNVVAVMLENR
                     LEQLAILASLSKLGAVGALVNTTQRGKVLAHSFNLVKPGFMVIGEELREAFEEVAEQL
                     DNSNAPRYWIADQDCLADAGVAPDGWTNLMQLARSQASDNLPDSAKVKMKDPCFLIYT
                     SGTTGLPKASIMSHGKWIKAYGGFGHSGLTLGNQDVLYLTLPCYHNNAVTVCWSAAIA
                     GGAAIALRRKFSASAFWPDVQRYQATCFGYIGELCRYLLNQPECAAEKNNSLRCMIGN
                     GLRPSIWAEFKARFGVEQITEFYASSEGNIGFTNVFNFDNTVGFSPATYAIVRYDLEN
                     DCAIRTAKGFLQKADKGEPGLLISEISAKWPFDGYTDPAKSEAAILRNVFKKGDAWFN
                     TGDLMRDIGCKHAQFVDRLGDTFRWKGENVSTTEVENVLGAFPGVEDAVVYGVEIPGT
                     NGRCGMAALRLSEGQQLDSAALAAHLDAELPVYAAPLFIRLLGEVETTGTFKYKKTDL
                     KQAAYNPSAVQEPLWVRLPGESRFTPLDPSLFAAIGEQVYRF"
     gene            complement(4308..4805)
                     /locus_tag="VFF22_17805"
     CDS             complement(4308..4805)
                     /locus_tag="VFF22_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18710.1"
                     /translation="MLPIRSLALLLTMLSLVACEQSTNPPAKPKVEIAKAQPAQPVVT
                     SKATKAEPAPVVNKPAQPKAAEAKPAAATQTKPKPAVVASKSEPAEPKAPLDLSLNTN
                     VFDPLQPVAPLSDLSIPILPPMFGDKDKTEEPFQLNGKLITNERDDDYWQSVEGAQLQ
                     FEFKQ"
     gene            complement(4967..5698)
                     /locus_tag="VFF22_17810"
     CDS             complement(4967..5698)
                     /locus_tag="VFF22_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18711.1"
                     /translation="MNHDQHRLDEQERQMLAHFRQHSGGEPSAQLDAQILAAASAAAR
                     SAPAAEAKPGLSERLHSWLFGAGGRQRWSVALAGVACLGIGVSLTWRTFEQAPDAFDA
                     VPSAAPMAPAAARMAPQSEPLAESAAVAEEAQRSRAEIQGYSADAAKAEKKLSAGASS
                     QLLREESAADALHDAAPDVSASEKAAEAASSQAEQQLHSELQRLLKLRRDGWTDEADA
                     LLLQLMREHPELDIDAQLQQLQSPE"
     gene            complement(5695..>5976)
                     /locus_tag="VFF22_17815"
     CDS             complement(5695..>5976)
                     /locus_tag="VFF22_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZX18712.1"
                     /translation="EYDEQQHAPHDPQPGPEQQLSLSRDQQRLEAALADLPAEQREVF
                     LLRAHGDLELAEIAELTQIPAETVKSRLRYALQKLRRLLADPAAEELSV"
BASE COUNT         1176 a         1913 c         1780 g         1107 t
ORIGIN      
        1 ccgccaccag aaagtggtcg aggaagcacc ctgcccggtg atgacgcccg agctgcgccg
       61 cgccatgggt gaagcagccg tcaaggccgc cgcctcggtg aactacgtcg gcgcaggcac
      121 cgtggagttt ctgctggatc agagcggcga attcttcttt ctggaaatga acacccgcct
      181 acaggttgag cacccggtca ccgaactgat caccgggcag gacctggttg cctggcagat
      241 tcgcacggcc tccggccagc cgctaccgct caagcaggac gagattcgcc tcaccggcca
      301 cgccatggag gtgcgcctgt acgccgaaaa tgccacgcag aatttcctgc cgcaaaccgg
      361 caccgccctg cgttgggagc cggcactgcg cgatggcatt cgcatcgacc acggcctggt
      421 cgaaggccag gccattaccc cgttctacga tccgatgctg gcaaaggtca tcgcctatgg
      481 cgcgacccgt gaggaagcgc ggcgcaagct ggtacgtgcg gttgaagatt gcgtgctgct
      541 gggggtgaac ggcaaccagc gctttctcgc caacctgctc agccaccccg agtttgccgc
      601 cggcaatgcc accaccgcgt ttatcgccga gcatttcgcc agcgacccaa gccttagccc
      661 gctgccgccc aaagccagag aactggccgc cgctgccgcc ctgctgttcc aacaatcggc
      721 caatgcccgc gcacaccaac cgagcctttc cggctggcgc aatgctggca gcgcgccatg
      781 gcgctttctg cttaagcagg gcgaacagcc ctttgccgta gagctggaag tgctgggcga
      841 cggcccacaa cccagcctgc tggccacaat tggcgagcag caagtcagcc tcaaactgct
      901 ggccagcgac ggccgctggg ccacactgga actggacggc attcgccagc gcctggccta
      961 tcacctggcc ggcgacaatc tctggctgta tggccataac ggcaatctgc agctcaccga
     1021 catcacccac ctgccggtca gcagcgctgc cggcgccggc tcgggtagcg tcaaggcgcc
     1081 gatggacggc gcgattgtcg aggtactggt ggaggaaggc gccagcgtca gcaaaggcca
     1141 actgctggtg gtgctggaag cgatgaagat ggaacacccg ctcaaggcca gcatcgacgg
     1201 cgtggtgcgc cgtattggcg tcagccgtgg cgaccaggtg aaaaaccgcc agttgctggt
     1261 ggaaatcgaa gccagcgaaa tatgaaagtc gtaggttggc gctgagcctg cgaagcccaa
     1321 cgttgggctt cgttgcactc aacaccaacc tacgcatcaa cgctccaagc gaataacaat
     1381 aaagtcggga gaacgcttat gtcactgcaa ggaaaaaccc tgtttatcac cggtgcaagc
     1441 cgtggcatcg gccgtgaaat cgccctgcgc gcagcgcgtg agggggccaa tatcgtgatt
     1501 gcggcaaaga gcgctgaacc tcataccaag ctgcccggaa ccatccattc ggtggccgca
     1561 gaagttgaag aggtcggcgg caaggccctg gccctgcagg tcgatgtgcg tgacgagaac
     1621 gcggtaaaag ccgccatggc ccaggcggcc gagcactttg gtggcatcga tgcgctgatc
     1681 aacaacgccg gtgcgatcaa actggtcggg gtggaaacac tcgagcccaa acgctttgac
     1741 ctgatgttcc agatcaacac ccgcgcggtc atggtctgca gccaggccgc cctgccgttc
     1801 cttaagcaga gcagcaatgg ccatatcctc aacctatcgc caccgctgaa tatggacagc
     1861 aaatggtttg cccaacacgg cccctacacc gtcaccaagt acggcatgag catgctcaca
     1921 atgggcatga gcgaggagtt caagaagtac ggtatcagcg tcaactcgct gtggccgaaa
     1981 accatgatcg ccaccgccgc catcgagttc gaactaggca gccgcgacgc cttcaagcgc
     2041 gctcgcatcc cggccatcat ggccgacgcc gctcacgcca ttctgtcgag ccaaggccgc
     2101 agcatcaccg gacgcttgct gatcgacgag gaaattctgc gcgaacaggg tgtcagcgag
     2161 ttcgagcact atcgtttcga cccgcaaggc ggcgcgctga ttgcggatct gttcgtcgat
     2221 tgatcatctc ttagggccac ttcagccgtg aagtgctcgg ctcggcatag cgccactcac
     2281 ggctgaggtt cccagcagaa tgacttgctc tgctggaagg gcttgggcca cgacaatcta
     2341 tttgccgatc tgcccggtca agcttgcgcc agggccatgg cagacgtgca ttcgcgcgac
     2401 tagagttggg cttcgccccc gcataacaac cataagcgcg agagccatga gccaagccga
     2461 tctgatttcc ccgctgcgtt tccttgcccg tctcccccag cacctgccgc gtgtgccgcg
     2521 catgttgcgc ggcctgtatt atgccggtat ccgcaatcgc gagaagaacc tgtcgctggc
     2581 ctgggcgctg gagcgcacgg cgcagcagca tcccgaccgt ccggcggtgc tggatgagtc
     2641 gcgcagcctt agttatgccc agttcaatgc cttggccaac cgcctggcct gggccttcaa
     2701 ggccgaagga gtgcgccatg gcaacgtggt agcggtgatg ctggagaatc gcctggagca
     2761 actggcgatc ctcgcgtcgc taagcaagct cggcgcggtc ggcgcgctgg tcaacaccac
     2821 ccagcgcggt aaggtgttgg cccacagctt taatctggtc aaacctggtt ttatggtgat
     2881 cggcgaagag ttgcgcgaag cgttcgagga agtggccgag caattggaca actccaacgc
     2941 accgcgctac tggattgccg atcaggattg tctggcggac gcaggcgttg cccccgacgg
     3001 ttggaccaat ctgatgcaac tggcgcgcag ccaggccagc gataacctgc cggacagcgc
     3061 caaagtgaag atgaaggacc cctgtttcct tatttacacc tccggtacca ccggcctgcc
     3121 caaagcctca atcatgagcc acggcaagtg gatcaaagcc tacggcggct tcggccattc
     3181 cggcctgacc ctgggcaatc aggacgtgct ctacctgacc ctgccctgct accacaacaa
     3241 cgcggtcacc gtgtgctgga gtgcagccat cgccggtggc gcggccatcg ccctgcgccg
     3301 caagttctcc gccagcgcct tctggccgga tgtgcagcgc tatcaggcca cttgcttcgg
     3361 ctatatcggc gaactgtgcc gctacctgct caaccagcct gaatgcgccg cagagaaaaa
     3421 caacagcctg cgttgcatga tcggcaacgg cctgcgcccc tcaatctggg ccgagttcaa
     3481 ggcgcgcttc ggcgttgagc agatcaccga gttctacgcc tccagcgaag gcaatatcgg
     3541 ctttaccaac gtattcaatt tcgacaatac cgtaggtttc tcccccgcca cctacgccat
     3601 cgtccgctac gacctggaga acgattgcgc catacgcacc gccaagggct ttctgcagaa
     3661 agccgacaag ggcgagcctg gcctgttgat cagcgagatc agcgccaaat ggccgttcga
     3721 cggttacacc gacccggcca agagcgaagc ggcgattctg cgcaacgtat tcaagaaggg
     3781 cgacgcctgg ttcaacaccg gcgacctgat gcgcgatatc ggctgcaagc acgcgcagtt
     3841 tgtcgaccgc ctcggcgaca ccttccgctg gaaaggcgag aacgtctcca ccaccgaggt
     3901 ggaaaacgta ctgggagcct tccccggcgt cgaggatgcg gtggtgtatg gcgtggaaat
     3961 ccccggcacc aacggccgct gcggcatggc cgcgctgcgt ctgagtgaag gccagcaact
     4021 ggatagcgcc gctctagccg cgcatctgga cgccgaattg ccggtgtatg ccgcgccgct
     4081 gtttatccgc ctgttgggtg aggtggaaac taccggcacc ttcaaataca agaagaccga
     4141 ccttaagcag gccgcctaca acccttcagc agtgcaggag ccgttgtggg tgcgcctgcc
     4201 aggcgagtcg cgctttaccc cgctggatcc cagcctgttc gcagccatcg gtgagcaggt
     4261 ctaccgcttc tgattgcaag cggcaggtag gctatcaacg ctgcctgcta ctgcttgaac
     4321 tcgaactgta actgcgcgcc ttccaccgat tgccagtagt cgtcatcgcg ctcgttggtg
     4381 atcagcttgc cattgagttg aaagggctcc tctgtcttat ccttgtcacc aaacataggc
     4441 ggaagtatcg ggatcgacag gtcgctgagc ggtgccaccg gctgcagggg atcaaacaca
     4501 ttggtattca gactcaaatc cagtggcgct ttcggctcag ctggctcgga cttgcttgca
     4561 accacagcag gttttggctt ggtttgagtc gctgcagcag gtttggcttc ggcagccttg
     4621 ggctgcgcag gcttgttcac gacgggcgcc ggctcggcct tagtcgcttt gctggtaacc
     4681 acgggttgag cgggttgcgc cttggcgatt tcaaccttgg gctttgcagg agggttggtg
     4741 ctttgctcgc aggccaccag tgacagcatt gtcagcagca gcgcgaggct tcgaataggc
     4801 aacatgagca ggtacaaagg cagtgacaca agtcgctatg ctggcccctc ccccccacgc
     4861 tggcaagctc gcaatcggta cggcaggtaa cagcgcaacc ttctattctg aggatcagcc
     4921 gacggccatc ggtgtcgatg gccgccagcg acttgcctta gcagccttat tcaggcgatt
     4981 gcagttgctg caactgcgcg tcgatatcca gctccggatg ctcgcgcatc agttgcaaca
     5041 gcaaagcatc ggcttcatcc gtccagccat cacggcgcag tttgagcaag cgttgcaact
     5101 cgctgtgcag ctgctgttca gcctggctgc ttgcggcttc tgccgctttt tcggaggctg
     5161 atacgtcagg cgcagcgtca tgcaatgcat cagccgcgga ctcttcacgt agcaactgac
     5221 tggacgcgcc ggccgaaagc ttcttctcgg ccttggcggc atcggcgctg tagccctgga
     5281 tctccgcacg cgaacgctgc gcctcttcgg caaccgcagc ggattctgcc aacggttcag
     5341 attgcggggc catcctcgcc gcagccggcg ccatgggtgc ggcgctcggc acggcatcaa
     5401 aggcatccgg agcctgctcg aaagtgcgcc aggtcaggct caccccgatc ccaaggcagg
     5461 caaccccggc caacgccacc gaccagcgtt gccgaccgcc agcgccaaac agccagctgt
     5521 gcaggcgctc gctcaaacca ggtttggctt cagcagcggg cgcgctacgc gctgctgcgc
     5581 tggccgccgc gagaatctgc gcatccagct gcgcactggg ctcaccgccg ctgtgctggc
     5641 ggaaatgcgc gagcatctgc cgctcctgct catcgagcct atgttgatca tggttcatac
     5701 ggacaactcc tcggcggccg ggtcagccag cagccgacgt aacttttgca gggcatagcg
     5761 caaacgactt ttcaccgtct cggcgggtat ctgggtcagc tcggcaatct cggccagttc
     5821 cagatcaccg tgtgcgcgca gcagaaacac ttcgcgctgc tctgccggta aatccgccag
     5881 cgcggcctct aatcgctgct gatcgcggct caggctgagc tgttgctcag gccccggctg
     5941 cgggtcgtgc ggggcatgct gctgttcatc gtactc
//
LOCUS       DASRUM010000263         6606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1477722, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000263 DASRUM010000000
VERSION     DASRUM010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477722"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..604
                     /gene="def"
                     /locus_tag="VFF22_17820"
     CDS             98..604
                     /gene="def"
                     /locus_tag="VFF22_17820"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZX18713.1"
                     /translation="MAILNILEFPDPRLRTIAKPVDVVDDALRQLIDDMFETMYDAPG
                     IGLAATQVNVHKRLVVMDLSEDKSEPRVFINPEFEPLTEEMDQYQEGCLSVPGFYENV
                     DRPQKVKIKALDRDGQPYELIAEGLLAVCIQHECDHLNGKLFVDYLSNLKRDRIKKKL
                     EKQHRQQA"
     gene            658..1602
                     /gene="fmt"
                     /locus_tag="VFF22_17825"
     CDS             658..1602
                     /gene="fmt"
                     /locus_tag="VFF22_17825"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZX18714.1"
                     /translation="MTEALRIVFAGTPEFAAEHLKALLDTPHQIIAVYSQPDRPAGRG
                     QKLTPSPVKQLALQHEIPVYQPQTLRDPAAQAELATLKPDLMVVVAYGLILPQVVLDT
                     PRLGCINSHASLLPRWRGAAPIQRAVEAGDAESGVTVMQMEAGLDTGPMLLKVSTPIS
                     AEDTGGSLHDRLATLGPQAVIAAISGLAAGNLVGEVQDDSLATYAHKLNKDEARLDWN
                     RPAIELERLVRAFHPWPICHSTLNSEALKVHAAELGEGADAPGTILAADKSGLTVACG
                     AGALRLTRLQLPGGKALNFSDLYNSRREQFAPGLVLGQ"
     gene            1599..2912
                     /gene="rsmB"
                     /locus_tag="VFF22_17830"
     CDS             1599..2912
                     /gene="rsmB"
                     /locus_tag="VFF22_17830"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016489962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HZX18715.1"
                     /translation="MNPRLAAARALAVVLNGKASLGSSLPPLLDKVEARDRGLTQDLA
                     FGTARWQPRLALIAEKLLQKPFKAADRDVEALLLIGLYQLFYTRIPAHAAIGETVACI
                     DKLKKPSLKGLLNAVLRNAQRDGESIIASLERDPVLHTAHPRWLQKALKAQWPEQWEA
                     ICAANNAHPPLILRVNRRLGSRDDYLAELRRVGFEAEPCSFSRDGIRLLQACDVTTLP
                     GFAEGRVSVQDEAAQLAAELLDLAPGQRVLDACAAPGGKTCHLLEAEPELAGVVAVDL
                     EEKRLVRVRENLKRLGLHAELIAADGRDLAAWWDGKPFQRILLDAPCSATGVIRRHPD
                     IKLTRQADDIPALAQLQGELLDSLWQTLEVGGILLYATCSVMPPENSENIAAFLARTP
                     GARELDIAGAFGIKPAHGRQLFPQVDGHDGFYYAKLMKIAAAPRG"
     gene            2925..4298
                     /gene="trkA"
                     /locus_tag="VFF22_17835"
     CDS             2925..4298
                     /gene="trkA"
                     /locus_tag="VFF22_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HZX18716.1"
                     /translation="MKIIILGAGQVGGTLAEHLASEANDITVVDTDGDRLRDLGDRLD
                     IRTVQGRGSFPTVLRQAGADDADMLVAVTNSDEVNMIACQVAYSLFHTPTKIARVREA
                     AYLTRSGLFCNESIPVDVLISPEQVVTNYIKRLIEYPGALQVIDFAEGKAQLVAVKAY
                     YGGPLVGQQLRQLREHMPNVDTRVAAIFRRNRAIMPQGDTVIEADDEVFFIAAKAHIR
                     AVMSEMRRMEDSYKKVVIAGGGHIGERLAEAIESRYQVKIIEMNPARCRYLSETLDST
                     IVLQGSASDRDLLVEENISDADIFLALTNDDEANIMSSLLAKRLGARKVMTIINNPAY
                     VDLVQGGEIDIAISPQLATIGTLLTHVRRGDIESVHSLRRGAAEAIEVIAHGDAKSSK
                     VVGRAIAEIALPPGTTIGAIIRNEEVLIAHDNTVIETGDHVILFLVDKKYIRDAERLF
                     QVGLTFF"
     gene            4308..4625
                     /locus_tag="VFF22_17840"
     CDS             4308..4625
                     /locus_tag="VFF22_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18717.1"
                     /translation="MSTEALEKMLAKGMDNALLRFGLGKGYLDAAEFAQAAEHLQRCV
                     EFDPQYSAAWKLLGQSLNKQGLSDEARAAWMQGLRVAEEKGDKQAGKEISVFLKRLNK
                     LAQ"
     gene            complement(4646..4918)
                     /locus_tag="VFF22_17845"
     CDS             complement(4646..4918)
                     /locus_tag="VFF22_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZX18718.1"
                     /translation="MSNSRKNVRTPLKVRLRIDHPVHGELLVTTRDISECGVYVLMDQ
                     AQNMLAMGEQVQGQVQGLPMEAPILTLEVVRVEALGVGLRFVQTED"
     gene            complement(4986..5873)
                     /locus_tag="VFF22_17850"
     CDS             complement(4986..5873)
                     /locus_tag="VFF22_17850"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase"
                     /protein_id="HZX18719.1"
                     /translation="MEKLKGALVVGFLRLFALLPWRAVQAVGAAIGWLMWKLPNSSRE
                     VVRINLAKCFPELDAAERERLVGRSLQDIGKTLTESACAWIWPAQKSIGLVREVEGLD
                     VLKEALASGKGVVGITSHLGNWEVLNHFYCAQCKPIIFYRPPKLKAVDDLLRQQRAQM
                     GNRVAPSTKEGILSVIKEVRKGGTVGIPADPEPSLSSGVFVSFLGTEALTSKFVAGML
                     SGGKACGVFLHALRLPDGSGFKVIFEAAPEDMYSTDTATAVAAMSRVIERYVRTYPSQ
                     YMWSMKRFKKRPAGEAKWY"
     gene            complement(5919..6476)
                     /locus_tag="VFF22_17855"
     CDS             complement(5919..6476)
                     /locus_tag="VFF22_17855"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017518859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HZX18720.1"
                     /translation="MPRCSWCSDDPLYQAYHDEEWGVPQRDPQVLFELLLLEAFQAGL
                     SWITVLKKRERYREVLFGFDVHRLAALTDEYIEETLMQDPGIIRNRLKLKAVRKNAQA
                     WLELEDPVTLLWSFVGGAPKINHFSERGQVPAVTPEAEAMSKALKKAGFTFVGPTICY
                     AYMQATGMVMDHTVDCDRYAQLTTR"
     gene            6543..>6606
                     /locus_tag="VFF22_17860"
     CDS             6543..>6606
                     /locus_tag="VFF22_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_064729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18721.1"
                     /translation="MSQTTPAVRTFQDLILALQQY"
BASE COUNT         1341 a         2116 c         1948 g         1201 t
ORIGIN      
        1 aacccagtcg tgctgcggcg tgaagccgtt tttcaatgct gctcatcatc ttagcagcgg
       61 attttgactt tattccacaa gtgcatcaca aacgcatatg gcgatcctga atatcctcga
      121 atttcccgat ccgcgcctgc gtaccatcgc caaaccggtg gacgtagtcg atgacgcctt
      181 gcgtcaactg atcgatgaca tgtttgaaac catgtacgac gcaccaggca tcggtctggc
      241 ggcgacgcag gtcaacgtgc acaaacgtct ggtggtaatg gacttgtccg aagacaagtc
      301 cgagccccgg gtgtttatca atcccgagtt cgaaccgctg accgaagaaa tggatcagta
      361 ccaggaaggc tgcttgtcag tgccgggttt ctacgaaaac gtcgaccgcc cgcagaaggt
      421 caagatcaag gcgctggacc gcgatggcca gccctatgag ttgatcgctg agggcctgct
      481 tgccgtttgc atccagcacg aatgcgacca ccttaacggc aagctgtttg tcgattacct
      541 gtctaatctc aaacgcgacc ggatcaagaa gaagctggaa aagcaacatc gccagcaggc
      601 ttgagccaca cctttcaaag gcttgctgcg gcaagccttt ttctttagcg agcccccatg
      661 actgaagcac tgcgtattgt ctttgccggc accccggaat tcgccgccga acacctcaag
      721 gccttgctcg acacgccaca ccagattatt gccgtctaca gccagcctga tcgcccggcc
      781 ggccgtggcc agaaactcac acccagcccg gtcaagcagc ttgcgctgca acacgaaatc
      841 ccggtgtatc agccgcagac cctgcgcgac cccgcggccc aagccgaact ggcgacgctc
      901 aagcccgacc tgatggtggt ggtggcttac ggcctgattc tgccgcaggt agtgctcgat
      961 accccgcgcc tgggctgcat caacagtcac gcctcgctgc tcccacgctg gcgtggcgcc
     1021 gcaccgatcc agcgcgcggt tgaggctggt gatgccgaat ccggcgtcac cgtgatgcag
     1081 atggaagccg gcctggatac cgggccgatg ttgctcaagg tcagcacgcc aatttctgct
     1141 gaagacaccg gcggtagcct gcacgatcgc ctggccacac tcggcccaca ggccgtgatc
     1201 gccgccatca gcggcctggc cgccggcaat ctggttggcg aagtgcagga cgacagcctg
     1261 gccacctacg cgcacaagct gaacaaggat gaagcgcgtc tcgactggaa ccgcccggcc
     1321 attgagctgg agcgactggt acgcgccttt cacccgtggc cgatctgtca tagcacgctc
     1381 aatagcgaag cactcaaagt gcatgcggcc gagctgggcg agggcgccga cgctcccggc
     1441 actattctcg ccgccgataa atctgggctg accgtggcct gcggcgcagg tgcgctgcgt
     1501 ctaacgcgcc tacagctacc cggcggcaag gcattgaatt tcagtgacct gtacaacagc
     1561 cgccgcgagc aattcgctcc aggcctggtg ctcggtcaat gaacccacgt ctcgccgccg
     1621 cccgcgccct ggccgtggtg ctcaatggca aagcctcact gggtagcagc ctgccgccgt
     1681 tgctggataa ggtcgaggcc cgcgaccgcg gcctcaccca ggatctggca tttggcaccg
     1741 cccgctggca gccgcgcctg gccttaattg cggagaagct gctgcaaaag cccttcaagg
     1801 ctgccgaccg cgatgtcgag gcgctgctgt tgatcggcct gtatcagctg ttctatacgc
     1861 gcatcccggc ccatgccgcc atcggcgaaa ccgtggcctg catcgacaag ctgaaaaagc
     1921 cctcactcaa ggggctactc aatgccgtgc tgcgcaatgc ccagcgcgac ggcgagagca
     1981 ttattgccag cctggagcgc gatccggtgc tacataccgc tcacccgcgc tggctgcaga
     2041 aagccctgaa agcccaatgg ccggaacagt gggaagccat ctgcgcggcc aacaacgccc
     2101 acccaccgct gattctgcgg gtcaaccgcc gcctcggcag ccgtgatgac tacctggccg
     2161 aactgcgccg cgtcggtttt gaagcagaac cctgcagctt tagccgcgac ggcatccgct
     2221 tgctgcaagc ctgtgatgtg accaccctgc cgggttttgc tgaaggccgc gtcagcgtgc
     2281 aggacgaagc ggcgcaactg gccgccgaac tgctggatct cgcccccggc caacgggtgc
     2341 tggacgcctg cgccgcgccc ggcggcaaaa cctgccactt gctggaagcc gagcccgagc
     2401 tggccggcgt tgtggcggtg gatctggaag aaaaacgtct ggtgcgggtg cgcgagaacc
     2461 tcaaacgcct gggcctacac gccgagctga tcgctgccga tggccgtgat ttggccgcct
     2521 ggtgggacgg caagccgttc cagcgcatcc tgctcgacgc cccctgctcg gccaccgggg
     2581 tgatccgccg gcacccggat atcaaactga cccgccaggc cgatgatatt cccgccctgg
     2641 cccagctgca gggcgaactg ctggacagcc tgtggcagac cctggaggtc ggcggcatcc
     2701 tgctgtatgc gacctgttcg gtgatgccgc cggagaacag cgagaatatt gctgccttcc
     2761 tggcgcgcac gccgggtgcc cgcgagctgg atatcgccgg ggcattcggt atcaagcctg
     2821 cccatgggcg ccagctattc ccgcaagtag acggccacga cggcttctac tatgccaagc
     2881 tgatgaaaat cgctgccgcc ccgcgcggct gaggaacaga ctgcatgaag atcatcattc
     2941 tcggtgcagg ccaggttggc ggcaccctgg cggagcacct ggccagcgaa gcgaacgaca
     3001 tcaccgtggt cgacaccgac ggtgatcgcc tgcgtgacct tggcgatcgt ctggatatcc
     3061 gcaccgtaca gggccgcggc tccttcccca cagtgctgcg tcaggccggc gcggacgatg
     3121 ccgacatgct ggtggcggtg accaacagcg atgaggtcaa catgatcgcc tgccaggtgg
     3181 cctacagcct gttccacacc ccgaccaaga ttgcccgagt gcgtgaagca gcctacctga
     3241 cccgcagcgg cctgttctgc aacgagtcga ttccagtcga tgtactgatc agccccgagc
     3301 aggtggtgac caactacatc aaacgcctga tcgaataccc cggtgccctg caggtaatcg
     3361 acttcgccga aggcaaggcc cagctggtgg cggtcaaggc ttactacggc ggcccgctgg
     3421 tcggtcagca actgcgccag ttgcgcgaac acatgcccaa tgtcgacacc cgggttgcgg
     3481 cgatcttccg ccgcaaccgg gcgatcatgc cccagggcga tacagtgatc gaagccgatg
     3541 acgaggtgtt ctttatcgcc gccaaggcgc atatacgtgc ggtgatgagc gaaatgcgcc
     3601 gcatggaaga cagctacaag aaggtggtga tcgccggcgg cgggcatatc ggcgagcgcc
     3661 tggccgaggc catcgagagc cgctaccagg tgaagatcat cgagatgaac ccggcgcgct
     3721 gccgctatct ctcggaaacc ctggatagca ccatcgtgct gcagggcagc gcctcggacc
     3781 gcgacctgct ggtggaagag aacatcagcg acgccgatat cttcctggcc ctgaccaacg
     3841 acgacgaggc caatatcatg tcgtccttgc tggccaaacg cctcggcgcg cgcaaggtga
     3901 tgaccatcat caacaacccg gcctatgtag acctggtgca gggcggcgaa atcgacatcg
     3961 ccatcagccc gcagctggcc accatcggca ccctgctgac ccacgtacgc cgtggcgata
     4021 tcgaaagcgt gcacagcctg cgccgtggcg cggccgaggc cattgaggtg attgcccatg
     4081 gtgatgccaa atcgagcaag gtagtcggcc gcgccatcgc cgaaatcgcc ctgccgccgg
     4141 gcaccaccat cggtgcgatc atccgcaacg aagaggtgtt gatcgcccat gacaacacgg
     4201 tgatcgagac gggcgaccat gtgatcctgt tcctggtcga taaaaaatat atccgcgatg
     4261 ccgagcgctt gttccaggtc ggcctgacat ttttctagga atcgcccatg agtactgaag
     4321 ccctggagaa gatgctggcc aagggaatgg acaacgccct gctgcgcttc ggcctgggca
     4381 agggctatct ggatgcggct gagttcgccc aggcggcgga gcatttgcag cgctgtgtcg
     4441 agttcgatcc gcagtattca gccgcctgga agctactcgg ccagtcgttg aacaaacagg
     4501 gacttagcga cgaggcgcga gctgcctgga tgcaaggctt gcgagtagca gaagagaagg
     4561 gtgacaagca ggcgggtaag gaaatcagtg tgtttcttaa gcgtctgaac aagctcgctc
     4621 agtaggccaa gtaagagcgc ttagactagt cctcggtttg cacaaagcgc aggccaacgc
     4681 ccagcgcttc aacgcgtacc acttcgagcg tgagaatcgg tgcttccatc ggcaaaccct
     4741 gcacctgacc ctgcacctgt tcgcccatgg cgagcatatt ttgcgcctgg tccatcagca
     4801 catagacgcc acattcagaa atatcccggg tggtgaccag cagctcgccg tgcactgggt
     4861 ggtcgatgcg caggcgtacc ttgaggggtg tgcggacatt ctttcgacta ttgctcatgc
     4921 gccgtccgtc agagtgagtt tccccagcct agtcgagagc aggctcagcc tgcaactcgg
     4981 acacattaat accacttggc ctcacctgca ggacgcttct tgaagcgctt catgctccac
     5041 atgtactggc tcgggtacgt acgcacataa cgctcgataa ccctgctcat cgccgcaacg
     5101 gcagttgccg tatcggtgct gtacatgtcc tccggcgcgg cctcaaagat gaccttgaac
     5161 ccggagccgt ccggcagacg cagggcatgc agaaatacgc cgcaagcctt gccgccactc
     5221 aacatgccgg caacgaactt gctggtcagc gcctcggtac ccagaaagga cacgaatacc
     5281 cccgaagaca ggctcggctc tgggtcggcg ggaataccca ccgtaccgcc tttgcgcact
     5341 tccttgatca cgctgaggat gccttccttg gtcgacggtg cgacgcgatt gcccatctgc
     5401 gcgcgctgtt ggcgtagcag atcgtccacc gccttgagct tcggtgggcg gtaaaagatg
     5461 atcggtttgc actgcgcgca atagaagtgg ttgagcactt cccaattacc caggtggctg
     5521 gtgataccga ccacgccctt accgctggcc aaggcttcct tgagcacgtc caggccttcg
     5581 acttcacgca ccaggccgat ggacttctgt gccggccaga tccaggcgca ggcgctttcg
     5641 gtcaaggtct tgccgatatc ctgaaggctg cgcccgacca aacgctcgcg ctcggccgcg
     5701 tccagctcag gaaagcattt ggccaggttg atacgcacca cctcgcggga actgttcggc
     5761 agcttccaca tcaaccagcc aatggctgcg cccacagcct gtacggcgcg ccacggcagc
     5821 aaggcaaaca aacgcagaaa gcccaccacc agggcacctt tgagcttttc cacaggcaac
     5881 tccagccgat caaaaggcgc ttattgtaac cacccgggct agcgagtggt cagttgcgcg
     5941 tagcggtcac aatcaacggt gtgatccatg accatgccgg tggcctgcat ataggcgtag
     6001 cagatggtcg ggccgacaaa ggtaaagccg gcctttttca gcgccttgct catcgcctcg
     6061 gcctcagggg taaccgccgg cacctggccg cgctcgctga agtgattgat cttcggcgcg
     6121 ccgccaacaa acgaccagag cagcgtcacc gggtcttcca gctcgagcca ggcctgcgcg
     6181 ttcttgcgca cggccttaag cttgaggcga ttgcggatga tgccggggtc ctgcatcagg
     6241 gtttcttcga tgtactcgtc ggtgagcgcc gccaaccgat gcacatcgaa gccgaacagc
     6301 acctcgcggt aacgctcgcg cttcttcagc acggtgatcc atgacaggcc ggcctggaag
     6361 gcttcgagca gcagcaactc gaacagcacc tgggggtcgc gttgcggcac gccccattct
     6421 tcatcgtgat aagcctggta caacgggtcg tcagagcacc aagagcagcg tggcatgcgg
     6481 gttttcctga gcttcggtat actcgcggac tttctgaagc cgccaaagta cgggtgaaat
     6541 ttgtgagcca gactacgcct gccgtgcgca ccttccaaga cttgatcctc gccctgcaac
     6601 agtact
//
LOCUS       DASRUM010000264         8140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_972965, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000264 DASRUM010000000
VERSION     DASRUM010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8140
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972965"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..100)
                     /locus_tag="VFF22_17865"
     CDS             complement(<1..100)
                     /locus_tag="VFF22_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015634003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HZX18722.1"
                     /translation="MKRLGALLLLATLTACQSQNPYVADSKPLPPAP"
     gene            complement(130..879)
                     /locus_tag="VFF22_17870"
     CDS             complement(130..879)
                     /locus_tag="VFF22_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZX18723.1"
                     /translation="MTDRHFDELATRFAEKIYGGAKGAIRLAVLQADLAETLPDRPLR
                     VLDIGAGLGHMSLWLAERGHQVTLTEPAEPMLNGARERFAAAGQQATFIQAPWQDLLG
                     QLEQPYDLVICHAVLEWLAEPHSILPVLHQLCMPGGWLSLAFYNKDALIYRNLLKGHF
                     RKLRKAEFAGEKQSLTPQMPLDPRELAAQLEANWQVESQSGVRVFHDYMPQPFQAKAE
                     LLDLLEMELAYRRHPSFAGLGRYLHWICRPR"
     gene            complement(876..1190)
                     /locus_tag="VFF22_17875"
     CDS             complement(876..1190)
                     /locus_tag="VFF22_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HZX18724.1"
                     /translation="MNLAELTTRMHAIRDRNDWRPFHSPKNLAMAASVEMAELVEIFQ
                     WLTEAQSRALSAEQLTHAGQEIGDIVLYLVLLCGELGLDMEQVVHAKLADNERRFLGD
                     VP"
     gene            complement(1283..2137)
                     /locus_tag="VFF22_17880"
     CDS             complement(1283..2137)
                     /locus_tag="VFF22_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HZX18725.1"
                     /translation="MAIDWLELPTPPALPALFLRAALRRSVTGRSLPELGVRCQVSVD
                     PKHLARYRQICGFADNNLLPAPYPHILAFALQMQLLTDKRFPFPLLGLVHLENHIRVL
                     RPLGGLGPFTVSVQVQDLQAHDKGATFSLITQLHDQLGLLWEGDSRILCRALRMDGKP
                     AEREAETVLEQTTLAHWRAPADIGRRYARIAGDYNPIHMFAVTAKLFGFPRAIAHGLW
                     NKGRALAELSARLPAAGYEVSVRFQKPVLLPAEVRLLASEPASSGQFSLLGGDELVHM
                     SGSWRPLD"
     gene            2319..3311
                     /locus_tag="VFF22_17885"
     CDS             2319..3311
                     /locus_tag="VFF22_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZX18726.1"
                     /translation="MRDLTDDVALMRPVIDALRASGADPDRVLARVGLPPGGLPTGRF
                     PHAAQALFWKAAIDECGEEDVGLYLAQHLPAFHGLLLEYLFLSSETFGEGLRHALRYV
                     RLLSDTLNAKLEVHGDRAVMVLGLIPGTPRHFPEMLAGGVIRLFDALTEGDFIPYEVQ
                     FMHTEGAPAERYQQVYGCPAQLGGERYALIFNASVLDKTSRHAAPELLRMHESLARRQ
                     LAEVERLDLVRNVRDLIGELLVDSGATLEQVAARLNMPARRLRERLAMAGVRFNDLVT
                     DYRCRLAKDLLLNTEERIEVIVERTGFSEPSTFYRAFKRWVGETPVEFRKRGKQ"
     gene            complement(3315..4085)
                     /locus_tag="VFF22_17890"
     CDS             complement(3315..4085)
                     /locus_tag="VFF22_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZX18727.1"
                     /translation="MPVSEILLLVLAGFAAGGMNALAGGGTFFSFPALLAAGLPPVTA
                     NATNAVALWPASLAGAWAARDSLRPLGRYLVPLLAAGLAGGLLGGLLLLIGGDAVFAK
                     LIPWLLLAATALFAASPWLSRWLAARRAEAAQPPHGPLSLAAHVGVSIYGGYFGAGMG
                     ILQLAAFSIEGHPLARANALKNLISAVIYSVATATFIIAGRVSWYELVILLVGATVGG
                     YVGGALGQRLPAKFLRLLVISVGSGMTLYYFWATYFAQ"
     gene            complement(4301..4828)
                     /gene="ppa"
                     /locus_tag="VFF22_17895"
     CDS             complement(4301..4828)
                     /gene="ppa"
                     /locus_tag="VFF22_17895"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HZX18728.1"
                     /translation="MSYSKIPAGKDLPNDIYVAIEIPANHAPIKYEIDHDSDCLMVDR
                     FMATPMFYPANYGFIPHTLADDGDPLDVLVVTPYPVAPGSVIRARPVGVLLMSDEAGG
                     DAKLVAVPHDKLSVLYQDVKEYTDLPPLLIEQIKHFFENYKDLEKGKWVKVEGWAGAD
                     EARNLINKAVAAYQK"
     gene            complement(4907..5746)
                     /locus_tag="VFF22_17900"
     CDS             complement(4907..5746)
                     /locus_tag="VFF22_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HZX18729.1"
                     /translation="MWSLRAWRRQRILARHPVSSEQWQAVLQQLPMLDGLSDAEQQRL
                     RERAVLFLHHKHLSALPGVKLDGHARLLLAVQAELPLLHLADLNWYQGFHEIVLYPDD
                     FVSPQRHRDANGVVHEWDAEHSGEAWLQGPVILAWPGVQASGQWDGYNLVIHELAHKL
                     DMLNGDANGLPPLHSNMAVQDWAQAMQSAFDQMNAELDANPEAETAIDPYAAEDPAEF
                     FAVTSEYFFSAPDLLAEAFPAVYAQLALFYRQDPLVRLQQLQASNPDYQVRTEDSEFA
                     TNG"
     gene            5828..7456
                     /locus_tag="VFF22_17905"
     CDS             5828..7456
                     /locus_tag="VFF22_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZX18730.1"
                     /translation="MLIRDLGGLPMSIERLHHPTVQLLCGHGLDLPSQAARARATWLP
                     GREGRPPVLLVALLWARECTDVVRTLDGYLDGLLADCCCTPSGWSEAQAARQVLAGFN
                     QQLFRQRQAGRNVVQLNAGLLLLQNGEAQFLQAGAIGLRRYQGDTATSLIGRDGLQLG
                     AQAELALVQQCLTLSPGELLLLAPQPLLDVADLQGFRGAGDALGSDPLAELLAPLLQA
                     PGAAVLLLPGEADSAPTLAPREPWRAVVNARPGLQLDGWELLSTCPYGPPGRVFRATD
                     RRGREAMLWLAEQDADEAFWQREWALRRSPVASLAQVMSSHQPREHAFWLFEPAGKGM
                     RSLIDWVAAHGPVDALTVLAVLEQLIAAVRGLQRRGMQGLWLAPRNILLDDGGRLLLL
                     PEYAALLPGVPPQKLPEQAMPLAPEARAGQAVDGRADQFALAALAYWLFCGRWPEVAQ
                     LDAGGNSRYVPLAQFSVQVPVGWDGVLARALAPRPEARFEALSEFQHALQQPLLHQPA
                     PTLRSRHYRQSWHLAALALLVLQLGVGLWLSLEG"
     gene            complement(7562..8029)
                     /locus_tag="VFF22_17910"
     CDS             complement(7562..8029)
                     /locus_tag="VFF22_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018925733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX18731.1"
                     /translation="MRIVQATLEHLDQLTPLFVNYREHFGQLPYPDSSRAFLEKRISR
                     KESVIYLAMADDEEKILGFCQLYSSFSSLSLKRVWILNDIYVCQDARRQLVADRLIQT
                     AKQMAKDTNAVRLRVASSINNEVAHKLYESIGFKEDTEFKNFVLPITEDFTAP"
BASE COUNT         1454 a         2411 c         2723 g         1552 t
ORIGIN      
        1 caggtgccgg cggcagcggt ttgctgtcgg cgacataagg attctggctc tgacaggccg
       61 tgagggtggc cagtagcagt aaggcgccaa gtcgtttcat cgcgttgcat gcctccagtg
      121 cgatggctgt cagcggggcc ggcagatcca gtgtaaataa cgtcccagcc cggcaaagct
      181 gggatggcgg cggtaggcca gctccatttc caacaggtcg agcagctcgg ccttggcttg
      241 gaagggttgc ggcatatagt cgtggaacac ccgcacgcca ctctggcttt cgacctgcca
      301 attagcctca agttgcgccg ccagctcgcg cggatcaagc ggcatctgcg gggtcaggct
      361 ctgtttctca ccggcgaact cggccttgcg cagtttgcgg aagtgaccct tgagcaggtt
      421 gcggtagatc agtgcgtcct tgttgtagaa cgccagcgac agccagccgc caggcataca
      481 cagctggtgc agcaccggca ggatgctgtg cggttcggcc agccattcca gcactgcatg
      541 gcagatcacc aggtcatagg gttgctcgag ctgaccgagc agatcctgcc agggcgcctg
      601 gataaaagtg gcttgttgtc cggcggcagc aaagcgctcg cgggcgccat tcagcatcgg
      661 ttcagcgggt tccgtcagcg tcacctgatg gccgcgctcg gccagccaca gcgacatatg
      721 gccaagcccc gcgccaatat ctagcacccg cagcggcctg tcgggcaggg tttcggccag
      781 atcggcctgg agtacggcca ggcgaatcgc gcccttggcg ccgccataga tcttctcggc
      841 gaagcgcgtg gccaactcgt caaagtgccg gtcggtcatg gcacgtctcc gagaaagcgc
      901 cgctcgttat cagccagctt ggcgtgcacc acctgttcca tatccaggcc cagctcgccg
      961 cacagcagca ccagatagag gacgatatcg ccgatttcct ggccggcatg ggtgagttgt
     1021 tcggcggata gcgcgcgtga ctgcgcctcg gtcagccact ggaagatttc caccagctcg
     1081 gccatttcca cgctggcggc catggccagg ttcttcgggc tgtgaaatgg ccgccagtcg
     1141 ttgcgatcac gaatggcgtg catgcgggtg gtcagttcgg cgaggttcat cgggcgctcc
     1201 agcaaagagc gcatagcttc gggggttcgc ctgcacggtg caagcggctg ccggatagtt
     1261 accggcagcc gtccgcttta tttcagtcga gcggtcgcca gctgccgctc atatgcacca
     1321 gctcatcccc gccaagcagg ctgaactggc cgctgctagc cggttcgctg gccagcaggc
     1381 gtacctcggc cggcagcagt accggtttct ggaagcgcac gctcacttca tagccggcag
     1441 caggcaggcg cgcgctcagt tcggcgaggg cacggccctt gttccacaga ccgtgggcga
     1501 tggcgcgggg gaagccgaac agcttggccg tgacggcaaa catatggatc gggttgtagt
     1561 cgccagcgat gcgcgcataa cgccggccga tatccgcggg cgctcgccag tgggccaggg
     1621 tggtctgttc aagcacggtt tcggcttcgc gttcggcagg tttgccgtcc atgcgcagag
     1681 cgcggcagag gatgcggctg tcgccttccc agagcaggcc gagctggtcg tgcagttgag
     1741 tgatcagact gaaggttgcg cctttgtcgt gggcttgcag gtcttgcacc tgcacgctga
     1801 cggtaaacgg tccgaggccg ccaagtgggc gcagcacacg gatatggttt tccagatgca
     1861 ccaggccgag cagcgggaaa gggaagcgtt tgtcggtgag cagctgcatc tgcagggcaa
     1921 acgccaggat gtgcgggtag ggcgcgggca gcaggttatt atcggcaaag ccgcagatct
     1981 gccgatagcg cgccagatgc ttgggatcaa ccgagacctg gcagcgcacg cctagctcgg
     2041 gtaggctgcg gccagtcacg ctgcggcgca gtgcggcacg tagaaataac gcgggtagag
     2101 cgggtggtgt gggcagttcg agccagtcga tggccatggg taaactcctc agatcggtgg
     2161 cagcagctta gtcggcccgc tcttatctgc attggccgcc atgcccgcaa gcacggcggg
     2221 ccagtcaatc gctcaaccgc gcacgattca cgccttgagc gcagggccta aagctgccta
     2281 agatgaccat gcctcgcaca caataagaac tccccatcat gcgtgacctg actgacgatg
     2341 tggcgctgat gcgcccggtg atcgacgcct tgcgtgccag cggtgccgac cctgaccgcg
     2401 tgctggcgcg tgtcggcctg ccgcctggtg gtttgcctac cgggcgtttc ccccatgcag
     2461 cccaggcctt gttctggaaa gccgccatcg atgagtgcgg cgaagaggat gttggcctct
     2521 acttggccca gcacctgccg gcattccacg gcctgctgct ggaatacctg ttcctttcca
     2581 gtgaaacctt cggcgaaggc ctgcgccatg ctctgcgcta tgtgcgcctg ctctccgata
     2641 ccctgaatgc caagctggag gtacatggcg atcgcgcggt aatggtgctt gggctgattc
     2701 ctgggacgcc acggcacttc ccggaaatgc tcgccggcgg ggtgatccgc ctgttcgatg
     2761 cgttaaccga aggcgatttc atcccctacg aagtgcaatt tatgcacacc gagggcgcgc
     2821 cggccgagcg ttatcagcag gtctacggtt gcccggcgca actgggcggc gaacgctatg
     2881 cgctgatctt caatgcctcg gtgctggata aaacttcacg gcatgccgcg ccggaactgc
     2941 tgcgcatgca tgagtccctg gcgcggcggc aactggctga agtcgaacgt ctggatctgg
     3001 tgcgcaatgt gcgcgacttg atcggcgagc tgctggtcga cagcggcgcc accctggagc
     3061 aggtcgccgc gcgcctgaac atgccggcgc ggcgtttgcg cgagcgtctg gccatggccg
     3121 gggtgcgctt caacgatctg gtcaccgact accgctgccg cctggccaag gacctgctgc
     3181 tcaacaccga agagcgcatt gaggtgatag tcgagcgcac cggcttctct gagccgagca
     3241 ccttctaccg cgccttcaag cgctgggttg gcgagacgcc ggtggagttt cgcaagcgcg
     3301 gcaagcagtg atgactactg ggcgaaatag gtcgcccaga agtaatagag cgtcatgccg
     3361 ctgcccacac tgatcaccag cagacgcagg aatttggcgg gcaaacgctg gcctagcgcg
     3421 ccgccgacat agccgcctac ggtggcgccc accagcagaa tcaccagctc ataccaactg
     3481 actcggccgg cgatgataaa agtcgcggtg gcgacgctgt agatcaccgc ggaaatcagg
     3541 ttcttcaggg cattggcgcg ggccagcggg tggccttcaa tggaaaacgc cgccagttgc
     3601 agaatgccca tgccggcgcc gaagtagccg ccatagatcg atacgccgac atgcgcggcc
     3661 agtgacagtg gaccgtgcgg cggttgtgct gcctcggcgc ggcgggcggc cagccagcgg
     3721 ctgagccagg gactggcggc aaacagcgcg gtggcggcca gcaacagcca gggaatcagc
     3781 ttggcaaaca ccgcatcgcc accgatcagc agcaacagcc cacccagcaa accaccggcc
     3841 agcccggcgg ccaacaatgg cactaaatag cgccccaacg gccgcagcga atcccgcgcc
     3901 gcccaggccc cggccaggct agccggccat agcgccacgg cattggtggc attggccgtc
     3961 accggcggca gacccgcagc gagcaacgcc ggaaaggaga aaaacgtgcc gccaccggcc
     4021 agggcattca tcccaccagc ggcaaaaccg gcaagcacca gcagcagaat ctcagacaca
     4081 ggcatggtaa aggctcgcgc aaaaggtcag cagcttaacc agcgcgtgcg gtggggccaa
     4141 gtcgaccaag gtcgttggcg catgggttgg ccaaatcgcg ggcataaaaa atggccgggg
     4201 ccatttttaa cgtcgcgtat cgacggctcg aagggtggct gcatggatgg cagacataaa
     4261 aaaaccggct tgtggccggt ttttaggggg tggtctcgac ttatttttgg taagccgcga
     4321 ctgccttatt aatcaggttg cgggcctcat cggcgccagc ccaaccttca accttgaccc
     4381 atttgccttt ctcgagatct ttgtagttct cgaagaagtg cttgatctgc tcgatcagca
     4441 gcgggggcag gtcggtgtat tccttaacgt cttggtacag cacgctcagt ttgtcgtgcg
     4501 gtacggcaac cagcttggca tcgccgccgg cttcgtcgct catcagcagt acgccaactg
     4561 gacgggcacg gataaccgag cctggcgcca ctgggtacgg ggtcacgacc agcacgtcga
     4621 gggggtcgcc gtcgtcagcc agggtgtgcg ggataaaacc gtagttggcc gggtagaaca
     4681 tcggggtggc catgaaacgg tcgaccatca ggcaatcgct gtcgtggtcg atttcgtatt
     4741 tgatcggcgc gtggttggcc gggatctcga tggcaacgta gatgtcgttc ggcaggtctt
     4801 taccggctgg gatcttgctg tagctcatgg ggcgactccc gaggggtggg ccaaaaaagt
     4861 ggcgcgatta taggcatatt cccccggcgt tgctacgccg ggatgcctag ccattggtcg
     4921 cgaactcact gtcttcggtt ctgacctgat agtccggatt gctcgcctgc aactgctgca
     4981 gacgcaccag cgggtcctgc cgatagaaca gtgccagctg cgcgtatacc gccgggaagg
     5041 cttcggcgag caggtccggg gcgctgaaga aatactcgct ggtgacggca aagaactccg
     5101 ccgggtcttc ggcggcgtaa gggtcgatgg cggtttcggc ctcggggttg gcatccagct
     5161 ctgcattcat ctgatcaaaa gcgctctgca tggcctgggc ccaatcctgc acggccatat
     5221 tgctgtgcag cggcggcagg ccgttggcgt cgccgttaag catatccagc ttgtgtgcca
     5281 gctcatggat caccaggttg tagccatccc attggccgct ggcctgcaca cccggccagg
     5341 cgagaatcac cggaccttgc agccaggctt cgccgctgtg ttcggcgtcc cattcgtgca
     5401 ccacgccgtt ggcgtcgcgg tggcgttgcg ggctgacgaa gtcatccggg tagaggacga
     5461 tttcatggaa gccctgatac cagttgaggt cggccaggtg cagcagcggc agttcggcct
     5521 gcaccgccag cagcaggcgt gcgtggccat ccagttttac gccgggcagg gcgctgaggt
     5581 gtttgtggtg cagaaacagc acggcgcgtt cgcgcaggcg ctgctgttcg gcgtcactca
     5641 ggccatcgag catcggcagc tgctgcaata ctgcctgcca ttgctcgcta ctgacgggat
     5701 gacgggcaag gatgcgctgg cgacgccagg cgcggagcga ccacatggat tctgctcgca
     5761 cggtggaaag cgcccaccat aagccgggtg tcagtgctgg gcaagtcggc acaaaggctg
     5821 caacagcatg cttatccgcg acctgggagg gctgccaatg tcgatcgagc gtctacatca
     5881 tcccaccgtg caattgcttt gcggtcatgg tctcgacctg cccagccagg ctgcacgggc
     5941 ccgcgcgacc tggctgccgg ggcgggaagg gcgaccgccg gtgctgctgg tggcgctgct
     6001 ctgggcgcgc gaatgcactg atgtagtgcg caccctcgac ggttatctgg atggcctgct
     6061 ggccgactgc tgctgcaccc caagcggctg gagcgaagcc caggcagcgc ggcaggtgct
     6121 ggcggggttc aatcagcagc tgtttcgtca gcgccaggcc gggcgcaacg tcgttcaact
     6181 gaatgccggc ttgttgctgc tgcagaacgg cgaggcgcag tttctccagg ccggcgccat
     6241 tggtttgcgg cgttatcaag gcgatacggc aaccagcctg attggtcgtg atggtctgca
     6301 gctgggcgca caggccgagc tggcgctggt gcagcaatgc ctgactctaa gtcccggcga
     6361 gctattgctg ctggcgccgc agccgctgct ggatgtagcg gatttgcagg gtttccgtgg
     6421 cgcaggcgat gcgctgggca gtgatccatt ggcagagctg cttgcgccat tgctgcaggc
     6481 ccctggcgca gcggtgttat tgctgccggg tgaagccgac agcgcgccga cgctggcccc
     6541 tcgtgagccc tggcgggcgg tggttaatgc gcgaccgggt ctgcagctcg atggttggga
     6601 gctgctctcg acctgcccct atggcccgcc tgggcgggtg ttccgcgcca ctgatcggcg
     6661 ggggcgtgag gccatgctgt ggctggccga acaggatgcg gatgaagcct tctggcagcg
     6721 cgagtgggcg ctgcggcgca gcccggtggc gagcctggcg caggtgatgt cttcgcatca
     6781 accgcgtgag catgcctttt ggctattcga gccagcgggc aagggcatgc gcagcctgat
     6841 cgattgggtg gctgcccatg gcccggtgga cgctctgacg gtgctggccg tgcttgaaca
     6901 attgattgcc gccgtacgtg gcctgcaacg gcgcggcatg caggggttgt ggctggcccc
     6961 gcgcaatatt ctgctggatg acggcggccg cctgctgttg ctgcctgaat atgccgcgtt
     7021 gctacccggc gtgccgccgc aaaaactgcc tgaacaggcg atgccgctgg caccagaggc
     7081 acgtgccggg caggctgtgg atggccgcgc cgaccagttt gccctggcgg cgctggccta
     7141 ctggttgttt tgtgggcgct ggcctgaggt ggcgcagttg gatgccggtg gcaatagccg
     7201 ttatgtgccg ttggcgcagt tcagtgtgca ggtgccggta ggctgggatg gtgtgctggc
     7261 gcgggccttg gcgcccaggc ctgaggcgcg ctttgaggcg ttgtcggagt ttcagcatgc
     7321 tttgcagcaa cccttgctgc atcagccagc gcctacgctg cgctcgcgcc actatcggca
     7381 gagctggcac ctggcggctc tggccttatt ggtactgcag ctgggtgtgg ggctgtggct
     7441 gagcttggaa ggctgaggac ggtgttgcgg ttgggagtag cgcagcgaag cccaacgaga
     7501 cataccaatg tctgttgggc ttcgctgcgc tcaacgccaa cctacttatc taaataaaga
     7561 cctacggcgc agtgaagtct tcggtgatcg gcaggacaaa gttcttgaac tcggtgtctt
     7621 ccttgaaacc gatggactcg tagagtttgt gggcgacctc gttgttgatg ctggaggcga
     7681 cgcgcaggcg cacggcgttg gtgtctttgg ccatctgctt ggcggtctgg atcaggcggt
     7741 cggccaccag ttggcggcgt gcgtcctggc agacgtagat atcgttgaga atccacacgc
     7801 gcttgagcga cagcgaggag aagctcgaat agagctggca gaagccgaga attttctctt
     7861 catcgtcggc catggccagg tagatcaccg attccttgcg gctgatgcgc ttttccagaa
     7921 aggcgcgcga ggagtcgggg tagggcagct ggccaaagtg ttcacggtaa ttgacgaaca
     7981 gtggcgtgag ttgatccaaa tgctccaggg tcgcttggac gatgcgcatg gtgagcctct
     8041 acttaaagtc atgtggatgg cttcggcaac acggcagcgc tgcctgcgaa cgaccccgat
     8101 gctgcctaaa gcttttggaa agtgcaatcc aaacaagcgt
//
LOCUS       DASRUM010000265        16475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1324144, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000265 DASRUM010000000
VERSION     DASRUM010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 16475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16475
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324144"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="VFF22_17915"
     CDS             <1..1102
                     /locus_tag="VFF22_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HZX18732.1"
                     /translation="TQLLSKELKADFVERTRLDYIEVRERTANRSTRTERLSYAKSVE
                     NKPKFDWATYQPTVPSFTGVKVLENIDLKVLAEYIDWTPFFISWDLAGKFPRILTDEV
                     VGEAATALYADAQEMLKKLIDEKLISARAVFGFWPANQVNDDDLEVYGDDGQPQATLH
                     HLRQQTIKPDGKPNFSLADFVAPKDSGITDYVGGFITTAGIGAEEVAKAYQDAGDDYN
                     SIMVKALADRLAEACAEWLHKEVRTNYWGYDPEEALSNDELIKEAYKGIRPAPGYPAC
                     PDHTEKKTLFTLLDPAAEFNKAGASGVFLTEHYAMFPAAAVSGWYFAHPQAQYFAVGK
                     IDKDQVESYTARKKQDLNVSERWLAPNLGYDN"
     gene            1213..2538
                     /locus_tag="VFF22_17920"
     CDS             1213..2538
                     /locus_tag="VFF22_17920"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat-containing protein kinase
                     family protein"
                     /protein_id="HZX18733.1"
                     /translation="MHSLKQLRSGELAGITRLDLCCDLTDFPEEIFSLADSLEVLNLS
                     GNRLSALPADLARLHKLRIVFCSNNLFTSVPEVLGNCTQLEMIGFKSNRIQHLPAAAL
                     PPALRWLILTDNQLQQLPAELGDCAHLQKLMLAGNQLRALPESLANLQRLELLRIAAN
                     RLEHLPEWLTKLPSLCWLAFAGNPLSDASETEILQQHPLPTIARDTLELGEILGQGAS
                     GIIRSATWQQPNQPAQAMAVKLFKGDVTSDGLAHSEMAACIAAGAHNNLISVAGPLQP
                     LQDAPPGLLLARIEPSFQALAGPPSLASCTRDCYAAQRRFTVAEALRILRGTAAAVVH
                     LHQRGILHGDLYAHNLLVDPAGHTLLGDFGAASFFDPQSNNGIALQQLEARAFACLLE
                     ELLVRCNKDDQPTQQTRLRHLHARCSHPQPGERPLFAEIQACLQACNVA"
     gene            complement(3023..6034)
                     /locus_tag="VFF22_17925"
     CDS             complement(3023..6034)
                     /locus_tag="VFF22_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HZX18734.1"
                     /translation="MTILEYLDSAEEKLRKCQLEAVRSFVRYAEENDTERGFLINLPT
                     GAGKTGVISLISHLSGALKVLVICHRKAVKDQLFREISRKFFRNTIDDQEFELKKTYR
                     DSNFDQGDGVYITSFQKLTMLGDEELKNVQGSFDLIIVDEGHSEPSPVWREIIRQSAA
                     MKVVITATPYRNDLFELNVSTSHFYVFTFKEAIEDGVIMEPQYEQVDGDDKLLAGILA
                     FLGANEDVKCIVKCKNLEEIKKYHDLISQSFTTASVHERLEINEAQNKFKRVGPALKV
                     EGLRVIIHQHKLDEGIDIPEAKLLILTYELGSGRELVQAVGRIVRSYGEVQPLVIDLS
                     RGANEGMWDGYQKFDDYLANGGASEFVSSLSTSYLIESFLESFPRYSYFDGKFKERVD
                     LNIIDPEDDLKIPHASICFIQKKNDFSLPLLMDRLYWELHGSGSLVKAYEEILDLQIM
                     IYVEFNSSRFFKNKIFFEPKLHVIIVKEIVSGVAVFDSGGGRYYNQEKYRLGDAIHID
                     KLTALAAKTAIHQIKETHARAIGQAVRRPESVALKGQNLDGGRGSQSNSRYALTMVKV
                     DNIGLDGKRDSSFYIGARSGRVVDQKESNFTLQDISQWIDAIGQCINVGGSAGRLIKS
                     YAQPITEKPTSEILSVLLDFTDLEAPIATAKGAVLPDFVYAEYQKGIAFDAQGDLIEL
                     ALNYSDESGNFEILLTGSSIGRPDIEWIVEYLNSGHRLKILYEDGVTYLAGAFYKLAL
                     PYERGIPAEDSFAGNALFPLDALKAPVLKEKGHTLDHKYHRTTRNNFDTDSIFHLIDL
                     LKGYGDPATPLGSLGPFTTYIPGCDIVLCCDMGVEPADFILSSPEKLCFVHVKCGDSL
                     NPESSAGAIAEVGSQAIKNIHMLVSGLKSVKPGNFSTWKQSWPAAGAEFPLDTRYRLV
                     EGTINHPAPPDDSLSEKVWDVICKRKISMKCKKEIWIVAGNSFSTKHFIRSMENPLAC
                     SPTSIQAYQLIEDWLSTADELDVDLKIFTSP"
     gene            complement(6303..7277)
                     /gene="cysK"
                     /locus_tag="VFF22_17930"
     CDS             complement(6303..7277)
                     /gene="cysK"
                     /locus_tag="VFF22_17930"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HZX18735.1"
                     /translation="MSRIFADNAQAIGNTPLVRINRLGPEGVTILAKIEGRNPAYSVK
                     CRIGASMVWDAEERGVLKPGMTIVEPTSGNTGIGLAFVAAARGYKLLLTMPASMSLER
                     RKVLKALGAELVLTEPAKGMKGAIEKAAEIAASDTATYFMPQQFNNPANPAIHEKTTG
                     PEIWNDTDGAIDVLVSGVGTGGTITGISRYIKKTQGKPILSVAVEPIGSPVITQTIAG
                     NEVKPSPNKIQGIGAGFVPGNLDLSMVDRVELVSDDDAKTVALRLMREEGILCGISCG
                     AAMAAALRIAAEPGMQGKTIVVILPDSGERYLSSMLFSDMFTEQELQQ"
     gene            7399..8475
                     /locus_tag="VFF22_17935"
     CDS             7399..8475
                     /locus_tag="VFF22_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003184220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HZX18736.1"
                     /translation="MKRRYSTPLWILLGLLGTLLVLHFALPVLVRDYLNDKLADMGDY
                     RGQITDVDLALWRGAYRINGLQIVKVDGQVPVPFVDAQSIDLSVSWHSLWYERAIVAR
                     VVFEQPELNFVDGGADPEASQTGQGVDWREQLNKLLPITLDEVRVSDGTLSFHNFNSS
                     PPVDIEANQINASLYNLTNVADEQGERVARFEGKALLLGHAPLEATAVFDPFKNFEDF
                     EFRLRSTDIELKRLNDFSSAYGKFDFNAGTGHVVIEATADDGQLSGYVKPLLRNVDVF
                     NWQQDVEVEDKGFFRSIWEALVGGGESVLRNQREDQFATQVELSGSVRQQDISAFQAF
                     LAILRNAFVQAFNTRFERDATEND"
     gene            8607..9452
                     /locus_tag="VFF22_17940"
     CDS             8607..9452
                     /locus_tag="VFF22_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZX18737.1"
                     /translation="MKFEGTQSYVATDDLKLAVNAAITLERPLLVKGEPGTGKTMLAE
                     QLAESFGARLITWHIKSTTKAHQGLYEYDAVSRLRDSQLDSDKVHDVRNYIKKGKLWE
                     AFEAEERVILLIDEIDKADIEFPNDLLQELDKMEFYVYETDETIKAKKRPIIIITSNN
                     EKELPDAFLRRCFFHYIAFPDRATLQKIVDVHYPNIKKDLVAEALDVFFDVRKVPGLK
                     KKPSTSELVDWLKLLMADNIGEAVLRERDPTKAIPPLAGALVKNEQDVQLLERLAFMS
                     RRASR"
     gene            9477..9872
                     /locus_tag="VFF22_17945"
     CDS             9477..9872
                     /locus_tag="VFF22_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZX18738.1"
                     /translation="MHSGSCLCAAVKYRIDAPIESATHCHCSMCRKAHGAAFATYGNV
                     RRKDFHFTQGGESLGIYQSSPGVTRTFCKHCGSTLQWYSEQPHPNWLSVALGTLDTPV
                     GEIAQKHIFPESKADWYAINDGLPLEWRS"
     gene            9908..11086
                     /locus_tag="VFF22_17950"
     CDS             9908..11086
                     /locus_tag="VFF22_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012722828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZX18739.1"
                     /translation="MLLNLFNEMRAAKVPVSVRELLDLINALKHNVVFADMDEFYFLS
                     RTILVKDERHFDKFDRAFGAYFKGLENLNQHIEALIPDEWLRKEFERSLTDEERAKIE
                     SLGGLDKLIEEFKKRLEEQKERHEGGNKWIGTGGTSPFGSGGFNPEGIRVGDAGKRQG
                     KAVKVWDQREYKNLDDQVELGTRNIKVALRRLRKFARQGAEDELDLGGTIDHTAKDGG
                     LLNIQMRPERRNAVKLLILFDIGGSMDAHVKVCEELFSACKTEFKHMEYFYFHNCVYE
                     SVWKNNLRRTSERFSTQDLLHKYGADYKVVFVGDAAMAPYEITQAGGSVEHWNEEPGY
                     VWIKRFMEKYKKLIWINPYPKDTWGYTASTNIMRELLNEQMYPLTLQGLEDGMRFLSK
                     "
     gene            complement(11130..13292)
                     /locus_tag="VFF22_17955"
     CDS             complement(11130..13292)
                     /locus_tag="VFF22_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017478518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX18740.1"
                     /translation="MKFKSLQMRICVTAGICLFFSSASLVGYGLFSGRANEAYVAEEV
                     SGLIEKATFREIENLAESRANAIQTKLQGALEAARTMANTFAASKAAQSPLDIGREQI
                     NTVLLSVLKNNPEFNGTYSCWEPDALDGQDFAFRNDQDGNNPQTGRFTPYWTRSADGT
                     IAVQPLVEYDSADRHPNGVLKGGWYIGPRDTLTESVLDPLPYVVQGKNVWLTTLSVPI
                     VANGKFYGVVGADFDISFIQKISEQMSAELYGGKGSVSILSHQGLVVADSQRSDLIGQ
                     AIKTLLPTSWEKVLGDIQAGRAQSLLDPATENFEVLMPIQLGRTGKPWALLIRLPKAV
                     VMSQAIALEQELQARSISNSVWQVSVGLVISLLALVALWLTAAKIVGPIREAAVLAGN
                     ISLGDFSQRLVQQTEDEVGQLSFALNDMSQSLQRQVRVAERISEGDLDLQVQLSSPKD
                     TLGISLEKMVGNLNGLISEVQRSATQITGSSAQVTELSQSLSDGASNSASSITEISAV
                     MTQMAAQTTDNAANAKKADLQSQASRTDAGESDKLMSELIAAMAEIDSSGKDITAIIT
                     TIDNIAAQTNLLALNAAIEAARAGELGRGFAVVADEVRSLAARSAEAAQQTSALIADS
                     SVKTQRGMIIAGRTAESLKSIVSGTTAVSNLVSQIYQASSEQASGLQQASIGLEQIDE
                     VTQKNQSNSQACAVAAKELSDRASLMQRELSRFKIKKA"
     gene            complement(14067..14957)
                     /locus_tag="VFF22_17960"
     CDS             complement(14067..14957)
                     /locus_tag="VFF22_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018928341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZX18741.1"
                     /translation="MHYDLIDLRLFVAIAEAKNLTRGAERVHLAASSASHRMRLLEAS
                     IGTPLLLREPRGVSLTRAGEALLRHARQVFAQLEQMHADLTPYAKGVRGHVSLWANTH
                     ATHTFLPDSLSTFLQRHPQVSISLEEHTSPHVLLAVARGEVEVGVVAGTIEGAEVELI
                     PYRADRLVLIAPVDHPLAAHASSLFAQVLDYPFVMLHSGSAIHTFTMNAAAALGRHLE
                     VRIQVRSFEAVCRMVSAGVGIGLVPRSAVTAGAQLAVIELEEPWAQRDLKVCVRKREL
                     LSGFAAQLVDCLTASSITSA"
     gene            15063..16025
                     /locus_tag="VFF22_17965"
     CDS             15063..16025
                     /locus_tag="VFF22_17965"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZX18742.1"
                     /translation="MPWKTWSAERLGIALAVLAAFGFSFKAIFVKLAYAAAPVDAITL
                     LTLRMSFALPIALWVTLWLCRAAPPLTRRDWGMLIALGLLGYYGASILDFVGLQYISA
                     ALERLILFIYPTMTVLIGVLFMGKRLEKRQIAALLLTYAGIGLAFAHDLQVAEDLHTV
                     LIGAAFVFGSALCYALYSSGAEVAIHRLGTIRFAALAIIVSTFATQIHFLATQPFSAL
                     QQPVEVYAYSAAMAIFSTVLPIFWQSAALRLIGSARTVLIGTLGPILTIVFAWLLLSE
                     PVSLAQLAGAGLVLAGVLLVSRRRTLPIQPIATEDAGVQVSKQA"
     gene            complement(16081..>16475)
                     /locus_tag="VFF22_17970"
     CDS             complement(16081..>16475)
                     /locus_tag="VFF22_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2937 family protein"
                     /protein_id="HZX18743.1"
                     /translation="GFRETAKRFFKGDLVALVGHYQASADPVMRSDAQSLGTLVERSA
                     FLERESKAMQGPWYGQVWHLATAADHELMEETFAAYRYQVLLAPEAIAWGIACAMLLA
                     WLLESLVLLVGMLFGAGRSQKVQQRHWR"
BASE COUNT         3787 a         4851 c         4331 g         3506 t
ORIGIN      
        1 cacccagttg ctgtccaaag aactcaaggc cgactttgtc gagcgcaccc gcctcgacta
       61 catcgaagtg cgcgagcgca ccgccaaccg cagcacccgc accgaacgcc tgagctacgc
      121 caaatcggtg gaaaacaaac ccaagttcga ctgggcgacg tatcagccca ccgtgccgag
      181 ctttaccggc gtgaaggtgc tggagaatat cgacctcaag gtgctggccg aatatatcga
      241 ctggaccccg ttctttatct cctgggacct ggccggcaag ttcccgcgca tcctcaccga
      301 tgaagtagtc ggcgaagccg ccactgcgct gtatgccgac gcccaggaaa tgctgaagaa
      361 gctgatcgac gagaaactga tcagtgcccg cgccgtattc ggcttctggc cagccaacca
      421 ggtgaatgac gacgaccttg aagtctatgg cgacgacggt cagccccaag ccaccctgca
      481 ccacctgcgc cagcaaacga tcaagccgga cggcaagccg aacttctccc tggccgactt
      541 tgttgcgccg aaagacagcg gcattaccga ttacgttggc ggctttatca ccaccgccgg
      601 catcggcgct gaggaagtgg ccaaggccta ccaggacgct ggcgacgact acaactcaat
      661 catggtcaag gccctggccg accgcctggc cgaagcctgc gccgaatggc tgcacaaaga
      721 agtgcgcacc aactactggg gctacgaccc agaagaagca ttgagcaacg acgagctgat
      781 caaggaagcc tacaaaggca tccgccctgc tcccggctac ccggcctgcc cggaccacac
      841 cgagaaaaag accctgttca ccctgctcga ccccgcagcc gaattcaaca aggccggcgc
      901 cagcggcgtg ttcctcaccg aacactacgc catgttcccg gcggcagcgg tgtccggctg
      961 gtacttcgcc cacccacagg cgcagtactt cgccgtcggc aagatcgaca aagaccaggt
     1021 ggaaagctac accgcacgca aaaagcagga cctcaacgtc agcgaacgct ggttggcgcc
     1081 taatctgggt tacgacaact aagcccacgg cagcgggcaa cagcccgctc agcccgcatc
     1141 cgccgtgcgg acacctttag ccagcccaat agcactgggg agtgttccca gtgcacgagt
     1201 tctagcgcct ttatgcacag ccttaaacaa cttcgcagcg gtgagctggc cggcattacc
     1261 cggctagacc tgtgctgcga cctgactgac ttcccagagg aaattttctc tctggccgac
     1321 agccttgaag tgcttaacct ctcgggcaat cgcctaagcg cactcccggc tgacttagcg
     1381 cgcctgcaca agctgcgtat tgtgttttgc tcaaacaacc tgttcacctc ggtacccgag
     1441 gtattgggca actgcacgca actcgaaatg atcggtttca agagcaaccg cattcagcat
     1501 ctgcccgctg cggcactgcc gcccgcactg cgctggctga tcctgaccga caatcaactc
     1561 cagcagctac cggctgagtt aggtgactgt gcacacctac aaaagctgat gctggcgggc
     1621 aaccagctcc gcgcgctacc cgaaagcctg gccaatctgc agcgcttgga gctgctgcgc
     1681 attgcggcta accgcctaga gcatctgccg gaatggctca cgaaattacc gtccctgtgc
     1741 tggctggcct ttgccggcaa tccactcagt gacgccagcg aaactgaaat tctccagcaa
     1801 catccactac caaccatcgc gcgcgacacg cttgagctgg gtgaaatact cggccagggt
     1861 gcctcgggga tcatccgcag tgcgacctgg cagcaaccca accagccggc gcaggccatg
     1921 gcggtcaaac tgttcaaagg tgatgtaacc agcgacgggc ttgcccacag tgagatggcc
     1981 gcctgtatag cagccggtgc acacaacaac ctgatcagcg ttgccggccc actgcaacca
     2041 ctgcaggacg ccccgcccgg cctgctactg gcgcgtattg aaccgagctt ccaagcactg
     2101 gccggaccgc cctcactcgc cagctgcacg cgcgactgct atgccgcgca gcgtcgtttc
     2161 accgtggcag aagccttacg cattttgcga ggcacagcgg cagccgtcgt tcacctgcat
     2221 caacgcggca tcctccacgg cgatttgtat gcccataacc tgctggtcga cccagccggg
     2281 cacaccctcc tcggcgattt cggcgcagcg tcgttttttg acccacagag caacaacggg
     2341 attgccctgc aacaacttga ggccagggcc tttgcctgct tgctagagga gttgttagtg
     2401 cgctgcaaca aagacgacca gcccactcaa caaacccgct tgaggcacct gcatgcgcgc
     2461 tgcagccacc cgcaaccagg cgagcgcccg ttgtttgccg agattcaagc atgcctgcaa
     2521 gcctgcaacg tggcctgaat aaatcatcag tgcttaaagg gcgttgaaaa ttaatgatct
     2581 agccgcctga cgactgcgaa ttgatcagaa ataaatcact tcgccctaat ttggttagac
     2641 ataacccgca cacaagcgat tggtgacagg cagaggtcga ccagaattgg ccaatcacaa
     2701 agagccgttc ccgatcagta acagacgctg aaaaaatggc accgtaggat ggcgttgagc
     2761 acagcgatac ccatcatcac caggtaagcc gagcagaaat accgccagta ttaaatggcc
     2821 tattcggctt aagggtgtca tcccatgatc cagacgatta cgtgatgggt atcgctgcgc
     2881 tcaacccatc ctacgtgctg aagtatccgc cgatcacaaa ctgccaccca tgcgtacacg
     2941 gtttaattca acaatgctca aaaggccccg caacttagag gcctactggc ttgaattttt
     3001 atgcacctaa gatgagctag gcttaagggg atgtaaaaat ttttaaatct acatcaagct
     3061 catccgcggt gctcagccag tcctcaatga gttgatatgc ctgaatacta gtgggcgaac
     3121 aggctagcgg gttttccatg cttctaatga agtgtttagt ggagaaggaa ttgcctgcaa
     3181 caatccaaat ctccttcttg catttcatcg atattttccg tttgcagata acatcccaga
     3241 ctttctcaga aagtgaatca tcaggtggcg caggatggtt aatagttcct tccaccaacc
     3301 tataacgggt gtctaacgga aactctgctc cagcagctgg ccaagattgc ttccatgtag
     3361 aaaaatttcc aggcttcacg cttttaaggc ctgaaactaa catgtggata ttttttattg
     3421 cctggctgcc aacttcagca atggcaccgg ctgaagattc tggatttaat gaatcaccac
     3481 atttaacatg aacgaagcac aacttctccg gcgaagataa aatgaaatca gcaggctcaa
     3541 cgcccatgtc acagcaaaga acgatatcac agccaggaat atatgttgta aatgggccaa
     3601 gcgatccaag tggagtcgct gggtctccat acccctttaa caggtcgatc agatgaaaaa
     3661 ttgaatctgt atcaaaatta tttctagtgg ttcgatggta cttatgatcg agggtgtgcc
     3721 ccttctcttt gaggacggga gctttaagtg catcaagcgg gaaaagggca ttacctgcga
     3781 agctgtcctc ggcaggaatt ccacgctcat agggtaaggc cagcttgtaa aaggctcctg
     3841 ccagatacgt tacgccgtct tcgtacagta tttttaatct gtgaccagaa ttcaaatact
     3901 cgacgatcca ttcgatatca ggtcttccta tggaagaccc agttagaaga atttcaaaat
     3961 ttccgctctc atccgaataa tttaacgcaa gttctataag atcaccttga gcgtcgaaag
     4021 caatcccttt ttgatattca gcataaacaa aatcaggtaa gactgcaccc ttagcagtcg
     4081 ctatcggcgc ctcaagatcc gtaaaatcca gtagcacgga caaaatttct gaagtgggtt
     4141 tttcggtgat aggctgcgca taggacttga tcaatcgccc agcacttcct ccgacattga
     4201 tacattggcc gatcgcatcg atccattgag aaatatcttg aagggtaaaa ttagattctt
     4261 tttggtcgac gacccgcccc gacctggcac caatgtaaaa gctgctgtct cttttgccgt
     4321 ctagaccaat attatcaacc ttcaccatag ttaatgcgta ccgggagttg ctctgactgc
     4381 cgcgcccgcc atcaaggttc tgccccttca gtgctacgga ctctggacga cgcacagctt
     4441 ggccgatagc ccgggcgtga gtttccttga tctgatgaat tgctgttttt gcggccaaag
     4501 cagtcaattt gtcaatatga attgcatcac caagtcgata tttttcctga ttgtaatacc
     4561 ggccaccacc gctgtcgaag acagccacac ccgaaacaat ttcttttaca ataatgacat
     4621 gcagctttgg ctcgaagaaa attttatttt taaaaaacct tgagctatta aactcgacat
     4681 aaatcataat ctgcaaatca agaatttctt cataagcttt gactaatgac ccagagccgt
     4741 gcaactccca atacagtcga tccataagta gtggcagaga gaaatcattt ttcttttgaa
     4801 taaagcatat cgacgcatga gggatcttga gatcatcttc tgggtcaatg atgttaagat
     4861 caactctctc tttgaattta ccgtcaaagt agctgtatct cggaaagctt tcgagaaaag
     4921 attcaatcag gtaacttgtg cttagtgagc taacaaattc tgatgcacca ccattggcga
     4981 gatagtcatc aaatttttga tagccgtccc acattccctc attcgcgccc cggcttagat
     5041 cgatcaccaa tggctggacc tctccatagc tacgtacgat ccgacctaca gcctgcacta
     5101 actcacgccc actccccaac tcatacgtga ggatcagtaa cttagcttct ggaatgtcaa
     5161 tcccttcgtc aagcttgtgc tggtgaatga taacccgcag tccttcgact ttaagagccg
     5221 gaccaactcg tttgaactta ttttgcgcct cgttgatttc tagtcgctca tgcacgcttg
     5281 ccgtggtgaa tgattgagaa ataaggtcat ggtatttttt tatctcctca agattcttgc
     5341 acttcactat gcacttcaca tcttcgttcg cgcctagaaa ggctaggatg cctgcaagta
     5401 atttatcgtc tccatcaact tgctcgtact ggggctccat aattacccca tcttcgatag
     5461 cctctttaaa tgtaaaaaca taaaaatgac tcgtgcttac attaagttca aacaagtcgt
     5521 tgcgataagg cgttgcggtg atcaccactt tcattgctgc ggactgacgt attatttccc
     5581 gccaaacagg agaaggttca gagtgccctt catccacaat aattagatca aaactgcctt
     5641 ggacgttttt tagttcttcg tcacctagca tcgtcagttt ctggaaactg gtgatataga
     5701 ctccgtcacc ctgatcaaaa ttagagtccc gataagtttt ctttagctca aactcttggt
     5761 catcaattgt attgcggaag aatttccttg agatttctcg aaacagctga tctttcacgg
     5821 cttttcgatg acatatcaca agaactttta aggcacctga tagatgtgat atgagagaga
     5881 taacccctgt tttcccggca cctgtgggta ggttgatcaa gaaacctctt tcagtatcgt
     5941 tttcttcggc atacctgacg aagctacgta cggcttccaa ctggcatttc cttaactttt
     6001 cttcagcaga atccaaatat tccaagatag tcacagcagt cttccatgca attaaagacg
     6061 ttcgtatcgg ctgttcaggc cacgatgcgg agacagagta tcaaaaggcc atcattccac
     6121 gcaccctggg gccaacggta ggtcttttat taccccacgc atgagagtag agcccccttc
     6181 tgatgaacaa ccatatccac cgtatctaag ccaatcaatc agcagcactg gctcgtaaat
     6241 cacctagtaa ccacccacaa aaaaaccgcc agaatttggc ggtttttttg tgggtgcaga
     6301 ctttactgct gcaactcctg ctcggtaaac atatcgctaa acaacatgct cgacaggtag
     6361 cgctcgccgg agtccggcag gatcaccacg atggttttac cctgcatgcc gggctcggcg
     6421 gcgatgcgca gcgcggcggc catggcggcg ccgcaggaga tgccgcagag aatgccttcc
     6481 tcgcgcatca aacgcagggc aaccgttttg gcatcgtcat cagaaaccag ctcaacccgg
     6541 tcgaccatgc tcaggtcgag gttgcctggg acaaaaccgg cgccgatgcc ctggatcttg
     6601 ttcgggctgg gtttgacttc attaccggcg atggtctggg tgatcaccgg cgagccaatc
     6661 ggctccactg ccaccgagag gatcggcttg ccctgggtct ttttgatata gcgcgaaata
     6721 ccggtgatgg tgccgccggt gccgacaccg gaaaccagca cgtcaatcgc gccatcggta
     6781 tcgttccaga tttccgggcc ggtggtcttt tcatggatcg cagggttggc cgggttgttg
     6841 aactgctgcg gcataaagta ggtagcggtg tcagaggcgg cgatttccgc tgccttctcg
     6901 atggcgccct tcatgccttt ggcaggttcg gtaagcacca gctcggcacc caaggccttg
     6961 agtaccttgc gccgctccag actcatcgaa gcgggcatgg tcagcagcaa cttgtaaccg
     7021 cgcgcagcag caacaaaggc caggccgatg ccggtattgc ccgaggtggg ttcgacaatg
     7081 gtcatgcccg gtttgagcac accgcgctct tcggcatccc agaccatgct cgcaccgatg
     7141 cggcacttca ccgagtaagc cgggttccgc ccttctatct tggccaggat ggtcacacct
     7201 tccggcccca ggcggttgat ccgaaccagc ggggtattac cgatggcttg tgcgttgtct
     7261 gcgaagatgc ggctcatggc gaggtccttg tgcgtgcgtt gccgaatgga ctatcaagcc
     7321 taagacccaa gaacctagca gtccagcgcg gcgatgcatt agctggaacc ccgtaacagc
     7381 tcacgagtca gtctatgtat gaaaagacgc tatagcactc ccctgtggat actcctcggc
     7441 ctgctcggca ccttactggt gctgcatttc gccttgcctg ttctggtgcg cgactatctg
     7501 aatgacaagc tggccgatat gggcgactac cgcggccaga tcactgatgt cgatctggcg
     7561 ctgtggcgtg gtgcctaccg catcaacggt ctgcaaattg tcaaagttga cggtcaagta
     7621 ccggtgcctt tcgtcgatgc gcaaagtatc gatctctcgg tgagctggca ttcgctgtgg
     7681 tacgaacggg ccatcgtcgc ccgcgtggta ttcgagcagc cggagctcaa tttcgtcgac
     7741 ggcggtgccg accctgaagc gtcgcaaacc ggtcaaggcg tggactggcg cgaacagttg
     7801 aacaaactgc tgccaatcac cctcgacgaa gtacgcgtga gcgacggcac cctgagcttt
     7861 cataacttca attcttcgcc acctgtcgac atcgaggcca accagatcaa tgccagcctc
     7921 tacaacctaa ccaatgtcgc cgacgaacaa ggcgagcgcg tcgcccgctt cgagggcaag
     7981 gcactgttgc tcggccatgc accgctggaa gccacggcgg tattcgaccc cttcaagaac
     8041 ttcgaagact tcgaatttcg cctgcgcagt accgatatcg aactcaagcg cctcaacgac
     8101 ttctccagcg cctatggcaa gttcgacttt aatgccggca ccggccatgt ggtgatcgaa
     8161 gccaccgccg acgacggcca actgagcggc tacgtgaaac ccctgttgcg caatgtcgat
     8221 gtattcaact ggcagcaaga cgtcgaagtc gaggacaagg gtttcttccg ctcgatctgg
     8281 gaagccctgg ttggcggcgg cgaaagcgtg ctgagaaacc agcgtgaaga ccaattcgcc
     8341 acccaggtcg aactgagcgg cagcgtgcgc cagcaggaca tcagcgcctt ccaggcgttt
     8401 ctggcgatcc tgcgcaacgc tttcgtccag gcgttcaata cgcgttttga gcgcgacgca
     8461 acggaaaacg actgaccagc aacagctcat tcagtgactg aatagcccga ctgcgttcac
     8521 aggccgatgc cgcgcacgtt atagttaccc cctttcacta agtgcatggc ctgggctacc
     8581 gggcgttacc gttcgaggat ttaccgatga agttcgaagg cacccagtcc tacgtcgcca
     8641 ccgacgacct caagctcgcg gtcaatgccg ccatcaccct ggagcgcccg ttgctggtca
     8701 agggtgagcc gggcaccggt aaaaccatgc tggccgaaca gctggccgaa tccttcggtg
     8761 cacgcctgat cacctggcac atcaaatcca ccaccaaggc ccaccagggc ctgtacgagt
     8821 acgacgcggt cagccgcctg cgcgactcgc agctggattc ggacaaagtc cacgatgtgc
     8881 gcaactacat caagaaaggc aagctgtggg aggccttcga ggccgaagag cgggtgattc
     8941 tgctgatcga cgagatcgac aaggccgaca tcgagttccc taacgacctg ctgcaagaac
     9001 tcgacaagat ggagttctac gtttacgaga ctgacgagac catcaaggcg aaaaaacgcc
     9061 cgatcatcat catcacctcg aacaacgaaa aagaactgcc ggacgccttc ctgcgccgct
     9121 gcttcttcca ttacatcgcc ttcccggatc gcgcgaccct gcagaagatc gttgatgtgc
     9181 actaccccaa catcaagaaa gatctggtcg ccgaagcgct ggacgtattc ttcgacgtgc
     9241 gcaaagtgcc gggcctgaag aagaagccat ccaccagcga actggtcgac tggctcaagc
     9301 tcttgatggc cgacaatatc ggcgaagccg tactgcgcga acgcgaccca accaaagcca
     9361 tcccgccact ggccggggca ttggtgaaga acgagcagga tgtgcagctg cttgagcgct
     9421 tggcctttat gagccgccgc gcttcgcgtt aggaatcggg agcgggaggc gaatccatgc
     9481 acagcggcag ttgcctttgc gctgcagtga aataccgcat cgatgcgcct atcgagtcgg
     9541 cgactcattg ccattgttcg atgtgccgca aagcccatgg cgcggcattc gccacttacg
     9601 gcaacgtacg gcgcaaggat ttccacttca ctcaaggcgg ggaatcactg ggcatctacc
     9661 agtcctcgcc gggcgtgacg cggacctttt gcaaacactg tggctcaacc ctgcagtggt
     9721 acagcgaaca gccccacccc aactggcttt cagttgcgct gggcacgctg gatacgccag
     9781 tgggtgaaat cgcgcagaag catatttttc cggaatcgaa ggccgactgg tacgccatca
     9841 atgatggcct gccattggag tggcgctctt aagcgtcgca ctcaaacgaa ttcacgaggg
     9901 cgcagccatg ctgctcaacc tgttcaatga aatgcgcgcc gccaaggtgc cggtgtcggt
     9961 gcgtgagctg ctcgacctga tcaacgcgtt gaagcacaac gtggtgttcg ccgatatgga
    10021 cgagttctat ttcctctcgc gcaccatcct ggtcaaggac gagcggcatt tcgacaagtt
    10081 cgaccgcgcg ttcggcgctt atttcaaggg tttggaaaac cttaaccaac atatcgaggc
    10141 actgatcccc gatgagtggc tgcgcaagga attcgagcgt tcgctgaccg atgaggaacg
    10201 cgccaagatc gaatccctgg gcggcctgga caagctgatc gaggagttca agaagcgcct
    10261 cgaagaacag aaagaacgcc acgaaggcgg caataagtgg atcggcacag gcggcaccag
    10321 cccgttcggc tccggcggct tcaacccgga aggcattcgc gtgggtgacg ccggcaagcg
    10381 ccagggcaag gcggtcaagg tctgggacca gcgcgaatac aagaacctcg acgatcaggt
    10441 cgaactgggc acgcgcaata tcaaggtggc cttgcgccgt ctgcgcaagt tcgcccgcca
    10501 aggtgccgag gacgagcttg atctcggcgg caccatcgac cacaccgcga aagacggcgg
    10561 cctgctgaat attcagatgc gcccggagcg gcgcaatgcg gtgaaactgc tgattctgtt
    10621 cgatatcggt ggctcgatgg acgcccacgt aaaggtctgc gaagaactgt tcagcgcctg
    10681 taaaaccgag ttcaagcata tggagtattt ctacttccat aactgcgtgt acgagtcggt
    10741 gtggaagaac aacctgcgcc gtacctccga gcgcttctcc acccaggatc tgctgcacaa
    10801 gtacggcgcg gactacaaag tggtgttcgt cggcgatgcg gctatggcgc cctacgaaat
    10861 cacccaggcc ggcggcagcg tcgagcactg gaacgaggag ccgggttacg tctggatcaa
    10921 gcgctttatg gagaagtaca agaagcttat ctggatcaac ccttacccca aggacacctg
    10981 gggttacacc gcctcgacca acatcatgcg tgaactgctc aacgagcaga tgtacccgct
    11041 gacgctgcaa ggcctggaag acggcatgcg ttttctgtcc aagtaacgca aacaggcccg
    11101 cgagggcctg ttgtgtaacg ccggcctggc taggccttct tgattttgaa gcggctcagc
    11161 tcccgctgca tcagcgaagc gcggtccgac aactccttgg ccgccaccgc acaggcttgt
    11221 gagttggact gatttttctg ggtcacttcg tcaatttgct cgagcccgat actcgcctgc
    11281 tgcaagccgg aagcctgttc gctggacgcc tggtagatct gtgacaccag gttggagacc
    11341 gcggtagtac cgctgacgat gcttttgagc gactcggcgg tgcgcccggc gatgatcatg
    11401 ccgcgctggg tcttgaccga agagtcagca atcagcgccg aggtttgctg cgccgcctcg
    11461 gcgctgcggg ctgccaggct gcgcacttca tcagcgacca cggcaaaacc gcgacccagc
    11521 tcaccggccc gtgcggcttc aatcgcagcg ttcaacgcca gcagattggt ctgggcggca
    11581 atattgtcga tggtggtgat aatcgcggtg atgtccttgc ccgagctgtc gatttcagcc
    11641 atggcagcaa tcagctcact catcagcttg tcgctttcac cggcatcggt gcgtgaggct
    11701 tgcgattgca agtcggcctt tttcgcattc gctgcgttgt cggtggtttg cgccgccatt
    11761 tgcgtcatta ccgcgctgat ctcggtaatc gatgaggccg agttggacgc cccgtccgac
    11821 agcgactggc tcagctcggt gacctgtgca gaactgccag tgatctgggt cgcgctgcgc
    11881 tgcacctcgg agatcaggcc attgaggttg ccgaccatct tctccaggga aatacccagg
    11941 gtgtccttag gtgacgatag ttggacctgc agatccagat ccccctcgga gatgcgctcg
    12001 gccacccgca cctgacgttg caggctttgc gacatgtcgt tgagggcaaa agacagctga
    12061 cccacttcat cctcggtttg ctgcaccaga cgctgggaga agtcgcccag gctgatattg
    12121 cccgccaata ccgccgcctc gcgaatcggc ccgacaattt ttgccgcggt cagccacagg
    12181 gcgaccagcg ccagcagaga aatcaccaaa cccacggaga cctgccagac gctgttgctg
    12241 atactgcggg cctgcagctc ttgctccagg gcaatcgcct ggctcataac tacggctttg
    12301 ggcaggcgaa tcaacagcgc ccaaggtttg ccggtgcgcc ccagctgaat cggcatcagc
    12361 acctcgaagt tctcggtggc agggtcgagc aagctctggg cgcgccccgc ctggatgtcc
    12421 cccagcacct tttcccagga cgtaggcagt aaggtcttga tcgcctgccc gatcaaatca
    12481 ctgcgctgac tgtcagccac caccagcccc tgatggctca agatcgatac gctgcccttg
    12541 ccgccataca gctcggcgga catctgctcg ctgattttct ggatgaagga aatatcgaag
    12601 tcagccccta caacgccgta gaacttacca ttggccacga tcggcaccga caaggtggtc
    12661 aaccagacat tcttgccctg caccacatag ggcagcgggt ctagcacgct ttctgtcagg
    12721 gtatcgcgcg ggccgatgta ccagccgccc ttcaaaaccc cgttggggtg gcgatccgcc
    12781 gagtcgtact ccaccagcgg ctgtaccgcg atagtgccgt cggcactgcg cgtccagtac
    12841 ggtgtaaagc gcccggtctg aggattattg ccatcctggt cgttgcgaaa cgcaaaatcc
    12901 tgcccatcca gggcatccgg ctcccagcag gaataggtgc cgttgaattc tgggttgttc
    12961 ttcagaacgc tgagcaatac cgtattgatt tgctcacgac caatgtccag tgggctttgc
    13021 gctgccttgc tggccgcaaa ggtgttggcc atggtgcgcg ccgcctccaa agccccctgc
    13081 aacttggttt gaatggcatt ggcgcgcgac tcggccaggt tttcaatttc ccgaaaggtc
    13141 gctttttcaa tcagaccaga aacttcttcg gcaacatacg cctcgtttgc ccggccgcta
    13201 aataggccat aacccaccag gctggcggac gagaaaaaca gacatatgcc tgctgtcaca
    13261 catatgcgca tctgcaacga cttgaacttc ataacgccct cggtctttct aattttgcgt
    13321 aaaagatggc agcatccaat tcccttgcac aaaacatcca aacaacgaaa accaacgcct
    13381 atgcattacg ctccaatgca aagtgagtgg gtgccacaag ggcgagagag tttatttggg
    13441 cagcacaacg cacgcagcac agtgtaaaaa cgcttcaccg cgcatttttt aaattgaagc
    13501 cagatggatc aacccggaaa agccgagggt tatattctta tttttactga acttatgtag
    13561 tagttggtta ttacgttaaa gctctctatt accgggatct ctaagcgccg caaaacattc
    13621 actggctcag cgcttgcgct gcttgagctt cgctggccca gcaactcaac gagtgcaggc
    13681 tgcctctgca acacacctgt tatttgacgc ccacccataa aataaagacc aagccaaaca
    13741 gctagtaacc actatgcacg acagatacat attaaatatt gacgcacatc aggactagaa
    13801 ccgattaaac agtaatccag aaatttaatt ccatcacttt agtcgtagtc cggatgacga
    13861 tacccataag ccaacagaaa gcacatagcg ctaaaacacc gaactgtaaa ggcaagccat
    13921 tgattaaaat aagccatgac acacacggcg ctttaaatac ctacgcagaa ctttcacttc
    13981 gcgccagtcg atatcacgcc tgaaggcgag cgcggcatcg taaccggcgg tataagtccc
    14041 tgcgtgaaaa accgtacaag gtggttttat gccgacgtga tactgctggc cgtcaggcaa
    14101 tcgaccagct gcgccgcaaa acccgacaac aattcacgct tgcgcacaca gaccttcaaa
    14161 tcacgctgcg cccagggttc ttccagctca atcaccgcca actgcgcgcc agcggttacc
    14221 gcactgcgcg gcaccaggcc gatgccaacg cctgcactga ccatgcggca caccgcctca
    14281 aagctgcgca cctgaatccg cacctcaagg tgtcgcccca gcgcggcggc ggcattcatg
    14341 gtgaaggtgt gaatggccga gcctgaatgc agcataacaa aggggtaatc cagcacctgg
    14401 gcaaataggc tgctggcgtg cgcggccagt ggatgatcga ccggggcaat caataccaac
    14461 cggtcggccc ggtatgggat cagttcaacc tcagcgcctt caatcgtccc cgccaccacg
    14521 ccaacttcca cctcgccgcg cgccaccgcc agcagcacgt gcgggctggt gtgctcctca
    14581 agcgaaatac tgacttgcgg atgacgctgc aaaaaggtcg agaggctgtc gggcaggaag
    14641 gtatgggtgg cgtgggtatt ggcccacaga ctgacatggc cgcgcacgcc tttggcgtag
    14701 ggggtaaggt cggcgtgcat ctgttcgagt tgggcaaaca cctgccgcgc atggcgtagc
    14761 aatgcctcgc cagcacgggt aaggctgacg ccgcgcggct cacgcagcaa tagcggcgtg
    14821 ccaatcgacg cttccagcag gcgcatgcgg tgactggccg aagacgccgc cagatgcacc
    14881 cgctcggcac cccgagtgag gttcttcgcc tcggcaatcg cgacaaacag gcgcagatca
    14941 atcaggtcat agtgcatagc gttagttaaa tccgaacgat cacttatgga aaagccaatt
    15001 ccatcgcgat tctataccca gcatcattcg gcgcagcaca actctttagg gactcactca
    15061 acatgccatg gaaaacctgg tcagccgaac ggttgggcat cgcacttgcg gtgctcgccg
    15121 ccttcggttt ctcgttcaag gcgatcttcg tcaagctggc ctacgccgcc gcgccagtgg
    15181 acgcgatcac attgctgacg ctgcgtatga gctttgccct gcctatcgca ctgtgggtca
    15241 cactgtggtt atgccgcgct gcgccgccgc tgactcgcag agactggggc atgctgatcg
    15301 ccctgggcct gctgggttac tacggggcca gcattctcga ttttgttggc ctgcaataca
    15361 tttcggcggc gctggagcgg ctgatcctgt ttatctaccc gaccatgacc gtgctgatcg
    15421 gcgtgctgtt tatgggcaag cgcctggaga agcgccagat tgcggcgctg ctgctgacct
    15481 acgccggtat cggcctggcg ttcgcccacg atctgcaggt ggcggaggat ctgcatacgg
    15541 tgctgatcgg tgctgccttc gtcttcggtt ctgcgctgtg ctacgcgctc tatagctccg
    15601 gggccgaggt ggcgattcac cggctcggca ccatccgctt tgctgccctg gcgattatcg
    15661 tgtccacctt cgccacgcag attcacttcc tcgctactca accgttttct gcactgcagc
    15721 agcctgtgga ggtgtatgcg tacagcgcgg cgatggcgat tttctccacg gtgctgccga
    15781 tattctggca atcggctgca ctgcgcctga ttggctcggc gcgcaccgtg ctgattggca
    15841 cactgggacc gatcctgacg attgtcttcg cctggttgct gttgtcggag ccggtatcgt
    15901 tggcccagct ggcaggtgcc ggactggttc tggcgggggt gttgctagtg tcgaggcgta
    15961 gaaccttgcc gattcagccg attgctaccg aggacgccgg cgttcaagtc agcaagcagg
    16021 cttaagaagc gaatcggcag aaaacaccaa gggctcttgc gagcccttgt gtaattcaac
    16081 ttagcgccaa tgccgctgct gcaccttctg gctgcggccc gcgccaaaca gcatgccgac
    16141 cagcagcacc aggctttcca gcagccaggc cagcagcatg gcgcaggcaa tgccccaggc
    16201 gatggcttcg ggggcgagca gcacttggta gcggtaggcg gcgaaggttt cttccatcag
    16261 ctcatgatct gccgctgtgg ccagatgcca gacctggcca taccaaggcc cttgcatggc
    16321 tttggattcg cgttcgagga aggccgagcg ctcgaccagg gtgcccaaac tctgcgcatc
    16381 gctgcgcatc acggggtcag cgctggcttg gtaatggcca accaacgcga ccagatcccc
    16441 cttgaaaaaa cgctttgcag tctcccgaaa gcctt
//
LOCUS       DASRUM010000266        11132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2462149, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000266 DASRUM010000000
VERSION     DASRUM010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 11132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11132
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2462149"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFF22_17975"
     CDS             <1..627
                     /locus_tag="VFF22_17975"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HZX18744.1"
                     /translation="IEGAFQGGSLGEVGGAKIAGALELAAEDNRNGTPTRAVLLLETG
                     GVRLQEANLGLAAIAEIQAAIVELRQYQPVIALIAGPVGCFGGMSIAAGLCSYLLVTR
                     EARLGLNGPQVIEQEAGLDEYDSRDRPFIWSLTGGEQRHASGLVDGYVSDDVEAIRGE
                     LHSLFAQGKPETERSRRHAWFLERLRAVDTSVQADAAAVRSAYQGAQA"
     gene            624..1430
                     /gene="mdcE"
                     /locus_tag="VFF22_17980"
     CDS             624..1430
                     /gene="mdcE"
                     /locus_tag="VFF22_17980"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit gamma"
                     /protein_id="HZX18745.1"
                     /translation="MTTAQEQRGLHWFQALAGTPQAPSGLPASVQVADGELAGQAVRY
                     IAVVPDAANPFPRARSGEVGLLEGWGLAQAVDAAIEADRDRPHKRALIAIVDVPSQAY
                     GRREEAYGIHQALAGAVDAYARARLAGHAVVGLLVGKAMSGAFLAHGYQANRLIALRD
                     SGVMVHAMGKAAAARITLRSVEELEALAAEVPPMAYDLDNYASLGLLADILDVQQVAQ
                     PTASDLAHVQDALSRALADIASSPRDLRNRLGAANRAASSQVRELLRAQW"
     gene            1519..2154
                     /locus_tag="VFF22_17985"
     CDS             1519..2154
                     /locus_tag="VFF22_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HZX18746.1"
                     /translation="MHPNPRPHDLLWGLRPEHLPQEAPAWARVALGGHVPVVVRRALA
                     EPGWVAVGVRGAAREQRYAGWMRMADISRLVSPEAVARAGRWRCYPQSHWPALRALSQ
                     LAPQLAELGLAWGVTGSLGFELASGVSAAHLDSDLDLLLRAPSPLPREWARGLCELLD
                     KAPGCIDVQLETPQGAVALREWASGAPRVLLKTQAGPLLLSDPWQPRQVAA"
     gene            2151..3086
                     /gene="mdcH"
                     /locus_tag="VFF22_17990"
     CDS             2151..3086
                     /gene="mdcH"
                     /locus_tag="VFF22_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit epsilon"
                     /protein_id="HZX18747.1"
                     /translation="MSTLWAFPGQGAQQPGMLHDLPDSIIVRACLDEASEVLGESVLA
                     LDTAEALRATRAVQLCLLIAGVASARLLQGRDAQPDYVAGLSIGAYAAAVVAGAVTFA
                     DALRLVALRGELMQQAYPEGYGMSAVLGLDLAHLEALLAEVNSPQLPVYLANINADNQ
                     LIIAGSHAAMACVAERARRLGAGMVKPLAVSVPSHCALLAEPAARLIEAFEAVSVQRP
                     SVRYLSGSSARLISDPEKLRDDLAHNMCRVVDWQGTLQTAFERGVRLHLELPPGNVLS
                     GLARRVFEPGMAIAFQGSRLDTLDAMLRQEVNRSR"
     gene            3149..3550
                     /gene="madL"
                     /locus_tag="VFF22_17995"
     CDS             3149..3550
                     /gene="madL"
                     /locus_tag="VFF22_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005342 - organic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015849 - organic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate transporter subunit MadL"
                     /protein_id="HZX18748.1"
                     /translation="MIIYGVAFLAFCTLAGIFIGELLGKMIGVPANVGGVGIAMMLLI
                     FLGSYLHKRGILDATSEKGVAFWSAIYIPIVVAMAAQQNVYGALSGGPMAILAGTAAV
                     VVAFALVPVLSRIGQKKPEESATAPLTKAPR"
     gene            3556..4320
                     /gene="madM"
                     /locus_tag="VFF22_18000"
     CDS             3556..4320
                     /gene="madM"
                     /locus_tag="VFF22_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005342 - organic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015849 - organic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate transporter subunit MadM"
                     /protein_id="HZX18749.1"
                     /translation="MYESLMKVLSGYGLLSGFAIIGLTMWVSYWISDTFTKGRLHGSA
                     IAILLGLLLSYIGGAFTGGTKGLVDIPLFAGVGLLGGAMLRDFAIVATGFGVSVEELK
                     RAGFAGVLALFVGVGASFVAGVGVAMAFGYTDAVSLTTIGTGAVTYIVGPVTGAAIGA
                     SSEVMALSIAAGLVKAILVMVATPFIAPYIGLNNPRSAVIFGGLMGTSSGVAGGLAAT
                     DPKLVPYGCLTAAFYTALGCLLGPSLLYFLMRGLFG"
     gene            complement(4436..5347)
                     /locus_tag="VFF22_18005"
     CDS             complement(4436..5347)
                     /locus_tag="VFF22_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019580637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZX18750.1"
                     /translation="MLIDEELTLKKLEIFLAFMRSGNLTRAAVELNTSNVSVHRAIHS
                     LESALRCPLFKHEGRNLIPLESAYVLEERAQKLVQDVVESVRMTREAAGFSAERFKLG
                     ALYSLTVKTVPQLIMGLKLRRSELNIDLILGSNVDLLYKLKNVELDAILVALNDSVSD
                     PDCEQLPLFADDIFLAVPIDSPFAMQAEVDLADLRDSTFITLTQGFATHQDGVRVFQQ
                     AGFEPKVAMQVNDIFTLLSMVSSGVGYALLPGRIAAVYENRVRLIPLQAKYHLQQHIG
                     VVFLKAKERDPNLLALLAECRMYSRQS"
     gene            complement(5423..5722)
                     /locus_tag="VFF22_18010"
     CDS             complement(5423..5722)
                     /locus_tag="VFF22_18010"
                     /inference="COORDINATES: protein motif:HMM:NF023148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="I78 family peptidase inhibitor"
                     /protein_id="HZX18751.1"
                     /translation="MALRHHLASLTLLALLVGCSSQQPTQPAAEITGDCNVDNMSDVL
                     GQNASAEMIKQAQQQANAKFLRVLAPGDAATMDYNPQRLNIDIDESEVIIRLTCG"
     gene            5884..6813
                     /locus_tag="VFF22_18015"
     CDS             5884..6813
                     /locus_tag="VFF22_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX18752.1"
                     /translation="MQTVISIQQLSKTYASGHPALQDINLDIRPGEIFALLGPNGAGK
                     TTLISIICGIVNPGGGRVLVGGKDIIKDYRASRSQIGLVPQELVSDVFETVWATVKFS
                     RGLFGKKPNPAYLEQLLKDLSLWDKRDAKIFELSGGMKRRVMIAKALSHEPQILFLDE
                     PTAGVDVELRRDMWNMVRRLRERGVTIILTTHYIEEAEEMADRIGVISKGRIILVEDK
                     QALMHKLGKKQLTLQLQQPLASIPAELARYPLELIDEGHALVFTFDTQSEHTGIAELL
                     KDLAQHGIDFKDLQSSQSSLEEIFVSLVKESRA"
     gene            6810..7571
                     /locus_tag="VFF22_18020"
                     /pseudo
     CDS             6810..7571
                     /locus_tag="VFF22_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714397.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            7657..8892
                     /locus_tag="VFF22_18025"
     CDS             7657..8892
                     /locus_tag="VFF22_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007925150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZX18753.1"
                     /translation="MDHSAALSALLAQLYRDESRRVLATLIRLLGDFDLAEEALHEAF
                     RAAMEQWPQTGVPANPRAWLVSAGRFKAIDNLRRQRRFQPLDEHVELVDDAPLGEGEL
                     LEDDRLRLIFTCCHPALASDAQVALTLREVCDLGTEEIARAFLTTPATLAQRIVRAKA
                     KIRDARIPYEVPGRSELPERLDAVLRVIYLVFNEGYFASSGDSLTRAHLSDEAIRLAR
                     LLLELLPEPEVQGLLALMLLHESRRSARSSANGEVVLLEAQDRRRWDRALIAEGDALV
                     LQALHSRRFGPYTLQAAIAAVHAEAAHAEQTDWAQIVGLYDALLRLNPSPVIELNRAV
                     AVAMQQGPEAGLALIDALLASGELADYHLAHAARADLNRRLGRVAPARDAYERALELA
                     QQGADRQFLQMRLDELPTG"
     gene            9027..9371
                     /locus_tag="VFF22_18030"
     CDS             9027..9371
                     /locus_tag="VFF22_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZX18754.1"
                     /translation="MKYLCLVYSNEHELHNSPDSPRDEECFAYAESVAESGRMLAAEA
                     LESVQTATTVRMRGGKLSIIDGPFAETKEQLAGFYLVEARDLNEAIQLAGHIPAARVG
                     CVEVRPVRELQV"
     gene            9420..10205
                     /locus_tag="VFF22_18035"
     CDS             9420..10205
                     /locus_tag="VFF22_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HZX18755.1"
                     /translation="MSRPEYPKIASREQWLIARKALLAKEKALTEQRDALNVERRQLP
                     MVKVEADYRFQGPEGEVGLVDLFAGRSQLIVYHFMYHMDRGEGCDGCSCVADNIGHLA
                     HLHARDTTLVMVSRAPLADLQAFQRRMGWTVPWYSSLGSAFNYDYHATTDEQVAPVEY
                     NYRDKAELERLGLKYHVQGEQPGVSVFLRDGAQVYHCYSSYGRGLEMLMSSFHYLDLT
                     PFGRGEGWGGMPDLEGKGLHWTRLHDEYQQPAAAHDCCASIKP"
     gene            10223..10651
                     /locus_tag="VFF22_18040"
     CDS             10223..10651
                     /locus_tag="VFF22_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018926431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZX18756.1"
                     /translation="MSNAQAQIQQQLDSWAAACRSKDVSQIMRHYAEDVVAYDAIGPL
                     RFQGRAAYQAHWQACMEMCSGQAMFEIHQPSFLLSDGLAVVHYLFHCGGTDDKGEMQS
                     SWMRVSQCFRQQGGQWLIAHEHFSMPCDMESGKVLFDLQP"
     gene            10824..>11132
                     /locus_tag="VFF22_18045"
     CDS             10824..>11132
                     /locus_tag="VFF22_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HZX18757.1"
                     /translation="MQIQVHSDNHIEGSARLVEWVSASVTSKLERFDDELTRIVVHLH
                     DDNGAKAGAHDKRCQIEARPKGLQPISVTHKAESLEQAIDGSIEKLHHALEHQFGKLR
                     S"
BASE COUNT         1944 a         3348 c         3600 g         2240 t
ORIGIN      
        1 atcgaagggg catttcaggg cggcagcctg ggtgaagtgg gcggggcgaa gattgccggt
       61 gctctggagc tggcggccga agacaaccgc aacggcacgc cgacccgcgc tgtattgctc
      121 ctggaaaccg gtggcgtgcg cctgcaggag gccaacctcg gcctggcggc cattgccgaa
      181 atccaggcgg cgattgtcga gctgcgtcag taccagccag tgatcgctct gatcgccggc
      241 ccggtcggtt gctttggtgg catgtccatc gccgctggcc tgtgcagcta cctgctggtg
      301 acccgcgaag cccgcctggg gcttaacggt ccgcaggtga tcgagcagga agccgggctg
      361 gatgaatacg attcgcgcga tcgtcccttt atctggagcc tgactggcgg tgagcagcgc
      421 catgccagcg gtttggtcga tggctatgtg agcgatgacg tcgaagcgat tcgtggcgag
      481 ttgcacagtc tgttcgccca gggcaaaccc gagaccgaac gtagccgtcg ccacgcctgg
      541 ttcctcgagc gcctgcgcgc ggtcgacacc tccgttcagg ccgatgccgc tgccgtgcgc
      601 agcgcctacc agggagcaca agcatgacaa ctgcacagga acaacgtggc ctgcactggt
      661 ttcaggcgtt ggccggcacg ccccaagcgc caagcgggtt gcctgcttcg gttcaggtgg
      721 ccgatggtga attggccggg caggccgtgc gttatatcgc cgtggtgcca gatgcggcca
      781 acccctttcc gcgcgctcgc agcggcgagg tcggcctgct cgagggctgg ggcctggccc
      841 aggccgtgga cgccgccatc gaggccgacc gtgaccgccc gcacaagcgc gcactgatcg
      901 ccattgtcga tgtgccaagc caggcctacg gccgccggga ggaggcctat ggcatccacc
      961 aggccctcgc cggtgcggtg gacgcttatg cccgggcacg cttggccggg catgcggtgg
     1021 ttggcttatt ggtaggcaag gcgatgtccg gtgcctttct cgcccatggc taccaggcca
     1081 accgcctgat cgccctgcgc gacagtgggg tgatggtcca cgccatgggc aaggccgccg
     1141 cggcgcgcat taccctgcgc agcgtcgagg agctggaagc gctggccgct gaggtcccgc
     1201 cgatggccta cgacctggac aactatgcct cgctcgggct gctcgccgac atcctcgatg
     1261 tgcagcaggt cgcgcagccg acggcctccg acctggccca tgtgcaggac gccctgagcc
     1321 gtgccctggc cgatatcgcc agcagcccgc gtgatctgcg taaccgcctg ggcgccgcca
     1381 atcgcgcggc gtcatcccag gtgcgcgagc tattacgcgc gcagtggtga acagtgcgag
     1441 cacggtcacc gtagggtgcg ccgtgcgcac caaaaaacct aatcggtgcg cacagcgcac
     1501 cctacagagg acatcttgat gcacccaaac ccccgtccac atgatctgct ctggggcttg
     1561 cgccctgagc acctgcccca ggaggcgccg gcctgggctc gcgtcgcctt gggcgggcat
     1621 gtgccggtgg tggtgcggcg ggcgctggcc gagcctggct gggtggcagt gggtgtgcgc
     1681 ggtgcggcac gcgagcagcg ttatgcgggt tggatgcgca tggcggacat cagccgctta
     1741 gtcagcccgg aagcggtcgc ccgtgcaggt cgctggcgct gttacccgca gtcgcactgg
     1801 ccggcgttac gggcgttgag ccagttggcg ccacaacttg cggaactggg cctagcctgg
     1861 ggcgtcaccg gcagcctggg ctttgagctg gctagtggcg tgtcggctgc gcacctggac
     1921 agcgacttgg atctgctgct acgggcaccg tcgccattgc cccgtgagtg ggcgcgggga
     1981 ttgtgtgagc tgcttgataa agcgccaggg tgtatcgatg tgcaactgga aaccccacaa
     2041 ggtgccgtgg cgctacgcga gtgggccagc ggggctccgc gtgtattgct gaaaacccaa
     2101 gcggggccgc tgctgctcag tgatccctgg cagcctcggc aggtggcggc atgagcacgc
     2161 tttgggcatt tcccggccag ggtgcgcagc agcccggcat gctgcatgac ttgccggata
     2221 gcatcatcgt ccgggcgtgc ctggacgaag ccagcgaggt gctgggcgag tcggtgctgg
     2281 cgctggacac ggccgaggcg ctgcgtgcga cccgcgcggt gcagctctgt ctattgattg
     2341 ccggggtcgc cagtgcccgc ctgttgcaag ggcgcgatgc gcagcccgac tacgttgccg
     2401 ggctgtccat cggtgcctat gccgctgcgg tggtggcggg cgctgtgacc tttgccgatg
     2461 ccctgcgcct ggtcgccttg cgtggtgaac tgatgcagca ggcgtaccct gaaggctatg
     2521 gcatgagcgc tgttctcggc cttgatctgg cccacctgga agcgctgttg gctgaagtaa
     2581 acagcccgca gctgccggtt tacctggcca atatcaatgc cgacaaccag ttgatcattg
     2641 ccggcagcca cgcggccatg gcctgtgtcg ccgagcgtgc gcgccggctc ggtgctggca
     2701 tggtcaaacc cctggcggtt agcgtgccgt cacattgcgc actgcttgcc gagcctgccg
     2761 cccgcctgat cgaggcattc gaagcggtca gcgtgcagcg cccgagtgtg cgttatctga
     2821 gcggcagctc ggcccggttg atcagcgacc ccgaaaaatt gcgcgacgac ctagctcaca
     2881 acatgtgccg ggtggtcgac tggcagggca ccttgcagac cgcgttcgaa cgcggtgttc
     2941 gcctgcatct tgaattaccg ccgggcaacg tactgagcgg cctggctcgg cgcgtattcg
     3001 agcccggtat ggctatcgct tttcaaggtt cgcgcctgga tacactggac gccatgttgc
     3061 gccaggaggt gaatcggagc cgatagcaac acccgattac cgaaggacaa aaacaacaac
     3121 ttcgatgcgt aatacgagga taaaaacaat gattatctac ggtgtggcct ttctggcctt
     3181 ctgcaccctg gcaggcatct ttatcggaga gctgctaggc aaaatgattg gcgtacctgc
     3241 caacgtcggt ggtgtcggta tcgccatgat gctgctgatc ttcctgggca gttacctgca
     3301 caagcgcgga atactcgacg caacgtcgga aaaaggcgtg gcgttctgga gtgctatcta
     3361 catccccatc gtggtcgcca tggccgccca gcagaatgtc tacggtgccc tcagtggtgg
     3421 gccgatggcg attctcgccg gtacggccgc cgtggtcgtg gcctttgccc tggttcccgt
     3481 gctgagtcgt atcgggcaaa aaaagcctga agagtccgca accgcccctc tgactaaagc
     3541 gccacggtga tcgccatgta cgaatcctta atgaaagtac tctccggcta cggcttgctc
     3601 agcggtttcg ccattatcgg cctcaccatg tgggtgtcct actggatctc ggacaccttc
     3661 accaaaggcc gtttgcatgg gtcggcgatt gccatcctgc tgggcctgct gctgtcctat
     3721 atcggtggtg cctttaccgg cgggaccaag ggcttggtgg atatcccgct gtttgccgga
     3781 gtcggtctgc tcggtggggc catgttgcgt gactttgcca ttgtcgccac tggttttggg
     3841 gtaagcgttg aagaactcaa acgtgcaggt tttgccgggg tgctggcgtt atttgtcggc
     3901 gtgggcgctt catttgttgc cggggtcggc gtggccatgg ccttcggtta caccgacgcg
     3961 gttagcctga ccactatagg taccggtgcg gtgacctata tcgtcggtcc ggtgacgggg
     4021 gcggctatcg gtgccagttc ggaagtcatg gcgctgtcga ttgctgccgg tctggtcaag
     4081 gcgatcctgg tgatggtggc gacacccttt attgctccct atatcggcct gaacaaccca
     4141 cgcagtgcgg tgattttcgg cggtctgatg ggcacctcca gcggtgtggc cggcggcctg
     4201 gcggcaaccg atccgaagtt ggtgccctat ggttgcctta ccgcggcgtt ctataccgcc
     4261 ctgggctgcc tgctcggccc atcgctgttg tactttctca tgcgcggcct attcggctga
     4321 gccttcgccc gccgtttggc gggctttttc ttcctgtagg gtgcgcgtat aggcggcatt
     4381 cggcgagcgg gtagggtgcg ccgtgcgcac cgcggtgaac ccgtataagc ggcccctacg
     4441 actggcgact gtacatccgg cattcggcca gcagcgccaa gaggttcggg tcgcgctcct
     4501 tggctttcag gaagaccacg ccgatgtgct gttgcaggtg gtacttggct tgcaacggga
     4561 tcaggcgcac gcggttttcg tacaccgcag caatccgccc cggcagcaag gcgtaaccca
     4621 cccccgagct gaccatgctg agcagggtga agatatcgtt cacctgcatc gccaccttgg
     4681 gctcgaagcc cgcctgctga aacacccgca cgccgtcctg gtgggtggcg aagccctggg
     4741 tgagggtgat aaaggtcgag tcgcgcaggt ccgccagatc cacttcggct tgcatggcga
     4801 agggcgagtc gatgggtacc gcgaggaaaa tatcatcggc aaacagcggc agctgctcgc
     4861 agtcgggatc gctgacgctg tcattgagcg ccaccagaat ggcgtccagt tcgacgttct
     4921 tcagcttgta cagcaggtcg acgttggagc cgagaatcag gtcgatattc agctcgctgc
     4981 ggcgcagctt caggcccatg atcagctgcg gtacggtttt taccgtcagt gaatacagcg
     5041 cgccaagctt gaaacgttcg gccgaaaagc cggccgcctc gcgggtcatg cgcacgcttt
     5101 cgactacgtc ttggaccagc ttctgcgccc gctcttccag cacgtaggcg ctttccaggg
     5161 ggattaggtt gcggccttcg tgtttgaaca gtgggcagcg cagggcgctt tccagtgagt
     5221 gaatggcgcg gtgcacgctg acgttgctgg tattcagctc gacggcggcg cgcgtcaggt
     5281 tgccgctgcg cataaaggcc agaaagattt ccagcttctt cagggtgagt tcttcgtcga
     5341 tgagcatcgg gtctggcctg aggaggagta ggccaagtgt gccgtattgg cgcggcacac
     5401 gcgctcagcc gttagtggta gctcaaccgc acgtcaggcg gatgatcacc tcgctctcat
     5461 cgatatcgat attcaggcgc tgcgggttgt aatccatggt ggcggcgtcg ccgggggcga
     5521 gtacgcgcag gaatttggcg ttggcctgtt gttgcgcctg cttgatcatc tcggcgctgg
     5581 cgttttggcc gagtacatcg ctcatgttgt cgacattgca gtcgccggtg atttcagcgg
     5641 ctggctgcgt tggctgctgc gagctgcaac cgaccagtag ggcgagcagg gtaaggctgg
     5701 cgaggtggtg acgaagagcc atggtctatt tccttgtctg ggtaaaacca ggtccagcct
     5761 agcagccgca tccgctctag ctcaacgttg gccttgaaaa acatcccagg ttgctgcatc
     5821 agcatttccg caagcccggc caacttccct acaattgcgc ttttccccag agcaggaggt
     5881 tctgtgcaaa cggtgatctc gattcaacag ctgagcaaga cctacgccag cggccatccg
     5941 gccctgcagg acatcaatct ggacattcgc cccggcgaaa tctttgcgct gctcggcccc
     6001 aatggtgcgg gcaagaccac cctgatcagc attatctgcg gcatcgtcaa tccaggcggc
     6061 gggcgggtgc tggtgggcgg caaggacatt atcaaggact accgcgcgtc ccgttcacag
     6121 atcggcctgg tgccgcagga gctggtcagc gatgtgttcg aaaccgtctg ggcgacggtt
     6181 aagttcagcc gtggcctgtt cggcaagaag cccaatccgg cctatctgga gcaactgctc
     6241 aaggacctgt cgctgtggga caagcgcgac gcgaagattt tcgagctgtc cggcggcatg
     6301 aagcgccggg tgatgattgc caaggcgctg agccacgagc cgcagattct gtttctcgat
     6361 gagcccaccg ctggcgttga cgtcgagctg cgccgcgata tgtggaacat ggtgcgccgc
     6421 ctgcgtgagc gcggcgtgac cattatcctc accacccact acatcgaaga ggccgaggaa
     6481 atggccgacc gcatcggggt gatcagcaag ggccggatca tcctcgtcga agacaagcag
     6541 gcgctgatgc acaagctcgg caagaagcag ctgacgctgc agttgcaaca gcccctggcg
     6601 agcattcccg ctgagctggc gcgctacccg ctggagctga tcgatgaggg ccacgccctg
     6661 gtgtttacct tcgacaccca gagcgagcac accggcattg ccgaactgct caaggacctg
     6721 gctcagcacg gcatcgactt caaggatttg cagtccagcc agagttcgct ggaagaaatc
     6781 tttgtgtctt tggtcaagga gtcgcgcgca tgaatttcta cgcaatccgt gccatctacc
     6841 tgttcgaact ggcgcgcacc tggcgcaccc tgctgcaaag catcgccacc ccggtgatca
     6901 gtacttcact gtacttcgtg gtgttcggtt cggccatcgg ttcgagcatg acccaggtac
     6961 agggcgtcag ttaaggcgcg tttatcgtgc cggggctgat catgctggcg ctgctgaccg
     7021 agagcatttc caacgcctcg ttcggcatct acatgcccaa gtattcgggg agcatctacg
     7081 agctgctgtc cgcgccggtg tcctacctgg aaatcctcgt cggctatgtc ggcgcggcgg
     7141 ccaccaagtc aatcattctc ggcctggtga tcctgattac cgcgcggctg tttgtggatt
     7201 tcgagattct tcacccgatc tggatgctgg cgtttctggt gcttaccgcg ctgaccttca
     7261 gcctgttcgg cttcatcatc ggcgtgtggg ccgacggctg ggagaagctg caggtggtgc
     7321 cggcgctgat cgtcacgccg ctgacctttc tcggtggcgc gttctactcg atcagcatgc
     7381 tgccaccggc ctggcagacg gtgaccctgt tcaacccggt ggtgtacctg atcagcgcgt
     7441 ttcgctggag tttctacggc gtctccgacg tcaacgtcgg cctgagcctg gcgatgattc
     7501 ttggctttct gctgctgtgc attctggctg tgggctttat cttcaaaacc ggttatcggc
     7561 tgaagagctg aagtgcgcct aatgcgttag ggtgaaacgg ttatggttgc gcggcagggg
     7621 tgttgcgcag ccactgccgt tatggagcgc aatgccatgg accattctgc cgccttgtcg
     7681 gccctgctgg cgcagctcta ccgcgatgaa tcgcgtcgcg tgctggctac cctgatccgt
     7741 ctgctgggcg atttcgacct ggccgaggag gcgctgcacg aagcctttcg cgcggccatg
     7801 gagcaatggc cgcagactgg tgtgccggcc aatccacggg cctggctggt gtcggcaggg
     7861 cgcttcaagg ccatcgacaa cctgcgccga cagcggcgtt ttcagccgct ggatgaacac
     7921 gttgagctgg tcgatgacgc gccgctgggc gagggcgagt tgcttgaaga cgaccgcctg
     7981 cggctgatct ttacctgttg tcacccggcg ttggccagcg atgcccaggt ggccctgact
     8041 ctgcgcgaag tctgcgatct gggcaccgag gaaattgccc gcgcctttct cactacgcct
     8101 gccaccctgg cgcagcgcat cgtccgcgcc aaggcgaaga ttcgcgatgc gcgcattccc
     8161 tatgaagtgc ccggacgcag cgagctgcct gaacgcctgg acgctgtatt gcgggtgatc
     8221 tacctggtgt tcaatgaagg ctatttcgcc agctccggcg attcgctgac ccgcgcccat
     8281 ctgtccgacg aagccatccg cctggcgcgc ctgctgctgg agctgctgcc cgagccggaa
     8341 gtgcagggcc tgctggcgct gatgttgctg catgaatcgc ggcgcagcgc gcgcagctcg
     8401 gccaacggcg aagtggtact gctggaggcg caggatcgtc gccgatggga tcgtgcgctg
     8461 atcgccgaag gcgacgcgct ggtgctgcag gctctgcatt cgcggcgttt cggcccttac
     8521 accctgcaag ccgcgattgc cgctgtgcat gccgaagccg cccacgccga gcagaccgat
     8581 tgggcgcaaa tcgtcgggct ttacgatgct ttgctacgcc tgaatccatc accggtaatc
     8641 gagctcaacc gcgccgtggc ggtggccatg cagcaaggtc ccgaggccgg attggcactg
     8701 atcgacgcgt tgctggccag tggcgaattg gccgattacc accttgccca cgccgctcgc
     8761 gccgatctca atcgccgcct gggccgcgta gcgcctgcgc gcgatgctta tgagcgggcg
     8821 cttgagctgg cccagcaggg cgcggatcgg cagtttctgc agatgcgcct ggacgagttg
     8881 ccgaccggct aagccgccat ccgcctgacg gataagcgct gcttggctac cgttggtcgg
     8941 gcctgaaaat attttgtgcg gcgctgtcga ttcagcgccg tgccattcga ttagtcagta
     9001 accgcgccac accaccggag aacgccatga aatacctgtg tctggtctac agcaacgagc
     9061 atgaactgca caactcgccc gacagcccgc gtgacgagga atgtttcgcc tatgcggagt
     9121 cggtagcaga aagcggccgc atgctcgccg ccgaagcgct ggagtcggtg cagaccgcca
     9181 ccacggtgcg catgcgcggc ggcaagctgt cgatcatcga cgggccgttt gccgagacca
     9241 aggaacagct ggctggtttc tatctggtgg aagcccgcga ccttaacgaa gctattcaac
     9301 tggccgggca tattccggcg gcgcgcgtcg gttgtgtgga agtccgcccg gtgcgcgagc
     9361 tgcaggtcta gagcgcacgc tgcccgcgtt gctcaatcaa cggaaaagga gcactgcata
     9421 tgagtcgtcc tgaatacccc aagattgcca gccgtgagca gtggctgatt gcccgcaagg
     9481 ccttgctggc caaagagaag gcgctgaccg agcagcgcga tgcgctgaat gttgagcgcc
     9541 gtcagctacc gatggtcaag gtcgaggccg attaccggtt tcaggggcct gagggtgaag
     9601 taggtctggt cgacctattc gccgggcgca gccagctgat cgtctaccac tttatgtacc
     9661 acatggaccg tggcgagggc tgcgacggct gttcctgcgt ggccgacaat atcggccacc
     9721 tggcccacct gcatgcccgc gataccaccc tggtgatggt ttcccgcgcg ccactggctg
     9781 atctgcaggc gttccagcgg cgcatgggct ggactgtgcc ctggtattcc tcgctgggca
     9841 gtgcgttcaa ctacgactac cacgccacga cggacgagca ggtcgctccg gtcgaataca
     9901 actaccgcga caaggccgag ctggagcgcc tgggcctgaa gtaccacgtg cagggcgagc
     9961 agcccggcgt cagcgtattt ctgcgcgatg gcgcgcaggt ttatcactgc tactccagct
    10021 atggccgtgg cctggaaatg ctcatgagca gcttccacta cctcgatctc acgccattcg
    10081 gtcgtggtga aggctggggc ggaatgcccg atctggaggg caagggcctg cactggaccc
    10141 gactgcacga cgaataccag caaccggcag cagcgcatga ctgctgcgcc tcaatcaagc
    10201 cctaaacgca aggagtacga ccatgagcaa cgcacaagcg caaattcaac agcaactgga
    10261 ctcctgggcc gccgcctgcc gcagtaagga cgtcagccag atcatgcgtc actacgccga
    10321 ggacgtggtg gcctacgacg ccatcggccc gctgcgcttc cagggccgcg cggcctatca
    10381 ggcgcattgg caggcctgta tggaaatgtg cagtggtcag gccatgttcg agattcatca
    10441 gccgagtttt ctgctcagtg atgggctggc tgtggtgcat tacctgttcc actgtggcgg
    10501 caccgatgac aaaggtgaaa tgcaaagcag ctggatgcgc gtaagccagt gtttccgcca
    10561 gcagggcggg caatggctga tcgcccatga gcatttttcc atgccgtgcg acatggaaag
    10621 cggaaaagtt ttgtttgatt tgcagccgta gccttgtaac tcacagggca caaggggtgt
    10681 agcgaattgc aggagggtac gttgcagatt tgtggttacg accaacgtct tagtgcttgc
    10741 ctaaaggggg gcttagtggc gtaggctgga gatgtcgcct tgcttggcga cgctttcgcg
    10801 aacagttgaa gaaggaaagc tttatgcaaa tccaagttca cagcgataac cacatcgaag
    10861 gcagtgctcg tctggttgag tgggtgagtg ccagtgttac cagcaagctg gagcgcttcg
    10921 atgacgagtt gacccgcatc gttgtccatc tgcatgacga caacggcgcc aaagccggcg
    10981 cgcatgacaa gcgctgccag atcgaagcac ggccaaaggg cctgcaacct atttccgtca
    11041 cccacaaggc cgagtcactg gaacaggcca tcgacggctc catcgaaaaa ctgcaccacg
    11101 cccttgagca tcaattcggc aaactgcgca gc
//
LOCUS       DASRUM010000267        20377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_475830, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000267 DASRUM010000000
VERSION     DASRUM010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 20377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20377
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475830"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..116
                     /locus_tag="VFF22_18050"
     CDS             <1..116
                     /locus_tag="VFF22_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine-zipper protein"
                     /protein_id="HZX18758.1"
                     /translation="EAYRKADDAMAAAQKAQQTADEANERALRMLEKASRK"
     gene            complement(252..1205)
                     /locus_tag="VFF22_18055"
     CDS             complement(252..1205)
                     /locus_tag="VFF22_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZX18759.1"
                     /translation="MLSRALAVTRCLPLAAVFAVASANALEFPLPPPGEDIVGEVQVI
                     QAKYEDTFADLGVANDLGYLEMVVANPGVDAWLPGEGSEIILPTRYILPPGPREGIVI
                     NLAEYRLYYYPKGRDVVYTYPLGIGREGWSSPVANARITAKTPNPGWTPPKSILAEHA
                     AAGDPLPAYVPPGPDNPLGPYKMGLSVPGYLIHGSNKKFGIGMRVSHGCFRMLNHNVL
                     KLASMVSVGTPVRIINEPYKFGRSGGKVYLEAHAPLDDSGDASIVDKHTAVINALLKN
                     DQFADLRLDWEMLREVVAAEDGLPVEIAQPDQAMVAAEPSI"
     gene            complement(1282..1563)
                     /locus_tag="VFF22_18060"
     CDS             complement(1282..1563)
                     /locus_tag="VFF22_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012313663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18760.1"
                     /translation="MPRPAWSLHAYQLIEPDEQLDLFACREVRVHLLARQLELGQFAD
                     RTLCGGLLPAQPVWRDLDKLMLRDKRLCPACRATLEAEKRGDRPLLPGL"
     gene            complement(1622..2227)
                     /locus_tag="VFF22_18065"
     CDS             complement(1622..2227)
                     /locus_tag="VFF22_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017134263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HZX18761.1"
                     /translation="MRKWLIGTALSLLLWGPAAFAGTVLVVGDSISAAFGLDTRQGWV
                     ALLEKRLTEQGFDHRVVNASISGDTSAGGAARLPALLAEHQPELVIIELGGNDGLRGQ
                     PPAQLQQNLAAMVQASQQKGAKVLILGMQLPPNYGVRYTTAFAEVFPKVAQEYDTALV
                     PFVLEGVGGVPAMMQSDGIHPTAEAQPKLLENVWPNLKPLL"
     gene            2238..2921
                     /locus_tag="VFF22_18070"
     CDS             2238..2921
                     /locus_tag="VFF22_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX18762.1"
                     /translation="MSSSILTARNLSKVVPSTEGELTILHDLSLELISGDSLAIVGSS
                     GSGKSTLLGLLAGLDLPSSGSVVLAGNDLASLDEDQRARVRAEHVGFVFQSFQLLDSL
                     NALENVMLPLELEGRADARQRARTLLDRVGLGQRLTHYPRQLSGGEQQRVAIARAFAA
                     EPAVLFADEPTGNLDSHTGERISDLLFELNKERGATLVLVTHDERLAHRCQRLIRLEG
                     GHLVDHVEP"
     gene            2921..5419
                     /locus_tag="VFF22_18075"
     CDS             2921..5419
                     /locus_tag="VFF22_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX18763.1"
                     /translation="MNHLPFTRLLSLASRQLLRDARAGELRVLFFALLIAVAASTAIG
                     YFSARLNDGMLLRATEFLAADLRLTGSSPSAPEQISSGKQLGLEHAQLVEFSSVVASD
                     AGIQLASVKAADNAYPLRGELKSTAALYDVEQTGAGPQPGEAWAEARLFVALNLKPGD
                     SIEVGAKTLRLTRVLTFLPDEAGDFYSLTPHLLMHLDDLAATGVVQPGSRVRYQELWR
                     GEADALAQYRQGITLAPNQRLEDAKDGNRQVGSALGRAERYLNLASLAAVLLAGVAVA
                     LSAARFAARRFDASALLRCLGLSRNQALGLFSLQLALLGITASLIGALLGWLAQLALF
                     NLLQDLLPAQIPPGGLWPALAGMATGLVALAGFALPPLAALGRVPPLRVLRRDLLPVP
                     ASSWLVYGAALLALGLIMWRLSLDLQLTFALLGGGLVTALLLGGLLLLGLQSLRRMLA
                     GASLPWRLGLGQLLRHPLAAAGQSLAFGLIILAMALIALLRGELLDTWQDQLPENAPN
                     HFALNILPADKDSFSARLSELSPHPAPLFPVVPGRLIMINGEPVRQIVSKESQGERAI
                     RRDLSLTWAAELPADNNLIAGSWWTDANASELPGVSVESELAESLQLKLGDRLSFSVG
                     GLNRDAVVSSLREVDWNNFQPNFYMIFQPGTLQDLPVTYLTSFYLPPQYEKQLVELSR
                     AFPAVTLLQVEALLAQLRSILAQVTLAIEFVLLFVLAAGLAVLFAGLQATLDERIRQG
                     ALLRALGAERKLLIKARRAEFGLLGASSGLLAAFGCELISFLLYRFAFDLAWQPHPWL
                     LLLPLIGALLVGTAGVLGTRRALNISPLTVLREG"
     gene            5453..5950
                     /gene="greB"
                     /locus_tag="VFF22_18080"
     CDS             5453..5950
                     /gene="greB"
                     /locus_tag="VFF22_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HZX18764.1"
                     /translation="MSRYRPPRSAGTALITPEGEARMRAELHELWHVRRPQVTQSVSE
                     AAAQGDRSENAEYTYGKKMLREIDSRVRFLTKRLENLKVVSARPSDPDKVYFGAWVTL
                     EDEDGSESRYRIIGPDELDLKNNLISIDSPLARALIGKSLDAEVRVQTPTGEKTWYIV
                     AIDYL"
     gene            5962..7941
                     /gene="mnmC"
                     /locus_tag="VFF22_18085"
     CDS             5962..7941
                     /gene="mnmC"
                     /locus_tag="VFF22_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014337204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HZX18765.1"
                     /translation="MPSAPDVPTQQARLEWDEQGQPLSSQFADVYFSNENALAETRYV
                     FLANNQLPERFAALTADQKMVIGETGFGTGLNFLCAWQLFEQQAAAGARLHFVSVEKY
                     PLNKADLQRALDLWPELAPYAEQLLAQYVALHPGFQRLVFAGGRIVLTLLIGDALQLF
                     GQLDAQVDAWFLDGFAPAKNPDMWTPELFAELARLSHASTTLGTFTSTGYVRRRLNDA
                     GFKMKRVPGLGKKWEVLKGSFIGTAEATEKPWFARPPQPNGPRTAIVIGAGLAGCATA
                     ASLAQRGWQITLLERHNAIAQEASGNPQGVLYLKLSAHHTTLSRLIVSGFGHTRRLLE
                     RLHKGQDWDNCGVLQLAFDAKEAQRQAQLAAAFPADLLTNLDRNAAEIRAGITLPAGG
                     LFYPEAGWVHPPALCELLSQQPTIQLQLHQEALELRRDGDSWQAWNGEQLLASASVVV
                     LASAAEIKGFNQAAHLPLKRIRGQISRLPATAASRALSTVVCAEGYVAPVRQDEHTLG
                     ASFDFNSDDLTLNSADHASNLHLLEEISPELANRLNVAALSPEQLQGRAAFRCTSPDY
                     LPIVGPLADAAAFAEAYAVLSKDARQVPETPCPWLDGLYINSGHGSRGLITAPLSGEL
                     IAAWLNDEPLPVPADVAQACHPNRFSLRALIRNKS"
     gene            complement(7974..10247)
                     /gene="rlmKL"
                     /locus_tag="VFF22_18090"
     CDS             complement(7974..10247)
                     /gene="rlmKL"
                     /locus_tag="VFF22_18090"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HZX18766.1"
                     /translation="MSDRYELFLTCPKGLEGLLLEEASSLGLEDTREHTSAIRGMADM
                     ETAYRLCLWSRLANRVLLVLKRYSVQDAQSLYEGVLEVDWFEHLESTGSLAVEFSGNG
                     SGIDNTHFGALKVKDAIVDKLRLKDGTRPSVDKLNPDLRIHLRLDRGEAILSLDLSGH
                     SLHQRGYRLQQGAAPLKENLAAAVLIRAGWPRIAAEGGALADPMCGVGTFLVEAAMIA
                     ADIAPNMTREQWGFSKWLGHVPALWKKVHAEAEQRAAAGMAKPPLWIRGYEADPRLIQ
                     PARNNIERAGMSDWIKVYQGEVATFEPRPDQNQKGLVICNPPYGERLGDEASLLYLYQ
                     NLGERLRQACMGWEAAVFTGAPDLGKRMGIRSHKQYAFWNGALPCKLLLIKVLPEQFV
                     TGERRTPEQREREREQAEADKSPLEPLERQYNKNGNPIKPTPAPAPVVEQARLSEGGQ
                     MFANRLQKNLKQLGKWARKEGVECYRLYDADMPEYSMAVDLYGDWVHVQEYAAPKSID
                     PEKAKERMFDAIAAIPQALGVDKNKVVIKRRERQSGTKQYQRQATQGQFMEVSEGGVK
                     LLVNLTDYLDTGLFLDHRPLRLRIQKEAAGKRFLNLFCYTATATVHAAKGGARTTTSV
                     DLSKTYLDWARRNLSLNGYSDKNKLEQGDVMTWLAEDRGEYELIFIDPPTFSNSKRME
                     GIFDIQRDHVQMLDLAMARLAPGGVLYFSNNFRKFVLDESLAARYRVEEISESTLDPD
                     FARNQKIHRAWRISLRG"
     gene            10671..10886
                     /gene="rmf"
                     /locus_tag="VFF22_18095"
     CDS             10671..10886
                     /gene="rmf"
                     /locus_tag="VFF22_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HZX18767.1"
                     /translation="MRRLKRDPLERAFLRGYQYGISGKSRELCPFTLPTVRQAWLNGW
                     REGRGDNWDGMTGTAGIHRLNELHAVG"
     gene            complement(10967..12004)
                     /locus_tag="VFF22_18100"
     CDS             complement(10967..12004)
                     /locus_tag="VFF22_18100"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HZX18768.1"
                     /translation="MYTLARELLFKLSPEASHELSIDLIGAGGRLGLNGLLTKSPAAL
                     PVKVMGLEFPNPVGLAAGLDKNGDAIDGFAQLGFGFVEIGTVTPRPQPGNPKPRLFRL
                     PEAEAIINRMGFNNHGVDHLLARVQAAKYKGVLGINIGKNFDTPVENAVDDYLLCLDK
                     VYQHASYVTVNVSSPNTPGLRSLQFGDSLKQLLEALRKRQEDLAAQYGKRVPLAIKIA
                     PDMSDDETIEVANALVEAGMDAVIATNTTLGREGVHGLECADEAGGLSGAPVCEKSTH
                     IVQVLAGELKGRLPIIAVGGITEGKHAAEKIAAGASLVQIYSGFIYKGPALIREAVDA
                     IAALPQKYQGQ"
     gene            complement(12085..12306)
                     /locus_tag="VFF22_18105"
     CDS             complement(12085..12306)
                     /locus_tag="VFF22_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2835 domain-containing protein"
                     /protein_id="HZX18769.1"
                     /translation="MPSLVFDIALSAERLRVVYQGRANRILVQSRDGRSVSVPAHHFR
                     PFLTHDGVYGSFELEFSPVGELLSLRRLG"
     gene            12457..13329
                     /locus_tag="VFF22_18110"
     CDS             12457..13329
                     /locus_tag="VFF22_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6685 family protein"
                     /protein_id="HZX18770.1"
                     /translation="MSLSEQPSTLSSRLGALAQRLGLIGRGQRQILERASQLRLPFSP
                     LPQLRNSICWQDGPQLHRLIDLPRSALSGPVQEDKAQAHAMLVRVIEAQEQQLTSFDL
                     RMIDGLSTCNPQQANHTSFEVFAASETCRQVRIISYKDFVKAISQALPRFLAGERIIL
                     RQASWRGERVFWAGEHHSEAFACAIAYARRRELEVNLPAELSHYRLSEQGLSNLQQHY
                     HVLGMPNAAWSDPAFMALLLDNGLPYARLSLLGAPGSPEFLLLPKHSSEATALGEGLR
                     LAGAPDVVGYLQQL"
     gene            complement(13432..>20377)
                     /locus_tag="VFF22_18115"
     CDS             complement(13432..>20377)
                     /locus_tag="VFF22_18115"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5801 repeats-in-toxin domain-containing
                     protein"
                     /protein_id="HZX18771.1"
                     /translation="AAAGDDGTVYSLQLSGADVPSGLFAVDASAPLGKGEQIVLNQLG
                     NVITGSAGGVPYFTLAIDPVSGEVTLELLDNLWHDDTADADDNEVLALAEGALLLTQT
                     VTDGDGDSDSASIDLGASGVFSFEDDGPVAGKTAAAVVLDDEGLLGGINGGPGDVAGA
                     ATSTSGNLAFNAGADGLKSIVLSGPISLGSEAVTSNWDAPTNTLTISSVRGALLTVVL
                     TDPASGAYTINLLQPLMHPVSGTEDDITLNIGYTVTDGDNDSATGSLVVTIDDDTPTI
                     QAGNIGVTSFVTFQGTDAGFSNSYGYYNKAPDGSPVDGKVIWANVHAQSVGDVADLSG
                     LDPANTGFFIIPNGGANADLTNGSVVTFEFVAGQWQAKLGGVPLVGADGANVLFSDAS
                     LNPGGSHLQDTGNAGNQNWEDQTLTSDYDYNDVSTNVTWGSSLQVDESDFSADATANF
                     SGVFNVQPGADGLHALTPLSYNLSVQNASSGLVDTETNQAVILSLNAGVIEGRTSGTN
                     QLVFTLSVDATGTVTLDQIRAIVHPTGDPDEAKFLGAGHVSLNAVVTDGDGDQASASI
                     DIGTVISFKDDGPSVLPNALVQLDDDALANGIPGGIGDDDNAVEISGTLGLNFGADGA
                     GNVQWLTSGAPADFTYAAGPGGSLLISQGTTLVLTVTLNSASGAYTVTQNAPILHAEA
                     DQENNQAFILNYRVTDKDGDTADGTLAINVDDDTPVAQDDVAYVSTGQTENINMVFVL
                     DFSGSIDNTELNLMLDAVRTAGQELFNNAAGAVQIQIVAFSHDSIGYPVSSDITTFTN
                     LVNSLNPSEPGGVRPLDNTTDFTDAIQQTMVSFVPQPGWNNQVVFISDGNPNEQTGTG
                     GHSLTDTTAAVWNTFVDTNGITVTTIGVGNGIIDQRLENVDMDAGPNNDPLRADDFDD
                     LVDTLLDNVVGGLVNGNVLHGSDGVDNGGAGDDDGYGADGPGRIMSIEINGITHTWDG
                     VLDGGQILSNIPTTHGGKLDFNFATGAWIYKAPSSGGGDKVETFSYVIVDKDGDPSEA
                     TLTVYVEDISPVIAKVVEDELPGGITDSDGETTIATGSVSNLVVGPSVGVLFSLSGNT
                     SGLDGASSGGTPLVYSVVGDTLSAMAGTDTVFTLQVESDGDYTFTLLKPLDHPLGNGD
                     DDELLVINFASILQASNGINPVPLAGSFLVQIEDDVPTVSANAPVQLDDDALANGIAG
                     GPGGTDDADSVNAGGTLQHNFGADGAGTVKWLESGAPGGFTYQASPDGSQLLVKQGAT
                     TVLTLTLNTGTGVYTVTQNAPIQHANANQENNQAFNLSYQVTDKDGDKATGTLSINVD
                     DDTPLAKNDTADVAEGRGQDFNVAFVLDSSGSISNSEFSTMMNAVKAAGQALYTGTDG
                     DVKVTVVAFSSTSLAYPAVTTLASFNALIDNIIANRPFSGQTDFTDAIEETMAVYSPI
                     PSWSNQVFFISDGNPNQQTDPSDSSLTTSTATAWNSFVDNSGINVTTIGVGDGINAAR
                     LQDVDVDGNGSPILISNFNGLIDTLLSQLVGGGVSGNVLLGSDNAVGGGDDDSFGADG
                     PGYIQSIVINGATYTWNGVGTISVSTGGNIAGNQLTEIATGLGGKLSFNFATGAWSYM
                     APPSVAADTSESFGYTIVDKDGDPSSASLTINIEDASPIIGRVDEDELPGGITDGDSV
                     NTVATGSLVDLLVGTNNGQFSLNTSPAIMPTITSGGVAITYSFSGDTLTAKAGSTDVF
                     TLKVMSDGTYTFTLLGPLDHPSAGTNDNEILTLNLTNAIKASNGVNPLPIAGDLLIQV
                     EDDVPIAFTPVTALLVDQVSETRSITADLRFEASVGADGPDQVIFSIVNGAAARDASG
                     NLLSMGGQQLYLFYGSDQSIIEAKTSANGNLGYRIDMDAATDSYTLTTYGPIANGSEV
                     SATNLTGVGGGNVPFKGLIDVGGSTFDALLSTTVGQTVNSNSTEVGIGTGNSFSDGER
                     LRIDLVNGLRQGGSNGTGFLYDSHNTTNRFAQQFDSVNPSGSGTAAFWISVLLADNDY
                     SFIGDSGDAYINLSTNNIRIFAIENGVEVNKASEVTIQDTGNWIRISGMEKGWWYEVN
                     STTAFNAVQIEGDNEQPFKLGFFNYDQASTGQPINLSHNIIGIDGDGDSVSSTLKATL
                     VPDSLSVDGGAGVDNLEGTAQADYLFGNGGNDTLSGLEGYDILSGGAGDDILIGGLGD
                     DVLAGGAGADIFRWLSTDITGADKVLDFNPSEGDKLDLTQLLIGESYNAGSLDDFLSF
                     SVVDNSTIIAVSPTAGGVATTTVELTGFNVAVEYGVTPGGGGIISGGTDTASVINGLL
                     GDNALQVV"
BASE COUNT         4276 a         6354 c         5664 g         4083 t
ORIGIN      
        1 acgaagccta ccgtaaggct gatgacgcta tggctgctgc tcagaaggct cagcaaactg
       61 ctgacgaagc caatgagcgc gctctgcgta tgctggaaaa agccagccgc aagtaatagc
      121 tcacaaggct atgacaagcc ggcccagttt ctgggtcggc ttttttattg ccaagcgaaa
      181 tacccattat tcaacaaaca ataaaaagcc cgtcagcgct cacgcataac gggcttttag
      241 aacagccgac gtcagataga cggctcagcc gccaccatgg cctgatcagg ctgagcaatt
      301 tcaaccggca aaccatcttc agcagcgacc acttcgcgca gcatttccca gtccaggcgc
      361 agatcagcaa actgatcatt tttcagcagc gcattgatta ctgcagtgtg cttatcgacg
      421 atcgaagcat caccggaatc atccaaaggt gcgtgcgctt ccagatagac cttgccgccg
      481 ctgcgaccaa acttgtaagg ctcgttgatg atgcgtaccg gagtgcctac agaaaccatt
      541 gatgcaagct tcagcacgtt gtggttaagc atgcggaagc agccatggct aacgcgcatg
      601 ccaataccaa atttcttatt cgagccgtga atcaggtaac ccggcaccga caagcccatc
      661 ttgtatggcc ccaatgggtt atccgggcct ggcggcacat aagctggcag cggatcacct
      721 gcagccgcat gctctgcaag aatcgacttt ggcggcgtcc agcccggatt aggtgtcttg
      781 gcggtaatac gcgcattggc aaccggcgag ctccagcctt cgcgaccaat acccagtgga
      841 taggtgtaaa ccacgtcacg acctttcggg tagtagtaca ggcggtactc ggcgaggttg
      901 atcacaatac cttcacgcgg gccaggcggc aggatatagc gcgtcggcaa aataatctcg
      961 ctaccttcac ccggcaacca cgcatcaaca cccggattgg ccaccaccat ttccagatag
     1021 cccaaatcat tggccacccc cagatcggca aaggtgtctt catacttggc ctggataacc
     1081 tgcacctcgc caacgatatc ctcgcctggc ggcggcaacg gaaactcaag cgcattggca
     1141 gatgctaccg cgaagacagc ggcaagcggc aggcagcggg tgacggcaag ggcacgcgac
     1201 aacatccggg aattcctgac gaaaaaaggt tggtaaaagt agggaacgat tgtacaccga
     1261 ttgatcagct gccgggacag ctcaaagccc cggcaacaga gggcgatcgc cacgcttttc
     1321 tgcttcgagt gtcgcccgac aggccgggca caaacgtttg tcgcgcagca tcaacttgtc
     1381 cagatcacgc cacaccggct gcgcaggcaa caaaccaccg cacaacgtac ggtcggcaaa
     1441 ctggcccaac tcaagctgac gcgccagcag atgcacgcgc acttctcggc aggcgaacag
     1501 gtcaagctgc tcgtcaggct cgatcagttg gtaggcatgc aaggaccagg cagggcgtgg
     1561 cattgagggc tccagtgcgg ggccgccaca ttagccgaaa ggcaccttac agaaaagcct
     1621 gtcagagcaa aggtttcagg ttcggccaga cgttttccag cagcttgggt tgcgcctcgg
     1681 cagtgggatg aatgccatca ctctgcatca tcgcaggcac gccacctacg ccttccagca
     1741 cgaaaggcac cagggcagtg tcgtactcct gcgccacctt ggggaatacc tcagcaaacg
     1801 ccgtggtgta acgcacaccg tagttaggcg gtagctgcat gccgaggatc agcaccttgg
     1861 cacctttttg ctgcgatgcc tgcaccatgg ccgcaaggtt ttgttgcaat tgggcagggg
     1921 gctgaccacg caggccatcg ttgcctccca actcgataat caccagctct ggctgatgct
     1981 ctgcaagtag cgcaggcagc cgtgcggcgc cgcctgcgct ggtgtcgcca ctgatcgaag
     2041 cattgaccac tcgatggtca aaaccttgct cagtcaggcg tttttccagc aaagcgaccc
     2101 aaccctggcg ggtatccagg ccaaaagccg cgctgatact atcgccgacg accagtacgg
     2161 tacccgcgaa cgccgccggc ccccacaaca gcaggctcag ggcagtaccg atcaaccact
     2221 tacgcattgg attctccatg agctcaagta ttctcaccgc gcgaaacctt agcaaagtgg
     2281 ttcccagcac ggaaggcgaa ctaaccatac tccacgatct gtcgctggag ctgatttccg
     2341 gcgatagcct ggcgattgtt ggcagctccg gctcgggaaa atccaccctg ctcggcctgc
     2401 tcgccgggct cgatctgccc agcagcggca gtgttgtgtt ggctggtaac gacctggcca
     2461 gcctcgatga agatcaacgc gcgcgcgttc gcgccgagca cgtcggtttt gtcttccaat
     2521 ccttccagct gctcgacagt ttgaatgccc tggaaaacgt catgctgccg ctggagcttg
     2581 aaggccgtgc cgatgcccgc cagcgcgctc gcaccctgct cgatcgcgtc ggcctgggcc
     2641 agcgtctgac ccattaccca cgccagctct caggcggtga acagcaacgc gtggccatcg
     2701 cccgcgcctt tgccgccgag cctgcagtgc tgtttgccga cgagcccacc ggcaacctcg
     2761 acagccatac cggagagcgc atcagcgacc tactgttcga actgaacaag gaacgcggcg
     2821 cgaccctggt actggtcact catgacgagc gcctggctca tcgctgccaa cgcctgatcc
     2881 gcctggaagg cggccacctg gtcgaccacg tggaaccctg atgaatcatc tgccgtttac
     2941 ccgcctgctt tccctcgcca gccgccaatt gctgcgcgac gcccgcgctg gcgagctgcg
     3001 tgtgctgttc tttgccctgc tgattgcagt cgccgccagc acggccatcg gctacttcag
     3061 cgcccgcctg aacgacggca tgctgctgcg cgccaccgag tttcttgccg ccgacctacg
     3121 cctgaccggc agctcgccca gcgcgcctga acaaatcagc agcggcaagc agctggggct
     3181 ggagcatgcc cagttggtgg agttttccag cgtggtcgcc agcgatgccg gcattcagct
     3241 tgccagcgtc aaagcagcgg acaacgccta tccactacgc ggtgaactga agagtaccgc
     3301 cgcactgtat gacgttgaac aaaccggcgc cggcccgcag cccggtgaag cctgggccga
     3361 agcccgcctg tttgtcgcac tcaacctcaa acccggcgac agcatcgagg tcggcgcgaa
     3421 aaccctgcgc ctgacccgcg tattgacctt cctgccggat gaagccggcg atttctacag
     3481 cctgaccccg cacctgctga tgcacctgga tgacctggcc gccaccggcg tggtgcaacc
     3541 cggcagccgc gtgcgctatc aggaactgtg gcgcggcgag gctgatgcct tggcgcagta
     3601 tcgtcagggc atcacccttg cgcccaacca acgcctggaa gatgccaaag atggcaatcg
     3661 tcaggtcggc agcgccctgg gccgcgccga acgctatctg aacctcgcca gcctggccgc
     3721 cgtgctactg gccggggttg cagtggcctt gtcggcggca cgctttgccg ccaggcgttt
     3781 cgatgccagc gcactgctgc gctgtctggg cctctcacgc aaccaggcgc tcggtctgtt
     3841 cagcctgcaa ctggcgctgc tgggcattac cgccagcctg atcggtgcgt tattgggctg
     3901 gctggcacag ctggcgttat tcaacctgct gcaagaccta ttacccgcgc agatcccgcc
     3961 tggcggcctg tggcccgcgc tggccggcat ggccaccggg ctggtggccc tcgccggctt
     4021 cgccttgccg ccactggccg cgctgggccg cgtaccgccg ctgcgtgtcc tgcgccgcga
     4081 cctgctgccg gtgccggcca gctcctggct ggtgtatggc gcagccttgc tggccctcgg
     4141 cctgatcatg tggcgtttga gtctcgatct acagctgacc tttgccctac tcggcggcgg
     4201 cctggtaacc gcattgctac tgggtggatt gctgctgctc ggtctgcaga gcctgcgccg
     4261 catgctggcc ggtgcgtcac tgccctggcg gctgggcctg ggccaattgc tgcgtcaccc
     4321 actggcggca gccggacagt cgctggcgtt tggcctgatc attctggcca tggcgctaat
     4381 cgccttgcta cgcggcgagc tgctcgatac ctggcaggat cagctgcccg aaaatgcgcc
     4441 aaaccacttc gccctgaata tcctgccggc ggacaaagac tccttcagcg cccgcctgag
     4501 cgagctgtcg ccacaccctg cgccgctatt ccccgtggtg cccggtcgcc taatcatgat
     4561 caatggcgag ccggtacggc agatcgtcag caaggaatcc cagggcgagc gcgcgattcg
     4621 ccgcgaccta agcctgacct gggccgccga gttgccggcg gataacaacc ttattgccgg
     4681 tagctggtgg acagatgcca acgccagcga attgccgggc gtatcggtgg agtccgagct
     4741 ggccgaaagc ctacaattga agctgggcga tcgcctgagc ttcagtgtcg gcggcctcaa
     4801 ccgcgacgcg gtggtcagca gcctgcgtga agtcgactgg aacaacttcc agcccaactt
     4861 ctacatgatc ttccagcccg gcaccttgca ggatctgccg gttacctacc tgaccagctt
     4921 ctacctgccg ccgcagtacg aaaagcaact ggtcgaactg tcgcgcgcct tcccggcggt
     4981 aacgctgctg caggtcgaag cgctgctagc acaactgcgc agcatcctcg cccaggtcac
     5041 cctggccatc gagttcgtcc tgttgttcgt cctggcggcg ggtctggccg tgctgttcgc
     5101 cgggctgcaa gccaccttgg atgagcgcat ccgtcaaggc gctctgctgc gcgctttggg
     5161 cgccgaacgc aaactgctga tcaaggcgcg ccgcgccgag ttcggcctgc tcggcgcatc
     5221 aagcgggctg ctggcggcgt ttggctgtga gctgatcagc ttcctgctct atcgctttgc
     5281 cttcgatcta gcctggcagc cgcatccctg gctgctattg ctgccgttga tcggtgcgct
     5341 gctggtaggc acggctggcg ttctcggtac gcgccgggcg ctaaacatta gcccgctgac
     5401 ggtactgcgc gagggctgat agactcgcgc accactatca acaagctgaa ctatgagtcg
     5461 ctaccgccca ccccgctccg ccggcactgc cctgatcact cccgagggcg aggcccgcat
     5521 gcgcgccgag ctgcatgagc tttggcatgt gcgccgcccc caggttaccc agtcggtcag
     5581 tgaagctgcg gcccagggcg accgctcgga gaacgctgaa tacacctacg gcaagaagat
     5641 gctgcgcgag atcgacagcc gcgtacgctt tctgaccaag cgcctggaaa acctcaaggt
     5701 cgtcagcgcc aggcctagtg acccggataa agtctacttc ggcgcctggg tcaccctgga
     5761 agatgaggac ggcagcgaat cgcgctaccg catcatcggc ccggatgagc tggacctgaa
     5821 aaacaacctg atcagcatcg actcgcccct ggcccgcgcc ctgattggca aaagtctgga
     5881 cgccgaagta cgggtgcaaa ccccgaccgg cgagaagacc tggtacatcg ttgccatcga
     5941 ctacctgtga ccgagccgca catgccctcc gcccctgatg taccaaccca gcaagcccgc
     6001 cttgagtggg atgagcaggg ccaaccgctg tccagccagt tcgccgatgt gtatttctcc
     6061 aacgagaacg ctctggccga aacccgctat gtgtttctcg ccaacaacca gctgccggaa
     6121 cgcttcgccg cgctgacggc cgatcagaag atggtaattg gcgaaaccgg cttcggcacc
     6181 gggctaaatt ttctctgcgc ctggcagctg tttgaacaac aggccgcagc gggcgcgcgc
     6241 ctgcattttg tcagcgtgga aaaatacccg ctgaacaagg ccgatctgca acgcgccttg
     6301 gacctgtggc cggaacttgc gccctacgcc gaacagttgc tggcgcagta cgtggcgctg
     6361 caccctggct ttcagcgact ggtgtttgcc ggcgggcgta tcgtcctgac cctgttgatt
     6421 ggcgatgcgc tgcagctttt cggccaactg gatgcccagg tcgacgcctg gtttctcgac
     6481 ggctttgctc cggcgaaaaa cccggatatg tggacgccgg agctattcgc cgaactggcc
     6541 agactgtccc acgccagcac cactttgggc acctttacca gcaccggcta tgtacgccgc
     6601 cgattgaacg atgccggttt caagatgaag cgcgtgccgg gcctgggaaa aaaatgggaa
     6661 gtgcttaaag gcagctttat cggcacagct gaggcgactg aaaagccctg gtttgctcgc
     6721 ccgccgcagc ctaatggtcc gcgcacagcc attgtgatcg gcgcaggtct ggccggctgc
     6781 gccactgccg ccagcctggc ccagcgcggc tggcagatca ccttgctgga acggcataac
     6841 gctatcgccc aggaggcctc gggcaacccg caaggcgtgc tctacctcaa gctctcagcc
     6901 catcacacca cgctgtcacg cttgatcgtc agcggcttcg gccatacccg caggctgcta
     6961 gagcgtctgc acaaaggcca agactgggat aactgcggcg tgctgcaact ggccttcgac
     7021 gccaaagaag cacaacgcca ggcgcagctt gctgcggcct ttccggccga cctgctgacc
     7081 aacctggaca gaaacgccgc cgaaatcaga gccggcataa ccctgccagc cggtggcctg
     7141 ttctaccccg aggccggctg ggtgcatcca ccggcgctgt gtgaactgct tagccagcag
     7201 ccgactatcc agctgcaact gcatcaggaa gccttggagt tgcgccgtga cggtgatagc
     7261 tggcaagcct ggaatggcga gcaactgctg gccagcgcca gtgtggtggt gctggccagc
     7321 gccgccgaga tcaagggctt taatcaagcc gcgcacttac cgctaaaacg catccgcggg
     7381 cagatcagcc gcctgcccgc gacggcggcc agccgcgcgt taagtacagt ggtgtgcgcc
     7441 gaaggttatg tcgccccggt acgccaggat gaacataccc tcggtgcgag tttcgatttc
     7501 aacagcgacg atctgacgct gaacagcgcc gatcacgcga gcaacctgca tcttctggaa
     7561 gagatatcgc cagagctggc caaccggctt aacgtcgccg cgctgagccc agagcaactg
     7621 cagggccgtg cagcgtttcg ctgcaccagt ccggactatc tgccgattgt cggcccgtta
     7681 gccgacgcag ccgcctttgc cgaggcttat gcggtgctaa gcaaggatgc gcggcaagta
     7741 ccggaaacgc cctgcccctg gctcgacggc ctgtatatca acagcgggca tggctcacgc
     7801 ggcctaatca ccgcgccact gtccggcgag ctgatcgcag cctggctcaa cgatgaaccg
     7861 ctgccagtgc ctgccgatgt ggcgcaggcc tgccatccga atcgcttttc cttgcgcgca
     7921 ctgatccgca acaaatcctg aaagccacgc gcctgcgaac aggcgcgcaa gcatcaacca
     7981 cgtaggctaa tacgccaagc gcggtggatc ttctggttgc gagcaaagtc cggatccagg
     8041 gtcgattcgc tgatttcctc aacccgataa cgcgcggcca ggctctcatc gagcacaaac
     8101 ttgcggaagt tgttggagaa atacagcacg ccgcccggtg ccagacgcgc cattgccagg
     8161 tcgagcatct gcacgtgatc acgctggatg tcgaagatgc cttccatgcg cttggagttg
     8221 gagaaagtcg gcggatcaat aaagatcagc tcgtactcgc cacggtcttc ggccagccag
     8281 gtcatcacgt cgccctgctc cagcttgttc ttgtccgaat aaccgttgag cgacaggttg
     8341 cggcgcgccc agtccagata ggttttcgac aggtcgacac tggtggtggt acgcgcgccg
     8401 cccttggccg catgcacggt cgcggtggcg gtgtagcaga acaggttgag gaagcgcttg
     8461 ccggcggcct ctttctgaat acgcagacgc agcggacggt gatcgaggaa caggccggtg
     8521 tccaggtaat cggtgaggtt gaccagcagc tttacgccgc cctcgctgac ttccataaac
     8581 tggccttggg tggcctggcg ctggtactgc ttggtgccgc tctggcgctc gcggcgcttg
     8641 atcaccacct tgttcttgtc gacacctagc gcctgtggaa tcgcggcgat ggcatcaaac
     8701 atgcgctctt tggctttctc aggatcaatg gacttgggtg cggcatattc ctgcacatgc
     8761 acccagtcac catacaaatc caccgccatg gaatattccg gcatatcggc gtcgtacagg
     8821 cggtagcact caacgccttc cttgcgcgcc cacttgccca gttgcttgag gttcttctgc
     8881 aggcggttgg caaacatctg cccgccttca ctcaggcgcg cctgctcaac caccggagca
     8941 ggtgccggag tcggtttgat cgggttgccg ttcttgttgt actgacgctc cagcggctca
     9001 agcggggatt tgtccgcttc ggcctgctcg cgttcacgtt cgcgctgctc aggggtacga
     9061 cgctcgccgg tgacgaactg ctcgggcagc accttgatca gcagcagctt gcaaggcagt
     9121 gcgccattcc agaaagcgta ctgcttgtgg ctacggatac ccatgcgctt gcccaggtcc
     9181 ggcgcgccgg taaacaccgc cgcttcccag cccatgcaag cctgacgcag acgctcgccg
     9241 aggttctggt agagatacag caagctggct tcatcaccca ggcgctcgcc gtagggtgga
     9301 ttgcagatca ccaggccttt ctggttctga tccggacgcg gctcgaaggt cgccacttcc
     9361 ccctgataga ccttgatcca atcgctcatg cctgcgcgtt cgatgttatt gcgcgcgggc
     9421 tgaatcaacc gtgggtcggc ttcataaccg cgaatccata gagggggttt agccatacct
     9481 gctgcggcgc gctgctcggc ctcggcatgc actttcttcc acaaggctgg cacgtggccc
     9541 agccacttgc tgaagcccca ttgctcgcgg gtcatattcg gcgcaatgtc ggcagcgatc
     9601 atcgccgcct cgaccaggaa ggtgccgaca ccacacattg ggtcagccag tgcgccgcct
     9661 tcggcagcaa tacgtggcca gccggcgcgg atcagtacgg cagcggcaag gttttccttc
     9721 agcggtgcag cgccctgctg caggcgataa ccgcgctgat gcaggctatg gccggacagg
     9781 tcgagggaga gaatcgcctc accgcgatcc aggcgcagat gaatgcgcag gtccggattt
     9841 agcttgtcca ccgacggccg cgtaccgtct ttcaggcgca gcttgtcgac gatggcatcc
     9901 ttgaccttca acgcaccgaa atgggtgttg tcgatgcccg agccgttacc gctgaactcc
     9961 acggccaggc tgccggtgga ttccaggtgc tcgaaccagt cgacctccag cacaccttcg
    10021 tagaggctct gggcgtcctg cacgctgtag cgcttgagta ccaaaagcac gcggttggcc
    10081 aggcgcgacc acaggcaaag acggtaggcg gtttccatgt cggccatgcc acggatggcc
    10141 gaggtgtgct cacgcgtgtc ctcaaggccg aggctgctgg cttcttcgag cagcaggcct
    10201 tcgaggccct tggggcaagt gaggaagagt tcgtaacgat ccgacatggg agtttccagt
    10261 gcctaaggca atcaacggcg cgcagacaaa tcagcacggc caaaataagt gacaaagcgt
    10321 cgcagcgcga cccttcgtcg gaataaacaa cagtctcaat cgcaattcat tcgcgatagc
    10381 aaaacaccag cacacccggc gcatacgccc acagcatagg cgctacagcg aaaactccgg
    10441 gcgctcgccc taacgcctta tggcctgacc ctcaaaaagg ttacggcctt atgacgaaac
    10501 ggtcattcac aggcacccgc gcattcgtta gaaatctacc taggccagca ctgcaacggt
    10561 gctggcaagg aggctcgcga cgccggcagc ggactccaag gcagaacttt ctgcctgacc
    10621 ttgctacaag gtctaaggga cataacagtc aacattgagg gcttcaccct atgagaagac
    10681 ttaagcgtga tccgttagaa agagcttttt tgcgcggcta tcaatacggt atcagtggta
    10741 aatcccgcga actctgtcca ttcaccctcc ccactgtccg ccaagcctgg ttaaacggct
    10801 ggcgcgaagg ccgcggtgac aattgggacg gcatgaccgg caccgccggc atccatcgac
    10861 ttaacgaact tcacgctgtc ggctgattca ggaacctacc gactcaccat gcgcgctcca
    10921 tccgagcggc gggcgcaagc ccaggggctc ctttaaggag ccctttttat tggccctgat
    10981 atttttgcgg aagagccgca atggcatcca cagcttcgcg aatcaatgca gggcccttgt
    11041 agataaaacc ggagtaaatc tgtaccaggc tggcaccggc agcgatcttc tcagccgcat
    11101 gcttgccttc ggtaatgccg cccacagcga tgatcggcaa acggcctttc aactcaccgg
    11161 ccaacacctg aacaatgtgc gtgctcttct cacacaccgg tgcaccggac aatccacccg
    11221 cctcgtcggc acactccaga ccatgcacac cttcgcgacc cagcgtggtg ttggtggcga
    11281 tcaccgcatc catgccggct tcaaccagcg cattggccac ttcgatggtt tcgtcgtcgc
    11341 tcatgtctgg cgcgatcttg atcgccagcg gcacacgctt gccgtactgc gcagccaaat
    11401 cttcctgacg cttgcgcagg gcttcgagca attgcttgag cgagtcacca aactgcaggc
    11461 tgcgcagccc tggggtgttc ggcgagctga cgttaacggt gacgtagctg gcgtgctgat
    11521 agaccttatc caggcacagc aggtagtcat ccactgcatt ctcgaccggt gtatcgaagt
    11581 tcttgccgat attgatgccc agcacgccct tgtatttagc cgcctgcacg cgggccagca
    11641 ggtgatctac gccgtggttg ttgaagccca tgcgattgat gatcgcctca gcctcgggca
    11701 agcggaacag gcgcggcttg gggttacccg gctgcggacg tggcgtcacg gtgccaattt
    11761 ccacaaaacc gaagcctaac tgagcaaaac catcgatggc gtcgccgttc ttgtccaagc
    11821 cggcagccaa accgaccgga ttgggaaact ccagccccat caccttgacc ggcagcgcgg
    11881 ccgggctctt ggtcagtaag ccgttaagcc ccagacgacc gccggcaccg atcaagtcga
    11941 tggacagttc atgggaggct tcgggggaca gtttgaacag cagctcgcgg gccagggtat
    12001 acatgggcag gcttagctcg ataaagttac aaaggccgcg attatagccg gctgcgcacc
    12061 tgataggcga ggcgcacggc gctatcagcc taagcggcgc aggctgagta actcaccaac
    12121 gggactgaac tcgagctcga aggaaccata gaccccgtca tgggtcagaa acggccgaaa
    12181 atggtgggcc ggcacactga cactccgccc atcacgactt tgcactaata tccggttggc
    12241 acgcccctga tagaccacac gcaagcgctc ggccgataac gcaatatcaa acaccaaact
    12301 gggcatggag gcacctttgc aactaacagc agcaatcttg ccatacgccg cgcgtatgcc
    12361 cggtaaaaaa cctgtcgcca cggcagataa agcctgtgca gggccgcgtg ggctctgcca
    12421 aactggcaat ggtgattttg gagttcgtac ctggctatga gtctgtctga gcagccttcc
    12481 accctcagct cacgcctcgg tgccttagcg caaaggctag gccttatcgg ccgtgggcaa
    12541 cgtcagatac tcgagcgcgc tagccaactt cgcctgccct tcagccccct gccacaactg
    12601 cgtaacagta tctgctggca agatggcccc cagctacacc gcctgataga cctgccccgc
    12661 agcgcactct ccggcccagt tcaggaagac aaggcgcaag cccacgccat gctggtgcgg
    12721 gtgatcgaag cacaagaaca gcagctgaca tcgttcgatc tgcgcatgat cgacggccta
    12781 tcgacctgca accctcagca ggccaaccac accagcttcg aggtcttcgc cgccagcgaa
    12841 acctgccgcc aggtacgcat catcagttac aaggacttcg tcaaagccat cagccaggcg
    12901 ctaccgcgtt tcctcgctgg cgaacgcatt atcctgcgcc aagccagctg gcgtggcgag
    12961 cgggtattct gggcaggcga gcatcatagt gaagcttttg cttgcgccat agcttacgcg
    13021 cgtaggcgcg agctagaggt caacttgccg gccgaactca gccattaccg cttgagcgag
    13081 caaggcctca gcaacctgca gcagcactac cacgtactgg gcatgcccaa tgcggcctgg
    13141 agcgaccctg cgtttatggc gctgctgctg gataacggcc tgccctatgc gcgcctatcc
    13201 ttgctaggcg cgccaggctc accggagttt ctcctgctac ccaaacactc cagcgaagcc
    13261 acagcactgg gtgaaggtct gcgcctcgcg ggtgcgccgg atgtggtggg gtatctgcaa
    13321 cagctgtaac cgctacatcg ccgccatcca atgagtcctc aggaaaccaa gtaagaaaaa
    13381 gcccgtaatc tgattaggat tgcgggcttc agtatgacta ccaatctaac ttcacactac
    13441 ctgcaaggcg ttatctccca acagcccatt gatgacactg gctgtatccg taccaccaga
    13501 aatgatgccg ccaccgcccg gagtcacacc gtactcaacc gcgacattaa agccagtcaa
    13561 ctcaacggtt gtggttgcaa cgccgcctgc tgtagggctc accgcaataa tggtgctgtt
    13621 atcaactacc gagaagctga ggaagtcatc tagcgatcca gcgttgtagg actcaccaat
    13681 cagcaactgg gtaagatcca acttgtcacc ttcactcggg ttgaagtcga ggaccttgtc
    13741 ggcacccgtg atatcggtgg acagccaacg gaagatgtcc gctccggcac cgccggccag
    13801 aacatcgtca cccaagcctc cgatgagaat gtcatcaccc gcaccgccgg agaggatgtc
    13861 gtagccttca agaccgctga gcgtgtcatt gccaccgttg ccgaaaaggt aatctgcctg
    13921 agcggtcccc tccaggttgt caacgccagc gccgccatcc accgatagcg agtcgggtac
    13981 cagcgttgcc ttcagcgtgc tggagacact gtcaccgtcg ccatcgatac cgatgatgtt
    14041 gtgcgagaga ttaataggtt gcccggttga agcctggtcg tagttgaaga agcccagttt
    14101 gaacggctgc tcattatcac cttcgatctg caccgcatta aatgcagttg tagagttcac
    14161 ttcataccac cagcccttct ccatcccgga aatacgaatc caattaccag tatcctgaat
    14221 cgtaacttca ctagctttgt ttacctcaac gccattctcg attgcaaaga tacgtatgtt
    14281 gtttgtagat agatttatgt aggcatctcc agaatcacca ataaaactgt aatcattgtc
    14341 ggccaacagc actgaaatcc agaacgctgc agtgccacta ccgctgggat tgacagaatc
    14401 aaactgctgc gcgaaacggt ttgtggtgtt gtgactatcg tagaggaaac cagtgccatt
    14461 gctgcctccc tgcctcagcc cgtttactaa atcgatacgt aatctttctc catcactgaa
    14521 gctgttaccc gtacctatac caacctcggt gctattacta ttgacagtct gccccacagt
    14581 agtagaaagc agtgcatcaa acgtgctgcc cccaacgtcg ataagcccct taaagggcac
    14641 attgccacca ccaacaccgg tcagattggt cgcagacacc tcactgccat tggcgatggg
    14701 gccgtaggtc gtgagcgtat agctatccgt ggcggcatcc atgtcgatac ggtaccccag
    14761 gttgccgttg gcactggtct tagcctcgat gatcgactga tcactgccgt agaacagata
    14821 caactgctgg ccgcccatgc ttagcaggtt accgctggca tcccgagccg ccgcgccatt
    14881 gacgatggag aagatcacct ggtcggggcc atcggcacct actgaagcct cgaaacgcag
    14941 gtcagctgtt atggagcggg tttcactgac ctgatcgacc agcaacgccg tgaccggagt
    15001 aaaggcaatg ggtacatcgt cctcaacctg gatcagcaga tcgccggcaa tcggtaacgg
    15061 attcacacca ttactggcct tgattgcatt ggtcagattc agcgtgagaa tttcgttgtc
    15121 gttagtgccc gcgctagggt gatccagtgg gccaagcagc gtgaaagtat aagtaccgtc
    15181 gctcatcacc ttgagggtga acacatcagt actaccggct ttggcggtca aggtgtcacc
    15241 agagaagcta tacgtaatcg cgacaccacc agaggttatt gtcggcatga tcgccggtga
    15301 ggtattcagg ctgaactgcc cattattggt acccaccaac aaatcaacca ggctaccggt
    15361 agcgactgtg tttacgctgt caccatccgt tatgcctcca ggcagctcgt cctcgtccac
    15421 tcggccgata atcggagagg cgtcctcaat attaatggtc agagaagcgc tggaagggtc
    15481 gccatccttg tcaacgatgg tgtagccgaa gctctcgctc gtatcagcag ccacactcgg
    15541 cggcgccatg tagctccagg ctcccgtggc gaagttgaaa ctcaatttac cgcccagacc
    15601 tgtggcgatt tccgtcagct gattaccggc aatattgcca ccggtactga cgctaatagt
    15661 gccgacgcca ttccaggtgt aagtcgcccc gttgatgaca atcgattgga tatagcctgg
    15721 gccatcggca ccgaagctat catcgtcacc gccgcccact gcattatcgc tacccagcag
    15781 tacattgccg ctgacgccgc cgccgaccag ctgactcagc agagtgtcaa tcagaccatt
    15841 gaagttactg atcaggatgg gtgaaccatt gccatcgaca tccacatctt gcaggcgagc
    15901 agcattgatg ccgtcgccaa caccgatggt cgtgacattg atgccgctat tgtcgacaaa
    15961 actgttccag gccgtagcag tacttgtggt cagcgaggag tcacttgggt cggtttgctg
    16021 atttggatta ccgtcactga tgaaaaatac ctgattgctc caactaggta tcgggctgta
    16081 aaccgccatg gtttcctcaa tggcatcagt gaaatcggtc tgcccactga acggccgatt
    16141 ggcaatgatg ttgtcaatca gggcgttgaa agacgccaac gtagtgaccg ctggataggc
    16201 cagcgaagtc gatgagaacg ccactacagt aaccttcaca tcaccgtcag taccggtata
    16261 cagcgcttgt cctgccgctt tcacggcatt catcatggtg ctgaactcgc tattgctgat
    16321 actgccgctg gagtccaata caaaggctac gttgaagtct tgcccgcgtc cttccgccac
    16381 atcggcggta tcgttcttag ccagcggtgt atcgtcgtca acgttgattg acaacgtgcc
    16441 tgtggccttg tcgccatctt tatcagtcac ctgataactc aggttaaagg cctggttgtt
    16501 ctcctgattg gcattggcgt gttggatcgg tgcgttctga gtaacggtgt agacaccagt
    16561 gccggtgttc agcgttaacg tcagcactgt agtagcaccc tgcttaacca gcagttgcga
    16621 gccatccggg cttgcctggt aggtgaagcc gcccggcgct ccggactcca gccatttcac
    16681 cgtaccggca ccgtcagcac cgaagttgtg ctgcaaagtg ccaccggcat tcaccgagtc
    16741 ggcatcgtcg gtaccgccgg ggccaccagc tatgccattc gccagcgcat catcatccaa
    16801 ttgcacgggt gcattggcac tgacggttgg cacatcgtct tcaatctgta ccaggaagct
    16861 gcccgccagt ggcacagggt taatcccgtt gctggcctgc aaaatactgg cgaaattaat
    16921 caccagcagt tcgtcgtcat caccgttgcc cagtggatgg tcgagtggtt tgagtaaggt
    16981 aaaggtgtag tcaccgtcac tttccacctg cagggtgaag acggtatccg tacccgccat
    17041 ggcgctcaac gtatcgccaa ccacgctgta caccagagga gtcccaccgg atgaggcgcc
    17101 atccaaaccg ctggtattac cagacagact gaacagcacg ccgacagatg gcccgaccac
    17161 taagttacta acactgccgg tagcgatagt agtttcccca tcgctatcag ttataccgcc
    17221 aggcaattcg tcttcaacaa ctttggcaat gactggactt atgtcctcca catacacagt
    17281 caaagtcgct tcagacggat caccatcctt atcgacgatg acatagctga aggtttcaac
    17341 cttgtcgccg ccaccactac ttggagcctt atagatccag gcacctgtgg cgaagttgaa
    17401 atccaactta ccgccatggg tggtggggat gttgctcagt atctggccgc catccagcac
    17461 accatcccaa gtgtgggtaa tgccattgat ctcaatggac atgatccggc cggggccatc
    17521 ggcgccatag ccatcatcgt caccagcgcc gccattatcg acaccgtcgc tgccatgcag
    17581 cacgttgcca ttgaccagac caccaaccac gttatcaagc agcgtgtcga ccaggtcgtc
    17641 aaagtcatct gctcgcagcg gatcgttgtt cggccctgca tccatatcaa cgttttctag
    17701 ccgctgatca atgatgccgt tgcctacgcc gatggtagtg acagtgattc cgttagtgtc
    17761 gacaaaggtg ttccacaccg ccgcagttgt atcagtcagc gaatggcctc cagtacctgt
    17821 ctgctcattg gggttgccgt cactgataaa tacgacctga ttgttccagc ctggctgtgg
    17881 cacaaaggaa accatagtct gctggatagc atcggtgaag tcagtggtgt tatccagtgg
    17941 ccgcacgcca cccggctctg aggggtttaa ggagttcact aagttggtaa acgtagtgat
    18001 gtcgctgctt accggataac cgatcgaatc gtgcgaaaac gccacgatct gaatctgcac
    18061 cgcaccggca gcattgttga acagctcctg accggccgtg cgcacagcat ccagcatcag
    18121 attcagctcg gtattatcaa tgctgccgct gaagtcgaga acgaacacca tattgatgtt
    18181 ctcggtctga ccggtgctga cataggccac atcgtcctga gccaccgggg tatcgtcatc
    18241 tacattgatg gccaaagtcc cgtcggctgt atctccatct ttatcggtaa cccgataatt
    18301 gaggataaag gcctgattat tctcttgatc agcctcggca tgcaggattg gtgcgttctg
    18361 cgtaacggtg taggcaccgc tagcactgtt cagtgtcaca gtcagcacta gagtggtgcc
    18421 ctgactaatc aacaacgaac cgccagggcc tgccgcgtag gtgaagtctg ccggtgcacc
    18481 gctagtaagc cactgcacat taccggcacc gtcagcgccg aagttcaggc ccagcgtacc
    18541 gctgatttcc acagcattgt cgtcatcacc tattccgccc ggaataccat tggccagggc
    18601 atcgtcgtcc agttgcacca gggcatttgg caacacactt gggccatcat ccttgaagct
    18661 gatgaccgtg ccgatatcaa tgcttgcact ggcctggtca ccatccccat cggtcacgac
    18721 ggcattcaag ctgacatgcc ccgcacccag gaacttggct tcatctggat cgcccgttgg
    18781 gtgaacaatg gcgcgaatct ggtcaagagt gacggtgcct gtcgcatcga cactcagggt
    18841 aaataccaat tggtttgtgc cagaggtacg accctcaatg accccagcat tcaagctaag
    18901 gatcaccgcc tggttggttt cggtatcgac caagcccgag ctggcgtttt gcacactcag
    18961 gttataggac aggggggtca gggcgtgcag gccatcagca cccggctgca cattgaatac
    19021 gccgctgaag ttagccgttg catcagcgct gaaatcagat tcatcgacct gcagcgatga
    19081 gccccaagtg acattggtgc tgacatcgtt gtagtcgtaa tccgaggtaa gtgtttggtc
    19141 ctcccagttc tgattgcccg cgttgccggt gtcctgcaaa tgcgagccgc cagggttcag
    19201 gctggcatca ctgaacagca cattggcacc gtcagcaccc actagtggta cgccacccaa
    19261 cttcgcctgc cactggccgg cgacaaactc gaaggtcaca accgagccat tagtcaggtc
    19321 tgcattggct ccgccattcg ggatgataaa gaagccggta ttggctggat ccaggccact
    19381 gagatcagcc acatcgccaa cggattgagc atgcacattg gcccagatca ctttgccgtc
    19441 gactggtgaa ccgtctggcg ccttgttgta gtagccatag gagttgctga aaccagcatc
    19501 ggtgccctgg aaggtgacaa aggaagtcac gccaatattc ccggcctgaa tagtcggagt
    19561 atcgtcatca atcgtcacga ccaacgaacc cgtggccgag tcgttatcac cgtcagtcac
    19621 cgtgtaaccg atgttcagag tgatatcgtc ttcggtgccg ctgaccgggt gcatcaatgg
    19681 ttgcagcaag ttgatggtgt aggcgccgct ggctgggtcg gtaagcacca ctgtcagcaa
    19741 tgccccgcgg acagagctga tggtcagggt attggttggg gcatcccagt tagatgtcac
    19801 agcctccgag cccaagctta tcggcccgct gagcacaatg gatttgaggc catcagcacc
    19861 ggcattaaat gccaaattcc cactggtgct ggtagcagcg ccggcaacat cgcctgggcc
    19921 accatttatg ccgccaagca gaccctcatc atccagaaca accgctgccg cagtcttgcc
    19981 agccaccggg ccatcgtctt caaagctgaa gacacccgat gcacccaggt caatcgaggc
    20041 gctgtcgctg tcgccatcac cgtcggttac ggtctgggtc agcagcagtg caccttcagc
    20101 cagggccagc acttcgttgt catcggcatc ggcggtgtcg tcgtgccaga ggttatccag
    20161 cagctccagg gtcacttcac cactgaccgg gtcgatggcc agggtgaaat acggcacgcc
    20221 accggcgctg ccggtaatca cgttgcccag ctggttcagg acgatctgtt cgcctttgcc
    20281 caacggggca ctggcgtcca ccgcaaacag gccactcggt acatccgcac cgctcagctg
    20341 caggctgtac accgtgccgt catcgccggc tgccgca
//
LOCUS       DASRUM010000268        22454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2155962, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000268 DASRUM010000000
VERSION     DASRUM010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 22454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22454
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2155962"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..8557)
                     /locus_tag="VFF22_18120"
     CDS             complement(<1..8557)
                     /locus_tag="VFF22_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003200896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HZX18772.1"
                     /translation="MSNIEDIYSLSPTQHGLLFHSVYEPDSRVYYQQLSLDMTGPLDT
                     GAFRRAWQALMQRHAVLRTAFLWEDLDDAYQVVQHEVPLPLSELDWRDHNEEAEDALR
                     QRARLERDQALELNEAPLMRLCLVSLPEQRWHLIWTFHHILMDGWSVGVAIQEWLALY
                     YEQLRGRAFSLPPSRGYRDYIAWLGEQDADATEGFWREQLQEVSEPTPLPSFAPPPEA
                     PQEHPYAERETRLDAAETAHLTQFARQHHLTVNTLIQGAWALLLGQHAGRDEVVYGVT
                     VAGRPDALASVDSMVGLFINTLPLRVQLTDERPLSDWLQALQQRNSDLRQHAYLPLSR
                     LKPLSQVPAERALFDSILVFENFPVTDALNQDADGLRFSAPPELRRDDGIKLTHGRNH
                     FPLSLIVVPGERLEYLFSYERSRFSDGEIATLSAQLRAILLAMAEQPQARVQALDWLS
                     DDERQRVQAYGQGAHLEVAAETLHQRFAHWAATSPEQLAVRDCQRALNYRELDAEANR
                     LAHHLQAQGIGAGRLVALALERSVDFVIALLATLKSGAAYLPLDLKQPAGRLADILAD
                     SRADLLISRRPLESRLPRDEAPARLWLDDQAAAIAALPSSAAPLAHDPAAPAYVIYTS
                     GSTGIPKGVLVEHRAIVGYLAAVAQLLDPPPAARYALLSTLAADLGHTQLFGALCSGG
                     SLLLVDEDTSFDPLALADFFGEHPVDVLKITPGHLHGLLQAHPDPRLLPRARLVFGGD
                     ALKPALLEQVRALAPTLQLFNHYGPSEATVGAVATALQATVGLVPLGRPLANRQLRVV
                     DNSGRSAATGVPGELWIAGDLARGYLHHPQLSAERFVDADQQRWYRTGDRVRWLNDGQ
                     LAFLGRVDNQVKIRGNRIELGEVEAQIKRLSPLIDQVLVRVLDVDGTQRLVAYVVARQ
                     SLSANKLCEDLGRRVPDYMVPAHVITLDAIPLTANGKPDLRQLPLPSAPVDDGASHVA
                     PRNEVETLLARIWQDVLKVERVGVHDNFFALGGDSILNLQIIARANQQGLKLTPKQLF
                     DNRTIADIAQVLGSGAETAVARQQSEGFAIALTAGQRARLEAGEANASWRCIALNGQV
                     PSAQLAQALAALQSHHQALRLALRQTPDGDWQQQVLAAPVPCAVDEQPQVDVSPAALQ
                     TLADNAFARLDLSVGQSLHATLLRCAEGDQLLLVAHPLCLDEAAWPLLLADLNLALSQ
                     LRHQRPLRLSYKGGELSAWSEQQQRDASSDRLDEVWEHWLQYAGLDLPPLELSASSQT
                     VEQRLDAANSARLKRLIETLQLDWNTLLTAALVEQLRTVEHAPLALTLLAGRPEVERL
                     PLSAPIAAADLDPARLLGPLALPVPFYLQAPGGNALERLQAVAAQLRAYPQHGADYGT
                     LRYLADNPYLLEPLRALPAAPVTLRWLGDWDAHREAHAVLDEIRAASREAAGASALTL
                     DACWHNGQLQLHGTGTLAASWTPALIAHLHTLAELADSPALRPASEAFPLCHEQAATL
                     ASEPLPWDRLQDLYPLSPMQQGMLLHTLLQPHSGIYLMQQRYSWDGELQRPAMEQAWR
                     LFLERHPMLRTAFSWQDDHAPLQCLYRDTELAFDWYDWRHLDAEQQREAMDRMLADER
                     QRGFDMSRAPLTHLRVFQLAEQRFSMVRSFHHILTDAWCFGLLMEDLLAIYQAVARQE
                     PVARPHFRSFRGYMSWLTQQDMASAQNFWSNELAGFEEPTPLQVDIPVAQPDRAPEAV
                     GDCNLTLSVTRTQRLQQLCQQHQLTPNTWIQGAWALLLSRYSGNRDVLFGVTVAGRPT
                     SLAGVEEMVGLFINSLPLRIDVDPEQPVIGWLQALLSHNAELRDYEFAPLVDIQRWSE
                     IPRGRQLFDSLVVFENAPLDISSVKLDSFSIDIYEDRVHTNFPMTVVLYPGDRLGIRL
                     SYDQGRFAADTVQRMLGHLVQLLGEMLERPDAALGTLQMLPAAERQQLLVDWNQSAAD
                     FPLDRGYAALFAEQVQAHPQRLAAVCAGESLSYAELDRRANAIAHALQQAGAGPDTLV
                     ALAAERGLALLTMMIATLKAGAAFQALDIQHPPQRLAELLELSGAPLLLLDEKASGLL
                     DAVLPGVTRQPTCLVVQQLWHGEGAAVTYPHQADQLAYVIFTSGSTGTPKGALVEQRG
                     MLNNIFGKLPTLDLNAEDRIAQTASPAFDISVWQFLAAPLLGATVHILPDAVAHDPAA
                     LLDAIEQQRLTLLEAVPALIRGLLQEARPQHQLTSLRWLLPTGEALPPALARDWRQRF
                     PATPLMNAYGPAECSDDVAFHPIHEAPQDDCAHMPIGRPTANNQLFILDTAMHPVPIG
                     VPGELCVGGVGVGRGYLNDAERTRAAFVDHPFQPGARFYRTGDVGRYRADGVIEYLGR
                     HDQQVKIRGHRIELGEIENRLMRHPLVDSAAVLALPDARGDLQLLACYVLAHEHGATV
                     DDATARASLIEHLRPQLAAYMVPGHWQRLEQLPLNANGKVDRRALTALGLPSEDAPQA
                     LVAPRTPTEQALAELWQTLLGLTSLSVHDDFFALGGHSLLATQVISRIRRRLNVDLPL
                     RALFEHRTVAELAQVVDAQVPTKQASEEIPRASRAQPLPLSYAQQRLWFLDRLEGASA
                     AYNLSTAVRLSGDLDVQALRAALQHVVERHESLRTGFSLEGDTPRQVITDDVELELAL
                     QHLDANDDTQLQQYIDEEAARPFDLQRPPMLRARLLRLSDRQHVLQFTLHHIATDAWS
                     MGILVEEAITAYRAYSLGETPNLAALPIQYADYAHWQRSAPQQQRLARDIDYWRGQLQ
                     GAPMLLRLPLDRPRPASPNYQGAAIRQTLTLAQTERLKSYAQQQRATLFMVLLNAFNC
                     LLQRATGEDDFLVGTDLANREHPA"
     gene            complement(8648..22444)
                     /locus_tag="VFF22_18125"
     CDS             complement(8648..22444)
                     /locus_tag="VFF22_18125"
                     /inference="COORDINATES: protein motif:HMM:NF004282.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthase/polyketide
                     synthase"
                     /protein_id="HZX18773.1"
                     /translation="MDLEGLSEAQQEAHIQALLEQEQRNGFNLAKPPLIRVRLVRLGA
                     GRHEMIRSFHHILMDAWCISLIMVDCIDAYDALRQGRTPTMRKPRPYRDYIAWLQKQS
                     LPEAQAFWQQQLQGLDTPTPLVIERQTRQSQYSEAVLVDDTLIRFDARETRELTALCQ
                     NQRITPNTLFQGAWALLLSRYSGHRDVLFGVTVAGRPPQLAGVEEMIGLFINTLPLRV
                     QIDEQQDRIGWLQALQRYNLGLREFEHSALVDIQGWSAFPRGEELFDSILVYENAPID
                     DKLFSGELSFSLDDMEHSVHTHYGLTVVVMPGEQLAVRISYDRRRFTRDSIERLLGHL
                     RQIVLGILAAPSASLASLELLSDAEHQQMLVEWNRTQQPFPLERTYASLFAEQVARQP
                     SKVAVVCEGEQLSYAELDERANRLAAALITAGTGPNQPVALLAERGLAHLTALISIFK
                     AGACYLPLEVKHPAQRLAEILELSRAPLMLVGDSQCALADELCTRFGAEAPRVIGIEP
                     LWLSGPAPLIEPRGTADDLAYVIFTSGSTGTPKGALVEQRGMLNNIYGKVPSLGLSEQ
                     DRIAQTASVAFDISVWQLLAAPLLGATVHMLPDAVSHDPERLLEVLQNQQLTLWEAVP
                     AVIRGVLTASTATTDLSALRWLLPTGEALPPALCRDWFARFTHVPLMNAYGPAECADD
                     VAFHAIHAASDEQAQAMPIGRPTPNTELYVLDAELRPLPVGVPGEICVAGAGVGRGYL
                     NDAERTRAAFVDHPFQPGARFYRTGDLGRYRADGVIEFLGRRDQQVKIRGHRIELGEI
                     EAKLQQHPAVKAAAVVVHKDARNESQLVGYWVGHTAEAVDALTLKEALSRQLPAYMVP
                     LRLVELDALPLNANGKVDRKRLVLRELQWSESESTPPANETEQALHGIWQEVLALESF
                     GVTDSFFALGGHSLLATQMLSKIRAVFRIELPLKALFECPSVRQLAQAIDQQLAATSD
                     GPADADAIAVQPRPPRLPLSFAQQRLWFVEQLNPGSAHFNIPFALRLSGELNIEALRA
                     SFQWLLERHEVLRTAFPSEDGQPYQRIVEALSFELPISHLTDVSDATLQTELSSWFAQ
                     PFDLVSPPLIRARLLHLGERQHVLAITLHHLVSDAWSATLALRELTQFYAALQAGETP
                     RLAPLPIQYADYSLWQQQRLQPAALQPHLDYWEHQLARNDGADQDYLLELPTDKPRPA
                     SQSHRSGMLAAQLSPMASERARALAARLQQSPFSLVFAAFASFLQRMSGQDDLILGTP
                     VSNRERSETQPLVGILLNNLPVRVRFQGQPSFAELARQVSDTLLDAQRHQDLPFERLV
                     DHLALPRRLSHAPLFQIMVAQQLPMERRVQFPGLEFEVLDTPLSHSEYDLDLHIVTPS
                     QGPIELNLLYAHDLFEAPSAERLLQRFVHLFEAMLEAPERPLGEHRLLTPDEWQRTVE
                     GWNRTAQALPLAPTILNAFEAQVVRSADALAIEHREHRLTYGELDTQANRLAHHLIAQ
                     GVGRGHWVALCLDKSPELAVAILAVLKCGAAYVPIDTSYPAERIRYILDDCGSDLLLT
                     HGQYAARYTELPVKLLQLDRLQAQLEQQPATPPTRCLDADSPFYAIYTSGSTGQPKGA
                     VVTHGGVRNLQHWYLRTLGWRQGDKVLLASATGFDLTQKNIFAPLLCGATLVMPSVES
                     YDPQALLHDIRSHRITLLNGAPSAFYPLVEIADAQQWRDLASLRALVLGGEPIRMERL
                     HGWLASEHGRCRVINSYGPTECTDVVAWHEVDIDAPNEVLPIGRPVDNNQLYVLDRYD
                     QPLPVGAIGEICVAGAGVGSGYLHREALTASVFVTNPFGEGRLYRTGDLGRYLANGEL
                     EFLGRKDFQIKLRGLRIEPGEIEQALRRLDGVRDSLALVRDDRLIAYVLTATGKLPSE
                     GWRDELGRFIPAYMLPQALVALSEWPLSANGKIDRRALPEPLLEEAGETLEAPQGDSE
                     VRLASLWAETLQVPLEQIGRQSNFFHLGGHSLLATVLLTRIAALFDTAPALRSLFEQP
                     TLAAQARSIDGTTAQPNHTLPTLVAGPHPARLPLSFEQQRLWFLEQLAPDTGEYSIIT
                     ALTLTGELRLEILRQAFETLVERHTILRSRLLDKGDGAELLIDAPAFFNLVVEDYQGS
                     AAAWPEHQRQVAEAEARQPFDLAREHPLRVHLIRHPASGQALLLLGLHHSAADGWSLQ
                     VLLEELAEGYRAGLEKRRPDLPTLPVQYVDYAVWQRSAARQQIYRQQLDYWQTTLENG
                     DYRLELPTDLPRPATFDNTAGTCFARLPQALTTRLRQFAHEQNVTPFMLLLAGLKLFL
                     SRYSGQQDMRIGTPVANRRLDVVQPLIGCFVNTLVIRSELTPQQTFGDYLAQLRQTVL
                     MAQEHQDVPFEQVVEHLAPERDLSRTPLFQVAFVMQNSERAPLQWPDLSLQPLDIRSS
                     AAKFEQNWEVHDDGQQLSVMLEYRACLFHEATMQAWLVQWQDFLGQLPDAVNTPLAQL
                     SPLSEADRQRQLLTWNDTARHYPGLATLSEALAQQALRSPDAPALVCEGETLTYQALH
                     QRADHLAQALAAQGIGREQVVAVCLERCSDLLVALLAIIKAGAAYLPLDPHLPPARLA
                     FMLDDAACPLVLTQSQWRDRLAAQVPAWCLDQPLPEIVGTPRIVANQPGDLAYVIYTS
                     GSTGQPKGVLNQHGALMNRLHWMQEAFPLDATDRVLQKTPYGFDVSVWEFFWPLITGA
                     CLVLAKPGGHQDSGYLGELIQRERISTLHFVPSMLQAFVSEPDLEHCTSLRQVFASGE
                     ALPLALQQRFCTRHPARLINLYGPTEAAIDVSVWICDDASTLGFVPIGKPIANIQLYI
                     LDEGLNPLPLGAVGELYIGGAGLARGYLNRPELTATAFIASPFKAGERLYRTGDRCRF
                     LADGNIQYLGRLDHQVKLRGQRIELGEIDAALLAQPCVREAATLLRDDLGTPQLVAYV
                     VASASGLDVEQLQTALTQALPAHMVPSVVVELAALPLNSNGKLDRKQLPKPHLELSGD
                     TFEAPQGEAEQHLAALWSEILEVPLELIGRHSHFFRLGGHSLLATVLLARLRQQHGQA
                     PALRQLFEQPTLKAFAALLGSSAAAPVVELRPQPRPERLPLSFAQQRLWFLEQLNPEA
                     AEYNITSAVELNGAVQLDWLQQGLASIVARHEVLRGRLVEQGYGAELLIDAESRADFT
                     IERIVAADWDGHIARCIDAETQTPFDLAAGPLIRGRLLQSEQRTLLLLTLHHTVADDW
                     SMQCLIDELAEFYRARSEGREAALPELPVQYVDYALWQRLPAQAERYREQLEYWRETL
                     GDGDYLLELPTDHPRRAEPNTLSGFHSLVLPDSLTQQLREAAQGQGVTLYVLVLAVTQ
                     VLLGRLAGQRDVRIGTAVANRDLAETQALIGCFVNTLVIRAEVDPQQAFKTFLNQVGE
                     RVLKAQEHQEVPFEQLVDALVSERDMTRSPLFQVLFAMQNARLRDDAWAPLTLREIEV
                     PSSASSFDLSWEVHDDGQRLSLRLKYKRQLFEAETITRWADAWQRLLGDAVQRPQAAL
                     GELDLLGEAERSRQLHDWNATDKTYPGPHSLAQALADQAARTPDAPALIYADQRLSYA
                     ELHTRTGAVARQLRERGVGREQVVAVCMQRSVEMVVALLGIIRAGAAYLPLDPELPVA
                     RLALMLDDAKPSLTLAQQTTVARLPQATPVWCLDALTLEDGPQPEALNQPGDLAYVIY
                     TSGSTGQPKGVLNEHGALMNRLYWMQDAYPIGADDRVLQKTPYSFDVSVWEFFWPLIT
                     GACLVVAKPDGHRDSRYLTELIQRERITTLHFVPSMLQAFVGEPELERCTSLRQVFAS
                     GEALPLDLQQRFYTRHTAQLINLYGPTEAAIDVSCWPCDPASALGFVPIGKPIANLKL
                     YVLDEALKPLPIGAVGELYIGGKGLARGYLNRAELTAERFVPSPFEIGERLYRTGDRC
                     RFLADGNIQYLGRLDHQVKLRGLRIELGEIDAVLLNQDGIEAAVTLLRDDLATPQLVA
                     YLSGPAPVDVAALKQRLGLHLPTHMVPEFIVQLPTLPLSSNGKIERKALPKPQIERQA
                     QQYQAPTSATEEVLCRLLGELLGVSQPGVNDNFFALGGDSILGLQFIAQARAHGIGLT
                     PRQLFQQRTLADLAQVASQVESCQAEQGLLEGAAPLLPIQQWLLVQQLKEPGHWSQSV
                     LLTPTRALDAEALRATLQALVEQHDALRLRFTQEHGQWRQSYADAQQALGFARVALNV
                     EYTPQALRACIAAQIEACFDLAQGPLFRALLIYLADGSQRLYLVAHHLVVDAVSWRVL
                     LEDFTTLYPRLAEGQPATLSAKTSSYRQWGQRLWQQAGSPAVQAQRDYWRQQTLPGAL
                     PADQPQADNSVADEVSLSEALSVEETQALQQAQNSYRTQGHELLIAALAGALHEWSGA
                     TAIGIDLEGHGRDAPFAELDVSRTVGWFTQLYPVRLNLPQASDTAALIVAVKEQLRAA
                     ADQAMGYGLLRHTAELPLPAHRHPLLFNYLGQVDSASGSLLQLADEPLPAHVAADTTR
                     SHELEVVLAVLGGRLQVEMKFAGLRYAPATRERLLQAYLTHLRRVIQHCQGNQAGQLS
                     PTDFPLAKALNQKSLDTLLSKLKSKPISQS"
BASE COUNT         3697 a         6885 c         7853 g         4019 t
ORIGIN      
        1 gcgctggatg ctcacggttc gccaggtcgg taccgaccag gaagtcgtct tcaccggtgg
       61 cccgttgcag caggcagttg aaggcgttga gcagcaccat aaacagcgtt gcacgctgct
      121 gctgggcata gctcttaagc cgttcggtct gggcgagggt cagggtctgg cggatggccg
      181 cgccttgata gttggggcta gccggtcgcg gcctgtccag cggcaggcgc aacagcatgg
      241 gcgcgccttg cagttggccg cgccagtaat cgatatcgcg ggccaggcgc tgctgttgcg
      301 gtgcgctgcg ctgccagtgg gcatagtcgg catactgaat tggcagcgct gctagattcg
      361 gtgtttcgcc aaggctgtag gcccggtagg cggtgatcgc ttcctccacc agaatgccca
      421 tggaccaggc gtcggtagcg atatggtgca gggtgaactg cagcacgtgc tgcctatcgc
      481 tcaggcgcag caggcgcgcg cgcagcatcg gtgggcgttg caggtcgaag gggcgtgcgg
      541 cttcctcgtc gatgtattgc tgcaattgcg tgtcgtcgtt cgcgtcgagg tgctgcaacg
      601 ccagctcaag ctccacgtcg tctgtgatga cctggcgtgg cgtgtcgcct tccaggctaa
      661 agccggtacg cagcgattcg tggcgctcga ccacatgctg cagggcggca cgcagtgcct
      721 ggacgtcgag atcaccgctc agacgcaccg cggttgagag gttgtaggcg gcgctggcac
      781 cttccagcct atcgaggaac cacaggcgtt gctgggcgta ggacaacggc aaaggttgtg
      841 cgcgcgaggc ccgcggaatc tcctcactcg cctgcttagt gggtacctga gcgtccacca
      901 cttgtgccag ttccgccacc gtgcggtgct cgaacagggc gcgcagcggc aggtcgacgt
      961 tcaaccgtcg acggatgcgc gagatcacct gggtagccag cagcgaatga ccgccgagag
     1021 cgaagaagtc gtcgtgcacg ctcagcgagg tcagcccgag cagcgtctgc cagagttcgg
     1081 ccagcgcttg ttcggtcggt gtgcgcgggg ccaccagcgc ctgcggcgca tcctcgcttg
     1141 gcaaacccag ggcggtcagc gcgcggcggt cgaccttgcc gttggcattg agcggcagtt
     1201 gttccaaccg ttgccagtgg ccgggaacca tgtaggccgc cagttgcggg cgcagatgct
     1261 cgatcagcga cgcccgggcg gtggcgtcgt ccaccgtcgc gccgtgctcg tgggcgagca
     1321 cgtagcaggc gagcagttgc aagtcgccac gggcatcggg cagggccaga actgcagcgc
     1381 tgtcgactag cggatgacgc attaggcggt tctcgatctc gcccagttcg atgcgatggc
     1441 cgcggatttt cacctgttga tcgtggcggc ccaggtactc gatcacgccg tctgcacgat
     1501 aacgtcccac gtcgcccgtg cggtagaagc gcgcgccggg ttggaacggg tggtcgacga
     1561 aggctgcgcg ggtgcgttcg gcatcgttca ggtagccacg gccgaccccc acgccgccaa
     1621 cgcacagctc gcccggtacg ccgatgggca ccgggtgcat ggctgtgtcg aggatgaaca
     1681 gctggttgtt ggcggtgggc ctgcctatcg gcatatgtgc gcagtcgtcc tgcggcgctt
     1741 cgtggatggg gtggaaggcg acgtcgtcgg aacactcggc cgggccgtag gcgttcatca
     1801 gcggtgtggc ggggaagcgc tgccgccagt cgcgcgcgag cgcgggcggc agggcctcgc
     1861 cggtgggtag cagccagcgc aggctggtga gttggtgctg cggccgggcc tcctgaagaa
     1921 ggccgcggat cagtgccggc accgcttcga gcagggtcag acgctgctgt tcgatggcat
     1981 cgagcagggc cgcggggtcg tgcgccacgg catcgggcag gatgtgcacg gtggcgccta
     2041 gcagcggcgc ggcgaggaac tgccagactg agatgtcgaa tgccggtgag gcagtctggg
     2101 cgatgcgatc ctcggcattc aggtcaagcg tcggcagctt gccgaagatg ttgttgagca
     2161 tgccgcgctg ttccaccagc gcgcccttcg gcgtgccggt ggacccggag gtgaagatca
     2221 cataggcgag ctgatccgct tggtgtggat aggtgactgc ggcaccctca ccgtgccaaa
     2281 gctgctggac caccaggcag gtgggttgtc gcgtcacccc tggcaatacc gcgtcgagca
     2341 gtccgctggc cttttcgtcc agcagcagca ggggcgcacc gctcagttcg agcagttcag
     2401 ccaggcgctg cggcggatgc tggatgtcca atgcctggaa cgcggcgccg gctttgagcg
     2461 tggcgatcat catggtcagc agcgccagac cgcgctcggc ggccagggcc accaaggtgt
     2521 ccggtccggc acccgcttgc tgcaaggcgt gagcaatggc gttggcgcgg cggtcgagct
     2581 cggcataact gagcgattcg ccggcgcaga ccgcagcgag gcgctgcggg tgtgcctgca
     2641 cctgttcggc gaacagcgca gcgtagccgc gatccagcgg gaagtcagcg gcgctctggt
     2701 tccagtccac cagcagttgt tggcgctcgg ctgcgggcag catctgcagg gtgccgaggg
     2761 ccgcatcggg ccgctccagc atctcgccga gtagttgcac caagtggccg agcatgcgct
     2821 gcaccgtgtc ggcggcgaaa cggccctggt cataggacag gcggatgccg aggcgatctc
     2881 cggggtagag caccaccgtc atcgggaagt tggtgtgtac gcggtcttcg tagatgtcga
     2941 tgctgaagct gtccagcttg acgctactga tgtccagggg ggcattctcg aacaccacca
     3001 ggctgtcgaa caattgacgg ccgcggggta tctcgctcca acgctggatg tccaccagcg
     3061 gtgcgaactc gtagtcacgc agctcggcgt tgtgcgagag cagcgcctgc agccagccaa
     3121 tcaccggttg ctcgggatcg acatcgatgc gcagtggcag gctgttgata aacagcccga
     3181 ccatctcctc aaccccagcc agcgaggtgg gacgcccggc aacggtgacg ccgaagagca
     3241 catcgcggtt gccgctgtag cgcgacagca gcagcgccca ggccccctgg atccaggtgt
     3301 tgggggtcag ttggtgctgc tggcagagtt gctgcaggcg ctgagtgcgg gtgacgctga
     3361 gggtcagatt gcagtcgccg accgcttcag gcgcacggtc cggctgggcg acggggatgt
     3421 ccacctgcag cggcgttggc tcctcgaagc cggccagttc attgctccag aaattttgcg
     3481 cgctggccat gtcctgctgg gtgagccaac tcatatagcc acggaacgaa cgaaagtgtg
     3541 ggcgcgccac cggttcctgg cgtgccacgg cctggtagat cgccagcagg tcttccatca
     3601 acaggccgaa gcaccaggcg tcggtgagga tgtgatggaa gctgcgcacc atgctgaaac
     3661 gctgctccgc cagctggaat acccgcaggt gggtgagcgg cgcacggctc atgtcgaagc
     3721 cgcgctggcg ctcgtcggcc agcatcctgt ccatcgcttc gcgctgttgc tcggcgtcca
     3781 ggtggcgcca gtcgtaccaa tcgaatgcca gctcggtgtc gcggtacagg cactgcagtg
     3841 gcgcgtggtc gtcctgccag gagaaggcgg tacgcagcat cggatgacgc tcgaggaaca
     3901 gccgccaggc ctgttccatg gccgggcgtt gcagctcacc gtcccagctg taacgctgct
     3961 gcatcaggta gataccgctg tgcggctgca acagggtgtg caacagcatg ccttgctgca
     4021 tcggtgaaag tgggtagagg tcttgcaagc gatcccaggg caatggctcg ctggccagtg
     4081 tcgcggcctg ctcgtggcac agcgggaagg cctcgctggc cgggcgcagc gccgggctat
     4141 cggccaattc ggccaaggta tgcagatggg cgatgagggc tggcgtccaa ctggccgcca
     4201 gcgttcctgt gccgtgcagc tgcaattggc cgttatgcca gcaggcatcc agggtcagcg
     4261 cactggcgcc ggccgcttca cgactggcgg cgcggatctc gtcgagcacc gcgtgtgcct
     4321 cgcggtgggc gtcccagtcg ccgagccagc gcagggtgac tggcgctgcc ggcaacgcgc
     4381 gcagcggttc cagcaggtag gggttgtccg cgagatagcg cagggtgccg tagtccgcgc
     4441 catgctgcgg gtaggcgcgc agttgtgcgg caaccgcctg caaccgttca agagcattgc
     4501 cacctggggc ctgtagatag aacggcacag gcagtgccag cggcccgagc aggcgcgccg
     4561 ggtcgagatc cgccgcggca atcggcgcgc tcagcggcag gcgctcgacc tcaggcctgc
     4621 cggccagtaa cgtcagcgcg agtggcgcgt gctcgacggt gcgcagctgt tcgaccaagg
     4681 cggccgtcag cagggtgttc cagtccagct gcagcgtctc gataagccgc ttcaggcgcg
     4741 ccgagttggc agcgtccagt cgctgctcaa cagtctggct ggaggcggac aattccaggg
     4801 ggggcagatc aaggccggca tattgcaacc agtgctccca cacctcgtcc aggcgatcac
     4861 tgctggcatc gcgctgctgc tgttcgctcc aagcgctcag ctcgccaccc ttgtacgaca
     4921 ggcggagcgg tcgctggtgg cgcagttgcg acagcgccag gttgagatcg gcgagcagca
     4981 gcggccaggc ggcctcgtcc agacacagtg gatgggcgac cagcagcaat tgatcaccct
     5041 cggcgcagcg cagcaaggtg gcgtgcaagc tttggcccac ggacagatcc aggcgcgcaa
     5101 aggcgttatc ggccagggtc tgcagcgcgg cgggcgatac gtccacttgg ggctgctcgt
     5161 ccaccgcaca aggcacgggc gcggccagca cctgctgctg ccaatcacca tcgggcgtct
     5221 gccgcagggc cagacgtagc gcctggtggt ggctttgcag cgctgccagg gcctgggcca
     5281 gttgggcact cggcacctgg ccgttgaggg cgatgcaacg ccagctggcg tttgcttcgc
     5341 cggcttccag gcgcgctcgc tggccggcgg tcagggcgat tgcaaagcct tcgctttgct
     5401 gccgggccac cgctgtttcg gcaccggagc cgagcacctg ggcgatatcg gcgatggtac
     5461 ggttgtcgaa cagctgcttg ggcgtcaact tcaacccttg ctggttggcg cgggcgatga
     5521 tctgcaggtt gaggatcgag tcgccaccaa gggcgaagaa attgtcgtgc acgccaaccc
     5581 gctcgacttt cagcacgtcc tgccagatac gcgcgagcag cgtttccacc tcattgcgcg
     5641 gggcaacgtg gctggcaccg tcatcgaccg gcgcgctggg cagcggtaac tggcgcagat
     5701 ccggcttgcc gttggcggtc agcgggatgg cgtcaagggt gatgacgtgc gccggcacca
     5761 tatagtccgg tacgcgccgg ccgaggtcct cgcacagctt gttggcggac agcgattggc
     5821 gggccaccac gtaagcgacc aagcgttgcg tgccatccac atccagcacg cgtaccagta
     5881 cctgatcgat cagcggcgac aggcgtttga tctgcgcctc tacttcgccc agctcgatgc
     5941 gattgccgcg aatttttacc tggttatcga cccggccgag gaaggccaac tggccgtcgt
     6001 tcagccagcg cacgcggtcg ccggtgcggt accagcgctg ctggtcggca tcgacgaaac
     6061 gttcggcgct cagctgcggg tggtgcaggt agccgcgcgc caggtcgcca gcgatccaca
     6121 gctcgccggg tacgccagtg gccgcgctgc gcccgctgtt atcgacgacg cgcaattggc
     6181 gattggccag cggccggccc agcgggacca ggccaacagt cgcctgtagc gcagtggcca
     6241 ccgcaccgac cgtcgcttcg ctggggccgt agtgattgaa cagctgtagt gtcggggcca
     6301 gtgcccgcac ctgctcaagc agcgcaggtt tgagtgcatc gccgccgaat accaggcggg
     6361 cgcggggcag caggcgtggg tcggggtgcg cctggagcag gccgtgtaga tgcccggggg
     6421 tgattttcag cacgtccacc gggtgctcgc cgaagaaatc ggccagggcc agcggatcga
     6481 agctggtgtc ctcatccacc agcagcaggc tgccgccgct gcatagcgcg ccgaacagct
     6541 gggtatggcc gaggtcggcc gccagtgtcg acagcagcgc atagcgcgcc gcaggcggcg
     6601 ggtcgagcag ttgggccacg gcagcgaggt agccgacgat ggcgcggtgt tcgaccagta
     6661 cgcccttggg tatgccggtg gagccggagg tgtagatgac ataggccggc gcggctgggt
     6721 catgggccag cggtgcggcg ctggatggca gagcggcgat ggcggcggct tggtcgtcca
     6781 gccagagcct cgccggtgct tcgtcacgcg gcagacgtga ctccagcggg cggcggctga
     6841 tcagcagatc ggcgcggctg tcggcgagga tgtccgccag gcggcctgcg ggctgtttga
     6901 gatcgagcgg caggtaggcg gcgccgctct tgagggtggc caacagcgcg atgacgaagt
     6961 cgacgctgcg ctccagcgcc agggcaacca gacggcccgc gccaatgccc tgcgcttgca
     7021 ggtgatgggc caggcgattg gcctcggcgt ccagctcgcg gtagttcagg gcgcgctggc
     7081 agtcgcgcac tgccaactgc tcggggcttg ttgcggccca atgggcgaag cgctggtgca
     7141 gcgtctcggc cgcgacctcc aggtgtgcgc cctggccata ggcctggacg cgctggcgtt
     7201 cgtcgtcgct cagccagtcc agcgcctgca cacgggcctg cggttgttcg gccatggcca
     7261 gcaggatcgc gcgtaactgg gcggacaggg tggcgatctc gccgtcgctg aaacggctgc
     7321 gctcataact gaacaggtac tccaggcgct cgcccggcac cacgatcaac gacagcggaa
     7381 agtggttgcg tccgtgggtc agcttgatgc catcgtcgcg ccgcagttct ggcggcgcgc
     7441 tgaagcgcag gccgtcggcg tcctgattga gcgcgtcggt gaccggaaag ttttcgaaca
     7501 ccaggatcga gtcgaacagc gcccgttcgg cgggcacctg gcttagcggc ttgagtcggc
     7561 tcagtggcag gtaggcgtgc tggcgcaggt cgctattgcg ctgctgcagg gcctgcagcc
     7621 agtcgctcag ggggcgctcg tcggtcagtt gcacgcgcag cggcagggtg ttgataaaca
     7681 gaccaaccat gctgtccacc gacgccagtg catccgggcg gccagcgacg gtaacgccgt
     7741 agaccacttc gtcgcgtcca gcgtgctgcc cgagcagcaa ggcccaggca ccctggatca
     7801 gggtgttgac ggttaggtga tgctggcgcg cgaactgcgt caggtgtgcg gtttcagcgg
     7861 cgtcgaggcg cgtctcgcgt tccgcatagg gatgctcctg cggcgcctcg ggcggcgggg
     7921 cgaaacttgg tagcggcgtg ggctcgctga cctcttgcag ctgctcgcgc cagaaacctt
     7981 cggtggcgtc tgcgtcctgc tcgcccagcc aggcgatgta gtcgcggtat ccgcggctcg
     8041 gcggcagact gaatgcacgg ccgcgcagtt gctcgtagta cagggccagc cattcctgaa
     8101 tggccacgcc cacgctccag ccatccataa ggatgtggtg aaaggtccag atcaggtgcc
     8161 agcgttgttc gggcaggctt accaggcaca ggcgcatcag cggtgcctcg ttcagctcca
     8221 gcgcttggtc gcgctccagc cgtgcacgtt ggcgcagggc gtcctcggct tcttcgttat
     8281 ggtcgcgcca atccagttcg ctcaggggca ggggtacttc gtgctggacc acctggtagg
     8341 catcgtccag gtcttcccag agaaaggcgg tgcgcagtac tgcatggcgc tgcatcagcg
     8401 cctgccaggc acggcggaag gcaccggtgt ccagcgggcc ggtcatgtcc aggctgagct
     8461 gctggtagta cacgcgcgaa tccggctcat agacgctgtg gaacagcagc ccgtgctggg
     8521 tgggcgagag ggagtagatg tcctcgatgt tgctcatgat tctgcttcca gtccgtaggc
     8581 cgtaaaaacg tatcgagctt cgcgtcggca cccggcgact ccgaggagtc gaccggcgtg
     8641 ccagccgcta gctttgcgag ataggtttgc tcttgagttt gcttaacagg gtgtccaggc
     8701 tcttctggtt cagggccttg gccagcggga agtccgtcgg cgacaactga cccgcctggt
     8761 tgccctggca gtgctggatg accctgcgca gatgcgtgag gtaggcctgc agcagacgct
     8821 cgcgggtggc cggggcgtag cgcaggccgg cgaacttcat ctccacctgc aagcgaccgc
     8881 cgagcaccgc gagcaccact tccagctcgt ggctgcgggt tgtgtcggcg gcgacatgcg
     8941 ccggcaacgg ctcgtcagcg agctgcagca ggctgccgga ggcgctgtcg acctggccca
     9001 gatagttgaa tagcagcgga tggcggtgcg ccggcagcgg cagctcggcc gtatggcgca
     9061 gcaggccata gcccatggcc tggtcggccg cggcgcgcag ctgttccttg accgcgacta
     9121 tcagcgcagc ggtatcgctg gcctgcggca ggttcaggcg caccgggtag agctgggtga
     9181 accagcccac cgtgcggctg acgtccagct cggcgaaggg cgcatcgcgc ccgtggcctt
     9241 ccagatcgat gccgatggcg gtcgcgcccg accattcgtg cagcgcgccg gccagggcgg
     9301 ctatcaacag ttcgtggcct tgggtgcggt agctgttctg cgcctgctgt agggcctggg
     9361 tttcctcgac gctcagcgcc tcgctcaggc tcacttcatc tgccactgag ttgtccgcct
     9421 gcggctgatc cgccggcagt gcgccgggca gcgtctgctg ccgccagtag tcgcgttgcg
     9481 cttgcacggc ggggctgccg gcctgctgcc acaggcgctg gccccactga cggtaggagc
     9541 tggtctttgc tgacagcgtg gcgggctggc cctcggccaa gcgtgggtag agcgtggtga
     9601 agtcttccag cagcacgcgc caggacaccg cgtccaccac caggtggtgg gccaccaggt
     9661 agaggcgctg gctgccgtcc gccaggtaga tcagcagagc gcggaacagc gggccttgcg
     9721 ccagatcgaa gcacgcttcg atctgcgcgg cgatgcaagc gcgtagcgcc tgcggcgtgt
     9781 actcaacgtt cagggcgacc cgggcgaagc ccagggcctg ctgcgcgtca gcgtagcttt
     9841 gccgccattg gccgtgctcc tgggtgaagc gcaggcgcaa ggcgtcgtgc tgctcgacca
     9901 gcgcctgcag cgttgcgcgt agcgcctcgg catccagtgc tcgggttggc gtcagcagta
     9961 ccgactggct ccagtgacca ggctccttca gctgctgtac cagcagccac tgctggatcg
    10021 gcagcagtgg cgccgcgcct tccagcaatc cctgttcggc ttgacagctt tcgacttggc
    10081 tggcgacctg cgccaggtcg gccagggtgc gctgctggaa caactgccgc ggtgtcaggc
    10141 cgatgccatg ggcgcgggct tgagcgatga actgcagacc gaggatggag tcgccgccca
    10201 gggcgaagaa gttgtcattg acccctggct ggctgacgcc gagcagctca ccgagcagcc
    10261 ggcatagcac ttcctcggta gcactggtcg gtgcctggta ctgctgcgct tggcgttcaa
    10321 tctgtggttt gggcaacgcc ttgcgttcaa tcttgccgtt gctgctcaac ggcagggtag
    10381 gcagttgcac gatgaactcc ggcaccatat gcgtcggcaa atgcagccct agccgctgct
    10441 tgagggcggc tacgtccacc ggcgcaggac cgctgagata ggccaccagt tgcggtgtag
    10501 ctagatcgtc gcgcagcagg gtcaccgccg cctcgatgcc atcctggttc agtagcacgg
    10561 cgtcgatctc gcccaactcg atgcgcaggc cgcgtagctt gacctgatgg tcgagacggc
    10621 ccaggtactg gatattgccg tcggctagga agcggcagcg gtcgccggtg cggtacaggc
    10681 gctcgcctat ttcaaacggg ctcggaacga aacgctcggc ggtcagctcc gctcggttca
    10741 ggtagccgcg tgccaggccc ttgccgccga tatagagctc gcccaccgcg cctatgggca
    10801 atggtttgag tgcttcatca aggacgtaca gcttgagatt ggcgatgggc ttgccgatgg
    10861 gcacaaagcc cagggccgaa gctggatcgc agggccagca actgacgtcg atggccgctt
    10921 cggtggggcc gtagagattg atcagctgcg ccgtgtggcg cgtgtagaag cgctgttgca
    10981 gatcaagcgg cagtgcttcg ccactggcga agacctggcg cagtgaggta cagcgctcca
    11041 gctctggctc gccgacgaag gcctgcagca tcgagggcac gaagtgcagg gtggtgatgc
    11101 gctcgcgctg gatcagctcg gtcaggtaac ggctgtcgcg atggccatcc ggcttggcaa
    11161 ccaccaggca ggcaccggtg atcagcggcc agaagaactc ccacaccgac acgtcgaagc
    11221 tgtatggcgt tttctgcagg acgcggtcat cggccccgat ggggtaagca tcctgcatcc
    11281 agtacaggcg gttcatcagc gcgccgtgct cgttgagcac gcccttgggc tgaccggtgg
    11341 agccggaggt gtagatcacg taagcgaggt cgccgggttg gttaagcgcc tcgggctgtg
    11401 ggccgtcctc cagggtcaag gcgtccaggc accagaccgg cgtggcctgc ggtaagcgcg
    11461 cgaccgtggt ctgttgcgcc agggtcaggc ttggcttggc gtcgtcgagc atcagcgcca
    11521 ggcgggcgac cggtagttcc gggtcgagcg gcaggtaggc ggcaccagcg cggatgatgc
    11581 cgagcagggc tacgaccatc tctaccgagc gctgcatgca tacggcgacc acctgttcac
    11641 ggcccacgcc gcgttcgcgc agttgcctgg ctacggcgcc tgtgcgggtg tgcagttcgg
    11701 catagctaag gcgctggtcg gcgtagatca gcgccggcgc gtccggtgtg cgcgcggcct
    11761 ggtcggccag tgcctgggcc aggctgtgcg gaccggggta ggtcttgtcc gtagcgttcc
    11821 agtcgtgcaa ttggcggctg cgctcggctt cgccgagcag gtcgagttcg cccagcgcgg
    11881 cctgcgggcg ctgcactgca tcgcctagca gacgctgcca ggcgtcggcc cagcgtgtga
    11941 tggtctcggc ctcgaacagc tggcgcttgt acttcaggcg cagcgacagg cgttggccgt
    12001 cgtcgtgcac ctcccagctc aggtcgaagc tcgatgcgct ggacggcacc tcgatttcgc
    12061 gcagcgtcag cggtgcccag gcatcgtcac gcaggcgcgc gttctgcatg gcgaacagca
    12121 cctggaacag cggcgagcgg gtcatgtcac gctcgctaac cagtgcatcg accagctgtt
    12181 cgaagggcac ttcctggtgt tcctgggcct tcagcacgcg ttcgccgacc tggttcagga
    12241 aggttttgaa cgcctgctgc ggatcgacct cggcacgaat caccagggta ttgacgaagc
    12301 aaccgatcag cgcctgggtt tccgccagat cgcggttagc caccgcagtg ccgatacgca
    12361 catcgcgctg gccggctagg cgaccgagca gcacctgggt gacggccagc accagcacgt
    12421 agagcgttac gccctggcct tgcgccgcct cgcgcagttg ttgcgtgagg ctgtccggca
    12481 gcaccaggct atggaagccg ctgagggtgt tcggttccgc ccggcgcgga tgatcggtgg
    12541 ggagctcgag caggtagtcg ccatcgccga gggtctcgcg ccagtattcc agctgctccc
    12601 gataacgctc ggcctgggcc ggcaagcgtt gccatagggc atagtcgaca tactgcaccg
    12661 gcaattcggg cagtgccgct tcacggcctt cgctgcgggc acggtagaac tcggccaatt
    12721 cgtcgatcag gcactgcatt gaccagtcat ctgcgacggt gtggtgcagc gtcagcagca
    12781 acagcgtgcg ctgctcgctt tgcagcaggc gaccgcggat cagcgggccg gcagccaggt
    12841 cgaagggtgt ctgggtttct gcgtcgatgc agcgcgcaat gtgcccatcc cagtctgctg
    12901 caacgatccg ctcgatggtg aagtctgcac ggctttcggc gtcgatcagc agttcggcac
    12961 catagccctg ttctactagg cggccgcgca gcacttcatg tcgcgcgact atgctggcca
    13021 gaccttgctg cagccagtcg agctgtactg caccgttcag ttcgacggcc gaagtgatgt
    13081 tgtattccgc ggcttccggg ttgagctgtt cgaggaacca caggcgttgc tgggcgaagg
    13141 acagcggcag gcgctcggga cgcggttgcg gccgcagttc aacgacaggc gcggcagcgg
    13201 agctaccgag cagcgccgcg aacgctttca acgtcggctg ttcgaacagc tggcgcaggg
    13261 cgggtgcctg gccatgctgt tgacgcaggc gtgccagcag cacggtggcc agcaatgaat
    13321 gaccacccag gcggaagaag tggctatgac ggccgatcag ctcgagcggc acctcgagaa
    13381 tctcactcca gagtgcggcc agatgctgtt cggcctcgcc ttgtggggcc tcaaaggtat
    13441 cgccggatag ttccagatgc ggctttggca gttgtttgcg atccagcttg ccattgctat
    13501 tgagcggcag agcggccagc tctaccacca cgcttggcac catgtgcgca ggcagcgcct
    13561 gcgtcagggc ggtttgcagt tgctctacgt ccagcccgct tgcggatgca accacatagg
    13621 ccaccagttg cggcgtgccc agatcgtcgc gcagcagggt ggcggcctca cgtacgcagg
    13681 gttgagcgag cagagcggcg tcaatctcgc ccagttcgat acgttggcca cgcagcttga
    13741 cctgatggtc gaggcggccc aggtactgga tgttgccgtc ggccaggaag cggcagcggt
    13801 cgcccgtgcg atacaggcgc tcgccagcct tgaacgggct ggcgataaag gccgtggcgg
    13861 tcagctcggg gcgattcagg tagccacgcg ccaagcccgc tccgccgatg tagagctcgc
    13921 caaccgcgcc gagtggcagc ggattaaggc cctcgtcgag gatatacagc tggatgttgg
    13981 cgatgggttt gccgatgggc acgaagccca gcgtcgaggc gtcgtcgcag atccagacgc
    14041 tgacgtcgat ggcggcttcg gtgggcccat aaaggttgat caggcgggcc gggtggcgcg
    14101 tgcagaagcg ctgctgcaag gccagcggca gcgcctcgcc gctggcgaag acctggcgca
    14161 gactggtgca gtgctcaaga tctggttcgc taacgaaggc ctgcagcatg gacggcacga
    14221 agtgcagggt gctgatgcgc tcgcgctgga tcagctcccc caggtagccg ctgtcctgat
    14281 gtccgcctgg tttggcgagc accaagcaag caccagtgat cagcggccag aagaactccc
    14341 acaccgacac gtcgaaaccg tagggggttt tctgcagtac gcggtccgtc gcatcaagcg
    14401 ggaaggcctc ctgcatccag tgcaggcggt tcatcaacgc gccgtgctgg ttgagcacac
    14461 ctttcggctg accggtggag ccggaggtgt agatcacgta ggcgagatcg cccggctgat
    14521 tagcgactat acggggcgtg ccgacgatct cgggcaacgg ctgatccagg caccaggccg
    14581 gcacctgcgc tgccaaccgg tcacgccact ggctctgcgt cagcaccagc gggcaggcgg
    14641 catcgtccag cataaaggcc aggcgggcag ggggcaggtg cgggtccagc ggcaggtagg
    14701 ctgcgcccgc cttgatgatg gccagtagtg ccacgagcag atcgctgcaa cgttccaggc
    14761 ataccgcaac cacctgttcg cggccaatgc cctgggcggc cagggcctgc gccagatggt
    14821 ctgcgcgctg gtgcagcgct tggtaggtca gggtttcgcc ttcgcagacc agtgcaggtg
    14881 cgtcggggct gcgcagcgcc tgttgcgcca gggcttcgct gagagttgcg aggccgggat
    14941 agtgacgggc ggtatcgttc cacgtcagca gctggcgctg gcggtcggcc tcgctcagtg
    15001 gcgagagctg ggcgagcggg gtgttcacgg catccggcag ttggccgagg aagtcctgcc
    15061 actggactag ccaggcctgc atggttgcct catggaacag gcaggcgcgg tactccagca
    15121 tcaccgacaa ctgctggccg tcgtcgtgga cttcccagtt ctgttcgaac ttggccgctg
    15181 acgagcggat atccagtggc tgcaggctca gatccggcca ctgcaggggc gcgcgctcgc
    15241 tgttctgcat gacgaaagcc acctgaaaca gcggcgtgcg cgacagatca cgctccggcg
    15301 ccaagtgctc gaccacctgc tcgaagggca catcctggtg ctcctgcgcc atcagcacgg
    15361 tttgccgcag ctgggccagg tagtcgccga aggtctgctg tggggtcagt tcggagcgga
    15421 tcaccagggt gttgacgaag caaccaatca agggctgcac gacgtcgagg cggcggttgg
    15481 cgaccggggt gccaatacgc atgtcctgct gcccgctgta gcgactgagg aacagcttga
    15541 gaccggccag taacagcatg aagggcgtga cattctgctc gtgggcgaac tgccgcagcc
    15601 gcgtggtcag cgcctgcggc agacgggcga agcaggtgcc ggcagtgttg tcgaacgtcg
    15661 ccggacgcgg caaatcggtt ggcagttcga gccggtagtc gccgttctcc agggtcgtct
    15721 gccagtagtc caactgctga cggtagatct gctggcgggc cgcactgcgt tgccacacgg
    15781 cgtagtccac gtactgcacc ggcagcgtcg gcaagtcggg tcggcgcttt tccagaccgg
    15841 cgcggtagcc ttcggcgagt tcctccagca gcacctgcag tgaccagccg tcggcggccg
    15901 agtggtgcag gccgagcaac agcagggcct gaccgctggc tgggtggcgg atcaggtgca
    15961 cgcgcaacgg atgttcgcgc gccaagtcga acggctggcg cgcctcggcc tcggcgacct
    16021 gacgttggtg ctccggccag gccgccgcgc tgccctggta atcctcgaca accagattga
    16081 agaaggccgg ggcgtcgatc agcagttcag cgccgtcgcc tttgtcgagc aggcggctgc
    16141 gcagaatggt gtgacgttcg acgagcgttt cgaaggcctg acgcagtatt tccagacgga
    16201 gttcgccggt cagagtcagt gccgtgatga tgctgtattc gccggtgtcc ggtgccaatt
    16261 gctcaaggaa ccacaggcgc tgctgctcga aggacagcgg cagacgtgcc gggtgcggcc
    16321 cagctaccag cgtcggtaag gtgtggttcg gctgcgctgt ggtgccgtcg atgctgcgtg
    16381 cctgtgccgc cagcgtcggc tgttcgaaca ggctgcgcag agctggagcc gtgtcgaata
    16441 gcgcagcgat gcgggtcagc aacacggtcg ccagcagcga gtggccaccg aggtggaaaa
    16501 agttggattg gcgaccgatc tgctccagcg gcacctgcag cgtttccgcc cagaggcttg
    16561 ccaagcggac ttcgctgtcg ccctgtggcg cttccaaggt ttcccctgct tcctccagta
    16621 gcggttccgg caacgcgcgg cggtcgatct tgccgttggc gctcagcggc cattcgctca
    16681 aggctaccag cgcctgcggc agcatatagg cgggaataaa acgtcccagc tcatcgcgcc
    16741 acccttcgct cggtagtttg cctgtggccg tcagtacata ggcgataagc ctgtcgtcgc
    16801 gcaccagtgc cagactgtcg cgcacaccgt ccaggcgccg cagtgcctgc tcgatctcgc
    16861 ccggctcgat gcgcaggcca cgcagcttga tctggaagtc tttacgtccg agaaattcga
    16921 gttcgccgtt agccagatag cggcccaggt cgcccgtgcg gtacagccgg ccctcgccga
    16981 aggggttggt gacgaatacg ctggccgtca atgcctcgcg atgcaggtag ccgctaccca
    17041 ccccggcccc ggccacgcag atttcgccga tcgcgccgac cggtagcggc tggtcgtagc
    17101 gatcaagtac gtagagctgg ttgttatcca ccggccggcc gatgggcagc acctcgtttg
    17161 gcgcgtcgat atccacttcg tgccaggcca ccacgtcggt gcattcggtg ggcccgtagc
    17221 tgttgatcac gcggcagcgc ccgtgctcgc tggcgagcca gccgtgcaga cgctccatgc
    17281 ggatcggttc gccgcccagt accagcgcgc gcaggctggc caggtcgcgc cactgctgcg
    17341 cgtcggcgat ttccaccagc gggtagaaag cgctgggcgc accgttgagc agggtgatgc
    17401 ggtggctgcg gatgtcgtgc agcagtgcct gcgggtcata ggactccacg ctgggcataa
    17461 ccagggtggc accgcacagc agcggggcga agatgttctt ctgggtcagg tcgaagccgg
    17521 tggcgctagc cagcagaacc ttgtcgccct gacgccagcc caatgtgcgc aggtaccagt
    17581 gctgcaggtt gcgcacgccg ccgtgggtga ccacagcacc cttgggctgg ccggtggaac
    17641 cggaggtgta gatggcgtag aacggcgagt ctgcgtcgag gcagcgtgtc ggcggcgttg
    17701 ctggctgttg ctcgagttgc gcctgcagtc ggtccagttg cagcaacttc actggcagtt
    17761 cggtgtagcg cgccgcgtat tggccatggg tcagcagcag atcgctgcca cagtcgtcga
    17821 ggatgtagcg gatacgttcg gccggataac tggtgtcgat tggcacgtag gcggcaccgc
    17881 atttcagcac tgccaggatg gcaaccgcca gttctggaga tttatccagg cacagcgcca
    17941 cccagtggcc gcggcctacg ccctgtgcga tcaggtggtg cgccaggcga ttggcctggg
    18001 tatcgagttc gccgtaggtc agccgatgtt cgcggtgttc gatcgccaac gcatccgccg
    18061 agcgcacaac ctgggcttcg aaggcgttga ggatggtcgg tgctagcggt agggcttgcg
    18121 ccgtacggtt ccagccctcc accgtgcgtt gccattcatc cggtgttagc aggcgatgct
    18181 cgcccagcgg gcgctcaggc gcctcgagca tggcctcgaa aaggtggaca aaacgttgca
    18241 gtaaacgctc agcgctgggc gcttcgaaca ggtcatgggc gtacagcagg ttgagttcga
    18301 tggggccctg cgatggggtg acgatatgca ggtccaggtc gtattcagag tggctaagtg
    18361 gtgtgtccag cacctcgaac tcaaggccag ggaactgcac gcgacgctcc attggtaact
    18421 gctgcgcgac cattatctgg aacagcgggg cgtgactcag gcggcgcggc agtgccaggt
    18481 ggtcgaccag acgttcgaag ggcaggtcct ggtggcgttg ggcgtcgagc agggtgtcgc
    18541 tgacctgtcg cgccagctcg gcgaaactcg gctggccttg gaagcgcacg cgtaccggca
    18601 ggttgttgag cagaatgccc accaggggtt gggtttccga ccgctcgcga ttggacaccg
    18661 gggtgccgag gatcagatcg tcctgaccgc tcatgcgctg caggaaactg gcgaaggcgg
    18721 cgaacaccag gctgaacggc gattgttgca ggcgcgccgc cagtgcgcgg gcgcgctcgc
    18781 tggccattgg cgacagctgg gcggcgagca tgccgctgcg gtgcgattgg ctggccgggc
    18841 gcggcttgtc ggtcggcagc tcaagcaggt agtcctgatc tgcgccgtca ttgcgggcga
    18901 gctgatgttc ccagtaatcc agatgcggct gtagcgctgc cggttgcagg cgctgctgtt
    18961 gccaaaggct gtagtcagcg tactggattg gcagcggcgc cagtcgcggc gtctcgccgg
    19021 cctgcagggc agcgtagaac tgggtcagct cgcgcagggc cagggtcgct gaccaggcat
    19081 cggagaccag gtggtgcagg gtgatggcca ggacgtgctg gcgttcgccc aaatgcaaca
    19141 gccgcgcgcg aatcagcggc gggctgacca gatcgaacgg ttgcgcgaac cagctgctca
    19201 gctcggtttg cagcgtagcg tcactgacat ctgtcaggtg actaatcggt agttcgaaac
    19261 tcaacgcctc gacgattcgc tggtagggct ggccgtcctc gctggggaaa gcggtacgca
    19321 gtacctcatg acgttcaagc aaccactgga aactggcgcg cagcgcctca atgttcagct
    19381 cgccgcttag gcgcagggcg aagggaatat tgaaatgggc gctaccaggg tttagctgtt
    19441 cgacgaacca caaccgctgc tgggcgaaag acagcggcag gcgcggcggc cggggctgta
    19501 cggcaatggc gtcggcgtct gccgggccgt cgctggtagc ggccagttgt tggtcgatgg
    19561 cctgcgccag ttgacgcacg ctggggcact cgaacagcgc tttgagcggc agctcgatgc
    19621 gaaagactgc gcgaatcttc gataacattt gagtggccag cagcgagtgg ccgccgaggg
    19681 cgaagaagct gtcggtaacg ccgaagctct ccagcgccag gacttcctgc cagatgccgt
    19741 gtagcgcctg ttcggtctca ttggctggtg gagtcgactc gctctcgctc cattgcagct
    19801 cgcgcaacac caggcgtttg cgatcgacct tgccgttggc gttgaggggc agggcatcca
    19861 gttcgaccag gcgcagcggc accatgtagg ccggcaactg acggctcagg gcttccttca
    19921 gcgtgagcgc atcgacagcc tcggcggtgt ggccgaccca gtagccgacc agttgcgatt
    19981 cgttacgggc gtctttgtgc accaccaccg ctgccgcttt tacggccggg tgctgctgca
    20041 gcttggcttc gatctcgccc agttcgatgc gatggcctcg gatcttcacc tgctgatcgc
    20101 ggcggccgag gaattcgatc acaccatcgg cgcgatagcg ccctagatct ccggttctgt
    20161 agaaacgcgc gccaggctgg aacgggtgat cgacgaacgc cgcccgagta cgctcggcat
    20221 cgttcaggta gccgcgcccg acgccagcgc cagccacgca tatttcaccg ggcacgccca
    20281 ccggcagtgg gcgcagttcg gcgtccagca cgtagagttc ggtgttcggt gtaggcctgc
    20341 caataggcat ggcctgggct tgctcgtccg acgccgcatg gatagcgtgg aaggccacat
    20401 cgtcggcaca ttccgccggg ccgtaggcgt tcattaatgg cacatgggtg aagcgtgcga
    20461 accagtcgcg gcacagcgct ggcggcagtg cttcgccagt cggcagcaac cagcgcaagg
    20521 cactgaggtc ggtggttgcc gtgctggccg tgagtactcc gcggatgacc gcaggtactg
    20581 cttcccagag tgtcagttgc tggttttgca gtacctctag caggcgctct gggtcatggc
    20641 taacggcgtc cggcagcata tgcacggtgg cccccagcag cggcgcagcg agcagttgcc
    20701 agacggaaat atcgaaggct accgaggcgg tctgagcgat acggtcctgc tcgctgaggc
    20761 caaggctcgg caccttgcca tagatgttgt tgagcatgcc gcgctgttcc accagtgcgc
    20821 ctttcggcgt gccggtggag ccggaggtaa agatcacgta agccagatcg tccgccgtgc
    20881 cgcggggttc tatgagtggg gcggggccgc tcaaccagag tggttcaata ccaatcactc
    20941 gcggcgcttc tgccccgaac cgggtacaca gctcatcggc cagcgcgcac tggctgtcgc
    21001 cgaccagcat cagcggggct cggcttagct cgaggatttc cgccagacgc tgcgccgggt
    21061 gcttgacctc caggggcagg tagcaggcac cggctttgaa gatcgagatc agcgccgtga
    21121 ggtgggccag gccgcgttcg gcgagcaggg ccaccggttg gtttggcccg gtaccggctg
    21181 tgatcagcgc tgcagcgagg cggttggccc gttcatccag ttcggcatag ctgagttgct
    21241 cgccttcgca gacaaccgcg accttgctcg gctgacgtgc cacctgttcg gcgaacaggc
    21301 tggcataggt acgctcaagc gggaagggtt gttgggtacg gttccactcc accagcatct
    21361 gctggtgttc tgcgtcgctc agtagctcca gtgaggcgag gctggcgctt ggtgcagcga
    21421 ggatgccgag gacgatctgg cgcagatggc ccagcagacg ttcgatgctg tcgcgggtga
    21481 aacgtcgacg atcgtaggag atgcgtacgg ccaactgttc gccgggcatg accaccacgg
    21541 tgaggccgta gtgggtgtgc acgctgtgtt ccatgtcgtc caggctgaag ctcagttcgc
    21601 cgctgaacaa cttgtcgtcg atgggggcgt tttcgtagac caggatgctg tcgaacaact
    21661 cctcgccgcg cgggaaggcg ctccagccct ggatatccac cagggcgctg tgttcgaact
    21721 cgcgcaggcc gaggttgtag cgttgcagtg cttgcagcca gccgatgcgg tcctgctgtt
    21781 catcaatctg tacgcgcagc ggcagggtgt tgataaacag cccgatcatc tcttccacac
    21841 ctgccagctg tggtggacgg ccggcaacgg taacgccgaa gagcacgtcg cggtggccgc
    21901 tatagcgcga cagcaacagg gcccaagcgc cctggaacag ggtgttggga gtaatccgct
    21961 ggttctggca gagggcagtc agttcgcggg tctcgcgcgc gtcaaaacgg atcagcgtat
    22021 cgtcaaccag caccgcttcg ctgtactggc tttggcgtgt ctggcgctcg atgaccagcg
    22081 gcgttggggt gtcgagccct tgcagctgtt gctgccagaa cgcctgcgcc tcgggcaggc
    22141 tctgtttctg cagccaggct atgtagtcgc ggtacggacg aggcttgcgc atcgtcggcg
    22201 tacgcccctg gcgcagggca tcataggcgt cgatacagtc gaccatgatc agcgagatgc
    22261 accaggcgtc catcagaatg tggtggaagc tgcggatcat ctcgtgccgg cctgcaccca
    22321 gacgcaccag gcgaactcgg atcagcggtg gcttggctag gttgaagccg ttgcgctgtt
    22381 cctgctctag cagcgcctgg atgtgcgctt cctgctgcgc ctcactgagg ccctcaaggt
    22441 cgatgaactc gacg
//
LOCUS       DASRUM010000269         4604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1798702, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000269 DASRUM010000000
VERSION     DASRUM010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798702"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VFF22_18130"
     CDS             complement(<1..725)
                     /locus_tag="VFF22_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HZX18774.1"
                     /translation="MQSGVWALQPLADMTATEFRDWQTLLEERTGVVLNERRRAFLQT
                     NLSARMRELGVMDYATYYRQVTDGPRGAVEWSTLLDRLTVQETRFFRHRPSFEVLESY
                     LRERLQQGVTQPWELWSVGCASGEEPYSLAISAAEVLRESEHPDHFGVTGTDISLNAL
                     SKARDGQYGARRLEQLDADLCQRYFLAQDDGRFKVVPNLAARVCCARLNVLELAKAPM
                     SGMDVIFCQNLLIYFRRWRRREIL"
     gene            complement(794..2839)
                     /locus_tag="VFF22_18135"
     CDS             complement(794..2839)
                     /locus_tag="VFF22_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX18775.1"
                     /translation="MKKLNAGNLLAGARSSTLIAGLFIVLIVSIVLLFANFAYINTQS
                     NYDTEYIGHAGELRVLSQRIAKNSTEAAAGTAEAFALLRDARNDFQTRWGYLTDGNEE
                     TGLPAAPESLQPQIAVVQQDWDTLRQNTDAILANEQTVLSLHQVAATLAETIPQLQVE
                     YEEVVDILLESGAPAAQVSVAQRQSLLAERILGSVNKVLAGDQDSVQAADMFGRDASL
                     FGRVLSAMLEGNAAMEITQVSDEEALERLAEISELFEFVSGSVDEILETSPELFQVRE
                     SANVIFSVSQTLLDKASDLAVGFDNLATGRAINTLIGYVLGALALGSIILIGLVMVRE
                     TNRRLASTAEKNERNQAAILRLLDEIADLADGDLTVAATVTEDFTGAIADSINYSIDQ
                     LRDLVATINLTAVQVAGAAQETQATAMHLAEASEHQAQEIAGASAAINEMAVSIDQVS
                     ANASESSAVAERSVAIANKGNEVVHNTITGMDNIREQIQDTSKRIKRLGESSQEIGDI
                     VSLINDIADQTNILALNAAIQASMAGDAGRGFAVVADEVQRLAERSSAATKQIEALVK
                     TIQTDTNEAVISMEQTTSEVVRGARLAQDAGVALEEIEKVSKTLAALIQNISNAARQQ
                     ASSAGHISNTMNVIQEITSQTSSGTTATAKSIGNLAKMASEMRKSVSGFTLPGGEQA"
     gene            complement(2924..3460)
                     /locus_tag="VFF22_18140"
     CDS             complement(2924..3460)
                     /locus_tag="VFF22_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013984316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZX18776.1"
                     /translation="MSDAQTPFQTLQDIDRLCRSLAAGLPSQQAVVQTWSGIGFRMGE
                     RFFVAPMGEVGEVLPEPRHTLLPGVKSWVKGVANVRGRLLPVMDLCGFFGNELSPLRK
                     KRRVLVVDHQEVFAGLTVDEVFGMQHFPVDTFSEQLPPLEASIAPFIHGVFQREQPWL
                     VFSPHALAQNPGFLDVAY"
     gene            complement(3506..3871)
                     /gene="pilH"
                     /locus_tag="VFF22_18145"
     CDS             complement(3506..3871)
                     /gene="pilH"
                     /locus_tag="VFF22_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilH"
                     /protein_id="HZX18777.1"
                     /translation="MARILIVDDSPTEMYKLTAMLEKHGHQVLKAENGADGVALARQE
                     KPDAVLMDIVMPGLNGFQATRQLTKDADTSHIPVIIVTTKDQETDKVWGKRQGAKDYL
                     TKPVDEETLVKTLNAVLAG"
     gene            complement(3919..4326)
                     /gene="pilG"
                     /locus_tag="VFF22_18150"
     CDS             complement(3919..4326)
                     /gene="pilG"
                     /locus_tag="VFF22_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HZX18778.1"
                     /translation="MEQHSEGLRVMVIDDSKTIRRTAETLLKKVGCEVITAVDGFDAL
                     AKIADTHPNIIFVDIMMPRLDGYQTCALIKNNSAFKSTPVIMLSSKDGLFDKAKGRIV
                     GSDQYLTKPFSKEELLGAIKAHVPDFVPVEQAS"
BASE COUNT          956 a         1378 c         1264 g         1006 t
ORIGIN      
        1 aggatctcgc ggcggcgcca gcgacgaaaa tagatcaaca agttctgaca aaaaataacg
       61 tccattccgg acatcggtgc cttggccagc tccagcacat tgagccgggc gcaacacaca
      121 cgcgcggcca gattcggcac cactttgaaa cgtccatcat cctgagccag gaagtaacgc
      181 tggcacaggt cggcatccaa ctgttccagg cgccgcgcgc catattgacc atcgcgcgcc
      241 ttgctcaggg cattcaggct gatatcggtg cctgttaccc cgaaatgatc cggatgttca
      301 ctttcacgca gaacctcggc cgcactgatg gccagcgaat aaggctcttc gccactggca
      361 cagcccacgc tccacagctc ccatggctgc gtcacgccct gctgcaaacg ctcgcgcaaa
      421 tagctttcga gcacttcaaa ggaaggacga tgacgaaaga aacgggtttc ttgcacagtc
      481 agccgatcca gcagggtcga ccattccacc gcaccgcggg gaccatcggt tacctgccgg
      541 taatacgtgg cgtaatccat tacgcccagc tcgcgcatgc gcgcactcag attggtttgc
      601 agaaacgccc gccgccgttc attgagaacg accccggtac gctcctcgag caacgtctgc
      661 cagtcacgaa actccgtggc tgtcatgtct gccagaggct gcaaggccca gacgcctgac
      721 tgcatgtcgt gcccctcgct cacggccata gtccttgtgc ggcaggcctt tcagcttgcc
      781 gcgcagcacc tgattaagcc tgttccccac ccggcaaggt aaagccggat accgacttac
      841 gcatctcact ggccattttg gccaggttac caatgctctt ggcggtggca gtggtaccgg
      901 aggaggtttg cgaggtgatt tcctggatca cgttcatcgt gttggaaatg tggcccgccg
      961 acgaagcctg ctggcgagca gcgttggaga tgttctgaat caaggccgca agggttttcg
     1021 ataccttctc gatctcttcc agtgccaccc cggcgtcctg cgccagacga gcaccgcgca
     1081 ccacttcgga agtcgtttgt tccatcgaga taacggcttc gttggtgtcg gtttgaatgg
     1141 tcttaaccag tgcctcgatc tgcttggttg cagccgacga acgttctgca aggcgttgta
     1201 cttcgtccgc taccacggcg aagcctcggc ccgcgtcacc ggccatggag gcctggatcg
     1261 cggcgttcag tgcgaggatg ttggtctggt cagcaatgtc gttaatcagg ctaacgatgt
     1321 caccgatctc ctgggacgat tcaccgaggc gtttgatccg cttcgaggtg tcctggatct
     1381 gctcacggat gttatccatg ccggtaatgg tgttgtgcac cacttcgttg cctttgttgg
     1441 cgatggctac ggaacgttcc gctaccgcgg aggactccga ggcgttcgcc gatacctggt
     1501 caatcgacac ggccatttcg ttgatcgccg cagaagcacc ggcaatttcc tgggcctggt
     1561 gctcggacgc ttcagccagg tgcatcgccg tggcctgggt ttcctgggcc gcaccggcta
     1621 cctgaacggc ggtcaggttg atcgttgcta ccaggtcacg cagctggtcg atggagtagt
     1681 tgatggagtc agcgatggca ccggtgaagt cttcggttac cgtcgcggct acggtcaagt
     1741 caccgtctgc gaggtcggca atttcgtcga gcagtcgcag aatagctgcc tggttacgtt
     1801 cgttcttctc ggcggtagac gccagacgac ggttggtttc acgcaccatc accaggccga
     1861 tcagaatgat cgaaccaagc gccagggcac caagcacata accgatcagg gtattgatag
     1921 cacgaccggt ggccaggtta tcgaaaccga cagcaagatc tgaagccttg tccagcaaag
     1981 tctgcgaaac gctgaagatc acgttggctg actcacgcac ctggaacaac tcaggcgagg
     2041 tttcgagaat ctcgtccacc gaaccggata cgaattcgaa cagttcggaa atttccgcca
     2101 gacgctcgag ggcttcctcg tcgcttacct gggtaatttc catggcggcg ttgccttcga
     2161 gcatcgcgct caatacgcga ccgaacaggc tggcgtcgcg accgaacatg tcggcggctt
     2221 gcaccgagtc ctggtcaccg gcgagtacct tgtttaccga accgaggata cgttctgcca
     2281 gcaaggactg acgctgagca accgagacct gagccgccgg cgcgccgctt tccagtagaa
     2341 tgtcgaccac ttcttcgtac tcgacctgca gctgcggaat ggtttccgcc agggtagcgg
     2401 ctacctggtg cagcgacagt acggtctgtt cgttggccag gatggcgtcg gtgttctgcc
     2461 gcagcgtatc ccagtcctgc tgcaccacag cgatctgtgg ctgcagggat tccggcgctg
     2521 cgggcagccc ggtctcctcg ttaccgtcgg tcagataacc ccagcgggtc tggaagtcgt
     2581 tacgcgcatc gcgcagcaag gcgaacgctt cagccgtacc agccgccgct tccgtggagt
     2641 ttttcgcgat acgctgggac agtacgcgca gctcaccggc gtggccgatg tactccgtgt
     2701 cgtagttgga ttgggtgttg atgtaggcga agttggcgaa caacaacacg atcgagacaa
     2761 tcaggacaat aaacagccct gcgatcaatg tgctactgcg cgcacctgct aacaaattac
     2821 ctgcatttag ttttttcatt tactggcccc cgcctggacc gaccgcacta cggttcccat
     2881 gtaacgccaa aaggccggta aagccgaccc caccgataaa ctcttaatac gccacgtcga
     2941 gaaaacccgg gttctgggcc agcgcgtgtg ggctgaacac caaccagggc tgttcacgtt
     3001 gaaatactcc gtggataaaa ggtgctatgg acgcctcgag cggcggcagc tgctcggaga
     3061 aggtgtccac aggaaaatgc tgcatgccga acacttcatc gactgtaagg ccggcaaaga
     3121 cttcttggtg gtcaaccacc agcactcggc gttttttgcg taacggcgac agctcattgc
     3181 cgaagaaacc gcacaggtcc atcacaggca gcaagcgacc acgtacgttg gccacgccct
     3241 tgacccagct tttcacgcct ggcagcagcg tatgtcgcgg ctcaggcaac acctcgccaa
     3301 cctcgcccat gggcgcgacg aaaaaacgtt cgcccatgcg aaagccgata ccgctccagg
     3361 tctgtaccac cgcttgctgc gaaggcaagc cggcagcaag cgaacgacag agcctgtcga
     3421 tatcctgcag ggtttgaaaa ggtgtttgcg cgtccgacat gccagctgcg gccttattct
     3481 tgtttaattg cggactgaac cggttttagc cggccaagac cgcattcaag gttttaacca
     3541 gggtttcttc atcaaccggc ttggtcaggt aatccttcgc accctgacgc ttgccccaga
     3601 ccttgtcggt ttcctgatcc ttggtggtga cgataatcac cgggatgtga ctagtgtcag
     3661 catccttggt cagctgacgg gtcgcttgga agccgttgag gccgggcatg acgatatcca
     3721 tcagcacagc gtccggcttt tcctggcgtg ccagggcaac gccgtcggca ccattttccg
     3781 ctttcagtac ctgatggccg tgcttttcca gcatggcggt cagcttgtac atttcagtcg
     3841 gcgagtcatc aacaatcaga attcgagcca tggtgtttcc caatacttta aaaaggcgct
     3901 cagctgttca gccaggcgtc aggacgcttg ctccaccggt acaaagtcag gcacatgggc
     3961 tttgatcgca ccgagcagct cttctttact gaagggcttg gtcaggtatt gatcggaacc
     4021 gacaatgcgc cccttggctt tgtcgaacaa accgtctttg gaagacagca tgatcaccgg
     4081 ggtggattta aaagcgctgt tgttcttgat caaggcacag gtctgatagc catcgagccg
     4141 cggcatcata atgtcgacaa agatgatgtt cggatgggta tcggcgatct ttgccaaagc
     4201 atcgaagccg tcaactgcag taattacttc gcagcccacc tttttcagca gggtttccgc
     4261 ggtacgacga atcgttttcg aatcgtcgat caccatcacc ctcaaaccct cggaatgctg
     4321 ttccatttct gccctaccat cgcctcggtg agtcgttatt tttgcgttat atcagtgcgc
     4381 ctgaggccct gtcaccgacg ggctacaacc caatcgtcgg gcctttttag cacactctcc
     4441 agatgcaatc tataagccac gcgggggcca aggtttttcc ttgaccaccc gacttgtcag
     4501 cgccaccctg acgccacatc acgtcttagc acactttaat cgctgccatt ttacggagaa
     4561 caccgccatg agcgtccgcc tcggcattgt catggacccc atcg
//
LOCUS       DASRUM010000270         8132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2462954, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000270 DASRUM010000000
VERSION     DASRUM010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2462954"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..904)
                     /locus_tag="VFF22_18155"
     CDS             complement(188..904)
                     /locus_tag="VFF22_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011060279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HZX18779.1"
                     /translation="MTVIYGHRGAKGEVPENTLASFQQCLKHGVRRCELDLHLSRDGE
                     LMVIHDPTLKRTTGHRGKVVEHDAAELVTYDARKGGPGAVTPCPIPRLEELFQHCDFE
                     HWQLEVKSASKVRSARMVKAIAELAERYKLTDKVTVTSSSREVLGALQRLTPHLQRGL
                     VAEYAWLDPLKVAKHYGCHLLALNWTLCTPERLLKAQKAGLHVSVWTVNEPALMRRLT
                     DFGVDSIITDFPGLAVATLE"
     gene            complement(901..1203)
                     /locus_tag="VFF22_18160"
     CDS             complement(901..1203)
                     /locus_tag="VFF22_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HZX18780.1"
                     /translation="MPRLFLLLALFLPLGAVADTLQIPIGQQGGSTLQLPQFGESQRA
                     VLERFGLADEEHPSVGNPPITRWDYREFSVYFEHKHVINSVRHHQPGAALPDKEQQ"
     gene            complement(1216..1797)
                     /locus_tag="VFF22_18165"
     CDS             complement(1216..1797)
                     /locus_tag="VFF22_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZX18781.1"
                     /translation="MPTQDEDDRREYYRIDDTIALEFTLLSGAEALASDELHDSSPLF
                     NLLSDLHLMDFESQHLLRHITERDRTLANYLKVMNKRIDLLGQAVAQSLLRDIGTPRK
                     VSLSEGGVSFNNSQPVATGAHLAVKMVLMPQALGLLLRAKVVHCQPLPSQQFEIGAEF
                     EALSDAQRQLLARHILQRQALERRQAREQPLQP"
     gene            1891..3141
                     /locus_tag="VFF22_18170"
     CDS             1891..3141
                     /locus_tag="VFF22_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HZX18782.1"
                     /translation="MFRPLFVFIGTRYTRAKRRNHFVSFISLTSMIGLALGVLVMILV
                     LSVMNGFDHEMRTRVLGMVPHATVESATPIKDWRSLADKAQSNPQVLAVAPFSQMQGL
                     LTNDGKVQKVLINAVDPLEEAKVSIIGNFFQQGRLDALLPGEFGMVLGDKAAAKLGVG
                     LGDKVTFVAPEVTVTPAGMFPRLKRFTVVGIFHVGAGEIDGHLALTHVQDLARLQRWQ
                     VDQVQGIRLKFADLFEAPRTAWALAQKLGEGDYYARDWTRTHGNLYQAIRMEKAMIGL
                     LLLLIVAVAAFNIISTLVMVVTDKKGDIAILRTLGATPRQIMAIFMVQGTVIGVFGTA
                     IGAVLGCLAALNISSAIAALEGLLGIKFLNADVYFIDYLPSQLMLADVLLVCGAALIL
                     SFFATLYPAWRAARTQPAEALRYE"
     gene            3164..3847
                     /gene="lolD"
                     /locus_tag="VFF22_18175"
     CDS             3164..3847
                     /gene="lolD"
                     /locus_tag="VFF22_18175"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HZX18783.1"
                     /translation="MQDRAVLSCRNLGKSYDEGPQSVVVLQDLQLELHPGERVAIVGS
                     SGSGKSTLLNMLGGLDTPSTGSVWLAGEELSALNEKQRGLLRNRALGFVYQFHHLLPE
                     FTALENVCMPLLIGRTPISEARQRATALLERVGLGHRLAHKPSELSGGERQRVAIARA
                     LVNQPGLVLLDEPTGNLDHHTAQGIQDLMKELSSSSQTAFLVVTHDMALAQQMDRILR
                     LEDGKLVAA"
     gene            3847..5091
                     /locus_tag="VFF22_18180"
     CDS             3847..5091
                     /locus_tag="VFF22_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005771340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HZX18784.1"
                     /translation="MFRPLSIFIGIRYTGAKRRNHFISFISMTSMIGLALGVLAMIVV
                     LSVMNGFQKEMSTRILGMVPHASIAGTQPLDNWQQVAAAAKKNPQVVAAVPFAELEGM
                     LSVRGAMQPVQLHGVDPALEPQVSIIDKHMQQGRLSDLREGEFGVVLGEITARRFQLR
                     VGDKLALIVPELSSVPGGISPRMQRLNVVGIFKVGAELDSSLALIHVADAAHIQRMQP
                     GRVQSVRLKLQDLYQAPQVAAQIVSELGAGYSSADWTLTQGSLFSAMKMEKTMIGLLL
                     LLIVAVAAFNIIATLIMVVADKGADIAILRTLGATPGQIMGIFMVQGTVIGVIGTVIG
                     AVLGILAALNVSQLVGWIERLSGQQVMSSDVYFISNLPSELLLSDVLLICGAAFSLSF
                     LATVYPSWRAAQIQPADALRYE"
     gene            complement(5143..5676)
                     /locus_tag="VFF22_18185"
     CDS             complement(5143..5676)
                     /locus_tag="VFF22_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HZX18785.1"
                     /translation="MPRRLFKRYMPHPDRIKGNKSLRFLGSLIHDPNLWHLNRHSVSR
                     AMAIGLFWAMIPMPMQMAAAAAVAIPARGNLPISIGLVWLTNPITMPPVFYCSYKFGA
                     WLMNTPTLQMPDHITLGWVGHVLQTHWQPLYLGSFVLGLLFALIGYFATNAYWRWWVR
                     HNWRKRQEKRRQQDAQH"
     gene            5811..8036
                     /locus_tag="VFF22_18190"
     CDS             5811..8036
                     /locus_tag="VFF22_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HZX18786.1"
                     /translation="MRTGIFALVAGLLVLRFLPALPPLWLLALLPVVGLMLLPFRSYP
                     IALFLFGFSWACVSAQWALDDRLARQLDARTLWLQGQVVGLPEVDEGVVRFQLEQVAS
                     RRDKLPARLRLAWYGGPTVRAGETWRLAVRLKRPHGLVNPQTFDYEAWLLAQRIGATG
                     TVKSGELIAPASGSGAWRDRLRQQLLATPAYGREGALAALVLGDDSGLSTADWQVLQN
                     TGTVHLMVISGQHIGLLATLLYGLVAGLARLGFWPRSWPWLPCACVLAFASALAYGLL
                     AGFEVPVQRACVMVGLVLLWRWRFRHLGVWLPLLLALLVVLLGEPLASLQPGFWLSFG
                     AVMLLILIFSGRLGAWPWWSTLLRAQWAMAIGLLPLLLALGLPVSASGPLANLVAVPV
                     VGLLVVPLALLGTLLLPVPGVGAALLWIAGGVLDLLFELLMLLSLWLPAWLPSVLPFW
                     AWLSVALGALVLLLPAALPMRSLGALLLLPLLFSPQQRPEYGRAEVWMLDVGQGLAVL
                     VRTQKQDLLYDAGPRFGEFDTGERVVLPSLRALDVRGLDLLLISHADNDHAGGAAAIK
                     RGLPVRQVLSGEPDKLAPSLQAQACVSGQHWQWDGVQFSTWRWTDATDGNQASCVLRI
                     EANGERLWLTGDIDSHAERALLNSGLAMPAQWLLAPHHGSRSSSSPALLAQVRPKAAL
                     VSRGAHNAFGHPHAEVVERYRARQARLYDTALHGAVRIRLGALEQAQGLRYRARFWRE
                     K"
BASE COUNT         1373 a         2256 c         2710 g         1793 t
ORIGIN      
        1 gttcgacggc ctcaaccggc ttttttgagc ggctccggcc ggtggcctga gccggccggg
       61 gagaacccgt tcagcgactc cagcgactgg cgttggccta atcgcgaaag tttctgatca
      121 ggcttaacga aaaaccggcc catgtggccg gtttttcgtt tctctgtagc tgggtcgccc
      181 gggatggtta ttccagggtc gccacggcca ggccgggaaa gtcagtgatg atgctgtcta
      241 caccgaagtc cgtcaggcgg cgcatcagtg ccggttcgtt gaccgtccac accgagacat
      301 gcaaaccggc tttctgcgcc ttaagcaggc gctctggggt gcacagcgtc cagttcagcg
      361 cgagcaagtg gcagccgtaa tgcttggcta ctttcaatgg gtcgagccag gcgtattcgg
      421 cgaccaggcc gcgttgcagg tgcggcgtca ggcgttgcag agcgccgagc acttcgcggg
      481 agctggaggt tacggtgacc ttgtcagtca gcttgtagcg ctcggccagc tcggcaatcg
      541 ccttgaccat gcgtgcggag cgaactttcg aggcgctttt gacttccagc tgccagtgct
      601 caaaatcgca gtgctggaac agctcttcca gccgtgggat cgggcagggc gtaacggcgc
      661 cagggccgcc tttgcgtgcg tcataggtca ccagctcggc ggcgtcatgc tcgactacct
      721 tgccgcggtg gccggtggtg cgcttgaggg tcgggtcgtg aatcaccatc agctctccat
      781 cgcgcgacag gtgcagatcc agctcgcagc ggcgcacgcc atgcttcagg cattgctgga
      841 agctggccag ggtgttttcc ggcacctcgc ctttggcgcc gcgatgtcca tagatgactg
      901 tcactgttgc tccttgtcgg gcagggctgc gccgggctga tgatggcgca cgctattgat
      961 cacgtgttta tgctcgaagt agacgctgaa ctcgcggtaa tcccagcgag tgatcggcgg
     1021 gttaccaacg ctaggatgtt cttcatcggc caggccgaaa cgctcgagca ccgcgcgctg
     1081 ggattcgccg aactgcggca gctgtagtgt gctgccacct tgctggccga taggaatttg
     1141 cagggtatcg gcgacagcgc cgagaggcag gaacagggcg agaagcagaa acagacgggg
     1201 catggtggct cgctatcagg gttgcagagg ttgctcgcgg gcctggcgac gttccagggc
     1261 ctggcgctgc agaatatgcc gggcaagtag ctggcgctgg gcatcgctca gggcctcgaa
     1321 ttctgcacca atctcaaatt gctgcgaggg cagtggttgg caatgcacca ccttggcccg
     1381 caatagcagg ccgagggcct gcggcatcag caccatcttc accgccaggt gtgcgccggt
     1441 ggccacgggt tgagagttat tgaagctgac gccaccctcg gacaggctga ccttgcgcgg
     1501 cgtgccgatg tcacgcagca ggctttgcgc cacagcctgg ccgagcagat cgatgcgctt
     1561 gttcattacc ttgaggtaat tggccagggt gcgatcacgt tcggtgatgt gccgcagcag
     1621 atgctgcgac tcgaagtcca tcaggtgcag atcgctgagc aggttgaaca gcggcgagct
     1681 gtcgtgaagt tcatcactgg ccagggcttc tgcgcccgag agcagggtga attccagtgc
     1741 gatcgtatcg tcgatacgat agtattcgcg gcgatcatct tcatcttgag tcggcatggc
     1801 gaacccatgg cagcagtagt ggtctgagtg taaagctgct ggccaggccc cgccacaagg
     1861 acgttcttct ttacctgcaa aagcccccac atgttcagac ctctgttcgt atttatcggc
     1921 acgcgctaca cgcgggccaa gcgtcgcaac cacttcgtat cctttatttc cctgacctcg
     1981 atgatcgggc tggcactggg tgtgctggtg atgattctgg tgctgtcggt gatgaacggc
     2041 ttcgaccatg aaatgcgcac ccgcgtgctc ggcatggtgc cgcacgccac ggtcgagagc
     2101 gctacgccga tcaaggattg gcgcagcctg gccgacaaag cgcagagcaa cccgcaggtg
     2161 ctcgcggttg cgccctttag ccagatgcag ggcctgctga ccaacgatgg caaggtgcag
     2221 aaagtgctga tcaatgcggt cgatccgctt gaagaagcca aggtctcgat catcggcaat
     2281 ttctttcagc agggccgtct ggatgcactg ttgcctggtg agtttggcat ggtgctgggt
     2341 gacaaggcag ccgccaaact cggcgtcggc ctgggtgaca aggtcacctt tgtcgcgcca
     2401 gaagtgacgg tcacccctgc cggcatgttt ccgcgtctca agcgttttac cgtggttggc
     2461 attttccatg ttggcgcagg cgagattgac ggtcacttgg ccctgaccca tgtgcaggat
     2521 ctggcgcgct tacagcgctg gcaggtcgat caagtgcagg gtattcgcct gaaattcgct
     2581 gacctgttcg aagccccgcg caccgcttgg gcgctggcgc agaagttggg tgagggcgat
     2641 tactacgccc gcgactggac ccgtacccat ggcaacctgt accaggcgat ccgcatggag
     2701 aaggcgatga ttggcctgct actgttgctg atcgtcgcgg tggcagcgtt caacatcatc
     2761 tccaccctgg tcatggtggt taccgataag aagggcgata tcgccatcct gcgcaccctc
     2821 ggcgccacgc cgcggcagat catggcgatc tttatggtcc agggcacggt gatcggcgta
     2881 ttcggcacgg ccatcggcgc ggtgctgggc tgccttgccg ccttgaatat cagcagtgcc
     2941 attgccgcgc tggaagggct gctggggatc aagttcctca atgccgatgt gtatttcatc
     3001 gactacctgc catcgcagtt gatgctggcg gacgtgctgc tggtctgcgg cgcggcattg
     3061 attctaagtt tcttcgccac cctgtatccg gcctggcgcg cggcgcgtac ccagcctgcg
     3121 gaggcgctac gttatgagtg atcaggttat gagtgatccc gctatgcaag atcgtgcagt
     3181 tcttagttgt cgcaacctcg gtaaaagcta cgacgagggc ccgcaatcgg tggtggtgct
     3241 gcaggacctg cagctggaac tgcaccccgg cgagcgcgtg gcgattgttg gtagctccgg
     3301 ctcgggcaag agcaccttgc tgaatatgct cggcggcctg gatacgccga gcaccggcag
     3361 cgtctggctt gcgggcgagg agttgtcggc gctgaatgaa aagcagcgcg gtctgctgcg
     3421 taaccgtgcc ctgggtttcg tttaccagtt tcaccacctg ctgccggagt tcaccgcgct
     3481 ggagaacgtc tgcatgccgc tgctgattgg ccgcacgccg atcagtgaag cgcgtcaacg
     3541 cgccacggcg ctgctggaac gtgttggcct cggccatcgc ctggcgcaca aaccgtctga
     3601 gctgtccggc ggtgagcgtc agcgtgtggc catcgctcgc gccctggtca atcagcctgg
     3661 tctggtgttg ctcgatgagc ccaccggcaa cctcgatcac cacaccgcgc aaggcattca
     3721 ggacctgatg aaggagctga gcagctcgtc gcaaaccgcc tttctggtgg tgactcacga
     3781 catggccttg gcccagcaga tggaccgcat cctgcgtctg gaagacggca agctggtggc
     3841 tgcctgatgt ttcgtccgct gagtatcttt atcggtatcc gctataccgg ggccaagcgg
     3901 cgcaaccact ttatttcctt tatctcgatg acctcgatga tcggcctggc cttgggcgtg
     3961 ttggccatga tcgtggtgct gtcggtgatg aacgggttcc agaaggaaat gagcacgcgc
     4021 attctcggta tggtgccgca tgccagcatc gccgggacgc agccgttgga taactggcag
     4081 caggtcgctg ctgcagcaaa aaagaatccg caagtagtcg cggcagtgcc gtttgccgaa
     4141 ctcgaaggca tgctttcggt acgcggggcg atgcagccgg tgcagttgca cggcgtcgat
     4201 ccggcgctgg aaccacaggt atcgatcatc gacaaacata tgcagcaagg ccgcctgagc
     4261 gatttgcgcg agggtgagtt cggcgtggtg ctcggcgaga ttaccgcgcg gcgcttccag
     4321 ctgcgggtgg gcgacaagct ggcgctgatc gtgccggagc tgagtagcgt gccgggcggt
     4381 atcagtccac gcatgcagcg ccttaacgtg gtaggcatct tcaaggtggg cgccgagctg
     4441 gatagctcgc tggcgttgat tcacgttgct gatgcggcgc atatccagcg catgcagccg
     4501 ggccgggtgc agagcgtgcg gctgaaactg caggatctgt atcaagcacc gcaagtggcc
     4561 gcgcagattg tcagtgaact gggtgcgggt tacagctccg cagactggac cctgacccag
     4621 ggcagcctgt tcagtgcgat gaaaatggaa aagaccatga tcggtctgtt gctgctgctg
     4681 atcgtcgcgg tggcggcctt caatatcatt gcaacgctga tcatggtggt ggcggacaaa
     4741 ggcgcggata tcgccatcct gcgcactctg ggcgcgacac cagggcagat catgggcatc
     4801 tttatggtgc aaggcacggt gatcggggtg atcggcaccg taatcggcgc ggtgttgggt
     4861 attctcgcgg cgctgaatgt cagtcagctg gttggctgga ttgagcgttt gagcggtcag
     4921 caggtgatga gctcggatgt gtatttcatc agcaacctgc catctgagct gctgctttca
     4981 gacgtgctgc tgatctgcgg ggcggcgttc agcctgagct ttctggctac cgtctacccg
     5041 tcctggcggg ctgcgcagat tcagccggcc gatgcgctac gttacgaata agctacgctg
     5101 gcagaaacaa aaaagccgtt actgagtaac ggcttttttg tgtcagtgct gcgcgtcttg
     5161 ctgacggcgt ttttcctgac gtttgcgcca gttgtggcgc acccaccagc gccagtaagc
     5221 gttggtggcg aagtagccga tcaaggcgaa cagcaggccg agtacgaacg agcccagata
     5281 cagcggctgc cagtgggttt gcagaacatg gccaacccag ccgagagtta tgtgatcagg
     5341 catttgcaga gtgggtgtgt tcatcagcca ggcgccgaac ttgtagctgc agtagaacac
     5401 tggcggcatg gtgatcgggt tggtcagcca gaccaggcca atcgaaatcg gtaggttgcc
     5461 tcgggccggt atggccactg cggcggcagc ggccatttgc atgggcatgg gaatcatcgc
     5521 ccagaacagg ccgatggcca tggcacgtga aacagaatgt cggttgaggt gccagaggtt
     5581 ggggtcgtgg atcagactgc ccagaaagcg cagggattta ttgcccttga tgcggtctgg
     5641 gtgaggcatg tagcgcttga ataaacgacg cggcatgaag gctctctggc gggtaaaagc
     5701 gccgacatta tgcacccttt gatgacagcc tgctttcaca ctttgtgaca gcaaataagc
     5761 aaaagctgat cgaccggtta aatgctgtat tcgactcatg gagcgagtct atgcgcacag
     5821 ggattttcgc gctggtggct gggctgctgg tgctgcgctt tcttccggct ttgccgccgt
     5881 tgtggctgtt ggctcttttg ccggttgtcg gtttgatgct gctgccgttt cgcagttatc
     5941 ccatcgcctt gtttttattc ggtttcagct gggcttgtgt gtcggcacag tgggcgctgg
     6001 atgatcgcct ggcgcggcag ttggacgcgc gtaccttgtg gttgcaaggg caggtggttg
     6061 gcctgcccga ggtggacgag ggcgtggtgc gctttcagct ggagcaggtg gcttcacgtc
     6121 gcgacaaatt gcccgcgcgt ctgcgcctgg cctggtatgg cggaccaaca gtgcgcgcgg
     6181 gcgagacctg gcgcctggcg gtacggctca agcgccccca tggtctggtc aatcctcaga
     6241 cattcgatta cgaagcctgg ctgctggcgc agcgcattgg tgctacgggc acagtaaaaa
     6301 gcggcgagct gattgccccc gccagtggct ccggggcctg gcgtgatcgc ttgcgtcagc
     6361 agctcttggc aacgcctgca tatggtcgcg aaggtgcgtt ggcggctttg gtgctgggtg
     6421 atgattcggg gctgtcgacg gcggattggc aggtgctgca gaacaccggt acggtgcatc
     6481 tgatggtcat ctccggccag catatcggcc tgttggcgac acttttatat ggtctggttg
     6541 ctggcttggc gcgtctgggg ttctggccac gcagctggcc ctggctgccc tgcgcctgtg
     6601 tgctggcctt tgccagcgcg ttggcctacg gcctgctggc aggcttcgag gtgccggtgc
     6661 agcgcgcctg cgtgatggtc ggcctggtgc tgctctggcg ctggcgtttt cgccatctgg
     6721 gcgtgtggct gcctttattg ctggcattgc ttgtggtgct gctgggtgag ccgctggcaa
     6781 gtttgcagcc ggggttttgg ttgtcctttg gcgcggtgat gctgttgatc ctgatcttca
     6841 gcggccgcct gggtgcctgg ccctggtgga gcaccctgct gcgcgcgcaa tgggccatgg
     6901 ctatcggctt gttgccgctg ctgctggctc tcggtctacc tgtgagtgcc agtggcccct
     6961 tggccaacct ggtagcggtt cccgtggttg gattactggt agtgccgctg gcattactcg
     7021 gtacgttatt gctgcccgtg cctggggttg gtgctgcgct gctctggata gcaggcgggg
     7081 tgctggacct gctgttcgag ttgctgatgc tgttgtcctt gtggcttccg gcctggttgc
     7141 ccagcgtctt gccattctgg gcctggctgt cggtggcgct gggcgcattg gttttgctac
     7201 tgccagccgc tttaccaatg cgcagcttgg gtgcgttatt gctgctgccc ttgttattta
     7261 gcccgcagca gcgtcctgaa tacgggcgag ctgaggtgtg gatgctagat gttggtcagg
     7321 gccttgcagt actggtgcgc acccagaagc aagacttgct gtatgacgcc gggccgcgct
     7381 ttggcgagtt tgataccggt gagcgggtgg tgctgccatc gctgcgcgca ttggatgtgc
     7441 gtgggctcga tctgctgctg atcagtcacg ccgataacga tcacgccggt ggcgccgcag
     7501 cgatcaagcg cggtctgccg gtcaggcagg tactcagtgg cgaaccggat aagcttgcgc
     7561 cttcactaca ggcccaagct tgcgtcagtg gccagcactg gcagtgggac ggcgtgcaat
     7621 tcagtacctg gcgctggacg gacgcgaccg atggcaatca ggcgtcctgc gtgctgcgta
     7681 tcgaggccaa tggtgagcgg ctgtggctga ccggtgatat cgacagccat gccgagcggg
     7741 cactgctcaa tagcggtctg gcaatgccgg cgcagtggtt gctcgcgccg caccatggca
     7801 gtcgcagttc atcctcgcct gccttgctgg cgcaggtcag acctaaagct gcgttggttt
     7861 cccgtggcgc acacaatgcc tttggccatc cgcatgccga ggttgtcgag cgttaccggg
     7921 cgcggcaggc acggctgtac gacacggccc tgcacggcgc ggtacggatt cgtttgggag
     7981 cgctcgagca ggcgcagggt ttgcgctatc gagcacgttt ttggcgggaa aaatgagaac
     8041 ggcggcggtc ggcgaggccg cgaccctatg ctagagtggc gccacttttg tgaaggggat
     8101 ctgccaccgt gtgggaactg gttaaagctg gc
//
LOCUS       DASRUM010000271        13167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_794413, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000271 DASRUM010000000
VERSION     DASRUM010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 13167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13167
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794413"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..754
                     /locus_tag="VFF22_18195"
     CDS             32..754
                     /locus_tag="VFF22_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZX18787.1"
                     /translation="MQADASEQQPAAEKATEAPGKTLLLEAALRLTSTSRSLSNLGLR
                     ELAREAGLNPNTFYRHFKDVDDLGLTVIRQISTQLRQPLRDLRREAAERAVQSTQASS
                     PMLFGIDLQRGRRVCHETVQLFFDFVAQNPEAFILGVRELHGASAVLRAALQEVMDEF
                     GADMAEDIIAFKLLPEMVNPAVVLQVSNLISRNLFQLSLDYIGQAERQAAICALAEEQ
                     IVMLFTGASVLQGLGQLKLPVS"
     gene            777..1175
                     /locus_tag="VFF22_18200"
     CDS             777..1175
                     /locus_tag="VFF22_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZX18788.1"
                     /translation="MVALPLFGGCACGNVRYRLESLPGDSGYCHCRTCQKIGATPALP
                     WAMVPATALHYLSGDIGVFHSSARGERRFCRDCGTPLEYREQGGEEVGLNSVTLDHPE
                     WFPPQKHIWYASNLPWFELADTLPRFARES"
     gene            complement(1211..2041)
                     /locus_tag="VFF22_18205"
     CDS             complement(1211..2041)
                     /locus_tag="VFF22_18205"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZX18789.1"
                     /translation="MTAKTSTLTASFPVRRMDFSFDESQKFWFAGDQFMSHFMNNLSS
                     LFPYGEKFFVDSVRAVRDQVTEPQLKKDISAFIGQEAMHSKEHAAYNDYAAEHNIDLE
                     ILELRIKVLLEWVTKFSTKKHRLAATCALEHFTATMAEQLLQREDLTSQMNDPKLYQL
                     WMWHAIEENEHKAVCYDVYQQVYGGYFTRTIMMAITTVIFLGVIGWFQVHLLRKDGQL
                     FNWRSWGKGLKTLFGPRNGFLTKLLLPYLDYYRPGFHPLDHDSKALEQRWRERLGFNS
                     "
     gene            complement(2359..3174)
                     /locus_tag="VFF22_18210"
     CDS             complement(2359..3174)
                     /locus_tag="VFF22_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HZX18790.1"
                     /translation="MNEISMNPTSSHWRLRLGALMESKIITRLVTTLIVLNAAILGIQ
                     TYPGLMADWGELLLALDQLILAVFILELALRFVARGLGLLRDPWAVFDCLVVGVALVP
                     SSGPFAVLRALRVLRVLRLISINPNMRRVVEALLSSLPGMGSIAMLLGLVFYVAAVMA
                     TQLFGAAFPEWFGSLGASLYTLFQVMTLESWSMGIVRPVMEQFPLAWMYFLPFILIAT
                     FMMLNLFIAVVVNAMQTTHEQEAKLAPPSPTEQLLLDELRALRAEVAELRQQR"
     gene            complement(3273..3821)
                     /locus_tag="VFF22_18215"
     CDS             complement(3273..3821)
                     /locus_tag="VFF22_18215"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HZX18791.1"
                     /translation="MIFDEFTIKTLIRPVVDFPKPGVIFRDITPLFHSPRALRMVVDS
                     FIQRYVEADFSHIGAMDARGFLIGSIIAYELNKPLILFRKQGKLPADVLSESYQTEYG
                     EAKLEVHADSLCEGDKVLIFDDLIATGGTLLAAAQLVRRMGASIIEAAAIIDLPELGG
                     SQKLQDSGIPTFALTSFALTDQ"
     gene            complement(3850..4584)
                     /gene="fnr"
                     /locus_tag="VFF22_18220"
     CDS             complement(3850..4584)
                     /gene="fnr"
                     /locus_tag="VFF22_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HZX18792.1"
                     /translation="MSESIKLHTPHQAHCKDCSLASLCLPLSLNLEDMDALDEIVKRG
                     RPLKKGEFLFRQGDTFNSVFAVRSGALKTFSLSDSGDEQITGFHLPSELVGLSGMDSE
                     AYPVSAMALETTSVCEIPFERLDELSIQLPQLRRQLMRIMSREIRDDQQMMLLLSKKT
                     ADERIATFLVNLSARFRARGFSANQFRLAMSRNEIGNYLGLAVETVSRVFTRFQQSKL
                     LEAEGKEVHILDPIELCALAGGNLEG"
     gene            4740..5204
                     /locus_tag="VFF22_18225"
     CDS             4740..5204
                     /locus_tag="VFF22_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18793.1"
                     /translation="MRLESTQLSTRMNLALESRSAATEGVAASREEEKQVLRDSLRVS
                     LSELGKARSAAAQKNKDIDESSLPDVLKDILKMIRELKAQIAAKKAELEEVMSDQSLD
                     PEAKRIKIEMLRGELASLQGALSSANATLIKVMREQNLSDQQMQEVSSLLMK"
     gene            complement(5210..6592)
                     /gene="hemN"
                     /locus_tag="VFF22_18230"
     CDS             complement(5210..6592)
                     /gene="hemN"
                     /locus_tag="VFF22_18230"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HZX18794.1"
                     /translation="MLNAIQWDSSLIRRYDQAGPRYTSYPTAVQFHERVSQFDLLHAL
                     RESRKALRPLSLYVHIPFCAHICYYCACNKVITKDRGRALPYLEKLQREIEIIGRHLD
                     PQQKVEQLHFGGGTPTFLSHDELRHLMGHLRKHFNLLDDDSGDYSIEIDPREADWSTM
                     GLLRELGFNRVSLGVQDLDPVVQRAVNRLQTLEETRAIIEAARTLQFRSVNIDLIYGL
                     PLQTPERFARTVAEVIALQPDRLSLFNYAHLPERFMPQRRINTSDLPNPGDKLAMLQA
                     SIEQLSAAGYRYIGMDHFALPDDELASAQEDGSLQRNFQGYTTHGHCDLIGLGVSSIS
                     QIGDLYCQNNSDLSKYQDSLGHSELATLRGLHCDADDRLRRAVIQQLICHFQLRFADI
                     EQAYAIDFRSYFAEVWPQLQQMASDGLIELTAQGIEVRPAGRLLVRSLCMLFDRYLSE
                     LNQQRFSRVI"
     gene            complement(6684..7367)
                     /locus_tag="VFF22_18235"
     CDS             complement(6684..7367)
                     /locus_tag="VFF22_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZX18795.1"
                     /translation="MLELLPLLVSALILGLLGGGHCLGMCGGLMGALTLAIPPEQRAQ
                     RFKLLLAYNLGRIFSYSVAGLLLGLAGWAVANSPLVMGLRVVAALLLIAMGLYLAGWW
                     SGLTRIEALGRGLWRYIQPLTRRFMPVTSLPRALVLGSLWGWLPCGLVYSTLLWAASQ
                     GNAVDSAALMLAFGLGTLPVLLATGMAAERLTGLLRKQGVRMAGGILVILFGLWTLPG
                     PHQHWLMGH"
     gene            complement(7360..7578)
                     /gene="ccoS"
                     /locus_tag="VFF22_18240"
     CDS             complement(7360..7578)
                     /gene="ccoS"
                     /locus_tag="VFF22_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HZX18796.1"
                     /translation="MPALYILIPVAIALVGFAIWLFFWAVDSGQYDDLDGPAHSILFD
                     DEDPKHQAAVSQVQEEEQSKESGDKPGA"
     gene            complement(7618..10026)
                     /locus_tag="VFF22_18245"
     CDS             complement(7618..10026)
                     /locus_tag="VFF22_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003132982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZX18797.1"
                     /translation="MASPTPCYHCGLPVPAGSRFQAQVLGATREMCCPGCQAVAEAIV
                     SGGLEHYYKHRSESAANPQSLPQALPDELAMYDRSDVQQPFVEHDGELSETCLLIEGI
                     SCAACGWLIEKHLRTLPGVSEAHLNLSNHRLQVRWADSQLPLSQLLKALRKIGYAAHP
                     WQADAAAEQLQRENRRAMRELGVAGMLWMQVMMATMATWPEFNIDLSPELDKILRWVS
                     LFLTTPIVFYCCGQFFRGALRDLRTRHLTMDVSVSLAIGGAYIAGIWSTVTGQGELYF
                     DAVGMFALFLLAGRYLERRARERTAAATAQLVNLLPASCLRLAADGQSQRILLSELRV
                     GDQVLVPPGALLPADGRIVQGQSSVDESLLTGEYLPQPRNVGDSVTAGTLNVEGPLQI
                     EVQALGDATRLSAIVRLLERAQSDKPRLAVLADQVAQWFLLIVLMVAAIVGLVWWQLD
                     PSRAFWIVLSLLVATCPCALALATPTALTAATGSLHKLGMLLTRGHVLEGLNQIDTLV
                     LDKTGTLTEGRLTLSAIHPLRELDADACLALAAALENRSEHPIARAFGQAREAAEAVD
                     SHPGLGLQGRVGNRVLRIGEAGFVCALSAQPAPAIAGEHGQWLLLGDEQGPLAWFVLN
                     DRLRDDAPQLIDMAKGKGWQIMLLSGDSSPMVGEVARQLGIDDARGGLTPDAKLDVLK
                     QLHSQGRRVLMLGDGVNDVPVLAAADISVAMGSATDLAKTSADAVLLSNRLSSLVQAL
                     HMAQRTRRIIIENLTWASLYNGLVLPFAAIGWITPIWAALGMSVSSLLVVVNALRLTR
                     LPKP"
     gene            complement(10035..10559)
                     /locus_tag="VFF22_18250"
     CDS             complement(10035..10559)
                     /locus_tag="VFF22_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HZX18798.1"
                     /translation="MSEPIAEKNAEIIKPWYKQFWPWFLIGLLAYSVVQGLTLLTIAT
                     KNPPGLISDDYYDVGKGINQSLERENHAIRLKLHASLLLNDDNGTATLQLSGNSRPQQ
                     LVLNLISPTQPERDRRVILQPQADGSYSGQMVDAVKGRRFVELIGQEGGKDWRLFEEE
                     NVQSGQNILLGDAQ"
     gene            complement(10572..11978)
                     /gene="ccoG"
                     /locus_tag="VFF22_18255"
     CDS             complement(10572..11978)
                     /gene="ccoG"
                     /locus_tag="VFF22_18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HZX18799.1"
                     /translation="MSAQIPVKDVTPAKVEVVDLYANREKIYTRSFTGIFRNLRMLGG
                     AALFLLYFGTVWLTWNGRQAVWWNLPERKFYIFGSTYWPQDFVLLSALLIIAAFGLFF
                     ITVFAGRVWCGYTCPQSVFTWVFMWAEKVTEGDRNQRMKLDKAPMTTQKFLRKLAKHG
                     IWVGVSLVTAITFVGYFTPIRELIPELFTLQAGGWALFWVGFFTLATYGNAGYLREQV
                     CIYMCPYARFQSVMFDQDTLIVSYDPRRGESRGPRKRDADYKAEGLGDCIDCKMCVHV
                     CPTGIDIRDGLQIECIGCAACIDACDSIMDKMNYPKGLISYTTEHNLSGQKTHLVRPR
                     LIGYAVALTAMFCVFAWAIADRSLVELDVLKDRVLYRENEEGRIENVYTLKIMNKAQH
                     EVTYLIEADGLDGLVYEGKREIKAIAGEVLSLPVELSIDPEKLPSSTNEITFRLHSAD
                     DPSIKTDAASRFIGPSVR"
     gene            complement(12120..13109)
                     /gene="ccoP"
                     /locus_tag="VFF22_18260"
     CDS             complement(12120..13109)
                     /gene="ccoP"
                     /locus_tag="VFF22_18260"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009396217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HZX18800.1"
                     /translation="MTTFWSWYVTILSLGTIFALTWLIFGTRKGQREETTEETVGHSF
                     DGIEEFDNPLPKWWFMLFVGTIIFALGYLALYPGLGNWKGLLPGYDYLDTKAKTPFAT
                     NIEIAGGVERNAGWTGVHQWEKEMAKAEGQYGPIFAKYAAMPIEQVAQDEQALKMGGR
                     LFASNCSVCHGSDAKGAYGFPNLTDNDWLHGGEPETIKTTIMAGRQGVMPAQLPVIGE
                     EGIRNVAGYIRTLSGLKNPEGIEVDLAAGQDIFATTCAVCHGAEGKGQQAMGAPNLTD
                     KTWLYGSSFAQVQQTLRYGRNGKMPAQADFLGNDKVHLLAAYVYSLSQAKIEK"
BASE COUNT         2544 a         3750 c         4264 g         2609 t
ORIGIN      
        1 ctcacaagcc aacttttagg tagggttcgt catgcaggca gacgccagcg agcagcagcc
       61 agccgcagaa aaagccactg aggcccccgg caagaccctg ttactggagg ccgcgttacg
      121 tctcacctca accagtcgca gtctgagtaa tctcggcctg cgcgagctgg cccgtgaggc
      181 ggggttgaac cccaatacct tctaccggca cttcaaggat gtggatgacc tgggcctgac
      241 cgtgatccgg caaatctcca cccaactgcg ccaaccgcta cgcgatctgc gccgtgaagc
      301 cgccgaacgg gcggtgcagt cgactcaggc aagctcgccg atgctgtttg gcatcgacct
      361 gcaacgcggg cggcgggtgt gtcatgaaac cgtgcagctg ttttttgatt ttgttgcgca
      421 aaatcctgaa gccttcatcc tcggcgtgcg cgaactgcat ggcgcctctg ccgtgctgcg
      481 cgcggcgctg caggaggtga tggatgagtt tggcgcggac atggccgagg acatcattgc
      541 cttcaagctg ctgccggaaa tggtcaatcc ggcggtggtg ctgcaggtgt cgaacctgat
      601 cagtcgcaac ctgttccaac tgtcgctgga ctatatcggt caggccgaac ggcaagcggc
      661 aatctgtgcg ctggctgaag agcagatcgt tatgctgttt accggggcca gcgtgttgca
      721 gggcctgggg cagctaaagt tgcctgtgag ttgacccttt agtggagatc agcagcatgg
      781 ttgcattacc cctgttcggc ggttgcgcat gtggcaatgt gcgttaccgc ctggaaagcc
      841 tgccgggcga cagtggctac tgtcactgcc gtacctgcca gaaaatcggt gctacaccgg
      901 ccttgccctg ggcaatggtg ccggcgacgg cgctgcatta cttgagtggt gatattgggg
      961 tttttcactc cagtgctcgc ggggagcggc gcttctgtcg tgattgcggc actccgctgg
     1021 agtaccgcga gcagggaggt gaagaggttg ggctcaatag cgtgacgctg gatcaccctg
     1081 agtggtttcc accgcaaaag cacatctggt acgcatcgaa cctgccctgg tttgaactgg
     1141 cggacacgtt gccgcggttt gccagggaaa gctgaaccgg gggctgcccc ccccccggca
     1201 actttgtgta ttagctgtta aagcccagac gctcgcgcca gcgctgctct agcgccttgc
     1261 tgtcgtggtc cagagggtgg aagccggggc ggtagtaatc caggtacggc aggagcagtt
     1321 tggtcaggaa gccgttgcgt gggccaaaca gagttttcag gcccttgccc cagctgcgcc
     1381 agttgaacag ctggccgtct ttgcgcagca ggtgcacttg gaaccagccg atcacgccga
     1441 ggaagatcac ggtggtgatg gccatcatga tggtgcgggt gaagtagccg ccatagacct
     1501 gttgatacac gtcgtagcac accgccttgt gctcgttttc ttcgatggcg tgccacatcc
     1561 acagctgata cagcttggga tcgttcatct ggctggtcag gtcttcgcgc tgtagcagct
     1621 gctcagccat ggtcgcggtg aaatgttcca gggcgcaggt ggcggccagg cgatgtttct
     1681 tggtgctgaa cttggtgacc cattccagca gcaccttgat gcgcagctcg aggatttcca
     1741 ggtcgatatt gtgctcggcg gcgtagtcgt tataggccgc gtgctctttg gagtgcatgg
     1801 cctcctggcc gataaaggcg ctgatgtcct tcttcagttg tggctcggtc acctgatcac
     1861 gcacggcgcg tacgctgtcg acgaagaatt tctcgccata gggaaacagc gaagacaggt
     1921 tgttcataaa gtggctcatg aactggtcgc cagcaaacca gaacttctgg ctttcatcga
     1981 aactgaagtc cattcgacgc acgggaaagc tggcggttag ggtgctggtt ttggctgtca
     2041 tataaaggcg actcctcggc atattggtcg catcgcgacc gctcttgtta tggggcttgg
     2101 gacgacagac agagctgtct tgggccgtga tttatcttta gccgagggcg ttttaggcgc
     2161 gaggtcattt ctggccagtg cagccggaat tgggccaact tatggatagt tgtcggacag
     2221 ttcgtcaggc ccgtgcgctt taggggcgcg agctagcagg gagagggggg cgtggcggga
     2281 atgcgatcta tgcctgtact gcatcgcgcc agggcggtgc gatgcagtac tgtacttgcg
     2341 gcttggcagg ggttggcctc agcgctgctg gcgcagctcg gcgacttccg cacgcagggc
     2401 gcgcagttca tcgagcagca attgctcggt gggcgaaggc ggtgccagtt tggcttcctg
     2461 ctcatgggtg gtctgcatgg cattaaccac cacggcgata aacaggttaa gcatcataaa
     2521 ggtggcaatc aggataaacg gcaggaaata catccacgcc agcgggaact gctccatcac
     2581 cgggcgcacg atacccatgg accagctttc cagcgtcatc acctgaaaca gggtgtagag
     2641 gctggcaccg aggctaccga accactccgg aaaggctgcg ccaaacagtt gagtggccat
     2701 taccgctgct acgtagaaca ccaggccgag cagcatggcg atgctgccca tgcccggcag
     2761 cgaggacagc agcgcttcga ccacccggcg catgttcggg ttgatcgaaa tcagccgcag
     2821 cacgcgcaac acgcgcagcg cacgcagcac ggcgaatggc ccgctggacg gcaccagcgc
     2881 cacgcccacc accaggcagt cgaataccgc ccagggatca cgcagcaggc ccaggccacg
     2941 ggcgacaaag cgcagcgcca gctccaggat aaataccgcg agaatcagtt gatccagcgc
     3001 caatagtagc tcgccccagt cggccatcag cccagggtag gtctggatgc cgagaattgc
     3061 ggcgttgagc acgatcagag tggtgaccag gcgggtgatg attttgcttt ccatcagcgc
     3121 accgaggcgc agacgccagt ggctggacgt cgggttcatt gatatttcat tcatggcggg
     3181 tggggtactt gtgtaaacgg tggcgcgagt cagttcgcga gggtcagcct ggccccgaca
     3241 tgcagcgctg ccgtcagggc cgtggagcag atttattggt cggtgagggc gaagctggtc
     3301 agtgcgaagg tggggatgcc gctgtcctgc agtttttgcg agccgcccag ttcgggcagg
     3361 tcaatgatcg cggcggcttc gatgatgctc gcgcccatgc gtcgtactag ttgcgcggcg
     3421 gccagcaggg tgccaccggt ggcgatcagg tcatcgaaaa tcagcacttt atcgccttcg
     3481 cagaggctgt cggcgtgcac ttccagtttg gcttcgccgt actcggtctg gtaactctcg
     3541 ctgagtacat cggcgggcag tttgccctgc ttgcgaaaga ggatcagtgg tttgttcagt
     3601 tcataggcga tgatcgagcc gatcagaaaa ccgcgcgcat ccatggcgcc gatatggctg
     3661 aaatccgctt cgacgtaacg ctggataaaa ctgtcgacga ccatgcgcag ggcgcgcggc
     3721 gagtgaaaca gcggggtgat atcgcgaaag atcacccccg gcttggggaa gtccaccacc
     3781 gggcggatca gggttttgat agtgaattcg tcgaagatca tcggctaggt cctgcgctgc
     3841 gcgggtcgat cagccctcga ggttaccgcc ggccagggcg cacagctcaa tgggatcgag
     3901 gatatgcact tccttacctt cggcttcgag cagcttactc tgttggaagc gggtaaatac
     3961 ccgcgaaacg gtctccaccg caaggcccaa atagttacca atttcgttgc gcgacatggc
     4021 caggcggaac tgattggccg agaagccgcg ggcgcggaag cgtgccgaga ggttgactag
     4081 gaaggtggcg atgcgttcat cggcggtttt tttcgagagc agcagcatca tctgctgatc
     4141 gtcgcggatc tccctgctca tgatgcgcat tagttggcgg cgcagttgtg gcagttgtat
     4201 cgacaattcg tcgaggcgct cgaaagggat ttcgcacacc gaggtggttt ccaatgccat
     4261 ggctgacacc gggtacgctt cactgtccat gccggacagg ccgaccagtt cgctgggcag
     4321 gtgaaagcca gtgatctgct cgtcgccgct gtcgctcagg ctgaaggttt tcagcgcacc
     4381 ggaacgtact gcgaataccg aattgaaggt gtcgccctgg cgaaagagga attcgccttt
     4441 tttcagcggg cggccgcgtt taacgatttc atcgagcgcg tccatgtctt ccaggttcaa
     4501 ggacagcggc aggcacaagc tcgccaggct gcaatccttg caatgggctt ggtgtggggt
     4561 atgcaacttg atgctttcgg acatcgctgg tgatcctggg aggtaaacac acaatagccg
     4621 taaggttaca gcagggcgca gggtagggcc actaagacgc aagtctgatc gaatgttcaa
     4681 gttttagccg gtacggccga ttactcaagc gactttaagt atctgcaagg agcattcgta
     4741 tgcgtctgga atcgactcaa ttgagcaccc gtatgaacct ggcgctggag agcaggagtg
     4801 ctgcgaccga aggcgttgcg gcgagccgcg aggaggagaa gcaggtactg cgcgacagcc
     4861 tgcgggtaag tctgtcggag ctgggtaagg cgcgctccgc tgccgcgcag aagaataagg
     4921 acatcgatga gagcagcctg ccggatgttc tcaaggatat tctcaagatg attcgcgagc
     4981 tcaaggcgca gatcgcggcg aagaaagccg agctggaaga ggtaatgagc gatcagagcc
     5041 tagaccccga ggccaaacgc ataaagatcg aaatgctacg gggcgagctg gcctcgctgc
     5101 agggcgcgtt gagcagcgcc aacgcaacgc tgatcaaggt catgcgtgag cagaacctga
     5161 gtgatcagca gatgcaggaa gtgtccagct tgcttatgaa ataaacgtgc tagatcaccc
     5221 gggaaaagcg ttgttgattc agctcgctga ggtagcgatc aaacagcatg cacagcgagc
     5281 ggaccagcag gcgccccgct gggcgtacct cgatgccctg ggctgttagc tcaatcagcc
     5341 catcactggc catctgctgc agttgcggcc atacctcggc aaagtagctg cgaaaatcga
     5401 tggcataggc ctgttcgata tcggcaaaac gcagctggaa atgacagatc agctgctgaa
     5461 ttactgcacg ccgtagacgg tcgtcggcat cgcagtgcag gccgcgcaaa gtggccagtt
     5521 cgctgtggcc taggctgtcc tggtatttgc ttaagtcgct gttgttttgg cagtagaggt
     5581 caccaatctg gctgattgat gacactccca ggcctatcag gtcgcaatga ccgtgggtgg
     5641 tgtagccctg gaagttgcgc tgcaggctgc cgtcttcctg agcgctggcc agttcgtcgt
     5701 cgggcagggc gaagtgatcc atgccgatat agcggtaacc ggcggcgctc agttgctcga
     5761 tgctggcctg cagcatggcc agcttgtcgc ctggattggg cagatcgctg gtgttgatcc
     5821 gccgctgcgg cataaagcgc tccggcaggt gggcgtagtt gaacagcgac agacggtcgg
     5881 gttgcagggc aatcacctca gccacggtgc gggcgaagcg ctccggggtt tgcaacggca
     5941 ggccgtagat caggtcgatg tttaccgagc ggaactgcag ggtgcgcgcg gcctcgatga
     6001 tggcgcgggt ttcttccagg gtctgcaatc ggttgaccgc ccgctgcacg accggatcga
     6061 ggtcctgcac gcccaggctg acgcggttga agcccagctc gcggagcagg cccatggtcg
     6121 accagtcggc ttcgcgcggg tcgatctcga tactgtagtc gccgctgtcg tcatccagca
     6181 gattgaaatg cttgcgcagg tggcccatca ggtggcgcag ctcatcgtgg ctgagaaagg
     6241 tcggcgtgcc gccgccgaag tgcagctgct cgaccttttg ctgtgggtcg agatggcggc
     6301 cgatgatctc gatttcccgc tgcaattttt ccagataggg cagggcgcgg ccgcgatcct
     6361 tggtaatgac cttgttgcag gcgcagtagt agcagatgtg tgcgcagaac gggatgtgca
     6421 catacaacga caacggccgc agggctttgc ggctctcgcg cagggcgtgc agcaggtcga
     6481 actggctgac ccgctcatgg aactgcacgg cagtcgggta cgacgtgtag cgtgggccgg
     6541 cttggtcgta gcggcggatt agtgagctgt cccactggat ggcgttgagc atgcgggtct
     6601 tcccggtttg gctttcaatg ccgggaagtc taagagcgct gcgccagggg tgttttgact
     6661 tgtatcaagc ggccgctgat cgcttagtgg cccatcagcc agtgctggtg cgggccgggc
     6721 agggtccata ggccgaagag gatcaccaga atgccgccgg ccatgcgcac gccttgcttg
     6781 cgcagtaggc cggtcaggcg ttcggcagcc atgccggtgg ccagtagtac tggcagggtg
     6841 ccgaggccga aggccagcat cagcgccgcg ctgtcgactg cattcccctg gctggcggcc
     6901 cacagcaggg tgctgtagac caggccgcag ggcagccagc cccacaggct gccgagcacc
     6961 aaggcgcgcg gcaggctggt gactggcata aagcgccggg tcagcggttg tatataccgc
     7021 cacaggccac gaccgagggc ttcgatacgg gtcaggccgc tccaccaacc ggccaggtac
     7081 aggcccatgg cgatcagcag cagcgctgca accacgcgca ggcccatgac caacgggcta
     7141 ttggctactg cccagccggc caagccaagc agcaggccgg ctacgctata gctgaagatg
     7201 cgccctaggt tataggccag cagtaactta aagcgttgtg cgcgttgctc gggtgggatg
     7261 gccagggtca gtgcacccat caggccgccg cacatgccca ggcagtggcc gccgccgagc
     7321 aggccgagaa tcagtgccga aaccagtagc ggcagtagct caagcaccgg gcttgtcccc
     7381 tgattccttg gactgttcct cttcttgtac ctgactgact gcagcctggt gcttagggtc
     7441 ttcgtcgtcg aacaggatgc tgtgggccgg gccgtcgagg tcgtcgtact gaccgctgtc
     7501 caccgcccag aagaacagcc agatagcaaa acccaccagg gcgatggcga ccgggatcag
     7561 gatatagagg gcgggcatgg gatctccggg cagcgatagg gttgggcgac gctcacctta
     7621 cggcttgggc aggcgcgtca ggcgcagagc gttaaccacc accagcaatg agctgactga
     7681 catacccagc gcggcccaga tgggggtgat ccaaccgatg gcggcgaagg gcaggaccag
     7741 gccattgtac aggctggccc aagtcaggtt ctcgatgatg atgcgccgcg ttcgttgcgc
     7801 catgtgtagc gcctgcacca ggctgctcag gcggttggac agcagcacgg catcggcgct
     7861 ggtttttgcc aggtcggtgg cgctacccat ggccacgctg atatcggcgg cggccaagac
     7921 cggcacatcg ttgaccccgt caccgagcat cagcactcgg cggccctggc tgtgcagttg
     7981 tttcagtacg tccagcttgg catccggggt gaggccgcca cgggcgtcgt cgatacccag
     8041 ctggcgcgcc acttcaccga ccatgggcga gctgtcgccg gacagcagca tgatctgcca
     8101 acctttgccc ttggccatgt cgatcagttg tggtgcatcg tcgcgcaagc ggtcattcaa
     8161 gacgaaccag gccagcggcc cttgctcatc gcccagcagc agccattggc cgtgctcacc
     8221 ggcaatcgcc ggcgcgggtt gcgcactcag cgcgcaaaca aaacccgcct caccgatgcg
     8281 cagtacccgg ttgccgacgc gcccctgtag gcccaggccc ggatgactgt cgaccgcttc
     8341 tgcggcctcg cgagcctggc cgaaggcgcg ggcaatcggg tgttcggaac ggttttccag
     8401 ggcggcggcc agggccagac aggcgtcggc atccagctca cgcaagggat gaatggcgct
     8461 aagggtcagg cggccttcag tgagggtgcc ggtcttgtcc agcaccaggg tgtcgatctg
     8521 gttcaggcct tccagcacat ggccacgggt caacagcatg cctagcttgt gcagcgaacc
     8581 ggttgctgcg gtcagtgccg tgggtgtagc cagcgccagg gcgcaagggc aggtggcgac
     8641 cagtagggaa agaacgatcc aaaacgcgcg agatgggtcg agctgccacc agaccaagcc
     8701 gacgatggcc gccaccatca gcacgatcag caggaaccac tgcgcgacct gatcggccaa
     8761 tacggccagg cgcggcttgt cgctctgcgc acgctccagt aggcggacaa tggccgatag
     8821 ccgcgtggca tcgccgaggg cctgcacttc aatctgtagc ggcccttcga cattgagtgt
     8881 gccggcggta acgctgtcgc cgacattacg cggttgcggc aggtattcgc cggtgagcag
     8941 cgactcatcg acgctcgatt ggccctgaac aatccgccca tcagccggca acagggcgcc
     9001 gggcggtacc agcacctgat cgccgacacg cagctcgcta agcagaatgc gctggctctg
     9061 gccgtcagca gccaggcgta ggcaggaggc cggcagcaga ttgaccagct gcgcggtggc
     9121 ggctgccgtg cgctctcggg cgcggcgttc caggtagcga ccggccagca gaaatagagc
     9181 aaacatgccc accgcatcga aatacagctc accctggccg gtgacggttg accagatacc
     9241 ggcgatgtag gcaccgccga tggccaggga caccgacaca tccatggtca ggtgtcgggt
     9301 gcgcaagtcg cgcagcgcgc cacggaagaa ctggccgcag cagtagaaga caatcggtgt
     9361 ggtaagaaac aggctgaccc agcgcaggat cttgtccagc tcggggctta ggtcgatatt
     9421 gaattccggc caggtggcca tggtcgccat catcacctgc atccacagca tgccggctac
     9481 accgagctca cgcatggcgc ggcgattctc gcgttgcagc tgttctgcgg cggcatcggc
     9541 ctgccaggga tgggcggcgt agccgatctt gcgcagcgcc ttgagcagtt ggctcaacgg
     9601 caactggcta tcagcccagc gcacctgcag gcggtggttg gacaggttga ggtgcgcctc
     9661 actaacgccg ggcaaggtgc gcaggtgttt ctcgatcagc cagccacagg ccgcgcagct
     9721 gatgccttcg atcagcaggc aggtttcgct gagttcgccg tcatgttcga caaagggctg
     9781 ctgcacatcg ctacggtcat acatcgccag ctcatcgggc agcgcctggg gcaggctctg
     9841 tgggttggcg gcgctttcgc tgcggtgttt gtagtagtgc tccaggccgc cgctgacgat
     9901 ggcttcggcc acggcctgac agcccgggca gcacatctcg cgggttgcgc ccagtacctg
     9961 agcctggaaa cggctgcctg cgggtaccgg caggccacag tggtagcaag gagtcgggct
    10021 ggccatcggt gcgcttattg agcatccccg aggaggatgt tctgcccgct ttggacgttt
    10081 tcttcttcga acaggcgcca gtccttgccg ccttcttggc cgatcagctc gacaaagcgc
    10141 cgacctttca cggcatcgac catctgcccg ctgtagctgc catcggcctg cggctgcagg
    10201 atcacccggc ggtcgcgctc aggctgggtc ggcgagatca ggttaagcac cagctgctgc
    10261 ggacgactgt tgccgctcag ctgcagggtg gcggtgccgt tgtcatcgtt gagcagcagg
    10321 ctggcgtgca gcttgaggcg gatggcgtgg ttttcccgct ccagggactg gttgataccc
    10381 ttgccgacat cgtaatagtc atcggaaatc agccccggcg gattcttcgt ggcgatggtc
    10441 agcagggtca gcccctggac cactgagtag gccagcaggc ctatcaggaa ccagggccag
    10501 aattgcttgt accagggctt gatgatctcg gcgttctttt cagctatggg ctcagacatg
    10561 gtcttctcta atcagcgcac gctggggccg ataaagcggc tggcggcgtc ggttttaatg
    10621 cttgggtcat ccgctgagtg caggcggaag gtgatttcat tggtgctcga aggcagcttt
    10681 tctgggtcga tggacagctc gactggcaat gacagcacct cgccagcgat tgccttgatc
    10741 tcgcgcttgc cctcgtacac caggccgtcg aggccgtcgg cttcgatcag gtaagtaacc
    10801 tcgtgctggg ccttgttcat gatcttcagg gtgtagacgt tctcgatccg gccttcttcg
    10861 ttctcgcggt agagcacgcg gtctttcagt acgtccaatt ccaccagcga acggtcggcg
    10921 atggcccagg cgaagacgca gaacatggcg gtcagggcca ccgcataacc gatcaggcgc
    10981 gggcgcacca ggtgggtctt ctgtccggag aggttgtgct cggtggtgta gctgatcagc
    11041 cccttggggt agttcatctt gtccatgatg ctgtcgcagg catcgatgca ggcggcgcaa
    11101 ccgatgcact cgatctgtag gccgtcgcgg atgtcgatgc cggtagggca gacgtgcacg
    11161 cacatcttgc agtcgatgca gtcgcccagg ccttcggctt tgtaatcggc atcgcgcttg
    11221 cgcgggccac ggctctcacc gcgacgcggg tcgtaggaga cgatcagggt gtcttggtcg
    11281 aacatcacgc tctggaagcg cgcatagggg cacatgtaga tgcacacttg ctcacgcagg
    11341 tagccggcgt tgccgtaggt ggcgagggta aagaagccga cccagaacag cgcccagccg
    11401 cccgcttgca gggtgaacag ctcggggatt agttcgcgaa tcggcgtgaa gtagccgacg
    11461 aaagtgatcg cggtgaccag cgacacgccg acccagatgc cgtgcttggc cagcttgcgc
    11521 agaaatttct gcgtggtcat cggtgccttg tcgagcttca tgcgctggtt gcgatcacct
    11581 tcggtgacct tttccgccca catgaatacc caggtgaata cgctttgcgg gcaggtgtag
    11641 ccgcaccaca cgcggccagc aaacacggtg ataaagaaca ggccgaaggc ggcgatgatc
    11701 aacagcgccg agagcaggac gaaatcctgc ggccagtagg tggagccgaa aatatagaac
    11761 ttgcgctccg gcaggttcca ccagacggcc tgacggccat tccaggtcaa ccatacggtg
    11821 ccgaagtaca acaggaagag ggctgcgcca ccgagcatgc gcaagttgcg gaagatgccg
    11881 gtgaaggagc gggtgtagat cttttcgcgg ttggcgtaca ggtcaacaac ttcgaccttg
    11941 gcaggggtga cgtctttaac tggaatctgt gcactcatca gttcgtacca cggcggttga
    12001 ttggctgccc tggtcgatac gtgccggccg gggtcatacg ggctcttgcg ctatggtacg
    12061 cctgacaatt gcctgacagg tgcgacctgc ggtcgcgcct gtcaggccag gctggagaat
    12121 cacttctcga tttttgcttg cgacaggctg tacacgtaag ccgccagcag atgcacctta
    12181 tcgttgccca ggaagtcagc ctgggctggc atcttgccgt tacggccata gcgcagagtt
    12241 tgttgtacct gagcaaaact ggagccgtag agccaggttt tgtcggtcag gttgggtgcg
    12301 cccatggcct gttggccctt accttcagcg ccatgacaga ctgcacaagt agtcgcgaag
    12361 atgtcttgac ctgcggcaag gtcaacctcg ataccttccg ggtttttcaa accggagagt
    12421 gtgcggatat agccagccac attgcggata ccttcctcac cgatcaccgg cagctgcgca
    12481 ggcattacgc cttggcggcc agccataatg gtggtcttga tggtttccgg ctcgccgcca
    12541 tgcagccaat cgttgtcggt caggttcggg aagccatatg cgcctttggc gtcggagccg
    12601 tggcagaccg agcagttgga agcaaacagg cgaccgccca ttttcagcgc ttgctcgtcc
    12661 tgggccactt gctcgatcgg catggcagca tatttggcga agatcgggcc gtattggcct
    12721 tcagctttag ccatttcctt ttcccactga tgcacgccgg tccagcccgc attgcgctct
    12781 acaccaccag cgatttcgat attggtggcg aagggcgttt tggccttggt gtcgaggtag
    12841 tcgtagcctg gcagcagacc tttccagtta cccaggcccg ggtagagcgc caggtagccg
    12901 agggcaaaga tgatggtgcc gacaaatagc atgaaccacc acttgggcag cgggttgtcg
    12961 aactcttcga taccgtcgaa ggaatggccg acggtttctt cggtggtttc ttcgcgctga
    13021 cccttacggg tgccgaagat cagccaagtc agggcaaaga tggtgcccag agacagaatg
    13081 gttacgtacc aactccagaa cgtggtcatt ggttattgct cctggaagat tgctcgtcac
    13141 gctcgttggg tttgggatcg tcggcga
//
LOCUS       DASRUM010000272         8944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2306562, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000272 DASRUM010000000
VERSION     DASRUM010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8944
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2306562"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1981
                     /locus_tag="VFF22_18265"
     CDS             116..1981
                     /locus_tag="VFF22_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020296096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZX18801.1"
                     /translation="MTHALRALFIHVSGIALLSVASLALAGPKHAQTLYDEPPKYPAN
                     FKHFDYVNPDAPKGGTLRQAGFGGFDSLNPFINKGVPADDIGMIYDTLTRHGLDEPFT
                     EYGLIAEKIEKAPDNAWVRFYLRPEARFHDGQPVTAEDVKFTFETLMSKGAPMYRGYY
                     ADVDKVEIESPHRVRFVFKHAGNRELPLIVGQLPVLPKHWWAERDFSKGNLDVPLGSG
                     PYKVADVQAGRSIRYERVKDWWGKDLPVNRGFYNFDTLEIDYYRDNTVALEALKAGQF
                     DYWLEISAKNWATAYNSPAVANGQLIKEEIANHNPTGMQGFIFNTRRPLFADRRVREA
                     LGLLFDFEWANRQLFNGAYTRTRSYFDNSELASVGLPSADELKLLEPLRAQIPPQVFT
                     DEYQPSVTDGSGIIREQQRRAYQLLQEAGWRVDGDKMLDSTGKPVSFEFLLAQTEFER
                     ILLPFKRNLADLGIDLVIRRVDVSQYINRLRSRDFDLIVGGFGQSNSPGNEQREYWHS
                     SSADNPGSRNFIGLKDPAVDKLVEGLINADSRKSLITHTRALDRVLLWGQYVVPNWHI
                     KTWRVAYWNRFEHPKVTPQYDIGLHTWWARDFPDEAAPEAAADEAAAHPATDVEQ"
     gene            1983..3056
                     /locus_tag="VFF22_18270"
     CDS             1983..3056
                     /locus_tag="VFF22_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HZX18802.1"
                     /translation="MLAYIFRRLLLIIPTLFGILLINFIIIQAAPGGPVEQMIAKLEG
                     FDGATSRIAGGGAEVSVAGSNYRGAQGLDPELVAEIERMYGFDKSAPERFWLMLKSYA
                     QLDFGSSFFRDAEVIDLIIEKMPVSISLGLWSTLIMYLVSIPLGIAKATRHGSAFDVW
                     TSSMIIVGYAIPAFLFAILLIVLFAGGSYWDWFPLRGLTSNNFDELSFTGKLLDYFWH
                     LALPVTALVIGNFATLTLLTKNSFLDEINKQYVVTARAKGLSNNRVLYGHVFRNAMLI
                     IIAGFPAAFIGIFFTGSLLIEVIFSLDGLGLMSFEAAINRDYPVVFGTLFIFTLLGLV
                     VKLIGDITYTLVDPRIDFESREG"
     gene            3058..4077
                     /locus_tag="VFF22_18275"
     CDS             3058..4077
                     /locus_tag="VFF22_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX18803.1"
                     /translation="MKLSPINQRRFQLFKAHKRGWWSLWIFLALFFVTLGAELIANDK
                     PLVVSYDSELYFPVFKRYPETTFGGEFPLQANYKSPYIQELIKEKDGWMIWPPIRYSY
                     SSINYDLQVPAPAPPSAENLLGTDDQGRDVLARVIYGFRISVLFALILTLASSVIGVF
                     AGALQGFYGGWVDLVGQRFLEIWSGLPVLYLLIILASFVQPNFWWLLGIMLLFSWMSL
                     VDVVRAEFLRGRNLEYVRAARALGMQNGSIMFRHILPNAMISTMTFMPFILTGAIGTL
                     TALDFLGFGLPPGAPSLGELVAQGKSNLQAPWLGISAFAVLALMLSLLVFIGEAARDA
                     FDPRK"
     gene            4079..5686
                     /locus_tag="VFF22_18280"
     CDS             4079..5686
                     /locus_tag="VFF22_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX18804.1"
                     /translation="MTDSNTLIEVRDLAVEFVTGSLKQRVLEGVSFDIKRGETLALVG
                     ESGSGKSVTAHSILRLLPYPLAQHPSGSIHYAGQDLLKLNENKLRGIRGNRIAMVFQE
                     PMTSLNPLHSIEKQINEVLALHKGLRGKAATARTLELLELVGIPEPHKRLKTYPHELS
                     GGQRQRVVIAMALANEPELLIADEPTTALDVTVQLKILELLKDLQARLGMAILLISHD
                     LNLVRRIAHRVCVMQRGHIVEQAACETLFKDPQHPYTRELLGAEPTGGPVDQPPGKTM
                     LEIDNLRVWFPIKKGLLRRTVDHVKAVDGIDFSLPQGHTLGIVGESGSGKSTLGLAIL
                     RLLGSQGGIRFQGKAIDSLSQEDVRPLRRQMQVVFQDPFGSLSPRMCVGQIVGEGLQI
                     HRMGSEAEQEQAIIAALVEVGLDPETRHRYPHEFSGGQRQRIAIARALVLKPALILLD
                     EPTSALDRTVQRQVVELLRSLQAKYNLTYLFISHDLAVVRALSHQVMVVKQGKVVEQG
                     PAARVFAAPQDIYTQQLLEAAFMVPAD"
     gene            5718..6512
                     /gene="fabI"
                     /locus_tag="VFF22_18285"
     CDS             5718..6512
                     /gene="fabI"
                     /locus_tag="VFF22_18285"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004660733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HZX18805.1"
                     /translation="MGFLSGKRVLIVGVASKLSIASGIAAAMHREGAELAFTYQNEKL
                     KGRVEGFAADWGSGPELCFPCDVANDEEIASVFEALSKKWDGLDCIVHSVGFAPGDQL
                     DGDFTDVTTREGFRIAHDISAYSFVALAKAGREMMKGRNGSLLTLSYLGAERTMPNYN
                     VMGMAKASLEAGVRYLAGSLGPEGTRVNAISAGPIRTLAASGIKSFRKMLAANEKQTP
                     LRRNVTIDEVGNAGAFLCSDLASGVSGEIMYVDGGFNTTAMGAMEE"
     gene            complement(6582..8459)
                     /locus_tag="VFF22_18290"
     CDS             complement(6582..8459)
                     /locus_tag="VFF22_18290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014339301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HZX18806.1"
                     /translation="MLQNIRDNSQGWIAKTIIGIIVALLALTGVDAIFTSTSNSQNAA
                     EVNGEKITSAELSQAVEMQRRQLLQQLGRDFDASLLDEKLLREAALNGLVERTLLLDS
                     AKDAKFAFSQQALDQLILKTPEFLVDGQFNAERFDQVIRQLGYSRLQFRQMLEQEMLI
                     GQLRAGLANSGFVTDAQVQAFAALEKQTRDFATLTLKADLDAVTLSDDDIKAYYDAQA
                     SEFMSPEQVVLEYVELKKDAFFDQVEVTDEELQSAYQSEIANLAEQRRAAHILVEVND
                     KLNDEQAQAKLVAVQKRLEQGEDFAALAKEVSDDSGSAGEGGDLGFAGPGVYDPAFEE
                     ALYALDENAVSAPVRSEFGWHLIKLLGVQAPEVPSFASLEEKLQRDLKARQVEQRFVE
                     VSKDLEDAAFEASDLAQPAQELGLQVKTTEAFGRQGGTEGLTANRQVIQAAFSDEVLE
                     DGSNSSVIELDPNTVVVVRVKEHNKPEQLPLEQVADSIRAQLTKVRASEAVKAKGEEQ
                     LAALRDGQTPVTQADAAQGWNVVEAATRSQEGVEPVVLQALFRMPKPEAADKPTFAGL
                     SLSNGDFMIIRLNGVSQPQQTLSDEDKAMYSRFLASRVGQQDFEAFRQQLEETADIER
                     F"
     gene            complement(8680..8944)
                     /locus_tag="VFF22_18295"
                     /pseudo
     CDS             complement(8680..8944)
                     /locus_tag="VFF22_18295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="HU family DNA-binding protein"
BASE COUNT         1803 a         2850 c         2509 g         1782 t
ORIGIN      
        1 actggtatat cgattaccac cgcctggctt accgcaaccg atttgccttc gtcaccacgc
       61 cgccctacac cctgggcctg cgtacctggt ggctgaaatc catggagaac gctgaatgac
      121 ccatgcattg cgcgccctgt ttatccacgt cagcggcatt gccctgctta gcgttgccag
      181 cctggccctg gccggcccca agcacgcaca aaccctgtat gacgagccgc ccaagtaccc
      241 ggccaatttc aagcacttcg actacgtcaa cccggatgcg cccaaaggcg gcaccctgcg
      301 ccaagccggt ttcggcggct tcgatagcct caatcccttt atcaacaagg gcgtgccggc
      361 cgacgatatc ggcatgatct acgacaccct gacccgccac ggtctggatg aaccctttac
      421 cgaatacggc ctgatcgccg aaaagatcga gaaagccccg gacaatgcct gggtacgctt
      481 ctacctgcgc cccgaagccc gtttccacga cggccagcca gtgaccgccg aagacgtcaa
      541 attcaccttt gaaaccctga tgagcaaagg cgcgccgatg taccgcggct actacgccga
      601 tgtcgacaag gtagagatcg aaagccccca ccgcgtgcgt tttgtcttca agcatgccgg
      661 taaccgtgag ctaccgttga tcgtcggcca actgccggtg ctgccaaaac actggtgggc
      721 cgagcgtgat ttcagcaagg gcaacctcga cgtgccactg ggcagcgggc cctacaaggt
      781 ggcggacgtg caggccggcc gctcgatccg ctatgagcgg gtcaaggact ggtggggcaa
      841 ggacctgccg gtcaatcgcg gtttctataa cttcgacacc ctggaaattg actactaccg
      901 cgacaacacc gtcgccctgg aagcgctcaa ggctgggcag ttcgactact ggctggaaat
      961 cagcgcaaag aactgggcca ccgcctataa cagccctgca gttgccaacg gccagctgat
     1021 caaggaagag atcgccaacc acaaccccac cggcatgcag ggctttatct tcaacacccg
     1081 ccgcccgctg ttcgccgacc gccgcgtgcg tgaagctctg ggcctgctgt ttgatttcga
     1141 atgggccaat cggcagctat tcaacggcgc ctacacccgc acccgcagct acttcgacaa
     1201 ctccgaactg gcctccgtcg gcctgccctc agcagacgag ctgaagctgc tggagccatt
     1261 gcgcgcgcag attccgccgc aggtatttac cgacgagtac cagccctctg tcaccgatgg
     1321 cagcggcatc attcgtgagc agcaacgccg cgcttaccag ttgctgcaag aagccggctg
     1381 gcgcgtggat ggcgacaaga tgctcgacag cacgggcaag ccggtgagct tcgagtttct
     1441 gctggcgcaa accgaattcg aacgcatcct gctgccattc aaacgcaacc tggctgatct
     1501 gggcatcgac ctggtgatcc gccgtgtgga cgtgtcccag tacatcaacc gcctgcgttc
     1561 gcgcgacttt gacctgatcg tcggcggctt tggccagtcc aactcgcccg gcaacgagca
     1621 gcgcgaatac tggcactcca gcagcgccga caaccccggt agccgcaact ttatcggcct
     1681 gaaagacccg gcagtggaca agctggtcga gggcctgatc aacgccgact cgcgcaagag
     1741 cctgatcacc catactcgcg ccctcgaccg cgtgctgctg tggggccaat acgtggtgcc
     1801 caactggcat atcaaaacct ggcgcgtggc ctactggaac cgtttcgagc accccaaggt
     1861 caccccgcag tacgatatag gcctgcacac ctggtgggcc cgcgacttcc ccgatgaagc
     1921 agcaccagaa gcagcggccg acgaagcggc cgctcatccc gccacagacg tggagcaata
     1981 agatgctggc gtatatcttt cgtcggctac tgctgatcat ccccacgctg ttcggcattc
     2041 tgctgatcaa cttcatcatc atccaggccg cccccggtgg tccggtcgag cagatgatcg
     2101 ccaagctgga aggctttgac ggtgccacca gccggattgc cggcggcggc gccgaagtct
     2161 cggtggccgg ttctaactac cgcggcgccc agggtctgga cccggagctg gtggcggaaa
     2221 tcgagcgcat gtatggcttc gataaatccg ccccggaacg cttctggttg atgctcaaga
     2281 gctatgcgca gctggacttt ggctcgagct tcttccgcga tgccgaggtt atcgacctga
     2341 tcatcgagaa gatgccggtg tcgatctccc tggggctatg gagcacgctg atcatgtacc
     2401 tggtgtcgat tccgctgggc atcgccaagg ccacccgcca cggtagcgcc ttcgacgtgt
     2461 ggaccagctc gatgatcatc gtcggctacg ccatccccgc tttcctcttc gccatcctgc
     2521 tgatcgtgct gtttgccggc ggcagctact gggactggtt cccgctgcgc gggctgacct
     2581 caaacaactt cgacgaactg agctttaccg gcaagctgct cgactatttc tggcacctgg
     2641 cgctgcccgt cacggcgctg gtgatcggca acttcgccac cctgacgctg ctaaccaaaa
     2701 acagctttct cgatgagatc aacaagcagt acgtggtcac tgcccgcgcc aaggggctga
     2761 gtaacaaccg cgtgctctac ggccatgtgt tccgtaacgc catgctgatc atcatcgccg
     2821 gcttcccagc ggcctttatc ggcattttct ttaccggctc attgctgatc gaggtgatct
     2881 tctccctcga cggcctgggc ctgatgagtt tcgaggccgc catcaaccgc gactacccgg
     2941 tggtattcgg caccctgttt atcttcacgt tgctgggcct ggtggtgaaa ttgatcggcg
     3001 acattaccta caccctggtc gatccgcgta tcgacttcga aagccgggag ggttgagatg
     3061 aaactttccc caatcaatca acgccgcttc cagctgttca aggcgcacaa gcgcggctgg
     3121 tggtcgctgt ggatcttcct cgcgctgttc ttcgtcaccc tcggcgccga gctgatcgcc
     3181 aacgacaaac cgctggtggt gagctacgac agcgaactgt atttcccggt attcaagcgc
     3241 tacccggaaa ccaccttcgg cggcgaattc ccgctgcagg ccaactacaa gagcccgtat
     3301 atccaggagc tgataaagga aaaggacggc tggatgatct ggccgccgat ccgctacagc
     3361 tactccagca tcaattacga cctgcaagtg cccgccccgg cgccgccgtc ggcggaaaac
     3421 ctgctgggca ccgatgacca gggccgcgat gtattggcgc gggtgatcta tggcttccgc
     3481 atctccgtgc tgtttgccct gatcctgacc ctcgccagtt cggtgatcgg cgtgtttgcc
     3541 ggcgccctgc agggctttta tggcggctgg gtcgacttgg tcgggcagcg ttttctggaa
     3601 atctggtccg ggctgccggt gctctatctg ctgatcatcc tcgccagctt cgtgcagccg
     3661 aacttctggt ggctgctggg catcatgctg ctgttctcct ggatgagcct ggtggatgtg
     3721 gtgcgcgccg agttcctgcg tgggcgcaat ctggaatacg tgcgcgccgc ccgcgccctg
     3781 ggcatgcaga acggctcgat catgttccgc catatcctgc ccaacgcgat gatctccacc
     3841 atgaccttta tgccgtttat cctcactggc gcgatcggca ccctcaccgc gctggatttc
     3901 cttggcttcg gcctgccgcc tggcgcgccg tcactcggcg agctggtggc tcagggcaaa
     3961 tccaatctgc aggcgccctg gctcggtatc agcgcctttg ccgtgctcgc cctgatgctc
     4021 agcctgctgg tgtttatcgg cgaagcggcg cgcgatgcct tcgatccgag gaaatgacat
     4081 gaccgactcc aacaccctca tcgaagtccg tgacctggct gtcgaattcg tcaccggcag
     4141 cctgaaacag cgtgtgctgg aaggtgtcag cttcgatatc aagcgcggcg aaaccctggc
     4201 cctggtcggc gaaagcggct cgggcaaatc ggttaccgca cactcaatcc tgcgcctgct
     4261 gccctacccg ctggctcagc accctagcgg cagcattcac tacgctgggc aagacctgct
     4321 caaactaaac gagaacaaac tgcgcggcat tcgtggcaac cgcatcgcca tggtctttca
     4381 ggagcccatg acctcgctga atccgctgca cagcatcgag aagcagatca acgaggtgct
     4441 ggccctgcac aaaggcctgc gtggcaaagc agccaccgca cgtaccctgg agctgcttga
     4501 gctggtcggt attcccgaac cgcacaagcg cctcaagacc tacccacatg aactgtcagg
     4561 cggccaacgc cagcgcgtgg taatcgccat ggccctggcc aacgagccgg aactgctgat
     4621 cgccgacgaa ccaaccactg cgctggacgt caccgtgcag ctgaagatcc tcgagctgct
     4681 taaggacctg caggcgcgcc tgggcatggc gattctgctg atcagccacg acctcaacct
     4741 ggtgcgacgc attgcccatc gcgtctgcgt gatgcagcgc gggcatatcg tcgaacaggc
     4801 ggcctgcgaa acgctgttta aagacccaca acacccctac acccgtgagc tgctgggcgc
     4861 cgagcccacg ggtggcccgg tcgatcagcc acctggcaaa accatgctgg aaatcgacaa
     4921 cctgcgcgtc tggttcccga tcaaaaaggg tctgctgcgc cgcacggtcg accatgtcaa
     4981 agcagtggac ggcatcgact tcagcctgcc gcagggtcat accctgggca tcgtcggcga
     5041 aagcggctcg ggtaaatcca ccttgggtct ggcgattctg cgtctgctcg gcagccaggg
     5101 cggcatccgc ttccagggca aggccatcga cagcctgtca caagaggatg tacgcccgct
     5161 gcgccgacag atgcaggtgg tgtttcagga ccccttcggc agcctcagtc cgcgtatgtg
     5221 tgttggccag attgtcggcg agggcttgca gatccaccgc atgggcagcg aagccgagca
     5281 ggaacaggcg attatcgctg ccctagtgga agtgggcctg gacccggaaa ctcgccatcg
     5341 ctatccccac gagttctccg gtggccagcg ccaacgcatt gccatcgccc gcgccttggt
     5401 gctcaaaccc gcgctgatcc tgctggatga gccgacctcg gccctcgacc gcaccgtgca
     5461 gcgccaggtg gtcgagctgc tacgctcgct gcaggccaaa tacaacctga cctacctgtt
     5521 tatcagccac gacctcgcgg tagtcagggc cttgagtcac caggtgatgg tggtcaaaca
     5581 gggcaaagtg gtcgagcaag gcccggcggc acgtgtcttt gccgcgccac aggacatcta
     5641 tacacagcag ttgctggaag cggcctttat ggtcccagct gactaattcc ctgcagacag
     5701 gaagaggaac gacacaaatg ggttttctaa gcggcaagcg cgtactgatc gttggcgtcg
     5761 ccagcaaact gtccatcgcc tccggcatcg cggcagccat gcaccgtgaa ggcgccgaac
     5821 tggccttcac ctaccagaac gaaaagctca aaggccgcgt cgaaggcttt gccgctgatt
     5881 ggggctcagg tcctgagctg tgcttccctt gcgatgtggc caacgatgag gaaatcgcct
     5941 cggtattcga agccctgagc aagaagtggg acggtctgga ttgcatcgtt cactccgttg
     6001 gttttgcccc tggcgatcag ctggacggcg acttcaccga cgtcaccacc cgcgaaggct
     6061 tccgcatcgc tcacgacatc agtgcctaca gcttcgtcgc cctggccaaa gccggccgcg
     6121 agatgatgaa aggccgcaat ggcagcctgc tgaccctgtc ctacctgggc gccgagcgca
     6181 ccatgcccaa ctacaacgtt atgggcatgg ccaaagccag cctggaagcc ggtgtacgtt
     6241 acctggccgg cagcctcggc ccagaaggca cccgcgtcaa cgccatctcc gctggcccaa
     6301 tccgcaccct ggcggcctcc gggatcaaga gcttccgcaa gatgctcgct gccaacgaga
     6361 agcagacccc actgcgccgc aacgtgacca ttgatgaagt cggcaacgcc ggcgccttcc
     6421 tctgctccga tctggcctcg ggcgtcagcg gcgagatcat gtacgtcgac ggcggcttca
     6481 acaccaccgc catgggcgcc atggaagagt aaacagttaa atcgcagcaa taaaaaaggc
     6541 cgcttaatta agcggccttt ttattacgtc agcgatatcg atcagaaacg ttcgatatcc
     6601 gctgtctctt ccagttgctg gcggaacgct tcgaagtcct gctgaccgac gcgcgaggca
     6661 aggaagcggc tgtacatggc cttatcctca tccgacagcg tttgctgcgg ctggctcacg
     6721 ccattgagac ggatgatcat aaaatcaccg ttgctcaggc tgaggccggc gaaggtcggc
     6781 ttgtcggccg cttcaggctt gggcatgcgg aacaacgcct gcagcaccac cggctcaaca
     6841 ccttcctggc tacgagtggc cgcttcgacc acattccagc cttgcgcagc atcggcctgg
     6901 gtaaccggcg tctgaccgtc gcgcagagca gccaactgct cttcaccctt agctttcacg
     6961 gcttcgcttg cgcgaacctt ggtcagctgg gcgcggatgc tatcggcaac ctgctcaagc
     7021 ggcagctgct caggtttgtt gtgctcttta acgcgcacca ctaccacagt gttcgggtcg
     7081 agctcgatca cactgctgtt gctgccgtct tccagcactt catcgctgaa tgccgcctga
     7141 atcacctgac gattagcggt cagaccttca gtgccaccct gacgtccgaa cgcctcagta
     7201 gtcttgacct gcaggcccaa ttcctgagct ggctgggcca gatcagaagc ctcaaacgcg
     7261 gcgtcttcga ggtctttgga gacttcgaca aagcgctgct caacctgacg cgccttgagg
     7321 tcgcgctgca gtttctcttc caggctggcg aagctcggta cttcaggtgc ctgaacgccc
     7381 aggagcttaa tcaggtgcca gccgaactca ctgcgcactg gtgcagacac agcattctca
     7441 tccagcgcat acagcgcttc ttcaaaggct gggtcgtaca cgcccgggcc ggcaaagccc
     7501 agatcaccac cctcacctgc cgaacccgag tcatcggaga cttctttggc cagcgcggcg
     7561 aaatcttcgc cctgctccag acgtttctgc accgcgacca gtttggcctg ggcttgttcg
     7621 tcattcagct tatcgttgac ctcaaccaga atgtgcgcgg cacggcgctg ttcggcgagg
     7681 ttggcgattt cactctgata agcactttgc agctcttcat cggtgacttc aacctgatca
     7741 aagaaggcat ccttcttcaa ctcgacgtat tccagcacaa cctgctcggg gctcatgaac
     7801 tcgctagcct gagcgtcgta gtacgccttg atgtcgtcat cgctgagcgt gactgcatcc
     7861 agatcagctt tgagggtcag agtggcgaag tcgcgggtct gcttttccag ggcggcaaaa
     7921 gcttgtacct gagcatcagt gacaaaaccg ctgttggcca ggcctgcgcg cagctgaccg
     7981 atgagcattt cctgctcaag catctggcgg aactgcaagc ggctgtagcc cagctgacgg
     8041 ataacctgat cgaaacgctc agcgttgaac tgaccatcga caagaaactc aggtgttttc
     8101 aggatcagct gatccagcgc ctgttgcgaa aaggcgaact tggcgtcttt ggcgctatcg
     8161 agcagcaagg tacgctcaac cagaccattc agggcggctt cacgcagcag cttctcgtcc
     8221 agcagggagg catcgaaatc gcgccccaac tgttgcagca attgacgacg ctgcatctca
     8281 acagcctggc tgagctcggc ggaagtgatc ttctcgccat tcacttcagc ggcattctgg
     8341 ctattactgg tgctggtaaa aatggcatcc acacccgtca gcgcgagcag cgcgacaatg
     8401 atgccgatga ttgttttggc aatccaaccc tgtgaattgt ccctgatgtt ttgcagcatg
     8461 cgtcccccag aacgactgta caaataaagc agtcgcgcag tgtgggtaaa atccggatag
     8521 aagaaaggcg catcagtgga tgcgccttct cgtaactggc gaagcggatc gagctcaagc
     8581 aactgattcg ttgtgttcag cgaggtatgc ctgactgaac atcccgcctc gcgtcagatc
     8641 agccattcaa ctgatctgct gggcaaaacc gaaagaagct tagttgacag cgtctttcag
     8701 agctttaccg gctttgaagc ctggaatctt ggcagcagcg atgctgatcg ggttaccagt
     8761 ttgtgggtta cggccagtgc gagcagcacg ctatttaaca gcgaaagtgc cgaagccaac
     8821 cagtactacg gagtcaccag ccttcagagc gccagtaacg gattcaatta ctgcgtccag
     8881 cgcacggccg gcaacagctt ttgggatatc agcagatgca gcgatggcat cgatcagttc
     8941 cgac
//
LOCUS       DASRUM010000273         4257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_795198, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000273 DASRUM010000000
VERSION     DASRUM010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795198"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..2669)
                     /gene="acnD"
                     /locus_tag="VFF22_18300"
     CDS             complement(57..2669)
                     /gene="acnD"
                     /locus_tag="VFF22_18300"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HZX18807.1"
                     /translation="MNTQYRKPLPGTALDYFDTREAVDAIAPGAYAKLPYVSRVLAEQ
                     LVRRCEPAALTDSLKQLIERKRDLDFPWYPARVVCHDILGQTALVDLAGLRDAIAEKG
                     GDPSKVNPVVPTQLIVDHSLAVEAAGFDPDAFAKNRAIEDRRNEDRFHFIEWTKTAFK
                     NVDVIPAGNGIMHQINLEKMSPVIQARGGVAFPDTCVGTDSHTPHVDALGVIAIGVGG
                     LEAETVMLGLPSMMRLPDIVGVQLTGKRQPGITATDIVLAITEFLRKERVVGAWVEFF
                     GAGADSLSIGDRATISNMCPEYGATASMFYIDQQTIDYLKLTGREPEQIELVENYAKT
                     TGLWADALVSAEYERVLSFDLSTVVRNMAGPSNPHKRLPTSALQERGIADEAKLASGK
                     VEEAEGLLPDGAVIIAAITSCTNTSNPRNVVAAGLLAKKANELGLLRKPWVKTSFAPG
                     SKVAKLYLEDAGLLSELEKLGFGIVGYACTTCNGMSGALDPVIQQEIIDRDLYATAVL
                     SGNRNFDGRIHPYAKQAFLASPPLVVAYAIAGTVRFDIEQDVLGTDKAGNPITLKDLW
                     PSDEEIDSIVAASVKPEQFKQIYIPMFDLGTIEEAESPLYDWRPMSTYIRRPPYWEGA
                     LAGERTLKGMLPLAILPDNITTDHLSPSNAIQMNSAAGEYLHKMGLPEEDFNSYATHR
                     GDHLTAQRATFANKELVNEMAVVDGVVKKGSLARVEPEGKVMRMWEAIETYMNRKQNL
                     IIVAGADYGQGSSRDWAAKGVRLAGVEVIVAEGFERIHRTNLVGMGVLPVEFKPGTTR
                     LTLGLDGTETYDIQGDVSPRSDLTLVVNRRNGDVVKVPVICRLDTAAEVNVYNAGGVL
                     QRFAQDFLESTVAV"
     gene            complement(2684..3844)
                     /gene="prpC"
                     /locus_tag="VFF22_18305"
     CDS             complement(2684..3844)
                     /gene="prpC"
                     /locus_tag="VFF22_18305"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HZX18808.1"
                     /translation="MSATPETTPGFKPKKSVALSGTTAGNTALCTVGRTGNDLHYRGY
                     DVLDFANRCDFEEIAHLLVHGKLPNAVELAGYKAKLKALRGLPAALKVSLEQLPPSAH
                     PMDVMRTAVSVLGCVSPEKDDHNHPGARDIADKLMASLGSALLYWYHFSHNGKRIDVE
                     TDDDSIGGHFLHLLHGEKPRESWVRAMHTSLNLYAEHEFNASTFTSRVIAGTGSDMYS
                     CIAGGIGALRGPKHGGANEVAFEIQKRYDNPDEAEADIRARVEKKEVVIGFGHPVYTV
                     ADPRNKVIKEVARELSAEQSNSKMFDIAERLETIMWDIKKMFPNLDWFSAVSYHMMGV
                     PTAMFTPLFVIARTSGWSSHVIEQRIDGKIIRPSANYTGPEDLKFVPLKDRK"
     gene            complement(3913..>4257)
                     /gene="prpB"
                     /locus_tag="VFF22_18310"
     CDS             complement(3913..>4257)
                     /gene="prpB"
                     /locus_tag="VFF22_18310"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249487.1"
                     /note="catalyzes the formation of pyruvate and succinate
                     from 2-methylisocitrate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HZX18809.1"
                     /translation="GADMIFPEAITELAMYKTFADRVKAPILANITEFGATPLYTTEE
                     LASVDVGLVLYPLSAFRAMNKAAENVYTAVRRDGTQRNVVDTMQTRMELYERINYHAF
                     EQSLDALFAAKK"
BASE COUNT          771 a         1268 c         1354 g          864 t
ORIGIN      
        1 agccgcgatt tagctttaaa aagctcgcgg ctaaagcccc tcccacaaca ctgcgcttaa
       61 actgccaccg tagactcgag gaagtcctga gcaaagcgct gcagcacgcc gccagcgttg
      121 tagacgttga cttcggcagc ggtatccaga cggcagatca ccggcacctt gaccacgtca
      181 ccattgcgac ggttgaccac cagggtcagg tcgctgcgtg gcgacacatc accctggatg
      241 tcataggtct cggtgccgtc cagaccaagg gtcaggcggg tggtgcccgg cttgaactcg
      301 actggcagca cgcccatgcc gaccaggttg gtgcggtgga tgcgctcgaa gccttcggcg
      361 acgatcactt ccacgccagc caggcgcacg cccttggccg cccagtcacg cgacgagccc
      421 tgaccgtagt cagcaccggc aacgatgatc aggttctgtt tacggttcat gtaggtttcg
      481 atcgcttccc acatgcgcat gaccttgcct tccggctcga cacgggccag cgaacctttc
      541 ttcactacgc cgtcaacaac ggccatttca ttgaccagtt ctttgttggc gaaagtagcg
      601 cgctgcgccg tcaggtggtc gccgcggtga gtggcgtagg agttgaagtc ctcctcgggc
      661 aggcccatct tgtgcaggta ttcaccggcg gccgagttca tctggatcgc gttggacggc
      721 gacaggtggt cggtggtgat gttgtccggc aggattgcca gcggcagcat gcccttgagg
      781 gtacgctcac cggccagcgc gccttcccag taaggcggac ggcggatgta ggtggacatc
      841 gggcgccagt cgtacagcgg gctttccgct tcctcaatgg tgcccagatc gaacatcggg
      901 atatagatct gcttgaactg ctccggtttc accgaggcag caacgatgga gtcgatttcc
      961 tcgtcgctcg gccacagatc cttcagggtg atcgggttgc ccgctttatc ggtacccagc
     1021 acgtcctgct cgatatcaaa gcgcacggta ccggcgatgg cgtaggccac gaccagcggc
     1081 ggcgaggcca ggaaggcctg cttggcgtag gggtggatac gaccgtcgaa gttgcggtta
     1141 ccggacagta ccgcagtggc gtacaggtcg cggtcgatga tttcctgctg gattaccgga
     1201 tccagagcac cggacatgcc gttacaggtg gtgcaggcat agccgacaat gccgaagccg
     1261 agcttttcca gctccgacag cagaccggca tcttccaggt acagcttggc gaccttggag
     1321 cccggagcga aggacgtctt gacccacggc ttgcgcagca gacccagctc gttggccttc
     1381 ttcgccagca gaccggcggc caccacgttg cgcgggttgg aggtgttggt gcagctggtg
     1441 atggcggcga tgatcaccgc gccatctggc agcaggcctt cggcttcctc gaccttgccg
     1501 gaggccagct tggcttcgtc ggcaataccg cgctcttgca gtgccgaagt cggcaggcgc
     1561 ttgtgcgggt tggacgggcc agccatgttg cgcacaacgg tagacagatc aaagctcagt
     1621 acccgctcgt actcggcgct gaccagggca tcagcccaca gaccggtggt cttggcgtag
     1681 ttctctacca gctcgatctg ctccggctca cgaccggtga gcttaaggta atcgatggtt
     1741 tgctggtcga tgtagaacat cgaagcggtg gcgccgtatt ccgggcacat gttggaaatg
     1801 gtcgcacggt cgccgatcga caggctgtcc gcacccgcgc cgaagaactc gacccaggcg
     1861 ccgaccacgc gctccttgcg caagaactcg gtgatggcca ggactatgtc ggtggcggta
     1921 atgccaggct ggcgtttgcc agtgagctgc acgccgacga tgtcgggtag gcgcatcatc
     1981 gacggcaggc cgagcatcac agtttcggct tccaggccgc cgacgccgat ggcgatcacg
     2041 cccagagcat caacgtgcgg ggtgtgcgag tcggtaccca cgcaggtatc ggggaaggcc
     2101 acaccaccgc gcgcctggat caccgggctc attttctcca ggttgatctg gtgcatgatg
     2161 ccgttgccgg ccgggattac gtcgacgttc ttgaacgcgg tcttggtcca ctcaataaag
     2221 tggaaacggt cctcgttgcg acgatcttcg atggcgcggt tcttggcgaa agcatccggg
     2281 tcgaagcccg cggcctccac ggccagggag tggtcgacga tcagttgggt cggcaccacc
     2341 ggattgactt tggacgggtc accgcccttc tcggcgatgg catcacgcag gcctgccaga
     2401 tcgaccagcg cggtctgccc gagaatgtcg tggcagacca cccgcgccgg gtaccaaggg
     2461 aaatccagat cgcgcttgcg ctcgatcagt tgcttgagcg aatcggtcag ggcggccggc
     2521 tcgcagcggc gcaccaactg ctcggccagc acgcgggata cgtagggcag cttggcatag
     2581 gcgccggggg cgatggcatc caccgcctcg cgggtgtcga agtagtccag cgccgtgccg
     2641 ggcagaggct tgcggtactg agtattcagc agactgttca tgattatttg cgatccttga
     2701 gcggcacgaa cttgaggtct tcgggaccgg tgtagttggc gctcggacgg atgatcttgc
     2761 cgtcgatgcg ttgctcgatc acgtgggacg accagcccga ggtacgggca atcacgaaca
     2821 gcggggtgaa catggcggtg ggcacaccca tcatatggta gctgacggcg ctaaaccaat
     2881 cgaggttggg gaacattttc ttgatgtccc acatgatggt ttccaggcgc tcggcaatgt
     2941 cgaacatctt gctgttgctc tgctcggcgg acagctcacg cgccacttct ttgatcacct
     3001 tgttgcgcgg gtcggccacg gtgtacaccg ggtggccaaa gccaatcacc acttctttct
     3061 tctcgacgcg ggcacggata tcggcctcgg cctcatccgg attgtcgtag cgcttctgaa
     3121 tctcgaaggc cacttcgttg gcaccaccgt gcttgggccc acgcagcgcg ccgataccac
     3181 cggcaatgca gctgtacatg tcagaaccgg tgccggcgat cacccgtgag gtgaacgtgg
     3241 aggcgttgaa ctcgtgctcg gcatacagat tgagcgaggt gtgcatggcg cgcacccagg
     3301 actcgcgtgg cttctcaccg tgcagcagat gcaggaagtg gccgccgatg gagtcgtcat
     3361 cggtttccac atcgatgcgc ttgccgttgt ggctgaagtg ataccagtac aacaatgcgg
     3421 aacccagcga agccatcagc ttgtcggcga tatcccgggc gcccgggtgg ttgtgatcgt
     3481 ccttctccgg cgacacgcaa cccagcacgg atacggcggt gcgcatcaca tccatcggat
     3541 gggcggacgg cggcagttgc tcgagcgaga ccttcagtgc tgcaggcaaa ccacgcaggg
     3601 ccttgagttt ggccttgtag cccgccagtt ctacagcgtt tggcagcttg ccgtggacca
     3661 gcaggtgggc gatttcttcg aagtcgcagc gattggcgaa gtcgagcacg tcataaccgc
     3721 ggtaatgcag gtcgttgcca gtacggccga ccgtgcacag tgcggtgtta ccagccgtgg
     3781 taccgctcag ggccacggat tttttcggtt tgaagcctgg ggtggtttct ggggtcgcac
     3841 tcatcgctta tctctcctca tccgatccgg tcggtcgttc ttttaagcag gcggctaaga
     3901 cctgcgttcc ggttattttt tggcggcaaa cagcgcgtcg aggctctgct cgaaggcgtg
     3961 gtaattgatg cgctcgtaca gctccatgcg ggtttgcatg gtgtcgacca cgtttctctg
     4021 ggtgccgtcg cgacgcacgg ccgtgtagac gttctcggcc gccttgttca tggcgcgaaa
     4081 ggccgacagc gggtacagca ccaggccaac atccaccgag gccagctctt cggtggtgta
     4141 cagcggcgtg gcaccgaact cggtgatatt ggccagaatc ggcgctttca ctcggtcggc
     4201 gaaggtcttg tacatcgcca attcggtgat cgcttccggg aagatcatgt cggcacc
//
LOCUS       DASRUM010000274         8475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_478328, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000274 DASRUM010000000
VERSION     DASRUM010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8475
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478328"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3140
                     /locus_tag="VFF22_18315"
     CDS             <1..3140
                     /locus_tag="VFF22_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX18810.1"
                     /translation="EAEAEAEAEALPMIESFDLAEPELAELPEIEEEIDFGILADASQ
                     EPVDIEPLATESLEIESIELPAAEFDEQPLVEFDAEAEVEFAAIPEPTPSPVAAAAPA
                     VLDDDRDPELVEIFLEEGFDIIDSAGAALQRWTGDVNNSLELESLQRDLHTLKGGARM
                     AEIREIGDLAHELEFLYEGLGQGRLRASQDLFTLLQACHDSLAEMLEAVRGKRGVPSG
                     EALIETIKRFRANPDEQLSMPSSVALKAAETQQDDDAESDILDIFLEEGDDLLEAMEV
                     AIGRWEADREDGAAIDDMLRILHTLKGGARLAGQKRLGDLTHSLEQHLTEAQTQGAPW
                     PQSLFVDVQSGFEGLQQELDALRQRLNDSLAADTASQQAPAPVAEQASRLPELNAPIV
                     AASAMPAQELVAKVLPFVRRAEEAAQEAASRRAPQELVKVPAELLEGLVNLAGETSIF
                     RGRVEQQVSDFGFTLSEMEATIDRVRDQLRRLDTETQAQILSRIEAEAERMGYEDFDP
                     LEMDRHSQLQQLSRALFESASDLLDLKETLAARNRDAETLLLQQARVNSELQEGLMRT
                     RMVPFDRLVPRLRRIVRQISGELGKQVEFVVGNADGEMDRTVLERIVAPLEHMLRNAV
                     DHGIEAADVRRAAGKPEQGTIRLALGREGGDIVLTLADDGGGIRLDAVKRKAIERGLM
                     DADSDLTDYEVLQFILEAGFSTAEKVTQISGRGVGMDVVHSEVKQLGGSMSIESIPGT
                     GTTFTIRLPFTVSVNRALMVLSGEDLYAIPLNTIEGIVRVSPYELEAYYAPDAPRFEY
                     AGQSYELRYLGDLLNNGQQPKLVGQSLPLPVILVRSKEHAVAVQVDSLAGSREIVVKS
                     LGPQFAGVHGISGATILGDGRVVVILDLLATIRVLHAHLLTQLTPRLLKQSATAAEIE
                     ADRPTLVMVVDDSVTVRKVTSRLLERNGMNVLTAKDGVDAISQLQERKPDIMLLDIEM
                     PRMDGFEVATLVRHDEGLKDLPIIMITSRTGEKHRERAMNIGVNEYLGKPYQESLLLE
                     TIQQLVGRHPVNRNE"
     gene            3133..4152
                     /locus_tag="VFF22_18320"
     CDS             3133..4152
                     /locus_tag="VFF22_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HZX18811.1"
                     /translation="MSEKTAARIAVIADTSLQRHVLQQALTGSGYQVVMNSDPARLDQ
                     ETLNACETDLWLVDLAQSEDLPLVDSLMESASAPVLFGEGHAPERHSENYPRWERRLF
                     GKLKKLVGDPSQAVGPSLEALLAEAQRPSRLDLPAALADTPLVAGEPARQVWLLAASL
                     GGPAAVKAFLDALPGGLPVGFVYAQHIDASFETALPQAVGRHSQWHVNPARHGDAVRC
                     GEVVVVPISYELGFSDDGQMQISDRGWPEPYSPSIDQMMLNLAQHFGALSGVIAFSGM
                     GSDGSAAAAYVRRQGSVIWTQRADSCASPSMPDSLREGGYSSFSGDPRELAEALVNHL
                     AEQCR"
     gene            4184..4654
                     /locus_tag="VFF22_18325"
     CDS             4184..4654
                     /locus_tag="VFF22_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZX18812.1"
                     /translation="MIQAVATQNSTNSLTGLLMPLTDRTLLLPNVAVAELIPYRAPQV
                     SEGMPSWFLGQIAWRDLRLPLLSFEAASDGQVSVSPGSRVAVINALGGRPAVKFIAVL
                     VQGIPRSIKVGAELVRADVALSPLELDAVQFGEAVLKIPDLIGLEQKLADAGLI"
     gene            complement(4711..5430)
                     /locus_tag="VFF22_18330"
     CDS             complement(4711..5430)
                     /locus_tag="VFF22_18330"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019471551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HZX18813.1"
                     /translation="MRLSRFFIDAPLSLGQHELPEAQAHYIGRVLRHAVGDAVQLFDG
                     SGQEYRGELIEVGKKSVRVELREAFAGQAESPLHIHLGQGLSRGERMDWAIQKATELG
                     ANEISLIVSERCEVRLKDERADKRMAHWRQVAISACEQCGRSVLPIIHPPISLNDWLG
                     QVTAELKLVLHPVAEPWASHPQPKSLAFLIGPEGGLNESEVALAKTQGFHAARLGPRV
                     LRTETAPVVALSVAQQLWGDF"
     gene            complement(5515..6921)
                     /locus_tag="VFF22_18335"
     CDS             complement(5515..6921)
                     /locus_tag="VFF22_18335"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003303124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HZX18814.1"
                     /translation="MSLNDQWMQRDLKVLWHPCTQMKDHEQLPLVPIKRGEGVWLEDF
                     DGKRYLDAVSSWWVNVFGHANPRINQRIKDQVDQLEHVILAGFSHQPVIELSERLVKM
                     TPAGLDRVFYADNGSSCIEVALKMSFHYWLNVGKPAKKRFVTLSNSYHGETIAAMSVG
                     DVALFTDTYKALLLDTIKVPSPDCYHRPDGMSWEEHSRNMFAAMEQTLAEHSHEVAAV
                     IVEPLIQGAGGMRMYHPIYLKLLREACDRYGVHLIHDEIAVGFGRTGTMFACEQAGIR
                     PDFLCLSKALTGGYLPLAACLTTDAVYQAFYDDYSTLRAFLHSHSYTGNPLACAAALA
                     TLDIFEQDNVIEANKALATRMASATAHLADHPHVAEVRQTGMALAIEMVQDKASKTAY
                     PWQERRGLKVFQHALERGALLRPLGSVVYFLPPYVITPEQIDFLAEVASEGIDIATRT
                     SVSVAQGNANYPGFRDPG"
     gene            7223..7768
                     /locus_tag="VFF22_18340"
     CDS             7223..7768
                     /locus_tag="VFF22_18340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HZX18815.1"
                     /translation="MQWRNSSSHYGLVSILMHWLVAVVVFGLFGLGYWMVGLDYYSSW
                     RQTAPDLHKSIGLVLFAVMLTRVLWRWFSPPPASLPNHGRMTRLASKLGHSFLYIGLF
                     VLMISGYLISTADGRGIPVFGLFEVPASLTSIPDQEDVAGLVHEYLAWALVIFAGVHA
                     LAALKHHFIDRDRTLTRMFGR"
     gene            7829..8407
                     /locus_tag="VFF22_18345"
     CDS             7829..8407
                     /locus_tag="VFF22_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZX18816.1"
                     /translation="MLKKTLVALALGGALIGAGQAMAADYAIDKQGQHAFVNFKISHL
                     GYSWLYGTFKDFDGSFSFDAAKPQDSKVNVTLNTTSVDTNHAERDKHIRSDDFLNVSK
                     HPTATFASTSVKSTGEGTADITGDLTLNGVTKPVVIAAKFIGEGKDPWGGYRAGFEGS
                     TTLKLKDFDISKDLGPASESVELIISVEGVRK"
BASE COUNT         1600 a         2502 c         2666 g         1707 t
ORIGIN      
        1 ccgaagccga agccgaagcc gaagccgaag ccttgccgat gatcgagtcg ttcgacttgg
       61 cagagcccga gcttgctgag ctgcctgaga tagaagaaga gatcgacttt ggcatcctgg
      121 cggatgcgtc gcaggaaccg gtagatatcg aacccttggc gaccgagtcg ctggagatcg
      181 aatcgatcga gctgccggct gccgagttcg acgagcaacc actggtcgag tttgacgctg
      241 aagccgaagt cgagtttgct gcgatccccg agcccacccc atcgccggtc gctgctgcgg
      301 cacctgccgt acttgatgac gatcgcgatc cagaactggt cgaaatcttc cttgaggaag
      361 gtttcgacat catcgacagc gccggtgcgg cgttgcaacg ttggacgggc gacgtcaaca
      421 acagcctgga gttggagtcg ctacagcgtg atctgcacac cctcaagggt ggtgcacgca
      481 tggccgagat ccgtgaaatc ggtgacctgg cccacgagct ggaattcctc tacgaaggcc
      541 ttggtcaagg ccgtttgcgc gccagtcagg acctgtttac tctgcttcag gcttgtcacg
      601 acagcctggc cgagatgctc gaagcagtac gcggcaagcg cggcgtgccg agtggcgagg
      661 cgctgatcga aaccatcaag cggtttcgcg ccaacccgga tgagcagctg agcatgccgt
      721 cgtcggtagc cctcaaggcc gccgaaaccc agcaggatga cgatgccgaa tccgacatcc
      781 tcgacatctt ccttgaggaa ggtgatgacc tgctggaagc catggaagtg gctatcggcc
      841 gctgggaggc tgatcgtgaa gacggcgccg ccatcgacga tatgctgcgc attctgcaca
      901 ccctcaaagg tggtgcgcgt ttggctgggc agaagcgcct gggcgacctg acccacagcc
      961 tcgaacagca tctgaccgaa gcgcaaaccc agggcgcgcc ttggccgcag agcttgtttg
     1021 tcgatgtgca gtcgggcttc gaaggcttgc agcaggagct cgacgcactg cgtcagcggc
     1081 tgaatgacag cctggcggcc gatacggcga gccagcaagc gcctgcgcca gtcgccgagc
     1141 aagcctcgcg cctgccggaa ctgaacgccc cgattgttgc agccagtgcc atgccggcac
     1201 aggaactggt ggccaaagtg ctgccgtttg tccgtcgtgc tgaggaagca gcgcaggaag
     1261 cggcctcgcg gcgtgcgccg caggaattgg tcaaggtgcc ggccgaattg ctcgaaggtc
     1321 tggtcaacct ggccggtgag acatcgattt tccgtggccg ggtcgagcag caggtcagtg
     1381 acttcggttt caccctgagt gagatggaag ccaccatcga ccgcgtgcgc gatcagttgc
     1441 gtcgcctcga taccgaaacc caggcgcaaa ttctcagccg tattgaggct gaagccgagc
     1501 gtatgggcta tgaggatttc gacccgctgg aaatggaccg tcactcgcaa ttgcagcagc
     1561 tctcgcgcgc actgttcgag tccgcttccg acttgctcga cctgaaagaa accctggccg
     1621 cgcgtaaccg cgatgcggaa accctgttgt tgcaacaggc gcgggtcaac tccgaactgc
     1681 aggaaggcct aatgcgtacg cgcatggtgc cgttcgaccg tctggtgccg cgtctgcgcc
     1741 gtatcgtgcg gcagatttcc ggtgagctgg gcaagcaggt cgagtttgtc gtcggcaacg
     1801 ccgatggcga gatggaccgt accgtactcg aacgtatcgt cgcgccgctg gagcatatgc
     1861 tgcgtaacgc cgttgaccac ggcatcgaag ccgccgatgt gcgccgtgct gccggcaagc
     1921 cggagcaggg caccattcgc ctggcgctgg gccgcgaggg cggcgatatc gtcctgaccc
     1981 tggccgatga cggtggcggc attcgtctgg atgcggtcaa gcgtaaagcc atcgagcgcg
     2041 gtttgatgga cgccgactct gacctcaccg actacgaagt gctgcagttt attctcgagg
     2101 ccggtttctc cactgccgag aaggtcacgc agatttccgg gcgtggcgtg ggtatggacg
     2161 tggtgcactc cgaggtcaaa cagctcggcg gctcgatgag catcgagtcg atcccgggca
     2221 ctggcaccac atttaccatt cgcctgccgt tcactgtgtc ggtcaaccgc gcgttgatgg
     2281 tactgtcggg tgaagacctc tacgccatcc cgctgaacac catcgaaggt atagtgcggg
     2341 tttccccata cgagctggaa gcctactacg cgccggatgc gccacgcttc gagtacgctg
     2401 ggcagagcta tgaactgcgc tacctgggcg acctgctgaa caatggtcag caacccaaac
     2461 tggttggtca gagcctgcca ctgccagtga ttctggtgcg ctccaaggag cacgccgtag
     2521 ctgtgcaggt cgactccctc gccggttcgc gcgagatcgt ggtgaagagc ctcggcccgc
     2581 agttcgccgg tgtacacggg atttccggtg cgaccatcct cggtgacggt cgcgtggtgg
     2641 tcattctcga cctgctggcg actatccgcg tgctgcatgc gcatttgctt actcagctga
     2701 cgccgcgtct gctcaagcag tcggcaacgg cggcagaaat cgaagcggat cggccgaccc
     2761 tggtcatggt ggtcgacgac tcggttaccg tgcgcaaggt caccagtcgc ctgctcgagc
     2821 gaaacggcat gaacgtgctg accgccaagg acggtgtcga tgccatctcg cagctgcagg
     2881 agcgcaaacc cgacatcatg ctgctggaca tcgaaatgcc acgcatggac ggcttcgaag
     2941 tggcaaccct ggtacgccac gacgaaggtc tgaaagacct gccgatcatc atgattacct
     3001 cgcgtaccgg tgaaaaacac cgtgagcggg ccatgaacat tggcgtcaac gagtaccttg
     3061 gtaagcctta ccaagagtcc ttgttgcttg aaaccattca gcaactggtg ggccgccacc
     3121 cggtaaaccg caatgagtga gaaaactgca gcccgcatcg cggtgattgc cgatacctcg
     3181 ctgcagcgtc atgtgctgca gcaggcgttg accggcagtg gctatcaggt ggtgatgaac
     3241 agcgacccgg cgcgccttga tcaggaaacc ctgaacgcct gcgaaaccga cctgtggctg
     3301 gttgacctcg cccagtcgga agacttgccg ctggttgaca gcttgatgga gagcgcttcg
     3361 gctccggtgc tgtttggtga aggccacgca cctgagcgtc attcggaaaa ctacccgcgc
     3421 tgggaacgcc gtttgttcgg caagctgaag aagctggtcg gcgatccctc gcaggctgtc
     3481 ggcccgagcc tggaagccct gctggcggaa gcgcagcgcc catcgcgcct agatctgccg
     3541 gctgcgctgg cggatacccc gttggtagcc ggtgagccgg cgcgtcaggt atggctgctg
     3601 gcagcgtcct tgggcggtcc agcggcggta aaagcctttc tcgatgcctt gccgggcggt
     3661 ctgccggtgg ggttcgtgta tgcccagcat attgatgcga gtttcgagac tgcactgccg
     3721 caagcggtgg gtcgccacag tcagtggcat gtgaacccgg cgcggcatgg cgacgcggta
     3781 cgttgtggtg aagtggttgt agtaccgatc agttatgagc tgggcttcag cgatgatggc
     3841 cagatgcaga tttccgatcg cggctggcct gaaccctaca gcccctcgat tgatcagatg
     3901 atgcttaatc tggcccagca ttttggtgcg ctaagcggtg taatcgcctt cagtggcatg
     3961 ggcagcgacg gcagcgccgc agccgcctat gtgcgtcgcc agggcagcgt gatctggacg
     4021 cagcgcgcgg acagctgcgc cagcccgagt atgccggaca gcctgcgtga aggcggctat
     4081 agcagcttca gcggtgatcc gcgcgagctg gccgaagccc tggtcaatca cctggccgag
     4141 cagtgtcgat aatccggggc tgcgccctgt acaggaaatt tccatgatcc aagccgtcgc
     4201 cacgcagaac agcaccaaca gccttaccgg tctgttgatg ccgctgactg accgtactct
     4261 gttgctgcca aacgtcgcgg ttgccgagct gatcccttat cgcgcgccgc aggtcagtga
     4321 aggcatgccc agctggttcc tcgggcagat tgcctggcgt gatctgcgct tgccgctgct
     4381 gtcgttcgaa gctgcatcgg atggtcaggt cagcgttagc ccaggttctc gggttgcggt
     4441 gatcaacgcc ctgggcggtc gccctgctgt gaagtttatt gctgtgctgg tgcagggtat
     4501 tccgcgctcg atcaaggtcg gtgctgagct ggtgcgtgcc gatgtcgccc tgtcgccgct
     4561 ggagctggat gcggtgcagt ttggcgaagc ggtgttgaag attccagacc tgatcgggtt
     4621 ggagcagaag cttgcagacg ccggattgat ttgactggta ctgcacgaaa aagcccgcgt
     4681 ttgcgggctt tttcttgggc ggaaacttat ctagaaatcg ccccacaact gctgcgccac
     4741 gctcaatgcc actaccggtg cggtttccgt gcgcagcacg cgcgggccga ggcgagcggc
     4801 gtggaagccc tgggtcttgg ccagggcgac ttcgctctcg ttcagaccgc cttccgggcc
     4861 gatcagaaag gccagcgact taggctgcgg gtggctggcc cagggttccg caacggggtg
     4921 cagtaccaac ttgagttcgg cggttacctg ccccagccag tcgttcaggc tgatgggcgg
     4981 atgaatgatc ggtagcaccg agcggccgca ttgctcacag gcgctaattg ccacctgccg
     5041 ccagtgcgcc atgcgcttgt cggcgcgctc atccttgagg cgtacttcgc agcgctcgct
     5101 gacgatcagg ctgatttcat tggcgcccag ctctgtggct ttttgaatcg cccaatccat
     5161 ccgttcgccg cgcgacaggc cttggcccag atgaatatgc agcggtgatt ctgcctgacc
     5221 ggcaaaggct tcgcgcaact cgacgcgcac gcttttcttg ccaacctcga tcagctcgcc
     5281 gcggtattcc tggccgctgc catcgaatag ttgcaccgca tcgcccacgg catgccgcag
     5341 cacgcggccg atatagtggg cctgagcttc gggcagctcg tgctggccga gggagagggg
     5401 ggcatcgata aagaaacgag aaaggcgcat ggattgatcg ctgcagcggc acagttgagg
     5461 cggcaagctt aacgcctcag ccgtgtcgca tacagccggc tgagggtgag tgccttagcc
     5521 cggatcgcga aacccaggat agttggcatt gccttgcgcc acgctgaccg aggtgcgggt
     5581 ggcgatgtcg ataccttcgc tggccacttc cgcgagaaag tcgatctgct cgggggtgat
     5641 cacgtaaggc ggcaggaaat acaccacgct gcccagtggc cgaagcagtg cgccgcgctc
     5701 cagagcgtgc tggaacacct tgaggccgcg ccgttcctgc caggggtagg cggttttgct
     5761 ggctttgtct tggaccatct cgatggccag ggccatgccg gtctgacgta cttcggcgac
     5821 atgcgggtgg tcggccaggt gtgcagtggc gctggccatc cgcgtggcta acgccttgtt
     5881 ggcctcgatc acattgtcct gctcgaagat atccagggtc gcaagggctg ccgcgcaagc
     5941 cagcgggttg ccggtgtagc tgtgcgagtg gaggaaggcg cgcagggtcg agtagtcgtc
     6001 gtagaacgcc tggtacaccg cgtcggtggt cagacaggcg gccagcggca gatagccgcc
     6061 ggtaagcgct ttggacaggc agagaaaatc cgggcggatg ccggcctgct cgcaggcgaa
     6121 catcgttcca gtgcgaccga agcccacggc gatctcgtca tggatcaggt gcacgccgta
     6181 gcggtcgcag gcctcgcgca gcagcttgag gtagatcggg tggtacatgc gcatgccgcc
     6241 ggcgccctgg atcagcggct cgactattac cgccgcgact tcatggctat gttcggccag
     6301 tgtctgctcc atagcggcga acatattgcg cgaatgttcc tcccagctca tgccgtcggg
     6361 gcggtggtag caatccgggc tgggcacctt gatggtgtcg agcagcaacg ccttgtaggt
     6421 atcggtaaac agcgccacat cgcccaccga catcgccgcg atggtttcgc cgtggtagct
     6481 gttggagagg gtgacgaagc gcttcttcgc tggtttgccg acgttgagcc agtagtgaaa
     6541 gctcatcttc aatgcgactt cgatgcagga cgagccgttg tcggcgtaga acacccggtc
     6601 caggcccgcc ggggtcattt tgaccaggcg ttcggacaac tcgatcaccg gctgatggct
     6661 gaagccggcg agaatcacat gctccagctg atcgacctga tccttgatgc gctgattgat
     6721 ccgtgggttg gcatggccaa acacgttgac ccaccaggag ctgactgcat cgaggtagcg
     6781 cttgccgtca aagtcttcga gccatacgcc ttcgccgcgc ttgatcggca ccagcggcag
     6841 ctgctcgtgg tccttcattt gcgtgcaggg gtgccagagc actttcaggt cacgctgcat
     6901 ccactggtca ttcaggctca tcgctcgatt cattcctcgg ttcgcaatca tctgcgccaa
     6961 gcctatcaga agcaccgcag cggatggacg gcacaaccgc gctcaaatac ccaagcctcc
     7021 gctgatccgc tctatcatgc cgcctgtagc ctggcggctg aactttgccg agtaccgcta
     7081 gactaaattc gctgagttga aggggcgagt aacgcatcgt atttcccgat atttcaaagc
     7141 gaaaatttcc gcttttagtc gataggtttg ttgctagtct tgctccactc gcggtctcag
     7201 ttcttctttc ggaaagcttt caatgcaatg gcgtaatagc tcttctcact acggcctggt
     7261 cagcatcctc atgcactggc tggtggcggt ggtcgtgttc ggcctgtttg gtttgggtta
     7321 ctggatggtt ggcctggact actacagcag ctggcgccag accgcaccgg acttgcacaa
     7381 gagcatcggc ctggtgctgt ttgcggtaat gctgactcgg gtgttgtggc gctggtttag
     7441 cccgccgccg gcgagtttgc ccaatcatgg gcggatgacc cgcctggcca gtaaattggg
     7501 ccacagcttt ctctacattg ggctgtttgt gctgatgatt tccggctacc tgatctctac
     7561 ggcagacggt cgcggcattc cggtattcgg cttgtttgaa gtcccggcca gcctgaccag
     7621 cattcctgat caggaagacg tagccgggct ggtgcatgaa tacctggcgt gggcgctggt
     7681 gatcttcgcc ggtgtgcatg ccctggctgc gctgaaacac cactttattg atcgcgaccg
     7741 tacgcttacg cggatgttcg gtcgctagtt ccgtttgctg tttccggggt tgaattagcc
     7801 ccatcccaca ctcacaagga gcatgcccat gttgaagaaa accctcgtag ccctggcact
     7861 tggtggcgca ctaatcggcg ctggccaagc gatggcggct gattacgcca tcgacaagca
     7921 aggccagcac gccttcgtta atttcaagat cagccacctg ggctacagct ggctgtacgg
     7981 tacgttcaag gattttgacg gcagcttcag cttcgacgcc gccaagccgc aggacagcaa
     8041 ggtcaacgtg acgctgaaca ccaccagcgt cgacaccaac catgccgagc gcgacaagca
     8101 catccgcagc gatgacttcc tcaacgtcag taagcaccca actgcgacct tcgcgtccac
     8161 ttcggtcaaa tccactggtg aaggcactgc cgatatcacc ggcgacctga ccctcaatgg
     8221 cgtgaccaaa ccggtggtga ttgccgccaa gtttatcggt gaaggcaaag acccatgggg
     8281 cggctaccgt gctggcttcg agggcagcac cacgctgaag ctgaaggact tcgacatcag
     8341 caaagacctg ggcccagcct cggaaagcgt cgagctgatc atctcggttg aaggcgtacg
     8401 caagtaagct tgccaggtgg gacataaccc gcctgtttag acctgcgcca acaaaaacgc
     8461 cgcccaattg ggcgg
//
LOCUS       DASRUM010000275        13552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_312470, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000275 DASRUM010000000
VERSION     DASRUM010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 13552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13552
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312470"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /gene="fadD1"
                     /locus_tag="VFF22_18350"
     CDS             <1..523
                     /gene="fadD1"
                     /locus_tag="VFF22_18350"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD1"
                     /protein_id="HZX18817.1"
                     /translation="KVIDDEGVEQPLGSIGELCVKGPQVMKGYWQRQEATDEILDAEG
                     WLKTGDIAVIQEDGYMRIVDRKKDMILVSGFNVYPNELEDVLATLPGVLQCAAIGVPD
                     EKSGEAIKIFVVVKPGETLTKEQVMEHMRANVTGYKVPKAVEFRDVLPTTNVGKILRR
                     ELRDEELKKLGKK"
     gene            complement(621..992)
                     /locus_tag="VFF22_18355"
     CDS             complement(621..992)
                     /locus_tag="VFF22_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18818.1"
                     /translation="MRQPDIEIYLKDADHIAIGEWLNSALGNCTPWQQKGQTYKCMAG
                     EVPVTWLPKAVGKWNSLFLESDATPWADDLACAQAAFAALNVEVRCAPGGWQEEESDE
                     QADRWMRISADGVEEITWRTG"
     gene            complement(995..1798)
                     /locus_tag="VFF22_18360"
     CDS             complement(995..1798)
                     /locus_tag="VFF22_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017246240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZX18819.1"
                     /translation="MLICPICQAALQTLDNGVTCPANHRFDRARQGYLNLLPVQHKNS
                     RDPGDNAAMVEARRRFLDGGHYAPLAKRLAEMAAGYNPARWLDIGCGEGYYTAQIADA
                     LPQADGYALDISREAIKRGCKRAPQLNWLVASMARVPLSDASCQLLASVFSPLDWQEA
                     KRLLAPGGGLLRMGPTSEHLMELRQKLYDEVRDYDDQKHLELIPDGMRLAHSETLTFN
                     LHLQSSEARADLLAMTPHGWRASAEKRAAVIAEPCDVTVAIRYDWIERL"
     gene            complement(1945..3099)
                     /gene="dapE"
                     /locus_tag="VFF22_18365"
     CDS             complement(1945..3099)
                     /gene="dapE"
                     /locus_tag="VFF22_18365"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HZX18820.1"
                     /translation="MTANATPLSPTLELACELIRRPSVTPLDEGCQEQMTRRLAACGF
                     TIEPMRIEEVDNFWASHGSQEGPVLCFAGHTDVVPTGPVQNWQHQPFDALIDEQGMLC
                     GRGAADMKGSLASMIIAVERFVAEYPDHKGRIAFLITSDEEGPAHNGTKAVVERLRAR
                     NERLDWCIVGEPSSTTLVGDVVKNGRRGSLGCTLTVRGKQGHVAYPHLARNPIHLAAP
                     ALAELAAEHWDDGNTFFPPTSFQISNLNSGTGATNVIPGELKAIFNFRFSTESTVEGL
                     QQRTQAILDKHGLDFELDWALSGLPFLTEPGALLDAVAASIKAVTGRDTKPSTSGGTS
                     DGRFIATLGTQVVELGPVNATIHQVDERVLASDLDVLTEIYYRTLVNLLA"
     gene            complement(3193..5787)
                     /locus_tag="VFF22_18370"
     CDS             complement(3193..5787)
                     /locus_tag="VFF22_18370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZX18821.1"
                     /translation="MPSRKFGLNLVMFLAIAAVFTGIWALYNRPVTAPDWPEQISGFS
                     FSPFRAGQNPQQDTYPSDEQMRADLELLSTQTDNIRTYSVDGPLGNIPLLAEEFGLRV
                     TLGIWISPDLERNEREIVKAIEIANNSRSVVRVVVGNEALFRSEVTPEDLIAYIDRVR
                     AAIKVPVTTSEQWHIWQDHPELAQHVDLIAAHVLPYWEFVPMEDSTEFVLDRAKDLKK
                     LFPKKPLLLSEVGWPSNGRMRGGADASQADQAIYLRTLVNALNAQGYNYFVIEAFDQP
                     WKASDEGSVGAYWGVYNLERQAKFAFEGPVVAIPQWRLLAIGSVVLALLSLALMLIDG
                     SALRQRGRTFLTIVAFAGGSALVWIGYDYSQQYSTWFSTLVGLLLGIGAFGVFIVLLT
                     EAHELAETAWNSARRRPFQPVLADSAYRPKVSVHVPCYNEPPEMVKQTLDALAALDYP
                     DYEVIIIDNNTKDPAVWEPVEAYCKVLGPRFRFFHVAPIAGFKGGALNYILPHTAPDA
                     EVVAVIDADYCVDKNWLKYMVPHFSDPQIAVVQSPQDYRDGNENIFKKLCYAEYKGFF
                     HIGMVTRNDRDAIIQHGTMTMIRRTVMDELKWADWTICEDAELGLRVFEKGYSAAYAN
                     QSFGQGLMPDTFIDYKKQRFRWAYGAIQIMKGHARSLFLGKDSELKTGQRYHFVAGWL
                     PWIADGLNIFFTVGALLWSAAMIIVPQRVDPPLMIFAIPPLALFFFKLGKILFLYRKA
                     MGVDLVRSFQAAVAGLAMSHTIAKAVLYGTFTKTIPFFRNPKMASNHGILVALAEARE
                     EVFIMLLLWGAAAGISVVQGLPSADVQFWVAMLVVQSLPYLAALIMALLSSLPAPQAS
                     AQETVAAS"
     gene            6039..6854
                     /gene="tcdA"
                     /locus_tag="VFF22_18375"
     CDS             6039..6854
                     /gene="tcdA"
                     /locus_tag="VFF22_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014597143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HZX18822.1"
                     /translation="MPVDEQRFAGIARLYGRQGAERLAAAHVAVVGIGGVGSWAAEAL
                     ARSGVGEISLFDLDDVCVSNSNRQIHALDSTVGQPKVEVMAARIRAINPGCVVHAVTD
                     FVTRETMAEYITEQLDAVIDCIDSVNAKAALISWCKRRKIQVITTGGAGGQIDPTQIQ
                     VGDLNKTWNDPLASKVRSTLRRDYGFSRTPGRNYSVPCVYSSEQLRYPKPDGTVCQYK
                     GFVGEGVKLDCAGGFGAVMMVTATFGMVAAARIVDKLVAGTRRPAERPRSSGV"
     gene            6943..7749
                     /locus_tag="VFF22_18380"
     CDS             6943..7749
                     /locus_tag="VFF22_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX18823.1"
                     /translation="MLSLLRHLLSRTPRYVLLLAALSACLSAHAHPVVRVGTGDWVPY
                     VDQQREDGGALSRLVRDIFKAAGYQVEFVYYPWDRNLLMLEHGTLDAVMPYVCSPARQ
                     RFSTCSDAVVKGEVVLFQRADQQLDWSNIEDLKRFRIATSHGYSYGPQFDTAVQNGQL
                     QVQQSGKEDTGFRLLELGRVDFHPQDRAVGYAMLRRMFTPEARAAITHHPRMLNTEPL
                     HLLFRKGDTRTDQLRKTFNAGLRRLAQNGELERLQQALYSGNADQWRAAP"
     gene            complement(7728..8159)
                     /locus_tag="VFF22_18385"
     CDS             complement(7728..8159)
                     /locus_tag="VFF22_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HZX18824.1"
                     /translation="MSLPATAQTALQAFSACPGWEQRARLLMQWGDQLAPLSDAERCE
                     TNRVQGCESQVWLLAERDGAHWQFRAGSDARLLRGLLALLLARVNGLALEELAELDLP
                     DWFTQLGLARHLSPSRSNGLQAVLMRMQHLLDTVDQGAARH"
     gene            complement(8156..9361)
                     /locus_tag="VFF22_18390"
     CDS             complement(8156..9361)
                     /locus_tag="VFF22_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HZX18825.1"
                     /translation="MPIHSPWRADFPGILALEAEGQTYLDSAATAQKPQAMLDALLGY
                     YAGGAANVHRAQHLPGERATRAFEATREKAARWLNAPSAQQIIFTRSATEAFNLLAYG
                     LEQRLQPGDEIVISALEHHANLLPWQQLALRRQLKLVVLPLDQHGLIDLAQAAQLIGP
                     RTRLLAVSQLSNVLGCWQPLEPLLALAQAQGALTLVDGTQGVVHGRQDLQALGCDFYI
                     CSSHKLYGPDGVGLLYGRTHALEQLAHWQFGGEMLLHTDYTSASFRPAPLGFEAGTPP
                     IASVIALGASLDYLSNLDASTVSAHETALHSELLTGLRQRQGVRLLGTPTVALASFVV
                     DGVHNADLAHLLTEQGIAVRAGHHCAMPLLQGLGLSGAIRVSLGLYNDGNDLQRFFSA
                     LDQALELLQ"
     gene            complement(9539..10573)
                     /gene="dapD"
                     /locus_tag="VFF22_18395"
     CDS             complement(9539..10573)
                     /gene="dapD"
                     /locus_tag="VFF22_18395"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HZX18826.1"
                     /translation="MSTTLFSLAFGVGTQNRQGTWLEVFYAQPLLNPSSELVAKVVEK
                     LAYQGGNQAIAINNSQAGDLAEALKNVDSQQSALLTRLAESQKPLVVTLLAEDAVLTS
                     TPEAYLKLHLLSHRLVKPHGLNLTGIFPLLPNVAWTSQGAIDLGELAERQLEARLKGD
                     LLEVFSVDKFPKMTDYVVPAGVRIADSARIRLGAYVGEGTTVMHEGFVNFNAGTAGPG
                     MIEGRVSAGVFVGKGSDLGGGCSTMGTLSGGGNIVISVGEGCLIGANAGIGIPLGDRN
                     TVESGLYITAGTKVALLDADNKLVKVLKARDLAGQPDLLFRRNSQTGAVECKTHKSAI
                     ELNEALHAHN"
     gene            complement(10613..10960)
                     /locus_tag="VFF22_18400"
     CDS             complement(10613..10960)
                     /locus_tag="VFF22_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HZX18827.1"
                     /translation="MSKTLFGIKACDTMKKARTWLDEHGVSYAFHDYKACGIDRGNLE
                     KWCNEHGWETILNRAGTTFRKLDEAQKSDLDQHKAIELMLAQPSMIKRPVLDLGNKTL
                     VGFKADRYAAELV"
     gene            complement(10960..12156)
                     /gene="dapC"
                     /locus_tag="VFF22_18405"
     CDS             complement(10960..12156)
                     /gene="dapC"
                     /locus_tag="VFF22_18405"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HZX18828.1"
                     /translation="MNDALNQLQPYPFEKLRALLGSVQVAAGKSPIALSIGEPKHRSP
                     EFVAEALRANLDQLAVYPTTLGIPALREAIANWCNRRFGLPAGTLDPARHVLPVNGTR
                     EALFAFTQAVVKRDVDGLVVSPNPFYQIYEGAAFLAGAQPHYLPCLEEHGFNPDFDAV
                     SDEVWQRCQILFLCSPGNPTGALLPLPTLKKLIALADKFDFVIAADECYSELYFDEGN
                     PPAGLLTACAELGHHDFKRCVVFHSLSKRSNLPGLRSGFVAGDADILKSFLLYRTYHG
                     CAMPVQTQLASVAAWNDEAHVKANRDLYREKFDAVLATLEGVLDVQRPDGGFYLWAKT
                     PIDDETFTRELFAREHVTVVPGSYLSRAVDDNNPGAGRVRMALVAPLAECVAAAERIR
                     DFVKGL"
     gene            complement(12226..>13552)
                     /gene="glnD"
                     /locus_tag="VFF22_18410"
     CDS             complement(12226..>13552)
                     /gene="glnD"
                     /locus_tag="VFF22_18410"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HZX18829.1"
                     /translation="TVDAHTLNLIKHLRKFKWTELAEKFPLASKLIDKLPKPELIYMA
                     GLYHDIGKGRGGDHSELGAVDAEAFCVRHQLPNWDSKLIVWLVQHHLVMSTTAQRKDL
                     SDPQVIFDFARLVGDQTRLDYLYVLTVADINATNPSLWNSWRASLLRQLYTETKRALR
                     RGLENPLDREEQIRLTQIAALDILVRGGTDPDDAEQLWSQLGDDYFLRHTASDVAWHS
                     EAILQHANNSNPLVLIKETTQREFEGGTQIFIYAQDQHDFFAVTVAAMAQLNLNIHDA
                     RILTSTSQFTLDTYIVLEGEGGSIGENPARIKEIRQGLIDALKNPDDYPNIIQRRVPR
                     QLKHFAFAPQVTIHNDAHRPVTILEVTAPDRPGLLARIGRIFLDFDLSLQNAKIATLG
                     ERVEDVFFVTDAHNQPLSDPELCTRLQETMVAQLSDAPGLQIEQSRIII"
BASE COUNT         2461 a         3944 c         4465 g         2682 t
ORIGIN      
        1 caaagtgatc gacgacgaag gcgtcgagca gccgctgggt tcgattggtg agctgtgcgt
       61 caaaggcccg caggtgatga agggctactg gcagcgccag gaagccaccg acgaaatcct
      121 cgacgccgaa ggctggttga aaaccggcga catcgcggtg atccaagaag acggttacat
      181 gcgtatcgtc gaccgtaaga aggacatgat tctggtgtcc ggcttcaacg tctacccgaa
      241 cgagctggaa gacgtgctcg ctacgctgcc tggcgtgctg caatgcgcgg ccatcggtgt
      301 gccggatgag aagtcgggcg aggctatcaa gatcttcgtg gtggtcaagc cgggtgaaac
      361 tctgaccaaa gagcaggtca tggaacacat gcgcgccaac gtcaccggct acaaggtgcc
      421 gaaggccgtc gagttccgcg acgtattgcc gaccaccaac gtcggcaaga tcctgcgccg
      481 cgagctgcgt gatgaagagc tgaagaaact gggcaagaag taaacgcccg gtcaaacaaa
      541 accccgcctc ggcggggttt tgttgtttta ggggccatgc cgcgtttctg gtgcgcgcgg
      601 cgcaccctac ggggcagctg tcagccggtg cgccaggtga tttcttctac cccatcggcg
      661 ctgatgcgca tccagcggtc ggcttgttcg tcgctttctt cttcctgcca gccgccgggc
      721 gcgcagcgta cttcgacatt cagagcggcg aaggccgcct gggcgcaggc caggtcgtcg
      781 gcccagggcg tggcgtcgct ttccaggaac aggctgttcc acttgccgac agccttgggc
      841 agccaggtga caggcacttc accggccatg catttgtagg tctggccttt ctgctgccag
      901 ggggtgcagt tgcccagtgc gctgttcagc cactcgccga tggcgatgtg gtcggcgtct
      961 ttcaggtata tctcgatatc cggttggcgc atggtcatag tctctcgatc cagtcgtagc
     1021 ggatggcgac agtcacgtcg cagggttcgg cgatcactgc cgcgcgcttc tcggcgctgg
     1081 cgcgccagcc gtgtggggtc atggccagca ggtcggcgcg ggcctcgctg ctttgcaggt
     1141 gcagattaaa ggtcagggtt tcgctgtggg ccaggcgcat gccgtcgggg atcagctcca
     1201 ggtgcttctg atcatcgtag tcacgcactt cgtcatacag cttttgccgc aattccatca
     1261 gatgctcact ggtcggcccc atacgcagca ggccgccacc gggggcgagc aggcgtttgg
     1321 cctcctgcca gtcgagcggg ctgaagacgc tggccagcag ttggcagctg gcgtcactca
     1381 gcggcacgcg ggccatgctc gccaccagcc agttgagctg gggcgcacgc ttgcagccgc
     1441 gcttgatcgc ctcgcgggag atgtccaggg cgtagccgtc ggcttgcggc agggcgtcgg
     1501 cgatctgcgc ggtgtagtaa ccctcgccgc aaccgatatc cagccagcgc gccgggttat
     1561 aaccggccgc catttcggcc aggcgcttgg ctaatggtgc gtaatgacca ccgtcgagaa
     1621 atcgccgccg cgcctcgacc atggcggcgt tgtcgcccgg gtcgcggctg ttcttgtgct
     1681 gcaccggcag caggttcagg taaccctggc gcgcacggtc aaagcggtgg ttggccgggc
     1741 aggtgacgcc attatcgagc gtttgcagcg ccgcctggca gatagggcag atcagcatgc
     1801 gaaaaatcca ttaacaggcc gtcgcgagcg ccccgagagc aaggcggacg gaacgcagcg
     1861 acgagacata tcaaatagat aggcgaggag cgagtaccgc ccaacgcagc tatcggggtg
     1921 cgtagcaggc ttgttcatgg attttcatgc gagcagattg accagggttc ggtagtagat
     1981 ctcggtcagc acatccagat cactggccag tacgcgttca tctacctggt ggatggtggc
     2041 gttcaccggg ccgagttcga ccacctgggt gccgagggtg gcgataaagc gcccatccga
     2101 ggtgccgccg ctggtcgagg gcttggtgtc gcggccggtg acggccttga tgctggccgc
     2161 tacggcgtcg agcaaggcac ctggctcagt gagaaaaggc aggccggaga gtgcccagtc
     2221 gagctcaaaa tccaagccgt gtttgtcgag gatggcctga gtacgctgct gcaggccctc
     2281 aaccgtcgat tcagtcgaga agcggaagtt gaagatggct ttcaactcac ccggaatcac
     2341 gttggtcgcc ccggtgcccg agttgaggtt ggagatctgg aagctggtcg gcgggaagaa
     2401 cgtgttgccg tcgtcccaat gctcggcagc cagttcggcc agggccggcg cggccagatg
     2461 aatcgggttg cgcgccaggt gtgggtaggc cacgtggcct tgcttgccgc gcacagtgag
     2521 ggtgcagccg agcgagccac ggcggccgtt tttcaccacg tcaccgacca gggtggtgct
     2581 cgacggttca ccgacaatgc accagtccag gcgctcgttg cgtgcacgca ggcgctcgac
     2641 cacggccttg gtgccgttgt gcgccgggcc ttcttcgtcg ctggtgatca gaaaggcgat
     2701 acggcctttg tggtccgggt attcggcaac aaagcgctca acggcgatga tcatcgacgc
     2761 caggctacct ttcatgtccg ctgcgccacg accgcagagc atgccttgct cgtcgatcag
     2821 ggcatcgaat ggctggtgct gccagttttg tactgggccg gttggcacta catcggtgtg
     2881 tccggcaaag cacagtacgg gtccctcttg gctgccgtgg ctggcccaaa aattgtccac
     2941 ttcctcgatc cgcatcggct cgatggtgaa accgcaggcg gcaaggcggc gcgtcatctg
     3001 ttcctggcag ccttcatcga gcggcgtgac ggacgggcga cgaatcagct cgcaggcaag
     3061 ctccagcgtc ggggagaggg gcgtggcgtt ggcggtcatt gcggctccaa ggcggcatca
     3121 agcggaaagg ccgccatctt aaagcaaaac ggcggccctt ggccgccgtc tagcattcgg
     3181 gcatagccag tgtcagctgg cagcgaccgt ttcctgcgcg cttgcctgtg gcgctggcaa
     3241 ggacgacagc agcgccatga tcagcgccgc caggtagggc agcgactgca ccaccagcat
     3301 agccacccaa aactgcacat cggcgctggg caggccttgg acaacgctga tacctgcggc
     3361 cgctccccat agcagcagca tgataaagac ttcttcgcgc gcttcggcca atgccaccag
     3421 gatgccgtgg ttgctggcca tcttcgggtt gcggaagaac gggatggttt tggtaaaggt
     3481 gccgtagagc accgccttgg ctatggtgtg cgacatcgcg aggcccgcca ctgcagcctg
     3541 gaatgaacgc accagatcga cgcccatggc cttgcggtag aggaacagga tcttgcccag
     3601 cttgaagaag aacagcgcca atggcgggat ggcgaaaatc atcaacggcg ggtcgacccg
     3661 ctgcggcacg atgatcatcg ctgccgacca gagcagggcg ccgacggtga agaagatgtt
     3721 caggccgtcg gcaatccatg gcagccaacc cgcgacgaag tggtagcgct ggccggtctt
     3781 gagttcggaa tccttgccaa ggaacaggct gcgcgcatgg cccttcatga tctgaatggc
     3841 gccgtaggcc caacggaagc gctgcttctt gtagtcgata aaggtatccg gcatcagccc
     3901 ctgaccaaag ctctggttgg cataggctgc ggagtagcct ttctcgaaca cgcgcaggcc
     3961 cagttcggcg tcttcgcaga tggtccagtc ggcccacttc agttcgtcca ttaccgtgcg
     4021 gcggatcatg gtcatggtgc cgtgctggat tatcgcgtcg cggtcgttgc gcgtcaccat
     4081 gccgatatgg aagaagcctt tgtattcggc gtagcagagt ttcttgaaga tattttcgtt
     4141 gccgtcgcga taatcctgcg gcgattgcac cacggcgatc tgcgggtcac tgaagtgcgg
     4201 caccatgtac ttgagccagt tcttgtcgac gcagtagtcg gcgtcgatca ccgccaccac
     4261 ttcggcgtcc ggtgcggtgt gcggcaggat gtagttcagt gcgccgccct tgaaaccggc
     4321 aatcggcgcg acgtggaaga agcgaaagcg tgggcccagg actttgcagt aggcctctac
     4381 cggctcccac accgccgggt ccttggtgtt gttgtcgatg atgatgactt cgtaatccgg
     4441 gtaatccagt gcggccaggg cgtcgagggt ctgtttgacc atctccggtg gctcgttgta
     4501 gcagggcacg tggaccgaca ccttgggccg gtaagcactg tcggccaaga ccggctggaa
     4561 cggccggcgg cgcgcgctgt tccaggcggt ttctgccagt tcgtgggctt cagtcagtag
     4621 cacgataaat acaccgaagg caccgatacc cagcaacagg ccgaccaggg tgctgaacca
     4681 ggtgctgtat tgctggctgt agtcgtagcc gatccacacc agggccgagc cgccggcgaa
     4741 ggcgacaatg gtcaggaagg tgcgcccgcg ctggcgcaag gcgctgccgt cgatcagcat
     4801 cagcgccagg ctgagcagtg ccagcaccac cgagcctatg gcaagcaagc gccactgagg
     4861 aatcgccacc accggccctt cgaaggcgaa cttggcctgg cgctccaggt tgtatacgcc
     4921 ccaatacgca cccaccgagc cttcatcgct ggccttccac ggctggtcga aggcttcgat
     4981 aacgaaatag ttgtagccct gggcgttcag tgcattgacc agggtgcgca ggtagatcgc
     5041 ctggtctgcc tgtgaggcat cggcgccgcc gcgcatgcgg ccgttgctcg gccagccgac
     5101 ttcggaaagt agcaggggtt ttttggggaa cagctttttc aggtccttgg cgcgatcgag
     5161 gacgaactcg gtagagtcct ccatcggcac aaattcccag tagggcagta cgtgggcggc
     5221 gatcaggtcg acgtgctgag ccagttccgg atgatcctgc cagatgtgcc actgctcgga
     5281 ggtggtcacc ggcaccttga tcgccgcgcg tacgcggtcg atataggcaa tcaggtcctc
     5341 tggggttact tcgctgcgga acagggcttc gttaccgacc accacgcgca ccacgctgcg
     5401 tgagttgttg gcaatttcga tggctttgac gatttcccgc tcgttgcgct ccaagtccgg
     5461 actgatccag atacccaggg ttacgcgcag gccgaactct tcggccagca acgggatatt
     5521 gcccagcggg ccgtctaccg agtaggtgcg gatgttgtcg gtctgcgtgc ttagcaactc
     5581 caggtcggca cgcatttgct catcgctggg gtaggtgtcc tgctgcgggt tctgccctgc
     5641 gcggaagggt gaaaatgaaa acccggagat ttgttccggc cagtccggtg cggttaccgg
     5701 gcggttgtac agcgcccaga taccggtaaa cacggcggca attgccagga acatgaccag
     5761 gttcagtcca aacttacgcg aaggcatagt gtgcaggtcc agcgaagggg caataaaaag
     5821 cgacgagcgt cgggtggcgc atgctacgcc gccgccattg ggctgtatag cgtggtgggt
     5881 cggcatattg atgggacgat gggcggcctt gaaggttccg ctgatggcta tgtgctactg
     5941 ctgttcatcg gttagatcgg tctagaccca gacagtgctt gggcacagct tgccgactac
     6001 ctataatgcg cgccgcttgt ggcttgcgag agagaagtat gcctgtggat gaacaacgct
     6061 ttgccggaat cgcccgtctg tatggccgcc agggagccga acggctggcg gctgcgcatg
     6121 tggcggtggt tggtattggc ggtgtgggct cctgggctgc ggaagctttg gcgcgttcag
     6181 gcgtgggcga aatctcgctg tttgatctgg atgatgtctg cgtcagcaac agcaaccgtc
     6241 agattcatgc gctcgacagc acggttggtc agcccaaggt tgaggtgatg gcggcgcgca
     6301 ttcgcgcaat caatccgggc tgcgtggtgc atgcggtgac ggatttcgtc acccgcgaga
     6361 ccatggccga gtacatcacc gagcaactcg atgcggtgat cgattgcatc gacagcgtta
     6421 acgccaaggc cgcgctaatt tcctggtgca aacggcgcaa gattcaggtg attaccaccg
     6481 gcggcgcggg cgggcagatc gacccgacgc agattcaggt cggcgacctg aacaaaacct
     6541 ggaacgatcc gctggcctcc aaggtgcgtt ccaccctgcg ccgcgactac ggcttctcgc
     6601 gtaccccggg gcgcaattac agcgtgccct gcgtgtattc cagcgagcag ctgcgctatc
     6661 ccaagcccga cggcacggtg tgtcagtaca agggctttgt cggcgaaggg gtcaagctcg
     6721 actgtgccgg cggttttggc gcggtgatga tggtgaccgc caccttcggc atggtggccg
     6781 ctgcgcgcat cgtcgataaa ttggtcgccg gtacacggcg gcctgcggag cggccgcgct
     6841 cgtctggcgt atagcggtgc gtgtggatca atctgtaaaa acgaccggtt aaggtcgctg
     6901 ttcagcaggg atatgctgtt ttccgcttta tcgaggtgtg ttgtgttgtc cctgctgcgt
     6961 catctgttaa gtcgtacccc gcgttacgtg ctgctgcttg cagcgctttc tgcttgcctg
     7021 agtgcccatg ctcatccggt tgtacgggtt ggcaccggcg actgggtgcc ctatgtcgac
     7081 caacaacgcg aagatggcgg tgccttgtcg cgcctggtgc gcgacatctt caaggcggcg
     7141 ggttatcagg tcgaattcgt ctactacccc tgggaccgca acctgctgat gctcgagcac
     7201 ggcactctcg atgcggtgat gccatatgtc tgctcgccgg cgcggcagcg ttttagtacc
     7261 tgcagtgatg ccgtggtgaa aggagaggtg gtgctgttcc agcgtgctga ccagcagttg
     7321 gactggtcaa atatcgaaga cctcaagcga ttccgcatcg ccaccagcca cggctactcc
     7381 tacggtccgc aatttgatac agcggtgcag aacggacagt tgcaggtgca gcaaagcggc
     7441 aaggaagaca ccgggtttcg cctgctggaa ctgggccgcg tagattttca tccgcaagac
     7501 cgcgccgtgg gctatgccat gctgcggcgg atgtttaccc ccgaagcgcg cgcggccatt
     7561 acccatcatc cgcgtatgct gaataccgag ccgttgcacc tgctgtttcg caaaggcgat
     7621 acccgaaccg atcagctgcg taagacattc aatgccggtt tgcgccgctt ggcgcagaac
     7681 ggtgagttgg agcgtttgca gcaggccttg tattccggca atgccgatca gtggcgcgct
     7741 gcaccctgat cgactgtatc gagcaagtgt tgcatccgca tcagtacggc ctgcaggcca
     7801 ttgctgcgtg agggcgacag gtggcgggcc aggccgagtt gggtaaacca gtccggcaga
     7861 tccagctctg ctagttcctc caaggccagg ccattgaccc gcgccagcag cagcgccagc
     7921 agaccacgca atagtcgagc atcactgccg gcgcgaaact gccagtgcgc gccgtcacgc
     7981 tcggccagca gccacacctg actctcgcag ccttgcaccc ggttggtttc acagcgctct
     8041 gcgtcactga gcggggcaag ctggtcgccc cattgcatca gcaggcgcgc gcgctgttcc
     8101 caaccggggc aggcgctaaa ggcttgcagc gctgtctgcg cagtggcagg caggctcact
     8161 gcaacagctc cagggcctga tccagggcgc tgaagaaacg ctgcaggtcg ttgccatcgt
     8221 tatacagccc cagcgacaca cgaatcgccc cgctcaggcc gaggccctgc aatagcggca
     8281 tggcgcagtg atggccggcg cgtacggcaa taccctgttc ggtcagcagg tgggcgaggt
     8341 cggcgttatg cacgccgtcg accacaaagc tggccagggc gacagtgggt gtgccgagca
     8401 ggcgcacgcc ctggcgctgt cgcagccctg taagtagctc gctatgcagg gctgtttcgt
     8461 gggcgctaac ggtgctggcg tcgagattgc tcaggtaatc caggctggcg ccaagggcga
     8521 tcaccgaggc aatcggtggc gtaccggcct caaagcccag cggcgcgggg cgaaagctgg
     8581 cgctggtgta gtcggtgtgc agcagcattt cgccgccaaa ctgccaatgc gccagctgct
     8641 ccaatgcgtg cgtgcggcca taaagcaggc cgacgccatc cgggccatag agcttgtggc
     8701 tggagcagat atagaagtcg cagcccaggg cctgcaggtc ctggcgaccg tgcaccacac
     8761 cctgggtgcc atcgaccagg gtcagcgcac cttgcgcctg tgccagcgcc aacaatggct
     8821 ccagcggctg ccagcaaccg agcacgttgg acagctggct gaccgccaac aggcgggtgc
     8881 gcggcccgat cagctgcgcc gcttgggcta gatcgatcaa tccatgctga tccagcggta
     8941 acacaaccag tttgagctgc cgacgcagtg ccagttgctg ccagggcagt agattggcgt
     9001 ggtgttccag ggcgctgatg acgatttcgt cgccaggttg tagacgctgc tccaggccgt
     9061 aggccagcag gttgaaggct tcggtggcgc tgcgagtaaa gatgatctgc tgcgcgctgg
     9121 gcgcatttag ccagcgtgca gccttctcac gtgttgcttc gaaggcgcgg gtggcgcgct
     9181 cgcccggcag gtgctgggcg cgatgcacat tggctgcgcc gccagcgtaa tagccgagca
     9241 gggcatcgag catggcctga ggtttttgcg cggtggcggc gctgtccaga taggtttggc
     9301 cttctgcttc gagggccaga atgccgggga agtcagcgcg ccagggggaa tggataggca
     9361 tgggcaggca atcgagaccg taggatgggt tgagcgcagc gatacccata atgagcgtga
     9421 tgggtatcgc aatgctcaac ccatcctaca aaactatccc gtagggtgcg ccgtgcgcac
     9481 cggggcgcac atgggtttgt ggtgcgcgcg gcgcacccta cggtcaggca tttctagatc
     9541 aattatgggc gtgcagggct tcgttcagct cgatcgccga tttgtgggtc ttgcactcca
     9601 ctgcgccggt ttgcgagttg cggcggaaca gcaggtccgg ctggccagcc agatcgcggg
     9661 ccttgagtac cttgaccagt ttgttgtcag catcgagcag tgccaccttg gtgccggcag
     9721 tgatgtacag gccggactcc acggtgttgc ggtcgcccaa tgggatgccg ataccggcgt
     9781 tggcgccgat caggcagcct tcgcccaccg aaatcacgat attaccgcca ccggacaggg
     9841 tgcccatggt cgagcagccg ccgcccaggt ccgagccctt gccgacgaat acgcccgcag
     9901 aaacgcggcc ttcgatcatg cctgggccgg cggtgccagc gttgaagttg acgaagcctt
     9961 cgtgcatcac ggtggtgcct tcacccacgt aggcgcccag gcggatgcgt gcgctgtcag
    10021 cgatacgcac gccagccggt accacgtagt cggtcatttt cgggaacttg tccaccgaga
    10081 agacttccag caggtcgccc ttcaggcgcg cttccagttg acgctcagcc agttcgccga
    10141 ggtcgattgc gccctggctg gtccaggcga cgttcggcag cagcgggaag ataccggtga
    10201 ggttcaggcc gtgcggcttg accaggcggt gcgacagcag gtgcagcttg aggtaggcct
    10261 caggcgtgct ggtcagcacg gcgtcttcgg cgagcagggt gaccaccagc ggcttctggc
    10321 tttcggccag gcgggtgagc agggcggact gctggctgtc gacatttttc agtgcttcgg
    10381 ccagatcgcc agcctggctg ttgttgatcg caatggcctg gttaccgccc tggtaagcga
    10441 gtttttccac cactttggcg accagttcgc tgctcgggtt gagcagtggc tgggcgtaga
    10501 agacttccag ccaggtgccc tgacggttct gggtgccaac gccgaaagcc aggctgaaga
    10561 gggttgtgct catgctattt tccttaccgc aaattcgtgt gggccggctg gttcaaacca
    10621 gttcggcggc ataacggtcg gctttgaagc caaccagggt cttgttgccg agatccagca
    10681 ccgggcgttt gatcatcgac ggttgggcga gcatcagttc gatcgccttg tgctgatcga
    10741 ggtcgctttt ctgcgcctcg tccagcttgc gaaaggtggt gcctgcacgg ttcaggatgg
    10801 tttcccaacc atgctcattg caccactttt ccaggttgcc acggtctatg ccgcaggctt
    10861 tgtaatcatg aaaggcgtag cttaccccgt gctcgtcgag ccaggtgcgg gcttttttca
    10921 tggtgtcgca ggcttttatg ccaaacaggg tcttgctcat cagaggccct tcacaaagtc
    10981 gcggatacgt tcggctgccg ctacgcactc ggccagcggc gcgaccaggg ccatgcgcac
    11041 acggccggcg cctgggttgt tgtcgtctac tgcgcgcgac aggtacgagc caggcaccac
    11101 ggtgacatgc tcgcgggcaa acagctcgcg ggtaaatgtt tcatcgtcta tcggggtttt
    11161 cgcccacagg tagaagccgc catccgggcg ctgcacatcc agcacgcctt ccagagtcgc
    11221 cagcaccgcg tcgaacttct cgcggtacag gtcgcggttg gctttgacat gggcttcgtc
    11281 gttccaggcg gctacgctgg ccagctgagt ttgtaccggc atggcgcagc cgtggtaggt
    11341 acggtacagc aggaaagatt tcagaatgtc ggcgtcgccg gcgacgaagc cggaacgcag
    11401 gcctggcagg ttggagcgct tggacaggct gtggaacacc acgcagcgtt tgaaatcatg
    11461 gtggcccaat tcggcgcagg cggtcagcag gccggccggc gggttgcctt catcgaagta
    11521 cagctcgctg tagcactcat ccgcagcgat gacgaagtcg aatttgtcgg cgagggcgat
    11581 cagcttcttc agcgtcggca gcggaagcag cgcgccggtc gggttgccgg gcgagcagag
    11641 aaagaggatc tggcaacgct gccagacctc gtcgctgacg gcgtcgaaat ccgggttgaa
    11701 accgtgttct tccaagcatg gcagatagtg cggctgcgcg ccggcaagaa aagcggcgcc
    11761 ttcatagatc tgatagaagg ggttggggct gactactagt ccatcgacat cgcgcttgac
    11821 caccgcctgg gtaaaagcaa acagcgcctc acgggtgccg ttgaccggca gcacatggcg
    11881 cgctggatcg agcgtaccgg ccggcagacc aaagcggcga ttgcaccagt tggcgatggc
    11941 ttcacgcagt gccggaatgc ccagggtagt cgggtagacc gctagctggt caaggttggc
    12001 gcgcagcgcc tcagcgacaa actccggcga gcggtgtttc ggctcgccga tcgacagggc
    12061 aatcggactt ttcccggcgg ctacctgcac gctgcccagc agggcgcgga gcttttcgaa
    12121 cgggtagggc tgcagctggt ttaacgcgtc gttcattgtt gcttcctgag ttgtgccagc
    12181 gcccgcatcg tagatgtcgg gcggctcaag gtaagggcaa gtggtttaaa taatgattcg
    12241 gctctgttcg atctgcaggc ctggcgcgtc ggagagctgc gcgaccatgg tttcctgcag
    12301 gcgcgtgcac agctcggggt cggacagtgg ctggttatgc gcatcggtga cgaagaacac
    12361 gtcttcgacc cgctcaccca gggtggcgat cttggcgttc tgcagcgaca ggtcgaagtc
    12421 gaggaaaatc cgcccaatcc gcgccagcaa acccggtcga tccggagcgg tgacttcgag
    12481 aatggtcact ggccgatgcg catcgttgtg aatggtcacc tgcggtgcga aggcaaagtg
    12541 cttgagctgg cgcggtacgc ggcgctgaat gatgttcggg taatcatccg ggttcttcag
    12601 cgcgtcgatc aggccctggc gaatctcctt gattcgcgcc gggttctcgc caatcgagcc
    12661 gccttcgcct tcgagcacga tgtaggtgtc gagggtgaac tggctggtgg aggttagaat
    12721 ccgcgcgtca tggatgttca ggttgagctg ggccatggcc gccacggtca cggcgaagaa
    12781 gtcgtgctga tcctgggcat agataaatat ctgtgtgccg ccctcgaact cgcgctgggt
    12841 ggtttccttt atcagcacta gcgggttgct gttgttggcg tgctgcagga tcgcctcgct
    12901 gtgccaggcc acgtcgctgg ccgtgtggcg caggaagtag tcgtcaccca gttgcgacca
    12961 tagctgctcg gcgtcgtccg ggtcagtgcc gccgcgcacc agaatgtcca gcgcggcgat
    13021 ctgcgtcagg cgaatctgct cttcgcggtc cagcgggttt tccaggccac ggcgcagcgc
    13081 acgcttggtc tcggtgtaga gctggcgcag caggctagcg cgccaggaat tccataggct
    13141 ggggttggtg gcgttgatgt cggccacggt cagcacgtag aggtaatcca gacgggtctg
    13201 atcgccaacc aggcgggcga aatcgaagat cacctgcggg tcggagaggt ccttgcgctg
    13261 cgccgtggtg gacatcacca ggtgatgttg caccagccag acgatcagct tggagtccca
    13321 gtttggcagc tggtggcgta cacagaaggc ctcagcatcc actgcgccga gttccgaatg
    13381 gtcgccgcca cggcctttgc cgatgtcgtg gtacagcccg gccatataga tcagctcggg
    13441 cttgggcagc ttgtcgatca gtttgctggc cagggggaat ttttccgcca gctcggtcca
    13501 cttgaactta cgcaggtgct tgatcaggtt cagggtgtgc gcatcgaccg ta
//
LOCUS       DASRUM010000276        26781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1328407, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000276 DASRUM010000000
VERSION     DASRUM010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 26781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26781
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328407"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFF22_18415"
     CDS             <1..223
                     /locus_tag="VFF22_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016986992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosomal subunit interface protein"
                     /protein_id="HZX18830.1"
                     /translation="GLQPISVTHKAESLEQAIDGSIEKLHHALEHQFGKLRSKRVTPL
                     HADEANDIPGQDALLEEDFLAEEQLRAVN"
     gene            466..939
                     /locus_tag="VFF22_18420"
     CDS             466..939
                     /locus_tag="VFF22_18420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18831.1"
                     /translation="MTSISNHSPLASYFANTAKPSTAGAAGSGSSTDKPNTSALDPLA
                     EIRRFASGMVANSRGGLLRAMHAESSGASNAIASDRFRPVAEPESDISKIVLPDVAEL
                     DREDAAKLLAQINKVVDAGLDKTDSFVGYNGDQQTDSLTTYRDWLQARGGVSIYV"
     gene            1064..1915
                     /locus_tag="VFF22_18425"
     CDS             1064..1915
                     /locus_tag="VFF22_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZX18832.1"
                     /translation="MASKEFIRAFAALGLALLCATGAQAAEDAQLIGLINSYRSEVQR
                     CGDQASAELPPLTADPRLVQPVGRSGDLQQAMSAAGYPMVNVQAISLSGPRDAAAALK
                     VLQESFCKIVLDPQFVDIGVNQQDREWRILLARPLLAARLGDWQAEGQRLLEQLNSAR
                     GTARQCGGQAFAAAAPLVWNATLGSTAEAHSRAMANGNFFDHQDPDGRTPGDRAELAG
                     YSGQRVGENIAAGLDNASKVLAGWLASPGHCANLMNPQFSELGAAYANDPKSDAGIYW
                     TALFGAP"
     gene            complement(2037..3683)
                     /locus_tag="VFF22_18430"
     CDS             complement(2037..3683)
                     /locus_tag="VFF22_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX18833.1"
                     /translation="MRLDSLTLKTRLIIAVAIPCIALILVAVTSLSSMSTMQQQASAL
                     YLNTSAPMRAMAETASRIPRMRVGIDMMLLQDTGLRDERGVKTRVQEALNEDIPGMRE
                     AMRHAVDAQVNPDRKAQAERLLQQFESMVSNELTPLLQALDAGDMGRAQQIYRDQYAK
                     SYGVMRLGANELLDGLLQQAEQQNQLSNESYVNGRSRQLIIITLGLLASFITAWLIVS
                     NLRRRVSILQDKLGNAAENLSLNTRIELPGEDELSQIANSFNRFIAKVHSTMEQLAGN
                     SRQLSAMAREVAERARLTQSNCTSQSDRTVQVATAIHELGSTVNEIAGNAENAAHVAH
                     EASRHASDGRDVVAQAHQQINTLSGELEQASNVVEALAGQITAISSTLNTIRSISEQT
                     NLLALNAAIEAARAGEQGRGFAVVADEVRTLASRSGASTEEIQQVINRLQAESRAAVD
                     VMSRGRQQSERVVEFAGKASAALEQINGHITQISDQNIQVATATEEQSCVVEEINRNV
                     EEINNLTQETTQIADQLSQSSTSLQDLSTRLDELVGHFKL"
     gene            3904..4155
                     /locus_tag="VFF22_18435"
                     /pseudo
     CDS             3904..4155
                     /locus_tag="VFF22_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281460.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(4208..5542)
                     /locus_tag="VFF22_18440"
     CDS             complement(4208..5542)
                     /locus_tag="VFF22_18440"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZX18834.1"
                     /translation="MTSNPHLPHDKLLDLLLDVVCVVDKDGRFLSVSAASERVFGYRP
                     EEMIGRSAFELMHPDDRAASRALVKSINSGQPSSDHENRYIHKDGHIVHIMWSAQWLE
                     AEQVRVGVARDITRRKRAEAVQAALYAVSEAAHAVGDLHRLYEQVHEVVAQLLPAQHF
                     CVALHDAASDQLSVAYPLAPDDSPIATRDFCAEVIRAGRALLLTPETAPQGTATATHA
                     DWLGVPLISGTQPLGALVLQNHSASVRYNEQDQELLQFVSNQVAAAIERKRTHSRLEF
                     LAQYDQLTELSNRTLFLDRLRSALQRAKRDSLRLAVLYLDMDNFKQINDTYGHSAGDL
                     LLQEVARRLLQCVRESDTVGRLGGDEFAVIVDNIGRQEDATLVAEKIIAALSAPYLLD
                     GHHLISAPSLGIAVHPDHGQSELQLVQQADEAMYVAKRSGGKRYVVVEKPLK"
     gene            5755..6438
                     /locus_tag="VFF22_18445"
     CDS             5755..6438
                     /locus_tag="VFF22_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZX18835.1"
                     /translation="MHVLIVEDNPDIIANLYGFLEPLGYTLDVARNGNAGVACATGSC
                     HDAIVLDLSLPGLDGMEVCRTLRREYQLTTPILMLTARDTVHDKLAGFEAGADDYLVK
                     PYSLPELDARLKALVRRSRNEHVQAVLTFGELCLDAGIGRATRAGQPLDLTPTGYKIL
                     SALMRAAPRLMTREEIERQVWGDNPPESDALRTHIHTLRRTLDKPFPQPLLLTLPGTG
                     YRLATPDES"
     gene            6428..7687
                     /locus_tag="VFF22_18450"
     CDS             6428..7687
                     /locus_tag="VFF22_18450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZX18836.1"
                     /translation="MKARLSLKQQITLAYMLLAVTVASVFSLVSYISVAIIEDQVIDV
                     RLAKMADGLISAQQQGQLPDAPADVRFLIDASIPLELRQLPAGLHELTLDNQHVHAVL
                     REQGDSRYAVVQEVDELEHTELIILLSLASGFAVSVLLAIVLGMLSVRRLIAPVAALA
                     DAVQRNAGATELPSLNAQDEIGVLARAFAKRTDELQHFLQREQMFTGDVSHELRTPLT
                     VMLGAAEVLSAQLADQPLQYATAQRIRRVAEETAQRVSALLLLARAPETLDAPRTVLN
                     GIVEAEMERYRPLLQGKTVECRLEWHEQVAAHVRPELAGIIIGNLLRNACQHTEQGSI
                     TVRLAAGQLVVEDSGIGMPPRVRERLFERFVHDANWPAHEGTGLGLSIVKRVVDHIGW
                     DVRLETAQGGGTCFVLSFPALLATQTS"
     gene            7726..8481
                     /locus_tag="VFF22_18455"
     CDS             7726..8481
                     /locus_tag="VFF22_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HZX18837.1"
                     /translation="MSKVFKRKRMKRAVIAVGLLLSSGMAFCSDGPAWDYSGAKGPEN
                     WVKLSPEYGACAGSNQSPINLSGFIDAKLEPIAFAYNAGSAEILNNGHTVQVNVQAGS
                     SIVVDGIQFELKQFHFHVPSENLIKGKSFPMEGHLVHADKHGNLAVVAVMVTEGEASK
                     ALEKAWAQMPEKGEKLALSADISPMEILPENRDYYRFNGSLTTPPCSEGVRWLVMKQP
                     ITASKEQIEKFLAVVHHHNNRPVQAVNARPVLE"
     gene            complement(8545..8946)
                     /locus_tag="VFF22_18460"
     CDS             complement(8545..8946)
                     /locus_tag="VFF22_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZX18838.1"
                     /translation="MTILTISAACLLDDAGRLLLVRKRDTQAFMLPGGKAEPGEDALT
                     ALTRELQEELNLSLPANALQPLGQFRAAAANEADTWIDASVFQARLPHSVSAAAELEE
                     LRWLAQGDALPQTLAPLLREHVLPLLWPATQ"
     gene            complement(9010..9696)
                     /locus_tag="VFF22_18465"
     CDS             complement(9010..9696)
                     /locus_tag="VFF22_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017906909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX18839.1"
                     /translation="MFTLVHLDTPPPESLKSQILQMVVDYLSDISPVSLTPSNPLYQL
                     YQYVIGYEMHLYLQAMDSDRDTSAHLILALDDLDPSQVLGFALYLPAKDDPEACTLAY
                     MAVHTKQRRHGIARAMLQQMISRHPHAELACVASKVRTFEAMGFQVLAAQGPQVLMNT
                     RSHRSDGLVAVQDLAPIFHSKDVQQIHAYLVKQHGKRAMSDAEKQRDRLLDQMAQQAQ
                     ALVRERLIVH"
     gene            9875..10621
                     /locus_tag="VFF22_18470"
     CDS             9875..10621
                     /locus_tag="VFF22_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZX18840.1"
                     /translation="MSVNALYTDLSDYYDLMCADIDYQAQSHCIHRLQQLFGNAGSRH
                     LDLACGTGPHVRHFLDAGYTSSGLDINQPMLDKAALRCPQAHFALQDMCAFTVSEPLD
                     LITCFLYSIHYSGGIERLKACIASAHAALAVDGLFCFNVVDKGKIDNSLFAAHSAHHA
                     DGQFTFSSGWHYAGAGEQQVLRLQIEKTTAGETQVWHDEHPMVAVGFVELQALLQPYF
                     DVHVFEHDYQKLIPWDGTSGNALFACVKIG"
     gene            10726..11226
                     /locus_tag="VFF22_18475"
     CDS             10726..11226
                     /locus_tag="VFF22_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HZX18841.1"
                     /translation="MDLTDRILTTLLAEFSDLGQVENLTRATLRLSLAIFLGGLLGYE
                     REAKGKAAGVRTHMLVCLGASVFVMLLEMDGADHDAMSRVIQGVVAGIGFLCAGTILK
                     GEHIADVRGLTTAAGLWATAAIGVAVGLGHEATAVLSTLMALLILRVMPMLLDPKPDG
                     NEDKRE"
     gene            11388..11612
                     /locus_tag="VFF22_18480"
     CDS             11388..11612
                     /locus_tag="VFF22_18480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007348768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HZX18842.1"
                     /translation="MDLQKIEDEALHLPKKERAQLIQRLVLSLEAPSEEELRSDWLLE
                     ARRRAEELDSGSVQAVPGDEVMKKARALIR"
     gene            11609..11902
                     /locus_tag="VFF22_18485"
     CDS             11609..11902
                     /locus_tag="VFF22_18485"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZX18843.1"
                     /translation="MNYSFHPAAEAEFLESVGYYESKVRGLGGALITEFEALATLIGE
                     SPKAWRVELEPDIRRVPLHRFPLSILYRERPDGFQVLAVAHHRRRPQYWLGRL"
     gene            complement(11990..12691)
                     /locus_tag="VFF22_18490"
     CDS             complement(11990..12691)
                     /locus_tag="VFF22_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZX18844.1"
                     /translation="MPHILHLDASARPGLAGKDLHGSHSRNLTQRFVSQWLARRGQDS
                     LTYRDIGQNPPSIIDHDWIAAAFTAEEQREPWMQDALAESDQLVDELIAADVLVIGAP
                     LYNFSMPAALKAWIDQIVRAGRTVEVDESNPLDPYVPMLADRPRHAVILSARGGVGFG
                     AGGVMAHMNHLEPSLITALGFIGITQVHQIAIEGQEVGGDLLAESVAQAMNQVDALVT
                     QLQQALQVTRTPEPA"
     gene            12811..13710
                     /locus_tag="VFF22_18495"
     CDS             12811..13710
                     /locus_tag="VFF22_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZX18845.1"
                     /translation="MAQRWLPSLSALRAFEAAARHESAKQAAEELSVTATAVSHQIRA
                     LEESLGVALFLRKPRQLELTAQGRELQQVLESAFDSISAAATRLSALPCRQAITLSTT
                     PAVAVRWLLPWVCMLRDAHPTIDLRIHASHEPVALDGVTADIAIRYGDGRWPGLVAEK
                     LFDNSFIPACSPHLGLHDAAELPKHSLIHFRNQAAVSSPLDWAIWQKQAQVPGLDVGA
                     GLVFSDETHAISAAIGAQGVALMSRQLIEDELREGRLVQPFGPELEGKPFFLVYPENR
                     KHDPTVQAVRDWVMAVPGGLCTL"
     gene            complement(13824..14348)
                     /locus_tag="VFF22_18500"
     CDS             complement(13824..14348)
                     /locus_tag="VFF22_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18846.1"
                     /translation="MDTRFLTLMEFQSVYFYRFITLAIFVVIGVLLLFALPDMKRAEM
                     WVVVPVLFILWGRFAVEYFRVKKCGPAFIHNDELVITRDEGHLQIPLTRIRSVTSKHS
                     LFMVRRYRSWTDHLAFLQVTLNSGERVYTLAESKVFEYPAGKKTLSAIQAAVLAAKTK
                     SLTSQPNPDAERSA"
     gene            complement(14551..14859)
                     /locus_tag="VFF22_18505"
     CDS             complement(14551..14859)
                     /locus_tag="VFF22_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18847.1"
                     /translation="MNDSSNLILFLATQFLLPLGIAYAYCRSKGIATQSWRAGGIFLI
                     ALSVAMLFFTGLLGLPVHVKNVGFMTNRPMGLTCGAIGIVAGIVMGYVARRFESERSE
                     "
     gene            complement(15020..15556)
                     /locus_tag="VFF22_18510"
                     /pseudo
     CDS             complement(15020..15556)
                     /locus_tag="VFF22_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013790882.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            15788..16603
                     /locus_tag="VFF22_18515"
     CDS             15788..16603
                     /locus_tag="VFF22_18515"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZX18848.1"
                     /translation="MTTGRSTAYTAKAVRIEQIRVELNQRWLEQELERVNEVFLLALK
                     DAAEHCEQLRCELASQQLLEQEAKAKLTVVDKLRFKLGTPFSAASMSAAAQQLSIISE
                     RIETLQNDILLNSPGAKASSPNTSSKLLTAIAQNDKAVRLAKASNNSARSAWVEEQLQ
                     NQLLDERIQLDARRKEATQTKADRKALRSNVEMTVTIEGQRGMLTGVTRDISDTGVFV
                     VLKDADKTFTEGELLSVQVSGLRGESKVVSGVAVRVSGSGLGVRLNSAKDALK"
     gene            complement(17008..17754)
                     /locus_tag="VFF22_18520"
     CDS             complement(17008..17754)
                     /locus_tag="VFF22_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18849.1"
                     /translation="MEKIDKNTSNETIFVKIFWIAFAPLLGLLGYSAIYLTGVVYHQI
                     WVNHFSVGTGLFEKSTTDYFIYAYAALIQVCSNWLNVLTDPWIVLSTLGTVAFLVVEI
                     MLLAWLSRPNKKKTTNGAVSKNKKLDLLMTITALSTSITLILLALPVAANLFLITPAY
                     TGYKSAQLAIEKSTSVFSLGCEKVEKRQDYCVRLMDGDIEIAKGFVIDSSNERIALYL
                     DGKATILPLNDYRIETLIPPSKTTNTQAVR"
     gene            complement(18194..18442)
                     /locus_tag="VFF22_18525"
     CDS             complement(18194..18442)
                     /locus_tag="VFF22_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18850.1"
                     /translation="MFEFSIPDSIFNRKEIKATKDVKSFLAEVPHTPDEINEELAFEQ
                     MKLIYIRDIKLYRLKTGVNLALLWLVVGTTSFLISKLS"
     gene            complement(18747..19472)
                     /locus_tag="VFF22_18530"
     CDS             complement(18747..19472)
                     /locus_tag="VFF22_18530"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZX18851.1"
                     /translation="MEAKYSAYNHEESIKRIDQTLNNSDANYEDRKSIPPRNTLTFSN
                     GFYVDCAAIFVDMRDSKSLSEKHKRPTLAKIYKTYTSELVAILKDHSNVSEIYIEGDC
                     VWGVYDTPYKQDIDNLFSLAAKASSLIDTLNIKYKKKGYSEITVGIGLSYGSSLYIKA
                     GYSGSGVNEVIWLGKLVGEAAKLCSYGNKTYADKEVMVSDIFYDNLNESNKNLLSKNY
                     NRDCYHGNVVNIQMDEWVSKNAK"
     gene            complement(19605..20855)
                     /locus_tag="VFF22_18535"
     CDS             complement(19605..20855)
                     /locus_tag="VFF22_18535"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HZX18852.1"
                     /translation="MLSWDEFDKEDGADTAVASKASAQMADMNIDKLDQVGGAAAVEA
                     RAIAADDSAAVARAKAALDQLDIAEGLAELEGASARVAVDEKRMINCRADLNQLVPFK
                     YDWAWQKYLDGCANHWMPQEVNMNADIALWKTPDGLTDDERRIVMRNLGFFSTADSLV
                     ANNLVLAVYRLITNPECRQYILRQAFEEAIHTHAYQYCIESLGMDEGAIFNMYHEIPS
                     VAKKAAWGLKYTRSISDPTFTTGTVETDKELLRNLIAYYCVLEGIFFYCGFTQILSMG
                     RRNKMTGVAEQFQYILRDESMHLNFGIDVINQIKIENPHLWDAAMKDEATQMILEGTQ
                     LEIEYARDTMPRGVLGMNAAMMEDYLKFIANRRLTQIGLKEEYPGTSNPFPWMSEIMD
                     LKKEKNFFETRVIEYQTGGALSWD"
     gene            complement(21266..24175)
                     /locus_tag="VFF22_18540"
     CDS             complement(21266..24175)
                     /locus_tag="VFF22_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HZX18853.1"
                     /translation="MHTDTSRENPQANAPQATDDNQSLSATAPGQLRVIKRNGTVVPY
                     TDDKITVAITKAFLAVEGGTAAASSRIHETVERLTEQVSATFKRRMPSGGTIHIEEIQ
                     DQVELALMRSGEQKVARDYVIYRESRSQARKSGAATDIAEPHPSIRVTHLDGTVAPLD
                     MGRLNTIVTEACEGLAEVDGALIQKETLKNLYDGVAQSDVSTALVMTARTLVEREPNY
                     SYVTARLLMDNIRAEGLGFLGVATSATHHEMSELYAKALPAYIAKGIEYELLNPVLAT
                     FDLEKLGKAINHERDQQFTYLGLQTLYDRYFIHKDGTRFELPQVFFMRVAMGLAIEEK
                     QREDRAIEFYNLLSSFDYMASTPTLFNAGTLRPQLSSCYLTTVPDDLSGIYNAIHDNA
                     MLSKFAGGLGNDWTPVRALGAYIKGTNGKSQGVVPFLKVVNDTAVAVNQGGKRKGAVC
                     AYLETWHMDIEEFIELRKNTGDDRRRTHDMNTANWIPDLFMKRVFDDGQWTLFSPSEV
                     PDLHDLTGKAFEERYEYYEALTQFGGKIKLFKTIQAKDLWRKMLSMLFETGHPWLTFK
                     DPCNLRSPQQHVGVVHSSNLCTEITLNTNADEIAVCNLGSVNLPNHIVDGKLDTAKLQ
                     KTIKVAVRMLDNVIDINYYSVPQAKNSNFRHRPVGLGIMGFQDALYLQHIPYGSDAAV
                     EFADKSMEAVSYYAIQASCDLADERGSYETFQGSLWSKGILPLDSQQILIEQRGQKYI
                     DVDLTESLDWAPVRARVQKGIRNSNIMAIAPTATIANITGVSQSIEPTYQNLYVKSNL
                     SGEFTVINPYLVRDLKARGLWDSVMINDLKYYDGSVQQIERIPQELKDLYATAFEVDT
                     KWIVDAASRRQKWIDQAQSLNLYIAGASGKKLDVTYRMAWYRGLKTTYYLRALAATST
                     EKSTINTGKLNAVSSGGNDGLSAAPAKAPEVEMSAGPAPVPKACAIDEPDCEACQ"
     gene            24894..25616
                     /locus_tag="VFF22_18545"
     CDS             24894..25616
                     /locus_tag="VFF22_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX18854.1"
                     /translation="MEQQSWHILIVEDDQRLAELTREYLQSNGLNVSIEMDGAQAAAR
                     ILQEQPDLVILDLMLPGEDGLSICRKVRGQYEGPILMLTARTDDMDQVLGLEMGADDY
                     VCKPVRPRVLLARIRALLRRHEGAEVQAPSERRRLQFGPLVIDSAMREAWLGEQSIEL
                     TSAEFDLLWLLTSNAGRILSREEIFNALRGIEYDGQDRSIDVRISRIRPKIGDDPMHP
                     RLIKTVRSKGYLFVAEAAEALT"
     gene            25627..>26781
                     /locus_tag="VFF22_18550"
     CDS             25627..>26781
                     /locus_tag="VFF22_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003214234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HZX18855.1"
                     /translation="MNSIFLRIYGGMLAVLVLVALLGVGALQLLNDVRVDQYRERLAQ
                     GTFRLMADNLSPMVDIERRRAVTVWGRLLGIPMRVETLESAGLDSGARNSLRRGQVLV
                     QQTGPHAARIFSQLDEQPLLLIADVQQVSEQLARATSFLLIDELVRYPWDEQPRRLAA
                     LKQDKQFGFDLRLVRLDQADLDPDQRRRVDEGDTVMALGRGGDSIHVFSGIVETPWVL
                     EIGPLYQMNPYPAHWLVLIGALGLSLIGLMVYLLVRPLEQRLSGLEGAATRIASGRLD
                     ARVPTRGSDSVGRLAAAFNAMAEHLQRSLSIQREMVRAVSHELRTPVARLRFGLEMIG
                     DAESEAARRKYMDGMDSDIQDLDKLVDEMLTYARLEQGAPALNFQSVDLNE"
BASE COUNT         5111 a         7359 c         8295 g         6016 t
ORIGIN      
        1 gggcctgcaa cctatttccg tcacccacaa ggccgagtca ctggaacagg ccatcgacgg
       61 ctccatcgaa aaactgcacc acgcccttga gcatcaattc ggcaaactgc gcagcaagcg
      121 cgtcacgcca ctgcacgccg acgaggcgaa cgatattccc ggccaagacg cactgctgga
      181 ggaggacttc ctcgccgagg agcagctgcg cgccgttaac tgaataccac gggcagcggg
      241 ccaggctggc tgcccatcaa tcccttgctc gcgcccccgc aggtttctgc gggggcgttg
      301 ttgtttcagg gtttgcccat agcggcaaat gcctgcctgg agcggcagag cgcttcccct
      361 tcgctcaagg ctgaaacggc gcatagccct ggttttactg ggctataggt ggttggccca
      421 tattctgcag aaatgcctgc cagctgacct acgcagggat tcgccatgac cagtatctcc
      481 aaccactcac cgctggcgag ctacttcgcc aacaccgcca aacccagcac cgctggggct
      541 gcaggcagtg gcagcagtac ggacaaacca aacaccagtg cgctagaccc gcttgcggaa
      601 atccgccgct ttgccagcgg catggtggct aacagccgtg gtggtctgct gcgcgcgatg
      661 cacgccgaga gcagcggtgc gagcaacgcg attgccagtg atcgctttcg cccggtggcc
      721 gagccagaga gcgacatctc gaaaattgtc ttgccggatg tcgccgaact ggaccgtgaa
      781 gacgccgcca agctgctggc gcagatcaac aaggtggtcg atgccggcct ggacaagacc
      841 gacagctttg tcggctacaa cggcgaccag cagaccgact cgctgaccac ctaccgagat
      901 tggctgcagg ccaggggcgg cgtcagcatt tacgtataag cgctcggcca ccgaattcgc
      961 cagtcgcgct gcctgcgcat tgggctggtt tcagcgcgcg aggtgcgcca agatcacccg
     1021 accacagtga tgggcacacc gcatgctcag gtcgagggga tttatggcaa gcaaggaatt
     1081 tattcgggcg ttcgccgccc tgggattggc gctgttgtgc gccactggtg cgcaggccgc
     1141 cgaggatgcg caactgatcg ggctgatcaa cagctaccgc agcgaagtac agcgttgtgg
     1201 cgatcaggcc tccgccgagt tgccgccgct gacggccgat ccacgcctgg tgcagccggt
     1261 gggccgctcg ggggatttgc agcaggccat gagcgcggcg ggctacccga tggtcaacgt
     1321 gcaggccatc agcctgtccg gcccgcgcga tgcggccgcg gcgctgaagg tgctgcagga
     1381 gagcttctgc aagattgtgc tggacccgca gtttgtcgat atcggcgtta atcaacagga
     1441 ccgcgagtgg cgcatcctcc tggccaggcc attgctggcg gcgcgcctgg gcgattggca
     1501 ggcggaaggg cagaggctgc tcgaacaact caacagcgcg cgcggtacgg cacgccagtg
     1561 cggcggccaa gcctttgccg ccgccgcacc gctggtgtgg aatgccaccc tgggtagcac
     1621 cgccgaagcc cacagtcggg ccatggctaa cggcaacttc ttcgatcacc aggaccctga
     1681 cggccgcacc cccggcgacc gtgcagaatt ggccggctac agcggccagc gcgttggcga
     1741 aaatatcgcc gccgggctgg ataacgccag caaagtgctc gctggctggc tggccagccc
     1801 cggccactgc gccaacctga tgaacccgca attcagcgag ctgggcgccg cctatgcaaa
     1861 cgatccgaag agcgatgcgg ggatttactg gacggcgctg tttggcgcgc cgtaagggag
     1921 gtaatgccga ccataattcg ttatggatcg gcgggtgaac tctggttctt gcagtggtgc
     1981 gtgcagccag gctcatcaac tgctggaact tcgggcgctg cagacactgc ggctgtttac
     2041 agcttgaagt gcccgaccag ctcgtccagt cgtgtcgaca ggtcctgcaa gctggtgctg
     2101 gattggctga gctggtcggc gatttgggtg gtttcctggg ttaggttgtt gatttcttcg
     2161 acgttgcggt tgatttcctc gaccacgcag gactgttctt ctgtggccgt ggcgacctga
     2221 atgttctgat cgctgatctg cgtgatatgg ccgttgatct gctccagggc ggccgaggcc
     2281 ttgccggcaa actccactac tcgttcgctc tgctgacgtc cgcgggacat gacatccacc
     2341 gctgcgcggg attcggcctg cagacggttg atcacttgct gaatttcttc ggtcgatgcg
     2401 cccgaacggc tggcgagggt gcgaacctcg tcggccacca cggcgaaccc acgaccttgc
     2461 tcgccagcac gggcggcttc gatggcggcg ttgagagcca gcaggttagt ctgctcggag
     2521 atgctgcgaa tggtgttgag tgtcgagctg atcgcagtga tctgtcctgc cagtgcctcg
     2581 accacgttgg aggcttgctc cagttcgcca ctgagtgtgt tgatctgctg gtgtgcttgg
     2641 gccactacgt cgcggccgtc gctggcgtgc cggctggctt cgtgggccac atgggcagca
     2701 ttttcagcat tgccggcaat ttcattgacg gtcgaaccaa gctcatggat ggctgtggcg
     2761 acctgcaccg tgcggtcgct ctgcgaggtg cagttgctct gcgtcaggcg cgcgcgctcg
     2821 gccacttcac gggccatcgc ggacaactga cgtgagttgc ctgccaactg ctccatggtg
     2881 ctgtggacct tggcgataaa gcggttgaag ctgttggcaa tctggctcag ttcgtcttcg
     2941 ccgggcagtt cgatacgggt gttcagagac aggttctctg cggcattgcc cagcttgtcc
     3001 tgcaagatgg agacgcgccg acgcaggttg ctgacgatca accaggccgt gataaaggat
     3061 gccaacagac caagggtgat gatgatcaac tgacgactgc ggccgttgac atagctttca
     3121 ttgctgagct ggttctgttg ctcggcctgt tgcagcagac cgtcgagaag ctcgttggca
     3181 cccaggcgca taaccccata gcttttggcg tattgatcgc ggtagatctg ctgggcgcgg
     3241 cccatgtcgc cggcgtcgag ggcctgtagc agcggcgtca gctcattgct gaccatactt
     3301 tcaaattgtt gcagcaggcg ctctgcctgc gcctttcgat ccgggtttac ctgggcgtcg
     3361 accgcgtggc gcatggcctc gcgcatgccg ggaatatcct cattgagcgc ttcttgcacc
     3421 cgcgttttga ctccgcgctc gtcgcgtagg cctgtgtcct gcagcagcat catgtcgata
     3481 cccacgcgca tgcgcggaat gcgcgaggcc gtctctgcca tggcgcgcat cggcgccgag
     3541 gtattgaggt acagcgcgct ggcttgctgc tgcatggtgg acatgctgct caagctggta
     3601 accgccacca aaattagggc aatgcaagga atggctactg cgattatcag gcgggtttta
     3661 agagtcagag agtcaaggcg cataaggctc attctttatt aggaggaaac gacgttacca
     3721 agcagcgttt tttgtaagtg tcggcatggc tgcctcagca tagatgcttg tgcaggtgat
     3781 cggcaggacg aggccgatca tgagaccttt gcttcattgc agctgctgta tattgcgtta
     3841 acgtcgtccc cacggtttcg ataatgacca gaccccactg cgtaggccac gccatgcata
     3901 tcaggttgta ctgatctgac caccaagccc tgggtgcagg aagctggcat tgaggtgctg
     3961 gaacaaccct tcttcgctcg cggtaacttg gccactgccg gcggttgcct ggcctcgatc
     4021 tatctcgctg cctggaccct ggcgcgcctg gtcgggcgtg atgcggcggc cgaggcgctg
     4081 cactatgtgg cgccggttgg ggagaaagag gcgtatgtgg cgcgtgcgct cggcaatatc
     4141 gaggcctatc tctaggcgcg gcttatggcc gtgaaaggcg gctatccggc gcgagaaccg
     4201 ccaaggctta tttcagcggt ttctccacca ctacataacg cttgccacca ctgcgtttgg
     4261 ccacgtacat ggcttcatcc gcctgctgca ccagctgcag ttcgctctgg ccgtgatcgg
     4321 ggtgcactgc gatgcccaga cttggtgcgc tgatcaagtg gtgcccatcc agcaggtaag
     4381 gcgcgctgag ggcagcaata atcttctcgg ccaccagcgt ggcatcctcc tggcgaccga
     4441 tgttgtcgac gatcacggcg aactcgtcgc cgcccaaacg gcccaccgta tcggattcgc
     4501 gtacgcactg gagtaggcgc cgcgcgacct cttgcagcaa caggtcgcct gcgctgtggc
     4561 cgtaggtgtc gttgatctgt ttgaagttat ccatgtcgag atacagcacc gccaggcgca
     4621 ggctgtcgcg tttggcccgc tgcaatgcac tgcgtaagcg gtcgagaaac agcgtgcggt
     4681 tggacagctc ggtcagctgg tcatattggg cgagaaactc caggcgcgag tgcgtgcgct
     4741 tgcgctctat cgccgccgcc acctggttcg atacaaactg cagcagctcc tggtcctgtt
     4801 cgttgtaacg cacgctggcg ctgtggttct gcagcaccag tgcaccgagg ggctgggtgc
     4861 ctgaaattag cggcacgccc agccagtcag catgcgtagc cgtcgcagtg ccctgcgggg
     4921 ctgtttcagg ggttaacagc agggcgcgcc ccgcgcgaat gacctcggcg cagaaatcac
     4981 gggtggctat tggtgagtca tccggcgcga gcgggtaggc aacgctgagc tggtcactcg
     5041 cagcatcgtg cagcgctacg cagaaatgtt gggcgggcag taattgcgcg accacctcat
     5101 gcacctgctc gtaaagcctg tgcaggtcgc caaccgcgtg ggccgcttcc gataccgcat
     5161 acaacgccgc ctgcaccgcc tcagcccgct tgcgccgggt gatatcgcgc gccacgccaa
     5221 cccgcacctg ctcggcctca agccactggg cagaccacat gatgtgcacg atatggccgt
     5281 ctttatggat atagcggttt tcatggtctg acgagggttg gcctgaattg atgcttttta
     5341 cgagtgcccg gctggcggcg cgatcgtccg ggtgcatcaa ttcaaaggcg ctgcggccaa
     5401 tcatttcttc gggccgataa ccgaaaaccc gctcactggc cgcgctgacg gagagaaagc
     5461 gaccgtcctt gtccactacg cagacaacgt ccagcagcag atccagtagc ttgtcgtggg
     5521 gcaggtgtgg gtttgacgtc atggcggtga atatactgtg cgacccgcag gcttcgctag
     5581 cgtgatgatt tttcgctaaa cgtgactcgc cgcaaggccg aagttcgtaa tagcggttgt
     5641 agttgggtca tgccggatga gttgctggcc gccgctgaaa ggggtgccaa tcagcaaggt
     5701 gatcacccag gtagtttttc aacagtccgc caacgaccga aaagtggggc tcttatgcat
     5761 gtactgattg tcgaggacaa cccggacatc atcgccaacc tctacggctt tctggagcct
     5821 cttggttata cgttggatgt ggcgcgcaat ggcaatgccg gtgtcgcgtg cgctacgggc
     5881 agttgccatg atgcgattgt ccttgatctg tcgttgcccg ggttggatgg catggaggtg
     5941 tgccgcacgt tgcgccggga gtatcagctg accacaccaa tcctgatgct gaccgcgcgg
     6001 gacacagtgc acgacaaact ggctggtttc gaagccgggg ccgacgatta cctggttaaa
     6061 ccctattccc tgcctgaact ggatgcccgc ttgaaggcgc tggtacgtcg ctcacgcaat
     6121 gagcatgtgc aagcggtgct gacctttggc gagctgtgcc tggatgcagg tatcggccgt
     6181 gccacccgcg cagggcagcc gctggacctg acacccacgg gttacaaaat tctcagcgca
     6241 ctgatgcgcg ccgcgccaag gctaatgacg cgtgaagaga tagagcgcca agtgtggggc
     6301 gacaacccgc cggaaagcga tgccttgcgc acccatatcc ataccctgcg ccgcacgctc
     6361 gataaacctt tccctcagcc tctgctgctg acgctgccgg gcactggtta tcgactggcg
     6421 actcctgatg aaagctaggc tctcgcttaa gcagcagatc actctcgcct acatgctcct
     6481 ggcggtgacg gtggccagtg tctttagcct ggtttcctat atttccgtgg cgattatcga
     6541 ggatcaggtc atcgacgtac gcctggccaa gatggccgac ggactgatca gcgcccagca
     6601 gcaggggcaa ctgccggatg cgccggcgga cgtacgcttc ctcatcgacg ccagtattcc
     6661 gctggagttg cggcagttgc ctgcgggcct ccacgaattg acgctcgaca accagcatgt
     6721 ccatgcagtg ctgcgtgagc agggcgacag ccgctatgcg gtggtgcagg aggttgatga
     6781 gctcgaacat acggagctga tcatccttct gtcgctggcc agcggctttg ccgtcagtgt
     6841 gctgcttgcc attgtgctgg gcatgctcag tgtgcggcgg ctgatcgcgc ccgtggcggc
     6901 actggctgat gcggtgcagc gcaatgccgg ggctacggaa ctgccgtcgc ttaacgcgca
     6961 agatgaaatt ggcgttctgg cccgtgcttt cgccaagcgg accgacgagt tgcagcattt
     7021 cctgcagcgc gagcagatgt tcaccggcga cgtcagccat gagctgcgca cgcccttgac
     7081 cgtcatgctg ggcgcggcgg aagttctgag cgcacagctg gcggaccagc cgctgcagta
     7141 cgccacggcg cagcgcatcc gccgggtggc cgaggaaacc gcgcagcggg tcagtgcgct
     7201 gcttctgctt gcgcgcgcac cagagacgct ggatgcccca cgtaccgtgc taaacggcat
     7261 tgtcgaggct gaaatggagc gctaccggcc gcttttgcaa ggtaagacgg tcgaatgccg
     7321 gctggagtgg cacgaacagg tggccgccca tgtgcgcccc gagctggcgg gaatcatcat
     7381 cggtaacctg ctgcgcaacg cctgccaaca taccgagcag ggcagcatta ccgtgcgact
     7441 tgctgctggc caactggtgg tcgaggacag cggtatcggc atgcccccgc gggtgcgcga
     7501 gcgtttgttc gagcgcttcg tccacgacgc taattggccc gcgcatgagg gcacgggcct
     7561 ggggctgtcg attgtcaaac gtgtggttga tcatatcggc tgggacgtgc gcctggagac
     7621 tgcgcaaggc ggggggacgt gtttcgtcct gtcatttcca gcgctgctcg ccactcaaac
     7681 ctcttaacgc cgccttaacg ccggggtgtc tatcgtgtgg cttccgtgag caaggtattc
     7741 aagaggaagc gcatgaaaag agcagtgatt gcagtgggtc tgttgttgtc atccggcatg
     7801 gcgttttgca gtgacggtcc tgcctgggac tacagcggtg ccaaggggcc ggagaactgg
     7861 gtcaagctca gcccagagta cggcgcgtgt gccgggagca atcagtcacc gatcaacctg
     7921 agcgggttta tcgatgccaa gttggagccc atcgcctttg cctataacgc cggcagtgca
     7981 gaaattctta acaacggcca caccgttcaa gtaaacgtcc aggcggggag ctccatcgtc
     8041 gtggacggta ttcagttcga gctaaaacag ttccactttc acgtgcccag tgaaaacctg
     8101 atcaaaggca agtccttccc catggaaggg cacctggtcc acgccgacaa gcatggcaac
     8161 ctggccgttg tagcggtcat ggtgaccgag ggcgaagcca gcaaggcgct ggaaaaagcc
     8221 tgggcgcaga tgccggaaaa aggcgaaaaa ctggcgctga gcgcggatat ctccccgatg
     8281 gaaatcctgc ctgaaaatcg cgattactac cgcttcaatg gttcattgac cacaccccct
     8341 tgctctgagg gcgtgcgttg gctggttatg aagcagccga ttacggcctc taaagagcaa
     8401 attgagaagt tcttggcggt cgtgcatcac cacaacaatc gcccggtgca agcggtcaac
     8461 gcccgcccgg tattggaata agcgatacag tgactgcgag tctggccggt tgttgagaac
     8521 tgcctggtta aacccgtctt gagtttactg agtcgctggc catagcaacg gcagcacatg
     8581 ctcacgcagc aacggtgcca gtgtctgcgg caatgcatcg ccttgtgcga gccagcgcag
     8641 ttcttccagt tcggcggcgg cgctgacgct gtgcggcaag cgggcctgaa acacgctggc
     8701 gtcgatccag gtatcggcct cgttggccgc agctgcgcgg aactggccga gcggttgcag
     8761 tgcgttggcc ggtaggctga ggttgagttc ttcctgcagt tctcgggtca gggcggttag
     8821 agcgtcttca ccgggctcgg ctttgccgcc gggcagcata aaggcctggg tgtcgcgttt
     8881 acgcaccagc agcaggcgac cggcatcgtc gagcaggcag gcggcggaaa tagtcagaat
     8941 ggtcattggc gtttcgcttg tgtgtggcgc gctccgcgcg gtttctctgt tgtggctgtc
     9001 atgctgcgct caatgcacta tcagccgttc gcgtaccagc gcctgcgctt gctgggccat
     9061 ctgatcgagc aggcggtcgc gctgtttttc cgcatcgctc atggctcgct tgccgtgctg
     9121 ctttaccagg taggcgtgga tttgctgcac gtccttggag tggaagattg gcgccaggtc
     9181 ctgcaccgcc accagaccat ccgagcgatg actacgggta ttcatcagca cctgcggccc
     9241 ctgcgcggcc agcacctgaa agcccatggc ctcgaaggtc ctcactttgc tggcaacgca
     9301 ggccagttcg gcgtgcgggt ggcggctgat catctgttgc agcatggcgc gagctatccc
     9361 gtgccgacgc tgcttggtgt gtacggccat ataggccagg gtgcaggctt cggggtcatc
     9421 cttggcgggc aggtacaggg caaaaccgag cacctgggac gggtcgagat catccagggc
     9481 cagaataagg tgagcgctgg tatcccgatc actgtccatg gcctgcaggt acaggtgcat
     9541 ctcgtagcca atcacgtact gatacagctg ataaagcggg ttactgggcg tcagcgacac
     9601 cgggctgata tcgctgaggt agtccaccac catctgcaga atctggcttt tcagtgactc
     9661 gggtggcggg gtgtccaggt gtacaagggt gaacatcgtc agtcgggctc cgggcgtgcg
     9721 cgattgggcg gtcggtgtgg gctgcattgt aacgccctcg gcttggcggg tttgcatcgg
     9781 cttgctgctc cggtcagcag gatctgaacg gggttggcta ggcttgggct atgatgcccg
     9841 cccgcccgcg ccgctcgccc atcgtcaggt cactatgtcc gttaatgccc tctataccga
     9901 cctgtctgat tattacgatc tgatgtgcgc ggatatcgac taccaggcgc aaagccactg
     9961 catccaccgg ctgcagcagc tgtttggcaa cgccggcagc cgtcacctcg atctggcctg
    10021 tggaaccggg ccgcatgtgc gccattttct cgatgccggc tataccagta gcggcctcga
    10081 cattaaccag ccgatgctgg acaaggccgc gctgcgctgc ccgcaggcgc acttcgcttt
    10141 gcaggatatg tgcgctttta cggtgagcga gccactggac ctgatcacct gctttctcta
    10201 ttccattcat tacagcggcg gtattgagcg gctgaaagcc tgcatcgcca gcgcccatgc
    10261 agccctcgca gtcgatgggc tgttctgctt taacgtggtc gacaagggca aaatcgataa
    10321 cagcctattt gccgcgcaca gcgcgcacca tgctgacggc cagttcacct tcagttccgg
    10381 ctggcattac gccggtgcgg gtgagcagca ggtattgcgg ctgcagattg aaaagaccac
    10441 ggccggtgag acccaggtgt ggcacgacga gcacccgatg gtggcggtgg gctttgttga
    10501 gttgcaggcg ctgttacagc cgtattttga tgtccatgtg tttgagcatg actaccagaa
    10561 gcttatcccg tgggatggta cttccgggaa tgcgctattt gcctgcgtaa agattggctg
    10621 atcgttgctg cgtttcgcgg gcaaccctgg gctagacgct ccgcacgcct gcatatatgt
    10681 cgtcgctgcg tactcagaga ttaatcgccc taacaggagc aactgatgga cctgaccgac
    10741 cgtattctga caaccctgct ggccgaattt tccgaccttg gccaagtaga aaacctcact
    10801 cgcgccaccc tgcgcctttc cctggccata tttttgggcg ggttgcttgg ttatgagcgc
    10861 gaggcaaagg gcaaggctgc gggtgtgcgt acgcatatgc tggtgtgtct gggggccagt
    10921 gtctttgtca tgttgctgga aatggacggc gcggatcacg atgccatgag ccgggtgatt
    10981 caaggcgtcg ttgcgggtat cggctttcta tgcgcgggca ccattctcaa aggcgagcac
    11041 attgccgatg tgaggggcct gaccactgcc gccgggctat gggccactgc ggccataggt
    11101 gtggctgtgg ggctgggcca tgaggcgact gctgtgctca gcacgctaat ggcgctgctg
    11161 atactgcgtg ttatgcctat gcttttggac cctaagcccg atggcaatga ggacaagagg
    11221 gaataggcga ggctggtttg agtacatacg ccgcttatgt gttgttttcg ggcaatggtt
    11281 cccctttatc cagctgggtt agcccttgta aagcccagat atccggccat ggcacccgct
    11341 agccatccgt gccaagattt gccggtagat aagcgagagg ttttgtcatg gatctgcaaa
    11401 aaattgagga tgaagccctg caccttccca agaaggagcg ggcacagctg atccagcgtt
    11461 tggtcctgag tctggaagct ccctcagaag aagagcttcg gtcagactgg ttgcttgagg
    11521 cacggcggcg agcagaagag cttgatagtg gctcggttca ggctgtgccc ggtgatgagg
    11581 tgatgaagaa ggctagagcc ctgattcgat gaattattct tttcacccgg cagcggaagc
    11641 cgagtttctg gaatctgtcg gttattacga atcgaaggtt cgcggattgg gtggtgccct
    11701 cattacggaa ttcgaggccc tggccacatt gattggcgag tcaccaaaag cctggcgagt
    11761 tgagcttgag ccagatattc gcagagtccc tctccaccgt tttccgttat ctatccttta
    11821 ccgggaaagg cctgacggct ttcaggttct ggctgttgcc catcaccgcc gacgtccgca
    11881 atactggctt ggcaggcttt aactccccta gcgtttttga ccatctaggt ggacaagcgg
    11941 cgcgttgtcc accctacaca gcagtcaccg tggcgcaggc cggcggtttt caggccggct
    12001 ctggcgtgcg ggttacctgc agcgcctgct gcagctgcgt taccaaggca tcgacctggt
    12061 tcatggcttg agccacggac tcggccagca gatcaccgcc gacttcctgg ccctcgatgg
    12121 caatctggtg cacctgggta atgccgataa agccgagggc ggtgatcagg ctgggctcca
    12181 gatggttcat atgcgccatc acgccgccgg cgccaaagcc gactccacct cgggccgaga
    12241 ggatgacggc atggcgaggg cggtcggcga gcatcggcac gtaaggatcg agtggattgc
    12301 tttcgtcgac ctctacagtt ctgccggcac gcacgatctg gtcgatccag gccttgagcg
    12361 cagccggcat gctgaagttg tagagcggcg cgccgatcac cagcacgtct gcggcaatca
    12421 attcatcgac cagttggtcg ctctccgcca gcgcgtcctg catccacggc tcgcgctgtt
    12481 cctctgcagt aaaggcggcg gcgatccagt cgtggtcgat gatcgacggc gggttctggc
    12541 ctatatcgcg ataggtgagg ctgtcttggc cacggcgagc tagccattgg ctgacaaaac
    12601 gctgagtcag gttgcgactg tgtgaaccgt gcagatcctt gccggcgagc ccggggcggg
    12661 cgctggcgtc caggtgcagg atatgtggca tgtcgagtct ccttttcgaa gttgaattca
    12721 tctgttattc actgcaagtt caggctaagt tcagtccgca cgggcgacaa acgacgatta
    12781 ttttcatgta tggatgagga gagttcatgt atggcgcagc gctggttacc ttcgctttct
    12841 gccctgcggg cctttgaggc ggccgctcgc cacgagagcg ccaagcaggc cgcagaagag
    12901 ctttcggtga ccgcgacggc ggtcagtcat cagattcgtg ccctggaaga gtcgctcggc
    12961 gttgcgctgt ttctgcgcaa gccgcgccaa ctggaattga ccgcccaggg gcgtgagctg
    13021 cagcaggtgc tggaaagcgc cttcgacagc atcagcgccg cggctacacg cctcagtgcg
    13081 ttgccctgtc gccaggccat tacgctcagc accacgcctg ccgtggcggt gcgttggctg
    13141 ctgccctggg tgtgcatgct gcgcgatgcc catccgacca tcgacctgcg catccacgcc
    13201 tcccatgagc cggtcgcgct ggatggcgtc accgcagata tcgccatccg ttatggcgac
    13261 ggccgctggc cggggctggt ggctgagaag ctgttcgaca acagttttat cccggcctgc
    13321 agcccacacc tcggtttgca tgatgcggcc gagctgccca agcattcact gattcacttt
    13381 cgcaatcagg ccgcagtttc ctcaccgctg gattgggcta tatggcagaa gcaggcccag
    13441 gtgccggggc tggatgtcgg cgccgggctg gtgttctccg acgagaccca tgccatctcg
    13501 gccgccattg gcgcgcaggg tgtggcgctg atgagccggc aactgatcga ggatgagctg
    13561 cgcgaagggc ggctggtaca gcccttcggt ccggagctgg aaggcaaacc gttctttctg
    13621 gtctacccgg aaaaccgcaa gcacgacccg accgttcagg cggttcgtga ttgggtcatg
    13681 gccgtgccgg gtgggctgtg tacgctgtag gttggccgat atagctgggt tgacccggtt
    13741 cctgttgcct gtgcgatgtg aagtcaggct gccttactgg gcggtgccgt gcgcttggtg
    13801 cgctactccc gcatttcgcg cacctaagcc gaacgttccg catcagggtt tggctgtgat
    13861 gtgaggctct tggtcttggc cgcaagcacg gcagcctgaa tcgcgctcag ggttttcttg
    13921 cccgctgggt attcaaacac tttgctctca gccagggtgt aaacgcgctc gccgctattc
    13981 agcgtgacct gcagaaacgc caggtgatcc gtccacgagc gataacggcg caccataaac
    14041 aggctgtgtt tggaagtgac cgagcgaatg cgggtcaagg gtatctgtag gtgaccctcg
    14101 tcgcgggtaa tcaccagttc atcgttatgg ataaacgcag ggccacattt tttgaccctg
    14161 aaatattcaa cggcaaagcg gccccacagg ataaacagca ccggtacaac cacccacatt
    14221 tcggcgcgtt tcatatcggg caatgcgaac aacaacagaa cgccgattac cacgaaaatg
    14281 gcgagcgtaa tgaagcggta gaaatagacg ctttggaatt ccataagggt tagaaaacgg
    14341 gtgtccatac gcgtgttatc cctcgacgtt gtgtaattga gcgctgatgg gctatccggc
    14401 atttgagcag agtagggtgc tggctgcgct agccaagctt ggaaaatgtg ctctgccccc
    14461 tgcttcgcat gggtgctttc tcgactgacg tttggccgca gcttttgcat cggctatatg
    14521 acggtgtcgc gcgcccctgt tgcatctggg ctactcgcta cgctcgctct cgaaacgtcg
    14581 cgctacatag cccatcacga taccggcgac gataccgatg gctccgcagg tcaaccccat
    14641 gggcctgttt gtcataaagc cgacgttttt cacatggact ggtaagccga gcagcccggt
    14701 aaaaaacagc atcgcaaccg acagggcaat gagaaatatg ccgcccgctc gccacgactg
    14761 ggtggcaatg cccttgcttc ggcaataggc ataggcgatg cccaacggca gcaaaaactg
    14821 ggtggcgagg aataggatca gattgctgct gtcgttcatc gtcgtctgct tctgtgtggg
    14881 ccctagcagg gtattgcatc tgcctgacat gccgggtgat ctgtcacgca aaacagtagt
    14941 gagagtaggc ggcaagtcag tgacgctaag acgatcattc ctaggctctg tgtgggaata
    15001 cagccggtga cgctctgcgt cacgagtccc agcggtctac ctcgatcaac cccgcctggc
    15061 ctgcataatt cctggcactg gagtaacgcc aatgctccgc tcggtctaca tagccacgct
    15121 tgagtggatt gtgatggatg tagtcgagtt tctctctcat cacctcattg ctctacacca
    15181 gctctgcgtg tgacccctcc tgccagagtt gatacacgcg atcatgcttg tgtgcacgct
    15241 tggcgcagcg cagccgcgcc agcaaacgct ccgcccctcg cgtttgcagg tgatcgatga
    15301 tttgccgtgc cgtgaaggat ttgaacccgc tgacacattt gctcaggtca ggtgcttggg
    15361 caacgaagtg caggtggttt tccagaacga catagccata gaggcgtaag ccctggtttg
    15421 cctgttgata gcgccagcaa tcgagcagga tgtcgaccag cgccggtcga gtaaacagcg
    15481 gtagccattc cagcaccgtg caggtgagaa agtgtggttt ctcgggttcg gtgatgaggt
    15541 agcggctacg gcccatggat tcctccttga atcgattggt aggtatctga tcgtacccag
    15601 gctccgcgtg ggccgcattt gaggacgctc cgcgtcctgc tttctgttac gtgattgaga
    15661 ttagctgagg tgtagagcct ggtagtgatc gacgcagagc gtcgcggatt gtattcccac
    15721 gcagagcgtg gggacgatca aaaagatata ctcgaaaaac atggactgaa tgtatggtgt
    15781 tgaatctatg acgaccggcc gctccacagc ctacacagca aaagctgtgc ggattgaaca
    15841 gatcagagtc gaactgaatc agcgctggct tgagcaggag ctcgagcgcg ttaacgaggt
    15901 tttcctctta gcactcaaag acgctgcgga gcactgtgaa caactgcgtt gtgagcttgc
    15961 gagccagcaa ctgctggagc aagaagccaa agccaaactg accgtcgttg acaagcttcg
    16021 ctttaaactt gggactcctt tttcggctgc aagtatgtcg gcagcagcgc aacaactatc
    16081 gataattagt gagcgtatcg agacgctgca aaatgacatt ctactgaaca gcccaggagc
    16141 taaagcctcc tcacctaaca ccagttcaaa gttgcttaca gcaatagcgc aaaacgataa
    16201 agcagtacgc ttagccaaag ccagcaataa cagcgcgcga tccgcctggg tagaagagca
    16261 gctgcaaaac caactgcttg atgaacgaat ccagttagac gcacggcgta aagaggccac
    16321 tcaaaccaaa gccgaccgca aagcattgcg ctctaacgtt gaaatgacag tcaccatcga
    16381 aggccagcgc ggcatgttga cgggcgtgac tagagacatc tccgacacgg gtgtgttcgt
    16441 ggtgttaaaa gatgccgata aaaccttcac cgaaggtgag ttgctttctg tgcaggtcag
    16501 cggcctacgt ggtgaatcaa aggttgtaag cggggttgcg gtgcgcgtta gcggcagtgg
    16561 tttgggcgtg cgcttgaact ccgctaagga tgctctgaag tagccctata tttccggccg
    16621 ccttcagccc ttgctgattt ttaaaacagc tatttaccgc accgatagct cccgcgtggg
    16681 acgatcaagt accacccagg cattgatcgt acccacgctc cgcgcgggta cgcatttgag
    16741 gacgctccgc gtcccgcttt tgttccgtgt tagagactag ttgaggtgta gagcctggta
    16801 gtgatcgacg cagagcgtcg agggttgcat tcccacgcag agcgtgggaa cgatcaaatt
    16861 gttctggccc atcgcggctc tcgcggcatc catgccgctc aaccccttgc gcaacaattc
    16921 cactcggcct cctgatgggg cgtttggcgt cgtctgtgtg attgttggcg agtaacgaac
    16981 agccaaacca gcgctgtgtc ggatgggtta tcgtacggcc tgagtgtttg ttgtcttgga
    17041 tggcggaata agagtttcga ttcgataatc gttgagcggc aagatagttg ctttgccgtc
    17101 gaggtaaaga gcgatgcgct cgttagacga gtcaattacg aagcctttgg ctatttcgat
    17161 gtcgccatcc atcaggcgta cgcaatagtc ctgacgcttt tcgacttttt cacagcctaa
    17221 tgaaaatact gatgtgcttt tttctattgc tagttgtgct gatttgtagc cggtatatgc
    17281 cggtgtgatg aggaataagt tagctgctac aggtagcgct agtagaatta aagtaatgct
    17341 ggttgataaa gcagtgatgg tcattaatag atctaatttt ttgttcttgc taacagcgcc
    17401 gttggtggtc ttttttttgt tggggcgtga tagccaggcc agtaacataa tttcgactac
    17461 taggaaagca acagtcccta gagttgaaag gacaatccac ggatcagtca gaacgttaag
    17521 ccagttgctg catacttgga ttagggctgc gtatgcataa ataaaataat ctgtagtgga
    17581 tttctcgaag aggcccgtgc cgacactaaa gtggttaacc cagatttgat gataaaccac
    17641 accggttaag tagattgcag agtagccaag taacccgagc agtggagcga aagcaatcca
    17701 aaatatcttt acgaagattg tttcattgct ggtattttta tcaatttttt ccatgtcagc
    17761 tctcctttat attttcgcag tttgaagatt tatctacatg cactcaagcc ctgcgccatg
    17821 tcttgcagca aactaacagg caacgcggtt cccggatccc gtcaggaggc cgagtggagg
    17881 tgctgtggag aggggcgttt ggcatggatg ctaaacgagg aacgatgggc cggggatggc
    17941 ccaccgtgac gaccctcgga acagtgccag agcgagggac gttgagcgta gcgaaacccg
    18001 gatgtcgggc gcgctttctc tttggttact ttctctttgc gcgcgcatac ggagcgaagc
    18061 gcagtaacag ccgcaggctg gcccgaaggg cgagcgcagc gagtaaagag aaagtaactc
    18121 ggcgtaaagc cgaaatcggt aattgatatt actaagaagt gtggcttgtc cccgattatc
    18181 gggtactata ggcctatgaa agttttgata tgagaaagct tgttgttccg acaacaagcc
    18241 agagaagtgc caaattaacc ccggttttta gccggtataa ttttatgtct cttatgtata
    18301 ttagtttcat ttgctcaaat gcaagttctt catttatttc gtctggtgta tgtgggactt
    18361 cagctaggaa tgacttcacg tctttcgtgg cttttatttc tttccggttg aaaattgagt
    18421 caggtatgct gaattcaaac attccgccgc catagaacat gcctttgtgt ttgttggagt
    18481 taactatatg tctggctggg ttgtctattg cggagaggcc tcgcgcaaaa catagtatag
    18541 atagtgccca aagtatgaaa aaaagaaaaa ataatattaa tcctatgatt tttggctcta
    18601 tggtgcagat gtaattaaaa tgcgaccata ttcggcccag aaaagtaaat gggattagca
    18661 gtcctgttaa gatcgcgccg atttttacgt ctatcgccct tattgttgtt tgggtgtcta
    18721 gtatcgcttt ggtgatgaat tctgagttac tttgcatttt tggaaaccca ctcatccatt
    18781 tgtatgttta caacgttccc gtgatagcag tccctgttgt agttttttga gagaaggttt
    18841 ttgttagact cgttaaggtt gtcgtagaaa atatctgaaa ccataacttc tttgtctgca
    18901 tatgtcttgt ttccgtagga gcataatttt gctgcttctc caacaagttt ccctagccaa
    18961 attacctcgt tgactccact gccactatag cctgccttga tgtacaagga tgaaccgtaa
    19021 gataggccta tgcctacagt gatttccgaa taccctttct tcttgtactt tatatttaag
    19081 gtgtcaatta gtgatgatgc tttggcggct agtgagaata gattgtcaat atcttgcttg
    19141 taaggagtgt cgtacacgcc ccatacgcaa tcaccttcga tgtatatctc gctgacgttg
    19201 gagtggtctt ttagaatcgc aacaagttct gatgtatagg tcttgtatat ttttgccaaa
    19261 gttgggcgtt tgtgtttttc tgaaaggctt ttggaatctc gcatatcaac aaaaatcgca
    19321 gcgcaatcta cgtagaaccc attgctaaaa gtcaatgtgt tccttggtgg gattgatttt
    19381 ctgtcttcgt agttggcgtc ggagttgttg agtgtctggt cgattctctt aatgctttct
    19441 tcgtgattgt atgcgctgta ttttgcttcc atttttagtc ttctagtatg cctttatggg
    19501 aaacgaagag ccccgcacaa ggcggggctc ttaattttca atcgccaccc caaacatcca
    19561 tttcaggtaa agcgctctta actcttcagt gagtaagctg ggaatcaatc ccagctcaat
    19621 gccccaccag tctgatactc aataacccgc gtctcgaaga agttcttctc cttcttcagg
    19681 tccatgattt ccgacatcca tgggaacggg ttagacgtac ctgggtactc ttccttcaaa
    19741 ccaatctggg tcaggcgacg gttggcgata aatttgaggt agtcctccat catcgcggcg
    19801 ttcatgccca gtacgccgcg cggcatggtg tcgcgtgcgt attcgatttc cagctgagtg
    19861 ccttccagaa tcatctgggt tgcttcgtcc ttcatcgcgg cgtcccacag gtgtgggttt
    19921 tcgatcttga tctggttgat cacgtcgatg ccgaagttca ggtgcatgga ttcgtcgcgc
    19981 aggatgtact ggaactgctc ggcgacgccg gtcatcttgt tgcggcggcc catggagagg
    20041 atctgggtga agccgcaata gaagaagatg ccttccagta cgcagtagta ggcgatcagg
    20101 ttgcgcagca gttctttgtc ggtctcgacc gtgccggtgg tgaaggtcgg gtctgagatc
    20161 gagcgggtgt atttgaggcc ccaggctgcc tttttagcga ccgacgggat ctcgtggtac
    20221 atgttgaaga tcgcgccttc gtccatgccc agcgattcga tgcagtactg gtaggcgtgg
    20281 gtgtggatgg cttcttcgaa ggcctggcgc aggatgtact ggcggcactc ggggttggtg
    20341 atcaggcggt agacggccag caccaggttg ttggcgacca ggctgtcggc ggtggagaag
    20401 aagccgaggt tgcgcatcac gatgcggcgc tcgtcgtcgg tcaggccgtc cggagttttc
    20461 cacaacgcga tgtccgcgtt catgttcact tcctgaggca tccagtggtt ggcgcaacca
    20521 tccagatact tctgccaggc ccagtcgtac ttgaagggga cgagttggtt gaggtcggcg
    20581 cggcagttga tcatgcgctt ttcatcgacc gcgacgcggg cggaggcacc ttccagctcg
    20641 gccaggcctt cggcgatgtc gagttggtcg agggcggctt tggcgcgggc tacggcggcg
    20701 ctgtcatcgg cggcaatggc gcgggcttcg accgcggcgg caccgccgac ctggtcgagt
    20761 ttgtcgatgt tcatgtctgc catttgggca gaggctttgc ttgctacggc ggtgtcggcg
    20821 ccgtcttcct tgtcaaattc atcccagctc agcatggctt ggctcctgct tgcgggccgg
    20881 tgaaataacc ggcctgtgtg gatatacagt ctaattgtgt tccgttgcgt acagcgcgca
    20941 agggcaaaac gggtaccgct ggtcggggcg gctctagtag ctggccgttg ccccgaagcc
    21001 cacgccgggt agggcggggg ttggtaataa gtggggaggg cggtgttgcc ttttcccctc
    21061 accctaaccc tctcctagcc ggccgccccc gaaggggcga ggggactgat cgtgtgttgc
    21121 tctagctttt cgttcgacat tcaagcgtgt agctgagcga gtgagcgcta ggcgcctggt
    21181 gggttggaac ccctgagcat acgactgtat gtgatggggg ccgacctacc aggcaacaac
    21241 gcgcgcgccg ctcagatccg cgcgcttatt ggcacgcttc gcagtctggt tcgtcgatcg
    21301 cgcaagcctt tggtactgga gctgggcctg cgctcatttc cacttccggg gccttggccg
    21361 gtgctgcgct caggccatcg ttgccaccac tggacacggc gttcagcttg ccggtgttga
    21421 tggtggactt ctcggtgctg gtcgcggcca gggcacggag gtagtaggtg gttttcaggc
    21481 cacggtacca agccatgcgg taggtcacgt cgagcttctt acccgaggcg ccggcgatgt
    21541 acaggttcag cgactgcgcc tggtcgatcc acttctggcg acggctggcg gcgtcaacga
    21601 tccacttggt gtccacttcg aacgcggtgg cgtagaggtc tttgagttcc tgcgggatgc
    21661 gctcgatctg ctgcacggaa ccgtcgtagt acttgaggtc gttgatcatg accgagtccc
    21721 acaggccgcg ggctttgagg tcgcgaacca ggtacgggtt gatcacggtg aattcgcccg
    21781 agaggttcga tttcacatag aggttctggt aggtcggttc gatcgactgc gatacgccgg
    21841 tgatgttggc gatggtggcg gtcggtgcga tggccatgat gttcgagtta cggatgcctt
    21901 tctgcacacg ggcacgtacc ggggcccagt ccagggactc ggtcaggtcg acgtcgatgt
    21961 acttctggcc gcgctgctcg atgaggatct gctgcgaatc gagcggcaga atgcccttgc
    22021 tccacagcga accctggaag gtttcgtagc tgccgcgctc gtcggccagg tcacaggagg
    22081 cctggatggc gtagtagctg accgcttcca tcgacttgtc ggcaaattcc acagcggcgt
    22141 cggagccgta tgggatgtgc tgcaggtaca aagcgtcctg gaagcccatg atgccgaggc
    22201 caaccgggcg gtgacggaag ttggagttct tcgcctgggg caccgagtag tagttgatgt
    22261 cgatgacgtt atcgagcatg cgcacggcga ccttgatggt cttctgcagc ttggcggtgt
    22321 ccagcttgcc gtcgacgatg tggttcggca ggttcacgga acccaggttg cacacggcga
    22381 tttcgtcggc gttggtgttc agggtgatct cggtgcacag gttcgagctg tgcacgacgc
    22441 ctacgtgctg ctgcgggctg cgcaggttgc atgggtcttt gaaggtcagc catgggtggc
    22501 cggtttcgaa cagcatgctg agcatcttgc gccacaggtc tttggcctgg atggtcttga
    22561 acagcttgat cttgccgccg aactgggtca gggcttcgta gtattcgtag cgctcttcga
    22621 aggccttgcc ggtcaggtcg tgcaggtctg gcacttcgga cgggctgaac agggtccact
    22681 ggccgtcgtc gaacacgcgc ttcataaaca ggtctggaat ccagttcgcg gtgttcatgt
    22741 cgtgggtgcg gcgacggtcg tcaccggtgt tcttgcgcag ctcgatgaac tcttcgatgt
    22801 ccatgtgcca ggtttccagg taggcgcaga ccgcgccttt gcgcttgccg ccctggttaa
    22861 ctgcaactgc ggtgtcgttg accactttga ggaatggcac aacgccttgc gacttgccgt
    22921 tggtgccctt gatgtaggca cccagtgcgc gtaccggggt ccagtcgttg cccaggccgc
    22981 cagcgaattt gctcagcatg gcgttgtcgt ggatggcgtt gtagatgccc gacaggtcat
    23041 ccggcacggt ggtcaggtag cacgacgaca gctgcggacg cagggtgccg gcgttgaaca
    23101 gggtcggggt cgaggccatg tagtcgaagg acgacaacag gttgtagaac tcgatggcgc
    23161 ggtcttcacg ctgcttttct tcgatggcca ggcccatggc cacgcgcata aagaatactt
    23221 gcggcagttc gaagcgggtg ccgtccttgt ggatgaagta gcggtcgtag agggtctgca
    23281 ggcccaggta ggtgaactgc tgatcgcgct cgtggttgat cgctttgccg agtttttcca
    23341 gatcgaaagt ggccagcacc gggttgagca attcgtactc gatacccttg gcgatgtaag
    23401 ccggcagagc cttggcgtac agctcgctca tttcgtggtg agtggcgctg gtggcgacac
    23461 cgaggaagcc caggccttcg gcgcggatgt tgtccatcag caggcgtgcg gtgacgtagc
    23521 tgtagttcgg ctcacgctca accagggtgc gcgcggtcat caccagggcg gtgctgacgt
    23581 cgctttgcgc tacgccgtcg tagaggttct tgagggtttc tttctggatt agcgcgccgt
    23641 cgacttccgc caggccttcg caggcttcgg tgacgatggt gttgaggcgg cccatatcca
    23701 gcggcgcaac agtgccgtcg aggtgggtaa cgcgaatgct tgggtgcggc tcggcgatgt
    23761 cagtggcggc accgcttttg cgcgcctggc tgcgcgattc acggtagatc acgtagtcgc
    23821 gggcaacttt ctgctcgccg gaacgcatca gggccagttc gacctggtcc tggatttctt
    23881 cgatatggat ggtgccgccg gacggcatgc ggcgcttgaa ggtggcgctg acctgctcgg
    23941 tcaggcgctc gacggtttca tggatacgcg acgaggcggc tgcggtgcca ccttcaacag
    24001 ctagaaaggc cttggtgatg gcaacggtga tcttgtcatc ggtgtagggc accacagtac
    24061 cgttacgctt gatcacacgc agttgacccg gcgcggtggc gctcaggctc tggttgtcat
    24121 cggtggcctg cggcgcattg gcctgcgggt tctcgcggga ggtgtcggtg tgcatgaaga
    24181 tctccatgtt ctgtatttgt tagatggacg ctttaactgt cgatcactgc gaagaactgt
    24241 cgttcattcc atttcatcaa tgccatgggc acagccagag gctgggcaca ctgggacttg
    24301 caaattgggc agggaaggta cgactaaagc gccttcctgg ctcaagagtc acacgtcaag
    24361 gtgcttttgg cgcctcaact actacatcta gtggtgcaac cgcgtgtgcg gtcgctgctt
    24421 tattcatttt caggcgcgct aagcgctgca ctgaagggtg gcctgctgcg gtagagcagg
    24481 cgtactgcaa gggtctttca ggcgaccggt gacgggctgg gatcaggccg tgtgcgagtc
    24541 attcttcttg tgtccggttg ccaggctaaa cccaacatgt ggtgggtgtc gcgcgattgg
    24601 catacaagat aatgcgctat ctggggtatt gcaaggcgag agagcaagaa caacctgtgg
    24661 ataagttgtg ggtgaattgt gggttaaacc gcgcgattgg cggcaagccc gcatgcttgc
    24721 tggggtgtac agttcgtcgg tgttgatcag gcttttgctt tggtttaccc aggtgtgggc
    24781 tatctctcag ttcgatacta catctagtgt ttctacagaa atggggcatg cgctatgcag
    24841 tgcccagggc attgatacaa tgccggccgt ttccgtgttg gcaggagtcc gttgtggaac
    24901 agcagtcgtg gcacattctt attgtcgaag acgaccagcg cctggccgaa ttgacccgtg
    24961 agtacctgca gagcaatggc cttaacgtgt ccatcgagat ggacggtgcg caggccgccg
    25021 cgcggattct gcaggagcag cccgacctgg tgatcctcga tctgatgctg ccgggtgaag
    25081 acggcctatc gatctgccgc aaggtgcgtg gccaatacga ggggccgatc ctgatgctca
    25141 ctgcgcgtac cgacgatatg gaccaggtgc tgggcctgga aatgggcgcg gacgactatg
    25201 tctgcaagcc ggtgcgtccg cgcgtgctac tggcgcgtat ccgcgcactg ctgcgccgtc
    25261 atgagggcgc agaggtgcag gcgccaagcg agcgccggcg tctgcagttc ggcccgctgg
    25321 tgatcgacag cgcgatgcgt gaagcctggc tgggtgagca gagcatcgaa ttgaccagtg
    25381 ccgagttcga cctgctgtgg ctgctgactt ccaacgccgg gcggattctc tcgcgcgaag
    25441 aaatctttaa tgccctgcgc ggcatcgaat acgacggcca ggaccgttcc atcgacgtgc
    25501 gcatttcgcg catccgcccg aaaatcggcg acgacccgat gcatccacgg ctgatcaaaa
    25561 ccgtacgcag caagggctat ctgtttgttg ccgaagcggc tgaagcactg acatgaactc
    25621 attgccatga actcgatctt cctgcgcatc tatggcggca tgctcgccgt gctggtgctc
    25681 gttgcattgc tcggcgtcgg cgcgctgcaa ctgctcaacg atgtgcgagt ggatcaatac
    25741 cgtgagcgtc tggcccaggg caccttccgc ctgatggccg ataacctctc accgatggtg
    25801 gatatcgagc ggcgccgcgc ggttacggtg tgggggcgct tgctcggcat tccaatgcgg
    25861 gtcgagacgt tggaaagtgc cgggctggac agcggcgcgc gcaacagcct gcgccgcggc
    25921 caggtgctgg tgcagcagac cggcccgcac gcagcgcgca tcttcagcca gctggacgag
    25981 cagccgctgc tgctgattgc cgatgtgcag caggtcagcg aacagctggc ccgcgccacc
    26041 agctttctgc tgatcgatga gctggtgcgc tatccctggg atgaacagcc gcgccgactg
    26101 gcggcgttga aacaggacaa gcagttcggt ttcgacctgc gcctggtgcg tctcgatcag
    26161 gccgacctcg accccgatca gcgccgccgc gtggatgaag gcgacacagt gatggccctg
    26221 ggtcggggtg gcgacagtat tcatgtgttt tccggcatcg tcgaaacgcc ctgggtgctg
    26281 gaaatcggcc cgctgtacca aatgaatcct tacccggcgc actggttagt gctgatcggc
    26341 gcactgggcc tgagcctgat cgggctgatg gtctacctgc tggtgcggcc gctggagcag
    26401 cgtttgagtg gcctggaagg ggccgccacg cggatcgcca gcggccgcct ggatgctcgc
    26461 gtgccgactc gcggcagtga ttcggtgggg cgcctggcgg cggccttcaa cgccatggcc
    26521 gagcacctgc agcgctccct gagtattcag cgcgagatgg tccgcgccgt gtcccatgag
    26581 ctgcgcacgc cggtggcgcg gctgcgcttt ggcctggaga tgatcggtga tgccgagagc
    26641 gaagcggcgc ggcgcaaata tatggacggc atggacagcg atatccagga cctcgacaag
    26701 ctggtcgacg agatgctcac ctacgcacgc ctggagcagg gcgcgccggc gctgaacttc
    26761 cagtcggtcg atttgaacga a
//
LOCUS       DASRUM010000277         9559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1144778, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000277 DASRUM010000000
VERSION     DASRUM010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9559
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144778"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VFF22_18555"
     CDS             <1..472
                     /locus_tag="VFF22_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017516272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18856.1"
                     /translation="VDGNGNIIKGATTTRNDFFSINLEGFTLNGAYLNLWGDSTGLQG
                     EASLQMYTDKLVIGTCRNCTDANRLVAKNLYFDINLGQASLQPLNFSVLGDGELRLSL
                     PSVTWNNHQAFYQQVPKSNISVGNLSMGGVDLGSQVVRGMRVDYLEVRTVNLPR"
     gene            484..984
                     /locus_tag="VFF22_18560"
     CDS             484..984
                     /locus_tag="VFF22_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017940111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18857.1"
                     /translation="MRSTYLGFALLLMAGPVLAELRPLAEDELQGISGQAGLSISASL
                     NFAANPAQTRCPGGCGTRVAIQPANSSGFIVLDNIQGTFGFEGLSFDIVTIDSGYNGE
                     GAAFNESAMRLGLTDASFNNARVTLAGSNSATSGGVGFQQTDLLTYQINGDVRINGNL
                     YLFATP"
     gene            1321..2418
                     /locus_tag="VFF22_18565"
     CDS             1321..2418
                     /locus_tag="VFF22_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HZX18858.1"
                     /translation="MIKTPYYLIDKQKLLGNLEKIAYVREHSGAKALLALKCFATWSV
                     FDLMQQYMDGTTSSSLYELKLGRQKFAGEAHAYSVAWADDEIPEMLANCDKIIFNSIG
                     QLERFAEASAGKVRGLRVNPQVSSSDYLLADPARPFSRLGEWDPEKIAAVMDKVSGFM
                     FHNNCENGSFELFDQMLSTIEERFGHLLEQVEWVSLGGGIHFTGDDYPLDQFCARLKA
                     FSERFGVQVYLEPGEAAITLSSSLEVTVLDTLYNGKHLAVVDSSIEAHMLDLLIYRLN
                     AKMEPCNGEYSYMVCGKSCLAGDIFGEYQFDKPLAIGDRLSFIDAAGYTMVKKNWFNG
                     LKMPSIVVKQLDGSVEVVREFGFEDYLSSLS"
     gene            2555..3799
                     /locus_tag="VFF22_18570"
     CDS             2555..3799
                     /locus_tag="VFF22_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HZX18859.1"
                     /translation="MKKNVLIIGAGGVAKVVAHKCAQHNDELGRIAIASRNISKCQAI
                     IDSVKAKGSLKQPAEIQAYALNALDIEATKTLIRETESQIVINVGSAFLNMSVLRACI
                     DTGTAYLDTAIHEEPGKVCETPPWYGNYEWKHLAECQDKGVTAILGVGFDPGVVNAYA
                     ALAQQQYFDSIESIDILDVNAGSHGKYFATNFDPEINFREFTGQVWSWQNSQWTSNTM
                     FEVKRTDDLPVVGEQNLYLTGHDEVHSLSKHLNVPNVRFWMSFGEHYINVFTVLKNLG
                     LLSEQPVKTAEGLEVVPLKVVKAVLPDPASLAPGYSGKTCIGDLVKGIKDGKPAELFI
                     YNVADHKDAFVETDSQGISYTAGVPPVAAALLVARGDWDVAKMANVEQLPAEPFLKLL
                     DVMGLPTRIKDANGDRPWDQQV"
     gene            4070..5617
                     /locus_tag="VFF22_18575"
     CDS             4070..5617
                     /locus_tag="VFF22_18575"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HZX18860.1"
                     /translation="MSSNDRVIIFDTTLRDGEQSPGASMTGEEKLRIAKALERLRVDV
                     IEAGFAIASPGDFAAVKAIADTIKDSTVCSLSRALDADIDRAAEALKGANSGRIHTFI
                     ATSPIHMQYKLRMQPDQVVEQAVHAVKRARNLCSDVEFSCEDAGRSEIDFLCRIIEAA
                     IDAGARTINIPDTVGYAIPHQYAETIRQLLNRVPNADKAVFSVHCHNDLGLAVANSLA
                     AVVAGARQVECTINGLGERAGNAALEEIVMAIKTREDLLGVHTRIDTPHILSTSRMVS
                     GITGFPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMSAQSVGWNANKMVMGKHS
                     GRAAFRSRLDELGIALAGESELNAAFARFKALADKKHEIFDEDLQALVSDTLAEEAPE
                     HFKLVTLDVASKTGEVPQAQVVVSVDGAEHNGHAQGSGPVDATFKAIESIASSQSTLQ
                     LYSVNAITQGTDSQGEVTVRLEKGGRIVNGNGADTDILVASAKAYINALNLMQAGQKA
                     HPQAADV"
     gene            5614..6435
                     /locus_tag="VFF22_18580"
     CDS             5614..6435
                     /locus_tag="VFF22_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZX18861.1"
                     /translation="MIAELRRRAYLSAMQVARWLPRVELPFAAPSRPELLQPLIESES
                     ELEVAAAVPQAVAEAAPAHEPPLAETSVVAPVTRPKIEVPRPAPQGRMAKVEAVAADA
                     EEQVKPQPASVAPPRFALQLLRAGACAVLVELPTGEPLQGRDPAYLLLKDLLRAAGLP
                     DSPQLIGEPVRWPLLVRGQIDQGPQAALEYVQSFVGARLEEQEPCSCLWLVGLPAIRF
                     AGEATADAYNRELQVEGLGAAWALPGLELLMDEPERKRELWQAMRRVRQRWLADN"
     gene            6491..6943
                     /gene="rimI"
                     /locus_tag="VFF22_18585"
     CDS             6491..6943
                     /gene="rimI"
                     /locus_tag="VFF22_18585"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HZX18862.1"
                     /translation="MSDAISFRPMTAADIDAVLKIEYAAFSHPWTRGIFTDSLTSYNC
                     WVMFEGEQQVGHGVIQIIMDEAHLLNITVKAESQGRGLGLKLLEHLMQQAQQQGANDC
                     FLEVRASNQVAYRLYERYGFNEVGRRRDYYPAVGGREDALVMVCPLFD"
     gene            7157..9067
                     /locus_tag="VFF22_18590"
     CDS             7157..9067
                     /locus_tag="VFF22_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HZX18863.1"
                     /translation="MRLSASLLAVFCLFTAAQALSAERSFTILHTNDWQSRLLGFGPN
                     NEYSPATTGDDDTVGGVARLATLLEQRRAAAGEQPVLLLDGGDFSMGTLFHTVTRETG
                     GELRLMTELGYDAATLGNHEFDFRPAGLAAMISSAHKVKGAALMPLLSSNLGFDPTRK
                     EDDSLQAHYEAGRIAPYKLIDKGGIRFGLFGLLGNNAVAVSPMIKPAVFADPVVTAQA
                     MVAKLREEGAEVVILLSHMGVVQQEDGSWRGEEVELVEQVPGIDIVVGGHSHTALAQP
                     LLVGGRTPVMQAGSEIQHLGELRMRLSADGKPQVVDYQLHPINDQIAGSAAITAQVED
                     FKQVVSAQMLAPKGYTFDQPLAKVDKTLTRAHDDPILGNLVTDALRQATGSDVSFTGN
                     GTIRDNMIMGRHGVQAVSDLFRIAPLGVGELDDAPGYPLIKVYVSGQELKNLLEVLLL
                     AYQVRESKSYYPRVSGLRFSYNPWRVPFDRVSRIELGDPIKGYTPLDLTDQRLYSIGA
                     TSYVGSFTWLVSDLTKGLLSVQPKDAQGRPLAAIKDAIIDADSQQPGVQEYKEWQGLL
                     DHVRSLPDLDGDGLADIPTQGAAAEQRMLRATSLQPAELYRYAGPLQWGVSLVLLAGL
                     LLIIWLLRRRRA"
     gene            9155..>9559
                     /locus_tag="VFF22_18595"
     CDS             9155..>9559
                     /locus_tag="VFF22_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005758746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HZX18864.1"
                     /translation="MSDLQHLFDNNARWAEAIKEEDPEFFAKLAQQQVPEYLWIGCSD
                     ARVPANEIVGLLPGDLFVHRNVANVVLHTDLNCLSVIQYAVDVLKVKHILVTGHYGCG
                     GVRASMQDTQFGLIDGWLRSIRDLYYEHREHLA"
BASE COUNT         1864 a         2928 c         2912 g         1855 t
ORIGIN      
        1 cgtggacggc aacggcaata tcatcaaagg tgccaccacc acccgcaacg attttttttc
       61 catcaacctg gagggtttca ccctcaatgg ggcctacctc aacctgtggg gtgacagcac
      121 cggactgcag ggcgaggcca gtttgcagat gtacaccgat aaactagtta ttggtacttg
      181 tcgcaactgt accgatgcca atcgtcttgt ggccaagaac ctgtatttcg atatcaacct
      241 cggtcaggcc agcctgcaac cgctgaactt ctcggttctg ggcgatggtg agctgcgtct
      301 gtccctgccc agcgtcacct ggaacaacca tcaggccttc taccagcagg tgccgaagag
      361 caatatcagt gtcggcaacc taagcatggg tggggtcgac ctgggttcgc aagtcgtgcg
      421 tggtatgcgt gtggattatc tggaagtgcg taccgtgaac ctgccgcgat gaggagagtc
      481 agtatgcgca gtacatacct aggctttgcc ctgctgctta tggcgggacc cgtgctggcc
      541 gagttgcggc cgctggctga ggatgagttg caggggataa gcgggcaggc ggggctgagt
      601 atcagtgcca gcctgaattt tgccgccaac cccgcgcaga cccgttgccc gggtggttgt
      661 ggtacccggg tggcgataca accggctaac agcagcggtt ttatcgtgct ggacaatatt
      721 caaggtacct tcggctttga ggggctgagc ttcgatatcg tcaccatcga cagcggatac
      781 aacggtgaag gggccgcctt caatgaatcg gccatgcgcc tggggttgac cgatgcgagc
      841 tttaacaatg cccgtgtcac cttggccggc tccaatagcg ccacgtccgg cggggtgggc
      901 ttccagcaga ccgatctgct gacctatcag atcaatggcg atgtgcgcat aaacggtaac
      961 ctctacctgt tcgccacgcc ctgagtcgct ctgcgccgag gccggcggcg ccgacttgag
     1021 cccctgccaa ctggcgggtt gggccaattt ccggtctatc tgactgttac ctttctgtca
     1081 tcttgctgcc atggttgccc ttcattctgg cggccatact gtcgccgaat ctgtgctgcg
     1141 atgttcgcgc gactggtcga taaagcatcg ccaatggtcg ctgaaccgag gaaatatccc
     1201 tgttgctctt tcgatatgtg ccggttgtcg gcacaattcg cctccacttt tcggggaggt
     1261 gtcagctttg ctgattgcct gccggccgac tttgctagca ctcagggcat agacacgcag
     1321 atgatcaaga cgccgtacta cctcatcgac aagcaaaagc tcttgggcaa tctggagaag
     1381 atcgcctatg tacgcgagca ctccggcgcc aaggcgctgc tggcgctcaa gtgctttgcc
     1441 acctggtcgg tatttgacct gatgcagcaa tacatggacg gcaccacctc cagctcactg
     1501 tatgagctca agctcggtcg gcagaagttc gccggtgaag cccacgccta cagcgtggcc
     1561 tgggcggatg acgaaatccc cgagatgctc gccaactgcg acaagatcat cttcaactcc
     1621 atcggccagc tcgagcgctt cgccgaagcc tccgccggca aggtgcgcgg cctgcgcgtt
     1681 aacccgcaag tgagcagctc ggattacctg ctggccgacc cggcgcgccc attcagccgc
     1741 ctcggcgaat gggatccgga aaagatcgct gcggtgatgg ataaggtcag cggctttatg
     1801 ttccacaaca actgtgagaa cggcagcttt gagctgtttg accagatgct gagcactatt
     1861 gaagagcgct tcggccacct gcttgagcag gtcgagtggg tcagcctcgg tggaggcatt
     1921 cacttcaccg gcgacgacta tccgctggat cagttctgcg cacggctcaa ggccttctcc
     1981 gagcgcttcg gcgtgcaggt gtatctcgag cccggcgaag cggctatcac cctgagctcc
     2041 tcgctggaag tcacggtgct cgacaccctg tacaacggca agcatctggc cgtggtcgac
     2101 agctcgatcg aagcgcacat gctcgatctg ctgatctacc gccttaacgc caagatggag
     2161 ccctgcaacg gtgaatacag ctacatggtg tgcggcaagt cgtgcttggc gggtgacatc
     2221 ttcggtgagt accaattcga caagccactg gccattggtg atcgcctgtc gtttatcgat
     2281 gccgccggtt acaccatggt caagaagaac tggttcaacg gcctgaaaat gccgtccatc
     2341 gtggtcaagc aactcgacgg cagtgtcgag gtggttcgcg aatttggctt tgaggactac
     2401 ctctccagcc tgtcgtaaaa cttgtagggt ggacgacgcc tttttcgtcc accgctcggc
     2461 aatcgcgcag gtggatgaaa acagcgtcat ccaccctagg tttgtaaatc cgcaaaaact
     2521 gtctcaataa cgcagtaccc aggaggtgaa acaattgaag aagaatgttc tgatcattgg
     2581 tgcaggaggt gtcgccaagg tggtggccca caagtgcgcg cagcacaacg acgaactcgg
     2641 tcgtattgct atcgcgtcgc gcaacatctc caaatgccag gccatcatcg acagcgtaaa
     2701 ggccaaaggc agcttgaagc agccggccga aattcaggcc tatgccctga atgcgctgga
     2761 tattgaagca accaaaacgc tgattcgcga aaccgaatcg cagatcgtta tcaacgtggg
     2821 ctcggccttc ctcaatatgt cggtgctgcg cgcctgcatc gataccggca ccgcctacct
     2881 cgacaccgcc atccacgaag agccgggcaa ggtctgcgaa acaccgcctt ggtacggtaa
     2941 ctacgagtgg aagcacctgg ccgaatgcca ggataagggc gtgaccgcca tcctcggcgt
     3001 cggtttcgac ccgggcgtag tcaatgccta tgccgcattg gcgcaacagc agtacttcga
     3061 cagcatcgag tcgatcgaca tcctcgacgt caacgccggt tcccacggca agtacttcgc
     3121 caccaatttc gacccggaaa tcaatttccg tgaattcacc gggcaagtct ggagctggca
     3181 gaacagccag tggaccagca acacaatgtt tgaagtcaaa cgcaccgacg acctgccagt
     3241 ggtcggcgag cagaatctgt acctgaccgg gcatgacgaa gtgcattccc tgtccaagca
     3301 cctgaatgta cccaatgtgc gcttctggat gagcttcggt gagcactaca tcaatgtgtt
     3361 taccgtgctg aaaaacctcg gcctgctctc cgagcaaccg gtaaaaaccg ccgaaggcct
     3421 cgaagttgtg ccactgaaag tggtcaaggc cgtactgccg gacccagcct cgctggcgcc
     3481 gggctacagc ggcaagacct gcatcggcga tctggtcaaa ggcatcaagg acggcaagcc
     3541 ggccgagctg tttatctaca acgtcgccga ccacaaggac gcgtttgtgg aaaccgacag
     3601 ccagggtatt tcctataccg caggcgtacc gccggtcgca gctgcactgc tggtcgctcg
     3661 tggtgattgg gacgtggcga agatggccaa cgttgaacag ctgccggctg agccgttcct
     3721 caagctgctc gatgtaatgg gcttgccgac acgcatcaag gacgccaatg gcgaccgtcc
     3781 gtgggatcaa caggtctgat tggctgcgtc tagctcgttg gattagccta acgcccgcca
     3841 tttggcgggc gttttgcttt ttggtgaaaa tccaccgtcg cctgttactg gtggatgtaa
     3901 agagcgacat ccaccctacg tgcaggatgc gcgatcaaac cgtgggaggg gctttagccg
     3961 cgatgcgcgc cgcgcttccc gcccgtggca tttcacgcga caatcaaccc ttatcgacac
     4021 gcgccccaac gtcgggcgcg tgctacggcg actccgtgag gaaaccagca tgagcagcaa
     4081 cgaccgcgtc attattttcg ataccaccct gcgtgacggc gagcagagcc ccggcgcgtc
     4141 gatgaccggt gaagagaagc tgcgcatcgc caaggctctg gagcgtttgc gcgtcgacgt
     4201 gatcgaggcc ggcttcgcca tcgccagccc aggcgacttt gctgcggtca aggcgattgc
     4261 cgataccatc aaggacagca ctgtgtgcag cctatcgcgg gctctggacg ccgatatcga
     4321 ccgcgccgcc gaagcgctca agggcgccaa ctccgggcgc atccatacct ttatcgccac
     4381 cagcccgatt catatgcaat ataagctgcg tatgcagccg gatcaggtgg tcgagcaggc
     4441 tgtgcacgcg gttaaacgcg cgcggaatct gtgcagcgat gtcgagttct cctgcgagga
     4501 tgccggccgt tcggagatcg atttcctctg ccgcattatc gaggcggcga tcgacgccgg
     4561 cgcgcgcacc atcaatatcc ccgataccgt cggttacgcg attccgcacc agtacgccga
     4621 gaccattcgc cagttgctca accgcgtgcc caacgccgac aaggcagtgt tctccgtgca
     4681 ttgccacaac gacctgggcc tggccgtggc caactccctg gctgccgtgg tcgctggtgc
     4741 ccgtcaggtc gagtgcacca taaacggtct cggtgagcgc gccggtaacg ccgcgctgga
     4801 agagatcgtt atggcgatca agacccgtga agacctgctc ggcgtgcaca cgcgcatcga
     4861 caccccgcat atcctcagca cctcgcgcat ggtttccggc atcactggtt tcccggtgca
     4921 gccgaacaag gccatcgtcg gcgccaacgc cttcgctcac gagtcgggca ttcaccagga
     4981 cggcgtgctc aagcaccgcg aaacctacga gatcatgtcc gcgcagtcgg tcggctggaa
     5041 cgccaacaag atggtcatgg gtaagcactc tgggcgtgcg gccttccgct cgcgtctgga
     5101 tgagctgggt atcgctctgg ccggcgaatc cgaactgaac gcggccttcg cccgcttcaa
     5161 ggcgctggcg gacaagaaac acgagatatt cgacgaagac ctgcaggctc tagtctccga
     5221 caccctggcc gaggaagcgc ccgagcactt caaactggtg accctggatg tcgccagcaa
     5281 gaccggcgaa gtgccacagg cgcaggtggt ggtcagcgtc gacggcgccg agcacaacgg
     5341 ccacgcgcaa ggttcggggc cggtggatgc gaccttcaaa gccattgagt cgatcgccag
     5401 ctctcagtcc accctgcagc tgtattcggt caacgccatc acccagggca ccgattcgca
     5461 aggcgaggtg actgtgcgcc tggagaaggg cgggcgcatc gtcaacggta atggcgccga
     5521 taccgacatt ctggtggctt cggccaaggc ctatatcaat gcgctgaacc tgatgcaggc
     5581 cgggcaaaaa gcccatccac aggcggctga cgtttgattg cagaactgcg ccgtcgtgcc
     5641 tatctgagcg ccatgcaggt ggcccgctgg ctgccgcgtg tggagctgcc gtttgccgcg
     5701 ccttcgcgcc cggagctgct gcaaccgctc attgagtccg agtccgagct ggaggtagcc
     5761 gctgccgtgc cacaggcggt ggctgaagcg gcgccggccc atgagccgcc gcttgccgaa
     5821 acctccgttg tcgcgcctgt tacccggccc aagatcgaag tgccacggcc ggcgcctcag
     5881 gggcgcatgg ccaaggttga agccgttgca gctgacgccg aagagcaggt gaaaccccag
     5941 ccagcgtccg tcgcaccacc gcgctttgcc ttgcaattgc tgcgcgccgg cgcttgtgcg
     6001 gtgctggtcg aactgccgac cggcgagccg ttgcaaggcc gcgacccggc ttatctgctg
     6061 ctcaaggatt tgctgcgcgc cgccggcctg ccggacagcc cacaactgat tggcgagccg
     6121 gtgcgctggc cgctgctggt gcgcgggcag atcgaccagg gcccgcaagc agcgctggaa
     6181 tatgtgcaga gctttgttgg cgcccggctg gaagagcagg agccctgcag ctgtctgtgg
     6241 ctggtgggct tgcccgccat tcgctttgcc ggtgaggcca ctgccgacgc ctataaccgt
     6301 gagctgcagg tcgaggggct gggagctgcc tgggcgctgc ctggcctgga gttgctgatg
     6361 gatgagcccg agcgcaagcg cgagctttgg caggccatgc gccgcgtccg ccagcgctgg
     6421 ttggccgaca attgaataca tcttcccggt ctgcggaccg aggtttctac tgcttgccga
     6481 gtcaacctga atgagtgatg caatcagctt ccgcccgatg actgcggcgg atatcgatgc
     6541 ggtgctgaaa atcgaatacg ccgccttcag ccatccctgg acgcgcggca tctttaccga
     6601 cagcctgacc tcctacaact gctgggtgat gttcgagggc gagcagcagg tgggccacgg
     6661 cgtgatccag atcattatgg acgaggcgca cctgctcaat atcaccgtca aggccgaaag
     6721 ccagggccgc ggtctaggcc tcaagctgct cgagcacctg atgcagcaag cccagcagca
     6781 aggcgccaat gactgctttc tggaagtgcg agcgagcaac caggtggcct accgcctgta
     6841 cgagcgttac ggctttaatg aagtcggcag gcgccgcgac tactacccgg ccgttggcgg
     6901 ccgcgaagat gccctggtga tggtctgtcc gctgttcgac tagcgtcatc ctatcgcgtg
     6961 cctattttgt gggaggggct ttagccgcga atggctttga acggcttagc gtatcgcgac
     7021 cctcgcgcca ggcgctgacc cgattggcta ccgaccgcgg cgctatccgc cgctgacctg
     7081 acgcagggct tgtcgcatac tggcgcgcgc tgccttagcc gcagcggtat tcggcccatt
     7141 gccctaagga tttgtcatgc gcctgtctgc ttcgttgctc gcggtgttct gcctattcac
     7201 cgccgctcag gccctcagtg ccgaacgcag ctttaccatc ctgcacacca acgactggca
     7261 atcacgcctg ctcggctttg gcccgaacaa cgaatattcc cccgccacca ccggcgatga
     7321 cgacaccgtc ggcggagtcg cacgtctggc taccttgctt gaacagcgcc gtgccgcagc
     7381 cggcgaacag ccggtgctgc tgctcgatgg tggcgacttc agcatgggca cgctatttca
     7441 taccgtgacc cgcgaaaccg gcggcgagct gcgcctgatg accgagctgg gctacgacgc
     7501 tgcgaccctg ggcaaccacg aattcgattt tcgtccggcc ggtttggccg cgatgatcag
     7561 ctcggcgcat aaggtcaaag gcgctgcgct gatgccgctg ctgtcgagca acctcggctt
     7621 tgaccctacg cgtaaggaag acgacagcct gcaggcgcac tacgaggccg ggcgcattgc
     7681 accgtacaag ctgatcgaca agggcggcat tcgttttggc ctgttcggcc tgctcggcaa
     7741 caacgccgtg gcggtcagcc cgatgatcaa gccggcagtg tttgccgatc cggtagtgac
     7801 tgcccaggcc atggtcgcca agttgcgcga ggagggcgca gaggtggtga ttctgctttc
     7861 gcacatgggt gtggtgcagc aggaagacgg cagctggcgc ggtgaggagg tcgagctggt
     7921 tgagcaggtg ccgggcatcg atatcgtcgt cggtggccac tcgcacaccg cgctggcgca
     7981 accgctgctg gtgggtggcc gcacgccggt gatgcaggcc ggttcggaaa tccagcacct
     8041 cggcgagctg cgcatgcgct tatcagccga tggcaagccg caggttgtcg attaccagtt
     8101 gcacccgatc aatgaccaga ttgccggcag cgccgcgatc accgcccagg tcgaggactt
     8161 caaacaggtg gtcagcgcgc agatgctcgc gcccaagggg tacaccttcg atcagccgct
     8221 ggccaaggtc gacaaaaccc tgacccgcgc ccatgacgat ccgattctcg gcaatctggt
     8281 caccgacgcc ttgcgccagg ccacgggcag cgatgtgtca ttcaccggta acggcaccat
     8341 tcgcgacaac atgatcatgg gccggcatgg cgtgcaggca gtgtctgatc tgtttcgcat
     8401 cgctccgcta ggtgttggcg aactggatga tgcgccgggt tatccgctga tcaaggtgta
     8461 cgtcagcggg caggaactga agaacctgct cgaagtgctg ctgctggcct atcaggtgcg
     8521 cgaaagcaaa agctattacc cgcgcgtgtc gggcctgcgt tttagctaca acccctggcg
     8581 cgtgccgttt gaccgggtca gccggattga actgggcgat ccgatcaagg gctacacgcc
     8641 gctcgatctg accgatcagc gcctctacag catcggcgcc accagctatg ttggcagctt
     8701 tacctggctg gtcagcgacc tgaccaaggg cctgctcagc gtacagccaa aggatgccca
     8761 gggccggcca ctggcggcga tcaaggatgc aatcattgac gccgactcgc agcagcccgg
     8821 cgtgcaggaa tataaagagt ggcagggcct gctcgaccat gtccgcagcc tgcccgatct
     8881 ggacggcgac ggcctggccg atatcccgac ccagggcgcg gccgccgagc agcgcatgct
     8941 gcgtgcgacc agcctgcagc cggccgagct gtaccgctat gccgggccgc tgcagtgggg
     9001 cgtgagcctg gtgctgttgg cgggcttgct gctgattatc tggttgctac gtcgccgccg
     9061 cgcctgagca ggctgctgtg attgagagtc gcgcgtgctt gtcaaaagcc cgtggcgctc
     9121 ctatggtgcg catcgacgaa cctgggagtg aagcatgagc gatctacaac atctcttcga
     9181 taacaacgca cgttgggccg aagcgatcaa ggaagaagat cctgagttct tcgccaagct
     9241 ggcccagcag caggtgcccg agtacctgtg gatcggctgc tccgatgcgc gggtgccggc
     9301 caacgagatt gtcggcttgc tgccgggcga tctgttcgtg caccgcaacg tcgccaacgt
     9361 ggtgctgcac accgacctca actgcctgtc ggtgatccag tacgcggtcg atgtgcttaa
     9421 ggtcaaacat atcctggtta ccgggcacta cggttgtggc ggcgtacgcg catcgatgca
     9481 ggacacccag ttcggcctga tcgacggttg gctgcgcagc attcgcgacc tctactacga
     9541 gcaccgcgaa cacctggcg
//
LOCUS       DASRUM010000278        11502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_480681, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000278 DASRUM010000000
VERSION     DASRUM010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 11502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11502
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480681"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(370..1131)
                     /locus_tag="VFF22_18600"
                     /pseudo
     CDS             complement(370..1131)
                     /locus_tag="VFF22_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619197.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(1132..2061)
                     /locus_tag="VFF22_18605"
     CDS             complement(1132..2061)
                     /locus_tag="VFF22_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017679240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX18865.1"
                     /translation="MNAPVEFTAPVADQPLLRSVLRTSLRLLFRGLVRPPMPIAGQRA
                     VLRLLTAASPAPRGITRSTGTLGGRPCEWHRPQSPRGTVLLYLHGGAYLIGGSNTHRA
                     ICSNLAKRGQMEVCALDYRLAPEHQYPAAREDALAAFQELIKLGYKPEQIVIGGDSAG
                     GNLSLITSLRLRDLGLPQPAALVCFSPVTDFTGTQLHEPPAGDPLIHRKWVEQALALY
                     CPAGLAHDDPGLSPQFADLRGLAPLLIQVGEDEILLNDSLRFADKAKAAGVDVRLQRY
                     PDLWHVFQAHAGMLKAADFALAEVVSFLRERGC"
     gene            complement(2045..3520)
                     /locus_tag="VFF22_18610"
     CDS             complement(2045..3520)
                     /locus_tag="VFF22_18610"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZX18866.1"
                     /translation="MHNTSQNQASLRVLIIGSGFGGLGMAIQLQRAGIEDFLILEKAA
                     EIGGTWRDNSYPGAACDVPSHLYSFSFEPKTDWSRKFAPQAEIHGYMLRCVAKYQLRS
                     RIRCNAEVASAAFDAAAALWRVTLSNGEELCAEVLISACGQLNKPAYPALPGIEQFAG
                     EAFHSARWRHDLDLNGKRVAVIGTGASAIQFVPQIQPHVAELKLFQRSPAYVLAKPDR
                     AYRPWELAVMRRLPWLQRIDRGLKYLQHEVRGLAFISMPWLMTLFRFSFQRHLTQHIS
                     DPQLRTQLQPNYPMGCKRILISNDYFPALAQPNVAVVSEAISRVGERAIITADGREHP
                     CDVLIYGTGFTATDFLAPMQIRGLGGQELNQAWQGGAEAYKGISVSGFPNLFILYGPN
                     TNLGHNSIIYMLESQFRYVFGCIDLLRQQGVRYLDVKAAVQQRYNQQVQASSHRTIWE
                     QGCSSWYKTASGKSTNNWPGYTFTYRLQTRAPELDDYECTR"
     gene            3640..4662
                     /locus_tag="VFF22_18615"
     CDS             3640..4662
                     /locus_tag="VFF22_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZX18867.1"
                     /translation="MSTAPYTLPRRSITSVQLLTQLGLDHGLSVERCLLNTGLSPLQL
                     AELHTEIDGDQELRLIRNLIAALPEQTDLGLQAGLRYQLTTYGIWGYAMLSSQTFRQA
                     ADLGLRYLDLTFAFNRITLREDASHAHLQLDGSHLPDQVRDFLLLRDTVAVMVIQREL
                     TGAKIPLSRVQLSMPAPADPRRFIEQFGLLPEFACAQNRISFPRHLLDLPLPRANPHN
                     AQLCELQCQALLAKRQVRSGLAGQIRDRLLSTPGRLPDMELIANELNISSRTLRRRLE
                     DQGSSFRQLQEEVRQALAEELLAIASLSLEEIAERLGYSEVSNFLHAFKRWKGLTPGQ
                     YRQELA"
     gene            4792..6234
                     /locus_tag="VFF22_18620"
     CDS             4792..6234
                     /locus_tag="VFF22_18620"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX18868.1"
                     /translation="MRQGFLGQFLPHDLTDQTSILLARTLAFSGLCSVVIGLYSCIKW
                     GRMGNDALMYGSLLLVLGMPLMLLVLRSAVLPLAVAANMALACMASYSMLLIYQLGGL
                     HSAHIYWPAVIIVFAYLLAGARSATVWSLVQVLFVFWLIRLERSGASLPVFELSPRDA
                     AVNTYSGYILPVLTVWLAQWYSAQLRNQALADAELALSESREFGQRAARNQEQLGGIV
                     DDVRSTASDLLQMAGQLQQTLGGIRQRCQSIDTDVQQQADAMHQLDLALHEVLDSLAS
                     STDHMQQLSQDTQHSSSQVNACAQRMEDAQASMQAIQQSNQRIAESMQMISAIAQQTN
                     LLALNAAIEAARAGEHGRGFAVVADEVRNLSRRSNETADTVQNVLGQSQQIIDTGATQ
                     VSEVGTALSSNAGLTDNLSSSILLHNQALTRAHQQLLHVRDNSAAQRDASQRQRAASA
                     ELLDAQESLVQLGEQLEQLSHQLHQRVASA"
     gene            6334..7218
                     /locus_tag="VFF22_18625"
     CDS             6334..7218
                     /locus_tag="VFF22_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZX18869.1"
                     /translation="MSQSTNSLLLSYQPPWQWQQFHAHFALRALAGVESLQPHSYARS
                     VHIDGQRGWFRVQALADQHALQLQYSQSLQPCLATLTARVRKMFDLDCQPQQISTHFA
                     NDAQLGPLVARNPGLRLPTAFEPFEQAVRAIVGQQVTVKAAVTITGRLVTRLGEALQF
                     ADAPDIQRLFPSPTAIAEANLDGIGMPGKRVQTLQHFAAQLASGDLRLDLQAGYTAFV
                     EQLCTLPGIGPWTAEYIALRAFGEVDAFPASDLGLLKAPVWGASGIDAKQLKARAEAW
                     RPWRAYAAAHLWHNYSEG"
     gene            7223..7720
                     /locus_tag="VFF22_18630"
     CDS             7223..7720
                     /locus_tag="VFF22_18630"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZX18870.1"
                     /translation="MFYRYHDSPIGRLLLAADETGLQQLLMDIDDKPWQIGEHWRSAA
                     DELDEVCRQLDEYFARRRQRFEVRLAPQGTAFQQAVWQALQTIPFGQTSCYSALAAEI
                     ARPKAVRAVGAANGANPIAIIIPCHRVIGRDGSLTGYAGGLPRKALLLKLEGAQLPEQ
                     RLLSI"
     gene            complement(7734..8288)
                     /locus_tag="VFF22_18635"
     CDS             complement(7734..8288)
                     /locus_tag="VFF22_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZX18871.1"
                     /translation="MRRAGAWLLALCLLSGAVQASDKLAWTALREGRAVLLLRHAMAP
                     GTGDPANFQLSDCRTQRNLNQLGREQAQRWGQLLGSQWIEPRLLSSRWCRAQDTAREM
                     GLGAVEALPALDSFFQTPHTAREQTAALIEQINAQPRGASLVLVSHQVNITALTGIYP
                     ASGEGLILALPLEAPARLLARVQP"
     gene            complement(8291..8482)
                     /locus_tag="VFF22_18640"
     CDS             complement(8291..8482)
                     /locus_tag="VFF22_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009050190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HZX18872.1"
                     /translation="MGSTFNGLLGLVILALDIWAILSVIKSNAETGIKVLWILLIVLL
                     PVIGLIIWALMGPRGTVKF"
     gene            complement(8558..9106)
                     /locus_tag="VFF22_18645"
     CDS             complement(8558..9106)
                     /locus_tag="VFF22_18645"
                     /inference="COORDINATES: protein motif:HMM:NF015065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone dioxygenase"
                     /protein_id="HZX18873.1"
                     /translation="MSSLTVYHQSSPEYPNKLLTHVEDIASTLAAVGVQFSQVPVEQP
                     VTAGTANYELMDACRVQIAQLMVTHGYVSAEVLSLCDERGEGSELARALRQEQTCKPA
                     HLHYYLAGRGLLALHIGEYVYSLLCEKNDLLLLPAGTAHWFDGGEHPRFAVLRLFDSP
                     QVPGFVPVEGDFAAAFAGLDDC"
     gene            complement(9159..10310)
                     /locus_tag="VFF22_18650"
     CDS             complement(9159..10310)
                     /locus_tag="VFF22_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX18874.1"
                     /translation="MSPVLPYWRLSGFYFFYFSLLGATAPFLALYFDHLGFSAARIGE
                     LIAIPMLMRCIAPNIWGWLGDHTGQRLAIVRFGALCTLLCFAGIFISQSYAWLALIMA
                     LHAFFWHAVLPQFEVITFAHLKEQAARYSQIRLWGSIGFIVAVVGLGKLFEVLSLDAY
                     PWALILIMSGIVLSSWWVPNAQPQMRPNTLEQGGFLQQLRRPGILAFYLSAGLMQLSN
                     GPYYTFLSLHLEALGYSRGVIGQLWALGVVAEIVLFMVMARLLARYSLRTVLLASFLI
                     TAVRWLLLGNLAGHLPVLLFAQLMHAATFGSFHAAAMHFVQRSFADRQQGQGQALYAA
                     LAGVGGALGALYSGYSWHSLGAAWTFAIASLIALAAAVITARYMRDERP"
     gene            complement(10369..11460)
                     /gene="aroC"
                     /locus_tag="VFF22_18655"
     CDS             complement(10369..11460)
                     /gene="aroC"
                     /locus_tag="VFF22_18655"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HZX18875.1"
                     /translation="MSGNTYGKLFTVTTAGESHGPALVAIVDGCPPGLEINLEDLQRD
                     LDRRKPGTSRHTTQRQEADEVEILAGVFEGKTTGCSIGLLIRNTDQKSKDYSAIKDSF
                     RPAHADYTYHHKYGVRDYRGGGRSSARETAMRVAAGAIAKKYLATQGIVIRGYMSQLG
                     PIEIPFKTWDSVEQNAFFSPDPDKVPELEAYMDQLRRDQDSVGAKITVVAEGVMPGLG
                     EPIFDRLDAELAHALMSINAVKGVEIGAGFASVAQRGTEHRDEMTPEGFLSNNAGGIL
                     GGLSSGQPIVAHLALKPTSSITTPGRSIDVHGNPVDIITKGRHDPCVGIRATPIAEAM
                     MAIVLMDHLLRHRAQNADVRVSTPVLPQL"
BASE COUNT         2350 a         3688 c         3448 g         2016 t
ORIGIN      
        1 tcgaagccac cctccttcat ccgccgtaaa accccgttac tgacatcatc gtgcaaggct
       61 gtgctcatga agtgacctcc tcaggcgatg gacaaattta caggacgcta aagaaccaac
      121 taggtgtcga tactgcagtg caacgacctg gtaaacgctt tcccattgct accgccagag
      181 cctcagcggt gttgcggcac cgattgcagt gccgcccgag aatcaagcga tgtactgctt
      241 gatatgtcat aagccagacc tgaaaccagg gctggtcaac gcaggcttgt tcagagcgaa
      301 caagtcagaa gcgttaggtg cagactagtc ccatcaacac gcatttgccc agcgaaactt
      361 gtttcaggcc tattcgcgcc ccagccaagc catgatgcgc cggctcaacc aaacgggcgt
      421 cagttggccg gaccaataca gcagcttgaa ttgcaggctg atcggccggt gtaccgcact
      481 gctccgggtt tgcgcccagg cagcctgggc cacggcgtcg gcctcaaggt taacccccaa
      541 acgacgcaaa accggcgcct ggaagcgttg gttattgagc atcggcgtgc tgacaaacgg
      601 cggcatcaga tcgccgacgc ggatgccgtg cttggcccat tctagatcca gcgcctcggt
      661 cagcccgcgc acggcgaact tggaggccga gtaactggcc agatgcggta cgccatacag
      721 cccagaggcc gagcccatat tgatcaccta ggcgtcgggc gtggctttca ggtaggggaa
      781 ggctgcgtgg gtcatattca gcaggccctg cacgttgatc tgcaggatgc gcgcatgctc
      841 gctcagctcg atctcttcga agtgcccgca ctgcagaatc ccggcgctgt tgatcagcag
      901 gcgcaactgg cctccatgca gggcgcagaa gtctgccagg gcgaccttga ccgctgccgg
      961 gtcagccaca tccagttcgg catgccagac gttgtccagc tcaccggcca gcttggccag
     1021 cgcctggtgg ttgatatcca gcaagccgac ctgccagcca tgttgatgga acagcctggc
     1081 ggtggctgcg ccgatccccg aggcggcgcc ggtaatcaga atgttgttca ctcagcagcc
     1141 ccgctcacgc agaaagctca ctacttcggc cagggcaaaa tcggcggcct tgagcatgcc
     1201 ggcgtgagcc tggaacacat gccacaggtc cggataacgc tgcaggcgca catccacacc
     1261 ggcggccttg gccttgtcgg caaagcgcag gctgtcatta agcagaattt cgtcttcacc
     1321 cacctgaatc agcagcgggg ccaggccgcg tagatcggca aactgcggcg acaaaccagg
     1381 gtcgtcatgc gccaatcccg ccgggcaata cagcgccagg gcctgctcca cccacttgcg
     1441 gtggatcagc ggatcacccg ctggcggctc gtgcagctgc gtgccggtaa agtcggtgac
     1501 cggtgaaaaa cacaccagcg ccgccggctg cggcaagccc aggtcgcgca ggcgcaggct
     1561 ggtgatcagg ctcaggttgc cgccggccga atcgccgccg atcacaatct gctcaggctt
     1621 gtaacccagc ttgatcaact cctgaaacgc tgccaacgca tcttcgcgag ccgcagggta
     1681 ttgatgctcc ggggccaggc ggtaatccag ggcgcagacc tccatctgcc cgcgcttggc
     1741 gaggttggag cagatcgccc gatgggtatt cgacccgccg atcaggtaag cgccgccatg
     1801 caggtaaagc agcaccgtac cgcgtgggct ttgtggacga tgccactcgc agggccgccc
     1861 gcccaaggtg ccggtgctac gggttatgcc acgcggcgcc gggctggctg ctgtcagcag
     1921 acgcagcacc gcgcgctgcc cggcaatcgg catgggtggg cgcaccaggc cacggaatag
     1981 caggcgcagg ctggtacgca agacgctgcg cagcagcggc tgatcggcca ctggagcggt
     2041 gaattcaacg ggtgcattca tagtcatcaa gctccggggc gcgggtctgc aggcgatagg
     2101 taaaggtata gcccggccag ttattggtgc ttttgccgga ggcggttttg taccaacttg
     2161 agcagccctg ctcccatatc gttcgatgtg agctggcttg cacctgctgg ttgtagcgct
     2221 gttgcaccgc agcctttacg tcgaggtagc gtacgccctg ctggcgcagc aaatcgatgc
     2281 agccgaacac ataacggaac tggctttcga gcatatagat gatcgagtta tgccccaggt
     2341 tggtattagg cccgtagagg ataaacaggt tgggaaaacc gctgacgctg atgcccttgt
     2401 aggcttcggc gccgccctgc caggcctggt tcagctcctg cccaccgagc ccgcgaatct
     2461 gcatcggcgc aagaaaatcc gtggcggtaa atccggtgcc gtagatcagt acatcgcaag
     2521 ggtgctcgcg gccatcggcg gtgatgatgg cgcgctcgcc gacgcggctg atcgcctcgc
     2581 tgacaaccgc cacattgggc tgcgccaggg ccgggaagta gtcattgctg atcaggatgc
     2641 gcttgcagcc catggggtaa tttggctgca gctgggtgcg cagctgcgga tcactgatgt
     2701 gctgcgtgag gtgacgctgg aagctgaagc gaaacagcgt catcaaccac ggcatgctga
     2761 taaacgccaa accgcgcacc tcatgctgca ggtacttaag gccacggtcg atgcgctgca
     2821 gccatggcag ccgacgcatc accgccagtt cccacggccg ataggcgcgg tccggcttgg
     2881 ccagcacata ggccggcgag cgttgaaaca acttgagttc ggccacatgc ggctgaatct
     2941 gcgggacaaa ctgaatcgcc gaggcgccgg tgccgatcac cgccacgcgc ttgccgttca
     3001 ggtccaggtc atgccgccag cgcgccgagt gaaaggcctc cccggcgaac tgctcgatcc
     3061 ccggcaacgc cggataggcc ggcttattga gctgaccgca ggcgctgatc agcacctcgg
     3121 cgcagagttc ttcgccattg ctcagggtca cccgccacag cgcagcggct gcatcgaatg
     3181 cggcagacgc cacctcggca ttgcagcgaa tacgcgagcg cagctggtac ttggccacgc
     3241 agcgcagcat gtagccatgg atttccgcct ggggcgcaaa cttgcggctc cagtcggttt
     3301 tcggctcgaa ggaaaacgaa tacaggtgcg acggcacatc gcaggccgcc cctgggtagc
     3361 tgttgtcacg ccaggtgccg ccgatttcgg ctgccttctc gaggatcaaa aagtcctcga
     3421 ttccagccct ctgtaactga atggccatgc ccaggccgcc aaagccgctg ccgataatca
     3481 gcacgcgcaa ggaagcctgg ttctgggaag tgttgtgcat ttttatatcc ccaaccgcaa
     3541 acagtgcgga tgccaggatg ctacttgcct ggcctctggc cggttatggc atagtcggcc
     3601 aacggattgt gactttcgga caaactaccg gataactgca tgtcgaccgc cccctacacc
     3661 ctgccacggc gcagcatcac cagcgtgcaa ctgctcaccc aactgggcct cgaccacggc
     3721 ctgagtgttg aacgctgcct gctcaatacc ggcctcagcc ccctgcaatt ggccgagctg
     3781 cacactgaaa tagacggtga tcaggagctg cggctgatcc gtaacctgat cgccgccctg
     3841 cctgaacaaa ccgacctcgg cttgcaggcc ggcctgcgtt atcagctgac tacctacggc
     3901 atctggggtt acgccatgct cagcagccag accttccgcc aggccgccga ccttggcctg
     3961 cgctacctgg acctgacctt tgcctttaac cgcattacct tgcgcgaaga cgccagccac
     4021 gcgcatctgc agctggatgg cagccacttg ccggaccagg tacgcgattt tctgctgctg
     4081 cgcgataccg tggcggtgat ggtgattcag cgcgagctaa ctggcgccaa gattccccta
     4141 agccgggtgc aactgagcat gccggcgccg gctgatccga ggcgctttat cgagcaattt
     4201 ggcctgctgc cggaattcgc ctgcgcccag aaccgcatca gcttccctcg ccacctgctc
     4261 gacctgcccc tgccccgcgc caatccgcac aacgcgcaac tctgcgagct gcaatgccag
     4321 gctctgctgg ccaaacggca agtgcgcagc ggcctggccg gacagattcg tgaccgtttg
     4381 ctgagcacac ccggccggct gccagatatg gagctgatcg caaacgagtt gaatatcagc
     4441 agccgcacct tgcgccgccg cctggaagac cagggcagca gctttcgcca gttgcaggaa
     4501 gaagtacgcc aggccctggc cgaagaactg ctggccatcg ccagcctgag cctggaggag
     4561 attgccgagc gcctgggtta tagcgaggtg tcgaatttcc tgcatgcgtt caaacgctgg
     4621 aaaggcctca cgcctgggca atatcgacaa gagctggcct gaaagtgcga ctgacaagtc
     4681 gtcggcgcag atggcacttt gctacttcat ccgccacatt ggcagtttcc atcctcaagc
     4741 tggcgggcat ttgcgccgat actgctgaat aaccaactga ctggagtttt catgcgacag
     4801 ggtttccttg ggcagttcct gccacatgat ttgaccgacc agaccagcat cctgttggct
     4861 cgtaccctgg ccttcagcgg cttgtgcagc gtggttatcg gcctgtacag ctgcatcaaa
     4921 tggggccgca tgggcaacga tgcgctgatg tacggctcat tgctgctggt gctgggcatg
     4981 ccgctgatgc tgctggtgtt gcgcagcgcc gtgctgccgc tggccgtcgc cgcgaacatg
     5041 gcgctggcct gcatggccag ctactcaatg ttgctgatct accaactggg cggcctgcat
     5101 tcggcgcata tctactggcc ggcggtaatt atcgtcttcg cctacttgct ggccggcgcc
     5161 cgcagcgcga cggtctggag cctggtgcag gtgctgtttg tgttctggct gatccgcctg
     5221 gagcgcagcg gtgccagcct gccggtattc gaactgagcc cacgggatgc cgccgtcaac
     5281 acctactcgg gctacatcct gccggtgctc accgtgtggc tggcacagtg gtacagcgcg
     5341 caactgcgta accaggcgct ggccgatgcc gagctggcgc ttagcgagtc ccgcgagttt
     5401 ggccagcgcg ccgcgcgcaa tcaggagcag ctcggtggca tcgttgatga cgtgcgcagc
     5461 accgccagcg atctgctgca gatggcgggc caactgcagc agaccctcgg cggtattcgc
     5521 cagcgctgcc agagcatcga caccgatgtg cagcaacagg ccgatgccat gcaccagctg
     5581 gatctggccc tgcatgaagt gctcgacagc ctcgcctcca gcaccgacca catgcagcag
     5641 ctcagccagg acacccagca cagcagcagc caggtgaatg cctgcgcaca gcgcatggaa
     5701 gacgcccagg ccagcatgca ggcgattcag cagagcaacc agcgcatcgc cgaatccatg
     5761 cagatgatca gcgccatcgc ccaacagacc aacctgctgg cgcttaacgc agccatcgaa
     5821 gcggcccgcg ccggcgagca tggccgcggc tttgccgtgg ttgccgatga ggtgcgcaac
     5881 ctctcacggc gcagcaacga aaccgccgac accgtacaga acgtactggg ccaatcgcag
     5941 caaatcatcg acaccggcgc gacccaggtc agtgaggtcg gcaccgcgct aagcagcaac
     6001 gccgggctga ccgacaacct ctccagctcg attctgctgc acaaccaggc gcttacccgc
     6061 gcgcatcaac agctgctgca cgtacgcgac aacagcgccg cacagcgtga tgccagccaa
     6121 cgccaacgcg ccgccagcgc cgaactgctg gatgcccagg agtcgctggt gcaattgggt
     6181 gagcaactag agcagctgtc gcatcagctg catcagcggg tggctagcgc ttagctatgc
     6241 aggaccctct accacatcag ggagagggta aaactccagt ttgaaagtca gttctgcgct
     6301 gacagccagc cgagctgcgg ttattgttcg gccatgagcc aatcgaccaa cagcctgtta
     6361 ctgagctacc aaccgccctg gcagtggcaa cagttccacg cgcactttgc cctgcgcgcc
     6421 ctggctgggg tggaaagcct gcagccgcac agttacgcac gcagcgtgca catcgatggc
     6481 caacgtggct ggttccgcgt gcaggcgctg gctgatcagc acgcacttca gctgcagtac
     6541 agccaatcgc tgcagccctg cctggcgaca cttaccgcgc gggtgcgcaa gatgttcgat
     6601 ctggactgcc agccgcagca gatcagtacg cactttgcca acgatgcgca gctcgggccg
     6661 ctagttgcgc gcaatcctgg actgcgtttg ccgactgctt tcgaaccgtt cgagcaggcc
     6721 gtgcgcgcca ttgttggtca gcaggtcacg gtcaaggcgg cagtgaccat caccggccgc
     6781 ctggttacgc gcctgggcga ggcgctgcag tttgccgatg cgcccgatat tcaacggctg
     6841 ttccctagcc cgaccgctat cgccgaagcc aatctggatg gcatcggcat gcccggcaag
     6901 cgcgtgcaga ccttgcaaca cttcgccgcg cagctagcca gcggcgacct gcgcctggat
     6961 ctgcaggccg gttacaccgc ctttgtcgag cagctgtgca ccctgcccgg cataggccca
     7021 tggaccgccg aatatattgc gctgcgcgcc tttggcgagg tcgatgcctt tcccgccagt
     7081 gacctgggcc tgctcaaagc gccggtgtgg ggtgcatctg ggattgatgc caaacagctc
     7141 aaggcccgcg ccgaagcctg gcgaccttgg cgcgcctatg ccgccgcgca cctgtggcac
     7201 aactattcag agggctgaat cgatgttcta ccgctaccac gacagcccga tcggccgtct
     7261 gctgttggcc gctgacgaaa ccggcctgca acaactgctg atggatatcg atgacaagcc
     7321 ctggcagatc ggcgaacact ggcgcagcgc cgccgatgaa ctggatgaag tatgccgaca
     7381 gctggatgag tatttcgcca ggcgtcggca gcgcttcgaa gtgcgcctgg cgccgcaagg
     7441 cacggcgttt cagcaagccg tttggcaagc gctgcagacc atcccatttg gccaaaccag
     7501 ctgctacagc gccctcgccg ctgagatcgc gcggcccaag gcagtacgcg ccgttggcgc
     7561 cgccaatggc gccaacccga ttgccatcat cattccctgc cacagggtaa tcggccgtga
     7621 cggcagcctt accggttatg ccggcggcct gccgcgcaaa gcgctgttgc tcaaactgga
     7681 aggggcgcag ctaccggaac agcggctgct gagtatttaa cctgcgcagc gattcagggc
     7741 tgaactcgcg ccagtaagcg cgccggagcc tctagcggta acgccagaat cagcccttcg
     7801 ccagacgccg ggtagatgcc ggtcagcgcg gtgatattga cctgatggga taccagcacc
     7861 agcgacgcac cacggggttg cgcattaatc tgctcgatca gcgcggcggt ttgctcacgg
     7921 gcggtgtggg gtgtttgaaa gaatgaatcc agagctggca gcgcttcgac tgcgcccaag
     7981 cccatctcac gagctgtgtc ctgcgcgcgg caccagcggc tgctgagcag acgcggctcg
     8041 atccactggc tacccaacaa ttgaccccaa cgctgcgctt gctcgcgacc aagctgattg
     8101 agattgcgct gagtgcggca atcactcagc tggaaattcg ccggatcacc cgtgccgggc
     8161 gccatggcat ggcgcaacag cagcacggcg cggccttcac gcaacgcggt ccaggcgagc
     8221 ttatcgctgg cctgaaccgc gccgctcagc aggcaaagcg ccaataacca cgcgccggcc
     8281 cgccgcatgg ctagaacttg accgtgccac gcggccccat cagcgcccag atgatcaggc
     8341 cgatcactgg cagcaagacg atcagcaata tccacaacac cttgatgccc gtctcggcgt
     8401 tgcttttgat aacgctgaga atcgcccaga tatccagcgc cagaatcacc agccccagta
     8461 aaccgttgaa tgttgaaccc ataatctgac gctcctatgc gaaggtttcg cctccgtagg
     8521 atagccgctc cctacaagag ttcagcagtt tgctagttca gcagtcatcc agtccggcaa
     8581 acgcggcggc aaaatcaccc tccacaggaa caaaacccgg cacctgcggg ctatcgaaca
     8641 gacgcaacac ggcaaagcgc ggatgctcgc cgccatcgaa ccaatgcgcc gtgccggcgg
     8701 gcaacagcag caggtcattc ttctcgcaca acaggctgta gacgtactca ccaatatgca
     8761 gcgccagcag gccacgcccg gccaggtagt aatgcaggtg agccggctta caagtttgtt
     8821 cctggcgcag agcacgggcc agttcactgc cctcaccgcg ctcatcacac aggctcagga
     8881 cctcggcact gacgtagcca tgcgtgacca tcaattgggc aatctgtaca cggcaggcat
     8941 ccatcaactc atagttggca gtgccggcgg taactggctg ctctaccggc acctgactga
     9001 actgcacgcc gacggcggcc agggtgctgg caatgtcttc gacgtgggtc agcagcttgt
     9061 tcgggtactc aggcgaggac tggtggtaga cggtcaggct gctcatggtc agggctctcg
     9121 tacggttcaa ttcaggtgtt taattcaaca gatcaagtct agggccgctc atcacgcata
     9181 taacgcgcgg tgatcactgc agccgccagg gcaatcaggc tggcgatggc aaaagtccag
     9241 gcggcgccga ggctgtgcca gctgtaaccg gaatacagcg cgcccaacgc accacctaca
     9301 cccgccaatg cggcgtacag ggcctggccc tggccctgct ggcggtcggc aaagctgcgt
     9361 tgaacaaaat gcatggccgc cgcatggaag ctgccaaaag tcgccgcatg catcagctga
     9421 gcgaacagca gtaccggcaa gtgcccagcc agattaccca gcaaaagcca gcgcacggca
     9481 gtaatcagaa agctcgccag cagcacggta cgcagggaat aacgcgccag caaccgggcc
     9541 atcaccataa acagcacgat ctcagccacc acgcccagcg cccacagctg gccgatcacc
     9601 ccgcgcgaat agccgagtgc ttccaggtgc agactgagaa aggtgtagta cgggccgttg
     9661 ctcaactgca tcagcccggc actgaggtag aaggcgagaa tccccggtcg ccgcaactgt
     9721 tgcagaaaac cgccctgttc cagggtattg gggcgcatct gcggctgcgc attgggcacc
     9781 caccagctgc tcaggacgat gccgctcatg atcaggatca gcgcccaggg ataggcgtcc
     9841 aggctaagca cttcgaacag cttgcccaga ccgaccaccg cgacgataaa cccaatgcta
     9901 ccccacaggc gaatctggct gtagcgcgcg gcctgctcct tgagatgggc aaaggtgatg
     9961 acttcgaact gcggcagcac cgcgtgccag aagaacgcat gcagcgccat gatcagcgcc
    10021 agccaggcgt agctctggct gataaagatg ccggcaaagc acagcagcgt gcagagcgca
    10081 ccaaagcgca caatggccaa gcgctggccc gtgtgatcgc ccagccaacc ccagatattc
    10141 ggcgcaatgc agcgcatcag catgggaatg gcgatcagct caccgatccg cgcagcggaa
    10201 aaccccaggt gatcgaaata cagcgcgaga aagggcgcgg tggcgccgag cagcgagaaa
    10261 tagaagaaat agaaaccgga cagacgccag tacggcaaaa ccggactcat gctgagcaca
    10321 aacccggcag cgtgctgcgg agcgcacgtg gccgcgcagc agcgctgatc acagctgtgg
    10381 cagcaccggc gtactgaccc gcacatcggc attctgcgcg cggtggcgca gcaggtggtc
    10441 catcagcacg atagccatca tcgcctcggc aatcggcgtg gcgcggatgc ccacgcacgg
    10501 gtcatgacgg cccttggtga tgatatccac cgggttgccg tgaacatcga tcgagcgccc
    10561 cggcgtggtg atgctcgagg tcggcttgag cgccaggtgc gcaacaatcg gctgaccact
    10621 ggaaagcccg ccgagaatgc cgccggcatt attcgagaga aaaccttcag gtgtcatctc
    10681 gtcacggtgc tcggtgccgc gctgcgccac gctggcgaaa cccgcaccga tttccacacc
    10741 cttgaccgca ttgatgctca tcagcgcatg ggccagttcg gcgtccaggc ggtcgaaaat
    10801 cggctcgccc aggccgggca taacgccctc ggcaaccacg gtgatcttcg ccccaaccga
    10861 atcctgatca cggcgcagct ggtccatata ggcttccagc tccggcactt tgtcaggatc
    10921 ggggctgaag aaggcgttct gttcgacgct gtcccaggtc ttgaacggaa tttcaatcgg
    10981 gccgagctgg ctcatgtagc cgcgaatcac gatgccctga gtcgccaggt atttcttggc
    11041 gatggcacca gcggccacac gcatggcggt ctcgcgcgcc gaactgcggc caccaccgcg
    11101 gtagtcacgc acgccgtact tgtggtgata ggtgtaatcg gcgtgggccg ggcggaagga
    11161 gtccttgatc gccgagtagt ctttggactt ctggtcggta ttgcggatca gcaggccaat
    11221 cgagcaaccg gtggtcttgc cctcaaacac cccggcaagg atttccacct catcggcttc
    11281 ctggcgctgg gtggtatgac ggctggtgcc tggtttgcgc cgatcgaggt cacgctgcag
    11341 gtcttccagg ttaatctcca gccccggcgg gcagccatcg acaatggcga ccaacgccgg
    11401 gccatggctt tcgccagcgg tggtgacagt gaacagcttg ccgtaggtat tgccggacat
    11461 gtgcaaaacg ctccgcgaaa atcagctccg aggctacgcc ag
//
LOCUS       DASRUM010000279         5535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_657786, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000279 DASRUM010000000
VERSION     DASRUM010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657786"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..98)
                     /locus_tag="VFF22_18660"
     CDS             complement(<1..98)
                     /locus_tag="VFF22_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18876.1"
                     /translation="MMRSHYCGQLNESLDGQEVTLCGWVHRRRDHGG"
     gene            391..1509
                     /locus_tag="VFF22_18665"
     CDS             391..1509
                     /locus_tag="VFF22_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZX18877.1"
                     /translation="MATPNLAEIPSDLLADFRLESSEQFQRCEQLLIELEHAPDDRER
                     LRELFRLVHTLKGNLGYIGLDTLMSVPQAMEDVLSCLREGQLHFDSLLGDILLLTLDH
                     LKELIQHALGGPPAQLNTTQSKALSQALSALALAPAAQQTGIRSALLQQLDPSSRPPA
                     AIAADEPAAADLLRRYGIEPDADLLFFVDLMQASERRSHFWQGRGLRQLDLALAINQL
                     GGNPEQPEQLAAAICLHDLGMAFLPLEMLHKEAHFNREERRQMQSHPRLAYDLLKRMP
                     RWDAAAEITLQHQENADGSGYPKGLKEVETSPGALIINIVDTFDARTHERAHQTLNKR
                     PLLRAILEINNLAGRQFSAHWVELFNQTLQQHPELAHR"
     gene            1659..2129
                     /locus_tag="VFF22_18670"
     CDS             1659..2129
                     /locus_tag="VFF22_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HZX18878.1"
                     /translation="MEINIGIAEQDRAAIAEGLSRLLADTYTLYLKTHNFHWNVTGPM
                     FNTLHLMFEGQYTELSLAVDQIAERIRALGFPAPGTYAAYARLSSIKEEEGVPSAQDM
                     IKQLVQGQEAVVRTARGIFPLLDKVSDEPTADLLTQRMQVHEKTAWMLRSLLAA"
     gene            2248..3315
                     /locus_tag="VFF22_18675"
     CDS             2248..3315
                     /locus_tag="VFF22_18675"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZX18879.1"
                     /translation="MNKPAKAPSILELYPEETRDAAALLKKAVPLMMRHDIPPNPVHY
                     ALWYTYSKGLEPELNRRLEKTVADFDSFPPETAAKLFRDYIIHGELEEARAGQQQVIE
                     LVDDIEGNVSRSIIGSRNYQASLEHGLAALQEPIIDDLPNVLNELQQSTQLMQDLQDK
                     FLYRLNAAQQEIKSLRSQLERAHIAATLDSLTQVFNRNAFSRLLEQTLSTEHDGVALV
                     MLDIDHFKQFNDQYGHPLGDRVLQHVGQLLRDLLPPRAMAARYGGEEFCVILRDCFDN
                     ATAYAFAEQLRLKIQSLRIKVRRTDEVLDSITASFGFALAEPGDTFEALLTRADDALY
                     QAKRDGRNQVSPNPSETALSA"
     gene            3482..3802
                     /locus_tag="VFF22_18680"
                     /pseudo
     CDS             3482..3802
                     /locus_tag="VFF22_18680"
                     /inference="COORDINATES: protein motif:HMM:NF041604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein membrane
                     protein"
     gene            3860..4084
                     /locus_tag="VFF22_18685"
     CDS             3860..4084
                     /locus_tag="VFF22_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZX18880.1"
                     /translation="MGDRETGTVKWFNTSKGFGFISRDSGDDIFVHFRAIRGEGHRVL
                     VEGQRVEFAVMQRDKGLQAEDVIAALPARR"
     gene            complement(4139..4345)
                     /locus_tag="VFF22_18690"
     CDS             complement(4139..4345)
                     /locus_tag="VFF22_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HZX18881.1"
                     /translation="MEIEERINDLQSRLAFQDDTIQALNDALVAQQRLLERLQLQVAA
                     LIKRQDEVSSQFGMAEDEAPPPHY"
     gene            complement(4349..4774)
                     /locus_tag="VFF22_18695"
     CDS             complement(4349..4774)
                     /locus_tag="VFF22_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003227542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HZX18882.1"
                     /translation="MFVLDSRLQQDTVWIGDYPLCSLLLMNDVQYPWFILVPRREDVS
                     ELFQLDAADQLQLWRETNALAEVLKDCFAADKMNVATLGNVVSQLHMHVIVRRREDAA
                     WPAPVWGRYPAQAYTAEQLAALKARLRLVLTGDFCFAEN"
BASE COUNT         1394 a         1791 c         1327 g         1023 t
ORIGIN      
        1 ccgccatggt cacgacggcg atgtacccaa ccgcaaaggg tgacttcctg gccgtccagg
       61 ctctcgttca gttggccgca ataatggctg cgcatcatgg tggtggtcgc ttctcttgag
      121 tcttgaattc gttgggccgc agtgactgcg acgctcgttg gccggacaac cggctggcgc
      181 aggcgccatt ttccgcaata gtgctgcctt taaggggcgc gaaacgatca gcggcgccta
      241 cgcagccaac tgttcgcccg acaaaacgcg gcaaagcgcg ggattatata tggttaagcc
      301 gccacttgca gccgccgccc cacgcgcttt tgccagccct tcctatactc aggcatgcag
      361 cactctgacc gtcgaatagt ggagaggctt atggccacgc cgaacctggc tgaaatcccc
      421 agcgatttgc tggcagactt tcgcctggaa tccagcgaac aattccagcg ttgcgagcaa
      481 ttgctgattg agcttgaaca cgcccctgat gaccgcgaac gcctgcgcga gctgtttcgc
      541 ctggtgcata cgctcaaagg caacctgggt tatatcggcc tcgacacgct gatgagcgtg
      601 ccgcaggcca tggaagatgt gctgtcgtgc ctgcgcgaag gccaattgca tttcgatagc
      661 ctgctgggcg atatccttct gttgaccctc gaccatctga aagagctgat ccagcacgca
      721 ctcggcggcc cgccagcgca actcaataca actcagagca aagcgcttag ccaggcactc
      781 agcgctctgg cactggcgcc agcggcgcaa caaaccggga ttcgcagcgc actgctgcaa
      841 cagctcgacc ccagctcgcg gccgccagca gccattgctg cagacgaacc cgctgccgcc
      901 gatttactca gacgctacgg cattgagcct gatgctgacc tgctgttctt cgtcgacctg
      961 atgcaagcca gcgagcgccg ctcacatttc tggcaaggcc gtggcctgcg tcagctagac
     1021 ctggccctgg cgatcaacca gctgggcggc aacccggaac aacccgaaca actggcggca
     1081 gccatctgcc tgcacgacct gggcatggcc ttcctaccat tggagatgct gcataaagaa
     1141 gcgcatttca atcgcgaaga acgtaggcag atgcaatcgc acccacgcct agcctacgac
     1201 ctgctcaagc gcatgccgcg ctgggatgcc gctgcagaga taaccctgca gcatcaggag
     1261 aacgccgacg gtagcggcta ccccaaaggc ctgaaagagg tcgaaaccag tcccggcgcc
     1321 ctgattatca atatcgtcga caccttcgac gcccgcaccc atgagcgcgc ccatcaaacc
     1381 ctgaataaac gtccgctgct acgcgccatt ctggaaatca acaacctcgc aggaaggcag
     1441 ttcagcgcgc actgggtcga gctgtttaat cagacgctgc aacaacaccc cgaactggcg
     1501 catcgctaag ccctagaaaa attgaaccca gacccagcaa tagcctccaa ccatcacttt
     1561 cattgatata aaccacgata aagctcggca acacccatgg taggctctgc ccatcacgcg
     1621 ggcagccaag ccccgagcca cctgagagga gaaatggtat ggaaatcaat atcggtattg
     1681 ccgaacaaga ccgcgccgcc atcgccgaag gcctgtcgcg cctgctcgca gacacctaca
     1741 ccctgtacct gaaaacccac aacttccact ggaacgtcac cggcccgatg ttcaacaccc
     1801 tgcacctgat gtttgaaggt caatacaccg aactgtcgct ggccgtcgac cagattgccg
     1861 agcgcattcg cgccttgggc ttcccggccc caggcaccta tgccgcgtac gcccgcctct
     1921 cgtctattaa ggaagaggaa ggcgtgccga gcgcccagga catgatcaaa caactggttc
     1981 aaggccagga agcggttgta cgcaccgccc gcggcatctt cccgctgctg gacaaagtca
     2041 gcgacgagcc cacagcggac ctgctgaccc agcgcatgca agtgcatgag aaaaccgcct
     2101 ggatgctgcg cagcctgctt gccgcctgag taatggataa ataggtaaaa acaagcaaaa
     2161 ggcccgtatc tacgggcctt ttgctaggcg cttgcccagc agctgttcta tgctttggtc
     2221 aattgcaact tggccacagt gacgtgcatg aacaaacctg ctaaagctcc cagcatcctg
     2281 gaactgtacc ccgaggaaac ccgcgacgct gcggcgcttc tgaagaaggc ggtaccgtta
     2341 atgatgcgcc acgatattcc gcccaacccg gtgcattacg ccctctggta cacctacagc
     2401 aaaggcctgg agcctgaact caatcgtcgc ctggagaaaa ccgtcgctga cttcgacagt
     2461 ttcccaccgg aaaccgcagc caaactgttt cgcgactaca tcatccatgg cgagctcgaa
     2521 gaagcccgtg ccggccaaca gcaggtcatc gagctggtcg acgacatcga aggcaatgtg
     2581 tcacgcagca tcatcggcag ccgcaactac caggcaagcc tggagcatgg ccttgccgca
     2641 ctgcaggaac cgatcatcga tgatctgccg aatgtcctga acgagctgca acaaagcacg
     2701 caactgatgc aggacctgca ggacaagttc ctctatcgcc tgaatgccgc tcagcaagaa
     2761 atcaagagcc tacgcagcca actcgagcgc gcgcacatcg ccgcaacatt ggacagcctg
     2821 acccaggtct tcaaccgtaa cgccttcagc agactgctgg agcaaaccct gagcaccgaa
     2881 catgacggcg tcgcactggt gatgctggat atcgaccact tcaaacaatt caacgaccaa
     2941 tacggccacc ccttgggtga tcgcgtactg cagcacgttg gtcaattgtt gcgcgaccta
     3001 ctgcccccac gggctatggc cgcacgctat ggcggcgaag agttctgcgt catcctgcgc
     3061 gactgctttg acaacgcaac ggcctacgct tttgccgaac agctgcgcct gaaaattcaa
     3121 tcactgcgca tcaaagtgcg ccgaactgac gaagttctcg acagcattac cgcatctttc
     3181 ggcttcgctc ttgcagaacc aggcgacacc ttcgaagcgc tgctcacccg cgccgatgac
     3241 gcgctttatc aggctaagcg cgacggtcgc aatcaagtta gccccaaccc aagcgaaaca
     3301 gcactcagtg cttgattgag aaccccacct ctttgctgtt aaatacgccc cgtgccgcta
     3361 aaccgcatct atggtttggc gcacaggctg gtctcacgcg cttttactcc tcccctgcaa
     3421 cgcccaatac gcgcagccag cccttcctgt tgactcttta aattaagcga gctcattgaa
     3481 catgttgaaa attgtccacc ttctgacggg caccctcgcc ctgctacttt cttttgctcc
     3541 acactttggc gacacaccac agtcactctt agaacaaccc agcgcactgt gccttgccct
     3601 gttcggcctg cttaacctgc tgcttgcccc acaactaccc atctggcacg ccggcctgcg
     3661 ccatcaagca caaaacctgg tttccgccct cctggtactc ggcaccctgc tacaagcctt
     3721 gatcctgcta gccccgttac caaaaattgc cgagcaaccg gccatcctat tcagcctgct
     3781 gatcatcgct gccgccgtag ccgtacacct ggcggccaac ctgcgccttc aatcgcgcaa
     3841 cacctcaagt tcgcaggatc tgggcgaccg cgaaacaggt accgttaagt ggttcaacac
     3901 ctcgaagggt ttcggcttta tctcccgcga ctctggcgac gatattttcg tacactttcg
     3961 cgccattcgc ggtgaaggcc accgcgtact ggtcgaaggc cagcgggtcg agttcgccgt
     4021 aatgcaacgc gacaaaggcc tgcaagccga agacgtcatc gccgcgctgc ctgcgcgacg
     4081 ctaagcgcac aaacgcaaaa ggcccgcaca ctgcgggcct tttgcgtttt atagctaatc
     4141 agtaatgggg cggcggcgcc tcatcttcag ccataccgaa ctgggaactc acttcatcct
     4201 gacgtttgat caacgcagcc acctgcagct gcaagcgctc tagaagccgc tgctgcgcca
     4261 ccagcgcatc attgagtgcc tgaatagtgt cgtcctgaaa agccagtcgg ctttgcaaat
     4321 cgttgatgcg ttcttctatt tccaccagtc aattctcagc aaaacaaaaa tccccggtca
     4381 aaaccaagcg taaacgggct ttcagcgccg ccaactgctc ggcagtatat gcctgcgcgg
     4441 gataacgccc ccacacaggc gcaggccaag ccgcatcctc gcgacgccgc acgatgacat
     4501 gcatatgcag ctgactaacg acattaccca atgtcgcgac attcatcttg tcagccgcaa
     4561 aacaatcctt gagcacttca gcaagagcgt tggtttctcg ccagagctgc aactgatcag
     4621 ccgcatccaa ctgaaacaac tcgctgacat cttcacgacg cggcaccagg ataaaccaag
     4681 ggtactgcac atcattcatc aacagcaggc tacatagcgg gtaatcccct atccagacag
     4741 tgtcctgctg caaacgcgaa tccagaacaa acataacgat ctacctttga taaagttcac
     4801 caactcgggt ttgacataaa tcgaacaagc caaacgtcgg cgcccgaaac ctaatcagcc
     4861 aactgactgc caaatcaacg ccacaaaaca ccagcagcac aggatacgcc gaagccgaaa
     4921 acacggcacc cgcgatcaac ccgccaccac gcaccaatag acagcactca acaaagccat
     4981 accaagcagg cgcaccaaaa ctgttccctt cggtaatccc cacccctccg ccaaatgcca
     5041 cgcaacgctc acatgacggc aaacaactga aaacagagca gaaagcgacc gcaaaaacac
     5101 ccatctcggc ctataaaaat agaaaaaaac gctgaaaaac acaccacaaa ccaatgcaaa
     5161 ccccgaaaac catgaccaca cagatcaaat cagggataaa aaattgcctt tccggatttt
     5221 gtgcacgact attgctaagc aatccgtgac gaatcgaact gcaccgtttg gctgccgaag
     5281 gtgccagcga gcaagcgcaa aaatccgtca gcgttgactt ccagcacgcc agtagcatct
     5341 acaaaaatgc gacatgattc ataagctttt gcgacacagc catgaagaaa tcgcaatgaa
     5401 tagattgcaa ctatcgccaa cattggcagc gtgctataag ttaccgccga cacaaaaaga
     5461 aagagtcgtc catataacaa ccagccggac ggcgcaactt ttctaaacca aaggagcaat
     5521 cacgatgcaa gtgat
//
LOCUS       DASRUM010000280        31115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_315651, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000280 DASRUM010000000
VERSION     DASRUM010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 31115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31115
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315651"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..945)
                     /locus_tag="VFF22_18700"
     CDS             complement(238..945)
                     /locus_tag="VFF22_18700"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HZX18883.1"
                     /translation="MLQVSVYRYNPEKDAAPFMQDFQVDTGGKDIMVLDVLALIKEQD
                     EGFSYRRSCREGVCGSDGMNINGKNGLACITPISAAGLKGGKLVIRPLPGLPVIRDLA
                     VDMSIFYKQYEKVQPYLQNNTPAPAIERLQSPEEREKLDGLYECILCACCSTSCPSFW
                     WNPDKFLGPAALLQAYRFLADSRDTQTEQRLASLDDPFSVFRCRGIMNCVNVCPKGLN
                     PTKAIGHVRNMLLQSGT"
     gene            complement(972..2744)
                     /gene="sdhA"
                     /locus_tag="VFF22_18705"
     CDS             complement(972..2744)
                     /gene="sdhA"
                     /locus_tag="VFF22_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HZX18884.1"
                     /translation="MTSIRTLSYDAIIVGGGGAGMRAALQLAQGGHKTAVVTKVFPTR
                     SHTVSAQGGITCAIASADPNDDWRWHMYDTVKGSDYIGDQDAIEYMCSVGPEAVFELE
                     HMGLPFSRTEQGRIYQRPFGGQSKGPNNPTQAARTCAAADRTGHALLHTLYQANLKAG
                     TSFLNEWYAVDLVKNQDGAVVGTIAICIETGETVYIRSKAVVLATGGAGRIYASTTNA
                     LINTGDGVGMALRAGVPVQDIEMWQFHPTGIAGAGVLVTEGCRGEGGYLINAHGERFM
                     ERYAPNAKDLAGRDVVARSMVKEVLAGNGCGPDKDHVLLKLDHLGEDVLHSRLPGICE
                     LSKTFAHVDPVVAPVPVIPTCHYMMGGVPTNIHGQAIMQDANGNDKIIEGLFAVGEVA
                     CVSVHGANRLGGNSLLDLVVFGRAAGIHLEKALKEGVEVRGASETDIELSLKRLAGVN
                     ERTTGEDVAPLRKELQNCMQNYFGVFRTGEYMQKGIAQLADLRERIATVKIADKSQAF
                     NTARIEALELQNLLEVAEATAIAAEVRKESRGAHAREDFEERDDENWLCHSLYIPGEK
                     RVSKRSVNFSPKTVPAFEPKVRTY"
     gene            complement(2748..3116)
                     /gene="sdhD"
                     /locus_tag="VFF22_18710"
     CDS             complement(2748..3116)
                     /gene="sdhD"
                     /locus_tag="VFF22_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HZX18885.1"
                     /translation="MVTNVTNFSRSGLYDWMAQRVSAVVLALYVLFLLGFILCNPGLG
                     YAEWQALFSNNAMRIFSLLTLVALSVHAWVGMWTISTDYLTPMALGKWATAVRFLFQA
                     ACGVAMFAMFAWGVQIFWGN"
     gene            complement(3110..3484)
                     /gene="sdhC"
                     /locus_tag="VFF22_18715"
     CDS             complement(3110..3484)
                     /gene="sdhC"
                     /locus_tag="VFF22_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007244768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HZX18886.1"
                     /translation="MNSQRPVNLDLRTIKLPITAYTSILHRISGVILFVGIAILLYGL
                     DKSLSSAEGFAQVQECLTSPLAKFVIWGLLSALLYHLVAGVRHLIMDMGIGESLEGGK
                     LGSKIVLVVSTIVIVLLGVWIW"
     gene            3847..5121
                     /gene="gltA"
                     /locus_tag="VFF22_18720"
     CDS             3847..5121
                     /gene="gltA"
                     /locus_tag="VFF22_18720"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HZX18887.1"
                     /translation="MADKKAQLIIEGAAPVELPILTGTVGPDVIDVRGLTATGRFTFD
                     PGFMSTASCESKITYIDGDQGILLHRGYPIEQLAEQSDYLETCYLLLNGELPNAEEKA
                     KFVSTIKNHTMVHEQLKTFFNGFRRDAHPMAIMCGVVGALSAFYHDSLDINNPHHREV
                     SAMRLIAKMPTIAAMTYKYSMGQPMMYPRNDLNYAENFLHMMFNTPCEIKPISPVLAK
                     AMDKIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIAALWGPAHGGANEAVLSML
                     DEIGDVSNIDKFIAKAKDKNDPFKLMGFGHRVYKNRDPRATVMKQTCDEVLKELGIKN
                     DPQLELAMRLEEIALTDPYFKERNLYPNVDFYSGIILKAIGIPTSMFTVIFALARTVG
                     WISHWKEMLSSPYKIGRPRQLYTGYPQRDMKK"
     gene            complement(5188..5790)
                     /locus_tag="VFF22_18725"
     CDS             complement(5188..5790)
                     /locus_tag="VFF22_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HZX18888.1"
                     /translation="MVRFTGLLLGVAALLAVSGAQAAQWRLVKDEAGIQVYLQQIPGS
                     SFQAFRGVTRIRADMPRLLALQDDVSAACAWIHACSEQKLLKHEGDLSWAYSRFHTPW
                     PVQPRDSILQVTTTRDADGRITRTLHGVADYLPLQQGFVRVSKVEGFWSLTPRDGEIE
                     VIYQVHSEPGGSVPAWLANSFVVDAPFNTLQGLRQLAEKP"
     gene            complement(5827..6087)
                     /locus_tag="VFF22_18730"
     CDS             complement(5827..6087)
                     /locus_tag="VFF22_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZX18889.1"
                     /translation="MNCRAGCGACCIAPSISSPIPGMPAGKPAGVRCVQLDANNYCLI
                     FGQPERPAVCSAFHAAEYVCGSNTADALQLIGWLEQSTAVEV"
     gene            complement(6363..7250)
                     /locus_tag="VFF22_18735"
     CDS             complement(6363..7250)
                     /locus_tag="VFF22_18735"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZX18890.1"
                     /translation="MKVEVDMAKLAFIGLGVMGYPMAGHLARAGHQVCVYNRTAAKAA
                     QWAAEYAGSHAPTPREAATGAEFVMVCVGNDDDLRGVVLGEQGAFAGMQPGAVLVDHT
                     TASAQVARELAEQAAARQLGFLDAPVSGGQAGAQNGALTIMVGGEAATYAKAEPIIQA
                     YARMARLMGEAGSGQLTKMVNQICIAGLVQGLSEALHFAECAGLDAHGAMEVISKGAA
                     QSWQLENRHKTMLAGEFEHGFAVDWMRKDLSILLAEARSNGAQLPVTALVDQFYADVQ
                     GMGGGRWDTSSLFARLHKP"
     gene            7450..8001
                     /locus_tag="VFF22_18740"
     CDS             7450..8001
                     /locus_tag="VFF22_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HZX18891.1"
                     /translation="MQHWLVIDLEATTEEGGWPVTDMEIIEIGASLVNAGGHEIEYFQ
                     RFVRPTRRPHLTHFCRQLTQISQADVDAAAPLSVVWAQFERWLLAHQAQLAGWASWGD
                     YDRRQFEQEWHAHGLESYLSQIPHINLKKRFAEARQLPSPIGLKMALSLAGLQFQGQQ
                     HRALTDARNTARLLPLVLPVQSR"
     gene            8065..8346
                     /locus_tag="VFF22_18745"
     CDS             8065..8346
                     /locus_tag="VFF22_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HZX18892.1"
                     /translation="MFKVNEYFDGTVKSIAFGMVEGPATIGVMAAGEYEFGTNQLEIM
                     HVVAGALTVKLPGSENWETFTSGSKFTVPANSKFQLKVAADTAYLCEYR"
     gene            8419..9291
                     /gene="rhtA"
                     /locus_tag="VFF22_18750"
     CDS             8419..9291
                     /gene="rhtA"
                     /locus_tag="VFF22_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HZX18893.1"
                     /translation="MPRSTQFSLVILPIILLLIAMTSIQSGASLAKGLFPEIGASGTT
                     ALRLSLGALILCILMRPWQAKLTLKSSRSLLAYGLSLGGMNLLFYLSLKTIPLGIAVA
                     LEFTGPLGLALLSSRRLVDFVWIALAVFGLWLLLPTGQSDVPLDPVGMALALAAGLCW
                     ALYIVFGQKAGAEHGKQTVALGTIVAALLVFPIGLWQAGSGLFSVDLLPIALAVAVLS
                     SALPYSLEMVALTRLPARTFSILMSMEPAIAALSGLLFLSERLTLSQWLAISAIILAS
                     AGAAATIKPKAKLL"
     gene            complement(9398..11176)
                     /locus_tag="VFF22_18755"
     CDS             complement(9398..11176)
                     /locus_tag="VFF22_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HZX18894.1"
                     /translation="MLSILSSRQRNALRMAWRFIAPYRGRVFGALLALMFTAAITLSM
                     GQGIKLLVDQGLATQSPAALQQSIGLFFVLVLALAIGTFTRFYLVSWIGERFVADIRK
                     RVFNHLIELHPGFYESNRSSEIQSRLTADTTLLQSVIGSSLSMALRNLIMLVGGSVLL
                     VVTNAKLSGIVLMALPLVVAPILIFGRRVRALSRQSQDRVADVGSYVGEVLGQIKTVQ
                     AYNHQNEDKRRFGLSAEAAFDTARQRIAQRAWLITVVIVLVLGAVGVMLWVGGMDVIA
                     GRISGGELAAFVFYSLIVGSAFGTLSEVIGELQRAAGAAERIAELLQARNEITPPATD
                     ALHLTQPVQGRIELQELRFAYPSRPGCFAVDGIDLQVAAGETLALVGPSGAGKSTLFD
                     LLLRFFDPQQGRILIDGVPIQQLDPRELRSSFALVSQNPALFFGSVEDNIRYGKTAAS
                     LAEVEAAAKAAHAHEFIMKLPDGYQTHLGDAGLGLSGGQRQRLAIARALLVDAPILLL
                     DEATSALDAESEHLIQQALPPLMEGRTTLVIAHRLATVKSADRIAVIEHGKLAAIGTH
                     SELVSSSPLYARLAELQFSNEAEVVG"
     gene            complement(11204..11593)
                     /locus_tag="VFF22_18760"
     CDS             complement(11204..11593)
                     /locus_tag="VFF22_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX-like family protein"
                     /protein_id="HZX18895.1"
                     /translation="MTDPRLRQIAWVARIALALVFIWHGLVPKILWLSPDELAMIQAH
                     NLPAPEWVARIAGLAEIILGLLLLWRRQRWPLLLAGVLLIGLLLDVALFSPHLLLQAF
                     NPLTTNLAALALCWVVWRAETPQSPPH"
     gene            complement(11586..12020)
                     /locus_tag="VFF22_18765"
     CDS             complement(11586..12020)
                     /locus_tag="VFF22_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HZX18896.1"
                     /translation="MQEQTLPPLIQPGERVVLFDGVCKLCNGWAKFLIRHDVEQRFRL
                     ASVQSAEGQALLVWGGLPTDHFDSMALIENGQMLLRSDAVLRIVAQLPKPWRLLAWLR
                     LIPRPIRDWCYDRIALNRYRLLGRYDSCLLPSADHARRFLHD"
     gene            complement(12044..12619)
                     /locus_tag="VFF22_18770"
     CDS             complement(12044..12619)
                     /locus_tag="VFF22_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HZX18897.1"
                     /translation="MRAILLVALSILLSAQAWALQADALVTAARQQVGVTLSYDPAYR
                     RLNYPNGDVPLTTGVCTDVVIRALRQQGLDLQQAVHQDMRANFRLYPKNWGLSRPDSN
                     IDHRRVPNLMTWFKRQGWSLPLGQDAERYRPGDIVTWDLGGGLTHIGIISDRQAGSGV
                     PLVLHNIGRGTQEEDILFSFKITGHYRIPAA"
     gene            complement(12644..13759)
                     /locus_tag="VFF22_18775"
     CDS             complement(12644..13759)
                     /locus_tag="VFF22_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-NAD kinase family protein"
                     /protein_id="HZX18898.1"
                     /translation="MGLFHIGLIINPLAGLGGPAGFKGSDGMAEQALALGVEAKSALR
                     TQTALEVLLPLRGRLEFVTFPGAMGADLLSEMGFAHRVVGELGAGPSSAADTRQAVEL
                     LQDAGVALILFAGGDGTARDICAAVREGQPVLGIPAGVKIQSGVYAISPRAAGELTAR
                     LIEGGLVRLSSGEVRDIDESALREGRVTARWYGELTVPQEGGYVQQVKQGGVESEELV
                     LGDLADWLQDSWETDVRYVFGPGSTLHGLAQNLGVQTTLLGVDVIENGQLLASDVSET
                     QLFELVDGHPARLLVTAIGGQGHIIGRGNQQISPRVLRAIGLENLRVVATKRKLGTLE
                     GRPLLVDSGDVALDDAFPDVVRVWAGYKEELLYPVGK"
     gene            13913..14074
                     /locus_tag="VFF22_18780"
     CDS             13913..14074
                     /locus_tag="VFF22_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010953114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18899.1"
                     /translation="MHSQQQNSQKATTMPIGGFVIDAQGREIPITEEMIQQACSALEE
                     AQQQAGKRK"
     gene            complement(14114..15256)
                     /gene="pdxB"
                     /locus_tag="VFF22_18785"
     CDS             complement(14114..15256)
                     /gene="pdxB"
                     /locus_tag="VFF22_18785"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HZX18900.1"
                     /translation="MQIVADENIPLLDEFFAGFGEIRRLPGRSIDAAAVADADLLLVR
                     SVTQVNRTLLGGSPVKFVGTCTIGTDHLDLNYFQQAGITWASAPGCNARGVVDYVLGS
                     LLVLAEQQGVDLASRTYGVIGAGQVGSRLLKVLRGLGWRVLVCDPPRQAAEGGDYVSL
                     QQVLDECDVISLHTPLERLGEHPTYHLFDAARLAALKPGSWLINASRGAVVDNQALRE
                     LLTQRSDLQVVLDVWEGEPQADVELAGLCRIATPHIAGYSLDGKLRGTAQIYQALCQH
                     LGVAPSIELAQLMPAAWLSELAVDASADPAWALATICRAVYDPRRDDADFRRSLQGDT
                     ATRRAAFDGLRKHYPMRREIDGLEVCVKGDAPALASLVQALGAKLN"
     gene            15425..16804
                     /locus_tag="VFF22_18790"
     CDS             15425..16804
                     /locus_tag="VFF22_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019648299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZX18901.1"
                     /translation="MLVPTRLHRASTELYGLLKLATPIIIAQLANTAMGFVDTVMAGR
                     VSPQDLAAVALGNSIWVPVFLLMTGILLATTPKVAQRFGAGAHTEIGPLVRQALWLAL
                     AVGIAAAVILWNAEIVLSLMNVDPALIEPAMGYLRAVACGFPAVALYHVLRCFSDGLG
                     HTRPSMVIGLCGLALNIPLNYIFIYGKLGLPAMGGVGCGWATGLVMGFMFLSMLWWVK
                     WAPFYQPSKIFSHFEWPQWAVHKRLLSVGLPIGISVFAESSIFAVIALLIGGLGATVV
                     AGHQIALNFSSMVFMIPYSLGMAATVRVGQALGRGEPREARFAAGVSMAAALGYACLS
                     ASLMLLLREEIAQIYTPDRAVIAVATTLLVYSALFQFSDAIQVTAAGALRGYQDTRVT
                     MILTLFAYWGIGLPVGYALGLTDWFGEPSGPSGLWQGLVVGLTCAGGMLAVRLAGSAR
                     KRIRAAKMA"
     gene            16804..17886
                     /locus_tag="VFF22_18795"
     CDS             16804..17886
                     /locus_tag="VFF22_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3080 family protein"
                     /protein_id="HZX18902.1"
                     /translation="MNVRLLLLLAALCLCACTPADDGLALQADYLQRLSNALDVEPAT
                     AFDSSELTRYRLPARRERLIEIAELRISLLDLLVDVRRCPALQQQISLRNNSLGKQLT
                     PSSRLAYEGDLLRALDACSAHLRSQDQQPLRNTLEQLAQEKRAQLPGVFWNALNASPE
                     VERYLRFAEQPLAPNADEDNAALDALAQLAAIGGALPDALPPSAEQLDPLFFALHASD
                     QGGQLISSLASLTHSLNQGSEMLNSRQQRRPLCPTGKATERARIVQNIFVKYYAGSLQ
                     PYLAQVDQRGQVWSSSLRSLSRVMQIPPATQQYLQSLSGREASLWADFQQATARHVHS
                     WQELLNSCGLAPGQSGWKGNTASADQ"
     gene            complement(17844..18299)
                     /locus_tag="VFF22_18800"
     CDS             complement(17844..18299)
                     /locus_tag="VFF22_18800"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HZX18903.1"
                     /translation="MNWYFAYGSNMSLARMQARGLTVLEALPGHLPGYSLCFNKRAAD
                     RAPGRAYANIRHQRDGRVEGVLYRLQDAGEIAKLDHFEGTPIYYSRERLTIVAAHGVQ
                     PAWVYIANPAFRAEGLLPSADYLAHLLAGQALLSEAYWSALAVLPFHPD"
     gene            complement(18299..18550)
                     /gene="tusA"
                     /locus_tag="VFF22_18805"
     CDS             complement(18299..18550)
                     /gene="tusA"
                     /locus_tag="VFF22_18805"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HZX18904.1"
                     /translation="MSLQTTAIPDATLDATGLNCPEPVMMLHNKVRDLQAGGLLKVIA
                     TDPSTRRDIPKFCVFLGHELVEQAEDGATYLYLIRKKLD"
     gene            18696..20237
                     /locus_tag="VFF22_18810"
     CDS             18696..20237
                     /locus_tag="VFF22_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZX18905.1"
                     /translation="MRLVLLLATLLLSGCAARDQLPDFSASAQQDLPARYFDAVIYGR
                     DGTRLSATVFQPALKVGEQAPVIVHTHGWGGWRVTRPNGFYGTQMMSGRAAVKAWQAG
                     YWVISYDQRGWGGSEGDIEMMNPEYEVQDALAVIDWAAANLPRLSMDAPNDPKVGMLG
                     ESYGGAVQLLASATDPRIDAIVPIATWYDLSEALAPDSHMKVGWTSVLLSMGLATGYD
                     LGKFIQPPYLKSVDGRMREPVRAELKAHSLASYCARGQRPQADALLIQGMRDTLFPLN
                     QGLAIRDCLQQGPHDVRLLGMQGGHILPPPMQRWSGLPPFNNEAVLHCDGRAINLYQS
                     IISWYEDKLRGRKGVAASIPDFCVSLDLDQGVALQQLPGNQRIALPATSIRPLSSGLL
                     QPSRFIPLQQVQTSSALIGVPRVHLDNAAQLPTLFAALVVRRADGSSEVLSEQVTPLK
                     GQANTELAAVSAQLQPGDVLGLRISGFSGQYLFNSSWQLSSSTLQGTIEVPQLLPLQP
                     QLARH"
     gene            complement(20240..21295)
                     /gene="rlmM"
                     /locus_tag="VFF22_18815"
     CDS             complement(20240..21295)
                     /gene="rlmM"
                     /locus_tag="VFF22_18815"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HZX18906.1"
                     /translation="MNTLLLHCRPGFENEVCAEIAEHAARLDVAGYAKAKPSTACAEF
                     ICTEDDGAERLMRGVRFNQLIFPRQWARGVFINLPELDRISVLLGHLADFPVFGSLWL
                     EVLDTNDGKELSNFCKKFEAPLRNALTKAGKLVEDAHKPRLLLTFKSGREVFVGMAEA
                     NNSAIWPMGIPRLKFPREAPSRSTLKLEEAWHTFIPREQWDERLSDEMTGVDLGAAPG
                     GWTYQLVKRGMLVTAIDNGPMAESLMDTGLVTHLMVDGFTWKPKQPVDWMVCDIVEKP
                     ARSAALLETWIGEGHCREAVVNLKLPMKQRYAEVRRLLQRLEDGFAERKIKVSIACKQ
                     LYHDREEVTCHLRRLGK"
     gene            21452..24193
                     /gene="acnA"
                     /locus_tag="VFF22_18820"
     CDS             21452..24193
                     /gene="acnA"
                     /locus_tag="VFF22_18820"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010446936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HZX18907.1"
                     /translation="MPSLNSLNSRRSLDIDGQTYHYFSLPEAARALGNLDRLPMSLKV
                     LLENLLRWEDGKTVTGSDLQAMADWLKNRSSEREIQYRPARVLMQDFTGVPAVVDLAA
                     MRDAMAKAGGDPQKINPLSPVDLVIDHSVMVDKFASAAAFGENVAIEMQRNGERYAFL
                     RWGQHAFDNFSVVPPGTGICHQVNLEYLGRTVWTQEEDGQMFAFPDTLVGTDSHTTMI
                     NGLGILGWGVGGIEAEAAMLGQPVSMLIPEVIGFKLTGRLREGITATDLVLTVTQMLR
                     SKGVVGKFVEFYGDGLAELPLADRATIANMAPEYGATCGFFPVDDITLSYLRLSGRPE
                     QTVKLVEAYCKAQGLWREQGQEPLFSDSLSLNMDSVEACLAGPKRPQDKVPLRLVKQT
                     FDDFVGTRLKPPAKEEGRMLSEGGGGTAIGSDESLSEFEYEHEGTVHRLSNGAVVIAA
                     ITSCTNTSNPSVMMAAGLLAKKACEKGLQRKPWVKSSLAPGSKVVTDYFKAADLTRYL
                     DELGFDLVGYGCTTCIGNSGPLLMPIEQAIQQHDLTVASVLSGNRNFEGRVHPLVKTN
                     WLASPPLVVAYALAGSVRLDLSSEPLGIGKDGEPVYLRDIWPSQAEIAEAVQRVDTAM
                     FHKQYAEVFAGDAQWQAIEVPQADTYAWQADSTYIQHPPFFESIGEAPPHIGDIRQAR
                     ILALLGDSVTTDHISPAGNIKADSPAGRYLREQGVAPADFNSYGSRRGNHHVMMRGTF
                     ANIRIRNEMLGGEEGGNTLHVPSGEKLAIYDAAMRYQEQGTPLLVIAGKEYGTGSSRD
                     WAAKGTNLLGIKAVLAESFERIHRSNLVGMGVLPLQFKPGQTRKTLQLSGRETLNICG
                     LDGVELHPSMQLTLDILREDGSRDSIDVLCRIDTLNEVEYFKAGGILHYVLRQLIAG"
     gene            24354..25919
                     /locus_tag="VFF22_18825"
     CDS             24354..25919
                     /locus_tag="VFF22_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HZX18908.1"
                     /translation="MRNNQPVTQRERTFPAQQRLISTTDVKGQITYCNDAFVEISGFS
                     RDELIRAPHNLVRHPDVPAAVFQHMWDTLKKGRPWMGIVKNRCKTGDHYWVNAYVTPV
                     TENNQVVGYESVRVKPTAEQIRRAEALYQRINSGKSAIPSSDRWLPIVQDWLPFILVS
                     QVGFLIGVWLDSSWGFAIAAGLSVPLGLAGLNWQQRGLKRLLRLADQTTSDPLIAQMY
                     TDSRGAQARLEMSILSQEARLKTCLTRLQDTAEQLTQQAKQADTLAHNSSAGLERQRS
                     ETDQVATAINEMAATTLEVASNVARTAIATQDANRLTSAGRSIASETRQAIQRLSQSV
                     GDTGETVTRLAQDSNEIGGVVDVIKGIADQTNLLALNAAIEAARAGEMGRGFAVVADE
                     VRSLAQRTAESTGQIHQLIAKLQRTAEEAVLTMEIGRKQADEGVERVQQADEALAGIS
                     DAVANIADMANQIAAAAEEQSAVADEVNRSITTIAQLADKTAGEAHDTALLSEALTAT
                     AHGQYALVERFNR"
     gene            complement(25964..28243)
                     /locus_tag="VFF22_18830"
     CDS             complement(25964..28243)
                     /locus_tag="VFF22_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid cis/trans isomerase"
                     /protein_id="HZX18909.1"
                     /translation="MVCRILLGVCLLFIGSLQAAEPISYSRDIQPIFTKNCVACHACY
                     DAACQLNLGSGEGVQRGANKATVYNGTRTQAQATTRLFVDAHSPAAWQRKGFTSVLDS
                     QGSQAALMARMLELGHQQPFAANSKLPAELNISIDRVNQCPLPEEFDKFARDNPQAGM
                     PFAVTGLSEADYQTLQTWLEQGAPVDQQALQASTAEAQQISTWEAFMNAPGAREALVS
                     RWLYEHLFLAHLYFASGEPGHFFQMVRSRTPSGQPVDIIATRLPNEDPGNEFYYRLTP
                     VQGVIVHKTHITYGLSAAKLARVKELFFASDWRADAVPGYSLLRRANPFETFAAIPPQ
                     ARYQFMLDNAEYFVRTFIRGPVCRGQIATDVIRDNFWAVFQDPRHDLYITDPEYREQA
                     KDLLAMPGQLDSIGDMIGLWRTYRDKRNEYEALRMQRYADAPAPSWSHIWAGNDNALL
                     SIFRQHDSASVRKGLIGEVPQTLWWLDFPLLERTYYQLVVNFDVFGNVSHQAQTRLYF
                     DLIRNGAEVNFLRLLPAESRQGYLDDWYQSSGRLKIWLDYRAIDTESPSALRLTGNDP
                     KRSFVETLLERYGSLNARPDPINRCTGAHCHRSGLDAELQRAEQSLSRLAAKPAGTLK
                     VIEQLPEATLLRVEMANGQREVYSLLRNRAHTNVAFMLGEELRYQPALDTLTVYPGVL
                     SSYPNFIFNVQVGQAAEFVTALEQARDAVAFEKVVERWGIRRTHPEFWRYFHDMSAYI
                     QQTEPLEAGVLDMNRYENF"
     gene            28407..>31115
                     /gene="metH"
                     /locus_tag="VFF22_18835"
     CDS             28407..>31115
                     /gene="metH"
                     /locus_tag="VFF22_18835"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HZX18910.1"
                     /translation="MSMSLSDRSARLNALQQALKERILILDGGMGTMIQSYKLEEEDY
                     RGTRFADWPSDVKGNNDLLILSRPDVIGAIEKAYLDAGADILETNTFNATQVSQADYG
                     MQALAYELNVEGARLARSVADAKTLETPDRPRFVAGVLGPTSRTCSISPDVNNPGYRN
                     VTFDELVENYVEATRGLIEGGADMILIETIFDTLNAKAAIFAVQQVFEDDGVELPIMI
                     SGTITDASGRTLSGQTTEAFWNSINHAKPISVGLNCALGAKDLRPYLEELSAKAGTHV
                     SAHPNAGLPNAFGEYDETPAEMAAVVEEFAASGFLNIVGGCCGTTPAHIQAIAEAVSK
                     YTPRAIPDIPKACRLSGLEPFTIDRNSLFINVGERTNITGSAKFARLIREDNYTEALE
                     VALQQVEAGAQVIDINMDEGMLDSQKAMVTFLNLIAGEPDISRVPIMIDSSKWEVIEA
                     GLKCIQGKGIVNSISMKEGVEQFKHHAKLCKRYGAAVVVMAFDEAGQADTAARKREIC
                     QRSYDILVNEVGFPPEDIIFDPNIFAVATGIEEHNNYAVDFIEACAYIRDHLPHALSS
                     GGVSNVSFSFRGNNPVREAIHSVFLYYAIQNGLTMGIVNAGQLEIYDEIPKELRDAVE
                     DVVLNRHEGATEALLAIADNYKGDGSVKEVETEEWRTWNVVDRLKHALVKGITSHIIE
                     DTEECRQQCTRPIEVIEGPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKQAVGHLIPF
                     IELEKGDKPEAKGKILMATVKGDVHDIGKNIVGVVLGCNGYDIVDLGVMVPAEKILQT
                     AIAEKCDIIGLSGLITPSLDEMVHVAREMQRQGFTLPLMIGGATTSKAHTAVKIEPKY
                     QNDAVIYVTDASRAVGVATQLLSKELKADFVERTRLDYIEVRERTPN"
BASE COUNT         6495 a        10001 c         9040 g         5579 t
ORIGIN      
        1 cctcggtaag gggacaccac cggcgaagaa accgcatggc tgtcagtcct gaagcagcga
       61 ctggtaagcc actacggatc ccgcagatag tccgggcacc agcccggatg cccctgctgg
      121 tcgtcatgtt tttcaaatta agagccaccg ctttgtgagc agcaaccctg gctaaaacta
      181 cggcgctggg ttgaaagcag cgccgcaggt gttacaggtc tagcaaacaa ccgtgaatca
      241 ggtaccgctt tgcagcaaca tgttgcgtac gtggccaatt gccttggtcg ggttgagtcc
      301 cttagggcaa acgttcacgc aattcataat gccgcggcaa cggaacacac tgaacggatc
      361 atccaacgac gccagacgtt gttcagtctg ggtatcacgg ctgtccgcca ggaaacgata
      421 agcctgcagc agcgcagctg ggccgagaaa cttgtctggg ttccaccaga acgacgggca
      481 gctggtggag cagcaagcgc acaggatgca ctcgtacaga ccgtccagct tctcacgctc
      541 ttccggcgat tgcagacgct cgatggccgg agccggcgtg ttgttctgca ggtatggctg
      601 caccttctcg tactgcttgt agaagatgct catatcaacg gccaagtcac ggatgaccgg
      661 cagaccaggc agcgggcgaa tcaccagctt gccgcctttc aggccagccg ccgagatcgg
      721 cgtaatgcag gccaagccat tcttgccgtt gatgttcatg ccatcggaac cgcacacgcc
      781 ttcacggcag gagcgacgat aggagaaacc ctcgtcctgt tccttgatca gcgccagtac
      841 gtcaagaacc atgatgtcct tgccgccggt gtcgacctgg aaatcctgca taaacggcgc
      901 agcatctttt tccgggttgt aacgataaac gcttacttgc aacagattgg cttgcgacat
      961 atcaggcacc cttaataagt ccggaccttg ggttcaaacg ctggaactgt cttcggcgag
     1021 aagttgaccg aacgcttgct cacgcgcttt tcacctggga tatacaggga gtggcacagc
     1081 cagttttcgt cgtcacgttc ttcgaagtct tcacgagcgt gagcaccacg ggattcttta
     1141 cgaacctcgg cagcgatcgc ggtggcttct gccacttcga gaaggttttg cagctccaat
     1201 gcttcgatac gcgcagtgtt gaatgcctgg ctcttgtccg cgatcttgac agtcgcgatc
     1261 cgctcacgca ggtcggccag ttgggcaatg cccttctgca tgtactcacc ggtgcggaac
     1321 acaccgaagt agttctgcat gcagttttgc aactctttac gcagcggcgc gacgtcttcg
     1381 ccggtggtgc gttcgttgac acccgcaaga cgcttgagcg acagctcaat gtcggtttcg
     1441 ctggcaccac ggacttccac gccttctttc agcgctttct ccaggtgaat accggcggca
     1501 cgaccgaata ccaccaggtc gagcagcgag ttgccaccca gacggttggc accgtggacc
     1561 gatacgcaag ccacttcgcc tacagcgaac aggccttcga tgattttgtc gttgccgttg
     1621 gcatcctgca tgatggcctg gccgtgaatg ttggtcggca cgccgcccat catgtagtgg
     1681 caggtcggaa tcactggaac cggagcaacc accgggtcaa cgtgggcgaa ggtcttcgac
     1741 agctcgcaga tacctggcag acggctgtgc agcacgtctt cgccgaggtg atcgagcttg
     1801 agcagtacgt ggtccttatc cgggccacag ccgttgccgg cgagtacttc tttaaccatc
     1861 gagcgcgcta ctacgtcacg accggccaag tctttggcgt tcggcgcata acgctccatg
     1921 aaacgctcgc catgggcgtt gatcaggtaa ccaccttcac cacggcaacc ttcagtaacc
     1981 agtacaccag cgccggcaat accggtcggg tggaactgcc acatctcgat gtcctgcacc
     2041 ggcacgccgg cacgcagggc catgcccacg ccgtcaccgg tgttgatcag ggcgttggtg
     2101 gtagaggcgt agatacgacc ggcaccgcca gtggccagaa ccacggcctt ggaacggata
     2161 taaacggttt cgccggtttc gatgcagatg gcgatggtgc caactacggc gccgtcctgg
     2221 ttcttcacca gatcgaccgc gtaccactca ttaaggaacg aggtgccagc cttgaggttg
     2281 gcctggtaca gggtgtgcag cagggcgtga ccggtacggt cagcagccgc gcaggtacgc
     2341 gccgcctgag tcgggttgtt cgggcctttg gactgaccgc cgaacggacg ctgatagatg
     2401 cggccctgct cggtacggga gaacggcaga cccatgtgtt ccagttcaaa gacggcttcc
     2461 ggacccacgg aacacatata ttcgatagcg tcctggtcac cgatatagtc ggagcccttg
     2521 acggtgtcgt acatgtgcca gcgccaatcg tcgttcggat cggccgacgc gatggcgcag
     2581 gtgatgccac cctgagcgga aacagtgtgc gaacgggtcg ggaagacctt ggtcacgact
     2641 gcggttttat gaccaccctg agccaactgc agtgcagcgc gcatacccgc gccgccgcca
     2701 cccacgatga tggcgtcata agaaagagtg cgaatgcttg tcatggatca gttaccccaa
     2761 aaaatctgca cgccccaggc gaacattgcg aacatggcaa cgccacacgc agcctggaac
     2821 agaaaacgca cggcagtcgc ccacttaccc agcgccatcg gcgtcaggta gtcggtggaa
     2881 attgtccaca tgccaaccca ggcgtgaacg ctgagcgcca ccagggtcaa caggctgaag
     2941 atacgcattg cgttattgga gaacagcgcc tgccattcgg cgtaacccag acccgggtta
     3001 cacaggataa agccaagcag aaacagcaca taaagcgcga ggacgacagc agatacacgc
     3061 tgcgccatcc agtcataaag acccgaacgc gagaaattcg tgacgttggt taccatatcc
     3121 acacccccag cagcacgatc acgatcgtcg acacgacgag aacgattttc gaacccagct
     3181 tgccgccttc cagcgactca ccgatgccca tgtccatgat caagtggcgt acaccggcca
     3241 ccaggtggta cagcagagcg gacaacagac cccaaatcac aaacttggcc agcgggctgg
     3301 tcaaacattc ctgcacctgg gcaaagccct ctgcggacga cagcgacttg tcgaggccat
     3361 acaacagaat ggcgataccg acaaagagga tgacaccgga gatacggtga agaatggacg
     3421 tgtaagcagt gattgggagt ttgatagtcc taaggtctag gtttacaggt ctttggctat
     3481 tcacggcttt tttatcacac tgagagcccc taactatcag ggcaaagttg tcgggaagtg
     3541 cactggtcag gtacccatca cccaaggagt gacaaccgcc aaaagacagg ctaaagcccc
     3601 tggcagctgg gcgcagagta tagacagtta ggttactaat gacaatgcaa agccccctac
     3661 ctaaaagcgc attgcgcgcc tctaataaat ggcgtaaaga gccgctttaa aagcccggaa
     3721 accacccgca aacccttctg ccgcctgggt tttcgcaaat tgacattcga atttatctca
     3781 ctatagtggt gcgggccctg cgtggggggc agtcagatga tttcaagcat aactaggagg
     3841 ccaaacatgg ctgacaaaaa agcgcagttg atcatcgagg gcgcagcccc cgtcgagctg
     3901 cccattttaa ccggcaccgt tggtcccgat gtaattgacg tgcggggctt aaccgccacg
     3961 ggccgtttca catttgatcc tggctttatg tccaccgcct cgtgcgagtc gaagatcacc
     4021 tatatcgacg gtgaccaggg catcctgctg catcgcggct accccatcga acagctggca
     4081 gagcaatccg attacctgga aacctgctac ctcctgctca acggcgaact gccgaacgca
     4141 gaagagaaag ccaagttcgt cagcaccatc aagaaccaca ccatggttca tgagcaactg
     4201 aagaccttct tcaacggctt ccgccgtgac gctcacccga tggccatcat gtgcggcgta
     4261 gtcggtgccc tctcggcctt ctaccacgac tccctggaca ttaataaccc gcatcaccgc
     4321 gaagtatcgg ccatgcgcct gatcgccaag atgccaacca tcgccgccat gacctacaag
     4381 tactccatgg gtcagccgat gatgtacccg cgtaacgacc tgaactacgc agaaaacttc
     4441 ctgcacatga tgttcaacac cccgtgcgag atcaaaccga tcagcccggt gctggccaag
     4501 gccatggata agatctttat cctgcacgcc gaccatgagc agaacgcctc tacttctacc
     4561 gtacgtctgg ctggctcctc gggtgccaac ccattcgcct gtatcgctgc cggtatcgcc
     4621 gccctctggg gcccggcgca tggcggtgcc aacgaggctg tactgagcat gctggatgag
     4681 attggcgatg tgtcgaacat cgacaaattc atcgccaagg ccaaggacaa gaacgatccg
     4741 ttcaaactca tgggcttcgg tcatcgcgtt tacaaaaacc gcgacccgcg cgccaccgtg
     4801 atgaagcaga cctgcgacga agtactgaaa gagctgggca tcaagaacga tccgcagctg
     4861 gaactggcga tgcgtctgga agaaatcgcc ctgaccgacc cgtacttcaa agagcgcaac
     4921 ctgtacccga acgtcgactt ctactcgggc atcatcctca aggctatcgg tattccgacc
     4981 agcatgttca ccgtgatctt cgccctggcg cggaccgtcg gctggatctc gcactggaaa
     5041 gaaatgctct ccagcccgta taagattggc cgtccgcgcc agctgtacac cggctacccg
     5101 cagcgcgaca tgaagaagta attctgtttc gccgcacaaa aaaggctgcc cgagggcagc
     5161 ctttttattg cgctgaataa gcccgcttta cggcttctca gccaactgac gcagcccctg
     5221 caaggtattg aacggtgcat ccacgacaaa gctgttagcc aaccaggccg gcacgctacc
     5281 gcccggttcg gaatgcactt ggtagatcac ctcgatttcg ccatcgcgcg gcgtaaggct
     5341 ccagaaaccc tcaaccttgc tcacccgcac aaagccttgc tgcagcggca aataatcggc
     5401 aaccccatgc agggtacggg ttatccgacc atcggcatcg cgcgtggttg tcacctgcag
     5461 gatcgagtcg cgcggctgta ccggccaggg ggtatgaaag cggctgtagg cccagcttag
     5521 atcgccctca tgcttgagca gcttctgctc gctgcaggca tgaatccagg cgcaggccgc
     5581 gctgacatca tcctgcaagg ccaataatcg cggcatatcc gctcgtatac gggtgacgcc
     5641 acgaaacgcc tgaaagcttg agcccgggat ctgctgtaaa taaacctgaa tacccgcctc
     5701 atccttgacc agacgccatt gcgccgcctg cgccccgctc accgccagca acgccgcaac
     5761 ccccaagagc agccctgtaa accgcaccat catgcctccg gcagcagata accttgcgcc
     5821 acagtatcag acttcaaccg ccgtgctctg ctccagccag ccaatcaact gcagggcatc
     5881 ggcggtattg ctaccgcaca catactcggc ggcatgaaac gccgaacaca ccgccggccg
     5941 ctcaggctgg ccgaagatca ggcagtagtt atttgcatcc agctgcacac aacgcacacc
     6001 cgccggcttg ccagcgggca tacccgggat gggggaactg atcgaagggg caatgcagca
     6061 ggcgccgcag ccggcacggc aattcatcga agggaaacct caaacgcagc acggtacaga
     6121 taagagcgcg aattttacgt tgcctggcca gacttagcag cccagaggat acagattcag
     6181 gcgagcggta ggcgcaggcg aaactgcgtg ccctgccccg gcgcgctgcg tacctgtaat
     6241 tcagcccaga gttaaccata gccaactgac gggtgaaggg acgcagtgca tgcctgaaga
     6301 gcgagagcag cgccgaataa tcgcagggtt cagcgcaata ggatggctgt tctcatcgcc
     6361 actcaaggct tatgcaggcg ggcaaacaga ctggaagtgt cccaacggcc gccgcccatg
     6421 ccctgcacat cggcgtaaaa ctgatcgacc agcgcggtta ccggcagctg cgcgccattg
     6481 ctgcgggcct cggccagcag aattgacagg tccttgcgca tccagtccac ggcgaaaccg
     6541 tgttcgaact cgccggccag catggttttg tgacgatttt ccagctgcca ggactgcgcc
     6601 gcgcctttgc tgatcacttc catcgcacca tgggcatcga gcccggcgca ctcggcgaaa
     6661 tgcagcgcct cggacaaacc ctgcaccagg ccggcaatgc agatctgatt gaccatcttg
     6721 gtcagctggc cgctgccggc ttcgcccatc agccgagcca tgcgcgcata agcctgaatg
     6781 atcggctcag ccttggcgta ggttgccgcc tcaccgccga ccatgatggt caacgcaccg
     6841 ttctgcgctc ccgcctggcc gccagatacc ggcgcatcga gaaagcccag ctggcgcgca
     6901 gcagcctgtt cggccagttc acgggctacc tgcgccgagg cagtggtgtg atcgaccagc
     6961 accgcgccgg gctgcatacc ggcaaaggcg ccctgctcgc ccagcaccac gccgcgcagg
     7021 tcatcatcat tgcccacgca gaccatcaca aactcagcac ccgtggccgc ttcacgcggg
     7081 gttggcgcgt ggctgccagc atattcggcg gcccactgcg cagccttggc ggcggtacgg
     7141 ttgtacacac acacctgatg cccggcgcgc gccagatgcc cggccatcgg gtaacccatc
     7201 acgcccagcc cgataaacgc cagctttgcc atatccacct ctaccttcat acgccatcta
     7261 caagcgcacg tttctgctta acctcatatc cccgctgcac ggcagatacg aacccggctg
     7321 caagcatgcc cgccacatgg ggccagcgcc agtacagcca tagggcaaaa ctgtatggat
     7381 acacccgacc aacaggcttt acccaatgcc gatccagctt ccatactcgc gacacgctaa
     7441 ggagggctta tgcagcattg gctggtaatc gacctggaag ctaccaccga agaaggcggt
     7501 tggccggtca cagatatgga aatcatcgaa atcggcgcca gcctggtcaa cgctggcggc
     7561 catgaaatcg agtatttcca acgctttgtg cgccccaccc ggcgcccaca cctgacgcac
     7621 ttctgccgtc agctcacgca gatcagccag gccgatgtcg atgccgcggc gccgctgagt
     7681 gtggtgtggg cgcaattcga acgctggttg ctggcccatc aagcgcaact ggcgggttgg
     7741 gccagctggg gcgactacga ccgtcgtcag ttcgagcagg aatggcacgc ccatggcctg
     7801 gagagttacc tgagccagat cccgcatatc aacctgaaaa agcgcttcgc cgaagcccgc
     7861 caactgccca gcccgattgg cctgaagatg gcgctgagtc tggccggcct gcagttccag
     7921 ggccagcagc accgcgctct gaccgatgca cgcaataccg ctcggctgct gccgctggtt
     7981 ttgcctgtgc aaagcagatg acgaagaaaa accgcttggg catactggcg ggccattttt
     8041 taaacccctt tcgaggaact gcagatgttc aaggtcaatg aatacttcga cggcaccgtc
     8101 aaatccatcg ccttcggcat ggttgaaggc ccggccacca ttggcgtgat ggctgcaggc
     8161 gagtacgagt tcggcaccaa ccagctggaa atcatgcacg tggtcgccgg cgccctgacc
     8221 gtaaaactgc caggcagcga aaactgggaa accttcacca gcggcagcaa gttcaccgtt
     8281 ccggccaaca gcaagttcca actcaaggtg gcggctgata ccgcttacct gtgtgaatat
     8341 cgctaagcca tagagcgcct gaaagccggc cttaatggcc ggctttttta ttaacggatt
     8401 aaacctcaag gaaccgccat gccgcgctcg acgcaattct ccctggtgat cctgccgatc
     8461 atattgctgc tgatcgccat gacctcgatc cagagcggcg cgtccctggc caaggggctg
     8521 ttcccggaga ttggtgcaag cggcacgact gccctgcgcc tgagcctcgg cgcgctgatc
     8581 ctgtgcattc tgatgcggcc ctggcaggcc aagctgacgc tgaagtccag ccgttccctg
     8641 ctggcctacg ggctgtcgct gggcggcatg aacctgctgt tctacctctc gctgaaaacc
     8701 attccgctgg gcatcgccgt agccctggaa ttcaccggcc cactcggcct ggcgctgttg
     8761 tcgtcacgcc gtctggtgga cttcgtctgg atcgccctgg cggttttcgg cctctggctg
     8821 ctgttgccca ccggacaaag cgatgtaccg ctggacccgg tcggcatggc cctggccttg
     8881 gccgccggtc tgtgctgggc gctgtatatc gtcttcgggc agaaggccgg ggctgagcac
     8941 ggcaagcaaa ccgtggccct gggcaccata gtcgcggccc tgctggtgtt tccgattggc
     9001 ctgtggcaag ccggtagcgg cctgttttca gtggacttgc tgcccatcgc tctggccgtg
     9061 gccgtgctgt cctcggccct gccctatagc ctggaaatgg tcgccctgac ccgcctgccg
     9121 gcgcgcactt tcagcatcct gatgagcatg gagccggcga ttgccgccct ctccggcctg
     9181 ctgttcctca gcgaacgcct gacgctcagc caatggctgg cgatcagcgc gatcatccta
     9241 gcctcggcgg gcgcggcggc aacgatcaaa ccgaaagcca agctgcttta aattcacgcg
     9301 cgcgcagctc tggtgggagg ggctttagcc gcgatcaggg tacagcggtt ttgaaccgtc
     9361 gcggctaaag cccctcccac aatgccaggc ccaagtgtta accaaccacc tcagcctcat
     9421 tgctaaattg caactcagcc aggcgtgcat acagcggact gctgctgacc agctcgctat
     9481 gggtgccgat agccgccagc ttgccgtgtt cgatcacggc aatgcgatca gcgcttttta
     9541 ccgtggccag gcgatgggcg atcaccaggg tggtgcgccc ctccatcagc ggcggcaagg
     9601 cttgctggat cagatgctca ctctcggcgt ccagagcgct ggtggcttca tcgagcagca
     9661 agatcggcgc atctaccagc aatgcgcggg caatagccag gcgttgacgc tgcccgccgg
     9721 acaaccccaa accggcatcg ccaagatgag tctggtagcc atccggcagc ttcatgatga
     9781 attcgtgggc atgggcggct ttggctgctg cctcgacctc ggccaggctg gctgcggttt
     9841 tgccgtagcg gatattgtct tccaccgagc caaagaacag cgcagggttc tgcgatacca
     9901 gggcaaagct actgcgcagt tcacgaggat caagctgctg gatcggtacg ccgtcgatca
     9961 ggatgcggcc ctgctgcggg tcgaaaaaac gcagcagcag atcgaacagc gtggatttgc
    10021 ctgcacctga tggcccaacc agcgccaggg tttcccctgc cgcgacctgc agatcgatac
    10081 cgtccacggc aaagcagccg gggcgcgatg ggtaggcgaa gcgcagttct tgaagttcaa
    10141 tacggccctg caccggctgg gtcagatgca aagcatccgt agccggcggg gtaatttcat
    10201 tgcgggcctg cagcagttcg gcaatacgtt cggcggcacc tgcggcgcgt tgcagctcgc
    10261 cgatcacctc actcaaggtg ccaaaggcag agccgacaat caggctgtag aacacaaagg
    10321 cggccagctc tccgccggaa atccgcccgg cgatcacgtc catgccaccg acccagagca
    10381 tcaccccgac tgcgccaagc accagcacga tcaccacggt aatcagccag gcgcgctggg
    10441 cgatgcgttg gcgcgcggtg tcgaaagccg cctcggcgga gaggccaaac cgacgcttgt
    10501 cctcattctg gtggttgtag gcttgtacgg ttttgatctg ccccagcacc tcaccgacat
    10561 agctaccgac atccgccacc cggtcctggc tctgccgcga tagcgcgcgt acgcggcgtc
    10621 cgaatatcag aatcggcgca accaccagcg gcagcgccat cagcacgatg ccgctgagct
    10681 tggcgttggt gactaccagc agcacgctgc cgccgaccag catgatcagg ttacgcagcg
    10741 ccatcgacag cgacgagccg atcaccgatt gcagcagtgt ggtatcggca gtcagacgcg
    10801 actgaatctc cgagctgcgg ttgctctcat aaaagcccgg atgcagttcg atcaggtggt
    10861 tgaacacgcg cttgcggata tcggcgacaa aacgctcgcc gatccacgac accaagtaga
    10921 accgggtaaa ggtgccgatg gccagcgcca gtaccagtac gaaaaacaac ccgatggatt
    10981 gctgcaacgc cgccggcgac tgggtcgcca gaccctggtc gaccaacagc ttgatgccct
    11041 ggcccatcga caaggtgatg gcggcggtaa acatcagcgc cagcaaggcc ccaaacacgc
    11101 gaccgcgata cggcgcgata aagcgccaag ccatacgcag ggcattgcgc tgacgggaag
    11161 acaggatcga aagcatgaag gctcgctggg gcgctgacgc ccgttaatga ggaggcgatt
    11221 gcggcgtttc cgcccgccag accacccaac acagcgccaa agcggcgaga ttggtggtca
    11281 gcggattaaa cgcctgcagc agtagatggg gactgaatag cgccacatca agcagcagcc
    11341 caatcaacag cacaccggcc agcaacaaag gccagcgctg acgtcgccaa agcagcaaaa
    11401 gacccaggat gatttccgcc agaccggcga tgcgcgccac ccactcgggg gctggcagat
    11461 tgtgggcctg aatcatcgcc agctcatccg ggcttaacca gaggatcttc ggcaccaggc
    11521 cgtgccagat aaacaccaga gccagggcga tacgcgctac ccaggcgatc tgccgtaaac
    11581 ggggatcagt catgcaaaaa gcgccgggca tgatcggcgc ttggcaacag gcaactatcg
    11641 tagcgaccaa gtagccggta gcgatttagc gcaatacggt catagcacca gtcacgtatg
    11701 ggccgtggaa tcagccgcag ccaggccagc aagcgccatg gcttaggcag ttgggcgacg
    11761 atgcgcaaca ccgcgtccga acgcagcagc atctggccat tctcgatcag cgccatactg
    11821 tcgaaatgat cggtcggcaa accgccccag accaatagcg cctggccctc ggccgactgc
    11881 accgaggcca ggcgaaagcg ctgctcaacg tcatgacgaa tcagaaactt agcccagcca
    11941 ttgcacagct tacacacccc atcaaacagc accacgcgct cgccgggctg aataaggggt
    12001 ggaagtgttt gctcctgcat gcccaactcc ttctgcccgg cctctaggcc gcagggatac
    12061 ggtaatggcc ggtaatttta aagctgaaga gaatgtcctc ttcctgggta ccacggccaa
    12121 tattgtgcag caccaggggc acgccgctgc cggcctggcg gtcgctgata atgccgatat
    12181 gggtcaggcc gccgcccagg tcccaggtga cgatatcacc aggccgatag cgctcggcat
    12241 cctgaccaag cggcagcgac cagccctggc gtttgaacca ggtcatcaga tttggcacgc
    12301 ggcgatggtc gatattgctg tcgggccggc ttagccccca gttcttggga tacaggcgaa
    12361 agttcgcgcg catgtcctga tgcaccgcct gctgcaagtc gagtccctgc tggcgcagcg
    12421 cgcggattac tacatctgta cacacgccgg tagtcagcgg cacatcgcca ttggggtagt
    12481 tcaggcgtcg gtaggcgggg tcgtaactca gggtcacgcc aacctgctgg cgggcagccg
    12541 tgaccagggc atcggcctgc agcgcccagg cctgggcgct tagcagaata ctcaatgcaa
    12601 ccagcagaat cgcccgcata acgcctcctt aatagttcag ctgctacttg cctacgggat
    12661 agagcagctc ttccttataa ccggcccata cgcgcaccac atcagggaaa gcatcgtcca
    12721 gcgcgacatc accgctgtcc accagcaacg gccggccctc cagggtgccc agtttgcgct
    12781 tggtggcgac cacacgcagg ttttccaggc cgatggcgcg cagcacacgt ggactgatct
    12841 gctggttgcc gcggccgatg atatgaccct ggccgccgat ggcagtgacc agcagacgcg
    12901 ccggatggcc atcaaccaac tcaaatagct gcgtttcgct tacatcgctg gccagcagtt
    12961 ggccgttctc gattacatcg acgccgagca aggtggtctg cacgccgagg ttctgcgcca
    13021 ggccatgcag ggtcgaaccg gggccgaaca catagcgcac atcggtttcc cagctgtcct
    13081 gcagccagtc ggccagatca cccaagacca gctcttcgga ctccacgccg ccctgcttga
    13141 cctgctgcac atagccgcct tcctgcggca ccgtcagctc gccgtaccag cgcgcagtca
    13201 cacggccttc gcgcagggcg ctttcatcaa tatcgcgtac ctcgccactg ctcaggcgca
    13261 ccaggccgcc ttcgatcagc cgcgcagtca attcgccggc agcgcgcggg ctgatggcat
    13321 agaccccgga ctgaatcttt accccagccg gaatccccaa caccggctgg ccttcgcgca
    13381 ctgccgcgca gatatcccgc gccgtaccat cgccaccggc gaacagaatc agcgccacgc
    13441 cggcatcctg cagcagctca accgcctgac gggtatcggc ggcgctactc gggcctgcgc
    13501 ccaattcacc aaccacacgg tgggcaaagc ccatctcgct cagcagatcg gcgcccatgg
    13561 cgccgggaaa ggtgacgaat tccaggcgcc cacgcagcgg caacaacact tcaagggcgg
    13621 tttgcgtacg cagcgccgac ttggcctcaa cgcccagagc caaggcctgt tcggccatgc
    13681 cgtcactgcc cttgaatccc gcggggccgc ccaatccggc caatgggttg atgatcaagc
    13741 caatatggaa aagccccata acaccctcta attcattaac tgactgcgcc tggcacagtg
    13801 cctgcaacga ctgacttgcc ctgtgtcttg cggcagtttt ttgccgtcgc agttgtcata
    13861 gcgccttcat ctaacactca taaaatcgcg ctaaccgatg aggagacaag ccatgcacag
    13921 ccaacaacag aactcgcaaa aagccacgac aatgccgatt ggtggctttg tcatcgacgc
    13981 ccagggccgt gaaattccga tcaccgaaga gatgatccaa caggcctgca gcgcgctgga
    14041 agaagcccag cagcaagccg gcaagcgtaa gtaagtttcc cgccagggca gcacctgctg
    14101 cccttcgcta cagctaattc aacttcgcac ctaacgcctg caccaggctg gccaacgccg
    14161 gcgcatcacc cttcacgcaa acctccaacc cgtcgatctc ccggcgcatc gggtagtgtt
    14221 tgcgcagccc gtcaaaagcc gcacgccggg ttgcggtatc gccctgcagg ctacggcgaa
    14281 agtccgcgtc atcgcggcgc gggtcatata cggcgcggca gatagtcgcc agggcccagg
    14341 ccggatcagc gctggcatcg acagccagct cactcagcca ggccgccggc atcagttgcg
    14401 ccaactcaat gctgggcgca acgcctaggt gctgacacag cgcttgatag atctgcgccg
    14461 tgccgcgcag cttgccgtcc aggctgtagc cggcgatatg cggcgtggcg atgcggcaca
    14521 acccagccag ctcgacatcc gcctgcggct cgccttccca gacatccaat accacctgca
    14581 aatcactgcg ctgagtcagt aattcacgca aggcctggtt atccaccact gcgccccggc
    14641 tggcgttgat cagccagctg cccggcttca gcgcggccag gcgcgcggca tcgaataggt
    14701 gataagtagg atgctcgccc agacgctcca gcggcgtatg caggctgatc acgtcgcact
    14761 catccagcac ctgctgcagg cttacataat caccgccctc agctgcctgg cgcggcggat
    14821 cgcagaccag aacacgccag cccaggccac gcagcacctt aagcagacga ctgccaacct
    14881 gccccgcgcc gataacgcca taagtacggc tagccagatc aacgccctgc tgctcggcca
    14941 ataccagcag gctgcccagc acatagtcga ccacgccacg ggcattgcag cccggcgcgc
    15001 tggcccaggt gatgccagcc tgctggaagt aattgaggtc cagatgatcg gtaccaatgg
    15061 tacaagtgcc gacaaacttc actggcgagc cgcccagcag cgtgcgatta acctgagtca
    15121 ccgaacgcac caacagcagg tcagcatcgg cgaccgcagc cgcatcgata ctgcggccgg
    15181 gcaggcgacg aatctcgcca aaaccggcaa agaattcatc aagcagggga atgttttcgt
    15241 cggcgactat ctgcatgggt ttgctccgca aatcaggccg gcattctaac ctgcctgcac
    15301 cgcttggcta cgctgagcaa cgcaatgcgt ccttcgcact gaaacactgt gcgacaaggt
    15361 ttcctgacca agccgtcagg gcgtagactg cgcggtttcc cgacataccg ccggatacat
    15421 atcaatgctt gttccaaccc gcttgcaccg cgccagcacc gagctctacg gcctgctgaa
    15481 actcgcaacg ccgatcatca ttgcccagtt ggccaacacc gccatgggct tcgtcgatac
    15541 cgtgatggcc ggccgggtca gcccacagga tctggctgcc gtggcgctgg gcaactcgat
    15601 ctgggtgccg gtttttctgc tgatgaccgg aattctgctg gccaccacgc ccaaggtcgc
    15661 ccagcgcttc ggcgcaggcg cgcataccga gatcggccca ctggtgcgcc aggcgctgtg
    15721 gttggccctg gccgtgggta tcgcagctgc agtgattctg tggaatgcgg aaatcgtcct
    15781 cagtctgatg aacgtcgacc cggcgctgat cgaacccgcc atgggttacc tgcgcgccgt
    15841 ggcctgcggt tttcccgccg tggcgctgta ccacgtgctg cgttgcttta gtgatggact
    15901 cggccacacc cggccgagca tggtcatcgg cctgtgcggc ctggcgctga atattccgct
    15961 caattacatc tttatctacg gcaagctcgg cctgccggcc atgggcggcg tcggctgcgg
    16021 ttgggccacc ggcctggtaa tgggctttat gtttttgagc atgctctggt gggtgaagtg
    16081 ggcgccgttc tatcagccga gcaaaatctt cagccatttc gaatggccgc aatgggccgt
    16141 gcacaaacgc ctgctctcgg tcggcctgcc gatcggcatc tcggtattcg ccgaatcgag
    16201 catcttcgcg gtgattgccc tgctgatcgg cggcctgggc gcgactgtag tggccgggca
    16261 ccagatcgcc ctgaacttta gctccatggt gtttatgatt ccctactccc tgggcatggc
    16321 cgccaccgtg cgcgtcggcc aggcgctggg ccgtggcgag ccgcgtgaag cgcgctttgc
    16381 ggctggggtg agcatggcgg cggcgctggg ctacgcctgc ctgtcggcca gcctgatgct
    16441 gctgttgcgc gaagagattg cgcagatcta taccccggac cgcgcagtaa tcgccgtggc
    16501 caccaccctg ctggtgtatt cagcgctgtt ccagttctcc gatgccattc aggtcaccgc
    16561 cgccggcgca ctgcgtggct atcaagacac ccgcgtaacc atgatcctga ccctgttcgc
    16621 ctattggggc atcggcttgc cggtgggata cgcgctcggt ctgaccgact ggttcggcga
    16681 gcccagcggc cccagcgggc tctggcaggg tcttgttgtc ggcctaacct gcgccggcgg
    16741 catgctcgcc gtgcgcctgg ccggcagcgc gcgcaaacgc attcgagccg ctaagatggc
    16801 ctgatgaatg tccgcctgct gcttctactc gctgcacttt gcctgtgcgc ctgcacgccc
    16861 gccgatgatg gcttggcgct gcaggccgat tacctgcagc gcttgagcaa tgccctggat
    16921 gtcgaacccg caaccgcctt cgactccagc gagctgactc gctatcgcct gccagcccgc
    16981 cgtgaacgct tgatcgagat tgccgaattg cgcatcagct tgctggacct gctggtcgat
    17041 gtgcgtcgtt gccctgccct gcaacagcag atcagcctgc gcaacaacag cctgggcaaa
    17101 cagctgacgc ccagcagccg cctggcctat gaaggcgatc tgctgcgcgc cctggatgcc
    17161 tgcagcgcac acctgcgcag ccaggatcag caaccactgc gcaacaccct tgaacaactg
    17221 gcgcaggaaa aacgtgcgca actgcccggc gtattctgga atgccctgaa tgccagcccg
    17281 gaggtcgaac gctacctgcg ctttgccgaa cagcccctgg caccaaatgc cgacgaagat
    17341 aacgcggcgc tggatgccct ggcgcagctg gccgccatcg gcggcgcgct acctgatgcg
    17401 ttaccgccga gtgccgaaca gcttgatcca ttgttcttcg ccctgcatgc cagcgaccag
    17461 ggcggtcagt tgatcagcag cctggccagc ctgacccata gcctcaacca gggcagcgag
    17521 atgctaaaca gccgccaaca gcgccggccg ctttgcccca cgggcaaggc cacagaacgc
    17581 gcgcgcatcg tgcagaacat cttcgtcaag tattacgcgg gcagcctgca gccctatctg
    17641 gcgcaggtgg accagcgtgg ccaggtctgg agcagcagcc tgcgtagcct tagcagggtg
    17701 atgcagatac cgccggccac tcagcagtac ttgcagagcc tgagcggccg agaggcttcg
    17761 ttatgggcgg atttccagca ggccaccgcg cggcatgtgc acagctggca ggagctgctt
    17821 aatagctgcg gtctggctcc cggtcagtcg ggatggaaag gcaataccgc gagcgccgac
    17881 cagtaggcct cggacaacaa cgcctgcccc gccagtagat gggccaggta atcggcgctg
    17941 ggtagcagac cctctgcgcg aaaggccggg ttagcgatat acacccaggc cggctgcacg
    18001 ccatgggccg cgacaatcgt caggcgctca cggctgtaat agatcggcgt gccttcgaag
    18061 tgatccagct tggcgatctc gccagcgtcc tgcaggcgat agagcacgcc ttccacccgc
    18121 ccatcacgct gatgccgaat gttggcgtag gcccgacccg gcgcccgatc cgccgcccgc
    18181 ttgttaaagc acaggctgta acccggcaaa tgtccaggca gtgcctccag taccgtcagg
    18241 ccgcgagcct gcatgcgtgc caagctcata ttcgagccat aggcgaagta ccaattcatc
    18301 agtcgagctt cttgcgaatc agatagagat aggtcgcgcc gtcttccgcc tgctccacca
    18361 gctcatggcc aagaaacacg cagaatttcg ggatatcgcg gcgggtcgag gggtcggtgg
    18421 caatcacctt gagcaggccg ccggcctgca ggtcgcgcac cttgttgtgt agcatcatca
    18481 ccggctcggg acagttcagc ccggtggcgt cgagggtggc atcgggaatg gcggtggttt
    18541 gcaaagacat cggcaggctc cagtaacagg ccgctattct ggcgcaaagc tgagccaaag
    18601 gtcatcccgc aaagcgacac aaccttggat gggcagccgc aacgggtcac ttgaggcagg
    18661 ctgcgcagcg ccaactattc caataagagc catttatgcg ccttgtcctg ctcctcgcca
    18721 ccctgctgct cagcggctgc gccgcccgtg accaactgcc ggatttcagc gccagcgcac
    18781 agcaggacct gcctgccaga tacttcgatg ccgttatcta tggtcgcgac ggcacacgcc
    18841 tatctgccac ggtgttccag cctgcgctca aggtcggcga acaggcgccg gtgattgtgc
    18901 atacccatgg ctggggcggc tggcgcgtaa cccggccgaa cggtttctac ggcacccaga
    18961 tgatgtccgg ccgcgccgca gtgaaagcct ggcaagccgg ttactgggtg atcagctacg
    19021 accagcgcgg ctggggcggc agcgagggcg acatcgaaat gatgaacccc gagtacgaag
    19081 tgcaggacgc cctggcggta atcgactggg ccgccgccaa cctgccacgc ctgagcatgg
    19141 acgcgccgaa tgaccccaag gtcggcatgc tcggcgaaag ctacggcggc gcggtacagc
    19201 tattggcctc ggcgactgac ccgcgcatcg acgccattgt gccgattgcc acctggtatg
    19261 acctgagcga agccctggcc ccggacagcc atatgaaggt cggctggacc agcgtgctgc
    19321 tcagcatggg cctggcgacc ggttatgacc tgggcaagtt tatccagccc ccctacctga
    19381 aaagtgtcga tggccgcatg cgcgagccgg tgcgcgccga gctcaaggcc cacagcctgg
    19441 ccagctactg cgcgcgcggt caacgcccgc aagccgatgc cctgctgatt cagggcatgc
    19501 gcgacaccct gttcccgctc aatcagggcc tggcgattcg cgactgcctg cagcagggcc
    19561 cgcacgatgt gcgcctgctg ggtatgcagg gcgggcatat cctgccgccg cccatgcaac
    19621 gctggagcgg cctgccgccg ttcaacaacg aagcggtgct gcactgcgat ggccgggcga
    19681 tcaacctcta ccagagcatc atcagttggt atgaagacaa gctgcgcggc cgcaagggcg
    19741 tggcggcgag cattcccgat ttctgcgtca gtctcgatct ggatcagggc gtggccttgc
    19801 agcaactgcc gggcaatcag cggatcgccc tgcccgccac ctctattcgc ccgcttagca
    19861 gcgggctgct acaaccgtcg cgctttatcc cactgcaaca ggtacagacg agcagcgccc
    19921 tgatcggcgt accccgtgtg catctggata acgcggcaca attgccaacg ctgtttgccg
    19981 ccctagtggt tcggcgcgcg gatggcagta gtgaggtgct gagtgagcag gtcaccccgc
    20041 tcaagggcca ggccaatacc gaattagctg ccgtcagcgc gcaactgcag cctggtgatg
    20101 tgctgggcct gcgcatcagc ggctttagcg gccagtacct gttcaacagt tcctggcagc
    20161 tctccagcag caccctgcaa gggacgatcg aggtgccgca gttgctgccg ttacaaccgc
    20221 aactggcccg gcattgagat cacttgccta accggcgcag gtggcaggtc acttcttcac
    20281 ggtcgtgata aagctgtttg caggcaatcg agaccttgat cttgcgctcg gcaaaaccat
    20341 cttccagacg ctgtaacagg cggcgcacct cggcgtagcg ctgcttcatc ggcaacttga
    20401 ggttgaccac cgcctcacgg caatggcctt cgccgatcca ggtctccagc aaggcggcgc
    20461 tgcgggcggg tttttcgacg atatcgcaga ccatccagtc caccggctgc ttgggcttcc
    20521 aagtaaagcc gtcaaccatt agatgggtca ccaggccggt atccatcaag ctctcggcca
    20581 tcggcccgtt atcgatagcg gtgaccagca tgccgcgctt gactagctgg taggtccagc
    20641 cgccgggtgc agcgccgagg tcgaccccgg tcatctcgtc cgacaggcgc tcatcccatt
    20701 gctcgcgcgg gataaatgtg tgccaggcct cttccagctt gagggtcgag cggcttggcg
    20761 cttcgcgggg gaacttcagc cgtggaatgc ccatgggcca gatcgccgag ttgttcgcct
    20821 cggccatgcc gacaaacacc tcgcggccac ttttgaaggt cagcagtagg cgcggcttgt
    20881 gcgcatcctc gaccagcttg ccggccttgg tcagggcatt gcgcagcggc gcctcgaact
    20941 tcttgcagaa attcgacagc tccttgccat cgttggtgtc cagcacttcc agccacaggc
    21001 tgccgaatac cggaaaatcc gccagatggc cgagcagcac gctgatgcgg tccagctcgg
    21061 gcaggttgat aaacaccccg cgcgcccact gccgggggaa gatcagctga ttgaagcgca
    21121 ccccgcgcat caggcgctcg gcgccatcgt cctcggtgca gataaattcg gcgcaggcgg
    21181 tgctgggttt ggccttggcg taaccggcaa cgtccaaacg cgccgcatgc tcggcgatct
    21241 ccgcacacac ctcgttttcg aaaccgggac ggcagtgcag tagcaaggta ttcatagggg
    21301 ctctcctcaa ggcgcgcatc atagccgagt cgggaaccct cagtgcctgc aacctgtcca
    21361 gttaaagggc agcactcgcc aaacagagct agtttaagac tctgttgcag acaatccggc
    21421 ttaccccgtg acaagcccta ggagacgcgc aatgccttcc ttaaatagcc tgaacagccg
    21481 ccgcagcctc gatatagacg gccagaccta ccactacttc agcctccccg aagccgccag
    21541 agccctcggt aatcttgacc gcttgccgat gtcgttgaaa gtcctgctgg aaaacctgct
    21601 gcgctgggaa gacggcaaga ccgttaccgg cagcgacctg caagccatgg ccgactggct
    21661 gaagaaccgc agttcggaac gcgagatcca gtaccgcccg gcccgcgtgt tgatgcagga
    21721 ctttaccggc gtgccggcgg tggtcgacct ggcggccatg cgcgacgcca tggccaaggc
    21781 aggcggcgac ccgcagaaga tcaacccgct gtcgccggtc gatctggtga tcgaccattc
    21841 ggtgatggtg gataaattcg ccagcgccgc cgccttcggc gagaacgtcg ccatcgagat
    21901 gcaacgtaac ggcgaacgct atgcctttct gcgctggggt cagcacgcct ttgacaactt
    21961 cagcgtggtg ccgccgggca ccggcatctg ccatcaggtc aatctggaat accttggccg
    22021 caccgtgtgg acccaggaag aagacggcca gatgttcgcc ttccccgaca ccctggtcgg
    22081 caccgactcg cacaccacca tgatcaacgg cctcggcata ctcggttggg gcgtcggtgg
    22141 catcgaggcg gaagcggcca tgctcggtca gccggtgtcg atgctgattc ccgaggtgat
    22201 cggcttcaag ctgaccggca ggctccgcga aggcattacc gccaccgatc tggtgctgac
    22261 cgtcacgcag atgctgcgca gcaaaggggt ggtgggcaag tttgtcgagt tttacggcga
    22321 cggcctggcc gagctgcccc tggcggaccg tgcgaccatc gccaatatgg ccccggaata
    22381 cggcgccacc tgcggctttt tcccggtcga tgacatcacc ctgagctacc tgcgcctgtc
    22441 cggccgcccc gagcaaacgg tgaaactggt tgaggcctac tgcaaggctc agggtttgtg
    22501 gcgcgagcaa ggtcaggaac cgctgttcag cgacagcctg agcctgaata tggatagcgt
    22561 cgaagcctgc ctggccgggc caaaacgccc gcaagacaag gttcccctac gcctagtcaa
    22621 acagactttt gacgacttcg tcggcacacg gctcaaacca ccggccaagg aagaaggtcg
    22681 catgcttagc gagggcggtg gcggcaccgc cattggcagc gacgaatcac ttagcgagtt
    22741 cgaatatgag cacgagggca cggttcatcg gctgagcaac ggcgccgtgg tgattgccgc
    22801 catcacctcc tgcaccaaca cctccaaccc cagcgtgatg atggctgccg gcctgctggc
    22861 gaaaaaggcc tgcgagaaag gcctgcagcg taaaccctgg gtcaagagct ccctggcgcc
    22921 tggttccaag gtggtaaccg attacttcaa ggctgccgac ctgacccgct atctggatga
    22981 gctgggcttc gatctggtcg gttatggctg caccacctgc atcggcaact ccggaccgct
    23041 gctcatgccc attgagcagg cgatccagca gcacgacctg acggtagcct cggtgctctc
    23101 cggcaaccgc aacttcgaag gccgcgtgca tccactggtg aaaaccaact ggctggcctc
    23161 gccaccgctg gtggtggctt acgccctggc gggtagcgtg cgtctggacc tcagcagtga
    23221 accattgggc atcggcaaag acggcgagcc ggtgtacctg cgcgatatct ggccaagcca
    23281 ggcggaaatc gccgaggccg tgcagcgggt cgataccgcc atgttccata agcaatatgc
    23341 cgaggtattc gccggtgatg cgcaatggca ggccatcgag gtgccgcaag ccgacaccta
    23401 cgcttggcag gccgactcga cctatatcca gcatccgccg ttcttcgaat ccatcggcga
    23461 ggcgccgccg catatcggcg atatccgtca ggcacgcatc ctcgccctgc tcggcgattc
    23521 ggtgaccacc gaccacatct ccccggccgg caatatcaag gcggacagcc ccgccggccg
    23581 ttacctccgc gagcaaggag tggcgcctgc ggacttcaac tcctacggtt cgcggcgcgg
    23641 taaccatcac gtgatgatgc gcggcacctt cgccaatatc cgcatccgca atgaaatgct
    23701 cggtggcgag gaaggcggca acaccctgca tgtgccgagc ggcgagaagc tggcgatcta
    23761 cgacgccgcg atgcgctatc aggagcaagg cacgccgctg ctggtgattg ccggcaagga
    23821 atacggcacc ggatcgagcc gcgactgggc agccaagggc accaatctgc tcggcatcaa
    23881 ggcggtgctc gccgagagtt tcgagcgtat ccaccgctcc aacctggtcg gcatgggcgt
    23941 gctgccgttg cagttcaagc ctgggcaaac ccgcaaaacc ttgcagctca gcggcaggga
    24001 aaccctcaat atctgcggcc tcgatggcgt cgaactgcat ccctcgatgc agctaacgct
    24061 ggacattctg cgtgaggacg gcagtcggga cagcattgac gtgctgtgcc gcatcgacac
    24121 actaaacgag gtcgagtact tcaaagccgg tggcattttg cactatgttt tgcgccagct
    24181 gatcgccggt tgaagcccgg caaacgcggg tgcgcaaagg ccgcgcccgc tgcgcgctta
    24241 cgccgcaaaa ggtgaggtcg ttcaatatcc agttacactg cgaaccatat tttttgctca
    24301 acttcgggcg cgcccggccg atgacaggca taacttttag ggataaaaca cccatgcgca
    24361 ataatcaacc ggttacccag cgcgagcgca ccttccccgc gcagcaacga ctgatctcca
    24421 ccaccgacgt aaaaggccag atcacctact gcaacgacgc ctttgtcgag atcagcggtt
    24481 ttagccgcga tgagctgatt cgtgcgccgc ataacctggt gcgccacccg gacgtaccgg
    24541 ccgcggtgtt ccaacatatg tgggacaccc tgaaaaaggg ccggccgtgg atgggcatcg
    24601 tcaagaatcg ttgcaagacc ggcgaccact actgggtcaa cgcatacgtc accccggtga
    24661 cagaaaacaa ccaggtggtg ggctacgaat cggtacgggt caaaccgact gccgagcaga
    24721 tacgccgcgc cgaggcgctg taccaacgca tcaacagcgg caaatcggcg atccctagca
    24781 gtgatcgctg gctgccaatc gtgcaggatt ggctgccgtt tatcctggtc agccaggttg
    24841 gttttttgat cggtgtgtgg ctcgactcca gctggggctt tgccatcgcc gcaggattgt
    24901 cggtaccact gggcctggcc gggctgaact ggcaacaacg cggcctcaag cgcctgctgc
    24961 gcctagccga ccagaccacc tccgaccctc tgattgcaca gatgtacacc gacagccgcg
    25021 gcgctcaagc ccgcctggaa atgtccatcc tcagccagga agcccgtctg aaaacctgcc
    25081 tgactcgtct gcaggacacc gccgaacagc tcacccaaca agccaaacag gccgataccc
    25141 tggcgcacaa cagctctgcc gggcttgagc gtcagcgcag cgagaccgac caggtggcca
    25201 ctgcgattaa cgaaatggcc gccactaccc tggaagtggc gagtaacgtc gcccgtactg
    25261 ccatcgctac ccaggatgct aaccgcctga ccagcgccgg ccgcagcatt gccagcgaaa
    25321 cccgccaggc cattcagcgc ctgtcgcaat cggtcggtga taccggtgaa acggtgaccc
    25381 gcctggccca agacagcaat gaaatcggcg gtgtggtcga tgtgatcaaa ggcatcgccg
    25441 accagaccaa cttgctcgcc ctcaacgccg ccatcgaggc cgcacgcgcc ggtgaaatgg
    25501 gccgtggttt tgccgtggtc gccgatgaag tgcgttcgct ggcgcagcgc accgccgaat
    25561 ccaccgggca gatccaccag ctgatcgcca agctgcagcg caccgcagaa gaggctgtac
    25621 tgaccatgga aatcggccgc aaacaggccg acgaaggggt tgaacgcgtg cagcaagccg
    25681 atgaagcact ggccggcatc agtgacgcgg tagccaatat cgccgacatg gccaaccaga
    25741 tcgccgccgc cgccgaggaa cagagcgcag tggccgatga ggtcaaccgc agcatcacca
    25801 ccatcgccca actggccgac aagaccgctg gcgaagcgca tgacaccgcc ctgctaagcg
    25861 aagccctgac ggccacagca cacggtcagt acgctctggt ggaacgcttc aaccgttagc
    25921 ctgtaaccgc gcactgccct gcagtgcgcg gccctccccg ttgttaaaaa ttctcgtagc
    25981 gattcatatc cagcacaccg gcttccagcg gctcggtttg ctggatatac gccgacatat
    26041 cgtggaagta gcgccagaac tccgggtgag tgcggcgaat gccccagcgc tcgacgactt
    26101 tctcaaaagc cactgcatca cgggcctgct ccagcgccgt gacaaactcc gccgcctgcc
    26161 ccacctgcac attgaagata aagttcgggt agctgctgag cacccccgga tacaccgtca
    26221 gggtatccag cgcgggctga tagcgcagtt cttcaccgag cataaaagcc acattcgtgt
    26281 gtgctcggtt gcgtagcagg ctgtacacct cacgctgacc attagccatt tccacccgca
    26341 gcaaagtcgc ctccggcagt tgctcgatga ccttgagcgt gcccgcaggt ttggctgcca
    26401 gccggctcag ggactgctcg gcacgctgca gctcggcgtc cagccccgag cgatggcaat
    26461 gtgcgccggt gcagcggttg atcgggtccg gccgcgcatt caggctgccg tagcgttcca
    26521 gcagagtctc gacaaagctg cgtttggggt cgttcccagt caggcgcaag gcgctcgggc
    26581 tttcggtgtc tatggcccgg taatccagcc agatcttcaa cctgccgctg ctctggtacc
    26641 agtcatccag gtagccctgg cgcgactcgg ccggcagcaa acggaggaag ttgacctcgg
    26701 caccgttgcg gatcaaatcg aagtacagcc gcgtctgcgc ctggtgcgaa acgttgccaa
    26761 acacgtcgaa attcaccacc agttggtaat aggtgcgctc cagcaacggg aagtccagcc
    26821 accagagggt ttgcggcact tcaccgatca ggcctttgcg caccgaggcg ctgtcatgct
    26881 ggcggaagat cgacagcagc gcgttgtcat taccggccca gatatgcgac cagctcggcg
    26941 cgggcgcatc ggcatagcgt tgcatgcgta gcgcttcgta ctcattgcgc ttgtcgcggt
    27001 aggtgcgcca caggccgatc atgtcaccga tgctgtccag ttgcccgggc atggccagca
    27061 aatccttggc ctgctcgcgg tactcgggat cggtgatgta aaggtcgtgc cgcgggtcct
    27121 ggaacaccgc ccagaagtta tcgcgaatca catccgtggc gatctgccca cggcacaccg
    27181 ggccgcgaat aaaggtgcgc acgaagtatt cagcgttatc cagcataaat tggtagcgcg
    27241 cctgcggcgg aatcgcggca aaggtctcga acgggttggc gcggcgtagc aggctatagc
    27301 ccggcaccgc gtccgcccgc caatcgctgg cgaaaaacag ctctttaacc cgcgccagct
    27361 tcgccgcgct cagcccatag gtgatatggg tcttgtgcac gatcacaccc tgcaccggcg
    27421 tcaggcggta atagaactcg ttgccggggt cttcgttggg caggcgcgtg gcgatgatat
    27481 ccaccggctg accgctgggc gtgcgcgaac gcaccatctg gaagaaatgc cctggctcgc
    27541 cgctggcgaa atacagatgg gcaagaaaca gatgctcgta gagccagcgc gaaaccagcg
    27601 cttcacgggc gccgggggcg ttcataaagg cttcccaggt ggagatctgc tgcgcctcgg
    27661 ctgtgctggc ctgcagcgct tgctgatcga ccggcgcccc ctgctccagc caagtctgca
    27721 gcgtctggta gtcggcctca ctcaagccgg tcaccgcgaa cggcatgcca gcttgcggat
    27781 tgtcccgggc aaacttgtca aattcctccg gcagcggaca ttggttgacc cggtcgatgc
    27841 tgatattcag ctcagccggc aatttgctgt tggcagcaaa cggctgctga tggcccaact
    27901 cgagcatgcg cgccatcagc gctgcctgac taccctgtga gtccagcacg gaggtaaagc
    27961 ccttgcgctg ccaggcagca gggctatgag catcaacaaa caagcgcgtg gtggcctgcg
    28021 cctgggtgcg cgtgccgttg tacaccgtgg ccttattggc gccgcgctgc acgccttcgc
    28081 cactgcccag gttgagctgg caggcggcgt cgtaacaggc atggcaggcc acgcagttct
    28141 tggtaaaaat cggttgaata tcgcggctgt aggagatggg ttcagccgct tgcaatgagc
    28201 cgatgaacaa caaacacacg ccaagcagta tgcggcagac catggaaaca tctctaaaat
    28261 ggcttaagcg ccgattctag ctggcgccgg ctggccaagg ccaatcatgc gaacatgagc
    28321 cagattcatg taaaagcgca gaccactgca aaagcgtgca gctttgttat catcgcgcat
    28381 ctttttttca cgcgtcccag gtagttatct ccatgtctct gtccgatcgc agcgcccgcc
    28441 ttaacgcact tcagcaagcc ctgaaggaac gcatcctgat cctcgacggc ggcatgggca
    28501 ccatgatcca gagctacaag ctggaggaag aggactatcg cggcacgcgc ttcgccgact
    28561 ggccaagcga cgtcaagggc aacaacgacc tgctgatcct cagccgcccg gacgtgatcg
    28621 gcgccatcga gaaggcctac ctggatgccg gtgccgacat cctcgaaacc aacaccttca
    28681 acgccaccca ggtatcccag gccgactacg gcatgcaggc gctggcctat gagctgaacg
    28741 tcgaaggcgc gcgcctggcc cgtagcgtgg ccgatgccaa gaccctggaa accccggatc
    28801 gcccgcgctt cgttgccggc gtgctcggcc ctaccagccg cacctgctcg atctccccgg
    28861 acgtgaacaa ccccggctac cgcaacgtca ccttcgatga gctggtggaa aactacgtgg
    28921 aagccacccg cggcctgatc gaaggcggcg cggacatgat cctgatcgaa accatcttcg
    28981 acacgcttaa cgccaaggcg gcaatcttcg ccgtgcagca ggtgttcgag gacgacggtg
    29041 tcgaactgcc gatcatgatc tccggcacca tcaccgacgc ctccggccgc accctgtccg
    29101 ggcagaccac cgaagcgttc tggaactcga tcaaccacgc caagccgatc tccgtgggcc
    29161 tgaactgcgc cctcggtgcc aaggacctgc gcccgtatct ggaagagctg tcggccaagg
    29221 ccggcaccca tgtgtcggcc caccccaacg ctggcctgcc gaacgccttt ggtgaatacg
    29281 atgaaacccc ggcggaaatg gccgcagtgg tggaagagtt cgccgcctcg ggctttttga
    29341 acatcgtcgg cggttgctgc ggcaccacgc ctgcgcatat ccaggcgatt gccgaagcgg
    29401 tgagcaaata cacgccgcgc gccatcccgg atattcccaa ggcctgtcgc ctgtccggtc
    29461 ttgagccctt caccatcgac cgcaactcgc tgtttatcaa cgtcggcgag cgcaccaata
    29521 tcaccggctc ggccaagttc gcccggctga tccgtgagga caactacacc gaagccctcg
    29581 aagtcgccct gcagcaggtg gaagccggcg cccaggtgat cgacatcaac atggacgagg
    29641 gcatgctcga ttcgcagaag gccatggtca ccttcttgaa tctgatcgcc ggcgagccgg
    29701 atatttcccg cgtgccgatc atgatcgact cctctaagtg ggaagtgatc gaggccggtc
    29761 tcaagtgcat ccagggcaag ggcatcgtca actcgatctc aatgaaagaa ggcgtcgagc
    29821 agttcaaaca ccacgccaag ctttgcaaac gctacggcgc ggccgtagtg gtgatggcct
    29881 tcgacgaagc cggccaggcc gacaccgccg cgcgcaaacg cgagatctgc cagcgcagct
    29941 acgacattct ggtcaatgaa gtgggcttcc cgccggaaga catcatcttc gacccgaaca
    30001 tcttcgccgt ggccactggc atcgaggagc acaacaacta cgcagtcgac tttatcgagg
    30061 cctgcgccta tatccgcgac cacctgcccc acgccctgtc gagcggcggt gtatccaacg
    30121 tgtcgttctc cttccgtggc aacaacccgg tgcgtgaagc cattcactcg gtgttcttgt
    30181 actacgcgat ccagaacggc ctgaccatgg gcatcgtcaa cgccggccag ttggaaatct
    30241 acgacgagat tcccaaagag ctgcgcgacg cggtggaaga cgtggtgctc aaccgccatg
    30301 aaggtgccac cgaagcgctg ctggcaattg ccgacaacta caagggcgac ggcagcgtca
    30361 aggaagtcga aaccgaagaa tggcgcacct ggaacgtggt cgaccgcctc aagcacgcac
    30421 tggtcaaggg catcaccagc catatcatcg aagacaccga ggaatgccgt cagcagtgca
    30481 cacggcctat cgaagtgatc gaaggcccac tgatggccgg tatgaacgtg gtcggcgacc
    30541 tgttcggcgc aggcaagatg ttcctgcccc aagtagtgaa atccgcccgc gtaatgaagc
    30601 aggcggtcgg ccatttgatc ccctttatcg agctggaaaa aggcgacaag ccggaagcca
    30661 agggcaagat cctaatggca acggtaaaag gcgatgtgca tgacatcggc aagaacattg
    30721 tcggtgtggt gctcggctgt aacggctatg acatcgtcga cctcggcgtg atggtgccgg
    30781 cggagaagat cttgcaaacc gcaattgccg agaagtgcga catcatcggc ctgtccgggc
    30841 tgatcacccc gtcgctggat gagatggtgc atgtcgcccg tgaaatgcag cgccaaggct
    30901 tcaccctgcc gctgatgatc ggcggcgcga ccacctcgaa agcccacacg gcggtaaaaa
    30961 tcgagcccaa gtaccagaac gacgcggtga tctacgtcac cgacgcctcg cgcgccgtcg
    31021 gcgtggccac acagttgctg tcgaaagaac tgaaagccga ttttgtcgag cgcacccgcc
    31081 tggactacat cgaagtacgc gagcgcaccc ccaac
//
LOCUS       DASRUM010000281         4676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_315732, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000281 DASRUM010000000
VERSION     DASRUM010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315732"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFF22_18840"
     CDS             <1..197
                     /locus_tag="VFF22_18840"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZX18911.1"
                     /translation="DDIRQRFAEIQYSAQPHDLSCTFSCGIAQLAPQLDGKLLSQQAD
                     LALYVAKHGGRNQVAIYQGD"
     gene            354..2318
                     /locus_tag="VFF22_18845"
     CDS             354..2318
                     /locus_tag="VFF22_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014336389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX18912.1"
                     /translation="MRLKSLTNLNTLLLVAVCVALAATLWWSQRALERPYLLMARYMS
                     LSQQFQSEAAQNISAYLESGDALRHSAALQSLDSLEQAMSDLPTQLAANLRPSLAELK
                     AFSANELLAAGKLSADPQGLLLQAEREMDAALEQLAQYADSGEASATANYRRPLFSAA
                     RHLTRLAHARDKLVRSGRSELASEVERELAALGKQAQTLSDLPLLGVADASQSSGDAF
                     ANLLGLDVSSESSQAEDRGIALKRDLSNLITRYPTELQRTRNLIEQRTALANSTRTQI
                     DQVQQALAALEPLVRAEHGRIQGEVQLILGLMIGLILLIALIIDTIQRRLTRVLEHLV
                     PALSAWAAGDFAHDIHLGSRTRELRDIEESLNRLRAYLVDLVGTIRRHAEQVVGSSRT
                     LADLSGGLHSGAERQAGDTAQIRDALGELQATIQQVAGDASQAADASRDANRALEQGQ
                     QVIGHSLTGLHSLVDEVQGNALAIERLAEETATIGNVLTVIRGIAEQTNLLALNAAIE
                     AARAGELGRGFAVVADEVRSLSQRTSGATAQIQELIGRLQQAALQSVQAMRTQVEHAE
                     ATASQAEAADGALDEIVAAIRTIASMAERIADATAQQSGAVSEIRGHSERIHTLGGDN
                     LQRIGEGRNQGEQLLQLGGQLHTAVQAFRV"
     gene            2446..4218
                     /gene="dsbD"
                     /locus_tag="VFF22_18850"
     CDS             2446..4218
                     /gene="dsbD"
                     /locus_tag="VFF22_18850"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HZX18913.1"
                     /translation="MHRLLSLLLLLVVLPASAGLFNKPPAPAQFGAPLNNSADFLPVR
                     EAFKLSLISSSSESVKLRFVAAEGYYLYRHRFSFSSEPADIRLDQAVLPAGQAKHDEY
                     FGDVEVYYEVLDVEVPVDNLDNRPFNLSVNYQGCADKGLCYPPETEVLAIGGGAATST
                     AAATATAVTPWSWTDLALFFLGGLALTFTPCVLPMLPILTGVVLRGQPGGMRSLVLSL
                     AYVLPMALSFAVLGALMGLFGAELNLQARLQSPWVLVPFAIFFALFGAASLGFLELRL
                     PASIDGPLQRLSQRLHGGTIFSAAALGVLSSVLVSPCVSAPLAGALLYISASGDALGG
                     GLKLLALGLGMGAPLVLFAVGGGALLPKSGPWMLTVRNLFGALLMAVAIWLLERVVPG
                     PVSLALWGLLAAGIALWLGVLELTPKTHHQKLAQLLGLPLLVYALVAWVGALQGQDDP
                     LRPLGRMVSSAEATQVQSGQWQTISTPAELDAALLAAKNNGKPLLLDWYADWCISCKV
                     IEREVLSAPEVGRQLGDYQLVRFDITESNPAQRALLDRYQLFGPPAILLFDAKGDEWL
                     DLRVVGEVDAATFAARLSTARERF"
     gene            4339..>4676
                     /locus_tag="VFF22_18855"
     CDS             4339..>4676
                     /locus_tag="VFF22_18855"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HZX18914.1"
                     /translation="MATLLVLHGPNLNLLGTREPGVYGAVTLEQINLDLERRAREAGH
                     HLLYLQSNAEYELIDRIHAAKGEGVDFILINPAAFTHTSVALRDALLAVSIPFIEVHL
                     SNVHKREAFR"
BASE COUNT          855 a         1594 c         1412 g          815 t
ORIGIN      
        1 tcgacgacat ccgccagcgc ttcgcggaaa tccagtattc agcgcagccc catgacctgt
       61 cctgtacctt cagctgcggc atcgcccaac tggcgccgca actggatggc aaactgctct
      121 cacagcaggc cgacctggcg ctgtatgtgg ccaagcacgg cgggcgcaac caggtggcga
      181 tctatcaggg cgactgaact cgccctgctc cccacggcca accacccggc agaaactgtg
      241 acgcagttgg cgtcgtcata ttacggtcat gaaaaagcaa taaattccga gacttagcag
      301 ccgctctgac gaatccgtcg gcggccggaa ccgttcgctg tcggttgagc cctatgcgcc
      361 tgaaatccct taccaatctc aacaccctgc tgctggtcgc cgtatgtgtg gcgctggccg
      421 ccaccctttg gtggtcgcag cgcgcccttg aacgccctta tttgctgatg gcgcgctata
      481 tgagcctgtc ccagcaattt cagagcgaag ccgcgcagaa catcagcgcc tacctggaaa
      541 gtggcgacgc cctgcgccac agcgccgccc tgcaatctct ggatagcctc gagcaggcca
      601 tgagcgatct gcccacgcag ctggccgcca acctgcgccc tagcctggcc gagctcaagg
      661 cctttagtgc caatgaactg ctagccgcgg gcaagctctc cgccgacccg cagggcctgc
      721 tgctgcaagc cgaacgggaa atggacgcag cattggagca gctcgcgcaa tacgccgaca
      781 gcggcgaagc cagcgcgacc gccaattacc gtaggccgct gtttagcgcg gcacgccatc
      841 tcacccgcct ggcccatgcc cgcgacaagc tggtgcgcag cggccgcagc gagctggcca
      901 gcgaagtcga gcgcgaactg gcagccctgg gcaaacaggc gcaaacgctt agtgacctgc
      961 cgttgctcgg cgtggccgat gccagccaat cgtccggcga cgcctttgcc aacctgctgg
     1021 ggctggatgt cagcagcgag agcagccaag ccgaagaccg tggcatcgcc ctcaaacgcg
     1081 acctgtccaa cctgatcacg cgctatccga ccgagctgca acgcacccgc aacctgatcg
     1141 aacaacgcac cgccctggcc aacagcacgc gcacgcagat cgaccaggtg cagcaagcgc
     1201 tggccgcgct ggaacccttg gtgcgtgccg aacacgggcg catccagggc gaagtgcagc
     1261 tgatcctcgg tctgatgatc ggcctgattc tgctgattgc actgatcatc gacaccatcc
     1321 agcgccgcct cacccgggta ctggagcacc tggttccagc tctctctgcc tgggccgccg
     1381 gcgacttcgc ccatgacatc cacctcggtt cacgcacccg cgagctgcgc gatatcgaag
     1441 agtcgctaaa ccgcctacgc gcctatctgg tcgacctggt cggcaccatt cgccggcacg
     1501 ccgaacaggt ggtcggttcc agtcgcaccc tggccgacct cagcggcggc ctgcacagcg
     1561 gcgccgaacg ccaggccggc gacaccgcgc aaatccgcga tgccctcggc gagctgcagg
     1621 ccaccatcca gcaggttgcc ggcgatgcca gccaggccgc cgatgccagt cgagatgcca
     1681 accgcgccct ggaacaaggc cagcaggtga ttggccacag ccttaccggc ctgcattcgc
     1741 tggtcgatga agtgcagggc aacgccctgg ccatcgagcg cctggccgag gaaaccgcca
     1801 ccatcggcaa tgtgctgacg gtgattcgcg gcatcgccga gcagaccaat ctactcgccc
     1861 tgaatgccgc catcgaagca gcaagggccg gcgagctggg ccgcggcttt gccgtggtcg
     1921 ccgatgaagt gcgctcgctg tcgcaacgca ccagcggcgc caccgcacag attcaggaac
     1981 tgatcggccg cctgcaacag gccgccctgc aatcagtgca ggccatgcgt acccaggtcg
     2041 aacacgccga agccaccgcc agccaggccg aggccgccga tggcgcgctg gatgaaatcg
     2101 tcgcggcgat ccgcaccatc gccagcatgg ccgagcggat tgccgacgcc acggcgcaac
     2161 aaagtggcgc ggtcagcgag attcgtgggc acagcgaacg cattcacact ctgggcggcg
     2221 acaacctgca gcgcatcggc gagggccgca atcagggcga gcagctgctg caacttggcg
     2281 gccagttgca tacggcggtg caggcgttcc gcgtctaaca ggctgctggc gcggcaatct
     2341 gtcgcgtacc atccagcccg tagacaagac cgcttacagc ctgggccgtg ccctgagccg
     2401 ctcgcctccg ctatgatgcg gcgattcccg catcacgagc ctgccatgca ccgtctactc
     2461 agcctgcttc tgcttctggt cgtactgccg gccagcgccg gcctgttcaa taagccgccc
     2521 gctcctgcgc aattcggcgc gccgctgaac aacagcgcgg atttcctgcc ggtgcgcgag
     2581 gcgttcaagc tgagcctgat cagcagctcc agcgagtcgg tgaaactgcg ctttgtcgcc
     2641 gccgaaggtt attacctgta tcggcaccgc ttcagcttta gcagcgagcc cgccgacatc
     2701 cgtctggacc aggccgtatt gccggcaggc caggccaaac atgacgagta cttcggcgat
     2761 gtagaggtgt attacgaagt gctggatgtc gaggtgccgg tagacaacct ggacaatcgc
     2821 cccttcaacc tcagcgtcaa ctaccagggc tgcgccgata aaggcctgtg ctacccgccg
     2881 gaaaccgagg tattggcaat tggcggtggc gctgcgacca gcactgcggc ggcgacggct
     2941 acagcggtga caccctggag ctggaccgac ctggcgctgt tcttcctcgg tggcctggcg
     3001 ctgaccttca ccccctgtgt gctgccgatg ttgccgatcc tcaccggcgt ggtgctgcgc
     3061 ggccagccgg gcggcatgcg cagcctggtg ctgtcgctgg cctacgtgct gccgatggcc
     3121 ctgagctttg ccgtcctcgg cgcgctgatg ggcctgttcg gcgcggaact taatctgcag
     3181 gcacgcctgc aatcgccctg ggtgctggtg ccctttgcaa tcttctttgc cctgttcggc
     3241 gcggccagcc tgggctttct tgaactgcgc ctgccggcct caattgacgg cccgctacag
     3301 cgcctgagcc agcgcctgca cggcggcacg attttcagcg cggcagcgct tggcgtgctc
     3361 tccagcgtat tggtatctcc ctgcgtttcc gcgccactgg caggcgcgtt gctgtatatc
     3421 agcgccagcg gcgacgcact cggcggtggc cttaagctgc tggcgctagg cctcggcatg
     3481 ggcgcgccac tggttctgtt tgccgtcggt ggcggcgcct tactgccgaa atccggcccc
     3541 tggatgctca cggtgcgcaa cctgttcggc gcactgctga tggcggtggc gatctggctg
     3601 ctcgaacgcg tcgtacctgg cccggtgagc ctggcactgt ggggcctgct ggccgcaggc
     3661 atcgcactgt ggctgggcgt gctggaactg acaccgaaaa cccatcacca aaagctcgcg
     3721 caactactcg gcctgccgct gctggtctac gccctggtgg cctgggtcgg cgcgctacaa
     3781 ggtcaggatg atccactgcg gccactcggg cgcatggtca gcagcgccga agccactcag
     3841 gtgcaatccg gccaatggca aaccatcagc acacccgccg agctggatgc cgccttgctg
     3901 gctgcaaaga acaacggcaa gccgctgctg ctcgactggt atgccgactg gtgcatcagt
     3961 tgcaaggtga tcgagcgtga agtgctcagc gcgccggaag tcggccgcca actgggcgac
     4021 tatcaactgg tgcgtttcga cattaccgaa agcaaccccg cccagcgcgc cctgctcgac
     4081 cgctatcagt tgttcggccc gccggcgatt ctgctgttcg acgccaaggg tgacgaatgg
     4141 ctggatctac gtgtcgtagg tgaggtggat gccgcgacct ttgctgcccg cctgagcacg
     4201 gcgcgggaac gtttttaacg ccccgagtca tatatttgcc gcaatctgcg gtcatcgtgt
     4261 cgactattgc tgagaactgg acagccccgc gctgtcgcgg catagtcctg cgcagtagaa
     4321 aaacaaggaa gcaccgccat ggcgacccta ctggtattgc acggccccaa cctcaaccta
     4381 ctcggcaccc gcgagccggg agtctacggc gccgtcaccc tggagcagat caacctcgac
     4441 ctggagcgcc gcgcgcgcga ggcaggccac catctgctgt atctgcagag caatgccgag
     4501 tacgagctga ttgaccgtat tcacgccgca aaaggcgaag gtgtcgactt tatcctgatc
     4561 aatcctgccg cttttactca caccagcgtc gcattacgtg acgcattgct ggcagtgagc
     4621 atcccattca tcgaagtgca tttgtccaac gtgcacaaac gcgaagcttt ccgcca
//
LOCUS       DASRUM010000282         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1804803, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000282 DASRUM010000000
VERSION     DASRUM010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804803"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1825)
                     /gene="nuoL"
                     /locus_tag="VFF22_18860"
     CDS             complement(<1..1825)
                     /gene="nuoL"
                     /locus_tag="VFF22_18860"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HZX18915.1"
                     /translation="MNLLFLTCVFPLLGWFLLAFSRGRFSENLSALIGVGSVGLSALT
                     AAWVIVQFNLNPPENGVFTQVLWQWMSVAGFAPSFTLYLDGLSLTMLGVVTGVGFLIH
                     LFASWYMRGEEGYSRFFAYTNLFIFSMLLLVLGDNLLVLYFGWEGVGLCSYLLIGFYF
                     KHAANGNAALKAFIVTRIGDVFMAIGLFILFLHLGTLNIQELMVLAPQKYVAGDSWLW
                     IATLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFL
                     LTPEILELVGIVGGVTLVLAGFAALVQTDIKRILAYSTMSQIGYMFLALGVGAWDAAI
                     FHLMTHAFFKALLFLASGAVINACHHEQNIFKMGGLWKKLPLVYASFIVGGAALAALP
                     LITAGFYSKDEILWEAFASGHQWLLYAGLTGAFLTSLYTFRLIFIAFHGEQKTEAHAG
                     HGVAHWLPLVTLIVLSTFIGALISPPLAGVLPQSVGHAGGDAKHGLEIASGAIAIIGI
                     VLAALLFLGKRSFATAVAQSAPGRFLSAWWFAAWGFDWLYDKLFVQPYLLVCRLLGHD
                     PIDRSIGLIPRLARGGNALLASSETGQVRWYATSIAGGAVLV"
     gene            complement(1822..2130)
                     /gene="nuoK"
                     /locus_tag="VFF22_18865"
     CDS             complement(1822..2130)
                     /gene="nuoK"
                     /locus_tag="VFF22_18865"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HZX18916.1"
                     /translation="MNAIPLEHGLALASVLFSLGLIGLMVRRNILFVLMSLEVMMNAT
                     ALAFVVAGSRWAQPDGQVMFILVITLAAAEASIGLAILLQLYRRFNTLDVDAASEMRG
                     "
     gene            complement(2127..>2548)
                     /gene="nuoJ"
                     /locus_tag="VFF22_18870"
     CDS             complement(2127..>2548)
                     /gene="nuoJ"
                     /locus_tag="VFF22_18870"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HZX18917.1"
                     /translation="YLILSLLAVAMTFFALGAPFAGALEIIVYAGAIMVLFVFVVMML
                     NLGPAAVEQERQWLTPGIWTGPAILSALLLGQLLYVLFQSPSGAALCLQTIDAKAVGI
                     ALYGPYLLAVELASMLLLAALVAAYHLGRHDAKEPTA"
BASE COUNT          606 a          807 c          783 g          352 t
ORIGIN      
        1 gcaccagcac ggcgcccccg gctatcgatg tggcgtacca acgcacctgg ccggtctcgc
       61 tgctggccag caaggcgttg ccgccacgcg ccagacgcgg aatcaggcca atgctgcggt
      121 cgatcgggtc gtgcccgagc aggcggcaga ccagcagata cggctgcaca aacagcttgt
      181 cgtagagcca atcgaagccc caggcggcga accaccaggc cgagaggaaa cgccccggtg
      241 cactctgcgc taccgctgtg gcaaagctgc gcttgccgag aaacagcaac gccgccagca
      301 cgatgccaat gatcgcgatg gcccccgagg cgatttccag gccgtgcttg gcgtcaccgc
      361 cggcatgccc gacgctctgc ggcaacacgc cggccagcgg tgggctgatc agcgcaccaa
      421 taaaggtcga cagcacaatc agggtcacca gcggcagcca gtgagccacg ccatggccgg
      481 catgggcttc ggtcttctgc tcgccatgga aggcgataaa gatcagccgg aaggtgtaga
      541 gcgaggtcag gaaggcgccc gtcaggccgg catagagcaa ccactgatga ccactggcaa
      601 aggcttccca gaggatttca tccttcgagt agaaaccggc ggtaatcagc ggcaacgccg
      661 ccagcgctgc gccgccgacg ataaagctgg cgtagaccaa cggcagtttc ttccacagac
      721 cgcccatctt gaagatgttc tgttcgtggt ggcaggcgtt gatcaccgca ccggatgcga
      781 ggaacagcag ggccttgaag aaggcgtggg tcatcaggtg gaagatcgcc gcgtcccagg
      841 cgccaacgcc cagggcgagg aacatatagc cgatctggct catggtcgag taggcgagga
      901 tgcgcttgat gtcggtctgc accaacgcag cgaaaccggc cagcaccagg gtcacgccgc
      961 cgacgatgcc aaccagttcg agaatctccg gggtcagcag gaacaggccg tgggtccgcg
     1021 cgatcaggta gacaccggca gtaaccatgg tcgctgcgtg gatcagtgcg gaaaccggag
     1081 tcggaccggc catcgcatcg gccagccagg tttgcagcgg cagctgagca gatttaccca
     1141 ccgcgccgcc gagcagcatc agtgtggcga tccacagcca gctatcgcca gctacgtact
     1201 tctgcggtgc cagcaccatc agctcctgaa tattcagggt gcccagatgg aggaacagga
     1261 taaacaggcc gatggccata aacacgtcgc cgatacgggt gacgataaag gccttgagcg
     1321 ccgcgttacc gttggcggcg tgcttgaagt agaagccgat cagcaggtag gagcacagcc
     1381 ccacgccttc ccagccgaag tacagcacca gcaggttatc gcccagcacc agcagcagca
     1441 tgctgaagat aaacaggttg gtgtaggcga agaagcgcga gtagccctct tcaccgcgca
     1501 tataccagct ggcgaacagg tggatcagga aaccgacccc ggtgaccaca ccaagcatgg
     1561 tcagcgacag accgtccagg tacagggtga agctcggcgc aaaaccggcc acgctcatcc
     1621 actgccacaa cacctgggtg aacacgccgt tttccggcgg attaaggttg aactgcacga
     1681 tcacccaggc cgccgtcagc gcggacaggc ccaccgagcc aacaccgatc agcgcagaaa
     1741 ggttctcgga gaaacgtccg cgcgagaagg ccagaagaaa ccagcccagc aacggaaaca
     1801 cacaggtcag gaatagaagg ttcatccgcg catctcgctg gcagcgtcga catcgagggt
     1861 gttgaagcgg cgatacagct gcaacaggat cgccaggcca atactggcct cggcggcggc
     1921 cagagtgatc accaggataa acatcacctg gccgtcaggc tgggcccaac ggctgcccgc
     1981 caccacaaag gccagggcgg tggcattcat catcacttcc aggctcatca gcacaaacag
     2041 gatattgcgc cgcaccatca ggccgatcag gccgaggctg aacagcacgc tggccaacgc
     2101 caggccgtgc tccaggggaa tcgcattcat gccgttggct ccttggcatc gtgacggccg
     2161 aggtgatagg ccgcgaccag cgcagcgagc agcagcatcg aggccagctc caccgccagc
     2221 aggtaaggcc cgtacagggc gataccgacg gccttggcat caatcgtctg caaacagagg
     2281 gccgcgccac tgggcgattg gaacaacaca taaagcaatt gccccagcaa caacgccgag
     2341 agaatcgccg ggccggtcca gatgccaggc gtcagccact ggcgttcctg ttcgaccgcc
     2401 gccgggccga ggttaagcat catcaccaca aacacgaaca gcaccatgat cgcgccggcg
     2461 tagacgatga tctccagcgc gccggcaaag ggtgcgccga gggcgaagaa ggtcatcgcc
     2521 accgccagca gcgagagaat cagataca
//
LOCUS       DASRUM010000283         5547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_799934, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000283 DASRUM010000000
VERSION     DASRUM010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799934"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VFF22_18875"
     CDS             <1..774
                     /locus_tag="VFF22_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003407454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase (NAD(P)H)"
                     /protein_id="HZX18918.1"
                     /translation="YDLYTKITGGQRIDLFGAQLHELPDIWAELIAAGFETGHAYGKS
                     LRTVKSCVGSTWCRYGVQDSVAMALLLEDRYKGLRSPHKIKFAVSGCTRECAEAQSKD
                     VGVIATENGWNLYVAGNGGMRPRHAELFATDLDDATLIRYIDRFLMFYVRTADRLQRT
                     SVWRESLECGLDYLKEVIIDDSLGLGAELEAQMQLVIDRYECEWANALKDPEKLKRFR
                     TFVNQQGGDPDIQFVEERGQRRPVRADELALIPLFEEVV"
     gene            774..1151
                     /gene="nirD"
                     /locus_tag="VFF22_18880"
     CDS             774..1151
                     /gene="nirD"
                     /locus_tag="VFF22_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HZX18919.1"
                     /translation="MSQSSATATVEQTSVQWRALCSRADLVANSGVVAWVDGAQVALF
                     YLPDETSGKQLYAVDNHDPKSGANVIGRGIVGSLAGDLVIAAPLYKQHYRLEDGSCLE
                     YPEQQLRTWPVRLQGDRVEIGLS"
     gene            1309..2067
                     /gene="modA"
                     /locus_tag="VFF22_18885"
     CDS             1309..2067
                     /gene="modA"
                     /locus_tag="VFF22_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZX18920.1"
                     /translation="MHRRLSQALFTLCISLSCIAVTQAGDVQLAVAANFSAPIPAIAR
                     GFEEATGHRLVTAYGATGQLYAQIKNGAPFEVFLAADELTPAKLESEGEVVAGSRFTY
                     AIGALALWSAQVGYVDERGAVLKANTFQHLAIANPKTAPYGLAAAQVLAKLGLSQAVA
                     PKLVEGQSIAQTVQFVASGNAELGFVALSQVYKDGLLTGGSAWQVPAELHAPIRQDAV
                     ILKKGEHNPAAAAFMDYMKGEKAAAIIKSYGYTL"
     gene            2080..2772
                     /gene="modB"
                     /locus_tag="VFF22_18890"
     CDS             2080..2772
                     /gene="modB"
                     /locus_tag="VFF22_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HZX18921.1"
                     /translation="MPLTKADLAAVLLTFELASLTTLLLLLIGTPIAWWLARTDSRWK
                     QPIGAIVALPLVLPPTVIGFYLLVSMGPHGFIGQLTQSLGLGTLTFTFTGLVIGSVFY
                     SLPFVVQPLQNAFEAIGRAPLEAAATLRAGPWDAFFTVVLPLAKPGFVTAAILGFAHT
                     VGEFGVVLMIGGNIPGKTQVASVQIYNHVETMQYAQAHWLAGGMVLFSFIVLLALYSG
                     RSSGRSSTRVWQ"
     gene            2769..3911
                     /gene="modC"
                     /locus_tag="VFF22_18895"
     CDS             2769..3911
                     /gene="modC"
                     /locus_tag="VFF22_18895"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HZX18922.1"
                     /translation="MIWPLARRNPAIRTVAGVGEIHARFKVAHAGFTLDVELNLPGRG
                     VSALFGHSGSGKTSCLRCFAGLDQPSEGYLQVNGELWQDSTQGIFVPAHQRAVGYVFQ
                     DANLFAHITVRRNLQYGLKRIPAAQRRIALEQAVELLGIEHLLERLPGKLSGGEQQRV
                     GIARALLTSPRLLLLDEPLASLDLKRKQEVLPYLERLHQELEIPIVYVSHSPDEVARL
                     ADHLVLLEDGKVSASGPLKETLLRADLPFVFEDDAEAVVDGQVSGHDPAYGLLSLQLP
                     GSAINLRLPHGPLPLGQSVRIKVKARDVSLSLQQAEDSSVLNLLPARVLDWINIAEQA
                     HVLVRVQVAGEQLIARITRYSFDRLQIQREQLLWAQVKSVSLLSAG"
     gene            4088..4831
                     /locus_tag="VFF22_18900"
     CDS             4088..4831
                     /locus_tag="VFF22_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18923.1"
                     /translation="MRSTTLYRPLLLAVVGLLAGLSPLTFAQAVTPQEQVQVQASRVT
                     SSLMLLRGEGFQKKHQDALETDLQALAAAVQSLPQSSDALRSAHQELVVQIRRGVAFG
                     PSEDDMPWRYPEELSKALLGVLEETRKLAGPDDSELSAKLEYLSVQYLSRAYFGNFET
                     AREQPDTYLGQDERKIVPSVDAQLAALDSKADPQVGKLKTRWDYLKAALVDLNSQSSA
                     LQSASGRPFAPITVYRHTRSLTTQWMAMY"
     gene            complement(4892..5416)
                     /locus_tag="VFF22_18905"
     CDS             complement(4892..5416)
                     /locus_tag="VFF22_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HZX18924.1"
                     /translation="MKTLVDHLAQYAAYHRDQRNILSHFIGIPLIVVAVAVLLSRPGF
                     ELLGLWLSPALLVALAAGVFYLRLDLRFGLLMALLLVLSLWAAAGLAVQSTAVWLSSG
                     IGLFVIGWIIQFVGHYYEGRKPAFVDDVMGLVIGPLFVAAEAAFLLGLRKEVEQAIVE
                     RAGPTCIREKKATA"
BASE COUNT         1047 a         1737 c         1723 g         1040 t
ORIGIN      
        1 tacgacctct acaccaagat caccggcggc cagcgcatcg acctgttcgg cgcgcagctg
       61 cacgaactgc cggatatctg ggccgagctg attgccgccg gcttcgagac cggccacgcc
      121 tacggcaagt cgctgcgcac cgtgaagagc tgtgtcggca gcacctggtg ccgctacggc
      181 gtgcaggaca gcgtggccat ggcactgctg ctggaggacc gctacaaggg cctgcgttcg
      241 ccgcacaaga tcaagttcgc ggtttccggc tgcacccgcg aatgcgccga ggcgcagagc
      301 aaggacgttg gcgtgatcgc caccgagaac ggctggaacc tctatgtggc cggcaacggt
      361 ggcatgcgcc cacgacatgc cgagctgttc gccaccgatc tggatgacgc caccctgatc
      421 cgctatatcg accgtttcct gatgttctac gtgcgcaccg ccgaccgcct gcaacgcacc
      481 tcggtgtggc gcgagtcgct ggaatgcggc ctggattacc tgaaggaagt gatcatcgac
      541 gacagcctgg gtctcggcgc cgaactggaa gcgcagatgc agctggtgat cgaccgctac
      601 gagtgcgaat gggccaacgc cctgaaggac ccggagaagc tcaaacgctt ccgcaccttc
      661 gtcaatcagc agggcggcga cccggatatc cagtttgtcg aagaacgcgg ccagcgccgc
      721 ccggtgcgcg ctgacgagct tgcccttatt ccgctattcg aggaggtcgt ctgatgagcc
      781 agtcgagcgc aaccgcaaca gtcgaacaaa ccagcgtgca gtggcgcgcc ctgtgcagcc
      841 gtgccgatct ggtggccaac tcaggcgtgg tcgcctgggt cgacggcgcc caggtggcgc
      901 tgttctatct gccagacgag accagcggca aacagctgta tgccgtggat aaccacgatc
      961 ccaagtccgg cgccaacgtt atcggtcgcg gcattgtcgg cagcctggcc ggcgacctgg
     1021 tcatcgccgc gccgctgtac aaacagcact accgcctgga agacggcagc tgtctggaat
     1081 atcccgagca gcagctgcgc acctggccgg tgcgcttgca gggtgaccgg gtggaaattg
     1141 gcctgagcta aagtctaagc gcggtaaaca tcgaccagcc cgcgaacagc acccttgccc
     1201 ggtgttgctc gcgggctttg tcgttcaatg gccgtccgta taggatggcc atgccgcggc
     1261 acggcaaaac ctgcactgcc ggtaatccat aagcgaagga acccgtgcat gcaccgccga
     1321 cttagccagg ccctgttcac gctctgcatc agcctgagct gcattgccgt tacccaggcc
     1381 ggggatgttc agttggcagt ggctgccaac ttcagcgcac ccattccggc tatcgctcgc
     1441 ggcttcgagg aggccacagg gcatcggctg gtgacggctt acggcgctac cgggcagctc
     1501 tatgcgcaga tcaagaacgg cgcacccttc gaagtatttc tcgccgccga tgagctcacc
     1561 ccggccaagt tggaaagcga aggtgaggtg gttgccggct cacggtttac ctatgccatt
     1621 ggcgccctgg ctttgtggtc ggcgcaggtc ggctatgtcg acgagcgcgg cgccgtgctc
     1681 aaagccaata cgtttcaaca cctggctatt gccaacccga aaaccgcccc ctatggcctg
     1741 gctgccgccc aagtgctagc caagctgggt ctgagccagg ccgttgcacc caagctggtc
     1801 gaagggcaga gcattgccca gaccgttcag tttgttgcca gtggcaatgc cgaactgggc
     1861 tttgtcgcgc tgtcgcaggt gtacaaggac ggtctgctca ccggaggctc ggcctggcag
     1921 gtgcctgctg aattgcatgc cccaattcgt caggatgcgg tgatcctcaa aaagggcgag
     1981 cacaacccag ccgctgcggc ttttatggat tacatgaagg gtgaaaaagc agctgcaatc
     2041 atcaagtcct acggctacac gctttaaagg gacgttgcga tgccactgac caaagccgat
     2101 ctcgccgccg tactgctgac atttgagctg gcatcgctga ctaccctgtt gctgctgctg
     2161 atcggtacgc cgattgcctg gtggctggcg cgcactgatt cacgctggaa gcagccgatt
     2221 ggcgccatcg tcgccctgcc gctggtgctg ccgccgacgg tgatcggctt ctacctgctg
     2281 gtgagcatgg ggccgcacgg ctttatcggc cagctgaccc agagcctcgg cctcggcacc
     2341 ctgacgttta cctttaccgg gctggtgatc ggttcggtgt tctattccct gccctttgtc
     2401 gtgcagccgc tgcagaacgc cttcgaggcg attggccgtg ccccgctgga ggcggcggcg
     2461 actctgcgtg ccgggccttg ggatgcgttc ttcacggtgg tgctgccgtt ggccaagccg
     2521 ggtttcgtta cggcggcgat tctcggcttc gcgcataccg tgggcgagtt cggcgtggtg
     2581 ctgatgattg gcggcaatat ccccggcaag acccaggtgg cgtcggtgca gatctacaac
     2641 catgtagaaa ccatgcagta cgcccaggcc cactggctgg ccggcggcat ggtgctgttc
     2701 tcatttatcg tgctgctggc gctctactct ggtcgaagca gcgggcgcag cagcacgcgg
     2761 gtgtggcaat gatctggcca ttggcgcggc gcaatccggc aattcgcaca gtcgccgggg
     2821 tgggcgaaat ccacgcgcgg ttcaaggtgg cgcacgccgg ttttactctg gatgttgagc
     2881 tgaacctgcc cggtcgcggc gtcagcgccc tgttcggtca ttccggctcg ggtaagacca
     2941 gctgcctgcg ttgctttgcc gggttggacc agcccagcga aggctacctg caggtcaacg
     3001 gcgagctgtg gcaggacagt acccagggca tttttgtccc ggcgcatcaa cgcgccgtgg
     3061 gttacgtgtt tcaggatgcc aacctgtttg cccacatcac ggtgcggcgc aatctgcagt
     3121 acgggctgaa acgcatcccc gccgcgcagc ggcggattgc cttggagcag gcagtcgagc
     3181 tgctcggtat agagcatctg ctagaacgct tgccgggcaa gctgtccggc ggcgagcagc
     3241 agcgcgtagg cattgcccgc gccctgctga ccagcccgcg tctgttgctg ctggatgagc
     3301 cgctggcgtc cctcgacctc aagcgcaaac aggaggtgct gccttatctg gagcgcctgc
     3361 atcaggagct ggaaattccg attgtctacg tcagtcactc gccagatgaa gtggcgcgtc
     3421 tggccgatca tctcgtgttg ctcgaggacg gtaaggtcag cgccagcggc ccgctaaagg
     3481 aaaccctgct gcgcgctgac ctgccgtttg tcttcgagga cgatgccgag gcggtggtcg
     3541 atggtcaggt cagcggccat gatccggcct acggactgct ctcgctgcaa ctgcccggca
     3601 gcgccatcaa cctacgcttg ccccacgggc cgctgccgct gggccagtcg gtgcggatca
     3661 aggtcaaggc gcgcgatgtc agcctgagcc tgcagcaggc cgaggacagc agcgtgctca
     3721 acctgttgcc agcgcgggtg ctggattgga tcaatatcgc cgagcaggcc catgtgctgg
     3781 tgcgcgtgca ggtggctggc gagcagctga tcgcccgtat tacccgctac tcctttgacc
     3841 gcctgcagat ccagcgcgag caattgctgt gggcgcaggt caagtcggtg tcactactga
     3901 gcgccggctg aggctagcgc gaacagattg cacggcctgc caagctggcc gaatcgacgc
     3961 atttttccga cgaaatcact ctttaggctg cttttatccc atattcagtg cagtaccctg
     4021 tgcgcgcgct tgtataagct gacctagttc agcactgctc acaaaaataa taaggagcac
     4081 cccacgtatg cgttctacaa cgctgtatcg cccgctattg ctcgcggttg tcgggctgtt
     4141 ggctggcctc tcgccgctga cctttgccca ggccgtaacc ccccaggaac aggtgcaggt
     4201 gcaggccagc cgggttacca gcagcctgat gctgttacgc ggtgagggtt tccaaaagaa
     4261 acaccaggac gccctggaaa ccgatctgca ggccctggcc gcagcggtgc aaagtctgcc
     4321 gcaaagcagt gatgctctgc gcagtgccca tcaggaactg gtggtgcaga ttcgtcgtgg
     4381 cgtggccttt ggcccgagcg aagatgacat gccttggcgc taccccgagg aactcagcaa
     4441 ggccctgctc ggcgtgcttg aggaaactcg caagctggcc ggcccggatg acagcgaact
     4501 gtccgccaag ctcgaatatc tctcggtgca gtaccttagc cgcgcctact tcggcaattt
     4561 cgaaaccgcc cgcgagcagc ccgacaccta cctcggtcag gacgaacgca agatcgtgcc
     4621 gtcagtagat gcacaactgg ccgcgctgga tagcaaggct gacccacagg tcggcaagct
     4681 gaaaacccgc tgggattacc ttaaggccgc gctggtcgac ctcaatagcc agagcagcgc
     4741 cttgcagagc gcctcgggcc ggccgtttgc gccgatcacc gtatatcgcc atacacgctc
     4801 gctaactacg cagtggatgg cgatgtacta ggactcgctc gtaagagcga agtcggtaca
     4861 aggccgccaa tcaatggcgg ccccgcagcg atcaagcagt ggctttcttc tcgcgtatac
     4921 aggtcgggcc agcgcgttcg acgatggcct gttcaacctc tttgcgcagg cccagcagga
     4981 aggccgcttc ggcggcgacg aacagtgggc ctatcaccag gcccatcacg tcatcgacaa
     5041 acgccggttt gcgcccctcg tagtaatggc cgacaaactg gatgatccag ccgatcacga
     5101 acaggccgat cccgctgctt aaccacaccg ccgtcgactg cacggccagg cccgctgccg
     5161 cccacaggct gagcactagc agcagcgcca tcagcaggcc gaaacgcagg tccaggcgca
     5221 ggtagaacac ccctgccgcc agggctacca gcagagcagg ggacagccac aggccgagta
     5281 gctcgaagcc ggggcgcgac agcagcacgg caaccgccac aacgatcagt ggaataccga
     5341 taaagtggct gagaatattg cgttggtcgc ggtgataggc cgcgtattga gccagatgat
     5401 caaccagggt tttcattatt attttcctta tgcaagacag gtggccagca tgaacccgca
     5461 tgcgtacgcc gctctgtcag ctagccgaca aagccggagc cgccgatgaa cgccgcccac
     5521 agctatcagg cgtgtttgcg tcagggg
//
LOCUS       DASRUM010000284         7641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1148122, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000284 DASRUM010000000
VERSION     DASRUM010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148122"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFF22_18910"
     CDS             <1..339
                     /locus_tag="VFF22_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZX18925.1"
                     /translation="GQGTFASKVEVAGDKDNVTREIDGGLQTVALSLPAIVTTDLRLN
                     EPRYASLPNIMKAKKKPLETVTPADLGVSTASTVKTLKVEAPATRSAGIKVKSVAELV
                     EKLKNEAKVI"
     gene            340..1269
                     /locus_tag="VFF22_18915"
                     /pseudo
     CDS             340..1269
                     /locus_tag="VFF22_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(1336..2265)
                     /locus_tag="VFF22_18920"
     CDS             complement(1336..2265)
                     /locus_tag="VFF22_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX18926.1"
                     /translation="MKKLIAALMLLLTGTAALLYLSPAALLTSIQLVERQRAGLSLKQ
                     ISVGDLSIHYYEGGPSSAQTILMVHGFAANKDNWLRFARHLSQDYRVIALDLPGFGAS
                     DKPAGSYDVGTQTERLASVIDALKIEQLHLIGNSMGGHISALYAARYPHRTRSLALLD
                     NAGITSPQPSELLQRLQRSEPNPLVVKSPEDFQRLLDFIFVHPPYLPESLKGYFAEQA
                     IANSAHYDQVFTHLIERYIPLEPELPKIQAPTLIIWGAKDRALHVSSVEVMQPLLSKP
                     SVVILADTGHAPMIERPQQTAEHYRAFLQSLQN"
     gene            2378..3205
                     /locus_tag="VFF22_18925"
     CDS             2378..3205
                     /locus_tag="VFF22_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX18927.1"
                     /translation="MLERGLYRSFLLLFAALLMPALAVSAGKCERLVATGNPEYPPYL
                     WRDPQNPQQLIGANADLLQHLAKELGVVVDIIYTGPWSRAQDEVRTGRVDLIAGAFLT
                     LPRLEVMDYVHPAFFFTPSVVWVHRGAEFPYAGWDDLRNRTGDTLVNNSFGQTFDAYA
                     KSNLTLEGVASLTQAFQKLLLGRTDYVLYERYPGQALADSLGMQDDLLVLDPPISSEG
                     LYLTLSHNSACNDAWLRGMLAKKMNEAVASGLPDVLLQRNLQRWKDQQLTPTGDAEQ"
     gene            3215..3571
                     /locus_tag="VFF22_18930"
     CDS             3215..3571
                     /locus_tag="VFF22_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016779967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HZX18928.1"
                     /translation="MIYRRICAALLPLMLSACASDPAPNEQLRLTEQALSQAKALGIS
                     AEQSASLRLAEEKLVLAQAAMQDEDYKQARVYAEQAELDARLAEAEHLTEKSQQQLAE
                     LSNAINRLRKQLGGLQ"
     gene            3568..4380
                     /locus_tag="VFF22_18935"
     CDS             3568..4380
                     /locus_tag="VFF22_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZX18929.1"
                     /translation="MKHAQQSLWAIFAAVLLTGCASSYSTSDQALQGAHAAFQQVKED
                     GDVLRSAPKDLIRAGESLAKADRLSSYWGSADDVSHYAYLSQQYSAIAGQKSTLNLNQ
                     ERAVKLELERQRLQLALREAKLLSVQDQSNWLEEQMVSLATIQSERGLVMTLGDVLFD
                     AGHSDLKASANRTVLKLVQFLQLNPRRIVRIEGYTDSRGDAQENLELSRARAQAVADV
                     LVDLGIDGKRIEVQGHGESFPVTENASAKGRAQNRRVEIVFSDEQGNLGSSR"
     gene            4613..6052
                     /locus_tag="VFF22_18940"
     CDS             4613..6052
                     /locus_tag="VFF22_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZX18930.1"
                     /translation="MTNLLLYQRIAQQLAEDIRRGVYQPGERVPSVRKMSAQLSVSHA
                     TVLQAYANLEDQGLIRARPQSGFYVHQTPALTAPTPDIARVERPGLVTRSSIINQVLT
                     ESRREGVFPLGAAVPHVDYLPVRALHQQLAKVTRFHSARAFSYMFSPGFEPLRRQVAI
                     RMRDAGVVVDPTEVVITHGCVDALQMSLRVLTKPGDLIAAESPTYYGLLQLADLLGLK
                     VIEIPSDPTTGISLEALQLAANQWPIKALVLTARLSNPLGGTIPEERQRQLLRLAGDY
                     DIQIIEDDIYGELMFEQGPTKALKSHDREGRVVYCSSFSKTLSPGVRIGWIVAGKYQD
                     EIQRLQTFSTHSACSVTQMAVAAYLENGGYDRHLRFIRQEYRKNLSAFQLAVQQYFPE
                     GTQMSRPKGGFILWVSLPARVNTKDLHVRALQQGISIAPGLIFSNTEQFNHCIRLNCG
                     IPWNREAERALMTLGMLAGQLCQESAASF"
     gene            6131..>7641
                     /locus_tag="VFF22_18945"
     CDS             6131..>7641
                     /locus_tag="VFF22_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZX18931.1"
                     /translation="MSAYPVLLTRPLLAGFAAGVLISLGACSAANPEAPATAAGSPQL
                     SDQAHKRERAELHERLAAAPAELQRKSQVVGGAVMPSVAYAPMPMSVQADALPPAYLG
                     KSRDENREQYQKLAGNPVHAVAETPVSTFSIDVDTGSYANVRRFLNGGQLPPQDAVRL
                     EELVNYFPYAYPLPKGDVPFGVHTELAATPWNPESRLLRIAIKASDLRVDELPPANLV
                     FLVDVSGSMDRRDGLPMVQSTLKLLVEQLRAQDKVSLVVYAGSSSVVLQPTFGSDKAK
                     IRAAIDQLTAGGSTAGESGIQLAYQQAQQGFIDGGINRILLATDGDFNVGISDFETLK
                     QLAADKRKTGVSLTTLGFGTDNYNEQLMEQLADAGDGNYAYIDNLREARKVLVDQLSS
                     TLATVARDVKIQVEFNPAQVSEYRLLGYENRALKREDFSNDKVDAGEIGAGHTVTALY
                     EVVPVGGKGWLEPLRYQQAAKVGSPAGELAWLRIRYKTPGQNVSKLLEQPIQAA"
BASE COUNT         1502 a         2242 c         2368 g         1529 t
ORIGIN      
        1 ggccagggca ccttcgcttc caaagtggaa gttgccggtg acaaggacaa cgtaacccgc
       61 gaaatcgacg gcggtctgca gaccgttgcg ttgagcctgc cggctatcgt gactaccgac
      121 ctgcgtctga acgagccgcg ctacgcgtcg ctgccgaaca tcatgaaagc caagaaaaag
      181 ccgctggaaa ctgttacccc ggctgatctg ggcgtttcca ccgcttccac cgtcaagact
      241 ctgaaagtcg aagcgcctgc tacccgcagc gccggcatca aggtcaagtc cgtggctgaa
      301 ctggtcgaga aactgaagaa cgaggcgaag gtaatctaaa tgactatcct ggttatcgct
      361 gaacatacca atgccgctct ggctcctgcc acgctgaaca ctgttgccgc tgccgcgaaa
      421 atcggtggtg acatccacgt tctggttgcc ggttccgctt gcggcgctgc tgccgaagct
      481 gccgccaaga ttgctggcgt ggctaaggta ctggttgccg acaacgccgc tttcgctcac
      541 cagctgccgg aaaacgtcgc gccgctggta gctgagctgg gtaagggcta cagccacatt
      601 ctggctgctg ctaccagcaa cggtaagaac atcctgccgc gcgttgctgc tgcgctggac
      661 gttgatcaga tctcgtaaat catcgctgtt gaaagcgctg acactttcaa gcgtccgatc
      721 tacgctggta acgccatcgc caccgtgcaa tcttccgctg ccgtcaaagt catcaccgtg
      781 cgtagcactg gttttgatcc ggttgctgct gaaggtggtt ctgctgccgt tgaagcagtt
      841 ggcggcgcag ctgacgctgg caaatcggct ttcgtcggtg aagaactggc caagtccgat
      901 cgtcctgaac tgaccgctgc taagatcgtc gtttccggcg gccgcggcat gcagaacggt
      961 gacaacttca agcacctgta cgcgctggcc gacaagctgg gcgctgctgt aggtgcctcg
     1021 cgcgccgcag tcgacgcagg cttcgtaccg aacgacatgc aggtcggtca gaccggcaag
     1081 atcgttgcgc ctcagctgta tatcgccgtc ggtatctccg gcgcgatcca gcacctggcc
     1141 ggtatgaaag actccaaggt gatcgttgcg atcaacaagg acgaagaagc gccgatcttc
     1201 caggtagccg attacggtct ggtagcggat ctgttcgaag cagtacctga gctggagaaa
     1261 ctggtttaag ccggtttccc tggtgcagaa aaaacccgct cactgagcgg gtttttttat
     1321 cgccagcctt ttgcgctagt tctgtagaga ttggagaaag gcgcggtaat gttcagcagt
     1381 ttgctggggg cgctcaatca ttggcgcatg gccggtatct gccaggataa ccacgctggg
     1441 tttgctcagc aggggctgca tgacctcgac gctggatacg tgcaatgccc gatcttttgc
     1501 gccccagatg atcagcgttg gtgcctggat cttcggcagc tccggttcca gcgggatata
     1561 gcgttcgatc aggtgggtga atacctggtc atagtgggcg ctgttggcga tggcctgctc
     1621 ggcgaaatag cccttgagcg attccggcag gtagggcgga tgcacgaaga tgaagtccag
     1681 caagcgctga aaatcctcgg ggcttttcac caccagcgga ttgggttcgc tgcgctgcag
     1741 cctttgcagc agctcgctag gctgcggcga tgtgatgccg gcattgtcga gtagtgccag
     1801 agagcgggtt ctgtgcggat agcgcgcggc atacagcgca ctgatatgcc cgcccatgga
     1861 gttgccgatc aggtgcagct gctcgatttt cagggcatca atcacgctcg ccaggcgctc
     1921 ggtctgggtg cctacatcat aactgccggc cggtttgtcg ctggcgccga agcctggcag
     1981 gtcgagggct ataacccgat aatcctggct gagatgccgg gcgaaacgca gccagttgtc
     2041 cttgttggcg gcaaagccat gcaccatcag gatggtttgt gcgctgcttg ggccgccctc
     2101 gtagtaatga atgctaagat cgcccacgct gatctgcttg agactcagcc cggcgcgctg
     2161 tcgctccacc agctgtatgc tcgtcaacaa ggcggcgggt gagaggtaga gcagggctgc
     2221 agtgccggtg agcagtagca ttaaggctgc gatcagtttt ttcatggata gtctttatcg
     2281 cgaattcgta tggatgcgta ttaagctagc atgattccag atgtctcccg atgtgtggcc
     2341 gcagacgccc atccaaggtt aagtcgagaa gtcgccaatg cttgagcgtg gtttatatag
     2401 gtcgttcctc ctgctgttcg cagcgctgct gatgccggct ttagccgttt ccgcaggcaa
     2461 gtgcgaacgc ctggtggcga ctggtaatcc ggagtatccg ccgtacctct ggcgtgaccc
     2521 gcagaatccc caacaactga tcggtgccaa tgccgacttg ttgcagcacc tggccaaaga
     2581 gctgggcgtt gtggtggaca tcatctatac cgggccctgg tcacgggcac aggatgaagt
     2641 cagaaccggt cgcgtcgacc tgattgccgg tgccttcctg accctgccgc gcctggaagt
     2701 catggattac gtgcatccag cgtttttctt caccccaagt gtggtgtggg tgcatcgcgg
     2761 tgccgagttc ccctatgccg gctgggatga cttgcgcaat cgcaccggcg ataccttggt
     2821 taacaacagt tttggccaga catttgatgc ctacgccaag agcaatctga ctctggaagg
     2881 cgttgccagc ctgactcagg cgttccagaa actcttgctg gggcgtaccg actatgtact
     2941 ttacgagcgt tatccggggc aggctctggc cgatagcctg ggtatgcagg acgatctgct
     3001 ggtgctcgat ccaccgatct ccagcgaagg tttgtacctg acgttgtcgc ataactcggc
     3061 ctgcaacgat gcgtggctgc gcggcatgct ggcgaagaaa atgaacgagg cagtcgccag
     3121 cggtttgccg gacgtcttgc tgcagcgcaa tctgcagcgc tggaaagatc aacagctcac
     3181 acccaccggt gatgctgaac agtagggaaa ttctgtgatt tatcgacgta tctgtgcggc
     3241 cttgctgccg ctcatgctca gcgcctgtgc atccgatccc gcgcccaatg agcagcttcg
     3301 cttgaccgaa caggcgctta gccaggccaa ggccctgggt atttctgccg agcaatcggc
     3361 ctcgctgcgc ctggctgagg aaaaactcgt cctggcgcaa gcagccatgc aggacgagga
     3421 ctacaagcag gcgcgggtgt acgccgaaca ggccgagctg gatgcacggc tggccgaagc
     3481 tgagcacctg accgaaaaaa gtcagcagca actggccgag ctgagcaacg cgatcaatcg
     3541 cttgcgcaaa cagctggggg gcttgcaatg aagcatgctc aacaaagcct gtgggcgatt
     3601 ttcgcggcgg tgctgttgac cggctgcgcc tcatcgtaca gcaccagcga ccaggctctg
     3661 cagggcgcac acgctgcctt ccagcaggtc aaggaagatg gcgatgtgct acgcagcgcg
     3721 cctaaagatc tgattcgcgc cggcgagtcg ctggccaagg ctgatcgcct gtcgagctac
     3781 tggggcagcg ccgatgatgt cagccactat gcctatctca gtcagcagta cagtgcgatt
     3841 gctgggcaga agagcacgct caacctgaat caggagcgcg cggtcaaact ggagctggaa
     3901 cgccagcggc tgcagttggc cctgcgtgag gccaagttgc tcagtgtgca ggatcagagc
     3961 aactggctcg aagagcagat ggtcagcctg gccaccatcc agagcgaacg cggtctggtg
     4021 atgaccttgg gtgatgtgct gttcgatgcc ggccacagcg acctcaaggc ttcagccaac
     4081 cgcacggtac tgaaactggt gcagttcctt cagctcaacc cacggcgcat cgtgcggatc
     4141 gagggctata ccgacagccg tggcgatgcc caggaaaacc tcgaactctc tcgcgctcgt
     4201 gcccaggcgg ttgccgatgt actggtcgat ctgggcatcg atggcaaacg catcgaagtg
     4261 cagggccatg gcgagagctt cccggtaacc gaaaatgcct cggccaaagg aagggcgcag
     4321 aatcgtcgag tagagatcgt gttctccgat gagcaaggca acctcggctc gtcacgctaa
     4381 gtttgcgccg ttaaaaagcc ccggtttgcc ggggcttttt attgcctggc attcagattg
     4441 gcgcagcctg cgggcagtcg ttagacatcg ccgcacagtc catacacctg taaattgaac
     4501 cgctacagtc ctggcgggtt ggcactgtat cgcccacaat tcagtgagct gtaccggtgc
     4561 aattgtaaaa gcccggttta ctgttacggt tcagtcaagc aggaacgcgg tcatgaccaa
     4621 tctgttgctt tatcagcgta ttgcccagca actggcggaa gacatccgcc gcggcgttta
     4681 ccagcccggt gagcgtgtgc cttcggtgcg caagatgagt gcgcagctca gcgtcagcca
     4741 cgcgacggta ttgcaggcct acgccaacct ggaagaccaa gggctgattc gcgcccgccc
     4801 gcagtctggc ttctacgtac accagacccc ggccctgacc gcaccgactc cggatatcgc
     4861 ccgggtagag cgccccggcc tggtgacgcg cagcagcatt atcaatcagg tgctcaccga
     4921 gtcgcgtcgt gagggcgtgt ttccccttgg cgcagcggtg ccgcatgtgg attacctgcc
     4981 ggtgcgggcg ttgcaccagc agctggccaa ggtcacccgt tttcacagtg cgcgggcctt
     5041 cagctatatg ttcagcccgg gcttcgagcc gctgcggcgc caggtggcga ttcgcatgcg
     5101 cgatgccggc gtggtggttg acccgactga agtggtgatc acccacggct gtgtggatgc
     5161 cctgcagatg agcctgcgcg tgctgaccaa gccgggtgat ctgatcgccg ccgagtcgcc
     5221 gacctattac ggcctgctgc agctggctga cctgctcggc cttaaagtca tcgagattcc
     5281 cagcgaccca accaccggta tcagcctgga agccctgcaa ctggcggcta accagtggcc
     5341 gatcaaggcg ctcgtgctga cggcgcggtt gagcaacccg ctgggtggca ccattcccga
     5401 agagcgtcag cgccaactgc tgcgtctggc tggtgattac gatatccaga tcatcgaaga
     5461 cgatatctac ggcgaactga tgttcgagca gggcccgacc aaggcgctca agtcccatga
     5521 ccgtgaaggg cgcgtggtgt attgctcgag cttctccaag accctctcac ccggtgtacg
     5581 catcggctgg attgtcgccg gcaaatacca ggatgaaatt cagcgcttgc agaccttcag
     5641 cacccactcg gcgtgcagcg tgacgcagat ggcggtagcg gcctatctgg agaacggcgg
     5701 ttacgaccgc cacctgcgct ttattcgcca ggagtaccgt aagaacctca gcgccttcca
     5761 gctggcggtg cagcagtact tccctgaagg tacgcagatg agccggccca agggcggctt
     5821 tattctctgg gtcagcctgc cagcgcgggt caataccaag gatctgcacg tacgcgccct
     5881 gcagcagggc atcagcattg cgccggggtt gatcttcagt aataccgagc agttcaacca
     5941 ctgcatccgc ctgaactgcg gcattccgtg gaaccgcgaa gccgagcgtg cgctgatgac
     6001 cctgggcatg ctggccggtc agctgtgtca ggaaagcgct gcgagttttt aacagcggct
     6061 taacccgatg tagccgaggg ctgcgtctac ctgagcgaat ggtttattcg atcgctcagg
     6121 agatgcgccc atgtctgctt accccgtatt gcttacccgt cctctgcttg ccggttttgc
     6181 cgcaggcgtc ctgatcagcc ttggcgcgtg cagcgccgcc aaccccgagg cgccggccac
     6241 cgcggctggc agcccgcagc tcagcgatca ggcccataag cgtgaacggg ccgagctgca
     6301 tgagcggctg gccgctgccc ccgctgaact gcaacgcaaa agccaggttg tgggcggggc
     6361 ggtgatgccc agcgtggcct atgcgccgat gccgatgtct gtgcaggccg atgccttgcc
     6421 gcctgcttac ttggggaaga gccgcgacga gaaccgcgag cagtatcaga aactggcggg
     6481 caacccggtg catgccgtgg ccgagacccc ggtgtcgacc tttagcatcg atgtggatac
     6541 cggcagctac gccaatgtgc ggcgttttct caatggcggg cagttgccgc cgcaggatgc
     6601 ggtgcgtctg gaggagctgg tcaactactt cccctacgcc tatccgctac cgaagggcga
     6661 tgtgccgttt ggcgtgcaca ctgagctggc ggccacgccg tggaaccccg agagccgcct
     6721 gctgcgtatc gccatcaagg cttcggatct gcgggtagac gagctgccgc cggccaatct
     6781 ggtgtttctg gtggatgtct ccggctcgat ggaccgccgc gatggcctgc ccatggtgca
     6841 aagcaccctg aagctgctgg tcgagcagct gcgcgcccag gacaaggtat cgctggtggt
     6901 ctatgccggc tcatccagcg tggtgctgca gccgaccttc ggcagtgata aagccaagat
     6961 ccgcgcggcc atcgatcagc ttacggcggg cggttcaacg gcgggcgagt cgggcattca
     7021 gctggcctac cagcaggcgc agcagggctt tattgacggt gggatcaacc gaatcctgtt
     7081 ggccaccgat ggcgacttca acgtcggtat cagcgatttt gaaacgctca agcagctggc
     7141 cgccgacaag cgcaagaccg gcgtatcgct gaccaccctg ggcttcggca ccgacaacta
     7201 caatgagcag ttgatggagc agttggccga cgctggcgac ggcaactacg cctatatcga
     7261 caacctgcgc gaggcgcgca aggtactggt tgaccagctc agctcaaccc tggcgacggt
     7321 tgcccgcgat gtgaagatcc aggtcgagtt caacccggcg caggttagcg agtaccgact
     7381 gctcggttac gagaaccgcg cgctgaagcg tgaggatttc agcaacgaca aggtcgatgc
     7441 cggtgaaatc ggcgcgggcc acacggtaac cgcgctgtat gaagtggtgc cggtgggcgg
     7501 caagggctgg ctggagccgc tgcgctatca acaagcggcc aaggtgggca gcccggctgg
     7561 cgaactggcc tggctgcgca ttcgctacaa gacgccaggg cagaatgtca gcaagttgct
     7621 ggaacaaccg attcaggccg c
//
LOCUS       DASRUM010000285        12890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2470919, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000285 DASRUM010000000
VERSION     DASRUM010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 12890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12890
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2470919"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..97
                     /locus_tag="VFF22_18950"
     CDS             <1..97
                     /locus_tag="VFF22_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017529766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HZX18932.1"
                     /translation="RRYGACFWDVDVHWHYPQPGSICVLEVVPAG"
     gene            344..1678
                     /locus_tag="VFF22_18955"
     CDS             344..1678
                     /locus_tag="VFF22_18955"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011059745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZX18933.1"
                     /translation="MKTSTYVPTENLVDLLLDAICVVDKRGRFVFVSAACQRIFGYSA
                     DEMIGKVMIEMVHPEDRTKTLQAAKEVMAGVNKPNFENRYLRKDGSTVHILWSARWSE
                     DDQVRIAVAHDITERKRSEALQTALYGISEAAHSAKDLLGLFQQVHQIVGELLPTISF
                     SVALYDEQNDELSFPYPAEQPPATTADTPQNAATLSAQVIRSGNTLLLSDSSQTQPAA
                     PHSWLGVPLRSHRGIIGALLVQSSASTRYSERDQELLQFVSTQVATAIERQQMLSRLQ
                     FMAQYDQLTQLPNRELLRDRLHTALARARREQSQVALLFLDLDKFKQVNDSLGHAAGD
                     QLLQGVAQRIQLCLREADTVARFAGDEFVVLLEDFHSADHASVVAEKIRQSLNHPFEL
                     CGQSQTILPSIGIALYPLHAQDEQQLLQHADNAMYRAKQKGGNRAYSALELE"
     gene            complement(1728..2507)
                     /gene="fpr"
                     /locus_tag="VFF22_18960"
     CDS             complement(1728..2507)
                     /gene="fpr"
                     /locus_tag="VFF22_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-NADP reductase"
                     /protein_id="HZX18934.1"
                     /translation="MSNLNSERILSVHHWNDTLFSFKCTRDPGLRFENGQFVMIGLQQ
                     DNGRPLMRAYSIASPNWEEHLEFFSIKVPDGPLTSQLQHLKEGDEVIISKKPTGTLVL
                     DDLKPGKHLYLLSTGTGLAPFMSVIQDPETYERFEKVILVHGVRYVNEVAYREFITEH
                     LPQNEFFGDALKDKLIYYPTVTREPFENQGRLTDLMRSGKLFEDIGLPPINPQDDRAM
                     ICGSPSMLDETSEVLNEFGLQISPRMREPGDYLIERAFVEK"
     gene            2631..3557
                     /locus_tag="VFF22_18965"
     CDS             2631..3557
                     /locus_tag="VFF22_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX18935.1"
                     /translation="MRFTLRQLQVFVAVAQHESVSRAADSLALSQSATSTSLSELERQ
                     TDCQLFDRAGKRLCLNSLGLQLLPEAVALLDQAKEIERLLGGKSGYGSLNVGATLTVG
                     NYLATLLIGSFMQRHPECRVKLQVHNTAYVVQQIAHYELDMGMIEGDCQHPDIEVQPW
                     VEDELVVFCAPQHALAQRGEASLEELTREAWILREQGSGTRLTFDQAMRHHPSSLNIR
                     LELEHTEAIKRAVESGLGIGCISRLALRDAFRRGSLVAVETPELDLRRQFYFIWHKQK
                     YQTAAMREFIELCRALTAGVTRSDQIVLPTIA"
     gene            complement(3569..3934)
                     /locus_tag="VFF22_18970"
     CDS             complement(3569..3934)
                     /locus_tag="VFF22_18970"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020304558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HZX18936.1"
                     /translation="MSPFKGQTGLKRVLNATGYSLAGLRAAFTGEAAFRQLLLINLVL
                     VPLAFYLDVSRVERALMVAVCLLALIVELLNSAVEAAIDRISLELHPLSKNAKDMGSA
                     AQFTALSLIALVWAIILLG"
     gene            complement(3993..4643)
                     /gene="erdR"
                     /locus_tag="VFF22_18975"
     CDS             complement(3993..4643)
                     /gene="erdR"
                     /locus_tag="VFF22_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ErdR"
                     /protein_id="HZX18937.1"
                     /translation="MAAFEILIADDHPLFRSALQQALTLGLGPDVRLVEAASIAELEA
                     CLAQKTDWDLVLLDLNMPGAYGFSGLVLLRGQYPHIPVVMISAQEEPSIVARSREFGA
                     SGFIPKSSPLETIQQAVRLVLDGEVWWPQQTQEAIALSAEAKAASAGLASLTPQQFRV
                     LTMVCDGLLNKQIAYELSVSEATVKAHVTAIFRKLGVRTRTQAALLLQQLESIPAS"
     gene            4843..5730
                     /locus_tag="VFF22_18980"
     CDS             4843..5730
                     /locus_tag="VFF22_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZX18938.1"
                     /translation="MQSHALRADLLMLLTAMIWGSAFVAQRLGMDSIGPFLYSGLRFA
                     LAALILLPVLRLLEGRSSSATVAPLNRQLLRGGVLMGLALALGINLQQVGLLFTSVTN
                     SGFITGLYVIIVPLLGLFIGHKTGLGIWLGACLAVVGMFLLSVGEGFTVASGDWLQLA
                     GAFVWGVHVLLVGFFAGRHDPLRLALVQFITCAVISLVLAVIFEEIQLQAIIAAGPAI
                     LYGGIFGVAIGFTLQVVAQKDAIASHAAIILSLEAVFAAIAGAWLLGESLELRGYFGC
                     ALMFAGMLLAQLWPKKLAH"
     gene            complement(5734..6444)
                     /locus_tag="VFF22_18985"
     CDS             complement(5734..6444)
                     /locus_tag="VFF22_18985"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011059584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HZX18939.1"
                     /translation="MSHAVARLRAARIARSAKPFLARGSRSVRCDDCRLVMTHCLCAW
                     RPRIAATSAVCLLMHDVEALKPSNTGWLIADVLADTSAFGWSRVEVDAGLPALLRDPQ
                     WQPYLVFPGEYVAAERVVCEVLPAPGKRPLFVLLDATWSEARKMFRKSPYLDSLPVLS
                     LQAEQLSRYKLRRSKRDDHFCTAEVAALCLELAGDQRAADGLNAYLDVFSQHYLAAKA
                     PRAVPLDDPMHDRLREFL"
     gene            complement(6613..8310)
                     /locus_tag="VFF22_18990"
     CDS             complement(6613..8310)
                     /locus_tag="VFF22_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HZX18940.1"
                     /translation="MTRITRYGSLVFFAVAPLTTPYGMVQASGYHFGSQSVSAQGSAH
                     SNGAEAADASTIFYNPAGLARLKGTQVTSGISILLPDGKYQDTGSADVFGQPVSGDAG
                     SFLPEAAAAPNLYFSHEVNYWLSVGLGIFTPFGAKLDYKEDWAGRYGIQSASLETVNF
                     NPSIAIRFNEHHSIGFGVSAQYIKSIQRGAADVQGASRQLAGQFVDANYDSTRNAADP
                     ILGPIVGLIAGVPVPDEITSCRGVADRDGFVDCVANNFADNVQGDGYFRVKGDDWGFG
                     WNIGYLWEPTESTRFGVAYRSNIRHTLDGESKWSFASVQGAVPSPDTSLDGGFTIPIV
                     DIYVPPTEALQQLFDEGNWVNPGDFAGERLHPNSKAKTAIDTPEMLSFNAFHQLNEQI
                     ALMADLTFTRHSRLDEVRIGLDQVSGYPYFNGVTEGDLAVKQDWKDTYKLSLGMNYQY
                     DDKLLLRTGVAYDKSPVSSTQLRHPAFPDADRYWLSLGANYRVTQDTSVDLAYSFVKF
                     ATGKMDYTDNCSPAGWVPGSGGLYQDSGVRCTGNGGNYSGEYETHIHFIGLALNHRF"
     gene            8636..9037
                     /locus_tag="VFF22_18995"
     CDS             8636..9037
                     /locus_tag="VFF22_18995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing-regulated virulence factor family
                     protein"
                     /protein_id="HZX18941.1"
                     /translation="MLRFIVPALAFGLLLPSAHAASLKDYELTKTLEKVARESSVGTP
                     RAINEDILDQGYTVDGSELVNHLSVRSEHAAQMRGNPDVVRAQLANSVCRNSGYRQLL
                     LRGAALRYEFSEYKSNRPVTTERFSKADCGL"
     gene            complement(9041..9241)
                     /locus_tag="VFF22_19000"
     CDS             complement(9041..9241)
                     /locus_tag="VFF22_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18942.1"
                     /translation="MPKLTLDLDVDLYRLLQQAARNNQISLEEECLQRLEGAARRSRY
                     MHALLADLRADDEQRRAQQGGG"
     gene            complement(9277..11679)
                     /locus_tag="VFF22_19005"
     CDS             complement(9277..11679)
                     /locus_tag="VFF22_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015502494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose 5-phosphate 3-epimerase"
                     /protein_id="HZX18943.1"
                     /translation="MTQLFPSASELAEHARLEPTFAAWQRGYGPLQHSPQTCAAVYRM
                     AHQLVQAGAQADLPSVYRHFRALDRLTAAGLWLVVHMTYARRVRLDGVALAAEDFKVS
                     PEGHTGGALNMVPAYAGYLALNNLTGETRGWLMGQGHCVAAIEALNLLTANQHPEQAE
                     RYPCDEAGMSQLVADFYSYVQNAAGQVSAPLGSHVNPHTAGGIAEGGYLGFAELQYAH
                     LPLPGEKLVAFLSDGAAEEQRGSDWMPRWWRAEDCGVALPLMIANGRRIEQRTELATP
                     AGLDNFREHLRHCGFDPVSFDGRDPAAFVCALWDMEQRMSRRVQELRDGVLNYPLPMP
                     YGIAETLKGFGFYGAGSNAAHNLPLPANPHTDEAARELFNQHAAQLWVAPDELSGACA
                     LFANRGSRTLERDNPLAQRHPALPKLPELQYQDSACSPMLALDRFFVDLVQANPLLRP
                     RIGNPDELASNRLGGVLKALKHRVTAPESELEALDGAIITALNEEAVVSACLANQGGL
                     NLVASYEAFCVKMLGAVRQRIIFARQQKEAGRPAGWLGWPLVATSHTWENGKNQQSHQ
                     DTTFCEALLGEMSDMMRVLFPADHNSVLALLPGIYQARGQIACMVIAKRERPCVFTRE
                     QAEQLAQDGAVVLAEQQGEQPLLLIATGSYQLAEMQRAAVRLSAAGCAWRLVYLQEPG
                     RFRAARDTWEQAVIVDAERVERLFPAASVQRVLLTHMRPEVARGHLWPLLGDARHSRV
                     LGYRNRGGTLDEAGMLFANQASWAHVLEAAAELLEKPLGELLSAEEQAAVQGRGEPRC
                     LR"
     gene            11809..>12890
                     /locus_tag="VFF22_19010"
     CDS             11809..>12890
                     /locus_tag="VFF22_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZX18944.1"
                     /translation="MALLNFLGAIQQVTGSCYLIESRDGARVLLDCGMRQGRREEEDG
                     NRAPFAFDPHSLDAVVISHAHIDHTGLLPKLTAAGYRGPIFATDATCELMELMLLDAA
                     FLQEKDAEWENKWRARMGKVPISPLYTTADAEQALSQRRPMIYGESREVAKGVQVTFH
                     DAGHILGSAIVELEVQDHHLTRRLVFSGDLGNTCSPLMHDPAILKRADVVLLESTYGD
                     RDHRCSSDTLDELAEILQQAHRDGGNVLIPSFAVGRTQDLIYYLGRFYQEGRLPQQAV
                     FLDSPMAIRANAIYSRFQNQFAAEDRAALAAKKVKRIEDWLPILRCTPSPEESMAINR
                     IKSGAIIIAGSGMCTGGRIVHHFKHNL"
BASE COUNT         2683 a         4062 c         3779 g         2366 t
ORIGIN      
        1 acgccgctat ggcgcgtgct tctgggatgt cgatgtgcac tggcactacc cgcagcctgg
       61 cagcatctgc gtgctggaag tcgtgccggc cggttgacct ggttcccggc agcgcttcag
      121 cagcgtgtaa tagtcggcac tacgctgcca acacagcgtt ctatactgag tggataccgg
      181 cgcagtgatg acctcagcaa gcgctcggct gagtcatgca ggcggcaaac ggacgttcac
      241 tgttaaacgc cttgcaggta tggtgagtgc atgggccata ctgccaaaac gccagcatcc
      301 ggcccggcct gcccgccatc acgcaccgac gtattgtgag cgtatgaaaa ccagcaccta
      361 cgtgccgacc gagaaccttg tcgatctgct gctcgacgcc atctgcgtag tcgacaaacg
      421 tggacgcttc gtttttgtca gcgccgcatg ccagcgcatt ttcggctaca gcgcagatga
      481 gatgatcggc aaggtgatga tcgagatggt gcatcccgaa gaccgtacaa aaaccctgca
      541 ggccgccaag gaagtgatgg ccggggtaaa caaacctaac ttcgagaacc gctacctgcg
      601 caaggatggc agcaccgtgc atatcctctg gtccgcgcgc tggtccgagg atgatcaggt
      661 acgtatcgcc gtcgcccatg acatcaccga acgtaagcgc agcgaagccc tgcagaccgc
      721 gctgtacggc atatccgaag cagcccattc ggccaaggac ctgctcggac tgtttcagca
      781 ggtgcaccag atcgttggcg aactgctgcc aaccatcagc ttttcggtgg cgttgtacga
      841 tgagcagaac gacgagctga gcttccccta tcccgccgaa cagccgccag caacgaccgc
      901 cgacaccccg cagaatgccg ccacgctgag tgcgcaggtg attcgcagcg gcaacacctt
      961 gctgctttcc gacagcagcc agacgcagcc agcagcccca cacagctggc ttggcgtacc
     1021 gctgcgctca caccgcggga ttatcggcgc actgctggtg caaagcagtg cttccacgcg
     1081 ctacagcgag cgcgatcagg agctgctgca attcgtctcg acccaggtgg ccacggccat
     1141 cgagcgtcag cagatgctca gccgcctgca gtttatggcg cagtacgatc agctcaccca
     1201 gctgcccaac cgcgaactgc tgcgcgatcg cttgcacaca gccctggccc gcgcgcgccg
     1261 cgagcagtcg caggtggcgc tgctgtttct cgatctggac aagttcaagc aggtcaacga
     1321 cagcctcggc catgctgctg gcgatcaact tctgcaaggc gtcgcccagc gtatccagct
     1381 ctgtctgcgt gaggccgata cggtggcgcg ctttgccggt gatgagttcg tcgtgctgct
     1441 ggaggacttc cattcggccg atcatgccag cgtggtggcc gagaaaatcc gccagagcct
     1501 caatcatccc ttcgagctgt gcgggcagag ccagaccatc ctgccgagca tcggcatcgc
     1561 cctgtatccg ctacacgccc aggatgaaca gcaactactg caacacgctg acaacgccat
     1621 gtaccgggcc aagcaaaaag gcggcaaccg cgcatacagc gccctcgaac tggaatgagc
     1681 gcgcataaaa aagcccgcca atcggcgggc ttctgattac tgcaggctta cttctcgacg
     1741 aaggcgcgct cgatcaggta atcacctggc tcgcgcatac gcggcgagat ctgcaggccg
     1801 aactcgttga gcacttcgct ggtttcatca agcatgctcg ggctaccgca gatcatagcg
     1861 cggtcgtcct gcgggttgat cggcggcaga ccgatgtcct cgaacagctt gccgctgcgc
     1921 atcaggtcag tcagacggcc ctgattttcg aacggctcgc gggtcactgt cgggtagtag
     1981 atcagcttgt ccttcagcgc atcgccgaag aattcattct gcggcaggtg ctcggtgatg
     2041 aattcgcggt aggccacctc gttcacatag cgcacgccgt gaaccagaat aaccttctcg
     2101 aagcgctcgt aggtttccgg gtcctgaatc acgctcataa acggcgccag gccagtaccg
     2161 gtgctgagca ggtacaggtg cttgccgggt ttgaggtcat ccagcaccag ggtgccggtg
     2221 ggcttcttgc tgataatcac ctcgtcacct tccttgaggt gctgcaactg cgaggtcagc
     2281 gggccatccg gcaccttgat gctgaagaac tccagatgct cttcccagtt cgggctggcg
     2341 atcgagtaag cgcgcatcag tgggcggccg ttgtcctgct gcaggccgat catcacaaac
     2401 tggccattct cgaagcgcag gcccggatca cgggtgcact tgaagctgaa cagcgtgtcg
     2461 ttccagtgat gaacgctgag aatgcgctcg gagttaaggt tgctcatgtg ccgggggctc
     2521 cgaagaatgg acgatgcctg cgcgcctgtg cgcaattgca cggcattgta atgagcggca
     2581 gaatatctgt taacttgatt atcaagatat aggttatcgg ttatatagat atgagattta
     2641 ccctcagaca actgcaagtc ttcgtcgccg tggcccagca tgaaagcgtg tcgcgcgcgg
     2701 ccgattccct ggccctgtcg cagtcggcca ccagtacttc gctcagcgaa ctggagcgac
     2761 aaaccgactg ccagctgttc gaccgcgccg gcaaacgcct gtgccttaac tctctcggct
     2821 tgcagttgtt gccagaagcg gtggcgttgc tcgaccaggc caaggaaatc gaacgcctgc
     2881 tgggcggcaa gagcggctat ggctcgctga atgtcggcgc caccctgacc gtgggcaact
     2941 acctggccac cctgttgatc ggcagtttta tgcagcgcca cccggaatgc cgggtcaagc
     3001 tgcaggtgca caacaccgcc tacgtggtgc agcagatcgc ccactacgag ctggacatgg
     3061 gcatgatcga aggcgactgc cagcacccgg atatagaggt gcagccctgg gttgaagatg
     3121 agttggtggt gttctgcgcg ccgcagcacg cgctggcgca gcgcggtgag gcgagcctgg
     3181 aagaactgac gcgcgaggcg tggattctgc gtgagcaagg ctccggcacg cgtctgacct
     3241 tcgaccaggc catgcgtcac caccccagca gcctaaatat ccgcctggag ctggaacaca
     3301 ccgaggcgat caaacgtgct gtggaatcag gcctggggat tggctgcatc tcccgcctgg
     3361 cgctgcgaga tgcgtttcgc cgtggcagcc tggtggcggt ggaaacccct gagctggatc
     3421 tgcgtcggca gttctacttt atctggcaca aacagaagta ccagaccgcc gccatgcgcg
     3481 aatttatcga actgtgccgc gcactaaccg ccggcgtgac gcgcagcgat cagatcgttc
     3541 tgccgactat cgcctgagaa cggcggagtt agccgagcag gataatcgcc cacaccagag
     3601 caatcaggct gagggcggtg aactgggcgg cgctgcccat gtccttggca ttcttcgaca
     3661 gcggatgcag ctccagcgag atgcggtcga tggccgcctc caccgccgag ttgagcagtt
     3721 cgacgatcag cgcaagtaga cacaccgcga ccatcagcgc gcgctcgacg cggctgacat
     3781 ccaggtaaaa cgccaacggc accagcacca ggttgatcag cagcagctga cgaaaggcgg
     3841 cttcaccggt aaaggcggcg cgcaggccag ccagggaata accggtggca ttgagaacac
     3901 gtttaaggcc ggtttggcct ttgaatggcg acatcattac aaccgtctcg agaaagcccg
     3961 gcaggctacg ccaagcccgg gagaaaagca cctcagctcg cgggaataga ttccagctgc
     4021 tgcaacagca acgccgcctg tgtgcgagtg cgcactccga gcttgcggaa aatcgccgtg
     4081 acatgggcct ttaccgtggc ctcggacacg ctcaactcat aagcaatctg cttgttcagc
     4141 aagccgtcgc agaccatggt caacacacga aactgctgcg gcgtcaggct ggccaggccg
     4201 gcactggcag ccttggcttc agccgacagg gcaatcgctt cctgagtctg ctgcggccac
     4261 cagacctcac catcaagcac cagacgcacg gcctgctgaa tggtttccag cgggctggat
     4321 ttgggaataa agccactggc accgaactcc cgcgaacgcg cgacgatgga cggttcttcc
     4381 tgagcggaga tcattaccac cggaatatgc gggtattgcc cgcgcagcag caccaggcca
     4441 gaaaagccgt aggcaccggg catattcagg tcgagcagaa ccagatccca atcagtcttc
     4501 tgcgccaggc aggcttccag ctcagcgatg ctggccgctt caaccagtcg cacatccggc
     4561 cctaacccca gggtcaacgc ctgctgcaaa gcactgcgaa acaaggggtg atcatcagca
     4621 ataaggattt caaaagcggc catggtgaat cctgtttttg ttatgtggat acccgcgagg
     4681 ggtactgtcc agggcacggt gaacagtgcg cacaggccgc ggggttgccg cgcaaccagg
     4741 cctgaaaacg cagaaaagcg accaagcatg cccagcactg acggggtggt caagcacgac
     4801 gctttgcggc acagttcgca gctttgccga cgagagcctg aaatgcaaag ccacgccctg
     4861 cgcgccgacc tgttgatgct gctgaccgca atgatctggg gctcggcgtt tgtcgcacag
     4921 cgcctgggca tggactccat cggccccttc ctctacagcg gcctgcgctt tgccttggcg
     4981 gcgctgattt tgctgccagt gctacgcctg ctggaaggtc gcagcagcag cgcgacagtg
     5041 gcaccactga accgccagct gctgcgtggc ggcgtgctga tgggcctggc actggccctg
     5101 gggatcaacc tgcagcaggt tggcctgctg tttaccagcg taaccaactc cggctttatc
     5161 accgggctgt acgtgatcat cgtgccgttg ctggggctgt ttatcggcca taagaccggc
     5221 ctgggcatct ggctgggggc ctgcctggcg gtggtcggca tgttcctgct cagcgttggc
     5281 gaaggcttca ccgtagcctc cggcgattgg ctgcaactgg ccggggcctt cgtctggggt
     5341 gtgcatgtgc tgctggtggg tttctttgct ggccgccatg acccgctgcg cctggccctg
     5401 gtgcaattca tcacctgtgc ggtgatcagc ctggtgctgg cggtgatctt cgaggaaatc
     5461 cagctgcaag cgatcatcgc cgccgggccg gcgattctct acggcggcat cttcggcgtg
     5521 gccataggct ttaccctgca ggtggtggcg cagaaagacg ccatcgcctc ccacgccgcc
     5581 atcattctct cgctggaagc ggtattcgcc gccatcgccg gggcctggct gctcggtgaa
     5641 tcgctggaac tgcgtggtta cttcggctgc gcgctgatgt tcgccggcat gctgctggcg
     5701 cagttgtggc caaagaaact ggcccactag ccgctaaaga aactcgcgca ggcggtcatg
     5761 cattggatca tccagcggca ccgctcgcgg cgccttggcg gccaggtagt gctgactgaa
     5821 gacatccaga taggcattca agccatcggc cgctcgctgg tcgccggcca gttccaggca
     5881 cagagcggcg acttcagcgg tgcagaaatg atcgtcgcgc ttggagcgcc gcagtttgta
     5941 gcgcgacagc tgctcggcct gcagactgag cacaggcagg ctgtcgaggt aagggctttt
     6001 gcgaaacatc ttgcgcgcct cgctccaggt ggcatccagc aacacaaaca gtggtcgctt
     6061 gcccggtgct ggcagcactt cgcagaccac ccgctcagcg gcgacatatt cgcccggaaa
     6121 caccagataa ggctgccact gcggatcgcg cagcaacgcc ggcaacccgg catccacttc
     6181 aacccgcgac cagccaaacg ccgaggtatc ggccaacacg tcggcaatca gccagccggt
     6241 attgcttggc ttgagcgcct cgacatcgtg catcagcaga cacaccgctg aggttgcggc
     6301 aatacgcggg cgccaggcgc acaggcagtg ggtcatcacc aggcggcaat catcacaacg
     6361 caccgagcgg ctaccacggg caagaaaagg tttggcactg cgggcaatcc gcgcagcccg
     6421 caaacgcgcg acagcgtggc tcattgccgc cctcggccaa aggtcgcaac agataggcct
     6481 atgggggtgc agctaaacag accggcagaa aagcaaaaca tgcagcgccc ctggcacata
     6541 acggagggcg cgcagtctag caggacagaa catctcatag ccctgctata cgacggcctg
     6601 ctaccgcggc ggttaaaagc gatgattcag cgccaaaccg atgaaatgaa tgtgcgtttc
     6661 atactcgcca ctgtagttac cgccattgcc agtgcagcgc acgccactgt cctggtatag
     6721 ccctccactg cccggcaccc accctgctgg agagcagtta tcggtgtaat ccatcttgcc
     6781 ggtggcgaac ttgacgaagc tgtaggcaag gtccactgat gtgtcctggg tcacgcggta
     6841 attggcccca agcgataacc agtagcgatc ggcatcgggg aatgccgggt ggcgcaactg
     6901 agtcgaactc accggtgatt tgtcgtaggc cacacccgtg cgcagtaaca gcttatcgtc
     6961 gtactgatag ttcatcccca gggacaattt ataggtgtcc ttccagtcct gcttgactgc
     7021 cagatcaccc tcggtcacac cgttgaagta gggatagccc gaaacctgat caagaccaat
     7081 gcggacttca tcgagacgcg aatggcgagt gaaggttagg tcggccatta gcgcaatctg
     7141 ctcattaagc tgatgaaagg cattgaagga cagcatctcc ggcgtatcga tggcggtctt
     7201 ggccttgctg ttggggtgca agcgctcccc ggcaaaatcc cccggattga cccagttgcc
     7261 ttcatcaaat agctgctgca atgcctcggt gggcggtaca tagatatcaa ctatcggaat
     7321 ggtgaatccg ccatccagcg aggtgtccgg ggatggcacg gcgccctgca cgctggcaaa
     7381 actccatttg ctctcgccgt ccagcgtgtg acggatattc gaccggtagg ccacgccgaa
     7441 acgcgtcgat tcagtgggct cccacaaata accgatgttc cagccgaatc cccagtcatc
     7501 acccttcacg cggaaataac catcgccctg gacgttatcc gcaaagttat tagcgacaca
     7561 gtcgacgaaa ccatcccgat cagccacgcc cctgcaggag gtgatttcat ctggcacggg
     7621 caccccggcg atcaggccta caatcggacc cagaatcggg tcggcggcat tgcgcgtact
     7681 gtcgtaatta gcatcgacga attgcccggc caactgccgc gatgcccctt gcacatctgc
     7741 cgccccacgc tgtatcgatt tgatgtactg ggcggagacg ccaaaaccga tactgtggtg
     7801 ttcattaaaa cgaatggcga tgctcgggtt gaagttcact gtttccaggc tcgccgattg
     7861 gatgccatag cgtccggccc agtcctcctt gtaatcgagt ttggcaccaa agggcgtaaa
     7921 gatgcccagg ccgacactga gccagtaatt gacctcatgt gaaaagtaaa gattcggcgc
     7981 ggctgcggcc tcaggaagaa atgagcctgc gtcgccactc accggctgac cgaatacatc
     8041 ggcactgccc gtatcctggt attttccatc cggcagcaga atgcttatac ccgacgttac
     8101 ctgagtaccc ttgagccgag ccaggccagc cgggttgtag aatatggtgg acgcgtcagc
     8161 tgcctcggcg ccattggaat gggcggaacc ctgagcagaa acagattgcg aaccgaagtg
     8221 gtacccagat gcctgtacca ttccataggg ggtcgtcagt ggagctacgg caaaaaacac
     8281 caatgaccca tagcgtgtaa ttcttgtcat tattggatgc actcctgcgc aagacggaaa
     8341 cacccagggg ctgctcatac atcgcctgga aaggccagag ctaagagcag tcgcacctga
     8401 atcgctggaa tcgctttacc agcctatttt ctccgactca gaaacgtacg cgacctatcc
     8461 cacgtctttg gtcgtgggat gagactgaag gcaggcaaag gtgtcgcgat cggtgtcgcg
     8521 atcggtaaca acttggagaa actgtcagcc tcgcgaacgc ggcgacactc tgcacacgaa
     8581 catccgccta acgagcgggt ctaatcgcat taactgaact ttaggaagtt accccatgct
     8641 gcgtttcatc gttccagccc tcgccttcgg cctactctta ccgagcgccc atgcagcctc
     8701 gttaaaggat tacgaactga ccaaaacgct ggaaaaagtg gcgcgcgaaa gcagtgtggg
     8761 tacaccgcgg gcgatcaacg aagacattct cgatcaaggc tacaccgttg acggcagtga
     8821 gctggtcaac cacctcagcg tacgcagcga acatgcggcg cagatgcgcg gcaacccgga
     8881 tgtagtgcgc gcgcaactgg ccaacagcgt gtgccgcaat tccggttatc gccagttgtt
     8941 gctgcgcggc gctgcgctgc gctatgaatt cagcgaatac aaaagcaacc gcccggtgac
     9001 caccgagcgt ttcagcaaag cagattgcgg cctgtaaggc tcagccgcca ccctgttgcg
     9061 cgcgtcgctg ctcatcatcg gcgcgcaaat ccgcgagcaa cgcgtgcatg taacgcgaac
     9121 gccgggccgc accttcaagc cgctgcaaac actcttcttc caggcttatc tggttgttgc
     9181 gcgcggcctg ctgcaacagg cggtacaaat ccacatcaag atccagggtc aacttaggca
     9241 tacctgcccc tcctttcccg cgacacactg cgcctgctaa cgcagacagc gtggttctcc
     9301 ccgcccttgc acggcggctt gctcctcggc actgagcaac tcgccaagcg gtttttccag
     9361 caactcggca gcggcctcca gcacatgcgc ccagctcgcc tggttggcga acagcatgcc
     9421 ggcttcatcc agggtgccac ctcgattacg gtagcccagc acacgactgt gtcgagcgtc
     9481 acccagcagc ggccacagat gcccacgggc gacttccggg cgcatatggg tgagcagcac
     9541 gcgctgtacg ctggcagccg ggaataaccg ctcaacccgc tcggcatcga caatcaccgc
     9601 ctgttcccag gtatcgcgag ccgcacgaaa acgccccggc tcttgcaagt aaaccagacg
     9661 ccaggcgcat ccggccgcgc tcagacgaac ggccgcgcgc tgcatttctg ccaactgata
     9721 actgccggta gcgatcagca acaaaggctg ctcgccttgc tgctcagcca gcacaacggc
     9781 gccatcctgc gccagctgct cggcctgttc gcgggtgaag acgcaaggcc gctcgcgctt
     9841 ggctattacc atgcaggcga tctggccgcg cgcctggtag atgccgggta gcagcgccaa
     9901 taccgaattg tgatcggctg gaaacagcac ccgcatcata tcgctcatct caccgagcaa
     9961 ggcctcgcag aaggtggtgt cctggtgtga ctgctgattc ttgccgttct cccaggtgtg
    10021 cgaggtggcc accagcggcc aaccgagcca gcctgccggg cgcccggcct ctttctgctg
    10081 acgggcaaag atgatccgct ggcgcaccgc gccgagcatc ttcacgcaga aggcctcgta
    10141 gctggccacc agattcaggc cgccctggtt ggccaggcag gcggacacca cggcctcctc
    10201 attgagcgcg gtgatgattg cgccatccaa cgcttccaac tcactttccg gcgcagtcac
    10261 ccggtgcttt agcgccttga gcacgccgcc caggcgattg ctggccagct catcggggtt
    10321 gccgatacgc gggcgcaaca gcgggttggc ctgcaccaga tcgacaaaga agcgatccag
    10381 ggccagcatt ggcgaacagg cgctgtcctg atactgcagc tcgggcagtt ttggcagcgc
    10441 tggatgacgc tgcgccaacg ggttatcgcg ctccagcgtg cgggaacctc gattcgcaaa
    10501 cagagcgcag gcgccactca gctcatccgg agcgacccag agctgcgccg catgctgatt
    10561 gaacagctcg cgggcagcct catcggtatg cggattagct ggtaacggca agttgtgcgc
    10621 cgcattgctg cccgcgccgt agaaaccgaa acccttgagc gtttcggcaa tgccgtaggg
    10681 catgggcagc ggatagttca gcacgccatc gcgcagctcc tgcacgcggc gactcatgcg
    10741 ctgctccata tcccacaagg cgcagacaaa ggcagccggg tcgcggccat cgaaactcac
    10801 cggatcaaag ccgcaatgac gtaggtgctc acgaaagtta tccagcccgg cgggcgtcgc
    10861 caactcggtg cgctgctcga tacgccggcc gttggcaatc atcagcggca acgccacacc
    10921 gcagtcctcg gcgcgccacc agcgcggcat ccagtcactg ccgcgttgct cctcggccgc
    10981 accatcggag aggaaggcca ccagcttctc gccgggtaac ggcagatggg catattgcag
    11041 ctcggcaaag cccagataac caccttcggc aatgccgcca gccgtgtgcg gattgacgtg
    11101 gctgcccagc ggcgcgctga cctgaccggc cgcgttctgc acatagctgt agaagtccgc
    11161 caccaactgg ctcatcccgg cttcgtcaca ggggtaacgc tcggcctgct ccggatgctg
    11221 attggccgtc agcaggttga gcgcctcgat ggccgccacg cagtgaccct gtcccatcaa
    11281 ccagccacgg gtttccccgg tcaggttatt cagcgccaga taaccggcat aggccggcac
    11341 catattcagc gcgccgccgg tatgcccttc gggcgagact ttgaagtcct ctgcggccaa
    11401 cgccacacca tcgaggcgca cccgccgggc ataggtcata tgcaccacca gccacagccc
    11461 cgcggccgtc agccgatcca gcgcacgaaa gtgccggtag acgctcggca gatccgcctg
    11521 cgcgcccgcc tgcaccagct ggtgggccat gcggtaaacc gcggcacagg tctgcggact
    11581 atgttgcaaa gggccataac cacgctgcca ggcggcgaag gtcggctcaa ggcgggcatg
    11641 ctcggccaac tcgctggcgc tgggaaatag ctgggtcata ggcgtctccg atagttttag
    11701 tcagcttaga gcgcgggata caggcagatg ctgacctgta tcaaaaccct cggttgcttg
    11761 ataggacagc cttaagcgca acctattcaa ctcgatcaag gattagccat ggccctgctc
    11821 aatttcctcg gcgcaattca acaagtcacc ggttcctgct acttgatcga aagccgtgac
    11881 ggcgcacggg tattgctcga ctgcggcatg cgccagggcc gccgcgaaga ggaagacggc
    11941 aaccgcgcgc cgtttgcctt tgacccgcac agcctggacg ccgtggtgat ttctcatgca
    12001 cacattgacc acaccggcct gctgccaaaa ctcacggcag caggctatcg cgggccgatc
    12061 tttgccaccg acgcgacctg tgagctgatg gagttgatgc tgctcgacgc cgcgtttctc
    12121 caagagaaag acgccgagtg ggaaaacaaa tggcgcgcgc ggatgggcaa ggtgccaatc
    12181 agcccgctgt ataccaccgc cgatgccgaa caggcactga gccaacgccg accgatgatt
    12241 tatggcgaaa gccgcgaagt ggccaagggc gtgcaggtca cctttcacga cgccgggcat
    12301 atcctcggct cggcgattgt cgaactcgag gtacaggacc atcacctgac ccgccgcctg
    12361 gtgttctccg gcgacctggg caacacctgc tcaccgctga tgcatgaccc ggccatcctc
    12421 aagcgcgccg acgtggtgct gctcgaatct acctacggcg accgcgacca ccgctgcagc
    12481 agcgataccc tcgacgaact ggcggagatt ctgcagcagg cccatcgcga cggcggcaac
    12541 gtgctgatcc cctccttcgc cgtcggccgc acccaggacc tgatctacta cctcgggcgt
    12601 ttctatcaag aaggccgcct gccgcagcag gcggtgtttc tcgacagccc catggcgatc
    12661 cgggccaacg ccatctactc gcgctttcag aaccagtttg ctgccgaaga ccgcgcggcg
    12721 ctggcggcca agaaggtcaa acgtatcgaa gactggctac ccatcctgcg ctgcacaccc
    12781 agcccggaag agtcgatggc aatcaaccga ataaagagtg gtgcaatcat catcgccggc
    12841 agcggtatgt gcaccggcgg gcgcattgtc catcacttca agcacaacct
//
LOCUS       DASRUM010000286         3472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1978223, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000286 DASRUM010000000
VERSION     DASRUM010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978223"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2340)
                     /gene="pheT"
                     /locus_tag="VFF22_19015"
     CDS             complement(<1..2340)
                     /gene="pheT"
                     /locus_tag="VFF22_19015"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZX18945.1"
                     /translation="MKFSEQWLRSWVNPQVSRDDLVARLSMAGLEVDSVSLAAGVFSG
                     VVVGEVLSTEQHPDADKLRVCQVSSGAETFQVVCGAPNVRPGLKIPFAMIGAELPGDF
                     KIKKAKLRGVESNGMLCSASELQISEDNDGLMELPADAPVGQDIRVYLDLDDASIEVD
                     LTPNRGDCLSLAGLAREVGALYAAPVTRPVVAAVTPSHDEVRPVEILAAQACPRYLGR
                     VVRNVDLSKPTPLWMVERLRRADVRSIDAAVDITNYVMLELGQPLHAFDLAEINGGIR
                     VRMAEEGEKLVLLDGQEVSLRSDTLVIADHSRALAIAGVMGGEHSGVNEKTRDLFLES
                     AFFDTIAIAGKARSYGLHTDASHRFERGVDWQLAREAMERATALLLEVVGGEAGPIIE
                     VVNQEHLPSVAPVTLRAERIEQMLGLKMDDVEIVRLLAALGLDIRADGEGQWQVGVPS
                     HRFDISLEVDLIEELARLYGYNRLPVRYPQARLAPQAKAEAAVELPALRRLLVARGYQ
                     EAITYSFIDPKLFELFNPGVAPLMLANPISADMAAMRSSLWPGLIKALEHNLNRQQSR
                     VRLFESGLRFVGQLEGLKQEPMLAGVICGGRLSEGWAHGRDDVDFYDVKADVEALLAS
                     AGAGDSFAFVPGEHAALHPGQTARIEREGRVVGFLGAIHPELSKALGLDQAVFMFELV
                     LGEITQGVMPKFQELSKFPEVRRDLALLVDRDVPAQDLLGAIREAAGEWLTDLKLFDV
                     YHGKGIHPHRKSLAVGLTWQHPLRTLNDNEVSTTTQNILT"
     gene            complement(2386..3402)
                     /gene="pheS"
                     /locus_tag="VFF22_19020"
     CDS             complement(2386..3402)
                     /gene="pheS"
                     /locus_tag="VFF22_19020"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HZX18946.1"
                     /translation="MENLDVLVSQALEAVSHTDDVNALEQLRVHYLGKKGELTQVMKT
                     LGNLSAEERPQAGALINAAKDQVQDALNSRKATLEQAALSAKLAAEKIDVTLPGRGQV
                     SGGLHPVTRTLERVEQFFTRIGYGIAEGPEVENDYHNFEALNIPGHHPARAMHDTFYF
                     NANMLLRTHTSPVQVRTMESQQPPIRIVCPGRVYRCDSDITHSPMFHQVEGLLVDEGV
                     SFADLKGTIEEFLRVFFEKPLGVRFRPSFFPFTEPSAEVDMQCVMCSGKGCRVCKQTG
                     WLEVMGCGMVHPNVLRMSGIDPEKYSGFAFGMGVERLAMLRYGVNDLRLFFDNDLRFL
                     AQFR"
BASE COUNT          804 a         1088 c          925 g          655 t
ORIGIN      
        1 ggtgagaata ttttgcgttg ttgtactcac ctcattgtca ttaagagtgc gcaatggatg
       61 ctgccaggtc aagccaacgg caaggctttt tctatgagga tgaatacctt taccgtgata
      121 cacgtcaaat agcttgaggt cggtcagcca ctcgcctgct gcttcacgaa tcgcaccgag
      181 caaatcctgc gcaggcacgt cccgatcgac cagcaaagcc aggtcacgcc gtacttctgg
      241 gaacttcgac agctcctgga acttcggcat aacaccctga gtgatctcac ccagcaccag
      301 ctcaaacata aagacagcct gatccagacc cagcgccttc gacagctcgg gatgaatggc
      361 accgaggaaa ccgactacac gcccttcacg ctcaatgcgc gccgtttgcc ccggatgcag
      421 cgcggcatgt tcgcctggca caaaggcaaa gctgtcgccc gcacccgcac tggccagcaa
      481 cgcctcgaca tcagctttca catcatagaa atcgacatca tcgcgaccgt gcgcccaacc
      541 ttcagacaag cgaccaccac aaataacacc cgccagcatc ggctcttgtt tcaagccttc
      601 gagctgacca acaaagcgca agccgctttc gaacagacgc acacgcgact gctgacgatt
      661 caagttgtgc tcgagcgcct tgatcagacc cggccacaaa gaagaacgca tcgccgccat
      721 gtctgcagaa atcgggttag ccaacataag cggcgcaacg cctggattga acagctcgaa
      781 cagcttggga tcgataaagc tgtaggtgat cgcctcctga taaccacggg caaccagcaa
      841 acggcgtagc gcaggcagtt cgacagcggc ttcagccttg gcttgcggag ccagacgcgc
      901 ttgcggatag cgaaccggca agcggttata gccgtacaaa cgagctagct cttcgatcag
      961 gtcgacttcc agactgatat caaagcggtg acttggcaca ccaacctgcc actgcccttc
     1021 gccatcggca cgaatgtcca agcccaaagc agccagcaaa cggacaattt ccacatcatc
     1081 catcttcagg ccgagcatct gctcaatgcg ctcagcacgc agcgtgaccg gcgcaacgct
     1141 cggcaaatgc tcctgattga cgacttcgat aatcggcccg gcttcgccac cgacgacttc
     1201 cagcaaaagc gcagtcgccc gctccatcgc ctcgcgggcc agctgccaat ccacgccacg
     1261 ctcgaagcgg tgcgacgcat ccgtgtgcaa accgtaagag cgagccttgc cagcgatggc
     1321 gatggtgtcg aagaacgcac tttcaaggaa caggtcgcgg gtcttttcgt tgacaccgct
     1381 gtgctcgccg cccatcacgc cagcaatcgc caaggcacgg ctgtgatcgg cgatcaccag
     1441 cgtatcgcta cgcaggctga cttcctgacc atcaagcagg acaagcttct cgccctcctc
     1501 ggccatacgc acacggatgc cgccattgat ctcggcaaga tcaaacgcat gcagtggctg
     1561 acccagttca agcatcacgt agttggtgat gtcgacagct gcatcgatac tgcgcacatc
     1621 ggcgcgacgc aggcgctcaa ccatccacaa cggcgtgggc ttggacaaat cgacattgcg
     1681 cacaactcga ccgaggtaac gcgggcaggc ttgcgcagcc agaatttcaa ctgggcgcac
     1741 ttcatcatga ctcggcgtaa ctgccgcaac taccgggcga gtgacaggcg cagcgtacaa
     1801 agcaccgact tcacgcgcca ggccagccag cgacaggcaa tcaccacgat tcggggtcag
     1861 atcaacttcg atgctggcat catccagatc cagatagaca cgaatgtcct ggcctaccgg
     1921 cgcatcagcc ggcagctcca tcagaccgtc attgtcttcg ctgatctgca gctcagaggc
     1981 cgaacagagc atgccgttgg actcaacgcc gcgcagcttg gctttcttga ttttgaagtc
     2041 gccgggcagt tcggcgccga tcatcgcgaa cggaattttc aggccaggac gcacattggg
     2101 cgcgccacac accacctgaa aggtttccgc gccgctgctg acctggcaca cacgcagctt
     2161 atcggcatcc ggatgctgtt cggtgctcag cacctcgccc accaccacac cactgaatac
     2221 accggcggcc agcgatacgc tgtcgacttc tagaccggcc atggacaggc gggcaaccag
     2281 atcgtcgcgg gaaacctgcg ggtttaccca gctacgcagc cattgttcac tgaatttcat
     2341 actgtctgtt ctcctgaacc aattcgatta cggaatgcga gggcgctagc gaaattgcgc
     2401 caggaaccgc aggtcgttat cgaagaacag ccgtaaatcg ttgacgccgt aacgcagcat
     2461 ggccagacgc tcaacaccca taccgaaggc gaagccggag tatttttccg gatctatgcc
     2521 cgacatgcgc agcacattcg ggtgcaccat gccgcagccc atgacttcca gccagccggt
     2581 ctgcttgcac acgcgacagc ctttgccgct gcacatcacg cactgcatgt cgacttcggc
     2641 cgacggctcg gtgaagggga agaaagacgg acggaaacgc acgcccaggg gcttctcgaa
     2701 gaacacccgg agaaactcct cgatggtgcc cttgaggtca gcaaaactca cgccctcgtc
     2761 gaccagcagg ccttcgacct ggtggaacat cggcgaatgg gtaatatcgg aatcgcagcg
     2821 gtacacgcgg ccggggcaaa cgatgcggat cggcggttgc tgcgattcca tggtgcgcac
     2881 ctgaaccgga gaggtgtggg tgcgcagcaa catgttcgcg ttgaaataaa aggtgtcatg
     2941 catcgcccga gccgggtgat ggcctgggat attgagtgct tcaaagttgt gatagtcgtt
     3001 ttccacttcc gggccttcgg caatgccgta gccaatacgg gtgaaaaact gctcaacgcg
     3061 ctccagtgtc cgggttaccg gatgtaagcc accagaaacc tgaccgcggc ctggcagggt
     3121 cacgtcgatt ttttcggctg cgagcttggc gctcagcgcg gcctgttcca gagtggcctt
     3181 acggctgttc agagcatcct gaacctgatc cttggcggca ttgatcaacg caccggcttg
     3241 cgggcgttct tctgccgaaa gattgcccag ggtcttcatc acctgagtca attcgccttt
     3301 cttgcccaga tagtgaaccc gcagctgctc cagggcattc acatcgtcgg tgtggctaac
     3361 ggcctcaagc gcttgcgaga ccagcacatc cagattttcc atttacagac tccagatacg
     3421 aaatagggga agagcttgaa ggctcttccc ctattggtga cgtttagcac cg
//
LOCUS       DASRUM010000287        54582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2314985, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000287 DASRUM010000000
VERSION     DASRUM010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 54582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54582
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2314985"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..881)
                     /locus_tag="VFF22_19025"
     CDS             complement(63..881)
                     /locus_tag="VFF22_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009618757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HZX18947.1"
                     /translation="MSHAIEAYHPGVFDLTHKLTVEKHGNTALITINQPPANTWDRDS
                     LIGLKLLIEHLNRDDDIYALVITGQGGKFFSAGADLKLFADGDKARAREMARRFGEAF
                     EALRDFRGVSIAAINGYAMGGGLECALACDIRIAERQAQMALPEATVGLLPCAGGTQH
                     LSWLIGEGWAKRMILCGERVDAETALRIGLVEQVVDSGESRGHALLLAAKVARQSPVA
                     VRTIKPLIQGARERSPNTWLSEERERFVDLFDADDTREGVNAFLEKRDPQWRNK"
     gene            complement(1105..2271)
                     /locus_tag="VFF22_19030"
     CDS             complement(1105..2271)
                     /locus_tag="VFF22_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isobutyryl-CoA dehydrogenase"
                     /protein_id="HZX18948.1"
                     /translation="MDFELSDEQRLLVDSARQFASRELAPHAADWDRDHHFPVDVIKR
                     AAEQGYLALYIKEDDGGLGLSRLSASLIFEQLSAGCIATTAFLTIHNMASWMLASFAD
                     QALKDAWLPRLVSGELLASYCLTEPDAGSDAAHLRTRAKRDGDDYVLDGSKCFISGAG
                     STDVLIVMARTGEDSGAKGVSCFLVPADAEGVKYGRNELKMGWRAQPTRTITFEGVRI
                     PAGNRIGPEGQGFVYAMKGLDGGRINIASCSLGAAQAALEQSLRYVEERKQFGKPLSD
                     FQALQFKLADMLTDLTASRQMVRLAAHKLDHGHSEASLYCAMAKRFATDHCFTLCNEA
                     LQLHGGYGYLNDYPLERWVRDSRVHQILEGTNEIMRVIIARRLLDQGGMLDRLL"
     gene            complement(2278..3525)
                     /locus_tag="VFF22_19035"
     CDS             complement(2278..3525)
                     /locus_tag="VFF22_19035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX18949.1"
                     /translation="MQRLTTALALWAGLTACAQAAEPITLGLNYPRTGPYKEEGLAQM
                     RGALLAIDELNAQGGVLGRPLRLSSKDTASRPAKAVKNVDKLASEGAAMLFGGSSSAV
                     AIASGKRAAQHGLLYFGTLTYSNDTTGKDGHRYMFRECNNAWMSARVLGQYLSKNLPN
                     KRYFYITSDYTWGHTTESSLRQTTNSEDSGQHPGLKVTFPGARLADYQDALTQAAASN
                     AEVLALVLFGEDLVRAMRIAKDLGLTERMQIVAPNLTQSMVEQGGPGLMEGVIGTEPW
                     TWRVPALEKSVRGEAFVKDFSQRYEMYPSSSAASAYSIVYQWADAAKRANSLDSEALI
                     KALEGHSYQLLKNQQTWRALDHQNLQTVYAVKVKERAEVLKDPLKQDYFEIVDRLDAT
                     QAAPSLAEWQAERRAAGQPLTLQ"
     gene            complement(3627..5135)
                     /locus_tag="VFF22_19040"
     CDS             complement(3627..5135)
                     /locus_tag="VFF22_19040"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZX18950.1"
                     /translation="MAKAIAQLIDGEWRESRAREFIEVTDPATQEVLALAPKATAEEI
                     EAAIASAKAAFLTWREVPVSDRARVMLRYQHLLKEHHDELAEILAGETGKTLADAKGD
                     VWRGIEVVEQACNIASLMMGETMENVARDIDTASWIQPLGVCVGVTPFNFPAMIPLWM
                     FPLAIAAGNTFILKPSEQDPLTPNRLAELFIEAGAPKGVLQVLHGAREVVDSLLTHPD
                     IRAISFVGSVPVGQHIYRTGTAHLKRVQAFAGAKNHMVIMPDANKQQVLSNLVGASCG
                     AAGQRCMAISVAVFVGESKQWIDELQAQMADLQPGYWTDGHAAFGPLISQQAKQRVLR
                     LIAEGKAEGAECLLDGSHCAVEGYPNGNWIGATLFRGVTTKMSLYREEIFGPVLVCME
                     VDTLEEAIELVNASPYGNGTSIFTRSGGAARHYQHAVEVGQVGINVPVPVPLPFFSFT
                     GWKGSFYGDLHAYGKQGVRFFTETKTVTSRWFDEDSVAAGSPAGPNMTIQLK"
     gene            5318..6199
                     /locus_tag="VFF22_19045"
     CDS             5318..6199
                     /locus_tag="VFF22_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009315247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZX18951.1"
                     /translation="MRAFVDTSNWPLPANGVRFITPPRLRRLLAKHPLGQGCYPLALG
                     YYPEAQGHRMHRPQPEDHLLIYCRAGQGWLETADGRLAVSGGDLLLLPKGVMHAYGAD
                     VDKPWTLYWVHFDGELVADFLKPLGKGPMWRIGVQPRLLAEFDALLNLRKQGLNLAHF
                     VHAAHQLQVLLTSLAVLPARTTLKSGRVLDVDAVQAVMRAHLHDSLNLDELAAQFKLS
                     RFHFAKTYRALTGHAPIQDFIQLKMAHACRLLDEGEQGIRQVAEQLGYDDVYYFSRLF
                     RKVVGMAPSHYRALHQG"
     gene            6267..7301
                     /locus_tag="VFF22_19050"
     CDS             6267..7301
                     /locus_tag="VFF22_19050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18952.1"
                     /translation="MSTKRGVIQMDTVRDIEERMTSFLQSQDHVTLLDGHVPQPDGVK
                     IADYLFFNRQAVVELKTLKIDPKDKILDGAKSLMESDGFPLIFGDYDLETAMKNTPGG
                     QEHLDKIFSRATRMVEGVLRNAKKQIASSKRLLNLDPDTPGVALILNDTVESIPASRL
                     VDRFSSWLTGDGKDPGRFSEIDFIILIQTTYRLQQGSGRRTYLPTFIISNPFNAHRHH
                     KIEQEIQLFLQAWARSQGHNFESTSETSGLKFEHNHGPRPGPRSLQELVEAQYRARRY
                     MSEWSEERLIAHGKDVIQKMLLIFLIDAEKPSEADSHRYIKQFTELLEEGRIRGFDLR
                     KVLKEIPRTR"
     gene            complement(7305..7474)
                     /locus_tag="VFF22_19055"
                     /pseudo
     CDS             complement(7305..7474)
                     /locus_tag="VFF22_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019750380.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine recombinase"
     gene            7606..8298
                     /gene="ung"
                     /locus_tag="VFF22_19060"
     CDS             7606..8298
                     /gene="ung"
                     /locus_tag="VFF22_19060"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003293600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZX18953.1"
                     /translation="MSAADNVKLEAGWKAALHEEFEKPYMRELGDFLRSEKAAGKQIY
                     PPGSLIFNALNSTPLAQTKVVVIGQDPYHGPGQAHGLCFSVQPGVKTPPSLVNIYKEL
                     KRDLNIDIPDHGNLQYWAEQGVLLLNTSLTVEHGIAGSHAKIGWQTFTDKVIEVASQQ
                     QEHLVFLLWGAHAQSKEKLIDSSKHLILKSVHPSPLSAHRGFIGNGHFSRTNKFLQQH
                     GLSPIDWRLPEA"
     gene            complement(8306..9175)
                     /locus_tag="VFF22_19065"
     CDS             complement(8306..9175)
                     /locus_tag="VFF22_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HZX18954.1"
                     /translation="MSVLRWPAVKTLLLLLCLSFLISACSRVGLAYRNLDWLVPWRLN
                     DYLNLNSQQQAWLKPRLQTHLQWHCSAELPRYIDWLQTTESILAQPQPDSAQLLEQFA
                     QFDAALKRISVEITPTAIELLQGLSEQQVNELYAAIDEDNLEDRQDFLDPPLATQISE
                     RQTRMQERLRSWLGRLNNAQIEHIAAWANGLGEQNRLWLENRQLWQAELRKVVAERDS
                     ADFAERLTRLLQQRESFYNDEYRANYGRSRQALATLFSQLLSSSDEAQRERLSHRLRD
                     LRRDLAEQQCDAA"
     gene            complement(9323..10363)
                     /locus_tag="VFF22_19070"
     CDS             complement(9323..10363)
                     /locus_tag="VFF22_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HZX18955.1"
                     /translation="MPPMPKLFALTGWQSWWATPLVGLLGGYLASLIGWPLPWIIGSL
                     LAVIAARCSGWLITELPGGRKTGQWLVASAIGLHFTSEVLSQLLSHFGVILLGALLTL
                     LLSLIGIIVLRRAGLDRATAFFASMPGGASEMVNLAQRHNAETAKVAAAHSLRLLLVV
                     LLIPAIFTWSLPPIEPPAPAAVSWLWLAVLLPGGALLALLWGRLGQPNPWMLGPLTVC
                     AVASVSFDLHLGLPDGLGQVGQWLIGCALGCHFDRNFFRSAPGFLARILLFCLLAMLA
                     AAALGQGLGWLAGENQNALMLGMMPGGITELCLTAEALHLSVALVTALQVLRLFLVMF
                     LAEPLFRLWQRS"
     gene            complement(10347..11864)
                     /locus_tag="VFF22_19075"
     CDS             complement(10347..11864)
                     /locus_tag="VFF22_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HZX18956.1"
                     /translation="METLNFLMQGFEVATRPENLLVALFGAFVGTIVGLLPGLGPING
                     VALLLPLAFALGLSPETALILLAAVYLGCEYGGRISAILLNVPGDAAAVMTTLDGYPL
                     ARQGKAGIALSLSAVSSFIGSMIATCGVVLFAPLLAKWAVAFGPAEYFVLMIFAIACL
                     GGMVGDKPVKTLMAALIGLALATVGVDSTTGVYRFTFGSVSLSDGIQFVIVVIGFFSV
                     SEVLLMLEKTHNGQKAVKASGRLLFNFKEFCFTFWTMVRSAVAGFVIGVLPGAGATIA
                     SAMTYMTEKRMAGPNGKFGDGDLRGLAAPEAANNASACGSLIPMLTLGVPGSGTTAVM
                     IGALTLYNITPGPLLFEQQPDVVWGLIASLFIGNVILLVMNIPLVGLFARMLSVPNWV
                     LVPAITVISMVGVYAVHSTTFDLVLMIGLGVFGYILRKLDFPLSALILGFVLGELMED
                     NLRRALSISAGDLSILWNSPISLCLWALTALMLALPGLRWWRSRRAAQLANAANA"
     gene            complement(11867..12310)
                     /locus_tag="VFF22_19080"
     CDS             complement(11867..12310)
                     /locus_tag="VFF22_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HZX18957.1"
                     /translation="MYVRLFAASWLLFCAVLAVIAWGFQAPFAYDPVGPRAYPLLLLS
                     LMAAGSLWLVFKPGLLEQTLNTRAAVRAVLCVLALLAYALLFEVLGFVISTTLATFAL
                     GLLFTGRPLPCAISAVLMGVLLYGLFDYLLDVPLPLGLFEAFVES"
     gene            complement(12452..13429)
                     /locus_tag="VFF22_19085"
     CDS             complement(12452..13429)
                     /locus_tag="VFF22_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003303253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZX18958.1"
                     /translation="MKTAFTRIALATACMAFAGQLLAAEPKRPECIAPAKPGGGFDLT
                     CKLAQSGLKDSGLLKAPMRVTYMPGGVGAVAYNAVIAQRADDPGTITAFSSGSLLNLA
                     QGKFGRYDETGVRWLAAVGTDYGAITVRADSPYQSLDDLVQALKKDPTSIVFGAGATI
                     GGQDWMQTALIARLAGIDPKNLRYVAFEGGGETLTAMLGGHVQVTSSGLGEVTPQLAA
                     KKVRILAVLSDERLPGKLADIPTAKEQGYDIVWPVIRGFYMGPEVTDEQYNWWKQQFD
                     TLLAREDFATLREQRDLLPLSVTGDELKALVFKQVEEYKALAGEFGLMQ"
     gene            complement(13459..14730)
                     /locus_tag="VFF22_19090"
     CDS             complement(13459..14730)
                     /locus_tag="VFF22_19090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015802 - basic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OprD family porin"
                     /protein_id="HZX18959.1"
                     /translation="MPTASHQALSPVHLLSLAISTALLAPAAHADFVADSKASLTTTN
                     IYLNRDFREGTSSQQNKRDEWGQGFWLDVQSGYTEGTVGFGLDALGVVGVKLDSGGGS
                     TGTDLLPVQDDGGTPDQFGRLGLTAKVKVSNTELRYGSHIPELPVVKASDSRLLPQVF
                     EGGLLTSSEIDGLTFTGGRLDKVIDRASTNSEELILNSKNRRFAAGITADHLDLMGFD
                     YSFNKQLKGRYYFADLDDIYRQHFFGLLASQPLGSGTLSADVRMMLSDDSGASNAGKI
                     DNRAFNAMLSYAIDGHKLGLGFQDMSGDTGYAYIDGSDPFLVNFVQINDFANADERSW
                     QARYDYDFGKLGVPGLSFMTRYIKGSDAQIAGTDDTGGEWERDIEVKYVVQSGPLKDV
                     YVRLRNASFKSDFARDADENRVIVGYTLPIW"
     gene            14937..15608
                     /locus_tag="VFF22_19095"
     CDS             14937..15608
                     /locus_tag="VFF22_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX18960.1"
                     /translation="MRILLVEDHLPLAESVAQAMRGTGLTVDMLHDGVAADLALSSED
                     YALAILDIGLPRLDGFQVLARLRERGNTLPVLMLTARGEVKDRVHGLNLGADDYLAKP
                     FELSELEARVKALLRRTVLGGERQQRCGNLLYDLDTRRFSLADALLALTSREQAVLEA
                     MIARPGRVMSKEQLAAQVFGLDEEASSDAIEIYVHRLRKKLEGGGVRIVTFRGLGYLL
                     EAVGD"
     gene            15625..17016
                     /locus_tag="VFF22_19100"
     CDS             15625..17016
                     /locus_tag="VFF22_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HZX18961.1"
                     /translation="MHEPGSLRGRLIRRLALLLALILLASSLSAYWSARRAADTAYDR
                     TLLASARAIADGLYTTADRLSANVPYVALDTFAYDSAGRIYFQVLDVQGALVSGYEDL
                     PPAPPGTKRTEDYPALAHFYDAEYRNQGVRVVSLLQPVSDPGVNGVAEIRVAETQGAR
                     ERMARELLLGTLLRMGVLSLSALLLVWLAVSAALRPLEALRRSVAARTSDDLQPLSDV
                     GMPRELRPLIDALNQFNDRLRSLFERQSQFIADASHELRTPLAALKARVELGLRAQEP
                     TIWRSTLEEAAQNTDKLTHLANQLLSLARIESGARAIAEGGAQRLDLSQLARELGMAL
                     APLAHARGISLALEAEQPVWLSGEPTLLNELLCNLLDNALAHTAAGGNVILRVLPTGV
                     LEVEDDGPGIPPEEHERVFARFYRRSNKGSGAGLGLAIVGEICRAHQAEISLHQAQPQ
                     GLLVRVAFASAEA"
     gene            complement(17018..17779)
                     /locus_tag="VFF22_19105"
     CDS             complement(17018..17779)
                     /locus_tag="VFF22_19105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX18962.1"
                     /translation="MRTLLLSVLFSLFCGGLAAADSVRLTNGEWPPYLGEQLPHKGVA
                     SRIVAEAFALQGIDVAWEFHPWARSLQLAERGKRDGSAVWLHNSKREEKFFISDPVVE
                     SGYYLFHRKGFSFDWKQVVDLQGRRLVGTRGYDYGEDFQQAEASGQLRVNRVTSDETA
                     FRQLLAGRVDLFPMDKVVGFDMLHQHFSAADRAQLSFHPKPLRSDSLHLLLSRQVPGN
                     AELIARFNQGLAQLRESGKIAQYLLEIQQPLSLAP"
     gene            complement(17848..18684)
                     /locus_tag="VFF22_19110"
     CDS             complement(17848..18684)
                     /locus_tag="VFF22_19110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HZX18963.1"
                     /translation="MSKLAEKVQKELIQAIDNDELVLPTLPEVALKVREAAEDPDIGI
                     AQLSKVIGNDAALTARIIKVVNSPLLRTNKEITDLQMAVGRLGINYTCNLATGLAMEQ
                     MFQATSDVVDRKMREVWNKSTEIAGICHVLCKHYTRLLPDQATLAGLVHQIGVLPILT
                     YAEDHSELLADSISLNHVIEQIHPIIGDKILRTWEFPELIAMVPGQYLDFTRDSAKVD
                     YVDIVQVATLQSYIGSEHPYTKLDWSQIPAFAKLGLDPSQDLNEDEDLSAAMEAAMSM
                     LQ"
     gene            complement(18734..19675)
                     /locus_tag="VFF22_19115"
     CDS             complement(18734..19675)
                     /locus_tag="VFF22_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HZX18964.1"
                     /translation="MADTAFYCTLSHEGILAVRGPDANKFLQGQVTCNLNYLSDSLSS
                     LGARCTPKGRMQSSFRIVSVSDGYMMAMASDLLEAQLTDLKKYAVFSKSKLTDESQDW
                     LRIGLSGGDDALRSLGLELAETTDSVARSNELLALRLSDGRAELWIPASQAAVVQAQL
                     ASQLPEVPLNDWLLAQVRAGIGQVMGSTRELFIPQMINLQAVGGVSFKKGCYTGQEIV
                     ARMQYLGKLKRRLYRLELHDSQVPEPATALFSPTHTSSVGEVVLAARSPAGVELLAVL
                     QDEAALNGVIHLGSAEGSTLSVLDLPYTLDSDREIQR"
     gene            19850..20104
                     /locus_tag="VFF22_19120"
     CDS             19850..20104
                     /locus_tag="VFF22_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HZX18965.1"
                     /translation="MVDSVELNRLFWHSRRGMLELDVLLVPFVREVYPHLDEADRERY
                     RKLLECEDQDMFGWFMQRGEPEDADLKRMVRMILDRVQPQ"
     gene            20088..20540
                     /locus_tag="VFF22_19125"
     CDS             20088..20540
                     /locus_tag="VFF22_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019647984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HZX18966.1"
                     /translation="MSSPSDAFESQWQPSRQLLVCYALIQGLALLSLVLVDIPLWARL
                     LGIGFCLAHAAWVLPRHLLLGSSSAYRALRFTAEGWQVHSAAQGWQAIELHPDSLALP
                     LIVLLRFRLAGQRRVRALCIARDSLPRDQHRRLRVRLKFSRHRWAAAE"
     gene            complement(20509..22125)
                     /gene="nadB"
                     /locus_tag="VFF22_19130"
     CDS             complement(20509..22125)
                     /gene="nadB"
                     /locus_tag="VFF22_19130"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HZX18967.1"
                     /translation="MSQHYQHDVLVIGSGAAGLTLALTLPEHLRIAVLSKGNLSNGST
                     YWAQGGVAAVLDDTDTIESHVEDTLNAGGGLCREDAVRFTVEHSREAIQWLIDQGVPF
                     TRDDEHAREDGGFEFHLTREGGHSHRRIIHAADATGAAIFNTLLEQTRQRSNIELLEQ
                     RVAVDLITERKLGLDGQRCLGAYVLDRSTGEVDTFSARFVILATGGAAKVYLYTSNPD
                     GACGDGIAMAWRAGCRVGNLEFNQFHPTCLYHPKAKSFLVTEALRGEGALLKLPNGER
                     FMPRFDERAELAPRDIVARAIDHEMKRLGIDCVFLDISHKPADFVKSHFPTVYERCLD
                     FGIDITKEPIPVVPAAHYTCGGVLVDQSGRTDIPGLYAIGETSFTGLHGANRMASNSL
                     LECFVYARSAAENILAQLDQISMPSDLPGWDASQVTDSDEDVIIAHNWDELRRFMWDY
                     VGIVRTNKRLQRAEHRVRLLLDEIHEFYSNYKVSRDLIELRNLAQVAELMIRSAMLRH
                     ESRGLHYTLDYPQQLAEAKDTILLPPTYGD"
     gene            22493..23074
                     /gene="rpoE"
                     /locus_tag="VFF22_19135"
     CDS             22493..23074
                     /gene="rpoE"
                     /locus_tag="VFF22_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HZX18968.1"
                     /translation="MLTQEDDQQLVERVQRGDKRAFDLLVLKYQHKILGLIVRFVHDT
                     HEAQDVAQEAFVKAYRALGNFRGDSAFYTWLYRIAINTAKNHLVSRGRRPPDNDVSAE
                     DAEFYDGDHALKDIESPERALLRDEIEATVHRSIEQLPEDLRTALTLREFDGLSYEDI
                     ASVMQCPVGTVRSRIFRAREAIDRSLQPLLQET"
     gene            23106..23702
                     /locus_tag="VFF22_19140"
     CDS             23106..23702
                     /locus_tag="VFF22_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003184342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RseA family anti-sigma factor"
                     /protein_id="HZX18969.1"
                     /translation="MSRETLQESLSAVMDNQADELELRRVLAASDDPEIRATWSRYQI
                     ARAVMHKELLEPRLDIAAAVSAALADEAAPVAPQVQQRGPWRTVGRLAVAASITVAVL
                     AGVRLYNQDDITGAQLAQQSTQPALNIPQVNGPAMLAGFNTSEEQSAPAASAVGQGQP
                     GWHEQRLPAYLRQHAQQAAMSTSEGALPYARAASLENR"
     gene            23725..24672
                     /locus_tag="VFF22_19145"
     CDS             23725..24672
                     /locus_tag="VFF22_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008153075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HZX18970.1"
                     /translation="MRFIPLSVLLLGSWLALPAAADEGRALLQRLADAERTQSFQGAF
                     VYERNGSFSTHSIWHRVEENGKLRERLLQLDGSAQEVIRIDGQVQCASGALADQVVDS
                     QLWPARTLNAEQLSEWYELRVLGKSRVAGRVTSVLALVPRDQHRYGIELHFDQQTGLP
                     LKSLLVNDKGQLLERFQFTQLETVAPAASNLEPGAGCRPVRLAPSPVDTVEAWRSDWL
                     PPGFSLGSVTQRRSPASNESVACQVYEDGLARFSVFIEPLRDAAVEDVRTQLGPTVAV
                     SRRLKTDDGDVMVTVVGEIPLGTAERVALSMRSAAEVVQ"
     gene            24669..25124
                     /locus_tag="VFF22_19150"
     CDS             24669..25124
                     /locus_tag="VFF22_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HZX18971.1"
                     /translation="MIEEQGRVVAVEPGAVWVETLRKSTCSSCSVKAGCGQGLLDQLG
                     ASGRRGYVRALSNLQLNVGDAVIIGVREDLLVRGSLLVYLLPLLGLFTAAVLAEQMGL
                     NEPWVILSALFGFLFACCAVRWRSRVTAADPALQPVVLRALLGSVATAF"
     gene            25153..26589
                     /locus_tag="VFF22_19155"
     CDS             25153..26589
                     /locus_tag="VFF22_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011059790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HZX18972.1"
                     /translation="MSKPSLKSSFTALFAVLLMGQALFAQASLPDFTELVEQASPAVV
                     NISTRQKMPDRTVAGSGQLNMPDLEGMPPMFREFFERNIPQVPRAPGGGRQREAQSLG
                     SGFIISADGYVLTNNHVIADADEIIVRLSDRSELEAKLIGTDPRSDVALLKVEGAGLP
                     TVKLGKSEELKVGEWVLAIGSPFGFDHSVTAGIVSAKGRSLPNESYVPFIQTDVAINP
                     GNSGGPLFNLAGEVVGINSQIFTRSGGFMGLSFAIPMSVAMDVADQLKAEGKVSRGWL
                     GVVIQEVNKDLAESFGLDKPAGALVAQVLENGPAAEGGLQVGDVILSLDGKPIIMSAD
                     LPHLVGALKPGTTAELDVVRDGARKKLKLNIGALPEDGEALAGNPAPGVERSSNRLGV
                     KVVELSAEQKKSLDLKGGVVISEVQEGPAALIGLRPGDVITHLNNQAINSAKTFAEVA
                     QALPKNRSVSMRVLRQGRASFITFKLAD"
     gene            26751..28550
                     /gene="lepA"
                     /locus_tag="VFF22_19160"
     CDS             26751..28550
                     /gene="lepA"
                     /locus_tag="VFF22_19160"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HZX18973.1"
                     /translation="MSDLSHIRNFSIIAHIDHGKSTLADRFIQMCGGLADREMEAQVL
                     DSMDLERERGITIKAHSVTLYYKAKDGITYQLNFIDTPGHVDFTYEVSRSLAACEGAL
                     LVVDAGQGVEAQSVANCYTAIEQGLEVMPVLNKIDLPQADPDRVKEEIEKIIGIDATD
                     AVACSAKTGLGVDEVLERLVHTIPAPTGDIEAPLQALIIDSWFDNYLGVVSLVRVRHG
                     RIKKGDKVLVKSTGKVHLVDSVGVFNPKHTATVDLKAGEVGFIIASIKDIHGAPVGDT
                     LTLAATPNVEVLPGFKRIQPQVYAGLFPVSSDDFEDFRDALQKLTLNDSSLQYSPESS
                     DALGFGFRCGFLGMLHMEIIQERLEREYDLDLITTAPSVIFEVVLKTGETIYVDNPSK
                     LPDVSSVEDFREPIVQATILVPQEHLGNVITLCIEKRGVQRDMQFLGSQVQVRYDLPM
                     NEVVLDFFDRLKSTSRGYASLDYHFDRYQSANLVKLDVLINGDKVDALALIVHKDNAH
                     YKGRALTEKMKELIPRQMFDVAIQAAIGGQIVARTTVKALRKNVLAKCYGGDVSRKRK
                     LLEKQKAGKKRMKQVGNVEIPQEAFLAVLRLDS"
     gene            28557..29411
                     /gene="lepB"
                     /locus_tag="VFF22_19165"
     CDS             28557..29411
                     /gene="lepB"
                     /locus_tag="VFF22_19165"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZX18974.1"
                     /translation="MSINFPLLLVIAVAVCGALALFDLIFLAPRRRAAISTYHGQVSE
                     PDEAVVERLNKEPLLVEYGKSFFPVLAIVLVLRSFLVEPFQIPSGSMKPTLEVGDFIL
                     VNKFAYGIRLPVLDTKVIEVGDPQRGDVMVFRYPSDPNINYIKRVVGLAGDRIAYSSD
                     KRLTINGESVAQQLIGDEPGSLGSATLYSEKLGETEHQIRKEMQRYRVEPGREWVVPQ
                     GHYFMMGDNRDNSNDSRYWNDPAIPKPLWGMVPDQNIVGKAFAIWMSWPDPKSRNLPN
                     FSRVGLIH"
     gene            29516..29893
                     /locus_tag="VFF22_19170"
     CDS             29516..29893
                     /locus_tag="VFF22_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HZX18975.1"
                     /translation="MKSAHSQKGMSILSWLMVLAVIAFLASAAFKVLPHYFDYMSLEK
                     MITSVETDKAAEVRSINEFYSHISKGMQVNSIRDLNMQDAIKVKIENNEFRVHMKYEK
                     REPLIENIDLVVRFDKEFRVRMP"
     gene            29890..30579
                     /gene="rnc"
                     /locus_tag="VFF22_19175"
     CDS             29890..30579
                     /gene="rnc"
                     /locus_tag="VFF22_19175"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HZX18976.1"
                     /translation="MSASLARLERQLGYTFKDQDLMILALTHRSFAGRNNERLEFLGD
                     AILNFVAGEALFQRFPLAREGQLSRLRARLVKGETLAILARGFDLGEYLRLGSGELKS
                     GGFRRESILADALEALIGAIYLDTGMDAARERVLAWLTTELDSLTLVDTNKDPKTRLQ
                     EFLQSRGCELPRYEVADIQGEPHCRTFVVECQITLLNDKTLGQGASRRIAEQVAAAAA
                     LIALGVENGND"
     gene            30572..31474
                     /gene="era"
                     /locus_tag="VFF22_19180"
     CDS             30572..31474
                     /gene="era"
                     /locus_tag="VFF22_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017734281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HZX18977.1"
                     /translation="MTDSPVTRCGYVAIVGRPNVGKSTLLNHILGQKLAITSRKPQTT
                     RHNMLGIKTEGEVQAIYVDTPGLHKNNEKALNRYMNKNASSALKDVDVVIFVVDRTRW
                     TDEDQMVLERIQYVEGPVILAINKTDRIEDKAELMPHLEWLATQLPKAEIVPVSAQHG
                     HNLDTLEKLVGEHLPEGVHFFPEDQVTDRSSRFFAAELVREKIMRQLGAELPYQITVE
                     IEEFKRDGRILHIHALILVERDGQKKIIIGDKGERIKRIGQEARKDMEVMFDSKIMLN
                     LWVKVKSGWSDDERALRSLGYDNI"
     gene            31519..32217
                     /gene="recO"
                     /locus_tag="VFF22_19185"
     CDS             31519..32217
                     /gene="recO"
                     /locus_tag="VFF22_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZX18978.1"
                     /translation="MLALGQPAYVLHSRAYRESSALVDFLTPQGRLRAVLRGARGKAG
                     SLARPFIPLEAEFRGRGELKNVGRLDPAGIPNLLVGEALFSGLYLNELLIRLLPAEDP
                     HPAVFEHYAMTVLALAQGRALEPLLRSFEWRLLDELGYGFALDLDSEGQPIAETGLYR
                     LQTETGLVPVGHLQPGVFQGAELLAMAEADWTAPGALAAAKRLMRQALAPHLGGRPLV
                     SRELFMTLKEPSRD"
     gene            32210..32956
                     /gene="pdxJ"
                     /locus_tag="VFF22_19190"
     CDS             32210..32956
                     /gene="pdxJ"
                     /locus_tag="VFF22_19190"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HZX18979.1"
                     /translation="MTDANRILLGVNIDHVATLRQARGTRYPDPVKAALDAEEAGADG
                     ITVHLREDRRHIQERDVRLLKEVMQTRMNFEMGVTEEMLAFAESIRPEHVCLVPETRQ
                     ELTTEGGLDVAGQEARIRAAVERLSKIGCEVSLFIDPDLQQIEASKRVGAPAIELHTG
                     RYADAHTPEEAARELARIRDGVACGLQHGLIVNAGHGLHYHNVEPVAAIAGINELNIG
                     HALVAHALFVGFKQAVAEMKALVVAASGRG"
     gene            complement(33015..33917)
                     /locus_tag="VFF22_19195"
     CDS             complement(33015..33917)
                     /locus_tag="VFF22_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZX18980.1"
                     /translation="MNIQPIASAPTLQPLLESDVLRTFVGIAESGSFTRTAAQVYRTT
                     AAVSMQIKRLEETLGRNLFVREARRVRLTPDGEMLLGYARRLLKLNEEAVAHFLAPAL
                     TGRVGFGSPTDLGSRVLPGVLSMFARSHPAVQVDVAVGRSADLIEKLDAGELDLILIN
                     AGNDGQDDSRGEIIYSEALVWAGREGGLAVQRSPLPLALAYPGCAWRRTALDALDRLD
                     LPYRIAYSCEQCAGQESAMIADLAVAPFPRSLVRSPLRRLGEEHGLPPLGDYHIKLLR
                     GSNRGDAAAALAVQVLAAFRDQHN"
     gene            34135..34356
                     /locus_tag="VFF22_19200"
     CDS             34135..34356
                     /locus_tag="VFF22_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18981.1"
                     /translation="MPAMWPMDLQNAIVDWLGAWRWRRQMRHLLQLEARGLARLGDAR
                     DAVSQGAAAPLREIAARRAARRRGDDRQQ"
     gene            complement(34357..35325)
                     /gene="cmoB"
                     /locus_tag="VFF22_19205"
     CDS             complement(34357..35325)
                     /gene="cmoB"
                     /locus_tag="VFF22_19205"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HZX18982.1"
                     /translation="MISRLDLDALQAQLAGTPLQDWAADLPGQIDAKLAVGHGDLPRW
                     YGAVQALPELNVEQLELLHSFSFNGHCDEPTRAALKTALQGLIPWRKGPFHLFDVQVD
                     TEWRSDWKWQRVAPYLDLKGKRVLDVGCGNGYYMWRMLGAGADSVVGVDPNWLFLCQF
                     LAMKNYLPDLPAWHLPLAFEELPGKLQGFDTVFSMGVLYHRRSPIDHLIDLKDCLVKD
                     GELVLETLVVEGDAQQVLVPEDRYAQMRNVWFLPSVPALELWLRRAGFVDVRCVDVST
                     TSVDEQRATEWMRFQSLPEFLDPADHSRTIEGLPAPCRAVLVARKP"
     gene            complement(35322..36098)
                     /gene="cmoA"
                     /locus_tag="VFF22_19210"
     CDS             complement(35322..36098)
                     /gene="cmoA"
                     /locus_tag="VFF22_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019407925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HZX18983.1"
                     /translation="MPGFIDQCAAIVSKQPDRLFAQPLAQVPDFVFNEDVVRVFPDMI
                     KRSVPGYPTIVENIGVLAGRFAQPHSVLYDLGSSLGAVTQALRRHVQIEDCWVIAVDN
                     SSAMVERCREYLHAQDSMFQELLPVEVIEADILALEFQPTSLVALNFTLQFIQPEQRL
                     PLLSRIRQALLPGGALILSEKLRFEDQAQHDLLGELHIAFKRANGYSELEIAQKRSAI
                     EKVMLPDSLEQHRERLLAAGFSQVVPWFQCLNFASLVALP"
     gene            complement(36138..36803)
                     /locus_tag="VFF22_19215"
     CDS             complement(36138..36803)
                     /locus_tag="VFF22_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HZX18984.1"
                     /translation="MRYLLLGLLGAAVFLYARLSGLSELGLLSKGIPVIALLLWLRTA
                     PSGLYRRWLSLGLLCSLAGDILLDWPDDLFVFGLGAFLLAHLAYLRAYLSDCTQPAWR
                     ALLLALATGGTMFAILASANLGSLLIPVACYALAISAMLWRALARLDQPGLNRSSTRL
                     AAAGAALFVLSDSLIGINRVVAPFEAAPLAIMLSYWLGQWAIAASAFKQSAEPSPMQI
                     CDQ"
     gene            complement(36900..37295)
                     /locus_tag="VFF22_19220"
     CDS             complement(36900..37295)
                     /locus_tag="VFF22_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HZX18985.1"
                     /translation="MFAPRLTVALSLALLAACAQKPSSVTLEQSQQSKCPLSLHVGQQ
                     FNLRLPSNPTTGFRWLLRDSAPQVLKSLGPEVYSNPEDAGLVGSAGVSTWRFEVITAG
                     EGQLALTYERPWEVGVVPAQRFNCTIKAR"
     gene            complement(37336..38070)
                     /locus_tag="VFF22_19225"
     CDS             complement(37336..38070)
                     /locus_tag="VFF22_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX18986.1"
                     /translation="MALSIALALALALLLAGHFLSQLAGKRHQQHLNELQAALLQRAI
                     DLLQNLQKHRGLGAQQDLPSTSQRNALARQLDQLWLNWPGASLALAPLQQDWPRLRRN
                     PADFAAHCQVIDGLLAVIATLETRLHQTGNAHVEGIAQACHGLENLARLRGLSVRAAN
                     YSQCPPSLKQQIQQLCLELEQSPVGASLQQVLQRLEHELIDSRDVRLAPHECFALLTP
                     LIDDYAQRLRAPLQAAFAARAPAEQP"
     gene            38321..39073
                     /locus_tag="VFF22_19230"
     CDS             38321..39073
                     /locus_tag="VFF22_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZX18987.1"
                     /translation="MFNARLMRLDDQAHEHTHDHHQLVLSLAGRAEFEVNGRGGEVCR
                     MRACLVPGDADHQFAGMGDNRMLIIDLDEQGTSSEDLQLLTRLFETPRYPQLDADFQN
                     LLSYAGAELERYGTDPLLPRALGGVLLRALHLRLFGEPHARPVGALNLERIDSYIVKH
                     LARRISVAELAQVACLSPSHFHAQFKDSVGLTPHQYLLRTRLDRAVRLLRESGLPLIR
                     IAEECGFSSQSALTTAMRRYLGLTPKRLRSAE"
     gene            39240..39926
                     /locus_tag="VFF22_19235"
     CDS             39240..39926
                     /locus_tag="VFF22_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017902455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine utilization protein"
                     /protein_id="HZX18988.1"
                     /translation="MSKHLHLLFAFSLLGILLFSAPLHATRLQAELVDDQGQPLANAV
                     LSLRSAVAPEVAPAAAVMDQRSQQFAPNVLAVRSGTSVSFPNSDNIRHHVYSFSLAKR
                     FELRLYQGTPSEPVIFDKPGVVVLGCNIHDWMVGYVYVTDDPWFAVSDAQGKLNLEQL
                     PAGAYTVSLWHPQAPDMLPQAAGQVQLTEAGSQQRFTLAMQAPAIQAAPSAPASAFGD
                     AFKKARDAAQ"
     gene            39913..42249
                     /locus_tag="VFF22_19240"
     CDS             39913..42249
                     /locus_tag="VFF22_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZX18989.1"
                     /translation="MLRNSFQARIAGVLVLLLLVVIGALYAAVQTATSAAVRSQAREQ
                     LDVGSLVFQQLLEVRGRQLHDAVQVLAADFGFKDAVASGDTETIRSALANHGARINAS
                     AVMMLGLDGNLEVSSDRQISGETAQRLSAQVMQRQREGVQIFLMPIADKIYLLVQATV
                     TAPLPIARVMMGFQVDDVFAAELRELTHLDLTLLATQDGAADIWISTLQPAQREQLQG
                     SMAELANDAVSLIGDESYLNQTLVLASGDGFEVRALLHKSLSQAQQAFAPLDQNILLI
                     ALVALTASLGGALLLARSVSQPVQQLAAVAERVRQGDYQASLDLQRGDELGRLATAFK
                     AMQQGIAERERQLAHNALHDALTGLPNRTLALERLGSAITAGRPTALLYLGVANFRAV
                     NESLGAEGGDLALQQLSRRLQAILRPGDSVARIIGDEFLLLLENTDSDSAVGIADKVQ
                     QLLLKPLRVATHDFALDCRIGIASYPVDGNAPEELLRRASIAMQDAAGMPSHLQVYQR
                     GRDDAQQRQIRLIRDLRHAPGKAELLLHYQPKLDIAAGQVRQAEALLRWQHPELGMVS
                     PGEFIPLAERTGSIQLLTTWVIEEVLRQLREWAGRGLCVQVSLNISTEDLIDPQLPAR
                     VSKLLAEYQVPAEQLIFEITESGVMLNPELALQVLHDLRACGISLSVDDFGTGYSSLA
                     QLKRMPVQELKIDQSFIRDLDDASEDSVIVRSTIEMSHSLGLKVVAEGVEFERSLQLL
                     GRWQCDTAQGYLISRPLAAQAFETWMAQYTHASSTLVH"
     gene            42252..43109
                     /locus_tag="VFF22_19245"
     CDS             42252..43109
                     /locus_tag="VFF22_19245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HZX18990.1"
                     /translation="MTQLPRCIALLLGLLANLAWADGRLLATGGASSIEGAAGGGITP
                     WAVLSGYGERGEWGADVFATRVETGDYRLDVAGVGVAFDNRIELSYARQRFDLGTLAR
                     DLNLPENSLSQDIFGLKLRLFGDLIYDQLPQVSLGIQHKRQKDFLIPSLVGAQRSEDT
                     EGYISASRLILGGAFGYNLLLNGGLRYSRANELGLLGFGGDRRDRHSVLKEGSVAVLF
                     NPRWALGVEYREKPDNLSFAGESDWADLFLGYFPNKNLAVVLAYARLGEIATLDNQDG
                     VYLSVQGSF"
     gene            43112..43531
                     /locus_tag="VFF22_19250"
     CDS             43112..43531
                     /locus_tag="VFF22_19250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005739179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HZX18991.1"
                     /translation="MRSLLLVVVLSLAACAQQPAKDDSLYRDLGEQAGITRIVEGMLL
                     NIAADPRIVRHFENIDIVRLRDKLVEQICVEAGGPCTYTGDSMEESHKGQNLTPSDFN
                     ALVENLQDAMSAQGVPMPAQNRLLARLAPMRAQVIDR"
     gene            complement(43576..44442)
                     /locus_tag="VFF22_19255"
     CDS             complement(43576..44442)
                     /locus_tag="VFF22_19255"
                     /inference="COORDINATES: protein motif:HMM:NF019974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HZX18992.1"
                     /translation="MDGLHGFTLLACVATLTVIGLALYGMHRRLLRQKQARIQTNIRQ
                     LVLLRALLADLQRHRGLSHAVLCGDTSMSQDLANMRQQLDRHIRDAQTLSSNQREAWN
                     SLIDHWSRLREGHSADPANNLAQHHLIIRNSIFLMEDVASEVDLSEGRAALAYLPCIW
                     REVVQAAEWAGQARALGTGIAAAGQSNVEQRVRLRFLYQKIQLLADTAFATLHRHTAE
                     HPQRDAFRLAQREQVVTSFLYCIEQELLGQEQPHIAAKSYFQRATQAIDELLALVDTA
                     LAQLQPEQNSRY"
     gene            complement(44448..45581)
                     /locus_tag="VFF22_19260"
     CDS             complement(44448..45581)
                     /locus_tag="VFF22_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019689261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX18993.1"
                     /translation="MNWLTYPALLLLRALGVVGMQCSLLGITLVGIATTVLCPTHFAA
                     SIWALLAYLSIASLYLLWREVLQLQRYCDASVQQTGRQAPRTNEWQLLQPIGRSLQQC
                     LQREQRQQQLLQQRLDEIAHSSQELEKSAELVTRNAGNQSDSASMAAAAVEQLNVSIV
                     QVAALADESRQTSLVASEQLTDGIDRLTELVQQVAEMTRQAQATNQLIQQLNSNSHTI
                     NEMSGTIRSIADQTNLLALNAAIEAARAGESGRGFAVVADEVRRLAIHSQESAAEIGR
                     NIHSVQQHIQGATLQVANLSELAQRGAQSSEAVRHLLNQVQQCTRRLTEQVDQVAVST
                     EQQGKAVAEIAELADHVRQGNADNLRAADQARSIAHHLAHLTG"
     gene            complement(45880..46737)
                     /locus_tag="VFF22_19265"
     CDS             complement(45880..46737)
                     /locus_tag="VFF22_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HZX18994.1"
                     /translation="MQSEASDYSIYRRVVSQLMQGEEQLPSLPTITLDIRRALGDSNI
                     SLNALVRLISRDPALSAMLMKHASSALYRHSLAPKTLREVIGLLGMTEVDRITMVHSI
                     KSLFTLHSPGHKRLFVDAWERLVIKASTSAMLARLLGHVTAEHALLASLLSEVGTLAV
                     LSAFRNESVTPSSELYYKLCREYSKSLGIIVLKKWSVHNEYIEIIRDTGNWQRSPGHS
                     LQLLDLVNLALYHAIKERTPDADLPPLQELAAYQKLMPPQDFIGENGELSLVISHRAD
                     ILAIAQTLR"
     gene            47272..48759
                     /gene="putP"
                     /locus_tag="VFF22_19270"
     CDS             47272..48759
                     /gene="putP"
                     /locus_tag="VFF22_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HZX18995.1"
                     /translation="MTANTPMLVTFVVYIALMVLIGLFAYMRTKNLSDYILGGRSLGS
                     FVTALSAGASDMSGWLLMGLPGAVYLSGLSEGWIAIGLIVGAYLNWLFVAGRLRVQTE
                     HNGNALTLPDYFTHRFEDSSRVLRIFSALVILVFFTIYCASGVVAGARLFESTFGMSY
                     ETALWAGAAATIAYTFIGGFLAVSWTDTVQATLMIFALILTPIVVLIATGGADATFAA
                     IELKDATSFDMLKGATFVGVISLMAWGLGYFGQPHILARFMAADSVKSIPAARRISMT
                     WMILTLAGAVAVGFFGIAYFSANPDLAGPVTENPERVFIELAKLLFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSALTEDFYKAFLRKDASQVELVWVGRGMVLLIAIVAIMLAAN
                     PENRVLGLVSYAWAGFGAAFGPVVILSLVWKQMTRNGALAGMLVGAMTVILWKNFLGH
                     LGLYEIIPGFIFATIAILVFSKIGSGPSASMIKRFDEAEKEYQSV"
     gene            48990..49211
                     /locus_tag="VFF22_19275"
     CDS             48990..49211
                     /locus_tag="VFF22_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010220998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HZX18996.1"
                     /translation="MTQSPKNPLHGVTLEAILTALVAQYGWDGLAERIDIRCFKSDPS
                     IKSSLTFLRKTPWAREKVEALYIRSQARK"
     gene            49306..49635
                     /gene="fliE"
                     /locus_tag="VFF22_19280"
     CDS             49306..49635
                     /gene="fliE"
                     /locus_tag="VFF22_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HZX18997.1"
                     /translation="MSQGVEFNRLMLEMRSMQADAMARQKPVTSTPEPGAPSFSEMLG
                     QAVGKVNETQQASNKLATAFEMGTSGVDLTDVMIASQKASVSMQAMTQVRNKLVQAYQ
                     DIMQMPV"
     gene            49651..51435
                     /gene="fliF"
                     /locus_tag="VFF22_19285"
     CDS             49651..51435
                     /gene="fliF"
                     /locus_tag="VFF22_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HZX18998.1"
                     /translation="MAEAVVANVPATTGGDDPKKPLFGLTFLENLADMSMLRQIGLLV
                     GLAASVAIGFAVVLWSQQPDYQPLYGSLDGMDASQVMETLSAADIKYTVEPNSGALLV
                     KSGDLARARMRLAAAGVAPKDNSVGFEILDQEQGLGTSQFMEATRYRRGLEGELARTV
                     SSLNNVKAARVHLAIPKSSVFVRDERKPSASVLVELYSGRGLEPSQVMAIVNLVASSV
                     PEMDKAQVTVVDQKGNLLSDIQEMSELTMAGKQFDYSRRMESLFTQRVHNILQPVLGS
                     GRYKAEVSADVDFSAVESTSEMFNPDQPALRSEQSVNEQRTSSLPPQGVPGALANQPP
                     GAAAAPEQAGGAAAVPGPVAAGQPLLDVNGEQIIDPATGLAMLAPYPADKREQSTRNF
                     ELDRSISYTKQQQGRLRRLSVAVVVDDQVKIDAASGETTRVPWSSDDLARFTRLVQDS
                     VGFDASRGDSVSVINTPFSAQLGEEMIEIPFYTQPWFWDVVKQVLGVLFILVLVFGVL
                     RPVLNNITGGGRGQGDGRGGDVELGEMGALGGDLAEDRVSLGGPQSILLPSPSEGYDA
                     QLNAIKGLVAEDPGRVAQVVKDWINADE"
     gene            51428..52444
                     /gene="fliG"
                     /locus_tag="VFF22_19290"
     CDS             51428..52444
                     /gene="fliG"
                     /locus_tag="VFF22_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HZX18999.1"
                     /translation="MSDNRTATKLSKVDKAAILLLSLGETDAAQVLRHLGPKEVQRVG
                     VAMAGMRNIQREQVEQVMGEFVDIVGDQTSLGVGADGYIRKMLTQALGEDKAGNLIDR
                     ILLGGSTSGLDSLKWMEPRAVADVIRYEHPQIQAIVVAYLDPDQAGEVLGHFDHKVRL
                     DIVLRVSSLNTVQPAALKELNQILEKQFSGSSNTTRAALGGVKRAADIMNFLDSSVEG
                     QLMDSIREVDEDLSSQIEDLMFVFDNLADVDDRGIQALLREVSSDVLVLALKGADEAI
                     KEKVFKNMSKRAAELLRDDLEAKGPVRVSDVETAQKEILTIARRMAEAGEIVLGGKGG
                     EEMV"
     gene            52448..53278
                     /gene="fliH"
                     /locus_tag="VFF22_19295"
     CDS             52448..53278
                     /gene="fliH"
                     /locus_tag="VFF22_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HZX19000.1"
                     /translation="MSSKEPPSELIRAKDLKGVDVWALPSFDPEVEIPEPEEQQASEQ
                     PVDTGEQVPAESEEVALEDVKPLTLDELEAIRQDAYNEGFATGEKDGFHAGQLKAKQE
                     ADIALAAKMAGLEQLMTQLFEPIAGQDQQLEEAMVGLLGQMVRQVIQRELVSDSSQIR
                     QVLSEALKLLPMGTGNIRIHLNPQDFEMIKALRERHEESWRILEDASLLPGGCRVESE
                     LSRIDATVETRLNQAIKQLFEQQREQASHPLEADLHTDLDALPVDAPQVNSQASPDAP
                     "
     gene            53268..54566
                     /gene="fliI"
                     /locus_tag="VFF22_19300"
     CDS             53268..54566
                     /gene="fliI"
                     /locus_tag="VFF22_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HZX19001.1"
                     /translation="MRLDRVSFAKRLAGYSEAVALPTQPLVEGRLLRMVGLTLEAEGL
                     RAALGSRCLVINDDSYHPVQVEAEVMGFSNGKIFLMPVGSLAGIAPGARVVPLPDTGR
                     LPMGMSMLGRVLDGTGRALDGKGGMKAEDWVPMDGPAINPLNRHPISEPLDVGIRSIN
                     GLLTVGRGQRLGLFAGTGVGKSVLLGMMTRFTEADIIVVGLIGERGREVKEFIDEILG
                     HEGLKRSVVVASPADDAPLMRLRAAMYCTRIAEYFRDKGKNVLLLMDSLTRFAQAQRE
                     IALAIGEPPATKGYPPSVFARLPKLVERAGNAEKGGGSITAFYTVLSEGDDQQDPIAD
                     AARGVLDGHIVLSRRLAEEGHYPAIDIEASISRVMPQVVSAEHMRLAQRFKQLWSRYQ
                     QSRDLISVGAYVPGGDADTDLAIARQPAMVKYLRQNLQDN"
BASE COUNT        10250 a        15626 c        17489 g        11217 t
ORIGIN      
        1 gtggagcggc ttgtccaccg agggtggcgg tggacaaggc tgcgccatgt ccaccctacg
       61 gattatttat tgcgccactg cgggtcgcgt ttttccagga aggcgttgac cccttcgcgg
      121 gtgtcgtcgg cgtcgaacag atcgacaaaa cgctcgcgct cttcactcag ccaggtattc
      181 gggctgcgtt cgcgggcgcc ctggatcagc ggtttgatgg tgcgcaccgc caccgggctt
      241 tgccgcgcga ctttcgctgc caggagcagg gcatgaccac gggactcgcc gctatcgacc
      301 acctgctcga ccaggccgat gcgcagcgcg gtttccgcat cgacgcgttc gccgcagagg
      361 atcatgcgct tggcccagcc ttcgccgatc agccagctca agtgttgggt gccgccggcg
      421 cagggcagca ggccgaccgt ggcttctggc agggccattt gcgcctggcg ctcggcgatg
      481 cggatgtcgc aggccagggc acattccagg ccgccgccca tggcgtagcc gttgatcgcg
      541 gcaatcgata cgccacggaa atcacgcagc gcttcgaagg cctcgccgaa gcgccgggcc
      601 atttcgcggg cgcgggcctt gtcgccatcg gcgaacagct tgaggtcggc gccggcgctg
      661 aagaacttgc caccctggcc ggtaatcacc agggcgtaaa tgtcatcgtc acggttgaga
      721 tgttcaatca gcagcttgag gccgatcagc gagtcgcggt cccaggtgtt ggccggcggc
      781 tggttgatgg tgatcagcgc ggtattgccg tgcttttcca cggtgagctt gtgggtcagg
      841 tcgaatacgc ccgggtggta ggcttcgatg gcgtggctca taacaatcct cgtaaataca
      901 ttttgtctga tggtacccac gctctgcgtg ggtacccagc ctgggacgct ctgcgtcccg
      961 cttgcccccc tggagggacg cggagcgtcc ggggcggcat tcccacgcgg acgcttcaac
     1021 gcctcgacgt gggaacgatc aggtgtgcag ggcgactccg cccctcaccc taaccctatc
     1081 cccggagggg agaggggata aaagctacag caagcgatcc agcatgccgc cctgatccag
     1141 caggcggcgg gcgatgatca cccgcatgat ctcgttggtg ccttcgagta tctggtgcac
     1201 gcggctgtcg cgcacccagc gctccagcgg gtagtcgttc agatagccgt aaccgccatg
     1261 cagctgcagc gcttcgttgc atagggtgaa gcagtgatca gtggcaaagc gcttggccat
     1321 ggcgcaatac aggctggcct cgctgtgacc atggtccagt ttgtgcgcgg ccaggcgcac
     1381 catctgccga ctggccgtca aatcggtgag catgtcggcg agcttaaatt gcagtgcttg
     1441 gaaatcgctc agcggcttgc cgaactgttt gcgttcctcg acatagcgca gcgattgctc
     1501 cagtgccgcc tgagcagcgc cgagtgagca gctggcgata ttgatgcgcc cgccatccag
     1561 gcccttcatc gcgtagacga agccctggcc ctctggcccg atgcggttgc ccgccggaat
     1621 acgcacgcct tcaaaagtga tggtgcgggt cggctgggcg cgccagccca ttttcaattc
     1681 gttgcgcccg tacttcacgc cctcggcatc ggccggcacc aggaagcagg acacgccctt
     1741 ggcgccgctg tcctcaccgg tgcgcgccat cacgatcagc acgtcggtgc tgcccgcgcc
     1801 ggagataaag cacttgctgc cgtccagcac gtagtcgtcg ccatcgcgct tggcgcgggt
     1861 acgcaggtgc gcggcgtcgg aaccggcgtc cggttcggtc aggcaatagg aagccagcag
     1921 ctcaccgctg accaggcgcg gcagccaggc atctttcagc gcctggtcgg cgaaggaggc
     1981 gagcatccac gaggccatat tgtgaatggt cagaaacgcc gtggtggcga tacagccggc
     2041 cgacagctgt tcgaaaatca gcgaggcgga caggcgtgag aggccaagcc cgccatcatc
     2101 ttccttgata tacagcgcca ggtagccctg ctcggcggcg cgcttgatca catccaccgg
     2161 gaagtggtgg tcgcggtccc aatcggcggc atgcggggcc agttcacggc tggcaaactg
     2221 gcgggcgctg tctaccagca ggcgttgttc atcgctgagt tcgaaatcca taagtcctca
     2281 ttgcagggtc aggggttgcc cggcggcgcg tcgttcggcc tgccattcgg cgaggctggg
     2341 tgccgcttgg gtggcgtcga ggcgatcgac gatttcaaag tagtcttgct tgagcgggtc
     2401 cttgagtacc tcggcgcgtt ctttgacttt gacggcatac accgtctgca ggttctggtg
     2461 gtcgagggcg cgccaggttt gttgattttt gagcagctga tagctgtggc cttccagagc
     2521 cttgatcagc gcttcgctgt ccaggctgtt tgcccgtttg gcggcgtcgg cccattgata
     2581 gacgatgctg taggccgacg cggcagagct ggacggatac atctcgtagc gctggctgaa
     2641 atccttgacg aaggcttcac cgcgcacgga tttttccagc gccggtacgc gccaggtcca
     2701 gggttcggtg ccgatcacac cttccatcaa accggggccg ccttgctcga ccatgctctg
     2761 ggtcaggttg ggcgccacga tttgcatgcg ctcggtcagg cccagatcct tggcgatgcg
     2821 catagcgcgc accaggtctt cgccgaacag caccagggcc agcacctcgg cattgctcgc
     2881 ggcggcctga gtcagggcat cctgataatc ggccaggcgg gcgccgggga aggtcacctt
     2941 gaggcccgga tgctgaccgc tgtcttcgct gttggtggtc tggcgcaacg agctttccgt
     3001 agtgtggccc caggtgtagt cggaggtgat gtagaagtag cgcttgttcg gcaggttctt
     3061 gctcaggtat tggcccagca cccgcgcgct catccaggcg ttgttgcact cgcggaacat
     3121 atagcggtgg ccgtccttgc cggtggtgtc gttggagtag gtgagggtgc cgaaatacag
     3181 cagtccgtgc tgcgccgcgc gcttgcctga ggcgatggcg accgcgctgg atgagccgcc
     3241 aaacagcatg gccgcgcctt cgctagccag cttgtcgacg ttcttcaccg ctttggccgg
     3301 acgcgatgcg gtgtccttgc tggacagacg cagcggacgt ccgagcacac cgccctgggc
     3361 gttgagctcg tcgatggcca gcaaggctcc gcgcatctgt gccaatcctt cttccttata
     3421 cgggccggtg cgtgggtagt tgaggccgag ggtaatcggc tcagccgcct gtgcacaggc
     3481 agtaagcccg gcccataggg ccagggcggt ggtcaatcgt tgcatggcag ttctccttgt
     3541 tcttgttgtt ggaactcgga gaacttttta cgccattgga ctaattgttg agatcgtcta
     3601 ttggtctaac aaggccgaca ccgttctcat ttcaactgaa tggtcatatt tggtccggcc
     3661 ggcgatccgg cggctacgga gtcttcatcg aaccagcggc tggtgaccgt cttggtttcg
     3721 gtgaagaagc gcacgccctg tttgccgtag gcgtgcagat cgccgtagaa cgaacccttc
     3781 cagccggtaa aagagaagaa cggcagcggt accggcaccg gtacgttgat gcctacctga
     3841 ccgacttcca ccgcgtgctg ataatgccgc gccgcgccgc cggagcgggt gaagatcgag
     3901 gtgccgttgc cgtaggggct ggcgttgacc agttcgatgg cttcttccag ggtgtcgacc
     3961 tccatgcaca ccagcaccgg gccgaagatt tcctcgcggt acaggctcat cttggtcgtc
     4021 acgccacgaa acagcgtggc gccaatccag ttgccgttgg ggtagccctc gaccgcgcag
     4081 tgcgaaccgt ccagcaggca ttcggcgcct tccgctttac cttcggcgat caggcgcagc
     4141 acgcgctgtt tggcctgctg gctgatcagc gggccaaagg cggcgtggcc atcggtccag
     4201 taaccgggtt gcaggtcggc catctgcgcc tgcagctcgt cgatccattg cttcgattcg
     4261 ccgacaaaca ccgccacgct gatcgccatg caacgctgtc cggcagcgcc gcaactggcg
     4321 ccgaccaggt tgctcagcac ctgctgcttg ttggcgtcgg gcatgatcac catatggttc
     4381 ttcgccccgg caaaagcctg cacgcgcttc aggtgagcgg tgccggtacg gtagatatgc
     4441 tggccaaccg gcaccgaacc gacgaaggaa atggcgcgga tatccgggtg ggtcagcagg
     4501 ctgtcgacca cctcgcgggc accgtgcagc acctgcaata cgcccttggg cgcgccggct
     4561 tcgataaaca gctcggcgag gcgattgggc gtcagtgggt cctgttcgga cggcttgaga
     4621 ataaaggtat tgccggcggc gatggcaagg ggaaacatcc acagcgggat cattgccggg
     4681 aagttgaacg gggtgacgcc gacgcatacg cccagcggct gaatccagct ggcggtgtcg
     4741 atatcgcgag cgacgttctc catggtctcg cccatcatca ggctggcgat attgcaggcc
     4801 tgctcgacca cctcgatgcc gcgccagaca tcgcccttgg catcggccag ggtcttaccg
     4861 gtttcgccgg caagaatttc cgccagctcg tcgtggtgtt ccttaagcag atgctggtaa
     4921 cgcagcatca cccgtgcgcg gtcggagacg ggcacttcgc gccaagtgag gaaggctgcc
     4981 ttggcactgg caatcgccgc ttcgatctcc tcggcggtgg ccttgggtgc cagcgccaga
     5041 acttcctggg tcgccgggtc ggtgacttcg ataaattcac gggcgcggct ttcgcgccat
     5101 tcaccgtcga tcaactgcgc gattgccttg gccattgttt tctccacgcg ggacctgagg
     5161 tccgattgtt gttttgtcag tgttatagct gccttgctcg gctttgtgga ttgacgatct
     5221 tggcctgcag atggactatc ttgggattta atctgtagcc cggatgaaat ccgggaccct
     5281 ttctcccgga ttgcatccgg gctacttgag taccccaatg cgcgcttttg tcgatacttc
     5341 caactggcct ttgccggcca atggcgtgcg ctttatcacg ccacccaggc tgcgccgcct
     5401 gctggcaaag catcccctgg ggcagggctg ttatcccctg gccctggggt actatcccga
     5461 ggcgcagggg caccgcatgc atcgcccgca gccggaggat cacctgctca tctactgccg
     5521 cgcaggacag ggttggctgg agacagcgga tggacgatta gctgtcagtg gtggcgatct
     5581 gctgctgttg ccaaaaggcg tgatgcacgc ctacggcgcg gacgtggaca agccctggac
     5641 gctgtactgg gtgcattttg acggcgagct ggtggcggac tttctcaagc ccctgggcaa
     5701 ggggccgatg tggcgcatcg gcgtgcagcc acgcctgctg gccgagttcg atgccttgct
     5761 taacctacgc aaacagggtt tgaacctggc gcatttcgtt catgccgcgc accagttgca
     5821 ggtgctgctg acttctctgg cggtgctgcc ggcgcgcacc accttgaaat ccggccgagt
     5881 gctggatgtg gacgcggtgc aggcggtgat gcgcgcccat ctgcatgact cgctaaacct
     5941 cgacgagctg gccgcgcagt tcaaactgtc gcgctttcac ttcgccaaaa cctaccgcgc
     6001 cctgaccggc catgcgccga ttcaggactt tatccaactg aaaatggccc acgcctgccg
     6061 cctgctcgac gagggcgaac agggcatccg ccaggtggcc gagcagctgg gctatgacga
     6121 tgtgtattac ttctcgcggc tgtttcgcaa agtggtgggt atggcgccca gtcattaccg
     6181 ggcgctgcat caggggtgag gacggaagtt ttggccaaaa gcagacattc aatcctggtg
     6241 gcagttgaga aaaatggagt catggtatgt ctacgaaacg aggggtgatt caaatggaca
     6301 ctgtgcggga tatcgaggaa cgcatgacta gcttccttca aagccaagac cacgtgacgc
     6361 ttcttgacgg tcatgttcct cagcctgatg gcgtcaagat cgccgattat ctgtttttca
     6421 acaggcaagc ggttgtcgaa ctcaaaacac tgaagattga tccaaaagat aagattttag
     6481 atggtgccaa atcgctaatg gagtctgacg gttttccgct gatttttggc gactatgacc
     6541 ttgaaaccgc gatgaagaac acgccaggag ggcaagagca tctggacaaa attttttctc
     6601 gcgcaaccag gatggttgag ggagtgctta gaaatgccaa aaaacagatt gcctcatcca
     6661 aaaggctatt gaaccttgac cccgataccc caggggtcgc gctcatactg aatgacaccg
     6721 ttgaaagcat tcctgcctct cggctcgttg acaggttcag ctcctggctc accggtgatg
     6781 gcaaagaccc gggccggttc tcggagatcg actttataat tttgatccaa actacctacc
     6841 ggctacagca aggtagtggc agaagaacgt acctgccgac ctttatcata agtaatccct
     6901 tcaatgctca tcgccatcac aaaattgagc aagaaattca gttatttctg caagcttggg
     6961 cacgctcgca gggtcacaac tttgaaagta cgagcgaaac ctctggcctc aaatttgagc
     7021 acaaccacgg tcctcggcct ggccctcggt ctttacagga attggtagag gcacagtacc
     7081 gggcacgcag gtatatgagc gaatggagcg aagagagact aatagctcat ggcaaggatg
     7141 tgattcagaa gatgctccta atatttctca tagacgcaga aaagccttca gaagccgact
     7201 ctcatcgcta cataaagcaa tttacggagc tgcttgagga aggtcggata cggggctttg
     7261 acttgaggaa agtattgaaa gagatcccta gaaccagata gttactattc gtctatcgct
     7321 ttgactcgga ggactgtagt agagcaattt gaatgcctgg tcgtcgcgcg tagcctaggc
     7381 cggcttccga tagctctttc acttgcttgt gtaaatcggc atcaaccggg cgtccttggt
     7441 atttgctggc cgctctggcc ttttcaatat cttgtgtccg cacaacccca ttgttgggcc
     7501 tcactgcgct caaccacaac ctacgcggcc acgcgccacc aaccttgact tgcccagctg
     7561 ccttccttag cgtgccgctt ccgttgagat aagcaggaca caggcatgag cgccgcagat
     7621 aacgtcaaac tcgaagccgg ctggaaggcc gctctgcatg aagagttcga gaagccctat
     7681 atgcgtgagc tgggggattt tctgcgcagc gagaaggccg caggcaagca aatttatcca
     7741 cccggttcgc tgatcttcaa tgcgctcaat tccacgccgt tggcgcagac caaggtggtg
     7801 gtgatcggtc aggaccctta tcacgggcct ggtcaggctc acggcctgtg cttctcggtg
     7861 cagcctgggg tgaagacgcc gccgtcgctg gtgaatatct ataaagagct caagcgcgac
     7921 ctgaatatcg atattcccga ccacggcaat ctgcagtatt gggcggagca gggcgtgctg
     7981 ctgcttaaca cctcgttgac ggtggagcat ggcatcgctg gctcccatgc caagatcggc
     8041 tggcagacct ttaccgacaa ggtgatcgag gtggccagcc agcagcagga gcacctggtg
     8101 tttctgctct ggggtgctca tgcgcagagc aaggaaaagc tgatcgacag cagcaagcat
     8161 ctgattctga agtcggtgca tccttcgccg ctgtcggcgc accgcggctt tatcggtaat
     8221 ggccatttca gccgtaccaa caagtttctc cagcagcatg ggctaagccc gattgactgg
     8281 cgcttgcccg aggcctagca gccagttaag cagcgtcgca ctgctgctct gccagatcgc
     8341 ggcgcaggtc gcgcaagcgg tgactcaggc gctcgcgctg agcttcatcg ctgctgctca
     8401 gcagctgact gaacagcgtg gccagcgcct ggcgtgagcg gccataattg gcgcggtatt
     8461 cgtcgttgta gaagctctcg cgttgttgca gcaggcgggt cagccgctcg gcgaagtcgg
     8521 cgctgtcgcg ttcggcgacc accttgcgca gttcggcctg ccacagttgc cgattctcca
     8581 gccacaggcg gttctgctcg ccgaggccat tggcccaggc ggcgatatgc tcaatttgtg
     8641 cattgttcag gcggccgagc caggaccgca ggcgttcctg catgcgcgtc tggcgttcgc
     8701 tgatctgggt ggccaggggc gggtcgagaa agtcctgacg atcttccaga ttgtcttcat
     8761 caatcgcggc gtaaagctcg ttgacctgct gttcgctcag accctgcagc aattcgatgg
     8821 ctgttggggt gatctcgacg ctgatgcgtt tgagcgcggc atcgaattgg gcgaactgct
     8881 caagcagctg agcgctgtct ggctgcggct gggcgaggat gctctcggtg gtttgcagcc
     8941 agtcgatata acgcggcagc tcggcgctgc agtgccattg caggtgcgtc tgcaagcggg
     9001 gtttgagcca ggcctgctgc tggctgttga ggttcaggta gtcgttgagc cgccagggca
     9061 ccagccagtc caggttgcga tacgctaggc cgacgcggct gcaggcgcta atcagaaagc
     9121 tcaggcagag caatagcagc agggttttca cggcgggcca acgcagtacg ctcatggagg
     9181 atgcctcggt ggtgaaggcc tgagggtacg ccagacgtgc gcctgtgcgt ggctgcagac
     9241 atatctgtta aacaaaaact gctgcgttgc aggcaaaaaa gagcccgcac aaggcgggct
     9301 aaacgtggtc gtcaaagcga tttcagctgc gttgccaaag cctgaacaac ggctcggcga
     9361 gaaacatcac caaaaacaat cgcagcacct gcaaggcggt aaccagcgcc accgagaggt
     9421 gcagggcttc ggcagtcagg cacagttcgg tgatgccgcc gggcatcatg ccgagcatca
     9481 gcgcattctg gttttccccg gccagccaac ctagcccttg gcctaacgcg gcggcggcga
     9541 gcatggcgag caggcagaac agcaggatgc gcgcgagaaa acccggcgcg ctgcgaaaga
     9601 agttacggtc gaagtggcaa cccagggcgc agccgatcag ccactggccg acttgtccca
     9661 ggccatcggg cagacccagg tgcagatcga agctgacact ggccaccgcg cagacggtca
     9721 gtgggcctag catccacggg ttgggttggc ccaaacggcc ccacaacaag gccagcagcg
     9781 cgccgccggg gagcagcaca gccagccaca accaactgac cgctgcaggc gcgggcggct
     9841 cgataggcgg caggctccag gtgaagatcg ctggaatcag cagcaccacc aacagcaaac
     9901 gcaggctgtg agcagccgcg accttggcgg tttcggcgtt gtgccgttgt gccaggttga
     9961 ccatctcgct ggcgccaccg ggcatgctgg caaagaacgc ggtggcgcga tccaggccgg
    10021 cgcggcgcag cacgataatg ccgatcaggc ttagcagtaa ggtgagcagg gcgccaagca
    10081 gaatgacgcc gaagtggcta aggagttggc tcagcacttc actggtgaaa tgcaggccga
    10141 tggcgctggc caccagccac tggccggtct tgcgtccacc cggcaactcg gtgatcagcc
    10201 agccgctgca acgtgcggca ataaccgcca gtagcgagcc gatgatccac ggcaaaggcc
    10261 agccgatcag gctggccaga tagccgccta acaagccgac caggggggtg gcccaccagc
    10321 tttgccagcc ggtgagcgca aacaacttag gcattggcgg cattggccag ttgcgccgca
    10381 cggcgcgaac gccaccagcg caggcctggc agggccagca tcaacgcagt gagtgcccac
    10441 aggcacaggc tgattgggct gttccagagg atgctcaggt cgccagcgga tatcgacagg
    10501 gcgcggcgca ggttgtcttc catcagctcg ccgaggacga agccgaggat cagtgccgac
    10561 agcgggaagt ccagcttgcg taggatatag ccgaatacgc cgaggccgat catcagcacc
    10621 aggtcaaagg tggtgctgtg caccgcgtag acgccgacca tgctgatcac ggtgatcgcc
    10681 ggcaccagca cccagttggg tacgctgagc atgcgggcga acaggccaac cagtggaatg
    10741 ttcatcacca gcagaatcac gttgccgatg aacaatgagg cgatcaggcc ccagaccacg
    10801 tccggttgct gttcgaacag cagcggaccg ggggtgatgt tgtacagggt cagcgcgccg
    10861 atcatcactg cggtggtgcc ggagcccggt acgccaaggg tcagcatggg gatcagcgag
    10921 ccgcaggcgg aggcgttgtt cgccgcttcc ggcgccgcca gaccgcgtag gtcgccgtcg
    10981 ccgaacttac cgttcgggcc ggccatgcgc ttttcagtca tgtaggtcat ggcgctggcg
    11041 atggtcgcac cggcacccgg cagtacgccg atgacgaagc cggccacagc gctgcgcacc
    11101 atggtccaga aggtgaagca gaactctttg aagttgaaca gcaggcggcc gctggctttc
    11161 actgctttct ggccgttgtg ggttttttcc agcatcagca gtacttcact gacgctgaag
    11221 aagccgatca ccacgatgac gaactggatg ccgtccgaca ggctgacgct gccgaaggtg
    11281 aagcgataca cgccggtggt cgagtccacg ccaacggtgg ccagggccag gccgatcagg
    11341 gcggccatca gggttttaac cggtttgtca ccgaccatgc cgccgaggca ggcgatggcg
    11401 aagatcatca acacaaaata ttcagccggg ccaaaggcca ccgcccattt cgccagcagc
    11461 ggggcgaaca gcaccacgcc gcaggtggcg atcatgctgc cgataaacga gctgaccgcc
    11521 gacagcgaca gggcaatgcc ggccttgccc tggcgggcga gtgggtagcc gtcgagggtg
    11581 gtcatcaccg ccgcggcatc acccggtacg ttgagtagaa ttgccgagat acgcccgccg
    11641 tattcacagc ccaggtacac ggcggcgagc agaatcagtg cggtttccgg cgacaggccg
    11701 agggcgaagg ccagcggcag cagcagcgcc acgccattga ttggcccgag gccaggcaac
    11761 aggccgacga tggtgccgac aaaggcaccg aacaaggcca ccagcaggtt ttctggtcgc
    11821 gtggcgactt cgaagccttg catcaagaaa ttcagggttt ccattttcag ctctccacga
    11881 aggcttcgaa caggccgagg ggcagcggca catccagcag gtaatcaaac aggccataca
    11941 gcagcacgcc catcagcacg gcgctgatgg cgcaaggcag cgggcgaccg gtaaacagca
    12001 ggccaagggc gaaggtggcg agcgtggtgc tgataacgaa gccgagcact tcgaacagca
    12061 gcgcataggc cagcagcgcc aacacgcaga gcacggcgcg cacggcggcg cgggtattca
    12121 gcgtctgctc cagcaggccg ggcttgaaca ccagccacag gctgccagcg gccatcaggc
    12181 tgagcagcag taacggatag gcgcgcggcc cgaccgggtc ataagcgaag ggtgcctgga
    12241 agccccaggc gatgacggcg agaacggcgc aaaacagcag ccagctcgcg gcgaacaggc
    12301 gaacgtacat ggcggattac ctcagggctt gtgcgtctaa tcccctctcc cttgggggag
    12361 agggttaggg tgaggggctt ggatggtgcg gtctggaacc gccacccctc accccgaccc
    12421 tctccccagt ggggagaggg tggcttacgc gttactgcat caggccgaac tcgccagcca
    12481 aggccttgta ttcctcaact tgcttgaaga ccaaagcttt cagttcgtcg ccagtcaccg
    12541 acaacggcag cagatcgcgc tgttcacgta gggtggcgaa gtcttcacgg gcaagcaggg
    12601 tgtcgaactg ctgtttccac cagttgtact gctcgtccgt gacctctggg cccatataga
    12661 agccacggat taccggccag acgatgtcgt agccctgttc cttggcggta gggatatcgg
    12721 ccagtttgcc tggcagacgc tcatcggaga gcacggcgag gatgcgcacc ttcttcgccg
    12781 ccagctgcgg ggtgacttca cccagaccgc tggaggtgac ctgcacatgg ccgccaagca
    12841 tggcggtgag ggtttcgccg ccgccttcaa aggccacgta acgcaggttt ttcgggtcga
    12901 taccggccag acgggcgatc agcgcggtct gcatccagtc ctggccgccg atggtggcgc
    12961 cagcgccgaa gacgatgctg gtcgggtctt ttttcagggc ctgcaccaag tcatcgaggc
    13021 tctgatacgg cgagtcggca cgcacggtga tggcgccgta atcggtgccc accgctgcca
    13081 gccagcgcac gccggtttcg tcgtaacggc cgaacttgcc ttgcgccagg ttgagcagtg
    13141 agccgctgga gaacgcggtg atggtgcctg gatcatcggc acgctgggcg attactgcgt
    13201 tgtaggccac cgcgccgacg ccgccgggca tgtaggtgac gcgcatcggt gctttcagca
    13261 ggccgctgtc tttcaggccg ctttgcgcca gtttgcaggt caggtcgaaa ccgccaccgg
    13321 gcttggccgg ggcgatgcat tccgggcgct tgggttcggc agccagtagc tggccggcaa
    13381 aggccataca ggcggtggcc agggcgatgc gggtaaaggc agttttcatg gcgtgttcct
    13441 ctgaactctt attgtggttt accagatcgg cagtgtgtag ccgacgatga cgcggttctc
    13501 gtccgcatcg cgggcgaagt cggatttgaa gctggcgttg cgcaggcgca cgtagacatc
    13561 tttcagtggc ccgctttgca ccacgtactt gacctcgata tcgcgctccc actcgccgcc
    13621 ggtgtcatca gtgccggcaa tctgcgcgtc gctgcctttg atgtagcggg tcataaagct
    13681 caggccgggt acgccgagct tgccgaagtc gtagtcgtag cgggcttgcc aggagcgctc
    13741 gtcggcgttg gcgaagtcgt tgatctgcac gaagttgacc aggaacgggt cgctgccatc
    13801 gatataggcg taaccggtgt cgccgctcat gtcctgaaag cccaggccga gtttgtggcc
    13861 gtcgatcgcg tagctgagca tggcgttgaa ggcgcggttg tcgatcttgc cggcattgga
    13921 tgcgccgctg tcgtcgctca gcatcatccg tacatcggcg ctcagggtgc cgctgcccag
    13981 cggttggctg gcgagcaggc cgaagaagtg ctggcgatag atatcgtcga ggtcggcgaa
    14041 gtagtagcgg cctttgagct gcttgttgaa gctgtagtcg aagcccatca gatcgagatg
    14101 gtcggcggta atgccagcgg caaagcggcg gttcttgctg ttgaggatca gctcctcgga
    14161 gttggtcgag gcgcggtcga ttactttgtc caggcgacca ccggtaaagg tcaggccgtc
    14221 gatttcgctg gaggtcagca ggccgccttc gaatacctgc ggcagcaggc ggctgtcgct
    14281 ggccttgacc accggcagct cagggatgtg cgaaccatag cgcagctcgg tattggagac
    14341 cttgacctta gcggtcaggc ccaggcggcc aaactgatcc ggagtgccgc catcgtcctg
    14401 taccggtagc aggtcggtgc cggtactgcc gccgccggaa tccagcttga cgccaaccac
    14461 gcccagggca tcaaggccga aaccaacggt gccttcggtg tagccagact ggacgtcgag
    14521 ccagaaaccc tggccccact cgtcacgctt gttctgctgc gagcttgtgc cttcgcggaa
    14581 atcacgattg aggtagatat tggtggtggt caggctggct ttgctgtcgg cgacaaaatc
    14641 ggcgtgggca gcgggggcga gcagggccgt gctgatggcc aggctaagca ggtgtacagg
    14701 cgaaagtgcc tgatgagatg cagtcggcat cttgtggtct ccattgttgt ttttgtcacg
    14761 gctgcgcacc actgcgtcag tcgttcaggt cggtggatcg agccacctga aaccgatgct
    14821 atggggtcaa gctttcacca ggctttcagc ttgaaagaaa gttgctgggg cgttcgcagc
    14881 agcctttgcc ccgttacact gggcgcacaa gaacaatgtt gtggagtgcc attaccgtgc
    14941 gaatcctact ggtcgaggac catctgcccc tggccgaaag cgtggcccag gccatgcgtg
    15001 gcaccgggct aaccgtggac atgctgcatg acggcgtggc tgccgatttg gccttaagca
    15061 gcgaggatta cgccctggcg attctcgata tcggcctgcc gcgcctggac ggatttcaag
    15121 tgctggcgcg tttacgtgag cgtggcaata ccttgcctgt gctgatgctc accgcccgcg
    15181 gtgaggtcaa ggaccgcgtg catggtctta acctgggtgc cgacgactac ctggccaaac
    15241 cctttgaact gagcgagctg gaggcacggg tcaaggcgct gctgcgccgc accgtgctcg
    15301 ggggcgaacg gcagcagcgc tgcggcaacc tgctgtatga cctggatacc cgacgcttta
    15361 gcctcgccga tgccctgctg gccttgactt cccgcgaaca ggcggtgctg gaagcaatga
    15421 tcgccaggcc cggcagggtg atgagcaaag agcaactggc cgcccaggtg ttcggcctgg
    15481 atgaagaggc cagcagcgac gccatcgaaa tctacgtgca ccgcttgcgc aagaagcttg
    15541 agggcggcgg ggtacgcatc gtcaccttcc gtggtctggg ctatttgctg gaggctgtgg
    15601 gtgactgatc atcggccgct tacgatgcac gagccgggta gcctgcgcgg gcggctgatt
    15661 cgacgccttg ccttgctgct ggcgctgatt ctgctggcca gtagcctgag cgcctactgg
    15721 agtgcgcggc gtgccgccga caccgcctat gaccgcaccc tgttggcctc ggcgcgggcg
    15781 attgccgatg gtctgtacac cacggcggat cgtctaagcg ccaacgtgcc ctatgtcgcg
    15841 ctggatacct tcgcctatga cagcgccggg cgtatctact ttcaggtgct ggatgtgcag
    15901 ggcgcactgg tgtccggtta tgaagacctg ccgccggcgc cgccaggcac caagcgcacc
    15961 gaggattacc cggcgcttgc gcatttctac gatgccgaat accgcaacca gggcgtgcgg
    16021 gtggtcagct tgctacagcc ggtcagcgac ccgggggtca atggtgtggc ggaaattcgc
    16081 gtggcggaaa cccagggcgc gcgcgagcgc atggcacgcg agttgttgct tggcactttg
    16141 ttgcgcatgg gcgtgctgtc gctgagtgcc ttgctgctgg tgtggttggc ggtgagcgct
    16201 gcgttgcgtc cgctggaagc cttgcgccgc agtgtggcgg cgcgtaccag cgacgatctg
    16261 cagcctctgt cggatgttgg catgccgcgt gagctgcgtc cgctgatcga tgcgttgaac
    16321 cagtttaatg accggctgcg cagtctgttc gaacgccagt cgcaatttat cgccgatgcc
    16381 tcccatgagc tacgcacgcc cctggcagcg ctcaaggcgc gggtcgagct gggcttgcgc
    16441 gcgcaggagc cgacaatctg gcgtagcacg ctggaggaag cggcgcagaa caccgacaaa
    16501 ctcactcatc tggccaatca gctgctttcg ctggcgcgta tcgaaagcgg cgcgcgggcg
    16561 attgccgaag gcggcgcgca acgcctggat ctcagtcaac tggcgcgcga attgggcatg
    16621 gccctggcgc cattggccca tgcgcggggc atttccctgg cgctggaggc cgagcagcca
    16681 gtgtggctca gcggtgagcc gaccttgctc aacgaactgc tgtgcaacct gctggacaat
    16741 gccctagcgc acaccgcagc gggcggcaat gtgattctgc gggtattgcc gaccggggtg
    16801 ctggaagtcg aggacgatgg cccggggatt ccgccagaag agcatgagcg ggtgtttgcg
    16861 cgcttctatc ggcgcagcaa caagggctcg ggtgcgggct tggggttggc gattgtcggc
    16921 gaaatctgcc gggcgcatca ggccgagatc agcctgcatc aggcgcagcc gcaaggtttg
    16981 ctggtgcgcg tcgcattcgc cagtgctgaa gcttgaatca gggcgccagg ctaagcggct
    17041 gctgaatttc caataggtac tgggcgatct tgccgctttc acgcagttgc gccagaccct
    17101 ggttaaagcg ggcgatcagc tcggcattgc cgggcacttg ccgcgagagc agcaggtgca
    17161 ggctgtcgct gcgcagcggt ttggggtgaa agctcagctg agcgcggtcg gcggcactga
    17221 agtgctgatg cagcatgtcg aagccgacca ccttgtccat cgggaacagg tcgacacggc
    17281 cggcaagcag ttggcgaaag gcggtttcat cgctggtgac ccggttgacc cgcaactgcc
    17341 cgctggcttc ggcctgctga aagtcttcgc cgtagtcata gccgcgggtg ccaactaggc
    17401 gccggccttg caggtcgacc acctgcttcc agtcaaagct gaaacccttg cggtggaaca
    17461 ggtagtaacc gctttccact actgggtcgc tgataaagaa cttttcctca cgcttgctat
    17521 tgtgcaacca gactgcgctg ccgtcgcgtt tgccgcgctc ggccagttgc agcgaacgtg
    17581 cccaggggtg gaactcccag gccacgtcga taccctgcag ggcaaaggct tcggcgacga
    17641 tgcgtgaggc gacgcccttg tgcggcagct gttcacccag atagggcggc cattcaccat
    17701 tggtcaggcg cactgagtcg gctgcagcga gaccaccgca gaacaggctg aacagaacgg
    17761 ataacagcag ggtgcgcatg aaccgtatgc cgggttgggg gactgtttcc agtctagttg
    17821 cattcggctg catgcgcgtg aagggctcta ctggagcatg ctcatggccg cttccatggc
    17881 ggcggagagg tcttcgtctt cgttcaggtc ctggcttggg tcaaggccaa gcttggcgaa
    17941 ggctgggatc tggctccagt ccagtttggt gtaggggtgc tcgctgccga tgtagctctg
    18001 cagggtggcg acctggacta tatcgacgta gtcgaccttg gcgctgtcgc gggtgaagtc
    18061 caggtactgg ccgggcacca tggcgatcag ctcggggaat tcccaggtgc gcaggatctt
    18121 gtcgccgatg attgggtgga tctgctcaat cacgtggttg aggctgatcg agtcggccag
    18181 tagctcgctg tgatcttcgg cgtaggtgag gatcggcagc acgccgatct gatgcaccag
    18241 gccggcgagg gtggcctggt cgggcagcag gcgggtgtag tgtttgcaca gcacgtggca
    18301 gataccggcg atttccgtgc ttttgttcca cacttcgcgc attttgcggt cgactacgtc
    18361 gctggtggcc tggaacatct gctccattgc caggcccgtg gccaggttgc aggtgtagtt
    18421 gatgccgagg cgaccaacgg ccatctgcag gtcggtgatt tccttgttgg tgcgcagcag
    18481 cgggctgttg accactttga tgatgcgcgc cgtcagggcc gcatcgttgc caatcacctt
    18541 gctcagttgc gcgataccaa tgtccggatc ctctgccgcc tcgcggacct tcagcgcgac
    18601 ctccggtaag gtcggcagca ccagttcatc gttgtcgatg gcctggatca gttccttttg
    18661 gactttctcg gcaagcttgc tcatgggcaa ttctctttag acggtcagca gtagtaataa
    18721 gtgtagtaag cgcttagcgc tgtatttcgc ggtctgagtc cagtgtatac ggcaaatcga
    18781 gcacgctcag cgtgctgcct tcggcagaac ccaggtgaat tacgccattc agcgcggctt
    18841 cgtcctgcag cacggcaagc agttcgacgc cggccgggct gcgtgcggcc agcactactt
    18901 caccaacgct ggaggtgtgc gtcggcgaga acagcgccgt ggcgggttct ggcacctggc
    18961 tgtcatgcag ctcgaggcgg tataggcgac gcttgagctt gcccaggtac tgcatgcgcg
    19021 cgacgatttc ctggccggtg tagcagcctt tcttgaaact cacaccgccg acggcttgca
    19081 ggttgatcat ctgcggaata aacagctcac gagtgctgcc catgacctgg ccgataccgg
    19141 cgcgtacttg tgccagtagc cagtcattga gcggcacttc tggcagttgg ctggccaatt
    19201 gtgcctgtac gactgcggcc tggctggcgg gtatccagag ttcggcgcgg ccatcgctca
    19261 ggcgcagtgc cagcaactca ttgctgcgag cgacgctgtc agtggtctcg gcaagctcca
    19321 gaccgaggct gcgcagggca tcatcgccgc cgctcaggcc gatgcgcagc cagtcctggc
    19381 tttcatcggt gagtttggat ttgctgaaga cggcatattt cttcaggtcg gtcagttgtg
    19441 cttccagcaa atcgctggcc attgccatca tgtagccgtc gctcacactg acaatgcgaa
    19501 agctcgactg catgcggcct ttgggtgtgc agcgcgcacc caggctggag aggctgtcgc
    19561 tgaggtaatt gaggttgcag gtgacttggc cctggaggaa cttgttggcg tcagggccgc
    19621 gcacggcgag gatgccttcg tggctgaggg tgcaatagaa cgcggtatcg gccatggtgg
    19681 attaggtatc gctggataaa agttaggggg ctcatcatag agcgcgcctc gcgccttgtc
    19741 agcgcttgcc gccgcttcgc ctgcggacat tacctggtcg gctgatgcgc gcggtcaaca
    19801 ggggcgcttt tcatcggtat actgcgcgcc ttatccaagg agcataccca tggttgactc
    19861 ggttgaactc aatcgtcttt tttggcacag ccgccgcggc atgctggagc tggatgtgct
    19921 gctggtgccg tttgtcaggg aggtgtaccc acacctcgat gaggctgacc gtgagcgtta
    19981 tcgcaagctg ctcgagtgtg aagatcagga catgttcggc tggtttatgc agcgtggcga
    20041 gccggaagat gccgatctca agcgcatggt tcggatgatt ctggaccgtg tccagcccca
    20101 gtgatgcctt cgaaagccag tggcagcctt cacggcagct gctggtttgc tatgccctga
    20161 ttcaggggct ggcgctgctt agcttggtgc tggtggatat tccgctgtgg gcacgtttgc
    20221 tcggcattgg cttctgtctc gctcatgccg cttgggtgct gccacggcat ctgctgttgg
    20281 gttcgtcttc tgcctaccgg gctctgcgct tcacggccga aggttggcag gtgcacagcg
    20341 cggcgcaggg ctggcaggct attgagttgc accccgatag cctggccttg ccgttgattg
    20401 tgctgttgcg ctttcgtcta gccgggcaac gtcgtgtgcg ggctttgtgt atcgcccgcg
    20461 acagcctgcc ccgtgatcag caccgccgcc tacgggtgcg gctgaaattc agtcgccata
    20521 ggtgggcggc agcagaatag tgtccttggc ttcggccaac tgctgcgggt agtccagggt
    20581 gtagtgcagg ccgcggcttt cgtggcgcag catggccgag cgaatcatta gctctgcgac
    20641 ctgggcgagg ttgcgcagct cgatcaggtc gcggctgacc ttgtagttac tgtagaactc
    20701 gtgaatttcg tcgagcagca ggcgcacccg gtgttcggcg cgctgcaggc gcttgttagt
    20761 acgcacgatg ccgacgtagt cccacataaa acgccgcagt tcgtcccagt tgtgcgcaat
    20821 gatcacgtcc tcatctgaat cggtcacctg gctggcgtcc cagccgggca ggtcgcttgg
    20881 catgctgatc tggtcgagct gagcgaggat gttctcggcc gctgagcggg catagacaaa
    20941 gcactccagc agcgaattgc tggccatgcg gttggctcca tgcaggccgg taaaactggt
    21001 ttcgccgatg gcatacaggc cggggatatc ggtgcggccg ctctggtcga ccagtacgcc
    21061 gccgcaggtg taatgcgccg ccggcactac cgggatgggc tctttggtga tgtcgatgcc
    21121 gaagtccagg cagcgctcat agacagtggg gaagtgcgat ttcacaaaat cagccggttt
    21181 atggctgata tcgaggaaca cgcagtcgat ccccaggcgc ttcatctcat ggtcgatggc
    21241 gcgggcgacg atatcccgtg gcgccagttc ggcgcgttca tcgaagcgcg gcataaaacg
    21301 ctcgccgttc ggtagcttga gtagcgcacc ttcaccgcgc agtgcttcag tgaccagaaa
    21361 actcttggct ttcgggtgat acaggcaggt cgggtggaac tggttgaatt ccaggttgcc
    21421 gacccgacag ccggcgcgcc aggccatggc gataccgtca ccgcaggcgc cgtccgggtt
    21481 gctggtgtag agataaacct tggctgcgcc gccggtggcc agaatcacaa agcgcgcgct
    21541 gaaggtgtcg acctcgccgg tgctgcgatc caggacatag gcgcccaggc agcgttgtcc
    21601 atccaggccg agtttgcgct cggtgatcag gtcgacggcg acgcgctgct ccagcagttc
    21661 gatattgctg cgctgtcggg tctgttccag cagggtattg aagattgccg cgccggtagc
    21721 gtcggccgcg tgaatgatgc gtcggtggct gtggccgcct tcacgggtca ggtggaattc
    21781 gaaaccgccg tcttcgcgag cgtgttcatc gtcgcgggta aagggcacgc cctggtcgat
    21841 cagccactgg atggcttcgc ggctgtgctc gacggtgaag cgcacggcgt cctcgcggca
    21901 caggcctccg ccggcattga gcgtatcctc gacatgcgat tcaatcgtgt cggtatcatc
    21961 cagtacggcc gccacgccgc cttgtgccca ataggttgag ccgttggaca ggttgccctt
    22021 gctcagtacg gcaatacgca ggtgctccgg cagcgtgagg gccagggtca ggcctgctgc
    22081 accgctaccg ataaccagaa cgtcgtgttg ataatgttgg ctcatgtccg aattccgcga
    22141 aaggcggcct ctagtatata gagggggtgg acggcacaat agccggctta tgaccaagtg
    22201 cgttactcgg gaacttttta agcgtttcgg gttccatagg cggttgtata aatggaaatt
    22261 cgcctgcgtc aggcgacttc cagtgggcct gcggtttttg cctagccggc aggtgatcca
    22321 gactacaagc gcagcaatgg agttcctttg ctgcgctgtt ccgtgcagga ttttaagtgt
    22381 tctgcaggaa gcttgtttga gggagagaac ttttgcgtaa taccccagtc tactttcgca
    22441 gaccgagtaa agggttggtg ttgcgctcct tcaagcctaa tgaggagttt tcatgctaac
    22501 ccaggaagat gatcagcaac tggtcgagcg cgtacagcgc ggcgacaagc gtgcatttga
    22561 tctattggtg cttaagtatc agcacaagat tctcgggttg atcgtgcgtt ttgtgcatga
    22621 cacccatgag gcacaggatg tggcgcagga agccttcgtc aaggcgtatc gggcgttggg
    22681 taattttcgt ggtgacagtg cgttctatac ttggctgtac cgcatcgcca tcaacacggc
    22741 gaagaaccat ctggtatcgc gtggtcggcg cccgccagac aatgatgtaa gtgccgagga
    22801 tgccgagttc tatgacggtg accatgccct caaggacatc gagtcaccag aacgcgcatt
    22861 gttgcgcgac gaaatcgagg ccaccgtaca ccgcagtatt gagcagttgc cggaagattt
    22921 acgcacagcg ctaaccttgc gtgagtttga tggtctgagc tatgaggaca ttgccagcgt
    22981 catgcaatgt ccagtcggta ccgtacgttc gcggattttc cgagcgcgtg aggccatcga
    23041 cagatccctg cagccattgc tgcaggaaac ctgagacagc ggcgctagcc aagagaggaa
    23101 ccgccatgag tcgtgaaacc ctacaggaat cgctgtccgc ggtgatggat aaccaagcgg
    23161 atgaacttga actaagacgt gtgctggcgg ccagcgatga ccctgagata cgggcaacct
    23221 ggtcgcgtta ccagattgct cgagcggtta tgcataaaga gctgcttgag ccgcgcctgg
    23281 atattgccgc tgccgtgtcc gccgctctgg ctgatgaggc tgctccggtt gcgccgcaag
    23341 tgcagcagcg cggtccttgg cgtaccgtcg ggcgcctggc tgttgctgcg tcgattaccg
    23401 tggcagtgct ggcaggtgtg cgtttgtaca accaggacga catcaccggt gcacagttgg
    23461 ctcagcaatc cactcagccg gcgctgaata ttccccaggt caacgggccg gccatgctgg
    23521 caggcttcaa tacctccgaa gagcagtccg ctcctgcagc cagtgccgta gggcaaggtc
    23581 agccgggctg gcatgaacag cgcttaccag cctatctgcg ccagcatgcg cagcaagcag
    23641 ccatgagtac gagtgaaggt gcgctgcctt acgcacgtgc agctagtctg gaaaaccgtt
    23701 aacggctcgt tgttaaggaa tcgcatgcgc tttattcctt taagcgttct gttgttgggt
    23761 agctggctgg cattgccggc agctgctgat gagggtcggg ctttgctgca gcggttggcg
    23821 gatgccgagc gcacgcagag ttttcagggc gcgttcgttt acgagcgtaa tggcagtttt
    23881 tcgacgcaca gcatctggca ccgcgttgaa gagaacggca aattacgcga acgcctactg
    23941 cagctggatg gctccgcgca ggaagtgatt cgcatcgatg gtcaggtgca gtgcgccagc
    24001 ggtgcgttgg ccgatcaagt ggtcgacagc cagctatggc ctgctcggac gctaaatgcc
    24061 gagcagctga gtgaatggta tgagcttcgc gtgctgggca agtctcgcgt ggcggggcgg
    24121 gtgaccagcg tattggcgct ggtgccgcgt gatcagcatc gctacggcat tgaactgcac
    24181 ttcgatcagc aaaccggctt accgctcaag tccttgctgg tcaatgacaa ggggcagttg
    24241 cttgagcgtt tccagtttac ccagctagaa accgtagctc ctgcagcctc caaccttgag
    24301 cctggcgcag gctgtcggcc ggtgcgtctg gctccttctc cggtggatac ggtcgaggcc
    24361 tggcgctcgg attggctgcc gccaggtttt agcctgggtt cggtcaccca gcgccgcagc
    24421 cctgcatcga atgagtctgt cgcttgtcag gtgtatgaag atggcttggc tcgtttttcc
    24481 gtctttattg agccgcttcg cgacgcggcc gttgaggatg tgcgtactca gctcggtccg
    24541 actgtggcgg tttcgcgcag gttgaagacc gatgatggtg atgtcatggt caccgtggtc
    24601 ggtgaaatac ccctgggtac ggctgaacga gtggctttat cgatgcgttc ggcagccgag
    24661 gtggtgcagt gattgaggag caggggcggg tcgttgcggt tgagcctggc gctgtctggg
    24721 tcgaaaccct gcgtaaaagc acctgctcca gttgttccgt aaaagccggt tgcggccagg
    24781 gtttgcttga tcaactcggt gcgagcggtc gtcgtggtta tgtgcgtgca ctgtcgaatc
    24841 tgcagctgaa tgttggcgat gcggtcatca tcggcgtgcg tgaggatttg ctggtgcgcg
    24901 gttcgctgct ggtttatctg ctgccattgc ttgggttatt tactgccgct gtactggctg
    24961 agcaaatggg tttgaacgag ccctgggtga tactcagtgc gctgttcggt tttctgtttg
    25021 cttgctgcgc agtgcgttgg cgcagtcgtg ttacggccgc tgatccggct ttgcaacctg
    25081 tggtgttgcg tgcattgctt ggcagtgtcg caacagcgtt ttaagacttt tgctggcctg
    25141 atggggagtt gtatgtctaa gcctagtctg aaatcttcat ttactgcgtt atttgccgtg
    25201 ttgctaatgg ggcaggcatt gtttgctcaa gcgagcctgc cggatttcac cgagctggtt
    25261 gagcaggcgt cacctgcggt tgtcaatatc agtacccggc agaagatgcc ggatcgcaca
    25321 gtcgccggca gcggtcagct caatatgcct gatctcgaag gcatgccgcc gatgttccgc
    25381 gagttctttg agcgcaatat cccgcaggtg ccgcgtgcgc ctggtggcgg tcgccagcgt
    25441 gaagcccagt cgctgggctc gggctttatc atttccgctg atggctacgt gctgaccaat
    25501 aaccatgtga ttgccgatgc cgatgaaatc atcgtgcgcc tgtcggatcg tagtgagctg
    25561 gaagccaagc tgatcggtac tgacccgcgc agcgatgtcg ctttgctcaa ggttgagggc
    25621 gctggcctgc caactgtcaa actgggtaag tcggaagagc tgaaagtcgg tgagtgggta
    25681 ttggcgattg gttcgccatt tggcttcgat cattcagtta ccgctgggat cgtcagtgcc
    25741 aaaggccgta gcctgccgaa cgagagctat gtgccgttta tccagaccga tgtagcgatc
    25801 aatccgggta actccggtgg gccgttgttc aacctggccg gcgaggtagt gggtatcaac
    25861 tcgcagattt tcacccgttc cggcggcttt atgggcctgt cgtttgctat cccgatgagt
    25921 gtggccatgg atgtggctga tcagctcaag gcggaaggca aggtcagccg cggctggctg
    25981 ggcgtagtca ttcaggaagt gaacaaggat ctggccgagt catttggcct ggataagcca
    26041 gccggtgcgc tggtagcgca ggtgctggag aacgggccgg ctgctgaagg cgggttgcag
    26101 gtaggcgatg tgattctcag cctggatggc aagccaatca tcatgtctgc cgatctgccg
    26161 catctggtcg gtgcgcttaa accaggcact actgccgaat tggatgtggt ccgcgacggt
    26221 gcgcgcaaga agctgaaatt gaacattggc gcgttgcctg aagacggtga agccttggcc
    26281 ggcaaccctg cgccaggggt tgagcgcagc agtaatcgtc ttggggtcaa ggtggttgag
    26341 ctgagtgcag agcagaagaa gagccttgat ctgaaaggtg gcgtagtgat ctccgaggtg
    26401 caggaaggtc ccgctgcact gattggcctg cgtccgggtg atgtaatcac tcacctgaat
    26461 aatcaggcga tcaattcagc caagaccttt gccgaggtgg cgcaggcgct gccgaagaat
    26521 cgttcggtgt cgatgcgcgt gctgcgccag gggcgtgcca gctttatcac cttcaaactg
    26581 gccgactaac ggcgcaggca gactaaaagg gcgacttcgg tcgccctttt acatgtcgtc
    26641 atttacatag cggcgcggcg cggccttgat gacgtgcaag gtggctttcg ggtacacttc
    26701 gcggctattt tcggcgggca gtcgcctgcg accttttcga gtgttgatcc gtgagtgatt
    26761 tgagtcatat ccgcaatttc tccatcatcg cccacattga ccacggcaag tcgaccctgg
    26821 ctgaccggtt tatccagatg tgtggcggcc tggctgatcg tgaaatggag gcccaagtgc
    26881 tggactccat ggatcttgag cgcgagcgcg ggatcaccat caaggcgcac agcgtcaccc
    26941 tgtattacaa agctaaagat ggcattacct atcagctgaa ctttattgac accccagggc
    27001 atgtcgactt cacctatgaa gtcagccgct ctctagccgc ttgtgaaggc gctttgctgg
    27061 tggtcgatgc cggtcagggt gtggaggcgc agtcggtcgc taactgctac accgccatcg
    27121 agcagggcct agaggtcatg ccggtgctta acaagatcga tttgccacag gccgatccgg
    27181 accgggtcaa agaagagatc gaaaaaatca tcggcattga tgccaccgat gctgtggctt
    27241 gcagtgccaa gaccggcctg ggggtcgatg aggtgcttga gcgtttggtg cataccattc
    27301 cagcgccaac tggtgatatc gaggcgccgc tgcaggcgct gatcatcgac tcctggttcg
    27361 acaactacct gggtgtggtc tccctggtgc gtgtgcgcca tggtcggatc aagaagggtg
    27421 acaaagtgct ggtgaagtcc accggtaagg tgcacctggt cgacagcgtt ggcgtgttca
    27481 accccaaaca cacagccaca gttgacctca aggccggcga agtgggcttt atcatcgcca
    27541 gcatcaagga cattcacggc gcgccagtgg gcgacaccct gaccctggcc gccacaccga
    27601 acgtcgaagt gctgcccggt ttcaaacgta tccagccgca ggtttatgcc ggtctgttcc
    27661 cggtcagttc cgacgatttc gaagattttc gtgatgcgtt gcagaagctg accctgaacg
    27721 attcatcatt gcagtattcg ccggaaagct cggatgcact gggcttcggt ttccgttgcg
    27781 gcttcctcgg catgctgcac atggaaatca tccaggagcg cctggagcgc gaatacgacc
    27841 tggacctgat taccaccgcg ccgagcgtaa tcttcgaagt ggtgctcaaa actggcgaga
    27901 ctatctacgt cgacaacccg tccaagctgc cggatgtttc ctcggttgag gacttccgcg
    27961 agccgatcgt gcaggccact attctggtgc cgcaggagca tttgggtaac gtcatcaccc
    28021 tgtgtatcga gaagcgtgga gtgcagcgcg atatgcagtt cctcggttcc caggttcagg
    28081 ttcgctacga cctgccgatg aacgaagtag tgctcgactt ttttgaccgc ttgaaatcca
    28141 ccagccgcgg ctatgcctcg ttggattacc attttgatcg ctatcagtcg gccaatctgg
    28201 tcaagctgga tgtgctgatc aatggcgaca aggtggatgc ccttgctctg atcgtgcata
    28261 aggacaacgc gcactataaa ggccgcgcgt tgaccgaaaa aatgaaagaa ctgattccac
    28321 ggcaaatgtt tgacgtggcc attcaggctg caattggtgg gcagatcgta gcgcggacca
    28381 cggttaaggc gctgcgcaag aacgtattgg ccaaatgcta cggtggtgac gttagccgta
    28441 aacgcaagct gctggaaaag cagaaggccg gtaaaaaacg catgaagcag gtcggtaacg
    28501 tggagattcc acaggaagcc ttcctcgctg tgctcaggtt ggatagttaa ggtcctatgt
    28561 cgatcaattt cccgctgtta ctggttattg ccgttgccgt ttgtggcgca ttggcgttgt
    28621 tcgatctgat ctttctggcc ccgcgccggc gagcggctat aagcacctac cacggccagg
    28681 tcagcgagcc tgatgaagcg gtggtggagc gcctgaacaa agagcctttg ctggtggagt
    28741 acggcaagtc gttcttcccg gtactggcca tcgtgctggt gctgcgctca tttctggttg
    28801 agccctttca gattccatcc ggttcgatga agccgacctt ggaagtgggt gattttattc
    28861 tggtcaacaa gttcgcctat ggcattcgcc tgccggtgct ggacaccaag gtgatcgaag
    28921 tgggcgatcc gcaacgtggc gatgtgatgg tgtttcgtta ccccagcgat ccgaatatca
    28981 actacatcaa acgcgtggtc ggcctggccg gtgaccgtat tgcctacagc agtgataagc
    29041 gccttaccat caatggtgag tcggtggcgc agcagctgat tggcgatgaa cccggtagcc
    29101 tgggcagtgc gacgctgtac agcgaaaaac taggtgaaac cgagcatcag atccgtaagg
    29161 aaatgcagcg ttatcgggtt gagccaggcc gcgagtgggt ggtgccgcaa gggcattact
    29221 tcatgatggg cgacaaccgc gacaattcca acgacagccg ctactggaat gaccccgcca
    29281 tccccaaacc gctgtggggt atggtgccgg accagaatat tgtcggcaaa gccttcgcca
    29341 tttggatgag ctggccagac ccgaagtcgc gcaatctgcc gaacttctcc cgcgtaggtc
    29401 tgattcactg acctgctgac gaatttgcga cgctgttaaa tgcagcgtcg caggctatat
    29461 atagtcttgc ctccagccgt aacggctttc acataactcg aatatgggga caaatatgaa
    29521 atctgcgcat tcgcaaaagg gaatgtcgat tcttagttgg ttgatggtgt tggcagtaat
    29581 cgcctttttg gccagcgcgg cgttcaaggt attgccgcat tactttgact atatgtcgct
    29641 ggagaaaatg attacctcgg ttgagaccga taaggctgct gaggtccgta gcattaacga
    29701 attctatagc catatcagca agggtatgca ggtcaacagc atccgtgatc tgaatatgca
    29761 ggacgcgatc aaagtcaaaa tcgaaaacaa cgaatttcgt gtgcacatga aatacgaaaa
    29821 acgcgagcca ttgatcgaaa acatcgatct ggtcgtgcgt ttcgataaag aattccgcgt
    29881 gcgtatgccg tgagtgcgtc gttagcccgt ctcgagcggc agctcggtta taccttcaag
    29941 gaccaggacc tgatgatcct ggccctgact catcgcagtt ttgccgggcg caataacgag
    30001 cgtctggaat tcctcggtga tgccattctc aacttcgtcg ccggcgaggc cttgtttcag
    30061 cgctttccgc tggcccgcga agggcagctc tcacgtctgc gcgcgcgcct ggtcaagggc
    30121 gaaaccctgg ccattctggc tcgcggtttt gatctgggtg agtacctgcg cctgggctcc
    30181 ggcgagctga aaagtggtgg ttttcgccgc gagtcgattc tggctgatgc tctggaagcg
    30241 ctgattggtg ctatttatct ggacaccggc atggatgcgg ctcgtgagcg ggtactggcc
    30301 tggctgacca ccgagttgga cagcttgacc ttggtcgaca ccaacaagga cccgaaaacc
    30361 cgtctgcagg agtttctgca atcgcgcggc tgtgagttgc cacgttacga agtggcggat
    30421 attcagggcg agccgcactg tcggacattc gtggtcgaat gtcagatcac cctacttaat
    30481 gataaaaccc tggggcaggg tgccagccgg cgtatagccg agcaggtggc ggccgctgcc
    30541 gcgctgatcg ccctgggtgt ggagaatggc aatgactgat tcacctgtta cacgctgtgg
    30601 ctatgtcgcc atcgtcggcc ggcccaacgt gggcaagtcg acgctgctta accacatcct
    30661 cgggcaaaag ctggcgatca cctcgcgcaa gccgcagacc acgcggcaca atatgctcgg
    30721 catcaagacc gagggcgaag tgcaggcgat ctatgttgac accccaggcc tgcacaagaa
    30781 caacgagaaa gccctcaatc gttatatgaa caagaacgcc tcgtcggcgc tgaaggatgt
    30841 cgacgtggtg atcttcgtgg tcgatcgcac ccgctggacc gatgaggacc agatggtgct
    30901 cgagcgtatt cagtacgtcg aaggtccggt gattctggcg atcaacaaga ccgatcgcat
    30961 cgaagacaag gccgagctga tgccgcatct ggagtggttg gccacccagt tgccgaaggc
    31021 cgaaatcgtg ccagtctccg cccagcatgg gcataacctc gacaccctgg aaaaattggt
    31081 cggcgagcat ttgccggaag gtgtgcactt cttcccggaa gatcaggtca ccgaccgctc
    31141 cagtcgtttc ttcgccgccg aactggtgcg cgagaagatc atgcgtcagc tgggtgccga
    31201 gttgccgtac cagatcaccg tggaaatcga ggaattcaag cgcgacgggc gcatcctgca
    31261 tatccatgcg ctgattctgg tagagcgtga cggccagaag aaaatcatca ttggcgacaa
    31321 gggtgagcgc atcaaacgta tcggccagga agcgcgcaag gatatggaag tgatgtttga
    31381 ctccaaaatc atgctcaacc tctgggttaa ggtcaaaagt ggctggtcgg atgatgagcg
    31441 cgcgctgcgc tctctgggtt acgacaatat ctgagtcgcc ctcgggcgcg ccctgcgccc
    31501 gcttttgtga gtaagcccat gcttgccctt ggccagcctg cctatgttct gcacagccgc
    31561 gcttaccgtg aaagcagcgc gttggtggac tttctcaccc cgcaaggccg cctacgtgct
    31621 gtattgcgcg gggcgcgggg caaggctggt agcctggcgc gaccgtttat tccgctggaa
    31681 gccgagtttc gtgggcgtgg cgagctgaag aacgtcggcc gccttgaccc tgctggtatc
    31741 cccaatctgc tggtgggcga ggcgctgttc agcggcctct acctcaacga gctgctgatt
    31801 cgcctgctgc ctgccgaaga cccccatccc gctgttttcg agcactacgc catgaccgtg
    31861 ctggcgctgg cccaaggccg tgctctggag ccgctgctgc gctcttttga atggcgcctg
    31921 ctcgatgagc tgggctatgg ttttgccctg gatctggaca gcgaaggtca gcccatagcc
    31981 gagacgggtc tatatcgact acagaccgaa accgggctgg tgccggtagg gcatctgcaa
    32041 ccaggtgtgt ttcaaggcgc tgaactgctg gccatggccg aggccgattg gacagcaccg
    32101 ggtgctctgg ctgccgccaa acgcctgatg cgccaggccc tggcacccca tttaggcggc
    32161 cgaccgctgg tcagccgcga actgtttatg acgctcaagg agccaagccg tgactgacgc
    32221 caatcgtatt ctgctgggtg taaacatcga ccatgtggcc accttgcgcc aggctcgtgg
    32281 cacccgttat ccggatccgg tcaaggccgc gctggatgct gaagaagccg gtgccgatgg
    32341 cattaccgtg cacctgcgtg aagaccgtcg gcatatccag gagcgcgatg tgcgcctgct
    32401 caaggaagtc atgcagacgc ggatgaactt cgagatgggc gtgaccgagg agatgctcgc
    32461 cttcgccgaa agcatccgcc ccgagcacgt ctgcctggtg ccggaaaccc gtcaggagct
    32521 gaccaccgaa ggcggcttgg atgtggccgg gcaggaagcg cggattcgtg cggcagtcga
    32581 gcgcctgagc aagatcggct gcgaagtgtc actgtttatt gacccggacc tacagcagat
    32641 cgaggcgtcc aagcgtgtcg gcgcaccggc tatcgaactg cacactggcc gctatgccga
    32701 tgcgcatacc ccagaagaag ccgcgcgcga gttggcgcgg attcgcgacg gcgttgcctg
    32761 cggcctgcag catgggctga tcgtcaatgc aggccacggc ctgcattacc acaacgttga
    32821 accggtggcg gcgattgccg ggatcaacga gctgaatatc ggccatgccc tggtggctca
    32881 cgccttgttt gtcggcttta agcaggcggt ggcggagatg aaggcattgg tagtcgcggc
    32941 gtcagggcgc ggctgattgc atcccccgtt cctacgataa gggtttacgc acgatcagca
    33001 cgccatgagc tcactcaatt gtgctgatca cggaacgccg ccagcacctg gacggccagc
    33061 gcagccgccg catcgccccg gttgctgccg cgcagcagct tgatgtgata gtcgcccagc
    33121 ggcggcaggc cgtgttcttc gcccaagcgc cttagtggtg acctgaccag gctgcgggga
    33181 aagggggcca cggccaggtc ggcgatcatg gccgattcct gcccggcaca ctgttcgcag
    33241 gaataggcga tgcggtaggg cagatcgagt cggtcgaggg cgtccagtgc ggtcctgcgc
    33301 caggcgcagc ctggatacgc cagggccagc ggcaggggcg agcgctgcac cgccaaccca
    33361 ccttcgcgcc ctgcccagac cagcgcctcg gaatagatga tttcgccgcg tgagtcgtcc
    33421 tggccgtcgt tgccagcgtt gatcaggatc aggtccagct caccggcgtc cagtttctcg
    33481 atcagatcgg cgctgcggcc gactgcgacg tccacctgca cggctggatg actgcgggcg
    33541 aacatcgaca gaaccccggg cagtacacgg ctgccgaggt cggtcgggct gccgaagccg
    33601 acgcggccgg tcagtgccgg tgcgagaaag tgggcgaccg cttcctcatt gagcttgagc
    33661 agacgccgcg cataacccag cagcatttct ccgtctgggg tcagacgcac ccgcctggct
    33721 tcgcgcacga acaggttgcg ccccagagtt tcttccaggc gtttgatctg catgctgacg
    33781 gccgccgtgg tgcgataaac ctgtgcagcg gtgcgggtaa aactgccgct ttcggcgatg
    33841 ccgacaaagg tcctgagcac atcgctttcc agtagcggct gcaacgttgg cgcggatgcg
    33901 ataggttgga tgttcatttt atgcattaat aatttcttaa cctggcgtta tgaaattgtc
    33961 gtttgtctaa acgataggcg cgattcaccc tagattcaag tcgtgtcgat acctgcatgc
    34021 agcaggttca ggtgcggctg ttatttatag ggagggtcag tcatgagcga ttcagacgat
    34081 cgtaaagtac ccgtcaaccg ccgcccctac gatttgcgcg agacggagtt ctatatgccg
    34141 gccatgtggc cgatggatct gcagaatgcc atcgtcgatt ggctgggcgc gtggcgttgg
    34201 cggcgtcaga tgcgccattt gctgcagctc gaagcccgcg ggctcgcccg cctgggcgat
    34261 gcccgggatg cggtcagcca gggtgcggca gcgcctctgc gggaaatcgc cgcgcgcaga
    34321 gcggcccgac gtcgcggtga cgacaggcaa cagtgatcag ggcttgcgtg ccaccagcac
    34381 ggcgcggcag ggggccggca ggccttcgat tgtgcggctg tgatcggccg ggtcgaggaa
    34441 ctcgggcagc gactggaagc gcatccattc ggtggcgcgt tgctcgtcca cgctggtggt
    34501 gctgacatcc acgcagcgca catcgacgaa gccggcgcgg cgcagccaca gctccagcgc
    34561 cggcactgag ggcaggaacc agacattgcg catctgcgcg tagcggtcct cgggtaccag
    34621 tacctgctgg gcatcgcctt ccaccaccag ggtttccagc accagttcac cgtccttgac
    34681 caggcagtcc ttgaggtcga tcaggtgatc gatgggcgag cggcggtgat acagcacgcc
    34741 catggagaac accgtgtcaa agccttgcag cttaccgggc agctcctcga aggccagtgg
    34801 caggtgccag gcgggcaggt cgggcaggta gtttttcatc gccagaaact ggcagaggaa
    34861 cagccagttg gggtcgacgc ccaccacgct gtccgcaccg gccccgagca tgcgccacat
    34921 gtagtagccg ttaccgcagc cgacatccag cacccgcttg cccttgaggt cgagataggg
    34981 agcgacccgc tgccatttcc agtccgagcg ccattcggtg tcgacctgta cgtcgaacag
    35041 atgaaagggc cccttgcgcc aggggatcag gccctgcaag gcggttttca gtgcggcgcg
    35101 ggtcggctcg tcgcagtggc cgttgaagct gaaactgtgc agcagctcga gctgttcgac
    35161 gttcagttcc ggtagcgcct gtaccgcgcc gtaccagcgc ggcaggtcgc catggccgac
    35221 cgccagcttg gcgtcgatct gcccaggcag gtcggccgcc cagtcctgca gtggagtgcc
    35281 ggccagttgt gcttgcaggg cgtcgaggtc gaggcggctg atcatggcaa tgcaaccagg
    35341 gaggcgaagt tgaggcactg gaaccagggc accacctggc tgaagccggc agccagcagg
    35401 cgttcgcggt gctgctccag gctgtcgggc agcatgacct tctcgatggc actgcgcttc
    35461 tgggcgattt ccagttcgct gtagccgttg gcgcgcttga aggcgatatg cagttcgccg
    35521 agcaggtcgt gttgcgcctg gtcttcgaag cgcagttttt ccgagaggat cagtgcgccg
    35581 ccgggcagca gggcctggcg gatccgtgag agcaggggca ggcgctgttc gggctggata
    35641 aattgcaggg tgaaattcag cgccaccagc gaggtcggtt gaaactccag ggcgagaata
    35701 tcggcctcga tcacctcaac cggcagcaac tcctgaaaca tcgaatcctg cgcatgcagg
    35761 tattcgcggc agcgctcgac catggcgctg gagttgtcca cggcgatcac ccagcaatct
    35821 tcgatctgca catgccggcg cagcgcctgg gtcacggcgc caagggagct gccgaggtca
    35881 tagagcacgc tatgcggctg ggcaaaccgg ccagcgagca cgccgatatt ctcgacaatg
    35941 gtcgggtagc ccggcaccga gcgtttgatc atgtcgggga aaacccgcac cacatcctcg
    36001 ttgaacacga agtccggcac ctgggccagg ggttgggcga aaagacggtc gggctgtttg
    36061 ctcacgatgg ctgcgcactg gtcgataaag ccgggcattt tagccaagtg cgcggtatag
    36121 ccaaaccttg attgccgtta ttggtcgcaa atctgcatgg gcgatggctc agcggactgt
    36181 ttgaaggccg aggccgcaat ggcccattgt cccaaccagt agctgagcat gatcgccaat
    36241 ggcgcggcct cgaagggtgc gacaacgcgg ttgatgccga tcagactgtc ggacagcaca
    36301 aacagggcgg cacctgcggc ggccaggcgt gtggagctgc ggttcaggcc gggctggtca
    36361 aggcgggcga gggcgcgcca gagcatggcg ctgatcgcca aggcgtaaca ggccacgggg
    36421 atcagcagtg agcccagatt agcgctggct aggatggcaa acatcgttcc gcccgttgcc
    36481 agagcgagca gcagggctcg ccaggccggt tgcgtgcagt cgctcaggta ggcgcgcaga
    36541 taggccagat gggcgagaag gaaggcgcct aggccgaaaa caaacagatc atcgggccag
    36601 tcgagcagga tatcgcccgc caatgagcaa agcaggccga ggctgagcca gcgtcgatac
    36661 aggccgctgg gtgcagtcct cagccacagt agcagggcaa ttactgggat gcccttgctg
    36721 agcagaccca gctcgctcag gccgcttagc cgtgcataga gaaaaactgc agcgcccagc
    36781 aaacccagca gcagataacg catggccttg tccttgatgc tcgaaaggcc agcactatgg
    36841 cgtgtcgcga attaactggc cacgccgtgg gtgccagtca aagcagcatt tcgcgccagt
    36901 tatcgcgcct tgattgtgca gttgaaacgc tgggcgggca ctacgccgac ttcccagggg
    36961 cgttcatagg tcagggccag ttggccttcg ccagccgtga tgacctcgaa gcgccaggtg
    37021 gaaaccccgg cgctgccgac cagaccggcg tcttccgggt tgctgtaaac ctcggggccg
    37081 aggcttttca gcacttgcgg ggcggagtcg cgcagcaacc agcggaagcc ggtggtgggg
    37141 ttgctgggca ggcgcagatt gaattgctgg ccgacgtgca gcgacagcgg gcatttgctc
    37201 tgctggcttt gctccagggt cacgctgctg ggcttttgcg cgcaggcggc gagcagtgca
    37261 aggctgagtg ccactgtcag gcggggggca aacatggtga gctcctggcg aaagtcgagg
    37321 ctgccagcat agccgctagg gctgctctgc gggagcccgg gctgcaaacg ccgcctgcaa
    37381 tggcgcacgc aagcgctggg cgtaatcgtc gatcagcggt gtgagcaggg caaaacattc
    37441 gtggggtgcc aggcgtacgt cgcggctgtc gatcagctca tgctccaggc gttgcagcac
    37501 ctgctgcaaa gatgcgccga cgggcgactg ttccagttcc aggcagagct gctgaatctg
    37561 ctgtttcagg cttggcgggc attgactgta attggccgca cgcacgctca ggccgcgcag
    37621 gcgggcgagg ttttccaggc catggcaggc ctgggcaatg ccctctacgt gtgcgttgcc
    37681 agtttgatgc aggcgggttt cgagtgttgc gataaccgcc agcaagccgt cgataacctg
    37741 gcaatgcgcg gcaaagtccg ccgggttgcg ccgcagacgt ggccagtcct gctgcaacgg
    37801 ggcgagtgcg aggcttgcgc cgggccagtt cagccagagt tgatcaagct ggcgggccag
    37861 ggcgttgcgc tggctggtgc tgggcaaatc ttgctgggcg cccaggccgc ggtgtttttg
    37921 caggttctgc agcaggtcga tggcgcgttg cagcaacgca gcttgcagct cgttgagatg
    37981 ctgctggtgg cgtttgccgg caagctggct gaggaagtgg ccggcgagta atagcgccaa
    38041 tgccagagcg agagcaatgc tgagggccat aagatgtttt cctgaacccg aacggtgcgc
    38101 aagggggcag gtacatgcag caaatccctt gccaggccgc gcaaaggccc gatttcaggc
    38161 gaccttaatc cactggcggc cgactatttg ccccatatac gggcgcgttt aatgtctctg
    38221 agaatttttg gtgcggccgc tgcccatttg tcgtgcgggc tgcagtttta ccgggcaaag
    38281 agttaaagtt gcacctctgt ctttctgggt tgcacttttc atgttcaacg cgcgtttgat
    38341 gcgtcttgat gaccaggccc atgagcacac ccacgaccat catcaactgg tgctgtcgct
    38401 ggccgggcgt gccgaatttg aggtgaacgg ccgagggggc gaggtgtgcc ggatgcgcgc
    38461 ctgcctggtg ccaggggatg ccgatcacca gttcgccggc atgggcgata accgcatgct
    38521 gatcatcgat ctggatgagc agggcacctc cagcgaagac ctgcaactgc tgacccgcct
    38581 gttcgaaacc ccgcgttacc cgcagttgga cgccgacttc cagaacctgc tgagctatgc
    38641 cggcgccgag cttgaacgtt acggtaccga tccgctgttg ccgcgcgcgc tgggtggcgt
    38701 gctgctgcgc gccttgcacc tgcgcctgtt tggcgaaccc catgcgcggc ccgtgggggc
    38761 gctgaatctg gagcggatcg acagttatat cgttaagcac ctggcgcgac gtatcagtgt
    38821 ggctgagctg gcgcaggtgg cttgcctgag ccccagtcat ttccatgcgc agttcaagga
    38881 cagcgtcggc ctgacgccgc atcagtacct attgagaacc cgcctggacc gcgccgtgcg
    38941 gctattgcgc gagagcggcc tgccgctgat ccgtatcgcc gaagagtgtg gtttttccag
    39001 ccagagcgcg ttgaccaccg ccatgcgccg ctacctgggc ctaaccccga aacgcctgcg
    39061 cagcgccgag taaacacgcg ctggtcgtct tggatggcgg ggcccagcag gcgggcgccg
    39121 ccgatcaaag gtgaacttct tcacgctggc gactaagctg tggcacacat agacatttac
    39181 tgtgcctggg caagaatgct gtccacttca agtgtgcccc taaagtacga gtcgtcttga
    39241 tgagcaaaca cctccaccta ttgttcgcat tttcactgct cggcatcttg ctgttcagtg
    39301 caccgctgca tgccaccaga ttgcaagctg agttggtgga tgaccaaggg caacccttgg
    39361 ccaatgccgt gctcagtttg cgtagcgcgg ttgcacctga ggttgcacct gctgctgcgg
    39421 tgatggatca gcgctcgcag cagttcgcgc caaatgtgct ggccgtgcgc agcggcacct
    39481 cggtgtcatt tcccaatagc gacaatatcc gtcatcacgt ttattcgttc tccctggcca
    39541 agcgctttga actgcgtctg taccagggca caccgagcga gccggtgatt ttcgacaagc
    39601 ccggcgtagt agtgctgggc tgcaatatcc acgactggat ggtgggttat gtctacgtga
    39661 ccgacgatcc ctggtttgcc gttagcgatg cgcagggcaa gctgaatctg gagcagctgc
    39721 cggcaggggc gtatacggtc agcctgtggc acccgcaagc gccggatatg ttgccgcagg
    39781 ctgccggcca ggtgcagctg accgaggccg gcagccagca gcgctttacc ctggccatgc
    39841 aggcgccagc catccaggca gcgccgagtg cgccggccag cgcctttggt gatgcgttca
    39901 agaaggctcg cgatgctgcg caatagtttt caggcgcgca tcgccggcgt actggtattg
    39961 ctgctgttgg tggtgatcgg cgcgctctat gcggccgtgc aaaccgccac cagtgctgcc
    40021 gtgcgcagcc aggcccgtga gcagttggat gtcggcagtc tggtgtttca gcagttgctc
    40081 gaagtgcgtg gccgccagtt gcatgatgcg gtgcaggtgt tggccgccga ttttggtttc
    40141 aaggatgccg ttgccagtgg cgacaccgag accattcgct cggccctggc caaccacggc
    40201 gcgcggatca acgccagcgc agtgatgatg ctggggctgg atggcaatct ggaggtcagc
    40261 agtgatcggc agatcagtgg cgaaaccgcc cagcgcctca gtgcccaggt gatgcagcgc
    40321 cagcgcgagg gcgtgcagat attcctgatg ccgattgccg acaagatcta cctgttggtg
    40381 caggccacgg tcaccgcgcc gctgccgatt gcccgggtga tgatgggctt tcaagttgat
    40441 gatgtattcg ccgccgaatt gcgcgagctg actcacctgg acctgaccct gctggcgacc
    40501 caggacggcg cggctgatat ctggatcagc accctgcagc cggcgcagcg tgagcaactg
    40561 caaggcagca tggccgaact ggctaatgat gcggtaagcc tgatcggtga cgagtcttac
    40621 ctcaatcaaa ccctggtact ggccagtggt gacggctttg aagtgcgcgc gctgctgcac
    40681 aagtctctga gtcaggcgca gcaggcgttt gcgccgctgg atcaaaatat tttgctgatt
    40741 gctctggtgg cgctgactgc ctcattgggt ggcgccttgc tgctggcgcg ttcagtgtcg
    40801 cagccagtgc agcaattggc ggccgtcgcc gagcgggtgc gccagggcga ttatcaggcc
    40861 agcctagatc tgcagcgcgg tgatgagttg gggcgtttgg ccaccgcctt caaggccatg
    40921 cagcaaggca ttgccgagcg tgagcggcaa ctggcgcaca acgcgctgca tgatgcgctg
    40981 accggtctgc ccaaccgcac tctggcgctg gagcgtctgg gcagtgcgat caccgccggt
    41041 cggccgacgg cgttgcttta cctgggcgtg gcgaattttc gtgcggttaa tgaaagcctg
    41101 ggcgcggagg gcggcgacct ggcgttgcag cagctgagcc gacgcctgca ggcgattctg
    41161 cgccctggcg atagcgtggc gcggattatt ggcgatgagt ttctcttgct gctggaaaat
    41221 accgacagcg atagcgccgt gggcattgcc gacaaagtgc agcagctgct gcttaagccg
    41281 ctgcgcgtcg ccacccatga tttcgccctg gattgccgca tcggcattgc cagctacccg
    41341 gtcgatggca acgccccgga agagttgctg cgccgggcaa gtatcgccat gcaggatgcc
    41401 gccggcatgc ccagccatct gcaggtctat cagcgcggcc gcgatgatgc ccagcagcgg
    41461 cagattcgcc tgattcgtga tctgcgccat gcgccgggca aggccgagct gctgttgcac
    41521 taccagccga agctggacat tgccgccggc caggtgcgcc aggccgaagc tttactgcgt
    41581 tggcagcatc cggaactggg catggtttcg ccgggggaat ttattccgct ggccgagcgc
    41641 accggcagca tccagctgct gactacctgg gtgatcgagg aagtcctgcg ccagttacgc
    41701 gaatgggctg ggcgcggcct gtgcgtgcag gtgtcgctga atatctccac cgaagacctg
    41761 atcgacccac agttaccagc gcgggtcagc aaactcttgg ccgagtacca ggtgccggcc
    41821 gagcagctga tttttgaaat taccgaaagc ggggtgatgc ttaatcccga gcttgctttg
    41881 caggtgctgc atgatctgcg ggcgtgcggc atcagcctgt cggtggatga ctttggcaca
    41941 ggctactcgt ccctggcgca gctcaagcgc atgccggtgc aggagttgaa gatcgatcag
    42001 tcgtttatcc gtgacctgga cgatgccagc gaggattcgg taatcgtgcg ctcgaccatc
    42061 gagatgagcc acagcctggg cctcaaagtg gttgccgagg gcgttgagtt cgagcgcagc
    42121 ctgcaactgc tggggcgctg gcagtgcgat accgctcagg gttacctgat cagtcggccg
    42181 ttggcggcgc aagcctttga aacctggatg gcgcaataca cccatgcctc ctcgaccctg
    42241 gtgcattgac catgactcaa ctgccacgct gcattgccct gttgcttggc ctgctggcca
    42301 acctggcctg ggccgatggc cgcttgctgg ccacgggtgg cgccagcagc attgaaggtg
    42361 cggcaggtgg cgggattaca ccctgggcgg tgctgtccgg ctatggcgag cgcggcgagt
    42421 ggggcgcgga tgtattcgct acgcgggtgg aaaccggaga ttaccgcctg gatgtggccg
    42481 gtgttggcgt agccttcgat aaccgtatcg agctgtccta cgcccgtcag cgttttgatc
    42541 tgggcaccct ggcgcgcgac ctgaatctgc cggaaaacag cctgagccag gatatcttcg
    42601 gcctcaagct gcgcctgttt ggcgacctga tttacgacca gctgccgcag gtgtcgctgg
    42661 gtattcaaca caaacgccag aaggattttc tgattcccag cctggtgggc gcccagcgca
    42721 gtgaagacac cgagggttat atcagcgcca gccggctgat cctcggcggc gcctttggtt
    42781 acaacctgct gctcaatggc ggtctgcgtt acagtcgggc caacgagctg ggtctgctgg
    42841 gttttggtgg ggatcgccgt gatcgccatt ccgtgctcaa ggaaggctca gtcgccgtgc
    42901 tgttcaaccc gcgctgggcg ttgggtgtgg agtatcgcga gaagccggac aacttaagct
    42961 ttgccggcga aagtgactgg gccgatctgt ttctcggtta cttccccaac aagaatctgg
    43021 ccgtggtgct ggcctatgcg cgtctggggg aaatcgccac gctggacaat caggacgggg
    43081 tttacctgtc cgtgcagggg agtttctaag tatgcgcagt ctgttgctgg tggttgttct
    43141 gagcctggcg gcctgtgccc agcagccggc caaagacgac agcctgtacc gcgacctggg
    43201 cgagcaggct gggattaccc gcatcgtcga aggcatgctg ctgaatattg ccgccgaccc
    43261 gcgcatcgtt cggcatttcg agaacatcga catcgtgcgc ttgcgcgaca agctggtgga
    43321 gcagatctgc gttgaagccg gcggcccgtg cacctacacc ggcgacagca tggaagaaag
    43381 ccacaagggc cagaacctca cacccagtga cttcaacgcc ctggtggaaa acctgcagga
    43441 tgcaatgagc gcccagggcg tgcccatgcc agcgcaaaac cgcctgctgg cgcggcttgc
    43501 gcccatgcgt gcgcaggtga ttgatcgctg agcgctgtct gcgactgctg ctgtagcgtt
    43561 gcgcctggcc gctattcagt accttgagtt ctgttctggc tgcagctgag ccaacgctgt
    43621 atcgaccagc gccaacagct cgtcgatggc ctgggtagcg cgttggaagt agctttttgc
    43681 ggcgatgtgc ggttgttcct ggccgagcaa ttcctgctca atgcagtaca agaaactcgt
    43741 cactacctgt tcccgctgag ccaggcggaa ggcgtcgcgc tgcggatgtt cggctgtatg
    43801 ccggtgcagg gtggcgaagg ccgtatcggc caggagttga attttctggt agaggaaacg
    43861 caaccgcacg cgctgctcga cattgctttg tccggcggcg gcaatgccgg tgcccagagc
    43921 gcgggcctgg cccgcccatt ccgcagcctg taccacttcg cgccagatgc acggtagata
    43981 tgctagcgcc gctcgaccct cgctaaggtc gacttcgctg gcgacatcct ccatcagaaa
    44041 gatgctgttg cggatgatca ggtggtgctg ggccaagttg ttcgctgggt cagcactatg
    44101 cccctcacgc aggcgcgacc aatgatcaat caggctgttc caagcttcgc gctggttgct
    44161 gctgagtgtc tgggcgtcgc ggatatgtcg gtccagctgc tggcgcatgt tggccaggtc
    44221 ttggctcatg ctggtgtcgc cacagagcac ggcatggctc aggccgcgat gacgttgcaa
    44281 gtcggcgagc aatgcgcgca gcagcactag ttggcggatg ttcgtttgaa tgcgtgcttg
    44341 tttctggcgc aacaagcgcc ggtgcatacc gtagagcgcc aggccgatta cggtcagggt
    44401 ggcgacgcag gcgagcagag tgaatccgtg taagccatcc atgctgatta ccctgtcaga
    44461 tgagccagat gatgggcgat gctgcgagcc tgatcggccg cgcgcaggtt gtcggcatta
    44521 ccttggcgta cgtggtcggc cagttcggcg atctcagcta cggccttgcc ctgttgttcg
    44581 gtgctgaccg ctacctgatc tacctgctcg gtcagtcgcc gggtgcactg ttggacctgg
    44641 ttgagcaagt ggcgcacagc ctcagagctc tgggcgccgc gctgggccag ctcgctaagg
    44701 ttggcgacct gcaacgtggc gccctggatg tgctgctgca ccgagtggat gttgcggccg
    44761 atctctgccg ctgactcctg gctgtgtatc gccaggcggc gtacttcatc ggcgaccacg
    44821 gcgaaacctc gaccgctctc gccagcacgc gcggcttcga tggcggcgtt cagtgccagc
    44881 aggttggtct gatcggcaat gctgcggata gtgccggaca tctcattgat ggtgtgcgag
    44941 ttactgttca gttgctggat cagctggttg gtggcctgcg cctggcgggt catctccgcg
    45001 acttgctgca ccagctcggt gaggcggtcg ataccgtcgg tcaactgctc actggctacc
    45061 aggctggtct gccgcgattc atcggccagt gccgccacct gtacgatgct gacattcaat
    45121 tgctcgactg cggcggcggc catgctggct gagtcgctct ggtttccggc attgcgagtc
    45181 accagctcgg cgcttttttc gagttcctga gaggagtggg cgatctcgtc caggcgttgt
    45241 tgcagcagct gttgctggcg ctgttcacgc tgcaagcact gctgcaggct acggccaatc
    45301 ggttgtagca gttgccactc gttagtacgc ggcgcctgcc ggccggtctg ttgcaccgat
    45361 gcgtcgcagt aacgttgcag ctgcagtact tcgcgccaca gcaagtacag gctggcaatg
    45421 ctcaggtagg ccagcaaggc ccatatcgac gcagcgaaat gggttggaca tagcaccgtg
    45481 gtggcaatgc cgaccagggt gatgccgagc aggctgcact gcatgccgac aacgcccagt
    45541 gcgcgcagca acaacagcgc gggataggta agccagttca tccgtgggtt tctccggtag
    45601 tgccgtagct gttggcgcca aaaaaaacgc ctgaaatcct gcaaagcaaa ttgcagtgag
    45661 ttcaggcgcc gttgcctggc agagcggctc tgccgaaatg atcgttgtga gtggcattta
    45721 tgcatgagca atgccatgta ctgatggcac gttgctattg ttactaaaac agcgacaggc
    45781 cgcgctttgg ggcggatgtt cgagagttgc attgataacg cgcggcggtg gattgacgcc
    45841 agaatagtgc gctggcacgt tctactgcgc gaatatcgct cagcgcaagg tctgcgcgat
    45901 agccagaata tcggcgcggt ggctgatcac caggctgagt tcgccgttct caccgataaa
    45961 gtcctgcggt ggcatcagtt tctggtaagc cgccagctcc tgcagtggcg gcaggtcggc
    46021 atctggggtg cgttccttga tggcgtggta gagcgccagg ttgaccaggt cgagcaattg
    46081 caggctatgc ccggggctgc gttgccagtt gccggtgtcg cggatgatct cgatatattc
    46141 gttatgcacc gaccatttct tcagcacgat aatgccgagt gatttgctgt attcgcggca
    46201 cagcttgtag tacagctcgc tggagggggt tacgctttca tttctgaatg ccgagagcac
    46261 ggcgagggtg cctacttcac tgagcaggct ggccagcagg gcatgctcgg ctgtcacatg
    46321 gcccagcagg cgggctagca tcgcactggt gctagccttg attaccaagc gctcccaggc
    46381 atcgacaaac agccgcttat gcccagggct gtgcagggta aacaggcttt tgatgctgtg
    46441 caccatggtg atccggtcga cttccgtcat gccgagcagg ccgatcactt cgcgcagcgt
    46501 cttgggtgcc aggctgtggc gatacagtgc gctggacgca tgtttcatca gcatggcgct
    46561 gagagctggg tcgcggctga tcaaacgcac cagggcattc agggaaatat tcgagtcacc
    46621 tagggcgcgg cggatatcca gggtaatggt cggcaggctg ggcaattgct cttcgccctg
    46681 cattaactgc gagacgacgc gtcggtagat tgagtaatcg gatgcttcgc tctgcaacac
    46741 acacctccag ccttatggat gcagtctaga tggccgttac acactcggta taagtgacga
    46801 tggatggcct tgattggcga aatctttggc cttgcgcttt aaacagctgt ttgcagtaag
    46861 ttcctgtgcc tggagttttt tgcaaaaact ccagagtttt ttgtaagaag cggtcatgcc
    46921 tctgccacta aattccgtgc tcggtttacg cggtgcgcag gtttctgccc acaagatgct
    46981 tcggtaaacc tctgccgctg ggttagatct aatacaggca gtgagcgaat aaaggacagg
    47041 tgcgaccgta atcgcccgtc tggccttttt gaaagtgcgc ctgtgcagtc tgcgctgagc
    47101 aagaattttt acgggcgact gtcatgcggc atgcgcccgt tattgtattg gggcttttgt
    47161 gcctcggttg cggtaagcgg cttcacctgc ataggcaggc gtgcaatgcc acgttggcaa
    47221 tggggctatc cctgttgctt gaataacaac aatcaagagg ggagctcccc aatgactgca
    47281 aatactccga tgctcgtcac cttcgtggtg tacatcgcgc tcatggtgct gattggcctg
    47341 ttcgcgtaca tgcgtaccaa gaacctttcc gattacatcc tgggcggtcg cagcctgggt
    47401 agtttcgtca ccgccttgtc ggctggcgca tcggacatga gcggctggct gctgatgggc
    47461 ttgccgggcg cggtatacct gtctggtcta tccgaaggct ggatcgccat cggcctgatt
    47521 gtcggcgctt acctgaactg gttgttcgtt gctggccgtc tgcgtgtgca gaccgagcac
    47581 aacggcaacg ctctgaccct gccggattac ttcacccacc gctttgaaga cagcagccgc
    47641 gtgctgcgca tcttctccgc cctggtgatt ctggtgtttt tcaccatcta ctgcgcctcg
    47701 ggtgttgtag cgggtgcccg cctgttcgaa agcaccttcg gcatgtccta cgagactgcg
    47761 ctgtgggccg gtgctgcggc gaccatcgcc tacaccttta tcggtggttt cctggcagtg
    47821 agctggactg acaccgtgca ggccacgctg atgatcttcg ccctgatcct caccccaatc
    47881 gtggtgctga ttgccaccgg cggtgccgat gcgacctttg cggcgattga actgaaagat
    47941 gccaccagct ttgacatgct caaaggcgcg acctttgttg gcgttatttc gctgatggct
    48001 tggggcctgg gctatttcgg ccagccgcat atcctcgcgc gctttatggc cgctgattcg
    48061 gtgaagtcga tcccggcagc ccgtcgcatc tccatgactt ggatgatcct gacccttgca
    48121 ggcgccgtgg ctgtgggctt cttcggtatt gcgtacttct ctgccaaccc ggacctcgct
    48181 ggcccggtga ccgagaaccc tgagcgcgtg tttatcgaac tggccaagct gctgttcaac
    48241 ccgtggatcg ccggcatcct gttgtccgcc atcctggctg cggtcatgag caccctgagc
    48301 tgccagttgc tggtgtgctc cagtgctttg accgaagact tctacaaagc cttcctgcgt
    48361 aaagatgcgt cgcaggtcga gctggtatgg gttggccgtg gcatggtgct gctgattgcc
    48421 atcgttgcca tcatgctggc tgctaacccg gaaaaccgcg tactgggcct ggtgtcctac
    48481 gcctgggcag gtttcggtgc tgcgttcggt ccggtggtga tcctctcgct ggtctggaag
    48541 caaatgactc gcaacggcgc gttggccggc atgctggtcg gcgccatgac tgtaatcctg
    48601 tggaagaatt tcctcggcca cctgggcctg tacgaaatca tcccgggctt tatcttcgcc
    48661 accatcgcca tcctggtgtt cagcaagatc ggtagcggcc cttcggccag catgatcaag
    48721 cgcttcgacg aagccgagaa ggaataccag tcggtctgat ggtttgaaat acccggcaac
    48781 tgactggtcg ctcagttgcc ccgcggaccg atttcgcggc gatgtgcgtg cgacccgata
    48841 gtgcttcgcc ggcccacacc tttggtgttg ggccggcttt tttatgggcc cgcaaaagtc
    48901 gctgtgtcgc ctttgggctt tgcgtcaggt gcgcgcttgg ctaaggtggc aggcattgtc
    48961 catcgagctg ccgcaaaaac aggttctgca tgactcaatc accgaaaaac ccactgcatg
    49021 gcgtgaccct tgaagccatt ctcaccgccc tggttgccca gtacggctgg gacggcctgg
    49081 ccgagcggat tgatatccgc tgtttcaaga gcgacccgag catcaagtcc agcctgacct
    49141 ttttgcgcaa gacgccctgg gcaagggaga aggtcgaagc gctgtatatc cgctcgcaag
    49201 cgcgtaaata ggcgctaagc gccggttcga taatctggca cccttgttgc aatacctctg
    49261 caaacgccgc gaagcgtcaa aaaaccgcgg ccagtggagg tagagatgag ccagggtgtt
    49321 gaattcaatc gcttgatgct ggagatgcgc tccatgcagg ccgacgccat ggcgcgtcag
    49381 aagcctgtca ccagcacgcc ggagcccggt gcgccgagct tttccgagat gctcgggcag
    49441 gccgtgggca aggtcaacga aacccagcag gcctccaata agctggcaac cgcctttgaa
    49501 atgggcacta gcggcgtgga tttgacggac gtgatgatcg cctcgcaaaa agccagcgtt
    49561 tccatgcagg ccatgaccca ggtgcgcaac aagctggttc aggcttatca agacattatg
    49621 cagatgccgg tttaagggta gggctgaatc atggctgaag ccgtcgttgc aaacgtacct
    49681 gccaccactg gcggtgatga tccgaagaag ccgctgtttg gcctgacctt tctggaaaac
    49741 ctcgccgata tgtcgatgtt gcgccagatc ggcctgctgg tcggtctggc cgccagcgtg
    49801 gccatcggtt ttgccgtggt gttgtggtcg cagcagcccg attatcagcc gctgtatggc
    49861 agcctcgacg gtatggatgc gtcccaggtg atggagacgc tgtccgccgc tgatatcaaa
    49921 tacaccgtgg aacccaattc cggcgcgctg ctggtcaagt ctggtgactt ggctcgcgcc
    49981 cgcatgcgcc tggcggctgc tggcgttgcg ccaaaggaca atagcgtcgg ttttgaaatt
    50041 ctcgaccagg agcaaggcct tggcaccagt caatttatgg aagccacccg ttaccgtcgc
    50101 ggtctggaag gcgagctggc gcgcaccgtt tccagcctga acaacgtcaa ggcggcccgc
    50161 gtacatctgg ctattccgaa gagctcggta ttcgttcgcg atgagcgcaa gcccagcgcc
    50221 tcggtgctgg ttgaactgta ttccgggcgc ggcctggagc cgagtcaggt gatggcgatc
    50281 gtcaatctgg tcgccagtag cgtgccggaa atggacaagg cccaggtcac cgtagtcgac
    50341 cagaagggca acctgctctc ggatattcag gaaatgtccg agctgaccat ggccggcaaa
    50401 cagttcgatt acagccgccg tatggaaagc ctgtttaccc agcgcgtgca caacatcctg
    50461 caaccggtac tgggctcggg tcgctataag gccgaagtgt cggcagatgt ggatttcagt
    50521 gcggtggagt ccacctcgga gatgttcaac cccgatcaac cggcgctgcg cagtgagcag
    50581 tcggtcaacg aacaacgcac cagcagcctg ccgccacaag gtgtgccggg tgccctggcc
    50641 aatcagccgc caggtgcggc ggcagcgcct gagcaagccg gcggcgcggc tgcggtgcct
    50701 ggcccggttg ccgcggggca gccgctgctg gacgtcaatg gtgagcagat catcgacccg
    50761 gccaccggtc tggccatgct ggcgccgtat ccggccgaca agcgcgagca atccacccgc
    50821 aacttcgaac tggatcgctc gatcagctac accaaacaac agcagggccg tctgcgtcgg
    50881 ttgtcggtag cggtggtggt ggacgatcag gtcaagatcg atgccgccag cggcgaaacc
    50941 acccgggtgc cgtggagcag tgatgacttg gcgcgcttta cccgcctggt gcaggactcg
    51001 gtaggcttcg acgccagccg gggtgacagt gtcagcgtga tcaatacgcc attctccgcc
    51061 cagctgggcg aggaaatgat cgaaattccg ttctacaccc aaccgtggtt ctgggacgtg
    51121 gtcaaacaag tgctcggcgt gctgtttatc ctggtgctgg tattcggtgt gttgcgtccg
    51181 gtgctcaata acatcactgg cggcggtcgc ggccagggcg atggtcgcgg tggcgatgtc
    51241 gagctgggtg aaatgggcgc gctgggcggt gatctggccg aggatcgcgt gagcctcggc
    51301 ggcccgcaaa gcatcctcct gccgagcccg agtgaggggt atgatgcaca attgaacgcc
    51361 atcaagggcc tggtcgctga agacccaggc cgcgtagctc aggttgtgaa ggattggatt
    51421 aacgccgatg agtgacaatc gaaccgccac taaattgagc aaggtcgaca aggccgcgat
    51481 tttgctgttg tcgttgggtg aaaccgacgc cgctcaagta ctgcgtcacc tcgggccgaa
    51541 agaagtacag cgggtcggcg ttgctatggc cggtatgcgc aatattcagc gcgagcaggt
    51601 tgagcaagtg atgggcgaat ttgtcgacat cgtcggcgat cagaccagcc tgggcgtcgg
    51661 tgctgacggt tatatccgca agatgctcac tcaggcgctg ggcgaagaca aagccggcaa
    51721 cttgatcgat cgcatcctgt tgggtggtag caccagcggt ctggacagcc tgaagtggat
    51781 ggagccgcgt gccgtagccg acgtgattcg ctacgagcac ccgcagattc aagccatcgt
    51841 ggtggcttat ctggacccag accaggccgg cgaagtgctt ggccacttcg accacaaggt
    51901 gcgtctggat atcgttctgc gcgtatcgtc gctgaacacc gtacaaccgg cggcgctgaa
    51961 ggagctcaac cagattctcg agaagcagtt ctccggcagc tccaacacca cccgcgccgc
    52021 gctcggcggg gtcaagcgtg ccgccgatat catgaacttc ctcgacagct cggttgaagg
    52081 ccagttgatg gactcgatcc gcgaagtcga cgaagacctc tccagccaga tcgaagacct
    52141 gatgttcgtc ttcgacaacc tcgccgatgt cgacgaccgt ggtatccagg cgctgctgcg
    52201 tgaggtgtct tcggacgtgc tggtgctggc gctcaagggc gccgacgagg cgatcaagga
    52261 aaaagtcttc aagaacatgt ccaagcgtgc cgccgaactg ctgcgcgacg atctggaagc
    52321 caaaggcccg gtgcgcgtca gcgacgtgga aaccgcgcag aaggaaatcc tcaccatcgc
    52381 caggcgtatg gccgaagccg gggaaattgt cctgggcggc aaaggcggcg aggaaatggt
    52441 ctaacgcatg tccagcaaag agccacccag cgagctgatt cgcgccaagg acctcaaggg
    52501 cgttgatgtg tgggcgctgc ccagcttcga cccggaagtc gaaatacctg agccagaaga
    52561 acagcaggcg tccgagcagc ctgttgatac gggtgagcag gtgccggccg aaagcgaaga
    52621 ggtggccctg gaagacgtca aacccttgac gttggatgag ttggaggcga tccgtcagga
    52681 tgcctacaac gaaggttttg ccactggcga gaaagacggt ttccatgccg gccagctgaa
    52741 agccaagcag gaagccgata tcgccctggc cgcaaaaatg gccgggctgg agcagttgat
    52801 gacccagttg ttcgagccga ttgccgggca agaccagcaa cttgaagaag ccatggtcgg
    52861 cctgcttggg caaatggtgc gccaggtcat tcagcgtgaa ctggtcagcg actccagcca
    52921 gattcgccaa gtgctcagcg aggcgctcaa gctgctgccg atgggcaccg gcaatatccg
    52981 cattcacctt aatccgcaag acttcgagat gatcaaggcc ctgcgtgagc gtcacgaaga
    53041 aagttggcgg attctcgaag acgcttcctt gctgcctggc ggttgccggg tggaaagcga
    53101 actcagccgt atcgacgcca ctgtggaaac ccgcctgaac caagcgatca agcagttgtt
    53161 cgagcaacag cgcgaacagg cgtcgcatcc gctggaagcc gatttgcata ccgacctgga
    53221 tgccctgccg gtggatgccc ctcaggtgaa tagccaggcg agcccggatg cgccttgacc
    53281 gcgtgagttt cgccaaacgc ctggccggtt acagcgaggc cgtggcgctg ccaactcagc
    53341 cgttggtcga aggtcgcctg ctacgcatgg tcggcctgac cctggaagcc gaaggcctgc
    53401 gcgccgctct gggcagccgc tgcctggtaa tcaacgacga cagctatcac ccggtgcagg
    53461 tcgaagccga ggtgatgggt ttttccaacg gcaaaatctt cctgatgccc gtcggcagcc
    53521 tggccggcat cgccccgggt gcccgtgtgg tgccgttgcc ggataccggc cgtttgccca
    53581 tgggcatgtc gatgcttggt cgggtgctcg atggcaccgg ccgcgccctc gatggcaaag
    53641 gcgggatgaa agccgaagac tgggtgccga tggatggccc ggcgatcaac ccactcaatc
    53701 gccacccgat cagcgagccg ctggatgttg gcatccgctc aatcaacggc ctgctaacgg
    53761 tgggccgtgg tcagcgccta ggtctgtttg ccggcaccgg cgtgggtaag tcggtgctgc
    53821 tcggcatgat gacgcgcttt accgaggccg acattatcgt ggttgggctg atcggcgagc
    53881 ggggccgcga agttaaggaa tttatcgacg agatcctcgg ccacgagggc cttaaacgct
    53941 cggtggtggt ggcttcgccg gccgatgatg cgccgctgat gcgcctgcgc gcagcgatgt
    54001 attgcacacg tattgccgaa tactttcgcg acaagggcaa gaacgtcttg ctactgatgg
    54061 attcgctgac tcgtttcgcc caggcccagc gcgagattgc cctggccatt ggcgagccgc
    54121 cggcgaccaa gggctatcca ccctcggtat ttgcccgcct gcccaagctg gtggagcgtg
    54181 ccgggaatgc ggagaagggc ggtggctcga ttaccgcgtt ctacaccgtg ctcagcgaag
    54241 gtgacgacca gcaggacccg attgccgacg ccgctcgtgg tgtgctcgat gggcatatcg
    54301 tgctgtcgcg ccgactggcc gaagaaggcc attacccggc catcgatatc gaggcctcga
    54361 tcagccgggt gatgccgcag gtggtcagcg ccgaacacat gcgcttggcg caacgcttca
    54421 agcagttgtg gtcgcgttat cagcagagtc gtgacctgat cagcgtcggc gcctatgtac
    54481 caggcggcga tgccgacacc gacctggcca ttgcgcgcca gccggccatg gtcaaatacc
    54541 tacggcaaaa cctgcaggac aactaaagcc tggaaagcag ca
//
LOCUS       DASRUM010000288         2664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2315013, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000288 DASRUM010000000
VERSION     DASRUM010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2315013"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VFF22_19305"
     CDS             complement(<1..589)
                     /locus_tag="VFF22_19305"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HZX19002.1"
                     /translation="MADEWKASAAADVDGKSWQLLEKTLLAGVQEQRRARRWGIFFKL
                     LTFIYIFGALALFSPMLAMKKGATTGSHTAVIEVRGMIADQESASADNIVSSLRAAFE
                     DTNTKGVILRINSPGGSPVQSGYIYDEIRRLRAEYPAIKLYAVITDLGASGAYYIASA
                     ADEIYADKASLVGSIGVTAATFGFVGVMEKLGVDRR"
     gene            complement(579..1289)
                     /locus_tag="VFF22_19310"
     CDS             complement(579..1289)
                     /locus_tag="VFF22_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016493668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZX19003.1"
                     /translation="MRDCLVVDYQLLIFDWDGTLVDSIGRIVAAMHMAADACELPRCT
                     DEQVRGIIGLGLPEAIRCLYPDMHDALRAQRLRQSYSENYLALESEPSALFHGVAEGL
                     EAFRAEGYRLAVATGKGRHGLQRVLADRGWLDYFDYSRCADETASKPDPLMLHEILAQ
                     SGVAADRALMVGDSSFDLLMAQRAGMDCVAVGYGAQPLQALRECQPTLAIECFTELRA
                     WLARRASNQSVEVVLHGR"
     gene            complement(1282..2235)
                     /gene="rluC"
                     /locus_tag="VFF22_19315"
     CDS             complement(1282..2235)
                     /gene="rluC"
                     /locus_tag="VFF22_19315"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HZX19004.1"
                     /translation="MTTPTSPTSGVQLLEVAPELAGQRIDNFLRTQLKGVPKTLIYRI
                     LRKGEVRVNKGRIKPEYKLQAGDIIRVPPLRLAERDEPEPLAQGLLQRLEAAIVYEDK
                     ALIVLNKPAGIAVHGGSGLNYGVIEAFRQLRPDARDLELVHRLDRDTSGLLMIAKKRS
                     MLRHLHEALRGDGVDKRYMALVRGNWATAKKQVNAPLLKSNLRSGERMVEVNPEGKEA
                     LTIFRVLRRFGEFATLVEAKPVTGRTHQIRVHAQHAGHCIAGDSKYGDDDFTKEIRGL
                     GGKRLFLHAYELVVPLPEGGQLKLAAPVDEMWARTMERLGA"
BASE COUNT          629 a          860 c          666 g          509 t
ORIGIN      
        1 cgcggcgatc cacacccagc ttctccatca cccccacaaa accaaaggtt gccgccgtga
       61 caccaatgga gccaaccagg ctggctttgt cggcatagat ctcatcagcc gcactggcga
      121 tgtaataggc acccgacgca cccaaatcgg tgatcactgc atacagcttg atcgccgggt
      181 attcggcgcg caggcggcga atctcgtcat agatgtagcc cgactgcacc ggactgccac
      241 ccgggctatt gatgcgcagg atcacaccct tggtgttggt atcctcaaac gccgcacgca
      301 gactgctgac aatattgtcg gcactggccg actcctgatc ggcaatcatc ccgcgcactt
      361 cgatcaccgc agtatggctg ccggtggtcg cgcctttttt catcgccaac atgggcgaga
      421 acaacgccag tgcgccgaag atataaatga aggtcagcaa cttgaagaaa atcccccagc
      481 gccgcgcgcg acgctgctcc tgcacaccgg ccagcagggt cttctccagc agctgccagc
      541 ttttaccgtc gacatcagcc gctgccgacg ccttccattc atctgccatg caaaaccacc
      601 tcaactgact gattagacgc acgccgagct agccaggcgc gcaactcggt aaaacattct
      661 attgccagcg ttggctgaca ctcacgcaac gcctgcaaag gctgcgcacc atagccgacc
      721 gccacacaat ccatgccagc gcgctgcgcc atcaacagat cgaaggatga gtcacccacc
      781 atcagcgccc gatcagcagc aacaccgctt tgcgcaagaa tctcgtgcaa catcagcgga
      841 tccggcttgc tcgccgtttc gtcggcgcag cggctgtagt cgaaataatc cagccaaccc
      901 cgatccgcca acacgcgctg caaaccatgc cgccccttgc cagtcgctac cgccagacga
      961 tagccctcgg cacgaaatgc ctccaagccc tcagcaacgc catggaacag cgccgaaggc
     1021 tcggactcaa gagccaggta gttttcgctg tagctttgcc gcaagcgctg cgcacgaagt
     1081 gcgtcatgca tatccggata aagacaacga atcgcctcag gcaaacccag accaataatg
     1141 ccgcgcacct gctcatccgt gcagcgcggc aactcacagg catcagcagc catatgcatc
     1201 gctgcgacaa tccggccaat ggaatcgacc agagtaccgt cccagtcgaa gatcaacagc
     1261 tgatagtcaa ctactaagca gtcacgcacc gagacgctcc atggtccgcg cccacatttc
     1321 atcgaccggc gcggcaagct tgagctgacc accttcaggc agcggcacaa ctagctcata
     1381 ggcgtgcagg aacaggcgct tgccgcccaa accgcgaatt tccttggtga aatcgtcatc
     1441 gccgtacttg ctgtcaccgg caatgcagtg cccggcatgc tgcgcatgca ctcgaatctg
     1501 gtgggtacgc ccggtaaccg gcttggcctc gaccagcgtg gcgaactcac cgaaacggcg
     1561 cagcacacgg aagatagtca aagcctcctt accttcagga tttacctcaa ccatgcgctc
     1621 accggaacgc agattgctct taagcagcgg cgcattgact tgcttcttgg ccgtcgccca
     1681 gttgccacgc accagcgcca tataacgctt gtccacacca tcaccacgca aggcttcgtg
     1741 caggtgacgc aacatgctgc gcttcttggc aatcatcaac aggcccgaag tatcacgatc
     1801 caggcgatgc accagctcca gatccctggc atccggacgc agctgacgaa aggcttcgat
     1861 aaccccgtaa ttcaggccac taccgccatg cacggcgatg ccagccggct tgttcagcac
     1921 gatcagcgcc ttatcttcat agacaattgc agcttcaagc cgctgcaaga gcccctgagc
     1981 cagcggctca ggctcatcac gctcagccag gcgcagtggc ggcacacgaa tgatgtcgcc
     2041 ggcctgcagc ttgtattcgg gcttgattcg acctttatta acgcgcactt cgcctttacg
     2101 caaaatccgg taaatcaagg tcttgggcac acccttgagc tgggtgcgaa gaaagttatc
     2161 gatgcgctgg ccggcaagtt ccggcgcaac ctcaagtagc tgaacgccag aggttgggga
     2221 ggtaggagtc gtcatcgcgc aatcataaca attttttatg gaattgaagc acttaatcat
     2281 tgctgctata gtcgggaacg ccgccaaaag cggcttggtt tgcggatgat cgccaaaact
     2341 gccatcccca accaacgcaa cccatttggg acgtgaggcc gtccgacggg ttattcaacg
     2401 aaagaaaagc ctccaaggct gtgaataact cggaaataaa gcctttaagc catgatgcgc
     2461 aacctgcccc tctggacagt tgcggtaata gttaacccgc tgcggatttt cgcgagcggc
     2521 actcgagttt cagcgatacg tgtagggtgg agatgtacaa ctgttggtcc gcgtagcagc
     2581 tcgctttacc aagacgcttt atctcgtccg ctaccaacag ctgattcctc ctcctgactt
     2641 agtgctttct gttatcacag cgag
//
LOCUS       DASRUM010000289         6046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1663953, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000289 DASRUM010000000
VERSION     DASRUM010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663953"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1446)
                     /locus_tag="VFF22_19320"
     CDS             complement(49..1446)
                     /locus_tag="VFF22_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NorM family multidrug efflux MATE transporter"
                     /protein_id="HZX19005.1"
                     /translation="MLHPLRDELRAILKLAGPLIAAQLAHVLMVFTDTVMMGLLGPAT
                     LAAGGLGAASYSFVSIFCVGVIASVGNLVAIRHGANDAAGVTRLTQNGLWLGWGLALL
                     AGLALWNLRPALLYFGQKPENVSGGMQFLSTLVFALPGYMSFMALRGFTSAIGRPGPV
                     MAISIGGALANFVLNYALIKGWFGLPSLGLAGIGLVTAVVMNVMALLLAWHVLHHSAY
                     GPYPLWRGLLRPVWAELKELLRLGLPIGGTYAVESGLFAFAALCMGALGSTQLAAHQI
                     AIMSVYVAFMVPVGISYAVTFRIGQHFGAGRLLQARQAGRLGLGLGALCMLGFACLFW
                     LAPHWVVGLFLDHTDSAYAEVVTLAVALLAIAAWFEFFDGIQTIAMGAIRGLKDAKTT
                     FWVGLGCYWAVGAPLAWALAFPLGWGAEGVWWGLAGGLACAAIGLTLGFEWKTARLLQ
                     PTAAAFTPQPKRLVS"
     gene            1585..2514
                     /locus_tag="VFF22_19325"
     CDS             1585..2514
                     /locus_tag="VFF22_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZX19006.1"
                     /translation="MSQRLPPLYALRAFEAAARHSSFTRAGEELAITQSAVSRHVRTL
                     EEHFACRLFERRGRSLQLTETARMLLPGLRDGFEALERACNTLRVDDTVLRLKAPSTL
                     TMRWLLARLSRYRLLSASEVQLTSAWMDVDKVDFLHEPFDCAVLLSQGDFPEEWESTE
                     LFAEWLIPVCAPEALSEQPWDLPRLQTAELLHPTPDRRDWRQWLQAMGLADQVPLKGG
                     QVFDTLELGMVAAARGYGVSIGDLVMVAEDVAQGRLSLPWPSAVPSGCSYYLVWPKAR
                     RGQERFRRLRDYLVADVAAMQLPVVERVGNRAR"
     gene            2855..3646
                     /locus_tag="VFF22_19330"
     CDS             2855..3646
                     /locus_tag="VFF22_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HZX19007.1"
                     /translation="MSNNNTKQRPGARKPVVLMSMGAQERKGHAYQVMTHKYITPLVE
                     LSGCVPLLAPSCCGADDIEQYLSMVDGVYLTGAGSNIDPALYGQENLTPSKGQDPDRD
                     LFDIPLIRGALARGLPLFGICRGMQEINVALGGNIHQKVYEVPGMNDHREDTSAPVDV
                     QYADSHSVRALPGSWFAELMGHADFPVNSLHGQAVDQLGEGIEALAHAEDGLIEAIHL
                     PGAEQFTLAVQWHPEWQAAQNPHSVKIFKAFGDACREQAARRDSQ"
     gene            complement(3703..5199)
                     /locus_tag="VFF22_19335"
     CDS             complement(3703..5199)
                     /locus_tag="VFF22_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HZX19008.1"
                     /translation="MSLAIVHSRAQVGVEAPAVTVEAHLANGLPSLALVGLPETAVKE
                     SKDRVRSAILNCAFDFPPRRITLNLAPADLPKDGGRFDLAIALGILAASGQVPASALE
                     HLECLGELALSGTIRPVQGVLPAALAARAAGRTLVVPKANAEEASLASGLTVIAVEHL
                     LEVAAHLNGQAPIAPYQAQGLLRQVLPYPDLAEVQGQIAAKRALLVAAAGSHNLLFTG
                     PPGTGKTLLASRLPGLLPPLDEAEALEVAAIHSVASHTPLSAWPQRPFRTPHHSASGP
                     ALVGGGSRPQPGEITLAHHGVLFLDELPEFDRKVLEVLREPLESGQIVIARARDKVRF
                     PARFQLVAAMNPCPCGHLGDPSGRCRCSSEQIQRYRGKLSGPLLDRIDLHLSVPRETT
                     RLDANAEPGPSSAELAAQVVQARQQQLTRQGCANAHLDLNGVQQHCSLQSEDRQWLEN
                     ACERLGLSLRAAHRVLKVARTLADLDSAAHISRSHLGEALQYRPSSSA"
     gene            complement(5239..5508)
                     /locus_tag="VFF22_19340"
     CDS             complement(5239..5508)
                     /locus_tag="VFF22_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HZX19009.1"
                     /translation="MLPPKALLDALTTHASRLFNGDAPLPRGEFEAQLKVLLQSAFSK
                     LDLVSRDEFDSQMVVLARTRARLEALEAKVAEMEARSTDTPTTSE"
     gene            5935..>6046
                     /locus_tag="VFF22_19345"
     CDS             5935..>6046
                     /locus_tag="VFF22_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZX19010.1"
                     /translation="MKLVTAIIKPFKLDDVRESLSEIGVQGITVTEVKGFG"
BASE COUNT         1171 a         1785 c         1939 g         1151 t
ORIGIN      
        1 accagggcgt gctctatctg gttagcgagc cgaatctgtt ctatcgcttc aagaaaccag
       61 gcgcttaggc tgtggcgtga aggctgcggc tgttggttgc aacagacgcg cggttttcca
      121 ttcgaagccc agggtcaggc cgatggcggc acaggccagg ccgccggcca gcccccacca
      181 aacaccctcg gcgccccagc ccagcggaaa tgccagcgcc caggccagcg gtgcgccgac
      241 tgcccaatag cagccgaggc cgacccagaa ggtggtcttg gcgtccttga ggccgcgaat
      301 cgcgcccatg gcgatggtct ggatgccgtc gaagaattcg aaccaggcgg caatcgccag
      361 cagggccacg gctagggtta ccacctcggc ataggcgctg tccgtgtggt cgagaaacag
      421 cccgaccacc caatgcggtg ccagccagaa caggcaggca aaaccgagca tgcacagcgc
      481 gccgaggccc agacccaggc gaccggcttg gcgtgcctgc agcaggcggc cggcaccaaa
      541 gtgctgaccg atacggaagg tcactgcgta ggaaatccct accggcacca taaacgccac
      601 gtacaccgac atgatcgcga tctggtgcgc cgccagttgc gtgctgccca gtgcgcccat
      661 gcacagcgcg gcaaaggcaa acaggccgga ctccaccgca taggtgccgc caatcggcag
      721 gcccaggcgc agcagctctt tcagctctgc ccagactggc cgcagcagac cgcgccatag
      781 cgggtaaggg ccataggcgc tgtggtgcag cacatgccag gccagcagca gcgccatgac
      841 attcatcacc acggcggtga ccagaccgat tccggccagg cccagcgacg gcaggccgaa
      901 ccagcccttg atcaaggcat agttgaggac gaagttggcc agtgccccgc caatgcttat
      961 agccattacc gggccagggc ggccgatggc actggtaaag ccacgcagcg ccataaaact
     1021 catatagccg ggcagggcaa acaccagggt ggaaagaaac tgcataccgc cgctgacgtt
     1081 ctccggtttc tgcccgaaat acagcagagc cggcctcagg ttccacagtg ccaaaccggc
     1141 cagcaacgcc aggccccagc caagccatag gccgttttgc gtcagccggg tgacgcctgc
     1201 agcgtcattt gcgccatgac ggatcgccac cagattgccg accgaggcaa tcactccgac
     1261 acagaagatc gacacaaagg aatagctggc cgcacccagg ccgccggcgg ccaaggtcgc
     1321 ggggccaagc aggcccatca tcactgtgtc ggtaaacacc atcagcacat gcgccagctg
     1381 tgcggcaatc aagggtccag cgagtttcag gatagcgcgc agttcgtcgc gaagcggatg
     1441 cagcatgatg agttctactc agttagaagg cttgctcggg tgatccgcgc aggcagagaa
     1501 tgcgcctatt ctcataagcc ggccttggta gcacaaaagg ataatagcga tgatagacat
     1561 gactattact catggattgc agttgtgagc cagcgcttgc caccgctcta tgccctgcgc
     1621 gctttcgaag ctgcggcgcg gcatagttcc tttacccgcg ccggcgagga gctggcgatt
     1681 acccagagtg cggtcagccg gcatgtgcgc accctggagg aacacttcgc ctgccgtctg
     1741 ttcgagcgcc ggggccgcag cctgcagcta accgaaaccg cgcgtatgct gctgccgggg
     1801 ctgcgcgatg gctttgaggc gctggagcgt gcctgcaaca ccctgcgcgt cgacgacacg
     1861 gtactccgcc tgaaagcccc gtcgacgctg accatgcgtt ggttgctggc gcgcctgtcg
     1921 cgctatcgcc tgctcagcgc cagtgaggtg caactgacca gcgcctggat ggacgtggac
     1981 aaggtggact ttctgcatga gcctttcgac tgcgccgtcc tgctcagcca gggtgacttc
     2041 ccggaggaat gggaaagcac cgagttgttc gccgaatggc tgattccggt gtgcgcgcct
     2101 gaagccttga gcgaacaacc ctgggatctt ccgcgtttgc agaccgctga actgctgcac
     2161 ccgaccccgg atcgccgcga ctggcggcaa tggctgcagg ccatgggcct ggcggatcag
     2221 gtgccgctca agggcggcca ggtgttcgac accctggagt tgggcatggt ggcggcggcg
     2281 cgtggctatg gtgtgtcgat cggcgatctg gtgatggttg ccgaggatgt cgcccaaggc
     2341 cgactcagtc tgccctggcc cagcgcggtg cccagtggtt gtagctacta cctggtatgg
     2401 cccaaggcgc gccgaggcca ggagcgcttt cgccgtttgc gcgattattt agtggccgat
     2461 gtggcggcga tgcagctgcc cgtggtcgag cgggtgggca atcgggcgcg ttagcacggt
     2521 agcgcataaa gcgtaaaaaa tattggacta tcgtttagcg gtattatcag attaatactt
     2581 ttaaaacaga agcttacttc atttgttggg ctggggttct tgcggcggac ggtctgattt
     2641 cttaacgatt cgcgcttatt tgtttgacat attttacggc tttggcagac tggcttcagg
     2701 gttgtgactg ccagatgagc gtttctagcg catctgatgc cgctgcggcg ggcatctaga
     2761 caggcgcaat cagccgtaaa acagaaggtg cgtgacagcc gtcagccctt ctgcacaaca
     2821 acggcagcca atccgggctg ccccaaggtg atgtatgtcc aacaacaata cgaaacagcg
     2881 cccaggcgcg cgtaaaccgg ttgtcctgat gtccatgggc gctcaagagc gcaaagggca
     2941 tgcctatcaa gtcatgaccc acaaatacat aacccccctc gtcgaactgt ctggttgtgt
     3001 gccgctgctg gcgcccagct gctgcggtgc cgatgacatc gagcagtact tgtcgatggt
     3061 cgatggcgtg tacctgaccg gtgccggcag caatatcgac ccggccctct acggccagga
     3121 aaacctgacc ccgagtaagg gccaggaccc ggaccgcgat ctgttcgata tcccactgat
     3181 tcgcggcgca ctggcccgtg gcctgccgct gtttggtatc tgccgcggca tgcaggaaat
     3241 caacgtggcc ctgggtggca acatccacca gaaggtttac gaagtaccgg gcatgaatga
     3301 ccaccgcgaa gacaccagcg cgccggtgga tgtgcaatac gccgacagcc acagtgtgcg
     3361 cgcattgccg ggcagctggt ttgctgagct gatgggccat gcggatttcc cggttaactc
     3421 gctgcacggc caggccgttg atcaactggg cgagggaatc gaagctctgg ctcacgccga
     3481 agatggttta atcgaggcga ttcatctgcc gggcgccgag caattcacct tggcggtgca
     3541 atggcaccct gagtggcagg cggcgcagaa cccgcactcg gtgaaaatct tcaaggcgtt
     3601 cggtgatgcc tgtcgtgagc aggctgcccg ccgcgactcg caataactct acgtctagat
     3661 atataagaag ccccgcacgc ggggcttctt aatgggtgct attcaggcgc tgctgctggg
     3721 gcggtactgc agggcttcgc ccaggtggct gcggctgata tgtgcggcgc tgtccaggtc
     3781 ggccagggtg cgtgccactt ttaatacccg atgtgccgcg cgcaagctca ggcccaagcg
     3841 ctcgcaggcg ttttccagcc actggcggtc ttcgctttgc aggctgcaat gctgttgcac
     3901 gccgttgaga tccagatggg cattggcgca gccctggcga gtcagttgct gctggcgcgc
     3961 ctgcaccacc tgggcggcca gttcggcgct gcttggaccg ggttcggcgt tggcgtcaag
     4021 ccgggtagtt tcccgtggca cgctgagatg caggtcgatg cggtcgagca gcgggccgga
     4081 gagtttgccg cggtagcgct ggatctgctc gctgctacaa cgacagcgcc cggacggatc
     4141 gccaagatgc ccacatgggc agggattcat cgctgccacc agttgaaagc gcgcggggaa
     4201 acgtactttg tcgcgggcgc gagcaatcac gatctgccca ctttctaggg gctcgcgtaa
     4261 gacctccaag actttacgat caaattcggg tagctcatcg aggaataaaa cgccgtgatg
     4321 ggccaaggtt atttcgccag gttgcgggcg actgccaccg ccgaccagtg ccgggccgga
     4381 tgcactgtga tgaggcgttc taaaaggtct ttgcggccag gcactcagcg gcgtgtggct
     4441 ggccacggaa tgaatcgccg ctacttccag tgcttccgcc tcgtccagcg gtggcagtag
     4501 gcccggcagg cggctggcca gcagggtttt gccggtaccg ggtggtccag taaatagcag
     4561 attatgtgag ccggccgctg ctaccaatag cgcgcgtttg gcggcaatct gaccctgtac
     4621 ttcggccaga tcaggatagg gcagaacttg gcgcagcagg ccttgagcct gatacggcgc
     4681 aatcggcgcc tggccgttga ggtgggcggc gacttccaat aaatgctcga cggctattac
     4741 cgtcagcccc gaagccaggc tggcttcttc ggcattggcc ttgggcacca ctaaggtgcg
     4801 tccggcggcg cgagcggcca gtgcagcagg taatacgccc tgtactgggc gaatcgtccc
     4861 ggagagcgcc agttcgccca ggcattcgag gtgctccagc gcgctggctg gcacctgacc
     4921 gctggcggcg agaatgccga gggcaatcgc caggtcgaaa cgcccaccat ccttgggtaa
     4981 atcagcaggc gccagattta aagtgatgcg tctgggcgga aaatcgaagg cgcagttgag
     5041 aatggcgctg cgcacgcggt ccttgctttc cttgacggcg gtttccggca ggccgaccag
     5101 tgccagcgag ggcaggccat tggccaaatg ggcttcaacg gtgacagcgg gggcttcaac
     5161 tcccacctgg gcgcggctgt ggactatggc cagggacatc gatcactcct tgatcggctg
     5221 atgcatccgc agtgagcgtt actcgctggt ggttggggtg tctgtgctgc gcgcttccat
     5281 ctcggccact ttggcctcta acgcttccag gcgggcgcgg gtgcgggcga gcacgaccat
     5341 ctggctgtcg aactcgtcgc ggctgaccag gtcgagcttg ctgaaggcgc tttgcagcag
     5401 caccttgagc tgtgcttcga actcaccgcg cggcaagggc gcgtcgccat tgaacaggcg
     5461 cgaagcatgg gtggtcaggg catctagcag ggctttaggt ggcagcatgg tgaggttccg
     5521 tttgcagatg gcggcagtgt agcacgcagc tttactcgct ttaccgaggg cggctggcgc
     5581 gggatgggcg cttactgcac agttttcgcg cgctgcttgg caggctactg caccgttgtt
     5641 gtgcgcttac tgtgggctgt tatcaggcta aaacgcctgt ttgccgggta actgcatgaa
     5701 ataaatcgct tttgcctgat ctggcaagct ttctgctttg tcacttgcga cgcatgcacc
     5761 gatgcagttc cggtgcgtca ggcgaggcgg gaagtgtttc gtcgggagcg gttggtgagc
     5821 atcggtgccg ctgggtcagg gctgcgatgc attacaaaag ccaggcactg cgcttagact
     5881 tggaacgggt tcgtaattcc tggggcaagt ccaccataca cgggagaaag ttacatgaag
     5941 ctagtcactg ccatcatcaa gccgttcaag ctggacgacg tccgtgagtc actgtcggag
     6001 atcggcgtgc agggcatcac tgtcactgaa gtcaaaggct ttggtc
//
LOCUS       DASRUM010000290         3606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1980718, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000290 DASRUM010000000
VERSION     DASRUM010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1980718"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..85)
                     /locus_tag="VFF22_19350"
     CDS             complement(<1..85)
                     /locus_tag="VFF22_19350"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HZX19011.1"
                     /translation="MGKNVVVLGTQWGDEGKGKIVDLLTEQA"
     gene            complement(150..1337)
                     /locus_tag="VFF22_19355"
     CDS             complement(150..1337)
                     /locus_tag="VFF22_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HZX19012.1"
                     /translation="MATVDRWLLPDGIEEVLPPYAGRIEVARRQVLDLFQRWGYEFVV
                     TPHIEYLESLLTGAGQDLDLRTFKVTDPLSGRQMGFRADITPQVARIDAHTLRREGPS
                     RLCYAGSVVHAQPRALTTSRSPIQLGAELYGDASPASDVEVISLLVNTLELAAVPDVH
                     MDLGHVGIYRGLARAAELSGEVEQQLFDALQRKAMDEVAELTETLPDDLRKMLRALSE
                     LCGGREVLDLAQACLVDAPDDVHAALDDLMAIADALSLRYPELPLYFDLGELRGYHYH
                     TGVVFAAFVPGVGYAIAQGGRYDDIGADFGRARPATGFSTDLKTLVSLGQMQLGEEPA
                     GIWAPDNHDVFLWQAIVRLRQDGQRVVQALPGQVAADAEAAGCDRQLLLRDGRWQLAA
                     LTA"
     gene            complement(1375..1560)
                     /locus_tag="VFF22_19360"
     CDS             complement(1375..1560)
                     /locus_tag="VFF22_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HZX19013.1"
                     /translation="MWQELGIALCLVLVLEGILPFLCPRHWRGALLQLTQLSDRQLRL
                     LGLASMLLGTTVLYWLH"
     gene            complement(1661..2530)
                     /gene="hflC"
                     /locus_tag="VFF22_19365"
     CDS             complement(1661..2530)
                     /gene="hflC"
                     /locus_tag="VFF22_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HZX19014.1"
                     /translation="MSNKSLIALIVGVVLALVAWNSFYIVSQTERAVLLQFGRIVQPD
                     VQPGLHVKIPYVNQVRKFDARLLTLDSTSSRFLTLEKKALMVDAFAKWRVLDAERFYT
                     ATSGMKQIADERLARRLEASLRDQFGKRTLHESVSGERDALMADVTASLNRAAQKELG
                     IEVVDVRVKAIDLPKEVNRSVFDRMSSEREREAREHRAKGKELAEGIRADADRQRRVL
                     LADAYRQAEELRGEGDAQAAAIYSKAYGMDQEFYSFYRSLRAYRESFADKRDVLVLDP
                     SSDFFRYLEKSKP"
     gene            complement(2530..>3606)
                     /gene="hflK"
                     /locus_tag="VFF22_19370"
     CDS             complement(2530..>3606)
                     /gene="hflK"
                     /locus_tag="VFF22_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HZX19015.1"
                     /translation="PDIDEAFRKLQESLNGLFGGGKKRGSETGSGNGGGFGLLFVGLG
                     LLAAVWLYSAIYVVDEQEQAVVLRFGKYYETVGPGLNIYFPPIDRKFQENVTRERAYS
                     KQGQMLTEDENIIEVPLTVQYRVSNLQDFVLNVDQPEVSLQHATDSAVRHVVGSTEMD
                     QVLTEGRELMASEVKDRLQRFLDNYRTGITVTQVNLQSAAAPREVQEAFDDVIRARED
                     EQREKNQAETYANGVVPEARGQAQRLIEDASGYRDEVVSRAQGEADRFTALVTEYRKA
                     PEVTRERLYLDTMQELMSNTSKVLVTGDKGQNNLLYLPLDKMIDGRSSSASSAVSGAA
                     GTADAGSRVTIDPRQVELRTRESR"
BASE COUNT          778 a         1215 c          952 g          661 t
ORIGIN      
        1 cagcctgttc ggtcagcagg tcgacgatct tgcccttgcc ttcatcgccc cactgggtgc
       61 ccaggactac gacattctta cccataacac taaccctctt cgcaaacttg atgccggcca
      121 tagccgacgg aaacttttcg cacgaccact caggctgtca gcgcagccaa ctgccagcgg
      181 ccatcacgca acagcagctg acgatcacag cctgcagcct cggcgtcagc cgcgacctgc
      241 ccaggcagcg cctgaactac gcgctgaccg tcctggcgca agcgcacaat tgcctgccac
      301 aaaaacacat catgattatc cggagcccag atacctgcag gctcttcacc caactgcatc
      361 tgcccaaggc ttaccagggt tttcaggtcg gtggagaagc ctgtcgccgg acgcgcccgg
      421 ccaaaatcgg cgccgatgtc gtcataacgg ccgccctggg cgatggcata acccacaccc
      481 gggacaaagg ccgcgaatac cacaccagtg tggtagtgat agccgcgcag ctcgcccaga
      541 tcgaagtaca gcggtaattc cgggtagcgc aagctcagcg catcggcaat cgccatcaag
      601 tcatccagcg ccgcatgcac atcatccggc gcatcgacca gacaagcctg ggccagatcc
      661 aatacctcac gaccaccaca cagctcgctg agcgcgcgca gcatcttgcg caggtcatcc
      721 ggcagggttt cggtcagctc ggcaacctca tccatcgcct tgcgctgcag cgcatcgaac
      781 aactgctgtt caacctcgcc agacagctcg gcggcgcgcg ccagaccgcg atagatgccg
      841 acatggccaa gatccatgtg cacatccggc accgcagcca gctcaagggt gttgaccagc
      901 aggctgatca cttccacatc gctggccggg ctagcgtcgc catacagctc ggcgcccagc
      961 tgaatcgggc tgcgcgaggt ggtcagcgca cgcggctggg catgcaccac gctaccggcg
     1021 tagcacagac ggctcggccc ttcgcggcgc agggtatgcg catcaatgcg cgccacctgc
     1081 ggcgtgatgt cggcacgaaa gcccatctgc cggccagaca acggatcggt caccttgaag
     1141 gtgcgcagat ccaggtcctg gcccgcgcca gttagcagcg attccaggta ctcgatatgc
     1201 ggggtgacca caaattcata gccccagcgc tggaacagat ccagcacctg gcggcgcgcc
     1261 acctcaatac gccctgcgta cggtggcagt acttcttcga tgccatctgg caggagccag
     1321 cggtctaccg ttgccatttc gccattcccc tcttgaaccg ggcggcacaa gccttcagtg
     1381 aagccaataa aggacggttg ttcccagcag catgctggcc aaccccagca gacgcagctg
     1441 cctgtcagac aactgcgtca gttgtaaaag tgcgccacgc caatggcgcg gacacaggaa
     1501 gggcaggatg ccttccagca ccagcacgag acacaacgcg atgcctaact cctgccacat
     1561 gcttcccaca gacgcaaaaa agccgggatt ttcccggctg ccgcatgata ccacgttttc
     1621 cacaaggatc accccggcgg ctgcccgccg gggtgatgac ttaaggcttg gacttctcca
     1681 gatagcggaa gaagtcgctg ctcggatcca gcaccagcac gtcacgctta tctgcgaagc
     1741 tctcgcgata agccctcagg ctgcgataga acgaatagaa ctcctggtcc atgccatatg
     1801 ccttggagta gatcgcagca gcctgggcat caccctcacc gcgcagctct tcagcctgac
     1861 ggtaggcatc tgcgagcaac acgcgacgct gacggtcggc gtcggcgcga ataccttcgg
     1921 ccagctcctt acccttagcg cggtgttcac gagcctcacg ctcacgctcg gaactcatgc
     1981 gatcgaatac gctgcggtta acttccttcg gcagatcgat ggccttgacg cgcacatcca
     2041 ccacctcgat gcccagctct ttctgcgcag cacgattcag cgatgcagtc acatccgcca
     2101 tcagcgcatc acgctcaccg gacaccgact catgcagagt gcgcttaccg aactggtcac
     2161 gcagcgacgc ttcaagacga cgcgccagac gctcgtcggc aatctgcttc ataccggaag
     2221 tcgcggtgta gaaacgctcg gcatccagca ctcgccattt ggcgaaggca tcaaccatca
     2281 gcgctttctt ttccagcgtg aggaaacgcg agctggtgga gtccagggtc agcaaacgcg
     2341 catcgaactt gcgcacctgg ttgacgtacg gaatcttcac atgcaggccc ggctgtacgt
     2401 ccggctggac gatacgacca aactgcaaca gcaccgcacg ctcagtctgc gacacgatat
     2461 agaagctatt ccaggcgacc agcgccaaca ccacgccaac aataagggcg atcagcgatt
     2521 tattgctcat caacggctct cccttgtacg cagttcaacc tgacgcggat caatggttac
     2581 ccgcgagccg gcgtctgcag tacccgcagc gccagatacc gcgctagacg cagacgagct
     2641 gcggccatca atcatcttgt ccagcggcag ataaagcagg ttgttctgcc ccttgtcgcc
     2701 agtcaccagc accttgctgg tattgctcat cagctcttgc atggtgtcca ggtacagacg
     2761 ctcgcgagtg acctcgggcg ccttgcggta ctcggttacc aatgcagtaa agcgatccgc
     2821 ctcaccctgg gcgcgggaaa ccacttcatc acggtaaccg ctggcgtcct caatcagacg
     2881 ctgcgcctga ccacgagctt ccggcaccac accattggcg taggtttccg cctgattctt
     2941 ctcacgctgc tcgtcttcac gggcgcggat cacgtcatcg aaggcttcct gaacttcacg
     3001 cggcgccgca gcactctgca ggttgacctg agtaacagtg ataccggtac ggtagttgtc
     3061 gaggaaacgc tgcaagcgat ccttgacctc actggccatc agctcacggc cttcggtcag
     3121 cacctggtcc atctcagtgg aacccaccac gtgacgcacg gcactgtcgg tcgcatgctg
     3181 cagactgact tccggctgat cgacgttgag gacgaaatcc tgcaaattgc tgacccggta
     3241 ctgcacggtc aacggcactt cgatgatgtt ttcgtcttcg gtcaacatct gcccctgctt
     3301 gctgtaagca cgctcgcggg tgacgttttc ctgaaacttg cgatcaatcg gcgggaaata
     3361 gatgttcaag cccggaccga ctgtttcgta atatttgccg aagcgcagca ccacggcctg
     3421 ctcctgctca tcaacgacat agatcgcgct gtacagccag actgcagcca acagccccag
     3481 gcccacgaac agcaagccga agccgccgcc attgccagag ccagtctcac tgcctcgttt
     3541 cttgccgcca ccgaacagcc cattaaggct ctcctgcagc ttgcggaagg cctcatcgat
     3601 atccgg
//
LOCUS       DASRUM010000291         4496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2317978, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000291 DASRUM010000000
VERSION     DASRUM010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2317978"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..2083
                     /locus_tag="VFF22_19375"
     CDS             80..2083
                     /locus_tag="VFF22_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HZX19016.1"
                     /translation="MSHDNDNSVLFGNGDELPSNASSNPHINQLIDGVGRRQVLAAGA
                     ALGTLAFLGSVMPGAAVAAEPAAKGLDKLHWFKPRSKLPFTAVATNRLDNISVPAGYR
                     ATTFIPWGTPITGNYPAYLGDGSNSAQDQAEQLGMHHDGMHFFPIDAQRGGKKSDHGL
                     LVLNHEYIDAPLLHPNGPTLVAGKRSSAEEVRKEINAHGVSVVEVRRGLNGEWAVLPS
                     ARNRRITGATPMQISGPARGHVLLKTRYSPKGTATRGTLNNCSHGFTPWGTYLTCEEN
                     WAGYFASRDTDLPRELSRYGLRSSSRYGWDHLKGDEFERFDATRITTTASADYRNEPN
                     HFGWIVEIDPFAPESVPVKRTALGRFAHEGLVFAPVKSGRPVVCYSGDDSQNEYIYKY
                     VSRDKYRPGRSNGRLLDEGTLYVARFDSSGKGEWLALDINDKKFQKACKAAGVNFADQ
                     GEVLINTRLAADVVGATKMDRPEWGAVNPDNGEVYFTLTNNSARTSADAANPRPANAF
                     GHIIRWRELSKDYAGRQFAWSLFMLAGPKTDSIGPNGKPLTADNRLASPDGLWFDEEG
                     RLWIQTDMSGSQLSSGPFGNNQMLVAEPRTGELKRFLVGPVGAEVTGITATPAFRTLF
                     VNIQHPGEGSTATNLLSTWPDGPGQRPRSATVIITREDGRRLL"
     gene            2175..2663
                     /locus_tag="VFF22_19380"
     CDS             2175..2663
                     /locus_tag="VFF22_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012075445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HZX19017.1"
                     /translation="MSSNRLSRIVGKIQQLPSALQSPALSLLFGSQVKFAGTAKVRVH
                     TLTQQQAVMSIANKRKVQNHIKGVHAAAMALLAESATGFLVGMNVPDDKLPLIKSLKV
                     DYLKRATGSLRAVASLTPEQIQAIHTQEKGEVLVAVTVTDEAGIQPIQCEMLWAWVSK
                     KR"
     gene            complement(2769..4451)
                     /locus_tag="VFF22_19385"
     CDS             complement(2769..4451)
                     /locus_tag="VFF22_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX19018.1"
                     /translation="MRLTLKAKVVLLALIPVVLFALVLSGAAAKVLQNLAAQEIEETR
                     ERLLQESRNELQHYVQIALGSVQTLYDGAAQGDMDSREQAIVILSKIKFGKDGYFFGH
                     DSNVVRLFRGDSPVDVGKSLVDRRDPNGVYTNRELVNVAKNNTYYVNYSSPLPTDDSV
                     MVPKMAYSYYLPKWDMALGTAINLDGVEAQIAQVQGEIDERIGTIITSIMVIAAVLLV
                     IFGIVGVVLSNTFLRPLQQIKDNLDDIAAGEGDLTRRLPINGDDELGQLAGSFNRFVE
                     KIHGLVRQIVDMTGQLTELVGQVSAQAQRSEEAMERQRHETDQVATAINEMSAAAHEV
                     AQSAQGAAEAAQKTDTEGQAAKKVVDGSIERIHTLVQDIRDSGVSLDTLQKDVSSIVS
                     VLDVIRSIAEQTNLLALNAAIEAARAGEAGRGFAVVADEVRALASRTQQSTQEIQGMI
                     DRLQKGTQDAVTAMRRSSDAGDVTSEQANRAGTSLDAIAQLIGTINAMNAQIASAAEE
                     QTAVAEEINRSVHKIAVAVDSVADETQQGAQTARNLAGLGERLGALVRQFRI"
BASE COUNT          907 a         1426 c         1305 g          858 t
ORIGIN      
        1 tttcgcacag ccgctgtcat gtttacgtca atttcattgg atagcgtcag gcacccgtcg
       61 attaatccaa ggagcctgca tgagccacga taatgacaac tcagtgctgt tcggcaatgg
      121 cgatgaattg cccagcaacg cgtccagcaa cccgcatatt aatcagctga tcgacggcgt
      181 cggccgtcga caagtgttgg ccgccggtgc cgcgcttggc accctggcgt ttctcggcag
      241 tgttatgcct ggcgccgcag ttgcggcaga gcccgccgcc aagggcctgg acaagctgca
      301 ctggtttaag ccacgcagca aactgccgtt caccgcagtc gccaccaacc gtttagacaa
      361 catcagcgta ccggcaggtt accgcgccac cacctttatc ccctggggca cacctatcac
      421 cggcaactac cctgcttacc tgggcgatgg cagcaacagc gcccaggatc aggccgagca
      481 actgggcatg caccatgacg gcatgcattt cttcccgatt gacgctcagc gtggaggcaa
      541 gaagagtgat cacggcttgc tggtgctcaa ccacgaatac atcgacgcgc cgctgctgca
      601 ccccaatggc ccgacgctgg tggccggcaa acgcagcagc gccgaagaag tgcgcaagga
      661 aatcaacgcc cacggcgttt ccgtggtgga agtacgtcgt ggcctgaatg gcgaatgggc
      721 ggtattgccg agcgccagaa accgccggat tactggtgcc acacccatgc agatcagcgg
      781 tccggcgcgc ggccatgtgt tgcttaaaac ccgttacagc ccgaaaggca cggccactcg
      841 cggcaccctg aacaattgct cacacggctt cacgccttgg ggcacttacc tgacctgtga
      901 agagaactgg gccggctact ttgccagccg cgataccgac ctgccgcgcg agctgagtcg
      961 ctatggcctg cgcagtagca gccgctacgg ctgggatcac ctcaagggtg atgagttcga
     1021 gcgcttcgat gccacgcgca tcaccaccac tgccagcgcg gactatcgca acgagcccaa
     1081 ccactttggc tggatcgtcg aaatcgatcc attcgcgccg gagtcggttc cagtcaaacg
     1141 caccgccctc ggtcgcttcg cccacgaagg cctagtgttc gctccggtga aatcgggccg
     1201 tccagtggtg tgctactccg gcgacgactc gcagaacgaa tacatctaca aatacgtcag
     1261 ccgcgacaaa taccggccag ggcgtagcaa tggccggctg ctggacgaag gcacgctgta
     1321 tgttgcgcgc tttgatagca gcggcaaggg tgaatggctc gcgctagata tcaacgacaa
     1381 gaagttccag aaagcctgca aggccgccgg ggtgaatttt gccgaccagg gtgaggtgct
     1441 gatcaatacc cgcctagccg ctgacgtggt cggcgcgaca aagatggacc ggccggaatg
     1501 gggcgcggtc aatccggaca acggcgaggt gtacttcacc ctgaccaaca acagtgcccg
     1561 aacaagtgcc gatgccgcca acccgcgccc agccaatgcg ttcggccata tcatccgttg
     1621 gcgtgagctg agcaaggatt acgccggccg ccagtttgcc tggagcctgt ttatgctggc
     1681 cggcccgaag accgacagca tcggcccgaa cggcaaaccg ctgactgctg ataatcgcct
     1741 ggccagcccc gacgggctgt ggttcgacga ggaaggccgc ctgtggattc aaaccgacat
     1801 gagcggcagc caattgagca gtggtccatt cggcaacaac cagatgctgg ttgccgagcc
     1861 gcgtaccggt gagctgaaac gtttcctggt ggggccagtg ggcgccgagg taacgggcat
     1921 taccgcaacc ccggccttcc gcaccctgtt cgtcaatatc cagcatccgg gcgaaggctc
     1981 aacggcgacc aacctgctca gcacctggcc cgatggcccc ggtcagcgtc cgcgctcggc
     2041 caccgtgatc atcacccgcg aagacggccg tcgattgctc taatcgacgg gtaatcatgt
     2101 gaccggtcag ccctggccgg tcacatttcg ctgtgacaga ctcgtgccct tccctttgcg
     2161 caaagagcat cagcatgtcc agcaaccgct tgagccgtat cgtcggcaaa atccagcaac
     2221 tgcccagcgc cctgcaaagc ccggcgctgt cgctgctgtt tggctcccag gtgaagttcg
     2281 ccggcaccgc caaggtacgc gtgcatacgc tgacccaaca gcaggcagtg atgagcatcg
     2341 ccaacaagcg caaagtacag aaccacatca aaggcgtgca tgccgccgcc atggcgctgc
     2401 tggctgaatc cgccacgggc tttctggtcg gcatgaatgt gcccgatgac aaactgccgc
     2461 tgatcaaaag cctcaaggtc gactacctca agcgcgccac cggcagcctg cgcgccgtgg
     2521 caagcctgac acccgagcag attcaagcca tccacaccca ggaaaaaggc gaagtactgg
     2581 ttgccgtgac cgtcaccgat gaagccggta tccagccgat ccagtgcgaa atgctttggg
     2641 cctgggtcag taagaagcgc tgagtgcagc caacctctgg cgcgagctga aacctccaat
     2701 gctggaatag tgccttggct agccaaagaa gggacgcaat gcgtcccttc ttccatgctc
     2761 tagctgagtt agatgcggaa ctggcgcacc agagccccca agcgttcgcc cagcccagcc
     2821 aggttgcgcg cggtctgtgc gccctgctgg gtttcgtcgg ccacactgtc taccgccacg
     2881 gcaatcttgt gcacgctgcg gttgatctct tcagccactg cagtctgctc ttcggcggcg
     2941 ctggcgatct gcgcgttcat cgcgttgatg gtgccgatca gctgcgcgat ggcatccagc
     3001 gacgtaccgg cgcggttggc ctgttcgctg gtgacatcgc cggcatcact ggaacggcgc
     3061 atcgccgtca ccgcatcctg ggtgcccttc tgcaagcggt cgatcatccc ctgaatttcc
     3121 tgggtgctct gctgcgtacg gctggccaac gcccgcactt catcggccac cacggcaaag
     3181 ccgcgccccg cttcaccggc ccgcgcagcc tcaatggctg cgttgagagc cagcaggttg
     3241 gtctgttcgg cgatggagcg gatcacgtcg agcacactga caatcgacga cacgtctttc
     3301 tgcagagtgt cgagggacac gccgctgtcg cggatgtcct gcaccagggt gtggatgcgc
     3361 tcgatgctgc catcgaccac ttttttcgcg gcctggcctt cagtgtcggt cttctgcgcc
     3421 gcctctgcag cgccctgggc gctttgcgcg acttcatggg cagcggcgga catttcattg
     3481 attgcggtcg caacctgatc agtctcatgg cgctggcgct ccatggcctc ttccgaacgc
     3541 tgcgcctggg ccgagacctg gccgaccagt tcggtcagct ggccggtcat gtcgacgatc
     3601 tgccgtacca ggccgtggat cttctcgaca aagcggttga acgagccggc cagttggcca
     3661 agctcatcgt cgccattgat cggcaggcga cgagtcaggt cgccttcacc ggcggcgatg
     3721 tcatcgaggt tgtccttgat ctgctgcaac gggcgcagga aggtattgct cagcaccaca
     3781 ccgacaatac cgaatatcac caacagcaca gcggcaatca ccatgatgct ggtgataatg
     3841 gtgccgatgc gctcgtcgat ttcaccttgc acttgggcaa tttgcgcctc gacgccgtcg
     3901 aggttgatcg ccgtgcccaa ggccatgtcc catttcggta ggtaatagct gtaggccatt
     3961 ttcggcacca ttaccgaatc atcggtcggc agtggcgagc tgtaattgac gtagtaggtg
     4021 ttgttcttgg cgacattcac cagttcacgg ttggtgtaca ccccattcgg gtcgcggcga
     4081 tcgaccagac tcttgccgac atccaccggg ctgtcgccgc gaaacaagcg aaccacattg
     4141 gagtcgtggc cgaagaagta gccatccttg ccaaacttga ttttcgaaag gataacaatg
     4201 gcctgctcac gactgtccat atcgccttgc gcggcaccgt catacaaggt ctgcaccgaa
     4261 ccgagggcga tctgcacgta atgctgcagc tcgtttcggc tttcctgcag caggcgctcg
     4321 cgggtttcct cgatctcctg ggccgccagg ttttgcagta ccttggccgc ggcgccactg
     4381 agtaccaggg caaacagcac cacaggtatc aatgccagca gcactacttt ggctttcaat
     4441 gtaagacgca tcgggcagat ccttgcttgg cagttacagc gaaataaaaa cggggc
//
LOCUS       DASRUM010000292        14733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_323867, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000292 DASRUM010000000
VERSION     DASRUM010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 14733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14733
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323867"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VFF22_19390"
     CDS             <1..326
                     /locus_tag="VFF22_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HZX19019.1"
                     /translation="MPIREIWDMQERLPRRSGKRADMLLENPRFRAGYDFLLLRESAG
                     EETGGLGDWWTRYQDVSDSERRNMIRDLSSKDDGPAGPRKRKRSNSKRKRGPGSDAGS
                     PAASE"
     gene            326..820
                     /gene="folK"
                     /locus_tag="VFF22_19395"
     CDS             326..820
                     /gene="folK"
                     /locus_tag="VFF22_19395"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HZX19020.1"
                     /translation="MERVYIGLGSNLATPLAQLRSALPALAALPHTRLIAQSSFYASD
                     PLGPADQPRYVNAVAALDTDLSPLELLDALQTIELEQGRTRKAERWGPRTLDLDILLF
                     GQRQLDEPRLSVPHYHMHARAFVLYPLAEIAPDLHLPDGRALGELLTACPFAGLERID
                     ESPL"
     gene            1110..1910
                     /gene="panB"
                     /locus_tag="VFF22_19400"
     CDS             1110..1910
                     /gene="panB"
                     /locus_tag="VFF22_19400"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HZX19021.1"
                     /translation="MPDVTLTTLHSLKQSGEKIAMLTCYDATFAQTACQAGVDVLLVG
                     DSLGMVLQGHDSTLPVTVAEMAYHTASVKRGNQGALILADLPFMAYATIEQTLNNSAA
                     LMQAGAHMVKLEGAAWLAEPIRQLAERGVPVCAHLGLTPQAVNILGGYKVQGRQESQA
                     RQMRADAMALEQAGAAMLLLECVPSELAAEISQAVKIPVIGIGAGGATDGQVLVLHDM
                     LGLSLSGRTPKFVKNFMAGQSSIQHAISAYVKAVKDQSFPAAEHGFSA"
     gene            1907..2767
                     /gene="panC"
                     /locus_tag="VFF22_19405"
     CDS             1907..2767
                     /gene="panC"
                     /locus_tag="VFF22_19405"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014340243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HZX19022.1"
                     /translation="MNTVKSVRELRAAVAQARAEGKQIAFVPTMGNLHAGHAALVEKA
                     AQRADFVVASIFVNPLQFGPSEDLDKYPRTLIADQEKLVEAGCHLLFTPDVEEIYPHG
                     MDGQTRVSVPGVSEGLCGASRPGHFEGVATVVSKLFNMVQPDLAIFGQKDFQQLAVIR
                     TLVRDLNMPIQIIGEPTVRAEDGLALSSRNGYLNAEQRAAAPTLYRSLEQIASAIQAG
                     ERDYPKLIAQAQQLQSAAGFRPDYLEIRESNSLRPASAEDRQLVILVAAFIGSTRLID
                     NLALELEPSA"
     gene            2893..4557
                     /gene="pgi"
                     /locus_tag="VFF22_19410"
     CDS             2893..4557
                     /gene="pgi"
                     /locus_tag="VFF22_19410"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012312664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HZX19023.1"
                     /translation="MAYYQHPHDVTQLPAWQALRQHREQMNDFSMREAFASDPQRFDH
                     FSLSSCGLLLDYSKNLANEKTLELLVQLAEQAGLSESIKAQFNGERVNASEGRAALHT
                     ALRSPIGKRLELDGVDLIPQVHRVLNQMTELVSRIHSGLWRGYSEKPITEVVNIGIGG
                     SFLGPQLVSEALRPFTQRGVRCHYLANIDGSEFRDLTARLNPETTLFIVSSKSFGTLE
                     TLKNTLAARDWYLAMGGPEAELHKHFIAVTSNQQAAIEFGIKAENIFPMWDWVGGRYS
                     LWSAIGLPIALAIGISNFKELLAGAYAMDQHFAQAPLAQNMPVLMALLGIWYGNFWDA
                     KSQAILPYDHYLRNFTKHLQQLDMESNGKSVRQDGSPVDFNTGPVIWGGVGCNGQHAY
                     HQLLHQGTQLIPADFIVPVNSFSPLADHHQWLFANCLSQAQALMHGKTRAEAEAELRA
                     KGLDEAEVQRLAPHKEIPGNRPSNILALNRIDPFGLGALVALYEHKVFVQSAIWGINA
                     FDQWGVELGKELGQGVYQRLTGQLDTPASDASTQGLIQHFRERHRG"
     gene            complement(4564..5568)
                     /locus_tag="VFF22_19415"
     CDS             complement(4564..5568)
                     /locus_tag="VFF22_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HZX19024.1"
                     /translation="MRELPLPTSADLQDVLLLVFDNFQLLDASGPLEVFAVVNDRLAA
                     AGLPPRYRLRLVSQHGGEVRASAGVVVLTEPLPPIVQLAGATLLVAGGFGVMQVLAGG
                     QLAAWLRQAAEQLARCCSVCTGAFLLGQAGLLDGRMAVTHWRYVELLQRSFARIEVCA
                     DALFIKDGNCYSSAGVTAGIDLCLALVEEDCGSAMALDVAKDLVLPLRRSGGQRQFSA
                     ELLAQTGAPGGLAESLKQWLQPRLQQRLDNEAMAAGLSLSVRTLHRRCREELQMTPGH
                     YLQQLRLEAACRQLEQGARSLKRVAEQCGFVSLYNLRRCFSQRLGVTPDEYRQRFCRN
                     "
     gene            5693..6802
                     /locus_tag="VFF22_19420"
     CDS             5693..6802
                     /locus_tag="VFF22_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017518926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HZX19025.1"
                     /translation="MPTSLLLLSLCLSLLLDTTTARAATSPPISDEAIAAYVPRHGRA
                     RPVIAVLGENRMTELVDFVVPYGILKRADIAEVLAVAPEPGTITMLPALSFAADASTA
                     EFDARYPQGADYLIIAATHHNDDVRLLTWIRAQAGKGATMLGICDGVFSLAHAGLLNG
                     RRATGHWYSASERREQFPQTQWQEQRRYVVDGAMISSSGVSAAMPVSLALVEAIAGQL
                     RAQQLADELGLADWSAAHDSRAFTLHRADYLTLAGNWLAFWAHETLAIPVQVGIDEIA
                     LALTADAYARTYRSQVLSIATASLVTRSGLRLIPDRAHSKPGMRSLSIAKGPASHALQ
                     QALADIANRHGPATARLVALQLEYPSPYLGPESPP"
     gene            6881..8818
                     /gene="acs"
                     /locus_tag="VFF22_19425"
     CDS             6881..8818
                     /gene="acs"
                     /locus_tag="VFF22_19425"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HZX19026.1"
                     /translation="MFEITRHPVPDAVRQRAHLDNDDYLRLYQQSIEQPEIFWAEQAK
                     AFLDWSKPWTSVQQGDLLSGQGSWFKGGQLNVSYNCIDRHLEKRGEQIALIWEGDNPS
                     ESAHITYKKLHHNVSRLANVLKSRGVKKGDRVCIYMPMIPEATYAMLACTRIGAVHSV
                     VFGGFSPDALRDRILDSDCHTVITADEGVRGGKYVALKANVDKALQSCPAVSTVVVVE
                     RTQGEIDWVEGRDIWYHEALREVSGDCPPEPMDAEDPLFILYTSGSTGKPKGVLHSTG
                     GYLLMAAMTHKYVFDYHEGDIYWCTADVGWVTGHSYIVYGPLANGATSLIFEGVPNYP
                     SASRFWQVIDKHQVNIFYTAPTALRALMREGSGPVECTERTSLRLLGSVGEPINPEAW
                     EWYYNVVGERRCPIVDTWWQTETGAILITPLPGATDLKPGSATRPFFGVQPVLLDEQG
                     KEIDGPGSGVLAIKDSWPSQIRSVYGDHQRMIDTYLKPYPGYYFSGDGARRDEDGYYW
                     ITGRVDDVINVSGHRIGTAEVESALVLHDAVAEAAVVGYPHDLKGQGIYAFVTPMNGV
                     EINDALKKELLALVGKEIGSFAKPELIQWAPGLPKTRSGKIMRRILRKIACNELENMG
                     DTSTLADPTVVDGLIEQRLNR"
     gene            8871..9746
                     /locus_tag="VFF22_19430"
     CDS             8871..9746
                     /locus_tag="VFF22_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HZX19027.1"
                     /translation="MELLRRRIESQVLSLSGIALGQIDFEKPQGDPGLFGPDSVSWQV
                     HGDFTSMLIGGISALLLQALHPLALAGVWDHSNFREDLLGRLRRTGQFISGTTYGSRA
                     DADWLIDKVKTIHLKVSGTAPDGRAYAASDPALLTWVHVAEVHSFLQAHLRYRNPQLS
                     EADQDRYYAEIALIAERLGATQVPRSRAEIAAYLAAVRPELLCDERSLEILRILLGAP
                     PPSALAAPPAKLLMQAGIDLLPAWAQQMLGQQISPSRSRMIHAGVHSLAPVLRWAVRG
                     GAIHRARKRLQLPPR"
     gene            9842..11023
                     /locus_tag="VFF22_19435"
     CDS             9842..11023
                     /locus_tag="VFF22_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003211627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZX19028.1"
                     /translation="MQDVVIVAATRTAIGSFQGALANIPAPELGAAVIRRLLEQTGLD
                     GAQVDEVILGQVLTAGSGQNPARQAVILAGLPHAVPALTLNKVCGSGLKALHLGAQAI
                     RCGDAEVIIAGGMENMSLAPYVMPGARTGLRMGHAKLVDTMITDGLWDAFNDYHMGIT
                     AENLVDKYNISREEQDAFAAASQQKAVAAIEAGRFADEITPIMIPQRKGEPLAFATDE
                     QPRAGTTAESLGKLKPAFKKDGSVTAGNASSLNDGASAVLLMSAAKAQALGLPVLAKI
                     ASYANAGVDPAIMGIGPVSATQRCLAKAGWSIADLDLIEANEAFAAQALAVGKELGWD
                     TSKVNVNGGAIALGHPIGGSGCRVLVTLLHEMIKRDAKKGLATLCIGGGQGVALAIER
                     A"
     gene            11128..12144
                     /locus_tag="VFF22_19440"
     CDS             11128..12144
                     /locus_tag="VFF22_19440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZX19029.1"
                     /translation="MPSLDQALRAALHNRQDLLAKLHQQGTDCYRLFHGSQEGASGLT
                     IDRYGPQLLVQSFHQSLSRDELLQLAEQCQTSLGTQLLLVYNDRSQGNSRIDRTDTVY
                     QANPAALEDLVGHEWGLNYRVRGRHAGQDPLLFLDLRNARGWVKAHSAGKSVLNLFAY
                     TCGVGLSAAAGGANEVLNLDFAEGNLAVGRENGQLNPQLLPMQFVQSDYFPAIRQLAG
                     LPINARRGQKLPHYPRFEQRQFDLVLLDPPAWAKSAFGTVDLLRDYQSLLKPAILATA
                     DDGVLICCNNLAKVAMADWREQVLRCAEKLGRPVRDCQVLPPASDFPSQDGQPPLKTL
                     ILQF"
     gene            12247..>14733
                     /locus_tag="VFF22_19445"
     CDS             12247..>14733
                     /locus_tag="VFF22_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020293205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HZX19030.1"
                     /translation="MLKGIKRAASAIAIAVALYSLLGFLILPGIGLRIANQQLAQYAE
                     VPATLQRLQLNPFSLELSLWGLQIGEAEQQQIAFERLYANLQLDSLWSGVLHLADIEL
                     DKPHSEVLFGKDGRLNLTQLFKVPPSPQPAVEEPAGEPFPLRIARIKLSGGNVHFQDL
                     RPSEPIEFIYDDLNFELFNLSTLPDDNADMNLVATGPQGGRIDWTGRIGLVPISSNGS
                     LKVTDGKLKAIWPYVRDAVPLVLEDGTVNLSSDYSLNLAKGTELLLSNTQLSVSSFAI
                     KNPADKPLLRLESLDISETSVDLAKQQVTVGKIRSQKLETWAAREADGQLDWQKLLAS
                     QPAKPAPAPAPAPAPNEGGAGSAATEAEPVNDPTEPTTAEQSADLETATAGQPDEPSK
                     PWQVILRDVQLRDYQVHLTDRAGGSEVKVDLSPLNLDLSDFDSLGTSPFALKLDTGVN
                     KTGQIKADGQVQLTPTTAKLQVATRDIDLRLAQTYLSPFVRLELRSGKLGSDLAVDLQ
                     SVEPLAFSIGGQAEISQLHTLDTLKDRDFLKWQQVLVEGIDYQHGKGLVIGQVNLQQP
                     YVRFIINEDLTTNINDLMIPQPAEANAKPAAASKPLPMRIGGISIKDGSANFADFSLT
                     PNFATAIQQLNGQIGTLDNQSPKTASVDIQGKVDKYAPVSIKGKLTPFDPMNSLDIAT
                     SFKNVELTTLTPYSGKFAGFRIRKGRLNLDLHYQIEKGQLNAENKLLLEDLQLGEKVD
                     SKDAMDLPIRLAIALLKDTDGNISIQLPVQGDLNSPQFSVMPIVWQTLRNLVLRAAQA
                     PFKFIGGLVSGGSDVDLSTVRFNAGASELDG"
BASE COUNT         2987 a         5072 c         4284 g         2390 t
ORIGIN      
        1 ccatgccgat ccgcgaaatc tgggatatgc aggaacgcct gccacgccgc agcggcaaac
       61 gcgccgacat gctgctggaa aacccacgct tccgcgccgg ttacgatttc cttctgctgc
      121 gtgaaagcgc cggtgaagaa accggtggcc tgggcgactg gtggacccgc taccaggacg
      181 tcagcgacag cgaacggcgc aatatgatcc gcgacctcag cagcaaggac gatggccccg
      241 ccgggccacg caagcgcaaa cgcagcaaca gcaagcgcaa acgcggccct ggcagtgacg
      301 ccggcagccc cgccgcgagt gaatgatgga gcgcgtgtat atcggcctgg gtagtaacct
      361 tgccacaccg ctcgcccaac tgcgcagcgc cctgcccgcc ctggccgcgc tgccgcacac
      421 ccgtctgatc gcccagtcgt ccttctatgc cagcgaccca ctgggcccgg cggatcagcc
      481 gcgctacgta aatgccgtag ccgcactgga cactgatctc agccccctgg aactgcttga
      541 tgcgctgcaa accatcgaac tggaacaggg ccgcacgcgc aaggccgaac gttggggccc
      601 gcgcactctc gacctggata tcctgctgtt cggccaacgc cagctggatg aaccacgcct
      661 gagcgtgccg cactaccata tgcacgcccg cgcctttgtc ctctaccccc tggcagaaat
      721 cgccccggac ctgcacctgc cggacggtcg cgcccttggc gaactgctta ccgcctgccc
      781 cttcgcaggt ctggaacgca tagacgaatc acctttgtag ggtggacatg gtgccacctt
      841 gtccaccgct cgcacggcaa tggcggacaa gccttcggca tgcccgccct aaccacgttg
      901 agcctgcttt tcatggcgca taaccgagcg cagcaggttt tattggcggt aacgccagta
      961 acaggccggt aacacctgca attgacttcc agccgcccca tcaggactat aggcgtcccg
     1021 ttgccccgca ccggtgcaat tcgaggccaa tgcaggccaa cttgaggcag taagactgcc
     1081 gactgaacgc ctgagtgagg acagttttca tgcctgatgt aaccctgacc accctgcaca
     1141 gcctcaagca gagcggcgag aagatcgcca tgctgacctg ctatgacgcc acgttcgccc
     1201 agacggcctg ccaggccggt gtcgatgtgt tgctggtggg cgattccctc ggcatggtct
     1261 tgcaaggcca tgacagcacg ctgcccgtca cggttgccga aatggcgtac cacaccgcca
     1321 gcgttaaacg tggcaaccag ggtgcgctga tcctcgccga cctgccgttt atggcctacg
     1381 ccactatcga gcagaccctg aataacagcg cggcgctgat gcaagccggt gcgcatatgg
     1441 tcaagttgga aggtgcggcc tggctggccg agccgattcg ccaactggcc gaacgcggcg
     1501 taccggtgtg cgcacacctg ggcttgaccc cgcaggcagt caacatcctc ggtggttata
     1561 aagtgcaggg ccgtcaggaa tcccaggcgc ggcagatgcg tgccgacgcc atggccctgg
     1621 agcaagccgg tgcagccatg ctcctgctcg aatgcgtacc cagtgagctg gccgccgaaa
     1681 tcagccaagc ggtgaaaatc ccggtgatcg gcattggcgc aggcggcgca accgacggcc
     1741 aggtactggt gctgcacgac atgctcgggc tgtccctcag cggccgcacg ccgaagttcg
     1801 tgaaaaactt tatggctggc cagagcagca tccagcacgc catcagcgcc tacgtcaaag
     1861 cggtcaaaga ccagagcttc ccagcagccg aacacggatt ttctgcatga acacagttaa
     1921 aagcgtgcgc gagttgcgcg ccgctgtcgc ccaggcccgc gccgaaggca aacagatcgc
     1981 cttcgtcccg accatgggca acctgcacgc cggtcacgcc gcactggtgg agaaagccgc
     2041 ccagcgcgcg gactttgtgg tcgccagcat cttcgtcaac ccgctgcagt tcggccccag
     2101 cgaagacctc gacaaatacc cgcgcaccct gattgccgac caggaaaaac tggtcgaagc
     2161 cggctgccac ctgctgttta ctccggatgt cgaggaaatc tacccgcacg gcatggacgg
     2221 ccagacccgc gtcagcgtgc ctggcgtttc cgaaggactg tgcggcgcca gccgccctgg
     2281 gcacttcgaa ggcgtcgcca ccgtggtaag caagctgttc aacatggtgc agccggacct
     2341 ggcaattttc ggccagaagg atttccagca actggcggtg atccgcaccc tggtgcgtga
     2401 cctgaatatg ccgatccaga ttatcggtga gccaaccgta cgcgccgaag acggcctggc
     2461 gctatcctcg cgcaacggtt acctcaacgc cgaacagcgt gccgcggctc ccaccctgta
     2521 tcgcagcctg gaacagatcg ccagcgccat ccaggccggc gagcgcgact accccaagct
     2581 gatcgcccag gcccagcaac tgcagagcgc ggccggcttc cgccccgact acctggaaat
     2641 tcgtgagtcg aacagcctgc gcccggccag cgccgaggat cgccaactgg tgattctggt
     2701 cgcggccttt atcggcagca cccggctgat cgacaacctg gccctcgagc tggagcccag
     2761 cgcctgagtc aggcgggcaa tccgcacgcc tgacggttgc ctgccacccg cccccgccag
     2821 ttacactcaa ggcccggatt gttccgggcc ttttgcattt cgcgacagac cacgacaagg
     2881 aatccacccc caatggccta ttaccagcac ccgcatgacg tcactcagct tcccgcctgg
     2941 caggcgctgc gccagcaccg cgagcagatg aacgacttca gcatgcgcga ggcctttgcc
     3001 agcgacccac aacgctttga ccatttctcc ctgagcagct gcggcctgct gctggattac
     3061 tcgaagaacc tggccaacga aaagactctg gaactgctgg tgcagctggc cgaacaggcc
     3121 ggtctgagcg aatcgatcaa ggcccagttc aatggcgagc gggtcaatgc ctcggaaggc
     3181 cgcgcggccc tgcacacagc cctgcgcagc cccatcggca agcgcctgga gctggatggg
     3241 gtcgacctga tcccgcaggt gcaccgggta ctcaaccaga tgaccgaact ggtcagccgc
     3301 atccacagcg gcctgtggcg cggctacagc gaaaaaccca tcaccgaagt ggtgaatatc
     3361 ggcatcggtg gctccttcct cggcccgcag ctggtgtccg aggcgctgcg cccgttcacc
     3421 cagcgcggcg tgcgctgcca ttacctggcc aatatcgacg gcagcgagtt ccgtgatctg
     3481 actgcccgac tgaacccgga aaccaccctg tttatcgtct cgtccaagtc cttcggtact
     3541 ctcgagaccc tgaaaaacac cctggcggcg cgcgactggt acctggccat gggcggcccg
     3601 gaagccgagc tgcacaagca ctttattgcg gtcaccagca accagcaggc cgccatcgag
     3661 ttcggcatca aggccgagaa tatcttcccg atgtgggact gggtcggcgg ccgctactcc
     3721 ttgtggtcgg ccatcggtct gcccatcgcc ctggccatcg gcatttccaa cttcaaggaa
     3781 ctgctggccg gggcctacgc catggatcag catttcgccc aggccccgct ggcgcagaac
     3841 atgccggtgc tgatggccct gctgggcatc tggtacggca acttctggga tgccaagagc
     3901 caggcgatcc tgccctacga ccattacctg cgcaatttca ccaagcacct gcagcagctg
     3961 gacatggagt ccaacggcaa gagcgtgcgc caggacggca gcccggtgga cttcaatacc
     4021 ggcccggtga tctggggcgg ggtcggctgc aacggccagc acgcctacca ccagctgctg
     4081 caccagggca cccagctgat cccggccgac tttatcgtcc cggtaaacag cttcagcccc
     4141 ctggccgacc accaccagtg gctgttcgcc aactgcctgt cccaggccca ggccctgatg
     4201 cacggcaaga cccgcgccga ggccgaggcc gaactgcgcg ccaagggcct ggacgaggcc
     4261 gaggtacagc gcctggcccc gcacaaggaa atccccggca accgcccgag caatatcctt
     4321 gcgctcaacc gcatcgaccc gttcggcctc ggcgcgctgg tggcgcttta tgaacacaag
     4381 gtctttgtgc agagcgccat ctggggcatc aacgccttcg atcaatgggg cgtcgaactg
     4441 ggcaaggagc tcggccaagg cgtctaccaa cgcctgaccg gacagctcga cacacctgcc
     4501 agcgacgctt cgactcaagg tctgatccag cacttccgcg agcgtcatcg cggctaacag
     4561 ggcttaattg cggcaaaaac gctgacgata ctcatccggg gtaaccccca ggcgctgact
     4621 gaagcagcgc cgcaggttgt acagcgagac aaagccgcac tgctcagcca cgcgtttgag
     4681 tgagcgtgcg ccttgctcca actgacgaca ggcggcctcc agacgcaatt gctgcagata
     4741 atgccccggc gtcatctgca gctcctcgcg gcaacggcgg tgcaaggtcc gcaccgacag
     4801 cgacagccct gccgccatcg cctcgttatc cagtcgctgt tgcagcctgg gttgcagcca
     4861 ctgcttgagg ctctccgcca ggccacccgg cgccccggtc tgcgccagca actcggcact
     4921 gaactggcgt tgcccaccgc tgcgcctaag cggcagcacc aggtccttgg ctacgtccaa
     4981 ggccattgcc gacccgcagt cttcctctac cagcgccaaa cacaggtcaa tcccggcggt
     5041 cactccggcc gagctgtagc agttgccatc cttgataaac agcgcgtcag cgcacacctc
     5101 gatgcgcgca aagctgcgct gcagcagctc gacatagcgc caatgggtaa ccgccatacg
     5161 gccatccagc agcccggcct ggcccagcag aaatgctccg gtacagacag aacaacagcg
     5221 cgccagctgc tcggcggcct gacgcagcca ggcggccagc tgcccgccag ccagcacctg
     5281 catcacgcca aaaccaccgg ccaccagcag cgtcgcccca gccagctgca caatcggcgg
     5341 caaaggctca gtcagcacca ccacacccgc cgaagcacgc acctcgccgc cgtgctgcga
     5401 caccaggcgc aggcgatagc gcggcggcaa accggccgca gccaggcgat cattgaccac
     5461 ggcaaagact tccagcggcc cactggcatc aagcaactgg aaattgtcga agaccagcag
     5521 tagaacgtcc tgcaaatcag cggatgtagg caggggtaat tcgcgcatat ccatcatcct
     5581 tggcacctgt tcaaaggcgc gcgagcgtgc ggcactggca gatattcact caacaatggc
     5641 attactgcca agcgcaccca ggcgagactc aagcctcgac tggagaccac ccatgcctac
     5701 tagccttctg ctgctcagcc tgtgcctcag cctgttgctc gacacaacca ctgcgcgcgc
     5761 cgcaacctcg ccaccgataa gcgacgaagc catcgccgcc tatgtgcccc gccacggacg
     5821 cgcgcgcccg gtgattgccg tgttgggcga gaaccgcatg accgaactgg tcgactttgt
     5881 ggtgccctac gggattctca aacgcgccga tatcgccgag gtgctggccg tcgcccccga
     5941 gcctggcacc atcacgatgc tgccggcgtt gagttttgcc gccgacgcca gcacggccga
     6001 gttcgacgca cgttatccgc aaggcgccga ctacctgatc atcgcggcga ctcaccataa
     6061 cgatgatgtc cgcctgctca cctggattcg cgcgcaagcg ggcaaaggcg cgactatgct
     6121 gggcatctgc gacggcgtat ttagcctggc tcacgccgga ctgctgaacg ggcgacgcgc
     6181 caccgggcat tggtattcgg ccagcgaacg ccgtgaacaa ttcccgcaaa cccaatggca
     6241 agagcaacgt cgttatgtgg tcgatggcgc gatgatcagc agttcgggag tcagcgccgc
     6301 catgccggtt tcactggctc tggtcgaggc cattgccggg cagttacggg cgcagcagct
     6361 tgctgacgaa ctggggctgg ccgactggtc agccgcacac gatagccgcg ccttcaccct
     6421 gcaccgcgct gactacctga ccctggccgg caactggctg gcgttctggg cccatgaaac
     6481 cctggctatc cccgtgcagg tcgggatcga cgaaatcgcc ctggccctga ctgccgatgc
     6541 ctacgcccgc acctaccgct cacaggtgct aagcatcgcc accgcgtcgc tggtgacgcg
     6601 cagcggcctg cgcctgatcc ctgaccgtgc gcacagcaag ccgggcatgc gcagcctcag
     6661 catcgccaag gggcctgcga gccatgcctt gcagcaggcc ctggcggata tagccaaccg
     6721 ccatggccct gcgaccgcgc gactggtcgc cttgcaactg gaatacccca gcccctacct
     6781 tggaccggag tcgccgccct aagcggccac accacttgaa ccaagcaccc gcttgggtgc
     6841 aaccttggga tatcgcggaa cacaacaaca aggacccgcc atgttcgaaa ttacccgcca
     6901 tcccgtgccc gacgccgtgc gccagcgcgc ccacctggac aacgacgact acctgcgcct
     6961 ctaccagcag tcgattgagc agccggaaat cttctgggcc gaacaggcaa aggccttcct
     7021 cgactggagc aaaccctgga ccagcgtgca gcagggcgac ctgctcagcg gtcagggctc
     7081 gtggttcaag ggtggccagc tgaacgtcag ctacaactgc atcgaccgcc accttgaaaa
     7141 gcgcggcgag cagatcgcgc tgatctggga aggcgacaac cccagcgagt cggcacatat
     7201 cacctacaaa aagctgcacc acaacgtcag ccgcctggcc aacgtgctga aaagccgtgg
     7261 ggtgaaaaag ggcgaccggg tgtgcatcta tatgccgatg atccccgaag ccacctacgc
     7321 catgctcgcc tgcacgcgca tcggtgcggt gcactcggtg gtattcggcg gtttctcccc
     7381 ggacgcgctg cgtgaccgca tcctggactc cgattgccac acggtgatca ccgccgatga
     7441 aggcgtgcgc ggcggcaaat acgtggcgct caaggccaat gtcgacaaag cgctgcaaag
     7501 ctgcccggcg gtgtccacag tggtggtggt cgaacgcacc cagggcgaga ttgattgggt
     7561 cgaaggccgc gatatctggt accacgaagc cctgcgcgaa gtttcgggcg actgcccacc
     7621 ggagccgatg gacgccgaag acccgctatt tatcctctac acctccggct ccaccggcaa
     7681 acccaagggc gtgctgcaca gcaccggcgg ctatctgctg atggcggcca tgacccacaa
     7741 gtacgtgttc gactaccacg aaggcgatat ctactggtgc accgccgacg tcggctgggt
     7801 caccgggcac agctatatcg tttacggccc gctggccaac ggcgcgacca gtctgatctt
     7861 cgagggcgtg ccgaactacc cgagcgcctc gcgcttctgg caggtgatcg acaaacacca
     7921 ggtgaatatc ttctacaccg cgcccaccgc cctgcgcgcg ctgatgcgtg aaggcagcgg
     7981 cccggtcgaa tgcaccgagc gcaccagcct gcgcctgctc ggcagcgtcg gcgagccgat
     8041 caacccggaa gcctgggagt ggtattacaa cgtggttggt gagcgccgct gcccgatcgt
     8101 cgacacctgg tggcagaccg aaaccggcgc catcctgatc accccactac ccggcgccac
     8161 cgacctcaaa cccggctcgg cgacccggcc gttcttcggc gtacagccgg tgctgctgga
     8221 tgaacagggc aaggagatcg acggccctgg atcaggcgtg ctggcgatca aggacagctg
     8281 gccaagccag atccgcagcg tctacggcga ccatcagcgg atgatcgaca cctatctgaa
     8341 gccctatccc ggttactact ttagcggcga cggcgcacgc cgcgacgagg acggctacta
     8401 ctggataacg ggacgtgtgg acgacgtgat caacgtctcc ggccaccgca tcggcaccgc
     8461 cgaagtggaa agcgccctgg tgctgcacga cgccgtggcc gaagccgcag tggtcggcta
     8521 cccgcacgac ctcaaaggcc aaggcatcta cgccttcgtt acgccgatga atggcgtgga
     8581 aatcaacgac gcgctgaaga aggagctgct ggccctggtc ggcaaggaaa tcggcagctt
     8641 cgccaaaccc gagctgatcc agtgggcgcc cggcctgccg aaaacccgtt cgggcaagat
     8701 catgcggcgt atcctgcgca agatcgcctg caacgaactg gaaaacatgg gcgacacctc
     8761 gaccctggcc gaccccacgg tggtggacgg gctgatcgag caacgcctga accgctaaca
     8821 ccagacgtag agcagccatg tgcacagcat ggctgccctg cagagcccct atggaactcc
     8881 ttcgccgccg tatcgaaagc caagtcctta gcctgagcgg tatcgccctt ggccagatcg
     8941 actttgaaaa gccccagggc gaccccggcc tgttcggccc ggactcggta agctggcagg
     9001 tacatggcga cttcaccagc atgctgatcg gcggcatcag cgccctgctg ctgcaagccc
     9061 tgcatccact ggcattggcc ggggtgtggg accactcgaa ctttcgtgaa gacctgctcg
     9121 gccggctgcg ccgcaccggg cagtttattt ccggcaccac ctacggctcg cgtgccgatg
     9181 ccgactggct gatcgacaag gtcaagacca ttcacctcaa ggtcagcggc accgcgccgg
     9241 atggccgcgc ctatgccgcc agtgacccgg cattgttgac ctgggtgcat gtggccgaag
     9301 tgcacagctt tctccaggcc cacctgcgtt accgcaaccc acaactgagc gaagccgatc
     9361 aggaccgcta ctacgcggaa atcgccttga tcgctgaacg cctgggcgcc acccaggtac
     9421 cgcgctcgcg cgccgaaatc gccgcctacc tggcagctgt gcgccccgaa ttgctttgcg
     9481 atgaacgctc gctagagatt ctgcgcattc tgctcggcgc accgcccccc agcgccctcg
     9541 ccgccccgcc ggccaagctg ttgatgcagg ccggtatcga cctgctgccg gcttgggcgc
     9601 agcagatgct cggccagcag atcagcccgt cgcgtagccg gatgatccac gccggcgtac
     9661 acagcctggc ccccgtgctg cgctgggcgg tacgtggcgg cgccatccac cgcgcgcgca
     9721 aacgtttgca gttgccgccg cgctgaccgg ccggttcgcg cgctggccgt aaacaaagcc
     9781 gctgtgccac catagcgacc tctcgcggcc tgccgcgcct gttacttgtg aggatcgcgt
     9841 tatgcaagac gtcgtcatcg ttgccgccac ccgcaccgcc atcggcagct ttcagggtgc
     9901 gctggccaat atcccggcac cggaactggg cgctgcggta atccgccgcc tgctggaaca
     9961 aaccggcctg gacggcgcgc aggtcgacga agtgatcctc ggccaggtgc tgaccgccgg
    10021 cagcgggcag aacccggcac gccaggcggt gatcctcgcc ggcctgccgc acgccgtacc
    10081 agccctgacc ctgaacaagg tctgcggatc gggcctgaaa gccctgcatc tgggcgccca
    10141 ggcgatccgc tgcggcgacg ccgaagtgat catcgccggc ggcatggaaa acatgagcct
    10201 ggccccctac gtgatgcccg gcgcccgcac cggcctgcgt atgggtcacg ccaagctggt
    10261 cgacaccatg atcaccgacg gcctgtggga tgccttcaac gactaccaca tgggcatcac
    10321 cgccgagaac ctggtggaca aatacaacat ttctcgtgaa gaacaggacg ctttcgcagc
    10381 cgcctcacag cagaaagctg tggccgccat cgaagccggg cgctttgctg atgaaatcac
    10441 cccgatcatg attccccagc gcaagggcga gccgctggcc ttcgccactg acgagcagcc
    10501 gcgcgccggc accactgctg aatccctggg caaactcaag cccgcattca agaaagatgg
    10561 cagcgtcacc gccggcaacg cctccagcct gaatgacggc gcctccgccg tgctgctgat
    10621 gagcgcggcc aaagcccagg ccctcggcct gccagtactg gcgaagatcg ccagctacgc
    10681 caacgctggt gtcgacccgg cgatcatggg catcggcccg gtcagcgcca cccagcgttg
    10741 cctggctaag gctggctgga gcatcgctga tctggacctg atcgaggcca acgaagcctt
    10801 cgccgcacaa gccctggcgg tcggcaaaga gctgggctgg gacaccagca aggtcaacgt
    10861 caacggcggc gcaatcgccc tcggccaccc cattggcggc tccggctgcc gcgtgctggt
    10921 gaccctgctg cacgaaatga tcaagcgcga cgccaagaaa ggcctcgcca ccctgtgcat
    10981 cggcggcggt cagggcgtgg cgctggcgat cgaacgggcc tgataacagc gttcagctat
    11041 cgtgacgaaa acgccggcga cccgaactaa cgggagccgg cgtttttctt gataaactgc
    11101 gcgccctcct ttttcgagtc gccgcgcatg ccctctctcg atcaggcgct gcgcgccgca
    11161 ctgcataacc gtcaagacct gctggccaag ctgcatcagc aaggcactga ctgctaccgc
    11221 ctgttccatg gcagccagga aggtgccagc ggccttacca tcgaccgtta tggtccgcaa
    11281 ttgctggtgc agagctttca ccagagtctg agtcgtgacg aactgctgca actggccgag
    11341 cagtgccaga ccagcctcgg cacacaactg ctactggtct acaacgaccg ctcccagggc
    11401 aactcgcgga tcgaccgcac tgatacggtc taccaagcca atccagccgc cctggaagac
    11461 ttggtcggcc atgaatgggg gctcaattac cgcgtgcgtg gccgccatgc tgggcaagat
    11521 ccgctgctgt ttctcgacct gcgtaacgcc cgcggctggg tcaaagcgca cagcgccggt
    11581 aaatcggtgc ttaacctgtt tgcctacacc tgtggcgtcg gcttgagtgc ggctgcaggc
    11641 ggagccaacg aggtgcttaa cctggatttt gccgagggca atctggcggt cggtcgcgag
    11701 aatggtcagc tcaatccgca gctgctaccc atgcagtttg tccagtcgga ctacttcccg
    11761 gcgattcgcc aactggccgg tctgccgatc aatgcgcggc gcgggcagaa gctgccgcac
    11821 tacccgcgct ttgagcagcg tcagttcgat ttggtgctgc tcgacccgcc cgcctgggcc
    11881 aagagcgctt tcggcacggt cgatttgctg cgcgattacc agagcctgct caagccggcg
    11941 atcctcgcca ccgccgatga tggcgtgctg atctgctgca acaacctggc aaaagtcgcc
    12001 atggccgatt ggcgcgagca ggtgctgcgc tgcgcggaaa agctcggccg tccggtacgc
    12061 gattgccagg tgctgccgcc agccagcgac ttcccctcac aggatggcca gccaccgctg
    12121 aaaaccctga tcctgcagtt ctgatccacg cgcaagttcg cagaaaatcc ccgcgcagag
    12181 cctctgcgga actgcacacg cgtgccatac tccaaggcac ttctcgtcgg gatagatgac
    12241 gtttttatgc ttaaaggaat aaagcgcgct gccagcgcca ttgccatcgc cgttgccctc
    12301 tacagcctgc tgggctttct gattctgccc ggtatcggtt tgcgcatcgc caaccagcag
    12361 ctggcgcaat acgccgaagt acccgccacg ctgcaacgcc tgcaactcaa cccgttcagc
    12421 ctggagctca gcctctgggg cctgcagatt ggcgaagccg aacagcaaca gatcgccttc
    12481 gaacgcctgt atgccaacct gcaactcgat agcctgtgga gcggcgtatt gcacctggct
    12541 gatatcgagt tggacaagcc gcacagcgaa gtgctgttcg gcaaggacgg tcggcttaac
    12601 ctgacccaat tgttcaaagt gccacccagc ccgcaaccag cagtcgaaga accggccggc
    12661 gagccgttcc cgctgcgtat cgcgcggatc aagctgagtg gcggcaacgt gcactttcag
    12721 gacctgcgcc ccagcgagcc gatcgagttt atctacgacg acctcaactt cgagctgttc
    12781 aacctcagca ccctgcccga cgacaacgcc gacatgaacc tcgttgccac cggcccccaa
    12841 ggcgggcgca tcgactggac cgggcgcatc ggcctggtac cgatcagctc caacggcagc
    12901 ttgaaagtca ctgacggcaa gctgaaagcc atctggccct acgtacgcga tgccgtaccg
    12961 ctggtgctgg aggacggcac cgtcaacctc agcagcgact acagcctgaa ccttgccaaa
    13021 ggcaccgagc tactgctgag caacacccag ctcagcgtca gcagctttgc catcaagaac
    13081 ccggcggaca aaccactgct gcgcctggaa agcctggata tcagcgaaac cagcgtagac
    13141 ctggccaagc aacaggtcac cgtcggcaag attcgtagcc agaagctgga aacctgggcc
    13201 gcacgcgaag ccgatggtca actggactgg cagaaactgc tggccagcca acccgctaaa
    13261 ccagctccag ctccagctcc agctccagct ccaaatgagg gtggcgccgg ctctgcggca
    13321 acggaagccg agccggtaaa cgacccaacc gagccgacca ccgcagagca atccgctgat
    13381 ttggaaacgg ccacggccgg ccagccggac gagcctagca aaccttggca agtcatcctt
    13441 cgtgacgtgc aattgcgcga ctatcaggtg catctgaccg accgagccgg cggctcggaa
    13501 gtcaaagtcg acctttcccc gctcaatctc gacctgagcg acttcgacag cctgggcacc
    13561 tcgcccttcg ctctcaagct cgataccggg gtaaacaaga ctgggcagat caaggctgac
    13621 ggccaggtgc aactgacccc gaccacagcc aagctgcagg ttgccactcg tgatatcgat
    13681 ctgcgcctgg cgcagaccta cctgagcccc ttcgtccgtc tggagctgcg cagcggcaag
    13741 ctcggcagcg acttggcggt tgatctgcag agcgtcgagc cgctggcctt cagcattggc
    13801 gggcaagccg agatcagcca gctgcatacc ctcgacacgc tcaaggaccg cgacttcctc
    13861 aaatggcagc aagtgctggt ggaagggatc gactatcaac acggcaaggg cctggtgatc
    13921 ggccaggtca acctgcagca gccatatgtg cgctttatca tcaacgaaga cctgaccacc
    13981 aatatcaacg acctgatgat tccacagccg gcagaggcca acgccaaacc cgctgcggca
    14041 agtaaaccgc tgccgatgcg catcggcggt atcagcatca aggacggttc ggcgaacttt
    14101 gccgatttca gcctgacgcc caacttcgcc acggctattc agcaactcaa cggccagatt
    14161 ggcacactcg acaaccagag cccgaaaacc gccagcgtcg atattcaggg caaggtcgac
    14221 aagtacgcgc ccgtcagcat caagggcaag ctgaccccgt tcgacccgat gaacagcctg
    14281 gatatcgcca ccagcttcaa gaatgtcgag ctgaccaccc tcacgcccta ctccggcaag
    14341 ttcgccggct tccgcatccg caagggccgc ctcaacctcg acctgcatta ccagatcgag
    14401 aaaggccagc tcaacgccga gaacaaactg ctactggaag acctgcagct gggcgagaaa
    14461 gtcgacagca aagacgccat ggacctgcca attcgcctgg ccattgccct gctcaaagac
    14521 accgacggca atatcagcat ccagctgccc gtgcagggcg acctcaacag cccgcagttc
    14581 agcgttatgc ccatcgtctg gcagaccctg cgcaacctgg tactacgcgc cgcgcaggca
    14641 ccgttcaaat ttatcggcgg cctggtcagc ggcggcagtg acgttgacct cagcaccgtg
    14701 cgcttcaatg ccggtgccag cgagctcgac ggc
//
LOCUS       DASRUM010000293        13584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_663199, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000293 DASRUM010000000
VERSION     DASRUM010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 13584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13584
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663199"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="VFF22_19450"
     CDS             <1..147
                     /locus_tag="VFF22_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HZX19031.1"
                     /translation="GDYQLCFNQRCHSMSEWLGPPQATQPALELWSCEQSSPAASAAG
                     QDDG"
     gene            481..993
                     /locus_tag="VFF22_19455"
     CDS             481..993
                     /locus_tag="VFF22_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZX19032.1"
                     /translation="MSEKGDIITYYLDMQHPEQIRAKALPGDLSVVECQLKQFQLNRF
                     LYQLVGEPWQWTDKLSWTDAQWRELIEQSNHRTWVAYHQGAIAGYYELLRDADGAVEI
                     LYFGLAEAFFGQGFGGPLLTQALQSAWAWPGTTKVWVHTCSLDHPSALANYQARGLRI
                     YREEVEADAL"
     gene            1040..1555
                     /locus_tag="VFF22_19460"
     CDS             1040..1555
                     /locus_tag="VFF22_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HZX19033.1"
                     /translation="MRRNLSKPLLLSLCLLPALASALDIQPGLWEISSNNMQVGGQAV
                     PGMEQMLEQMKNLPPEQRQMMEQMMAQQGVQLGDKGVRICMSEAQIKAQQIPMQDPES
                     GCSNEVTERSDELWKFRFSCPNGQGEGETRFVSDKEFTSTVNGTFDGQSSSMQNHARW
                     VAADCGTLQPQ"
     gene            complement(1566..2309)
                     /locus_tag="VFF22_19465"
     CDS             complement(1566..2309)
                     /locus_tag="VFF22_19465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX19034.1"
                     /translation="MRALLTLLACLPFTLASLTQAETFKVGVELQPYLPYSNVQDGQY
                     LGYGRDLLDAFAAHQGHEFIYQPLPVRRLLSDFLNGRVDFKYPDNPRWNADLKQGHKV
                     HFSQAAAPAIDGVLVKPQFLGKGKARLLRLGTQRGFTPWPYLDEIKAGKILLIQANQI
                     DSLLAMAMSDRIDGVYLNPQVVRHQLYNSAKTLVFDPSLPYQDDHYFLSSINHPEVIE
                     QFDAFLTSQAELVQTLKERHGISDPLNGQ"
     gene            2586..4253
                     /locus_tag="VFF22_19470"
     CDS             2586..4253
                     /locus_tag="VFF22_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HZX19035.1"
                     /translation="MEKDPSSDTTPQPEAAILDGIPAPSGEANLIDTDYVIGQDNIKG
                     QFSFALDIHGKVFSISALTIVLFVVLTLALQNHVEPLFSAMRGWLTQHLAWFFLGSAN
                     IFVLLCLGLIVSPLGKVRLGGMDATADYSYAGWFSMLFAAGMGIGLMFYGVAEPMGHF
                     SAAMGGVSVGEDGLRTDWAPLAGAAGDAEAAARLGMAATIFHWGLHPWAIYAIVALAL
                     ALFSFNKGLPLSIRSIFYPILGERVWGWPGHIIDILAVFATLFGLATSLGLGAEQAAA
                     GLDKLFGIAATDASKVLLIIGITAIALLSVLAGLDKGVKRLSELNMGLAILLLLFVII
                     AGPTLAILTGFFDNLGAYLQHLPALSNPIAREDANFSQGWTAFYWAWWISWSPFVGMF
                     IARVSRGRTVREFLIAVLLVPSLVSVLWMTAFGGTGISQLVGDGFTGVQDAALELKLF
                     AMLAELPLAELSSFIGIVLVIVFFVTSSDSGSLVIDTITAGGKVNAPVPQRVFWVIIE
                     GVIAIALLLGGGLVALQAMAVSTGLPFTIVLLVGCVSIVKGLMSEPR"
     gene            complement(4800..5060)
                     /locus_tag="VFF22_19475"
     CDS             complement(4800..5060)
                     /locus_tag="VFF22_19475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX19036.1"
                     /translation="MPRNRREYGLSPEDRDWVINKKFGSLYREKAEEILQKSTDKSDK
                     ILGAILFLSRPGNLDDLASNVDLANESLQKLLDAATVKDERT"
     gene            complement(5136..5693)
                     /locus_tag="VFF22_19480"
     CDS             complement(5136..5693)
                     /locus_tag="VFF22_19480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX19037.1"
                     /translation="MHRGPAKGIKISLPIKLGLAFLAGFLAWAVGPLGLLDKDVVSEI
                     PAWILPGIIFGALLLVPHSRWVSPGRAVAALGIGVFAWVAAFAVGGFGWWVGGWLSFV
                     AAGAISGTILGLLVPRLIQSRQYFRYFMLLSFAGFLGSIPFAMCFRTMDWKDALFAPQ
                     WVIPSYIFWNMVIAIALHLSATRDD"
     gene            complement(5781..6029)
                     /locus_tag="VFF22_19485"
     CDS             complement(5781..6029)
                     /locus_tag="VFF22_19485"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HZX19038.1"
                     /translation="MTINSSKFEAVSLTEASFKDVNLKLANFTDVNLAGSSFSDVSLA
                     GAKFHNVDLSNVAITDANLEGMVINGILVTELLRVYGA"
     gene            complement(6126..6542)
                     /locus_tag="VFF22_19490"
     CDS             complement(6126..6542)
                     /locus_tag="VFF22_19490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX19039.1"
                     /translation="MENLNFIDAIAFLSSIASLILAVVAILAAKSSEREVRENFEKTQ
                     RVMMEYQERTKEVLAEIDKKSEVIERTVSESQRQLMNTMTNIINETVIPKKVDMGEQL
                     GMQFMQQMFTNPEKANEMMGGLAQMMKIAEMQKPKK"
     gene            complement(6618..7457)
                     /locus_tag="VFF22_19495"
     CDS             complement(6618..7457)
                     /locus_tag="VFF22_19495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX19040.1"
                     /translation="MLDETLRLIKEIIWDSVKAISTLTAVGIISYKVYLTPIALTVDF
                     PTLLSLLLALFSVALAALFYFKATDTSNTFYDNTYNFTKDIAQLLVKMESGFGEKLRN
                     LDEGYSSMREYLQTYPSRGTGIDDTKRKIEGEKQEIEKVIEERNKIVNDLIEKSQLEQ
                     EEKEQILLQLKSKESELEASQKELEKMNKRLFLERIEKKNFKNELRPGLESYTYSHVI
                     EKIGLDRVLHLSSIGLQKRVDALLNELPRQYIEDLERNGYFDNGINVKGVNFIRRLAE
                     KHA"
     gene            complement(8090..8803)
                     /locus_tag="VFF22_19500"
     CDS             complement(8090..8803)
                     /locus_tag="VFF22_19500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZX19041.1"
                     /translation="MTTKLATPLDWAARFAELAASATDSRLQAFYQAGCVAADTPLEQ
                     VPLLALDVETTGLDANQHAIVSLGLLPFNLQRIRCGAAHYWVVKPACELSGESVTFHH
                     ITHSDIRHAPRLGQILDELLTAMAGKVMVVHYRTIERSFLDQALRQQLGEGLQFPVID
                     TMQLEARLYPKRQPGWLGRLLGRQPVSIRLADSRLRYNLPMYQAHHALTDALATAELL
                     QAQVATRYSGDTPVGELWS"
     gene            complement(8800..10674)
                     /locus_tag="VFF22_19505"
     CDS             complement(8800..10674)
                     /locus_tag="VFF22_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HZX19042.1"
                     /translation="MQIELLEIRDHLQRFPPFDALPEDNLDAIARQVEVTYFKAGSDI
                     LQYGAAIHELHYVRSGAVEIYRRNGELYNRLSEGDIFGQAGLLRSNKVRFPARAIEDS
                     LIYFIPATLFAQLCEEHDGFAEFVEAEGQSRLKSAVESQGQASELIQLKVRKLISRPP
                     VCVSLDTPVQQAAQLMTEQSVSSLVIVDGDAPGAAMVGILTDRDLRTRVLAAGLASST
                     AVSQVMTPEPFTVQADDSVFEAMLCMLRHNIHHLPVVQRRRPIGLINLSDIIRYESQS
                     SLYLVNSIFNQPNVAGLQSLLADLRGTFVRMVNEQATAHMVGSAMAGIGRAFTQRLLE
                     LAEQKLGPPPVPYCFMVAGSMARDEQLLVTDQDNALVLDDRFDPAQHDAYFAALAQFV
                     SDGLAACGYSYCKGGIMASNAQWRQPLSVWRGYFNQWIDKPKPQTLLHSCIFFDLDGV
                     YGEFELVENLKDLLAQKASSNPAFLAALARNALNRTPPLGFFRTFVMETDGQQKNIIN
                     LKGRGTAPLTDLIRVHALACGSKAQNSLERLDAIGKTKLLQPEAISQLRYALEFLSLV
                     RARHQAMAAADGAQPNNYIEPEKVSASDRHNLKEAFQVISNAQKFLRFRYPAQPGKSL
                     "
     gene            10960..11526
                     /locus_tag="VFF22_19510"
     CDS             10960..11526
                     /locus_tag="VFF22_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZX19043.1"
                     /translation="MQGAGFWVVLALLLALAGLTIYYYNRLVFNRARVAEAWSGIDVQ
                     LKRRASLIPALVECVRGYMNYEQDTLQALVQERLRAVQLADSPLGERAPVEAHLGTQL
                     RQLFALVEDYPALKADGQFLDLQRNITDVENHIQMARRYYNGAVRELNVLVESVPSNL
                     VARPFAFTQAEYFSLDDPRDAQSPKMEF"
     gene            11528..13456
                     /locus_tag="VFF22_19515"
     CDS             11528..13456
                     /locus_tag="VFF22_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HZX19044.1"
                     /translation="MLRVLRFALLSLCLLPLWAAAAEVIEDFTVTLQVQADGNLLVTE
                     RITVQAEGEQIRRGIYRDLPVSYSMALGLQKSGPISLLGVTRDGQAERVTTERNGAWV
                     RYYIGSADHLLQPGRYQYELRYRVERQLLHHSTTDELYWNVTGNQWVFPILQASVEVQ
                     LPPGARIGQLAAYTGAHGEQGKAFEVVEQRDDYLRLVTTQALSAYHGLTVAVDWPAGL
                     VARPGIAQRLGYLLLDNLGLCLGALLLVGLVIFYLRAWDRVGRDPQKGLIISLFEAPQ
                     GMSAVQAGYLWHRGLKGPYQAARAFSVWLTDMAIRKHLHLEDKPRGGGFTLARGTGER
                     DDFSEGDRDLRKRLFPANKEGIALEIGSDYEPRLADAVAGLTTQLQTQGKAWFSNNRG
                     IWAWGLVWALVACLVMVFSGAQGEDDIATGLAGVMFTLGFGVPSLFVLYMAWNQPSLG
                     KQIGLGLAGLMFVWPVPIGLWMLSDAASVPALLLLCVYVLVVVLFYYLLPAPSIEGRR
                     LLDQLEGYRDYLQLAESEALALAGNAPAMSIALYEKHLPYAMALGVEDKWSARFSTAL
                     ASGLIDPAQRDYQPDWYHSRSSFSTPLAMSAALAAGLSSATALASSPPSSSSSGGGGS
                     SGGGSSGGGGGGGGGGGW"
BASE COUNT         2576 a         3789 c         4187 g         3032 t
ORIGIN      
        1 ggggattatc aactgtgttt caaccagcgt tgccactcga tgagtgaatg gctcggcccg
       61 ccacaagcga cccagccggc gttggaactc tggagctgtg agcaaagctc cccggccgcc
      121 tcggcggcag gccaggacga cggctaaccc cgtgtccgct cccaaggcca caagccctgg
      181 ttgttgtaga ggagaacccc catttggctt ggccggtggg ggttttgttc gttatggcct
      241 ggcacatctg caaacaggcg ttcactttac gaatgaaagt ttagcttcgg ccatatgtaa
      301 gtttcatatc gtccatgtgt aaaaaagtgt tcggccatat gtaagttttt agaaggctct
      361 ggttgcccga ttcattctgt ttaaagagat atcttcaaca ggattgccga acccatagcg
      421 ggtcattagc ctgcgttttt tcatgggcta gatggcctta ccagagcggc ggaagacggc
      481 atgagcgaga aaggcgacat catcacctat tacctggata tgcagcaccc cgagcagatc
      541 cgcgccaagg ccctgccggg cgacctgagc gtggtggaat gccagctcaa gcagtttcag
      601 ctcaaccgct ttctctacca gctggtcggc gagccctggc agtggaccga caagttgagc
      661 tggacagacg cgcagtggcg cgagctgatc gaacagtcca atcaccgcac ctgggtggcc
      721 tatcaccagg gcgctatcgc cggttattac gagctgctgc gtgatgccga cggcgccgtg
      781 gaaattctct atttcggcct ggctgaggcc tttttcggcc agggttttgg cggcccgctg
      841 ctgactcagg ccctgcaatc cgcctgggcc tggccgggca ccacaaaggt ctgggtgcat
      901 acctgcagcc tcgatcaccc cagcgcgctg gccaactacc aagcgcgcgg cctgcgtatc
      961 tatcgtgaag aggtcgaggc ggacgctttg taagagggtc tacgcttgca aggtcgccca
     1021 cttatttcag gagccattca tgcgccgcaa ccttagcaaa cccctgttac tcagcctctg
     1081 cctgctgccc gccctggcca gcgcgttgga tatccagccc ggtctctggg aaatcagctc
     1141 gaacaatatg caggtcggcg gccaggcagt gccggggatg gagcaaatgc tcgagcagat
     1201 gaaaaacctg ccgcccgagc aacggcaaat gatggagcag atgatggcgc agcagggcgt
     1261 acagctgggc gacaaaggcg tgcgcatctg catgagcgaa gcgcagatca aggcccagca
     1321 gatccccatg caagaccccg agtccggctg cagtaatgaa gtcaccgaac gcagcgacga
     1381 gttgtggaag ttccgcttca gctgccccaa cggccagggc gaaggcgaga cgcgctttgt
     1441 cagcgacaag gaattcacct caacggtcaa cggcaccttc gatggccaga gcagcagcat
     1501 gcaaaaccat gcgcgctggg tggcggcgga ttgcgggacg ttgcagccgc aataagcagc
     1561 cgaggtcact ggccgttaag cgggtcgctg atgccgtggc gttcttttag cgtttgcacc
     1621 agctcggcct ggctggtgag gaaggcgtcg aactgctcga tcacctcagg gtggttgatg
     1681 ctcgagagga agtagtggtc atcctgatag ggcaggctcg ggtcaaacac cagggtcttc
     1741 gcgctgttgt agagctggtg gcgcaccact tgcgggttga ggtaaacgcc atcaatccgg
     1801 tcgctcatgg ccatggccaa cagcgagtca atctggttgg cctggatcag caggatcttg
     1861 ccggccttga tctcatccaa gtaaggccag ggggtaaagc cgcgttgcgt gccgaggcgc
     1921 agtaggcgtg ctttaccctt gccgaggaac tgcggcttga ccagcacacc gtcgatggcc
     1981 ggcgccgccg cctggctgaa gtgcaccttg tggccttgtt tcaggtcggc gttccagcgc
     2041 gggttgtccg ggtatttgaa atccacccga ccgttgagaa agtcgctgag caggcgccgc
     2101 accggcagcg gctgatagat aaattcatgg ccctggtgcg ccgcaaaggc gtcgagcagg
     2161 tcgcggccat aacccaggta ttggccatcc tgcacgttgg agtaaggcaa gtagggctgc
     2221 agctcgaccc cgaccttgaa ggtctcggcc tgtgtcagcg aggccagggt aaaaggcagg
     2281 caggccagca gcgtcagcaa tgctctcatc agcgacatct cgaacagcga ccccgagcag
     2341 gccataggcc aaccatagct aatagcctcg ccctgcggga ggcaaagtgc cgagaaaact
     2401 gaccaggcag agcaaataac caccgcgcag cgggctgcaa tggccgccaa tactggcttt
     2461 gcgcctgcac gcatgggcta tggctgttac cctgtacccc gcagctgccg gttgggcggc
     2521 tgtacgagta gggcaatccg tcctcgtgcg cgtaatgcgt gccacacgcc aaaggagcat
     2581 tccatatgga aaaagaccct tcgtctgaca cgaccccgca accagaagcg gcaattctgg
     2641 acggcatccc cgccccctcg ggcgaggcta atctgatcga caccgactac gtcataggtc
     2701 aggacaacat caaagggcag ttctccttcg ccctcgatat ccacggcaaa gtcttcagca
     2761 tttctgcact gaccatcgtg ctgtttgtgg tgctgaccct ggccctgcaa aaccatgtag
     2821 agccgttgtt cagcgccatg cgcggctggc tcacccagca cctggcctgg ttcttccttg
     2881 gttcggccaa catcttcgta ctgctctgcc tcggcctgat cgtctcgccg ctgggcaagg
     2941 tgcgcctcgg tggtatggac gctaccgcgg actacagcta cgccggctgg ttctccatgc
     3001 tgtttgccgc cggtatgggc atcggtctga tgttctacgg cgtggccgag ccgatggggc
     3061 acttctcggc ggccatgggc ggagtcagcg ttggtgagga cggtttacgc accgactggg
     3121 cgcccctggc tggcgctgcc ggtgacgccg aagccgctgc gcgtttgggc atggctgcga
     3181 ccatcttcca ctggggcctg cacccctggg cgatctacgc catcgtcgcc ctggcactgg
     3241 cgctgttctc cttcaacaag ggcctgccgc tgtcgatccg ctcgatcttc tatccgattc
     3301 tcggtgagcg cgtgtggggc tggccggggc atatcatcga catcctcgcg gtgttcgcca
     3361 ccctgttcgg cctggctacc tccctggggt tgggtgcgga acaggccgca gccggcctgg
     3421 ataaactgtt cggcatcgcc gccacggacg cgagcaaggt gctgctgatt atcggcatca
     3481 ccgccatcgc cctgctgtcg gtgctggcgg gtttggacaa gggcgtcaaa cgcctgtccg
     3541 agctgaacat gggcctggcc attctgttgc tgctgttcgt catcatcgcc gggccaaccc
     3601 tggccatcct caccggcttc ttcgacaacc tcggcgctta cctgcagcac ctgccggcgc
     3661 tgtccaaccc gattgcccgt gaagacgcca acttcagcca aggctggacg gccttctact
     3721 gggcttggtg gatcagctgg tcgccgttcg tgggcatgtt tatcgcccgc gtcagccgtg
     3781 gccgcaccgt gcgcgagttt ctgatcgccg tgctgctggt gccgtcgctg gtgtcggtgc
     3841 tgtggatgac ggcttttggg ggcactggca tcagtcaact ggtcggcgat ggctttaccg
     3901 gtgtgcagga cgccgcgctg gagctgaagc tgtttgccat gctggcggag ctgccgttgg
     3961 cggagctcag ttcgtttatc ggcatcgtgc tggtgatcgt gttcttcgtc acctcgtcgg
     4021 actccggctc gctggtgatc gataccatca ccgccggcgg caaggtcaac gcgcccgtgc
     4081 cgcagcgagt gttctgggta attatcgagg gtgtgattgc cattgccctg ttgctcggcg
     4141 gcgggctggt ggccttgcag gccatggcgg tgtcgacggg gttgccgttt accatcgtgc
     4201 tgttggtggg ctgtgtgtcg atcgtcaaag gtttgatgag cgaaccgcgc tgatcattgc
     4261 ttttgagtag tacaagagcg cgcctttggg cgcgcttttt tatgcccgga gtttgtgtgg
     4321 gctggcaccg gcccctgtag gagcgggctt gcccgcgatg ctttgttttg cacccctctc
     4381 ccgtttctac gggagagtgg ttgggggaga gggcgctttt agtgttcggc atggcaccgc
     4441 gtgcgctgat ggcttgtgtt gcgccccctc gggcgcgtca ctttttcttg cttgcccaag
     4501 aaaaagtaac caaaaagaag ggcaccccga catccgggtc tcgctgcgct cgacttccct
     4561 ccctccggca ctgttccggg ggccggcgta catgggccat ccttggccca ttacgcttct
     4621 cgccgtatcc atgcggctcg tccccctaca cagcgcctgc gctcggcctc ctgacggggg
     4681 gttggcgcgc ggctgaaagt gtgtggtttc tgagctggaa agaactaaaa agcgaacggg
     4741 aaagggacgt tttttgttat tcgcagaaca aaagcaaatc cgccccttaa cggcctcctt
     4801 tacgtgcgct catcttttac agtcgcggca tcaagtagct tttgtaggct ttcattcgct
     4861 aggtctacgt tgctagcgag atcatccaga tttcctggcc tggacaaaaa cagaatagct
     4921 cccagaattt tgtccgattt atccgtcgat ttttgcagga tttcttctgc cttttctcga
     4981 tagagagagc caaatttttt attgattacc caatctctat cttcagggct taaaccgtat
     5041 tcccttctgt ttcttggcat aaccctcaag cctcgaatta cacataatgt tgacgtgaac
     5101 ggcaccgctg gttgggcata cgctcgatgg aatggttagt cgtctctagt ggcactgagg
     5161 tgtagtgcta ttgcgatgac catattccag aaaatgtacg acgggataac ccactgtggc
     5221 gcgaacaatg cgtctttcca atccattgtt cggaagcaca ttgcgaaggg aatgctgcct
     5281 agaaatcctg caaaagaaag cagcatgaag tagcgaaaat actgccgaga ctgaatgagg
     5341 cgtggaacga gaagcccaag tatcgtcccg cttatcgccc ccgctgcgac aaaggaaagc
     5401 caaccgccga cccaccagcc aaagcctccg acagcgaatg cagcgaccca cgcgaacacg
     5461 cctatgccga gcgcagccac tgcgcgaccg ggactgaccc aacgactatg gggaacgaga
     5521 agcaaggcac cgaagataat ccccggcaag atccaggcag gaatttccga tacaacatcc
     5581 ttatcaagaa ggcctaacgg accaactgcc caggcaagaa atccagccaa aaaggccagt
     5641 ccaagcttaa taggcaatga gatttttatg cctttagcag gccctctatg catcaacaca
     5701 ctccagtgat gcctaacgtt tagttggcct ccgctgcgcg aattacagaa gggccaggga
     5761 gataaagcct atacgacttg ttatgcgcca tatacgcgaa gcaattcagt cacaagaata
     5821 ccgttgatca ccatgccttc tagatttgcg tcagtgattg caacgttgct taagtcgaca
     5881 ttgtggaatt tggccccggc taacgaaacg tcggagaacg acgagccggc aaggttaacg
     5941 tcagtgaagt tagcaagttt caggttgaca tctttaaagc tcgcctcggt taatgaaacg
     6001 gcttcaaatt tcgatgagtt tatagtcact gttagctctc catgctgtgt gcttgacgat
     6061 taagctaagg ggccggcttc gccggtctca gcgagcgatt tgagcgtatt gttatacgca
     6121 aacactcatt tcttgggctt ttgcatttcg gctattttca tcatttgagc taagccaccc
     6181 atcatctcgt ttgctttttc tgggtttgtg aacatttgct gcataaactg catacctaac
     6241 tgctcgccca tatcgacttt ttttggtatc acagtttcat taataatatt tgtcattgtg
     6301 ttcatgagct gtcgttgtga ctctgaaact gttctttcaa ttacttctga ttttttatca
     6361 atttctgcaa gaacttcttt cgtgcgttct tggtactcca tcatgactct ttgtgttttt
     6421 tcgaaattct cgcgaacttc ccgctctgaa ctttttgctg ccaaaattgc aacgacggca
     6481 aggatcaaag atgcaatgct tgataagaac gctattgcat caatgaagtt aaggttttcc
     6541 atggcatttc ctttgtgtat aacaattaag ctaaggggcc gcggaaggcg gccccagcga
     6601 acgaagtgag ctacttgtta tgcatgcttt tctgctaggc gacgtataaa gttaacgcct
     6661 tttacattaa taccattgtc aaaatatccg tttcgctcta agtcctcaat atattggcga
     6721 ggaagttcat tcagcagggc atcaaccctc ttctgcaagc cgattgagct caggtggagg
     6781 acgcgatcaa gacctatttt ttcaattaca tgtgaatatg tgtaactttc aagtccgggc
     6841 cttagttcat ttttaaagtt cttcttttct atacgctcaa gaaatagccg tttattcatt
     6901 ttctcgagtt ctttctggga ggcttcgagt tcagactcct tgcttttgag ttgaagcaat
     6961 atttgctctt tttcctcttg ttctaattgt gacttttcaa taagatcgtt cacgatctta
     7021 tttcgctcct ctatgacttt ctctatttct tgtttctcgc cctcaatctt tctcttggtg
     7081 tcgtcaattc ctgtacctct gcttggatat gtttgaaggt attcgcgcat agatgagtag
     7141 ccctcatcta ggttccttag tttttcacca aacccgcttt ccatttttac caatagctgc
     7201 gctatgtctt tcgtaaagtt gtaggtgttg tcataaaatg tattgctagt atccgtggcc
     7261 ttgaagtaaa aaagagccgc taacgcgaca gaaaataaag ccaacaagag tgatagcaag
     7321 gttggaaagt caactgtcaa ggcgattggt gttaaatata ccttatatga aattatgcct
     7381 actgctgtaa gtgtagaaat tgctttgaca gagtcccaga ttatttcctt tattagtctt
     7441 aatgtttcgt caagcatata tgcgtcctgg tttggcataa ctattaatta tgcgacacgt
     7501 gtgtcgcgat atatgtcgca taaacttcct agtcgtcggc taagtccatg cctgccttgt
     7561 aggtgttcgc agcaaggcga cagcacctat aggaaagcgc gacatggaga acagtaagcc
     7621 ctagctactg gcgaaatgct ggcaggcttg agccagaagc tagttcattc atgcttaggc
     7681 tgtccatggc ggcagctaaa acagaattac agtcagctct tgcctttgat ttatcaacca
     7741 cagaaacatc acccgcgtgc caaatcccca tcagaaggcc gagcgtaggt gttgcgtagg
     7801 gggacgagcc gcatggatgc ggcgagaggc ttaaagggcc agggatggcc cttgtaagcc
     7861 ggcccccgga gcagcaccgg agggagggaa gttgagcgaa gcgaaacccg gatgcagggg
     7921 cgtgtttctt ttgcttactt ttctttgcac gcgcaaagaa aagtgaggcg cccgaggggg
     7981 gcgcaacacg aaccatcagt acacgcgatc taatgccgaa tacggaggag ggcgcaaagc
     8041 atcgcggcca aggactaggc gtccccgccg ctcctacaaa agcgcgccct caactccaca
     8101 attccccaac cggcgtatcc cccgaatacc gcgtcgccac ctgcgcctgc agcaactcag
     8161 cggttgccag cgcatcggtc aacgcatggt gcgcctggta catcggcaag ttgtagcgca
     8221 ggcgactatc agccaaacga atcgacaccg gttgccggcc caacaaccgg cccagccagc
     8281 ccggctggcg cttgggatac aggcgcgcct ccagctgcat ggtgtcgata actgggaact
     8341 gtaaaccctc acccaattgc tggcgcagcg cctggtcgag aaagctgcgc tcgatggtgc
     8401 ggtaatgcac caccatcacc ttgccggcca tggcggtcag cagctcgtcg agtatctgcc
     8461 ccagacgcgg ggcatggcgg atatccgagt gggtgatgtg gtggaaggtc accgattcgc
     8521 cgctcagctc gcaggccggt ttgaccaccc agtagtgcgc cgcgccgcag cggatgcgtt
     8581 gcaggttgaa gggcagcagg ccgaggctga cgatggcgtg ctggttggcg tccaggccgg
     8641 tggtttccac gtccagggcc agcagtggca cttgctccaa cggggtgtca gcggccacac
     8701 agccggcctg gtagaaggct tgcaagcgtg agtccgtcgc gctggcggcg agttcggcaa
     8761 agcgtgcggc ccagtctagc ggcgtggcga gcttcgttgt cacaggcttt tacccggttg
     8821 cgcggggtaa cggaaacgca gaaatttctg cgcgttgcta atcacctgaa aggcttcctt
     8881 gaggttgtgc cgatcactgg cggagacttt ttccggctcg atgtagttgt tcggctgcgc
     8941 gccgtcggcg gcggccatgg cctggtggcg ggcgcgtacc aggctgagaa attccagggc
     9001 gtagcgcagc tggctgatgg cttcgggctg tagcaacttg gttttgccga tggcgtcgag
     9061 gcgctccagg gagttttgcg ccttgctgcc gcaggccagg gcgtgcacgc ggatcaggtc
     9121 ggtgagtggc gcggtgccac ggcctttgag gttgatgatg ttcttctgct ggccgtcggt
     9181 ttccatcaca aaggtgcgga agaaccccag cggcggcgtg cggttgagag cgttgcgcgc
     9241 cagggcggcg aggaaggccg ggttactgct ggccttctgc gcgagcagat ctttgaggtt
     9301 ttccaccagt tcgaactcgc catacacgcc gtcgaggtca aagaagatgc agctgtgcag
     9361 cagggtttgc ggtttgggtt tgtcgatcca ctggttgaag tagccgcgcc aaacgctcag
     9421 gggctgccgc cactgggcgt tgctggccat gatgccgccc ttgcagtagc tgtagccgca
     9481 ggcggccagg ccgtcgctga cgaactgggc gagggcggcg aagtaggcgt cgtgctgtgc
     9541 tggatcgaag cggtcatcga ggaccagggc gttgtcctga tcggtgacca gcagctgttc
     9601 gtcgcgggcc atggagccgg cgaccataaa gcagtagggc accggcggcg ggccgagttt
     9661 ctgctcagcc aattccagca ggcgctgggt aaaggcgcgg ccgatgccgg ccatggcgct
     9721 gccgaccatg tgcgcggtgg cctgctcgtt gaccatgcgc acgaaggtgc cacgcaggtc
     9781 tgccagcagg ctctgcaggc cggcgacatt cggctggttg aagatcgagt tgaccaggta
     9841 caggctgctt tgcgactcgt agcggatgat gtcgctgagg ttgatcaggc caatcggccg
     9901 gcgccgctgc accaccggca ggtgatggat gttgtggcgc agcatgcaca gcatggcctc
     9961 gaatacgctg tcatcggcct gcacggtgaa gggctcgggg gtcatcacct ggctgaccgc
    10021 tgtgctgctc gcaagcccgg cggcgagcac gcgggtacgc aggtcgcggt cggtgaggat
    10081 gccgaccatt gccgcgccgg gcgcatcgcc gtcgacaatc accagtgagg acacgctctg
    10141 ctcggtcatc agctgggcgg cctgttgcac gggtgtatcc aggctgacgc agaccggcgg
    10201 gcgcgagatc agcttgcgca ccttgagctg gatcagctcg ctggcctggc cctggctctc
    10261 caccgcggat ttcagccgcg actgaccttc ggcctcgacg aattcggcaa agccgtcgtg
    10321 ctcttcgcac agctgggcga acagggtggc ggggataaag tagatcaggc tgtcttcaat
    10381 cgcccgcgcc ggaaagcgca ccttgttgct gcgcagcagg ccggcctggc cgaagatatc
    10441 gccctcgctc aggcggttgt acagctcgcc gttgcgccgg tagatttcca ccgcgccgct
    10501 gcgtacatag tgcagctcgt gaatcgccgc gccgtactgg agaatgtcgc tgccggcttt
    10561 gaagtaggtg acttcgacct gccgggcgat ggcgtcgagg ttgtcctcgg gcagcgcatc
    10621 gaaaggcggg aagcgctgca ggtggtcgcg gatctccagt aactcgattt gcatggggtg
    10681 cctcgcagtg acaggcacta gaggatacgg cgattgccgg gtgctgtccg ccttgctggc
    10741 gttactgggt gtggcggctg ggtgagaggg tgctgagtcg ctagctctct aaggctatgc
    10801 tggggtgacg ggtgccgcga tgctcagctt ttgtaggagc gggcttgccc gcgattgcgg
    10861 tcaattgaaa aacatcgcgg ccaaggccgc tcctactggt tgtgtgcaac ctgtcattga
    10921 gggcattgct ttttaacgac ctgctttcaa ggagtggcta tgcaaggggc gggattctgg
    10981 gtggtgctgg cgctgctgtt ggcgctcgct ggtttgacga tttattacta caaccgcctg
    11041 gtgtttaacc gcgcgcgggt ggccgaggcc tggagcggta tcgacgtgca gctcaagcgc
    11101 cgcgccagcc tgattccggc gttggtcgag tgcgtgcgcg ggtacatgaa ctacgagcag
    11161 gacaccctgc aggcgttggt gcaggagcgt ctgcgcgcgg tgcagctggc cgattcgcca
    11221 ctgggcgagc gggcaccggt ggaggcgcat ctgggcactc aactgcgcca gctgtttgcc
    11281 ctggtcgagg attacccggc gctcaaggcc gacggccagt tcctcgacct gcagcgcaac
    11341 atcactgacg tcgagaacca tatccagatg gcccggcgtt actacaacgg cgcagtgcgc
    11401 gagctgaatg tgctggttga gtcggtgccg agcaatctgg tggcgcggcc ctttgccttt
    11461 acccaggcgg agtacttcag cctggatgac ccgcgtgacg cccagtcgcc aaaaatggag
    11521 ttttgacatg ttgcgagtgc tgcgttttgc cctgttgagc ctgtgcctgc tgccgctgtg
    11581 ggccgcagcc gccgaggtga ttgaggattt taccgtgacc ctgcaggtgc aggcggatgg
    11641 caacctgctg gtgaccgagc ggattaccgt gcaggccgag ggcgagcaga tccgccgcgg
    11701 catttatcgc gatctgccgg tcagctacag catggcgctg ggtttgcaga agagcggccc
    11761 gatcagcctg ttgggcgtga cccgtgacgg ccaggcggag cgcgtgacca ccgagcgcaa
    11821 cggcgcctgg gtgcgttatt acattggttc ggcggaccat ctgttgcagc ccgggcgcta
    11881 tcaatacgag ctgcgttatc gggtcgagcg gcagcttctg catcattcca ctaccgacga
    11941 gttgtactgg aatgtcaccg gtaatcagtg ggtgttcccg attctgcagg cctcggtcga
    12001 ggtgcagttg ccgccgggcg cgcggattgg ccagctggcg gcctataccg gggcccatgg
    12061 cgaacagggc aaggcgttcg aggtggtcga gcagcgcgac gactacctgc gactggtcac
    12121 cacgcaagcc ttgtcggcct atcacggcct gacggtggcc gtggattggc cggcgggcct
    12181 ggtggcgcgg ccgggtatag cgcagcgcct gggttatctg ctgctggata acctcggtct
    12241 gtgcctgggc gcgctgctgc tggtcggctt ggtgattttt tatctgcgcg cctgggaccg
    12301 ggtcggccgt gacccgcaaa agggcctgat catctcgctg ttcgaggccc cgcagggcat
    12361 gagcgcggtg caggccggct acctctggca tcgcgggttg aaggggccgt atcaggcggc
    12421 gcgggcgttc agtgtatggc tcaccgatat ggcgatccgc aagcatctgc atctcgaaga
    12481 caagccgcgc ggcggcggtt ttaccctggc ccgtggcacg ggtgagcgtg acgatttcag
    12541 cgagggtgat cgcgacctgc gcaagcgcct gttcccggcc aataaggaag gcatcgccct
    12601 ggagattggc agcgattatg agccgcgcct ggccgatgcc gtggccgggc tgaccactca
    12661 gctgcaaacc cagggcaagg cctggttcag caacaaccgt ggcatttggg cctggggcct
    12721 ggtgtgggcg ttggtcgcgt gtctggtaat ggtgttttcc ggggcgcagg gtgaggacga
    12781 tatagccacc ggcttggccg gggtgatgtt taccctgggc tttggcgtgc cgtcgctatt
    12841 tgtgctgtac atggcctgga atcagcccag tttgggcaag cagatcggtc tgggtctggc
    12901 ggggttgatg tttgtctggc cggtgccgat tggtttgtgg atgctcagtg atgcggcgtc
    12961 ggtgccggct ttgctgctgc tctgcgtgta tgtgctggtg gtggtgctgt tttattactt
    13021 gctgccggcg ccgtcgattg aggggcggcg tttgcttgat cagctggaag gctatcgcga
    13081 ctacctgcaa ttggcagaaa gcgaggccct ggccctggcc ggcaatgccc cggcgatgag
    13141 catcgcgctg tacgagaaac acctgcccta cgccatggcc ctgggcgtgg aggacaagtg
    13201 gagcgcgcgc tttagcacgg ccctggccag cggcctgatc gatccggcgc agcgtgacta
    13261 tcaacccgac tggtatcaca gtcgcagctc attcagcact ccgctggcga tgagcgccgc
    13321 cctggctgcc ggcctaagca gcgccacggc gctggcctca tcgccgccgt cgagtagctc
    13381 gtcaggcggc ggcggctcat ccggcggtgg ctcgtcaggt ggtggcggtg gtggcggggg
    13441 cggcggcggt tggtagagcg cagttcagct aggtggcgga cgcttttgta ggagcggggg
    13501 gacgcccagt tattgcccgc gatcacggtc aatgcaacaa catcgcggcc aaggctgctc
    13561 cggccatcgg cttgtaggag cggg
//
LOCUS       DASRUM010000294        92576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1337988, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000294 DASRUM010000000
VERSION     DASRUM010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 92576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92576
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337988"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1528)
                     /gene="gatA"
                     /locus_tag="VFF22_19520"
     CDS             complement(77..1528)
                     /gene="gatA"
                     /locus_tag="VFF22_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HZX19045.1"
                     /translation="MHQFTLAEIARGLADKQFSSEELTRSLLARIAQLDPQLNSFITV
                     TEELALSQATAADARRANGENGALLGAPIGHKDLFCTQDVLTSCASKILTGFKAPYNA
                     TVVEKLAAAGTVTLGKLNMDEFAMGSANESSHYGAVKNPWDLTRVPGGSSGGSAAAVA
                     ARLLPAATGTDTGGSIRQPAALTNLTGIKPTYGRVSRWGMIAYASSLDQGGPLARTAE
                     DCALMLQAMAGFDTKDSTSVDQPVDDYLAALTLPLAGLRIGLPKEYFGAGLDARIGEK
                     VMAVVEELKKLGATIKEISLPNMQHAIPAYYVIAPAEASSNLSRFDGVRFGHRCENPV
                     NLEDLYKRSRGEGFGAEVKRRIMVGTYALSAGYYDAYYLKAQKIRRLIKNDFVAAFND
                     VDVILGPTTPNLAWKLGEKNNDPVAAYLEDIYTITANLAGIPGLSMPAGFVDGLPVGV
                     QLLGNYFQEGRLLNVAHQYQQVTDWHTHAPSGF"
     gene            complement(1544..1831)
                     /gene="gatC"
                     /locus_tag="VFF22_19525"
     CDS             complement(1544..1831)
                     /gene="gatC"
                     /locus_tag="VFF22_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZX19046.1"
                     /translation="MALERSDVEKIAHLARLGLNEADIPGTTETLNNILGLIDQMQAV
                     NTDGIEPLAHPLEATQRLRADQVTERNQREAYQAIAPAVENGLYLVPKVIE"
     gene            2098..3135
                     /gene="mreB"
                     /locus_tag="VFF22_19530"
     CDS             2098..3135
                     /gene="mreB"
                     /locus_tag="VFF22_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HZX19047.1"
                     /translation="MFKKLRGMFSSDLSIDLGTANTLIYVRERGIVLNEPSVVAIRTH
                     GNQKSVVAVGTEAKRMLGRTPGNIAAIRPMKDGVIADFSVCEKMLQYFINKVHENSFL
                     QPSPRVLICVPCKSTQVERRAIRESALGAGAREVFLIEEPMAAAIGAGLPVEEARGSM
                     VVDIGGGTTEIALISLNGVVYAESVRVGGDRFDEAIITYVRRNYGSLIGESTAERIKQ
                     EIGTAYAGGELREVDVRGRNLAEGVPRSFTLNSNEVLEALQESLATIVQAVKSALEQS
                     PPELASDIAERGLVLTGGGALLRDLDKLLSQETGLPVIVAEDPLTCVARGGGKALEMM
                     DRHTMDLLSSE"
     gene            3223..4182
                     /gene="mreC"
                     /locus_tag="VFF22_19535"
     CDS             3223..4182
                     /gene="mreC"
                     /locus_tag="VFF22_19535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZX19048.1"
                     /translation="MKPLFAKGPSLGVRLLVFTVLSAALMVVDARFTLLHPLRAQLGL
                     IVEPVYWVGRLPVRVWESATQELSTRNELAAENEKLKAEQLMMQRRLQKLAALTEQNV
                     RLRELLNSAALVDDDVLATELIGIDPNPFTHRILIDKGEKDGVVLGQPVLDARGLMGQ
                     VVEVMPYTSRVLLLTDTTHSIPVQVNRNGLRAIASGTGNPERLELRHVADTADIKEGD
                     LLVSSGLGQRFPAGYPVAMVSEVIHDSGQPFAIVRAVPTATLNRSRYMLLVFTDPRTP
                     EQRATESAQAQEALDREILQQAQPEQPAVPAPAAPANPPEATQ"
     gene            4182..4667
                     /gene="mreD"
                     /locus_tag="VFF22_19540"
     CDS             4182..4667
                     /gene="mreD"
                     /locus_tag="VFF22_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HZX19049.1"
                     /translation="MAARSSNIWVVWLTLAVALLLSVSSMPKFMELGRPLWLALFLTY
                     WVLAVPHRVGMTTAWVIGLLADVLNGTLLGQNALILTLITFLVLTLHQRLRMFPMWQQ
                     STVLLVVFGLAQLVQLWLNALTGSRPPTLTFVLPALVSALLWPWVCTLLRGLHQRLGV
                     N"
     gene            4713..5333
                     /locus_tag="VFF22_19545"
     CDS             4713..5333
                     /locus_tag="VFF22_19545"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HZX19050.1"
                     /translation="MATLYLASGSPRRRELLTQIAVPFLTQIAPIDENALPGESPIAY
                     VERLALSKAQAGLAALTDTADAVVLGADTAVVLDGRILGKPTDRADALATLSDLSGRT
                     HEVLTAVALVSRERQVSRVVTSQVTFRALSQAEIEAYWASGEPQDKAGCYGIQGLAAV
                     FVSQLQGSYSAVVGLPLCETAALLAEFAIPCWQSLPVHSQEVAGRG"
     gene            5395..6852
                     /gene="rng"
                     /locus_tag="VFF22_19550"
     CDS             5395..6852
                     /gene="rng"
                     /locus_tag="VFF22_19550"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HZX19051.1"
                     /translation="MSEEILINITPMESRVAVVENGVLQEVHVERTQKRGIVGNIYKG
                     KVVRVLPGMQAAFIDIGLERAAFIHAAEISTREGSAVESISALVHEGQSLVVQVTKDP
                     IGSKGARLTTQLSIPSRYLVYMPRTAHVGISLKIEDEAERERLKQVVADCVAAEGIEE
                     AGGFILRTAADGAGADEILADIRYLRRLWEQIATQMKVGPTPLVIYEDLSLALRTLRD
                     LVNPKIEKIRVDSRETFGKITQFVDELMPEIADRLEHYPGERPIFDLYGVEDEIQKAL
                     ERKVPLKSGGYLIIDPAEAMSTIDVNTGAFVGHRTLEETIFKTNLEAATAIARQLRLR
                     NIGGIIIIDFIDMEDEEHQRQVLRTLEKQLERDHAKTNIIGITELGLVQMTRKRTRES
                     LEQVLCEPCSACQGRGKLKTAETTCYEIFREILREARAYQPEGYRVLANQKVVDRLLD
                     EESGNVADLEAFIGRTIKFQVETMYSQEQYDVVLL"
     gene            6884..10717
                     /locus_tag="VFF22_19555"
     CDS             6884..10717
                     /locus_tag="VFF22_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HZX19052.1"
                     /translation="MAALARWVTLLLRTALGLCALTLVLAALYVSLGRELVPLVAEYR
                     LEAEDKARQALAMPVQIGELEGLWQGFSPVLTAHDVALGEGAGALQLDQVRVVPDVLA
                     SLLARQLRIARLELDGVQLVVQQAEDGSWSLKGLPQRNEPAPLDLAQVLTQSQMLKRL
                     SLFNSQVTMQAFDQPARTLTYVNLTLRNGSARQRLDGRLLLPDGQPLALQLRTHINAK
                     HWQDAQAELYLSLPQSDWAAWVPTSLLAQWRLEQLQLGGEVWLSWANGGVQRAVSRLH
                     VPQLKAGYAERESVALNNLALNAYFTRTEQGFKLLLDDLAFSRGEQRWGDVRLALTQQ
                     AESAEVQEQWLLSADRLDLAPLQPLVAGLAPLPDKGLALLEQLKPHGALRNLQLDYRP
                     RLRDGKRLQFSANLERIGFAAYLGSPAAENVSGSISGDLGQGELRLDSEDFSLHLDTL
                     FPKPWVYQKANALLTWQIDEHAFTLRSPYLRVQGEEGPVAGDFLIRLRVDPTQEDYMD
                     LRVGLRNGDARFTEKYLPSRAPGMSADLDAWLKSAIRGGAVDEGYFQYQGSLNKGAEN
                     AARSISLFFAVHDAELAFQPGWPALREGRGRVLIEDTGVRVELAEGRILDSQVSDAQA
                     LIATALPGQPPRLLLTSELSSNVEDALHILQQAPIGTAETFAGWTGAGALAGKLKLDL
                     PLRKGLAPAVVVDFATESAQLSLPNPPLQFSQLQGAFRYNTASGLSAPDIRAQVLGHA
                     VRGKALAEGARGKARSRIEASGEIAVKELSTWLGVTQPLPISGMLPYRLNLILEGADS
                     QLRVSSSLKGATVALPAPFGKAAAEARATQWRMSLGGREQRYWLDYSDLLSLTFAASP
                     GQFNQGRGEIRLADGPASLPTSQGLRVRGRVAELDWSAWQAVLQPYASVPIGDAQRLF
                     KDAQLRIGRFSGFGISLDTLEVAVQREAATWAVSLDSPQLKGQVGLPDARELPIALTL
                     DYLRLPAAEKKIAGEAVVDAPDPLASFDPSRFPALDVRIAEVYQGIEPLGSWSLKARP
                     VSAGVHFNELDLNLKGLKIGGRATWLNAANGVRTSYKGRMEGADLADVLIAWGFAPTA
                     SSRDFHMDVEGDWPGSPAWFGLKRFSGTLDASLRKGQFSEVEGPASALRVFGLLNFNS
                     IGRRLRLDFSDLLGKGLSYDRVKGLLVADNGVFVTREPITLEGPSSNLELNGTLDMAN
                     DRIDAKLLVTLPVTNNLPLAALIVGAPAIGGALFIADKLLGDRVARFASVQYDVKGAW
                     QNPDISFDKPFEKPQ"
     gene            10803..11672
                     /locus_tag="VFF22_19560"
     CDS             10803..11672
                     /locus_tag="VFF22_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZX19053.1"
                     /translation="MTFAVIQMVSQADVLANLIQARQLLEQAAAQGARLAVLPENFAA
                     MGRRDQAALGRAEALGEGPILPWLQQAARDLDLWIVAGTLPLPPDGQPDAKAHACSLL
                     IDNAGQRVARYDKLHLFDVDVSDSHGRYRESDDYAFGAQVVVADTPVGRLGMTVCYDL
                     RFPELYSALRAAGAELISAPAAFTAVTGAAHWQILTRARAIETQCYVLAAGQGGTHPG
                     GRETFGHSALVDPWGRVLAELPQGVGVLLAERDATEQAAIRQRMPVARHKRFFAAAEA
                     RLPGAIVINEIEQ"
     gene            11669..13126
                     /gene="tldD"
                     /locus_tag="VFF22_19565"
     CDS             11669..13126
                     /gene="tldD"
                     /locus_tag="VFF22_19565"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HZX19054.1"
                     /translation="MNSMLVSVSEHLLGPGGLTIEHLPAVLADLAGPGIDAADLYFQS
                     QVSETWALEDGIVKEGSFNLDQGVGVRAQSGEKTGFAYSNAITADALSQAARAARSIA
                     RAGQNGRVQAFSSPLVKPLYAQDNPLDVLGRAEKVELLKRIDVATRALDPRIKQVSVS
                     LAGVWDRVLVAANDGSLGADIRPLVRFNVSVIVEQNGRRERGSQGGGGRTDYRYFLEQ
                     TSNNEERAMGYAREALRQALVNLEAIPAPAGTMPVVMGAGWSGVLLHEAVGHGLEGDF
                     NRKGSSAYSGRMGEQVASSLCTIVDDGTLAERRGSLSMDDEGTQTQCTTLIENGVLKG
                     YMQDKLNARLMGVACTGNGRRESYAHLPMPRMTNTYMLAGESDPEEIIRSVKKGIYCA
                     NLGGGQVDITSGKFVFSTSEAYLIEDGKITAPVKGATLIGNGPEAMSKVSMVGNDLAL
                     DSGVGTCGKDGQSVPVGVGQPTLKIDAITVGGTGS"
     gene            complement(13175..13696)
                     /gene="yjgA"
                     /locus_tag="VFF22_19570"
     CDS             complement(13175..13696)
                     /gene="yjgA"
                     /locus_tag="VFF22_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HZX19055.1"
                     /translation="MSEYLDDDFSGEKSKTQIKREFHALQDLGQRLTTLKLDLLNKLP
                     LTDELRRALAEAPKHTANAAKKRHVQFIGRLMRDQDIPAILTLLDQLDASTRQYNERF
                     HNLERWRDRLLNGDDDTLEKFVVEYPDADRQHLRQLIRQGQHELAQNKAPAASRKIFK
                     YIRELDETQRGLR"
     gene            13800..15146
                     /gene="pmbA"
                     /locus_tag="VFF22_19575"
     CDS             13800..15146
                     /gene="pmbA"
                     /locus_tag="VFF22_19575"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003171795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HZX19056.1"
                     /translation="MSAVDIVGPQAVPHLQAQVEQIIAEAKKQGASACEVAVSVEQGL
                     STTVRQGDVETVEFNRDQGFGITLYVGQRKGSASTSASGEAAIRETVAAALAIAKHAS
                     EDECAGLADAALMARELPQLDLYHAWSITPEQAIEQALSCEAAAFAADQRIKNADGTT
                     LNTHQGCRVYGNSHGFISGYASTRHSLSCVMIAEDGGQMQRDYWYDVNRQGELLADAS
                     SIGRRAAERAVSRLGARPVPTCEVPVLFAAELAGGLFGSFLAAISGGNLYRKSSFLEG
                     TLGQQLFPTWLTLDERPHIPRALGSAAFDGDGLATYAKSFVERGELLSYVLGTYSGRK
                     LGMPSTANSGGVHNLFVSHGDEDQKALLKRMGRGLLVTELMGQGLNMVTGDYSRGAGG
                     YWVENGEIQFPVQEVTIAGNLRDMFKQIVAVGNDLELRSNIRTGSVLIERMTVAGS"
     gene            complement(15214..15486)
                     /locus_tag="VFF22_19580"
     CDS             complement(15214..15486)
                     /locus_tag="VFF22_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HZX19057.1"
                     /translation="MPACEIIIINKLGLHARAAAKFVGVAGRFPCQVKVGRTPESLVD
                     GKSIMAVMMLAAGKGTPVHLHTEGEQDSEALQALSELINNYFDEGE"
     gene            complement(15499..16356)
                     /gene="rapZ"
                     /locus_tag="VFF22_19585"
     CDS             complement(15499..16356)
                     /gene="rapZ"
                     /locus_tag="VFF22_19585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HZX19058.1"
                     /translation="MRLIIVSGRSGSGKSTALDVLEDNGFYCIDNLPAGLLPELAERA
                     LLHTELLHPQVAVSIDARNLPSHLKRFPELLDEVRARHIKCDVIYLDADEETLLKRFS
                     ETRRRHPLTNESRSLAEAIADESLLLGPIIDLADLKIDTTHLNLYQLRDSLKLRLLNQ
                     PEPGTAFLLESFGFKRGMPVDADLVFDVRCLPNPYWKPDLRDFSGLDQPVVDYLSAQA
                     DVEDMYQDILAYLTKWLPRFAASNRAYVTVAIGCTGGHHRSVYLANRLGETLKPILKN
                     VQTRHRDLS"
     gene            complement(16359..16832)
                     /gene="ptsN"
                     /locus_tag="VFF22_19590"
     CDS             complement(16359..16832)
                     /gene="ptsN"
                     /locus_tag="VFF22_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HZX19059.1"
                     /translation="MIRLETILTPGRSLVNVPGGSKKRVLEQIANLVARELPDLDGQD
                     IFESLIAREKLGSTGFGNGIAIPHCRLPGCTAPISALLRLDAAVDFDAIDGAPVDLLF
                     VLLVPEAATDAHLELLRQIASMLDRSDVRASLRQAESSEALYQVVVDVQSQLNRA"
     gene            complement(16848..17156)
                     /gene="raiA"
                     /locus_tag="VFF22_19595"
     CDS             complement(16848..17156)
                     /gene="raiA"
                     /locus_tag="VFF22_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HZX19060.1"
                     /translation="MQVNISGHQLDVTDALRDYIAEKLGRLERHFDKITNVQVTMEVE
                     KLKQKIEATLHIAGGEVVANAEHEDMYAAIDLLTDKLDRQLIKHKEKQLERQQGAMAR
                     "
     gene            complement(17350..18864)
                     /locus_tag="VFF22_19600"
     CDS             complement(17350..18864)
                     /locus_tag="VFF22_19600"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HZX19061.1"
                     /translation="MKPSLVLRMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESN
                     PMLERQEDGDDFDNSDPMADGAESASPSEQPEAPYQETNYQEATQTVDNLEEGDWGER
                     IPNELPVDTAWEDIYQTSASSLPSSTNDDDEWDFTTRTSSGESLQSHLLWQLNLAPMS
                     DKDRLIGVTLIDCINQQGYLEETLEEVVEAFDPELDIELDEVEAVLHRIQQFEPTGIA
                     ARNLGECLLLQLRQLPAKTPWLSEAQRLASDYLDLLGSRDYSQLMRRMKLKEDELRQV
                     IDLIQSLNPRPGSQIESSEPEYVVPDVIVRKHNERWLVELNQEAMPRLRVNAQYAGFV
                     KRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGDEAMKPL
                     VLHDIAEAVGMHESTISRVTTQKFMHTPRGIYELKYFFSSHVSTSEGGECSSTAIRAI
                     IKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAKYRESLGIAPSSERKRLI"
     gene            complement(19003..19728)
                     /gene="lptB"
                     /locus_tag="VFF22_19605"
     CDS             complement(19003..19728)
                     /gene="lptB"
                     /locus_tag="VFF22_19605"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HZX19062.1"
                     /translation="MATLKAQHLAKSYKGRQVVRDVSLSIDSGQIVGLLGPNGAGKTT
                     CFYMIVGLVQADQGRVMIDDLDVSHQPMHGRARAGIGYLPQEASIFRKLSVADNIMAI
                     LETRKDLDSAARRKELEGLLQEFHISHIRDNLGMSLSGGERRRVEIARALATNPKFIL
                     LDEPFAGVDPISVGDIKQIIHHLKAKGIGVLITDHNVRETLDICETAYIVSDGQLIAE
                     GDAETILANQTVKEVYLGHEFRL"
     gene            complement(19731..20273)
                     /gene="lptA"
                     /locus_tag="VFF22_19610"
     CDS             complement(19731..20273)
                     /gene="lptA"
                     /locus_tag="VFF22_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HZX19063.1"
                     /translation="MRLVNTLPLLLSLSAALGSVSAWALPSDREQPIRIQADSAELDD
                     KQGVAVYRGDVVITQGTMKVTGDTVTITQNAQGDVEVFTSVGKPAYYEQLPSADKQIV
                     KAYGLTIQYFMANDRVVLIDQAKVIQEGNTFEGEKIVYDTQRQIVNAGRATGTNITAP
                     RPRIDMVIQPKNKPADQQAQ"
     gene            complement(20260..20829)
                     /gene="lptC"
                     /locus_tag="VFF22_19615"
     CDS             complement(20260..20829)
                     /gene="lptC"
                     /locus_tag="VFF22_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003302839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HZX19064.1"
                     /translation="MPRKLFNIALFAFAALLVAALGYWNLSPEGFNEQSRGAGTDNTI
                     DFYATNTYTVQYQADGKLHYELTADKVEHVKASDITLMTSPNMNLFRGTELPWEIRSE
                     RGEVSPGGVEVELIDKVRVERTDAKGRPTILTTSRLTVFPEKEYAQTQQAVRIDAANG
                     VTTAKGMKAYMNDGRMLLLSNVRGQHEAR"
     gene            complement(20837..21376)
                     /locus_tag="VFF22_19620"
     CDS             complement(20837..21376)
                     /locus_tag="VFF22_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003302838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZX19065.1"
                     /translation="MTTDQINSDLLQRAQAVKLAIFDVDGVLTDGKLYFLVDGSEFKT
                     FNTLDGHGIKMLINSCVRTAIISGRKTPVVERRAQNLGIQHLYQGREDKLVVLDELLG
                     ELGLDYAQVAYLGDDLPDLPVIRRVGLGMAVASADGFVRQHAHGVTRARGGEGAAREF
                     CELIMHAQGTLEAAQAAYL"
     gene            complement(21376..22350)
                     /locus_tag="VFF22_19625"
     CDS             complement(21376..22350)
                     /locus_tag="VFF22_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003188589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HZX19066.1"
                     /translation="MSQSTDLIQSAQRTIRLELEAIQDLLARINGDFVRACELILASK
                     GRVVVVGMGKSGHVGNKIAATLASTGTTAFFVHPAEASHGDMGMITRDDVVLALSNSG
                     STAEIVTLLPLIKRLGIRLISMTGNPESPLAKAAEVNLDARVSQEACPLNLAPTSSTT
                     VSLVLGDALAIALLEARGFTAEDFAFSHPGGALGRRLLLKVENVMHAGDSLPCVKRGT
                     SLRDALLEMTQKGLGMTVVTEDDGRLAGIFTDGDLRRTLDRNIDVRQTTIDDVMTAHG
                     KTARAEMLAAEALKIMEDHKISSLVVIDDNDLPIGALNMHDLLRAGVM"
     gene            22597..23406
                     /locus_tag="VFF22_19630"
     CDS             22597..23406
                     /locus_tag="VFF22_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZX19067.1"
                     /translation="MSAENPYAVELKNVSFKRGERDIFKNIDIRIPRGKVTGIMGPSG
                     CGKTTLLRLIAAELKPSQGEVWVNGLNLPTLSRSELFDMRKQMGVLFQSGALFTDLDV
                     FENVAFPLRVHTTLPEEMIRDIVLMKLQAVGLRGALELMPDELSGGMKRRVALARAIA
                     LDPQILMYDEPFVGQDPIAMGVLVRLIRLLTDALGITSIVVSHDLAETASIADYIYIV
                     GDTQVLGQGTPAELMASDDPRVRQFMQGIPDGPVPFHFPAANYRDDLLGER"
     gene            23406..24203
                     /gene="mlaE"
                     /locus_tag="VFF22_19635"
     CDS             23406..24203
                     /gene="mlaE"
                     /locus_tag="VFF22_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HZX19068.1"
                     /translation="MRKTSVLERVRLFGRAGIDVVATLGRSGLFLLRALFGRGSTGNS
                     FQLLIKQLYSVGVMSLAIIVVSGIFIGMVLSLQGFSILAKYGSEQAVGQMVALTLLRE
                     LGPVVTALLFAGRAGSALTAEIGNMKSTEQLSSLAMIGVDPLKYIVAPRLWAGFISMP
                     LLAMIFSVVGIWGGAMVAVDWLGVYEGSFWANMQSSVSFREDVLNGVIKSVVFAFVVT
                     WIAVFQGYDCEPTSEGISRATTKTVVYASLAVLGLDFILTALMFGDF"
     gene            24203..24673
                     /gene="mlaD"
                     /locus_tag="VFF22_19640"
     CDS             24203..24673
                     /gene="mlaD"
                     /locus_tag="VFF22_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HZX19069.1"
                     /translation="MQNRTLEIGVGLFLLAGLLALLLLALRVSGLTVGSSDNTYKVYA
                     HFENIAGLTVRSKVTMAGVTIGKVTAIDLDRNSYMGRVTMVLDDAVDNLPADSTASIL
                     TAGLLGEKYIGLSVGGEEEVLRDGGTIHDTQSSLVLEDLIGKFLLNSVNKDQAN"
     gene            24688..25320
                     /locus_tag="VFF22_19645"
     CDS             24688..25320
                     /locus_tag="VFF22_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX19070.1"
                     /translation="MIKALRNGLFICLAAMSLPLLAAPAAHEVVQQTTTTLLADLKAN
                     KEQYRTDPGAFYTALNNILGPVVDVDGISRGVMTVRYSRQASPEQMQRFQENFKRSLM
                     QFYGNALLEYNNQDIRVLPVSGQQDPERTSVNMEIKDGKGVVYPLSYTMVNQGGTWKM
                     RNVVINGINVGKLFRDQFAQAMQDNRNDLDKVIDNWTETVAKARQGANAS"
     gene            25317..25625
                     /locus_tag="VFF22_19650"
     CDS             25317..25625
                     /locus_tag="VFF22_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZX19071.1"
                     /translation="MSQASIREASPGVFNLTGVLDYLSAPALREQGASLLKVSPAAAC
                     VLDCSGVVKSSSVGLALLLAFMRDASAAGKTLSVSNLPKDMREIAGVCGLLEIIPLQA
                     "
     gene            25747..25986
                     /locus_tag="VFF22_19655"
     CDS             25747..25986
                     /locus_tag="VFF22_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HZX19072.1"
                     /translation="MQALEVKSLLEAKLPDTQVEVEGEGCNFQLNLISDELAALSPVK
                     RQQQIYAHLNAWIADGSIHAVSMKFFSRADWAARS"
     gene            25999..27264
                     /gene="murA"
                     /locus_tag="VFF22_19660"
     CDS             25999..27264
                     /gene="murA"
                     /locus_tag="VFF22_19660"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZX19073.1"
                     /translation="MDKLIITGGVRLDGEIRISGAKNSALPILAATLLGDAPVTICNL
                     PHLHDITTMIELFGRMGIEPIIDEKLSVEVDARAIKTLVAHYELVKTMRASILVLGPM
                     VARFGYAEVALPGGCAIGSRPVDLHIRGLEAMGAIIDVEAGYIKAKAPEGGLRGAHFF
                     FDTVSVTGTENIMMAATLAKGRSVLENAAREPEVVDLANCLIAMGAKISGAGTDTITI
                     DGVERLHGARFSVMPDRIETGTYLVAAAVTGGRVKVKDTDPTTLEAVLSKLQEAGAEI
                     TCGNDWIELNMHGKRPKAVNLRTAPYPAFPTDMQAQFIALNAIAEGTGTVIETVFENR
                     FMHVHEMIRMGAQIQVEGNTAIVTGVETLKGAPVMATDLRASASLVISALVAEGDTLI
                     DRIYHIDRGYECIEEKLQMLGAKIRRIPG"
     gene            27269..27904
                     /gene="hisG"
                     /locus_tag="VFF22_19665"
     CDS             27269..27904
                     /gene="hisG"
                     /locus_tag="VFF22_19665"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZX19074.1"
                     /translation="MLTIALSKGRILDDTLPLLAAAGIEPTENPDKSRKLIIPTTLDD
                     VRLLIVRATDVPTYVEHGAADLGVAGKDVLMEYGGQGLYEPLDLRIAQCKLMTAGAVG
                     APEPKGRLRVATKFVNVAKRYYAEQGRQVDIIKLYGSMELAPLVGLADKIIDVVDTGN
                     TLRANGLEAQELIAMISSRLIVNKASMKMQHARIQALIDTLREAVEARHPR"
     gene            28026..29336
                     /gene="hisD"
                     /locus_tag="VFF22_19670"
     CDS             28026..29336
                     /gene="hisD"
                     /locus_tag="VFF22_19670"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014821374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZX19075.1"
                     /translation="MTAPIAIRRLNAADQDFAQHLDHLLSWESVSDDAVNHRVLDIIK
                     AVRERGDAALVEFTKQFDGLDVASMADLILPRERLELALTRITPEQRSALEKAAERVR
                     SYHERQKQDSWTYTEADGTVLGQKVTPLDRAGLYVPGGKASYPSSVLMNAIPAKVAGV
                     PEVVIVVPTPRGEINELVLAAACIAGVDRVFTIGGAQAVAALAYGTESVPPVDKIVGP
                     GNIYVATAKRHVFGKVGIDMIAGPSEILVVCDGQTDPDWIAMDLFSQAEHDEDAQSIL
                     VSPDAAFLDRVAESIAKLLPTLERETIARTSLEGRGALILVADMAQAIEVANRIAPEH
                     LELSVENPEEYLPLIRHAGAIFMGRYTAEALGDYCAGPNHVLPTSGTARYSSPLGVYD
                     FQKRSSIIHCSAEGASELGKVASVLARGESLTAHARSAEYRIKN"
     gene            29396..30448
                     /gene="hisC"
                     /locus_tag="VFF22_19675"
     CDS             29396..30448
                     /gene="hisC"
                     /locus_tag="VFF22_19675"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZX19076.1"
                     /translation="MSKFWSPFVKDLVPYVPGEQPKLAKLVKLNTNENPYGPSPKAIA
                     AMQAELNDNLRLYPDPNSDRLKQAVADYYGVQTTQVFVGNGSDEVLAHAFHGLFQHGQ
                     PLLFPDISYSFYPVYCGLYGIDFVQVPLDEQFQIRVEDYARPNGGIIFPNPNAPTGCP
                     LALDAIERMLKANPDTVVLVDEAYIDFGGETAVSLVDKYPNLLVSQTLSKSRSLAGLR
                     VGIAVGHPDLIEALERIKNSFNSYPLDRMAIAGAAAAFEDKVYFEQTCRQVIASREAV
                     VAGLDKLGFEVLPSAANFVFARHPGKDAATLAAGLREQGVIVRHFKQARIAQFLRITI
                     GTPEQNQALLDALQSL"
     gene            complement(30568..31710)
                     /gene="algW"
                     /locus_tag="VFF22_19680"
     CDS             complement(30568..31710)
                     /gene="algW"
                     /locus_tag="VFF22_19680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase AlgW"
                     /protein_id="HZX19077.1"
                     /translation="MFKALRFLGWPLLVGVLSALLIMQYFPQWVGLPGQDVHVRQAAF
                     YSRMQEGPVSYAEAVTTASPAVANLYTTKFVKKPAHPLIEDPQFRKFFGGNLPQQRRM
                     ESSLGSAVIMSPEGYLLTNNHVVAGADQIVVALKDGRETLARLIGSDPETDLAVLKID
                     LQDLPFIILGRSDNIRIGDVTLAIGNPFGVGQTVTMGIISATGRNQLGLNTYEDFIQT
                     DAAINPGNSGGALVDAYGNLVGINTAIFSKSGGSQGIGFAIPVKLAVDVMQAIIEHGQ
                     VIRGWLGIEVQPLTPELAESFGMDGRPGIVVAGIYRDGPAQKAGLQPGDLILSIDGEA
                     AGDGRRSMNQVARAKPGQKINIDILRNGKPLQLTAEVGVRPPVNGS"
     gene            31824..32582
                     /locus_tag="VFF22_19685"
     CDS             31824..32582
                     /locus_tag="VFF22_19685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HZX19078.1"
                     /translation="MAIALATLVEEADRYLNAARISDYCPNGLQVEGRPQVRRIVSGV
                     TASQALVDAAVEAEADVLLVHHGYFWKGENPCITGMKRRRLQTLLANDISLLAYHLPL
                     DVHAEVGNNVQLAAQLGITVEGPLEPESSRTVGLLGSLAEAMSPRDFAHHVHQVLGRE
                     PLLVEGNEMIRRVGWCTGGGQGYIDQAIAAGVDLYLTGEASEQTFHSARENGVSFIAA
                     GHHATERYGVQALGDYLARRFAIEHLFIDCPNPI"
     gene            32725..33642
                     /gene="cysD"
                     /locus_tag="VFF22_19690"
                     /pseudo
     CDS             32725..33642
                     /gene="cysD"
                     /locus_tag="VFF22_19690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
     gene            33644..35545
                     /gene="cysN"
                     /locus_tag="VFF22_19695"
     CDS             33644..35545
                     /gene="cysN"
                     /locus_tag="VFF22_19695"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HZX19079.1"
                     /translation="MSHQSELISEDILAYLAQHERKELLRFLTCGNVDDGKSTLIGRL
                     LHDSKMIYEDHLDAITKDSKKVGTTGEDIDLALLVDGLQAEREQGITIDVAYRYFSTT
                     KRKFIIADTPGHEQYTRNMATGASNCDLAIILIDARYGVQTQTKRHSFIASLLGIKHI
                     VVAINKMDLKGFDQGVFEQIKADYLKFAEGIALKPSSLHFVPMSALKGDNVVNKSEQS
                     PWYTGQSLMEILETVEVAGDRNFTDLRFPVQYVNRPNLNFRGFAGTLASGIVRKGDEV
                     IALPSGKGSKVKSIVTFEGELEQAGPGQAITITLEDEIDVSRGDMLVHGDNRPQVVDS
                     FDAMLVWMAEEPMLPGKKYDIKRATSYVPGSIASITHRIDVNTLEEGAASSLQLNEIG
                     QVRIALDAPIALDGYTHNRTTGSFIVIDRLTNGTVGAGMIIAEPLGAQGSDGHHGKLA
                     HVSTEERAARFGQQPATVLFSGLSGAGKSTLAYAVERKLFDSGRAVYVLDGQNLRHDL
                     NKGLPQDRAGRTENWRRAAHVARQFNEAGLLTLAAFVAPDAEGREQAKALIGAERLIT
                     VYVQASPQVCAERDPQGLYAAGGDNIPGESFPYDIPLNADLVIDTQSVSVEEGVKQVL
                     ALLRERGAI"
     gene            complement(35629..36066)
                     /locus_tag="VFF22_19700"
     CDS             complement(35629..36066)
                     /locus_tag="VFF22_19700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HZX19080.1"
                     /translation="MEQTLTAWLLPAITLVAGIAIGFLIARLAPNAAPSRTQRQLDEM
                     QERFDAYQSEVVSHFNTTASMVKKLTQSYQDVQEHLSDGANRLALDELTRQRLLATLH
                     ADDASEKRERLTPPRNNEMPKDYAPKSANVPGMLDESYGLKNK"
     gene            36219..36848
                     /locus_tag="VFF22_19705"
     CDS             36219..36848
                     /locus_tag="VFF22_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZX19081.1"
                     /translation="MLTRETPLMIAGPSGQLEALWLDTPDVRGVALICHPNPVQGGTM
                     LNKVVSTLQRTARDSGLATLRFNYRGVGASAGSHDMASGEVDDAEAVANWLRAQHPEL
                     PLTLLGFSFGGFVAAALGARLEAQGVTLDKLFMVAPAVHRLSEETPPASNCPLILIQP
                     EDDEVIDPQVVYQWSAALGRAHELLKVAECGHFFHGKLTDLKDLLLPRL"
     gene            36877..38223
                     /locus_tag="VFF22_19710"
     CDS             36877..38223
                     /locus_tag="VFF22_19710"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZX19082.1"
                     /translation="MTTRIITGITTTGTPHLGNYAGAIRPAIVASRAADADSFYFLAD
                     YHALIKCDEPARIQRSRLEIAAAWLALGLDTDKATFYRQSDIPEIPELCWLLTCVAGK
                     GLLNRAHAYKASVDKNVEQGEDPDAGITMGLFSYPVLMAADILMFNAHKVPVGRDQIQ
                     HVEMARDIGQRFNHLFGNGRELFTLPEAVIEEDVATLPGLDGRKMSKSYDNTIPLFGS
                     AKQLKDTVARVVTDSKLPGEPKDPDNSHLFTLYQAFAAPTQQADFRAALEGGMAWGEA
                     KQALCNLLEAELGEARERYHALISKPADLEDILLAGATKARKTATPFLGELREAVGLR
                     SFRSQAVNTAGEKKKVGKSARFVSFRDDDGSFRFRLLDAAGEQLLLSKSFVDGKAAGM
                     VNKRLQSGEALDVREQDGGFGVWLDDECVAQSPVFADSSACQAAIQRLREALAPAE"
     gene            38340..39433
                     /gene="zapE"
                     /locus_tag="VFF22_19715"
                     /pseudo
     CDS             38340..39433
                     /gene="zapE"
                     /locus_tag="VFF22_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253128.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
     gene            39575..40912
                     /locus_tag="VFF22_19720"
     CDS             39575..40912
                     /locus_tag="VFF22_19720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZX19083.1"
                     /translation="MTFASLGLIEPLLRTLDSLDYKTPTAVQAQAIVPVLQGRDLMAA
                     AQTGTGKTAGFALPVLQRLMMEGPEVSSNSVRALVLVPTRELAEQVLQSFITYGQHLP
                     LRSYAVYGGVSINPQMMKLRKGVDVLVATPGRLLDLFRQNAVKFNQVQTLVLDEADRM
                     LDLGFANELESVFRALPKKRQTLLFSATFSEAIRSMAGEMLNDPLSIEVSPRNAAAKS
                     VKQWLIPVDKKRKSELFLHLYQAKRWSQALVFVKTRKGVDELEAEMQRHGISADSIHG
                     DKPQATRQRALQRFKDGEVKVLVATDVAARGLDIDDMPLVVNFDLPIVAEDYVHRIGR
                     TGRAGSTGQAVSLVCADEVQLLSAIETLTQQVLQRVDEPDFIPDHRVPQTLPGGQVVK
                     KPKKAKKPKVVGGGKGGSLGRWMEADEPAAPTVKAVRKVPSFGGKPGKPSRKP"
     gene            complement(40959..41336)
                     /locus_tag="VFF22_19725"
     CDS             complement(40959..41336)
                     /locus_tag="VFF22_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZX19084.1"
                     /translation="MLQSVELKTFVPARDFALSQAFYQALGFKPGWVGDDLAYFSHGD
                     HCAFLLQDFYVKEQAENFVMHLLVEDVDAWWQKVLDEQLSERFEVRLVPPQDQPWGMR
                     DFVIFDPSGVLWRIAQNTQRVPD"
     gene            complement(41417..41872)
                     /locus_tag="VFF22_19730"
     CDS             complement(41417..41872)
                     /locus_tag="VFF22_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZX19085.1"
                     /translation="MPAQIDIQEVHWLLDIVQCIDVGVIVLDRQYQVEVWNSFMENHS
                     GMGPDEVHGKTLFSLFPEINETWLRRKVDTVVQLGTRAFSLWEQHPYLMHFKNYQPIT
                     GQEDFMYQNVTLLPLAGAKGDVEHICVVIYDMTAAATHKKQLEAAQASR"
     gene            complement(41876..42877)
                     /locus_tag="VFF22_19735"
     CDS             complement(41876..42877)
                     /locus_tag="VFF22_19735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZX19086.1"
                     /translation="MIPLVVCDDSNMARKQLIRALPPEWPVTISQATNGQEAIAALRQ
                     GLGQVMLLDLTMPVMDGYETLAALRAEGLACKVVVVSGDVQDEALRRVKELGALAFIK
                     KPADPEVLRATLLELGLFDPKATPGAIAQAAVLDDLKVNFRDALREVTNVAMGRAAAL
                     LAKALGVFVQLPVPQVNIFEVSELHMTLTDAMRGERLSAVCQGFIGDGIAGESLLLFH
                     DSEVADMARLLDWQPKDDLETSEMLLDLASILIGACLAGIAEQIDVRFSQGHPQLLGQ
                     HSSIERLIHLNQRRWKKTLAVEISYSLEAHNIHFDLLLLFTEDSIKRLTNKINYLMS"
     gene            complement(42964..44208)
                     /locus_tag="VFF22_19740"
     CDS             complement(42964..44208)
                     /locus_tag="VFF22_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007946620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HZX19087.1"
                     /translation="MNSALPKPAPRVAIIGGGPAGLIAAEVLAQGGVQVDLYDGMPSV
                     GRKFLLAGVGGMNITHSEAKPAFVARYGQRQSEVAELLQDFDADALRAWIHGLGIDTF
                     VGTSGRVFPADMKAAPLLRAWLKRLRELGVQIHTRHRWLGWTNDGALRIASSTGEHTV
                     NTDATLLALGGGSWARLGSDGAWVSLLQDRGVTISPLQASNCGFDVSAWSPFLRDKFA
                     GAPLKNVSLNLAGAAPRQGECVLTASGIEGSLVYALSADIRETINQHGNATVHLDLLP
                     QTPLAKLQAALSKPRGSQSMAKHLHRQAGIDGVKATLLRELASAAAFNDPALLAGVIK
                     SLPITLIRPRPLDEAISTAGGVPFEQLDAQLMLKPLPGTFCAGEMLDWEAPTGGYLLT
                     ACFASGRRAAQGILEWLQHPAE"
     gene            44321..45454
                     /locus_tag="VFF22_19745"
     CDS             44321..45454
                     /locus_tag="VFF22_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZX19088.1"
                     /translation="MSDLYPSIDPDGLIEYSVVYTDRSLNHMSQSFQGVMKDISSTLK
                     QVYNAHAMAIVPGSGTYGMEAVARQLATGQKCLVLRNGWFSYRWTQIFEMGQIPSESI
                     VLKARPMAEGSQAAFSPPRLVDVLAVIAAEKPQVVFAPHVETSSGMILPDGYLRAVAD
                     AVHAVGGLFVLDCIASGTLWVDMQACGIDVLISAPQKGWSASPCCALVMLSEAAQARV
                     EATQSTSFACDLKKWLQIMQAYEQGGHAYHATMPSDALARFRDAMREAEAIGFATVRN
                     QQQELGDRVRALLASKGFKSVAAEGFEAPGVVVCYTTDASIKNGSKFAAQGLQIAAGV
                     PLQCDEPADFQTFRLGLFGLDKLGNIDRTVATLEQALDKVLAG"
     gene            complement(45583..46746)
                     /gene="ampC"
                     /locus_tag="VFF22_19750"
     CDS             complement(45583..46746)
                     /gene="ampC"
                     /locus_tag="VFF22_19750"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class C beta-lactamase"
                     /protein_id="HZX19089.1"
                     /translation="MHGRLKTLLTALALATCTVAQATDRLQSLVDATVRPVMEEYDIA
                     GMAVAITHNGQAHYFNYGVARLDDQQAVTSDTLFEIGSLSKPLTATLAALAQTTGKLT
                     LADSAGQHWPELRGSQLEHASLLNLGTYTAGGLPLQFPDRVTDPASMLAYYRQWQPEH
                     IAGTHRRYSNPSIGLLGMLAAKSMGQPFPTLMEGTLFPALGMPHSYIQVPAAQMPRYA
                     YGYSKDNQPLRVTPGMLDAEAYGVKTNAKDMLRFVQANLNSAGLSEDLQRAIAITHTG
                     YYRVGEMTQGLGWERYPYPISLERLQAGNSPAMALESQPAKRLAPPETEPGDSLFNKT
                     GSTGGFGAYAVFVPSQNLGLVMLANKNYPNTARIKAAHAIVTALDKATNASEQ"
     gene            complement(47002..47922)
                     /locus_tag="VFF22_19755"
     CDS             complement(47002..47922)
                     /locus_tag="VFF22_19755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HZX19090.1"
                     /translation="MRLPLVYHDDYSPPFPANHRFPMEKFRLLRDHIVASGLSTDAEL
                     LRPELCPTDVLALTHCPAYIQRFASGELPHEEQRRLGLPWSSALAQRTIRAVGGSLLA
                     AELALQHGLACHLAGGTHHAHYDHPAGFCIFNDLAVISQYLLASGKAQRVLIFDCDVH
                     QGDGTARLLENTPEAITVSLHCEKNYPARKAASDWDIPLPNGMGDAEYLKVVDDCLNY
                     LLALYQPDIVLYDAGVDVHQHDALGYLNLTDAGIAARDAAVLNHCLDRDIPVVGLIGG
                     GYDKDRMALARRHGILHHNAAKVWQAHGLG"
     gene            48107..48976
                     /gene="tesB"
                     /locus_tag="VFF22_19760"
     CDS             48107..48976
                     /gene="tesB"
                     /locus_tag="VFF22_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HZX19091.1"
                     /translation="MSQVLNELVALLTLETIEENLFRGVSQDLGFRQLYGGQVLGQCV
                     SAASQTVDTARHVHSMHGYFLRPGDATLPVVYQVERVRDGGSFSTRRVTAIQKGKPIF
                     TCSASFQSDEEGFHHQNLMPEVPGPEELKSETELARLVVDALPERMRERAVSDKPIEI
                     RPVTVGNPFAPKPSEPVKHVWFRAAGELPDDPQLHKYLLGYASDFNLLTTSMLPHGVS
                     VWQKFMQVASLDHSLWFHGNLRMDDWLLYSMDSPWAGNARGFSRGSIFNRQGQLVASV
                     AQEGLTRVREDWK"
     gene            48973..49560
                     /locus_tag="VFF22_19765"
     CDS             48973..49560
                     /locus_tag="VFF22_19765"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZX19092.1"
                     /translation="MSLSAARHWVFDMDGTLTLAVHDFEAIKRALDIPLEDDILGHLA
                     ALPTAVAAAKHAWLLEHERELALTAAPAPGAIELVRALHGRGCRLGILTRNAHELALL
                     TLRAIGLDDCFAVPDVIGRDEAPPKPDPGGLLHFARTWQVAPSELVMVGDYRFDLECA
                     RAAGARSVLVNLAENPWPELADHFAVDCRALQRSL"
     gene            complement(49611..50030)
                     /gene="cueR"
                     /locus_tag="VFF22_19770"
     CDS             complement(49611..50030)
                     /gene="cueR"
                     /locus_tag="VFF22_19770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HZX19093.1"
                     /translation="MNIGQAAKKTGLSAKMIRYYESIDLLPAAGRTDSGYRQYSQQDL
                     HRLAFIKRSRDLGFSLAEVSQLLALWQDRQRASADVKALAAAHISELERKIEELSGLR
                     DTLRDLMDNCQGNDRPDCPILKDLESGCCNHNAPITP"
     gene            complement(50155..52554)
                     /locus_tag="VFF22_19775"
     CDS             complement(50155..52554)
                     /locus_tag="VFF22_19775"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZX19094.1"
                     /translation="MSSLTTFNLPISGMTCTSCAGRIERALAKVTDVASVSINLASEQ
                     ARVEAPADSLEQLVNAVSAAGYEVPSERLELALSGMTCASCAGRIEHALTQQPSVLSV
                     SVNLASERAQLQVLRGTDANALLQAVSQAGYRASVLDDATQAQPTAERHLQRERWALL
                     LAIVLTVPLVIPMLVEPFGLHWMLPAWLQFALATPVQFVFGARFYRAAWNALRARAGN
                     MDQLVAIGTSAAYGLSLYQWAMSPAGSMPHLYFEASAVIITLILLGKYLESRAKRQTS
                     SAIRALQALRPERALRLQDGVEQWVALGELQLGDTIVVKPGERFPVDGLVREGQSHAD
                     EALISGESLPQPKQPGDSVTAGAINGEGRLLVETTALGAETVLSRIIRLVEDAQAAKA
                     PIQKLVDKVSQVFVPSVLLIALATLLGWLLLGAGIETALLNAVAVLVIACPCALGLAT
                     PTAIMAGTGVAARHGILIKDAEALEVAHSVTLVAFDKTGTLTSGAPRIAHLRAVDGPD
                     TEHSTQLLQLAGALQRGSEHPLAKAVLDQCTEQQLSLAEVSNSKALAGRGIAGQIGQR
                     QLALGNKRLLEEHFLHHDALATDALAWEAEGRTLSWLLELAPQPQVLGLFAFGDTLKP
                     GAAEAIAALTAQGIASHLISGDNPGSVKAVAAALGISAAHAEVLPADKAAIISELKKN
                     AVVAMVGDGINDAPALAAADVGIAMGSGTDVAMHAAGITLMRGDPRLVADALDISRRT
                     YNKIRQNLFWAFAYNLVGIPLAAAGLLNPMLAGAAMALSSVSVVSNALLLKTWKPKDR
                     S"
     gene            52704..52916
                     /locus_tag="VFF22_19780"
     CDS             52704..52916
                     /locus_tag="VFF22_19780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HZX19095.1"
                     /translation="MQVSQVHAFRVDGMTCQNCVKTITEALLACDPAADVKVKFPGGE
                     VRVSSSVPVDQLMVAIVEQGYGVSLA"
     gene            complement(52903..53547)
                     /locus_tag="VFF22_19785"
     CDS             complement(52903..53547)
                     /locus_tag="VFF22_19785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZX19096.1"
                     /translation="MRRTKEEAEKTRVAILEAAETLFLEKGVAHTSLEHIARHAGVTR
                     GAVYWHFANKADLFSAMLGQVRLPPEQLAERMRGCPAHDPLMTLRELCIEAIENLARS
                     PQKKRIFTILLRRCEFTEELREAEERHEAFINLFIALCEQQFSAPAVAPRLYPGLSPR
                     LAARIVHALLIGLFHDWSRDPNLFDPLSDTAPMIDACFRGLIRDWPDAAVLTPG"
     gene            53686..54825
                     /locus_tag="VFF22_19790"
     CDS             53686..54825
                     /locus_tag="VFF22_19790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZX19097.1"
                     /translation="MLFSRRLPVTAGSLLLAFLAAPLFAADAPPAPEVVVETVKAEAL
                     PLELEYSARTAGFREVQVRAQVSGILQERTYLEGSQVKQGQVMFRIDPRTYQAALSRA
                     KGALAQEQARYRQTERDLKRIRELQKKGFASESELDNAISNFEQSKANIQAAEAEVQS
                     KQIDLDYTTVKAPISGITSKETVSEGSLMVAGDPSASLLSNITQLDPIYVNFAAPDSD
                     VESVRSGLQNGSLVLPEDGKMSVQIKLGDGSVYPLEGKVDFTDSLVDRGTGTVSARAV
                     VPNPDQTLLPGQFVRVQVKGLSIPNAMTLPERAIAQGPGGTFVYVVDEGGVARMRQVT
                     TGHTAKGRWVIVSGISAGDRVIVEGLPKVRPDSPVKVVAPAASQS"
     gene            54838..57966
                     /locus_tag="VFF22_19795"
     CDS             54838..57966
                     /locus_tag="VFF22_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZX19098.1"
                     /translation="MISRFFIDRPVFAAVISIIIVLAGLMAMRSLPIAQYPQILPPQV
                     SVSASYAGASAQVIAETVAAPLEQSINGVEGMIYQQSNSGGNAMSLSVYFEVGRDPDQ
                     ATIDVNNRVQAALAKLPEEVRRQGVKVQKKSSDILQVVTLYSPDGSRDPVFISNYALI
                     NVIDELKRLPGVGDVSQFGSKDYSMRIWLRPDKLAQYNLTPTDVVNSIREQNSQFAAG
                     SFGQQPLKQEQDFTYTVTTQGRFTDPKEFENVILRTDDTGASLLLKDVARIELGAQDY
                     SLMTSLNGQQNAAFGVYLQPGANALDTAEAVSNTLARLSKNFPSGMTYKVPYDTTKFV
                     QVSIKEVIHTFFEALILVVLVVFIFLQNWRATLIPVLAIPVSLVGTFAGMYMLGFSIN
                     LLTLFGMVLAIGIVVDDAIVVIENVERVMATDKIGPREATIKAMEEVTGPIIAIVLVL
                     CAVFVPVGFLGGLAGEMYKQFAITIAVSVVISGIVALTLSPALCALLLKPGHHEPAAP
                     FRAFNRVFDKLTSGYTAGVRFFLKRSAVGLLLFGAMIALMVVLFNRVPSSLVPNEDQG
                     YVINAYFLPPAASLTRTEKLTGDVTQQLMAHPAVQDVVTFAGFDVLTFGTRSNAGVSF
                     VPLKDWSERTTPELDARNLTGEFMGMGAAQKDGVVLSFNPPPITGMSTTGGFEGFIQD
                     RSGGTTAQLAEKVQAFLAAAAKRPELAGVQSTFSANVPQYFIDLDRTKARALGVAVND
                     VFTAMQATFGSYYVNDFSLYGRTFQVSLQAEAEFRSKPEDLSQVYVRAGSGELVSLAS
                     LVKVERILGPDTYARFNVYPAAKILGGPAPGFSSGQALSAIQEVADEVLGSDYSIGWT
                     GSAFQEVASQGSGSSAFIFGLIMVFLILAAQYERWTLPLAVVTAVPFAVFGAILAVWL
                     RGIENDLYFQVGLVTLIGLAAKNAILIVEFAVLCRRQGMGIFESALEASRLRFRPIIM
                     TSLAFILGVVPLAISSGAGSASRHSIGTGVIGGMMAATFLAIFLIPMFYLLVESLAEK
                     IGKGRKKADTPA"
     gene            58056..59240
                     /locus_tag="VFF22_19800"
     CDS             58056..59240
                     /locus_tag="VFF22_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HZX19099.1"
                     /translation="MPLRLLLILGALSAFGPLAIDFYLPSFPTLARAFSTDVEHVQLS
                     LAAYFAGLAIGQLAYGPLADRFGRRKPLLVGVMLFSLASLACALAPSLEWLIVARFVQ
                     ALGGCAGMVVSRAVVRDLCDPISSAKVFSQLMLVMGLAPILAPLGGGLLLSTLGWPSI
                     FICLTLFSFICLLAVAKWLPETLAKDAPPAPLRGALGEYKRLFADLPFLGYALTGGFA
                     IAGMFAYIAGSPFVFIELYGIPAEHYGWLFGSNALGFILVAQLNAWLVARHGPAYWLG
                     KTVWFYLACGLALLLVALAKPQALWPLLVPLFGCIASLGILLPNASACAMAGQGSHAG
                     SASALFGSVQFVIAASAAALVGMLHDGSAWPIAVVIFACGVLAVGFSLFTRWVERGAI
                     RA"
     gene            complement(59283..59462)
                     /locus_tag="VFF22_19805"
     CDS             complement(59283..59462)
                     /locus_tag="VFF22_19805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008368808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX19100.1"
                     /translation="MNVMIQPLKTAAGAGWQVIFGKQSVTFRSESEARQFVSTLQARL
                     SAPHRLPFEQQRAAS"
     gene            complement(59532..61415)
                     /locus_tag="VFF22_19810"
     CDS             complement(59532..61415)
                     /locus_tag="VFF22_19810"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZX19101.1"
                     /translation="MRPDLQGPLRLAGVYILFSALWIFGSDYLLHALVKDNELVAELQ
                     TGKGLLFILLSTGLIFLFSHRDRQAQRHLLEKLTHNSRLLQHTQRNAGLGSWEYLNGF
                     HWSEEALRLLDRAPDSQQCSTEQLLSWLHPAERSAVQRALLALLEGRSAMMVSARLQQ
                     RQPHQDTWLMLRGEVDEHGRILGTVQNISAQKRDESALRESEQRFRLLFEQTPRIAVQ
                     GYDRERRVIYWNQASALLYGYAVHEVMGRRLEELIVPLQARSKVASAIKLWQLGGPPI
                     PAGEVQLQRKDGSLVWVYSSHLMMRNSLDQMELYCVDIDLTEQKKMDSELQISESRYR
                     SLVEHLGDAVFITDANDKLSFLNPAWEQISGYPIHESLGNPLQRFIPELGQTMLRQGL
                     EDLRSGKLDSLRLECQLLTHSGQARRVELHLNLSLPELSLHGSLHDVHERHQAQHLQQ
                     ARNAVLDELLGLQPLHSILNGISSSLESLQPQMRVSIMLLDEHQRLHLGAAPSLPEAY
                     CQAIEGIGAALEVGSCGHAAATGELVIAEDLSQHPYWRDYQSVALAAGLHACWSLPFK
                     DDSCQVLGTFGIYYPQPSRPSAADIALVTEFTRLAGLAVQFRAAPSTPNVGESDQRYV
                     SRD"
     gene            complement(61576..62592)
                     /locus_tag="VFF22_19815"
     CDS             complement(61576..62592)
                     /locus_tag="VFF22_19815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HZX19102.1"
                     /translation="MKAVAYYQPLPTDHADALQDVELPAPTPGPRDLLVEVKAISVNP
                     VDTKIRRNVAPEDGAAKVLGWDASGIVKAVGSEVSLFQPGDRVYYAGAINRAGANSEL
                     HVVDERIVGRMPAKLGFAEAAALPLTAITAWELLFERLQITRGNADLGQSLLIVGAAG
                     GVGSILLQLARQLTGLTVIATASRPETQAWVSELGAHHVIDHSLPLSEELKRIGIHQV
                     THVASLTQTDKHFAQLVEALAPQGRLALIDDPEQPLDIMQLKRKSLSLHWELMFTRSL
                     FETADMIEQHHLLDRVAELVDAGTLRTTLGEHFGTINAANLRRAHTLLESGTAKGKIV
                     LEGF"
     gene            complement(62603..62896)
                     /locus_tag="VFF22_19820"
     CDS             complement(62603..62896)
                     /locus_tag="VFF22_19820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HZX19103.1"
                     /translation="MTTPLTLIATITARPGHAEAIEAGLRQLVPPSQAEAGCLQYNLH
                     LHEEQPNRFIMIEQWQDAAALSAHQQTAHFLHFGETCGSLLEQVDHQLMHRIL"
     gene            63001..63918
                     /locus_tag="VFF22_19825"
     CDS             63001..63918
                     /locus_tag="VFF22_19825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX19104.1"
                     /translation="MLRFDDLQLFVTTADLGNLSAAARRLDISPAVASAGLKRLEQQL
                     QVRLFSRSTRSLRLTPEGELFLGHARLALQSLDDGRQQLAGSKVGISGPLQLSAPSDF
                     GRNTLLPWLDEFQQAHPQVQLRLLLGDRVADLFREPVDIALRYGAPEDSSLVALPVAA
                     LNRRVLCAAPQYLHKHGVPQSVEALAGHNCLLYMLGGRLHDRWRFSDGKREQGISVKG
                     DRVSDDADVVRRWAVSGAGLVYKSWLDVAGDVRAGRLQVLLPELRGEPTPLNLICAHR
                     AQLSKPVLLLREFVQLRCAELLADAPWPN"
     gene            64001..64387
                     /locus_tag="VFF22_19830"
     CDS             64001..64387
                     /locus_tag="VFF22_19830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 domain-containing protein"
                     /protein_id="HZX19105.1"
                     /translation="MKMTRTDRRLLAWMLYFSVLFSAFACSIGHGQMAGLQLSGLGSQ
                     YCSFEGNFGAGADLEGSGVVAPNPATGSGCSLSSTFSAIILAAFFGLLGLLAPSRAPL
                     LTFFFIPRPVRYLWPSANPRASPSTP"
     gene            64478..65848
                     /locus_tag="VFF22_19835"
     CDS             64478..65848
                     /locus_tag="VFF22_19835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HZX19106.1"
                     /translation="MSKTAPSFYNLAWRWHFYAGLFVIPFLILLSVTGIIYLFKPQLD
                     QLMYADLLQVTPAEQALSADQQLALVRQAYPRASISQYLPPVNAKRSAQFVLEADGQG
                     LNVFVDPYSARILGSQDAQLNLQAIARQLHGDLMVGTLGDRLIELAAGWGIVLVVSGL
                     YLWWPRGRSGAGVLWPRLSARGRLLWRDLHAVTGFWGSLLLLFMLLTGMTWTGFWGAQ
                     FAGAWNHFPVAMWDDVPQSGKLTGSLNTSSQQTVAWAVETTPLPASDPHAAHNGHATA
                     EAPAAPANLLQYVVDTAAERGVQPGYSITLPKGETGVYSIAMFAADPRNDATLHIDQY
                     SGAVLADVRWKDYGLVAKTVETGVMLHEGKLFGLANQLLMLAVCLLVLLSAISGLIMW
                     WQRRPAGKFGVPPLRHDLPRWKTAIVIMGLLGAAFPLVGASLLLIWALDWLLLSRLPG
                     QRLANG"
     gene            65937..66419
                     /locus_tag="VFF22_19840"
     CDS             65937..66419
                     /locus_tag="VFF22_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HZX19107.1"
                     /translation="MLLKKTLLIAALLSPSLFASAHEYTAGELHIAHPWSREMPAVAP
                     TAAAYFVVHNKGAEADRLLSVSTPVAGKAELHEHIHADGLMKMQQVQNVAIPAGGEVK
                     FEPMGYHVMLFNLKQHAKDGERFPLTLTFEKAGAVEVEIAVHKDAPAGEKTHGEGHAH
                     "
     gene            66551..66922
                     /locus_tag="VFF22_19845"
     CDS             66551..66922
                     /locus_tag="VFF22_19845"
                     /inference="COORDINATES: protein motif:HMM:NF022610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 domain-containing protein"
                     /protein_id="HZX19108.1"
                     /translation="MPPDRSFTAHYRASWMALLAMLLLNLGPLGSQLSQPAEPAWLTE
                     LACGEHAGGHNSPLDHDVLWAKCGYCTLLLNSPATSCASPTLALAAVVPADRHIAPVR
                     SGVVRSAIFPGSRSRAPPAYS"
     gene            67004..69091
                     /locus_tag="VFF22_19850"
     CDS             67004..69091
                     /locus_tag="VFF22_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent copper receptor"
                     /protein_id="HZX19109.1"
                     /translation="MSTRSLLISAALFRLAPLCAGLLLAGQGHAAQTAEHIAELQLSP
                     LVITAVQQRSPLTVVAYPKDPRQPVPASDAADYLKTIPGFSAIRSGGSNSDPVLRGMF
                     GSRLLLLTDGGQMLGACPGRMDAPSSYISPETFDLLTVTKGPQTVIWGPGASAGVVRF
                     DRAPESFGELGARLHASVLAGSNGRFDQTLDGAVGGERGYLRVTGNRSHSDDYDNGNG
                     DTIPSRWDKWNSDVALGWTPDADTLIELTAGRGDGEARYGGRGMDGTQFKRESLGLRV
                     ERSNLGGVLDKVEAKVYYNYADHIMDNFRLRMPDPTSMMRNPMESQVDRRTLGARLAA
                     TWKWDDLELVTGLDAQRSEHRARRSTYRMMGGVFTGADRYPWKKDALMHNYGLFAEST
                     WQMADRDRLISGARLDRASAKDLRPSFSSMMGMPTPNPTADETRADTLPSGFVRYEHD
                     LSGSPTTVYAGIGHVQRFPDYWELFSAGLNGPAGADNAFDGIKPEKTTQLDIGIQYKG
                     EALQAWASAYAGQVRDYILFDYRSMSTQADNIDARIMGGELGVAYDLSANWKTDATLA
                     YAWGKNSSDGEALPQMPPLEARLGLTYQRDDWSVGGLWRVVDGQGRTADGRGNVVGQD
                     FGDSAGFGVLSLNGAYRVSKQVKLSAGVDNLLDKNYAEHLNLGGDAGFGFPADTRIDE
                     PGRTLWAKVDFDF"
     gene            69229..69495
                     /locus_tag="VFF22_19855"
     CDS             69229..69495
                     /locus_tag="VFF22_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010176882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HZX19110.1"
                     /translation="MRKLLLLSLVLTSPLTFAATQCTTAEKAQWQDQDKFQADLKAQG
                     YEIKKFKVTGGNCYEIYGFNKDGQKVEIYFDPVSGKVIKGGVEG"
     gene            69495..70028
                     /locus_tag="VFF22_19860"
     CDS             69495..70028
                     /locus_tag="VFF22_19860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HZX19111.1"
                     /translation="MNTVTTRLWDPLVRLFHWSLASAFLANYFFTEEGENWHRWFGYY
                     AVAWLAIRLVWGFIGTPAARWADFWPTRARLAEHLRALLSGRDYHRMGHSPLGGLVMI
                     GMMLLMLGLGVTGFLMEEVDYFWGADLPEDIHEFCANALMALVGLHIAAALFESYRLK
                     ENLPLSMITGKRRKLDH"
     gene            complement(70107..71054)
                     /locus_tag="VFF22_19865"
     CDS             complement(70107..71054)
                     /locus_tag="VFF22_19865"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016715423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HZX19112.1"
                     /translation="MYDWLNALPKAELHLHLEGSLEPELLFALAERNKIALPWNNVEA
                     LRSAYAFNNLQEFLDLYYAGADVLRTEQDFYDLTWAYLLKCKAQNVIHTEPFFDPQTH
                     TDRGIPFEVVLRGIKQALVDGEKQLGISHGLILSFLRHLSEEEAFKTLEQAMPFRDAF
                     IAVGLDSSEMGHPPRKFQRAFDKARSEGLLTVAHAGEEGPPEYIWEALDLLKIRRIDH
                     GVRAIEDERLMQRIIDEQIPLTVCPLSNTKLCVFDDMRQHNILQMLERGVKVTVNSDD
                     PAYFGGYVTENFAALHDSLGMTQEQAKRLAQNSLDARLV"
     gene            complement(71255..72220)
                     /locus_tag="VFF22_19870"
     CDS             complement(71255..72220)
                     /locus_tag="VFF22_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016703806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HZX19113.1"
                     /translation="MSTLPIIDIAPLYSDDQAAWPAVAEQIDRACRDWGFFYITGHPI
                     SAARIAELLSAAKTFFALPAAEKLKIDITQTAHHRGYGAIATEQLDPSKPSDLKETFD
                     MGFHMDLNHPEVLAGKPLRGTNRHPDLPGWATLMEQHYADMQALAQTLLRAMTIALDI
                     NRDFFDTRFHEPISVLRMIHYPPRHTASSAEQQGAGEHTDYGCITLLYQDDAGGLQVR
                     HRDGQWIDAPPIAGSFVVNIGDMLARWSNDRYTSTPHRVISPLGVDRYSMPFFAEPHP
                     DTEISCLPNCSDAANPPKYPSVTSAEYLMSRFADTYAYRREEQAS"
     gene            complement(72351..73433)
                     /locus_tag="VFF22_19875"
     CDS             complement(72351..73433)
                     /locus_tag="VFF22_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003197490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HZX19114.1"
                     /translation="MFEKSKKPLLRTLVAALGFSATLGASAADPLKVGFVYIGPIGDH
                     GWTYQHEQGRQAVIKQLGDKVETSYVENVPEGADAERVIRNMAKGGYDLIFTTSFGYM
                     NPTLKVAKQFPKLTFEHATGYKQDKNLGTYLSRSYEGRYVGGFLAAKMTKTKKIGYVA
                     SFPIPEVIRDINAIQLALDKYNPGTEIKVVWVNSWFDPGKESDAANALIDQGVDVVFQ
                     HTDSPAPIQTAERRGVYSVGYASDMAHFGPKAVLTSIVNDWGPHYVKSTQAVIDGKWA
                     PQDFWGGLAEDTIRLPISDLVPAEVKTEAEQIIADIKSGAFHPFTGPIKDQSGVEKIP
                     AGVAATNAELASMNYYVENVNAELPK"
     gene            73777..74226
                     /locus_tag="VFF22_19880"
     CDS             73777..74226
                     /locus_tag="VFF22_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010166218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZX19115.1"
                     /translation="MPHSKQLRIGRFSEDQRIYLLTATVETREPIFADFLLGRLLAAE
                     LKAAHFDGLVESLAWVVMPDHLHWLLVLHRGSLSELMRRVKGRSARQINPLAGRRGQL
                     WQDGFHDHALRHEEDVLPVARYIVANPLRAGLVERVGDYPLWDAVWL"
     gene            complement(74329..75255)
                     /locus_tag="VFF22_19885"
     CDS             complement(74329..75255)
                     /locus_tag="VFF22_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX19116.1"
                     /translation="MDLDLLSNIFYAMVRTGTPLLLVALGELICEKSGVLNLGQEGMM
                     LFGAVIGFIVALSTGNLWLGVLLAMAAGMLLSMLFAAVALGFNANQVATGLALTIFGV
                     GLSTFVGAAWVGKPLAGFEPIAIPLLSEIPLIGRMLFAQDILVYLSFALFALVAWVLL
                     KSRIGLIIQAVGENPDAASAMGLPVLRVRTLAVLFGGAMAGLAGGYLSLAYTPMWAEN
                     MTAGRGWIALALVVFASWRVLRVLLGAYLFGLASIIHLVAQGIGLSIPSNLLAMLPYV
                     ATILVLVLLSRDAIKTRLFAPVSLGKPWQPGH"
     gene            complement(75425..76522)
                     /locus_tag="VFF22_19890"
     CDS             complement(75425..76522)
                     /locus_tag="VFF22_19890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX19117.1"
                     /translation="MLLSLEPRGQQSRAMLWLSPLLAAVLTLCSGALLFTLLGHDPLQ
                     TLHTLLIAPLADWYGVSELLVKALPILLCALGLAVAYQARIWNIGAEGQLLLGALAGS
                     AMAIQIIDWDSRWALVWVVLAGTAAGAAWGGLTAWLRTQFNANEILTSIMLNYIALNL
                     LLFFVHGPLKDPAGFNFPESAMFGDASRLPLVSEDGRLHAGLYFALLALVAVWVLLHK
                     SFLGFQIKVLGLDRRAAGFVGFREKRLVWFALLVSGGLAGLAGVGEVAGPIGQLVPQV
                     SPGYGYAAITVAFLGRLNPFGILFASLLMALLYLGGENAQMTMNLPQAITQLFQGMML
                     FFLLACDVLILYRPRLKWKWASLPVLKKAAA"
     gene            complement(76534..78105)
                     /locus_tag="VFF22_19895"
     CDS             complement(76534..78105)
                     /locus_tag="VFF22_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX19118.1"
                     /translation="MPMSSSPSVPRLQLSGISKRYPGCLANDRIDLSIAPGEIHALLG
                     ENGAGKSTLMKIIYGVTQPDSGEIRWQGEPLKMRDPAMARSLGVGMVFQHFSLFETLT
                     VAENIALAMGATAGTPKQLEPKIREVSQRYGMALEPGRLVHSLSIGERQRVEIVRCLM
                     QDIKLLILDEPTSVLTPQEADELFVTLRRLAAEGCSILFISHKLGEVRALCQSATVLR
                     GGRVSGHCVPAECSDLQLARLMVGDAEGLESSYPKVIGGLPRLRVDDLSWHNADPFGC
                     HLSQIRLEVRSGEIVGIAGVAGNGQDELLALLSGETRLPRSERERISLDTQPIADLYP
                     DARRKLGLAFVPAERLGHGAVPDMSLADNAMLTAFQQGLLSKGLIQRGKVRALADEII
                     KRFAVKTPDADAAARSLSGGNLQKFILGREILQNPKLLVAAHPTWGVDVGAAAAIHRA
                     LIALRDAGAAILVISEDLDELFQISDRIGALCSGKLSPLAATAETEAVDVGRWMAGEF
                     APITSTQAAPAANMS"
     gene            complement(78375..78833)
                     /locus_tag="VFF22_19900"
     CDS             complement(78375..78833)
                     /locus_tag="VFF22_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007977278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZX19119.1"
                     /translation="MPVKPHGRNLRRGRYSEPDRIYLLTTTLLQRRPLFNDYTLARLL
                     VKELRSAHEQQQVASLAWVVMPDHLHWLVQMQTGSLDKLMRRIKSNSARQINQYLGTQ
                     GPIWQPGYHDRALRQDEDLQSAARYIVANPLRAGLVQRIGDYPLWDAVWL"
     gene            complement(79022..79606)
                     /locus_tag="VFF22_19905"
     CDS             complement(79022..79606)
                     /locus_tag="VFF22_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HZX19120.1"
                     /translation="MPFTLLGPCEYREEIRKSRFIALAAPVTSAAEAQAFIAAHSDAS
                     ASHNCWAWKVGQQYRFSDDGEPGGTAGRPILAAIETQEMDQVAVLVIRFYGGIQLGTG
                     GLARAYGGSANKCLQSAPRIELVAREQYHCHCLFAELPLLKARLTELDCLLTSEDYDA
                     TGAELHLAIPAPRVAELQRLLADISRGRSELRPQ"
     gene            complement(79620..80291)
                     /locus_tag="VFF22_19910"
     CDS             complement(79620..80291)
                     /locus_tag="VFF22_19910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZX19121.1"
                     /translation="MTVEVLAHRADPNNKPAGRIRQKNEEAIIKAAEEEFARHGFKGT
                     SMNTIAQNVGLPKANLHYYFSNKLGLYQAVLINILELWDNTFNNLSVDDDPATALAAY
                     IRAKMEFSRRHPKASRIYAMEVISGGETLSQMLSQDYQTWFRGRAAVFEAWSEAGKMD
                     PVDPVHLIFLLWGSTQHYADFSSQICRVTGTSRLTKQDYEVAGDQLIRIILKGCGLTP
                     PAPDV"
     gene            complement(80533..81834)
                     /locus_tag="VFF22_19915"
     CDS             complement(80533..81834)
                     /locus_tag="VFF22_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX19122.1"
                     /translation="MSPAQRLAQAINARPAELLAVLCGFGLFFCLFSGYFMLRPIREA
                     MGIRGGVENLQWLFTATFLVMLTAVPLFAWLNSTVARARYIDWVYGFFILNLLAFAAL
                     FQHSGDDIWVARVFYVWISVYNLFVVSVAWSLMADVFDSAQARRLFAFIAAGASCGGL
                     AGPALGALLIERFGQSGLILLAAVLLGMAILAKQQLMAWRAIGGAGRPGAPVSESPHR
                     PVIGNPFSGLTRVLASPYLLGISAFVILLATASTFLYFEQARLVAETFTERNEQIRVF
                     GLIDFSVQALSLLAQLFITGRIAQRFGIRALLAGVPLLACLGFIGLALAPTFAMLAAL
                     MIVRRVGEYAFVRPGREMLFAPLDAESKYKAKNFIDTVVYRAGDAVSAWAKALLDMLA
                     NGVLIAAIAGALCAAAWGALGWYLGGRREQAAPALLEQNHS"
     gene            complement(81871..82788)
                     /locus_tag="VFF22_19920"
     CDS             complement(81871..82788)
                     /locus_tag="VFF22_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZX19123.1"
                     /translation="MPSRRHFIQGSASLLALAATPWLPSLAYADPSQLLKRAIPSNGE
                     MLPVIGLGTSRTFDVGLTNDSLKPLDSVMRTFINGGARLIDTAPSYGASEAVTGELLK
                     RTNTQGKAFLATKISATGRERGMAQIEASLQALQTDTLDLVQVHNLQDTRTQLALLRE
                     LKAEGKVRYIGITHYIESAHDDLLEVLENESVDFVQLNYSVGERNAEKRLLPYCADHG
                     IATLINRPFQRAQLLSRVKGKSLPSWAMETDATSWAQLLLKFILANPAVTVVIPATSN
                     PRYMADNLKAGIGRLPDAALREKIVQAFS"
     gene            complement(82919..83836)
                     /locus_tag="VFF22_19925"
     CDS             complement(82919..83836)
                     /locus_tag="VFF22_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX19124.1"
                     /translation="MSTRRPDPLAQVSDFDIRLLKLFRSVVECGGFSAAESALGIGRS
                     AISQQMSDLEQRLGLRLCQRGRAGFALTEEGREVFQSTQQLLAALESFRTEVNGLHQH
                     LRGELNIGLTDNLVTVPHMRITNALARLKERGPDVHIHIRMTPPSEVEQGVLDGRLHI
                     GVVPQASALSGLEYQTLYDERSLLYCAVGHPLFYVDDQQLEDERLNAQEAIAPTFRLP
                     PEVQSHYQALNCTASASDREGMAFLILTGRYIGYLPDHYAQAWVQQGRLRVLKPTSRF
                     YDISLASVTRKGRRPHLVLESFLEALAEA"
     gene            84058..85404
                     /locus_tag="VFF22_19930"
     CDS             84058..85404
                     /locus_tag="VFF22_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZX19125.1"
                     /translation="MNMPQTETPSLASQLKLDAHWMPFSANRNFKRDPRLIVGADGNY
                     FIDDKGRRIFDSLSGLWTCGAGHNRKEIQEAVAKQLGTLDYAPGFQYGHPLSYQLAEK
                     ITELTPAGLDHVFYTDSGSECADTSVKMAKAYWRLKGQPSKTKFIGRARGYHGVNIAG
                     TSLGGIGGNRKMFGQMMDADHLPHTLQAHLAFTKGMAATGGVELANELLKLIELHDAS
                     NIAAVIVEPMSGSAGVIVPPVGYLQRLREICTQHNILLIFDEVITAYGRMGKWTGAEF
                     FGVTPDIMNTAKQVTNGAIPLGAVIASSEIYNTFMNQAIPEHMVEFGHGYTYSGHPVA
                     CAAGLATLELLKRDNLIEQSAALAPIFEDKLHSLKGSKNVIDIRNCGLAGAIQISPRD
                     GDPVIRPFEAGIKLWNAGFYVRFGGDTLQFGPTFNTTPEQLDSVFNAVGDVLNSLD"
     gene            85503..86999
                     /locus_tag="VFF22_19935"
     CDS             85503..86999
                     /locus_tag="VFF22_19935"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZX19126.1"
                     /translation="MTIVNHLIHGELTAGTGRSADVFNPSTGAATKKVALADRATMQL
                     AIDSAKSAFPAWRNTPPAKRAQVMFRFKQLLEQHEATISKLISEEHGKTLEDAAGELK
                     RGIENVEFACAAPEILKGEYSRNVGPNIDAWSDMQPIGVVAGITPFNFPAMVPLWMYP
                     LAIVCGNCFILKPSERDPSSTLYIAQLLIEAGLPKGVLNVVHGDKEAVDALIEAPEVK
                     ALSFVGSTPIAEYIYSEGTKRGKRVQALGGAKNHAVLMPDADLDNAVSALMGAAYGSC
                     GERCMAISVAVCVGDQIADALVEKIVPQIKALKIGAGTSCGLDMGPLVTGAARDKVKG
                     YIDAGVAQGASLVVDGRDLVVAGNENGFFVGGTLFDKVTPEMTIYTDEIFGPVLCIVR
                     VGSLEQAMQLINDHEYGNGTCIFTRDGEAARLFCDEIEVGMVGVNVPLPVPVSYHSFG
                     GWKRSLFGDLHAYGPDGVRFYTRKKAITQRWPQRASHEAAQFAFPSNG"
     gene            87389..88159
                     /locus_tag="VFF22_19940"
     CDS             87389..88159
                     /locus_tag="VFF22_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017739041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HZX19127.1"
                     /translation="MIGLPPCRWQLTNRDIGWFHLVGEKYIHAVTGFGAFPLMIPAFG
                     DDLDMDTVLDSVSGIMFGGSLSNVHPSFYGDDHPGLGLADKPRDETVLRLMRACIDRG
                     IPFLGICRGMQEMNVLFGGTLHREVHEVEGRLDHREVKDVPDDVAYGPMHNVALTEGG
                     VLHSLFGERQIKVNSLHGQGVDRLGEGLQVEAVAEDGQIEAVSVIGAKAFAVGVQWHP
                     EYRYWEKADYNALLGAFHQAAREYQVRKLERRSEAALV"
     gene            complement(88287..88637)
                     /locus_tag="VFF22_19945"
     CDS             complement(88287..88637)
                     /locus_tag="VFF22_19945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX19128.1"
                     /translation="MDEKIRNLIGTLTDKTSTSKIKWERTSRDNEFICKTGSGAITVD
                     SYMAGHEFSDEEEHVVDICILNDQGIQIERIYYDENSPSEFRLLLSLHQSIYRKYHKI
                     DEVIDNIISEISEL"
     gene            complement(88642..89199)
                     /locus_tag="VFF22_19950"
     CDS             complement(88642..89199)
                     /locus_tag="VFF22_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX19129.1"
                     /translation="MRIALIVSIIGIASGSYLLAGVTLTEFQNKYISIFGTLGTITGL
                     AYAIAQIALMRKESEVISETAKETKSQVLLLTCIADWARGVKVAQEVQIYCRSGKREI
                     AIPRIQELKQILQDIVNAANSEIKNTHGTNAANQIISLNIIINGFEKDTRAKMSGEEV
                     ARVNNTLEATIDLLNEIQSITKNKG"
     gene            90194..92377
                     /locus_tag="VFF22_19955"
     CDS             90194..92377
                     /locus_tag="VFF22_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZX19130.1"
                     /translation="MSDYPRPVNDGARIRALDALELMGSAPEREFDRVVQMAARIFDV
                     PIALVSLVHRDHQFFKARVGLDVCETSRELSFCTYAIMGQTAFVVADAHDDERFSANA
                     LVTGAPNIRFYAGFPLLTADGHALGSLCVIDQKPRHDFSVRDEQILRDLAALLSEYID
                     QRCFAREGDELRSRFRHIAATSPDGIICSNSRNLITSWNEAAEKLFGYSAQEAIGQTL
                     DLIIPAPMRGHHAAGVARVAGGGAPRLIGSSVNVHALRRDGSQFPIELSLSQWQEEGE
                     RCFGAIIRDITARTELEKQLKHAAEHDYLTGLANRARLKRCIQEALAAQQPATLLLID
                     LDGFKDVNDTLGHSAGDFVLQVVAARLREAVPLEQMVSRLGGDEFVVFVTGSTDQESA
                     RALGLELIGVIEEQIEFDDQPIFIGASVGVAIHCGTGESDEQLLGNADLALYQAKSDG
                     RHLVNIFNPELRQTASRKGAISSSMRQAWECQEFEMYYQPQVNLADGSLCGAEALIRW
                     NHPTQGVTAPAVFLPVLEAGLLAIPVGEWILRTACRQAAQWRSMGIGHFRIGVNLFAA
                     QFRARDFADMVKAILAEFNLPASALELEITENTILRNEQRIMQPLRHLRALGVGIAFD
                     DFGTGFASLSMLKDYPVSRLKIDRSFVSGADRSLRDEAIVEAVSRLAAGFQLEVIAEG
                     IETHEQARLMQHYACGEGQGYLYGRPMTADDFTRRYLGGRPVASE"
BASE COUNT        16910 a        27166 c        29469 g        19031 t
ORIGIN      
        1 aaaatcttcg attgggtgct gagctgggcg tgaatttcca gcccgattac ggtttcccat
       61 tgcatgtcag tcgtcctcag aatccgctcg gtgcgtgggt gtgccagtca gtgacctgtt
      121 ggtactggtg ggcgacgttg agcaggcggc cttcttggaa gtagttgccg agcaactgca
      181 cgccaaccgg taaaccatcg acgaagccgg caggcatcga caagccgggg atgccggcca
      241 ggttagcggt gatggtgtag atgtcttcca ggtaggcggc gaccggatcg ttgttcttct
      301 cgccgagctt ccaggccagg tttggcgtgg tggggccgag gatcacgtcg acgtcattga
      361 acgcagcgac gaagtcgttc ttgatcagcc ggcggatctt ctgcgccttg aggtaatagg
      421 cgtcgtagta accggcagac agcgcatagg tgccgaccat aatgcggcgt tttacttccg
      481 cgccgaagcc ttcgccgcgc gagcgcttgt agaggtcttc caggtttacc ggattctcgc
      541 agcggtggcc gaagcgcacg ccgtcgaagc gcgagaggtt gctgctggcc tctgccgggg
      601 caatcacgta gtaggcggga atggcgtgct gcatgttcgg caagctgatt tctttaatgg
      661 ttgcgccgag cttctttagc tcttcgacca ccgccatcac cttctcgccg atacgcgcat
      721 cgaggcccgc gccgaagtat tccttcggca ggccgatgcg caggcctgca agcggcagag
      781 tcagcgctgc taagtagtca tcaaccggct gatcaacgct ggtgctgtcc ttggtgtcga
      841 aaccggccat ggcttgcagc atcagtgcgc agtcttcagc cgtgcgggcc agtgggccgc
      901 cctggtcgag gctggaggca taggcaatca tgccccagcg cgacacacgg ccgtaggtcg
      961 gcttgatccc ggtgaggttg gtcagcgccg ccggctgacg gattgagccg ccggtgtcgg
     1021 tgccggtcgc tgcaggcagc aggcgtgcgg cgacagcggc agccgagccg ccggaagagc
     1081 cgcccggtac gcgggtcagg tcccaagggt ttttcaccgc gccgtaatgg ctggattcgt
     1141 tggccgagcc catggcgaat tcgtccatgt tcagcttgcc cagggtcaca gtgccggctg
     1201 cggcgagttt ctcgaccacg gtggcgttat acggggcttt gaaaccggtg aggattttcg
     1261 atgcgcagct ggtcagcacg tcttgcgtgc agaacagatc cttgtgaccg attggtgcgc
     1321 cgagcagggc gccgttctcg ccgttggcgc ggcgggcgtc agccgcagtg gcttggctca
     1381 gggccagctc ctcggtgacg gtgataaagc tgttgagctg cgggtcgagt tgggcgatgc
     1441 gcgccaacag gctgcgggtc agctcttcag acgaaaactg tttgtcggcg agtccgcggg
     1501 cgatctcggc cagggtaaat tgatgcatgg cttggcttat cccttactcg atgactttcg
     1561 gaaccaggta caggccgttt tccacggcgg gtgcgatggc ctgataggcc tcgcgctggt
     1621 tgcgttcggt gacctggtcg gcgcgcaggc gctgagtggc ttccagtggg tgagccagtg
     1681 gttcgatgcc gtcagtattg accgcttgca tctgatcaat caggccgaga atgttattga
     1741 gggtctcggt ggtacccggg atatcggctt cattcaggcc cagtcgggcc agatgagcga
     1801 ttttctccac gtcggagcgt tcaagcgcca tcaggtttct ccctatatga agcagcgcat
     1861 ttgctggccg cctttggaaa tgtgaacggg tcgccatgca caggtgcgca gggaacagat
     1921 agtgacaggt ggtggtcaaa ggtcgcgatg atgggcctta aagccctgaa aaaccggcaa
     1981 tctaacatat tggtgccttg ctcaaaatcc ctgccgttgt tagagtttgc cgcacttttt
     2041 agcagcgcct ttaccgcgct tcgcctttac ccacgcgttg cctagggtcc ttatcccatg
     2101 ttcaaaaaac tgcgtggcat gttttccagc gatctgtcga ttgacctggg cactgccaat
     2161 accctgattt atgttcgcga acgcggcatt gtgctgaacg agccgtccgt ggtcgccatc
     2221 cgtacccacg gtaaccagaa gagcgttgtg gcggtcggaa ccgaagccaa gcgcatgctc
     2281 ggccgtaccc ctggcaacat tgccgccatt cgtccgatga aagacggcgt gatcgccgac
     2341 ttcagcgtct gcgagaagat gctgcagtac ttcatcaaca aagttcacga aaacagcttc
     2401 ctgcagccgt cgccgcgtgt gctgatctgc gtgccgtgca aatccaccca ggtggagcgc
     2461 cgtgccatcc gtgaatcggc cctcggtgcc ggtgcccgtg aagtgttcct gatcgaagag
     2521 ccaatggctg ctgctatcgg cgccggcttg ccggtagaag aggcccgcgg ttcgatggtc
     2581 gtggatatcg gtggaggaac cactgaaatc gcccttatct cgctgaacgg cgtggtttat
     2641 gccgaatccg tccgcgtcgg cggcgaccgt ttcgacgaag cgatcatcac ctacgtgcgc
     2701 cgcaactacg gctcgctgat cggtgaatcc accgctgagc ggatcaagca ggaaatcggt
     2761 accgcctacg ccggtggaga actgcgtgaa gttgacgtac gtggccgcaa cctggccgaa
     2821 ggcgtaccgc gcagcttcac cctgaactcc aacgaagtgc tcgaagcgct gcaggaatcc
     2881 ctggcgacta tcgtccaggc ggtgaagagc gcgctggagc aatcgccgcc agaactggct
     2941 tccgacattg ccgagcgtgg tctggtgttg accggcggtg gtgccctgct gcgtgatctg
     3001 gacaaactgc tgtcgcagga aaccggcctg ccggttatcg tcgctgaaga cccgctaacc
     3061 tgcgttgcac gcggtggtgg caaggcgctg gagatgatgg atcgccacac catggacttg
     3121 ctgtcctcgg aataagcgcg accgctccac gtcttgagct gcaagccggg ttctactcgc
     3181 ttgcagcttt gttttgctgc ctgacgctgt gaggagctgc tgatcaaacc gctatttgcc
     3241 aaaggtcctt cgctgggtgt gcgcctgctg gtgttcaccg tgttgtcggc tgcgctgatg
     3301 gtggtcgatg cgcgctttac tctgctgcat ccgttgcgcg cccaactggg gctgattgtt
     3361 gagcctgtgt attgggttgg tcggctgccc gttcgggtgt gggaaagcgc gacccaagag
     3421 ctgagcacgc gtaacgagct tgccgccgaa aatgaaaagc tcaaggccga gcagttgatg
     3481 atgcagcgtc gcctgcagaa actcgctgcg ctcaccgagc agaacgtgcg tctgcgcgag
     3541 ctgctcaatt cggccgcgct ggttgacgac gatgtactgg ccaccgaact gattggtatc
     3601 gaccccaatc cctttaccca ccggatcctg atcgacaaag gtgaaaaaga cggtgtggta
     3661 ttgggtcagc ccgtgctgga tgcccgcggt ctgatgggtc aggtagtcga ggtcatgccc
     3721 tacacctctc gcgttctcct gctgaccgat accacccaca gcattcccgt gcaggtcaat
     3781 cgcaacggcc tgcgcgctat cgccagtggc accggtaatc cggagcgctt ggagttgcgc
     3841 catgttgctg acacggcgga tatcaaagaa ggcgatctgc tggtcagctc tggccttggc
     3901 cagcgcttcc cggccggtta tccggtggcc atggtctccg aagtgatcca cgactccggt
     3961 cagccttttg cgattgtccg tgcagtgccc accgccacgc tgaaccgcag ccgttacatg
     4021 ctgctggtgt tcaccgatcc gcgtaccccg gagcagcgcg ccactgagtc agcgcaggcg
     4081 caggaagccc tggaccgtga aattctgcag caggcgcaac ccgaacaacc tgcggtcccc
     4141 gcacccgcag cgcccgccaa tccgccggag gccacgcaat aatggccgca cgatcaagca
     4201 atatctgggt ggtctggctg acacttgctg tggcgctgct gcttagcgtg tcgagcatgc
     4261 ccaagttcat ggagctgggg cggccgctct ggttggcatt gtttctgacc tactgggtct
     4321 tggccgtgcc gcatcgtgtg ggcatgacta cggcctgggt tattggcctg ttagctgatg
     4381 tgctgaatgg caccttgctc ggccaaaatg cgctgatcct gactctgatt acttttctgg
     4441 tgctgaccct gcatcagcgt ctgcgcatgt tccccatgtg gcagcaaagc accgtgctgc
     4501 tggtggtgtt cggtttggcg cagctggtgc agctgtggct gaacgcgctg accggcagtc
     4561 gcccgccaac cttgaccttt gtcttgccgg cgctggtcag tgcgctgctc tggccttggg
     4621 tctgcacttt gctgcgcggt ctgcatcagc gtctgggcgt caattaagca aggccgctgg
     4681 gccttgcgtt tatccgacag ggagaagtca gcatggccac cctctacctc gcctccggat
     4741 cgccgcgtcg ccgcgagctg ctgacgcaaa ttgctgtgcc cttcctcacg caaatcgcac
     4801 cgattgatga aaatgcattg ccaggcgagt cgccaatcgc ctatgtagag cgtctggcgc
     4861 ttagcaaagc gcaagctgga ctggctgcat tgaccgatac cgcggatgcc gtggtgctcg
     4921 gtgcagatac ggcagtggtg cttgatggac gaattcttgg caagccgacc gaccgtgccg
     4981 atgcgctggc gacgctcagt gatctgtctg ggcgaaccca tgaggtgctg acggccgtgg
     5041 cgctggtcag ccgcgaacgc caggtgtcgc gggtggtcac aagccaggtg accttccgcg
     5101 cgttgagtca ggctgaaatc gaggcttact gggccagcgg cgagccgcag gacaaggccg
     5161 ggtgttacgg tattcagggc ctggctgcgg tgtttgtcag ccagttgcag ggcagttatt
     5221 cggcggtggt gggtttgccg ctgtgtgaaa ctgccgcgtt gctcgcagaa tttgctattc
     5281 cgtgctggca gtcgctgcca gttcacagtc aagaggttgc gggtcgcggg tgagtgaccg
     5341 ccattatcgg cgcagtccct gggctttcag ctttatctca gaagagatga gcgcatgagt
     5401 gaagagatcc tgatcaacat cacgccgatg gaatcgcgcg tggcggtggt ggaaaacggc
     5461 gtactgcagg aagtgcatgt cgagcgcacg cagaagcgtg gcatcgtcgg caatatctac
     5521 aaaggcaaag tggtgcgcgt actgccgggc atgcaggcgg cctttatcga tatcggcctc
     5581 gagcgtgcgg cgtttattca cgcggcggaa atttccaccc gtgaaggctc ggcggtggaa
     5641 agcatcagcg ctctggtgca tgaaggccag agcctggtcg tgcaagtgac caaggatccg
     5701 attggcagca agggcgcacg tctgaccacc cagctgtcga ttccttcgcg ctatctggtc
     5761 tatatgccgc gcaccgccca cgttggcatc tcgctgaaaa ttgaagacga agccgagcgc
     5821 gagcgcctca aacaggtggt ggccgattgc gtggcggcag aaggcatcga ggaagccggt
     5881 ggttttattc tgcgcaccgc ggctgacggt gccggtgccg atgagatcct tgcagacatc
     5941 cgttacctgc gccgcctgtg ggaacagatc gccacgcaga tgaaggtcgg gccgacaccg
     6001 ctggtgatct atgaagacct gtccctagcg ctgcgcaccc tgcgtgacct ggttaacccg
     6061 aaaatcgaga agattcgcgt cgattcgcgc gaaaccttcg gcaagatcac tcagttcgtt
     6121 gatgagttga tgccggaaat cgctgaccgc ctggagcact atccgggtga aaggccgatc
     6181 ttcgatctgt atggcgtcga ggatgaaatc cagaaggcgc tggagcgcaa ggtgccgctc
     6241 aagtccggtg gttacctgat cattgatcca gccgaggcga tgagcaccat tgatgtgaat
     6301 accggtgcgt tcgtcggcca ccgcacgctg gaagagacca tcttcaagac caatctggaa
     6361 gctgctaccg cgatagctcg ccagctgcgc ctgcgcaata tcggcgggat catcatcatc
     6421 gacttcatcg acatggaaga tgaagagcac cagcgccagg tactgcgcac cttggagaag
     6481 cagctggagc gcgaccacgc caagaccaat atcatcggca ttaccgagct gggcctggtg
     6541 cagatgaccc gcaagcgcac ccgcgagagc cttgaacagg tgctctgcga gccatgcagc
     6601 gcctgtcagg ggcgcggcaa gctgaaaacg gcggagacca cctgctacga aatcttccgc
     6661 gaaattctgc gtgaggcgcg ggcctatcaa ccggaaggtt atcgcgtgtt ggccaatcag
     6721 aaagtagtcg accgcctgct cgatgaagag tcgggcaacg tcgccgatct cgaagctttt
     6781 atcggccgca ccatcaagtt ccaggttgaa accatgtatt cccaggaaca gtacgatgtg
     6841 gtgctgctct gatatgcgcg tttaccgcct gggcgtgaac tgaatggccg cgttggcgcg
     6901 ctgggttacc ctgctgctgc gtacggcatt ggggctgtgc gctttgacgc tggtattggc
     6961 ggcgctgtat gtcagcctcg ggcgcgagct ggtgccgctg gtcgccgaat accgtctgga
     7021 agccgaagac aaggcccgtc aagcgttggc catgccggtg cagatcggtg aattggaagg
     7081 cctctggcag ggtttttcac ccgtacttac tgctcacgat gttgcgctcg gtgagggcgc
     7141 aggggccttg caactggatc aggtgcgcgt agtgcctgat gtgctggcta gcctgttggc
     7201 gcgtcagttg cgcattgccc ggcttgagct ggatggcgtg cagctggtcg tgcagcaagc
     7261 tgaagacggc agctggtcgc tcaagggctt accgcaacgc aatgaacccg cgccgctcga
     7321 tctggctcag gtgctgacac agtcgcagat gctcaagcgc ctgtcgctgt ttaacagcca
     7381 agtcaccatg caggccttcg atcagccggc acgaaccctg acctatgtaa acctcaccct
     7441 gcgtaacggc agcgcgcgtc agcgcctgga cggccgcctg ctgttgcccg atggccagcc
     7501 gcttgccctg cagttgcgca cgcatataaa cgccaagcac tggcaagacg cccaagctga
     7561 gctgtatctg agcctgccgc agagcgactg ggcggcctgg gtgccgacga gcctgttggc
     7621 gcaatggcgg cttgagcagt tgcagctcgg tggtgaggtc tggctgagct gggccaatgg
     7681 cggcgtgcag cgggctgtca gccgcctgca tgtgccgcag ctcaaagccg gctacgccga
     7741 gcgcgagtcg gtggcgctga ataatctggc gctgaatgct tacttcaccc gcaccgagca
     7801 agggttcaag ctgctcctgg atgatctggc gttcagtcgg ggtgaacagc gttggggcga
     7861 tgtccgtctt gcattgaccc agcaggcaga gagcgcagaa gtacaggaac aatggttgct
     7921 gagtgctgat cgccttgatc tggcgccgct tcagcccttg gtggcagggc ttgcaccact
     7981 gccggataag gggctggcgc tacttgaaca actcaaaccg catggcgcgc tgcgcaatct
     8041 tcaactcgac taccggccgc ggttgagaga cggcaagcgc ctgcagttct cggccaacct
     8101 agaacgcatc ggttttgccg cataccttgg ctcgccagcc gcagaaaacg tcagcggcag
     8161 catcagcggt gatctcggtc agggtgagtt acggctggac agcgaagatt tttccctgca
     8221 cctcgacacc ctgttcccta aaccttgggt ctaccagaaa gccaacgcct tgctgacctg
     8281 gcagattgac gagcacgcct tcaccttacg cagcccgtac ctgcgtgtgc agggcgagga
     8341 ggggccggtc gccggtgact ttctcatccg cctgcgtgtt gatccgacgc aggaagatta
     8401 catggacctg cgtgtcgggc tgcgcaacgg cgatgcgcgc tttaccgaga aatacctgcc
     8461 aagccgtgcg ccgggcatga gtgccgactt ggatgcctgg ttgaaaagtg ccatccgggg
     8521 tggcgcggtg gatgaaggct atttccaata ccagggttcg ctgaacaaag gtgcagaaaa
     8581 tgctgcgcgc agcatcagcc tgttctttgc tgtgcatgat gccgagttgg cctttcagcc
     8641 cggctggcca gccctgcgtg aaggccgtgg tcgcgtcttg atcgaggaca ccggcgtacg
     8701 cgttgagttg gccgaagggc gcattctcga tagccaggtg agcgatgctc aggcattgat
     8761 tgcgacggca ttgccgggcc agccgccgcg tctgttgctg accagtgagc tgagcagcaa
     8821 cgttgaggat gccctgcata tcctgcaaca ggcgccgata ggcactgccg aaacgtttgc
     8881 gggatggacg ggtgctggtg cgctggctgg caagctcaag ctcgatctgc cgctgcgcaa
     8941 gggcttagct ccggcagtgg tggtggattt cgctactgaa agcgcacaac tcagcttgcc
     9001 caacccgccg ctgcaattca gtcagttgca aggtgccttc cgttacaaca ccgccagtgg
     9061 cctgagcgct ccggatattc gtgcccaagt gctggggcat gcggtgcgcg gcaaagcgtt
     9121 ggccgaaggc gcgcggggta aagcacgcag ccgtattgag gccagcgggg agattgccgt
     9181 caaagaactg agcacttggc tgggggtgac ccagccgctg cccatcagcg gtatgctgcc
     9241 gtatcgcctc aacctgattc tcgagggggc cgacagccaa ctgcgcgtga gttcatccct
     9301 caaaggcgcg acggtggcct tgcctgcacc gttcggcaag gccgctgccg aggcgcgggc
     9361 gacgcagtgg cgcatgagtc tgggcggtcg cgagcagcgc tactggttgg attactccga
     9421 tttactgagc ctgaccttcg ctgctagccc aggccagttc aatcaggggc gtggtgagat
     9481 tcgcttggcc gacggtccag ccagcctgcc taccagccaa ggcttgcgcg tgcgtgggcg
     9541 tgtggcagag ctggattggt cggcctggca ggcggtgttg cagccctatg ccagcgtacc
     9601 gatcggtgat gcacagcgac tgttcaagga tgcacagctg cgtattgggc gtttcagcgg
     9661 ctttggcatt agtctcgata cgctggaggt cgcggttcag cgtgaggctg ccacctgggc
     9721 agtcagcctc gacagcccgc agctcaaagg ccaggtcggc ttgccggatg cccgtgagct
     9781 gccgattgct ttgacactcg actatctgcg cttaccagcg gcagagaaga aaatcgccgg
     9841 cgaggctgtg gtcgatgcgc ccgatccgct ggccagtttc gatccgagcc gtttcccggc
     9901 gctggacgtg cgcatcgccg aggtctatca aggcatcgaa ccgctgggca gctggtcgct
     9961 caaggcgcgc ccggtcagtg ccggtgtgca tttcaacgag ttggacctca acctcaaagg
    10021 cctgaagatt ggcggccgcg ccacctggct aaacgccgca aatggcgtgc gcacgagcta
    10081 caaggggcgc atggaaggtg ccgatctggc cgatgtgctg atcgcctggg gctttgcacc
    10141 gacggccagc agccgcgact tccatatgga tgtcgagggt gattggcccg gctcgccggc
    10201 ctggttcggc ttgaagcgtt tcagcggtac gctggatgct tcgctgcgca aaggtcagtt
    10261 ctcagaggtg gaagggccgg cttcggcgtt gcgggttttc ggcctgttga atttcaactc
    10321 gatcggtcga cgcctgcgcc tggatttctc cgacctgctt ggcaagggcc tgagctatga
    10381 ccgagttaag ggattgctgg tggccgataa cggtgtattc gtgacccgtg agccgatcac
    10441 ccttgaaggc ccatcgagca atctggagct taatggcact ctggatatgg ccaatgaccg
    10501 tatcgatgcc aagttgttgg tgaccttgcc ggtgaccaac aacctgccac tggctgcgct
    10561 gatagtcggt gcgcctgcca tcggtggtgc gctgtttatc gccgataagc tgctggggga
    10621 ccgtgtggcg cgctttgcaa gtgtgcagta cgacgtcaag ggtgcctggc agaatccgga
    10681 catcagcttc gacaaaccct tcgaaaaacc tcagtaaacc gtgcatggct gatatgcagc
    10741 cgtgcagtct gcaggtatgg cttgtaagct gaagccatac cgacattgag ataaaggcag
    10801 tcatgacttt cgctgtcatt caaatggtca gccaggccga tgtgctggcc aatctgatcc
    10861 aggctcggca gctgcttgag caggctgccg cacaaggtgc gcgattggcc gtgctgccgg
    10921 aaaatttcgc ggccatgggc cggcgcgatc aggccgcgct ggggcgcgcc gaagcgttgg
    10981 gcgagggacc gattctgcct tggttgcaac aagccgcccg tgacctggac ctgtggattg
    11041 tcgccggcac cttgcctttg ccgccggatg gtcagcccga tgccaaggcg catgcctgct
    11101 cgctgctgat cgataatgcc ggccagcgcg tggcgcgcta tgacaagctg cacttgttcg
    11161 atgtggatgt cagcgacagc catggccgct accgcgagtc ggatgattac gccttcggcg
    11221 ctcaggtcgt cgttgccgat actccggtcg ggcgcttggg tatgaccgtt tgctatgacc
    11281 tgcgctttcc cgagttgtac agtgccctgc gcgcagccgg tgcagagctg atctctgcgc
    11341 ctgcggcctt tactgcggtt accggtgcag cacattggca gatactcact cgcgcgcggg
    11401 ccatcgaaac ccagtgctat gtgctggcgg ctggacaggg cggcacccat cctggcgggc
    11461 gcgaaacctt tggtcattca gcgctggtcg atccctgggg ccgtgtgctc gcggagctgc
    11521 cgcagggggt gggcgtactg ctggccgagc gtgatgcaac tgagcaggcg gcgatccgcc
    11581 agcgcatgcc ggtggcgcgg cacaaacgat tttttgcggc agccgaagca cggctgccag
    11641 gtgcaatagt catcaacgag attgagcaat gaacagtatg ttggtatccg tcagtgaaca
    11701 tctgctgggg ccgggcggct tgaccattga gcacttgcct gcggtcctgg ctgacttggc
    11761 cgggccggga atcgatgcgg ccgacctgta cttccagagc caggtctcag aaacctgggc
    11821 gctggaagac ggcatcgtca aggaaggcag tttcaacctc gatcagggtg tcggtgtacg
    11881 cgctcagtcg ggtgagaaaa ccggttttgc ctacagcaat gcgatcactg ccgatgccct
    11941 gagtcaggct gcgcgagcgg cccgttcgat tgctcgtgct gggcagaacg gtcgtgtgca
    12001 agcgttcagc agcccgctgg tcaaaccgct gtatgcccag gacaacccgc tggatgtgct
    12061 ggggcgggcg gaaaaggtcg agctgctcaa gcgcatcgac gttgccacac gcgcccttga
    12121 cccccgcatc aagcaggtct cggtcagctt ggccggagtg tgggatcgcg ttctggtggc
    12181 ggcgaatgac ggcagcctgg gcgcggacat ccgcccgctg gtgcgcttca atgtcagcgt
    12241 gatcgtcgag cagaacggtc gtcgcgagcg cggcagccaa ggcggcggcg ggcgtaccga
    12301 ttaccgctac ttcctcgaac agaccagcaa taacgaagag cgcgccatgg gctatgcccg
    12361 tgaggcgctg cgtcaagcgc tggtcaatct ggaagcgatt ccagccccag caggcacgat
    12421 gcccgtggtg atgggcgccg gttggtctgg cgtgctgctg cacgaggccg tcggtcatgg
    12481 cctggagggc gacttcaacc gtaaaggcag ttcggcctac agcggccgca tgggcgagca
    12541 ggtggcgtcc agcctgtgca ccatcgtcga cgatggcacc ctggccgagc gccgtggttc
    12601 cctgagcatg gacgatgaag gcacccagac ccagtgcacc acgctgatcg agaacggcgt
    12661 gctcaagggc tacatgcagg acaagctgaa tgcccgcctg atgggcgtgg cctgcaccgg
    12721 taacggtcgt cgtgagtcct acgcgcacct gccgatgcca cgcatgacca acacttacat
    12781 gctggccggc gagagcgacc cggaagaaat cattcgttcg gtgaagaagg gcatctactg
    12841 cgccaacctt ggcggcggtc aggtggatat caccagcggc aagttcgtct tttctaccag
    12901 cgaggcttac ctgatcgagg acggcaagat caccgccccg gttaaaggcg ccaccctgat
    12961 cggtaatggc ccggaagcca tgagcaaggt gtcgatggtt ggtaacgacc tggcgctgga
    13021 cagcggcgtt ggcacctgcg gcaaggacgg ccaatcggtg ccggttggcg tgggccagcc
    13081 gacgctgaag attgatgcga ttacggtggg cgggacaggc agctaaaaac cgtcgattgg
    13141 gttgagcgtt gcgcagccca atcgatgtgc tggtctagcg caagccgcgc tgggtttcgt
    13201 ccagctcgcg gatgtatttg aagattttgc gggacgcggc aggcgctttg ttctgcgcca
    13261 gttcgtgctg gccctgacgg atcagctggc gcaggtgctg gcggtcggcg tcggggtatt
    13321 cgacgacaaa tttttccagg gtgtcgtcgt cgccgttgag caggcggtca cgccagcgtt
    13381 ccaggttatg aaaacgttcg ttgtactggc gggttgaggc atccagctgg tcgagcaggg
    13441 tcaggatggc aggaatgtcc tgatcgcgca tcaagcggcc aataaactgc acatggcgct
    13501 tctttgcggc attggcggtg tgtttgggcg cttcggccag cgcgcggcgc agctcgtcgg
    13561 tcagcggcag tttgttcagc agatcgagtt tcagggtagt aaggcgttgc cccagatcct
    13621 gcagcgcatg aaactcgcgc ttgatctggg ttttgctctt ctcgccggag aagtcgtcgt
    13681 caaggtattc agacatggtg gcggtccagt ggaaaacgcc gacatgatag cagcaagttt
    13741 ccagctgtaa gcgtctggcc tgtgtggccg cgtttgccgc ttatcgttca ggagttctta
    13801 tgagtgcagt agacatcgtc ggcccgcagg ctgtgcccca tttacaggcg caggtggagc
    13861 agattattgc cgaggctaaa aagcagggcg cgagtgcctg cgaggttgcc gtctcggtgg
    13921 agcagggctt gtccaccacg gtgcgtcagg gtgatgtgga aacagtcgag ttcaaccgcg
    13981 accaaggttt tggcatcacg ttgtatgtgg ggcagcgcaa gggctcggcc agcacttctg
    14041 caagtggtga ggcggcgatc cgtgaaacgg tcgcggccgc cctggcgatt gccaaacatg
    14101 cctctgaaga tgagtgtgcc ggcttggccg atgccgcact gatggcccgc gagctgcccc
    14161 agctggatct gtaccacgcc tggtcgatca cgcctgaaca ggccatcgag caggccttga
    14221 gctgtgaggc tgcggctttt gctgccgatc agcggatcaa gaacgccgac ggcaccacgc
    14281 tgaataccca tcaaggctgc cgggtttatg gcaacagcca cggctttatc agcggttacg
    14341 ccagcacgcg gcacagcttg agctgcgtga tgatcgccga agacggcggg cagatgcagc
    14401 gcgactattg gtacgacgtt aatcgccagg gtgagttgct ggccgatgcc agcagcattg
    14461 gccgtcgtgc cgccgagcgt gcggtaagcc ggttgggtgc gcgtccggtg ccgacctgcg
    14521 aagtgccagt gctgttcgct gcagaattag cgggtggcct gtttggcagt tttctcgcgg
    14581 cgatttccgg cggcaatttg taccgcaagt cgtcgtttct tgaagggaca ttaggtcagc
    14641 agttattccc tacgtggttg acactggatg agcgtccaca tatcccccgt gcgctgggca
    14701 gcgctgcctt tgatggcgac ggtctggcga cctatgcaaa atcctttgtc gagcgcggcg
    14761 agctgctgtc ctatgtgctc ggcacctact ccggacgcaa gctgggtatg cccagcaccg
    14821 ccaactccgg tggggttcat aatctgtttg taagccatgg cgatgaagac cagaaggcgc
    14881 tgctcaagcg catggggcgt ggtctgcttg tcaccgagct gatgggccag ggcctgaata
    14941 tggtcaccgg tgattattcg cgcggtgccg gtggctactg ggttgagaat ggcgaaatcc
    15001 agttcccggt gcaggaagtc acgattgccg gcaacctgcg cgacatgttc aagcagattg
    15061 ttgcagtcgg taatgatctg gaactgcgca gcaatatccg cacaggttcg gtgctgattg
    15121 agcggatgac tgtggcgggt agttgattcc gcttgcatga aaaaaggggc tgtcgcgtaa
    15181 gcgttcagcc cctttttact gcctcacaat cagctattcg ccttcgtcga agtagttgtt
    15241 gatcagctcg ctcagggcct gcagcgcctc gctatcctgc tcgccttcgg tgtgcaggtg
    15301 caccggtgtg cctttgccgg ctgcgagcat cattactgcc atgatgctct tgccgtcgac
    15361 caggctctcc ggcgtacggc ctaccttaac ctggcaggga aaacgcccag cgacgccgac
    15421 aaacttggcc gccgcacggg cgtgcaggcc aagcttgttg ataatgatga tttcgcaggc
    15481 gggcatcgcg gcaattcctt agctgaggtc gcggtggcga gtctgaacgt ttttcaggat
    15541 gggtttgagg gtttccccca ggcggttggc caggtacacc gagcggtggt ggccgccggt
    15601 gcagccaatc gccaccgtga cataggcgcg gttgctggcg gcaaagcgcg gcagccattt
    15661 ggtcaggtag gcgaggatgt cctggtacat gtcttcgaca tcggcctggg ccgagaggta
    15721 gtcgactacc ggctggtcga ggccggagaa gtcgcgcaag tccggcttcc agtagggatt
    15781 gggcaggcag cgcacatcga ataccagatc agcatccacc ggcatgccgc gcttgaaacc
    15841 aaaggactcc agcagaaacg cggtacccgg ctcgggttgg ttaagcaagc gcagtttgag
    15901 gctgtcgcgc agctggtaca ggttgaggtg ggtggtatcg atcttcaggt cggccaggtc
    15961 gatgatcggc ccgagcagca ggctttcatc agcgatggct tcggccaacg aacggctctc
    16021 gttggtcagc ggatggcgcc ggcgggtttc cgagaagcgc ttgagcaggg tttcttcgtc
    16081 ggcatccagg tagatcacat cgcacttgat gtgccgcgcg cgcacctcat cgagcaactc
    16141 gggaaagcgc ttgaggtggc tcggtaggtt gcgtgcatca attgatacgg cgacctgcgg
    16201 atgcagcagt tcggtgtgca gcagggcgcg ctcagccagt tcaggcagca gcccggctgg
    16261 caggttgtcg atgcagtaga aaccgttgtc ctcaagcaca tcgagggcgg tgctcttccc
    16321 agagccggag cgaccgctaa cgatgatcag gcgcatgttc aggctcggtt gagttggctt
    16381 tgtacgtcca ctaccacctg atacagggct tcgctgcttt ccgcttggcg caggctggca
    16441 cgcacatcgc tgcgatcgag catgctggcg atctgccgga gcagttccaa gtgagcgtcg
    16501 gtggcagctt cgggcaccag caggacaaac agcaggtcca caggcgcacc gtcgatggcg
    16561 tcgaagtcta cggcagcgtc caggcgcagc aaggcgctga tcggcgcggt acaacccggc
    16621 aggcgacagt gcggaatggc aatgccattg ccgaagccgg tcgagccaag tttttcccgg
    16681 gctatcaggc tttcgaagat gtcttggccg tccaggtcag gtagctcacg ggccaccagg
    16741 ttggcaatct gttcgaggac acgttttttg ctgccgcccg gcacgttcac taaagaacgg
    16801 ccgggggtca ggatagtttc aagtcggatc ataggagata gacgatgtca gcgggccata
    16861 gcgccctgtt gacgttcgag ctgtttttcc ttgtgtttga tgagttgtcg gtcgagttta
    16921 tcagtcagta gatcgatggc tgcgtacatg tcttcatgtt cggcattggc gacgacttca
    16981 ccgccagcaa tgtgcagggt cgcttcgatt ttctgtttga gcttctcgac ctccatggtg
    17041 acttgcacgt tggtaatctt gtcgaagtgg cgctctaacc ggccgagttt ttcggcgatg
    17101 tagtcgcgca gggcgtcggt cacatccagc tggtgtccac tgatgttgac ttgcataccg
    17161 ctttctcctt gttgccgtgt gtaagaggca ggcatgaggc ctaccgccgg aacactgtgg
    17221 cgtggaagac tcgggcagaa cgagcctggc acggtcgctg attgcatgtt ggcagtatgg
    17281 ctgcttttgc gaaaaggcga ggcctgggca tgttctgtct gggttgatgt cttccttctt
    17341 tgtgccgcct tagatcaatc gtttgcgctc actggaaggc gctattccga gggattcacg
    17401 gtacttggcg accgtgcggc gagcgacttg aatgccctgt gcttccagta aaccagcgat
    17461 cttgctgtca ctcaacggct ttttcgcatt ttccgccgcg accagttttt tgatgatggc
    17521 gcggatcgcg gtggacgagc attcgccgcc ctcggaggta ctgacgtggc tggagaagaa
    17581 gtatttcagc tcgtaaatgc cacgcggggt gtgcatgaat ttctgcgtgg tcacccgtga
    17641 aatggtcgac tcgtgcatgc ctacggcttc ggcaatatcg tgcagcacca gaggtttcat
    17701 cgcctcgtcg ccataatcga ggaaaccacg ctggtgttcg acgatctgcg tggccacctt
    17761 catcagagtt tcgttgcggc tctgcagact tttgataaac cagcgcgctt cctgcagctg
    17821 gttacgcata aaggtgttgt cggcgctgga gtcggcacgc ttcacaaaac cggcgtattg
    17881 cgcgttgacc cgcaggcgcg gcatggcttc ttggttcagc tccaccagcc agcgctcgtt
    17941 gtgtttgcgc acgattacat cgggcaccac gtattccggc tcgctggatt cgatctgcga
    18001 acctgggcgc gggttgaggc tttgaataag gtcgataacc tggcgcagct cgtcttcctt
    18061 gagcttcatg cggcgcatca gctggctgta gtcgcggctg ccgagcaggt cgaggtagtc
    18121 gctggccagg cgctgggctt cactcagcca tggggttttc gctggcagct gacgcagttg
    18181 caggagcagg cactcgccca ggttgcgcgc ggcgataccg gtaggctcga actgctggat
    18241 gcggtgcagc acggcttcga cttcgtcgag ctcgatgtcc agttcagggt cgaaggcttc
    18301 gaccacttct tccagggttt cttccagata gccctgttgg ttgatgcaat cgatcagggt
    18361 gacgccaatc aggcgatcct tgtctgacat cggtgccagg ttgagttgcc agagcaggtg
    18421 gctttgcagg ctctcgccgc tggaggtgcg ggtggtgaaa tcccactcgt cgtcatcgtt
    18481 ggtgctgctc ggtaggctgc tggcgctggt ctggtaaatg tcttcccagg cagtgtcgac
    18541 gggtagctca ttaggaatgc gctcgcccca atcgccttct tccaggttgt ccacggtctg
    18601 cgtggcttcc tggtagttgg tttcctgata gggcgcttcg ggctgttcgc tggggctggc
    18661 gctttctgcg ccatcggcca tagggtccga attgtcgaaa tcatcgccat cttcctggcg
    18721 ttcgagcatc gggttggact ccaaagcctc ctggatctct tgctgcagat ccagggtgga
    18781 cagttggagc aggcggatgg cctgttgcag ctgcggtgtc atcgtcagct gctggcccat
    18841 tctcaggact agcgatggtt tcattgcaga ccttatttac cggcgtctgt gcgcgatcca
    18901 ctacagggcg tttttaaaaa gcgccactag gaagcaaatt atatgcctga ctttaaaggt
    18961 tttgcctagg cttggctgca gcttgcttga gaaaagctgt ggttacagcc ggaactcatg
    19021 gcccaggtaa acttctttca ccgtctggtt ggccagaata gtctcggcat caccttcggc
    19081 gatcagttgg ccatcgctga cgatataggc ggtttcgcag atatccaggg tctcgcgcac
    19141 gttgtggtcg gtgatcaata cgccgatgcc cttggccttg aggtggtgga tgatctgctt
    19201 gatgtcgccc acggaaatcg gatcaacacc agcgaacggc tcgtcgagca ggatgaactt
    19261 ggggttggtc gccagagcgc gggcaatttc tacgcgacgg cgttcgccac cggacaggct
    19321 catgccgagg ttgtcgcgga tatggctgat atggaattcc tgtaacagcc cctcaagctc
    19381 tttgcggcga gctgcgctgt cgaggtcctt gcgggtttcc aggatggcca tgatgttgtc
    19441 ggccacgctg agcttgcgga aaatcgaggc ttcctgcggc aagtagccga tgccggcgcg
    19501 ggcgcggcca tgcatcggct gatggctgac gtcaaggtcg tcgatcatca cacggccctg
    19561 atcggcctgt accaggccaa cgatcatgta gaaacaggtg gttttgccgg cgccgttggg
    19621 gccaagcagg ccgacgatct gcccgctgtc gatgctcagg ctgacgtcgc gcacgacctg
    19681 gcggcctttg tagcttttag ccaggtgttg ggctttcaat gtggccatta ttactgcgcc
    19741 tgctggtcgg ctggtttgtt cttcggctgg atcaccatgt cgatgcgcgg acgtggcgca
    19801 gtgatattgg tgcctgttgc gcggcctgcg ttgacgatct ggcgctgggt gtcatagacg
    19861 attttctcgc cttcgaaggt gttgccttcc tgaatcacct tggcctggtc gatcagtact
    19921 acacgatcat tggccataaa gtactggatg gtcaggccat aagccttgac gatctgctta
    19981 tcggctgacg gcagttgctc gtaataagcc ggcttgccga ccgaggtgaa tacctcgaca
    20041 tcgccctgag cattctgtgt gatggtcacg gtgtcgccgg taaccttcat ggtgccctgg
    20101 gtgatgacga cgtcaccgcg ataaacggcc acgccttgtt tgtcatccag ttccgcgctg
    20161 tcagcctgga tacggatagg ctgttcgcgg tcggacggca gtgcccaggc gctgacactt
    20221 ccgagtgcgg cgctgaggct gagtagtagg gggagggtat taacgagcct catgctgacc
    20281 tcttacgttg gacagcagga gcatcctgcc gtcattcatg tacgctttca ttccttttgc
    20341 cgtggtcacc ccattggccg cgtcgattct aacggcttgc tgggtctgcg catattcctt
    20401 ctcggggaat acggtcaggc ggctggtggt gaggatggtc gggcggcctt tggcgtcggt
    20461 gcgttcaact cgcaccttgt cgatcagttc gacttcaacg ccgcctggcg acacttcacc
    20521 gcgctcgctg cggatttccc aaggcagctc ggtgccgcgg aacaggttca tgtttgggct
    20581 ggtcatcagg gtgatgtcgc tggccttgac gtgctcgacc ttgtcggccg tcagctcgta
    20641 gtgcagcttg ccatcggcct ggtattgcac ggtataggtg ttggtggcgt aaaaatcgat
    20701 ggtgttgtca gttcctgcgc cacgcgattg ctcgttaaag ccttctgggc tgaggttcca
    20761 gtagccgagc gccgccacca gcagtgcggc aaaggcgaac agggcaatat tgaataactt
    20821 gcggggcatg gttggcctat aggtaagcgg cttgagcggc ttcaagggtg ccttgggcgt
    20881 gcattatcag ttcgcagaac tcacgggcgg cgccttcgcc accgcgagca cgagtaactc
    20941 cgtgggcgtg ctggcggaca aagccgtcgg cgctggcaac tgccatgccc aggccgacgc
    21001 ggcggatcac cggcagatcg ggcaggtcgt cacccagata agcgacctgg gcatagtcta
    21061 ggcccagttc gccgagcagt tcatcgagta ccactagctt gtcttcgcgg ccttggtaca
    21121 ggtgttgaat gcccaggttc tgcgcgcggc gctcaaccac cggcgttttg cggccgctga
    21181 taatggcggt gcgcacgcag gagttgatca gcatcttgat gccgtggccg tcgagggtgt
    21241 tgaaagtctt gaactcgctg ccgtcgacga ggaagtacag cttgccgtcg gtgagtacac
    21301 cgtcgacatc gaagatcgcc agtttgacgg cttgggcgcg ttgcagcagg tcggagttga
    21361 tctggtcagt ggtcattaca ttaccccggc gcggagtagg tcgtgcatgt tcaaagcgcc
    21421 gattggcaga tcgttgtcgt cgatcaccac cagtgagctg atcttgtggt cttccatgat
    21481 ttttagagcc tctgccgcaa gcatttccgc gcgggcggtc ttaccgtggg cggtcattac
    21541 gtcatcaata gtggtttggc gcacatcaat gttgcgatcc agtgtgcggc gcaggtcgcc
    21601 gtcggtgaat atccccgcca aacggccatc gtcctcggtt accacggtca tgcccagacc
    21661 tttttgggtc atttccagca gcgcgtcacg cagcgaagtg ccacgtttga cgcatggcag
    21721 gctgtcgcca gcgtgcatga cgttttccac cttgagcagc aggcgtcgtc ccagtgcgcc
    21781 gcccgggtgg gagaaggcga aatcttcagc ggtaaagccg cgggcttcca gcagggcaat
    21841 ggccagggca tcgcccagaa ccagggacac tgtggtggag gaggttgggg ccaaatttag
    21901 cgggcaggct tcctgggaca cgcgggcgtc taggttgact tctgcggcct tggccagcgg
    21961 cgactccggg ttgccggtca tgctgatcag gcggatgccg aggcgcttga tcaggggcag
    22021 tagggtgacg atttctgcgg tggagcccga gttggacagg gccagcacca cgtcatcgcg
    22081 ggtgatcatg cccatatcgc cgtggctcgc ctcggccggg tgcacaaaaa acgctgtggt
    22141 gcctgtgctg gctagggtgg ctgcaatctt gttgccgacg tggccggatt tgcccatccc
    22201 gaccactact acgcgacctt tgctggcgag aatcagctca caggcacgca cgaaatcacc
    22261 gttgatgcgc gcgagcaagt cctggatggc ttcgagctcc aagcggatgg tgcgctgcgc
    22321 tgactggatc agatcggttg actggctcat gaattctctg cggcgtgcgg gctaaaagct
    22381 gccgattata acggtaaagg tgcgcctgcc cagtgtttcc agtcgtcagt tgctgcttgg
    22441 aagtgtgcgg tgcttatctt cggcaacacc ttcagtcttg ggcggggaat gccgcggtgg
    22501 tatagttcgc ggccattttc ggtacgttcg acgaccagta gtgccctttg ttcagggcgg
    22561 tacgtcagtc aggcaagcgt tgagcaagga gtccagatga gcgccgagaa cccgtatgca
    22621 gttgagctga agaacgtcag ctttaaacgc ggtgagcggg atattttcaa aaacatcgat
    22681 atccgcattc cccgtggcaa ggtcaccggg attatggggc cgtctggctg tggcaaaacc
    22741 acgctgttgc gcctgatcgc ggctgaactc aaaccgagcc agggtgaggt ctgggtcaat
    22801 ggcctgaatt tgccgacgct gtcgcgcagc gagctgttcg atatgcgcaa gcagatgggc
    22861 gtgttgttcc agagtggtgc gctgtttacc gatctggatg tgttcgagaa cgtcgccttt
    22921 ccgctgcggg tgcacaccac gttgccggaa gagatgattc gcgacattgt gctgatgaag
    22981 ctgcaggcgg tgggcctacg tggcgccctg gagctgatgc cggacgagct gtcgggcggt
    23041 atgaagcgtc gcgtggcgct ggctcgggcg attgccctag acccgcaaat actgatgtat
    23101 gacgagcctt tcgtcggcca ggacccgatt gccatgggcg ttctggtgcg tctgattcgc
    23161 ttgctgactg acgcgctggg gatcaccagc atcgtggtgt cccatgatct ggcggaaacc
    23221 gcgagtattg ccgactacat ctatatagtc ggcgataccc aggtgctggg gcagggtacg
    23281 ccagccgagt tgatggcgtc ggatgatccg cgcgtgcgtc aatttatgca gggcatcccc
    23341 gacggcccgg tgccgtttca ctttcctgcg gccaattacc gcgacgatct tctgggggag
    23401 cgctgatgcg caagacatct gtgctcgagc gggtgcgcct gttcggtcgc gccggtattg
    23461 atgtagtcgc cacgctgggg cgttcggggc tgtttctgct gcgcgcgctg tttggccgag
    23521 gctcgacggg caacagcttt cagctactga ttaagcaact gtattcggtt ggggtgatgt
    23581 ccctggccat catcgtcgtg tctggcatat ttatcggcat ggtgctgtcg ctgcagggct
    23641 ttagcattct ggccaaatac ggctcggaac aggcagtggg gcagatggtc gccctgacct
    23701 tgctgcgtga actggggccg gtggtcactg ccttgctatt cgccgggcgt gctggttcgg
    23761 cgctgaccgc agaaatcggc aatatgaaat ccaccgagca gctatcgagt ctggcgatga
    23821 ttggcgttga cccgctcaaa tatattgtcg cgccgcgctt gtgggccggt tttatctcga
    23881 tgccgctgct ggcgatgatt ttcagcgtgg taggtatctg gggcggtgcg atggtcgccg
    23941 tcgattggtt gggggtttat gaaggctcgt tctgggccaa catgcaaagc agtgtttcgt
    24001 ttcgtgaaga tgtgctcaat ggtgtgatca agagcgtggt ctttgcgttt gtcgtgacct
    24061 ggatcgccgt ttttcagggc tatgactgtg agccgacttc ggaagggatt agtcgtgcca
    24121 ccacgaaaac cgtggtgtac gcctcgctcg ccgtattggg gctggacttt attctgaccg
    24181 ctttgatgtt tggagatttc tgatgcagaa ccgcacgctg gaaattggtg tcggcttgtt
    24241 cctgttggcc gggttgctgg ctttgctgct gctggcctta cgcgtcagcg gcctgaccgt
    24301 cggcagcagc gacaatacct acaaggtgta tgcgcacttt gaaaatattg ccggtttgac
    24361 ggtcagatcg aaagtgacca tggccggcgt aacgatcggc aaggtcaccg cgattgatct
    24421 ggaccgcaac agctatatgg ggcgcgtcac catggtgctg gatgatgccg tggataacct
    24481 gccggcagac tccaccgctt cgattttgac ggcgggcctg ctgggcgaga aatacattgg
    24541 gcttagcgtg ggtggcgaag aagaagtgct gcgcgatggc ggcaccattc acgacacgca
    24601 gtcgtcattg gtattggaag acttgattgg caagtttttg ctcaattcgg tcaacaaaga
    24661 ccaggctaat taatagaggt gttgagcatg atcaaagcat tgcgtaacgg tctttttatc
    24721 tgtctggcgg ctatgtcctt gccattgctg gcggctcctg cggcccatga ggtggtgcag
    24781 cagaccacca ccacgctgct ggctgacctc aaggccaaca aggaacagta ccgcactgat
    24841 ccgggcgcgt tttataccgc cctgaacaac attctcggcc cggttgtgga tgtcgacgga
    24901 atctcccgtg gcgtgatgac cgtgcgttac tcgcgccagg cctcgccgga gcagatgcag
    24961 cgctttcagg aaaacttcaa gcgcagcctg atgcagttct atggcaatgc cctgctcgaa
    25021 tacaacaatc aggacatccg tgtgctgccg gtcagtggcc agcaggaccc tgagcgcacc
    25081 tcggtaaata tggaaatcaa ggacggtaag ggcgtggtct atccgttgtc ctacaccatg
    25141 gtcaaccaag gcggcacctg gaagatgcgc aatgtagtta tcaacggtat caacgtcggc
    25201 aagctgttcc gtgatcagtt cgcccaggcc atgcaggaca atcgcaatga cctcgacaag
    25261 gttatcgaca actggaccga gaccgttgcc aaggcccgcc agggggcgaa tgcatcgtga
    25321 gtcaggccag catccgcgag gcgtcgcctg gggtttttaa cctgactggt gtgctcgatt
    25381 acctgagcgc cccggcgctg cgcgagcaag gcgcgagcct gctcaaggtc agtccggcgg
    25441 ctgcttgcgt gctcgattgc agcggcgtgg tgaaatccag cagcgtcggg ctggcgctgt
    25501 tgctggcgtt tatgcgtgac gccagtgcgg cgggcaagac cctgagcgtg agcaatctgc
    25561 ccaaggatat gcgtgagata gctggcgtct gtggtctgct tgaaattatt ccgctgcagg
    25621 cctgagcagg gcttcggtcg gctaggaata atgcggggtt cgcaggcggg aggctttttt
    25681 gtatgatgcc aggcccgcga gcgtctgctc ggcagtcgtt aatttatgca gtgatcgagg
    25741 ttgagcatgc aggccttaga agtaaagagt ctccttgagg ctaaattgcc ggatacccag
    25801 gtggaagttg aaggcgaagg ctgcaacttt cagctcaacc tgatcagtga cgagttggcc
    25861 gcccttagtc cggtcaagcg ccagcagcag atctacgcgc acctcaatgc ctggattgcc
    25921 gatggcagca tccacgccgt gagtatgaaa ttctttagcc gtgccgattg ggccgcgcgc
    25981 tcctaaaccg agagaatcat ggacaaactg attattaccg gcggtgtgcg ccttgatggc
    26041 gagatccgca tttccggggc gaagaactct gccctgccga ttctcgccgc caccctgctc
    26101 ggtgacgcgc ctgtcaccat ctgcaacctg ccgcacctac acgacatcac caccatgatc
    26161 gagctgttcg gtcgcatggg tatcgaaccg atcattgacg agaagctcag cgtcgaagtt
    26221 gacgcgcggg caatcaaaac cctggttgcg cactatgagc tggtgaaaac catgcgcgct
    26281 tcgatcctgg tgctcggccc gatggtcgcg cgctttggtt atgccgaagt ggccctgcca
    26341 ggcggctgcg ccatcggttc gcgtccggtt gacctgcaca ttcgtggcct cgaagccatg
    26401 ggcgcgatca tcgatgtcga agccggttac atcaaggcca aggccccgga aggcggtctg
    26461 cgtggcgcgc acttcttctt tgataccgtc agtgtgaccg gtactgaaaa catcatgatg
    26521 gccgcgactc tggccaaagg ccgcagcgtg ctggaaaacg ccgcgcgcga gccggaagtg
    26581 gtcgacctgg ccaactgcct gatcgccatg ggcgcgaaaa tcagcggcgc cggcactgac
    26641 accatcacca tcgatggcgt tgagcgcctg cacggcgcgc gcttcagcgt gatgcctgac
    26701 cgtatcgaaa ccggtactta tctggtggct gctgctgtta cgggcggccg ggtcaaggtc
    26761 aaggacaccg atccgaccac cctggaagcg gtgctgtcca agctgcagga agccggtgcg
    26821 gaaatcacct gtggcaacga ctggatcgag ctgaacatgc acggcaagcg gccgaaagcc
    26881 gtaaacctgc gtactgctcc gtacccggcg ttccccaccg atatgcaggc gcagtttatt
    26941 gccctcaatg cgattgccga gggcaccggc acggtgatcg aaaccgtgtt cgaaaaccgc
    27001 tttatgcacg tgcatgaaat gatccgcatg ggcgcgcaga tccaggtcga aggcaacacc
    27061 gccatcgtca ctggcgttga aacgctcaag ggcgcgccag tcatggccac cgacctgcgt
    27121 gcgtcggcca gcctggtaat ttcggcgctg gttgccgagg gcgacaccct gatcgatcgc
    27181 atttaccaca tcgaccgtgg ttacgagtgc atcgaagaaa aactgcagat gctgggtgcc
    27241 aaaatccgcc gtattccggg ttgatgccat gctgactatt gccctttcca aaggccgtat
    27301 tctcgatgac accctgccgc tgctggcggc ggcgggcatc gagccgaccg agaacccgga
    27361 caaaagccgc aagctgatca tccccaccac gctggacgat gtgcgcctgc tgatcgtgcg
    27421 tgccaccgac gtgccgacct atgtcgaaca cggcgcggca gatctcggcg tggccggcaa
    27481 ggacgtgctg atggaatatg gcggccaggg cctgtacgag ccgctggacc tgcgtattgc
    27541 ccagtgcaag ctgatgactg ccggtgccgt cggcgcgccc gagccgaaag gccggctgcg
    27601 ggtggccacc aagttcgtca acgtggccaa gcgttactac gccgagcagg gccgtcaggt
    27661 cgatatcatc aagctgtatg gctccatgga gctggcaccg ctggtggggt tggctgacaa
    27721 aatcattgac gtggtcgaca ccggcaacac cctgcgcgcc aacggtctgg aagcccagga
    27781 actgatcgcc atgatcagct cgcggctgat cgtcaacaag gcgtcgatga agatgcagca
    27841 cgcacgcatc caagcgttga tcgacaccct gcgcgaagcg gtcgaagcgc gacatccacg
    27901 ctgagtctga tggccgcccc gtgcggccta gcctatccgc gttatagcca aaattctcag
    27961 gtgcccgcgc ggagtggttg ttactctacg ggcgcttgag tatttgccaa tctaagaggc
    28021 ccgctatgac cgctcctatc gctatccgcc gactcaatgc cgctgaccag gactttgccc
    28081 agcatctgga tcatctgctg agctgggaaa gtgtctcgga tgatgcggtc aaccatcgcg
    28141 tgctggacat catcaaagcc gtgcgtgagc gtggtgatgc ggccctggtc gagttcacca
    28201 agcagttcga cggcctggat gtcgcctcca tggcggacct gatcctgccg cgcgagcgtc
    28261 tggagctggc cctgacccgc atcacgccgg agcagcgcag cgctctggag aaagccgccg
    28321 agcgcgtgcg cagctaccac gaacggcaga agcaggattc ctggacctac accgaagccg
    28381 acggcacggt gctggggcag aaggtcacgc cgctggatcg cgccggcctg tatgtgcctg
    28441 gcggcaaagc ctcgtaccca tcctcggtgc tgatgaacgc catcccggcc aaggtcgctg
    28501 gcgtgccgga agtggtgatt gttgtgccga ccccgcgtgg cgaaatcaat gagctggtgc
    28561 tggctgccgc ctgcatcgcc ggcgtcgatc gggtgttcac catcggtggc gcccaggccg
    28621 tggccgcatt ggcctatggc accgagagcg tgccaccagt ggataaaatc gtcggccccg
    28681 gcaatatcta tgttgccacc gccaagcgcc atgtattcgg caaggtcggt atcgacatga
    28741 tcgccggccc ttcggagatt ctggtggtgt gtgacggcca gaccgatccg gactggatcg
    28801 ccatggacct gttctcccag gctgagcacg acgaagacgc ccagtcgatc ctagtcagcc
    28861 cggatgccgc cttccttgat cgcgtcgccg agagcatcgc caagctgctg ccgactctag
    28921 agcgcgagac tattgcccgc acttcgcttg aggggcgcgg cgcgctgatt ctggtagccg
    28981 atatggccca ggccatcgaa gtggccaacc gcattgcccc ggagcacctg gagctgtcgg
    29041 tggaaaaccc tgaagagtac ctgccgctga tccgtcatgc cggcgcgatc tttatgggcc
    29101 gctacaccgc cgaggccctg ggtgactact gcgccggccc caaccacgtg ctgccgactt
    29161 ccggcaccgc gcgctattcc tcgccgctgg gggtgtacga cttccagaag cgctcgtcga
    29221 tcatccattg ttcggccgag ggtgcgtcgg agctgggcaa ggtcgccagc gtgctggccc
    29281 gtggcgagtc gctgaccgcc cacgcccgca gcgccgaata ccgcatcaag aactgaattt
    29341 tcagggcgcg ccccgcgcgc ctcacccaag atcaaattcg aggagagaag ggcagatgag
    29401 caaattctgg agccccttcg tcaaagacct ggtgccttac gtgccgggtg agcagccgaa
    29461 actggccaag ctggtcaagc tcaacaccaa tgaaaacccc tatggcccgt cgcccaaggc
    29521 gatcgccgcc atgcaggctg agcttaacga caacctgcgc ctgtacccgg accccaatag
    29581 cgaccgtttg aagcaagcgg tggctgacta ttacggcgtg cagaccaccc aggtgtttgt
    29641 cggcaacggt tccgacgagg tgctggcgca cgctttccac ggcctgttcc agcacggcca
    29701 accgctgctg tttccggata tcagctacag cttctacccg gtgtattgcg gcctgtatgg
    29761 catcgacttc gtgcaggtgc cgctggatga gcagttccag attcgcgtgg aagattacgc
    29821 gcggcccaac ggcggcatta ttttccccaa cccgaacgca ccgactggct gccctctggc
    29881 gctggatgcc atcgagcgca tgctcaaggc caacccggac accgtggtgc tggtcgatga
    29941 ggcctatatt gatttcggcg gcgaaacggc ggtcagcctg gtggataaat accccaacct
    30001 gttggttagc cagacgttat ccaagtcgcg ctcactggcc gggttgcggg tcggtatcgc
    30061 ggttggccat cctgatctga tcgaagcgct ggagcggatc aagaacagct tcaactccta
    30121 cccgctggac cgcatggcga ttgccggtgc ggcggcggcg ttcgaggaca aggtctactt
    30181 cgagcagact tgccggcagg tgattgccag tcgcgaggcg gtggttgccg ggctggataa
    30241 actgggtttt gaggtgctgc catcggcggc gaacttcgtc ttcgcccgcc atccgggcaa
    30301 ggatgccgcg accctggcgg ccggcttgcg cgagcagggc gtgatcgtgc gtcacttcaa
    30361 gcaggcgcgt attgcccagt tcctgcgcat taccatcggc acgccggagc agaaccaggc
    30421 gctgctggat gccctgcaaa gtctgtaaat tgcagccata aaaaagccgg agcagtgctc
    30481 cggctttttg ttatctgtga gacgcgctga ccggcttcgc caacgttttt caacggactg
    30541 cgaaccccag gcgggctgtc agtccgctca gctaccattg acgggcggtc ttacgccgac
    30601 ttctgcggtc agttgtagcg gcttgccgtt gcgcaggata tcgatgttga tcttctgtcc
    30661 cggcttggcg cgcgctacct ggttcatcga gcggcggcca tcaccggcgg cctcaccgtc
    30721 gatgctgagg atcaagtcgc caggctgcag gcccgctttc tgcgctgggc cgtcgcggta
    30781 gatgccggca acaacgatgc cggggcgacc gtccatgccg aaggattccg ccagctctgg
    30841 agtcaacggc tgcacctcga tgcccagcca gccgcgaatc acctggccgt gctcgataat
    30901 cgcctgcatt acatccactg ccagttttac tgggatggca aagccgatgc cctgggagcc
    30961 gccggatttg gagaaaatcg cggtattgat gcccaccaga ttgccgtagg catccaccag
    31021 cgcgccgccg gagttgccgg ggttgattgc tgcgtcggtc tggataaagt cttcgtaggt
    31081 attgaggccc agctggttac gcccggtggc gctgatgatg cccatggtca cggtctggcc
    31141 aacgccaaag gggttgccaa tggccagggt cacatcgcca atgcggatat tgtccgagcg
    31201 gccgaggatg ataaagggca ggtcctgcag gtcgatcttc agcaccgcca ggtcggtttc
    31261 cgggtcgctg ccaatcagtc gcgccagggt ttcgcggcca tctttcagcg ccacgacgat
    31321 ctgatcggca ccggcaacca catggttatt ggtcagcagg taaccttccg ggctcatgat
    31381 cacggccgag cccaggctcg actccatgcg acgctgctgg ggcaggttgc caccgaagaa
    31441 cttgcgaaac tgcgggtcct cgatcagcgg atgcgcaggc tttttgacga attttgtggt
    31501 gtacaggttg gccacagcgg gcgatgcggt ggttaccgct tcggcatagg acaccggacc
    31561 ttcctgcatg cggctataga aagctgcctg gcgcacatgc acatcctgtc ctggcaagcc
    31621 gacccattgc ggaaaatact gcatgatcag cagtgccgac agcacaccga ccaacagggg
    31681 ccagccgagg aaacgcaggg ccttgaacat cgatccaatc ctgagggttg ccgggggcgg
    31741 gtgtgcccct taaggtgcgc cattatagag aagccgctga gaataaagca ggcctcgcaa
    31801 ctgttgattg ataaggaaac cctatggcca ttgccctggc gaccctggtg gaagaagccg
    31861 accgctacct gaacgccgcg cgcatcagcg attactgccc caatggcctg caggtcgaag
    31921 gccggccgca ggtacggcgt atcgtcagtg gggtgaccgc tagccaggcg ctggtcgatg
    31981 ctgccgttga ggccgaggcc gatgtactgc tggtgcatca cggttacttc tggaagggtg
    32041 aaaacccctg catcacagga atgaagcgcc gccgtctgca aaccctgttg gccaacgaca
    32101 tcagcttgct ggcctatcac ctaccgctgg atgtgcatgc cgaggtcggc aataacgtgc
    32161 aactggccgc gcagctgggt attacggtgg aggggccgtt ggagccggag agttcgcgta
    32221 ccgtcgggtt gctcggctcg ttggccgagg ccatgagtcc gcgtgatttc gcccatcatg
    32281 tgcatcaggt gctcggtcgt gagccgctgc tggtcgaagg caatgagatg attcgtcgag
    32341 tcggctggtg caccggtggc ggccagggtt atatcgacca agcgattgcc gccggggttg
    32401 atctgtacct gaccggtgaa gcctccgagc agacctttca cagcgctcgg gaaaacggcg
    32461 tcagctttat cgctgccggc catcacgcca ccgagcgtta tggcgtgcag gcgctgggcg
    32521 attatctggc gcggcgcttt gccattgagc acctgtttat tgattgccca aacccaatct
    32581 gaggcgcaat tgcggccttt cctgcaggta taaagctaga tcgtttcgat ctaactgggc
    32641 tcctgaatag aagggggcgc tgtgctagag tgcgccctcg tccacggccc gccggccaaa
    32701 acacaccgaa tttcgtgagt agccatgctc gataaactga cccacctgaa acagctggag
    32761 gcggaaagta tccacatcat tcgtgaagtg gccgccgaat tcgacaaccc ggtaatgctc
    32821 tattccatcg gtaaagattc cgccgtgatg ctgcacctgg cgcgcaaggc gttcttcccc
    32881 ggcaagctgc cgtttccggt gatgcacgtc gatacgcgct ggaagtttca gtagatgtac
    32941 agcttccgcg agaagatggt taacgagtat ggccttgacc tgattaccca caccaatcct
    33001 gatggcgtgg cgcaggacat gaaccccttc acttacggca gtgccaagca caccgacgtg
    33061 atgaagactg agggtctcaa acaggcgctg gacaaatacg gcttcgatgc cgccttcggt
    33121 ggcgcgcgcc gcgatgaaga gaaatcccgc gccaaagagc gcgtgtactc gttccgcgac
    33181 agcaagcatc gctgggaccc gaagaaccag cgtcccgagc tgtggaatgt gtacaacggc
    33241 aacgtcaaga agggcgagtc gatccgtgta ttcccgctgt ccaactggac tgagctggat
    33301 atctggcagt acatctatct ggaaagtatc ccgattgtgc cgctgtattt cgccgaagag
    33361 cgcgaagtga tcgagatgaa tggcgcgctg atcatgatcg acgacgaacg catcctcgaa
    33421 catctcaccc ctgagcaaaa agccagtatt cataaaaaga tggtgcgctt ccgtaccctg
    33481 ggttgctacc cactgactgg cgcggtggaa tccacagcca gcaccttgcc ggaaattatt
    33541 caggaaatgc tgttggccaa aacctctgag cgccaaggcc gagttatcga ccatgactcc
    33601 tccggctcga tggaagagaa aaaacgtcag gggtactttt aatatgtcgc accaatccga
    33661 attgatcagc gaggacatcc tcgcctatct ggcccagcac gagcgcaaag agctgctgcg
    33721 ctttctgacc tgcggcaacg ttgatgacgg caaaagcact ctgattggcc gcctgttgca
    33781 tgactccaag atgatctacg aagaccatct ggatgccatc accaaagatt cgaaaaaagt
    33841 cggcaccacc ggtgaagata tcgatttggc gttgctggtc gatggcctgc aggccgagcg
    33901 cgagcaaggc atcaccatcg atgtggcgta ccgctatttc agcaccacca agcgcaagtt
    33961 cattattgcc gacacccccg gccatgagca gtacacccgc aacatggcca ctggtgcgtc
    34021 caactgcgac ctggcgatta ttctgattga cgcgcgttat ggtgtgcaga cccagaccaa
    34081 gcgccacagc tttatcgcca gtctgctggg tatcaagcac atcgttgtgg ccatcaacaa
    34141 gatggacctt aagggtttcg atcaaggcgt attcgagcag atcaaggccg attacctgaa
    34201 gttcgccgaa ggtattgcgc tcaagccaag ctcgctgcac tttgtgccaa tgtcggcgct
    34261 caagggcgac aacgtggtca acaagagcga gcagtcgcct tggtacaccg gccagtcgct
    34321 gatggaaatt ctcgagaccg tggaagtggc gggcgatcgc aacttcaccg acctgcgttt
    34381 cccggtgcag tacgtcaatc gtccgaacct gaacttccgt ggtttcgccg gcaccctggc
    34441 cagcggcatc gtgcgcaagg gtgacgaagt tatcgctctg ccatcgggca agggcagcaa
    34501 ggtcaaatcc atcgtcacct tcgagggtga gctggaacag gcaggtccag gccaggcgat
    34561 taccatcacc ctggaagacg aaatcgacgt ctcccgtggc gacatgctgg tgcacggcga
    34621 caaccgtccg caggtggtcg atagcttcga cgcaatgctg gtatggatgg cggaagagcc
    34681 gatgctgccg ggcaagaaat acgacatcaa acgcgccacc agctatgtgc cgggctcgat
    34741 tgccagcatc actcaccgga ttgatgtgaa taccctggaa gagggcgccg cgagcagcct
    34801 gcagctcaac gaaatcggcc aggtgcgcat cgccttggat gcaccgatcg cgctggatgg
    34861 ctatacacat aaccgcacca ccggctcgtt tatcgtcatt gaccgcctga ccaatggcac
    34921 cgtcggcgcc ggcatgatca tcgccgaacc gcttggtgcc cagggctcgg acggccatca
    34981 cggcaagctg gcccatgtgt ccaccgaaga gcgtgctgcg cgcttcggcc agcagccagc
    35041 caccgtgctg ttcagcggct tgtctggcgc tggcaaaagc actctggctt atgccgtcga
    35101 gcgcaagctg ttcgactccg gccgtgcggt ctacgtgctg gatggccaga acctgcgtca
    35161 tgacctgaac aaggggctgc cacaggaccg cgccgggcgc accgagaact ggcgtcgtgc
    35221 cgctcacgtg gcgcgtcagt tcaacgaagc cggcttgctg accctggctg ccttcgttgc
    35281 gccggatgcc gaaggccgtg agcaggccaa ggcgctgatc ggtgccgagc gtctgattac
    35341 cgtgtatgta caggcttcac cgcaagtctg cgccgagcgt gatccgcaag gcttgtacgc
    35401 ggccggtggc gacaatattc cgggtgagtc cttcccttac gacatcccgc tgaatgccga
    35461 tctggtgatc gatacgcagt cggtgtcggt ggaagagggt gtcaaacagg tgttggctct
    35521 gctgcgcgag cgtggcgcga tctaaatccg ctgcacagta aaaagccccg cctagtgcgg
    35581 ggctttttat tggctgcttg gtcgccgaag cgaccgagtc agcggctatt acttattctt
    35641 caaaccgtag ctttcatcga gcatgcccgg cacattggcg cttttgggcg cgtagtcctt
    35701 gggcatttcg ttgtttctcg gtggcgtcag gcgttcgcgt ttttcgctgg catcgtcggc
    35761 gtgcagggtg gccagcaggc gttgccgggt cagctcgtcg agggccaggc ggttggcgcc
    35821 gtcggacagg tgttcctgta catcctgata gctctgggta agctttttca ccatgctggc
    35881 ggtggtgttg aagtggctga ccacctcact ctgataagcg tcaaagcgct cctgcatttc
    35941 atccagctgg cgctgggtgc ggctaggggc ggcattgggt gccaggcggg caatcaggaa
    36001 gccaatggca atgccggcaa ccagggtaat ggccggtagc aaccaggcgg tgagcgtctg
    36061 ttccacgagt ccttcctctc taaacggctt tgctttacgt taacggctcg aacctgcgct
    36121 gtataccgca atgcaatgcc gaaacgtttg cggtggtgtg tgcggcgcag gctgtcagct
    36181 agacgagtcg acccactgcg aggtcacgga gttccctgtt gctcacccgc gaaacccctc
    36241 tgatgattgc cggccccagc ggccaactcg aagccctgtg gctggatacc cccgacgtcc
    36301 gtggcgtggc gctgatctgc caccctaatc cggtgcaggg cggcaccatg ctcaacaagg
    36361 tggtctccac cctgcaacgt actgcccgtg acagtggcct ggccacgttg cgctttaatt
    36421 accgcggtgt cggcgccagt gccggcagcc acgatatggc cagtggtgag gtggacgatg
    36481 ccgaggccgt ggccaactgg ctgcgtgcgc agcatcctga attaccgctg accttgctcg
    36541 gtttttcctt cggtggtttt gtcgccgcag ccctcggcgc gcgcctggaa gcccagggcg
    36601 taacgctcga caagctgttt atggtggccc cggcggtgca tcgcttgagc gaagaaacgc
    36661 ccccggccag taactgcccg ctgatcctga tccagcccga ggacgatgaa gtgatcgacc
    36721 cacaggtggt ttaccagtgg tcggcggcac tcgggcgtgc ccatgagctg ctgaaagtgg
    36781 cagaatgcgg ccactttttt cacggcaagc tgacggatct aaaggatctg ctgctgccgc
    36841 gtctctgaac tgattgagcc ccaataagcg aacgttatga ccactcgtat cattaccggc
    36901 atcaccacca ccggcacgcc gcacctgggc aactacgcgg gcgcgattcg cccggccatc
    36961 gttgccagcc gcgctgccga tgcggattcg ttctacttcc ttgctgacta ccacgcgctg
    37021 atcaagtgcg acgagcccgc gcgcatccag cgttcgcgtc tggaaatcgc tgcagcctgg
    37081 ctggcgttgg gcctggatac cgacaaggcg actttctacc gccagtcgga tatcccagag
    37141 atccccgagc tgtgctggct gctcacctgc gtcgccggca agggcctgct taatcgcgct
    37201 cacgcttaca aggcctcggt ggacaagaac gttgagcagg gcgaagaccc ggatgcgggc
    37261 atcaccatgg gcctgttcag ctacccggtg ctgatggcgg cggacatcct gatgttcaac
    37321 gcgcacaagg tgccggtcgg ccgtgatcag atccagcacg tggagatggc ccgcgatatc
    37381 ggccagcgct tcaaccacct attcggcaac ggccgtgagc tgtttaccct gcccgaagcg
    37441 gtgatcgaag aagacgtggc gactctgcca ggcctcgacg gtcgcaagat gagcaagagc
    37501 tacgacaaca ccatcccgtt gttcggcagc gccaagcagc tcaaggatac cgttgcacgc
    37561 gtcgtgaccg actccaaatt gccgggcgag ccaaaagacc cggacaactc gcacctgttt
    37621 accctctatc aggcttttgc tgcgccgacc cagcaggcag atttccgcgc tgcgctggag
    37681 ggcggtatgg cctggggcga agccaagcag gcgctgtgca acctactgga agccgagttg
    37741 ggcgaagcgc gcgagcgtta ccacgcgctg atcagcaaac cggctgactt ggaagacatc
    37801 ctcctggccg gtgccaccaa ggcgcgcaag accgccacgc cgtttctcgg tgagctgcgt
    37861 gaggccgtgg gcctgcgctc cttccgcagc caggccgtca acacggcggg tgaaaagaag
    37921 aaagtcggca agagcgcacg ctttgtcagc ttccgtgatg atgacggcag cttccgcttc
    37981 cgcctgctgg atgctgctgg cgagcaattg ctgctgtcca agtcgtttgt cgacggtaaa
    38041 gcggcaggca tggtcaacaa gcgtctgcaa tccggtgaag cattggatgt gcgcgagcag
    38101 gacggcggtt ttggcgtctg gctggacgat gagtgcgtgg cacaaagccc ggtatttgcc
    38161 gacagcagtg cttgtcaggc ggcgattcag cgcctgcgtg aggctctagc acctgcagag
    38221 taacgcgtat gacggtgcat taatcggatt tccgcctgcc agccgtgctg gccgattgcc
    38281 aatcaacggg ggcgtcgcta aagtgacgcc cccgttttac ttgcccggct aacgaattta
    38341 tgacgcccct agaacgcacc aggctgacct taaacgccct gatttctttc atgacgctgc
    38401 ccaggaaact gcggtgcgtc atctgcagcg cctgtatgac gatctggtcg ccgcagacaa
    38461 ggggacgact ggcgtgttcg gcaagctgtt cgccagaaag ccccaggggc cggtcaaggg
    38521 cctgtacttc tggggcggcg tcggccgtgg caagacctat ctggtcgaca ctttcttcga
    38581 ttcgctgccg ttcgagcgca aggtgcgcac gcacttccac cgctttatga agcgcgtgca
    38641 cgaagaaatg cgcaccctca agggcgaaaa gaacccgctg accattatcg gcaagcgctt
    38701 cgctgacgag gcgcgggtga tctgcttcga cgaattcttc gtcagtgata ttaccgatgc
    38761 aatgatcctc gccaccctgc tggaggagct gttcaagaac ggcgtcagcc tggtcgcgac
    38821 ctccaatatc gtgcccgacg gcctgtacaa ggacggcttg cagcgtgcgc gcttcctgcc
    38881 ggcgattgcc ctgctcaagc agcacaccga gatcgtcaac gtcgacagcg gcatcgacta
    38941 ccgcctgcgc gccctggagc aggccgagct gttccactgg ccgctggatg ctgccgccga
    39001 ggaaagcctg cagaagagct ttgtcagcct gctgcccgag cactgcgtcg tgcaggaaaa
    39061 cgagccgctg atgatcgaga accgcgccat cgttgcgcgc aaggtcggtg atgacgttgc
    39121 ctggttcgac ttccgcgagc tgtgcgatgg tccgcgtagc cagaacgact acatcgaact
    39181 gggcaagatc ttccacgccg tactgctggc caacgtcgag cagatgagca ccgccaagga
    39241 cgatatggcg cggcgcttta tcaacctggt ggatgagttc tacgaccgca acgtcaagct
    39301 gatcatctcc gccgaagtgg agctgaaaga cctctacacc ggcggtcgcc tgaccttcga
    39361 gttccagcgc accctcagcc gcctgctgga aatgcagtcg catgagtttc tcgcgcgccc
    39421 gcacaagcct taactcaaca cgcttacgct cgtacccaaa gcccggatca atccgggctt
    39481 tctagttgat ggcccactga agagatccgg gccgtctgca cccaggcagg ataaactctg
    39541 cgccatcgac ttttcgttat ctgcggttag ccctatgact tttgcctccc tcggcctgat
    39601 cgagcctctg ctgcgcaccc tcgacagcct cgactacaaa actcccactg ccgtgcaggc
    39661 ccaggccatc gtgccggtcc tgcaaggccg cgacctgatg gcggcagcgc agaccggcac
    39721 cggcaagacc gcaggctttg ctctgccggt gctgcagcgg ctgatgatgg aaggcccaga
    39781 agtttccagc aactcagtgc gtgcgttggt actggtgccg acccgagagc tggccgagca
    39841 ggtgctgcag agctttatca cctacggcca gcacctgccg ctgcgcagtt acgcggtgta
    39901 tggtggggtc agcatcaacc cgcagatgat gaagctgcgt aaaggcgtgg acgtgctggt
    39961 ggccaccccg ggtcgcttgc tcgacctgtt ccggcagaac gcggtgaagt tcaatcaggt
    40021 gcaaaccctg gtgctggatg aagccgaccg catgctcgac ctgggttttg ccaacgagtt
    40081 ggaaagtgtg ttccgcgcac tgccgaaaaa gcgccagacc ctgttgttct ccgcgacctt
    40141 ctccgaagcg attcgcagca tggccggcga aatgcttaat gatccgctga gcatcgaggt
    40201 cagcccgcgc aacgctgccg ccaagtcggt caagcagtgg ctgatcccgg tcgacaagaa
    40261 gcgcaagagc gagctgtttc tgcatctgta tcaggccaag cgctggagcc aggccctggt
    40321 ctttgtgaaa acccgcaagg gcgttgacga gctggaagcc gagatgcagc gccatggcat
    40381 cagcgccgac tcgatccacg gcgataaacc ccaggccacc cgtcagcgcg ccctgcagcg
    40441 gttcaaggat ggcgaggtca aggtgctggt ggccaccgat gtggcggccc gtggcctgga
    40501 tatcgatgat atgccgctgg tggtcaactt cgacctaccc atcgtcgccg aagactatgt
    40561 gcaccgcatc ggccgcaccg gtcgcgccgg ctctacgggg caggccgtgt cgctggtctg
    40621 cgccgatgaa gtgcagctgc tctcggccat cgaaaccctg acccagcaag tgctgcaacg
    40681 cgtcgatgag ccggacttta tccccgacca ccgcgtaccg cagaccctgc ccggcggcca
    40741 ggtggtgaaa aagccgaaga aggccaagaa gccaaaagta gttggcggcg gcaaaggcgg
    40801 cagcctgggg cgctggatgg aggctgacga gccggccgcg ccgacggtca aggcggtgcg
    40861 caaggtgccg agctttggcg gtaagccggg caagcccagc agaaagccct aaaccgcaat
    40921 gttagcccgg agcaatccgg gctaaagccg cacgcctttc agtcaggcac ccgctgggtg
    40981 ttctgcgcaa tccgccaaag cacgccgctg gggtcgaaga tcacgaagtc gcgcatgccc
    41041 cagggctgat cctgcggtgg caccaagcgt acttcgaagc gctcgctgag ctgttcatcc
    41101 agcaccttct gccaccaggc atcgacgtct tccaccagca ggtgcatgac gaaattctcc
    41161 gcttgctcct tgacgtagaa gtcctgcagc agaaaggcgc aatggtcgcc gtggctgaaa
    41221 taggccaagt catcgccgac ccagcccggc ttgaagccca gcgcctgata gaacgcctga
    41281 ctcaaggcga aatcgcgggc cggtacgaag gttttcagtt caacgctttg caacattgga
    41341 tgcttcctgc acgaaagtga tcgagcaggc tgataacggt tgcgccggcc ctctgtcggg
    41401 gcctggcgaa gagagattaa cggctggcct gcgccgcttc cagctgcttt ttgtgggttg
    41461 ccgcagcggt catgtcgtag atcaccacgc agatatgctc gacatcgccc ttggccccgg
    41521 ccagcggcag cagggtgacg ttctggtaca taaagtcttc ctggccggtg atcggctggt
    41581 agttcttgaa gtgcatcagg taggggtgct gctcccacag gctgaacgcg cgggtgccca
    41641 attgcaccac ggtgtcgacc ttgcgccgta gccaggtttc gttgatctcc gggaacaggc
    41701 tgaacagggt tttgccgtgc acctcatccg ggcccatgcc ggagtggttt tccataaagc
    41761 tgttccacac ctcgacctga tattgacggt caagcacgat gacgccgaca tcgatgcact
    41821 gcacgatatc gagcagccag tgcacttctt ggatatctat ctgtgcgggc atggcttaac
    41881 tcatcaggta gttgattttg ttggtcaggc gcttgatcga gtcttcggta aacagcagca
    41941 gcaggtcgaa gtggatattg tgcgcctcca ggctgtagct gatttccacc gccagggtct
    42001 ttttccagcg gcgctgattg aggtgaatca gccgctcgat ggaggagtgt tggccgagca
    42061 attgcggatg gccctgggag aagcgcacgt cgatctgctc ggcaattccc gccaggcagg
    42121 cgccgatcag gatgctggcc aaatccagaa gcatttccga ggtttccaga tcgtccttcg
    42181 gctgccagtc gagcaggcgc gccatatcgg ctacttctga atcatggaac aacagcagcg
    42241 actcgccggc tatgccgtcg ccgataaagc cctggcacac cgcgctcagg cgttcgccgc
    42301 gcatggcatc ggtcagggtc atatgcagct cgctgacctc gaagatattc acctgcggca
    42361 ccggcaactg cacaaatacc ccgagcgcct tggccagcag cgcggcggcg cggcccatgg
    42421 cgacgttggt gacctcgcgt aaggcgtcgc ggaaattgac cttcagatca tcgagcaccg
    42481 ccgcctgggc gatggcgccg ggtgtggctt tggggtcaaa caagcctagt tcgagtaggg
    42541 tggcgcgcag tacctcgggg tcggcgggct tcttgataaa ggccagcgcg ccgagctcct
    42601 tgacccgccg tagcgcttcg tcctgcacgt caccggaaac cactactact ttgcaggcca
    42661 ggccttcggc gcgcagcgcg gctagggttt cgtagccatc cattaccggc atggtcaggt
    42721 ccagcagcat cacctggccc aacccctggc gcagcgcagc aatggcttcc tggccgttgg
    42781 tggcctggct gatggtcact ggccattcgg gcggtagggc gcggatcaac tgcttgcgcg
    42841 ccatgttcga gtcgtcgcag accactagag ggatcacggc tggagattac ctgtgcgcgt
    42901 tgaagagcac cgctctgggg cggtttcact caagcatagc cgtttgagat tgtgcgcgga
    42961 ggcttattcg gccgggtgtt gcagccattc cagtatgccc tgggcggcgc gtcggccgct
    43021 ggcaaagcag gcggtcagca gatagccgcc tgttggggct tcccagtcga gcatttcccc
    43081 ggcgcagaag gtaccgggca gaggcttgag catcagctgt gcgtcgagct gctcgaaggg
    43141 cacaccgcca gctgtgctga tcgcttcatc cagtggccgt gggcggatca gggtgatcgg
    43201 cagggacttg atcacgccgg ccagcaacgc gggatcattg aaggcagcag cgctggccag
    43261 ttcgcgcagc agcgtggcct tgaccccgtc gataccggcc tggcgatgca gatgtttggc
    43321 catcgactgc gagccgcgtg gtttgctcaa ggctgcctgc aacttggcca gcggcgtctg
    43381 tggcagcagg tcgaggtgca ctgtcgcgtt gccatgctgg ttgatcgttt cgcgaatatc
    43441 tgccgacagg gcatagacca ggctgccttc gataccactg gcagtcagca cacactcacc
    43501 ctgacgcggt gctgcaccag ccaggttaag gctgacgttc tttagcggcg cgccggcgaa
    43561 cttatcccgc agaaacgggc tccaggcgct gacatcgaag ccgcagttgc tcgcctgcag
    43621 gggcgagata gtcacgccac ggtcttgcaa caggctgacc caggcaccgt ccgagcccag
    43681 tcgcgcccaa ctgccgccgc ccagagccag cagggtggcg tcggtattta cggtgtgttc
    43741 gccggtggac gaggcgatac gcaacgcgcc atcgttagtc cagcccagcc agcgatgccg
    43801 ggtatgtatc tgtacgccca gttcgcgcaa ccgtttgagc caggcgcgca gcagcggtgc
    43861 ggctttcata tcggcgggga atacccggcc cgaggtgccg acaaaagtgt cgatgcctag
    43921 cccatgaatc caggcgcgca gggcgtcggc atcgaaatcc tgcagcagct ctgctacttc
    43981 cgactgacgc tggccgtagc gggcgacaaa agccggcttg gcctctgagt gggtgatatt
    44041 catccctccc accccggcca ggaggaactt gcgccccact gaaggcatac cgtcgtaaag
    44101 atcgacctgc acaccgccct gggcgagcac ttcagcggct atcagcccgg cagggccgcc
    44161 gccgataatg gcaacgcgtg gagctggctt tggcaaggca ctgttcatgg cggtttcggc
    44221 gtggggtgtg gccgcgcatt ctaaccgagg cggcgtccgc cttgtcattt gccctggctt
    44281 cgggcagggt cgctggagac ttttctgcct ggagcccctt atgtctgacc tctaccccag
    44341 catcgacccc gacggcctga tcgaatactc ggtggtctac accgaccgct ccctcaacca
    44401 tatgtcgcag tcgtttcagg gcgtgatgaa ggatatttcc agcaccctga aacaggtcta
    44461 taacgcacat gcgatggcca tcgtgcccgg tagcggcacc tatggcatgg aagcggtggc
    44521 gcgtcagctg gccaccggac agaagtgcct ggtgctgcgc aatggctggt tcagctaccg
    44581 ctggacgcag attttcgaga tgggccagat cccctcggag tcgattgtgc tcaaggcccg
    44641 cccgatggcc gaaggcagcc aggcggcatt ctcgccgcca cggctggtcg atgtgctggc
    44701 ggtgatcgct gccgaaaaac cgcaggtggt gttcgccccg catgtggaaa cctcttccgg
    44761 gatgattctg ccggacggct atctgcgcgc tgtggcggat gccgtgcatg cggtcggcgg
    44821 cctgtttgtg ctcgactgca tcgcctccgg caccctctgg gtggatatgc aggcctgcgg
    44881 tatcgacgtg ctgatcagtg ccccgcaaaa aggctggagc gcttcgccgt gctgcgccct
    44941 ggtcatgttg agtgaagcgg cccaggcccg cgtcgaagcc actcaaagca ccagctttgc
    45001 ctgcgacctg aagaaatggc tgcagatcat gcaggcctac gagcagggcg gccacgccta
    45061 ccacgccacc atgcccagcg atgccctggc gcgtttccgc gatgccatgc gcgaggccga
    45121 agccatcggc tttgccaccg tgcgtaacca gcagcaggaa ctcggtgatc gcgtgcgcgc
    45181 attgctggcc agcaagggct ttaaaagcgt cgccgccgaa ggcttcgaag caccgggtgt
    45241 ggtggtttgc tacaccaccg acgccagcat caagaacggc agcaaattcg ccgcccaagg
    45301 cctgcagatc gccgctggcg taccgctgca gtgtgatgaa ccagcggatt tccagacctt
    45361 ccgcctaggc ctgttcgggc tggataagct gggcaatatc gaccgcaccg tggcgactct
    45421 ggaacaggcg ctggataagg tgttggcggg gtaggtcgag tcgtaggatg ggtcgggccg
    45481 cgcaggttga gcgacgcgat acccatcatt ctatcggcgt tgcagcgata cctgccgatg
    45541 ggtatcgcta cgctcaacgc catcctacgg gctgttctgt gcttattgct cggacgcatt
    45601 cgtggcctta tccagcgcgg tcactatggc gtgcgcggcc ttgattctgg cggtgttggg
    45661 gtagttcttg ttggccagca tgaccaggcc gaggttctgg ctggggacaa acacggcgta
    45721 ggcgccgaag ccgccggtgg agccggtttt attgaacagg ctgtcgcctg gctcggtttc
    45781 cggcggtgcg aggcgtttag caggttgcga ctctagggcc atggccggcg agttgccggc
    45841 ttgcaggcgt tccaggctaa tcgggtaggg gtaacgctcc cagccgagac cctgggtcat
    45901 ctcgccgacg cggtaatagc ccgtgtgggt aatggcgatg gcgcgctgta ggtcttcgct
    45961 caggcctgca gaattcagat tggcctggac aaaacgcagc atgtccttcg cattggtttt
    46021 caccccgtag gcttcggcgt cgagcatccc aggcgtgacc cgcagcggct ggttgtcctt
    46081 gctgtagcca taggcatagc gcggcatctg cgccgctggc acctggatat agctgtgcgg
    46141 catgccgagt gcggggaaca gcgtgccctc catcagcgtg gggaacggct ggcccatact
    46201 cttggcagcg agcatgccca gcaggccgat gctggggttg gaatagcgtc tgtgagtacc
    46261 ggcgatatgt tcgggctgcc actggcgata ataggcgagc atgctggccg ggtcggtcac
    46321 tctgtcgggg aactgcagcg gcagcccgcc ggcggtgtag gtgccgaggt tgagcaggct
    46381 ggcgtgttcc aactggctgc cgcgcagctc gggccagtgc tggccggcgc tgtcggcgag
    46441 ggtcagcttg ccggttgtct gcgccagggc ggcgagggtg gcggtgagcg gtttactgag
    46501 tgagccgatt tcaaacaggg tgtcgctggt caccgcttgc tggtcatcca gacgcgccac
    46561 gccatagttg aagtagtgtg cctggccgtt gtgagtgatc gccacggcca tgccggcgat
    46621 atcgtattcc tccattaccg ggcggacggt tgcgtccacc agcgattgca gcctgtctgt
    46681 tgcttgcgcg acagtgcagg tagcaagtgc gagtgcggtc agcagtgttt tgagtcttcc
    46741 gtgcatggtg tgcgagattc cttgatcgtg aagatgcgcg atatgaccgc gcccggtgct
    46801 ggttttatcc cggccctgtg ccgttgatgc gttgcctgac tgggcaggcc agaacccgtc
    46861 ttgcagagac tgcaacaggt agggtgcgct gtgcgcacca aggatttcaa accgggttgg
    46921 tgcgcacggc gcaccctacg agaccaggct atctccggct cagtggtgat ggtcgaatgc
    46981 aatgctctag taggcaatac gttatcccaa cccgtgcgcc tgccatacct ttgccgcgtt
    47041 gtgatgcagt atgccgtggc gccgggccag ggccatgcgg tccttgtcgt agccgccgcc
    47101 gatcagcccc accacgggta tatcgcggtc caggcagtgg ttgagcacgg ctgcgtcgcg
    47161 ggcagcgatg ccggcgtccg tcaggttgag gtagccgagg gcgtcgtgct ggtgcacgtc
    47221 gacgccggcg tcgtagagca cgatatccgg ctggtacagc gccagcaggt agttgaggca
    47281 gtcatccacc accttcaggt actcggcatc acccatgccg ttgggcaggg ggatatccca
    47341 gtcgctggcg gccttgcgcg ccgggtagtt cttctcgcag tgcagtgaaa cggtgatcgc
    47401 ctccggggtg ttttccagca ggcgcgctgt gccatcgccc tggtgcacgt cacagtcgaa
    47461 aatcagcaca cgctgggcct tgccgctggc cagtaggtac tgactgatca ccgctaggtc
    47521 attgaagatg cagaaaccgg ccggatgatc gtaatgcgca tggtgggtgc cgccggctag
    47581 atggcaagcc aggccgtgct gcagggccag ctccgccgcg agcagcgaac cgcccacggc
    47641 gcggatggtg cgctgcgcca gggcggagct ccagggcagg ccgaggcggc gttgctcttc
    47701 atggggtagt tcgccgctgg caaagcgctg gatataggcc gggcagtggg tcagggcgag
    47761 tacatccgtc gggcacagtt cagggcgcag cagctcggcg tcggtgctta ggccgctggc
    47821 gacgatgtga tcgcgcagca ggcgaaactt ctccatcggg aagcgatggt tggccgggaa
    47881 aggcggactg taatcgtcgt ggtagaccag cggcaggcgc atggtggcgg gctcgggcgg
    47941 aagtgaccga agtatggcgg caggtttatc cgacactcaa gctcggctgt cgattggcaa
    48001 taatgctgcc attattgtcc gcatccatct ttcagtcggg ccgacaagcg ttggcaaagt
    48061 ctttaccatc tcgcccccag ttaggtgcgt ttacaggaga gcatgcatga gtcaggtgtt
    48121 gaatgagttg gtcgccttgc tgaccctgga aaccatcgaa gagaatctat tccgtggcgt
    48181 cagtcaggac ctcggctttc gccagttgta tggcggccag gtgctgggcc agtgcgtctc
    48241 ggcggccagc cagacggtgg ataccgcgcg acatgtgcat tccatgcacg gctacttcct
    48301 gcgccctggc gatgccacgc tgccggtggt gtatcaggtt gagcgcgtgc gtgatggcgg
    48361 cagcttcagc acgcgacggg tgacggcgat ccagaagggt aagccgatct ttacctgcag
    48421 cgcgtcgttc cagtctgatg aagaaggctt tcatcaccag aacctgatgc cagaagtgcc
    48481 aggaccggaa gagctgaagt cggaaaccga gctggcgcgc ctggtggtcg atgccttgcc
    48541 ggagcgcatg cgtgagcggg cggtgagcga caaaccaata gaaatccgcc cggtaaccgt
    48601 cggcaacccc tttgcgccaa agcccagcga gccggtcaaa catgtgtggt ttcgcgccgc
    48661 tggcgagttg ccggatgatc cgcagctgca caaatatctg ctgggctacg ccagcgactt
    48721 caatttgctg acgacttcga tgctgccgca cggcgtttcc gtttggcaga agtttatgca
    48781 ggtcgccagc ctcgaccact cgctgtggtt tcacggcaac ctgcgtatgg acgactggct
    48841 gctgtacagc atggacagcc cctgggccgg caatgcccgt ggcttctccc gtggcagcat
    48901 cttcaaccgt cagggccaac tggtggcttc cgttgcgcaa gaggggctta cccgcgtgcg
    48961 tgaggattgg aagtgagtct gagcgccgcc cgccattggg tgttcgatat ggatggcacc
    49021 ctgactctgg cggtgcacga cttcgaggca atcaagcggg cgctggatat tccgctggaa
    49081 gacgacatcc tcggtcatct ggccgcattg cctaccgctg tagctgcggc caaacacgct
    49141 tggctgctgg agcatgagcg cgaactggcg ttgaccgccg cgccggcgcc aggggcaatc
    49201 gagctggtac gcgcgcttca cgggcgtggc tgccgcctcg gcatcctcac ccgtaatgcc
    49261 catgagctgg cgctgctgac tctgcgcgcc attggcctgg atgactgctt tgccgtgccc
    49321 gacgtgatcg gccgcgatga agcgccgccc aagcccgatc cgggcggcct gctgcatttc
    49381 gctagaacct ggcaggtggc accgagcgag ctggtgatgg ttggtgatta ccgctttgat
    49441 ctggaatgcg cccgcgccgc cggggcgcgc agcgtgctgg ttaatctggc ggaaaacccc
    49501 tggccggaat tggccgatca tttcgccgtg gattgtcggg cgttgcagcg ctcgttgtag
    49561 ggtgcgctgt gcgcaccgcc tggtaatcgt tgtggtgcgc gcagcgcacc ctacggggtg
    49621 attggggcgt tgtggttgca gcaaccagac tccaaatcct tgaggatcgg gcagtctggg
    49681 cggtcgttgc cctggcagtt gtccatcaaa tccctgagcg tgtcgcgcag gccgctgagt
    49741 tcctcgatct tgcgttccag ttcgctgata tgtgcggcgg ccagggcttt gacatcggcg
    49801 ctggcgcgtt ggcggtcctg ccacagagcc agtagctggc tgacttcggc cagggagaag
    49861 cccagatcgc gcgagcgttt gataaacgcc aggcgatgca gatcctgctg gctgtactgt
    49921 cggtagccgc tgtcggtgcg gccggcggcg ggcagcagat cgatgctttc gtagtagcgg
    49981 atcatcttgg cgctaaggcc ggttttcttg gcggcttggc cgatattcat ggttgcctcc
    50041 caatttgcgg tttggcagtg aaaatgtgat cgcgggcatg gcccgctcct acgggtttat
    50101 cggttcgtcg tgtaggagcg gcggggacgc ccagtcccat ggccgcgaat agggttaaga
    50161 gcgatccttg ggcttccagg ttttcagcag caaggcattg ctcaccacgc taacgcttga
    50221 cagcgccatg gctgcgcccg cgagcatcgg gttgagcagg ccggcggcgg ccaggggaat
    50281 gccgaccagg ttataggcaa aggcccagaa caggttctgg cgaatcttgt tgtaggtgcg
    50341 ccggctgata tccagcgcat cggccaccag gcgcgggtcg ccgcgcatca gggtgatgcc
    50401 ggcggcgtgc atggccacat cggtgccgct gcccatggcg atgccgacat cggcggcagc
    50461 cagtgccggc gcatcgttga tgccgtcgcc gaccatagcc accactgcgt ttttcttcag
    50521 ctcgctgatg atcgccgcct tgtccgccgg cagcacttcg gcatgcgcgg cgctgatgcc
    50581 cagggctgcg gccaccgctt tgacgctgcc ggggttgtcg ccgctgatca ggtggctggc
    50641 gatgccttga gcggtcagtg ctgcgatggc ctcggctgcg cctggtttaa gtgtgtcgcc
    50701 aaaggcaaac agcccgagca cctgcggttg cggtgctaac tccagcagcc aggacagggt
    50761 gcggccttcg gcctcccagg ctagagcgtc ggttgccagt gcatcgtggt gcaaaaagtg
    50821 ttcttccagc agtcgcttgt tgcctagtgc cagttgccgt tggccgatct gcccggcgat
    50881 accgcgcccg gccagtgcct tgctgttgct gacttcggcg aggctcagtt gctgttctgt
    50941 gcactgatcc aacaccgctt tggccagcgg atgttcgctg ccgcgctgca gggcgccggc
    51001 cagttgcagc agctgggtgc tatgttcggt gtcagggcca tcaaccgcgc gcagatgggc
    51061 gatgcgtggc gcaccggaag tcagggtgcc ggttttatcg aaagccacca gggtcaccga
    51121 atgggcgact tccagtgctt cggcgtcctt gatcaaaata ccgtggcgcg ccgccacgcc
    51181 ggtaccggcc atgattgccg tgggcgtggc caagccaagg gcgcagggac aggcgatcac
    51241 cagcactgca acggcattta gcagcgcggt ttcgatacct gcgccaagca gcagccagcc
    51301 gagcaaagtg gccagcgcga tcagcagcac gctgggcacg aagacctggc tgactttatc
    51361 caccagcttc tggatcggcg ctttcgccgc ctgggcgtct tcgaccaggc gaataatccg
    51421 tgacagcacg gtttccgcgc ctagggcggt ggtttccacc agcaggcggc cttcaccgtt
    51481 gattgccccg gccgtgacgc tgtcacccgg ttgcttgggc tgcggcaggc tctcgccgct
    51541 gatcagcgct tcgtcggcat ggctctggcc ttcgcggacc aggccatcga ccgggaagcg
    51601 ttcgccgggc ttgaccacga tggtgtcgcc cagttgcagc tcacccagcg ccacccattg
    51661 ctcgacgccg tcctgcaggc gcagagcgcg ttccgggcgc aaggcctgca gggcacggat
    51721 ggcgctgctg gtctggcgtt tggcgcggct ttccaggtat ttaccgagca gaatcagggt
    51781 gatgatcacc gccgaggctt cgaaatacag gtgcggcatg ctgccggctg gactcatcgc
    51841 ccattgatac aggctcaggc cgtaggcggc gctggtgccg atggccacca actgatccat
    51901 attcccggca cgggcgcgca gggcgttcca ggcggcgcgg tagaagcgtg cgccgaagac
    51961 gaactgcacc ggcgtggcca gggcgaactg cagccaggcc ggcagcatcc agtgcaggcc
    52021 gaagggctcg acgagcatgg gaatgaccag cggtacggtc agcacaatcg ccagcagcag
    52081 ggcccagcgc tcgcgctgca gatgccgctc ggccgttggc tgagcctggg ttgcatcgtc
    52141 cagcacgctg gcgcggtagc cggcttggct gacggcctgc agtaaagcat tggcatcagt
    52201 gccacgcagc acctgcaact gcgcccgctc gctggccagg ttgacgctga ccgagagcac
    52261 gctcggttgc tgcgtcaggg cgtgttcgat acgccccgcg cagctggcgc aggtcatgcc
    52321 ggaaagcgct agttccaggc gctcgctggg cacctcataa ccggccgcgc taacggcatt
    52381 gaccagttgt tccaggctgt ctgcgggcgc ttcgacacgc gcctgttcgc ttgccaggtt
    52441 gatgctgaca ctggcgacat cggtcacctt ggccagggcg cgctcgatcc gtccggcgca
    52501 gctggtgcag gtcatgccgc tgataggcag gttgaaggtg gtgaggctgg acatgggtgg
    52561 tcctccattg agttccagca caggatcaac cttgccatgc tggcaaggtc aagacctttt
    52621 atcccttggc cttaccctta tggcaaggcc aagactgcaa ttgtccggtt accatggcgg
    52681 cttatttatt caggaggttg tttatgcagg tttctcaggt tcacgcattc cgggtcgatg
    52741 gcatgacttg ccagaattgc gtcaagacca tcaccgaggc gctgttggct tgtgacccgg
    52801 cggcggatgt aaaagtgaag tttcccggtg gtgaagtacg ggtgagcagc agcgtgccgg
    52861 tcgatcaact gatggtggca atcgtcgagc agggctacgg cgtcagcctg gcgtaagcac
    52921 tgcagcgtct ggccagtcgc ggatcagccc gcgaaagcag gcatcgatca tcggcgcggt
    52981 atcgctgagc gggtcgaaca gattggggtc gcgcgaccag tcgtgaaaca ggccgatcag
    53041 cagcgcatgc acgatgcgcg cggccaggcg cgggcttagc cccgggtaca ggcgcggggc
    53101 gacggccggg gcactgaact gctgctcgca gagcgcgata aacaggttga taaaggcttc
    53161 gtggcgctct tcggcctcgc gcagctcttc ggtgaattcg cagcggcgca gcaggatggt
    53221 gaagatgcgc ttcttctgcg gcgagcgggc gaggttctcg atggcctcga tacagagctc
    53281 acgcagggtc attagcgggt cgtgagccgg gcagccgcgc atgcgttcgg ccagttgttc
    53341 gggcggcaga cgcacctggc cgagcatggc gctgaacagg tcggccttgt tggcaaagtg
    53401 ccaatacacc gcgccgcgcg tcaccccggc gtgccgggcg atgtgctcca ggctggtgtg
    53461 tgccacgccc ttttccagaa acagcgtttc ggccgcctca agaatggcga cgcgggtttt
    53521 ctcggcttct tctttggttc gacgcatggc ggcaggcgta tttctggcta ggctgagcac
    53581 aaagtgtaat gaacttaagg gtaatgttct atttacaaac acttgtgtat gtaactagca
    53641 ttgccggtct caaacgttgc gccttgcgtt ttttcggaga cacccatgct tttttcccgt
    53701 cgtctgccgg ttactgccgg ttccttgctg ttggcttttt tggccgcccc gctgtttgcc
    53761 gccgatgctc cgccagcgcc cgaggtggtg gtggaaactg tcaaagccga ggcactgccg
    53821 ctggagcttg aatactcagc gcgtactgcg ggtttccgtg aagtgcaggt gcgcgcccaa
    53881 gtcagtggca ttctgcagga acgcacctac ctggaaggca gccaggtcaa gcagggccag
    53941 gtgatgttcc gcattgaccc gcgcacctac caggccgcgc tgagccgcgc caagggcgcg
    54001 ctggcgcagg agcaggcgcg ctatcggcag accgagcgtg acctcaagcg catccgcgaa
    54061 ttgcagaaaa agggctttgc cagtgagagc gagctggaca atgccatctc caatttcgag
    54121 cagagcaagg ccaatatcca ggccgccgag gccgaggtgc agtccaagca gattgacctc
    54181 gactacacca cggtaaaagc gccgatctcc ggtatcacca gtaaggaaac cgtttccgaa
    54241 ggcagtctga tggtggccgg tgatccaagt gccagcctgc tgagcaacat cacccagctg
    54301 gacccgatct acgtcaactt cgccgcgccg gattccgatg tggaaagcgt acgcagcggc
    54361 ctgcagaacg gcagcctggt gctgccggaa gacggcaaga tgagcgtgca gatcaagctc
    54421 ggcgacggca gcgtctaccc gctggaaggc aaggtggact ttaccgacag cctggtggat
    54481 cgcggcacag gtaccgtcag cgcgcgcgct gtggtgccta acccggatca gacgctgttg
    54541 ccgggtcagt tcgtgcgggt gcaggtcaaa ggcctgagca ttcccaatgc catgaccctg
    54601 ccagagcgcg cgattgctca aggcccgggc ggtacctttg tctacgtggt ggatgaaggt
    54661 ggtgtggcgc gcatgcgcca ggtcaccacc gggcataccg ccaaaggccg ctgggtgatt
    54721 gtctctggca tcagcgcagg tgaccgggtg atcgtcgagg gcctgccgaa ggtgcgcccg
    54781 gacagcccgg taaaagttgt cgcccctgcc gccagccaat cctaaggagc gttcccggtg
    54841 atttcacgct tctttatcga ccgcccggta tttgccgcgg tcatctcgat catcatcgtg
    54901 ctggctggcc tgatggccat gcgctcgttg ccgatcgccc agtacccgca gattcttcca
    54961 ccacaagtgt cggtcagcgc cagctacgcc ggtgccagcg cccaggtgat tgccgaaaca
    55021 gtggccgcgc cgcttgaaca gtcgatcaac ggcgtcgagg ggatgatcta ccagcagtcc
    55081 aactccggcg gcaacgccat gagcctgtcg gtgtacttcg aggtgggccg cgacccggat
    55141 caggccacca tagacgtcaa caaccgggtg caggccgccc tggccaagtt gccggaagaa
    55201 gtgcgccggc agggcgtcaa ggtgcagaag aagtcctcgg acattctcca ggtggtcacc
    55261 ctgtattccc cggatggctc gcgcgacccg gtgtttatca gcaactacgc gctgatcaac
    55321 gtgatcgacg aactcaagcg cctgccgggc gtgggtgatg tcagccagtt tggctcgaaa
    55381 gactattcca tgcgtatctg gctgcgtccg gacaagctgg cgcagtacaa cctgacgccg
    55441 accgatgtgg tcaattcgat ccgtgagcag aactcgcagt ttgccgctgg cagcttcggc
    55501 cagcagccgc tcaagcagga gcaggacttc acctacacgg tgaccactca gggccgtttc
    55561 accgacccga aagaatttga aaacgttatt ctgcgtaccg acgacactgg cgccagcctg
    55621 ctgctcaagg atgtcgcgcg gattgagctg ggggcgcagg actactcact gatgacctcg
    55681 ctcaacggcc agcagaacgc cgctttcggt gtgtacctgc agcccggcgc gaatgccctg
    55741 gacaccgccg aagcagtgag caatacgctg gcgcggctgt cgaagaactt ccccagtggc
    55801 atgacctaca aggtgccgta cgacaccacc aaattcgttc aggtatcgat caaggaggta
    55861 attcacacct tcttcgaggc cttgatcctg gtggtgctgg tggtgtttat cttcctgcag
    55921 aactggcgcg ctacgctgat cccggtgctg gcgattccgg tttcgctggt cggtactttc
    55981 gccggtatgt acatgctcgg cttctccatc aacctgctga ccctgttcgg catggtgctg
    56041 gccatcggca ttgtggtgga cgacgctatt gtggtgatcg aaaacgtcga gcgggtgatg
    56101 gccaccgaca agatcggccc gcgtgaagcg accatcaagg ccatggagga agtgaccggg
    56161 ccgatcatcg ccatcgtgct ggtgctctgc gcggtattcg tgccggtggg cttcctcggc
    56221 ggcctggccg gggagatgta taaacagttc gccatcacca ttgccgtgtc ggtggtgatc
    56281 tctggcatcg tcgctctgac cctgagcccg gcgctctgcg ccttgctgct caagcctggg
    56341 catcacgagc cggcggcgcc gttccgcgcg ttcaaccggg tcttcgacaa gttgaccagc
    56401 ggctacaccg ccggcgtgcg tttcttcctc aagcgttcgg cggtgggttt gctgctgttc
    56461 ggcgcgatga ttgcgctgat ggtggtgctg tttaaccgag tgcccagttc gctggtgccc
    56521 aacgaagacc agggctatgt gatcaacgcc tacttcctgc cgccggccgc ctcgttgacc
    56581 cgtaccgaga agctgaccgg cgacgtgacc cagcagttga tggcgcaccc tgcggtgcag
    56641 gatgtggtga ccttcgccgg cttcgacgtg ctgacatttg gcacccgcag caacgccggg
    56701 gtgtccttcg tgccgttgaa agactggagc gagcgcacta cgccggagct ggatgcgcgc
    56761 aacctgaccg gcgaatttat gggcatgggc gctgcgcaga aagatggtgt agtgctgagc
    56821 tttaacccgc caccgatcac cggcatgagc accaccggtg gtttcgaagg ctttatccag
    56881 gatcgcagcg gcggcaccac cgcgcagctg gccgagaagg tccaagcctt cctcgccgct
    56941 gcggcgaaac gccctgagct ggctggagtg cagagcacct ttagcgccaa cgtgccgcag
    57001 tactttattg atctggaccg gaccaaggca cgtgccctgg gcgtggcagt gaatgatgta
    57061 ttcactgcca tgcaggccac gttcggtagc tattacgtca acgatttcag tctgtatgga
    57121 cgtaccttcc aggtcagtct gcaggccgaa gcggagttcc gcagcaagcc ggaagaccta
    57181 tcccaggtct atgtgcgcgc aggtagtggc gagctagtgt cgctggccag cctggtcaag
    57241 gttgagcgga ttctcggccc ggacacctac gcacgcttca acgtctaccc ggcggcgaag
    57301 atcctcggcg ggccggcacc tggcttcagc tcgggccagg cgctgagcgc gattcaggag
    57361 gtggcggatg aggtgctggg cagcgattac agcatcggct ggaccggctc ggccttccag
    57421 gaagtggcgt cgcaaggctc gggcagcagt gcctttatct tcggcctgat catggtgttc
    57481 ctgatcctcg ccgcgcagta cgagcgctgg accctgccgc tggcggtggt gactgcggtg
    57541 ccgtttgcgg tgttcggcgc gattctcgcg gtgtggctgc gcggcattga gaacgacctg
    57601 tacttccagg tcggcctggt tactctgatc ggtttggcgg cgaagaacgc cattctgatc
    57661 gtcgagtttg ccgtgctttg cagacgtcag ggcatgggca ttttcgagtc ggcgctggag
    57721 gcttcgcgtc tgcgtttccg cccgatcatc atgacctcgc tggcgtttat tcttggtgtt
    57781 gtgccgctgg cgatcagctc gggcgcaggc tcggccagtc gtcactcgat cggtacaggg
    57841 gtgatcggcg ggatgatggc ggcgaccttc ctggcgatct tcctgatccc gatgttctac
    57901 ctgttggtgg aatcgctggc agagaaaatc ggcaaaggcc ggaaaaaggc cgacacgccg
    57961 gcttgagcta ctgcccctgc tagcgggggc aagccgtagc ccggggcttg cccgggctac
    58021 aacggctcca tctgttatta cctctctgga aacccatgcc gttacgtctt ctgttgattc
    58081 ttggcgcgct cagcgccttt ggccctttgg ccatcgattt ctatctgccg agcttcccga
    58141 ccctggcccg cgcgttctct accgatgtgg agcatgtgca gctgtcgttg gccgcctact
    58201 ttgccggcct ggccattggt cagttagcgt acggtccgct ggccgaccgt tttggccggc
    58261 gcaaaccgct gctggtcggg gtcatgctgt tcagtctggc gtcgttggcc tgcgccctgg
    58321 cgccgagcct ggagtggctg atcgtcgcgc gctttgtgca ggccctgggt ggctgcgccg
    58381 gtatggtggt gtcgcgggcg gtggtgcgtg atctgtgtga cccgatcagc tcggccaagg
    58441 tgttttccca gctgatgctg gtgatgggcc tggcgccgat tctcgcgccg ctgggcggtg
    58501 ggctgctgct cagcacgttg ggctggccgt cgatctttat ctgcctgacg ctgttcagct
    58561 ttatctgcct gctggcggtg gctaaatggc tgccggaaac cctggccaag gacgcgccgc
    58621 cggcccccct gcgcggtgcg ctgggagagt acaagcggct gttcgctgac ttaccgtttc
    58681 tcggttatgc gctgactgga ggctttgcga ttgctgggat gtttgcctat atcgccggct
    58741 cgccgtttgt gtttatcgag ctgtatggca tccctgccga gcattacggc tggctgttcg
    58801 gcagtaatgc cctgggcttt attctggtgg cgcagttgaa tgcctggctg gtggcgcgac
    58861 atgggcctgc gtactggctg gggaaaaccg tgtggttcta cctggcgtgc ggcctggcac
    58921 tgctactggt ggcattggcc aagccgcaag ccttgtggcc actgctggtt ccattgttcg
    58981 gctgcattgc cagcctgggc attcttctgc ccaacgcctc agcctgcgcc atggccgggc
    59041 agggtagcca tgccggcagt gcctcggcgc tattcggtag tgtgcaattt gtcatcgccg
    59101 ccagtgccgc cgccctggtg ggcatgctgc atgacggcag cgcctggccg attgccgtgg
    59161 tgatcttcgc ttgcggggta ttggccgtgg gcttttccct gtttacccgt tgggtcgagc
    59221 gtggcgcgat ccgcgcataa ggtcgcagcc gttgaaacgc gtaggtggct ttcaacggcc
    59281 cgttagctgg cggcgcgctg ttgctcgaaa ggcagcctat gcggcgcgct gaggcgcgct
    59341 tgcagggtgc tgacaaactg gcgggcctcg ctttcactgc gaaaggtcac gctctgcttg
    59401 ccaaaaatta cttgccagcc cgcacctgcg gctgttttta acggttggat cattacgttc
    59461 atcgccgaac ctcctatgca agagaagcta gtttagtcgc gtaatgtgaa cgttcattga
    59521 cctgcaacaa atcaatcacg gctgacgtac cgttggtctg attcgcccac gttgggtgtg
    59581 ctgggggcgg ccctgaactg tacggccagc cccgccagcc gggtgaattc ggtgaccagg
    59641 gcgatatcgg ctgcgctcgg ccggctgggc tgcgggtagt agatgccgaa ggtgcccagc
    59701 acttgacaac tgtcatcctt gaacggcagt gaccagcaag cgtgcaggcc ggcggccagg
    59761 gcgacgctct ggtagtcgcg ccaataggga tgctggctga ggtcctcggc gattactaat
    59821 tcgccagttg ctgcggcatg cccgcatgag ccaacttcaa gtgctgcgcc aatcccctca
    59881 atcgcctggc aataagcctc cggcaggctt ggggcggcgc cgagatgcag gcgctggtgc
    59941 tcatcaagca gcatgatcga cacgcgcatc tgcggttgca ggctttccag gctgctgctg
    60001 atgccgttga gaatgctgtg cagcggttgt aggccgagca gctcgtcgag taccgcattg
    60061 cgcgcctgct gcagatgctg ggcttggtgg cgctcatgta cgtcatgcag gctgccgtgc
    60121 aggctcagct cgggtaggct caggttgagg tgcagttcaa ctcggcgcgc ctggccgcta
    60181 tgggtcagta gctggcactc caggcgcaaa ctatccagtt tgccgctgcg caggtcttcc
    60241 aggccttggc gcaacatggt ctgacccagc tccggtataa agcgctgtaa cgggttgccc
    60301 aggctttcgt ggatcgggta accgctgatc tgctcccagg ccgggttaag gaagctcagt
    60361 ttgtcgttgg catcggtaat aaaaacggca tcacccaggt gctcgaccag cgagcgatag
    60421 cggctttcgc tgatctgcag ctcgctgtcc attttcttct gttcggtcag gtcgatatcg
    60481 acacagtaca gctccatttg atcgaggctg ttgcgcatca tcaggtggct ggaatagacc
    60541 cacaccaggc tgccgtcttt gcgctgcaat tgcacctcgc cggcaggaat tggtgggccg
    60601 ccgagctgcc acagcttgat cgcgctggcc actttgctac gtgcctggag cggtacgatc
    60661 agctcttcca ggcggcgccc catcacttca tgcacggcat agccatagag caaggcgctg
    60721 gcctggttcc agtaaatcac tcggcgctcg cggtcgtagc cttgcacagc aatccgtggg
    60781 gtctgttcga acagtaggcg aaagcgttgt tcgctttcac gcagggcgct ttcatcgcgt
    60841 ttctgtgcgc tgatgttctg tacggtgccg aggatgcgcc cgtgctcgtc gacctcgccg
    60901 cgcagcatca gccaggtgtc ctggtggggc tggcgttgct gcaggcgggc gctcaccatc
    60961 atggcactgc ggccttccag caaggccagt agggcgcgtt gtacagcgga gcgctcagcg
    61021 gggtgcagcc agctgagcag ctgttcggtg ctgcactgct ggctgtcagg tgcgcggtcg
    61081 agcaggcgga gcgcctcttc actccaatgg aagccattaa ggtactccca gctgccgagc
    61141 ccagcgttgc gctgagtgtg ctgtagcagg cggctgttgt gggtgagttt ttccagcagg
    61201 tggcgttgcg cctggcggtc acgatggctg aacaggaaga tcagcccggt gctgagcaga
    61261 ataaacagca ggcctttgcc tgtttgcagc tcggccacca gctcgttgtc tttgaccagt
    61321 gcgtgcagca gataatcgct gccgaaaatc cacagagcac tgaacaggat atatacgcca
    61381 gccaggcgca gtgggccttg aagatcaggt cgcatccctt accgccaatg gctagccaag
    61441 tcgaaagaac tgagagggtg ttgcctgaaa agaggcaatg caatgagtct agctgtccgc
    61501 ttatcgaatg tagggtgcgc gataaaaaaa ctgcccggct cacgcgtaat gtgagccggg
    61561 gtgtttgcaa tgtctttaaa agccttcgag gacgatcttg cctttggccg tgccgctctc
    61621 cagcagagta tgggcgcggc gcaggttggc cgcgttgatg gtgccgaaat gttcgccgag
    61681 ggtggtgcgc agggtgccgg catccaccag ttcagccacc cgatccagca ggtgatgctg
    61741 ttcgatcatg tcggcggtct cgaacagcga gcgggtaaac atcagctccc aatgcagcga
    61801 caggcttttg cgcttgagct gcatgatgtc cagcggttgc tccgggtcat cgatcaacgc
    61861 caggcggcct tgtggagcca gtgcttcgac cagttgcgcg aagtgcttgt cggtctgggt
    61921 caggctggcg acgtgggtca cctgatggat gccgatgcgt tttagctctt cactcagcgg
    61981 caggctgtgg tcgatcacat gatgcgcacc cagctcactc acccaggcct gggtttccgg
    62041 gcgtgaggcg gtggcgatta cggtcaggcc ggtgagctga cgggccagct gcagcaggat
    62101 cgaaccgaca ccaccggcgg caccgacgat cagcaggctc tggcccaggt ctgcgttgcc
    62161 ccgagtgatc tgcaggcgct cgaacagcag ctcccaggcg gtgatcgcgg tcagcggcag
    62221 cgcggcagct tcggcaaacc ccagctttgc tggcatacgt ccgacgatgc gctcatccac
    62281 cacatgcagc tcgctgttgg ccccggcgcg gttgatcgcc ccggcgtaat acacccggtc
    62341 gccgggctgg aacaggctga cttcgctgcc taccgccttg acgatgccgc tggcatccca
    62401 gcccagcact ttggcggcac cgtcttccgg ggcgacgttg cgacgaatct tggtatccac
    62461 cgggttgacc gaaatagcct tgacctcgac cagcaggtcg cgcgggccgg gcgttggcgc
    62521 aggcagttcg acatcctgca gggcgtcggc gtggtcggtg ggtaggggct ggtaataggc
    62581 gacagctttc atgaagtgct ccttagagaa tacggtgcat cagttgatga tcgacctgtt
    62641 ccagcaggct gccgcaggtt tcgccgaagt gcagaaaatg tgcggtttgc tggtgcgcgc
    62701 taagggcggc ggcgtcctgc cattgctcaa tcatgatgaa gcggttgggc tgctcctcat
    62761 gcaggtgcag gttgtattgc aggcaaccag cttcggcctg gctcggcggc accaactggc
    62821 gcaggcctgc ttcaatcgcc tcggcatggc cggggcgcgc ggtgatggtg gcaatcaaag
    62881 tcaatggcgt ggtcatggcg gactccggtt caagtgctgc acatgctcgg ttatttgatt
    62941 gacgagaaaa accgcctgcc actagagtct ctttcaattt ttttttgata atgaatcgcc
    63001 atgctgcgtt ttgatgattt gcagttgttt gtcaccaccg ctgacctggg caacctttcg
    63061 gcggcggcgc ggcgcctgga tatttctccg gcggtggcca gtgccgggct taaacgcctg
    63121 gagcagcaac tgcaggttcg cctgttcagc cgctccactc gcagcctgcg gttgaccccg
    63181 gagggtgagc tgtttcttgg tcatgcgcgc ctggccttgc agagcctgga tgacggtcgg
    63241 cagcaattgg ccggcagcaa ggtcggcatc agcgggccgc tgcagctgtc cgcaccctcg
    63301 gactttggcc gtaacacctt gctgccctgg ctggatgaat ttcagcaggc gcacccgcag
    63361 gtgcagctgc gcctgctgct gggtgaccgg gtggccgatc tgtttcgcga accggtggat
    63421 attgccctgc gctatggcgc accggaggat tccagcctgg tcgccctgcc ggtggctgct
    63481 ctgaatcggc gcgtgttatg cgctgcgccg cagtacctgc acaagcatgg cgtgccgcag
    63541 tcggtggaag cgttggcggg gcacaactgc ttgctctata tgctcggcgg gcggctacat
    63601 gaccgctggc gtttcagcga tggcaaacgc gagcagggca tcagcgttaa gggtgaccgg
    63661 gtcagtgacg acgccgatgt ggtgcggcgc tgggcggtca gcggtgctgg gttggtgtac
    63721 aaatcctggc tggatgtggc cggcgatgtg cgcgccggac ggctccaggt gctgctacct
    63781 gagctgcgcg gcgagccgac accgctcaac ctgatctgcg cgcaccgcgc gcaactgagc
    63841 aagccggtgc tcttgctgcg tgagttcgtt caactccgct gcgcggagtt gttggcggat
    63901 gcgccgtggc cgaactaaga ttgccgccag cgcgctgcgg caatccatcg cagagcggct
    63961 ttgtcacatg cgccccggag caatgctaga gtcgcgcccc atgaaaatga cccgtactga
    64021 ccgtcgcttg ctggcctgga tgctgtattt cagcgttctg ttcagtgcgt tcgcctgcag
    64081 tatcggccat ggacagatgg ccggcctgca gctcagcggg ctgggcagcc agtactgctc
    64141 ctttgagggc aacttcggcg ccggtgctga tctggaaggt tccggtgtgg tagcgcccaa
    64201 tcctgctacc ggttccggtt gctccctgag ttctaccttc agtgcgatca tcctggcggc
    64261 gttcttcggt ctgctgggcc tgctggcgcc cagtcgcgca cccctgctga cgttcttctt
    64321 cattcctcga cctgtccgct atctctggcc ttcggccaat ccccgcgcct caccttcgac
    64381 tccatagcca tccggcttaa gccccggata ttgctgccgt tgcgtcgtac ggacttgccg
    64441 tgcgccaacg cttgtcgtga gctatcggag tcgacccatg agcaaaactg ctccctcttt
    64501 ttataacctg gcctggcgct ggcatttcta tgctggtctt tttgtgattc cgtttctgat
    64561 cctgctgtcg gtcaccggca tcatctacct gttcaagccg cagctggatc agctgatgta
    64621 tgccgacctg ctgcaggtaa cgcctgccga gcaggccctc tcggcggatc agcaactggc
    64681 cctggtgcgt caggcgtatc ccagggcgag cattagccag tacctgccgc cggtaaacgc
    64741 caagcgcagt gcgcagtttg tgctggaggc cgatggccag ggcctcaatg tgtttgttga
    64801 cccctacagc gccaggattc tcggtagcca ggatgcgcag ctcaatctgc aggccatcgc
    64861 tcgccagctg catggcgatc tgatggtcgg caccctgggc gatcgactga tcgaactggc
    64921 cgccggttgg ggcatcgtgt tggtggtctc cggtttgtac ctgtggtggc cgcgcgggcg
    64981 ctccggggcg ggcgtgctgt ggccaaggct gagtgctcgt gggcgtttgc tgtggcgtga
    65041 cttgcacgcg gtgacaggct tttggggctc gctgctgttg ctgtttatgc tgctcaccgg
    65101 catgacctgg accggcttct ggggcgcaca gtttgccggc gcctggaacc acttccctgt
    65161 agcgatgtgg gacgacgtgc cgcagtccgg caagctcacc ggcagcctca acaccagcag
    65221 tcagcagacc gtggcctggg cggtggaaac cacaccactg ccggcctccg atccacacgc
    65281 cgcgcataac ggccatgcca ctgccgaggc gccagccgcc ccggccaacc tgctgcagta
    65341 cgtggtcgat accgccgctg agcgcggcgt acagccgggt tacagcatca ccctgccgaa
    65401 aggtgaaacc ggggtgtaca gcatcgccat gtttgccgct gacccgcgta acgacgccac
    65461 tctgcacatc gatcagtaca gcggtgcggt gctcgccgac gtgcgctgga aggattacgg
    65521 cctggtggcg aaaaccgtgg aaaccggggt catgctgcat gaaggcaagt tgttcggctt
    65581 ggccaaccag ttgctgatgc tcgcggtctg cctgctggtg ctgctcagtg cgatcagcgg
    65641 cctgatcatg tggtggcagc gccgcccagc gggcaagttc ggcgtaccgc cgctgcgcca
    65701 cgacctgccg cgctggaaaa ccgcgattgt gatcatgggc ctgctgggcg cggccttccc
    65761 gctggtgggc gcatccctgc tgctgatctg ggcgctggat tggctgctgc tctcgcgcct
    65821 gcctgggcag cgtctggcca acggctaaat ggaataaccg gcgcgggctt ggcccgcgcc
    65881 aaggcttcgc gttacaacac ttgttatgga cagtcagtcc accacggagg aacaccatgt
    65941 tgttgaaaaa gaccctgctg attgccgctc tgctaagccc gagcctgttt gccagtgccc
    66001 acgaatacac cgctggtgag ctgcatatcg cacacccctg gtcgcgggaa atgccggccg
    66061 tagcgcccac cgctgctgct tactttgtgg tgcacaacaa gggcgcagag gctgatcgcc
    66121 tgctcagcgt cagcaccccg gtggccggca aggccgagct gcacgagcat attcacgccg
    66181 atggcttgat gaaaatgcag caggtgcaga acgtggctat tcccgctggc ggtgaagtga
    66241 agttcgagcc catgggctac cacgtcatgc tgttcaacct gaagcagcat gccaaggacg
    66301 gtgagcgctt tccgctgacc ctgaccttcg agaaggccgg tgcggttgag gtggaaattg
    66361 ccgtacacaa ggacgccccg gccggtgaga agacccatgg cgaagggcac gcgcattaat
    66421 ctgcgtaccc agccccgctg cgttgcagcg gggctgctgc tgttcggtga tgttcaccgt
    66481 cattgatttg catcagccgc cctgcttagg ccgttagtca tactcggcgc aggattcttg
    66541 agttgttgtg atgcctcctg atcgctcctt caccgcccat tatcgcgcga gctggatggc
    66601 ccttttggcc atgctgttgc tcaatctcgg gccgttgggg tcgcagctca gtcagcctgc
    66661 cgaaccggcc tggctgacgg aactggcctg tggggagcac gccggtggcc ataacagccc
    66721 gctggatcac gatgtgctgt gggccaagtg cggctattgc accctgttgc tcaacagccc
    66781 ggccaccagc tgtgccagcc cgaccctggc gcttgctgcc gttgtacctg ccgaccgaca
    66841 tatcgcgccc gtgcgcagcg gggttgtccg ctccgctatc ttccccggtt cgcgcagccg
    66901 agcaccacct gcctattcct gatcgccatt gccctgttgc ctgtgtcact accgcttagc
    66961 ggtgctggcc attccctggt tttgtctcga ttttggaata gttatgtcta ctcgttcgct
    67021 attgatcagt gctgcactgt ttcgtctggc tcctctctgt gccggtttgc ttctggctgg
    67081 tcaggggcat gccgcacaaa cggcagaaca catcgctgag ctgcagttgt cgccgttggt
    67141 aattactgcg gtacagcaac gctcaccact gactgtggtg gcctacccca aggacccgcg
    67201 ccagccggtg ccggccagtg acgccgccga ttacttgaag accattcccg gtttctccgc
    67261 gattcgcagc ggcggcagta acagtgatcc ggtgctgcgc ggcatgtttg gttcgcgcct
    67321 gctgctgctc accgacggcg ggcagatgct cggtgcctgc ccaggcagga tggacgcgcc
    67381 cagctcgtac atctctccgg aaacctttga cctgctaacc gtcaccaaag gcccgcaaac
    67441 ggtgatctgg ggccctggtg cctcggccgg ggtggtgcgt tttgaccgcg cgccggaaag
    67501 tttcggcgaa ctgggcgcgc gcctacatgc cagtgtgctg gctggctcca acgggcgttt
    67561 cgaccagacg ctcgatggcg ccgtcggcgg cgagcggggc tacctgcggg tgaccggcaa
    67621 ccgctcgcat tcggacgatt acgataacgg taacggcgac accatcccct cgcgctggga
    67681 caaatggaac agcgatgtgg ctctgggctg gacgccggat gccgacaccc tgatcgaact
    67741 caccgccggc cgtggcgatg gcgaggcgcg ctatggtggc cgtggcatgg acggcaccca
    67801 attcaagcgc gagagccttg gcctgcgcgt ggagcgcagc aacctcggtg gtgtgctgga
    67861 taaggtcgag gccaaggtct actacaacta cgccgaccac atcatggaca acttccgtct
    67921 gcgcatgccc gatccaacca gcatgatgcg caatcctatg gagtcgcagg ttgaccggcg
    67981 cacccttggc gcgcgtctgg ccgccacttg gaaatgggac gatctcgagc tggtcaccgg
    68041 gcttgatgcc cagcgcagcg agcaccgtgc gcgtcgctca acctaccgca tgatgggggg
    68101 cgtatttact ggtgctgacc gttacccgtg gaagaaagac gcgctgatgc acaactacgg
    68161 cctgttcgcc gaatccacct ggcagatggc tgaccgtgat cgactgatct ccggtgcgcg
    68221 tttggatcgc gcctcggcca aggatttgcg accgagcttc agcagcatga tgggcatgcc
    68281 gacgcccaac ccgacggcgg acgagacccg cgccgacacc ctgccgagcg gctttgtgcg
    68341 ttacgagcat gatctatctg gctcaccgac cactgtctat gccggtattg gccatgtgca
    68401 acgctttccc gattactggg agcttttctc cgccgggctc aatggccctg ctggtgcgga
    68461 caatgccttc gacggcatca agccggagaa gaccacccag ctggatatcg gtatccaata
    68521 caagggggag gcgctacagg cctgggcctc ggcctatgcc ggccaggtgc gtgactacat
    68581 cctgttcgac taccgcagta tgagcaccca ggccgacaac atcgacgcgc gcatcatggg
    68641 tggcgaactg ggtgtggcgt atgacctgag tgccaactgg aaaaccgatg ccaccctggc
    68701 ctacgcctgg ggcaaaaaca gctccgatgg tgaagccctg ccgcagatgc cgccgttgga
    68761 agcacgcctg ggcctgacct accagcgtga cgattggagc gtcggcggcc tgtggcgggt
    68821 ggtcgatggc cagggccgca ctgcggatgg gcgcggcaac gtcgtggggc aggactttgg
    68881 cgacagtgcc ggttttggtg tgctctcgct caacggcgcc tatcgcgtca gcaagcaggt
    68941 caagctcagc gcaggcgtgg ataacctgct cgacaagaac tacgccgagc acctcaacct
    69001 gggcggtgat gccggtttcg gcttcccggc tgacacccgg atcgacgagc cgggccgcac
    69061 cttgtgggcc aaggtcgatt tcgacttctg atcctgatag atctggcgtt ttggcaaagg
    69121 gctcatcatt tgatgggccc tttgcgtttc ctaatacagc cttaagcttg ccgcgcgaca
    69181 gtagtccccg cagacacaca acttcccctg catcacgagg atttcaccat gcgcaaactg
    69241 cttctgttgt ctctggtttt gaccagcccg ctgaccttcg ccgccaccca atgcaccacc
    69301 gccgagaagg cgcagtggca ggatcaggac aagttccagg ccgacctcaa agcccagggt
    69361 tacgagatca agaagttcaa ggtcaccggc ggcaactgct acgaaatcta cgggttcaac
    69421 aaagatggcc agaaggtcga aatctacttc gacccggtca gtggcaaggt catcaaaggc
    69481 ggcgttgaag gctgatgaac accgtcacca cacgcctctg ggacccgctg gtcaggttgt
    69541 tccactggtc tctggcgagc gctttcctgg ccaattactt ctttaccgaa gagggcgaga
    69601 actggcaccg ctggttcggt tactacgccg tggcctggtt ggcgattcgc ctggtgtggg
    69661 ggtttatcgg tacaccggca gcgcgctggg cggacttctg gccgacccgg gcgcgcctgg
    69721 ccgaacacct gcgggcattg ctcagtgggc gtgattacca ccgcatgggg cattccccgt
    69781 tgggcggtct ggtgatgatc ggcatgatgc tattgatgct cggccttggc gtgactggct
    69841 ttctgatgga ggaggtcgat tacttctggg gcgctgattt acccgaggac attcatgaat
    69901 tctgcgccaa cgcgctgatg gccctggtgg gcctgcacat cgcggcggcg ctgtttgaaa
    69961 gctaccgcct gaaggaaaac ctgccgctgt cgatgatcac cggcaagcgg cgcaagctgg
    70021 atcactgagt ttttatctgt cgcggctaaa gcccctccca caattcatac actccgtggg
    70081 aggggcttta gccgcgacgc ttttgcctaa accaggcgtg catccaggct gttttgcgcc
    70141 aggcgcttgg cttgctcctg ggtcatgccc aggctgtcat gcagggccgc gaagttctcg
    70201 gtgacgtagc cgccgaagta ggctgggtcg tccgagttca ccgtcacctt cacgccgcgt
    70261 tctagcatct gcaggatgtt gtgctggcgc atgtcgtcga acacgcagag cttggtattc
    70321 gacagcgggc agacggtcag cgggatctgc tcatcgataa tgcgctgcat cagccgctcg
    70381 tcttcgatgg cgcgcacgcc gtggtcgatg cgcctgatct tcagcaggtc cagggcttcc
    70441 cagatgtact cgggcgggcc ttcttcgccg gcgtgggcga cggtcagcag gccttcgctg
    70501 cgcgccttat cgaacgcgcg ctggaacttg cgtggcgggt ggcccatctc cgagctgtcc
    70561 aggccgacgg cgataaaggc atcgcgaaat ggcatggcct gttccagggt tttgaacgct
    70621 tcttcctcgg acagatggcg cagaaagctc aggatcaagc cgtggctgat gcccagttgc
    70681 ttctcgccat ccaccagcgc ctgtttgatg ccgcgcagca ccacttcaaa gggaataccc
    70741 cggtcggtgt gggtctgcgg gtcgaagaac ggttcggtgt ggatgacgtt ctgcgccttg
    70801 cacttgagca gataggccca ggtcaggtcg tagaagtcct gctcggtgcg caacacatcg
    70861 gcaccggcgt aatacaggtc gagaaactcc tgcagattgt tgaaggcata cgcactgcgc
    70921 agcgcttcga cgttgttcca gggcagggcg atcttgttgc gctcggccag ggcaaacagc
    70981 agctcgggct ccagcgagcc ttccaggtgc aggtgcagtt cggccttggg cagggcgttg
    71041 agccagtcgt acatggtcag atctcgttat caggcgtggt cgcgacaaag tttaaccgta
    71101 aggacaggat ttcgccgaat cggcctgcgt gcggatcgcg gatgattgtt cccacgctcc
    71161 gcgtgggaat gaggctacag atgctctgcg tctatggggc gcggagcgtc ccaggctggg
    71221 ctcccacgcc ggagcgtggg agccatccgt cggtttagct ggcctgctcc tcacgccgat
    71281 aagcatatgt atcggcaaag cgtgacatca ggtactcggc gctggtcact gacggatact
    71341 tgggtggatt ggcggcgtcc gagcaattgg gcaggcagct gatttcggtg tccgggtgcg
    71401 gctcggcgaa gaacggcatg gagtagcggt ccacccccag cgggctgatc acccggtgtg
    71461 gggtcgaggt gtaacggtcg ttgctccagc gcgcgagcat gtcgccgatg ttcaccacaa
    71521 agctgccggc aatcggcggt gcatcaatcc actggccgtc acggtggcgc acctgcaaac
    71581 cgcctgcgtc gtcctgatag agcagggtaa tgcagccgta atcggtgtgt tccccggcgc
    71641 cttgctgttc ggcactgctg gccgtgtggc gcggcgggta gtgaatcatc cgtagcacgc
    71701 tgatcggctc atgaaagcgt gtgtcgaaaa agtcgcggtt aatatccagg gcaatcgtca
    71761 tggcgcgcag cagggtttgc gccagcgcct gcatatcggc gtagtgctgt tccatcagcg
    71821 tcgcccagcc gggaaggtcg ggatggcgat tggtgccgcg cagcggtttg ccggccagca
    71881 cctcagggtg gttgaggtcc atatggaaac ccatgtcgaa ggtttccttg agatcgctgg
    71941 gcttgctcgg gtccagctgc tcggtggcaa tcgcgccgta gccacggtgg tgggcggtct
    72001 gggtgatatc gatcttgagc ttttctgcag ccggcagggc gaagaaagtc ttggcggcgc
    72061 tcagcagttc ggcgatacgc gccgcgctga ttgggtggcc ggtgatatag aaaaagcccc
    72121 agtcgcggca ggcgcggtcg atctgttcag ccacggcggg ccaggcggct tggtcgtcgc
    72181 tgtaaagtgg ggcgatatcg ataatcggta gggtgctcat agaggctccg ggtgggggca
    72241 tgctgcgagt cttctcgtac ccagggcagc gaaaaaggac gatgccggtt aaggcatcgt
    72301 ccttttgatg cggttctgcc agaaggcagg gacggcatct gggttatgac ttacttcggc
    72361 agttcagcat ttacgttttc cacgtagtag ttcatcgagg ccagttcggc gttggtggcg
    72421 gcgacgccgg ccgggatttt ctcgacgccg ctctggtcct tgatcgggcc ggtgaacggg
    72481 tggaaggcgc cgctcttgat gtcggcgatg atctgctcgg cttcggtttt cacctccgct
    72541 ggcaccaggt cgctgatcgg caggcgaatg gtgtcttcag ccaggccgcc ccagaaatcc
    72601 tgtggtgccc acttgccgtc gatcactgcc tgggtggatt tgacgtagtg cgggccccag
    72661 tcgttgacga tcgaggtgag cacggctttc ggcccgaagt gcgccatgtc cgaggcgtag
    72721 cccacggaat agacgccgcg gcgctcagcg gtctggattg gtgccgggct gtcggtgtgc
    72781 tggaacacca cgtccacgcc ctgatcgatc agcgcgttgg ccgcatcgga ttccttgccc
    72841 ggatcgaacc aggagttgac ccacaccacc ttgatttcgg tgccggggtt gtacttgtcc
    72901 agcgccagct ggatggcgtt gatgtcgcgg atcacttccg ggatcgggaa ggaggcgacg
    72961 tagccgatct tcttggtctt ggtcattttc gccgcgagga agccgccgac ataacgaccc
    73021 tcataggagc gcgacaggta ggtgccgagg ttcttgtcct gcttgtagcc ggtggcgtgc
    73081 tcgaaggtca gcttggggaa ttgcttggcc actttcagcg tcgggttcat atagccgaag
    73141 gaggtggtga agatcaggtc ataaccaccc ttggccatgt tgcgaatcac ccgctcggcg
    73201 tccgcacctt cagggacgtt ttccacgtag ctggtttcaa ccttgtcgcc gagctgtttg
    73261 atcaccgcct gacggccctg ctcatgctgg taagtccagc cgtggtcgcc aatcgggcca
    73321 atgtagacaa agccgacctt caatggatcg gcggcggaag cgcccaacgt ggcgctaaag
    73381 ccaagggcgg caacgagggt gcgcagcaga ggttttttgc tcttctcgaa catggtcggt
    73441 ggaactccga tgtgtgtggg tacgttgttg ttaaacagct gccggttgaa ttgcaaaaag
    73501 ctgaccaact ggacaattag cgcaccacgc cgcgctaaaa cgcagaaccc cgccggggcg
    73561 gggttctgtg gtggtgctgt gctggtgcgt aagagggttg gtgtgctgcc tgctggtgcg
    73621 gtattgctct tgtgatgtcg cggctaaagc ggagtgccgc ccagcccctc ccacagatca
    73681 ccacatattc tgtgggaggg gctttagccg cgactggcga tagtgcggtt caagcagcta
    73741 taacctctgc agcgaaaact atcaggaggc aacggaatgc cccattcaaa gcagctacgc
    73801 atcggccgtt tctccgagga tcagcgcatc tatctgctaa cggccactgt cgagacgcga
    73861 gagccgatct ttgcggattt cctgcttggc cggctgctgg ctgcagagct aaaagccgct
    73921 catttcgatg gtctggttga gtcactggcc tgggtggtga tgcctgatca tctgcactgg
    73981 ttgctggtgc tgcatcgcgg ctcactcagc gagttgatgc gtcgagtgaa aggtcgcagt
    74041 gctcggcaga tcaatccgct tgctggtcga agggggcaac tgtggcaaga cggttttcac
    74101 gaccatgctc tgcgccacga agaggatgtg ttgccggtgg cgcgctatat cgtggctaac
    74161 cccttgcggg cggggctggt ggagcgggtc ggtgattacc cgctgtggga tgcggtgtgg
    74221 ctgtaatggc tgtaaaagct gtcgcggcta aagcccctcc cacaagtcac cacgagcctg
    74281 tgggaggggt taggcggcac tccgctttag ccgcgaagaa gtaaaaactc agtgccccgg
    74341 ctgccagggc ttgccaaggg ataccggcgc aaacaggcga gtcttgatcg catcccgcga
    74401 gagcaatacc agcaccagaa tggtcgctac gtagggcagc atcgccagca ggttggacgg
    74461 gatcgacagg ccgataccct gcgccaccag atggatgatg ctggctaggc caaacaagta
    74521 ggccccgagg agcacgcgca atacccgcca gctggcaaac accaccagcg ccagggcgat
    74581 ccagccacgg ccagcggtca tgttttctgc ccacataggc gtgtaggcca gtgacagata
    74641 accgcccgcc aacccggcca tggcgccgcc gaacagtacc gcgagggtgc gtacgcgcag
    74701 taccggcagg cccatggcgc tggctgcgtc ggggttttcg ccgactgcct ggatgatcag
    74761 gccgatgcgg cttttcagca gcacccaggc caccagggcg aatagggcga aggacaggta
    74821 aaccagaatg tcctgggcaa acagcatgcg cccgatcagt ggaatctcgc tgagcagtgg
    74881 aatggcgatg ggctcaaaac ccgccagcgg tttgccgacc caggctgcgc caacaaaggt
    74941 ggacaggccg acgccgaaga tggtcagcgc caggccggtg gccacctgat tggcgttaaa
    75001 acccagggcc acggcggcga acagcatcga caacagcatg ccggcagcca tcgccagcag
    75061 cacgccgagc cacaggttgc cggtgcttag ggcaacgata aaaccgatca ccgcgccgaa
    75121 cagcatcatg ccttcctggc cgaggttgag cacgccgctc ttctcgcaga tcagttcacc
    75181 caaagccacc agcagcagcg gcgtaccggt gcgcaccatg gcgtaaaaaa tattgcttaa
    75241 caggtcgaga tccatcggtt gctccggatt tggctattgc tgcaattcat ctgcagaggc
    75301 ttccccctca ccccaaccct ctccccggag gggcgaggga gcggtactgc gctgacttga
    75361 agccgcgcgc cgtttctgtc ccctctccct ttttgggaga gggttatggt gagggggcgt
    75421 tcgttcaggc tgccgctttt ttcagtaccg gaaggctggc ccatttccac ttcagccgtg
    75481 gccgataaag gatcagcacg tcacaggcga gcagaaaaaa cagcatcatt ccctggaaca
    75541 gttgggtgat cgcctggggc aggttcatgg tcatctgtgc gttctcgccg cccaggtaga
    75601 gcagggccat cagcaggctg gcgaacagga tgccgaacgg gttgaggcgg ccaagaaagg
    75661 ccacggtgat cgccgcatag ccgtaaccgg gggagacctg cggcaccagc tgaccgatgg
    75721 ggccggccac ttcaccgaca cccgccagcc ctgccagccc gccgctgacc agcagggcaa
    75781 accacaccag acgcttctcg cgaaagccga caaaaccggc ggcgcggcga tcgaggccga
    75841 gcaccttgat ctgaaaaccc agaaagctct tgtgcagcaa cacccacacc gccaccaggg
    75901 ccagcagggc gaagtacagg ccggcatgca ggcgaccgtc ttcactcacc agcggcaggc
    75961 ggctggcatc accgaacatt gccgactcag ggaagttgaa gccggccggg tctttcagcg
    76021 ggccgtgcac gaagaacagc agcaggttca gcgcgatgta gttgagcatg atgctggtga
    76081 ggatttcgtt ggcattgaac tgggtgcgca gccacgcggt taggccaccc caggccgcgc
    76141 cggccgccgt gccggcgagc accacccaga ccagcgccca gcggctgtcc cagtcgatga
    76201 tctggatcgc catggcgctg ccggccaacg cgccgagcag cagctggcct tcggcaccga
    76261 tgttccagat gcgcgcctgg taggccacgg ccaggccaag ggcgcagagc agaatcggca
    76321 gcgccttgac cagcagttcg gagacgccat accagtcggc cagcggcgcg atcagcaggg
    76381 tgtgcagggt ttgcagcggg tcatggccga gcagggtgaa cagcagcgcg ccgctgcaca
    76441 gggtcagcac ggcggcgagc agtggcgata accagagcat ggcgcgtgat tgctggccgc
    76501 gcggttcgag ggataacagc atggaaaact ccgtcagctc atgttggcag caggggctgc
    76561 ctgcgtgctt gtgatagggg caaactcgcc ggccatccag cggccaacat caacggcttc
    76621 ggtctctgcc gtggcggcca ggggcgagag cttgccgctg cacagcgcgc cgatgcggtc
    76681 gctgatctgg aacaattcgt cgaggtcttc ggaaatcacc agaatcgccg cgccggcatc
    76741 gcgcagggca atcagcgcgc gatggatcgc cgccgctgca cccacgtcta cgccccaggt
    76801 cgggtgcgcg gccaccagca gtttcgggtt ctgcaggatt tcgcggccaa ggataaattt
    76861 ctgcaggttg ccgccagaca ggctgcgcgc ggcggcatcg gcatctggcg tttttaccgc
    76921 gaagcgtttg atgatctcgt cggccagggc gcgcactttg ccgcgctgaa tcaggccctt
    76981 gctcagcaaa ccctgctgga aggcggtgag catcgcgttg tcggccaggc tcatatccgg
    77041 cactgcgccg tggcccagac gctcggccgg gacaaaggcc aggcccagct tgcgccgcgc
    77101 atccgggtac agatcggcaa tcggctgtgt atccaggctg atgcgttcgc gctcgctgcg
    77161 cggcaggcgg gtttcgccgc taagcagtgc cagcagctcg tcctggccgt tgccggcgac
    77221 tccagcgatg ccgacgattt cgccactgcg cacctccagg cggatctggc tgaggtggca
    77281 accgaacggg tcggcgttgt gccagctaag gtcatccaca cgcagacgcg gcaggccgcc
    77341 aattactttc gggtagctgc tttccagacc ctcggcatcg ccgaccatca gccgggccag
    77401 ctgcaggtcg ctgcattcgg ccggcacgca atggccagat acccgtccgc cacgcagcac
    77461 agtggcgctc tggcacaagg cgcgtacttc gccgagcttg tggctgataa acagaatgct
    77521 gcacccttca gcagccaggc ggcgcagcgt aacgaatagc tcgtcggcct cctgcggggt
    77581 gagtaccgag gtcggctcgt cgaggatcag cagtttgatg tcctgcatca ggcagcgaac
    77641 gatctccacc cgctgccgct caccgatgga caggctgtgc accagccggc ccggttccag
    77701 ggccatgccg taacgctggg agacttcgcg gatcttcggt tccagctgct ttggcgtgcc
    77761 ggccgttgcg cccatggcca gggcaatgtt ctccgccacc gtcagtgtct cgaacagcga
    77821 gaagtgttga aacaccatgc ctacgccaag actgcgcgcc atggccgggt cacgcatctt
    77881 caacggctca ccctgccagc gaatctcgcc actgtcgggt tgggtgacgc cgtagatgat
    77941 cttcatcagc gtgcttttgc cagcaccatt ttcgccgagc aacgcatgga tttccccagg
    78001 cgcgatgctc aaatcgatac ggtcattggc caggcagccg gggtagcgct tgcttatacc
    78061 gctcagctgc aagcgtggaa cagaggggga actggacatg ggcagtggct caggaatgac
    78121 aactggcctg gctaaagcaa aaagctggcc agtgagtcag aaaagccgct gtgcagcgtc
    78181 agcgtaagtt tcgccataac gccgcgccag cgggtggctc cagcttggtg cgcagtcttg
    78241 atggctggtg cgttttggcg catcgcggcc aaggccgctc ctacaaaagc gtgccgcgcc
    78301 cggcaatcgc ggtgaggtcg ctatcccttg taggagcggg cttgcccgcg ataaatcctg
    78361 agcccgacgc aggctcataa ccacaccgca tcccacaacg ggtaatcgcc aatgcgttgc
    78421 accaggcctg ctcgcagcgg attggcgacg atataacgag ccgccgattg caggtcttca
    78481 tcctgacgca gtgccctgtc gtgataaccc ggctgccaga tcgggccctg tgtgccgagg
    78541 tactgattga tctgccgtgc gctgttgctt ttgatacggc gcatcagttt atccagagag
    78601 cctgtctgca tctgcacaag ccaatgcagg tgatcgggca tcaccaccca ggccagagac
    78661 gcaacctgtt gttgctcatg ggctgagcga agttctttta ccagcaaccg tgccaatgtg
    78721 tagtcattga acagcgggcg gcgttgcagc agggtggtgg tcagcaggta aatgcggtct
    78781 ggctctgaat agcggccgcg ccgcaggttg cggccatggg gttttacagg cattccattg
    78841 cctcttgtgg tgtatccgag agtgatcgcg ggcaagcccg ctcctacaaa agcgtgcagc
    78901 gcctcgccat cacggtcacg ccgctccccc tgtaggaacg gggggaacgc ccagttattg
    78961 cccgcgataa ttgctgaagt tggccgcaga ctcaccgcca caatgcagaa gcgtagtcga
    79021 tctattgcgg ccgcagttca ctccgcccac ggctgatatc cgccagcagt cgctgcagct
    79081 cggcgacccg tggggcgggg atggctaggt gcagctcggc ccctgtggcg tcgtagtctt
    79141 cgcttgtcag caggcagtcg agttccgtca ggcgtgcctt gagcagcggc agttcggcga
    79201 acaggcaatg gcagtggtac tgctcgcggg ccaccaactc gatgcgcggg gcgctttgca
    79261 ggcatttatt ggcgctgccg ccgtaggcgc gagccaggcc gccggtgccg agctggatgc
    79321 cgccgtagaa gcggatcacc agcaccgcga cctgatccat ctcctgggtt tcgatcgctg
    79381 cgagtatcgg ccggccagcg gtgccgccgg gttcgccgtc gtcgctgaag cggtactgct
    79441 ggccaacttt ccaggcccag cagttatggc tggcgctggc atcgctgtgc gccgcgataa
    79501 acgcctgcgc ctcggccgca ctggtgaccg gcgcggcgag ggcgataaag cggcttttgc
    79561 gaatctcctc gcggtactcg caagggccga gcagggtgaa gggcatcagg cgtcagctat
    79621 caaacgtctg gggcgggagg cgtcaggccg cagcccttga ggatgatgcg gatcagctgg
    79681 tcgcccgcca cttcatagtc ctgcttggtc aggcgcgagg tgccggtaac gcggcagatt
    79741 tgcgaagaga agtcggcgta gtgctgggtg ctgccccaca gcaggaagat caggtgcacc
    79801 gggtccactg ggtccatctt gccggcctcg ctccaggctt caaacaccgc ggcgcggcca
    79861 cggaaccagg tttgataatc ctggctgagc atctgtgaca gggtttcgcc gccgctaatc
    79921 acttccatcg catagatgcg cgaggcctta gggtgtcggc gtgagaattc catcttggcg
    79981 cggatataag cggccagcgc ggtggccgga tcatcgtcca cgctcaggtt gttgaaggtg
    80041 ttgtcccaca gctcaagaat gttgatcagc accgcctgat acagccccag cttgttgctg
    80101 aagtagtaat gcaggttggc tttcggcagg ccgacgttct gcgcgatggt gttcatgctg
    80161 gtgcctttga agccgtggcg ggcgaactct tcctcggcgg ccttgatgat cgcctcttcg
    80221 ttcttctgcc gaatccggcc ggcaggtttg ttgttcggat cggctcggtg cgcaagtact
    80281 tcaacagtca tgggcggttc cgcagtggtc aggttttcgg acgactgcac tgataaccca
    80341 gcgccgcttg ggtgacaagc gcaaggggca gaaaaactga cctaggtacg ctgtaaccag
    80401 gcttttgcac cagggttgtg cgtgtaaatg gcgtgctgcg gtggttttgg ggctggtgct
    80461 gtacctggta gcccagatgt aatccgggaa atttacggcg cgacatcgaa cgcttcccgg
    80521 attgcatccg ggctacgagt ggttttgctc gagcagtgcg ggggccgcct gctcccggcg
    80581 cccacccaga taccagccca gcgcgcccca ggcggctgcg cagagggcgc ctgctatggc
    80641 ggcgatcaat acgccattag cgagcatgtc gagcagggcc ttggcccagg cgctgacggc
    80701 gtcgccggcg cggtagacca cggtgtcgat aaagttcttg gccttgtatt tactctcggc
    80761 gtccagcggg gcgaagagca tttcccggcc gggacgcacg aaagcgtatt caccgacccg
    80821 gcgcacaatc atcaaggctg cgagcatggc gaaggttggc gccagggcca ggccgataaa
    80881 gcccaggcag gccagcagcg gcaccccggc gagcagggcg cgaatgccaa agcgctgggc
    80941 gatgcgcccg gtgataaaca gctgtgcgag caaggacagc gcctgcacgc tgaagtcgat
    81001 caggccgaat acgcggatct gttcgttgcg ctcggtgaag gtctccgcca ccaggcgcgc
    81061 ctgctcgaag tagagaaagg tgctggcggt ggctaacaga atgacgaagg cgctgatgcc
    81121 cagcagatag ggcgaggcga gcacgcgcgt caggccgcta aacggattgc cgatcactgg
    81181 tcggtgcggg ctttcgctga ccggtgcgcc gggccgaccg gcaccgccaa tcgcgcgcca
    81241 ggccatcagt tgctgcttgg ccagaatcgc catacccagc agcactgccg ccagcaggat
    81301 cagcccgctc tgcccgaagc gctcaatcag cagcgcgccg agcgctggcc cggccaagcc
    81361 gccgcagctg gccccagcgg cgataaaggc gaacagacgc ctggcttggg cgctgtcgaa
    81421 cacatcggcc atcaggctcc aggccaccga gacgacgaac aggttgtaga ccgagatcca
    81481 cacgtaaaac acccgcgcca cccagatgtc gtcgccgctg tgctgaaaca gcgcggcgaa
    81541 ggccagcaga ttgaggataa aaaatccgta cacccaatcg atatagcgcg cccgcgccac
    81601 cgtcgagtta agccaggcaa acagcggcac ggcggtgagc atcactagaa aggtcgcggt
    81661 aaacagccat tgcaggtttt ccacgccgcc gcgaatgccc atggcctcgc gaatcggccg
    81721 cagcatgaaa tagccgctga acaggcagaa gaacagacca aacccgcaca gcaccgccag
    81781 cagctcggcg ggccgagcat tgatcgcctg ggccaggcgt tgcgccgggc tcatcgctga
    81841 tccttctggt tgcgggcgcg cccgtgcata tcagctgaac gcctggacaa tcttctcgcg
    81901 cagcgccgca tccggcaggc ggccgatgcc ggccttgagg ttgtcggcca tatagcgcgg
    81961 gttggaggtg gccgggatca ccaccgtcac cgccgggttg gcgaggataa atttgagcaa
    82021 cagctgcgcc caggacgtgg cgtcggtctc catcgcccag ctgggcagcg atttgccttt
    82081 gacccggctg agcagctgcg cgcgctggaa tggccggttg atcagcgtgg cgatgccgtg
    82141 gtcggcgcag tagggcagca ggcgcttctc ggcattgcgt tcgcccaccg agtagttgag
    82201 ctggacgaaa tccactgact cgttttccag cacctccagc aggtcgtcgt gggccgactc
    82261 gatgtaatgg gtgatgccga tatagcgcac cttgccctcg gctttcagtt cgcgcagcaa
    82321 ggccagctgg gtgcgggtgt cctgcaggtt atgcacctgc accaggtcga gcgtgtcggt
    82381 ttgcagcgcc tgcaagctgg cctcgatttg cgccatgccg cgctcgcggc cggtggcgga
    82441 aatcttggtg gcgaggaagg ctttgccctg ggtgttggtg cgtttcagca gctcgcctgt
    82501 caccgcttca gaggcgccgt aactcggcgc ggtgtcgatc agcctggcgc cgccgttgat
    82561 aaaggtgcgc atcaccgaat ccagaggttt caggctgtca ttggtgaggc cgacatcgaa
    82621 ggtgcgcgag gtgcccaggc cgatcaccgg cagcatctcg ccgttgctcg ggatggcgcg
    82681 tttcagcagt tggctggggt cggcgtaagc cagggaaggc agccagggcg tggcggcgag
    82741 agcgagcagg ctggcgctgc cctggatgaa gtgacgacgg ctaggcataa gctcctccgt
    82801 ggtgacactg cacaggtaaa gggatcaaag ccgcgccagg cgtggcttcg accgagcata
    82861 gacctgtgag tcgcgactcc gcccaacgtt gcctgagcag ttgttacctg gtgtgtcgct
    82921 atgcctcggc cagggcttca agaaaacttt ccagcaccag atgcgggcgg cggcctttgc
    82981 gggtgaccga ggccaggctg atgtcgtaga agcggctggt cggtttcagc acgcgcaggc
    83041 ggccctgttg cacccaggcc tgggcgtagt gatcgggcag gtagccgata tagcgcccgg
    83101 tgagaatcag aaaggccatg ccttcacggt ccgaggcgct ggcagtgcaa ttgagcgcct
    83161 ggtagtggct ctgtacttcg ggtggcaggc ggaaggtcgg ggcgatggct tcctgagcat
    83221 tcagccgctc atcttccagc tgctgatcat cgacatagaa cagcggatgg ccgaccgcgc
    83281 agtagagcag cgaacgctcg tcgtacagcg tctggtattc cagcccggac agcgcgctgg
    83341 cctgcggcac tacgccgata tgcaggcgac cgtcgagcac gccttgctcg acttcgctcg
    83401 gcggggtcat gcggatatgg atatgcacgt ccgggccgcg ctccttcagc cgtgccaggg
    83461 cgttggtgat gcgcatatgc ggcaccgtca ccaggttgtc ggtcaggccg atgttcagct
    83521 cgccgcgcag gtgctggtgc aggccgttga cctcggtgcg aaagctttcc agcgctgcca
    83581 gcagctgctg ggtgctctgg aacacctcgc ggccttcctc ggtcagggca aagccggcgc
    83641 ggccgcgctg gcacaggcgc aaaccgaggc gctgttcgag gtcgctcatc tgctggctga
    83701 tcgccgaacg gccgatgccc agggcgcttt ccgcagcgga aaagccgccg cactccacca
    83761 cgctgcgaaa cagcttgagc aggcgaatat caaaatcact gacctgggcc agcgggtcgg
    83821 ggcggcgggt gctcatgttg ggaggctcat tgtttagtga aggcttaact aaagataaga
    83881 agagtcgtgt tttcctgact ctaagcccgt ggcaccttta ctggcaacag attcgctttc
    83941 tctgtgggag gggtcgggcg gcgaatccgc tttagccgcg aggatcaaca gctgtcgcgg
    84001 ctaaagcccc tcccacagga cctaaattgc tcagtcgccg atacgcgagg ccgcaccatg
    84061 aacatgccgc agaccgaaac cccgtccctg gccagccagc tcaagctgga tgcgcactgg
    84121 atgccgtttt ccgccaaccg caacttcaag cgcgacccgc gcctgatcgt cggtgccgac
    84181 ggcaactact ttatcgacga caaaggtcgg cgcattttcg acagcctgtc cgggctgtgg
    84241 acctgtggtg ctgggcacaa ccgcaaggaa attcaggaag ccgtggccaa gcagctgggc
    84301 accctggatt acgcgccggg cttccaatat ggccatccgt tgtcttacca gctggctgag
    84361 aaaatcaccg agctaacccc ggccggcctc gatcatgtgt tctacaccga ctccggctcc
    84421 gagtgtgccg acacctcggt gaagatggcc aaggcctact ggcgcctgaa aggccagccg
    84481 agcaaaacca aatttatcgg ccgtgcccgt ggctaccacg gtgtgaacat cgccggcacc
    84541 tcgctgggcg gcatcggcgg caaccgcaag atgttcggcc agatgatgga cgccgaccac
    84601 ctgccgcaca ccctgcaagc gcacctggct ttcaccaagg gcatggcggc taccggcggt
    84661 gtcgagctgg ccaacgagct gctgaaactg atcgaactgc acgacgcctc caacatcgcc
    84721 gcagtgatcg tcgagccgat gtccggctcc gctggcgtga tcgtgccgcc ggttggctac
    84781 ctgcagcgcc tgcgcgaaat ctgcactcag cacaacatcc tgctgatctt cgacgaagtg
    84841 atcaccgcct acggccgtat gggcaagtgg accggtgccg aattctttgg cgttaccccg
    84901 gacatcatga acaccgccaa gcaggtcacc aatggcgcga ttccgctggg cgcagtgatc
    84961 gcctccagcg agatctacaa cacctttatg aaccaggcga ttcccgagca catggtcgag
    85021 ttcggccacg gctacaccta ctcgggccac ccggttgcct gcgccgccgg cctagcaacc
    85081 ctggaactgc tcaaacgcga caacctgatc gagcagtccg ccgccctggc accgatcttt
    85141 gaagacaagc tgcatagcct gaaaggcagc aagaacgtca tcgacatccg caactgcggc
    85201 ctggctggcg cgatccagat cagcccgcgt gacggcgacc cggtgatccg tcccttcgag
    85261 gccggtatca aactgtggaa cgccggtttc tacgtgcgct tcggcggcga caccctgcag
    85321 ttcgggccaa ccttcaacac cacgcccgag cagctggact ccgtgttcaa cgccgtcggc
    85381 gatgtgctga acagccttga ttaagccaaa ccctattccc tgtaggagcg ggccatggcc
    85441 gcgaaacgca tcgcggccat gggccgctcc tacacaacaa gatccgttat taaggaattg
    85501 ccatgaccat cgttaaccac ctgatccacg gcgaactgac tgccggcacc ggccgtagcg
    85561 ccgatgtatt caacccgtcc accggcgcgg ctaccaagaa agttgccctg gccgaccgcg
    85621 ccaccatgca actggccatc gactcggcca agagcgcctt cccggcctgg cgcaataccc
    85681 cgccagccaa gcgcgcccaa gtaatgttcc gcttcaagca actgctggag cagcacgaag
    85741 cgaccatctc caagctgatc agcgaagagc acggcaaaac cctggaagac gccgccggtg
    85801 aactcaagcg cggtatcgaa aacgtcgaat tcgcctgcgc cgccccggaa attctcaagg
    85861 gcgagtacag ccgcaacgtc ggcccgaata tcgacgcctg gagcgacatg cagcccatcg
    85921 gcgtggtcgc cggcatcacc ccgttcaact tcccggccat ggtgccgctg tggatgtacc
    85981 cgctggccat cgtctgcggc aactgcttta tcctcaagcc gtccgagcgt gacccaagct
    86041 ccaccctgta tatcgcccaa ctgctgatcg aggccggtct gccaaaaggc gtgctcaacg
    86101 tggtgcacgg cgacaaggaa gcggtggacg cgctgatcga agcgccggaa gtcaaagccc
    86161 tgagctttgt tggctccacg ccgattgccg aatacatcta cagcgaaggc accaagcgcg
    86221 gtaagcgcgt acaggccctg ggcggcgcga agaaccacgc cgtactgatg cccgatgccg
    86281 acctggacaa cgccgtcagc gcactgatgg gcgcggccta cggttcttgc ggcgagcgtt
    86341 gcatggccat ctccgtggcc gtgtgcgtag gcgaccagat cgccgatgcc ctggtcgaga
    86401 agatcgtgcc gcaaatcaag gccctgaaaa tcggtgctgg cacctcctgc ggcctggaca
    86461 tgggcccgct ggttaccggc gcggcgcgtg acaaggtcaa aggctacatc gacgctggcg
    86521 tggcccaggg cgcaagcctg gtggtggacg gtcgcgacct ggtggttgca ggcaacgaaa
    86581 acggcttctt cgtcggcggc accctgttcg acaaggtgac cccggagatg accatctaca
    86641 ccgacgaaat cttcggccca gtgctgtgca tcgttcgcgt cggcagcctg gaacaagcca
    86701 tgcagctgat caacgaccac gaatacggca acggcacctg catcttcacc cgcgacggtg
    86761 aagcggcgcg cctgttctgc gacgagatcg aagtcggcat ggtcggcgtc aacgtaccgc
    86821 tgcctgtacc ggtcagctac cacagcttcg gcggctggaa gcgctcgctg ttcggcgacc
    86881 tgcacgccta cggcccggac ggtgtgcgct tctacacccg caaaaaagcc atcacccagc
    86941 gctggccgca acgcgccagc cacgaggcgg cgcaattcgc cttcccgagt aatggctaag
    87001 taacacccaa gggggctgat cgcaggatca gccccctttt tggatgtgga tgacgaccgt
    87061 aggagcggcc ttggccgcga tctttcgcga ccatccgttt gcatgcgatc agtcccctcg
    87121 cccctctggg gagagggtta gggtgagggg atatctgccc tcacccccag cccctctcca
    87181 ctaggcgtgc ccccaataaa tgggagaggg gagcagtaca gctttacgaa acaaaccagc
    87241 cgcgcgggcg cggcgttggt gcagtgaccg caggttacgg caccccttgg gtgagcgggt
    87301 tcagggtttc ctgacccgta agcccgctct cccaagggca gcccacttca attattaatg
    87361 aagaggactc cttgcaatga gcaaacccat gattggcctt cccccgtgcc gttggcagct
    87421 gaccaaccgt gatatcggct ggtttcacct ggtcggtgag aagtacatcc acgccgtcac
    87481 tggcttcggt gccttcccgc tgatgatccc ggctttcggc gacgacctgg atatggacac
    87541 cgtgctggat agcgtcagcg gcatcatgtt cggtggctcg ctctccaacg tgcaccccag
    87601 cttctacggc gacgaccatc ccggcctcgg cctggccgac aaaccccgtg atgaaaccgt
    87661 actgcgcctg atgcgcgcct gtattgatcg cggtattccc ttccttggta tctgccgggg
    87721 catgcaggaa atgaacgtgc tgtttggcgg caccctgcac cgcgaagtgc atgaagttga
    87781 aggtcgtctt gatcaccgtg aggttaagga cgtaccggac gacgtagcct acggcccgat
    87841 gcacaacgta gccctgaccg agggcggtgt gttgcattcg ctgttcggcg agcggcagat
    87901 caaggtcaac tcgttgcacg gccagggggt tgatcgtctc ggcgagggct tacaggttga
    87961 ggccgtggcc gaggatgggc agatcgaggc ggtcagtgtg attggtgcca aggcctttgc
    88021 cgttggggtg cagtggcatc ccgagtatcg ctattgggag aaggcggatt acaacgcgct
    88081 gctgggggcg tttcatcagg cggcgaggga atatcaggtg aggaaacttg agcgtcgcag
    88141 cgaggctgcg ttggtctgag cctagtggcg atttaaacaa aaggcccatc gattgatggg
    88201 ccttttgttt tgagagttga tgggtatcgc gttgctcaac cccactcttc aggttcgcgg
    88261 tctgtcagca atgagcaaag ataagcttaa agttcggaga tttcactgat gatgttgtct
    88321 atgacttcat caattttgtg atacttccta taaatggact gatggagaga taatagtagt
    88381 ctgaactcac ttgggctgtt ttcgtcgtag tatatgcgct caatctgaat tccttgatcg
    88441 ttcaagatgc aaatgtctac tacatgctct tcttcgtcgc tgaattcatg tcctgccata
    88501 tagctatcta cagttattgc gccgcttcct gttttgcaaa tgaattcatt atctcttgag
    88561 gttctttccc attttatctt cgaagtacta gttttatcag ttaatgtgcc aattaggttt
    88621 ctgatttttt cgtccacaat cttatccttt gttctttgtg atgctttgaa tttcgttaag
    88681 cagatcgatg gttgcctcca gcgtgttatt tacgcgggct acctcttctc cgctcatttt
    88741 tgctctagtg tctttttcaa agccgttaat tataatattt agactgataa tttgatttgc
    88801 tgcgttggtg ccgtgagtgt ttttgatttc actatttgcg gcgttaacaa tatcctgcaa
    88861 tatctgcttt aattcctgga ttcgcggaat tgcaatctct ctttttccgc tgcggcagta
    88921 tatttgcact tcttgtgcga ctttaactcc tctcgcccaa tctgctatgc acgtaaggag
    88981 gagaacttgc gactttgtct ccttggctgt ttcagaaata acctctgatt cttttctcat
    89041 taaagctatt tgtgcgattg cgtatgctaa gcccgtaatg gttccaagtg tgccgaatat
    89101 tgaaatgtac ttgttttgga attcagttag ggtgactcct gcaagtaaat atgagccgct
    89161 ggctatacct atgatcgaga ctattaacgc tattctcatt tgtctgcgtc caatttcatt
    89221 cctcgttgag cccgtaggat gggttgagcg tagcgatacc aatcaataat ggctatacct
    89281 attcaccgct caacccccgc ccaatcccca tggcaataag cccgcccgta cataacgatg
    89341 aaacgacgac cacagctaat tgaccaggcg ctgcatacct gttgcgcggt ctaacggttc
    89401 cgcccttacg gcggctcact tttggcaaac gccccaaaag taagcaaaag gtcttgcccc
    89461 ttgcatccgg gtctcgctgc gctcgacttc cctcgtttca tcgttgctcc agaggcccgc
    89521 cgcgaagggc catccctggc ccatcgcggc tctcgcggca tccatgccgc tcaacccctt
    89581 acgcaacgat tccactcggc cttctgatgg ggcgttttgg tgtcgtctgt gtaatcgcgg
    89641 attgaaaagc aaaaagcatg gcgtgtttca cccgccctac aggttacgac tacgaactcg
    89701 taggagcggc ccatggccgc gatgcttttg gtctttgttc tgctcttgcc tttcgttctg
    89761 ctatccagaa atcgcccagt cagcgcggtc cccgaatccc gtcaggaggc cgaaacgtag
    89821 cgcagcggag taacagccga aggctggccc gaagggtgag cgcagcgaat caagtaactc
    89881 gccgtaaggg cgaaaccagt aagtagggcc aatcaatcag cggcgggtgt accgtaatcg
    89941 tcatgccgta agccctaatc tgccgccact tgtggcagtg tcgatttgct cggctttcga
    90001 tttcagacgg cgaacgcttt gcgacctatc gcggtggaca agtttcgcgt tgtccacccc
    90061 atatatgatc accaccgcga cagtggtgcg cagtgcccgc gtgatgcctg ggcggccagc
    90121 ctgaacctgg caatgggcgc cacgggggga tttttacact agagtgaagg cattccctgc
    90181 gcaggccttg aacatgagcg attacccacg cccggttaac gatggcgcgc gcatacgtgc
    90241 gctggacgcg cttgagctga tgggctccgc gccggagcgg gagtttgatc gcgtggtgca
    90301 gatggccgcg cggattttcg atgtgccgat tgctctggtg tcgctggtgc accgcgacca
    90361 tcagttcttc aaggcgcgag tggggctaga tgtctgtgag acgagccggg agttgtcctt
    90421 ctgtacctac gcaatcatgg gccagacggc gtttgtcgtg gctgatgccc atgacgatga
    90481 gcgcttcagt gccaatgcgc tggtaacggg cgcaccgaat attcgctttt atgcggggtt
    90541 tccgctgtta acggccgacg gtcatgcgct gggcagcctg tgtgtgatcg accagaaacc
    90601 gcggcatgat ttttccgtgc gtgacgagca gatcctcagg gacttggcag cgctgctcag
    90661 cgagtacatc gaccagcgct gtttcgcgcg cgagggcgat gagttgcgca gccgcttccg
    90721 gcatattgcg gccacttcac ccgacggcat catatgctcc aacagccgca acctgatcac
    90781 ttcctggaac gaggcggccg agaagctgtt cggctactcg gcgcaggagg ccatcggcca
    90841 gacgctggat ctgatcatcc ctgccccgat gcgtgggcac catgccgccg gggttgcacg
    90901 ggttgccggc ggcggcgcgc cgcggctgat tggttcgtcg gtcaatgtgc atgcgctgcg
    90961 gcgcgacggt tcgcagtttc ccatcgagtt atcgttgtcg cagtggcagg aggagggtga
    91021 gcgctgtttt ggcgcgatca tccgtgacat taccgcacgc accgagctgg agaagcagct
    91081 caagcatgcg gcggaacacg attacctgac cgggctggcc aaccgtgcgc ggcttaaacg
    91141 ctgcatccag gaggcgctgg ccgcgcaaca accggccacc ctgctgctga tcgatctgga
    91201 tggcttcaag gatgtcaacg ataccctggg ccatagcgct ggtgacttcg tgttgcaggt
    91261 ggtggcggcg cgcttgcgcg aagcggtgcc gctggagcag atggtcagcc ggctcggtgg
    91321 cgatgagttt gtggtgtttg tgaccggcag taccgatcag gagtcggcgc gggctttggg
    91381 gctggagctg attggggtca tcgaggagca gatcgagttt gatgatcagc ccattttcat
    91441 cggggcgagc gtgggcgtgg cgatccactg cggcaccggt gaaagtgatg agcagttgct
    91501 gggcaatgct gacctggcgc tctaccaggc caagtccgat ggccgccatc tggtgaacat
    91561 cttcaaccct gagttgcgcc aaacggcttc tcgcaagggc gcgatcagtt cgtcgatgcg
    91621 ccaggcctgg gagtgtcagg aatttgagat gtattaccag ccgcaggtca acctggccga
    91681 cggttcgttg tgtggggccg aggcgctgat tcgctggaac catccaactc agggcgttac
    91741 cgcgccagcg gtgtttttgc cggtgctgga agctggtctg ttggccatcc ccgtgggtga
    91801 gtggattctg cgtacagcct gccggcaggc tgcgcagtgg cgcagcatgg gcattgggca
    91861 ctttcggatc ggtgtgaacc tgttcgcggc gcagtttcgc gcccgtgatt tcgcggacat
    91921 ggttaaagcc atcctggccg aattcaatct gccggccagc gctctggagc tggaaattac
    91981 cgagaacacc attttgcgca acgagcaacg cattatgcag ccgctgcgcc acctgcgcgc
    92041 gctgggggta gggatcgcct tcgatgattt cggcaccggg ttcgcctccc tcagcatgct
    92101 taaggattat cccgtcagcc gcctgaagat cgaccgatcc ttcgtcagcg gagccgaccg
    92161 cagcctgcgc gatgaggcca ttgtcgaggc ggtcagccgg ctggcagcgg ggtttcagct
    92221 tgaggtgatt gccgagggga tcgagacgca tgagcaggcc aggctgatgc agcactatgc
    92281 ctgcggcgaa ggccagggct acctgtatgg ccggccgatg accgccgacg acttcacccg
    92341 tcgttacctg ggtggtcggc ctgtcgccag cgagtagtcg gagcgctcct gcgctatagg
    92401 cgtgtcggtc atcggtgtgt ccggacagac caagtcgctg caactcatac ctattacagg
    92461 ccccgcaact gtgggtaatg ctgttcactt taggtcgcat gctttttact cccctctccc
    92521 atttatggga gaggggctgg gggtgagggc tgaaaacacc gccaaactgg aggcct
//
LOCUS       DASRUM010000295        13204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2478164, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000295 DASRUM010000000
VERSION     DASRUM010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 13204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13204
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2478164"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..293
                     /gene="arfA"
                     /locus_tag="VFF22_19960"
     CDS             135..293
                     /gene="arfA"
                     /locus_tag="VFF22_19960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072344 - rescue of stalled ribosome
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue factor ArfA"
                     /protein_id="HZX19131.1"
                     /translation="MAKKAKRGPNKAKSLIAQPLFRSRQETPSKGKGSYRREASQQNW
                     EVSYFLAA"
     gene            376..1659
                     /locus_tag="VFF22_19965"
     CDS             376..1659
                     /locus_tag="VFF22_19965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HZX19132.1"
                     /translation="MPYLLLRGLSHSATACLLLLLTACAEAPAQPLAAAPVTPSTAPA
                     SPVASEASSPTPLLSFDEWRILLRSDAIAAGIDAELFDRTFAGITLDPRVVTSDNSQP
                     EFTRPVWDYIDGAVSVSRIAQGRLLKAQHRPTLKAIRSTYAVDHEVLIAIWGMESNYG
                     SNIGSHNVIRSLATLAYDGRRQVFWRSQLLAALQILQAGDSPTQGLIGSWAGAMGQTQ
                     FMPTTFNQHAVDFDGDGKRDLWNSTPDALASAAHYLQASGWENGQPWGFEVNLPQGFD
                     YALADPEVRRTLAQWRILGVNPVRAIDTNEQSQATLFLPAGHRGPAFLLLNNFRSILK
                     YNNSTAYALAIGLLSDALDGNPGVQAAWPRNDRQLGRSERIELQELLASKGYEPGPAD
                     GIIGANTRKAVRAFQQSQGQPADGYPSHELLQQLR"
     gene            1816..2868
                     /gene="lipA"
                     /locus_tag="VFF22_19970"
     CDS             1816..2868
                     /gene="lipA"
                     /locus_tag="VFF22_19970"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HZX19133.1"
                     /translation="MSDTSPPKTVASGEKFRTTQGITAIKDGQKRRASSEPQVFEPKP
                     KWLRVKAPGGSRFEAVKRNVSEHRLSTVCQESHCPNMGECWSNGTATIMLMGSVCTRA
                     CRFCAVDTGNPNGWLDLEEPQNTAKSVELMALRYIVLTSVDRDDLDDGGASHYAACVR
                     AIKDNTPHVVVEALTPDFDGDLLAIERVVDSGLEVFAQNVETVKRLTHVVRDPRAGYE
                     KTLNVLAHAKRHRPDVLTKTSLMLGLGETDEEIIQTMDDLRAINVDILTLGQYLQPTR
                     NHLKVQRWVSPEEFNRFREIGLQKGFMEVAAGPLVRSSYRADKVFEKNNLGLAAPVPV
                     PGQQLDNSLIPALNLN"
     gene            complement(2951..3604)
                     /gene="lipB"
                     /locus_tag="VFF22_19975"
     CDS             complement(2951..3604)
                     /gene="lipB"
                     /locus_tag="VFF22_19975"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007946664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HZX19134.1"
                     /translation="MSLPLCFRELGQVDYQPTWHAMQRFTDSRGADTADEIWLLEHSP
                     VFTQGQAGKSEHVLFPGDIPVVQVDRGGQVTYHGPGQLVAYLLLDVRRSGIGVRELVS
                     RIERSLIDLLASYGVNANAKPDAPGVYVDGAKIASLGLRIRNGRSFHGLALNVDMDLQ
                     PFQRINPCGYAGMAMTQLADQVAGPIALSEVSARLREQLVKHLDYAQQQSLAGAIDC"
     gene            complement(3604..3873)
                     /locus_tag="VFF22_19980"
     CDS             complement(3604..3873)
                     /locus_tag="VFF22_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007975882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HZX19135.1"
                     /translation="MTDVKSHKIEFPCADYPIKVVGDSGANYKSTVIEILSQHAKVDL
                     STLAERQSSNGKYTTVQLHIEATSEDQLRDINSALRATGIVHMVL"
     gene            complement(3948..5123)
                     /locus_tag="VFF22_19985"
     CDS             complement(3948..5123)
                     /locus_tag="VFF22_19985"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HZX19136.1"
                     /translation="MNITSFTQRVFLSLCLLSASTAWANQPAIPSAPQLAAKSYVLLD
                     AASGKVLVENNGDQRLPPASLTKLMTAYIATLEIRKGKIGEQDLVPISEHAWRTGGAA
                     SGGSTMFLPLNSQATVDDLLHGVIIQSGNDASIALAEYIAGSEDAFADMMNETAARLG
                     MSNSHFMNSTGLPHPEHYSSAHDMAILARAIINEDQEHYAIYAQKEFLWNNIKQGNRN
                     LLLWRDKTVDGLKTGHTEEAGYCLVASAVRDGARMITSVFGTVSESARAAETQKLLTY
                     GFRFFETRTFYKQGQELAQAQVWKGTVRQVKAGLGADLSLTLPKGQLEKLQAGMILNP
                     QLTAPIAQGEVIGKVEVKLGDEVVHSADLIALEAVEEGSFFRRLWDSISLFFFGLFN"
     gene            complement(5245..6252)
                     /locus_tag="VFF22_19990"
     CDS             complement(5245..6252)
                     /locus_tag="VFF22_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HZX19137.1"
                     /translation="MSRFPFKLAAYGSLALLLASCSSTRAPQPVAPVQQPGGAISGPG
                     DFNRPHKDGAPWWDVDVSQIPDAVPMPHYGRVKASPYSVFGKQYYPIPDARRYQAVGP
                     ASWYGTKFHGQATANGETYDLYGMTAAHKTLPLPSYVRVTNLENGKSAILRVNDRGPF
                     YSDRIIDLSFAAAKKLGYAESGTARVKVEGIDPHEWWAQQGRPVPMVLAAPQQVAQAQ
                     PVAQPIAQPVEQYSPPPQQHAAAVLPVQIDAKKNASVGASGLYLQVAAFANPDAAELL
                     KAKLSETVAAPVFVSSVVRNQQILHRVRLGPVSSQSEAEQLQSSVRLANLGEPTLVKA
                     D"
     gene            complement(6252..7262)
                     /gene="mltB"
                     /locus_tag="VFF22_19995"
     CDS             complement(6252..7262)
                     /gene="mltB"
                     /locus_tag="VFF22_19995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HZX19138.1"
                     /translation="MQNARGWMVRTAPWLALVGVCAVAQPVLAGDYQQSPQVAEFIGE
                     MTRDYGFAGEQLGELFAEVERKQAILDAISRPAEKAKPWKDYRPIFITDKRISKGVEF
                     WKQHEETLARAEAEYGVPAQVIVAIIGVETFYGGNTGSWRVMDALSTLAFDYPPRAPF
                     FRKELREFLLLAREEQVEPLSMTGSYAGAMGLPQFMPSSFRAYAVDFDGDGHINIWSN
                     PVDAIGSVASYFKRHHWQPGQPVVSAATVTGERAEQGLTVGLDPVKNVAELRALGWTS
                     ADPLADDLPVTAFRLDGADGAEYWLGQPNFFVITRYNRSVMYAMAVNQLAELLVDARG
                     KN"
     gene            complement(7286..8383)
                     /gene="rodA"
                     /locus_tag="VFF22_20000"
                     /pseudo
     CDS             complement(7286..8383)
                     /gene="rodA"
                     /locus_tag="VFF22_20000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252691.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
     gene            complement(8422..10311)
                     /gene="mrdA"
                     /locus_tag="VFF22_20005"
     CDS             complement(8422..10311)
                     /gene="mrdA"
                     /locus_tag="VFF22_20005"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZX19139.1"
                     /translation="MPQPIRLKDHEKDGRLIRKRAVVGAVVVLLLTSVLVARMYYLQV
                     VQFEHHTTLAENNRIHVQPIPPNRGLIYDRNGVIIADNRPSFSLTLTRERAGDWPQVL
                     DVIVEVLELGADERALFERRVRQGRRPFEPVPVLFELSEEQIARVAINQFRLPGVEVA
                     AQLVRHYPLGEHFAHSVGYVGRINEKELKELDPVAYSGTHHIGKTGIERFYEDELHGE
                     VGYEEVETNARGRVLRVLKRTDPLPGKDIVLSLDIRLQEAAEKALAGRRGAIVAIQPQ
                     TGEVLAMVSQPSFDPNPFVTGIGFKAYADLRDSIDRPLYNRILRGLYPPGSTIKPMVA
                     VAGLDAGVVTPQTRVFDPGFYQLPGHSHKYRNWNRTGDGSVDMDYAIMRSNDTYFYSL
                     AHKMGIDRMHDYMTRFGLGQRVSLDMFEETAGLMPSRDWKRARYRQAWYPGETLILGI
                     GQGYMQTTPLQLAQATVLMATRGKWIRPHLAKTIQGRAPVDPNPLPDIVLRDPSFWDN
                     GRHGMEQVMHGPRGTARKVGDASVYRIAGKSGTAQVVAIKQGEKYDRSKVAERHRDHA
                     LFVAFAPADNPQIAVSVMVENGESGSGVAAPVVKQVMDAWLLDENGQLKAEYAPPVAV
                     EVAAP"
     gene            complement(10336..10803)
                     /gene="rlmH"
                     /locus_tag="VFF22_20010"
     CDS             complement(10336..10803)
                     /gene="rlmH"
                     /locus_tag="VFF22_20010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HZX19140.1"
                     /translation="MRIKLIAVGSRMPRWVEEGWQEYAKRMPSELSLELVEIPLTTRG
                     KNADVARMIRQEGEAMLAKVQPGERIVTLEVEGRPWSTEQLAVELDKWRLDARTVNLM
                     VGGPEGLAPEVQARSEQRWSLSPLTLPHPLVRILIGEQMYRAWTVLSGHPYHK"
     gene            complement(10816..11187)
                     /gene="rsfS"
                     /locus_tag="VFF22_20015"
     CDS             complement(10816..11187)
                     /gene="rsfS"
                     /locus_tag="VFF22_20015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HZX19141.1"
                     /translation="MQSEELVKLAITALEEIKAQDITTIDVRGKTSITDFMLIASGTS
                     SRHVKSLVDNVLEKVKEQGVRPIGTEGMDTGEWALLDLGDIVVHVMLPTARQFYDLER
                     LWQGAEQSRAQFSAEPGPEQE"
     gene            complement(11225..11869)
                     /gene="nadD"
                     /locus_tag="VFF22_20020"
     CDS             complement(11225..11869)
                     /gene="nadD"
                     /locus_tag="VFF22_20020"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HZX19142.1"
                     /translation="MARRIGLLGGTFDPVHIGHLRSAVEVAEALQLDQLRLIPSARPP
                     HRSTPQVSAVDRLAMVRLAVADAGVLQVDDRELQRDKPSYSIDTLESLRSELAADDQL
                     FLLLGWDAFCGLPSWQRWDELLQHCHILVLQRPDADSEAPEALRNLLAARSVSDPSAL
                     SGPSGQIAFIWQTPLAVSATQIRSLLASGKSARFLVPDAVLAYIHAHGLYRGAN"
     gene            complement(11883..13148)
                     /locus_tag="VFF22_20025"
     CDS             complement(11883..13148)
                     /locus_tag="VFF22_20025"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HZX19143.1"
                     /translation="MTESVLDYMTRLGQAARSASRVLARASTAQKNRALQAAAAALDA
                     ARSDLTAANELDLAAARAGGLEPAMVDRLALTPAVIDSMIEGLRQVATLPDPIGEIQG
                     MRYLPSGIQVGKMRVPLGVIGIIYESRPNVTIDAASLCLKSGNATILRGGSEAIHSNQ
                     AIARCIQLGLAEAGLPASAVQVVETTDRAAVGALITMPEFVDVIVPRGGKGLIERISR
                     DAKVPVIKHLDGVCHVYIDVAADLDKAIRVADNAKTQRYSPCNAMETLLVHAAVADKV
                     LPPLAAIYRDKGVELRGDAATRTLLGSDVLEASEDDWFAEYNAPILAIRIVDSLDAAI
                     EHINHYGSQHTDAIITENFSDARRFITEVDSASVMVNASTRFADGFEYGLGAEIGIST
                     DKLHARGPVGLDGLTSEKYVVFGDGHIRT"
BASE COUNT         2664 a         4258 c         3836 g         2446 t
ORIGIN      
        1 tgagcctgct gaccctgagc atctgcggcc aacgcctggg cctaacccgc tagccccaga
       61 ctctggactt tttgccacgt gcggatcatg cttcgcccgc ctgcgacgtg caggtcattt
      121 cccgaggaaa ggtcatggcg aagaaagcca aacgcggccc gaacaaggcc aaatccctga
      181 tagcccagcc actgtttcgc tcacgccagg aaacacccag caaaggcaaa ggcagctacc
      241 gccgcgaagc ctcccagcaa aactgggagg tttcatactt tctggctgcc tgaaaagccc
      301 gccacagccc gccagcaccc gcacaccagc tcaatcatgc taaggttacg gcctgcaaaa
      361 tgtgccgaga tcaccatgcc ttacctgctt cttcggggcc tgtcccatag cgccaccgcc
      421 tgcctcctgc tgcttctgac tgcctgcgcc gaagcgcctg cccagccact tgctgccgcg
      481 cccgtcacac ccagcaccgc ccccgccagc ccagtagcca gcgaggccag cagcccaacc
      541 ccgttactca gcttcgatga atggcgcatc ctgctgcgca gcgatgccat cgccgcgggt
      601 atcgatgccg agctgttcga tcgcaccttt gccggcatca ccctggaccc cagagtcgtt
      661 acctccgaca acagccagcc agaattcacc cggccggtgt gggactacat cgacggcgca
      721 gtgtcagtct cgcgcattgc ccagggccgc ctactcaagg cgcagcatcg gccaaccctg
      781 aaagccattc gcagcaccta cgccgtggac catgaagtgc tcatcgcgat ctggggcatg
      841 gaaagcaatt acggcagcaa tatcggcagc cataacgtta tccgctccct tgccaccctg
      901 gcctacgacg gccgccgcca ggtgttctgg cgcagccagt tgctggccgc actgcagatc
      961 ctgcaagccg gcgacagccc aacgcaagga ctgatcggtt catgggcggg cgccatgggg
     1021 caaacccagt ttatgccgac caccttcaat caacacgccg tggactttga tggcgatggc
     1081 aaacgtgatc tgtggaactc cacccccgac gccctcgcct ctgccgcgca ttacctgcaa
     1141 gcctcgggct gggaaaacgg ccaaccctgg ggctttgaag tgaacctgcc gcagggcttc
     1201 gattacgccc tggccgaccc ggaagtgcgt cgcaccctgg cgcagtggcg catcctcggg
     1261 gtcaacccag tcagagctat cgataccaac gagcaaagtc aggccacgct gtttctgcct
     1321 gctggccatc gcggtccagc cttcctgctg ctgaataact tccgcagcat cctcaagtac
     1381 aacaactcca ccgcctacgc cctggccatt ggcctgctca gcgacgcgct ggatggcaac
     1441 cccggcgtgc aggccgcctg gccacgcaac gatcgccaat tgggtcgtag cgagcgcatc
     1501 gaactgcaag aactactggc cagcaaaggc tacgaacctg gcccggcaga cggcatcatt
     1561 ggcgccaaca cgcgcaaggc cgtacgcgcc tttcagcaat cccaggggca acctgccgat
     1621 ggctacccca gccatgagct gctgcaacaa ctcaggtaag cgcccgcgca gcgtctagcc
     1681 gtgcgctctg ccctcgccta tgggcggcag aaccgcacgg cgcccgccgg actgaacggt
     1741 cagcttctgt gataagctgc gcggcggcca caagccgcct cgtccacgga gcctctagcg
     1801 gagcccagat tgccgatgtc cgatacctcc ccgcctaaaa ccgtcgccag tggcgaaaaa
     1861 ttccgtacca cccaaggcat caccgcgatc aaggacgggc aaaagcgccg cgcctccagt
     1921 gagcctcaag tgttcgagcc caaacccaag tggctacgcg tcaaggcgcc tggcggcagc
     1981 cgcttcgagg cggttaagcg caatgtcagc gaacaccgcc tgagcaccgt gtgccaggaa
     2041 tcccactgcc cgaacatggg cgaatgctgg tccaacggca ccgccaccat catgctgatg
     2101 ggctcggtgt gcacccgcgc ctgccgcttc tgcgcagtag acaccggcaa tcccaacggc
     2161 tggctggacc tggaagaacc gcaaaacacc gccaaatcgg tggagctgat ggcgctgcgc
     2221 tatatcgtac tgacctcggt ggatcgagat gacctggatg acggcggcgc cagccactat
     2281 gcagcctgcg tacgggcgat caaggacaac accccgcacg tggtggtaga agccctgacc
     2341 ccggacttcg acggcgacct gctggccatc gaacgcgtgg tggattccgg ccttgaggtc
     2401 ttcgcgcaga acgttgagac ggtcaaacgt ctcacccacg tggtacgcga cccacgtgct
     2461 ggctacgaaa agaccctaaa cgtattggcc cacgccaaac gccaccgccc ggacgtactg
     2521 accaagacca gcctgatgct gggtctgggt gagactgacg aagagatcat ccagaccatg
     2581 gatgacctgc gcgccatcaa cgtcgacatc cttaccctcg gtcagtacct gcaaccgaca
     2641 cgcaaccacc tgaaggtgca gcgctgggtc agcccggaag agttcaatcg tttccgcgag
     2701 atcggcctgc agaaaggctt tatggaggtc gccgccggcc cgctggtacg ttccagctac
     2761 cgcgccgaca aggtgttcga gaagaacaac ctgggcctgg ccgccccggt gccagtgccg
     2821 ggccaacagc tcgataacag cctgattcct gccctcaatc tgaactgatt gagccacaca
     2881 acgcctgacc ttgggctttg cctggggtca ggcgtttgtg tatctgctct gcctatctgc
     2941 cctgcccgct ctagcagtct attgcgcctg ccaggctctg ctgctgcgcg tagtcgaggt
     3001 gcttgaccaa ctgctcacgc agccgcgcac tgacctcgga aagcgctatc ggccccgcca
     3061 cctgatcggc taactgggtc atggccatgc cggcatagcc acaagggttg atacgctgga
     3121 agggctgcag atccatatcc acattcagcg ccaggccatg gaaggatcgg ccattgcgga
     3181 tacgcaggcc gagcgaggcg atcttcgcgc catccacata aaccccaggt gcatcgggct
     3241 tggcgttagc attaacgcca tagctggcga gtaggtcaat caggctgcgc tcgatgcggc
     3301 taaccagctc gcgcacgcca atgccgctac gacgcacatc cagcagcagg taggccacca
     3361 gctggccggg gccatggtag gtcacctgac cgccgcgatc gacctgcacc accgggatat
     3421 cgcccggaaa cagcacatgc tcggacttgc cggcctggcc ctgggtaaat accggggagt
     3481 gttcgagcag ccagatttcg tcagccgtat cggcgccacg gctgtcggta aagcgctgca
     3541 tcgcatgcca ggtaggctga tagtcgacct ggcccagctc acgaaagcag agcggcaggc
     3601 tcatcagagc accatgtgca cgatgccggt cgcgcgcagg gcgctattga tatcgcgcaa
     3661 ctgatcttcg ctggtggctt cgatatgcag ctgtacggtg gtgtacttgc cgttgctgct
     3721 ctggcgctct gccagggtgc tgagatcgac cttagcgtgc tgactaagga tttcgatcac
     3781 cgtacttttg tagttggcgc cactgtcgcc cacaaccttg atcgggtagt cggcgcaagg
     3841 gaattcgatt ttgtgcgatt tgacgtcggt catggcagta acggcctcgt aagccgtggc
     3901 aacaacaacg cccctgcggg tccggcgggg gcgcgctggt cacgctatca gttgaacaat
     3961 ccgaagaaga acaggctgat gctatcccac aggcggcgga agaaactacc ttcctcaaca
     4021 gcctcaaggg cgatcagatc ggcgctgtgc accacttcgt cacccagttt cacttcaacc
     4081 ttgccgatca cctcaccctg ggcaatcggg gcggtcagct gcggattgag gatcatgccg
     4141 gcttgcagtt tttccagctg gcccttcggc agggtcaagc tcagatcagc tccaaggccg
     4201 gctttaacct gacgcacggt gcctttccac acctgggcct gggccagttc ctggccttgc
     4261 ttgtagaagg tgcgggtttc gaagaagcgg aagccatagg tcaacagctt ctgggtttca
     4321 gccgcacggg ccgactcgct gacggtaccg aataccgagg tgatcatccg cgcgccatca
     4381 cgtacagccg aagccaccag gcagtagccc gcttcttcag tgtgaccagt tttcaggcca
     4441 tcgaccgtct tgtcgcgcca cagcagcagg ttacggttgc cctgcttgat gttgttccag
     4501 aggaactcct tctgcgcgta gatggcatag tgctcctgat cttcattgat gatggcgcgg
     4561 gccaggatgg ccatatcgtg agcactggag taatgctccg ggtgtggcag gccggtggag
     4621 ttcataaagt ggctgttgct catacccaga cgcgcagcag tctcgttcat catgtcggca
     4681 aaggcgtctt cgctgccggc tatgtactcg gccagtgcga tactggcgtc gttgccggac
     4741 tggatgatca cgccatgcag caggtcatcc acggttgcct ggctgtttag cggcaggaac
     4801 atggtcgaac caccggatgc agcaccaccg gtacgccagg cgtgctcgct gatcggtacc
     4861 aggtcctgct cgccaatctt gcccttgcgg atttccaggg tggcgatata ggcggtcatc
     4921 agcttggtca ggctggccgg cggcagacgc tgatcaccat tgttttccac cagcaccttg
     4981 ccgctggcgg catccagcag cacataggat ttggccgcca actgtggcgc cgaaggaatg
     5041 gctggctgat tggcccaggc ggtcgatgcg ctgagtaggc aaagtgatag gaaaacgcgt
     5101 tgcgtaaagc tggtgatatt catccgtctc tctagattgc tgatggtgaa caggcccagt
     5161 gggcataagg tgctgatccc ccagccaacc gttggctggc cggagctgtt tgtttagtcc
     5221 cgcataagaa ccataagtgc cctgtcagtc ggccttgacc agggtcggtt caccgagatt
     5281 ggccaggcgc acgctgctct gcaattgctc agcctcgctc tggctgctca ccggccccag
     5341 acgcacgcgg tgcaatatct gctggttgcg caccactgag ctgacgaaca ctggtgcagc
     5401 caccgtctcg ctcagcttgg ccttgaggag ctccgcagcg tccggattgg cgaaggcggc
     5461 cacttggaga tacaggccag aggctccgac tgaagcgttt ttttttgcgt caatctgcac
     5521 ggggagcacc gcggcggcat gctgctgcgg tggcggtgaa tattgctcaa ccggctgcgc
     5581 gataggctgt gccaccggct gggcctgggc cacttgctgc ggcgcagcca ataccatcgg
     5641 caccggacgc ccctgctgag cccaccactc gtgcgggtcg atgccctcaa ccttgacccg
     5701 cgcggtgccg ctttcggcat agccgagctt cttcgctgcg gcgaaagaca agtcgatgat
     5761 ccgatcggaa tagaacggcc cacgatcgtt gacccgcaga atcgcgctct tgccgttttc
     5821 cagattggtt acccgcacgt agctgggcag cggtagggtt ttgtgtgcgg cagtcatacc
     5881 gtacagatcg taggtttcac cgttagcggt ggcctgaccg tgaaatttgg tgccatacca
     5941 ggacgccggg ccgaccgcct gataacgacg ggcatcagga atcgggtagt actgcttgcc
     6001 gaacacgctg taggggctgg ccttgactcg gccgtaatgt ggcattggca cagcatcggg
     6061 aatctgcgac acatcgacat cccaccaggg cgcaccatcc ttgtgtggac ggttgaaatc
     6121 acccggcccg gaaatcgccc cacccggctg ctgcaccggt gcaaccggct gcggcgcacg
     6181 ggtagacgag cagctggcca gcagcagcgc caagctgcca taagcagcca acttgaaagg
     6241 aaaacgcgac attaattctt accccgtgca tcaaccagca actcggccag ctgattgacc
     6301 gccatggcat acatcacact gcgattgtag cgggtgatca caaagaagtt aggctggccc
     6361 agccagtact cggcgccgtc ggcaccgtcc agacggaagg ccgtcaccgg caaatcgtca
     6421 gccagcgggt cggcactggt ccagccaagg gcacgcagct cagcgacatt cttcactggg
     6481 tccaggccca cagtcaggcc ttgctcagcc cgttcacccg tgacggtggc ggcgctgacc
     6541 acaggctgcc caggctgcca gtgatgacgt ttgaagtaac tggccacgct gccgatggca
     6601 tcgaccggat tgctccagat attgatatgg ccgtcgccat caaagtccac ggcataggca
     6661 cggaagctgc tcggcataaa ttgtggcagg cccatggcgc cggcgtagga gccagtcatg
     6721 ctcagcggct caacctgctc ttcgcgcgcc agcaggagga actcacgcag ctccttgcgg
     6781 aagaacggcg cgcgcggagg gtaatcgaag gccagggtcg acagggcgtc catcacccgc
     6841 cagctgccgg tattaccgcc gtagaaggtt tccaccccaa tgattgccac gatcacctgg
     6901 gctggtacgc cgtattcggc ctcggcacgg gccagggttt cctcgtgctg cttccagaac
     6961 tcaacgccct tgctgatgcg cttgtcggtg ataaagattg gccggtagtc cttccacggc
     7021 ttggccttct ccgccggacg cgagatggcg tcgaggatcg cctgcttgcg ctcgacttcg
     7081 gcgaacagct cgcccaactg ctcacctgca aagccgtaat cacgggtcat ttcgccgatg
     7141 aattcagcca cctgcggcga ctgctgataa tccccggcca gtacgggctg agccactgcg
     7201 cacaccccga ccaacgccag ccaaggtgca gtacgtacca tccaaccacg cgcgttttgc
     7261 attgaattca tagtcccaat caaacttatg ccatccactt acgatgagtg tggatcgaca
     7321 tcaaaattcc aaatcctgcc agcagggtaa tcagcgaggt gccgccatag ctgataaacg
     7381 gcagcggtac gcccaccacc ggcagcaggc cgctgaccat gccgatattg acgaagacat
     7441 acacgaagaa ggtcatggtc agcgcgccgg cgagaagctt accgaacagc gtctgcgctt
     7501 gcacggtaat caccagcccg cgcgcgatca acagcatgta caccagcagt aacaaacaca
     7561 caccgaccag gccgaattct tcggccagca ccgcgatgat aaagtcagta tggctctccg
     7621 gcagaaaatc caggtgcgac tgggtgcctt gcagccagcc tttgccgagc accccacccg
     7681 aaccaatcgc ggccttggac tggatgatgt tccagccact gcccagcgga tcgctttccg
     7741 ggttgaggaa tgtcagcaca cgctgtttct gataatcacg cataacaaac agccacatgc
     7801 cgatggcaat cggcaccacc gccgccaccg caccaattac ccagcgccat tgcagcccgg
     7861 ctataaacag tacgaagcca ccggaagcaa tgatcagtag tgaggttcca agatccggct
     7921 gcagcagaat cagcacaaag ggcacgatga tcatcgccag gctgatgcag atatgtttga
     7981 ggcgtggcgg cagactgcgg ttggacaggt accaggcgat ggtcgccggc atgatgatct
     8041 tcataaattc cgagggctgg aagcgaatca ccccggggat attgatccag cgcgtggcgc
     8101 ccatggcgtt atggcccatg acgtccaccg ccaccagcaa ggcgacaccg ccgacatagg
     8161 ccaacggcac ctagcgcgcc ataaagcgcg gctcaaactg ggcgatgatc aacatgccga
     8221 ccaagccgat accaaaggag cttgcctgtt tcatcagcaa gtcgacgttc ttgccactgg
     8281 ccgaatacag gataaacagg ctaccggtgg ccagaatcaa cagcagcaac agcagaatgc
     8341 cgtctatatg caggcgctgc aggatggttg cgcggcggcg cataaggtct tcggaggaca
     8401 ggttgcggtc gaaattactg ttcatggcgc agcgacctcg accgccactg gcggagcata
     8461 ttcggctttc aactgaccgt tctcatccag cagccaggca tccatcacct gcttgaccac
     8521 tggcgcggca accccggaac cggattcacc gttctcgacc attaccgaca cggcaatctg
     8581 cgggttatcc gccggcgcaa acgcgacgaa cagggcgtgg tcacgatggc gctcggcaac
     8641 tttgctgcgg tcgtatttct caccctgctt gatcgccacc acctgagcgg tgccgctctt
     8701 accggcaatc cggtagaccg aggcgtcacc taccttgcgc gctgtaccgc gcgggccgtg
     8761 catcacctgc tccataccat gacgaccgtt atcccagaac gagggatcac gcagcacgat
     8821 atccggcaaa ggattgggat ctactggcgc acggccctga atggtcttgg ccaagtgcgg
     8881 acgaatccac ttgccgcgcg tggccatcag caccgtggcc tgggccaact gcagcggcgt
     8941 ggtctgcata tagccctgac cgataccgag aatcagcgtc tcgcctgggt accaggcctg
     9001 ccggtaacgc gcacgcttcc agtcccgcga aggcatcagc ccggcggttt cttcgaacat
     9061 gtccagcgac acacgctgac ccagcccgaa acgggtcata tagtcatgca tgcggtcgat
     9121 gcccatcttg tgcgccagcg aataaaagta ggtgtcgttg gagcgcatga tcgcgtaatc
     9181 catatccacc gagccgtcgc ccgtgcggtt ccagttgcga tatttgtggc tgtgaccggg
     9241 caactgatag aagccggggt cgaacacccg ggtctgcggc gtgactacgc cagcatccag
     9301 accggccacc gcgaccatcg gcttaatggt cgagccgggc ggatagagac cgcgcaaaat
     9361 gcggttgtac agcggccggt cgatggagtc gcgcaggtcg gcataggcct tgaagccgat
     9421 accggtaaca aaggggttgg ggtcgaagct cggctgactg accatcgcca gcacttcacc
     9481 ggtctgtggc tggatcgcca caatcgcgcc ccggcgaccg gccagggcct tttcggcggc
     9541 ctcctgcaag cgaatatcga ggctcagtac gatatccttg ccgggcagcg gatcggtgcg
     9601 tttgagcacc cgcaatacgc ggccacgggc gttggtttcg acttcctcgt aacccacttc
     9661 accgtgcagc tcgtcttcat agaagcgctc gataccggtt ttgccgatat ggtgcgtgcc
     9721 gctgtaggcc accgggtcga gctctttcag ctctttttcg ttgatccgcc cgacataacc
     9781 caccgaatgg gcaaaatgct cgcccagcgg gtaatgccgc accagctgcg ctgcgacttc
     9841 cacgcctggt agacggaact gattgattgc cacacgggca atctgctcct cggacaattc
     9901 gaacagcacc ggcaccggtt caaacggccg ccgcccctga cgcacacgcc gctcgaacag
     9961 cgcgcgctca tccgcgccca gttccagcac ttcgacaatc acgtcgagca cctgcggcca
    10021 gtcacccgcg cgttcgcgag tcagggtcag gctaaagctg ggccggttat cggcgatgat
    10081 caccccgttg cggtcgtaaa tcagcccgcg gttcggcggg atcggctgca cgtggatgcg
    10141 gttgttttcc gccagggtgg tgtgatgctc gaactgcacc acctgcaggt agtacatgcg
    10201 cgccaccagc accgaggtca gcaacaaaac caccacagca ccgaccaccg cgcgtttgcg
    10261 gatcaggcgg ccgtctttct cgtggtcttt gaggcggatt ggttgcggca tcggtggctg
    10321 ctgattactg gctgattatt tgtggtaagg gtggccggac aacacggtcc aggcacgata
    10381 catctgttcg ccgatcagaa tccgcaccaa ggggtgcggc aaggtcagcg gcgacaacga
    10441 ccagcgctgt tcactgcgcg cttgcacctc aggtgccagc ccttccgggc cgccgaccat
    10501 caggttgacg gtgcgtgcat ccaggcgcca cttatcgagc tcgactgcca gctgctcggt
    10561 gctccaaggt cgcccttcga cttccagggt cactatgcgt tcccctggct gaaccttggc
    10621 cagcatggcc tcgccttcct ggcggatcat ccgcgccaca tcggcattct tgccgcgggt
    10681 ggtcagcgga atttccacca gttccaggga cagttcgctg ggcatgcgct tggcgtattc
    10741 ctgccagccc tcttccaccc agcgcggcat ccgcgagccg acggcgatca gtttgatccg
    10801 caccgctgcg cgtccttatt cctgttctgg gcccggctcg gcgctgaact gcgcacggct
    10861 ctgctcggca ccctgccaca gacgctcaag gtcatagaac tggcgcgcgg tcggcagcat
    10921 cacatgaacg acgatgtcgc cgaggtcgag cagtgcccac tcgccagtgt ccatgccttc
    10981 agtgccaatc gggcgcacgc cctgttcctt aaccttctcc agcacgttgt cgaccagcga
    11041 cttgacgtga cggctggagg tgccactggc gatcagcatg aaatcggtga tgctggtctt
    11101 gccgcgaaca tcgatggtgg tgatgtcttg cgccttgatc tcttccaggg ctgtgatggc
    11161 cagtttgacc aactcttcgc tctgcatttt tgccttagtc ataagtaact catttgcctc
    11221 gtgttcaatt cgcgccacgg tacagtccgt gggcatggat ataggccagt accgcgtctg
    11281 gcaccagaaa acgtgccgac tttccgctcg ccagtagcga acggatctgg gtagccgaca
    11341 ccgccagggg tgtctgccag ataaaagcaa tctgcccact cggcccactt agggccgaag
    11401 ggtcactcac actgcgcgcc gccaacagat tacgcagcgc ctcgggcgct tcactgtcgg
    11461 catccggccg ctgcaacacc agaatatggc agtgctgcag caattcatcc cagcgctgcc
    11521 agctgggtaa accacagaag gcatcccagc ccagcagcag aaacagctga tcgtccgccg
    11581 ccaactcgct gcgcaacgac tccagggtgt cgatgctgta ggacggctta tcgcgctgca
    11641 gctcgcgatc atccacctgc aacaccccgg catcggctac cgccaacctg accatggcca
    11701 ggcgatccac cgcgctgacc tgcggcgtac tgcgatgcgg cggtcgtgcg ctggggatca
    11761 ggcgcagctg atcaagctgc aacgcctcgg cgacttccac ggcgctgcgc aaatgaccga
    11821 tatgcaccgg gtcaaaagtg ccaccgagca agccaatacg ccgggccatc aggtacgccc
    11881 catcaggtac gaatgtgtcc gtcgccgaac accacatact tctcactggt cagcccatcc
    11941 aggccaaccg gaccgcgggc gtgcagtttg tcggtggaaa taccgatctc cgcgcccagg
    12001 ccatattcaa aaccatcggc aaagcgggtc gacgcgttga ccatcaccga ggcggaatcc
    12061 acttcggtga tgaaacgccg cgcatcacta aagttctcgg tgatgatcgc gtcggtgtgc
    12121 tgcgaaccgt aatggttgat gtgctcgatg gcggcatcca gcgaatcgac aatgcgaatc
    12181 gccaggatcg gcgcgttgta ttcggcaaac caatcgtctt cgctggcttc cagcacgtca
    12241 ctgcccagta gcgtgcgggt cgcggcgtca ccacgcagtt caacaccttt gtcgcggtag
    12301 atcgcagcca gcggtggcag caccttgtca gccacggcgg catgcaccag cagggtttcc
    12361 atggcgttgc atggcgagta gcgctgggtc ttggcgttat cggcaacgcg aatagccttg
    12421 tcgaggtcgg cggcgacatc gatatagacg tggcagacgc cgtccagatg cttgatcacc
    12481 ggaaccttgg cgtcacggct gatgcgctcg atcaggccct tgccgccacg cggcacgatc
    12541 acatcgacaa attccggcat ggtgatcagc gcaccaacgg cggcgcgatc ggtagtttcc
    12601 accacctgca ccgcgctggc aggcaagccg gcttcggcca gaccaagctg gatgcagcgg
    12661 gcaatcgcct gattggaatg aatcgcctcg gagccgccac gcaggatggt cgcgttaccg
    12721 gatttcaggc acaggctagc ggcgtcgatg gtcacgttcg gccgcgactc atagatgatg
    12781 ccgatcacgc cgagcggcac tcgcatcttg ccgacctgaa ttcccgacgg caggtaacgc
    12841 atgccctgga tctcgccgat tgggtcgggc agcgtggcga cctggcgcaa accttcaatc
    12901 atcgagtcga tcaccgccgg cgtcagcgcc agacggtcaa ccatggccgg ctccagacca
    12961 cccgcgcgag cggcggccag atccagctcg ttggcggcag tcaaatcgct gcgggccgca
    13021 tccagcgcgg ccgcggcggc ctgcagggca cgatttttct gcgcggtact ggcgcgcgcc
    13081 agcacgcgcg aagcgctgcg ggcggcctgt cccaggcggg tcatatagtc aagcacggac
    13141 tcagtcatgg tcttggcagg tctggcggtt gaaaaaagtc gctgagtata acggtcgcgc
    13201 cgct
//
LOCUS       DASRUM010000296         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_141314, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000296 DASRUM010000000
VERSION     DASRUM010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141314"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..78)
                     /locus_tag="VFF22_20030"
     CDS             complement(<1..78)
                     /locus_tag="VFF22_20030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX19144.1"
                     /translation="MSRRRVVVTGMGMLSPLGNDVPSSWQ"
     gene            complement(186..419)
                     /gene="acpP"
                     /locus_tag="VFF22_20035"
     CDS             complement(186..419)
                     /gene="acpP"
                     /locus_tag="VFF22_20035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZX19145.1"
                     /translation="MSTIEERVKKIVAEQLGVKEEEVTNSASFVEALGADSLDTVELV
                     MALEEEFETEIPDEQAEKITTVQEAIDYVTAHA"
     gene            complement(618..1361)
                     /gene="fabG"
                     /locus_tag="VFF22_20040"
     CDS             complement(618..1361)
                     /gene="fabG"
                     /locus_tag="VFF22_20040"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HZX19146.1"
                     /translation="MSLQGKVALVTGASRGIGQAIALELGRQGAVVIGTATSASGAER
                     IAETLKEHGIEGAGLVLDVSDSESVASTLEHIQQHLGQPLILVNNAGITRDNLMLRMK
                     DDEWFDVINTNLSSLYRLTKAVLRGMTKARYGRIINIGSVVGAMGNGGQVNYAASKAG
                     LEGFGRALAREVGSRAITVNAVAPGFIDTDMTRELPEAQRDALLTQIPLGRLGQAEEI
                     AKVVAFLASDGAAYVTGATIPVNGGMYMS"
     gene            complement(1377..2315)
                     /gene="fabD"
                     /locus_tag="VFF22_20045"
     CDS             complement(1377..2315)
                     /gene="fabD"
                     /locus_tag="VFF22_20045"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009042850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZX19147.1"
                     /translation="MSASLAFVFPGQGSQSLSMLAEHGAQRPLILETFAEASEALGYD
                     LWALTQQGPEEQLNQTDKTQPAILTASVALWRAWLAESAARPAFVAGHSLGEYSALVA
                     AGSLSLAAAVKLVEHRGQLMQQAVPAGQGGMAAILGLEDADVLAACAEAAQGEVVSAV
                     NFNAPGQVVIAGGAAAVERAIEACKARGAKRAMPLPVSVPSHCALMRPAAERFAEAVA
                     AIDWQAPQIALVQNVSAAAVTDLEGLKRDLLAQLYSPVRWVESMVYLSAQGVTDLVEC
                     GPGKVLSGLNKRCVKGINTHNLDTPDAFAAANAALV"
BASE COUNT          520 a          760 c          639 g          487 t
ORIGIN      
        1 ctgccagcta ctcggcacat cgttacccaa tggcgacagc atgcccatac cggtaaccac
       61 gacgcgtcta cgcgacacag caatctcctt ttctagttat caacgcctgg aaagcgctta
      121 aagaaaagcc gcacgcctga taaagggagt gcggcttttc cgagtcggga tccgacgatt
      181 acgttttacg cgtgagcggt aacgtagtcg atcgcttcct gaacggtggt gatcttctcg
      241 gcttgttcgt ccgggatctc ggtttcgaac tcttcttcga gagccatcac cagctcaacg
      301 gtgtcaagag agtcggcacc cagggcttca acgaaggaag cgctgttggt tacttcctct
      361 tctttgacac ccagttgctc ggcaacgatt ttcttgacgc gttcttcgat ggtgctcata
      421 cgttgttctc actcctaatg ggacaaatcc aggccactgt atctgcggcc aagtgtatag
      481 aaagggtttt tagcatttca agctgaatgc cggggtgccc ccaagaacac ttcaacgatc
      541 tgtctataaa cctgttgcag ctttataacg gattttagac agcatctatg acagttttct
      601 gaaggcatcc gtcacattta gctcatatac atcccgccgt tcacagggat agtagcccct
      661 gtgacgtaag ccgcgccatc ggaagcgagg aaagcgacca cttttgcgat ctcttccgcc
      721 tgccccaaac ggcccaacgg aatctgtgtc agcagcgcat cacgctgcgc ttccggcaat
      781 tcacgggtca tatcggtatc gataaaaccc ggcgccacgg cgttcacggt aattgcccgc
      841 gaacccactt cacgcgccag tgcacggcca aaaccttcaa ggcctgcctt ggaggccgcg
      901 tagttaacct gcccgccgtt gcccatggca cctaccaccg aaccgatatt gatgatccgg
      961 ccgtagcgcg ccttggtcat gccacgcagc accgccttgg tcagccggta caggctgctc
     1021 aggttggtgt tgatgacatc aaaccactcg tcatctttca tccgcagcat caggttatcg
     1081 cgggtgatgc cggcgttatt gaccagaatc aacggctgac caagatgttg ctggatatgt
     1141 tcgagggtgc tggcaaccga ttcgctgtcg ctaacatcca gcaccaggcc tgcgccctcg
     1201 atgccatgtt ccttcagtgt ttcagcaata cgctcggcac ccgaagcgga cgtggcggta
     1261 ccaataacca ccgcgccctg acgacccagc tccagggcaa tcgcctggcc aatgccacgg
     1321 ctcgcgccag tgaccaatgc aactttacct tgcaggctca taacgtttct ccttaatcag
     1381 accagggccg cgttcgcagc agcgaaggcg tccggggtgt ccaggttatg ggtattgatg
     1441 cctttgacgc agcgcttgtt caaaccagac aacaccttgc ccggcccgca ctcaaccaga
     1501 tcagtaacgc cttgcgcaga caggtagacc attgactcga cccaacgcac cgggctgtac
     1561 agctgagcca ataggtcgcg cttgagccct tccagatcag tcactgctgc ggcactgacg
     1621 ttctgcacca gggcaatttg cggggcctgc cagtcaattg ccgctactgc ttcggcaaag
     1681 cgctcagccg cagggcgcat cagcgcgcag tgcgacggca cgctaaccgg cagcggcata
     1741 gcgcgcttgg cgccacgtgc cttgcacgcc tcaatggcgc gctcaacggc agcagcgccg
     1801 ccggcgatca ccacctggcc cggcgcattg aaatttaccg cactgaccac ttcaccctgc
     1861 gcagcttcgg cgcaggcagc caatacatca gcatcttcca ggccgagaat cgctgccata
     1921 ccgccctgcc cagcaggaac cgcctgctgc atcagctgac cgcgatgctc caccagcttg
     1981 accgcagcag ccagcgacaa gctgcccgct gcgaccagcg ccgagtactc acctaagctg
     2041 tgcccggcga caaacgccgg acgcgcggcg ctctcagcaa gccaggcacg ccacagcgca
     2101 acagaggcgg tgagaatcgc cggctgagtc ttgtcggttt gattgagttg ttcttcaggc
     2161 ccttgctggg tcaacgccca gaggtcataa ccgagggctt cggaagcctc ggcgaaagtt
     2221 tcgaggatca gcggccgctg cgcaccatgc tcggcgagca tgctcagcga ctgggaaccc
     2281 tgacccggaa agacgaatgc gagagatgca gacatggaaa cctgttccta atgatcgtga
     2341 tcgtcggtaa tacgcgccca gcctagccaa gcgcaagaaa ctgacagttg gatgacaggc
     2401 tgcccg
//
LOCUS       DASRUM010000297         3416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1152853, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000297 DASRUM010000000
VERSION     DASRUM010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152853"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="VFF22_20050"
     CDS             complement(<1..1304)
                     /locus_tag="VFF22_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZX19148.1"
                     /translation="MTENFWKDKYPVGIAAEINPDQYPNIQAVLKESCQRFADKPAFS
                     NMGKTITYGELYELSGIFAAYLQQNTDLQPGDRIAVQLPNVLQYPVVVFGALRAGLVV
                     VNTNPLYTARELEHQFNDSGAKALVSLANMAHLVEQVLPKTGIKTVIITEVGDMLSPL
                     KRLLVNTVVKHVKKMVPAYNLPQAVKFTAAMAKGRGQSAKEAAPSNGEIAVLQYTGGT
                     TGVAKGAMLTHRNLIANMLQVKALMGSEMSEGSEVLIAPLPLYHIYAFTFHCMAMMLI
                     GGHNVLITNPRDLPAMTKDLAKYKFTGFVGLNTLFVALCNNEDFRKLDFSTLKATFSG
                     GMALQLATAERWKQVTGCSICEGFGMTETSPVASVNPFSNIQIGTIGIPVPSTQCKVI
                     DDEGVEQPLGSIGELCVKGPQVMKGYWQRQEATDEILDAEGW"
     gene            complement(1611..3299)
                     /gene="fadD2"
                     /locus_tag="VFF22_20055"
     CDS             complement(1611..3299)
                     /gene="fadD2"
                     /locus_tag="VFF22_20055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD2"
                     /protein_id="HZX19149.1"
                     /translation="MQPDFWSDKRPAGVPNDIDLGTYKSVIEVFERSCKKFADRPAFS
                     NLGVTLTYAELDRLSAAFAAYLQKHTDLQPGERIAVQMPNVLQYPIAVFGAMRAGLIV
                     VNTNPLYTAREMRHQFKDAGVRALVYLNMFGKLVQDVLPDTEIDYLIEAKMGDMLPSL
                     KGWLVNTVVKKVKKMVPDYHLPQAVAFKDALKQGHGHALKPVKVGQEDIAVLQYTGGT
                     TGVAKGAMLTHGNLVANMLQVDACLSQLGNDGTPLMKQGQEIMIAPLPLYHIYAFTAN
                     CMCMMVNGNHNVLITNPRDIPGFVKELGKWQFSALLGLNTLFVALMDNPEFQKLDFSK
                     LKVTNSGGTALVKATAERWQAMTGCTVVEGYGLTETSPVASTNPYGDKARLGTVGIPV
                     PGTAFKVIDDDGVEQPLGERGELCIKGPQVMKGYWNREEATAEVLDAEGWFKSGDVAV
                     IDPDGFVRIVDRKKDMIIVSGFNVYPNEIEDVVMAHPKVASCAAIGVPDEKSGEAVKL
                     FVVARDSSLNADELKAYCKENFTGYKVPKQIVFRDSLPMTPVGKILRRELRDIA"
BASE COUNT          669 a         1000 c         1008 g          739 t
ORIGIN      
        1 aaccagcctt cggcgtcgag gatttcgtcg gtggcttcct ggcgctgcca gtagcccttc
       61 atcacctgcg ggcctttgac gcacagctca ccaatcgaac ccagcggctg ctcgacgcct
      121 tcgtcgtcga tcactttgca ctgggtcgac ggtaccggaa taccgatggt gccgatttgg
      181 atattgctga acgggttgac cgaggccacc gggctggttt cggtcatgcc gaagccttcg
      241 cagatcgagc aaccggtcac ctgcttccaa cgctcggcag tcgccagttg cagcgccata
      301 ccgccggaga acgtggcttt gagggtagag aagtccagct tgcggaagtc ctcgttgttg
      361 cacagggcaa cgaacagggt gttgaggccg acaaaaccgg tgaacttgta cttggccagg
      421 tccttggtca tcgccggcag atcacgcggg ttggtgatca gcacgttgtg cccaccaatc
      481 agcatcattg ccatgcaatg gaaggtgaag gcgtaaatgt ggtacagcgg caatggcgcg
      541 atcagcactt cgctgccttc gctcatttcc gagcccatca gcgccttgac ctgcagcatg
      601 ttggcgatca ggttgcgatg ggtcagcatc gcgcccttgg ccacgccggt ggtgccgccg
      661 gtgtattgca gcacggcgat ttcgccattg ctcggcgcag cttccttggc cgactggcca
      721 cggcctttgg ccatggcagc ggtgaacttg acggcttgcg gcaggttgta ggccggtacc
      781 atcttcttca cgtgcttgac gaccgtattg accagcaggc gcttgagcgg cgagagcatg
      841 tcaccgactt cggtgatgat gaccgtcttg atcccggttt tcggcagtac ttgctcaacc
      901 aaatgggcca tgttggccag gctgaccagg gctttggcgc cggaatcgtt gaattggtgt
      961 tccagctcgc gcgcggtgta cagcggattg gtgttgacca ccaccaaacc ggcgcgcagt
     1021 gcaccgaaga ctacaaccgg gtactgcagc acgttgggca attgcacggc gatgcggtcg
     1081 ccgggctgca ggtcggtgtt ctgctgcaag taggcggcaa agatgccgga caactcgtac
     1141 agttcaccgt aggtaatggt tttacccata ttgctgaaag ctggcttgtc ggcgaagcgt
     1201 tggcaggact ctttcagtac cgcctggatg ttcgggtact ggtcaggatt gatttcggca
     1261 gcaatcccaa cgggatactt atccttccaa aagttttcgg tcatggaagc ccactcctca
     1321 gcaacagctt gatcttcacc gcgcatagac ggttgtttat tgtagatttg cgtgttttct
     1381 gcgatttgaa cggcaaaaaa gccgcccgag agtagcagct ttgccaaaca gcgactagca
     1441 ccaataagcg ccgcagcagt cacaaaaacg accaataatc aatatttggt cacttagatg
     1501 atttcgctga aacaggcggg ctacaggcgt tgcacagagc aggaagggat tttcagcgca
     1561 tccacagtta aggcggcggt atgcgctgaa acggtaactg ccgtcgagga tcaggcgata
     1621 tcgcgcagct cgcgacgcag aatcttaccg actggcgtca tcggcagtga atcacggaag
     1681 acgatctgct tgggcacctt gtagccagtg aagttttcct tgcagtaggc cttcagctca
     1741 tcggcgttga ggctgctgtc gcgggccacg acgaacagct tgaccgcttc cccggacttc
     1801 tcgtccggca caccgatggc cgcgcagcta gcgactttcg ggtgggccat gaccacatct
     1861 tcgatttcat tgggatacac gttgaaaccg gagacgataa tcatgtcctt cttgcggtcg
     1921 acgatgcgca cgaaaccgtc cgggtcgatc accgccacgt caccactctt gaaccagccc
     1981 tcggcgtcca gcacctcggc ggtggcttct tcgcggttcc agtagccctt catcacctgc
     2041 ggacctttga tgcacagctc gccacgctcg cccagcggct gctcgacgcc atcatcatcg
     2101 atgaccttga acgccgtgcc cggcaccgga atgcccacag tgcccagacg ggccttgtcg
     2161 ccgtagggat tggtgctagc caccggcgag gtctcggtca ggccatagcc ttcgaccaca
     2221 gtacaacccg tcatcgcctg ccagcgctcg gctgtggcct tgaccagcgc ggtgccgccg
     2281 gagttggtga ccttgagttt ggagaaatcc agcttctgga actcggggtt gtccatcaat
     2341 gccacgaaca gcgtgttgag gcccagcagc gcggagaact gccacttgcc cagttccttg
     2401 acgaagcctg ggatgtcacg cgggttggtg atcagcacgt tgtggttgcc gttgaccatc
     2461 atgcacatgc agttcgcggt gaaggcatag atatggtaga gcggcagcgg cgcaatcatg
     2521 atttcctggc cctgcttcat cagcggggtg ccatcgttgc ccagctgcga caggcaggca
     2581 tcgacctgca gcatgttggc caccaggttg ccgtgagtga gcatcgcgcc cttggccaca
     2641 ccggtagtgc caccggtgta ttgcagcacg gcgatgtctt cctgaccgac cttgaccggc
     2701 ttcagcgcgt ggccatggcc ctgcttgagc gcatccttga acgccaccgc ctgcggcagg
     2761 tggtaatcag ggaccatctt cttgactttc ttgaccacgg tgttgaccag ccagcctttc
     2821 aggctcggca gcatgtcacc catcttggct tcgatcaggt agtcgatctc ggtatccggc
     2881 agcacatcct gcaccagctt gccgaacatg ttcaggtaca ccagcgcgcg cacgccggca
     2941 tccttgaact gatggcgcat ctcgcgggcg gtgtacagcg ggttggtgtt aaccacaatc
     3001 agcccggcgc gcatggcgcc gaacacggca attggatact gcagcacgtt gggcatctgc
     3061 acggcgatgc gctcgcccgg ctgcagatcg gtgtgttttt gcaggtaggc ggcaaaggcc
     3121 gccgagagcc gatccagctc ggcataagtc agggtcacgc ccaggttgct gaaggcgggc
     3181 cggtcagcga acttcttgca cgagcgctcg aacacctcga taaccgactt gtaagtcccc
     3241 aagtcgatgt cattgggtac gccggccggg cgtttgtcgc tccagaaatc aggctgcatt
     3301 atttttatcc tcttgccctg agatggtcta atccgcgctg cggagttggc tggaagttaa
     3361 cagctaacgc caaacaggca aatagccgag cagcgtcatt gacgcactga atcttg
//
LOCUS       DASRUM010000298         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_494133, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000298 DASRUM010000000
VERSION     DASRUM010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494133"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..425)
                     /locus_tag="VFF22_20060"
     CDS             complement(108..425)
                     /locus_tag="VFF22_20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HZX19150.1"
                     /translation="MKPALYSSRTADKFVVRLPDGMRERIADVARNHHRSMNSEIIAR
                     LEQSMLQESSLGDDLNMRLDSPDLSLHERELLQRFRQLSRRQQNALVALIAHDAEMAA
                     EEA"
     gene            818..1654
                     /gene="phnC"
                     /locus_tag="VFF22_20065"
     CDS             818..1654
                     /gene="phnC"
                     /locus_tag="VFF22_20065"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HZX19151.1"
                     /translation="MSAVIRVESLNKTFGRKQALFDLALSVETGEMAALIGASGSGKS
                     TLLRHVAGLACCDRSGGGSIQVLGREVQADGRLNGEVRRLRADIGYIFQQFNLVGRLS
                     VLQNVLLGCLGRMPRWRGTLGLFNAEEKQRAMQALARVGLADLAQQRASTLSGGQQQR
                     VAIARALCQRAKVILADEPIASLDPESARKVMQILADINREDGTTVVVTLHQVDYAMR
                     YCQRAVALKAGRIHYDGAAAELHPNFLNDLYGAELIVEPQSDEKPRRARKPKVPLALA
                     KA"
     gene            1711..>2062
                     /locus_tag="VFF22_20070"
     CDS             1711..>2062
                     /locus_tag="VFF22_20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HZX19152.1"
                     /translation="MFKRISRVLAASTLLAGSVLGAAQAAEQEINFGIISTESSQNLK
                     SMWDPFLADMSEQTGLKIKAFFAPDYAGIIQGMRFDKVDMAWYGNKAAMEAVDRANGQ
                     IFAQTVAANGTQGYY"
BASE COUNT          434 a          596 c          610 g          422 t
ORIGIN      
        1 ctgattaccg cgctcaataa aaaaccgccg aaaggcggtt ttttattggc agaaaaatac
       61 acagcaaaaa aaccggccta ggccggttct ttcgttgtgc agcgcagtca ggcttcttcg
      121 gcagccatct cggcgtcgtg agcaatcaat gccaccaggg cattctgctg acggcgtgac
      181 agttgacgga agcgttgcag cagctcgcgc tcgtgcaagg acagatctgg gctgtccaag
      241 cgcatgttga ggtcatcacc caaagagctt tcctggagca tgctttgctc aaggcgagca
      301 atgatctccg agttcatgct gcggtggtgg ttgcgtgcga catccgcaat acgttcgcgc
      361 atgccatcgg gcagacgaac tacgaatttg tcagcggtgc ggctggaata gagtgctggt
      421 ttcatagggc gcgtacatta aaccggttag tttaggaaag cgatactggc aatgggctat
      481 aggattgtca ccggtttgac cacctatagc actaaccgtt cagccagaaa gccgcttaga
      541 tcgtatgtga ctctacagtc gcgaggtcgg taaattgacg ccaatcgcgt gtcgcatttt
      601 gcgtcgggta aaggcgttat gccagcacca gtcatcagaa atgcgccgtg ctgtgcaaaa
      661 agccaggaaa tcttcccgcc catagcaaaa acttcaagca atggtcacgt aagtgtcacg
      721 gcccattaat cgctctgaca caggatttgc ctaccttggt ctacaccaga aggcggcttc
      781 acgccgtggt gtaaccgcta ataaaaggag gcattccatg agcgcagtga tccgggtcga
      841 gagcctgaac aaaacgttcg gccgcaagca ggcgctgttt gatctggcac tgtccgtaga
      901 aaccggtgaa atggcggcgc tgatcggagc ctcgggttcc ggcaagtcga ccttgctgcg
      961 tcatgttgcg ggtctggcct gctgcgatcg cagcggcggt ggcagcattc aggtgctggg
     1021 ccgtgaagtg caggctgatg gtcgcctgaa tggcgaagtc cgtcgcttgc gtgccgacat
     1081 cggctacatc tttcagcagt tcaatctggt cggccgcctt agcgtgctgc agaacgtgct
     1141 gctcggttgc ctggggcgca tgccgcgctg gcgaggcacc ctcggcctgt tcaatgccga
     1201 agaaaagcag cgtgccatgc aggccctggc ccgtgtcggc ctggcggatc tggcgcagca
     1261 gcgcgcctcg actctgtcag gcggccaaca gcagcgagtg gcgattgccc gtgccctgtg
     1321 ccagcgcgcc aaggtgattc tagccgacga gccgattgcc tcgctcgacc ccgaatcagc
     1381 gcgcaaggtc atgcagattc tggccgacat caaccgcgaa gacggcacca ccgtggtggt
     1441 caccctgcat caggttgact acgccatgcg ctattgccag cgcgccgtgg cgttgaaagc
     1501 agggcgtatt cattacgacg gcgccgccgc cgagctgcac cccaacttcc ttaacgatct
     1561 ttacggcgcc gagctgatag tcgagccgca atccgacgag aaaccgcgcc gtgcgcgcaa
     1621 gccgaaagtc ccgctggctc tggccaaggc ctgagccgct tgcctgttat gccaaccctt
     1681 gtatgtccat ctgcactagg agtgctttgc atgttcaaac gtatcagtcg cgttctcgcc
     1741 gcgtccacgc tgctggccgg ttcggttctg ggcgccgccc aagccgctga gcaggaaatc
     1801 aatttcggca tcatctccac cgagtcgtcg caaaacctga aaagcatgtg ggaccccttc
     1861 ctggcggaca tgagcgagca gaccggcctg aagatcaaag ccttcttcgc ccctgactac
     1921 gccgggatca tccagggcat gcgtttcgac aaggttgaca tggcttggta cggcaacaag
     1981 gccgcgatgg aagcggtgga tcgtgccaat gggcagatct tcgcccagac cgtcgcggcg
     2041 aacggcactc agggctatta ca
//
LOCUS       DASRUM010000299        39849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_985899, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000299 DASRUM010000000
VERSION     DASRUM010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 39849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39849
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985899"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(367..1311)
                     /locus_tag="VFF22_20075"
     CDS             complement(367..1311)
                     /locus_tag="VFF22_20075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX19153.1"
                     /translation="MLLDFHDQQLKYLFLSHQLGSMRAAADELGIAPSSISRQIAKLE
                     AELDIDLVEQGTHRIRLTEAGQVAIDYYRARARQHEALLNQLSELRGRFLGTTIIAVG
                     EGLLGSQAIVELQNFFKQYPDAHTEIIIAPSFEVQRMIQDDEAHIGLAFAPTSTVTLR
                     SLYAFEQPLRAIVSKNSPLAKKHVLTLTDLANTPLVLPGPKYRVRELVTQACKDEAFT
                     IEPVVTANSLQVILDLVRAGIASTLLPELPLVSELTSGLFKAIPLACEAMNSTEMRII
                     VRPSRKLSPVSHELIRHLARAISLMRRGDGRIAKSVLA"
     gene            complement(1935..3119)
                     /locus_tag="VFF22_20080"
     CDS             complement(1935..3119)
                     /locus_tag="VFF22_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZX19154.1"
                     /translation="MSTLTLPECTVSVKQLGEGGWQAAMDVLIEQVRLRRLEFDQGKH
                     VPVDVINLMKKAGIFRASTPACFGGEPLPPAELLRRVERISEVDGSAGWVSAFGSANT
                     YLARLPYETQKLLYATGPDQVFAGGLFPPHYGEAVEGGWILNGRWKFASGCKGADWMG
                     VGIKVDSPDGKSPPSVRMAVMPATEVEIVESWDVVGMQGTGSHDLRLENTFVAHEWTC
                     QRELPSPIDEPLYRYPALAYQAQVHAVVNIGLARAALDTVIEMGGGAKIIPGASRLAD
                     RAYYRTSIAQCEAKLRSARAFFYETAEQAWDMLISKGELTRELHSLVLLSAAHAAHTS
                     TDIVQEAYRIAGIGVIQKSHRLQAIARDSMVITQHAALSLGTFDAAGSIFVGVEPVIP
                     YV"
     gene            complement(3170..3706)
                     /locus_tag="VFF22_20085"
     CDS             complement(3170..3706)
                     /locus_tag="VFF22_20085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003232755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HZX19155.1"
                     /translation="MTTITTNASNTFRQAMSLLAAAVNIVSTDGPDGRFGVTASAICS
                     VTDTPATLLVCLNQKGAVHDAALRNKTLCINILHGDHESLARTFAGMEYVPHEERFNP
                     DAWVEGKSGLPVLRDALASLEGRVVDVKEVGTHSVLFVEIDHINVREDGECLTYFGRK
                     FHRLGLSPAPSLNTASAG"
     gene            complement(3798..4274)
                     /locus_tag="VFF22_20090"
     CDS             complement(3798..4274)
                     /locus_tag="VFF22_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003173908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HZX19156.1"
                     /translation="MNLQLLNQVTAFIWQEADMLDHSEFEAWLALWAPKGTYIIPIDP
                     DETDLENTLNYAYDNHHMRELRVKRLTSGESISTSPRARTVRTLSRFRLLSDADGVVT
                     VRCAQNLREFRKDLLKHYTADITFELLRDGDSFTIQRKLIKLINSTDTLAGIGYIL"
     gene            complement(4293..5570)
                     /locus_tag="VFF22_20095"
     CDS             complement(4293..5570)
                     /locus_tag="VFF22_20095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003314948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZX19157.1"
                     /translation="MSELINAVNLVDSPSELVQPDRVHTSLYTDAKLFDVELDKVFYS
                     TWVWVAHASEIPDAGSYKTTYVGKQPVIVVRDRKKAVHVLLNRCRHRAATVCEGKKGK
                     TNSFVCPYHGWSYALDGSLRGIPSPQSYADLLDKAEHPLVSLRVEAYNGMIFATFKDD
                     IEPLVDFLGPAKKWIDLFMKQGAGYPIKVAGEHRFRFPGNWKIQLENTTDAYHFPLVH
                     KSFLSSVDKQTEELFDFVEGPGYVEDLGNGHSVMVMIPELVDLEEELMERPIPERFEE
                     LAQELRDEGHDELSVRRITRAVGGSGFNLNLFPNVACSMAFFRVLQPIAVDETEIHHS
                     VITMDGGPATANRYRLRLHEHFQGPMGFGTPDDSEAWERVQKGSSAGKDLWIMLNRGL
                     AGETKSDDGLVSDVSAETGMRAAYQQWKKMMSV"
     gene            complement(6507..7832)
                     /locus_tag="VFF22_20100"
     CDS             complement(6507..7832)
                     /locus_tag="VFF22_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008033879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HZX19158.1"
                     /translation="MHKSSFFRISILAALIAGSTQASAYELYADEDTHLNANLEATFG
                     FFNSQQNYSNSGSKSEGSSTWREGYIKYGISGDQGLAGAGTAYGAFSLLSSATWGDGD
                     ATGFSNGSERTTKIEDAYAGWRSGELFEALGKDGVDVSFGRQNIAVGDGFLIYGDGTN
                     LGKGLADGEFNRGGSTYLGARKNFDKTAVLRLGGQEGWRSDLMWLKSDNRGQAKTEMY
                     VATLEHVAEPGTVGLTYINTIDVDEKYASPTQLERDGMKTYSLRAAGNAGVENLFLSG
                     EYAKQDKPHTATEDAWYLEAGWTFADVAWSPSVSYRYSRFSEGYDPLFYGFSRAFGTW
                     FQGEVAGSYAGPFSTNTRVQHLSLKVSPLENLTISALYFDFDTIDRSLGNPSGQEIDI
                     LAEWGVTENLMIIPLIGLYQPEKSAAQGGTQLGNDDLNLYTQLVFATSF"
     gene            complement(8150..9553)
                     /locus_tag="VFF22_20105"
     CDS             complement(8150..9553)
                     /locus_tag="VFF22_20105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZX19159.1"
                     /translation="MIESTNISSSNLTATSPNPQLSKKFGLFTMMAVCIGLVVAQGAM
                     ISTAQGVGFGAMTFLAAMVAALAISQCNAMSFAELSLMFPQKGTLATYTQKAMGHVPA
                     IVAVFAGYIIVSVLAVPVEMFMIDIILAQLLPGFVPDKAISLFILVGLTLTNLMGTDV
                     FARIQNLLVLVMVSTIALVSCVAITGVAQPHPELTGTPVDWGFSSVTDFGFIGFIALS
                     MWCFVGIEYICSLISEVKDPHKNVPRAMHLSLFVIFFLFVGFGYGITFYMDSETLVSA
                     GMPYVDFIMAVFGSSTGLLITAVVALAATSSSINTVLAAVPRMMHGMAEQGQLLPIFK
                     KVNRFNVPWVATAVLSISIVIPYLSLDMDSIILFVIAASTSYLIAYLIAHLNVVILRK
                     RMPDHPRPYRSPFFPVPQIFAIVAIVYVAINNSPSPELTSSVYAITGGMLLFITICSV
                     LWVKFYMKRGIFEPDMS"
     gene            complement(9600..10892)
                     /locus_tag="VFF22_20110"
     CDS             complement(9600..10892)
                     /locus_tag="VFF22_20110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009408094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZX19160.1"
                     /translation="MTKTLESVRTTDQLPKSTTVVIIGGGIIGVTAALTLAERNIPVV
                     LLEKGHIAGEQSSRNLGWVRKTNRHADDVPLALAADRLWAGIPERIGREVGYQQSGIM
                     FLAENQERLALHESWLKSVESLSLDSQILSAREVNERVPRGKGEWAGALYTPSDGKAE
                     PHLATSALAQAAIEKGAILIQNCAVRTLSTSAGKVSGVVTEHGEIRCDQVLLAGGAWS
                     RRFLGNVGVSYPTLPLICSVLRTTPMDGPTDIAVGGPDFSFRKHIDGGYIITQRAALD
                     AKLTLDHLLIGHRYFGLLRGQGSFLRVSLGKPFLQDLALKRRWRADQTSPFERVRTLD
                     PSVNEGLIQEALSNLRNAWPVFENARISEAWAGQIEITPDSNPVIDRIQSIPGLTIAT
                     GFSGHGFGTGPAAGQLAADLVANMTPLVDPAPYRFDRL"
     gene            complement(10942..11361)
                     /locus_tag="VFF22_20115"
     CDS             complement(10942..11361)
                     /locus_tag="VFF22_20115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017846980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HZX19161.1"
                     /translation="MTNIVKVNTDSKFEVLGSYSRAVAVDSWIYVSLTAGRNPDTKEI
                     PEDAVEQAEQVFRIIEKTLASLDASLADVVMSRVFVQSPENVNAVMGFIAQKFKGINP
                     ATTITCPPLGSTLYKVEVEVTAYRGASNLNKVNITLE"
     gene            complement(11401..12543)
                     /locus_tag="VFF22_20120"
     CDS             complement(11401..12543)
                     /locus_tag="VFF22_20120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018927864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyramine oxidase subunit B"
                     /protein_id="HZX19162.1"
                     /translation="MSKHNTKIDFIYLSEQDMIKAGVMDMAECVNTMEQMFILLQQGD
                     YRMAGANNDSHGSQITFPADSPFPNMPKPTPDRRLMAMPAYLGGDFQTCGVKWYGSNA
                     ANREKGLPRSILTFILNDIDTGAPLAYMSANLLSAYRTGAVPGVGARHLARKNSKIIG
                     LLGPGVMGKTSVTAFMSTCPEIDTINIKGRGEKNLNRFVTWVNEVFPQIKNVNVVDTV
                     EDVVRGADIITYCNSGDVGDPSTYPLVKREWVKPGAFLAMPAFCRLDDGMEQADVRKV
                     LDNTGLYDAWYEELPKPAHNLIPLVGMRFMDMVGEGKLSRNALEDLGDICAGLTPARQ
                     NDEEIIILSVGGMPVEDVAWATVVYRNALEKGIGVKLNLWDEPVLR"
     gene            13103..13456
                     /locus_tag="VFF22_20125"
                     /pseudo
     CDS             13103..13456
                     /locus_tag="VFF22_20125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_058159172.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(13537..14403)
                     /locus_tag="VFF22_20130"
     CDS             complement(13537..14403)
                     /locus_tag="VFF22_20130"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZX19163.1"
                     /translation="MTILSSCSVRGVTDKRIFNYIDTSLGQLHYAESGAGDVLILLHQ
                     TPRSLDEFAEIQPLLASRWRTIAMDMYGFGQSAKFAAPQTVEQYAQGVLALVDALNIE
                     KFSILGHHTGMFVASEVAAIAPGRVTAVVLSAGDIVDAAFREAMAALDVDVAETREDG
                     SHLMELWRKRLELYPPEHTDVLNRFIRDALAPGVTPLEGHLAVARYDMEDRVGLVTAP
                     VLVLAATDDPVTYSRTQVVVDAYCNSCFVSHAEIKGGGIPLMELKSKEVADTVHRFFD
                     DLECNVIGVKRD"
     gene            15112..15945
                     /locus_tag="VFF22_20135"
     CDS             15112..15945
                     /locus_tag="VFF22_20135"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX19164.1"
                     /translation="MSVPSLASPYLSMHSHQPWFVLQAAKEFSVSLSDNPLISHFYSF
                     ETKSSSPFMLSVPDGCIDIIFDCDDTNPSVSVHGTMLQAGSVEFRKGHRYVGVRFAPG
                     FVPDFLKLSAEELIEGSFDLLDVSVNAERVFEQVVREKNFVTQLTFLKQFFLTNIAPR
                     KSLKLTLPVVNMICQAQGNVCIKNLESLTGYTSRTLQRSFHNDLGMTPKMFCRIIRCQ
                     SAIYKIHHNNIGFSNLAIDLGFSDQPHFLREFKHFVSATPLDYKTRILKENFFSRIHF
                     Y"
     gene            complement(16160..17284)
                     /locus_tag="VFF22_20140"
     CDS             complement(16160..17284)
                     /locus_tag="VFF22_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003208862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZX19165.1"
                     /translation="MNPFVIQKSIGHQDGPTIAIKDSIDVSGWVTQAGSRALADNLPA
                     IKDADVVRLLLESGWQIVGKTVLHEFAFGVSGINDWAGTPINPQAKDLIPGGSSSGSA
                     VAVAAGAVYASIGTDTGGSVRMPAACCGVFGFKPTFARVSRAGAYPVHSTLDCIGPFA
                     REMAGIVDVMTAIDPSFMPATPRAEYRVGVVEVECDGIVSEAVYDALSATSWTCQAVK
                     LPSLAQAFNAAMVLINHETWAAFGQYTGQGLLGRDVEARLLAASRTTADEVTAAELVR
                     EQFCLEVDEQLKVFDALVMPSLPRLPPSVESVRAGASILDMTAFLRPFNLSGHPALTV
                     PVQCKGSEIKAGLQLIGPKHQDEQLCALGAVLERVLKVRR"
     gene            complement(17293..17796)
                     /locus_tag="VFF22_20145"
     CDS             complement(17293..17796)
                     /locus_tag="VFF22_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZX19166.1"
                     /translation="MPTIDLNDGFLQLHARLDRLESESAIRRLFTRYMQLCDVPCVQP
                     EGVAEALGGLFSPDAVWQGVGRLYTSKFGVLRGRAAIINMLMSYLPPSPHFRTNGHLL
                     GSEHIDVEGDQARGSWLMQQLSDYEEGSADLILARLDIHFIRINGVWLISQFQTERLL
                     SSPLTLA"
     gene            complement(18168..18776)
                     /locus_tag="VFF22_20150"
     CDS             complement(18168..18776)
                     /locus_tag="VFF22_20150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017278781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HZX19167.1"
                     /translation="MTIETRTLTKDSQGNEAGLPERFNLLAQLLHWTMAAAILAMLFI
                     GVAMMTSLTLRPELLDLHQPLGIAILLLVIVRLVNRLRHGAPALPADIPRWQVISANA
                     SHWLLYALMFSLPLLGWGVRSAGGWPVTMFSGLTLPPIAPTHPVWYAVLRDAHGLLAW
                     LLFAVVLMHLSAALMHAWVRRDGVFSSMTPGGLYRRYRRNGR"
     gene            complement(18773..19846)
                     /locus_tag="VFF22_20155"
     CDS             complement(18773..19846)
                     /locus_tag="VFF22_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017278782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HZX19168.1"
                     /translation="MKIGDIDLKRSWPALAGIGAIVGVLATAFAWTSDLLGQRVTNTT
                     FLEDTQHGFPAGYRRAHGKGMCFEGVFRSSGAAAKYSHARVFAQPETRAIGRFSIGTM
                     SPYAADNSTSTVSMAMMLTADNGQQWRMKMNNEPYFPTRDAPGFLEMMKATAPDPTTG
                     KANPQQLAAFLAEYPEARKYMEADAVKPWASSFAGATYYAIHAYFLVAEDGRRQAMRW
                     WLRPHATFTAWSDDERAGASHDALFKEVAQRLTKAPLHWDMVMQLAAPGDPVDDPSQP
                     WPETRQQVVAGTLEVQRVFDQTDGVCRDINFDPTLIPAGMALSNDPVLATRAGIYAHS
                     YNSRLHEIGYDKATDAVGKKTAQ"
     gene            complement(19986..20165)
                     /locus_tag="VFF22_20160"
     CDS             complement(19986..20165)
                     /locus_tag="VFF22_20160"
                     /inference="COORDINATES: protein motif:HMM:NF013089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HZX19169.1"
                     /translation="MFLRICIYGLAQALKGWPLGIIYAIWAGLGIVLATLVGVVVFKQ
                     RLDLIFQQSSASLLL"
     gene            20357..20923
                     /locus_tag="VFF22_20165"
     CDS             20357..20923
                     /locus_tag="VFF22_20165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZX19170.1"
                     /translation="MTSKANASRPPKQPDQVRKRLFHAATVLLSTGQPLSIGAIASAA
                     GVSKGAVQHHFGTKEQLLAAVFDLYMAQFNEALALEDASAPPALRYARMSLNATSYED
                     TDGWRAMVVATVIERGLAARWSERAEIERSLDQPPSANALLVRLAADGLWLSDLLSTY
                     KISNKEREELDVLMQELLTDPLINAIRP"
     gene            21301..22521
                     /locus_tag="VFF22_20170"
     CDS             21301..22521
                     /locus_tag="VFF22_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003173928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HZX19171.1"
                     /translation="MEKSHSGNPSLKFAIALGAIAALGPSAVDMYLPSLPEMAVEYST
                     SVASIQLTLTVFLLAMGLGQLLFGPVVDALGRRRPLMAGLLLFSLSALSAGAAPTLEW
                     LLVARFAQGIGSALTLVVIMSMVRDVTQGVQAAKLFGLLLTIQGVAPIIAPGLGGYID
                     AHYGWRAVMLVLAALGALVAVNSMAVLPETLPVQKREPLNMRQALRTYARIAQDSRFL
                     KPTLALSAVFFFLFAYVGGATFVYQNEYGLPTGQFGTLFGATGIAILIGAVVATRLVG
                     KYGLARMATTGAFCIFAGSVMAYGAAVSGSDLYWLAAGVAIALLGLGIAETSLMTLVM
                     SSQTTALGSTAALLGAFQLGISAFATPLTGWASSHGAIAWCAFLIVCAVLVGALTWVS
                     VRQGRAEHITALNH"
     gene            22538..23143
                     /locus_tag="VFF22_20175"
     CDS             22538..23143
                     /locus_tag="VFF22_20175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003232764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX19172.1"
                     /translation="MKVLSPSAGRICDIALVHFAERGYDASSLNDIAVVAGMRKPSLY
                     AHFKSKDDLFDAVLDLALVTESHYIEEMFASVPANNDEPGKLYTDSLSNRYESSAALR
                     FLLRTAFFPPSELKASVTRGFEAYLDRLRELFGASLESRYPALSGNEISMFQDAYIGV
                     IDSLHVELIYCSEGTYRRRLAALWRILGDSLSLAVGKVAHG"
     gene            23231..23866
                     /gene="gstA"
                     /locus_tag="VFF22_20180"
     CDS             23231..23866
                     /gene="gstA"
                     /locus_tag="VFF22_20180"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008003163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HZX19173.1"
                     /translation="MKLYYKPGACSLSPHIVANELGLNVEFIKVDLKDHVTEQGKNFY
                     KVNPHGYIPALELDNGEMLLEGVVIIQYLADQKPEIGLAPANGTFERYKLQEMLAFLS
                     TEIHKGFIPLFYAAPAGDYIEIARPKLEKRYAWINEHLSDKQFLMGDKFTVADTYLFA
                     VTGWAKASWLKSYEDTPLHLDSLHHLQGWYERVKSREAVQQSILEEGLDLN"
     gene            23926..25119
                     /locus_tag="VFF22_20185"
     CDS             23926..25119
                     /locus_tag="VFF22_20185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HZX19174.1"
                     /translation="MNLRAILILGALSAFGPLAVDFYLPGFPAIALAFGTDEKHVQLT
                     LASYFLGLSIGQLAYGPVADRFGRRIPLLFGASLFTLASLACAFAPNLEWLISARFVQ
                     ALGGCAGMVIARAIVSDKCDAVQSAKVFSQLMLVMGLAPILAPMLGGLLVNLYGWQSI
                     FISLTAFSALASLAVALGLPESMPATHPRLPLSGALTQYRRLLGDRIFLGHALTGGIA
                     MAGMFSYIAGSPFVFIKLYGVPPEHYGWLFGTNAAGFILVSQFNARLVGKKGPAFLLS
                     RVIWVYLAAALALLAIAGLRTEQLWPLLVPLFICIACLGSIAPNASACAMNGQWARAG
                     SASALFGCLQFSVAAAAAALVGVLHDGTALPMALVISSCGALAVTLAMLTRRLQHKRD
                     VALPG"
     gene            25224..25592
                     /locus_tag="VFF22_20190"
     CDS             25224..25592
                     /locus_tag="VFF22_20190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX19175.1"
                     /translation="MSNLTSSPLFRIDEFPDLYVKTTNPTVAVIVATPQLREINPSRA
                     SVQEKVHPNLNNRHSLMALANNYDNLSAGAQSAIGKRTQLFINGEFRNPLSGQYFPIV
                     DPSNGLELAEVAQADKQDAD"
     gene            25605..25787
                     /locus_tag="VFF22_20195"
                     /pseudo
     CDS             25605..25787
                     /locus_tag="VFF22_20195"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            25780..27918
                     /locus_tag="VFF22_20200"
     CDS             25780..27918
                     /locus_tag="VFF22_20200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016703414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX19176.1"
                     /translation="MLDTLSIRLKIILLSGLCLLGVIGLIVSLNLYQSSQNNQLIRTS
                     SSKMLTESGENLLQAKAAEQASKLQSTFGDSLLILTVLSDQVANLRNLAAQRGLAPGA
                     LREELTQSLKTTFDHNPQLLGLWLLYEPNALDGQDSEFLNDAARSSNDKGRFTSGWNR
                     GAGQSVNLTISENVINNTELSISGVPYNSWYTCPRDNQRTCLMAPYVETLGGTAQLMT
                     SLSMPLIVDDKVIGVIGVDLALGALQAAVSDAQQTLFNGAGSLLIVSDSGVLAAYSKD
                     AGQIGKHIDTALGEASKPVLSGLQLNKPVVLTQDELIRAVYPVAPILGAAVWGVVIDL
                     PKQVLLADSVELQTLLDQTQTRDTLKSLLVAVCAGLLGLLIIWLTASGITRPINSVAQ
                     MLKAIASGDGDLTQRLNYSKKDELGELVNWFNRFLDKLQPTIAQIKQSITDARGTADQ
                     SSEIARQTSEGMQVQFREIDQLATASNEMSATAHEVAHSASNAAQAARGADEASRDGI
                     TLIERSTRDINALASEVSKAVAEVEALAVNSEQIGSVLEVIRSIAEQTNLLALNAAIE
                     AARAGESGRGFAVVADEVRNLAKRTQDSVEQIRLVIERIQTGTRGVVATMHSSQTLAK
                     NNAEQIQQAVQALGKISDAVTVISDMNLQIASAAEEQSSVAEEVNRNVSNIRGVTEAL
                     TGQAAEAAKVSSQLNARANQQMVLMDQFRV"
     gene            complement(28379..29290)
                     /locus_tag="VFF22_20205"
     CDS             complement(28379..29290)
                     /locus_tag="VFF22_20205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HZX19177.1"
                     /translation="MIEMDQVVPWKGLIALIDPHYPKGEGGRPAYPLMAMLRVHLMQN
                     WFGYSDPAMDEALYETTILRQFSGLSLERVPDETTILNFRRLLEKHELATGILAVING
                     YLGDRGLSLRQGTIVDATLINAPSSTKNKDGKRDPEMHQTKKGNQYYFGMKAHIGVDD
                     ESGLVHSVVGTAANVADVTQVDKLLHGEENMVGADAGYTGVEKRPEHDGREVIWQIAA
                     RRSTYKKLSKRSALYKAKRKIEKAKAQVRAKVEHPFRVIKRQFGYTKVRFRGLVKNTA
                     QLVTLFALSNLWMAPQHLLTNAGEVRL"
     gene            complement(29454..29900)
                     /locus_tag="VFF22_20210"
     CDS             complement(29454..29900)
                     /locus_tag="VFF22_20210"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZX19178.1"
                     /translation="MPAIDLNDGFLQLHARLDRLESESAIRRLFSRYMQLCDVPCVQP
                     EGVAESLGGLFSPDAVWQEVGRLYTSKFGVFRGRTAIINMLMSYLPPSPHLRTYGHLL
                     GSEHIDVEGDQARGSWLMQQVSDYEEGSADWSDQVCRQAGSTVCVF"
     gene            complement(30181..30458)
                     /locus_tag="VFF22_20215"
                     /pseudo
     CDS             complement(30181..30458)
                     /locus_tag="VFF22_20215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007243727.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
     gene            complement(30478..31722)
                     /locus_tag="VFF22_20220"
     CDS             complement(30478..31722)
                     /locus_tag="VFF22_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX19179.1"
                     /translation="MSVEHSIANAATPRIRQATGRREGVVLMLGSSLTIMGAVMVAPI
                     LPKLAAEFGPTEPHADLLVPLAVTGPALAIALCAPLAGWLADRVGRKALLVAATLLYA
                     LLGIVPALLNDLTAIVGIRLLFGCAEAAIMTCCAALIADYWQGEERMRYINLQVVTIG
                     LVGALFFVVGGVLGEQSWRLPFYLYLLPLLLLPAMIKVLWEPVRARVETAVNEDDEQV
                     LISTLVSGYLLILGGMILSLIVPVQAPILLVELGISSSTMIGLSAGLGLLATLGGSLI
                     WPLLRRGVGIAGCNVVLLALLALGLWLLTRAQSYNEILLAVSVHGLGAGLLVPNAMAP
                     VMNALSANTRGRGLGVFTACLYIGQFVSPLAVALVGAFTNDLRASIHWLAIASMVAAS
                     LWLVVSLVQRKRAVGQPMSSHS"
     gene            complement(32160..32807)
                     /locus_tag="VFF22_20225"
     CDS             complement(32160..32807)
                     /locus_tag="VFF22_20225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009621662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZX19180.1"
                     /translation="MQSAELGRRCLDTFTQLIPASGSVFYRIAAQLEPHDFLLQQMRS
                     DMHERYLSYYRRLDPLQPSTCQLTGCSVVPLRLGMRAQNDTANQRYGDFLQGFGVVDV
                     VEVLAFEQGLPIAGISLLRGRDLGLFGAHELVQLQALQGMFEIAVKQLSPSAPAQQLT
                     PREWEVAELLRAGLGNKAIARQLGVGLPTVKTHLINLFRKVAVSNRTELVATLFL"
     gene            complement(32869..33228)
                     /locus_tag="VFF22_20230"
     CDS             complement(32869..33228)
                     /locus_tag="VFF22_20230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012724441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZX19181.1"
                     /translation="MTPIKQGTTFTELDAWGSVADLGSEILEGEGKAFGRMTFGAPTD
                     AISAGYFAVSKSKFRMVYPFNEQAVVVFGEVIMTDESTGQSTRYTAGDSWFVTKGTAV
                     LWDVLSDSFTKHYLAVV"
     gene            complement(33272..34570)
                     /locus_tag="VFF22_20235"
     CDS             complement(33272..34570)
                     /locus_tag="VFF22_20235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZX19182.1"
                     /translation="MINIETPTYYTATKKYDLSFPSLEADIEADVVVIGGGFSGINTA
                     LELAEQGITNIVVLEGRYLGFGGTGRNGGQIMAGIGHDLEKIRKDVGEEGVRAIFEIS
                     DQGAEIIKSRIERYNIDADFCHGYGYMGFNARQEKTLAAWEKEFKALSPQHEIRLLVG
                     SELKQMIGSDAYSSGLLHMGAGHVHSLNLLLGEAKALASHGVKIFEYSPALEVSYGER
                     VTVRTAKGSVKASKLLWACDSFLNKMEPELHKSTINTYAFQIMTEPLSDELVERISPI
                     RGAYSDIRPVIDYYRVTRENRLLFGAATPFVEHIPRDLKAWNRNLMLKIFPYLKDVKI
                     DLAWGGPMACSPNLFPQLGSLPQHPNVFFVQGYSGFGVTPSHIICKVLAEGMSEGSAR
                     YDLISSVRRIPVLGKDQIRPLLLTAGKSWHQLSGYWSGRR"
     gene            35173..36246
                     /locus_tag="VFF22_20240"
     CDS             35173..36246
                     /locus_tag="VFF22_20240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017846950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX19183.1"
                     /translation="MTPQHEHCECRTTGWSMTLIRKHRTDSVERIVMSTYDVVDSTFQ
                     APSTALSSYSRHVSSNDIDEHAAHVSGWHVNYDQMSAGLFQGELSEFKLDWMQVMRDR
                     TNQSLLKKGSAKSGTITFSVPVSYAGNCYCGGHPMQDGNALVAHADDLPQLRTPDQMD
                     LIVVAVEQEILERELELHGITFDSAHLPKVYQLQGLALPNNLRMILDDLERGVALPGL
                     PDEVWRHQVRDAIMGQILDLLPSVQPIELTPSARKRTVDRACEYALAHLDEPLSILDM
                     CRTLGASRRKLQYCFQETLGTNPVAYLRALRLNAARRELLKGASRVSVQDVAARWGFW
                     HLSRFSSEYREMFGERPSQTLHR"
     gene            36726..38357
                     /locus_tag="VFF22_20245"
     CDS             36726..38357
                     /locus_tag="VFF22_20245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HZX19184.1"
                     /translation="MDKKFALYPLAMSIMASMSPAVQAFQIDSADPDLRLRWDNTLKY
                     STASRLKGASDQLTAQPNHDDGDRNFSRGVISNRFDLLSEFDATYKNIGARVSGAAWY
                     DSVYNEGNDNNSPATANAFSVDNDEFTRATEKLHGRKAELLDAFLFARFNLGDTKGTV
                     RAGRHALVWGESLFFGANGIAGTMAPVDAVKAASVPNTQFKELIRPTEQFSGQLQLTP
                     DISIGAFYQWRWDRTRLPASGSYFSVLDTISDGAERLIAGPNPVIPGGEPLAFFREQD
                     MKAKDSGQGGVQLRFSAGETDFGLYAVRYHDKTPQIYIRPDAGVPDFASGRLGTYQWV
                     YPENIRAFGASASHTFGDYNLAAEVSTRRNMPLSSDGVTIVDGTGDNDGDARYAVGNT
                     LHAQVSWLATLGPSFIANEADFVGEVAWNRVLSVTKNRQALNPLADRDAANVRFVYEP
                     RYRQVFSGIDLSVPVGLGFGMGNSAVVGAFNGDKVGDVSIGLTALYLNEWRAGLTYTH
                     YFGPAGTSVDEDNHISFKQSLKDRDFIALSIQRTF"
     gene            38373..39725
                     /locus_tag="VFF22_20250"
     CDS             38373..39725
                     /locus_tag="VFF22_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003200366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HZX19185.1"
                     /translation="MTRFFLPVLSLLLLCAQQSLAAVTAEEATKLKGELTPLGAERAG
                     NADGSIPAWTGGMTTPIPGDKPGGRRSDPFQNEKPLFTINASNAAQYADKLTDGTRAM
                     LAKYPDSYRLDVYQTHRTAAAPQWVYDNTLTNASRARIDNDVVVGAFGGIPFPIPTSG
                     AEVMWNHLLRWRAPAARFYATAYQLTADGRAVLASEASSVQQVPYYFKDTTLEEYAKS
                     NLYFNIRVNLTAPAIRAGEGVVGRQNLDAAKDQAWVYLVGQRRVRKLPNTCCDTPSPT
                     MAGVMSFDELEVFSGRLDRFDWKLLGKQEKYIPYNANKLLQPTSDKDVLSAHHLNPEH
                     VRWELHRVWVVEATLRDGQRHQAARSLYYCDEDTWTCVLGDRWDANGQLWKTTWGQVI
                     VMPDLPGNFMFAFGFNDLLSGAAMVAGLFNEKPVHYELLERLPDVEFTPDALANDGIR
                     "
BASE COUNT         9135 a        11351 c        10676 g         8687 t
ORIGIN      
        1 gcgttcggga tagtgcaaac gagcaaggcc gctgctcgaa accgctgagc atgtccgccg
       61 gcagctcttg caacgataac gacgtaagcc acgcgaccag ccccagggag caaggtgctc
      121 agcttttgct tggcaatgtg ggcagctctg taacgctggc aagctatcaa gaacctccgt
      181 tgtaggtggt tcctatagcc cctgatgcag tgcggatttc tgcttaatgc tgcgttggct
      241 cacctgagcc aaccagcgct gaaaggcttg agtatccatg gtgggcagac ctcccgagcg
      301 gatgcctgcc ttaagcttag tccttgcaac acggtcttgg gacagagcct ttttaatgag
      361 aaactttcag gcaagcacac tctttgcaat acggccatcg cctctacgca tcaatgaaat
      421 agctctggca agatggcgta tcaactcgtg gctcaccggg gaaagcttgc gggatgggcg
      481 aacgatgatt cgcatttcgg tactgttcat tgcctcacat gccagcggga tcgccttgaa
      541 cagccctgat gtcagctcgg aaaccaaggg cagttctggc agcaaggtac tggctatgcc
      601 tgcacggaca aggtccagga tgacctgcag tgagttggcg gtaaccaccg gctcaatcgt
      661 aaacgcctca tccttgcaag cttgagtaac cagctcccta acgcggtatt tggggccagg
      721 caaaaccaaa ggtgtattgg caagatcggt taacgtcagc acatgctttt ttgccaacgg
      781 actgtttttg gacacaatcg cgcggagcgg ctgctcgaac gcatacaaac tgcggagggt
      841 caccgtgctt gtgggcgcaa aagccaggcc aatgtgcgct tcgtcatctt ggatcatccg
      901 ctgtacttca aaagacggag cgatgatgat ttcggtatga gcatcggggt attgcttgaa
      961 aaagttttgc agctccacga tcgcttggga gcccagcaat ccttctccga cggcaatgat
     1021 agtcgtgcct agaaaacgcc ctcgcaactc gctgagctga ttcagcaacg cttcatgctg
     1081 gcgagccctg gcacggtaat aatcgatcgc aacctgccca gcctcagtca agcgaatccg
     1141 atgagtgccc tgctctacca gatcgatgtc cagttccgcc tctaacttgg caatctgtcg
     1201 actgatcgac gagggggcaa tgcctagctc gtcagccgct gcacgcatcg atcccagttg
     1261 atggctgagg aagaggtatt tgagttgttg gtcatggaag tccagtagca cattgggtct
     1321 cattacggtc accgctcatc aggctgcttt ctgtccgact aagacgcatt ttgaatcctc
     1381 aacgcgcgtc ttcacggggc cgtccggacg ccttcacccc tggtaatgcg gcccgcttaa
     1441 gccaagtgtg cccttttcac agcaccaccc gatgcctgtc gcatcaggaa caattcttca
     1501 cggggctact attactcgcg attactcgct cgcgagagga cagtcatctg cttcatcaga
     1561 aaactcagcg ggaaaagtcc ggtttttccc aaaaattagg aagtcttctc agcgccgaag
     1621 atcaggcgct ctggcgagtc gagtggttca aacaagccta ggatgctggc cggatggcgt
     1681 cttgaatttt cacgacatgt gtaaaaaaat cagcaccata aattggcagt cacgctgcca
     1741 aagaagcgct gggcggtagc aggaggcgac atgatgttgt tcgccctgtc gagcctgggg
     1801 atggcgcagg tggcgacgcc tgtaatgtgt ggcatggccg ctgcggggtg agtcagccgt
     1861 cgtttcgact caccggctct tcaccattga caggcagaaa agctagccag cgctagcctt
     1921 ctgccgtaac aggcttacac gtatggaatc acaggttcaa cgcccacaaa gatagaacct
     1981 gcagcatcaa aggtgccaag gctcaaggcc gcatgctgag tgatcaccat cgaatcccgg
     2041 gcgatcgcct gcaaacggtg ggacttttga atgaccccga tcccagcgat ccggtaggct
     2101 tcctgaacga tatcggtact ggtatgcgca gcgtgcgcag cactcaaaag aacaaggctg
     2161 tgcagttcgc gcgtcagttc gcccttgctg atgagcatgt cccaagcttg ctcagcggtt
     2221 tcgtaaaaaa atgcccgcgc acttcgcaac tttgcctcac attgagcaat gctggtgcgg
     2281 taatacgctc gatccgccag gcgtgatgcc cccggaatga tcttcgcacc gccgcccatc
     2341 tcgatcacgg tatcaagggc tgcacgggcg agacctatgt tcacgacagc gtgaacctgg
     2401 gcctgataag ccagtgctgg atagcggtac aagggctcat caatcgggct aggcaactcc
     2461 cgctggcagg tccactcgtg agccacgaac gtgttttcca ggcgaaggtc gtgactgccg
     2521 gtaccctgca tacccacaac gtcccagctt tccacgatct cgacttcagt ggccggcatc
     2581 acggccatgc gcacgcttgg cggcgactta ccgtcaggtg agtccacctt aatgcctacg
     2641 cccatccaat ctgcgccttt acagccgctt gcgaatttcc aacggccgtt caggatccag
     2701 ccaccctcta cggcttctcc ataatgggga ggaaacagac cacctgcgaa gacttgatca
     2761 gggcctgttg cgtacaacag tttctgcgtt tcatatggca acctggccaa gtaggtattc
     2821 gccgaaccga aggctgagac ccagccggct gagccatcga cttccgagat ccgctcaaca
     2881 cgacgcaaaa gctcagcagg tggtagcggc tcaccgccaa agcatgcggg ggtgctcgcc
     2941 cggaatatgc cggctttttt catcaggttg atcacatcga ccgggacatg tttgccctgg
     3001 tcaaattcca gacggcgcaa tcgaacttgt tcgatgagta cgtccatggc ggcttgccag
     3061 cccccctcac ccagctgttt tacagataca gtacattccg gcaacgtcag cgtgctcatc
     3121 ggtttttcct ttaaagattt tgaaatagtg ggcgctagag cgaagacggt cagcctgctg
     3181 aagcagtgtt caacgatggc gccggcgaga ggccaagccg atgaaattta cggccaaaat
     3241 aggtcaggca ttcgccgtct tcacgcacgt tgatatgatc gatttcaacg aacagcaccg
     3301 aatgagtacc cacctctttg acgtcgacga ctcgcccttc aaggctcgcg agcgcatcac
     3361 gcagtacggg caaaccgctt ttcccctcaa cccaggcatc aggattaaaa cgctcctcat
     3421 gcggtacata ctccatcccc gcaaaagtac gcgcgaggga ctcgtgatcg ccgtggagaa
     3481 tattgatgca cagggttttg ttgcgcagtg ctgcgtcatg caccgctcct ttctggttca
     3541 gacatacaag gagggttgcc ggcgtatcag taacggaaca gatagcgctg gctgttacgc
     3601 caaaacgacc atcaggcccg tcagtgctga cgatatttac ggcagctgcc agcagtgaca
     3661 tggcttgtcg gaaggtgttt gacgcgtttg ttgtaattgt tgtcatggga gtaacctttg
     3721 aactgattca gggggaatag acattcacaa cctaatgaac caaagcgcgt cgcaactcgc
     3781 ggcggagaat caaaaagtca taggatgtaa ccgataccgg caagggtatc ggtcgagttg
     3841 atcagcttga tcagcttgcg ctggatcgtg aaactgtcgc cgtcacgcag cagctcaaag
     3901 gtgatgtcgg cggtgtaatg cttgagcagg tccttgcgaa actcgcgcag gttctgcgcg
     3961 cagcgcacgg tgaccacgcc gtcggcatcg cttagcaggc gaaaacgcga cagggtccgt
     4021 acggtacggg cgcgcggcga ggttgagatc gactcgccgc tggtcaggcg cttcacccgc
     4081 aactcacgca tgtggtggtt gtcataggcg tagttgaggg tgttttccag atcggtctcg
     4141 tccggatcga ttggaatgat gtaggtccct ttaggcgccc acagagccag ccacgcctcg
     4201 aattcgctgt gatcgagcat gtccgcttcc tgccagatga aagcggtaac ttggttgagt
     4261 aattgcagat tgatcatggt gttctccgtg ccttagaccg acatcatctt tttccactgc
     4321 tgataggcag cgcgcattcc ggtctcagcg ctcacatcgc tgaccaggcc gtcgtcggac
     4381 ttcgtctcac cggccaggcc gcgattgagc atgatccaca aatctttacc ggcgcttgag
     4441 cccttctgta cccgctccca cgcttcggag tcgtccggcg taccaaagcc catcggaccc
     4501 tggaagtgtt cgtgaagacg caggcggtag cggttggccg tggcggggcc gccatccatg
     4561 gtgatcaccg agtggtgaat ctcggtctcg tcgaccgcga ttggttgcag cacgcggaag
     4621 aacgccatcg agcaagcgac gttcgggaac aggttgaggt tgaagcccga accgcccact
     4681 gcgcgggtga tacggcgcac gctcagctca tcgtggcctt cgtcacgcag ctcctgagcc
     4741 agttcctcga agcgctccgg gatcggccgc tccatcagct cttcttccag atccaccagt
     4801 tcgggaatca tcaccatgac gctgtggcca ttacccaggt cttcgacgta gcccgggcct
     4861 tcgacgaaat cgaacagctc ttcggtctgc ttatccaccg aggacagaaa ggacttgtgc
     4921 accaacggga agtggtacgc gtcggtggtg ttttccagtt gaatcttcca gttgcctggg
     4981 aatcggaaac gatgctcgcc ggccaccttg atcgggtaac ccgcgccctg cttcataaac
     5041 aggtcgatcc acttcttggc tgggccaagg aagtccacca acggttcgat atcatccttg
     5101 aacgtggcga aaatcatgcc gttgtatgct tcgacgcgca ggctcaccag cgggtgctca
     5161 gccttgtcga gcagatcggc gtagctctgt ggcgacggta tcccgcgcag cgaaccatcc
     5221 aaggcgtagc tccagccgtg atacggacaa acgaagctgt tggtcttgcc ttttttacct
     5281 tcgcacacgg tggccgcgcg gtggcggcaa cgattaagca gcacgtgaac ggctttcttg
     5341 cggtcacgca caacgatcac cggctgcttg ccgacgtaag tggtcttgta gctgccggcg
     5401 tccggaattt cgctggcgtg ggctacccac acccaagtgc tgtagaaaac cttgtccaac
     5461 tcgacgtcga acagcttggc gtcggtatag agcgaagtgt ggacacggtc tggctggacc
     5521 agctccgaag ggctgtcgac aagattgacg gcgtttatta gctcactcac agttaatacc
     5581 tattttttta tcatggtgtg acttgactaa taacgaaact aaccttgcgg gcactgccta
     5641 tcaatcgccg ctcctgagaa gttcgttgcc tccaaagcaa caagaatcaa ccctcctctt
     5701 agcccgcgtt atttgtcctg tgttgcagct cgatcatgtc ggcgataggg cgttacgctt
     5761 cacccagcaa cgcacagagg ctcggagccg taacgggttg aggcgctagg ctgaccagta
     5821 agtgaactgc tgataaaagc cgtgactgaa aacatgccaa acggctgaca gacatgaacc
     5881 caaagatatg gagtcggtga tttctgtggg aggtggaata ggtcgtcagc agacttccgg
     5941 caaaaaagca ggctctagct tggacgtatg acagttgaac agtgaggtgg gctgttgacg
     6001 gtgcggggat gagatcgcag agaaagcaca tgccaggctg caatggctcg gacagggggt
     6061 tggatcatta cgccacggga aaccgggcag acttctgcgt ggtcgttgaa cacataagcg
     6121 aagatgaggc tgggtctgaa agggtgcagg tcagcgcgta agagcatggg tttcccctat
     6181 ccgcgacctg agtccgtata gcgaaaactg accagctcag agcggatggt gaagctccat
     6241 ctgtacccag aaggaatcaa ggcagataga tcgactcttg atcgggctta caacggcttg
     6301 agccgcctgc ggtgaaagtg acgtcattat tacgcttact gggtgctcta caacaccgcg
     6361 cccaggtggg cttctaatgg ttactgcatg gtgtctaatt acctgggtgt aaaagcggcc
     6421 ctgctgcaaa cgttaatctc ttgcgcaggg tcgcagtgtc gcagtgtcgc agtgtcgcag
     6481 tgtcgcagtg tcgtgcgctg tcaggcttag aaggacgtgg caaataccaa ctgggtgtac
     6541 aggttcagat cgtcgttacc gagttgagta ccgccttggg cggcgctctt ttccggttgg
     6601 taaaggccga tcagcgggat gatcataagg ttctcggtca cgccccactc agcgagaata
     6661 tcgatctcct ggccgctggg attacccagg ctgcgatcga tggtgtcgaa gtcaaaatac
     6721 agcgcgctga tagtcaggtt ttccagcggt gacaccttca aactgaggtg ctggacgcgt
     6781 gtattagtgc tgaagggacc tgcatagcta cctgctacct caccctggaa ccaagtaccg
     6841 aaagcgcggc tgaagccata aaacaggggg tcgtaacctt ccgaaaatcg gctatagcgg
     6901 taactgacgc tgggcgacca ggctacgtcg gcaaaggtcc agccagcttc cagataccag
     6961 gcatcttcgg tggcggtatg cggcttgtcc tgtttggcgt attcgccgga aaggaacagg
     7021 ttttccacgc cggcgttgcc agcggcgcgc aggctgtagg ttttcatgcc gtcgcgctca
     7081 agttgcgtcg gtgaagcgta tttttcgtcc acatcgattg tgttgatgta agtcaggccg
     7141 acggtgcccg gctcagcaac gtgctccaag gtggcgacat acatttcagt tttggcttgt
     7201 ccgcggttat cggacttcaa ccacatcagg tcgctgcgcc aaccctcctg accaccgaga
     7261 cgcagcacgg ccgttttgtc gaagttctta cgtgctccca agtaggtgct gccaccacgg
     7321 ttaaactcgc catcggccag acccttaccg aggtttgtgc catcgccata aattagaaaa
     7381 ccatcaccga ccgctatatt ctggcgaccg aaggacacgt ctacgccgtc tttacccagt
     7441 gcctcgaaca gttcgccaga gcgccagcca gcgtaggcat cttcaatctt ggtggtgcgt
     7501 tctgaaccat tactaaagcc agtggcgtca ccgtcacccc aggttgcgct gctcagcaac
     7561 gagaacgcgc catacgcagt accagcgcca gccagaccct gatcaccact gattccgtac
     7621 ttgatataac cttcgcgcca ggtagacgag ccctcagact tactaccgga gttcgagtaa
     7681 ttctgctggc tgttaaagaa accgaacgtg gcttcgaggt tagcgttgag gtgagtatct
     7741 tcatctgcgt acagttcata ggcgctggct tgtgtgctgc cagcaatcaa cgccgcaagg
     7801 atggaaatac gaaagaagga cgacttatgc atgtgttggc ttccattatt atatttaaag
     7861 gtaaggaact tgattaggcc gggactactt cacctgcgtc actgacatgc aagcgacaag
     7921 ctgaattatt aatacagcca ttaccgaact tagcaagtgg gtttagcaag attttaacga
     7981 gcagttactt caggtaaccg ccccgacaga aacagtctaa ttattattat gaaaaatcaa
     8041 agaaacgacg ggcattgcaa cagcaattaa tttaccgagg gctacgctca gtgattgagc
     8101 atggcggata ggcgcttacc aacaagcgcc tacctagcct ccagccttct tagctcatat
     8161 caggctcgaa aatgccccgc ttcatataaa acttcaccca taaaacgcta caaatggtaa
     8221 tgaacaaaag catgccacca gtaatggcat aaacagagct ggtcaattcc ggcgaggggg
     8281 aattattgat ggccacatag acgattgcga caattgcaaa gatttgcggc acagggaaga
     8341 acggagagcg atatggacgt gggtgatccg gcatccgctt acgcaagata accacattca
     8401 ggtgcgcaat caggtaagcg ataaggtaac tggtgctggc cgcgataaca aagaggatga
     8461 tcgagtccat atccagcgac aagtaaggaa taacgatcga gatactaagc actgcagtgg
     8521 ctacccatgg cacattaaaa cgattaactt ttttgaaaat gggtagtagc tgcccttgtt
     8581 cagccatccc gtgcatcatt cttggaacag ctgccagcac agtattaata gaacttgagg
     8641 tcgcagcaag tgcaacgacg gctgtaatca ataagccggt agagctacca aatacagcca
     8701 tgataaagtc aacatagggc atccctgcac taaccaaggt ttccgagtcc atataaaagg
     8761 ttatgccata gccaaaccca acgaacagga agaagattac gaacaaggaa agatgcatgg
     8821 cgcgaggtac atttttatgt gggtctttta cctcgctgat taacgagcaa atatactcta
     8881 tgccgacaaa acaccacatt gacagcgcaa tgaaaccgat gaagccgaag tcagttacgc
     8941 tagaaaaacc ccagtcgacg ggcgtacccg ttaactctgg atgtggttgt gctactcccg
     9001 tgatagccac acatgacacc agtgcaatgg tactcaccat gacgagaaca agcaggtttt
     9061 gtattcgcgc aaatacgtct gtccccatta agttagtaag ggtaagtccg accaagataa
     9121 atagagatat tgccttatca ggcacgaagc cgggcagcag ctgggctaaa ataatatcaa
     9181 tcatgaacat ttcaaccggt accgccagca cggacacaat aatgtaaccg gcaaaaacgg
     9241 caacaatagc tgggacatga cccattgctt tttgggtgta ggtagccagt gttccttttt
     9301 gaggaaacat cagagaaagc tcggcaaaac tcatcgcatt acattgtgaa atcgccaacg
     9361 ccgccaccat ggcagccaga aatgtcatgg caccaaaacc aacgccctgt gccgtcgata
     9421 tcatcgcgcc ttgagccaca accaagccga tacaaactgc catcattgtg aataatccga
     9481 actttttgga cagttgcgga ttcgggctag tagccgtgag attggaacta gatatattcg
     9541 tagactcgat catgtttagt gtccatttat ttttatttaa gggctaatac tcgattactt
     9601 cagaggcgat caaaccggta tggagcgggg tcaacgagtg gggtcatatt ggctacgagg
     9661 tctgctgcta gttgcccagc agcgggcccg gtaccaaaac catgacctga gaagccagtg
     9721 gcaatcgtca gcccaggaat ggactgaata cggtcaatga cagggtttga atctggggtt
     9781 atttcaatct gccccgccca agcttcggaa atacgtgcgt tttcaaaaac cggccaggca
     9841 ttgcgaaggt tgcttaaggc ttcctgaata aggccttcat tgacagacgg gtctagtgtt
     9901 cgaacccgct caaacggtga ggtttggtct gcacgccagc ggcgcttgag ggcaaggtct
     9961 tggaggaagg gcttgcctaa agaaacgcgt aaaaaggaac cttgcccacg aagcagacca
    10021 aagtaccggt ggccaattaa taagtgatcc aaggttagct tagcgtctaa ggccgcgcgc
    10081 tgggtgatga tgtaaccacc atctatgtgt tttcgaaatg aaaagtcagg gccaccaaca
    10141 gcaatatcag ttggaccatc cattggggtg gtccgcagca cggagcatat aagaggcagc
    10201 gttgggtaac taacgccaac attacctaga aagcggcgag accaagcgcc accggctaag
    10261 agcacctggt cacagcgaat ctcaccgtgt tcggtaacga caccgctaac cttcccagcc
    10321 gaagttgaga gtgttctaac ggcacagttc tgaattaata tcgccccttt ttcgatagcc
    10381 gcttgcgcaa gcgcagaggt ggccagatgg ggctctgctt tgccgtctga aggggtgtag
    10441 agagcacctg cccactcccc tttgcctcga ggtacacgct cattaacctc gcgtgcacta
    10501 agtatttggg agtctaagga taaagactcc acggacttca gccagctttc atgtaacgct
    10561 aagcgttctt gattttctgc aagaaacata ataccggatt gctgataacc aacctcgcgg
    10621 ccaatacgct caggtatacc tgcccatagg cgatctgccg ccagcgctaa tggcacatca
    10681 tcagcgtgcc gattagtctt gcgcacccag ccaagatttc gtgaggattg ttcgcctgca
    10741 atatgccctt tttccagcag gacaactgga atattccgct cggctaaggt caatgccgcc
    10801 gtcacaccaa taatgccacc tccgataatg acgacagtgg tggattttgg taattgatca
    10861 gtagtcctca ctgactcgag agttttcgtc atgacaattt cctgaggtgc ctgcgagagt
    10921 acttgaaatg ttcgacagcc gttactctag cgttatgtta actttattta gatttgatgc
    10981 accccggtac gctgtaacct ccacctctac tttgtataag gttgagccta atggtgggca
    11041 agtgatcgta gtggccggat tgatgccttt gaatttctgc gcgatgaatc ccatcacagc
    11101 atttacgttc tcgggtgatt gaacaaaaac acgagacatc acgacgtcag ccagagaagc
    11161 gtccaatgaa gccaaggtct tttcaataat ccgaaacacc tgctcagcct gctccacggc
    11221 atcctcagga atttccttgg tgtctggatt acgccccgca gtgagggata cataaatcca
    11281 gctgtctacc gccaccgctc gagagtagct gcccaacact tcaaatttcg aatcggtgtt
    11341 cacttttaca atattggtca tgatgagatg ccttaaatat taaaaataat caaatagcga
    11401 ctaacgaagt actggctcat cccatagatt aagtttgacc ccaatgcctt tttcaagcgc
    11461 attgcggtaa accactgtcg cccaggccac gtcctcaacc ggcataccgc caacagacag
    11521 aatgatgatt tcttcatcgt tttggcgcgc cggcgttagg cccgcacaga tgtcacctag
    11581 atcctccagc gcattgcggg aaagttttcc ttccccgacc atatccataa agcgcatacc
    11641 aactaacggg attaggttat gggccggctt aggtagctct tcataccagg catcgtaaag
    11701 tcctgtgtta tcaagtactt tgcgtacatc tgcttgttcc atgccgtcgt ccaaacggca
    11761 aaaggcaggc atcgcaagga aagcacccgg cttcacccac tcgcgcttta ctaagggata
    11821 cgtcgatgga tcgccgacat caccggagtt acagtaagta atgatgtcag caccgcggac
    11881 tacgtcttca actgtatcga cgacattgac atttttgatt tgaggaaata cttcgttaac
    11941 ccacgtaacg aacctattta aatttttttc tccgcggcct ttgatgttga tagtgtcaat
    12001 ttcagggcac gtcgacataa atgctgtaac agatgttttg cccatgacgc ctgggcctaa
    12061 caagccaata atttttgaat ttttccgcgc caggtgacga gcgcctacgc caggaactgc
    12121 cccggttcga taagcggaca acagattggc tgacatataa gccagtggag caccggtatc
    12181 gatatcattt aaaatgaacg tcaaaattga acgcggtaat cctttttcgc gattcgcagc
    12241 gttagagccg taccatttca caccacaggt ttggaagtcg ccacccaggt aggcgggcat
    12301 agccattaag cgacgatcag gtgtaggctt cggcatgttg ggaaagggag aatctgcggg
    12361 aaatgtaatc tgtgagccat gagagtcatt attcgcaccg gccatgcgat aatcaccttg
    12421 ctgaagcaga ataaacatct gctccatggt gttcacgcat tcggccatgt ccatcactcc
    12481 ggctttgatc atatcctgct cggagagata aataaaatcg atcttagtat tatgctttga
    12541 catagttttt atccgcaata ttcttgacac atttaaatgc cgtcattggc attaatcgaa
    12601 aagctgttcg cactttcttt tcaggcgcct agctaaggaa tcatgtgttt tttgctaaac
    12661 acagctctac atcacagacg ttaatcatcc acacgcgtaa ataaatatcg gcgcgccatc
    12721 ggcatacttg aaaacgcaaa ccgtgttcca gtttctcgac gtattggcca gcccgagcaa
    12781 gaatctattt atataagcct gacagccgcc ttcgcacctg gcgaccactc ggccactcaa
    12841 ataaataaag cctttcacat ctctactcga cccctctaga tcaagaaaaa atgcttatgg
    12901 acctgcttta attttccggc gtaggacgat aaaaaagtta cccctagcga gttaaaagtc
    12961 tgagacgatc ctagagtcat tacttgccgg gatattgaat ttttgcgaca gaaaatcgat
    13021 tcgcatccgt tttgccaaga aaattactgg gcggcggcaa aaactaaagg cacacctgac
    13081 cttccggtac ggtgctgcct ctatgtctta ttccgaacgc agcgtgaaga gcgcgccacc
    13141 attcagatcg gtcatgccca aggcttcagc ctgcgtagga ttgcctgcct gttcacccgc
    13201 ccccttcgac cgccagccgg gagctgctca gcatgaacat ttccagcctc aggggtgcct
    13261 acggctgtcg cgggacaatc cataacgcga tctatgcctt gccggtcggc gagctgcgta
    13321 agtaactgat catccatctg tctgcggtaa agcaagacaa tacgcaggca gctctgatgc
    13381 gtggatccgc gtagccagat tcccaagatg gtcagcatca tgtacgccgg agattgaaga
    13441 ccgcctgatg ctggggccat cgagaccgcc cagaaaaccg gcctcgcgat ttactgctgc
    13501 gacccgcagc gcctggtgta aagttaattt aagaaatcag tcacgcttca cccctataac
    13561 attacattca agatcatcaa aaaaacgatg cacggtatcg gcgacttctt tagactttaa
    13621 ttccattaaa ggtataccgc ccccctttat ttctgcatgg gacacgaaac aggaattgca
    13681 ataagcatct actacgacct gagtccgaga gtatgtgacg ggatcatctg tagctgccag
    13741 aactaacact ggcgccgtga caaggccaac tcgatcctcc atatcatatc ttgcaactgc
    13801 cagatgcccc tctaaaggcg ttaccccagg agcaagcgca tcccgaataa atcgatttaa
    13861 cacatcagtg tgttctggcg ggtataactc taaacgtttt cgccaaagct ccatcaggtg
    13921 tgagccatcc tctctcgtct cggctacatc tacgtccagc gccgccatgg cttctcgaaa
    13981 cgcggcatcg acgatatcac cagcagaaag aaccacagcg gtgacacgcc ctggagcgat
    14041 agccgctacc tctgaagcca caaacatgcc tgtgtgatgt ccgagaatag aaaatttctc
    14101 tatatttaat gcatcaacca gtgccaaaac accttgggca tattgctcaa ccgtctgtgg
    14161 agcagcaaat tttgcagact gcccaaaacc gtacatatcc atagcgatgg tacgccaacg
    14221 agaggctaat agcggttgga tttccgcgaa ttcatccagt gagcgcggtg tttgatgcaa
    14281 taaaatcaga acgtcacctg cacccgactc cgcgtaatga agttgaccca ggctcgtatc
    14341 aatataatta aatatgcgct tatcagtgac gccacggaca gaacaagagc ttaaaatagt
    14401 catatcgtta accgtttttt atagtgagac atttaaatcg cgtcggattt tccaagaaaa
    14461 gcttcgccag ctatgcagcg taacaaccat gctacttctg aaagaccgct gtaatcgacg
    14521 cgctactctg accctgaaca agatgcactg aactggtgca tgaacaccca aaatggatcc
    14581 tcgacccgag cctcgacttc ttgaacttga gtatggcttt gttacagaac gccccacttg
    14641 tagagcctct ttttagtgct gcgatgcgcc accaccggcc agataacctg cccgccatcc
    14701 tgttctggac gcttttccaa accagcaggc ctgacggccg cctccacccc ttacaacaat
    14761 gcacctcgca tcaaagcaca acccctctag cgcggcactt tatcgagccc gatcaagaag
    14821 aactctttaa caaatctgtt tgcgcttgcc ggcgtactcg gcatcgacga agataacctg
    14881 cttcaccgga aggctcagca ttcagaaccc tgatttcttg ccaaaatcag gaagagtttc
    14941 tagagtcctc ttagtggcta ggctcaggcg ctgtagcgat tttttcgttt aaagaaaacc
    15001 tagaattctt attaagtatt gaatttttgc gacattctca aactttataa aaaaaagacg
    15061 gcacgatacc tgcaaggact caggtgacaa ctttataagc aggtgcagat tatgtcagta
    15121 ccctcattgg cttcgcccta tttaagcatg cactcccacc aaccatggtt tgtgctgcaa
    15181 gccgctaaag aattttcagt ttcgttatcg gacaatccgt taatttcaca cttttatagt
    15241 tttgaaacaa aatcatcctc gccattcatg ctgtcggttc cggacggctg tatcgatata
    15301 atatttgact gtgatgatac aaatccttct gtgagcgtac atggaacaat gttgcaagcc
    15361 ggttctgtgg aatttcgcaa agggcaccgc tacgttggcg tgcggtttgc acccggcttt
    15421 gttcctgatt ttttaaagct ctccgcggag gaattgattg aaggctcttt cgatcttctt
    15481 gacgttagcg ttaatgctga acgcgtattt gagcaggtcg tacgtgagaa gaactttgta
    15541 acgcaactaa cttttcttaa gcagtttttc ttgaccaata tagctccgcg caaatcatta
    15601 aagctgacgc ttccagtcgt caatatgatt tgccaagccc aaggaaatgt gtgtatcaaa
    15661 aacctggagt cgctaacggg ctacaccagt agaacgttgc agcgctcctt tcacaatgat
    15721 ttaggaatga caccaaaaat gttttgtcga attattcgct gccagtctgc gatttataaa
    15781 attcatcaca acaatattgg ctttagcaat ttagcaattg acttgggatt cagtgatcag
    15841 cctcactttt taagagaatt caaacatttt gtcagtgcaa cgccgctgga ctataaaacc
    15901 cgtattctga aggaaaactt ctttagcaga attcacttct attaaaagcg gtcggcgtcg
    15961 cccagcaatt atcccgctcc attatttagc agcaaggctt ttactgcctt ggccgagcca
    16021 ctatcgccaa aggcaccaag cccaacctac cgacttagtt atggagatat tgcgagttca
    16081 cccgctgcag aacgggctcg gctactgaga cgctgcaatg gaagagacgc tgtacgagcc
    16141 ccctccgctt tcaggcgagt tatcgcctca ctttaagcac tcgctcgaga actgcgccta
    16201 aggcacagag ctgttcatcc tgatgcttcg ggccaatcaa ctgtagaccc gccttaattt
    16261 cactgccttt gcactgaacg ggcacagtga gtgcgggatg ccccgaaaga ttgaaaggtc
    16321 gcaggaacgc tgtcatatcc aggattgatg cgcccgctcg aacggactcc actgagggtg
    16381 gcaggcgcgg taggctgggc atgaccaaag cgtcgaacac ttttagttgc tcatccactt
    16441 ctaagcaaaa ttgctcacga actagctcag cggcagtaac ttcatcagcg gtcgttcgac
    16501 tagccgccag taaacgtgcc tccacatcgc gccccaacaa tccttgtccg gtatattgtc
    16561 caaacgcagc ccaggtttca tggttgatca gtaccatcgc tgcattgaag gcctgcgcca
    16621 ggctcggcaa tttcactgcc tggcatgtcc acgatgtagc gctgagtgcg tcgtaaactg
    16681 cctcacttac aatgccgtcg cactcaactt ccacgacccc aacccggtat tctgcgcgtg
    16741 gggttgcagg cataaatgaa ggatcaattg cggtcatcac atcgactatg cccgccatct
    16801 ccctggcgaa aggtccaatg caatctaaag tgctatggac agggtaagca cctgctcggc
    16861 tgacgcgtgc gaatgttggc ttaaagccga aaaccccaca gcaagccgca ggcatccgca
    16921 ctgaaccgcc cgtgtcagtt ccaatcgatg cgtagacggc tcccgccgcc actgcaacgg
    16981 cggagccgct cgaggatccc cctggaataa ggtccttggc ttgagggtta atcggcgttc
    17041 ctgcccaatc gttaatccca ctgacaccaa aagcgaattc gtgcaggacg gttttaccga
    17101 cgatctgcca gcccgactca agcaacagtc ttacaacatc cgcatctttt atcgcaggca
    17161 agttatctgc cagcgcacgg ctacctgctt gggtaaccca tcctgagacg tcgatagagt
    17221 ctttgatagc aatggtgggg ccatcttgat ggcctatgct tttttggatg acaaacggat
    17281 tcatgggacg tctcaggcta gggtcaaagg cgagctcaat agacgttcgg tctgaaactg
    17341 gctaatcagc caaacgccat tgattcggat gaagtggata tccaagcgtg ccaggatcag
    17401 atcggcactg ccctcttcat agtcggacag ctgttgcatc agccaactgc cgcgagcctg
    17461 atcgccctca acgtcgatat gttcagagcc taggagatgg ccattggtac gaaagtgagg
    17521 cgacggcggt aaatagctca tcaacatatt gatgatcgcc gctcgccctc tgagcacacc
    17581 gaatttcgac gtgtataggc ggccgactcc ctgccagaca gcgtccggac taaaaagccc
    17641 ccccagagct tcagcaactc cttcaggctg tacacagggt acatcgcaca gctgcatata
    17701 gcgggtgaac agtcggcgga tagcagattc cgattccaat ctgtcgaggc gagcatgcag
    17761 ctgaagaaag ccgtcattta gatcgattgt aggcatagtg gtggcgccag aagttagtag
    17821 tctctcccaa gactcgcgca tcgaatccag cacctcaata accgaaaatt tatttcttgt
    17881 tgcacgagga tctgttcccg tttcgctcgc ggccgcgccg gagccagttt tagcgcgagg
    17941 ccgtgtgccg tgttatagat ggcaaccatt gaaatgggta cgctggcaca accaccagcg
    18001 cctgctgggc tcaatcggat ctatctcgcc tgcggaggct gaggcaaacg tccaccagca
    18061 acaagcaggt caggccatgg cagcctgacc taaacgaaac ggcctccaca aaagccagtg
    18121 cgattaattg tccgaatggc gacctgcccc tgctccgcga tgctgagcta tctcccgttg
    18181 cggcggtaac gacgataaag gcctcctggc gtcatgctgg agaaaacccc gtcacggcgc
    18241 acccaggcat gcatgagtgc ggctgaaaga tgcatcagca cgacggcgaa cagcagccag
    18301 gccagcaggc catgggcatc acgcagcacc gcgtaccaga ccggatgggt aggtgcgatg
    18361 ggcggcagcg tgagacccga aaacatcgtc acaggccatc cgcccgccga gcgcacaccc
    18421 cagccgagca gcggtagcga gaacatcagc gcatacagca gccaatgcga tgcattggct
    18481 gatatcactt gccaacgtgg gatgtcggcg ggcaacgcag gtgcaccgtg acgcagccgg
    18541 ttgaccaagc gcacgatcac caacagcaaa atggcaatgc ccaacggttg gtgaagatcg
    18601 agcagctctg gccgcaaggt cagcgacgtc atcatggcca cgccgatgaa cagcatcgcc
    18661 agaattgctg ccgccatcgt ccaatgcagc agctgcgcca gcaaattgaa ccgttctggc
    18721 aggccagctt cattgccttg cgaatctttc gttaaggtgc gggtttcgat agtcattgtg
    18781 ctgtcttctt gccaactgca tcggtggcct tgtcgtagcc gatttcgtgc agccgtgagt
    18841 tgtacgagtg ggcataaata cctgcccgcg tggccaatac cggatcgttt gatagcgcca
    18901 tccctgccgg tatcaaggtg ggatcgaaat tgatgtcgcg acacacgccg tcagtctgat
    18961 caaagacccg ctgcacttcc aacgttccgg cgactacctg ttggcgggtt tccggccaag
    19021 gctgcgatgg atcatccacc ggatcacctg gtgcggccag ttgcatcacc atatcccaat
    19081 gtaacggcgc ctttgtcagg cgttgcgcta cctccttgaa caaggcgtca tgactggccc
    19141 ccgcacgctc atcatcactc caggcggtga atgtcgcgtg cggccgcagc caccagcgca
    19201 tggcttgccg tcgcccatcc tcggcgacca gaaagtaggc gtgaatggcg tagtacgtgg
    19261 cgccggcaaa gctgctggcc cagggtttca cggcatcggc ctccatgtat ttgcgcgcct
    19321 ccggatactc ggcgaggaaa gctgccagct gctgggggtt ggcctttccg gtggtcggat
    19381 caggcgcagt ggccttcatc atctcgagga agccgggggc atcgcgggtg gggaaatacg
    19441 gttcgttgtt cattttcatg cgccactgct gcccgttatc ggcagtgagc atcatggcca
    19501 tgctgacggt gctggtggag ttgtccgcgg cataggggct catggtacca atcgagaaac
    19561 gtcctatggc ccgtgtctcc ggctgagcga aaacgcgggc atgggagtac tttgccgccg
    19621 cgccgctgga gcgaaacacc ccttcgaaac acattccctt gccgtgcgcg cgtcggtaac
    19681 cagcaggaaa accatgctgg gtgtcctcca ggaaggtcgt attggtcacg cgctggccga
    19741 gcaagtcgga agtccaggcg aaggccgtgg ccagcacgcc gacgatcgcg ccgatgccgg
    19801 ccagcgccgg ccagctccgc ttcaggtcaa tatcgccgat cttcatggtc ggcctccgtt
    19861 gcggcgtcca ttggtggaca gggcgagtgt ggtgagggat gtgttgctca tcatgtctcc
    19921 ttgaatagat aaaatggctc agagaaaatc tgcggggtgc caactccgaa tcacgatcac
    19981 acccgtcaca ataaaagcga tgccgatgat tgctggaaga taagatccag tctttgctta
    20041 aaaacaacta ctccgaccag cgtggccaag acgatgccta aaccggccca tatggcgtat
    20101 atgatcccga gcggccaacc tttcagtgcc tgggcgagtc cgtaaatgca aatccgtaaa
    20161 aacaatcacc agcccggttg gcaccaggcg ggaagctgtt cggacctagc ccatatagga
    20221 tgttgcaagc atgttcttct cttgttttct gcggttattt ttgcgcatgg catttttttg
    20281 ccatcattgc ttcgcagggc ggctttatac aatacgatcg tatcgtacta tattttttgc
    20341 gttgtagaat ccaggtatga cctctaaagc gaacgcttcc cggcctccga aacaacccga
    20401 ccaggtcaga aaaaggcttt tccacgccgc cactgtcttg ttaagtactg ggcagcccct
    20461 ttctatcggg gcaattgcca gtgcagccgg tgtcagcaaa ggtgctgtac agcatcattt
    20521 cggtacaaaa gaacagctgc ttgctgcagt tttcgatctt tatatggctc aattcaacga
    20581 agcactggcg ctggaagatg cgtcggctcc gccagccttg cgctatgcgc gcatgtcgct
    20641 caatgccact tcgtatgaag acaccgatgg atggcgggca atggtagtgg ccactgtcat
    20701 agagcgcgga ctcgccgcgc ggtggagtga acgagcagaa attgagcgct ctctcgacca
    20761 acctccgtct gcgaatgcct tgctcgtgcg tttggcggca gacgggttat ggctatcgga
    20821 tttgctgtct acctataaga tcagcaacaa agagcgcgag gaacttgatg tcttgatgca
    20881 ggaactgctc acggatcctt taatcaatgc catccgtccc tgaggaatcc ccacaaatct
    20941 actccagcgc ctgagcttgc cgctgaacaa tttaaatccg taatccagct tcgatgggag
    21001 gggccaagtg cgcgaatcgc aagccggaca gacgcttgtg cctccgtctg caactaaggg
    21061 gcagcatcgt ttttagcctt ctgatacctc catcgcttac taattgggtt ttacagattc
    21121 cgcccgttgc agccgttccc tcaaaaagca ctttgaactg aagcctaaaa atactttcgt
    21181 gcgcatgccc tgcgcggtgc tgcctgacca ctgaaaaaat aacgacttga cctggcctcc
    21241 gggttctcgt taacttcgcg cgcccctacc ttccggtagg aagctaatcg tgagtttgtt
    21301 atggaaaaga gtcattccgg caatccttcg ctgaagtttg ccattgcctt gggcgccatc
    21361 gccgcactgg gcccctcagc cgtcgatatg tatttgccaa gcctgcccga aatggcagtc
    21421 gagtattcga ccagtgttgc cagtattcag ctgacgctca cggtcttttt gttggcaatg
    21481 gggcttgggc aattgctgtt cgggccggtt gtcgacgcct tgggccgtcg acgtccactg
    21541 atggcaggct tgttgctgtt cagcttgagt gcgctgtccg caggcgcggc accgaccctt
    21601 gaatggctac ttgtggcacg atttgcccaa gggataggca gtgccttgac gttggtcgtc
    21661 atcatgagca tggtgcgtga cgtcacgcag ggcgttcagg cagcaaagct ttttggcctg
    21721 ctgctgacca ttcaaggtgt tgctcccatc atcgccccag ggttgggtgg gtacattgat
    21781 gcacattacg gatggcgggc cgtcatgctg gtactggctg ctctcggtgc gctggtggcg
    21841 gtgaactcga tggctgttct gcctgaaaca ctgcccgtgc aaaagcgcga gccattgaat
    21901 atgagacagg cgctccgcac gtatgcgcgc atcgcacaag acagtcgttt tctaaagccc
    21961 accttagcgc tgtccgccgt gttctttttt ctctttgcct atgtaggcgg cgctacattt
    22021 gtttatcaga atgaatacgg actgcctact ggccagtttg gtacgctgtt tggcgcaacc
    22081 ggcatagcga ttcttatcgg ggccgtcgtg gctacccggc tggtgggcaa gtacggtttg
    22141 gcccgcatgg caacgactgg agctttttgc atctttgcag gctctgtcat ggcctacggc
    22201 gcagcggtat ctggttctga cttgtactgg ctggccgccg gtgtcgcgat agcactgctt
    22261 ggattgggga ttgcagaaac ctcattgatg accctggtga tgtcttcgca aaccactgcg
    22321 ctgggttcaa cggctgcatt gctgggcgct tttcagcttg gcatctcggc cttcgccaca
    22381 ccgttgaccg gttgggcgag cagccatggc gccattgcct ggtgcgcatt cctgatcgtc
    22441 tgcgccgttc tggtgggcgc gctgacctgg gtcagtgtgc gccagggcag agccgagcac
    22501 atcaccgcgt tgaatcactg attaaaagga catcctgatg aaagtgctga gcccttcggc
    22561 aggcagaatc tgtgacatcg ccctggtgca tttcgctgag cgtggatacg acgcctcgtc
    22621 gctcaatgac attgctgtcg tggcaggtat gcgcaagcct tcgttatacg cgcacttcaa
    22681 aagcaaggat gatctgttcg acgccgtgct ggatctggcg ttggtgacgg agagccacta
    22741 tatcgaagag atgtttgcaa gcgtcccggc gaataatgac gagcccggca agctgtacac
    22801 ggacagcctt tcaaaccgct acgagtcttc cgcggctctg cgtttcctgc tgcgcacggc
    22861 gttctttccg ccttcggagc tgaaggcatc ggttacgaga ggctttgaag cctatcttga
    22921 tcgtctgcgt gaactctttg gcgcaagcct tgagagcagg tatccagccc tgtcggggaa
    22981 cgagatttcg atgtttcagg atgcttatat cggggttatc gacagccttc atgtcgagtt
    23041 gatttattgt agtgaaggaa cctaccgtcg gcgtcttgcc gctttgtggc gcattcttgg
    23101 tgattcgctg tctctggctg tggggaaggt ggcacatgga taactcaacc attcaaacgg
    23161 tacaaaacgt agcgagcgag ttaaacgtta ttcgaactag cacgacaatc aattccatgg
    23221 agatcacaaa atgaagcttt actacaagcc tggcgcctgc tccctttcgc cacatatcgt
    23281 tgcaaacgaa cttggcctca atgtcgaatt catcaaggta gacctcaaag atcatgtaac
    23341 agaacaaggg aagaattttt acaaggtcaa cccgcacggc tacatacctg cgcttgagct
    23401 ggataatggt gaaatgctct tggaaggcgt tgtaatcatt caatatcttg cggaccagaa
    23461 gcccgaaata ggccttgctc cagccaacgg gacgtttgaa agatacaagc ttcaggagat
    23521 gctcgccttc ctctcaacgg aaatccataa gggctttatt ccgctctttt atgctgctcc
    23581 ggcaggagat tacatcgaga ttgcaagacc caagttggag aaacgctacg cttggattaa
    23641 tgaacatctc tcagacaaac agtttcttat gggtgacaaa ttcacggtcg ctgacaccta
    23701 cctgtttgcg gtgactggct gggctaaggc gtcctggcta aagtcctacg aggacacgcc
    23761 acttcatctt gacagtcttc atcatctgca gggttggtac gagcgcgtga agagccgtga
    23821 agccgtgcaa cagtccattc tcgaggaagg tctcgacttg aactagcgtc ttgatgcccg
    23881 ttgccaacgg gcacccaact gctctgaata ttgtctggat ctctgatgaa cctgcgcgcg
    23941 atccttattc tcggtgcctt gagcgccttc ggccccttgg ccgtcgactt ttatctgcct
    24001 ggcttcccgg ccattgcctt ggcgtttggc actgacgaga agcatgtcca gttgaccctg
    24061 gcgtcatatt ttctgggctt gtccatcggc cagctggctt acgggccggt ggcggatcgc
    24121 ttcggccgac gtatcccgtt actgttcgga gcttcgctgt tcaccctggc atcgctggca
    24181 tgtgccttcg caccaaacct ggaatggctg atcagcgcgc gcttcgttca ggcgctgggc
    24241 ggctgtgcag gtatggtaat tgcacgagcc attgttagcg acaagtgcga tgccgttcag
    24301 tcggccaaag tgttctcgca attgatgctg gtgatgggct tggcgccgat tctggcgccc
    24361 atgctcggcg gcttgctggt caacctctat gggtggcagt cgatcttcat cagcctgacg
    24421 gcgttcagtg cactggcttc attggccgtg gccttgggcc tgccggaaag catgcccgcc
    24481 actcatccgc gcctgccttt atctggcgca ctgacgcagt atcggcgctt gttgggcgat
    24541 cgtatttttc tcgggcacgc gctcactggc gggatcgcca tggccgggat gttttcttac
    24601 atcgccggtt cgccgttcgt attcatcaag ctgtacgggg tgccgcctga gcactatggc
    24661 tggctgttcg ggaccaatgc ggcgggcttc atcctggtct cgcagttcaa tgccaggttg
    24721 gtgggcaaaa aggggcctgc gttcctgctg tcgagggtta tctgggtgta cctcgctgct
    24781 gcactggcat tgttggcgat tgccggtcta cgcaccgaac agctctggcc cctgctagtg
    24841 cccttgttta tctgcattgc ctgcctgggc tccatcgccc ccaatgcctc agcctgcgcc
    24901 atgaacgggc aatgggcgcg tgcgggcagc gcgtcggcat tgtttggttg cctgcagttc
    24961 agcgtggcgg cagcagcggc cgcgctggtg ggtgtgcttc acgatggcac ggcgttgcca
    25021 atggcgctgg tcatcagctc gtgcggtgcg ttggccgtga cgcttgcaat gctgacccgc
    25081 cgtttgcagc acaaacgtga tgtggcgctc ccgggttagt cggagattac tcgacgcttc
    25141 accgccgact aacaaaacct gtacctaccc cgatgggcaa tctgcccttc agggcaagtt
    25201 cgccctcatg tcgagagcga ccaatgtcca atctcacttc atcgccactg tttcgcatcg
    25261 acgagtttcc tgacctttac gtaaagacaa caaacccgac tgttgctgtc attgtggcca
    25321 ccccacagct gagagaaatt aaccccagta gggcctcagt acaagaaaag gtccatccga
    25381 acctcaacaa taggcattcg ctcatggcat tggcaaacaa ctacgacaac ctttccgccg
    25441 gcgctcaatc cgcaatcggc aagcgtactc agctgttcat caatggtgaa ttcaggaatc
    25501 ccctttcagg gcagtatttc cctatcgttg atcccagtaa cggccttgag cttgccgagg
    25561 ttgctcaggc ggacaagcaa gatgccgact aagctgtctc ttcagaacgt aaagcatttg
    25621 aagacagtac ctggtctaag atgaagcctt atcagcgcga agcgctgatg ctccggctcg
    25681 ctgatctgct agctgaaaat gctcaggaac tggctgaact cgagtgtctc agtccaactg
    25741 gtgctcattc gcacgcagct tcccaaacaa gagaatccca tgcttgatac cctttccatc
    25801 cgcctaaaaa taattcttct ttccggcttg tgcctcttgg gcgtgattgg ccttatcgtc
    25861 agcctcaatc tctatcaaag cagccagaac aatcagttga tcaggacgtc gagcagcaag
    25921 atgctgacgg aaagcggaga aaacctgttg caggccaagg ccgccgagca ggcaagcaag
    25981 ctgcaaagta ccttcggcga tagcctgctg atcctcaccg tcctttccga ccaggtcgcc
    26041 aacttgcgca atctggccgc ccagcgtggc ctggcgcccg gcgcattgcg tgaagaactg
    26101 actcagagcc tcaagaccac ctttgatcac aatccgcagt tattagggct ttggctgctt
    26161 tacgagccca atgcactgga cggtcaagac agtgagttcc ttaacgatgc cgcgcgtagc
    26221 tccaatgaca aaggacgttt taccagcggc tggaaccggg gggcgggtca gtcggtcaac
    26281 ctcacgattt ccgaaaacgt tataaacaat accgagctga gcatcagcgg cgtaccttac
    26341 aacagttggt acacctgccc gcgagacaat caacgcacct gcctgatggc gccctatgtc
    26401 gagaccttgg ggggaacggc acagctgatg accagcctgt ccatgccctt aattgtcgat
    26461 gacaaggtca tcggcgtgat tggcgtggac ctcgcactcg gggctctgca agcggcagtc
    26521 agcgatgccc agcagacgct gttcaatggc gcgggcagtc tgctgattgt ttccgacagt
    26581 ggtgtgctcg ccgcctacag caaggatgca ggtcagatag gcaaacacat cgacactgcc
    26641 ttgggcgagg cgagcaaacc agtgctcagc ggattgcaac tcaacaagcc ggtggtgctg
    26701 actcaagacg aattgatccg cgcggtctac cctgttgcgc ccatattggg cgcagcggtg
    26761 tggggggtgg tgatcgacct gccgaagcag gtattgctgg ccgactcggt agaacttcag
    26821 accttgcttg accagacaca aacccgcgac accctgaagt cgctgctggt agcagtatgc
    26881 gctgggctgc tgggtctgct gatcatctgg ctcaccgcgt ctggcatcac gcgccccatc
    26941 aatagtgtcg cccagatgct caaggcgatt gccagcggtg acggcgacct gacccagcgg
    27001 ctgaactaca gcaaaaaaga cgaactgggc gaactggtga actggttcaa ccgtttcttg
    27061 gacaaactgc agcccaccat cgcccagata aagcagagca ttaccgacgc cagaggcacc
    27121 gccgaccagt cttcggaaat cgccaggcag accagtgaag ggatgcaggt gcaatttcgt
    27181 gagatcgatc agttggcaac agcctccaac gaaatgagtg cgacggccca tgaagtcgcg
    27241 cacagcgcct cgaatgccgc acaggccgcg cgaggtgccg acgaagcgtc aagagacggc
    27301 attaccctca tcgaaagaag cactcgcgac atcaacgcct tggctagcga ggtcagcaag
    27361 gccgtcgcgg aagtcgaagc tttggcggtc aacagcgagc aaatcggatc cgtgctggaa
    27421 gtcatccgca gcatcgccga acaaaccaac ctgctggcac tcaatgctgc gattgaagcc
    27481 gcgcgggccg gagaaagcgg tcgtggtttt gcggtggttg cagatgaagt gcgcaacctt
    27541 gccaaaagaa cccaggactc ggtcgaacaa attcgactgg tcatcgagcg tatccagacc
    27601 ggcacgcgtg gcgtggtggc aaccatgcat tcaagccaga cgctggccaa gaataacgcc
    27661 gaacagatcc aacaggcggt acaggccctc ggcaaaatca gcgatgcggt gaccgtgatc
    27721 agtgatatga atctgcagat tgcaagtgcg gcggaggagc aaagctctgt ggccgaagag
    27781 gtcaaccgca atgtctcgaa cattcgcggt gtaaccgagg ctctgactgg acaagcggct
    27841 gaagcggcga aggtcagcag tcagctcaac gcccgggcca atcaacaaat ggtgctgatg
    27901 gatcagttcc gcgtatgaca caaagccggc ttcgcttcag tgatacagcc atcctgcggc
    27961 gcagtcgata tttctactat tgcgaccgcg tttgatcgtg tccctgtaag gcgcctgagc
    28021 acgaacaagg gcagccgaag ctgcccttgg ggatcacact cactgctatc cccggtaggt
    28081 tactaagctt cggcatggtc cagtatggat acgcgctgtg aatgggcgca gggcgaggcc
    28141 agatgcgttg ccccagcccg cgctttgccg caggcttagg cgcccccttc gctcctacag
    28201 agagcgacat attatttgtg tcgcgacact aaggatggtc tgaagtagcc ctgtatttcc
    28261 ggccgacttc agcccctgcc gattttgaaa gcggcaaacc gatcaaaaac gatcagatct
    28321 cccgctcatt tcgatgtttc tggccgctgc gataacctcg ccgtagccat ttcccacatt
    28381 acaggcgcac ctctcctgca ttcgtcagta aatgctgagg cgccatccac agattcgata
    28441 gcgcaaacaa cgtcaccagt tgtgcggtgt ttttcaccag cccgcggaag cgcaccttgg
    28501 tatagccaaa ctgccgtttg atcacacgga atgggtgctc gaccttggcg cgtacctgcg
    28561 ccttggcctt ctcgatcttg cgtttggctt tgtacagcgc gctgcgctta ctcagcttct
    28621 tgtacgtact gcggcgggca gcgatctgcc agatcacctc ccgaccgtca tgctctgggc
    28681 gcttctctac gccggtgtaa cccgcgtcgg caccgaccat gttttcctcg ccgtgcagca
    28741 acttgtcgac ctgggtaacg tctgcaacat tggctgccgt gccgaccacg ctgtgcacta
    28801 aacccgattc gtcatccacg ccgatgtggg ccttcatgcc gaagtaatac tggttgccct
    28861 tcttggtctg gtgcatttcc gggtcacgct tgccgtcctt gttcttggtc gaactcggcg
    28921 cattaatcag cgtggcatcg acgatggtgc cttgccgcaa tgacaagcct cgatcaccca
    28981 ggtagccatt gatcacggcc aggattccgg tagccagttc gtgtttctcc agcagccggc
    29041 ggaagttgag gatggtggtt tcgtccggaa cacgctccag gctcaacccg gaaaactggc
    29101 gcaggatcgt cgtctcgtac agcgcttcgt ccatcgctgg gtcgctgtag ccgaaccagt
    29161 tttgcatcag atgaacccgc agcattgcca tcagtggata ggctggacgt ccgccttccc
    29221 ccttggggta atgtggatcg atcaaggcaa tcaaaccctt ccacggcaca acctgatcca
    29281 tctcgatcag gaatagttcc tagcgggtct gcttgcgctt gccggcgtac tcggtatcgg
    29341 cgaaggtcat ctgcttcatc ggaaaactcg gcgggtggag tcaggggatt ttgccaaaat
    29401 caggaagtct ttttcagtgt gtccctagta aatatcgacg tgccatccgc atgctagaaa
    29461 acgcaaactg tgcttccagc ttgccggcac acctggtcag accaatcggc actgccctct
    29521 tcatagtcgg acacctgttg catcagccaa ctgccgcggg cctgatcgcc ttcaacgtcg
    29581 atatgttctg agcctaggag atgtccatag gtacgaaggt gaggcgacgg cggtaaatag
    29641 ctcatcaaca tattgatgat cgccgtgcgc cctctgaaca cgccgaattt cgacgtgtat
    29701 aggcggccga cttcctgcca gacagcgtcc ggactgaata gcccgcccag agattcagca
    29761 acgccttcag gctgtacaca gggtacatcg cacaactgca tatagcggct gaacaatcgg
    29821 cggatagcag attccgactc caatctgtcg aggcgagcat gcaactgaag aaagccgtca
    29881 tttagatcga ttgcaggcat ggtggtagcg ccaggagtga gtagtatctg ccaagactcg
    29941 cgcatcgaat ccagcacctc aatgatcgaa aatttatttc ttcttgcact agggtctgtt
    30001 cccgtttcgt tcgcggccgc gccggagtca gttttagcgc gaggcaaggc acgagatgcg
    30061 aagtttagcc agctaaatga gccatcgaga aacgcagcat cgcgctaaaa cgggcccggc
    30121 ccttcgggtt gcgacgaaac gggaacagcc cctaaagaca atggatttcc cgccgcgtca
    30181 ggagccgttg gtcatcagcg cgccatgcac gggcgagaac aaattcttga tcacgccgga
    30241 acgccggcga acggcccatg tcgagcgcat gttttccgcg catcggcccg ggcgttgtca
    30301 tatcggcgct cgcacaccgc attgagctga ggctcgacct gctcgatacg cgctatcacc
    30361 gccttcagtc attcgtcggg ctgtacttcg ccctgacgca cccgctcgac aagcccacac
    30421 ccgtcgtgcc tgtccagcaa cgtatgaatg tcgcgcacaa cccaaaatct ccctgagtca
    30481 ggagtggctg gacatcggtt gccctacagc gcgtttgcgc tgcaccaggc tgaccacgag
    30541 ccagagggaa gctgccacca tactggcaat cgccagccag tgaatcgatg cgcgcaagtc
    30601 gttcgtgaaa gcgccaacca gtgccactgc cagcgggctt acgaactggc cgatgtagag
    30661 acaggcagtg aacacaccga ggccgcgccc ccgggtgttg gcgctcagcg cgttcatgac
    30721 cggcgccatg gcgttgggta ccagcaagcc tgcgcccaag ccgtgtacgc tgacggccag
    30781 cagaatttcg ttgtagcttt gcgcccgagt cagcaaccac aagcccagcg ccagcaaggc
    30841 cagcagcaca acattacaac cggcgatgcc taccccacgg cgcagcagtg gccagatcag
    30901 cgaaccacct agcgtggcta gcagacccag cccggcggac aagccgatca tggtgctgga
    30961 actgatgccc aactccacca gtagtatcgg cgcctgtacc ggcacgatca ggctgagaat
    31021 catcccgccg aggatcagca gatagccact caccaaggta gagatcagca cctgctcgtc
    31081 atcctcgttg actgccgtct ctacccgcgc gcgaacgggc tcccacaaca cctttatcat
    31141 ggctggcagc agcaacagcg gcagcaggta aaggtagaac ggcagtcgcc atgactgttc
    31201 gcccagcacc ccgcccacca cgaagaacag cgccccaacc aggccgatgg tgaccacctg
    31261 aagattgata tagcgcatgc gctcctcccc ctgccaatag tcggcgatca aggcggcgca
    31321 acaggtcatg atcgccgctt cggcgcagcc gaacagcaag cgaataccga cgatggctgt
    31381 cagatcattg agcaaggccg gcactatgcc gagcagcgca taaagcagcg tcgcagccac
    31441 cagaagagct ttgcgcccca cccgatcagc taaccagcca gccaacgggg cacacaacgc
    31501 aatcgccagg gccggaccgg tcacagccag gggcaccagc aagtcagcgt gcggctcggt
    31561 ggggccgaac tcggctgcca gtttgggcag tatgggggca accatcaccg cccccatgat
    31621 ggtcagactg ctgccgagca tcagcaccac gccttcgcgc cgaccagtgg cctgacgtat
    31681 gcgtggcgtc gcagcgttag caatggaatg ttctacggac atgcgcaaga cctcgataaa
    31741 acaattggga tactcagagc atggaagcgc ccaccgcctc agccctcgaa aagctccatg
    31801 cctggcgtgc ccggcagggc aacgctaccg acgcttactg agcaaagcgg catcgccgct
    31861 gaaacgctgg caaagacacg ctggcagctt cttctgatcg gtttgacgga agaatcctag
    31921 ggcgcgattc agaccgaccg ttagccaaat tcggcagaat caccgtgcct ggctaacgcc
    31981 aatatcgccg acctgcccca cggacaaagc cactgataca cacgggtgta gccgaacagc
    32041 tggcggtata ggcgtccatg gtgggtgcaa caggcagcgt ggtgtgctgg gaaaacgcct
    32101 tgtcgcaaaa tcgtgttgca gccacagaca gcaccaaccg acgcctcggc aggcccggtt
    32161 cagagaaaca gtgtcgcaac caactcggtg cggttgctca cggcgacctt gcgaaacaga
    32221 ttgatcaaat gcgttttcac cgtaggcagc ccgacaccca gctggcgcgc gattgccttg
    32281 ttgcccagac cggcacgcag gagctcggcc acttcccatt cccgtggggt cagctgctgc
    32341 gcgggcgcag agggagagag ctgtttcacg gcgatttcaa acataccctg cagcgcctgc
    32401 aattgaacca gctcatgtgc accgaacaga ccgagatcac ggccccgcag aagcgagata
    32461 cctgcgatgg gtaacccctg ctcgaaagcc agcacttcga cgacgtcgac gactccaaag
    32521 ccctgtagga aatctccata gcgctgattg gccgtatcgt tctgcgcacg catgcccagc
    32581 cgcagcggca caacgctaca gccggtcagt tggcaggtac tcggctgcaa agggtcgagc
    32641 cggcggtagt acgacagata gcgctcatgc atgtccgaac gcatctgctg gaggaggaag
    32701 tcgtgtggct cgagctgcgc agcgatacgg taaaacacgc tacccgaagc gggtatgagc
    32761 tgcgtgaagg tatccagaca gcgccgccca agttcagcac tttgcataga aagatcactc
    32821 gataggcccc tgacgaccgc actggctgaa aggggcgaga cgaagaacct acacgaccgc
    32881 caggtagtgc ttggtgaagc tgtcactgag cacgtcccag agcacggcgg tacccttggt
    32941 cacgaaccag ctgtcgccgg ctgtataccg ggtgctctgg cctgtgctct cgtcggtcat
    33001 gatgacttcg ccgaagacca cgacggcctg ctcgttgaat gggtagacca tacgaaattt
    33061 actcttgctc acggcgaagt agccagcgct gatcgcgtca gtcggagcac cgaaggtcat
    33121 ccggccgaac gccttgccct cgccctcgag aatttccgaa cccagatcgg ctacgctacc
    33181 ccatgcgtcg agctcagtga aagtggtgcc ttgcttgata ggtgtcatat agcctttcct
    33241 ctgaaaatgc agtgcgtgga taaataagta gttagcgccg cccgctccag taaccggaca
    33301 actggtgcca ggatttgccg gccgtcagta gcagcggacg gatttgatct ttgccgagaa
    33361 ccgggatacg acgcacagag ctgatcaggt cgtagcgtgc tgaaccttcg ctcatgccct
    33421 cggccagcac cttacagatg atgtggctgg gcgtgacgcc aaaccccgaa tagccctgga
    33481 cgaagaacac gttcggatgc tgcggcaacg aaccgagctg cgggaataga ttggggctgc
    33541 aggccatggg cccgccccag gccaggtcga tcttcacatc cttgagatag gggaagatct
    33601 tcagcatcag gttgcggttc caggccttca gatcgcgggg aatgtgctcg acaaaaggag
    33661 tggctgcacc gaacagcaag cggttttccc tagtaacccg gtaatagtcg attaccgggc
    33721 ggatatcgct gtaggccccg cgaatcgggc tgatacgctc gacaagctca tccgacagcg
    33781 gctcggtcat gatctggaag gcgtaggtgt tgatggtgct cttgtgcagc tcgggctcca
    33841 tcttgttgag aaagctgtcg caggcccata gcagtttgct ggccttgacc gaacctttgg
    33901 ccgtacgcac ggtgacgcgc tcgccgtagc tcacttcaag cgccgggctg tattcgaaga
    33961 ttttcacccc atgactcgcc agagccttgg cttcgccaag cagtagattg agcgagtgca
    34021 cgtgcccagc gcccatatgc aacaaaccgc tggagtatgc gtcagagcca atcatttgct
    34081 tcagttccga gccaaccagt aggcggatct cgtgctgcgg gctcagcgcc ttaaactcct
    34141 tctcccaggc tgccagggtc ttctcctgac gagcgttgaa gcccatatag ccgtagccgt
    34201 gacagaagtc ggcatcgatg ttgtaacgct cgatacggct tttgatgatt tcggcgcctt
    34261 ggtcgctgat ctcgaaaatg gcgcgtacac cctcctcacc tacgtccttg cgaatcttct
    34321 ccaggtcatg accgatgccc gccatgatct gcccgccgtt gcggccagta ccaccgaagc
    34381 caaggtaacg tccctccagc acgacaatat tggtaatgcc ctgctccgcc agctccaaag
    34441 cggtgttgat accggaaaac ccgccgccaa tcacgacgac atccgcttcg atatccgctt
    34501 ccagagaagg aaagctcagg tcgtatttct tggtggccgt gtagtaggta ggcgtttcaa
    34561 tattgatcat gcgcagcagc ctgagaattc aaaagggaac gcgatcagga agactggctc
    34621 tccgaaaaaa ccgcgttctg cttgctcaca ttaagtacgg accggcctgc ctgcgtcttg
    34681 cttgtggctg cctttccact tgactgcttc tgccatcgct taccagcaac gaatagtgga
    34741 tcgaaaaaga taaacatgtt aaccgttagc cattaaccat cgaaaacgcc tacttacccg
    34801 gtatagggca aacccgccgc ttctcgccta ttcaaaggtt ggcagtgatg gtcaaagaac
    34861 aggcaggctt gaacaagaca gttattcgac ttaccttcgt aataggagcg tgactagcac
    34921 taggccatct gccgctggcc tgccgtgacg ccaccccgag agcctgcctt cgtttgcccg
    34981 tacccgctca aacgctggcc tgccacagtc gtgccacaag ccgaactgag aggctgctta
    35041 ctaaagaaat cttcaacaaa aacaataaag aaattttcct tatgcagccc aggtgtcccc
    35101 ccgttgggtg gactgtttgg tgttgactgg tgaccaccca ggttcaggcc agaggcaggg
    35161 atagccacag acgtgacacc gcagcatgaa cactgcgaat gtcgcaccac cggctggagc
    35221 atgacgctca tcagaaagca ccgcaccgac agcgtcgagc ggattgttat gagcacttat
    35281 gatgtcgtag attcaacctt ccaagcccct tccaccgccc tatccagcta ctcccggcac
    35341 gtaagctcca acgatataga tgaacatgcc gcccatgtga gtggctggca cgtgaactac
    35401 gatcaaatga gcgccggcct gtttcaaggc gagctgagcg agttcaagct ggactggatg
    35461 caggtgatgc gcgaccgcac caatcagtct ctgctcaaga agggctcggc gaagtcagga
    35521 acaatcacct tcagcgttcc cgtcagctac gccggcaatt gctattgcgg cggccatccc
    35581 atgcaagacg gcaatgcgct ggtggcacac gccgatgacc ttccccaact gcgcacccca
    35641 gatcagatgg accttatcgt cgtggcggtg gagcaggaga ttctcgagcg cgagctggag
    35701 ttgcacggca ttacctttga cagcgcacac ctgccgaaag tctatcaatt gcaggggctg
    35761 gcactcccca ataacctgcg gatgattctc gatgatctgg agcgcggtgt tgccctgcca
    35821 ggccttcctg atgaggtgtg gcggcaccag gttcgcgatg caatcatggg gcagatactt
    35881 gatctgctgc cctccgtgca gcctatcgag ctcacgccct cagcccgcaa acgcaccgtt
    35941 gatcgcgcct gcgagtacgc cctggcccac ctcgatgagc cgctgagcat cctcgatatg
    36001 tgccgcaccc tgggcgctag ccgccgcaag ctgcagtact gctttcagga gacgctggga
    36061 accaatccgg tcgcctacct gcgcgcctta cgcctgaatg ccgcacgccg ggagttgcta
    36121 aaaggcgcgt cacgggtctc ggtacaggac gtggccgcac gctggggctt ctggcaccta
    36181 agtcggttct ccagcgaata ccgggaaatg ttcggcgaac ggccctcaca aaccctgcac
    36241 cgctaggatc tattgccgtt tcgttcgcgg ccgtatcctg cgggtggggc cgcccaggtt
    36301 gcgcgaaaaa tcacgccagg cgatgttgca ggacgtggca agggaacgat gtggccgaac
    36361 tggcgcggat ggcgagtcca ttacctgtgt cctttgcctg cggaatgccg cttagcctgg
    36421 cgagattttg cgcggcaacg cggctcgtga cgaaacggca acagaccgcc ccgcaaaggg
    36481 gcatcgtacc cctctaccca accacctccc gggcgtactt ggtgcaccaa gcgctcgccc
    36541 attcttggcg gttacatctt ttctctcatc agcctgcatc tttgccgttt ttggctaaca
    36601 ggcccaacgc ccccctcgta acctgaatgt aacggacgaa ctgccacgtc ctggcctccg
    36661 cctatccagg cgggcaaaca caccctgttg acaataaaaa gtctcgcaac ggaggtaact
    36721 gggagatgga caagaaattc gccttgtatc ccctggccat gtcgatcatg gcctcgatgt
    36781 cccctgcagt tcaggcgttc caaatagaca gcgcagaccc cgatctcaga ctgcgctggg
    36841 acaacacgct caaatacagt accgccagcc gcctcaaagg cgcctcagac caactcacgg
    36901 cgcagcccaa tcatgacgat ggcgaccgta acttcagccg tggcgtgatc tccaaccgct
    36961 tcgacctgct atcggagttc gatgccacct acaaaaacat cggtgcgcga gtctccgggg
    37021 ccgcgtggta cgacagcgtc tataacgaag gcaatgacaa caactcccca gctacggcga
    37081 acgccttctc ggtcgacaac gatgaattca cccgcgccac cgaaaagctg catgggcgca
    37141 aggctgagct ccttgatgcg ttcctcttcg cgcgcttcaa tctgggcgat accaaaggca
    37201 ccgtgcgcgc cggtcgccac gccttggtat ggggggaaag tctgttcttc ggtgccaacg
    37261 gcattgcagg caccatggcg ccggtggacg cggtcaaagc ggcttcggta cccaataccc
    37321 agttcaagga gctaatccgc cccaccgaac agttctctgg ccagttgcag ctaacgcctg
    37381 atatatccat cggagccttc tatcagtggc gctgggacag aacacgtctg ccagcctcgg
    37441 gcagctactt ctcggtactg gatacgatca gcgatggtgc cgagcgcttg attgccgggc
    37501 ccaacccggt gattccgggc ggtgaaccac tggccttttt ccgtgaacag gacatgaagg
    37561 caaaagactc cggccagggc ggcgtgcaac tgcgctttag cgctggcgaa accgacttcg
    37621 gcctgtatgc cgttcgctac cacgacaaga cgccgcagat ttacattcgc ccagacgcag
    37681 gtgtcccaga cttcgccagc ggtcgcctgg gcacctacca gtgggtttat ccggaaaaca
    37741 tccgcgcatt tggtgccagc gccagccaca ccttcggcga ttacaacctg gcagcggaag
    37801 tctctacccg ccgcaacatg ccgttgagca gcgatggcgt gactatcgtc gacggcaccg
    37861 gcgacaacga tggtgatgcc cgttacgccg tcggtaacac cctgcatgca caggtgtcct
    37921 ggctggccac tctgggcccg tcgttcatcg ctaacgaagc agacttcgtc ggcgaagtgg
    37981 cttggaaccg cgtgctcagt gtgaccaaga accgccaggc gcttaaccct ctggcggacc
    38041 gcgacgcagc caacgtgcgt ttcgtctacg agcctcgcta tcgccaggta ttttcgggca
    38101 tcgacctttc cgtgccggtc ggtctcggct tcggtatggg caactccgca gtggtcggcg
    38161 ccttcaacgg cgacaaggtc ggggacgtca gcatcggtct aaccgcgctg tacctcaacg
    38221 aatggcgagc aggcctcacc tacacccact acttcggacc ggccggcacc tcggtcgacg
    38281 aagacaacca catctccttc aagcaatcgc tgaaagatcg cgacttcatt gccctgtcga
    38341 tccagcgcac tttctgagta gaggaaccct acatgacccg tttcttcctt cccgtattga
    38401 gcctgttgtt gctatgcgct cagcagagcc ttgcggccgt gaccgccgaa gaagccacca
    38461 agctgaaagg cgagctcacc ccgctcggag ccgaacgtgc cggcaacgcc gatggcagca
    38521 ttccagcctg gaccggcggg atgactacgc ccatccctgg cgacaagccg ggcggtcgcc
    38581 gttcggatcc gttccaaaac gaaaagccgc tgttcaccat taatgcgagc aacgccgccc
    38641 aatacgcgga caagcttacc gatggcaccc gcgcgatgct ggccaagtac cccgacagct
    38701 atcgcctcga cgtctaccag acgcaccgca ctgccgccgc gccgcaatgg gtctacgaca
    38761 acaccctgac taacgcgagc cgcgcccgca tcgataacga tgtagtggtc ggcgcattcg
    38821 gcggtatccc ctttccgatc ccgaccagcg gtgccgaggt gatgtggaac cacttgctac
    38881 gctggcgtgc accggctgca cgcttctacg ccacggccta ccagttgaca gccgatggcc
    38941 gcgccgtctt ggcgagcgaa gccagttctg tgcaacaggt accgtactac ttcaaggaca
    39001 ccaccctgga ggagtacgcc aagtccaacc tgtacttcaa catccgcgtc aacctgaccg
    39061 ccccggctat ccgcgctggc gagggcgtcg tcggcaggca gaacctcgac gcagccaagg
    39121 accaggcctg ggtctatctg gtcggccagc gtcgcgtgcg caagctgccg aatacgtgct
    39181 gcgatacgcc aagcccgacc atggcggggg tcatgagctt tgatgagctt gaggtgttct
    39241 ccggccggct ggatcgcttc gactggaaac tactcggcaa gcaggaaaag tacattccct
    39301 acaacgccaa caagctgctg cagccgacct ccgacaagga cgtactgagc gcccatcacc
    39361 tcaatcccga gcacgtgcgc tgggagctgc accgcgtctg ggtggtcgag gccacactgc
    39421 gtgacggcca gcgtcaccag gccgcacgca gcctgtatta ctgcgacgag gacacctgga
    39481 catgtgtgct gggcgaccgc tgggacgcaa atggtcagct ttggaaaact acctggggtc
    39541 aggtcatcgt gatgcccgat ctacctggca acttcatgtt cgccttcggc ttcaacgact
    39601 tgttgtccgg cgcagccatg gtcgccggtt tgttcaacga aaagccggtt cactacgagc
    39661 tgctcgaacg ccttccggat gtcgagttca cgcctgatgc cctggccaac gacggcatcc
    39721 gctgacatga cctgccatgg cagcacggag cccatcatga aaagaatgac gcaaacggca
    39781 gtagtcgatg caatcggatg gcgcggcgcg ctattggcat gctgccttgc cctggctccc
    39841 gtagcaggt
//
LOCUS       DASRUM010000300         8970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_986103, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000300 DASRUM010000000
VERSION     DASRUM010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8970
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986103"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..829)
                     /locus_tag="VFF22_20255"
     CDS             complement(95..829)
                     /locus_tag="VFF22_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZX19186.1"
                     /translation="MSQLSVIFKRELASYFATPLAYVFILIFLVLSGVFTFYLGGFFE
                     SGQANLSPFFNFHPWLYLFLVPAIAMRLWAEERKSGTIELLMTLPITRFDAVTGKFLA
                     AWVFAGLALLLTFPMIITVNYLGEPDNGAIVTGYIGSWLLAGAYLAIGSCMSALAKNQ
                     VIAFILAVSVCFLFIVSGFPMVLDGFSGWAPQWLVDAVASLSFLTRFDAISKGVIDLR
                     DLLYFLTLIAAWLAATAVVIDLKKAD"
     gene            complement(826..1749)
                     /locus_tag="VFF22_20260"
     CDS             complement(826..1749)
                     /locus_tag="VFF22_20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZX19187.1"
                     /translation="MIEIKNLTKRFAQHIAVDNLSFSVQPGEVLGFLGPNGAGKSTTM
                     KMLTGFLAPTSGSASILGFDIQKDTLKAQRQIGYLPEGAPCYGDMTVRSFLEFIAEVR
                     GFKGAEKRERVAKAVAQVELEAVLEQSIETLSKGFKRRVGLAQAILHDPKVLILDEPT
                     DGLDPNQKHQVRKLIQSLAYDKIVIISTHILEEVSAVCTRAVVIAHGKLLADGTPLEL
                     ESRSRYHQAVTLVAAEPLDQAALAALPGVAGVEENALEHSLSILAKPGEVIFPQVNAL
                     IAERGWQVKELNVERGRLDEVFRSLTRGEVV"
     gene            complement(1941..3110)
                     /locus_tag="VFF22_20265"
     CDS             complement(1941..3110)
                     /locus_tag="VFF22_20265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZX19188.1"
                     /translation="MDLNHYRMNWNYPTSVRVGVGRISELAASCRQLGMNAPLLVTDP
                     GLAALPMIDRALLHCRDDGLKAGLFHGIKGNPTGDNVMDGVAAFKAGKHDGVIAFGGG
                     SALDAGKAIALMAGQDRPLWDFEDIGDNCNRVNVAGMAPVVAVPTTAGTGSEVGRASV
                     ITDDAAHIKRIIFHARMLPALVILDPELTLGLPAKITAATGMDALSHNLEAFCSPLFH
                     PMAEGIALEGMRLVQQYLPRAVAQGSDVEARLQMLVASSMGATAFQRGLGAMHALAHP
                     LGALYDAHHGLLNAVLMPYVLVANRERIEPQMEKMARYLNLAKPGFSGVLEWVMALRE
                     QVGIPHSLGEIGIDEIRIEQVGQMAEVDPSAGTNPIAFTAAQYSQLFEKALRGTL"
     gene            complement(3119..4507)
                     /locus_tag="VFF22_20270"
     CDS             complement(3119..4507)
                     /locus_tag="VFF22_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZX19189.1"
                     /translation="MSKLQCISPIDGSVYVERHLASNPEIQAALVKAELAQQAWKNTP
                     LAERIAIGRKAIAAFAAKETQLAEELCWMMGRPIRYAAGEVRGFVERASYMADIAEQA
                     LADINVPEKPGFIRFIRREPLGVALVIAPWNYPYLTAVNAVMPALLAGNAVLLKHSAQ
                     TPLCAERMVEAFSEAGLPDGVFQYLHLSHGETEALIQAPTIAHVAFTGSVPGGTMVER
                     VAVGRFLSIGLELGGKDPAYIRADADLTHAVETTIDGAFFNSGQSCCGIERIYVHESL
                     YDAFVEQAVALVKQYKLGRSDDPDTTLGPLVRAEAADFVRGQIHDAIEQGATAHLVPA
                     DFALDHPGSQYLAPQVLTNVHHGMRVMTEESFGPVVGIQKVKDDEEALALMNDSEFGL
                     TAAIFSRDVDAAMALADRVQAGTVFLNRCDYLDPALAWTGVKNSGRGCTLSSVGFEHV
                     TRPKSFHFKTQL"
     gene            complement(4544..5917)
                     /locus_tag="VFF22_20275"
     CDS             complement(4544..5917)
                     /locus_tag="VFF22_20275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HZX19190.1"
                     /translation="MLNPRDVKTVLDAKRIVEERGLSHIKVGVFDNDGVMRGKYLSRS
                     KFFSALDSGFAFCDVVLGWDVKDQLYDNAQYTGWHTGYPDAPVRVLPHTCRDLPFENG
                     MLLFICEFAEAAEKVCPRGTLRRVIERCKAMGFDAFAALEYEFFMFDETPESAREKGF
                     RNLKPFTPDWFGYSMIRNSVHAELYHEILEMAERMDFPIEGLHTETGPGVLEAAIAYD
                     RAEAAADKGALFKTFMKVLAQRNGLMATFMAKWSGKYPGQSGHIHVSLRDLASGKSAF
                     YDPSQAHNMSKIQRHFLAGQQRLMPEFLCMVAPTLNSYRRLIPGFWAPTDATWGVENR
                     TAALRVIPGSDKSQRQEYRLGAADGNPFLALSVALGSGLYGVMQQWEPTEPVVGNAYA
                     MRHPEELALPRTLWDAAQRLKASTAARELFGDEFVEHFAASREWEEREYRRHVSDWEL
                     DRYFEII"
     gene            6277..7083
                     /locus_tag="VFF22_20280"
     CDS             6277..7083
                     /locus_tag="VFF22_20280"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX19191.1"
                     /translation="MKLIFRRTPAHGMTRHTTLATAGLTLSLMGLLAPTTGQARDWLI
                     YTHSLDSQAELIDGQLRGREHAGKRAFYLELVRALLADMAKPLPIQEVPLARGLAQLQ
                     QRDDVVLFNLGKTPERLALAHWVGPIWEETDWLYENTQRPTGIQNLLDAKNLSVCVLN
                     GSSHDERLSAMAFSQLKRNNTYSTCFSMLAAGRVALVASADSGLAQKLHEAQITTDSI
                     RPSAVQLGRDEGFIALSRGMPEQDIVRWRETLQRFKQDGRFQALRTRYAN"
     gene            complement(7153..7686)
                     /locus_tag="VFF22_20285"
     CDS             complement(7153..7686)
                     /locus_tag="VFF22_20285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HZX19192.1"
                     /translation="MTNLYFDNTQRYGRVTRMLHWAMAFLLGWQFLTTLVKVLMEESA
                     LDEFMWGTHKATGVLLMLMIVLRLSWALYNRTQRPASLGLAASLGHIALYGLMLCVPL
                     FALLRQYGSGRELTVFGMQLMPGFSGEKIEWLMLPANLFHGWGGWLLLLLIIGHALMV
                     FVHRSQGQDILPRMLGR"
     gene            complement(7859..8908)
                     /locus_tag="VFF22_20290"
     CDS             complement(7859..8908)
                     /locus_tag="VFF22_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZX19193.1"
                     /translation="MSTRRSFLKTAAVATGAAGATALGPAHIYAAEPKKIVWRLQTYA
                     GAALGEHVIKPSIDAFNKAANGEMEIQLYYADQLVPTGELFRAMQKGTIDAVQSDDDS
                     IAAPVDISVFGGYFPFATRYSLDIPVLFNQYGLNEIWAEAYGEVKGVTWLGAGAWDPC
                     HFATVEPITKLEQLKGKRIFTFPTAGKFLTRFGVIPVTLPWEDVEVAIQTGELDGIAW
                     SGITEDYTVGWANVTKYFLTNNISGAWCGSYFANSDKWAEVPEHLKTLFKLCMDSSNY
                     YRQHWYWGGEAQLRVEGGKLQLTSIPAEEWATVEAEALKFWDEIAKTSPRCARVVEIF
                     KKYNALMAKAGAPYR"
BASE COUNT         1843 a         2796 c         2691 g         1640 t
ORIGIN      
        1 aggctgacgc tggagaacag gttgaaggcg agaaacgcca gggcaatgag tgcgagcccg
       61 gcgctggaaa ccatcagctt tttcatgggc ggcctcagtc agctttcttc aggtcgataa
      121 ccaccgccgt cgcagccaac caggcggcga tcagggtgag gaaatacagc aggtcgcgta
      181 ggtcgatcac gcccttgctg atcgcatcga agcgggtcag gaagctcagc gaagccacgg
      241 catccaccag ccattgcggc gcccagccac tgaaaccgtc gagcaccatg gggaagccgc
      301 tgacgataaa cagaaagcac acgctgaccg ccaggataaa ggcgatgacc tggttcttcg
      361 ccagggccga catgcacgag ccaatcgcca ggtaggcgcc ggccagcagc cagctgccga
      421 tataaccggt gacaatcgcg ccgttgtccg gctcaccgag gtagttgacg gtgatgatca
      481 tcgggaaggt cagcagcagc gctaaaccgg caaacaccca ggcagcgagg aacttgccgg
      541 tgaccgcatc aaaacgggtg atcggcaggg tcatcagcag ctctatggtg ccgctcttgc
      601 gctcctcggc ccacaggcgc atggcaatcg ccggcaccag gaacagatac aaccaggggt
      661 ggaagttgaa gaacggtgac agattggcct ggccgctctc gaagaagcca ccgaggtaga
      721 aggtgaacac cccggacagc accagaaaga tcaggataaa tacgtaagcc agcggcgtgg
      781 cgaagtagct cgccagctcg cgcttgaaga tcaccgataa ctgactcata ccacctcccc
      841 acgggtcagg ctacggaaca cttcgtccag ccgaccgcgt tcgacattca actccttgac
      901 ctgccaaccg cgctcggcga tcagcgcatt gacctgcggg aagatcacct cacctggctt
      961 ggccagaatg ctcaggctat gttccagggc gttctcttcc accccggcaa cgcccggcaa
     1021 ggcagccagc gcggcctgat ccagcggctc tgcggccacc agggtgacgg cctggtgata
     1081 gcgcgaacgg ctttccagct ccagcggcgt gccatcggcc agcagcttgc cgtgggcgat
     1141 caccaccgct cgggtgcaga cggccgagac ttcttcaaga atgtgggtgg agatgatcac
     1201 gatcttgtcg taggccaggc tctggatcag cttgcgcacc tggtgcttct gattcggatc
     1261 gaggccgtcg gtgggctcgt cgagaatcag caccttgggg tcatgcagaa tcgcctgggc
     1321 caaaccgacg cgccgcttga agcctttcga cagggtctca atcgactgct caagcacggc
     1381 ctcaagctcg acctgggcca ccgccttggc tacccgctca cgcttctcgg cgcctttgaa
     1441 accgcgcacc tcggcaataa attcgaggaa gctgcgcacc gtcatatcgc cgtaacacgg
     1501 cgcgccttcg ggcagataac cgatctgccg ctgggccttg agggtgtctt tctggatatc
     1561 gaaaccaagg atgctggcgc tgccggacgt cggcgcaaga aagccggtaa gcatcttcat
     1621 ggtggtggat ttgccggcgc cattggggcc gagaaagccc agcacttcac cgggctgaac
     1681 gctaaaggac aggttgtcca ccgcgatgtg ttgcgcaaaa cgcttggtta ggttttttat
     1741 ttcgatcatg gcctctcacc attgcggtaa caggccctgc ttgcagccgg taatgacgca
     1801 agccgggcgc ttatgtgccg gcggactata aggagtcacg cgcggccagc gcaagcgaag
     1861 aaccggcggg tcatgtatct gttcgctaca gcgaggccag gcgcgagttg cttaacaacc
     1921 agcgcccggc agatcgcaga ctagagagtg ccgcgcaggg ctttctcgaa aagctgactg
     1981 tactgcgcag ccgtaaaggc aatcgggttg gtgccggcag aggggtcgac ctcagccatc
     2041 tgcccaacct gttcgatgcg tatttcatcg atgccaatct cgcccagact gtgcggtatg
     2101 ccgacctgtt cacgcagcgc catcacccac tccagtacgc cgctgaaacc gggtttggcc
     2161 aagttcaggt agcgcgccat tttctccatc tgcggctcga tgcgttcgcg gttggccacc
     2221 agcacatagg gcatcagcac cgcattgagc aggccatggt gcgcgtcgta cagcgcgccg
     2281 agcggatggg ccaaggcatg catggcgccg agaccacgct ggaatgcggt cgcgcccatg
     2341 ctcgaggcca ccaacatctg cagccgcgct tcaacatcgc tgccctgcgc caccgcgcgc
     2401 ggtaggtact gttgcaccag gcgcatgcct tccagggcga tgccctcggc catggggtgg
     2461 aacagcggtg agcagaaggc ctccaggtta tgcgacagcg catccatgcc ggtggcggcg
     2521 gtgatcttgg ccggcaggcc gagggtcagt tccggatcaa ggatcaccag tgccggcaac
     2581 atgcgcgcgt ggaagatgat gcgtttgata tgcgcggcat cgtcagtaat caccgaagcg
     2641 cggccgactt ccgagccggt accggcagta gtcggcaccg ccaccaccgg ggccatgccg
     2701 gcaacattga cgcggttgca gttgtcgccg atgtcctcga aatcccacag cggccgatcc
     2761 tggccagcca tcagggcgat ggccttgccg gcatccagcg ccgagccgcc gccaaaggcg
     2821 atcaccccgt catgcttacc ggccttgaat gcggccaccc cgtccatcac gttatccccg
     2881 gtagggttgc ccttgatgcc atggaacagc ccggccttga ggccatcatc acggcagtgc
     2941 agcagcgcgc ggtcgatcat cggcagtgcg gccaggccag ggtcagtgac cagcagcggc
     3001 gcgttcatcc ccaattgccg acaggaggcc gccagttcgc tgatgcggcc gacgccaacg
     3061 cgcaccgagg ttgggtagtt ccagttcatg cggtagtgat tcaggtccat ggcgcgcctc
     3121 acaactgagt cttgaaatga aaggatttcg gccgggtgac gtgctcgaag ccgacactgg
     3181 acagtgtgca gccgcgtcca gagttcttca ccccagtcca ggccagcgcc gggtcgaggt
     3241 aatcacagcg attgagaaac accgtgccgg cctgtacccg gtcagccagc gccatggcag
     3301 catccacgtc acggctgaag atggcggcag tcaggccgaa ctcgctgtcg ttcatcagcg
     3361 ccagggcttc ttcgtcatcc ttgaccttct ggatgcccac caccgggccg aaggattctt
     3421 cagtcatcac gcgcataccg tggtgcacat tggtcagcac ctgcggcgcc aggtactgcg
     3481 agccaggatg gtccagggca aagtcggccg gcaccagatg ggcggtggcg ccctgctcga
     3541 tggcatcgtg gatttgccca cgaacaaaat ccgccgcctc ggcgcgcacc aacgggccga
     3601 gagtggtatc cgggtcatcg gagcggccga gcttgtactg tttaaccagc gccaccgcct
     3661 gctcgacgaa ggcgtcatag agcgactcgt gcacatagat gcgctcgatg ccgcagcagg
     3721 attgcccgga gttgaagaac gccccatcaa tcgtggtttc caccgcatgg gtcagatcgg
     3781 catcagcccg tatataggct gggtccttac cgcctagctc caggcctatg ctgagaaagc
     3841 gccccaccgc cacgcgctca accatggtgc cgcccggcac tgaaccggtg aacgcgacat
     3901 gggcgatggt cggcgcctga atcagtgcct cggtttcccc gtggctcaag tgcaaatact
     3961 ggaacacccc gtccggcaag ccagcctcgc tgaaagcttc gaccatacgc tcggcacaca
     4021 gcggggtttg cgctgaatgc ttgagcagca ctgcattgcc ggccagcagc gccggcatca
     4081 ccgcattcac tgccgtcaga taggggtagt tccagggcgc gatcaccagc gccacgccca
     4141 gcggctcgcg gcggataaag cgaataaaac cgggcttttc cggcacattg atatccgcca
     4201 gagcctgttc ggcgatatcc gccatatagc tagcacgctc gacaaagccg cgtacttcgc
     4261 ctgcggcata gcggatcggc cggcccatca tccagcacag ctcttcagcc agctgggttt
     4321 ccttggcggc gaaggcagca atcgccttgc ggccgatggc aatgcgctcg gccagcgggg
     4381 tgttcttcca ggcctgctgc gccagttcgg ccttgaccag cgccgcttga atctcgggat
     4441 tggaagccag atggcgctcg acatataccg agccgtcgat gggtgaaatg cattgcagtt
     4501 tgctcattga aaaactccat aagcccaaac agccggggcc gcgctagata atttcgaagt
     4561 agcgatccag ttcccagtcg ctgacatgcc ggcgatattc gcgctcttcc cactcgcggc
     4621 tggcggcgaa gtgctcgaca aactcatcgc caaacagctc ccgcgccgcc gtcgaggctt
     4681 tcaggcgctg ggccgcatcc cacagggtgc gcggcagggc cagctcttcc ggatgcctca
     4741 tggcgtaagc attgcccacc accggctcgg ttggctccca ctgctgcatc acgccgtaca
     4801 ggccagagcc gagcgccacc gacagggcta gaaatggatt gccgtcggcc gcacccaggc
     4861 gatattcctg gcgttgggat ttgtcgctgc cggggattac ccgcagcgcg gcggtgcggt
     4921 tttccacgcc ccaggtggca tcggtgggcg cccagaaacc ggggatcaac cgccggtagc
     4981 tgttcagcgt tggcgcgacc atgcagagga attccggcat cagacgttgc tgtccggcga
     5041 gaaagtggcg ctgaatcttg ctcatattgt gcgcctggct cgggtcatag aacgccgact
     5101 tgccgctggc cagatcacgc aacgacacat gaatatgccc gctctgcccc gggtacttgc
     5161 ccgaccactt ggccataaag gtggccatca ggccattgcg ctgggccagc accttcataa
     5221 aggtcttgaa cagcgcgccc ttgtcggccg ccgcttcggc gcggtcatag gcaatcgccg
     5281 cctccagcac gccagggccg gtttcggtgt gcaggccttc gatgggaaag tccatgcgct
     5341 cggccatttc gagaatctcg tggtacagct cggcgtgtac cgagttgcgg atcatcgagt
     5401 aaccaaacca gtccggggta aagggtttga gattacgaaa acccttctcg cgggccgact
     5461 ccggggtttc atcgaacatg aagaattcat attccagcgc agcaaaggca tcaaagccca
     5521 tggccttgca gcgctcaatc acccggcgca gggtgccgcg cggacagacc ttctccgccg
     5581 cctcggcaaa ctcgcagata aacagcagca tgccgttttc aaagggtaag tcgcggcagg
     5641 tatgcggcaa cacccgcacc ggcgcatccg ggtagccggt gtgccagccg gtgtattggg
     5701 cgttgtcgta gagctggtcc ttcacgtccc agccgagcac cacatcgcag aaggcaaagc
     5761 cgctgtcgag ggcagaaaag aacttgctgc ggctgaggta cttgccgcgc atcaccccat
     5821 cgttatcaaa taccccaacc ttgatatggc taaggccgcg ctcctcgaca atgcgcttgg
     5881 catccagaac ggttttcacg tctcgcggat tgagcataaa tgcacctgtc tcttgttgtt
     5941 atgactgtcg ttatggccga catccggggc aacaacagag tctcaagcgc atccccacaa
     6001 caaccgcaca acgcgccctg actaccaccc aacgcctgtt agaacacccg gaaaaagccc
     6061 agttagtgtg gtacaggatt gccgagttgg ctgagcgatt gcacaaaaca acctgttgga
     6121 ggcgcggcca gtggtggatt gcgctttaca gctgagccgc acagtcatta gatggcggtg
     6181 ccatgtggtt cacgataagg cgccaggccc gcgccttggc taaagtctgg ctgcagaagc
     6241 ggataaaact gctcaagggt tcatacagga ggttgtttga agttgatttt ccgccgtacg
     6301 ccagcgcacg gcatgacgcg ccatacaacc cttgccacgg cagggctgac gctaagcctg
     6361 atgggcttgc tcgcccccac gaccggtcag gcgcgtgact ggctgattta tacccatagc
     6421 ctggacagcc aggccgagct tatcgatggg caattgcgcg ggcgcgaaca tgccggcaag
     6481 cgagcgtttt atctggagct ggtgcgcgcc ctgttagccg acatggccaa gccactgccg
     6541 atccaggagg tccccctggc gcgtggcctg gcacaactcc agcagcgcga cgatgtggtg
     6601 ctcttcaacc tcggcaaaac gccggagcgc ctggcactgg cacattgggt ggggccgatc
     6661 tgggaggaaa ccgactggct ttacgaaaac actcagcgtc ccaccggcat tcaaaacctg
     6721 ctggatgcca agaacctctc ggtgtgcgtg ctgaacggca gctcccatga cgaacgtcta
     6781 tcggccatgg cgttctcgca gttaaaacgc aataacacct acagcacctg cttcagcatg
     6841 cttgcggccg gccgggtcgc gctggttgcg tcggccgact cagggcttgc gcagaagctg
     6901 cacgaagccc aaatcaccac tgacagcatc cgcccgagtg ctgtgcaact cggccgcgac
     6961 gaaggcttta tcgccttgtc ccgcgggatg ccggagcagg atatcgtgcg ctggcgcgaa
     7021 accctgcaaa ggtttaagca ggatgggcgt ttccaggccc tgcgcactcg ctacgcaaac
     7081 tagcctcctt gatcagcctg cccaggctgg tgcgacgaca tcacggcagc aatccaaaca
     7141 cctgagtcgg gattatcgac ccagcatacg cggcagaatg tcctggccct ggctgcggtg
     7201 aacaaacacc atcagcgcat ggccgattat cagcagcaac agcaaccagc cgccccagcc
     7261 atgaaacaga ttggctggca gcatcagcca ttcgatcttc tcgccgctaa agcccggcat
     7321 cagttgcatg ccgaacaccg tcagttcacg tcccgagccg tattggcgca gcagggcaaa
     7381 cagcggcaca cagagcatca agccatataa cgcaatatgc cccaggctcg ccgccagccc
     7441 cagcgatgcc ggacgttgcg tgcggttgta cagtgcccag ctcaagcgca gcacaatcat
     7501 cagcatcagc agcacaccgg tggccttgtg cgtaccccac ataaattcgt ccagagcaga
     7561 ctcctccatc agcaccttca ccagggtggt gaggaactgc cagcccagta aaaacgccat
     7621 ggcccagtgc agcatgcggg taacccggcc gtagcgctgg gtgttatcga agtaaaggtt
     7681 tgtcatggtg cgtgtctcga aacagatagg gtcgggcgca agtgtaaagc ctgtggtgtg
     7741 ctcatcgcgg caaagagtat tgcagcgatt aagtgcagcc ccgcaggcaa cgccaaggcg
     7801 aaagcaaaac gcccgctcct tgcggcgcgg gcgtgactct gtaggcggag cgacgctatc
     7861 aacgatacgg cgcgccggcc ttggccatga gggcgttgta ctttttgaag atttccacca
     7921 cacgggcgca gcgggggctg gttttggcga tttcgtccca gaacttcagc gcctcagctt
     7981 ccaccgtggc ccactcctcg gccgggatag aggtcagttg cagcttgccg ccctcgaccc
     8041 gcagttgcgc ttcaccgccc cagtaccagt gctggcggta gtagttggag ctgtccatgc
     8101 acagtttgaa cagggttttc aggtgctcgg gtacctcggc ccatttgtcc gagttggcga
     8161 agtaggaacc gcaccaggcg ccggagatgt tgttggtaag gaagtacttg gtcacgttgg
     8221 cccagccgac ggtgtagtcc tcggtgatgc cggaccaggc aatgccatcc agctcgccgg
     8281 tctggatcgc cacttcgacg tcctcccagg gcagggtcac cggaatcacg ccaaagcggg
     8341 tgaggaactt accggctgta gggaaggtga agatgcgctt gcctttgagc tgctcaagct
     8401 tggtgatcgg ctcgaccgtg gcgaagtggc aagggtccca ggcgccggca ccgagccagg
     8461 tcacgccttt gacttcgcca taggcctcgg cccagatttc attgaggccg tactggttga
     8521 acagcaccgg aatatccagg ctatagcggg tggcaaaggg gaagtagccg ccgaacaccg
     8581 agatatccac gggcgcggcg atggagtcgt catcgctctg caccgcatcg atggtgccct
     8641 tctgcatagc gcggaatagc tcgccggtgg gcaccagttg gtcggcgtaa tacagctgga
     8701 tttccatctc accattggcg gccttgttga aggcatcgat ggacggtttg atcacatgct
     8761 cgcccaatgc cgcaccagca taggtctgca aacgccagac gatttttttc ggctcggcgg
     8821 cgtagatatg cgccgggccc agtgccgttg cccctgctgc cccagtggcc accgcggcgg
     8881 ttttcagaaa actgcgtctc gtactcatgc tgttatctcc aggcttgtcg attcagatgg
     8941 catacaaccg cactgcctgg cccacggggc
//
LOCUS       DASRUM010000301         4044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2324510, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000301 DASRUM010000000
VERSION     DASRUM010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2324510"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..340
                     /locus_tag="VFF22_20295"
     CDS             53..340
                     /locus_tag="VFF22_20295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4642 family protein"
                     /protein_id="HZX19194.1"
                     /translation="MRKDKKQVIGEEIGDEPIKLFLSVEPADATPPPLHKLIKAYRGL
                     RIDDFERFVGFFVAAGYDLNAKDAQGNDFIALIQDQRFAESYIEVIKAARG"
     gene            507..1100
                     /locus_tag="VFF22_20300"
     CDS             507..1100
                     /locus_tag="VFF22_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017519097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZX19195.1"
                     /translation="MNSSIRLDKRDLILAKGAEVMTRRGYHGAGVQEIVQAAGVPKGS
                     FYHYFASKEDFALQALQQVYQPRLARYAEALANPALSPRERILGYYAELVEHFARQER
                     LEYHCFIGSLSFEMAELSPTLGAQVDAILQASADTLQACLEQAQAAGELPADEDCRNL
                     ASFIANAWQGALTRLKVANNTQALNDFIQRLQLLLQA"
     gene            1158..2267
                     /locus_tag="VFF22_20305"
     CDS             1158..2267
                     /locus_tag="VFF22_20305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HZX19196.1"
                     /translation="MTDPIKALFQPFTLGNLELPTRVVMAPMTRSFSPGGVPNSKVIE
                     YYRRRAAGGVGLIVTEGTTVGHKAANGYPNVPRFYGEDALAGWKNVVEAVHAEGGKIV
                     PQLWHVGNVRKLGTEPDASVPGYGPSEKIKEGNVVVHGMTQDDINEVIAAFAQAAKDA
                     KAIGMDGVEIHGAHGYLVDQFFWAGSNQRTDEYGGDLSQRSRFAIELIQAVRAAVGPE
                     FPIIFRFSQWKQQDYSARLVETPEALEAFLKPLSDAGVDIFHCSTRRFWEPEFDGSEL
                     NLAGWTRKLTGKPTITVGSVGLDGEFLQFMVNTDKVAQPASLENLLERLNKQEFDLVA
                     VGRALLVDPDWAVKVRDGREQDILPFSRDALTSLV"
     gene            2500..3447
                     /locus_tag="VFF22_20310"
     CDS             2500..3447
                     /locus_tag="VFF22_20310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZX19197.1"
                     /translation="MKRVVQISVSLVLLLLISLVAAVAWNWAPDRSLADLKPRWALPP
                     SEFLAIDGMLVHLRDQGPRDDPAPIVLLHGTSASLHTWEGWVVALQTKRRVISLDLPG
                     FGLTGPFPDGDYRMAHYSAFLAHVLDQLQVPRAVVAGNSFGGQLAWQFALDQPQRVER
                     LVLVDAAGYPRNATSIPIGFRLAQIPALAPLMANVLPRQMIEASIRNVYGDPSKVSDE
                     LIDRYYELTLRAGNREALRQRFIQAEAGQSYTRIAELKVPTLIIWGGRDELIPPANAE
                     RFKRDIKGSRLVLFDELGHVPQEEDPARTVAVLMGFLEG"
     gene            3614..>4044
                     /locus_tag="VFF22_20315"
     CDS             3614..>4044
                     /locus_tag="VFF22_20315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004421745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HZX19198.1"
                     /translation="MPKAIRLFVHAVEALNRVVGRFAMYLIFAILGVLLYSSISKTFF
                     MPAAWTLESAQFLMVAYFLLGGAYSMQLDAHVRMDLFYSRWSPRARAIIDAITIGFLI
                     FYLVFLLYGGISSTQYALEYSETSYSAWSPPMAPIKIIMCIG"
BASE COUNT          763 a         1219 c         1210 g          852 t
ORIGIN      
        1 gccccggagg tacactgccg cccccgtgtg gcctggttag ctggagtgag tgatgcgtaa
       61 agacaagaaa caggtgattg gcgaagagat tggtgacgag ccgatcaagc tgttcctaag
      121 cgtggaaccg gccgatgcca cgccgccgcc gttgcacaag ctgatcaagg cctaccgtgg
      181 cctgcgtatc gatgatttcg agcgctttgt cggtttcttc gttgctgctg gctacgacct
      241 gaatgccaag gatgcccagg gcaatgactt tatcgcgctg atccaggatc aacgctttgc
      301 cgaatcctat attgaagtga tcaaggctgc tcgcggctaa taagccaagt cagcgcttgc
      361 agcgctgatt aaggctcacg cctgcccgaa aagccgcgac ctgttcgcgg ctttttcgtt
      421 tctaaaaccc gttctgcttc ttactgataa tcacgcaaat taatagtgct gggcggggcg
      481 agacgaccgg tcatattctt gccggtatga actccagcat ccgactcgac aagcgtgacc
      541 tgatcctcgc caaaggcgcc gaggtgatga cccgtcgtgg ttatcacggc gccggtgtgc
      601 aggaaatcgt ccaggcggcg ggggtgccga aaggctcctt ctaccactac ttcgccagca
      661 aggaagactt tgccctgcag gccctgcagc aggtctatca gccgcgcctg gcgcgttatg
      721 ccgaagccct ggccaatcct gcattaagcc cgcgcgagcg catcctcggt tattacgccg
      781 agctggtgga gcacttcgct cgccaggagc gtctggaata ccactgcttt attggcagcc
      841 tgagcttcga gatggccgag ctgtcaccga ctctgggcgc gcaggtcgat gcgattttgc
      901 aagcctcggc ggacaccctg caggcgtgtc tggaacaggc gcaggccgct ggtgaactgc
      961 ctgctgatga ggattgccgc aacctggcga gctttatcgc caacgcctgg cagggtgcgt
     1021 tgacccgctt gaaagtagcg aacaacaccc aggcacttaa cgactttatc cagcgtttgc
     1081 agctgttgtt gcaggcctga ttttttaaaa caccttgatc aagacgaccg gtcgtgtggc
     1141 cggctagagg aacgatgatg accgatccca ttaaagcgct gttccaaccc tttaccctgg
     1201 gcaacctgga gctgccgacc cgtgtggtca tggcgccgat gacccgttcc ttctccccgg
     1261 gtggcgtacc caacagcaag gtcatcgagt actaccgtcg ccgcgccgct ggcggtgtgg
     1321 gcttgatcgt caccgaaggc accaccgtcg gccataaagc cgccaatggt tacccgaacg
     1381 tgccacgttt ctacggtgaa gacgccctgg ctggttggaa gaacgtggtc gaggcggtgc
     1441 acgccgaagg cggcaagatc gttccgcagc tgtggcacgt gggcaatgta cgcaagctgg
     1501 gcaccgagcc ggacgccagc gtcccaggct atggcccgag tgagaaaatc aaagaaggca
     1561 acgtggttgt gcacggcatg acccaggacg acatcaacga agtgatcgcc gccttcgccc
     1621 aagccgctaa agatgccaaa gccatcggca tggacggcgt ggaaatccac ggtgcccacg
     1681 gctatctggt cgaccagttc ttctgggccg gcagcaacca gcgcaccgac gaatacggtg
     1741 gtgacctgtc gcagcgttcg cgctttgcca tcgaactgat ccaggccgtg cgcgccgcag
     1801 tcggcccgga gttcccgatc atcttccgct tctcgcagtg gaagcagcag gactacagcg
     1861 cccgtctggt ggaaaccccg gaagccctgg aagccttcct caagccgctg agcgacgcag
     1921 gcgtggatat cttccactgc tcgactcgtc gcttctggga gcctgaattc gacggctccg
     1981 agctgaacct ggctggctgg actcgcaaac tcaccggcaa gccaaccatc accgtcggca
     2041 gcgtcggcct ggacggcgag ttcctgcagt ttatggtcaa caccgacaag gtggcgcagc
     2101 cagccagcct ggaaaacctg ctggagcgcc tgaacaagca ggaattcgac ctggttgccg
     2161 taggccgcgc gctgctggtc gatccggatt gggcggtaaa agtacgcgat ggccgcgagc
     2221 aggacattct gccgtttagt cgtgatgcgc tgaccagcct ggtctaaata tccgttttat
     2281 cgtgtgcgcc gcaaaacggc gcaacactcc tcaagccccg gcacatcggg gctttttttc
     2341 gtctgcatat tgagggagcg gggggacgcc tagttccatg cccgagaagc ccttatcgcg
     2401 gccatgggcc gctcctacaa ccgggtggtg cacgacggtc acaggcaagc tgcgcctcgt
     2461 tgactagtat tgccagtgcc ccgccatcag gtaccgacta tgaaacgtgt tgtgcagata
     2521 tccgtatccc ttgttctgct gttgctgatc agcctggtcg ctgcggtcgc ctggaactgg
     2581 gcgccggatc gttctctggc ggaccttaag ccgcgctggg ctctaccacc gtcagagttt
     2641 ctcgccatcg acggcatgct ggtgcacctg cgtgaccagg gccctcggga tgacccggcg
     2701 ccgattgtgc tgctgcacgg tacctcggcc agcctgcaca cctgggaggg ctgggtggtg
     2761 gcgctgcaga ctaaacgtcg agtgatcagc ctagatctgc cgggctttgg cctgaccggg
     2821 ccattccccg atggcgatta ccgcatggct cactacagcg cctttctcgc ccatgtactg
     2881 gatcagttgc aggtgccgcg tgcggtggtg gcgggcaaca gttttggcgg gcaactggcc
     2941 tggcaatttg ccctggacca gccgcagcga gtcgagcgtc tggtgttggt agacgccgcc
     3001 ggttatccgc gcaacgcaac ctccattccc atcggttttc gcctggcgca gatccctgcg
     3061 ttggcgccgc tgatggccaa cgtgctgcca cgacaaatga tcgaggcgag cattcgcaac
     3121 gtctatggcg acccgagcaa ggtcagcgat gaactgattg atcgctacta cgagctgacc
     3181 ctgcgcgcgg gtaatcgcga agccttgcgc cagcgtttta tccaggccga agcggggcag
     3241 agctacacac gcattgccga gctcaaggtg ccgacgctga ttatctgggg cggccgcgat
     3301 gaattgattc caccggccaa cgccgagcgc ttcaagcgcg atatcaaagg cagccgtctg
     3361 gtgctgttcg acgagctggg ccatgtgccc caagaagagg accccgcgcg caccgtggcg
     3421 gtgctgatgg gctttctaga gggttagccg gggtttaggg cggctttttg ctgcgcacta
     3481 agctgctgat atttcgagag agattgcctg cgcaaaagag ctggcgtgct ttgtgctaac
     3541 ccactttgcg ctgattggct ttggccaatc ttggtctagg ctctcggaat aacaattatt
     3601 cggcaggtgc agtatgccca aggccatcag gctgtttgtg cacgcagtgg aggcgctcaa
     3661 ccgcgttgtc gggcgctttg ccatgtacct gatcttcgcc attctcggcg tgctgctgta
     3721 ctcctccatc agcaagacct tctttatgcc ggccgcctgg acgctcgagt cggcgcagtt
     3781 tttgatggtc gcctacttcc tgcttggcgg cgcctattcc atgcagcttg atgcccatgt
     3841 gcgcatggac ctgttctaca gccgctggtc gccacgggcc cgcgcgatca tcgatgcgat
     3901 caccatcggc tttctgattt tctaccttgt gttcctgctt tacggcggta tttcctcgac
     3961 ccaatacgcc cttgaataca gcgaaaccag ctactcggcc tggtcgcccc ccatggcgcc
     4021 gatcaagatc atcatgtgca tcgg
//
LOCUS       DASRUM010000302        19125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_331588, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000302 DASRUM010000000
VERSION     DASRUM010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 19125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19125
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331588"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2479)
                     /locus_tag="VFF22_20320"
     CDS             complement(<1..2479)
                     /locus_tag="VFF22_20320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HZX19199.1"
                     /translation="MSALTGFRISPQQARAWRDTQQQARANAASWLLIEPAQAPTPAE
                     IEARLQGLGEREEILRTALHAVPGMALPIQVIQDVARVTFVVEDWRGLCAQEHGARLT
                     RYREEQVDESVLSVCYARTADERWTLWLKGSAALLDQTSLELIAAELLGAPADERLQY
                     VDYAEWKNSLLEEEAEGQAVRFWQQARTESAPLELHGLQTSAASALYQPALQPLHNVP
                     TRRELELAAEQADMSLEEWLFSAWCVLLARLSGQPRIQLGWCDAGRGEGLEQALGLFE
                     QVLPIQAEVDLHASLKAQASTLLGQLRQARGWRDYYDSEQVCEVFFTCRDAASLQSHG
                     ELLDAGSLRQRFALGLECRLDAATLELRLAYDRTRFDTTAIACIEEQWTLLLRGLCAP
                     AQPIGALSLVGPRQDELLGNAAQAQVRAIGVLGLIEERARATPNAPALADAGGITHYG
                     ELLDQVESLARRLCAAGVQTGDVVGILLRRERQAIVAMLAALKAGAAYLPLDPSYPAE
                     RLAYMIENSGARLLLSTREQQGSVASSARTLLLDVTHGELPESVSLPAVAPTQTAYLI
                     YTSGSSGQPKGVPISHASLSHSTQVRMAYYPEPVQSYLLLSSLSFDSSVAGIYWTLCQ
                     GGLLVLPASGEELDLEALAGLIERHRISHSLSLPSLYETLLDYCPAPALAHLRTWIVA
                     GEPCTARVLEKHRSKAPQAQLVNEYGPTEATVWATAQVLSNTAPGDASISIGKPIPGM
                     QLWLLNDSAQQAAIGEPGEIHLGGPTLASGYLGLPEQTASAFVRYPHIGDGQRLYRTG
                     DLARRRADGQLDFLGRRDQQIKIRGHRV"
     gene            complement(2517..3569)
                     /locus_tag="VFF22_20325"
     CDS             complement(2517..3569)
                     /locus_tag="VFF22_20325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015372156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HZX19200.1"
                     /translation="MISTPPSGNRPSTGIARRKPIVLSEQSIVSERLLDPAWDLPWLV
                     EPNLPGVDLAQWAAQNRDRLEQKLLQHGAILLRGFDVQAAAHFNAVIDALSSGALEYM
                     FRASPRTRVGGNIYTSTDYPADQVIFPHNEHSYSPRFPLRLFFYCHQPSEIGGETPIG
                     SVRAVKAQIPPEIIETFRQKKILYVRNYGDGFGLPWQSVFQTDDRAEVETYCASVGIE
                     VEWKDGNRLRTRQVGSALARHPRTGEEVWFNHGTFFHLSTLPAAIRDSLDAGFSPMDL
                     PTNTFYGDGSPIESDVLETLRAAYLGSLVKFRWQRGDILLLDNMLAVHGREPYSGPRK
                     IYTGMAEATAGNDVQL"
     gene            complement(3585..5039)
                     /locus_tag="VFF22_20330"
     CDS             complement(3585..5039)
                     /locus_tag="VFF22_20330"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HZX19201.1"
                     /translation="MVKPCPPLMPLSESAARCVRQSFAQSRLWFLHQMDPGRAQHNIL
                     VRLVLNGPQLVETRLKHAFNALCIRHPLLRACYRMTPAGPEQWLAAGQSIEVKQRDLS
                     NLPAAEQKQAIDRLMHDEQNRPFDLESGPVIRACLLDLGLNVQELIIVIHHIAFDGRS
                     GEILLQDLAHYYATQEPGALLPGRLQYADFAVWEPGYVTAELIEVETAFWRDHLAGMP
                     LQLDFSKRPRPHGDTQGGRHQFVIQPLQVAHLKRASRAQRLPLFVMLTGLFQLWLHYL
                     SGQRRFLIGTDVHGRDLPELQDIFGFFVNQLTLKCELEDGLPLGELLMRTRHAIRLAH
                     AHRQLPFDLLVSQLAPQREAGRSPLFQTKFNYQRDRFSIDTLGDARIVRTQVIQDLAG
                     FDLVLDLMHRHDSIEVTLEYDRRLFSPDEIERFAHLWQRLLGALDTLLELPISMLRER
                     LQNWDDAHLQQQQHTLNNQGRARLGNTQRRAVSL"
     gene            complement(5048..6046)
                     /locus_tag="VFF22_20335"
     CDS             complement(5048..6046)
                     /locus_tag="VFF22_20335"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017520931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HZX19202.1"
                     /translation="MSQLADVCATDAIRSPLEQDDCVIAPLLHSHALPLLINSQQRDS
                     IHAMSDEIRAIALDALGTTGGVLFRNFKVPTPLDFKRFAASFGMPLGTYEFGSTPRSK
                     VFAGVYSSTEYPAHQSIPLHNEQSYTRQWPSRIWFHCMTASQSGGETPIADSRQVYRE
                     IDPTIREEFIAKGLLYVRNYSSALDIPWQQVFNTDDRGKVEQYCRAQGIEWSWNADGE
                     LSTKQLCQAAVQHPVSHEWVWFNQAHLFHISAMSADLRQTLIEAVGPAQLPRNVYFGD
                     GTPIPDATLDAIRAVYARASVTFAWQAGDILMLDNLLVAHGRNPFSGDRKVIVAMA"
     gene            6431..8512
                     /locus_tag="VFF22_20340"
     CDS             6431..8512
                     /locus_tag="VFF22_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZX19203.1"
                     /translation="MNKFKKNTLALAVTSGLMVATSALAAEVAEKEQPLQLKEVVVTG
                     EKIQRTQDKTLSSVAVVTSEDIRTHGDQDLQSVLDRTPGVYAQSGNENWGIRGIPVSG
                     FDDQGPAAMNGAVSVYVDDALQVNRLLTLNPLPLWDMEQVEVYRGAQSTTQGRNSLAG
                     AIIMRTKNPTFDPEFSVRGNVGKYGEQGTSFVANGALVEGVIAARLAVDYQTADGHIE
                     NETLDMDANQLRSANIRSKFLIQPSDDLDVLLTFARTKQRSGVNAVAANNGSPDYFSL
                     YENTETHNTLEQDTATAKVDYRLDDFWTLTSVTSGTWSEYHSILDFDRLPTATQTAPR
                     HHEQTLGSQELRLGYASEQVNGVIGAYLGRFKGEIDDQLVNNGMVLLDQQGETKINSQ
                     ALFGELNWEFVEDWQLITGLRWDHEKNNTKIEYPTDVLNIATDPSADEDISSSVLLPK
                     VGLSYDLTDNHVLGLTWQRGYRSGGVNVRTRARHATYDPEFTSTYELAWRGNWLDRRL
                     RTSANLYRTTWKDQQVSFLNLSDNTVQVANAAESRMVGLELTAEYLVTSQLLLNAGLA
                     YNDTKYESFVRDGVDLSDREFVFAPKKMANIGANYTFGNGLMIGGDVVYQGKSISNFN
                     TSGSTVTGERYNDSVALVNLNAEYPLGDLTLSGYVRNLFDERYITNNQSDSLLDVGAP
                     LTVGVAARYDF"
     gene            8641..10053
                     /locus_tag="VFF22_20345"
     CDS             8641..10053
                     /locus_tag="VFF22_20345"
                     /EC_number="2.6.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008006578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HZX19204.1"
                     /translation="MSVFSPIMSATADLQRRHSGLRERYCLDTTPLLQRQMERESNAR
                     SYPRRIPLELKRAQGIYVEDSRGQVYIDCLAGAGTLALGHNHPVTVEAIQRALSSGVP
                     LHTLDLSTPIKDAFVQTLFDLLPSDFAKNARIQFCGPSGADAVEAAIKLSRTALGQQT
                     ILSFHGAYHGMTLGTLSLSGNLGPKKSLGGLLAGVQFLPYPYSYRCPFGLSGEQSIDA
                     GLHYIRHLLSDPESGITQPAAMILETVQGEGGVIPAPERWLQGLRNITQEHGITLILD
                     EIQCGIGRTGDLFAFEQSGITPDVITLSKAIGGGLPLSVMVYRNELDQWQPGAHAGTF
                     RGNQLAMAAGDATLRFIQQEQLTTHVRCVGNQLQLLLQGLQAEFDWVGDVRGRGLMLG
                     MEIVDPESSDSLGRPAADGDKARKLQRACLERGLIVELGGRHGATVRFLPPLIISEME
                     IEHVGQILYQAAQAVHAVFS"
     gene            complement(10174..11364)
                     /locus_tag="VFF22_20350"
     CDS             complement(10174..11364)
                     /locus_tag="VFF22_20350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007248604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX19205.1"
                     /translation="MSAIESPRSLICTSASSTLSITILAISAFLIVTTEFLIIGLLPA
                     LARDLGISIAAAGQLVTLFAFTVMLFGPPLTAALAHLDRKKLFIAILLVFAFANGLAA
                     LSSNIWVLAIARFIPALMLPVFWGTASETAAQLAGPAKSGQAIARVYLGITAALLLGI
                     PLGTLAANGIGWRGAFWLLAGLSLLMAVAMAIYMPKVARSARVDFLKQARIFKEPPFL
                     ANVALSIIIFSAMFIAYTYLADMLERIAGVDPAHVGWWMMGFGAIGLVGNWIGGKAVD
                     RSPLKATALFVVLLAAGMAAVTPLASAGLLICIALGLWGIANTALYPICQVRVMNSVS
                     HSQALAGTTNVSAANAGIGIGAIIGGLTIQTAGIASVGYVAAAVAGLALLIIPLMSRL
                     ATPK"
     gene            complement(11454..12395)
                     /locus_tag="VFF22_20355"
     CDS             complement(11454..12395)
                     /locus_tag="VFF22_20355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019485112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX19206.1"
                     /translation="MFSSERLKGIDVFVCVAELGSFAAAAERLSLTSSAVSKGIARLE
                     TRLGIRLFERTTRKLALTDAGTAFYRTCTRVLADLEESEMALHAEFFEPRGRIRIDLP
                     ASYGRMHVLPVILQLAKQHPLLMPHISFTDSFVDPADQGIDILVRIGGSDIWPATLSH
                     HYLGAERLIYCAAPEYLSRRGTPASEAELEQHDCVLYGHSDGQVSPWYREGPAPGDKK
                     RRIMPARIAVGDGEGEVLAVLAGYGIAQLPTWLVQGHLDSGALVEVLPQLATDGLPMN
                     LVWLKKRETLPKVSVLLEALAICLTPAGHNLPEQADK"
     gene            complement(12529..12837)
                     /locus_tag="VFF22_20360"
     CDS             complement(12529..12837)
                     /locus_tag="VFF22_20360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003203770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HZX19207.1"
                     /translation="MSIATTGFGTLIIVLIMAAVTLATRWGGVFVMSYVPISYRVQQF
                     ISAMSGSVLVAILTPLAIDGDMSARLALLTTAIVMLLVKKPLPAIAAGILVAAISRQF
                     "
     gene            complement(12834..13544)
                     /locus_tag="VFF22_20365"
     CDS             complement(12834..13544)
                     /locus_tag="VFF22_20365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HZX19208.1"
                     /translation="MSDSATQLHEFSRQDVWAGFKQLVPLSIFVIAFGAAFGLAAVQT
                     GLDNPSAVLMSTLVFAGASQFAALELWGVQIPLVPLALTVFAINARHLLMGASLYPWL
                     RHLSPAKRYGVMLVASDANWALSIQAFNRGKPGFGLLLGGGLALWSFWIFGTWLGIYF
                     GGAIHDPKSFGLDMVMGCFLLAMAVGGEKNLRLLLIWTVAAGSSLLAYWYLPDNSHVV
                     VGALAGGVLGALWMEKKK"
     gene            13721..14584
                     /locus_tag="VFF22_20370"
     CDS             13721..14584
                     /locus_tag="VFF22_20370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZX19209.1"
                     /translation="MNVQDLQIDWLKCFVAVVDAGSLSGAAPEVNRSQSAVSMQLKKL
                     EAALGCQLLMRGPRHHQLTSEGQLLLGYARRMLDLHAETQAAFLGEELTGRIRLGVPD
                     DYAARYLTPVLKRFAPQHGAVEIELACEQSTSLIPRVERGDLDLALVSRDQLRHGTLL
                     FHEPMVWVGSAQFQVWRRDPLPIAVYEDSSLAKRSAINSLALQGRRYKVVYNSSSLAG
                     QIAAVESGLAVAVLTQCSAPTHLEILGSDHGLGPLEPMEVAVYRSRVSQGSKAVDSLH
                     SLLIRTLRLST"
     gene            14728..14979
                     /locus_tag="VFF22_20375"
     CDS             14728..14979
                     /locus_tag="VFF22_20375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010207618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZX19210.1"
                     /translation="MDYLLLTQRLGQQIRERRLNRGLTQAGLAEIAGLTRQKIIAVEK
                     GSISVGMTAYARVLGALGCELSVIPASMPTLEEVQGVFE"
     gene            14976..16172
                     /locus_tag="VFF22_20380"
     CDS             14976..16172
                     /locus_tag="VFF22_20380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020307383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HZX19211.1"
                     /translation="MSAPLQVSTPAGDSGRIFTSADDYIFRYADQAIAHSAISLLMPV
                     RMDEYRRRELHPIFQMNLPEGYVLEQLRNRLAKTVNVDPMLLLALSGSSSPIGRVFVS
                     SQAVDALLMRQQFPGERLEEILTWDGAEDIFVDLVDRYILRAGISGVQPKVLVPEHAE
                     SVPHKFTAKTSDLIIKSGREEFPALAINEFLCMSIAQEAGIPTPEFYLSDNARLFVMR
                     RFDRDEQHNPIGFEDMAALMGLSAEQKYSKSYAAIAKAIRLYCPTEHLRSSLDQLFDS
                     VALSCIVGNGDAHLKNFGLLYSDPTQRDARLAPAYDIVNTTAYIPDDVLALDLLGNKS
                     LFASRQGLLEFAKTCDVDQPKARILRLLAAQEVVLARFPELCEQAPQVAAAIRASAQP
                     FIKTFG"
     gene            complement(16217..17254)
                     /locus_tag="VFF22_20385"
     CDS             complement(16217..17254)
                     /locus_tag="VFF22_20385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012724844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HZX19212.1"
                     /translation="MTVKTPWYECDSRFIAAHYQPAALIDLAISREIDTHRLLRGTGL
                     FYDDILAGQMRISPQQFLGLIDNAQRLLEADDTSFLFGQRLLPGHYGAASQALQQAGN
                     LQQALELLIEMRALFSPLLAPRLLLDEQHAYLYWVDSSGAGPHWRFLIEACMTAVVAM
                     ARWLSGQQLPWRFHFAHAQPRYIEQYWVHLGEDLHFDSQLDILRLPREFMTAAWPNAA
                     HTAGAVARQEATRQLHGLGYQASLLDRLYAWLQANVRQSPSLEQAAHAFEMSPATFKR
                     KLQKHGTHYQQQHDRVRKHVALYLYRIKAYSNEEIAEFLSFHDATNFRRSFKRWTGLS
                     PSALRQLFEAR"
     gene            complement(17265..18182)
                     /locus_tag="VFF22_20390"
     CDS             complement(17265..18182)
                     /locus_tag="VFF22_20390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZX19213.1"
                     /translation="MPPLLKLHVWKLTGLLLAANFGLLAYLGAGTLKALGEWNWTDIG
                     GEGGSALLVLVWIGLLLKSRPAGQVTTLLFYGLACLFFSLWMDTLDEFVQLSESIAWD
                     KWLESGPMPVGFILMTLGIYHWHREQLAISGQMEKRERLFREHRLFDKVTPLGGADYL
                     RLQLQQALQEAHSEQQPLSLVILDLDDFSRVNREYGHAEGDRVLQVVSQVMVLNLRRQ
                     DLLCRLAGDRFVVLLPNTGEQQARHLALELQAAMQHLAYKSHLHGERLQLSASVAVVM
                     AQQDSPEGLLQRLNLALARAKQPPFIKRA"
     gene            18584..>19125
                     /locus_tag="VFF22_20395"
     CDS             18584..>19125
                     /locus_tag="VFF22_20395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HZX19214.1"
                     /translation="MKVRSSSARNSGLHISVLALAIASSSQLMAAEPATEYVEVVGQA
                     VSIDKALKEQRSSDNVESVVHADGIGQLPDDNAAEALQRIPGLSVERDQGEGRFVSVR
                     GIGPDLNSVTINGTLVPSPESDRRAVALDVLPSELVQSLTVVKSLTPDMDANSLGGTV
                     EVESLSAFDHEGLFYTGSAEG"
BASE COUNT         3844 a         5490 c         5925 g         3866 t
ORIGIN      
        1 cgacgcggtg gccgcggatc ttgatctgtt ggtcgcggcg accgagaaaa tccagctggc
       61 cgtcggctcg tcggcgagcg aggtcaccgg tgcggtagag acgttggcca tcaccgatgt
      121 gcgggtagcg gacgaaagcg ctggcggtct gttcgggcag accgaggtag ccgctggcca
      181 gggtcgggcc gcccaggtgg atttcgccag gctcgccgat ggccgcctgc tgggcgctgt
      241 cattgagcag ccacaactgc atgccgggaa tcggcttgcc gatactgatg gaggcatcgc
      301 caggtgctgt gttgctaagc acctgcgcag tggcccatac ggtggcttcg gtcggcccgt
      361 actcgttgac cagttgcgcc tgcggtgcct tgctacggtg cttctccagc acccgtgccg
      421 tgcagggttc gccagccacg atccaggtac gcaaatgggc aagggctggg gctgggcagt
      481 agtccagcag cgtctcgtac agcgacggca gcgataggct atggctgata cggtgccgtt
      541 cgatcagtcc ggccagggcc tctaggtcga gctcttcgcc gctcgccggt agcactagca
      601 agccgccctg gcagagcgtc cagtaaatgc cggccaccga gctgtcgaag gacagcgacg
      661 aaagcagcag atagctctgc accggctccg ggtagtacgc catgcgcacc tgcgtcgaat
      721 ggctcaggct ggcgtgactg attggcacac ccttgggttg cccggaggag ccagaggtgt
      781 agatcaggta ggcggtctgt gttggggcga cagcaggcag cgaaacagac tcgggcaatt
      841 caccatgcgt tacgtccagc agcagagttc gggcgctgct ggcgacgctg ccctgctgct
      901 cgcgggtgct gagcagcagg cgggcgccgc tgttctcgat catgtaggcc aggcgttcgg
      961 ccgggtagga cggatccagg ggcaggtagg cggcgccggc tttcagtgca gcgagcatgg
     1021 cgactatggc ctgacgttcg cggcgtaaaa ggatgccgac cacatcgccg gtctgcacgc
     1081 ctgcggcaca cagacggcgg gcaagcgact cgacctgatc gagcaattcg ccgtagtggg
     1141 ttatgcctcc cgcatcggct agggcaggcg cattgggggt ggctctggcg cgttcctcga
     1201 tcagcccgag tacgccgatg gcacgaactt gcgcctgtgc agcgttgccc agcagctcat
     1261 cttgccgagg gccgacgagt gacagcgcgc cgattggctg ggctggcgcg cacaggccgc
     1321 gcagcagtag tgtccattgc tcctcgatgc aggcaatggc ggtggtatcg aagcgcgtcc
     1381 ggtcataggc caggcgcagc tccagcgtcg ccgcatccag tcggcattcc aacccgagcg
     1441 cgaagcgctg gcgcaagctg ccggcgtcta acagctcgcc atggctttgc aatgacgctg
     1501 cgtctctaca ggtgaagaag acttcgcaga cctgttcgct gtcgtagtaa tcacgccagc
     1561 cgcgagcctg gcgtagctgg ccgagcagtg tggaggcctg tgccttgagt gacgcgtgta
     1621 ggtcgacctc tgcctggatc ggcagaacct gctcgaacag ccccagcgct tgctccagcc
     1681 cttcgccacg gccggcgtcg caccagccga gctgaattcg cggctgcccg ctgagccgtg
     1741 ccagcagtac gcaccaggcg ctgaagagcc attcctccag gctcatgtca gcctgttcgg
     1801 cggccagctc cagttcgcga cgtgtgggca cgttgtgcaa tggctgaagg gctggttggt
     1861 agagcgcgga ggcagcgctt gtctgcaaac cgtgcagctc aagcggggcg ctttccgtgc
     1921 gtgcctgttg ccagaagcgc acggcttggc cctcagcctc ttcctccagc aggctgtttt
     1981 tccactccgc gtagtcgacg tactgcaggc gctcatcggc aggcgcgccg agcagttcgg
     2041 cggcgatcag ctccaggctg gtttgatcca gcagagcggc cgagcctttg agccacagcg
     2101 tccagcgctc atctgccgtt cgtgcgtagc agaccgacag caccgactca tccacttgct
     2161 cttcgcggta tcgcgtcagg cgcgccccgt gttcctgcgc gcaaagccca cgccagtctt
     2221 cgacgacgaa ggtgacacgg gccacgtcct ggatcacttg gatcggcaag gccatgcccg
     2281 gtaccgcatg caatgccgta cgcagaattt cctcgcgctc gcccaaccct tgtaggcgtg
     2341 cctcgatttc ggcgggcgtg ggagcctgcg ccggttcgat cagcaaccag cttgcggcgt
     2401 tggccctagc ctgttgctga gtatcacgcc aggctcgggc ctgttgtggg gagatgcgaa
     2461 aacctgtcag agccgacatg ctgtttcctc gtgagcaaag gggaagaagg ggcgtatcag
     2521 agctgtacgt cgttgccggc cgtggcttcg gccatgccgg tgtagatctt gcgggggccg
     2581 ctgtagggct cccggccatg caccgcgagc atgttgtcga gcagtaggat gtcgccgcgc
     2641 tgccagcgga acttcaccag cgagcccaga taggcggcac gcagcgtctc cagcacatcg
     2701 ctttctatgg ggctaccgtc gccatagaag gtgttggtgg gcaggtccat cggcgagaag
     2761 cctgcgtcga gcgagtcgcg gatggccgcg ggcagcgtac tgaggtgaaa gaaggtgccg
     2821 tggttgaacc agacttcttc gccggtacgg ggatggcgtg ccagggcgga accgacctgg
     2881 cgggtacgca ggcggttgcc atccttccat tccacttcga tgcccacgga ggcgcagtag
     2941 gtctcgactt ctgcgcggtc gtcggtttgg aacacggact gccacggcag accgaagcca
     3001 tcgccgtagt tgcgcacata gagaatcttc ttctggcgga aggtctcgat gatttcaggc
     3061 gggatctgcg cttttaccgc gcgcactgaa ccgatggggg tttcaccgcc aatttccgag
     3121 ggttgatggc aatagaagaa cagccgcagc ggaaaacgtg gggaatagga gtgttcgttg
     3181 tgcggaaaga tgacctggtc ggctggatag tccgtcgagg tgtagatatt gccgcccacc
     3241 cgagttcgcg gcgaggcgcg gaacatgtac tccagcgcgc cgctcgacag ggcgtcgatc
     3301 acggcgttga agtgcgcggc ggcctgtacg tcgaagccgc gcagcagaat ggcaccatgt
     3361 tgcaacagct tctgctccag acggtcgcgg ttctgcgcgg cccattgcgc cagatcgacg
     3421 ccaggcagat tcggctcgac cagccaggga agatcccagg ccggatctag taggcgttcg
     3481 ctgacaatgc tctgctcgga cagcacgatg ggcttgcgac gggcgatccc ggtgctggga
     3541 cgattgccag aaggtggagt ggagatcatg cgggttcctt gtcgctacag gctgacggcg
     3601 cggcgctggg tgtttcccag gcgggcgcgg ccttgattgt tcagcgtgtg ttgctgttgt
     3661 tgcagatggg cgtcgtccca gttctgcagg cgttcacgca gcatgctgat cggtagttcg
     3721 agcagggtgt cgagcgcccc cagcaggcgt tgccagaggt gagcgaagcg ttctatctca
     3781 tcggggctga acaggcgccg gtcgtattcc agggtgacct cgatgctgtc gtggcggtgc
     3841 atcaggtcga gcaccaggtc gaagccggca agatcctgaa tgacctgagt gcggacgatt
     3901 cgggcgtcgc cgagcgtatc gatgctgaaa cggtcacgct ggtagttgaa cttggtctgg
     3961 aacagcggcg agcgtcccgc ctctcgctgt ggcgccaact gggacaccaa gaggtcgaac
     4021 ggtaattggc ggtgcgcatg ggccagacgg atcgcgtggc gggtacgcat aagcaattca
     4081 cccagaggca gaccgtcttc gagttcacac ttgagggtga gctggttaac gaagaagccg
     4141 aagatgtcct gcaactcagg caggtcgcgg ccatgcacgt cggtgccgat cagaaagcgc
     4201 cgctggccgc tgaggtagtg cagccataac tggaacagtc cagtgagcat cacaaatagg
     4261 ggcagtcgtt gagcacggct agcccgcttc aggtgcgcga cctgcagcgg ctggatcacg
     4321 aactggtgtc gtcctccctg agtgtctcca tggggacgtg ggcgtttgct gaagtccagt
     4381 tgcaatggca tgccggctag atgatcgcgc cagaacgcag tttctacctc aatcaactcg
     4441 gcggtgacat agcccggctc ccataccgcg aagtcggcgt actgcaggcg tccgggcagc
     4501 agtgcgccgg gttcctgggt ggcgtagtag tgcgccagat cctgtaggag gatttcgccc
     4561 gagcggccgt cgaaggcgat gtggtgaata acgatgatca gctcttgcac gttcaggcca
     4621 agatcgagca aacacgccct tatcaccgga cctgactcga gatcgaacgg cctgttctgc
     4681 tcatcgtgca tcagccggtc aatcgcctgc ttctgctcag ccgctggcag gttactgagg
     4741 tcgcgttgtt ttacctcgat cgattggccg gcagccaacc actgttcggg gcctgccggc
     4801 gtcatgcggt agcaggcgcg cagaagtggg tgtcgaatgc acaatgcatt gaatgcgtgt
     4861 ttcagccgag tctcgaccag ctgcggacca ttgagcacca ggcggaccag gatattgtgc
     4921 tgagcgcgac caggatccat ctggtgcaga aaccacaggc gactctgcgc gaatgactgt
     4981 cggacgcacc gggcagccga ttcgctcaaa ggcatgagag gagggcaggg cttaaccatg
     5041 catcccccta ggccattgcc acgatgacct tgcggtcacc gctgaacggg ttgcggccgt
     5101 gggcgaccag caggttgtcg agcatcaaaa tgtcgccggc ctgccaggca aaggtcacgc
     5161 tggctctggc gtagaccgct cgaatggcat ccagagtggc gtccggaatc ggtgtgccgt
     5221 cgccgaagta gacgttgcgc ggcaattgcg ctgggccaac cgcctcgatc agtgtctggc
     5281 gcagatcagc ggacatggcg gagatatgaa acaagtgcgc ctggttgaac cacacccact
     5341 catggctgac cgggtgctgg acggcggcct ggcacagttg cttcgtgctc agctcgccgt
     5401 cagcgttcca gctccattca attccctgcg ctcggcagta ctgctcgacc ttgccgcggt
     5461 cgtcggtgtt gaacacctgc tgccagggaa tatcgagcgc gctgctgtag ttgcgcacat
     5521 agagcagccc cttggcgatg aattcctcgc gaatcgtcgg gtcaatttcc cggtacacct
     5581 ggcggctgtc agctatcggg gtttccccac cgctctgact cgccgtcatg caatggaacc
     5641 aaatgcgcga tggccactgg cgcgtgtagg actgctcgtt gtgcagggga atgctctggt
     5701 gagccgggta ttcggtagag ctatagaccc ctgcgaacac cttgctccgg ggcgtcgagc
     5761 cgaactcata ggttcccagt ggcatgccga aactggcagc gaaacgtttg aaatccagag
     5821 gtgtggggac cttgaagttg cggaacagga cgccgccggt cgtccctaac gcgtccaggg
     5881 ctatggcgcg aatctcgtcg ctcattgcat ggatcgagtc ccgctgctgg ctattgatga
     5941 gcaggggcag cgcgtggctg tggagcagag gggcgataac gcagtcatct tgttccaagg
     6001 gtgagcggat cgcatcagta gcgcacacat ctgcaagctg actcatgcgt gggacgccct
     6061 cgcgccagag cacaactgat gtggaggcgc tgtaagtacc cccgaaacat agttcgcaat
     6121 aatctgagta gtggttggtg cgcttccgtg tagacgatga gccatggctc ctccctccaa
     6181 tcaagaggtt tgtgtcaact ggcgcgccga cctattcagc ttcagcgcaa ccgccttccc
     6241 agcatgacta ccctgcatgc aatgggattt aaatgataat cactctcgta aagattgcaa
     6301 attattcttg ctatcaatag tatttatcaa ctgtgagtgt ctggcttctt tagtcggagc
     6361 tttggggagg gagggagcgg tctattgggg gcgctccgga cgaggaaatg gcgaaggagc
     6421 caagtggcat atgaataaat ttaagaaaaa tacgctggcg ttggcggtta cgtccggtct
     6481 gatggtagcg acttccgcgc tggcggccga ggtagccgaa aaagaacaac cgctgcaact
     6541 gaaagaagtg gttgtcactg gcgagaagat tcagcgcact caggacaaaa cgctgtccag
     6601 cgttgcggtg gtgaccagtg aagatatccg tactcacggt gaccaggatt tgcaaagtgt
     6661 gctggaccgt actccgggtg tttacgccca gtcaggtaac gaaaattggg gtatccgcgg
     6721 tattccggta tctggcttcg atgaccaggg accggcggcc atgaacggtg cggtatcggt
     6781 ctatgtcgac gatgcgctgc aggtcaatcg cctgctgacc ctcaacccac taccgctctg
     6841 ggacatggag caagtggagg tctaccgcgg cgcccagtcg accacccagg gccgcaattc
     6901 cttggctggc gccatcatca tgagaaccaa gaacccgact ttcgatccgg agttctccgt
     6961 ccgcggcaat gtcggcaagt atggcgagca aggcacgtcg ttcgttgcca acggcgcgtt
     7021 ggtcgaaggt gtgatagcag ccaggttggc cgtcgattat cagactgccg acggccacat
     7081 cgaaaacgaa actctggata tggacgccaa ccagctgcgt tcggccaata tccgcagcaa
     7141 gttcctaatc cagccgtccg atgacctgga tgtgctgctg accttcgccc gtacgaagca
     7201 gcgcagcggg gttaacgctg ttgcagccaa taatggcagc ccggattatt tcagccttta
     7261 cgaaaacact gagacccaca acacgctcga gcaggatacg gccaccgcca aggtggatta
     7321 tcgtttggac gatttctgga ccctgaccag tgttacctct ggcacttggt cggaatacca
     7381 ctccatcctc gatttcgacc gcttaccgac tgcgacccag acggctcccc gtcatcacga
     7441 acagacgcta ggcagccaag agctacgctt gggttatgca tcggagcagg taaacggcgt
     7501 aattggtgct tacctaggcc gctttaaggg cgagatcgac gatcagcttg tcaacaatgg
     7561 catggtccta ctggatcagc agggcgagac caagatcaac agccaggcac tgttcggtga
     7621 gctcaactgg gagttcgtcg aggattggca actgatcacc gggttgcgtt gggatcatga
     7681 gaaaaacaat accaagatcg aataccccac cgatgtgttg aacattgcta ctgacccctc
     7741 ggcagacgaa gacatcagtt ccagcgtact gctgccgaag gtcggtctga gttatgacct
     7801 gactgacaac catgtgcttg gtctgacctg gcaacgcggt tatcgcagcg gcggggtaaa
     7861 cgtcaggact cgtgcaaggc atgcgaccta cgatcctgaa ttcaccagca cttacgaact
     7921 ggcctggcgc ggtaactggc tggacagacg tctacgtacc agtgccaacc tttatcgcac
     7981 cacctggaaa gatcagcagg tgtcttttct caatctcagc gataacacgg tccaggttgc
     8041 caacgctgca gagagccgca tggtcggcct ggagctcact gccgagtatc tggtgacttc
     8101 gcaactgctg ctgaatgcgg ggctggccta caacgacacc aagtacgagt ccttcgtgcg
     8161 tgacggtgtc gatctcagcg accgcgaatt cgttttcgcg cctaagaaaa tggccaatat
     8221 cggagccaac tataccttcg gcaacggctt gatgattggc ggtgatgtcg tttaccaggg
     8281 caagagcatt tccaacttca acacgagcgg atcgactgta accggcgaac gctacaacga
     8341 cagcgtcgcg ctggtgaacc tgaatgccga gtaccccctg ggtgacctga cgctcagcgg
     8401 ctatgtgcgc aacctgttcg acgagcgcta tatcactaac aaccagagcg acagcctgct
     8461 agacgtcggc gcaccgttga ccgttggcgt ggccgctcgt tacgacttct gagaaacact
     8521 ggcggcagtg caacgtgctg ccgccagata tggaattcaa ggtttgttgt cttgtcttgc
     8581 ccggcttgcc tgggtgagtg cttagagaac gtgccattcg tgtgatgcga ggatgcaatc
     8641 atgagtgtat tcagcccgat aatgagcgcc actgccgacc ttcagcgtcg gcattcagga
     8701 ctcagagagc gctactgcct ggacaccacg ccactcttgc agcgacaaat ggagcgagag
     8761 tcgaatgctc gtagttatcc acggcgaatc cctctggaac ttaaacgtgc ccaaggtatt
     8821 tacgtcgagg actcgagagg gcaggtatac atcgactgcc tggctggcgc aggaacactg
     8881 gcactggggc acaatcatcc cgtgacggtg gaggcgatac aaagagcact gagttcgggc
     8941 gtccctctgc ataccctcga tctgagcacg cccatcaagg acgcgttcgt gcagacactg
     9001 ttcgatctgc ttccaagcga tttcgccaag aatgcccgta tccagttctg tggtcccagt
     9061 ggagcggatg cagtcgaggc cgcaatcaaa ctttcccgta ccgcattagg tcagcagacc
     9121 attctgtcgt tccacggtgc ttatcatggc atgacccttg gaacgctctc gctcagtggc
     9181 aatctaggcc cgaagaaatc actgggtgga ctactggcag gtgttcagtt cttaccctat
     9241 ccatacagtt atcgttgtcc cttcggtctg tctggagaac agtctattga tgccggcttg
     9301 cattacatcc ggcacctgtt aagcgatcct gaaagtggta ttactcagcc ggcagccatg
     9361 attctcgaaa cggttcaggg tgagggaggg gtgattcctg cccctgaacg ctggttacag
     9421 gggctccgga acattactca ggaacatggc atcacgctta tcttggatga gatccagtgc
     9481 ggtattggcc gtacaggtga tcttttcgcg ttcgagcaaa gtggaatcac accggatgtt
     9541 attacgctgt ccaaggccat cggcggcggt ttgccgctct cggtaatggt ctatcgcaat
     9601 gaactagacc aatggcaacc aggcgcgcat gccggcacat ttcgtggcaa tcaactggcc
     9661 atggcggcag gcgatgcgac actgcggttc atccagcagg agcaattaac cacccatgtg
     9721 cgatgtgtgg gtaatcagct gcagctttta ctacaagggt tacaagccga attcgattgg
     9781 gttggtgatg tccgcggacg cggtctgatg ttgggcatgg aaatcgtgga ccccgaaagc
     9841 tccgattccc tgggacggcc tgcggcggat ggtgacaagg cgcgcaaact tcagcgtgct
     9901 tgtctggagc gcggcctgat cgtggaacta ggggggcgac atggcgccac cgttagattt
     9961 ctgccgccgc tgattatcag tgaaatggaa atcgagcatg tcggacaaat tctttatcag
    10021 gcagcgcaag ctgttcatgc ggttttcagc tgagattggg ccctcagagg taggtctcgt
    10081 ttgcagaagt caaattcctt taaccacgtg ccttggaaag ccctaggtat atgacctcag
    10141 gttggaagga agccaaatct gcttatctgc acatcacttg ggtgttgcca ggcggctcat
    10201 caaagggatt atgagcaatg caaggcccgc aacagccgcc gctacatagc caacgctggc
    10261 tatgcctgcg gtctggatgg tcaaaccgcc gatgatcgcg ccgatgccta tccctgcgtt
    10321 ggctgcagaa acgttggtcg tcccggcaag ggcttgcgag tggctcaccg agttcatgac
    10381 ccgcacctgg cagatcggat aaagcgctgt attggcgata ccccagagtc ctaacgcgat
    10441 acatatgagt agacctgcgc tcgccaaggg cgtgactgca gccatgcccg ccgccagcag
    10501 caccacgaat agggccgtgg ctttgagtgg cgagcggtcc actgctttgc cgccaatcca
    10561 gttgccaacc aggcctatgg cgccgaagcc catcatccac cagcccacat gcgcagggtc
    10621 cacgccggcg atgcgctcca gcatatctgc caggtaggtg taggcaatga acatggcgct
    10681 gaagatgatg atcgacaacg cgacgttggc cagaaacggc ggctctttga agatgcgtgc
    10741 ttgtttaaga aagtcgaccc gtgcgctgcg tgcgactttt ggcatataga tcgccatggc
    10801 aacggccatc agcagtgaca agcccgccag cagccagaag gcgcctcgcc agccgatgcc
    10861 gttggccgcc agcgtaccca ggggaatgcc cagcaggagc gcggcagtga tacccaggta
    10921 gacccgggca atggcttggc ctgactttgc cgggccggcc agttgggctg ccgtttcact
    10981 ggccgtgccc cagaacaccg gcaacatcaa tgccgggata aaacgcgcaa tcgccagtac
    11041 ccagatgttc gatgacagtg cggccaggcc attggcaaat gcgaagacca gcagtatcgc
    11101 gataaacagc ttctttctgt ccaggtgggc gagggcggcg gtcagcggtg ggccaaagag
    11161 catgacagtg aaggcgaaca gcgtgaccaa ctgacctgct gcggcaatcg aaatgccaag
    11221 gtcacgggcc agggcgggaa gcaggccgat aatcaggaat tctgtggtta cgatcagaaa
    11281 ggcagagatg gccaggatgg tgatgctcag cgtggaacta gccgaagtgc aaattagtga
    11341 ccggggtgat tcgatagcac tcatgacgcc gctcttcagt aaggaaggtg tcatggtatt
    11401 ggttcttaat tttcctattg ctggtgtttt ttctggctat tgctgagcta aattcactta
    11461 tccgcttgtt caggcaagtt gtggcctgcg ggagtcaggc agatcgcgag cgcttccagc
    11521 aggacgctga ccttgggcag cgtctcgcgt ttctttagcc aaaccaggtt cattggcagg
    11581 ccatcggtgg ccagctgcgg cagcacctcg accagtgcgc cgctatccag atgcccctgc
    11641 accagccagg taggcaattg cgcgatgcca tagcccgcca ggacggccag cacttccccc
    11701 tcaccatcgc cgacagcgat tcgcgccggc atgattcgcc gcttcttgtc gccaggcgca
    11761 gggccttctc ggtaccaggg gctgacctga ccatcgctat ggccgtaaag cacgcagtcg
    11821 tgttgctcaa gttccgcttc gcttgccggc gtccccctcc tagaaaggta ctcgggagcg
    11881 gcgcagtaga tcaggcgctc ggcgcccagg tagtggtggc tcagggtcgc cggccaaata
    11941 tccgaaccac cgatgcgcac cagaatgtcg atgccttgat cggccgggtc gacaaagcta
    12001 tcggtaaagg agatatgcgg catcagcagt ggatgctgtt tggccagctg cagaatcacc
    12061 ggcagtacat gcatgcgccc ataggacgcg ggcagatcaa tacgaattcg cccacgaggc
    12121 tcgaagaact cggcatgcag ggccatctca gattcttcca agtcggcgag tactcgcgtg
    12181 caggttcggt agaaggccgt acccgcatcc gtcaacgcca gcttgcgcgt ggtgcgttcg
    12241 aaaaggcgta tgcccaggcg ggtttccagg cgcgcgatgc ctttgctcac cgccgagctg
    12301 gtgaggctca gacgttcggc cgccgcagca aagctgccga gttcggcgac gcagacgaaa
    12361 acatcgatgc ctttcaaacg ctcagaggag aacacggcat gaccttgaat agctgtgggg
    12421 cggggcaggc aaacctgacc ttcggccaat ggtcgttgat cagggctgcc aggtacagcc
    12481 tttgcgcagg gttaccccag cgtgcagcgc gtcagcgttc tgctgtattc agaattgcct
    12541 gctgatagcc gcgaccaata tgcctgcagc gatcgccggc agaggtttct ttaccagtag
    12601 catgacgatt gccgtggtca gcaaagccag ccgcgcactc atatcgccat caatcgccag
    12661 gggggtaaga atggccacca gcaccgagcc ggacatggcg ctgatgaact gctgaaccct
    12721 gtagctgatc ggcacatacg acatcacgaa cacaccaccc cagcgggtcg ccagtgtcac
    12781 ggcagccatg atcaaaacga taatcagcgt gccaaaaccc gtcgtggcaa tgctcatttt
    12841 ttcttctcca tccacagagc acccagaacg ccgcctgcca gtgcgccgac aaccacatgg
    12901 ctattgtccg gcagatacca gtaggccagc agcgatgaac cggcggcgac tgtccagatg
    12961 agcaacagtc gcagattttt ctcgcccccc acggccatcg ccagcaggaa gcagcccatc
    13021 accatgtcca ggccgaaact cttggggtca tggatggcac cgccaaaata gatgcccagc
    13081 caggtgccga atatccagaa tgaccacagc gcaagaccgc cacccagcaa taagccgaag
    13141 cccggtttac cgcgattgaa ggcttgtatg gacagggccc agttggcgtc ggaagcgacc
    13201 agcatcacgc catagcgttt tgccggtgat aaatggcgca gccagggata gagcgatgcg
    13261 cccatcagca ggtgacgcgc attgatggca aatacggtga gggctagcgg gaccagggga
    13321 atttgcacgc cccacagctc cagcgcggcg aactgtgagg cgccggcgaa gaccagcgtg
    13381 ctcatcagca ctgccgaagg attatccagc ccagtctgta cggccgcgag gccaaaagcc
    13441 gcgccgaagg cgataacaaa aatggaaaga gggacgagtt gcttgaagcc tgcccacaca
    13501 tcctggcggc tgaactcatg caactgtgtg gcgctgtccg acatgttgca gcctctgctt
    13561 cgtttcaagt ctgtttgttg ccgatgtggc ctggtgcttc ctcactggtc agtgggcgaa
    13621 gccagttaac gcacggcaga actatgcacc tgacgctaac tggagaaaaa cgcataattc
    13681 agcagcaagc cattcgataa gctaatgcat gagttgcccc atgaatgttc aagacctcca
    13741 gattgactgg cttaaatgct ttgtcgccgt cgtcgatgca ggctcgttgt cgggcgcggc
    13801 cccagaggtc aaccgctctc agtcggcggt gagcatgcag ctcaagaagc tggaagcagc
    13861 tcttggctgt cagctgctaa tgcgcggacc ccggcaccat caactcacct cagaagggca
    13921 actgctgctg ggctatgcca ggcggatgct cgacctgcat gccgaaacac aggcggcctt
    13981 tctgggggag gagcttacgg gccggattcg ccttggtgtg ccggatgact atgccgctcg
    14041 ctaccttact ccagtgctca agcgtttcgc gccgcagcac ggtgcggtcg agattgagct
    14101 ggcatgcgag caatcaacct cgctaatacc ccgggttgag cgcggtgatc tggatctggc
    14161 attggtctcg cgagaccagc tgcggcacgg cactttgctt tttcatgagc cgatggtctg
    14221 ggtcggctcg gcgcagtttc aggtgtggcg gcgcgatccg ctgcctatcg ccgtctatga
    14281 agacagcagc ctcgccaagc gcagcgcgat taactcccta gccttgcagg gccgtcgtta
    14341 caaggtggtg tacaacagct ccagcctggc ggggcagatt gctgcggttg aaagtggcct
    14401 ggctgtcgcc gtgttgaccc agtgcagcgc gccgacgcac ctggagatac ttggcagtga
    14461 ccatggccta ggcccgctgg aacccatgga agtggccgtt tatcgaagcc gcgtttccca
    14521 gggctcaaaa gccgtcgata gcctgcacag cctgctgatc agaactctgc ggctttcaac
    14581 ctaagcagta acttcgccgt ttgaatgaca gcaggcttac tgcgtgcagg ggtaattgag
    14641 tggcatatgc ccccctagat ggcgacttga tcttgacttt agtttcgtat acgttacatt
    14701 taccgataaa ggttatgtat acgtgacatg gactatcttc ttctgacaca gcgtttaggc
    14761 cagcaaatcc gcgagcggag acttaaccgt gggctgactc aagccgggct ggcggaaatt
    14821 gccgggctta cgcggcaaaa aatcattgcc gtggaaaagg gcagcatttc tgttggcatg
    14881 acggcttatg cccgcgtgct aggtgccctc ggctgtgagc tgtcggttat tcccgcaagc
    14941 atgccgacgc tggaagaagt gcagggggtg tttgaatgag cgcgccattg caggtcagca
    15001 cgccggcagg cgatagcgga cgaatcttca ctagcgctga cgattatatt ttccgctatg
    15061 ccgaccaagc gattgcccat tctgcgatca gcttgttgat gcccgtgcgc atggatgagt
    15121 atcgccgccg cgagctgcat ccgatttttc agatgaatct tcccgaaggc tacgtgctgg
    15181 aacagctgcg caaccgcttg gccaagaccg ttaatgtcga ccccatgctg ctgctggcgc
    15241 tctcaggcag cagctcacct attggccggg tctttgtgag ctctcaggcc gtcgatgcgt
    15301 tgttgatgcg ccagcagttt cccggtgagc gcctcgagga aatcctcacc tgggatggcg
    15361 ccgaggatat ttttgtcgat ctggtcgacc gctacattct gcgcgccggc atttctgggg
    15421 ttcagcccaa ggttctggtg ccggaacatg cggagtctgt gccgcataaa ttcacggcta
    15481 aaacctctga cttgatcatc aagagcggcc gcgaggagtt tccggctctg gcgatcaatg
    15541 aatttctctg catgtcgatt gcccaagagg ccggcattcc caccccagag ttctacctgt
    15601 cagataacgc caggctgttt gtcatgcgcc gctttgaccg tgatgagcag cacaacccta
    15661 tcggttttga agatatggcc gcgttgatgg gcttgtctgc tgaacagaag tacagcaaaa
    15721 gctacgccgc catcgccaaa gccatccgtc tgtactgccc gactgagcac ctgcgcagtt
    15781 cacttgatca gttgttcgac agcgtggccc tgagctgcat tgtcggtaat ggtgatgcgc
    15841 acctgaagaa ctttggcttg ctctattccg acccaacgca gcgtgatgcg cgcttggccc
    15901 cggcctacga catcgtcaat accaccgcct atatcccgga cgatgtgctg gcgctggatc
    15961 tgctcggtaa caagtcgctg tttgcttcac gccaagggct gctggagttc gccaaaacct
    16021 gtgatgtcga ccagcccaag gcgcgcattc tgcgcttgtt agcggctcag gaagtggtac
    16081 tcgcacgttt tcccgagctc tgtgagcaag cccctcaggt cgccgcagcc attcgggcaa
    16141 gcgctcagcc gtttatcaaa acgttcggct gaagatgatc agcgccgccg ggcatttatc
    16201 gtctgagctt taccagctag cgcgcttcaa ataactgtcg cagcgcgctg ggcgacagcc
    16261 cggtccagcg cttgaaggag cgacggaagt tggtcgcgtc gtggaaactc agaaactcgg
    16321 caatttcttc attgctgtac gccttgatcc gatagaggta gagcgccacg tgcttgcgca
    16381 ctcggtcgtg ctgctgctga tagtgcgtgc cgtgtttctg cagtttgcgt ttgaaggtcg
    16441 cggggctcat ttcgaaggcg tgcgcggcct gctccaggct aggtgactgg cgcacgttgg
    16501 cttgcaacca ggcatacaaa cggtctagta ggctggcctg gtagccgagg ccgtgcagtt
    16561 gccgcgtggc ttcctggcgc gccaccgcac cggcggtgtg tgcggcgttg ggccaagctg
    16621 cggtcataaa ttcgcggggc aggcgcagta tgtccagctg gctatcgaag tgcaggtctt
    16681 cgcccaggtg cacccagtat tgttcgatat agcgcggctg cgcgtgggcg aagtggaagc
    16741 gccacggtag ttgttgcccg gacaaccagc gggccatcgc caccacagcc gtcatgcagg
    16801 cttcgatcag aaagcgccag tgcgggccgg cgccgctgct gtcgacccag tagaggtagg
    16861 catgctgttc atcgagcaac agccgtggtg ctagcagggg gctgaatagc gcgcgcatct
    16921 caatcagcag ttccagcgcc tgttgtaggt tgccagcttg ctgcagcgcc tggctggccg
    16981 cgccgtagtg gccaggcaac aggcgctggc cgaagaggaa gctggtgtcg tcggcttcca
    17041 gcaggcgttg tgcgttatcg atcaggccca ggaactgctg cgggctgatg cgcatctggc
    17101 cagccagaat gtcgtcgtag aacaagccgg tgccgcgcag caggcgatgg gtgtcgatct
    17161 cacggctgat ggccaggtcg atcaatgccg caggctggta gtgggcggcg ataaagcggc
    17221 tgtcgcattc gtaccagggc gttttcacgg tcatggcatc cgcctcaggc tcgcttgata
    17281 aaaggcggtt gcttggcccg cgccagcgcc agattgagcc gctgcaacaa gccctcggga
    17341 ctgtcctgct gagccattac caccgccacg ctggcgctca gttgcaggcg ctcgccatgc
    17401 aggtggcttt tataggccaa gtgctgcatg gcggcttgca gttccagggc cagatgccga
    17461 gcctgttgtt cgccggtatt gggcagcagc accacgaagc ggtcgccggc caggcggcag
    17521 agcagatcct ggcggcgcag gttgagtacc atcacctggg acaccacctg caatacgcgg
    17581 tcgccctcgg cgtggccgta ctcacgattg acccggctga aatcgtccag gtcgaggatc
    17641 accagcgaca gcggttgttg ctcgctgtgc gcctcctgca aggcttgctg caactgcaaa
    17701 cgcaggtagt cggcaccgcc tagcggcgtg accttgtcga acaggcgatg ttcgcgaaac
    17761 agacgttcgc gcttttccat ttgcccgctg attgccagct gttcgcggtg ccagtggtag
    17821 atgcccagcg tcatcaggat aaaccccacg ggcatcggtc ccgactccag ccacttgtcc
    17881 caggcgatgc tttccgacag ttgcacgaat tcgtcaaggg tatccatcca cagcgagaag
    17941 aacaggcatg ccaggccgta gaacagcagg gtggtgactt gcccggccgg gcgacttttc
    18001 agcagcaagc cgatccacac cagcaccagc aacgccgagc cgccttcacc gccgatatcg
    18061 gtccagttcc attcgcccag ggccttgagc gtgccggcgc cgaggtaggc gagcaggccg
    18121 aagttggccg ccagcaacag gccggtgagt ttccatacat gcaatttcag cagtggcggc
    18181 atggccaata ggctccgcag agggtaatgg ccgccagcac aacagatgca catgacggtt
    18241 tggttgcagt gggggaggtc aaattggctc aaggttgttc gccgatttat tgcgctttac
    18301 ggcgatcagg cggtttctgc cggtacgcgt tggcgcattt ctgccgcgct ataaatgaag
    18361 ggctgcgtta aacagcgtac cggcctggcg ggtagttggt cgctcggctg cgatgttgcg
    18421 gtcatttcag ctcaattgcg tgcaacagcc tgctgaaacc cgctggttta gcgctatggc
    18481 cgcaaacgct gtcacagaag cgtcatcact cctgcctagc tttcgctccc caatagcccg
    18541 gccattgcag cctggcagcg atcaatcttg gggagggtag gtgatgaagg ttcgcagcag
    18601 ttccgctcgc aattcgggtt tgcatatcag cgtgctggcc ttggcgattg cctccagcag
    18661 ccagctgatg gcggctgaac cggccaccga gtatgtcgag gtggtcggcc aggccgtgag
    18721 tatcgacaag gcgctgaaag aacagcgcag ttccgacaac gtcgagagcg tggtgcacgc
    18781 cgacggcatt ggtcagctgc cggacgataa cgccgccgaa gcgctgcagc gtattcccgg
    18841 cttgtcggtt gagcgcgatc agggtgaggg gcgctttgtc agcgtgcgcg gtattggccc
    18901 ggacctgaac agcgtgacca tcaatggcac cctggtgccc tcgccggaaa gtgaccgtcg
    18961 tgccgtggcc ctggatgtat tgccctcgga gctggtgcag tcgctgacgg tggtcaaatc
    19021 gctgaccccg gacatggacg ccaactccct cggcggcacg gtggaggtgg agagcctgtc
    19081 ggcctttgat cacgaagggc tgttctacac cggcagcgcc gaagg
//
LOCUS       DASRUM010000303         5058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1822158, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000303 DASRUM010000000
VERSION     DASRUM010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822158"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="VFF22_20400"
     CDS             <1..1363
                     /locus_tag="VFF22_20400"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HZX19215.1"
                     /translation="RAVQLNEANAFLKKHPEVLFVDLLIADMNGVVRGKRIERASLHK
                     VYERGINLPASLFALDINGSTVESTGLGLDIGDADRVCFPIPNTLCNEPWQKRPTAQL
                     LMTMHEMEGEPFFADPREVLRQVVAKFDELGLTICAAFEIEFYLIDQANVNGRPQPPL
                     SPLSGKRPQSTQVYLIDDLDEYAECLQDILEGAKEQGIPADAIVKESAPAQFEVNLHH
                     VADPIKACDHALLLKRLIKNIAYDHEMDTTFMAKPYPNQAGNGLHVHISILDKQGNNI
                     FACEDPVQNDSLRHAIGGVLETMSASMAFLCPNVNSYRRFGPQYYVPNSPCWGIDNRT
                     VALRVPNDTADAVRIEHRVAGADANPYLLMSTVLAGIHHGLTNKIEPSAPVEGNSYEQ
                     NEQSLPNNLRDALRELDDSEVLARYIDPKYIDIFVACKESELAEFENSISDLEYNWYL
                     HTV"
     gene            1562..2647
                     /locus_tag="VFF22_20405"
     CDS             1562..2647
                     /locus_tag="VFF22_20405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZX19216.1"
                     /translation="MLRLLASLLLLTPLLTVAQESAPAVERPDAVIRMYNWNDYIAPQ
                     VLKDFEAETGIRVDYHTYSTAEELEAVLASGEAIDVAVPSHDALPAMIKSGTLQPLDF
                     NLLPNRKHLDKQLLSTLVALDPANRHALPYLWGAVGLAINTPQAEAAFGGPLPNSWSL
                     LFDAEQSSRLANCGISVLDAPDETLTLLLNYQGRSLARSAPSRIERSSQVLDGLRPNL
                     RYVDSERYIDDLNQGKLCVAMAWVGDALAAADAGQPVRFLVPDEGSILFIDNLVIPSS
                     AKRVDLAHRFIDYLMQPKVAALITAETLYPSGNADSREFLDEALRNQPDLYPDRDTKR
                     RLHPLETLPEKHSAARDAVWTRFRDGS"
     gene            complement(2880..4244)
                     /locus_tag="VFF22_20410"
     CDS             complement(2880..4244)
                     /locus_tag="VFF22_20410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HZX19217.1"
                     /translation="MNKETEIKLRVSRETLAALRDHPLLKKRNKSGWEQRELFNQYFD
                     TPERDLAAAKVALRVRRDGEQFIQTLKTRGQSVAGLSERNEWDWNLAKAKLDLKKLDD
                     SCWPAALAELDKKQLVPIFTTDFIREKAEIAWGRGKAKVVIEAALDLGKVIAGEGEEE
                     ICELELELRQGEPEALLELAAELAADLALMPCDISKAERGYRLFDAGSYSLNLPAPQL
                     TGETSLDDSVAALGWHLLGSSQRLAEQYRFNGHWRLLVDWLEQLIGLRALLSSLGQAA
                     PRASSHALRELLDALLLEWRPRVLAGQDDDSLRKNAPALFAAELQGNRWGLFSLNLSR
                     WLLARSWTVERNNRGNRQGAAPLGNWLPTLIAEEGAALQLKRYVQQPEDLAEQLPRIE
                     RLLVWLHLARGVLEVPEVDRLYGELNKLAELAHQAIEPEVLDARKAQLIAIGSLKAWR
                     QLVR"
     gene            4357..5034
                     /locus_tag="VFF22_20415"
                     /pseudo
     CDS             4357..5034
                     /locus_tag="VFF22_20415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
BASE COUNT         1028 a         1654 c         1435 g          941 t
ORIGIN      
        1 gcgtgccgtt cagcttaacg aagcgaacgc gttccttaag aaacatcctg aggtcctgtt
       61 cgtcgacctt ctgatcgcag atatgaatgg tgtggtgcgc ggcaagcgta tcgagcgtgc
      121 gagcctgcat aaggtttacg aacgcggcat taatcttccg gcttctctat tcgccctgga
      181 tatcaacggc tcgacggtgg aaagcaccgg cctgggtctg gatatcggcg acgctgaccg
      241 ggtctgcttt cccatcccca acaccctgtg caatgagcca tggcagaagc gcccaaccgc
      301 gcaactgttg atgaccatgc atgaaatgga aggtgagccg ttctttgccg acccacggga
      361 agtgttgcgc caggtggttg cgaagttcga cgaactgggc cttaccattt gcgcagcctt
      421 cgagatcgag ttttacctga tcgaccaggc caacgtgaac ggccgtccgc agccgccgct
      481 gtcgccactg tcgggcaagc gcccacagtc gacccaggtg tacctgatcg acgacctcga
      541 cgaatatgcc gagtgtctgc aggacatcct cgaaggcgcc aaggagcagg gcatccctgc
      601 cgacgccatc gtcaaggaaa gcgccccggc gcagttcgag gtcaacctgc accacgtcgc
      661 cgacccgatc aaggcctgcg accatgcgtt gctgctcaag cgcctgatca agaacatcgc
      721 ctacgaccat gagatggaca ccaccttcat ggccaagccc taccccaacc aggcgggtaa
      781 cggcttgcac gtgcatatct cgattctcga caagcagggc aacaacatct tcgcctgcga
      841 agacccggta cagaacgatt ccctgcgcca tgccatcggc ggcgtactgg aaaccatgtc
      901 ggcctcgatg gccttcctct gcccaaacgt caactcctac cgtcgtttcg gcccgcagta
      961 ctatgtgccg aactcgccgt gctggggcat cgacaaccgc acggtagcgt tgcgcgtacc
     1021 caatgacaca gccgatgccg tgcgcatcga acaccgcgtt gccggcgccg atgccaaccc
     1081 ttacctgctg atgtcgacgg tactggcggg cattcaccat ggcctgacca acaagatcga
     1141 gccaagtgca ccggtggaag gcaactccta cgagcagaac gaacaaagcc tgccgaacaa
     1201 cctgcgcgat gccctgcgcg agctggacga cagtgaagtg ctggcgcgtt atatcgaccc
     1261 gaaatacatc gatatcttcg tcgcctgtaa agagagcgag ttggccgagt ttgaaaactc
     1321 gatctccgac ctcgaataca actggtactt gcacacggtt taagtaagca acaccaaacg
     1381 ccggccttgt gccggcgttg ttttatccaa ccggaactgc cgaaccagcc ttatccccgg
     1441 ccgcgcgcct tgcgccgtgg caggcattcg gctgggcgaa attcagcaga tccgggtctt
     1501 gtcgcccgca tcaccaagcc gcaccgcaag ggacggcgca gcgcttaccg ggagatccac
     1561 catgcttcgc ttactcgcct cactgctgtt actgacgcca ctactgacgg tggcacagga
     1621 gtccgcgcct gcagtcgaac ggcccgatgc ggtcatccgc atgtacaact ggaacgacta
     1681 tatcgccccg caggtgctca aggatttcga ggccgaaacc ggcatccgcg tcgactacca
     1741 cacctacagc accgccgaag agctggaagc ggtgctggcc agcggtgaag ccatcgatgt
     1801 cgccgtgccg tcgcatgacg ccctgccggc gatgatcaag agcggcaccc tgcagccgtt
     1861 ggatttcaac ctgctgccca atcgcaagca tctcgacaag cagctgctca gcaccctggt
     1921 cgccctcgac ccggccaacc gccatgcact gccgtatctg tggggcgcag tcggcctggc
     1981 gatcaacacg ccgcaagccg aagcagcctt tggcggcccg ctgcccaaca gctggagcct
     2041 gctgttcgat gccgagcaaa gctcgcgcct ggccaattgc ggcatcagcg tgctggacgc
     2101 tcctgacgag accctgaccc tgctgcttaa ctaccaaggc cgtagcctgg cacgcagcgc
     2161 accgagccgt atcgagcgct ccagccaggt gctggatggc ctacggccaa acctgcgtta
     2221 cgtcgacagc gagcgctata tcgatgacct caaccagggc aagctgtgcg tggctatggc
     2281 ctgggttggc gatgcgctgg cagccgccga tgccggtcag cccgtgcgct tcctggtacc
     2341 ggacgaaggc tcgatcctgt ttatcgataa cctggtgatt cccagcagcg ccaagcgcgt
     2401 cgacctagcg caccgcttta tcgattacct aatgcagccc aaggttgccg cgctgatcac
     2461 cgccgaaacc ctgtacccca gtggcaatgc cgattcccgc gagtttctcg acgaagccct
     2521 gcgcaaccag ccagacctct acccagaccg cgacaccaaa cgccgcctgc acccgctgga
     2581 aaccctgccg gaaaagcaca gcgctgcccg cgatgcagtg tggacgcgct ttcgtgatgg
     2641 cagttgagtg atgcgaaagg agcccggatg taatccgggc tagcgactgt aggagccagc
     2701 ttgctggcga tccgcggagc aacagcatcg ccagttgtag cccggatgaa atccgggagc
     2761 ggggccaacc gctcgatacc cccggattgc atccgggcta cggcctgcgc ttatcgcgca
     2821 gggtgcgcaa gtatccgaga gtcttggtgc gcacggcgca ccctacggcc tcacaagcct
     2881 tacctgacca gctggcgcca ggcctttagc gaaccaatgg caatcagctg cgccttgcgc
     2941 gcatccagca cctctggctc gatggcctgg tgagccagtt cagccagctt attcagctcg
     3001 ccatacaagc ggtctacttc aggcacctcc agcaccccac gagccaggtg cagccagacc
     3061 agcagacgct cgatacgcgg cagctgttcg gccaggtctt ccggctgttg cacatagcgc
     3121 ttgagctgca gggcggcgcc ttcttcagca atcagcgttg gcagccagtt gcccagcggc
     3181 gcagcgccct gacgattgcc acggttgttg cgctcaaccg tccagctgcg cgccagcaac
     3241 cagcgcgaca ggttcaacga gaacaggccc cagcgattgc cttgcagttc cgctgcgaac
     3301 aacgccgggg cgttcttgcg caggctgtca tcgtcctgtc cggccagcac gcgcgggcgc
     3361 cattccagca gcaaggcatc cagcagttca cgcaaggcat gactgctggc acgcggcgcg
     3421 gcctggccaa ggctgctgag cagcgcgcgc aagccgatca gctgctccag ccagtcaacc
     3481 agcaaacgcc agtgaccatt gaagcggtac tgctcggcca ggcgctgact gctacccagc
     3541 aggtgccagc caagcgccgc aacgctgtca tccaggctgg tttcgccagt cagctgcggg
     3601 gcaggcaggt tcaggctgta gctgccggca tcgaacagcc gataaccgcg ctcggccttg
     3661 ctgatatcac agggcatcag cgccagatcg gcagccagct cggcggccag ctccagcagc
     3721 gcctcaggct caccctgacg cagctccagc tccagttcgc agatttcttc ttcgccttcc
     3781 ccggcaatca ccttgcccag gtccagcgcg gcctcgatca ccaccttggc cttgccgcga
     3841 ccccaggcga tttccgcctt ctcgcggata aagtcggtgg tgaagatcgg caccagctgc
     3901 ttcttgtcca gctcggccag ggccgccggc cagcagctgt catcgagttt tttcaggtcc
     3961 agcttggcct tggccaggtt ccagtcccat tcattgcgct cggacaaacc ggccacgctc
     4021 tgcccgcggg ttttcagggt ctggataaac tgctcgccat cacggcgcac gcgcagggcc
     4081 accttggccg cagccaggtc acgctcgggg gtatcgaagt attgattgaa cagttcgcgc
     4141 tgctcccaac cactcttgtt gcgcttcttc agcaacgggt gatcacgcag ggcggccagg
     4201 gtttcacggc taacccgcag tttgatttcg gtttctttgt tcatgatgat tgggatcaac
     4261 gcctactatg aaattgtgac agtccgatta cctgcgctgg cgagcctgcg gactgatccg
     4321 tatacttgcc gccttcttaa cgccggggtt caccctatgc cagtcaatcc atttgccagc
     4381 ctgtttggcc gttcaccgat tgggccgatg caaaaacact ttgccaaagc ccacgagtgc
     4441 gcagccaatc tggtgccctt cttcgaagcc gtgatggttg aagattgggc caaggtggaa
     4501 caggtacaac aggaaatggc tagcctcgaa catgaggccg acaagctcaa gaaaagtgtg
     4561 cgtttgcacc tgcccaagag cctgttcctg cccgtaccgc gctcggatct gcttgagctg
     4621 ctgagtgtac aggacaaagt cgccaaccgc gccaaggaca tcgccggcct gatgcttggc
     4681 cgcgaaatgg ctattccaca ggctctgcag ccactgatgc gcgcctttgt gcaacgcacc
     4741 gtggatgcca gcgcccaagc gctgaaagcc atgaacgaac tcgactagct gctggaaacc
     4801 ggcttcggtg gccgtgaagc ggcgctggtc gaatccatgg tcatggagct gtaacagatc
     4861 gaacgcgaca ccgacaagat gcaaatcgaa gtgcgtcgtg cgctgttcaa actggagaag
     4921 gaccttcccg ctgtcgatgt gatgttcctc tacaagatca tcgaatggat cggcgacgta
     4981 gccgaccgtg ccgaacgtgt cggcaaccga ctggaacaac tgctggcgcg ctaagcgcca
     5041 gtgtcctttc tggaatct
//
LOCUS       DASRUM010000304        27083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2329750, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000304 DASRUM010000000
VERSION     DASRUM010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 27083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27083
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2329750"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /locus_tag="VFF22_20420"
     CDS             complement(<1..112)
                     /locus_tag="VFF22_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HZX19218.1"
                     /translation="MKGKSLAWALSAALAGITLSSAVQAEEKFVTIGTGGQ"
     gene            341..1366
                     /locus_tag="VFF22_20425"
     CDS             341..1366
                     /locus_tag="VFF22_20425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZX19219.1"
                     /translation="MPKHTLDSQHTSVSASITLAVLHAASRLGVDRAQLMSACELHDS
                     QLSDPDARVPFATQEQLWQQLSRIEHAEPGLALGCNLAPGPFSVLGYLLQSSPTLGDA
                     LAAGLRYQRLVGEGGELQLREQGDELQLLYRPLHPELPATRTRVLALMACWVQMLHPL
                     LDNWQLLRAQFAHEQPPTLDSYSETFACPLQFAASDYGIVLPRSLAHAPLIQANPPLQ
                     NLLRQHAEALLARLPSEGLSARVVALLGEQLARGEPDRSELARHLHLSERTLQRRLAD
                     EGCNYQQLLNDTRQQLAEQHLSTGDLPAAEIALLLGYSEPSVFFRAFRQWTGLTPGEY
                     RARQKAQ"
     gene            complement(1374..2324)
                     /locus_tag="VFF22_20430"
     CDS             complement(1374..2324)
                     /locus_tag="VFF22_20430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZX19220.1"
                     /translation="MRVLITGAAGYIGQQLLNQLAVQQPQWLLIAADIRPQCRAGIKL
                     NIEPVLLDISQPTAVEDCVATWQPQAIVHLASVVTPPPGMSEAQLHAIDVGGTQALIQ
                     AAAAHGVEQLIVTSSGAAYGYDPQNAEWLDEDQPLRGHAHFAYAKHKREVELLLAEAR
                     VQHPQLKQLVLRPGTILGKQVNNQITELFKKPAVLGIRGSDSRFVFIWDQDVVDIICR
                     GLRQQSAGIFNLAGDGALSLKEIAELLNKPYRALPAWLIRAALSVLKPLGLSPYGPEQ
                     VDFLRYRPVLSNRRLKHDFGFSPRYSSREAFLAFLRAQGI"
     gene            2530..2877
                     /locus_tag="VFF22_20435"
     CDS             2530..2877
                     /locus_tag="VFF22_20435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019361514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZX19221.1"
                     /translation="MSQAEQNLRIDYIEFAAVDLPALKQFYEQVFGWRFTDYGPHYSS
                     FTDGRMTGGFTTDSAAGAGPLVVIYATDLQGVCAQVAAAGGQITQEIFSFPGGRRFEF
                     RDPSGNRLAVWSE"
     gene            3033..4046
                     /locus_tag="VFF22_20440"
     CDS             3033..4046
                     /locus_tag="VFF22_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5924 family protein"
                     /protein_id="HZX19222.1"
                     /translation="MPAWKQLIFKLIALIQRNPGMVAIFGFASGLASFLLVERQAELA
                     RVLALVMLVSWLWLILENILRERIAQWFGFELPPPLLRYATQMIHQESLFFVLPFFFI
                     TTTWNSGQLLFSGLLASAALISIIDPLYYRWLAPKRWLFLAYHTLALFAVLLTALPII
                     FKLTTPQSYQLALAAAVLLSFPSLFAIITVQRWWRGLLLVGMTLAIGAGGWLARTWVP
                     PATLWLTEVAVTSEFDNQNRAPGESIKELSVAQLRSQGLFAYTAISAPRGLNERIYHV
                     WRRDGGEVERIALDIHGGRKEGYRAWTHKQNFPEDAVGRWQVQVLTEAGQMIGVLRFK
                     VVE"
     gene            complement(4402..5631)
                     /locus_tag="VFF22_20445"
     CDS             complement(4402..5631)
                     /locus_tag="VFF22_20445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009403410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HZX19223.1"
                     /translation="MLNWLWLGFFVVAAIAGLSRWLLGDDPAVFAAMVESLFAMAKLS
                     VEIMVLLFGTLTLWLGLLRIAEKAGLVDALARMLGPLFARLMPEVPRGHPALGLITMN
                     FAANGLGLDNAATPIGLKAMRALQTLNPSSTTASNAQILFLVLNASSLTLLPVTIFMY
                     RAQQGAPDPTLVFLPILLATSASTLVGLLSVALMQRLRLWDPVVLAYLIPGALLLGSF
                     MAVLATMSATALAALSSLAGNLTLFGIIMLFLLLGALKKVAVYEEFVEGAKEGFDVAK
                     SLLPYLVAMLCAIGVLRASGALEFGLDGIRWLVEVVGWDTRFVDALPTALVKPFSGSA
                     ARAMLIETMQTQGVDSFAALVAATIQGSTETTFYVLAVYFGAVGIQRVRHAVGCALLA
                     EFAGVVAAIFVCYWFFG"
     gene            complement(5867..6325)
                     /locus_tag="VFF22_20450"
     CDS             complement(5867..6325)
                     /locus_tag="VFF22_20450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of vertebrate lysozyme family protein"
                     /protein_id="HZX19224.1"
                     /translation="MTIKTLLAALLLGGSAAALAADTDGQFRLNELLASDPAYQESWQ
                     ELVEDESRLPEWVMNLSGIASPMQGLEDDGDKYLVGQLCEENNCFNQRLYVAFSWDKD
                     DAYALYVQLPDGLPTDKAPSQHATLRWLGEPDEATQQLLQEQLKSDPNWY"
     gene            complement(6336..6500)
                     /locus_tag="VFF22_20455"
     CDS             complement(6336..6500)
                     /locus_tag="VFF22_20455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HZX19225.1"
                     /translation="MLSWAITFLIIAIIAAVLGFGGIAGTATGIAKILFVVFLVMFIV
                     SFIMGRRPRG"
     gene            complement(6578..7012)
                     /locus_tag="VFF22_20460"
     CDS             complement(6578..7012)
                     /locus_tag="VFF22_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HZX19226.1"
                     /translation="MTDKTEQLNELIEITRDGQRFYQHAHDEVKDVRLQVLFRDMSQS
                     KSELIRALSVKVAANQEIPASGGTFVGKLRQVYADTKASLVSDTEATYVAQLEEAEDR
                     ILHAFEDALESAAPDVQALLTAEMPKVRANHDRMRTLKQSMQ"
     gene            complement(7096..7932)
                     /locus_tag="VFF22_20465"
     CDS             complement(7096..7932)
                     /locus_tag="VFF22_20465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZX19227.1"
                     /translation="MHQLKTLALATATAGLIGLAPFAAYAAEGDASRELTEARQEGSI
                     WTAFALNRHLNPFTIDVDVENGSAKLTGKVESDVERDLAEQIALGIEGVKKVDNQLTL
                     DPNFEAKTSAEPSMSQRFDDATLVATVKSKLLWNSNTEGLDINVNAKNGTVSLTGSAQ
                     TAEAKELAGRLAANTDGVRSVDNQLSVSAGDTAAGKAQNAADDTAAVISDAWITSKVK
                     SSFLYSRNLDGLDISVDTKKGMVSLSGSVLSNAEKQLAVETARNIRGVRGVNADALRV
                     GS"
     gene            8185..9537
                     /gene="algB"
                     /locus_tag="VFF22_20470"
     CDS             8185..9537
                     /gene="algB"
                     /locus_tag="VFF22_20470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor AlgB"
                     /protein_id="HZX19228.1"
                     /translation="MHASADEHSLGRILLVDDETTILRTFRYCLEDQGYQVSTASSAE
                     QADALLLRQVFDLCFLDLRLGADDGLDVLAQMRLQAPWMRVVIVTAHSAVDTAVDAIQ
                     AGAADYLVKPCSPEQLRLAAAKQLEARQLVERLERLEGYVRKPGDGLDSQSPALMAVL
                     QTAKQVADTDANILILGESGTGKGELAQAIHRWSKRAKKSCITINCPSLSAELMESEL
                     FGHNRGAFTGASESTLGRVSQADGGTLFLDEIGDFPLALQPKLLRFIQDKEYERIGDP
                     VTRQADVRILAATNLNLADMVQDGRFREDLLYRLNVITLTLPPLRERREDILDLAERF
                     LARFVVDYARPARNFSEEAREALLTYAWPGNIRELRNVIERASIICSEELVQVAHLGL
                     SAQANHCAPQVGDQLSLEALEKAHISAVMANSESLDQAAKTLGIDASTLYRKRKQLSL
                     "
     gene            9534..11309
                     /locus_tag="VFF22_20475"
     CDS             9534..11309
                     /locus_tag="VFF22_20475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017477985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZX19229.1"
                     /translation="MKLRNRLFLSSSALLTVALIGLLLGTFSVLQLTQVQSQAMTRNM
                     QIIDASLGLRQEMGKQVTLLLAEELDREALKVSDQQFKASLQRATESATDDSDRQAIA
                     EIERAYANFDDLLAKPLTARRELLRNDSFSRAFEALRDRINAAQTRYVDAATQEEARL
                     RERAWLIAGLLGMIGLALLVIGFVTAHSIAQRFGKPIEELARAADQIGQGDFQVTLPV
                     TPVVEMSALSQRFGLMAEALREFKNSDVAALQAGQQRLQAVLDSIDDGLLIFDCAGRI
                     EHINPVAQRQLNWGDEQLGQTLDVALNRADLAEQLHRVLQGESLDETLDDVQIQIDGE
                     TRLLAYSLSGVNHHEGHLMCAVMVLRDVTEERAFERVRNEFVLRASHELRTPVTGMLM
                     AFSLLQERMQFTAASREADLLNTVDEEMQRLLRLINDLLNFSRYQNGLQTLELSVCAL
                     EPLLRQTQQRFSAQAAELQISLACEVHGELPDVQIDALQIERVLDNLIGNALRHSHPG
                     GQIRLQARQQDDRVVLSVEDSGEGIPYHQQTRIFEPFVQVSRKKGGAGLGLALCREIV
                     QLHGGQIGVHSRAGQGAQFYVALPV"
     gene            11404..11808
                     /locus_tag="VFF22_20480"
     CDS             11404..11808
                     /locus_tag="VFF22_20480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HZX19230.1"
                     /translation="MPSINPSPAQLAAFTADVADDTPLLMLNLLRFQPQASYASDSSH
                     APCSGREAYARYSQTALRKVRGVGGEVQVMAAAQAALIAPADERWDELLLVRYPSKAA
                     FLSMLADPEYRAATEHRTAALADSRLIATTPR"
     gene            complement(11829..13313)
                     /locus_tag="VFF22_20485"
     CDS             complement(11829..13313)
                     /locus_tag="VFF22_20485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010176247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HZX19231.1"
                     /translation="MTTSPVTPSSAQRPEDENLGIGANLAYGLQHVLTMYGGIVAVPL
                     IVGQAAGLSPADIGLLIAASLFAGGLATLLQTLGLPFFGSQLPLVQGVSFAGVATMIA
                     IIGSDGAGGLPAVFGAVIAASIIGLLITPVFSRITKFFPPLVNGIVITIIGLTLMPVA
                     ARWAMGGNSAAADFGSMANIGLAALTLVTVLLLSKLGSSSISRLSILLAMIIGTLVAV
                     ALGMADFSKVAEGPMVALPSPFHFGMPTFHVAAIISMLIVIIVTLVETSADILAVGDI
                     IETKVDSKRLGNGLRADMISSVFAPIFGSFTQSAFAQNVGLVAVTGVKSRYVVASAGL
                     ILVTLGLLPVMGRLVAAVPTAVLGGAGVVLFGTVAASGIRTLSKVDYRNNMNLIIVAT
                     SIGFGMIPIAAPSFYHHFPAWFETIFHSGISSAAIMAILLNLAFNHFKTGNSDQQSVF
                     VAASDRTLRYQDIAALNDGDYFKGGKLFDVDGKEVPLIKEASGH"
     gene            complement(13523..14299)
                     /locus_tag="VFF22_20490"
     CDS             complement(13523..14299)
                     /locus_tag="VFF22_20490"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZX19232.1"
                     /translation="MKVFCLSFLLLMLAGCSSGLRIDDSHTATGQNSRVQYIVLHYTS
                     ADMARSLQLLTETEVSSHYLIGEAPATIYRLVDENRRAWHAGDSQWQGRTWLNGTSIG
                     IELVNQGFYDTPNGRYWQPFAPEQIDALILLLKDIMQRHQLAPGSILAHSDVAPQRKV
                     DPGPLFPWKRLAEAGLMPWPDAAAVAREQALFSASLPSVQWFQEQLARQGYVVPNHGE
                     LDQATRNVIAALQMKYRPARYDGEPDAETAALLMVLNSMR"
     gene            complement(14296..14889)
                     /locus_tag="VFF22_20495"
     CDS             complement(14296..14889)
                     /locus_tag="VFF22_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HZX19233.1"
                     /translation="MDIFGIAALIFLVTDPFGNIAIFIAALKNVPAKRRLWVAARELV
                     FALALLLLFLTFGDKVLSALGLSREATAIAGGIILFVIAMRLIFPGPQGVLGDVPDGE
                     PMLVPLATPAVAGPSALAVLMTLRNTHDGPLWELYAAVLLAWAATAFILLQASLLQRF
                     LGPRGLTAVERLMGMLLIMLSVDMLLDNLQSVLHISP"
     gene            15071..16501
                     /locus_tag="VFF22_20500"
     CDS             15071..16501
                     /locus_tag="VFF22_20500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003913 - DNA photolyase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASH family cryptochrome"
                     /protein_id="HZX19234.1"
                     /translation="MRALLWFKQDLRLDDHPALQAGLTAECLLPVYVLDPALLQLNEF
                     GLRRMGVHRARFLLESLVALEGELQQRGSHLLVVIGSAEQAIPQLVTQLSLDQVLTLE
                     EIAPDERAQVARVAQRLGDIPLQQAPANNLLRPDELPFSIEQLPPVFSRFRSLVEERL
                     QVFQPRHAPDRLPGLPDGALALMQPLPTLFQLGLGEPLSVPASAFPFSGGEPAALGRL
                     RDYLWKTQSVRQYKDTRNGMIGSEYSSKFSPWLANGSLSARRVVAELRRHEAQFKRND
                     STHWLWVEMLWRDFFRWTLIRHGSALFKAGGLKATERAPQQLDQRFQDWSRGRTGMPL
                     VDANMRELAATGFMSNRGRQVVASYLINDLQQDWRHGAAWFEEHLIDYDPASNWGNWA
                     YLAGVAAGVGNDPRHNRVFNTLRQAREYDPDAQYVSLWLPELRDVPQALRHTPFLLAG
                     GHLRSLGYPQLESIPESWKPYLHAVA"
     gene            complement(16508..18379)
                     /locus_tag="VFF22_20505"
     CDS             complement(16508..18379)
                     /locus_tag="VFF22_20505"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZX19235.1"
                     /translation="MTDDTQRWKSKYLESLEQQEKLERSWAARLDLLRRGLVRSSLAA
                     EGSDKSVDQCMQELRDILRRDDIDAGLSALIPRLEKAVLDSEKRRQDRVEQNISGVTS
                     LTDQLLALEPPREVRKALKQFAKRIEERASQPRELPALLSELSSLQQKALAALEAEPN
                     QARPGFLQRLFGGKEAAPVNPVGSDLALAEIVPPGASAPAVIAQAETAVQAADTSLNL
                     HSEAPKPVLAAVATAGAEPESQTAVAEVLSAAAAPALVALTQDSGDPAYALPEIPEPG
                     YSAVAPHIGDSLMGLLDELELPERHQPQGESLRQRVQGGLNWYELVAVLDDLAVLVLA
                     VTDSGQREFATYLKQLNERLASFLTTLSEAHEGYSESVESARTFNQELREQVSGLQAS
                     VQDAVDLPTLKQSLEARLDGLLSTVSEHQKHRDGHEEEVARRLQSLTQKVADMEQEAQ
                     SFRDHLEEQRQKAMIDPLTGLPNRAGWSERLDLEMARWKRYGGELLLAVLDIDHFKRI
                     NDSYGHLAGDKVLKIIASELAKRLRKTDFIARFGGEEFVLLIPSTPMEGGHQLLQTLL
                     DGVEQCPFHFRGERVTITLSAGLTAFAGEEGSDKVFERADQALYRAKSGGRNRVELS"
     gene            complement(18395..19258)
                     /locus_tag="VFF22_20510"
     CDS             complement(18395..19258)
                     /locus_tag="VFF22_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003282849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZX19236.1"
                     /translation="MLRRWPARRVIDLCDPQVNPRCAPAADWPQNGSLRLLSFNIQVG
                     ISTQRYHHYVTRSWQHLLPHAGRSGNLQRIGDLLGDFDLVALQEVDGGSLRSGYVNQV
                     EHLAHLGAFPYWYQQLNRNLGRLAQHSNGVLSRLRPSLLEDHPLPGPPGRGAMLLRLG
                     EGPDAVAVVMMHLSLGARTRTRQLAYIRELLSDYQHMVLMGDMNTHANDLLLHSPLRD
                     LGLLAPQIEATFPSWRPQRCLDHILLSPGLPLERCEVLSQPISDHLPVAVQIRLPTSL
                     YGPATPMLIEP"
     gene            complement(19282..19911)
                     /locus_tag="VFF22_20515"
     CDS             complement(19282..19911)
                     /locus_tag="VFF22_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HZX19237.1"
                     /translation="MRNLILSATLVTASLFGLSAHAEPIEAGKQYVELNNPVAVAKPG
                     KIEVVELFWYGCGHCYKFEETINPWIDTLPEDVNFVRIPAMFGGMWNVHGQLFITLEA
                     MKAEHKVHAAVFDAIHVQGKKLATPEEMASFLAAEGIDKDAFLKTYNSFGVKSQLEKA
                     KKLAMAYQITGVPVMIVNGKYRFDISSSGGPQQTLQVADHLIAKERAAQ"
     gene            complement(20065..20670)
                     /locus_tag="VFF22_20520"
     CDS             complement(20065..20670)
                     /locus_tag="VFF22_20520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZX19238.1"
                     /translation="MNKVLVSLLLTLGITGLAHAAGNAEAGQGKVVVCGACHAADGNS
                     AAPNFPKLAGQGERYLLKQLNDIKSGARPVVEMTGLLDNLSDQDLEDISAYFASQQMS
                     VGAADPKLVERGGELFRGGKLAEGMPACTGCHSPNGAGLDAAGYPQLGGQHATYIAKQ
                     LTDFREGNRTNDGDSMIMRSIAAKLSNKDIEAVSSFIQGLH"
     gene            complement(20717..21007)
                     /locus_tag="VFF22_20525"
     CDS             complement(20717..21007)
                     /locus_tag="VFF22_20525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZX19239.1"
                     /translation="MKKLLIAASALMLSLSAQAAQDPEAVYARACGVCHSGAIQTAPK
                     RGDKAAWEPRLAKGMDALVLSVTNGLNAMPPRGLCMDCSAEDYQAVIKLMTE"
     gene            21254..21907
                     /gene="yihA"
                     /locus_tag="VFF22_20530"
     CDS             21254..21907
                     /gene="yihA"
                     /locus_tag="VFF22_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HZX19240.1"
                     /translation="MQAKNPIIGLCQQATFLISAAKVDQCPEDFGHEVAFAGRSNAGK
                     SSALNTLTHASLARTSKTPGRTQLLNFFKLDEERRLVDLPGYGYAKVPIPLKLHWQKH
                     LEAYLGSRECLRGLMLMMDIRKPLTEFDILMLDWSVASEMPMHILLTKADKLAFGAAK
                     NTLLKVRQDIHQRWGDRVSIQLFSAPKRIGIEDAQEVLARWMQLGEFALPEDEEVDA"
     gene            complement(22202..25057)
                     /gene="polA"
                     /locus_tag="VFF22_20535"
     CDS             complement(22202..25057)
                     /gene="polA"
                     /locus_tag="VFF22_20535"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HZX19241.1"
                     /translation="MSQAPLVLVDGSSYLYRAFHALPPLTTSKGLPTGAVKGVLNMLK
                     SLRKQYPKSPFAVVFDAKGGTFRDEMFAEYKANRPSMPDELRVQVEPLHASVRAMGYP
                     LLCVEGVEADDVIGTLARQCAAEGRDVVISTGDKDMAQLVCPHVTLVNTMTGSVYDID
                     GVKAKFGVGPELIIDYLALMGDKVDNIPGVPGVGEKTALGLLVGVGGGLDVLYANLDK
                     VPELPIRGAKTLPAKLTEHKDMAYLSYALATIKLDVPLNIAIDSLHPSEMDVPALVEL
                     YTELEFKTWRDELLRSNPAPVVKTPVAAPADDLFALAPSASAAAVVAPAPAAADAPTA
                     DAVVSELPAATALGDYQCVLTQAQFDEWLEKLRNAELIAFDSETTGLDAQKAQLVGLS
                     FAVKAGEAAYIPVAHSYMGVPTQLDRDVVLQALKPILEDPKKAKVGQHAKYDINILAN
                     ASTPIRVQGVAFDTMLESYVLDSTATRHDMDSLALKYLDHSNIRFEDIAGKGAKQLTF
                     DQIDLAQAAPYAAEDADVTLRLHQHLWAKLSAESSLAKVLQEIEMPLVPVLARIERQG
                     ALVDAKLLGLQSNELGEKLVALERQAFEIAGEEFNLASPKQLGVILYEKLGLPVISKT
                     AKGQASTAEAVLAELAEQDFELPKVLMQYRTLSKLKSTYTDRLPEQINPRTGRIHTSY
                     HQAVAATGRLSSTDPNLQNIPIRTAEGRRIRQAFVAPKGYKLLAADYSQIELRIMAHL
                     AKDESLLDAFRHGRDVHKATAAEVFGVPLDQVTGDQRRSAKAINFGLIYGMSAFGLAK
                     QIGCDRKQAQAYIDVYFARYPGVLAYMERTREQASEQGFVETLFGRRLYLPDIHAKNQ
                     ALRKGAERTAINAPMQGTAADIIKRAMVTVDNWLSESGLDAKVILQVHDELVLEVRED
                     LVEQVSAQIRPLMGNAAALDVPLLVEVGVGDNWDEAH"
     gene            25276..25560
                     /locus_tag="VFF22_20540"
     CDS             25276..25560
                     /locus_tag="VFF22_20540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HZX19242.1"
                     /translation="MRALNRLLLASLLAFTTLVAHAEDPVSADPDVTIRQDGDRTIHE
                     YRVNGFLYAVKVIPKGGKPYFLVRADGSDGNFVRSDSPDMLIPAWEIFSW"
     gene            25576..26526
                     /locus_tag="VFF22_20545"
     CDS             25576..26526
                     /locus_tag="VFF22_20545"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HZX19243.1"
                     /translation="MSVFTPLERHELEAFLAPYDLGRLRDFQGIAAGSENSNFFVSLE
                     QGEFVLTLIERGPSADLPFFIELLDVLHDAGLPVPYALRTTNGEALRSLAEKPALLQP
                     RLAGKHVHDANPHHCQEVGALLARIHLATRNQPLPRKSDRGLDWMLAEGPSLALQLPD
                     EQLPLLRDALAEIHALKPRILALPQANLHADLFRDNVLFDGNHLAGVIDFYNACSGPM
                     LYDLAITLNDWCSCEDGSLDSKRAQALLGAYAGLRAFTAAEAELWPAMLRIACVRFWL
                     SRLIAAESFAGQEVLIHDPAEFQRRLAQRQQVELALPFAF"
     gene            complement(26540..>27083)
                     /locus_tag="VFF22_20550"
     CDS             complement(26540..>27083)
                     /locus_tag="VFF22_20550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016394240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HZX19244.1"
                     /translation="HDHAHRPGSLDAHLWLLPANARVIAGKIATDLAQADPANAARYQ
                     ANAKAFGERLDALDQRLKTRLSSLAGKPYFVFHEAYDYFEAAYGLKHAGVFSVLGEVQ
                     PGAQHVAAMRERLQQAGPSCVFSEPPFRPRLAQTLTAGLPVTLAELDAMGGALPVDAK
                     GYEALLENLAGGLAGCLEAL"
BASE COUNT         5322 a         8422 c         8234 g         5105 t
ORIGIN      
        1 tttgaccacc ggtgccgatg gtgacgaatt tctcttcggc ctgtacggcg ctgctcaggg
       61 taatacctgc gagtgcagcc gacagggccc atgccaggga tttacctttc atgggagttc
      121 tccttttctg tttatttttg aaccggtcgc accttttgag tgcgtccgga ggacaagagt
      181 ctaaccgctc agccacaggt tgcacggagt aataccggcg cccctggtaa ccaagtgtta
      241 gagagagtct gttgccgttt cgttcgcggc ggcgcgaatg gaacagagtc tagacgtctt
      301 aacgctacct tcaggccaca gctttgggca gaatcaggcc atgcccaagc acacactcga
      361 ctcgcaacac acctcagtca gcgccagcat caccctcgcg gtgctgcatg ccgccagtcg
      421 cctgggcgtt gatcgcgccc aattgatgag cgcctgcgag ctgcacgaca gccagctcag
      481 cgaccccgat gcgcgcgtac ccttcgctac tcaggaacaa ctctggcaac agcttagccg
      541 catcgagcat gccgaacccg gtctggctct gggctgcaac ctcgcccctg gcccgttcag
      601 cgtgctcggt tacctgttgc agagtagccc gaccctgggc gacgcgctgg ccgccgggct
      661 gcgctatcaa cgcctggtgg gcgagggcgg cgagttgcaa ttacgcgaac agggcgatga
      721 gctgcagctg ctctaccgcc cgttgcatcc cgaactaccg gccacccgca cccgcgtact
      781 ggcgctgatg gcctgttggg tgcagatgct gcatccgctg ctggataact ggcagctgtt
      841 gcgcgcgcag ttcgctcacg aacagccacc gacgctggac agttacagcg aaaccttcgc
      901 ctgcccactg cagtttgccg ccagcgatta cggcatcgta ctgccgcgca gcctggccca
      961 cgcgccgctg attcaggcca acccaccgct gcagaacctg ctacgccagc atgccgaagc
     1021 cctgctcgcc cgcctgccca gtgaagggtt aagcgcccgc gtggtggctc tgctcggcga
     1081 gcaactggcg cgcggcgagc cggaccgcag cgaactggcg cgccatctgc acctgagcga
     1141 acgcaccctg caacggcgcc tggccgacga aggctgcaac taccagcaac tgcttaacga
     1201 cacccgccag cagctggccg aacaacacct cagcaccggc gacttacccg ccgcagaaat
     1261 cgccctgctg ctgggctact cggaacccag cgtatttttt cgcgcgtttc gccaatggac
     1321 tgggttaaca ccgggtgaat atcgggcgcg gcagaaagct caatgaccgc aggctaaatc
     1381 ccctgcgccc gcaaaaaagc caaaaacgcc tcgcgcgagc tatagcgtgg gctaaagccg
     1441 aaatcatgtt taagccggcg attgctcagc accgggcgat aacgcagaaa atccacttgt
     1501 tccgggccgt aggggcttag gcccaggggt ttgagtacgc tcagtgcagc gcgaatcagc
     1561 caggccggta aggcgcggta gggtttgtta agcagctcgg cgatttcctt gagcgatagc
     1621 gcgccatcac cggccaggtt gaagatgccg gcgctttgtt ggcgcaggcc acggcagatg
     1681 atgtccacca catcctgatc ccagataaat acaaagcggc tgtcgctgcc gcggatgccc
     1741 agcaccgccg gtttcttgaa cagctcggtg atctggttgt ttacctgttt gccgagaatg
     1801 gtgccggggc gcagcaccag ctgcttgagc tgcggatgct gcacgcgcgc ctcggcgagc
     1861 agcaactcca cttcacgttt gtgcttggcg taggcgaaat gggcgtggcc gcgtagcggc
     1921 tgatcctcat ccagccactc ggcattctgc gggtcatagc catacgcggc gcccgagctg
     1981 gtgacgatca gctgttcgac gccatgggcc gcagccgcct ggataagcgc ctgggtgccg
     2041 cccacatcga tggcatgcag ctgcgcctcg ctcatacccg gcggtggcgt gaccaccgag
     2101 gccaggtgga caatcgcctg cggctgccag gtggctacgc aatcttccac ggccgtcggc
     2161 tggctgatat caagcagcac cggttcgata ttcagcttta tccccgcgcg gcactgcggg
     2221 cggatatccg cggcaatcag cagccactgt ggctgctgaa cggccagttg gttaagcaac
     2281 tgctggccga tatagccggc agcccccgtg atcaagacac gcatttaact ctccatacgc
     2341 agcgaacccg tggcgcgggg cgctacgttt tcgccgactg tgtttaccca ttgcaggcta
     2401 tccagcggat tgcggagagg cagtggcaag gctggccaaa cagcattgcc aaaggcgaca
     2461 gatgccctgt gccctggccg actgagcgcg ctatgctctg cgcccgccat tcgcccaggt
     2521 atgcccgcca tgagccaagc cgaacagaat ctgcgtatcg actacatcga gttcgccgcc
     2581 gtcgacctgc cggcgctcaa gcagttctat gaacaggtct tcggctggcg ctttaccgac
     2641 tatggcccgc actacagcag ctttaccgac gggcgcatga caggcggctt taccaccgac
     2701 agcgcagcgg gtgccgggcc gctagtggtg atttacgcca ctgatctgca gggcgtttgc
     2761 gcccaggtgg cggctgcggg cgggcagatc actcaggaaa ttttcagctt ccccggtggg
     2821 cgacgcttcg agtttcgcga tccgagtggt aaccgcctgg ccgtctggtc ggaataacaa
     2881 cctgtccaaa gtctgctgcg cgtcggccct gctgcgttga aaacaggctc ggaatgctca
     2941 tttacaccag taaactccgc ttcctcgcct gttttcgcct tgcatcgctc tagctcgcga
     3001 gactttgcac aggttctaat cgagtccaat ttatgcctgc gtggaaacaa ctgatcttca
     3061 agctgattgc gctgattcag cgcaatcccg ggatggtggc gatcttcggt tttgcctcgg
     3121 ggctggcgag ctttctgctg gtcgaacgcc aggccgagct ggcgcgggtg ttggcgctgg
     3181 tgatgctggt cagctggttg tggctgattc tggaaaacat cctgcgcgaa cgtattgccc
     3241 agtggttcgg cttcgagttg ccgccgccgc tgctgcgcta tgccacgcag atgatccacc
     3301 aggagagtct gttcttcgtc ctcccgttct tctttatcac caccacctgg aacagcggtc
     3361 agctgctgtt cagcggtttg ctggccagcg cggcgctgat ctcgattatc gacccgctgt
     3421 actaccgctg gctggcaccg aaacgctggt tgtttcttgc gtatcacacc ctggcgctgt
     3481 tcgccgtgct gctgaccgcg ctgccgatca tcttcaaact gaccaccccg cagagttatc
     3541 agttggcctt ggcggcggcg gtgctgctgt cattcccgtc gctgttcgcg atcatcaccg
     3601 tgcaacgctg gtggcgcggc ctgctgttgg tgggcatgac cctggccatc ggcgcgggcg
     3661 gctggctggc gcgcacctgg gtgccgccgg caaccctgtg gctgaccgag gtggcagtga
     3721 ccagcgagtt cgataaccag aaccgcgcac cgggcgaaag catcaaggaa ttgagcgtgg
     3781 cgcagctgcg cagccagggc ctgttcgcct acaccgcgat cagtgcgccg cgcgggctga
     3841 atgagcggat ttatcacgtc tggcgccgcg atggcggcga agtcgagcgc atcgccctgg
     3901 atatccacgg cggacgcaag gaaggctacc gcgcctggac ccacaaacag aacttccccg
     3961 aagatgccgt gggccgctgg caggtgcagg tgctgaccga agcggggcag atgattggtg
     4021 tgctgcggtt taaggtggtg gagtagcggc ttagcatcgg aggcctatcg gcacgtttgc
     4081 gggatttttc gcaaaactaa agcccgcttt tgctcttgca ccacgttcgt gcagacgacg
     4141 ccaagatccc ctttaggagg ccgagtgaaa tagccgcgta aggggttgag cgacatggat
     4201 gtcgcgagag ccacgatggg ccatggatgg cccttcgtgg cgtgcccctg gagtggtgat
     4261 ggaaggaggg aaccccggcg cagccggggc cggatgatgg ggtgcccttc tctttggtta
     4321 ctttctcttg ggcaagcaag agaaagtgac tcgcccgtaa ggggcgaaac cgcaacacaa
     4381 gggcgacgcg cagtcgaacg cttaaccgaa aaaccagtag caaacgaaga tcgccgccac
     4441 cacgccagca aactcggcca gcagcgcgca gcctacggcg tggcgtacgc gctggatgcc
     4501 gaccgcgccg aagtacaccg ccagcacata gaaggtagtt tccgtgctgc cctggatggt
     4561 cgcggccacc agcgcggcaa agctgtcgac gccctgggtc tgcatggttt cgatcagcat
     4621 ggcgcgcgcc gcgctgccgg agaagggctt gaccagcgcg gtgggcaacg cgtcgacaaa
     4681 gcgcgtatcc cagccaacca cctcgaccag ccagcgaatg ccgtcgaggc cgaactccag
     4741 tgcaccggag gcacgcagta cgccaatcgc gcagagcatc gccaccaggt agggcagcag
     4801 gctcttggcc acgtcaaagc cttctttcgc gccttcgaca aactcctcgt acaccgccac
     4861 cttcttcagc gcgccgagca gcagaaacag catgatgatg ccgaacaggg tgaggttgcc
     4921 ggccagcgat gacagagccg ccagcgcggt ggccgacatg gtggccagca cggccataaa
     4981 actgccgagc agcagcgcgc cggggatcag gtaggccagc accaccgggt cccaaagccg
     5041 caggcgctgc atcagcgcta ccgacagcag accgaccagg gtcgaggcgc tggtggctag
     5101 caggatcggc agaaatacca gagtcgggtc aggcgcgccc tgctgggcgc ggtacataaa
     5161 gatggtcacc ggcagcaggg tcagcgacga ggcgttgagc accagaaaca ggatctgtgc
     5221 attgcttgcc gtggtgctgc tggggttgag ggtctgcagc gcgcgcatgg ctttcaggcc
     5281 aatcggcgtg gcggcgttgt ccaggcctag gccattggcg gcgaagttca tggtgatcag
     5341 gccgagggcc ggatgaccgc gcggcacttc cggcataagc cgcgcaaaca gcgggccgag
     5401 catgcgcgcc agggcatcga ccaggccagc tttttccgcg atacgcagca ggccgagcca
     5461 cagggtcagg gtgccgaaca gcagcaccat gatctcgacc gacagcttgg ccatggcaaa
     5521 caggctttcg accatggcgg cgaacaccgc cgggtcgtcg cccagcaacc agcgtgacag
     5581 gccggcaatt gccgccacca cgaaaaaccc cagccacagc caattgagca tgccgtctct
     5641 cccccagatc aatgggggcg atgatagcgc ggcttataca aaggaggtgg agagagtctg
     5701 ctcgtcgttg ccacgcagag cgtgggagca gtcggtaata ggtccgagca ttgccagttg
     5761 gtgtagaggg gccgggcggc gatccgcttt agccgcgatt gagcttggcg gcgccaaaag
     5821 cgtcgcggct aaagcccctc ccacgacaca gcactcatgc gctgcactag taccaattcg
     5881 gatcgctttt cagctgctct tgcagcaatt gctgagtcgc ttcatccggt tcgccaagcc
     5941 agcgcaaggt ggcgtgctgg ctgggtgcct tgtccgtcgg caggccgtcg ggcagttgca
     6001 catagagggc gtaagcgtcg tccttgtccc agctgaaggc tacgtagagg cgctggttga
     6061 aacagttgtt ttcctcacac agctggccaa ccagatactt gtcaccgtca tcttccagac
     6121 cctgcatagg gctggcgatg ccgctgaggt tcatgaccca ctccggcaga cggctttcat
     6181 cttccaccag ctcctgccag ctttcttgat aagccgggtc actggcgagc agttcattga
     6241 ggcggaattg gccatcggta tccgcggcca aagccgccgc actgccgccc agcaacaggg
     6301 cggcaagcag cgtcttgatg gtcatggcgg ctcctctatc cacgtgggcg gcggcccatg
     6361 atgaaggaaa cgataaacat caccaggaac accacgaaga ggatcttcgc gatacccgtg
     6421 gcggtgccgg cgataccacc gaagcccagt acggcggcga tgatggcgat aatcagaaag
     6481 gtaatggccc aactcagcat ggcgttactc cttgtcactc aggtgtgcgc cgcggcgcgt
     6541 taagtcccgg tgtggcgcac gccacaccgg tccggactta ctgcatggac tgtttcaggg
     6601 tgcgcatacg gtcatggttg gcgcgcactt tcggcatttc tgcggtcagc agggcctgca
     6661 catccggcgc ggcgctttcc agcgcatcct cgaaggcatg caggatgcgg tcttcggctt
     6721 cttccaattg cgccacataa gtagcctcag tgtcgctgac caggctggcc ttggtatcgg
     6781 cgtaaacctg acgcagcttg ccgacgaagg tgccgccgct ggccgggatt tcctgattgg
     6841 cggcgacttt caccgacagc gcgcggatca gctcgctctt gctctgcgac atgtcgcgga
     6901 acagcacctg taggcgcacg tccttcactt catcgtgggc gtgctggtag aagcgctggc
     6961 cgtcacgggt gatttcgatc agttcgttaa gttgctctgt tttgtcagtc atggtggatc
     7021 tcctatgagt tgtagcggct gcaagggcag cctgagacaa caggcagtgg ccggtatggc
     7081 cactgcccgg atggattagc taccgacgcg cagggcatcg gcgttgacgc cgcgcacgcc
     7141 acgaatgttg cgggcggttt caaccgccag ttgcttctcg gcgttgctca gcacgctgcc
     7201 gctgaggctg accatgccct tcttggtgtc gactgagatg tccagaccgt cgaggttgcg
     7261 gctatagaga aagctggatt tcaccttgct ggtgatccac gcatcgctga tcaccgctgc
     7321 ggtgtcatcg gccgcgttct gcgccttgcc tgcagcagtg tcgccagcac tgacgctcag
     7381 ctggttgtcc acactacgca caccatcggt gttggccgcc aaacgacccg ccagttcctt
     7441 ggcttcggcg gtctgcgcgc taccggtcag gctgaccgtg ccatttttcg cattgacgtt
     7501 gatgtccaga ccttcggtgt tgctgttcca cagcagcttg gatttcaccg tagccaccag
     7561 ggtcgcatca tcaaaacgct gcgacatgct cggctcggcg ctggtcttgg cttcgaagtt
     7621 cgggtcgagg gtcagctggt tgtcgacctt cttcacccct tcaataccca gggcgatctg
     7681 ctcggcgaga tcacgctcga catcgctttc caccttaccg gtgagcttgg cgctgccgtt
     7741 ctccacgtct acatcaatgg tgaaggggtt gaggtggcgg ttaagggcaa acgcggtcca
     7801 gatcgagcct tcctggcgcg cctcagtcag ctcgcggctg gcatcgccct cggcagcata
     7861 ggcagcgaaa ggagccaggc cgatcaggcc ggcggtcgca gtggccagag ccagagtttt
     7921 caattgatgc atggcagttc ctccaatgaa taacgatcaa gcaagcagcc gtttgtcgca
     7981 gccgttactg atagatcgca agtgccatgc cagcttaaaa actcaataaa aaaatataaa
     8041 tatcaacaag ttaaacaaat tcaccagcat cgccttacgt gcaccttgca tgatcactgc
     8101 agaacggccg tgcaacttgc acgacgcgcc gcggactaat cctctgcacg cagtacttgt
     8161 gtacggataa ataaggagat ggcgatgcat gcaagcgcag acgaacacag cctggggcga
     8221 attttgttgg tggatgacga gaccaccatc ctgcgcacct tccgctactg cctggaagac
     8281 cagggttacc aggtgagtac cgcgagcagt gcagaacagg ccgacgccct gctgctgcgc
     8341 caggttttcg atctgtgttt tctcgacctg cgtctgggcg ccgacgatgg tctcgacgtg
     8401 ctggcgcaaa tgcgcctgca ggccccctgg atgcgcgtgg tgatcgtcac cgcacactca
     8461 gcagtggata ccgccgtgga tgccatccag gccggcgcag cggactacct agtcaagccg
     8521 tgcagccccg agcagctgcg cctggccgca gccaaacagc tggaagcgcg ccaactggtc
     8581 gagcgcctgg agcgcctgga aggttatgta cgcaaacccg gcgacggcct cgactcgcag
     8641 agcccggcgc tgatggcggt gctgcagacg gccaagcagg tggccgacac cgacgccaac
     8701 atcctgattc tcggcgagtc cggcaccggc aagggtgagc tggcgcaagc cattcaccgc
     8761 tggagcaagc gcgcgaagaa gtcctgcatc accatcaact gcccgtcact cagtgccgag
     8821 ctgatggaaa gcgaactgtt tgggcataac cgcggcgcct ttaccggcgc cagtgaaagc
     8881 accttggggc gggtcagtca ggccgacggc ggcaccttgt ttcttgatga aatcggcgat
     8941 tttccgctgg ccctgcaacc caagctgctg cgctttatcc aggacaagga gtacgagcgc
     9001 atcggtgacc cggtgacgcg tcaggccgat gtgcggattc tcgccgcaac caacctcaat
     9061 ctcgcggaca tggtgcagga cgggcgcttc cgtgaagacc tgctgtatcg cctcaacgtc
     9121 atcaccctga ccctgccgcc gctgcgcgag cgccgcgagg atatcctcga cctggccgaa
     9181 cgctttctgg cgcgcttcgt cgtcgactac gcacgccccg cgcgcaactt cagcgaggag
     9241 gcgcgcgaag cgctgcttac ttacgcctgg ccgggcaata tccgcgagct tcgcaacgtc
     9301 atcgagcgcg ccagcatcat ctgcagcgaa gagctggtgc aggtcgccca cctagggctg
     9361 agcgcccagg ccaaccactg tgcgccgcag gtcggtgatc agttaagcct cgaagccctg
     9421 gaaaaagccc atatcagcgc cgtgatggcc aatagcgaaa gcctcgacca ggcggcgaaa
     9481 accctcggca tcgatgcctc gaccctgtac cgcaaacgca agcagctcag cctgtgaagt
     9541 tgcgcaaccg gctgttcctc agcagcagcg cactgcttac cgtggccctg ataggactgt
     9601 tgctggggac gttcagcgtg ctgcagctga cccaggtaca gagccaagcg atgacccgca
     9661 acatgcagat catcgatgcc agcctgggcc tgcgccagga gatgggcaag caggtgaccc
     9721 tgctgctcgc cgaagagctc gaccgcgaag ccctgaaagt ctccgatcag cagttcaagg
     9781 cctcgctgca acgcgccacg gaaagcgcca cggatgacag tgaccgccag gcgattgccg
     9841 aaatcgagcg cgcctacgcc aacttcgatg acctgctggc caaaccgctg accgcgcgcc
     9901 gcgagctgct gcgcaatgac agcttcagcc gcgccttcga agccctgcgt gaccgcatca
     9961 atgcggcgca gacgcgttat gtcgacgctg cgacgcaaga agaagctcgc ctgcgggagc
    10021 gcgcctggct aattgccggc ctgctgggaa tgatcggcct agcgctgctg gtgattggct
    10081 ttgtcaccgc gcacagcatc gcccagcgtt tcggcaagcc gattgaggaa ctggcgcggg
    10141 ccgccgacca gatcggccag ggggatttcc aggtcaccct gcccgtgacg ccagtggtgg
    10201 aaatgtccgc cctaagccag cgtttcggcc tgatggccga agccctgcgc gagttcaaga
    10261 acagtgatgt ggcagcgttg caggccgggc aacaacgcct gcaggcagta ctcgacagca
    10321 tcgatgacgg cctgctgatc ttcgattgcg ccggccgtat cgagcacatc aacccggtgg
    10381 cccagcgtca gttgaactgg ggcgacgagc agctcgggca gacccttgat gtggccctaa
    10441 accgcgccga cctggccgaa caactgcacc gggtgctgca aggcgaaagc ctggacgaga
    10501 cgctggacga tgtgcagata cagatcgacg gggaaacccg tttgctggcc tacagcctgt
    10561 ccggggtcaa tcaccatgaa ggccacctga tgtgcgcggt gatggtgcta cgcgatgtca
    10621 ccgaggagcg cgctttcgaa cgggtgcgca acgagttcgt gttgcgcgcc tcgcacgagc
    10681 tgcgcacccc ggtgaccggc atgctgatgg cgttctcgct gctgcaggag cgcatgcagt
    10741 tcaccgccgc ctcccgcgaa gccgatctgc tgaataccgt ggatgaggaa atgcagcgcc
    10801 tgctgcgcct gatcaacgac ctgctgaatt tctcgcgcta ccagaacggc ctgcaaaccc
    10861 tggagctgtc cgtctgcgcc ctggagccgc tgctgcggca aacccagcag cgcttcagcg
    10921 cccaggccgc cgagctgcag atcagcctgg cctgcgaggt acacggcgag ctgccggacg
    10981 tgcagatcga tgcgttacag atcgaacggg tgctcgacaa cctgatcggc aacgccctgc
    11041 gccacagcca ccccggcggg cagatccgcc tgcaggcgcg gcagcaggat gatcgggtgg
    11101 tactcagcgt ggaagacagc ggcgagggca ttccctatca ccagcagacg cggatcttcg
    11161 aacccttcgt ccaggtcagc cgcaagaaag gcggtgcagg cctgggcctg gcgctgtgca
    11221 gagagatcgt ccagctgcat ggcgggcaga tcggtgtgca ttcgcgcgcc ggtcaaggtg
    11281 cacagtttta tgtcgcgctg ccggtttaac gcgtcgatta cgcacaatca gcccggctaa
    11341 tgcagattgc ctgttggccg gtgcctgtcg acactgggcg ctccactccc gaggagcgcc
    11401 gccatgccca gcatcaaccc cagcccagcc caactggccg cattcaccgc cgatgtggcg
    11461 gacgatacgc cgctgctgat gctcaacctg ctgcgctttc agccccaggc cagctatgcc
    11521 agcgacagca gtcatgcacc gtgcagcggc cgcgaggcct atgcgcgcta cagccagacg
    11581 gcgctgcgca aggtgcgtgg ggtcggcggc gaggtgcagg ttatggccgc cgcgcaagcc
    11641 gcgctgattg ccccggccga tgaacgctgg gatgaactgc tgttggtgcg ctacccatcc
    11701 aaggcggcct ttctcagcat gctcgccgac ccggagtacc gcgcggcaac tgaacaccgc
    11761 acagccgccc tggccgactc acgcctgatc gccaccacgc cgcgctaaag cgcgtgtggc
    11821 aggctcagct agtgccctga agcttccttg atcagcggta cttccttgcc atccacgtca
    11881 aacagcttgc cacccttgaa gtagtcgccg tcgttcagag cggcaatgtc ctgatagcgc
    11941 agggtacggt cgctggcggc gacaaatacc gattgctggt ccgagttgcc ggtcttgaag
    12001 tggttaaacg ccaggttgag caggatcgcc atgatcgccg ccgagctgat gcccgagtgg
    12061 aaaatggttt cgaaccaggc cgggaagtga tgatagaagc tcggcgcggc aatcgggatc
    12121 atgccgaaac cgattgaggt ggcgacgatg atcaggttca tgttgttgcg ataatcgacc
    12181 ttggacagcg tgcgaatgcc gctggccgcc acggtgccga acaacaccac accggcacca
    12241 cccagtaccg ccgtgggcac ggcggcgacc agacgaccca tcaccggcag caggccgagg
    12301 gtcaccagaa tcaacccggc cgaggccacc acatagcggc ttttcacccc ggttacagcc
    12361 accaggccga cgttctgggc aaaggcgctt tgggtgaagg agccaaatat cggcgcaaat
    12421 acgctggaga tcatgtccgc acgcaggcca ttgcccaggc gcttggagtc gaccttggtt
    12481 tcgatgatgt caccgacggc gaggatgtcc gccgaggttt ccaccagggt gacgatgatc
    12541 acgatcagca tcgaaatgat ggccgcaaca tgaaaggtcg gcatgccgaa atggaacggg
    12601 ctgggcagcg ccaccatcgg tccctcagcc accttggaaa aatccgccat gcctagggcc
    12661 acggccacca gagtgccgat aatcatggcc aacaggatcg acaggcgcga gatgctcgaa
    12721 ctgcccagct tgctcaatag cagcacagtc accagggtca acgccgccag gccgatattg
    12781 gccatgctgc caaaatccgc tgctgcactg ttgccaccca tagcccagcg cgccgccacc
    12841 ggcatcaggg tcaggccgat gatggtgatg acaatgccat tgaccagcgg tggaaaaaac
    12901 ttggtaatcc gtgaaaacac cggggttatc aacagtccga taattgaggc ggcaatcacc
    12961 gcaccgaaca ccgcagggag accgccagca ccatcgctgc cgatgattgc aatcatggtt
    13021 gccacgcccg caaaggacac gccctgtacc agcggcaatt gcgagccgaa gaacggcaaa
    13081 ccgagggttt gcaacagggt tgccaggccg ccggcaaaca gcgaggccgc aatcagcagg
    13141 ccgatatcgg caggcgacag gccggccgcc tggccaacga tcaagggcac cgcaacgatg
    13201 ccgccataca tggtcagtac gtgctgcagg ccgtaggcga ggttggcacc gataccaagg
    13261 ttttcgtcct ccggacgctg cgcggaggac ggcgtaacag gtgaagtcgt catgctgagg
    13321 tactcgctgt ttgttcttgt gcgagcaatg tagacaaaag aaaacagtcc tggctacaac
    13381 tttgtataca ttttcataaa aaagagtgcc gccagaacga ttgctgaatg ctcagtctac
    13441 aaacgaacac ggacacataa aaaaccgccg aaacagactc tgtcccggcg gtttgtttat
    13501 ccgcgaccct ctacatcggg cttcagcgca tgctgttcaa taccatcagc agcgccgccg
    13561 tctcggcatc cggctcgccg tcatagcgcg ccgggcggta tttcatctgc aaggcggcga
    13621 tcacgttgcg tgtggcctgg tccagttcgc catggttggg cacgacgtac ccctggcgcg
    13681 ccaactgctc ctgaaaccac tgcacgctcg gcaggctggc gctgaacagc gcctgctcgc
    13741 gcgccactgc ggcggcatcc ggccagggca tcagaccggc ctcagccaga cgcttccagg
    13801 gaaacagcgg gccggggtca accttgcgct gcggcgcgac atcgctgtgc gccagaatgc
    13861 tgccaggcgc cagctggtgg cgctgcatga tgtccttgag cagcaggatc agcgcatcga
    13921 tctgctccgg ggcaaagggc tgccagtagc ggccatttgg ggtgtcatag aaaccctggt
    13981 tgaccagctc gataccaatc gaagtgccat tgagccaggt gcggccctgc cattggctgt
    14041 cgccggcatg ccaggcgcgg cggttttcat cgaccaggcg gtagatggtc gcgggcgctt
    14101 cgccgatcag gtaatggctg ctgacttcgg tctcggtcag cagttgcagc gatctcgcca
    14161 tatcggcaga ggtgtagtgc agcacgatgt actgcacgcg gctgttctgc ccagtggcgg
    14221 tatggctgtc atcgatgcgc aggccgctgc tgcaaccggc cagcattagt agcaaaaaac
    14281 tcaggcaaaa aactttcatg ggctgatatg caacacgctt tgcaggttat ccagcagcat
    14341 gtcgacactg agcataatca acaacatgcc catcagccgc tccactgcgg tcaacccgcg
    14401 aggtccgaga aaacgctgca gcaacgaggc ctgcaacaaa ataaatgcgg tcgccgccca
    14461 ggccagcaac accgcggcgt agagctccca gagcggcccg tcgtgggtat tgcgcagggt
    14521 catcaacacc gccagagcgg aaggcccggc caccgctggg gtggccaacg gcaccagcat
    14581 cggttcgcca tccggcacat cacccaacac gccttgcggg ccgggaaaga tcaggcgcat
    14641 ggcgatcacg aacaggatga taccgccggc aatcgcggtt gcctcccgcg acaggcccag
    14701 ggcgctgagc actttgtcac caaaggtaag aaacagcagc aacagggcca gggcgaacac
    14761 cagttcacgc gcggccaccc acaggcgacg cttggccggc acgttcttca gcgcggcgat
    14821 aaagatggcg atattgccga aggggtcggt gaccagaaag atcaacgcgg cgatgccgaa
    14881 aatatccata gctactcctc aacagatcgg cagagtttag tgtctatcga gatagaaaac
    14941 gcctttgagc tggcagaaac gccacgccgg tctgttgcct gggcaaggtg tgactggcgc
    15001 tgctagacta gaaatctgta caaatatgga ttttttgtat agttcttgta tcaaccagga
    15061 gatcagcaca atgcgcgcac tgctgtggtt caaacaggat ctacgccttg atgaccaccc
    15121 cgccctgcag gcgggtctga ccgccgaatg cttgctaccg gtgtatgtac tggacccggc
    15181 actgctccag ctcaacgaat ttggcctacg ccgcatgggc gtacaccgcg cgcgcttcct
    15241 actggaaagc ctggtggcgc tggaaggtga attgcagcag cgcggctcgc acctgctggt
    15301 ggtgatcggc tcagcggaac aggccattcc tcagctggtc acccagctca gcctggacca
    15361 ggtgctgacc ctggaggaga tcgcccccga cgagcgcgcc caggtggccc gtgtagccca
    15421 gcgtctcggt gacattcccc tgcaacaggc gccagccaac aacctgctgc gcccagatga
    15481 actgcccttc agcatcgagc aactgccacc tgtattcagc cgcttccgca gcctggtgga
    15541 agaacgcctg caagtgttcc agccgcgtca tgcccccgat cgcttacccg gtttgccgga
    15601 tggcgcgctg gcgctgatgc aaccactgcc gacactgttc caattgggcc tgggtgagcc
    15661 gcttagcgta ccggccagtg cctttccgtt ctccggcggc gaacctgcgg cgctgggccg
    15721 gctgcgtgac tatctatgga aaacccagag cgtgcggcag tacaaggaca cccgcaacgg
    15781 catgatcggc agcgaatatt cgtcaaaatt ctcgccctgg ctggccaacg gcagcctgtc
    15841 cgcacgccgc gtggtggccg agctgcgccg gcatgaggcg caattcaaac gcaatgactc
    15901 gacccactgg ctgtgggtgg aaatgctttg gcgcgatttc ttccgctgga ccctgattcg
    15961 ccacggcagt gcgctgttca aggccggcgg cctgaaggcc accgaacgcg cgccgcagca
    16021 gttggatcaa cgctttcagg actggtcacg cgggcgcacc ggcatgccgc tggtggatgc
    16081 caatatgcgc gaactggccg ccaccggttt tatgtccaac cgcggcaggc aggtggtcgc
    16141 cagctacctg atcaatgacc tacaacagga ctggcgccac ggcgcggcct ggttcgagga
    16201 acacctgatc gattacgacc cggccagcaa ctggggcaac tgggcctact tggcaggcgt
    16261 ggcggccggc gtgggcaacg acccacggca caaccgggtg ttcaatacct tgcgccaggc
    16321 ccgtgaatac gaccccgacg cgcagtacgt cagcctctgg ctaccggagc tgcgcgatgt
    16381 gccgcaggcg ctgcggcata ccccgtttct gctcgccggc ggacatctgc gcagcctcgg
    16441 ttacccgcag ctggagagca ttccggagag ttggaagccc tatctgcacg cagtggcctg
    16501 agttgaatta gctcaactcg acccgattgc gcccgccgct cttggcgcga tagagcgcct
    16561 gatcggcacg ctcgaacacc ttgtcgctgc cttcttcgcc ggcaaaggcg gtcaagccgg
    16621 ccgacagggt gatggtcacg cgttcgccgc gaaagtggaa tggacactgc tcgacgccat
    16681 caagcagggt ttgcagcagt tgatgaccgc cttccatggg cgttgagggg atcaacagaa
    16741 caaattcttc gccgccgaag cgggcaataa aatcggtctt gcgcaggcgc ttggccaact
    16801 cgctggcaat gatcttcaac accttgtcgc cggccagatg gccatagcta tcattgatcc
    16861 gcttgaagtg gtcgatatcc agcaccgcca gcagcaactc gccgccatag cgcttccagc
    16921 gcgccatctc caggtccaga cgttcgctcc aaccggcacg attgggcagg ccggtaagcg
    16981 ggtcgatcat cgccttttgc cgctgctctt cgaggtgatc gcggaagctc tgcgcttcct
    17041 gctccatatc ggcgaccttc tgggtcaggc tttgcagacg ccgcgctact tcttcctcat
    17101 ggccgtcgcg gtgcttctgg tgctcgctga ctgtgctgag caagccatcc aggcgcgctt
    17161 ctagggattg cttgagggtg ggcagatcga ctgcatcctg cacgctggct tgcaagccgc
    17221 tgacctgctc gcgcagttcc tgattgaagg ttcgcgcgct ttccaccgac tcgctgtagc
    17281 cctcatgggc ctcgctgagg gtggtgagaa acgatgccag acgctcattg agctgcttca
    17341 gataagtggc aaactcgcgc tgaccgctgt cggtaacggc cagcaccagc accgccagat
    17401 cgtccagcac cgccaccagt tcgtaccagt tcagaccgcc ctgcacgcgc tgacgcaacg
    17461 attcgccctg gggctgatga cgctccggca gctccagctc gtcgagcagg cccatcagac
    17521 tgtcgccgat atgcggggcg acggcgctgt aacccggctc ggggatctcc ggcagagcat
    17581 aggccgggtc gccggaatcc tgggttaatg caactagtgc cggcgccgct gctgcgctga
    17641 gcacttcggc caccgcagtc tgtgattctg gctcagcacc tgccgtggca acggctgcga
    17701 gtacaggttt aggcgcctcg ctgtgcaggt ttagcgaagt gtcggccgcc tgtaccgcgg
    17761 tttcagcttg cgcgataaca gccggggcgg acgcgccggg cggcactatc tccgccaacg
    17821 ccagatcgct gccaacagga ttgacgggcg cagcctcttt accgccaaaa agtcgctgca
    17881 aaaaacccgg acgcgcctgg ttgggctcag cctcaagcgc ggccagggcc ttttgctgca
    17941 ggctactgag ttcactgagc agcgccggca gttcacgcgg ctggctggcg cgctcttcga
    18001 tgcgtttggc aaattgcttg agcgccttgc gtacctcgcg cggcggctcc agggcgagca
    18061 gttgatcggt caggctggtg accccggaga tgttctgctc gacgcgatcc tgccggcgct
    18121 tttccgagtc cagcaccgct ttttccaggc gcggaatcag tgccgacagg ccggcgtcga
    18181 tatcgtcgcg gcgcagaata tcgcgcaact cctgcatgca ctgatcgacc gatttgtcgc
    18241 tgccctcggc ggccagcgag ctgcgcacca agccgcgtct cagcaggtcg agccgggctg
    18301 cccagctgcg ttcgagcttt tcttgctgtt ccaggctttc caggtacttg cttttccagc
    18361 gctgggtatc atcggtcatg gttgcagtcc atgttcaggg ctcaatcagc ataggcgttg
    18421 cgggcccata cagtgaagtg ggcagacgaa tttgcaccgc caccggcagg tggtcggaga
    18481 tcggctgcga cagcacctca cagcgttcca gcggcaaacc ggggctgagc aaaatgtggt
    18541 caaggcagcg ctgcggccgc cagctgggaa aggttgcctc gatctgcggg gcgagcaaac
    18601 caaggtcgcg cagcggtgaa tgcagcagca ggtcattggc atgggtgttc atatcgccca
    18661 tcaacaccat gtgctgatag tcgctcagca gctcgcggat ataggccaac tggcgggtac
    18721 gggtgcgcgc accaagggac aggtgcatca tcaccacggc aaccgcatcc ggcccctcgc
    18781 ccagacgcag caacatcgcg ccacggccag gtgggccggg cagcggatga tcttccagca
    18841 aactggggcg taaacggctg agcacgccat tgctgtgctg ggccaaacgg ccgagattgc
    18901 ggttgagttg ttgataccag taggggaagg cgccgaggtg cgcgaggtgc tcgacctgat
    18961 tgacgtagcc cgaacgcagg ctgccaccat cgacttcctg cagcgcgacc aggtcgaagt
    19021 caccgagcag atcgccaatc cgttgcagat tgccgctgcg cccggcatgc ggcagcagat
    19081 gctgccagct gcgcgtcaca tagtggtggt aacgctgggt gctgatgccc acctggatat
    19141 tgaaactgag cagccgcagg ctgccattct gcggccagtc agccgctggc gcacaacgtg
    19201 gattgacctg tggatcgcac aggtcaatca cccgtctggc cggccagcgt ctgagcatta
    19261 cgcgttatcc gcgcgcccta attactgcgc ggcgcgttct ttggcgatca ggtgatccgc
    19321 cacttgcagg gtttgctgcg ggccgcccga gctgctgata tcgaaacgat atttgccgtt
    19381 gacgatcatc accggcacgc cagtgatctg gtaagccatg gccagtttct tggccttttc
    19441 cagctggctc ttcacgccaa acgagttgta ggtcttgagg aaggcgtcct tgtcgatgcc
    19501 ttcggccgcc aggaagctgg ccatctcttc cggggtggcg agcttcttgc cctgtacgtg
    19561 gatggcgtcg aataccgctg catgcacctt gtgctcggct ttcatcgcct caagggtgat
    19621 aaacagttgg ccgtggacgt tccacatgcc gccgaacatg gctgggatac gcacgaaatt
    19681 gacatcttcc ggcaatgtgt cgatccacgg gttgatggtt tcttcaaact tgtagcaatg
    19741 gccacagccg taccagaaca gctcgaccac ttcgatcttg cccggtttgg caaccgctac
    19801 cgggttgttc agctcaacat actgcttgcc cgcttcgatt ggctcggcat gggcgctcag
    19861 gccaaacagg ctggcggtga cgagagtggc actgagaatc aggttacgca tgcgttactc
    19921 cttggctata aacggtgcgt gatgacgaac cgaagttcga cccgcgaggc aatctggggt
    19981 tccgtgcatt gtagcgatgc ccacaacgaa aaagggtggc ctgagccacc ctatttcatt
    20041 actgcatgtc gcagtgttag cgaatcaatg cagaccctgg ataaagctgg ataccgcttc
    20101 gatatccttg ttgctcagct tggctgcgat ggaacgcatg atcatgctgt cgccgtcatt
    20161 ggtgcggttg ccttcgcgga agtcagtcag ctgcttggcg atgtaggttg cgtgctggcc
    20221 acccagttgc gggtaaccgg cggcatccaa gccagcgccg ttcggcgaat ggcagccggt
    20281 gcaggcaggc atgccttcag ccagtttgcc gccgcggaac agctcgccac cgcgctcgac
    20341 cagtttcggg tcggctgcgc caacgctcat ctgctggctg gcgaagtagg cggagatgtc
    20401 ttccagatcc tggtcgctga ggttgtccag caaaccggtc atttccacca cagggcgcgc
    20461 gccggacttg atgtcattca gctgtttgag caagtaacgc tcgccttgac cagccagttt
    20521 cgggaagttc ggcgcagcgc tattaccatc ggcagcatgg cacgcaccac acacaacaac
    20581 cttaccctga ccggcttcag cattgcctgc ggcatgtgcc aggccggtga tgcccagggt
    20641 caacagcaga ctcacgagta ctttgttcat cagctaatcc aatttacggc taagagaaag
    20701 agtttataag ccgggcttac tcggtcatca atttgatgac ggcttggtaa tcctcggccg
    20761 agcagtccat gcacaggccg cgaggcggca tggcatttag gccattggtg acgctcagta
    20821 ccagcgcatc catgcccttg gccagacgcg gctcccaggc agctttatca ccacgtttgg
    20881 gcgcggtttg gatggcgccg ctatggcaca caccgcacgc acgggcgtag acggcttctg
    20941 ggtcctgtgc agcttgagca ctcaacgaca gcatcaacgc actggctgca atcagcaatt
    21001 ttttcatcag atcaacctca tcagggaggg gaacgtactg ctttctaggg gcagatgatc
    21061 taaatcgttc ccgtgaacac ttatccaaca gcgggagaaa gcgcacacaa aatctgcgcc
    21121 attatatact ggcgtcattg aaacggaaac gacgcagctt gctcgctacg cggcaacagg
    21181 caggctgcgc gcaaatgtcg caagggcatc gcgcctgtaa cgccacccta cgctcgccct
    21241 cacggatacc cagatgcaag ccaagaaccc cattatcggc ctgtgccaac aggctacttt
    21301 ccttatcagc gcggccaaag ttgaccagtg ccctgaagat ttcggccatg aagtggcctt
    21361 tgccgggcgc tccaacgccg gtaaatccag cgcgctgaac accctaaccc acgccagcct
    21421 ggcacgcacc tcgaagacac cagggcgtac ccagctgctc aacttcttca agctggacga
    21481 agagcgccgc ctggtcgact tgccaggtta tggctacgcc aaggtgccaa tcccactcaa
    21541 gctgcactgg cagaagcacc ttgaagccta cttgggcagc cgcgaatgct tacgcggcct
    21601 gatgctgatg atggatatcc gcaaaccgct gaccgagttc gatatcctca tgctggattg
    21661 gtcagttgcc agcgaaatgc ccatgcatat cctgctgacc aaggccgata agctagcttt
    21721 tggtgcagcg aagaacacct tgctcaaggt gcgccaggac attcaccagc gttggggcga
    21781 ccgcgtgagt atccagctgt tctctgcgcc caagcgcatt ggcatcgaag acgctcagga
    21841 agtgctggct cgatggatgc aactgggtga gtttgccctg ccggaagatg aagaagtcga
    21901 cgcctgataa agcgctcgct ggctggtagc cataaaaaaa ccccgaaact tcttatgggg
    21961 gagggaagtt tcggggttca acatctgaac cgctagggcg gggtccagaa tctgccaaca
    22021 cttaacacaa caaaggagca tcgaatggtt gtctaggcca ttcataagct ctgagtaggc
    22081 aggcagacgc aaagttcaaa agcccgctgt aattaattag ttataagcag gcccgtattg
    22141 atacccatac gcctttagcg cccgtcagcc gggcgctgca gccaagttgc gggggttcga
    22201 attaatgggc ttcatcccag ttatcgccta cgcccacttc caccagtagc ggcacatcca
    22261 gtgcggcggc attgcccatc aacggcctga tctgcgcgct gacctgttcg accagatcct
    22321 cacgtacctc cagcaccagt tcgtcgtgca cctgcaggat gactttggca tccaggccgg
    22381 attcgctcag ccagttgtcc acggtgacca tggcgcgctt gatgatatcg gccgccgtgc
    22441 cttgcatcgg cgcgttgatc gcagtgcgct cggcaccttt gcgcagcgcc tggttcttcg
    22501 catggatatc cggcaggtac aggcgacggc cgaacagcgt ctcgacaaag ccctgctcgg
    22561 acgcctgctc acgggtgcgc tccatatagg ccagcacgcc ggggtagcgg gcgaagtaga
    22621 cgtcgatata ggcctgcgcc tgcttgcggt cgcagccgat ctgcttggcc agaccgaagg
    22681 cgctcatgcc atagatcagg ccaaagttga ttgccttggc gctgcggcgt tgatcgccag
    22741 taacttgatc cagcggcaca ccgaatacct cggcagccgt agccttgtgc acgtcgcggc
    22801 catggcggaa agcatccagc agactttcgt ctttggccag gtgggccatg atgcgcagct
    22861 cgatttgcga atagtccgcc gccagcagct tgtagccctt gggcgcgacg aaggcctggc
    22921 gaatgcgccg gccctcggca gtgcgaatcg ggatgttctg caggttcggg tcggtggacg
    22981 acaagcgccc ggtcgcggcc accgcctggt gatagctggt gtggatgcgc ccggtacgcg
    23041 ggttgatctg ctccggcaaa cggtcggtgt aggtgctttt cagcttgctc agggtgcggt
    23101 actgcatcag caccttggga agctcgaaat cctgttcggc caattcggcg agcaccgctt
    23161 cggccgtcga tgcctggccc ttggcggtct tgctgatcac cggcaaacca agtttctcgt
    23221 agaggatcac cccgagctgc ttgggcgagg ccaggttgaa ctcctcacca gcaatctcga
    23281 aagcctgacg ctccagcgcc accagcttct cgcccagctc gttgctttgc aggccgagca
    23341 gcttggcatc caccagcgcg ccctggcgct cgatacgcgc cagcaccggc accagtggca
    23401 tttcgatttc ctgcaacacc ttggccaggc tcgactcggc cgagagcttg gcccacagat
    23461 gctggtgcag acgcagggtc acgtcggcat cttcggcggc atagggcgcg gcctgggcca
    23521 gatcgatctg gtcgaaggtc agctgtttgg cgcctttgcc ggcgatgtcc tcgaagcgaa
    23581 tattgctgtg atccaggtac ttgagcgcca ggctgtccat atcgtgacgg gtggcggtgg
    23641 aatccagcac gtaggattcg agcatggtgt caaaggccac gccctgcacg cggatcggcg
    23701 tcgaggcatt ggcgaggata ttgatgtcgt acttggcgtg ctgaccgacc ttggcttttt
    23761 tcgggtcttc cagaatcggt ttgagcgcct gcagcaccac atcgcggtcc aactgcgtcg
    23821 gcacgcccat ataggaatgc gcgaccggga tatacgccgc ctcacccgcc ttaaccgcaa
    23881 aggacaggcc aaccaactgg gccttttgcg cgtccaggcc ggtggtctcg ctgtcaaagg
    23941 caatcagctc ggcgttgcgc aatttctcca gccattcgtc gaactgcgcc tgcgtcagca
    24001 cgcactggta atcacccagg gctgtcgcgg ctggcaattc gctaactacc gcgtcggcag
    24061 tcggtgcatc agcggccgct ggagctggcg cgactacagc ggcggcactg gcgctaggcg
    24121 ccagagcaaa caagtcatcg gcaggcgctg cgaccggcgt tttgaccact ggagcagggt
    24181 tgctgcgcag caactcatca cgccaggttt taaactccag ctcggtgtac aactcgacca
    24241 gggctggcac atccatctcg ctcgggtgca gcgagtcgat ggcgatattc agcggcacat
    24301 ccagtttgat ggtcgccagg gcgtaggaca gatacgccat gtccttatgc tcagtgagtt
    24361 tggcaggcag ggtcttggcg ccgcgtatcg gcagttccgg cactttatcg aggttggcgt
    24421 agagcacatc gaggccaccg ccgaccccta ccagcaggcc cagggcggtc ttttcaccga
    24481 cacccggcac gccgggaatg ttgtcgacct tgtcgcccat cagcgccaag taatcgatga
    24541 tcagctcagg accgacaccg aatttcgctt tcacgccatc gatgtcgtag acgctaccgg
    24601 tcatggtgtt gaccagcgta acgtgcgggc agaccagctg ggccatgtcc ttgtcaccgg
    24661 tggagatcac cacgtcgcgc ccctcggctg cgcactggcg ggccagggtg ccgatcacat
    24721 catcggcctc aacgccttcg acgcacagca gcgggtagcc catggccctg acgctggcat
    24781 gcagcggctc gacctgtacg cgcagctcat ccggcatcga cgggcgattg gccttgtact
    24841 cggcgaacat ttcatcgcga aaggtcccgc ccttggcgtc gaacaccacg gcaaatgggc
    24901 ttttcgggta ctgcttgcgc agactcttga gcatgttcag cacgcccttc accgcgccgg
    24961 tgggcaagcc cttggaggtg gtcagcggcg gcagggcgtg aaaggcacgg taaaggtaag
    25021 acgaaccgtc aaccagaacg agaggggctt ggctcatgag caggatcaac cttttcggcg
    25081 ggggcagcgg taacaggccg tttaaaaacg taagcgaggc cggcaagacc aggcaaaaac
    25141 aggcgaggaa gcggagttta cgtgctgtaa atgagcattc cgagcctgtt tttaacgcag
    25201 gattgccaac gcaggtagtt tttcaacagc ctgttagaat ggccgcacca ttgaaatcaa
    25261 aaggacaagg ttatcatgcg cgcactcaat cgcctgttgc tcgccagcct gctggcattc
    25321 accaccctgg tcgcccacgc cgaagacccg gtttcggccg acccggatgt caccattcgc
    25381 caggacggcg accgcaccat ccatgaatac cgggtcaacg gttttctcta cgcggtcaag
    25441 gtcatcccca aaggcggcaa accgtacttc ctggtacgcg ccgatggcag cgatggcaac
    25501 ttcgtccgtt cggacagccc ggatatgttg attccggcct gggaaatttt cagctggtaa
    25561 gaaggcagtt ctgcaatgtc ggtgtttacc ccgcttgagc gccacgagct cgaggcgttt
    25621 ctcgccccct atgacctggg tcgcttgcgc gacttccagg gcattgctgc gggcagcgag
    25681 aacagcaatt tcttcgttag cctggagcag ggcgaattcg ttctgaccct gatcgagcgc
    25741 ggcccgagtg ccgacttgcc gttctttatc gaactgctcg acgtgctgca cgacgcaggc
    25801 ctgccagtgc cttatgccct gcgcacgacc aatggcgagg cgttgcgcag cctggcggaa
    25861 aagccggcgc tgttgcagcc gcgcctggcc ggcaagcatg tgcacgacgc caatccgcat
    25921 cactgccagg aagtcggcgc actgctggcg cgcattcacc tggccacgcg caatcagccg
    25981 ctgccgcgca aaagtgatcg agggctggac tggatgctgg ccgaagggcc gagcctggcc
    26041 ttgcagctgc ccgatgaaca gctgccactg ctgcgtgatg cgctggcgga aatccacgca
    26101 ctcaagccgc gcatccttgc cttgccacag gccaatctgc atgccgacct gttccgcgac
    26161 aacgtgttgt tcgacggtaa ccacctggcc ggggtgatcg acttctacaa cgcctgctcg
    26221 gggccgatgc tctacgacct ggcgatcacc ctgaatgact ggtgctcttg cgaagacggc
    26281 agcctcgaca gcaaacgcgc gcaagcgctg ctgggagcct atgccggctt gcgggcattt
    26341 actgcggcgg aagcggagct gtggccggcc atgttgcgga ttgcctgtgt gcgcttctgg
    26401 ctatcacgcc tgatcgccgc cgagtccttc gccggccagg aagtgctgat ccacgacccg
    26461 gcagaattcc agcgccgcct ggcccagcgt cagcaggttg agctagcgct gccgttcgcg
    26521 ttctaggaac ggaggctgac tacagcgcct ccagacaacc cgccaatccg cccgccagat
    26581 tttccagcaa ggcttcatag ccctttgcat ccactggcaa ggcgccgccc atagcgtcca
    26641 actcagccag ggtgaccggc aggccggcgg tcagggtttg cgccaggcgc gggcggaacg
    26701 gcggttcgct gaatacacaa ctcggtccgg cttgctgcaa gcgctcgcgc atggcggcaa
    26761 cgtgttgggc acccggctgc acttcaccga gcacgctgaa cacccccgcg tgcttaagcc
    26821 cataggctgc ctcgaagtag tcgtaggctt cgtggaaaac gaagtagggc ttgcccgcca
    26881 ggctgctcaa gcgggttttc agacgctgat ccaacgcatc cagacgctcg ccgaatgcct
    26941 tggcattggc ctggtaacgc gcggcattgg ccgggtcggc ttgcgccagg tcggtggcga
    27001 tcttgccggc aatcactcga gcattagcgg gcagcagcca gagatgggca tccaggctac
    27061 cagggcgatg cgcatggtca tgc
//
LOCUS       DASRUM010000305        31984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2497011, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000305 DASRUM010000000
VERSION     DASRUM010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 31984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31984
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2497011"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1754)
                     /gene="rbbA"
                     /locus_tag="VFF22_20555"
     CDS             complement(<1..1754)
                     /gene="rbbA"
                     /locus_tag="VFF22_20555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017246010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HZX19245.1"
                     /translation="MSAPAVPVAQLQDVSLQYGKTCALKAVSLEIPAQCMVGLIGPDG
                     VGKSSLLALIAGARKIQSGQIEVLGGDMASRAHRRQVCPHIAYMPQGLGKNLYPTLSV
                     IENLDFFARLFGQAEAERKTRIAELLRSTGLSEFGERPAGKLSGGMKQKLGLCCALIH
                     DPDLLILDEPTTGVDPLSRNQFWELIERIRRQRPQMSVLVATAYMEEAERFDHLLAID
                     DGAVLATGSPAELLQRTQCSSLEEAFIALMPEAKRQGHKQLVIPPRPAGEQEIAIEAR
                     DLSMRFGDFVAVDHVDFRIERGEIFGFLGSNGCGKTTTMKMLTGLLPASEGQALLFGQ
                     VVNASDMATRKRVGYMSQSFSLYAELSVRQNLVLHAQLFHVPATEIAGRVTAMAERFS
                     LGDVMEQLPERLPLGIRQRLSLAVAVIHKPEILILDEPTSGVDPIARDVFWQMMLDLS
                     RNDGVTIFISTHFMNEALRCDRISLMHAGKVLDSDTPKALMAKRGLPTLEATFIAYLQ
                     DAVGEKAQQPAPEAAAPAAQTNTLKRKRFSLLRLFSYTRREALELRRDPIRGSMALLG
                     SLLLLFIIGYGISLDVENL"
     gene            complement(1751..2821)
                     /locus_tag="VFF22_20560"
     CDS             complement(1751..2821)
                     /locus_tag="VFF22_20560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZX19246.1"
                     /translation="MSVSRKWIFPAASVAVVAIIAGLLWYTLRPEGLGDGFASGNGRI
                     EATDVDAASKLPGRIASIEVDEGEFVEAGQVIARMDTQVLQAQVLQAQAQMRQAQNSK
                     LTASAQVRLRESEKVTAQAIVRQRQAEVSAAQKRHARSAILVKRNAMAQQQLDDDLAR
                     LQSTQAALAAAQSQVISAEAGIAAAQSQVIEAQSAVEATQASVERLQIDIEDSQLKAP
                     RAGRVQYRIAEPGEVIGAGGKVLNLVDLSDVYMTFFLPEQQAGRVALGAEVRLVIDAV
                     PQYVIPAEVSYVASVAQFTPKTVETASEREKLMFRVKARIDPELLQRHLTQVKTGVPG
                     VAYLRLDPQAEWPAELAIKVPQ"
     gene            complement(2886..3191)
                     /locus_tag="VFF22_20565"
     CDS             complement(2886..3191)
                     /locus_tag="VFF22_20565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003221201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HZX19247.1"
                     /translation="MMSSQILQLDIHEFCLCAELPQSVLLEIVEHGIVEPCGATPEQW
                     RFSSHALSVAKRALRLQVELQLEWSGVALALQLLDEVEHLRAENSHLRRRLSRFEQQ"
     gene            complement(3188..4135)
                     /gene="cbpA"
                     /locus_tag="VFF22_20570"
     CDS             complement(3188..4135)
                     /gene="cbpA"
                     /locus_tag="VFF22_20570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009394320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HZX19248.1"
                     /translation="MDFKDYYKVLGLEPGADDKAVKTAYRKLARKYHPDVSKEADAEN
                     KFKEVAEAYEVLKSAEKRAEYDELRKYAEQGRPFEPPPGWQPSGHAGFTGGNSAGSEY
                     SDFFESLFGGAGRFQDAGRNRVRRGQDVEMALPLLLEETLADETKTIAFQLPQYSADG
                     QPLPAISKTLNVKIPPGVSNGERIRLKGQGAPGVGGGAHGDLYLIIHFVPHPLFDVEG
                     HNLVLTVPLAPWEAALGSQVTLPTLSGKINLRIPANTQAGQRLRIKGKGLRNKAGEPG
                     DLYALFKIVIPAQADEPVRALWTQLAEQAAFNPRAQWSQ"
     gene            4540..5049
                     /locus_tag="VFF22_20575"
     CDS             4540..5049
                     /locus_tag="VFF22_20575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HZX19249.1"
                     /translation="MGFEQLAELRDRLRAENVQVKTDSPKQRTRKSSPQKKLHEQDPA
                     VEAIWLLQKHFPLAFPVSPAPKVPLKEGILKDAEQHLELLGITSEQLKQGIATWCRGS
                     RYWASMTENAPRLDLTGQAVGIVTVAQALHAKHQALRQRGQARRNQTKPKALLQEKAV
                     DTPVEQIVD"
     gene            complement(5232..6470)
                     /locus_tag="VFF22_20580"
     CDS             complement(5232..6470)
                     /locus_tag="VFF22_20580"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX19250.1"
                     /translation="MRLNAAIVVLLTSLVAATYGFGIYLFAQLVPDMQASLGFDFSYV
                     GSITASAQLGFLICALLAVWLTPKIGGGWMIVASGAVCALALMLIPLSNNILVIGALL
                     TLLAGTAATVFVPMVDVVARVTAYRYRGLAMGLVSSGTSYGVAINSLLVPLYAAQGEW
                     RSVWWWVGLLTVAMALLVLVVFKKGGLLSPTVTPAISPTDVELTVPNRRLTLIKPWVL
                     LIWSMNFLIGFATFPFQTYLSSYLRTELGFDVQFSAQVWAVIGFVGMFAGLAVGWLSD
                     RTGLRAAMLLVYGCVVTAALIFVIHPSGYWPLIAAGLFSTAFYPIFGLIPAYVSKLAS
                     SSAMAVSIFGVANVMQGSGGMLGNYGAGLLANHSGSFAGVYAAISGVGVLLIVLTLKL
                     PKEAPVATSVGLAANYPTAS"
     gene            7123..9528
                     /gene="pqqF"
                     /locus_tag="VFF22_20585"
     CDS             7123..9528
                     /gene="pqqF"
                     /locus_tag="VFF22_20585"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqF"
                     /protein_id="HZX19251.1"
                     /translation="MSVRLFSQPWLSQVALCLRVYAGSHDEPLAYPGLAHFLEHLLFL
                     GSVGFTGEQRLMPFVQACGGQVNATTQARYTEYFCEVPAECLEGALERLLDMLARPLL
                     GEVEQVREREVVHAEFIARSQDVDTLLGAAMGQALADGHRCGVFQAGNRDTLPVTDAA
                     FQQALRAFHQAFYHAGNCQLSIVAPSSLEQLNALVQRHRQLLPEARVFVRSRVAPLLP
                     LRAERLRLNLPGVAASLHVGFALELPEARADSALDWLLNWLLDEAPAGLKAGLREQGL
                     GELLRAQVLYCHAGQALLLLSVHGVAAPDEAVALLLDWLAFVSARADLVAWHDAYQQI
                     CQQRLFGMTPLTLARNWQAPEHKEGGAMRMLLQQLQQPQRRCVLLADNRELPHWPGAG
                     FPLQMRAEPASSVPVLRGRWQLPARNPLLAEAEVPGVRLAVPAAFRWLAAPVHYAVPA
                     AAVAVWQARCCFVEPLGANILLNLAHTGLRAVRQHLQHVGISLSLEAETASLSVRLQG
                     SARLIPGAVVLLMPALLQPHPAQWSALAVAEPPSMPIRQLLAASAELLQGQAIDLADE
                     AVATLQRRYRQVRIEALGVGLDRPGQALIESLFRTSAPWPSVAIAPLQPGLHWRTRAA
                     HGDSALLLFCPQVDDSPATEACWRLLAHLLQGAFYQRLRSELQLGYAVFCSYRQIQGR
                     RGVLFGVQSPGCKAAEILQHIQVFLQARCTWLAGVDQAALIAALTALRGQWQAQARSC
                     DGLAEQAWLAHLAGLSAAHGEAVQQALQQLSLETVQQALQALLEASSGWYVLSNEAAP
                     GHA"
     gene            9874..11058
                     /locus_tag="VFF22_20590"
     CDS             9874..11058
                     /locus_tag="VFF22_20590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013693515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZX19252.1"
                     /translation="MNNNKIASTRFLPLTLAAAVAMVTAQHAAAEIVLYDKDDTTFST
                     DGYINAFYVNSDVDREGEQFDRRQSRVKMGFLPNWIGFNFGKQIDDLKLGGRSSFWVT
                     INDSESNGTDTAIDVRQFYGTVASPEWGEVLVGKDFGLFSRSNIFLDELLAGYGNVSD
                     TLGLVDGTGVSFGNIGTGYPYPFPTSQITYRNNNLAEGLRVAVGIMDPVDTNDDSAVG
                     KSYQEEPRFESEVSYQFDVGGATIYSWLNGAHQTSDNTDKAVDSVTSQGLGYGVQAKM
                     AGFSVTASGFQAEGINPFFTNNAGEATLREIDSEGYLLQGSYTFGKNRVALSYGKTED
                     DGNGLGTAADYETRGVAYFRTINDNLKLVAEYNQYEIDAAEGSGLGEDTNTFAVGAVL
                     SW"
     gene            11222..11614
                     /locus_tag="VFF22_20595"
     CDS             11222..11614
                     /locus_tag="VFF22_20595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020298071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX19253.1"
                     /translation="MLGLWSAHAQQYLLWLTLATTLVFALPIFCIPLVWARLMGWTLP
                     SHTDLAVYFGRCLGAFILILEAMMLRAALSGEAIRAVFEMLLAVAVMMIIVHVYGALR
                     RIQPLSETLEIGFYTGLLVLTLLFWPAT"
     gene            11709..12863
                     /gene="nosP"
                     /locus_tag="VFF22_20600"
     CDS             11709..12863
                     /gene="nosP"
                     /locus_tag="VFF22_20600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035991 - nitric oxide sensor activity
                     [Evidence IEA];
                     GO_process: GO:0046209 - nitric oxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing protein NosP"
                     /protein_id="HZX19254.1"
                     /translation="MPHTLTEGVVTATSNAADVELVAQELARQLIHPHLGFVLFFCSA
                     EYDLQGLGEALDQYFGGINLVGCTSAGEITAQGYDRGCVSAIGFDHRSFSIASALIDE
                     MERFSLIDAQQLVERLVSDCRNNELAAIKGHSFALTLLDGLSSREEVVLGALSAAFGS
                     IPHFGGSAGDDNHLTHTHVYYSGEFHTGAAVVVLFNTWLDFEVFSTHHILPRSEKLVV
                     TRADSTSRRVYELNAAPAALEYAELIGVPLAALDHRLFAAHPLAVRIGGQYYVRSIQR
                     VNDDLSLTFYCAVENGIVLTAMQPGPLLPDLQGVFEGLRERLGPLLLTIGCDCFLRRL
                     EIERRDGVEPVSAFLRSQNVIGFNTYGEQFNGMHINQTFTGVAIGRPGRG"
     gene            12802..15438
                     /gene="nahK"
                     /locus_tag="VFF22_20605"
     CDS             12802..15438
                     /gene="nahK"
                     /locus_tag="VFF22_20605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator NahK/ErcS'"
                     /protein_id="HZX19255.1"
                     /translation="MVCTSTRPSPESPLAVPVAADVAELQARCLELERQNGKLRQINS
                     ALIERVESVHSRGNDGYAAFQHSVVLAEQVRERTDALNQAMAELKSSNQLLSDARLRA
                     ETAHQHLIDAIESISDAFVLFDREQRIVLFNSRFKSFWAGSRARISAGTRLAEIKRLS
                     KSTGLVVEEQRSNRDDNMLYRLQDGRWVQVSERPTREGGLVILYTDITEVKLNETMRR
                     EQALAQKSRLLQRAMDNLSQGMAMVNADGQLELWNHRFLDLCGLAPISAHRPFAEVMA
                     ESELELLTPASRDANGRLIEELELCLFDGRMLEVRTHPLPTGGFVNTFTDITERHRHA
                     KALSESERWIRLITDHVPALIAYLTADLVYQFTNKVYEEWYCWPRSAMLGQSLRVVHS
                     EEHCRRLEPFIERALSGESVTFEFAETNHHGQERYMLRSYVPNRQASGEVVGIFVLIR
                     DITERRRTAEALHQAYQHLEQRVRERTAELTELNSQLRREIDERTQMEARLREAKGEA
                     EQANLSKTKFLAAVSHDLLQPLNAARLFTSALLEKPDLADSATLVRNVSNSLEDVESL
                     LGTLVDISKLDAGVIKPDIAPFAVGELLENLAAEYHQIAGSEGLRLDFIPSSALVRSD
                     IQLLARILRNLLSNAIRYTSSGRILLGCRRRRQSLSIEVWDTGIGIADDKLGEIFQEF
                     KRGDVSHRKQDRGLGLGLAIVEKIARMLGHRIRVASRQGKGSMFAIEVPLTSLAPRTR
                     AVMDAPNPLVERLSGSRIWVLDNDAAICAGMRTLLETWGCQVVTALSEEDLARQVDNF
                     HADADLLIADYHLDDDHNGVDAVAQINARRSSPLPALMITANYSNELKQQMRELGHTL
                     MHKPVRPMKLKTAMSHLLERRE"
     gene            complement(15506..16033)
                     /locus_tag="VFF22_20610"
     CDS             complement(15506..16033)
                     /locus_tag="VFF22_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZX19256.1"
                     /translation="MSNYRRAHEGRLYFFTLTTEQRRPLLTQPELRIALRQAIEQVRQ
                     RYPFSIHAWVLLPDHLHCMWALPEHDTDYGRRWSLIKRQTSQQLALPGTVSLSRHLRR
                     ESGLWQRRFWEHQIRDTQDYQRHLDYVHWNPVKHGLVNRVADWPWSSFHRLVRQGLYP
                     ADWGGGRSDEGDLGE"
     gene            complement(16171..16836)
                     /locus_tag="VFF22_20615"
     CDS             complement(16171..16836)
                     /locus_tag="VFF22_20615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZX19257.1"
                     /translation="MYKILIADDHPLFREAIHNVISDGFPGSNIMETADLDSALALTQ
                     EHDDLDLILLDLNMPGMHGLGGLINLRNEAPTIPVVIVSAEQDKQIVLQAITYGAVGF
                     ITKSSPRAQMTDAIQQILNGNVYLPPDIIRTQKSGSQRSHTDHHSFPSELLQALTRKQ
                     LLVLERMTKGESNKQIAYSLDIAETTVKAHVSAILRKLNVHNRVQAILSAGDIDFTAY
                     LRR"
     gene            complement(16892..17437)
                     /locus_tag="VFF22_20620"
     CDS             complement(16892..17437)
                     /locus_tag="VFF22_20620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012052075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated CXXCW motif
                     protein"
                     /protein_id="HZX19258.1"
                     /translation="MKHWPKALILIASLLPTSPVLADTPLFSADGYRLSHYRSPTPLT
                     SEHARILDTAALQQLLREQPGTRLIDVYRRQWLHGQFIEDETHANLPNSLWLANTGDG
                     ELNAQWQTYFHQHLTQASQGNRAWPLVFYCRSDCWLGWNAVKRAHHLGYTNLYWYRDG
                     IDAWQQAGLPLIPAQPLPLLR"
     gene            complement(17577..18371)
                     /locus_tag="VFF22_20625"
     CDS             complement(17577..18371)
                     /locus_tag="VFF22_20625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZX19259.1"
                     /translation="MNTYWYCLQGIVQREWLRFVLQRSRFLSALVRPLLWLLVFAAGF
                     RAALGIAIIEPYDTYITYETYIVPGLACMILLFNGMQGSLSMVYDREMGSMRVLLTSP
                     LPRAFLLAGKLLAIALISLLQVYAFLAIAWVYGVQPPLWGLLSALPALLLVALLLSAL
                     GLLLSNGIRQLENFAGVMNFVIFPLFFLSSALYPLWKMREASEWLYWLCALNPFTHAV
                     ELVRFALYQRFSPLALAVCLGLTLLFAVLAVLTFNPQHAALRKGNG"
     gene            complement(18445..19158)
                     /locus_tag="VFF22_20630"
     CDS             complement(18445..19158)
                     /locus_tag="VFF22_20630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020300007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZX19260.1"
                     /translation="MNALQLEGVSFAYGSRPALQEVAFSLTTGRFAALLGPNGAGKST
                     LIALLTRLYDLQHGDIHIFGHSLRTTPRPALRQLGVVFQQSTLDLDLSVQQNLAYHAA
                     LHGVSKRTAQARIDEELERHTLTERRHEKIRALNGGHRRRVEIARSLLHRPRLLLLDE
                     ASAGLDPASRLALNQHVRQLSRDEGLSVLWTTHLLDEIEPGDDLLILNHSRLVAQGQA
                     EDFGHDLAASFARLTTPCA"
     gene            complement(19155..20120)
                     /locus_tag="VFF22_20635"
     CDS             complement(19155..20120)
                     /locus_tag="VFF22_20635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YVTN family beta-propeller repeat protein"
                     /protein_id="HZX19261.1"
                     /translation="MRLTHLACAIALGLASHTALATTAYVSNEKDDSISVIDLGSLEV
                     TATLDVGMRPRGLLLSSDNKLLYICASDSDRVQVMDLATRTIIKELPSGADPEQFALH
                     PNDRWLYISNEDDALVTVVDTQSDEVLGQIDVGVEPEGMAVSPDGKWAVNTSETTNML
                     HWIDTTTQQLVDNTLVDQRPRHVEFDKNGKRLWASAEIGGTVSVIDVASRQIEKVLTF
                     QIKGVHPDKVQPVGVKLSDDGKYAFVALGPANHVAVVDAKSYEVLDYLLVGRRVWHLA
                     FTPDQKRLLTTNGVSGDVSVIDVDTLKVSKSIKVGRYPWGVVVTP"
     gene            complement(20194..21381)
                     /locus_tag="VFF22_20640"
     CDS             complement(20194..21381)
                     /locus_tag="VFF22_20640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZX19262.1"
                     /translation="MLCAAVLCSLALPCLATPAADVLQVRIAYLGYTPASGPLLSNVI
                     PEPSDAGRRGAELAISDSNSTGHFLKHSYQLDVAEHTDGASLLTAARELHAQGVRLFV
                     VNAPASSLRQLSQALPDSLLLNAGSADDSLRQRQCLNNVLHTLPSRAMLADALAQFLA
                     VRKWTRWLLISGSTEDDQAYASALKRAAKRFGHRIVAEKTWSFDNDQRRSAQAEMPLF
                     TQTTEYDVVLVADERGDFGEYLPYNTWYPRPVAGTQGLTPTAWHKTVETFGAAQLQKR
                     FEALAGRWMNDRDFAAWMAVRSIAAAVTKLRATEPQAIRNLALTAELPLDGFKGRKLS
                     FRPWNGELRQPIPLVQPRALVSTSPQDGFLHPHNEMDSLGYDQAEVSCDLTRGGQGTP
                     YSP"
     gene            21563..22000
                     /locus_tag="VFF22_20645"
     CDS             21563..22000
                     /locus_tag="VFF22_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HZX19263.1"
                     /translation="MSTLKTLALALILLSSLIGFDSIYAAGDLTRRTEKLPDLLLGSE
                     ASDYSLSQSEYQLETGKAYQLKIIASGQKEYAFQAPEFATSIYLRKVEAGGVEIKAIT
                     LIELEFEDAGEAEIFFVPIKPGTFRFYAKGLEGKGMLGHFVVK"
     gene            22002..23255
                     /locus_tag="VFF22_20650"
     CDS             22002..23255
                     /locus_tag="VFF22_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020193878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZX19264.1"
                     /translation="MSVLWRIYLLVTLFFVLVSVVGLALLLRQAREDVQREVQAAEAV
                     VEYLHEAARRDPASLQSGLTGNLRHVRVQWLPAPATTPDASFDQWLGQRLFPLEHRPR
                     RVQLADGRLLQIAVDPTDEIDEVWDSLLQLLLLCLLALLISLVAIRWAVHRGLRVLDE
                     LLGGLHQVTQGRLDARLPAHSLPEARQLAGHFNAMATTLQQVQADNTELTQALMALQE
                     RERARLGQTLHDDLGQYLSGIRAQACLLQAVADQPEQVRLTAQHLDNHSQRLQEGFRA
                     LIRDLYPVVLERLELDEALQLLADNWQAAQRVQCRVMLSRHLPSLPLPTKAHLYRLVQ
                     EALTNVARHAQASEVRVRLHYQGQRLRLFIRDNGRGAQLPQRPGIGLRSIHERARSLG
                     AVLRVHSRPHAGWALCLTLALPEPE"
     gene            23255..23905
                     /locus_tag="VFF22_20655"
     CDS             23255..23905
                     /locus_tag="VFF22_20655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZX19265.1"
                     /translation="MKILLVDDHAVVRQGYASLLRALLPDVQVREACDGEQALQMVQE
                     EIPNLVIMDIGLPGISGLEATRRLRQRLPQLRVLFFSMHDELPLVRQALDAGALGYLT
                     KTSSPEVLVEAVKRTLAGHAYIEQSLATHLACNPLGEGSSDPRLRGMTQREFEIFVML
                     AKGMPSRQIAERLCISGKTVSNYLTLLKSKLQVSSQAELVHLAIDTGVIRVGLESA"
     gene            24350..25414
                     /locus_tag="VFF22_20660"
     CDS             24350..25414
                     /locus_tag="VFF22_20660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX19266.1"
                     /translation="MSNRANFLEPTKQALAKRAGNSCSFPGCDAITEGPSSESDSSVS
                     KTGMACHIYAAANGPSARRVNLQLSDAQLSEISNGIWMCYSHGKLIDTDEATYTVEQL
                     KTWKVISELRARLWQQLGKRIELPPESFKCIPLPETKIGFESLGQENILIGEAISRAC
                     VSQVWGKPESQAIRDTLIELTRNAFGHGKASCLSIQIKPKSIVLTDDGTKYDPIKIFT
                     ENHQGGGFHSIDELVNNHAGSVYFSFHNDGNKNNYIFSIVSSPNDIKELTPCHIEIPR
                     EYYWEEGVKFEVAEFCDTVYVIFPDFFALSDALKLPVLIEEHLPKGKDYVFVGVDLSQ
                     RAIEIIESRVENIKILNYRD"
     gene            complement(25581..26228)
                     /locus_tag="VFF22_20665"
     CDS             complement(25581..26228)
                     /locus_tag="VFF22_20665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HZX19267.1"
                     /translation="MNYLPLLLPTVLLILGTELVNADEVLTINGCRIEADSQCPNADL
                     RNANLSNQDMRRMNLAGADLRGADLRHSRLDLANLEKARLQGADLTRASLQQSNLRLA
                     NFSNSTLVAINGWALFAQAAQFEGADMRAANLEFARLSAAKMHAVNLQAANLEMTWLS
                     KADLKGADLKDANLQEVKLSGSNLANADLSGTRTHYGNFQGANMEGCVECPKGWD"
     gene            complement(26367..28238)
                     /gene="exaA"
                     /locus_tag="VFF22_20670"
     CDS             complement(26367..28238)
                     /gene="exaA"
                     /locus_tag="VFF22_20670"
                     /EC_number="1.1.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein ethanol dehydrogenase"
                     /protein_id="HZX19268.1"
                     /translation="MTTRSLPSMPRPVALAVHSLLLAGGLALSGSALAKNVTWDDIAN
                     DHTTTSNVLQYGMGTNAQRWSPLAQVNDQNVFKLTPAWSFSFGDEKQRGQESQAIVHD
                     GVIYVTGSYSRVFALDAKTGKRLWTYNHRLPDNIRPCCDVVNRGAAIYGDKIYFGTLD
                     ARVVALNKDTGKVVWNKKFGDHAVGYTMTGAPTLVKDQKSGKTLLIHGSSGDEFGVVG
                     KLFARDPDTGEEVWMRPFVEGHMGRLNGKDSTPTGDVKAPSWPDDPNHPTGKKAAWSQ
                     GGGAPWQSASFDAETNTIIIGAGNPAPWNGWARTADGGDPKDYDSLYTSGQVGVDPTT
                     GEVKWFYQHTPNDAWDFSGNNELVLFDYKDKAGKTVKATAHADRNGFFYVVDRANGKL
                     QNAFPFVDKITWASHIDLKTGRPVENEGQRPAKPEPGEAKGKSVEVSPPFLGGKNWNP
                     MAYSQDTGLFYVPANHWKEDYWAEEATYKAGSAYLGMGFRIKRLYDDHVGILRAMNPT
                     TGKVVWEHKEKLPLWAGVLATKGNLVFTGTGDGYFKAFDAKNGKELWKFQTGTGIISP
                     PITWEQDGEQYIGVTTGYGGAVPLWGGDMAELTKPVAQGGSFWVFKLPSWDNKTAQR"
     gene            28548..28967
                     /gene="pedF"
                     /locus_tag="VFF22_20675"
     CDS             28548..28967
                     /gene="pedF"
                     /locus_tag="VFF22_20675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-550 PedF"
                     /protein_id="HZX19269.1"
                     /translation="MTIKKNTGFALLLLAFSTNLWAHGDVVPQAVKTEGLDPLGEEWL
                     EENPYRGNPRAIEIGSSAYNQNCAACHGLEAKSGGIAPDLRLLEAGITGDEWFKERVI
                     NGAVRDGRVYMPKMADYLSQEALWAVRSYLESVAIEE"
     gene            29030..29869
                     /locus_tag="VFF22_20680"
     CDS             29030..29869
                     /locus_tag="VFF22_20680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZX19270.1"
                     /translation="MPLQAQVRSYDSIVESGVLKVALYQDFAPYSFQQDGQPRGVDYD
                     VAAALAEGLGLTLEVIWAPPGEKLDDDLRDYIWRGHYLRPHELADVMLRVPYDREFSY
                     KSNEFGELVNELVVMFGPYQRERWQVAFDRRRLDAVPSVAVFQRHPIGVEVESVPSFY
                     LSSVKDGMLSQQTHHYGNVPQAFAAMKTGEVDAVMALRGEVDWQLKLAADSQLALAEN
                     AYPDMGKQVWDIGMAVHESNRQLAYALEEKLEELILAGALKRIYGHYGLRYELPGLYQ
                     EVE"
     gene            29908..30657
                     /locus_tag="VFF22_20685"
     CDS             29908..30657
                     /locus_tag="VFF22_20685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014591384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HZX19271.1"
                     /translation="MDWRSFLLLALFAVPWDSWGSQRDPVTSVMWDYYHERLLGGEAF
                     VFDERVKLSAPPFAEDARQVPIQVDASAFDGQVVRMLAWAELNPIAQIFDFQPGEQVL
                     PRLAIRIRVEQATPLRVAVLTRDGLWHIGSTQIEAAGGGCTAPSVVRAQPGWEEHLGE
                     VLGKRFVLADANRLRVQVAHPMDNGLVGGIPEFFLNQAQLRDRSGNTLASLELFPAVS
                     ENPTISLDVKGSVETELWLRDNNGNEFKAEL"
     gene            30676..30993
                     /locus_tag="VFF22_20690"
     CDS             30676..30993
                     /locus_tag="VFF22_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004880969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HZX19272.1"
                     /translation="MIKVSVLYPNTPGVRFDMTYYCEQHMAIVRQCLGSACQGIAVDA
                     GLASATPGLPAPFIAMGHLYFDSVAVFEQSFAPHAERIMGDLPNFTDSQPVVQISEVR
                     LAP"
     gene            31028..31957
                     /locus_tag="VFF22_20695"
     CDS             31028..31957
                     /locus_tag="VFF22_20695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     1"
                     /protein_id="HZX19273.1"
                     /translation="MRWILLLAALASACVHANPFTYDLQPRQIADGVWLFEGSTDNFS
                     QANGGNIVNTGFIVTADGVLVIDNGPSRRYGEAMRQAIARVTDKPLLKVMLTHHHPDH
                     VLGNQAFSDVPIAALPGTRDLLAKQGDSMAENMYRLVGDWMRGTEVLLPNEALQAGRL
                     EVGGRPLQLLALSGHTGADLAILDVQSGVLFAGDLVFYQRALTTPGTPGLDTWLADLD
                     QLQQLPWKQIVPGHGPLASDNAPFVQMRDYLGWLDKLLRGGAEQGAEMNELIRTPIPE
                     RFARVSLSRYELIRSVSHLYPRYEQAAMQRVDR"
BASE COUNT         6038 a         9272 c        10082 g         6592 t
ORIGIN      
        1 aggttctcga catccaggct gatgccgtag ccgatgataa acagcagcaa caggctgccg
       61 agcaacgcca tgctgccgcg aatcgggtcg cggcgcagtt ccagggcttc ccggcgggta
      121 taactgaaca gacgcagcag gctgaagcgc ttgcgtttca gcgtattggt ttgcgctgct
      181 ggagccgccg cttctggtgc gggctgttgt gctttttcgc cgacggcatc ctgcaggtag
      241 gcgatgaagg tcgcctccag ggttggcagg ccgcgcttgg ccatcagggc cttgggcgta
      301 tcgctgtcga gcactttgcc ggcgtgcatc aaggagatgc ggtcgcaacg cagggcttcg
      361 ttcataaagt gggtggagat aaatatcgtc acgccgtcat tacgcgaaag gtcgagcatc
      421 atctgccaga acacatcacg ggcaattgga tcgacgcctg atgtcggctc atcaaggatc
      481 agaatttctg gcttatggat gaccgctaca gccagtgaaa gacgctgacg aatacccagc
      541 ggcaggcgct cgggcagctg ttccataacg tcgccgaggc tgaaacgctc agccatggca
      601 gtcacccgac ctgctatctc cgtggccggc acgtgaaaca gctgggcgtg caacaccagg
      661 ttctgccgca cactcagctc ggcatacagc gagaatgact gcgacatata gccgacccgc
      721 ttgcgcgtgg ccatgtcgct ggcattgacc acctgaccga acagcagggc ttgcccttcg
      781 ctggcgggca gcaagccggt gagcatcttc atggtggtgg ttttgccgca gccattagag
      841 ccgagaaagc cgaaaatctc cccgcgctcg atgcggaagt cgacgtggtc gaccgcaaca
      901 aaatcgccga agcgcatgct caggtcgcgc gcctcgatgg caatttcctg ctcgcctgcc
      961 gggcgtggcg ggatcaccag ttgcttatgg ccctggcgtt tagcttccgg catcagggcg
     1021 ataaacgcct cttccagact tgaacattga gtgcgttgca gcagctcggc cgggctgccg
     1081 gtggccaata cggcgccgtc atcgatggcc agcaaatggt cgaagcgctc ggcctcctcc
     1141 atgtacgcag tggcgaccag cacactcatc tgcgggcgct ggcggcgaat acgctcgatc
     1201 aactcccaga actggttacg cgacagcgga tcgacgccag tggtgggctc gtcgaggatc
     1261 agcaggtcag ggtcgtggat cagtgcgcaa cacagcccaa gtttttgctt catgccgccg
     1321 gacagtttgc ccgccggccg ctcaccaaac tcgctaaggc ccgtactgcg cagcagttcg
     1381 gcaatacggg ttttacgttc tgcctcggcc tggccaaaca ggcgggcaaa gaagtccagg
     1441 ttctcgatca ctgagagcgt cggatagagg ttcttaccca gcccctgagg catataggcg
     1501 atatgcggac agacttgccg gcggtgagcg cggctggcca tatcgccgcc gagcacctcg
     1561 atctgcccac tctggatttt gcgtgcgccg gcaatcagcg ccagcaggct ggatttgccc
     1621 acaccgtccg ggccgatcaa gccaaccatg cactgcgcgg gaatttccag gctgacggct
     1681 ttgagggcgc aggttttgcc gtactgcagg ctgacatcct gcagctgcgc gactggcact
     1741 gcaggcgcgc tcactgcgga accttgatcg ccagttctgc aggccactcg gcctgtgggt
     1801 caagccgcag ataagccacg ccgggtacgc cggttttcac ttgggtgagg tggcgctgca
     1861 acagttctgg atcaatccgc gccttgaccc ggaacatcag cttttcgcgc tcgctggcgg
     1921 tttctacggt tttcggggtg aactgggcga cgctggccac gtagctgacc tcggccggaa
     1981 ttacatattg cggtacggca tcgatcacca ggcgaacttc ggcacccagc gctactcggc
     2041 cggcctgctg ctcaggcaga aaaaaggtca tgtagacatc gctcaggtcg accaggttaa
     2101 gcaccttacc gcccgcgccg atcacctcac ccggctcggc gatgcggtac tgcacacgcc
     2161 ctgcgcgcgg tgccttgagc tgactgtcct cgatatcgat ctgtaaacgc tcgacgctgg
     2221 cttgggttgc ctccacagcc gactgcgcct caatcacctg ggactgtgcg gcggcaatgc
     2281 ccgcctcagc cgaaataacc tgggactgtg cggccgccag agcggcttgg gtgctttgca
     2341 acctggcaag gtcgtcatcc agttgctgct gcgccatggc attgcgtttg accaggatcg
     2401 cgctgcgggc gtggcgcttt tgcgcggccg atacctccgc ctggcgctgg cgcacgatgg
     2461 cctgcgcggt gactttctca ctctcgcgca ggcgaacctg ggcactggcc gtcagtttgc
     2521 tgttctgggc ttggcgcatt tgcgcctggg cttgcagcac ttgcgcctgg agtacctggg
     2581 tgtccatacg cgcaatcact tgcccggcct cgacgaactc gccctcatca acctcgatac
     2641 tggcgatgcg ccccggtagc ttactggccg cgtcaacgtc ggtggcttca atccgcccgt
     2701 taccgctggc gaagccatcc cccaggccct cggggcgcag tgtgtaccag agcaggccgg
     2761 cgataattgc cacgacggca acgctggcgg caggaaagat ccattttcga ctgactgaca
     2821 taatgacctt gagtctctaa acaaaggaat tgggtactgc agtactcggc gtcacaccgc
     2881 gcgggctact gctgctcgaa gcggctcaag cgacgccgca ggtggctgtt ttcggcgcgc
     2941 aggtgctcca cttcatcaag caactgcaac gccagggcca cacccgacca ctcaagttgc
     3001 agttcaacct gtaagcgcaa cgcccgcttg gcgacggaaa gtgcatggct actaaaccgc
     3061 cattgctccg gcgtagcacc gcagggttcg acgatgccgt gctcgacaat ctccagcaga
     3121 accgactgcg gcagctcagc gcacaggcag aactcgtgaa tgtctaattg caggatctgg
     3181 ctactcatca ctggctccac tgcgcgcgcg gattgaaggc ggcttgctcg gccagctgtg
     3241 tccatagcgc acgcacaggc tcgtcggctt gagccgggat gacgatcttg aacaatgcat
     3301 acaggtcacc cggctcgccg gctttgttgc gcagcccctt gcctttgatc cgcagccgtt
     3361 gccctgcttg ggtattggcg ggtatgcgca ggttgatttt gccgctcagc gtcggcagcg
     3421 taacttggct gcccagcgcg gcttcccagg gcgccaatgg cacggtgagg accaggttgt
     3481 gcccctcaac atcgaacaat gggtggggca caaagtgaat gatcaaatac aaatcgccat
     3541 gtgcgccgcc acctacaccc ggcgcgcctt gccctttcag gcgaatgcgt tcgccattgc
     3601 tgaccccagg cgggattttt acattcaagg tcttgctgat agccggcaga ggttgaccgt
     3661 cagcgctgta ttgcgggagt tggaaggcga tggtcttggt ttcatctgcc agggtttctt
     3721 ccagcagcag cggcagcgcc atttcaacgt cctgacctct acgcacgcgg ttgcggcctg
     3781 cgtcctggaa acgtccagcg ccaccaaaca gcgactcgaa aaagtcagag tactcactgc
     3841 cagcgctgtt accaccggta aaccccgcgt gacctgacgg ttgccagccc ggtggcggct
     3901 cgaaggggcg gccttgctcg gcgtatttgc gcagctcatc gtactcggcg cgcttttcgg
     3961 cgcttttcaa cacctcatag gcttcggcga cttccttgaa tttattttcc gcatccgctt
     4021 ccttgctgac gtctgggtgg tatttgcgcg ccagtttgcg gtaagccgtc ttcacggctt
     4081 tgtcatcagc ccccggttca agcccgagca ccttgtagta atccttgaag tccatcgagc
     4141 caccttgggt cgtctaccga cacgttgctt acaccagtaa acgctgttca ggtgcgagag
     4201 gttttgtcag ggatcaagaa aactggcatt gcacgtttac agcttcggaa tcgctgttgt
     4261 ctgactgact taggtactga cgagcattac atcgaattgc ccgagcgaac tgatttcaag
     4321 atgcggtctg agtcgcccca gataccttag aaatatcgcg agtgtatgct tctggtcggt
     4381 tttcgtgact gggttctacc ccatttccgc tgttctttgc cgagcaactg ttatgtcgag
     4441 cccgcttacc ttaatcatgc cgactcttga ttaggctgtg tcaaaaggct gcatgggata
     4501 accttgctcc acatttcaac tgaagctgcg aactaaacga tgggttttga acaactagct
     4561 gagttacgcg accgcctgcg ggccgaaaac gttcaggtaa aaactgatag tccgaagcaa
     4621 cgtacgcgaa aatcctctcc gcagaagaag ctgcatgagc aagatcctgc ggtagaagcg
     4681 atctggctat tgcagaagca cttcccatta gcctttcctg tgagtccggc ccccaaggtt
     4741 ccgctaaaag aaggcattct caaagatgct gagcaacacc tagagctgct tggaataacc
     4801 agtgaacagc tcaagcaggg catcgctacc tggtgtcgag gaagtcgata ctgggcaagc
     4861 atgacggaga atgcgccgcg tttggactta accggccagg cagttggcat tgtgacggtg
     4921 gctcaggctc tgcatgcgaa gcatcaggct ctgcggcagc gcggacaagc tcgacggaat
     4981 cagacaaagc cgaaagcgct tttacaggaa aaggctgtcg atactcctgt ggaacagatc
     5041 gtggattgac ggatcactcg cgctcggcac caagtcgctc aatcactgac acggtgcgcg
     5101 aggcgatgat aaggcgactc gaaaagctgc ctgtactgcc tttcagatat gcccgcagta
     5161 agccaaaggc tgccgccaga aatatcaaaa cgcgtactca ccacgattgc ttattggtcg
     5221 ctagcaggac gttaactcgc agtcggatag ttcgcggcta aacccactga tgtggctact
     5281 ggagcttctt tgggcagctt cagggttaag acgatgagca atacccccac gccgctaatg
     5341 gcggcgtaca ccccagcaaa gctgccgctg tgattggcca acagtcctgc cccgtaattg
     5401 ccgagcatac cgccagagcc ttgcatcacg ttggcgaccc caaatatgga tacggccatc
     5461 gcgctggatg atgccagctt agaaacgtaa gcggggataa ggccgaagat tgggtaaaag
     5521 gctgtagaga acagccccgc agcgattaat ggccaataac ccgaaggatg tattacgaag
     5581 atcagtgccg ccgttaccac gcagccatac accagcaaca tggcagcgcg taacccagtg
     5641 cgatctgaga gccaaccgac tgccagcccc gcaaacatgc caacgaagcc gatcacagcc
     5701 cagacctggg cgctgaactg cacgtcaaaa cccagctcag ttcgcaggta ggacgaaagg
     5761 taggtttgga acggaaaggt ggcaaaccct atcaagaaat tcatcgacca gatcagtagt
     5821 acccagggct ttattagcgt taagcgcctg tttggcaccg tcagttcgac gtctgttggt
     5881 gatatggctg gagtcacggt tgggctgagc aaaccacctt tcttgaacac caccagcacc
     5941 aaaagagcca tggctaccgt cagtaggcct acccaccacc agacgctgcg ccattcccct
     6001 tgcgcggcat ataagggcac cagcaggctg ttgattgcga ccccatagct cgtcccgctt
     6061 gataccaggc ccatggccaa accccggtag cggtaggctg taactctggc gacgacatcg
     6121 accattggga caaagaccgt cgcagccgtg cccgccagca gggtaagtaa tgcaccgata
     6181 accaggatgt tgtttgacag ggggatcagc attaacgcaa gcgcgcagac cgccccggat
     6241 gccacgatca tccagccgcc gccgattttg ggcgtcagcc agacggccag cagggcgcag
     6301 attagaaaac caagctgcgc cgaggccgtg atactgccca cgtacgagaa gtcgaaaccc
     6361 agagatgcct gcatgtcggg cacgagctgg gcaaacaggt aaattccgaa gccataggtc
     6421 gcggccacca ggctggttag caacaccaca atcgcagcat tcaaacgcat ggtcagtacc
     6481 gtatgagcaa ttgggattgg cagcgttcag tcagcaaacg ggtgcaacaa cacggcacga
     6541 gtggcgcagg cgtatctatg cgatacggca acctgattgg gcggccatct ttgagtaaat
     6601 gtgcggggtt ctgcttttac tcacgcaaag tcagcggtgc tgagttttcc gttcgctgct
     6661 gcgaatgact ctactgggcg ggcagtccgg acggctgata aaagcgtcct gcagatgttc
     6721 tatagcgcca tgctgaatgc ttatttgcgg ttcaagcagg ctctcacatt gtctcgcata
     6781 gggactctgt agagatggga aggaggacgc aatggtcctg aatgcctcag gggcttttgc
     6841 gggaaaacgt tgtaaatcgt tagatcgcgc ggggcatcgc tgtgccgttc aaaccccggc
     6901 gccggttgaa gcccgcccgt ttacatgcgc cgcctagctc aagcaccagg acgtagctct
     6961 tgtcggttat taattacttc caaagaacta ttgtgcctca gcttgatgcg gtcaatgctg
     7021 gttcttcaac tgcgttctac ggctttgtcc gttatgcccg atgtactcga ttgttcctcc
     7081 agtcagacta taagccctgt ttgcttgcgc ctggataacg gtttgtctgt caggttgttc
     7141 agccagcctt ggttgagcca ggtggcgctg tgtttgcggg tatatgccgg tagccatgat
     7201 gagccgctgg cttacccagg gctggctcat ttcctggagc atctgctgtt tcttggcagc
     7261 gtcgggttta ccggtgagca gcgtctgatg ccttttgtac aggcgtgcgg cggtcaggtg
     7321 aatgcgacca cgcaggcacg ttacaccgag tatttctgtg aggtgccggc cgaatgcctg
     7381 gaaggcgctc tggagcggtt gctggacatg ctggctcggc cgctgctggg agaggtcgag
     7441 caggtgcgcg agcgagaagt ggtgcatgcc gaattcatcg cccgcagcca ggatgtcgac
     7501 accttgcttg gcgctgcgat gggtcaggcc ctggccgacg ggcatcgttg tggtgtgttt
     7561 caggcaggca atcgtgacac cttaccggtc acagatgcgg ccttccaaca ggcgcttagg
     7621 gcttttcacc aggcgttcta tcacgccggc aattgccagt tgagcatcgt tgcaccgtcg
     7681 tcactggagc aacttaatgc cctggtgcaa cgccataggc aactgctgcc cgaagcgcgg
     7741 gtgttcgtcc gtagtcgggt tgcgccgctg ttgccgctgc gcgccgaacg gctgcggctg
     7801 aacctgccgg gcgtagccgc cagtctgcac gtggggtttg cgctggaatt gcctgaggct
     7861 cgcgcggaca gcgccttgga ctggttgctc aactggttgc tggatgaagc ccctgcgggc
     7921 ttgaaggccg gcttgcgtga gcaaggcttg ggcgagctgt tgcgggccca ggtgctgtat
     7981 tgccacgctg ggcaagcttt gttgctgttg agtgtgcatg gtgttgctgc tccggatgag
     8041 gcggtggcgt tactgctcga ctggctggcg tttgtcagtg cgcgggccga cctcgtggct
     8101 tggcatgatg cgtatcagca gatttgccaa cagcgtctgt ttggcatgac gccactgacg
     8161 ttggcccgta attggcaagc ccccgagcac aaggagggtg gggcgatgcg catgctgttg
     8221 cagcaattgc agcagccgca gaggcgttgt gtactgctgg cggataaccg cgagttgcca
     8281 cactggcccg gcgccggttt ccccctgcaa atgcgtgccg agccggcgtc gtctgtgcca
     8341 gttttgcgtg gccgttggca gttgcctgca cgcaatccct tgctggcaga ggctgaggtg
     8401 ccgggagtga gactggccgt acccgctgcg tttcgctggc tagcggcgcc agttcattac
     8461 gcggtacctg cggccgctgt cgcagtgtgg caggcgcgtt gctgttttgt tgagccactg
     8521 ggggcgaaca ttttgctcaa cctggcgcat acaggcttgc gggccgtgcg ccagcacctg
     8581 cagcacgtgg gtatcagcct gagtctggaa gcggagacag ccagcttgag tgttcgtttg
     8641 cagggcagtg ccaggttgat tccgggggcg gttgtcttgt tgatgccggc gctcttgcag
     8701 cctcaccctg cgcagtggtc cgcactggcg gtcgctgagc cgccgagcat gccgattcgc
     8761 cagctgttgg ccgcgtctgc cgagctgttg caggggcaag ctattgatct tgcggacgag
     8821 gcggttgcca cgttgcaacg gcgttatcgc caggtgcgta ttgaagccct gggtgtgggg
     8881 ctggatcgcc cggggcaggc gctaatcgaa agcctattcc gcacctccgc gccttggccg
     8941 tctgtggcca tcgcgccgtt acagcctggg ttgcactggc gaacacgggc agcgcacggg
     9001 gacagtgcat tgctgctgtt ctgcccgcaa gtggatgact cgcccgccac tgaggcctgc
     9061 tggcggctgt tggcgcatct gcttcagggc gcgttctacc agcgcctgcg cagtgaattg
     9121 cagctgggtt atgcggtgtt ctgcagttac cggcagatcc aggggcgacg cggtgtgctg
     9181 ttcggtgtgc aatcaccggg gtgcaaggct gcagagatcc tgcagcacat tcaggttttt
     9241 ctgcaggcgc gctgtacctg gctggcaggg gtggatcagg ctgcattgat tgcggcactt
     9301 accgcactgc gtggtcaatg gcaggcgcag gcgcgcagct gtgacgggct ggccgagcag
     9361 gcctggctgg cgcatcttgc cggtctttca gccgcgcatg gcgaggccgt gcaacaggcg
     9421 ttgcagcagc tcagcctaga gaccgtacag caggctctgc aggcactgct tgaggcctcc
     9481 tcaggctggt acgtgctgag caacgaggca gcgcccggcc acgcctgagg gctggcgctg
     9541 ttgcccacga ctttccgttc tctggggtta cgagcccctt tggttcagcc catcggtggg
     9601 ctggcccctg cgcatcaatg gttttctcat ccgtaccaat accgccgctg ttcgcctgag
     9661 cagggattag tccgtctacg accttgggcg gtcatccttg cggtcagggc ctttgggaga
     9721 ggcggtcgta caccgttccg gcgctgcttc caggacacgc caaagcagca gccaatcaag
     9781 gccatggcag cgtatggcgg ctgtcagggc ttttcgataa tcgcctccga cccgactgac
     9841 cgttgccggt cgtcccatcc agcggagagt gttatgaaca acaacaagat cgccagcacc
     9901 cgatttctgc ccctgaccct ggctgccgcc gtcgccatgg tgacagcgca acatgcagct
     9961 gccgagatcg tcctgtatga caaggacgac accacctttt ccaccgatgg ctatatcaac
    10021 gccttctatg tgaacagcga tgtcgaccgt gagggcgagc agttcgaccg ccgccagtcg
    10081 cgggtgaaga tgggctttct gcccaactgg atcggcttca atttcggcaa gcagatcgat
    10141 gacctgaagc tcggcgggcg ttcgtcgttc tgggtcacca tcaacgacag tgaaagcaac
    10201 ggcaccgata ccgccatcga cgtgcgccag ttctacggca ccgtggcatc gccagagtgg
    10261 ggcgaagtgc tggtgggtaa ggacttcggc ctgttctcac gctcgaatat cttccttgat
    10321 gaactgttgg ccggttacgg caacgtcagc gacaccctgg gcctggtgga tggcaccggc
    10381 gtatcgttcg gcaatatcgg taccggctac ccgtacccgt tcccgacgtc gcagatcacc
    10441 taccgcaaca acaacctggc agaaggccta cgcgtggcag ttggcatcat ggacccggtg
    10501 gacaccaacg atgacagcgc tgtgggcaag tcctaccagg aagagccgcg cttcgagtcc
    10561 gaggtcagct accagttcga tgttggtggc gcgaccatct attcctggct caacggcgct
    10621 catcagacct ccgataacac cgacaaggcc gtcgacagcg tgacctcgca aggcttgggt
    10681 tatggcgtgc aggcgaagat ggctggcttc tcggtcactg cgtcgggctt ccaggctgaa
    10741 gggatcaacc cgttcttcac caacaacgcc ggtgaagcca cgctgcgcga gatcgacagc
    10801 gaaggctacc tgctgcaggg ctcctacacc ttcggcaaga accgcgtggc gctgtcctac
    10861 ggcaagaccg aggatgatgg taacggcctg ggcactgccg ccgattatga gacccgcggc
    10921 gtggcttact tccgcaccat caatgacaac ctcaagctgg tggccgagta caaccagtac
    10981 gagatcgatg ccgccgaggg cagcggtctg ggtgaggaca ccaacacctt cgcggtgggt
    11041 gccgtcctca gctggtaagc atgcagcaca ggccgggcag accttgcccg gcttttacct
    11101 ggaggttgcc atgcccctgg atgcactgct gattctgctg ccctgcagca gcgtactgct
    11161 cgcattgcca ctcgtgctaa tgcgttgcct gtaaccctta cctgatacca aggacgacac
    11221 catgctcggt ttatggagtg cgcatgccca gcaatacctg ctgtggctga ccctggccac
    11281 caccctggtc tttgcgttac cgattttctg catcccgctg gtctgggccc gtctgatggg
    11341 ctggacgctg cccagccaca ccgacctggc ggtgtacttc gggcgctgcc tgggcgcctt
    11401 tattctgatt ctcgaagcca tgatgctgcg cgccgcattg agcggtgagg cgattcgcgc
    11461 ggtgttcgag atgctcctgg cagtggccgt gatgatgatc atcgtccacg tctacggtgc
    11521 cctgcggcgc attcagccgc tcagcgaaac cctggagatc ggcttctaca ctggcctgct
    11581 ggtgctgaca ctgttgttct ggcccgctac ttaggaacta gtcctgcgct actcaagtgg
    11641 catgtggggc ggtgggtcag cttcgtagaa taaccccagt aattatcgtg tttctcatgg
    11701 gggctgctat gccgcatacg cttaccgaag gtgtggtgac tgccacctcc aacgccgcgg
    11761 atgtcgagct ggtggcgcag gagttggccc gccagctgat ccatccgcac ctcggcttcg
    11821 tactgttctt ctgctcggca gaatatgacc tgcagggcct gggcgaggcg ctggaccaat
    11881 actttggcgg gattaaccta gtgggctgca ccagtgcggg ggagatcacc gcccaaggtt
    11941 atgaccgtgg ctgcgtcagc gcgatcggtt ttgatcaccg tagtttctcg attgccagtg
    12001 cgctgatcga cgagatggag cgcttcagcc tcatcgatgc acagcagttg gttgaacgtc
    12061 tggtcagtga ttgccgcaac aatgaactgg cggccatcaa gggccacagt ttcgccctaa
    12121 ccttgctcga cggcctgtcc agtcgcgagg aggtggtgct cggcgccctg agcgcggcgt
    12181 ttggcagcat tccgcacttt ggcggttcgg ccggcgatga caatcacctg acccataccc
    12241 acgtctacta cagcggcgag ttccacaccg gggccgccgt ggtggtgcta ttcaatacct
    12301 ggctggattt cgaggtgttc agcacccacc acatcctacc gcgcagcgag aagctggtag
    12361 tgactcgtgc cgacagcacc agtcggcggg tgtatgaact caatgccgca ccggctgcgt
    12421 tggagtatgc cgagttgatc ggggtgccgc ttgccgccct cgaccaccgc ctttttgccg
    12481 cccatccact ggccgtacga attggtggcc agtactacgt gcgatcaatt cagcgggtca
    12541 atgatgatct cagcctgacc ttctactgcg cggtagagaa cggcatcgtg ctcaccgcca
    12601 tgcagcctgg gccactgctg ccggatttgc agggggtatt cgaaggcctg cgtgagcggc
    12661 tcggcccgtt gctgctgacg ataggttgcg actgctttct gcgccgcctg gaaattgaac
    12721 ggcgcgacgg ggtcgagccg gtctcggcct ttctgcgcag ccagaacgtg atcggcttca
    12781 acacctacgg agagcagttc aatggtatgc acatcaacca gaccttcacc ggagtcgcca
    12841 ttggccgtcc cggtcgcggc tgatgttgcc gagctgcaag cccgttgcct ggaactggaa
    12901 cggcaaaacg gcaaactccg gcagatcaac agcgcgctaa tcgagcgagt cgagtcggtg
    12961 cattcacgtg gtaacgatgg ctacgccgcg ttccagcatt cggtggtgct ggccgagcag
    13021 gtgcgtgagc gcaccgatgc cctcaaccag gccatggccg aactgaagtc cagcaaccag
    13081 ttgctcagtg acgcgcgtct gcgtgcggag acggcccacc agcacctgat tgatgccatc
    13141 gaaagtattt ccgatgcctt cgtgctgttt gatcgtgagc agcgcatcgt actgtttaac
    13201 agccgcttca agtcgttctg ggctggcagc cgggcacgga tcagcgccgg cactcgcctg
    13261 gccgaaatca agcgcctgag caaaagcacc ggcctggtgg ttgaagagca gcgcagtaac
    13321 cgcgacgaca acatgctcta tcgtctgcag gatggacgct gggtacaggt cagcgagcgg
    13381 cctacccgcg agggcgggct agtgatcctc tacaccgata tcaccgaggt caaactcaac
    13441 gaaactatgc gccgcgagca ggccttagcg cagaagtcgc ggttgttgca acgggccatg
    13501 gataacctgt cccagggcat ggccatggtc aatgcagacg gtcagctgga attgtggaat
    13561 caccgttttc tcgacctctg cggtttggct ccaatcagtg ctcaccggcc gtttgccgag
    13621 gtaatggctg agagcgagct ggaactgctg acgcccgcca gccgcgatgc caacggccgg
    13681 ttgatcgagg aactggagtt gtgcctgttt gacggacgca tgctggaggt gcgcacccat
    13741 ccgctaccca caggtggctt cgttaatacc ttcactgaca tcaccgagcg acaccgtcat
    13801 gccaaggctc tgagcgaaag cgaacgctgg attcgcctga tcaccgacca tgtgccggcg
    13861 ctgatcgcct acctcacggc ggatctggtc taccagttca ccaacaaggt ctacgaggaa
    13921 tggtactgct ggcctcgtag cgcgatgctt gggcaaagcc tgcgggtggt gcacagcgag
    13981 gagcattgcc ggcgcttgga gccgtttatc gagcgggcgt tatctggcga aagcgtgacc
    14041 ttcgaattcg ctgaaaccaa ccaccatggc caggagcgct acatgctgcg ttcctacgtg
    14101 cccaaccgcc aggccagtgg tgaggttgtc ggcatcttcg tgctgatccg cgatatcacc
    14161 gagcgtcggc gcactgccga ggccctgcac caggcctacc agcacctgga gcagcgggtg
    14221 cgtgaacgta ctgccgagct gaccgagctg aatagccagc tgcgccgcga gatcgatgag
    14281 cgcacgcaga tggaagcacg cctgcgtgag gccaaaggcg aggcggaaca ggccaacctg
    14341 tcgaagacca aattcctcgc cgccgtcagc cacgacctgt tgcagccgct caatgcggcg
    14401 cggctgttta ccagtgcgct actggaaaag cctgacctgg ccgacagtgc gaccctggtg
    14461 cgcaatgtca gcaactcgct ggaggatgtg gaaagcctgc tcggcaccct ggtggatatt
    14521 tctaagcttg acgccggggt gatcaagccc gatatcgcgc cgttcgcggt cggcgaactg
    14581 ctggaaaacc tcgccgccga ataccaccag atcgccggca gcgaaggcct gcgcctggac
    14641 ttcatcccca gctcagcact ggtgcgcagt gatattcagc tacttgctcg catcctgcgc
    14701 aacctgctca gcaatgccat tcgctacacc tctagcggac gtatcctgct gggctgccgg
    14761 cggcgtcggc aaagcctatc gatcgaggtc tgggacaccg gcattggcat cgccgatgac
    14821 aagctcggcg agatcttcca agagttcaaa cgcggtgacg tcagccaccg caagcaggac
    14881 cgcggcctgg gcctggggct ggcaattgtc gagaagattg cccgcatgct cggccaccgc
    14941 atccgcgtgg cctcgcgcca gggaaaaggc tcgatgtttg ccatcgaagt gccgctgacc
    15001 agtcttgcgc cgcgaacccg cgcggtgatg gatgcgccca atccgctggt agagcggctt
    15061 agcggttcac gcatctgggt gctggataac gatgcggcga tctgcgcagg catgcgcacg
    15121 ctgctcgaaa cctggggctg ccaggtggtc acggcattgt ccgaagagga cctggcgcgc
    15181 caggtcgata acttccacgc tgatgccgac ctgctgatcg ccgattacca ccttgatgat
    15241 gaccacaacg gcgtggatgc cgtggcacag atcaatgccc gacgtagctc acccttgcct
    15301 gcgctgatga tcaccgccaa ctacagcaac gaactcaagc aacagatgcg cgaactgggc
    15361 cacaccctga tgcacaaacc agtgcggccg atgaagctga aaaccgcgat gagtcatttg
    15421 ctggagcggc gggagtagcc ggtgcgcgtg gcgcacccta cgccagtaag acgcccgtcg
    15481 accgtagggt gcgccgcgcg caccgtcatt cgcccaaatc accctcatca gaccttccac
    15541 ctccccagtc ggctggataa aggccctgcc ttaccaggcg gtggaagctg gaccatggcc
    15601 agtcagccac ccggttgacc aggccatgtt tgaccgggtt ccagtgcaca tagtccagat
    15661 gccgctggta atcctgcgtg tctcggatct ggtgttccca gaagcgtcgt tgccacagcc
    15721 ccgattcacg gcgcaggtgc cggctgagac tgacggtgcc gggtaacgcc aattgttgac
    15781 tggtctgacg cttgatcaac gaccaacgtc ggccatagtc ggtatcgtgt tcgggtaacg
    15841 cccacatgca gtgcaggtga tcgggtagaa gaacccaggc gtggatcgag aagggataac
    15901 gttggcgcac ctgttcgatg gcctggcgta gggcgatacg cagttcgggt tgggtgagca
    15961 gtggacggcg ttgttcggtg gtcagggtaa agaaatacag ccgaccctcg tgcgcgcggc
    16021 ggtaattgga cattccttgt cctcgtcttg agttgtgtaa cggcattggt gcgcatggcg
    16081 caccctacga aagagcgtag acctgcggcc ggacataggt ataagagtgc gcgccttaca
    16141 cgtagggtgc gccgcgcgca ccaaggtccc ttaacggcgc agataggccg tgaagtcgat
    16201 atccccggcg gagagaatcg cctggactcg gttgtgcacg ttgagcttgc gcaggatggc
    16261 cgaaacatgg gccttcactg tggtttcggc gatgtccagg ctgtaggcga tctgtttgtt
    16321 ggactcaccc ttggtcatgc gttcgagtac cagcaactgc ttgcgggtca gggcctgcag
    16381 cagctccgag gggaagctgt ggtggtcggt gtggctgcgc tgcgagccgc ttttctgcgt
    16441 gcggatgatg tccggtggca ggtagacatt gccattgagg atttgctgga tggcatcagt
    16501 catctgcgcc cgtggtgatg acttagtgat gaagccaacc gcaccgtagg tgatggcctg
    16561 caggacgatc tgcttgtctt gttcggccga gacgatcacc accgggatgg tcggcgcctc
    16621 gttgcgcagg ttgatcaggc cgcctaggcc atgcatgccg ggcatgttca ggtcgagcag
    16681 gatcaggtct aggtcgtcat gctcttgggt cagtgccagg gcgctgtcga gatcggcggt
    16741 ttccatgatg ttgctgcctg ggaagccgtc gctgatgacg ttgtggatgg cttcgcgaaa
    16801 cagcgggtgg tcatcggcaa tcagaatttt gtacatggcc ttatcacctc gttgttttga
    16861 ttatggtggg gttgcagccg taacgcatgg tttaacgcag caagggtaga ggttgtgccg
    16921 gtatcagggg cagaccggcc tgttgccagg catcgatgcc gtcacggtac cagtacaggt
    16981 tggtgtaacc cagatgatgg gcgcgtttta ctgcattcca acccagccag cagtcggagc
    17041 ggcagtagaa caccaagggc caggcgcgat tgccctggct ggcctgggtc aggtgctggt
    17101 ggaagtaggt ctgccactgc gcgttcagct caccgtcacc cgtgttggct agccacaggc
    17161 tgtttggcag gttggcgtgg gtttcgtctt cgatgaactg gccatgcagc cattgcctgc
    17221 gatagacatc gatcagtcgg gtgccgggct gttcgcgcag taactgttgc agggcggcgg
    17281 tgtccaggat gcgtgcgtgt tcgctggtca ggggtgtggg gctacggtaa tggctcagac
    17341 ggtagccatc ggcagagaac aggggagtat cggccagtac agggctggtc ggtaacaggc
    17401 tggcaatcaa tatcagggcc tttggccagt gtttcatcgc ctttcttctc ggttcttttt
    17461 attggcctat tacagggcaa gaccgatgct ttgggaattc tacgttggag aggaaaacca
    17521 gtaccaaagt agtagatcgg ccaatgtccc tgttgtgcca cgcgcaagac cgcgagctag
    17581 ccattaccct tgcgcagcgc cgcgtgctga gggttgaagg tcagcaccgc gagcacggcg
    17641 aatagcaggg tcaggcccag gcacacggcc agtgccagcg ggctgaagcg ctggtagagg
    17701 gcaaagcgca ccagctccac ggcatgggta aaggggttga gggcgcacag ccagtacagc
    17761 cactcgctgg cctcgcgcat cttccataac ggataaagcg ccgacgacag aaagaacagc
    17821 gggaagatga cgaaattcat caccccggcg aagttctcca gttggcgtat gccatttgac
    17881 agcaataagc cgagcgcgct gagcaatagt gccaccagca gcagggcggg cagggcactg
    17941 agcaggcccc acagcggcgg ttgcacgcca tagacccagg caatggcgag aaaggcgtag
    18001 acctgcagca gcgagatcag ggcaatcgcc aatagtttgc cggctagcag aaaggcgcgt
    18061 ggcagtgggc tggtgagcag tacgcgcatg ctgcccatct cgcggtcgta gaccatcgac
    18121 agtgagccct gcatgccatt gaacagcagg atcatgcagg ccagtcccgg cacgatgtag
    18181 gtttcgtagg tgatgtaggt gtcgtagggc tcgatgatgg cgatgccgag tgccgcgcga
    18241 aaaccggcgg cgaataccag cagccacagc aacggccgca ccagcgcgct gaggaagcgc
    18301 gagcgttgca gtacgaagcg cagccactcg cgctgcacga tgccttgtag gcaataccag
    18361 taagtgttca tcgacaactc tccgtagggt gcgccgtgcg cacctgattc ttgcctgagt
    18421 tcatggcggt acgcacggcg caccctacgc gcatggcgtg gttaggcggg cgaagctggc
    18481 ggccagatca tggccaaagt cttcggcctg gccctgggcg accagacggc tgtggttgag
    18541 gatcagtagg tcgtcgcctg gctcgatctc atcgagcaaa tgggtggtcc agagcacact
    18601 caagccttca tcgcggctca gctgacggac atgctgattc agcgccaggc gactggccgg
    18661 gtcgaggccg gcgctggcct cgtcgagtag caacaggcgt gggcgatgca gcagtgaacg
    18721 ggcaatctcc acacgtcggc ggtgaccgcc gttgagggcg cggatcttct cgtggcggcg
    18781 ctctgtcaaa gtgtggcgtt caagttcttc atcaatgcgc gcctgtgccg tgcgcttact
    18841 aacgccgtgt agggctgcgt ggtaggccag gttttgctgc acggagaggt cgaggtccaa
    18901 ggtgctttgc tggaatacca cgcccagctg gcgcagcgcc ggacgcgggg tggtacgcag
    18961 gctatggccg aagatatgga tgtcgccgtg ctgcaggtca taaaggcggg tgagcagagc
    19021 gatcagcgtc gacttgccgg cgccgttggg ccccagcagc gcggcgaagc ggcccgtagt
    19081 caggctgaag gcaacctcct gcaacgccgg gcgtgacccg taggcgaagc tgacgccctc
    19141 cagctgcagg gcattcatgg cgtgaccacc acgccccagg ggtagcggcc gaccttgatc
    19201 gacttgctga ccttgagcgt atcgacatca atcactgaga catcgccact gaccccgttg
    19261 gtggtcagca gacgtttttg gtccggggta aaggccaggt gccagacccg tcggccgacc
    19321 agcaggtagt cgagcacttc gtagctcttg gcatcgacca ctgccacatg gttggccggg
    19381 ccgagggcga caaaagcgta cttgccgtcg tcactcagtt tgactcccac cggctgcacc
    19441 ttgtccgggt gcacgccttt gatctggaag gtcagcacct tctcgatctg ccggctggcc
    19501 acatcaatca cactcaccgt gccgccgatc tcagccgagg cccataggcg tttgccgttc
    19561 ttgtcgaatt ccacgtgccg tgggcgctga tcgaccagcg tgttgtccac cagttgctgg
    19621 gtcgtggtgt cgatccagtg cagcatgttg gtggtttcgc tggtgttgac cgcccatttg
    19681 ccatcggggc tgacggccat gccttcgggc tcaacgccga catcaatttg ccccagcacc
    19741 tcgtcgctct gggtgtctac cacggtaacc agcgcatcgt cctcgttgga gatgtacagc
    19801 cagcggtcgt tggggtgcag ggcgaattgt tcggggtcgg cgccggaggg cagctccttg
    19861 atgatcgtgc gcgtggccag gtccatcacc tgcacgcggt ccgagtcgct ggcgcagatg
    19921 tacagcagct tgttatcgga ggacagcagc aatccgcggg ggcgcatgcc cacatcaagg
    19981 gttgcggtga cttcgaggct gccgaggtcg atgacgctga tgctgtcatc cttctcgttg
    20041 gacacatagg cggtggtggc cagggcagtg tggctggcca ggccaagggc gatggcgcag
    20101 gcaaggtgag tcagacgcat ggggttgatc ctcttgttgt tatcggtggt gtggtggttg
    20161 gtgcgcacgg cgcaccctac ggcggtgggt gggttacggg ctgtagggtg tgccttgccc
    20221 accgcgggtt aggtcacagc tcacctccgc ctggtcatac cctaggctgt ccatctcgtt
    20281 gtgcggatgc agaaaaccgt cctgcggcga ggtgctgacc agggcgcgtg gttgtaccag
    20341 cggaattggc tggcgcagct cgccattcca cggtcgaaag ctcagcttgc ggcccttgaa
    20401 accgtccagc ggcagctcgg cggtcagcgc caggttgcgg atggcctgcg gctcggtcgc
    20461 gcgcagcttg gtcacggcgg cggcgatgct gcgcacggcc atccaggcag cgaaatcgcg
    20521 gtcgttcatc cagcgcccgg ccagcgcctc gaagcgtttc tgcagttgcg cggcgccgaa
    20581 ggtttccacc gtcttgtgcc aggcggtcgg ggtcaggcct tgggtgccgg ccaccgggcg
    20641 cgggtaccag gtgttgtagg gcaggtactc gccgaagtca ccacgctcat cggccaccag
    20701 caccacgtcg tactccgtgg tttgggtgaa cagcggcatt tccgcctgag cgctgcggcg
    20761 ctggtcattg tcgaagctcc aggttttttc cgcgacgatg cgatggccga agcgtttagc
    20821 ggcccgtttg agggcgctgg cataggcctg gtcgtcttcc gtactgccgc tgatcaacag
    20881 ccagcgcgtc catttgcgca ctgcaagaaa ctgcgccagg gcgtcggcca gcatggcgcg
    20941 gctgggtagg gtgtgcagca cattgttcag gcattgacgt tgccgcaggc tgtcatcggc
    21001 gctgccggcg ttgagcagca ggctgtccgg cagcgcttgg ctgagctggc gcaggctgct
    21061 ggccggggca ttgaccacga ataaacgcac gccctgcgcg tgcagctcgc gcgctgccgt
    21121 cagcaggctc gcaccatcgg tatgttcggc cacatcgagc tggtagctgt gcttaagaaa
    21181 gtgcccggtg ctgttgctgt cgctgatcgc cagttcggcg cctcggcggc cggcgtcgct
    21241 cggttcgggg atgacattgg acagcagtgg gccactggcc ggggtgtaac ccaggtaggc
    21301 gatgcgcacc tgcaatacgt ctgcggccgg agtggccagg catggcagcg ccaggctgca
    21361 cagcacggcg gcacacagca tgagtgatgg gttgatccgc tgcatggggc gactcctggc
    21421 taggtgttgc cagcatagga acagcccggt attcgggaaa tatgcccaaa gtaccagtac
    21481 gccagtacca aggtagtaag tcggcgggtg cgccagcgtt ttaggatgat cccatcccat
    21541 aaaacaagaa caggtaccct ccatgagcac gctcaaaaca ctcgccctgg cgctgattct
    21601 gctgagcagc ctgatcggtt ttgacagcat ctatgccgca ggcgacctga ctcggcgcac
    21661 cgagaaattg ccggacctgc tgctgggcag cgaagccagc gactacagcc tgtcgcagag
    21721 cgaatatcag ctggaaaccg gcaaggccta ccagctcaag atcatcgcca gcgggcagaa
    21781 ggaatacgcc tttcaggccc ccgagttcgc cacctcgatc tacctgcgca aggtcgaagc
    21841 tggcggtgtt gagatcaagg cgatcaccct catcgaactg gaattcgagg acgccggcga
    21901 agcggagatc ttcttcgtac cgatcaagcc cggcacgttc cgcttctatg ccaagggcct
    21961 ggaaggcaag ggcatgctcg gtcatttcgt ggtcaagtaa cgtgtccgtt ctgtggcgca
    22021 tctacctgct ggtgacgctg ttcttcgttc tggtcagcgt ggtggggctg gcgctgctgc
    22081 tgcgccaggc ccgcgaagat gtgcagcgtg aggtgcaggc tgccgaggct gtggtggaat
    22141 acttgcacga ggcggcccgg cgcgacccgg ccagcctgca gagcgggctg accggcaacc
    22201 tgcggcatgt ccgcgtacag tggctaccgg cccctgctac aacgccagac gcctcgttcg
    22261 atcagtggct ggggcagcgc ctgtttcccc ttgagcaccg cccgcgccgc gtgcaactgg
    22321 cggatggtcg gctgttgcag attgctgtcg acccgacgga cgagattgat gaggtctggg
    22381 attcgctgct gcagttgctg ctgctctgcc tgctggcgct gctgatcagc ctggtggcga
    22441 ttcgctgggc cgtgcaccgt ggcctgcggg tgctggatga actgctcgga ggcctgcatc
    22501 aggtcactca gggccggctc gacgcgcgtc tgcctgccca cagcttgccg gaagcgcggc
    22561 aattggccgg gcatttcaat gccatggcca ccaccctgca gcaggtgcag gccgacaaca
    22621 ccgaactgac ccaggcgctg atggccctgc aggagcgcga gcgcgcccgc ctcgggcaga
    22681 ccctgcatga tgaccttggt cagtacctca gtggcatccg tgcccaggcc tgcctgttgc
    22741 aggcggtggc cgatcagccc gagcaggtgc gcctcacggc ccagcatctg gacaaccata
    22801 gtcagcgctt gcaagaaggt tttcgcgccc tgatccgcga cctctacccg gtggtgctgg
    22861 agcgcctcga actggatgag gcgctgcaac tgctcgctga caactggcag gccgcgcagc
    22921 gcgtgcaatg ccgggtcatg ctcagccgcc atttgccgag cctgccgctg ccgaccaagg
    22981 cgcatctgta ccggttggtg caggaggctc tgaccaatgt ggcccggcat gcccaggcca
    23041 gcgaagtgcg cgtgcgcctg cattatcagg gccagcgact gcgcctgttt attcgcgaca
    23101 atggccgtgg cgcccagctg ccgcagcgtc cgggtattgg cctgcgttcg attcacgagc
    23161 gtgcgcgcag cctgggcgcc gtgctgcgtg tgcacagccg tcctcacgcc gggtgggcgc
    23221 tgtgcctgac tctggcattg ccggagcccg agtgatgaag atacttctgg tcgatgatca
    23281 tgcggtggtc cgtcagggct atgccagcct gctgcgggcc ttgttgccag acgtgcaggt
    23341 gcgcgaggcg tgcgatggcg agcaggctct gcagatggtc caggaggaaa tccccaacct
    23401 ggtcatcatg gacatcggcc tgcccggcat cagcggcctg gaggccaccc gccggctgcg
    23461 tcagcgcctg ccgcaactgc gcgtgctgtt cttcagcatg cacgacgaac tgccgttggt
    23521 gcgccaggcc ctggatgccg gggcactggg ttacctgacc aagacttcct cgccggaggt
    23581 gctggtcgaa gcggtcaagc gcaccctggc tgggcatgcc tacatcgagc agagcctggc
    23641 cactcacctg gcctgtaacc ccctgggtga gggcagcagt gatccgcgcc tgcgcggcat
    23701 gacccaacgt gaattcgaga tattcgtgat gctggccaaa ggcatgccca gccggcagat
    23761 cgccgagcgg ctgtgcatca gcggcaagac cgtctccaac tacctgaccc tgctcaaaag
    23821 caaactgcag gtcagctccc aggccgagct ggtgcacctg gccatcgaca ccggcgtgat
    23881 ccgggtgggg ctggagagcg cctagggcgg ttgctcagga tattgggagt tagctcagga
    23941 tgagcccatc gtagggtgcg ccgtgcgcac caagaatttg gctatttagc cattgcctgc
    24001 tgtgaggcga taagtcgtag gttggtgttg agcgcagcga agcccaacag acagtggcat
    24061 gtctcgttgg gcttcgtcgc gttgctcctt aacccaacct acacgctgag acggcaaggt
    24121 cgccaggctg caatggcctg aaggcgatac cactttctac ccagccataa tcagtgcggc
    24181 aaaagcacaa ttccctataa cactggccgc ggcttgcggg ctcgccgcac gccgttgatt
    24241 cggtgtagat tgatggcact gggccatggc gggcatgcgg tgttagaccg ggttctcgtt
    24301 taatggatta atgtggaatt ctgtctaatc acggttatac gaattaaata tgagtaatag
    24361 agcaaatttt cttgagccga caaagcaagc tttggcaaaa agagcaggga atagctgctc
    24421 atttcccggg tgcgatgcta ttactgaagg gcccagctcc gagtctgatt caagtgtcag
    24481 taaaactgga atggcatgcc atatctatgc agcggccaat ggtccaagcg ctagacgagt
    24541 caacttgcaa ttgtcggatg cgcaattatc tgaaatctct aatggcatct ggatgtgcta
    24601 ttcgcatgga aagttaattg atactgatga ggctacatat acagtagagc aactaaaaac
    24661 atggaaagtt atttctgaat taagggctag attgtggcag caacttggca aaagaattga
    24721 gttgcctcct gagagcttta aatgtattcc attaccagag accaagattg ggtttgagtc
    24781 attaggtcaa gaaaatatct tgataggcga ggctatttca agagcttgtg ttagccaagt
    24841 ctgggggaaa ccagaaagcc aagccatccg cgacacacta atagaactga caagaaatgc
    24901 atttgggcac ggaaaagcat catgtctctc tatacaaata aaaccaaaat caattgtttt
    24961 aactgatgat ggtacaaaat atgatccgat aaaaattttt actgagaacc atcaaggcgg
    25021 cggctttcat tctatcgatg agttagttaa taatcatgca gggtcagttt atttttcgtt
    25081 ccacaatgat ggaaataaga ataactatat cttctcaata gtgtccagcc caaatgacat
    25141 aaaggaatta acgccttgcc atattgaaat tccaagagag tattactggg aggaaggggt
    25201 aaaatttgaa gtcgctgaat tctgcgacac agtatatgtg atatttccag acttctttgc
    25261 tttaagtgat gctctcaagc tgcctgtttt aatagaggag cacttaccaa aaggcaagga
    25321 ttacgtattc gttggagtag atctatccca aagggcaata gaaataattg aaagtcgtgt
    25381 ggagaacata aaaatactca attatcgcga ctaatttgat cgttcccacg ctctgcgtgg
    25441 gaatgcagcc cacgacgctc cgcgtccact tgcaggctat gcgcacttga atccgctggc
    25501 gacgcagagc gtcgcaagct agctaccacg cggagcgtgg gagctatcta atcaacccgc
    25561 tcccgtattt ccgcagccat tcaatcccac cccttggggc attccacaca gccttccata
    25621 ttggcgccct ggaaattgcc gtagtgggtg cgcgtgccgc tcaggtcggc gttggcgagg
    25681 ttggaaccgg agagtttgac ttcctgcagg ttggcgtcct tgagatcggc gcccttgagg
    25741 tcggccttgc tcagccaggt catttccagg ttggcggcct gcaggttgac cgcgtgcatt
    25801 ttggcggccg aaaggcgggc gaactccagg ttggccgcgc gcatgtcggc gccttcgaat
    25861 tgggcggctt gggcgaacag tgcccagccg ttgatggcca ccagggtgct gttgctgaag
    25921 ttggccaggc gcaggttgct ttgctgcagg ctggcgcggg tcaggtcggc gccttgcagc
    25981 cgggcttttt caaggttggc caggtccagg cggctatggc gcaggtcggc accgcgcagg
    26041 tcggcacctg ccaggttcat gcggcgcatg tcctggttgc tcaggttggc attacgcagg
    26101 tcggcgttgg ggcactggct gtcggcttcg atgcggcagc cgttgatggt tagcacctcg
    26161 tcggcattga ccagttcggt acccaggatc agcagtacgg tgggcagtag taggggcagg
    26221 tagttcatgg ctatggcctc gggtggggtg cgcggggccg tgcaggccat gcgcaccgag
    26281 gtatacgaag tcgggtgcct ggcagagcac ccgaacgaga gaattggtgc gcgcggcgca
    26341 ccctacgggt agggcgcgcc ggcgacctag cgttgcgcgg tcttgttatc ccagcttggc
    26401 agtttgaaca cccagaacga gccgccttgc gcgaccggtt tggtcagctc ggccatatcg
    26461 ccgccccaca gcggtactgc gccgccgtag ccggtggtca cgccgatgta ctgttcgcca
    26521 tcctgttccc aggtgatggg cggggagatg attccggtgc cggtctggaa cttccacagc
    26581 tccttgccgt ttttcgcgtc aaaggctttg aagtagccat cgccggtgcc ggtgaacacc
    26641 aggttgccct tggttgccag cacgccggcc cagagtggca gtttttcctt gtgttcccag
    26701 accaccttgc cggtagtcgg gttcatggca cggaggatgc cgacgtggtc gtcatacagg
    26761 cgcttgatgc gaaagcccat gcccaggtag gcagagccgg ctttgtaggt ggcttcttcg
    26821 gcccagtagt cttctttcca gtggttggcc ggtacgtaga ataggccggt gtcctggctg
    26881 taggccatgg ggttccagtt tttgccgccg aggaagggtg gcgacacttc taccgatttg
    26941 cctttggctt cgcccggctc cggttttgct ggacgctggc cttcgttctc taccggacgc
    27001 ccggttttca ggtcgatgtg gctggcccag gtgatcttgt ctacgaaggg gaaggcgttc
    27061 tgcagcttgc cgttggcccg gtcgacgaca tagaagaagc cattgcggtc cgcgtgggct
    27121 gtggctttga cggttttgcc ggccttgtcc ttgtagtcga acagcaccag ttcgttgttg
    27181 ccggagaagt cccaggcatc gttcggggtg tgttgataga accacttcac ttcaccggta
    27241 gtcgggtcta cgcccacctg gccggaggtg tagaggctgt cgtagtcctt cggatcacca
    27301 ccgtcagcgg tacgcgccca gccattccac ggggccgggt tgccggcacc gatgatgatg
    27361 gtgttggttt ctgcgtcgaa gcttgcgctc tgccacggtg caccgccgcc ctggctccag
    27421 gcggctttct tgccggtcgg gtggttggga tcatccggcc aggacggtgc tttcacgtcg
    27481 ccagtcgggg tgctgtcttt gccgttcagg cggcccatgt ggccttcgac gaaggggcgc
    27541 atccagactt cttcgccggt gtccgggtcg cgggcaaaca gcttgccgac cacgccgaat
    27601 tcgtcaccgg agctgccgtg gatcagcagg gtcttgccgc ttttctggtc tttcaccagg
    27661 gtcggcgcgc cggtcatggt gtagccgacg gcgtggtcgc cgaacttctt gttccacacc
    27721 actttgccgg tgtccttgtt cagcgccacg acgcgggcgt cgagggtgcc gaagtagatc
    27781 ttgtcgccgt agatggcagc gccacggttg accacgtcgc agcacgggcg aatgttgtcc
    27841 ggcaggcggt ggttgtaggt ccacagacgt ttgccggttt ttgcatcgag ggcgaatacc
    27901 cgcgagtagg agccagtcac gtagatcacg ccgtcatgga cgatggcctg ggattcctgg
    27961 ccgcgctgtt tctcgtcacc gaaggagaac gaccaggcgg gggtcagttt gaacacgttc
    28021 tggtcgttga cctgggccag cgggctccag cgctgggcgt tggtgcccat gccgtactgc
    28081 agcacgttgc ttgtggtggt gtggtcgttg gcgatgtcgt cccaggtgac attcttggcc
    28141 agggcgctgc cactcaaggc caggccaccg gccagcagta ggctgtggac ggcaagtgcc
    28201 acggggcgcg gcatgctggg taacgatctt gttgtcatgg ttgtggtttc ccatcgtggt
    28261 ggtgaaggtt gcaggcactg ccttgttggc gacctggcga gccgatagtg aaaccacccc
    28321 gattacgccg aaacggaaaa actcccgctt gcgccgggaa gaatgcccaa accggacatg
    28381 acttagcctt gctgctaaag ccacctgcca cacggccaca agccctacta ccaagggact
    28441 agtcgcgggc caccaaagca gcattcggcg ggcgtggcgc tgttcctaag atgctcgcaa
    28501 gcaagctccg tatgagctgt gtgcgtgcat ttgatgaggc cttgaacatg acaattaaaa
    28561 agaacaccgg ttttgctctt ctgctgttgg ctttctcgac caacctctgg gcgcatggtg
    28621 atgtggtgcc ccaggcggtg aagaccgagg gtctggatcc gctgggcgaa gaatggcttg
    28681 aggagaaccc gtaccggggt aacccgcgcg ccatcgagat cggctcttcg gcctacaacc
    28741 agaactgtgc ggcctgccat ggtctggaag ccaaatccgg cggtatcgcc ccagacctgc
    28801 gcctgctcga agccggcatc accggcgatg agtggttcaa ggagcgggtg atcaacggcg
    28861 ccgtgcgcga tggccgggtg tacatgccga aaatggccga ctacctcagc caggaagcac
    28921 tgtgggccgt gcgcagctac ctggagagtg tggccatcga ggagtaatcg gcatgtctcg
    28981 gcgactggcg tggctggggc tgttgtgtac ggcactgctg gcggtttgta tgccgctgca
    29041 ggcgcaggtg cgcagctatg actcgattgt cgagtccggg gtgctcaagg tggcgctgta
    29101 tcaggatttt gcgccttaca gctttcagca ggacgggcag ccccgaggtg tcgactatga
    29161 cgttgctgcg gctctggctg aggggctggg gctcacactt gaggtgatct gggcgccgcc
    29221 gggcgagaag ctcgacgatg acctgcgtga ttacatctgg cggggccatt acctgcgccc
    29281 acacgaactg gccgatgtga tgctgcgcgt gccctatgac cgcgagttct cctacaagag
    29341 caacgagttc ggcgaactgg tcaacgaact ggtggtgatg tttggccctt accagcgcga
    29401 gcgctggcag gtggcattcg atcgtcgccg gctggacgcg gtgcccagtg tggcggtgtt
    29461 tcagcgccac cccattggcg tcgaggtgga aagcgtgccg tccttttacc tgtcatcagt
    29521 caaagacggc atgctcagcc agcagaccca tcactacggc aatgttccgc aagcgttcgc
    29581 ggcgatgaaa accggtgaag tggatgcggt gatggcgctg cgtggcgagg tcgactggca
    29641 gctcaaactg gcggccgaca gccagctggc gctggccgag aacgcttacc cggacatggg
    29701 caagcaggtc tgggacattg gcatggccgt gcatgaaagc aaccggcagc tggcctatgc
    29761 cctggaagaa aagctcgaag agctgattct cgcgggggcg ctcaaacgca tctacggcca
    29821 ctacggcctg cgttacgagc tgccggggtt gtaccaggag gttgagtagg gcagcaggtc
    29881 aacggcaccg ggaggtgtgg gatggaactg gattggcgtt catttctact gctggcgctg
    29941 tttgccgtgc cctgggacag ctggggcagc cagcgcgacc cggtcacgtc ggtgatgtgg
    30001 gactactacc acgagcgtct gctgggcggc gaagcctttg tcttcgatga gcgggtcaag
    30061 ctcagtgcgc cgccgtttgc cgaggatgcc cgtcaggtgc cgatccaggt cgacgccagt
    30121 gccttcgacg gtcaggtggt gcgcatgctg gcctgggccg agcttaaccc gattgcccag
    30181 atattcgact tccagccggg cgagcaggtg ctgccgcgtc tggctattcg catccgggtg
    30241 gaacaagcca cgccgctgcg ggtggcggtg ctgacccgtg atgggctctg gcatattggc
    30301 tcgacgcaga tcgaggccgc tggtggcggc tgtaccgcgc ccagtgtggt gcgtgcccaa
    30361 cctggctggg aggaacacct gggcgaggtg ctcggcaagc gctttgtcct ggccgatgcc
    30421 aaccgtctgc gcgtgcaggt ggcccacccg atggacaacg gcctggtggg cggcatccca
    30481 gaattctttc tcaatcaggc gcagctgcgt gaccgcagcg gcaacaccct ggccagcctc
    30541 gaactgtttc ccgcagtcag cgaaaacccg accatcagcc tggatgtgaa gggcagcgtc
    30601 gagaccgagc tatggttgcg tgacaacaac ggcaatgaat tcaaagccga actctgaaag
    30661 ccacaaggac ctgccatgat caaagtcagc gtgctgtacc ccaatacccc cggtgtgcgt
    30721 ttcgacatga cctattactg cgagcagcac atggcgatcg ttcgccagtg tctgggcagc
    30781 gcctgccagg gcattgccgt ggatgccggc ttagccagcg ccacaccagg tctgcctgca
    30841 ccctttatcg ccatggggca cctgtacttc gacagcgtgg cggtgttcga gcagagcttt
    30901 gcgccgcatg ccgagcgcat catgggtgac ctgccgaatt tcaccgacag ccagccagtg
    30961 gtgcagatca gcgaggttcg tctcgccccc tgagcggtgg tgctgcccat ttcatcgcga
    31021 ggtgtttatg cgctggatac tgttactggc ggcgctggct agcgcctgcg tgcacgccaa
    31081 cccattcact tacgatctgc agccccggca gattgccgat ggcgtgtggc tgtttgaagg
    31141 cagcacggac aacttctccc aggccaacgg cggcaatatt gtcaacaccg ggtttatcgt
    31201 cacggctgat ggcgtgctgg tgatcgacaa cggcccgtcg cgccggtacg gcgaggccat
    31261 gcgtcaggcg attgcccggg tcactgacaa gccgctgctc aaggtcatgc tgacccatca
    31321 tcaccccgac catgtgctgg gcaatcaggc tttcagtgat gtgccgattg ccgccttgcc
    31381 cggcacccgt gatctgctgg cgaagcaggg cgatagcatg gcggagaaca tgtaccgtct
    31441 ggtgggtgac tggatgcgtg gcactgaagt gctgctgccc aacgaggcgc tgcaagcggg
    31501 ccgccttgaa gtgggtgggc ggccattgca gctgctggcc ctgagcggcc ataccggggc
    31561 ggacctggcg attctcgatg tgcagagcgg tgtgctgttt gccggtgacc tggtgttcta
    31621 ccagcgtgcc ctgaccacgc cgggtacgcc ggggctggac acctggctgg cggatctcga
    31681 ccagttgcag caactgccct ggaagcagat cgtgcccggc catggcccgt tggccagtga
    31741 caatgcgccc tttgtgcaga tgcgcgatta cctcggctgg ctggataagc tgctgcgcgg
    31801 tggtgccgag cagggcgcgg agatgaacga gctgattcgc acgcccattc ccgagcgctt
    31861 tgcccgcgtc agcctgagcc gctacgagct gattcgcagc gttagccatc tttacccgcg
    31921 ttatgagcag gccgcgatgc agcgggtgga tcgttaggtt tttcccgtag ggcgggtgca
    31981 accc
//
LOCUS       DASRUM010000306         2320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2498765, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000306 DASRUM010000000
VERSION     DASRUM010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2498765"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..517)
                     /locus_tag="VFF22_20700"
     CDS             complement(107..517)
                     /locus_tag="VFF22_20700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZX19274.1"
                     /translation="MWNKDKARTDVQRFAGKTSLIAVGAELHGDLRFQGAVQVDGKVI
                     GNLVASEGMVRVSVQGRVEGEIRAPHIVIDGEVVGDVHASGHLELGARARVRGNLYYA
                     LMEMAMGAQIEGRLCRLQDETRPLELPETLDAAQ"
     gene            complement(527..1204)
                     /locus_tag="VFF22_20705"
     CDS             complement(527..1204)
                     /locus_tag="VFF22_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HZX19275.1"
                     /translation="MLGLLLVLCIPLAFYAGGWWQQYSQPAVVAGQPAEPVRDEQLLR
                     EVDELRQRVAVLSSAEKVSQQANEQSRLSIKLLEEQIYKQQQDLAFYKGVLAPASRRE
                     GLRIKTFELQATDRPEHFRYKILLSRVGKGETPLEGQLQVSVVGKQGEQDATLELAAL
                     GADLPDALSEQPIAFAFKHFQAIPEAGRFAELKLPEGFIPREIKVRAEVKGEKPLLRT
                     FKWNKEE"
     gene            complement(1258..2292)
                     /gene="argC"
                     /locus_tag="VFF22_20710"
     CDS             complement(1258..2292)
                     /gene="argC"
                     /locus_tag="VFF22_20710"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HZX19276.1"
                     /translation="MIKVGIVGGTGYTGVELLRLLAQHPQAQVEVITSRSEAGMRVDE
                     MYPNLRGHYDSLAFSVPDVATLGACDVVFFATPHGVAHALAGELLAAGTKVIDLSADF
                     RLQDADEWAKWYGQPHGAPDLLPEAVYGLPEVNREAIKQARLIAVPGCYPTAAQLALI
                     PLLEAGLADTTQVIADCKSGVSGAGRGASVGSLFSEAGESMKAYGVKGHRHLPEIRQG
                     LRRAAGKDVGLTFVPHLTPMIRGIHATLYATVVDRSVDLQALFEQRYGNEPFVDVMPA
                     GSHPETRSVRGANVCRIAVHRPQDGDLVVVLSVIDNLVKGASGQAVQNMNILFGLDER
                     LGLSHAAMLP"
BASE COUNT          445 a          789 c          648 g          438 t
ORIGIN      
        1 agcgctgggg gtgaaggttt cgacgctcat gctgattaac tcccggcgct acgcgccata
       61 ctgcattctg atcggcatta tccgcttacc cgagaaaagc ggtcaactat tgtgcagcat
      121 ccagcgtttc tggtaattcc agtggccgcg tctcgtcttg caagcggcag aggcggcctt
      181 cgatctgcgc gcccatggcc atttccatca gggcgtaata cagattgcca cgcacccgcg
      241 cccgcgcgcc cagctcaaga tggccactgg catgcacatc gccgaccact tcaccatcaa
      301 tcactatatg gggggcgcga atctcacctt caacccttcc ctgcacgctg acgcgcacca
      361 tgccttcgct ggccaccagg ttaccgatga ccttgccatc gacctgcacc gcgccttgaa
      421 agcgcaaatc gccatgcaat tcggcaccga ccgcaatcag gctggtcttg cccgcgaaac
      481 gctgcacgtc agtgcgcgcc ttatctttat tccacataag gaacagtcac tcctctttgt
      541 tccatttgaa tgtgcgcagt aaaggcttct cgccctttac ctcagcacgc accttgatct
      601 cgcgcgggat aaaaccctct ggcaatttca gctcggcaaa gcgtccggcc tcggggatgg
      661 cctggaagtg cttgaaggcg aaagctatcg gctgctcaga cagagcatcg ggcaggtctg
      721 cgcccagtgc cgccagctcg agcgtggcat cctgctcgcc ttgcttgcca acgaccgata
      781 cctgcagttg gccttccagc ggcgtctcgc ctttacccac gcggctaagg agtatcttgt
      841 agcggaaatg ctcaggcctg tccgtggcct gcagctcaaa ggtcttgata cgcaaacctt
      901 cacgacgact ggcgggcgcc aatacgcctt tataaaaagc cagatcctgt tgctgtttgt
      961 agatctgttc ttccagcagc ttgatgctca gacggctctg ctcgttggct tgctggctga
     1021 ccttttccgc actgctcagt accgccacgc gctgacgcaa ctcatcgact tcgcgtagca
     1081 actgctcatc gcgcaccggt tcggccggtt gccccgcaac cactgcaggc tggctgtact
     1141 gctgccacca accgccggca tagaacgcca gcggaatgca cagcaccagc agcagcccga
     1201 gcatgcgccg ccgccgggca cgtcccggat cgcttgggcg aacctcccag cccgccgtca
     1261 aggcagcatg gccgcgtggg acaagcccag gcgctcatcg aggccgaaga ggatgttcat
     1321 gttctgtacc gcctgacccg acgcaccttt gaccaggttg tcgattaccg acagcaccac
     1381 caccagatcg ccgtcctgcg gccgatgcac ggcgatccgg cagacgttgg cgccgcgcac
     1441 gctgcgggtt tccggatggc tgccagcggg catcacatcg acaaaaggct cgttgccata
     1501 gcgctgctcg aacagcgcct gcaggtccac cgagcggtcg accacagtgg cgtaaagcgt
     1561 cgcgtggata ccgcgaatca tcggcgtcag gtgcggcaca aaggtcaggc cgacatcctt
     1621 acccgcagcc cggcgcaggc cctggcgaat ttccggcaga tgccgatggc ctttgacccc
     1681 gtaggctttc atgctctcgc cggcttcact gaacagcgaa cccacactgg caccacggcc
     1741 ggcgccgctg accccggatt tgcagtcggc gatcacttgc gtggtatcag ccagcccggc
     1801 ctccagcagc gggatcagcg ccagctgtgc ggcggtggga tagcaacccg gcacagcaat
     1861 cagacgcgcc tgcttgatcg cttcacggtt gacttccggc aagccataaa ccgcctcagg
     1921 cagcagatcc ggcgcgccgt gcggctggcc gtaccacttg gcccattcgt cggcatcctg
     1981 caggcggaag tcggctgaca ggtcgatcac cttggttccg gcggctagca gctcgccggc
     2041 cagggcgtga gcaacaccat gcggcgtggc gaagaacacc acatcgcaag cgcccagggt
     2101 ggccacgtcc ggcacgctga acgccaggct gtcgtaatga ccgcgcaggt tcgggtacat
     2161 ctcgtcgaca cgcatgccag cctcggagcg cgaggtaatc acctcgacct gcgcttgcgg
     2221 gtgttgcgcc aacaaacgca acagctcgac accggtatac cccgtgccgc cgacgattcc
     2281 gaccttgatc atcacttgcc cctttgcaat accagcagtg
//
LOCUS       DASRUM010000307         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1002649, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000307 DASRUM010000000
VERSION     DASRUM010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002649"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2158
                     /locus_tag="VFF22_20715"
     CDS             <1..2158
                     /locus_tag="VFF22_20715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HZX19277.1"
                     /translation="AKLAFGGDAALAGGGKRPDTSVLIVALQSEPLKLNEQGEGEVSL
                     NIPDFNGELRLMAQAWTDEHYGMGEGKTVVAAPLIAELSAPRFLAGGDETTLALDLSN
                     LSGREQKLDVQLSAQGQLRLAQNPGAAVAPITLADGQRTILRIPVTALGGYGQGRFKV
                     SVNGLALPGEELPPFSREWTLGIRPAYPAQLQHFRAVLKDEPWLLPANALSAFEAAGL
                     EARLAISSRPPLNLGEQIRALKAYPYGCLEQTTSGLYPSLYADAATLKRLGVEAEPDE
                     QRKRSIELGIERLLSMQRYNGSFGLWGADSDEEYWLTAYVSDFLLRAREQGFGVPEEA
                     LKKANERLLRYLQERHLIEVNYSDNANHSRFAVQAYAGYVLARSQQAPLGALRSLYER
                     RSDALSGLPLVHLAVALQKMGDQPRADELLTAGLARGRESNNWLADYGSPLRDQALIL
                     ALLEEHDLAGNRREERLFNLADEVAGQQWLSTQERNSLYLAGRNLLSKPEPSWSAALQ
                     SGSLAFELSNAQPGLKLDGSDLAAPLTIRNSVSKPGDTPLYQQLTLSGYPRQAPAAGG
                     ENLSIYREYLGMDGQALDLNNLQSGELVLVHLAVSAKQRVPDALVVDLLPAGLELENQ
                     NLAQSSASLDDASSAVKEWRESMQSAAIKHQEFRGDRYVAALDVNGYDTTHLLYLARA
                     VTPGSYRVPPPQVESMYRPNWQALGTTPGRLVVKER"
     gene            complement(2210..>2462)
                     /locus_tag="VFF22_20720"
     CDS             complement(2210..>2462)
                     /locus_tag="VFF22_20720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016783546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX19278.1"
                     /translation="GSVIRFIVEGLSGFFGMLGGAVSSFITGMSKALGVTPSLLSIVV
                     LLAGLWLLYLAVRAFIRRSIIAGVIWLVLGLWLLSGLIS"
BASE COUNT          478 a          769 c          819 g          396 t
ORIGIN      
        1 ggccaaactg gccttcggcg gtgacgcggc gctggccggt ggcggcaagc gtccggacac
       61 cagcgtgttg atcgtcgcgt tgcagagtga gccgctgaaa ttgaacgagc agggtgaagg
      121 cgaagtcagc ctgaatatcc ccgacttcaa cggcgaactg cggttgatgg cgcaagcctg
      181 gaccgacgag cactacggca tgggcgaagg caagacggtg gttgctgcgc cgctgattgc
      241 cgagctgtcc gcgccacgct tcctcgctgg cggtgatgaa accaccctgg ccctggacct
      301 gagcaatctg tccgggcgcg agcagaaact cgatgtgcaa ctgagcgccc aaggccagct
      361 acgcctggcg cagaatccgg gggcggcggt ggcgccaatc accctggcgg atgggcagcg
      421 caccattctg cgtattccgg taaccgcact gggcggctac gggcaagggc gcttcaaggt
      481 cagcgtcaat ggcctggccc tgccgggtga ggagctgccg ccgtttagcc gtgagtggac
      541 cctgggcatt cgcccggcct acccggcgca gctgcagcac ttccgcgcgg tgcttaaaga
      601 tgaaccctgg ttgttgccgg ccaatgcgct gagtgcgttt gaggctgcag ggctggaagc
      661 gcggttggcg atttccagcc ggccaccgct taacctcggc gagcagattc gcgcgctcaa
      721 ggcgtatccg tacggatgtc tggaacagac caccagtggc ctgtatccgt cgctgtatgc
      781 cgatgcggcc acgctgaaac gcctgggcgt ggaagccgag ccggatgagc agcgcaaacg
      841 ttccatcgag ctgggcatcg agcgtctgct gagcatgcag cgctacaacg gcagcttcgg
      901 tctgtggggc gcggacagcg atgaggaata ctggctgacc gcctacgtca gcgacttcct
      961 gctgcgcgct cgcgagcaag gcttcggtgt accggaagag gcgctgaaaa aggccaacga
     1021 acgcctgctg cgctacctgc aagagcgtca cctgatcgag gtcaactaca gcgacaacgc
     1081 caaccacagc cgctttgccg tgcaggccta cgccggttac gtgctggcgc gcagccaaca
     1141 ggcgccgctg ggtgccttgc gcagtctgta tgagcggcgc agtgatgcgt tgtccggctt
     1201 gccgctggtg cacctggccg tggccctgca gaaaatgggc gaccagccgc gtgccgatga
     1261 gctgctgacc gccggcctgg cccgtggtcg tgagagcaat aactggctgg ccgactatgg
     1321 cagcccgctg cgcgatcagg cgctgattct ggcgctgctg gaagagcacg acctggccgg
     1381 taatcgccgt gaagagcggc tgttcaacct ggccgatgaa gtggctgggc agcagtggct
     1441 gtcgacccag gaacgcaact cgctgtacct ggccgggcgc aacctgctga gcaaaccgga
     1501 gccgagctgg agcgcggccc tgcaaagcgg cagcctggcc ttcgagctga gcaatgccca
     1561 gccggggctc aagctcgatg gcagtgatct ggctgcgccg ctgactatcc gcaattcagt
     1621 gagcaagccg ggcgatacgc cgctgtatca gcagctgacc ctctccggct acccacgtca
     1681 ggccccagcg gccggtggcg agaacctcag catctaccgt gagtatctag gcatggatgg
     1741 ccaggcgctg gatctgaaca acctgcagag tggcgagctg gtgctggtgc acctggccgt
     1801 atccgccaag cagcgtgtgc cggatgccct ggtggttgac ctgctgccgg cgggcctgga
     1861 gctggaaaac cagaacctgg cgcaaagctc ggccagcctg gatgacgcca gcagtgcagt
     1921 gaaagagtgg cgtgagtcga tgcagagcgc ggcgatcaaa caccaagagt tccgtggtga
     1981 tcgctacgtc gcagcgctgg atgtcaacgg ctacgacacc acccacctgc tgtacctggc
     2041 ccgcgccgtt acccccggca gctaccgcgt gccgccaccg caagtggagt cgatgtaccg
     2101 gccgaactgg caggccctag gcaccacgcc ggggcgtctg gtggtcaagg agcgctagaa
     2161 cgcacaaggc ccgccaaatt ggcgggcctt gttgtttagc cggtgtggct tagctgatca
     2221 gaccgctcag cagccacaat ccgagcacca gccagatcac cccggcaatg atcgagcggc
     2281 ggataaaggc gcgtaccgcc aggtacaaca gccagagacc agccagcaga accacgatgc
     2341 tgagcaatga tggcgtcacc cccaaggcct tggacatgcc cgtgataaag ctgctgaccg
     2401 cgccgccgag catgccgaaa aagccgctca agccttcgac gataaagcgg atcaccgagc
     2461 cg
//
LOCUS       DASRUM010000308         3028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_150612, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000308 DASRUM010000000
VERSION     DASRUM010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150612"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..1366
                     /locus_tag="VFF22_20725"
     CDS             8..1366
                     /locus_tag="VFF22_20725"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HZX19279.1"
                     /translation="MSTKLDQLSSWLKERKITEVECLISDLTGIARGKISPTNKFLDE
                     KGMRLPESVLLQTVTGDYVEDDIYYDLLDEADIDMFCRPDENAVFLVPWAIEPTAMVI
                     HDTFDKQGNPIELSPRNILKNVLKLYADKGWKPIVAPEMEFYLTKRNSDPDFPLVAPM
                     GRSGRPEVGRQSFSIDAANEFDPLFEDVYDWCEIQGLDLDTLIHEDGPAQMEINFRHG
                     EALHLADQITVFKRTMREAALKHDVAATFMAKPITDQPGSAMHIHQSVVDIKTGQNLF
                     SNADGSMSELFMQHIGGLQKYIPELLPLFAPNVNSFRRFLPDTSAPVNVEWGEENRTV
                     GLRVPEATPQNRRVENRLAGADANPYLVLAASLLCGYMGMVDGLQPSVPVKGRGYERR
                     NLRLPITIEHALERMEACKDAEKYLGDKFMRGYVAVKRAEHENYKRVISSWEREFLLL
                     SV"
     gene            1460..2833
                     /locus_tag="VFF22_20730"
     CDS             1460..2833
                     /locus_tag="VFF22_20730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZX19280.1"
                     /translation="MNSQVTNPKTREWQAMSREHHLAPFSDFKQLAENGPRIITKADG
                     VYLWDSEGNKILDAMAGLWCVAIGYGRKELADAAAKQMQELPYYNMFFQTATPPALEL
                     AKAISELAPAGMNHVFFTGSGSEGNDTMLRMVRHYWAIKGQPNKKVIISRVNGYHGST
                     VAGASLGGMKYMHEQGDLPIPGIVHIPQPYWFGEGGDMSPEAFGIWAADQLEQKILEV
                     GEANVAAFIAEPIQGAGGVIIPPDSYWPRIREILAKYDILFIADEVICGFGRTGEWFG
                     SDYFGNTPHMMTIAKGLTSGYIPMGGLIVRDDIVEVLNQGGDFNHGFTYSGHPVAAAV
                     ALENLRILREEKIVERVKAETAPYLQKRLRELADHPLVGEVRGVGMLGAIELVQDKAS
                     RKRFAADVSVGMVCRKHCFTNGLIMRAVGDTMIISPPLVISFAEIDELLDKARKCLDL
                     TAAEVLV"
     gene            complement(2830..>3028)
                     /locus_tag="VFF22_20735"
     CDS             complement(2830..>3028)
                     /locus_tag="VFF22_20735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX19281.1"
                     /translation="DNARRVLPNVFMRTAPVVVVVFRKMRSHLQHLPVSLTYALAQSG
                     KVTPRLTTFRRNHLKPKIIKP"
BASE COUNT          635 a          898 c          914 g          581 t
ORIGIN      
        1 ggtctctatg agcaccaaat tagaccagct ttcgagctgg ctgaaagaac gcaaaatcac
       61 cgaagttgaa tgcctgatca gcgatcttac cggcattgcc cgtggcaaga tttcgccgac
      121 caacaagttc ctcgacgaga aaggcatgcg cctccctgag agtgtgctgc tgcagaccgt
      181 aaccggcgac tacgtcgagg acgacatcta ttacgacctg ctcgacgagg cggacatcga
      241 catgttctgc cgcccggacg agaacgccgt gttcctggtg ccttgggcca ttgagccgac
      301 tgcgatggtg atccacgaca ccttcgacaa gcagggcaac ccgattgagc tgtcgccgcg
      361 caacattctc aagaatgtgc tcaagcttta tgccgacaag ggctggaagc caatcgttgc
      421 gccggaaatg gagttctacc tgaccaagcg caacagcgac ccggatttcc cccttgttgc
      481 gccgatgggc cgctccggtc gcccggaagt cggtcgtcag tcgttctcca tcgatgcggc
      541 caacgaattc gacccgctgt tcgaagatgt ttacgactgg tgtgaaatcc agggtctgga
      601 cctcgatacg ttgatccacg aagacggccc ggcgcagatg gaaatcaact tccggcatgg
      661 cgaggccctg cacctggccg accagatcac cgtgttcaag cgcaccatgc gtgaagcggc
      721 gctcaagcac gacgtggccg ccacctttat ggccaagccg atcaccgatc agccgggcag
      781 cgccatgcac atccaccaga gcgtggtgga catcaagacc gggcagaacc tgttctccaa
      841 tgccgatggc agcatgagcg agctgtttat gcagcacatc ggcggcctgc agaagtacat
      901 ccccgagctg ctgccgctgt tcgcgccgaa cgtcaactcg ttccgccgct tcctgccgga
      961 cacttcggca ccggtgaatg ttgagtgggg cgaagagaac cgcaccgtgg gcctgcgcgt
     1021 accggaagcc acgccgcaga accgccgcgt ggaaaaccgc ctcgccggtg ccgacgccaa
     1081 cccgtatctg gtactcgcag cctcgctgct gtgcggctat atgggcatgg tcgatggcct
     1141 acagccgagc gtgccggtca agggtcgtgg ctatgagcgc cgcaacctgc gcttgccgat
     1201 caccatcgag cacgccctgg agcgcatgga agcctgtaag gatgcggaga agtacctggg
     1261 cgacaagttt atgcgcggct atgtcgcggt gaagcgcgcc gagcacgaga actacaagcg
     1321 tgtcatcagt tcctgggagc gtgagttcct gctcttgtcg gtgtaacagt ccggtggctg
     1381 gcgcaggggc attcctgcgc cagccactca gaatttttat cgggctgcaa cgcgcagcct
     1441 gtgtaagcaa ggtgtcgtta tgaatagtca agtgaccaac ccgaaaaccc gcgagtggca
     1501 ggccatgagc cgcgaacacc acctcgcgcc gttcagcgat ttcaaacagc tggcggaaaa
     1561 tgggccgcgc atcatcacca aggccgacgg cgtgtacctg tgggacagcg agggcaacaa
     1621 gatcctcgat gccatggccg ggctctggtg tgtggcgatt ggttatggtc gcaaggaact
     1681 ggcagacgcc gccgccaagc agatgcaaga gctgccctac tacaacatgt tcttccagac
     1741 cgccacaccg cccgcgctgg agctggccaa ggcgatttct gagctggctc cggccggtat
     1801 gaaccacgtg tttttcaccg gttcgggttc ggaaggtaac gacaccatgc tgcgcatggt
     1861 gcgccattac tgggcgatca agggccagcc caacaagaag gtgatcatca gccgggttaa
     1921 cggctaccac ggctccaccg tggctggcgc cagcttgggc ggcatgaagt acatgcatga
     1981 gcagggcgac ctgccgatcc cgggcatcgt gcacatcccg cagccgtact ggttcggcga
     2041 gggcggcgac atgagccctg aagcgttcgg tatctgggcc gccgaccagc tggagcagaa
     2101 gatcctcgag gttggcgaag ccaatgttgc cgcctttatt gccgagccga tccagggcgc
     2161 aggtggggtg atcatcccgc cggacagcta ctggccgcgt atccgtgaaa tcctcgccaa
     2221 gtacgacatc ctgtttatcg ccgatgaggt gatctgcggt ttcggccgca ccggcgaatg
     2281 gttcggcagc gattatttcg gcaacacccc gcacatgatg accatcgcca aaggcctgac
     2341 cagcggctac atccccatgg gcgggctgat cgtgcgcgac gatatcgtcg aggtgctcaa
     2401 ccagggcggc gacttcaacc acggcttcac ctactccggg cacccggtgg ctgctgctgt
     2461 ggccctggaa aacctgcgca ttctgcgcga agaaaagatt gttgagcgcg tcaaggcaga
     2521 aacggcaccg tatttgcaaa agcgtttgcg tgagttggct gatcacccgc tggtgggtga
     2581 agtgcgcggc gtcggcatgt tgggcgccat tgagctggtg caggacaaag ccagccgcaa
     2641 gcgtttcgcc gccgatgtca gcgttggcat ggtatgccgc aagcactgct ttactaacgg
     2701 tctgatcatg cgcgccgtag gcgacaccat gatcatttcg ccgccgttgg ttatcagctt
     2761 tgctgagatc gacgaattgc tggacaaggc ccgcaaatgc cttgacctga ccgcagcaga
     2821 ggtactggtt tagggtttaa taatctttgg tttcagatgg ttacgtctga atgttgtcaa
     2881 ccggggtgtg accttgccag actgcgccaa tgcgtaggtc aggctcaccg gaaggtgctg
     2941 cagatggctg cgcatcttcc ggaaaacgac tacaacaaca ggagctgtac gcatgaacac
     3001 attcggcaaa acccttctcg cgttgtcc
//
LOCUS       DASRUM010000309         8581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2500349, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000309 DASRUM010000000
VERSION     DASRUM010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8581
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2500349"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..2143)
                     /gene="ileS"
                     /locus_tag="VFF22_20740"
                     /pseudo
     CDS             complement(1..2143)
                     /gene="ileS"
                     /locus_tag="VFF22_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253250.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
     gene            complement(2192..3118)
                     /gene="ribF"
                     /locus_tag="VFF22_20745"
     CDS             complement(2192..3118)
                     /gene="ribF"
                     /locus_tag="VFF22_20745"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HZX19282.1"
                     /translation="MQLVRGLHNLRPQHRGCVATIGNFDGVHRGHQAILARLRERSAE
                     LGVPSCVLIFEPQPREFFAPDTAPARLTRLRDKLALLAAEGVDLVLCLSFNRRLRELS
                     AAEFVQKVLIDGLDIRHLEVGDDFRFGCDRAGDFAFLAEAGEREGFSVEAASTVELNG
                     IRVSSTRVREALAAGDFVLAEHLLGRPFQIAGRVLHGQKLGRQLNAPTANVQLKRKRV
                     PLKGVYLVSTEIDGQIWPGVANIGVRPSVAGDGRAHLEVHLLDFAGDLYGRRLTVAFH
                     HKLRDEQRFASLEALKTAIAADIAAARAHWRV"
     gene            complement(3195..4739)
                     /gene="murJ"
                     /locus_tag="VFF22_20750"
     CDS             complement(3195..4739)
                     /gene="murJ"
                     /locus_tag="VFF22_20750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HZX19283.1"
                     /translation="MNLLKSLAAVSSMTMLSRVLGFVRDTIIARTFGAGVASDAFVVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKTQQGDEAARTFLAYVTGLLTLVLALVTFIG
                     ILAAPWIVWASAPGFADEAERFELTTDLLRVTFPYILLISLSSLAGAVLNTWNRFSVP
                     AFVPTLLNISMIVFALFLTPYFDPPIMALGWAVLVGGLAQLLFQLPHLHKIGMLVLPR
                     LNLKDTGVWRVVKQMGPAIFGVSVSQISLIINTIFASFLVAGSVSWMYYADRLMELPA
                     GVLGVALGTILLPALSKTHAGASREDYSKLLDWGLRLCLVLALPSAVALAIISEPLIA
                     SLFQYGKFTAADTLMTQRALIAYSLGLVGIILVKILAPAFYAQQNIKTPVRIGLVTLL
                     ATQVMNVLFIFVIPLSHAGLALAIGLAACLNAGLLYWQLRKRDMFQPQPGWALFLGKL
                     LLALLAMVAVLLLSMQQLPAWADGTMLWRLLRLGGLVGVGMLAYLAVLALLGFRLRDF
                     ARRSAL"
     gene            4917..5195
                     /gene="rpsT"
                     /locus_tag="VFF22_20755"
     CDS             4917..5195
                     /gene="rpsT"
                     /locus_tag="VFF22_20755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HZX19284.1"
                     /translation="MANTPSAKKRAKQAEKRRSHNASQRSMVRTYIKNVVKAIEAKDV
                     ALATTAYTLAVPVIDRMADKGIIHKNKAARHKSRLNGHIKALGEAAAA"
     gene            complement(5307..5771)
                     /locus_tag="VFF22_20760"
     CDS             complement(5307..5771)
                     /locus_tag="VFF22_20760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009050950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HZX19285.1"
                     /translation="MRVVKGLLGALMLLPGLAAAEQIGEVSTVFKIMGPNDKIVVEAF
                     DDPKVAGVTCYLSRAKTGGVKGGLGLAEDRAEASIACRQVGPISFQGELKEGEEVFRE
                     RTSLVFKTMQVVRFFDEKRNTLVYLVYSDRVIEGSPQNAVTAIPILPWPQKP"
     gene            complement(5782..6900)
                     /gene="proB"
                     /locus_tag="VFF22_20765"
     CDS             complement(5782..6900)
                     /gene="proB"
                     /locus_tag="VFF22_20765"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HZX19286.1"
                     /translation="MRDKVTGAQRWVVKIGSALLTADGRGLDRAAMAVWVKQMVALRE
                     QGVELVLVSSGAVAAGMSKLGWVARPSAMHELQAAAAIGQMVLIQAWESSFAEHGRRT
                     AQILLTHDDLSDRKRYLNARSTLRTLVDLDVVPVINENDTVVTDEIRFGDNDTLAALV
                     ANLVEADLLVILTDRDGMYNADPRHNPDAELIYEARADDPALDAVAGGVGGALGRGGM
                     QTKLRAARLAARSGAHTVIVGGAIEQVLARLKSGERLGTLLAPERGMLAARKQWLAGH
                     LQTRGTLVLDDGAVKALATGTKSLLPVGVKAVQGSFRRGEMVVCVSPDGREIARGLAN
                     YSALEAQKIIGQSSDAIERLLGYVDEPELVHRDNLILI"
     gene            complement(6969..8192)
                     /gene="cgtA"
                     /locus_tag="VFF22_20770"
     CDS             complement(6969..8192)
                     /gene="cgtA"
                     /locus_tag="VFF22_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018925882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HZX19287.1"
                     /translation="MKFVDEVSIFVKAGDGGNGMMAFRREKFIEKGGPNGGDGGDGGS
                     VYIEAQESLNTLVDYRYTRRFQAQNGEKGGSTDCTGAKGEDLILPVPIGTTVIDAGTQ
                     EVIGDLTRAGQRLLVAQGGWHGLGNTRFKSSTNRAPRQTTPGKPGDARDLKLELKVLA
                     DVGLLGLPNAGKSTFIRSVSAAKPKVADYPFTTLVPNLGVVSVDRYKSFVVADIPGLI
                     EGASHGAGLGIRFLKHLSRTRLLLHLVDMAPLDLTDPAESAATIIDELTKFSPSLAER
                     ERWLVLNKADQILDEEQDARIAEIVARIEWEGPVYVISALARQGTEKLCYDIMDFLEA
                     RAERIQENPEYAAELAELDTRIEDEARAQLQALDDKRALRRSGVKAVGDVDEDDSFWD
                     EEDEDDGPEIIYVRD"
     gene            complement(8442..>8581)
                     /locus_tag="VFF22_20775"
     CDS             complement(8442..>8581)
                     /locus_tag="VFF22_20775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HZX19288.1"
                     /translation="RGTQFHAGYGVGMGKDHTLFAKIEGVIKFEVKGAFNRRYVSVVA
                     A"
BASE COUNT         1931 a         2775 c         2374 g         1501 t
ORIGIN      
        1 tactgactcg ttacgctcac ctggcaggta ttcccacagt tcgtcggcgg taaaggcaag
       61 gatcggcgcg atccaacgcc agcatctcgt cgaccggcag cagatgctgc gccgggtcga
      121 agccgctgag gttggagagc aggaagcgcg cggtgttacg aatgcgccga taggagtcgg
      181 cgctgcgctg caggatcacc ttggatacgg ccatctcgcc ggagtagtcg gtggacgaca
      241 cccacaggcg caggatatcg gcgcccaggc tgtcattcac ttcctgcggt gcaaccacgt
      301 tacccaggga cttggacatc ttgcggccgt tctcgtcgac cacaaaaccg tgggtcagca
      361 gctctttgta aggcgcgtgc ccgtcgatgg ccgcgccggt gagcagggag gaatggaacc
      421 agccgcggtg ctggtcggaa ccttccagat acaggtcggc gcgcgggccg ctagcatggc
      481 ccaggttgtg cgagccgcgc agcacgtgcc aatgagtggt accggaatcg aaccaaacgt
      541 ccaaggtgtc gctgatcttg tcgtagtcgg tggcctcatc gccgagcagc tcggctgcat
      601 ccagcttgaa ccaagcctcg atgccctctt gctcgacacg tttggccacc tcttccatca
      661 actcggcgct gcgcgggtgc aactcacccg tggccttgtg caggaagaac gggatcggca
      721 cgccccagtt gcgctgccgc gagatgcacc agtccggacg cccggcgatc atgctgtgca
      781 ggcgcgcctg gccccaggca ggaacgaatg cggtctgctc gattgccgcc aatgcacgct
      841 cgcgaagggt gtcaccttcg ttcggctgct tgtccatgcc gacgaaccac tgcgcggtgg
      901 cgcggtaaat cagcggggtt ttgtggcgcc agcagtgcat atagctgtgc tggatggctt
      961 cgtgcttgag cagcgcgccg acttcttcca gcttggcaac gatggccggg ttggccttcc
     1021 agataaactg gccaccaaag aacggcaggt cgctgacata cacgccgttg ctctgcaccg
     1081 ggctgaggat gtcgtcgttg ctcatgccgt agcctttgca ggagcggaag tcgtcctcgc
     1141 cataggccgg cgccgagtgc accacaccgg taccagcacc taactcgacg tactcggcca
     1201 aatagaccgg tgcgaagcgc tcatagaacg gatggcggaa gcggatcagc tccagcgccg
     1261 cacctgccgc ggtggcgatc acctcgccct gcaggccata acgggccaga caaccttcca
     1321 ccagttcttc ggccagcagc aacaggcgct cgccggtatc gaccagcgcg taggtaaatt
     1381 cagggtggac gttgagcgcc tggttggccg gaatggtcca aggcgtggtg gtccagatca
     1441 cgatggaagc aggcttcggc aggctggcca gaccaaacgc ggcggccagc ttgtcggcat
     1501 cttcaactac gaaggccacg tcgatggcat cggatttctt gtcctgatat tcgacttccg
     1561 cctcagccag ggctgagccg caatcgaaac accagttaac cggcttcaga cccttgaaca
     1621 cgaaaccctg cttgaccatc tcggccaggg cgcggatttc accggcttcg ttggcaaagt
     1681 ccatggtctt gtacggattg tcccagtcgc ccagcacgcc gaggcggata aagtcagcct
     1741 tctgaccttc gatctgctcg gcggcatagg tgcggcagcg ctcgcgggtc aggtcggctg
     1801 gctgattctt gccaaaggtg gtttccacct tgtgttcgat cggcaggcca tggcagtccc
     1861 agcccggcac atagggggcg tcgaagccgg ccagggtctt ggagcgggtg atgatgtcct
     1921 taaggatctt gttgaccgca tgaccgatgt ggatgctgcc gttggcatag ggcgggccgt
     1981 cgtgcagaac gaacttcggc cgaccttcac cttgcgcgcg cagcttctga tacaggccaa
     2041 tctcgttcca gcgctgcaga atctgcggct cgcgctgcgg caggccggcc ttcatcggaa
     2101 attgggtatc aggcagatta agcgtggctt tgtaatcggt catttcaggc tcttcgtaag
     2161 cgattggcac ccagcagttg aagccggggg cttaaacgcg ccaatgggca cgggcggcag
     2221 caatatcggc agcaatagcc gtcttcaatg cctcaaggga ggcgaaacgc tgctcatcgc
     2281 gcagcttgtg gtggaaagcc accgtcaaac gccggccata taaatcaccg gcaaagtcca
     2341 gcaaatgcac ttccagatga gcgcggccat cacctgccac gctcgggcgc acaccaatgt
     2401 tggccacacc cggccagatt tggccgtcaa tctcggtgct gactaggtac acgcccttga
     2461 gcggcacccg cttacgcttg agctgcacat tggcagtcgg cgcattcagc tgacgcccca
     2521 gcttctgccc gtgcaacacc cgcccggcga tctggaacgg gcgcccgagt agatgttcgg
     2581 cgaggacaaa atcgccagca gccagagcct cgcgcacccg cgtactgctg acccggatgc
     2641 cgttcagctc gaccgtgctg gcggcctcga cactgaagcc ctcgcgctcc cccgcctcgg
     2701 ccagaaaagc aaagtcaccg gcacggtcgc agccgaaacg aaagtcatcg ccaacttcca
     2761 gatggcgtat atccaggccg tcgatcagca ctttctgcac gaactcggca gcggacagct
     2821 cgcgcaagcg ccggttgaag ctcaagcaca gcaccaggtc gacgccttcg gcagcgagca
     2881 aggcgagctt gtcgcgcaag cgggtcaagc gcgccggggc ggtatccggg gcaaaaaact
     2941 cgcgcggctg cggctcgaaa atcaacacgc aactgggcac accgagttcg gcggaacgct
     3001 cacgcaaacg cgccaagatg gcctgatgac cacgatggac gccgtcgaaa ttaccgatgg
     3061 tggcgacaca gccccgatgc tggggccgca agttgtgtag gcctcgaacc agctgcataa
     3121 cgcgcttctt gctcataaag tggccgatta tacgcacacc gggcaacgga caacaggcgg
     3181 caaatcagcg ctaatcagag cgcactacgc cgcgcaaagt cccgcaaacg aaaacccagc
     3241 agcgccaaca cggccaaata ggccagcata cccacgccaa ccagcccgcc cagacgcagc
     3301 aaacgccaga gcatagtgcc gtccgcccag gctggcagct gctgcatgct cagcagcagc
     3361 acagccacca ttgccagcag cgcaagcagc aacttaccga gaaacagcgc ccagcccggt
     3421 tgcggttgga acatgtcgcg tttacgtagc tgccagtaca acagcccggc attcagacag
     3481 gcggccagac cgatagccag cgccaagccg gcatgggaca ggggaatcac gaagataaac
     3541 agcacattca tcacctgggt cgccaacagc gtcaccagac cgatacgcac cggggtcttg
     3601 atgttttgct gggcgtagaa ggctggcgcg agaattttca ccagaatgat gccaaccaaa
     3661 cccagtgagt aagcaatcaa ggcgcgctgg gtcatcaagg tatcggcagc ggtgaatttg
     3721 ccgtactgga acagcgaggc gatcagcggc tcagagataa tcgccaacgc caccgcactg
     3781 ggcagcgcca ataccagaca cagacgcaag ccccaatcga gcagcttgga atagtcctcg
     3841 cgactggctc cggcatgagt tttcgacaag gccggcaaca gaatggtccc cagcgccacg
     3901 cccagcacac cggctggcaa ctccatcaga cggtcggcgt aatacatcca ggacaccgag
     3961 ccggcgacca gaaaggaggc gaagatggtg ttgatgatca gcgaaatctg gctgaccgat
     4021 acaccgaaga tcgccggccc catctgctta accacccgcc atacaccggt atccttgagg
     4081 ttcagccgcg gcagcaccag catgccgatc ttgtgcagat gcggcagctg gaacagcagc
     4141 tgcgccaggc cgcccaccag taccgcccag cccagcgcca tgatcggcgg atcgaaatag
     4201 ggcgtcagaa acagggcaaa gacgatcatg ctgatattca gcaacgtcgg cacaaaggcc
     4261 ggtaccgaga agcgattcca ggtgttgagc actgcccccg ccagcgagga cagggagatc
     4321 agcaaaatat aaggaaaggt tacccgcaac agatcggtgg tcagctcaaa acgctccgcc
     4381 tcatcggcaa aaccaggggc agacgcccaa acaatccacg gcgcagcaag aatcccgata
     4441 aaggtcacca gcgccagcac cagggtcagc aggcctgtca cgtatgcgag aaaagtccgc
     4501 gcagcttcat cgccctgctg agtcttgtac tcggccagaa tcggcacaaa ggcctgggaa
     4561 aacgcgccct cggcaaaaat acggcgcagc agattgggca gcttgaacgc caccacaaag
     4621 gcgtccgagg ccacgccagc accgaaagtg cgggcaatga tggtgtcgcg tacaaagccc
     4681 aatacacgcg aaagcatggt catggagctg acagcggcca acgacttgag caaattcatg
     4741 taatatcccg gctccgcaaa caagcactgg cgcgcaaagg cgacgagtgt agcggtctgc
     4801 cactggtcag gccagcacag ggaaaagcca tcaggcttga cagcactccc tcatatcggc
     4861 atgattcgcg gccttatttg tttgtaaccc ccagaatttt ttcgaggagc ttgacggtgg
     4921 ccaatacacc ttctgccaaa aaacgcgcaa aacaggctga gaagcgtcgt agccataacg
     4981 ccagccagcg ctcgatggtt cgtacctaca tcaagaacgt ggtcaaggct atcgaagcca
     5041 aagacgtcgc tcttgctact actgcttaca ccctggctgt gccggtaatc gaccgtatgg
     5101 ccgataaagg catcatccac aagaacaaag ccgctcgtca taagagccgc ctgaatggcc
     5161 atatcaaggc cctgggcgaa gctgcagctg cttaagctgc tagcgacacg ctgattgttc
     5221 ttaaaaaacc ggcttaaacg ccggtttttt attgcccgaa aaatggcaag ccagaaaaaa
     5281 gcccgaatcg cttcgggctt ttttgctcag ggcttttgcg gccagggcag aatcgggatg
     5341 gccgtcaccg cattctgcgg actgccttcg attacccgat cgctataaac cagatagacc
     5401 aaggtattgc gcttttcatc gaagaatcgc accacctgca tggtcttgaa caccagagag
     5461 gtacgctcac ggaagacttc ttcgccctcc ttgagctctc cctgaaagct gatcggccca
     5521 acctggcgac aggcaatcga cgcctcggcg cgatcctcag ccaagcccag accgcccttg
     5581 acgccacccg tcttggcccg cgacaggtaa caggtcaccc cggcgacctt ggggtcatca
     5641 aacgcctcga cgactatctt gtcgttcggc cccatgatct tgaaaaccgt cgacacttct
     5701 ccgatctgct cggccgccgc caacccaggc aacagcataa gcgcacccag caatcccttt
     5761 accactcgca tcagcctccc ctcaaatcag gattaaatta tcccgatgca ccaactcggg
     5821 ctcatcaaca taacccagca gcctctcgat ggcatccgac gactggccga tgattttttg
     5881 cgcctcaagc gcactgtagt tggccaatcc acgagcaatc tcacggccat ccggactaac
     5941 gcaaaccacc atctcaccgc ggcgaaagct accctgcacc gctttcacgc caaccggcag
     6001 caaacttttg gtaccggtcg ccagcgcctt gaccgcacca tcatccagca ccagggtgcc
     6061 acgggtctgc agatgcccag ccagccactg cttacgcgcc gccaacatgc cgcgctcagg
     6121 cgccagcagc gtacctaggc gctcgcccga cttgagccgt gccagcacct gctcgatagc
     6181 accaccgaca atcaccgtat gcgccccgga gcgcgccgcc agacgcgccg cacgcagctt
     6241 ggtctgcatg ccgccacgcc cgagcgcacc accgacacca ccagcgacgg catccagcgc
     6301 cggatcatca gcacgcgcct catagatcag ctcggcatcg gggttatgcc gcggatcggc
     6361 gttatacata ccgtcgcgat ccgtaaggat caccagcaga tccgcctcga ctagattggc
     6421 caccagcgcc gccagagtgt cgttgtcacc gaagcgaatt tcgtcggtga ccacggtgtc
     6481 gttctcgttg atcaccggca ccacatccaa atccaccagt gtgcgcagcg tgctgcgcgc
     6541 attcaggtag cgcttacgat cggacaggtc gtcgtgggtc agcagaatct gcgccgtgcg
     6601 ccggccatgc tcggcaaagc tcgactccca cgcctgaatc agcaccatct gaccgatggc
     6661 tgccgcagcc tgcaactcat gcatggcgct cggccgcgca acccagccca gcttgctcat
     6721 gccagccgcc accgcaccgg aggacaccag caccaactcc acgccctgct cacgcagcgc
     6781 gaccatttgc ttgacccaca ccgccatggc cgcgcgatcc agaccacgcc catcggcggt
     6841 cagcaacgca ctgccaatct tcactaccca gcgctgcgca ccggttacct tgtcacgcat
     6901 gatcttccaa ccttagccgc gacctgtgat tacaaaaacg ccgcaattaa gcggcgtttt
     6961 gtagttgctt aatcccggac gtaaataatt tccgggccat catcttcgtc ttcttcatcc
     7021 caaaagctgt cgtcttcatc gacatcaccg accgccttga ccccggaacg acgcagcgca
     7081 cgcttgtcgt ccaaagcctg cagctgagcg cgcgcttcat cttcgatgcg cgtatccagc
     7141 tcagccagct cggccgcgta ttctgggttc tcctgaatgc gctcagcgcg cgcctcaagg
     7201 aagtccatga tgtcataaca gagcttttcg gtaccctggc gcgccagagc agaaattaca
     7261 taaacaggac cttcccactc gatccgcgcg acgatttcag cgatgcgcgc atcctgctcc
     7321 tcatcgagaa tctggtcagc cttgttcagc accagccagc gctcgcgctc agccagcgac
     7381 ggactgaact tggtcagctc atcaataatg gtggcggccg actcggccgg atcggtcaga
     7441 tccagcgggg ccatatcaac caggtgcagc aacagacggg tacgcgacaa gtgcttgagg
     7501 aagcgaatac ccaagccagc accatgggag gcaccctcga tcagcccagg gatatcggcg
     7561 accacgaagc tcttgtagcg atcgacactg accacaccca ggtttggcac cagggtggta
     7621 aagggatagt cggcgacttt cggcttggcg gccgataccg aacggataaa ggtactcttg
     7681 ccagcattcg gcaaaccgag cagacccaca tcggccaaca ccttcaactc cagcttgagg
     7741 tcgcgcgcat cgcccggctt gcccggcgtg gtctgccgcg gcgcacggtt ggtgctggac
     7801 ttgaagcggg tgttacccag accgtgccag ccaccctgcg ccaccaacaa acgctggccg
     7861 gcacgggtca ggtcgccaat cacttcctgg gtaccggcat cgatcaccgt ggtaccaatc
     7921 ggcaccggca ggatcaggtc ctcgcccttg gcgccggtac agtcggtgct gccgcccttc
     7981 tcaccattct gcgcctggaa acgacgggta tagcggtagt ccaccagggt attgaggctc
     8041 tcctgggcct cgatatacac cgagccaccg tcaccgccat cgccgccgtt ggggccgccc
     8101 ttctcgatga acttttcacg acgaaaagcc atcatgccgt taccgccgtc gccagctttt
     8161 acaaaaatcg atacttcatc gacgaatttc atttttacgc cccccgcaat cacgcgggtc
     8221 atctaaaccg agaaacacca ggttcttgca aaattgacct gcgcccaaac aaacgctaac
     8281 acccaggaca gtttggcaag aaccccaaga atacagaaac agaaaaacgc ctggagcgtt
     8341 ttctaacatc gcctgcgacg actcgcagaa tgactgcaat ggatggcaga ccaataaaaa
     8401 agccccgtca caaggacggg gctttcccag cagtcgcgca attaagccgc aacgacgctc
     8461 acgtaacggc ggttgaacgc gccttttact tcaaacttga tcacgccttc gattttcgcg
     8521 aagagggtgt gatctttacc catgccaacg ccgtaaccgg cgtggaactg ggtgccgcgc
     8581 t
//
LOCUS       DASRUM010000310        12421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1164542, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000310 DASRUM010000000
VERSION     DASRUM010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 12421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12421
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164542"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..2136)
                     /gene="flhA"
                     /locus_tag="VFF22_20780"
     CDS             complement(13..2136)
                     /gene="flhA"
                     /locus_tag="VFF22_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HZX19289.1"
                     /translation="MDRSQMIGDVRSNLAGLKQGNLGIPLLLLVMLGMVMLPIPPFLL
                     DVLFTFSIALSIVVLLVSIYALRPLDFAVFPTILLAATLLRLALNVASTRVVLLNGQD
                     GHDAAGKVIQAFGEVVIGGNYVVGIVVFAILMIINFVVVTKGAGRISEVSARFTLDAM
                     PGKQMAIDADLNAGLIDQSEAKKRRSEVSQEADFYGSMDGASKFVRGDAVAGLLILFI
                     NLIGGIAIGIFQHSLSFVDAGRIYTLLTIGDGLVAQIPSLLLSTAAAIMVTRVSSSED
                     MGAQVNRQMFASPRALAVTAAVMIAMGLVPGMPHFSFISLGLVAAGAAYWIANKQRAT
                     KESEIKEVQRQQDLLPAQKAQEVKELGWDDVTPVDMVGLEVGYRLIPLVDRNQGGQLL
                     ARIKGVRKKLSQEMGFLMPSVHIRDNLDLQPNAYRLTLMGVSVAEAEVYPDRELAINP
                     GQVFGPLNGIAAKDPAFGLEAVWIDPTQRDQAQSLGYTVVDASTVVATHLNQVLHKHA
                     HELLGHEEVQQLMQLLAKSSPKLAEELVPGMISLSTLLKVLQSLLQEQVPVRDIRTIA
                     EAIANVAVKSQDPAAMVSAVRVALARAIVQSIVGLELELPVITLEPRLEQILLNSIQK
                     AGQGSEEGILLEPGMAEKLQRSLVEAAQRQEMLGKPVILLVAGPVRAMLSRFARLAVP
                     SIHVLAYQEIPDNKQVTIVATVGQN"
     gene            complement(2270..3406)
                     /gene="flhB"
                     /locus_tag="VFF22_20785"
     CDS             complement(2270..3406)
                     /gene="flhB"
                     /locus_tag="VFF22_20785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HZX19290.1"
                     /translation="MAESENGADKSEEPTEKRLRESRKKGQIARSKELNTLAVTLSGF
                     GGLLAFGGSMGSAMLDIMRGNFSLSRDVIMDERSMALWLAASGQIALDALFPLFILLL
                     IVSIVGPISLGGWLFSAEAMAPKLSRMDPLAGLKRMFSTNALVELLKALAKFVLILLV
                     GVAVLSAWQDDLLAIVHEPMESAFLHSITVVGWSALWMSCGLILIAAVDVPFQLWSSK
                     QKLMMTKQEVRDEYKDSEGKPEVKQRIRQLQREMAERRMMQAVPDADVVITNPTHFAV
                     ALKYDPAKGSAPVLLAKGGDFTALKIREIANQHKVMVLESPALARAVFYSTELDQEIP
                     AGLYLAIAQVLAYVYQLRQYQAGKGKRPAPLPDMPIPPDLRRDE"
     gene            complement(3410..4186)
                     /gene="fliR"
                     /locus_tag="VFF22_20790"
     CDS             complement(3410..4186)
                     /gene="fliR"
                     /locus_tag="VFF22_20790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HZX19291.1"
                     /translation="MLELSNEQIGAWVGSFLLPLFRISALLMFMPIIGTQLVSARVRL
                     YLSLAICLAIMPTLPPMPVVDAINVQAFIWIAQEVLIGMMLGFVLQLFFQVFVIAGQI
                     VAMQMGLGFASMIDPANGISVPVIGQLFMILVTLLFLAMNAHLVVFEVLTESFFTLPV
                     GGGFLVENYWEIANKLGWVLGAALLLVLPAITALLVINLAFGVMTRAAPQLNIFSIGF
                     PLTLVMGMVIVWIGMTDILAQYQVFATEALQFLREMAGAS"
     gene            complement(4188..4457)
                     /gene="fliQ"
                     /locus_tag="VFF22_20795"
     CDS             complement(4188..4457)
                     /gene="fliQ"
                     /locus_tag="VFF22_20795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HZX19292.1"
                     /translation="MTPEVAVDLVRDALWLTAMIVGILVVPSLIVGLLVAMFQAATQI
                     NEQTLSFLPRLLVILLVLIFAGPWLVRQLMEYTENLIMNIPMLIG"
     gene            complement(4460..5245)
                     /gene="fliP"
                     /locus_tag="VFF22_20800"
     CDS             complement(4460..5245)
                     /gene="fliP"
                     /locus_tag="VFF22_20800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457011.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HZX19293.1"
                     /translation="MLRLFLVLVFSLGASLAFAADAPTASTLIQSGSNPLSIPAITLS
                     TDAAGQQEYSVSLQILLIMTALSFIPAFVMLMTSFTRIIIVFSILRQALGLQQTPSNQ
                     ILVGLALFLTMFIMAPVFDQINREALQPYLNEQMTAQDAIVKAEGPIKNFMLAQTRES
                     DLELFVRLSKRTDIASPEDAPLTILVPAFVTSELKTAFQIGFMIFIPFLIIDMVVASI
                     LMAMGMMMLSPLIISLPFKIMLFVLIDGWALIMGTLAASFGGT"
     gene            complement(5245..5676)
                     /gene="fliO"
                     /locus_tag="VFF22_20805"
     CDS             complement(5245..5676)
                     /gene="fliO"
                     /locus_tag="VFF22_20805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HZX19294.1"
                     /translation="MHRILLLGLALPFAAIAAEPVAQTAVPLAGTGMGGQLVQLLLGL
                     LLVIGLIFLLAWLMRRVQQIVPRGGQVIKILATQALGPRDRLVLVQVGNEQVLLGLTP
                     GTITSLHVLKEPVHLPDAEPASTEFAQRLMELLGKDQKDKS"
     gene            complement(5679..6149)
                     /gene="fliN"
                     /locus_tag="VFF22_20810"
     CDS             complement(5679..6149)
                     /gene="fliN"
                     /locus_tag="VFF22_20810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HZX19295.1"
                     /translation="MADENENEASSPEEQALADEWAAALSEAGDAGQDDIDAMLAASD
                     PAPSAPRAPMEEFDSAPKMNLPVNLDGPNLDVILDIPVSISMEVGNTDITIRNLLQLN
                     QGSVIELDRLAGEPLDVLVNGTLIAHGEVVVVNEKFGIRLTDVISPSERIKKLR"
     gene            complement(6182..7153)
                     /gene="fliM"
                     /locus_tag="VFF22_20815"
     CDS             complement(6182..7153)
                     /gene="fliM"
                     /locus_tag="VFF22_20815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HZX19296.1"
                     /translation="MAVQDLLSQDEIDALLHGVDDGLVETEDEAEPGSVKQYDLTSQD
                     RIVRGRMPTLEMINERFARYTRISMFNLLRRSADVAVGGVQVMKFGEYVHSLYVPTSL
                     NLVKMKPLRGTGLFILDAKLVFKLVDNFFGGDGRHAKIEGREFTPTELRVVRMVLDQA
                     FIDLKEAWHAVMDINFEYVNSEVNPALANIVSPSEVIVVSTFHIELDGGGGDLHITMP
                     YSMIEPIREMLDAGFQSDVDDQDERWIKALREDILGVSVPLGATVARRQLKLRDILHM
                     QPGDVIPVELPDSVVLCANGVPSFKVKMGAHKGKLALQVLGPIERNR"
     gene            complement(7167..7694)
                     /gene="fliL"
                     /locus_tag="VFF22_20820"
     CDS             complement(7167..7694)
                     /gene="fliL"
                     /locus_tag="VFF22_20820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HZX19297.1"
                     /translation="MAKKEAAPAAANAEAKPAGKLKLIIIIVIAVLLAVGLSVGGTWF
                     FLSKQDAGADEAKAEASAEPAVPVKQAAIYEELSPAFVVNFNHQGRARYMQVSVALMT
                     RDQAALDALKVHMPVLRNRLVMLFSGQDFASLITPVGKEMLRQQATASVQELAEKETG
                     KVTVEQVLFTNLVLQ"
     gene            complement(7927..9192)
                     /locus_tag="VFF22_20825"
     CDS             complement(7927..9192)
                     /locus_tag="VFF22_20825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019396587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HZX19298.1"
                     /translation="MSVAPDLLLKSAPEVKPRAAASKPPEKPAQPNRNEASSFSQVYA
                     RERQAKAAERSDAPAKPARESKVDKEEPQDPQAQAAAGQAEVADSGKPLPTDPASEDA
                     VLDPLLLLGITGEMPAEEAEELALDAEPAPLLVPGSSLTSSGPASMTEASFDAELDAL
                     NQLPTVRMALEMGAKEKALAEQAAAPLAQANLATQSASQSMLASQVVQDEMPVEGDAP
                     ELPELQLEALTGKGLEALKEGTANNTPENFVSKLNALSQAIGQQNPLSARAPVVVGQP
                     VPMQQGGWSEAVVDRVMVMSSQNLKSAEIQLDPAELGRLEVRISVNQEQSQVTFASPH
                     AGVREALDSQMHRLRELFAQQGMNQLDVNVSDQSLSRGWQGQEGDGGKGRGGSSDELL
                     GADEGLHSSTVESARATSVSSRGLVDYYA"
     gene            complement(9309..9662)
                     /locus_tag="VFF22_20830"
     CDS             complement(9309..9662)
                     /locus_tag="VFF22_20830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HZX19299.1"
                     /translation="MSDIHLDSAVLAALQDVMEDEYPVLLDTFLIDSEERLLVLRQAE
                     RAADAQSLRLAAHSFKGSCSNMGALMLAGLCKQLEEAGRREQLEQAPELVEQIEREFA
                     IVRILLKSERQRYRH"
     gene            complement(9694..11388)
                     /locus_tag="VFF22_20835"
     CDS             complement(9694..11388)
                     /locus_tag="VFF22_20835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HZX19300.1"
                     /translation="MPERLSILIAEDNAADRMLLSTIVSRQGHRVLTASNGLEAVALF
                     EQERPQLVLMDALMPVMDGFEAARRIKQMAGEELVPIIFLTSLTEGEALVRCLEAGGD
                     DFLAKPYNRVILEAKIKAMDRLRRLQDTVLQQRDLIAIHNEHLLTEQRVAKAVFDKVT
                     HSGCLDAPNIRYLQSPYAVFNGDLLLAAFKPSGGMHVLLGDFTGHGLPAAIGAMPLAE
                     VFYGMTAKGYSLAEVLREINAKLKRILPVGVFCCATVLNISFQRQVVEVWNGGLPDGY
                     LLRGSGERLPLVSRHLPLGVLEPSAFNDKYEVYPLALGDRVFLLSDGVLETCNKQGEL
                     FGEGRLLEVFAANRQPATLFSEIQQSLTHFSGEQQDDVSLLEVSLVEDGDLSRPPLAF
                     ADNGQSHSLDWSASFEFRGETLRHFNPLPFLLQLLLEVQGLRPQGGALFSVLAELYSN
                     ALEHGVLGLDSALKANAEGFAEYYRQRSVRLAALTDGFVRFHLQLLPEGAGGRLIVRV
                     EDSGSGFDAQQLPTEPRDAHSLSGRGLALVRQLSERCSWGDGEPGVRVEFSWLPGA"
     gene            complement(11403..11708)
                     /locus_tag="VFF22_20840"
     CDS             complement(11403..11708)
                     /locus_tag="VFF22_20840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZX19301.1"
                     /translation="MAITSLPSSDGQELTILIQGRFDFGAHQEFRNAYERVNTTPQRY
                     VVDLKDTTYLDSSALGMLLLLRDHAGGDSAQIRLLNCNPDVRKILAISNFEQLFQIA"
     gene            complement(11852..12304)
                     /gene="fliJ"
                     /locus_tag="VFF22_20845"
     CDS             complement(11852..12304)
                     /gene="fliJ"
                     /locus_tag="VFF22_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HZX19302.1"
                     /translation="MAQSRAARLAPVVDMAARAEREAALQLGHAQGLLRQAEVQLGDL
                     ERYRGDYQQQWISEGQRGVSGQWLMNYQRFLTQLETAIGQQRNSVDWHRANMEKVREV
                     WQQRYARLEGLRKLVKRYQDEARMAEDKREQKLLDELSQRISARDAQS"
BASE COUNT         2714 a         3904 c         3423 g         2380 t
ORIGIN      
        1 ctgtaacctc ggttagttct gccccaccgt cgcaacgatg gtgacctgct tgttatccgg
       61 aatttcctgg tatgccagta catggatact cggaaccgcc agccgcgcga atcgcgagag
      121 catcgcccgg accggaccgg caaccagcag tatcactggc ttgccgagca tctcctggcg
      181 ctgcgccgct tccaccaggg aacgctgcag cttctcggcc atgccaggct ccaggaggat
      241 gccctcctcg gagccttgac cggccttctg aatactgttg agcaatatct gttccaacct
      301 gggctccaag gtgatcacag gcagctccag ctctagtccc acaatgcttt gcacaattgc
      361 acgggccaac gcgacacgta cggccgacac catcgcggcg ggatcttgac tcttcactgc
      421 gacattggcg atagcttcgg cgatggtgcg gatatcgcgc accggcacct gttcctgcag
      481 caggctctgc agcaccttga gcagggtcga cagggaaatc attcctggca ccagctcttc
      541 agccagcttg ggcgagctct tggccagcag ctgcatcaac tgctgcactt cctcatggcc
      601 gagcagctcg tgagcatgct tgtgcagcac ttggttgagg tgagtagcga ctacagtgct
      661 ggcgtccacc acggtatagc cgagggactg cgcctgatcg cgctgagtcg ggtcgatcca
      721 caccgcttcc agaccgaatg ctgggtcttt ggcagcgatg ccgttgagcg ggccgaacac
      781 ctggcctggg ttgatcgcca gttcgcgatc cggatacacc tcggcctcgg ccacgctgac
      841 acccatcagg gtcaggcgat aagcattggg ctgcaaatcg aggttgtcgc ggatatgcac
      901 cgagggcata agaaagccca tctcctgaga gagtttcttg cgcacgcctt tgatccgcgc
      961 cagcagctgg ccaccttggt tgcggtcaac caagggaatc aggcggtagc ccacttccag
     1021 gccgaccata tccactggcg ttacgtcgtc ccaacccagc tccttgacct cctgggcctt
     1081 ctgcgccggc agcaagtctt gttggcgctg tacttccttg atctcgcttt ccttggttgc
     1141 acgctgcttg ttggcaatcc agtacgcggc acccgcagcc accaggccca gactgataaa
     1201 ggagaaatgc ggcatgccgg gcaccaggcc catggcgatc atcaccgcag ccgtcaccgc
     1261 caatgcgcgg ggcgaggcga acatctgccg attaacctgc gcgcccatgt cttcagagct
     1321 ggaaacccgg gtgaccatga tcgccgcggc agtcgacagc agcagcgatg gaatctgcgc
     1381 caccagaccg tcaccgatag tcagcaaggt gtagatgcgc ccagcgtcaa caaaactcag
     1441 agagtgctgg aatatgccga tggcaatgcc accgatcagg ttaataaaca gaatcagcaa
     1501 gccggcaacc gcatcacccc tgacgaattt gctggcaccg tccatggatc cgtagaagtc
     1561 ggcctcctgg gacacttcac tgcggcgctt cttggcttcg ctctgatcaa tcaggccggc
     1621 gttcaggtcg gcgtcgatgg ccatctgctt accgggcatg gcatccaggg tgaaacgtgc
     1681 gctgacttcg gatatacgcc cggcgccctt ggtgaccacc acgaagttga tgatcatgag
     1741 gatggcgaac accacgatac cgaccacgta gttgccgccg atcaccactt caccgaaggc
     1801 ctggatcacc ttaccggcag cgtcgtgacc atcttggccg ttgagcagca ccactcgtgt
     1861 ggaggcgacg ttgagcgcca ggcgcagcaa ggttgccgcc agcagaatgg tcgggaatac
     1921 cgcgaaatcc agtgggcgca acgcatagat gctgaccaac aacaccacga tcgacaaggc
     1981 aatactgaag gtgaacaaca catcgagaag aaacggagga atcggcaaca tgaccatgcc
     2041 gagcatgacc agcagcaata gcggaatacc cagattgccc tgcttcaacc ctgccaggtt
     2101 gctgcgcaca tcaccgatca tttgcgagcg atctattgcc acaccactac cccagcccat
     2161 tcaatctttt gacgcgagtc gcgcctctgg ggagggtttt gcaagaagct gtccaacttt
     2221 gccaggccgc gcaaaacagc agccaagcca gctgcaactc ggcctactgt cactcgtcgc
     2281 ggcgtaaatc cggcggaatt ggcatatccg gcagcggcgc cgggcgcttg cccttgcccg
     2341 cctggtactg gcgcaactgg tagacgtagg ccagcacctg ggcgatggcc aggtacaggc
     2401 cggcgggaat ttcctgatcc agctcggtgg aatagaacac cgcccgcgcc agcgctggcg
     2461 actccagcac catgaccttg tgctgattgg cgatttcacg aatcttcaat gcagtgaagt
     2521 caccaccctt ggccagcagc accggtgcac tgcccttggc cgggtcgtac ttcaaggcca
     2581 cggcaaagtg cgtcgggttg gtgatgacca cgtcggcgtc cggtaccgcc tgcatcatgc
     2641 gccgctctgc catttcgcgt tgcaactggc gaatgcgctg cttgacctcg ggcttgcctt
     2701 cgctgtcctt gtactcgtcg cgcacttcct gcttggtcat catcagcttc tgcttgctgc
     2761 tccagagctg aaatggcaca tccacggcgg caatcagaat caacccgcag gacatccaca
     2821 gcgcgctcca acccaccacg gtaatcgagt gcaaaaacgc cgactccatg ggctcatgca
     2881 caatcgccaa caggtcgtcc tgccaggccg acagcaccgc gacgcccacc agcagaatga
     2941 gaacgaactt ggccaaggcc ttaagcagct caaccagtgc attggtcgag aacatgcgct
     3001 taagccctgc taaggggtcc attcggctga gcttgggcgc catggcttcg gcagaaaaca
     3061 gccagccgcc gagagaaatc ggcccgacga tggacacgat cagcagcaag ataaacagcg
     3121 gaaacaacgc gtcaagcgcg atttgccctg aagccgccag ccacaaggcc atgctgcgct
     3181 catccataat cacatcacgg gatagcgaaa agttaccacg catgatgtca agcatggcgc
     3241 tgcccatgct gccgccgaag gccaacaaac caccaaaccc gctgagggtt accgccaggg
     3301 tattgagctc tttagaacgg gcaatctggc cctttttacg ggattcgcgt aagcgtttct
     3361 ccgtgggttc ctcgctttta tcggcaccat tctcggattc ggccatggct taactcgccc
     3421 ccgccatttc acgcaggaat tgcaaagcct cagtggcaaa tacctggtat tgcgcaagga
     3481 tatcggtcat gccgatccag acgatgacca tacccatcac caacgtcagc ggaaaaccga
     3541 tggagaagat attcaactgc ggcgcggcgc gggtcatcac cccgaatgcc aggttgatca
     3601 ccaacagtgc ggtaatcgcc ggcaatacca aaagcagcgc ggcacccagc acccagccca
     3661 gtttgttggc aatttcccag taattctcta ccagaaagcc accgccaacc ggcagggtga
     3721 aaaagctctc ggtcagcact tcgaacacca ccagatgggc gttcatggcg agaaacagca
     3781 aggtcaccag aatcatgaac aactgaccga tcaccggtac agaaataccg ttagccgggt
     3841 caatcatgga ggcaaagccc agacccattt gcatggcgac gatctgcccg gcaatcacga
     3901 aaacctggaa gaacagctgc agaacaaagc ccagcatcat gccaatcaga acctcctgag
     3961 cgatccagat aaacgcctgt acgttgatgg catcgaccac tggcatcggt ggcagagtcg
     4021 gcatgatcgc caggcagatg gccagggata gatataggcg tacccgggcc gacaccagct
     4081 gagtaccgat gatcggcata aacatcaaca gcgccgaaat gcgaaacagc ggcagcagaa
     4141 aactgcccac ccaagcgccg atctgctcgt tgcttagctc aagcatgtca gccgatcagc
     4201 atcggaatat tcatgatcag gttctcggtg tactccatca gctgtcgcac cagccaggga
     4261 ccggcaaaaa tcagcaccag caaaatgacc aacaacctgg gtaaaaaact caaggtctgc
     4321 tcgttgattt gagtggccgc ctgaaacatc gccaccaaca aaccaacaat caggctgggc
     4381 accactagaa ttcccacaat catggccgtc agccacaagg catcgcgcac cagatcgaca
     4441 gccacctcgg gggtcatggt taggtgcctc cgaagctggc ggcgagggtg cccatgatca
     4501 gcgcccagcc atcgatcaac acaaacagca tgatcttgaa cggcagggaa atgatcagcg
     4561 gcgagagcat catcatcccc atcgccatca gaatactggc gaccaccatg tcgatgatca
     4621 gaaacgggat aaagatcata aagccaatct ggaaggcagt tttcagctcg gaggtgacaa
     4681 acgcaggcac caagatggtc agcggtgcat cttccgggct ggcgatatca gtccgtttgg
     4741 acaagcgcac aaacaactcc agatcgcttt cgcgggtctg cgccagcata aaattcttga
     4801 tcggcccttc ggccttgacg atggcgtcct gcgccgtcat ctgctcgttc aggtagggct
     4861 gcaaggcctc gcggttaatc tgatcaaaca ccggcgccat gataaacatg gtcagaaaca
     4921 gcgccagacc gaccagaatc tggttcgacg gcgtctgctg gagccccagg gcctgacgca
     4981 gaatcgagaa gacgatgatg atccgggtga agctggtcat cagcatgaca aacgctggaa
     5041 taaaactcag cgcagtcatg atcagcagga tctgcaggct gaccgaatac tcctgctgcc
     5101 cagcagcgtc ggtgctcagg gtgattgccg ggatcgacag cggattgctg cccgattgaa
     5161 tcaaggtgct ggccgtcggc gcatctgcag cgaacgccag cgaagcaccc aggctaaaaa
     5221 ccaacaccag aaacaaacgc agcatcagga cttgtccttc tggtccttgc ccaacagctc
     5281 catgaggcgc tgggcaaact cggttgaggc gggttcggca tctggcaaat gcaccggctc
     5341 tttcaataca tgcagcgaag tgatggtgcc tggcgtcaac cccagcaaca cctgctcgtt
     5401 gcccacctgc accagcacca gacgatcacg cggccctaag gcctgggttg ccaggatttt
     5461 gatcacctgg ccgccacgcg gcacgatctg ctgcacccga cgcatcagcc aggccagcag
     5521 aaagatcaaa ccgatcacca gcagcaagcc cagcaacaac tgtacaagct gcccacccat
     5581 gcccgtcccg gcaagaggca cagccgtttg cgctaccggc tccgccgcta tcgcagcgaa
     5641 aggcagtgcc aggcccaata gaaggattct atgcatggtt agcgcagctt cttgatacgt
     5701 tcgctcgggc tgatcacgtc ggtcaggcga atgccgaact tctcgttcac taccaccact
     5761 tcaccgtggg caatcagggt accgttgacc agtacatcca gcggctcgcc ggctaggcga
     5821 tccagctcaa tcaccgatcc ctggttaagc tgcagcaggt tgcggatggt gatatcggta
     5881 ttgccgactt ccatggaaat cgacaccggg atatctagga tcacatccaa gttcggcccg
     5941 tccagattaa ccggcaggtt catcttcggc gcgctgtcga actcttccat cggcgcacgc
     6001 ggcgcagatg gcgccggatc gctggcggcg agcatagcgt cgatatcgtc ctgcccggca
     6061 tcgccggcct cggacaatgc agccgcccac tcatcggcaa gtgcctgctc ttcgggggag
     6121 ctggcttcat tttcgttttc gtctgccatc gtttttcctc ggctggcgtt gaatagggag
     6181 attagcggtt acgctcaatg gggccgagca cctgcaaagc cagtttgcct ttgtgcgccc
     6241 ccatcttgac cttgaatgaa ggcacgccgt tggcgcagag caccacgctg tccggcagtt
     6301 ccaccggtat cacgtcaccg ggctgcatat gcagaatgtc gcgcagcttg agctggcgac
     6361 gggcaacagt cgcacccagg ggcacgctga caccaagaat gtcttcgcga agcgccttga
     6421 tccagcgttc gtcctgatcg tcgacatcgg actggaaacc ggcgtcgagc atttcgcgga
     6481 tcggctcgat catcgaatag ggcatggtga tatgcagatc gccaccaccg ccatccagtt
     6541 cgatatggaa ggtggacacc acgatcactt cactggggct gacgatgttg gccagcgccg
     6601 ggttcacttc cgagttgacg tactcgaagt tgatatccat taccgcgtgc caggcttcct
     6661 tgaggtcgat aaacgcctga tccagcacca tgcgcaccac gcgcaactcg gtgggcgtaa
     6721 actcgcgccc ctcgattttg gcgtgacgac cgtcgccgcc aaaaaagttg tccaccagtt
     6781 tgaacaccag tttggcgtcg agaataaaca ggccggtacc gcgcagcggc ttcatcttca
     6841 ccaggttgag gctggtaggc acatacagcg agtgtacgta ctcgccgaat ttcatcacct
     6901 gcacaccgcc caccgccacg tcggcggaac gacgcagcag attgaacata ctgatgcggg
     6961 tgtagcgggc aaaacgctcg ttgatcatct caagcgttgg catgcgccca cggacaatcc
     7021 ggtcctggct ggtcaggtca tattgtttga cgcttcccgg ttcggcttcg tcctcggttt
     7081 caaccaagcc gtcatcaacg ccatgcaaca gggcatcgat ttcatcctgg gaaagcaggt
     7141 cttgcacagc catatacggc tcctgattac tgcaacacga ggttggtgaa caacacttgc
     7201 tcgaccgtaa ccttgccggt ctctttctcg gccagttcct gcacactggc ggtcgcctgc
     7261 tggcgcagca tttccttgcc caccggggta atcagggaag caaagtcctg accggaaaac
     7321 agcatcacca ggcgattacg caacaccggc atatgcactt taagcgcgtc caaggcggcc
     7381 tgatcgcgag tcatcagggc cacgctgacc tgcatatagc gggcacggcc ttgatgattg
     7441 aaattcacca cgaaggccgg cgatagctct tcatagatcg ccgcctgttt gactggcacc
     7501 gcgggctcgg cactggcttc tgccttggcc tcatcagcac ccgcgtcctg cttgctcaga
     7561 aaaaaccagg tgccccccac ggaaaggccc acagccagca acacagcaat aacgatgata
     7621 atgatcagct tcaatttgcc ggctggtttc gcctcggcat ttgccgctgc gggtgctgct
     7681 tctttcttag ccatgccaat aatccgtcgc tattcggagt tctatccgtt ttcaaagggt
     7741 taagagcaag tgttatgcca gtgtgtcaac aaacctgctc actacggcgg cccagcacct
     7801 gagaggtgtg tggccaaggc atcagctata cgcacgcccc aaacaaaatc gcccggctcc
     7861 acgcaatggg agccgggcga aatgcagttt agatcagtgg ggcatgcaac tacagcagca
     7921 agcacgctat gcgtagtaat caaccaagcc acgggagctc acagacgtcg cgcgcgcaga
     7981 ttcgacagta ctcgaatgca gcccctcatc cgcgccgagc agctcatcgg aggagccacc
     8041 gcgacctttg ccgccatcgc cctcctgccc ttgccagccg cggctaagcg actgatcgga
     8101 gacgttcacg tccagctggt tcatgccctg ctgggcgaac agctcacgca ggcggtgcat
     8161 ctgcgaatcc agcgcctcac gcaccccggc atgcgggctg gcgaaggtaa cctggctctg
     8221 ctcctggttg acgctgatac gcacttccag acggcccagc tcggctggat caagctgaat
     8281 ttcggcggac ttgaggttct ggctggacat caccatcacc cgatccacca ccgcttcact
     8341 ccaaccgccc tgctgcatag gcaccggctg cccaaccacc accggcgcgc gggcggacag
     8401 tgggttctgc tgaccaatgg cttggctcag ggcattgagc ttgctgacga agttctccgg
     8461 cgtgttgttt gccgtacctt ccttcagcgc ttcaagacct ttgccggtaa gggcttcgag
     8521 ctgtaactcc ggcagctccg gggcatcacc ctctaccggc atctcatcct gcaccacctg
     8581 gctggccagc atgctctggc tagcgctctg cgtagccaga ttggcctggg ccaaaggcgc
     8641 agcagcctgt tcggccaggg ctttttcctt cgcacccatt tccaatgcca tgcgcaccgt
     8701 cggcagttgg ttaagcgcat ccagttcggc atcgaaactg gcttcggtca tgctcgcggg
     8761 gcctgaacta gtcaggctgc tgcctggcac cagcagtggc gcaggctcag catcaagcgc
     8821 caactcttca gcctcttccg ctggcatctc gccggttatc ccgagcaaca gcagcggatc
     8881 aagcaccgca tcttcgcttg ccggatcagt cggcaaaggc ttgccgctat cggcaacctc
     8941 ggcctgccca gcggcagcct gcgcctgcgg atcttgcggc tcttctttgt ctaccttgct
     9001 ctcacgcgca ggcttggcag gcgcatcact gcgctctgcc gccttggcct gacgctcccg
     9061 ggcatacacc tgagagaagc tggaagcctc atttctattg ggctgcgcgg gtttttccgg
     9121 cggttttgat gcagcagccc tgggctttac ttcaggtgcc gacttgagca gcagatcggg
     9181 ggcaacggac atggatactc tccgttcggg gttggtcgtg cggagagttt tgcaaggcgc
     9241 gggccaactt ggtggcaggc aggttcagcg gggtgaaata cagctataca gggacaaaaa
     9301 tagcaagact agtgccgata acgctgacgc tcggacttaa gcaggatgcg cacaatggca
     9361 aactcacgct caatctgctc aaccaactct ggagcctgtt ccagctgctc gcgccgtccg
     9421 gcctcctcca gctgcttgca cagcccagcc agcatcagcg cgcccatatt gctgcaactg
     9481 cctttgaaac tgtgcgcggc caaccgcagg ctctgcgcat cggcagcccg ctcagcctgg
     9541 cgtaacacca gcaggcgctc ttcggagtcg atcaggaaag tatccagcag caccgggtat
     9601 tcatcttcca tgacgtcctg caaagccgcc aatactgcgc tatcaaggtg aatatcggac
     9661 aactgctcgc tccccctgga gttatgcccc ggattatgcc ccaggcaacc aggaaaactc
     9721 cacgcgcacc cccggttcgc cgtcgcccca actgcaacgc tcgctcaatt gcctgaccaa
     9781 cgccagcccg cgaccgctca agctatgggc atcgcggggc tctgtcggca attgctgggc
     9841 atcgaaaccc gagccgctgt cttcgacccg gacgatcaac cgcccacccg caccctcagg
     9901 cagtaattgc aggtgaaagc gcacaaaacc gtcggtcaac gcggccagcc gcacgctgcg
     9961 ctggcggtag tactcggcaa aaccctcggc attcgccttg agcgccgagt ccaggcccag
    10021 cacaccgtgc tccagagcat tcgaatacag ctcggcgagc acactgaaca aggcgccgcc
    10081 ctgaggccga aggccctgca cctcaagcag cagctgcaag agaaacggca acggattgaa
    10141 atggcgaagg gtttccccgc gaaactcgaa gctggccgac cagtccagtg aatggctctg
    10201 gccattgtcg gcaaaggcca gcggcggtcg gctcagatca ccatcttcga ccaggctgac
    10261 ctcaagcagg ctgacatcat cctgctgctc gccactgaaa tgggtcagcg actgctggat
    10321 ttcagagaac aacgtcgctg gctgccgatt ggcagcgaag acctcaagca agcgcccttc
    10381 accaaacaac tcgccctgtt tgttacaagt ctccagaaca ccatcggaga gcaggaaaac
    10441 ccgatccccc agcgccagcg gatagacctc atacttgtcg ttgaatgcgc ttggctccag
    10501 cacccctaat ggcaaatgcc gcgacaccag cggcaaacgt tcgccactgc cgcgcagcag
    10561 atagccatcc ggcaagccgc cgttccacac ctcgaccacc tggcgctgga aactgatatt
    10621 cagcaccgtg gcgcagcaga acaccccaac cgggagaatg cgtttgagct tggcgttgat
    10681 ctcacgcaga acctcggcca gggagtagcc cttggccgtc atgccataaa acacctcggc
    10741 cagtggcatc gcaccaatcg ctgcaggcaa accgtggccg gtgaagtcgc ccagcagcac
    10801 atgcatacca ccagacggtt tgaacgcggc cagcagcagg tcaccgttaa acaccgcata
    10861 gggcgattgc agatagcgga tattcggcgc atccaggcag ccggaatggg tgaccttgtc
    10921 gaacaccgct ttggccaccc gctgctcggt gagcaagtgt tcgttgtgta tggcaatcag
    10981 atcgcgctgc tgcagcacgg tgtcctgcaa gcgccgcagg cgatccatgg ctttgatctt
    11041 ggcttcgaga atcacccggt tataaggctt ggctagaaaa tcgtcaccgc cggcttccag
    11101 gcagcgcacc agggcttcgc cttcggtcag tgaggtaaga aagatgatcg gcaccagctc
    11161 ttcgccagcc atctgcttga tccgccgggc ggcttcaaaa ccgtccatga cgggcatcaa
    11221 ggcgtccatc agcaccaact gcggccgctc ctgctcgaac agggccaccg cctccaggcc
    11281 attgctggcg gtcagtaccc ggtgcccctg gcgactgacg atggtcgaca gcagcatgcg
    11341 atcagccgcg ttgtcctcgg caatcagaat ggacagacgc tctggcatag cgtgcgcccc
    11401 gtttaagcga tctggaacag ctgctcgaaa ttcgagatag ccaggatctt gcgtacatcc
    11461 gggttacagt tgagcaggcg gatctgcgca ctgtcgccgc cagcgtgatc acgcaacagc
    11521 aacagcatgc ccagcgccga gctgtccagg tatgtggtgt ccttaaggtc tacaacgtag
    11581 cgctgcgggg tggtgttgac gcgctcgtag gcgttgcgaa attcctgatg cgcaccgaaa
    11641 tcgaagcgtc cctgaatcag aatggtcagc tcttgcccat cggatgaggg cagcgaggtg
    11701 atggccatga attactccta taaacgaaaa gaccaggcac acgttcaggt gggccagacg
    11761 caggttatgc atgatcaaga tgtagcatcg caatgcccat gcggcaactg ttaggactgt
    11821 gttgatgtgt ctcggttcag ctatttagcg cttaagactg tgcgtcacgg gcggatatac
    11881 gctgcgagag ttcatcaagc aatttctgtt cgcgcttgtc ttcggccata cgcgcttcgt
    11941 cctgatagcg tttgaccagc ttgcgcaaac cttccaggcg cgcgtagcgc tgctgccaga
    12001 cctcgcgcac cttttccata ttggcgcggt gccagtcgac gctgttgcgc tgctggccga
    12061 tggcagtttc cagttgggtg agaaagcgct ggtagttcat cagccattgc ccggaaacgc
    12121 cgcgctggcc ttcgctgatc cattgctgct gataatcccc gcggtagcgc tccagatccc
    12181 ccagctgtac ttcggcctga cgcagcaaac cctgagcatg gcccaactgc aaggcggcct
    12241 cgcgctcggc gcgcgcagcc atgtcgacca ccggggccag ccgcgcggcg cggctttgcg
    12301 ccatggctta cgcgcctgct gcggcggggt taaaggttgc cgccagctgc gcgctgctgc
    12361 tttccaggct ttcgttgtcc tgcaggtttt gccgtaggta tttgaccatg gccggctggc
    12421 g
//
LOCUS       DASRUM010000311         7826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1833859, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000311 DASRUM010000000
VERSION     DASRUM010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 7826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7826
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833859"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VFF22_20850"
     CDS             complement(<1..551)
                     /locus_tag="VFF22_20850"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HZX19303.1"
                     /translation="MERAIQQVAQQLKQAERILLITGAGLSADSGLPTYRGLGGLYNG
                     HTAEGLPIEEALSGSMLQRDPALCWKYLAELGKACLGAQANAGHYAIAELQRKKPECW
                     LLTQNIDGYHRAAGSPMERVIEIHGELAPLYCQSCGAVDAELAEHLERPLPPKCRQCA
                     GVLRPPVVLFEEMLPEQAIDTLYT"
     gene            complement(562..1233)
                     /locus_tag="VFF22_20855"
     CDS             complement(562..1233)
                     /locus_tag="VFF22_20855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HZX19304.1"
                     /translation="MRDYKWLNEYCLNRFGSVAELEAHLPQPRSAAELRAISDDRYLS
                     TLSLRVFRAGLKHSLVDAKWPAFEQVFFGFDPEKVVLMGAERLENLMQDARIIRHLGK
                     LKSVPRNAQFILDVQKEKGSFGALIADWPVGDIVGLWKYLAKHGSQLGGLSAPRFLRM
                     VGKDTFVPSDDMVAALKAQKIIDKAPTSLKELAAVQAAFNQWQEQSGRPLCQLSVMLA
                     HTVNH"
     gene            complement(1265..2089)
                     /gene="ttcA"
                     /locus_tag="VFF22_20860"
     CDS             complement(1265..2089)
                     /gene="ttcA"
                     /locus_tag="VFF22_20860"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HZX19305.1"
                     /translation="MGTLSVNQNKLQKRLRRQAGEAVADFNMIEDGDKVMVCLSGGKD
                     SYTMLDVLLHLQKVAPIKFEIVAVNMDQKQPGFPEHVLPAYLESIGVPYHIVEKDTYS
                     VVKEKIPEGKTTCSLCSRLRRGTLYTFADEIGATKMALGHHRDDILETFFLNMFYGGT
                     LKAMPPKLRADDGRNVVIRPLAYCNEADIEAYSQLKEFPIIPCNLCGSQENLQRQVVK
                     EMLQDWERKSPGRTEIMFRALQNVVPSQLADRNLFDFKSLKIDDSATPRFLDVMNL"
     gene            2187..2789
                     /locus_tag="VFF22_20865"
     CDS             2187..2789
                     /locus_tag="VFF22_20865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-J related domain-containing protein"
                     /protein_id="HZX19306.1"
                     /translation="MNDDFDPGLDLPEQLHQLLRAAPAGLGEYQLIQLLKARQSVHIP
                     NLPLTDKLVLFRTHFLLFNALYRLREQLWASQSGYLQISALNIQLLPYQPGSSELSEH
                     DPLRDYYLDLSQLSDTDEQDVAKLLLSFWTRMHGSEEKQAALELFELEGREQTLNLAT
                     IKHRYRQLVSLHHPDRGGSTARLQSINKAMEILERYYGRV"
     gene            2948..3547
                     /locus_tag="VFF22_20870"
     CDS             2948..3547
                     /locus_tag="VFF22_20870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HZX19307.1"
                     /translation="MIHHVWGLFTHPDQEWQEIRGEEETISHMYLTHVLILAAIPAVS
                     AYIGTTQVGWAVGNRAPVMLTEASALQMTVMTYLAMLAGIAVMGAFIHWMARTYDASP
                     NLTQCVVFAAYTATPLFIGGLAALYPHLWLAMVVGTAAICYTVYLLYVGIPTFMGIPE
                     DEGFMFSSSVLAVGLVVLVAMIAASVILWGFGVGPVYTS"
     gene            3702..4364
                     /locus_tag="VFF22_20875"
     CDS             3702..4364
                     /locus_tag="VFF22_20875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HZX19308.1"
                     /translation="MFANFYALITRPDQAWAAIRQDEEHNSKSYLPYLLLFSLLPAVC
                     MFIGTYWVGWSLVDQERVRLDLPSALQLSVLLYLTIIIGTAIMGFFLRWMSRTFDARP
                     TLNQCIGFMAYTCTPFFLAGLGALYPSRWLAITVLLIAGALASYLLYIGLPRFMRISH
                     YQGFLYAASTWAIALLVVVTIMVSMILFWTYTLEPSYERSVQQDQGYSPREERALEPS
                     RE"
     gene            4429..4923
                     /locus_tag="VFF22_20880"
     CDS             4429..4923
                     /locus_tag="VFF22_20880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HZX19309.1"
                     /translation="MPEQLYARVEDCYQQAEVFFKRPFARAEVSFKLRGQKAGVAHLT
                     ENKLRFNPQLYRENREDFLKQTVPHEVAHLIAHQLFGLKIQPHGEEWQLIMRGVYELP
                     PNRCHSYAVQRRTVSRYIYRCSCPQGEFPFSAQRHTLVSKGRRYYCRRCKVTLSYTGE
                     QRVE"
     gene            5181..6185
                     /gene="dusA"
                     /locus_tag="VFF22_20885"
     CDS             5181..6185
                     /gene="dusA"
                     /locus_tag="VFF22_20885"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HZX19310.1"
                     /translation="MLHAQTPATTPAKVSRRFSVAPMMDWTDRHCRFFLRQLSQHALL
                     YTEMVTTGAILHGDAARFLRHSVAEYPLALQLGGSVPADLAACAKLAQESGYNEVNLN
                     VGCPSDRVQNNMIGACLMGHPQLVADCVKAMQDAVDIPVTVKHRIGINGRDSYAELCD
                     FVGTVRDAGCRSFTVHARIAILEGLSPKENREVPPLRYEVATQLKQDFPELEIILNGG
                     IKTLEDCQQHLQTFDGVMLGREAYHNPYLLAQVDQLLFGSTRAVMSRFEAMQSIRGYI
                     AAHLAEGGSMHHITRHTLGLAQGFNGARRFRQLLSVDIHKTSEPLALFDQAAQLLQGR
                     "
     gene            6227..7153
                     /gene="tal"
                     /locus_tag="VFF22_20890"
     CDS             6227..7153
                     /gene="tal"
                     /locus_tag="VFF22_20890"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012053145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HZX19311.1"
                     /translation="MTSKLDQLKQFTTVVADTGDLDAIARLKPVDATTNPSLLLKAAA
                     MPGYSQLLKSAVAASNGDLGLACDHFGVAVGGEILKIIPGRISTEVDARLSFDTQATL
                     RRAERLIGLYEEAGIGRERVLIKIASTWEGIRAAEQLEKAGVQCNLTLLFTFAQAQAC
                     ADAGVFLISPFVGRIYDWYKKAESRDFVGAEDPGVQSVTRIYNYYKANAYQTVVMGAS
                     FRNIGQIEQLAGCDRLTISPELLQQLAYDQQPLARLLSPGKAAEAKQSLSESQFRWAM
                     NEDAMGTEKLAEGIRLFARDQEKLEKLLTAQA"
     gene            complement(7220..7702)
                     /gene="rssC"
                     /locus_tag="VFF22_20895"
     CDS             complement(7220..7702)
                     /gene="rssC"
                     /locus_tag="VFF22_20895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist RssC"
                     /protein_id="HZX19312.1"
                     /translation="MSPGRIQFAEQDGTFILKFVGEVRLTLCSALDSTIEKIFTALNF
                     SAIVIDLTETRSIDSTTLGLLAKLSILSRQKIGLLPTVVTTHADITRLLQSMGFDQVF
                     NIVDTPVPCPSCLDDLPSQDQSEELVRAKVLEAHRILMGLNDSNREAFHDLVNALEHH
                     "
     gene            complement(7711..>7826)
                     /locus_tag="VFF22_20900"
     CDS             complement(7711..>7826)
                     /locus_tag="VFF22_20900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX19313.1"
                     /translation="ISSAGGTLDGLRKAFGLANLGDMPDDIALLVLSRNLA"
BASE COUNT         1566 a         2439 c         2246 g         1575 t
ORIGIN      
        1 gtatagagcg tgtcgatggc ctgctcgggg agcatttcct cgaacagcac cacgggcgga
       61 cgcagcaccc cggcgcactg ccggcatttg ggtggcagcg gccgctcgag atgttctgcc
      121 agttcggcat cgacagcccc acaggactgg caatacagcg gcgccagctc accatgaatc
      181 tcgatcaccc gctccatcgg gctacctgcg gcgcggtggt agccgtcgat attctgggtc
      241 agcagccagc actctggctt cttgcgctgc aattcggcga tggcgtaatg cccggcattg
      301 gcctgagcac ccaggcaagc cttgcccagc tccgccaggt atttccagca cagcgccggg
      361 tcacgctgca gcatcgaacc ggacaaggct tcttcaatcg gcaagccctc ggcggtgtgg
      421 ccgttataca ggccaccgag gccgcgatag gtgggcaaac cggaatcggc ggacagcccg
      481 gcaccggtaa tcagcagaat gcgttcagcc tgcttgagct gctgtgcgac ctgctggatt
      541 gcccgttcca tcgccaagcc ctcagtggtt gaccgtatgc gccagcatca ccgacagctg
      601 gcacaacggc cgtccgctct gctcctgcca ttggttgaat gccgcctgca cagcggccag
      661 ctctttcagg ctggtcggcg ccttgtcgat gatcttctgc gccttcaggg cggccaccat
      721 gtcatcgctg gggacgaagg tgtccttacc gaccatacgc agaaagcgcg gcgcggacag
      781 gccaccgagc tggctaccgt gcttggccag gtacttccac aggccaacga tatcgcccac
      841 cggccagtcg gcgatcagcg cgccaaagct gcccttctcc ttctgcacat cgaggatgaa
      901 ctgggcgtta cgcggcacgc tcttgagctt gcccaggtgt cggatgatgc gcgcatcctg
      961 catcaggttt tccagacgct cggcgcccat cagtacgact ttctccggat caaagccgaa
     1021 gaacacctgc tcgaaggccg gccacttggc gtccaccagg ctgtgtttga ggccggcacg
     1081 aaacacccgc aggctgagtg tcgacagata gcggtcatcg ctgatcgcgc gcaactcggc
     1141 agcgctgcgc ggctgcggca gatgcgcttc cagctcggcg accgaaccga agcggttcag
     1201 gcagtactcg ttaagccatt tgtaatcgcg catcggacag tccttgagcc gggaaaccgc
     1261 tgagtcagag gttcatcaca tcgagaaagc gcggggtcgc gctgtcgtcg atcttcaggc
     1321 tcttgaagtc gaacagattg cggtcagcca gctgcgacgg caccacgttc tgcagggcgc
     1381 ggaacatgat ctcggtgcgc cccggcgact tgcgctccca gtcctgcagc atctccttga
     1441 ccacctggcg ctgcaggttt tcctgggagc cacagaggtt gcacggaatg atcgggaatt
     1501 ccttgagctg gctgtaagcc tcaatatccg cctcgttgca gtaagccagc gggcgaatca
     1561 ccacgttacg cccgtcgtcg gcgcgcagct tgggcggcat ggccttgagg gtgccgccgt
     1621 agaacatatt gaggaaaaag gtttccagaa tatcgtcgcg atgatgcccc agggccatct
     1681 tggtcgcgcc gatctcatca gcgaaggtgt acagcgtgcc gcgacgcaga cgcgagcaca
     1741 gcgagcaggt ggttttgcct tccgggatct tctccttgac caccgaatag gtgtccttct
     1801 cgacgatgtg gtacggcaca ccgatggact ccagataggc gggcagcaca tgctccggga
     1861 agccgggctg cttctggtcc atgttcaccg caacgatctc gaacttgatc ggcgcgactt
     1921 tctgcaggtg cagcagcacg tcgagcatgg tgtagctgtc cttgccgccg gacaggcaga
     1981 ccatgacctt gtcgccatcc tcgatcatgt tgaagtcggc aacggcttcg ccggcctggc
     2041 gacgcaggcg tttttgcagt ttgttctgat tgaccgagag ggtgcccatg gtgcgctgga
     2101 atccggaagt cgttgaggat gcggcatttt acgcagaaag cgtggcacgc cctaccccaa
     2161 tcaaagtgaa atgctaggct gcgccgatga acgacgattt cgacccgggc ctcgacctgc
     2221 ccgaacaact gcaccagctg ctgcgcgccg cccctgccgg ccttggtgag taccagctga
     2281 tccagctact gaaagcccgc cagtccgtgc atattcccaa tctgccgctg accgacaagc
     2341 tagtgctgtt tcgcacgcat tttctgctgt tcaacgccct ctaccgcctg cgcgagcagc
     2401 tgtgggcgag ccagagcggc tatctgcaga tcagtgcgct gaatatccag ctgctgccct
     2461 atcagcccgg cagcagcgag ctgagcgagc acgacccact acgcgactat tacctcgatc
     2521 tcagccaact cagtgatacc gacgagcagg atgtggccaa actgctgctg agcttctgga
     2581 cgcgcatgca cggtagcgaa gaaaaacagg cggcgctgga actgttcgag ctcgaaggcc
     2641 gtgagcagac gctcaatctc gccactatca agcaccgcta ccggcagctg gtgagcctgc
     2701 atcaccctga tcgcggcggc agcacggcgc gcctgcaatc gatcaataag gccatggaaa
     2761 tcctagaacg ctattacggg cgcgtttgag agtccgattt gctctaacgc cgcgcctttg
     2821 cccttattaa tggctgccta tactgcgaca tacggtcgca taggttcggc cttgtacctg
     2881 ataacgctgc acacgcgaat caggcgctgc gctggggggc gaccgctata acaataggag
     2941 gtctggcatg atccatcacg tttgggggct attcacccat cctgaccaag aatggcagga
     3001 aatccgtggc gaagaagaaa ccatcagcca catgtatctc acccacgtac tgattctcgc
     3061 ggcaattccc gctgtctcgg cctatatcgg caccacccag gttggctggg cagtcggcaa
     3121 tcgagcgccg gtgatgctga ccgaggccag tgcgttgcaa atgaccgtca tgacctacct
     3181 ggcgatgctc gccgggatcg cagtgatggg cgcgtttatc cactggatgg cacgcaccta
     3241 cgatgccagc ccaaacctta cgcaatgcgt ggtatttgcg gcctataccg ctacaccgtt
     3301 gtttatcggc gggctggcgg cgctctatcc acatctctgg ctggccatgg tggtgggcac
     3361 ggcggccatc tgttacacgg tttatctact ctacgtcggt ataccgacct ttatgggcat
     3421 acccgaggat gaaggcttta tgttctccag ctcggtgctc gccgtaggtc tagtggtact
     3481 ggtggcgatg atcgccgcct cggtgattct gtggggcttt ggggtcggcc ctgtctacac
     3541 cagttgagtg cttagctatg gctatcaggc cgcctccggg cggccttttc aatggcgcca
     3601 actccagctg gtatttgggt tgaacccctg gggagatgct ggttcaaaaa ttctgtgagg
     3661 cagcggaaag gccgcgccgg cgacaacaga tggtggtccc catgtttgcg aatttctacg
     3721 ccctgatcac acgccctgac caagcctggg cggccattcg ccaagatgaa gaacacaaca
     3781 gtaaaagtta cctcccctat ctgctgctgt tcagcctact gcctgcggta tgcatgttta
     3841 tcggtaccta ctgggtcgga tggagcttgg tggaccagga acgtgtgcgc ttggacctgc
     3901 ccagtgcctt acagctaagc gtgctgcttt atctgacaat catcatcggc accgccatca
     3961 tgggcttttt cctgcgttgg atgtcacgca ccttcgacgc caggccgacg ctcaaccagt
     4021 gcatcggttt tatggcctac acctgcacgc ccttcttcct tgctggtcta ggggcgctct
     4081 atccaagccg ctggttggcc atcacggtcc tgctgattgc cggtgcactc gccagttacc
     4141 tgctgtacat cggcctgccg cgctttatgc gcatcagcca ctaccagggc tttctctacg
     4201 cagcctcgac ctgggcaatt gctttgctcg tagtggtcac catcatggtc agcatgatcc
     4261 tgttctggac ctacacactc gagccctctt acgagcgcag cgtgcagcaa gaccaaggtt
     4321 acagccctcg agaggaacgt gcgcttgaac ctagccgcga gtgacgactg cacaggggcc
     4381 acgcttgcgg cataatcgcc acccccgcga ttgtcagcac ctctaaccat gcccgaacaa
     4441 ctctacgccc gtgtcgagga ttgttatcaa caggccgaag ttttctttaa gcgccccttt
     4501 gcccgcgccg aggtgagctt caagctgcgt ggacaaaagg ctggcgtggc gcacctgacg
     4561 gaaaacaagc tgcgcttcaa cccacagctg taccgagaaa accgcgaaga cttcctcaaa
     4621 caaacggtac cccacgaggt tgcccacctg atcgcccatc agctgttcgg cctgaaaatc
     4681 caaccccatg gcgaggaatg gcagctgatc atgcgcggcg tatatgagct accgcccaat
     4741 cgctgccaca gctatgccgt gcagcggcgt acggtcagcc gctatatcta ccgttgcagc
     4801 tgcccacagg gtgagttccc cttctctgcc cagcgccata ccctggtcag caaaggccgg
     4861 cgctactact gccggcgttg caaggtgaca ttgagttata ccggtgaaca gcgcgtggaa
     4921 taagaccatg cccctcggaa ccttcttgcg gacgaatagc cgcctctcca tgtaaactcg
     4981 ccacccatct ttttgattcg aatctgggcg gtgtaactga tcatgccgtc agcctctttt
     5041 gcggtctgat tgttgcctgg tggtacatcc tgttggccgg cggtacatgc gagcggctga
     5101 gtggtacagc gtaccacccg gcttttgaaa cacccgtaca gccccggaaa cacgccagaa
     5161 acgcgcacac cggagccccc atgctgcatg cccaaacccc agcaaccacg cctgctaagg
     5221 tgtctcgccg cttctctgtt gcgccgatga tggattggac tgaccgtcac tgccgcttct
     5281 tcctgcgcca gctgtcgcag cacgccctgc tctacaccga gatggtcacc accggggcca
     5341 tcctgcatgg cgatgcggcc cgttttctgc gccacagcgt ggcggaatat ccgctggccc
     5401 tgcagctggg tggcagcgtt ccggcggatc tggcggcctg tgccaagctg gcgcaagagt
     5461 cgggctataa cgaggtcaac ctcaacgttg gctgccccag cgaccgggtg cagaacaaca
     5521 tgatcggcgc ctgcctgatg gggcatccgc agctggtcgc cgattgcgtg aaagccatgc
     5581 aggatgcggt ggatatccca gtaacagtaa agcaccgcat tggcatcaac ggccgcgaca
     5641 gctatgccga gctgtgcgat ttcgtcggca ccgtgcgcga tgccggctgc cgcagcttta
     5701 ccgtgcatgc gcgcatcgcg attcttgaag ggctctcacc caaggaaaac cgcgaggtac
     5761 cgccgctgcg ctatgaggtg gcaacgcaac tcaaacaaga cttcccggag ctggaaatca
     5821 tcctcaatgg cggcatcaag accctggaag actgccagca gcatctgcag actttcgacg
     5881 gcgtgatgct gggtcgcgag gcgtaccaca acccttatct gttggcccaa gtggaccaac
     5941 tgctgttcgg ctccactcgc gcagtgatga gccgctttga ggccatgcag agcatccgcg
     6001 gctatattgc cgcgcacctc gccgaaggcg gcagcatgca ccatattacc cgccatacgc
     6061 tcggcctggc ccagggcttc aatggcgcgc gacgctttcg ccaactgctc tcggtagaca
     6121 ttcacaagac tagcgagccc ctggcgctat tcgatcaagc ggcgcaattg ctgcaagggc
     6181 gctgactcgg gtaaggtcag gacatctgca acgacaaggc tgtgtcatga cctcaaagct
     6241 ggatcaactc aagcagttca ccactgtggt cgccgatacc ggcgacctcg acgccatcgc
     6301 ccgcctgaag ccggtcgatg ccaccaccaa cccgtccctg ctgctcaaag ccgctgccat
     6361 gcctggttac agtcaactgc tgaaaagcgc agtggctgcc agcaacggcg acctaggcct
     6421 ggcctgcgac cactttggcg tcgcggtggg tggtgagatc ctcaagatca tccccggacg
     6481 gatttccacc gaggtggatg cgcgcctgtc tttcgacacc caggccactc tgcgccgcgc
     6541 cgagcgcctg attggcctgt atgaagaggc cggtattggt cgcgagcgcg tgctgatcaa
     6601 gatcgcctcg acctgggaag gtattcgcgc cgccgagcag ctggaaaagg ctggcgtgca
     6661 gtgcaacctg accctgctgt tcaccttcgc tcaggcccag gcctgtgccg atgccggcgt
     6721 atttctgatt tcgcccttcg tcgggcgcat ctacgactgg tacaagaaag ccgagagccg
     6781 tgattttgtc ggtgcagaag accccggcgt gcagtcggtt acgcgcatct acaactacta
     6841 caaggccaat gcctaccaga cggtggtgat gggtgcgagt ttccgcaata ttggccagat
     6901 cgaacaactg gccggctgcg accgcctgac catcagcccc gagctgctgc agcaactggc
     6961 ctatgatcag cagccactgg cgcgcctgct cagccctggc aaggctgccg aagccaagca
     7021 gagcctgagc gaaagccagt tccgctgggc catgaatgaa gatgccatgg gcaccgaaaa
     7081 gctcgccgaa ggcattcgac tgtttgcccg cgatcaggaa aagctggaaa agctgctaac
     7141 tgcccaggcc tgaatggcct gaatggcctg aatgcaagcg gggcgctcaa ttagcgcccc
     7201 gcttgtttag ccactcgact cagtgatgtt cgagggcatt gaccagatcg tggaaggctt
     7261 cgcgattgga gtcgttcaac cccatcagaa tgcgatgcgc ttccagcacc ttggcgcgca
     7321 ccagctcttc tgattgatcc tgcgacggca ggtcatccag acaactcggg cacggaacgg
     7381 gtgtgtcgac gatattgaag acctgatcga accccatcga ctgcaacagt cgcgtgatat
     7441 cggcgtgggt ggtcaccacc gtcggcaaca gaccgatctt ctgccgcgag agaatcgaca
     7501 gcttggccag cagcccaagg gtcgtgctgt cgatactgcg ggtttcggtg agatcaatca
     7561 cgatggctga gaaattcagc gcggtgaaga ttttctcgat ggtcgaatcc agggccgaac
     7621 acagcgtcag gcgcacttca ccgacaaact tcaagatgaa ggtgccatct tgctcggcaa
     7681 actgaattct accggggctt atcccagggt tcatgcaagg ttcctgctta acaccagcaa
     7741 ggcgatatca tccggcatat cccccagatt ggccagccca aaagccttgc gcaagccatc
     7801 cagggtgccg cccgcggaac tgatca
//
LOCUS       DASRUM010000312         8656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1837789, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000312 DASRUM010000000
VERSION     DASRUM010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8656
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1837789"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VFF22_20905"
     CDS             <1..390
                     /locus_tag="VFF22_20905"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HZX19314.1"
                     /translation="DLFSSLDSFQALSVAASALLVRDVAGQYRPAEVDEVLLAAQQLL
                     AAQVRGSDLMDSPAVVKDFLRARLGNLPHEVFAVVHLDAQNRVVDYVEMFRGSVSQTS
                     VYPREVVRDALLRNSCALVLVHNHPLC"
     gene            complement(474..896)
                     /gene="merR"
                     /locus_tag="VFF22_20910"
     CDS             complement(474..896)
                     /gene="merR"
                     /locus_tag="VFF22_20910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HZX19315.1"
                     /translation="MSNEVALTIGQLARAAGVNVETVRFYQRRALLSEPDRPTGSIRR
                     YSDADTERIRFIKAAQRLGFSLDDVQELLRLEDGTHCSQARQLAERKLGEIRSKIGDL
                     QRIEAVLDEMVGRCRAVRGKVSCPLIASVHLAGAAPLT"
     gene            970..1320
                     /gene="merT"
                     /locus_tag="VFF22_20915"
     CDS             970..1320
                     /gene="merT"
                     /locus_tag="VFF22_20915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric ion transporter MerT"
                     /protein_id="HZX19316.1"
                     /translation="MSSHGSGRWALVSGGLAAVLASACCLGPLVLVSVGLGGAWIANL
                     TALEPYRPVFITVALGALVLAYRRVYRPVEQCRPGEVCAVPAVRRGYRIVFWVVVVLV
                     AIALGFPYAAPLFY"
     gene            1333..1617
                     /gene="merP"
                     /locus_tag="VFF22_20920"
     CDS             1333..1617
                     /gene="merP"
                     /locus_tag="VFF22_20920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016715843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HZX19317.1"
                     /translation="MKPRHIAILLAVLVAPAWSATKTVTLSVPGMNCAACPITVKKAL
                     TRVAGVAKVDVSLDRREAVVTFDDSQAAVGQLTGATTDAGYPSTIKEAAR"
     gene            1614..1826
                     /locus_tag="VFF22_20925"
     CDS             1614..1826
                     /locus_tag="VFF22_20925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008017239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HZX19318.1"
                     /translation="MSAVVPQSTLTCPSCGHSKAETMPTDACQFFYECERCHELLRPK
                     PGDCCVYCSYGSVPCPPVQQQVKCCG"
     gene            complement(1884..2300)
                     /locus_tag="VFF22_20930"
     CDS             complement(1884..2300)
                     /locus_tag="VFF22_20930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX19319.1"
                     /translation="MAHTPPHDPTEVAPAQRLRDALAQLTAADGGAAPTAKALCELAG
                     VSRNALYRYHPDILIELHRLQHRRRRTSGPSALALEQLRADNRSLHHQVAMIAALVDH
                     YFCAWQESQTLLERRERELAELRRHVKPKLVSIQHK"
     gene            complement(2278..4194)
                     /locus_tag="VFF22_20935"
     CDS             complement(2278..4194)
                     /locus_tag="VFF22_20935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX19320.1"
                     /translation="MRAAANLLLGSPTPRSRTRAQAPEARAWFLDADWDDPVWVFKPT
                     NALEEAKPVRVDWRFKLPGGQVFVGHRYAALLHSSKQLIALIRSRSLGTGLPQRASTA
                     AGYFNYLRELVRWMDQAGFSRFTDLDATALLQFQRALALRPGTRRPTLAPTTVQKYLY
                     LLTYLYRFRDELDDGLRIDPFPGRSQGDVAGVHDADVRRWPYTPQAVAVALVQGAIDL
                     VNRGATPILQARDVYAEAVGAAKQQGYLTDACTNAATRALRHARVRVPGQPQPILTVG
                     ALTQAIDMLYAACFVVISYLVGPRASEILHLQAGCVQRRGGAAAEEITVIVGAIFKRQ
                     PEYAGRPHEWVAPPVAATAIAVLEALSAGHRAQSGRPHLWLRRGQTPGATEWQRPCPG
                     TLGIVSTRRMRLLLKRFAVWLGLPQHEGRTWQLSTHQGRKTFARFAALRDGSALFALA
                     QHLGHRERAVTDCGYAGSDYRLNQEIETEILEQSTAAWEHMLAAPGLGGRAGAEIVAK
                     RPHFRGARLKQDLKSYARLLVDAGLVLGVCDWGFCVYREEHSACLGNAAGPNPARREP
                     STCGRCKNFVVSQQHRAYWVEQVRRHEALLDEPALPLQTLRIVRERLNEARALIHSID
                     ADNPGTGHGPHTAP"
     gene            complement(4204..5850)
                     /locus_tag="VFF22_20940"
     CDS             complement(4204..5850)
                     /locus_tag="VFF22_20940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX19321.1"
                     /translation="MRRTVIDFGSLGLPADVQGALAEAFWCHFGAMAERCIPVRWFHI
                     LVFERFVRESGAIRCLADVHGGTLLRYVEWLNTQCRRDGQPWTKSSRASAFTTLRKLL
                     QWIERCRPGRVASIEYPFNPFPWRNRDARPHDKMPAGELRALLKACEADIVRIRAGRE
                     AAAAQRIADAGQPGTLGWLLEHIDRQCDGIVPSARDLSRPGQCTVRTTLQRFGGVRQV
                     EPCLYPRAELLLPYYLAIMAHAAGNPEPIAELGRDCLQSLPLLDDRQALVWFKARSHR
                     IQRRTFSSADCFEPPALIEELLAWNERLRPLARVTERDRLFLYKALRGVTALTCGAAK
                     HLVKAFCERHGLPRFSLVSIRPGVLASFYRASGDLRRVKAIANHAHLSTTVRYVQTPE
                     VQAQHRSRMAALQQAFIGHIERAAASASQRRPINPRGYRSPVPGGEVVSMFGFGCKDP
                     FAGAAAGTHPGELCTNFMGCFTCPNAIITPDPPTLARLLQARDHMRAAAALLHPARWQ
                     AFYAPQLRILEEDILPRFASRELAAALPLVAQLPPLPDLR"
     gene            complement(5952..7139)
                     /locus_tag="VFF22_20945"
     CDS             complement(5952..7139)
                     /locus_tag="VFF22_20945"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZX19322.1"
                     /translation="MVQVVRKSALPDPSEVIDRLAPAAARAPAVALREPRPTGFPLLF
                     TAQWQLIEPAVAFLHEHFVQRAHTIDTLRTYAEVLYDWFETLEQNGVRWDGADAVDLV
                     AYRNRMLTQPSAHTGRPYRIATINHRVRGVLRFYDWAVRTHWLVASPLAGTEHNFRVA
                     RQLRPCSADASRPTDHSLFVLRQFEPLPRPLVSEQARELLATLPPPYDLMARWQLYTG
                     LRVSELLRLCVQDVSKPSVRTVSHHTIEVIRKGRKPGYVVAPASLLDETAAYRSGHRR
                     AWLQRAAHRRGRAADPAALFVNARGAHVGKNAYQRVISRTGQACGFKATTHLLRATFA
                     CMLLARLEQLASAGAAINPLMVVKVLMGHERIETTDRYLRAIAVEAGTLREVLDTLLS
                     EVP"
     gene            complement(7146..7397)
                     /locus_tag="VFF22_20950"
     CDS             complement(7146..7397)
                     /locus_tag="VFF22_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX19323.1"
                     /translation="MPASDALALLADHVKLDPTYLPVKDRHSCRWHASTALGDFEILT
                     TGVKWYDTRACAGGGGAIDLAMHVLGLSFVDAVKRLTAR"
     gene            7597..7749
                     /locus_tag="VFF22_20955"
                     /pseudo
     CDS             7597..7749
                     /locus_tag="VFF22_20955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459650.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            7929..8279
                     /locus_tag="VFF22_20960"
     CDS             7929..8279
                     /locus_tag="VFF22_20960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX19324.1"
                     /translation="MNQKKLVPGCVVYAFVHYGSQTKVRPAIVIEVQDDRVYLLLGST
                     KGLVNDQTTVIVSEEVALKSMGLRFHTQFHWGGGGNQWVSVRNVERIIGNTPSDVLQR
                     MGVAWANAKFNRVV"
     gene            complement(8308..8511)
                     /locus_tag="VFF22_20965"
     CDS             complement(8308..8511)
                     /locus_tag="VFF22_20965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX19325.1"
                     /translation="MTTSSKEGGDQLKSYDPQTAEQVKADFDAGKTLPTAKDHDVKVM
                     TKRFEAMGLPSSTFEKICKDAGL"
BASE COUNT         1480 a         2711 c         2939 g         1526 t
ORIGIN      
        1 gatctgtttt cttcccttga ttccttccag gcactctcgg ttgccgcttc cgccttgctg
       61 gtgcgcgatg tcgcgggcca gtaccgtccg gccgaggtcg acgaggtgct gctggcggcg
      121 cagcagttgc tggccgccca ggtgcgcggc agcgacttga tggactctcc cgctgtggtg
      181 aaggactttc tgcgtgcccg gctgggcaat ctccctcatg aggtgtttgc ggtggtgcat
      241 ctcgatgcgc agaaccgcgt ggtggactac gtcgagatgt tccggggctc ggtgagccag
      301 acatcggtgt acccgcggga ggtggtgcgc gatgcgctgc ttcgaaacag ctgcgccctg
      361 gtcctggtgc acaaccaccc cttatgttga tttcaaggtg ttcaccgagg aagcgtgtca
      421 acgcgccaga gatggatggg gaacacagct tcgctccact gcgccgcgcg gaatcacgtc
      481 aatggcgccg cgccagcgag atgaaccgag gcgatcaatg gacagctcac cttgccccga
      541 acggcgcggc agcgtccgac catctcgtcg agcaccgcct cgatgcgttg caggtcgccg
      601 atcttgcttc gaatctcacc gagcttgcgc tcggcgagct gacgcgcctg gctgcaatgg
      661 gttccgtcct ccagccgcag cagttcctgc acgtcatcga gactgaagcc cagccgctgc
      721 gcagccttga tgaaacggat gcgctcggtg tctgcatccg agtagcgccg gatgctaccg
      781 gtggggcgat cgggctctga cagcagagcg cggcgctggt agaaacgcac cgtttcgacg
      841 ttgacccccg ccgcgcgggc gagctggccg atcgtcaatg caacttcgtt cgacatggtt
      901 gactccgtac tcaggtacgg aaatattatt ccagccatgg agcttcgcac acgaacagaa
      961 ggaagtcgca tgtccagcca tggatccggc cgatgggcct tggtcagcgg tgggttggcc
     1021 gccgtactcg catcggcctg ttgcctcggg ccgctggtgc tggtcagcgt gggcctgggc
     1081 ggtgcgtgga tcgccaacct caccgcgctg gagccgtacc gacccgtgtt catcaccgtc
     1141 gcattggggg cactggtgct ggcctatcgg cgcgtatatc ggccagtcga acagtgccgg
     1201 cctggcgagg tgtgcgcggt gccggccgtg cgccgcgggt accgcatcgt gttctgggtg
     1261 gtcgtcgtgc tggtggcgat tgcgctgggc ttcccgtacg cggcgccgtt gttctactga
     1321 aagggcttgt ccatgaaacc tcgccacatc gccatcctcc ttgccgtgct cgtcgcgccg
     1381 gcgtggtctg cgacgaaaac cgtgacactg tcggtgccgg gcatgaactg cgcagcctgc
     1441 ccgatcacgg tgaagaaagc cctcaccagg gtcgcaggcg tggccaaggt cgacgtgagc
     1501 ctggacaggc gcgaagcggt ggtcacgttc gacgacagcc aggccgctgt cgggcagctc
     1561 actggcgcga cgaccgacgc gggctacccg tcgacgatca aggaggcggc acggtgagcg
     1621 ccgttgtgcc gcagtccacg ctgacctgtc cgtcgtgcgg gcactcgaag gccgagacca
     1681 tgccaaccga cgcgtgccag ttcttctacg aatgcgagcg ctgccacgag ctgcttcggc
     1741 cgaaaccggg cgactgctgc gtgtactgct cgtacggctc ggtgccatgc ccgccggtgc
     1801 agcagcaagt gaagtgctgc ggttgatcgt tcgcatgcac ggctgcgcgc ccgcgtcagc
     1861 gctcatggcc tgcgctgccg ggctcacttg tgctggatcg aaaccagctt gggtttgacg
     1921 tggcggcgca gctcagcgag ctcgcgctcg cgacgttcca gcaaggtttg gctctcttgc
     1981 caggcacaga aatagtgatc gaccaaggcg gcgatcatgg ccacttggtg gtgcaggctc
     2041 cggttgtcgg cgcgcagttg ctccaacgcc agtgcggagg ggcccgatgt tcgccgtcga
     2101 cgatgttgca gtcggtgaag ctcgatcagg atgtcggggt ggtagcggta cagcgcgttg
     2161 cgcgagacgc cagcgagttc gcacagcgcc ttggccgtcg gcgcggcacc cccatccgcc
     2221 gccgtcaact gcgcgagggc atcgcgaagc cgctgcgccg gtgcgacctc ggtcggatca
     2281 tggggcggtg tgtgggccat gaccggttcc cgggttgtcc gcgtcgatcg agtggatcaa
     2341 ggcacgggcc tcgttgagcc gctcgcgcac gatgcgcagc gtctgcaacg gcagcgcagg
     2401 ctcgtcgagc agcgcttcgt gccgacggac ctgctcgacc cagtaggcgc gatgctgttg
     2461 cgacaccacg aagttcttgc aacgcccgca ggtggagggc tcacggcgcg ccggattcgg
     2521 tccggcggcg ttgcccaggc aggcgctgtg ttcctcgcgg tagacacaaa atccccagtc
     2581 gcacacgccg agcacgaggc cggcgtccac cagcagccgg gcgtagctct tgaggtcctg
     2641 tttgagccgg gccccccgaa agtgcgggcg tttggccacg atctcggcgc cggcgcgccc
     2701 gcccaggccg ggtgcggcga gcatgtgctc ccaggccgca gtcgattgtt cgaggatctc
     2761 ggtctcgatc tcctggttga gccggtagtc ggagccggcg tagccacagt cggtgacggc
     2821 gcgctcgcga tggcccaggt gctgcgcgag agcgaacaac gccgatccgt cgcgcaatgc
     2881 cgcgaaccgt gcgaaggtct tgcgcccctg gtgcgtggaa agttgccagg ttctgccctc
     2941 atgctgcggc agcccgagcc acaccgcaaa gcgcttgagc agcagtcgca tgcgcctggt
     3001 cgacacgata cccagcgtgc cggggcatgg gcgctgccac tccgtggcac ctggggtctg
     3061 gccacggcgc agccacaaat ggggccgccc agactgagcg cgatgcccgg ccgacaaggc
     3121 ttcgaggacg gcgatggcgg tcgcggccac cggcggtgca acccattcgt gtggccgccc
     3181 ggcgtactcc ggttggcgct tgaaaatcgc accgacgatc acggtgatct cttcggcggc
     3241 ggcgccgcca cggcgctgca cgcagcccgc ctggaggtgc aggatctcac tggcccgcgg
     3301 tccgacgagg tacgagatga cgacgaagca tgccgcgtag agcatgtcga tcgcctgcgt
     3361 gagcgcaccg accgtcagga tcggctgcgg ctggccaggc acccggacac gtgcgtgacg
     3421 taaagcgcgc gttgccgcat tggtgcaggc gtcggtgagg taaccctgct gtttggcggc
     3481 tcctacggcc tcggcataga catccctggc ctgcaggatc ggcgttgcac cacggttgac
     3541 cagatcgatg gcgccctgga ccagcgccac cgcaacggcc tgcggggtgt acggccagcg
     3601 acggacatcg gcgtcgtgca cgccagcgac atcgccttgg ctgcgccccg gaaaagggtc
     3661 gatgcgcaac ccgtcgtcga gttcatcgcg aaagcgatac agatacgtga gcaggtagag
     3721 gtacttctgc accgtcgtgg gggcgagcgt ggggcgccga gttcccggtc tcagggcaag
     3781 cgcgcgctgg aattgcagca gtgctgtcgc gtcgagatcg gtgaagcgag agaagccggc
     3841 ctgatccatc cagcgcacga gttcgcgcag gtagttgaag tagcctgcag ccgtgctcgc
     3901 acgctgcggc aggccggtgc ccagcgagcg actgcggatg agcgcgatga gctgcttgct
     3961 gctgtgcagc agcgctgcat accgatgtcc cacgaaaact tgcccgcccg ggagcttgaa
     4021 gcgccagtca acgcgcaccg gcttggcctc ttcgagcgcg ttcgtcggtt tgaaaaccca
     4081 gaccgggtcg tcccaatcgg cgtcgaggaa ccacgcacgc gcttcgggcg cctgtgcgcg
     4141 ggttcggctc cttggggtcg gactgccgag aagaaggttt gccgctgcgc gcatggggcc
     4201 agctcatcga agatcgggca gcggtggcag ctgtgccacc aagggcaggg cggccgcgag
     4261 ttcgcggctg gcaaagcgag gaaggatgtc ttcttccagg atgcgcaact gcggcgcgta
     4321 gaaggcctgc cagcgcgctg gatgcagcaa ggcggcggca gccctcatgt ggtcacgcgc
     4381 ctggagcagc cgagccagcg tcggcggatc cggcgtgatg atggcgtttg gacaggtgaa
     4441 gcagcccatg aaattcgtgc acagttcacc ggggtgcgtc cccgccgccg caccggcgaa
     4501 ggggtccttg cagccgaagc cgaacatcga cacgacttcg ccaccgggca ccggtgagcg
     4561 gtagcccctg ggattgatgg ggcgacgctg ggatgcgctg gctgcggcgc gctcgatgtg
     4621 cccgatgaag gcctgttgga gggcggccat gcgagagcgg tgctgcgcct ggacctcggg
     4681 cgtctggacg tagcgcacgg tggtggacaa gtgcgcatgg ttggcgatcg ccttgacgcg
     4741 gcgcaggtcg cccgaggcgc ggtagaagct cgcgagcacc ccgggacgga tcgacacgag
     4801 agagaaccgc ggcaggccgt gccgctcgca aaatgccttg accaggtgtt tggctgcgcc
     4861 acaagtgagt gccgtcacgc cgcgcagggc tttgtagagg aacaggcgat cacgctcggt
     4921 cacgcgcgcc aggggtcgca gccgttcgtt ccacgcgagc agttcctcaa tgagcgctgg
     4981 cggttcgaag cagtcggcgc tgctgaacgt gcgccgctgg atgcggtggg agcgggcctt
     5041 gaaccacacg agcgcctggc ggtcatcgag cagtggcagg gactgcaggc agtcgcgacc
     5101 gagttcggcg atcggttcgg ggttgcccgc tgcgtgagcc atgatggcca ggtagtacgg
     5161 taacagcagt tcggcgcgcg gatagaggca cggttcgacc tgcctcacgc caccgaagcg
     5221 ctggagggtc gtccgcacgg tgcactgccc gggtcgcgac aggtcgcgcg cgctgggcac
     5281 gatgccatcg cattgccgat cgatgtgttc gagcagccag ccgagggttc ccggctggcc
     5341 ggcgtcggcg atgcgttgcg ccgcagcggc ctcacggcca gcacggatgc gaacaatgtc
     5401 tgcttcgcag gccttcagca acgcccgcag ttcgcccgca ggcatcttgt cgtggggtcg
     5461 ggcatcgcgg ttgcgccagg ggaacgggtt gaatgggtac tcgatgctcg caacacgccc
     5521 ggggcggcaa cgctcgatcc actgcagcag tttgcgcagg gtcgtgaacg cgctcgcccg
     5581 actcgacttg gtccacggct ggccatctcg ccggcactgg gtgttcaacc actcgacgta
     5641 gcgcaggagc gtgccaccat gaacgtcggc caggcacctg atggcgccgg attcgcgaac
     5701 gaagcgctcg aagaccagga tgtggaacca gcgcacgggg atacagcgct cggccatcgc
     5761 gccaaagtgg caccagaagg cctcggccag agcaccctgc acgtccgcgg gcaggccgag
     5821 cgagccgaag tcgatgacgg tgcgtcgcac ctggcgaggc gcctcgaacc gagcgggcgc
     5881 tgcgccgccc tcaacgagat cggcgctgtg cgtgaacccg cgaggcattg ggcggcggcg
     5941 agtgcccacg gtcacggcac ctccgacaac agcgtgtcca gcacttctct gagcgtgccg
     6001 gcctccacgg caatggcgcg caggtagcga tcggtggtct cgatgcgctc gtgtcccatg
     6061 agcaccttga cgaccatcag cgggttgatc gcggcgcccg cgctggcgag ctgctccagg
     6121 cgagccagca gcatgcacgc gaaggtcgcc cgcagcaggt gcgtggtcgc cttgaatccg
     6181 caggcctggc cggttcggct gatgacgcgc tgataggcgt tcttgccaac gtgcgcaccg
     6241 cgcgcgttga cgaacaaggc ggcgggatcg gcagcgcgtc ctcggcggtg ggcggcacgc
     6301 tgcaaccagg cccgccggtg cccggagcga tacgctgccg tctcgtcgag caggctcgcc
     6361 ggggcgacca cgtagccggg cttgcggccc ttgcggatca cttcgatggt gtgatgcgac
     6421 accgtccgta ctgaaggctt gctgacgtcc tggacgcaca ggcgcagcaa ttcgctcacg
     6481 cgcagccccg tatagagctg ccagcgcgcc atcaggtcgt acggcggtgg caacgtggcc
     6541 agtagttccc gcgcctgctc cgacacgagc ggacgcggca acggctcgaa ctggcgcagc
     6601 acgaacagac tgtgatcggt tggccggctc gcatcggcgc tgcaggggcg cagctgcctt
     6661 gctacgcgga agttgtgctc cgtgccggcc aagggggacg ccaccaacca atgcgtgcgc
     6721 accgcccagt cgtaaaaccg cagcacgccg cgcacacggt ggttgatggt ggcgatgcga
     6781 tacggtctgc cggtgtgcgc gctcggctgc gtgagcatcc ggttgcgata ggccaccagg
     6841 tccacggcgt cagcgccatc ccaccgaaca ccgttctgct ccagggtctc gaaccagtcg
     6901 tagagcacct cggcataggt cctgagcgta tcgatcgtgt gggcgcgctg cacgaagtgt
     6961 tcgtgcagga aagcgaccgc cggctcgatc agctgccact gcgccgtgaa caggagcggg
     7021 aagcccgtcg gccttggttc gcgcagcgca acggcaggcg ctcgcgccgc tgccggcgcc
     7081 aaccgatcga tcacctcgga cggatccggc agtgccgact tgcgaacgac ctggaccacg
     7141 ccgacctacc ttgcggtcag ccgcttgacc gcatcgacaa acgacagacc gagcacatgc
     7201 atcgccagat cgatggcgcc gcctccgcca gcgcaagctc gcgtgtcgta ccacttgacg
     7261 cccgtggtca gtatctcgaa atcgccgagc gcggtgctcg cgtgccaacg gcagctgtga
     7321 cggtccttga cgggcaggta ggttggatca agcttgacgt gatcagccag caaagccaag
     7381 gcatcgctgg ctggcatctg gcgaagccgt gccaacgttg gagcactgaa cgatctgcga
     7441 gccagcatca ccgcgaaccc ccatggagtt gccgcaaaac gcggggcgcg ccgggctact
     7501 cgcctctgaa agaggcttgg gctgctcggg catgcgccag tctatgcagc tctcctgaga
     7561 aagccaatcg tgttgacaca cgcgaatgca acctaacacc catctggaga acttcaaccg
     7621 agccgttcgg acgaagcact cacgcaatct ctgaaatctg ctttgcagct ggtcgatgtg
     7681 cgtgtgatcg accacgtcat cgtgaaccgg agtgcgttct tctcgtttgc caactccggg
     7741 ctcctttagc cgtggacgat ctgcagtgga tgcacgccat tcactgcatc cctgcagaca
     7801 tgaagcgagg tcggaaattc aagaccatcc agtgatggat gggctttctc aaggcccgtg
     7861 ttttgagaaa gcccagccag gctctcctcc atttgttacc aaccttttgg aaccgaagga
     7921 gagacgcgat gaatcagaag aagcttgttc ctggctgtgt cgtctatgca ttcgtgcact
     7981 acggtagcca gaccaaagtg cgaccagcga tagtgatcga agtacaggac gatcgtgtct
     8041 acttgttgct cggctcgacc aagggccttg tcaacgatca gacgaccgtg atcgtctcag
     8101 aagaggttgc gctgaagagc atgggactcc ggtttcatac gcagttccac tggggtggcg
     8161 gtggcaacca atgggtctct gtacgaaacg tagaaaggat catcggcaac acgccgagtg
     8221 atgtacttca gcgtatgggg gttgcgtggg cgaacgccaa gttcaaccgc gtggtctagc
     8281 gggctttgag tgagtcttcg gactcactca aaggcctgcg tccttgcaaa tcttctcgaa
     8341 ggtgctactt ggcaggccca tcgcttcgaa gcgcttggtc atcaccttca cgtcatggtc
     8401 tttggcagtg ggcagcgtct tgccggcgtc gaagtccgct ttgacttgct cagcggtttg
     8461 ggggtcgtag ctcttgagct gatcgccgcc ttctttgctg gaagtggtca tggtgatctc
     8521 ctgagaattg gaaaaaggcc tggttcatcc tagcgcatgc ggcgcatggt tcaacgtgca
     8581 ttttgctgtg catcttctgc agatcaaagt gcattgcacc accctttcaa cacaaccgct
     8641 atgtgcggat atagaa
//
LOCUS       DASRUM010000313        20553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2504148, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000313 DASRUM010000000
VERSION     DASRUM010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 20553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20553
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2504148"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="VFF22_20970"
     CDS             complement(<1..1434)
                     /locus_tag="VFF22_20970"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HZX19326.1"
                     /translation="MIPVILSGGSGSRLWPLSRKQYPKQFLALTGSDTLFQQTIKRLR
                     FTGMQAPVLVCNLEHRFIVQEQLNTQQLETQTILLEPFGRNTAPAVAIAAMQLLAEGR
                     DELLLVLPADHVLDDQSAFQTALQLASSAAEKGEMVLFGIPATRPETGYGYIKSEADP
                     ALPEGIVRVSKFVEKPDETRAREFVASGDYYWNSGMFLFRASRYLEELKKHDADIYDT
                     CLLALERSQIDGCQVTIDAATFACCPDNSIDYTVMEKTQRACVVPLNAGWNDVGSWSS
                     IWDVHPKDQQGNVLSGDVVIHDSHNCLVHGNGKLVSVIGVQDIVVVETKDATMIVHKD
                     RVQDVKAMVNQLDADGRSETQNHCHVYRPWGSYDSVDMGGRFQVKHITVNPGATLSLQ
                     MHHHRAEHWIVVSGTAEVTCDDKVFLLTENQSTYIPMTSVHRLSNPGKIPLEIIEVQS
                     GSYLGEDDIQRLDDVYGRSQTATLSLAA"
     gene            complement(1604..2248)
                     /locus_tag="VFF22_20975"
     CDS             complement(1604..2248)
                     /locus_tag="VFF22_20975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042121 - alginic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate O-acetyltransferase AlgF"
                     /protein_id="HZX19327.1"
                     /translation="MHSTTSTRRMLYACMLALGLSSLTATADEGGLYAPNAPKGSAFI
                     RGYNASNSEQNFSVGNTELSDIAPLASSDFSFLPAGSYSAQVGSNSLPVKLEAEHFYT
                     LVSQVGEPPKLVDEAPFNNKRKALLRVQNLSDSTLSLKTADGKPVVDGVAPDANGQRE
                     VNPVKVNLALFDGERKVSDLKPVTLARGEVVCLYITGSGNALSPVWVKRPARTD"
     gene            complement(2310..3452)
                     /locus_tag="VFF22_20980"
     CDS             complement(2310..3452)
                     /locus_tag="VFF22_20980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate O-acetyltransferase"
                     /protein_id="HZX19328.1"
                     /translation="MTRPLRTLYVALFLGVLCLLFAWSMRGVLSYQLPKQFSVVDGGL
                     AKTFESHYDEHFPVKTLGTSLWAGLDYLLFNEGRPGVQIGQQGWLYSSEEFKAEVDGA
                     QQVRENLALIKGVRNELAQHDVQLLLAIVPSKSRLYPEFVGDHPTSALRQELYPRFLN
                     EMRAADIAAPDLLSALQAGKANGDMFLRTDTHWTPEGADLVAQQLSNALKGSIRLAGE
                     PQLFVTQASPATPYSGDLTRFLPLEPLFGYLLPPPDQLRRSETQAAEPAAGADALFSD
                     SEMPVALVGTSYSANPAWNFAGALRQHLQRDLSNHAEDGQGPLVPMLKYLQSDELKNA
                     PPQLVIWEFPERYLPMANDFSAFDAQWLSTLKQPVGNRRLAASDRR"
     gene            complement(3464..4951)
                     /locus_tag="VFF22_20985"
     CDS             complement(3464..4951)
                     /locus_tag="VFF22_20985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HZX19329.1"
                     /translation="MVFSSNVFLFLFLPIFLGLYYLSSNRYRNLLILIGSYAFYAWWR
                     VDFLLLFVAVTLWNYIFGLRIYQAGVRSKAAQRWVLWGIVGNLATLGYFKYANFGVAN
                     INKVMEAAGLEPFLLTHVILPIGISFYIFQSLSYLIDVYRGDTKPTENLINFAAFIAL
                     FPQLIAGPVLRYKDLADQFTDRTHSLDKFSEGATRFMQGFVKKVFIADSLAPLVDHIF
                     ALQNPSTGDAWLGMLAYTAQLYFDFSGYSDMAIGLGLMMGFRFMENFNQPYISQSITE
                     FWRRWHISLSTWLRDYLYVPLGGNRGGTFNTYRNLFLTMLLGGFWHGANWTFLIWGAW
                     HGMWLAIERALGVNAAPTLINPLKWAFTFLLVMLGWVIFRAENLDVAWRMYSALFSPA
                     MFSSQGWALSELNRASISDLQIATLLLAYAVMAFCGLRQFYRQRAKNAGSGLLPSGGA
                     LLLTPAGASIALPRYLMRGALLLLFCASVLKLSAQSYSPFLYFQF"
     gene            complement(5075..6193)
                     /locus_tag="VFF22_20990"
     CDS             complement(5075..6193)
                     /locus_tag="VFF22_20990"
                     /EC_number="4.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015096742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannuronate-specific alginate lyase"
                     /protein_id="HZX19330.1"
                     /translation="MKSPGLMPRHSWILAPCLLGAALCSQTLLAASLVPPPGYYAPVA
                     VTAKKAKACPATPPPYTDKLVFRSKYEGSGKARATLNVDSEKAFRSKTAAITEMERGV
                     SKQVMQYLRDGQPTQLECALQWLNDWAEADALLSGEYNHTGKSVRKWALGSLASAYLR
                     LKFSESQPLAAYAQQTQRIEAWFITMAEHTVQDWSDLPLKKINNHSYWSAWSVMATAI
                     ATDRRDLFDWAVAQFRVGANQVDADGYLPNELKRSQRALAYHNYSLPPLVMIAAFAQA
                     NGVDLRAENHGALQRLAERVLRGVDDPEEFASKTGEKQDMDDLKKPNKYAWLAPYCVL
                     YACSADAQALQQKMGPFKTFRLGGNVSQVFEPKHAKGS"
     gene            complement(6202..7626)
                     /locus_tag="VFF22_20995"
     CDS             complement(6202..7626)
                     /locus_tag="VFF22_20995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate O-acetyltransferase"
                     /protein_id="HZX19331.1"
                     /translation="MNTQPFKFRQLALACTLLGVAAGAQAEPQYQVQRVGPLCPAAQD
                     PSQYNTKYLSFFTALVQGEGDWLFRSRYDLRTDFGTTPAGYREFKRLRDALKRKGVEL
                     LIVYQPTRGLVNREKLTAEEKAKFNFEQAKQNYLKAIEGFRQAGIWTTDLSPLFDEQG
                     VDTDYYFKADHHWTPAGADRTAKLVAATLKEIPGFEEIPQQQFESNITGLLPKAGTLH
                     KTAAHFCGTTYATEYVPRFETAMQGEADSDSLFGDEADPQIALVGTSNSGPAYNFAGF
                     LQQHSGAEVLNRSVSGGGFDSALLQYMTSQEFHDNPPQILIWEFATHYDLAQQSFYRQ
                     AVPLVDNGCEGRKAVMKNKVTLKPGDNQVLVNGENSQVQNLRGGDYQIDLTFSDPSVH
                     QAQATLWYLTGRRENFKLEQSDRAPTEGRYVFELRNEADWADLNLLALDIHSPENMPA
                     NLSVEATLCKRPSAGDNDLTAHAQ"
     gene            complement(7638..9227)
                     /gene="algG"
                     /locus_tag="VFF22_21000"
     CDS             complement(7638..9227)
                     /gene="algG"
                     /locus_tag="VFF22_21000"
                     /EC_number="5.1.3.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannuronan 5-epimerase AlgG"
                     /protein_id="HZX19332.1"
                     /translation="MTPRPSKLKRATGIALLCSGLLLGALQPAAPWIATAAAGEAQQQ
                     VKPTQNYTVTSAPIAELHLDPPQLPDLSGYTTAAVEAKIKRTPGGKVVVRRMLQQDAL
                     KDFIGGNERIREWVKRQNGMPHAIFIEGGYVTPKDLARSLPDSQFSEVEPGVYLARLP
                     IVVAQGATLHIDKSTKELRLSQERGSFMVNDGQLFFTETKLTAWREADNSPATFRKAD
                     EFRPFLVSWGGSELYIAASVFTSLGYNTSKSYGVSITQYTPGMQARLKRPEPTGWLID
                     SEFVDHWYGFYCYEAENVVIKGNTYRDNIVYGIDPHDRSHGLIIAENSVHGTRKKHGI
                     IISREVDDSWIINNKSYDNKLSGIVIDRNSVNNLVAYNQVYGNHADGITLYESSDNLL
                     WGNQVLNNARHGIRLRNSLNIRLYENVAAANGLSGVYGHIKDLSNTDRNIQLDPFDTE
                     VSMIVVGGTLAGNGSGPLSIDSPLSVELYRVEMLAPSKSTGISFSGILGDKQEEILDL
                     MVRRQLAVLIDPVESQAELQD"
     gene            complement(9240..10685)
                     /locus_tag="VFF22_21005"
     CDS             complement(9240..10685)
                     /locus_tag="VFF22_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019470597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HZX19333.1"
                     /translation="MLSASQAGAVQMDAMHFGTDIKITVQSEEDRDLGTRDGGDANGI
                     ALDLRPWVRAQRGDWSAFAMGQAVMASDIIQTDTLESSADATESDQTDGRTRKKNYLA
                     LREFWVDYAGLTDYPGEHLRLGRQRLHNEDGQWRDTNIEALNWTFDTTLLQASLGAAQ
                     RFSEYRTDIDELAPEDEDRLHLFADLAGQWQPGHWLGVRVHHSRDDGELADPNQPIDA
                     LDKRANGDLTWLGLHAHSDAYNFRNQKRLNYWASLTSLTGERNSLNSSLDDSIAGGQN
                     STDLNAWATDLGLRLRLDPNWQVGAAYAHGSGGGDDDGNGNDNYRQTGLQSNRSTFTG
                     TRSSVQRFGEAFRGELSNLQAVTLFGSWQLADDYDASLVYHHFWRVDEHQQIGRSGIN
                     ARFDDPLADLSPAPQVALVDGEKELGQELDLVVTKYFKQGLLPAAMSEAIDEPAALVR
                     FRGGVFKPGDAYGSDTDSLMHRAFIDVIWKF"
     gene            complement(10739..12124)
                     /gene="algK"
                     /locus_tag="VFF22_21010"
     CDS             complement(10739..12124)
                     /gene="algK"
                     /locus_tag="VFF22_21010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate biosynthesis TPR repeat lipoprotein
                     AlgK"
                     /protein_id="HZX19334.1"
                     /translation="MSNLNARPLATPLLTLTLALGLSACANLPDQRLAVEALKQGDHA
                     TAEQHFRQLAELGYVDAQIGLADLQLASGSAEGLEQAEQTYRSALDGSPRAQARLGKL
                     LARKPAASPAERREAAQLLEQSFAAGEPSSLLPLVMLYLQYPQDYPDMNVQQRLTTWR
                     QQGHTQADLAQIMLYRSQGNYDQHLEEIEQICQGLISQADVCYVELATVYQKQGKTKQ
                     QKALVQRLMAGYHSTAIPAQRVDAVAQVLADAELGKPDESQAQEMLEAIAPDYPAAWV
                     SLARLLYDYPALGDIEQMLAYLEQGRAAALPRAELLLGRLYYEGKLLPQDPQKAEEHL
                     RKAAPSEPSANYFLGQIYLRGYLGDIYPQQALDHLLSAARNGQINADFALAQMYSQGK
                     GIQPDPVNAFVFSQLALPKNTPQANELARSSEQQLSPSERAKAEQLLADERQLRGTAL
                     QAQTTAQVLNP"
     gene            complement(12136..13302)
                     /locus_tag="VFF22_21015"
     CDS             complement(12136..13302)
                     /locus_tag="VFF22_21015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate biosynthesis protein Alg44"
                     /protein_id="HZX19335.1"
                     /translation="MNTAVNVNVVHESEAQRQHARVKIPAQVHYLNKSRERIELQLCD
                     ISAGGFGVIDNQQAMQIGDFHKGRLQFKLDSFNLGIDIEFQVRSVDVDSKRVGCQLHN
                     LKPREQATLRHLISAYLGGELVSAGDLLSTLQRDNFTKARKHANSGTAMSVLGRLKAV
                     TFSLAIFLVGLAAFGFIFKSVYGLYFVTHAQSALVNVPSLQVTMPREGTVQSLIGTDG
                     SVAKGAPIASFSASMLEMLKGHLNDEQLSPANIETLFGKQIKGTLTSPCDCKVARQLV
                     ADGQFASKGDVIFELVPQNTRATVLASFPYRNFAQARIGSRASFVVAGEETPRYGKIV
                     SSSLHEGGLASDIRVVIEPEQTLSSNLAGQPVEVVLDRGPSLDWMIDKALAAGF"
     gene            complement(13337..14824)
                     /gene="alg8"
                     /locus_tag="VFF22_21020"
     CDS             complement(13337..14824)
                     /gene="alg8"
                     /locus_tag="VFF22_21020"
                     /EC_number="2.4.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017148371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannuronan synthase"
                     /protein_id="HZX19336.1"
                     /translation="MQTHTLKSGLNQAAGWLFYLSLLMLLALALPRSVFDPESRDYLL
                     LIGAVGIWRYSMGAVHFVRGMLFLYVVFPHYRRKVAKLGNATDPSQVFLLVTSFRIDA
                     LTTAQVYRSVIREAIDCGYPTTVVCSIVELSDELLVKSLWAQMQPPEHVTLDFVRIPG
                     TGKRDGLAYGFRAISRQMPDTDAVVAVIDGDTVLGEGVVRKTVPWFKLFPNVGGLTTN
                     EFCEVRGSYVMSEWHKLRFAQRHLNMCSMALSKRVLTMTGRMSVFRAEVVTDPAFIAD
                     VESDHLDHWRLGRFQFLTGDDKSSWYSLMRLGYDTFYVPDAAINTVEHPPEKSFIKAS
                     RKLMFRWYGNNLRQNARALRLGVSRLGGFTSLVLFDQRVSMWTSLLGLSVAIIASLKY
                     GLGFILVYLLWIGLTRLILTLLLSVTGHHIGPAYPLILYYNQIVGALVKIYVFFRLDQ
                     QSWTRQNTKLDRGLVSFQRWFNTWSSRAMTFSAASLFVATLMVLV"
     gene            complement(14908..16218)
                     /locus_tag="VFF22_21025"
     CDS             complement(14908..16218)
                     /locus_tag="VFF22_21025"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HZX19337.1"
                     /translation="MRISIFGLGYVGAVCAGCLSARGHQVIGVDVSASKIDLINNGKS
                     PIVEPGLEELLQQGLASGNLRGTTDVIDAVLNSDVSFICVGTPSKKNGDLELNYIEGV
                     CREIGLAMRDKTERHTVVVRSTVLPGTAKNVVLPILEDCSGKQAGVDFGLAVNPEFLR
                     ESTAIKDYDFPPMTVIGELDQASGDLLESIYSELDAPIIRKSIDVAEMIKYTCNVWHA
                     TKVTFANEIGNIAKAAGVDGRDVMEVICQDHKLNLSKYYMKPGFAFGGSCLPKDVRAL
                     SYRAGSMDVDAPLIGSLMRSNAIQVQNAFDIIASYDKRKVALLGLSFKAGTDDLRESP
                     LVELAEMLIGKGYDLSIFDRNVEYARVYGANKDYIESKIPHVSSLLSSDFDSVVDNAD
                     IIVLGNSDMHFAPLAQQAMHGKHVVDLVGFMPTASRAGSEGICW"
     gene            complement(16818..17597)
                     /gene="yaaA"
                     /locus_tag="VFF22_21030"
     CDS             complement(16818..17597)
                     /gene="yaaA"
                     /locus_tag="VFF22_21030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HZX19338.1"
                     /translation="MLMVISPAKTLDYETPPATPRFTQPEHLDHAQDLIQQLRDFTPA
                     QIAELMHLSDKLAGLNAARFGSWERPFNPSNAKQALLAFKGDVYTGLNAEDFSEADFD
                     FAQTHLRMLSGLYGVLRPLDLMQPYRLEMGTKLANPRGNNLYDFWGERISGWFNEALA
                     AQGDEVLLNLASNEYFSAVKRKALNARIIDTEFKDLKNGQYKIISFYAKKARGLMARY
                     VIKERLSDPTGLKDFTYEGYRYSAENSKTDTLVFLRDHPQD"
     gene            17868..18875
                     /gene="dmeF"
                     /locus_tag="VFF22_21035"
     CDS             17868..18875
                     /gene="dmeF"
                     /locus_tag="VFF22_21035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HZX19339.1"
                     /translation="MTACNHSRWEPSHDYRPLETTAERQAWRVAALTGVTMLVEIVAG
                     YAFNSMALLADGWHMASHMLAIGLTALAYLLARRYAHDPRFAFGTWKVEVLAGFASSL
                     MLLVVVAMLAFESFWRLLQPQVIAFEMALWVAVIGLLVNLASAWLLRDSHDHGHAHSH
                     AGVHAHADHDHDHDHDHDHDHPAPAAGKDLNRHAAFIHVLADALTSVAAIVALLGGLL
                     LGWDWLDPLMGVIGAVIIAIWAKGLLIETGKVLLDREMDSPVVARVRSALAQEADTEL
                     ADLHLWRVGRAQFACIVSVVTHGDSSADRYKARLAGIAELVHVTVEVNRCADKHGVER
                     G"
     gene            19177..>20553
                     /locus_tag="VFF22_21040"
     CDS             19177..>20553
                     /locus_tag="VFF22_21040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZX19340.1"
                     /translation="MDDHGRSKPTAPTLYALDTNVLIHDPNALLNFQEHHVAIPMTVL
                     EELDKLKTGKQGVAAECRQAIRLIDKILGSANPEEVEHGVAIQRDKSGPCGFLSILMS
                     KSATPVTWLPEDLNDNKIINQLVELKSRRPGLNVVLVTKDINMLLKARACGIDSEDYH
                     TDQLVDDVSLLSQGYHNMDGSFWDRVNKVETRQDHGRTWHRVQLTDNLPAVHVNEFII
                     DEQGFVGWIKGIKHGELLILDMHQEPLMHQEAWGLRPRDIYQSLALFALLDPDIHLVN
                     LSGAAGSGKTILALAAAIEQTMVSKRYRRIIATRSVQGLDQEIGFLPGTEAEKMEPWL
                     GAITDNLEALHMDDENTHGSVDYILQKVPLQFKSLNYIRGRSFQQSLILIDECQNLTP
                     HQMKTIITRAGSGSKVVCLGNLAQIDTPYLSAPSSGLTYLTERFKDFEHGVHITLQGV
                     PRSILAEYA"
BASE COUNT         3651 a         5911 c         6672 g         4319 t
ORIGIN      
        1 tgcggcgagg ctgagggtgg cggtttggct acggccatag acatcatcca ggcgctggat
       61 atcgtcttcg ccgaggtagc tgcccgactg cacttcgatg atttccaggg ggatcttgcc
      121 cgggttgctc aggcgatgca ccgaggtcat ggggatgtag gtcgactggt tttcggtgag
      181 caggaacacc ttgtcatcgc aggtcacctc ggcagtgccg gacaccacga tccagtgttc
      241 ggcgcggtgg tggtgcatct gcagcgagag ggtggcgccg gggttgaccg tgatgtgctt
      301 gacctggaaa cgcccaccca tgtccaccga atcataagag ccccatgggc gatagacgtg
      361 gcagtggttt tgggtttcgc tgcggccgtc ggcgtcaagc tggttgacca tggccttgac
      421 gtcctgtacg cggtctttgt gcacgatcat ggtggcgtct ttggtttcca ccaccacgat
      481 gtcctgcacg ccgattaccg agaccagttt gccgttgccg tgtaccaggc agttgtggct
      541 gtcgtgtatc accacatcgc cggacagcac gttgccctgt tggtccttgg ggtgtacgtc
      601 ccagatcgag gaccagctgc cgacatcgtt ccagccggca ttcagcggca ccacacaggc
      661 ccgctgggtt ttttccatca ccgtgtagtc gatggagttg tccgggcagc aggcgaaggt
      721 ggcagcatca atggtcacct ggcagccgtc gatctggctg cgctccagcg ccagcaagca
      781 agtgtcatag atgtcggcgt cgtgcttttt cagctcttcg aggtaacggc tggcgcggaa
      841 caggaacatg ccgctgttcc agtagtagtc gccgcttgcc acaaactcac gggcccgagt
      901 ttcatcaggt ttttcaacga atttggagac gcgaacaata ccttctggca gagccgggtc
      961 tgcctctgac ttgatatagc catagccggt ttccgggcgg gtggcgggaa tgccgaacag
     1021 caccatttca cccttctccg ccgcactgct ggccagctgc aaggccgtct ggaaagcgct
     1081 ctgatcgtcc agcacatgat cggcggggag gaccagcaac agttcgtcac ggccttcggc
     1141 gagcaattgc atggcggcga tggctacggc cggcgcggtg ttgcggccaa agggttcaag
     1201 cagaatggtc tgggtttcca gctgctgggt gttgagctgt tcctggacga taaaacggtg
     1261 ttccaggttg cacaccagca ccggagcctg catgccggtg aagcgcaggc gtttgatggt
     1321 ttgctggaac agcgtatcgc tgccggtgag ggcgagaaac tgcttgggat actgcttgcg
     1381 cgagagcggc caaagtcgcg agccgctacc gccggagaga attactggaa tcattgcatg
     1441 tactcctaaa gcacttttgg tttggttggg ctgccggctt gtggccggac tggcccggtt
     1501 acccaggctg gtaagcagcg ctgggggcac tccaaaacgt ctactgcgaa agacgctgct
     1561 ttgctgtgtt ggtcattgcc agcacttgta cttgtccgaa tgttcagtcg gttcttgccg
     1621 ggcgcttgac ccataccggc gagagcgcgt tgccgctgcc ggtgatgtac aggcacacca
     1681 cttcgccgcg ggccagggtg accggcttga ggtcgctgac tttgcgttcg ccatcgaata
     1741 gcgccaggtt gaccttgacc gggttcactt cgcgctggcc attggcgtct ggcgctacgc
     1801 cgtcgaccac gggtttgccg tcggcggttt tcaggctgag ggtgctgtcc gagaggttct
     1861 gtacccgcag cagcgccttg cgcttgttgt tgaagggtgc ttcgtccacc agcttgggtg
     1921 gctcgcccac ctggctgacc agggtgtaga aatgctcagc ttcgagtttt accggcaggc
     1981 tgttgctgcc gacctgggcg ctgtagctgc cggccgggag gaagctgaaa tcactggaag
     2041 ccagcggcgc gatatcgctg agctcggtgt tgccgacgct gaagttctgc tcgctgttgc
     2101 tggcgttgta gccgcggata aaggccgagc ccttgggcgc gtttggcgca tacagaccgc
     2161 cttcgtcagc ggtggcagtg aggctgctga ggcccagtgc gagcatgcag gcatagagca
     2221 tccggcgggt tgaagtggtg ctgtgcatgg gtagaactcc ctgtgggtta acaaaacggt
     2281 agcgagtacc gtggttgcgg cggcaggctt tagcgtctgt cgctggcggc caggcggcgg
     2341 ttgccgacgg gttgtttgag tgtgcttagc cactgcgcat caaaggcgct gaagtcgttg
     2401 gccatcggca ggtagcgttc ggggaactcc cagatcacca gctgcggcgg ggcgttcttc
     2461 agctcatcgc tttgcaggta cttgagcatc ggcaccagcg ggccctggcc gtcttcggcg
     2521 tggttgctga ggtcgcgttg caggtgctgg cgcagcgctc cggcaaaatt ccaggcgggg
     2581 ttggcgctgt aactggtgcc gaccagcgcc accggcatct cgctgtcgct gaacagggcg
     2641 tcggcccctg ctgctggctc ggcggcctgg gtttcgctgc ggcgcagttg atcgggcggc
     2701 ggtagcaggt agccgaacag cggctccaat ggcagaaaac gcgtgaggtc gccgctgtag
     2761 ggcgtcgctg ggctggcctg ggtgacaaac agctgcggct cgcctgccag acgaatactg
     2821 cctttgagcg cattgctcag ttgctgggcg accagatctg cgccttctgg cgtccagtgg
     2881 gtgtcggtgc gcaggaacat gtcgccgttg gctttaccgg cctgcaaggc actgagcaga
     2941 tccggcgcgg cgatatcggc ggcgcgcatt tcgttgagga agcgcgggta aagctcctgg
     3001 cgcagggcac tggtcggatg atcgccgaca aactccgggt acagccgcga cttgctcggc
     3061 acgatggcca gcagcagctg cacgtcgtgt tgcgccagct cattgcgcac gcctttgatc
     3121 agcgccaggt tctcgcgcac ctgctgcgcg ccgtcgactt ccgctttgaa ctcttcactc
     3181 gagtacagcc agccctgctg gccaatctgc acgcctggac ggccttcgtt gaacagcagg
     3241 taatccaggc cagcccacag gctggtgccg agggtcttga ccgggaagtg ctcgtcgtaa
     3301 tggctttcga aggtcttcgc caggccgccg tcaaccacgc tgaactgctt gggtagctga
     3361 tagctcagca ccccgcgcat ggaccaggca aacaacaggc acagcacgcc gagaaacagc
     3421 gcaacataga gggtgcgtaa cgggcgcgtc atggtcggcc tcctcaaaac tggaagtaca
     3481 ggaacggcga atagctttgc gccgagagct tgagcaccga ggcgcagaac agcagcagca
     3541 aggcgccgcg catcaggtaa cggggcaggg cgatgctcgc gccggcaggc gttagcagca
     3601 gcgcgccgcc gctgggcagc aggccgctgc ccgcgttttt cgcgcgctgc cggtaaaact
     3661 ggcgcaggcc gcagaaggcc attaccgcgt aggccagcag cagggtggcg atctgcaggt
     3721 cgctgatgct ggcgcggttc agttcactca gcgcccagcc ttggctgctg aacatcgctg
     3781 ggctgaacag ggcgctgtac atgcgccagg ccacgtccag gttctcggcg cggaagatca
     3841 cccagccgag catcaccagc aggaaggtga aggcccactt cagcgggttg atcagcgtgg
     3901 gtgcggcgtt cacccccagg gcgcgttcga tggccagcca catgccatgc cacgcgcccc
     3961 agatcaggaa cgtccagttg gcgccgtgcc agaagccgcc cagcagcatg gtcagaaaca
     4021 ggttgcggta ggtgttgaag gtgccgccac ggttgccgcc cagcggtacg tagaggtagt
     4081 cgcgcagcca ggtggagagg ctgatatgcc agcgtcgcca gaactcggtg atcgactggc
     4141 tgatataggg ctggttgaag ttttccataa agcggaaacc catcatcagg cccaggccga
     4201 tggccatgtc gctgtagccg gagaagtcga aatacagctg cgcggtgtag gccagcatgc
     4261 ccagccaggc atcgccggta ctggggttct gcagggcgaa gatatggtca accaacggcg
     4321 ccaggctgtc ggcgataaac accttcttga cgaagccctg cataaagcgc gtggcacctt
     4381 cgctgaactt gtcgaggctg tgggtgcggt cggtgaactg gtcggccagg tccttgtagc
     4441 gcagcaccgg gcctgcaatc agctgcggga acagggcaat aaaggcggcg aagttgatca
     4501 gattttctgt gggcttggtg tcgccgcggt acacgtcgat caggtagctg agcgactgga
     4561 agatatagaa ggaaataccg atcggcagga tcacatgggt cagcaggaac ggctccagac
     4621 cggccgcttc catcacctta ttgatgttcg ccacaccgaa gttggcgtac ttgaagtagc
     4681 ccagggtcgc caggttgccg acgatgcccc agagcaccca gcgctgcgcc gccttggacc
     4741 tgacccccgc ctgataaatg cgcaggccga agatgtaatt ccatagggtg acggcgacaa
     4801 acagcaggag gaaatccacc cgccaccagg cgtagaaggc gtagctaccg atcaggatca
     4861 gcaggttgcg atagcgatta ctgctcaggt aatacaggcc gagaaagatc ggcagaaaca
     4921 ggaacaggaa tacgttggat gaaaagacca tccgtagatc tccgttgagt agcaactctt
     4981 ccggggctca cagcccccct ttccccccgc gagcgggaga cttgtttcac cccgtcactg
     5041 gacgggttta cagacgtatc agcgcctggg ctaatcaact gcccttggcg tgtttgggct
     5101 cgaatacctg gctgacgttg ccgccgaggc ggaaggtctt gaacggcccc attttctgct
     5161 gcagcgcctg ggcgtcggcc gagcaggcgt agagcacgca atagggcgca agccaggcgt
     5221 acttgttggg ttttttcagg tcgtccatgt cttgtttctc gccggtcttg ctggcgaatt
     5281 cctctgggtc gtccacgccg cgcagtaccc gttcggccag gcgttgcagt gcgccgtggt
     5341 tctctgcgcg tagatcgacg ccgttggcct gggcgaaggc ggcgatcatc accagcggcg
     5401 gcaggctgta gttgtggtag gccagggcac gctggctgcg cttcagctcg ttgggcaggt
     5461 agccgtcggc gtcgacctgg ttggctccga cgcgaaactg cgccacggcc cagtcgaaca
     5521 ggtcgcggcg atcggtggcg atggccgtgg ccatgaccga ccaggcggac cagtagctgt
     5581 ggttgttgat tttcttcagc ggcaggtcgc tccagtcctg cacggtgtgc tcggccatcg
     5641 tgataaacca ggcttcgatg cgctgggttt gctgcgcata ggccgccagc ggttgtgatt
     5701 ccgaaaactt caggcgcaga taggctgagg ccagactgcc cagtgcccat ttgcgtaccg
     5761 atttgccggt gtggttgtac tcgccgctga gcagggcgtc ggcttctgcc cagtcgttca
     5821 gccactgcag cgcgcattcg agctgcgtcg gttgaccatc gcgcaggtac tgcatgacct
     5881 gtttgctgac cccgcgttcc atctcggtga tggcggcggt tttgctgcga aaggcctttt
     5941 ccgaatcgac attgagggtg gcgcgggcct tgccggagcc ttcgtattta ctgcgaaaca
     6001 ccagcttgtc ggtgtagggc ggcggcgttg ccgggcaggc tttggccttt ttcgcggtaa
     6061 ccgccacggg cgcgtaatag cccggcggcg gcaccaggct ggcggccagc agcgtttggc
     6121 tgcacagggc cgcaccgagt aggcagggcg cgaggatcca actgtggcgt ggcattaagc
     6181 ctgggctttt catgattgtc ttcactgggc atgcgccgtg aggtcgttgt cgccagcgct
     6241 ggggcgcttg cacaacgtgg cctcgacact gagattggcc ggcatgtttt ccgggctgtg
     6301 gatatccaga gcgagcaggt tgaggtcggc ccagtcagcc tcgttgcgca actcgaagac
     6361 ataacgacct tcggtagggg cgcggtcgct ctgttcgagc ttgaagttct cgcgccgccc
     6421 ggtgagatac cagagagtgg cctgggcctg atgcacgctg gggtcgctga aggtcaggtc
     6481 gatctggtaa tcaccgccgc gcaggttctg cacctggctg ttttcgccat tgaccagcac
     6541 ctggttgtcg ccgggtttga gggtgacctt gttcttcatc accgccttgc ggccttcaca
     6601 gccgttgtcc accagcggca cggcctggcg gtagaagctt tgctgcgcca ggtcgtaatg
     6661 ggtggcgaat tcccagatca gaatctgtgg cgggttgtcg tgaaactcct ggctggtcat
     6721 gtactgcagc agcgcgctgt cgaagccgcc gccactgacc gagcggttaa gcacttcggc
     6781 gccgctgtgc tgctgcagga atccggcgaa gttgtaggcc gggccgctgt tgctggtacc
     6841 gaccagggca atctgcgggt cggcttcgtc gccgaacagg ctgtcgctgt cggcctcacc
     6901 ctgcatggcg gtctcgaaac gcggcacata ctcggtggcg taggtggtgc cgcagaaatg
     6961 tgcggcggtt ttgtgcaggg tgccggcctt gggcagcagg ccggtgatgt tgctttcgaa
     7021 ttgctgctgg ggaatctcct cgaaccccgg gatttccttg agcgttgcgg ccaccagctt
     7081 ggcggtacgg tcggctccgg cgggtgtcca gtgatggtcg gccttgaagt agtagtcggt
     7141 gtctacgccc tgttcgtcga acagcgggga caggtcggtg gtccagattc ctgcctggcg
     7201 aaagccttcg atggccttga ggtagttctg cttggcctgc tcgaaattga acttggcttt
     7261 ttcctcggcg gtgagctttt cgcggtttac caagccgcgc gtcggttgat aaacgatcag
     7321 caattccacg cctttgcgct tgagtgcatc gcgcagtcgc ttgaactctc ggtagccagc
     7381 gggcgtagtg ccaaagtcag tgcgcaggtc ataacggctg cgaaacagcc agtcaccttc
     7441 gccctgcacc agggcggtga aaaaactcag gtacttggtg ttgtactggc tggggtcctg
     7501 ggcggcgggg cacagcgggc cgacccgctg tacctgatac tgcggttcgg cctgtgcgcc
     7561 agcggctaca cccagcaggg tgcaggcgag ggccaattgg cggaatttga aaggctgtgt
     7621 gttcacgttg tcgttcctca atcctggagt tctgcctggc tttccacggg gtcgatcagc
     7681 acggccagct ggcggcgcac catcagatcg aggatttctt cctgcttgtc accgaggatg
     7741 ccgctgaagc tgatgccggt gcttttgctc ggtgcgagca tttccacgcg gtacagctca
     7801 accgacagtg gcgagtcgat ggacagcggg ccggaaccgt tgcccgcgag ggtgccgccg
     7861 accacgatca tcgaaacctc ggtgtcgaag gggtcgagct ggatattgcg gtcggtgttg
     7921 gaaagatcct tgatgtgccc gtacaccccg ctcaggccgt tggcggcggc gacgttctcg
     7981 tacaggcgga tattcaggct gttgcgcagg cgaatgccgt ggcgggcatt gttcaggacc
     8041 tggttgcccc acagcaggtt gtccgagctt tcatagaggg tgatgccgtc cgcatggttg
     8101 ccgtacacct ggttgtaggc caccaggttg ttcacgctgt tacggtcgat aacgatgccg
     8161 gagagcttgt tgtcatagct cttgttattg atgatccagc tgtcgtcgac ctcgcgggag
     8221 atgatgatgc cgtgcttctt gcgggtgccg tgcacgctgt tctcggcgat gatcaaaccg
     8281 tgcgagcggt cgtgcgggtc gatgccgtag acgatgttgt cgcggtaggt attgcccttg
     8341 atcaccacgt tttcggcttc atagcagtag aaaccgtacc agtggtcgac gaactccgaa
     8401 tcaatcagcc agccggtcgg ctccgggcgt ttcaggcggg cctgcatgcc gggggtgtac
     8461 tgggtgatgc tgacgccgta ggacttgctg gtgttgtaac ccaggctggt gaagacgctg
     8521 gcggcgatat acagctcgct gccgccccag gacaccagaa acgggcggaa ctcatcggct
     8581 ttgcggaagg tagctgggct gttgtccgct tcacgccagg cggtgagttt ggtttcggtg
     8641 aaaaacagct ggccgtcgtt gaccataaag ctgccgcgct cctgggacag gcgcagctct
     8701 ttggtgcttt tgtcgatatg cagggttgcg ccctgggcca ccacgatggg taggcgtgcc
     8761 aggtagacgc ctggctcaac ttcgctgaac tgactgtccg gcaggctgcg tgctagatcc
     8821 ttgggcgtca cgtagccgcc ttcgataaag atcgcatggg gcatgccgtt ctgccgcttg
     8881 acccactcgc gtatgcgctc attgccgccg ataaagtcct tcagcgcatc ctgctgcagc
     8941 atgcgccgca ccacgacttt gccgcctggt gtgcgcttga tcttggcttc gacggctgcg
     9001 gtggtatagc cgctgaggtc gggcagttgc ggtgggtcca ggtgcagctc ggcgatgggc
     9061 gcgctggtta ccgtgtagtt ctgcgttggt ttgacttgtt gctgcgcttc gccggctgct
     9121 gccgtggcta tccagggcgc ggccggctgc agcgcgccga gcagcaggcc gctgcacagc
     9181 agggcaatgc cggtggcgcg tttgagtttg ctagggcgtg gcgtcatggc gcggggtcct
     9241 tagaatttcc agataacatc gataaaggcc cgatgcataa gcgagtcggt gtcgctgccg
     9301 taggcatcgc caggcttgaa tacgccacca cggaaccgca ccagggccgc cggctcgtcg
     9361 atggcttcgc tcatcgccgc aggcagcagg ccttgcttga agtacttggt aacgaccaga
     9421 tcaagctcct ggccgagctc tttctcaccg tccaccaggg ccacctgcgg ggcgggggat
     9481 aggtcggcga gtgggtcatc gaaacgcgcg ttgataccgc tgcggccgat ctgctggtgc
     9541 tcatcgacgc gccagaaatg gtggtagacc aggctggcgt cgtagtcgtc ggccaactgc
     9601 caggagccga acagggtgac ggcctgcagg ttgctcagct cgccccggaa ggcttcgccg
     9661 aagcgctgca cactgctgcg ggtgccggta aaggtggagc ggttgctctg caggccagtc
     9721 tgccggtagt tgtcgttgcc attgccgtcg tcatcgccgc caccactgcc atgggcgtag
     9781 gccgcgccga cctgccagtt gggatcaagg cgcagacgca gccccaggtc ggtggcccag
     9841 gcgttcaggt cggtgctgtt ctggccgccg gcaatgctgt catccaggct gctgttgagg
     9901 ctattgcgct cgcccgtcag cgaggtaagg ctggcccagt agttcaggcg cttctggttg
     9961 cgaaagttgt acgcgtcact gtgcgcgtgc aggcccagcc aggtgaggtc gccattggcg
    10021 cgtttatcca gtgcgtcgat gggctgattg ggatcagcca gttcgccatc gtcgcgcgag
    10081 tggtgcacgc gcacaccgag ccagtgcccg ggctgccatt gaccggcaag atcagcaaac
    10141 aggtgcaggc gatcctcgtc ttcgggggcc agctcgtcga tgtcggtgcg gtactcactg
    10201 aagcgctggg cggcgcccag gctggcctgc agcagagtgg tgtcgaaggt ccagttcagt
    10261 gcttcgatat tggtatcgcg ccattgcccg tcttcattgt gcagacgttg tcggcccagg
    10321 cgcaggtgtt cgccggggta gtcggtgagg ccggcgtagt cgacccagaa ttcgcgcagt
    10381 gccaggtagt tcttcttgcg ggtgcggcca tcggtctggt cggactccgt tgcgtctgcg
    10441 gacgactcca gggtgtcggt ctggatgatg tcgctggcca ttaccgcctg gcccatggcg
    10501 aaagcactcc agtcaccccg ttgggcgcgc acccaggggc gcaggtcgag ggcgatgccg
    10561 ttggcgtcgc cgccgtcacg ggtgcccagg tcgcggtctt cttccgattg cacggtgatc
    10621 ttgatgtcgg tgccaaaatg catggcgtcc atctgcacgg ccccggcctg gctggcgctg
    10681 agcatggtca gaccaaggcc gaggctgatc gggttgagtt gcagcaatgc acggcgcgtc
    10741 atgggttaag cacctgtgca gtggtttgcg cttgcaaggc tgtgccgcgt agctggcgtt
    10801 catcggcgag taattgctcc gctttggcgc gctcgctggg gctcagttgc tgttcgctgc
    10861 tgcgcgccag ctcgttggcc tgcggggtat tttttggcag ggccagctgg ctgaagacaa
    10921 aggcgttgac cgggtctggc tggatgccct tgccctgcga atacatctgc gcgagggcga
    10981 agtcggcgtt gatctgacca ttgcgtgcgg cgctgagcag gtgatcgagg gcctgttgcg
    11041 ggtagatgtc gccgaggtaa ccgcgcaggt agatctggcc gagaaaatag ttagcgctgg
    11101 gttcgctggg ggcggccttg cgcaggtgct cttcggcttt ctgcgggtct tgcggcagca
    11161 gcttgccttc gtagtacagg cgcccgagca gcagttcggc gcggggcagg gcggcggcgc
    11221 ggccctgttc gagataagcg agcatctgct cgatatcacc cagtgctggg tagtcgtaaa
    11281 gcaggcgcgc caggctcacc caggcagcgg gatagtccgg cgcaatggct tcgagcatct
    11341 cctgcgcctg gctttcatca ggcttgccga gttcggcgtc ggccagtacc tgggccacgg
    11401 catccacccg ttgcgcgggg atcgccgtgc tgtggtaacc ggccatcagg cgctggacca
    11461 gggctttctg ctgtttggtt ttgccttgtt tctggtacac ggtggccagc tcgacatagc
    11521 agacatcggc ctggctgatc aggccctgac agatctgttc gatctcttcc aggtgctggt
    11581 cgtagttgcc ttggctgcgg tacagcatga tctgcgccag atcggcctgg gtatggcctt
    11641 gttggcgcca ggtggtgagg cgttgttgta cgttcatgtc gggataatcc tgtgggtatt
    11701 gcaggtacag catcaccagc ggcagcaggc tgctgggttc ccccgcagcg aaggactgct
    11761 ccagcaattg cgcggcttcg cggcgttcgg cagggctggc ggcgggtttg cgggcgagca
    11821 atttgcccag ccgcgcttgc gcccgtggcg agccatcgag ggcgctgcgg taggtctgct
    11881 cggcttgctc cagaccttct gcggagccgc tggccagttg cagatcggcc aggccgattt
    11941 gcgcgtcgac atagcccagc tcggccaact gacggaaatg ctgctcggcg gtggcgtggt
    12001 cgccctgctt cagcgcttcc acggccaagc gctggtcagg caggttggcg caggcgctca
    12061 ggcccaacgc cagggtgagc gtgagcaggg gcgtggccag cggacgggcg ttgaggttcg
    12121 acatgcgcct gctcctcaga aaccagccgc cagggctttg tcgatcatcc agtcgagcga
    12181 cgggccacgg tcgagcacta cttccacggg ttgcccggcc aggttgctgc tcagggtctg
    12241 ctcgggttcg atgaccacgc ggatgtccga ggccagtccg ccttcatgca ggctgctgct
    12301 gactatcttg ccgtagcgcg gggtttcttc gccggcgacc acaaagctgg cgcggctgcc
    12361 gatgcgggcc tgggcaaagt tgcggtaagg gaagctcgcc agcaccgtgg cgcgggtgtt
    12421 ttgtggcacc agctcgaaga tcacatcgcc cttgctggcg aactgaccgt cggccaccag
    12481 ctgacgcgcc accttgcagt cgcaggggct ggtcagggtg cctttgatct gtttgccgaa
    12541 cagggtttcg atgttcgctg ggcttagctg ctcgtcgttc aggtggcctt tgagcatctc
    12601 cagcatgctg gcggagaatg aagcaatcgg cgcgcctttg gccacgctgc cgtctgtgcc
    12661 gatcaaactc tgcaccgtgc cttcgcgcgg catggtgacc tgcaggcttg gcacgttgac
    12721 cagggctgac tgggcatgcg tgacgaagta caggccgtat accgacttga agataaaacc
    12781 gaaggccgcc agacccacca ggaagatcgc caggctgaac gtcacggcct tgaggcggcc
    12841 gagtacgctc atggcggtac cgctgttggc gtgcttgcgc gctttggtga agttgtcgcg
    12901 ctgcaaggtg cttagcaggt cgccggcgct gaccagctca ccgcccaggt aggcgctgat
    12961 caggtggcgc agggtggcct gctcgcgcgg cttgaggttg tgcagctggc agccgacgcg
    13021 cttgctgtcg acatccaccg agcgcacctg gaattcgatg tcgataccca ggttgaagct
    13081 gtcgagcttg aattgcagac ggcctttgtg aaagtcaccg atctgcatcg cctgctggtt
    13141 gtcgatcact ccgaaaccac ctgccgagat atcgcagagc tgtaactcga tacgctcgcg
    13201 gctcttgttc aggtagtgca cctgggctgg gatcttcacc cgggcatgct ggcgctgggc
    13261 ttctgactca tgcaccacgt tgacgttgac ggctgtattc atgcttgcaa attcctgttc
    13321 aaaaggggca cggtcgtcac accagcacca tcagggtggc gacgaatagg ctggcggcag
    13381 agaaggtcat ggcccgcgag gaccaggtgt tgaaccagcg ctgaaagctc accaggccgc
    13441 gatcgagctt ggtgttttgg cgggtccagg actgctggtc gagacggaag aacacgtaga
    13501 tcttcaccag cgcgccgacg atctggttgt aatagagaat cagtggatat gccgggccga
    13561 tgtggtgccc ggtgacgctc agcagcaggg tcagaatcag ccgggtcagg ccgatccaca
    13621 gcaggtaaac gaggatgaag cccaagccgt atttgaggct ggcgataatc gccacgctca
    13681 ggccgagcag gctggtccac atcgacacgc gctggtcgaa cagcaccagg ctggtaaacc
    13741 cgcccaggcg gcttacaccc aggcgcaggg cgcgggcgtt ttgccgcagg ttgttgccat
    13801 accagcggaa catcagtttg cggctggcct tgataaagct cttttccggt gggtgctcga
    13861 ctgtgttgat cgccgcatcc ggcacgtaga aggtgtcgta gcccaggcgc atcaggctgt
    13921 accagctgga cttgtcatca ccggtgagga actggaagcg ccccaggcgc cagtgatcaa
    13981 ggtgatcgct ttccacatcg gcgataaacg ccggatcagt caccacttca gcgcggaata
    14041 ccgacatgcg cccggtcatg gtcagcacgc gtttgctcag ggccatcgaa cacatgttca
    14101 ggtggcgctg ggcgaagcgc agcttgtgcc attcgctcat cacataactg ccgcgtacct
    14161 cgcagaactc attggtggtg aggccgccga cattggggaa caacttgaac cagggcacgg
    14221 ttttgcgcac cacgccctcg ccgagtacgg tgtcgccgtc gatcaccgcc actaccgcat
    14281 cggtatccgg catttgccga gagatggcgc gaaagccata ggccaggcca tcccgtttgc
    14341 ccgtgccggg aatgcgcacg aagtccaacg tgacatgttc tggtggctgc atctgcgccc
    14401 agaggctctt caccagcagc tcgtcagaga gttccacgat ggagcagacc accgttgtcg
    14461 ggtagccgca gtcaatcgct tcgcgaatca ctgagcggta cacctgggcc gtggttaggg
    14521 catcgatgcg aaaactggtg accagcagaa acacctggga cgggtcggtg gcgttgccga
    14581 gcttggccac cttgcgccgg tagtgaggga acaccacata gagaaacagc atgccgcgca
    14641 cgaaatgcac ggcacccatg gaataacgcc agatgcctac cgcgccgatc aacaggaggt
    14701 agtcgcgcga ctcggggtcg aacacgctcc tgggcaatgc cagcgcaagc agcatcagca
    14761 gtgataagta gaacagccag ccagcggcct ggttaaggcc gcttttcagc gtgtgtgttt
    14821 gcataaacat atccatcgtt tgtgccagat agccctgggt cggcgcttgg ccgcccaggg
    14881 cttgcttcag ctcacgagct gcagggttta ccagcagatg ccttcgctgc ctgcacgtga
    14941 tgcggtcggc ataaagccca ccagatcgac cacgtgtttg ccgtgcatgg cctgctgcgc
    15001 cagtggggca aagtgcatgt cgctgttgcc cagcacgatg atgtcggcgt tatccaccac
    15061 ggagtcgaag tcactgctga gcagtgagga aacgtggggg atcttcgact cgatgtagtc
    15121 cttgttcgcc ccatagacgc gggcgtactc gacgttgcgg tcgaagatgc tcaggtcgta
    15181 acccttgccg atcagcattt ccgccagctc caccagcggg ctttcacgca ggtcatcggt
    15241 gccggccttg aagctcaaac cgagcagggc gaccttgcgt ttgtcgtagc tggcgatgat
    15301 gtcgaaggcg ttctgcacct ggatggcgtt gctgcgcatc agcgagccaa tcagcggcgc
    15361 gtcgacgtcc atgctgccgg cgcggtagga cagggcgcgt acgtccttgg gcaggcacga
    15421 accgccaaag gcgaagccgg gcttcatgta gtacttggag aggttgagtt tgtgatcctg
    15481 gcagatcacc tccatcacat cgcgtccgtc gacgccggcc gccttggcga tattgccgat
    15541 ctcgttggcg aaggtgacct tggtggcgtg ccagacgttg caggtgtatt tgatcatctc
    15601 ggcgacatcg atgctcttgc ggatgatcgg cgcatccagt tcgctgtaga tgctttccag
    15661 cagatcaccg gaggcctgat ccagctcacc gatcaccgtc atgggcggga agtcgtaatc
    15721 cttgatcgcg gtgctctcgc gcaggaactc ggggtttacc gccaggccga agtcgacgcc
    15781 ggcctgtttg cccgagcagt cttcgaggat cggcaacacc acgtttttgg ccgtgcccgg
    15841 cagtaccgtg ctgcgtacca ccacggtatg ccgttcggtt ttgtcgcgca tggccaggcc
    15901 gatttcgcgg cacacgccct cgatgtaatt gagctccaga tcgccgttct tcttgctcgg
    15961 tgtacccacg cagataaacg agacgtcgct gttaagcacc gcatcgatca cgtcggtggt
    16021 gccgcgcaaa tttcccgaag ccagcccctg ttgcagcagt tcttccaggc cgggttcgac
    16081 gattggcgat ttgccattgt taatcagatc aatcttgctg gcggaaacat ccacgccgat
    16141 cacttgatga ccgcgtgccg acaaacaacc agcacaaaca gccccgacat aacctaaacc
    16201 gaagatgcta attcgcatcg cattcacctc gtccatatgt tgcactatta atttcgtggc
    16261 aattagccac agttattcgt tagttctgca cgcacaggtg cgtgaactaa tttaggcata
    16321 gagagttggt tggtaacgtg tagcaaccgg agtcgggctt tatatgtagc acgcccgttc
    16381 tgctttctgg ccagtggcgc gggagcgctc tggcttgagg cttataagtg attcattgag
    16441 ctgtcgatag ttccacctat atttgccagt aactttcttt agtgagcgcc agcgccggat
    16501 aaggggccgg gactagtggc agatatattc atgcggaagt tgcatgaaaa aatcatcatg
    16561 tcgctatgag tccgatgttt ttctgatggt tcctaccgtt cgtcatcttt tgaaatgaaa
    16621 tgagaactgt ttggtagttg cactttgata gcactttgca attgcgcagt tgagtaactt
    16681 gcgcggtgtg tcattgtgct ggaaagttca ataagaaagc gtcggcttaa taaagctgac
    16741 tgtttgcata ggtattgaag tgtgcggcga agagccagga acgggccgtt ggcgcggccc
    16801 gcaggggagg aagggggtta gtcctgggga tggtcgcgga ggaaaaccag ggtgtcggtt
    16861 ttcgagtttt cggccgagta gcggtagcct tcgtaggtga aatccttgag gccggtcgga
    16921 tcgctcaggc gctccttgat cacgtagcgt gccatcaggc cgcgggcttt ttttgcgtag
    16981 aagctgatga tcttgtactg gccattcttc agatccttga attcggtgtc gatgatccgc
    17041 gcattcagcg ccttgcgttt gaccgcactg aagtattcgt tggacgccag attgagcaac
    17101 acctcatcgc cttgggccgc cagcgcctca ttaaaccagc cgctgatgcg ctcaccccag
    17161 aagtcataaa ggttattgcc gcgcgggttg gccagtttgg tacccatttc caggcgatag
    17221 ggctgcatca ggtcgagcgg gcgtagcacg ccgtacaggc cggaaagcat gcgcaggtgg
    17281 gtttgcgcga agtcgaagtc ggcctcgctg aaatcctcgg cgtttagccc ggtgtacacg
    17341 tcgcccttga acgccagcag cgcctgcttg gcattggacg gattgaacgg ccgctcccag
    17401 ctgccgaagc gtgcggcatt caggccagcg agtttgtccg agaggtgcat cagctcggcg
    17461 atctgcgccg gggtgaagtc gcgcagctgc tggatcaggt cctgggcgtg gtcgaggtgt
    17521 tcgggctggg taaaacgcgg ggtcgcaggt ggcgtctcgt aatcgagggt cttggccggg
    17581 gaaatcacca tcaacataag ctgtgctcct aaaagtctgc ggcgatttta ggccgagcgg
    17641 tggtcgcggc tctagctatt atgccgatgg attattttgt ggcctgttgg tgagcccctt
    17701 gctgggcgta ggagcgggcc atgcccgcga tgcagtttgc aggcagccgc tttcgcggcc
    17761 atgggctagg cgccccgccg ctcctacgga tcgtccgggt aacatgcttg acccaggggc
    17821 cgccacgggg ttttagtctg cccaggtcga actctcaagg agctgtcatg accgcttgca
    17881 accattcgcg ctgggagcca tcccacgatt accgcccgct ggaaactact gccgaacgtc
    17941 aggcctggcg cgttgccgcg ctgaccgggg tgaccatgct ggtggaaatt gtcgccggct
    18001 atgcgttcaa ctccatggcc ttgctggccg atggctggca catggcctca catatgctcg
    18061 ctatcggcct gacggctctg gcgtacctgt tggcgcggcg ctacgcccac gacccacgtt
    18121 ttgcctttgg cacctggaag gttgaggtgc tggcgggttt tgccagttca ctgatgttgc
    18181 tggtggtagt ggcgatgctg gcgttcgagt ccttctggcg tttgttgcag cctcaggtaa
    18241 tcgccttcga gatggccctg tgggtggcgg tgatcggctt gctggtcaac ctggcttccg
    18301 cctggctgct gcgcgatagc catgatcacg gacatgctca tagccacgcc ggtgttcatg
    18361 ctcatgccga tcacgatcac gatcacgatc acgatcacga tcacgatcac ccagcgccag
    18421 cggcgggtaa ggaccttaat cgccatgctg cctttatcca cgtgctggcc gatgcgctga
    18481 cttcggtggc ggctatcgtc gccttgcttg gcggcctgct gctgggctgg gattggctcg
    18541 atccgctgat gggcgtcatc ggtgcggtaa ttatcgcgat atgggccaag ggcctgttga
    18601 tcgagaccgg caaggtactg ctcgatcgcg agatggacag cccggtggtc gcgcgcgtac
    18661 gcagcgcctt ggcccaggag gccgataccg aactggcgga cctgcaccta tggcgcgtgg
    18721 gtcgggcgca gttcgcctgc atcgtcagcg tggtcaccca tggcgatagt tcggcggatc
    18781 gatacaaggc gcggctggcg gggattgcgg agttggtgca tgtgaccgtg gaggtcaatc
    18841 gctgtgccga taagcacggg gtggagcggg gctgacaggt aaaaatatgt cggctcgaac
    18901 gccggtggga cttccgactt taacggcgct tgcctggtgc gccaagcgct atttgtcgct
    18961 ccgacaaata aacctgaaaa acccaaaaaa tacgtttttc tgtcatcttt tttggagtat
    19021 ctaaaaggca agaccgccga accctgcaga caggtggcgg ccattggccc tcaccttttt
    19081 gcagccttct cagccccggc actgaggagt cgacggcccc tccgcggcgc agactcgccc
    19141 ctgcgttgct tggctactac aagaaggtga ccgagtatgg atgaccacgg acgctccaag
    19201 cccaccgccc caaccctgta cgccctcgat accaatgtgc tgatccacga ccccaacgcg
    19261 ctgctgaatt ttcaggagca ccacgttgcc attcccatga ccgtactgga ggaactggac
    19321 aagcttaaaa ccggcaagca gggcgtggcc gccgagtgcc gccaggccat ccgccttatc
    19381 gacaagattc tcggcagcgc caacccggag gaagtggaac acggcgtcgc catccagcgt
    19441 gacaagagcg gcccgtgcgg ctttctctca attcttatga gcaaaagcgc caccccggtc
    19501 acctggttgc cggaagatct caacgacaac aagatcatca atcagctggt cgagctgaag
    19561 tcccgtcgcc ccggtctcaa cgtggttctg gtgaccaaag acatcaacat gctcctgaag
    19621 gcgcgcgcct gtggcatcga ttcggaggat taccacaccg accaactggt cgacgacgtg
    19681 tcgctgctgt cccagggcta ccacaacatg gacggctcct tctgggaccg tgtgaacaag
    19741 gtggaaaccc gccaggatca cggccgtacc tggcaccgcg tgcaactcac cgacaacctg
    19801 ccggcggtgc acgtcaacga gttcatcatc gacgagcagg gttttgtcgg ctggatcaag
    19861 ggcatcaagc acggcgaact gctgattctc gacatgcacc aggaaccctt gatgcatcag
    19921 gaagcctggg gtctgcggcc acgggatatc taccagtcac tggcgttgtt tgccttactt
    19981 gatccggata ttcacctggt caacctgtcc ggcgcggccg gttccggtaa aaccatcctg
    20041 gccctggcgg cggctatcga acagaccatg gtcagcaagc gctatcggcg catcatcgcc
    20101 acacgcagcg tgcaggggct ggatcaggag attggctttc tgcctggcac cgaggcggag
    20161 aagatggagc cctggctcgg cgccatcacc gacaacctcg aagccctgca tatggacgac
    20221 gagaacaccc acggcagcgt cgactacatc ctgcaaaagg tcccgctgca gttcaaatcc
    20281 ctcaactaca tccgtggccg tagcttccaa cagagtctga tcctgatcga cgaatgccag
    20341 aacctcaccc cgcaccagat gaaaaccatc atcacccgtg caggcagcgg ttccaaagtg
    20401 gtgtgcctgg gtaacctggc gcagatcgat acgccttacc tgtcggcgcc cagctcgggc
    20461 ctgacctacc tgacggagcg cttcaaagac ttcgagcatg gcgtgcatat caccctgcaa
    20521 ggggtgccgc gctcgatcct cgccgagtac gcc
//
LOCUS       DASRUM010000314        29978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2336288, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000314 DASRUM010000000
VERSION     DASRUM010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 29978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29978
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2336288"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /gene="hmpA"
                     /locus_tag="VFF22_21045"
     CDS             complement(<1..859)
                     /gene="hmpA"
                     /locus_tag="VFF22_21045"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017362532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HZX19341.1"
                     /translation="MLTPEQTALIKATVPLLESGGEALTRHFYQMMLSEYPEVRALFN
                     QAHQASGGQQRALANAVLMYARHIDRLEALGPLVGQIVNKHVSLQILPEHYPIVGSCL
                     LRAIREVLGEEIATDAVIEAWAVAYGQLADILIGAEEAAYSANANAEGGWRGARSFRL
                     SRKVQESEEIVSLYLAPADGGALVAFQPGQYIGLRLLLDGDEQRRNYSLSALSNGREY
                     RISVKREVGGKVSNYLHDQLQVGDSLELFAPAGNFVLRESAKPLVLITAGVGITPALS
                     MLEAARDTCR"
     gene            1036..2589
                     /gene="norR"
                     /locus_tag="VFF22_21050"
     CDS             1036..2589
                     /gene="norR"
                     /locus_tag="VFF22_21050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HZX19342.1"
                     /translation="MTANPLLTALIPLVADLSRELPEAERYRRLLQALRQLLPCDAVA
                     LLKLDGDVLIPLAVEGLSADTLGRRFKLSEQPRLQALLAQRGPTRFATDCEMPDPYDG
                     LVDGHRGHLPVHDCLGCPLYVQDQPWGLLTLDSLDPASFGRVDLDNLQAFASLAAATV
                     MASERINALLQRAEDQHQLAEAYKQAAGQQRPRELIGQSAVHKRLQAEIALVGNSELS
                     VLISGETGVGKELVAEALHAASPRAAKPLISLNCAALPDNLVESELFGHVRGAFTGAH
                     NERRGKFELADGGSLFLDEIGELPLPAQAKLLRVLQSGQLQRVGADQEQRVNVRVLAA
                     SNRDLAEEVRAGRFRADLYHRLSVYPLKVPALRERGRDVLLLTGYFLEENRARLGLRS
                     LRLTADAQASLLAYNWPGNVRELEHLIGRAALKALAGCSERPRILSIDSAHLDLPTSQ
                     PVTAPATQRAAEPHSELPLREALDAYQKQLISQALARHQNKWAAAARELGIDRANLIR
                     LAKRLGLQN"
     gene            2772..4007
                     /locus_tag="VFF22_21055"
     CDS             2772..4007
                     /locus_tag="VFF22_21055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007158593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HZX19343.1"
                     /translation="MADEAKPDRIKVDAAQLTIGMYVVELDRPWTDTTFLFQGFRIRQ
                     QQDIRLLQEVCSYVWVDARQSVTVHDQVTANIAQSEALQPVIAKVDFNLEMLQAAPTW
                     NAAREESLRILQAVKLGQELDVEAVKAVIKDCVESILRNPAAMLWLARIKNSDEYTAE
                     HSLRVSILSIALAKELGLPVYQLEQIGVCGMLHDVGKIKVPNEILNKPGALTVEELCI
                     MQSHAAEGRKLLMSNQQVTAATVDVAYSHHERLDGQGYPRGLNASKIPYFAKIIAVAD
                     SYDAINSDRVYSKGKSSLEALRILLEAVNSHFDEEIVGCFIRMVGIYPPGEIVELTNG
                     EVAIIIGCPPGNKLKPRVLRVLDGQKQPCKESIIDLGKTANDPSGKPYRIHEVHSSGA
                     FGIDIDAFRSKGLVIPTHL"
     gene            complement(4065..4141)
                     /locus_tag="VFF22_21060"
     tRNA            complement(4065..4141)
                     /locus_tag="VFF22_21060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4105..4107),aa:Arg,seq:ccg)
     gene            4490..5938
                     /gene="gabD"
                     /locus_tag="VFF22_21065"
     CDS             4490..5938
                     /gene="gabD"
                     /locus_tag="VFF22_21065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZX19344.1"
                     /translation="MQLKDTQLFRQQAYIDGTWLDADNGQTINVNNPATGEIIGIVPK
                     MGAAETRRAIEAADRALPAWRALTAKERSNKLRRWFELLIENQDDLGRLMTLEQGKPL
                     AEAKGEIVYAASFIEWFAEEAKRVYGDVIPGHQPDKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVIKPASQTPFSALALVALAERAGIPKGVLSVVTGSAGEVGG
                     ELTSNPIVRKLSFTGSTEIGRQLMAECAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALISKYRNNGQTCVCANRLYIQDGVYDAFAEKLAAAVAKLQIGNGLDEGTTTGPLIDD
                     KAVAKVQEHIADALSKGARLLAGGKPHALGGSFFEPTILVDVPKNAAVAKEETFGPLA
                     PLFRFKDEAEVIAMSNDTEFGLASYFYARDLGRVFRVAEALEYGMVGVNTGLISNEVA
                     PFGGMKASGLGREGSKYGIEDYLEIKYLCLGI"
     gene            6116..7396
                     /gene="gabT"
                     /locus_tag="VFF22_21070"
     CDS             6116..7396
                     /gene="gabT"
                     /locus_tag="VFF22_21070"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HZX19345.1"
                     /translation="MSKTNESLMQRRTAAVPRGVGQIHPIFAESAKNATVTDVEGREF
                     IDFAGGIAVLNTGHLHPKVIAAVQEQLTKLTHTCFQVLAYEPYVELCEKVNAKVPGDF
                     AKKTLLVTTGSEAVENAVKIARAATGRAGVIAFTGGYHGRTMMTLGLTGKVVPYSAGM
                     GLMPGGIFRAQYPCALHGVSTDDAIASIERIFKNDAEPRDIAAIIIEPVQGEGGFYVA
                     PKDFMLRLRALCDQHGILLIADEVQTGAGRTGTFFAMEQMGVAADLTTFAKSIAGGFP
                     VAGVCGKAEYMDAIAPGGLGGTYAGSPIACAAALAVMEVFEEEQLLDRCKAVGERLVT
                     GLKAIQAKHNSIGEVRALGAMIAMELCEGGDVHKPNAALTGQIVAKAREKGLILLSCG
                     TYGNVLRVLVPLTAEDALLDRGLAIIAECFDELA"
     gene            complement(7506..8882)
                     /locus_tag="VFF22_21075"
     CDS             complement(7506..8882)
                     /locus_tag="VFF22_21075"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZX19346.1"
                     /translation="MVDLSERRALEERLQYLAAHDSLTGLLNRRELERLLRESLKGQD
                     TPSYSHLLCLDLDQFKQVNDLCGHSAGDQLLRQLSGHLLHQLPRHAELARIGGDEFAV
                     LLREVDSAAALEHAESLRHCVEQFVFSWQGRPFRVQASIGLLELDSGVSDWETALNWA
                     DSASQLAKHLGRNRVQQFNPADGVLLEHQRQLQWITRLREAIELGHFELFYQPVMALQ
                     NASDGLRYEVLLRYHDPISGEWVSPGQFLGAAERYGFLGAIDRWVVMRLLQWLADNPR
                     HVQQLAQVNFNLTASSLLNAHFHEMLHSELLRHNLPAHKLCIEVTEMVALGELGVSSQ
                     WIKQLRSLGLKVALDDFGSGFASYAYLRHLPLDIVKIDGSFISGLESDPINQAMVGSM
                     QQIASQLGLSTVAEFVESQATLDCVRGLGLDYAQGYFIGHPQPLTQLADSQLEELQMK
                     LVLDTSGR"
     gene            complement(8889..10406)
                     /locus_tag="VFF22_21080"
     CDS             complement(8889..10406)
                     /locus_tag="VFF22_21080"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HZX19347.1"
                     /translation="MLHLFNLPLLAALLCFALGSVSAHAETLPLLVLSSEQLKQPVEV
                     PASLVLEDPSGNLTAAEVQQRIASEGKRIDGVPRLGYSTSAWWLAFRLDAPVAERLDL
                     MIDRPFLDDIEVWLYDRERLIAPFYSGDHRDFLLRGAPSPHFLLNLPRLGEGPHTLLL
                     RVQSGSAVSVPMQLVAAEQSPLLLAEHWMRSGLLLGALLSLTLFFLVKFSALRETQLV
                     WFCLTVASVALYNASLHGVTSLLAPQWVWLSTQMANLANPGMLIFSSLFLADSLKLEL
                     GRVRHLRNALFAVTLLVCGWTIVSYGHFRSYQTLNVLILLNGAFQLLLLILGVRQRRP
                     YAIGYLLCWSSALLLMLLVPLGRSGVIPLPPSFNYMHAYLPVLSMLLFGGILDKQLEQ
                     IRRALLHSKAQALGNLEQYQALFSSSGEGIFRCTRSGALIEANPSFAALLGLAQDSSE
                     LSMQQLLGVEQWAEQVAQLHTEQPAISQECQIHGLDGQSHWVYLSLHLRPTQDCI"
     gene            complement(10491..10925)
                     /locus_tag="VFF22_21085"
     CDS             complement(10491..10925)
                     /locus_tag="VFF22_21085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HZX19348.1"
                     /translation="MLVRLTYASRASHGVSSELIREILDSSQRNNPGKGLTGILCCNA
                     DIFLQALEGPRAEVNALYNRLADDNRHKDLTILDYAEISMRRYSNWSMGWAGAKQANR
                     ELFLKYSASDRFDPFSMSAEQITGLLLELGETVSAIKSPVLS"
     gene            11126..12091
                     /gene="arsS"
                     /locus_tag="VFF22_21090"
     CDS             11126..12091
                     /gene="arsS"
                     /locus_tag="VFF22_21090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339360.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HZX19349.1"
                     /translation="MLPLLDQLAFPAIRRGQLEALQINLTYRCNQRCLHCHVNAGPTR
                     TEAVTDESLTLLHQVIDAHPVHTLDLTGGAPEMHPRFREIVTHARREGLRVIDRCNLT
                     ILSEPGYEDLAQFLADQGVEVSASLPCYSRDNVDKQRGDGVFDASIKGLQQLNALGYG
                     QPDSGLILNLVYNPQGPSLPPPQAALEADYKRHLAEDFGIVFNHLLTITNQPIARFGS
                     TLVSKGQFNGYMQLLRDSYRPENLEPLMCRSLVSVDWQGYLYDCDFNQMLDLPLQLPE
                     VIGRDRPHLRDLLDATLAGNHIVTRDHCYACTAGQGSSCGGALND"
     gene            12191..13570
                     /locus_tag="VFF22_21095"
     CDS             12191..13570
                     /locus_tag="VFF22_21095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HZX19350.1"
                     /translation="MQPTSLTGLFFWGFLVVYGALMLALSPRAVTLGGFFNGEDNQGR
                     SASPWLITSSIFISWIFAKSVTNAANLGATYGLVGGLAYAMYWLSIPLTGFVIYRLRR
                     RFAATGLVNFLSSHYGRGAALAFSAAILIRLFNEVWSNTAVVGGYYGDSGSIEFIASA
                     LLFTAVTLAYSLRGGLRSSILTDAAQAAIFVIALVWVLGLVLPQHSPTELASTSHWAL
                     NAGVDLLLVAGLQAFSYGFHDPVLTDRGFISEEKAMRRSFLIAGILGFIAILAFSLIG
                     VHAQLVGIAASDNVPAALAKSLGIGALLVMTVVMVSAAGSTLDSTFSSLAKLAGRELP
                     RLAGRDLGQKAIGVGMAVMVVFALLGNLPMIAGTDILKATTISGTMVIGLAPVFVLHG
                     LTTPTRLGFHLSFWTGLGLGVALTLNWIPQSWAIGDGKYALLLGTNLYGLGLCVLGYL
                     LPSWLRRTA"
     gene            13668..14636
                     /locus_tag="VFF22_21100"
     CDS             13668..14636
                     /locus_tag="VFF22_21100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX19351.1"
                     /translation="MRAGRDCPLDYRLARDAFSGEPLFDCDMLYVVGGLYGNQQALAA
                     LEKRLAAEAGARLVFNGDAHWFDRDPEIFQTIEQGLNGHLALRGNVETELGRADDSGA
                     GCGCAYPLTVEEAVVERSNAIQLELNKTVQGLPGIAERLATRPSTAIVSVAGQRVAIS
                     HGDEQSLAGWGCSRESLRESERQQQLDTWFSTNNVQVLATSHTCSAVALNLEHGALIN
                     NGAAGLPNFAGGRYGLLSRIATTPHPAALYRCQRDGLFIEALPLNYDHTAFLADFNRQ
                     WPADSPAALSYRSRVLGQLTDRPETALLADFSLCQSLCALEALTDE"
     gene            14629..16770
                     /locus_tag="VFF22_21105"
     CDS             14629..16770
                     /locus_tag="VFF22_21105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZX19352.1"
                     /translation="MNSRKLALMGLILALLGSFFVFDIGQYLNLEALKAQQAALNAQV
                     AAQPWLAAGLFFLAYVAVTALSLPGAALMTLLAGALFGLLEGFVLVSFASTLGATLAM
                     LSSRFLLRDWVQSRFGQRLAGIDAGIEREGAFYLFALRLVPLFPFFLINLAMGLTRLP
                     VRTYWWVSQLGMLPGTLVYVNAGRELGQLDSLAGILSPGLLGAFVLLGVFPLIARKLL
                     GLVQARRVYAGWHKPKRFERNLLVIGAGAGGLVSAYIAAAVKAKVSLIEKHQMGGDCL
                     NTGCVPSKALLRSAKLANELKKGQALGFSGVQGTVDFPAVMQRIQRVIADIEPHDSVE
                     RYTSLGVEVIQGEAKILSPWSVEVNGQTLTSRNLIIAAGARPLVPKIPGIEQVQGYTS
                     DSIWSLREQPRWLLVLGGGPIGCELAQAFQRLGSQVIQVELAERLLPREDADASELVL
                     SSLRADGVDVRLQHRAERFEVVDGEQRMVARRLDTGEEVTIAFDGLLLALGRVANVTG
                     YGVEELGLTVRPNGTLETDEYLATRFPNIYAVGDVTGPYQFTHVAAHQAWYAAVNALF
                     SGFKRYKVDYRVIPACTFTDPEVARVGLNEGEAKAQNIAYEVTRYGIDDLDRAIADEA
                     AHGFVKVLTVPGKDQILGVTIVGEHAGELLAEYVLAMKHGLGLNKILGTIHTYPTMAE
                     ANKYAAGEWKRTHAPQGLLRLVERFHNWRRS"
     gene            16770..17483
                     /locus_tag="VFF22_21110"
     CDS             16770..17483
                     /locus_tag="VFF22_21110"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HZX19353.1"
                     /translation="MRGLLLALLVLTPLAQANNQLTPWTAGPPNDEWSPPWRLADIPK
                     LKPSERSLVEQDRPQVLRIKAEAAAGGLLHPLDLPGDQPWQLSWQWRTEQKLEQARFA
                     TREGDDYAARVYVLLDYPLDSLSFVTRQKLRLARTFFDPDLPAATLSYVWDNRAAPGI
                     HARSAYTETVEMMVLRGPEAPLGQWLSESRNLNADAIAAFGSAPRKILGVVFATDSDN
                     TGESAESYFAAPQLQLSNP"
     gene            complement(17567..18637)
                     /locus_tag="VFF22_21115"
     CDS             complement(17567..18637)
                     /locus_tag="VFF22_21115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017676051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HZX19354.1"
                     /translation="MPHSPASTAALNDAQRAALIRQQVMAHGVELRQRFPILNHQDAL
                     GAGILTFALAGMIGSAALYIGGYLAWWACLLLNAFFASLTHELEHDLIHSMYFRKRRV
                     PHNLMMALVWLARPSTINPWVRRHLHLNHHKVSGSEQDMEERAITNGEPWGFARLLMV
                     GDNMMSSFIRWLRASNPKHRRLILTRTLKVYAPLGLLNWGTWYVFLGFHLIDFAATAL
                     GAPVAWSATTLSVMAVVNIAVVVLVGPNVLRTFCLHFVSSNMHYYGDVEAGNVIQQTQ
                     VLNPWWLWPLQAFCFNFGSSHAIHHFVVKEPFYIRQLSVPFAHKVMREQGVRFNDFGT
                     FARANRWTRREQAEPAKQAQVA"
     gene            18737..19750
                     /locus_tag="VFF22_21120"
     CDS             18737..19750
                     /locus_tag="VFF22_21120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZX19355.1"
                     /translation="MTAEPTTLASWTRALRKQLDALGLDSAALCLKAGLEPSLLDDPN
                     ARCPLSITTRLWQLAVEASGDPALGLKTSQFVSPTTFHALGYALGASSSLREMFERIV
                     RYHRVVSDALELELREAGDCYEFCFRVPPGSPPPAPEALDAFAAIYVRSCRNRLGRDF
                     APLAVQLQRAEPADAQPWHAVFRAPLLFAAPENLLRFPRAAFEQRLDDGNPELAEHNE
                     TVLKRTLEQLHHATWTHKVRACLEAQLPDGEPSAERIAQTLHLSLRSLQRHLADEGGN
                     YEGLLADTRHSLALQHMHDPRCSISEIAYLLGFADTSSFSRAFKRWTGQAPSHYRDGL
                     KQP"
     gene            19747..20817
                     /locus_tag="VFF22_21125"
     CDS             19747..20817
                     /locus_tag="VFF22_21125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZX19356.1"
                     /translation="MKQYDLLLIGAGHAHLGVLRRWALVERPPGRIALLSAGPVAWYS
                     GMLPGLLAGRHRPEDCQVELQALCRAAKVELIISPVESLSAAQQQVQLADGQTLSASW
                     LSLNVGAQVSAPAQQGDAMQLLAVKPFAEFLEGWQRWQAEPQPLAILGGGAAGVELAL
                     ALAGKVPQLALFCAGHLLDGLSAGVRLRALGHLRQRGVWVREDCPISRIADDCLLSGD
                     EPVWRGQRLLLASGAKAFAWPAHSGLGCDEQGFVLIAPTLQSYTHPTIFAVGDCASLP
                     GARKSGVYSVRQGPVLAANLNAALRGLALKRYRPQRQSLALLATGDGGALLDWHGHSA
                     GGRFYGRWKDFLDKGFIQRHRL"
     gene            21077..22645
                     /locus_tag="VFF22_21130"
     CDS             21077..22645
                     /locus_tag="VFF22_21130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZX19357.1"
                     /translation="MRSAFITYPKACLLHAIGLAGLMLTYQQIELPWFVSIPSFLLLA
                     LWPWLGPWQRQHEEQPAHAKPHDTFSDLSQSLSRQTCHNALAAAEVAHAAQQLAGKLQ
                     SQLSATEQINQAAEAITHTEQDNAQRAEQTLLAAQSVRQNSSSGQQELRQAIETMQHL
                     SAQTATSRELIDGLSARTEQIEKVTQVIQSIASQTNLLALNAAIEAARAGELGRGFAV
                     VADEVRNLAGRTSSATEEVGQIVSDIRQQSLAVVSHMQQQAEQLTLAAQQVEHTGEQL
                     HGIAELAVDVESQVSQIDSGTRQNHERLAELSVAVGQLRGDVSDSEGQTRQLSSAAER
                     LVAQAESVSEQLAEVGLDEYHQRVYDLARSAAALIAAQFEADLHSGRISANDLFDRHY
                     QPIAGSFPAKYKTRFDGYADQVLPAIQEPLLSQHEGLVFAIACTAEGYVPTHNNAFNH
                     AATGDRAVDTLKSRSKRLFNDRTGIRCGSHQKPLLLQTYMRDTGELMHDLSVPIMVNG
                     RHWGGLRLGYKPEP"
     gene            22796..23479
                     /locus_tag="VFF22_21135"
     CDS             22796..23479
                     /locus_tag="VFF22_21135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019837860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HZX19358.1"
                     /translation="MKILLVLTSHNQLGDTGHKTGFWLEEFASPYYVFKDAGAQLTLA
                     SPKGGQPPLDPKSDVADAQTPTTERFKADVAAQQALANTLPLSQVQADDFDAVFYPGG
                     HGPLWDLAEDRTSIALIERFQAQNKPVAAVCHAPGVLRHVKAANGLPLVSGKKVTGFS
                     NSEEAAVQLTEVVPFLVQDMLITNGGHYSSANDWQSHVLVDGLLITGQNPSSSDATAE
                     ALIKLLKSR"
     gene            complement(23559..24788)
                     /locus_tag="VFF22_21140"
     CDS             complement(23559..24788)
                     /locus_tag="VFF22_21140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015270225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HZX19359.1"
                     /translation="MTAFQPLQLPNGTTVFNRIAKAAMEENLADASQGPSAELLQLYQ
                     AWAEGGAGLLLTGNVMVDRRAMTGPGGVVLEDERQLEKFREWARIGRAQGAQFWMQIN
                     HPGRQMQANLGQQTVAPSAVALELGGLSKMFPVPKALDEAEIHELIQRFARTAQLAEQ
                     AGFSGVQIHAAHGYLLSQFLSPISNKRTDSWGGSLENRARLLLEVVKAVRALVAPSFC
                     VAVKLNSADFQRGGFDAADAKRVVQLLNQLDVDLVELSGGSYEAPAMQGDARDGRTLA
                     REAYFLEFAGEIAAAATMPVMVTGGIRRLPVVEQVLASGVAMAGIATALAINPALPKQ
                     WQAGQSSSAELAPIRWKHKALASLAYMAVVKFQLRQLSLGKHTHAGVSPLRALLLEQL
                     KTARRTRQYKRLMAAGS"
     gene            24866..25270
                     /locus_tag="VFF22_21145"
     CDS             24866..25270
                     /locus_tag="VFF22_21145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008011039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZX19360.1"
                     /translation="MKIGELATRSGLAASRIRFYEASGLINAQRQANGYRSYTEQTLQ
                     TLGIISCAQQAGFSLEEIRRLLPDANPQGWAHEQMLGSLQRKVVEIEAMQQRLAQNKA
                     QLLTIIATIESKPEGMPCTENAERVLANLRGS"
     gene            25622..26935
                     /locus_tag="VFF22_21150"
     CDS             25622..26935
                     /locus_tag="VFF22_21150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZX19361.1"
                     /translation="MNAAIKPVTPAAERCPSYYSASLNEETDYPTLQGEVSVDVVIIG
                     GGFTGVASAVELAERGLKVAIVEANKIGWGATGRNGGQVTGSLSGDAAMNKQMRKTLG
                     EEVEDFIWHLRWRGHEIIKQRVEKYGIQCDLKHGHLHAAMKPVHMQELQATYAEAQRR
                     GMGADVTLLDRAGVQSHLASDLYSGALKNSRNMHLHPLNLCIGEAKAAASLGALIFEH
                     SEVLEIIHGDKPAVVTAAGRINAKQVLLAGDVYHKLEPKKLKGMIFPAMGGIVTTAPL
                     GELAKEINPQDLAVYDCRFVLDYYRMTADGRLLFGGGCNYSGRDSRDIAAELRPGIEQ
                     TFPQLKGVAIDFQWSCAMGIVINRIPQLGKLSDNVWYCQGYSGHGIATTHIMGEIMAN
                     ALTGTLGHYDTFAACSQIKVPLGDLFGNPMLAAGMWYYQMLEKLR"
     gene            complement(27033..29015)
                     /locus_tag="VFF22_21155"
     CDS             complement(27033..29015)
                     /locus_tag="VFF22_21155"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HZX19362.1"
                     /translation="MPIPAPVTHADLPLVLAGPLLRRMEPQRLVLWLVGSQPLSFSLQ
                     LSYGDAPEPQSYPLQGDSCQVIQIGEQAFIHLIDLALDAALPCDVQIDYDLLIEQPGQ
                     AALGIADWAPHLLYDGATQANFVLRARLDQLLHGSCRKPHHPAADGLLCADRLLAEPH
                     DPEQRPGLLLMSGDQIYADDVSGPLLRAIHQVIERLGLFGEHLEGAVVDDSTALYQHP
                     ASYYQRAELLPALKSNETLRERFFGGVEKPVFTTSSADNHLVTCAEVLAMYLLVWSPV
                     PWTLLDAEMPALSAEEVPRYQAERGHIEAFRGGLGQVARALAHLPTLMIFDDHDITDD
                     WNLSAQWEETAYGHPFSKRIIGNALIAYLLCQGWGNNPDVFAKPLQMIRAMTEGRDAA
                     NLMNSQPQDALIDELLSFHQWQFVLPSSPALVVLDTRTRRWRSERNLKRPSGLLDWEA
                     LCEFQQELLDHPAAIIVSPAPMFGVKLIETIQKFFSWAGHPLLVDAENWMAHRGAAQV
                     MLNIFRHSRTPGNYVILSGDVHYSFVYEVLIRQRKRGPKLWQITSSGLKNEFPPRLLD
                     WFDRLNRWLYAPWSPLNWLTTRRRMQVSPRIPSRSKAGERLWNGAGLGQVFFNEQGQP
                     SAIYQLNADGSPAVAFVDDRDEAPAPQVAHSLVGKG"
     gene            complement(29109..29792)
                     /locus_tag="VFF22_21160"
     CDS             complement(29109..29792)
                     /locus_tag="VFF22_21160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZX19363.1"
                     /translation="MNSVRSYQARLCQGHWFSALPAALQEALLATAQVQQLAPGQVLF
                     RRGDKPCGLYAVVEGGMRIGAVSAQGKEALLTLVEPPYWFGEISLFDGQPRTHDAFAE
                     GATTLLLVPQARLLTLLEQQPQYWRDFALLMSHKLRLAFIALEEMSLLPAAPRLARRL
                     LLIAENYGEGEPRRVIHLPQEQLALMLAISRQTTNQILKELEGQGIVRLTYGEIEILD
                     LPRLRQASQ"
     gene            29885..>29978
                     /locus_tag="VFF22_21165"
     CDS             29885..>29978
                     /locus_tag="VFF22_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HZX19364.1"
                     /translation="MKTLVDHLAQYAAYHRDQRNILSHFIGIPLI"
BASE COUNT         5795 a         9685 c         9180 g         5318 t
ORIGIN      
        1 cgcggcacgt gtcgcgcgcc gcttccaaca tgctcagcgc aggggtgatg ccgacacccg
       61 cagtgatcaa caccagcggc ttggccgact cgcgcaggac gaaatttccc gctggggcaa
      121 acagctccag gctgtcgccg acctgcagct ggtcatgcag gtaattggag accttgccgc
      181 cgacctcgcg cttgacgctg atgcggtact cacggccatt gctcagggcc gacagcgagt
      241 agttgcggcg ctgctcgtca ccgtccagca gcagacgcag gccgatgtat tgacccggct
      301 ggaacgccac cagcgcaccg ccatcggctg gagccaggta cagcgaaacg atctcctcgc
      361 tctcctgcac cttgcggctc agacgaaagc tgcgcgctcc gcgccagcca ccctcggcat
      421 tggcgttggc gctataagca gcttcttcgg cgccaatgag gatgtccgcc agttgcccat
      481 acgccacggc ccaggcttcg atcaccgcat cggtggcgat ctcctcgccc agcacttcac
      541 ggatcgcccg cagcaggcaa ctgccgacaa tcgggtaatg ctcgggcagg atctgcagcg
      601 acacatgctt gttgacgatc tgcccgacta acggccccag cgcctccagg cggtcgatat
      661 gccgggcgta catcagcacc gcatttgcca gggcgcgctg ttggccgccg ctggcctgat
      721 gcgcctggtt gaacagcgcg cgcacctcgg ggtactcgct cagcatcatc tggtagaagt
      781 gccgggtcag ggcttcgccg ccgctttcca gcagcggtac ggtggctttg atcagggcgg
      841 tttgttcggg ggttagcatg caaggcttcc tcgtcaagtc gacacgctgt acttgcgcag
      901 cggtctggtt catatcaccc tgactgttac aggaagcgtg ccaattattt tattcatatt
      961 aatcatagac ttataaataa acgtgtcttt atgacagcgt gcatttcgag tcttaatgac
     1021 atctatcgtg tccttatgac agcaaaccca ctgcttaccg cactaattcc cctggtcgct
     1081 gatctctccc gcgagctgcc ggaagccgag cgctatcgcc gcctgctgca agcgctgcgg
     1141 cagctgctgc catgcgacgc cgtggcgctg ctcaaactcg acggcgatgt gctgattccg
     1201 ctggccgttg aaggactgag cgccgacacc ctcggccggc gcttcaaact cagcgaacag
     1261 ccgcgtctac aggcgctgct ggcgcaacgc gggccgaccc gttttgccac cgattgcgag
     1321 atgcccgacc cctatgacgg cctggtcgac ggtcatcgcg gtcacctgcc cgtgcacgac
     1381 tgcctcggct gcccgctgta tgtgcaggac cagccctggg gcctgctgac cctcgattcg
     1441 cttgatccgg cgagcttcgg ccgggtcgat ctggataacc tgcaggcctt cgccagcctg
     1501 gctgccgcca ccgtgatggc cagcgagcgg atcaacgcgc tgctgcaacg tgccgaggat
     1561 cagcaccagt tggccgaagc ttataaacag gccgccggcc agcaacgccc gcgcgaactg
     1621 atcggccaga gcgccgtgca caagcgcctg caggcggaga ttgcgctagt cggcaacagt
     1681 gaactgagcg tgctgatcag cggtgaaacc ggcgtcggca aggagctggt cgccgaagcc
     1741 ctgcacgccg catccccgcg cgccgccaag ccgctgatca gcctcaactg cgcggcactg
     1801 ccggataacc tggtggaaag cgaactgttc ggccatgtac gcggcgcctt taccggcgcg
     1861 cacaacgagc gccgcggcaa gttcgaactg gccgacggcg gcagcctgtt tctcgatgaa
     1921 atcggcgagc tgccactgcc cgcacaagcc aagctgctgc gcgtgctgca gagcggccag
     1981 ctgcaacggg tcggtgccga tcaggaacag cgggtcaacg tgcgcgtgct ggccgccagc
     2041 aaccgcgacc tggccgagga agtgcgcgca gggcgttttc gcgccgacct ctaccaccgc
     2101 ctaagcgtct accccctgaa agtcccggcg ctgcgtgaac gcggccgcga tgtgttgctg
     2161 ctgaccggct atttcctcga agaaaaccgc gcacgcctgg ggctgcgcag cttacgcctg
     2221 actgccgacg cccaggccag cctgctggcg tacaactggc cgggcaacgt gcgcgaactc
     2281 gaacacctga tcggccgcgc cgccctcaag gccctggccg gatgcagcga acgcccacgc
     2341 atcctcagca tcgacagcgc ccatctggat ttgccgacca gccaacccgt cacagcgcct
     2401 gcaactcaac gcgccgcaga gccacacagc gagctgccgc tacgcgaagc cctcgacgcc
     2461 taccagaaac agctgatcag ccaggcgctt gcccgccacc aaaacaaatg ggccgccgcc
     2521 gcccgcgaac tgggcatcga ccgcgccaac ctaatccgcc tggccaagcg attgggtttg
     2581 caaaattaat cactcattac cccacgtcgc gccacacaac tgcagctacg tggctaatct
     2641 tcaaggcggg cgcggcactc gctcggcgtg atgctaaaag cccacgccac caataggcaa
     2701 aagcaatttg ctgcccgcac tgcatgggta gctaatggcc agcactcagc tactccctct
     2761 ggagagcctt catggccgac gaagccaaac cggaccgcat caaagtcgac gcagcgcaac
     2821 tgaccatcgg catgtatgtg gtcgaactgg atcgcccatg gacggacacc acctttcttt
     2881 tccaaggctt tcgcattcgt cagcaacagg acattcgcct gctgcaggaa gtctgcagtt
     2941 acgtctgggt ggatgctcgg caatccgtca ctgtgcatga tcaggtcacg gccaatatcg
     3001 cccagtctga ggcgctgcaa cccgtcatcg ccaaggtcga tttcaacctg gaaatgctcc
     3061 aggcagcccc cacctggaac gctgcgcgcg aagagtcgtt gcggattctg caggcggtca
     3121 aactgggtca ggagctggat gttgaggcgg tcaaagccgt catcaaggac tgtgtcgaaa
     3181 gcatcctgcg caacccagcc gcgatgctct ggctggcgcg catcaagaac agcgacgaat
     3241 acaccgccga gcattcgctg cgcgtgtcca ttctttcgat tgccctggcc aaggagctgg
     3301 gcctgccggt ctatcagctt gagcaaatcg gcgtatgcgg catgctccac gatgtcggca
     3361 agatcaaggt gcccaacgaa atcctcaaca aacccggcgc gctgaccgtc gaagagctgt
     3421 gcatcatgca gagccacgcc gccgagggcc gcaagctgct gatgagcaac cagcaggtca
     3481 ccgccgccac cgtggatgtc gcctactcac accatgaacg cctggatggc cagggttatc
     3541 cgcgcggcct gaatgccagc aagatccctt acttcgccaa gatcatcgct gtggccgaca
     3601 gctacgacgc catcaacagc gaccgcgtat acagcaaggg caaatccagc ctggaggcac
     3661 tgcgcatcct gctggaagcg gtcaacagcc atttcgacga agagattgtt ggctgcttta
     3721 tccgcatggt cggcatctat ccgccagggg aaatcgtcga gctgaccaat ggcgaagtcg
     3781 ccatcatcat cggctgccca cctggcaaca agctcaaacc aagggtgtta cgcgtgctcg
     3841 acgggcagaa gcaaccttgc aaggaaagca tcatcgacct gggcaaaacc gccaacgacc
     3901 ccagcggcaa accctaccga atccacgaag tgcacagcag cggagccttc ggtattgata
     3961 tagatgcctt ccgcagcaag ggactggtaa tcccgacgca cctttaaaaa gctcaaaaac
     4021 gcaaaaagcc ccggattttc atccggggct ttttgcttta aaggtggtgc accaggcggg
     4081 attcgaaccc acgacccctg ccttcggagg gcagtactct atccagctga gctactggtg
     4141 cagcgcgggc gcaatcatac gcatctcgct ggcgggcgtc catgctgctg ccttgttcgc
     4201 aattctgaac ggtggccgct ggccagctgt caatctatat acggcgggtg aataagcgca
     4261 atattccgtt aattttttcg aacagactct tgccctttac ccctcatgac cctaggattc
     4321 gtttgagatt tcaaacgctc ttgctaaaac tgtgtgtaat tccggctgat tggtcatccg
     4381 cgaggcatac atcagcagca agggtgtaca caccgaacct acacgcgccg cttggcggcg
     4441 tacccagcgc aagtgccgat accagggtgt cggaatttag gagactgcca tgcaactgaa
     4501 agacacccag ctgttccgtc agcaggctta tatcgacggc acttggctgg acgccgacaa
     4561 cggccagacc atcaatgtca acaatccggc caccggagaa atcatcggca tcgtgccgaa
     4621 gatgggcgca gcggaaaccc gtcgcgccat cgaagccgcc gaccgtgcgc tgccggcctg
     4681 gcgtgccctg accgccaaag agcgctcgaa caaactgcgc cgctggtttg aattgctgat
     4741 cgagaaccag gacgacctcg gtcgcctgat gacgctggag cagggcaagc cgctggccga
     4801 agccaagggc gagattgtct acgccgcttc ctttatcgag tggttcgccg aagaagccaa
     4861 gcgcgtatat ggcgacgtga tcccgggcca ccagccggac aagcgcctga tcgtgattaa
     4921 gcagccaatc ggcgtaactg cggccatcac cccttggaac ttccccgcgg cgatgatcac
     4981 ccgtaaagcc ggtccggccc tggccgccgg ttgcaccatg gtgatcaaac cggcttcgca
     5041 gacgccgttc tctgctctgg ccctggtcgc actggccgag cgtgccggca ttccgaaagg
     5101 cgtgctgagc gtggttaccg gcagcgccgg cgaagtcggc ggtgagctga ccagcaaccc
     5161 gatcgtgcgc aagctgagct tcactggctc cactgaaatc ggtcgccagc tgatggccga
     5221 atgcgccaag gacatcaaga aggtctcgct ggaactgggc ggcaacgcgc cgtttattgt
     5281 gttcgacgat gccgacctgg ataaagccgt cgaaggcgcg ctgatatcca aataccgcaa
     5341 caacggccag acctgcgtgt gtgccaaccg cctgtatatt caggacggtg tgtacgatgc
     5401 cttcgccgag aagctggccg ccgccgtggc caagctgcag atcggcaacg gcctggatga
     5461 aggcaccacc accggcccgc tgatcgacga caaagccgtg gccaaggtgc aggagcacat
     5521 cgccgacgcc ctgagcaaag gcgcgcgcct gctcgctggt ggcaagccgc atgcgctggg
     5581 tggcagcttc ttcgagccga ccattctggt cgacgtgccg aagaacgccg ctgtggccaa
     5641 ggaagaaacc tttggcccgc tggcgccgct gttccgcttc aaggacgagg ccgaagtgat
     5701 cgccatgtcc aacgacaccg agttcggcct ggcctcgtat ttctatgccc gtgacctggg
     5761 tcgcgtgttc cgtgtcgcgg aagccctgga atacggtatg gtcggcgtca acaccgggtt
     5821 gatctccaat gaagttgcgc cgttcggcgg catgaaagcc tccggcctgg gccgtgaagg
     5881 ctcgaagtac ggcattgagg attacctgga aatcaaatac ctctgcctgg gcatctaacc
     5941 gggcgacgta gcagtaaccc cgccgaagac gcacgagagc gctgcgcctt cggcttttat
     6001 atggacatac cttgtggccg ggcatgctgt gacagtcgat catcgaatac tgccaagccg
     6061 ccgtcggtga cacatccttg aaccacgccg agattaaccg gcgaatgagg acatcatgag
     6121 caagaccaac gaatctttga tgcaacgccg caccgctgcc gttccacgcg gcgtgggcca
     6181 gatccacccg atcttcgccg agtcagcgaa gaacgccacc gtgaccgacg ttgaaggccg
     6241 cgaatttatc gacttcgccg gcggcatcgc ggtgctcaac accggccacc tgcacccgaa
     6301 agtgattgct gccgtgcagg aacagctgac caagctgacc cacacctgct tccaggtgct
     6361 ggcctacgag ccttacgtcg agctgtgcga gaaggtcaac gccaaggtgc cgggcgactt
     6421 cgccaaaaag accctgctgg tgaccaccgg ctccgaagcg gtggaaaacg ccgtgaagat
     6481 cgcccgtgct gccactggcc gtgccggcgt gattgccttc accggcggct accacgggcg
     6541 cacaatgatg accctgggtc tgaccggtaa ggttgtaccc tactcggccg gcatgggcct
     6601 gatgcccggc ggcatcttcc gcgcccagta cccgtgcgcg ttgcatggcg tgagcacgga
     6661 tgacgcgatc gccagcatcg aacgcatctt caagaacgac gccgagccgc gcgatattgc
     6721 cgcgatcatc atcgagccgg tgcagggtga gggcggtttc tacgtcgcgc cgaaagactt
     6781 catgctgcgc ctgcgcgcac tgtgtgatca gcacggcatc ctgctgatcg ccgacgaagt
     6841 tcagaccggc gccgggcgta ccggcacctt cttcgccatg gagcagatgg gcgtcgccgc
     6901 cgacctgacc accttcgcaa aatccatcgc cggtggtttt ccggtggcgg gcgtgtgcgg
     6961 taaggccgaa tacatggacg ccatcgcccc aggcggcctg ggcggcacct acgccggcag
     7021 cccgattgcc tgcgccgcgg ccctggccgt gatggaggtg tttgaggaag agcagctgct
     7081 ggatcgctgc aaggccgttg gtgagcgtct ggtcactggt ctcaaagcca ttcaagccaa
     7141 gcacaacagc atcggcgaag tgcgtgccct gggcgccatg attgccatgg agctgtgcga
     7201 aggcggtgat gtgcataaac ccaatgccgc gctgaccggg cagattgttg ccaaggcgcg
     7261 tgagaaaggt ttgatcctgc tgtcgtgtgg tacttacggc aacgtgttgc gcgtgctggt
     7321 gccgttgacc gctgaagacg ccctgctcga ccgtggcctg gcgattatcg ccgagtgctt
     7381 cgacgaactg gcgtaagccc acagcgttag cccggcaata aaaagacccg cttcggcggg
     7441 tcttttgctt tcggcgattg ggcaggtcat cgaattgata gcccagcgca tgagccgacc
     7501 cgccactaac gacctgaagt atcgagcacc aacttcatct gtaactcctc aagttggctg
     7561 tcggccaact gagtcagcgg ctgcgggtgg ccaataaaat aaccctgggc atagtcgagc
     7621 cccagcccgc gcacgcagtc cagggtggct tggctttcaa caaactcggc cacggtgctc
     7681 aggcccagct ggctggcaat ctgttgcatc gagccaacca tggcctggtt gatcgggtcg
     7741 ctctccaggc cgctgataaa gctgccgtcg atctttacga tatccagcgg caaatggcgc
     7801 aggtaagcgt aggaggcaaa accactgccg aagtcatcca gcgccacttt caggcccagg
     7861 ctgcgcagct gtttgatcca ctgcgatgac acccccagct cgcccagggc gaccatttcg
     7921 gtcacctcga tgcacagctt gtgcgccggc aggttgtgcc gcagcaattc gctatggagc
     7981 atctcgtgga agtgcgcgtt aagcagcgag ctggcggtca ggttgaaatt cacctgagcc
     8041 aattgctgca catgccgcgg gttgtccgcc agccattgca gcaggcgcat caccacccag
     8101 cggtcgatgg caccgagaaa gccgtagcgc tcggcggcac cgagaaattg tccggggctg
     8161 acccactcgc cgctgatcgg gtcgtggtag cgcagcagca cttcatagcg caggccatcg
     8221 ctggcgttct gcagcgccat taccggctga tagaacaact cgaagtggcc cagttcgatg
     8281 gcctcgcgca ggcgggtgat ccattgcagc tggcgttgat gctccagcaa cacgccatcg
     8341 gccgggttga actgctgcac ccggttacga cccaggtgct tggctagctg gctggcacta
     8401 tcggcccagt tcagtgcggt ttcccagtca ctaacgccgc tgtccagttc cagcaagccg
     8461 atgctggcct gcacgcggaa cggccgaccc tgccagctga agacgaattg ctcgacacag
     8521 tggcgcaggc tttccgcatg ctccagggct gcagcgctgt cgacctcacg cagcaacacg
     8581 gcgaattcat cgccaccgat acgcgccagt tcggcatgcc gtggcaactg gtgtaacaga
     8641 tggccggaca actggcgcag cagctgatcg ccggccgaat gcccgcagag gtcgttgacc
     8701 tgcttgaact gatccagatc caggcacagc agatggctgt agctcggcgt gtcctggcct
     8761 ttgaggcttt cacgcagcag gcgttccagc tcgcgacgat taagcagacc ggtcagcgag
     8821 tcatgcgccg ccaggtactg cagccgctct tccagagcgc ggcgttcgct gaggtcgacc
     8881 acgatgcctt agatgcaatc ctgggtcggg cgcagatgca acgacagata gacccaatgg
     8941 ctctgcccgt cgagcccatg aatctgacac tcctggctga tcgccggctg ctcggtatgc
     9001 agttgcgcca cctgctctgc ccattgctcg acgcccaata gctgctgcat ggacagctca
     9061 ctgctgtcct gcgccaaccc cagcaacgca gcaaaactcg gattggcttc gatcaacgca
     9121 ccgctgcggg tgcagcggaa aatcccctcg ccactgctgc tgaacagcgc ctggtactgt
     9181 tccagattgc ccagcgcctg ggccttgctg tgcagcaggg cgcggcggat ctgctccagc
     9241 tgtttgtcga ggatgccgcc aaacagcagc atgctcagca ccggcaggta ggcgtgcatg
     9301 taattgaagc tcggcggcag cggaatcacc ccgctgcgcc ccagcggcac cagcagcatc
     9361 agcagcaggg ccgaactcca gcacagcagg tagccgatgg cgtaaggccg gcgctggcgc
     9421 acgccgagga tcagcagcaa cagctggaac gccccgttga gcagaatcag cacgttcagc
     9481 gtctggtagg agcggaagtg cccatagctg acgatggtcc agccgcacac cagcagggtc
     9541 acggcaaaca gtgcgttgcg cagatggcgc acgcggccca gctcgagctt cagtgagtcg
     9601 gccaaaaaca ggctgctgaa gatcagcatg ccggggttgg ccagattggc catctgggtc
     9661 gacaaccaca cccactgcgg cgccagcagg ctggtaacgc catgcaggct cgcgttgtac
     9721 agcgccacgc tggccaccgt caggcagaac cacaccagct gtgtttcgcg cagggcactg
     9781 aatttcacca gaaagaacag ggtcaggctg agcagcgctc ccagcaacaa gccgctacgc
     9841 atccagtgtt cagccagcaa taacgggctt tgctcggcgg ccaccagttg catcggtaca
     9901 ctcaccgccg agccgctttg cacccgtagc aacaacgtat gcggaccttc gccaagccgc
     9961 ggcaggttga gcagaaagtg cggcgagggc gcgccacgca aaagaaaatc gcggtgatcg
    10021 ccactataga aaggggcgat cagccgttca cggtcatata gccagacttc gatatcgtcg
    10081 agaaagggcc gatcgatcat cagatcgagc cgttcggcca ccggcgcatc aaggcgaaac
    10141 gccagccacc aggcgctggt cgaatacccc aggcgcggca caccatcaat tcgcttgccc
    10201 tcgctggcaa tacgttgctg cacctcggcg gcggtcaggt tgccactggg gtcttccagc
    10261 accaggctgg caggaacctc caccggctgc ttgagctgct cgctactgag caccagcagc
    10321 ggcagtgttt cggcatgtgc cgaaacactg cccagcgcaa agcagagcag agcagccagc
    10381 aagggaaggt tgaacagatg gagcatcctg gcgcctcaat aaactgcgag gtgcgccaag
    10441 gctcagggtg aacacagccc gcctgctagg cgagttgacg ctgaggaatt ctagctgaga
    10501 actggcgatt tgattgcgct gacggtctca cctagctcca gtagcaggcc ggtaatttgc
    10561 tcggcactca tgctgaaggg atcaaaacgg tcgctggccg aatatttcag aaacaactcg
    10621 cggttggcct gcttggcgcc agcccagccc atgctccagt tgctgtaacg gcgcatgctg
    10681 atttcggcgt agtcgaggat agtcaggtcc ttgtgccggt tgtcatcggc caaccggttg
    10741 tacaaggcgt tcacctccgc acgcggccct tcgagtgcct gcagaaagat atcggcgttg
    10801 cagcagagta tcccggtcaa gcctttacct gggttgttgc gctgtgaact gtcgagaatc
    10861 tcgcggatta gctcggaaga aacgccgtga ctggcacggc tggcgtaggt aaggcggact
    10921 aacataaacg gatcctcgca ggcggcgggc agggcaacag ctgcagattc tgcattatct
    10981 gtataagatt tgtataacta attacgcaac attcttctac ctgcgcgcgt aacaaaaacc
    11041 ctccggcact ctctatagtg gctacttagc ggctgacacg atcagccggc gcgtcagcat
    11101 caaggaaccc aacaccgtgc atgcaatgtt gcccttgctc gaccaactgg cttttccggc
    11161 aatccgccgc ggccaactgg aagcgttgca gatcaacctg acctatcgct gcaaccagcg
    11221 ctgcctgcat tgccatgtga atgccgggcc gacccgcacc gaggccgtga ccgacgaaag
    11281 cctgacgctg ctgcatcagg tgatcgacgc ccatccggta cataccctcg acctcaccgg
    11341 cggcgcgccg gagatgcacc cacgttttcg cgagatcgtg acccacgcgc gccgcgaagg
    11401 cctgcgggtg atcgaccgct gcaacctgac catcttgagt gagccgggtt atgaagacct
    11461 ggcgcagttt ctcgccgacc agggtgtgga agtcagtgct tcgctgccct gctattcacg
    11521 cgacaacgtc gacaagcagc gcggcgacgg cgtgttcgat gccagcatca agggcctgca
    11581 acagctcaat gccctgggct acggccaacc ggacagcggg ctgattctca acctggtgta
    11641 caacccgcaa ggcccgagcc tgccgccacc gcaagcggcg ctggaagccg actacaagcg
    11701 ccacctggcc gaggacttcg gcattgtctt caaccacctg ctgaccatca ccaaccagcc
    11761 gattgcgcgc tttggcagca ccctggtcag caagggccaa ttcaacggct atatgcagtt
    11821 gctgcgtgac agctatcgcc cggaaaacct cgaaccgctg atgtgccgca gcctggtcag
    11881 cgtcgactgg cagggctacc tgtacgactg cgacttcaac caaatgctcg atttgccgtt
    11941 gcaactgcct gaagtgatcg gccgtgatcg gccgcacctg cgcgatctac tcgatgcaac
    12001 gctggccggc aaccacatcg ttacccgtga tcattgttac gcctgcaccg cgggccaggg
    12061 gtcaagctgc ggcggcgcgc ttaatgacta accccgtagg atgcgctgtg cgcaccaacg
    12121 caggtgatga acccttggtg cgcacagcgc accctacgcg actgcataac cattaattaa
    12181 ggatcggtcc atgcaaccca ccagtctcac cgggctgttt ttctggggtt ttctcgtggt
    12241 ctacggcgcg ctgatgctgg ccctgtcgcc acgcgcggta accctcggcg ggttcttcaa
    12301 tggtgaggac aaccagggcc gcagcgccag cccctggctg atcacctcca gcatttttat
    12361 cagctggatc ttcgccaaat cggtaaccaa cgccgccaat ttgggggcca cttacggcct
    12421 ggtcggcggc ctggcgtatg ccatgtactg gctgtcgatt ccgctcaccg gttttgtcat
    12481 ctatcgcctg cgtcggcgct ttgccgccac cggcctggtg aactttctca gcagccatta
    12541 cggccgcggc gcagcgctgg cgttttccgc ggccattctg attcgcctgt ttaacgaggt
    12601 gtggagcaac actgcggtgg tcggtggcta ttacggcgac tcgggtagca tcgaatttat
    12661 cgcctcggcg ttgctgttta ccgccgtgac cctggcctac agcctgcgcg gcgggctacg
    12721 cagctcgatc cttaccgacg cggcgcaagc ggcgatcttc gtgattgccc tggtctgggt
    12781 gctcggcctg gtgctgccgc agcattcgcc gacagagctg gccagcacca gccattgggc
    12841 gctgaatgcc ggcgtcgacc tactgctggt cgccggtctg caggccttca gctacggctt
    12901 ccatgacccg gtactgaccg accgtggttt tatcagcgag gagaaggcca tgcgccgctc
    12961 ctttctgatt gccggcattc tcggctttat cgccatcctc gccttcagcc tgatcggcgt
    13021 acatgcgcag ctggtgggca ttgccgcctc ggacaatgta ccggcggcct tggccaagag
    13081 cctgggcatt ggcgcgctgc tggtcatgac cgtggtgatg gtttccgctg ccggctcgac
    13141 cctggactcg accttctcca gcctggccaa actggccggg cgcgagctgc cgagactggc
    13201 aggccgcgac ctcgggcaga aagccatagg cgtgggcatg gcggttatgg tggtgttcgc
    13261 cctgctcggc aacctgccga tgatagccgg caccgacata ctcaaggcca ccaccatcag
    13321 cggcaccatg gtcattggcc tggcaccggt gtttgtcctg cacggcctga ccacgcctac
    13381 ccgactgggc tttcatctga gcttctggac cggcctgggc ctcggcgtcg ccctgaccct
    13441 gaactggatt ccgcaaagct gggccatagg cgacggtaaa tacgcgctgc tactcggcac
    13501 caacctctat ggcctgggcc tctgcgtgtt ggggtatctg ctgcccagct ggctacggag
    13561 gactgcctga tgcggcttgg cctcgtaggg tgcgctgtgc gcaccaagag tctgcctatg
    13621 gagttggtgc gcacggcgca ccctactgct gttggggccg aagcctgatg cgcgccggcc
    13681 gcgattgtcc gctggattac cgtctggcgc gtgatgcctt tagcggtgag ccgctgttcg
    13741 actgcgacat gctctatgtg gtcggcggtc tgtatggcaa tcagcaggcc ttggccgcac
    13801 tggagaagcg cctggcagcc gaagccggtg cgcggctggt attcaacggc gatgcccatt
    13861 ggttcgaccg cgatcccgag attttccaaa ccatcgagca aggattgaat ggccacctgg
    13921 ccttgcgcgg caatgtcgaa accgaattgg gacgtgccga cgacagcggt gccggctgcg
    13981 gctgtgctta tccgctaacg gtggaggagg cggtggtcga gcgctccaat gccatccagc
    14041 ttgagttgaa taaaaccgtg cagggtttgc ccggtatagc cgaacgcttg gccacgcggc
    14101 cgagcaccgc gatagtcagc gtggccggcc agcgcgtggc gattagccat ggcgatgagc
    14161 aatccctggc tggctggggc tgctcgcggg agtcgctcag agagtcggag cgccagcagc
    14221 aactggacac ctggttttcc acgaacaatg tacaggtgct cgccaccagc catacctgct
    14281 cggccgtggc tctgaacctt gagcacggcg cgctgatcaa caatggcgcg gccgggctgc
    14341 cgaactttgc tggcggccgt tatggcctgc ttagccgtat cgccaccacg ccacatccag
    14401 cggcgctgta tcgctgccag cgtgacggcc tgtttatcga agccctgccg ctgaactacg
    14461 accacactgc ttttctcgcc gacttcaacc gccagtggcc tgccgatagc ccggcggcgt
    14521 tgtcctatcg ttcgcgggtg ctcggccaac tgaccgaccg ccccgagact gccctgctgg
    14581 ccgattttag cctgtgccaa agcctgtgcg ccctggaggc gctgactgat gaatagccgc
    14641 aaactcgccc tgatgggcct gatcctcgcg ctgctgggca gcttctttgt cttcgacatc
    14701 ggccagtacc tcaatctgga agcgctcaag gcgcagcaag cggcgctgaa tgctcaagtg
    14761 gccgcgcagc cctggctagc agcggggctg ttctttctcg cttacgtcgc ggtcacggcg
    14821 ctgtcgctgc ccggcgcagc cttgatgacc ttgctggccg gcgcgttgtt cggcttgctc
    14881 gaaggctttg tactggtgtc cttcgcctcc accctgggtg cgaccctggc tatgttgagc
    14941 agccgttttc tgctgcgcga ttgggtgcaa agccgcttcg gccagcgcct ggcgggcatt
    15001 gacgcgggca tcgaacgcga gggcgcgttc tacctgttcg ccctgcgtct ggtaccactg
    15061 tttccgttct ttctgatcaa cctggccatg ggcctgaccc gcctgccggt gcgcacctac
    15121 tggtgggtca gccagctcgg catgctgccc ggcaccctgg tttatgtgaa tgccgggcgc
    15181 gagctgggcc agctcgactc gctggccggc atcctctcgc cgggcttgct cggtgccttt
    15241 gttttgctcg gcgtattccc gttgatcgcg cgcaagctgc tgggcctggt ccaggcacgc
    15301 cgggtatatg ccggctggca caaacccaag cgcttcgagc gcaacctgct ggtgatcggg
    15361 gccggggccg gcggcctggt cagcgcttat atcgccgcgg cggtaaaggc caaggtcagc
    15421 ctgatcgaaa aacaccaaat gggcggcgac tgcctgaaca ccggctgcgt gccatccaag
    15481 gcgctactgc gttcggccaa gctggccaat gaactgaaaa agggccaggc gctgggcttt
    15541 agcggcgtgc agggcacggt ggatttcccg gcggtgatgc aacgtatcca gcgggtgata
    15601 gccgatatcg aaccccatga ctcggtggag cgctacacca gcctgggcgt cgaggtcatt
    15661 cagggcgagg cgaagattct ttcgccctgg agcgtggagg taaacggcca gaccctgacc
    15721 agccgcaacc tgattatcgc cgccggcgcg cggccgctgg tgccgaagat tcccggtatc
    15781 gagcaggtgc agggctacac ctcagacagc atctggagcc tgcgtgaaca gccgcgttgg
    15841 ctgctggtgc tgggcggcgg gccgattggc tgcgagctgg cccaggcctt ccagcgcctc
    15901 ggcagccagg tgattcaggt ggaactggct gaacgcctat tgccgcgcga agacgccgat
    15961 gccagcgagt tggtgctgag cagcctacgc gcagatggcg tggatgtgcg cctgcagcac
    16021 cgcgccgagc gcttcgaggt ggtggacggc gagcagcgta tggtcgcccg tcgcctggac
    16081 accggcgagg aggtgaccat cgccttcgac ggcttgctgc tggccctcgg ccgggtggcc
    16141 aatgtcacag gctatggtgt ggaggaactg gggctgaccg tacggcccaa cggcaccctg
    16201 gaaaccgacg aatacctggc cacgcgcttt cccaatatct acgcggtggg cgatgtcacc
    16261 gggccttacc agttcaccca tgtcgccgcg caccaggcct ggtatgcggc agtcaatgcg
    16321 ctgttcagcg gctttaagcg ttacaaggtg gattaccggg taatccccgc ctgcaccttt
    16381 accgaccccg aggtcgcccg cgtcgggctg aacgaaggcg aagccaaggc gcagaatatc
    16441 gcctatgagg tgacccgcta cggtatcgac gatctggatc gggcgattgc cgatgaagcc
    16501 gcccatggct ttgtcaaagt gctgaccgta ccgggcaagg accagattct tggcgtaacc
    16561 atcgtcggcg aacacgccgg cgagctgcta gccgaatacg tgctggcaat gaagcatggc
    16621 ctgggcctga acaagattct cggcaccatc cacacctacc caaccatggc cgaggccaat
    16681 aaatacgccg ccggtgaatg gaagcgcacc catgcaccac aggggctatt gcgcctggtt
    16741 gagcgttttc ataactggcg cagaagctga tgcgcgggtt actgctcgcg ctgctggtac
    16801 tgacgccgct ggcccaggcc aataaccagt tgacgccctg gacggcaggt ccgcccaacg
    16861 acgaatggtc accgccctgg cgcctggcgg atatccccaa gctcaagccg tccgagcgca
    16921 gcctggtcga gcaagacaga ccgcaggtgc tgcggatcaa ggccgaggcc gcagccggtg
    16981 gcctgttgca tccgctggat ctacccggcg atcagccctg gcaactgagc tggcaatggc
    17041 gcactgagca gaaactggag caggcgcgct tcgccacccg tgaaggcgac gactacgccg
    17101 cacgggtcta tgtgctgctg gattacccgc tggattcgct gagttttgtc actcggcaga
    17161 agctgcgcct ggcgcggacc ttcttcgatc ccgacctgcc ggcggcgacc ctgagttacg
    17221 tctgggataa ccgcgcagcc cccggtatcc atgcccgcag tgcctatacc gagacggtgg
    17281 agatgatggt gctgcgtggc ccggaagcgc cacttgggca atggctcagc gaaagccgca
    17341 atctaaacgc cgatgctatc gccgcctttg gcagcgcgcc acgcaagatc ctcggcgtgg
    17401 tgtttgccac cgacagcgac aacaccggcg agtcggcaga aagctatttc gccgcgccgc
    17461 agctgcagtt atctaatccg taggagcggg ggggacgccc agttccatgc ccgcgaaacc
    17521 tgcaacacga aagcatcgcg ggcaagcccg ctcctacatt cagaactcaa gcgacctgag
    17581 cctgcttggc gggctcagcc tgctcgcgac gcgtccagcg attggcccgc gcaaaagtgc
    17641 cgaagtcgtt aaaacgcacg ccctgttcac gcatcacctt atgcgcaaac ggcacgctta
    17701 gctgacggat atagaagggc tccttgacca caaaatggtg gatcgcgtgg ctgctgccga
    17761 agttgaagca gaacgcctgc agcggccaca accaccaggg gttgagcacc tgggtctgct
    17821 ggatcacatt gcccgcctcg acatcgccgt agtaatgcat gttcgagctg acaaagtgca
    17881 ggcagaaggt acgcagcacg ttggggccga ccagcaccac cacggcgata tttaccactg
    17941 ccatcacgct cagggtggtc gccgaccagg ctacgggggc acccagggcg gtggccgcaa
    18001 agtcgatcaa atggaaaccg agaaacacat accaggtgcc ccaattcagc aggcccagcg
    18061 gcgcgtagac cttcagcgtg cgggtcagaa tcagtcggcg gtgtttggga ttgctcgccc
    18121 gtagccagcg aataaacgag ctcatcatgt tgtcgccgac catcagcagg cgggcaaagc
    18181 cccagggctc gccgttggta atggcgcgct cttccatatc ctgttcactg ccggacacct
    18241 tgtgatggtt aaggtgcaga tggcgacgca cccaggggtt gatggtgctc ggccgcgcca
    18301 gccagaccag cgccatcatc aggttgtgcg gcacccggcg cttgcggaag tacatggagt
    18361 ggatcaggtc gtgctccaac tcatgggtca gcgaggcgaa gaaggcattc agcagcaggc
    18421 aggcccacca ggccaggtaa ccgccgatat acaacgccgc cgaaccgatc atcccggcca
    18481 gggcgaaggt gaggatgccg gcaccgaggg cgtcctgatg attgaggatg gggaagcgct
    18541 gacgcagttc gacaccgtgg gccatgacct gttggcggat cagcgcggcg cgttgcgcgt
    18601 catttaatgc tgcagtgctt gcaggggagt ggggcatgcc tacatcctct tgttgatgag
    18661 gtggccactg tgccgcgaag cccgggcctg cgcctgaccc tgaacgccaa cctgttgacc
    18721 ggatccgcca atctgcatga ctgccgaacc caccaccctg gccagttgga cccgcgcact
    18781 gcgcaagcag ctcgatgcgc tgggcctcga cagcgcggcg ctgtgtctga aggccgggct
    18841 tgagccgagt ttgctcgacg accccaacgc gcgctgcccg ctgtccatca ccacgcgctt
    18901 gtggcaactg gcggtcgagg ccagcggcga cccggcactg ggcctgaaaa cctcgcagtt
    18961 cgtcagcccg accacctttc acgccctcgg ttacgccctg ggtgccagca gcagcctgcg
    19021 cgagatgttc gagcgcatcg tgcgctatca ccgggtggtc agcgatgccc tggagctgga
    19081 gctgcgcgag gccggtgact gctacgaatt ctgctttcgc gtaccgcccg gcagcccgcc
    19141 gccggccccc gaagcgctgg atgcctttgc cgcgatctat gtgcgcagct gccgcaaccg
    19201 cctgggccgc gacttcgccc cgctggcggt gcagctgcag cgcgccgagc ctgcggatgc
    19261 acagccctgg catgcggtgt tccgcgcgcc gctgctgttt gccgcgccgg aaaacctcct
    19321 gcgctttccc cgcgccgctt tcgaacagcg cctggacgac ggtaacccgg aactggccga
    19381 gcacaatgaa acggtgctca agcgcaccct cgaacagctg catcacgcca cctggacgca
    19441 caaggtgcgt gcctgcctgg aggcccagtt accggacggc gaaccctccg ccgagcgtat
    19501 cgcgcagacc ctgcacctga gcctgcgcag cctgcagcgc catctggccg acgaaggcgg
    19561 caactatgaa ggcctgctcg ccgacacccg ccacagcctg gccctgcaac atatgcacga
    19621 cccgcgctgc tcgatcagcg agatcgccta cctgctcggc ttcgccgaca ccagcagctt
    19681 cagccgcgcc tttaaacgct ggactgggca agcgcccagc cattaccgcg atggactgaa
    19741 acaaccatga agcaatacga cttgctattg ataggcgccg gccacgccca cctcggcgtg
    19801 ctgcggcgct gggccttggt cgagcggcca ccggggcgca ttgccctgct aagcgccgga
    19861 ccggtggcct ggtattccgg catgttgccg gggctgctgg ccgggcggca tcggccggag
    19921 gactgtcagg tcgagctgca ggcgttatgc cgcgcagcca aggtcgagct gatcatcagc
    19981 ccagtagaaa gcctgtccgc tgcccagcag caggtgcaac tggcagatgg gcaaaccctc
    20041 agcgccagct ggctgtcgct caatgtcggc gcgcaagttt ctgcaccggc gcagcaaggt
    20101 gatgccatgc agttgctggc ggtaaaaccc tttgcggaat ttcttgaggg ttggcagcgc
    20161 tggcaggctg agccgcagcc cctggcgatt ctcggtggtg gcgcagcggg ggttgagctg
    20221 gcgctggcct tggccggaaa ggtgccgcag ctggcgctgt tctgcgctgg tcatctgctc
    20281 gatgggttat ctgctggcgt acgcctgcgt gccctcggcc atctgcgcca acgcggcgtg
    20341 tgggtacgtg aggattgccc gatcagccgc attgcggacg actgcctgct cagcggcgac
    20401 gagccggtct ggcgcggtca acggctgtta ctggctagcg gtgccaaggc ctttgcctgg
    20461 ccggcgcata gcgggcttgg ctgcgatgag cagggctttg tactgattgc gccgacgctg
    20521 cagagctaca cccatccgac gattttcgcc gtgggcgact gcgccagcct gcccggcgcg
    20581 cgcaagagcg gggtgtactc ggtgcgtcag ggcccggtgc tagccgccaa cctcaacgcg
    20641 gcgctacgtg gcctggcgct gaagcgctac cgtccgcagc gacagagcct cgccctgctg
    20701 gccaccggtg atggcggcgc gctgctggac tggcatggcc acagcgccgg cgggcgcttc
    20761 tacgggcgct ggaaggattt cctcgacaag ggatttatcc agcgtcatcg tctttaggca
    20821 ctaacccctc tgcgaccaaa gtgccatggc cgaataccgc catgggtgct cgaatcaacc
    20881 tcccaccaaa ggttggccgc agggagcgtc acccgctatg gtctatagat tcgccgacaa
    20941 cggccgattg cctacccagg cacatccagc tgcacgcact ccggtttcag caggcatggc
    21001 tcgcccaccc cacagcggcg cgccacaggc agtttctggt ggtggctcga ccagaataaa
    21061 aataatgagg tttgtcatgc gctctgcgtt tattacctat cccaaggcct gtctgctgca
    21121 tgccatcggg ctggccgggt taatgctgac gtaccagcag atcgaactgc cgtggtttgt
    21181 cagcattccg tcctttctgt tgctggccct gtggccgtgg ctcgggccgt ggcagcgcca
    21241 gcatgaagag caaccagcgc atgctaaacc acacgacacg ttcagcgacc tgagccagag
    21301 cctatcgcga cagacctgtc acaacgccct ggccgccgcc gaggtggccc atgcagcgca
    21361 acagctggcc ggcaaactgc aatcgcagct cagcgccacc gagcagatca accaggccgc
    21421 cgaggccatc acccataccg aacaggacaa cgcccagcgc gccgagcaaa ccctgctggc
    21481 cgcgcaaagc gtgcgacaga acagcagcag cggccagcag gaactgcgcc aggccatcga
    21541 gaccatgcag cacctgagcg cacagaccgc caccagccgc gagctgattg acggcctcag
    21601 cgcacgcacc gagcagatcg agaaagtcac ccaggtaatc cagtcgattg ccagccagac
    21661 caacctgctg gcgctgaatg ccgccatcga agccgcccgc gccggtgagc tcggtcgcgg
    21721 ttttgccgtg gttgccgatg aggtgcgcaa cctcgctggc cgcaccagca gcgccaccga
    21781 agaagtcggg cagatcgtca gcgatatccg ccagcagagc ctggcggtgg tcagccatat
    21841 gcagcaacag gccgaacagc tgacgctggc cgcacaacag gtcgagcaca ctggcgagca
    21901 attgcacgga attgccgaac tggcggtgga cgtggaaagc caggtcagcc agatcgacag
    21961 cggcacccgg caaaaccacg agcgcctggc cgaactgtcc gtggcggtcg gccagctgcg
    22021 cggcgatgtc agcgacagcg aagggcagac gcgtcaactc agcagcgccg ccgaacggtt
    22081 agtcgcacag gccgaaagcg tcagcgaaca gctcgccgaa gtcggcctgg atgaatacca
    22141 ccaacgcgtc tacgacctcg ctcgtagcgc ggcagcgctg attgccgcac agtttgaagc
    22201 agacctgcac agcggccgga tcagcgccaa cgacctgttc gaccgtcact accaaccgat
    22261 tgccggcagc ttcccggcca agtacaaaac gcgctttgac ggctatgccg atcaggtgct
    22321 gccggcgatc caggagcccc tgctcagcca gcacgaaggc ctggtattcg ccatcgcctg
    22381 taccgccgaa ggctatgtgc cgacgcacaa caacgcgttc aaccatgccg ccaccggcga
    22441 ccgcgccgtg gacaccctga agagccgcag caagcgcctg ttcaacgacc gcaccggcat
    22501 ccgctgtggc agccatcaga agccgttgct gctacagacc tatatgcgcg acaccggcga
    22561 gctgatgcat gacctctcgg tgccgattat ggtcaacggc cggcattggg gcggtttgcg
    22621 tctgggttac aagccggagc cctgagcgca ggcaacgcct ccgccagcag ccgaagcgtt
    22681 gccgcccaca gttcagggcg ctcgacccga cgcaacaatg ttttgaatta tcgagtctgg
    22741 ctggggttct taagctgtag gccatttatc ggccatccgc ttccaaggac accctatgaa
    22801 gatcctgctg gtactgacct cccacaacca actgggcgac accgggcaca agaccggttt
    22861 ctggctggaa gagttcgcct cgccctatta cgtattcaag gatgccgggg cgcagctgac
    22921 cctggcctcg cccaagggcg gccagccgcc gctcgacccg aaaagtgacg tcgcagacgc
    22981 gcaaacgccg accaccgaac gcttcaaagc cgatgttgcc gcgcaacagg cgctggccaa
    23041 taccctgccg ttaagccagg tgcaggccga tgacttcgac gccgtgttct atcccggtgg
    23101 ccatggcccg ctgtgggatc tggccgaaga ccgcacctcc attgccctga tcgaacgctt
    23161 ccaggcgcag aacaaacctg tagcggcggt ctgccacgcc ccgggtgtgc tgcgccatgt
    23221 gaaggcggcc aacggcctgc ccctggttag cggcaaaaag gtcaccggtt tcagcaacag
    23281 cgaagaagca gctgtgcaat tgaccgaagt ggtgccgttt ctggtgcaag acatgcttat
    23341 caccaacggc gggcactact ccagcgcgaa cgactggcag agccatgtgc tggtcgatgg
    23401 gctgctgatc accgggcaga atccatcctc atcggatgcc accgccgagg cgctgatcaa
    23461 gctgctgaaa agccgctaag cagcccgcat ctgccgcaac acaagccgtt ccatgaagaa
    23521 agcggcctga tctggatgaa gaccgggcat cttcccgact agctgccagc cgccatcagc
    23581 cgcttgtact ggcgggtgcg ccgcgcggtt ttcagttgtt ccagcagcag tgcccgtagc
    23641 ggcgaaaccc cggcgtgggt gtgcttgccc aggctgagct gacgcagctg gaacttcacc
    23701 accgccatat aggccaggga tgccaatgcc ttgtgcttcc agcggatcgg tgccaattcg
    23761 gcgctgctcg actgcccggc ctgccactgc ttgggcagcg ccgggttaat cgccaacgcc
    23821 gtggcgatgc ctgccatggc cacgccgctg gccagtacct gctcgaccac cggcaagcgc
    23881 cgaataccgc cggtgaccat caccggcatc gttgccgctg cggcgatctc accggcaaac
    23941 tcgagaaagt acgcctcacg cgccagggtt cggccgtcgc gggcatcgcc ctgcatggcc
    24001 ggggcttcgt agctgccgcc ggacagttcg accagatcaa catccaactg gttaagcagc
    24061 tgcaccacgc gcttggcgtc ggctgcatcg aaaccgccgc gctggaagtc cgccgagttg
    24121 agttttaccg ccacgcagaa gctcggcgcc accaaggcgc gcaccgcctt gaccacttcc
    24181 agcagcaggc gcgcacggtt ttccaacgaa ccgccccagc tatcagtgcg cttgttgctg
    24241 atgggcgaaa ggaactggct aagcaggtaa ccgtgcgccg cgtggatctg cacgccgcta
    24301 aagccagcct gttcggccaa ctgcgcggtg cgcgcgaaac gctgaatcag ctcatggatt
    24361 tccgcttcat ccagcgcctt gggcacgggg aacatcttcg acagaccgcc cagctccagg
    24421 gccacggccg agggcgcgac ggtctgctgg ccgaggttgg cctgcatctg ccggccgggg
    24481 tggttgatct gcatccagaa ctgcgcgccc tgggcacgac cgatgcgcgc ccactcgcgg
    24541 aatttttcca gctggcgttc atcttccagc actacgccgc cggggccggt catggcacga
    24601 cggtcgacca tcacgttacc ggtgagcaac aacccagcac cgccctcggc ccaggcctga
    24661 tacaactgca acagttcggc ggacgggcct tggctggcgt cggcgaggtt ctcttccatc
    24721 gccgccttgg cgatgcgatt gaacacggtg gtgccgttgg gcagttgcag cggttggaag
    24781 gcagtcatgg cgtactccgg ggtagcagat ggagcaagcc taaggttaaa gtcaacttta
    24841 aggtcaatag cccggagcat agattatgaa aattggtgaa ctggccaccc gcagcggtct
    24901 ggccgcctcg cgcatccgct tctacgaggc cagcggactg atcaacgccc agcgtcaggc
    24961 caacggctat cgcagctata cggagcagac actgcagacc ctcggcatta tcagctgcgc
    25021 ccagcaggcc ggtttcagcc tggaagaaat ccgccgtctg ctgccagacg ccaaccccca
    25081 gggctgggcg catgagcaga tgctcggcag cctgcaacgc aaggtcgtcg agatagaggc
    25141 gatgcagcaa cgtctggcgc agaacaaggc gcagctgctg accatcatcg ccaccatcga
    25201 gagcaaacca gaaggcatgc cctgcacgga aaacgccgag cgagtgctgg ccaatctgcg
    25261 cggcagttga ctcaggccga cagcggccag gttcaatgtt tagccaggca ataaccaagc
    25321 atgtgaaaaa caggatttaa cgcactcttg gccctgtgtg aacctgctgg ccatgtgttc
    25381 ttccgtacag ctgttcctca cccccctatt ggctgcgcat cggtgcgagc ggcgcagctt
    25441 tgtgcccccc gccctgctgc cacagatcct cgccttctgg gccctgtggc actgccgcca
    25501 cgcgcgcccg ccggatgccc tgagcacaga tcactgatcg cctcgcagcc agcaatcctg
    25561 gctgccgacc ctgtgcccgg ttcgggccca gcacccgcca gtcgtaccca ggaaccgccc
    25621 tatgaacgcc gccatcaaac ccgtaacccc cgccgccgag cgctgccctt cctactacag
    25681 cgccagcctc aatgaggaaa ccgactaccc gaccctgcag ggcgaggtca gcgtcgatgt
    25741 ggtgatcatc ggtggcggct ttaccggcgt ggcgtccgcc gtggagctgg ccgagcgcgg
    25801 cctcaaggtg gccatagtcg aagccaacaa gatcggctgg ggtgccaccg ggcgcaatgg
    25861 aggccaggtc accggcagcc tgtcgggcga tgcggcgatg aacaaacaga tgcgcaagac
    25921 cctcggcgag gaggttgaag actttatctg gcacctgcgc tggcgcggcc acgagatcat
    25981 caagcagcgg gtggagaagt acggtatcca gtgcgacctc aagcacggcc acctgcacgc
    26041 ggcgatgaag ccggtgcata tgcaggaact gcaggccacc tacgccgaag cgcaacgccg
    26101 tggcatgggc gctgacgtga ccctgctcga ccgcgccggc gtgcagagcc atctggccag
    26161 cgacctgtac agcggcgcgc taaagaacag ccgcaatatg cacctgcatc cgctgaacct
    26221 gtgcatcggc gaagccaagg ccgccgccag cctgggcgcg ctgattttcg aacactccga
    26281 agtgctggaa attatccacg gcgacaagcc ggcggtggtt accgcagcgg gccggatcaa
    26341 cgccaagcag gtgctgctag ccggcgacgt gtaccacaag ctggagccga aaaagctcaa
    26401 gggcatgatc ttcccggcca tgggcggcat agtcaccacc gcgccgctgg gtgagctggc
    26461 gaaggaaatc aacccgcagg acctggccgt gtacgactgc cgcttcgtcc tcgactacta
    26521 ccgcatgacc gccgacggcc gcctgctgtt cggcggtggc tgcaactact ccgggcgcga
    26581 ctcgcgggat atcgccgccg agctgcgccc cggtatcgag cagaccttcc cgcagctcaa
    26641 gggcgtcgct atcgacttcc agtggagctg cgccatgggc atcgtgatca accgcatccc
    26701 gcagctgggt aagctctcgg acaacgtctg gtactgccag ggctactccg gccacggcat
    26761 cgccaccact cacatcatgg gcgagatcat ggccaacgcc ctgaccggca ccctcggcca
    26821 ctacgacacc ttcgccgcct gctcacagat caaggtgccg ctgggcgacc tgttcggcaa
    26881 cccgatgctg gcagcgggca tgtggtacta ccagatgttg gaaaaactgc gttaagccat
    26941 tcgccataac agatcgcggg catggcggag tgccgcccag ccgctcctac agggttacgc
    27001 atatcgtagg agcgggccat gcccgcgagc ctttagcctt tgccaacaag gctgtgcgcg
    27061 acctgcgggg ccggcgcctc atcacgatca tcgacaaagg ccaccgccgg cgagccatcg
    27121 gcattcagct ggtagatcgc gctcggctgg ccttgctcat tgaagaacac ctggcccagc
    27181 cccgcgccat tccacagacg ctcgccggcc ttgctgcgcg aggggatgcg cgggctgacc
    27241 tgcatgcgcc ggcgcgtggt cagccagttg agcggcgacc agggtgcata taaccagcgg
    27301 ttgaggcggt cgaaccagtc gagcaggcgc ggtgggaatt cgtttttcaa gccgctgctg
    27361 gtgatctgcc agagcttcgg cccgcgcttg cgttggcgaa tcagcacctc gtagacaaac
    27421 gagtaatgca cgtcgccgga gagaatcacg tagtttcccg gcgtgcgcga gtggcggaag
    27481 atattcagca tcacctgcgc tgcgccacgg tgggccatcc agttttccgc atcgaccagc
    27541 agtgggtggc cggcccagct gaagaatttc tgaatggtct cgatcagctt gacgccgaac
    27601 atcggtgcag gcgaaacgat gatcgccgcc ggatgatcaa gcagctcctg ctgaaactcg
    27661 cacagcgctt cccaatccag caggccggag ggccgcttga ggttgcgctc gctgcgccaa
    27721 cgcctggtgc gggtatccag caccaccagc gccgggctgc tcggcagcac gaactgccac
    27781 tggtgaaagc tcagcagctc atcaatcagc gcgtcctgcg gctgactgtt cataaggttg
    27841 gcggcatcgc gcccctcggt catcgcgcgg atcatctgca gcggtttggc gaacacatcc
    27901 gggttgttac cccagccctg gcacagcagg taggcgatca gcgcattgcc gatgatgcgt
    27961 ttggaaaacg ggtggccata ggcggtttcc tcccactgcg cggagaggtt ccagtcatcg
    28021 gtgatgtcgt ggtcatcgaa gatcatcagc gtcggcaggt gggcgagggc acgggcgacc
    28081 tggcccaggc cgccgcgaaa ggcttcgatg tgcccacgct cggcctgata acgcggcact
    28141 tcctcggcgc tgagtgccgg catttcggca tccagcaacg tccagggcac gggcgaccag
    28201 accagcaggt acatggccag cacttcggcg caggtcacca ggtggttgtc ggcgctgctg
    28261 gtggtgaata ccggcttctc cacgccgccg aaaaaccgct cgcgcagggt ttcgttgctc
    28321 ttcagcgccg gcagcaactc ggcgcgttgg tagtagctgg ccgggtgctg gtacagcgcc
    28381 gtgctgtcat cgaccaccgc accttccagg tgctcgccaa acaaacccag gcgctcgatc
    28441 acctgatgaa tcgcccgcag cagcggcccg ctgacgtcgt cggcgtagat ctggtcgcca
    28501 ctcatcagca gcaaccctgg acgctgctcg gggtcgtgcg gctcggccag caggcgatcg
    28561 gcgcagagca ggccatcggc cgccgggtgg tgcggtttgc ggcaggagcc atgcagcaac
    28621 tggtcgaggc gcgcacgcag aacgaaattg gcctgggtcg cgccgtcata cagcaggtgc
    28681 ggtgcccagt cggcgatgcc caacgcggcc tggccaggct gctcgatcag caggtcgtaa
    28741 tcaatctgca catcgcaggg cagcgccgcg tcgagcgcca gatcgatcag gtggataaac
    28801 gcctgctcgc cgatctggat gacctggcag ctgtcgccct gcagcgggta gctctgtggc
    28861 tctggcgcgt cgccatagct cagctgcagg ctaaacgaca acggctggct gccgaccagc
    28921 cagagcacca gacgctgcgg ctccatgcgg cgcagcagcg ggccggccag caccaggggc
    28981 aggtcggcgt gggtaacagg ggcgggtatc ggcatccgga cagcagctct agtgaaggta
    29041 ggggtccatc atagagcgcc aggtggcctc ggagattccc cgcgggcagg ccgccatgta
    29101 gcaaaacgtt actgcgaggc ctggcgcagg cgcggcagat cgaggatttc gatctcgcca
    29161 taggtcaggc gcacaatgcc ctggccttcc agttccttga ggatctggtt ggtggtctgc
    29221 cgggagatcg ccagcatcag cgccagctgt tcctggggca gatggatgac ccggcgcggt
    29281 tcgccttcgc cgtagttctc ggcaatcagc agcagccgcc gagccaggcg cggggcggcg
    29341 ggcagcaggc tcatctcttc cagggcgata aacgccaggc gcagcttgtg gctcatcagc
    29401 agggcgaagt cgcgccagta ttgcggctgc tgttcgagca gcgtcagcag ccgcgcctgc
    29461 ggcactagca gcagagtggt agcgccttcg gcaaaggcat cgtgggtgcg cggctggcca
    29521 tcgaacaggg aaatctcgcc aaaccagtag ggcggctcga ccagggtcaa caacgcctcc
    29581 ttgccctgcg cactcactgc gccgatgcgc atgccgccct cgaccaccgc atacaggcca
    29641 cagggtttgt cgccacggcg aaatagcacc tgaccgggtg ccagctgctg cacctgagca
    29701 gtggccaaca gcgcttcctg caaggcggcg ggcagggcgc tgaaccagtg gccttggcac
    29761 aagcgcgcct gatagctgcg gacgctgttc atcggcggtt ccaagaggtt tgtcggctag
    29821 ctgacagagc gctgcgcggg cgtgagttca tgctggccac ctgtcttgca tgaggaaaat
    29881 aataatgaaa accctggttg atcatctggc tcaatacgcg gcctatcacc gcgaccaacg
    29941 caatattctc agccacttta tcggtattcc actgatcg
//
LOCUS       DASRUM010000315         3008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1998541, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000315 DASRUM010000000
VERSION     DASRUM010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1998541"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..906)
                     /locus_tag="VFF22_21170"
     CDS             complement(118..906)
                     /locus_tag="VFF22_21170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020299273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HZX19365.1"
                     /translation="MEHKDSVQDDLESTLKHNARQLVDSLEQGNFGEAVQLIHELNKA
                     RDRGLYHEVGKLTRELHNAIVNFQLDPRVPHATEVSQITDATERLNYVVTMTEKAANR
                     TMDLVEQSAPLVNDLSDEAQSLSADWGRFMRREMSADGFRELAKRVELFLARSERDGA
                     KISGHLNDILLAQDYQDLTGQVIKRVTQLVTEVESNLLKLVLMASQVDQFAGIEHNHE
                     LLRAEQEKQKEPSHGEGPQIHADKREDVASSQDDVDDLLSSLGF"
     gene            complement(926..1300)
                     /locus_tag="VFF22_21175"
     CDS             complement(926..1300)
                     /locus_tag="VFF22_21175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HZX19366.1"
                     /translation="MKILIVDDFSTMRRIIKNLLRDLGFTNTAEADDGVTALPMLQSG
                     SFDFLVTDWNMPGMTGIDLLRAVRADERLKTLPVLMVTAEAKRDQIIEAAQAGVNGYV
                     VKPFTAQVLKEKIEKIFERVNG"
     gene            complement(1398..2144)
                     /gene="fliA"
                     /locus_tag="VFF22_21180"
     CDS             complement(1398..2144)
                     /gene="fliA"
                     /locus_tag="VFF22_21180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HZX19367.1"
                     /translation="MTAASGLRMYSKAQAQDSQHQLIERYAPLVKRIAYHLLGRLPAN
                     VQVDDLIQAGMIGLLEASKKYDSSKGASFETFAGIRIRGSMLDEVRKGDWAPRSVHRN
                     SRMVSDAIRKIEARTGRDAKDQEVAAELQLSLEDYYGILGDTLGSRLFSFDDLLQDGE
                     HGGLGEDAGSNHLEPSRDLEDERFQAALADAIANLPERERLVLALYYDEELNLKEIGE
                     VLGVSESRVSQLHSQCAARLRARLGEWRAH"
     gene            complement(2141..2971)
                     /gene="fleN"
                     /locus_tag="VFF22_21185"
     CDS             complement(2141..2971)
                     /gene="fleN"
                     /locus_tag="VFF22_21185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar synthesis regulator FleN"
                     /protein_id="HZX19368.1"
                     /translation="MGMHPVQVIAVTGGKGGVGKTNVSVNLALALADLGRRVMLMDAD
                     LGLANVDVLLGLSAKRTLEDVISGECELRDVLLQGPGGIRIVPAASGIQSMVQLSPMQ
                     HAGLIQAFSDISDNLDVLIIDTAAGIGDGVVSFVRAAQEVLVVVCDEPTSITDAYALI
                     KLLNRDHGMNRFRVLANMAHSPQEGRNLFAKLTKVTDRFLDVALQYVGAVPYDESVRK
                     AVQKQRAVYEAFPRSKCALAFKAIAQKVDAWPLPANPRGHLEFFVERLVQQPTADTAI
                     "
BASE COUNT          639 a          908 c          787 g          674 t
ORIGIN      
        1 ccgggcggct ttccagctct accagttgct cggacagtag ttcaagaatc tcgccggcct
       61 ctaccaggaa gtcctggagg atttcttcat cggcgccgaa gctcataaga cgtgctccta
      121 aaatcccagg ctggaaagca gatcgtcgac atcatcctga ctggatgcga cgtcttcacg
      181 cttatcggca tgaatctgcg gaccttcacc gtgcgaaggc tctttttgtt tttcttgctc
      241 ggcgcgaagc aactcatggt tgtgctcaat gccggcaaac tgatccacct ggctggccat
      301 cagcaccagt ttcagaaggt tgctttccac ttcggtaacc aactgggtta cgcgcttgat
      361 cacctgcccg gtcaggtctt ggtagtcctg agccaacaaa atgtcattga gatgcccaga
      421 aatcttggca ccatcacgct cgctgcgagc gagaaacagc tcgacgcgct tggccagctc
      481 acgaaaacca tcggcgctca tctcgcggcg cataaagcgc ccccagtcgg cactgaggct
      541 ctgcgcttca tcactgagat cgttaaccag tggggcgctc tgctcaacca gatccatggt
      601 gcgattggcc gctttctcgg tcatcgtcac cacatagttc agccgttcgg tggcatcggt
      661 gatttgcgag acctcagtgg catgcggcac acgggggtcg agctgaaagt tgacgatggc
      721 attgtgcagt tcacgggtca gcttgccgac ctcgtgatac aggccgcgat cacgcgcctt
      781 gttgagctca tgtatcagct gtaccgcttc accaaagttg ccttgctcca ggctgtcgac
      841 caattggcga gcgttgtgtt tcagggtcga ctctaggtca tcctgaacgg aatctttatg
      901 ttccatagca ccctcatggc agacatcagc cgttgactcg ctcgaagatt ttctcaatct
      961 tctctttcag tacctgggca gtgaacggct tgacgacata accgtttacc ccagcctggg
     1021 ctgcctcgat gatctgatcg cgcttggctt cagcggtaac catcaacacc ggtagagttt
     1081 tcaggcgctc atcagcacgc accgcacgta gcaggtcgat acccgtcata ccgggcatgt
     1141 tccagtcagt caccagaaaa tcgaaactgc cgctttgcag catgggcagc gcggtcacgc
     1201 catcatcggc ttccgctgtg ttggtgaacc ccaagtcacg caggaggttc ttgatgatcc
     1261 gtcgcatcgt tgaaaagtca tcaacgatga ggattttcat gttcttgtcc aagtcgacct
     1321 ccgtacagtc ccaaacgcac ccagcaacct gtatgcgctt ttaatcgtga aaccggccca
     1381 gcgttagcga atgcaactca atgtgcgcgc cactcaccca gacgagcgcg taaacgagcg
     1441 gcacattgac tgtgtagttg gcttacacgt gattcgctaa cccccaacac ctcgccgatc
     1501 tctttaagat tcaattcttc atcgtaatat agcgctaaca ctaggcgctc gcgctccggc
     1561 aggttggcaa tggcatccgc cagggccgcc tgaaagcgtt catcttccag atcacgagaa
     1621 ggttcgaggt ggttgctgcc agcgtcttca cccagcccac cgtgttcacc gtcctgcaac
     1681 aggtcgtcga aactgaacag gcggctgcct aaagtgtcgc caagaatgcc gtagtaatcc
     1741 tcgagactca attggagttc ggccgcaacc tcttgatctt tagcgtctct gcccgttctg
     1801 gcctcaattt ttctaatcgc gtcgctgacc atgcggctat tgcgatgcac cgaacgtggc
     1861 gcccagtcac ctttgcgcac ctcatcgagc atggagccac ggatacgaat accagcgaat
     1921 gtctcgaaac tggcgccttt actggagtcg tatttttttg acgcttcgag caggccaatc
     1981 atcccggcct ggatcaagtc gtcgacctgc acattggccg gcagacggcc cagcaggtga
     2041 taggcaatgc gtttgaccag aggcgcatag cgctcgatca attggtgctg ggaatcctgc
     2101 gcctgtgcct tgctatacat acgaagtcca gaggctgctg tcatatggcc gtgtctgcgg
     2161 tcggttgctg caccagacgc tcaacgaaaa actccaaatg accgcgcggg ttggccggta
     2221 agggccaggc gtcgactttc tgggctatcg ccttgaacgc cagcgcgcac ttcgagcgcg
     2281 ggaaggcttc atagaccgcg cgctgcttct gcacggcctt gcggactgac tcgtcgtacg
     2341 gcactgcacc tacatactgc agggcaacgt cgagaaaacg gtcagtaact ttagtcagct
     2401 tagcaaagag gttgcgtcct tcctgcgggc tgtgggccat attggccaat acacggaagc
     2461 gattcatgcc gtggtcgcgg ttaagcaatt tgatcaatgc gtaggcgtcg gtgatcgacg
     2521 tcggttcatc acacaccacc accagtactt cctgggcggc gcgcacgaag ctgaccacac
     2581 catcaccgat tccggcggca gtgtcgataa tcagcacatc gaggttgtca ctgatatcgc
     2641 tgaaggcctg aatcagcccg gcatgctgca tgggtgacag ctgcaccatg ctctgaatgc
     2701 cagacgccgc cggcacgata cgaatgccgc ccggcccctg cagcagtaca tcgcgcagct
     2761 cacattcgcc gctgatgacg tcttccaggg tgcgtttggc gctcagacca agcaacacat
     2821 cgacgttggc caagcccagg tcggcgtcca taagcatcac ccgacgaccg agatcagcca
     2881 gggccaacgc caggttcacc gagacgttgg tcttaccgac gccacctttg ccgccggtca
     2941 ccgcaatcac ctgtacggga tgcataccca tatttctaca caccttgtct aacttcgact
     3001 ggagccac
//
LOCUS       DASRUM010000316         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_1999276, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000316 DASRUM010000000
VERSION     DASRUM010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1999276"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..108)
                     /locus_tag="VFF22_21190"
     CDS             complement(<1..108)
                     /locus_tag="VFF22_21190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HZX19369.1"
                     /translation="MADVNKVVLAYSGGLDTSVILKWLQDTYNCEVVTFT"
     gene            complement(239..1156)
                     /locus_tag="VFF22_21195"
     CDS             complement(239..1156)
                     /locus_tag="VFF22_21195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017480125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZX19370.1"
                     /translation="MRPYSLAVLCLLVSQSVLAISFQTRLEKIEWKVEGDQFECRLSQ
                     PITDFGSGMFVRRAGEQVTFSLKARERWLGAGNATLLAAAAPWQPGRGDINLGSVSVV
                     SAEVLFNSSQEQGTRLLTGLLEGRSPVVRHRAAHGGDAVEVRLLPAKFNKAYNDYLDC
                     TAKLLPVNFDQVRKSQVGFPGGGVALEPLAQAKLDIILSFLKADPSVNRIQLDGHADN
                     SGNRLTNRDLSRRRALAVMEYLKASGIPAEQITMRFHGERYPVAPNNNEANRAKNRRV
                     SILLDRVPEAAVPATPAPAPAAADPAATS"
     gene            1362..2408
                     /gene="pyrC"
                     /locus_tag="VFF22_21200"
     CDS             1362..2408
                     /gene="pyrC"
                     /locus_tag="VFF22_21200"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZX19371.1"
                     /translation="MTDRLTLLRPDDWHIHLRDGGVLPHTVADVARTFGRAIIMPNLV
                     PPVRNASEADAYRQRILSARPAGSRFEPLMVLYLTDLTQPEDVRTAKASGFVHAAKLY
                     PAGATTNSDSGVTSIDKIFPVLEAMAEVGLPLLVHGEVTRSEIDVFDREKVFIGEHLS
                     RVVERFPTLKVVFEHITTRDAVEFVQGASANVGATITAHHLLYNRNHMLVGGIRPHFY
                     CLPILKRNTHQDALLDAATSGNAKFFLGTDSAPHAKHAKEAACGCAGCYTAYAAIELY
                     AEAFEQRNALDKLEAFASLNGPDFYGLPRNSDSITLVREDWTAPASLPLGEQTVIPLR
                     AGETLRWRLLESQA"
     gene            2405..>2953
                     /gene="rnt"
                     /locus_tag="VFF22_21205"
     CDS             2405..>2953
                     /gene="rnt"
                     /locus_tag="VFF22_21205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HZX19372.1"
                     /translation="MSEDLYDDEAESGSHSGPRHPMAARFRGYLPVVVDVETGGFNCA
                     TDALLEIAATTIGMDEGGFLYPEHTQFFRIEPFEGANIEQAALDFTGIKLDHPLRMAV
                     TEEHALTEIFRGLRKSLKANSCKRAILVGHNSSFDLGFLNAAVNRTGIKRNPFHPFSS
                     FDTATLAGLAYGQTVLAKACQAA"
BASE COUNT          583 a          984 c          843 g          543 t
ORIGIN      
        1 ggtgaaggtc accacttcgc agttataggt atcttgcagc cacttgagga tcacggaagt
       61 gtccaggcca ccggaatatg ccaggactac cttgtttacg tcggccatgc catcaactcc
      121 acggggttgt gcgggaaaac ccgtgattct actgcccatg ccgcctgatt tacagaggcg
      181 cgacagcttg tgacgaccag cgacggataa ttgttggaca acagacgaat cgcaggcgct
      241 aggaggtggc cgcagggtcc gccgctgcag gcgctggagc aggcgtagcc ggcactgccg
      301 cctcaggcac ccggtcgaga aggatgctta ctcggcgatt tttcgcccga ttggcttcat
      361 tgttatttgg cgccaccggg taacgctcgc catggaaacg catggtgatc tgttcagccg
      421 gaataccact ggccttgagg tactccatca ccgccagcgc gcggcggcgc gacaggtcac
      481 gattggtcag acgattaccg ctgttgtcag catgcccatc aagctggatg cggttgacgc
      541 tgggatcggc cttgagaaag ctgaggatga tatccaactt ggcctgggcc agcggttcca
      601 gcgccacgcc accccccgga aaaccgacct gacttttgcg cacctgatcg aaattcaccg
      661 gcagcagctt ggccgtgcaa tcgagatagt cgttgtaagc cttgttgaat ttagcgggca
      721 gcaagcgcac ttctactgca tcaccgccgt gcgcagcacg gtggcgcacc acggggctgc
      781 gcccctcaag caggccagtc agcaagcgcg tgccctgctc ctgggaactg ttgaacagca
      841 cctcggcaga caccacgctg accgaaccca ggttgatatc gccacgccct ggctgccagg
      901 gcgccgccgc cgccagtaag gttgcattac cggcccccag ccagcgctca cgggctttca
      961 agctaaaggt gacctgttca cccgcccggc gtacaaacat cccggagccg aaatcggtga
     1021 tgggttgcga cagacgacac tcaaactgat cgccttcgac cttccactcg atcttctcca
     1081 gacgagtctg gaagctgatg gctaggacgg actggcttac cagcaggcaa agcaccgcga
     1141 gtgaataggg tcgcacgaca gactccaggg aatacggctt acctgttgtc tatcggtgcc
     1201 acggcggaaa acttgagcag agctttatgc ctcttgtgca ggcagggcca cagtacaacc
     1261 cggccttcag tcaactttac ctgccgcgtc cgctttcagc gtgccgccac tgggcttttc
     1321 cggtagcatt ctcgccacgt tttgcccgcc tggaagcttc catgactgac cgccttaccc
     1381 tgctgcgtcc cgacgactgg cacatccacc tgcgcgatgg tggcgttctg ccgcacaccg
     1441 tcgccgatgt agcccgcacc ttcggccgcg ccatcatcat gcccaacctg gtgccgccgg
     1501 tacgcaatgc cagcgaagcc gatgcctatc gccagcgcat tctcagcgcc cgccccgcag
     1561 gcagccgctt cgagccgctg atggtgctgt atctcaccga cctgacccag cctgaagatg
     1621 tgcgcacggc caaagcctcg ggttttgtgc atgcggccaa gctctacccg gccggcgcaa
     1681 ccaccaactc cgattctggc gtgaccagca tcgacaagat tttcccggtg ctcgaagcca
     1741 tggccgaagt cggcctgccg ctgctggtac acggcgaagt aacgcgcagc gagatcgatg
     1801 tgttcgaccg cgagaaggtc tttatcggtg aacacctgag ccgtgtggtc gagcgtttcc
     1861 cgaccctgaa agtggtgttc gagcacatca ccacacgcga tgcggtggag ttcgtgcaag
     1921 gcgcttcggc caacgtcggc gccaccatca ccgcccacca cctgctgtac aaccgcaacc
     1981 atatgctggt tggcggtatt cgcccgcact tctattgcct gccgatcctc aagcgcaaca
     2041 cccatcagga cgccctgctg gatgcggcca ccagcggcaa tgccaagttc ttcctcggca
     2101 ccgactcggc gccgcacgcc aagcatgcca aggaagccgc ctgcggctgc gccggttgct
     2161 acaccgccta tgcggccatc gagctgtatg ccgaggcctt cgagcagcgt aatgccctgg
     2221 acaagctgga agccttcgcc agcctgaacg gcccggactt ctatggcctg ccgcgtaaca
     2281 gcgacagcat taccctggta cgcgaagact ggaccgcacc ggccagcttg ccgctgggtg
     2341 agcaaaccgt tatcccgctg cgcgctggtg aaaccctgcg ctggcgtctg ctggagagcc
     2401 aagcgtgagt gaagacctgt acgacgacga agctgaaagc ggcagccact ccggcccgcg
     2461 ccaccccatg gccgcgcgct ttcgtggcta cctgccggta gtcgtggacg tggaaaccgg
     2521 tggcttcaac tgcgccaccg atgccctgct ggaaatcgcg gcgaccacca tcggcatgga
     2581 cgaaggcggc ttcctttatc cggagcacac ccagttcttc cgtattgagc cgttcgaagg
     2641 cgcgaatatc gagcaggcgg cgctggactt caccggcatc aagctcgatc acccgctgcg
     2701 catggcagta accgaagagc acgcgctgac ggaaatcttc cgtggcctgc gcaaatcgct
     2761 gaaagccaac agctgcaagc gggcgattct ggtcggccac aacagcagct tcgacctggg
     2821 cttcctcaat gccgctgtaa accgcaccgg catcaaacgc aatccgttcc acccgttctc
     2881 cagcttcgac accgccaccc tggccggcct cgcctatggc cagaccgtac tggccaaggc
     2941 ctgccaggcc gcc
//
LOCUS       DASRUM010000317         8541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_2504651, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000317 DASRUM010000000
VERSION     DASRUM010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 8541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8541
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2504651"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1628
                     /locus_tag="VFF22_21210"
     CDS             <1..1628
                     /locus_tag="VFF22_21210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012724387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HZX19373.1"
                     /translation="PALERLIGFFVNVLPLRARLDSAQDFDARLQRLRDETLAAFQHQ
                     QVPFDKLVETLQPPRQPGVNPLVQALFVLQNTPRSSAELPGLNVEHLAPRQETSKFDI
                     ALFAEEDDQGLTLRWVYRTSLFQRETIEHLQRGFDSLLQQVIEAPENALQRWDWHLAT
                     EPASPPEVSSSDEASRASRKLSKLSKLKQTRATAVSQRPEEQVRTSTLSSDRPLPLVI
                     EPLLGELDPLRWAQQARGWIGEQLRQHGGLLLRGFDLPDASAFEQFAQAIEPDLYGTY
                     GDLPKNNAGKNIYHSTPYPEQHMILFHNESSHLAQWPRKQWFYCETPAAHGGCTPIVD
                     CREVLARLPQPIVETLRSKGLLYVRHFTDKLDVRWQDFFKTGQREEVEQRCRDAGTQW
                     QWLSDDGLRIAQRCPAIVSHPDTGEESFFNQVQLHHPACLEPEVRRNLLELFGPDQLP
                     RNVCYGDGSPIEDEVMAEIGRVYEACAVRFTWQKGDVVMLDNMLVAHARDPFEGERKI
                     CVAMGQMMRRDQLDKSAAQITAHGNVLALEESL"
     gene            1628..5476
                     /locus_tag="VFF22_21215"
     CDS             1628..5476
                     /locus_tag="VFF22_21215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003200893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HZX19374.1"
                     /translation="MEQLHTEAFQLSPQQRQRWQSDLPAATLTLDVRGALDQHALQLH
                     LSGLVQRHEALRLQLCPSPGLRVPLQRIRPEATDLLLDCQALASDHHRLHLQLPALGA
                     DRGTLLRLAAALAAEMPEADSDDEMSYTQYSTWLYEMQAEDDAELGRRFWREQALDEQ
                     SHSELLYREQRSRTATDIRVAQLDGAPELSAMLDDVCRSIHVSPSEVLLTAWCALLQR
                     LSSGDAPVLTMNWVHDCRDDYEELSGCWGLFAKALPLAWRLPQESRFSEALDGLQRMC
                     DRAREWQEYCAIVSDQAPSISRYAFEWGGVMPHALPLANSPGSRLTVLELNAPTQAFE
                     LQLTPEVFTGENGATHYRFNLSYDASSYGMQAVQVLLEQYQSLLLAALADPQQPLAVL
                     PLYSPSFDAALNAVQGKPPHLPAEPFTAFPLRFSQQAARFGERLALCDTTSQLTYAEL
                     ESRSNQLAHYLNAHGIGAEHRVALHLDRNAGAIVAMLGVLKCGAAFLPLDVHQPVARS
                     LAILDAARPAFLLYDGSGTPPALGELNSLDLGDSARWQSQPHSPLSLDIPARSAAYVL
                     HTSGSTGTPKGVVIEHGQLSSYLDSLLGRIAFEPGERSALITSLAADLSYSLLFPSLL
                     TGGELHVVDKATSLDTAAWVAYQTRHLIDHLKTVPSLLDAWLGHSAAAATLPRRNLIL
                     GGENSPQRLLQSLRTLAPTLSVHNHYGPTETTIGVLMHRLDPNVDYRRLPLSDRLDGM
                     RTYLLDEHDQPATAGQAGELYLAGPQLARGYLDKQQTHERFLDNPLQPGERLYRTGDR
                     ARYRPDGSLEIIGRLDRQVKIRGFRVELDEVETQLARLPGIAQVAVTCVTRGPFDQQL
                     FAFLTLANGHGSNLAQLQSQAQERLPEHMLPTLRLVEALPLLGNGKLDRKTLAQWAEQ
                     VLEHVGDSLPRTPLEQLLADIWAGVLGLERVGIEDDFFDLGGHSLAAVKLASRLQNAL
                     GAPVPVNAVFNAPSVASFAELVKAQLQLSPLVQLASAETAVGNLFCFHPSTGHVQDYR
                     ALSEPLQHWNLWGLQASYLVEGSPTPGRDTLDSLAALYVEQLRRQQPEGPYHLLGWSL
                     GGLLAFSAAAQLERAGQQVAFLGIVDSQDQRSAPLETLETLLHSAAQLLDADSQARLQ
                     TLIGAARERLREIPPDQQPLGLVQWARQQGLQLANDSWEQLEYRLRQHGHTQTLIGTF
                     RPVPLRCPIHLWWASATLQAEDFSAPDWSRLTQERTTEQIIEAQHLDILQQPALQQAL
                     QEHLKALGNTPHERLNNEAS"
     gene            5478..7115
                     /locus_tag="VFF22_21220"
     CDS             5478..7115
                     /locus_tag="VFF22_21220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic peptide export ABC transporter"
                     /protein_id="HZX19375.1"
                     /translation="MDLLLLLARRSWPTLLIATATGLVCGLAAAGLIATINHSLAGFS
                     QLRPADGLLFAGLVALVVASRIVSDVSLLRLGQTAVSDMRLHLSGKLIDTPYPRLQRL
                     GKHRLLAMLTDDTQTLSQAVELVPIMLVNSGIVLACLGYLGWLSLPLLGLTVALILLG
                     AASFHWPQRKALTSISKARELKDQLFDQYRLLTDGSKELQLNHGRRLQFFERLLYPIS
                     QQYRRDFVRGMSIYALVLNWGNGIFYLLIGMVLFIAPQFIDLGATLVTGYILTLLYMI
                     TPLSELMNALPTLGKASVSLRKIRALEGQLEVAATSGDASAPASSVQELSCREVRHTY
                     YREGEDGSFTLGPFDLTLKAGELIFITGGNGSGKTTLALLLTGLYQPEGGDLQLDGGV
                     SSSTQNHLYRQHFSAIFTDFCLFEHLLDGDDPQLMQQARDYLAHLQLEHKVSIEGGRL
                     STLDLSTGQRKRLALLAAYLDDRPCYLFDEWAADQDPVFKRFFYTKILPDLRQRGKLV
                     IVISHDDAYFSLADRLIKVDQGAVHDTRPEPLAAAAY"
     gene            7128..7895
                     /locus_tag="VFF22_21225"
     CDS             7128..7895
                     /locus_tag="VFF22_21225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZX19376.1"
                     /translation="MHAQLSLNRPSMQDSPWIHTRALPSARLRLFCLPFAGGSASSFM
                     PWFATLAPDIQLCAIQLPGRGARFGEPAIHDFAELIEQLGCVIAQHDDLPYAFFGHSL
                     GALVAFELSHHCVRKGLELPRHLFVSASGAPRHRVGAARYDLLSDRELLAKLRGYAGT
                     PPEVLANQELMELLLPAIRADFALLRHYRYQPRPRLGIPLCVLAGRDDQHVASETLTD
                     WQGETSATCEQHRFDGGHFFAFEQADSVLELVKRKLS"
     gene            complement(7892..>8541)
                     /gene="fhuF"
                     /locus_tag="VFF22_21230"
     CDS             complement(7892..>8541)
                     /gene="fhuF"
                     /locus_tag="VFF22_21230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008051971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0033214 - siderophore-dependent iron import
                     into cell [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HZX19377.1"
                     /translation="RFAELYPGIKRPPVASQWSMNYLSVLLPATLAAVLTRDMTIAFW
                     DQDVTLLHDAGQPMALKVPHELAVLAAEQRAEYWSRLMQEHLEPLFKTLALWGGLAPK
                     VLWGNVVGVWDGAFSRLDGSLTREGFAEAHRWLEQAVVADGRLKLRSLQWMVESPAPD
                     VCASIPLRRHCCLHYQLHAPVAGKPQVLCESCPKLHRLPQAEQASYLRSIYLGES"
BASE COUNT         1578 a         2912 c         2570 g         1481 t
ORIGIN      
        1 atccagcgct ggagcggctt attggcttct tcgtcaacgt gctgccgctg cgcgcgcgcc
       61 tcgactcggc gcaggacttc gacgcccgcc tgcagcgcct gcgcgacgaa accctggctg
      121 ccttccagca ccagcaggtg ccgttcgaca agctggtgga aaccctgcag ccgccacgcc
      181 agcctggggt caacccgctg gtgcaggcgc tgttcgtgct gcagaacacc ccccgttcct
      241 ccgccgagct gccgggcctg aacgtggagc acctcgctcc gcgccaggag accagcaagt
      301 tcgacatcgc gctgttcgcc gaggaggacg accagggcct gaccctgcgc tgggtctacc
      361 gcaccagcct gttccagcgc gaaaccatcg agcacctgca acgcggtttc gacagcctgc
      421 tacaacaggt tattgaagcg cctgaaaacg ccttgcaacg ctgggactgg caccttgcta
      481 cagagccagc cagcccgcct gaggtttcct ccagcgacga ggctagtcgt gcctcgcgca
      541 agctgtccaa actgagcaag ctcaagcaaa cccgcgccac cgccgtcagt cagcggcccg
      601 aggagcaggt acgcacctcg actctctcca gcgaccggcc cctgccgctg gtgattgaac
      661 cgcttctggg cgagttggac ccgctgcgtt gggcgcaaca ggcccgcggc tggatcggcg
      721 agcagttgcg ccagcatggt ggcctgctgc tgcgcggctt cgacctgccg gacgccagtg
      781 ctttcgagca gttcgcccag gccatcgaac ccgacctgta cggcacctac ggcgatctgc
      841 cgaagaacaa cgccggcaag aatatctacc actccacccc ttacccggag cagcacatga
      901 tcctgttcca taacgagagc tcgcatctcg cgcagtggcc gcgcaagcag tggttctact
      961 gcgaaacgcc cgccgcgcat ggcggctgca cccccatcgt cgattgccgc gaggtgctcg
     1021 cgcgcttgcc acagccgatc gtcgagacgc tgcgcagcaa aggcctgctc tacgtgcgcc
     1081 acttcaccga caagctcgat gtgcgctggc aggacttctt caagaccggg cagcgcgaag
     1141 aagtcgaaca gcgctgccgc gacgccggca cccagtggca atggctgagt gacgatggcc
     1201 tgcgtatcgc ccaacgctgc ccggccatcg tcagccaccc ggataccggc gaggagtcgt
     1261 tcttcaacca ggtgcagttg caccaccccg cctgcctgga accggaggtg cggcgcaacc
     1321 tgctggagct gttcggccct gatcagttgc cccgcaacgt ctgctacggc gacggcagcc
     1381 ccatcgagga cgaggtgatg gccgagatcg gccgcgtcta cgaggcctgc gcggtgcggt
     1441 tcacctggca gaaaggcgac gtggtaatgc tcgacaacat gctcgtcgcc cacgcccgcg
     1501 accccttcga gggcgaacgc aagatctgcg tcgccatggg ccagatgatg cggcgcgacc
     1561 aactggacaa gagcgcggca caaatcaccg cgcacggtaa cgttctggcc ctggaggaaa
     1621 gcctgtgatg gagcaactgc ataccgaggc cttccagctc tcgccccaac aacgacagcg
     1681 ttggcagtcc gacctgccgg ccgccacgct aaccctggac gttcgcggag cgctggatca
     1741 gcacgccctg cagctgcacc taagcggcct ggtgcaacgc catgaggcgc tgcgtctgca
     1801 gctatgccca tcgcccggct tgcgtgtgcc tctacagcgc atccgcccgg aagcgaccga
     1861 cctgttgctg gactgtcagg ctctggccag cgatcaccac cgcctgcatc tgcaactccc
     1921 ggcactgggc gccgaccgcg gcacgttgct gcgtctcgcc gcagcgctgg cagcggaaat
     1981 gcctgaggcg gatagcgacg acgaaatgag ctacacccag tacagcacct ggctatacga
     2041 aatgcaggcc gaggatgatg ccgaactggg tcgacgcttc tggcgcgaac aggcattgga
     2101 cgagcagagc cacagcgagc tgctgtaccg cgagcagcgc tcgcgcaccg ctaccgacat
     2161 acgggtcgca caattggacg gcgcgcctga actgagcgcc atgctcgatg acgtctgccg
     2221 aagcatccac gtttccccca gcgaagtact gttgaccgcc tggtgcgcct tgctgcagcg
     2281 cttgagcagc ggcgatgcac ccgtgctgac gatgaactgg gtacacgatt gccgggacga
     2341 ttacgaggag ctgtccggct gctgggggct gttcgccaag gcactaccgc tggcctggcg
     2401 cttgccgcag gaaagccgtt tcagcgaggc gctcgacggc ttgcagcgca tgtgcgaccg
     2461 cgcacgagaa tggcaggaat actgcgccat cgtcagtgac caggcaccca gcatctcgcg
     2521 ttacgccttc gaatggggcg gcgtgatgcc ccatgcactg ccgctcgcaa actcacctgg
     2581 ttcgaggctg acagtactgg agctcaatgc ccctacccag gccttcgagc tgcagctgac
     2641 cccggaagtc ttcacgggcg agaacggggc cacgcactac cgtttcaacc tcagctacga
     2701 cgctagtagc tacggcatgc aagcggtgca ggtgttgctc gaacagtacc agtcgctgct
     2761 gctcgccgcc ctcgccgatc cgcaacagcc gctagctgtg ttgccgctgt acagcccaag
     2821 ctttgacgct gccctgaacg ccgtacaggg caagccgccc cacttaccag ccgagccctt
     2881 caccgccttt cccctacgtt ttagccaaca ggcagcgcgc tttggcgaac gtctggcgtt
     2941 gtgcgatacg accagccagc tgacctatgc cgagctggaa agccgcagca accagttggc
     3001 tcactacctc aatgcccacg gcatcggtgc cgagcaccgg gtcgccttgc acctcgaccg
     3061 caacgccggg gctatcgtcg ccatgctcgg cgtgctcaag tgcggagccg ccttcctgcc
     3121 gctggacgtg catcagccgg tggcccgcag cctggcgatc ctggacgctg ctcgcccggc
     3181 tttcctgctc tacgatggca gcggcacacc gcccgcgctc ggcgaattga acagcctcga
     3241 cctgggcgac agcgcacgct ggcagagcca gccgcacagc ccgctatccc tggatatccc
     3301 cgcgcgcagc gccgcctacg tgctgcacac ctctggtagc acgggaaccc ccaagggcgt
     3361 ggtgatcgag catgggcaac taagcagtta cctggacagc ctgctcgggc gcattgcctt
     3421 cgagccgggc gagcgcagcg cgctgatcac ctccctggcg gcagacctga gctacagcct
     3481 gttgtttccg tcgctgctga ccggtggcga actgcacgtg gtggataagg ctaccagcct
     3541 ggataccgcc gcctgggtgg cctatcagac gcggcacctg atcgaccatc taaagaccgt
     3601 gccctcgctg ctcgatgcct ggctgggaca cagcgctgcc gccgccacac tgccacggcg
     3661 caacctcatt ctgggcggcg agaacagccc gcaacgcctg ttgcagagcc tgcgcacgct
     3721 agcccctacc ctcagcgtgc ataaccacta tgggccgacc gaaaccacca tcggtgtgct
     3781 gatgcacagg ctcgacccca acgtcgacta tcgccgtctg ccgctcagcg accgtctcga
     3841 tggcatgcgt acctacctgc tggacgaaca cgaccaaccg gccacagcag gccaggctgg
     3901 cgaactgtac ctggccggcc cgcaactggc gcggggctac ctcgacaagc agcaaaccca
     3961 cgagcgcttc ctcgacaacc cgctgcaacc cggcgaacgt ctctaccgta cgggcgaccg
     4021 agcgcgctat cgcccggacg gcagcctgga gatcatcggc cgcctcgacc gacaggtgaa
     4081 gatccgcggc tttcgcgtcg aactcgacga ggttgaaacc cagctcgcgc gcttgcctgg
     4141 catagctcaa gtggccgtta cctgcgtaac gcgcggccca ttcgaccagc aattgttcgc
     4201 cttcctcacg ctggccaacg gccacggcag caatcttgcg cagttgcaga gccaggcgca
     4261 ggagcgccta ccggagcata tgctgccgac cctgcggctg gtcgaggccc tgccactgct
     4321 gggcaacggc aaactggatc gcaagaccct ggcgcagtgg gccgaacagg tgctcgagca
     4381 tgtcggcgac agcctgccgc gtacgccgct ggagcaactg ctggccgata tctgggccgg
     4441 cgtactcggc ctggagcggg tcgggatcga ggacgacttc ttcgacctag gcggtcactc
     4501 cctggcggcg gtcaaactgg ccagccgcct gcagaatgca ctcggcgcgc cggtgccggt
     4561 caacgcggtg ttcaatgcgc ccagcgtggc cagcttcgcc gagttggtga aggcgcaatt
     4621 gcagctctca ccactggtgc aactggctag tgccgagacg gccgtaggca acctgttctg
     4681 cttccacccc tcaaccggac acgtgcagga ctatcgcgcc ctgagcgaac cactgcagca
     4741 ttggaatctc tggggactac aggccagcta cctggttgaa ggcagcccca cgccaggccg
     4801 cgacaccctc gattcgttgg cggcgctgta tgtcgagcag ttgcgtcggc aacagccgga
     4861 agggccttat cacctgctgg gctggtcact cggcggcctg ctggccttca gcgccgccgc
     4921 gcaactggag cgcgccggcc aacaggtggc cttcctcggc atcgtcgatt cgcaggacca
     4981 acgcagcgca ccactggaaa ccctggagac gttgctgcac tcggcagcgc agttgcttga
     5041 tgctgacagt caggcccgct tgcagacgct gatcggagcg gcgcgtgaac gtttgcgcga
     5101 aataccgccg gatcaacagc cactcggcct ggtgcagtgg gccaggcagc aggggctgca
     5161 gcttgcaaac gacagctggg agcaattgga ataccgtctg cgccagcatg gccacaccca
     5221 gaccttgatc ggtaccttcc gccccgtccc gctgcgctgc ccgatacatc tgtggtgggc
     5281 cagcgcaacg ttgcaagccg aggacttcag cgcgccagac tggagccgcc tcacacagga
     5341 gcggacgacg gagcagatca tcgaggcgca gcatctggac atcctgcagc aaccggcctt
     5401 gcagcaggcg ctgcaggagc acctgaaggc gctgggcaat accccacatg aacgtttgaa
     5461 caacgaggcg agttgagatg gatctgttac tgctactggc gcggcgctcc tggcccaccc
     5521 tgctgatcgc aaccgccacc ggtctggtct gtggtcttgc cgcagccggc ctgatcgcca
     5581 cgatcaacca ttccctggct ggtttcagcc agctgcgtcc ggcagacggc ctgctgttcg
     5641 ccggcctggt ggcgctggtc gtggcctcgc gcatcgtctc cgacgtcagc ctgttgcgcc
     5701 tggggcagac tgcggtcagc gacatgcgcc tgcacctgag cggcaagctg atcgacacgc
     5761 cctacccgcg tctgcagcgc ctgggcaagc atcgcctgct ggcgatgctc accgacgaca
     5821 ctcaaaccct tagccaagcc gtagagctgg taccgatcat gctggtcaat agcgggatcg
     5881 tactggcttg cctcggctat ctgggttggc tgagcctgcc actgctgggc ctgaccgtag
     5941 cgctgatcct gctcggcgcg gccagcttcc actggccgca gcgcaaggcg ctgacctcca
     6001 tcagcaaggc ccgggagctg aaggatcagc tgttcgacca atatcgcctg ctcaccgacg
     6061 gcagcaagga gttgcaactc aaccacggac ggcgtttgca gttcttcgag cgcctgctgt
     6121 accccatcag ccagcaatac cgtcgggact tcgtgcgtgg catgagcatc tacgccctgg
     6181 tgctgaactg gggcaacggc attttctacc tgctgatcgg tatggtgctg tttattgcgc
     6241 cgcagtttat cgacctgggg gcgacgctgg tcaccggcta catcctcacc ttgctgtaca
     6301 tgatcacgcc gttgtcggaa ttgatgaacg cgctaccgac cctgggcaag gccagcgtct
     6361 cgctgcgcaa gattcgcgcg ctcgaaggcc agctggaagt cgccgccacg tctggcgatg
     6421 cctcagcacc tgcgagcagc gtgcaggaac tgagctgccg tgaggttcgc cacacctact
     6481 accgcgaagg cgaagacggc agcttcaccc tgggcccgtt cgacctgact ctgaaagcgg
     6541 gcgaactgat ctttatcacc ggcggcaatg gcagcggcaa aaccaccctg gcgctgctgc
     6601 tgacggggct ctaccagcct gaaggcggcg acctgcaact ggatggaggt gtttcatcca
     6661 gcacgcagaa ccacctgtat cgccagcatt tttcggcgat ctttaccgac ttctgtctgt
     6721 tcgagcatct gctcgacggc gacgatcccc agttgatgca gcaagcacgt gactatctgg
     6781 cgcacctgca actggagcac aaggtcagca ttgagggcgg tcgtctgtcc acgctcgacc
     6841 tctcgaccgg ccagcgcaaa cgcctggcgt tgctggcagc ctacctagac gaccggccct
     6901 gttacctgtt cgatgagtgg gcggcggatc aggacccggt gttcaagcgc tttttctaca
     6961 ccaagattct tccggatctg cgccagcgtg gaaaactggt gatcgtgatc tcccacgatg
     7021 atgcctattt ttcgctggct gaccggctga tcaaggtgga ccagggcgcc gtacacgaca
     7081 ccaggcccga gcccctcgcg gccgctgcgt attgaggccg ttcaatcatg catgctcagc
     7141 tttcactgaa tcgtccttca atgcaggact cgccctggat tcacacccga gcgctgccca
     7201 gcgctcgcct gcgcctgttc tgcttgccct tcgcgggcgg cagcgccagc agctttatgc
     7261 cctggtttgc cacgctggca cccgacatcc aactttgcgc catccagttg ccggggcgcg
     7321 gcgcgcgttt cggcgagcca gccatccatg acttcgccga gctgatcgaa caactgggtt
     7381 gcgtcatcgc tcagcacgac gacctgcctt atgccttttt tggtcatagc cttggtgccc
     7441 tggtcgcctt tgaactcagc caccactgcg taagaaaggg gctcgagcta ccacgccatc
     7501 tattcgtctc ggccagtgga gcgccccgtc atcgcgtcgg cgcggcgcgc tatgacctgc
     7561 tgagtgaccg ggagctgctg gccaaactgc gcggctatgc cggcacgccg cccgaagtgc
     7621 tggcgaatca ggaactgatg gagttgctgc tgcctgccat ccgcgccgac ttcgcgctgc
     7681 tgcgccacta ccgctaccag ccgcgccctc gtctcggcat tcccctatgt gtactcgccg
     7741 gacgcgacga tcagcatgtg gccagcgaga cactgaccga ctggcagggc gaaacctcgg
     7801 caacctgcga gcaacaccgc tttgacggcg gacatttctt cgcgttcgag caagcggaca
     7861 gcgtgctaga gctggttaag cgcaagttga gctaactttc gccgaggtag atagagcgca
     7921 gatagctcgc ctgctccgcc tgcggtaggc gatgcagctt agggcaggac tcgcacagca
     7981 cctgcggttt tcccgccacc ggggcatgca gctggtagtg caggcagcag tggcgccgca
     8041 gcggaatgct cgcgcaaaca tcgggggcgg gagactcgac catccattgc aggcttcgca
     8101 atttcagtcg cccatctgcc accacggctt gctccaacca gcgatgagcc tcggcaaacc
     8161 cctctctggt gagcgatcca tccagcctgg aaaaggcccc atcccagaca cccacgacat
     8221 tgccccacag caccttgggg gcaagcccgc cccagagagc caatgtctta aacagcggtt
     8281 cgaggtgttc ctgcatcaga cgcgaccaat actccgcgcg ctgctcagcg gcgagaacgg
     8341 cgagctcgtg cggcaccttc aacgccatag gctgcccggc gtcatgtaac aacgtgacgt
     8401 cctgatccca gaaggcaata gtcatatccc gcgtcaacac ggcggccagg gtggcaggca
     8461 gcagaacact gaggtagttc atcgaccatt gggaagcgac cgggggacgt tttataccgg
     8521 ggtagagttc ggcgaagcgc t
//
LOCUS       DASRUM010000318         4532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudomonas sp. isolate SMAG_U8052 k141_829948, whole
            genome shotgun sequence.
ACCESSION   DASRUM010000318 DASRUM010000000
VERSION     DASRUM010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970180
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonas sp. (soil metagenome)
  ORGANISM  Pseudomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:07:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,250
            CDSs (total)                      :: 4,212
            Genes (coding)                    :: 4,145
            CDSs (with protein)               :: 4,145
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 38 of 67
            Pseudo Genes (internal stop)      :: 24 of 67
            Pseudo Genes (multiple problems)  :: 13 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Pseudomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829948"
                     /isolate="SMAG_U8052"
                     /isolation_source="soil"
                     /db_xref="taxon:306"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..1463)
                     /locus_tag="VFF22_21235"
     CDS             complement(276..1463)
                     /locus_tag="VFF22_21235"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZX19378.1"
                     /translation="MPSQAESAVANQHVSAVERLPTGFALRRFEPELETEYRDFSYRS
                     YQQTRRAAICLLLVSFFAFFAFDLLLLYNVGANPITLPVMLVRLCALLIIVFGAYRVL
                     KSPDVATGYRWIPIGLGCLSLGMLSVTLMYHQAQEQMQLPYGLEGMMLIQIVIFVPIG
                     YSYRYSLYLGIATLLCTGLALQLTTSIESCVQYLVSLTYLVAATIAAATAGYCQEHSM
                     RVLFLTKRDLRTMADTDGLTQLYNRRSFDLLLQRALQEARREQTRIALIMLDLDHFKT
                     YNDYYGHPAGDLALQRIAGVLASHARRDLDFAARLGGEEFALVLRNPDSAYLVSTCES
                     IRRNICEELAIEHLKNSAQVMTASLGASFSNTSDTTASLYQRADLALYRAKASGRNRV
                     EID"
     gene            1808..2059
                     /locus_tag="VFF22_21240"
     CDS             1808..2059
                     /locus_tag="VFF22_21240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZX19379.1"
                     /translation="MSQLPEPVLQAMQRGEHDQAIRMLSLNQGITQEAAQEQMNLYLE
                     ENPPIRLRGAGIVGQSRTNALIWLGLIVLMAVAYLLLAN"
     gene            complement(2136..3320)
                     /locus_tag="VFF22_21245"
     CDS             complement(2136..3320)
                     /locus_tag="VFF22_21245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZX19380.1"
                     /translation="MPAVDSSAHLMRGTAAYRRATLVLFCAGFATFALLYCVQPLLPL
                     LAAHFSVSAAGSSLALSLTTLSLAVCLLVSGALAESWGRKPVMAAALGLASLLGIACA
                     LVDSWDHLLWLRALLGLALSGLPALAMAYVGEEFDPEALPAAMGLYIGGTALGGLLGR
                     LLAGLLSDLGGWPLALAGIAGMGLLALGLFVWLLPPSRHFTAQSLSLRGLLRNFALHL
                     GNGELRRLFLLAFLLMGGFVALFNYVGFRLAAAPFNLSSSLIGLLFTVYLLGIVSAGW
                     AGRLVPRLGARKVMQSGIAIMLLGVALCATPWLSAAVAGLALFTLGFFAAHAVASGQV
                     GQRANGAKAQASALYLCAYYLGSSVIGYAAGYIWEHAGWLPMLAVLAALFVAAGVSAR
                     KL"
     gene            3689..4399
                     /locus_tag="VFF22_21250"
     CDS             3689..4399
                     /locus_tag="VFF22_21250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HZX19381.1"
                     /translation="MNTAYLPPCCSPLKSHWPLPHSLPALQLISTTFTPELLGAADFS
                     RCAIEPVRGVAKRQAEYLAGRLCAREALRRLTGIATTPACGEDRAPQWPLGVVGSITH
                     GSGWAGAVVGNSTDWRGLGLDIEQLIPASRAERLAAEILTPAELFRLENLSAEQRAQR
                     ISLTFSLKESLFKALYPQVLQHFYFQDAELLSVDADNKVRMRLLIDLHADWPAGSELE
                     GQFAEFDGYLLSLVSIAR"
BASE COUNT         1096 a         1435 c         1275 g          726 t
ORIGIN      
        1 aagtaaccca aaagaaggcc acccctgcag ccggggccgg atgcaggggt ggccttcttt
       61 tgggttactt tttcttggcc acacaagaaa aagtgactcg cccgtaaggg gcgaaaccaa
      121 aacccaaagt caccacgcta ataaacctgc cgcagaaccc gaacaagaac cgccaacaac
      181 aacaaagctc gcggctaaag cccctcctac atagccaacg tgtagcgctg taaaacctct
      241 cgccactcaa atggcggcac aaacgcatgc cccacttaat cgatttccac acgattacgg
      301 ccacttgcct tggcccggta cagcgccaga tccgcgcgct gataaagact ggccgtcgta
      361 tcactggtat tgctgaagct cgcaccgagg ctggctgtca tcacttgcgc agagttcttc
      421 agatgttcaa tcgccagttc ttcgcagata tttcggcgga tgctttcgca ggtactgacc
      481 aggtaggcgc tgtcagggtt acgcagcacc agggcgaact cctcaccacc cagacgcgcg
      541 gcaaaatcca gatcacggcg cgcatggctg gccaatacgc cagcaatgcg ctgcagggca
      601 agatcgccgg ccggatgacc gtagtaatcg ttgtaggtct tgaaatggtc aagatcgagc
      661 atgatcaacg cgatgcgcgt ttgctcacgc cgggcttcct gaagggcacg ctgtagcaac
      721 agatcaaaac tgcggcggtt gtagagctgg gtcaggccgt cggtatcggc catggttcgc
      781 agatcgcgct tggttagaaa cagcacgcgc attgagtgct cctggcagta accggccgtt
      841 gcggcggcaa tggtcgccgc caccaggtag gtcagcgaga ccaggtactg gacgcatgac
      901 tcgatcgagg tggtcagctg taatgccagg ccggtacata acagcgtagc gatgcccaag
      961 tagaggctgt agcggtagct gtaaccgatg ggcacgaaga tcactatctg gatcaacatc
     1021 atgccctcca ggccataggg cagctgcatc tgctcctggg cctggtgata catcagcgtc
     1081 accgacaaca tgcccaggct aaggcagcca agcccgatag gtatccagcg gtagccagtg
     1141 gcaacgtccg gagacttcaa tactcggtag gcaccaaaaa caataatcag caacgcacac
     1201 aggcgcacca gcatgaccgg cagggttatc gggtttgcgc caacgttgta gagcagcagc
     1261 agatcaaagg cgaagaaggc aaaaaagcta accagtagta agcagatggc cgcgcgccgg
     1321 gtttgctgat aactgcgata agagaaatca cggtattcgg tctcaagttc aggctcgaag
     1381 cggcgtaagg caaaacctgt tggtaaccgc tctacagcgg agacgtgttg attagcaacg
     1441 gctgattcag cttgagacgg catagctgac ctcgcagcga gccttgttgg ctggcctatg
     1501 gcgaaggttt aagggagtgt cactaaaact gacaatagcc gaccagtgcg agtatcaggg
     1561 caattcatct gtgctcttct ggcatcaagc atccacactg ggcttgcctc agttcgtagg
     1621 atgggtcggg ccgcgcaggt tgagcaacgc gatacccatc aaattgccgc agaacctaaa
     1681 cagaaagcag caaaagctcg cggctaaagc cccctctaca aaggcggcgt tatgccttca
     1741 gaagaaaagt atcgaaccct gaacgatggc cgccctctaa aactatctct attggaggaa
     1801 atgtcttatg agccaattac ccgagcccgt cttgcaggcc atgcaacgcg gcgagcacga
     1861 ccaggccatt cgcatgctca gcctgaacca gggcatcacc caggaagccg cccaagagca
     1921 gatgaatctc tacctcgaag aaaacccacc gattcgcctg cgaggtgccg gcatcgtcgg
     1981 ccagagcagg accaacgccc tgatctggct gggcctgatc gtgctgatgg cagtggctta
     2041 tctactgctg gccaactgag tacccagact gggaggccgc gcaggatcgg cgttaggcct
     2101 caatcactcg gccgcagcca ccgcggtttg ccgccttaca atttgcgtgc gctgactccc
     2161 gcagcgacaa acaaagccgc cagcacagcc aacatcggca gccaaccggc gtgttcccag
     2221 atatacccgg cggcgtaacc aatcacgctg gaacccaggt aataggcgca cagatacaac
     2281 gccgaagcct gcgccttggc gccgttcgcg cgctgcccca cctggccgct ggccaccgca
     2341 tgggcggcaa agaagcccag ggtgaacaag gccaggcccg ccaccgccgc gcttaaccag
     2401 ggcgtggcac acagcgccac acccagcagc atgatggcga taccgctctg catcaccttg
     2461 cgtgcgccca agcgtggcac cagtcgcccc gcccaaccgg cgctgacgat acccagcaga
     2521 tacacggtaa acagcaggcc gatcaggctg gaagacaggt tgaacggcgc cgctgccaaa
     2581 cgaaaaccga cgtagttaaa tagtgcgaca aagccgccca tcaacagaaa tgccagcaga
     2641 aacagccggc gcaactcgcc attacccagg tgcaacgcaa agttacgcag caggccacgg
     2701 agcgacagcg actgcgctgt gaaatgccgt gatggcggca gcagccagac aaacagcccc
     2761 agcgccaaca agcccatgcc ggcaatcccg gccagggcca acggccagcc gccgagatca
     2821 ctgagcaggc cggctagcaa acgcccaagc aaaccaccta aagcggtgcc gccgatatac
     2881 agccccatcg ccgccggcag tgcctcgggg tcgaactcct cgccgacata ggccatggcc
     2941 agcgccggca atccactcag cgccaggcca agcaaggcgc gcagccagag cagatgatcc
     3001 cagctatcca ccagcgcgca ggcaatcccc agcaacgacg ccaggcccag ggccgccgcc
     3061 atcaccggct tgcgccccca actctcggcc aacgcgccgg ataccagcag gcaaacggcc
     3121 aggctcagag tggtcagcga taacgccaga ctgctgcctg ccgccgacac ggaaaaatgc
     3181 gcagccagca acggcaacag cggctgcacg caatagagca aggcaaaggt ggcaaacccg
     3241 gcgcagaaca gcaccagggt cgcacgccgg taggcggcag tgccacgcat caggtgcgca
     3301 gaggaatcga cagcaggcat cggcaggctc acaaaaaggc tctaaatcgg ccgtcctggc
     3361 agattagcac gcagccagtc ggagcggttt tgcacagcgt gcatagccct tgtgcttaat
     3421 gcgatcagta caaagcctgt ccagtgataa acgcagcgca aaaaaaaacg ggagcccaaa
     3481 cggactcccg tactcattct cagagaaatg ccggaacaag cctcacgcct gcgccagcta
     3541 caacctcacc tgcgtccccg aagaaacgca tgccgcagtg tggcaaaaaa gcctgggcaa
     3601 ctccgtttag ctttgttgcc gctttgcaaa gaaatgtaaa cgcgcgcagc ggctggccgt
     3661 gcaaagcgcc gcacggcaga ataggccgat gaatacagcc tacctcccgc cctgctgcag
     3721 ccccctgaag agtcactggc cattgccgca cagcctgccg gctctgcagt tgatcagcac
     3781 cacctttacc cccgagctgc taggtgcagc agacttcagc cgctgcgcaa tcgagccggt
     3841 gcgcggcgta gccaaacgcc aggccgaata cctggccggg cgcctgtgtg cgcgtgaggc
     3901 cttgcggcgt ttgaccggca tcgccaccac gccggcctgc ggtgaagatc gcgcgccaca
     3961 gtggccgcta ggtgtggtcg gctcaatcac ccacggcagc ggctgggccg gcgccgtggt
     4021 gggcaacagc acggattggc gcggcctagg cctggatatc gaacaactta tcccggccag
     4081 ccgtgccgag cgcctggcag cggaaatcct caccccagcc gaactgttcc gcctggaaaa
     4141 cctctccgcc gaacagcgtg cgcagcgcat cagcctgacc ttctcgctca aggaaagcct
     4201 gttcaaggcg ctctacccgc aggtattgca gcacttctac tttcaggatg ccgaactgct
     4261 cagcgtcgac gccgacaata aagtgcgtat gcgcctgctg attgacctgc atgcggactg
     4321 gcccgctggc agtgagctgg aaggccaatt cgccgagttc gacggttatc tgctcagcct
     4381 ggtcagcatc gcgcgctgat ctgtcgagcg cggcagccca aagcgctagg caaatccgct
     4441 aaactcggcc gattgccgtc tggagtgccc tatgcgcgaa gatttgaatc aaggcctgat
     4501 cgagttcctc aaggcctcgc caaccccttt tc
//
